LOCUS       DAZFJL010000001        96042 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFJL010000001 DAZFJL010000000
VERSION     DAZFJL010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 96042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.001"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            205..1776
                     /gene="garD"
                     /locus_tag="ACHJ8C_000001"
     CDS             205..1776
                     /gene="garD"
                     /locus_tag="ACHJ8C_000001"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO5549934.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPDPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(1828..2652)
                     /gene="agaR"
                     /locus_tag="ACHJ8C_000002"
     CDS             complement(1828..2652)
                     /gene="agaR"
                     /locus_tag="ACHJ8C_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO5549935.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            2901..4181
                     /gene="kbaZ"
                     /locus_tag="ACHJ8C_000003"
     CDS             2901..4181
                     /gene="kbaZ"
                     /locus_tag="ACHJ8C_000003"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO5549936.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKAAALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLIRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            4204..4677
                     /gene="agaV"
                     /locus_tag="ACHJ8C_000004"
     CDS             4204..4677
                     /gene="agaV"
                     /locus_tag="ACHJ8C_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO5549937.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDLVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            4688..5467
                     /gene="agaW"
                     /locus_tag="ACHJ8C_000005"
     CDS             4688..5467
                     /gene="agaW"
                     /locus_tag="ACHJ8C_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO5549938.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            5457..6335
                     /gene="agaE"
                     /locus_tag="ACHJ8C_000006"
     CDS             5457..6335
                     /gene="agaE"
                     /locus_tag="ACHJ8C_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO5549939.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AITVVLGIVGKFCHFL"
     gene            6353..6787
                     /gene="agaF"
                     /locus_tag="ACHJ8C_000007"
     CDS             6353..6787
                     /gene="agaF"
                     /locus_tag="ACHJ8C_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO5549940.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGSNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            6784..7917
                     /gene="nagA"
                     /locus_tag="ACHJ8C_000008"
     CDS             6784..7917
                     /gene="nagA"
                     /locus_tag="ACHJ8C_000008"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO5549941.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIALRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGHYTLCGEEVQMRGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            8267..9421
                     /gene="agaS"
                     /locus_tag="ACHJ8C_000009"
     CDS             8267..9421
                     /gene="agaS"
                     /locus_tag="ACHJ8C_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO5549942.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVKLANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESNDVITAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            9434..10294
                     /gene="kbaY"
                     /locus_tag="ACHJ8C_000010"
     CDS             9434..10294
                     /gene="kbaY"
                     /locus_tag="ACHJ8C_000010"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO5549943.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSMTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KCFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            10461..10937
                     /gene="agaB"
                     /locus_tag="ACHJ8C_000011"
     CDS             10461..10937
                     /gene="agaB"
                     /locus_tag="ACHJ8C_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO5549944.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            10976..11779
                     /gene="agaC"
                     /locus_tag="ACHJ8C_000012"
     CDS             10976..11779
                     /gene="agaC"
                     /locus_tag="ACHJ8C_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO5549945.1"
                     /translation="MHEITLLQGLSLAALVFFLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            11769..12560
                     /gene="agaD"
                     /locus_tag="ACHJ8C_000013"
     CDS             11769..12560
                     /gene="agaD"
                     /locus_tag="ACHJ8C_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO5549946.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            12561..13328
                     /gene="agaI"
                     /locus_tag="ACHJ8C_000014"
     CDS             12561..13328
                     /gene="agaI"
                     /locus_tag="ACHJ8C_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO5549947.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQIENYTALSERASEYLLAVIR
                     RKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFHSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTADRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHNHFTCVIDEMCRR"
     gene            complement(13378..14241)
                     /gene="rsmI"
                     /locus_tag="ACHJ8C_000015"
     CDS             complement(13378..14241)
                     /gene="rsmI"
                     /locus_tag="ACHJ8C_000015"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO5549948.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQENDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            14306..16342
                     /gene="lpoA"
                     /locus_tag="ACHJ8C_000016"
     CDS             14306..16342
                     /gene="lpoA"
                     /locus_tag="ACHJ8C_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO5549949.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSELTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRETT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            16300..16695
                     /gene="yraN"
                     /locus_tag="ACHJ8C_000017"
     CDS             16300..16695
                     /gene="yraN"
                     /locus_tag="ACHJ8C_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO5549950.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFIEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            16715..17305
                     /gene="diaA"
                     /locus_tag="ACHJ8C_000018"
     CDS             16715..17305
                     /gene="diaA"
                     /locus_tag="ACHJ8C_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO5549951.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            17315..17890
                     /gene="dolP"
                     /locus_tag="ACHJ8C_000019"
     CDS             17315..17890
                     /gene="dolP"
                     /locus_tag="ACHJ8C_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO5549952.1"
                     /translation="MKALSPIAVLLSALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(18004..19044)
                     /gene="yraQ"
                     /locus_tag="ACHJ8C_000020"
     CDS             complement(18004..19044)
                     /gene="yraQ"
                     /locus_tag="ACHJ8C_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO5549953.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALSFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPESQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLV"
     gene            complement(19117..19752)
                     /gene="yraR"
                     /locus_tag="ACHJ8C_000021"
     CDS             complement(19117..19752)
                     /gene="yraR"
                     /locus_tag="ACHJ8C_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO5549954.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            19880..20398
                     /gene="yhbO"
                     /locus_tag="ACHJ8C_000022"
     CDS             19880..20398
                     /gene="yhbO"
                     /locus_tag="ACHJ8C_000022"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO5549955.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGETSVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIVIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(20378..20821)
                     /gene="yhbP"
                     /locus_tag="ACHJ8C_000023"
     CDS             complement(20378..20821)
                     /gene="yhbP"
                     /locus_tag="ACHJ8C_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO5549956.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAIAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            20872..21174
                     /gene="yhbQ"
                     /locus_tag="ACHJ8C_000024"
     CDS             20872..21174
                     /gene="yhbQ"
                     /locus_tag="ACHJ8C_000024"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO5549957.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAVFAELLNSLQTPEIKSD"
     gene            complement(21161..21664)
                     /gene="yhbS"
                     /locus_tag="ACHJ8C_000025"
     CDS             complement(21161..21664)
                     /gene="yhbS"
                     /locus_tag="ACHJ8C_000025"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5549958.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(21658..22182)
                     /gene="ubiT"
                     /locus_tag="ACHJ8C_000026"
     CDS             complement(21658..22182)
                     /gene="ubiT"
                     /locus_tag="ACHJ8C_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO5549959.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKHQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            22391..23386
                     /gene="ubiU"
                     /locus_tag="ACHJ8C_000027"
     CDS             22391..23386
                     /gene="ubiU"
                     /locus_tag="ACHJ8C_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO5549960.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            23395..24273
                     /gene="ubiV"
                     /locus_tag="ACHJ8C_000028"
     CDS             23395..24273
                     /gene="ubiV"
                     /locus_tag="ACHJ8C_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO5549961.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            24354..25361
                     /gene="yhbW"
                     /locus_tag="ACHJ8C_000029"
     CDS             24354..25361
                     /gene="yhbW"
                     /locus_tag="ACHJ8C_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFO5549962.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(25479..26723)
                     /gene="mtr"
                     /locus_tag="ACHJ8C_000030"
     CDS             complement(25479..26723)
                     /gene="mtr"
                     /locus_tag="ACHJ8C_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO5549963.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIMLIL
                     VFGVGNALVHILSSFNVLPVYQ"
     gene            complement(26877..28766)
                     /gene="deaD"
                     /locus_tag="ACHJ8C_000031"
     CDS             complement(26877..28766)
                     /gene="deaD"
                     /locus_tag="ACHJ8C_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO5549964.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(28759..28839)
                     /gene="yrbN"
                     /locus_tag="ACHJ8C_000032"
     CDS             complement(28759..28839)
                     /gene="yrbN"
                     /locus_tag="ACHJ8C_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO5549965.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(28946..29830)
                     /gene="nlpI"
                     /locus_tag="ACHJ8C_000033"
     CDS             complement(28946..29830)
                     /gene="nlpI"
                     /locus_tag="ACHJ8C_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO5549966.1"
                     /translation="MKPFLRWCFIATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(29939..32074)
                     /gene="pnp"
                     /locus_tag="ACHJ8C_000034"
     CDS             complement(29939..32074)
                     /gene="pnp"
                     /locus_tag="ACHJ8C_000034"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO5549967.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(32321..32590)
                     /gene="rpsO"
                     /locus_tag="ACHJ8C_000035"
     CDS             complement(32321..32590)
                     /gene="rpsO"
                     /locus_tag="ACHJ8C_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO5549968.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(32739..33683)
                     /gene="truB"
                     /locus_tag="ACHJ8C_000036"
     CDS             complement(32739..33683)
                     /gene="truB"
                     /locus_tag="ACHJ8C_000036"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO5549969.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGRFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(33683..34084)
                     /gene="rbfA"
                     /locus_tag="ACHJ8C_000037"
     CDS             complement(33683..34084)
                     /gene="rbfA"
                     /locus_tag="ACHJ8C_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO5549970.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(34248..36920)
                     /gene="infB"
                     /locus_tag="ACHJ8C_000038"
     CDS             complement(34248..36920)
                     /gene="infB"
                     /locus_tag="ACHJ8C_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO5549971.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSVQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAGVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(36945..38432)
                     /gene="nusA"
                     /locus_tag="ACHJ8C_000039"
     CDS             complement(36945..38432)
                     /gene="nusA"
                     /locus_tag="ACHJ8C_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO5549972.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDSFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(38460..38912)
                     /gene="rimP"
                     /locus_tag="ACHJ8C_000040"
     CDS             complement(38460..38912)
                     /gene="rimP"
                     /locus_tag="ACHJ8C_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO5549973.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(39119..39195)
                     /locus_tag="ACHJ8C_000041"
     tRNA            complement(39119..39195)
                     /locus_tag="ACHJ8C_000041"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39159..39161),aa:Met,seq:cat)
     gene            39543..40886
                     /gene="argG"
                     /locus_tag="ACHJ8C_000042"
     CDS             39543..40886
                     /gene="argG"
                     /locus_tag="ACHJ8C_000042"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO5549974.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(40894..42519)
                     /gene="yhbX"
                     /locus_tag="ACHJ8C_000043"
     CDS             complement(40894..42519)
                     /gene="yhbX"
                     /locus_tag="ACHJ8C_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5549975.1"
                     /translation="MTVFNKFVKSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLIAVPFVLLIAADMSISLYSWCIFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFFAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKEAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDNQGNPTPEGQG"
     gene            complement(42979..43065)
                     /locus_tag="ACHJ8C_000044"
     tRNA            complement(42979..43065)
                     /locus_tag="ACHJ8C_000044"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43029..43031),aa:Leu,seq:gag)
     gene            complement(43080..43412)
                     /gene="secG"
                     /locus_tag="ACHJ8C_000045"
     CDS             complement(43080..43412)
                     /gene="secG"
                     /locus_tag="ACHJ8C_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO5549976.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(43640..44977)
                     /gene="glmM"
                     /locus_tag="ACHJ8C_000046"
     CDS             complement(43640..44977)
                     /gene="glmM"
                     /locus_tag="ACHJ8C_000046"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO5549977.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELTIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(44970..45818)
                     /gene="folP"
                     /locus_tag="ACHJ8C_000047"
     CDS             complement(44970..45818)
                     /gene="folP"
                     /locus_tag="ACHJ8C_000047"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO5549978.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLLDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(45908..47842)
                     /gene="ftsH"
                     /locus_tag="ACHJ8C_000048"
     CDS             complement(45908..47842)
                     /gene="ftsH"
                     /locus_tag="ACHJ8C_000048"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO5549979.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(47942..48571)
                     /gene="rlmE"
                     /locus_tag="ACHJ8C_000049"
     CDS             complement(47942..48571)
                     /gene="rlmE"
                     /locus_tag="ACHJ8C_000049"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO5549980.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            48697..48990
                     /gene="yhbY"
                     /locus_tag="ACHJ8C_000050"
     CDS             48697..48990
                     /gene="yhbY"
                     /locus_tag="ACHJ8C_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO5549981.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(49146..49622)
                     /gene="greA"
                     /locus_tag="ACHJ8C_000051"
     CDS             complement(49146..49622)
                     /gene="greA"
                     /locus_tag="ACHJ8C_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO5549982.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            49870..51303
                     /gene="dacB"
                     /locus_tag="ACHJ8C_000052"
     CDS             49870..51303
                     /gene="dacB"
                     /locus_tag="ACHJ8C_000052"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO5549983.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVTTLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(51344..52516)
                     /gene="cgtA"
                     /locus_tag="ACHJ8C_000053"
     CDS             complement(51344..52516)
                     /gene="cgtA"
                     /locus_tag="ACHJ8C_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO5549984.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKVEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(52532..53497)
                     /gene="yhbE"
                     /locus_tag="ACHJ8C_000054"
     CDS             complement(52532..53497)
                     /gene="yhbE"
                     /locus_tag="ACHJ8C_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO5549985.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLANPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(53624..53881)
                     /gene="rpmA"
                     /locus_tag="ACHJ8C_000055"
     CDS             complement(53624..53881)
                     /gene="rpmA"
                     /locus_tag="ACHJ8C_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO5549986.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(53902..54213)
                     /gene="rplU"
                     /locus_tag="ACHJ8C_000056"
     CDS             complement(53902..54213)
                     /gene="rplU"
                     /locus_tag="ACHJ8C_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO5549987.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            54472..55443
                     /gene="ispB"
                     /locus_tag="ACHJ8C_000057"
     CDS             54472..55443
                     /gene="ispB"
                     /locus_tag="ACHJ8C_000057"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO5549988.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            55678..55956
                     /gene="sfsB"
                     /locus_tag="ACHJ8C_000058"
     CDS             55678..55956
                     /gene="sfsB"
                     /locus_tag="ACHJ8C_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO5549989.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(56004..57263)
                     /gene="murA"
                     /locus_tag="ACHJ8C_000059"
     CDS             complement(56004..57263)
                     /gene="murA"
                     /locus_tag="ACHJ8C_000059"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO5549990.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(57318..57572)
                     /gene="ibaG"
                     /locus_tag="ACHJ8C_000060"
     CDS             complement(57318..57572)
                     /gene="ibaG"
                     /locus_tag="ACHJ8C_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO5549991.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(57732..58025)
                     /gene="mlaB"
                     /locus_tag="ACHJ8C_000061"
     CDS             complement(57732..58025)
                     /gene="mlaB"
                     /locus_tag="ACHJ8C_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO5549992.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEIREEAVKGITSIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(58025..58660)
                     /gene="mlaC"
                     /locus_tag="ACHJ8C_000062"
     CDS             complement(58025..58660)
                     /gene="mlaC"
                     /locus_tag="ACHJ8C_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO5549993.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(58679..59230)
                     /gene="mlaD"
                     /locus_tag="ACHJ8C_000063"
     CDS             complement(58679..59230)
                     /gene="mlaD"
                     /locus_tag="ACHJ8C_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO5549994.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(59235..60017)
                     /gene="mlaE"
                     /locus_tag="ACHJ8C_000064"
     CDS             complement(59235..60017)
                     /gene="mlaE"
                     /locus_tag="ACHJ8C_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO5549995.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRIISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(60025..60834)
                     /gene="mlaF"
                     /locus_tag="ACHJ8C_000065"
     CDS             complement(60025..60834)
                     /gene="mlaF"
                     /locus_tag="ACHJ8C_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO5549996.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            61044..62021
                     /gene="yrbG"
                     /locus_tag="ACHJ8C_000066"
     CDS             61044..62021
                     /gene="yrbG"
                     /locus_tag="ACHJ8C_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO5549997.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPREGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            62035..63021
                     /gene="kdsD"
                     /locus_tag="ACHJ8C_000067"
     CDS             62035..63021
                     /gene="kdsD"
                     /locus_tag="ACHJ8C_000067"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO5549998.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            63042..63608
                     /gene="kdsC"
                     /locus_tag="ACHJ8C_000068"
     CDS             63042..63608
                     /gene="kdsC"
                     /locus_tag="ACHJ8C_000068"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO5549999.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            63605..64180
                     /gene="lptC"
                     /locus_tag="ACHJ8C_000069"
     CDS             63605..64180
                     /gene="lptC"
                     /locus_tag="ACHJ8C_000069"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO5550000.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            64149..64706
                     /gene="lptA"
                     /locus_tag="ACHJ8C_000070"
     CDS             64149..64706
                     /gene="lptA"
                     /locus_tag="ACHJ8C_000070"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO5550001.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            64713..65438
                     /gene="lptB"
                     /locus_tag="ACHJ8C_000071"
     CDS             64713..65438
                     /gene="lptB"
                     /locus_tag="ACHJ8C_000071"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO5550002.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            65486..66919
                     /gene="rpoN"
                     /locus_tag="ACHJ8C_000072"
     CDS             65486..66919
                     /gene="rpoN"
                     /locus_tag="ACHJ8C_000072"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO5550003.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            66942..67229
                     /gene="hpf"
                     /locus_tag="ACHJ8C_000073"
     CDS             66942..67229
                     /gene="hpf"
                     /locus_tag="ACHJ8C_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO5550004.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            67347..67838
                     /gene="ptsN"
                     /locus_tag="ACHJ8C_000074"
     CDS             67347..67838
                     /gene="ptsN"
                     /locus_tag="ACHJ8C_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO5550005.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LAPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            67884..68738
                     /gene="rapZ"
                     /locus_tag="ACHJ8C_000075"
     CDS             67884..68738
                     /gene="rapZ"
                     /locus_tag="ACHJ8C_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO5550006.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            68735..69007
                     /gene="npr"
                     /locus_tag="ACHJ8C_000076"
     CDS             68735..69007
                     /gene="npr"
                     /locus_tag="ACHJ8C_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO5550007.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            69221..69853
                     /gene="yrbL"
                     /locus_tag="ACHJ8C_000077"
     CDS             69221..69853
                     /gene="yrbL"
                     /locus_tag="ACHJ8C_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO5550008.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVTPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(69850..70578)
                     /gene="mtgA"
                     /locus_tag="ACHJ8C_000078"
     CDS             complement(69850..70578)
                     /gene="mtgA"
                     /locus_tag="ACHJ8C_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO5550009.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAIFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEQA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQVWILRQMYQLGGEPFMQQHQLD"
     gene            complement(70575..71228)
                     /gene="elbB"
                     /locus_tag="ACHJ8C_000079"
     CDS             complement(70575..71228)
                     /gene="elbB"
                     /locus_tag="ACHJ8C_000079"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO5550010.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAILPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(71458..73794)
                     /gene="arcB"
                     /locus_tag="ACHJ8C_000080"
     CDS             complement(71458..73794)
                     /gene="arcB"
                     /locus_tag="ACHJ8C_000080"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO5550011.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPEDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFERMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(73890..74819)
                     /gene="yhcC"
                     /locus_tag="ACHJ8C_000081"
     CDS             complement(73890..74819)
                     /gene="yhcC"
                     /locus_tag="ACHJ8C_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562163.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO5550012.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARERGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVGLDRYLNEHGVQGSALGRPWLPPTA"
     gene            75494..79954
                     /gene="gltB"
                     /locus_tag="ACHJ8C_000082"
     CDS             75494..79954
                     /gene="gltB"
                     /locus_tag="ACHJ8C_000082"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO5550013.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEDHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            79967..81385
                     /gene="gltD"
                     /locus_tag="ACHJ8C_000083"
     CDS             79967..81385
                     /gene="gltD"
                     /locus_tag="ACHJ8C_000083"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO5550014.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAIVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEDNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(81453..81917)
                     /gene="nanQ"
                     /locus_tag="ACHJ8C_000084"
     CDS             complement(81453..81917)
                     /gene="nanQ"
                     /locus_tag="ACHJ8C_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO5550015.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKATGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(81914..82789)
                     /gene="nanK"
                     /locus_tag="ACHJ8C_000085"
     CDS             complement(81914..82789)
                     /gene="nanK"
                     /locus_tag="ACHJ8C_000085"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO5550016.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALDGDITDMVFITVSTGVGGGVVSGGKLRTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGANAKTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEIL"
     gene            complement(82786..83475)
                     /gene="nanE"
                     /locus_tag="ACHJ8C_000086"
     CDS             complement(82786..83475)
                     /gene="nanE"
                     /locus_tag="ACHJ8C_000086"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO5550017.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(83523..85013)
                     /gene="nanT"
                     /locus_tag="ACHJ8C_000087"
     CDS             complement(83523..85013)
                     /gene="nanT"
                     /locus_tag="ACHJ8C_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO5550018.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGISGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPILGALIAQRLDLGTALGSLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(85122..86015)
                     /gene="nanA"
                     /locus_tag="ACHJ8C_000088"
     CDS             complement(85122..86015)
                     /gene="nanA"
                     /locus_tag="ACHJ8C_000088"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO5550019.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(86137..86928)
                     /gene="nanR"
                     /locus_tag="ACHJ8C_000089"
     CDS             complement(86137..86928)
                     /gene="nanR"
                     /locus_tag="ACHJ8C_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO5550020.1"
                     /translation="MSPMNAFDPQAEDSTTTIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            complement(87036..87533)
                     /gene="sspB"
                     /locus_tag="ACHJ8C_000090"
     CDS             complement(87036..87533)
                     /gene="sspB"
                     /locus_tag="ACHJ8C_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO5550021.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDETHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(87539..88177)
                     /gene="sspA"
                     /locus_tag="ACHJ8C_000091"
     CDS             complement(87539..88177)
                     /gene="sspA"
                     /locus_tag="ACHJ8C_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO5550022.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(88572..88964)
                     /gene="rpsI"
                     /locus_tag="ACHJ8C_000092"
     CDS             complement(88572..88964)
                     /gene="rpsI"
                     /locus_tag="ACHJ8C_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO5550023.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(88980..89408)
                     /gene="rplM"
                     /locus_tag="ACHJ8C_000093"
     CDS             complement(88980..89408)
                     /gene="rplM"
                     /locus_tag="ACHJ8C_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO5550024.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(89628..90755)
                     /gene="zapE"
                     /locus_tag="ACHJ8C_000094"
     CDS             complement(89628..90755)
                     /gene="zapE"
                     /locus_tag="ACHJ8C_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO5550025.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTELQGQSDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            90949..91347
                     /gene="zapG"
                     /locus_tag="ACHJ8C_000095"
     CDS             90949..91347
                     /gene="zapG"
                     /locus_tag="ACHJ8C_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO5550026.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            91501..92868
                     /gene="degQ"
                     /locus_tag="ACHJ8C_000096"
     CDS             91501..92868
                     /gene="degQ"
                     /locus_tag="ACHJ8C_000096"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO5550027.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINANKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIISALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESL
                     YLLMR"
     gene            92958..94025
                     /gene="degS"
                     /locus_tag="ACHJ8C_000097"
     CDS             92958..94025
                     /gene="degS"
                     /locus_tag="ACHJ8C_000097"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO5550028.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINPRRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGM
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVNNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(94088..95026)
                     /gene="mdh"
                     /locus_tag="ACHJ8C_000098"
     CDS             complement(94088..95026)
                     /gene="mdh"
                     /locus_tag="ACHJ8C_000098"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO5550029.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            95461..95931
                     /gene="argR"
                     /locus_tag="ACHJ8C_000099"
     CDS             95461..95931
                     /gene="argR"
                     /locus_tag="ACHJ8C_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO5550030.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKELYEAILELFDQEL"
BASE COUNT        23415 a        25859 c        24107 g        22661 t
ORIGIN      
        1 gagcatatgc acataaggtt gcgcgctaaa gcacagattt gcgctttacc ttgccgggag
       61 gtactgcaat ccctgaaatg attgacattg atcacatttc tgcatttaaa ctcctgacat
      121 tcttatttca cccaatgaag ttatttattt ttaaatgaga ccaggtcctc attttaatag
      181 tccctggctg gagaatattg cacaatggcc aacatcgaaa tcagacaaga aacgccaact
      241 gcgttttata taaaagttca cgacacagat aatgtggcaa ttattgttaa tgataatggc
      301 ctgaaagcag gaacgcgttt tccggatggg ctggaattaa ttgaacatat tccccagggg
      361 cataaagtcg cattgctgga cattccggct aatggtgaaa ttattcgtta tggcgaggtg
      421 attggttacg ccgtgcgcgc aatcccacgc ggaagctgga tcgacgaatc aatggttgta
      481 ctaccggaag cgccgccgtt acatacgctg ccactggcaa ccaaagtccc ggacccctta
      541 ccgccgctgg aaggatacac ctttgagggc tatcgcaatg ccgatggcag cgtgggcacc
      601 aaaaacctgc tcggcatcac caccagcgtc cactgtgtgg caggcgtggt ggactacgta
      661 gtaaaaatca ttgaacgcga tctgctaccg aaatacccga acgtcgatgg cgtggtgggg
      721 ctgaatcatt tgtacggttg tggcgtggcg attaacgcac ccgcggcagt tgtgcctatt
      781 cgtaccattc acaatatttc gctgaaccct aactttggcg gcgaagtaat ggtgattggt
      841 ctgggttgtg aaaagttgca gcctgaacgc ctactgactg gaacggatga tgtgcaagct
      901 attccagtag aaagcgccag cattgtcagt ttgcaggatg aaaagcatgt cggttttcag
      961 tccatggtcg aggatatttt gcaggtcgcc gaacgccatc tacaaaaact gaatcaacgt
     1021 cagcgagaaa cctgtccggc ttcagaactg gttgtcggca tgcagtgcgg tggcagcgat
     1081 gcgttttctg gcgtaacggc aaacccggcg gttgggtatg cgtctgatct attggtgcgc
     1141 tgcggcgcaa cggtgatgtt ttcagaagtc acagaagtgc gtgacgcgat acatctgctg
     1201 acaccacgcg cagtgaacga agaggtcggc aaacggctgc tggaggagat ggagtggtac
     1261 gataactatc tcaatatggg aaaaaccgat cgcagcgcca acccttcgcc ggggaacaag
     1321 aaaggcggtc tggcaaatgt ggtggagaag gcactcggct ccattgctaa atcgggtaaa
     1381 agtgcaattg ttgaagtgct gtcgcccggc caacgcccga ctaaacgcgg attaatttac
     1441 gccgcgacgc cagccagcga ttttgtctgt ggcacgcaac aggtggcttc gggtatcacc
     1501 gtgcaagtgt ttacgaccgg tcgtggtacg ccgtacggcc tgatggcggt acccgtcatt
     1561 aaaatggcga cccgcaccga gctggcgaac cgctggtttg atttaatgga tattaacgcg
     1621 ggcactatcg ctaccggcga agaaaccatt gaagaagtgg gctggaagtt gttccacttt
     1681 attctcgacg tcgccagcgg gaagaagaaa actttctcgg atcaatgggg actgcataac
     1741 cagctggcgg tgtttaaccc ggcaccggtg acctgataat tcaattcgta ttatgcccga
     1801 tagcacacgt ttatcgggca tattgagtca cagcgaagac gcgtgatccc cgaccagaat
     1861 cacttcaacc ccagcctttc tcagtccttc cagactatcc gcaggaatgc cttcatcaac
     1921 aatgatcatg tcgatacgtt gagtatcaat gatcttatgt aaactagaac gattgaactt
     1981 actggaatcg gtgaccacga tgatccgttc cgcaacttcg cacatccgac ggtttaaacg
     2041 ggcttcatct tcattatgcg tgctgacgcc gcgctccaga tcgatcgcat ctacaccaag
     2101 aaacagcata tcgaagtggt aattttgcag cgattgctca gcctgatcac cgtaaaaaga
     2161 ttgcgactga cggcgcaaat gccccccggt catcagcagc tcaacgcctt ccgcttccag
     2221 caacgcatta gccacgttca taccgttggt catcgcaatt acgtcggtgt gcttgcgcat
     2281 cagacgagca atctcaaaag tggtggtccc ggagtcgagg atcacccgat gaccaggctg
     2341 aatcaactca acggcagctt tcgcaacgct gcgtttcatc gcggtgttca gcgcgctttt
     2401 atcttccact gatggctcga ctgacggcgt tgtgctatcg cagatcaacg cgccaccata
     2461 ggcacgcaca gcgatcccct gcttttccag aaacgccaga tcgttgcgga tcgtcacagt
     2521 agatacacca tacaacgccg atagatcatt aacttgcaca cttccttgct ggcgcaggcg
     2581 ttgaatgatc tgctctcgtc gctcactggt gcctgtcact cgcttctcac ctgaagcgtc
     2641 ggtattactc atagtaagtc ctttcgtaaa actttcgttt catttcgttt tgcctattaa
     2701 cgcctttcta ttaagcaaat gcaagcccac cttacccatt ggcgcaagct actctcgttt
     2761 cagtggcttt cattatgttt cttttgtgaa tcagatcaga aaaccattat ctttcgtttt
     2821 atttttatct caccatgacg cagtatcaac tgaaacaaaa cgaaagatta atgtcgcagt
     2881 aatctgaact ggagaggaaa gtgaaacatc tgacagaaat ggtgagacag cacaaagcgg
     2941 gcaaaacaaa tgggatttac gccgtttgtt ccgcgcatcc gctggtgctg gaagctgcaa
     3001 tccgctacgc cagtgcaaac caaacgccgt tactgattga agcaacctcc aatcaggtgg
     3061 accagttcgg cggttatacc ggaatgacgc ccgccgattt tcgcggcttt gtttgtcagc
     3121 tcgccgactc gttgaatttc ccgcaggatg cgctgattct gggtggtgac catctggggc
     3181 caaaccgctg gcaaaacctg cctgccgctc aggcaatggc caatgccgat gatttgatta
     3241 aaagctacgt ggcggcagga ttcaaaaaaa ttcaccttga ttgcagcatg tcctgtcagg
     3301 acgatccgat tcccttaact gatgacatcg tggctgaacg cgccgcccgt ctggcgaaag
     3361 tggcggaaga aacctgtcgt gaacactttg gcgaagccga tctggagtat gtcattggta
     3421 ccgaagtgcc ggtacctggc ggcgcgcatg aaaccttaag cgagctggcg gtcaccacgc
     3481 cggatgccgc ccgcgccacg ctggaagccc atcgtcacgc ctttgaaaag caaggtctga
     3541 atgccatctg gccacgcatc attgccctgg tggttcaacc cggcgtcgaa ttcgatcaca
     3601 ccaacgttat tgattatcag ccagccaaag ccgctgcctt aagccagatg gtcgaaaact
     3661 acgaaacgct gattttcgaa gcgcactcta ccgattatca aacgccgcaa tcgctgcgcc
     3721 agctggtgat tgaccacttt gccattctga aagttggccc ggcgctgacc ttcgccctgc
     3781 gtgaagctct gttctctctg gcggcgattg aagaagaact ggtgccagcg aaagcctgtt
     3841 ccggtctgcg tcaggtactg gaaaacgtga tgctcgaccg cccggagtac tggcaaagcc
     3901 actaccacgg tgacggcaac gcgcgtcgtc tggcgcgtgg ttatagctac tcggatcgcg
     3961 tgcgctatta ctggccggac agccagattg atgacgcttt cgctcatctg attcgtaatc
     4021 tggcggattc accaattccg ctgccgctga tcagtcagta tctgccgctg cagtacgtga
     4081 aagttcgctc cggcgagctg cagccaacgc cacgggaact cattatcaac catattcagg
     4141 acatcctggc gcagtaccac acagcctgtg aaggccaata agcaaaacaa agaggaacac
     4201 gctatgccaa atattgtttt aagtcggatt gatgaacgct tgattcacgg tcaggtcggc
     4261 gttcagtggg tcggatttgc gggggcaaat ctggtgctgg tagccaacga cgaggttgcc
     4321 gaagatctgg tacaacaaaa cctgatggaa atggtactgg cagaagggat cgccgtacgt
     4381 ttctggacgc tgcaaaaagt catcgacaac attcatcgcg ccgccgatcg acagaaaatc
     4441 ctgctggttt gtaaaacacc ggccgatttc ctgacgctgg tgaaaggtgg cgttccggtg
     4501 aatcgcatta acgttggcaa tatgcactac gccaatggca agcaacaaat cgccaaaacg
     4561 gtttctgtgg atgcgggtga tatcgcagca tttaacgacc tgaaagccgc tggggtggaa
     4621 tgcttcgttc agggcgtccc aacagagcct gctgtggacc tctttaaatt actttgaggg
     4681 attcatcatg gaaatcagtc tgttgcaggc atttgcgttg ggcattatcg cctttatcgc
     4741 tggcctggat atgtttaacg gcttaaccca tatgcaccgc ccggtggttc tcggcccgtt
     4801 ggtcgggctg gtacttggcg atctgcatac cggtatttta accggcggta cgctggaact
     4861 ggtgtggatg gggctggccc ctctggcggg cgcacagccg cctaacgtga ttatcggtac
     4921 tatcgtcggc acggcgtttg ccattactac tggcgtgaaa cccgatgtcg cggtcggtgt
     4981 cgccgtacct ttcgctgtcg cagtacagat ggggattacc ttcctgttct cggtgatgtc
     5041 cggcgtgatg tcccgatgcg accggatggc ggagaacgcc gatacacgcg gcattgaacg
     5101 cgtgaactat ctggcattgc tggcactcgg cacgttctat tttctctgcg ctttcctgcc
     5161 tatctacttc ggcgcggaac atgcaaaaac catcattgat gtcctgccgc aacgattaat
     5221 tgatggcctc ggcgtcgcgg gcggcatcat gccagcaatc ggttttgccg tgctgctgaa
     5281 aatcatgatg aaaaacgtct acatccccta cttcatcctg ggctttgttg ccgcggcctg
     5341 gctcaagtta ccggtgctgg ctattgctgc cgctgcgctg gcgatggcgc tgatcgacct
     5401 gctgcgtaaa tctcctgaac cgactcaacc tgcggcacag aaagaggaat ttgaagatgg
     5461 catctaatca aaccaccctg ccgaacgtca gcgaaaatga agaaacactg ctgactggcg
     5521 tcaatgaaaa cgtgtatgaa gatcagagca ttggcgcgga gctgacgaaa aaagatatca
     5581 accgcgtcgc ctggcgttcc atgctgttac aggcttcttt caactacgaa cgtatgcagg
     5641 cttccggctg gctgtacggc ctgctgccag cactgaaaaa gatccacact aataaacgcg
     5701 acctggcgcg cgccatgaag gggcatatgg gtttcttcaa tacccatccg tttctggtga
     5761 cgtttgttat cggcattatc cttgcgatgg agcgttctaa gcaggacgtt aacagtattc
     5821 agagcaccaa aattgccgtc ggtgcgccgc tcggcgggat tggcgatgcg atgttctggc
     5881 taacgctact gccgatttgt ggcgggatag gtgcaagcct cgcgctacaa ggctctattc
     5941 ttggcgctgt cgtctttatt gtgctgttca acgtggtgca cctggggctg cgttttggtc
     6001 tggcgcatta tgcttaccgc atgggcgtgg cggcgattcc actcattaaa gcaaatacca
     6061 aaaaagtcgg ccatgctgca tctatcgttg ggatgacggt aatcggcgcg ctggtggcaa
     6121 cctatgttcg tttaagcacc acgctggaaa tcaccgcggg cgacgcagtg gttaagttac
     6181 aggctgatgt tatcgacaaa ctgatgccag ccttcttacc gctggtctac accctgacca
     6241 tgttctggct ggtacgccgc ggctggagtc cgctgcgcct gattgcgatt accgtggttc
     6301 tcggcatcgt cggtaaattc tgccatttcc tttaaataca atgaggtatt agatgttaag
     6361 tattattttg acagggcatg gcggatttgc cagcggcatg gaaaaagcaa tgaagcaaat
     6421 tctcggtgag caatcgcagt ttatcgccat cgattttccg gaaacctcaa gcaccgcgct
     6481 gcttacatcg cagcttgaag aggccatcgc ccaactggat tgtgaagacg gcattgtttt
     6541 tctgacggat ttgctgggcg gcacgccgtt tcgcgtagct tcaacgctgg cgatgcaaaa
     6601 accgggctgt gaagtgatca ccggctccaa tttgcaactg ctactggaaa tggtgctgga
     6661 gcgcgaaggg ttaagcggtg aagagttccg tgtgcaggcg ctggagtgtg gacatcgtgg
     6721 gctgaccagt ctggtggacg agttaggccg ctgccatgaa gaatgtccgg tcgaggaagg
     6781 aatatgacac acgttctgcg cgccagaagg ctgctgactg aagagggatg gctcgatgac
     6841 catcagttgc gtattgctga cggtgtaatc gcagcaatcg aaccgattcc agtgggcgtg
     6901 actgaacgcg atgcggaact gctctgtccc gcttacatcg acacccatgt acacggtggt
     6961 gcgggcgttg atgtaatgga tgacgcgccg gatgtgctcg acaagctggc aatgcacaaa
     7021 gcacgcgaag gtgtcggcag ttggttaccg accaccgtaa ccgcgccgct taataccatt
     7081 catgcggcgc tgaaacgtat tgctctacgc tgccagcgtg gcggacctgg tgcgcaagtg
     7141 ctggggagtt atctcgaagg accgtacttc acgccgcaga ataaaggcgc gcatccgccg
     7201 gagctgtttc gcgagcttga aattgccgag ctggatcaat tgattgccgt ttctcagcat
     7261 accttgcgcg tggtagcgct ggcaccagaa aaagaagggg cattgcaggc catccgccat
     7321 cttaaacagc aaaacgtacg ggtgatgctg gggcatagcg cggcgacctg gcaacaaacc
     7381 cgtgccgcgt ttgatgctgg tgccgacggc ctggtgcatt gctataacgg gatgacaggt
     7441 ttacatcacc gcgaaccggg aatggttggc gcgggattaa cagacaagcg cgcctggctg
     7501 gaactgatag ccgatggcca tcatgtacat ccggcggcaa tgtcgctctg ttgttgctgt
     7561 gcgaaagaga ggatcgtact gatcaccgac gcgatgcagg cagccgggat gccggatggt
     7621 cactatacgt tatgtggtga agaagtgcag atgcgcggtg gcgttgtccg taccgcgtcc
     7681 ggtgggctgg cgggcagtac gctgtctgtc gatgcggcag tgcgcaacat ggtcgagttg
     7741 acgggcgtaa cgcctgcgga agccatccat atggcgtcgc tgcatccggc gcgaatgctg
     7801 ggtgttgatg gtgttctggg atcgctaaaa ccgggcaaac gcgccagcgt cgttgcgctg
     7861 gatagcgggc tgcatgtgca acaaatctgg attcagggtc aattagcttc gttttgatgg
     7921 tttgctcctt tattaggcct tcacttcccc cgtaaggcct gtctttttct ttcgttttga
     7981 tctgtgcagc ggtgtcggat gcgacgctaa tgcgtcttat ccgacctacg gttggtgacc
     8041 gtaaggccgg ataaagtatt tacgccgcat ccagcaatcc cttttacttc ccttgccttt
     8101 tctttcaacg atcacagatt tcgttttatt tcctttttct tcattgaact ttcagtttct
     8161 tttctataaa ttttaaccaa cgaaagaaat caccaagtga aatgaaacga aaggcaagcg
     8221 aaagcgacaa cgccgacgtc aagttcatca gactaaggat tgagttatgc cagaaaatta
     8281 cacccctgct gctgccgcaa ccggtacatg gactgaagaa gagatccgcc atcagcctcg
     8341 cgcatggatc cgttcactca ccaacatcga cgcgctacgt tccgcgctca ataacttcct
     8401 tgaaccgtta ctgcgcaaag agaatctgcg gatcatcctg accggagccg gaacctcggc
     8461 atttatcggt gacatcatcg cgccgtggct cgccagccat accggtaaaa acttcagcgc
     8521 cgtaccgacc accgatctgg tcactaatcc gatggactac ctgaatccag ctcatccgct
     8581 gctgttgatc tccttcggtc gatccggcaa cagcccggaa agcgtcgccg ccgtgaaact
     8641 ggcaaatcaa tttgtaccag aatgctatca cctgccgatc acctgcaacg aagcgggcgc
     8701 tctttaccaa aacgcgatca acagcgacaa cgcgtttgcc ctgctgatgc ccgcagaaac
     8761 gcacgatcgc ggcttcgcga tgaccagcag cattaccacc atgatggcca gctgcctcgc
     8821 ggttttcgca cctgagacga tcaacagcca aaccttccgc gatgtggcgg atcgttgcca
     8881 ggcgatcctg acctcactgg gcgatttcag cgaaggtgtg tttggttacg caccgtggaa
     8941 acggatcgtt tatctcggca gcggtggctt acagggcgca gcacgcgagt cggcgctgaa
     9001 agtgctggaa ctgacagcgg gtaaactggc ggccttctat gattccccga ccggattccg
     9061 tcatggcccg aaatcgctgg tcgataacga aacgctggtg gtggtgtttg tctccagcca
     9121 cccttacacc cgtcagtatg atcttgatct gctggcagaa ctccgccgcg ataaccaggc
     9181 aatgcgcgta atcgccatcg ccgcggaaag caacgacgtt attaccgccg gtccacatat
     9241 catcctgccg ccgtcccgtc actttatcga cgttgagcag gcattttgct tcctgatgta
     9301 cgcccagaca tttgcactga tgcagtcgct gcacatgggt aatacgccgg ataccccatc
     9361 agccagtggc accgttaacc gcgtggtgca aggcgtaatc attcatccgt ggcaggcata
     9421 agaggatcgc attatgagca ttatctccac caaatatctg ttacaggacg cccaggccaa
     9481 tggctacgcg gtgcctgcct ttaacattca taacgccgag acgatccagg cgatcctcga
     9541 agtgtgcagt gaaatgcgat cgccggtgat cctcgccggg acgccgggga cctttaagca
     9601 tatcgcgctg gaagagatct acgccctgtg tagcgcctat tccatgacct ataacatgcc
     9661 gctggcactg catcttgatc accacgaatc gctggatgat attcgccgta aagtccacgc
     9721 aggcgtgcgc agcgcgatga tcgacggcag tcatttcccg tttgccgaga acgtgaagct
     9781 ggtgaaatcg gttgtcgact tctgccactc ccaggattgc agcgtggaag cagaactggg
     9841 ccgcctcggc ggtgttgaag atgacatgag cgttgacgcc gaaagtgcat tcctgaccga
     9901 tccacaagaa gctaaatgct ttgtcgaact gactggcgtc gacagcctgg cggtagcgat
     9961 tggtacggcg cacggcttat acagcaaaac gccgaagatt gatttccagc ggctggcgga
    10021 aattcgtgaa gtggtcgatg ttcctctggt gctgcatggt gccagcgatg tcccggatga
    10081 atttgtccgt cgcactattg aacttggcgt aacaaaagtg aacgttgcca cagaattaaa
    10141 aatagccttc gctggcgcgg ttaaagcctg gtttgcggaa aatccgcagg gtaatgatcc
    10201 tcgttattat atgcgcgtcg gaatggatgc gatgaaagaa gttgtcagaa ataaaattaa
    10261 tgtctgtggt tcagcgaatc gaatttcagc ataatcatta gttttttatg atttatccca
    10321 atgtacttcc cgattaatat cggggagtgc cttaatggta aaggagataa ctaaacctta
    10381 ataaatacat cagtacaata tcgcaacaat aatatattta aaaagattat attattcaac
    10441 tttatggtga ggattacaca atgagcagtc caaatattct cttaacccgt attgataacc
    10501 gtctggttca cggtcaggtt ggcgtgacct ggacatccac catcggtgcg aatctgttag
    10561 tagtcgtgga tgatgttgtc gctaacgatg atattcaaca gaaattaatg ggtattaccg
    10621 cagaaaccta cggctttggc attcgtttct ttactatcga aaaaaccatt aacgtcatcg
    10681 gcaaagctgc accacatcag aagatcttcc tgatttgccg tacgccacaa acggtacgta
    10741 aattggtaga aggtggtatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg
    10801 aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc acggatttac
    10861 gttttattaa acaacgtggc gtaaatgttt tcattcagga cgtccctggc gatcaaaaag
    10921 aacaaatccc tgactaaatc taaaatagcc ttaatattgg tttgaggtaa taaaaatgca
    10981 tgaaataacc ctactccagg gattatccct ggcggcgtta gttttctttc tggggattga
    11041 tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc ttacaggtgc
    11101 tattctcggt gatattcaga ctggcttaat taccggtggc ctgacagagt tggctttcgc
    11161 cggattaacc cctgcaggtg gtgttcagcc gcccaaccct attatggcgg gtctgatgac
    11221 cactgtcatt gcatggtcta cgggcgttga tgccaaaacg gcaattggtc ttggcctgcc
    11281 gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat
    11341 gaccaaagcc gataaatgcg caaaagaggc ggatacggca gcgttttccc gacttaactg
    11401 gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct
    11461 ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggctga cccacggctt
    11521 tgaagtggct ggcggtattc tgcctgctgt tggttttggc ttgctgctgc gcgtgatgtt
    11581 caaagcacaa tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccaggt
    11641 cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa
    11701 tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga
    11761 ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt
    11821 cactgctaca agcaagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga
    11881 tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcctaagc gcggcgatga
    11941 aagataacct cgaatttatt aatacccacc ctaatctggt cggattcctg atggggttat
    12001 taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcgc
    12061 tgtttggccc aatcgccggg attggcgatg cgattttctg gtttaccttg ttgccaatta
    12121 tggcggggat ttgctcatcg tttgccagtc aggggaacct gttgggaccg attttgtttt
    12181 tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag
    12241 tcggcgtgaa ggctatcgat aaagtgcgag agaactcgca gatgattgcc cgttcggcaa
    12301 ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg
    12361 tgacgtcgtt tgccatcgac agtacccaca gcgtcgcact gcaacaggat ttcttcgata
    12421 aagtcttccc gaatatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg
    12481 tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacgtt tgttctttct attgtttgtt
    12541 ctgcattcgg cattttgtaa atggaacgag gcaccgcgtc tggcggtgcc tctttactta
    12601 aggaatttca tcctgtgcaa acccttcaac aaattgaaaa ctatacggcg ttaagtgaac
    12661 gtgccagcga atatttattg gccgtgatcc gtcgcaaacc ggatgccgtg atttgcctgg
    12721 cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc
    12781 aggttgatgt cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa
    12841 cgatgcctgg tacctgcgaa actttccttc aacagcatat cgtgcagccg ctggggctac
    12901 gtgaagacca gctcatcagc tttcactccg aagagataaa tgagacagag tgcgaacggg
    12961 taacgaacct gattgcgcgc aaaggcggtc tggacttatg cgttctcgga ttggggaaaa
    13021 acggtcatct tgggctgaac gaaccgggag aaagcctgca accggcctgc catatcagtc
    13081 aacttgatgc cagaacacag caacatgaga tgttaaaaac cgcggaccgt cccgtgactc
    13141 gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaagttttg ttactggtga
    13201 ctggcgaagg aaaacaggat gcgacagaac gttttctcac ggctaaagtc tccaccgcta
    13261 tcccggcttc atttttatgg ctgcataacc atttcacatg cgtgattgat gaaatgtgtc
    13321 gtcgttaact cgccaggtaa aggcgttgta gcgctattcc tgctgcgacg cctgtaatca
    13381 ctctccctgc tgctccagcg catacttata cagcgcattt ttcttcacgc cgtgaatttc
    13441 tgcggccagc gccgccgctt ttttcagcgg cagttctgcc tgtagcagcg ccagcgtgcg
    13501 cagggcatca gcaggcaagt cattttcctg cgctttatgg ccttcgacaa tcagcaccat
    13561 ttcgcctttg cgacggtttt catcttcttt tacccacgcc agcagctcgc caacgggcgc
    13621 gccgtgaatg gtttcccagg ttttggttag ctcacgcgcc agaaccacgt aacgggattc
    13681 gcctaatacc gcaacaatat cttccaggct atctaacaga cggtgggtag attcataaaa
    13741 aatcagcgtg cgcggctccg cttcaatagc ttttagcgca tcacggcggc cttttgattt
    13801 ggcaggtaaa aagccttcat aacaaaaacg atcggatggt aaacccgcgg cgcttaacgc
    13861 agtgatagca gcacacggcc ctggtaacgg caccacgcga atccccgctt cacgacaggt
    13921 acgcaccaga tggtagccag gatcgttaat tagcggcgtt ccggcatcgg aaaccagcgc
    13981 aatattttgc ccctcttgca gcttcgccag cagcgtttcg gctttttgtt gttcgttatg
    14041 gtcgtgcagc gcaaacaacc gggcattaat cccaaaatgt tgcagcaata aaccggtgtg
    14101 acgcgtatcc tcggcggcaa tcagatcaac ggcctgtaat acctctaatg cacgctgggt
    14161 gatatccgcc agattgccga ttggcgtcgg tacaatgtaa agctgcccct gagaattatc
    14221 cgccgattgg tgttgtttca ttgtgtcgtc cgtattgccg atttaatatt gagcattgcg
    14281 taaaaaaaat atcactggat acattatggt accctcaaca ttttctcgtt tgaaagccgc
    14341 gcgttgtctg cctgttgttt tggcagccct gattttcgcc ggttgtggca cccatactcc
    14401 cgatcagtcc actgcttata tgcagggcac ggcgcaggct gattctgcct tttacctgca
    14461 acagatgcag caaagttctg atgataccag gatcaactgg caattactcg ccattcgtgc
    14521 actggtgaaa gaaggtaaaa ccggacaggc ggtggagttg tttaaccaac taccgcaaga
    14581 actgaacgat tctcagcgtc gcgagaaaac actgctggcg gcagagatta aactggcgca
    14641 gaaagatttt gctggcgcgc aaaacttgct ggcgaaaatc acacctgccg atttagaaca
    14701 aaaccagcaa gcccgttact ggcaggcaaa aatcgatgcc agccaggggc gtccttccat
    14761 tgatttactg cgcgcgttaa ttgctcagga accgctgctc ggtgcgaaag aaaagcagca
    14821 gaatattgac gccacatggc aggcgctctc ctccatgact caggagcagg cgaatacgct
    14881 ggtgatcaac gccgacgaaa atattctgca aggctggctg gatttgcagc gcgtctggtt
    14941 tgataaccgt aacgatcctg acatgatgaa agctgggatc gccgactggc agaaacgtta
    15001 tccgaacaac ccgggcgcga aaatgctgcc aacgcagttg gttaacgtaa aagcgtttaa
    15061 accagcatcg accaacaaaa tcgccctgct gttgccgctg aatggccagg cagcggtatt
    15121 tggtcgcact attcagcaag gctttgaagc ggcgaaaaat atcggcactc agccagtggc
    15181 ggcccaggta gcagccgcac ctgccgcaga cgtagcagaa caacctcagc cgcaaaccgt
    15241 ggatggcgtt gccagcccgg cacaagcttc ggttagcgag ctgaccggtg aacagcctgc
    15301 ggcccagccg gtacctgtaa gcgccccggc gacaagcacc gcagcggtaa gcgcacccgc
    15361 aaatccatcc gcagagctga aaatctacga tacctcatca caaccactta gccagatctt
    15421 aagccaggtt cagcaggatg gtgcgagtat tgtggtcggt ccgttgctga aaaataacgt
    15481 tgaagagttg ctgaagagca acactccgct gaacgtgctg gcactgaacc agccggagaa
    15541 catcgaaaac cgcgtcaata tttgttactt cgcgctttca ccggaagacg aagcgcgcga
    15601 tgcagcgcgt catattcgtg accagggtaa acaagcgccg ctggtgctga tcccacgcag
    15661 ttcattgggc gatcgcgtag ccaatgcgtt tgcgcaagag tggcagaaac tgggcggcgg
    15721 caccgttctg caacaaaaat ttggttccac cagcgagtta cgcgcgggtg ttaacggcgg
    15781 ttcgggtatc gctttaacgg gtagcccgat tactcccaga gaaacaaccg actccggcat
    15841 gacgaccaac aatccaacgc tgcaaaccac accaaccgat gatcagttca ccaataatgg
    15901 cggtcgtgtc gatgcggtgt acattgtggc aacgccgggt gaaatcgctt ttatcaaacc
    15961 aatgatcgcc atgcgtaacg gtagccagag cggtgcaacg ctgtacgcca gctcccgcag
    16021 tgcgcaaggg accgctggcc cggatttccg cctggagatg gaaggcttgc agtacagcga
    16081 aatcccgatg ctggcaggcg gtaatctgcc gttaatgcag caggcgctca gcgcggtgaa
    16141 caacgattat tcgctggctc gcatgtatgc gatgggcgtc gatgcctggt cgttggcaaa
    16201 tcatttctca caaatgcgcc aggttcaggg ttttgaaatc aacggtaata ccggaagcct
    16261 gacggcaaac ccggattgcg tgattaacag gaagttatca tggctacagt accaacaagg
    16321 tcaggtagtc cccgccagtt aaccaccaaa cagaccggcg atgcgtggga agcacaagcg
    16381 cgtcgctggc tggaaggcaa aggactacgg tttatcgccg ctaacgtgaa cgagcgtggc
    16441 ggcgagatcg atctgataat gcgtgaaggc cggaccaccg tttttatcga ggtgcgttac
    16501 cgccgctctg cgctttatgg cggcgcggca gccagtgtga cccgcagcaa acaacacaaa
    16561 ttattacaga ctgcccgctt gtggctcgcg cgtcataatg ggagttttga tactgtggat
    16621 tgccggttcg atgtggtagc cttcaccggg aatgaggttg agtggattaa ggatgccttt
    16681 aatgaccact cataattaag gtttaaggat tagcgtgcaa gaaagaatta aagcttgctt
    16741 cactgaaagc attcaaactc aaattgcggc ggcagaggcg cttccggatg ccatctcccg
    16801 tgcagccatg acgctggttc agtctctgct caatggcaac aaaatcctct gttgtggtaa
    16861 tggaacttcc gctgccaatg cacagcattt tgctgccagc atgatcaacc gtttcgaaac
    16921 ggagcggccc agcttacctg ccattgcact aaatactgat aatgttgtct taacggcaat
    16981 tgccaacgat cgcttacatg atgaagtgta tgcaaaacag gtgcgggcgc tgggtcatgc
    17041 gggagatgta ttgttagcca tttccacccg tggcaacagc cgcgatattg taaaagcggt
    17101 agaagctgcc gttacgcgtg atatgaccat tgtggcatta accggctatg acggcggtga
    17161 acttgcaggt ttgttagggc cacaggatgt ggagatccgc attccttcgc atcgtagtgc
    17221 tcgcattcag gagatgcata tgctgacggt aaattgcctg tgcgatctga tcgataacac
    17281 gcttttccct caccaggatg tttaaggaga atacatgaag gcattatcgc caatcgcagt
    17341 ccttctttcc gcgctgctgt tgcaaggttg tgttgccgct gccgtagtgg gtaccgctgc
    17401 tgtgggtacc aaagccgcaa cggacccacg cagtgtcggc acccaggtgg acgatggtac
    17461 cctggaagtg cgcgtaaaca gcgcattgtc gaaagacgaa cagattaaga aagaaacccg
    17521 tattaatgtc acggcgtatc agggcaaagt gctgttggtt gggcagtcac caaacgctga
    17581 actttcggct cgcgctaaac aaattgcgat gggcgtagac ggtgccaacg aagtgtataa
    17641 cgagattcgt cagggccagc cgattggtct gggcgaagcg tctaacgata cgtggatcac
    17701 caccaaagtg cgttcgcagc tgttaaccag cgacctggtg aaatcgtcca acgtgaaagt
    17761 gaccaccgaa aacggtgaag tgttcctgat ggggctggtg actgaacgtg aagcgaaagc
    17821 agcggcagat attgccagcc gggttagcgg cgtgaagcgt gtaaccacag cgtttacgtt
    17881 tattaaatag cagccccttg taatgcctga tgcgacgctt gccgcgtctt atcaggccta
    17941 caacgatgca aaccgtaggt cggataaggc gtttacgccg catccgacat gttttccctc
    18001 aaatcaaacc aacagcgcca gcccgccgac aatcacacca gacactgcca ccatcgcccc
    18061 cgtcagccat aaggctttcg ccgggaacgc tttgcgcagc ataatcagcg acggcaaact
    18121 caccgccggg agcgtcatca acaatgccag cgccggagca gttcccatac ctgccagcat
    18181 catcgtttgt acaatcggaa tttctgctgc cgtgggaatg acaaacaagc atcctgccac
    18241 cgccatcgcc accacccaca tcaggctgtt atcgacagca ccatcggcat gggggaataa
    18301 ccagacgcga gcggcaccca acaccagtac tgcaaggatg taaaccggga tcgtactcca
    18361 gaaaagcgtc catagcgccc tgccccagcg gctaaaaaat cctccctgtg attccggtat
    18421 gtcaatttcg accggtgcct gcgtttgcgg tgtttcacgc acccattttt gcaccagcgt
    18481 cgcaatcagc aacaccatca ccaggcccgc caccagacga atcgccgcaa aaccccagcc
    18541 gaggacgaac cccataaaca ccagcgtagc cgggtttaac aacggattcc ccatccagaa
    18601 tgacagcgca ccgcccatcg acacctgttg gcgacgcatt cccgccgcga ccggagccgc
    18661 acagcaggta cacatcatgc ccggcagcga aaacagcgtt cccagcaacg tgccgcgaaa
    18721 gcgcgattgc ccaagcgtac gcaacaacca gtcacgcggg atcagcacct gaatcaacga
    18781 accgaggatc acccccagca ccgccgcttt ccataccgcg agaaaataga tcatcgcgta
    18841 atccaacgcc gcctgccacg gattagcatc cgcctgcgca aggatagatt taccgatact
    18901 gtgggtttcg gcagcagtaa acgctttgcc gtagtaaggt tgccatttca cataccagag
    18961 accggcaatg acaacgagaa agaaaagcgc gggtttccac cactgaatgg gcgttgccgc
    19021 ctgagatgaa gactgaccag tcatagcatt ccccaggaat aagttatgat aattgagcgc
    19081 gtgaatatta cgctcactat caattcttgg gaataattat tcagctcttt tgcgtaattc
    19141 tgaagagctt aaaatcgtca caccttcatg ctcaggccgc atcgcctctg ccagcatgac
    19201 ccgcgccacg tctctcgcat caatggattt ccagttacca ggtaacaaac ggaacagcgg
    19261 cgcaaaaagc gtttcgttca tccgctgttt gctacgatcg cccagtaaca tcgacgggcg
    19321 agcaatggtc aatttcggcc agttctgggc gattaatgct tcttccatct cccctttgac
    19381 gcggttatag aaaaacggcg agtgggcatt ggcacccata gcactaacca ccagcatatg
    19441 ctgagcgccc agtcgccgcc cggttaatgc ggtgtccaca accaacgtgt aatcggcatg
    19501 aataaacgcc tctttgctgc ccgcttcccg ccgcgtggtg ccgagacaac aaaacacaat
    19561 gtcgatggga tcggtgacct gcgccagcgc gtcggtcagt tgcgggtcat ggggattaaa
    19621 cacccctggc atatcgccca acgggcgtcg cgtcggcgcg gctatggcgt taactttcgg
    19681 ttcgttaatc aacatccgca gcaggtgacc gcccaccagc cccgttgcgc ctgtaatcag
    19741 tacctgactc atcttcgctc ctttacagaa ttgtctgcct tgcgctcccc ggctcatgca
    19801 ccaggcttaa tagtctacga ggtaattatc cccgatcgtg agaaattcgc ctcatccaat
    19861 gcaacaacgc ggaggaaaca tgagtaagaa gattgccgtt ttaatcactg atgaatttga
    19921 ggattcagaa tttacttcac ccgcagacga gttccgtaaa gccggacacg aagtgattac
    19981 cattgaaaaa caagcgggta aaacggtgaa agggaaaaaa ggagaaacca gcgtgaccat
    20041 cgataaatcc atcgatgaag tgacgccagc ggagtttgat gccctgctgc taccgggcgg
    20101 tcattcacca gattatctgc gtggtgacaa ccgttttgtc accttcacac gcgattttgt
    20161 gaacagcggc aaacctgttt tcgccatctg tcacggtccc cagttgctga tcagcgccga
    20221 tgtgatccgt ggtcgtaagc tgaccgccgt taaaccgatc gtgattgatg ttaaaaacgc
    20281 gggcgcggaa ttttacgatc aggaagtggt ggtcgataaa gatcagctgg ttaccagccg
    20341 gacaccggac gatctgccag cgtttaaccg cgaggcgtta cgcctgctcg gtgcctgagt
    20401 tacgtagcca aatcattttt ttaccaaagc ccagcgtgtt gtcggtgaat ttgatttcat
    20461 cgagccggat ttcccatacc ggggccgaca gcattctggc aaccggaaag cgacgattgt
    20521 acgccttgcg tgcgaggtcg ctttcctcgc cttccagtct gcggatctca cctttaaact
    20581 gcacgccgcg aattaacgct accgttttcg gctgaccgtt taccgttccg gcaattgccg
    20641 cctgcggccc gctcatctgc gcatgacgcg ttttttcttc cgtcaaaatg tagaaggcga
    20701 ctttctgcgc atcaaaaaga taaaaggcat tggcgcacca cagttcccct tcctgctgca
    20761 cacaccaggt gacaacatgt tgttttgcca gccaacggct gatggcggtg agtgtttcca
    20821 ttgctgttct cttttatact gtgggcctga ccttaacatg cgacgcagac gatgacaccc
    20881 tggtttcttt acttgatccg taccgccgac aataagcttt ataccgggat caccacggat
    20941 gtcgaacgcc gctatcagca gcaccaaagc ggcaaagggg cgaaagcact gcgcgggaag
    21001 ggagaactaa cgctggcgtt ttccgcacca gtgggcgatc gttcgctggc gttacgggca
    21061 gaatatcgcg ttaagcaact gaccaaacgg caaaaagagc gtctggtagc ggaaggtgca
    21121 gtgtttgcag aactgttgaa cagcctgcaa accccggaga ttaaaagcga ttgaagtgct
    21181 cgtgatactc aaccagacca gttacaccgt tcagcgcgtc atccgctaaa cgatgtacct
    21241 ggaaggcact ttcagtgccc ggccagcggc aacgcagatc gtgatgtgcc gccagttcaa
    21301 agccgaaacg gctgtacagc gccggatcgc ccagcgtcac cactgcggca tagccgaact
    21361 cattaagcga atcgagtcct tcatagacca gttggcgtgc cagcccctgt ccacggtatt
    21421 tttcatctac cgccaacggt gccatgccga cccattgcag gtcttcgccc tgcacatcaa
    21481 ccggactaaa tgccacatag ccaatgacct gaccttcgtc atctgtcgcc accagcccca
    21541 gcgtcagaaa gccatcttca cgcagatcgt gaaccagctt cgcttccgca tcactttcga
    21601 atgaacgacg cagcagggca tcaatacccg gcgcatcaat gggaatttct actcgaatta
    21661 gcatggttca cctaccgatg tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac
    21721 aaaatccgcc agttgcagca gcatcatgcg taaggctttc ggcatttgct ccagttcaat
    21781 ggcgtccatc aggtttttca catacagccc cagctccgta tcgccttcaa tcaccagccg
    21841 acgctggaag aagagcgtat ccggatcttg tttacgcgcc gcaatcatca gcagatcgct
    21901 ggcgtcggca ctaaaactca catcagcttg cgcgttctgg ctaacgacca gtttgccatt
    21961 caccaccgag gtaaaccatt gcaggtcaat atcacgcaca tgaatactta accagcgacc
    22021 ttcaagaaac tccagctcgc catcatccag cgcctgacgg aattgccagc ttaagacctg
    22081 ctcaagaacc tggtgtttta gcgcaaatgg cgtcagtttt accggtacac tcaacagaga
    22141 tggccccaaa tgcacaatac gggaacgcag tttatccaac acgagtttta ctccctgttt
    22201 caacaatcat cctattttgc catatcagaa aaataacata gcggtataaa tcaacaattc
    22261 catatgaaat tgctgctacc accaatacaa ctttaactgc cttaaatcaa aaattgtcgc
    22321 agcaaggtta actaaaatcc cagttcgtta acatttttgc gttttgataa cgcaaccttc
    22381 aggaaaaatt atggagctgc tctgccctgc cggaaatctc ccggcgctta aggcggccat
    22441 cgaaaacggc gcagatgctg tttatatcgg gctaaaagat gataccaatg cccgtcactt
    22501 cgccggcctt aactttaccg agaaaaaatt gcaggaagcg gtgagttttg tccatcaaca
    22561 tcgccgcaaa cttcacatcg cgattaacac ttttgcgcat ccggacggtt acgcccgctg
    22621 gcagcgcgcc gtggatatgg cggcgcaact gggtgccgac gcgctgatcc tcgccgacct
    22681 cgccatgctg gagtacgccg ccgagcgtta tccgcatatt gagcgccacg tatcggtgca
    22741 ggcttcggcg accaatgaag aggcgattaa cttttatcat cgccattttg acgttgcccg
    22801 cgtggtgctg ccgcgcgtgt tgtcgattca tcaggtgaaa caactggcac gggtcacacc
    22861 tgtaccactg gaagtctttg ctttcggcag cctgtgcatt atgtcggaag gtcgttgcta
    22921 tctgtcgtcg tatttgacgg gtgagtcgcc caacactgtg ggcgcgtgtt ctccggcccg
    22981 tttcgtgcgc tggcaacaaa cgccgcaggg gctggaatcc cgcctgaacg aagtgctgat
    23041 cgaccgttat caggacggcg aaaacgcagg ttatccgacg ctatgtaaag ggcgttatct
    23101 ggtggacggc gagcgctatc acgcgctgga agaaccaacc agtctcaata ccctggaact
    23161 gctgccggag ttaatggcgg cgaatattgc ttcggtgaaa attgaaggcc gccagcgtag
    23221 cccggcgtat gtcagccagg tggcgaaagt ctggcgtcag gctatcgacc gttgtaaggc
    23281 cgatccgcaa aacttcgtgc cgcaaagcgc gtggatggag acgctcgggt cgatgtccga
    23341 aggcacgcaa actacccttg gcgcgtatca ccgtaaatgg cagtgagaaa agcaatgaaa
    23401 tattccttag ggccggtgct atggtactgg ccaaaagaga cgctggaaga attttatcag
    23461 caggccgccg ccagcagcgc cgacgtgatt tatcttggtg aagcggtctg tagcaaacgc
    23521 cgggcaacca aagttggcga ctggctggag atggcaaaat cgctcgccgg aagtggcaag
    23581 cagattgtgc tctccacgct ggcgctggtg caggcatcct ccgaactggg cgaactgaaa
    23641 cgctatgttg agaacggtga gtttctgatt gaagccagcg atctcggcgt ggtgaatatg
    23701 tgcgctgaac gcaaactgcc gttcgtcgcc gggcacgcgc tgaactgcta caacgcggtg
    23761 acgctgaaaa tattgctcaa acagggcatg atgcgctggt gtatgccggt ggagctttcc
    23821 cgcgactggc tggtgaatct gcttaatcag tgcgatgagt tgggcattcg caaccagttt
    23881 gaagtggaag tcctgagcta cggtcatcta cctctggcct actccgcccg ctgctttacc
    23941 gcgcgttcgg aagaccgccc gaaagatgag tgtgaaacct gctgcattaa atatccgaac
    24001 gggcgcaacg tactgtcgca ggaaaaccaa caagtgtttg tactcaacgg cattcagacc
    24061 atgagcggct acgtttacaa cctcggtaac gagctggcat ccatgcaggg gctggtcgat
    24121 gtggtccgcc tgtcaccgca gggtaccgac actttcgcga tgctcgacgc cttccgcgct
    24181 aatgaaaatg gcgctgcgcc actaccgttg acggcaaaca gcgactgtaa cggctactgg
    24241 cggcggctgg ccgggctgga gctgcaagcg taagtatata gctcactttg ttaacaactt
    24301 taactactct ttaatgcagt attaaagact aatcggtaac aaagtgagct gttatgactg
    24361 ataaaaccat tgcgttttcg ctactcgatc tggcccccat ccccgaaggt tcttcagcgc
    24421 gggaagcgtt ctcccactct ctcgacctcg cccgtctggc tgaaaagcgc ggctatcatc
    24481 gctactggct ggcagaacac cacaatatga ccggcattgc cagtgctgca acgtcggtgt
    24541 taattggcta tctggcggcg aataccacca cgctgcatct ggggtctggc ggcgtgatgt
    24601 tgcctaacca ctcaccgttg gtgatcgccg aacaattcgg cacgctcaat acgctctatc
    24661 cggggcgaat cgatttgggg ctgggccgcg cgccgggtag cgatcagcga accatgatgg
    24721 cgctgcgtcg tcatatgagc ggcgatattg ataatttccc ccgcgatgtc gcggaactgg
    24781 tggactggtt tgacgcccgc gatcccaatc cgcatgtgcg cccggtacca ggctatggcg
    24841 agaaaatccc cgtgtggttg ttaggatcca gcctttacag cgcgcaactg gcggcgcagc
    24901 ttggtctgcc gtttgcgttt gcctcacact tcgcgccgga tatgttgttc caggcgctgc
    24961 atctttatcg cagcaacttc aaaccgtcgg cacggttgga gaaaccgtat gcgatggtgt
    25021 gcatcaatat tatcgccgcc gacagcaacc gcgatgccga attcctgttt acctcaatgc
    25081 agcaagcctt tgtgaaactg cgccgcggcg aaaccgggca actgccgcca ccgattcaaa
    25141 atatggatca gttctggtcg ccgtccgaac agtatggtgt gcagcaggcg ctgagtatgt
    25201 cgctggtggg cgataaagcg aaagtgcgtc atggcttgca gtcgatcctg cgcgaaaccg
    25261 acgccgatga gattatggtt aacgggcaga ttttcgacca ccaggcacgc ctgcattcgt
    25321 ttgagctggc gatggatgtg aaggaagagt tgttggggta gtgtgtttta acgtaggaag
    25381 ccttatccga gatggcaacg ctgtcctacg taggcctgat aagcgaagcg catcaggcat
    25441 tgtgtaggca gcagaaatgt cggataaggc accgctgatt actgatacac cggcagcaca
    25501 ttaaagctcg ataagatatg gaccagtgcg ttgccaacgc caaataccag aatcagcata
    25561 atcatcggct tgccgcccca tacacggaat ttcgggctac caaagcgttt acgcgatgcg
    25621 cgggccaaca gcgccggaac aattgccgcc cagatggtag ccgctaagcc agcataacca
    25681 atggcgtaca ggaatccgtt cgggaacagc aggccaccca caacaggcgg ggcaaaggtc
    25741 agcaacgctg ttttcaaacg gcccatagcc gagtcgtcaa aaccaaacag atctgccaga
    25801 tagtcaaaca aacccagcgt tacgccaagg aacgaactcg ctaccgcaaa gtttgagaac
    25861 acgaccagta gaagatccag actacggctg ttcagtacgc cgcttaacgc ctgtaccagc
    25921 acatcaatat taccgccctt ctcggcgata ccgataaact ccggacgcgg gatgttaccc
    25981 atcgtcgcca gcaaccagat ggtatacagc gccagcgcca tcagcgtacc gtacaccagg
    26041 catttcacga tggttttcgg atctttgccg taatacttca tcaggctcgg tacgttaccg
    26101 tgataaccaa acgatgccag acagaacggc agcgtcatca acagatacgg tgcataagac
    26161 gcattgcttt cggcgacgtt gaacaatgtc gcaggctgca catgccccag caggctgccg
    26221 aaggtgagga agaaagtaat gactttcgcc cccagcacaa ttgctgtcat ccggctgaca
    26281 gctttcgtac tcaaccacac cacgaacgct accagcagtg caaaaccaaa tccagctgcc
    26341 cgtgcaggga cgttcagcga catctccgcg aaggtgtgat gcagaatcga accgctggca
    26401 gaaatatagg cgtaagtcag gatatagagc acaaaggcaa tggaaatgcc gttgactacg
    26461 ttccagcctt tgcccagcaa atctttggtg atggtgtcaa agctagaacc gattcgataa
    26521 ttcaggttag cttccagaat catcaagccg gaatgcagca tacagaacca ggtaaagatc
    26581 agcgccgcca ttgaccagaa gaaccacgcc ccggacatga ccacaggcag agaaaacatc
    26641 cctgcgccaa taatggtgcc gccgataatc accacgccgc caagcagcga cggtgacgtt
    26701 tgggtggtgg ttagtgttgc catgagggct tctctccagt gaaaaatagt gcgactgtgt
    26761 tgttatgcat tgcactgtac cagtacatga gtacaaaaaa cagaaaaaaa gccccgatgg
    26821 ttaaaatcgg ggctgtatat attattttac agattgtgtt cgctgttcag cgatgattac
    26881 gcatcaccac cgaaacgacg acgaccggta gagtcatcac gacgcggtgc gcggccttca
    26941 cgacgttcgc cgctaaaacg acgcccatca ccacgacccc cttcacggcg ttcaccgctg
    27001 aagttacgac cgccttcacg acgttcgcca ccgaaaccac gaccaccgcc acgacgctca
    27061 ccgccagtat gcggctgtgc atcgcccagt aactgcatgt tcatcggctt gttgagaatg
    27121 cgagtacgcg taaagtgttg cagcacttca cccggcatac ctttcggcag ttcgatggtg
    27181 gagtgagaag caaacagctt gatgttaccg atgtaacggc tgctgatgtc gccttcgtta
    27241 gcaatcgcac caacgatatg acgaacttca acaccatcat cgcggcccac ttcaatacgg
    27301 tacaactgca tatcgccaac atcacgacgt tcgcgacgcg gacgatcttc acggtcgcca
    27361 cgcgggccac ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg gaattcgcgt
    27421 ttcggacgca tcggcgcatc tggcggaaca atcagagtac gttcaccctg tgccattttc
    27481 agtagtgcgg ctgccagagt ttcgagatcc agctcttcac cttcggcagt cggctgaatt
    27541 ttgctcagca gtgcacggta ttgatccaga tcgctgcttt ccagctgctg ctgtacttta
    27601 gcggcaaatt tttccagacg acgtttgccc agcaattctg cgttcggcag ttctacttcc
    27661 ggaatggtca gcttcatggt gcgttcaatg ttacgcagca gacgacgctc gcggttctca
    27721 acgaacagca gtgcgcggcc agcacgaccc gcacgaccgg tacgaccgat acggtgaacg
    27781 taagattcag aatccatcgg gatatcgtag ttaactacca ggctgatacg ctcaacgtcc
    27841 aggccacggg ccgcaacgtc ggtcgcaatc aggatatcca gacgaccatc tttcagacgt
    27901 tccagagtct gctcacgcag cgcctggttc atgtcaccgt tcagcgcggc gctgttgtag
    27961 ccgttacgct caagagcttc tgccacttcc agagtcgcgt ttttggtacg aacgaagata
    28021 atcgccgcat caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt tttacgcata
    28081 ccccagacag tccagtagct ctggctgatg tcaggacggg tagtcacgct ggactgaatg
    28141 cgcacttcct gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc ttccggcatg
    28201 gttgcagaga acagagcggt ctgatgacct tccgggatct gcgccataat ggtttcaacg
    28261 tcttcgatga agcccatgcg cagcatttcg tcagcttcat ccagaaccag accgctcagt
    28321 ttagagaggt ccagagtgcc acgtttcagg tggtccagca gacggcccgg agtaccgaca
    28381 acgatctgcg gcccctgacg cagggcgcgt aattgcacgt cataacgttg gccgccgtac
    28441 agggcaacca cgtttacgcc gcgcatgtgt ttagagaaat ccgtcattgc ttcagcaacc
    28501 tgtaccgcca gttcgcgggt cggtgccagc accagaatct gtggtgcttt cagctcagga
    28561 tcaagattct gcagcagagg taaagagaat gctgcagttt ttccgctccc cgtctgggcc
    28621 atacccagaa cgtcgcggcc attcagcaga tgtggaatac actctgcctg aattggagat
    28681 ggtttttcgt aacccagatc gttaagggct tcaaggatag gagccttcag gcccagatct
    28741 gcaaaagtgg tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa tggcggccag
    28801 tctacataac tcatcatgaa attgatcagc aattttcatt gaaaagtgtg aaccggctca
    28861 aagtaggtgt attaacgaac aacaacgccc tcacccgtta aggtgatggc aatcaaaaaa
    28921 gattacgggc tgatgtgtac gtcagctatt gctggtccga ttctgccagg tcatcttggt
    28981 cctggcccag gagcgataat tccaacaatg cgtatcggtg ctcaacaaag ttatgaacgt
    29041 tgttggcaac cgccagtttg aacagtgccg tggcgctgtc caaatccccc agacttaggt
    29101 agtacttacc taaatagaag ttggtttcac tgagatgctc agcgagcgag gtgttatccg
    29161 ttgcgtccgc cttgagcctt tccattaacg tttgttcgct aatgttgccc aggtagaact
    29221 cgacaatgtt ccatccccac tgttccttat ccgatttttc gaagtgctgt ttcaacactt
    29281 ctttagcctg cttctcatcg agcttctgct cggcgagata aagccacaga ctacggaaag
    29341 gatcattggg atcgtcttga taaaacgcca gcagatcatc ttgcgctaac ttgtcacgac
    29401 cgccgtaata taatgcgatc ccgcgattca agtgcgcgta gttgtaagtt ggatcaagct
    29461 caagtacaga atcaaacgct tcataggcag catcaaaatt gcctgcctgc gttaaatata
    29521 tgcctaagta attgaatact tcaggcatat ccggtcggat tgccagcgct tgcgaaaaat
    29581 cgttacgcgc taatgccctc agaccgagac tatcatacaa cactccgcgc tcatataaaa
    29641 gctgtgcgcg ttcgtcatcg gttaaagccc gactggcaag gatctgttcc atacgtgcca
    29701 gaatcacttc ctgctgtaaa gtcggttgca atggtaccgc gaggacttca cttttacgcc
    29761 aggaagtatt actgcatcct gcaagcgtaa gtgctgtcgc aatgaaacac cagcgcaaaa
    29821 aaggcttcat ttcccactcc cgaagaccac ggttgaatga acgtcctgtt cccggttgct
    29881 aacaaggcgt cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa tggcaacctt
    29941 actcgccctg ttcagcagcc ggagcttccg gtgctgcagc aggttgagac tgctcagtcg
    30001 cttctttaat gctcagacgg atacggccct ggcgatcaac ttccagcact ttcaccggta
    30061 cttcctgacc catctgcagg taatcggtca ctttctcaac gcgtttatca gcgatttgag
    30121 agatgtggac cagaccttct ttaccgccac caatggcaac aaatgcgcca aaatcaacga
    30181 tacgggtcac tttaccattg tagacgcggc ccacttcgat ttctgcagtg atctcttcga
    30241 tacgacgaat cgcgtgtttc gctttctcgc cgtcggtcgc tgcaatcttc acagtaccgt
    30301 catcttcaat ttcgatggtg gtgccggttt cttcggtcag agcacggatt acagaaccgc
    30361 ctttaccgat aacatctttg atcttgtccg ggttgatctt gatggtatgg atacgcggtg
    30421 cgaactcaga gatatcgcca cgcggcgcgt tgatcgcctg ttccattacc cccagtatat
    30481 gcagacgcgc acctttagcc tggttcagtg caacctgcat gatctctttg gtgatacctt
    30541 caattttgat atccatctgc aatgcagaga taccgtcacg ggaacccgca actttgaagt
    30601 ccatatcgcc caggtgatct tcgtcgccca aaatgtcaga cagtacaacg tagttgtcgc
    30661 cttctttcac cagacccatt gcgatacccg caacggcggc tttgattggc acacctgcgt
    30721 ccatcagcgc cagagacgcg ccgcacacgg aagccataga agaggaaccg ttggattcgg
    30781 tgatttcaga cacaacacgt acggtgtacg ggaatttgtc catatccggc attactgcca
    30841 gcacgccgcg cttcgccaga cgaccgtgac caatttcacg acgcttcgga gaaccgacca
    30901 tgccggtttc gcctacagag tacggaggga agttgtagtg gaacaggaag gtatcggtac
    30961 gttcgcccat cagttcatca agaacctgcg cgtcacgagc ggtacccagc gttgcggtaa
    31021 ccagcgcctg cgtttcacca cgggtgaaca gcgcagaacc gtgagtacgc ggcagcacgc
    31081 cagtacgcac atccagacca cggatcatat ctttttcacg accgtcgata cgcggttcgc
    31141 ctgccagtac gcggctacga acaacgtttt tctcaatagc gtgcagaatt tcacccagtt
    31201 cgttttcgtc cagggtttca tcttcagcaa gcagcgtcgc gatggtttca gatttgatga
    31261 catcaacctg cgcataacgc tcttgtttgt cggtgatgcg gtaagcatcg ctcagacgag
    31321 cttcagccag tgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc ggctgccagt
    31381 cccaacgcgg tttaccggct tctttcacca gttcattgat gttctgaata acaacctgct
    31441 gttgttcatg accgaacacc actgcgccca gcatttggtc ttcgctcagc agttcagctt
    31501 cagattcaac catcagcacg gcagcttctg taccggcaac aaccaggtcc agtttgctct
    31561 ctttcagttc gtcctgagtc gggttcagta cgtactggtc attgatgtaa cctacacggg
    31621 cagcaccaat cgggccattg aacggaatac cagacagaga cagtgctgcg gaagcaccaa
    31681 tcatcgcgac gatatccggg ttaacctgcg ggttaacaga aaccacggtg gcgataacct
    31741 gaacttcgtt gacgaagcct tccgggaaca gcgggcgaat cgggcggtca atcagacgcg
    31801 cgatcagggt ttcgccttcg cttgggcggc cttcacgacg gaagaagcta cccgggatac
    31861 gaccagcagc gtaggtacgc tcctgatagt taacggtcag cgggaagaag tcctgacctg
    31921 gtttggcttt tttctgacca acaacggtaa caaataccgc ggtgtcatcc atgctaacca
    31981 taacagcggc agtagcctga cgagccatca tgccggtttc cagagtcacg gtgtgttggc
    32041 cgtactggaa tttacgaacg atcggattaa gcaatgtaat atcctttctc tttcttagac
    32101 agtaccttac ggtactggtg ttaatacccg atcttctgcg catcctcgcg actaatgaca
    32161 accctaaccc agctctatgt gggtaaagcc tctcattagc cgcgcgaacc tctgcaacgg
    32221 aagatcattc atagcaacaa tacattagtt ttcagtgaat tgctgccgtc agcttgaaaa
    32281 aaggggccac tcaggccccc ttttctgaaa ctcgcaagaa ttagcgacgc agacccaggc
    32341 gctcgatgag ctgggtgtaa cgtgctacgt ctttacgttt caggtagtcg agcagtttac
    32401 gacgctgaga aaccatgcgc agcagaccac gacggctgtg gtgatctttt ttgtgctctg
    32461 caaagtggcc ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga acttcggtag
    32521 aaccggtgtc gtttgcgtca cgaccaaact cagaaacgat tttagctgtt gcttcagtac
    32581 ttagagacat tttaaaactc caaagtatat agaatgaaag gacgccgatc tctaattcag
    32641 cgatcccagt gtacgttacg caaagtgtta aacaatttac gcgacgttaa gcggcagtat
    32701 tctactcgta gcgacctgtt atcgcaagac ggttaacatt acgccgggta ttcaaccacc
    32761 aggcgacgag gcgcgacgcg gccttcatca tcaatttcgc ccataccgat aaacctgccg
    32821 ttctcacctt ctgtaacgcg aaccagtcct tccagcggcg caccggatgt acgaaccggg
    32881 ttaccatttt tgaagtaaac agaagacgtt aacggaagat tcaccaccgg gtagtccgaa
    32941 gctggactgt ccattggcat cagtaatgga tcaagtaact ccgcagccgg aatatcctgc
    33001 tgttcggctt gctcaaccag ttcacgcaga tgttccaggg tcaccatccg ttcaaccgga
    33061 tatttactta ccgccagacg acgcaagtaa ataacatgcg cgccacaacc gagtttttcg
    33121 ccgagatcgt caatgatagt gcggatataa gtgccttttg agcagtgaat ttccagctcc
    33181 agctcattgc cttcatggcg aataaacagc aattcataaa cggtaatcgg gcgcgcttca
    33241 cgcggaactt caatgccctg acgcgcgtat tcgtacagtt ttttgccctg atatttcagt
    33301 gctgaataca tcgaagggat ctgttcgata tcgccacgga aggtatccag cgccgccgcc
    33361 agttgctctg cactaaaggt taccggacgt tcttcaacga tttgtccgtc ggcatcagaa
    33421 gtatcggtac gctgtccaag acgcgcaatg acccgatagc gtttgtcgga gtccagcaga
    33481 tactgggaaa acttcgtcgc ttccccgagg caaattggca acatgccggt cgccagcggg
    33541 tccagcgcac cggtatgccc ggcacggttg gcgttataaa tccgtttcac cttttgcagc
    33601 gcatcgttgc tggacatccc ctgcggttta tccagcaaca aaacgccgtt aatgtcgcga
    33661 ccgcgacgac gaggacgact cattagtcct ccttgctgtc gtccgggtta acacgacgtt
    33721 cttcgtcatg tttgaccacg ctggtcacca ggtttgacat gcgcatccct tcaaccagag
    33781 agttgtcgta gaagaaggtc agttccggca cgatacgcag gcgcatcgct ttccccagca
    33841 ggctgcggat gaaaccagaa gcttcctgca acgctttgat gcccgcttta accgcgtctt
    33901 catctttgtc gttgaggaac gtcacatata ctttggcata cgccaggtcg cgagacattt
    33961 cgacaccgga aacggtggtc atcatgccca gacgaggatc tttaatttca cgctgcagga
    34021 tgagagcgat ctctttttgc atttcctgcg ctacgcgctg cgggcgacca aattctttcg
    34081 ccataataaa ttctcctgac aaaaaagggg ctgttagccc ctttttaaaa ttaatttcag
    34141 gtggaagggc tgttcacgtt ggcctgataa gacgcgccag cgtcgcatca gacaatccat
    34201 gcaggatgcg gcataaacgc cttatcccgc atggaaccct aaaaacctta agcgatggta
    34261 cgttggatct cgatgatttc gaatacttcg atcacatcgc cagtgcggac atcgttgtag
    34321 ttcttaacgc cgataccaca ttccataccg ttacggactt cgttaacgtc atctttgaag
    34381 cggcgcaggg actccagctc gccttcgtag ataaccacgt tgtcacgcag aacgcggatc
    34441 gggttgtgac gtttaaccac accttcggta accatacaac ctgcgatggc accaaatttc
    34501 ggtgatttga acacgtcacg aacttccgcc agaccgataa tctgctgttt cagttccgga
    34561 gacagcatac cgctcatcgc cgctttcact tcgtcgatca ggttatagat gacggagtag
    34621 taacgcagat ccaggctttc cgcttcaatc actttacgtg cagaggcatc agcacgtacg
    34681 ttgaagccaa ccaggatggc gttggacgcc gcagccaggg tagcgtcggt ttcggtgata
    34741 ccacctacgc cagaaccgat gatcttcact ttaacttcgt cagtagacag tttcagcaag
    34801 gagtcggaaa ttgcttccac agaaccctgt acgtctgcct tcaggacgat attcacttcg
    34861 tgaacttcgc cttcggtcat gttggcgaac atgttctcga gtttagattt ctgctgacgc
    34921 gccagtttaa cttcgcggaa tttaccctga cgataaagtg caacttcacg cgctttcttc
    34981 tcgtcacgta caacggtaac ttcatcaccc gcagccggta cgccggacag gccgaggatt
    35041 tccaccggaa tggacggacc cgcttccagc acttcctgac ccagttcgtt acgcattgca
    35101 cgaacacgac cgtattcgaa gccacacaga acgatatcgc ccttgtgcag agtaccttca
    35161 cgtaccagaa cggtagcaac cggaccacga cctttatcga ggaaggattc gataaccgca
    35221 ccgctcgcca tacctttacg taccgctttc agctccagaa cttccgcctg cagcaggata
    35281 gcatccagca gttcatcaat accggtaccc gcttttgcag atacgtgtac gaactggctt
    35341 tcgccgcccc actcttctgg caggatgccg tactgggaga gttcgttctt aacgcgatcc
    35401 ggatcggctt ctggtttatc gattttgttc acagcaacca ccaccggaac acccgccgct
    35461 ttcgcgtgct ggatagcttc gatggtctgc ggcattacac cgtcgtctgc agcaacaacc
    35521 aggactacga tgtccgttgc ctgcgcacca cgtgcacgca ttgaagtaaa cgcggcgtgc
    35581 cccggggtgt ccaggaaggt gatcatgccg ttttcagttt caacgtggta tgcaccaatg
    35641 tgctgggtaa tgccgcccgc ttcgccagag gccactttcg ttgaacgaat gtagtccagc
    35701 agagaggttt taccgtggtc aacgtgaccc atgatggtca caaccggagc gcgcggttca
    35761 gccgcagcac ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt ttcacgacgc
    35821 aggataactt tgtggcccat ctcttcagca accagctgtg cggtttcctg atcgataacc
    35881 tggttgatgg ttgccattgc gcccagtttc atcatcgctt tgatgacctg agagccttta
    35941 accgccatct tgttcgccag ttcgccaacg gtgatggttt caccgatcac aacgtcacgg
    36001 ttaacggcct gagcaggctt ctggaagcct tgctgcagcg aagaaccttt acgttttccg
    36061 cctttaccgc cacgtactgc tgcgcgtgct tcttcacgat cagcttttga ttcagcgtgt
    36121 ttgttgcctt tcttcggacg cgcagctttc gcgttacgac cacggccacg gccgccttcg
    36181 acttcacgat cgctttcgtc ttctgcctgg cgagcatgtt gagaagtagt gacgtgataa
    36241 tcgctggaat cttcagtcgg ttccgcgtta tcagtccatt tgttttcttc cgccatacga
    36301 cgtgcttctt cagcaacgcg acgtgcttct tcttcgagtt tacgacgcgc ttcttcttcg
    36361 gctttacgct tgagctctgc agcttcctgc tcacggcggg ctttttcagc ctgggcgttt
    36421 ttagtcatat cgtcttgttg attgctcact ttgtcttttt ccgcagcttc acgtttcgct
    36481 tgttcagcag cttcacgctt agcttgttct gcggcctcac gttcagcttt ttgttgcgcc
    36541 tcacgtttgg ccgattcttc tgcctcacga cgggcttgct cttccgcttc acgctgcgct
    36601 tgctcttccg cagcaaggcg ttcagcctct tgcggatcgc gtttcacaaa ggtgcgtttc
    36661 ttgcggactt cgatttgtac cgatttgctt tttccaccgg taccaggaat gttaagggtg
    36721 ctgcgtgttt tacgttgcag cgtcaatttg tccgggcctg aatttttctg attcaggtgg
    36781 tcaatcaaag tctgtttctc ttgtacagac acagagtcgt cagcagactt ccggatacct
    36841 gcatcagcaa attgctgtac caggcgttcc acggaggtct gtcgctctgc ggccagcgtt
    36901 ttaatcgtta catctgtcat gctgttcctt cctgctacag tttattacgc ttcgtcaccg
    36961 aaccagcaaa tattacgggc agccataatc agtgctccgg ctttttcgtc ggtcaaccct
    37021 tcgatatcag ccagatcatc aatgccctgt tcggcgagat cttccagcgt acaaacgcca
    37081 cgggcggcca gtttgaatgc caaatcacga tctacccctt caaggttcag cagatcgtca
    37141 gccggtttgt tatcaccgag gctttcttcc tgggcctgtg caatggtggc cagtgcattt
    37201 ttagcacgct cgcgcagtgc ttcaacggtc ggctcatcaa ggccttcgat ttccaacagc
    37261 tctttcatcg gcacataggc cagttcttcc agcgtcgaga agccttcttc taccagaaca
    37321 gtcgcgaagt cttcatcgat gtcgagatat ttggtgaagg tgtcgatcgc tgcgtgcgct
    37381 tccgcctgat gcttagcctg caggtcgtca acggtcatca cgttgagttc ccaaccgctc
    37441 agctgcgaag ccagacgcac gttctgaccg ttacggccaa tcgcctgcgc caggttaccg
    37501 gcttcaacgg cgatatccat ggtgtgttta tcttcatcca ccacgataga agcaacgtct
    37561 gccggtgcca ttgcgttaat cacaaactgc gccgggttat catcccacag gacaatatcg
    37621 atacgctcgc cgcccagttc agtagaaacc gcctgaacac gcgcgccacg catacctacg
    37681 caagcaccta ccggatcgat acgtttatcg ttggttttca ccgcaatttt cgcacgagat
    37741 cccgggtcgc gagccgctgc tttaatttca atcacttctt caccgatttc cggcacttca
    37801 atacggaaca gttcgatcag catttccggc ttggagcggg tgacgaacag ctgcgcgcca
    37861 cgcgcttccg ggcgaacgga atagagcacg ccacgaacgc ggtcgccagg gcggaagttt
    37921 tcacgcggca gcatatcttc gcgcaggatc acggcttcag cgttgttgcc caaatccaga
    37981 gagatgttgt cgcggtttac ttttttcacc acgccggtga tgatttcacc ttcgtgttca
    38041 cggaactgat caaccaccat cgcacgttcg gcttcacgca ctttctgcac gataacctgt
    38101 tttgccgtct gggtagtgat acggtcaaag gtaacagact caatctgatc ttcaacgtaa
    38161 tcgcccaggt tcaggctttc atcttcataa cgtgcggctt caagggtgat ttccttggtc
    38221 ggctgggtga cttcatcaac aactaaccag cgacggaagg agtcaaaatc accgcttttg
    38281 cgatcgatct gtacacggac gtcgatctct tgctcatatt ttttctttgt tgctgtcgcc
    38341 agcgcgcttt ccagtgcttc gaaaatcttc tcgcgaggta gcgccttttc attggatacg
    38401 gcttcaacta cagccaaaat ttctttgttc atcgcgggct tttcacctca tccagactat
    38461 taaaagtggg gaaccaggtt cgccttctgg atattactca gcgcgaacac ttcatcttta
    38521 ccttcgacgg taactgtgat catttcaccg tctaccgctt tgataacgcc ctgccattta
    38581 cgacggtttt gtaccgccat acggagaacc agagtcacct cttctccgac aaaacgggcg
    38641 tagtgttcag ccgtgaacag tgggcgatcg agacccggtg aggagacttc caggttataa
    38701 gcaacggtga tggggtcttc aacatccagc acagcactta cctggtggct cacatcagca
    38761 caatcatcaa cattgatgcc atcttcacta tcaatataga tgcgcagtgt ggatgtgcga
    38821 ccgcgaataa attcgatgcc aaccagttca aaacccaggg cctcaactgg cgcagtaatc
    38881 atctctgtta atttttgctc taatgtggac aagcccaccc ccaagacata aaaaaagggc
    38941 ctaaagccca gttattctgt agtcagataa caaaaaaccc cgataaatcg gggctttata
    39001 taactgaacc ctataaccgc aactgcggtc tggagcactt tccagaagga ttttttcaaa
    39061 tcccactacg aaggccgaag tcttcacagt atatttgaaa aagaactcta agggaaagtg
    39121 gttgcggggg ccggatttga accgacgacc ttcgggttat gagcccgacg agctaccagg
    39181 ctgctccacc ccgcgcctga aacgtggcaa attctactcg ttttgggtaa aaaatgcaaa
    39241 tactgctggg atttggtgta ccgagacggg acgtaaaatc tgcaggcatt atagtgatcc
    39301 acgccacatt ttgtcaacgt ttattgctaa tcacgtgaat gaatatccag ttcactttca
    39361 tttgttgaat acttttacct tctcctgctt tcccttaagc gcattatttt acaaaaaaca
    39421 cactaaactc ttcctgtctc cgataaaaga tgattaaatg aaaactcatt tattttgcat
    39481 aaaaattcag tgagagcaga aatccaggct catcatcagt taattaagca gggtgttatt
    39541 ttatgacgac gattctcaag catctcccgg taggtcaacg tattggtatc gctttttccg
    39601 gcggtctgga caccagtgcc gcactgctgt ggatgcgaca aaagggagct gttccttatg
    39661 catatactgc aaacctgggc cagccagacg aagaggatta tgatgcgatc cctcgtcgtg
    39721 ccatggaata cggcgcggag aacgcacgtc tgatcgactg ccgcaaacaa ctggtggccg
    39781 aaggtattgc cgctattcag tgtggcgcat ttcataacac cactggtgga ctgacctatt
    39841 tcaacacgac gccgctgggc cgcgccgtga ccggcaccat gctggttgct gctatgaaag
    39901 aagatggcgt gaatatctgg ggtgacggca gcacctataa aggaaacgat atcgaacgtt
    39961 tctaccgtta cggtctgctg accaatgctg aactgcagat ttacaaaccg tggcttgata
    40021 ctgactttat tgatgaactg ggtggccgtc atgagatgtc tgaatttatg attgcctgcg
    40081 gtttcgacta caaaatgtct gtcgaaaaag cttactccac ggactccaac atgcttggtg
    40141 caacgcatga agcgaaggat ctggaatacc tcaactccag cgtcaaaatc gtcaacccaa
    40201 ttatgggcgt gaagttttgg gatgagagcg tgaaaatccc ggcagaagaa gtcacagtac
    40261 gctttgagca aggtcatccg gtggcgctga acggtaaaac ctttagcgac gacgtagaaa
    40321 tgatgctgga agctaaccgc atcggcggtc gtcacggcct gggcatgagc gaccagattg
    40381 aaaaccgtat catcgaagca aaaagccgcg gcatttatga agcccctggg atggcactgc
    40441 tgcacattgc gtatgaacgt ttgttgaccg gtattcacaa cgaagacacc attgagcagt
    40501 atcacgcgca tggtcgtcag ttgggccgcc tgctgtacca gggacgttgg tttgattccc
    40561 aggcgctgat gctgcgtgac tctctgcaac gctgggttgc cagccagatc actggtgaag
    40621 ttactctgga gctgcgccgc gggaacgatt attcaatcct gaataccgtc tcagagaacc
    40681 tgacctacaa gccagagcgt ctgacgatgg aaaaaggcga ctcggtgttc tcgccagatg
    40741 atcgtatcgg tcaactgacc atgcgtaacc tggatatcac cgatacccgc gagaaacttt
    40801 tcggttatgc caaaactggc ctgctctcct cctctgccgc ttcaggggtg ccgcaggtgg
    40861 agaatctgga aaataaaggc cagtaattcg acatcaaccc tgcccttcag gggtggggtt
    40921 accttgatta tcctctgtaa actcctttct gagcatcaca taatcgaaaa catcatggtt
    40981 tgcatccaca acccgtgagt ctcctttata gtgtgcaatc acttcctcaa gcgtttgcgg
    41041 ctgttccggc tttgttaccc ccatccacgc attaataagg taattattgt aagctgtcga
    41101 aaagatgttg ttttccgttg tgcgatccac gccatcccct agaacggggc tataccagat
    41161 aaacatcggg acatgatacg cctgctggct agcctccctg cctccatgaa aatagacgtt
    41221 cttcttcgta gggtcgcgtt ccaggccgtg gtcggcaaaa tacatgaccg aggcgcggcg
    41281 atcttttaat aattcaaaaa cctgacctag caaactatct gtgtaatgaa tggagttgtc
    41341 atagcaggca tcctgatcgt cctgcggttg aaacacagcg ctggattgcg gataggcgct
    41401 acaagccggt tcatggcttc catttaaatg aagaacaatc agtttctttt gctgcgtctt
    41461 ttgctgtaac gcttgcgata aatgcggcaa caacaattca tcaaatcctc tgacatagac
    41521 tgtttccatg gcgcgcatgg cgatactggt aactgctgta ccattctgcc gaaaagcgga
    41581 ttgcgagctt agccagaaag tctgaaatcc tgcttgatta gccatattaa taatgttgtc
    41641 cgggtaatta tgaatgtcat gactcaaaac agaatcagca gttaaagaaa ggggaaccga
    41701 cagcgcagtg taaggtgcac cgcttattgc ttgattaaac agtttgatct gctttctttg
    41761 tgcttcgact tgcggtgtcg tagagcgtgt atatccatac aaagacatat tgtcgacacg
    41821 tacagactcc cccacaatca acacgtaggt atcaatacct gtatccctga ctgatagttg
    41881 aaaatacggc accgtgtttg caattgagag taatctttga tgctctttcg ctgctaaagc
    41941 aaaataattg agattgaaaa acggcgtata ggtagcaaat cgcgacgcta gtatatatgg
    42001 actgaacgcc tctttatttt ttgcatcttt ataagcaaat tgacaagcgg aaaataaact
    42061 gcccgaaatg acaatcagca ataatattcc tgtcactttt tttgtcggca aggaaacatc
    42121 atattttatt attactgcaa aaaaaagaag ggataaaaag gcaaaagcac atagataagg
    42181 gatatacatt cccagcattt tgacaacttc atccggatca ctctgcagca cactaatcgc
    42241 aaatccatca ttgaaagttg taccaaagat acaccatgaa tagaggctaa tactcatatc
    42301 tgccgcaatc agtaacacaa atggtacggc aataagcagg cgaagcggaa gggatgatat
    42361 acgtttaaca accaggatgg tcagaacgaa gaacagcaat cgctgaacca tatgcgctcc
    42421 ggaagaagcg actaagttcg taataccaaa aacaataaca caaagataca acaaccaatg
    42481 agatttaaaa cttttaacaa atttgttgaa tactgtcatc cagaaatcca agttcacaac
    42541 caatataaac aattatcaac aatgttcaat tttatatcag acatgttttt caactctgat
    42601 ccatatcaga ccgaaacact gttagatttt ttctttttaa atgatgcggc cattgcgttc
    42661 tcagtaatta aataacatta tcaatgcgtc tctaaacatt acgcagaacg tagccatcag
    42721 tagttatact gacaggggca ggatggttta tcgaggagta aacatatccg tattcgtacc
    42781 ggaaatggag ttgacacaat aaagtgccaa ttatgtcagc agaagggaaa agcttataac
    42841 taaggcatac cggcaccatc gtttcaaggt accagctacg agtaaaacaa ctggacgaga
    42901 tacagatacc tgacaaccat tcctcagacc aggaccaaaa cgaaaaaaga cgcttttcag
    42961 cgtctctttt ctggaatttg gtaccgagga cgggacttga acccgtaagc cctattgggc
    43021 actaccacct caaggtagcg tgtctaccaa ttccaccacc tcggcacgga tactactttt
    43081 tagttcggga tatcgctggt cggcttagcc ggagctgctg gctgagtttg ttcggttttc
    43141 gccggcgcac tcaggttttc ccattcgcta cctttattgg ttttgttgct attgatgtta
    43201 cccagcacca gactgatgat gaagaataac gttgccagca gcgctgtcat gcgggtcatg
    43261 aagttaccag aaccacttga accaaacagc gtagcggaag cgcctgctcc gaaggaggct
    43321 cccatatcag cgcctttacc ttgctgcagc atgatcagac caacaaggcc aattgccaca
    43381 ataaggaaaa ctactaaaag agcttcgtac ataatcaacc tgttccttgc ggagttgccg
    43441 cgtaccaatg cttcaaccaa taaagcggga gtttttaatc tttcccactg aagcgggtgt
    43501 gaatactaac caaagcgaat gaccttcgca agggcaattt tattgcattg tatcaactgc
    43561 ggaaaaaaac agcaaaaccg atgtgttcgc tgagaaaaaa ggcgacaatc gccgcctttt
    43621 tagccagcca tttatcgttt taaacagctt ttactgcatc ggcgatgcgg tgtgcaaatt
    43681 cagtcacctg cgcttcgtct tcgccttcca ccatcacgcg aattaacggt tcggtgccgg
    43741 atttacgcaa cagcacacgt ccacggctac ccagcgcagc ttcaacttct gcggtcacag
    43801 ctttgactga ctcatgctcc agcggatcgc cgctgcctgc tgtgtaacgc acgttaacca
    43861 gaatctgcgg gaacattttc atgccgctgc aaaggtcgtg caggctcata tggttacgcg
    43921 ccatcgccgc cagcacctgc aagccagcaa caatgccgtc accggtagtg gttttatcca
    43981 gcaggatcac atgaccggag ttctctgcac cgatacgcca gcctttctcc tgcatttttt
    44041 ccagtacgta ccggtcaccc actttcgcgc gcgcaaatgg aatacctaac tgtttcagcg
    44101 ccagttcaag ccccatgttg ctcatcaatg tacccacagc acctccacgc agctggccct
    44161 gacgaagacc ctcacgcgcg atgatataca tgatctgatc gccatcgact ttattgcctt
    44221 catggtcaac cataatcacg cgatcgccgt cgccgtcgaa ggcaataccg agatccgctt
    44281 tttcagccag cacacgagcc tggagcgcgc gaacgtcggt agcccccact tcggcattga
    44341 tatttacacc gttcggctca caaccgatag cgataacgtt cgcccccagt tcacgcagta
    44401 cgttcggcgc gatgtggtaa gtcgcaccgt ttgcacaatc caccacaatc gtcagttcac
    44461 tgaggctaag ttcgttcggg aacgtggctt tgcaaaactc gatataacga cccgcggcat
    44521 caacgatacg gctggcttta cccagttctg ccgaatcaac acagctgatc tctttttcca
    44581 tttccgcttc gatggcttct tcaactgcat ccggcagctt agtaccgtca atagaaaaga
    44641 atttaatgcc gttatcgtag aatgggttat gcgatgcaga aattacaatt ccagcctctg
    44701 cgcggaaggt acgcgtcaga taagccacgg ccggtgttgg catcgggcca gtgaagagcg
    44761 cggaaagacc ggcagccgca agacccgctt ccagtgctga ctccagcata tagccagaaa
    44821 tacgcgtgtc tttaccaata ataatcttac gggagccgtg gcgcgccagc actttacccg
    44881 cggcccaacc cagcttaagc acaaaatcag gtgtgatcgg cgcatcccct acacgaccac
    44941 gaatcccatc ggtaccgaaa tatttacgat tactcatagc gtttgttttc ctttgcagac
    45001 agagtggctt ccaccacccg catcgcttct acggtttctt tgacgtcatg aacacgaatg
    45061 atgtgcgcgc cttgcattgc ggcaatgacc gcacaggcca gactaccgct caggcgctcg
    45121 gacggcccca cgttcagcaa ttgcccaatc atcgattttc gtgacatacc caccaacagc
    45181 ggcaggttga aatggtgaaa ttcagccagg cgcgccagta atgaatagtt atgggagaga
    45241 tttttaccga aaccgaatcc ggggtcgagc aacaatttct cttttgcgat acccgcctgc
    45301 tcgcaacgtg ctatttgctc aataaagtag cgattcactt ctgcaaagac atcgtcatac
    45361 ttcggcgctt cctgcatggt ttttggattt ccctgcatat gcatcagaca aaccggtaaa
    45421 ccggtttctg cagccgcctc cagagcacca ggttcggaaa gggagcggat atcgttaata
    45481 atgtgagcgc caactttcgc tgactcacgg atgacttctg gtttggatgt atctaccgag
    45541 atccagactt cgaagcgttg agcaattgcc tcaaccacag gaataacacg ttgcaactct
    45601 tcttcaacgc taacttccgc cgcccctggg cgcgtggact cgccaccaac gtcaatgatc
    45661 gtcgcgccag cgttgatcat cagattcgca tgtttcaccg catctaacag cgagttatgc
    45721 gtgccaccat ccgaaaagga atcaggcgtg acgttgagga tccccattac gtgaggatgg
    45781 ctaaggtcca gtgaagtacc ctgggcaaag agtttcatga tgttatccct ggtatgaatt
    45841 gataagaaaa aaccccggag cacgccccgg ggttttcggt acaaatacag tcatctgata
    45901 cgggaactta cttgtcgcct aactgctctg acatggtgtt acccgggttc ggcgtacgcg
    45961 gttcatcaac cggacgagga gcctttggac taccattgtc gccagaattg ttagaagcgc
    46021 ctggttcttc ccagcccgct ggcggacgta catcgcgacg tgccatcagg tcatcaatct
    46081 gcggtgcgtc gatagtctca tatttcatga gagcatcttt catcgcatgc agaatatcca
    46141 tattgtcggt cagaagctgg cgcgcacgat tatagttacg ctcaatcagt gctttcactt
    46201 cctggtcgat gatacgtgca gtttcatcgg acatatgttt cgctttcgct acgctacgac
    46261 cgaggaacac ttcaccttct tcttccgcgt acagcagtgg acccaatttc tcagagaagc
    46321 cccactgggt caccatgtta cgtgccaggt tggtcgcaac tttaatgtcg ttggacgccc
    46381 cggtagaaac atgttccggt ccgtagatga tctcttccgc cagacgacca ccgtacagcg
    46441 tggagatctg actttccagt ttctgacggc tggcgctgat tgcatcacct tcaggcaaga
    46501 agaaggtcac acccagcgca cgaccgcgcg ggataatcgt tactttgtgc accggatcgt
    46561 gttccggtac caggcgaccg ataatcgcat gacccgcctc gtggtaagcc gtcgattctt
    46621 tctgcgcttc cgtcatcacc atggagcgac gttccgcacc catcatgatt ttgtctttcg
    46681 ctttctcgaa ctcaaccatc gaaacaacgc gtttgttgcc acgagcagcg aacagtgccg
    46741 cttcgttcac caggttcgcc aggtcagcac cggagaaacc aggagtacca cgggcaatga
    46801 ttgccgcgtc gatatcgggt gccaatggta cgcgacgcat gtgaactttc aggatctgct
    46861 cacgaccgcg aacatctggc aagccaacca caacctgacg gtcgaaacgg ccaggacgca
    46921 gcagggctgg gtcgagaacg tccggacggt tagtcgcagc gataacgatg ataccttcgt
    46981 taccttcaaa accatccatc tcaaccagca tctggttcag agtctgttca cgttcatcgt
    47041 gaccaccgcc caagcctgcg ccgcgctggc ggcctacggc gtcgatttca tcgataaaga
    47101 tgatgcacgg cgccgctttc ttcgcctgtt cgaacatgtc acgaacacgg gatgcaccca
    47161 caccgacgaa catttctacg aagtcagaac cggagatagt aaagaacgga actttcgctt
    47221 cgcctgcaat tgctttcgcc agcaacgttt taccggtacc cggaggacct accatcagga
    47281 cgcctttcgg gatcttaccg ccgagtttct ggaagcggct cggctcgcgc aaatactcaa
    47341 ccagttcagc aacttcttct tttgcttcgt cgcagcccgc aacgtcagca aaggtggttt
    47401 tgatctgatc ttccgtcagc atgcgcgctt tgctcttacc aaacgacatg gcacctttgc
    47461 caccgccgcc ctgcatttga cgcatgaaga agatccagac accaatcagc aacagcatcg
    47521 ggaaccagga gatgaagata gaagccagca ggcttggttc ttcaggcggt tcaccgacaa
    47581 ccttgacgtt cttggtcaac aggttatcca gtaatttcgg atcctgaacc ggaatgtaag
    47641 tggtataacg gttactatct ttcttggtaa cgttgatttc acgtccgttg atacgcgctt
    47701 cacgaacctg gtcgttattg acctcttgta ggaaggtaga gtaatccacc ttacggccat
    47761 tagactcgct gggcccaaag ctctggaata ctgacatcag cacaacggca atgaccagcc
    47821 agagtattag gtttttcgcc atgtcactca agggattaac ctcttattac aactgtgtta
    47881 aaaacagcgt caggatactc tatatccagc atctttcaaa ctttcgtctg aaatctcccg
    47941 gttagggttt acgcccggtc gctacaatat acacttcccg cgaacgtgca cgagaagagt
    48001 ccggcttacg aactttgacc ttcgtaaaca gggagcgaat ttcccttaga tactcatcga
    48061 aaccttcgcc ctggaacacc ttcactacaa aactgccacc tggcgctaat acatcacgac
    48121 acatttctag cgccagttcc accagataca tggcacgggg gatatccacc gccggtgttc
    48181 cgctcatgtt tggtgccata tcggacatga caacctggac tttgctgtcg ccaacgcgct
    48241 ccagcagtgc tttcatcacc agttcatcac gaaaatcgcc ctgaagaaag tccacaccaa
    48301 cgataggatc cataggtaaa agatcgcaag cgatgatgcg gcctttgccg ccgatttggg
    48361 tgaccacata ttgtgaccaa ccacccggag ctgcaccaag gtcgacaacc gtcattcccg
    48421 gtttaaagag tttgtcactt tgctgtattt catcaagttt aaaccaggca cgggaacgta
    48481 accccttttt ctgtgcctgt tgaacatatt tatcgctaaa gtgttcctga agccagcggc
    48541 tggagctggc agaacgcttc ttacctgtca tttaactttc ccatggggat aactcatcgt
    48601 aaccaattgc gtaaattttt acgcgcctat ttggtgatat atgggagatg gcggtagaat
    48661 gacccgtttt caatcccaac gtaagcaaaa aatacgatga atctgagtac taaacaaaaa
    48721 cagcacctga aaggtctggc acatccactc aagccagttg ttctgcttgg cagtaatggt
    48781 ttgaccgaag gggtgctggc cgagattgaa caagcgttag agcaccatga actcatcaag
    48841 gtgaaaatcg ccaccgaaga tcgcgaaact aaaaccttga tcgtggaagc tatcgtgcgc
    48901 gaaaccggcg cctgtaatgt acaggtcatc ggtaaaacgc tggtgcttta tcgcccaact
    48961 aaagaacgta aaatctcgct gccacgctaa gattatccta aagttacaca cattcgctgt
    49021 gtaaaacgag gggttttccg caggcaggag agcaaaatgc cacgctctgt tcgttgataa
    49081 aaggccgcat agcggccttt ttcctttctt tacaatacat caacatcttg agtattgggt
    49141 aattcttaca ggtattccac cttaattact tcaaattcta cttcgccgcc cggcgttttg
    49201 atgaccacaa catcatcttc ttctttgccg atcaggccac gagcaatagg cgagtttaca
    49261 gaaatcaggt tttgtttaaa gtcagcttcg tcatcgccaa cgatgcgata agtctgttct
    49321 tcgtcagaat cgagattcag caccgttacg gtagcaccaa aaataacgcg cccattgttg
    49381 ggcattttgg tgacatcaat cacctgcgcg ttcgacagct tggcttcgat gtctttgata
    49441 cggccttcgc agaaaccctg ctgctcacga gctgcgtggt attcggcgtt ttctttcagg
    49501 tcgccatgct cacgcgcttc cgcgatagca gcaatgattt caggacggcg cacagatttc
    49561 agaaaatcca gctcttcgcg taatttttca gcgccgcgta aggtcatcgg aatagcttgc
    49621 atttgttata cctcttgaat attcctgatg gggcaagtct tcacccaccc cggctgttcg
    49681 gcctgcccgg cataactcct ctccgggacc agaagcaaaa aaataccgac ccgggtacaa
    49741 gtcccaggtc agctacaatt cacattttga tagtcatttt accctggagt tcccgaaggg
    49801 tcatcgttta ctttataggg cgttgcgccg tagtatgacg gctcgattcc aggttgttag
    49861 cgcgagatta tgcgattttc cagatttatc atcggattga ccagctgtat agcgttcagt
    49921 gttcaggccg caaatgttga tgagtacatt actcaactcc ccgcaggtgc caaccttgcc
    49981 ctgatggtgc aaaaagtcgg cgcgtcggcc cccgctattg attaccacag tcagcagatg
    50041 gcgctacccg ccagtacgca gaaagtgatt actgcgctgg cggcgttgat tcaactcggc
    50101 cctgattttc gttttaccac gacgcttgaa accaaaggca atgtggaaaa cggcgtactt
    50161 aagggtgact tagtagcgcg atttggtgcc gatccgacgt taaaacgtca ggatattcgc
    50221 aatatggtca cgactttgaa aaaatctggc gtcaaccaaa tcgatggcaa tgtgttgata
    50281 gatacctcca ttttcgccag ccacgataaa gctcccggct ggccatggaa tgacatgaca
    50341 caatgcttta gcgctccgcc tgccgccgcc atagttgacc gcaactgttt ctccgtctcg
    50401 ctctacagtg ccccaaaacc tggtgatatg gcttttatac gcgtggcatc ttattacccc
    50461 gttacgatgt tcagtcaggt acgcaccctc ccccgtggtt ctgccgaagc gcaatactgc
    50521 gaactggatg tcgtaccagg tgacctgaac cgctttacgc taacgggatg cttgccacaa
    50581 cgttctgagc cgctcccgtt ggcttttgcc gtgcaggatg gtgccagcta tgccggggca
    50641 attctgaaag atgagttaaa acaggcgggt atcacctgga gcggaacgct gctgcgccag
    50701 actcaggtta acgaacctgg aacggtagtt gccagtaaac agtcggcccc gctgcacgat
    50761 ctgcttaaga ttatgctgaa aaagtcggac aacatgattg ccgatacggt tttccgcatg
    50821 atcggccatg cgcgcttcaa tgtgcctgga acatggcggg ccggatcgga cgccgtgcgt
    50881 cagatcctgc gccagcaagc cggtgtcgat atcggaaaca ccattattgc cgatggttca
    50941 gggctttcgc ggcataacct gattgccccc gccaccatga tgcaggtgct gcaatatatt
    51001 gcccaacacg acaatgaact taactttatc tccatgctgc cgctggcggg ctatgacggc
    51061 tctttgcagt accgtgcagg tctgcatcag gcgggcgtgg atggaaaagt ctcagcgaaa
    51121 accggttcgt tgcagggggt atataacctg gcgggattca ttaccacagc gagcgggcaa
    51181 cgaatggcgt ttgtgcaata tctttctggc tatgcagtag aaccggcgga tcagcgtaat
    51241 cgccgtattc cgttagtgcg ttttgaaagc cgtttgtata aagatattta tcagaataat
    51301 tagcctaaag agaaaccccg gcacatggcc ggggttttgc tgattaacgc ttgtaaatga
    51361 actcaacgcc ttcttcgtcg tcttcgtccc agtcgtcatc ccagtcctca tcatcctctt
    51421 cagcaatctc ttcaagctgc tgacggtgat aatcatccca catgaattcg actttctctg
    51481 gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat gataaaggtc atcacatccc
    51541 agcagagatc tttcacgccc agtccgctcg ccgcagagat cagataatat ttatcttccc
    51601 agcccagcgc ttcagcgatc gctttcgctt tctcttcggc ttctacctta tccagcagat
    51661 cgatcttgtt gaagactaac caacgcggtt tcgccgccag atcctggctg tatttttcca
    51721 gctcactgat aataatacgc gcgttttcaa ccggatcggt gccgtcaatc ggatcgatat
    51781 cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt caggaagcga atgcccagac
    51841 ctgcgccttc tgcagcgcct tcaatcagtc ctggaatatc ggcaacaacg aagctctttt
    51901 cgttgtccat acgcaccacg cccaggcttg gtaccagagt ggtaaacgga taatccgcca
    51961 ctttcggttt agccgccgat accgcacgaa taaaggtcga tttacccgcg tttggcatcc
    52021 ccaacatacc gacgtcagcc agcagcatca gctccagcag cagctcacgc ttatcgcccg
    52081 gcgtaccgtt agttttctgc cgtggtgtac ggttaactga cgacttgaaa cgggtattgc
    52141 ccagaccgtg ccagccgccc ttagcaacca gcagacgctg accgtgtttg gtcatatcgc
    52201 ccatggtttc accggtaccc tggtcgataa cacgcgtacc tacaggcact ttaatcgtca
    52261 cgtctttacc gcgcttaccg gtacagtcgc ggcttgcgcc attctgacca cgctctgcgc
    52321 ggaaagattt ttcaaaacga taatcgataa gcgtgttcag gttctcgtcg gcttccatcc
    52381 atacgtcacc gccgtcaccg ccgtcgccgc catccgggcc gcctttcgga atatactttt
    52441 cgcggcggaa gctcacgcaa ccattaccgc catcacctgc aacgaccaga atcgatgctt
    52501 catcaacaaa cttcatttta ttctccgtaa atcattcgcc tgcgcggggt tgcgatacca
    52561 ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc aatggcggaa tacatcgcgc
    52621 ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt taacatcggt ctggcgaaga
    52681 aatcgggcca ggccagtgat aaaagatctg aaaaaaacag cgtaaacagt ggggtgagcg
    52741 tgatgatcgc gctcacctgc gctgcctgcc agcgagccat cgcttccgcc agggcgccat
    52801 atcctaccaa tgtattcagt ccgcaaaaaa ttaaacatgc gagctgccag tggctaagct
    52861 gcgctatcac tccaggattt gccagaggga agagcgcaat tgtacataaa gtgtacaaca
    52921 aaaacaggat ctgcggtgaa gccagccgac gcaataaaac cttttgcgcc acgccataac
    52981 tcacccaaac cgtcgccgca ccgaccccaa agataactcc ccaggtgtaa tcggtgagct
    53041 ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac caggccgctc aggagcatca
    53101 atgcccctac aacctgagta ctgcgcattt tctctttcag gataaatacg ctggcaacca
    53161 tcatgccaac tggcgagagt tgcccaatca cctgcgaagc ggtcggactc aggtattgca
    53221 aggatgagct gaacaggatg aagttcccaa acagcccggc ggtcgccact gctaacaaaa
    53281 tcaaccagcg tggcttacga aacacgcgta atggcggcaa ccgcttcttc accgcaagaa
    53341 tggcaccaag gccaatactc gccatcaaga aacggtaaaa cacgattgtc ggaggttcca
    53401 tcacctccag cacctgcttc attgcgattg gcaacgcccc ccagcaaatt gctgtggtga
    53461 gcgccaaaag aatgccaatg cctgcctgct gcttcatacc gtattccctg caaagcgcat
    53521 atgcggatta acgtcgcacc atgcgcattt ttaccggtta tcgaatgtaa aaagccccgc
    53581 aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa aacttattca gcttcgatgc
    53641 tgataaattt acggtttttc gggcctttaa cttcgaattt cactttaccg tctgctttag
    53701 caaacagagt gtggtcacga ccgcaaccta cgttagcgcc agcgtggaat ttggtaccac
    53761 gttgacgaac gatgatgcta cccgccagaa cggattcgcc accgaaacgc ttaacgccca
    53821 ggcgtttagc ttctgaatcg cgaccgttac gtgtggagcc gccagccttt ttatgtgcca
    53881 tttgaaatct ctcctcaggt cttaggcgct gatgccagta attttcacat cagtgaacca
    53941 ctgacgatgg ccctgctgct tacgatagtg tttacgacga cgaaacttaa cgattttaac
    54001 tttctcgcca cgaccgtgag caacaacttc agctttgatt acgccgccat caacgaaagg
    54061 aacgccgatt ttgacttctt caccgtttgc gatcatcagc acttcagcga actcaacagt
    54121 ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc tgaccttcgc ttactcggtg
    54181 ttgtttacca ccactttgga aaaccgcgta catataaaac tccgcttccg cgcacacctt
    54241 ttcaatgatt cagagtgcgc tataaatatt cacaataggg cgcgaatatt acgcaaaacg
    54301 cacgcctttg acaagtgcta cagtcaatac acgaagaaaa aaaacacaac ttgtacggta
    54361 acgtttatct gtgccatttt ttcagtacaa tcaccctata ttcctaacca taaaccctaa
    54421 gttgcctttg ttcacagtaa ggtaatcggg gcgaaaagcc cggcttttgc gatgaattta
    54481 gaaaaaatca atgagttaac cgcgcaagat atggcgggtg ttaatgcggc aatccttgag
    54541 cagcttaatt ccgacgtcca actgatcaat cagttaggct attacatcgt cagcggcggc
    54601 ggtaaacgta ttcgtccgat gattgctgta ctggctgcac gagctgttgg ctatgaggga
    54661 aatgcgcatg tcaccattgc tgccctgatc gagtttatcc acacggcgac tctgctacac
    54721 gacgacgttg tggatgaatc agatatgcgc aggggtaaag ctaccgccaa cgccgcattt
    54781 ggcaatgccg ccagcgtgct ggtaggcgat tttatttata cccgcgcgtt ccagatgatg
    54841 accagcctcg gctcgctcaa agtgctggaa gtcatgtcag aagccgtaaa cgtcatcgca
    54901 gaaggtgaag ttctgcaact gatgaacgtt aacgatccgg acatcactga agaaaactac
    54961 atgcgcgtta tctacagcaa gactgcgcgt ctgtttgagg ctgccgcgca gtgttccggg
    55021 attctggctg gctgtacgcc ggaggaggaa aaaggcctac aggattatgg gcgctatctc
    55081 ggcactgctt tccagttgat cgacgattta ctcgattaca atgccgatgg cgaacagtta
    55141 ggtaaaaacg tcggcgacga tctgaacgaa ggtaaaccga cgctgccgct gctgcatgcg
    55201 atgcatcatg gcacaccaga acaggcacag atgattcgta ccgccatcga acagggtaac
    55261 ggtcgccatc ttctggaacc ggttctggaa gcaatgaacg cttgtggatc tcttgaatgg
    55321 acgcgtcagc gtgccgagga agaagcagac aaagccatcg cagcgttaca ggtgctcccg
    55381 gacaccccgt ggagagaagc acttatcggc ctcgcgcaca tcgctgttca acgcgatcgt
    55441 taatcccctc ccctcatccc gcgcagtgcg cgggatgatt ccagtaaatt ccataaaaac
    55501 acttattcag ctctataaat cctgaaaaac agggctgtgt aatctttata tattctgaat
    55561 attcacgctc tttacaggaa ctttttagag caataggcca tcaggagtat agtgatgctc
    55621 gacagaaaca gaagaagtgt tctgaatgaa agcgactaac ttaaggagtg aggaaagatg
    55681 gaaagtaatt tcattgactg gcatcccgct gacatcattg caggtttgcg caagaaggga
    55741 acgtcaatgg cggcggagtc tcgcagaaat ggtttgagtt cctcaacgct ggcgaacgca
    55801 ttatcacgcc catggccgaa aggagagatg attattgcga aagccctggg aactgacccc
    55861 tgggttatct ggccatcacg ctaccatgat ccgcagaccc atgagtttat cgacagaacg
    55921 cagttgatgc gtagctacac taaaccgaaa aaatgagtgg tctggcggta gccccgcgaa
    55981 cggggctgcc agctctcaga caattactcg cctttcacac gctcaatatt tgcacctaaa
    56041 gcgcgtagtt tgtcttcaat gcgttcgtag ccacgatcga tgtgataaat acgatcaacc
    56101 accgtcgtcc cttccgcaat acagccagcc agcaccaggc ttgctgatgc acgcagatcg
    56161 gttgccataa cctgtgcgcc agaaagtttt tcaacaccgt gacaaataac ggtattgctt
    56221 tcgatttcgg cgtgcgcgcc catacggctc agctctggca catgcataaa gcggttttca
    56281 aagaccgttt cggtgatgaa tccggtccct tctgccacca ggttcaacag cgtgaactgg
    56341 gcctgcatat cggtcgggaa tgccggatgc ggcgcggtac gtacgttaac agccttcgga
    56401 cgtttgccat gcatatccag gctaatccag tcttcgccga cttcgatgtc cgctccagcg
    56461 tcacgcagtt tcgccagcac ggcgtccaga gtatctggct gcgcgttacg gcagataatt
    56521 ttgccgcgag agatcgccgc cgccaccagg aaagtaccgg tttcgatacg atccggcagc
    56581 acacgataga caccgccgcc taaacgttcc acaccttcga tgacgatacg atcggtgccc
    56641 tgaccgctaa ttttcgcacc cagcgtaatc aggaagttcg cggtatcgac gatttccggt
    56701 tcacgcgctg cgttttcaat aatcgtggtg ccttccgcaa gggttgcagc acacatgatg
    56761 gtcaccgttg cgccaacgct gactttatcc atcacgatat gtgcgccttt caaacgacca
    56821 tcgacggaag ctttaacgta accttcttcc agtttgatgg tcgcgcctaa ttgttcgagg
    56881 ccagaaatgt gtagatcaac cggacgtgcg ccgatcgtac aaccgccagg cagtgaaact
    56941 tgcccctgac caaagcgcgc taccagcggc cccagcgccc agatagaagc acgcatggtt
    57001 ttaaccagat cgtaaggtgc gcagaataca ttaacgtcgc gggcatcaat atgcacagaa
    57061 ccattacgtt ctactttcgc acccagctgg cttagcagct tcattgatgt atcgacgtct
    57121 tttagtttcg ggacgttctg gatctctacc ggttcttccg ccagtagtgc ggcaaaaagg
    57181 ataggcagag cagcattttt agcgccggaa attgtgactt cgccctggag cttcgttggc
    57241 ccctgaacac gaaatttatc catttagttt gttctcagtt aacaattcat atccgctacc
    57301 ggcgaatcgc ccatagctca aaagccgttc agtttgcgat cgcgcgccca ctccgcaggg
    57361 gtatacgctt tgatcgacac agcatgaatg cggttatccg caatatattc catcagcggg
    57421 ccatagaccg tctgctgttt tttaacccga ctcatgccgt caaacaactc acccacggca
    57481 ataacctgaa agtggctgcc atcgccggaa acgtggactt cctggaggga gagagcgttc
    57541 atcaacacgc tctgaatttc attattttcc atgggatctt caatcatcag ttaataaatc
    57601 agcgaaacat cttagagcaa agttgcgctg gcataaataa gcaaaaagcc tcgctgataa
    57661 atcagacaag gctcgacttg caggcaggtt tgccggacag gcggttaacg ccatatccgg
    57721 cctgaaaaaa tttaacgagg cagaacatca gcaggcaaat tatacaattt cgccagggta
    57781 tacactttgt cgtttacccc ctgaagcgtc acattgttgc cctgcttttt cgccagatcg
    57841 ataagatgga gcagcagtgc cagtccaccc gtatccacgc gagacacacg gctaagatcg
    57901 atgctggtaa tccccttcac cgcttcctca cgtatttccc aaagcggtag cagaacgtcc
    57961 tgatccagct ctccggataa cgccagcgtg tcacccgtct gcatccagct cagtgactcg
    58021 ctcattattt tttctcttcc agagtgattt tctgttgaga aatcgatttc agttgcgcag
    58081 tcaggccgtc gatacctttg gtacgcagca gcgttcccca ctcgttttgt ttggtggtga
    58141 tcatactgac gccttcagca atcatgtcgt aagcctgcca gttgcccgtc tgggagtttt
    58201 tacgccactg gaagtccaga cgcaccggcg ggcggccatt cgggtcaata atggtaacgc
    58261 gaatcggcac aatggtttta tcacccagcg gctgttctgg cgcaatctga taggtttgac
    58321 cgtgatacat cgccagcgcc tgaccgtaag cctgcttcag gtactcacgg aaagcggcaa
    58381 agtaagcatc acgttgagct ggggtcgcac tcttgtaata ctggcccagc accagcgcac
    58441 cggcgtattt cacctgtacg tatggcaaca gttcctgatc aacaatggta cgcagataat
    58501 ccgggttagc ccgaatttgc ggttgctcat tcttcaggcg atcgaacgtt ttctgcgccg
    58561 cctcgtccat cagcttatac ggattggtct ggtctgccgc ggttgccgca ctcagcggtg
    58621 caatcaccag caaagcgacc atcattaaac gtttaaacat gcgtcggttc tcctgaaatt
    58681 atttcgttgt acccacaggt tcagtggttt cattattacc tggcgcagca gctggcgcat
    58741 cgccactatt cttattgtca tcgcctttac taccgtaaag gaactgacca atgagatctt
    58801 ccagcaccat cgcagacttg gtgtcctgaa ttgtatcgcc atccttcagg atagcagttc
    58861 ccagttccgg gtcttcaaaa ccgacgttta atgccagata ttgttccccc agcaggccgg
    58921 aagtacgaat gctcagcgaa ctggtatcag gaatgtggtt ataacgttgt tcaatttcca
    58981 gcgttacgcg cggcagatag gttttcgggt ccagcgtaat atccgccacc cggcccacaa
    59041 caacgccacc aatgctgacc ggagagcggg ctttcaggcc gccaatgtta tcgaacgtcg
    59101 cataaagcgt ataggtcggt tcagtacgta tagacgtcac gttcgctgcc ttcaggcaaa
    59161 caaacagcgc cgcaagcagt gctgctaata aaaagatacc cacccaaatt tcattttttt
    59221 tcgtttgcat gaactcaatt cccaaacatc aatgcggtca gcacaaaatc cagccccaga
    59281 acagccagag acgagtggac aacggtgcga gtggttgccc ggctaatccc ggcagacgtc
    59341 gggatggcgt cgtagccgtt aaacaacgaa atccacgtca ccgtgatggc gaacaccacg
    59401 ctcttaatca gacagttgac gagatccata cgccagtcga cggcattttg cattgccgac
    59461 cagaagaacc cactatcaat gcctttccaa ctgacgccga ccagcgatcc gccccagatc
    59521 cccacggcga cgaagataac cgtcaacagt ggtaatgaaa taactccagc ccagaaacgg
    59581 ggagaaataa tccgacgcag cggatccacc gccatcatct ccatactgga gagttgctct
    59641 gtagcgcgca tcaggccgat ttctgcggtt agcgccgaac cagcacgccc ggcaaacaac
    59701 aacgcggcaa ccaccggccc cagttcacgc agtagcgata acgccaccag catacccaga
    59761 ctggtttccg cactataagt ggtcagaacc agataacctt gcagccccaa caccattccg
    59821 atgaacacgc cagaaacaac aataatcagc atcgacagga cgccgacatt atagagctgg
    59881 cgcaccagca gcggtgcatg tttgcgaaat tccggtttac cgaccagcgc attgaataac
    59941 attaacccgg cccgcccgaa cgttctcagg gttttaatcc ctttatgtcc gagcgacgcc
    60001 agcgcattta acagcatgag tggcttaact ccctggtaaa agatcagcgt gataatcgcc
    60061 ggcaggatag cggaacggaa caggcccgtc agctatcccg tccagaaact gacgtacgcg
    60121 cggatcagga ttcgcctgca acgcctgggc actgccatga gcgacaattt ttttgtccgc
    60181 caggatccag gcgtgatccg caatacttaa cacttccggc acatcgtgag aaaccaccac
    60241 acaagtcacg cccagcgcgc tgttcagctc agaaatcagc ttcaccagca cgcccatggt
    60301 aatgggatct tgcccaacaa agggctcatc aaacatgatg agatccggct ccagcgcaat
    60361 cgcacgtgcc agcgctgcac gccgcgccat cccaccggaa agttcagaag gcataagttt
    60421 agccgctcca cgcagcccca cggcctccag cttcatcatc accgtactat gcaacaatgg
    60481 cgcgggaagt tgggtatgtt cccgcagtgg ataggcgacg ttgtcaaata cgttcatatc
    60541 agtgaacaac gccccggact gaaataacat gctcatccgt ttgcgcactg tatacaggcg
    60601 cgaacgagac atcgccggaa tattctcacc atcgaaaagg atctcaccat gatctggtgc
    60661 gatttgcccg ccaatcagac ggagtagcgt cgttttaccg atgcccgatg gccccatgat
    60721 cgccgtgatc ttccctcgcg gcacggtcag ggaaatatta tcgaagatgc agcgattgcc
    60781 acgcgtaaaa ctgacatcgc gcatatcgac taaattcgcc acagactgct ccataattca
    60841 cccttcgtct tgcgttgatt ttctaagcat ggcgctcaat ttaaccttga acccaacata
    60901 tttacagaat attacctgcc gtggttagcg aaagctggca tttgttttac tttttagccg
    60961 cataaagtca aaattaagaa tctgttacgg ctttctgaaa atcttcagcg gaccggcgag
    61021 tatacctgaa gaaaggacgt tagatgcttt tagctacggc actgttaatt gttggtttac
    61081 ttttggtcgt ttacagtgcc gaccgcctgg tttttgccgc gtctattctt tgccgaacct
    61141 ttggcatccc gccgctgatc atcggcatga cggtggtcag tattggtaca tcgttaccag
    61201 aaatcatcgt ctcgcttgcc gcgtctttgc acgaacaacg cgatttagcc gttggtacag
    61261 ccctcggctc aaacattatc aatatattgc tgatcctcgg tctggctgcg ctggttcgtc
    61321 cttttaccgt ccattctgat gttctacgcc gtgaattacc cttaatgttg ttggtcagcg
    61381 tggtggccgg ttccgtactc tatgacggac aacttagtcg cagcgatggt atctttctcc
    61441 tctttctggc tgtgctatgg ctgctgttca ttgttaaact tgcacgtcag gctgaacgtc
    61501 aggggactga cagccttact agagagcagc ttgcagagct gccgcgtgaa ggtggattgc
    61561 ccgtcgcatt tttatggctc ggcatcgcac tgatcattat gccagtggcc acgcggatgg
    61621 tagttgataa cgccacggtg ctggcgaatt actttgccat tagcgagttg acgatgggtc
    61681 tgacggcaat tgctatcgga accagcctgc cggaactggc aaccgcaata gcgggggttc
    61741 gcaaaggtga aaacgacatt gcggtcggaa atatcattgg cgcaaacatt tttaatattg
    61801 tcatcgtgtt gggtttaccc gcgctgataa cgccaggaga gattgatcca ctggcgtaca
    61861 gtcgtgacta cagcgtgatg ttgctggtga gcattatttt tgcgttgctg tgctggcggc
    61921 gctccccgca accgggccgt ggtgtagggg tattattaac tggcggattt atcgtatggc
    61981 tggcgatgtt gtactggtta tcgccaatac tcgttgaata actggaaacg cattatgtcg
    62041 cacgtagagt tacaaccggg ttttgacttt cagcaagcag gtaaagaagt cctggcgatt
    62101 gaacgtgaat gcctggcgga gcttgatcaa tacatcaatc agaatttcac gcttgcctgt
    62161 gaaaagatgt tctggtgtaa agggaaagtt gtcgtcatgg ggatggggaa atcggggcac
    62221 atcgggcgca aaatggcagc aacatttgcc agcaccggta caccttcatt tttcgtccat
    62281 cctggcgaag ccgcgcatgg tgatttaggc atggtcaccc cacaggatgt ggtgattgct
    62341 atctctaact caggtgaatc cagcgaaatc acggccttaa ttccagtgct taagcgtctt
    62401 cacgtaccgt taatctgcat caccggtcgc ccggagagca gcatggcgcg cgccgcagat
    62461 gtgcatctgt gtgttaaagt agcgaaagaa gcctgtccgt tagggctggc accgaccagc
    62521 agcacaaccg ccacgctggt tatgggcgat gccctcgctg tcgcgctgtt aaaagcacgc
    62581 ggctttactg ctgaagattt tgcgctctca cacccaggcg gcgcactggg tcgtaaactt
    62641 ctgctgcgcg taaacgatat tatgcatacg ggcgatgaga tcccgcatgt taagaaaacg
    62701 gccagtctgc gtgacgcatt gctagaagtt acccgcaaaa atcttggtat gactgtcatt
    62761 tgcgatgaca atatgatgat tgaaggcatc tttaccgacg gtgatttacg ccgtgtcttc
    62821 gatatgggcg tggatgttcg tcagttaagt attgccgatg tgatgacgcc ggggggaata
    62881 cgcgtgcgcc caggcattct ggccgttgag gcactgaact taatgcagtc ccgccatatc
    62941 acctccgtga tggttgccga tggcgaccat ttactcggtg tgttacatat gcatgattta
    63001 ctgcgtgcag gcgtagtgta aagattcaag gataaacaac aatgagcaaa gcaggtgcgt
    63061 cgcttgcgac ctgttacggc cctgtcagcg ccgacgttat ggcaaaagca gagaacattc
    63121 gtctgctgat cctcgacgtt gatggcgtac tgtcagatgg cctgatttat atgggcaata
    63181 acggcgaaga gctgaaagcg ttcaatgttc gtgacggtta tggcattcgt tgtgcgctca
    63241 cctctgatat tgaagtcgct atcattaccg ggcgaaaggc taaactggta gaagatcgtt
    63301 gtgccacatt ggggatcact cacttgtatc aggggcagtc aaacaaactg atcgccttta
    63361 gcgatctgct ggaaaaactg gcgattgccc cggaaaatgt ggcttatgtc ggcgatgatc
    63421 tcatcgactg gccggtaatg gaaaaagtgg gtttaagcgt cgccgtggcc gatgcgcatc
    63481 cactgttgat cccgcgcgcc gattacgtaa cgcgcattgc tggcggtcgt ggcgcagtgc
    63541 gagaagtttg cgacttatta ctcctggcgc agggcaaact ggatgaagcc aaagggcaat
    63601 cgatatgagt aaagccagac gttgggttat cattgtgcta tcactggcgg ttctggtgat
    63661 gatcggcatt aatatggccg aaaaagacga taccgcccag gtggtcgtca acaacaatga
    63721 tcccacctat aaaagcgagc atacggacac gctcgtctat aacccagaag gggcactaag
    63781 ctatcgattg attgctcaac acgttgaata ttattccgat caggccgttt cgtggtttac
    63841 gcagccggta cttaccacgt ttgataagga taaaatcccg acatggtccg taaaagcaga
    63901 taaagccaag ctgaccaatg accggatgct ctatttatat ggacacgttg aagtcaatgc
    63961 actcgtgccg gactctcaac ttcgcagaat cacgacggat aacgcgcaga tcaatctggt
    64021 gacgcaggat gttacctctg aagacctcgt cacgttatac ggaacaacat ttaactccag
    64081 cggtctgaaa atgcgcggca acttacgcag caagaacgcc gagctgattg aaaaggttag
    64141 aacatcctat gaaattcaaa acaaacaaac tcagccttaa tcttgtgctt gccagctcac
    64201 ttctggccgc cagcattccg gcatttgccg taaccggaga cactgatcag ccgatccaca
    64261 ttgaatcgga ccagcaatct cttgatatgc aaggcaacgt ggttaccttt accggtaatg
    64321 tcatcgtcac ccagggcacc atcaaaatta atgccgacaa agtggtcgtt acccgtccgg
    64381 gcggcgaaca aggtaaagaa gtgattgacg gctacggtaa accggcaacg ttctaccaga
    64441 tgcaggacaa cggtaaaccc gttgaaggtc acgcttccca gatgcactac gaactggcaa
    64501 aagattttgt cgttctgacg ggtaatgctt atctgcagca ggtcgatagc aacattaagg
    64561 gcgataagat cacttacctg gtgaaagagc agaaaatgca ggctttcagc gacaaaggca
    64621 agcgcgtaac caccgttctg gtgccgtcgc agctgcagga caaaaacaat aaaggccaga
    64681 ccccggcaca gaagaagggt aattaattcg ttatggcaac attaactgca aagaaccttg
    64741 caaaagccta taaaggccgt cgcgtggtag aagacgtcag cctgaccgtc aactccgggg
    64801 aaattgtcgg tctgctgggg ccaaacggtg ccggtaagac caccactttc tacatggttg
    64861 ttggcattgt gccgcgcgat gcgggcaaca tcattattga tgatgacgat atcagtctgc
    64921 tgcctctgca tgcacgcgcg cgccgcggta tcggctatct gccgcaggaa gcctccattt
    64981 tccgtcgcct cagcgtttac gataacctga tggcggtact gcaaattcgt gatgacttgt
    65041 ctgctgaaca acgtgaagac cgcgcgaacg agttgatgga agagtttcac attgagcacc
    65101 tgcgtgacag catggggcag tcactctccg ggggtgaacg tcgccgtgta gaaattgccc
    65161 gcgcactggc tgcgaatccg aaatttattc tgctcgacga accgtttgcc ggggttgacc
    65221 cgatctcggt tatcgacatt aaacgcatca ttgagcacct gcgcgacagc ggcctgggcg
    65281 tactgatcac tgaccacaac gtgcgtgaaa cactggcggt ttgtgaacgc gcttatatcg
    65341 tcagtcaggg gcatttgatc gcccacggca cgcctacaga aatcttacaa gacgaacacg
    65401 ttaagcgtgt ataccttggg gaagacttca gactctgata gggtagaagt ttgcgacgtt
    65461 ttagcaggag agtacgattc tgaacatgaa gcaaggtttg caactcaggc ttagccaaca
    65521 actggcgatg acgccacagc tccaacaggc aattcgtctg ttgcagttgt cgacgctgga
    65581 acttcagcag gagctacagc aggcgctgga gagcaatccg ctgcttgagc aaatcgacac
    65641 tcatgaagaa atcgacaccc gcgaaacgca agacagtgaa acactggaca ccgccgacgc
    65701 gctcgaacaa aaagagatgc cggaagagct gccgctcgat gccagttggg acaccattta
    65761 caccgctggt acaccatccg gcaccagcgg tgactacatt gacgacgagc tgccggtcta
    65821 tcagggcgaa acgacacaga ccttacagga ttacctgatg tggcaggtcg agttgacacc
    65881 gttttccgac actgaccgcg ctattgctac ctctatcgtc gatgccgttg atgacaccgg
    65941 ttatctgact gtcccgctgg aagatattct cgaaagtatg ggcgatgaag agattgacat
    66001 cgacgaagtt gaagccgtcc ttaagcggat ccaacggttt gatccggtcg gtgtggcggc
    66061 aaaagatctg cgtgactgcc tgctgatcca actctcccaa ttcgataaga ccacgccatg
    66121 gctggaagag gccagactga tcattagcga tcatctcgat ctattagcca atcacgactt
    66181 ccgcacttta atgcgcgtca cgcgtctgaa agaagatgtg ctgaaagaag ccgtcaatct
    66241 gatccagtcg ctcgatccgc gccccgggca gtcgatccag actggcgaac ctgagtatgt
    66301 cattccagat gtgctggtgc gtaagcataa cggccactgg acggtagaac tcaacagtga
    66361 cagcattccg cgtttgcaaa tcaaccagca ctacgcctcg atgtgcaata acgcgcgcaa
    66421 cgatggtgat agccagttta tccgcagcaa tctgcaggat gccaaatggt tgatcaagag
    66481 tctggaaagc cgtaacgata cgctactgcg cgtgagtcgc tgtatcgttg aacagcagca
    66541 agccttcttt gagcaaggcg aagaatatat gaaaccgatg gtactggccg atatcgccca
    66601 ggccgtcgag atgcatgaat cgacgatatc tcgcgtgacc acgcaaaaat acctgcatag
    66661 tccacgaggc atttttgaac tgaaatattt cttttccagt cacgtcaata ctgaaggcgg
    66721 gggtgaagcc tcttccacag cgattcgcgc actggtgaag aaattaatcg cggcggaaaa
    66781 cccagcgaaa ccattgagcg acagcaagtt aacctctttg ctgtcggaac aaggtatcat
    66841 ggtggctcgc cgcactgttg cgaagtaccg agagtcttta tccattccgc cgtcaaacca
    66901 gcgtaaacag ctcgtttgac ccaaccgata aggaagacac tatgcagctc aacattaccg
    66961 gaaataacgt cgagatcacc gaggcactgc gcgaatttgt tacagccaaa tttgccaaac
    67021 ttgagcaata ttttgaccga atcaaccagg tctatgttgt tctgaaagtg gagaaagtca
    67081 cccacacctc agatgcaaca ctgcatgtaa acggtggtga aattcatgcc agcgcggaag
    67141 gtcaggacat gtacgctgcc attgatggct taattgataa gctggcacgc cagttgacca
    67201 aacataaaga taaactgaaa caacactaat tgtccgggca attagcatgt gcatggcggc
    67261 ctgttgtgcg gcacaacggg ccatttgtac ggttaatgct ccgagcctgt tccactgttt
    67321 gagtgggcag gttcttaggt gaaattatga caaataatga tacaactcta cagcttagca
    67381 gtgttcttaa cagggaatgt acgcgaagcc gcgtccactg tcagagcaaa aaacgcgccc
    67441 tggaaatcat cagcgagctg gcagcgaaac aacttagcct ggcgccccag gtggtttttg
    67501 aagctatcct gacgcgggaa aaaatgggca gtaccggtat cggcaatggt attgccattc
    67561 cgcatggcaa actggaagaa gatactctgc gcgccgttgg cgttttcgtt cagctcgaaa
    67621 cgcctatagc tttcgatgcc atcgacaacc aaccggtgga cttacttttt gccctgctgg
    67681 tgccggcaga ccaaactaaa acgcacctgc atactctgtc gctggtagcg aaacgtctgg
    67741 cggacaaaac catctgccgc cgtttacgcg cagcccagag cgatgaagag ctgtatcaaa
    67801 tcattacgga taccgaaggt actccggatg aagcgtagtt attcggtaat gtctctttta
    67861 gacgttgtga ggagaaacag tacatggtac tgatgatcgt cagcggacgt tcaggttcag
    67921 gtaaatctgt cgccctgcgt gcgctggaag atatgggttt ttactgcgtg gataaccttc
    67981 ccgtagtgtt gttacccgat ctggctcgaa ctctggccga tcgagagatt tctgccgccg
    68041 tcagcattga tgttcgtaat atgccggagt caccagaaat attcgaacag gcgatgagta
    68101 acctgcctga cgctttctca ccgcaactac tgttcctgga tgccgaccgt aataccttaa
    68161 ttcgtcgtta cagtgacacg cgccgactgc atccgctttc cagcaaaaac ctgtcgctgg
    68221 aaagtgctat cgacaaagaa agcgatttgc tggagcctct gcgttcgcga gcggatctga
    68281 ttgttgatac ctcagaaatg tccgttcacg agctggcaga aatgctgcgt acccgtctgc
    68341 tgggtaaacg tgaacgtgaa ctgaccatgg tctttgagtc tttcggcttc aaacacggta
    68401 tccctatcga tgcagattac gtctttgacg tgcgcttctt gccgaacccg cactgggatc
    68461 cgaaactgcg tccaatgaca ggtcttgata aacctgtcgc cgcgttcctc gaccgccaca
    68521 cagaagtaca caattttatc taccagacgc gaagctatct tgagctatgg ttacctatgc
    68581 tggaaaccaa caaccgtagc tacctgacgg tcgccattgg ttgtaccggc gggaagcacc
    68641 gttcggtgta tattgcagag caactggcag actacttccg ctcgcgcggt aaaaacgtcc
    68701 agtcacgcca tcggacgctg gaaaaacgta aaccatgacc gtcaagcaaa ctgttgaaat
    68761 tacaaacaag ctgggcatgc atgcccggcc tgcaatgaag ctgtttgaat taatgcaggg
    68821 ttttgacgct gaagtgctgt tacgtaatga cgaaggcacc gaggctgaag ccaacagcgt
    68881 tattgcgctg ctgatgttgg attctgccaa aggacggcag attgaagttg aagcgaccgg
    68941 tccacaggaa gaggaagcac tggccgccgt tatcgccctc tttaactctg gttttgatga
    69001 agattaatct tcatcacttt gacattcaaa tcccttcaag ctccccccgg caataagaaa
    69061 agcgcgtact gaaatcgtta tcaatcaacg tcgattgtac atttcgtgcg ctttttactt
    69121 tcagaagaac cttaagaaaa ccttaagaag cattgtttag atttcgttta agttaatcga
    69181 ccatactgga gatcgtcaga aaatatttcc aggagatggc atgattcgct tatctgaaca
    69241 aagtcccctg ggcaccggaa gacatcggaa atgttatgcg catccggaag atgcccaacg
    69301 ctgtatcaag attgtctacc atcgtggcga tggtggcgat aaagagatcc gccgtgagtt
    69361 aaagtactac gcgcatcttg gtcgccggtt aaaagactgg agtggaatac cgcgctatca
    69421 cggtaccgtt gagacggatt gcggaaccgg ttatgtctac gatgtgattg ccgatttcga
    69481 cggcaagcct tccattacgc tgaccgaatt tgcagaacaa tgccgttacg aagaagacat
    69541 tgcacaactg cgccagttac tgaaacagct gaagcgttat ttgcaggata accgtatcgt
    69601 gacgatgtcg ctgaagccgc agaatatcct ctgtcaccgc atcagtgagt cagaggttac
    69661 ccccgtcgtc tgcgacaaca ttggtgaaag cacattgatt ccactggcga cctggtcgaa
    69721 gtggtgttgt ctgcgtaagc aagagcggtt atggaaacga tttatcgccc aaccggcgct
    69781 ggctattgcc ctgcagaaag atctgcaacc tcgtgagagc aaaacgctgg ccctcacttc
    69841 gcgcgaagct taatcaagct ggtgttgttg cataaacggc tcaccgccta actgatacat
    69901 ctgccgtaaa atccacacct gacggctacg cacgtagccc gatggcgcgg agactttgaa
    69961 acgaagtgga ttaggtaata cagctgccag caatgcagct tccgaccggg taagtttgct
    70021 cgcgggtttg tggaaataac gttgtgccgc agcttcgacg ccaaacacac cgtcgccaaa
    70081 ttcggcgata ttcaggtaaa ccgtcaggat acgctttttg ctccagactg tttctatccc
    70141 cagcgttaat ccggcttcca gcccttttcg aacccagcta cgcccatccc ataaaaagag
    70201 atttttggct gtctgctgag aaatcgttga agcaccgcga atacggtttt cattgcgctc
    70261 gttgtgcgcc agggcttgct caatggaagc gacatcaaag ccccagtgct caggaaattt
    70321 ctgatcttct gcggcaatca ccgccagtcc catccacggc gagatttgat ccatactgac
    70381 ccagtcagaa tgtgccacgt aacgaaaatt gccatgcagc caggcgctga cttgccgctc
    70441 gaccattact gctgagaaag gaacaggcgc aacgctaaac aacgcgatgc ccccgcccca
    70501 gaaaatggcg aggacaacca ttaaccgcag aagaaaacgg cgaacgaaac taaacaccgt
    70561 taagcggctt ttactcattc agccagaacc agcacgcggg aaaccagctt atcaatgccg
    70621 ctcgccgctt ctgcaatgtt ctgcgccagc atatatgccg gggtggtgac aatcttattg
    70681 tcttcatcaa ccacgatatc atcgacagga cacggcacat gctccgcgcc catctcttcc
    70741 agcacttctg cggtatcgat atcagtaccg atggtcaaac gtagcgggaa atcgaaaatt
    70801 ttcggcagta tcgccggtgc aatacacata aaaccaagcg gttttccggc ctgatgcatc
    70861 gcttgtgcca gcgcctttaa ttcacggtca acggtgcatt cgctaccaag actggcaaaa
    70921 ttgcttaaat tcttcgccgc gccaaaaccc cccggcacaa tcaacgcatc cagttcagcg
    70981 gcatcggcct gggccagagg acgaatttca ccgcgcgtta ttcgtgccgc ttcaatcagc
    71041 acattgcgcg tttccgtcat cgcttcacca gttaaatggt tgataacatc aacctgctgc
    71101 ttatccggtg caaagcagac cgcctgcgca ccgctgcgtg aaatagctaa caacgtcaac
    71161 accgcttcat gaatttcaga accgtcatag acgccgcatc cgctcagaat tacgccaatt
    71221 ttcttcattg tgatcttcct ttcgcaattg actgaaacac attaattttt ttaataaaaa
    71281 tgctacgcat cacacattta actgattcat gtaacaaatc atttaagttt tgctatctta
    71341 actgcgtgcg gcctgaaaaa cagtgctgtg cccttgtaac tcatcataat aatttacggc
    71401 gcagccaaga tttccctggt gttggcgcag tattcgcgca ccccggtcta gccggggtca
    71461 ttttttagtg gcttttgcca cccacgcttt cagtacttct acgtcgtgac gccactcttc
    71521 tttcatctct tcaatccatt caccgacgtt atcttcccag gcgggaaggt caggagactg
    71581 aatttgctga cccagctgtt gcagatggcg taaccccact gaccccgccg cacctttaat
    71641 tttatgccct tcctcaacaa tgcctttttt atcctgcgcc gtcagattcg actccagcac
    71701 gctgacatag cccggcatca tcctctcaaa caccgctaac ccgtcggtga tcagcttcgg
    71761 tcctacaagt tcgagatact gttccagcat gggaatatcg agcaatgctt ctgatttact
    71821 gttctcttct gtcgtcaccg tactctcctc atcatcctgg gtatcccaga atttcttgat
    71881 catcgcggtt agcgccggaa cagaaagcgg cttactcagc acatcatcca ttccagcatt
    71941 gaggtactct tgtttgtctt tcagcacgtt agcggttaag gccaccagcg gcggtaaatc
    72001 ctcgcgcgga taacgcttcg tcagttcacg agagatatcc agcccggtca tatctggcaa
    72061 ctgaatatcc agcaacacca ggtcgtattc gcccggttta aacatctcca gcgccgcctt
    72121 gccggtcatg gcgacatcaa cgctgttacc taatttttcc agcacagagc gcgcgacaat
    72181 cacgttcagt tcaatgtctt ccaccagcag cacattcagc gccggtaaag gcatatcgtc
    72241 ttcatcaaac gcatcatcaa cctcttctgc taccgacggt gcgtggatcg tcaacgtaaa
    72301 ggttgaacct ttgccctgtt cgctggtaac cgtaatatcg ccgcccatat ttttcgccag
    72361 acgacgagaa acggccagac caataccggt gccggtggca ggtttaccgc catgactgtc
    72421 tttcacctgg taatacatgg cgaagatttt atccagctca tcctccggaa tgccgatacc
    72481 agagtcttcc acttcaaaat gcagcatatc gccttcatcg tagcgcacgc gcacggtaac
    72541 ctggccttgc tgagtgaatt tgacggcgtt actgatgagg ttccacaaga tctgccgtaa
    72601 acgcgtcccg tcggtaatga cctgatgcgg taatggcagc gtcggctcca gattaaagcg
    72661 caatcctttt tgttgcgcct gcaatgcgga gagattttcc agatcggcaa ggaagctggt
    72721 gaaatcaacc ggttgattat caagctggac cttgcgccgt tccatcttat ccatgtcgat
    72781 aatatcgttg aagatattcc ccagcgtgac agccgaaaca tggatggttt tgagatattt
    72841 ttcctgctcg gcggtgagtt cagtatccag cagaatgcgg ctcaggccga cgataccgtt
    72901 cagcggtgta cgcaattcgt gactgatggt ggagataaac gtcgttttgt cgcggctggc
    72961 gcgttcaagc gcatcctgat accgcttacg ctcggtaatg tcgcgaccaa agcccatcaa
    73021 accgtgacgt ttacccacgc ggtcgtagta cggtacttta cggatttcaa agcaggcttt
    73081 gcgtccgtcg ggataatcca gccactgctc ataggtcagt gacacattat gacggaacac
    73141 tttttcatcg gtttcaatga ctttcgcagc ggcttccggt gagtaaacat cagcaggttt
    73201 cagatgaacc agttgttttt cgctttttcc ggtcagcagc tccatcgcgc ggttacagcc
    73261 ggaaaactct ttatcttcgt tacgataaaa aaccaggtcg ggtgaagcgt caaggaagga
    73321 acgtaagaat gaggattgct gctcgagctg aatttgtgtc tcttcgcgct ctttgatttc
    73381 aattttcagt tggccgaagg tttcctgcag ttctgcttcc gctttttcac gaacggcgat
    73441 ttcctgattt agctgggcaa tattatcttt taactgaacg ttgaggctca aatcgcgctc
    73501 gcgcatctcc tccagttttt gcaccagtcg tgacagacgt tgtcgtgact cctccagttg
    73561 ctcgacgacc accgatagaa agtagaccgc ccacggcgta atcagcaaac caaagaagat
    73621 agaacgaata acatcaatgc tttcgacctg gccatgcagc accatggtta ccgccatttg
    73681 taccacaatg gcaagaacga cgagggccag cgccagcaac attgagaagc gcaccagacc
    73741 taacttcatc atcaggtcaa cataatactg cgccagcaga cgaatttgct tcattaggga
    73801 attccttcac gacaacctgc cccaataata cccaattctg acagttacgt tataaattgt
    73861 gtgagaaatg cggaattccc ctcaatggcc tacgccgttg gaggtagcca cggacgtccc
    73921 agcgccgatc cctgtacgcc atgttcattc agataacgat ccagcccgac cattcccgtc
    73981 catcgatttt cgcaccacag cggagcaagc agcgtcgggc gacgggcgct ggcggaaatg
    74041 cggtggtaga tcacttccgg tggcgtatgg cgaatcattt ctccggcagt gagcgtgtaa
    74101 tcctccagtt caattccgtt caaacgcccc gcttcccagg ctttcgccat aatgctgcct
    74161 ttcactatat gcagcggatg cagctttatg ccgtccacgc cggtttcaac cactcgctcc
    74221 agcgtttgca aacattcagc ctgcccttcg ccaggcaggc caacaattaa gtgagaacac
    74281 accttcagcc cacgctcacg cgccagctgc gtcgtgcgct gatagcaggc aaaatcatga
    74341 ccgcgattga tgcgatgcag cgttttgtcg tgggcggttt gtagccccag ctccagccac
    74401 acttcgtaac cctggtcctt atattcgcaa agcagatcca gcaccgcatc cggcacgcag
    74461 tccgggcggg taccaacaca caaaccgaca atattggcct ggctcaccgc ctgctgatac
    74521 atagaacgca gcacctgaac ttccgcaaag gtgctggtat acgcctgaaa gtaggccaga
    74581 tagcgtttag cgcggttaac taaattcgcc tggtgcgcca gttgctcggc aatggaacga
    74641 tgctgctgcg cttcatcggc aaacgaggcc acattacaga atgtgcagcc accacgcccg
    74701 atggtaccgt cacggttagg gcagctaaaa ccgccgtgca gcgtcagctt atgcaccttt
    74761 tgcccataac gacgggtgag atcaccacca aacatattga ctaatttctg taactgcata
    74821 atctgataga ccgcgccttg aaaagaggcc aaagcctgcc atttttagct caattcggcg
    74881 atgacctgga tcaatcgtcc cggcctgctt ttatcaactg cataatcaat caaaattacc
    74941 gaagtttcat gcataatcac ataaatcact tttgcttatc ttgtgtcaga tttttttatc
    75001 tccttatgga ttttaggcaa aaacagtagc atgaaacgtc attaccaatt aaggcagtat
    75061 aaaatgctgg ttttctcgtc agttcaaggc aggataaggg ttaacacgcc tttatgacag
    75121 tcaggaattg gctgtttctc taacgacgcc ccttttagcc ttaattagaa aatccatttt
    75181 aatttcagtc aattaataaa gaattttgcg ctaaagcaca tttctgtacc aataagcttg
    75241 ccatttgacc tgtatcagct ttcccgataa gttggaaatc cgctggaagc tttctggatg
    75301 agcagcctgc tcatcatatt tatgcagtaa ttgagatccc ctcttcaccg tattaaccga
    75361 tgcgaaaagg acaacaaggg ggcgaatgcg aggcgcgcgt atgacacgca aaccccgtcg
    75421 ccacgctctt tctgtgcccg tgcgcagcgg ttcggaagtg gggttcccgc agagcctagg
    75481 ggaggttcac gatatgttgt acgataaatc ccttgagagg gataactgtg gtttcggcct
    75541 gatcgcccac atagaaggcg aacctagcca caaggtagtg cgtactgcaa tacacgcact
    75601 ggcccgcatg cagcaccgtg gcgcgattct cgccgatggt aaaaccggtg acggttgcgg
    75661 cttgctgtta caaaaaccgg atcgcttttt tcgcatcgtt gcgcaggagc gcggctggcg
    75721 tttagcgaaa aactacgccg tcgggatgct attcctgaat aaagatcctg aactcgccgc
    75781 tgccgcacgc cgcatcgttg aagaagaact gcaacgcgaa accttgtcga ttgtgggctg
    75841 gcgtgatgtc cccactaacg aaggcgtgtt gggtgaaatc gccctctcct ctctgccacg
    75901 cattgagcaa atttttgtga acgccccggc aggctggcgt ccgcgcgata tggagcgccg
    75961 tctgtttatc gcccgccgcc gcattgaaaa gcgtctcgaa gccgacaaag acttctacgt
    76021 ctgtagcctg tcgaatctgg taaacatcta taaaggtctg tgtatgccgg cggatctgcc
    76081 gcgcttttat ctggatcttg cggacctgcg tctggaatcg gccatttgcc tgttccacca
    76141 gcgcttttcc actaacaccg taccgcgctg gccgctggca cagccgttcc gctacctcgc
    76201 gcataacggt gaaatcaaca ccatcactgg taaccgccaa tgggcgcgtg cgcgtaccta
    76261 taaattccag acgccgctga tccctgacct gcacgacgcc gcgccgttcg tcaacgaaac
    76321 cggctctgac tccagttcca tggataacat gctggaactg ctgctggcag gcgggatgga
    76381 tatcatccgc gccatgcgtc tgttagtacc acccgcctgg cagaacaacc cggatatgga
    76441 cccggaactg cgtgccttct ttgactttaa ctccatgcat atggagccgt gggatggccc
    76501 ggcaggcatc gtgatgtctg acggtcgttt tgccgcctgt aacctcgacc gtaacggtct
    76561 gcgtccggcg cgctacgtca tcaccaaaga taagctcatc acctgcgcct ctgaagtcgg
    76621 tatctgggat tatcagcctg acgaagtggt cgaaaaaggc cgcgtggggc caggcgaact
    76681 gatggttatc gacacccgca gtgggcgtat tctgcactcg gcagaaaccg atgacgatct
    76741 gaaaagccgc catccatata aagagtggat ggagaaaaac gtccgccgac tggtaccgtt
    76801 tgaagatctg cccgatgaag aagtgggtag ccgcgaactg gacgacgaca cgcttgccag
    76861 ctatcagaaa cagtttaact acagcgcgga agagctggat tcggtgatcc gcgtactggg
    76921 tgaaaacggt caggaagcgg tcggttcgat gggcgacgac accccgttcg ccgtgctctc
    76981 cagccagccg cgcattattt acgactactt ccgccagcag tttgctcagg taaccaaccc
    77041 gccaatcgac ccgctgcgtg aagcgcatgt tatgtcgctc gccaccagca ttggccgcga
    77101 aatgaacgtc ttctgcgaag ctgaaggcca ggcgcaccgt ttaagcttta aatcgccgat
    77161 tctgctctat tccgatttca aacagctcac gacgatgaaa gaggatcact accgcgcaga
    77221 tacgctggat atcacctttg acgtcactaa aaccacgctc gaagcgacgg tcaaagagct
    77281 gtgcgacaaa gccgaaaaaa tggtacgtag cggcaccgtg ctgctggtgc tctccgaccg
    77341 gaatatcgcg aaagatcgcc tgccggttcc ggctccgatg gcggttggcg cgatccagac
    77401 ccgtctggtc gatcaaagcc tgcgttgcga tgccaacatc atcgtcgaaa ccgccagcgc
    77461 ccgtgatccg caccacttcg ccgtgttgct gggtttcggc gcgacggcta tttatccgta
    77521 ccttgcctat gaaacgctgg gccgtctggt agacacccac gcgattgcca aagattatcg
    77581 taccgtgatg ctcaactatc gtaacggcat caacaaaggc ttgtacaaaa tcatgtccaa
    77641 aatgggcatc tccaccatcg cctcttaccg ctgctcgaaa ctgtttgaag cggtcggtct
    77701 gcacgatgat gtcgtgggcc tgtgcttcca gggggcggtt agccgcattg ggggggctag
    77761 ctttgaagac ttccagcagg atctgctgaa tctgtcgaaa cgtgcctggc tggcgcgtaa
    77821 gcccatcagc cagggcggcc tgctgaaata cgtgcacggc ggcgaatacc acgcctacaa
    77881 cccggacgtg gtgcgcacgc tgcaacaggc ggtacagagc ggcgagtata gcgactatca
    77941 ggaatacgcg aagctggtta atgagcgtcc ggcaaccacg ctgcgcgatc tgctggcaat
    78001 tacgccgggt gaaaacgcgg tcaacattgc tgatgttgaa ccggcaagcg aactgtttaa
    78061 acgcttcgat accgccgcga tgtctatcgg cgcgttaagc ccggaagccc acgaggcgct
    78121 ggcggaagcg atgaacagca tcggcggtaa ctcaaactcc ggcgaaggcg gtgaagaccc
    78181 ggcgcgctat ggcactaaca aagtgtcgcg catcaagcag gtggcgtccg gtcgctttgg
    78241 ggttactccg gcgtatctgg tcaatgccga cgtcattcag ataaaagtcg cccagggcgc
    78301 gaagccgggt gaaggtggtc agttaccggg tgataaagtc actccttaca ttgccaaact
    78361 gcgctattcg gtgccgggcg tgacgctgat ctccccgccg ccgcaccacg atatctactc
    78421 tattgaggat ttagcgcagc tgattttcga cctcaagcag gtcaatccaa aagcgatgat
    78481 ctccgtgaag ctggtttccg aaccgggcgt aggcaccatc gcgactggcg tggcaaaagc
    78541 ttatgccgat ttaatcacca tcgcaggcta tgacggcggc accggcgcaa gtccgctttc
    78601 atcggtgaaa tacgcaggct gtccgtggga gctggggctt gttgaaaccc aacaggcgct
    78661 ggttgccaac ggcttgcgtc acaagatccg tttgcaggtc gatggcggcc tgaaaacggg
    78721 tgtcgatatc atcaaagcag cgattcttgg cgcggaaagt tttggcttcg gcactggccc
    78781 gatggtagcg cttggctgta aatatctgcg aatttgccac ctgaacaact gcgccacggg
    78841 tgtagcaact caggatgaca aactgcgtaa gaatcactat cacggcctgc cgttcaaagt
    78901 gactaattac tttgagttta tcgcccgtga aacccgcgag ctgatggcac agctgggggt
    78961 aacgcgtctg gtggatctga ttggtcgcac cgacctgctg aaagagctgg acggtttcac
    79021 cgccaaacag cagaaactgg cactgtcgaa gctgctggag actgccgagc cgcatcctgg
    79081 taaagcgctc tactgcaccg aaaacaaccc gccgtttgat aacggcctgc tgaacgcgca
    79141 gttgctgcaa caggcaaaac cgtttgtcga tgaacgccag agcaaaacct tctggttcga
    79201 tatccgcaac accgaccgtt ctgtcggcgc gtcgctttca ggctatatcg cccagacgca
    79261 cggcgatcag gggctggcag ccgatcctat caaagcgtac ttcaacggca ccgcaggcca
    79321 gagcttcggc gtgtggaacg cgggcggcgt ggaactgtac ctgaccggtg atgccaacga
    79381 ctatgtcggt aaaggcatgg cgggcggctt aatcgccatt cgtcctccgg ttggttccgc
    79441 cttccgcagc catgaagcga gcattatcgg caacacctgc ctgtatggcg cgaccggtgg
    79501 tcgtctgtat gccgcaggcc gcgcgggtga acgtttcggc gtgcgtaact ccggtgctat
    79561 caccgtggta gaaggcattg gcgacaacgg ctgtgaatat atgacgggtg gtatcgtctg
    79621 tattctgggt aaaaccggcg ttaacttcgg cgcgggcatg actggcggct tcgcttacgt
    79681 tctcgatgaa agcggcgatt tccgcaaacg cgttaatccg gagctggtcg aggtcttaag
    79741 cgttgacgat ctggcgatcc atgaagagca tctgcgtggt cttatcaccg agcatgttca
    79801 gcataccggt tcccagcgtg gtgaagagat tctggcgaac tggtcaacct tcgccactaa
    79861 atttgcgctg gttaaaccga agtccagtga tgtaaaagca ctgctgggcc accgtagtcg
    79921 tagcgcagca gagttgcgcg tgcaggcgca gtaaggggta gcaacaatga gtcagaatgt
    79981 ttatcaattt atcgacctgc agcgcgttga tccgccaaag aaaccgctga agatccgcaa
    80041 aattgagttt gttgaaattt acgagccgtt ttccgaaggc caggccaaag cgcaggccga
    80101 ccgctgcctg tcgtgcggca acccatactg cgagtggaaa tgcccggtac acaactacat
    80161 cccaaactgg ctgaagctcg ccaatgaggg gcgtattttt gaagcggcgg aactgtcgca
    80221 ccagacaaac accctgccgg aagtttgcgg acgtgtctgc ccgcaagacc gtctgtgcga
    80281 aggttcctgc accctgaacg atgagttcgg tgcggtgacc atcggcaaca ttgagcgtta
    80341 tatcaacgat aaagcgttcg agatgggctg gcgtccggat atgtccggcg tgaaacagac
    80401 cggtaaaaaa gtggcgatta tcggcgcagg tccggcgggt ctggcgtgcg cggatgtcct
    80461 gacacgcaac ggcgtaaaag cgatcgtctt cgaccgtcac ccggaaattg gcggcttgct
    80521 gaccttcggt attccggctt tcaagctgga aaaagaggta atgacacgcc gccgtgaaat
    80581 cttcaccggc atgggtattg aattcaaact caataccgaa gtgggccgcg acgtgcagct
    80641 ggacgatctg ctgagtgatt acgatgccgt gttccttggc gtcgggactt atcagtcaat
    80701 gcgcggcggg ctggaaaacg aagacgccga tggcgtgtac gcagcactgc cgttccttat
    80761 cgccaacacc aaacagttaa tgggctttgg tgaaacccgc gacgaaccgt tcgtcagcat
    80821 ggaaggcaaa cgcgtggtgg tccttggcgg tggcgacact gcgatggact gcgtgcgcac
    80881 gtctgtacgc cagggggcaa agcacgttac ctgtgcctat cgtcgtgatg aagagaacat
    80941 gccgggttcc cgccgcgaag tgaaaaacgc gcgggaagaa ggcgtagagt tcaaattcaa
    81001 cgtccagccg ctgggtatag aagtgaatgg caacggcaaa gtcagcggcg taaaaatggt
    81061 gcgcacagaa atgggcgaac cggatgccaa aggccgtcgc cgcgcggaga ttgtggcagg
    81121 ttccgaacat atcgttccgg cagatgcggt gatcatggcg tttggtttcc gtccacacaa
    81181 catggaatgg ctggcaaaac acagcgtcga gctggattcg caggggcgca ttatcgcccc
    81241 ggaagacaac gacaacgctt tccagaccag caacccgaaa atctttgctg gcggcgatat
    81301 cgtccgtggt tccgatctgg tcgttaccgc cattgccgaa ggtcgcaaag cggcggacgg
    81361 cattatgaac tggctggaag tttaagcgag gtaacaataa attcgccaga cagtgtctgg
    81421 cgaatttata aaacgagcga tccaactaaa ttttaagcca ttaaatcagc cttaaccttc
    81481 actaccacct ttttaatctc accaggttcg ccaacaacgc atcccggttt atgcggttct
    81541 cctggcataa acacggcgaa cattcccggt ttcaggatga tggcttgctc gttttcaatg
    81601 gcgctgcaaa gctgataatc atcttcatgg tggaactctt cacactgacg cgcagtgcct
    81661 gccatgccaa acagaatccg ttcctcaccg tttaataaca gctggatatc aatgtattgt
    81721 tcgtgcaatt cggctttttt ctcaacgggc gattgagtat taaatgtcat gacattcata
    81781 aagatattgt cgccctgtaa ttcgtaacgg ccagtcgctt tttcttgcgg tctggcagct
    81841 aatgccaacg ttaacgcgtc ctgtaacgca ggatgtaatc cggcagacgg taatgactgt
    81901 acttcaccca tcatcataat atttctccct gggccaacag cgcagcccca agtaaacctg
    81961 catcgtggcg gtagtgcgcc gccagtaaat caacatgaaa tgccgctggc tcctgcgcca
    82021 gatacgtttc caccagcgcc agataccctt ctgccagacc aacgctgcca ccgaccacca
    82081 cgcactggca atcagttgtg gctttaatat cagcgatcag ccttgcaagc gtacgtgcgg
    82141 agcggtgaat cagttgctgc gcctgctcat caccctgccc ggcgcgcgtg aaaatagttt
    82201 tcgcattcgc accagccaac tccccctgcg ctgccgctgc aatgccgcga ccagaagcaa
    82261 tcgcttccac gcaacctgtg cgtccacagc cgcagactgg accatgcgga tcggcaagcg
    82321 tatgcccgat atgccccgcc agaccgccag ggccggtacg cagtttaccg ccgctcacta
    82381 caccgccgcc aacgccggtg gaaacagtga taaacaccat atcggttata tcgccatcca
    82441 gcgcctgata ctccgcccat gctgcggcct gcgcatcatt aatggcaatg gtcggcaaat
    82501 tggtaagttg ctccagcgtt ttgactaacg gaaagtgtaa caatccaccg agattatgcg
    82561 gattcagcgc cagcaagctg ccgtcgcgaa taattccggt tgaagcgatg gcaacccgct
    82621 gcgcatgtgc ttgcaacgga gagactaatg cggataaggc atcacgcagc gcctgcggag
    82681 tctggctggc aggcgtagga agttctcggc gatcgcggat ctgcccgtca gcgccaatca
    82741 gcgcggcggc aagtttagta ccgccgatat caatcgccag tgtggtcata gcaccgcctt
    82801 tttcatcgct gtgttgtacc actgacaaat gtgttcaaga cgcgtaatgg cagaaccgac
    82861 cgtcaccgcc cacgcgccgt ggcgcatcgc atccgccgcc tgagcaggcg tgttgtaacg
    82921 cccttcggca atcacccggc atccggcgtc actcaacgtt ttcaccagcg ccagatccgg
    82981 ctcttctggc gtttcaggcg tggtatagcc agaaagcgta gtgccaataa tttcggctcc
    83041 cagcttttgg catgccaggc cgtcttccgg cgttgagcag tcggtcatcg ccagtaaacc
    83101 gtgatggtga atacgtgcca gcagcgtttc aactggcacc ggacgcggtc ggtcggtgcc
    83161 gtcaatcgcg ataatgtccg cgcccgcctg cgccagcgca tcaacatctt caatataggc
    83221 cgtgatgcgt accggagaat cctccagatc gcgcttcaca atcccgataa tcggcacgct
    83281 caccaccgca cgcgtggctt gcagatttgc cacaccttca atacgaatgg cgaccgcgcc
    83341 cgcctgctct gccgctaatg ccatggcggc gacgatttcg ggtttatcga gcgggctgtc
    83401 cggaaccggc tggcaggaga caatcaggcc accgttagct gcgatttttt gatccagttg
    83461 tgcaagtaac gacatacatc ttcccttagc gaaaggcccg gtacagagac cgggcaacag
    83521 gattaacttt tggttttgac taaatcgttt ttggcgctgc caaacggcac ggcaccgcta
    83581 aatggtttgc cgtcgatagc gtcatgagta cgcaatgctt ccgggcgcag ccaacgctga
    83641 acacgagaag gcatatccag accaatcagc aggatcacca caaacgttaa actaaacgag
    83701 agcgagccaa gtgccgttcc cagatccaga cgttgagcga tcaacgcgcc gaggattgga
    83761 gctagtgcac cgcccagtgc gccaacgttg taggtaaagc ccagccccgc cgcacgctgg
    83821 tcggtatcga aataaccgcc gatcagtttc ggtaaaatcc ctgagatgcc ctgaccaagc
    83881 atttgctgga agaacagtaa cagaccgagc acccagacgt ttgcgccgcc aatcgcaaat
    83941 accggaataa tcagcagctg cgaggccagc agactacaaa cgtacgcttt gcgggttccc
    84001 agccagtcac cgaggaagcc accgacgcag caccccaccg ccgcgccaaa gccactaaag
    84061 aacagcacat tggcgacagt atgcgggtca taagccagat cggttttcag atacgttggc
    84121 agcagcgcct gaatcggcca tgagtagagg aaagcaaaca acacgaccac catcagcatt
    84181 acgcccgttg gccagcgttt gcccgtactt tgcaccataa agctgataaa gattgcggcg
    84241 cataacagcc cgagtacagc gacgatcgcg gcattttgca ggttaccggc gaagcagaac
    84301 cacagcgcag tagccgccgc cagtgtcatt acgatattgg caatgcgatg ttcaccacgg
    84361 tagagaatgt ctaccatcgt gcgtactggt gctttacccc cgtgtttctc tttccagtct
    84421 tccgcttccg ggatgttttt acgcagccag agagcaaaga tgattggcaa aatgccgata
    84481 aagaacagcg ctctccagcc ccagaccgga accaccagac tatagacctg agcggcaacg
    84541 acggccccca ctgagaagcc tgaaatcaga aaaccactgg ctttgttacg cagatgcttt
    84601 ggccagcttt caatgacata ggtggcgctg gaaccgtatt cacccgccat ccccatgccg
    84661 atgaccagac gagcgataaa catggtgatg tagcctggtg caaagccgca ggccagcgtc
    84721 ccggccgaga agagaacgat gctggtgacc attgccagac gacgcccgta gcggtcaccc
    84781 atagcgccga gcatcaggcc gccgaaccag cgagagataa aggctgcgga gatcagactc
    84841 gccgcctgca ccgtcgtcag cccgaattca ccctgtactt cggtgagtac cagggcgatt
    84901 aaaacgaaat caaaaccgtc aagcagatat cccaaccagg cagcggaaaa tgcccgccat
    84961 tgtgcgcggt taagatggcg ataccacggg atattctggg ttgtagtact cattgtgagt
    85021 ctcccgcggt gggcgatgcc cacacgcttt ggtatgaaaa ttgtagggta cagatgcgtt
    85081 tatttcccct cacccggtag gggcgagcga ggggaaacaa ctcacccgcg ctcttgcatc
    85141 aactgctggg ccagcgcctt cagttctggc agatattttt catctaccgg accaaacggt
    85201 ttgcggcaca gcggcacaga aacgacatcc atataatgca ggacagtttt caggccgcgg
    85261 aatacgccag ttttgatcag taaatcaatg actttgttgc actcagtttg cagtttctgc
    85321 gcggtctgga tatcgccttc tttcagcgcc ttcacgatcc cctgataacg ccagcccatg
    85381 atgttgtaag tgctgccgat accgccgtct gcgcccgcca gaagaccaga ggcgaagatt
    85441 tcgtcgtaac cgttatagag caccagatca ggatgttcac gacggatctg ctccatctga
    85501 tagagatcgc cagaggtctg tttcagcgca cctacaccag gcagcgtaac cagggtgttg
    85561 atctgatcca gggtcagttt taccccgctc agggctggaa tgttgtacac caccatcggc
    85621 aacccatccg ccgaatcaat aattgcccga tagtgatcgc agtgttcttc aaagctgaaa
    85681 ggatagtaga acggcgtgac ggcggagacg gcatcgaagc cataacgttt agccgatgcc
    85741 gcaagttgtt ggctttcggc ggtgctgacg caaccgacgt gggcgatgag tttaatctta
    85801 cctttcgcct cttcagcgac gatttccagt acctgttcac gctctgaaag gctctgtaca
    85861 aaggcttcgc ctgtcgaacc acccacgtac aaaccgtcga tgccctgctg aatattgaac
    85921 tgaaccaggc ggcgcagact cgctttatcc agtgcttgtt gttggtcaaa aggagtcagg
    85981 agtgcagcca ttacgccacg taaattcgtt gccataaata cctctgaagt gatgcttgtc
    86041 tgataaacga tacaccttta tacctgttat accagatcaa ttaagcaaca ccccatacag
    86101 aaagcttata atgcgatctg cttcactaaa gtggcattat ttctttttgt tggtggtctg
    86161 accgaaggcg tgccaggtag cagagacgct gttgagatgc gattgcaacg cgcgatcggc
    86221 ttcgtcagga tcatgacggc ggatcgcgtc aacgatcgca atatgctgtt gatagctaac
    86281 gttgttatgc tcatgcagag cctgatcggt cacggttgga cgtgcggcga ttagccagtc
    86341 gagcagggca acgtggatcg ccatgaagat tgggttacca gggatctccg ccagaacgcg
    86401 gtggaaatca acgtctgagc gaatgaatgc cgcgttgtta tccagcgact gactgttgat
    86461 ttccagtgct tttgccagca aatcgatttg ctcatcggtg gcgtgttcag ccgcatagcg
    86521 cactaggctg gattcaaaaa acagacgtaa ttgttcgaaa tgggcaatcc caccaggatg
    86581 agaaaggaaa tctttcgcca tgccggaaag ctcgccgatg atggtgtccg cagaaggacg
    86641 tgagacgcgg gcacgttcgc cgttgtttat ttgcaccaga cctttgcgtt ttaacgctgc
    86701 cagcgcttcg cgcaccgaag gacgcccgac gttaaagaac gccatcagtt cgcgttcaga
    86761 cggtaattgt tcaccttcgc caaattcacg acggcggatc atctgttcca gctcttcttc
    86821 caccatttcg gagagttttt tacgcgccag cgggcggctg cgcaagttgc gcccgatagt
    86881 ggtagtagaa tcttcagctt gcggatcaaa tgcgttcata ggactcattc aggaaaatgc
    86941 gttagcggat aataacgacg ctatcgtagc acagtatcaa agtcaggcga aaagatgaca
    87001 gatagcaaaa caggccacat gggcctgttt tcattttact tcacaacgcg taatgccggt
    87061 cgaccaccgc gtggtggctg cggaggttca tcgtcaggat gagtttcatc atcgtgatct
    87121 ggcttgtcgc catcgataac cgacataacg gtttcgttgt ctgccgatgc ctcttcatca
    87181 ttcatgatgc tggcatcttc atcgtaggca gcttcaggct caaacatcgt gcctgcgcca
    87241 ttttcacggg cgtagatagc cagcacggca gccagtggca cagaaacctg acgcggaatg
    87301 ccaccaaagc gcgcgttaaa gcgcacctca tcattcgcca gttccagatt gccgacagca
    87361 cgcggcgcaa tgttgagtac gatttgcccg tcacgcgcat attccatagg aacctgcacg
    87421 cctgggagcg tcacatccac caccaggtgc ggcgtgagct ggttatccag caaccactca
    87481 tagaatgcac gcagcagata gggacgacgt ggtgttagct gtgacaaatc catacagatt
    87541 aactccggcc cagacgcatt tcacgttctg cttcagttaa agaagcaagg aaagagtcac
    87601 gctcaaagac gcgggtcata tagcctttca gctctttcgc acccgggccg ctgaactcga
    87661 tgcccagttg cggcagacgc cacagcagag gagcaaggta gcaatcgacc aggctgaact
    87721 catcgctcag gaagtacggc ttctgaccga agaccggcgc aatcgccagc agttcttcgc
    87781 gcagttgctt acgtgcggca tcggcttcag aagctgaacc gttgatgatg gtgttcatca
    87841 gcgtgtacca gtctttttcg atgcgatgca tgtacagacg gctttcaccg cgagctaccg
    87901 ggtaaacagg catcagtggc ggatgcggga aacgctcatc cagatattcc ataatgatgc
    87961 gagattccca cagggtcagc tcacgatcca ccagggtcgg aacgctctga ttcgggttga
    88021 ggtcaatcag atcctgaggc ggattgtcct tttccacgtg ttcgatctcg aaacttacac
    88081 ctttctcagc cagcacaatg cggacctgat ggctatagat gtcagtagga ccggaaaaca
    88141 gcgtcattac cgaacgtttg ttggcagcga cagccatgaa aacctccagg tatagtcaga
    88201 atttttactg ctaccagcca ccaggtggcc agtcagaagt tgtgttaccc agtaaggaac
    88261 gactctcttt gttcgaaaat caaacaaaaa atgagcaata cccgacattt gggcagaaaa
    88321 ttggatgata gtttaccaga ttttgcgacc tttgtggtga gtcgatgctg gaaatgggga
    88381 aaaagagatg cgctttagtc tgaaatagtt gacttagtcc cttattggcg atgtggtttt
    88441 tgttttacct gtctgtcagg tggcagcaaa aagcaacttt ccatttttta cgctgatgca
    88501 gattttagcc ataaaaaaac ccgccgaagc gggttttttc gcaaattgtt ttctgccgga
    88561 gcagaagcca attaacgttt ggagaactgc ggacgacgac gtgctttacg cagaccgact
    88621 ttcttacgtt caacctgacg agcgtcacga gtaacgaagc cagctttacg cagttcagaa
    88681 cgcagggact cgtcgtattc catcagagcg cgggtgatac cgtgacggat cgcaccagcc
    88741 tgaccagaga taccaccacc tttaacggtg atgtacaggt ccagtttctc aaccatgtcg
    88801 accagttcca gcggctgacg aactaccatg cgagcagttt cacgaccgaa gtactgttcc
    88861 agagaacgtt ggttgattac gattttaccg ttgcccggtt tgatgaaaac gcgagctgcg
    88921 gaacttttgc ggcgaccagt gccgtagtat tgattttcag ccattgccta taatcccgat
    88981 tagatgtcaa gaacttgcgg ttgctgtgcc gcgtggttgt gctcgttacc cgcgtaaact
    89041 ttcagtttac ggaacatagc acgacccagc gggccttttg gcaacatgcc tttaaccgcg
    89101 atttcaatca cacgctcagg acggcgagca atcatctctt caaaggtcgc ttgtttgata
    89161 ccaccgatgt ggccggtgtg gtgatagtac actttgtcag tacgcttgtt gccggttaca
    89221 gcaactttgt cagcgttcag aacgatgatg taatcaccgg tatctacgtg cggagtgtat
    89281 tccgctttgt gcttaccgcg caggcgacga gccagttcag tagccagacg gcccagagtt
    89341 ttaccggtcg cgtcaacaac ataccagtcg cgttttacgg tttctggttt agctgtaaaa
    89401 gttttcatta aaagcttacc caataaatag ttacacgttg gtgaacaccc aaacgtcttc
    89461 aattgttgag gttcacacga caaagtccgg caaacctacc ccttcgaata gcctatgcca
    89521 gcacacaaaa agtttcggga aaaaaaactt tcttgtaacg tggggtcgca ggattataga
    89581 gaagtcgggg tcaaagatcg accccttttt gtgatttgtg gcaggtttta ccccgccaaa
    89641 tgctcgcgct tcaaatactc ttcgctttgc atctcttgca gacgtgacag gcaacgctgg
    89701 aactcaaact tcagccgctc gccctgataa atttcataca gcggcacttc tgcactcacc
    89761 actaatttga catggcgctc gtaaaactca tccaccagcg caataaagcg ccgcgcttcg
    89821 ctctccatca accgcgtcat aactggtaca tcaaacaaca tgaccgtgtg aaagaggcgt
    89881 gagagcgcaa tatagtcatg ctgactgcgg gcgtcgacgc acagcgtagt aaaagaaacc
    89941 gccagcgtct ggttctcgac gcccattgtc gccaatggcc gatggttgat ttctaacgtc
    90001 ggtgaatttt ctcgtttcgc ccccgccagc gccaaccata gtttatccat ttgcgcccgg
    90061 gtttcatcgt tgaggggcga aagccacaga tgcgcctgag tgagtgtacg cagtcgataa
    90121 tcaacaccag cgtccacgtt cattacatca caatgctgtt taatggcatc gattgcaggc
    90181 aaaaaacgcg cacgttgcag gccatttcga taaagttcgt ccggcggaat atttgacgtc
    90241 gctaccaggg taatacctcg ggcgaacagg gctttcatca gaccgccaag tagcatggca
    90301 tcggtaatat cagaaacaaa aaattcgtca aaacagagca cgtcagtttc tgctttaaag
    90361 cgatcggcaa taatttccag cggatcgctt tggccttgta attcggtcag ctcttcgtgc
    90421 acccgcagca taaaacggtg aaagtgcaga cgctgtttcc gctctcccgg caggctttga
    90481 tagaaaaggt ccatcagcca ggttttcccg cgtcctacac cgccccacat atataagcca
    90541 cgcactggcg tatgctttgt gtcttcgcgt ttaccccaca gcttaccgac ccgcgccatt
    90601 agcccactcg tcctgggggc tggaggcgtg ctattgatga gttcctgata aataatttcc
    90661 aggcggctga cggcctcttt ttgaacgtcg tcgggttgat ggctgccttc attgagcgcc
    90721 ttcaggtatt gcgatgttgg ggtaacgctt tgcatgatct tattgttatt ccttgaataa
    90781 tcggtgcgcc gttgttcacg gttgacgaaa aaaaggccgt tctacactac gcgatatgca
    90841 gtcgggattc cacttctgtg gaattaacgg ttatagtggc ataatcagcc gcaggcatgg
    90901 agcctgaagc caacacccta cggaaacaaa agacaacggg agatgttcat gacctgggaa
    90961 tatgcgctaa ttgggttagt cgtcggcatc attattggtg ctgtggccat gcgttttggt
    91021 aatcgtaaac tacgccagca acaggcgttg cagtacgaac tggaaaagaa taaagctgaa
    91081 ctggacgagt atcgcgaaga gctggttagc cactttgccc gcagcgcgga attactggat
    91141 accatggcgc acgactatcg ccagctgtat cagcacatgg cgaaaagctc cagtagcctg
    91201 ctgccggaac tgtctgcgga agcaaacccg ttccgtaatc gtctggcaga gtctgaagcc
    91261 agcaacgatc aggcaccggt gcagatgcct cgcgactatt ctgaaggcgc atccggcctg
    91321 ctgcgtactg gcgcgaagcg cgactaattt atttttcggg cgcagccatt gcgccctcct
    91381 cttctctccc tccccgacta tcatttaatc tggtgtctca ttgttagccg tctgaaaatt
    91441 caataacatc aaactgtttt gaatctcttt tcttatcatt caggtacgag agcaggaata
    91501 atgaaaaaac aaacccagct gttgagtgca ttagcgttaa gtgtcgggtt aactctctcg
    91561 gcgtcatttc aggccgtcgc gtcgattcca ggccaggttg ccgatcaggc ccccctcccc
    91621 agtctggctc caatgctgga aaaagtgctt ccggcagtgg tgagcgtacg ggtggaagga
    91681 acggccagtc agggacagaa aatcccggaa gaattcaaaa agttttttgg tgatgattta
    91741 ccggatcaac ctgcacaacc cttcgaaggt ttaggctccg gtgtcatcat caacgccaat
    91801 aaaggctatg tgctgaccaa caaccatgtg attaatcagg cacagaaaat cagtattcag
    91861 ctcaatgatg ggcgcgagtt tgatgcaaaa ctgattggta gcgatgacca gagcgatatc
    91921 gccctgttgc aaattcaaaa cccgagcaaa ttaacgcaaa tcgctattgc cgactccgat
    91981 aaattgcgcg tcggtgattt tgccgtggcg gtcggtaacc catttggcct tgggcaaacc
    92041 gccacctctg gcattatatc cgcattaggc cgcagcgggt tgaatcttga aggtctggaa
    92101 aactttatcc agacagatgc ttccattaac cgcggtaact ccggcggtgc actgttaaac
    92161 cttaacggtg agttaattgg catcaacact gcaatccttg cgcctggcgg cggtagcgtc
    92221 gggattggat ttgccattcc cagtaatatg gcgcgaacac tggcgcagca gcttatcgac
    92281 tttggagaaa tcaaacgcgg cctgttaggc atcaaaggca ccgagatgag tgccgatatc
    92341 gccaaggcct tcaaccttga cgtgcagcgt ggcgcgtttg tcagcgaagt gttgccaggt
    92401 tctggctcgg cgaaagcggg cgtcaaagcg ggcgatatta ttaccagcct caacggcaaa
    92461 ccgctgaata gctttgctga gttgcgctct cgtatcgcga ccaccgagcc gggcacgaaa
    92521 gtgaagcttg gcctgctgcg taacggcaaa ccactggaag tagaagtgac gctcgatacc
    92581 agcacctctt cgtcggccag cgctgaaatg atcacgccag cgctggaagg tgcaacgttg
    92641 agcgatggtc aactaaaaga tggtggcaaa ggtattaaga tcgatgaggt tgtcaaagga
    92701 agcccagctg ctcaggctgg cttgcaaaaa gacgatgtga tcattggcgt caaccgcgat
    92761 cgggtgaact cgattgctga aatgcgtaaa gtgctggcgg caaaaccggc catcattgcc
    92821 ctgcaaattg tacgcggcaa tgaaagcctc tacctactaa tgcgttaatg tcgtaaaccg
    92881 ggcatcaggc tgacgtgtga tgtccggtta actcgtggta tgctgctgcc gttccctttt
    92941 ttaacgatgc ctccatcatg tttgtgaagc tcttacgttc cgttgcgatt ggattaattg
    93001 tcggcgctat tctgctggtt gccatgcctt cgctgcgcag ccttaacccg ctttccactc
    93061 cgcaatttga cagtaccgat gagacgcctg ccagctataa tctggcggtt cgccgcgccg
    93121 cgccagcggt ggttaacgtt tataaccgcg gtttgaacac caactctcac aaccagcttg
    93181 agatccgcac cctgggatct ggtgtaatca tggatcaacg cgggtatatc atcaccaata
    93241 aacacgtgat caacgacgcc gatcagatca tcgtcgcctt acaggatgga cgtgtttttg
    93301 aagcgttact ggttggctct gactctctaa ccgatctggc ggtgctgaaa attaacgcca
    93361 caggcggttt accaacgatt ccgatcaatc cacgccgcgt accgcacatt ggcgatgtgg
    93421 ttctggcgat cggtaacccg tacaacctcg ggcagaccat tacccagggg attatcagtg
    93481 ccacgggtag aatcggtctg aacccgaccg ggcggcaaaa cttcctgcaa accgatgctt
    93541 ccattaacca cggtaactct ggcggcgcgc tggtgaactc gctgggcgaa ctgatgggca
    93601 ttaacacgct gtcgtttgat aagagtaacg atggcgaaac gccggaaggt atcggctttg
    93661 cgattccttt ccagttagcg accaaaatta tggataaact gatccgtgat ggccgtgtga
    93721 tccgtggcta cattggcatt ggtggacgtg agatcgcccc attacatgcg cagagtggcg
    93781 gcatggatca actgcaaggg atcgtagtta acgaagtgtc gcctgatggc ccagcggcaa
    93841 atgcaggcat ccaggtcaac gatctgatta tctctgtgaa taacaaaccg gccatctctg
    93901 ctctggagac gatggatcag gtggcagaaa ttcgccctgg ttcggtgatc ccggttgtcg
    93961 tgatgcgtga cgataaacag ttaacattgc aggtcaccat tcaggaatat ccggcgacca
    94021 actaaggtca gcgtaaaaaa acaaaaaccg gagtctgtgc tccggttttt tattatccgc
    94081 taatcaatta cttattaacg aactcttcgc ccagggcgat atctttcttc agcgtatcca
    94141 gcataccttc cagcgcgttc tgttcaaatg cgctcagagt accgatagat ttacgctctt
    94201 ccacgccgtt tttacccagc agcagcggtt gagagaagaa acgagcgtac tgaccgtcgc
    94261 cttcaacgta ggcacattcg acaacgcctt gttcgccctg cagtgcgcga accagagaca
    94321 gaccaaaacg tgcagctgcc tggcccatag acagggttgc agacccgcca ccggctttcg
    94381 cttcaaccac ctcagtaccc gcgttctgga tacgtttggt cagatcagcc acttcctgct
    94441 cggtaaaact aacgccagga acctgtgaca gcagcggcag aatggtaaca ccagagtgac
    94501 caccaataac cggcacttca acttcgcctg gctgtttgcc tttcagttcc gcaacaaagg
    94561 tgttggaacg aatgatatcc agcgtggtaa cgccgaacag tttgtttttg tcataaacac
    94621 cggctttttt cagcacttca gcagcaatcg caactgtagt gttaaccggg ttagtaataa
    94681 taccaatgca cgctttcggg caggttttcg caacttgctg taccaggttt ttcacgatgc
    94741 cggcgttaac gttaaacagg tcggaacgat ccatacccgg tttacgcgct acgcctgcag
    94801 agataagaac gacatctgca ccttccagcg ccggagtcgc atcttcacct gaaaaacctt
    94861 tgattttcac agcagtaggg atatggctca gatcgacagc cacaccggga gtcactggag
    94921 cgatatcata cagagagagt tctgaacctg aaggcagttg ggtttttaac agtagtgcaa
    94981 gcgcctggcc aataccgcca gcagcgccga ggactgcgac tttcatccta aactccttat
    95041 tatattgata aactaagata tgttgctccg ctgccgcgac cttaatccac aaaattgccg
    95101 tttacaatga ccacatctca agaatgtgta gtcacgcaag tttagcgttt atgcatttaa
    95161 ttgccgtaat caggaaccta acttacgtaa ttaacagtca cttatgcatt agcgcaacat
    95221 tccaacaggt ggtgacaata taccctaccg ctcagccaaa acaatatcaa tttgataaca
    95281 attaatttac ttttaagcag aatttgcagg ccgtgacgca ggcatgtttc tcaataacga
    95341 aatttgataa aatcccgctc tttcataaca ttatttcagc cttcttcagg gctgactgtt
    95401 tgcataaaaa ttcatctgta tgcacaataa tgttgtatca accaccatat cgggtgactt
    95461 atgcgaagct cggctaagca agaagaacta gttaaagcat ttaaagcatt acttaaagaa
    95521 gagaaattta gctcccaggg cgaaatcgtc gccgcgttgc aggagcaagg ctttgacaat
    95581 attaatcagt ctaaagtctc gcggatgttg accaagtttg gtgctgtacg tacacgcaat
    95641 gccaaaatgg aaatggttta ctgcctgcca gctgaactgg gtgtaccaac cacctccagt
    95701 ccattgaaga atctggtact ggatatcgac tacaacgatg cagttgtcgt gattcatacc
    95761 agccctggtg cggcgcagtt aattgctcgc ctgctggact cactgggcaa agcagaaggt
    95821 attctgggca ccatcgctgg cgatgacacc atctttacta cccctgctaa cggtttcacc
    95881 gtcaaagagc tgtacgaagc gattttagag ctgttcgacc aggagcttta atctctgccc
    95941 cgtcgcttct ggcggcgggg aaaatgttgc ttttgcctat caacctcctg ctttcccctg
    96001 cgattcattt aacgaatagt gcgttttact gcgacatatc at
//
LOCUS       DAZFJL010000002        87612 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFJL010000002 DAZFJL010000000
VERSION     DAZFJL010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 87612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.002"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            53..280
                     /locus_tag="ACHJ8C_000100"
     CDS             53..280
                     /locus_tag="ACHJ8C_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5550031.1"
                     /translation="MACNVFAFSISGGGSERSYKETEKTSAMTTTHSTKLQPSQAILF
                     KMREDAPPLNLTEETTPPFPTKANYLIHPVR"
     gene            complement(357..599)
                     /gene="yfdY"
                     /locus_tag="ACHJ8C_000101"
     CDS             complement(357..599)
                     /gene="yfdY"
                     /locus_tag="ACHJ8C_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO5550032.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            952..1872
                     /gene="lpxP"
                     /locus_tag="ACHJ8C_000102"
     CDS             952..1872
                     /gene="lpxP"
                     /locus_tag="ACHJ8C_000102"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO5550033.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPADENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2228..2299
                     /gene="ypdK"
                     /locus_tag="ACHJ8C_000103"
     CDS             2228..2299
                     /gene="ypdK"
                     /locus_tag="ACHJ8C_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO5550034.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2363..3601)
                     /gene="alaC"
                     /locus_tag="ACHJ8C_000104"
     CDS             complement(2363..3601)
                     /gene="alaC"
                     /locus_tag="ACHJ8C_000104"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO5550035.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            3978..5675
                     /gene="ypdA"
                     /locus_tag="ACHJ8C_000105"
     CDS             3978..5675
                     /gene="ypdA"
                     /locus_tag="ACHJ8C_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO5550036.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAMTNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            5690..6424
                     /gene="ypdB"
                     /locus_tag="ACHJ8C_000106"
     CDS             5690..6424
                     /gene="ypdB"
                     /locus_tag="ACHJ8C_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO5550037.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            6437..7294
                     /gene="ypdC"
                     /locus_tag="ACHJ8C_000107"
     CDS             6437..7294
                     /gene="ypdC"
                     /locus_tag="ACHJ8C_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5550038.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYSNLLEAKQQRMVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQSIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWIQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(7297..9792)
                     /gene="ptsP"
                     /locus_tag="ACHJ8C_000108"
     CDS             complement(7297..9792)
                     /gene="ptsP"
                     /locus_tag="ACHJ8C_000108"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO5550039.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTLLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRHLMAEQHQGLGAAIISNMEQ
                     ICAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDKKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGELQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTAAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFAPEED
                     VRPLLALENIFVDQSFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9817..10854)
                     /gene="ypdE"
                     /locus_tag="ACHJ8C_000109"
     CDS             complement(9817..10854)
                     /gene="ypdE"
                     /locus_tag="ACHJ8C_000109"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO5550040.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILREEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSCDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQSD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(10854..11939)
                     /gene="ypdF"
                     /locus_tag="ACHJ8C_000110"
     CDS             complement(10854..11939)
                     /gene="ypdF"
                     /locus_tag="ACHJ8C_000110"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO5550041.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDAMHTLATIVRQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     CEIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISVIRPGVRCQQVDDAARRV
                     ITEAGYGKFFGHNTGHAIGIEVHEEPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTLQGAEVLYAMPKTVLLTGEA"
     gene            complement(11954..13201)
                     /gene="fryC"
                     /locus_tag="ACHJ8C_000111"
     CDS             complement(11954..13201)
                     /gene="fryC"
                     /locus_tag="ACHJ8C_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO5550042.1"
                     /translation="MAIKKRSATVVHGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIESL"
     gene            complement(13223..13549)
                     /gene="fryB"
                     /locus_tag="ACHJ8C_000112"
     CDS             complement(13223..13549)
                     /gene="fryB"
                     /locus_tag="ACHJ8C_000112"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO5550043.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(13768..14733)
                     /gene="glk"
                     /locus_tag="ACHJ8C_000113"
     CDS             complement(13768..14733)
                     /gene="glk"
                     /locus_tag="ACHJ8C_000113"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO5550044.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEGIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            14937..16193
                     /gene="yfeO"
                     /locus_tag="ACHJ8C_000114"
     CDS             14937..16193
                     /gene="yfeO"
                     /locus_tag="ACHJ8C_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO5550045.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIMVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPIPPSALPG
                     LIVALILGLAGGVSLGPEHPIITVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSNEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVFVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            16308..16634
                     /gene="ypeC"
                     /locus_tag="ACHJ8C_000115"
     CDS             16308..16634
                     /gene="ypeC"
                     /locus_tag="ACHJ8C_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO5550046.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(16776..18014)
                     /gene="mntH"
                     /locus_tag="ACHJ8C_000116"
     CDS             complement(16776..18014)
                     /gene="mntH"
                     /locus_tag="ACHJ8C_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO5550047.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            18350..19552
                     /gene="nupC"
                     /locus_tag="ACHJ8C_000117"
     CDS             18350..19552
                     /gene="nupC"
                     /locus_tag="ACHJ8C_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO5550048.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(19602..21791)
                     /gene="pdeA"
                     /locus_tag="ACHJ8C_000118"
     CDS             complement(19602..21791)
                     /gene="pdeA"
                     /locus_tag="ACHJ8C_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO5550049.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRLISPLAVIHSNYIFL
                     AWMPLCVMLSILFIFGWRGVVPILCGMFCTNLWNLHLSFLQTAVMIGSQAFAVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVAPIGIKCSMYLVGNFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVMLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYYISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETPQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(21998..22073)
                     /locus_tag="ACHJ8C_000119"
     tRNA            complement(21998..22073)
                     /locus_tag="ACHJ8C_000119"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22038..22040),aa:Ala,seq:ggc)
     gene            complement(22113..22188)
                     /locus_tag="ACHJ8C_000120"
     tRNA            complement(22113..22188)
                     /locus_tag="ACHJ8C_000120"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22153..22155),aa:Ala,seq:ggc)
     gene            22409..22768
                     /gene="yfeC"
                     /locus_tag="ACHJ8C_000121"
     CDS             22409..22768
                     /gene="yfeC"
                     /locus_tag="ACHJ8C_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO5550050.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            22770..23162
                     /gene="yfeD"
                     /locus_tag="ACHJ8C_000122"
     CDS             22770..23162
                     /gene="yfeD"
                     /locus_tag="ACHJ8C_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO5550051.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(23202..24410)
                     /gene="insQ"
                     /locus_tag="ACHJ8C_000123"
     CDS             complement(23202..24410)
                     /gene="insQ"
                     /locus_tag="ACHJ8C_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO5550052.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKNAPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNDSFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWH
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRRVKFSNNWQKQKRKIQCLHSRIASIRRDYLHKVTTTISKNHAMTVIENLKVSNMSK
                     SAAGTVSQPGRHVRAKSGLNRPIQEQGWYEMRRQLEYKALWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            24418..24842
                     /gene="tnpA"
                     /locus_tag="ACHJ8C_000124"
                     /pseudo
     CDS             24418..24842
                     /gene="tnpA"
                     /locus_tag="ACHJ8C_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(24960..26375)
                     /gene="gltX"
                     /locus_tag="ACHJ8C_000125"
     CDS             complement(24960..26375)
                     /gene="gltX"
                     /locus_tag="ACHJ8C_000125"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO5550053.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLNLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLTAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            26634..26709
                     /locus_tag="ACHJ8C_000126"
     tRNA            26634..26709
                     /locus_tag="ACHJ8C_000126"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26667..26669,aa:Val,seq:tac)
     gene            26754..26829
                     /locus_tag="ACHJ8C_000127"
     tRNA            26754..26829
                     /locus_tag="ACHJ8C_000127"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26787..26789,aa:Val,seq:tac)
     gene            26876..26951
                     /locus_tag="ACHJ8C_000128"
     tRNA            26876..26951
                     /locus_tag="ACHJ8C_000128"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26909..26911,aa:Val,seq:tac)
     gene            26956..27031
                     /locus_tag="ACHJ8C_000129"
     tRNA            26956..27031
                     /locus_tag="ACHJ8C_000129"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26989..26991,aa:Lys,seq:ttt)
     gene            complement(27142..28026)
                     /gene="xapR"
                     /locus_tag="ACHJ8C_000130"
     CDS             complement(27142..28026)
                     /gene="xapR"
                     /locus_tag="ACHJ8C_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFO5550054.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVY
                     ETQQVTPALVKLLAALAQ"
     gene            complement(28067..28225)
                     /locus_tag="ACHJ8C_000131"
     CDS             complement(28067..28225)
                     /locus_tag="ACHJ8C_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFO5550055.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(28278..29534)
                     /gene="xapB"
                     /locus_tag="ACHJ8C_000132"
     CDS             complement(28278..29534)
                     /gene="xapB"
                     /locus_tag="ACHJ8C_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFO5550056.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            complement(29595..30428)
                     /gene="xapA"
                     /locus_tag="ACHJ8C_000133"
     CDS             complement(29595..30428)
                     /gene="xapA"
                     /locus_tag="ACHJ8C_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFO5550057.1"
                     /translation="MYQAQFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGVPVACMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            30676..31440
                     /gene="yfeN"
                     /locus_tag="ACHJ8C_000134"
     CDS             30676..31440
                     /gene="yfeN"
                     /locus_tag="ACHJ8C_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO5550058.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(31479..32405)
                     /gene="yfeR"
                     /locus_tag="ACHJ8C_000135"
     CDS             complement(31479..32405)
                     /gene="yfeR"
                     /locus_tag="ACHJ8C_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5550059.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRCYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            32495..33493
                     /gene="yfeH"
                     /locus_tag="ACHJ8C_000136"
     CDS             32495..33493
                     /gene="yfeH"
                     /locus_tag="ACHJ8C_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO5550060.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(33490..33708)
                     /gene="ypeB"
                     /locus_tag="ACHJ8C_000137"
     CDS             complement(33490..33708)
                     /gene="ypeB"
                     /locus_tag="ACHJ8C_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO5550061.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(33710..35725)
                     /gene="ligA"
                     /locus_tag="ACHJ8C_000138"
     CDS             complement(33710..35725)
                     /gene="ligA"
                     /locus_tag="ACHJ8C_000138"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO5550062.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLMQFKAWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAVKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPEPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDETEMLRLLGS"
     gene            complement(35796..36794)
                     /gene="zipA"
                     /locus_tag="ACHJ8C_000139"
     CDS             complement(35796..36794)
                     /gene="zipA"
                     /locus_tag="ACHJ8C_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO5550063.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPTQPVQQPVQQPAYQPQPEQPLQQPVSPQVASAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            37024..37785
                     /gene="cysZ"
                     /locus_tag="ACHJ8C_000140"
     CDS             37024..37785
                     /gene="cysZ"
                     /locus_tag="ACHJ8C_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO5550064.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            37970..38941
                     /gene="cysK"
                     /locus_tag="ACHJ8C_000141"
     CDS             37970..38941
                     /gene="cysK"
                     /locus_tag="ACHJ8C_000141"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO5550065.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            39325..39582
                     /gene="ptsH"
                     /locus_tag="ACHJ8C_000142"
     CDS             39325..39582
                     /gene="ptsH"
                     /locus_tag="ACHJ8C_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO5550066.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            39627..41354
                     /gene="ptsI"
                     /locus_tag="ACHJ8C_000143"
     CDS             39627..41354
                     /gene="ptsI"
                     /locus_tag="ACHJ8C_000143"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO5550067.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            41395..41904
                     /gene="crr"
                     /locus_tag="ACHJ8C_000144"
     CDS             41395..41904
                     /gene="crr"
                     /locus_tag="ACHJ8C_000144"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO5550068.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(41946..42797)
                     /gene="pdxK"
                     /locus_tag="ACHJ8C_000145"
                     /gene_synonym="thiJ"
     CDS             complement(41946..42797)
                     /gene="pdxK"
                     /locus_tag="ACHJ8C_000145"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO5550069.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAAHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            42902..43270
                     /gene="yfeK"
                     /locus_tag="ACHJ8C_000146"
     CDS             42902..43270
                     /gene="yfeK"
                     /locus_tag="ACHJ8C_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO5550070.1"
                     /translation="MKKIICLVVTLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(43273..44184)
                     /gene="cysM"
                     /locus_tag="ACHJ8C_000147"
     CDS             complement(43273..44184)
                     /gene="cysM"
                     /locus_tag="ACHJ8C_000147"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO5550071.1"
                     /translation="MSTLEQTIGNTPLVKLQRMAPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(44319..45416)
                     /gene="cysA"
                     /locus_tag="ACHJ8C_000148"
     CDS             complement(44319..45416)
                     /gene="cysA"
                     /locus_tag="ACHJ8C_000148"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO5550072.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGD
                     RLYVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(45406..46281)
                     /gene="cysW"
                     /locus_tag="ACHJ8C_000149"
     CDS             complement(45406..46281)
                     /gene="cysW"
                     /locus_tag="ACHJ8C_000149"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO5550073.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(46281..47114)
                     /gene="cysT"
                     /locus_tag="ACHJ8C_000150"
     CDS             complement(46281..47114)
                     /gene="cysT"
                     /locus_tag="ACHJ8C_000150"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO5550074.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFRKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(47114..48130)
                     /gene="cysP"
                     /locus_tag="ACHJ8C_000151"
     CDS             complement(47114..48130)
                     /gene="cysP"
                     /locus_tag="ACHJ8C_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO5550075.1"
                     /translation="MAVNLLKKNSLVLVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMGKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(48288..49079)
                     /gene="ucpA"
                     /locus_tag="ACHJ8C_000152"
     CDS             complement(48288..49079)
                     /gene="ucpA"
                     /locus_tag="ACHJ8C_000152"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO5550076.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            49359..50255
                     /gene="murQ"
                     /locus_tag="ACHJ8C_000153"
     CDS             49359..50255
                     /gene="murQ"
                     /locus_tag="ACHJ8C_000153"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO5550077.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHTQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSSTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKCLDQHGGFIRKALEKE"
     gene            50259..51683
                     /gene="murP"
                     /locus_tag="ACHJ8C_000154"
     CDS             50259..51683
                     /gene="murP"
                     /locus_tag="ACHJ8C_000154"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO5550078.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KTRQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIECMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVSGFIFTTLFGCRNVNLD"
     gene            51686..51772
                     /locus_tag="ACHJ8C_000155"
                     /pseudo
     CDS             51686..51772
                     /locus_tag="ACHJ8C_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(51861..52760)
                     /gene="yfeX"
                     /locus_tag="ACHJ8C_000156"
     CDS             complement(51861..52760)
                     /gene="yfeX"
                     /locus_tag="ACHJ8C_000156"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO5550079.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(52856..53431)
                     /gene="yfeY"
                     /locus_tag="ACHJ8C_000157"
     CDS             complement(52856..53431)
                     /gene="yfeY"
                     /locus_tag="ACHJ8C_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO5550080.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADSDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(53492..53941)
                     /gene="yfeZ"
                     /locus_tag="ACHJ8C_000158"
     CDS             complement(53492..53941)
                     /gene="yfeZ"
                     /locus_tag="ACHJ8C_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO5550081.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(53928..54353)
                     /gene="ypeA"
                     /locus_tag="ACHJ8C_000159"
     CDS             complement(53928..54353)
                     /gene="ypeA"
                     /locus_tag="ACHJ8C_000159"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO5550082.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            54567..55436
                     /gene="amiA"
                     /locus_tag="ACHJ8C_000160"
     CDS             54567..55436
                     /gene="amiA"
                     /locus_tag="ACHJ8C_000160"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO5550083.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNG
                     HSKPKAKKSGGRRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            55440..56339
                     /gene="hemF"
                     /locus_tag="ACHJ8C_000161"
     CDS             55440..56339
                     /gene="hemF"
                     /locus_tag="ACHJ8C_000161"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO5550084.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLSAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(56345..57397)
                     /gene="eutR"
                     /locus_tag="ACHJ8C_000162"
     CDS             complement(56345..57397)
                     /gene="eutR"
                     /locus_tag="ACHJ8C_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO5550085.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRLGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(57443..57943)
                     /gene="eutK"
                     /locus_tag="ACHJ8C_000163"
     CDS             complement(57443..57943)
                     /gene="eutK"
                     /locus_tag="ACHJ8C_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO5550086.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(57956..58615)
                     /gene="eutL"
                     /locus_tag="ACHJ8C_000164"
     CDS             complement(57956..58615)
                     /gene="eutL"
                     /locus_tag="ACHJ8C_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO5550087.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVANIENGAAFQWANDKQDTAFLAHVVSRTGSYLSSTAGITPGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(58625..59512)
                     /gene="eutC"
                     /locus_tag="ACHJ8C_000165"
     CDS             complement(58625..59512)
                     /gene="eutC"
                     /locus_tag="ACHJ8C_000165"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO5550088.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(59533..60894)
                     /gene="eutB"
                     /locus_tag="ACHJ8C_000166"
     CDS             complement(59533..60894)
                     /gene="eutB"
                     /locus_tag="ACHJ8C_000166"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO5550089.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DEISVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(60906..62309)
                     /gene="eutA"
                     /locus_tag="ACHJ8C_000167"
     CDS             complement(60906..62309)
                     /gene="eutA"
                     /locus_tag="ACHJ8C_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO5550090.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSRSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLCPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(62306..63532)
                     /gene="eutH"
                     /locus_tag="ACHJ8C_000168"
     CDS             complement(62306..63532)
                     /gene="eutH"
                     /locus_tag="ACHJ8C_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO5550091.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGA
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATAAKTEAEAQS"
     gene            complement(63949..65136)
                     /gene="eutG"
                     /locus_tag="ACHJ8C_000169"
     CDS             complement(63949..65136)
                     /gene="eutG"
                     /locus_tag="ACHJ8C_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO5550092.1"
                     /translation="MQSELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLVARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQVGRALRTKKSDDRD
                     AINAVSELIAEVGINKRLADVGATTGHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(65126..65962)
                     /gene="eutJ"
                     /locus_tag="ACHJ8C_000170"
     CDS             complement(65126..65962)
                     /gene="eutJ"
                     /locus_tag="ACHJ8C_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO5550093.1"
                     /translation="MAHDEQWLTPRLHIAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGIY
                     AK"
     gene            complement(65973..67376)
                     /gene="eutE"
                     /locus_tag="ACHJ8C_000171"
     CDS             complement(65973..67376)
                     /gene="eutE"
                     /locus_tag="ACHJ8C_000171"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO5550094.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPTAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLKVPEQTRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAC
                     TFVRLRRCVLVDAFRIV"
     gene            complement(67388..67675)
                     /gene="eutN"
                     /locus_tag="ACHJ8C_000172"
     CDS             complement(67388..67675)
                     /gene="eutN"
                     /locus_tag="ACHJ8C_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO5550095.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(67782..68075)
                     /gene="eutM"
                     /locus_tag="ACHJ8C_000173"
     CDS             complement(67782..68075)
                     /gene="eutM"
                     /locus_tag="ACHJ8C_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO5550096.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(68114..69130)
                     /gene="pta"
                     /locus_tag="ACHJ8C_000174"
     CDS             complement(68114..69130)
                     /gene="pta"
                     /locus_tag="ACHJ8C_000174"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO5550097.1"
                     /translation="MIIKRCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(69127..69930)
                     /gene="eutT"
                     /locus_tag="ACHJ8C_000175"
     CDS             complement(69127..69930)
                     /gene="eutT"
                     /locus_tag="ACHJ8C_000175"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO5550098.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(69927..70628)
                     /gene="eutQ"
                     /locus_tag="ACHJ8C_000176"
     CDS             complement(69927..70628)
                     /gene="eutQ"
                     /locus_tag="ACHJ8C_000176"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO5550099.1"
                     /translation="MKKLITANDIREAHARGELAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGALQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(70603..71082)
                     /gene="eutP"
                     /locus_tag="ACHJ8C_000177"
     CDS             complement(70603..71082)
                     /gene="eutP"
                     /locus_tag="ACHJ8C_000177"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO5550100.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVELNDNGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(71095..71430)
                     /gene="eutS"
                     /locus_tag="ACHJ8C_000178"
     CDS             complement(71095..71430)
                     /gene="eutS"
                     /locus_tag="ACHJ8C_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO5550101.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(71723..74002)
                     /gene="maeB"
                     /locus_tag="ACHJ8C_000179"
     CDS             complement(71723..74002)
                     /gene="maeB"
                     /locus_tag="ACHJ8C_000179"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO5550102.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMASGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            74291..75241
                     /gene="tal"
                     /locus_tag="ACHJ8C_000180"
     CDS             74291..75241
                     /gene="tal"
                     /locus_tag="ACHJ8C_000180"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO5550103.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPIDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            75261..77264
                     /gene="tkt"
                     /locus_tag="ACHJ8C_000181"
     CDS             75261..77264
                     /gene="tkt"
                     /locus_tag="ACHJ8C_000181"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO5550104.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVLMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVVHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWNAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVSARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(77341..78384)
                     /gene="ypfG"
                     /locus_tag="ACHJ8C_000182"
     CDS             complement(77341..78384)
                     /gene="ypfG"
                     /locus_tag="ACHJ8C_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO5550105.1"
                     /translation="MRYRIFLLFFFALLPMSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGRAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSREERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(78510..79085)
                     /gene="nudK"
                     /locus_tag="ACHJ8C_000183"
     CDS             complement(78510..79085)
                     /gene="nudK"
                     /locus_tag="ACHJ8C_000183"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO5550106.1"
                     /translation="MTQQITLVKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(79153..81132)
                     /gene="aegA"
                     /locus_tag="ACHJ8C_000184"
     CDS             complement(79153..81132)
                     /gene="aegA"
                     /locus_tag="ACHJ8C_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO5550107.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQRHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAVGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEQQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLNSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            81338..83056
                     /gene="narQ"
                     /locus_tag="ACHJ8C_000185"
     CDS             81338..83056
                     /gene="narQ"
                     /locus_tag="ACHJ8C_000185"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO5550108.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLI
                     WHPCSK"
     gene            complement(83143..86316)
                     /gene="ehaJ"
                     /locus_tag="ACHJ8C_000186"
     CDS             complement(83143..86316)
                     /gene="ehaJ"
                     /locus_tag="ACHJ8C_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin glycoprotein EhaJ"
                     /protein_id="HFO5550109.1"
                     /translation="MNRTYNIVWNAARNMYVVASELSRGDSRMKTQVRSVAAMVLLAL
                     SPLSAQAESTSEVTNAMVTGETVDASDRQHSYSSGMAISAYSGSPLDKGASLKAQVDN
                     VLELESGNAYILNGESQSFDSVSVGVGSMLDLDGGELTIGHGSRYRWVSFFEGIVTGS
                     GLLNLYSELNIRSTSDLSGLNATINIEQGAQVISETTNLGRGDIVVDGLLSIFSPAGG
                     GLSNSLSGSGEVSTVWDLTVSGDNDSFNGTWIIGANKDMDADLAVADASALGAGNIIN
                     GGTLTLGGAEDWMLNSGNVISDYDKYDDDMLVGSYVGKVVKEDANTITVAHANTYSGG
                     TDIEAGTLNADHTGALGTGDVANYATLNLNAAGQYTLTNITTFDGGTTSLAAGATLAT
                     GMLDQQDGSVLSINLGTDASSPIITAHDASLGGTLNITGIGNINNPLTHDPYAFTLID
                     ADNAINGDFDTLTVAGMEARKVDFLTVDSHVNSADDTQYELTTSLSWYADEKLAATDA
                     HGTFTLSDPNENFTLNTELVDVNPNSVTGWDGKTLTKAGDGTLTLNVANTWSGDTRVN
                     DGTLWLTDSGVIGASGSTQAVNVAEGATFGGSGVVNGDVYNSGNIAMSHGGETGNTLT
                     INGNYHGDNGSQININSQLGDDSSPTDKLVISGDTSGNTMVYVANVNGKGAQTKNGIE
                     VIDVGGQSSGTFEQGNRVQIGLYEYRLYEDGGDWYLRSQSSVPPEPDDGDCSGTDDTP
                     VTPQYRPDIGAYLGNQWMVRNLQMQTLYDREGSQYHNEDGSVWMRFKAGNAASQAADG
                     NVDINNNYSQIQLGGDILTWGNGVQSLKAGGMASYVNADTDSTSNRGADGSQFSASGN
                     VGGYNLGIYATWFADAQHHRGLYVDSWYQYGFYNNSVQNGSEGSESYDSTAQAASLEG
                     GYRYDIALENQHTLSLTPQAQVVWQRYSADSVTDNNGTRISGQDSDNWNTRLGLRVDG
                     KLHKNKTSIIQPFAEVNWLYTSGDVAVSFDDAETKQGIPSNRVQAKVGIQVNLDSQWS
                     ITGQVVGETGSHNYNDLNGSLNLRYSW"
     gene            complement(86320..87555)
                     /gene="ehaJ"
                     /locus_tag="ACHJ8C_000187"
     CDS             complement(86320..87555)
                     /gene="ehaJ"
                     /locus_tag="ACHJ8C_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-glycosylating O-heptosyltransferase
                     EhaJ"
                     /protein_id="HFO5550110.1"
                     /translation="MSNNDTLPNIIDGQTPLLFSTRPFIPTQKALHGIRFDFNDGARV
                     KLGPGQWQVNLIDDDSGNILLTCETDQGCVMSNKKYFINFRIQVFKDGETTPVLDVVQ
                     DLRDKTVIIHFPIGTLEELLGWFPYAERFRQKHQCQLECVMTEEIIELLRDQYPQITF
                     STRESIQTHSPYASYRIGLFFKGNDTHQPVDFRQVGFSRIAAYILGILPHEEPPLLKL
                     DAVRSIDEPYVCIAVQSSCQAKYWNNGYGWSQVISELKTLGYRVLCIDKEAHHGYGFV
                     WNHIPNGAEDFTGALPLQQRVDLLRHAAFFVGLSSGLSWLAWSCHIPVVLISGFTNPL
                     SEFYTPWRVFNSYGCHGCWDDMRIDFDHQDFLQCPRHKGTERQYECSRLITGQQVIRI
                     IHQLHHSLSEEHALLILKD"
BASE COUNT        20990 a        23956 c        21885 g        20781 t
ORIGIN      
        1 ttctatgaaa gtaaacttaa tactttttag cttattttta ttggtctcta ttatggcatg
       61 caatgttttt gcattttcca tttctggtgg tggaagtgag aggagctata aagagacgga
      121 aaaaacatca gcgatgacga caacgcattc tacaaaactt cagccatcac aggcaatttt
      181 gtttaagatg agagaagatg cgccaccatt aaacctcaca gaagaaacca cgcccccttt
      241 tccgacaaag gcgaattatc ttattcatcc tgtgcgatag cgcctgtcac ctggcaggtg
      301 aacaatcatg tctgatgctg atgtgaacac ctgcccaaat gagccatcct gacccactat
      361 cgcctgccat accccaccat aaagcgaatc atcaagccac aagccggggc aagaaacata
      421 aatatccccg tcaccagttc attaccgcca gttaaccaca tggtgaagta ataaatgagt
      481 gcagcgagga tcaccatgcc gaagaaagaa gagaccgccg ataccacgtt cagtacctga
      541 ccgatgtatc cgcttacgca gacaatacac agggcgagaa acatccataa ataaatcata
      601 gctcaacctc ctgccgcgct aaaaaacctg taacttcagt cactttaata gtaagacaat
      661 ccttaacctc tggcaatttt tgctaagcat caatgaaaac agcatgttaa atgcaagact
      721 gttgcgtacg gaaaaatatt tactttgcac gattaataat cagtagctga aagcagtcag
      781 ccagtgataa agtagagcca ccgcattcat gctcctcgtg gttatgtcct gactagtctt
      841 tacactcttt acaggaacca ttgtcgtaca tgatggccta atcaattgag tgtaccgctg
      901 tcgacatgga atcttctgcg atacaacaat tcgtatctac agaaggtaac tatgtttcca
      961 caatgcaaat tttcccgcga gtttctgcat cctcgctact ggctcacatg gtttgggctt
     1021 ggtgtactct ggctttgggt acagcttcct tatcctgttc tctgctttct cggcacgcgt
     1081 attggcgcaa tggcgcgacc attcctgaaa cggcgtgaat ctatcgcccg taaaaacctg
     1141 gaactttgct tcccgcagca ttctgcggaa gaacgcgaga agatgattgc cgaaaacttt
     1201 cgttcactcg gcatggcgct ggtagaaacc ggcatggcgt ggttctggcc cgacagtcgc
     1261 gtacgtaaat ggtttgatgt tgaagggttg gataacctta aacgcgcaca aatgcaaaat
     1321 cgcggcgtaa tggttgtcgg cgtccatttt atgtcgctgg aactgggcgg ccgcgtgatg
     1381 ggactgtgcc aaccaatgat ggctacctat cgtccacata ataatcagct gatggaatgg
     1441 gtgcagaccc gtgggcgcat gcgctctaac aaagcgatga tcggcagaaa taatctgcgc
     1501 ggcattgttg gtgcactgaa gaaaggtgaa gcggtatggt ttgctcccga tcaggattat
     1561 ggtcgtaaag gcagctcctt cgcgccgttc tttgcggtgg aaaatgtcgc cacaaccaat
     1621 ggtacgtatg tcctttcccg tctctctggc gcagccatgt taaccgtaac gatggtaaga
     1681 aaagcggatt acagcggata tcgtttgtac atcaccccag agatggaagg ctacccggca
     1741 gatgaaaatc aagccgctgc ctatatgaac aagattatcg aaaaagagat catgcgcgca
     1801 ccggagcagt acctctggat ccaccgtcgc tttaaaacgc gcccggtggg tgaatcgtcg
     1861 ttatacattt aacaaactgc tcttactctt tccctcctca aatcagccat aataacctca
     1921 tggtttttat ggcttttttc ttaaggtttt ttccttcgct gaatctctaa ttattaccgt
     1981 cagaaattaa actgtcgccg atatgagaca cctgtaagtt actaaaagta aataagaaaa
     2041 tgcctcttgt ctcgtttcag ttttaggcgt acattagcgc cgtctggagc agtttagctc
     2101 agaattctgg ggtgatgccc acggtaaaat actggacttt taaggtcgtt ttgcccggtg
     2161 tcaactcact gtatcaggtg taatgaagtc attcaggcgt aacagtaatt acgcggagag
     2221 atgtaaagtg aaatatttct ttatgggcat ttcttttatg gtcatcgttt gggccggtac
     2281 ttttgccctg atgatctaaa agcaaactgt gcaatgaaaa acaggagcca tcaggctcct
     2341 gtttgcattt ctggcgaagg cgttattccg cgttttcgtg aatatgtttg ctgctggcgg
     2401 gtagtaaacc gtcggcacgg aacatcgctt taatcccacg aatcgcctga cggatacggt
     2461 cgcggttttc aatcagcgca aagcgcacat gcgtgtcgcc gtagtcgcca aagccaatcc
     2521 ctggagagac acagaccttc gcttcgttaa gcaatttctt ggcaaattcc agcgagccca
     2581 tggcagcata cggttccggg attttcgccc agacatacat cgaagccttc ggcatttcga
     2641 ccatccagcc cgcttcatgc agccctttca ccagcacatc gcggcggcgt ttgtactgtt
     2701 cggcaatatc gcgcacgcac tgttgatcgc cctccagcgc cgcaatagcc gccacctgca
     2761 acggcgtaaa ggtgccgtaa tcgtgatagc ttttaatacg tgccaacgcg ctgaccagcg
     2821 ttttgttacc gaccataaag ccgatacgcc agcccgccat gttgtagctt ttcgacagcg
     2881 taaagaactc gaccgccacg tcgcgtgcac ctggcacctg catgattgac ggcgctttcc
     2941 agccatcgta gacgatatcg gcataggcca ggtcgtggac caccagcaca tcgtagcgtt
     3001 tagccagcgc cacaaccttc tcaaagaatt ccagttccac gcattgcgcg gttgggttcg
     3061 acgggaagcc gaggatcatc atcttcggtt tcggataact ttcgcgaatg gcgcgttcca
     3121 gttcgttgaa gaaatcgaca ccttccacca gcggcactga gcgtacctgc gccccggcaa
     3181 tcaccgcgcc gtaaatatga atggggtaac ttggattcgg caccagcact gtgtcaccgt
     3241 gatccagtgt cgccagcatc agatgcgcca ggccctcttt cgaaccaata gtgacgatgg
     3301 cttctgattc cgggtcgatt tcaacgtcgt agcgatcctg ataccagcgg gaaatggcgc
     3361 gacgtaaccg cggaatgccg cgtgaagtgg agtaaccatg cgtgtccggg cgctgggcca
     3421 ccgtacataa tttttcgacg atatgcggcg gagtcgcacc gtccgggtta cccatactga
     3481 aatcgataat atcttcgccg cgccgacgcg cagccatttt cagttcagcg gtaatgttaa
     3541 aaacgtaggg cgggagacga tcaatacgcg taaagcgacg ttcagggcga gtgtcagcca
     3601 taatttcctc agattaacgt tagcgcccgg accgtccgag cgacgtcgcc acgatggtgg
     3661 ctgttttgaa aatagcctga ttaatttctg tctgtccagc gttttagaac atttttatca
     3721 atggctttaa atgaggaact ccacttcaaa aacaggaaat ttatctacgc taacaagaaa
     3781 attaccactc attttattaa cattataatt tcaataaatt taccaaccag atgtgcatta
     3841 tcaagagatg cattgctaat acaccattta tctttcaatc cccttttaaa attgtggttt
     3901 ttcctcaaaa acggcctgct ggtcatcagc cttcaggtta cctatcatag aggtttaatc
     3961 cttattcaga gtcacccgtg cacgaaatat tcaacatgct gctggcggtc ttcgatcggg
     4021 cagcgttaat gcttatctgc ctgttctttc tcatccgtat ccgcctgttt cgcgaactgt
     4081 tgcacaagtc ggcgcactcc ccaaaagagt tgctcgccgt taccgccatt ttctcgctgt
     4141 tcgccctgtt cagcacctgg tccggcgttc ccgtagaagg ctcgctggtg aacgtacgta
     4201 ttatcgcggt gatgtcaggc gggattctgt ttggcccgtg ggtgggcatc attaccggcg
     4261 tgattgcggg tattcaccgt tatttaattg atatcggcgg cgtgacggcg atcccctgct
     4321 ttatcaccag cattctggcg ggttgtatat cgggctggat caacctgaaa atccccaaag
     4381 cacagcgctg gcgcgtcggt attctcggcg gcatgttgtg tgaaacgttg accatgattc
     4441 tggtgattgt ctgggcacca actaccgcat tggggatcga tatcgtctct aaaatcggga
     4501 ttccaatgat cctcggcagc gtctgtatcg gctttattgt gttgctggta caaagtgttg
     4561 agggagaaaa agaggccagc gccgcgcggc aggccaagct ggcgctggat atcgccaaca
     4621 aaacgctacc gctgtttcgc catgtcaata gcgagtcatt acgcaaagtc tgcgaaatta
     4681 tccgcgatga cattcacgcc gatgcggtgg cgatgactaa taccgatcat gtgctggcct
     4741 acgttggcgt gggtgaacat aactatcaga atggcgatga cttcattagc ccgactaccc
     4801 gccaggcgat gaattatgga aaaatcatca ttaaaaacaa tgatgaagcc caccgcacac
     4861 cagagattca ttccatgctg gtgatcccat tgtgggagaa aggcgttgtg accggaacgc
     4921 tgaaaattta ctactgccac gcgcatcaga taacctcgtc attacaggaa atggcggtcg
     4981 gtctgtcaca aatcatctcc acgcaactgg aggtgtcacg cgccgagcag ctgcgtgaaa
     5041 tggcaaataa ggcagagctt cgcgccctgc aaagcaaaat taatccccat ttcctgttta
     5101 acgctttgaa cgctatttca tcgtcaatcc gtctgaatcc ggatactgct cgccagctga
     5161 tctttaattt gtcgcgctat ctgcgctata acattgaatt aaaagacgat gagcaaatcg
     5221 atatcaaaaa agagctgtat caaattaaag actatattgc cattgagcag gcccgctttg
     5281 gcgacaagct gacggttatc tatgatattg atgaagaggt gaattgctgc attcccagcc
     5341 tgctgatcca gccgttggtg gaaaacgcca ttgtccacgg tatccagcct tgcaaaggta
     5401 aaggcgttgt caccatcagc gttgctgagt gcggtaatcg ggtacgcatt gcggtgcgag
     5461 ataccgggca cggcatcgat ccaaaggtga ttgagcgggt cgaagcgaac gaaatgccgg
     5521 gcaataaaat tggcctgctg aatgtccatc atcgcgtgaa gttattgtat ggcgaggggc
     5581 tgcatatccg ccgcctggaa ccaggaacgg aaattgcgtt ttacattcct aaccaacgca
     5641 ccccagtcgc ctcacaggct accttattgc tttgagccgg agtgatattg tgaaagtcat
     5701 cattgttgaa gacgaattcc tggcacaaca ggaactgagc tggctaatta aagagcacag
     5761 ccagatggag attgtcggca cctttgacga cggtctggac gtgttgaagt ttttgcagca
     5821 taaccgcgtc gacgccattt ttctggatat caatattccg tcgctggatg gcgtgttgct
     5881 ggcgcaaaac atcagccagt tcgcccataa accatttatt gtgttcatca ctgcgtggaa
     5941 agaacatgcg gtagaagcgt ttgaactgga agcgtttgac tacattctca aaccgtatca
     6001 ggagtcacgc attaccggga tgctgcaaaa actggaagca gcctggcaac aacagcagac
     6061 cagcagtacg cctgccgcga cggtaacgcg tgagaatgac accattaatc tggtgaaaga
     6121 tgagcgaatc atcgtcacgc caattaacga tatctattac gccgaagcgc acgagaaaat
     6181 gacctttgtc tatacgcggc gtgaatccta cgtaatgccg atgaacatta ccgagttttg
     6241 cagcaagctg ccgccgtcgc attttttccg ctgccatcgc tcattttgtg tcaatctgaa
     6301 caaaatacgc gaaatcgaac catggtttaa taacacctac attctgcggc tgaaagatct
     6361 ggattttgaa gtgccggtca gccgtagcaa agttaaagaa ttccgccagt taatgcatct
     6421 gtaatcggga actttcatga aagcaccagg cttgcccgcc gatcaacaat ttttcgccga
     6481 tctgttcagc ggcctggtgc ttaacccgca actccttggg cgcgtctggt ttgccagcca
     6541 gcccgcctca ttgccggtgg gcagtttatg tattgatttt ccccgtctgg atatcgtgct
     6601 acgcggcgaa tacagcaatc tgctggaagc aaagcagcaa cgtatggtgg aaggagaaat
     6661 gctgtttatt ccggcacgcg cggctaattt accggtcaac aacaaaccgg tgatgctgtt
     6721 aagcctggtg ttcgcaccga cctggcttgg gttatcgttt tacgatagcc gcaccacgtc
     6781 gttgttacat cctgctcgcc agatacagct tcccagcctg caacgcggtg aaggtgaagc
     6841 gatgcttacc gccctcaccc atcttagccg ttcgccgctg gagcagagta tcattcagcc
     6901 gctggtgtta agtttgctgc atctttgccg taacgtggtg aatatgccgc cgggcaattc
     6961 gcaaccgcgc ggcgattttc tctatcacag catttgtaac tggattcagg ataattatgc
     7021 ccagccgctc acccgcgaga gcgtggcgca gttttttaat atcacgccaa atcatctgtc
     7081 aaaactgttt gctcagcatg gaacaatgcg ttttatcgag tatgttcgct gggtacgaat
     7141 ggcaaaagcg aggatgattt tgcaaaaata tcatctgtcg attcatgaag tggcgcagcg
     7201 ttgcgggttt ccggatagcg actatttttg tcgcgttttc cggcgtcagt ttggtctgac
     7261 gccgggagag tatagcgcac gttttcaggg ctaacgtcag aaggttaatt ccgtttccag
     7321 cagcgtcagg atgctttgcg catcttgcgc ggcgaacagc gactggcgga agtttttatt
     7381 caccagttta cgcgccagct gcgagaagac tttcacatga ttcatccctt cgttagcgcc
     7441 cagtgtcagc atgatcacca gttcgacttc gcccatttct gactgccagt caaccggttt
     7501 cgccagccgg gcaatgctga tactggaatg acggatccac tgagatttgg tgtgcgggat
     7561 cgccacgcca aaaccaacgc cagtggtaac aatctcttcc cgctgccaga catcctcttc
     7621 cagctcgaac ggatgttcag tacgcccgtt aacaccgagg ttaccgcaca ggaactggat
     7681 cgcctgctct ttattactaa atgactgatc aacaaagata ttctctagtg ccagcagtgg
     7741 gcgaacgtct tcttccggcg caaacgcggt aagtaacgct tcaatttcct gcgcgctgcg
     7801 gcactcgcac gcctgacgcg ccagttcccg acacgcctcg ctatccagtt gacgaagctg
     7861 gcttttcact gccggaatac gcgggctact catgcttagc tcatccaggc ccagccctag
     7921 cagtagcggc agataacgac tttcaccgcc cagttcaccg caaataccta cccatttgcc
     7981 gcgctgatgc gcagcggtaa caatctgctg caacatgcgc aggaacgatg gcgtaatcgg
     8041 gttatacagc ggcgatacgc gcgggttatt acgatcgact gcatacagat actgggtcat
     8101 atcgttagaa ccgatactga agaaatcgac ctcatcgcag aagtggtcga tgatgtagca
     8161 caccgacggc acttccacca tgatacccag cgtaatcgtc tctgcatgac gcaggccatc
     8221 gcgcttaagc tcaacgatcg ctttttggag ctcacccttc acccataaga tctgatcgag
     8281 actgtggacc attgggatca tcaactgggc gttgccgaaa ctggcagcgc gcaaaatcgc
     8341 ccgcaattga gtacggaaca ggccagcaaa ttccgggtaa atacgtaccg cgcgatagcc
     8401 gaggaacggg ttctcttcct ggggaatgtt gaggtaagga atgcttttat cgccaccgat
     8461 gtccatcgtg cggaagataa tcggcttgtc gcccgccgcc agtagcacct gctggtacgc
     8521 ttcaaactgt tcctgctcgt ccggcgcgct gtcgcgatcc atatagagca tttcggtacg
     8581 aaacagccca acgccttccg ctccgttggc aaacgcgcct ggggcttcca gagctgtgcc
     8641 aatattcgcc gcaatatcaa tacgcttttt atcacgggaa taggccagct gcgcggcagc
     8701 ttgcgcctgt tgtttttggc gtttatccgc cagcgtctgc gccacctggt aataaccgct
     8761 caccgcatcg ttgggattaa ttgccagtac gccgcactgg gcatcaagca ctgcaggttg
     8821 cccggcataa cgggcaatcg catccagcgg caggccgctc agtaccggga tcgccgaagc
     8881 gcgggccaga atcagggtat gcgaggtgcg cccggttttc tccagaatca tgcccgcaag
     8941 attttttaaa tcgaggctca aaaactggct tggggttaaa tcttcagcca ccagaatggt
     9001 cggtttttca agcaccagat tattgcgcgg ttttagttcc ggccaggtga tatgcagcaa
     9061 ctgttcgcta atatcgcgaa tgtcgctgac ccgctcgcgc agataatcgc tggcagaagc
     9121 agaaagtttg gcacaaatct gctccatatt gctgatgatc gccgccccca gcccctgatg
     9181 ctgttctgcc atcaggtgac ggatattgcc tgcaaattca tcatcctgaa tcagcgacaa
     9241 atgggcgctg aggatagttt tactttcgcc gtcacgctca cgcaattgct ggttcagttg
     9301 ctcggcaagc gttgccagac tgtgctccag cagggtggaa tcttgcgcac tggccggtat
     9361 tgcccgataa ctgtcgaggc tgtcgctctg taacagggtc agtgtaccca cgccaacgcc
     9421 gcttgccagc acgttgccgt acagtaaatc cgggttcagg cggcttaatg aacgcggcag
     9481 cggatgcgcc gtcagttctg cctgcgtcgg ctggacgcta tcgctgtcga taaagcgcac
     9541 ctggatatac tcttccagca ctcgccgcgc ctgctcttca tcgctaccgc taatgttcag
     9601 gctgcaactg tcattaaata aagtgccagt gccaatcagc gccagcgagc ttttcgcatc
     9661 ggcctttgcg ttctggcgat ggttaataaa agtgatttcg ctttgccact ggctgcactg
     9721 ctctttaagt tcccacgccg gacgagcatg tagaccgtta ggcaaaggac agagaaattg
     9781 aatcgttaac ataaccgctc cgggttaatc aggagatcat ctgaaatccg tcagttgaac
     9841 aaccgtctcg cgggtaagac gttgaataag ggcagataaa agttgctcca tctgcaaaat
     9901 gtcacggcag tcggcaatcg atgcggcgca atgtccgtgg cgggttgcag gccccatcac
     9961 cactgtgggt acgccagtgc cggttaagtg caccgcccca ccgtccgtgc cgccattgct
    10021 gaacatatct gactgcaacg gtacgccaat ttctgccgcc acggtttcga tccaggcggt
    10081 aagttttggc ggcgcaatca gtgacttgtc gcttaacacc agcatcggcc cgttaccaat
    10141 ctggcgatgg ttagctgcgc cataatcaaa gtttttcgcc cagcaggcgg tatcaagcac
    10201 aatggcgaca tccggcgaca ccgcgcgggt ggcagtttgc ccgccgcgta atcccacctc
    10261 ttcgctggaa cttgccacca gccacacttc cgcaggcagt tcggcgtcgt gcagctcacg
    10321 cagtaacgtc accagcagat agcaaccgag gcggtcatca aaggctttcc ccatcacccg
    10381 ctggtgaggg agaacctgaa aagtggtatc gaaggtgacg cgatcgcctg gacgaattcc
    10441 tgcctgcatc acttcgtcac aggagcgcgc gccaatgtcc acgcgcatgg cgctgacgtc
    10501 attcccctgc cggtcgccgt caagcaggcc tggaattttg cactcttcac gggtagtgat
    10561 acgcaccgac tgaagctggc gggcagccat gcgtacattg ccaaccggca acacatcgat
    10621 cgccccttcg cgggagatgc tgcgcaccat aaatcccact tcgtccatat gcgcacagat
    10681 catcaccttc ggacctgtcg actcattgag tcggatcagc accgatccca gcccatcaaa
    10741 tcgcacttct ttttgcaggc gatccgcttc ttcccgtagg atctgccgta cttcctgttc
    10801 tgaggaggcg atcgcatctg cctcgctcaa cgcttttaag agcgataaat ccattatgcc
    10861 tctcccgtga gcaacactgt tttcggcatg gcgtagagca cttctgcgcc ttgcagggtg
    10921 accagcacga catcttcgat gcgcacgccc ccttgccctg gcaaataaat ccctggctcc
    10981 acggtcagta acatacctgg ctgtagcgtc gtggtgtccc gcggtgaaaa acgcggctct
    11041 tcatggactt caataccgat agcatgaccg gtgttatggc cgaaaaactt gccataaccg
    11101 gcctcggtaa tcacgcggcg cgcggcatcg tcaacctgct ggcagcgcac gccggggcga
    11161 attacagaga ttgccgcgag ctgtgcctgc aggacaattt gatagacgtt aaacagcggg
    11221 tgagattcgg cgctcacccc ttcgccattc accagcaagg tgcgcgtcat atcagagcag
    11281 tagccctgat acagcgcacc gaaatcgaga gtgacaaact cgcccgctgc aacaatcttg
    11341 tcgctggctt taccgtgcgg cagcgcccca cgccagccac tcgcgacaat ggtgtcaaaa
    11401 gaggcttttt ctgcgccctg ctggcgcata aaccactcca gttcagcggc tatctcgcat
    11461 tcgctcatac ccgcctgaat aaagcggcga atatgctctg cgccacgatc ggcaatcccg
    11521 caggcaaggc ggattttctc cacctcttct ggcgttttga tttgccgcag cacatccggc
    11581 gtggcgctaa ccagtttcgc attgagttcg gactgccagc ggtgcgcggt ttcccaactt
    11641 acctgctggc cttcaaaacc gagcgtttgc aactgctcat cggcaatgat ttgtctgacg
    11701 atggtggcaa gcgtgtgcat cgcgtcgagc aaatgcagct ggtaaccttg cgtgcgggct
    11761 tcaacatccg cgtaatagcg cgaatccacc agaatgtgcg cactttcacg gctaatcacc
    11821 acatagcctg atccggtgga gatccccaga tgcggctgtt tgttctgccg tgaggagaga
    11881 agcaccgcat ccagttgttg tgccttaagc cagtcgcgca gcgaagcgag taatgtcatc
    11941 tggattgtcc ttcttacagg ctctcgatta acaatttacc tttgcggaac atcatcagac
    12001 gcaagaacac caccatcagc gcagtaatga ttgctcccag cgcgatcccc gccatataga
    12061 cacccagatt agtgaccagc ggccacgccc agatagcgga ctccgggaac cattgcactg
    12121 cgcccagcca gacagcggcg gttgagccga caatcgcgcc gaccatatac gacggaatgg
    12181 cggtgatagg gctttccagt gcaaacggaa tcgcaccctc actgatcccc ataaacgcca
    12241 ggaacatcgc ggttttaccc tgcggataaa gctgggcgtt gaacaagcgt ttgccagtta
    12301 agcgacgatc gataatggtc gccagaccca gaccaatcgg cggaataacg atagcgatag
    12361 aacgtgcagt gaccggtaat acgtggtcag tggtaaagct gaaggcaacg aaacctgccg
    12421 ctttgttgat cgggccacca aggtcgattg ctgtcgccgc ggcgatcccc attgcgtaca
    12481 tcagcgcgcc cttctcacct gcagcggtca gcacggtacg gataccgccg ttgatccagc
    12541 caccaaaggg ggtgatgacg tagtacatcg ccagcatgac aaaaatggcg gaaagaatcg
    12601 gtagcaaaaa tgtggttttg aacgctaaca ggaaatccgg taactggatt ttctggttca
    12661 tccatttcac aaggtaaccg gcaacgatag aaataatcag cgcaccaatg aaggtggacg
    12721 gcaccggcga agaggtcgcc cattgcatcg tgctgggatc gaagttcagc agttgcgtcg
    12781 gctgggtaga catcaagccg ccgataaaac cagccgggaa cgccagtttg ccaccgatag
    12841 agttcgccac aaaagcagca aacatcggaa tggcaaaacc aaacaacaca ccgccgaagg
    12901 actgcgacag ccaggcaaat ttcagtaagg agaggtcgaa accggagaat ttcccgctat
    12961 taagagcatc catgatgccg atatcagcag gaattttaag ccagctataa gcaatcagct
    13021 gactgaaagc gaggatcaca ccgcccataa ttaaggtcgg caccatgcgt gaaatccctg
    13081 acatcacatg ctgcgggagt tcaccccaga agctggattt agaggcctgc ggattcttaa
    13141 ctgccgctgc cgcaccggat gcgccatgca caacggttgc actgcgtttt ttaatggcca
    13201 taacaattat ccctgtcgaa tattactgtt gttcagaggc aatcatctct tcgatttctt
    13261 tgatgattcc cgcagcgttt ttaattgcgt cctgcaaagt gatttcataa acgtcgcgtg
    13321 attcgaaacg ctcgttatct tccggggtaa ctgccacgga gtggatgatg atggtcgctt
    13381 cggcgatatc ctgcgccgtc aggcgattct ggataccgtc cgcgccctgg gtttcaattt
    13441 tcacttcata accggcttct accgccgctt cttccagcgc ctgagcggcc ataaaagtgt
    13501 gagccaggcc cattgggcag gcacataagg caatcagttt cttactcatc gttaattcct
    13561 cgttcagtga tagttgacga gtgaaattat gaacgtgcaa aacaaatcgc cgttaccaga
    13621 caaataatgc atttactgga gaattaatcc cgctgtacgg aagtgtgacg caggtcggcc
    13681 ttgtgagggg acacaaagaa aaatttaatt aactgtatga tttaaaagat tatcgggaga
    13741 gttacctccc gatataacag gaaggattta gagaatgtga cctaaggtct ggcgtaaatg
    13801 tgcaccggaa ccgagaagac ccggattgtc atggacgatg agatataccg gaatatcatg
    13861 gacatattcc ttaaagcgcc ctttatcttc aaatgcggcg cggaaaccag aggctttgaa
    13921 gaactcaaga aagcgcggca cgataccacc cgcaataaac acgccgccaa atgtcccgag
    13981 attgagcgcc agattgccgc caaaacggcc cataatgacg caaaacagcg acaacgcgcg
    14041 gcggcaatcg gtgcagctgt cagccagcgc gcgttcggta atatcttttg gcttgagatt
    14101 ttctggcagg cggttgtcgg ctttaacaat tgcgcgatac aaattcacca gcccagggcc
    14161 agaaagcacg cgttccgccg aaacatgacc aatttccgca cgcaatattt cgaggataat
    14221 gccctcttct tcactattcg gcgcaaaatc aacgtgaccg ccttctcctg gcaagcttac
    14281 ccaacgctta tcgacatgga ccagatgcgc aaccccaagc cctgttccgg caccgtaaac
    14341 cgcaataggc ttaccttcta ccggttctgc gccaccaaac tgaatcagat gctctttttt
    14401 cagcatcggg atcgccatcg atacagcagt aaaatcgtta ataatttcca gatggctaaa
    14461 accgagattc tttttcattt cggcaattga gaacgcccag gtatggttgg tcattgccac
    14521 ccagtcaccg gtaattgggc aagcgatggc aatacagccg tctttcacct cgaccttatg
    14581 ttcttcaaga taaacgcgaa tgaccgcttc gaggctgggg taatcaagcc ctgaataggt
    14641 cttagcctgc gagatttcac cactggcaat atcacacaga gcaagacgtg cgttggtgcc
    14701 gcccacatca ccgactaatg catactttgt cattcttcaa ctgctccgct aaagtcaaaa
    14761 taattctttc tcacactgta aatacctggg ggcataacaa caacgccgga aaggcaggct
    14821 ccctgtaaat atcgatctgg gtcacacaat tactttatcg tttcagcacc aattgcagcg
    14881 atgccttttt gcaagctggg caaactaagt atctgacccc gcataaggaa tagaacatgc
    14941 tccatccgcg agccagaacc atgttgttat tatcgctccc cgccgtggca attgggattg
    15001 cgtccagtct tattctgatt atggtgatga aaatcgcctc ggtattacag aatttgctct
    15061 ggcaacgact gccgggaact ctggggatag cccaggattc acctctctgg atcatcggtg
    15121 tattaacgct aacgggtatt gcggtggggt tggttatccg tttcagccag ggtcatgccg
    15181 gaccagatcc cgcctgtgaa ccgcttatcg gcgcaccgat tccgccctct gcgctaccag
    15241 gacttatcgt agcattaatt ctcggtcttg ctggcggcgt cagcctgggg ccggaacatc
    15301 cgatcataac cgtcaatatc gcccttgccg tcgccattgg cgctcgtctg ttaccgcgcg
    15361 tcaaccgaat ggagtggact attttagcct ctgccggaac catcggcgcg ctgtttggca
    15421 cacctgttgc ggcggcattg atattttcgc aaaccttaaa tggcagtaat gaagttccgc
    15481 tatgggatcg tctctttgcg ccgttaatgg cggcagcagc tggtgcactt actaccggat
    15541 tatttttcca tcctcatttt tcactgccca ttgcgcatta cggacagatg gaaatgaccg
    15601 atattctcag cggtgcaatt gtcgcggcga ttgccatcgc agcagggatg gtcgccgtat
    15661 ggtgcttacc acggttgcac gcgatgatgc atcaaatgaa aaatccggtg ttcgtgctgg
    15721 gtattggcgg atttattctc ggtattctgg gggttattgg tggaccggtt tcgctgttta
    15781 aagggctgga tgagatgcag cagatggtgg caaatcaggc tttcagcacc agcgattact
    15841 ttttgctggc ggtgataaaa cttgccgccc tggtcgttgc tgccgccagt ggctttcgcg
    15901 gtgggcgaat cttcccggca gtgtttgtcg gcgtggcatt agggttgatg cttcatgagc
    15961 acgttcccgc cgttccagcg gcaataaccg tttcctgcgc tatactcggc atcgtgctgg
    16021 tggtaacacg cgatggctgg ttaagtcttt ttatggcggc agtcgttgta cccaatacca
    16081 cattgctacc gctgctctgt atcgtcatgc tcccggcatg gctgttatta gcaggtaagc
    16141 cgatgatgat ggtcaatcgt ccgaagcaac agccacccca cgataacgtt tagcaaaaaa
    16201 tgctctcctt tatgatttaa gagttatggc gatgatacgg cgctttacct ccccgtaata
    16261 ttgcgttaac aggccgctga cggcttcgat cgtgaaggag aataacaatg ttcaggtcac
    16321 tgtttctggc ggccgccctg atggcattta ccccgcttgc ggcaaacgca ggtgaaatca
    16381 ccctactgcc atcaatcaaa ttacaaattg gcgatcgcga tcattacggt aattactggg
    16441 acggtggtca ctggcgcgac cgtgactact ggcaccgcaa ttatgaatgg cgtaaaaacc
    16501 gctggtggcg tcatgataat ggctaccatc gtggctggga taagcgtaaa gcgtatgaac
    16561 gtggctatcg tgaaggctgg cgcgatcgtg acgatcatcg tggaaaaggc cgcggacatg
    16621 gacaccgcca ttaaacacat tcgccaatgg agcacaatgc ctgatgcgac gctagcgcgt
    16681 cttatcaggc ctacaaacca gctctttaac gcgtagggcg ggtaaggcgt tttacgccgc
    16741 atccgccaat ggtgccaaat gcgacgctca ttcaactaca atcccagtgc cgttcctacc
    16801 agtaaccaga tattcagcgc cactaccagc accacaatca cccagcctgt ctgttttacg
    16861 cgtttactgt tcaccagatc gcccatcaac ttgctgtcac tggtgaaaat caacagtgga
    16921 accagcgcca gggcgatacc aaaacttaac agcacctgac tcataaccag aatccgtgtc
    16981 ggatctaatc ccatcagaat gacaataaat gacggcagca tggtgacagt acgacgcacc
    17041 cacagcggga tatgaaagcg aatgaagccc tgcatcacta cctgccccgc cagcgtcccc
    17101 accaccgttg aggacaaccc ggcagcaacc aggcttaatc caaagaccgt tgccgcagca
    17161 tggcttaaca gcggttgcag cgtcagataa gcctcatcaa gatcagcaac accagtatga
    17221 ccagaaaagt ggaacgccgc cgcagctgta gccatcatcg ccagattgac aaaaccggca
    17281 atcgtcatgg cgatagccac atcccatttg gtggcggaat aacgttgttg acgcgaacca
    17341 ccatgtaaat gctgagtgag cgaagagtgc aaataaatca catgcggcat aatcgtcgcc
    17401 cctaacacgc ccgcggccag gaagaccgcc tccgaagtag gtaaactcgg gattaccatt
    17461 cctttaccca gctgcgccag gttaggctgg gagaaaatca actcgacaat gtaagcagcg
    17521 gcaacaaaca acagtaaccc gccaatcact ttctccagcg gtttttgccc gcgacgttgc
    17581 agcattaaaa taaggaaagt cgcgattccc gtcagcaccg cgccctgcaa caacgaaaca
    17641 ccaagaatga gtttaaaacc gatcgccgca ccaataaatt ccgccaggtc ggttgccatc
    17701 gcaataattt ctgcctgaac ccaatagaac cacacgacgg gacgcgggta gtgatcgcga
    17761 atctgctccg ccagattttt accggtggca atccccagtt tagccgagag gatttgaatc
    17821 agcatcgcca tcaggttagc ccaaacgaca acccacaata actgatagcc gaagctggca
    17881 cccgcctgaa tattggtcgc aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac
    17941 gcaggtccca ttaatgcgag cctcatcttg cgcgccgccc gtccgctgct actctcaacg
    18001 cgatagttcg tcatcttgtg cctctaaaac atagcctttg ctatgtttca tgctacgtta
    18061 aatgagaatg attatcaaat tcatttaaat gggttgtgat gatttctctg atagaccagg
    18121 attatgacta cgaaaagata gatgactcga atgttatggt tatgtttaat attagcacat
    18181 ttacataacc ttccgcttcc atacacaaca tagcagaaat gtatgacaga tcactatttt
    18241 tgaagcttgt cacaggacgt cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt
    18301 tacatagaat gtgcacggaa atttaacctg cctcatattt ggagcaaata tggaccgcgt
    18361 ccttcatttt gtactggcac ttgccgttgt tgcgattctc gcactgctgg taagcagcga
    18421 ccgcaaaaaa attcgtatcc gttatgttat tcaactgctt gttatcgaag tgttactggc
    18481 gtggttcttc ctgaactccg acgttggtct gggcttcgtg aaaggcttct ccgaaatgtt
    18541 cgaaaaactg ctcggttttg ccaacgaagg gactaacttc gtctttggta gcatgaatga
    18601 tcaaggcctg gcattcttct tcctgaaagt gctgtgccca atcgtcttta tctctgcgct
    18661 gatcggtatt ctccagcata ttcgcgtatt gccggtaatt atccgcgcaa ttggtttcct
    18721 gctctccaaa gtcaacggca tgggcaaact ggaatccttt aacgccgtca gctccctgat
    18781 tctgggtcag tctgaaaact ttatcgccta taaagatatt ctcggcaaaa tctcccgcaa
    18841 tcgtatgtac accatggcag caacggcgat gtccaccgtg tcgatgtcca tcgttggtgc
    18901 atacatgacc atgctggaac cgaaatacgt cgttgcagcg ctggtactga acatgttcag
    18961 cacctttatc gtgctgtcgc tgatcaaccc ttaccgtgtt gatgccagtg aagaaaatat
    19021 tcagatgtcc aacctgcacg aaggtcagag cttcttcgaa atgctgggtg aatacattct
    19081 ggcaggtttc aaggttgcca ttatcgttgc cgcgatgctg atcggcttta tcgccctgat
    19141 cgccgcgctg aacgcactgt ttgccaccgt gactggctgg tttggctaca gcatctcctt
    19201 ccagggcatc ctgggctaca tcttctatcc gattgcatgg gtgatgggtg ttccttccag
    19261 tgaagcactg caagtgggca gtatcatggc gaccaaactg gtttccaacg agttcgttgc
    19321 gatgatggat ctgcagaaaa ttgcttccac gctctctccg cgtgcggaag gcatcatctc
    19381 tgtgttcctg gtttccttcg ctaacttctc ttcaatcggg attatcgcgg gtgcggttaa
    19441 aggcctgaat gaagagcaag gtaacgtggt ttctcgcttc ggtctgaaac tggtttacgg
    19501 ctctaccctg gtgagtgtgc tgtctgcgtc aatcgcagca ctggtgctgt aagaccatac
    19561 ataaaaaagc cggggataat tccccggctt ttttacgcct gttaatcagc taatggctgc
    19621 ggacgaccaa ttaaatatcc ttgcagatat tgcaccccaa gtttatgcaa tagcgcctgc
    19681 tgctgtggcg tctcgacaaa ctccgcgacc acactcaatg acttcgcttt cgccagatcg
    19741 gtaattgatc tcacaatcat cgcatccagc gtgttcgtga cgatatcttt cacaaagacg
    19801 ccatcaattt tgatgatatc agcctgcaaa cgctttaacc gttcgtagtt ggcatatccg
    19861 gtgccaaagt catcaatcgc aatccggaaa ccaaacttat gcagctgctc gatgttgtac
    19921 atgctgcttt ctgcgttaga aaacgcctgc tcctcggtga tctcaagaat gaccgcctgc
    19981 ggggagatgt aataacgctt aaacagacga ataatccgcc cggcaatatt cttttgcagc
    20041 agcgtgagcg gcattaaatt gactgaaaag cgtggacctt ttttgtcgca agggtgtgtt
    20101 gccagccact tcaacaagga ttccagcact tgcaaatcaa aacgcgcgct aaggttaaac
    20161 tgagcaataa ggggcagaaa cttatccggg gtcataatgc cgccgtcata cttcagtcgc
    20221 gcgaggatct catcataacc ttcaccctct ttgttgcgaa ttggctgggc gtagagcagc
    20281 aaatcaccct gatctaacgc cgtgcgaatg gtattcagca atagcacctg tttagtggtt
    20341 tgcccggaaa ccatctcctc tctgctatcc agcgccagca catgatgatg tgcgcaggat
    20401 tgctccgcca gccagcttaa ctgccccaac aagggttgca gggtttcctg atttccatca
    20461 aaacgtcccc aggcagcacc atagcccata tccagcccgg tattgttcca gtgaatttgc
    20521 cgactattca ggatgttaac catatgctgg agtcgccctt ccgtttccgg cccgctcagc
    20581 actaacagca gttcactacc cggcaattga tacaactttt cgttttcctg catcaacggc
    20641 agcagcgtac ggtaaattga gcggatacag tgaacgcgca tcattaagcc gtaatgacga
    20701 ctcataaact caagattatc aatgcgcagg cagcaaaaac tcttgcccgc ctcttgctcc
    20761 ggcgcttgct ccaacgcacg aaagttgggt agaagcgtta acggatcggt cagcgcctgc
    20821 aaatgccagc ggcggttaag ccattcacta cgatgataaa tacgcaccat atagagcagg
    20881 caaacactaa aggaaatcag tactgcgaga ataaatgcca gcgaatattc ggttagtacc
    20941 ccttgcaaaa agttctggtt gtaattcaga aggcaaagcg ttgaaacagc ccaagtgaga
    21001 tttaaaaacg gatagcgaag cttaccgaca ccgagggtaa agatgataaa gaaaacaggt
    21061 accaggtaac cggcaataaa gtcattttca tacggtgtgc acatcagaag tagcatcacg
    21121 ctaagagctg ccagccagct taaggtaaat gcgcgtttct ctttgctcaa cgacggagcg
    21181 atatccctac gccacaatat ctgtgcaaag tggggactta caatcatgcg ggtgagatag
    21241 taaaaaagca tgttgtaaat cagcactgcg gtgaaaaggc ttagcaaatc aacgaccgtg
    21301 aaaatggcat ccgcatcgcc gaaaaaggta gatatcttta gcggaaaatc aaagaaattt
    21361 cccacaagat acatgctgca tttgatgcca atcggcgcca ccaaaccaag ccagaacaga
    21421 cgttgccaga cgtatcggct ggtcaatcca taacgccaac gcgtccccaa ttgccagcgt
    21481 aatattgcac aggcacacag gactgcaaac gcctggctgc cgatcatgac ggcggtctgt
    21541 aaaaaagaga gatgcaggtt ccacagattg gtgcaaaaca tcccgcataa aatgggaacg
    21601 acaccacgcc agccaaagat aaacaagatt gacagcatta cgcacagtgg catccacgcc
    21661 agaaagatat aattggaatg gataacagca agtggcgaaa ttaaacggga tagctgaata
    21721 agtaccacgg tgagcgtaaa cgctagtgtg aatatcttga tattttttat caggttatgc
    21781 tccacaaaca tgagtacact actctcactg atcggggttt tcaggcacac aaataataag
    21841 tttattcgca tcgcgaagca aacaggggcc agcctgttgc tgttttttca gtcagtgatg
    21901 cattcaccac acggcactgc caggatgtag actgtattac aaaagcggca aaaggcagag
    21961 acaaaaaacc cccgctttgc agcgagggtt tgaaatttgg tggagctaag cgggatcgaa
    22021 ccgctgacct cttgcatgcc atgcaagcgc tctcccagct gagctatagc cccacgagtc
    22081 gtttacgtac caaatttgct gggtgcaaaa tttggtggag ctaagcggga tcgaaccgct
    22141 gacctcttgc atgccatgca agcgctctcc cagctgagct atagccccat cacgtaaagc
    22201 ttgtcgagtt gacgggcggc atcatatgaa ttccgcccga atgtgtcaac ggcaaattgc
    22261 cacgtgtagt ttcaatcgct gaaaaatcag gcaaatgaac aattttggaa aaccgctcgc
    22321 attcagtagt tattcatgtc acggtttcct gtaaagtggt gttataaaat gaactactaa
    22381 tagacccaca tacattcagg gaattgttat gttcaaggag aggatgacgc cagatgaact
    22441 tgccagactg accggctata gccgccagac cattaataaa tgggtacgca aggaaggctg
    22501 gacaacgtca ccaaaaccag gcgtccaggg tggcaaagct cgactggttc acgtcaatga
    22561 acaggttcgt gaatatattc gcaatgctga acgtccagaa ggtcagggag aagcgccagc
    22621 cctttctggt gatgcgccgc ttgaagtgtt gctggtgaca ctggcgaaag agatgacgcc
    22681 agttgaacaa aaacagttta catccttgct tctgcgggaa gggattatcg gattattaca
    22741 acgcttaggg attcgcgata gcaaataata tgaaaagatt acgcaataaa atgaccaccg
    22801 aagaactggc tgaatgcctc ggtgtggcta aacaaactgt taaccgttgg atcagagaaa
    22861 aaggctggaa aacggaaaaa tttcccggcg tgaaaggcgg tcgtgccaga cttattctgg
    22921 tcgatacgca agtttgcgag tttattcaga acacgccagc cttccataac acgccgatgc
    22981 ttctggaagc cgaagaaccc cttgccgaat acgcaccggg gattcgcact cctgcttatc
    23041 ggcaaatcat cagcgcaata gacaacatga ctcacagtga gcaggaaaaa gttgcgcaat
    23101 ttttgtcacg cgaaggaatt cgtaattttc ttgctcgtct cgacatagat gaatcagctt
    23161 gacatcctcc acgccctgaa tgacgaggat ccctgctacg ttcaggctgt cgcctgaatc
    23221 atttcggtgg gttcctgctt caacgggcgg cctgactgca ccatctctcc acaggcaaga
    23281 acggcgtgcc ccgccgctaa aatgttacga gcgccgttta catcggcgtt cgctgtatat
    23341 ccacatacct ggcatctgaa tttactttgt gacaggcggt tttctttcgc tgtatgacca
    23401 cagcacgcgc aacgctggct tgtgtatgct ggcggaaccg caagcacctg accgccacgc
    23461 cagagcgctt tgtactccag ctggcggcgc atctcatacc agccctgctc ctgtatcgga
    23521 cggtttaaac ctgattttgc ccggacatgg cgccccggct ggctgaccgt acccgctgct
    23581 gactttgaca tgttgctgac cttcaaattc tcaatgactg tcatggcgtg gtttttgctg
    23641 atggtcgtgg tgactttgtg aaggtagtcc ctgcggatac tggcgatacg ggaatgcaga
    23701 cactgtattt tgcgtttctg cttctgccag ttgttgctga atttgaccct gcggcttaac
    23761 tgtcgctgaa gtcttgccag cgtcttctgg tttttctgaa aactgtttac aggctcaaag
    23821 actgtgccat ctgacagcgt ggcgagttta gccacgccag catccagccc gaccattgat
    23881 gctgaagggt gaaccggagt tgatacttca ctttctgtct gaatactgat gtgccactta
    23941 ccgcaggact ggctgaccgt gacatttttc acaacacccg tgacctgacg gctattccgg
    24001 tagcgcatcc agcccagttt cggcagaaaa atacggctgt tttcctgatc gagcttaacg
    24061 ccctgcgggt agcggaatga atcattctgt ccccgctttt tgaatcgggg aaaagcagcc
    24121 cgcttctgga agaagttttt gtaagcccgc tcaaggtctt tcagtgactg ttgcaatggc
    24181 tgtgacgggg catttttaag ccattgcgtt tcagtggcat ttttccactc aaccagccag
    24241 gaagccattt tcccgtaagg gatgtattta ttaccggcct catgattctc attctgaagc
    24301 gccagagcac gattgaaaac gaaacgacat gcgcctgcga agcgcctcat ttcacgctcc
    24361 tgttgaccac cgggtcttaa ctggaattta aatgcctgta gtcgtttcac aggccaaatg
    24421 atactttggt ctgtgaaaaa agaaaccgat atccggcgtg gaaggcattg tgttttcctg
    24481 atgcatgttc acctggtctt tgtcccaaat accgacgcca gatttttgac catgacgcga
    24541 cagaaaagct acgcacttac ttttcaaatg tatgtgctga ttttgaagct gaactggttg
    24601 aaatggatgg agagcgagag cacgtccatc tgttaattaa ctatcctccc aaactggcga
    24661 tatccagtct ggtaaacagc ctcaaaggtg tatcgggtag gtgactgcga cgagatcggc
    24721 cagatattgc agtcaggtat tactacaaag gcattttgtg gagtcctggc tattttgcca
    24781 gtagctgcgg aggtgcgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
    24841 ctggtcaggt ggaaaaccgc gccttatatc cccgccctga aggacggggt tttacggcgc
    24901 accggataaa taaaaaacgg caggggtatc tctcctgccg tttatctttt atgcgctaat
    24961 tactgctgat tttcgcgttc agcaataaaa gccagcgctt tgttgatacg ctcgatactg
    25021 cgggtcttac cgattgcgtg aacggtaaca tccagcgccg gagactgccc cgcaccggtt
    25081 acggcgacac gcagcggcat accaacttta cccataccca cttccagctc atccgccgtc
    25141 gcctgaatag cgtgatgaac gttttcagcg gtccagtcag taatcgcagt cagtttgtca
    25201 cgaaccactt ccagcggctg gcgcgctacc ggacgcagat gttttttcgc ggcgtcggca
    25261 tcgaactcag caaaatcttc gtagaaataa cggcagctct gtgccatctc tttcagcgtc
    25321 ttgcagcgtt cgcccaacag tttcaccaga tcagccagct gcgggccgtt acgggtatcg
    25381 atattttcct gctcaatgtg ccactgtaag tgagtagcaa catactccgg cggcaacgcg
    25441 ttaatgtagt gatggttcag ccacagcagc ttgtcggtgt tgaacgcact ggcagatttg
    25501 ctgacggcat tcagagtgaa gtatttaatc atctcttcac gagtgaagat ttcctgatcg
    25561 ccgtgggacc agcccagacg caccagatag ttcagcagtg cttcaggcag ataaccatca
    25621 tcacgatact gcattacgct gaccgccccg tgacgtttgg acagtttttt accgtcatcg
    25681 ccgttgatca tagaaacgtg cgcgtaaacc ggtaccggcg ctttcagagc cttaagaatg
    25741 ttaatctggc gcggtgtgtt gttgatatgg tcttcgccac ggataacgtg ggtgatttcc
    25801 atatcccagt catcgacaac cacacagaag ttataggttg gggaaccatc ggtacggcgg
    25861 ataatcagat catccagctc ctggttgctg aactcgatcg gaccacggat ctgatcgtca
    25921 aaaacaacag aaccttcctg cgggttagca aaacgtacga cacacggctc atcatcagcg
    25981 tgatgctcat gactgtggcg gcagcgaccg tcataacgcg gcttctcacc tttcgccatt
    26041 tgctcttcgc gcagcgcttc caggcgctct ttagagcaat agcatttata agctgtgccc
    26101 tcttccagca tctgatcgat caccgcgttg tagcgatcaa aacgtttggt ctggtagtac
    26161 ggaccttcat cccactccag gttcagccag ttcatgccat ccataatggc ttcgatagct
    26221 tccggcgtgg aacgctcaag atcggtgtct tcaatacgca gcacgaactc accgccgtgg
    26281 ttacgtgcaa aaagccagga gtaaagagca gtacgcgcgc cgccaacgtg cagatagcct
    26341 gttgggcttg gcgcgaagcg agttttgatt ttcatgaaat ggccttacgt ttagaaagat
    26401 gccgacaacc ggcaaatcct ggaaaaattg agtgggcgat attctatcac tcccgcctga
    26461 ttcctcaatg tagttcgggt tgcaacctta cgcattattg ttttcgttga caaattgcgc
    26521 aacacggtct gttttgcgat catcttgctt aaatttacga cgaacgaaca atttctttag
    26581 aaaatgcgtt gactcatttt gaactctccc tataatgcga ctccacacag cgggggtgat
    26641 tagctcagct gggagagcac ctcccttaca aggagggggt cggcggttcg atcccgtcat
    26701 cacccaccaa ctactttatg tagtctccgc cgtgtagcaa gaaattgaga agtgggtgat
    26761 tagctcagct gggagagcac ctcccttaca aggagggggt cggcggttcg atcccgtcat
    26821 cacccaccac tttctcgcca gctagatttc ttgtaacaat gtgaagtacc gaagtgggtg
    26881 attagctcag ctgggagagc acctccctta caaggagggg gtcggcggtt cgatcccgtc
    26941 atcacccacc acttcgggtc gttagctcag ttggtagagc agttgacttt taatcaattg
    27001 gtcgcaggtt cgaatcctgc acgacccacc aatgtaaaaa agcgccctaa aggcgctttt
    27061 ttgctatctg caatttgcga aattgcctga tgcgcttcgc ttatcagacc aactatgtcc
    27121 ggcagttccc gtgcctctca tctactgcgc caacgccgcc agcagtttaa ccagcgcggg
    27181 cgtcacctgc tgcgtttcat aaacaatata taagtctgcc gggatgcgtt ctttgagcgg
    27241 acggaatatg acaccgggcc agttcatctg cgcgtagctg tccgctatca atgtgatacc
    27301 aatacccata ctgaccatgg cgagtaccgt ttgcggttca ttaacttcgc gaataacaac
    27361 cggtgaaaat cccacctgct ggcaaactcg ctgcaaaaaa tcccagtcag tgtacacggg
    27421 cggcattgta acgaaatact cgtcacgtag cgcttccagc gggacggtgg aaaatgatga
    27481 gagatgatgc tcttcaggca tcgccaccag aaacgccgat tcatgcaacc gtaagctggt
    27541 aaaaccagtc ggtggttctg tcgccattcg ccagatcccg gcatcaagtt cgcggcgttc
    27601 cagcaaggcc atttgcatcg caggcatctt ttcgcgaaac agaacttcaa cgttaggatt
    27661 ttctctaagg aatcgccgca taaccgggcg catccgtccc cacattgccg ttcccacaac
    27721 cccgagttca atccgccctg cttctccccg acctatttgt tcaacccgag ccaatacatt
    27781 atttgcattc accagcaatc gacgcgattc ttccatcaag attttgcccg cgtgtgtcag
    27841 tacgacgctg cgcgaatggc gaataaaaag ctgcgtgccg agttgatttt ccagctcttt
    27901 aatatgaata ctgagcggag gctgagacat atttaaacgc gctgctgcgc gaccaaaatg
    27961 caactcttcc gctacggcaa gaaaataacg gagcaactta agatctgttc tatatacgcg
    28021 ttccataatt aagtgctcca tataaaaaat atatcgtatt aaattatcat ggcataaagt
    28081 atttcaccac cagatatccg acatacataa acacgatgga taatataccc ggcaatacat
    28141 ttggaacagg aataggtaat cgcacgattg taaacaaagc accaatgcca aaaccaacgc
    28201 cagaagaaaa taaaatatct ttcactaatt aacctttatc ataaaagcag ccccaaggaa
    28261 cagagctgcg aatcctttta atgtgtaact gaacgatgcg ttatcttttc agggtcatgg
    28321 ttatatttaa acccaaagaa aaatatcact gcgaggaaaa gagcataccc tgcaaacatc
    28381 agccaaatgg tatgccaatc ttttacgcca tccaccgaga agtaatccac tgccataccg
    28441 ctcagaatcg agccaaccca tgcgccgacg ccatttacca tcgtcataaa aaggccctgg
    28501 gcgctggcac gaatgctgga atcaacttcc tgttcgacaa agaccgaacc agaaatattg
    28561 aagaaatcga atgcacaacc gtaaacaatc atcgacagca acagcaaaat aaacccggtt
    28621 ggtgatggat cgccataggc aaagaagcca aagcgcagcg tccacgccac catactcatc
    28681 agcatgacgg ttttaatacc gaaacgcttc aaaaagaacg gtatcgtcag aatgaaaccg
    28741 acttcagcca tctgtgaaac tgacagtaaa atggacgggt atttcaccac gaagctatca
    28801 gcaaactcag gattacgggc gaaatcatgc agaaatgggt tgccaaaaac gttggtaatt
    28861 tgcagcaccg cgcccagcat catggcgaag aggaaaaaga tagccatgcg tggattttta
    28921 aacagcacga aggcatccag acccagcttg ctggcaagcg atgtagtcgc ttttttctcc
    28981 gcaaccggaa tcttcggcaa agtcagcgca taagccgaca gcagcaatga cgcaccggac
    29041 gcgatataca gttgcagact actcaattcc agatgcagca ggcttactgc ccacatggcg
    29101 acaatgaacc ccaccgtacc aaaaacgcga atgggcggga aagcggtcac cgggtcaagc
    29161 cctgcctggg caagacagga ataagagacg ctgttcgata acgcaatagt cggcataaac
    29221 gccatcgcat tgactaacat cacccaaaac atcatatccg gatcagttac ggatgccgca
    29281 taaaaaagta cgcccgcaca caccaagtga cacagcatgt atgcacgttc tgcacgcagc
    29341 catttgtctg cgatgatccc cattataccc ggcataataa tcgcggcgat ccctttggaa
    29401 ctgtaaacca tgccaacatt agcgccggtg aaatgaaggg tattaatcat gtaagaaccg
    29461 agggtaacca gccagctccc ccagataaaa tattgcaaaa aggacattac ctttaagcgc
    29521 aaagcgatgc tcataatcgt ttccttgtcg catcatatgg cctcactcag tgaggccgtt
    29581 tattgttgtt ttactcaggc aattttgcgc agaaagccgc aaataagatt aataaagttt
    29641 tgctttgaga gttccgctgc tgccagcgtt tgggcatgag aaagcttcac gtcactcaga
    29701 ccttccgcca tgttggtaat cgcagagacc gcaacgactt taagttcgca atggcgagct
    29761 gaaataacct caggcaccac agacatacca acaacatccc caccaataat ttgcatcatg
    29821 cgaatttccg ccgcagtctc gaaattcggc cccggatacg agacgaacac gccctccgtc
    29881 agagggaacc cctcttcttt cgccactttt tgtaacagtg cgcggtattc cgcatcgtag
    29941 gcattcgcca gcgagaagaa gcgctcacca aaacgttcat cattaagacc caccatcggc
    30001 gttcccggca tggtgttgat gtgatctttc aatgcgacca gactgccggc ccccacttca
    30061 ggacgcagtg agccagccgc attggtgcag aacagcaact cgcagcccag taacttaaag
    30121 gtacggattg catccgtcat gatggtcatc ccacgacctt cgtagaaatg tccgcgacct
    30181 ttcatgcacg ccactggcac cccctggaga taccccagca ccagctcacc cgcatgaccg
    30241 tgtacggtac ttaccgggaa cccaggcagc ttttcgtagg aaattgcgac cgcgttctca
    30301 atctgatcgg ccagcgcgcc cagcccggaa cctaaaataa aggccactcg tggcgtgaaa
    30361 tcaggtttat aagtcttgat aatatctacg caatacagtg ggttatgaga aaactgagcc
    30421 tgatacatat atatcctttt tttgtagggt ggaatctaac gcctttttat aacgagattg
    30481 ccctacctca taccaataca cttttttcat gtatccgata gttattcgat atcgatatat
    30541 ttatattttt tcactattaa taggctgaaa aaactgtatt ggtactctcc tttcccttgc
    30601 attaacaata tgccgctttt aatcaacctt ttattaagca atcaactaat aacgatcggc
    30661 atagggaatt atgctatgaa aaaacatctt tttactctga cactctcttc tgtattggcg
    30721 ataccagcag tatcccatgc agaatttaaa ggcggttttg ctgatatagg tattcattat
    30781 cttgactgga ctagcagaac cactgagaaa tcgtcgacca aatcacacaa agatgatttt
    30841 ggatatctgg aactggaagg cggcgctaac tttagttggg gagaaatgta cgggttcttc
    30901 gactgggaaa acttttataa tgatcgtcat gataaaccgg gtagcgagca gcgttataca
    30961 tttaaaaata ccaaccgtat ttacctgggt gacaccggat ttaatctcta cttgcatgcg
    31021 tatggcacct acggttctgc gaatcgtgtg aatttccatg acgatatgtt cctttacggg
    31081 atcggctaca atttcaccgg cagcggttgg tggttcaaac cgttctttgc aaaacgctat
    31141 acagaccaaa cctattatac cggcgataac ggttatgtcg ccggttgggt tgcaggttac
    31201 agctttatgc tgggcagtga gaaattcacc ctcaccaact ggaacgagta cgagtttgat
    31261 cgtgatgcca cctacgcggc aggtaatggc ggcaaagaag gtctgaacgg tgccgtcgca
    31321 ctttggtgga atgcaacttc acacatcacc acagggattc agtatcgtta tgcggatgac
    31381 aaactgggcg aagattttta ccaggatgcg ataatctact ccatcaaatt taatttctga
    31441 ttgattactg ccggagctcc cggcagtatg aattcactct atatctgata caacggatcc
    31501 ccttcccgcc cagccattag cgcattgccc tggtctcgta ctacgtccca taacgcttca
    31561 gcggcggtgg aaagtgaacg gttcttcctg cgcaccagca tcaactggcg ttcaaccact
    31621 ggcgtaatgc gctttaccac cagtgggcta ccttcaggca atggtagcgc cagtgcggga
    31681 agaatactga tgccgatgcc tgcggctacc atcgggaaca gcgtcgccgg atgaccaatc
    31741 tcctgcacaa tattcgcctg aataccgttg cgcgccagcg ctgcgtcaat cagcggtcgg
    31801 ctgcctgacg cgtaatcctg caacaccaat ttcgctccct gtaatgcctg ccagggtaca
    31861 taatcttcca cggccaaagc gctatcgcga tggcaaagca gaaagaaagg ctcggaaaga
    31921 atcgcttcac attgcagatc gcccactggg cctggatcga tgacgatgcc aaaatcgaca
    31981 tctccctggc gaatactttc catcacccac tgctgcggac gatcgtgcag gacaaactga
    32041 atatctggat agcagcgatg gctttcagca atgcattgcg gaataagatg cgcggaaatg
    32101 gtctggctgg cagcgacccg aacttttccg ctcagttgtt gccccatacg cccggtatcg
    32161 cgcaacgtgc tgttaagttc atccagcagt cgctcaagac gcaaagccaa ctgctgccct
    32221 gcatctgtaa gcaccacttc acgcgtggtt ctgtccagca ggcgaacacc agtatgattt
    32281 tccagctcct tcacactgtg actcactgcc gactggctca ggccgatacg ctctcctgca
    32341 cgactaaaac ttttctcctg cgctactgtg acgaaaacct ttaattgctt taaagaataa
    32401 ttcatctgtt tttttcatga atagatgcaa taaatcaatt ttatttctca aactggaaga
    32461 agcacaatag aaccatcgat catctggagt ctttatgaaa ctttttcgta tcctcgatcc
    32521 tttcacctta accctgatca cggtggtgtt gctggcctct ttctttccgg ccagaggcga
    32581 tttcgtcccc ttctttgaaa atctgaccac tgcagcgatt gctctgctgt tctttatgca
    32641 cggcgcgaag ttgtcgcgtg aggcgattat tgctggcggt ggtcactggc gactgcattt
    32701 gtgggtaatg tgcagcacct tcgtgctgtt tccgattctg ggcgtgctgt ttgcctggtg
    32761 gaaaccggta aatgtcgacc cgatgctcta ctctggtttt ctctacttgt gcattctccc
    32821 cgctaccgtg cagtctgcaa tcgccttcac gtcaatggcg ggcggtaacg tcgcggcggc
    32881 ggtttgttct gcgtcagcat ccagcctgct ggggattttc ctttcaccat tgctggttgg
    32941 tctggtgatg aatgttcacg gtgcaggggg cagccttgag caggtcggta aaattatgct
    33001 gcaactgttg ctgccatttg tgttggggca tctttcccgg ccgtggattg gtgactgggt
    33061 gtcgcgtaat aaaaaatgga ttgcgaaaac tgaccagacg tccattctgt tggtggttta
    33121 tacggcattc agcgaagccg tcgttaacgg tatctggcac aaagttggct ggggatcatt
    33181 gctgtttatc gtggtggtca gttgcgttct tctggctatc gtgattgtag ttaacgtctt
    33241 tatggcacgc cgactgggct tcaataaggc agatgaaatt actatcgtct tttgtggttc
    33301 gaaaaagagt ctggcaaatg gcatcccgat ggcaaacatt ctgttcccca catcggtgat
    33361 cggtatgatg gtgctgcccc tgatgatttt ccatcagatc caattgatgg tctgtgcggt
    33421 gctggcgcgt cgatacaaac gccagaccga acagttacag gcacagcagg aaagcagcgc
    33481 cgataaagct taaagcggac gcttcagggg ctggaccagt tgcgtcagcc cctcggtttt
    33541 aatcagcagc gtgatttgca ttagctcacc gagtttccct gccgggaact catctttgcg
    33601 ggcaaaccac agcagatact cctccggcag gtcgattaag cgacgccctt tgtacttgcc
    33661 aaacggcatt atcgtattgg ctatttcaat cagctgctct ttttccatct cagctaccca
    33721 gcaaacgcag catttccgtt tcgtcgatga cttcaatgcc cagttcctgc gccttcgcca
    33781 gtttagatcc tgcagcttca cccgctatca ccagatcggt tttcttcgac acgctgcccg
    33841 cgactttcgc ccccagttcg accagtcgag ctttagcgtc atcacgcgac atctggctta
    33901 agctgcccgt aagcaccacg gttttaccag caaacggact gtcaatctct tccgcattga
    33961 taacgatcgg ctcaggccag tgaacacctt ccgccaacag ctcgctgatg acattgcggt
    34021 tgctttcttc ggcaaagaag ttgtgaacgt gggaggcaac gacaattcca acatcaggca
    34081 ccttttgcag ctcttcaatc gacgcggctt ccagcgcttc cagcgtgccg aaatatgccg
    34141 ccagacctgc tgcggtagcc tcgccgactt cacggatgcc aagtgcatag aggaagcggg
    34201 caaaggtggt ttctttcgct ttttccagcg cgttaaccac gttttgtgcc gatttcggcc
    34261 ccatacgctc cagtccggtc agtttgactg cggtgagttt gaacagatcc gccggagtgt
    34321 ggacatattc tttttcaacc agttgatcga tgattttgtc gcccattccg tcaacatcca
    34381 tcgcacggcg ggaaacaaag tgtttcagag actctttacg ctgcgcaccg caaatcaggc
    34441 caccggtaca gcgggcaacc gcttcacctt ccacacgctc gacgtcagaa ccacataccg
    34501 gacaatgcgt cgggaataca acctcacggg tatcttccgg gcgttcagaa agcacgacgt
    34561 taaccacctg cgggatcacg tcgccagcgc ggcgaataac cactttatcg ccaatgcgta
    34621 aaccaagacg ttcgatttcg tcagcattat gcagtgtagc gttactcacc aacacgccag
    34681 caacatggac aggttccaga cgcgcaacag gcgtaatcgc gccagtacgc ccaacctgaa
    34741 actcgacgtc acgcacaaag gtcatctgtt cctgcgccgg gaatttaaac gctaccgccc
    34801 agcgcggggc acgcgcgaca aagccaagct gctcctgctg tgccagtgag ttgaccttaa
    34861 tcaccacgcc gtcgatatca aagcccagcg tcggacggtc ttcttctact ttgtggtaga
    34921 acgccagcac ttcttccgcc gattcacaaa gtgttacccg atcactgaca ggtaatcccc
    34981 acgctttaaa ttgcattaag cgtcccaaat gggtatccgg caattcaccg ccttccagaa
    35041 caccaacgcc gtagcagaag aaagtgagcg gtcgcttcgc tgtaatacgc ggatcaagct
    35101 gacgcagtga accagctgcc gcattacgtg ggttagcaaa cactttcccg cccgtgcgtc
    35161 gcgcatcttc gttaatcttt tcgaaccccg cctgcggcag gaacacttca ccacgcactt
    35221 ccagacgcgc cgggatattc tcaccgtgta gcttcagcgg aatggcgcga atagtacgca
    35281 cattagacgt gatatcttcc ccggtggtgc catcgccacg agtcgcggca ctgactaaaa
    35341 cgccattttc atacagaata ctgacggcaa gaccatccag cttcagctca cagcaccagg
    35401 tgactttctc gttgcttttc agacggtcct gcacacgttt gttgaaagca agaaagcttt
    35461 cttcatcaaa aacgttatcc agcgacagca ttggcacttc atggcggatc tggctgaaag
    35521 ccgccagcgg cgctgcacct acacgttggg taggcgaatc aggcgtaatc agttctggat
    35581 gtttggtttc cagctcgcgc agttcgcgca tcagcctgtc gtattcagcg tcgggaattt
    35641 ccggcgcatc catcacatga taaagatatt catgatggcg aagcgtcgtt cgcagttctg
    35701 ttagttgttg ttcgattgat tccatatcgc accatcaatg ctaaaaaccc ccgacaagcg
    35761 ggggttcgaa gaggagttaa tttgccttaa gtgtatcagg cgttggcgtc tttgacttcg
    35821 cggatgatgt cctggtactc gcgcaatttc tgcggagtca tcatacggcg ctgatcgtca
    35881 agcactacac cgcccacttc atcggcaata tgctgcgcag attgcagcat cagcttaaag
    35941 ttctgcagct cgtcaccgta agacggtacc tgcataaaga tagtgacacc cggagtagtg
    36001 aaatccttca tttcaggatc aaaggttccc ggtttcacca tattcgccag gctgaataac
    36061 gccgggccgc tgccatccgg gctaagatga cgatggtaaa tgttcatatc gccaaaaatg
    36121 aagcccgctt gttgaatgct gttaagaagc agttcaccgt ttagctcgct accgtgatgc
    36181 gccgcgacgt tcatgataat caccgcttct ttgcgcttcg gtttatccat aactggagcc
    36241 ggttccgcta caggctcagg ctgtggtgcc gctacaggct ctgcaggctg gaaagcctgt
    36301 tgtgctggtt gcggtgctga atgcactggc tgcggcgctg acgcgacctg tggcgaaaca
    36361 ggctgctgca acggctgttc aggctgcggc tgataggcag gctgctgcac aggttgctgc
    36421 accggctgcg ttggacgcgg agcatgttgc ggcggtacct gcgcttcagg cggctgctgg
    36481 accggttgac gcggctgcgc agacgcataa ggcggttggt actggtgttg cggcgacgga
    36541 cgagcagcct catgctcctg ggcgttagcc ggggcatgat tcacgcggtg aacacgaacc
    36601 tcaccaacgc cctcatcatc ttcgacatcc tcgtcataag aatcgtcgtc acgttttgac
    36661 ttcattcgtt ttaatggccg atcgcggaac atagaagatc gttctttacg gctggtccag
    36721 aaaccatgta ccagtaaagc gattatggcg atcgcgccaa caatgattaa tatcagacgc
    36781 aaatcctgca tcattatatt ctctgttgtt ctaacacctt gccaccacgg caaacattta
    36841 ctcactaaga gtatttgccg attacctcaa gtgcaagtgc actattaact ttcacagcac
    36901 aaagatagat gaaatcgtgc tttttgctgt tttttcgaac atatcctaac tgtccattgc
    36961 gcaattaccc ggtaaaatac gcagaatttt cctgggattg gtcaaaagga gctcatcctg
    37021 attatggttt catcattcac atctgcccca cgcagcggtt tttactattt tgcgcaaggc
    37081 tggaagctcg tctcgcaacc tgggattcgg cgtttcgtta ttttaccgct gctggtcaat
    37141 attttgttga tggggggcgc attctggtgg ctctttacgc aactcgatgt ctggatcccg
    37201 actctcatga gttatgttcc ggactggtta caatggctga gctatctgtt gtggcccctg
    37261 gctgtcatct ctgtgctgtt agtgtttggc tatttcttct caacgattgc taactggatt
    37321 gctgctccgt tcaacggttt attggctgaa caactggaag caagactgac tggcgctaca
    37381 ccgccagata ccggaatttt cggtatcatg aaagatgtgc cgcgaatcat gaaacgcgaa
    37441 tggcaaaaat ttgcctggta tctgccgcgc gcaattgtat tgctaattct ttacttcatc
    37501 cctggcattg ggcaaaccgt cgcgccggta ctgtggttcc tgtttagcgc ctggatgtta
    37561 gccatccagt attgcgatta ccccttcgat aaccacaaag tgccgtttaa agagatgcgc
    37621 accgccctgc gcacacgcaa aatcaccaat atgcagtttg gtgctttaac cagcctgttt
    37681 acgatgatcc cgttgcttaa tctgttcatc atgcccgttg ccgtttgtgg tgcgacggcg
    37741 atgtgggtcg attgctatcg cgataaacac gcgatgtggc ggtaacaatc taccggttat
    37801 tttgtaaacc gcttgtgtga aacaggggtg gcttatgccg ccccttattc catcttgcat
    37861 gtcattattt cccttctgca tatagatatg ctaaatcctt acttccgcat attctctgag
    37921 cgggtatgct acctgttgta tcccaatttc atacagttaa ggacagacca tgagtaagat
    37981 ttttgaagat aactcgctga ctatcggtca cacgccgctg gttcgcctga atcgtatcgg
    38041 taacggacgc attctggcga aggtggaatc tcgtaacccc agcttcagcg ttaagtgccg
    38101 tatcggtgcc aacatgattt gggatgccga aaagcgcggc gtgctgaaac caggcgttga
    38161 actggttgaa ccaaccagcg gtaataccgg gattgcactg gcctatgtag ctgccgctcg
    38221 cggttacaaa cttaccctga ccatgccaga aaccatgagt attgaacgcc gcaagctgct
    38281 gaaagcgtta ggtgcaaacc tggtgctgac cgaaggtgct aaaggcatga aaggcgcaat
    38341 ccaaaaagca gaagaaattg tcgccagcaa tccagagaaa tacctgctgc tgcaacaatt
    38401 cagcaatccg gcaaaccctg aaattcacga aaaaaccacc ggtccggaga tctgggaaga
    38461 taccgacggt caggttgatg tatttattgc tggtgttggg actggcggta cgttgactgg
    38521 cgtcagccgc tacattaaag gcaccaaagg caagaccgat cttatctctg tcgccgttga
    38581 gccaaccgat tctccagtta tcgcccaggc gctggcaggt gaagagatta aacctggtcc
    38641 gcataaaatt cagggtattg gcgctggttt tatcccggct aaccttgatc tcaagctggt
    38701 cgacaaagtc attggcatca ccaatgaaga agccatttct accgcacgtc gtctgatgga
    38761 agaagaaggc attcttgctg gtatctcttc tggagcagct gttgctgcgg cgttgaaact
    38821 acaagaagat gaaagcttta ccaataagaa tattgtggtt atcctgccgt catcgggtga
    38881 gcgttattta agcactgcat tgtttgccga tcttttcact gagaaagaac tgcaacagta
    38941 atgccagctt gtaaaaaatg cgtaaaaaag caccttttta ggtgcttttt tgtggcctgc
    39001 ttcaaacttt cgcccctcct ggcattgatt cagcctgtcg gaactggtat ttaaccagac
    39061 taattatttt gatgcgcgaa attaatcgtt acaggaaaag ccaaagctga atcgatttta
    39121 tgatttggtt caattcttcc tttagcggca taatgtttaa tgacgtacga aacgtcagcg
    39181 gtcaacaccc gccagcaatg gactgtatta cgctcttcgt gcgtcgcgtc tgttaaaaac
    39241 tggcgctaac aatacaggct aaagtcgaac cgccaggcta gactttagtt ccacaacact
    39301 aaacctataa gttggggaaa tacaatgttc cagcaagaag ttaccattac cgctccgaac
    39361 ggtctgcaca cccgccctgc tgcccagttt gtaaaagaag ctaagggctt cacttctgaa
    39421 attactgtga cttccaacgg caaaagcgcc agcgcgaaaa gcctgtttaa actgcagact
    39481 ctgggcctga ctcaaggtac cgttgtgact atctccgcag aaggcgaaga cgagcagaaa
    39541 gcggttgaac atctggttaa actgatggcg gaactcgagt aatttcccgg gttcttttaa
    39601 aaatcagtca caagtaaggt agggttatga tttcaggcat tttagcatcc ccgggtatcg
    39661 ctttcggtaa agctctgctt ctgaaagaag acgaaattgt cattgaccgg aaaaaaattt
    39721 ctgccgacca ggttgatcag gaagttgaac gttttctgag cggtcgtgcc aaggcatcag
    39781 cccagctgga aacgatcaaa acgaaagctg gtgaaacgtt cggtgaagaa aaagaagcca
    39841 tctttgaagg gcatattatg ctgctcgaag atgaggagct ggagcaggaa atcatagccc
    39901 tgattaaaga taagcacatg acagctgacg cagctgctca tgaagttatc gaaggtcagg
    39961 cttctgccct ggaagagctg gatgatgaat acctgaaaga acgtgcggct gacgtacgtg
    40021 atatcggtaa gcgcctgctg cgcaacatcc tgggcctgaa gattatcgac ctgagcgcta
    40081 ttcaggatga agtcattctg gttgccgctg acctgacgcc gtccgaaacc gcacagctga
    40141 acctgaagaa ggtgctgggt ttcatcaccg acgcgggtgg ccgtacttcc cacacctcta
    40201 tcatggcgcg ttctctggaa ctgcctgcta tcgtgggtac cggtagtgtc acctctcagg
    40261 tgaaaaatga cgactatctg attctggatg ccgtaaataa tcaggtttac gtcaatccaa
    40321 ccaacgaagt tattgataaa atgcgcgctg ttcaggagca agtggcttct gaaaaagcag
    40381 agcttgctaa actgaaagat ctgccagcta ttacgctgga cggtcaccag gtagaagtgt
    40441 gcgctaacat tggtacggtt cgtgacgttg aaggtgcaga gcgtaacggc gctgaaggcg
    40501 ttggtctgta tcgtactgag ttcctgttca tggaccgcga cgcgctgccc actgaagaag
    40561 aacagtttgc tgcttacaaa gcagtggctg aagcgtgtgg ctctcaggcg gttatcgttc
    40621 gtaccatgga catcggcggc gacaaagagc tgccatacat gaacttcccg aaagaagaga
    40681 acccgttcct cggctggcgc gctatccgta tcgcaatgga tcgtaaagag atcctgcgcg
    40741 atcagctccg cgctatcctg cgtgcctctg ctttcggtaa attgcgcatc atgttcccga
    40801 tgatcatctc tgttgaagaa gtgcgtgcac tgcgcaaaga gatcgaaatc tacaaacagg
    40861 aactgcgcga cgaaggtaaa gcgtttgacg agtcaattga aatcggcgta atggtggaaa
    40921 caccggctgc cgcaacaatt gcacgtcatt tagccaaaga agttgatttc tttagtatcg
    40981 gcaccaatga tttaacgcag tacactctgg cagttgaccg tggtaatgat atgatttcac
    41041 acctttacca gccaatgtca ccgtccgtgc tgaacttgat caagcaagtt attgatgctt
    41101 ctcatgctga aggcaaatgg actggcatgt gtggtgagct tgctggcgat gaacgtgcta
    41161 cacttctgtt gctggggatg ggtctggacg aattctctat gagcgccatt tctatcccgc
    41221 gcattaagaa gattatccgt aacacgaact tcgaagatgc gaaggtgtta gcagagcagg
    41281 ctcttgctca accgacaacg gacgagttaa tgacgctggt taacaagttc attgaagaaa
    41341 aaacaatctg ctaatccacg agatgcggcc caatttactg cttaggagaa gatcatgggt
    41401 ttgttcgata aactgaaatc tctggtttcc gacgacaaga aggataccgg aactattgag
    41461 atcattgctc cgctctctgg cgagatcgtc aatatcgaag acgtgccgga tgtcgttttt
    41521 gcggaaaaaa tcgttggtga tggcattgct atcaaaccaa cgggtaacaa aatggtcgcg
    41581 cctgtagacg gcaccattgg taaaatcttt gaaaccaacc acgcgttctc tatcgaatct
    41641 gatagcggcg ttgagctgtt cgtccacttc ggtatcgaca ccgttgaact gaaaggcgaa
    41701 ggcttcaagc gtattgctga agaaggtcag cgcgtgaaag ttggtgatac tgtcattgaa
    41761 tttgatctgc cgctgctgga agagaaagcc aagtctaccc tgactccggt tgttatctcc
    41821 aacatggacg aaatcaaaga actgatcaaa ctgtccggta gcgtgactgt gggtgaaacc
    41881 ccggttatcc gcatcaagaa gtaattcttg ccgcagtgaa aaatggcgcc catcggcgcc
    41941 attttttatg cttccgccag cggcggcaaa atcaattcat cgctctcatg ctgctgggtg
    42001 tagcgcatca cttccagtac gcgcaacccc gcacggtgcg ccgcatcgtt taacgccttc
    42061 cctttcagca aaccactgat aagctgagca caaaacaggt cgccagtccc tttcaggtcg
    42121 gttttaaccc gggaatggga aatgacattc acgctgtcgg cactgaccac caccacctgc
    42181 atctcctgat tttcttcatt accggaggcg ctggtaatca ccacccattt taatgtgtct
    42241 gaaagcagac tttttgcggc agcaatggca ctgtcgagat cgcggcaatt tttaccggtc
    42301 aggatttcca gctcaaagat attgggggta attccctgcg ctagcggcag taaatattgt
    42361 cgatacgcct cgggaaggtc aggtttgaca taaattccgc tatcaatatc gccaatcacc
    42421 ggatcgacca tgatcaatag gtcaggatgg tctttgcgta gcgcagtcag ccactcggca
    42481 aggattttga tttgcgatgc cgttcccata tagcccgtgg ttacagcacg aagttggcgc
    42541 agcgcatcac gctcctgaag cgcacgcaaa taaccgctaa accattcatc cggaatcgca
    42601 ccaccgtaga aagtgtcata atgcggcgta ttgctcagca ataccgtcgg cacggcaaag
    42661 acattcaggc cgttctgctt tatagcaggc accgcaatgc tgttgcctac gctgccgtac
    42721 actacctgcg actgcacggc aacgatatcc gcctgcagtg ccctgctctt atcgttaaac
    42781 aacaacaaac tactcattta attttttctc cttgccgatg atcctcatcg tattccagcc
    42841 gaaactttac ctgattctgg cagtcaaatc ggctattaca aaacaaggat aaggtaattc
    42901 agtgaagaaa atcatttgtc tggtcgttac gctgctaatg acactccccg cttatgcgaa
    42961 gttaacggcc catgaagaag cccgtatcaa cgccatgctg gagggattag cacagaaaaa
    43021 ggatttgata tttgtgcgca acggtgatga acatacctgc gatgaagcgg tttctcatct
    43081 gcgcctgaag ctgggcaata cccgtaatcg cattgataca gccgagcagt ttattgacaa
    43141 ggtggcctcg tcgtcatcga ttaccgggaa gccgtatatc gtgaagatgc ccggtaagag
    43201 cgatgagaac gcacagcctt ttttacatgc gttaattgcg cagacggata aaacggtgcc
    43261 tgcgcaataa tcttaaatcc ccgccccctg gctaaaatgc tcttccccaa acaccccggt
    43321 agaaaggtag cgatcgccac gatcgcagat gatcgccacc accaccgcgc cagggttagc
    43381 ttttgccacc cgcagtgctc cggcaaccgc gccgccggag cttacgccgc agaaaatccc
    43441 ttcccgcaca gccagttcgc gcatggtgtt ttccgcatcg cgctgatgaa tatccagcac
    43501 ctcatccacc agagaagcat tgaaaatccc cggcagatat tccgcaggcc agcggcgaat
    43561 gcctggaata ctgctgcctt cttccggctg caggccaaca atggtcaccg gtttggattg
    43621 ttcgcgcata aagcgtgaaa cgccggtgat agtgccggtt gttcccatgc tggagacaaa
    43681 atgggtaatg cgcccgccgg tttgctgcca gatttccggt ccggtagtgg tgtaatgggc
    43741 gtaagggtta tcgggattat tgaactgatc gagcagcttt ccttcaccac gattagccat
    43801 ctccagcgcc agatcgcgcg caccttccat gccctgctct ttggtgacca gaatcagttc
    43861 tgcaccgtag gcacgcatcg ccgcacggcg ttcctggctc atgttgtcgg gcatcagcaa
    43921 tttcatgcga tagcctttaa gcgccgcaat cattgccagc gcaatgccgg tgttaccact
    43981 ggtggcttcg atcagcacat cacccggttt aatctcgccg cgcttttccg cctcgacaat
    44041 catcgaaagc gccgcacggt cttttaccga tcccgccggg ttattgcctt ccagttttaa
    44101 ccacacttca ctgccgttat ccggcgccat tcgctgcaac ttcaccagag gcgtattgcc
    44161 tattgtttgt tctaatgtac tcacgatctc tgtccatacg tggtgttgcc tgatgtgaca
    44221 cttgcgcgtc ttatcaggcc tacaggttac aaaaccttgc cattaaaaag cccggatcgc
    44281 gggaagcgcc tccgggcgtt taacattcac tcaacctatc aggcgctttg tgcgagagca
    44341 agttcctcat cgcgggtttc aatacgctcg tcgccgttat acagccgcgc atgttgcaga
    44401 ccaacgtata aacgatcgcc acgctgcggg gcatcgtcgc catgcatcac gaccgtcagc
    44461 ggttcgttgt accaccccag cggctgcacc actaattggg tgtagtgacc tttcgggctg
    44521 gcttccagta cctgtactgg tagcggcgaa tcgaggctgg tacggcggct aatatccact
    44581 tcccacgggc gcaggaagag atccaccggc ccctgatacg cgggggtgta tcccagcggc
    44641 cagcgatgtg cgccaacgtg gaactgcccg ccgcgaatgg ttccctgcag gcggtttacc
    44701 tcgcccataa actccagcac aaaacgagtc gccggttcac gccatacctg atccggtgcg
    44761 tcagcttgtt caatattgcc ctgactcatc accactacac gatcagctac ttcggtcgct
    44821 tcttcctggt cgtgggtcac gaacacgctg gtgaatttca gttcttcatg cagttgacgc
    44881 agccagcgac gcagctcttt acgcacctgc gcatccagcg cgccaaacgg ttcatcaagc
    44941 agcaaaattt gcggttccac agccagcgcg cgtgcaagcg ccacacgctg tttctggccg
    45001 ccggaaagct gcgccggata acgatccgcc agatgagcaa gctgcaccat ctccagcaat
    45061 tttgtcactt tcgctttgat cgccgccgca ttcgggcgtt cacgccgcgg caacaccgtc
    45121 aggccaaaag cgatattgtc gaataccgtc atatggcgga acagcgcgta atgctggaac
    45181 acgaaaccga ctttacggtc ccgcgcgtgc agacggctca cgtcggtacc gtggaagcga
    45241 atatgcccgc tggtttgatg ctccagcccg gcgataatgc gcagcagcgt agttttcccg
    45301 gaaccggacg gcccaagcaa cgcgaccatc tgaccagaag gaatatccag tgagatatcg
    45361 ttcagcacct gggtgcgacc aaaagacttc ttaatattgg caatctcaat gctcatgatg
    45421 ctcctcctgc tgcgcgcgtt tttcctggtt ctccaggcgc cactgcaaca tactctttaa
    45481 aaacagggtg ataatcgcca tcagcgtcaa cagcgccgcg gcggtaaagg agccgacggt
    45541 gttgtagtcc tgctccagca attcaatctg taacggcagc gaaagtgttt cgccgcgaat
    45601 cgagccagaa accaccgaca ccgcgccaaa ctcgccaatc gcgcgggcgt tggtcagcac
    45661 cacgccgtaa agcagcgccc agcggatgtt cggcagcgtg acgcggcgga acatctgcca
    45721 gccggacgcg ccaagcaaaa tcgccgcctc gtcttcctgg ctgccctgac ttaacattac
    45781 cggtaccagt tcgcgcacca caaacggaca cgtcacgaag atggtgacca gtaccatacc
    45841 cggccaggag aacataattt gcagattatg ttcatccagc cagccgccca gcgggccgtt
    45901 agagccgtag aacagcagat acaccagacc agcaaccacc ggcgacacgg caaacggaat
    45961 gtccagcagc gtcagcaata gctggcgtcc ggggaagtta aagcgcgtca ccagccaggc
    46021 cagcagaata ccgaacacca agtttaccgg tacggcaatc agcgcgatca tcaccgtcag
    46081 ccagatggcg tgcagcatgt ccggatcggc cagattttgt aacaccggca tcagtccttt
    46141 gctgaacgcc tgtacgaaga tataaatcat cggcaccagc aggatgaacg ccgataccag
    46201 catcccgatg ccaatcagaa accatttgcc ccagttaatc gggcgcgcgt cataacgctt
    46261 caattgtgtc acttccgcca ttaatgacct accacacgcc gaccaaagcg actttgcaga
    46321 gtgttaattg agaacagcaa cagcagcgat gccgcgagga tcaccgaagc aattgcgctc
    46381 gccgccgggt aatcaaactc ctgtaagcgc acaaaaatca tcagcgacgt tacttctgtt
    46441 ttccaggcga tgtttccggc gataaaaatc accgcgccaa actcaccgag actgcgggta
    46501 aacgacagcg ccacacccgc caccagcgcc ggagagagtt ccggcagcac cactttgcga
    46561 aaactctgcc agcgcgtagc accgagcgtt tctgccgctt cttcatattc cgggcctaac
    46621 tcttccagca ccggctgcac ggtacgcacc acaaacggaa tgctggtaaa ggccatcgcc
    46681 accgcaatcc ccagccatgt ataggtgact ttgatatcaa acttcgccag ccattcaccg
    46741 taaaaaccgt tcacggaaaa gagcgaggcc agcgttaaac cggcgactgc cgttggcagc
    46801 gcaaagggta aatccatcag cgcatcaagc aacgtgcggc cagggaagcg ataacgggtt
    46861 aagatccacg ccatcaacag gccgaaaacg ccgttaaaaa tcgacgccac aaacgccgac
    46921 agcagcgtca ctttataggc cgcgaccacc tgcgggttgg tgatcacctc ccagtactgc
    46981 gcccagctca tctgggccag ttgcatcacc agcgcggaga gcggcagcag caaaatcagg
    47041 cacacaaaca gcagactggt gccgaggctt aaggtaaagc ccggcagcac gcgtcttgaa
    47101 gaaactgcaa acattactta cgccccgccg ctaacagctt gtctaactcg ccgccgctgg
    47161 tgaagtgggt tttcatcact tccggccagg agccaaattt atcttccacg cggaacagct
    47221 cggtctgcgg gaatttatct ttcagtttgc ccatgacttc cgggttattt acgcggtagt
    47281 aatagtcggt gatgatggtt tgcgcctgcg ggctgtagag ccagttcagg taggcttttg
    47341 ccgctttttc cgtgccgttg gcctgtacgt ttttatccac ccacgccacc gggaattccg
    47401 ccagaatgtt ggttttcgga atcaccactt caaagccctg cgcttcatac tgtttacgga
    47461 tgttgttcac ttccgactcg aagctgatca gcacatcgcc caggccgcgc tcggcaaaag
    47521 tggtggtcgc gccacgaccg ccagtatcga acacttcaac gtttttcagg aactgggtca
    47581 taaactgttc ggttttggct ttatcaccac cgtcggcttt gtccgctgcg ccccatgccg
    47641 caaggtaggt ataacgcgcg ttacccgacg ttttcgggtt cgggaaaatc agcttcacgt
    47701 cagagcgtac caggtcgttc cagtcgtgga tattcttcgg gttgccctta cgcaccagga
    47761 agcccatggt ggagtagaac ggcgagctat tattcggcag gcgcgactgc cagtccgctg
    47821 ggatcagctt gcctttatcg tgcaggattt gtacgtcggt cacctggtta taagtgacaa
    47881 cgtcggcttt taaaccctgc aaaattgcca gcgcctgttt tgatgatccg gcatgagatt
    47941 gtttaatcgt cagtttgtcg ccaccgttat cttttgccca ttgttgctca aacggcgggt
    48001 tcagggcggc aaacagctcg cgggagacgt cataagaact gttcagcagt tccgttgcct
    48061 gtacatggcc cgccagtagc agagaagcga ccagcacgag tgagttcttt ttcagtaagt
    48121 taacggccat tgcgcaccct tataaattta acgactttct aatagccatc atatttataa
    48181 cggacgtgaa aggagtaacg gttttatata ccgtttggtg atttggaagt tgaaaaggga
    48241 ataagacgaa atcctcccca caaatgcagg gaggaaacag aggtgaatca gatgccgacg
    48301 ctaaccgtct ccggcagtgt gctgccgcca tcaatcacat tctgtgtacc ggttaaatag
    48361 ctggattcat ccgatgcgag gaaggccgcc agttcgccga cttccagcgg atcggcgagg
    48421 cgacgcatcg ggattgcttt cgccatttca gtcagcaccg attctggatc ttccgggttc
    48481 gactggcggg caatgctttc cgccattggc gtgcgaacgt atccagggca gatggcgtta
    48541 acgcgaatac cagactgcgc gtactccacc gccagcgatt tagtcaggcc aacaatcgcc
    48601 gctttcgtta aggcgtacgc cgtttcgccc gggtctgcca ccatatcgcc agtgactgaa
    48661 gacatcatca caatccggcc atctttgcgg gcaatcatct ccggcagcac cgccttcgtg
    48721 acgttccata cgccttttat attgatatca atatggaaat cgcgatcttc atcgctcata
    48781 tcaaggaaac tgcccagacg acaaacgcct gcgttattca ccagaatatc aatgcgccct
    48841 tctttttcct tagcgcgctt aatagctgcg gctaccgacg ccgggtcacg cacatcggcg
    48901 acaaccgccg tacagcgatg accacggcca cacagttcgt ccgccagctt ttcgatctca
    48961 ggggagatat ccagcaagat taggtttgcg ccatgacgcg caaaagttct ggcaattcct
    49021 tcgccaattc cctgcaatgc gcccgtaatc agtgctgtct tgcccgtgag tttacccatt
    49081 ttaatgctct ccttgttaat acagagcgtt acactccatt ccctctgaaa atacaggata
    49141 aacaaaatat tgctgcggat cagctcacta attcaggaaa atctcaattt agtcgctgct
    49201 ttatgcagca cctcaagcgt tacgtcagcg cgcaatcatt ttcagtgaca tcacatgagt
    49261 aatattcgga cttaatgatt cctttgtgtc tatcaaatcg ggatatatga tcccatttat
    49321 ctgcaattat ttgatcgcaa atattaaggt caccaccgat gcaacttgaa aagatgatta
    49381 ccgaaggctc gaacgccgcc tcggctgaaa ttgaccgcgt atcaacgctg gaaatgtgcc
    49441 ggattatcaa cgatgaagat aaaaccgtac cactcgccgt tgagcgcgta ctgccggata
    49501 tcgccgcggc gatcgatgtt atccacaccc aggtaagcgg cggcggccgt ctgatttatc
    49561 tcggtgcggg aacatccggt cgtctgggaa ttctggatgc cagcgaatgt ccgccgacct
    49621 acggcgtgaa accgggcctg gtagttggtt tgattgctgg cggcgaatat gccattcagc
    49681 acgcggtgga aggcgcagaa gatagccggg aaggtggtgt taatgatctg aaaaatatcg
    49741 gcttaaccgc gcaggatgtg gtggttggta ttgcggccag cggacgcacg ccgtatgtga
    49801 ttgccgggct ggaatacgcg cgccaactcg gctgccgtac agtgggcatt tcctgtaatc
    49861 cggggagtgc cgtttcatcc accgcagagt ttgctattac gccggtagtt ggtgccgaag
    49921 ttgttaccgg ttcttcgcga atgaaagcag gcacggcgca gaaactggtg ctcaacatgc
    49981 tctccaccgg gctgatgatt aaatccggca aagtgttcgg caacctgatg gtcgatgtgg
    50041 tcgccaccaa cgaaaaactg catgtgcgcc aggtcaatat cgtcaaaaac gctaccggat
    50101 gtaatgccga acaagcggaa gcggcgttaa ttgcttgcga gcgcaactgt aaaacggcca
    50161 ttgtgatggt actgaaaaat ctcgatgccg ctgaagctaa aaaatgcctg gatcaacacg
    50221 gcggttttat tcgcaaggct ctggaaaagg aataacccat ggctaaagag atcagcagtg
    50281 aacttctgaa caccattctt acccgggtcg gcggaccggg aaatatcgcc agttgtggta
    50341 actgtatgac gcgcctgcgt ctgggtgtac atgacagttc attggttgat cccgatatca
    50401 aaacgctgga aggcgtgaag ggcgtcattt tgaccagcga tcaggtgcag gtggtttttg
    50461 ggccaggcaa agcgcatcgt gccgcaaaag ccatgagcga gctgctgggc gaggcaccgg
    50521 tacaggatgc cgcagaaatc gccgcccaga ataaacgcca gttaaaaacc agacaaactt
    50581 ccggcgtgca acaatttctc gccaaattcg ccaccatctt cacgccgctg atccccggtt
    50641 ttattgccgc cggtctgctg ctggggatag cgacgttaat tgccacggtg atgcacgttc
    50701 cggcagatgc tcagggcacg ctacccgatg cgctgaattt tatgaaggtg ttcagcaaag
    50761 gcctgttcac cttcctggtc attctggtgg gctataacgc ggcccaggca tttggtggca
    50821 cgggcgtaaa tggcgcaatt atcgctgcgc tctttttgct tggttataac ccaactgcaa
    50881 ccacgggtta ctacgccgga tttcacgatt tctttggtct gcccatcgat ccgcgcggca
    50941 atattatcgg cgtgttgatt gccgcatggg cctgcgcccg catcgaatgc atggtgcgcc
    51001 gctttatgcc ggacgacctc gacatgctgt tgacctcatt aatcaccctg ctgatcaccg
    51061 ctacgcttgc gtacctgatc attatgccgc tgggcggctg gctgtttgaa ggtatgtcat
    51121 ggctgtttat gcacctgaac agtaatccgc tcggttgtgc ggttttagcc gggctgttcc
    51181 tgatcgccgt ggtatttggc gtgcatcagg gctttattcc tgtttatctg gcgttaatgg
    51241 acagccaggg attcaacagc ttatttccca tcctttcaat ggcaggcgca ggccaggtgg
    51301 gcgcggcgct ggcgctctac tggcgggcgc aaccgcacag tgcattacgc agtcaggtcc
    51361 gcggggcgat tattcccggt ctgcttggcg ttggcgaacc gctgatttac ggtgtcaccc
    51421 tgccccgcat gaaaccgttt gttaccgcct gtttaggcgg cgcggcgggc ggtttgttta
    51481 tcgggctgat agcctggtgg ggtctgccga tgggcttaaa cagcgccttt gggccgtctg
    51541 gtctggtggc gctgccgctg atgacttccg cgcaaggcat ccttccggca atggcggttt
    51601 acgctggcgg tattctggtg gcatgggtta gtgggtttat tttcaccaca ctctttggct
    51661 gccgtaacgt caatctggac tgattatgaa acggacaatg ctctatcttt ctctgctggc
    51721 tattagctgt agcgtcagcg ccgcaaaata ccctgttcag gcagaaagta gttgatcaag
    51781 tgtatgcggc gttaaagcag taataaaaag gccagtcagt tgtgttactg cctggccttt
    51841 aatcaatgaa tcagaaacgc ttacagcgcc atcaacttgt ccagcgacgg cgcgaagtaa
    51901 tagccgccag tgaccggttt ggtgaaacgc aacatcgcat cacgcttacc atctgtatcg
    51961 ccaaacatgc tcagcagttg ctgctcaatg ttatgcagac gcgcgcagta ggcgcagaag
    52021 tacagaccgt gagtgccact ggcggtgccg tacggcaggc tctgacgaac aatcttcagc
    52081 cctttgccat cttctttcag atcaacgcgg gtcaggtgag aggtttccgg acgttcgtcg
    52141 ccgtcaatct cttcgttggc ctctttggtg cgcccgatca tcatctcttg atcgtgaacg
    52201 ctcatccgat tgagctgctt caggttgtgc tcccaacgct gcacaaacac atagctgccg
    52261 cccgcatcca cgccgtcttt gataacagcc acttcgcggc gcgtctcttc acccgccggg
    52321 ttttctgtac cgtcaacaaa gccgctcagg tcacgctctt caacccaacg gaagccgtgg
    52381 atctcttctt tcacttcaat gcagtcacca aaggcttcca tcgccgcctg agcgacagag
    52441 aagttcacgt cgtgacgcag agaaagaatg tggatcaaca catcgaactg ggtcgtcggt
    52501 gcaagacctt taccgtagcc cggaaaatct ttcagctctt cggccccaac gccgccgctc
    52561 agagcgcgcc aggtattgtt accaaaggca accaccgcac caagatgcgc gtccgggaat
    52621 ttcgcttcaa aagttgccag tttgtcggca aatgttttac tggccgcacg cagggcgtca
    52681 acttcccctt tcacgttggc ttcgatccaa atcgccgcgc ggcaatgttc tggcaaaatg
    52741 ccactctgaa cctgagacat tgttcctcct gaaaataata atgccacgca atccgtggca
    52801 ttgttgggcg ctattttacg tgttttttcg cggcggcggt ttgttcagac gcaaattaac
    52861 gccgccagat aattttactg actttccagt ttttcagggt atcgtccgaa ggcattaacc
    52921 cttcagggcc actccattcc ccgctgaact ggtagctaat atgttgactg ccttcggctt
    52981 tacattcgac ggcacgatta tcatcactgt cggccttttg gcaattgcca aatgctttgc
    53041 tgtaaaggtc actaaacggt gtaccgattt taacaccggt gtcagcagga atatcgctat
    53101 ccagcacatc aatgcggctg atcgtgcctt gatcaccgtt aatcaccatc gccacgttgt
    53161 cgcctttcat cacttcaaaa aagcgcacca cgttgccgtt cgcggttttc attccgctgc
    53221 gcaggcgata atcgccatca agcgcatcag caatggcttg ttcttgcagt ggtgtggacg
    53281 ccgttaattc acccacgccc tgctcgctca ctttggtgga tgatccaaac cagttccacg
    53341 ggttagcggc cgaccagtta actgacgaca tcgtggaaca gccggtcagc atcaacggca
    53401 tagcgcataa cattaaacgc agcgatttca tgtcacttcc tttggttatt caataacgtt
    53461 gattggagtg caaattcacc aaaaagtgcc gttattcttt ttcttcatta aaacaagcgc
    53521 gtaaacgtcg attggtcagc agccagatta acgccacgat atccgccact accagcgcca
    53581 gaccgatacc gctaacggat tcaccgttca gccacagcca cggttgccaa caaagtaaaa
    53641 ccacttgcgc caacagcaac agaaaataga gcacatgcca gatacgggga aatgaagccc
    53701 gccgaccact cagcaaaaat gccagcaccg ccggaatacc gggaatcaac cccagccaga
    53761 aattatcgtg atcgggataa aacagattta gcagcgcagt accctgctcg cgcgacgctc
    53821 cggcaatgac aaacagcacc caggttcgcg cctgaagcaa tagcacaagc cagaagagca
    53881 agggtaaacg caggcgaccg tgcgcatcat aatggacagg atgaaactca gtactcttca
    53941 tcttcaatca aacgcttacc cagactcagc acgtcggcgt gttcatatcc caggcgttca
    54001 tacattccga gcaccatgtc gttatcttcc ggcacgttga tctgaatttt cggacagccg
    54061 cgagcaatca gctttttctc caaccgatta agcaacgcat tagcaatccc acgcccacga
    54121 aactcgggat gcacgccaag ataataagca gacccgcgat gcccgtcata accgcccatc
    54181 accgttccga ccacttcacc gttcacctca gcgaccagaa acaaactgac gtcatggttc
    54241 atcttacgct cgatgtccat ttccggatcg ttccacggac gcagcaagtc gcaacgctcc
    54301 caaagggtga tgacctcttc gaaatcttcc tggcgaaata cgcgtatctc catggtattc
    54361 gttacctttt tgcgggttaa aaggctgatt atggcgtgaa cggtcgaatt agccaatatc
    54421 tgacgaaaat cggttgaaaa agtggcataa tggggagttg tcaactattg aaatgaaaag
    54481 taaaacaatt ctcaacagca aaccgtcgta acggattacg cgatacgata taacatctgg
    54541 aactttatta ttacaactca ggccgtatga gcacttttaa accactaaaa acactcactt
    54601 cgcgccgcca ggtgctgaaa gccggtttgg ctgccctgac gttgtcagga atgtcgcaag
    54661 ccatcgccaa agaagaaccg ttaaaaacca gtaacggaca cagcaagccg aaagcaaaaa
    54721 aaagcggcgg cagacgtgtc gttgttctcg atccaggcca cggcgggatt gataccggag
    54781 cgatcggacg caacggttcg aaagaaaaac atgtggtgct ggcaattgcc aagaatgtcc
    54841 gttccatatt gcgtaatcat ggcattgatg cgcgtttaac gcgttctggc gatacgttta
    54901 tcccactgta cgatcgcgtt gaaatcgccc ataaacatgg cgcagatctg tttatgtcaa
    54961 ttcatgccga cggctttacc aacccgaaag ctgccggtgc ttcggtattt gccctctcta
    55021 accgtggggc aagtagcgca atggcaaaat acctgtctga acgcgaaaac cgcgccgatg
    55081 aagttgccgg taaaaaagcg actgacaagg atcacctatt gcagcaagtg ctgtttgatc
    55141 tggtgcaaac cgatactatt aaaaacagcc tgacgcttgg ctcgcatatt ctgaagaaga
    55201 ttaagccggt gcataaactg cacagccgca acaccgaaca agccgcgttt gtggtgttga
    55261 aatcgccgtc ggttccgtcg gtgctggtgg aaacctcgtt tatcaccaac ccggaagaag
    55321 agcggctgtt aggcactgca gcgtttcgcc agaaaatcgc cacagcgatt gccgaaggcg
    55381 tgatcagtta tttccactgg tttgacaacc agaaagcaca ctcgagaaag cggtaagtga
    55441 tgaaacccga cgcacaccag gttaaacagt ttctgctcaa ccttcaggat acgatttgtc
    55501 agcagttgag cgccgtcgat ggcgcagaat ttgtcgaaga tagctggcag cgcgaagctg
    55561 gcggcggcgg gcgtagtcgg gttttgcgta atggtggtgt tttcgaacag gcaggcgtca
    55621 acttttcaca tgtccacggt gaggcgatgc ctgcttccgc caccgctcat cgtccggaac
    55681 ttgccgggcg cagtttcgag gcgatgggcg tttcactggt agtgcatccg cataatccgt
    55741 atgttcccac cagccacgcg aatgtgcgct tttttattgc cgaaaaaccg ggtgcagagc
    55801 ccgtctggtg gtttggcggc ggcttcgatt taaccccttt ctatggtttt gaagaagacg
    55861 ccattcactg gcaccgcacc gcccgtgacc tgtgcctgcc atttggcgaa gacgtttatc
    55921 cgcgttacaa aaaatggtgc gacgaatact tctacctcaa acatcgcaac gaacagcgcg
    55981 gcattggcgg gctgttcttt gatgatctga acacgccaga tttcgaccac tgttttgcct
    56041 ttatgcaggc ggtaggcaaa ggctacaccg acgcttattt gccaattgta gagcgacgaa
    56101 aagcgatggc ctacggcgag cgcgagcgca attttcagct ctaccgtcgc ggtcgttatg
    56161 tcgagttcaa tctggtctgg gatcgcggca cgctgtttgg cctgcaaact ggcgggcgca
    56221 ccgagtcgat cctgatgtca atgccgccac tggtacgctg ggagtatgat tatcagccaa
    56281 aagatggcag cccggaagcg gctttaagtg agtttattaa ggtcagggat tgggtgtaac
    56341 tccctcaccc ccactcccgc atccgctgat gcagcgtcag tgacggcttc tcggcaaaca
    56401 gttgctgata atccgtggca aattgcccaa gatgccagaa tccccactgc atggcggcgt
    56461 cttttaccgt tgtgctttgt gaccacggac ttatgagttc gcggcgcacg gcgttcaggc
    56521 gaatacgttt cagccacgcg ttcggaccaa tgcccaaaat ggcgtgaaat gcgttttgta
    56581 atgtgcggcg gctgacgtgc aattgattgc acaagtccag caccgtcacc ggttcggaca
    56641 tgttttccag cacatattca cgggcgcggg aaagcaatcg acggtaactc tgatgactga
    56701 tgctttccgc cgtcaccatt ggctgcgctt cttccagcat cgcccccatc gccattagca
    56761 aattatcccc cagcactttt cgcactgcag gctgatggag attttccggg ttttcgcaaa
    56821 acgtcgccag cgcctgttgg acaaagcccc acagagcggc tttatgctgc tctttcactt
    56881 ccagcgccga ctggctacgc agcatatgta atacccgatc cggattatgc aaaaagttag
    56941 cctgacgagt gatgacatct tcagaaagca ctacgccaag gatcgtgtaa tcgtccggcg
    57001 tacttaattc aaactcggtt ccaccaaggc gcgtcgcaat ttctgcgctt cccagacatt
    57061 gcgaaccgat aaatccctgc tcaccgcgcg tcgccggaat gccaaaccag aacgagttcg
    57121 gccagaccag gcacgactga cgcagcgcca gaccagtgta ttcacgaaaa acctgaatat
    57181 catcgagtag aatttccgta aattcaccat gaaacttgcc cggatgcagt tgatcgtaaa
    57241 tctgctgcca ggcggtaatt gtcaaagcat gttcatagac gtccgttgtc tgtcgttgat
    57301 gaacattatc cacttcgacc ttcggcgtga gcttcaggtt ttcgggtaag ggttcatgat
    57361 aaagatggtg caaattggct gtacgggtct ttttcatgat gttaatgccg ggtgttgtag
    57421 gacacccggc acctccgaca ggttaatggg gcttgagacg gtaacgacta ctgcgtttac
    57481 gtaacgtccc ggcagaaaag agctgttcga gcgcatttct ggctttttcc agcggccagc
    57541 caaagtgggc agccacttct cctgccgtca ttccctgacg tactgatgtt aacagcgcca
    57601 acaactcgtc agcagattcc gcgacgataa ctggcgcgtc gggttccttt acgggttgct
    57661 tcggctggcg gttaaagcca gtaaccagcc actgagtgtc atcgtctggc cgaccgatct
    57721 ccttacggct gatgacacgc ccggtacgca tcgcggcagc acaacctgcg tccagcgccg
    57781 cacgacacgc cgccagatcg ccttccacca ccagcgttag gcgaccaggg tcaagcactt
    57841 cgtgactgag cagacgaacg ttagctgctt tgagcatggc atcagctgca tctatcgcgg
    57901 cgaccatgcc gtccacttcc agcaatccca gtgcattgat catcggcaac ctccgttacg
    57961 cacgctggat tggattacgc gcgatttcca gcactgcatc ggtaaaggcg ttacaggctg
    58021 ctttacacgc ggcctggcta ccggttaaaa atgctgccga gtagttggtt tcagacggtg
    58081 gcgggacata ggtcaccaac tgcacgtcgg cagatttcaa cgctgcatca atgccgtagg
    58141 tcgcttccag cggcggtgcc accagatacg ccatcggatc gccaggcgtg atcccggcgg
    58201 ttgatgagag ataagaaccg gtacgcgaaa ctacatgcgc gaggaatgcc gtatcttgct
    58261 tatcgttcgc ccactggaaa gcagcgccat tttcaatatt cgcaaccatc gcatccagac
    58321 cagcacgcac ttccgccggg tttggtccac caagcataat cagcacctca ccggcagtcg
    58381 gtgacgggcc gtgtgcagcg cccgcataca gcgagcgacc atataccact tcgaccatcg
    58441 cctgcttggt cgcttcgtca gccgcaatgt aggtgacgtc atctgaatca gcagaaatca
    58501 gcccgaggct acgaatatgc ggcggcaatt tcagttcacg tgcaaattcg gcgttaacgg
    58561 aggcaatcac ccgcatggcg gttaccgacg gtcgaatcaa atctaaagct ggcatgatgc
    58621 ctccttatcg ggtcatgttg atgccggatg ctttctgctc cagcatacgt ttcgccaaat
    58681 ccacaatgac ggctgcggct tcaaccggcg gcgtgccgcc ctgatgaatg ttagaaatac
    58741 aggtgcgatc ggcctcaacg gtggtcgcca tacgcggcga ataaacggca tagcaggaga
    58801 ggctttctga ctgccccaac cccggacgtt cgcccaccag caggatcact actttcgcgc
    58861 cgaggatctc accgatctga tcttcaatct tcacgcgacc ataacgtaca aagaatggcg
    58921 taccgacttt cagcccggct tgtttcaggc ccgccatcag cggcggcagg atctcttcgt
    58981 agttcacggt gatcgcatca gttgacaggc catcagaaat aacgacctgc acgtccggat
    59041 tagcaacaca ctgtgctttc agcgcttcaa cagcttctgc acacaggcgg cggcccatat
    59101 ccgggcgagt caggtagaga tttttgtcgc tgatttcgga gcggacttcc agcaagccct
    59161 gcgctttcac ccactcttcc ggtacttctt tcagaacggt atctttcgaa cgagagtgat
    59221 cggccaggaa acgcagcaac gcctgggtac gcggacgcgg accggcacga ccggtacaaa
    59281 cgcgggccac ggtgctgcga cgcagttctg ttaatacgtc tgcgcgatgc gggttttcaa
    59341 caccaatcca cgcttttgct tccgcagaac ctaaatccag cgcgcagctt tccgaggtca
    59401 ccggtgccgc acagttggtg gtggcgcact ttgcttctga cggcgcgggg gccgtttgtc
    59461 ccattgacgc catcacactg cgtacaattt cttcaatctg tttttgatcc atgatgtgtt
    59521 atctccgcgt catcagaaga acagtgacgg atcgcccgcc cgtttggtca ggcgaccgtt
    59581 tgccataatg cccatgcttt ccagccagcg ttcaaactcc ggtgacgggc gcagattcag
    59641 taactgacgc acagtggcag tgtcgtggaa tgccgtggtc tgatagttga gcatgatgtc
    59701 atcgcccagc ggcatcccca tgatgtagtt gcagcctgcg gtggcgagca ggatcatgag
    59761 gttttcgttg aggttctggt cagcgtcagc gtggttggtg tagcagcagt cacagcccat
    59821 agagatgccg ctcagcttac ccataaagtg atcttctaag cccgcacgga taatctggcg
    59881 gtcgttgtag agatactccg gcccaataaa gccgaccacg gtgttgacga taaacggatc
    59941 gtaatgacgc gccagcccat agttacgtgc ttccatcgtc acctggtctg cgccgaagtt
    60001 agcgccagcg gacagcgcag agccttgtcc ggtttcgaag tagaggcagt tttccccggc
    60061 gatacggttg aactccgcgc ccactgcgcg cgcttcatcg agcatcgcca gctctacgcc
    60121 aaactctttc agcccttttt cgctgccgca gatactctgg aaaatcagcc cgcctggtgc
    60181 gccacgacgg atcgcttcga tctgggtggt gacgtgcgcc agtacgcagc cctgagttgg
    60241 gatgttgaat ttgtcgatca cgccataaat ggtatccaac acgcggctta agttttccac
    60301 gtcgtcagtc accgggttaa cgccaatcac cgcatcgccc accccgaagg aaagcccttc
    60361 gtagatttgc gcggcgatac tttgcacgtc gtcacgggta tcgttcggct gcaaacgggc
    60421 gctaaaggtg cccggaatac cgatggtggt attggccttt ttgatcaccg gcattttctt
    60481 cgcgccgtag atcaggtccg cgttggagca aatcttcgct accgccgcca ccacttccga
    60541 ggtcagccct ttgcgggtaa aggcaatgtc gtccacgctg atttcatcgc tcagcacata
    60601 ctcacgcagt tcgctgatgc tccagttttt aatctggtta taggccgttt cgttaacgtc
    60661 gtcctgaatc agccgcgtca cgcagtcatc ttcataggca atcaccggat tattgcggat
    60721 gtccgctacg gtcatttccg acaacacctg ctttgccgcc acgcgctcct gtgaacttgc
    60781 cgcagcaacg cccgccagca catcccccga acgcagttcg ttggctttag ccagcacctc
    60841 ttttacatcc ttaaactgat atacattgcc gaacaatgtg gtctttagtt tcataagtcg
    60901 ttccctcagg aaggaaatgc gagtgatttc accgtcaccg gcacaaccga tccgccaaaa
    60961 agaggcgtac caatgtcgat atagtccccc gcgcggacaa tcacttcgtc aatgactgcc
    61021 aacgggagtt gttgtagctg tgggcgcaac aacatgccca gagctttacc aaagtcctgc
    61081 ccggccacca ccagcagggg atgcggattc ggaaaacgcg cgacgaaatc gaccagcgcg
    61141 ttgatgaccg tcagtaccgc ggcgtaacgc acaggcagcg aggcgggaag cgccagcacg
    61201 tacgcgtcag ttttggggca aagatccagt tgaatcagcg cctgttgcca ggcactgacc
    61261 agatccgttt catcaatcgg gatcgccacc ggcaaattgc gcagcggcag ttgtacgccc
    61321 tccagccaga ttgtgctgcc agagagcgaa agggtatgtg cacccgcgcc aatcaccgtg
    61381 gcgcgtacgg tttgcgccgg aaactgcacg ttcatctcac gcaggcgcgg atggtcatgc
    61441 agcgccgttg ccagcagcgg gccgatatcg gcaaaacaga acgggtcggc gggctggtgg
    61501 cgataacatt cgcccacgcc gccagaaagc gtaatgattt cgggcgtaac acctgcgggc
    61561 agcaaaccgg tttgcatcaa tgcctgcgcg agcggcgaga gcgttccgtc aatcacttcg
    61621 acaatcagct ctgccatccg ccgggtaacc tgcaccagct gcgcgccggt cagcgaacgg
    61681 gcgtcagtgc ctgcaccgaa gcactcatcc acaatcatct gccccggttt atgagcgtaa
    61741 accacgcgcc cctggctgtc ggtttccagc aggcgaccgc cgacgttgag gcaggcggtg
    61801 ccgctgattt ttccggcatc gaacagggcg tagttcgcgg tgccaccgcc gatatcgata
    61861 ttcaggaccc gacacagccg ttgttcagaa agggtttgcg ccccggcacc gtgtccggcg
    61921 atcacggatt caagatgcgg cccggcgctg gcgacgacaa aatcgcccag cgaccgggag
    61981 agcgccatca ccgccgggcg agcgttgcgg gtttttgcgc tttcaccggt gataatgatg
    62041 gcaccagaat caacgctttc cggcgcaatg cccgcagcct gatattgctc gaggattaag
    62101 gttttcagtt ccgcttcttt taaaccaccc tgtttatcga caggggtaaa gaacaccggg
    62161 ctttgccagc taatttcgcg tttgatgaat tcgtagcgcg gcacctgcga caccgccgca
    62221 cggttaacca gctccaggtg ggagaagatc acctgggtgg tggtggtgcc gatatcgata
    62281 ccgacgctca gtagctggcg agtgttcacg attgcgcctc cgcttcagtt ttggccgcgg
    62341 tcgcgtcttc ttttggcacc agcatcatcg ccacgccaat cgccgttacg ccgccgatca
    62401 acttgccgac aatcatcggg aaaatcatgg cgttcatgtt ggcagcggcg aagcctaagt
    62461 ggtcacccag ggcgaaagca gcggaaacgg cgaaggcgca gttgatgact ttgccgcggg
    62521 tatccatctg cttcatcatg ccaaacattg ggatgttgtt ggcaagcgtt gccaccatgc
    62581 cggctgccgc gatgttgttc atatttagta ctttaccgac gctcatcagc ggtttttcaa
    62641 accagcgagt cagcagcagc accatcggat acgcccctaa cagaacgcag gagatcgagc
    62701 cgataacttc aatggcgcgc atcacttcac cgggtttatc gccaggggcc ataaagatag
    62761 gatcaaggcc cgggatcagt tcccagccaa ggagaaattt cactaccgca gcggcaagac
    62821 cgagggtgat caatgcaacg aggaatttgg cgaagatctg gaagccgttg atcatttttt
    62881 ccgggatgaa tttcagcccc agcgccacca gcaccgcaac gataagtacc gggatcatgt
    62941 tcatcaggat cagcgcaaag gtgaattcaa caggctggcc gttgatctgc acaccggagt
    63001 acatcgctac cagaccacca gcgatacagc caatcggaat ggtcactatg cccgccagca
    63061 cgccgagcgc cagataacga cggtcagaag gttcgataat gccgagcgcc accggaatgg
    63121 aaaacacaat cgttggcccc atcatcgacc cgagaattaa cccagagtat agccacgctg
    63181 ctacgtcacc gcccgccagc tctttggcga ggaagaagcc gcccatatcg cacgccagca
    63241 gtgttccggc gaacattgac gggtttgcgc cgagcatttc gtaaaccggg ataatcaccg
    63301 gcccgagtac gtgggccagt accggtgcca gcgcggtcat accgaccatc gccaggccca
    63361 gagcgcccat tgccataaag ccttcttcga actggccgcc ggccccttcg atacttttgc
    63421 cgaacttacc gaggaaacga gcagaaccgc cgaactgcga caggatcctg tctacggcag
    63481 ctatcagcat aaagaacatc atgatgtaca tgatgatttc gttaataccc atcgccttta
    63541 ctccctgtta gttgttattt attggcggat gcggcgtgaa cgccttatcc gccctacatg
    63601 tgcaatcccg aaggccagat aagacgcggc aagcatcgta tccggcaatt gcaccgcgcc
    63661 actggcggat gcggcgtgaa cgccttatcc accctacatg tgcaatcccg tcggccggat
    63721 aagacgcggc aagcatcgca tccggcaatt gcaccgcgcc actggcggat gcggcgtgaa
    63781 cgccttatcc gccctacatg tgcaatcccg tcggccggat aagacgcggc aagcgtcgca
    63841 tccggcaatt gcaccgcgcc actggcggat gcggcgtgaa cgccttatcc gccctatatg
    63901 tgcaatcccg taggcctgat aagacgcggc aagcgtcgca tccggcattt attgcgccgc
    63961 tgcgtacagg ccgacaatct gctccaggct ggcggtacgc gggttgctgc gcagacaaat
    64021 atcttcctgc gcggcctgcg cccatgcacc gtaatgcccg gttgttgcac ccacatcagc
    64081 cagccgcttg ttgatcccaa cttccgctat cagctcactt accgcattaa tagcgtcacg
    64141 atcgtcggat tttttagttc gcagcgcccg accaacctga ctaaagcgtt cacgacagac
    64201 catccggttg aactccatca ccgttggcag caacatggcg ttcgccagac cgtgcggaat
    64261 atgcagcgcc gcccctggct gatgcgccat cgcgtggcat aacccaagcc cggcgctgga
    64321 aaacgccatt cccgccatac atgaagccag caacatgctc tcgcgggcga caaggtcgtg
    64381 accgtagccc accgctttcg gcagcgattt gccaatcatc gcaatggcac caatcgccag
    64441 gctgtcggta aacggtgtag cgttcagcgc gctgtacgct tcaatggcat gggttaacgc
    64501 atcaatgcca gtcatcgccg tgacatgcga cggcacgcct tcggtcaacg cggcgtcgag
    64561 aatcgccaca tccggcatca gcgaggcatg ggctaacacc tgtttgcgcc cgcttaccgc
    64621 atcgataatc accgtcacgt tagtcgtttc agagccagtt ccggcggtcg ttggaatggc
    64681 aatcaacggc aggcgcggtt gcagaacgct ggtttctgac atttctgcca gcgtgctatc
    64741 cgggttcgtc accagcaacg ccacggcttt cgccgcatcc agcaccgagc cgccgccaaa
    64801 cgcgatcacc ccgtcacaac ctgattcacg caactgcgcc acggctgcac acacgtcggt
    64861 gatgcacggt tcgcccaccg gacatggcca gagcgtcatg gcgatgcctt taacggccag
    64921 gctacgtgtc agcccggcgg tcatccctgc ctgatgcaaa aagctgtctg ccatcacgaa
    64981 cagatgtttc aacccacgcg tttgcgcttg ctgcccgcaa ctgctcaccg cgcccggacc
    65041 gcaaagcgtc accggtggaa cgctaaatgt ttttacccgt tgcagattca gggtatcgaa
    65101 cgcctgaaaa agcgccgttt gcaattcact ttgcatagat cccctccgct ttctctcgcc
    65161 cactactggc gatcgccagc ggggtcataa acaaactgtg ctgcggtaaa tgcacctgta
    65221 acgccggaaa ttgtttgcga aacagttccg ctacgcccgg ttgcatacag gaaccgcccg
    65281 ccagccataa atccgtaatg ccctgccctt caatatgccg ggccacaatg tccgccattt
    65341 tttcgtacac cggtttcacc gcgggccaaa tctcgtcacc gtgaccgcgc ttgtactgct
    65401 ctgcctcttc cagcgaaata cggcgatttc cggcgagagt cagagagatg tgatgtccgc
    65461 cggtcgcttc atccgccgag tacgtcacct tgcccttttt cacgatggca ataccagtgg
    65521 tgccaccgcc gatatccact acaccggcgt tgtccagttg cagcagatcc gcgactgcgg
    65581 ttggctcatc cagcacatgg ctcacttcca gtccagcaga ctccagcacg ttgatggaaa
    65641 tacgcgggtc cgtacctgga ggaaaagagg tcgccgcatg gctaaaacgg cgaccgaatt
    65701 gctgctcgag ggtgtcgaga tggcgacgaa caatggtgac cgcaccgaag aaatcccaga
    65761 cgatgccgtc gcggaccaca tcagcccagt cgaggcatac cgccaccggc tgaccgtcgc
    65821 ggtcgacaac catcgacacc acatcgcaag tgcccagatc cacccccagc cacagcggag
    65881 attccgtcgc ggcaggcgtc tggttacaca gcgttgccgc tatgtgcaga cgtggggtga
    65941 gccattgttc gtcgtgcgcc atcggttact ccttaaacaa tgcgaaacgc atcgactaat
    66001 acacagcgac gcagacggac aaacgtacac gcgctggtta ccccttcacc ggttggcgtg
    66061 gtgatggtca tggtggtcca gccttccccg cccagcccca gcccggcaat gcacggtccg
    66121 ttcttaacga aaatgctggt atcaatggca ttcgccatct ggttcatgtt ctcgatattg
    66181 cgcgagtgca ttgccgccgt gtggtggcaa ccgccttcca gtttcaccgc cagcgcaatg
    66241 gcatccgcca cgttggcgac gcgcacgacg ggcagcaccg gcatcatcag ttcggtcacg
    66301 gcaaacggat gttctgcggt ggtttctaca aacagcagtc gcgtttgttc cggaacttta
    66361 aggccgattg ccgccgcgat tttcgccgca tcgcgaccaa cccagtcacg gctgacggtg
    66421 ccttttccgc gctcgtcgat atttttcagc aacaccggtt gcagttgctg cgcctgctct
    66481 gcggtcagtt tcaccgcatg ctggccttcc atcagacgca tcagttcatc ggctacgcta
    66541 tcaacaacaa tcagtacctt ttcgtcggca caaatgatgt tgttatcgaa agaagcgcct
    66601 ttgacgatgg actgagcagc acgggcgagg tcggcggttt catccaccac taccggcggg
    66661 ttgccagcgc ctgcggcaat cagacgttta ttggtgtgtt tacgcgccgc ttctactacc
    66721 gcttcgccac cggtcaccac caaaaggccg atgcccggaa acttgaacaa gcgttgggcg
    66781 gtttcgatat ccggatttgc tacggtaacc agtaagtttt ccggcccgcc tgcggcaaca
    66841 atcgcctggt tgagcagcgt aatcgcccgc tgggagactt ttttcgccgc cggatgcggg
    66901 gcaaaaatga cgctgttgcc cgcggcaatc aggctgatgg cgttgttaat tacggttgcc
    66961 gccgggttag tggaaggcgt caccgaagcc actacgcccc agggtgcgtt ttcaattaaa
    67021 gtcaggccgt tgtcgccagt cagcacctgt ggagagaggc actcaacgcc tggtgtgccg
    67081 cgcgcctgag cgacgttttt ggcaaattta tcttcaacgc gccccatgcc ggtttcactg
    67141 acggcaagtt ccgctaaatc tctggcgtgt ttttcgcctg cttcacgaat ggctgcaatg
    67201 gctaactggc gcattgccac gctttttaac ccttgctggg cgactttggc tgccgcaacg
    67261 gcgtcatcca gggacgcgaa aacgcccatc tcatgaacgg cggtgggcgg cgtgtcactg
    67321 ctttgcattt tcagcagtac cgctttcacc acctgttcaa tatcctgttg attcatgatg
    67381 ttctgcctta tttgtggaaa atgacctgac cgccagacac cacctcatcg acaatgccaa
    67441 tcacgcacag atcgaccggt gacgtttcgc ttttatgcgc ctggcgggcg gaactgccac
    67501 tcaccagtaa cacccactcc ccggttcccg cgccaatatt gtcgatggcg acggcgcatt
    67561 gcccgtcagg attaccttgt ggatcaatca tttccaccat cagcaatttg tcatgcgcca
    67621 gtccgtgatg gcgtacggta caaacaattt gtccagtgac gactgccagt ttcatacccg
    67681 cctccgtggc gtatttcagg taaaagctcc ccctaccctc cgcagaaggt aaaatgaaaa
    67741 aggagagagc gtgacgcccg aatcgacgtc acacagggtg attacaggtt gctgctatcg
    67801 cctttcaggc cgatcgggaa gacttcttcc aggtcgccgt gcggacgcgg gataacgtgt
    67861 acagatacca gctcgccgat acgctgtgcc gctgcggcac ctgcatcggt cgcggcttta
    67921 caggctgcaa catcaccacg aaccatggca gtacacaggc cgccgccaat ctgcttaaca
    67981 ccaaccagct tgacgcgcgc agctttaacc atcgcgtcag aagcctcaat cagtgcaacc
    68041 aggccccggg tttcgatcat tcctaatgct tccattgtgc tttcctcttt atcagggtcc
    68101 agaacgggac cgttcattca accagtgttt gtaaactgct ttcgcggttc acttctgtct
    68161 gacgcggcac agctgccacc agcgccagtt cgataatttc ctgcacgcta caaccacgag
    68221 agagatcgtg catcggcgcg gcaagtcctt gtatcaatgg cccgacggca cgatatccgc
    68281 caagtcgttg tgcgattttg tagccaatat ttcctgcctc cagcgacgga aaaaccatca
    68341 cattagcttt gccctgtaac gggctggcag gcgctttttg cgccgccact tccggcacga
    68401 aggcggcgtc aaactgcaac tcgccatcga caaccagctt tggtgcgcgc tcacggacga
    68461 tttctgtcgc ctgctggacg ttggcaacgc aggggtgacg ggcactaccg ttactggaaa
    68521 acgacagcat cgccacgcgc ggttcttctc cggtgatggc gcgccaggtt tcggcgctgg
    68581 caagcgcgat atccgccagt tgtgccgccg tcggctgcgg caccacgctg caatcggcaa
    68641 agcccaacgc cggaccgctg tactgtggca gcatcaggaa aatggatgag agcgttttac
    68701 agcctggctg caagccgata atgcgtaatc cggcacgcag cacatttgcc gtggaagaga
    68761 ggttgcccgc gatacagaca tccgctttac cggcgctgac cattgcggcg gcgaacatca
    68821 gcggatcggt aagtttttcc agcgcatccg gcggcgtttt ttcacccgcg cgggccagcc
    68881 agcgatgagc aaattcttcc cgcattgcga ggttgccatg cggatctatc acctgtagcc
    68941 cgtccatcgc cacgccgtga ctgagcgcaa actgacgaag ttcaaacgga ttggcgacca
    69001 gaatgggcgt tgccagacct tgttgatgta aatattgcgc agctttcagc acacgttgat
    69061 ctaacgcatc cggaaaaact actctggcgg gcgctcgcaa cgccagttca cgacaacgtt
    69121 taataatcat tgcgtttccc ccagtcgttg ttggatctgc ttcaccgtta gcggctgctt
    69181 cgttacgctc aggatcatca tcacgtagac cgtgctggaa aggcggttca gcgcctgcaa
    69241 aatgtccggg cgtaatactt caaagctgcg ggtgataaag acctgcgccg ctacggtttc
    69301 ggtttcccgc actttggtgc gtaacagatt cagcaacgcc gcatcgcgcc cgtggctggc
    69361 ttccggcacc agatgatcgt ggtcgagata acgcagcggc tggtgagaaa gccgatgcag
    69421 atcttcgtca gaaagcccga caatcgcctg acagcccaac ggttccccca gcgcatcggc
    69481 acgcataatg ttgccaagac gcgaacggat atccgccagc cacggctgcc acggttccgc
    69541 cagttcaatt tgcagccaca ctgccagcgc aatggtgctg tcgagaacag cgcgaaaacc
    69601 cagacgcgga tcgcttttgg cgaccatttt ttccgccgac aggtgagtca gcgtatccgg
    69661 cttttttgct accggctggc ggcacagttc gcagcacgcc tgcggatgtt catcgctact
    69721 ggtcagcccg tgaacgggct gcggctgctg ctgttcatcg tcaacaaaca ggcggccctg
    69781 ctcgtcaata aacttgatgc gcagatggcg gctttccagt aactcccggg cagagggcgt
    69841 caggcgactg tccgcaggga gatggatctc tgcgccttcg ctgagcgtat ggttcgctct
    69901 tagccatgct tcggtgatga aatctttcat agggattgcc agttagccgg ccaggcgaca
    69961 tacaggaatt tcacgctgga tgtcgtacca aattcgatgc tggagccttt cgggataaac
    70021 atcacatcgc ccgctttggc gatcatagtt tcgccttcgt ggcgaacatg cagctcccct
    70081 tccagcacca tgtcgatttc gtcgtagttc agggtccacg gaaagaaggc gttctcccac
    70141 tgcatgaaac cagcggccat actgctgccg tcgtctccgg tcacgagatc agtcaagcct
    70201 acgcagtgcg gttgtgcgcc gtcgaaacga ccaaacttga cgctgctgcc atcaataact
    70261 ttgatgccgc ctttgccggt caccgattta aagctcggct gcagtgcacc ctgttccagc
    70321 gactgttttt ccttcatcac tttttccatc agctgcgcga ccaggctttc ggtaaactgg
    70381 ccttccggta gctgggcgat gatggtttcg cggatgcgct ggctttcggt tttatcggct
    70441 ggcacgctag cgggaacaga cgccgttacc gggatcgatt catcacattc ggtgatggta
    70501 aaacccaaca gatcagccac ttcacgagct tccggggtaa taatgctggc gcgcagaacc
    70561 acggacattg ccagttcgcc gcgcgcgtgt gcttcacgaa tatcattcgc tgtgatgagt
    70621 tttttcacct gcttcctcct gtttggtgag agatgccaga taatccacca gttgctgcac
    70681 gctttgcggg tcgtggctgt ttagctcgaa aatcggctct tcgaagcccg tctcaagcaa
    70741 taactttcgc gttgcggcga cgtcagcatc tggcatgtcc gttttactga tgacggcgat
    70801 ttgccgtttg ctgacgccaa tatccagcaa cccggcaggc aaccgacttt ccgggtcatt
    70861 cgcgccgtgg acatagatca gcatgtccac atcctgtagc gtggtaatta aggcgtgata
    70921 ccagcgggga tggctaaaat attcacccgg cgtatcaata tcgccattat cattcaattc
    70981 cacggcctgt gtttttcttg cgagggtata attcccctgc aacgcattaa ataacgttgt
    71041 ttttcctgcc ccgacggtac ccacaaacgc aattcgtttc atgaatgccc catattaact
    71101 tttggtcatt tcacaaagcg tataatttaa taagcgtcct aaaccactga cggtttgtga
    71161 taacgcttct tctactgcgc ccaccgaacc ataaatcacc agcgcgccgc taaaacggtc
    71221 gagaaaaccg atatgcacat cggcggcttt caacgctaaa tcaccggcga tcatcgccgt
    71281 ttctccggga gttagcgtca taatgccgat tgcgcccgca tccggaacgc cgattttctt
    71341 cgccagttcc tcgccgggat gcgcgatgag atgcgccagt gtgacctgtt taccgggcac
    71401 aaattcctga atgatgcgtt ctttatccat gagtcgccgc ctgtgaaaaa ctttcgtgta
    71461 tcgtgacaat ttttcgtcag agtaaataac aaaattcggc aagcacgttt aaaaggtgag
    71521 gtagatcact aagaatggag agatatttgc tgtggtgcat aaactttcat atgcaacgca
    71581 tgaatataaa taaaataata tcgagatatt ttatatactg atttttttat tattcgcaaa
    71641 ctcagtaata aactttacta atatattgca gctaaaccgc ttaccctgaa tattcagggt
    71701 aagcgtgaga gttaaaaaag aattacagcg gttgggtttg cgcttcaacc acggccagcg
    71761 ccaccatgtt gacgatacga cgtacagatg cgatcggcgt taacacgtga accggtttcg
    71821 ccacgcccat cagcaccgga ccgacagtca caccttccga gctggaaacg cgcagtaagt
    71881 tgtaactaat gcgggcagct tccatgttcg gcatcaccag aatattagcg gaacctttca
    71941 aagggctgtc cggcatacgg tcgttgcgaa tcgcttccac cagcgctgca tcgccgtgca
    72001 tttcaccatc aatcatcagt tctggcgcac gttccctgac cagttccagc gcctgacgca
    72061 ttttgctcga cgacgggcag tcagaagaac caaagttgga gtgcgacaac agagccacac
    72121 gcggttcaat accaaaacga cgaacagttt ctgccgccat caaggtgatc tccgccagct
    72181 cttctgcatc aggctcatca ttgacgtagg tatcggcaat aaaggtgtta ccactcggca
    72241 acagcagcgc gttcattgca cctgcggtgt gaacgccatc gcgataacca aagacatttt
    72301 tcaccacgct aaagtgttca tgataatcgc ccaccgtacc gcaaatcatt gcatcggctt
    72361 cgccacgctg aaccatgatc gcgccgatca ctgtcgggtt actgatcagc gcacgctgcg
    72421 cctgctcctg agtgacaccg cgacgcttca tgatctggaa gtattcggtc cagtactctt
    72481 taaagcgcgg atcggattcg ttattgacga tctcaaaatc aacgcccgcc ttgatctgca
    72541 agcccagttt ctgaatgcgc atttcgatca cgttcggacg accaataagg atcggtttcg
    72601 ccagtcccag cgttaccagt tcctgagtgg catgcagaac gcgcgcctct tccccttccg
    72661 gcagaactac gcgcttcggc gctttgcgag cctgggagaa aatcggcttc ataaacaggt
    72721 tagttttgta aacgaactct gtcagcttgt cgatgtagac gtcgaaatca gcaatcggac
    72781 gcgtcgccac gcccgacgcc atcgccgctt tcgccaccgc aggagcgatc ttaacgatca
    72841 agcgcggatc aaacggtttt ggaatgatgt attccggacc aaagctcaga tcctgatcgc
    72901 catacgctga agccaccact tcactctgtt ccgcatgggc cagttctgca atcgcgcgta
    72961 ccgccgccag tttcatctct tcgttgatgg cggttgcgcc aacgtccagc gcgccacgga
    73021 agataaacgg gaagcaaagg acgttgttca cctggttcgg gtagtcggaa cggccggtac
    73081 agatgatggc atccggacgc acttctttcg ccagcggcgg cagaatttcc ggttccgggt
    73141 tcgccagcgc caggatcatt ggcgcacggg ccattttctt caccatttcc tgggtcagca
    73201 ctttcgggcc ggaacagccg aggaaaatat ccgcgccttc aatcacatca tcgagggtac
    73261 gtttaccgtc atccaccacc gcgtatgcgg ctttggtttc cgccatgttt ggctcacggc
    73321 cctgatagat aacgcctttt gaatcgcaaa ccacgatgtt atgtttttgc agacccagcg
    73381 ctaccagcag gttcatacag gcgattgctg cggcacccgc gccagaaacc accatccgca
    73441 cgtcggagat gtttttctcc accacgcgca agccgttgag gatggcggca gtgctgataa
    73501 ttgccgtgcc gtgctgatcg tcgtggaata ccggaatatt catccgctcg cgcagtttct
    73561 gttcaatata gaaacattct ggcgctttaa tgtcttcgag gttgatgccg ccgaaggtcg
    73621 gttcgagcgc agcgacgact tcaataaatt tgtccgggtc gagttcatca acttcaatgt
    73681 caaatacatc aatcccggcg aatttcttaa acagaacgcc cttgccttcc atcaccggtt
    73741 tgcctgccag cgcgccaatg ttgcctaacc ccagcaccgc cgtaccgtta gagatcaccg
    73801 ctaccaggtt accgcgcgcg gtatatttgt aggcttttaa cgggtctttt tcgatttcaa
    73861 gacaaggtgc ggcaacgcct ggtgagtagg ccagcgccag atcgcgctgc gttgccagag
    73921 gcttggttgg agaaacctgg attttccctg gaactggaaa ttcatggaaa tcaagtgcac
    73981 tttgttttaa ctggtcatcc atttggttgt tcctttcacg taacgttcac aaataaagtg
    74041 tgtgggcaac agcccctgtc cacaacgtgg cgcacattat taccctgccg gagtctacag
    74101 actttgagca agtccaaact ctcaccatta atataatgtt ttggtaataa tcctataaca
    74161 ctgatgttac ctgcttaatc cagcaatacc atgcctgtct gctatgcttt tttgatgcgt
    74221 ttagcgaaat ttctcagaag tgtgaattaa cgcactcatc taacacttta cttttcaagg
    74281 agtatttcct atgaacgagt tagacggcat caaacagttc accactgtcg tggcagacag
    74341 cggcgatatt gagtccattc gccattatca tccccaggat gccaccacca atccttcgct
    74401 gttactcaag gctgccggat tatcacaata tgagcattta atagacgatg ctatcgcctg
    74461 gggtaaaaaa aatggcaaga cccaggaaca acaggtggtc gcagcgtgtg acaaactggc
    74521 ggtcaatttc ggtgctgaaa ttctcaaaat cgtacccggt cgcgtgtcaa cagaagttga
    74581 tgcacgcctc tcttttgata aagaaaagag tattgagaag gcgcgccatc tggtggactt
    74641 gtatcagcaa caaggcgttg agaaatcacg cattctgatc aagctggctt cgacctggga
    74701 aggaattcgc gcggcagaag agctggaaaa agaaggtatt aactgcaacc tgacgctgct
    74761 gttttctttt gcacaggcgc gggcctgtgc agaagcaggc gtttttctga tttcgccgtt
    74821 tgtcgggcgt atttatgact ggtatcaggc acgcaagccg atagacccgt atgtggtgga
    74881 agaagatcca ggcgttaaat cagtacgcaa tatctacgac tactataagc aacaccacta
    74941 tgaaaccatt gtgatgggcg cgagcttccg tcgcaccgaa caaatcctcg ccttaaccgg
    75001 ctgcgaccga ctgactatcg caccgaattt gctgaaggag ctacaggaaa aagtttcgcc
    75061 agtggtacgt aaattaatcc caccttctca gacgttccca cgcccagctc ccatgagcga
    75121 agcggaattc cgttgggagc acaatcagga tgcgatggcg gtagaaaaac tgtctgaagg
    75181 cattcgtctg ttcgccgttg atcaacgcaa actggaagat cttcttgccg ccaaactata
    75241 aaccagccac ggagtgttat atgtcccgaa aagaccttgc caatgcgatt cgcgcactca
    75301 gtatggatgc ggtacaaaaa gccaattctg gtcatcccgg cgcgccgatg ggcatggctg
    75361 atattgccga agtgctgtgg aacgattttc ttaaacataa ccccaccgac ccaacctggt
    75421 atgaccgtga ccgctttatt ctttccaacg gtcacgcgtc gatgctgctc tacagtttgc
    75481 tgcatctgac cggttacgac ctgccactgg aagaactgaa gaacttccgt cagttgcatt
    75541 cgaaaacccc tggtcacccg gaaatcggct atacgccagg tgttgaaacc accaccggtc
    75601 ctcttggaca aggtttggcg aacgccgtcg ggctggcgat agcagagcgt acgctggcgg
    75661 cgcagtttaa tcagccggat catgaaattg tcgatcactt cacctatgtg ttaatgggcg
    75721 acggctgcct gatggaaggt atctctcacg aagtctgttc gctggcgggc acgctggggc
    75781 tgggcaagct gattggtttt tacgatcaca acggtatttc catcgacggt gaaaccgaag
    75841 gctggtttac cgacgatacg gcaaaacgat ttgaagccta tcactggcat gtggtccacg
    75901 agatcgacgg ccacgatccg caggcggtga aggaagcgat ccttgaagcg caaagcgtga
    75961 aagataagcc gtcgctgatt atctgccgta cggtgattgg ctttggttcg ccgaataaag
    76021 caggtaagga agaggcgcac ggcgcaccac tgggggaaga agaagtggcg ctggcacggc
    76081 aaaaactggg ctggcaccat ccgccatttg agatccctaa agagatttat cacgcctgga
    76141 atgcccgcga aaaaggcgaa aaagcgcagc agcgctggaa tgagaagttt gccgcctata
    76201 aaaaggctca tccgcaactg gcagaagagt ttacccgtcg gatgagcggc ggtttgccga
    76261 aggactggga gaaaacgact cagaaatata tcaatgagtt gcaggcgaat ccggcgaaaa
    76321 tcgctacccg taaggcttcg caaaatacgc ttaacgctta cgggccgatg ctgcctgagt
    76381 tgctcggcgg ttcggcggat ctggctccca gcaacctgac catctggaaa ggttctgtgt
    76441 cgctgaagga agatccggcg ggcaactaca ttcactacgg ggtgcgtgaa tttggtatga
    76501 ccgctatcgc caacggcatc gcgcaccacg gcggctttgt gccgtatacc gcgacgttcc
    76561 tgatgtttgt tgaatacgcc cgtaacgccg cgcggatggc ggcactgatg aaagcgcggc
    76621 agattatggt ttatacccac gactcaattg gtctgggcga agatggtccg acgcaccagg
    76681 ctgttgagca actggctagt ctgcgcttaa cgccaaattt cagcacctgg cgaccgtgcg
    76741 atcaggtgga agcggcggtt ggctggaagc tggcggttga gcgccacaac ggaccgacag
    76801 cactgatcct ctcaaggcag aatctggcac aggtagaacg aacaccggat caggttaaag
    76861 agattgctcg tggcggttat gtgctgaaag acagcggcgg taagccagat attattctga
    76921 ttgccaccgg ttcagagatg gaaatcactc tgcaagcggc agagaaatta gcgggagaag
    76981 gtcgcaatgt tcgcgtggtt tccctgccct cgaccgatat tttcgatgcc caggatgagg
    77041 agtatcgcga gtcggtactg ccttcaaatg tttctgcccg cgtggcagta gaagcgggta
    77101 ttgccgatta ctggtacaaa tatgttggtc tgaaaggggc aattgtcggg atgacaggtt
    77161 atggggaatc tgctccggcg gataagctgt tcccgttctt tggctttacc gccgagaata
    77221 ttgtggcaaa agcgcataag gtactgggag tgaaaggtgc ctgatggtga tcgccggatg
    77281 ctgattgtcg gatgtgacgc tggtgcgtct tatccggtct acatgtcccc gccattttgt
    77341 ttaacgggtg atccacaacg tgggccaggc gtctggccca tgccagttat cgcaggtggg
    77401 ttctgcggcg taacgcacca ggcgaaaacg ctgaccgtca aagcgccagc gcgcctgaat
    77461 gccacaatcg cttaatccgc gcccttttgc taaggtcacc agttcacgcg atttttcatc
    77521 gaatgttgcg ttcatcagtt ccagttcatt ggtctcctga ccgttgttga acggcaaacg
    77581 caaccgtacc gggcgcgagg ccagcggctt tttacgcgac acaatccatg ccaaatcaat
    77641 ggtgttatag gcccctgcct cacagctaat catcatcagc gctttatcat cggtcagcgc
    77701 agtgacattc acctcacgac gtaacggatc aagcgaacag cgcagaccat tcatccgcca
    77761 gtttccataa tccagcaaat cgttacgttc ttcgcgtgag agtggcgtcg gcgttgggtt
    77821 aaccaccgcg acctctttca gcgcaggcgc tggcggtacg ctgagcggcg gttcgtcccc
    77881 tttctttatc cacgcggtct cactgccaac gcgcttttgc tgggcatcaa taaacaacaa
    77941 cgccgctttt aagccactca gagaaatagt ctgattgccg tcgcgtaagg tgatcgccct
    78001 cccttcctga atcatctgca aaaacgcggt gatggttgcc gtatcatcgg ttactaataa
    78061 ccatggtgaa atccgccact tgtcaccact taacgccaac ggctcaccat ctaacagcag
    78121 ccgtggcgct atctcccctt ctgacgcatc cggcgacttc aacccgccgc gctcaatacg
    78181 taaaacggca tcggtatgcg ccccggcgct gcggctcagg gtcatcacca gtccattatg
    78241 atcgcccgta ttgcgcgcta cacagaaatt ttgattatta caggtgacct gccagtcgga
    78301 aaacgcccgt tgcgctggtg ccgcccacac caaagacatc ggcaacaaag cgaaaaaaaa
    78361 gagaaggaaa atgcgatatc gcatggacgg tacgacccca gaagttcaaa caaacagcac
    78421 aagtcgtatc ttcgttgcgc aggtagcgcg gctcaatcgg atttgtcgga ttgagccaat
    78481 aataaacaac gaaatattat tgttattttt cagtccatta aatgtgacat ttgcaaatag
    78541 ttaagcaata acaccgtctt accgtcacgt atctcgccgg ttttaatcat ctccagcgcc
    78601 tggctgaacg gcagctcaag cacttcaata tcttcatctt cgacaccacc accggcgtta
    78661 gcgcgctggc tgtcactgta ttcggcgata aaaaagtgga ttagctcagt cacaccgcct
    78721 ggcgacatat acagttcaaa taatttgcgg acttcgccaa cttcatagcc cgtctcttca
    78781 atcgcttctt tgcgaatgca cacttccggt tcgtcgttat ccagcagccc ggcgcaggtt
    78841 tcaatcagtt gcccgctttc attgccatta acccaggtag cgacgcggaa ctggcgaatc
    78901 agaaccacgg tctttttctt cgcgttgtac aggaggatcg tcgcaccatt accgcgatcg
    78961 taaacttcac gtttatggcg gataacttcg ccatctttgc gcgtgagatc ataagtaatg
    79021 ttgtgcaggg taaaatagtt atcggagaga attttgtctt taacgagggt gatttgttgc
    79081 gtcataccga ctccacagcg cgaaatgaac aattatctta cgctgtgaag tcgggtttgt
    79141 ctgcgcaggc tatcagtgag atttgactga ttttacgccc agccagtcaa tgatcccctg
    79201 tgccgcatga cgtccttctg ccattgcggt aaccaccaga tccgcaccac gcacggcgtc
    79261 accaccggcg aagattttcg gattcgtggt ctggtaacgg tactggcttt ccacatccgc
    79321 gatgatgcgg ccccatttgt ctaccgttac accgtgcgac tccagccacg gcatcccgtg
    79381 cggattgaag ccaaacgcca taatcaccgc atcggctggc atgacaaatt cactgccttc
    79441 caccggcact ggacgccgac gtccctgggc gtccggctcg ccaagacgcg tgcgcaggaa
    79501 acgaatcccg cagacgtgac cttgttcatt cagttcaagc gccaccggct ggacgttaaa
    79561 ttcgaagttg gccccctctt cacgggcgtt cttcacctct ttcttcgagc ctggcatgtt
    79621 agcttcatca cgacgataag cgcaggtgac gttactcgcg ccgtggcgca gcgcggtacg
    79681 cacacagtcc atcgcggtgt cgccgccgcc cagtaccacg acgttaagtc cggcggtatt
    79741 gataaacggc tcttccggta gctcttcgag tcccatcacc tgtttagtgt tggcgatgag
    79801 gaacggcagc gcgtcataaa cgcccggcgc atcttcattg ggtaaacccg ctttcatgga
    79861 acggtaagtg cctacgccaa cgaagaccgc gtcgtattgt tccaaaagcg aattcaaaga
    79921 aacatcttta cccacttcac aattgagttc gaagtgaatc cccatcgcgc tgaagatttc
    79981 ccgacggcgt gccagcaggg atttatccag tttgaaagaa ggaatgccga aagtgagcaa
    80041 gccaccgatt tctggatggc gatcgtacac cgtcaccgcc acgccattgc gggtgagaac
    80101 atctgcacag gccagccctg ccggacccgc accgataatc gccacccgct tgtccacttt
    80161 ggtgacatgg cttaagtcag gacgccaacc tttcgccaac gcctgatctg aaatgtagcg
    80221 ttcaatgtta ccgatagtta ctgcgccgtg ctcatcgcga atagtacagg caccttcaca
    80281 caaacggtct tgcgggcaaa cgcgtccggt aatttccggc agggtgttgg tctggtgaga
    80341 aagctcgacg gcggcgtcga tgtttccggc tttcaccagt tcaatccact gcggtatatg
    80401 gttatgcagc gggcaggtcc attcacaaac gctgtgctcg ccgcacttaa ggcagcgcga
    80461 ggcttcccgt tgtgcctgat cggcacggaa tggcagataa atttcatcaa aaccggtttt
    80521 gcgcgcttca atcgccagtt tatctggctc gccacgcgcg ggcgttgcct gcatttgctc
    80581 gactttactc attaccggca tttcttgcgc cgcggtactg gcatgccacg gttgttgttc
    80641 ctgacgtgcg gtgcgcaagc ggcgggattt cgccatgccg gagagtgcgg cgtcagtgac
    80701 cagttgcagc gcgtccgccg ggcaattttc gacacaggca ggaccgtttt cgcgccctgc
    80761 acaaaggtcg catttatgcg ccgtggcttt tacttttcct accgcaacgg gtgtcagaac
    80821 gatttgcatc gtaccaaatg gacaggccac cacgcaggat ttacagccaa tacacttttg
    80881 ctgattgacc tgaatgctgt catcaacgtg gctgattgcg ccattagggc agctacgggc
    80941 gcagggcgca tcttcacaat ggtgacaggt cactgcacta cgttgctgct gatgtttgat
    81001 aaccgtaatt cggggatgaa aatggcgttg gctcaggaca tgttgctcgt cattgtgagc
    81061 catgacacag gcgatttcac aagcatgaca acccagacat tgctgactgt tggccataat
    81121 aaaacgattc ataacgacct tcttttttgg ttgtaaaaac cttattcttt atatgagtgt
    81181 tgttattacc cgacttacag gggaatcggc aatgttcatg tgcccagaat aagtaactat
    81241 ttcgctataa actgtggcag atcaaataat cccatcactg actaaattgt gtttcagcga
    81301 actggaacat taatgatttt ttgtggagaa gacgcgtgtg attgttaaac gacccgtctc
    81361 ggccagtctg gcccgggcct ttttttacat tgtgctgctg tcgattcttt ccacgggtat
    81421 cgctctgcta acgctggcga gcagtttacg cgacgctgag gctatcaata ttgccggatc
    81481 gctgcgtatg cagagttacc gcctgggcta cgacttgcaa agtggcagtc cacaactcaa
    81541 tgcacatcgc cagttatttc agcaggcact gcattcaccg gtattaacca atctcaacgt
    81601 ctggtatgtg ccagaggcag taaaaacccg ctatgcgcat ctgaatgcca actggctgga
    81661 gatgaataat cggctcagca agggcgactt gccgtggtat caggccaata ttaataatta
    81721 tgttaatcag atagacctgt tcgtgctggc tttacagcac tacgctgaac gcaaaatgct
    81781 gctggtggtg gcaatttccc tggctggcgg catcggtatt ttcacgctgg tcttttttac
    81841 tttgcgccgc atacgccatc aggtggttgc cccgctgaat cagctggtta ccgccagtca
    81901 gcgtattgaa cacgggcagt tcgactcgcc gccgctggat accagcctgc cgaatgagct
    81961 tggcctgctt gcaaaaacct ttaaccagat gtcgagcgag ctgcataaat tgtaccgttc
    82021 gctggaggcg tcagtagaag aaaagacccg cgatctccac gaggccaagc gtcgtctgga
    82081 ggtgttgtat cagtgttcgc aggcgctaaa caccagccag attgatgtgc attgtttccg
    82141 ccatattttg cagattgttc gcgacaatga agcggctgaa tatctggagt taaatgtcgg
    82201 tgacaactgg cggattagcg aagggcagcc aaacccggaa ttgccgatgc agattttacc
    82261 ggtgacaatg caagagacgg tttacggcga actgcactgg caaaatagtc acgtttcatc
    82321 atcagaaccg ctgcttaaca gcgtttcgtc gatgctggga cgcggtttgt actttaatca
    82381 ggcgcagaag cattttcagc agttattgtt gatggaagaa cgtgcgacca tcgcccgcga
    82441 attgcacgac tcgctggctc aggtactttc ttacttacgt atccagttga cgttactgaa
    82501 gcgttcgata ccggaagata acgccaccgc acaaagtatc atggccgatt tttcccaggc
    82561 gttgaatgat gcttatcggc agttacgcga gctgttgact actttccgcc tgacgctgca
    82621 gcaggcggat ctcccctccg cgttgaggga aatgctggat acgttacaaa atcaaaccag
    82681 cgccaaactg accctcgact gccgtctgcc aaccctggcg ctggatgcgc aaatgcaggt
    82741 gcatttgttg caaattattc gcgaagcggt gctgaatgcg atgaagcacg ccaacgccag
    82801 cgaaatcgcc gttagctgcg tcaccgcgcc ggatggcaat cacacagtct atattcgcga
    82861 caacgggatt ggcatcggtg aaccgaaaga acccgaaggc cattatggtc tgaatatcat
    82921 gcgcgaacgc gcggaaagat tgggtgggac gctgactttt tcacaacctt ccggcggcgg
    82981 cacgttagtg agtattagct ttcgctctgc ggagggtgag gaaagtcagc ttatttggca
    83041 tccatgctcg aaataagccg actttaaata aggctctaca acacaagcct gatacattta
    83101 cttacccgac ggcgaaacag caccgggtaa gtcctctgta ttttaccaac tgtaacgcaa
    83161 gttcagactg ccattaagat cgttgtagtt atggcttccc gtctcaccaa caacctggcc
    83221 agtaatactc cactggctgt ccaggttcac ctgtatccct actttcgcct gcacccggtt
    83281 agacgggata ccctgtttcg tctcagcgtc atcaaatgac acagcaacat caccgctggt
    83341 gtacaaccag ttcacttcag caaacggctg aataatggag gttttattct tgtgcagttt
    83401 gccatcaaca cgcagcccta accgggtatt ccagttgtcg ctgtcctggc cgctgatgcg
    83461 ggtaccatta ttgtccgtaa cactgtcggc gctgtaacgc tgccagacga cctgtgcctg
    83521 tggcgtcagg ctgagcgtgt gttgattttc cagtgcaata tcgtaacggt agccgccttc
    83581 cagtgatgct gcctgtgccg tagaatcata gctttctgac ccctcactgc cattttgcac
    83641 actgttgtta tagaagccat actgatacca gctgtctaca tacaacccgc gatgatgttg
    83701 cgcatcggca aaccatgtgg catatatgcc gaggttgtag ccaccgacgt taccgcttgc
    83761 cgagaattgg ctaccgtccg cacccctgtt gctggtactg tcggtgtcgg cattgacgta
    83821 actcgccatc cccccggctt tcagactctg cacaccgttg ccccacgtca gaatgtcgcc
    83881 gcctagttgg atttgcgaat agttgttgtt gatatcaaca ttgccgtcag ccgcctgaga
    83941 cgctgcattc cccgccttaa aacgcatcca gacactgcca tcttcattat ggtactgact
    84001 accttcgcgg tcgtacaacg tctgcatttg caggttacgc accatccact gattgccgag
    84061 gtaagcgccg atatcagggc gatattgcgg cgtcaccggg gtatcatctg tgccactaca
    84121 atcaccatca tccggttcag gcggaacgct ggattgcgaa cgtaaatacc agtcgccacc
    84181 gtcttcatag agacgatatt cgtacaatcc aatctgcacc cgatttccct gctcaaatgt
    84241 tccggatgac tgaccgccaa cgtcgatcac ttctatacca tttttcgtct gtgcaccttt
    84301 accattgacg ttagcaacat agaccatcgt gttaccagaa gtatcgccag agatgaccag
    84361 tttatcggtt ggagaactgt catcacccag ttgactgttg atgttaatct ggcttccgtt
    84421 atcgccgtga tagttgccgt tgatggtcag tgtgttgccc gtttcacctc catggctcat
    84481 ggcaatattg ccactgttgt agacatcgcc attcaccacg ccgctgccgc cgaaggtagc
    84541 tccctccgca acattcaccg cctgggtact gccagatgca ccaatcaccc cgctgtccgt
    84601 cagccacaac gttccatcat tcacacgggt atccccgctc caggtgttgg caacattaag
    84661 ggtaagtgtg ccgtcgcccg ctttagtcag ggttttgcca tcccagccag tgacgctatt
    84721 cgggttaaca tcgaccagtt cggtattgag ggtaaagttc tcattcgggt cgctgagagt
    84781 gaaggtgccg tgggcatcgg tagcggcaag tttttcatcc gcataccagg acagtgaagt
    84841 ggtcagttcg tactgtgtgt catccgcaga gttaacatga ctgtccaccg tcaggaagtc
    84901 gacttttctt gcctccattc cggcgacggt caacgtatca aaatcgccgt tgatggcgtt
    84961 atcagcatca atcagagtaa atgcataagg atcgtgggtc agcgggttgt taatgttacc
    85021 aatacccgta atgttgagcg tgccacccag cgaggcgtca tgagcggtaa tgatcggact
    85081 actggcatcc gtcccaagat tgatactcag gacactgccg tcctgctgat ccagcatgcc
    85141 agtagcaagt gttgcaccgg cagccagtga agtggtaccg ccatcaaatg tggtgatatt
    85201 ggtcagcgta tactgccccg cagcattcag attcagcgta gcgtagtttg ccacatcacc
    85261 ggtccccagc gcgccagtat gatcagcatt gagcgtaccc gcttcaatgt ccgtgccgcc
    85321 gctgtaggtg ttggcatgag caaccgtaat ggtgttggca tcttctttta ccaccttacc
    85381 cacataactg cctactagca tgtcatcgtc atatttatcg tagtcgctaa tcacattacc
    85441 actgttcagc atccagtcct ctgcaccgcc cagcgtcagg gttccgccgt tgataatatt
    85501 acccgccccc agcgctgatg catccgccac agccagatcc gcatccatat ccttattcgc
    85561 accaataatc caggtaccat taaagctgtc attgtcaccg ctgacggtaa gatcccacac
    85621 ggtactgact tccccgctac cactgaggct gttgctcagg cctcccccag ctggcgaaaa
    85681 aatactcagc agcccgtcaa ccacgatatc cccgcggcca aggttggtcg tctcgctaat
    85741 tacctgggcg ccttgttcta tgttgatggt tgcgttcaga ccactgagat cgctggtcga
    85801 tctaatattg agttcactgt ataaattcag caaaccactg ccggtaacga tcccctcaaa
    85861 aaaggagacc catctatatc gagaaccatg tccgatcgtc aactcaccac cgtccagatc
    85921 aagcatactg cccacaccca cagatacaga atcaaagctc tgactctcac cgttcagaat
    85981 gtaagcattg ccactttcca gttccagcac attatccact tgagctttca ggctcgcccc
    86041 cttatcgagg gggctgccgc tgtaggcaga gatcgccatg ccgctactgt aactatgttg
    86101 tctgtcagaa gcgtctaccg tttccccggt aaccattgca ttcgtcacct cagatgtgga
    86161 ttccgcctgc gcactgagcg ggcttaacgc caggagcacc atcgctgcca ccgaccgcac
    86221 ctgggttttc atccgactgt cgccgcgcga gagttcggaa gcaacgacgt acatattacg
    86281 cgcagcattc cagacaatgt tgtaagttct gttcatgaat tagtccttta agattaagag
    86341 cgcgtgttcc tcgctcaggc tgtgatgcaa ttgatgaata atgcggatga cctgctgacc
    86401 ggtgatcagc cgggagcact catactgccg ctcagtacct ttgtgcctgg gacattgcag
    86461 aaaatcctgg tgatcaaaat cgatgcgcat atcgtcccag caaccatggc agccataaga
    86521 attgaatacg cgccacggtg tatagaactc acttagagga ttggtaaacc cactaatcaa
    86581 caccaccggg atgtgacaac tccacgctaa ccaactcaag ccgctgctca ggccaacaaa
    86641 aaaggccgca tggcgaagca aatccacccg ttgctgtagc ggcagcgcgc cagtgaaatc
    86701 ctccgcgccg ttcgggatgt gattccagac aaaaccataa ccatggtgcg cctctttatc
    86761 gatgcacagc actcggtagc ccagcgtttt cagttcagaa attacctgcg accagccata
    86821 gccgttgttc cagtatttcg cctggcagga agactgaaca gcgatacaaa cgtagggttc
    86881 gtcaatgctg cgcacagcat caagtttcag taacggcggc tcttcatgcg gcaaaattcc
    86941 caggatataa gcagcgatac gactaaagcc gacctggcgg aaatccaccg gttgatgagt
    87001 atcattgcct ttgaaaaaca gtccgatgcg gtagctggcg taggggctgt gggtttgaat
    87061 gctttcacga gtggaaaaag tgatttgcgg atactggtcg cgtagcagtt caatgatttc
    87121 ctctgtcatc acacattcca actggcactg atgcttctgg cggaaacgtt cagcataggg
    87181 aaaccaacca aggagctcct ccaaagtgcc gatgggaaag tggataatga cagttttatc
    87241 acgcagatcc tgaactacgt ccagcacagg tgtcgtttca ccgtctttaa atacctggat
    87301 gcgaaagttg atgaagtact ttttattact catcacacag ccttgatcag tctcacaggt
    87361 cagtaatata ttgccggagt cgtcatcaat taaatttacc tgccactgac cagggccaag
    87421 tttaacgcgg gctccgtcat taaaatcaaa acgaatacca tgcaacgctt tttgggtagg
    87481 aataaaaggg cgtgtagaga acaacagagg tgtttgtccg tctattatgt ttggcagtgt
    87541 gtcattatta ctcactgtaa agctcctttt tatcgaatta gtaaggcatc atgttaggcg
    87601 ataaactaat at
//
LOCUS       DAZFJL010000003        72273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFJL010000003 DAZFJL010000000
VERSION     DAZFJL010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 72273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.003"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..356)
                     /locus_tag="ACHJ8C_000188"
     CDS             complement(<1..356)
                     /locus_tag="ACHJ8C_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFO5550111.1"
                     /translation="MLIGQALDLISRYDSLRNPLTSLGDYLAPELISRCLAESGTVTL
                     HKRRLPLEMMVWCIVGMALERKEPLHQIANRLDIMLPGNRPFVAPSAVIQTRQRLGIE
                     AVRRVFTKTAQLWHNAT"
     gene            complement(482..3157)
                     /gene="adhE"
                     /locus_tag="ACHJ8C_000189"
                     /gene_synonym="adhC"
     CDS             complement(482..3157)
                     /gene="adhE"
                     /locus_tag="ACHJ8C_000189"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO5550112.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDTTGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            3550..3603
                     /gene="ychE"
                     /locus_tag="ACHJ8C_000190"
     CDS             3550..3603
                     /gene="ychE"
                     /locus_tag="ACHJ8C_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO5550113.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            3634..4281
                     /gene="ychE"
                     /locus_tag="ACHJ8C_000191"
     CDS             3634..4281
                     /gene="ychE"
                     /locus_tag="ACHJ8C_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO5550114.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            5019..6650
                     /gene="oppA"
                     /locus_tag="ACHJ8C_000192"
     CDS             5019..6650
                     /gene="oppA"
                     /locus_tag="ACHJ8C_000192"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO5550115.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            6736..7656
                     /gene="oppB"
                     /locus_tag="ACHJ8C_000193"
     CDS             6736..7656
                     /gene="oppB"
                     /locus_tag="ACHJ8C_000193"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO5550116.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            7671..8579
                     /gene="oppC"
                     /locus_tag="ACHJ8C_000194"
     CDS             7671..8579
                     /gene="oppC"
                     /locus_tag="ACHJ8C_000194"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO5550117.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            8591..9604
                     /gene="oppD"
                     /locus_tag="ACHJ8C_000195"
     CDS             8591..9604
                     /gene="oppD"
                     /locus_tag="ACHJ8C_000195"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO5550118.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGKQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMDICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            9601..10605
                     /gene="oppF"
                     /locus_tag="ACHJ8C_000196"
     CDS             9601..10605
                     /gene="oppF"
                     /locus_tag="ACHJ8C_000196"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO5550119.1"
                     /translation="MNAETEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10658..10987)
                     /gene="yciU"
                     /locus_tag="ACHJ8C_000197"
     CDS             complement(10658..10987)
                     /gene="yciU"
                     /locus_tag="ACHJ8C_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO5550120.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(11022..12482)
                     /gene="cls"
                     /locus_tag="ACHJ8C_000198"
     CDS             complement(11022..12482)
                     /gene="cls"
                     /locus_tag="ACHJ8C_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO5550121.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            12625..12798
                     /gene="yciY"
                     /locus_tag="ACHJ8C_000199"
     CDS             12625..12798
                     /gene="yciY"
                     /locus_tag="ACHJ8C_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO5550122.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(12853..14106)
                     /gene="kch"
                     /locus_tag="ACHJ8C_000200"
     CDS             complement(12853..14106)
                     /gene="kch"
                     /locus_tag="ACHJ8C_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO5550123.1"
                     /translation="MSHWTTFKQTATKLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(14406..14702)
                     /gene="yciI"
                     /locus_tag="ACHJ8C_000201"
     CDS             complement(14406..14702)
                     /gene="yciI"
                     /locus_tag="ACHJ8C_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO5550124.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            14926..15642
                     /gene="tonB"
                     /locus_tag="ACHJ8C_000202"
     CDS             14926..15642
                     /gene="tonB"
                     /locus_tag="ACHJ8C_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO5550125.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQP
                     QYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGK
                     PGSGIVVNILFKINGTTEIQ"
     gene            complement(15682..16080)
                     /gene="yciA"
                     /locus_tag="ACHJ8C_000203"
     CDS             complement(15682..16080)
                     /gene="yciA"
                     /locus_tag="ACHJ8C_000203"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO5550126.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCIQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPIE"
     gene            complement(16186..16725)
                     /gene="yciB"
                     /locus_tag="ACHJ8C_000204"
     CDS             complement(16186..16725)
                     /gene="yciB"
                     /locus_tag="ACHJ8C_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO5550127.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQSVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(16755..17498)
                     /gene="yciC"
                     /locus_tag="ACHJ8C_000205"
     CDS             complement(16755..17498)
                     /gene="yciC"
                     /locus_tag="ACHJ8C_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO5550128.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(17526..17615)
                     /locus_tag="ACHJ8C_000206"
                     /pseudo
     CDS             complement(17526..17615)
                     /locus_tag="ACHJ8C_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            17854..18507
                     /gene="ompW"
                     /locus_tag="ACHJ8C_000207"
     CDS             17854..18507
                     /gene="ompW"
                     /locus_tag="ACHJ8C_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO5550129.1"
                     /translation="MKKLTVAALAVATLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAASPFRHKVGTATTGDIATVHHLPP
                     TLMAQWYFGDSGSKFRPYIGAGINYTTFFNNDFNDTGEGLGLSDLSLKDSWGVAGQVG
                     LDYLINRDWLLNMSVWYMDIDTEVKYKSNGAVLPAGRYSDNVRLDPWVFMFSAGYRF"
     gene            complement(18558..19364)
                     /gene="trpA"
                     /locus_tag="ACHJ8C_000208"
     CDS             complement(18558..19364)
                     /gene="trpA"
                     /locus_tag="ACHJ8C_000208"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO5550130.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEVLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAECEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            complement(19364..20557)
                     /gene="trpB"
                     /locus_tag="ACHJ8C_000209"
     CDS             complement(19364..20557)
                     /gene="trpB"
                     /locus_tag="ACHJ8C_000209"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO5550131.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETDVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(20569..21927)
                     /gene="trpCF"
                     /locus_tag="ACHJ8C_000210"
     CDS             complement(20569..21927)
                     /gene="trpCF"
                     /locus_tag="ACHJ8C_000210"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO5550132.1"
                     /translation="MQTVLAKIVADKAIWVEARKQQQPLASFQNEIQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLPI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLNDEQYRQLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLNATVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPARDFQHIDKYVFDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            complement(21931..23526)
                     /gene="trpD"
                     /locus_tag="ACHJ8C_000211"
     CDS             complement(21931..23526)
                     /gene="trpD"
                     /locus_tag="ACHJ8C_000211"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO5550133.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(23526..25088)
                     /gene="trpE"
                     /locus_tag="ACHJ8C_000212"
     CDS             complement(23526..25088)
                     /gene="trpE"
                     /locus_tag="ACHJ8C_000212"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO5550134.1"
                     /translation="MQTQKPTLELLTCKGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALSDTVTIQALSGNGEALLTLLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFEN
                     LPQLSAENSCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(25110..25203)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(25180..25224)
                     /gene="trpL"
                     /locus_tag="ACHJ8C_000213"
     CDS             complement(25180..25224)
                     /gene="trpL"
                     /locus_tag="ACHJ8C_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO5550135.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            25362..26243
                     /gene="rnm"
                     /locus_tag="ACHJ8C_000214"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             25362..26243
                     /gene="rnm"
                     /locus_tag="ACHJ8C_000214"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO5550136.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQSIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            26240..26860
                     /gene="yciO"
                     /locus_tag="ACHJ8C_000215"
     CDS             26240..26860
                     /gene="yciO"
                     /locus_tag="ACHJ8C_000215"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO5550137.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            26888..28783
                     /gene="yciQ"
                     /locus_tag="ACHJ8C_000216"
     CDS             26888..28783
                     /gene="yciQ"
                     /locus_tag="ACHJ8C_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFO5550138.1"
                     /translation="MAGIYRCILLLIVGLFFSSLSYAKNTEIPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPNGDAALLNYRIVGVTRDGVPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTLFSLKLPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLGNAPAPQTTNIFSHLLLPSTSSLLICFPSLFLACGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDHYDKNQQILIRV
                     NEGATRNNAVILPAEQLLLEALFRKGDKVVLTGRRNRVLRKAFLRMQKFYLPRKKSSF
                     YRPDAFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMICWIIPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAVSAN
                     LGREWAARITPSLTAAVRAPEIARSGVLFSLQTHLSCGAYTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            28996..29871
                     /gene="rluB"
                     /locus_tag="ACHJ8C_000217"
     CDS             28996..29871
                     /gene="rluB"
                     /locus_tag="ACHJ8C_000217"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO5550139.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
     gene            complement(29911..30501)
                     /gene="cobO"
                     /locus_tag="ACHJ8C_000218"
     CDS             complement(29911..30501)
                     /gene="cobO"
                     /locus_tag="ACHJ8C_000218"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO5550140.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTSA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(30498..31256)
                     /gene="yciK"
                     /locus_tag="ACHJ8C_000219"
     CDS             complement(30498..31256)
                     /gene="yciK"
                     /locus_tag="ACHJ8C_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO5550141.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEEIGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQDPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            31476..32525
                     /gene="sohB"
                     /locus_tag="ACHJ8C_000220"
     CDS             31476..32525
                     /gene="sohB"
                     /locus_tag="ACHJ8C_000220"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO5550142.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDTHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINSSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(32561..32812)
                     /gene="yciN"
                     /locus_tag="ACHJ8C_000221"
     CDS             complement(32561..32812)
                     /gene="yciN"
                     /locus_tag="ACHJ8C_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO5550143.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            33192..35789
                     /gene="topA"
                     /locus_tag="ACHJ8C_000222"
     CDS             33192..35789
                     /gene="topA"
                     /locus_tag="ACHJ8C_000222"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO5550144.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSTA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            35999..36973
                     /gene="cysB"
                     /locus_tag="ACHJ8C_000223"
     CDS             35999..36973
                     /gene="cysB"
                     /locus_tag="ACHJ8C_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO5550145.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            37292..37432
                     /gene="ymiA"
                     /locus_tag="ACHJ8C_000224"
     CDS             37292..37432
                     /gene="ymiA"
                     /locus_tag="ACHJ8C_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO5550146.1"
                     /translation="MRLAMPSGNQEPRRNPELKRKAWLAVFLGSALFWVVVALLIWKL
                     WG"
     gene            37435..37602
                     /gene="yciX"
                     /locus_tag="ACHJ8C_000225"
     CDS             37435..37602
                     /gene="yciX"
                     /locus_tag="ACHJ8C_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO5550147.1"
                     /translation="MVGQEQLESSPLCQHSDNEPEAKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            37716..37811
                     /gene="ymiC"
                     /locus_tag="ACHJ8C_000226"
     CDS             37716..37811
                     /gene="ymiC"
                     /locus_tag="ACHJ8C_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO5550148.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            37961..40636
                     /gene="acnA"
                     /locus_tag="ACHJ8C_000227"
     CDS             37961..40636
                     /gene="acnA"
                     /locus_tag="ACHJ8C_000227"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO5550149.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGKSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSVLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASRELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVDQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAIRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLELTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(40700..41290)
                     /gene="ribA"
                     /locus_tag="ACHJ8C_000228"
     CDS             complement(40700..41290)
                     /gene="ribA"
                     /locus_tag="ACHJ8C_000228"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO5550150.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIIERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            41460..42224
                     /gene="pgpB"
                     /locus_tag="ACHJ8C_000229"
     CDS             41460..42224
                     /gene="pgpB"
                     /locus_tag="ACHJ8C_000229"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO5550151.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWLSGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAIMLFAILAAAILMGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATMI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            42373..42681
                     /gene="lapA"
                     /locus_tag="ACHJ8C_000230"
     CDS             42373..42681
                     /gene="lapA"
                     /locus_tag="ACHJ8C_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO5550152.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSASKE
                     "
     gene            42688..43857
                     /gene="lapB"
                     /locus_tag="ACHJ8C_000231"
     CDS             42688..43857
                     /gene="lapB"
                     /locus_tag="ACHJ8C_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO5550153.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            44049..44786
                     /gene="pyrF"
                     /locus_tag="ACHJ8C_000232"
     CDS             44049..44786
                     /gene="pyrF"
                     /locus_tag="ACHJ8C_000232"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO5550154.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAAMAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVSELQQRGFDIFLDLKFHDIPNTAAHAVAVAADLGVWMVNVHASG
                     GARMMAAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            44786..45112
                     /gene="yciH"
                     /locus_tag="ACHJ8C_000233"
     CDS             44786..45112
                     /gene="yciH"
                     /locus_tag="ACHJ8C_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO5550155.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPARPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(45238..45456)
                     /gene="osmB"
                     /locus_tag="ACHJ8C_000234"
     CDS             complement(45238..45456)
                     /gene="osmB"
                     /locus_tag="ACHJ8C_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO5550156.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(45725..46474)
                     /gene="yciT"
                     /locus_tag="ACHJ8C_000235"
     CDS             complement(45725..46474)
                     /gene="yciT"
                     /locus_tag="ACHJ8C_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO5550157.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEQSKLTVHVVDI"
     gene            complement(46564..46737)
                     /gene="yciZ"
                     /locus_tag="ACHJ8C_000236"
     CDS             complement(46564..46737)
                     /gene="yciZ"
                     /locus_tag="ACHJ8C_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO5550158.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            46775..46903
                     /gene="ymiD"
                     /locus_tag="ACHJ8C_000237"
     CDS             46775..46903
                     /gene="ymiD"
                     /locus_tag="ACHJ8C_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO5550159.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYVRFR"
     gene            complement(46885..48870)
                     /gene="pdeR"
                     /locus_tag="ACHJ8C_000238"
     CDS             complement(46885..48870)
                     /gene="pdeR"
                     /locus_tag="ACHJ8C_000238"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO5550160.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTEITEPDRIL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWGGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEALA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSRLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRT"
     gene            48924..49027
                     /locus_tag="ACHJ8C_000239"
                     /pseudo
     CDS             48924..49027
                     /locus_tag="ACHJ8C_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(49104..51038)
                     /gene="rnb"
                     /locus_tag="ACHJ8C_000240"
     CDS             complement(49104..51038)
                     /gene="rnb"
                     /locus_tag="ACHJ8C_000240"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO5550161.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DGKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSTDGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(51106..52233)
                     /gene="yciW"
                     /locus_tag="ACHJ8C_000241"
     CDS             complement(51106..52233)
                     /gene="yciW"
                     /locus_tag="ACHJ8C_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO5550162.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARLLAVELFPDPLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTACDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEYTKSSTAELCQLTETLSLHPISLSLLEMLLNSTRVKTQPGNLLAALLCARIN
                     GSPACFAACMNSSNEYKKISLLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            complement(52377..53165)
                     /gene="fabI"
                     /locus_tag="ACHJ8C_000242"
     CDS             complement(52377..53165)
                     /gene="fabI"
                     /locus_tag="ACHJ8C_000242"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO5550163.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            53643..54209
                     /locus_tag="ACHJ8C_000243"
     CDS             53643..54209
                     /locus_tag="ACHJ8C_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO5550164.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            54358..55479
                     /locus_tag="ACHJ8C_000244"
     CDS             54358..55479
                     /locus_tag="ACHJ8C_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO5550165.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            55479..58586
                     /locus_tag="ACHJ8C_000245"
     CDS             55479..58586
                     /locus_tag="ACHJ8C_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFO5550166.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPNTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYVVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     PRRQE"
     gene            58590..59963
                     /locus_tag="ACHJ8C_000246"
     CDS             58590..59963
                     /locus_tag="ACHJ8C_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFO5550167.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFAPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQSQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            59972..61135
                     /locus_tag="ACHJ8C_000247"
     CDS             59972..61135
                     /locus_tag="ACHJ8C_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFO5550168.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINTMTLLR
                     GGLIAAILCALLTVLCAWVQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(61183..61989)
                     /gene="sapF"
                     /locus_tag="ACHJ8C_000248"
     CDS             complement(61183..61989)
                     /gene="sapF"
                     /locus_tag="ACHJ8C_000248"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO5550169.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(61991..62983)
                     /gene="sapD"
                     /locus_tag="ACHJ8C_000249"
     CDS             complement(61991..62983)
                     /gene="sapD"
                     /locus_tag="ACHJ8C_000249"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO5550170.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(62983..63873)
                     /gene="sapC"
                     /locus_tag="ACHJ8C_000250"
     CDS             complement(62983..63873)
                     /gene="sapC"
                     /locus_tag="ACHJ8C_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO5550171.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(63860..64825)
                     /gene="sapB"
                     /locus_tag="ACHJ8C_000251"
     CDS             complement(63860..64825)
                     /gene="sapB"
                     /locus_tag="ACHJ8C_000251"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO5550172.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIISAIRHMVLPVLTLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(64822..66465)
                     /gene="sapA"
                     /locus_tag="ACHJ8C_000252"
     CDS             complement(64822..66465)
                     /gene="sapA"
                     /locus_tag="ACHJ8C_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO5550173.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYAGKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(66778..67023)
                     /gene="ymjA"
                     /locus_tag="ACHJ8C_000253"
     CDS             complement(66778..67023)
                     /gene="ymjA"
                     /locus_tag="ACHJ8C_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HFO5550174.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(67157..67264)
                     /locus_tag="ACHJ8C_000254"
                     /pseudo
     CDS             complement(67157..67264)
                     /locus_tag="ACHJ8C_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001614509.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(67450..68427)
                     /gene="pspF"
                     /locus_tag="ACHJ8C_000255"
     CDS             complement(67450..68427)
                     /gene="pspF"
                     /locus_tag="ACHJ8C_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO5550175.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERKSDIMLMAEHFAIQMCREIKLPLFPGFTEHARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPSEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            68594..69262
                     /gene="pspA"
                     /locus_tag="ACHJ8C_000256"
     CDS             68594..69262
                     /gene="pspA"
                     /locus_tag="ACHJ8C_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO5550176.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKEQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            69316..69540
                     /gene="pspB"
                     /locus_tag="ACHJ8C_000257"
     CDS             69316..69540
                     /gene="pspB"
                     /locus_tag="ACHJ8C_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO5550177.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRYGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            69540..69899
                     /gene="pspC"
                     /locus_tag="ACHJ8C_000258"
     CDS             69540..69899
                     /gene="pspC"
                     /locus_tag="ACHJ8C_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO5550178.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPFSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            69908..70129
                     /gene="pspD"
                     /locus_tag="ACHJ8C_000259"
     CDS             69908..70129
                     /gene="pspD"
                     /locus_tag="ACHJ8C_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO5550179.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            70204..70518
                     /gene="pspE"
                     /locus_tag="ACHJ8C_000260"
     CDS             70204..70518
                     /gene="pspE"
                     /locus_tag="ACHJ8C_000260"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO5550180.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKESLSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            70728..>72273
                     /gene="ycjM"
                     /locus_tag="ACHJ8C_000261"
     CDS             70728..>72273
                     /gene="ycjM"
                     /locus_tag="ACHJ8C_000261"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HFO5550181.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYPQWLQRIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFIAVDP
                     KTDLSAVIRPRALPLLTPFQMRDHSMRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGEGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPENEILALVEVLQQEGALVNWKNNPDGTRSPYEMNVTYMDALS
                     RRECSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     SSKEITTELNNKATLRYSIYYKLSHLITLRRSHKEFHPDNNFTIDAINSFVMRIQRSN
                     ADGDCLT"
BASE COUNT        18238 a        17438 c        18620 g        17977 t
ORIGIN      
        1 gtggcgttat gccagagctg cgctgttttc gtgaacacgc ggcggacagc ctcaattccc
       61 agacgctggc gggtctgaat aacggcactg ggggcaacga aggggcgatt gcccggcagc
      121 atgatgtcca ggcgattcgc aatctggtga agaggttctt tacgctcaag cgccatgcca
      181 acaatacacc agaccatcat ttcgagggga agacggcgct tgtgtagcgt tacagtacct
      241 gattcggcaa ggcaacgaga gatgagttcg ggggcgaggt aatcccccag agaagtcagt
      301 gggttacgca gagaatcgta acgggatatc aaatcaaggg cctgtccaat gagcataaaa
      361 aaatccggaa acgagtgagc gtttccggat tcttacacag ccactggatc ggtcaactga
      421 tccttaactg actggcatta ccccttatgg ggccgtttat gttgccagac agcgctactg
      481 attaagcgga ttttttcgct tttttctcag ctttagccgg agcagcttct ttcttcgctg
      541 cagtttcgcc ttctacataa tcacgaccgt agtaggtatc cagcagaatc tgtttcagct
      601 cggagatcag cgggtaacgc gggttagcgc cggtgcactg gtcatcgaat gcatcttcag
      661 acagtttatc cacgttcgcc aggaagtctg cttcctgaac gccagcttca cggatagatt
      721 tcggaatacc cagttcagct ttcagcgttt ccagccatgc cagcagtttc tcgatcttag
      781 ctgcagtacg gtcgcccggt gcgctcagac ccaggtggtc agcaatttca gcataacgac
      841 ggcgagcctg cggacggtca tactggctga atgcagtctg cttggtcggg ttgtcattcg
      901 cgttgtagcg aataacgtta caaatcagca gggcgtttgc cagaccgtgc ggaatatgga
      961 actgggaacc cagtttgtgc gccattgagt gacatacacc caggaaggcg ttcgcaaacg
     1021 cgatacccgc gatagtcgct gcactgtgaa cacgttcacg cgctaccgga tttttagacc
     1081 cttcgtggta ggacgctggc agatattctt tcagtagttt cagtgcctgc agagcctgac
     1141 catcagagaa ctcagatgcc agtacagaaa cataagcttc catggcgtga gttactgcgt
     1201 ccagaccacc gaaagcacac agggacttcg gcatgtccat aaccaggttg gcgtcgacaa
     1261 tcgccatatc cggggtcagt gcatagtctg ccagcggata tttctgacca gtagtgtcgt
     1321 cagttacaac cgcaaacgga gtgacttcag aacctgtacc agaagtggtg gtgacagcga
     1381 tcattttcgc tttcacaccc attttcggga acttgtagat acgtttacgg atatccataa
     1441 agcgcagcgc cagttcttcg aagtgagttt ccggatgttc gtacataacc cacatgatct
     1501 tcgcagcgtc catcggggaa ccaccaccca gcgcgataat cacgtctggt ttgaaggagt
     1561 ttgccagttc tgcaccttta cgaacgatac tcagggtcgg gtcagcttct acttcgaaga
     1621 agacttcagt ttcaacgcct gctgctttca atacggaagt gatctgatca gcatagccat
     1681 tgttgaacag gaagcggtca gtcacgatga gcgcacgttt gtggccatca gtaatcactt
     1741 catccagcgc gattggcagg gagccacggc ggaagtagat agatttcgga agtttgtgcc
     1801 acaacatgtt ttcagctcgc ttagcaacgg ttttcttgtt gatcaggtgt ttcggaccaa
     1861 cgttttcaga gatggagtta ccaccccagg aaccacaacc cagagtcagg gaaggtgcga
     1921 gtttgaagtt atacaggtca ccgataccac cctgagaagc cggggtgtta atcaggatac
     1981 gagccgtttt cattttctga ccgaagtaag aaacgcgagc cggttggtta tcctggtcag
     2041 tgtacaggca agaggtatga ccgataccgc ccatagcaac cagtttctct gctttttcta
     2101 ccgcatcttc gaaatcttta gcacggtaca ttgccagagt cggggacagt ttttcatgtg
     2161 cgaacggttc gctttcatca acaacggtca cttcaccgat cagaatcttg gtgttttctg
     2221 gtacagagaa gcctgccagt tcagcaattt tataggctgg ctgaccaacg atagccgcgt
     2281 tcagcgcacc gtttttcagg ataacgtcct gaacagcttt cagctcttta ccctgcaaca
     2341 gatagccgcc gtgggttgcg aaacgttcac gtactgcgtc ataaacagag tcaacaacaa
     2401 caacagactg ttcagaagca cagattacac cattgtcgaa ggttttggac atcagtacag
     2461 atgcaactgc acgtttgata tcagcagttt catcgataac aaccggagtg ttgcccgcgc
     2521 ctacgccgat agctggttta ccggagctgt atgcggcttt aaccatgccc ggaccaccag
     2581 tcgcgaggat caggttgatg tctgggtggt gcatcagtgc gttagacagc tcaacagaag
     2641 gttgatcgat ccagccgatc agatctttcg gagcaccggc agcgatagca gcctgcagaa
     2701 cgatatcagc cgctttgttg gttgcatctt ttgcacgcgg gtgcggggag aagataatgg
     2761 cgttacgggt cttcaggctg atcagcgatt tgaagatagc agttgaagtc gggttagtgg
     2821 tcggaacgat accgcaaata ataccgattg gttcagcgat agtgatggta ccaaaagtgt
     2881 cgtcttcaga cagaacacca caggtttttt catctttata ggcgttgtag atatattcag
     2941 aagcaaagtg gtttttgatc actttatctt cgacgatacc catgccggat tcggcaacgg
     3001 ccattttcgc gagtgggatt cgagcatctg cagcagccag agcggcggcg cggaagattt
     3061 tgtctacttg ctcttgagtg aaactggcat attcacgctg ggcttttttt acacgctcta
     3121 cgagtgcgtt aagttcagcg acattagtaa cagccataat gctctcctga taatgttaaa
     3181 cttttttagt aaatcatctg ctcgaatacg agagtatagt cagtgcggtg atgatttgct
     3241 taaccgatgg aaatcaaaag cttactcgcg ctcacactca ctgtgattta ctaaaagagt
     3301 ttaaacatta gagttattat ctctaatgcg tcacttccag gtggcgtaag caagattact
     3361 cacttctggg tactgattac gtgatccaaa tcaaaatttt gcaaagctga cacctttcag
     3421 catggctttt cgccattata gctaacagtt aataaattgt agtatgattt gttggctaca
     3481 ttagcatgtt ttgcacaact agataacaat aacgaatgat agcaatttta agtagttagg
     3541 aggtgaaaaa tgctgtcaaa aggcgtattg tcagcgcgtc ttttcaacct tatttatggc
     3601 taacattatc cggcttttgc ttcggagcta accgtgattc agaccctttt tgattttccc
     3661 gtttacttca aatttttcat cgggttattt gcgctggtca acccggtagg gattattccc
     3721 gtctttatca gcatgaccag ttatcagaca gcggcagcgc gaaacaaaac taaccttaca
     3781 gccaacctgt ctgtggccat tatcttgtgg atctcgcttt ttctcggcga cacgattcta
     3841 caactttttg gtatatcaat tgattcattc cgtatcgccg gtggcatcct ggtggtgaca
     3901 atagcgatgt cgatgatcag cggcaagctt ggtgaggata aacagaacaa gcaagaaaaa
     3961 tcagaaaccg cggtacgtga aagcattggt gtggtgccat tggcgttgcc gttgatggcg
     4021 gggccagggg cgatcagttc taccatcgtc tggggtacgc gttatcacag cattagctat
     4081 ctgtttggtt tctttgtggc tattgcattg ttcgctttat gttgctgggg attgtttcgt
     4141 atggcaccgt ggctggtacg agttttacgc cagaccggca tcaacgtgat tacgcgtatt
     4201 atggggctat tgctgatggc gttggggatt gaatttatcg ttactggtat taaggggatt
     4261 ttccccggcc tgcttaatta attcctttca aatgaaacgg agctgccatg ctccgtttac
     4321 ttcgtcatta tttttacttt gttcctgcac agttatcaaa agcaaaagga ataggtaaaa
     4381 atattcttct caaattacag ttagttataa ggatttcctt aactgcttct cctcaccatc
     4441 atgttatttt cgccacatca taatcctggg cttgctgaag aataattgaa atgatattag
     4501 taattccact gcctttggta gaggaaagtg ctaaataata atcaattgtt aaattattgt
     4561 gtatttcact gctggaactg taatcagaaa agagagacat gcttaaccaa cctctatttg
     4621 attgaattga aagatgtttg ttaaggcatg gatgcaagct atagattctg atacggtcaa
     4681 taaaagagaa ttgcttaaca attttgcaaa atgtattggc gagtaagaac cgcatttggt
     4741 actttccggg caaccgccag gcgattcttt attggtaatg agaataatta acaattaaag
     4801 atcgtcgcga aagaataatg tgtctcgaca ggggagacac agtacgaatc gacataaggt
     4861 gatcgtctga atcaccagaa taaataaagt cggtgatagt aatacgtaac gataaagtaa
     4921 cctgacagca gaaagtctcc gagcctgtgc agggtcccaa tccgggatta cacatgctgg
     4981 ttaataccag taattataat gagggagtcc aaaaaacaat gaccaacatc accaagagaa
     5041 gtttagtagc agctggcgtt ttggctgcgc ttatggcagg gaatgtcgcg ctggcagctg
     5101 atgtacccgc aggtgtcaca ctggcggaaa aacaaacgct ggtacgtaac aatggttcag
     5161 aagttcagtc attagatccg cacaaaattg aaggtgttcc ggagtctaat atcagccgag
     5221 acctgtttga aggcttactg gtcagcgatc ttgacggtca tccggcacct ggcgtcgctg
     5281 aatcctggga taataaagac gcgaaagtct ggaccttcca tttgcgtaaa gatgcgaaat
     5341 ggtctgatgg cacgccagtt acagcacaag actttgtgta tagctggcaa cgttctgttg
     5401 atccaaacac tgcttctccg tatgccagtt atctgcaata tgggcatatc gccggtattg
     5461 atgaaattct tgaagggaaa aaaccgatta ccgatctcgg cgtgaaagct attgatgatc
     5521 acacattaga agtcacctta agtgaacctg ttccgtactt ctataaatta cttgttcacc
     5581 catcaacttc accggtgcca aaagccgcta tcgagaaatt cggcgaaaaa tggacccagc
     5641 ctggtaatat cgtcaccaac ggtgcctata ccttaaaaga ttgggtcgta aacgaacgaa
     5701 tcgttcttga acgcagcccg acctactgga acaacgcgaa aaccgttatt aaccaggtaa
     5761 cctatttacc tattgcttct gaagttaccg atgtcaaccg ctaccgtagt ggtgaaatcg
     5821 acatgactta taacaacatg ccgatcgaat tgttccagaa gctgaaaaaa gagatcccgg
     5881 acgaagttca cgttgatcca tacctgtgca cttactatta cgaaattaac aaccagaaac
     5941 cgccatttaa tgacgtgcgt gtgcgtaccg cactgaaatt gggtatggac cgcgatatca
     6001 ttgttaataa agtgaaagcg cagggcgaca tgcccgccta tggttacact ccaccatata
     6061 ctgatggcgc aaaattgact cagccggaat ggtttggctg gagccaggaa aaacgtaacg
     6121 aagaagcgaa aaaactgctg gctgaagcgg gttataccgc agacaaaccg ttgaccatca
     6181 acctgttgta taacacctcc gatctgcata aaaagctggc gattgctgcc tcttcattgt
     6241 ggaagaaaaa cattggtgta aacgtcaaac tggttaacca ggagtggaaa acgttcctcg
     6301 acacccgtca ccagggcact tttgatgtgg cccgtgcagg ctggtgtgca gactacaacg
     6361 aaccaacttc cttcctgaac accatgctgt cgaatagctc aatgaatacc gcgcattata
     6421 agagcccggc ctttgacagc attatggcgg aaacgctgaa agtgactgac gaggcgcagc
     6481 gtacagctct gtacactaaa gcagaacaac agctggataa ggattcggcc attgttcctg
     6541 tttattacta cgtgaatgcg cgtttggtga aaccgtgggt tggtggctat accggcaaag
     6601 atccgctgga taatacctac acccggaata tgtacattgt gaagcactaa tggcaatacg
     6661 tggggcagga gtgtcctgct ccacggtgtc tgatttttat cgcattacag aaggcacagg
     6721 ccagaaggta gggcaatgtt aaaatttatt ctacgtcgct gtctggaagc gattccgacg
     6781 ctatttattc ttattactat ttcgttcttt atgatgcgcc tcgctccggg cagtcctttt
     6841 accggcgaac gtactttacc accggaagtg atggccaata tcgaagcgaa atatcatctt
     6901 aatgatccga tcatgacaca gtatttcagc tacctgaaac aactggcgca tggcgatttc
     6961 ggtccatcgt ttaaatataa agattattcg gtcaatgacc tggttgcatc cagttttccc
     7021 gtttctgcca aactgggagc cgcagcattt tttcttgcgg taatactggg tgttagtgct
     7081 ggcgttattg ccgcattaaa gcaaaacacc aaatgggact ataccgtgat ggggctggca
     7141 atgaccgggg ttgttatccc cagttttgtg gttgcgccat tattagtcat gatatttgcg
     7201 atcattttgc attggctgcc gggcggtggc tggaatggtg gagcgcttaa attcatgata
     7261 ttgccaatgg tggcgttatc actcgcctat atcgccagta ttgcgcgtat tacccgtggt
     7321 tctatgattg aggttttaca ctccaacttt attcgtactg cccgggcgaa agggttacct
     7381 atgcggcgga tcattttacg ccacgcatta aaacctgctc tgttacccgt gctctcctat
     7441 atggggcccg catttgtcgg cattattacc ggttcgatgg tcatcgaaac catttatggt
     7501 ttaccgggga ttgggcaatt gttcgttaat ggcgcattga accgtgacta ttccttagtg
     7561 ttaagtctga ccattctggt tggcgcgtta accattttgt ttaatgccat tgtcgatgtg
     7621 ctatatgcgg ttatcgaccc gaaaatccgt tactgatact ggagctcgcg atgatgttaa
     7681 gtaagaaaaa cagcgagacg ctggaaaatt tcagtgaaaa gctggaggtc gaagggcgca
     7741 gcttgtggca ggacgcacgt cgacgtttta tgcataaccg tgcggcggtt gccagtctga
     7801 tagtgctggt gctgatcgcg ttatttgtaa tcctggcacc gatgctttcg cagtttgcct
     7861 atgacgatac tgactgggcg atgatgtcca gcgccccgga tatggagtcc ggtcactact
     7921 ttggtactga ctcatccggt cgcgacctgc ttgtgcgcgt tgcgattggc gggcgtatct
     7981 cactcatggt cggtgttgct gcggcactgg tggcagtggt cgtggggaca ctttatggtt
     8041 cgctttccgg ttatctgggc ggtaaagtgg attcggtaat gatgcgtctg ctggaaatcc
     8101 tcaactcctt cccattcatg ttcttcgtca ttttgctggt gacctttttc ggtcaaaaca
     8161 tcctgctgat tttcgtggcg attggcatgg tttcctggct ggatatggct cgtattgtgc
     8221 gtgggcaaac cctgagtctg aagcgcaaag agtttattga ggcggcacaa gttggcggtg
     8281 tatcgacgcc gggcattgtt attcgccaca ttgtgccgaa cgtactcggt gtggtggtgg
     8341 tctatgcgtc gcttctggtg cccagcatga tcctctttga atctttcctt agcttcctgg
     8401 gattaggtac gcaagagccg ttaagcagct ggggtgcatt gctgagtgat ggcgcgaact
     8461 cgatggaagt ctctccatgg ttactgttgt tcccagcggg attcctcgtg gtaacgctgt
     8521 tttgtttcaa ctttatcggc gatggcttgc gtgatgccct cgacccgaaa gatcgttaag
     8581 gagtgcagcc atgagcgtaa ttgaaactgc aactgtgccg ctcgcacaac aacaggctga
     8641 cgcactgctg aacgtgaaag atttgcgtgt cacctttagt accccggatg gcgacgtcac
     8701 ggcggtcaat gatttgaatt tttcgctacg tgccggagaa acgctgggca tcgtaggtga
     8761 gtctggttcg ggcaaatcgc aaaccgcatt tgcgttgatg ggcctgttgg ctgccaacgg
     8821 acgtattggc ggatcggcaa ccttcaatgg gcgtgaaatc ctcaatttgc cagagcgtga
     8881 actcaataaa ctgcgagctg aacaaatctc aatgattttt caggacccaa tgacttcgtt
     8941 gaatccctat atgcgcgtcg gtaagcagtt gatggaagtg ctgatgctgc ataagaacat
     9001 gagcaaagct gaggcgtttg aagaatcggt gcggatgctc gatgcggtaa aaatgccgga
     9061 agcgcgtaaa cgcatgaaaa tgtacccgca cgaattttct ggcggcatgc gtcagcgagt
     9121 catgattgcg atggcattgc tatgtcgacc taagctgctg attgcggatg aacccactac
     9181 tgcgctggac gtcaccgtac aggcgcagat catgacgcta ttgaatgaac tgaagcggga
     9241 atttaatacc gccatcatta tgattaccca cgatcttggc gtggtggcgg ggatctgcga
     9301 caaagtgctg gtaatgtacg ccgggcgcac gatggaatat ggcaacgcgc gcgatgtctt
     9361 ttatcaaccc gtacatcctt attctatcgg tttgctcaac gcggtgccgc gtctcgatgc
     9421 ggaaggtgaa acaatgttga ctattccggg taatccgcca aacctgctgc gattaccgaa
     9481 aggttgcccg ttccagccac gttgtccgca tgcgatggac atttgtagta gcgcaccgcc
     9541 gctggaagag tttacgcctg gccgtctgcg tgcttgcttt aaaccggtgg aggaactgtt
     9601 atgaatgctg aaaccgaagg aagaaaagtc ctcctcgaaa tcgccgatct gaaagtgcac
     9661 tttgaaatca aagatggcaa acagtggttc tggcaaccgc cgaaaacgct caaagccgtc
     9721 gatggtgtaa ctcttcgcct gtatgaaggg gaaacattag gtgtggtagg ggaatcggga
     9781 tgcggtaagt ccacctttgc tcgcgccatc attggcctgg ttaaagcgac cgacggtcat
     9841 gttgcctggt taggtaaaga gttgctgggc atgaagcccg atgaatggcg tgccgtacgc
     9901 agtgatattc agatgatttt ccaggatccg ttggcatcgc tgaacccgcg tatgaccatc
     9961 ggcgagatca tcgctgaacc actgcgtact tatcatccga aaatgtcacg ccaggaagtt
    10021 cgcgagcgcg tgaaggcgat gatgctgaaa gtcggtttgt tgcctaatct gattaaccgc
    10081 tatccgcatg agttctccgg tgggcagtgc cagcgtatcg ggattgctcg tgctcttatt
    10141 cttgaaccga agctgattat ctgtgatgag ccggtgtcgg cgctggacgt gtcaattcag
    10201 gcgcaggtgg tcaacctgct ccagcagttg caacgtgaga tgggattgtc attaattttt
    10261 atcgctcacg acctggccgt ggtaaaacac atttccgatc gtgtgctggt gatgtatctc
    10321 ggccatgcgg tagaactggg gacctatgat gaggtctacc acaatccgct acatccttac
    10381 accagggcat tgatgtcggc agtccccata cctgatccgg atctggagaa gaacaaaacc
    10441 atccagttac tggaagggga attaccgtcg ccgatcaacc cgccttccgg ttgtgttttc
    10501 cgtacccgtt gtccgattgc cggtccggag tgcgccaaaa cacgtcctgt gctggaaggg
    10561 agtttcagac acgccgtttc ttgcctgaaa gtcgatccgc tttaaaagca ataagggctg
    10621 acagttgtca gcccttattc acgccaaaag cgattattta ttcccgccag atgatatggc
    10681 aaagtttgtg atctttttcg cggcataaca gaatgcgcgc gaaaacatcg ttgatttcac
    10741 catcttcact gtccgccaga ccaatcacca cttcggcaaa aaagtccggg ttcaaatcga
    10801 aatcaacatg ttcctgccag tcttccgccg gatcgaataa ctccgcgccg ccgcgctctt
    10861 caaactgaag attgaacagc agaacatcag ctggatcgag attgtcggca gccagttcga
    10921 gaaaaatatc gtaggcctgc tcaagcgttt catcttcagt caggcgattg tttagatcca
    10981 tatccatagt cactacctgt ttaacctctg ttggcgacgt tttacagcaa cgggctgaag
    11041 aagtaaaaca gtcgctcggc gacacgttgc cataatggac gttttagcca taaacgggca
    11101 tcgagcagac gagaacgcga aatataatcg tcctgaacgg cggcgaggtc tgcaccaaaa
    11161 cctttatcgt cgattgccag ggtaatctcg aaatttagcc acagactccg catatcaagg
    11221 ttaacggtgc caaccagact tagttctcca tcgaccagca cgctcttggt atgcagtaac
    11281 ccgccttcaa actgataaat tttaacccca gcagccagca gttctgtaaa gaatgcgcga
    11341 ctggcccagc cgaccagcat cgagtcattt tttcgcggaa ggataatact gacatccact
    11401 ccgcgctgcg ccgccgtgca aatcgcatga agtaaatcat cgcttggcac aaaatagggc
    11461 gttgtcatga tcaaatattc acgagccgaa taagcagcag tcaataatgc ctggtgaatg
    11521 agatcttccg gaaagccggg gccagaagca attgtgtgaa tggtgtgacc gctggcctgc
    11581 tcaaacggca taatattgac atctggtggc ggcggcagaa tacgtttccc ggtttcaatc
    11641 tcccagtcgc aggaataaat aatccccatc gcggtggcga tggggccttc catacgcgcc
    11701 atcagatcaa tccattgccc tacgcccgca tcttgtttga agtagcgagg gtcgaccata
    11761 ttcatgctgc cggtgtacgc gatgtaatta tcgatcatga tcatcttgcg atgttggcgc
    11821 aggtccatac ggcgtaaaaa cacacgcatc agattgacct ttaaggcttc gacaacttca
    11881 ataccggcat tacgcattag ctcgggccat gggctgcgga aaaaagccac actcccggcg
    11941 gagtcgagca tcaatcggca atgaatacca cgccgtgcag cggccattaa tgattcagcc
    12001 acctggtccg ccatgccgcc gggctgccag atataaaaca ccatctcaat attatggcgc
    12061 gcgagctgga tgtcgcggat taacgcctgc atcacatcat ctgactcggt catcagttgt
    12121 agctgattcc ctttgacccc agcgatcccc tgacgacgct cgcaaagctt gaataatggc
    12181 gcagcgacac tgctattttc ttcggcgaag atatgcttac aggctttaag gtcgttaagc
    12241 cattttgcgg tggaaggcca catcgctctg gcgcgctcag cgcggcgttt gcctaaatgg
    12301 agctcgccaa cggcaagata ggcaataatt ccgactaacg gcagaatgta aataatcaac
    12361 agccaggcca tcgcggaggg aactgcgcgt cgtttcatta gaatgcgtaa agttacgcct
    12421 gcaatgagca accagtatcc cagaatggcc aaccaactca ccaacgtata aacggttgtc
    12481 atagattaga aatccttttg aaagcgcatt gttatgagtt tacgcatatg gattcatctg
    12541 gcaaataaaa acgcgggaaa agcactggtc agcgtcaggg gcgggggtat aatgaccatt
    12601 ctgttgttgc atagagtagt taacatgaag cggagtagaa cggaagtggg gcgctggcgc
    12661 atgcagcgtc aggctagccg acgtaaatcg cgttggcttg aggggcagtc gcgccgaaat
    12721 atgcgtatcc acagcatcag gaagtgcatt ctaaacaaac agcgtaactc gttattgttt
    12781 gcgatctaca atatctaaat gtactgggca ccattgcggt gcccaataag tttactcttc
    12841 cgtctttaaa ctctattttt gcgccgattc tttactgtca gctttcgttt cttgttcatc
    12901 gttatcgctg aaaatgccat gaccggagtt caacaacatt gaaacgagca tatcattatt
    12961 aatctcttca ccatttaaaa ctcgcgccag aatttcgctg ccaaacagtt gtggtgagag
    13021 gatgatatcc ggatgtacca tcttaatctt attcaggttt ttgctatcac tgacggcgag
    13081 aactgttttg acatcactgc tcatatcttt tgccgagagt acaacaaacg cgttatctgc
    13141 atcgttatca ctcagcgcca gaatggcccg gcaacgatcg attcccgctt tctttaacac
    13201 tgaactgtca ttactgtcac cggggataac atcggcgtta tcgcctaagc gttgctcaag
    13261 ttgcttgata tcatcttcag gcaagttgct gataaccgtt acgttttgtc cgcgttgatt
    13321 cagttgcaga atcgtattga tggcgagaat cgaatgtccg caaacaataa aatgatcttt
    13381 acgatgcatt gtatgattgt ttccttttac aagtttgttg aatcccccgc ggataagcgg
    13441 accaaaaatt gaggtcatag atgtggcaaa aacggtaatg ccggaaataa tgaccgaaat
    13501 agtgaacaat cgtgctgatt cagaaacagg gacaatatcg ccatagccga cggttgacat
    13561 ggtttctatc gaaaaataga acgcggtcat caaactttct attcgcggat taaaaccttc
    13621 gcttaaataa agcgcaccgt aagtggagta aaacagtaac gtcgtgaaac taataaatgc
    13681 gaaaattgtc ccggctgctg cgctactgtg ggagaagtct ttgcgcagta tcagcaaaaa
    13741 caccagcgta aaaatgcaaa atccaatact aaatttcagc cagggataaa aatgcagagt
    13801 gtaaattagc gctatcaaca acaaaatgat actgattgcc catgcgagct ttgcccggaa
    13861 cagtaagcca atggagttca ggacaagaaa tacgccgagc ataaatagtg gggcgtttgc
    13921 cagcaaggag agatccagct ctgaaaatgc tttgatatgg aaaatatcaa gcaaattgac
    13981 cgacatacca tagattgttt taaaaataag caatccattt aaaaagacgg ccagcgcgag
    14041 aatgtcgtgc cgtagggtca cccataactt tgttgctgtt tgtttgaatg tagtccagtg
    14101 actcactgat acctctgtaa ccacaatttt gtttaattat ctcaacgtgc acattttctg
    14161 aacatacatg cagcgcgtga atgtgttaaa caatagtaga tgactttatc cgcagtaatc
    14221 aaaggttttg agtgagcaac tctgcgtcag gaaagggcat tttcctgaat atgttcacat
    14281 catataaaga aatacagata aagttattat ctgcttgtgg tggtgaatgc actgaccgac
    14341 ctataaggaa aggccaaaca agaacacggt tgcaaaaacc gtgcccttaa atattgaatt
    14401 tctattcaga acactttctt aaatggtttc actgaaacgt gttcatagac tcctgccgct
    14461 acgtacgggt cagcatcggc ccaggcctga gctgcttcca gcgactcaaa ttcagcaata
    14521 acggttgagc cagtaaatcc cgcagcccct ggatcgttac tgtctaccgc tggcattgga
    14581 ccggcggtca acaaacgacc ttcatcatgc agtaactgta aacgtgctaa atgtgccgga
    14641 cgaacggaaa ggcgtttttc gaggctatca gctttgtctt gagcgtaaat aacatacaac
    14701 acaggcacaa ctccttgttc gggaaagttg taaagtacgt tatttgaaag ggcgaagatc
    14761 tgcaacggaa agatgatgtc tttgttaaga ccatgcataa agtaaggtca attacgccaa
    14821 aaatgacatt ttcactgatc ctgatcgtct tgccttattg aatatgattg ctatttgcat
    14881 ttaaaatcgg ggcctggttt ttctactgaa atgattatga cttcaatgac ccttgattta
    14941 cctcgccgct tcccctggcc gacgttactt tcggtctgca ttcatggtgc tgttgtggcg
    15001 ggtttgctct atacctcggt acatcaggtt attgaactac ctgcgcccgc gcagccgatt
    15061 tctgtcacga tggttgcgcc agcggatctc gaaccgccac aagccgttca accaccaccg
    15121 gagccggtgg tagagccaga accggaacct gagccgatcc ctgaaccgcc aaaagaagct
    15181 ccggtggtca ttgaaaagcc gaagccgaaa cctaagccaa aaccgaagcc ggtgaaaaag
    15241 gtggagcagc caaaacgcga tgtcaaaccc gtagagtcgc gtccggcatc accgtttgaa
    15301 aatacggcac ccgcgcgccc gacatcgagt acggcaacgg ctgcaaccag caagccggtt
    15361 accagtgtgg cttcaggacc acgcgcatta agccgtaatc agccgcagta tccggcacga
    15421 gcacaggcat tgcgcattga agggcaggtt aaagttaaat ttgatgtcac gccagatggt
    15481 cgcgtggata acgtacaaat cctctcagcc aagcctgcaa acatgtttga gcgtgaggtg
    15541 aaaaatgcga tgcgcagatg gcgttatgag ccgggtaagc caggcagtgg gattgtggtg
    15601 aatatcctgt ttaaaattaa cggcactacc gaaattcagt aagcataaag tcaaaagcct
    15661 ccgaccggag gcttttgatt attactcaat gggtaaggcg cgaggttttc cttcgggatc
    15721 aaccgcgaca tacttaaata atgcttctgt tgctttatag cgttgcccga ttggttcaga
    15781 cgccactttt ttcacccaca cttcaatatt aatgctgact gatgtcgtcc ctttctggat
    15841 acagcgtgca tagcagcaca ccacatcgcc gaccgcaacc ggccgtaaga aagtcattcc
    15901 ttcaacccgc acggtcacta cacgaccatg ggcaatttct ttcgccagaa tagcgccgcc
    15961 aatatccatt tgtgacatta accaaccacc aaagatgtca ccattggcat tggtatcggc
    16021 gggcatggct aaagtacgta aaacaagatc gccctgaggg acgttatgtg ttgtagacat
    16081 ggtaaaaccg acttaaaagg aaaatcacag gcgcgatgct actatgattt tcctgaggag
    16141 aacagaggag atacagcgcc agcccctgaa ggactggcag tctggttagg atttatcttc
    16201 ctgcggcatg tggcggtaga tatagatacc gcttaacaat gtgaagatta aggtaagggc
    16261 ggtcaggcca aagactttaa agttgaccca aatattttgc ggcagccaga atgcgatgta
    16321 gatgtttgcc agaccgcaaa ggataaagaa aacagcccag gccagattca gcttcgacca
    16381 taccgattgc ggcagggtga gttctttacc cagcatccgc tgaattagcg gctttttcat
    16441 cacccattgg ctgactaaca gggcacccgc aaacagggca taaatgacag taaccttcca
    16501 tttaataaac tcatcattgt ggaagaacag cgtcaagcca ccgaagacga ccaccagaac
    16561 aaaagtgatc agggccatct tctcaacctt acgaaagcga acccagctat atataagcac
    16621 aatcgccgtg gcgacgatga gcgccgcagt agccgcatag atgtcataaa tcttgtaaaa
    16681 cgcgaaaaag acaaccagcg gtaaaaaatc aagaaactgc ttcattttac gattccgtaa
    16741 tcaaatgctt aaggttattg gcgaatcaac atatacaggc ggaacagata gatgagcaat
    16801 acggctgaaa tcaggttgct caaggtgttc gccagtacgg caccaatttc cggggttaat
    16861 gcggcaaaag aagaggcaaa aagcagcaac agtgtttttg ccagcaacca gctcagtact
    16921 gcgggtgcca ccagacgcat attcgcccat gtcagccgca tactgctacg catcgaggca
    16981 aaaacgccca ttttgtcctg taccagcatc accggtgcca gagccagtaa aatggccatg
    17041 ataattcccg gaacgaccac cagcatgatg ccaatctgta ctaaaagggt agtcagaaaa
    17101 atcagaataa ataactttgg caatatcggc gcactggcac caatagctcg cagcgcactg
    17161 actctctgac ccgcagacac cagctggata attaatatta cgcccccggc gagaatggcg
    17221 ttaccgatta atcctgaaaa agtggacgcc gctgaagcct gcagcaaaat ttgttgctgt
    17281 tccggtgaca tattctgaac caggtcgaac aacccactac tgccgctaac gggtacgccg
    17341 tcattgagct gcgcaagctg tgcatcactg ggtgagaaaa catgccctaa caccactgtg
    17401 ataaacgcac atagcaacga taccaacaga atggtcataa attgattacg gaaaaaattt
    17461 ccggtgtcac ggtatacgga ctgcgccgtg atagacatgc actctccttg agttttatgc
    17521 aggtgttaat tagcgggcaa ttgtaccctg gttaagcccc gggtggcagc atcaggtctt
    17581 gtatggaaaa gcatatcttt gtaaagcgga ggtaattgct ggccttcagg cgtgggcaaa
    17641 ccgctgcaag tggcgagcga gttaatattt gcgtagcgaa atatataaaa attgatttaa
    17701 atcacattaa ccaggatttt caatgcaact tctaaattaa tccagatcaa taaagggtga
    17761 attaccatat gtaatgtgat ctgtgtagga tcatttgtta ccccaatgtg ggtatattcg
    17821 tcacgttttt ataaccataa cgacggagcg gatatgaaaa agttaacagt ggcggctttg
    17881 gcagtagcaa ctcttctctc tggcagtgcc tttgcgcatg aagccggcga attttttatg
    17941 cgtgcaggtt ctgcaaccgt acgtccaaca gaaggtgctg gtggtacatt aggaagtctg
    18001 ggtggattca gcgtgaccaa taacactcaa ctgggcctga cgtttactta tatggcgacc
    18061 gacaacattg gtgtggaatt actggcggca tcgccgtttc gtcataaagt tggcacagcc
    18121 actaccgggg atattgcaac tgtccaccat ttaccgccaa cactgatggc acaatggtat
    18181 tttggcgatt caggcagtaa attccgtcct tacattggtg cgggtattaa ctacaccacc
    18241 ttttttaata atgacttcaa tgacaccggt gaaggtcttg gtttatctga ccttagtctg
    18301 aaggattcat ggggcgtggc ggggcaagta ggtctggatt atctcatcaa ccgtgactgg
    18361 ttgctgaata tgtcggtctg gtacatggat atcgataccg aagtgaaata taaatccaac
    18421 ggcgcagtgc tgcctgcagg gcgttattct gacaacgtac gccttgaccc gtgggtattt
    18481 atgttctctg cgggttaccg cttctaattt tttaccatca ataaaccgcc agatgttttc
    18541 tggcggtttg tctcaaatta actgcgcgtc gccgctttca tcggttgtac aaaagctttc
    18601 agtgccgcca gcattttctc tggctcatta atatgttgct cgatgatttt aacgatggcc
    18661 gaaccagaaa tcgcgcccgc agctcctgca tcaatcgcgg cttttacctg atccggggcg
    18721 gaaataccaa atccctgcaa tggaggcgca gcgttgtact ctttcagctt cgcaaccaga
    18781 tgattgaggg gtaacgcggc gcggttttct gcgccggtca cgcccgctcg cgacagcaaa
    18841 taggtgtaac cacgaccgta agaggctatc tggcgcagca aatcatcgtc ggcattcggc
    18901 gggcaaataa agataggtgc gacattatga cgcaacgcgg cctggcggaa gggcgcggac
    18961 tcttccacgg gcacatcggc aaccagcacc gaatcgacgc cgactttctc gcactcggca
    19021 taaaactcat caatgccttt gttaaacacc aggttggcat acatcaaaag gccgatggga
    19081 atggtcgggt gcttctggcg aatgagtgcc agcacctcaa agcactgcgc cggggttact
    19141 cccgccgcaa aagcacgcag tgtggcgttt tgaatcgtcg ggccatccgc cagtgggtcg
    19201 gagaagggga tgcctaactc cagcgcgtca gcaccggctt caattagcgt atcgataatt
    19261 ttcaacgact gctcaatgcc cggatcaccg agggtgacga aaggaacgaa tgcgccttct
    19321 ttgcgctcct tcaactgggc aaacagagat tcgtagcgtt ccatcagatt tcccctcgtg
    19381 ctttcaaaat atcgtgaacg gtgaagatgt ctttatcgcc gcgaccggaa aggttaacca
    19441 ccagtagctg ctctttttcc ggattttcgc gcatcatttt cagcgcatgg gccagggcgt
    19501 gggaggattc cagcgccggg atgatccctt catgcaggca aagcgtttta aaggcttcca
    19561 gggcttcatc gtcggtaata gacacgtaat cagcgcgtcc agtgctgttg agatacgcat
    19621 gttgcgggcc gacggacggg aaatccagcc cggcagaaat ggagtaagac tcttcaattt
    19681 gcccgtcttc ggtttgcatc atcggcgctt tcataccgaa atagatgccc acgcgaccat
    19741 gttttaacgg tgcgccgtgc tcgccagttt cgataccgtg gccgccaggc tccacaccaa
    19801 tcaggccgac gtcggtttcg ttgatgaaat ctgcaaacat accgatggca ttcgaaccac
    19861 cgccaacaca ggcgataacg gcatccggca ggcgaccttc tctttccaga atctgcgctt
    19921 tcgtttcttc gccaatcatc cgctgaaact cacgcacaat ggtcgggtaa ggatgcgggc
    19981 cagctgcggt acccagcata tagtgcgcgg tttcataact gccggaccag tcgcgtagcg
    20041 cctcattaca ggcatctttc agggtcgcgg aaccgctatg taccgggatc acttccgcac
    20101 ccattaagcg catccggaaa acgttgggcg actggcgttc aacgtctttg gcacccatat
    20161 aaatacggca tttcaggccg agcagggcgc tggcaagggc cgacgccacg ccatgctgac
    20221 cggcaccggt ttcggcaata atttcagttt tacccatccg cttcgccagt aaagcctgac
    20281 cgagcacctg gttagtttta tgcgcgccgc cgtgcagcaa atcttcgcgc ttcagataca
    20341 gcgtggtgtt cgtcccggct gtaatgttct ggcatttggt cagcgcggtt ggacgcccgg
    20401 catagttttt cagcaggtcg ttgaactgag cctgaaattc aggatctttt tgcgcgctga
    20461 caaaagcttc ttccagctgg cgcagagcag gcatcaggat ttgtggcacg tacatgccgc
    20521 caaactcacc aaaatagggg ttaagtaatg ttgtcattgt tcctttcctt aatatgcgcg
    20581 cagcgtctgg aaaaccgagg ccaaaagacg tgcgtctttg atacccggtt gcgactctac
    20641 agcagaatta aaatcaagcc cggcgcagcc ggtttgtgcc gcttccacgc agttatctgc
    20701 gcctaagccc cccgccagca gaacgttgcc aagcgattga ccatttaata gtgaccagtc
    20761 gaaacgttgt ccgctcccgc cctgaccgtt gtcgaataca tatttatcga tgtgctgaaa
    20821 atcgcgcgcg ggaagagttt caccgacact taaagccttc cagatggcga cgtgtgctgg
    20881 cagagcctca cgcagagtgt cgatatacag ctgatcttca ttaccatgca gttgcactgc
    20941 cgccagcgat aacaccttag ctttgtccac cacatcggca atatcgtgat tgcggaacac
    21001 gccaacatac tgcaacggtg ccgcagccat cacttcctgc gcctgttcaa cgttgacgca
    21061 acgcggtgat gtcgcaacaa aaatcaaccc accgtaaatc gcgcccgcgt cataagcagc
    21121 tttagcatct tgcccacgtg tcaggccaca tactttattc tcacccagca acacccgacg
    21181 cacggtggcg ttcaaatcgt catgggccat caacgccgaa ccaatcagaa agccgttagc
    21241 gaagtggctt aactcgcgca cctgagcgta agtattgatg ccggattcgc tgattaccgt
    21301 cacgttgtgc cccagtttcg gtgcaagctc gcgggtacgg ttgagatcaa tcgacaaatc
    21361 gcgcagatcg cggttgttga tgccaacgac ctttgccccc aatgcaatgg cgcgctccag
    21421 ttcctcttca ttactgactt cggtcagcac acccatctcc agactgtggg cgacggctgc
    21481 aagctggcga tattgttcgt cattcagtac tgaaagcatt aataagcagg catcggcctg
    21541 gtaatagcgc gccagataga tctggtaagg atcgataatg aagtctttac ataaaatcgg
    21601 ctgcggggcg atttggctga cgatggggag gaaatcaaag ctcccctgaa aatatttctc
    21661 atcagtcagc actgaaattg ccgaagcgta atgtttataa atggcagcaa tgcgtgccgg
    21721 atcgaaatca tcacggatca cgccttttga cggcgacgct tttttgcact ccagaataaa
    21781 cgccgtgcgt gcaccctgta gcgcatcata aaaatgtcgc gtgctcggct gaatctcatt
    21841 ctgaaaactg gccagcggtt gctgctgttt gcgggcttct acccaaatcg ccttgtctgc
    21901 gacgattttc gctaaaacgg tttgcattat ttaccctcgt gccgccagtg cggtgactct
    21961 gtcgtaagcg gaaccactgc gcagtacctc aagaacggtt tgcgcattgg cttgcagatc
    22021 ttcatggcca tgcaggcgca ttaacatggc gacattcgcc gcgacggctg cttcgtgagc
    22081 ggcgtcgcct ttaccttgta acaagcgtgt taaaatgtca cggttttctt ccggtgttcc
    22141 gcctgccagt tgctcctggt ggtagggtgt caggccaaaa tcttcagcgg tcaattgata
    22201 gctcttaatt tcgccgtcat gcagttcggc aacgattgtc ggcgcgtgta atgaaacttc
    22261 atccatcccg ccgctgtgca ccaccgccgc gcgttgatac cccagcacgc gcaaggtttc
    22321 ggcaatcggc agcaccagtt ccggactata aacaccaatt agcgccagcg gaggatgcgc
    22381 cgggttaatc aatggcccca gcacattgaa cagagtgcgg gttttcagtt gctggcgaac
    22441 cggcatcgca tggcgaaatc cggtgtgata tttcggcgca aagaggaaac atacgcctaa
    22501 ctcatccagc gcctggcgcg atttatcggc gttcatatca agattaatac cgaacgccgc
    22561 cagcagatcc gacgagccgg atttactgga gacgctacgg ttgccgtgtt tcgccacttt
    22621 cagcccgcag gccgcggcga caaacgcact ggcggtagaa atattgatgc tgttactgcc
    22681 gtcaccgccg gtgccgacga tatcggcaaa cagataatcc gggcgcggga acggcgcggc
    22741 gttttccagt agcgcggttg ctgctccggc gatttcgttc gggtgctcgc cgcgaatttt
    22801 catgctcacc agcgccgccg ccagttgttc cggtttcagt tcgccacgta ccaccgctga
    22861 aaacagctgg tggctttctt gttggctaag ggtctgcgcc tgatacagtt tttccagaat
    22921 cggttgcagc gtgttggttg gctcaagttt ctgctgcgcc caggccagcg tttgttccag
    22981 caggcgagcg ccctgggtag taagaatgga ttccggatgg aactggaatc cacaaacgcg
    23041 atccgcatca tgacgcaccg ccatcaccat gccattaaaa tgggcgttga tggttaaacc
    23101 ggccggaata ttactgccaa ccagcgagtg ataacgcgcc actggcagcg ggtttgttaa
    23161 tccggcaaac atcgcctgac cgtcatgttc aatgctcgac gctttaccgt gaagaatttc
    23221 gcccgcctga ccgacatagc ccccgtaagc ttcgacaatc gcctgatgtc cgaggcaaat
    23281 gccaataatt ggcagcttgc cacgcaagcg ggtgaggagt tccggcatac aaccggcttc
    23341 gctcggcaca ccggggccag gagaaagcat cagcaccgga ttgctcatcg tcgccaggcg
    23401 ttcaattaag gtctgcgccg gaatatggtt gcggtaaatc accacgttat ggccattgct
    23461 gcgcaactga tctgccaggt tgtacgtaaa agagtcgata ttatcgagca gcagaatgtc
    23521 agccatcaga aggtctcctg tgcatgatgc gcggtggcaa tagcgcgcag tacagcgcgg
    23581 gctttattac gagtttcgtc ggcttccgac tgcggaacag aatcaaggac tacgccagca
    23641 ccggcttgca cggtggcgat accgttttcc accagcgccg agcggatcac aatgcaggtg
    23701 tcgagatcgc catgcgcggt aaaataacct accgcgccgc cgtagctgcc gcgtcgacga
    23761 ccttctgctt cggcaattaa ctgcatagcg cgtactttcg gtgcaccgct taacgtcccc
    23821 atattcatac aggcgcggta agcgtgcagg gcgtcgagat cgtggcgcag ctcaccaaca
    23881 acgcgggaga ccaggtgcat cacgtaagag taacggtcaa ctttggtgag atcggcgacg
    23941 tagcggctgc cgggtgtgca aatgcgtgcc aggtcattac gggcgagatc caccagcatc
    24001 agatgttcag aaagctcttt atgatcggta cgcatctcca gttcgatgcg gctgtcgagg
    24061 tctctatcca gtgaaccatc ggcacgacga ccgcgtggac gtgttccggc aatcgggtaa
    24121 atctcaatct ggcggctggt ggcgtcatac ttgagcgaac tttccggcga cgcgccaaac
    24181 agggtgaaat cattatcctg cataaaaaac atgtacgggc tgggattact ctttttcagc
    24241 acgtaatagg ccgccagcgg tgacgggcag ggcagagaga aacggcgaga tggcaccacc
    24301 tggaaaattt ctccggcgcg aatcgctttt tgcaacaaac gcactacgcc accgaactct
    24361 tcatcgctct ggttacattc acaacgcata tgcggcacgg aaaccaccgg cagcggcggc
    24421 gcggcttcgg tcagttgctg acgtagttcg ttcaggcgag cagtgagacg ttgtttttct
    24481 tcttcattcg gagcaaacag gctggcctga atacgggtgc tttttttctg atggtcaatc
    24541 accatcagcg tttcagcgag ataaaaacag aaatcagggc agctattttc cgctgacagt
    24601 tgcggtaaat tttcaaatcc cgccacaagg tcataagaga acaggccgcc gaagaacatt
    24661 gcttctcgtt cttccttcgg tacattcaac agattctgta ataagcggaa agcgtcaaaa
    24721 accgaaaggg agcataagcg ggcgtcttca tccagcagtg gactgacagg cgggaagcgc
    24781 agtacgcggc agtttggtga ttgttcattt tccacacccg caggcaaggc gttatccagt
    24841 agtgtcaaca gggcttctcc attgccggaa agcgcctgga ttgtgacagt gtcacttaat
    24901 gctgtaatgc gcagcgcact gtctaccagc agcaggcttt ttaaatcatc tttgctgtcg
    24961 atatctgcgg attccagcag cagcgttgcc gggcgatccc cacacaactg gtgaaaaagc
    25021 gcagtcgggt tgtcgcgata agcgcctttg caggttagca gttcgagagt cggtttttgt
    25081 gtttgcattg ttattctcta attttgttca aaaaaaagcc cgctcattag gcgggctggg
    25141 tatctgattg ctttacgcat ggtgaataca ctgcccgttt caggaagtgc gccaccaacc
    25201 tttcagtacg aaaattgctt tcattgtcga tacccttttt acgtgaactt gcgtactagt
    25261 taactagttc gataattaat tgtcaacagc tcatttcaga atatttgcca gaaccgttat
    25321 gatgtcggcg caaaaaacat tatccagaac gggagtgcgc cttgagcgac acgaattacg
    25381 cagtgattta cgacctgcac agccatacca cagcttccga tggctgcctg acgccagaag
    25441 cattggtgca ccgtgcagtc gagatgcgcg tcggcaccct ggcgatcacc gaccatgaca
    25501 ccacagctgc aattgcacca gcaagagagg aaatttcacg ttcgggactg gcgctgaatc
    25561 ttattcctgg cgtggaaatt tccacggtct gggaaaatca tgaaattcat attgtcgggc
    25621 tgaatattga tattactcat ccgctaatgt gcgagttcct cgcgcagcag acagaacggc
    25681 gcaatcagcg ggcgcagctg attgccgaaa gacttgaaaa agcgcaaata cctggggcac
    25741 tggaaggcgc gcaacgactg gcgcagggcg gtgcggtgac gcgcggtcat tttgcgcgtt
    25801 tccttgtgga gtgcggaaaa gccagttcaa tggcggatgt ctttaaaaag tatctggcgc
    25861 gcgggaaaac cggatacgtt ccgccacagt ggtgtacaat agagcaatct attgatgtca
    25921 ttcatcattc tggcggtaag gcggtgctag ctcatcctgg gcggtacaat cttagtgcta
    25981 aatggctgaa aagattggtt gcgcattttg ccgaacacca cggcgacgcg atggaagtcg
    26041 cgcagtgcca gcaatcgccc aatgaacgca cccagctggc gacacttgcg cgtcagcatc
    26101 atttatgggc atcacaagga tctgattttc atcagccatg cccgtggatc gaactgggtc
    26161 gtaaactctg gctgcccgca ggcgttgaag gcgtctggca gctatgggaa cagccgcaga
    26221 acaccacaga gagggaatta tgagccagtt tttttatatt catcctgata acccgcagca
    26281 acgtctgatc aaccaggcgg tggagatcgt gcgtaaaggc ggggtgattg tttatccaac
    26341 tgattccggc tatgcgctcg gctgtaaaat tgaagataaa aacgcgatgg agcgtatttg
    26401 tcgtattcgc cagctgccgg acggtcacaa ctttaccctg atgtgtcgcg atctttctga
    26461 actctcgacc tattcatttg tcgataacgt tgcgtttcgt ttaatgaaaa acaacacacc
    26521 gggcaactat accttcatcc tgaaggggac gaaggaagtg ccacgccgtc tgttgcagga
    26581 aaaacgcaaa accatcggta tgcgtgtgcc ttctaacccg attgcgcagg cgttgcttga
    26641 agcattgggc gaaccgatgc tttccacttc gctaatgctg ccaggcagcg aatttaccga
    26701 atccgatccg gaagaaatta aagaccgtct ggaaaaacag gtagatttga ttattcacgg
    26761 cggttatctc gggcagaaac caacaacggt tattgatctt accgatgata cgccggtcgt
    26821 ggtgcgtgaa ggcgtaggtg atgtgaagcc tttcttataa caaattgcgt tccatggatg
    26881 ggtgaatatg gcagggatat atcgctgcat tttgctattg atagttgggc tttttttctc
    26941 ttcgttgagt tatgcgaaaa acacggagat cccttcttat gaagaaggga tctcgctctt
    27001 tgatgttgaa gccactctgc aaccggatgg ggtgctcgac atcaaagaga acattcattt
    27061 tcaggcgcga aatcagcaga ttaagcacgg attttatcgt gatttaccac gactatggat
    27121 gcagcctaat ggtgacgctg cactgctgaa ctatcgtatt gttggcgtta cccgtgacgg
    27181 cgttcctgaa ccctggcatc ttgactggca tatcgggtta atgagtattg tcgtgggcga
    27241 taaacaacgt ttcttgcctc aaggcgacta tcattatcaa attcattatc aggtgaaaaa
    27301 tgctttcctg cgcgagggag attctgatct gttaatctgg aacgtgaccg gtaaccactg
    27361 gccgtttgaa atttataaga ccctattttc actcaagttg cctgatattg cgggtaatcc
    27421 atttagcgaa atcgatctct ttactggaga agagggcgac acatatcgaa atggccgcat
    27481 ccttgaggac ggaagaattg aatctcgcga tccgttttat cgtgaagatt tcacggtcct
    27541 ctaccgctgg cctcacgcat tacttggtaa tgccccggct ccacaaacga cgaatatttt
    27601 cagtcatctt cttttaccct ctacgtcatc gttgttaatt tgctttccga gtctgttcct
    27661 ggcgtgtgga tggttatatc tctggaagcg caggccgcaa tttacgccgg tagatgtgat
    27721 tgaaaccgat gtcattccgc cagattacac gcccggcatg ttacgtcttg atgcgaaact
    27781 ggtttacgac gataaaggtt tttgcgccga tatcgtaaat ctaattgtga aaggaaaaat
    27841 tcatctggaa gatcattatg acaagaacca gcaaatcctg attcgtgtta atgaaggcgc
    27901 gaccagaaat aatgcggtaa tactgcccgc agagcagtta ttactggaag cgttatttcg
    27961 taaaggcgat aaggtcgtgc ttacggggag acgcaacaga gtcttacgca aggcattttt
    28021 acggatgcag aaattttatc tgccgcgtaa aaagtcttcg ttttaccgac ctgatgcgtt
    28081 tttgcaatgg ggtggactgg caatattggc ggtcattctc tacggtaacc tgagtcccgt
    28141 aggttgggca ggaatgagtc tggtaggcga tatgtttatt atgatctgct ggattattcc
    28201 ttttttattt tgttcccttg agcttttgtt tgcccgcgat gatgacaagc cttgcgttaa
    28261 tcgtgtaatc atcactttgt ttttaccgct gatttgttca ggcgtggcct tttactctct
    28321 ctatatcaat gtcggagatg tattctttta ctggtatatg ccagcgggtt attttagcgc
    28381 tgttttcctg accggttatc tcactggcat ggggtatatt tttctgccaa agtttaccca
    28441 aactgggcag caacgttatg cccacggtga agctatcgtt aactatcttg cgcgtaaaga
    28501 ggcggcaaca cacagtgggc ggcggcggaa aggggaaaca cggaaactgg attacgcatt
    28561 gttaggttgg gcggtctcgg caaaccttgg cagagaatgg gcagcacgta tcaccccatc
    28621 acttacagcg gctgttcgcg ccccggaaat tgcccgtagt ggcgttttat tctcattaca
    28681 gacgcacctg agttgcgggg cctataccag tttgttgggg cggagctatt ccggtggtgg
    28741 tgctggtggc ggtgcaggtg gcggaggcgg cggtggctgg taatcaagct gatgttaatc
    28801 gaaacaagct ttaaccataa agatagtgta gtatgttgcg cctcaaagcc aggccagtta
    28861 aaacgtcgag tcgtttactt aaggcctgaa gagttcaaac agactttact atcagtgagt
    28921 cagatgtaag ttctgactca ctgttgcaca aaagcatatc agacgcctgg gaaggcgaca
    28981 ccaaaaggaa gctctatgag cgaaaagcta cagaaagtgc tggcgcgtgc cggccacggt
    29041 tctcgccgtg aaatcgaatc tattattgaa gccggtcgtg taagtgttga tggcaaaatt
    29101 gctaaactcg gcgatcgcgt tgaagttacc cctggattga aaatccgtat cgatggtcac
    29161 ctgatttcgg tacgtgagtc cgctgaacaa atttgtcgcg ttctggccta ttacaaaccg
    29221 gaaggtgagt tgtgtacccg taacgatccg gaagggcgtc caacggtgtt tgaccgtctg
    29281 ccaaaactgc gtggcgcacg ctggattgcc gtaggtcgtc tggacgttaa cacctgtggt
    29341 ctgttgctgt tcactaccga cggtgaactg gcaaaccgtt taatgcatcc aagccgtgaa
    29401 gttgaacgtg aatacgccgt gcgcgtattt ggtcaggttg acgacgcgaa actgcgtgat
    29461 ttgagtcgtg gcgtgcagct ggaagatggt ccggcagcct ttaaaaccat caagttcagc
    29521 ggcggcgaag ggatcaacca gtggtacaac gttactctga ccgaaggccg taaccgtgaa
    29581 gttcgtcgtc tgtgggaagc ggttggtgtg caggtaagcc gcctgattcg tgttcgttac
    29641 ggtgatatcc cactgccgaa aggtctgcca cgcggcggct ggacagagct ggatctcgcc
    29701 cagactaact atctgcgcga actggtggag ctaccgccag aaaccagctc taaagtcgct
    29761 gtagaaaaag accgtcgtcg catgaaagca aatcagattc gtcgtgcggt gaaacgtcat
    29821 agccaggtga gcggtggtcg ccgttctggc ggacgtaata ataacggtta atcaaagtat
    29881 gccggacgtc atatccggca tttttacaga ttagtaatca atccctatct gcgcttttac
    29941 accggcatca aacgcatgtt tgacggggcg taattcactt accgtgtccg ccagttcaag
    30001 aatatcccga tgacaaccac gacctgtgat aatcaccgtc tgttgatgtg gacgttcatt
    30061 taacgcctgc accacttctt ccagtggcaa atagtcatac gccaccatat acgtcagttc
    30121 atcgagcaaa accatatcca gtgaggaatc agccagcatc cgctttgcat gttgccagac
    30181 ttcgcggcag gcggcggtat cagactctcg gttttgtgta tcccaggtaa agcccgttgc
    30241 cattacctga aactcaacgc cgtgtggctc cagcagattt cgttcaccat taggccaggt
    30301 gcctttaata aactgcacga cgcccacttt ttttccgtga ccaactgcgc gtgttgccgt
    30361 accaaatgcc gcgctggttt tgccttttcc attgccggta aagacgatga taataccgcg
    30421 ttcatcctgg gcctgggcca cacgagcatc tactttttct ttcactcgct gctgacgctg
    30481 ttggtagcgt tcatcactca ttgggaaatt cctggtttac ggcccggttg ggcgtcaaag
    30541 gtcatgccgg ttttacggcg gctgtcatcg cccatcagcc agaggtagag cggcatgata
    30601 tcagcggggg ttttaagttt ctgtggatct tcggtcggga aggcgctggc ccgcattgcg
    30661 gtgcgcgtac cgcctgggtt aatgcagttg acacgcaggc gctgctgata ttcatcggcc
    30721 agtacctgca tcatcccttc ggtggcaaat ttcgacgctg catatgcgcc ccagtttgca
    30781 cgcccctgac gtccaacgct tgatgaggta aagaccagtg aaccggcatc cgatttgagt
    30841 aataaaggaa gcagtgcctg tgtgagcata aaggtggcat taacgttgac ctgcatgacg
    30901 tcctgccaga cctgcggatc ttgttcgctc attgggcaaa catcgccgag caatccggca
    30961 ttatgcaaaa caccatccag acgcggataa ttaacggcaa tgcgctgtgc cagttgttgg
    31021 caatcttcgg acgtgcaggt tagcaaatcg agaataaacc actgtggctg acgtccaatt
    31081 tcttcgttta tgtggctggc tacctgacgt aatttttctt cattacggcc caaaagaatc
    31141 actgtcgcac catagcgtgc atacgtcatc gctgcttcac gaccaattcc atcgctggct
    31201 cccgtcacca ggataatgcg atcattgagt aaatcttgtt ttggctggta atgcatggct
    31261 actcctcaac gacgttgtct gtcgcgctgc cagtgtactt tatgactttg gggctttatg
    31321 cctgaaacag aagggcattt caatcagcct ggggcgataa caccgcaaaa tggatacttt
    31381 gtcatacttt cgctgcaata acatctctgc gagacggcta aacatgcctg ttgtaaactg
    31441 tgagccaaag cgttgtttaa ccaaggtggg gactcgtgga attgttgtct gaatatggtt
    31501 tgtttttggc gaaaatcgtt accgttgtgc tagcgattgc ggcgattgcc gccattattg
    31561 tcaatgttgc tcaacgtaat aaacgccagc gtggcgagtt acgagtcaac aatctcagcg
    31621 aacagtataa ggagatgaaa gaagaactgg ccgcggcgct gatggacact catcagcaaa
    31681 aacagtggca caaagcgcag aagaaaaagc acaagcaaga agcgaaagcc gcaaaagcga
    31741 aagctaaact gggcgaggtg gcaactgaca gtaaacctcg cgtctgggtg ctggatttta
    31801 aaggcagcat ggacgcccat gaagtgaact cgctacgtga agagataacg gcggtactcg
    31861 cagcattcaa accgcaggat caggttgtgc tccgtctgga aagcccaggt ggcatggtgc
    31921 atggttacgg gttggcggct tcgcagttgc agcgtctgcg tgataaaaac attcctttaa
    31981 ctgttacggt agacaaagtc gctgccagcg gcggttacat gatggcctgt gtggcggaca
    32041 aaattgtttc cgcaccgttt gctattgtgg gttccattgg ggtggtggcg caaatgccca
    32101 actttaaccg cttcctgaaa agcaaagata ttgatatcga actgcacacc gccgggcagt
    32161 ataagcgcac gctgacgttg ctgggtgaaa ataccgaaga agggcgggag aaattccgcg
    32221 aagagttgaa cgaaacgcat cagttattta aagattttgt gaagcgtatg cgtccgtctc
    32281 tggatattga acaggtggca acgggtgaac actggtacgg acaacaggca gtagagaaag
    32341 gcctggttga tgaaatcaac agcagtgatg aagttattct tagcctgatg gaaggccggg
    32401 aagtggtcaa tgtacgctat atgcagcgta aacgactcat tgaccgattc accggcagcg
    32461 cggcagagag cgccgatcga ttgttgttac gctggtggca gcgtgggcaa aagccgttga
    32521 tgtaaaagac aaacgcgagg ataagacctc gcgttttgct ttaatcaacc aggtgatatt
    32581 tttctgaaag cacatgggcc aggtgtttga acatattaaa caccgcggtg cttttggcag
    32641 ttggtaatcc ttgttcatct aaaaagtagt cgccggtaaa taccagtacg ccattacgct
    32701 gcgtgacgcc ggtggcttca atccctgcga gcgtatcctc atgctcacga atgattttgt
    32761 tggcctcttt caacagcgtt tcgcggtcga tgggttgagt ctctttgttc attatttact
    32821 ccttaaacaa ggacattagt ctacgccagg catggcttgc agacaaatat accacgctgg
    32881 tggcaagagc gccttactgg caactttgga ttttgcatgc taataaagtt gcgtatcgga
    32941 ttttatcagg tacagtgtga cgctttcgtc aatctggcaa tagatttgct tgacattcga
    33001 ccaaaattcc gtcgtgctat agcgcctgta ggccaagacc tgttaactca gtcacctgaa
    33061 ttttcgtgag cagagtcacg acaaggggtt gatatccgca gagagcgagt ccatatcggt
    33121 aactcgttgc cagtggaagg tttatcaacg tgcgacgcat tcctggaaga atcaaattag
    33181 gtaaggtgaa tatgggtaaa gctcttgtca tcgttgagtc cccggcaaaa gccaaaacga
    33241 tcaacaagta tctgggtagt gactacgtgg tgaaatccag cgtcggtcac atccgcgatt
    33301 tgccgaccag tggctcaact gccaaaaaga gtgccgactc tacctccacc aagacggcta
    33361 aaaagcctaa aaaggatgaa cgtggcgctc tcgtcaaccg tatgggggtt gacccgtggc
    33421 acaattggga ggcgcactat gaagtgttgc ctggtaaaga gaaggtcgtc tctgaactga
    33481 aacaactggc tgaaaaagcc gaccacatct atctcgcaac cgaccttgac cgcgaagggg
    33541 aagccattgc atggcacctg cgggaagtga ttggtggtga tgatgcgcgc tatagccgag
    33601 tggtgtttaa cgaaattact aaaaacgcga tccgccaggc atttaacaaa ccgggtgagc
    33661 tgaatattga tcgtgttaat gcccagcagg cgcgtcgctt tatggaccgc gtggtggggt
    33721 atatggtttc gccgcttcta tggaaaaaga tcgctcgtgg tctgtctgcc ggtcgtgtgc
    33781 agtcggtggc ggtccgcctg gtggtcgagc gtgagcgtga aattaaagcg ttcgtaccgg
    33841 aagagttctg ggaagtcgat gccagcacga ccacgccatc tggtgaagcg ttggcgttgc
    33901 aggtgactca tcagaacgac aaaccgttcc gtccggtcaa caaagaacaa actcaggctg
    33961 cggtaagtct gctggaaaaa gcgcgctaca gcgtgctgga acgtgaagac aaaccgacaa
    34021 ccagtaaacc tggcgctcct tttattacct ctacgctgca acaagctgcc agcacccgtc
    34081 ttggttttgg cgtgaaaaaa accatgatga tggcgcagcg tttgtatgaa gcaggctata
    34141 tcacttacat gcgtaccgac tccaccaacc tgagtcagga cgcggtaaat atggttcgcg
    34201 gttatatcag cgataatttt ggtaagaaat atctgccgga aagtccgaat cagtacgcca
    34261 gcaaagaaaa ctcacaggaa gcgcacgaag cgattcgtcc ttctgacgtc aatgtgatgg
    34321 cggaatcgtt gaaggatatg gaagcagatg cgcagaaact gtaccagtta atctggcgtc
    34381 agttcgtagc ctgccagatg accccagcga aatatgactc cacgacgctg accgttggtg
    34441 cgggcgattt ccgcctgaaa gcacgcggtc gtattttgcg ctttgatggc tggacgaaag
    34501 tgatgcctgc actgcgtaaa ggcgatgaag atcgtatctt accggcagtc gataaaggcg
    34561 atgctctgac gctcgttgaa ctgacaccag cccagcactt taccaagcct ccagcccgtt
    34621 tcagtgaagc atcgctggtc aaagagctgg aaaaacgcgg tatcggtcgt ccttctacct
    34681 atgcgtcgat catttcgacc attcaggatc gtggctatgt gcgagtagaa aatcgtcgtt
    34741 tctatgcgga aaaaatgggc gaaatcgtca ccgatcgcct ggaagagaat ttccgcgagt
    34801 taatgaacta cgacttcacc gcgcagatgg aaaacagcct cgaccaggtg gcaaatcacg
    34861 aagcagagtg gaaagctgta ctggatcact tcttctcgga tttcacccag cagttagata
    34921 aagctgaaaa agatccggaa gagggtggta tgcgcccgaa ccagatggtt ctgactagca
    34981 ttgactgccc gacttgtggt cgcaaaatgg ggattcgcac agcgagcacc ggggtattcc
    35041 ttggctgttc tggctatgcg ctgccgccga aagagcgttg caaaaccacc attaacctgg
    35101 tgccggaaaa cgaagtgctg aacgtgctgg aaggtgaaga cgctgaaacc aatgcgttgc
    35161 gcgcaaaacg tcgttgcccg aaatgtggca cggcgatgga cagctatctc atcgatccga
    35221 aacgtaagtt gcatgtctgt ggtaataacc caacctgcga cggttacgag atcgaagagg
    35281 gtgaattccg cattaaaggt tatgacggcc cgatcgttga gtgtgaaaaa tgtggctctg
    35341 aaatgcacct gaaaatgggg cgtttcggta aatacatggc ctgcaccaac gaagagtgta
    35401 aaaacacacg taagatttta cgtaacggcg aagtggcacc accgaaagaa gatccggtgc
    35461 cattaccgga gctgccgtgc gaaaaatcag atgcctattt cgtgttgcgt gacggtgctg
    35521 ccggtgtgtt cctggcggcc aataccttcc cgaaatcgcg tgaaacgcgt gcgcctctgg
    35581 tggaggagct ttatcgcttc cgcgaccgtc tgccggaaaa actgcgttat ctggccgatg
    35641 cgccacagca ggatccggaa ggtaataaga ccatggttcg ctttagccgt aaaaccaaac
    35701 agcaatatgt ctcttcggaa aaagacggaa aggcgactgg ttggtcagca ttttatgttg
    35761 atggcaaatg ggttgaaggg aaaaaataac ctttaattcc gtctggtttt tataaacaaa
    35821 gggtcgcgaa agcggccctt ttttattgca tattattttt tcttcacacc tatacactaa
    35881 ggctataaat gatatagtgg ttatagtgag cacctttttt attattaaat cgtattagtc
    35941 acccgcctgg tgtgacgaaa aaacgatgtt ctgatggcgt ctaagtggat ggtttaacat
    36001 gaaattacaa caacttcgct atattgttga ggtggtcaat cataacctga atgtctcatc
    36061 aacggcggaa ggactttaca catcacaacc cgggatcagt aaacaagtca gaatgctgga
    36121 agacgagctg ggcattcaaa ttttttcccg cagcggcaag cacctgacgc aggtaacgcc
    36181 agcaggacaa gaaataattc gtatcgctcg tgaagttctg tcgaaagtcg atgccataaa
    36241 atcggtcgcc ggagagcaca cctggccgga taaaggctcg ctgtatatcg ccaccacgca
    36301 tactcaggca cgctatgcgt taccaaacgt tatcaaaggc tttattgagc gttatcctcg
    36361 cgtttctttg catatgcacc agggctcgcc gacacaaatt gctgatgccg tctctaaagg
    36421 caatgccgat ttcgctatcg ccacggaagc gctgcatctg tatgaagatt tagtgatgct
    36481 accgtgctac cactggaatc gggctatagt agtcactccg gatcacccgc tggcaggtaa
    36541 aaaagccatt accattgaag aactggcgca atatccgttg gtgacatata ccttcggctt
    36601 taccggacgt tcagaactgg atactgcctt taaccgcgca gggttaacgc cgcgtatcgt
    36661 cttcacggca acggatgctg acgtcattaa aacttacgtc cggttagggc tgggggtagg
    36721 ggtcattgcc agcatggcgg tggatccggt cgccgatccc gaccttgtgc gtgttgatgc
    36781 tcacgacatc ttcagccaca gtacaaccaa aattggtttt cgccgtagta cttttttgcg
    36841 tagttatatg tatgatttca ttcagcgttt tgcaccgcat ttaacgcgtg atgtcgttga
    36901 tgcggctgtc gcattgcgct ctaatgaaga aattgaggtc atgtttaaag atataaaact
    36961 gccggaaaaa taatttcttg cgttattttc ggcacctttt atgtagcgaa ggtgccggaa
    37021 tatattctcc tttgttactt atttgtatcc ggtgcacaat atttttatcc ctcatattta
    37081 tagggtaaat tacctgtaaa gtagtgtcta attacacacc tagcaaactc cagttttcaa
    37141 atatctattt caggtcaaaa gattgaatta ttcactggag acgattcgta aattcgctgg
    37201 atttttgact aaagtttctt taggatttgt ctcatcgtat gattaattac accaatcgtt
    37261 caacgtcttg ctgataagtg atggcgatta tatgaggtta gcaatgcctt ctggaaatca
    37321 ggaaccccgc cgcaatcctg aattaaaacg taaagcctgg ctggcggttt ttcttggttc
    37381 tgcacttttc tgggtggttg tcgcactgct tatttggaaa ttgtggggat aactatggtc
    37441 ggtcaggagc aactggagtc gtcaccatta tgtcagcata gtgacaatga accagaagca
    37501 aaacgggaat gttccgtcgt tattccagac gactggcaac taacatcgca gcaacaagcc
    37561 tttatagaac tgtttgctga agatgatcag ccgaaacaat aattatcatc attcttatta
    37621 cccattttta atgaattaca gggcttttaa tacaccgcag caataacagg ttgagttatc
    37681 tcaacacaaa gtaataaccg ttaagggtgt agcctatgat caacacaaat atgaaatatt
    37741 ggtcctggat gggcgcattt tctctgtcga tgctcttctg ggccgaactc ctctggatca
    37801 ttactcactg atccctgacc ccgctgtttg ctttgcctca aggtttctcc tcttttatca
    37861 atttgggttg ttatcaaatc gttacgcgat gtttgtgtta tctttaatat tcaccctgaa
    37921 gagaatcagg gcttcgcaac cctgtcatta aggaggagct atgtcgtcaa ccctacgaga
    37981 agccagtaaa gacacgttgc aggccaaaga taaaacttac cactactaca gcctgccgct
    38041 tgctgccaaa tcactgggcg atatcacccg tctacccaag tcactcaaag ttttgctcga
    38101 aaacctgctg cgttggcagg atggtaaatc ggttaccgaa gaggatatcc acgcgctggc
    38161 gggatggctg aaaaatgccc atgctgaccg tgaaattgcc taccgcccgg caagagtgct
    38221 gatgcaggac tttaccggcg tacctgccgt tgttgatctg gcggcaatgc gcgaagcggt
    38281 taaacgcctc ggcggcgata ctgcaaaggt taacccactc tcaccggtcg acctggtcat
    38341 tgaccactcg gtgaccgtcg atcgttttgg tgatgatgag gcatttgaag aaaacgtacg
    38401 cctggaaatg gagcgcaacc acgaacgtta tgtgttcctg aaatggggaa agcaggcgtt
    38461 cagtcggttt agcgtcgtgc cgccaggcac aggcatttgc catcaggtta acctcgaata
    38521 tctcggcaaa gcagtgtgga gcgaattgca ggacggtgaa tggattgctt atccggatac
    38581 actcgttggt actgactcgc acaccaccat gatcaacggc cttggcgtgc tggggtgggg
    38641 cgttggtggc atcgaagcag aagccgcaat gttaggccag ccggtttcca tgcttatccc
    38701 ggacgtagtg ggctttaaac ttaccggaaa attacgtgaa ggtattaccg ccacagacct
    38761 ggttctcact gttacccaaa tgttgcgcaa acatggcgtg gtggggaaat tcgtcgaatt
    38821 ttatggtgat gggctggatt cactaccgct ggcggatcgc gccaccattg ccaatatgtc
    38881 accagaatat ggtgccacct gtggcttctt cccaatcgat gctgtcaccc tcgattacat
    38941 gcgtttaagc gggcgcagtg aagatcaggt cgagttggtc gaaaaatatg ccaaagcgca
    39001 gggcatgtgg cgtaatccgg gcgatgaacc aatttttacc agtgtgttag aactggatat
    39061 gaatgacgtt gaagcgagcc tggcagggcc aaaacgcccg caggatcgcg ttgcactgcc
    39121 cgatgtacca aaagcatttg ctgccagtag agaactggaa gtgaatgcca cgcataaaga
    39181 tcgcctgccg gtcgattatg ttatgaacgg acatcagtat cagttacctg atggcgctgt
    39241 ggtcattgct gcgataacgt cgtgcaccaa cacctctaac ccaagtgtgc tgatggccgc
    39301 aggcttgctg gcgaaaaaag ccgtaactct gggcctcaag cggcaaccgt gggtcaaagc
    39361 gtcgctggca ccgggttcga aagtcgtttc tgattatctg gcaaaagcga aactgacacc
    39421 gtatctcgat gaactgggat ttaaccttgt gggatacggt tgtaccacct gtattggtaa
    39481 ctctggaccg ttgcccgatc ctatcgaaac ggcaatcaaa aaaggcgatt taaccgtcgg
    39541 tgcagtgctg tccggcaacc gtaactttga aggccgtatc catccgctgg ttaaaaccaa
    39601 ctggctggcc tcgccgccgc tggtggttgc ctatgcgctg gcgggaaata tgaatatcaa
    39661 cctggcttct gagcctatcg gccatgatcg caaaggcgag ccggtatatc tgaaagatat
    39721 ctggccatcg gcacaagaaa ttgcccgtgc ggtagaccaa gtctccacag aaatgttccg
    39781 caaagagtac gcagaagttt ttgaaggcac agccgagtgg aaggaaatta acgtcacacg
    39841 atccgatacc tacggttggc aggaggattc aacctatatt cgcttatcgc ctttctttga
    39901 tgaaatgcag gcaacaccag cacctgtgga agatattcac ggtgcgcgga tccttgcaat
    39961 gctgggggat tcagtcacca ctgaccatat ctctccggcg ggcagtatta agcccgacag
    40021 cccggcgggt cgatatctac aaggtcgggg tgttgagcgg aaagacttta actcctatgg
    40081 ttcgcggcgt ggtaaccatg aagtgatgat gcgcggcacc ttcgccaata ttcgcatccg
    40141 taatgaaatg gtgcctggcg ttgaaggggg gatgacgcgc catttacctg acagcgatgt
    40201 cgtctctatt tatgatgccg caatacgcta taagcaggag caaacgccgc tggcggtgat
    40261 tgccgggaaa gagtatggat caggctccag ccgtgactgg gcggcaaaag gtccgcgtct
    40321 gcttggtatt cgtgttgtga ttgccgaatc gtttgaacga attcaccgtt cgaatttaat
    40381 tggcatgggc atcctgccgc tggaatttcc acaaggtgtg acgcgtaaaa cgttagagct
    40441 taccggggaa gagaagattg atattggtga tctgcaaaat ctacaacccg gcgcgacggt
    40501 tccggtgacg cttacgcgcg cggatggtag ccaggaagtc gtaccctgcc gttgtcgtat
    40561 cgacaccgcg acggagttga cctactacca gaacgacggc attttgcatt atgtcattcg
    40621 taatatgttg aagtaacgag tatttgcttg ccggttattt tgcttccggc aagcaaatta
    40681 attacacaat gcaagagggt tatttgttca gcaaatgacc cattttctcg gctttggtat
    40741 cgagataatg ttcgttattg gggttacgac ctacaatcaa cggtacgcgt tcaataatat
    40801 taatccctgc ttcggtcaga atttcgactt ttttcgggtt attggttaac aagcggactt
    40861 cattgacgcc aaggagtttg aacatatcag cgcaaagagt gaagtcgcgc tcatcagcgg
    40921 cgaagcctaa ctggtggtta gcctctacag tatcgtaacc ttgatcctgc agtgcgtaag
    40981 cgcggatttt attcagcaga ccaatgttac gaccttcctg acggtgatac agcaaaatac
    41041 cacggccttc ctcggcaatt tgcgtcaatg ccgcttcgag ctggaagcca caatcgcagc
    41101 gcaagctgaa cagggcgtca ccggtcagac attcggaatg gacgcgcgca agtaccgggg
    41161 tatgcccgga aatatcgcca tagactagcg cgacatgatc gtgtccggtt gccagttctt
    41221 caaatcccac catcaggaaa tcgccccatg gggttggcag tttggcttct gccacacgtt
    41281 taagctgcat gaaattctcc agataatgct ggttctgtat tggcttattt tgccataacg
    41341 agaaggggtt cacctaatca cgacgcgtcg atcgttcacg gaatggcaca aatctgtcaa
    41401 tttttcctgg aactggcatt ttcagttatg attgtgggac ttatcaaaaa ggagaggcca
    41461 tgcgttcgat tgccagacgt accgcagtgg gagctgcact attgcttgtc atgccagtag
    41521 ccgtatggct ttctggctgg cgttggcaac ctggagaaca aagttggtta ctaaaagcgg
    41581 ctttttgggt tactgaaact gtcacccagc cttggggcgt cattacacat ttgattttat
    41641 tcggctggtt tctctggtgt ctgcgttttc gcattaaggc tgccattatg ttatttgcca
    41701 ttctggcggc ggcaatcctt atgggacaag gcgttaaatc ctggatcaaa gacaaagtcc
    41761 aggaaccacg accttttgtt atctggctgg aaaaaacaca tcatattccg gttgatgagt
    41821 tctacacttt aaagcgagca gaacgcggaa atctggtgaa agaacagttg gctgaagaga
    41881 aaaatatccc acaatatttg cgttcacact ggcagaaaga gacggggttt gcctttcctt
    41941 ccggtcacac gatgtttgct gccagttggg cactgctggc cgttggtttg ctgtggccgc
    42001 gtcggcgaac gttaaccatt gctatcttgt tggtctgggc aacgggagtc atgggaagcc
    42061 gcctgctgct cgggatgcat tggccacgcg atctggttgt agctacgatg atttcgtggg
    42121 cgctggtggc ggtggcaacg tggcttgcgc aacgaatttg tgggccatta acaccacctg
    42181 cggaagaaaa tcgcgaaatt gcacaacgag aacaagaaag ttaacgctgg ttgattttcc
    42241 gaatttagcc cttaaatcat caacaatgcg cgtggatgcc atttcgcaga cggcgcgaaa
    42301 atggtacttt aaagggctat tgcggtaagt tgaccataat ttattcgctc taaccacata
    42361 acgggaagta atgtgaaata tttactcatt ttcttactgg tgttagcgat cttcgtgatt
    42421 tcggtcacgt tgggtgcgca gaacgatcaa caggtgacgt ttaattatct gttagcgcaa
    42481 ggggagtacc gtatttccac attgctggcg gtattgtttg ctgcggggtt tgctatcggt
    42541 tggttgattt gtggcctgtt ctggctgcga gttcgtgttt ccctggcgcg cgctgaacgt
    42601 aaaataaagc gactggaaaa ccagctttca cccgcgactg acgtggctgt agcgccgcac
    42661 tcgtctgcgt cgaaggaata actttctatg ctggagttgt tgtttctgct tttgcctgta
    42721 gccgctgcct atggctggta tatgggccgc agaagtgcgc aacaaaacaa gcaagatgaa
    42781 gccaaccgct tgtcgcgtga ttacgtagcg ggggttaact tcctgcttag taatcaacag
    42841 gataaagcgg tagacctgtt tctcgatatg cttaaagagg atacaggtac cgttgaagcc
    42901 caccttacgc tcggaaacct gttccgttcg cgtggcgaag ttgatcgcgc tattcgcatc
    42961 catcagaccc taatggaaag cgcctcgctg acctatgaac agcggctttt ggcgattcaa
    43021 caattggggc gtgattacat ggctgccggg ttatacgacc gcgcggaaga catgttcaat
    43081 cagctgacag atgaaactga cttccgcatt ggcgcgttgc aacaactgct acaaatttac
    43141 caggctacca gcgagtggca gaaagcaatt gatgttgccg aacgcctggt gaagctgggt
    43201 aaagataaac agcgcgtcga aattgcccat ttctactgtg agttagctct gcagcatatg
    43261 gccagcgacg atctcgatcg tgccatgact ttgctaaaaa aaggtgcggc ggcagataaa
    43321 aacagcgccc gcgtatccat catgatggga cgcgtgttta tggcgaaagg agaatacgcc
    43381 aaagccgtcg aaagtttgca acgtgtcata tcccaggaca gagaactggt cagcgaaacg
    43441 ctggaaatgc tgcaaacatg ctatcagcag ttgggtaaaa ctgccgaatg ggcagaattc
    43501 ctgcagcgcg cggtggaaga gaacaccggt gccgatgctg aattgatgct tgcggatatc
    43561 atcgaagcgc gcgacggtag tgaggccgca caggtctata ttacgcgcca gcttcagcgt
    43621 catccgacca tgcgtgtgtt ccataagtta atggattacc acttaaatga agcagaagaa
    43681 gggcgtgcca aagagagtct gatggtgctg cgtgacatgg ttggcgagaa ggtacgtagt
    43741 aagccccgtt atcgctgcca gaaatgtggt tttaccgcat acaccctcta ctggcattgt
    43801 ccgtcttgtc gggcctggtc aaccattaaa ccaattcgcg gtcttgatgg cctgtaattt
    43861 ttaaaaaaat ccgactttag ttacaacata ctaattattg atgttctatt gtgctccggc
    43921 accgacggcg cggaaaagcc tgccagagga gaaatcgcaa ctgttaattt tttatttcca
    43981 cgggtagaat gctcgccgtt tacctgtttc gcgccacttc cggtgcccat catcaagaag
    44041 gtctggtcat gacgttaact gcttcatctt cttcccgcgc tgttacgaat tctcctgtag
    44101 ttgttgccct tgattatcat aatcgcgatg ccgcgatggc ctttgtcgac aagatcgacc
    44161 cacgcgattg tcgtctgaag gtcggcaaag agatgtttac attgtttggg ccacagtttg
    44221 tgagcgaact tcaacagcgt ggttttgata tctttcttga cctgaaattc cacgatattc
    44281 ccaacactgc agcgcacgct gtcgctgttg cggctgactt aggcgtgtgg atggtgaatg
    44341 ttcatgcctc cggtggggcg cgtatgatgg ccgcagcgcg tgaggcactg gttccgtttg
    44401 gcaaagatgc accgcttttg attgctgtga cagtgttgac cagcatggaa gccagcgacc
    44461 tggccgatct tggcgtgaca ctgtcacctg cagattatgc agaacgtctg gcggcactga
    44521 cgcaaaaatg tggccttgat ggtgtggtgt gttctgctca ggaagctgtg cgctttaaac
    44581 aggtattcgg ccaggagttc aaactggtta cgccgggcat tcgtccgcag gggagtgatg
    44641 ctggtgacca gcgccgcatt atgacgccag aacaggcgtt gtcggctggt gttgattata
    44701 tggtgattgg tcgcccggta acgcaatcgg tagatccagc gcagacgctg aaagcgatca
    44761 acgcctcttt acagcggagt gcatgatgag tgattccaac agccgtctgg tctactcaac
    44821 ggaaaccgga cgtattgatg agccaaaagc ggcccctgca cgccctaaag gcgacggtgt
    44881 ggtgcgtatt cagcgtcaga ccagtggacg taaaggtaag ggcgtttgcc tgattaccgg
    44941 tgtcgatctc gatgatgccg aactgacgaa actggcagcg gaactgaaga aaaaatgcgg
    45001 ctgcggcgga gcagttaaag atggaattat tgaaatccag ggcgataagc gtgatttatt
    45061 aaaatcactg cttgaagcga aagggatgaa ggtaaaactc gcaggcggct aacataaaaa
    45121 gccacggata tatccgtggc tttcgaatat tttactgtgt gtattattca gttatttcgt
    45181 actgaacaga atcaagcgta ttataattgc gagtcgataa tcgacattta tttacgatta
    45241 tttaccgacc tggtgaccaa taacaccacc gacggctgca ccacctaatg tacccaacgt
    45301 actgccatcg gtcagtactg caccgcctaa tgcccctgca cctgcaccga ttgcggtgtt
    45361 gcggtcccgt ttagaccagt tagaacaggc actcagagac attgccaggg taattgccag
    45421 aacagccgcg gtcatttttt tgctcgttac aaacataata ctctctcctg aatttatgat
    45481 tcacggaagt aagctctcta taactataat agctaagaat aagcctggtg aaattattcc
    45541 gtgaaatccg ctcgcgcgca gggttatatc acgcaggtga taatgacttc ctgttatatc
    45601 gctgataata attttatatc ttgagagtgt taataacagg taaatagtct taattatcaa
    45661 ccaggaatca tcttagagcg gatgatttgc caaactgcaa atcatccgta gagaagggaa
    45721 atggttaaat gtcaacgacg tgaacggtga gtttactttg ttcaagatgc atcagatcgc
    45781 tggcgcgtat tttcgaatcg gtaatcacgc gattgaatcg ctcaacgggg ccgatggagt
    45841 atgaatgtac agcaccaaat ttcgagctgt cagtcaggac tatcgcctcg cactcttttt
    45901 ccagcacggc attgaccaca tcggtacgca tcatatcgcg accggtaaac ccagtttcag
    45961 gttgccagcc atcaatacca ataaatgctt tgctgaaatg cacttgttgg atgcactggc
    46021 gtgtcaaagg gccaaccata ctttcgcttt ttttctggta cacgccaccg agcaaaatga
    46081 cttcacaagg cgcgtctttc agcaaatgcg cgatgtagct gctgaccgtg atgatagtga
    46141 catttttctt ctgctcgccc agagtcctgg cgagcagggc attgctgcta ccattttcga
    46201 taaagatggt ttcgcccggt tgaaccagtg acgcggcaaa ctcggcgagt tcacgcttca
    46261 gcgtatagtt gctcatcata cgggtttcga cgtcatcact atcaagcgaa actgcaaaac
    46321 catgtgcacg tcggaggtaa ctcagttttt cgagggtgtt gagatcctgg cgaatggtaa
    46381 cttcagaaac tccagtggct tttgccagat cggttacgct aacctgaccc tggtcaatga
    46441 ccatctgtag aatagtttgt tgtcgggaat tcatcgctgt aattcttata acgttataat
    46501 actaaataaa aaaatactga cgcgccgggc atcctcaaat aggaaaacct gaaggcacga
    46561 ttgttagatc tcccacggtg ccttcgggat gtctggcgta gattcagcaa cctggcgcaa
    46621 gagttctgct ttaagtatct cgagattatg gatcgcagag tggtaatcgc ctgccacaag
    46681 gatatctaac acaatatcaa tacgttcggc aacgcgctgg gcatcaaatt cagacatcaa
    46741 ggacatcctt atagcaaaag gggaaaaatg aataatgcaa aaattgccgc taaaagagaa
    46801 gtgtttaaca gcaacggcta attatcatcc gggaatacga tatataatga cggaatatag
    46861 cgctaagtat atatattcat ctacttatgt gcgcttcagg tagcgtttat accagcgttc
    46921 gaaggcaacg gcgggcatcg gtttggcaaa caaaaatcct tgtcgctcat tgatcccgtt
    46981 cttggttaaa aaagcatctt cctttgcact ttctacacct tcggcgatca cctgaagatt
    47041 caatgcctgg gccacagcga cgatcgcccg gaccagtgat tgcgagacag gctgtttgtg
    47101 aatatcccga acaaaaacct ggtcaagttt gatggcatcg atcggaaagc gcgccagttg
    47161 cgaaagtgaa gagtagccgg taccaaaatc gtccagatga acttgcgcac ctaatcggct
    47221 aaattgttga ataacagaca gtgccagttc atcattctca atcaggcagc tctccgtcag
    47281 ttcaacatct atagggcagt attcaaaatt gagttcctgg agaacctgtt tcagggcggt
    47341 gaaaatggtt tgatcggcga gctgacgtgc agaaatattt accgccactc gcaggttaat
    47401 gcctttatcc cgccacttcg ctacctggcg tacgacatcg agaatcaccc aacgtcctaa
    47461 aggcacaatt agccctgatt cttcggcgta ggaaatgaag tccaacggtg gaatcaaccc
    47521 acgttcaggt gactgccaac gtactagtgc ttccagactg cgcacttcgc cacgccaggt
    47581 gattttcggt tgatagtgaa taaccaactg atcgttttcc agtgctttac gcaagttggt
    47641 atccagccag agatattcaa atacccgctg attcatttct ggggtaaaaa cgcaaaattg
    47701 tcctcgtccg ccttccttcg ctgtgtacat tgctgtgtcg gcgtgacgaa taatagccgc
    47761 gctgtccgaa ccatgttcgg gagagagtga aatacctact gaacagctgg tataaacttc
    47821 aattaaacca atgcgaaagg ggagtcgtaa gcgggtcaaa attcgtgatg ccaatgcttc
    47881 cagcgcgctt tgtgaggtat tggatgccag taccagaaac tcatcgccac ctggacgcgc
    47941 caacacctgg tcatgttcga gacagcttaa aatagccaat gacacgtcgc gtaataactg
    48001 gtcaccaaac aaatgcccat aggcgtcgtt gaccttttta aaattatcca aatcaagata
    48061 cacaacccca actttattgt tatctgcctg attaatagcg tgatcgatta actcctgcat
    48121 cgcgttacga ttcggcagtc cggtgatact gtcggtattt gccagaatac gcagtcgctc
    48181 ctgagcgcgg cgctcttcgg taatgtcggt gccggaacag attaaaaaaa tctcgttttt
    48241 gccactgccg ctgtggacaa atttattgcg aaacagaaac agccgctggc ctttacgtgt
    48301 tggtatccat agttcgactt catatgcatt gccgcttcga aaaaatacac ggttattgcg
    48361 cctggatgcc gcagcttcac gacgactcat aaacagttta aacacgcttt gcccaatgac
    48421 gtcgtgttct ttcaagcctg tgtaatcttc acataaccga ttaaagcgct ggatattccc
    48481 ccggctgtcg agaatcacta ttgcggaatg tgcttcagaa actacctgct cagcaaagga
    48541 aagcccatgt gataaatcac gagcaaccgc cggtgtgtca tgccatgcag acgcgttgcc
    48601 tccccattct cgtttattga tttttcgtcc taccagatgc acagaaagat cgctttcatc
    48661 gacggtcaga ctcatcatca ggctggaggt gatgaccgtc atttccctga tgcgagcagc
    48721 ctgttcggca gatagttgca aaatacgatc gggttctgtg atttcggtgg tagaaaagcg
    48781 caaaacatcg ctgctttccg tcaaccgcca gtatggattg tgcgatccga gaaagttgta
    48841 caacgttgtg gactccctaa cggttttcat gatgcatatc ctccggttaa cagcggagtc
    48901 cgcagttaaa taaaaggaac aacatgactt acatgaaatt aacggcggct aaacacattg
    48961 tttttttata tatttatttg taatccagtt ttggaaaaac gccagttttc aaacgaaagt
    49021 cagttaaaaa tctgccggga tatgacgaag gttgagcggg gaaataaacg gcccatccat
    49081 gaggaatggg ccgtgaaagg agattacgcg actgggcgcg caataatgct gcgggtttcc
    49141 atgcggactt cggcaatggt gacgtcaata acgtcagtca ctttgtaagc cgtttcacct
    49201 ttaatttgta cagtgccgtt ttcctggctg caaaccagtt catcgcgcac agcgtgtaag
    49261 aaaggtgctg gaataaaggc gatagcgccg ttatcaacca aacgaacacg catgccgcca
    49321 cggctgatat cgacaatttc cgccgcgaaa cgggtgtcgg tcccggcttt gtctttcagg
    49381 aagcgtgcgt ataaccagtc gccaacatca cgttctgcca tccggttgag acggcgacgc
    49441 tcggccattt ggacagtgat ttcatcctgt ggacgcgtcg cagtttcgcc tttgataacc
    49501 gctttcagca gacggtggtt gatcatgtcg ccatatttac ggatcggcga agtccaggtg
    49561 gcgtatgctt ccagaccgag gccaaagtga ggaccgggtt cagtgctaat ttcagcaaat
    49621 gactggaagc gacgaatgcg gctgtcgagg aaaccagttg gttgcgcatc cagttcacga
    49681 cgcagtttgc agaaaccgtc cagcgtgagc acttcttcag catcgacatg cagaccgtgc
    49741 gttttcagca acgctgccaa cgcgtcggca ttcgccggat caaagcccat atgcacgtta
    49801 tagatgccaa aaccgagctt atcgcgcagt acgcgggccg cacaaatgtt agcggcaatc
    49861 atcgcttctt cgacgatacg gttggcaatg cgacgaggct cggcgacgat atccagcact
    49921 tcaccttttt caccgagaat aaagcggtaa tccgggcgat ctttaaacac cagtgcgtgg
    49981 ttatgacgcc actcgccgcg gcgttggcaa atttgtgcta gcaaacggac ttgttcggca
    50041 attgcttcgc tttcaggctg ccagtcaccg gtattttcca gccagtcaga aacctggtca
    50101 tacaccagct tcgctttgga ttcgatggtg gcggcaaaga attcgatatt atcttcaatg
    50161 gtgccatcag tggagagcgt catgcggcat gccagtaccg ggcggacttc attggcgcgc
    50221 agtgagcaga gatcgtcaga aagctcgcga ggcagcatag ggatgttgaa gccaggcaga
    50281 tagttggtga atgcgcgaat tttcgcggct ttgtccaact tgctgccttc agcaatccac
    50341 gcggttggat cggcaatcgc cacaatcagc tgaagtttgc catccggtaa cgccttggcg
    50401 aaaagggcgt catccatatc ttctgtgctg gcactgtcga tggtgacaaa atccagcgca
    50461 gtcagatctt cacgaaccag accttcatcg agcatttcgg tagcgacgcc gtctggtgct
    50521 tctttttcca gattatgacg cgcaagggta acccaccacg gtacaaagtg atcgtcacca
    50581 aaagtgatgt attgtgtcag ttcagcatag aaagaacgat cgcctttcag cggatgacgg
    50641 cgcatttcgg caaccgccca gtcgccttct ttaaactcgt ggttcaggcc acgggctgcg
    50701 cggcaaggaa tggcgtcttt taagagtgga tgatcaggaa cgatggccag acggtcattt
    50761 ttgccctgaa ctttacccac gaaacgagtc aggaacggtt caaccagttc ttctggctct
    50821 gcggattcac gttctttttc actgtggatc accgcgataa ttcggtcgcc atgcatgact
    50881 tttttcatct gcggcggcgg aatgaaataa cttttttgcg cgtcgacttc caggaagcca
    50941 aagccttttt ctgtggcttt taccacccct tcagcgcgtg gcgtctggga atgcagttgc
    51001 tgtttaagct gcgctagcag cgggttgtcc tgaaacataa ttgtctattt tggtggccat
    51061 tagagcggct gacagtttta cgcgaatctg tctgacgcgg caaggttaat atgtctcacc
    51121 caacgcgatt tttaagcgat ttatccagcc acacagcccg ctccatacca gcagattaat
    51181 aatctgcgtt gatgattttt cgtgttcgag taatgggctg aactgggcgg cgctaaagcg
    51241 atcgggtgct cgggtcagcc attgtatcgc ctgaacggta gcgcgctcaa cagaatgacg
    51301 gtcagcccat cggttaattt cattttcgcc cttgcgcaga aggaggctga tttttttata
    51361 ttcatttgat gaattcatac aggcggcaaa acaagcagga ctgccattta tccgcgcgca
    51421 taacaacgcc gcaagcaggt tacccggctg tgttttaacc cgagtgctgt ttaacaacat
    51481 ttcgagaagg gaaagtgaaa tagggtggag agagagtgtt tcggtaagtt ggcaaagttc
    51541 tgcagtcgaa ctttttgtgt actcttcagg aagtttttcc acctcatagc tgcttcgcca
    51601 gcgtaatgat tcatcagcaa acagtgacgc gtcggcgtag ttttgtatct ccagcccggg
    51661 taaccagcgt accggatgcc cgagataagc ctgaaatgtc gcaattgtcc gcgcctgaaa
    51721 gccaataaaa ccaatgattt ggttaatgat cacacagtca caggcagtaa gaccaacgtc
    51781 attcagatgc tggcgcgatc ggttgtcgat aatagaaggc gagctagcca gttggcgggc
    51841 atattgcgtg atttgtgcca accgatgatt actttccctg gaggaatcag gcccggggag
    51901 cggcgtaagt cgcgccgaat agtagttaca taaccgttgg acgccgcaaa cctgagcaac
    51961 cgtcagggcc gtcgacaagc gttcataagc ggtgaaggtc tgtgaacggg tcacggtaag
    52021 cgggtcaggg aacaattcta ctgcaagaag gcgcgcaggg accagccatg aaaggaaggg
    52081 ggccagcgtt tcttcatcaa ggatcacgtc gagcagaaag ggatcgctga cctttgcggc
    52141 ttccgggacc agaggcagaa cgtcatactc cgcagtactg gattgcgttt catgatacca
    52201 gtggcttttg ccggtgatgt ggcgttgttc catgggcgct ccttggtcgt aaaggaaatc
    52261 gttatcctga cgcaaggcgg gaaggggaga aagacggatc ggcgataaca aatatcagaa
    52321 aggtataaca gagataacgg gcggcagaac gccgcccatc tttaccaaca gaacgattat
    52381 ttcagttcga gttcgttcat tgcagcgatg ctgaaaccgc cgtcaacgtg gaccacttca
    52441 ccggagatac cggcagagag atcggagcac aggaatgccg cggaattacc cacatcttca
    52501 atagtaacgg tacggcgaat cggggtaacg gcttcgcaat gagccagcat tttgcggaag
    52561 tctttgatac cggaagccgc cagagtacgg atcggacccg cagagatggc gttaacacgc
    52621 acaccttccg gacccatcgc gttcgccata tagcgcacgt tcgcttccag agacgctttt
    52681 gccagaccca taacgttgta gttcgggata gcgcgctcag cgccaaggta ggaaagggtc
    52741 agcagggcag aacccggatt cagcatggag cggcaagctt ttgccattgc aacgaagctg
    52801 taggagctga tgtcgtgggc aattttgaag ccttcacgag taacggcgtt aacatagtca
    52861 ccatccagct gatcgccagg tgcaaaacca atagagtgta cgaaaccgtc aaatttcggc
    52921 caaactttcc ccaattcagc gaacatggtg tcgatgctgg tatcttcggc aacatcgcac
    52981 tgcagaacga tgtcagaacc caattgagcg gcaaattctt ctacgcggcc tttcagtttg
    53041 tcgttctggt aggtgaatgc cagttcagct ccttcgcggt gcatcgcctg agcgataccg
    53101 taggcgatgg acagtttgct ggcaacaccg gttaccagaa tgcgcttacc ggaaagaaaa
    53161 cccatagctt taatccttat tgttgatgct tgttgtgcct gaaaatcagg cgattcgtcg
    53221 ttttagtaaa cagtacgaac agataaacag ttattataat caacctggct gtgggtggct
    53281 atagttgcgc tttctgtagc ggagagaagc tgcggtacag agcattttgc gttatctact
    53341 cctgacgcct gcaaggggga ggttggggaa atatcttata gaatctgact ttgaaactat
    53401 tcgtattagc tccgataagt ttgatgatga aaacagaact ggcgacaaca agtcgctgtc
    53461 tttctgatga attgactcca acttaatatt gtcaactatc ttcacagaat ctccacaatc
    53521 tcttcattat cagaatatgc atcatgaata agataaaaat actttccatt actttggaga
    53581 aacgtataat tagcaaaaat gagaatgatc attctcacca agtgataggc caggagacag
    53641 aaatgaccag caagctggag atacgccaca aacagcgtca ggatgaaatc attaacgccg
    53701 cccgtcggtg ttttcgtcgc tgcggatttc atgccgccag tatgtcgcaa attgccagcg
    53761 aagctcagct tagcgtgggt cagatatatc gttacttcgc caacaaagac gccattattg
    53821 aagagatggt tcgccgtatc atcgactttc gtattgccca aatggacatt gacgcgcgta
    53881 ccgatcactt cccggaagtc cttgccttgc gtaaatcctt aaatgaggac gacgacgcgc
    53941 tgatgctgga agtggctgca gaagccacac gcaacccgcg ggttatggca atgcttgaag
    54001 aagctgacgc cagaatgttc gctaacggtt gtgcacatat gaaaagaatg catccccatc
    54061 ttagcgacga gcacattcgt tgctgcgttg aagtgtttgc taccatgatg gaaggtacgg
    54121 tttatcgtcg tttaacaccg caaaaaagcg atccacaaca tctgcaggaa atatatcagg
    54181 atattgttag tatgttgata aataaataag aacagaatta accaatagcg ggggatatta
    54241 atccccctta tttcattgcg aaattcattt cgcgatgcgg gttcgttata actgaatatc
    54301 cctgacatat cctcattaat ttcaatgagg agggtatttc tcaccctgga gaaaataatg
    54361 aagtatatag caacatctgt agtggcaatg ctgctcttat cgggttgtga taatacgcaa
    54421 agtaacaatt catccccgtc agaaacagaa gtcggcgttg ttacgctcaa atctcaaccg
    54481 gtttcggtag tcagtgaatt aaccggacgc accagtgctg cgctcagtgc tgaagtacgt
    54541 ccgcaggttg ggggaattat ccagaaacgc ttatttaagg aaggtgatct ggtcaaggct
    54601 ggacagcctc tctaccagat tgatgcggcc agttatcagg ctgcatggaa tgaagcccgg
    54661 gcagcattac aacaagcaca ggcactggta aaagccgatt gccagaaagc gcagcgttat
    54721 gcccgactgg tgaaagagaa cggtgtttca caacaggatg ctgatgatgc tcagtctacc
    54781 tgtgcacaag ataaagccag tgtagaggcg aaaaaagccg cactggaaac tgcgcgcatt
    54841 aatcttgact ggaccacggt aaccgcaccg atttcggggc gtattggtat ttcgtcggta
    54901 acccctggcg cactggtgac cgcgtcgcag gatacagcgt taacgactat tcgtggtctg
    54961 gatacaatgt atgtcgacct cactcgctcc agtgtcgatt tattacgtct gcgtaaacag
    55021 tcactggcga ccaacagtga caccatgagc gtctcactta ttctggaaga tggcacaacc
    55081 tacagcgaaa aagggcgtct ggaactcacc gaagtcgcgg tggatgagtc taccggttcg
    55141 gtgacattac gggcaatttt ccccaatcca caacagcagt tattaccggg aatgtttgtt
    55201 cgcgctcgtg tcgatgaagg cgtgatggaa gacgctattc tcgcgcctca acagggcgtt
    55261 acgcgcgatg ctaaaggcaa tgcaactgcg ctggtggtga ataaagacaa taaagtagag
    55321 cagcgaacgc tcgaaacggg agaaacgtat ggtgataaat ggctggtgct gaacggcctg
    55381 cacaacggcg accgactgat tgttgaaggt tctgccaaag tcacttcagg gcagaccgtc
    55441 aaggctgttg aagttcaggc taatggaggc aacgcctgat gttttcgcgc ttttttgttc
    55501 gccgcccggt ctttgcctgg gttatcgcca ttttgattat gttggcgggg attctggcta
    55561 ttcgcacatt gcccgtcgcg caatatcctg acgttgcacc gccgaccatt aaaatttcag
    55621 ccacttatac tggtgcttct gccgaaacgc tggagaacag cgtgactcag gttatcgagc
    55681 aacaactcac tgggcttgat aatttactct atttcagctc aaccagtagc tctgatggtt
    55741 cggtcagtat taatgtgacc tttgaacaag gtaccgatcc agatactgca caggttcagg
    55801 tgcagaataa aattcagcag gcggagtcgc gcctacccag cgaagtgcag caaacgggtg
    55861 ttacggtgga gaaatcacaa agcaactttt tgctgattgc tgccgtgtat gacaccactg
    55921 acaaagcctc cagttcggat atcgccgact ggctggtcag taacgttcag gaccccctgg
    55981 cgcgtgttga aggtgtgggg agtctgcaag tctttggcgc ggaatacgcc atgcgcatct
    56041 ggctcgaccc ggccaaactg gcgtcttact cgctgatgcc ttcagacgtg caaagtgcta
    56101 ttgaagcgca aaacgtgcag gttactgccg ggaaaatagg ggcattgcct tcaccgaata
    56161 ctcagcaact gaccgcaacg gtacgtgcgc agtctcgctt gcagacggtg gatcagttca
    56221 aaaatattat cgtgaaaagc cagtcagacg gcgcagttgt tcgtataaaa gatgtggctc
    56281 gcgttgagat gggcagtgaa gattataccg ctatcggcaa acttaacggt cacccatctg
    56341 ccggggttgc tgtaatgctt tcgcccggtg cgaatgcgct gaatacggcg acgctggtca
    56401 aggataagat tgccgaattc cagcggaaca tgccgcaggg atacgacatc gcatacccga
    56461 aagacagcac tgaatttatt aaaatctccg tagaggacgt aattcagacg ctgtttgaag
    56521 ccatcgtatt ggtggtttgc gtgatgtatt tattcctgca aaacctgcgt gccacactga
    56581 ttccggcgtt agctgtcccc gtcgttttgc tgggcacatt cggcgttctt gcgttgtttg
    56641 gctattcgat taataccctg acgttgtttg caatggtgct ggcgatcggc ttgctggtgg
    56701 acgatgccat tgtggtggtg gagaacgtcg aacgcattat gcgtgacgaa gggttgcccg
    56761 cgcgtgaagc cacggaaaaa tcaatgggcg agatttctgg cgcactggtt gccattgcgc
    56821 tggtgttgtc agccgtattc ctgccgatgg ccttctttgg gggatctacg ggggtaattt
    56881 atcgtcagtt ctccatcacc atcatctccg caatgctgct ttccgtggtg gtggcattga
    56941 ccttgactcc cgccctgtgc ggttccgtcc tccagcatgt tccgccacat aaaaaaggct
    57001 ttttcggcgc atttaaccgc ttttaccgcc gtactgaaga taaatatcag cgaggcgtaa
    57061 tttatgtcct gcgccgtgca gcccgaacga tggggcttta tgtcgtgctg ggtggtggaa
    57121 tggccctgat gatgtggaaa ctgccgggca gtttcttacc cactgaagac cagggcgaaa
    57181 tcatggtgca gtacacgctg ccggccggtg caaccgctgc ccgtacagca gaagtgaatc
    57241 gccagattgt tgactggttc ctgattaacg agaaagcaaa taccgatgtc atctttaccg
    57301 ttgatggttt cagttttagc ggcagcggac agaacaccgg gatggcgttt gtttcgttga
    57361 aaaactggtc tcaacgtaaa ggggcagaaa acaccgccca ggctatcgcc ctacgggcaa
    57421 ccaaagagct gggcacaatt cgtgatgcca cggtatttgc gatgacgccg ccagccgttg
    57481 atgggctggg gcaaagcaat ggttttacgt ttgaattgtt agctaacggt ggaaccgatc
    57541 gtgaaacact gctgcaaatg cgtaatcagt tgatagaaaa agcgaatcaa agtccggagt
    57601 tacattctgt acgcgccaat gatttgccac aaatgccgca attgcaggta gatattgata
    57661 gtaataaagc ggtgtcatta gggttgagtt tgaatgatgt caccgacacc ctgtccagtg
    57721 cgtggggcgg tacttatgtg aatgacttta ttgaccgtgg tcgggtgaaa aaagtctaca
    57781 ttcagggcga cagcgaattt cgttccgcgc cgtcagactt aggtaaatgg tttgtgcgcg
    57841 gtagcgataa cgccatgaca ccattctctg cttttgcgac cacccgctgg ctgtatggac
    57901 cggaaagact ggtgcgctac aacggctcgg cagcctatga aattcagggc gaaaacgcga
    57961 ctggctttag ttccggcgat gcaatgacaa aaatggagga actggcaaac agtcttcctg
    58021 cgggaacaac ctgggcctgg agtggtttgt cattgcagga gaaactggcc agcggtcagg
    58081 cattaagtct gtatgcagtt tctattctgg tggttttcct ctgccttgca gcactgtacg
    58141 aaagctggtc agtcccgttc tcggtcattc tggtgatccc tctggggctt cttggcgcgg
    58201 cgctggcggc ctggatgcgt gatttaaaca acgacgttta cttccaggta gcgctattaa
    58261 ctactatcgg cttgtcgtcg aaaaacgcca ttctgattgt ggaatttgct gaagcggcgg
    58321 ttgcagaggg ctattccttg agtcgtgcgg cattacgcgc ggcgcagact cgtttacgcc
    58381 caatcatcat gacctcgctg gcgtttattg cgggggtaat gccgctggcg atagcaaccg
    58441 gcgcaggggc gaacagccgc atcgccattg gtacgggcat tattggcggc acgctgaccg
    58501 ctacattact ggccattttc ttcgttcctc tgttttttgt actggtgaaa cgtttgtttg
    58561 ccggtaaacc gcgccgtcag gagtaagtta tgttgcgtcg ttctcttatt tttctggtgt
    58621 tgttgagtgc gggatgtgtc tcgctcgatc ctcactacag tacgcctgaa tccccaatcc
    58681 cggcgacctt gccgggagca cagggccagg ggaaggcaat cagccatgac tggcagcagg
    58741 tgatccacga tccccgattg cagcaggtag taaccattgc actgaacagt aaccgcgatg
    58801 tgcaaaaagc gattgctgat attgactcag cacgggcgct ctatgggcaa actaatgcgt
    58861 cgttatttcc gacggtgaat gcggccttaa gttcgactcg cagtcgttcg ctggcgaatg
    58921 gtacgggaac aactgccgag gctgacggca cggtgtccag ttatacgtta gatctgtttg
    58981 gtcgtaatca gagtttatct cgggcggcgc gggaaacctg gcttgccagt gaatttacag
    59041 cccagaacac tcgtttaacg ctgattgctg aaatcagcac cgcctggctg acactggcgg
    59101 ctgataacag caacctggcc ctggcaaaag aaacgatgac cagtgcagaa aactcattga
    59161 aaattatcca gcgccagcaa caggttggca cggcggcagc gacagatgtc agtgaggcga
    59221 tgagtgttta tcagcaagcg cgcgccagcg ttgccagcta tcagacacaa gtgatgcagg
    59281 ataaaaatgc gctgaactta ctggcaggta caacgcttgc ggaaaatctg ctaccgggaa
    59341 cactggaaag cctaccggaa caaatgataa gtctggttcc tgcgggagta tcatctgatg
    59401 ttctgctgcg tcgcccggat atccaggaag cggaacataa tttgaagagc gccaatgcag
    59461 atattggcgc ggcgcgggct aactttttcc cgacgatttc gctgactgcc agtgccggtg
    59521 ttggcagtga cgcattgtcg tcgttgttca gccacggaat gcagatctgg tcatttgcgc
    59581 catcggtcac attaccgctg ttcactggcg gaagtaatct ggcgcagctt cgttacgcag
    59641 aagcacaaaa gcgtgggctc attgccacct atgaaaaaac cgttcagagc gcatttaaag
    59701 atgtcgctaa cgcactggcg cggcggacca cgcttgaaga gcaactggat gcacagagcc
    59761 agtatgttaa agctgagcaa caaacggtcg atgttggctt gcgtcgctat caggcgggtg
    59821 ttggtgatta cctgaccgtg ttgacggcgc agcgcagctt gtggagcgca cagcaggagc
    59881 tgctggcact gcaactgact gattttacaa accgaatcac gctctggcag tcgctgggcg
    59941 gcggtatgtc atcactcaag taaggaataa tatggctaga gtctctcttt catgggcatt
    60001 gattcttggt cttttagccg gtatcggccc gatgtgtacc gatctttatt tgccggcttt
    60061 gccggagatg tctgagcaac tggcggcaac cacgaccata acgcaattaa ccctgactgc
    60121 atcactgatt ggtcttggcg tcggacaact gttatttggc cctctgagcg acaaaatagg
    60181 gcgtaaacgt ccgttaatct tgtcgttgct attgtttatt gtttcttcca ttttatgcgc
    60241 gacaacgaac aatatttact ggctggtggt ctggcgtttt attcaaggga tcgcgggggc
    60301 gggtggttcg gtgctctctc gttctattgc tcgtgacaaa tatcagggag taacgttgac
    60361 ccagtttttt gcgctgttaa tgacggtgaa tggcctggca ccggtgttgt cgccagtgct
    60421 gggcgggtac attgttagca cttttgactg gcgcacttta ttctgggtaa tggctgaaat
    60481 tagcaccgta ctgttgctgg gctgcctgtt atttattaat gagaccttgc cagaaaataa
    60541 aaggggctca tcattgctat taaccggacg aagcgtggtg cagaaccgcc gttttatgcg
    60601 cttttgcctg attcaaagtt ttatgctggc tggtttgttt gcatatatcg gctcttcgtc
    60661 gttcgtgttg cagaaggaat ttggctttag tccaatgcaa tttagcctgg tgtttggcct
    60721 taacggcatc ggacttatca ttgcttcatg gatcttctcg cgcctggcgc gacggattaa
    60781 cacgatgaca ttgttgcgag gtggcctgat agcggcaatt ttgtgtgcat tgctcacggt
    60841 cttatgcgca tgggtacaat tgcccattcc ggcactggtg gcattatttt tcaccatcgc
    60901 attttgtagc ggcatcggca ctgttggcgg ggcagaggct atgagtgcag tagggacgca
    60961 ggaatctgga acggcgtctg cgttgatggg gatgagcatg tttgtcttcg gcggtatagc
    61021 cgcgccattg tcgggaattg gcggagaaac actgttaaaa atgagtctgg caattacggt
    61081 gtgttatacg ctggcattgc tggttgctct caccagaatc gacaatcaaa agtaatgaac
    61141 acctcatcct gaggggggcc tgtcaggatg aggtgcaaaa cactaacggt ccttacgcca
    61201 cgcatccgcc gtcaatgctt cgccaaaatg accggcgatc agccgtttgg tgagttcatg
    61261 aagcggcgat gccagcacat ctgcggtgct gcctcgctcg acaacctcgc cctgatgcat
    61321 caccagcacc tggtcgctaa tgtgcttcat cattccgata tgctgggtaa cataaatata
    61381 cgaaatgccc tgtttttcct gtaactccag catcagatta atcaactgcg aacgcatcga
    61441 catatccagt gaggcgaggg cttcatcggc aataatgact tttgggcgca atatcagcgc
    61501 gcgtgccaga cccagacgct gtttttgccc gggtgctaac atatgcggat agtaactgac
    61561 gtgatccggt agcagcccaa ccatacgcat cgtttcaata atctgtttgc gacgctgttc
    61621 cggttccagg tcggtgttca ggcgcagtgg aaaatccaga atttgcgaga tacgttgacg
    61681 gggattcaac gaggtcgaag gatcctgaaa aatcatgcga atacgctgac tgcggaagga
    61741 ataatcgcca aaatgcagcg gatgatcgtc aatcaataac tcgccgctgg tcggttctat
    61801 cattcccgcc agcatttttg ccagcgtgga tttacccgaa ccattctcgc cgataatcgc
    61861 cagtgtctgg ccttcacgta gcgtaaagct caagggtttt accgcttcta cggtctgacg
    61921 acgaaaccag ccggtccggt agcggaacgt tttacttaaa ttgcgcactt caagtagcgt
    61981 ttcgatcatc tcactctttc tccatgttca gcgggaaatg acaggcatag agatgatttt
    62041 tcgcccccgt caaacgtggc gtcacaatgc attctcgttg tgcatacggg caacgtggcc
    62101 ccagacgaca accaatcggt aactgttcca gcagcgggat agcgccgggc agcgtattga
    62161 ggcgactttt atgcggcatc gcgctgccga agtctggtat cgcgcggatc agcgcctggg
    62221 tataaggatg gtgtggcatc gtcaccaact ccttactcgg cgcggtttcc accgtttgac
    62281 cgcagtaaag cacgttaatt ttatccgccc attggctaag catttgtaag tcatggctga
    62341 taagcaaaat agtggtattg ctgttttggt tgagacgcgt cagcaggcga aagatttgcg
    62401 cctgggttgt tggctccatt gagttggtcg gttcgtcagc aattagcaga cgcggttgat
    62461 tcgccagtgc aatggctatc atcactttct gacattcacc ttcggtcaac tcatagggaa
    62521 aactgcgcat cgcatctttg tgatctttaa tccccacgcg gtgcagcagt tcaatggcac
    62581 ggcgtttgcg ccagccaaaa cgctgccacc aacggccttt ataggtccag gcagggatgt
    62641 tttgcatcaa ctggcgaccc acacgttctg aagggtcaag acacgactgc ggttcctgga
    62701 aaatcatcga cacgttgtga ccaaccagct tgcgccgttc gcgtgcggag agacgcagca
    62761 aatcgatatc atcaaaacgc atacggtcag cagtaacacg ccagttatct ttattcaccc
    62821 cacaaatcgc tttcgcaatc aaacttttgc cggaaccgga ttcgccaaca agaccgcgga
    62881 tttcaccttc ggttaaggtc atacttacgc ggtcgacggc tttaacccac tcatcaccgg
    62941 ttttaaattc aatggtcagg ttacgaatat cgagtaatgg cattattcca cccccgcaat
    63001 aattgcacga cggacaccgt cgccaaggag gttaaccaac agcacgctaa tcataattgc
    63061 cgcacctggc agcatgacag tccatggggc gacataaatc agttccagcg catcaccgag
    63121 catcgcgccc cattcaggcg aggggagttg tgcgccgaga tcgagaaagc ccagcgcggc
    63181 gatatcgaga attgccatcg acagtgcgcg ggtgatctcg gtgaccagcc cggcggtgat
    63241 gttgggcatc acagcaaacc agagaatatt cagcgttgat gcgccatcca gacgggcggc
    63301 gataacgtac tctttttcca gttcgtcatg caccatgctg taaatcgaac gtaccatacg
    63361 cggcagcagc gccagccaga cagcaaacat ggcgtgagac aaactcggtc cggcaaacgc
    63421 tacaacgata attgccagca gcagcgaagg gatcgccagt aaggtatcca gaatatggtt
    63481 aagcaccgct gagcgtaggc cgtgggtcgc cccggcgaag gtgcccagta ccaggccaca
    63541 gatcgtcgcg gcgagcgtca ccacaaatgc gccacctacg gttggcgcag ctccgctcag
    63601 taaacggctt aaaacatcgc gtccgaggtc gtcagtcccc aggaagaaag aaacttcgcc
    63661 atagcgcgac catgacggcg gcaataattg ataaccgaga aattgctggt cgatgccgta
    63721 aggcgcaaac cagccgccaa aaatacacag tacagccagc cccgcacagc cgtacaggcc
    63781 gaccatcgca gaggcgtcac tataaaattt gcgccaggcg gtacgcagcg tgcccggcgg
    63841 gcgcttttcg ctgtatacgc tatcgtaagg cataccattc cttatgtttc agagggttag
    63901 ccatggcacc caaaatatca gaaatcacgt taacaataat gaccagtgag ccacacacca
    63961 tcactccggc ggaaatggct gcgtaatcct gctggcggat ggcgttaatt aaccagcgcc
    64021 ctaaacccgg ccagctaaaa accatttcgg tgatcatcgc cagcgtcagc atggtggaaa
    64081 actgtaagcc caggcgagga ataaccggag gtaacgcgtt atgcagaacg tggcgacgca
    64141 aaatagtaaa gcgcgacaaa ccgcgggttg ccgctgcttt aacatagttc tggtcataca
    64201 cttcgatagt gctgatacgc atcagtcgga tcacttcagt tgttggcgca accgacaggg
    64261 tgagcacggg caataccata tgacggattg cgctaatgat catttcatcc cgccacggtg
    64321 agtccgaaag ccaggcatca atcaacgcaa aaccggtaat cggtttcact tcgtagagca
    64381 gatcgaaacg ccctgaaacg ggcaaccaac ctagcgtgag tgaacaaaac agcgtcaaca
    64441 gaagcgccag ccagaacacc gggattgaaa agcccagtaa ggcgatggcg ttgatcaagt
    64501 tatcctgcca cttatggcgc gtaatcccgg caatcatccc cacggggatg ccaacaatca
    64561 gcgcaaaacc gaaggcgagg atgcacagtt ccatcgtcgc cgggaagacc tcttttaact
    64621 gttcggcaat cggttgaccg ttaatgctgg aaacaccaaa atcccagtgg atcaggccgt
    64681 taaaccagaa cacccaggca ttccacagtg acgcgccttg taacggcgca tgtggggtga
    64741 aatagctcaa gctgaagcca acgaaggtca gcaggaacaa ggtgacaatc aataacaaaa
    64801 tacggcgtaa ggtgaagata atcatggctt tttcacctca tcctgtttct cgcgatacac
    64861 cccagcaaag gaggcgttac caaacgggct aagtaccaga cctttgatat cgtaccgata
    64921 ggcctgcaaa cgcaatgacg acgccagcgg cagaataggt aattcctgcg ccagaatact
    64981 ttgcgcttcg tcataggctt caatacgcgc cgccagctgc tgcgaagaga gcgccttacg
    65041 caatacgctg tcgaatttcg gatcgcacca gtgggcgagg ttggtctgcg aatgaattgc
    65101 tgcgcagctc agtaacggac ggaagaaact gtccgggtcg ttactgtccg tcgcccaacc
    65161 ggataacgtc agatcatggc tcatatccat caaccgcgcc tcctgaaaac gaccttctac
    65221 cggcacaatc accactttta cgccaacctg tgccatatcc gcctgaatca gttcggcggt
    65281 tttcagtgga ctggggttcc acgcctgcga acgtgtgggc acccacagtt tgagcgttaa
    65341 gttttccagc cccaacgcct tcaactgttc gcgcgatttt gccggattgt attcagtaat
    65401 tttagcctcg ttgtcatagg cccacgaggc tcgcggtaaa atagaggcag ccgtttcagc
    65461 cgtaccataa tagatggatt gcatcaggcg ctggttatta atcgccagtg ccagcgcatg
    65521 gcggacagcg ggattattta gcggcggttt agcggtgtta aacgccagat aggcgacgtt
    65581 catccccggg cgcagcgtta aacgcaagcg tgggtcgtca cgcaaaatgg atagctgact
    65641 ggcagcaggc caggccagaa cgtcgcactc cccggtcagg agtttcgaca atcgtcctgt
    65701 accgccagag cctaaatcca ctaccacttg tggcatcaac ggcttaccac gccagaaatc
    65761 atcatgacgt tgtaagcgaa tatattgccc ggcgcggtat tccgacaact gatacggacc
    65821 ggtgccgacc ggttgacggt cgagttgctc ctggcgatct tctttctcta acttccctgc
    65881 atattctgcc gacatgaccg aagcataatg ggtcgccagg tgccacaaaa aagaagcatc
    65941 cggctgggcg agacggaact caacggtatg attatccagc ttgcggacgc ttttcacgtt
    66001 atcggcaaat tgcagactgt cgaaataggg gaagttactg ccgttgacgt tatgccacgg
    66061 gttgttgcgg tcaaaaatcc gttggaaggt aaacaccaca tcatcggcat tcattttacg
    66121 agtgggagta aaccagtcgg ttttttgaaa cggaacatcg cgacgcaggt ggaagcgata
    66181 ggtcgcgccg ttgtcgagca cttcccagct ttcggcaagt tccggcatca ggcgataggt
    66241 atagggatcg acatccagca gtcgatcata aaactgggcg gcaagggtat cgacaattaa
    66301 cccactgctc gctttggatg ggttaaaggt gttgacttgc ccgctgacgc aatagacaaa
    66361 accgctgtcg cggatatcag catgcggggg ggattcaggc gcggcgattg cctgaccact
    66421 cacaagtcca gcaatcacca aaagagacga taatacctgg cgcataatat taagggattt
    66481 tatgtaaaga ggctatctta ctaatattta atgacatttg ccattaccgt ttgtgttcag
    66541 aggtcgtaat gagggccgtg tggttggtca ttgaacaggt ggcgtcgcca tcagttcaac
    66601 attcgtaccc gtgatttctt tataactata actcacagaa caacttagcg aggagcatag
    66661 cggtacgatc gtgagaaacc cattaaagca tgctgcagtg cgtgtcattt ttagccggat
    66721 gcggcgcgaa cgctttatcc gggcaacgat actgaccgat agtctgcaat taaataatta
    66781 ctcattaccc cattgattca aaaactctgc gatctcatca atgcgtacgg gattaatccc
    66841 cgcttcagca gccatttcat gttgggcttc ttcactgatc tcttcattat tcatcaaacg
    66901 ggtgagcagt aactggaagt agtgggccag cggtgtgcgt tcagcgtctg cgacaggctc
    66961 tgcgcattcg gtcgcgtact catcggcaat atcaaaatat tttagcggga tatcgtggtt
    67021 cattatttgc ccttggggtt aatgcgctaa gcataaaggc ggatgatagc actcgtgatc
    67081 cgggtcatac atatttttat agttaatggc gcggtgcatt accctcaggc aggataatgc
    67141 gccgcgcatt cgactattac gtttcgttca ccggcgttct gtcgccatca tacaacggca
    67201 ctttgcgata gcggcggatc aaataccaca aatatgcgcc gcctaacgaa gcccatacca
    67261 tcccggttgc gcaattcgat gcggcaatgc aaattttgca ggatgcgctg agagattaat
    67321 acgcagaagc agggttaacg caataatgcc tgatgcgcag cgcttatcag gcctgcaaac
    67381 gacgtattga ttatgtatgc cggataaagc gttcacgctg catccggcaa gttgtattgc
    67441 ccaacttcgc taaatctggt gctttttcaa caacgcgcgg aactgatgat aggttaaccc
    67501 cagcaattca gcggcgcgct tctggttata tttcccctgt tgcaaactga gttgcagcaa
    67561 ctctttttcc tgctgcatct gaaactcgcg taaatccagt ggcagcgtcg gaagcgaggt
    67621 gttttctgaa acggcgatgg cttcttcaga tggacggcgt ttaaagggat caataatgat
    67681 gtcatcaagc ggataatcgc tggtgccgtg acgatacact gaacgttcca ccacgttttt
    67741 caattcacgt atatttcccg gccagcgata attcagcaat gtttctctgg cgtgctcggt
    67801 aaaacccggg aacagaggca gcttgatttc ccgacacatc tggatggcaa agtgttctgc
    67861 catcaacata atgtcgcttt tacgttcgcg tagtggcggc agttgcacaa catcgaacgc
    67921 tagtcggtcg agcaggtcag cgcgaaaagt accttcattg accatcgccg ggagatcggc
    67981 attcgtcgcg cataccaacc gcacattcac ctgcaatggc tggctgccgc cgacgcgctc
    68041 cagttcaccg tactcaatca cacgcaataa tttttcctgc accatcatcg gtgccgtagc
    68101 gagttcatca agaaatagcg taccgccgtc ggcacgttca aatctccctg gatgacgttt
    68161 ttgcgcaccg gtaaacgccc ccgcttcatg accaaacagt tcggaatcca gcaaattttc
    68221 atttaacgcc gcgcagttaa gggaaataaa cggcccttgc caacgggagg agagataatg
    68281 caggcggctg gcgatcagct ctttaccggt gccgcgctcg ccgatgatga gcaccggttt
    68341 gtccagcggt gcgagatgcg aaacctgttc cagcacttcg agaaagctgt tcgcctcacc
    68401 gagtaaatta tctttgtatt ctgccatgat gaaattcgcc acttgttagt gtaattcgct
    68461 aattcatcct ggcatgttgc tgttgattct tcaatcagat ctttataaat caaaaagata
    68521 aaaaattggc acgcaaattg tattaacagt tcagcaggac aatcctgaac gcagaaatca
    68581 agaggacaac attatgggta ttttttctcg ctttgccgac atcgtgaatg ccaacatcaa
    68641 cgctctgtta gagaaagcgg aagatccaca gaaactggtg cgtctgatga tccaggagat
    68701 ggaagataca ttggttgaag tacgttctac ttcggcgcgt gcgctggcag aaaaggaaca
    68761 gctgactcgt cgtattgaac aagcgtcggc gcgtgaagtt gaatggcagg aaaaagccga
    68821 actggcgctg ctgaaagaga gagaagatct ggcgcgtgcg gcgttaattg aaaaacagaa
    68881 actgaccgat ctgattaaga gcctggaaca tgaagtgacg ctggtggacg atacgctggc
    68941 acgcatgaag aaagagatcg gcgagctgga aaacaaattg agcgaaacac gcgctcgcca
    69001 gcaggcattg atgttacgcc atcaggcggc aaactcgtcg cgcgatgtgc gtcgtcagct
    69061 ggacagtggc aaactggatg aagcaatggc tcgtttcgaa tctttcgaac gccgtatcga
    69121 ccagatggaa gcggaagcag aaagccacag cttcggtaaa caaaaatcgc tggacgatca
    69181 gtttgccgaa ctgaaagccg atgatgcaat cagcgagcaa ctggcacaat taaaagccaa
    69241 aatgaagcaa gacaatcaat aataacatcc aggcggcgtc cgaacgcgcc gccgctcatc
    69301 gtctaaggag tacttatgag cgcgctattt ctggctattc cgttaaccat ttttgtgctg
    69361 tttgttttac cgatctggtt atggctgcat tacagcaatc gttacggtcg cagtgaattg
    69421 tcgcaaagtg agcagcagcg attagcgcaa ctggttgatg aagcaaaacg gatgcgcgag
    69481 cgtattcagg cgctggaatc cattcttgat gcagaacatc cgaactggag ggatcgctaa
    69541 tggcgggcat taatctcaat aaaaaattat ggcgtattcc gcagcagggc atggtccgtg
    69601 gcgtctgcgc cgggattgcc aactattttg atgtaccagt aaaactggtg cgtatcctgg
    69661 tggtgctgtc gattttcttc ggtctggcgc tgtttaccct ggttgcttac atcattttgt
    69721 catttgcgct tgatccaatg ccggacaaca tggcctttgg tgagcagcta cctttcagca
    69781 gcgaattgct ggatgaagtc gaccgtgaac tggcggcaag tgaaacgcgt ttacgcgaga
    69841 tggaacgtta tgtcacttcc gacactttca cgctgcgtag ccgtttccgt caactgtgag
    69901 gaaagttatg aatactcgct ggcaacaggc cgggcaaaag gtgaagcctg ggttcaaatt
    69961 agcaggcaag ctggtacttc ttaccgcact gcgctatggc ccggcgggtg tggcgggctg
    70021 ggcgataaaa tcagttgctc gccgaccgct gaaaatgttg ctggctgtgg cgctggaacc
    70081 gctgttaagc cgggctgcca ataaactggc gcagcgttat aaaaggtgag gggagtttcg
    70141 caaaaatggt taaatctcag gcgtataatg gatagcaatt ttcatccata gaaggacgct
    70201 tacatgttta aaaaaggctt acttgctctg acactggtgt tttcactgcc cgttttcgcc
    70261 gctgaacact ggatcgatgt tcgtgttcca gagcagtatc agcaagaaca cgttcagggg
    70321 gccatcaata ttcccctgaa ggaagtgaaa gagcgcattg ccaccgccgt tccggataaa
    70381 aacgacaccg tgaaagtgta ttgcaatgcc ggacgccagt cagggcaggc aaaagagagc
    70441 cttagcgaga tgggatatac ccacgttgag aacgccggtg gcctgaaaga catcgcaatg
    70501 ccgaaggtca aaggttaaaa gaattcacca tgagcggcgc ttatgcgccg ttttttctgt
    70561 cacaccttat ttacatcccc atagattatt tgcgttagct cacaaatacg ctttttccca
    70621 ggtaaaaaaa gatttcctgc gtgactaaac cccttgagct caattgacag tttattttat
    70681 gcggagtagt ctctcgcttc atgggaccgc taccacggaa aggcaacatg aaacagaaaa
    70741 ttacggatta cctggacgaa atctacggtg gaacatttac cgcgactcat ttacagaaac
    70801 ttgtaacgcg tcttgagagt gcgaaacgat taattacaca gcgacgtaaa aaacactggg
    70861 atgaaagtga tgtcgtgtta attacctatg ccgatcaatt tcacagtaat gatttaaagc
    70921 cactacccac atttaatcag ttttaccctc aatggctgca aagaattttt tcacatgttc
    70981 atttattacc tttttatcca tggtcatctg atgatggatt ttcggtaatt gattatcatc
    71041 aggtcgccag tgaagcgggg gagtggcagg atattcagca actcggtgaa tgcagccatt
    71101 taatgtttga ttttgtctgc aaccatatgt cggcaaaaag tgaatggttt aaaaactatt
    71161 tacaacagca gccaggtttt gaagattttt ttattgccgt tgatccgaaa accgatctca
    71221 gcgccgtcat tcgcccgcgt gcgttaccgt tattaacgcc attccagatg cgcgatcatt
    71281 caatgcgcca tttatggacc acctttagcg acgatcaaat tgacctcaat taccgtagcc
    71341 ctgaagtgtt gctggcgatg gtggatgtat tgctgtgcta cctggaaaag ggcgcagagt
    71401 atgtccgtct ggatgccgtt ggctttatgt ggaaagagcc gggaacaagc tgcatccatc
    71461 tggaaaaaac acatctgatt atcaaactgt tacggtcgat tattgataac gtcgcgccag
    71521 gcacagtgat cattaccgag accaatgttc cgcataaaga caacattgct tactttggcg
    71581 aaggcgatga cgaagcgcat atggtgtacc agttctcgct gccgccgctg gtgctgcatg
    71641 cggtgcaaaa acagaacgtt gaggcacttt gtgcgtgggc gcaaaacctg acactacctt
    71701 ccagcaacac cacctggttt aacttccttg cctctcacga tggcatcggg cttaacccac
    71761 tgcgggggtt gttgcccgaa aacgaaatat tagcgctggt cgaggtgtta cagcaggaag
    71821 gggcattagt aaactggaaa aataatcccg acggtacgcg tagcccctat gaaatgaatg
    71881 tcacctatat ggatgcgttg agccgccgtg agtgtagcga cgaagaacgg tgtgccaggt
    71941 ttattctcgc ccatgcgatt ttgttaagtt ttcccggtgt gccagcgata tatattcaaa
    72001 gcattctggg gtcgcgtaat gattacgcag gtgtcgaaaa acttggatat aatcgcgcga
    72061 ttaaccgtaa aaaatactct agtaaagaga taacgacaga actgaacaat aaagcgacat
    72121 taaggtattc gatatattat aagttgtcgc atttaattac acttcgtcgc agtcataaag
    72181 agtttcatcc ggataataat ttcaccattg atgcgattaa ttcatttgta atgcgaattc
    72241 aaagaagcaa tgcggatggc gattgcctga cgg
//
LOCUS       DAZFJL010000004        69734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFJL010000004 DAZFJL010000000
VERSION     DAZFJL010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.004"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            12..680
                     /gene="yjcO"
                     /locus_tag="ACHJ8C_000262"
     CDS             12..680
                     /gene="yjcO"
                     /locus_tag="ACHJ8C_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO5550182.1"
                     /translation="MLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADSWFSSGDL
                     SKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSKEGEVTLA
                     HILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKADDDKATW
                     YFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDTGCEEFEK
                     LTNG"
     gene            complement(1620..2933)
                     /gene="gltP"
                     /locus_tag="ACHJ8C_000263"
     CDS             complement(1620..2933)
                     /gene="gltP"
                     /locus_tag="ACHJ8C_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO5550183.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(3276..3872)
                     /gene="nrfG"
                     /locus_tag="ACHJ8C_000264"
     CDS             complement(3276..3872)
                     /gene="nrfG"
                     /locus_tag="ACHJ8C_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO5550184.1"
                     /translation="MKQPQIPVKMLTTLTIFMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTVQARAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(3869..4252)
                     /gene="nrfF"
                     /locus_tag="ACHJ8C_000265"
     CDS             complement(3869..4252)
                     /gene="nrfF"
                     /locus_tag="ACHJ8C_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO5550185.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQEQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYTMVEEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMTLILWRVRAKR"
     gene            complement(4245..5963)
                     /gene="nrfE"
                     /locus_tag="ACHJ8C_000266"
     CDS             complement(4245..5963)
                     /gene="nrfE"
                     /locus_tag="ACHJ8C_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO5550186.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGTCARICWRWALLGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFALLMLVVIVLATFVSGKRAQLPALLAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            complement(5983..6939)
                     /gene="nrfD"
                     /locus_tag="ACHJ8C_000267"
     CDS             complement(5983..6939)
                     /gene="nrfD"
                     /locus_tag="ACHJ8C_000267"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO5550187.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRKNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(6936..7607)
                     /gene="nrfC"
                     /locus_tag="ACHJ8C_000268"
     CDS             complement(6936..7607)
                     /gene="nrfC"
                     /locus_tag="ACHJ8C_000268"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO5550188.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTHL
                     QAGKLPACVEACPTKALTFGNLDDPSSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(7604..8170)
                     /gene="nrfB"
                     /locus_tag="ACHJ8C_000269"
     CDS             complement(7604..8170)
                     /gene="nrfB"
                     /locus_tag="ACHJ8C_000269"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO5550189.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(8215..9651)
                     /gene="nrfA"
                     /locus_tag="ACHJ8C_000270"
     CDS             complement(8215..9651)
                     /gene="nrfA"
                     /locus_tag="ACHJ8C_000270"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO5550190.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPATPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            10043..12001
                     /gene="acs"
                     /locus_tag="ACHJ8C_000271"
     CDS             10043..12001
                     /gene="acs"
                     /locus_tag="ACHJ8C_000271"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO5550191.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWITPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSSSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASAQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            12213..12527
                     /gene="yjcH"
                     /locus_tag="ACHJ8C_000272"
     CDS             12213..12527
                     /gene="yjcH"
                     /locus_tag="ACHJ8C_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO5550192.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            12524..14173
                     /gene="actP"
                     /locus_tag="ACHJ8C_000273"
     CDS             12524..14173
                     /gene="actP"
                     /locus_tag="ACHJ8C_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO5550193.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(14212..14901)
                     /locus_tag="ACHJ8C_000274"
     CDS             complement(14212..14901)
                     /locus_tag="ACHJ8C_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFO5550194.1"
                     /translation="MANFQLSMLCLAITLVIYFLNKRLYRRFRKLPLMPLVLTPTLLV
                     MLLVFGNISWQNYIGEAHWLLWLLGPATIAFAVPVYDNRAIIKRHWMSLTAGVLTATV
                     VAVTSSVWLARLFTLPDEIQRSLAVRSVTTPFALAASQSLGGQPDLVALFVVVTGVFG
                     MAIGDVLFLRLSIREGMAKGAGFGAASHGAGTARSYELGQQEGVVASLVMMLSGVLMV
                     LVSPLVARMMF"
     gene            complement(14894..15304)
                     /locus_tag="ACHJ8C_000275"
     CDS             complement(14894..15304)
                     /locus_tag="ACHJ8C_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO5550195.1"
                     /translation="MPVALRRITPAVVQSIQVFFQVVLYAGLFIFAQYLVSWLHLPLP
                     ANLVGMILMLALIVCRILPLQWVRAGARWLLAEMLLFFVPAVVAVVNYAQLLLVDGWR
                     IFAVIALSTVMVLGTTAWVVEKVYRYEMRRLNRG"
     gene            15408..16292
                     /locus_tag="ACHJ8C_000276"
     CDS             15408..16292
                     /locus_tag="ACHJ8C_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5550196.1"
                     /translation="MDIRTLRYFVEVVRQQSFTRAAEKLFVTQPTISKMLKNLEDELN
                     CILLIRDGRRLLLTDTGRVVFERGLALLAEFRQLEAELSDINQLKTGVLRLGIPPMVG
                     MLMAGPIGLFRQRYPGVELKISEFGGLTVQQAVMNGELDMALTALPVEESGLTTLSLF
                     SHPLCVLTPHSGKWLECDAVSPETLAEHPLLIYNEDFALSKQLMKLFDEHGVKPRIAV
                     RSGQWDFLAAMVQAGVGIAILPEPICQRLDKNTLKWLPLESELSWKLGMIWREGVYLS
                     HSAHAWLSCCEGYWLTPE"
     gene            complement(16328..17977)
                     /gene="yjcE"
                     /locus_tag="ACHJ8C_000277"
     CDS             complement(16328..17977)
                     /gene="yjcE"
                     /locus_tag="ACHJ8C_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO5550197.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSILEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(18128..19477)
                     /gene="ghxP"
                     /locus_tag="ACHJ8C_000278"
                     /gene_synonym="yjcD"
     CDS             complement(18128..19477)
                     /gene="ghxP"
                     /locus_tag="ACHJ8C_000278"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO5550198.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFTTFPVMMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(20025..20489)
                     /gene="soxR"
                     /locus_tag="ACHJ8C_000279"
     CDS             complement(20025..20489)
                     /gene="soxR"
                     /locus_tag="ACHJ8C_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO5550199.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     ERRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            20575..20898
                     /gene="soxS"
                     /locus_tag="ACHJ8C_000280"
     CDS             20575..20898
                     /gene="soxS"
                     /locus_tag="ACHJ8C_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO5550200.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(20901..22487)
                     /gene="pdeC"
                     /locus_tag="ACHJ8C_000281"
     CDS             complement(20901..22487)
                     /gene="pdeC"
                     /locus_tag="ACHJ8C_000281"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO5550201.1"
                     /translation="MSHRARHQLLAFPGIIFLVLFPIILSLWIAFFWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIASVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHTDRYVSINLSASDFHTSRLIARTNQKTEQHAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRKAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEAQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            22917..23198
                     /gene="yjcB"
                     /locus_tag="ACHJ8C_000282"
     CDS             22917..23198
                     /gene="yjcB"
                     /locus_tag="ACHJ8C_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO5550202.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRADYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(23297..23833)
                     /gene="ssb1"
                     /locus_tag="ACHJ8C_000283"
     CDS             complement(23297..23833)
                     /gene="ssb1"
                     /locus_tag="ACHJ8C_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO5550203.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            24088..26910
                     /gene="uvrA"
                     /locus_tag="ACHJ8C_000284"
     CDS             24088..26910
                     /gene="uvrA"
                     /locus_tag="ACHJ8C_000284"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO5550204.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYINDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(26945..27301)
                     /gene="yjbR"
                     /locus_tag="ACHJ8C_000285"
     CDS             complement(26945..27301)
                     /gene="yjbR"
                     /locus_tag="ACHJ8C_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO5550205.1"
                     /translation="MTISELLQYCMAKPGAGQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(27305..27721)
                     /gene="yjbQ"
                     /locus_tag="ACHJ8C_000286"
     CDS             complement(27305..27721)
                     /gene="yjbQ"
                     /locus_tag="ACHJ8C_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO5550206.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRAVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(27832..28545)
                     /gene="aphA"
                     /locus_tag="ACHJ8C_000287"
     CDS             complement(27832..28545)
                     /gene="aphA"
                     /locus_tag="ACHJ8C_000287"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO5550207.1"
                     /translation="MRKITQALSAVCLLFALNSSAVALASSPSPLNPGTNVAKLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            28822..30642
                     /locus_tag="ACHJ8C_000288"
     CDS             28822..30642
                     /locus_tag="ACHJ8C_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5550208.1"
                     /translation="MKISWNYIFKNKWRFHITSISLFLIMLAVSIAFLHLRFNTFSGT
                     DKMRLEMYKSTLYSTIEQFYVLPYMLSTDHIIRQAVITPDDMTSSELNQRIAHFNTQL
                     KTAAIFILDTQGKAIASSNWQDPGSYVGQNYSYRPYYKHAMSGLNGRFYGIGSTTNTP
                     GFFLSTSIKDKGKIVGVVVVKISLNEIEKAWAEGPENIIVNDEHGIIFLSSKSPWRMR
                     TLQPLPVQAKQKLQSTRQYSLDNLLPADYYPCYTVSNFTFLKDKKEQLCLFPQYYTQQ
                     IAIPEFNWKMTIMVPLDNLYWSWAILLVITLIIYLLFLLFIKYWRMRSHAQQLLTLAN
                     ETLEKQVKERTSALELINQKLIQEIKERSQAEQVLQITRSELAESSKLAALGQMATEI
                     AHEQNQPLAAIHALTDNARTMLKKEMYPQVEQNLKHIISVIERMTQLISELKAFASRH
                     RVPKGSADVIKVMYSAVALLNHSMEKNNIERRIKAPSMPLFVNCDELGLEQIFSNLIS
                     NALDSMEGSSYKRLDIAIRQANNKVIITIKDSGGGFAPEVVDRIFEPFFTTKRRGMGL
                     GLAIVSEIVRNSNGALHASNHPEGGAVMTLTWPEWGEEHE"
     gene            30635..31993
                     /locus_tag="ACHJ8C_000289"
     CDS             30635..31993
                     /locus_tag="ACHJ8C_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFO5550209.1"
                     /translation="MNNKSSEITIVYIEDSDDVRFACEQTLTLAGYRVISCCDAEHSI
                     PLIQSQANIIILTDVRLPGISGLELLSYINEMDSKIPVILITGHGDVEMAVDAMRNGA
                     FDFIEKPSSSDKLLSIIARAVEKRRLVLENQQLLANLQQENGPVLIGRSPQMQQLRKM
                     ILNVADTGADVLIYGETGCGKEVVARMLHHWSTRRQGQFVALNCAGLPETLFESEIFG
                     HEAGAFTGAVKKRIGKIEHANGGTLFLDEIEGMPSGMQVKLLRVLQERTIERLGANQL
                     IPVNCRVIAATKEDLLRRSEEHLFRLDLYYRLNVVSLNIPPLRQRREDIPELFYWFAS
                     QAAQKYNRPLPDISPMLLAWLQSQSWPGNVRELKHNAERFVLGLLTHHQPVPMTQQEE
                     SGLTACIDAFEKKLIEDMLRQTEGQVSLTARLLQLPRKTLYDKLNKHQIQPQVYRPES
                     SS"
     gene            complement(31982..33055)
                     /locus_tag="ACHJ8C_000290"
     CDS             complement(31982..33055)
                     /locus_tag="ACHJ8C_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HFO5550210.1"
                     /translation="MSLQIAVEKVRWLAAGLLELNGCDADIAQDVAEHMIEAERYGFA
                     SHGVTLLPKYLENIARGDVTANARPECLTSEGNLQRFHAHNGFGQHAGKVAVNAAIEQ
                     AKHRGFCLLTLCHAHHLGRMGHYGQMVADQGLAMLAMSNVTGRPALVAPWGGAEPRMT
                     TNPFCFAWPFSDGRPPILVDFATSSMALNKARVMSSTGKQAAPGQLIDALGNPSNDPG
                     VLFSNPPGALLPFGEHKGFGLALMIEMMAGILSGGDTIAEDHQTDGSAHNHLFALIID
                     PDQFTDLAGEKGQFFADYLLATQPQPGGNPVIYPGMPEAANRERNAKMITIPVSFWSW
                     VVEHYARKGIDLGLHPQESALAG"
     gene            complement(33067..34566)
                     /locus_tag="ACHJ8C_000291"
     CDS             complement(33067..34566)
                     /locus_tag="ACHJ8C_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFO5550211.1"
                     /translation="MSSISHGAPQKRRIIPNPGLWLAIIAGIIITLLPLGDTLPVAGQ
                     NMIAILVFAIIVWISEAMDYTASAIVISALIIFMVGFAPDMNHPDTILGTAKALKMTL
                     SGFSNSALALVAAAMFIAAAMTITELDKRIALFTMSKIGASSRSIIIGAIVVTIVLSL
                     VVPSATARTACVVPIMMGVIAAFKVDKHSRLAASMMIVIAQATSIWNVGIQTSAAQNL
                     LSIGFINKTFGAGHSVSWLDWLLAGAPWSLTMSAILYFLARKLLPPETEAVEGGSEAI
                     KKALAELGPTTGKEKRLIGISLLLLLFWSTGGKLHSIDTTSVTLAGLAIMLLPGIGVM
                     SWKEVEKRVQWGTLLMFGIGISLGSTLLDTQAASWMANYVVKGFGLDGLPSLAIFAIL
                     AAFLIIIHLGFASATALTAALLPILISLLSSLPPELGVNPVGMTILLAFSVSFGFILP
                     INAPQNMVCMGTDTFTPRQFTRVGLYLTVIGYLLLLLFAATWWKILGLM"
     gene            complement(34778..35650)
                     /gene="sucD"
                     /locus_tag="ACHJ8C_000292"
     CDS             complement(34778..35650)
                     /gene="sucD"
                     /locus_tag="ACHJ8C_000292"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO5550212.1"
                     /translation="MSVLINKHTRVLVQGITGKNGTFHTEQAIAYGTQIVGGVTPGKG
                     GQRHLDRPVFDSMKEAMRQTEADASVIYVPAPFVLDSVVEAIEAGVKLVVVITEGVPT
                     LDMVKVRRLLDCHPDVRLIGPNCPGVITPGECKIGIMPGEIHRPGRIGIVSRSGTLTY
                     EAVAQTTALGLGQSTCIGIGGDPVPGTNFIDALRLFENDPQTDAVIMIGEIGGNAEER
                     AAEFIRHEMNKPVVGYIAGVTAPKGKRMGHAGAIISGGSGSAEDKFRAFDKAGMAWTR
                     NPALLGETLWNVIK"
     gene            complement(35663..36832)
                     /gene="sucC"
                     /locus_tag="ACHJ8C_000293"
     CDS             complement(35663..36832)
                     /gene="sucC"
                     /locus_tag="ACHJ8C_000293"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO5550213.1"
                     /translation="MNLHEYQAKSLLAGMGMPCPKEIAIQQISQLADAWQHIACPSKG
                     AVLKAQVHAGGRGKAGGVKVLKQLPEAQAFVQQMLGSQLVTYQTGPEGQYVSSILLCE
                     NIYPVRQELYFGMVVDRESQRVTFIVSPEGGVEIEKVAHETPEKISSVSIDPLTGVQP
                     CHIREMFAVLQLEHGLFATFSRLVNQAWKAFNELDFALLEINPLVLRETGEFMCADAK
                     VSLDDNALYRHPELQVLRDETQEDPRESQAAKLDLNYVSLDGNIGCMVNGAGLAMATM
                     DIIKLYGEQPANFLDVGGGATQERVSEAFRLIVSDSKVKAILVNIFGGIVRCDMIARA
                     IIHALNEARITLPVVVRLSGNNAAEGQRLLAESGLTVEAVNSLDDAAKRIIALLN"
     gene            complement(36854..38272)
                     /gene="lpdA"
                     /locus_tag="ACHJ8C_000294"
     CDS             complement(36854..38272)
                     /gene="lpdA"
                     /locus_tag="ACHJ8C_000294"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO5550214.1"
                     /translation="MSTIFDVAVMGGGPGGYVAALRAAQNGLSVVCIDDGVNAQGEPS
                     PGGTCLNVGCIPSKSLLQSSELYAQVQHEASIHGVNVDGVSFNAAAMIQRKDAIVSRL
                     TMGIGLLFKKNKVKYLCGLATLEHARDEIWQLRVNDQQIRARNVVIATGSQPRQLPGV
                     TIDNQQILDNRGALALTAVPPRLGVIGAGVIGLELGSVWNRVGSDVTLLEMAPTFLPA
                     LEARLSNEVRKAMIASGMKMQLAVEIEAIEQRDDGVHVRWRQGEKREESRFDKLILAI
                     GRVPRLSGVDLVQLGLEADNRGGIAVDNLCRTGKAGLWAIGDVVRGPMLAHKAMAEGV
                     VVADQIAGLAVEPINFALIPSVIYTQPEVAWVGENEASLKAAGRVFNKGNSLFAGNGR
                     ALALGQEGGRCTLYSDKHTDRVLGGAIVGPQASELINEIALAMTFSASGEDIACAIHA
                     HPTLSEVIHEAAMALNNKALHG"
     gene            complement(38284..39438)
                     /gene="odhB"
                     /locus_tag="ACHJ8C_000295"
     CDS             complement(38284..39438)
                     /gene="odhB"
                     /locus_tag="ACHJ8C_000295"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO5550215.1"
                     /translation="MIEITVPVLPESVTEGTLTTWCKQEGEHVKRDDVIAELETDKVI
                     LEIPAPHDGVLSNIIVSEGSTVTSAQLLAHLKPQAVIEETVTPVTETLAMPSARLEAQ
                     RSGVELADVAGSGRNGRILKEDVQRVTPAPATQPERVAEIAPAKPLTPGARHERREPM
                     SRLRQRIAERLLASQQNNAILTTFNEVNMQSVMDLRTRWKDRFAEKHGVKLGFMSFFV
                     KAVTRALERFPVVNASVDGNEIIWRDYCDIGIAVSSNRGLVVPVLRNAQSLSLGEIER
                     QIAEYATQARNGKLPLEALQGGTFSITNGGTFGSMMSTPIINPPQSAILGMHAITPRP
                     VAENGQVVIRPMMYLALSYDHRIIDGQEAVQTLVAIRELLESPEQLLLDL"
     gene            complement(39471..42215)
                     /locus_tag="ACHJ8C_000296"
     CDS             complement(39471..42215)
                     /locus_tag="ACHJ8C_000296"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO5550216.1"
                     /translation="MENMTSPGTLLSGDNATWLEEYYQTWLRTPEQLPEDWRRFFLSP
                     ELTVQSVSGDNNVSGATLKKQAAVIQLINAWRTQGHLRAKLDPLGLNPPADVPSLQPG
                     FWGLSEEDLLQEFSVTFGAHTTQMPLKQLLNLLEQAWAGSQAYELAHLENREEINWLL
                     SRIESSNAPQADAQTCIARFEKLMAAETLERYLHTRYVGQKRFSLEGGESAIPALDTL
                     TKRLRAQGVEEMVIGMAHRGRLNVLVNLLNKDPAQLFAEFEGKQTIGSGSGDVKYHMG
                     YSSNLETPAGSLHVALAYNPSHLEIVNPVVLGQVRARQERRGEDGQAKVVGVLIHGDS
                     ALGGLGVNQTTFNLSQTQGYGTGGTLHLVINNQIGFTTSRLQDMRSSRYCTDIAKMVA
                     APIIHVNGDDVDAVCQVMELACEWRDTFRRDIIIDICCFRKHGHNESDEPRLTQPQMY
                     QAVDAHPGTLARYGESLARRGLLTQAQQDEMTARYRDWLDSCQKREPQPLKPAIHSFS
                     ANWYGLTNPHWSAPVSTALPRQKLVAYGEIISTLPPDVVAHPTIKRQLALRQDMAAGT
                     QPVDWGMAEMLAYASLVDAGVGVRLSGEDSGRGTFSHRHAVVHHQTEARRYLPLQHIR
                     AGQASFDVYDSVLNEEALLAFEYGYSTSAPQQLVIWEAQFGDFANGAQVAIDQFISSG
                     ETKWDRYSGLTILLPHGYDGQGPEHSSARPERWLQLCAENNMQVVMPSESAQMFHLLR
                     GQALRPMRKPLVIMMSKRLLRFKGAMSELCEFTDGAYKPVITDPQLHQSQKVKRVILC
                     SGQVYYDVLEARKQREHEDEVAIVRLEQLYPFPVAELNDVLASWPNCCEWIWLQEEPE
                     NQGAWRQIRHELAALKINTPYWQYAGRPAAAAPATGYGRVHKQQIDEFLAAAFADIQP
                     "
     gene            complement(42397..43590)
                     /gene="tyrB"
                     /locus_tag="ACHJ8C_000297"
     CDS             complement(42397..43590)
                     /gene="tyrB"
                     /locus_tag="ACHJ8C_000297"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO5550217.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDSVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLRTAQVDRLREEFGVYLIASGRMCVAGLNAQNVHRVAKA
                     FAAVM"
     gene            complement(43843..44922)
                     /gene="alr"
                     /locus_tag="ACHJ8C_000298"
     CDS             complement(43843..44922)
                     /gene="alr"
                     /locus_tag="ACHJ8C_000298"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO5550218.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(44975..46390)
                     /gene="dnaB"
                     /locus_tag="ACHJ8C_000299"
     CDS             complement(44975..46390)
                     /gene="dnaB"
                     /locus_tag="ACHJ8C_000299"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO5550219.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            46473..47456
                     /gene="qorA"
                     /locus_tag="ACHJ8C_000300"
     CDS             46473..47456
                     /gene="qorA"
                     /locus_tag="ACHJ8C_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO5550220.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDARRAHEILESRATQGSSLLIP"
     gene            complement(47621..47863)
                     /gene="pspG"
                     /locus_tag="ACHJ8C_000301"
     CDS             complement(47621..47863)
                     /gene="pspG"
                     /locus_tag="ACHJ8C_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO5550221.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALIVATAFMFLGGMLALM
                     IKLLPWLLLAVAVVWVIRAIKAPKVPKYQRYDRWRY"
     gene            complement(47997..48992)
                     /gene="dusA"
                     /locus_tag="ACHJ8C_000302"
     CDS             complement(47997..48992)
                     /gene="dusA"
                     /locus_tag="ACHJ8C_000302"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO5550222.1"
                     /translation="MPAESHTVYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEM
                     VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPS
                     DRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
                     SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSL
                     GEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIEREL
                     SQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR"
     gene            49148..49663
                     /gene="zur"
                     /locus_tag="ACHJ8C_000303"
     CDS             49148..49663
                     /gene="zur"
                     /locus_tag="ACHJ8C_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO5550223.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALEFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCSACVEVEACRHPEQCHH
                     DHSIQVKKKPR"
     gene            complement(49705..49914)
                     /gene="yjbJ"
                     /locus_tag="ACHJ8C_000304"
     CDS             complement(49705..49914)
                     /gene="yjbJ"
                     /locus_tag="ACHJ8C_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO5550224.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVDSWEKRHDYRW"
     gene            complement(50030..51355)
                     /gene="dinF"
                     /locus_tag="ACHJ8C_000305"
     CDS             complement(50030..51355)
                     /gene="dinF"
                     /locus_tag="ACHJ8C_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO5550225.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRHLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNDTWFVAT"
     gene            complement(51428..52036)
                     /gene="lexA"
                     /locus_tag="ACHJ8C_000306"
     CDS             complement(51428..52036)
                     /gene="lexA"
                     /locus_tag="ACHJ8C_000306"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO5550226.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(52146..52514)
                     /gene="dgkA"
                     /locus_tag="ACHJ8C_000307"
     CDS             complement(52146..52514)
                     /gene="dgkA"
                     /locus_tag="ACHJ8C_000307"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO5550227.1"
                     /translation="MANNTTGFIRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            52685..55108
                     /gene="plsB"
                     /locus_tag="ACHJ8C_000308"
     CDS             52685..55108
                     /gene="plsB"
                     /locus_tag="ACHJ8C_000308"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO5550228.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(55263..56135)
                     /gene="ubiA"
                     /locus_tag="ACHJ8C_000309"
     CDS             complement(55263..56135)
                     /gene="ubiA"
                     /locus_tag="ACHJ8C_000309"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO5550229.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(56148..56645)
                     /gene="ubiC"
                     /locus_tag="ACHJ8C_000310"
     CDS             complement(56148..56645)
                     /gene="ubiC"
                     /locus_tag="ACHJ8C_000310"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO5550230.1"
                     /translation="MSHPALTQLRALRYFTEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(56824..57744)
                     /gene="malM"
                     /locus_tag="ACHJ8C_000311"
     CDS             complement(56824..57744)
                     /gene="malM"
                     /locus_tag="ACHJ8C_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO5550231.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGIRGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(58066..59406)
                     /gene="lamB"
                     /locus_tag="ACHJ8C_000312"
     CDS             complement(58066..59406)
                     /gene="lamB"
                     /locus_tag="ACHJ8C_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO5550232.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGSSSTNPYYGKAVSADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(59478..60593)
                     /gene="malK"
                     /locus_tag="ACHJ8C_000313"
     CDS             complement(59478..60593)
                     /gene="malK"
                     /locus_tag="ACHJ8C_000313"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO5550233.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQQVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            60958..62148
                     /gene="malE"
                     /locus_tag="ACHJ8C_000314"
     CDS             60958..62148
                     /gene="malE"
                     /locus_tag="ACHJ8C_000314"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO5550234.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYQ
                     DELAKDPRIAATMDNAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDAALKDAQT
                     RITK"
     gene            62302..63846
                     /gene="malF"
                     /locus_tag="ACHJ8C_000315"
     CDS             62302..63846
                     /gene="malF"
                     /locus_tag="ACHJ8C_000315"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO5550235.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKIYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ESATQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            63861..64751
                     /gene="malG"
                     /locus_tag="ACHJ8C_000316"
     CDS             63861..64751
                     /gene="malG"
                     /locus_tag="ACHJ8C_000316"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO5550236.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(64845..65255)
                     /gene="psiE"
                     /locus_tag="ACHJ8C_000317"
     CDS             complement(64845..65255)
                     /gene="psiE"
                     /locus_tag="ACHJ8C_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO5550237.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            65470..65748
                     /locus_tag="ACHJ8C_000318"
     CDS             65470..65748
                     /locus_tag="ACHJ8C_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5550238.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(65795..67891)
                     /gene="yjbH"
                     /locus_tag="ACHJ8C_000319"
     CDS             complement(65795..67891)
                     /gene="yjbH"
                     /locus_tag="ACHJ8C_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO5550239.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPALIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDPREGSIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGVGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(67891..68628)
                     /gene="yjbG"
                     /locus_tag="ACHJ8C_000320"
     CDS             complement(67891..68628)
                     /gene="yjbG"
                     /locus_tag="ACHJ8C_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO5550240.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGTPPSTVTLFGLISRPGKQS
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(68625..69263)
                     /gene="yjbF"
                     /locus_tag="ACHJ8C_000321"
     CDS             complement(68625..69263)
                     /gene="yjbF"
                     /locus_tag="ACHJ8C_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO5550241.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHARLVKTLLGGDNLI
                     EVNNLATDPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(69378..69620)
                     /gene="yjbE"
                     /locus_tag="ACHJ8C_000322"
     CDS             complement(69378..69620)
                     /gene="yjbE"
                     /locus_tag="ACHJ8C_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO5550242.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
BASE COUNT        17337 a        18817 c        17429 g        16151 t
ORIGIN      
        1 ttatcacttt gatgttgttt ttgacattct ttgcccacgc gaacgactcc gagcctggca
       61 gccagtattt aaaggcagca gaggccggag accgacgcgc acaatatttt cttgccgaca
      121 gctggtttag ctccggcgat ttgagcaaag ccgaatattg ggcacagaaa gccgccgaca
      181 gcggtgatgc cgatgcctgc gcgctgttgg cgcagatcaa aatcaccaat ccggtcagtc
      241 tggactatcc acaagcaaaa gtgcttgccg agaaagcagc gcaagcgggc agtaaagaag
      301 gtgaagtaac gctggcgcat attctggtaa atacgcaagc gggtaaaccg gattatccaa
      361 aggcgatttc gctgttagaa aacgcctcgg aagatctgga gaacgactct gccgtcgatg
      421 cccaaatgct gcttggtttg atttacgcca acggcgtggg cattaaggcc gacgatgaca
      481 aagcaacctg gtatttcaaa cgtagctccg cgatttcccg aaccggttat tccgaatact
      541 gggcgggaat gatgttctta aacggtgaag agggttttat cgagaagaac aaacaaaagg
      601 cgctgcactg gttgaacctg agctgtatgg aagggtttga taccgggtgt gaagagtttg
      661 aaaaattaac caacggttaa ggtaggcgca gcaaacgtcg catcaggcgt tgatgtcgga
      721 tgcggcgtaa acgccttatc cgacctacaa aatcacgcta catcaggcta ttgcgctgtc
      781 tgacgtaggc ctgataagac gcggcaagcg tcgcatcagg cgttgatgtc ggatgcggcg
      841 taaacgcctt atccgaccta caaaatcacg ctacatcagg ctattacgct gtctggcgta
      901 ggcctgataa gacgcggcaa gcgtcgcatc aggcgttgat gtcggatgcg gcgtaaacgc
      961 cttatccgac ctacaaaaac cacgctacat caggctattg cgctgtctgg cgtaggcctg
     1021 ataagacgcg gcaagcgtcg catcaggcgt tgatgtcgga tgcggcgtaa acgccttatc
     1081 cgacctacaa aatcacgcta catcaggcta ttgcgctgtc tgacgtaggc ctgataagac
     1141 acgtcagcat cgcatcagac attgatgccg gatgcggcgt aaacgcctta tccgacctac
     1201 aaaatcacgc tacatcaggc tattgcgctg tctggcgtag gcctgataag acgcggcaag
     1261 cgtcgcatca ggcgttgatg tcggatgcgg cgtaaacgcc ttatccgacc tacaaaatca
     1321 cgctacatca ggctattacg ctgtctggcg taggcctgat aagacgcgtt aagcgtcgca
     1381 tcaggcgttg atgtcggatg tggcgtaaac gccttatccg acctacaaaa accacgctac
     1441 atcaggctat tacgctgtct gacgtaggcc tgataagacg cggcaagcgt cgcatcaggc
     1501 gttgatgtcg gatgcggcgt aaacgcctta tccgacctac aaaaatcacg ctacatcagg
     1561 ctattactca gtcaggcatc cacacattgc cgggtggata tccccccggc aatcttcaat
     1621 tactgatccg cagtcttatc aaatttgccc agcacttcac gctcataagc cagcgctttc
     1681 ttacggtcaa atttgtgttc ccacttggca atcaccagca ccgccagcgc attacccacc
     1741 acgttcagcg cagtacgcgc catatcgagg atacggtcaa caccggcaat aaacgccaga
     1801 ccttccagcg gaatacctac gctacccagc gttgccagca gcaccacaaa cgacacgccc
     1861 ggcacgccag caatcccttt cgaggtcacc atcagcgtca gcaccagaat gatttcctgc
     1921 cagatggaga ggtcaatgcc atacagctga gcgatgaaaa tagcggcaat actttgatac
     1981 agcgtcgaac catcaaggtt aaaagagtaa ccggtcggca ccacgaaact ggtgatcgac
     2041 gccggtgctc cgtaggcttc catcttctca ataattcgcg gcagcacgct ttcagagctg
     2101 gcagtggagt acgccagaat cagctcatct ttcagaatac gaatcaggat ccagacgctt
     2161 aacccgcaca ggcgcgccac aattcccagc actaccagcg cgaagaacag aatggcgaaa
     2221 tgcaccagca gcaccagttt cgccagtggc cacagagacg agaaaccaaa gttagccacc
     2281 gtcaccgcaa tcagcgcaaa cacacccacc ggcgcataac gcatcaccat gtgagtcact
     2341 ttaaacatgg tttccgagat ggagcggaaa actgtcacca gcggttcacg atgcgtcgcg
     2401 ggcagggatg aaagccccag accaaacagc accgagaaga agatgatcgg cagcatttcg
     2461 cctttcgcca tcgacgccac gatgttcgtc ggcaccagcg acaaaatcgt gcccataatg
     2521 ccgtgggaac tgctttgtac cgcttccgtg gtgctctgat atttcgagat atcgacggtc
     2581 gccagctgcg acatatccac cccggcaccg ggctggaaga cgttcgccag agtgatcccc
     2641 aaaatgatgg cgacggtggt gatcacctcg aagtagataa tggttttcgc accaatacgc
     2701 ccgagctgtt tggcatcacc aacacccgcg atacccacca ccagcgtgga gatcacaatc
     2761 ggcacaacaa tcattttgat cagatggatg aagatatcac ccgccggaga gagcaaattg
     2821 acgaccagcc agtcgcggct gtcgctatgg tagtgcaggt agcttcccag gagaatgccc
     2881 agcaccatag caaacagaat ctgccaggcc aggctgaatt ttatattttt catcagaata
     2941 acttcctcaa tggagcgccg ttccccgttc agaaacgcga gaacgtgaca tactgccagg
     3001 gcatgaattg tgttgcgttg gtttgtagat ttttttaacg cttatgatca gtatcatttg
     3061 cgcggtatgt tgcgcaaccc tttaacaaca gagcttttcg ctgcattaat gcataataac
     3121 gcgagtttat ggcaggatta atgaataacc atttgttata gaaggatttt tatttgttta
     3181 atctgcaata atactgatta gtgattattt ttgattatat gttcattcga ttatttttta
     3241 tgcagctcaa taacataatc ttcatcacca gatgattaat ctgatctccg ctgcaacaat
     3301 ttcgccatat taatcgactc gaccagctgt gtccggttga tccggggtga gttgagatcc
     3361 atcacttttt gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa
     3421 gccagcagca tcagagcggt gatttcgtta gagtccagcg cgagggcttt gtcgatcatt
     3481 gcgcgagcct ggacggtcat atgttggctg gcctggtaat acagcaccgt cgccagcgcc
     3541 gcatacagtt cagcgttctc accacgtagt tgcaacgcct gacggtacgc cagcagcgaa
     3601 ttgctgtaat cgttttgcca cagataatac tcgcccagta acgcccactg ttcgctgttt
     3661 tgtggattag cacggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg
     3721 ctggcaaact gatgcagcgg atcgcgctga cgctgatact ccgcacgtac cgcctgccat
     3781 tttggactta acagataact gccgacacag agaaatacca taaaaatcgt gagtgttgtc
     3841 agcattttca ccggtatttg gggctgtttc atcgcttcgc cctcactcgc cagaggatca
     3901 gtgtcatcag cagtaacaac actactggca gcgcccacaa caccagcgtc tgacccgtta
     3961 acggtgggtt atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga
     4021 cttcactttt cccctcttcc accatggtgt aaacctgatg gcgcatactg actgccaccg
     4081 gcgcattgga ttccagtaag ttctgattct ggcactgcgg acaacgtaac tggctggcaa
     4141 tatttaacgc ctgttcctgt tgttgcggat tggcgaattg ccaggtgtct acaacctgag
     4201 cgcgcgcaaa acaggtaaat aacagcagta gcgtgagaag ccctttattc atcgcgcttc
     4261 cttccccgcc atccgcttaa caatgcgccc gcaatcatca acaatcctcc cccccagatc
     4321 cagcgcacac cgctttgtac atacagacga aaagcgtaac gatccgctcc ggttttctcg
     4381 cccatgaccg cataccagtc atggatgcca ttccagcgaa ttgacggttc catcatttgc
     4441 tgacgacgtg cttcgtaaaa acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg
     4501 tcaaacagtg ccactatcgc tttttcgctg gtgtaattgc ctttggcttg cagatcgagg
     4561 cgctcaaaac ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg
     4621 ctgatctcct gacggctgac gctggaaacc acaatccccg cggcaaataa cagcacgccc
     4681 gcatgcgcca gcagcgccgg aagctgagca cgtttgccag agacaaacgt cgccagcaca
     4741 atcaccacca gcatcaacag agcaaacggt aacgtcgcgc ggttaaaata cggcgcgcca
     4801 acggagaggc gtccccagcc cagcaggccg taaatcatcg gataaagcgt tcccaccagc
     4861 acgatcagta gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat
     4921 aaccccgaaa aacgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaga
     4981 gacgcaaggc taatcagtgc aaacaggctg aacaacggca cggcgcggac gttatccagc
     5041 gcgaacgcat gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac
     5101 agcatcagag ttactatcgc cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt
     5161 gtcagggata aactgtgcag cagcgctgtg gcagaaagcc agggtaataa cgacgcgttt
     5221 tccaccggat cccagaacca ccagccgccc cagcccagtt cgcaataggc ccaccaggaa
     5281 ccgaggatga tccccgccgt taatgcgctc cagccaagta gtgcccaacg ccagcaaatt
     5341 cgggcgcagg taccatcaaa ctcgccgcgc agtaaactcg ccagcgccac gctcgccgcc
     5401 accatcaaac cgccgtagcc gagataaagc agcggtggat gaaagataag accgggatgt
     5461 tgcagcatcg gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga
     5521 tcggaccaca gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctagc
     5581 gtcagcggaa atagcggatc ggtttgctgt cgataatgcc aggcaaacag cgcgctccag
     5641 gcggaaagca gcagcaccca aagcagcagc gaaccttcat gaccgcccca caccgccgcc
     5701 agtttgagtt cccacgacag caggctatag ctatgttggg cgacataaat gaccgagaaa
     5761 tcgctgatga gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc
     5821 gccagaatgc cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgcg
     5881 aaggccgtca acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg
     5941 acttcaggaa ggaatacgtc cagaaaccac ctcttttctg gcttacgcca ccgtcaactg
     6001 cccggcgtag agaatgaaaa agcgcagcat caacacgccg accaggctgg ccccacacgc
     6061 agccagcacg gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac
     6121 aatcagcccc agcccggcga caccaagcca gaaccaccac gtccagaaac cgccgcccaa
     6181 tgccgccacc agcgcacgca ctttaccgtc atcgcccagc gccagaccga caaaaaacgc
     6241 caccagcagg aagatttcgc cccataccac agggatctcc atacggtgta caaagtgcgc
     6301 ttccgtggaa tgcggatttt tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc
     6361 accggacgag atgccggaga agaggaacag caccggcagg atcgggttat tgaggaacgg
     6421 atacgatttc agcgccgaca gcagaaagcc ggtataagcc cccagcagca ccgccagcac
     6481 cagcatcaat gtttccagtc ctcgatgaac gggcgtcagc aggctcagaa ccttttgcac
     6541 gatccccagc ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt
     6601 cgccagccac agcaccagca ccaccatgta aagctgaaac agcatcaccc ccatcgacat
     6661 caccgaggta aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg
     6721 gaagacgagg atcaacagac cgaggatcac cgcgccaggc ccgacaatca gcgtggtgcg
     6781 cagcaacgta ctgtctgcac cgcctgcctg agggtagaag cgacgtaaca gcacggccag
     6841 cgtcaccaga ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca
     6901 caccagcgat tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta
     6961 aacggtacgc ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtgggc
     7021 ttctggcgca gcagttgcga aatctcactg ctgggatcgt ccagattgcc aaacgtcagc
     7081 gccttcgtcg ggcaagcttc aacgcacgcg ggcagcttac cggcctgcaa atgggtctta
     7141 cggcagaaat cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg cacgcggtac
     7201 ggacaggcag cgatgcagta ctgacaaccg acacagagat ccggattaac atcgacaatc
     7261 ccactggcag cgtcgcgaaa agacgctccg gtcgggcaga cgtcaacgca cggcgcatga
     7321 tcgcagtgct ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc
     7381 ggctcgctac gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga
     7441 caagcatcca tacaggcggt acagccgatg cataacgact cgtcatgcac catgccgtaa
     7501 cgcacaccat tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggcg
     7561 gccagcacac cgacgccggt gagaaactgg cgacgtgacc aggtcatggc tgctccttaa
     7621 gcaacggaac agacgccggg ttaaagttcg gattggtgcg ttgatcgctg tggcaatcga
     7681 cgcaaatctt aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca
     7741 gggaatggca gctggcgcat gccactttgg ttacatgaac atcgtgcggc cagaacgctt
     7801 tttgcaactg ttccggcaga tggcaggaca tacaaacact gttctgctct ccaaccttgt
     7861 acatcggctc gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatggct
     7921 gaccgtggca gttggtgcag gtgaccggca gtttgttatt cgggttgata acggaagcat
     7981 gtttgccatg cataccttcg gtatctggct tatgacagtc cagacaggcg gcatccgggt
     8041 tacgctgctg ggtaacttcg tagcgatcac cagatgcctg cgccgcaggg gtagcggtaa
     8101 ttccgttcag gctccacaac aggcccgacg ccagcacccc ggcagttaat aacgaacgta
     8161 atacgctcat attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg
     8221 gcttaacaga ccgtttttac gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa
     8281 gtcctgcttc tcggccttga tttgctccat gttcaggcca atggcctgct gggctttctc
     8341 tttggtcgag atatccggga tctggatttc atgggtaatg cctttggtcg ccagcaggcg
     8401 cgccagtttg gtgcgtgcat ccgccgcttt atccatcgcc gtaccgagca tcctcagacc
     8461 ttcttccggt gcgtgcatat gaatgccgtg ggaagcgatc gccaaatccc aacgccactg
     8521 ggcatgacgg atatcgtcct gaatcggctt catttcggct tccgttgcgc ctgcatccag
     8581 cgccgctttc gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc
     8641 gttaatcgac tgcttacgtt ccgcgaccac tttttgcaag gcagctttgt cctgggtatg
     8701 gcagttcgca caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtgta
     8761 gagtttgcct tcggcgttct gcacttttgg catatggcaa tcgatacagg tcacgttgtt
     8821 tttgccgtga atgcccgctg tccaggtttc atattccggg tgctgcgctt tcagcattgg
     8881 cgttttcgac agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat
     8941 attttcgact ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa
     9001 gtaatactcc acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt
     9061 ctcaaaaggt ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa
     9121 ctccggtttg cctttggcga actctggtga ggcggtgtta tggcaatcgg cacaacctaa
     9181 gttgttgacg atttccgggc cgccgcgcgc ccattttccg tggaagtagc catcttcgcc
     9241 gtctttctgg atcagacgcg ccacatccgg gcttttacaa ctccagcacg ccatcggtag
     9301 cggaccatct tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca catcggtcac
     9361 agcaaaggca tgtccacgcg gcttgttgta atcgcgcgag aagggatacc ctgcccacag
     9421 gatcaccagc cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtagc
     9481 tttccaggag agatattgat cgggatgctg cggggcaaag gtttcattct tcgcttccac
     9541 agttacaggt gttgcgggag cagccgtttg ttcagcgtga acagaagtga aaaagaaaaa
     9601 aggaatcaat aagctgaaga tacggcgtgc gtttattttt atccttgtca tagggcttca
     9661 tccgaattgc gccattgttg caatggcggt ttttattgtt tttcacgaca gtaaccgcac
     9721 ctacactgtc atgacattgc tcgtccctat gtgtaacaaa taaccacact atgaatgttg
     9781 tctttaatca attgtaagtg catgtaaaat accactttag agttagtcag tatcttcctc
     9841 tttttcaaca gcatgcataa ctgcatgttc ctcaaagaat taatcaactt ttgttgctga
     9901 ccttcaaaaa ttaccctgcc gtttatttgc acaattctac ttttgcgtga tctgtcgccc
     9961 aaatactaaa caaaactgtc aataccccta catttaacgc ttatgccaca tattattaac
    10021 atcttacaag gagaacaaaa gcatgagtca aattcacaaa cacaccattc ctgccaacat
    10081 cgcagaccgt tgcctgataa accctcagca gtacgaggcg atgtatcaac aatctattaa
    10141 cgcaccagat accttctggg gcgaacaggg aaaaattctc gactggatca caccgtacca
    10201 gaaggtgaaa aacacctcct ttgcccccgg taatgtgtcc attaaatggt acgaggacgg
    10261 cacgctgaat ctggcggcaa actgccttga ccgccatctg caagaaaacg gcgatcgtac
    10321 cgccatcatc tgggaaggcg acgacgccag ccagagcaaa catattagct ataaagagct
    10381 gcaccgcgac gtctgccgct tcgccaatac cctgctcgaa ctgggcatta aaaaaggtga
    10441 cgtagttgcg atttatatgc cgatggtacc ggaagccgcg gtcgcgatgc tggcctgcgc
    10501 ccgcattggc gcggtacatt cggtgatttt cggcggcttc tcgccggaag ccgttgccgg
    10561 acgcattatc gactccagtt cacgactggt gatcacttcc gacgaaggtg tgcgtgccgg
    10621 gcgtagtatt ccgctgaaga aaaacgttga tgacgcgctg aaaaacccga acgtcaccag
    10681 cgtagagcat gtggtggtac tgaagcgtac tggtggaaaa attgactggc aggaagggcg
    10741 cgacctgtgg tggcacgacc tggttgagca agccagcgct cagcaccagg cggaagagat
    10801 gaacgccgaa gatccgctgt ttattctcta tacctccggt tctaccggta agccaaaagg
    10861 cgtgctgcac actaccggcg gttatctggt gtacgcggcg ctgaccttta aatatgtctt
    10921 tgattatcat ccgggcgata tctactggtg caccgccgat gtgggctggg ttaccggaca
    10981 cagttatttg ctgtatggcc cgctggcctg cggcgcgacc acgctgatgt ttgaaggcgt
    11041 accgaactgg ccgacgcctg cccgtatggc gcaagtggtg gacaagcatc aggtcaatat
    11101 tctctatacc gcgcccacgg cgattcgcgc gctgatggcg gaaggcgata aagcgatcga
    11161 aggcaccgac cgttcgtcgc tgcgcattct cggttccgtg ggcgagccaa ttaacccgga
    11221 agcgtgggag tggtactgga agaagatcgg caacgagaaa tgcccggtgg tcgacacctg
    11281 gtggcagacc gaaactggcg gtttcatgat cacgccactg cctggcgcta ccgagctgaa
    11341 agccggttcg gcaacacgtc cgttcttcgg cgtgcaacca gcgctggtcg ataacgaagg
    11401 taacccgctg gagggggcca ccgaaggcag tctggtgatc accgactcct ggccgggtca
    11461 ggcgcgtacg ctgtttggcg atcacgaacg ttttgagcag acctacttct ccaccttcaa
    11521 aaatatgtat ttcagcggcg acggcgcgcg tcgcgatgaa gatggctatt actggatcac
    11581 cgggcgcgtt gacgacgtgc tgaacgtctc cggtcaccgc ctggggacgg cagagattga
    11641 gtcggcgctg gtggcgcatc cgaaaattgc cgaagccgct gtggtcggta ttccgcacaa
    11701 tattaaaggt caggcgatct acgcctacgt cacgcttaat cacggggagg aaccgtcacc
    11761 agaactgtac gcagaagtcc gcaactgggt gcgtaaagag attggcccgc tggcgacgcc
    11821 agacgtgctg cactggaccg actccctgcc taaaacccgc tccggcaaaa ttatgcgccg
    11881 tattctgcgc aaaattgcgg cgggcgatac cagcaacctg ggcgatacct cgacgcttgc
    11941 cgatcctggc gtagtcgaga agctgcttga agagaagcag gccatcgcga tgccatcgta
    12001 acccacaatt gccggatgcg ctccgggcaa caagtcggca acggctgtag gcctgataag
    12061 acgcgacagc gtcgcatcag gcattgattg ccggatgcgg cgtataacgc cttatccggc
    12121 ctacattcgg caagggttac ccaagcgtta accttctccc ataagggagc gggaattaaa
    12181 acaatcccta cattacctct ggagaatctg tgatgaatgg tacgatttat cagcggatag
    12241 aagacaatgc gcatttcagg gagttagtcg aaaaacggca acggtttgcc accatcctgt
    12301 cgattattat gctggcagtt tatatcggct ttattttact gatcgccttc gcgcccggct
    12361 ggctgggcac cccgctgaat ccgaacacca gcgtcacacg cggtattcca gtcggtgtcg
    12421 gggtaattgt gatctccttt gttctcactg gtatctacat ctggcgggcg aacggcgaat
    12481 tcgaccgtct taataacgaa gtcctgcatg aggtacaagc atcatgaaaa gagttctgac
    12541 ggcgcttgcc gccacactcc ctttcgcagc taacgccgcg gatgctatta gcggggccgt
    12601 agagcgtcag ccaacgaact ggcaggcgat tattatgttc ctgattttcg tcgtgtttac
    12661 gctcggcatt acctactggg cgtcaaaacg cgtacgttcg cgtaatgact actacaccgc
    12721 aggcggcaat atcaccggtt ttcagaacgg gctggcgatt gccggggact atatgtccgc
    12781 cgcctcattc ttggggatct ccgcgctggt gtttacctcc ggctatgacg gcttaattta
    12841 ctcgctgggc ttcctggtgg gctggccgat cattttgttc ctgattgccg aacgtctgcg
    12901 taacctgggg cgctacacct ttgccgatgt agcctcttac cgtctgaaac aagggccgat
    12961 tcgtattctt tcagcctgtg gttctctggt ggtggtggcg ctttacctta tcgcccagat
    13021 ggtgggcgca ggtaaactga tcgagctgct gtttggcctt aactatcaca ttgcggtagt
    13081 gctggtcggc gtgctgatga tgatgtacgt cctgttcggc ggcatgctgg cgaccacctg
    13141 ggtacaaatt attaaagccg tgctgttgct gttcggtgcc agctttatgg cctttatggt
    13201 gatgaaacac gtcggcttta gcttcaacaa tctgttcagc gaagcgatgg cggtacaccc
    13261 gaaaggtgtc gacatcatga agccgggcgg gctggtgaaa gatcctatct ccgcgctctc
    13321 tttggggcta ggactgatgt ttggtacggc gggcttgccg catattctga tgcgcttctt
    13381 tacggtcagc gatgcccgcg aagcacgtaa gagcgtgttc tacgccaccg gatttatggg
    13441 ctacttctac atcctgacct ttattatcgg ctttggcgcg atcatgctgg tcggtgcgaa
    13501 tccggaatat aaagacgcgg cgggccatct gattggtggt aacaacatgg cagcagttca
    13561 cctggccaat gcggtgggcg gcaacctgtt cctcggcttt atttcagcgg ttgctttcgc
    13621 caccattctt gcggtggttg cgggtctgac gctggcgggc gcatcggcgg tgtctcacga
    13681 cctgtacgct aacgtcttca aaaaaggtgc gaccgaacgt gaagagctgc gggtatcgaa
    13741 aatcaccgta ctgatcctcg gtgtgattgc cattatcctc ggcgtgctgt ttgagaatca
    13801 gaacatcgcc tttatggtgg ggctggcgtt tgccatcgcg gcgagctgta acttcccgat
    13861 cattctgctt tccatgtact ggtcgaaact gactacccgt ggcgcaatgc tgggcggctg
    13921 gttaggactg attactgcgg tggtgctgat gatcctcggt ccgacgattt gggtacagat
    13981 cctcggtcac gaaaaagcca ttttcccgta tgaatacccg gcgctgttct ctatcagcgt
    14041 ggcattcctc ggtatctggt tcttctcggc aacggataat tcagcggaag gtgcgcgcga
    14101 gcgtgaactg ttccgtgcgc agtttatccg ctcccagact ggctttggcg ttgagcaagg
    14161 ccgcgcgcat taatctctcc ccttccccgg tcgcttgatc ggggaatttc ttcagaacat
    14221 catcctggcc accagcggcg aaaccaatac catcaataca cctgaaagca tcatgaccag
    14281 gcttgcaact acgccttctt gttgacccag ttcatacgac cgcgcagtcc cggcaccatg
    14341 agatgccgcg ccgaatccag cacctttcgc catcccttcg cgaatagaga gacgtaaaaa
    14401 caacacgtcg ccaatcgcca tgccaaacac accggtgacc accacaaaca gcgccaccag
    14461 atcaggctgt ccgccgagcg actggctcgc tgccaaagca aatggcgtag tgaccgaacg
    14521 caccgccagg ctacgctgga tctcatctgg cagggtaaac aatcgcgcca gccacactga
    14581 gctggtcact gccaccaccg ttgccgtcag tacgcctgcc gtcaacgaca tccagtgacg
    14641 cttaatgatc gcccgattgt catacactgg taccgcaaag gcgatggtgg ccggacctaa
    14701 tagccatagt aaccagtgcg cttcgccaat atagttttgc caggaaatat tgccaaaaac
    14761 cagcagcata accagtagcg taggcgtcag caccagaggc atcaatggca atttacgaaa
    14821 gcggcgataa aggcgtttgt taagaaaata gataactagc gtaattgcca gacagagcat
    14881 acttagctga aaattagcca cggtttaacc tgcgcatttc atagcgatag actttttcca
    14941 ctacccaggc agtcgtgccc agcaccatca ccgtactcag cgcgatcaca gcaaaaatac
    15001 gccagccatc caccagtaat agttgtgcat agttcaccac agccaccacg gcagggacaa
    15061 aaaacagcaa catttcggcc aatagccaac gcgccccggc tcgaacccat tgcagaggaa
    15121 gaatgcgaca aacaatcagc gccagcatca ggatcatccc caccaggttg gcaggcagcg
    15181 gcaaatgcag ccaggagacg agatactgcg caaaaataaa taaacctgca tacagcacaa
    15241 cctgaaaaaa tacctggata gattgcacta cggcaggggt aatgcggcga agcgccacgg
    15301 gcatgagagg atatcctgaa aataaaacag aatggtaagt atagagagcg ctcgcccctg
    15361 actgaaatga attaaaatca ttgcaggtat agtcaaaggg aatagttatg gatatcagaa
    15421 cgctgcgtta ttttgtcgaa gtcgtgcgcc agcaaagctt cacccgcgca gcggagaagt
    15481 tgtttgtcac ccaaccaact atcagcaaga tgctgaagaa tctcgaagat gagctgaact
    15541 gtatattgtt gatccgcgat ggtcgtcggc tgcttctcac tgatactggt cgcgtcgtat
    15601 ttgagcgcgg gctggcttta ctggctgagt ttcggcaact ggaagctgag cttagcgata
    15661 tcaatcagct taaaaccggc gttttgcggc tgggaatccc gccgatggtt gggatgttga
    15721 tggccgggcc gattggcctt tttcgccagc gttaccccgg cgtagaattg aaaatttcgg
    15781 agtttggtgg tctgacggta caacaagcgg tgatgaacgg tgaactggat atggcactga
    15841 cagccctgcc agtagaagaa agcggcctga caacgttatc gctgtttagt catccactat
    15901 gtgtactgac gccacattca ggtaaatggc ttgaatgtga tgccgtctca ccggagacat
    15961 tggcggaaca tcctctactt atctataacg aagattttgc cttaagtaag caattgatga
    16021 agttattcga tgaacatggc gttaagccga gaattgcagt tcgcagcggg caatgggatt
    16081 ttctcgccgc gatggttcag gccggtgtcg gaatagccat attgccggaa cccatttgcc
    16141 agcgattaga taaaaacacc ttaaaatggc taccgctgga aagcgaacta agctggaaat
    16201 taggaatgat ttggcgtgag ggcgtttacc tttcccactc tgcccacgcg tggctgagct
    16261 gttgtgaagg atactggtta acgccagagt aagtcgggtt ggcgggctcg cccgccaacc
    16321 atgcagatta ctgattttcc tcaatcagca acgcttcaag caaatcgaga tcgtgcagta
    16381 atttttgcag cgtttcgtta ctgatctccc gcgtggcgcg caggtggtac agttcagcac
    16441 gttcagaacg caatgccgcc agacggaagc gacgctccag gttctcttcc aggattgaac
    16501 tttctacatc attacgtcca tcggcgcgac gacgcaggtt accaatgacg cgagaactaa
    16561 cctctgtgag cagctggtta tcgatattct cttcggtatc cgccgccaga cgctcctcca
    16621 ttttctggat cgccacaatc gccacttctg ccgttgccgc tcgtgcaata cgttcctctt
    16681 tcaattgttg cgaatggtcc gcgacttcta tgtgttgtag cagaataggc aacatcacca
    16741 cgccgacaaa caatgagaag agaatgacgc cagccgccag gaacactaac tcatagcgcg
    16801 cagggaagac gttaccatcc ggcaagagaa gcgggataga gagcacaccg gccagagtga
    16861 tcgccccacg caccccggcg aaagacgcga tcaggatttc tcgtgtagtc cacgaaccaa
    16921 actccatcgg cttcttcttc aggaagcggt tgctgaactt tttcatcgtc cacagccagc
    16981 cgaaacggac cagcatcagc gccgcatata tcagaataat atcggtaaac agcatccaga
    17041 tttcgacgtt agggtcgatt tctgccgcca tcagcgacgt ctccagaatc cccggcaact
    17101 gcagacctaa cagcaggaac accatgccgt taaagacaaa ttccagcatc gcccaggtgc
    17161 tgtttgcgcg caggcgcatt gccagcggcg cacggcgcat cacaccggag cgggtgatgg
    17221 tcatcccggc ggcaaccgca gcgaggatgc cggaaacgcc aatatgttcc gcaatcagat
    17281 aagaagcgaa tggcagcaag aacagcagaa cgatctgcgt cgcgggttca tcaccgcccc
    17341 agcggctgag gaatcgcagc gagcgaccat acagccagct caccacaaaa ccggcgagaa
    17401 taccgcctat tgccactttc ataaattcga ccgtcgcacc gccgacggtg aagatcattg
    17461 tccccatcgc cactgccacg gcaaacttca acgacaccag gccggaggcg tcattcatca
    17521 acgcttcgcc ctgcaaaatg cccatgattt ttttcgggat gcgcccctcg ccaacaatcc
    17581 cggagagcgc tacagcatcg gtcggagaaa gcaccgcagc cagcgcaaag gcggggatca
    17641 gcggaatgcc cggcaccacc cagtaaataa ggaagccaat gccgactacg gtgaccacca
    17701 ccagcgccag cgcgaggccg aaaatctctc gcccatgttc gagaaattca cgggtcggcg
    17761 ttttccagcc atcagcgaac agcaacggcg ggataaacaa gactaaaaag agttcaggat
    17821 caaactccac atgcaaacca aacgtcggcc acgccagtag cgcaccgatg gcgatttgca
    17881 taagcggaag cgggatctga aagggcatga cacgagtgac caccccggac agcgagacca
    17941 cgagggtcat tatcagtatg gtgaagaaga tttccatgcg ttccctgttt gcgatgtttt
    18001 ttatgatctg agaaaggcgt tatgcctgaa ttctcatttt ttcagaatat cgcaaaagct
    18061 cacacaatgt gcggagaaaa taaaaacggg cagccattgc cacccgtttt cagaatccta
    18121 tgtaagatta gatagcccag ccacccgcat agaaggtcac cagcgccacg gcaataacga
    18181 ccgtaccgat gttcaacttg cgccattcgc cggaaaccag acgaccaatc accagagtcg
    18241 cgaagccgat catgatgcct gttacgatgt tacaggtcag cacgatgaat accgccgtaa
    18301 ccagacccgc catcgcatca acaaaatcag caaagtcgat tttcgccacg ttgctcagca
    18361 tcagcaggcc aacgtacatc agcgccggag ccgttgcgta ccccggaacg aggtaagaga
    18421 gcggagagag gaacagaatc agcaggaaca gcacgccaac ggtgatagcc gtcaaaccgg
    18481 ttttaccgcc cgccgccgta cccgccgcag actcgatgta taccgctgcc ggagctgcac
    18541 ccaccaggcc agaaaaaacg ctgctcatgg agtcggtggt cagtgctttc ccgccgtcga
    18601 tgatctgccc atctttatcc agcaggttcg cctgaccggc gacggcacgg atagttccgg
    18661 tggcatcaaa tactgctgtc atcaccagtg ccagaacact tggcaggact acaggattca
    18721 gcgcgcccat aatgtccagg ctgccaatca gtgaattgcc gttttcatcg ctcaatgaag
    18781 gcatggcaaa aacgccggag aaatggacgt taggatcgaa gatcaaaccg acaattgaga
    18841 taccgataat ggtcagcaga atgccaccag ggactttcag tttttccagg ccgataatca
    18901 ctgccagacc caccaacgac atcatcaccg gaaaggtcgt gaaatccccc agcgcgacgg
    18961 gcagaccatc aagtgggttt ttaatcacca gaccgacacc attagcagca atcagcagca
    19021 ggaacagacc gataccaatc cccgtgccgt gcgccacacc gtgcggcaag ttgcgcaaaa
    19081 tccagctacg gatacccgtg gcagaaatta ccgtaaacaa cacacccatc aggaacacgg
    19141 cacccagcgc gacaggtacg ctaatatgtt gccccagcac caaactgaat gcggtaaacg
    19201 ccgtcagaga gatggcgcaa ccaatcgcca acggcagatt agcccacaga cccatcacga
    19261 tagaaccgag tccggcaacc agacaggttg caacgaaaac tgccgcaggc gggaagcccg
    19321 ctttacccaa catgcctgga acgacgataa ctgagtagac catcgccaga aacgttgtta
    19381 acccggcaac cacttcctga cggacagtgc ttccacgttg tgaaatttta aaccaggcgt
    19441 cgagtgaacc gccggtacgc gctgatggcg tagacataag aaaacatccc ctgagagttt
    19501 aattttcgtc aagatgcgtg gcggacaatc ccctgccagc gaaacgttat cttcctgctc
    19561 caggtttgcg acaatttgct gcgtcgcaaa aaaaagcaaa cgtttagctc cgcgcataca
    19621 aaacgggtgg taaaaaaagc aaacgattat ccggcgatca tagcggcttt ttcaaccgaa
    19681 gtttgctgct tttttctctt ttattgtctt gacgatgaaa aaccaggcaa ttgatgcgca
    19741 aacgttatcg tcgatgcgca agattgcagc gttatcgagc cgtataccgt tcaggattgc
    19801 gataaaaaat cgtctgagat gacaaaaata ttgcggctaa aaagtgtact tttacactgg
    19861 cgaagaaacg ccggtcacat acaattaaag catcaacacc aaccggaacc tccaccacgt
    19921 gctcgaatga ggtgtgttga cgtcggggga aaccctcctg tgtaccagcg ggatagagag
    19981 aaagacaaag accggaaaac aaactaaagc gcccttgtgg cgctttagtt ttgttcatct
    20041 tccagtaagc gtgcgccggt accttcttct cctaagcggt cgcccgggtt acgcaacggg
    20101 caatcactgc gcgaaaggca gccacaacca atacatccat ccagttcgtc acgcagcgcc
    20161 actaaggtat gaatgcgccg ctccaactct tctcgccatt gggacgaaag ctgtttccac
    20221 tctttcgcac ttaacgtatg cccttctggc aacacgccaa acgcttcacg aatggttgcg
    20281 agcggaatgc caatacgctg agcaattttg ataattgcga catatcgcaa cacatcacgt
    20341 ttatatcgcc gctgattgcc gctattacgg atactggtaa tcaacccttt gctttcatag
    20401 aaatgcagcg ccgataccgc cacaccgctg cgtttcgcca cttcgccggg ggttagcagc
    20461 gctttaatgc ggggtaattt cttttccata aatcgcttta cctcaagtta acttgaggaa
    20521 ttatactccc caacagatga attaacgaac tgaacactga aaagaggcag atttatgtcc
    20581 catcagaaaa ttattcagga tcttatcgca tggattgacg agcatattga ccagccgctt
    20641 aacattgatg tcgtcgcaaa aaaatctggc tattcaaagt ggtacttgca acggatgttc
    20701 cgcacggtga cgcatcagac gcttggcgat tacattcgcc agcgccgtct gttactggcc
    20761 gccgttgagt tgcgcaccac cgagcgtccg atttttgata tcgcaatgga cctgggttat
    20821 gtctctcagc agaccttctc ccgcgttttc cgtcggcagt ttgatcgcac tcccagcgat
    20881 tatcgccacc gcctgtagtt ttattgcccg cgcgttaact cccgcgcggg taattgctcc
    20941 atccattgca taaacacctg cggcggcatc gccttcgcga agaaccatcc ctggcaatag
    21001 cgcacgccgc gtttgcgcag ccagttaacc tgcgcctcag tttcgacacc ttcagcgatc
    21061 gtttttaacc ccaggctgtg cgccagctcg atgatgtgtt ccgcaatcaa atgactggtt
    21121 ttgtgggtgg tcagcgtctc aacaaacgat ttatcgattt tcaaaatatc gacattcaat
    21181 gatttaaggt tatgcaagtt agagtagcca ataccaaaat catcaattgc cacttcgtaa
    21241 ccggccttgc ggaaagccag aataatcggc gtcattttgt cgacatcaag aaatgcatgt
    21301 tcagtcactt caaatttaat ttgctgcgga cgcaccgcat gttgctctgt tttctgattg
    21361 gttcgcgcta tcaaccgtga cgtatgaaaa tcggaggctg acaggttaat agaaacatag
    21421 cgatcggtat gtgttgccag atacgcgccc aaatcgcgga agacattatc aataacataa
    21481 tcagttacct gttcgatcat cccctccttt tctgccagcg gaataaactc tgccggattc
    21541 attacttgcc cctgctcacc aggccaacgt aacaacgctt cagcgccgat acatttttct
    21601 gttttgatat cgattattgg ctggtaataa aggcaaagtt gatgtttttc gagggcgcgt
    21661 tgcaatttgc gtttgggaga caaatagttt tgtcgaatac gtagccagag taatagcaga
    21721 acaagactcc ccaaaatacc ggcgggcaac gcaaaaataa gatgattata aaaatgggtt
    21781 ataagacgtt gatatgaagt cgcaacaatg gctgcaattg ggcgttttgt cgaataaact
    21841 gtcgcatata aatagccatt tcgttgtacg gttaaatcat tcaaatgaat cagcggagaa
    21901 aacgttgctg ccgaggcctc gttgcttaac gagaaaaagg ttttcgtcac cgtatcatac
    21961 acaccccatt gcaatgtcgg atcatcagac atcacttcac tccagaagag agggttgata
    22021 accgccacat aatttccccg ctgcatgtag gtcattttat agccagagaa aaaaggcgta
    22081 tcacggtaat aatagataga aacgttaggt tcgcgcttat aatcggccgg tgcaatcgta
    22141 tagccattta cagacgctat cagcgatgag cataaaaaat ggttatcacg agcataaatc
    22201 aattcattaa tataaagata gccacgaata atattcaaca ttcgcttttg atgggctgga
    22261 gtgcaaactt gcccctgata gcgttcagct gcatcgctca ctaaatctgc ctgacgaatg
    22321 accagctcag atttatccaa cgccagctga gcaaaagttc ggagctgatt attcacttct
    22381 gattttgccc aaaagaaggc aatccatagc gacagaatga tgggaaagag aactaaaaag
    22441 ataatgcccg gaaacgccag taattggtgt cgtgcacgat gactcattgc tatcaattcc
    22501 ctgcctgttt atacccggtt attgcaaggt gaaaccctgg ctaccgacgt ctttagataa
    22561 atcttacggt ataaatagca cttttgcctg atataaaatt gagacctttt taatcgtcac
    22621 caggatgacg ataattcatt gatcttaaaa attaatcacc ttcaggaatg cagaggcggc
    22681 gggaagatat acccttaatg ccgctgaaaa aacacataat ctgtacggca ataaaagtca
    22741 cggcctaatc tggctaacaa acaaccaatt gtgatatagt tcacaaaatt catgaaacaa
    22801 acagagtgtt tcatttttgt gttctgcaag cccccgcgat agccagagtg tatcacgcct
    22861 cccgtgaaca acgccgcgct gtccagggct tcagctcttt tccgaggaat tgtttgatgg
    22921 ctaccctcac cactggcgtg gttcttcttc gctggcaact tcttagtgcc gtaatgatgt
    22981 ttctggctag cacgctcaac atccgttttc gtcgggctga ttatgtcggg cttgcagtga
    23041 tcagcagcgg tctgggcgta gtttccgcct gctggttcgc aatggggttg cttggcatca
    23101 caatggcgga tatcaccgcc atctggcaca acatcgagtc ggtgatgata gaagagatga
    23161 atcagacacc gccacaatgg ccaatgattt tgacttgata tgtagaagcc tccataacgg
    23221 aggcttcttt tttatggctg gcagatgctt taatcatcca ccttaaaaca atataaccta
    23281 ttgttttaaa gacaaatcag aacggaatat cgtcatcaaa gtccatcggc ggctcgttag
    23341 acggcgctgc cggagcggac tgctgcgggc gagactgcgc gccgccgctg aactgattgc
    23401 caccctgcgg ctgctgaggc tgaccccaac cgccctgcgg ctgaccacca ccgatattgc
    23461 cacctgcagg agcgccacca ccctgacgac cacccaacat ctgcatggtg ccgccaacgt
    23521 tcaccacgac ttctgtggta tagcgatcct ggccggattg atcggtccat ttgcgggtac
    23581 gcagctgacc ttcaatataa acctgagaac ctttacgcag atattcgctg gccacttctg
    23641 ccagtttgcc gaacagcaca acgcggtgcc attcagtctg ctctttcatc tcgccggtcg
    23701 ctttatcacg ccaggattcg gaagtagcca gcgtaatgtt ggcaactgcg ccaccatttg
    23761 gcatgtagcg tacttccggg tcctgaccca gattaccaac gagaataacc ttgtttacgc
    23821 ctctgctggc catgttcgtg tctcctgaaa aaaaatcgtt ctgaataagt gtaaacgcgc
    23881 gattgtacca ttaccaatag cgcttttact atgttgtgac ctcggttccg ggaaacaaac
    23941 ctggccagac attgttacac aacactccgg gtaatgcatt ccaatactgt atattcattc
    24001 aggtcaattt gtgtcataat taaccgtttg tgatcgccgg tagcaccatg ccaccgggca
    24061 aaaaagcgtt taatccggga aaggtgaatg gataagatcg aagttcgggg cgcccgcacc
    24121 cataatctca aaaacatcaa cctcgttatc ccccgcgaca agcttattgt cgtgaccggg
    24181 ctttcgggtt ctggcaaatc ctcgctcgct ttcgacacct tatatgccga agggcagcgc
    24241 cgttacgttg aatccctttc cgcctacgcg cggcagtttc tgtcactgat ggagaagccg
    24301 gacgtcgacc atattgaagg gctttctcct gccatctcaa ttgagcagaa atcgacgtct
    24361 cataacccgc gctctacggt ggggacaatc accgaaatcc acgactattt gcgtctgctg
    24421 tacgctcgcg ttggtgagcc gcgctgcccg gaccacgatg taccgctggc ggcgcaaacc
    24481 gtcagtcaga tggtggataa cgtgctttcg cagccggaag gcaagcgtct gatgctgctc
    24541 gcgccaatca ttaaagagcg caaaggcgaa cacaccaaaa cgctggaaaa tctggcaagc
    24601 cagggttaca tccgtgctcg tattgatggc gaagtctgcg atctttccga tccgccgaaa
    24661 ctggaactgc aaaagaaaca taccattgaa gtggtggttg atcgcttcaa agtgcgtgac
    24721 gatcttaccc aacgtcttgc cgagtcgttt gaaaccgcgc tggagctttc cggtggtacc
    24781 gcggtagtgg cggatatgga cgacccgaaa gcggaagagc tgctgttctc cgccaacttt
    24841 gcctgcccta tttgcggtta cagtatgcgc gagctggaac ctcgcctgtt ttcatttaac
    24901 aacccagcgg gagcctgccc gacctgtgac ggtcttggcg tacagcaata tttcgatcct
    24961 gaccgcgtga tccagaatcc ggaattgtcg ctggctggcg gtgcgatccg tggctgggat
    25021 cgccgcaact tctattattt ccagatgctg aaatcgctgg cagatcacta taagttcgac
    25081 gtcgaagcgc cgtggggcag cctgagcgcg aacgtgcata aagtggtgtt gtacggttct
    25141 ggcaaagaaa atattgaatt caaatacata aacgatcgtg gcgatacctc cattcgtcgt
    25201 catccgttcg aaggcgtgct gcacaatatg gagcgccgtt ataaagagac ggaatccagt
    25261 gcggtacgtg aagaattagc caagtttatc agcaatcgtc cgtgcgccag ctgcgaagga
    25321 acccgtctgc gtcgggaagc acgccacgtg tatgtcgaga atacgccgct gcccgccatc
    25381 tccgacatga gcatcggtca tgcgatggaa ttcttcaaca atctcaaact cgcaggtcag
    25441 cgggcgaaga ttgcagaaaa aatccttaaa gagatcggcg atcgtctgaa attcctcgtt
    25501 aacgtcggcc tgaattacct gacactttcc cgctcggcag agacactttc cggcggtgaa
    25561 gcccagcgta ttcgtctggc aagccagatt ggtgcaggcc tggttggcgt gatgtacgtg
    25621 ctggatgagc cgtctatcgg cctgcaccag cgcgataacg agcgcctgct gggtacgctt
    25681 atccatctgc gcgatctcgg taataccgtg attgtggtgg agcacgacga agacgcgatt
    25741 cgcgccgctg atcatgtgat cgatatcggt ccgggtgcgg gtgtacacgg cggtgaagtc
    25801 gtcgcggaag gtccgctgga agcaataatg gcggtgcctg agtcgttgac cgggcagtac
    25861 atgagcggta aacgcaagat tgaagtgccg aagaaacgcg ttccggcaaa tccggaaaaa
    25921 gtgctgaaac tgacgggcgc acgcggcaac aacctgaaag atgtgacgct aacgctgcca
    25981 gttggtctgt ttacctgtat tactggggtt tcaggttccg gtaaatcgac gctgattaac
    26041 gacacactgt tcccgattgc ccaacgccag ttgaatggtg cgaccatcgc cgaaccggca
    26101 ccgtatcgcg atattcaggg actggagcat ttcgataaag tgatcgatat cgaccaaagc
    26161 ccaattggtc gtactccgcg ttctaacccg gcgacctata ccggcgtgtt tacacctgtg
    26221 cgcgaactgt ttgcgggcgt accggaatcc cgtgcgcgcg gctatacgcc aggacgtttc
    26281 agctttaacg tccgtggcgg acgctgcgag gcctgtcagg gcgacggcgt gatcaaagtg
    26341 gagatgcact tcctgccgga catttacgta ccgtgcgacc agtgcaaagg caaacgctat
    26401 aaccgtgaaa cgctggagat taagtacaaa ggcaaaacca tccacgaagt gctggatatg
    26461 accatcgaag aggcgcgtga gttctttgat gcggtgccag ctctggcgcg taagctgcaa
    26521 acgttgatgg acgttggcct gacgtacatt cgcctcgggc agtccgcaac cacactttct
    26581 ggtggtgaag cccagcgcgt gaagctggcg cgtgagctgt caaaacgcgg caccgggcag
    26641 acgctgtata ttcttgacga gccgaccacc ggtctgcact tcgccgatat tcagcaactg
    26701 ctcgacgtac tgcataaact gcgcgatcag ggcaatacca ttgtggtaat tgagcacaat
    26761 ctcgacgtga tcaaaaccgc tgactggatt gtcgacctgg gaccggaagg cggcagtggg
    26821 ggcggcgaaa tcctcgtctc cggtacgcca gaaaccgtcg cagagtgcga agcttcgcat
    26881 acggcgcgct tcctcaagcc gatgctgtaa tcgttaaggc cgctttcaga gcggccttct
    26941 tctttcagag ttgcaccagc aatttacgtt tttcttccgg cagcaaattc accgcctgct
    27001 gataagacgc atccaccaga taatagattt gcgaatccgg cagcgaaccg tcgagataca
    27061 cggtgctcca gtgcgcttta ttcagatggc ggcttggacg cacatcgctg tgctgctgac
    27121 gtagcagctc cgccagctcc gggctggttt tcagcgaaac agctgggcga ttttctactt
    27181 ctttcaccat cgcaaacagc acatcttcca ctttgatctg cgtcgctttc cagtcgttat
    27241 gcacgctctg tcctgcgcct ggttttgcca tgcaatattg tagcaactcc gaaatggtca
    27301 tttttcactc cccttgtagt gtcgcgatga tgcgacgcga tccgccgtgg atgcgatgtt
    27361 cccccagcca gatgccttgc caggtgccgg tctgaatacg ccctttatgc accggcaata
    27421 caagcgatgt tcccagcatt gaggatttga tatgagaagg catatcgtct gctccctcat
    27481 agtcatgctc ataatttccg ttatcgggaa cggcgcggag gaaaaaacgc tccatgtcgt
    27541 ggcgtacggt gggatcgcag ttctcattaa gtgtcagaga ggcggaggta tgttgcagca
    27601 acagatgcag taagccgatg ttaacgcgcg gcatatcagc cagctgattc agaatttcat
    27661 ccgttaccag atgaaaccca cgagatttgg cgctaagcgt gagcgtcttt tgataccaca
    27721 tgtgctgctc cttgataaaa ctctcttaat cagtttgcag caagacagcg aaaggataaa
    27781 ggtgtgatta aaaaaacagc attcgggaga gtattacgct ctcccgctct gtcagtattc
    27841 tgaattgacg atcacctctt caccaaacgc acccgcttgt ggcaagggtt tgtaggtaga
    27901 gttggaggcg cgcagaatgc ggataccacg agcgccgaca tcgcgtgcgg cggtaatatc
    27961 gttatcggaa tcaccataaa aaattcggat atttttatcc tgcagccatt gcgattttgt
    28021 attttgccct ggtttatcgc ccgcaaagat caccggattc atgttggtgg caggaatatg
    28081 aaaattatcc gccagcgttt ttgaaaccgt ttctgttttg gtcgggctac gaccagtcac
    28141 aaagaagatc gcgtcaccgc ggcgtacatg catatcaatc agctggcgag cgacctcttt
    28201 tggaatgctg aattcatccc agccattgtt cattttttcc cagaacacag gatttttcag
    28261 ataatcttcg ctttctggcg agaaggtttt tttgccgcgc cagaagcccg gactggaaaa
    28321 aagtaccgtg tcatcgatat caaaccccac cgccattggc ggacgtcctg cgaggctatt
    28381 ttcaatttgt gcaaccgaaa cccaatgaat tggcgcctgt tcagcgagtt tggctacgtt
    28441 agtacccgga ttaagcggtg aaggagatga ggccagggca acagcggaac tgtttagagc
    28501 gaacaataag caaacggcac tcaatgcctg tgtgatcttg cgcatatttt tccctaaata
    28561 gtcagtaagt tgaaacttat tattgtgcgt ttgttgaaaa aaccatctga ccataacgcc
    28621 aaccgcgggg gaaaggaagg attatctgcg gatttgtcac ataaaaagag catgatcaca
    28681 tttggaatac ctgtgcgata aaccacacat tgccacggtt attctgctta cacattcaca
    28741 caatggcact cttttggttc tcgcaaacat tgttcaggga atatactgat gataaatata
    28801 aatgcattca atggatgatg aatgaaaata tcgtggaatt atatatttaa gaacaaatgg
    28861 cgatttcaca ttacaagcat ttcacttttt cttatcatgc tcgcggtttc aatcgctttt
    28921 ttgcacttgc gttttaatac cttttccggt accgataaaa tgcggcttga aatgtataag
    28981 tccacattat attccaccat cgagcaattt tatgttttac cctatatgct ctcaacagac
    29041 catatcatcc gtcaggcggt aattacgcct gacgatatga cgtccagcga actcaatcaa
    29101 cgaattgcac atttcaatac tcaactcaaa accgcagcaa tatttattct ggatacccaa
    29161 ggtaaggcca tcgcttctag caactggcag gaccccggca gctatgtagg gcaaaattat
    29221 agctatcgcc cctattataa acacgccatg tctggcttaa atggacgctt ttacggtatt
    29281 ggtagcacta cgaatacacc gggattcttc ctctctacaa gtataaaaga taaaggaaaa
    29341 attgtcggtg ttgtagtagt aaaaataagt cttaatgaaa ttgaaaaagc atgggccgaa
    29401 ggtcctgaaa atattatcgt gaatgatgaa catgggatta tatttttaag ttcaaaatcg
    29461 ccatggcgaa tgcgaacact gcaaccgtta cctgttcagg caaaacaaaa actacaatct
    29521 acccgccaat atagtctcga caatctttta ccggcggatt attatccctg ttataccgtg
    29581 agcaatttta ctttcctgaa agataaaaaa gaacaactct gtttattccc gcaatattat
    29641 acgcaacaaa tagccattcc agaatttaac tggaaaatga caattatggt ccccttagat
    29701 aacctgtact ggtcatgggc tattttgtta gtcattacac taattattta cctgctgttt
    29761 ttgttattta ttaaatactg gagaatgcga tctcatgcac aacaattatt aacactggcg
    29821 aatgaaacat tagaaaaaca ggttaaagag cgtacatctg ccctggaatt gatcaatcaa
    29881 aaattaatac aggagataaa agagcgcagt caggctgaac aagtattaca aattacgcgt
    29941 agtgaactgg cagagtccag caaactggcg gcgcttggac agatggcaac cgaaattgcc
    30001 catgaacaaa atcaaccgtt agccgccatt cacgcactta ctgataacgc gcgtactatg
    30061 ctaaaaaaag agatgtatcc gcaggttgaa cagaatctga aacacattat ttcagtgatt
    30121 gagcggatga cgcagctcat ttccgaactt aaagcatttg cctcgcgcca tcgcgtacct
    30181 aaaggttctg ccgatgtcat caaagtgatg tatagcgccg tggcgttact taatcacagc
    30241 atggagaaaa ataacattga gcgacgaata aaagccccat ccatgccgtt atttgtcaat
    30301 tgcgatgagc tcggtcttga acagatattc agtaatttaa ttagcaacgc cttagattct
    30361 atggaaggta gctcttacaa acgactggat atcgccattc gccaggcaaa taacaaagtt
    30421 attattacca ttaaagacag cggtggcggt tttgcacctg aagttgtcga tcgcatattt
    30481 gaaccatttt ttaccactaa acgtagagga atggggttgg gactggcaat agtcagcgaa
    30541 attgtccgaa attcgaacgg cgcactccac gccagtaatc atcctgaagg cggcgcagta
    30601 atgacattaa cctggcctga atggggagaa gaacatgaat aataaatcca gtgaaattac
    30661 tatagtttat attgaggata gtgacgatgt acgttttgcc tgtgaacaaa cgctcacgct
    30721 ggctggttat cgtgttattt cctgttgcga tgcagaacac agtattcccc ttattcaaag
    30781 tcaggccaac attattatac tgaccgatgt caggttacct ggaatatcag gactagagct
    30841 cctcagttac atcaacgaaa tggatagcaa aattccggtt attttaatta ccggacacgg
    30901 tgacgtggaa atggcggtgg atgccatgcg taatggtgca ttcgatttca ttgagaaacc
    30961 aagtagctca gataaattat tgagtattat tgccagagcg gtagaaaaac ggcgattggt
    31021 tttagaaaat cagcaattac tggcgaatct gcaacaggaa aatggcccgg tgctgattgg
    31081 tcgtagtccg cagatgcagc aactgcgtaa aatgatcctt aatgtcgcag ataccggtgc
    31141 cgatgtcctt atttatggtg aaacaggttg tggtaaagaa gtcgtcgcca gaatgctgca
    31201 tcactggagt acccgccgtc aggggcagtt tgttgcctta aactgtgcag gcttaccaga
    31261 aacgttgttt gaaagcgaaa tatttggtca tgaggcgggc gcatttaccg gtgcggttaa
    31321 aaagcgtatt ggaaaaattg aacatgcaaa tggtggtacg ttatttctcg atgaaataga
    31381 gggaatgcca tcaggaatgc aggtaaaact gctacgtgta ttgcaggaaa gaaccattga
    31441 acgattaggt gccaatcaat taattccggt gaattgccgt gttattgcag cgaccaagga
    31501 agatttactg cgtcgcagcg aagaacatct gtttcgtctc gatctctatt atcgtctgaa
    31561 tgtggttagc ctgaatattc cacctctacg ccaacgacga gaagatatcc ctgaactgtt
    31621 ttactggttc gccagtcagg cggcacaaaa atataatcgt cctctgcctg atatttctcc
    31681 catgttactg gcctggttgc agtcacaaag ttggcctggc aatgtacgtg aacttaaaca
    31741 caatgcagag cgttttgttc tcggtttact gacccaccac cagccagtac caatgactca
    31801 gcaagaagag agtggcctta ccgcctgcat tgatgctttc gagaaaaagt taatcgaaga
    31861 tatgctgcga caaacggaag gccaggtcag tttgacagcg cggctattac agctgccgcg
    31921 caagacttta tatgacaaac tcaacaaaca tcagattcaa ccgcaagttt atcgaccaga
    31981 atcatccagc taatgctgat tcctgaggat gtaaccccaa atcaatacct ttgcgggcgt
    32041 aatgttcaac tacccaggac cagaatgata ccgggatcgt aatcatcttc gcattgcgtt
    32101 cccggttggc agcttctggc ataccggggt aaatcaccgg attgccgcca ggttgaggct
    32161 gcgtagctaa cagataatcc gcaaagaact gccctttctc ccctgccaga tcggtgaatt
    32221 gatcaggatc gataatcaac gcaaaaagat gattatgcgc agatccatcc gtctgatgat
    32281 cttcggcgat cgtgtcacca ccagataaga tccccgccat catttcaatc atcagtgcca
    32341 ggccaaatcc tttatgctca ccaaatggca gtaacgcccc tggaggattg ctgaataaca
    32401 ccccaggatc attgcttgga ttccccagtg catcaatcaa ttgtcccggc gcggcctgct
    32461 tcccggtgga cgacattacc cgtgctttat ttagcgccat gctactggtg gcaaaatcaa
    32521 ccagaatcgg cggacgccca tcactgaacg gccaggcaaa gcagaaaggg ttagtggtca
    32581 tacgcggttc cgcaccgccc cacggggcga ccagtgcagg acgcccggtg acgttactca
    32641 tggcaagcat cgccaggcct tgatcagcaa ccatttgccc gtaatgcccc atacgcccta
    32701 aatgatgcgc atggcacaac gttaacaggc aaaaaccgcg gtgttttgct tgttcgatgg
    32761 cagcatttac cgccactttt ccggcgtgtt gaccaaagcc attgtgggca tgaaaacgtt
    32821 gtaaattacc ttcacttgtt aagcattccg ggcgggcatt cgcggttaca tcgccacgag
    32881 cgatattttc cagatatttc ggtagcagcg tgacgccgtg gctggcaaag ccataacgtt
    32941 ccgcttcaat catatgttcc gccacatcct gagcgatatc ggcatcacag ccattaagtt
    33001 ccagcagccc ggcagctaac caacgcactt tttcaacagc tatttgtaat gacatcgttg
    33061 gtttcctcac attagaccta aaattttcca ccaggtggcg gcaaacagta gcaacaacag
    33121 gtagccgatc accgtcaggt acagtcccac acgggtaaac tggcgtggcg tgaaggtatc
    33181 cgtacccata cacaccatat tttgtggcgc gttaatcggc aggatgaagc caaaactgac
    33241 actgaaggcg agcagaatgg tcatgccgac cgggttaacg ccgagttctg gcggcagact
    33301 gcttaacagg ctaatcagga tgggcagcaa tgcggcagtt agcgcagtgg cactggcaaa
    33361 ccccaggtga atgattatca agaaagcggc gagaatcgcg aagatggcta atgatggcaa
    33421 gccatcgaga ccaaaacctt tcaccacata gttcgccatc catgaagctg cctgcgtatc
    33481 aagcagcgtg gaccccaggc tgataccaat accaaacata agcaacgtgc cccactgcac
    33541 acgtttttcg acctctttcc agctcattac accgatgcct ggcagcaaca taatcgccag
    33601 tccggcaagg gtgacagagg tggtatcaat actgtgtaat tttccgccag tagaccaaaa
    33661 gagcagtaac aatagcgaaa taccgatcaa acgtttttct ttaccagtgg ttggccctaa
    33721 ctctgcaagt gcttttttaa tggcctcgct ccccccttca acagcttccg tttctggcgg
    33781 caacaattta cgtgcgagga aatagaggat cgccgacatg gtgaggctcc agggcgcgcc
    33841 cgctaacagc cagtcaagcc agctgacaga atgcccggcg ccgaaagttt tattgataaa
    33901 accgatagaa agcaggtttt gcgccgccga agtttgaata ccaacgttcc agatactggt
    33961 cgcctgggcg ataacaatca tcattgacgc cgctaaacgt gaatgtttat ccaccttaaa
    34021 tgccgcaatg acccccatca taatcggcac cacgcaagcc gtacgggcag tggcactggg
    34081 tacaaccagg ctcaggacga tggtcaccac aatggcaccg ataataatgc tgcggctgct
    34141 ggcacctatt ttcgacatag taaacagcgc aatgcgttta tccagctcgg taatggtcat
    34201 tgccgccgcg ataaacattg ctgctgccac cagcgccaga gcagaattag aaaaacccga
    34261 cagagtcatt ttcagcgctt tcgcggtgcc aaggatcgtg tccgggtgat tcatatccgg
    34321 ggcaaaacca accataaaga taatcagtgc ggaaataaca attgcactgg cggtgtaatc
    34381 catcgcttcg ctgatccaga caataatggc aaacaccaga atggcgatca tattttgtcc
    34441 agcgacgggc aaggtatcgc ccagtggcag aagggtaata ataattccgg caatgatcgc
    34501 caaccacagg ccgggattag ggataattcg acgcttttgt ggtgcgccat gggatattga
    34561 tgacatattc acctcattgt gaaaaataaa aaacctgcct ggctattgca ggagctatgc
    34621 catctttaat tttttaataa cgctttgtta tttaatgagt tagctattta tcctttttat
    34681 tttatgcgcg gttttccgca cagttattaa aagatgattc ccttaccaca cagataaaaa
    34741 aagggagcga tatgctccct tgatattcat ttatggatta cttaataaca ttccataacg
    34801 tttcgccaag caacgccggg tttcttgtcc atgccatgcc tgctttatcg aacgcacgga
    34861 atttatcctc tgcactgccg ctaccgccag aaataatggc tccggcgtgc cccatgcgtt
    34921 ttccttttgg tgcagtaaca ccggcaatat aacctacgac cggtttattc atttcatgac
    34981 gaataaattc cgcagcccgt tcttccgcat taccaccaat ttcaccgatc ataattaccg
    35041 cgtctgtttg cggatcattt tcgaataaac gcagcgcatc aataaagtta gtccccggaa
    35101 ccggatcgcc gccaatacca atacaggtgg attgcccaag tcccagcgcc gtggtttgcg
    35161 ctacagcttc gtaggttaat gtaccggagc gggaaacaat cccaatacgc cctgggcggt
    35221 ggatttcacc aggcataatc ccgattttgc actcgcccgg cgtgataact cctgggcagt
    35281 ttggcccaat cagccgcaca tccgggtggc agtcaagcag ccgacgaact ttcaccatat
    35341 ccagtgtcgg tacaccttca gtgatcacca caaccaattt gacgccagct tcaatagcct
    35401 caacaacaga atccagcaca aacggtgcag gcacataaat cacgctggca tcagcctcag
    35461 tttgtcgcat cgcttctttc attgaatcaa aaaccgggcg atccagatgc cgttgcccgc
    35521 ccttacctgg cgtcactcca cccacaatct gcgtgccata cgcaatagcc tgctctgtat
    35581 gaaatgtgcc gtttttgccg gtaatgccct gcaccagtac ccgcgtgtgt ttgttgatca
    35641 aaacgctcat cttaacctcc tgttaattca gtaacgcaat gatgcgttta gcggcatcat
    35701 ccagagaatt gacggcttcc accgtcaggc cactttctgc cagtaaccgc tgcccttcgg
    35761 cagcgttatt accggagaga cgcaccacga caggcagggt gatacgggcc tcattcagag
    35821 catgaataat ggctctggcg atcatgtcgc aacggacaat cccaccaaaa atattaacca
    35881 gaatggcttt cactttgctg tcggaaacaa tcagccgaaa ggcttcactg acgcgctcct
    35941 gggttgcgcc accgccaaca tctaggaagt ttgcgggttg ttcaccgtat agcttgatga
    36001 tgtccatggt tgccatagcc agccctgcac cattgaccat gcagccgata tttccgtcca
    36061 gcgagacata attaagatca agcttcgctg cctggctttc gcgtggatct tcctgggttt
    36121 catcacgtag cacctggagt tccgggtgac gatacagcgc gttatcatcc agcgatactt
    36181 tcgcgtctgc acacataaat tcgcccgttt cccgcaacac cagagggtta atttccagca
    36241 gggcgaaatc cagttcgtta aaggctttcc acgcctgatt aaccaggcga ctaaaggtag
    36301 caaatagccc gtgttccagt tgcagaacag caaacatttc gcggatatgg caaggctgca
    36361 cgcctgtcag tggatcaatg ctgacactgc tgattttctc cggtgtctca tgggcgactt
    36421 tttcaatttc cacaccacct tccgggctga caataaacgt gacccgctgg ctttcacgat
    36481 ccaccaccat gccaaaatag agttcctggc gtaccggata gatgttttcg cacagcaaaa
    36541 tactgctgac atactgccct tctggcccgg tctgataagt caccagttgc gatcccaaca
    36601 tttgctgtac aaaagcctgt gcttccggca gctgcttaag cacttttacg ccgccagctt
    36661 taccgcgccc accagcatgt acctgcgctt taagtaccgc gcctttactc gggcaagcaa
    36721 tatgttgcca ggcatctgcc agttgcgaaa tctgttggat ggctatctct tttggacacg
    36781 gcatccccat gccagccagt aacgatttgg cctgatactc atgtaaattc atatattccc
    36841 tcagtggtat gtgttatccg tgtaatgctt tattattcaa ggccattgct gcttcgtgga
    36901 tgacttcgct taatgtcgga tgcgcatgga tcgcacaggc aatatcctcg ccagaggcgc
    36961 taaaagtcat cgccagcgca atttcattga tcagctccga tgcctgcggg ccaacaatcg
    37021 ctccgccaag tacgcggtca gtgtgtttat cgctgtataa cgtgcaacgc ccaccttcct
    37081 ggcctaatgc taaggcgcga ccgttaccgg caaacagaga atttcctttg ttaaagactc
    37141 tgccagcggc tttcagtgag gcctcgtttt cccctaccca cgcaacttcc ggttgggtgt
    37201 aaatcaccga agggatcagc gcaaaattaa tcggctctac cgccagtccg gcaatttgat
    37261 cagcaaccac cacaccttca gccatcgctt tatgcgccag catcggccct cgcacgacat
    37321 caccaattgc ccacagcccg gctttcccgg tacggcataa gttatcgacg gcaataccgc
    37381 cacggttatc ggcttcaaga ccgagttgca cgagatcgac accagaaagt cgcggtacac
    37441 gtccaatggc aagaattaat ttatcgaagc ggctttcttc tcgcttctca ccctgccgcc
    37501 agcgcacatg cacaccgtca tcccgttgtt caatagcctc aatctctact gccagttgca
    37561 ttttcatccc actggcaatc attgctttac ggacttcgtt cgacaggcga gcttccagtg
    37621 caggcaagaa cgttggcgcc atctccagca acgtgacgtc tgaaccgacc cgattccaca
    37681 cagaccccag ttcaaggcca atcacgccag cgccgatcac accgagacgc ggcggcacag
    37741 cggttaacgc cagggcgccg cgattatcga gaatttgctg attatcaatg gtaacgccag
    37801 gcagttgacg cggctgagaa cctgttgcaa tcaccacatt acgggcgcgg atttgttgat
    37861 cgtttacacg taattgccaa atctcatccc gagcatgttc taacgttgcg aggccgcaca
    37921 ggtacttcac tttatttttc ttaaacagca agccgatgcc cattgtcagg cggcttacta
    37981 tggcgtcttt acgttgaatc atcgcagctg cattgaaaga tacgccgtca acattaacgc
    38041 catgaatgct cgcctcatgc tgcacttgcg cgtacagttc ggaagattgc agcaacgatt
    38101 ttgacggaat acagccgaca ttgagacacg taccgcccgg cgaaggttcg ccctgtgcat
    38161 taacaccatc atcaatacat actacggaaa gcccattctg cgcagcccgc agtgccgcta
    38221 cgtaaccacc tggcccaccc cccataactg ccacatcaaa aattgtactc atcggcgccc
    38281 tccttaaaga tccagcaaca gttgttccgg tgattccagt agctcacgta ttgccaccaa
    38341 tgtctgcacg gcttcctgac catcgataat gcgatggtca tagctgagag caaggtacat
    38401 catgggacga ataacaacct ggccgttttc tgcgaccggg cgcggcgtga tggcatgcat
    38461 cccaagaatg gccgactgcg gcgggttaat gattggcgtt gacatcatcg aaccaaaagt
    38521 accgccgttg gtgatagaga acgtgccgcc ctgtaacgcc tccagcggca atttgccatt
    38581 gcgtgcctgt gtggcgtatt cggcaatttg ccgttcaatt tcccccaaag agagtgattg
    38641 cgcattgcgt aacacaggca ctaccaggcc gcggttgctg ctcactgcaa tgccgatatc
    38701 gcaataatca cgccagataa tctcgttgcc atcaacgctg gcattcacta cagggaagcg
    38761 ctccagcgcc cgggtaacgg ccttaacaaa gaaggacata aagcccagct tcacgccgtg
    38821 cttctcggca aaacgatctt tccagcgggt gcgtaaatcc atcacgctct gcatgtttac
    38881 ttcgttgaat gtggtaagaa tggcgttatt ttgttgggaa gccagcagac gctcagcaat
    38941 tcgctgacgt aagcgcgaca tcggctcacg acgttcatga cgagcacccg gtgtcaaggg
    39001 tttagctggc gcgatctccg ccacccgttc aggctgagtg gctggtgcag gagtaacgcg
    39061 ctgcacatcc tctttcagga tacgtccatt tctcccgctc cccgcaacgt cagcaagttc
    39121 gacaccacta cgctgtgcct ccagccgtgc ggaaggcatc gccagggttt cggtgactgg
    39181 tgtgacggtt tcttctatga cagcttgcgg cttaagatgc gccaacagtt gcgcggacgt
    39241 gaccgtgcta ccttcgctga cgataatatt gcttaacacg ccatcgtgcg gggccggtat
    39301 ttccagaatg actttatcgg tttccagctc ggcaatcaca tcatcgcgtt ttacgtgctc
    39361 gccttcttgt ttacaccagg ttgtcagcgt gccttccgtg acagattccg gtaatacagg
    39421 gacagtaatt tcgatcataa aatgttctcc ttattatttt tgtgcgcctt tcatggctga
    39481 atatctgcaa acgcagcagc aagaaattcg tcgatttgtt gtttatgtac acgcccatac
    39541 cctgtcgccg gagctgccgc tgccgggcgg cctgcatact gccagtaagg agtgttaatt
    39601 ttcagtgccg ccagctcatg acgaatctgc cgccacgcgc cctggttttc tggctcttcc
    39661 tgcaaccaaa tccattcgca gcagttcggc caactcgcca gaacatcatt cagttctgcg
    39721 accgggaacg ggtagagttg ctccaggcga acaatcgcca cttcgtcttc atgctcacgc
    39781 tgcttacggg cttccagtac gtcgtaatag acctggccgc tacacaaaat aacgcgctta
    39841 actttctggg actgatgcaa ttgcggatcg gtgattaccg gtttataagc gccatcagtg
    39901 aattcacaaa gctcgctcat agcgccttta aaacgaagta aacgcttact catcattatc
    39961 actaacggtt tgcgcattgg gcgtaatgcc tgaccgcgca acaaatggaa catttgtgct
    40021 gactcactgg gcatcaccac ctgcatgttg ttttctgcgc acagttgcag ccagcgctca
    40081 ggacgcgcag aagagtgctc cggcccttgt ccgtcatagc catgcggcag aagaatggtt
    40141 aaaccgctat agcgatccca cttggtttcg ccagacgaga taaactggtc aatagcaacc
    40201 tgagcgccgt tggcaaagtc accaaactgt gcttcccaaa taaccagttg ctgaggcgca
    40261 gacgttgaat agccatattc aaaggctaag agcgcttctt cattaagcac cgaatcataa
    40321 acatcgaaag atgcctgacc agcgcggata tgctgtaacg gcagataacg acgggcttca
    40381 gtttgatgat gtaccactgc atggcgatga ctgaacgtgc cacgccctga gtcttcacca
    40441 gaaagacgca caccaacacc cgcatccaca agcgatgcat aggccagcat ttcagccatt
    40501 ccccagtcta ctggctgagt acctgcggcc atatcctggc gtaacgccag ttgccgttta
    40561 atggtaggat gtgcgaccac atcgggtggc agtgtagaaa tgatttcacc atacgctacc
    40621 agcttttgcc gaggcaatgc cgtagatacc ggggcgctcc agtgagggtt ggtcaaccca
    40681 taccagttag cgctaaaaga gtgaattgca ggtttgagag gctgcggttc acgtttctgg
    40741 cagctatcca gccagtcgcg atagcgtgcc gtcatctcat cttgttgcgc ctgagttaac
    40801 aggccgcggc gggcaagaga ttcaccataa cgggcgagcg taccagggtg agcatccact
    40861 gcctgataca tttgtggctg ggtaagacgt ggctcatcgc tttcgttgtg tccgtgctta
    40921 cggaagcagc aaatatcgat gatgatgtcg cgccggaagg tgtctcgcca ttcgcacgcc
    40981 agttccatta cctggcaaac agcgtcaacg tcatcgccat taacgtggat aattggggcc
    41041 gctaccattt tggcaatatc ggtgcagtac cgtgaagaac gcatatcctg caagcgggaa
    41101 gtggtgaagc caatctgatt gttaatcacc agatgtagcg taccgccagt gccgtagccc
    41161 tgagtctgcg ataagttaaa cgtggtttgg ttgacgccga ggccgcccaa cgcggaatcc
    41221 ccatgaataa gtacgcccac cacttttgcc tgaccatcct caccacggcg ttcctgcctg
    41281 gctcgcacct ggcccagcac caccggattc acgatctcca gatgtgaggg gttatacgcc
    41341 agcgccacat gcagtgaacc ggcaggtgtt tccagattgc tggaataccc catgtggtat
    41401 ttcacatcgc ctgaaccact gccgatcgtc tgcttgcctt caaactcagc aaaaagctgt
    41461 gccgggtctt tattcaacag attaaccaga acattaagtc ttccccggtg cgccatgcca
    41521 atgaccattt cctccacacc ttgcgcacgc aggcgttttg tcagcgtatc gagcgccggg
    41581 attgcacttt cgcccccttc cagagaaaac cgtttctgcc caacataacg ggtatgcaga
    41641 taacgctcta acgtctctgc cgccatcagt ttttcaaaac gggcaatgca ggtttgggca
    41701 tcagcttgtg gcgcattgct gctctcaatc cgtgataaca accagttgat ttcttcacga
    41761 ttttccagat gagcaagttc ataagcctgg ctaccggccc aggcttgctc tagcaggtta
    41821 agcagttgct taagtggcat ttgcgtggtg tgtgcgccaa aggtgacgct aaactcctgc
    41881 aacaagtctt cttcgcttaa tccccaaaaa ccaggctgga gggagggaac gtcagctggg
    41941 ggattcaaac caagtggatc aagctttgcg cgcagatgcc cctgcgtgcg ccaggcattg
    42001 atcagttgta ttaccgcagc ctgttttttt aacgtcgcac cgctgacgtt gttatcaccg
    42061 gatacgcttt ggacagtcag ttcaggtgaa agaaaaaaac gccgccagtc ttccgggagt
    42121 tgttccggcg tacgcaacca ggtctggtag tactcttcaa gccaggtcgc gttatctcca
    42181 ctaagcagcg tgccgggcga ggtcatgttc tccatgtttt tctcctgatg gtcatagaca
    42241 gccgcattac tgcacaatcc gtaccaacag ttaaaaacac taattttcaa aaacttatag
    42301 aaaagagatg atgtaaaagt gggcgaaaaa agggacagaa tagtagggga tggctggagt
    42361 caggaacagg cggagagatc cgcctgttga ttaaaatcac atcaccgcag caaacgcctt
    42421 tgccacgcga tgcacatttt gcgcgttaag cccggcgaca cacatgcgac cgctggcgat
    42481 gagatagaca ccaaattctt cacgtagtcg gtcaacctga gcggttctta aaccggtata
    42541 actgaacatg ccgcgctgat taagcagata atcgaaattg cgctctggca tctctgtgct
    42601 caataccttc accagttcct gacgcattgc cagaatgcga gttcgcatct cttctacttc
    42661 cgccagccag ctggctttca atgcctcgtc attcagcacc gcagccacca cctgcgcacc
    42721 aaaattcggc gggctggagt agttgcggcg aacggttgct ttcaattgcc ccagtacgcg
    42781 gcctgcggct tcggcatctt cacacagaac agaaagtccg ccgacgcgct cgccgtaaag
    42841 ggagaaaatt ttcgagaacg aattgctcac cagagcgggt aatccagcgc tggcaatggc
    42901 gcgaatggcg taggcatcct cttccatacc ggcaccaaat ccttgatagg caatatcgag
    42961 gaatggaata agctcacggg ctttgagaat ttcaatcacg gaatcccact ggtcattagt
    43021 gagatcggca cccgttgggt tgtggcaaca tggatgcagc aacacaatac tgcgggcagg
    43081 taatgttttc agcgtcgcca acaggtcatt aaagcgcacg ccgttagtcg cttcgtcata
    43141 ccaggggtaa gtgctcactt cgaatccagc cccggcgaat attgctacgt ggttttccca
    43201 ggtaggatcg ctgacccaga cgcctgattc cgggaagtag cgtttcagga aatctgcacc
    43261 cactttcaat gcccctgagc cgccaagggt ttgaatggtt gctacgcgct gttgttgcag
    43321 taccggatgg tccgcaccaa acagcagcgg cgcaatggca tggcgatagc tgttaagccc
    43381 ttccatcggt aaataaagcg aagcaccatg aggctgcgca ttcaggcgcg cttccgcatc
    43441 cgccacggct ttcagttgtg gaataattcc gtcttcgttg tagtacagac cgatacttaa
    43501 attcactttg tcgctgcgag ggtcttcttt aaaacgctcc ataagcgtaa gaatcgggtc
    43561 gccagcgtag gcgtcaactt tttgaaacac gcgatggttc tccaggttta cgggcaggtg
    43621 gttaaaacac aataaaccgg aagaaggcga agatcgagag gatgttcagg ggcgaatggc
    43681 aattagcaac agagtgaggc tcatgacaaa cgtacatccg ccagatgcac gacctttata
    43741 agagcatgaa agaatatcaa cttattgaat tggtacgatt ttattggccg gataaggcat
    43801 tcacgccgca tccggcacag acaatcaaat attaaagaac gattaatcca cgtatttcat
    43861 cgcgactctt gaagtcaggc gcgtaataag ttcgtaagcg cttactttcg tcatttcagc
    43921 gatacgttct acgggcaaac cttcgcccca taaaatgacc gggtccccgg ctttgtcctg
    43981 cgcctgtgga cctaagtcta cgcagatcat atccatcgcc acgcgcccga caatcggcac
    44041 ttcgcgaccg ttcaccagca ctggcgtacc ggacggcgcg gcgcgcggat aaccatcgcc
    44101 atagcccatc gcgactacgc caagacgagt atcacgttcg cttacccagg ttccaccata
    44161 accgacaggc tctccggctt tatgctcacg cacggcaatc aggctggagg ttagcgacat
    44221 cactggctga cagccaaaat cggcaccggt ggagcgatct tccagcggag atacgccgta
    44281 aagaatgatg cccgggcgca cccagtcaaa atgcgactgt ggccagagta aaatgccacc
    44341 cgacgcggca atggaacgtt gaccaggttt gccttcgcaa aaggtattaa agatagcgag
    44401 ttgcttctcg gttgcgccgc attttggttc atccgcgcgc gcaaaatggc tgacgatatt
    44461 caccggctga cgaacgtttt tgcactgggt cagacgatga taaaacgcct cagcctgttc
    44521 cggccttacg cccagacggt gcataccggt atcgagtttc atccagacgg taaccggctc
    44581 gtccaggcta gcctcttcca gcgcagccag ctgttcttcg ttatgcacgg cggtatgaaa
    44641 atgttgcgca gaaatcgtcg gtagatctct ggcatcaaaa aagccttcga gtaaaagtac
    44701 aggtttggtg attccccccg cacgcagtcg cagagcttct tcgagacggg ctacgccaaa
    44761 ggcgtcagca tcggggagcg ttcgcgcggt ctcaagaaga ccgtgaccat aagcgttcgc
    44821 tttcaccacc gcaaccattt tactggcagg cgccagttca cgaagacgtt gcaggttgtg
    44881 tcgcagagcg cggcggttaa tcacaacagt tgccgcttgc atttgtgttc cttgataagt
    44941 gtttgcttta attacctaat tcataaaata attattattc gtcgtcgtac tgcggccccg
    45001 catagttgtc gaagcgcgac cattgaccgt taaaggtcag gcgtaccgtc ccgattgggc
    45061 cgttacgttg tttaccgata ataatttccg cgatgccttt taaatcactg ttttcgtgat
    45121 acacctcatc acgatagata aacatgatca agtccgcatc ctgctcgata gagccagatt
    45181 cacgcaggtc ggagttgacc gggcgtttgt cggcacgttg ttccagagaa cggttcaact
    45241 gggacagcgc caccaccggc acgttcagtt ctttcgccag tgctttcagc gagcgggaga
    45301 tttctgcaat ttccagcgta cggttatcgg aaagcgccgg tacgcgcatc agttgcaggt
    45361 agtcgatcat gataagcccg atgccgccgt gttcacgggc aatacggcgt gcgcgggaac
    45421 gcacttccgt tggcgtcaag ccggaggaat catcgatata gatattacgt ttttcgagca
    45481 aaatacccat ggtgccggaa atgcgtgccc agtcttcatc atcgagctgc ccggtacgga
    45541 ttttagtctg gtcaacgcgc gacagcgacg ccagagaacg catcatgatc tgttctgatg
    45601 gcatctccag cgagaagata agcaccggtt tatcctgcaa catcgccgcg ttttcgacga
    45661 ggttcatcgc aaatgtcgtt ttacccatcg acggacgcgc ggcaacgatg atcaaatccg
    45721 acggctgcaa gccagcggtt tttttgttga gatcgtcata accggtattt accccggtaa
    45781 cgccatcgtg tggctgctga aacaactgct caatacgcgc cacggttgcg tcgagcacat
    45841 cggcgatgtt cttcggccct tcgtctttgt ttgcacgact ttcggcaatt ttaaagacgc
    45901 gggattcagc aaggtcgagc agatcttcac tggtacgccc ttgtggatca aaaccagctt
    45961 cggcaatctc gttagcaacg gagatcatct cacgaacaac ggcacgttcg cgcacgatat
    46021 ccgcatatgc actgatgttc gccgcacttg gcgtattttt tgacagctct gccagataag
    46081 caaaaccacc gacgctatcg agttgcccct ggcgttccag cgattccgca agggtaatca
    46141 gatcgatagg actaccgctt tcctgcaaac gcgccatttc agtaaagata tgacggtgtg
    46201 ggcgggtgta aaaatcgtct gccaccacac gctcggctac atcatcccag cgttcgttat
    46261 ccagcattaa accgcccaac accgactgct ccgcttcgat cgagtgcgga ggcactttca
    46321 gcccggcaac ttgtggatcg cgttcgcggg gttcagcctg ctgtttgttg aagggtttat
    46381 ttcctgccat agtgaatgga gttaccgaga taaagaatgg gtcgaaactt taccatatga
    46441 agcagaccct gacgatacgt tctggaggac acatggcaac acgaattgaa tttcacaagc
    46501 acggtggccc ggaagtactt caagccgtag agttcactcc tgccgatccg gcggagaatg
    46561 aaatccaggt cgaaaataaa gccatcggca tcaattttat cgacacatat atccgcagcg
    46621 gcctttaccc gccaccatcg ttacccagcg gattaggcac cgaagcggca ggcatcgtga
    46681 gtaaagtcgg cagtggtgta aagcatatta aggcaggcga tcgcgtagtc tatgcacaat
    46741 cggcattagg cgcttacagc tctgtgcata acattaatgc ggataaagcg gcgattctgc
    46801 ctgcggcaat ttcttttgag caagctgcgg catccttcct gaaaggctta acggtttatt
    46861 atctgctgcg caaaacctat gaaattaaac ccgatgaaca attcctgttc cacgcagcgg
    46921 ctggcggcgt tggcttgatt gcctgccagt gggcaaaagc ccttggcgcg aaacttatcg
    46981 gcaccgtagg aaccgcgcaa aaagcgcaga gtgcgctaaa agcgggcgca tggcaggtta
    47041 ttaactatcg tgaagagaat ctggtcgagc gattgaaaga gatcaccggt ggtaagaaag
    47101 tgcgtgtggt gtacgattca gtgggcagag acacctggga acggtcgctg gattgcctgc
    47161 aacgccgcgg cttaatggtg agttttggca actcatcagg tgcggttacc ggtgtgaact
    47221 taggcattct caatcagaaa ggctctttgt atgtgacacg cccttccctg caaggctata
    47281 tcaccacgcg ggaggaatta accgaggcca gtaatgaact gttctctttg attgccagcg
    47341 gtgtgattaa ggtcgatgtc gccgagcagc agaaatatcc gctgaaggat gcgcggcgtg
    47401 cgcatgagat tctggaaagc cgggcgacgc agggttccag cctgttgatt ccataaaaga
    47461 aattgggctt ccacctggga agccctttct tttatagttc ggctgtatgt agggtacagc
    47521 tcgatgaatt tgtttgacgc gcaatagtga cagatttgat tatcaattcc tattttgttc
    47581 taaggataaa accttaggtt gtgatcatcc gcacaatccc ttagtaacgc cagcggtcat
    47641 aacgctgata tttcggcact tttggtgctt taatcgccct gatcacccac accaccgcaa
    47701 ccgccaacag cagccacggc agcaacttaa tcatcaatgc cagcataccg ccgaggaaca
    47761 taaaggccgt cgccacaatc agcgcggcga taatacccag caacgaaaca ccggtgacca
    47821 tcagcatgac aaaaaagcca attacaaaaa gtagttccag catgatgctc tcccaaatat
    47881 gaaatctctt gctggcatta caagaatcat gccaaaaata atctattgat ttaacagcaa
    47941 aacgccccgc gacggggcgc agggcgtggt gaatttgact actttttggt gaaaagttaa
    48001 cgcttatccg ccaccagttt gagcgcgtgt tccagcacat taatgtctgc acccgcttta
    48061 tgggcatttt cacttaaata acgccgccac tgccgcgcgc caggaatacc ctggaacaag
    48121 cccagcatat gccgggtaat atggccgaga tacgtaccct ggctgagttc acgctcaatg
    48181 tacggataca tggcgcgcac taccgccacc ggatcagcat cagtatccga ggaaccaaaa
    48241 atctctcggt ctaccgccgc cagaatcccc ggattctgat acgcctcgcg cccgaccatc
    48301 acgccatcca tatgttgcaa atgcgcttta gcttctccca gcgacttgat accaccgtta
    48361 atcgacatcg tcagatgagg aaagtcacgc ttcagttgat acacacgcgg ataatcgaga
    48421 ggcgggatct cacggttttc tttcggactt aacccagaaa gccaggcttt acgtgcgtgg
    48481 atgatgaaca tctcacactc acctttgccg gaaacggtgt tgatgaaatc gcagagaaat
    48541 tcatagctgt cctgatcatc gatgccaata cgcgtcttca ccgtcaccgg aatcgacacc
    48601 acatcgcgca tcgctttcac gcagtcggca accagctgcg cattacccat cagacacgca
    48661 ccaaacatgc cgttctgcac ccggtcagac gggcagccga cattcaggtt gatctcatcg
    48721 tatccacgcg cttctgccag cttcgcacac tgtgccagcg ccgccggatc actacccccg
    48781 agttgcaacg ctaccggatg ttcttcttca ctgtacgcca ggtaatcacc tttaccgtga
    48841 ataatcgccc ctgtggtcac catttcggta tacagcaacg tattgcggga aagcagacgc
    48901 aagaaatagc ggcaatgtct gtccgtccag tcaagcatcg gggcaatcga gaaacggtaa
    48961 gcgggataaa cagtgtgtga ttctgctggc atggcgactt tttaagcatc tggtaaattg
    49021 ggggcgctac tatagcataa cgaatatgcg atcaggcaat gtgacagcca tgacaccctg
    49081 ttctcggcgt ttaagtgaga actatggtaa agtaaggaca ttcttaaccc cactgtcgag
    49141 gtgcccaatg gaaaagacca caacgcagga gttattagcg caggctgaaa aaatctgcgc
    49201 acagcgtaat gtgcgcctga ccccacagcg cctcgaagtg ttgcgcctga tgagtctgca
    49261 ggatggcgct atcagcgctt atgatctgct tgatttgctg cgcgaagctg aaccgcaagc
    49321 caagccgcca acggtttatc gcgcgctgga gtttctgctt gaacaaggtt ttgtgcataa
    49381 ggtggaatcc accaacagtt atgtgctctg tcatctgttc gatcagccca cccatacttc
    49441 agccatgttt atttgcgatc gctgcggcgc ggtgaaagaa gaatgtgctg aaggcgtgga
    49501 agacatcatg catacgctgg cggcaaaaat gggttttgcc ctgcggcata atgttattga
    49561 agcacatgga ttatgttcag catgtgtaga agtagaagcg tgtcgtcatc ccgaacagtg
    49621 ccatcatgac cactctattc aggtgaaaaa gaaacctcgc taaaagggtg tacatccttg
    49681 tacatgtcgg gcaggaggga ttaattacca gcggtaatca tggcgttttt cccaactatc
    49741 aacctctttt tctgcctgat ctttctgata accgtagcgt tcctggattt tacccactaa
    49801 ctgatcacgt tttccttcaa tgatcgtcat atcatcatcg gtcagtttgc cccattgctc
    49861 tttcacttta cctttaaact gtttccagtt accgccggct tcatctttat tcataatcaa
    49921 gacctcatcg ttaggttgtg aatgagagta cgttcacttt tcttctgaac gtgagattaa
    49981 gtgtagtcag caatttggct taggattatt tattcagaat ttttaaccgt cacgttgcga
    50041 caaaccaggt atcgttacgc cagtgacgcc gccagatagc cgccagagaa agcccgcgta
    50101 acgccagaaa gacggttaac gccagccaca aaccatgatt ccccagccac ggcagcgtaa
    50161 ggagcgtcag cgcaaaacct gcggcggcca ccgccatact gttacgcatt tcggcggcac
    50221 gcgttgcgcc tataaacatg ccgtccagca gataacacca gacgccgacc aacggcaaaa
    50281 tcacctgcca gataagatag cggtcagcca gctgctgaat ctgggttaac gacgtcagca
    50341 acgcaatgat gtgttcccct gccagcaaat aaaccaccga aaacagtaac gctacgatcc
    50401 ccgactggcg gcacgctgcc cgccagacat ccagcaattg actaccgtcg cgcgcaccgt
    50461 atgcctgacc ggaatgtgct tcaaccgcgt aggcaaaacc atccagcgca taggcggtaa
    50521 aggtgagtag cgtcatcaga accgcgttaa cagcgataat gtcactcccc agtcgcgcgc
    50581 caagtacagt gatcgcgccg aaacagagtt gcagcaacag cgagcgcagc atgatatcgc
    50641 ggttaagcgc cagcaagtga cggaagtttc ctcgccaggc tgttttcagc atttcgccgg
    50701 agattccgcg tagtttgaga attttacgca ccattagcag accaatcagc aatgttgcat
    50761 attccgcaat aaccgtcgcc agagccgcgc cctgcacgtt catatgcagc cccatcacca
    50821 gccagacatc cagcacaatg ttgaggatat tgccgaccac taacaaaatt actggcgcac
    50881 gggcatattg cacgccgagt aaccaaccaa gtaataccag attcgccagc gacgccggtg
    50941 cgcttaacca gcggatttca agaaagcgtc gcgcctgttc caggactgct tcactaccgc
    51001 caacaatatg cagcgccaga tcgataatcg gcgtacgcag cagcgcaatt aacgccccag
    51061 cccccaacgc caacagcaac ggttgcacca gcgcacgggc taatgcctga ggatttttag
    51121 caccataagc ctgtgcagtc agcccggtgg tgctcatgcg taaaaacagc aacaacataa
    51181 agagaaagct ggtcgccgtt gcaccaaccg ccacgccgcc caaataaacc ggactatcaa
    51241 gatgaccaat taccgccgta tcgaccagtc ccagcaacgg aacggtgata ttggagaaaa
    51301 tcatgggtaa ggcgagatgc cagagtgctt tatcagatga agtgaggaat gccatgcaga
    51361 caagcctgat gaagagagat gaaaaacaaa ccgcgatacc aggcggcatc gcggtctcag
    51421 agatatgtta cagccagtcg ccgttgcgaa taaccccaac cgccagccct tcaatggtga
    51481 agctctgctg acgaaggtca acgacaattg gtttaaactc gctgttttct ggcaacagtt
    51541 cgactttatt gccctgtttt ttcaggcgct taacggtaac ttcgtcatca atacgtgcga
    51601 caacgacctg accgttacgt acatcctgag ttttatgcac tgccagcaaa tcgccatcca
    51661 taatgccgat atctttcatc gacatcccgc tgacacgcag caggaaatca gcattcggct
    51721 tgaacaagga aggatcgacc tgataatgac cttcaatatg ctgttgcgcc agaagcggtt
    51781 caccggcagc cacacgacct accagcggca acccttcttc ctcttcctgc aataaacgaa
    51841 tcccgcgtga tgcgccggaa acaatttcaa taacgccttt gcgtgccagc gccttcagat
    51901 gttcttcagc cgcgtttggg gaacggaacc ccaaacgctg cgcgatttcc gcacgcgtcg
    51961 gcggcatacc tgtctggctg atgtgatcac ggatgagatc aaacacctct tgttgcctgg
    52021 ccgttaacgc tttcattccg ccccctgggt gtatatacag ttatgctgtg agtatataca
    52081 gcaaaaggcg attttggaac cataaactgc acaataaacc agagatttat cgaaatctgg
    52141 aagggttatc caaaatgcga ccataacagg atgcaccagg taatcacggc gacgataatg
    52201 gcaatcagca ctgcggcgga tcccatatct ttagcgcgtc cggaaagctc atggtattca
    52261 gagccaattc ggtcaaccac agcttcgatg gcgctattga gaatttccac aatcatcacc
    52321 agcatcacgg agctgataag cagcacgcgg gtaatcgcgt ccacatccag ccagcaggcg
    52381 ataaccaccg ctaacaatac cgctacgcct tcctgacgga atgccgcttc gttgatccat
    52441 gcagcgcgta aacctttcca ggaatagcca gcagctttaa taattcggat gaatccagtg
    52501 gtattattgg ccattgaaag aaccttttta cattatgagc gtcaatatca gtgtaccgtt
    52561 aaatccgatg attcgggttt gcgtaacgcc gcgcgaaaca tgagcggata ccacagaatt
    52621 tcccatgact ttctgctatc cttgccgcgc atttgcatta ttaaccagag gctttacatc
    52681 gtttatgtcc ggctggccac gaatttacta caaattactg aatttaccat taagcatcct
    52741 ggtaaaaagc aagtctattc cggcggatcc tgccccggaa ctggggctgg atacctctcg
    52801 tccaattatg tacgttttac cgtacaactc gaaagctgac ttgctgacgt tgcgcgccca
    52861 gtgtctggca catgacctgc ctgacccgtt agagccgctg gaaatcgacg gcacgctact
    52921 gccgcgctat gtgttcattc acggcgggcc gcgtgtgttc acctattaca cgccgaaaga
    52981 agagtctatt aagctgttcc acgactatct cgatttgcac cgcagcaacc caaatctgga
    53041 tgtgcagatg gtgccagtat cggtgatgtt tggtcgcgcg ccggggcgtg aaaaaggcga
    53101 agtgaacccg ccgctgcgta tgcttaacgg cgtacagaaa tttttcgctg tactgtggct
    53161 cggtcgcgac agttttgtgc gtttctcgcc gtcagtttcg ctgcgccgta tggcagatga
    53221 acacggcacg gataaaacta tcgctcagaa actggcgcgc gtagcacgta tgcactttgc
    53281 ccgtcaacgt ctggctgccg tgggtccacg tctgcctgct cgtcaggatc tgtttaataa
    53341 gctgctcgcc tcccgcgcca ttgcgaaagc ggtagaagat gaagcacgca gcaaaaaaat
    53401 ctcccatgaa aaagcgcagc agaacgcgat tgcgctgatg gaagagattg cggcgaattt
    53461 ctcttacgag atgatccgcc tgactgaccg cattctgggc ttcacctgga accgacttta
    53521 ccagggtatc aacgtccata acgccgagcg cgttcgccag ctggcccacg acggccatga
    53581 gctagtgtat gtgccttgcc accgcagtca catggactac ctgctgcttt cttacgtgct
    53641 gtatcaccag gggctggtgc cgccgcatat cgccgccggg atcaacctga acttctggcc
    53701 ggccgggccg attttccgcc gtctgggggc gttctttatt cgccgtacgt ttaaaggcaa
    53761 taaactttat tccaccgttt tccgtgagta tctcggcgaa ctgttcagcc gtggttattc
    53821 cgtcgagtat ttcgtggaag gcggtcgttc ccgtacgggg cgtttgctgg atccgaaaac
    53881 cggtacgcta tcgatgacca ttcaggcgat gctgcgtggc ggcactcgtc cgattacgct
    53941 gattccgatc tatatcggtt atgagcacgt catggaagtg ggtacttacg ccaaagaact
    54001 gcgcggtgcg acgaaagaga aagagagcct gccgcagatg ctgcgtggtt taagcaagct
    54061 gcgtaatctc ggtcaggggt acgtcaactt cggtgaacca atgccgttga tgacctacct
    54121 taaccagcac gtaccagact ggcgtgaatc tatcgatccc atcgaagcgg tgcgtccggc
    54181 ctggttaacg ccgacggtca ataatattgc tgccgatctg atggtacgca ttaacaacgc
    54241 aggcgcggca aacgccatga acctgtgctg tactgcgctt ctggcgtcac gtcagcgctc
    54301 actcacccgc gagcagttaa ccgagcaact caactgctac ctggatctga tgcgcaacgt
    54361 gccttactcc acggactcta ccgttccttc agccagcgcc agcgagctta tcgatcacgc
    54421 gctgcaaatg aacaagtttg aagtcgagaa agacactatc ggcgacatca tcattctgcc
    54481 gcgcgagcaa gcggtgctga tgacctacta tcgcaacaac attgcgcata tgttggtgct
    54541 gccttctctg atggcggcaa ttgtcaccca gcatcgccac atctcccgcg acgtattgat
    54601 ggagcacgtc aatgtgcttt acccaatgct gaaagcggaa ctgttcctgc gctgggatcg
    54661 cgacgagtta ccggacgtta ttgatgcgct ggcaaatgag atgcaacgtc aggggctgat
    54721 taccctgcaa gatgatgagt tgcatatcaa cccggcgcat tctcgcacgc tacagctgct
    54781 ggccgcaggc gcgcgcgaaa cgctgcaacg ttatgccatc accttctggc tgttgagtgc
    54841 caacccgtcg atcaaccgcg gtacgctgga aaaagagagc cgcaccgtcg cgcaacgtct
    54901 ctccgtgctg cacggtatca acgcgccgga gttcttcgac aaggcggtgt tcagttctct
    54961 ggtgctgaca ctgcgtgatg aagggtatat cagcgatagc ggcgacgccg aaccggcaga
    55021 aacgatgaag gtatatcagt tgctggcgga gttgattaca tcagacgtgc gtttgacgat
    55081 tgagagtgcg acgcagggcg aagggtaatc agtgagaatt gccggatgcg acgcaaacgc
    55141 cttattcggc ctaccatgat ctgcaaattc aataaattgc gatatgttgt gtaggcctga
    55201 gaagcgcagc gcatcaggca acccagaaga aagccggatg atcatccggc ttttttacat
    55261 catcagaaat gccagtaact cattgccagc cctaaaaaca gtaccagacc aacatagtta
    55321 ttattcataa atgctttaaa gcaggcttca cgctcgcggt tggcaatcag tttttgttga
    55381 taaacaaaca gcgcgccagc taccagaatt gaccagtaat atccccagcc taagccattt
    55441 aactcaccga tgatcgccat cagcgctagt acgccaatct gcaaaatacc aataatcaat
    55501 ttatcgtatt ggccgaacag gattgcggtg gatttaatgc caatcttcac atcatcatcg
    55561 cggtcaacca tcgcatactg cgtgtcgtaa gccaccgccc agagaatatt ggcgaggaac
    55621 attaaccagc aactcaatgg caccgactca ctcacagcag caaaagccat tggaatcgac
    55681 cagccaaacg ccgcgcccag caccacttgc ggtagatggg tataccgctt cataaacggg
    55741 tacacccacg ccagcgccag cgcggcaatc gacaacagaa tagtcatcgt attcagcgtc
    55801 agcaccagta aaaatgaaat cagtaccagc actacaaaca gcgcgcgcgc ctctttctcc
    55861 gttaccgcgc cgctgggaag tggtcggttc gccgtgcgct taacatgacc atcaaacttg
    55921 cggtcagcat aatcattcac cacgcaacca gcagcgcgca tcagccagac gcccgcgaca
    55981 aacaccgcca ggatccagag ctggggaacg cccggtgtcg ccacccacaa cgcccataat
    56041 gttggccaga gcagcagtaa cgcgccaatt ggcttatccg tacgcattaa gcgatgaaac
    56101 gccagcagct tattctgcgt cagactccac tccatatttt tttcctctta gtacaacggt
    56161 gacgccggta aaaacagttc tgttagcaac agcggtttac cgcttaatcg caggcgggaa
    56221 cgtcgccccc acagtccggc atcacggcct atctcaataa agtcccgggt taatgtcgat
    56281 gatgtgaaca gatagcgtcc taacggcgtt ttacctaatt tttgtaacgc cagctccggc
    56341 ccgcttaacg ttgacacagg aacgacggta cgaccggcaa gccacggttc accatcggca
    56401 cataacaaaa tttcacgtaa ccagtaacga gactctttcg gcagcagtgg cagttcttcg
    56461 gggatttcat tctgctcgac aaacccttcg cggatcatcg tcacgctcac cgtttttccc
    56521 tgctgttcaa aacgttttgt catggaatct tccagcaaca gccagtcgag cagttgcggc
    56581 tccagggcag ggatctctgt aaaatagcgc agcgcacgca gttgcgttaa cgcggggtgt
    56641 gacatgccga actctccgtt acataacgta aaggcattgt atcgcagaaa aatatttacg
    56701 ggagatgtaa agcaaattaa gtgaactaat gcgcaacaaa aacgcaacaa tagtacagga
    56761 ggagaaaaaa aggtgcgcca ggagacgcac cagttgttgc aaaatcagca ctgtggggcg
    56821 taattacccc ttgcctttta cactgctgat aaaggtggaa cgggcagatg tcgaccccag
    56881 gcgttcagct tcatcaagca gttttaacgc cttatcaaca tcacctttcg cgacagcgtt
    56941 tttgatcgcg gtattaaaat aactttcggt gtcgttgagc atcggctcgc ttttcttcac
    57001 cggtgccgga gcgggtgcag ccacagctgg tgccgccgtg ttgcctaccg taaccggagc
    57061 tggagcggaa gaaccaaata gtggtcctac caacacgctg gagctggagt tcgttttcac
    57121 tttcagtttc agtaagccat cggtggtatg acgagcaacc ggatcgggaa tatccgggat
    57181 cgagttaccg acgcctttgg cataggcttt agccgggtcg agcagttgag tcgtctgctg
    57241 gagatctttt tccgtggtaa agaccagaac ataaagtttt tgctgcccca acgccggtgt
    57301 caggcgcata acgccttcca gccgatctgc actcatcacg ccgggttcct ggtaggtgaa
    57361 ataactgctg gggaagaagg ctgatggggt catgttctga tcaagaatca gcacattcgg
    57421 cgcaaatacg ctggtttgtt tgttcacttc gctggtcagc gttagggtca gttcgccaat
    57481 gtttgccggg acgctgtacg cagcaaccgg acctctgatg ccgggaacgt tcagttgttg
    57541 gccgccagtc gccagttggg tggtctgggt tttagattga tcgaccggtg tccaggtgag
    57601 ttgttgcagc gcggcagatg gaatggctgg cgcgtcgctg gtgttttgcg gtacgtagtt
    57661 aacatcggca aggctgattc caggcgcgct tgccagcaac cctgctgata aacagagggc
    57721 gatgagactt ttattcattt tcattgtttc acctcaaaat ctggagctca gcggtagcca
    57781 ggcaatagcg cgctaaaccc gataatcaga ggggctttcg ccccttaaag ctgtttttat
    57841 gccggatgcg gcgtaaacgc cttatccggc ctactttcgg cagcagttgt aggcctgata
    57901 agacgcaaca gcgtcgcatc aggcattggt tgccggatgc ggcgtaaacg ccttatccgg
    57961 cctactttcg gcagcagtta taggcctgat aagacgcaac agcgtcgcat caggcattgg
    58021 ttgccggatg cgacgcaagc accttatccg gcccaggttt tgctattacc accagatttc
    58081 catctgggca ccgaaggtcc actcgtcgct gtcgccacga ccgaagctac cgccgttgaa
    58141 atcagccgac acagccttgc cgtagtatgg gttggttgaa gagctaccgg tgtagtcata
    58201 accccatttc tcatcccact tggcgtaggt tgcgaagaca cgaatagccg ggcgtgacca
    58261 gatgctgtcg ccagcctgcc attgttgtgc aagggtaatt ttgtactgat tgttcttgtc
    58321 gccggtgcgc tgggattcga cgttgtcgta gccgatttcc atcacggtgc tcatgattgg
    58381 cgtccacttg tacatcgggc gaataccgac ggtccaccac ttggtgccgt tgtcgttatc
    58441 ccagttgata tcctggtaca tacccacgta catcatgtcc cagttgtcgc ccatggagat
    58501 cgcaccgtgg tcgaggatac gcagcatgtg accgttgttg ttgatattgt aggcaaattt
    58561 ttcgttatca aacgcgacgc cagaaccctg cgacagacct ttaccctgcg aggtcatcga
    58621 gtcagtagcg tactgaacaa caaacttgtt aaagcccttc aggacactct gagtatgttc
    58681 agcagtgaat aaccagccgt ctttcgatgc gccatcaacc agacgatagt tatcacgcag
    58741 gttggcacga ccgtagtcga cacccagttc taatgtgccg cccgggttga tttccatctg
    58801 cgctaaacgc acatcgaaaa cgtcgttcgc ggtttcgttg gtatagtcat aaatattgtt
    58861 gctggcgaaa gaggaagaac cacccgcttc agaggagcgg gttgctgcca gagagagttt
    58921 accgaagcca acatcgatgt tttccagacc ggcaccagga ccagaaatat cccagtagta
    58981 gaagtcgatc atatgaacgt catgacgttg gtagaagcgc ttacctgccc agatggtgga
    59041 gcctggcagc cattcgatca ggtttttacc ctgcacgttt gcttcacgga aggccggatc
    59101 ggtagcttcc cagtcattct gttgcgcgac ggaataggcc acgttagtgt cgaaatagaa
    59161 gctcttatcg ccctctttcc acacttcctg acccaatttt aattcagcat aagtttcaca
    59221 ttcgttgcca agacggtatt tactttgagc accggtagtc tggaaacact gttgttcgcc
    59281 gccgctacct gtccagccaa taccggaacg tgcatagccg tggaaatcaa cagccattgc
    59341 ctgagcagac attacgcccg ctgcgacggc aaccgccaga ggaagtttgc gcagagtaat
    59401 catcattcta tctcctgagt cattgctttt cttttttcac atcacctgtg acaggctttg
    59461 tgtgttttgt ggggtgctta aacgcccggc tccttatgca gtcgacgaca tgcagtgcca
    59521 tcctcacgga acagatggca acgctctggc ggcaggccga tagcgaatgt ggcaccttct
    59581 tctaccaaca ccacgtcgtt ctggcggtac accaggtttt gacgaatgga agggatctgg
    59641 atatggattt gcgtttcgtt gccgagttgc tcaacgacct gaacttcacc ctcaaggatg
    59701 acgtcagcga tatcactcgg cagtagatgc tccgggcgaa tacccagtga catattggct
    59761 ccaacctgga catcacggct ttcgaccggt agccagactt gctgacgatt cggcatcggc
    59821 agctccacct gcacttgatc gattgcggtg gcggtcactt ttactggcag gaagttcatc
    59881 tttggcgaac cgataaatcc ggcgacaaaa cggtctgccg gatagtgata cagctccagc
    59941 ggtttcccaa cctgcgccac gcgaccagcg tccagcacca cgattttgtc ggccagcgtc
    60001 atcgcttcga cctgatcgtg ggtgacgtaa atcattgtgc ggcccaggcg tttatgcaga
    60061 cgggagattt cgatacgcat ttgcacacgc agtgcagcat cgaggttgga gagcggttca
    60121 tcgagcaaaa atacgcttgg ctcggccacc agcgtacggc caatcgccac acgctgacgc
    60181 tgaccaccgg agagcgcttt cggtttgcga tccagcaaat gcgccagttg cagcacttcc
    60241 gcaacctggt taacttgctg gttaatcact tcttttttcg cgcccgccag tttcaggcca
    60301 aatgacatgt tttctgctac tgacaggtgg ggatagagcg cgtaagactg aaacaccata
    60361 ccaacgccgc gttctgcagg cggagtgtca ttcatccgct tctcaccgat gaacaggtcg
    60421 ccgctggtga tcgtctcaag cccggcaatc atgcgcagta aagtcgattt accgcagcca
    60481 gacggtccga caaacaccac gaattcacct tcatggatat cgagattgat atctttcgat
    60541 accacgacct cgccccaggc tttcgttaca ttttgcagct gtacgctcgc catgcccttc
    60601 tccctttgta acaacctgtc atcgacagca acattcatga tgggctgact atgcgtcatc
    60661 aggagatggc ttaaatcctc caccccctgg cttttttatg ggggaggagg cgggaggatg
    60721 agaacgcagc ttctgtgaac taaaccgagg tcatgtaagg aatttcgtga tgttgcttgc
    60781 aaaaatcatg gcgattttat gtgcgcatct ccacattacc gccaattctg taacagagat
    60841 cacacaaagc aacgatgggg cgtaggggca aggaggatgg aaagaggttg ccgtataaag
    60901 aaactagagt ccgtttaggt gttttcacga gcacttcacc aacaaggacc atagattatg
    60961 aaaataaaaa caggtgcacg catcctcgca ttatccgcat taacgacgat gatgttttcc
    61021 gcctcggctc tcgccaaaat cgaagaaggt aaactggtaa tctggattaa cggcgataaa
    61081 ggctataacg gtctcgctga agtcggtaag aaattcgaga aagataccgg aattaaagtc
    61141 accgttgagc atccggataa actggaagag aaattccctc aggttgcggc aactggcgat
    61201 ggccctgaca ttatcttctg ggcgcacgac cgctttggtg gttacgctca atctggcctg
    61261 ttggctgaaa tcaccccgga caaagcgttc caggacaagc tgtatccgtt tacctgggat
    61321 gccgtacgtt acaacggcaa gctgattgct tacccgatcg ctgtcgaagc gttatcactg
    61381 atttataaca aagatctgct accgaacccg ccaaaaacct gggaagagat cccggcgctg
    61441 gataaagaac tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca agaaccatac
    61501 ttcacctggc cgctaattgc ggctgacggg ggttatgcgt tcaagtatga aaacggcaag
    61561 tacgacatta aagacgtagg cgtggataac gctggcgcga aagcgggtct gaccttcctg
    61621 gttgatctga ttaaaaacaa acacatgaat gcagacaccg attactccat cgcagaagct
    61681 gcctttaata aaggcgaaac agcgatgact atcaacggcc cgtgggcatg gtccaacatc
    61741 gacaccagta aagtgaatta tggtgtaacg gtactgccaa ccttcaaagg tcaaccatct
    61801 aaaccgttcg ttggcgtgct gagcgcaggt attaacgccg ccagtccgaa caaagagctg
    61861 gcgaaagagt tcctcgaaaa ctatctgctg actgacgaag gtctggaagc ggtcaataaa
    61921 gacaaaccgc tgggtgcagt agcgctgaag tcctatcagg atgaacttgc gaaagatcca
    61981 cgtattgccg ccaccatgga taacgctcag aaaggtgaaa ttatgccgaa catcccgcag
    62041 atgtccgctt tctggtatgc cgtgcgtact gcggtgatca acgccgccag cggtcgtcaa
    62101 actgtggatg ctgcgctgaa agatgcacag actcgtatca ccaagtaatg ctgtgaaatg
    62161 ccggatgcgt cgtgaacgcc ttatccggcc tacgaaattg aaacgtatgt aggcctgata
    62221 agacgcgtca gcgtcgcatc aggcagttgt tgtcggataa ggtatgaaag ccttatccgt
    62281 cctggaatga ggaagaaccc catggatgtc attaaaaaga aacattggtg gcaaagcgac
    62341 gcgctgaaat ggtcagtgtt aggtctgctc ggcctgctgg tgggttacct tgttgtttta
    62401 atgtacgcac aaggggaata cctgttcgcc attaccacgc tgatattgag ctcagcgggg
    62461 ctgtatattt tcgccaatcg taaagcctac gcctggcgct atgtttaccc gggaatggct
    62521 ggaatgggat tattcgtcct cttccctctg gtctgcacca tcgccattgc cttcaccaac
    62581 tacagcagca ctaaccagct gacttttgaa cgtgcgcagg aagtgttgct agatcgctcc
    62641 tggcaagcag gcaaaatcta taattttggt ctttatccgg cgggcgatga gtggcaactg
    62701 gcgctcagcg acggcgaaac cggcaaaaat tacctctccg acgctttcaa atttggcggc
    62761 gagcaaaaac tgcaattgaa agaaagcgcc acgcagcctg aaggcgagcg cgcgaatctg
    62821 cgcgtgatta cccagaatcg tcaggcactg agtgacatta ccgccattct gccggatggc
    62881 aacaaagtga tgatgagctc cctgcgccag ttttctggca cgcaaccgct ctacacactc
    62941 gacggtgacg gcacgttgac gaataatcag agcggcgtga aatatcgtcc gaataaccaa
    63001 attggctttt accagtccat taccgccgat ggcaactggg gggatgaaaa gctaagcccc
    63061 ggttacaccg tgaccaccgg ctggaaaaac tttacccgcg tctttaccga cgaaggcatt
    63121 cagaaaccgt tcctcgccat tttcgtctgg accgtggtgt tctcgctgat cactgtcttt
    63181 ttaacggtgg cggtcggcat ggttctggcg tgtctggtgc agtgggaagc gttgcgcggc
    63241 aaagcggtct atcgcgtcct gcttattctg ccctacgcgg tgccatcgtt catttcaatc
    63301 ttgattttca aagggttgtt taaccagagt ttcggtgaaa tcaacatgat gttgagcgcg
    63361 ctgtttggcg tgaagcccgc ctggttcagc gatccgacca ccgcccgcac gatgctgatt
    63421 atcgtcaaca cctggctggg ttatccgtac atgatgatcc tctgcatggg cttgctgaag
    63481 gcgattccgg acgatttgta tgaagcctca gcaatggatg gcgcaggtcc attccagaac
    63541 ttctttaaga ttacgctgcc gctgctgatt aaaccgctga cgccgctgat gatcgccagc
    63601 ttcgccttta actttaacaa cttcgtgctg attcaactgt taaccaacgg cggcccggat
    63661 cgtcttggca cgaccacgcc agccggttat accgacctgc ttgttaacta cacctaccgt
    63721 atcgcttttg aaggcggcgg gggtcaggac ttcggtctgg cagcagcaat tgccacgctg
    63781 atcttcctgc tggtgggcgc gctggcgata gtgaacctga aagccacgcg aatgaagttt
    63841 gattaaggga gataacaaaa atggcaatgg tccaaccgaa atcgcaaaaa gcacgtttgt
    63901 ttattactca cctgctactg ctacttttta tcgcggcaat tatgttcccg ctgctgatgg
    63961 tcgtcgccat ctcgctgcgt caggggaact tcgcgaccgg tagtctgatc ccggagcaaa
    64021 tctcctggga tcactggaaa ctggcgttag gttttagcgt ggaacaggct gatggtcgca
    64081 ttacgccacc gccattcccg gtactgctgt ggctgtggaa ctcggtaaag gtcgccggga
    64141 tttccgcgat tggcattgtg gcgctctcca ccacctgcgc ctacgctttc gcccgtatgc
    64201 gctttccagg caaagcgacg ctgctgaaag gaatgctgat tttccagatg ttcccggcag
    64261 tactttcact ggtcgcgttg tatgcgttgt ttgatcgtct gggtgagtac attccattca
    64321 ttggcctgaa tactcacggc ggcgtaattt tcgcgtatct gggtgggatt gcgctgcatg
    64381 tctggaccat caaaggttat ttcgaaacca tcgacagttc gctggaagaa gcggcggcgc
    64441 tggatggtgc gacaccgtgg caggccttcc gccttgtcct gttgccgctg tcagtaccga
    64501 ttctggcggt tgtgttcatc ctgtcgttta tcgctgccat tactgaagtt ccggtcgcgt
    64561 cgctattact gcgtgacgta aacagctaca ccctggccgt ggggatgcag caatacctca
    64621 acccgcaaaa ctacctgtgg ggtgactttg ccgccgctgc cgtgatgtct gcattaccga
    64681 tcaccatcgt cttcttgctg gctcaacgct ggctggtcaa cggcctgacg gcaggtggtg
    64741 tgaaaggtta aagatgttgt tctgccaatg ttatgccgct gcaccctcaa cttacgttat
    64801 cccaacttgt gactgttatt cggcgctcca cggagcgcct ttttttactc ccgcttcagc
    64861 cgtttcgaat tacacagcca cagggtgatc accagtaaca ggatcgctgc cgagtagatc
    64921 aacacatcca gtggcgattt atgatcgacg atgatcaagc gcacaatggc ggtgatccca
    64981 atatagacaa agtaacgtag cgggaagtga aaaccggact gaaagtactt cacaatcagc
    65041 gcgataaatt cgaaatagag aaagtaaacc accagccctt ctaccagctc atatttactg
    65101 gtttgttctg gcgcgaacag cacatcagcc agatgcaccg tttctttgcc gaggaagaca
    65161 accaaaatca ggccgaggca caacaggcca agattgagta cggtctgcaa aatggtggag
    65221 ataaactcca cgcgcggacg agagagtgac gtcatagcag tacctccttc acagtggcga
    65281 gcttcctgta taacgcaaaa atgtgacgga gatctatatt ttttgtgtat gttttgttca
    65341 gctcgattaa gtgggcatct cccatcaatg tctcatctgg caaaacacac agcccaaaaa
    65401 aatcacccat tcaggtgatt cccagccacc agcaatgcgc gaaaaatcga caatgttcct
    65461 ctggaacaaa tgaaacgtaa cctgataaag gtggtaaaaa tgaagcctta ttttgctgct
    65521 ttgatgttat cagtctctgt tcttcctgct tatgctggtc cgctcggtac tgctgataaa
    65581 gccgacctac cgcaaagcaa tgtctccagt ccaatgatgg cccagtcgct caggcaacca
    65641 gacctgcaac cgatatcgac ggacaggaag acagaatgtt ttcgccttta cacaccagat
    65701 cgcaaaccgg gggtgaactg cgttcctgat ggctcgacag gccattaaga atgaaggtgc
    65761 cagtgagtgc gaaaactggc acctttccca tgacttagcg gaaattgacg ctacggtcgc
    65821 tggtcatatc atacaactgg aacttacgcc caagttgctg accaccgtca cgcgtcagcg
    65881 gcgtccagcc aatcgccgca cggctgcgtg tcgggccaga cgagaagaga tccagcggta
    65941 cagagacata cacgcctttg gtgaagtccc cttcgccgta ctcctctttc gaaacattgg
    66001 tgatcgtggc atagcccccc actaccacgc cgctatcaaa gcgtttggcg atctccagcg
    66061 tgccgccttt atccccagcc agatactgcc cgacgctcgc tttcactaac acgtcctggg
    66121 cgaaagatgg cgtccagtac gcggtcagat gtccggtttt cacgctgtaa tcggtgaatt
    66181 tcatcatatc ttttgcgcta cgccagtcgc gctgtttaac gtagttggca tccagaccaa
    66241 acgcccagtt gctatccagc gggcgataca acacttctgc ccccacgccg ccaaacatgg
    66301 tttcgagata acctccgtag acctgaccgt agaagccgtt gcccagatgc tggaagtagt
    66361 tggcttgcag gttattcaca tagacatcgt tctgcacata ctcgcgcaca tgggtacgca
    66421 cgcgcggtaa gtgcgagtcc tgcggtggat tggtgtagtt aaacttgtcg tagttgttgg
    66481 caaggttagc aaacaggctg ccggtggtca gcagatggtc cgtcagccac aaatccgccg
    66541 ttcccatcac gcccagctga tacatgtaaa agttttccgg gccaccgacc gactggttca
    66601 gcaccggatc aatatgaaaa tcaaagcgcg atttgtcgat ataccagccc tgctcagtgg
    66661 acttcggaac cactggctcg acgcgttttt gcgccagctt cgtttcgtgg cccaacggct
    66721 ctccggcgag atgatttttc aggctggcga catcggtttc cgtcgtcacc tgcggcatgt
    66781 taaggcgatt ttccgtaatg cggatcgttt tgatcccatc cggtagatcg ttcatcacga
    66841 tccgattagc acggatgctc ccttcgcgcg gatcacgata tttcacctgc tcgccggtaa
    66901 catacagcgt atcgcctttc gcctggatct gcggatcggc cagtccggcg ttgtatttca
    66961 acagcgttaa ctgattcgcc accaccgagt gctgcaaaat ggcatcctgc ggctgcggtt
    67021 gatattgcgg gcgggcgtta tcgttgtagg acgggcgcag atcgttaaag ttggtgcgca
    67081 gcgtaacgcc aaacataaag gtgttgccac gttcatagct aaggttaacg tcggcccaat
    67141 cggtaacgcg ataaatcgcc ccgacgttaa acttactttt ttgctccagc ttcccggcaa
    67201 aatcctgctg ataattattg ccttcatact ccagtttcag gcgcagcggt tgccagggcg
    67261 tctggtattc cacgccgcca aacagtgagg ccgggccgtg gaacatctgg ctaccgtcga
    67321 tagatcccgc ctgtttgtag ctgttatcgc gatagcaata tttatcactg gctgaacaga
    67381 gcggattttt cacattaccg ctggtgccca gatatcccca gcccagaccg agcgtaaaat
    67441 cgaacggccc ccaggctttg ctggcaacaa gatattccgc atcaaacagc cccgtaccgc
    67501 cgatatcccg cgcaccaacc gccacttgcg gcagccagta actctcttcc cacaaacgca
    67561 gtttgagatc gaaggcttta tctttatacg tttgatcgcc agagaatgct tcgacgctgc
    67621 tgtactgccg ggtgcgcacg tcggtgtagc gcagcgttgt ttccagccac gggaagagtt
    67681 gcactgaagc tgagtaataa cggtactgat cgttgtcgcg atagttcagg ctcaactccc
    67741 cttcccgcgc catgcgtgcg gtgggcgttt gtaataatcc tacgccaccg aaatccgatt
    67801 gcgacggacc aatgagcgca ggatatgttt cgccgtagca agctgtgcta atgcccagcg
    67861 ccagtaagct aagcagatgt ctttttttca ttattggggt atccgctgcg tcagagtctg
    67921 aagaatgtcg gcgttaaggg catcaggtgt ctcactccag acggagtccg cgaggccaac
    67981 ataaataatg ctgccgggca tcggctctac gtgacgcttg ttccagtaag ccaccggcgc
    68041 tttttgcgtg cgtccgtccg ggtaaaccac ccacgcgtag ctgcgatccg caccgctgag
    68101 caggctctgg tcagagagat agctcgccac gtcgcgacct ggagtgaatg actgcttgcc
    68161 aggacgactg ataagcccga acaacgtgac cgtggacggc ggcgttccga cccacagcgt
    68221 gtagttgccc tgcaacggcg ggttaccgcg ttcggcaacg cggacgatat caggatcaag
    68281 attgatcttt tgcctgcccg tcactttcaa cgcctgaatt tgctggcgta aggcgttaat
    68341 cgcggcggca tcgtcggcgc tggagtctgc agccagttct gccagccgtg tcagcaacgc
    68401 ctgctgctga cgcaatgctg ccgccgtggc cagctcttcg ctaatcaccg ctccgggcca
    68461 ccaactgttt gccagccgtg gttggcctac cagatcgatt aaatgctctg cacccgtcag
    68521 cgttttggcc tcactgctgc cattgctgaa taccttgacg gttcccgccg cgaagaccga
    68581 tgacgccccc acactcaaaa tcaatgcgac aatagtttgt ttaatcattg ttttgccgcc
    68641 ttgatgagag tggttttcac cgggaaataa tccgcgccga gatactgttc cgactggcga
    68701 atttgccctt cgctgtcgat ccaatagcgg ttatgccagc gcgcctggtc ggtggagact
    68761 tcttcgtcca gcacgcgaac cggggtttca tcgctgccga ctttgacggt atcggtgcca
    68821 tcccatttga agactgagcg tgcggtggcg tagcgtacct gctggtactc ggtccagccc
    68881 atcgtgcgcg tccatgttgc accatcaaca atttgtgcgg gtttaatcag cgggtcagtg
    68941 gcaaggttat tcacttcaat caggttgtcg ccaccaagca aagtcttcac cagacgagca
    69001 tgttgtgtga cgagagtggc ctgatcctga gtgacccatt tttgttgtcc gtcttcagcg
    69061 aaggccagca ccacaaataa ctgcggcccg ccattaagct gcatgtactg gctggcgtag
    69121 ggcatatttt gaatatcatc gtccgtcagc tgtacgcctg gcgtgccgaa cagactgtcc
    69181 cacagtgaat tgcccagctc tttagtcgtg gctgaacagg cctgtaatag caggcagata
    69241 agaatgagtg caggtcgctt cacgactctt ctccagaggg gcagaataac cacaccggag
    69301 tgtggttatg gttaatcccc ttcatacttc aagcttcaga tgcgatgact cgaattattt
    69361 tggggataca tactttatta ctgggtactg gtggtcgtgg ttgtggtggt cccggtatta
    69421 gaaccatcac cgccaccggt tgccgcgagc gcgacaccca ccgccgagct tacggtgctg
    69481 acgctggtcg cggaggaact acccgccgaa accgacgttg ctgccgatcc tgccgcttcg
    69541 cccacctgta caggtgcagc ccacgcagaa gtcgccgcaa gcgcagatat ggcaaaaatg
    69601 ccatacagaa cttttttcat aacaatttcc cttcattgaa tgaatggaga tttacccaaa
    69661 acatttcggg cgggatcgag tatacacaac taaagcatgc gaattacggc gcggggaatt
    69721 agtgatggag tttt
//
LOCUS       DAZFJL010000005        66380 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFJL010000005 DAZFJL010000000
VERSION     DAZFJL010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.005"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..1032
                     /locus_tag="ACHJ8C_000323"
     CDS             <1..1032
                     /locus_tag="ACHJ8C_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFO5550243.1"
                     /translation="KKYTILASSLISGSVFAQTEISTANSSTVNASYVEHSAEKIISP
                     SDKLNNLFERNMSQPYILQKIGERTYYVQRYFYSTTFYVGDKGVLLFDAPEGRGKYLL
                     QAIRDVTPLPVTALVYSHYHVDHIGDSPFWNDEAKKEGVNLRIIASKATAEKMQFMNS
                     RLPVATQVLSKKDDQFKFEKQTIELHRFVKAGHTDDHSVWLLKQEKVAHSPDLLNPDQ
                     LPMMGFAVSDTLVYHDSNLRQVEMLDWKYFIGGHGNIGSHDDFKFQRQFLNDLRDTTI
                     KVRKEESFGKFMNKTANNHADFARAQREAIIKKVTEVLRPKYGHMYGYDASMPANIEM
                     AIRLVGSYY"
     gene            complement(1175..2539)
                     /gene="ygbN"
                     /locus_tag="ACHJ8C_000324"
     CDS             complement(1175..2539)
                     /gene="ygbN"
                     /locus_tag="ACHJ8C_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFO5550244.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WLVI"
     gene            complement(2628..3404)
                     /gene="ygbM"
                     /locus_tag="ACHJ8C_000325"
     CDS             complement(2628..3404)
                     /gene="ygbM"
                     /locus_tag="ACHJ8C_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO5550245.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSPQQ
                     IQQQLVQNHLTLALFNTAPGDINAGEWGLSALPGREHEARADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYQAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEKGLGWFDAWR"
     gene            complement(3409..4047)
                     /gene="ygbL"
                     /locus_tag="ACHJ8C_000326"
     CDS             complement(3409..4047)
                     /gene="ygbL"
                     /locus_tag="ACHJ8C_000326"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO5550246.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVTADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(4044..5306)
                     /gene="otnK"
                     /locus_tag="ACHJ8C_000327"
     CDS             complement(4044..5306)
                     /gene="otnK"
                     /locus_tag="ACHJ8C_000327"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFO5550247.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVKAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(5303..6211)
                     /gene="ltnD"
                     /locus_tag="ACHJ8C_000328"
     CDS             complement(5303..6211)
                     /gene="ltnD"
                     /locus_tag="ACHJ8C_000328"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFO5550248.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNATQVKQVLFGEKGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFGLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            6407..7174
                     /gene="ygbI"
                     /locus_tag="ACHJ8C_000329"
     CDS             6407..7174
                     /gene="ygbI"
                     /locus_tag="ACHJ8C_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFO5550249.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGRCIYLDAGT
                     TTLAIAQHLTHMEPLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKLDLSLLVAKNE"
     gene            complement(7225..7881)
                     /gene="pphB"
                     /locus_tag="ACHJ8C_000330"
     CDS             complement(7225..7881)
                     /gene="pphB"
                     /locus_tag="ACHJ8C_000330"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO5550250.1"
                     /translation="MPSTRYQKINAHHYRHIWTVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALDAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDTIKYVIAHADYPGKEYQFGKEIAESELL
                     WPVDRVQKSLNSELQKINGADFFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(7987..10548)
                     /gene="mutS"
                     /locus_tag="ACHJ8C_000331"
     CDS             complement(7987..10548)
                     /gene="mutS"
                     /locus_tag="ACHJ8C_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO5550251.1"
                     /translation="MSTIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMERQQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLENVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            10834..11178
                     /gene="ygbA"
                     /locus_tag="ACHJ8C_000332"
     CDS             10834..11178
                     /gene="ygbA"
                     /locus_tag="ACHJ8C_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO5550252.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            complement(11217..11645)
                     /locus_tag="ACHJ8C_000333"
     CDS             complement(11217..11645)
                     /locus_tag="ACHJ8C_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HFO5550253.1"
                     /translation="MAVSARNQLTGTVSAVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(11690..13768)
                     /gene="flhA"
                     /locus_tag="ACHJ8C_000334"
     CDS             complement(11690..13768)
                     /gene="flhA"
                     /locus_tag="ACHJ8C_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO5550254.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFNTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(13842..14852)
                     /gene="hypE"
                     /locus_tag="ACHJ8C_000335"
     CDS             complement(13842..14852)
                     /gene="hypE"
                     /locus_tag="ACHJ8C_000335"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO5550255.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(14849..15970)
                     /gene="hypD"
                     /locus_tag="ACHJ8C_000336"
     CDS             complement(14849..15970)
                     /gene="hypD"
                     /locus_tag="ACHJ8C_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO5550256.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(15970..16242)
                     /gene="hypC"
                     /locus_tag="ACHJ8C_000337"
     CDS             complement(15970..16242)
                     /gene="hypC"
                     /locus_tag="ACHJ8C_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO5550257.1"
                     /translation="MCIGVPGQIRTIDGNLAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(16233..17105)
                     /gene="hypB"
                     /locus_tag="ACHJ8C_000338"
     CDS             complement(16233..17105)
                     /gene="hypB"
                     /locus_tag="ACHJ8C_000338"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO5550258.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(17109..17459)
                     /gene="hypA"
                     /locus_tag="ACHJ8C_000339"
     CDS             complement(17109..17459)
                     /gene="hypA"
                     /locus_tag="ACHJ8C_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO5550259.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            17671..18132
                     /gene="hycA"
                     /locus_tag="ACHJ8C_000340"
     CDS             17671..18132
                     /gene="hycA"
                     /locus_tag="ACHJ8C_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO5550260.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPVA"
     gene            18257..18868
                     /gene="hycB"
                     /locus_tag="ACHJ8C_000341"
     CDS             18257..18868
                     /gene="hycB"
                     /locus_tag="ACHJ8C_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO5550261.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCATVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            18865..20691
                     /gene="hycC"
                     /locus_tag="ACHJ8C_000342"
     CDS             18865..20691
                     /gene="hycC"
                     /locus_tag="ACHJ8C_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO5550262.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMNVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            20694..21617
                     /gene="hycD"
                     /locus_tag="ACHJ8C_000343"
     CDS             20694..21617
                     /gene="hycD"
                     /locus_tag="ACHJ8C_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO5550263.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            21635..23344
                     /gene="hycE"
                     /locus_tag="ACHJ8C_000344"
     CDS             21635..23344
                     /gene="hycE"
                     /locus_tag="ACHJ8C_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO5550264.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            23354..23896
                     /gene="hycF"
                     /locus_tag="ACHJ8C_000345"
     CDS             23354..23896
                     /gene="hycF"
                     /locus_tag="ACHJ8C_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO5550265.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYVIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            23896..24663
                     /gene="hycG"
                     /locus_tag="ACHJ8C_000346"
     CDS             23896..24663
                     /gene="hycG"
                     /locus_tag="ACHJ8C_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO5550266.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            24660..25070
                     /gene="hycH"
                     /locus_tag="ACHJ8C_000347"
     CDS             24660..25070
                     /gene="hycH"
                     /locus_tag="ACHJ8C_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO5550267.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            25063..25530
                     /gene="hycI"
                     /locus_tag="ACHJ8C_000348"
     CDS             25063..25530
                     /gene="hycI"
                     /locus_tag="ACHJ8C_000348"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO5550268.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPADIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQQIKDAVETVYQRLEGWEGNGGFAQLAAEE"
     gene            25573..26328
                     /locus_tag="ACHJ8C_000349"
     CDS             25573..26328
                     /locus_tag="ACHJ8C_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5550269.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     DQNWHFRKVGVAGVTLEKMPNEDDYWRLTAKDRAGKSWFVPVGVLQNMAGNAQFYRAD
                     LDRNGIQDLVIWRGVSGNGLAPSSFLILMTFNQQGRPCVFQVDGFYTANETGVDDLLD
                     LQGNGHTQLLDMQFDSGYWITSLYQVKEAKWQRVHGWFGKLSYPALTRFTYTSNRKLV
                     IKPVAGRNPQTKDLVLTQRCLIKGDVLDGVNQN"
     gene            26491..26802
                     /locus_tag="ACHJ8C_000350"
     CDS             26491..26802
                     /locus_tag="ACHJ8C_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO5550270.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            26799..27218
                     /locus_tag="ACHJ8C_000351"
     CDS             26799..27218
                     /locus_tag="ACHJ8C_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5550271.1"
                     /translation="MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(27310..27738)
                     /locus_tag="ACHJ8C_000352"
     CDS             complement(27310..27738)
                     /locus_tag="ACHJ8C_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4259 domain-containing protein"
                     /protein_id="HFO5550272.1"
                     /translation="MGSWGMEALESDEGLDLINWVEEQLQDDSTFDAESIVQRLSQHE
                     DLFGFQGDEEFLYDNNVIGLVELIIQKAAGEKITSSKQIDQLDNYRLTSIFSKKLQGR
                     LQTIDDTHEWIMLFEGRAREKAKAYLIEITDKLRVVKTTA"
     gene            28123..28650
                     /gene="hydN"
                     /locus_tag="ACHJ8C_000353"
     CDS             28123..28650
                     /gene="hydN"
                     /locus_tag="ACHJ8C_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO5550273.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            28803..31055
                     /gene="hypF"
                     /locus_tag="ACHJ8C_000354"
     CDS             28803..31055
                     /gene="hypF"
                     /locus_tag="ACHJ8C_000354"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO5550274.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGVMNTQI
                     VPDAATCPACLAEMNTPGERRWRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYIPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRGSGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHLWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIVAA
                     RWLAGEVQNG"
     gene            complement(31279..32412)
                     /gene="norW"
                     /locus_tag="ACHJ8C_000355"
     CDS             complement(31279..32412)
                     /gene="norW"
                     /locus_tag="ACHJ8C_000355"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO5550275.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIIGGGLIGSELAMDF
                     CRAGKMVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRCIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLSM"
     gene            complement(32409..33848)
                     /gene="norV"
                     /locus_tag="ACHJ8C_000356"
     CDS             complement(32409..33848)
                     /gene="norV"
                     /locus_tag="ACHJ8C_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO5550276.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIVYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVEEETSAATTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            34035..35549
                     /gene="norR"
                     /locus_tag="ACHJ8C_000357"
     CDS             34035..35549
                     /gene="norR"
                     /locus_tag="ACHJ8C_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO5550277.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGEAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYNFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            complement(35546..36511)
                     /gene="gutQ"
                     /locus_tag="ACHJ8C_000358"
     CDS             complement(35546..36511)
                     /gene="gutQ"
                     /locus_tag="ACHJ8C_000358"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO5550278.1"
                     /translation="MSEALLNAGRQTLMLELQEASHLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDDQRLVKGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            36765..37850
                     /gene="mltB"
                     /locus_tag="ACHJ8C_000359"
     CDS             36765..37850
                     /gene="mltB"
                     /locus_tag="ACHJ8C_000359"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO5550279.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            37995..38492
                     /gene="pncC"
                     /locus_tag="ACHJ8C_000360"
     CDS             37995..38492
                     /gene="pncC"
                     /locus_tag="ACHJ8C_000360"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO5550280.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            38572..39633
                     /gene="recA"
                     /locus_tag="ACHJ8C_000361"
     CDS             38572..39633
                     /gene="recA"
                     /locus_tag="ACHJ8C_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO5550281.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            39702..40202
                     /gene="recX"
                     /locus_tag="ACHJ8C_000362"
     CDS             39702..40202
                     /gene="recX"
                     /locus_tag="ACHJ8C_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO5550282.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            40331..42961
                     /gene="alaS"
                     /locus_tag="ACHJ8C_000363"
     CDS             40331..42961
                     /gene="alaS"
                     /locus_tag="ACHJ8C_000363"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO5550283.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFDAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            43196..43381
                     /gene="csrA"
                     /locus_tag="ACHJ8C_000364"
     CDS             43196..43381
                     /gene="csrA"
                     /locus_tag="ACHJ8C_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO5550284.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            43697..43789
                     /locus_tag="ACHJ8C_000365"
     tRNA            43697..43789
                     /locus_tag="ACHJ8C_000365"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43731..43733,aa:Ser,seq:gct)
     gene            43793..43869
                     /locus_tag="ACHJ8C_000366"
     tRNA            43793..43869
                     /locus_tag="ACHJ8C_000366"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43827..43829,aa:Arg,seq:acg)
     gene            43934..44010
                     /locus_tag="ACHJ8C_000367"
     tRNA            43934..44010
                     /locus_tag="ACHJ8C_000367"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43968..43970,aa:Arg,seq:acg)
     gene            44074..44150
                     /locus_tag="ACHJ8C_000368"
     tRNA            44074..44150
                     /locus_tag="ACHJ8C_000368"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44108..44110,aa:Arg,seq:acg)
     gene            44409..44975
                     /gene="yqaB"
                     /locus_tag="ACHJ8C_000369"
     CDS             44409..44975
                     /gene="yqaB"
                     /locus_tag="ACHJ8C_000369"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO5550285.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGYYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            44972..45400
                     /gene="yqaA"
                     /locus_tag="ACHJ8C_000370"
     CDS             44972..45400
                     /gene="yqaA"
                     /locus_tag="ACHJ8C_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO5550286.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGVSHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            45473..47029
                     /gene="gshA"
                     /locus_tag="ACHJ8C_000371"
     CDS             45473..47029
                     /gene="gshA"
                     /locus_tag="ACHJ8C_000371"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO5550287.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETVQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            47179..47694
                     /gene="luxS"
                     /locus_tag="ACHJ8C_000372"
     CDS             47179..47694
                     /gene="luxS"
                     /locus_tag="ACHJ8C_000372"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO5550288.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(47745..48857)
                     /locus_tag="ACHJ8C_000373"
     CDS             complement(47745..48857)
                     /locus_tag="ACHJ8C_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5550289.1"
                     /translation="MIWQLTDDKRWSALRQRFSWVEEMHHTPQDPEHHGEGDVGVHTE
                     MVLNALITLPEFQQLPTQQQEVLWAAALLHDVEKRSTTVQENGRIQSPGHARRGELTA
                     RQILWRDIPTPFVLREQIVALVRLHGLPLWLLERPEPERLLLTAAMRIDTRLLALLAR
                     ADLLGRQSPDQQSMLERIDLFELFCHEQQCWGKMRPFVSDSARWHYLTHEQSSPDFVP
                     WEAEPFEVILLCGLPGMGKDRYINEQCQGMDVISLDDMRRRINASPDDKTATGRIVQQ
                     AKEEARVFLRQKKPFIWNATNITRQLRSQLISLFTAYGARVKIVYLEVPWAQWKQQNA
                     RREYAVPEAVVMRMASRLEVPQPDEAHSVEYRMTDR"
     gene            complement(48854..49561)
                     /locus_tag="ACHJ8C_000374"
     CDS             complement(48854..49561)
                     /locus_tag="ACHJ8C_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HFO5550290.1"
                     /translation="MNTQRKYGRTWHYPFSPGTTSDDRINTDYWQDLQTITQLVHTEK
                     LDGENNCLNRYGVFARSHATPTQSAWTYKIRQRWQLLKNDLGDLELFGENLYAVHSIE
                     YRALEQDFYLFAVRCQDMWLSWEEVQFYAALFDFPCVPEISGPQPGNDEKSWQRDFLA
                     LANARGTFDPWDTQTWQPCTLEGIVSRNHDAFSVADFSHNVFKYVRKNHVKTTVHWKR
                     HWQRARMAHEFVYGEQS"
     gene            complement(49819..51357)
                     /gene="emrB"
                     /locus_tag="ACHJ8C_000375"
     CDS             complement(49819..51357)
                     /gene="emrB"
                     /locus_tag="ACHJ8C_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO5550291.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESLHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(51374..52546)
                     /gene="emrA"
                     /locus_tag="ACHJ8C_000376"
     CDS             complement(51374..52546)
                     /gene="emrA"
                     /locus_tag="ACHJ8C_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO5550292.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALTSSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(52673..53203)
                     /gene="emrR"
                     /locus_tag="ACHJ8C_000377"
     CDS             complement(52673..53203)
                     /gene="emrR"
                     /locus_tag="ACHJ8C_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO5550293.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(53294..53629)
                     /gene="ygaH"
                     /locus_tag="ACHJ8C_000378"
     CDS             complement(53294..53629)
                     /gene="ygaH"
                     /locus_tag="ACHJ8C_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO5550294.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTSRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(53619..54356)
                     /gene="ygaZ"
                     /locus_tag="ACHJ8C_000379"
     CDS             complement(53619..54356)
                     /gene="ygaZ"
                     /locus_tag="ACHJ8C_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO5550295.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(54480..55664)
                     /locus_tag="ACHJ8C_000380"
     CDS             complement(54480..55664)
                     /locus_tag="ACHJ8C_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5550296.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASMLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARSAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGSWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(55856..56848)
                     /gene="proX"
                     /locus_tag="ACHJ8C_000381"
     CDS             complement(55856..56848)
                     /gene="proX"
                     /locus_tag="ACHJ8C_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO5550297.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEDDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(56905..57969)
                     /gene="proW"
                     /locus_tag="ACHJ8C_000382"
     CDS             complement(56905..57969)
                     /gene="proW"
                     /locus_tag="ACHJ8C_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO5550298.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPSQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(57962..59164)
                     /gene="proV"
                     /locus_tag="ACHJ8C_000383"
     CDS             complement(57962..59164)
                     /gene="proV"
                     /locus_tag="ACHJ8C_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO5550299.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKYVGAVSIDSLKAALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(59521..60480)
                     /gene="nrdF"
                     /locus_tag="ACHJ8C_000384"
     CDS             complement(59521..60480)
                     /gene="nrdF"
                     /locus_tag="ACHJ8C_000384"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO5550300.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLLPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISQAQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(60490..62634)
                     /gene="nrdE"
                     /locus_tag="ACHJ8C_000385"
     CDS             complement(60490..62634)
                     /gene="nrdE"
                     /locus_tag="ACHJ8C_000385"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO5550301.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(62607..63017)
                     /gene="nrdI"
                     /locus_tag="ACHJ8C_000386"
     CDS             complement(62607..63017)
                     /gene="nrdI"
                     /locus_tag="ACHJ8C_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO5550302.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(63014..63259)
                     /gene="nrdH"
                     /locus_tag="ACHJ8C_000387"
     CDS             complement(63014..63259)
                     /gene="nrdH"
                     /locus_tag="ACHJ8C_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO5550303.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(63468..63899)
                     /locus_tag="ACHJ8C_000388"
     CDS             complement(63468..63899)
                     /locus_tag="ACHJ8C_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO5550304.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELVYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTEIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            63989..64732
                     /locus_tag="ACHJ8C_000389"
                     /pseudo
     CDS             63989..64732
                     /locus_tag="ACHJ8C_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            complement(64789..65118)
                     /gene="ygaM"
                     /locus_tag="ACHJ8C_000390"
     CDS             complement(64789..65118)
                     /gene="ygaM"
                     /locus_tag="ACHJ8C_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO5550305.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            65270..65614
                     /gene="ygaC"
                     /locus_tag="ACHJ8C_000391"
     CDS             65270..65614
                     /gene="ygaC"
                     /locus_tag="ACHJ8C_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO5550306.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(65651..66100)
                     /gene="alaE"
                     /locus_tag="ACHJ8C_000392"
     CDS             complement(65651..66100)
                     /gene="alaE"
                     /locus_tag="ACHJ8C_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO5550307.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT        15730 a        16959 c        17698 g        15993 t
ORIGIN      
        1 aaaaaatata caattctagc ctcttctctt atatctggta gtgtttttgc tcaaacggaa
       61 atttcgactg caaattcgtc tacagttaat gcaagttatg ttgaacattc agcggaaaaa
      121 ataatatcgc caagcgataa actcaataac ctttttgaaa gaaatatgtc tcagccttat
      181 attttacaaa aaattggcga gagaacttat tatgttcaac gctattttta ttccactacg
      241 ttttatgtcg gagacaaagg ggttttgctg tttgatgctc cggagggtcg cggtaaatat
      301 ttattacagg caattcgtga tgtgactcca ttacccgtaa cagcgctggt ttattctcat
      361 taccatgtgg atcacattgg cgatagtcca ttttggaacg atgaggcgaa gaaagaaggc
      421 gtgaatctgc gtattattgc cagtaaagcc actgctgaaa aaatgcaatt tatgaacagt
      481 cgcttacccg tcgccactca ggttttaagt aaaaaagatg accagtttaa atttgaaaag
      541 cagaccattg aattacaccg atttgttaaa gcaggacata ccgacgacca ttctgtatgg
      601 ttgctaaaac aggaaaaagt agctcacagc ccggatctcc tgaatcctga ccaactgccg
      661 atgatgggct tcgccgtatc ggatacgttg gtttaccatg acagtaattt gcgtcaggtt
      721 gaaatgcttg actggaaata ttttattggc gggcatggta atattggttc acacgatgat
      781 ttcaaatttc aacgccagtt tcttaatgat cttagggata cgacaattaa agtcagaaaa
      841 gaagaaagtt ttggtaaatt tatgaataaa acggcaaata atcatgctga ttttgcgcga
      901 gcccagcgcg aagcaattat taagaaagta actgaagtat tacgtccgaa atatggccat
      961 atgtatggct acgacgcatc tatgcctgct aatatcgaaa tggcaataag attggtcggt
     1021 tcttattact aagataaaat caacttgcaa gagactgtaa ttatattgca aagtgaaaat
     1081 taacctcggt agcgagatat taccgaggtt aatattggca tacattacac ccttatcagg
     1141 cacttaaacg atacatcata tcttatctgg attattagat gacgagccat acgcaccagg
     1201 taattaagaa tccggtaaaa ccgagaatgg tcgttaacac tgtccaggtt ttcagaccgt
     1261 ctgctaccga caaccccaga tatttggtca caatccagaa ccctgagtca ttaatatgtg
     1321 acgcgccaag cccaccaaag caggctgcca gcgtcaccaa tacgcactga atcggattta
     1381 atcccatcac cgcttctgag agtaacccgc cggttgtcag gattgctacg gttgctgacc
     1441 cctgcgatgc acgcagcgcc agtgaaataa taaatgcggc tggtaacaga ggcaggtcaa
     1501 tcatttgtaa catgttggca agggctttac cgacgcccga ttccaccagc actttgccaa
     1561 atacccctcc agcaccagta accaaaatca ctaccgccgc agtgggaagg gcagagccca
     1621 taatgtcgct ggtgtgttgt aagctccagc cgcgacgtaa agccaataac cagaatgcca
     1681 gcaccagcgc aatcattaga gctaccattg gtgagccgat caactgtagc gtaccaagca
     1741 ggggatgcga aggcggcatc agtgttgcag aaaccgtacc cgccatgata atcgcgatag
     1801 gaataacaat tagcgaggtg accagcgcga cgcccggtgg atttatttta tcgcttaatt
     1861 ttgtcgcgcc ttcctcactg gccggagcca gttgcatctg ttccagtacc tcgaccgaca
     1921 tcgcatattg gcgcttattg attattttcg ctgcaaagta gccaacaatc cctacgggaa
     1981 tagaaatcgc aataccgatg atggttagcc agccgatgtc tgcgtggagt aaccccgctg
     2041 cggcgacagg gcctggatgc ggcggtaccg ccacgtgaac agtgagcatg atcccagcga
     2101 caggcaggcc aaatttgagt ggtgatattt tggcaacctt ggcaaaaccg taaatgattg
     2161 gcgcaagaat aataaagccg acatcaaaga agacgggaat accgaggaag aacgctgcca
     2221 gagtcagcgc agcgatagtt cgtttgtcac ctaacttgcg actgaaataa ttagccagtg
     2281 actctgcacc accagagtgt tcgatcatac gccccagcat agcgcccaga ccaataataa
     2341 tagtgacgga accaagcaca ccgcccatcc cggcgatcat cactttaccc acttcgcccg
     2401 ccggtatacc cgccgcaagt gcgactaaca ggctgacgag gagcagagca acgaatggtt
     2461 gtacctttgc cttgatgacc agtagcaaca gcatgattac gccagctaac gcaatgcata
     2521 acaatgtaat tgtggacatg ggaaaccctg tctgaaagtt atagttaacc taccccatcc
     2581 gtagatgggg ggatgtatgg gtacgttgta attagggatt gagcgaatta gcgccaggcg
     2641 tcaaaccagc caagcccttt ttcggtgagg ccacgaggtt tgtattcaca accgatccag
     2701 ccctgatatc ccacttcatc gaacaggcgg aacagccacg gatagttgat ttctccatcg
     2761 tccggttcat gtcgatcagg tagtccggca atttgtacgt gcgcatattt cccggcgtag
     2821 tcgcggatta aatgcgtcag gttgccatct actttttgcg catggaaagt atccagttga
     2881 ataaacacgt tatctcgcgc aacctcttca acaatagcca gtgcctgata ctggctggag
     2941 aagagataat gaggcttaac gccggggctg agtgcttcaa ccaatattcg cttgccgtgt
     3001 ggcgcaaagc ggtcggcagc gtagcggaga ttatcaataa atactgcctg gtaccgttca
     3061 gcatcttcgc cagcgggcac gacgcctgcc atcacatgga cttgttcaca attgagcgcc
     3121 aatgcatatt ccagtgccag gtcgatgtct gcgcgtgctt cgtgctcacg tccgggaagg
     3181 gcggataatc cccattctcc cgcattaata tctccgggag cggtattgaa cagcgccagt
     3241 gtcagatggt tttgcaccag ttgctgctgg atttgctggg gggagtagtc atagggaaac
     3301 agaaactcca cagcatcgaa cccggctttt cgcgctgcgg cgaagcgttc aataaaaggc
     3361 acttcggtga acatcatgga taaattagct gcaaaacgag gcattgcatt aactccttaa
     3421 ttccgcaatt tcacctgcgg tcagataacg gatcgggcgg tcaccgagaa taaaaatcag
     3481 ctttgccgtt tcctccagct cttccatatt gttggcggct tcttgcaggc tttcaccgca
     3541 aaccactggg ccatgatttg ccagtaaaaa agcctgattg tctgctgcca gttccgccag
     3601 atcctgtgcg atgcgtttat cgcccggtcg gtaataaggc accagcggga cattacccat
     3661 ccgcatcacc acgtatggtg tgaacggccg aataacgttg ctgctgtcca gcccttgcag
     3721 gcaggaaagc gccgtcgacc atgtgctgtg caaatgcacc accgctttac agcgcggatt
     3781 gttgcgatac agcgccagat gaaagagcac ctctttcgag ggtttgtcac cacttaacca
     3841 ttcgccatcc gcggtgactt tggaaagccg ctgcggatcg agattgccca ggcatgaacc
     3901 tgtcggtgtc gccagtaaat tcccgtcagg taaaagcagc gacagattgc cagccgaacc
     3961 agttgcatat ccgcgctgaa agaatgaact ggcaatccgc gtcatctcct ctcgcaaaga
     4021 ctgctctact tttgcgaaat cgctcatgat aaaaactctc tttgggctcg tgaaaaaaag
     4081 gcttcatcac cgaagttgcc agatttaagg gcgagtgaga caggcttatc cagtgcgttt
     4141 acccacggca cgccagggga aatggttggg ccaatatgaa acccttttat tcccaggctc
     4201 tgtgtgacta cgccggaggt ctcaccgcct gcgacaataa agcgtgtcac gccttccgct
     4261 gctaaccgcg ccgctagttg agaaaacagt gtttctactg cctgactggc tttttgtgca
     4321 ccgtattgct gttgaattgc tgccaatgcg tcagtgcttg cggtggcaaa aaccagtgga
     4381 gcaagtacac tttcctggcc cagaacccac tctgccagtt cgtgtgcata agcggccaga
     4441 gtttcggttg agaggcagcg tgccacatca acctcacggg ctggtgcaat ttgacggtaa
     4501 tgtgccacct ggcggttggt catttgagag catgaaccgg agagcactac gccgcgccca
     4561 gcgagcggac gccctgcttc gcgagcctgg ttaccgtttt cttgcgccca ctgccgggcc
     4621 aggccaatgg ccagaccaga accgcccgtt accagtgggg catcgcgcaa ggcttccccc
     4681 tgaatttcca gatggtgttc ggtcagcgca tcaagcaccg cgtagcgata gccctcttgc
     4741 tgtaagcgag ccagctcctg acgaacggct ttcacacctt gttcgaaaac atgtgctgaa
     4801 acgacgccgc agcgccctgt ggattgcgct tcaaccagac ggggaagata gctgtcggtc
     4861 atgggattta ccgggtgatg gcgcattccg gattcggcca gcagttgatt cattacgaac
     4921 aaatacccct gataaaccgt acgtccgttg actggcaggg ccggagagaa gaccgtaaac
     4981 ggcgtgtcga gagcatccat taatgcatcg gtaaccgggc cgatattccc tttcgccgta
     5041 ctgtcgaaag tagagcagta tttgaaatag atctgtttgc aaccttgctg ttgcaaccag
     5101 ctcagagccg ccagcgattg ctgtgtggct tcaaccaccg gacaggagcg cgttttcagg
     5161 ctgatcacca gtgcgtcgat tgcttccggc attttacctg ttggaacacc gttaatttgt
     5221 accgttggta gaccgttttc caccagaaaa ctggcgatat ccgtcgcgcc ggtaaaatca
     5281 tcggcgataa cgccaatctt gatcatgatt tcgctcccgg tagagtgatg ccagagaaaa
     5341 tcttgataac tgcgctatcg tcttctttcc cgtaacccgc gttactggcg ctggtgaaca
     5401 tattcaatgc tgttgaggcc aatggcagcg ggaagtgcag ggctttggcg gtatcggcaa
     5461 ccagaccaag atccttaaca aaaatatcga cggctgaatg cggggtgtaa tcgccatcca
     5521 ccacatgacg catccggttt tcgaacatcc atgaatttcc ggcggcattg gtcacgacgt
     5581 catacatcac atccagcggg atccccgcac gggctgcaag cgccatcgct tcggctccgg
     5641 cagcaatatg tacgcccgct aacaactggt ggataatttt tacggtcgaa cccagtcccg
     5701 gttctgctcc gatgcgataa acttttccgg caacggcttc cagcacgggt gctagtcgtt
     5761 caaaggcaat atcgctaccg gaggccatga cagtcatttc accgttagcg gcttttaccg
     5821 caccaccaga aaccggcgca tccagcattt ccagaccgaa tccagccaga gcggtagcaa
     5881 tttcttgcgc atcagcactg gcgatagtgg aagaaaccat tactgccgta ccgggtttca
     5941 gatgttgtgc aacgcctttt tcaccaaaca gcacctgttt aacctgggtc gcattgacca
     6001 ccagcaccag cagagcgtcc agtttttcgg caaacgtcgc ggcgttatca gaaaccccgc
     6061 aagcaccagc ctctttcaac gtagcgcagg cattgctgtt caggtctgcg ccccaggtag
     6121 aaagacctgc gcggacacat gacagtgctg ctcccattcc cattgaccct aagccaacga
     6181 taccgacatg aaactcagat cccgttttca tctgctctcc ttgttaattt aagtgatatt
     6241 ttgtttgatg ttgtgaatat aagcgcctgg agatatcgat atggtgaggt tgttcacaaa
     6301 aattaacata atgtgttatt ttatgtgaac taagcgatag ttgcgctgcg cacgtttcgc
     6361 aggcaaatag cgtagaatgt ccgcaggaca aaggaaggag caaaagttga tacccgtaga
     6421 gcgtcgccaa atcatccttg agatggtagc tgaaaaaggc attgtcagta ttgctgaact
     6481 aacggacaga atgaatgtgt cacatatgac cattcgtcgg gatttacaaa aactggagca
     6541 gcagggagcc gttgtgctgg tgtccggagg cgtccagtct ccgggacgcg tggcgcatga
     6601 accttctcat caggtaaaaa ctgcgctggc aatgacgcaa aaagcggcta ttggcaagct
     6661 ggcggcaagt cttgttcagc cgggacgttg tatctatctg gatgcgggaa cgaccacgtt
     6721 agcgattgca cagcatctga ctcacatgga gccactcaca gtggttacta acgacttcgt
     6781 tattgcggac tacttgctcg acaacagtaa ttgcacaatt attcacactg gcggcgcagt
     6841 gtgtcgggaa aaccgttcct gtgttgggga agccgctgcg accatgctgc gtagcctgat
     6901 gattgatcag gcttttattt ctgcatcgtc atggagtgtg cgggggatct ctacgccagc
     6961 agaagataaa gtcacggtga aacgggcgat tgccagtgcc agccgccagc gagttttggt
     7021 ctgtgatgcg acgaaatatg gacaggtggc gacatggctg gcgttaccat taagcgagtt
     7081 tgatcagatc attactgacg atggtctgcc ggagagtgcc agccgcgcgc tggcgaagct
     7141 ggatctctct ttgctggtag cgaaaaatga ataatgacct gcaataacgc cgggttactc
     7201 atgcttcaca gaagaagcat gagactactt tattttataa aatgacagcc gcccgctttt
     7261 cggcgatccg gtatctatat aaatctggtt agcgaacgtc tgaatgttat caaacatcat
     7321 atgaccaaat ataaagaagt cggcgccgtt tattttttgt aattcgctat taagtgattt
     7381 ctgcacacga tcaacaggcc agagtaattc gctctccgct atttctttgc caaactgata
     7441 ctccttcccc ggataatctg catgtgcgat gacatatttt atggtgtcgt tagtgatttc
     7501 aataatatgt ggaaggtgat ggaatttcag caacagatct attgcctctt gttgctctga
     7561 atcatttaaa tcgaaaaacc agtcaccacc gctggcaagc cacatatttc catcgccagt
     7621 ttcgaatgca tcaagcgcca tcgcttcgtg gttgcctttg acggagataa accagggctg
     7681 gtttaacagg cgcagcacgt taagactctc gggcccacga tcgatgttat cgcctaccga
     7741 aataagtaag tcggtttcag ggcaaaaaga gagttgatgt aagcgggatt gtaataattg
     7801 atagtcacca tgaatatcac caacggtcca tatatggcga tagtgatggg cgttgatttt
     7861 ttggtagcgt gtagatggca tggttttacc ctgtaaaata agctttccta ttatacaggg
     7921 tatttttatt tgattcgtca gttgttgtta atattcccga tagcaaaaga ctatcgggaa
     7981 ttattattac accagactct tcaagcgata aatccattcc agcgcctgac gcggagtcag
     8041 tgaatccggg tcgaggtttt ccagtgcctc gaccgcaggc gaagtttctt ccggtacgga
     8101 cagcaaagac atttgtgtac catccacttg cgtagcagcg gcgttcggcg aaatgctttc
     8161 cagctcacgc agtttttgcc gtgcgcgctt aataacctct tttggcacac ccgccagagc
     8221 tgcaaccgcc aggccgtagc ttttgctcgc tgcgccatcc tgcacgctat gcataaaggc
     8281 aatggtgtcg ccgtgctcca gtgcatcgag atgcacgttg gcgacgcctt ccattttctc
     8341 cggtaactgg gtcagctcga aatagtgggt agcaaacagc gtcaacgctt tgatcttatt
     8401 tgccagattt tccgcacatg cccacgccag cgacagacca tcgtaagtag acgttccacg
     8461 cccgatctca tccatcaaca ccagactata ttcggtggcg ttatgtaaaa tattggcggt
     8521 ttcggtcatc tccaccataa aggttgaacg cccggaagcc agatcatccg cggcacctac
     8581 gcgggtaaag atacggtcaa tcgggccaat ctcgactttt tgcgccggta cgtagctgcc
     8641 gatatacgcc atcagcgcaa tcaacgcggt ctggcgcata taggtacttt taccgcccat
     8701 gttcggaccg gtaatgatca acatgcgacg ctgcggcgac aggttcagcg ggttagcgat
     8761 aaacggctca ttcagtactt gttcaaccac tggatggcga ccttcggtaa tacgaatgcc
     8821 aggtttatca ataaaggtcg ggcaggtgta gttcagggta taggcccgct ccgccaggtt
     8881 aaccagcaca tcaagttctg ccagcgcgct cgcgctctgt tgcaacgctt ccagatgcgg
     8941 cagcaacagg tcgaacagct cttcataaag ctgtttttcc agtgccagcg ctttgccttt
     9001 tgaggtgaga actttatctt cgtactcttt cagctccgga ataatgtaac gctcggcgtt
     9061 tttcagcgtc tggcggcgca tgtagttgat tggtgccaga tggctttgcc cacggctgat
     9121 ttgaatgtaa tagccgtgca ccgcgttaaa gccaactttc agcgtgtcca ggccggtacg
     9181 ttcacgctcg cggacttcca gacgctccag ataatcggtc gcgccgtcag ccagcgcccg
     9241 ccactcatcc agctcttcgt tatagcccga tgcgataaca ccaccgtcgc gtaccagcac
     9301 cggtggtgtg tcgatgattg ctcgctccag cagatcgcgc agctcggcaa actcgcccat
     9361 cttctcacgc agcgcttgta ccggtgcact atcgacattt tctaactgcg cacgcaactc
     9421 tggcagttgc tggaaagcgt gacgcatacg ggccagatcg cgtgggcgag cggtacgcaa
     9481 cgccagacgc gccagaatac gttccaggtc gccgacctga cgcagtaccg gctgtagctc
     9541 agcggtgaaa tcctgcaatg cgccaatagt ttgctggcgc tcaagcaaca cgcgggtatc
     9601 gcgcactggc atatgcagcc agcgtttcag catacggctg cccatcggcg ttacagtgca
     9661 gtcgagcact gaagccagcg tattttccgc accgccggcc aggttctgag taatttccag
     9721 gttacgacgc gtcgcggcat ccataatgat gctgtcctgc tgacgttcca tagtgataga
     9781 acgaatatgc ggcagggtcg tgcgttgggt atctttcgca tactgcaaca gacaaccggc
     9841 agcacaaagt ccgcgtggtg cgttctccac accaaaaccg accagatctc gggtgccaaa
     9901 ttgcaggttc aattgctggc gagcggtgtc gatttcaaac tcccacagcg ggcgacgacg
     9961 caggccgcgg cgaccttcaa tcagcgacat ctcggcgaaa tcttccgcat acagtaattc
    10021 cgctggattc gtacgttgca gttctgccgc catcgtttcg cggtccgccg gttcgctcag
    10081 gcgaaaacga ccggaactga tatccagcgt tgcgtagccg aaccctttac tgtcctgcca
    10141 gatagccgcc agcaggttgt cctgacgttc ctgcaacagc gcttcatcgc tgatggtgcc
    10201 cggcgtaacg atacggacaa ctttgcgctc aaccggccct ttgctggtcg ccggatcgcc
    10261 aatctgttca caaatagcga cggattcgcc ctgattcacc agtttggcga ggtagttttc
    10321 caccgcatgg tagggaatcc ccgccatcgg gattggctct cccgctgaag caccgcgttt
    10381 ggtcagtgaa atatccagca gttgcgacgc ccgttttgcg tcgtcataaa acagttcata
    10441 aaagtcgccc atccggtaaa acagcaggat ctcgggatgc tgggctttca gcttgagata
    10501 ctgctgcatc atgggcgtat gggcgtcgaa attttctatt gtactcatgg ggttaagtcc
    10561 ggctccctga tgttaaatgt agtgtgatgg tttttatttt cgttgtaatc acataaggcg
    10621 cacatgatac cggagttaat catttcaggg gaagacgcga aaggtgagtt cttgatgtcg
    10681 gttccgcgaa aaatgctgct ttgcagcttt tgcgtgatga cgatgaaaca agatcgccaa
    10741 attcgtcggt tgaagaaacc tcctccttca gagccatagt cccctttaaa attaagttgt
    10801 aatataaatg caccttaaga gtatggaggc agcatgtccg gcaagcgtat ctctcgtgaa
    10861 aaactgacga ttaaaaaaat gatcgatctt tatcaagcga aatgcccgca ggcgtcagca
    10921 gagccggagc attacgaggc gttgtttgct tacgcgcaaa agcggctgga taaatgtgtg
    10981 ttcggtgaag agaaaccagc ctgtaagcag tgtccggtgc actgttacca gcctgcaaag
    11041 cgtgaagaga tgaaacagat tatgcgttgg gccgggccga gaatgctgtg gcgtcatccg
    11101 atcttaaccg tgcgtcatct catcgatgac aagcgtccgg tgccggagtt gccagaaaaa
    11161 tatcgtccaa agaaataaat ctgccggaga gccatactct ccggcaaata aggcgtttac
    11221 gctttggtgg caatcaggat ggctgatgct ttgatcaatg caataacgcg cgagccagtg
    11281 gtgagtttca tttcatcctg actttcattt gttaccactg ccacaatttc aaaacccgca
    11341 tccgttttga tatggacagt ggcgttcact gcgccttcgg tgattgtcga cacgctgcca
    11401 gcaaactggt tgcgagcaga gaatttcagt ccacagtctt ccgttgccag cgtgacccac
    11461 ggggctttaa tcagggcaat cgcttctttc cctttcgcca gacccagcgc ttgctggctg
    11521 ctgtgtgtaa caattgccac cagctttgca ccacctgcca gcgtcagttc tacttcatca
    11581 ttcacagcgc ccattgctac tgcgctaact gttccggtta attgatttct tgctgaaact
    11641 gccatactgc ctcctttcat tgaatgttga tcgagtataa ccacatctgt taaatcaatg
    11701 ccgatttatc aattcccagt cgcttcatcc gtgacagcag agtcgtgcgt ttcagcccca
    11761 gacgttgcgc agcgccttta ggcccggcaa ccacgccgtt tgtttctttc agcacgcgaa
    11821 caatcaactg atattcatct tcgccctcct gggcgacaac cgttgcggca ggcggcgttt
    11881 caggttctgg taaagcaata tctggcaatg acagctgcag cacgttgccg cgtgttagca
    11941 ataccgcgcg ctcaatgacg ttttccagct cacgcacgtt gcccggccat tccatgttgc
    12001 tcaggatgcg cagcgtctcg gcaggaatgc tgtcgatatt gcgccccaga cgacgggcaa
    12061 ttttgaaggt aaaggctttc gccagcagcg gaatatcttc cggacgctcg cgtagtggcg
    12121 gcaggtggat cgggaatacg ttcagacggt aatagagatc gctacggaac tctcggtcgg
    12181 cgaccatttt tttcagatcg cggttagtcg cggcaattaa acgtacgtcc gtctgaatga
    12241 ttttgttgct gccgagacgt tcaaactcct gttcctgtaa tacacgcagc aacttcggct
    12301 gtaactccag cggcatatcg cccacttcat cgaggaatag tgagctttta tccgccagtt
    12361 caaaacgacc aatgcgctga gcgctggcac cggtaaaagc cccacgctca tgaccaaata
    12421 gatcgctttc cagcaatccg gcaggcatcg ccgcgcagtt cattttgacc atacggcgat
    12481 tattacgccc actgagatta tggatcgcgc gggcaatcag ctctttacct gtaccagttt
    12541 caccgaggat cagaacggta ctgtcacttt gcgccaccat ttccacttgt ttaagtacgc
    12601 tgtacatggc ttcgctgcgg ccaataatct cgccaaattc actatcaaca ttgttgagct
    12661 gctcggtcag ggcgaggttt tcatcaacca gccgttcttt cagacgatga atttcctgat
    12721 aggcgagggc gttatcgaca gcgattgcca cacgttcggc aatctggcgc agtaaattca
    12781 gattggtagt ggtaaagact ttctcttcgc attgcgccag ttttagcacg cccagcatag
    12841 tgtcgccaga catcagcggt aacaggcaca aggtttgaat ctggttgccc caggtgttga
    12901 acaacatgcg ttcatagggg gctaaatcgt cccgctcgtg gagattaatc aacagcatct
    12961 ctttactttt gaagacgcgt tcggtgaggg ttccggcttc atcgacttcg ctctgttcgt
    13021 gagcgggatg ctgtttatca agatagtgag tggagtagat gttgagtttg tttttacggt
    13081 ggctgcgtaa gacgatgctg atatcgtcaa tgtcgaagta gtagtggatt tctttggcga
    13141 cttcgctgac cagttcgtcc atatccaggc gggaaagcac cgcgttagtg atggcgacca
    13201 ggatgcggaa gttatcgcgt tcccggcata acaactcata gtcgacattg ttaacgacgc
    13261 ggctctggat ttgttcggtg acgacagaaa cgatctgcgt aaatgtttgc agacgattga
    13321 actctttttc gctccagggg cgatcgtcat aacgaataaa ttcacagcca ccaaaaatat
    13381 gcccttccgc cgccagtggc atcaggcaat agtgaccaaa tttggggtat agcccacctg
    13441 tggccagctg cggccaggtt tcacaaaatt cttcgtaact gcaatgcagc gtatcagggc
    13501 gcgacaaaat gctgcgtacc ggaccatgtg ccagaacagt ttcgtcttca tatttaatgg
    13561 gggtgtcttt ttcacgcgat gcgtaataag acgcacgttg agtctgcgct tgccacaaca
    13621 caatcgccgc gttgtcggcg agcgcagaac gctttaccag ttgcgaaaga gcctcacaca
    13681 gcgaggccag atcgggctgc tgcaatagtg tccgagtgat gtcgaacaac ccttgctgtc
    13741 cgagatcact catcggtgta tatgacattt tgttcttcca ggaaagcacc gcaaagcggt
    13801 gcaaaaggtc attgactaac tataggagat ttagaatttt attagcatat acgcggaagc
    13861 ggttcggcgt gcggtagatc gagggttcgt ttcacgccat acagaccggc aagacgaaca
    13921 cctttacgtt ctactacttc gccaatcagc gccgcatctt ttcccaatgg atgggaatgt
    13981 aacgccgcca gcacttgctc tgctgcgttg cgttcaacgg cgatcaccag tttgccttcg
    14041 ttggcaaagt taagggcgtc cagtcccagc agttcgcaaa cgccgcgcac agcaggttta
    14101 accggcagtg ccgcttctga aagttcaata ccacaaccgc aggctgccgc gaactcatga
    14161 accaccgcgt ttacaccacc acgggtggca tcacgcagcg ctttcacgcc gggaatgtca
    14221 cgcagcgtct gaataagcgg cgtcagcacc gcgcagtcgc tgaccagttc tccatccagc
    14281 cctagttgct cacgcaggtt aaggatagtc gccccgtggt cgccgagtgt accgctaacc
    14341 agcaatacat cgcctgcggt tagcgtctgt gcgccccagt gaatattcgc cggaattgcg
    14401 cccatgccag cggtgttgat aaacagttta tctgccgcgc cgcgctgtac cactttggta
    14461 tcgccagtaa cgatggcaat gcctgccgcg cgggcggttt ctgccatgct ggtcactacg
    14521 gctttcagtg tctccatcgg caatccttct tcgaggataa agccacagga gagatagcgc
    14581 ggaattgcgc cactgaccgc aacgtcattg gcggtgccgc aaatcgccag cttgccgata
    14641 ttaccgccag gaaagaacag cgggtcaatg acgtaactgt cggttgagaa cgccagacgg
    14701 tcgccttccg ctaccagctg tgccaaatca agacgggcct gatcttcctg ctctgccagc
    14761 cacgggttgg caaaggcttc cataaacagg ctgttgatta attgctgcat cgcctggccg
    14821 ccgctaccgt gggcgagttg gatattattc acgcttcact ctcctgctga cgatactgat
    14881 accacgcggc gcacgctcct tcggaggaaa ccatcagcgc accaaacgcg gtttgaggat
    14941 tacaggtgtt accaaacagc gggcattgat gcggcttaca tttgcccgtc aagacttcgc
    15001 cacaacgcgc acgcgggtca tcgcagacct gctgcggtgc cgggcggaaa tgtgcttcgg
    15061 catcgaatcg ttgataatcc ggcgtcaggt gcacgccaga agattcaatc acgcctaagc
    15121 cgcgccattc actgtcgccg ttgacacaga acacatcagc aattgcctgt tgcgccagca
    15181 ggttaccggc atccggtacc acccgacgat actgattctc taccttgctg tgggccgcta
    15241 ttttctgctc caccagcatc accacgcctt gaagtagatc aaggggttcg aaaccagcga
    15301 ccaccagcgg acgatgaaaa tcgctggcga taaaattata ggcatcagtg ccgataacca
    15361 tactgacgtg gcccggcgcg aggaacgcgt cgataccgtt atctggctgt tccagcaaac
    15421 tgcgcagtgt cgggataagc gtaatgtgct ggcagaagaa gtaaaaattc tgcacatcgc
    15481 gggctttcgc ctgttgcaga gtgatggcgg tggtcggcat ggtggtttca aaacctaagc
    15541 cgaagaatac cactttacga gttggattct cctgcgccag tttcaacgca tccattggcg
    15601 agtaaacgat gcgcacatcg gcaccgcgtg cttttgcctg caacagcgac ccctgtttcc
    15661 ctggtacgcg catggcatcg ccaaaggtac agaagatgac ttccggatgg ctggcaatct
    15721 ccacgcaggt gtcgattcta cccatcggca gtacgcacac cgggcaaccc ggaccgtgga
    15781 taaactcaac gttttccggt agcaactggt cgaggccgaa tttaaaaatg gcgtgggtat
    15841 gaccgccaca cacttccata atccgcagag ggcgttcggc ggtgtaagag agatgtgaag
    15901 cacgttcgcg cagatgctca attaactgca ttacctgttc cggcgcgcga tattcatcaa
    15961 caaaacgcat tatttttcct cgccatacaa cagcgcgccg acatctggct caacgtcaaa
    16021 catgttttgt aaggcgtcga gagtgtcgcg tgcttcggct tcattaatta cgctcatggc
    16081 aaagccaacg tgaaccagta cccactggcc cacgcgtggc tgaccgtttt catcgcagct
    16141 gccgactaac gttaaatcga catcgcgctg aatgccgcag acgtcgactt tcgccaggtt
    16201 gccgtcaatg gtgcgtatct ggccgggaac gcctatgcac atcgctgtgt ctccagccag
    16261 ttcagccact ggtccatccc ttcgccgctg gtggcggaaa taaggatgat ttcaatttct
    16321 ggattgactt cacgggcgca ggcgatgcac ttctcaacgt caaagttgag atacggcagc
    16381 aggtcaactt tgttgagcag catcagcgag gcggcggcaa acatatgcgg atatttcagc
    16441 ggtttgtctt caccttcggt aacggaaagc accgccactt tgtgtttttc accgagatcg
    16501 aagctggccg ggcacacgag gttgccgacg ttttcgataa acagaatacc gttatcgtcc
    16561 agtggcaggc gcggcgcggc gtcggcaatc atctgtgcgt caagatggca gcctttaccg
    16621 gtgttcacct gaatcgctgg cgtgccggta gcacgaatgc gcgcggcatc gttcacggtt
    16681 tgctggtcgc cttcaataac tgcgcacgga acgctgtctt tcaggcgcat taaggtttcc
    16741 gtcagcaggg tggttttacc ggaaccaggg ctggaaacca ggttgagcac cagttgcttg
    16801 cgggcagcaa agcgcgcacg gttgcgttca gccagacggt tatttttgtc cagcacgtca
    16861 atttcgactt ccagcatccg acgctggctc atgcccggcg cgtgagtgcc cgcttcgcca
    16921 tgaccgtaat gcaggtcgcc ttcttcagtc tggctggggg taaattcagg cgctttaatg
    16981 ccggtgattt tcatcttcgg gcgtgccgcc ggggcaaatg gcgcgctacg aaacgcggaa
    17041 tgagggttat gttcatcacc ctcgatatag aggttgcctt caccgcaacc gcatgttgta
    17101 cacatcgctc actcctggtc tatttctatc cgccgaatct gtaaaccgtc gtctgccaca
    17161 atctgtagca tgtcaccatg acactgtgga cagcggcgga cgcgctgggt cagtagcgtc
    17221 acatactgtt ggcatgtttc acaccagcat tcggcctctt gttcttcgag gtgcagttta
    17281 caaccttccg ccacgctgcc gcggcaaacc agatcaaaac aaaaggcaag agagctggtt
    17341 tcgacacaag aaaatgcgcc aattttgagc cagaccccag ttacgcgttt tgcgccgtgt
    17401 tttgcggcct gctgttcgat caattccagt gcccgttggc agagggttat ttcgtgcata
    17461 tcgcctccca ttaactattg ccagctacaa gcaataattg tgccagtgtt gattatccct
    17521 gcggtgaata atgtcgatga tgtcgaaatg acacgtcgac acggcgacga aattcatctt
    17581 tagcttataa atcgctttaa taacaatgaa ttaaaagttg gcacagaaaa tgcttaaagc
    17641 tggcatctct gttaaacggg taacctgaca atgactattt gggaaataag cgagaaagcc
    17701 gattacatcg cacagcggca tcgtcgccta caggaccagt ggcacatcta ctgcaattcg
    17761 ctggttcagg ggatcacgtt atcgaaagcg cgcctgcatc acgccatgag ctgcgcgccg
    17821 gacaaagaac tctgtttcgt cctttttgaa cattttcgca tttacgtcac cctggcggat
    17881 ggctttaaca gccacaccat cgagtattac gtcgaaacaa aagatggcga agacaaacag
    17941 cggattgcgc aggcgcagtt gagcattgac ggcatgattg atggcaaggt caacatccgc
    18001 gatcgcgaac aagttctgga acactatctc gaaaaaatcg ctggcgttta cgacagctta
    18061 tataccgcca ttgaaaacaa tgtgccggtg aatttaagcc aactggtaaa gggacaaagc
    18121 ccggtagcat gagctgaggc tttgcccgtt ttgcaggcgt tacgcctgtt tggggatggg
    18181 cgtgtcgatg agtgtcgaaa atgacatttc atcggcatgt tttcgtcaaa aatgacaatc
    18241 acctgaggaa tgcctggtga atcgttttgt aattgctgac tccacgctct gtatcggctg
    18301 ccacacttgt gaggctgcct gttcagagac gcatcgccag cacggcctgc aatcaatgcc
    18361 gcgcctgcga gtgatgctca atgaaaaaga atctgcgccg cagctctgtc accactgtga
    18421 agatgcaccc tgcgcaacgg tctgcccggt taacgccatc actcgtgttg atggtgccgt
    18481 gcagctgaat gaaagcttgt gcgtaagctg caaactgtgt ggcatcgcct gcccgtttgg
    18541 cgcaattgaa ttttccggca gccgtccgct ggatattccg gcaaacgcca ataccccgaa
    18601 agcgccaccg gcaccgcctg ctccggcgcg tgtcagcaca ttgcttgact gggtgccagg
    18661 tattcgcgcg atcgccgtca aatgtgacct ttgtagcttt gatgaacaag gtccggcctg
    18721 cgtgcggatg tgcccgacta aagccctgca tctggtggat aacaccgata tcgcccgcgt
    18781 cagcaaacgt aagcgtgagc tgacttttaa cacggacttt ggcgatctca ccttgtttca
    18841 gcaggctcaa agtggagagg ctaaatgagc gcaatttccc tgatcaatag cggcgtggcg
    18901 tggtttgtcg ccgccgctgt tctggcattt ctcttttctt ttcaaaaggc gctaagcggc
    18961 tggatagccg gaattggcgg cgcggtgggt agcctgtata cggcagccgc gggcttcact
    19021 gtgctgactg gcgcggttgg cgtgagcggt gcgctgtcgc tggtaagcta cgatgtgcaa
    19081 atctctccgc ttaatgcgat ttggctgatt acactcggcc tatgcggtct gtttgtcagc
    19141 ctctacaaca ttgactggca tcgccacgcg caggtgaaat gcaacggctt gcagatcaat
    19201 atgttgatgg ctgccgccgt ctgtgccgtc atcgccagca acctcggcat gttcgtggta
    19261 atggccgaaa tcatggccct gtgtgcggtg ttcctcacca gcaacagcaa agagggcaaa
    19321 ctgtggtttg cgctggggcg tcttggcact ctgctgctgg cgattgcctg ctggctgctg
    19381 tggcagcgtt acggtacgct ggatctgcgc ctgctggata tgcgtatgca acagctgccg
    19441 ctcggttccg atatctggct gctcggtgtg attggctttg gcctgctggc cgggattatt
    19501 ccgctgcacg gctgggtgcc acaggcacat gctaacgcct ctgcgccagc tgccgcgctg
    19561 ttttccacgg tagtcatgaa aattggcctg ctgggcattt taactctgtc actgctgggc
    19621 ggtaatgcac cgctgtggtg ggggatcgca ctgctggtac tcggcatgat caccgcattc
    19681 gtcggcggtc tgtatgcgct gatggaacat aatatccaac gtctgctggc ttaccacacc
    19741 ctggagaata tcggcattat cctgctgggg ctgggcgctg gcgtaacggg tatcgcgctc
    19801 gaacaaccgg cgctgattgc ccttggtctg gttggtggtc tgtaccatct gcttaaccat
    19861 agcctgttca agagcgtact gttccttggt gcggggagcg tctggttccg taccggtcat
    19921 cgcgatatcg aaaaactcgg tggtattggc aagaaaatgc cggttatctc catcgccatg
    19981 ttagtcgggc tgatggcaat ggctgcgctg ccgccgctga acggttttgc cggggaatgg
    20041 gttatctatc aatccttctt caaactgagc aatagtggcg cgtttgttgc ccgtcttctc
    20101 gggccgctgc tcgccgtggg gctggcaatt accggtgcgc tggcggtgat gtgtatggcg
    20161 aaagtttatg gcgttacttt cctcggcgcg ccgcgtacca aagaggccga aaacgccacc
    20221 tgtgcgccgc tcctgatgaa cgtaagcgta gtggcactgg cgatttgctg cgtaattggc
    20281 ggtgttgctg cgccgtggct actgccgatg ctctctgctg ctgtacctct gccgctggag
    20341 cctgctaaca ccaccgtttc tcaaccaatg atcacgttgc tgctgattgc ctgcccgctg
    20401 ctgccattca tcattatggc gatttgcaaa ggcgatcgtt tgccgtcgcg ttcccgcggc
    20461 gcggcctggg tgtgcggcta cgaccatgaa aaatcaatgg tgattaccgc tcacggtttt
    20521 gccatgccgg tgaaacaggc gtttgcgccg gtgctgaaac tacgcaaatg gctgaatccg
    20581 gtgtcgctgg tgccgggctg gcagtgtgag gggagtgcgt tgctgttccg ccggatggcg
    20641 ctggttgaac tggcggtgct ggtggtgatt attgtttcac gaggagcctg agaatgagtg
    20701 ttttatatcc gttaattcag gcgctggtgc tatttgccgt tgcgccgctg ctctccggta
    20761 ttacccgcgt agcgcgcgcc cgtctgcata accgtcgtgg gccgggtgtg ttgcaggagt
    20821 atcgtgacat tatcaaactg ctggggcgtc agagcgttgg cccggatgcc tccggctggg
    20881 tgttccgcct gacgccgtat gtgatggtgg gcgtcatgct gactatcgcc actgcgctgc
    20941 cggtggtgac cgtcggctct ccgctgccgc aactgggcga tttgatcacc ttactgtatc
    21001 tcttcgccat cgcgcgtttc ttctttgcca tttctggtct ggatactggt agcccgttta
    21061 ccgctatcgg cgcgagccgt gaagcgatgc ttggcgtgtt ggttgaaccg atgctgctgc
    21121 ttggtctgtg ggtcgccgca caggttgccg gttccaccaa catcagcaac atcaccgaca
    21181 ccgtttatca ctggccactg agccagagca tcccgctggt gctggcgctt tgtgcctgtg
    21241 cgttcgccac ttttatcgaa atgggcaaac tgccgttcga cctggcggaa gccgagcagg
    21301 agttgcagga aggtccgctc tctgagtata gcggcagcgg ctttggcgtc atgaaatggg
    21361 gcatcagcct gaaacagctg gtggtgttgc agatgttcgt cggggtgttt attccgtggg
    21421 gacaaatgga aaccttcacc gtcggtgggc tgctgctggc gctggtgatt gccatcgtca
    21481 aactggtggt tggcgtcctg gttatcgcgc tgttcgaaaa cagcatggcc cgtctgcgtc
    21541 ttgatattac tccgcgcatt acctgggctg ggtttggctt tgcattttta gcgttcgtct
    21601 ccttgctggc ggcgtgatta aagagagttt gagcatgtct gaagaaaaat taggtcaaca
    21661 ttatctcgcc gcgctgaatg aggcatttcc tggcgtcgtg ctggaccacg cctggcagac
    21721 caaagatcag ctaaccgtca ccgtgaaggt gaactacctg ccggaagtgg tggagtttct
    21781 ctactacaaa caagggggtt ggctgtcggt gctgtttggt aacgacgaac gcaaactgaa
    21841 tggtcattac gccgtttatt acgtgctgtc gatggagaag ggcaccaagt gttgggtaac
    21901 ggttcgcgtc gaagttgacg ccaacaaacc ggagtatcct tccgtgacgc cgcgcgttcc
    21961 ggcggcggtg tggggcgagc gtgaagtgcg cgatatgtac ggtttgattc cggttggtct
    22021 gccggatgaa cgtcgtctgg tgctgccgga tgactggccc gatgaacttt atcctctgcg
    22081 taaagacagc atggattatc gtcagcgtcc ggcaccgact accgatgctg aaacctacga
    22141 gttcatcaac gaactgggcg acaagaaaaa caacgtcgtg ccgattggtc ccctgcacgt
    22201 cacttctgac gaaccgggtc acttccgtct gttcgtcgat ggtgaaaaca ttatcgacgc
    22261 cgactaccgc ctgttctacg tccatcgcgg catggaaaaa ctggcggaaa cccgtatggg
    22321 ttacaacgaa gtgaccttcc tctctgaccg tgtgtgcggg atctgcggct ttgctcacag
    22381 caccgcctat accacgtcgg tggaaaacgc gatgggtatt caggtgccag aacgcgcgca
    22441 gatgatccgc gccattctgc tggaggtaga acgcctgcac tcgcatctgc tcaatctcgg
    22501 cctggcctgt cactttaccg gcttcgactc cggctttatg cagttcttcc gcgtgcgtga
    22561 aacctccatg aaaatggcag agatccttac cggtgcacgt aaaacctacg gcctgaacct
    22621 gatcggcggg attcgtcgcg atctgctgaa agacgacatg atccagaccc gccagctggc
    22681 acaacagatg cgtcgtgaag tgcaggagct ggtggatgtg ctgctgagca caccgaacat
    22741 ggaacaacgc accgtcggta ttggtcgtct ggacccggaa attgctcgcg acttcagtaa
    22801 cgttggcccg atggttcgcg ccagcggtca cgcccgcgat actcgcgccg atcacccgtt
    22861 tgtcggttat ggcctgctgc caatggaagt ccacagcgag cagggctgcg acgttatttc
    22921 ccgtctgaaa gtgcgaatca acgaggtcta taccgcgctg aacatgatcg actacggtct
    22981 ggataacctg ccgggcggtc cgctgatggt ggaaggcttt acctacattc cgcaccgttt
    23041 cgcgctgggc tttgccgaag cgccgcgcgg cgacgatatc cactggagca tgaccggcga
    23101 caaccagaag ctgtaccgct ggcgctgccg tgcggcgacc tacgcaaact ggccgaccct
    23161 gcgctacatg ctgcgcggca acaccgtttc cgatgcgccg ctgattatcg gtagcctgga
    23221 cccttgctac tcctgtaccg accgcatgac cgtggtcgat gtacgtaaga agaagagcaa
    23281 agtggtgccg tacaaagaac tcgagcgcta cagcattgag cgtaaaaact cgccgctgaa
    23341 ataaggaatc gccatgttta cctttatcaa aaaagtcatc aaaaccggca cggcgacttc
    23401 gtcttatccg ctggagccga ttgcggttga taaaaacttc cgtggtaagc cagagcagaa
    23461 cccgcagcag tgcatcggct gcgcagcctg cgtcaatgcc tgcccgtcaa acgccttaac
    23521 ggttgaaact gacctcgcca ccggtgagct ggcgtgggag ttcaatcttg gacgctgcat
    23581 cttctgtgga cgctgcgaag aggtctgccc gacggcggcg attaagctgt cgcaagaata
    23641 cgaactggcg gtgtggaaga aagaagactt cctgcaacag tcccgcttcg cgctgtgcaa
    23701 ctgccgcgtc tgcaatcgtc ctttcgccgt ccagaaagag atcgactacg tcattgcgct
    23761 gcttaagcac aacggcgaca gccgcgcgga aaaccaccgc gaaagctttg agacttgccc
    23821 ggaatgtaag cgccagaaat gcctggtgcc gtccgaccgt attgaactga ctcgccatat
    23881 gaaagaggcc atctgatgag caatttatta ggcccccgtg acgccaacgg cattccggtc
    23941 ccgatgacgg tggatgaatc catcgccagc atgaaggcgt cgttactgaa aaaaatcaaa
    24001 cgttctgcct acgtttatcg cgtggactgc ggcggctgta acggttgcga aatcgaaatt
    24061 ttcgccacgc tttcgccgct gttcgatgca gaacgcttcg gcattaaagt cgtcccgtca
    24121 ccgcgtcatg cggatatttt actgtttacc ggcgcggtca cccgtgcaat gcgatcccct
    24181 gcgctgcgtg cgtggcagtc cgcgccggac ccgaaaatct gtatctctta cggtgcctgc
    24241 ggtaacagcg gcgggatctt ccacgatctc tactgcgtgt ggggcggtac ggataaaatc
    24301 gttccagtgg atgtttatat ccctggctgc ccgccaacgc ctgccgccac gctgtacggc
    24361 tttgcgatgg cgctcggcct gctggagcag aaaatccacg cccgtgggcc gggtgagcag
    24421 gatgaacaac cggcggagat tctgcatggc gatatggtgc agccgctgcg cgtgaaagtg
    24481 gatcgcgaag cacgccgcct ggcgggttat cgttacggtc gccagattgc cgatgatttc
    24541 ctgacgcaat tagggcaggg cgaagaacag gttgcacgct ggctggaagc ggaaaacgat
    24601 ccgcgtctga acgagattgt cagccatctg aatcatgttg ttgaagaggc gcgtatccga
    24661 tgagtgaaaa ggtggtgttc agtcaactga gccgtaaatt cattgatgag aacgatgcta
    24721 cgcccgccga ggcgcagcag gtggtctatt acagcctggc gattggtcac caccttgggg
    24781 ttatcgattg cctggaagcg gcgctcacct gcccgtggga tgaatatctg gcatggattg
    24841 ccactctgga ggcgggcagc gaagcccgcc gtaaaatgga aggcgtgccg aaatatggtg
    24901 agatcgtcat cgacattaac catgtgccga tgctggccaa cgcattcgat aaagcccggg
    24961 cagcgcaaac ttcgcagcag aaggaatgga gtacaatgct gttaagtatg ctgcatgata
    25021 ttcatcagga aaacgccatc tatttgatgg tgaggagact gcgtgactga cgttttactc
    25081 tgtgttggca atagcatgat gggcgatgat ggcgcaggcc cgctactggc ggaaaagtgc
    25141 gctgccgcgc cgaaaggtaa ctgggtagtg attgacggcg gtagcgcacc ggaaaacgac
    25201 attgtcgcta tccgcgaact gcgcccgaca cggctgctta ttgtcgacgc cacggatatg
    25261 gggctaaacc ctggcgagat ccgcatcatc gacccggctg acatcgccga gatgtttatg
    25321 atgactaccc ataacatgcc gctgaactat cttatcgacc agttgaaaga agatattggc
    25381 gaagtgattt tccttggcat ccagccggat atcgtcggct tttactaccc gatgactcag
    25441 cagattaaag atgcggtgga aacggtgtat cagcggctgg aaggctggga aggaaatggc
    25501 ggcttcgcgc agttagcggc ggaagaataa ttcttcatta gggaattcag ggaattgaac
    25561 atcaaaacag ggatgtgcgc ttttttattg agcttagtgc tgcctgcaca ggccaccagc
    25621 tttaccgaat atctgccaat gagtgacagc gaatacgcgc aaaagcgggc gctgaaacct
    25681 ctcctgacca tgccttatga tgccgatcaa aactggcatt ttagaaaagt gggcgttgca
    25741 ggcgtaacgc ttgagaagat gccaaatgaa gatgattact ggcgattaac cgctaaggac
    25801 cgtgccggta aatcctggtt tgtgcctgtg ggtgtgctgc aaaatatggc gggtaatgcg
    25861 caattttacc gtgctgacct cgatcgtaat ggcattcagg atttggttat ctggcgaggt
    25921 gtttcgggaa acgggctggc gccttcctcg tttttgatcc tgatgacctt taatcagcaa
    25981 ggtcgcccgt gtgttttcca ggtcgatggt ttttacaccg caaacgaaac tggcgttgat
    26041 gatttgcttg atttacaagg caatggtcac acgcaattgc tggatatgca gtttgattcc
    26101 ggttactgga taaccagttt gtatcaggta aaagaggcca aatggcagcg cgttcacggc
    26161 tggtttggca agttaagcta ccccgcactc acgcgtttta cctatacctc caaccgtaaa
    26221 ctcgtcatta aacctgtcgc cggacgtaac ccgcaaacga aagatctggt gctgacacag
    26281 cgttgtttaa taaagggtga tgtgcttgat ggggtgaatc agaactgact ttttgcagag
    26341 tggctatgag gatttttgta gtaattatat cgtgcgcaag tgtttacata ttgtgaactt
    26401 ggatttgata agctatagtg ttccgaacat ataaggatag aactcgcaga tgtggctgtt
    26461 tacttactat aaggaagtta aaacttaaaa atgcacatca tttctaaagc gccatttgaa
    26521 gagtctgcgc gtaaatatcc taacgacgct ctggctcttc aggcattgta tcgggttatt
    26581 aaggaaacgg atttttctac gccagaagaa atgcgaacgg cgtttcccaa cctcgacaat
    26641 tttaaatatc gcaacaaatg gtacgttctg gatgtaggtg ggaacaacct aagggtgatt
    26701 gcctacataa actttgttaa taagcggttc tttgtgaagc acataactaa tcacgccgag
    26761 tatgacaaac tgacacgtta ctacagagag aacaaagaat gactgctaat gctgtacgcg
    26821 ctgttaaagc aacaagagaa ctggtcaatg cagtcccctt tctgggaggc agtgactcag
    26881 aagatgatta tcgtgaagca ctggaactgg tcgaatattt gattgaagaa gatgacacca
    26941 atccacttat cgactttctg acgagccgca ttgcagaata tgaaaacaac aacgaaaagt
    27001 ttgcagagtt cgacaaagcc gttgcggcaa tgcctgttgg tgtggcgtta cttcgcacgc
    27061 tgattgatca acataatctg acctatgcgg atttgaagaa cgaaatcggc tctaaatccc
    27121 tggtcagcca gatcttgtct ggacaacgat ccttgacgat atcccacatt aaggcacttt
    27181 cagcccgatt tggcgttaag ccggaatggt ttctttgact gatggcagac gctaaatcgc
    27241 ctgatgcgct acgcctgtcg ggctttgaaa gggcgcagat tcttaatttt gcgcgccctt
    27301 gtttgtcatt caggctgttg tctttactac tcttaattta tcagtaatct caatcagata
    27361 tgcttttgcc ttttcgcgtg ctcgcccctc aaaaagcatt atccactcat gtgtgtcgtc
    27421 gattgtttgt aaacggccct ggagtttttt gctgaaaatc gacgtgagcc gataattgtc
    27481 gagttgatca atttgtttac tcgatgtgat tttttccccg gctgcttttt gaatgataag
    27541 ttcgacgaga ccgataacat tattatcgta gagaaattct tcatctccct gaaatccgaa
    27601 caaatcttca tgctggctta agcgttgaac aattgactcg gcatcaaagg tcgaatcatc
    27661 ttgcagttgt tcctctaccc aattaatcaa atccagccct tcgtcgcttt cgagagcctc
    27721 cattccccat gaacccattt tgtatcctta cctgttattt cagtcggaat tgtattattt
    27781 aataaaggtt catcgctaat ctgcgatgaa cctcaaaata caatcatatg gatagctgac
    27841 aaagtggtca ttgttcacgc cgtatccggc acggttgctt cccttaaatc ctccccgtta
    27901 ctggcaatta cttttttata ccaccagaac gacttgtcgg aaaaaagtac ccagatgtgg
    27961 actggcaacg ataaatactg tcatcacatg tcatcgtcac tagtgtcgat gacatcgttt
    28021 ttcctgtagt acagaattta atttccttaa ataacagtaa attaaaaact ggcatgattt
    28081 gtgaatgtat cggcgcatta actgttattg ctggagaatt tgatgaaccg tttcatcatt
    28141 gctgacgcga gtaaatgtat tggttgccgt acctgtgaag tagcgtgcgt ggtttctcat
    28201 caggaaaatc aggactgtgc atcgctgacc ccggaaacat ttttaccgcg tatccatgtc
    28261 attaaaggtg tgaacatttc cacggcgaca gtttgccgtc agtgtgaaga tgcaccgtgc
    28321 gctaacgtct gcccgaatgg tgctatcagc cgtgataaag ggtttgttca tgtcatgcag
    28381 gaacgttgta ttggttgcaa aacctgcgtt gtggcttgcc cgtatggtgc gatggaagtg
    28441 gtggtgcgtc cggtgattcg caacagcggc gcagggctga atgtacgggc tgacaaagcc
    28501 gaagccaata aatgcgacct gtgcaaccat cgtgaagacg gcccggcgtg tatggcggct
    28561 tgcccgaccc atgcgctgat ttgtgtcgat cgtaataaac ttgaacaact gagtgcagaa
    28621 aaacgccgcc gcacggcgct gatgttctaa aaatcttaca gaactgttct ggtatcaaag
    28681 gaattcccgg aaatattcgt agaatacgaa gcgggcagta tcgccgacct gtgactgccg
    28741 gatgcgacgc tgtcgtgtcc ggctgggcgg gttacgcaat tcatgatggc gggaataact
    28801 caatggcaaa aaacacatct tgcggtgtcc aactgcgtat tcgtggcaaa gtgcagggcg
    28861 tcggttttcg tccgtttgtc tggcaactgg cacagcaatt aaatcttcac ggcgatgtct
    28921 gtaatgacgg cgatggcgta gaagtccggc tgctggaaga tccggaaacg tttcttgttc
    28981 aattgcatca gcactgcccg ccactggcgc gtattgatag cgtcgagcgt gagccgttta
    29041 tctggtcaca actgcccact gagttcacca tccgccagag cgcgggtggc gtcatgaata
    29101 cgcaaattgt tcccgatgcc gcgacttgcc ctgcttgtct tgccgaaatg aataccccag
    29161 gcgaacgacg ctggcgttat ccgtttatca actgtactca ctgtggtccg cgtttcacca
    29221 ttattcgcgc catgccttac gaccgcccgt ttaccgtgat ggcggcgttt ccgctgtgtc
    29281 cggcctgtga caaagagtac cgtgacccgc tcgatcgtcg cttccacgcc cagccggtgg
    29341 cctgcccgga gtgtggcccc catcttgaat gggtaagtca tggtgaacat gcagaacaag
    29401 aggcggcatt acaggcagct atcgcacagt taaaaatggg caacattgtc gccatcaaag
    29461 ggattggcgg atttcatctt gcctgcgatg cacgtaacag taacgcggtg gcgacactgc
    29521 gggcgcgcaa acatcgcccg gcgaaaccgc tggcggtcat gttgccagtg gctgacggtt
    29581 taccagacgc tgcgcgccag ttgcttacca cgcccgccgc gccgattgtg ctggtggata
    29641 aaaaatacat acctgagctt tgtgatgata tcgcccctgg ccttaacgaa gtcggggtaa
    29701 tgttgcctgc gaacccgctc cagcatttgc tgttacagga actgcaatgc ccgctggtga
    29761 tgacctccgg caacctgagc ggtaaaccac cggctatcag caacgaacag gcgctggcgg
    29821 atttgcaggg cattgccgac ggattcttga tacataaccg cgacatcgtg cagcggatgg
    29881 atgattcggt ggtgcgcgga agcggcgaaa tgctgcgccg ttcgcggggg tatgtgccgg
    29941 atgcgctggc tttgcctccg ggctttaaaa atgttccgcc tgtgctgtgt ctcggcgcgg
    30001 atctgaaaaa taccttctgc ctggtgcgcg gcgaacaagc ggtgttgagt cagcatctgg
    30061 gcgatttaag tgacgatggc atccagatgc agtggcgcga agcgttacgc ctgatgcaaa
    30121 acatctacga tttcactccg caatacgttg tgcatgacgc gcatccgggc tatgtctcca
    30181 gccagtgggc gcgtgagatg aatctgccga cgcaaacggt gctgcatcat catgcccacg
    30241 cagcggcgtg tctggcagaa cacctctggc ctctggatgg cggagatgtc attgcgttga
    30301 cgctcgacgg tattggtatg ggggagaacg gcgctttgtg gggcggcgag tgcttgcggg
    30361 tgaactatcg cgaatgccag cacctgggcg gcttgcccgc agtggcgctt ccggggggcg
    30421 atttggctgc gaagcagccg tggcgaaacc tgctggcgca gtgcctgcgc tttgtgccgg
    30481 agtggcagaa ttactctgaa acggcaagtg tgcaacagca aaactggagc gtgctggcgc
    30541 gggctattga gcgtggaatt aacgcgccgc tggcatcgtc gtgtggacgt ttgttcgatg
    30601 ccgtagcggc ggcgctgggc tgtgcgccag ccacgttaag ttatgaaggt gaagcggctt
    30661 gtgcgctgga ggcgctggcg gcttcgtgcc acggcgtgac gcatccggtg acgatgccgc
    30721 tggtggacaa tcaactggat ctcgccactt tctggcagca gtggctgaac tggcaggctc
    30781 cggttaatca acgcgcgtgg gcgtttcatg atgcgctggc gcagggtttt gccgcgttga
    30841 tgcgtgagca ggccacgatg cgtggtatca ctacgctggt atttagcggc ggagttattc
    30901 ataaccgttt gctgcgtgca cgtctggcgc attatctcgc tgatttcaca ttgctctttc
    30961 cacagagttt acctgcgggc gatggcgggt tgtctctggg gcagggggtt attgttgcgg
    31021 cgcgttggtt ggcaggggaa gtccagaacg gataaaacgt gctgcatttg gacaccattg
    31081 ccggatgcgg cgtgaacgcc ttatccggcc tacatattca tccccgcagg tcggataaga
    31141 cgcgccagcg tcgcatccga cattttggga cagtgcagga tgcgacataa acgtattatg
    31201 tgatcaacac atgcgttccc tgtaggtcgg ataagacgcg tcagcgccgc atccgacatt
    31261 tggggcactg tagctcgcct acatcgacaa cgttttcaac aatccaaatg cctctttcat
    31321 ccgatcctca ctgaccacaa aggcgcgaag ctggtcagca tcgtcaacgc cgcgcgccac
    31381 cattccctgg cgttcggtat taatttgcca gcgtaaatcc tgacgctggg tttcgcctgc
    31441 cagatgcagc ggtaattccg gcgttttgat tttcaccagc atcgccggga gtttcagcgg
    31501 cgtgttattg ccgagaagat tttttgccag caccatcgcg ctaagttgaa tcggctggag
    31561 gaacggcaat acctgaccgt taatttccgc gcaatcgccc agcgcataaa tatcggcatt
    31621 actggtttgc agataactat cgacgcaaac gccgcgatta atcgtcagcc cggcgcgtcg
    31681 tgccagggcg gtttccgggc gcagtccggt ggcggcaatt accgcatcca cttcgatgca
    31741 gcgctggcgg tccagcgttg ccagaatgcc agaatctgtt ttttctagtc cctgcaactg
    31801 agatttcaac agcagatgaa cgcccatctc cgtcaaccga tgctgcaagc ggctgctgac
    31861 ttccggtggc attaacgacg ccagaatact ggcagcgttg tcgattagcg tgaccatttt
    31921 gcctgcccga caaaaatcca tcgccagttc gctaccaatc aaaccaccgc cgataatcaa
    31981 cacgcgtcgg gcatcccgca gttgcgtttc acaggcgcga tactcttgct gactatttaa
    32041 cgtcagcatt aactcacgcc cgggcactgg cgggacaaag gcgctggcac cggttgccag
    32101 cactaacttg tcgtattgcc actgattatt ctgacttttc accacatggg cttcggcatc
    32161 gatatccgtt acccaggtgt gcggaaacag gcgcagatta aactgctcgg caaattcacc
    32221 tgccgtctgg cgggtaaggt cgtcggcacg ttgcccctga ctgataacat ggctgaggtc
    32281 aggtttgttg tactcatcca tgctgtcggc ggcaatcaga gttaatggaa tactggcgtc
    32341 ctgtttgcga atatttttca ccagttggcg ggcggcgaag cccgaaccaa tgatcacaat
    32401 gccgttgctc attttgcctc cgatgccagt tcgtcaaaga cgtctttgcc gagagagcat
    32461 tccgggcaca ggaagttatc cgggacttca ctccacggcg ttcctggcgc aacgtcctgc
    32521 attggctcgc cttttgccgg atcgtaaatc cactggcaga cgctgcattg catccgtggg
    32581 ccgaggtcag ccgtcgtggc ggcagaggtt tcttcttcaa ccaccgtatt caccgtgctc
    32641 tgtggcagcg gcgcgagcgc ccactgacgg gcgatttcgc gaccgtgttc acgacataac
    32701 tccagagcgt cctggtctgg tcgccatttg gctttcaggc taagcgacat ttcgaaaccc
    32761 gcatcctgca ggcgcgtgga aagacgatcc accgcaccgc cgctccagcc gtgagagccg
    32821 aaagcgctgg cgcgtttgtt acggaagcgt aatccggtca tctcctccac cagcccggcg
    32881 attttcggca tcatcacatt attcatcgta gaagtgccga ccagtacgcc ttttgagcgg
    32941 aagacattgg tcaggatttc gtttttatcg cttcgggcga cgttgaaaat tttcaccgcc
    33001 acgcgtgggt cggtttccgc aatcccctgg gcgatagcgt cagccatcat gcgggtgtta
    33061 ttcgacatgg tgtcgtagac aatggtgatt ctgtcttcct gataatccgc cgcccatttc
    33121 aggtacagct cgacaatttg cgtcgggtta tcgcgccata ccacgccgtg agaggtcgct
    33181 atcatatcga ctggcaagtt aaagcccagg atctcggtaa ttttcggcgt taccaggcgg
    33241 ctgaacggcg tcaggatatt ggcgtagtaa cgctggcact gctcgaaaag ctcagtctga
    33301 tccacttcat cgttgaacag atgctcgtcg cagtagtgct gaccgaaagc atcgttactg
    33361 aacagcaccg cgtcgcctgt caggtaagtc atcatgctgt ccggccagtg cagcattggc
    33421 gtttcgacaa aaatgagttg tttgccgttg ccgatatcca gcgtgtcgcc agttttcact
    33481 acattaaaat tccactccgg atgatggtga tgaccattta tcgagtcgat agcgttggca
    33541 gtacagtaga tcggcgtatc gggaatttgt gccatcagtt cggtcagcgc cccggcgtgg
    33601 tcctcttcag catggttaat tacgatgtaa tcgatatccg ccagatcgat ttcattacgc
    33661 aggttctgca caaattcgcg gctgaattta tggtcgacgg tgtcgatcag cacgtttttt
    33721 tcttcgcgga tgaggtagct gttgtagctg ctgccgcgca gcgttttata ttctgtaccg
    33781 tgaaaatcac gcacttccca gtcacgttga ccaacccaat gaatgttatt tttcaccaca
    33841 atagacatag caacctcaat ttattcagcg tgttctaaaa agatgtcttg ctaattgcag
    33901 attgcgtgcc agttttttat ctaattgttt ttactctata aataaaattg tgctcattaa
    33961 tgagtagtca aaatgactat caataaaatg gtcgatatga caatatctat agtcaaattg
    34021 acagtgaggc aaagatgagt ttttccgttg atgtgctggc gaatatcgcc atcgaattgc
    34081 agcgtgggat tggtcatcag gatcgttttc agcgcctgat caccacgctg cgtcaggtgc
    34141 tggagtgcga tgcgtctgcg ttgctacgtt acgactcgcg gcagtttatt ccgcttgcca
    34201 tcgacgggct ggcgaaggat gtactcggta gacgctttgc gctggaaggt catccacggc
    34261 tggaagcgat tgcccgcgcc ggggacgtgg tgcgctttcc cgcagacagc gaattgcccg
    34321 atccttatga cggtctgatt ccagggcaag agagtctgaa ggttcacgcc tgcgttggtt
    34381 tgccgttgtt tgccgggcaa aacctgatcg gtgccttgac gctcgacggg atgcagcccg
    34441 atcagttcga tgttttcagc gacgaagagt tacgcctgat tgctgcgctg gcggcgggag
    34501 cgttaagcaa tgcgttactg attgaacaac tggaaagcca gaatatgctg ccaggcgaag
    34561 ccgcgccgtt tgaagcggtg aaacagacgc agatgatcgg tctgtcgcca ggcatgacgc
    34621 aattgaaaaa agagattgag attgtggcgg catccgatct caacgtttta attagcggtg
    34681 agacgggaac cggtaaggag ctggtggcga aagcgattca tgaagcctcg ccacgggcgg
    34741 tgaatccgct ggtctatctc aactgtgccg cactgccgga aagtgtggcg gaaagtgagt
    34801 tgttcgggca tgtgaaaggg gcgtttactg gtgcaatcag taaccgcagc gggaagtttg
    34861 aaatggcgga taacggtacg ctgtttctcg atgagatcgg cgagttgtcg ttggcattgc
    34921 aggccaagct gctgagggtg ttgcagtatg gcgatattca acgcgttggc gatgaccgca
    34981 gtttgcgggt cgatgtgcgc gtgctggcgg cgactaatcg tgacttacgc gaagaggtac
    35041 tggcagggcg atttcgcgct gacttgttcc accgcctgag cgtgtttcca ctttcagtgc
    35101 cgccgctgcg tgagcggggc gatgatgtca ttctgttggc ggggtatttc tgcgagcagt
    35161 gtcgtttgcg gctggggctc tcccgcgtgg tattaagtgc cggagcgcga aatttactgc
    35221 aacactacaa ttttccgggg aacgttcgtg aactggaaca tgctattcat cgggcggtag
    35281 tgctggcgag agcaacccgc agcggcgatg aagtgattct tgaggcgcaa cattttgctt
    35341 ttcctgaggt gacgttgccg ccgccagaag tggcggcggt gcccgttgtt aagcaaaacc
    35401 tgcgtgaagc gacagaagcg ttccagcgtg aaaccattcg tcaggcactg gcgcaaaatc
    35461 atcacaactg ggccgcctgt gcgcggatgc tggaaaccga cgtcgccaac ctgcatcggc
    35521 tggcgaaacg tctggggctg aaggattaaa taatcccggc ctgatagaaa tcctgcaggt
    35581 taattgcgcc ggtgagtttg ccgttttcat ccactaccgg tgcggcggtg attttgcgtt
    35641 tcatcaggat ttctttggcg tcgatggcac ggctttgcga ttgcaacgtg gtgccgccga
    35701 ccgtcatcgc ttcattgact ggcgtggtga gtgcgccgcc gccaaccagc cagcgacgta
    35761 aatcaccgtc ggtaaagacg cctttcacca gtcgttgatc atcgcacact gccaccagac
    35821 ctaaaccggt gcggctaagt tccagcatcg cgtccatcac gctggcggtt aacgccacct
    35881 gcgggatggc atcgtcacga cgcataagat gatgcacttt gttcagcaag cgagcgccca
    35941 gcgcgccggc tggatgtgaa cgggcaaaat cttcttcatt aaaaccacgt gcctgcatga
    36001 ctgccatcgc cagcgcgtcg cccatcatca gggtattcac ggtgctggag gtcggcgcaa
    36061 ggtgcatcgg gcaggcttcg cgttctacgg agatatccag caccgctttc gccgccaggc
    36121 ccagcggtga cgtcggtttt ccggtcattg ccagcagagc gatagatttg tcttccagac
    36181 gcggaataat gagatccagt tccttcgcgc caccggaata agagataaac agcatcacat
    36241 cgcggctttc aatcatcccc agatcgccgt gcagtgcttc tgccggatgg acaaaaaaag
    36301 ccggtgtgcc ggtgctggca agcgttgcgg cgattttctt accaatgtgg cccgatttgc
    36361 caattcccga aaccaccact ttgccttcgc agtgcaggat gatattggcg gcgcgaacga
    36421 aatcatcgcc cagacgttcc ggtaaatggc ttgcttcctg caactccagc attaacgtct
    36481 gacgtcccgc gttcagtagt gcttcactca ttgctctctc cggttaaggt ccatttattt
    36541 attccagcca gggattggta gccagattga acgctcatta tccaatgcca tggctggtga
    36601 aacaactgat gtaaatttag ttacaacatt ctttaaaaaa aaatatgaac tgaaagaaga
    36661 tactagagag aaacagagca ctgaaaacag acaaccgctt ccttgtgcgg caggggatgc
    36721 tttaccatac ttgcccctgg ttgaatctgt taaatggacc cctcatgttc aagcgtcgtt
    36781 atgtaacatt gcttcccctt tttgtgttgc ttgccgcctg tagcagcaag ccaaaaccta
    36841 ctgagactga aacgaccacc ggaacgccgt ctggcggctt cctgcttgag ccgcagcaca
    36901 atgtgatgca gatgggcggt gacttcgcca ataacccgaa tgcccagcag ttcatcgaca
    36961 aaatggtgaa caaacacggt ttcgatcgtc agcagttgca ggaaattctc tcccaggcga
    37021 aacgtctgga ttcggtactg cggctgatgg ataaccaggc accaaccaca tcggtgaaac
    37081 cgccatcagg tccgaacggc gcatggctgc gttatcgcaa aaaatttatt acgccggaca
    37141 acgtgcagaa cggtgtggtt ttctggaatc agtatgaaga tgcgttgaat cgcgcgtggc
    37201 aggtgtatgg tgtaccgccg gaaattatcg tcgggattat cggcgttgaa acccgctggg
    37261 ggcgcgtgat ggggaaaact cgcatcctcg atgcgctggc aacgctgtca tttaactacc
    37321 cacgccgcgc ggagtatttt tctggcgagc tggaaacctt cctgctgatg gcgcgcgacg
    37381 agcaggacga tccgctcaat ctgaaaggtt cctttgccgg ggcgatgggc tacggtcagt
    37441 ttatgccttc ttcttacaaa caatatgcgg tagatttcag cggcgacggg catatcaact
    37501 tgtgggatcc ggttgatgcc atcggtagcg tggcgaatta tttcaaagcg cacggctggg
    37561 tgaaaggcga tcaggttgcg gtgatggcaa acggtcaggc tccaggcttg ccaaatggct
    37621 tcaaaaccag gtacagcatt tcgcagcttg ccgcggcagg tttaacgcca cagcagccgc
    37681 tgggcaacca tcaacaagcc agcctgctgc gtctggatgt tggcaccggc taccagtact
    37741 ggtacggtct gccgaacttc tacaccatca cccgttataa ccacagcacc cattacgcaa
    37801 tggcggtctg gcagttagga caagccgtgg cgctggcgcg cgtacagtaa ctatttgcaa
    37861 gaggggggcg tgagcttccc tctggctaat cagctttttt ctgaatcctc ctcgtaaaat
    37921 tgcaacgcca acaccatctt cctgacgaaa gtgttatctt gtccggcata aatttttgac
    37981 tgacagaggt tgtgatgact gacagtgaac tgatgcagtt aagcgaacag gttgggcagg
    38041 cgctgaaagc ccgtggcgca accgtaacaa ccgctgagtc ttgtaccggc ggttgggtag
    38101 cgaaagtgat taccgatatt gccggtagct ccgcctggtt tgaacgcgga tttgtcacct
    38161 acagtaacga agccaaagcg cagatgatcg gcgtgcgcga agagacgctg gcgcagcatg
    38221 gcgcggtgag tgaacccgtc gtggtggaaa tggcgatagg cgcactgaaa gcggctcgtg
    38281 ctgattatgc cgtgtctatt agtggtatcg ccgggccgga tggcggcagt gaagagaagc
    38341 ctgtcggcac cgtctggttt gcttttgcca ctgcccgtgg tgaaggcatt acccggcggg
    38401 aatgcttcag cggtgaccgt gatgcggtgc gtcgtcaggc tactgcgtat gcattgcaga
    38461 ccttgtggca acaatttcta caaaacactt gatactgtat gagcatacag tataattgct
    38521 tcaacagaac atattgacta tccggtatta cccggcatga caggagtaaa aatggctatc
    38581 gacgaaaaca aacagaaagc gttggcggca gcactgggcc agattgagaa acaatttggt
    38641 aaaggctcca tcatgcgcct gggtgaagac cgttccatgg atgtggaaac catctctacc
    38701 ggttcgcttt cactggatat cgcgcttggg gcaggtggtc tgccgatggg ccgtatcgtc
    38761 gaaatctacg gaccagaatc ttccggtaaa accacgctga cgctgcaggt gatcgccgca
    38821 gcgcagcgcg aaggtaaaac ctgtgcgttt atcgatgctg aacacgcgct ggacccaatc
    38881 tacgcacgta aactgggcgt tgatatcgac aacctgctgt gctcccagcc ggataccggc
    38941 gagcaggcac tggaaatctg tgatgccctg gcacgttctg gcgcagtaga cgttatcgtc
    39001 gttgactccg tggcggcact gacgccgaaa gcggaaatcg aaggcgaaat cggcgactct
    39061 cacatgggcc ttgcggcacg tatgatgagc caggcgatgc gtaagctggc gggtaacctg
    39121 aagcagtcca acacgctgct gatcttcatc aaccagatcc gtatgaaaat tggtgtgatg
    39181 ttcggtaacc cggaaaccac taccggtggt aacgcgctga aattctacgc ctctgttcgt
    39241 ctcgacatcc gtcgtatcgg cgcggtgaaa gagggcgaaa acgtggtggg tagcgaaacc
    39301 cgcgtgaaag tggtgaagaa caaaatcgct gcgccgttta aacaggctga attccagatc
    39361 ctctacggcg aaggtatcaa cttctacggc gagctggttg acctgggcgt gaaagagaag
    39421 cttatcgaga aagcaggcgc gtggtacagc tacaaaggtg agaagatcgg tcagggtaaa
    39481 gcgaatgcga ctgcctggct gaaagataac ccggaaaccg cgaaagagat cgagaagaaa
    39541 gtacgtgagt tgctgctgag caacccgaac tcaacgccgg atttctctgt agatgacagc
    39601 gaaggcgtag cagaaactaa cgaagatttt taatcgtctt gtttgataca caagggtcgc
    39661 atctgcggcc cttttgcttt tttaagttgt aaggatatgc catgacagaa tcaacatccc
    39721 gtcgcccggc atatgctcgc ctgttggatc gtgcggtacg cattctggcg gtgcgcgatc
    39781 acagtgagca agaactgcga cgtaaactcg cggcaccgat tatgggcaaa aatggcccgg
    39841 aagagattga tgccacggca gaagattacg agcgcgttat tgcctggtgc catgaacatg
    39901 gctatcttga tgacagccga tttgttgcgc gctttatcgc cagccgtagc cgcaaaggtt
    39961 atggacctgc gcgtattcgt caggaactga atcagaaagg tatttcccgc gaagcgacag
    40021 aaaaagcgat gcgtgaatgt gacatcgact ggtgcgcact ggcgcgcgat caggcgacgc
    40081 gaaaatatgg cgaacctttg ccaactgtct tttcagaaaa agttaagatc cagcgttttc
    40141 tgctctatcg tggctatctg atggaagata tccaggatat ttggcgaaat tttgccgact
    40201 gaacgcatac gggattttac ttcccagtca agaaaactta tcttattccc acttttcagt
    40261 taccagccgg gcggttaacg acacgcaacg gctggtagcg atatttcgtt agcttgattt
    40321 caggataatt atgagcaaga gcaccgctga gatccgtcag gcgtttctcg actttttcca
    40381 tagtaaggga catcaggtag ttgccagcag ctccctggta ccccataacg acccaactct
    40441 gttgtttacc aacgccggga tgaaccagtt caaggatgtg ttccttgggc tcgacaagcg
    40501 taattattcc cgcgctacca cttcccaacg ctgcgtgcgt gcgggtggta aacacaacga
    40561 cctggaaaac gtcggttaca ccgcgcgtca ccataccttc ttcgaaatgc tgggcaactt
    40621 cagcttcggc gactatttca aacacgatgc cattcagttt gcatgggaac tgctgaccag
    40681 cgaaaaatgg tttgccctgc cgaaagagcg tctgtgggtt accgtctatg aaagcgacga
    40741 cgaagcctac gaaatctggg aaaaagaagt agggatcccg cgcgaacgta ttattcgcat
    40801 cggcgataac aaaggtgcgc catacgcatc tgacaacttc tggcagatgg gtgacactgg
    40861 tccgtgcggc ccgtgcaccg aaatcttcta cgatcacggc gaccacattt ggggcggccc
    40921 tccgggaagt ccggaagaag acggcgaccg ctacattgag atctggaaca tcgtcttcat
    40981 gcagttcaac cgccaggccg atggcacgat ggaaccgctg ccgaaaccgt ctgtagatac
    41041 cggtatgggt ctggagcgta ttgctgcggt gctgcaacac gttaactcta actatgacat
    41101 cgacctgttc cgcacgttga tccaggcggt agcgaaagtc actggcgcga ccgatctgag
    41161 caataaatcg ctgcgcgtaa tcgctgacca cattcgttct tgtgcattcc tgatcgcgga
    41221 tggcgtaatg ccgtccaatg aaaaccgtgg ttatgtactg cgtcgtatca ttcgtcgcgc
    41281 agtgcgtcac ggtaatatgc tcggcgcgaa agaaaccttc ttctacaaac tggttggtcc
    41341 gctgatcgac gttatgggct ctgcgggtga agacctgaaa cgccagcagg cgcaggttga
    41401 gcaggtgctg aagactgaag aagagcagtt tgcccgtact ctggagcgcg gtctggcgct
    41461 gctggatgaa gagctggcaa aactttctgg tgatacgctg gatggtgaaa ctgctttccg
    41521 cctgtacgac acctacggct tcccggttga cctgacggct gatgtttgtc gtgagcgcaa
    41581 catcaaagtt gacgaagctg gttttgacgc agcaatggaa gagcagcgtc gtcgtgcgcg
    41641 cgaagccagc ggctttggtg ccgattacaa cgcaatgatc cgtgttgaca gtgcatctga
    41701 atttaaaggc tatgaccatc tggaactgaa cggcaaagtg accgcgctgt ttgttgatgg
    41761 taaagcggtt gatgccatca atgcaggcca ggaagctgtg gtcgtgctgg atcaaacgcc
    41821 attctatgcg gaatccggcg gtcaggttgg tgataaaggc gaactgaaag gcgctaactt
    41881 ctccttcgcg gtggaagata cgcagaaata cggccaggcg attggccaca tcggtaaact
    41941 tgctgcgggt tctctgaaag tgggcgacgc tgtgcaggct gatgttgatg aggctcgtcg
    42001 cgcccgtatt cgtctgaatc actccgcaac gcacctgatg cacgctgcgc tgcgccaggt
    42061 tctgggtact catgtatcgc agaaaggttc actggttaac gacaaagtgc tgcgcttcga
    42121 cttctcacac aacgaagcga tgaaaccaga agagattcgt gcggtcgaag acctggtgaa
    42181 cgcacagatt cgccgtaact tgccgatcga aaccaacatc atggatctcg aagcggcgaa
    42241 agcgaaaggt gcgatggcgc tgtttggcga gaagtatgat gagcgcgtac gcgtgctgag
    42301 catgggcgat ttctccaccg agctgtgtgg cggtactcac gccagccgca ctggtgatat
    42361 tggtctgttc cgcatcatct ctgaatcggg tactgctgca ggcgttcgtc gtatcgaagc
    42421 ggtaaccgga gaaggcgcta tcgccaccgt tcatgcagac agtgatcgct taagcgaagt
    42481 cgcgcatctg ctgaaaggcg atagcaataa tctggctgat aaagtgcgtt cagtactgga
    42541 acgtacgcgt cagctggaaa aagagttaca acagcttaaa gaacaagctg ccgcacagga
    42601 gagcgcaaat ctttccagta aggcaattga tgttaatggt gttaagctgt tggttagcga
    42661 gcttagcggt gttgagccga aaatgttgcg taccatggtt gacgatttaa aaaatcagct
    42721 ggggtcgaca attatcgtgc tggcaacggt agccgaaggt aaggtttctc tgattgcagg
    42781 cgtatctaag gacgtcacag atcgtgtgaa agcaggggaa ctgattggta tggtcgctca
    42841 gcaggtgggc ggcaagggtg gtggacgtcc tgacatggcg caagccggtg gtacggatgc
    42901 tgcggcctta cctgcagcgt tagccagtgt gaaaggctgg gtcagcgcga aattgcaata
    42961 atataagcgt caggcaatgc cgtggactcg cttcacggca ttcgcattaa cgctatcgac
    43021 aacgataaag tcaggttgaa gttgtgtata tcggctaaac ttaggtttaa cagaatgtaa
    43081 tgccatgact gcttagatgt aatgtgtttg tcattgctta ctttttggcg ttatatgatg
    43141 gataatgccg ggatacagag agacccgact cttttaatct ttcaaggagc aaagaatgct
    43201 gattctgact cgtcgagttg gtgagaccct catgattggg gatgaggtca ccgtgacagt
    43261 tttaggggta aagggcaacc aggtacgtat tggcgtaaat gccccgaagg aagtttctgt
    43321 tcaccgtgaa gagatctacc agcgtatcca ggctgaaaaa tcccagcagt ccagttacta
    43381 atctttccgc gtctcatctt tatcggtgag acgcaccctc aaaatttctc tttcactcca
    43441 ttgtcttttc gctttactcc cgttcattca acttaagtct ccattttttt gcattactgc
    43501 tatctgtcag acctccgttt ttctgttgat aaaacactct ttttgacgtt tttacagact
    43561 aattgagcgt gaagtgtgca aacgataaaa gtgtgggaaa aattgtttga cttataagtc
    43621 tcagaaagta atatgtgcgc cacgcagcga cgatgagcga taaacaagtt cttcgaagca
    43681 ctcgtaagag gcgtgtggtg aggtggccga gaggctgaag gcgctcccct gctaagggag
    43741 tatgcggtca aaagctgcat ccggggttcg aatccccgcc tcaccgccat ttgcatccgt
    43801 agctcagctg gatagagtac tcggctacga accgagcggt cggaggttcg aatcctcccg
    43861 gatgcaccat ctcttacttg atacggcttt agtggcggta tcaaaaaatc tgcagtaaag
    43921 taagtttccc gatgcatccg tagctcagct ggatagagta ctcggctacg aaccgagcgg
    43981 tcggaggttc gaatcctccc ggatgcacca tctcttactt gatatggctt tagtagcgat
    44041 atcaatatca gcagtaaaat aaatttttcc gatgcatccg tagctcagct ggatagagta
    44101 ctcggctacg aaccgagcgg tcggaggttc gaatcctccc ggatgcacca tattctccgt
    44161 aaccttcagc aatgaaggta tcgaagaggt agcggtatta accgcacgcc agcagcggcc
    44221 cgaagggcga acgccagtga gtcatcctcc cggatgcacc atattctcca aaaaatttcc
    44281 ttcaaaaata tctcatttct aaataactac tttatccatt tcctgaattt tcttctcctt
    44341 ctccatcagc gacaaaatca agcaattacg ctaaagtagc gtcatatttt tcgacttgcg
    44401 agaagaccat gtacgagcgt tatgcaggtt taatttttga tatggatggc accatcctgg
    44461 ataccgagcc tacgcaccgt aaagcgtggc gcgaagtatt agggtactac ggtcttcagt
    44521 acgatgttca ggcgatgatt gcgcttaatg gctcgcccac ctggcgtatt gctcaggcga
    44581 ttattgagct gaatcaggcc gatctcgacc cgcatgcgtt agcgcgtgaa aaaacagaag
    44641 cagtaagaag tatgctgctg gatagtgtcg agccgcttcc tcttgttgaa gtggtgaaaa
    44701 gctggcatgg tcgtcgccca atggctgtag gaacggggag tgaaagcgcc atcgctgagg
    44761 cattgctggc acacctggga ttacgccgtt attttgacgc cgtcgtcgct gccgatcacg
    44821 tcaaacacca taaacccgcg ccagacacat ttttgttgtg cgcgcagcgt atgggcgtgc
    44881 aaccgacgca gtgtgtggtc tttgaagatg ccgatttcgg tattcaggcg gcccgtgcag
    44941 caggcatgga cgccgtggat gttcgcttgc tgtgagtgaa gcgttatcgc ttttctcgtt
    45001 gtttgccagt agttttctca gcgctacact attacccggc aactccgaag tcgtgctggt
    45061 ggcaatgttg ctttccggag tgagtcatcc ctgggtttta gttttaacag caacaatggg
    45121 taatagcctt ggagggttaa ctaacgttat ccttgggcgt ttctttccat tgcgtaaaac
    45181 atcgcgctgg caagagaaag ctaccggctg gctgaaacgc tatggtgcag tcacgctatt
    45241 attaagctgg atgcccgtgg ttggcgattt actgtgtctg ttagcgggat ggatgcgcat
    45301 ctcgtgggga ccggtaatct tttttttgtg ccttggtaaa gcgttacgct atgttgcagt
    45361 tgcagcagct accgttcagg gcatgatgtg gtggcactaa ttgtaggcct gcacatatgg
    45421 tcaccattac agttatgcta attaaaacga ttttgacagg cgggaggtca atttgatccc
    45481 ggacgtatca caggcgctgg cctggctgga aaaacatcct caggcgttaa aggggataca
    45541 gcgtgggctg gagcgcgaaa ctttgcgtgt taatgctgat ggcacactgg caacaacagg
    45601 tcatcctgaa gcattaggtt ctgcactgac ccataaatgg attactaccg attttgcgga
    45661 agcattgctg gaatttatta caccagtgga tggtgatatt gaacatatgc tgacctttat
    45721 gcgcgatctg catcgctata cggcgcgcaa tatgggcgat gagcggatgt ggccgttaag
    45781 tatgccatgc tatatcgcag aaggtcagga catcgaactg gcacagtacg gcacttctaa
    45841 caccggtcgc tttaaaacgc tgtaccgtga agggctgaaa aatcgctacg gcgcgctgat
    45901 gcaaaccatt tccggcgtgc actataattt ctctttgcca atggcattct ggcaagcgaa
    45961 gtgcggtgat atctcgggcg ctgatgccaa agagaaaata tctgcgggtt atttccgtgt
    46021 tatccgtaac tactatcgct tcggttgggt cattccttat ctgtttggtg cgtctccagc
    46081 gatttgttct tctttcttgc aaggaaaacc aacgtcgctg ccgtttgaga aaaccgagtg
    46141 cggtatgtat tacctgccgt atgcgacctc tcttcgtttg agcgatctcg gctataccaa
    46201 taaatcgcaa agcaatcttg gtatcacctt caatgatctc tacgagtacg tggcgggcct
    46261 taaacaggca atcaaaacgc catcggaaga gtacgcgaag attggtattg agaaagacgg
    46321 taagcgactg caaatcaaca gcaacgtgct gcagattgaa aacgaactgt acgcgccgat
    46381 tcgtccaaaa cgcgttaccc gcagcggcga gtcgccttct gatgcgctgt tacgtggcgg
    46441 cattgaatat attgaagtcc gctcgctgga catcaacccg ttctcgccga ttggtgtaga
    46501 tgaacaacag gtgcgattcc tcgacctgtt tatggtctgg tgtgcgctgg ctgatgcacc
    46561 ggaaatgagc agtagcgaac ttgcctgtac acgcgttaac tggaaccggg tgatcctcga
    46621 aggtcgcaaa ccgggcctga cgctgggtat cggctgcgaa actgtgcagt tcccgttacc
    46681 gcaggtgggt aaagatctgt tccgcgatct gaaacgcgtc gcgcaaacgc tggatagcat
    46741 taacggcggc gaagcgtatc agaaagtgtg tgatgaactg gtcgcctgct tcgataatcc
    46801 cgatctgact ttctctgccc gtatcttaag gtctatgatt gatactggta ttggcggaac
    46861 aggcaaagcg tttgctgaag cgtaccgtaa tctgctgcgt gaagagccgc tggaaattct
    46921 gcgcgaagag gattttgtag ccgagcgcga ggcgtctgaa cgccgtcagc aggaaatgga
    46981 agccgctgat accgaaccgt ttgcggtgtg gctggaaaag cacgcctgac agaaaagaaa
    47041 aaggccactc gtgagtggcc aaaatttcat ctctgaattc agggatgatg ataacaaatg
    47101 cgcgtctttc atatactcag actcgcctgg gaacaaagag ttcagaaaat tttaaaaaaa
    47161 attaccggag gtggctaaat gccgttgtta gatagcttca cagtcgatca tacccggatg
    47221 gaagcgcctg cagttcgggt ggcaaaaaca atgaacaccc cgcatggcga cgcaatcacc
    47281 gtgttcgatc tgcgcttctg cgtgccgaac aaagaagtga tgccagaaag agggatccat
    47341 accctggagc acctgtttgc tggttttatg cgtaaccatc ttaacggtaa tggcgtagag
    47401 attatcgata tctcgccaat gggctgccgc accggttttt atatgagtct gattggtacg
    47461 ccagatgagc agcgtgttgc tgatgcctgg aaagcggcaa tggaagacgt gctgaaagtg
    47521 caggatcaga atcagatccc ggaactgaac gtctaccagt gtggcactta ccagatgcac
    47581 tcgttgcagg aagcgcagga tattgcgcgt agcattctgg aacgtgacgt gcgcatcaac
    47641 agcaacgaag aactggcgct gccgaaagag aagttgcagg aactgcacat ttagtcagtt
    47701 taagcagaag aggccgggct ggtgaaaaca ccgcccggct tttgttacct gtcagtcatc
    47761 cgatattcca cgctatgtgc ttcatcaggt tgaggaactt ccagccttga ggccatccgc
    47821 atcacgaccg cttcaggaac tgcatactcg cgtctggcat tttgctgctt ccattgcgcc
    47881 catggcactt caagataaac gatttttact cgtgcgccgt aagccgtaaa cagactgatg
    47941 agctggctgc gcagctggcg ggtaatgttc gttgcattcc agatgaacgg ctttttctgg
    48001 cgtaaaaaaa cgcgcgcctc ttcttttgcc tgttgcacaa tacgcccggt agcggttttg
    48061 tcgtccggac tggcattgat tcgccgccgc atatcatcaa ggctgataac gtccattccc
    48121 tggcactgtt cattgatata gcgatctttg cccatacccg gtaagccaca cagcaaaatg
    48181 acctcaaaag gttcagcctc ccagggaaca aaatcaggag aactttgctc atgggtcagg
    48241 taatgccagc gtgcggaatc agaaacgaac gggcgcattt ttccccagca ttgttgctca
    48301 tggcaaaaca gctcgaacag atcgatgcgt tccagcattg attgctgatc cgggctttgg
    48361 cgaccaagca gatcggcacg agcaagcaaa gcgagcagac gcgtgtcgat gcgcattgca
    48421 gccgtgagca acaaacgctc cggttccggg cgttccagta accatagcgg taacccatgc
    48481 agacgcacca gtgcaacaat ttgctcgcgc agcacgaacg gcgttgggat atcgcgccat
    48541 aaaatttgtc gggccgtcag ttcgccccga cgagcatgac caggagactg aatacgtccg
    48601 ttttcctgca cggtagtgct gcgcttttca acatcatgca ataacgccgc cgcccataaa
    48661 acttcctgct gttgagtggg gagttgctgg aattctggca gagtaataag ggcgttaagc
    48721 accatttcgg tatgtacgcc gacgtcgcct tcaccatgat gctcagggtc ttgtggcgtg
    48781 tgatgcatct cctcaaccca gctaaaacgc tgacgtagcg ccgaccagcg tttatcgtcg
    48841 gtaagttgcc aaatcatgac tgttctccat aaacgaactc atgggccatc cgcgcccgtt
    48901 gccagtggcg tttccagtgc acagtggttt tgacatgatt tttgcgcaca tacttgaaga
    48961 cgttatgcga aaagtcagcg acagaaaacg catcatgatt tcggctgaca atgccttcca
    49021 gcgtacaggg ttgccaggtt tgcgtatccc agggatcgaa tgtgccgcgc gcattggcca
    49081 gcgccagaaa atcacgttgc cagctttttt catcattccc cggctgcggg ccggagattt
    49141 cgggaacgca ggggaaatca aacagagcgg cgtaaaactg cacctcttcc cagctcagcc
    49201 acatatcctg gcagcgaacg gcaaacaggt aaaagtcctg ttccagcgcg cggtattcaa
    49261 tggaatgaac ggcgtagaga ttttcaccaa acaactccag gtcgccgaga tcgtttttca
    49321 gcagttgcca gcgttggcga attttatagg tccatgccga ctgcgtaggc gtggcatggg
    49381 agcgggcaaa cacgccgtag cggttaaggc agttattttc gccatcgagt ttttctgtgt
    49441 gcaccaattg cgtgatggtc tgtaaatcct gccagtaatc ggtgttaatg cggtcatcgc
    49501 tggtggtgcc tggagagaag gggtaatgcc aggttctccc gtattttcgt tgggtattca
    49561 taataatcca ttattttgtg tgtaataaat gtacgcccgc gagggtgcga gttttggggt
    49621 taaaagaaaa atgtgagtat cacccgcgaa agcgagggcg aaggaaaaag agaattaaca
    49681 acctgagcga atgctcagaa ccggctaatg gtgaatggaa gagtttgggg cggcgaaaag
    49741 atacaccgaa tgagtgtata aaacaaccgt ttgcagaatg agcaaataaa aagggagccg
    49801 cagctccctt tggcaacctt agtgcgcacc acctccgccg ccacctgcgc caaatggcgg
    49861 tttagcaaac cacaccagcc ccagcaggac gaggaatatc ccggctgaca tccagaagat
    49921 ctcattggcg gaaataatca gcccctgatt ggttatctgc tgggcaatcc agcctgatgc
    49981 ctgctgttgc gtcataccaa gcccttccag ttgactgtac atcgcctgag cattcgggtt
    50041 gaacgggttt accgactcag tcaactgtgc atggtgcagt gactcgcggt tggtccacat
    50101 ggtcgtggtt atcgacgtgc cgatagaccc cgccagcgtt cgcgtaaagt tggagaggct
    50161 cgatgccgcc gccagtcgtt ccggtggcaa accggacagc gtaatggtgg tcagcggcat
    50221 aaagaagcag gccaccgcaa aaccctggat aaactgtggc caggccgacg cgccaaaatc
    50281 catacccggt tcaaaggtat aggcacgcca gtagaagcag acggcataca taataaagct
    50341 gaaggttacc agtcggcgca tatccagttt atgcgcaaag cggccgataa tcggcgacag
    50401 gatcaccgga ataatcccta ccggcgcaga ggccagccct gcccacgtcg ccgtgtaacc
    50461 gtagacctcc tgcaacaact gcggcagcag aacaatcgcg ccgaagtaga gcatatacgc
    50521 gaggctgata cacaagcagc cgatggtgaa gttgcgcgac ttaaacaacg acagatcgac
    50581 tatcgggtta tcgtcggtca gctcccagac aatcaggaag cagatagcca ccaccgccac
    50641 cacggtgagg atgataattt cctgtgatga gaaccagtcc agctctttac cgcggtcgag
    50701 cataatctgc aggctgccga taccaataac cagcagtgcc agccccacgg catcaatccg
    50761 ccgccgttcg gtgcgggttt cacgtccgcg cagagtttgc agtgtcatca acaccaccgc
    50821 cacgccaatc ggcacgttga tgaagaatat ccagccccag tggtaattat cactgatata
    50881 accgccgagg atcgggccgc aaattggcgc aacaatcacc gtcatcgacc acaacgccag
    50941 cgcgatcgag cgtttggctg gcgggtagtt attcagcaat agactttgcg aaagcgggat
    51001 cagcggcccg gcgacaatcc cctgaatcac gcggaagaag atcagcatat tcaggctgct
    51061 ggagacacca cacgcccacg acgcaatagc aaaggcgatg gttgaccaga ggaacaattt
    51121 cacttccccg acgcgctttg ccagccagcc ggtaagcggg atcgagatgg cattcgccac
    51181 cccgaaagaa gtgatcaccc acgttccctg actgagtgat gagcccagat tcccggcgat
    51241 agtggggatc gccacgttag caatggtgga gtccagcacc tgcatgaatg tcgccagtga
    51301 cagcgcaatc gtcataatga ccagttgtgc gccttccagc ggtttttgct gttgcatcac
    51361 acgcacctct ggattagcca gcgttagctt ttacgatatc gtcgatcagt ttattgacag
    51421 gtgccaggct gatttcacgc gcggtgctta ccgcgaccgg tgtggaacgt actttatttg
    51481 ccagtacctg accgtcacgg ttagtggtat tgacactcac cagcgtggat aaaccgatac
    51541 gcagcgggta ttgctccagc tgtttctggt ccagttcaat acgcacaggc agacgctgaa
    51601 cgactttgat ccagttaccg gtggcgtttt gcgccggaag cagtgagaac gcgctacctg
    51661 tgcccatatc cagaccaacc actttaccgg tgtatttcac atcatcgccg taaatatcgg
    51721 tggtgatggt gaccggctga ccgatacgca tattggcaat ctgcgtctct ttaaagttgg
    51781 catccaccca catattggtg gctggaacga ccgccatcag cggcgtcgtt gggctaattt
    51841 gcgccccagg ctgtaccgcg cggcgggaga cataaccggt catcggactg acaatacggg
    51901 tacgctccag cgccagccag gcgttacgta cttcggtggc agcctgttgc acggcaggct
    51961 gatcttccag tttcgtcccc agaatcatcg cctgattggc attgtattgt tgaatagcga
    52021 cgtccagttg cgcctgggca ctggtgacgg cgtcgcgggc gtgttgcagc tcttcgcgac
    52081 caatcaggtt ggcattgccc agcggcacac ggcggttgta gtcgctttgt gctttcgcga
    52141 gggcgatttt ttgcacctca atattcgcct gcaactgttt gctgttaatc atcagctggt
    52201 gggtttggcg aacgctggaa gtcagtgcag ttttggcttt ttcaaatgcc tggcgagcat
    52261 ctgtcgggtc gagagtgacc agcacgtcgc cttcttttac aaaatcggtg ttatcggccc
    52321 agactttcgt cacgctgcca gacacctgag acataatttg aatctgattc cctgccacgt
    52381 atgcgtcatc ggtttcttcg aaatgacgca gtaccaaaaa ccaataaatc cctatcgcta
    52441 cggcaataat tataaagagc aaggtgagaa ggaggagcag acgcttacgt ttgccgctct
    52501 tctttaccgg ttgctgcggg gtttgagtct ccgcatttgc gctcatattg ttctccacga
    52561 tcttcttaac tcatcggctg agccgacctg cttgccaaaa aggccagcat gttacgatgc
    52621 tggccggtta ttttttcttt tataaatctg gatttttgag cgagatgacg cgttagctca
    52681 tcgcttcgag aaccacaccg tcctgttcca tctggtcgag acgggagagc aatttgcggg
    52741 tgatttgctc gagctggtct ttttctgttg tgctgagcgc ggaccagagt tgatgcaggc
    52801 agttatgctg cggcggtaaa acctcgcgca aaaactcgtg acctttttcc gttaattgca
    52861 gatgcaggca gcggcgatcg ttatcgcttt cacgacgttc gatccaaccg cgtttttcca
    52921 gttcatcggc aatacgcgtc gcgttggtac gggatgatcc aagagcacaa ctcaattcag
    52981 aaggctgaat actgtggttt tcctgagact ccagcgtaat caacgccata aacaacgtct
    53041 cgttaatccc ctgagccttc agcattttat tgcggttttc cagcagcttg ctttgcatat
    53101 gcatgcaaag acgggtcaga aggatctcct gataaggaaa atcttcgtgg cggctggcgc
    53161 gaaattttag catttgttca atgggcgtaa acgaactatc catttgggta tgacctcatt
    53221 aatttcagcc gatatagtaa cgacagtgac aaataaagta aatgtattgt tttagaaaaa
    53281 tgattcttgt gggttatata atcgccatca ctttccaggc tagcccatag gccagcgcac
    53341 taagcagtgt tgggatgata atgctgcgtg ttttatagaa actggcaccc agtaccgcga
    53401 agccgaccag tgtgggcacg aaacggcttg tatcgtgcat cacttctggt gcggtagaga
    53461 caaccagcag agcgcatatc gaggcgatgc caatggtgtc gagcaaaata cctaccgcgc
    53521 cacgtttggt tgggcgggca ttacccacac gcaggcgcag cggcagatag cggaagcaat
    53581 aattcgccgc gccaaccagt aacccaagca gcagaacctc atagctcatc gggcgctcct
    53641 tgccagaatg cctggattaa cgcagtgagg cagccacaga caatgcctgc cagaatggcg
    53701 acgggaatag aaaatagcgt tacgcctgca agggcaccaa ctaacgctgc ggtaacgcaa
    53761 agagattgtt tgcgctggaa agaggcgagc aggaaactca taaagagtgc cggaagcata
    53821 aaacctaatg ctgcttcaac ggcgggataa ccttgcagca agccgctgcc ggagaatgcc
    53881 cctattaccg taccaaatac ccacgatgac catgaactga aggcaatgcc gatcatccag
    53941 ttttcgctcc agcggcgatt attgcgtacc agttttgcgg ttgcggcggc aaacacctca
    54001 tccgtcaggc caaacgccca cagggcggtt ttcgattttt gcagacgctg aataatacgg
    54061 ctacgcagtg acgggccata caaaacatgg cgaacatcca ttgccatgac ggtcagtgca
    54121 gcaacccaca aactactccc ggctgccagc atcgcggtaa tgacgaactg gctcgcgcct
    54181 gcataaatga tgcaggagaa aaaaacgctt tcgagaggag agaatcccag acgggtcgca
    54241 ttcagaccga acgcaaaggc caccggaata taactaataa caatcggtaa actgtctttg
    54301 catccttcca tgaaggtcgc cgaaccagga gcaggctgtg gagtagggct ttccatagag
    54361 tgtacgctta acgattgtta atattataaa tagactgaat gaatatctta accttatcag
    54421 actgatgggc ttcttaacac ccttataagt gtaaagccac gaaaacggtt gctgattgct
    54481 taatttgcgg cttcctgacg atgaaaacct cgccaccaga ccagtaagtt aaccagggca
    54541 atcgtcgcgc cagccagaca cacgccagcc caactgccat gttgccaggc tgaggctgaa
    54601 attagcgaac cagcggcacc gccaataaag tagctggtca tgtaacctgc ggtcaggcga
    54661 ttacgcgcat cagggtgtat tcgataaatt accgtctggt tagtgatatg cacgccctgc
    54721 acggtgaggt ccagcaccag gatgccgata atcagcgcca gtacggaagt gtgaccaaac
    54781 cagattgcca gccatgaaag taacagcagc agcagaccga aagttgtggt gtggtgcgat
    54841 ttgcccttat cggcaaaacc gcccgccgaa cgagcgccca acgctccggc agctcccgca
    54901 agcccaaaca gaccaattac accatcgctg tagttaaaag gtggagcggc aagcaaaaag
    54961 gccattgagg tccagagaat gctgaagttg gcaaaggtca ggcagcccag caacgcgcgg
    55021 gtgcgcagga tcttattgct gataaacatg ctgaaaacgg aacccaacaa ctgtgggtag
    55081 ttgaggtggg tttctgattt catttgtggc agaccacgcc ataatgccag cgccatcagt
    55141 gccattaaca tcgaagcaac ccaaaagacg gtgcgccagc cgccgagatt cgccagcaat
    55201 ccggcaacgg tccgtgcgag taagatcccc aatagcagac cgctcataat ggtgccaacc
    55261 actttgccgc gtttgtccgg tgaagccagc gtcgctgcca gtggaaccag aatttgtgcc
    55321 acgactgaaa ataaaccggt taatgctgta ccgaggatca tcatcgccag cgactgactg
    55381 ctggcagtga ttaacatgcc gcctgctgcc agtaaggtca tcgagacaat caggcggcgg
    55441 cgttcaaaca tatcaccgag gggaacaaga aacagcagac ctgcggcata gcccaactgc
    55501 gcggcggtaa caataaagcc tgccgaactg gcggaaaggg aaaagttacg cgcgatggtg
    55561 tcgagcaatg gctgggcgta atagttactg gctaccgcca gaccggtggc gatagacatc
    55621 agcacgatca gcgccgggct aagctcatga ttaggtttag tcattatttt tgttcatcag
    55681 gtgatgatta agctggaaat caataatagc ggatgttgat gaatatggga tgaaagatag
    55741 attgttgaaa tgtgcggtgt ggtttgtagg tatgataaga cgcgccagcg tcgcatcagg
    55801 cattgtgtac agagcggcgg atgaggcaaa aacgccttat ccgcccgaat aaaaattact
    55861 tctgcgctgc cagcgcttca ttcacccagc catcgaactg ctgctggtgg gctttgatcc
    55921 aaccatcaac gtggccctga atatcgtctt ctgaggcttt gccgtcatgc atgatggcgt
    55981 tctgggcgtt aatatcagcc actggtaact gcataatggc aaacagtttc gctgctgccg
    56041 ggtttttctc ggcccaggct ttgttggcaa cgatatgcat ggtactgacc gggaagccgt
    56101 aattcgcgcc attcggcagt ttggtatcgg catttttatc gcccggcagt gcggagaacg
    56161 gcacctgcaa ccagaccaca tctttccccg gtttcagttc gttactcacc cagtacggcg
    56221 tccaggtgta gtaaaacacc ggcttaccct ctttgtaacg actgatggtg tcagccatca
    56281 tcgctgcgta gttcccctga ttatgcgtca cggtattggt cagtccatac gcggcaagct
    56341 ggtggttgat cgctccttcg cagccccagc ctgggttaca gccggttaaa tccgctttac
    56401 cgtcgccgtt ggtatcgaac agtttggcga tcttcggatc tttaagttgc gcgatgttgg
    56461 taattttata ctggtcggca gttttcttat cgatcaggta accctgtgcc gcgccgttaa
    56521 caaatactcc ttcacgataa aatttcttat cgccaccggc agcttcgtac atgttgtcat
    56581 gcagtggcgt ccagttcact gcggtgaagg ttgcatcgcc ggaagcaagt gaggtgtagc
    56641 caacgttgta atccacttcg ctgggtttat tgacggtata acctaatttc tccagcgcac
    56701 gactgaccag cagtgtctgg aaagtttctt cggtgatggt gctttgaact ggattaacag
    56761 taatgccttt gcccggcagg tcggcagcaa aagtttgtgt agagataagc gtggcaaacg
    56821 ctgtcgcaaa aagtacgcta tgtcgcattg ttattccttt ttttggcagg gtgatatgcc
    56881 cggcgtcacc gggcaagtgc agagttactt aatgaatggg cgggtcagca gaccgacagg
    56941 gccagtggtg taccagcgac gattgccgcg actgcgtgag tcgcgcccaa cggcctgcgt
    57001 caggcggtcg aggataatgg cgaggatcac aatcccgacg ccgccaacgg tggcaagccc
    57061 catatccaga cgaccgatac cgcgaagtac catctgaccc agcccgccga cggcaatcat
    57121 cgaggcgatg accaccatag aaagggccag catcagcgtc tggttaacgc ccgccataat
    57181 ggttggcatc gccagcggta actgaacttt gaacagcatc tggctcgggc tggcaccgaa
    57241 tgagcgcgag gcttcaatca gatccgccgg aacctggtta attcccagaa tcgtcagacg
    57301 gataatcggc ggcagcgcaa agatgatcgt caccaccacg cctggcacgt tgccgatacc
    57361 aaacagcatg acgattggca ccagataaac aaacgcgggc gtggtctgca tggcatcaag
    57421 cagtggacga ataattttcg ccgctcgcgg acttctcgcc agccagatcc ccaacggcaa
    57481 acctatgacg atacagaaca gcagggctgt taacaccagc gccagggtaa ccattgcctg
    57541 tgaccaggca ccgattgcgc cgatggcaat cagcgaaacc agcgtcgcca cgcccattcc
    57601 gaccccggaa atctgccagg cgatgagagc gaaaacgata atcgccaccg gcgcgggcat
    57661 acccagcagc aattgctgga aaccgttgag gatgtaatca accggaacgc gcacgccctg
    57721 aaagacggga cgaaaatggg taacgaccca gtcgatccct tcagtgaccc aactgtcgag
    57781 cgggatcagc gttttatgga acggatcgag aatattaaaa tgctcgacgt ttggcgcagg
    57841 cgtactggtc agccagtcag cgccaccgcc gtcagtcggt gcagtcgccg gtgtacccca
    57901 ggcgtctgcg gattgtgcag cactgtccgc cgctggcgtg gtatcccacg gattattttg
    57961 atcagccatt atttaccccc tcacgatcta aagcgcgcag tagcattcct ttcgaaatga
    58021 tgccgacata ctgttggtcc tcgtcgacca cgggcaccgc acagggtgcc tgtccgacat
    58081 gagagagcaa ttcgctaaga ggcgtttgtg catcgaccgc taacggcgca tcaatcagcg
    58141 ccgcatcaag accttgctgc tgcgttaatg cggctttaag cgaatcgata gacacagcgc
    58201 cgacatactt attaccgcgt tcgatgacgt agccatattc gcgatcttca tcctgcaata
    58261 atttcagtgc cgaacgcggg ccgaagccgg gggttttacg aattaagccg ttcggtgtac
    58321 gacgggcaat atctttcgca ctgaatacct gactaatatc gacgccacgg aagaaggtac
    58381 ggacataatc attcgccgga ttattgagaa tttcatccgg tgtgccgacc tgtaccactt
    58441 caccattttg cataatggca attcggtcgc caatacgcat ggcttcatcg agatcgtggg
    58501 aaataaagac aatggtgcgc tgatgtttcg cctgtaattt taccagctca tcctgcatct
    58561 cggtgcgaat taatggatcg agtgctgaga aggcttcgtc cattaataat atatccggat
    58621 taatcgctaa cgcgcgggct aatccaacac gttgacgcat cccgccagag agttcatccg
    58681 ggtagctgtg ggcataattt tctagcccga cctgacgcag tgcatcaagg gctttttccc
    58741 ggcgttcttc ggcattaatt ccggccaatt ccataccgaa tgcagtattg tccagcacgg
    58801 tcatatgcgg cattaaggca aaggactgga agaccatcgc aatctttttt ctgcgcacct
    58861 cacggagttc ggcgtcggat attttggcaa tatccacacc atcaatcagc acttgcccgc
    58921 gggtgggttc aatcaggcga ttgagaaggc gtaccattgt ggatttaccc gaaccggata
    58981 atcccatgat gacaaatatc tcgccttctt caatggccag actggcgtct tttacgccaa
    59041 gcgatagccc agttttttcc agaatttgtt cttttgaaag tccttgttcg atatatttga
    59101 aagctcgctg tggatgctca ccaaatattt tataaagatt tttaatttct aatttaattg
    59161 ccatgcaata gaaagattcc tgttatttgt ttatgtcgat acattaccca atggaaatag
    59221 tcactttttt ctaccctaac atactgagaa tctgaggcaa cccctgatgg caaatgtggc
    59281 atgaatattg atgtgagcaa atcgccggaa tttcccatga tataagggct gagagcaaat
    59341 cgagaaaagt tgatattttt tgtcaaagat agcgaaaaaa cgcctgaata ttgtggtttc
    59401 aggcgaataa tgcaacagag tgtcagtgta gatcaccaca aaatatttgc gtgataaaaa
    59461 gctatttggc gggaattatt tccctgctgc gggtagtgat atttttgaaa aataacaccc
    59521 tcagaaattc cagtcttcat cttctgtttc gaccgctttt cccatcacat atgaagagcc
    59581 tgaaccggaa aagaaatcgt gattttcatc ggcattcggc gaaagcgcgg cgaggattgc
    59641 cggattcact tccgccattt ctgcgggaaa taacggttcg tagcccagat tcatcaacgc
    59701 cttattggcg ttgtaacaga gaaacgcttt cacatcgtca gcccacgggg tttcggcgta
    59761 cagctcatcg gtgtattgca actcgttgtc ataaagttcc agcaacaaat cgaaggcgaa
    59821 actcttcaac tcttcacgtt gtgcctggga gatcttttcc atgtttttct gatatttata
    59881 gccgatgtaa taaccgtgga ctgcttcatc gcggataatc agacggatca ggtctgcggt
    59941 attggttagc tttccgcggc tggaaaaata catcggcagc cagaaaccgg aatagaacaa
    60001 aaaagattca agaaacacac tggcgatttt ctttttcagc ggatcatccc cgcgataatg
    60061 ttgctgaata atctgagctt ttcgctgcaa cggtgcgttt tcttcactcc aggcgtaggc
    60121 ggcatcgaca tctttggtct ggcacagcgt cgagaaaatc gaactgtaag agcgggcatg
    60181 aaccgcttcc ataaagctga tattcgataa taccgcttct tcatgaggcg tgagtgcatc
    60241 gggcaacagc gaaggcgcgc cgataacatt ttgcagcgtg tcgagcagcg tcaggccagt
    60301 aaaaacgcgc atcgtcagtt gttgttctac gacacttaat gtctgccatg caggaatatc
    60361 gttcgacagc ggcacctttt ctggtagcca gaaattgctg gtcaggcgat tccacacctc
    60421 cagatcttta tcgtcagata tcttgttcca gttgatggcg ctgatacgag agagtttcat
    60481 agatattcct taaagtgcac acgagacgca gccttcaatt tcagtgcctt ccagcgccat
    60541 ctgacgcagg cggatgtaat agagcgtttt gatacctttg cgccaggcgt aaatctgcgc
    60601 tttgttgata tcgcgagtgg tggcggtatc ggggaaaaac agcgtcagcg acagcccctg
    60661 atcgacatgg cgagtcgctt ccgcgtaggt gtcgatgatc ttttctgcgc caatttcgta
    60721 agcgtcctga tacagcgcca gattctcgtt agtcataaac ggggcagggt agtaaacgcg
    60781 tcctgttttg ccctctttgc gtatctctac tttcgccaca atcggatgaa tactcgacgt
    60841 ggcatggttg atataagaga tagaaccggt tggcggcacc gcctggagat tctggttgta
    60901 gataccatag cgcatcacgt cgtcgcgcag ctgtgcccac atctcacggg taggtaacgt
    60961 aataccgcta cgggcaaaca gttcgccaac tttcgccgtt ttcggctgcc agttaccttg
    61021 cagatattgg ctgaaatatt caccactggc atagcgtgac tgtttgaacc cggcgaaggt
    61081 ttcaccgcgt tcgcgcgcca gcaacatcga ggtacgcagt gcgtgccagg tgatggtata
    61141 gaaatagaga ttggtgaaat ccagtgcttc cggcgaccca taagcgatgc cttctcgtgc
    61201 cagataaccg tgtaaattca tctgccccag accgatggcg tgtgaggcgg catttccggc
    61261 ttcgatggac ggcacgctgc ggatatgact catatctgac accgccgtta aaccgcgtac
    61321 ggcagtctct accgtgcggg caaagtcggg ggaatccatg gtgtgggcaa tattcaacga
    61381 ccctagattg caggaaatat catgtcctgt acgagcatag tcgagattct cgtcatattc
    61441 tgaggcgctg ttaacctgca aaatttctga gcagagattg ctcatattta tgcgcccggc
    61501 gatagggtta gcacggttaa ccgtgtcttc atacatgatg tacggatagc cggactcaaa
    61561 ctggatttct gccagtcgct ggaagaaatc acgggcgttg aggtattttt tgcggatgcg
    61621 ttcatcggca accagttcgt catagtgttc actgatggcg acatcggcaa atggcttgcc
    61681 ataaactcgc tctacgtcat aaggcgaaaa cagtgccatc tgcgcattct cttttgccag
    61741 atggaaagtg atatccggga tcaccacgcc cagcgacagt gttttaatgc ggattttttc
    61801 gtcggcattt tcccgttttg tatcgagaaa acgcagaata tcgggatgat gagcatgtaa
    61861 atagactgcc ccagccccct gacgcgcgcc gagttggttg gcataggaaa atgcgtcttc
    61921 cagcattttc atcaccggaa tcacgccaga agattgattt tcaatacgtt taattggcgc
    61981 gcccgcttcc cgcagattcg acagcaaaaa tgctacgccg ccgccgcgtt tcgacagttg
    62041 tagtgcggaa ttcactgccc ggccaatcga ctccatattg tcttcaatac gcagcagaaa
    62101 acaggaaacc agttcgccgc gctgctgctt accgcagttg aggaatgttg gcgtggctgg
    62161 ctgaaagcgt cctgacagca tctcatcagt gagctgcgtc gccagtgttt catcgccttg
    62221 tgccagcgtc agcgccacca tcgtgacgcg atcggcaaaa tcttccagat aacgcttacc
    62281 gtcgaatgtc ttcaacgtat agctggtgta aaacttccat gctcctagga atgtctggaa
    62341 acgaaaaccg ctggcgtgcg cgtgggcaaa cagcgtaatg acaaaatcgc gagagtagcg
    62401 attaaggaca ctttcatcat agtaaccttc gttaaccagc cagttcaggc gctgctgctg
    62461 gctactgaag gccacactgt tcggacgcac atgcgtcgtc ataaaggcgt ctactgcctg
    62521 gcgatcttta tcgaactgaa tgcgacctgc gctatcgtaa aggttaagca tcgcattcag
    62581 agcgtgataa tccatcgttt cctgcgtcag gcgttctgcg gttgtcgttg ccaaaattcg
    62641 gttactcctt tacgaacgtt ttcgatatcg ctttgcgtac ccatgagttc aaagcggtac
    62701 agccacggca cgccgcattt ccgggcaatc acatctccgg cgcggccata tgcctcacca
    62761 aagttgcgat tacccgaagc aataacgccg cgcagcaacg cccggttatg ctcgtcgttt
    62821 aaaaagcgaa tgacctgtcg tggcaccgcg ccagccgtac cgccgccgcc gtaagagggc
    62881 acgatcagga tgtaaggctc gtctacctga atccgttccc gctcattgag cgggatgcgc
    62941 accgcgggca gccctaaacg ttcgataaaa cgctgcgtat tttcggagct gctggaaaag
    63001 tagacgagct ggctcatgca ctggccgcgt gtggcgctgg atgcagacga ttgatcatgt
    63061 ccgggcggaa accagaccag ctaagatcgc ctgcaatcac aaccggcaac tgacgaaagc
    63121 cctgagcacg cagcgcttct gccgcttcag gaacgcgatc gacattaatc atttcaaaat
    63181 caaagccccg gttttccatc gcccgtttgg tggcgtggca ctgaacgcaa tcgttacgag
    63241 tgtaaatagt aatgcgcatg attcgtattt ccgtttaaaa tgaagatacg gcgcgatgat
    63301 acgcgtcggg ttgtctctct gttgatacag agatactaga tgtagttgaa aaaagattca
    63361 accacacaag atatgggaat tttagattga ttacgccacc gatgaatggc tcaacggggt
    63421 aggggatttt gtgttttttt gccgggcgaa tacccggcat caagaagtta catccgcatg
    63481 gaaacggcca gacggttaaa acaattcatc agcccgatgg caaacgttaa gtcgctgatc
    63541 tctgctgcgc tgaaatgctc aagcaaaggc tgataaacct cgtcttccgc atgggttctg
    63601 gcaatttcgg ttaccgattc cgcccatgcc agcgccgccc gctcgcgttc atcaaaatga
    63661 tggcttacgc gccaacctgc cagggcgtcc agtttgtgct gtggcacgcc ggatttgcgc
    63721 aatgctttgc tgtgcatctc cagacaaaat gcgcagccgt tgatttgcga gacgcgcaaa
    63781 taaactaact ccatcaacgt ggtatccagc gtgctatttt ccagtgccgt tttggcctgc
    63841 actagtgcgt tatacactgc cgggctaagt tcgtagtaag gctgacgtaa tgtagtcata
    63901 cagtttctcc tcttattaac gcccgcaatg tagcactata atggactgtt aaagagagcc
    63961 ataaattacc taaaaaagca gaccagatgt ggaaattttg cctgtgccgg ttgatgacaa
    64021 tggactggat atcacaagcg gaatacaaaa ttatcctgat gcgcgttttg ccctgataac
    64081 accagcacac caaagtccgc tgggtgtggc gctctcttta gcgcgcaggc atcagatact
    64141 ggaatgggca gatcgtagtc aggcatggat tattgaagat gattatgaca gtgagtttcg
    64201 ctatcacggt aagccgttac cggcgttaaa aagtctcgac gcaccgcagc gggtaattta
    64261 tgccggaaca ttcagcaaag cgctatttcc tgcattgcgc tgtgcgtggc tggtggtgcc
    64321 ggtgaagcaa attgcacaat tccgccacca ggcgtcactg gctccatgtg cggtacctgt
    64381 tctatggcag aacacactgg cagactttct tcgcgagggg catttctggc ggcatctgaa
    64441 gaaaatgcgt cagcattatg ctcaacgtcg gcaatggatt gagcaagcgt tgacgcagca
    64501 aggatttcag gttgtgccgc agaaaggtgg tatccagatg gtgatcagaa tgataggcga
    64561 tgatattgcc catgcgcgta aagccaatgc tgcaggcctt gcggtgcagg cacttagcga
    64621 ctggcgtatc cgttcaagtg gggaaggtgg attactgctt tcgtttacta atatcgttaa
    64681 cgaaggtatg gcgcgacagg tagcacaaca attacgtaaa gccttaagct aaaaaacccc
    64741 gaagccaggc ttcggggtca aagacgcgta tttattatca tttttgcact acgatttgcg
    64801 catgcttaac agtgcgccga taaaaatacc caccgctgcc gctgtaccca cactacacca
    64861 gggtctttca cgaacaaaag aatccgcgca gccaacggca tcgcgcgcgg cttgctggac
    64921 gcgagtacga ccatgcattc gcgccctggt ttctttcagt agagcctgcg ctttactacg
    64981 agcggcttcg gcttcccctt tggcgtcgct gccccaggac ttcaatacag attcgagact
    65041 gtcagctaat tgattgacat cattctggat atcctgcacg ccatcatcaa cgtcgttgcg
    65101 gttcggtcgg ttaaacatat gatcccccat tagaattcga tgtaattttc agtgtagacc
    65161 ataattttct taactgaggt tcatcgcggc tttatggact tttctgaaag cgttgtgaat
    65221 gcgcaatcag gtaaggtagg cccgcggttg ataataacga ggatcaaata tgtatttacg
    65281 accagacgag gtggcgcgcg tacttgaaaa agtcggtttt actgtcgatg tggtaaccca
    65341 gaaagcgtat ggttaccgcc gtggggaaaa ttatgtctat gttaatcgcg aagcgcgaat
    65401 gggacgtacc gccctggtga ttcatccaac attaaaagaa cgtagttcaa cactggctga
    65461 acccgcttcc gatattaaaa cttgcgatca ttaccagcaa tttccgctct atttagcggg
    65521 tgaacgtcat gagcattacg gtatcccgca tggctttagt tcgcgtgttg cgcttgaacg
    65581 ttatttgaat ggtttatttg gcgaagccag ttaaagacgc gactggcgat gccagtcgcg
    65641 aaaagaaggg tcaggctttt acctgctggt aacggctgac tttaaacagt cggcggcaat
    65701 aatcgaggaa gtagccataa accgccccca tcaacatcga aacaacgatg tttgaactga
    65761 ctgccgccat aatctgatgc cagtctgcgc ccaccactaa caagatcgcc acatacaccg
    65821 gtgactggaa cgtcacataa gccaggatat ccgccagatt ttttatccag cccgacgggc
    65881 taactttgcg tgccgcgcgc ataaacagat cgcggtacat accgtatggc catgcaatta
    65941 agatgttcac cggaatcgct accaatctgg aataaaaaga ctgttcgaag ctcattccgg
    66001 agaggaaaac ttcaatacac atgttcacga cagaacagta aacaaccatc gcgaacgtat
    66061 ctgcaactgc atgacgcaag cgtgactgcg gtgagaacat gctaatgctc cttaataaaa
    66121 gcgtaatggg atgttaatgg agatgaaagc agcggatctc attgccatct gcattttcgc
    66181 atagcgtata tcgctggcta tgactaatca attagtgaaa aatttttatt taatctgttt
    66241 ttatagataa aatattccgt aacggcgcgt tttttgtaca ttgtggattt ttacatgccg
    66301 gtatgattta tttttaatta aatcaattgg atatgtgttt acctttgttt tcactcatga
    66361 aggtttgggt gtgaggtaac
//
LOCUS       DAZFJL010000006        64820 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFJL010000006 DAZFJL010000000
VERSION     DAZFJL010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.006"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..446)
                     /gene="folK"
                     /locus_tag="ACHJ8C_000393"
     CDS             complement(<1..446)
                     /gene="folK"
                     /locus_tag="ACHJ8C_000393"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO5550308.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALKTTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGLSLVEAL"
     gene            complement(443..1840)
                     /gene="pcnB"
                     /locus_tag="ACHJ8C_000394"
     CDS             complement(443..1840)
                     /gene="pcnB"
                     /locus_tag="ACHJ8C_000394"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO5550309.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1900..2796)
                     /gene="gluQRS"
                     /locus_tag="ACHJ8C_000395"
     CDS             complement(1900..2796)
                     /gene="gluQRS"
                     /locus_tag="ACHJ8C_000395"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO5550310.1"
                     /translation="MTDTHYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAEAILRQLEHYGLYWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRDLHHGPDNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALHFLGQQVETHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            complement(2863..3318)
                     /gene="dksA"
                     /locus_tag="ACHJ8C_000396"
     CDS             complement(2863..3318)
                     /gene="dksA"
                     /locus_tag="ACHJ8C_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO5550311.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3496..4200)
                     /gene="sfsA"
                     /locus_tag="ACHJ8C_000397"
     CDS             complement(3496..4200)
                     /gene="sfsA"
                     /locus_tag="ACHJ8C_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO5550312.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAIICVNTLWANRLTKEAILNESISELA
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(4215..4745)
                     /gene="thpR"
                     /locus_tag="ACHJ8C_000398"
     CDS             complement(4215..4745)
                     /gene="thpR"
                     /locus_tag="ACHJ8C_000398"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO5550313.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4819..7248
                     /gene="hrpB"
                     /locus_tag="ACHJ8C_000399"
     CDS             4819..7248
                     /gene="hrpB"
                     /locus_tag="ACHJ8C_000399"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO5550314.1"
                     /translation="MSSLPVAAVLPELLAALDGASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLTSRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCGDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALDGERLSAQGQKMAALGDDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLARAYADRIARRRGQDE
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLMVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWVMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEASN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7342..9876
                     /gene="mrcB"
                     /locus_tag="ACHJ8C_000400"
     CDS             7342..9876
                     /gene="mrcB"
                     /locus_tag="ACHJ8C_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO5550315.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLNPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10096..12339
                     /gene="fhuA"
                     /locus_tag="ACHJ8C_000401"
     CDS             10096..12339
                     /gene="fhuA"
                     /locus_tag="ACHJ8C_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO5550316.1"
                     /translation="MAPSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLDLYNPVNTDFDFNAKDP
                     DNSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12390..13187
                     /gene="fhuC"
                     /locus_tag="ACHJ8C_000402"
     CDS             12390..13187
                     /gene="fhuC"
                     /locus_tag="ACHJ8C_000402"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO5550317.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13187..14077
                     /gene="fhuD"
                     /locus_tag="ACHJ8C_000403"
     CDS             13187..14077
                     /gene="fhuD"
                     /locus_tag="ACHJ8C_000403"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO5550318.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAVIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            14074..16056
                     /gene="fhuB"
                     /locus_tag="ACHJ8C_000404"
     CDS             14074..16056
                     /gene="fhuB"
                     /locus_tag="ACHJ8C_000404"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO5550319.1"
                     /translation="MSKRIALFPVLLLALLVVAAAALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16091..17371)
                     /gene="hemL"
                     /locus_tag="ACHJ8C_000405"
     CDS             complement(16091..17371)
                     /gene="hemL"
                     /locus_tag="ACHJ8C_000405"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO5550320.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LREAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17537..17584
                     /gene="yadX"
                     /locus_tag="ACHJ8C_000406"
     CDS             17537..17584
                     /gene="yadX"
                     /locus_tag="ACHJ8C_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO5550321.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17596..19017
                     /gene="clcA"
                     /locus_tag="ACHJ8C_000407"
     CDS             17596..19017
                     /gene="clcA"
                     /locus_tag="ACHJ8C_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO5550322.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASARENT"
     gene            19041..19106
                     /gene="yadW"
                     /locus_tag="ACHJ8C_000408"
     CDS             19041..19106
                     /gene="yadW"
                     /locus_tag="ACHJ8C_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO5550323.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19099..19443
                     /gene="erpA"
                     /locus_tag="ACHJ8C_000409"
     CDS             19099..19443
                     /gene="erpA"
                     /locus_tag="ACHJ8C_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO5550324.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19490..20113)
                     /gene="yadS"
                     /locus_tag="ACHJ8C_000410"
     CDS             complement(19490..20113)
                     /gene="yadS"
                     /locus_tag="ACHJ8C_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO5550325.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20151..20951)
                     /gene="btuF"
                     /locus_tag="ACHJ8C_000411"
     CDS             complement(20151..20951)
                     /gene="btuF"
                     /locus_tag="ACHJ8C_000411"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO5550326.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20944..21642)
                     /gene="mtnN"
                     /locus_tag="ACHJ8C_000412"
     CDS             complement(20944..21642)
                     /gene="mtnN"
                     /locus_tag="ACHJ8C_000412"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO5550327.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCKIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21726..23243
                     /gene="dgt"
                     /locus_tag="ACHJ8C_000413"
     CDS             21726..23243
                     /gene="dgt"
                     /locus_tag="ACHJ8C_000413"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO5550328.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNALRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLNLPLSDFTELVEKERVKRFPIETRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23373..24797
                     /gene="degP"
                     /locus_tag="ACHJ8C_000414"
     CDS             23373..24797
                     /gene="degP"
                     /locus_tag="ACHJ8C_000414"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO5550329.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGLGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSDLAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSST
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            24952..26109
                     /gene="cdaR"
                     /locus_tag="ACHJ8C_000415"
     CDS             24952..26109
                     /gene="cdaR"
                     /locus_tag="ACHJ8C_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO5550330.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26162..26548)
                     /gene="yaeH"
                     /locus_tag="ACHJ8C_000416"
     CDS             complement(26162..26548)
                     /gene="yaeH"
                     /locus_tag="ACHJ8C_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO5550331.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26858..27682)
                     /gene="dapD"
                     /locus_tag="ACHJ8C_000417"
     CDS             complement(26858..27682)
                     /gene="dapD"
                     /locus_tag="ACHJ8C_000417"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO5550332.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFANYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27713..30385)
                     /gene="glnD"
                     /locus_tag="ACHJ8C_000418"
     CDS             complement(27713..30385)
                     /gene="glnD"
                     /locus_tag="ACHJ8C_000418"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO5550333.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCAWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRHLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDLWPRLPSPELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHHIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKSLVLLSPHATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDDMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30447..31241)
                     /gene="map"
                     /locus_tag="ACHJ8C_000419"
     CDS             complement(30447..31241)
                     /gene="map"
                     /locus_tag="ACHJ8C_000419"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO5550334.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31609..32334
                     /gene="rpsB"
                     /locus_tag="ACHJ8C_000420"
     CDS             31609..32334
                     /gene="rpsB"
                     /locus_tag="ACHJ8C_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO5550335.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32469..33320
                     /gene="tsf"
                     /locus_tag="ACHJ8C_000421"
     CDS             32469..33320
                     /gene="tsf"
                     /locus_tag="ACHJ8C_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO5550336.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33467..34192
                     /gene="pyrH"
                     /locus_tag="ACHJ8C_000422"
     CDS             33467..34192
                     /gene="pyrH"
                     /locus_tag="ACHJ8C_000422"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO5550337.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34342..34899
                     /gene="frr"
                     /locus_tag="ACHJ8C_000423"
     CDS             34342..34899
                     /gene="frr"
                     /locus_tag="ACHJ8C_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO5550338.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            34991..36187
                     /gene="ispC"
                     /locus_tag="ACHJ8C_000424"
     CDS             34991..36187
                     /gene="ispC"
                     /locus_tag="ACHJ8C_000424"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO5550339.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKMMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36376..37134
                     /gene="ispU"
                     /locus_tag="ACHJ8C_000425"
                     /gene_synonym="uppS"
     CDS             36376..37134
                     /gene="ispU"
                     /locus_tag="ACHJ8C_000425"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO5550340.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37147..38004
                     /gene="cdsA"
                     /locus_tag="ACHJ8C_000426"
     CDS             37147..38004
                     /gene="cdsA"
                     /locus_tag="ACHJ8C_000426"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO5550341.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRIIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWVNLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38016..39368
                     /gene="rseP"
                     /locus_tag="ACHJ8C_000427"
     CDS             38016..39368
                     /gene="rseP"
                     /locus_tag="ACHJ8C_000427"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO5550342.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGKGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39398..41830
                     /gene="bamA"
                     /locus_tag="ACHJ8C_000428"
     CDS             39398..41830
                     /gene="bamA"
                     /locus_tag="ACHJ8C_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO5550343.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            41952..42437
                     /gene="skp"
                     /locus_tag="ACHJ8C_000429"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             41952..42437
                     /gene="skp"
                     /locus_tag="ACHJ8C_000429"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO5550344.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42441..43466
                     /gene="lpxD"
                     /locus_tag="ACHJ8C_000430"
     CDS             42441..43466
                     /gene="lpxD"
                     /locus_tag="ACHJ8C_000430"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO5550345.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43571..44026
                     /gene="fabZ"
                     /locus_tag="ACHJ8C_000431"
     CDS             43571..44026
                     /gene="fabZ"
                     /locus_tag="ACHJ8C_000431"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO5550346.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44030..44818
                     /gene="lpxA"
                     /locus_tag="ACHJ8C_000432"
     CDS             44030..44818
                     /gene="lpxA"
                     /locus_tag="ACHJ8C_000432"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO5550347.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGETTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            44818..45966
                     /gene="lpxB"
                     /locus_tag="ACHJ8C_000433"
     CDS             44818..45966
                     /gene="lpxB"
                     /locus_tag="ACHJ8C_000433"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO5550348.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            45963..46559
                     /gene="rnhB"
                     /locus_tag="ACHJ8C_000434"
     CDS             45963..46559
                     /gene="rnhB"
                     /locus_tag="ACHJ8C_000434"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO5550349.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            46596..50078
                     /gene="dnaE"
                     /locus_tag="ACHJ8C_000435"
     CDS             46596..50078
                     /gene="dnaE"
                     /locus_tag="ACHJ8C_000435"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO5550350.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGTAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLDTELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50091..51050
                     /gene="accA"
                     /locus_tag="ACHJ8C_000436"
     CDS             50091..51050
                     /gene="accA"
                     /locus_tag="ACHJ8C_000436"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO5550351.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLTDLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51148..53289
                     /gene="ldcC"
                     /locus_tag="ACHJ8C_000437"
     CDS             51148..53289
                     /gene="ldcC"
                     /locus_tag="ACHJ8C_000437"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO5550352.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RGEFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFSDADANHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRSMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTEESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53346..53735
                     /gene="yaeR"
                     /locus_tag="ACHJ8C_000438"
     CDS             53346..53735
                     /gene="yaeR"
                     /locus_tag="ACHJ8C_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO5550353.1"
                     /translation="MLGLKQVHHIAIIATDYAMSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            53800..55113
                     /gene="tilS"
                     /locus_tag="ACHJ8C_000439"
     CDS             53800..55113
                     /gene="tilS"
                     /locus_tag="ACHJ8C_000439"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO5550354.1"
                     /translation="MTLTLNTQLLSSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVKHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWARQYDLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTQQGTLKIAPMLAMSDARRAAIIRRWLAGQNAPMPSREALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVAGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAVGENGVSFVWQKTLSQVKAG"
     gene            complement(55147..55407)
                     /gene="rof"
                     /locus_tag="ACHJ8C_000440"
     CDS             complement(55147..55407)
                     /gene="rof"
                     /locus_tag="ACHJ8C_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO5550355.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGATRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55394..55594)
                     /gene="yaeP"
                     /locus_tag="ACHJ8C_000441"
     CDS             complement(55394..55594)
                     /gene="yaeP"
                     /locus_tag="ACHJ8C_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO5550356.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDTLS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            55760..56305
                     /gene="yaeQ"
                     /locus_tag="ACHJ8C_000442"
     CDS             55760..56305
                     /gene="yaeQ"
                     /locus_tag="ACHJ8C_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO5550357.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFSNLSIWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56302..56724
                     /gene="arfB"
                     /locus_tag="ACHJ8C_000443"
     CDS             56302..56724
                     /gene="arfB"
                     /locus_tag="ACHJ8C_000443"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO5550358.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            56738..57448
                     /gene="nlpE"
                     /locus_tag="ACHJ8C_000444"
                     /gene_synonym="cutF"
     CDS             56738..57448
                     /gene="nlpE"
                     /locus_tag="ACHJ8C_000444"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414734.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO5550359.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEGNPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(57478..58302)
                     /gene="yaeF"
                     /locus_tag="ACHJ8C_000445"
     CDS             complement(57478..58302)
                     /gene="yaeF"
                     /locus_tag="ACHJ8C_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFO5550360.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATVVNAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQVCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDISAFKPETQLQYVGHMKPGIYIKAGRFVGLTQ"
     gene            complement(58355..60073)
                     /gene="proS"
                     /locus_tag="ACHJ8C_000446"
     CDS             complement(58355..60073)
                     /gene="proS"
                     /locus_tag="ACHJ8C_000446"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO5550361.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPSELWQESGRWEQYGPELLRIADRGDR
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVDYL
                     VKQIKG"
     gene            complement(60184..60891)
                     /gene="tsaA"
                     /locus_tag="ACHJ8C_000447"
     CDS             complement(60184..60891)
                     /gene="tsaA"
                     /locus_tag="ACHJ8C_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO5550362.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPNASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(60888..61292)
                     /gene="rcsF"
                     /locus_tag="ACHJ8C_000448"
     CDS             complement(60888..61292)
                     /gene="rcsF"
                     /locus_tag="ACHJ8C_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO5550363.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAESAKPKVP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61410..62225)
                     /gene="metQ"
                     /locus_tag="ACHJ8C_000449"
     CDS             complement(61410..62225)
                     /gene="metQ"
                     /locus_tag="ACHJ8C_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO5550364.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62265..62918)
                     /gene="metI"
                     /locus_tag="ACHJ8C_000450"
     CDS             complement(62265..62918)
                     /gene="metI"
                     /locus_tag="ACHJ8C_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO5550365.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(62911..63942)
                     /gene="metN"
                     /locus_tag="ACHJ8C_000451"
     CDS             complement(62911..63942)
                     /gene="metN"
                     /locus_tag="ACHJ8C_000451"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO5550366.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAITWLQEHH
                     VKVEVLGYV"
     gene            64130..64702
                     /gene="gmhB"
                     /locus_tag="ACHJ8C_000452"
     CDS             64130..64702
                     /gene="gmhB"
                     /locus_tag="ACHJ8C_000452"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO5550367.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAETAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15258 a        16107 c        17879 g        15576 t
ORIGIN      
        1 tgtagagctt caactaatga taagccatca ggaaatacca attccggcgc aatttcaaac
       61 agtggccaca gcataaagcc gcgatttttc atatcgtagt gcggaacggt cagacgttcg
      121 gtatttatta cttcattacc aaacagcatg atgtcgagat ccagcgtgcg cggtccccag
      181 cgttcagctt tacggacgcg accttgctgc aattcgatac gctgtgtgtg attgagtagc
      241 tcttcagggg caagagtggt tttcagcgcg acggctgcat ttaagtaatc gggttgatct
      301 tgcggcccca gcggtggggt gcggtaaaac gaagaaacgg caagaatgcg gctttctgga
      361 atatcgccta atgctttcag ggcagcattg acctgctcca gcggagaggc cagattgctg
      421 cctatggcaa tatacgccac tgtcatgcgg taccctcacg acgcggtgcg cgtttgcgtg
      481 gacgacgagt acgacgacgc ggtgacggtt cttcatccag ttcgttaagc atcccttttt
      541 ggtctggtgg cgcggaaacc tggaactcac cccaccattt caccagacgt tgcagttcag
      601 cgttacgctc aacttcggct cgtaaggcca acaggtcgta tgccgcacgg aacttaggat
      661 gctccagcag tttccatgcg cgtttaccct gacgacggga catacgcaac tgcaactgcc
      721 agatatcgcg ggttagcgtg gtcagccgtt tcgggatcgc cagtgaacgg caggcttcgt
      781 ccagcacgtc gttcatcgcc agtgcgaaag cgtcgtgata ggtcaggccg ctttcctggg
      841 cgatcttctg tgccgtctcc agcaatgggt accagaacat ggcggcaaac aggaacgccg
      901 ggttcacgcg catatcgtta tggatacgcg tatcggtatt cttcagtacc tgctcaataa
      961 tgcgttccat cgggctgtcg ccattttccg tgaagtagcg ggtaatggtc gggaacagcg
     1021 gctggaacag atgatattca cacagcagct tataggtttc gtaaccgtag cccgcttgca
     1081 acagtttcag cgattcttca aacagacgtg ccggtgggat atcgttcagt aaggttgcca
     1141 ggcgtgggat cggctcggcg gtttccgggc tgatgcgcat gcccaatttg gcggcaaaac
     1201 gaaccgcgcg gagcatacgt accggatctt cacggtagcg cgtttccggg ttaccaatca
     1261 gacggataac gccgtctttc agatccttca tgccgccaac gtaatcacgg acggtaaaat
     1321 ctgccacgct gtaatacagg ctgttgatag tgaaatcgcg gcgctgggcg tcttcttcga
     1381 tggagccgaa aatgttgtcg cgcagcaaca taccgttttg cccgcgttgg gaggtcgtgc
     1441 ggtcgctgac gttaccttcg tgatgtccac ggaaggtcgc aacttcgata atctccgggc
     1501 caaacatcac atgagccaga cggaaacggc gacccaccag gcggcagtta cggaacagtt
     1561 tgcgcacctg ctctggcgtg gcgttggtgg tcacgtcaaa atctttcggc tttttgccaa
     1621 gtaacaggtc gcgcacgccg ccgccaacca gccaggcttc gtatcccgct ttattgagcc
     1681 tgtacattac cttcagggca ttttcactga tatctttgcg ggaaatagca tgctgctcac
     1741 gcgggatcac cgtcacctgt ggacgggcga ctgcctgttc agcctcgctt tcctcgcggc
     1801 ttagcacctt gcggcaaaaa ttagcgactc gggtaaaaat agtacacctc ggtagtgtca
     1861 aacatcattc aggacaaaaa aatagcggct aatcatagct cagcatgacg catttgagaa
     1921 tgttgaattt acaattgccg actcaggcac ggcggtcagc gtccagtttt tgacggctga
     1981 ctgaaggatt tgctcgacgc tgaaatcctg ccagtgtgtt tctacctgct gacccagaaa
     2041 atgaagtgcc gcgattaaca ccgggcgcgg atcgcctttc ggcaacgcag gcgcatgatt
     2101 ctgcttggaa agtttagcgc cttgtggatt aagcgccagc ggtaaatgga tgtaatctgg
     2161 cactttccag ccaaaaagct ggtacagcga gatttgccgc accgtcggtt cgatcaaatc
     2221 tgccccgcgg acaatttcgc tcacgccttg gaagtgatca tccaccacca ccgccaggtt
     2281 ataggcaaac aaaccatcac ggcgatgaat aataaaatct tcccgtgcca gtttttcgtc
     2341 ggcgtgaata atgcctcgca gcaggtcagt aaactgggtg accggatgct gctggcggat
     2401 acgtactgcg gcgttgtctg gtccatgatg caaatcccgg caatgaccgt cgtaaatacc
     2461 gccaatgctt tgaatacgcg cacgcgtgca ggtgcagtaa taacttagtc cttgctcatg
     2521 taaccaggcg agtgcttcgc gataggcgtg gtgacgttgc gattgccaga gaacgtcgcc
     2581 gtcccagtac aggccgtaat gttccagctg gcgcaggata gcttctgcgg caccgggaac
     2641 ttcacgaggc gggtcgatat cttcaatgcg taccagccag cgaccttgcc gggcgcgagc
     2701 ctgcaaatag ctgccaagcg cggcaatcag agaaccaaaa tgaagctcgc cggaaggaga
     2761 gggggcgaag cggccaatat agtgtgtgtc tgtcatctct ttgaacaaaa aataaggcgg
     2821 gagcatttcc cgcctgtggt aaacgtgatg gaacggctgt aattagccag ccatctgttt
     2881 ttcgcgaatt tcagccagcg ttttgcagtc gatgcacaga tcggctgtcg ggcgcgcttc
     2941 cagacggcga ataccaattt caacaccgca ggattcgcag tagccgaaat cttcgtcttc
     3001 cacttttttc agcgtcttct cgatcttttt gatcagctta cgctcgcgat cgcggttacg
     3061 cagttcgagg ctgaactctt cttcctgggc tgcacggtct accgggtccg ggaagttggc
     3121 tgcttcatcc tgcatatgtg taacggtgcg atcgacttca tccctgagtt gattacgcca
     3181 tgcttccaga atacgacgga agtgcgccag ctgggcttca ttcatatact cttcgcccgg
     3241 cttctcctga tatggttcca ccccagcgat ggcgagaata ctcagggacg atgttttacg
     3301 gttttgccct tcttgcatgt tgcttctcct taacacgcac tatcgatccc catattcggg
     3361 ggaaaaatga ggccgctata aatagcagat gcttttccgg atagcaatta tctaaacgta
     3421 acacttgaca actgtgtgag gaaaagcgta tttgcgcacg cgaccagaat gtaaattaac
     3481 cagttactta ctctactaca atgtaaccgg tagtgatttt ttaagagcca tgccttcggc
     3541 agaaagttcc gctttgtaag ccagaatttc tacccccctc tgttgagctt ctgacaatag
     3601 ttgcgcgtat ttctcatcga tgtggcgcgc gggtgaaaac cgtgtaatgg ctgaatgcag
     3661 cacggcgaaa aagataaccg cacgctggcc ttcagccgct acgctcatca actcccgaag
     3721 gtgtttctga cctcgttcag tgaccgcatc gggaaaatat ccctgttcgt tctccgctaa
     3781 cgtaaccgat ttcacttcaa tatagcagtc tggacgcgaa tccgcctgca acataaagtc
     3841 aatacggctg cgttcggcgc cgtattttac ttcgcttttc agcgagctat agcctgccag
     3901 ctctgaaatt gattcattaa ggatagcctc tttcgtcaac ctgttagccc aaagcgtatt
     3961 gacgcaaata attgcgccgc tctggctttg agttaattcc caggtgtgtg ggtatttccg
     4021 tttggtgttg tctgaagtcg aataccagac ggtatcgcca ggcgttgcac aaccggtcat
     4081 cgcacccgta ttcgggcagt gtagcgttaa ttcgcgacca tcgggtgtga tcacatcggc
     4141 taaaaaacgt ttgtagcgct gaattagcgt cgcgcgctgt agagggggag aaaattccat
     4201 tgcgacaatc cttgttattg cgttagcgcc cagcgtttta gcggcgtgta gcgtgtgcgt
     4261 ccacgggcaa acgaggaggc gtaaagggtg aactccgtca ccgtatacga ccagttaaaa
     4321 cctggcggcg ggattgtcac cgcctcgctg gcgtcgcgca ataaggtaat atgtggatga
     4381 aacggacgat tgctttgaaa acaaccgctg cgggcagcct gtgaacggag catattcgcc
     4441 agctggatta agccgcgtgg tggctgtcgc atccctaacc acaccacacg cgaacgcagc
     4501 cattgaccgg cgtcatcaag cgtgagtgtg aaaccaggtt gacgaatccg tccggctaaa
     4561 agagaaagcg ccttctcttt ctctgcgctc acttcgccta aaaatgccag agtcagatgc
     4621 aaattatcgg cggcgaccgg acgtcccgcc tcaggtggga agtgtgtggc gcgccagtgg
     4681 ataatctgtt cgcggatttc tgcaggtaag tcgatagcaa agaacagccg ttgcggttca
     4741 gacatgtgag gcactcggtt atgaattacc gcgatgctac aatgtggcgc gaagaatgtt
     4801 aaccctctgg agcgttttgt gtcgtcgttg cccgttgctg ccgtcttacc tgagttactt
     4861 gctgccctcg atggtgcatc gcaggtgtta ttaagtgcgc cgaccggggc cgggaaatca
     4921 acctggctgc cgctgcaact gctggcgcat cccggcatta acgggaaaat tatcctgctg
     4981 gagccgcgtc gtctggcggc gcgtaacgtc gcgcaacgac tggcggagct gcttaacgaa
     5041 aagccaggcg ataccgttgg ctaccggatg cgtgcgcaaa actgcgtcgg gccgaatacc
     5101 cgcctggaag tagttaccga aggcgtgctg acgcggatga tccagcgtga cccggaactg
     5161 agtggtgttg gattggtgat ccttgatgaa tttcatgagc gcagcttgca ggcggatttg
     5221 gcattggcgt tgttactcga tgtgcaacaa ggtctgcgtg atgaccttaa actgctgatt
     5281 atgtcggcta cgctggacaa cgaccgcttg cagcaaatgc tgccagaagc gcccgtcgtc
     5341 atctcagaag ggcgctcgtt tccggttgaa cgccgttatt tgccgctgcc cgctcatcag
     5401 cgttttgacg aagccgtcgc ggttgctacc gccgaaatgc tgcgtcagga aagtggatca
     5461 ttactgttat ttttacctgg cgtcggagaa attcagcgtg tgcaggaaca actgacttca
     5521 cgcatcggca gtgatgtgtt gctctgcccg ctgtatggcg cgttgtcgct gaacgatcag
     5581 cgaaaagcga tcctcccggc accgcaaggg atgcgcaaag tggtgctggc gaccaatatt
     5641 gctgaaacaa gtttaaccat cgaaggtatt cgtctggtgg tggattgtgc ccaggaaagg
     5701 gtggcgcgtt ttgatccgcg tacagggctt acgcggttga ttactcaacg cgttagtcag
     5761 gcgtcgatga cgcaacgtgc cgggcgtgcc gggcgtctgg agccgggtat ctgcctgcat
     5821 ttaatcgcca aagaacaagc agaacgcgct gccgcgcaaa gtgaaccgga gatcttacaa
     5881 agcgatcttt ccggtttgct gatggaatta ctgcaatggg gatgcggcga tccggcgcag
     5941 atgagctggc tggatcaacc gccaacggtg aatctactgg ccgcgaaacg cctgttacag
     6001 atgctggggg cgctggatgg cgaacgccta agcgcgcaag ggcaaaaaat ggcagcgctg
     6061 ggtgacgatc cgcgtttagc ggcaatgctg gttagcgcga agaacgacga cgaagctgct
     6121 accgcggcaa aaattgccgc cattctcgaa gagccgccac ggatgggcaa tagtgacctg
     6181 ggcgtggcgt tttcgcgcaa tcaacccgcc tggcagcaac gtagtcagca actgttaaaa
     6241 cgcttaaacg tacgcggcgg tgaggcagac agttcgctta tcgcgccgct acttgccaga
     6301 gcgtatgccg atcgcattgc tcgtcgccgt gggcaagatg aacgctatca actggcgaac
     6361 ggcatggggg cgatgctcga tgccgacgat gcgctaagcc gccacgaatg gttgatcgca
     6421 ccgttattat tgcagggcag cgcctcgccg gatgcgcgga ttttactggc gctgccggtc
     6481 gatattgatg agttagtaca acgctgcccg cagctggtac agcagtctga cactgtggag
     6541 tgggatgacg cgcaaggtac gctgaaagcc tggcgtcggc tgcaaattgg tcagttgatg
     6601 gttaaagtgc aaccgctggc gaaaccctcg gaagacgagt tgcatcaggc gatgcttaac
     6661 ggcattcgtg ataaaggttt aagcgtgctc aactggacgg cggaagcgga acagctacgc
     6721 ttgcgtttgt tatgcgccgc aaagtggttg ccggaatatg actggccagc ggttgatgat
     6781 gaaagtttgt tggcgacgct ggaaacgtgg ctactgccgc atatgacagg cgtacattcg
     6841 ctacgcggcc tgaaatcact cgatatttat caggcactgc gtggattact tgattgggta
     6901 atgcagcaac gtctggatag tgaattgcct gcgcattaca ctgtgccgac gggaagccgg
     6961 atcgccattc gttaccatga agataacccg cccgcgctgg cggtgagaat gcaggagatg
     7021 tttggtgagg cctctaatcc gactatcgcc caggggcgcg taccgttggt gctggagttg
     7081 ctttcccctg cccaaaggcc gctgcaaatc acgcgtgatt tgagcgcctt ctggaaagga
     7141 gcgtaccgtg aggtgcaaaa agagatgaaa gggcgttatc ccaaacatgt ctggccggac
     7201 gacccggcaa acaccgcacc gacgcgacga acgaaaaagt attcgtgaag accaaagcaa
     7261 atttgagaga tatcttcttc tgtcttgcaa cagaagaaca gaaaatcggg cttttgcgcc
     7321 tgaatattgc ggagaaaaag catggccggg aatgaccgcg agccaattgg acgcaaaggg
     7381 aaaccgacgc gtccggtcaa acaaaaggta agccgtcgtc gttacgaaga tgacgatgat
     7441 tacgacgatt atgatgacta tgaggatgaa gaaccgatgc cgcgcaaagg taagggcaaa
     7501 ggcaaagggc gtaagcctcg tggcaaacgc ggctggctat ggctgctgct aaaactggct
     7561 atcgtttttg ccgtgctgat cgccatttac ggcgtttatc tcgatcaaaa aattcgtagc
     7621 cgtattgatg gcaaggtctg gcaattgccc gcggcagttt atggccgaat ggtcaacctt
     7681 gagccagaca tgaccatcag caagaacgag atggtgaagc tgctggaggc gacccagtat
     7741 cgccaggtgt cgaaaatgac ccgtcctggc gagtttaccg tacaggccaa cagcattgag
     7801 atgatccgcc gtccgtttga tttcccggac agcaaagaag ggcaggtgcg cgcgcgtctg
     7861 acctttgatg gcgatcatct ggcgacgatc gtcaatatgg agaacaaccg tcagttcggt
     7921 ttcttccgtc ttgatccgcg cctgatcacc atgatttctt cgccaaacgg tgagcagcgt
     7981 ctgtttgtgc cgcgcagtgg tttcccggat ttgctggtgg atactttgct ggcgacagaa
     8041 gaccgtcatt tctacgagca tgatgggatc agtctctact caatcggacg tgcggtgctg
     8101 gcaaacctga ctgccggacg cacggtgcag ggggcgagta cgctgacgca acagttggtt
     8161 aaaaacctgt tcctctccag cgagcgttct tactggcgta aagcgaacga agcctacatg
     8221 gcgctgatca tggacgcgcg ttacagcaaa gaccgtattc ttgagctgta tatgaacgag
     8281 gtgtatctcg gtcagagcgg cgacaacgaa atccgcggct tcccgctggc gagcttgtat
     8341 tactttggtc gcccggtaga agaattgagc ctcgatcagc aggcgctgtt ggtgggtatg
     8401 gtgaaagggg cgtccatcta caacccgtgg cgtaacccga aactggcgct ggagcgacgt
     8461 aacctggtac tacgtttgct gcaacagcaa caaattattg atcaagaact ctacgacatg
     8521 ctgagtgcgc gtccgctggg tgttcagccg cgcggtgggg tgatctctcc tcagccagcc
     8581 tttatgcagc tggtgcgtca ggagctgcag gcgaaattag gcgataaggt aaaagatctc
     8641 tccggcgtga agatcttcac tacctttgac tcggtggccc aggacgcggc agaaaaagcc
     8701 gccgtggaag gcattccggc actgaagaaa cagcgtaagt tgagcgatct tgaaaccgcg
     8761 attgtggttg ttgaccgctt tagcggtgaa gttcgtgcga tggtcggtgg ttctgagccg
     8821 cagtttgcgg gctacaaccg tgcgatgcag gcgcgtcgtt cgattggttc ccttgcaaaa
     8881 ccagcgactt atctaacggc cttaagccag ccgaaaatct atcgtttgaa tacgtggatt
     8941 gccgatgcgc caatcgcgct gcgtcagccg aatggccagg tctggtcacc gcagaacgat
     9001 gatcgtcgtt atagcgaaag tggcagggtg atgctggtag atgcgttgac ccgttcgatg
     9061 aacgtgccga cggtgaatct gggcatggcg ttggggctgc ctgcggttac ggagacctgg
     9121 attaaactgg gtgtgccgaa agatcaactg aatccagttc cagcaatgtt gttgggggcg
     9181 ttgaacttaa cgccaatcga agtggcgcag gccttccaga ctatcgccag cggtggtaat
     9241 cgtgctccgc tttccgcgct acgttcagtc atcgcagaag acggcaaggt gttgtatcag
     9301 agcttcccgc aggcggaacg cgctgttccg gcgcaggcgg cgtatctgac actatggacc
     9361 atgcagcagg tggtacaacg cggtacggga cgtcagcttg gggcgaaata tccgaacctg
     9421 catctggcag ggaaaacagg gactaccaac aataacgtag atacctggtt tgcgggcatt
     9481 gatgggagca cggtgaccat cacctgggtc ggccgtgata acaaccagcc gaccaaactg
     9541 tatggtgcca gcggggcaat gtcgatttat cagcgttatc tggctaacca gacgccaacg
     9601 ccgctgaatc ttgttccgcc agaagatatt gcagatatgg gcgtggacta cgacggcaac
     9661 tttgtttgca gcggtggtat gcgtgtcttg ccggtctgga ccagcgatcc gcaatcgctg
     9721 tgccagcaga gtgagatgca gcagcaaccg tcaggcaatc cgtttgatca gtcttctcag
     9781 ccgcagcaac agccgcaaca gcaacctgct cagcaagagc agaaagacag cgacggtgta
     9841 gccggttgga ttaaagatat gtttgggagt aattaacgtc taagcgtgat ataccggatg
     9901 gcgagctgcc atccggtaaa ataacatccc ctccaaagta ttaacccttt cttttcatct
     9961 gattgtttat tgacccttca ggaatgcgca gattgcgtac cgcttgcgaa cccgccagcg
    10021 tttcgaatat tatctcatct ttataataat cattctcgtt tacgttatca ttcactttac
    10081 accagagaaa taccaatggc accttccaaa actgctcagc caaaacactc actgcgtaaa
    10141 atcgcagttg tagtagccac agcggttagc ggcatgtctg tttatgcaca ggcagcggtt
    10201 gaaccgaaag aagacactat caccgttacc gctgcacctg cgccgcaaga aagcgcatgg
    10261 gggccggctg caactattgc ggcgcgacag tcagctaccg gcactaaaac cgatacgccg
    10321 attcaaaaag tacctcaatc tatttctgtc gtcaccgctg aagagatggc gctgcatcag
    10381 cctaagtcgg tgaaagaagc gcttagctat acgccgggtg tctctgttgg tacgcgtggc
    10441 gcatccaaca cctatgacca cctgatcatt cgcggttttg cggcagaagg ccaaagccag
    10501 aataactatc tgaatggcct gaagttgcag ggcaacttct ataacgatgc ggtcatcgac
    10561 ccgtatatgc tggaacgtgc tgaaattatg cgtggcccgg tttccgtgct gtatgggaaa
    10621 agcagcccag gcggcctgtt gaatatggtc agcaagcgtc cgaccaccga accgctgaaa
    10681 gaagttcagt ttaaagccgg tactgacagc ctgttccaga ccggttttga ctttagcgat
    10741 gcgctggatg atgacggcgt ttattcttat cgcctgaccg gtcttgcgcg ttctgccaat
    10801 gcccagcaga aagggtcaga agagcagcgt tatgctattg caccggcgtt cacctggcgt
    10861 ccggatgata aaaccaattt caccttcctt tcttacttcc agaacgagcc ggaaactggt
    10921 tattacggct ggttgccgaa agagggaacc gttgagccgc tgccgaacgg taagcgtctg
    10981 ccgacagact ttaacgaagg ggcgaagaac aacacctatt ctcgtaacga gaagatggtc
    11041 ggctacagct tcgatcacga atttaacgat acctttactg tgcgtcagaa cctgcgcttt
    11101 gctgaaaaca aaacctcgca aaacagcgtt tatggttacg gcgtctgctc cgatccggcg
    11161 aatgcttaca gcaaacagtg tgcggcatta gcgccagcgg ataaaggcca ttatctggca
    11221 cgtaaatacg tcgttgatga tgagaagctg caaaacttct ccgttgatac ccagttgcag
    11281 agcaagtttg ccactggcga tatcgaccac actctgctga ccggtgtcga ctttatgcgt
    11341 atgcgtaatg acatcaacgc ctggtttggt tacgacgact ctgtgccact gctcgatctg
    11401 tacaatccgg taaataccga tttcgacttc aatgcgaaag acccggataa ctccggtccg
    11461 taccgcattc tgaataagca gaagcaaacg ggcgtttatg ttcaggatca ggcgcagtgg
    11521 gataaagtgc tggtcaccct gggcggtcgt tacgactggg cagatcaaga atctcttaac
    11581 cgcgtagcgg gaacgaccga taaacgtgat gacaaacagt ttacctggcg tggtggtgtt
    11641 aactacctgt ttgataatgg tgtaacacca tacttcagct atagcgaatc gtttgaacct
    11701 tcttcgcaag ttgggaagga tggtaatatt ttcgcaccgt ctaaaggtaa gcagtatgaa
    11761 gtcggcgtga aatatgtacc ggaagatcgt ccgattgtag ttactggtgc cgtgtataat
    11821 cttactaaaa ccaacaacct gatggcggac cctgagggtt ccttcttctc ggttgaaggt
    11881 ggcgagatcc gcgctcgtgg cgtagaaatt gaagcgaaag cggcgctgtc ggcgagtgtt
    11941 aacgtagtcg gttcttatac ttacaccgat gcggaataca ctaccgatac tacctacaaa
    12001 ggcaatacgc ctgcacaggt gccaaaacac atggcttcgc tgtgggctga ctataccttc
    12061 tttgacggtc cgctttcagg tctgacgctg ggcaccggtg gtcgttatac tggctccagc
    12121 tatggtgatc cggctaactc ctttaaagtg ggaagttata cggtcgtgga tgcgttagtg
    12181 cgttatgatc tggcgcgagt cggcatggcg ggctccaacg tggcgctgca tgtcaacaac
    12241 ctgtttgatc gtgaatacgt cgccagctgc tttaacactt atggttgctt ctggggcgca
    12301 gaacgtcagg tcgttgcaac cgcaaccttc cgtttctaat ttctcttttg gggcacggat
    12361 ttccgtgccc gtttcacaag ttggctgtta tgcaggaata cacgaatcat tccgatacca
    12421 cttttgcact gcgtaatatc tcctttcgtg tgcccgggcg cacgcttttg catccgttgt
    12481 cgttaacctt tcctgccggg aaagtgactg gtctgattgg tcacaacggt tctggcaaat
    12541 ccactctgct caaaatgctt ggtcgtcacc agccaccgtc ggaaggggag attcttcttg
    12601 atgcccaacc gctggaaagc tggagcagca aagcgtttgc ccgcaaagtg gcttatttac
    12661 cgcagcagct tcctccggca gaagggatga ccgtgcgtga actggtggcg attggtcgtt
    12721 acccgtggca tggcgcgctg gggcgctttg gtgcggcaga tcgcgaaaag gtcgaggaag
    12781 ctatctcgct ggttggctta aaaccgctgg cgcatcggct ggtcgatagc ctctctggcg
    12841 gcgaacgtca gcgggcgtgg atcgccatgc tggtggcgca ggatagccgc tgtctgttgc
    12901 tcgacgaacc gacttcggcg ctggatatcg cccaccaggt tgatgtactg gcgctggtgc
    12961 atcgtttaag tcaggagcga ggcctgacgg tcattgccgt gctgcacgat ataaatatgg
    13021 cggcgcgcta ctgtgattat ctggtcgccc tgcgcggcgg tgaaatgatt gctcagggaa
    13081 cgcctgcgga aattatgcgc ggcgaaaccc tcgaaatgat ttatggcatc ccgatgggta
    13141 ttctgccgca tccggcgggt gctgcacctg tgagttttgt ttattgatga gcggcttacc
    13201 tcttatttcg cgccgccgac tgttaacggc gatggcgctt tctccgttgt tatggcagat
    13261 gaataccgcc cacgcggcag ttattgatcc caatcgtatt gtggcactgg agtggttgcc
    13321 ggtggagtta ctgctggcgc tcggcatcgt gccttacggc gtggcggaca ccatcaacta
    13381 tcgcctgtgg gtcagcgaac cgccattgcc ggactcagtg attgacgttg gtttgcgtac
    13441 agaacccaac cttgaactgc tgaccgaaat gaaaccgtcg tttatggtct ggtctgcagg
    13501 atatggccct tcaccagaaa tgctggcacg tattgcgccg gggcgcggat ttaacttcag
    13561 cgacggcaaa cagccactgg cgatggcgcg taaatcactg acggaaatgg cagatttact
    13621 taacctgcaa agcgcagcgg aaacgcattt agcgcactat gaagacttta tccgcagcat
    13681 gaaaccccgc tttgtgaagc gtggtgcacg cccgttattg ctgacgacgc ttatcgatcc
    13741 gcgccatatg ttggtcttcg gtccaaacag cttgttccag gaaattctcg atgagtacgg
    13801 catcccaaat gcctggcaag gggaaaccaa cttctggggc agtaccgccg tcagtatcga
    13861 tcgtctggca gcgtataaag acgttgatgt gctctgtttt gatcacgaca acagcaaaga
    13921 catggatgcg ctaatggcaa ccccgctgtg gcaggccatg ccgtttgtcc gcgccggacg
    13981 ctttcagcgc gtacctgcag tctggttcta tggtgcgacg ctttctgcaa tgcactttgt
    14041 gcgtattctg gataacgcca tcggaggtaa agcgtgagta aacgaattgc gctttttccg
    14101 gtgttattgc tggcgctgct ggtggttgct gctgcggcgt tgacctggat gaacttctcg
    14161 caggcgctgc cgcgcagcca gtgggcgcag gccgcctggt cgccggatat tgacgtcatc
    14221 gagcagatga tttttcacta cagcttgttg ccgcgtctgg cgatttcgct gctggtgggc
    14281 gcgggcctgg ggctggtggg cgtgctgttt cagcaagtgc tgcgtaaccc gctggcggaa
    14341 ccgacgacat tgggtgttgc aacaggcgcg caactgggga ttaccgtcac cacgctctgg
    14401 gcaatccccg gcgcgatggc gagccagttt gctgcgctgg caggggcttg tgttgttggc
    14461 ttaatcgtct ttggcgtcgc gtgggggaaa cgtctttcgc cggtaacgct gatcctcgcg
    14521 gggctggtag tgagccttta ttgcggtgca atcaatcagt tactggttat cttccatcat
    14581 gaccaactgc aaagcatgtt cctgtggagc accggaacgc tgacgcaaac cgactggggc
    14641 ggcgttgagc gtttatggcc gcagctgctg ggcggcgtga tgctgacgtt attgctactt
    14701 cgcccgttaa ctctgatggg gcttgatgat ggcgtggcgc gcaatctcgg gctggccttg
    14761 tcgctcgctc gtctggcggc gttgtcgctg gcgattgtca tcagtgcgct gctggtgaac
    14821 gcggtgggga ttatcggctt tatcgggttg ttcgcgccac tgctggcgaa aatgctgggg
    14881 gcgcggcgtt tgttgccacg gttgatgctg gcatcgctga ttggtgcgtt aattctgtgg
    14941 ctttctgatc aaatcatcct ctggctgact cgcgtgtgga tggaagtgtc caccggttcg
    15001 gtcactgcgt tgatcggtgc gccgctgcta ctgtggttgc tgccgcgttt acgcagcatt
    15061 agcgcgccgg atatgaaggt gaacgatcgt gtcgcgactg aacgccaaca cgtgctggcg
    15121 tttgccctcg cgggcggcgt gctgctgttg atggctgtgg tggtggcgct gtcgtttggt
    15181 cgtgatgcgc acggctggac gtgggcgagc ggggcgttgc tcgaggattt gatgccctgg
    15241 cgctggccgc gaattatggc tgcgctgttt gcgggcgtca tgctggcggt ggcgggctgt
    15301 attattcagc ggctgaccgg aaacccgatg gcaagcccgg aagtgctggg gattagctcc
    15361 ggcgcggcgt ttggcgtggt gttaatgctg tttctggtgc cgggtaatgc ctttggctgg
    15421 ctgttacccg caggcagtct cggggcggcg gtgacgctgt tgatcattat gatcgccgcc
    15481 ggacgtggtg gattttcccc acaccgcatg ttactggcgg gaatggcgtt aagtaccgcg
    15541 ttcaccatgc ttttgatgat gttgcaggca agtggtgacc cgcgaatggc gcaagttctg
    15601 acctggattt ccggttcgac ctacaacgcg accgatgcgc aggtctggcg taccggaatt
    15661 gtgatggtga ttttgctggc gattaccccg ctgtgccgcc gctggctgac cattttaccg
    15721 ttgggtggtg ataccgcacg agccgtgggg atggcgctga cgccgacgcg aattgcgctg
    15781 ctgctgttag cggcttgcct gacggcgacc gcgacaatga ccattggacc gttgagtttt
    15841 gttggtttaa tggcaccgca tattgcgcgg atgatgggct ttcgacggac gatgccacac
    15901 atcgtaattt cggcgctggt gggtggttta ctgctggtgt tcgctgactg gtgtgggcgg
    15961 atggtgctgt ttccattcca gatccccgcg gggctgctgt cgacctttat cggcgcgcca
    16021 tattttatct atttgttgag aaagcagagc cgttaattga atgcctcgcc cggtgggcga
    16081 ggcaaaatca tcacaacttc gcaaacaccc gacgtgcagc atcgatggtg ttattgatat
    16141 cttccatgct gtgcgccaca gacataaagc ccgcttcaaa cgctgacggt gccagataaa
    16201 caccttcgtc cagcatcata tggaagaaac gcttaaagcg ttccacgtca caggccatca
    16261 catcctgata gcacgtcacg gactcggcgt cggtaaagaa aataccgaac atgccgccaa
    16321 cgtggtttac caccagcgga attccggctt cttctgccgc ttcccgcaga ccttctgcca
    16381 gacgtgttgt cagctcatcc agcgtctcgt gaacgcccgg ctgtgcgact tcattcagac
    16441 aggcgaaacc agcagccatc gcaatcgggt taccggaaag cgtacccgcc tgatagaccg
    16501 gaccggtcgg ggccagcgca tccattacat cacgacgacc accgaatgcg cctaccggca
    16561 ttccaccgcc gatgattttg ccgaggcagg tgagatccgg ttccacaccg taataatcct
    16621 gtgcgccagc tagcgctacg cggaaaccgg tcatcacttc atcgatgatc agcaacgcgc
    16681 caaattcgtc gcacagcgcg cgcagacctg gcaggaactc tggcagcggt ggaacgcagt
    16741 tcatattgcc tgccaccggc tcgacgataa tacaggcaat ctcttgcggg tattgctcaa
    16801 acgcggcgcg tacagaggcc agatcgttat aagtacaggt taaggtatgt ttggcgaaat
    16861 ctgccggaac gcccggcgaa tttggctggc ctaacgtgag tgcgccagaa ccggctttca
    16921 ccagcaggca gtcagcgtga ccgtggtaac aaccttcaaa tttaataatt ttgtcgcgac
    16981 cggtaaaacc acgggccagg cggatggcgc tcatcgtcgc ttcagtgccg gagttcacca
    17041 tgcgcaccat atccatggtc gggaccagtt cggtcaccag ttgcgccatt ttcacttcca
    17101 tttcggttgg tgcaccaaag cttaaaccac gctcggcggc ttcaatcacg gcattgcgga
    17161 tcgccggatg gttatggccc agcaccatcg gcccccagga accgacataa tcgatatagg
    17221 ctttgccatc aacatcgtac agataagcgc cgtccgcttt ttcgataaac agtggagtgc
    17281 cgcccacgcc agtaaaggcg cgaacagggg agttcacacc gccagggatc agctcgcgcg
    17341 ctgcgctgta aagattttca gacttactca tggagggttc ctgattcgta gaaaaagtga
    17401 atggctgcta ttctatgtta ttcataacaa gttaaatact cgtcaaacat caggctgctt
    17461 gtactggtgc aatcctgatt tcttagagta taaaagtttt gtgcatttga aacattacgc
    17521 tttgcaaagg attttcatgg aacgtgcgag taaaatgccg tcatcttatt tgtatgacca
    17581 ataagtgatc attggatgaa aactgatact ccctctttag aaacaccgca ggccgcgcgc
    17641 ctgcgacgca gacaactgat tcgccaactt cttgagcgcg ataaaacccc gttagccatt
    17701 ttgtttatgg cggcagtcgt cggcacgctt gtcgggctgg ctgcggttgc ttttgacaaa
    17761 ggtgtcgcct ggttgcagaa ccaacgtatg ggggcactgg tacatactgc tgataattat
    17821 ccgcttctgt taaccgtcgc ttttctctgt tcggcggtgc tggcgatgtt cggctacttt
    17881 ttggtgcgca aatacgcgcc ggaagcaggt ggttcgggga tcccggaaat tgaaggggcg
    17941 ctggaagatc aacgtcccgt tcgctggtgg cgtgtgttgc cggtgaaatt ctttggcggg
    18001 ctgggaacac tcggcggagg catggtgttg gggcgcgaag ggccaaccgt gcagataggc
    18061 ggtaatatcg ggcggatggt actcgatgtt ttccgcctga aaggtgacga agctcgtcat
    18121 acgctactgg caaccggtgc tgctgcgggg ctggctgcgg cctttaacgc gccgctggcg
    18181 ggtattttgt ttattatcga agagatgcgt ccgcagtttc gctatacgtt aatttcgatt
    18241 aaagcggtat ttattggcgt cattatgtcg accattatgt accggatttt taatcatgaa
    18301 gttgcgttga ttgacgtcgg taaactttct gatgcgccgc ttaatacgct gtggctttat
    18361 ctgatcctcg gtattatttt tggcattttc ggccctattt ttaataaatg ggtgctgggg
    18421 atgcaggatt tgctgcaccg tgtgcacggt ggcaatatta ccaaatgggt actgatgggt
    18481 ggtgcgattg gcggtctgtg tggattgctg gggtttgtgg caccagcaac ttcgggcggc
    18541 ggttttaacc tgattcctat cgctaccgcg gggaatttca gcatgggaat gctggtgttt
    18601 atcttcgtcg cgcgggtcat taccacctta ctctgcttct cttccggcgc gccgggcggt
    18661 atttttgccc cgatgctggc gctgggtact gtgctgggaa ccgctttcgg aatggttgcc
    18721 gttgagttgt ttccgcaata tcaccttgag gcggggacgt ttgctattgc cggaatgggg
    18781 gcgttactgg cggcatctat tcgcgcgccg ttaacgggga tcattctggt tctggaaatg
    18841 accgataact accagctcat tttgccaatg attattaccg gtcttggcgc aacactatta
    18901 gcacaattta ccggtgggaa accgctatac tcggcgattc ttgcgcgcac gctggcaaaa
    18961 caggaagctg agcaactggc gcgaagcaag gccgcatcag cccgcgagaa tacttgaacg
    19021 aaataccagg gtattagata atggcgatta ttattgggtt agaatttgcc caattgccga
    19081 tgtcgtttgg agcaaaatat gagtgatgac gtagcactgc cgctggagtt taccgacgca
    19141 gcagccaaca aagttaaaag cctgatcgct gacgaagata acccgaatct gaaattacgc
    19201 gtgtatatca ccggtggcgg ttgcagcggc ttccagtatg gtttcacctt tgatgatcag
    19261 gtgaacgaag gcgatatgac catcgaaaaa cagggcgttg gcctggtggt tgatccgatg
    19321 agcctgcaat atctggtcgg cggttccgtt gactataccg aaggtctgga aggttctcgt
    19381 ttcatcgtga ccaacccgaa cgcgaaaagc acctgcggtt gcggttcttc cttcagcata
    19441 taatctgttg ttcatgtgcc ggataaacta tccggcacat cttctctctt caacgcccat
    19501 tctcatccag cgcaaacgtc ggtagcttaa gatgccaacg aatagccgcc agccgaatca
    19561 atagcgtcac gaccatgccc atcatactgg ctgtttccag tggtacggaa aatgtgtaat
    19621 aagccgtagc gtggacaata ccgccgataa tacaggcagt tgcgtagatt tctgtacgta
    19681 aaatcatggg gatttcgcgg gccagtacat cacgaatgat cccgccgcca acgccagtaa
    19741 tgacgcccat acaaaccgcg attaacggac cggcttccgc attaaaggct ttattcacgc
    19801 caatgccgac aaacaccgcc agaccaacgg cgtccagcac cggcaacatc cattttggta
    19861 agcgtcttgg ctggcgcacc agcacgatgg tcagcatgct ggtgaccatt gcaacgacca
    19921 gatcggtggg atctttcacc caaaataccg ggccgtgatc cagcgccatg tcgcgaattg
    19981 ttccgccgcc tactgcggta accacgccca ataccagaac gccaaaaggg tccatacgca
    20041 attttccggc taacaaaacg ccagagatag caaataccgc tgtgccgact atatccagcc
    20101 aatagacgag cattgttcaa tccccactga gcacctgaaa aggtcaggcg ctaatctacc
    20161 tgtgaaagcg cattacagag ctgttgtgca gcgaggataa tacgtgggct tgcacgttca
    20221 aaccagtcac tcgtgagagg aataacggga attttgagct gttcacccca gtattgtttg
    20281 attttaggaa tttggtccgg tccgcctgta atgacaatcg cctgtggcga gcgtgctaac
    20341 acctgttcgc ggctaacttg cggccaggga acccggctgt ctttaaagat gttttctccg
    20401 ccacaaactt cgagtacctg gttctgaatc gactcttttc cactggtaaa tggcggatta
    20461 atgccgaatt gcagaaaaac acgtttttta ggtttatcag catattgcgc tttcaattgc
    20521 gcgtactgat ccagcaggga ttgcgcggct tgttcggcct tatccggttg cggactccag
    20581 ggggccagtt gacgtaacgc attggcaatt tgttcaatgc ttgtcgcatc gacccacatc
    20641 acttttattc ccagcgaagc cagctggtca acctgccgct cggcattacc tccacgccag
    20701 gcaatcacca gatcgggttt cagcgcgaca atgcgttcca gattcatccc ctgccaggtg
    20761 gaaacctgct caatcttttg cgcttgtgga ggatagtcgg aatagctgct gaccccaacc
    20821 ggcgtgatcc cggcggcaaa ggcaagttca gtgttggcgg gagaaagcgt gatgacgcgc
    20881 ggcgcggcat tgagccacag cggcgcgaga aaagacaggg cgaccagcgc cctgaacagt
    20941 gacttagcca tgtgccagtt tctgcaccag tgactcaacc atcaggctgg actgtttagc
    21001 ggcaacagcc aggaactcat cgaagctaag atgagactgt tgatcggcca cgtcggagat
    21061 ggcgcgtact acgacaaacg ggacgttgaa attgtggcag acatgggcga ttgccgtcgc
    21121 ttccatctct acagcaatgg cctgtgggaa gttgtggcgg attttcgcca gaccaacaga
    21181 accgttgatg aaagcgtcgc cgctaacaat caggccacgt acagcgttaa gattcagttc
    21241 ggcaatgcag gcctcagcgg cagcgatcag tttatcgtca gctttgaagc ctgccgggca
    21301 gcctggtaac tgaccgtatt cataaccaaa tgccgtgaca tccgcgtcgt gataacgcgc
    21361 ttcgtccgag acaacgatat cgcccacttt cagcgttggt gccaggccac cggcagaacc
    21421 ggtgttaata atcacatctg gcttgcagtg ttccaacagc agagtggcac ccagcgccgc
    21481 agcgacttta ccgatgcccg atttcagaag cgcaacctcg gttccattca gttggccggt
    21541 atagattttg caaccgccca gactgatagt ttgacggttt tcgattttgt cacgcagcag
    21601 cgtaacttct tcttccattg caccaatgat gccgattttc atagatttac tcgcgataag
    21661 cccgatttga aggcatagtt taccatgcgc ttacggggaa gcgtatttct cacgcgggag
    21721 aggacatggc acagattgat ttccgaaaaa aaataaactg gcatcgtcgt taccgttcac
    21781 cgcagggcgt taaaaccgaa catgagattc tgcggatctt cgagagcgat cgcgggcgta
    21841 tcatcaactc tccggcaatt cgtcgtctgc aacaaaagac ccaggttttt ccactggagc
    21901 gcaatgccgc cgtgcgcacg cgtcttaccc actcgatgga agtccagcag gtggggcgct
    21961 acatcgccaa agaaatttta agccgtctga aagagcttaa attactggag gcgtatggtc
    22021 tcgatgaact gaccgggccc tttgaaagca ttgttgagat gtcatgcctg atgcacgata
    22081 tcggcaatcc gccgtttggt cattttggcg aagcggcgat aaatgactgg tttcgccagc
    22141 gtttgtaccc ggaagatgcc gaaagccagc ctctgactga cgatcgctgc agcgtggcgg
    22201 cactacgttt acgggacggg gaagaaccgc ttaacgcgct gcggcgcaag attcgtcagg
    22261 acttatgtca ttttgaaggt aacgcacagg gtattcgttt ggtgcataca ttgatgcgga
    22321 tgaatctcac ctgggcacag gttggcggta ttttaaaata tacccgtccg gcgtggtggc
    22381 gtggcgaaac gcctgagaca catcactatt taatgaaaaa gccgggttat tatctttctg
    22441 aagaagccta tattgcccgg ttgcgtaaag aacttaattt ggcgctttac agtcgttttc
    22501 cgttaacgtg gattatggaa gctgccgacg acatctccta ttgtgtggca gaccttgaag
    22561 atgcggtaga gaaaaggata tttaccgttg agcagcttta tcatcatttg cacgaggcgt
    22621 ggggccagca tgagaaaggt tcgctctttt cgctggtggt tgaaaatgcc tgggaaaaat
    22681 cacgctcaaa tagtttaagc cgcagtacgg aagatcagtt ttttatgtat ttacgggtaa
    22741 acaccctaaa taaactggta ccctacgcgg cacaacgatt tattgataat ctgcctgcga
    22801 ttttcgccgg aacgtttaat catgcattat tggaagatgc cagcgaatgc agcgatcttc
    22861 ttaagctata taaaaatgtc gctgtaaaac atgtgtttag ccatccagat gtcgagcagc
    22921 ttgaattgca gggctatcgg gtcattagcg gattattaga gatttatcgt cctttattaa
    22981 acctgccgtt atcagacttt actgaactgg tagaaaaaga acgggtgaaa cgtttcccta
    23041 ttgaaacgcg cttattccac aaactctcga cgcgccatcg gctggcctat gtcgaggctg
    23101 tcagtaaatt accgtcagat tctcctgagt ttccgctatg ggaatattat taccgttgcc
    23161 gcctgctgca ggattatatc agcggtatga ccgacctcta tgcgtgggat gaataccgac
    23221 gtctgatggc cgtagaacaa taaccaggct tttgtaaaga cgaacaataa atttttacct
    23281 tttgcagaaa ctttagttcg gaacttcgcg ctataaaacg aatctgaaga acacagcaat
    23341 tttgcgttat ctgttaatcg agactgaaat acatgaaaaa aaccacatta gcactgagtg
    23401 cactggctct gagtttaggt ttggcgttat ctccgctctc tgcaacggcg gctgagactt
    23461 cttcagcaac gacagcccag cagatgccaa gccttgcacc gatgctcgaa aaggtgatgc
    23521 cttcagtggt cagcattaac gtagaaggta gcacaaccgt taatacgccg cgtatgccgc
    23581 gtaatttcca gcagttcttc ggtgatgatt ctccgttctg tcaggagggt tctccgttcc
    23641 agagctctcc gttctgccag gggggcctgg gcggtaatgg cggcggccag caacagaaat
    23701 tcatggcgct gggttccggc gttatcattg atgccgataa aggctatgtc gtcaccaaca
    23761 accacgttgt tgataacgcg acggtgatta aagtccaact gagcgatggt cgtaagttcg
    23821 acgcgaagat ggttggcaaa gatccgcgct ctgatatcgc gctgatccag atccagaacc
    23881 cgaaaaacct gaccgcaatt aagatggcgg attctgatgc gctgcgcgtg ggtgattaca
    23941 ccgtagcaat tggtaacccg ttcggcctgg gcgagacagt aacttccggg attgtctctg
    24001 cgctgggacg tagcggcctg aacgctgaaa actacgaaaa cttcatccag actgatgcag
    24061 cgatcaatcg gggtaactca ggtggtgcgt tggttaacct gaacggcgaa ctgattggta
    24121 tcaacaccgc gattctcgca ccggacggcg gaaacatcgg tatcggtttt gctatcccga
    24181 gcaacatggt gaaaaacctg acctcgcaga tggtggaata cggccaggtg aaacgtggtg
    24241 agttgggtat tatgggcact gagctgaact ccgatctggc gaaagcgatg aaagttgacg
    24301 cccagcgcgg tgctttcgta agccaggttc tgccgaattc ttccgccgcg aaagcgggca
    24361 ttaaagcggg tgatgtgatc acctcactga acggtaagcc aatcagcagc tttgccgcac
    24421 tgcgtgctca ggtgggcact atgccggtag gtagcaaact gaccctgggc ttactgcgcg
    24481 acgggaagca ggttaacgtg aacctggaac ttcagcagag cagccagaat caggttgatt
    24541 ccagcaccat cttcaacggc attgaaggcg ctgagatgag caacaaaggt aaagatcagg
    24601 gcgtggtggt gaacaacgtg aaaacgggca ctccggctgc gcagatcggc ctgaagaaag
    24661 gtgatgtgat tattggcgcg aaccagcagg cagtgaaaaa catcgctgaa ctgcgtaaag
    24721 ttctcgacag caaaccgtct gtgctggcac tgaacattca gcgcggcgac agcaccatct
    24781 acctgttaat gcagtaatct ccctcaaccc cttcctgaat acgggaaggg gttctcctta
    24841 caatctgtga acttcgccac aactccatac atcttcatca tcctttgggc atttgcacaa
    24901 tgccgtacgt tacgtacttc cttatgctaa gccgtgcata acggaggact tatggctggc
    24961 tggcatcttg ataccaaaat ggcgcaggat atcgtggcac gtaccatgcg catcatcgat
    25021 accaatatca acgtaatgga tgcccgcggg cgaattatcg gcagcggcga tcgtgagcgt
    25081 attggtgaat tgcacgaagg agcattgctg gtactttcac aaggacgagt cgtcgatatc
    25141 gatgacgcgg tggcacgtca tctgcacggt gtgcggcagg ggattaatct accgttacgg
    25201 ctggaaggtg aaattgtcgg cgtaattggc ctgacaggtg aaccagagaa tctgcgtaaa
    25261 tatggcgaac tggtctgcat gacggctgaa atgatgctgg aacagtcgcg gttgatgcac
    25321 ctgttggcgc aggatagccg tttgcgggaa gaactggtga tgaacctcat tcaggctgag
    25381 gagaatactc ccgcacttac tgaatgggcg caacggctgg ggatcgatct caatcaaccg
    25441 cgagtggtgg ctattgttga ggtcgacagc ggtcagcttg gcgtggacag cgcaatggcg
    25501 gagttacagc aactgcaaaa tgcgctgact acgccggagc gtaataatct ggtggcgatt
    25561 gtctcgctaa ccgaaatggt ggtgttgaaa ccggcgttga actcttttgg gcgctgggac
    25621 gcagaagatc atcgtaagcg agttgaacaa ctgattaccc gcatgaaaga gtatggccag
    25681 ctgcgttttc gcgtttcgct gggcaactat tttaccggtc ctggcagtat tgcccgatcc
    25741 tatcgtacgg cgaaaacgac gatggtggtg ggtaaacagc ggatgccaga aagtcgctgc
    25801 tatttttatc aggatctgat gttacctgtg ttactcgaca gtttgcgtgg cgactggcag
    25861 gccaacgaac tggcgcgacc gctggcgcgg ctgaaagcga tggacaataa cggcttgctg
    25921 cggcgaacgc tggcggcgtg gtttcgccac aatgtgcaac cgctggcaac gtcaaaggcg
    25981 ttgtttattc atcgtaatac tctggaatat cggcttaatc gtatatcgga actgaccggg
    26041 cttgatttgg gcaattttga tgacaggttg ctgctgtatg tggcgttaca actggatgaa
    26101 gagcggtagg tcgattacaa aattacagcc gggtgagact ttacccggcc acagaatggc
    26161 attatttacg cgtcagtttt tccagatcgg cttcaatctc gctgatctta ttggttacta
    26221 ctgactccag gtgacgtaag tcgtcgagga tcttacgctt aagatcaact tcgctgcggt
    26281 cacgctggca gatttgatca agctcatcaa tgatataccg tagattcggg ctaatttcct
    26341 ggacttcttt ataaccctga cccacaccat cagcgacgac cgttttacgc tgacgcgggt
    26401 atttaaactt cacgctcttg gcgaaaaact cgcctttgtc tttctggaaa tagattttca
    26461 gaatatcgtt gttggcttcc tgccggaggc tgtaacgatc aatttcttca ggattggtaa
    26521 tacccagact tttcagattg tcgtacatag cgttacctca aaatgagtca gtaaattgtg
    26581 cttattttag catttggcct ggcccgcccg gctcgactgt gatcgcagaa agcctttttc
    26641 ttccagactt ctatcatggc gtaaaaatca aaaaattacc tgctttattg tggtgatatt
    26701 ccatggaatg gattagcaca tttatcttca ttttgcgaat tacatgacag tccgaaacaa
    26761 gatgaaaaag tatttaaaaa agggggaaaa tgagtaggac gaattattct gagccagaaa
    26821 gcaatcgggc agcgtcgtgc tgcccgtgta catactttta gtcgatggta cgcagcagtt
    26881 cgttaatacc aactttgccg cgagttttcg cgtcaacttt cttaacgata actgcacagt
    26941 agaggctgta tttgccatct ttcgacggca gattacctga aacaaccaca gaccctgccg
    27001 gaacgcgacc gtagtggatt tcgccggttt cacggtcata aatacgggtg ctctgaccaa
    27061 tgtatacgcc catagagatg acggaacctt cttcgacaat caccccttca accacttcag
    27121 agcgtgcgcc gatgaagcag ttatcttcaa taatggtcgg gttagcctgc agcggctcca
    27181 gcacgccgcc gatacccacg ccaccggaaa ggtggacgtt tttaccaatc tgcgcacagg
    27241 aaccgacggt cgcccaggta tcaaccatgg tgccttcatc aacatatgcg ccgatgttga
    27301 cgtaagacgg catcagcacg gtgttacggg caatgaacgc gccctgacgc actgccgctg
    27361 gtggcacgac gcggaagcct tctttctgga aacgtgcttc atcgtagtta gcgaatttca
    27421 tcggcacttt gtcgaagtag cggctttctg ccccttcgat cacctgatta tcattaatac
    27481 ggaaagagag cagcaccgct tttttcaacc actgatgcgt cacccactga ccgtcaattt
    27541 tttccgctac acgcagtgcg ccggaatcca gcagggcgat cacctgattt accgcttcgc
    27601 gggtaacggt gtctgcattg gctggcgtga tctcggcacg gcgttcaaaa gcggtttcaa
    27661 taatgttctg taactgctgc attgttaaac tcttttcata tcagtaaaca catcaccctt
    27721 tatcgtttgg attgagggcc tctgtcaacc gctgatgcac ttcctgctgc aactcgttat
    27781 taagcgcacg ccggtcagcg gtggcaatta tgaataaatc ttctactcgc tcgccaatgg
    27841 ttgtaattcg ggcgccatga agcgaaattc ccagatcggc aaaaattttc ccgactcgcg
    27901 ccagcagtcc aggttggtcg agggcgatca gttcgaggaa cgatttgcgg tcggtatggg
    27961 tcggcaaaaa cgtcacttcg gtttcaacag taaaatggcg caatttagcg ggttggcgac
    28021 ggggctgcgg tggctgccag ctactttgtg tcagtacctg ctccagacca aatcgaataa
    28081 cttcatggcg atctgcggaa agtgggctgc cgtccggctc aaggacaatg aatgtatcca
    28141 tcgccatatc gtcgcgggtg gtaaaaattt gtgcgtcgtg aacgcttaag ttgcgtcgat
    28201 ctaactcggc acaaacagcg gcaaacaagt aaggacggtc cgggctccag ataaaaatct
    28261 cggtgccacc acgcgtggcg tgtgggctta gcaacactaa tgacttactt aaatcatgct
    28321 gtagcaaatg gcgtgcgtgc caggccagtt gatttggact gtgacgaaca aaatagtttg
    28381 cccggcagcg tgaccagata tggtgcagtg cctcttcgtc aatattgtcc atacgcagca
    28441 gcgccagcgc ctggagctgg tgatggcgaa cccgttcgcg catatccggc gtgttttgca
    28501 ttccgcgccg tagctgcttt tcggtagcaa agtagagctc acgcaacaga ctttgcttcc
    28561 agctattcca cagtgtttcg ttggtggcgc atatatcggc cactgtcagg cataccagat
    28621 aacgaagtcg gttttctgtt tgcacttctt cggcaaactg cttgatcact tccgggtcct
    28681 gaatatcgcg gcgttgggcg gtcaccgaca tcaacaggtg ctggcgaacc agccacgcca
    28741 ccagttgcgt ttcgcgcgag ttaagcccgt ggagttcggc aaaatgcacc acgtcttgtg
    28801 caccgagaat ggagtggtcg ccgccacgcc ctttggcgat atcgtgaaac agcgcagcga
    28861 tgaaaatcag ctcaggtgac ggcagtcgtg gccagagatc aacacataat ggatgacgtt
    28921 ggcgcgtttc ttcactggcg aaactctcca gtttcagcat cacgcgaata gtatgttcat
    28981 ccaccgtgta ggcgtggaac aggtcaaact gcatctgccc gacgatatgc gaccattggg
    29041 gcatatacgc gccgagcacg ctatgacgat gcatcggtaa tagtccgcga cgcaccgcac
    29101 cagggtgacg cagaatgctc aaaaacagtt ttcgcgcttg cggaatatta cacaacggtt
    29161 gttgcagatg gcgacgggca tggcgcagat ggcgcagcgt ggtggagtaa atgccggtga
    29221 tcgcactgtt gcgcaccatg gtgtagaaca tacgcaagat ggcttccggc tggcgcataa
    29281 atagtgtttc atcacgcagg tcgattagcg taccgcgtag ctgaaactca tcgtcgattg
    29341 gacgtggttt ttcgtcggcg ggaagggcga ggatggcttc atcaaacagt tgcagcagca
    29401 tctggttgag ttcactgacg cggcgtgtaa cgcggaagta atccttcatc atccgctcga
    29461 ccggttcgtt accttcacca ctgtaattca aacgctgggc gacgctaagc tggcgatcga
    29521 ataacaggcg attatcgtaa cggctgacga ccagatgcag ggcaaagcga atgcgccaca
    29581 atatatgcag acattcgttt aattctgccc gctccgctga ggttaagaag ccaaacccga
    29641 ccatttcatc cagcgatgtt gcgccaaaat gacggcgggc aacccattgc agagtgtgaa
    29701 tatcgcgcaa gccgccaggg ctacttttga tgtctggttc aaggttgtag ctggtgccgt
    29761 ggtaacgctg atggcgctgg ttctgttctt caactttcgc tgcgtagaac ttgtcggaag
    29821 gccagaatcc ttcgctaaaa atatgttttt gcagttcgag gaacagcgct acatcgccaa
    29881 ttaataagcg ggattcgatt aaattggtgg cgacggttaa atccgataac ccttccagca
    29941 tgcactcttc aagcgtgcgc acgctatgac cgacttccag tttcacatcc cagagcagcg
    30001 ttaacagctc gcccactttt tgcgcctgat catccgggag ctttttacgg cttaaaatca
    30061 gtaaatcgat gtcggaaagt ggatgcagct cgccacgacc ataaccaccg acggcaacca
    30121 atgccaggtc ggcaatctgg ctgaatcccg cttcaatcca taatcgttgc aggagctggt
    30181 ctatgaactc ggtacgtgcc tcaatcaact gttctgcgga gatcccattg tcaaaggcat
    30241 cacccagcca acgctggaaa gtatcgatat gggcttttat cccaccgacg gttaattcat
    30301 cacggggcca ggcgcatgga ttttgcggtt gaccgggcag ggtggggaga gcggtgtttg
    30361 cgtactgttc tggaagggta ttcattgtgc gccacccata agattaaatt attgcattaa
    30421 aaaagccggc attcgccggc ttcattttat tcgtcgtgcg agattatcgc cgggatggtg
    30481 tcatccttgc gtagcgtcag aatttcgcag ccgttatcag tcaccacaat agtatgctca
    30541 tattgtgcag acaagctgcg atctttggtt tttaccgtcc agccatcttt catggtgcgg
    30601 atctcttttt tacccgcgtt gaccattggc tcgatggtga acgtcatccc cggtttcagc
    30661 acgacgttgg tttcacggga gtcatagtgc agcacctgcg gttcttcgtg gaagccgcga
    30721 ccaataccgt gtccacaata ttcacgaacg acggagaagc cttctgcttc gacaaatttc
    30781 tgaattgccg caccgatttc gcgcagatta atgccaggtt ttaccatgcg tagcgccagg
    30841 tacaggcttt cttgcgtgat gcggcacaga cgttcgccca tgatggtcgg cttaccgacg
    30901 ataaacattt tcgaggtatc gccgtggaaa ccatctttga ttacggtgac atcaatgtta
    30961 acgatatcgc catctttcag cagcttggcg tcgtccggga taccgtggca caccacttca
    31021 ttaatagaga tgcaaacgga tttcggatag ccgtgatagc cgaggcaggc agaaaccgcg
    31081 tgttgttcat taacaatgta atcattacag atgcgatcca gctcgccggt gctgacgccc
    31141 ggtttaacat acggttcgat catctccagc acttcggcag ccagtcggcc agcgacgcgc
    31201 attttttcga tatcttctgg ggtcttgatt gagatagcca ttaattctgt ccatcagcgt
    31261 cggtgatacc gacaatatat atgtaagtgc cgtcaatggt atcacacccg ggcaaattga
    31321 gaatcattct gaatttcgcc aaacgtgcca ctgaaggttt tctataatag aaaattcaac
    31381 gtctgatgct gtacacagcg ccaacaatta ttggtgtcca cgacgtattt gtggtataaa
    31441 gcgcgccgga cttccgatcc atttcgtata cacagactgg acggaagcga caatctcact
    31501 ttgtgtaaca acacacacgt atcggcacat attccggggt gccctttggg gtcggtaata
    31561 tgggatacgt ggaggcataa ccccaacttt tatatagagg ttttaatcat ggcaactgtt
    31621 tccatgcgcg acatgctcaa ggctggtgtt cacttcggtc accagacccg ttactggaac
    31681 ccgaaaatga agccgttcat cttcggtgcg cgtaacaaag ttcacatcat caaccttgag
    31741 aaaactgtac cgatgttcaa cgaagctctg gctgaactga acaagattgc ttctcgcaaa
    31801 ggtaaaatcc ttttcgttgg tactaaacgc gctgcaagcg aagcggtgaa agacgctgct
    31861 ctgagctgcg accagttctt cgtgaaccat cgctggctgg gcggtatgct gactaactgg
    31921 aaaaccgttc gtcagtccat caaacgtctg aaagacctgg aaactcagtc tcaggacggt
    31981 actttcgaca agctgaccaa gaaagaagcg ctgatgcgca ctcgtgagct ggagaaactg
    32041 gaaaacagcc tgggcggtat caaagacatg ggcggtctgc cggacgctct gtttgtaatc
    32101 gatgctgacc acgaacacat tgctatcaaa gaagcaaaca acctgggtat tccagtattt
    32161 gctatcgttg ataccaactc tgatccggac ggtgttgact tcgttatccc gggtaacgac
    32221 gacgcaatcc gtgctgtgac cctgtacctg ggcgctgttg ctgcaaccgt acgtgaaggc
    32281 cgttctcagg atctggcttc ccaggcggaa gaaagcttcg tagaagctga gtaataaggc
    32341 acgctcgtac gagcccttat agaccaggta gtacacgttt ggttaggggg cctgcatatg
    32401 gccccctttt tcacttttat atctgtgcgg tttaatgccg ggcagatcac atctccgagg
    32461 attttagaat ggctgaaatt accgcatccc tggtaaaaga gctgcgtgag cgtactggcg
    32521 caggcatgat ggattgcaaa aaagcactga ctgaagctaa cggcgacatc gagctggcaa
    32581 tcgaaaacat gcgtaagtcc ggtgctatta aagcagcgaa aaaagcaggc aacgttgctg
    32641 ctgatggcgt gatcaaaacc aaaatcgacg gcaactacgg catcattctg gaagttaact
    32701 gccagactga cttcgttgca aaagacgctg gtttccaggc gttcgcagac aaagttctgg
    32761 acgcagctgt tgctggcaaa atcactgacg ttgaagtact gaaagcacag ttcgaagaag
    32821 aacgtgttgc gctggtagcg aaaattggtg aaaacatcaa cattcgccgc gttgctgcgc
    32881 tggaaggcga cgttctgggt tcttatcagc acggtgcgcg tatcggtgtt ctggttgctg
    32941 ctaaaggcgc tgacgaagag ctggttaaac acatcgctat gcacgttgct gcaagcaagc
    33001 cagaattcat caaaccagaa gacgtatccg ctgaagtggt agaaaaagaa taccaggtac
    33061 agctggatat cgcgatgcag tctggtaagc cgaaagaaat cgcagagaaa atggttgaag
    33121 gccgcatgaa gaaattcacc ggcgaagttt ctctgaccgg tcagccgttc gttatggaac
    33181 caagcaaaac tgttggtcag ctgctgaaag agcataacgc tgaagtgact ggcttcatcc
    33241 gcttcgaagt gggtgaaggc atcgagaaag ttgagactga ctttgcagca gaagttgctg
    33301 cgatgtccaa gcagtcttaa ttatcgaaaa ggagccgcct gagggcggct tctttttgtg
    33361 cccatcttgt aaattcagct aacccttgtg gggctgcgct gaaaagcgac gtacaatgtc
    33421 gctagtatta attcatttca atcgttgaca gtctcaggaa agaaacatgg ctaccaatgc
    33481 aaaacccgtc tataaacgca ttctgcttaa gttgagtggc gaagctctgc agggcactga
    33541 aggcttcggt attgatgcaa gcatactgga tcgcatggct caggaaatca aagaactggt
    33601 tgaactgggt attcaggttg gtgtggtgat tggtgggggt aacctgttcc gtggcgctgg
    33661 tctggcgaaa gcgggtatga accgcgttgt gggcgaccac atggggatgc tggcgaccgt
    33721 aatgaacggc ctggcaatgc gtgatgcact gcaccgcgcc tatgtgaacg ctcgtctgat
    33781 gtccgctatt ccattgaatg gcgtgtgcga cagctacagc tgggcagaag ctatcagcct
    33841 gttgcgcaac aaccgtgtgg tgatcctctc cgccggtaca ggtaacccgt tctttaccac
    33901 cgactcagca gcttgcctgc gtggtatcga aattgaagcc gatgtggtgc tgaaagcaac
    33961 caaagttgac ggcgtgttta ctgctgatcc ggcgaaagat ccaaccgcaa ccatgtacga
    34021 gcaactgact tacagcgaag tgctggaaaa agagctgaaa gtcatggacc tggcggcctt
    34081 cacgcttgct cgtgaccata aattaccgat tcgtgttttc aacatgaaca aaccgggtgc
    34141 gctgcgccgt gtggtaatgg gtgaaaaaga agggacttta atcacggaat aattcccgtg
    34201 atggataaat aagggtaaga ttccgcgtaa gtatcgcggg ggatcttacc cgtaatatgt
    34261 ttaatcaggg ctatacttag cacacttcca ctgtgtgtga ctgtctggtc tgactgagac
    34321 aagttttcaa ggattcgtaa cgtgattagc gatatcagaa aagatgctga agtacgcatg
    34381 gacaaatgcg tagaagcgtt caaaacccaa atcagcaaaa tacgcacggg tcgtgcttct
    34441 cccagcctgc tggatggcat tgtcgtggaa tattacggca cgccgacgcc gctgcgtcag
    34501 ctggcaagcg taacggtaga agattcccgt actctgaaaa tcaacgtgtt tgatcgttca
    34561 atgtctccgg ccgttgaaaa agcgattatg gcgtccgatc ttggcctgaa cccgaactct
    34621 gcgggtagcg acatccgtgt tccgctgccg ccgctgacgg aagaacgtcg taaagatctg
    34681 accaaaatcg ttcgtggtga agcagaacaa gcgcgtgttg cagtacgtaa tgtgcgtcgt
    34741 gacgcgaacg acaaagtgaa agcactgttg aaagataaag agatcagcga agacgacgat
    34801 cgccgttctc aggacgatgt acagaaactg actgatgctg caatcaagaa aattgaagcg
    34861 gcgctggcag acaaagaagc agaactgatg cagttctgat ttcttgaacg acaaaaacgc
    34921 cgctcagtag atccttgcgg atcggctggc ggcgttttgc tttttattct gtctcaactc
    34981 tggatgtttc atgaagcaac tcaccattct gggctcgacc ggctcgattg gttgcagcac
    35041 gctggacgtg gtgcgccata atcccgaaca cttccgcgta gttgcgctgg tggcaggcaa
    35101 aaatgtcact cgcatggtag aacagtgcct ggaattctct ccccgctatg ccgtaatgga
    35161 cgatgaagcg agtgcgaaac ttcttaaaat gatgctacag caacagggga gccgcaccga
    35221 agtcttaagt gggcaacaag ccgcttgcga tatggcagcg cttgaggatg ttgatcaggt
    35281 gatggcagcc attgttggcg ctgctgggct gttacctacg cttgctgcga tccgcgcggg
    35341 taaaaccatt ttgctggcca ataaagaatc actggttacc tgcggacgtc tgtttatgga
    35401 cgccgtaaag cagagcaaag cgcaattgtt accggtcgat agcgaacata acgccatttt
    35461 tcagagttta ccgcaaccta tccagcataa tctgggatac gctgaccttg agcaaaatgg
    35521 cgtggtgtcc attttactta ccgggtctgg tggccctttc cgtgagacgc cattgcgcga
    35581 tttggcaaca atgacgcccg atcaagcctg ccgtcatccg aactggtcga tgggacgtaa
    35641 aatttctgtc gattcggcta ccatgatgaa caaaggtctg gaatacattg aagcgcgttg
    35701 gctgtttaac gccagcgcca gccagatgga agtgctgatt cacccgcagt cagtgattca
    35761 ctcaatggtg cgctatcagg acggcagtgt tctggcgcag ctgggggaac cggatatgcg
    35821 tacgccaatt gcccacacca tggcatggcc gaatcgcgtg aactctggcg tgaagccgct
    35881 cgatttttgc aaactaagtg cgttgacatt tgccgcaccg gattatgatc gttatccatg
    35941 cctgaaactg gcgatggagg cgttcgaaca aggccaggca gcgacgacag cattgaatgc
    36001 cgcaaacgaa atcaccgtag ctgcttttct tgcgcaacaa atccgcttta cggatatcgc
    36061 cgcgttgaat ttatccgtac tggaaaaaat ggatatgcgc gaaccacaat gtgtggacga
    36121 tgtgttatct gttgatgcga acgcgcgtga agtcgccaga aaagaggtga tgcgtctcgc
    36181 aagctgagga taatccggct acagagagtc gcgctatttg ttagcgtagg gcttcagtga
    36241 tatagtctgc gccatctgat cgtaagtagt tggctttata aggtcaggta tgccgtggtt
    36301 ttacacggct tttttttgta taggcttcag tattcctgag taccgtaaac cctgtcaggg
    36361 aataagaaac gcgtgatgtt gtctgctact caaccactta gcgaaaaatt gccagcgcat
    36421 ggctgtcgtc atgttgcgat cattatggac ggcaatggtc gctgggcaaa aaagcaaggg
    36481 aagattcgtg cctttgggca taaagccggg gcaaaatccg tccgccgggc tgtctctttt
    36541 gcggccaaca acggtattga ggcgttaacg ctgtatgcct ttagtagtga aaactggaac
    36601 cgaccagcgc aggaagtcag tgcgttaatg gaactgtttg tgtgggcgct agatagcgaa
    36661 gtaaaaagtc tgcaccgaca taacgtgcgt ctgcgtatta ttggcgatac cagtcgcttt
    36721 aactcgcgtt tgcaagaacg tattcgtaaa tctgaagcgc taacagccgg gaataccggt
    36781 ctgacgctga atattgcggc gaactacggt ggacgttggg atatagtcca gggagtcagg
    36841 caactggctg aaaaggtgca gcaaggaaac ctgcaaccag atcagataga tgaagagatg
    36901 ctaaaccagc atgtctgtat gcatgaactg gcccctgtag atttagtaat taggactggg
    36961 ggggagcatc gcattagtaa ctttttgctt tggcaaattg cctatgccga actttacttt
    37021 acagatgttc tctggcccga tttcgatgaa caagactttg aaggggcgtt aaatgccttt
    37081 gctaatcgag agcgtcgttt cggcggcacc gagcccggtg atgaaacagc ctgatggggg
    37141 tcgcttttgc tgaagtatcg cctgatatct gcttttgtgt taatacccgt cgtcatcgcg
    37201 gcgttgtttc tgttgccgcc ggtggggttc gccattgtaa cgctggtggt ctgtatgctg
    37261 gcagcgtggg aatggggaca gcttagcggt tttaccactc gttcgcagcg agtatggttg
    37321 gcggtgttat gcgggttatt gttggcgctg atgctttttc tgttgccgga atatcaccga
    37381 aatattcatc aaccgctggt tgaaatctca ctttgggctt cgctgggttg gtggattgtc
    37441 gcgctattgc tggtgctgtt ttacccaggt tccgcagcaa tctggcgtaa ctctaaaaca
    37501 ttgcgcatta tttttggcgt gctaaccatt gttcccttct tctggggcat gctggcgtta
    37561 cgggcctggc actacgacga gaatcattac agtggcgcaa tatggctgct ctatgtcatg
    37621 atcctggtat ggggcgctga ctctggcgca tatatgtttg gcaaattgtt tggtaaacat
    37681 aaactggcac cgaaggtttc tccgggtaaa acctggcaag gctttatcgg tggactcgcg
    37741 accgcagcgg taatctcatg gggttatggc atgtgggtga atctcgacgt cgctcccgtc
    37801 accttactca tttgctctat tgtcgcagcg ttagcctcag tgctcggcga tctgaccgaa
    37861 agtatgttta agcgtgaagc aggaattaag gacagcggtc atttaattcc aggacacggt
    37921 ggtattttag atcgtattga tagcctgacg gctgcggtac cggtctttgc ttgcttgttg
    37981 ttactggtat tcaggacgct ttaacggaag gtaatatgct gagttttctc tgggatttgg
    38041 cttcgttcat cgttgcactg ggtgtactta tcaccgtgca tgaatttggt catttctggg
    38101 ttgcccggcg ttgtggtgtt cgcgttgagc gtttctcaat agggtttggt aaggcgctct
    38161 ggcggcgaac tgataagctc ggcaccgaat atgttatcgc cctgatcccg ttgggcggtt
    38221 atgtcaaaat gctggatgag cgcgcagaac cggtcgttcc ggaactccgc caccatgcct
    38281 tcaataataa atctgtcggc caacgagcgg cgattattgc cgcaggtccg gttgcaaact
    38341 tcatttttgc tatctttgcc tactggctgg tttttattat tggtgtgcct ggcgtacgtc
    38401 cggtggttgg tgaaatagca gccaattcga tagctgcgga agcacaaatt gcaccaggta
    38461 cggaactaaa agccgtagat ggtatcgaaa cgcctgattg ggatgccgtg cgtttgcagt
    38521 tggtcgataa aattggcgat gaaagcacca ccattacggt agcgccattt ggcagcgacc
    38581 aacggcggga tgtaaagctc gatttacgtc actgggcgtt tgagcctgat aaagaagatc
    38641 cggtatcttc gttggggatt cgtcctcgtg ggccgcaaat tgaacctgta ctggaaaatg
    38701 tgcagccaaa ctcggcggca agcaaggcag gtttgcaagc aggcgacagg atcgttaaag
    38761 tcgatggtca gcccttaacg cagtgggtga cctttgtgat gcttgtccgg gataacccgg
    38821 gtaaatcctt agcgttagaa atcgaaaggc aggggagtcc cttgtctttg acattaatcc
    38881 cggagagtaa accgggtaag ggtaaagcga ttggttttgt cggtattgag ccgaaagtca
    38941 ttcctttgcc agatgagtat aaagttgtac gccagtatgg gccgttcaac gctatcgtcg
    39001 aagccacgga caaaacgtgg cagttgatga agctaacggt cagtatgctg ggaaaattga
    39061 tcaccggtga tgtgaaactg aacaacctca gtgggccgat ctctatcgcc aagggggctg
    39121 ggatgacagc ggaactcggg gtagtttatt acctgccgtt tcttgcgctt attagcgtga
    39181 acttagggat aattaacctg tttccgttgc ccgtacttga cggggggcat ctgctgttcc
    39241 ttgcgatcga aaagatcaag ggcggaccgg tatccgagcg ggttcaagac ttttgttatc
    39301 gcattggctc gattctgctg gtgctgttaa tggggcttgc acttttcaat gatttctctc
    39361 ggttatgaga gttagttagg aagaacgcat aataacgatg gcgatgaaaa agttgctcat
    39421 agcgtcgctg ctgtttagca gcgccaccgt atacggtgct gaagggttcg tagtgaaaga
    39481 tattcatttc gaaggccttc agcgtgtcgc cgttggtgcg gccctcctca gtatgccggt
    39541 gcgcacaggc gacacggtta atgatgaaga tatcagtaat accattcgcg ctctgtttgc
    39601 taccggcaac tttgaggatg ttcgcgtcct tcgtgatggt gatacccttc tggttcaggt
    39661 aaaagaacgt ccgaccattg ccagcattac tttctccggt aacaaatcgg tgaaagatga
    39721 catgctgaag caaaacctcg aggcttctgg tgtgcgtgtg ggcgaatccc tcgatcgcac
    39781 caccattgcc gatatcgaga aaggtctgga agacttctac tacagcgtcg gtaaatatag
    39841 cgccagcgtt aaagctgtcg tgaccccgct gccgcgcaac cgtgttgacc taaaactggt
    39901 gttccaggaa ggtgtgtcag ctgaaatcca gcaaattaac attgttggta accatgcttt
    39961 caccaccgat gaactgatct ctcatttcca actgcgtgac gaagtgccgt ggtggaacgt
    40021 ggtaggcgat cgtaaatacc agaaacagaa actggcaggc gaccttgaaa ccctgcgcag
    40081 ttactatctg gatcgcggtt atgcccgttt caacatcgat tctacccagg tcagtctgac
    40141 gccagataaa aaaggtattt acgtcacggt gaacatcacc gaaggcgatc agtacaagct
    40201 ttctggcgtt gaagttagcg gcaatcttgc cgggcactcc gctgaaattg agcaactgac
    40261 taagatcgag ccgggcgagc tgtataacgg caccaaagtg accaaaatgg aagatgacat
    40321 taaaaagctt cttggtcgct atggttatgc ctatccgcgc gtacagtcga tgcctgaaat
    40381 taacgatgcc gacaaaaccg ttaaattacg cgtgaacgtt gatgcgggta accgtttcta
    40441 tgtgcgtaag atccgttttg aaggtaacga tacctcgaaa gatgccgtcc tgcgtcgcga
    40501 aatgcgtcag atggaaggtg catggctggg gagcgatctg gtcgatcagg gtaaggagcg
    40561 tctgaaccgt ctgggcttct ttgaaactgt cgataccgat acccaacgtg ttccggggag
    40621 cccggatcag gttgatgtag tttacaaggt gaaagaacgt aacaccggta gcttcaactt
    40681 cgggattggt tacggtactg aaagtggcgt gagcttccag gctggtgtgc aacaggataa
    40741 ctggttaggt acgggttatg ctgttggtat caacgggacc aaaaacgatt accagaccta
    40801 tgctgaactg tcggtaacca acccgtactt caccgtagat ggtgtaagcc tcggtggtcg
    40861 tctcttctat aatgacttcc aggcagatga cgccgacctg tccgactata ccaacaagag
    40921 ttatggtaca gacgtgacgt tgggcttccc gatcaacgaa tataactcgc tgcgtgcagg
    40981 tctgggttat gtacataact ccctgtccaa catgcagcct caggttgcga tgtggcgtta
    41041 tctgtactct atgggtgaac atccgagcac ctctgatcag gataacagct tcaaaacgga
    41101 cgacttcacg ttcaactatg gctggaccta taacaagctt gaccgtggtt acttcccgac
    41161 agatggttca cgtgtcaacc tgaccggtaa agtgaccatt ccgggatcgg ataacgaata
    41221 ctacaaagtg acgttagaca cggcgactta tgtgccgatt gatgacgatc acaaatgggt
    41281 tgttctgggt cgtacccgct ggggttatgg tgatggttta ggcggcaaag agatgccgtt
    41341 ctatgagaac ttctatgccg gtggttccag caccgtgcgt ggcttccagt ccaataccat
    41401 tggtccgaaa gcagtttact tcccgcatca ggccagtaat tatgatccgg actatgatta
    41461 cgaatgtgcg actcaggacg gcgcgaaaga tctgtgtaaa tcggatgatg ctgtaggcgg
    41521 taacgccatg gcggttgcca gcctcgagtt catcaccccg acgccgttta ttagcgataa
    41581 gtatgctaac tcggttcgta cttccttctt ctgggatatg ggtaccgttt gggatacaaa
    41641 ctgggattcc agccaatatt ctggatatcc ggactatagt gatccaagca atatccgcat
    41701 gtctgcgggt atcgcattac aatggatgtc cccattgggg ccgttggtgt tctcctacgc
    41761 ccagccgttc aaaaagtacg atggagacaa agcagaacag ttccagttta acatcggtaa
    41821 aacctggtaa gtgttctcca caaaggaatg tagtggtagt gtagcgatga cttttggcga
    41881 tcaatataag atcgccgggc cacgcaaaga actgcaccct ccggtgcaaa tgggatggta
    41941 aggagtttat tgtgaaaaag tggttattag ctgcaggtct cggtttagct ctggcaactt
    42001 ctgctcaggc ggctgacaaa attgcaatcg tcaatatggg cagcctgttc cagcaggtag
    42061 cgcagaaaac cggtgtttct aacacgctgg aaaacgagtt caaaggccgt gccagcgaac
    42121 tgcagcgtat ggaaaccgat ctgcaggcta aaatgaaaaa gctgcagtcc atgaaagcgg
    42181 gcagcgatcg cactaagctg gaaaaagacg tgatggctca gcgccagact tttgctcaga
    42241 aagcgcaggc ttttgagcag gatcgcgcac gtcgttccaa cgaagaacgc ggcaaactgg
    42301 ttactcgtat ccagactgct gtgaaatccg ttgccaacag ccaggatatc gatctggttg
    42361 ttgatgcaaa cgccgttgct tacaacagca gcgatgtaaa agacatcact gccgacgtac
    42421 tgaaacaggt taaataagta atgccttcaa ttcgactggc tgatttagcg cagcagttgg
    42481 atgcagaact acacggtgat ggcgatatcg tcatcaccgg cgttgcgtcc atgcaatctg
    42541 cacaaacagg tcacattacg ttcatggtta acccaaaata ccgtgagcat ttaggcttgt
    42601 gccaggcgtc cgcggttgtc atgacccagg acgatcttcc tttcgcgaaa agtgccgcgc
    42661 tggtagtgaa gaatccctac ctgacttacg cgcgtatggc gcaaatttta gataccacgc
    42721 cgcagcccgc gcagaacatt gcacccagtg cggtgattga cgcgacggcg aagctgggta
    42781 acaacgtatc gattggcgct aacgcggtga ttgagtccgg cgttgaactg ggcgataacg
    42841 tgattatcgg tgccggttgc ttcgtaggta aaaacagcaa aatcggtgca ggttcgcgtc
    42901 tctgggcgaa cgtaaccatt taccatgaga tccagatcgg tcagaattgc ctgatccagt
    42961 ccggaacagt ggtcggcgca gacggctttg gttatgccaa cgatcgtggt aactgggtga
    43021 agatcccaca gattggtcgc gtaattattg gcgatcgcgt ggagatcggt gcctgcacta
    43081 ccattgatcg tggcgcgctg gatgacactg ttattggcaa tggtgtgatc attgataacc
    43141 agtgccagat tgcacataac gtcgtgattg gcgacaatac ggcagttgcc ggtggcgtca
    43201 ttatggcggg cagcctgaaa attggtcgtt actgcatgat cggcggagcc agcgtaatca
    43261 acgggcatat ggaaatatgc gacaaagtga cggttacggg catgggtatg gtgatgcgtc
    43321 ccatcactga accaggcgtc tattcctcag gcattccgct gcaacccaac aaagtctggc
    43381 gcaaaaccgc tgcactggtg atgaacattg atgacatgag caagcgtctg aaatcgcttg
    43441 agcgcaaggt taatcaacaa gactaacgtt ccatcttttg ttcgccaaac tttacggcct
    43501 gtctcattct tacgattgcg gcaggccgtg ttattattgt cgtttcttat attttgacag
    43561 gaagagtatc ttgactacta acactcatac tctgcagatt gaagagattt tagaacttct
    43621 gccgcaccgt ttcccgttct tactggtgga tcgcgtgctg gattttgaag aaggtcgttt
    43681 tctgcgcgca gtaaaaaatg tctctgtcaa tgagccattc ttccagggcc atttccctgg
    43741 aaaaccgatt ttcccgggtg tgctgattct ggaagcaatg gcacaggcaa caggtattct
    43801 ggcgtttaaa agcgtaggaa aactggaacc gggtgagctg tactacttcg ccggtattga
    43861 cgaagcgcgc tttaagcgcc cggtcgtacc tggcgatcaa atgatcatgg aagtcacttt
    43921 cgaaaaaacg cgccgcggcc tgacccgttt taaaggggtt gctctggtcg acggtaaagt
    43981 agtttgcgaa gcaacgatga tgtgtgctcg tagccgggag gcctgatacg tgattgataa
    44041 atccgccttt gtgcatccaa ccgccattgt ggaagagggc gcgtcgattg gcgcgaacgc
    44101 tcacattggt cctttttgta tcgttggacc ccatgtcgaa attggtgagg gtaccgtact
    44161 gaaatctcac gttgtcgtga atggtcatac taaaattggc cgcgataatg agatttatca
    44221 gttcgcctcc atcggcgaag ttaaccagga tctgaaatat gctggcgaaa cgacccgcgt
    44281 ggaaatcggc gatcgtaacc gcattcgcga aagcgtcacc attcatcgtg gcacagtcca
    44341 gggcggtgga ttgacgaagg tgggcagcga caacttactg atgatcaatg cgcacattgc
    44401 gcacgattgt acggtaggta accgctgcat cctcgccaac aacgcaacgc tggcgggtca
    44461 cgtatcggtt gacgacttcg cgatcatcgg aggcatgacc gcagtccatc agttctgcat
    44521 cattggtgcg catgtgatgg ttggcggctg ctccggtgtg gcgcaggacg tccctcctta
    44581 tgtcattgcg cagggtaacc acgcaacgcc gttcggtgtc aatatcgaag ggctgaagcg
    44641 ccgcggattc agccgtgagg cgattaccgc tatccgtaat gcgtataagc tgatttatcg
    44701 tagcggtaaa acgctcgatg aagtgaaacc ggaaattgct gaactggcgg aaacatatcc
    44761 ggaagtgaaa gcctttaccg atttctttgc acgctcaacg cgcggtctga ttcgttaatg
    44821 actgaacagc gtccattaac gattgccctg gtcgccggag aaacctccgg cgatatcctg
    44881 ggggccggtt taatccgcgc tctgaaagaa cgtgtgccca acgcccgctt tgttggtgtt
    44941 gccgggccac gaatgcaggc tgaaggctgc gaagcctggt acgaaatgga agaactggca
    45001 gtgatgggca ttgttgaagt gctcggtcgt ctgcgtcgct tactacatat tcgtgccgat
    45061 ctgacaaagc gttttggcga actgaagcca gatgtttttg ttggtattga tgcgcctgac
    45121 ttcaatatta ctcttgaagg taacctcaaa aagcagggta tcaaaaccat tcattacgtc
    45181 agcccgtccg tctgggcgtg gcgacagaaa cgcgttttca aaataggcag agccaccgat
    45241 ctggtgctcg catttctgcc tttcgaaaaa gcgttttatg acaaatacaa cgtaccgtgc
    45301 cgctttatcg gtcataccat ggctgatgcc atgccattag atccagataa aaatggtgcc
    45361 cgtgatgtgc tggggatccc ttacgatgcc cactgtctgg cattgttgcc gggcagccgt
    45421 ggtgcagaag tcgaaatgct tagtgccgat ttcctgaaaa cggcccagct tttgcgccag
    45481 acatatccgg atctcgaaat cgtggtgccg ctggtgaatg ccaaacgccg cgagcagttt
    45541 gaacgcatca aagctgcagt cgcgccagac ctgtcggttc atttgctgga tggaatgggc
    45601 cgtgaggcga tggtcgccag tgatgcggcg ctactggcgt cgggtacggc agcactggag
    45661 tgtatgctgg ctaaatgccc gatggtagtg ggatatcgca tgaagccttt caccttctgg
    45721 ctggcgaagc gactggtgaa aactgattat gtctcgctgc caaatctgct ggcgggcaga
    45781 gagttagtca aagagttatt gcaggaagag tgtgagccgc aaaaattggc tgcggcgctg
    45841 ttaccgctgt tggcgaacgg gaaaaccagc catgcgatgc acgacacctt ccgcgagctg
    45901 catcagcaga tccgctgcaa tgccgatgag caggcggcac aagccgttct ggagttagca
    45961 caatgatcga atttgtctat ccgcacacgc agctggttgc gggtgtggat gaagtcggac
    46021 gcgggccgtt agttggcgcg gtcgtcaccg ctgcggtgat ccttgacccg gcgcgcccaa
    46081 ttgccgggct gaatgattcc aaaaagctga gcgaaaaacg ccgtctggcg ctctgtgaag
    46141 agatcaaaga gaaagcgttg agctggagtc tgggccgcgc ggaacctcac gaaatcgacg
    46201 aactgaatat tttacatgcg accatgctgg cgatgcagcg tgccgtcgct ggactgcata
    46261 ttgcgccgga atatgtgttg attgatggta accgctgccc gaaattaccg atgccttcga
    46321 tggctgtggt gaaaggcgat agccgcgtac cggaaatcag tgccgcgtct atcctggcga
    46381 aagtgacgcg tgacgccgaa atggcggcgc tggatattgt tttcccgcaa tatggttttg
    46441 cccaacacaa agggtatcca accgcttttc atctggaaaa actggctgaa cacggcgcga
    46501 ccgaacacca tcggcgcagc tttgggcctg tcaaacgcgc actgggactt gcgtcctgat
    46561 tcttgtgtcg agattaagta aaccggaatc tgaagatgtc tgaaccacgt ttcgtacacc
    46621 tgcgggtgca cagcgactac tcgatgatcg atggcctggc caaaaccgca ccgctggtaa
    46681 aaaaggcggc ggcgttgggt atgccagcac tggcgatcac cgatttcacc aacctttgcg
    46741 gtctggtgaa gttctacgga gcgggacatg gcgcagggat taagcccatc gtcggggcgg
    46801 attttaacgt ccagtgcgac ctgctgggtg atgagttaac ccacctgacg gtgctggcgg
    46861 cgaacaatac cggctatcag aatctgacgt tgctgatctc aaaagcgtat cagcgcgggt
    46921 acggtaccgc cgggccgatc atcgatcgcg actggcttat cgaattaaat gaagggttga
    46981 tccttctttc cggcgggcgc atgggcgacg ttggacgcag tcttttgcgt ggtaacagcg
    47041 cgctggtaga tgagtgtgtc gcgttttatg aagaacactt cccggatcgc tattttctcg
    47101 agctgatccg caccggcagg ccggacgaag aaagctatct gcacgctgcg gtggaactgg
    47161 cggaagcgcg cggtttgccc gtcgtggcga ccaatgacgt gcgctttatc gacagcagcg
    47221 actttgacgc gcacgaaatc cgcgtcgcga tccatgacgg ctttactctc gacgatccta
    47281 aacgcccgcg taactattcg ccgcagcaat atatgcgtag cgaagaggag atgtgcgagc
    47341 tttttgccga cattcccgaa gcccttgcca acaccgttga gatagccaaa cgttgtaacg
    47401 taactgtgcg tctgggtgaa tacttcctgc cgcaattccc gaccggggac atgagtaccg
    47461 aagattatct ggtcaagcgt gcaaaagaag gcctggaaga gcgtctggca tttttattcc
    47521 ctgacgagga agaacgtctt aagcgtcgtc cggagtatga cgaacgtctg gatactgaac
    47581 ttcaggttat caaccagatg ggcttcccgg gttacttcct catcgttatg gaatttatcc
    47641 agtggtcgaa agataacggt gtaccggtag ggccaggccg tggttctggt gcagggtcac
    47701 tggtggccta cgcgttaaaa atcaccgatc tcgatccgct ggaatttgac ctgctgttcg
    47761 aacgtttcct taacccggaa cgtgtctcta tgcctgactt cgacgttgac ttctgtatgg
    47821 agaaacgtga ccaggttatc gaacatgtgg cggacatgta cggtcgcgat gcggtatcgc
    47881 agattatcac cttcggtacg atggcggcga aagcggtgat ccgcgatgta ggccgtgtat
    47941 tggggcatcc gtacggattt gtcgatcgta tctcgaaact gatcccgccc gatccgggta
    48001 tgacgctggc gaaagcgttt gaagccgagc cgcagctgcc ggaaatctac gaagcggatg
    48061 aagaagttaa ggcgctgatc gacatggcgc gcaaactgga aggggtcacc cgtaacgccg
    48121 gtaagcacgc cggtggggtg gttatcgcac cgaccaaaat taccgatttt gcgccgcttt
    48181 actgcgatga agagggcaaa catccggtca cccagtttga caaaagcgac gttgaatacg
    48241 ccgggctggt gaagttcgac ttcctcggtt tgcgtacgct caccatcatc aactgggcgc
    48301 tggagatgat caacaagcgg cgggctaaga atggcgagcc gccgctggat atcgccgcga
    48361 tcccgctgga tgacaagaaa agcttcgaca tgctgcaacg ctcggaaacc acggcggtat
    48421 tccagcttga atcgcgcggc atgaaggacc tgatcaagcg tctgcaacct gactgcttcg
    48481 aagatatgat cgcactggtg gcactgttcc gccccggccc gttgcaatca gggatggtgg
    48541 ataactttat cgaccgtaaa catggtcgcg aagagatctc ctatccggac gtgcagtggc
    48601 agcatgaaag cctgaaaccg gtactggagc caacctacgg catcatcctg tatcaggaac
    48661 aggtcatgca gattgcccag gtgctttctg gttataccct cggtggcgcg gatatgctgc
    48721 gtcgtgcgat gggtaagaaa aagccggaag agatggctaa gcagcgttct gtatttgctg
    48781 aaggtgcaga aaagaacgga atcaacgccg aactggcgat gaaaatcttc gacctggtgg
    48841 agaaattcgc tggttacgga tttaacaaat cgcactctgc ggcctatgct ttggtgtcat
    48901 atcaaacgtt atggctgaaa gcgcactatc cggcggagtt tatggcggcg gtaatgaccg
    48961 ccgatatgga caataccgag aaggtggtgg gcttggtgga tgagtgctgg cggatggggc
    49021 tgaaaatcct accgccggat ataaactccg gtctttacca tttccacgtc aacgacgacg
    49081 gcgaaatcgt gtatggtatt ggcgcgatca aaggggtagg tgaaggcccg attgaggcca
    49141 tcatcgaagc ccgcaataaa ggtggctact tccgcgaact gtttgatctc tgcgcccgta
    49201 ccgacaccaa aaagttaaac cggcgagtgc tggaaaaact gatcatgtcc ggggcgtttg
    49261 accgtcttgg gccacaccgc gcggcgctga tgaactcgct gggcgatgcg ttaaaagcgg
    49321 cagatcaaca cgcgaaagcg gaagctatcg gtcaggccga tatgttcggc gtgctggccg
    49381 aagagccgga acaaattgaa caatcctacg ccagctgcca accgtggccg gagcaggtgg
    49441 tattagatgg ggaacgtgaa acgttaggtc tgtacctgac gggacaccct atcaaccagt
    49501 atttaaaaga gattgagcgt tatgtcggag gcgtaaggct gaaagacatg cacccgacag
    49561 aacgtggtaa agtcatcacg gctgcggggc tcgttgttgc cgcgcgggtt atggtcacca
    49621 agcgcggcaa tcgtatcggt atctgcacgc tggatgaccg ttccgggcgg ctggaagtga
    49681 tgttgtttac tgacgccctg gataaatacc agcaattgct ggaaaaagac cgcatactta
    49741 tcgtcagcgg acaggtcagc tttgatgact tcagcggtgg gcttaaaatg accgctcgcg
    49801 aagtgatgga tattgacgaa gcccgggaaa aatatgctcg cgggcttgct atctcgctga
    49861 cggacaggca aattgatgac cagcttttaa accgactccg tcagtctctg gaaccccacc
    49921 gctctgggac aattccagta catctctact atcagagggc ggatgcacgc gcgcgattgc
    49981 gttttggcgc gacgtggcgt gtctctccga gcgatcgttt attaaacgat ctccgtggcc
    50041 tcattggttc ggagcaggtg gaactggagt ttgactaata caggaatact atgagtctga
    50101 atttccttga ttttgaacag ccgattgcag agctggaagc gaaaatcgat tctctgaccg
    50161 cggttagccg tcaggatgag aaactggata ttaacatcga tgaagaagtg catcgtctgc
    50221 gtgaaaaaag cgtagaactg acgcgtaaaa tcttcgccga tctcggtgca tggcagattg
    50281 cgcaactggc acgccatcca cagcgtcctt ataccctgga ttacgttcgc ctggcatttg
    50341 atgaatttga cgaactggct ggcgaccgcg cgtatgcaga cgataaagct atcgtcggtg
    50401 gtatcgcccg tctcgatggt cgtccggtga tgatcattgg tcatcaaaaa ggtcgtgaaa
    50461 ccaaagaaaa aattcgccgt aactttggta tgccagcgcc agaaggttac cgcaaagcac
    50521 tgcgtctgat gcaaatggct gaacgtttta agatgcctat catcaccttt atcgacaccc
    50581 cgggggctta tccgggcgtg ggcgcagaag aacgtggtca gtctgaagcc attgcacgca
    50641 acctgcgtga aatgtctcgc ctcggcgtac cggtagtttg tacggttatc ggtgaaggtg
    50701 gttctggtgg tgcgctggcg attggcgtgg gcgataaagt gaatatgctg caatacagca
    50761 cctattccgt tatctcgccg gaaggttgtg cgtccattct gtggaagagc gctgacaaag
    50821 cgccgctggc ggctgaagcg atgggtatca tcgctccgcg tctgaaagag ctgaaactga
    50881 tcgactccat catcccggaa ccgctgggcg gtgctcaccg taacccggaa gcgatggcgg
    50941 cgtctctgaa agcgcagctg ctgacggatc tggccgatct cgacgtgtta agcactgaag
    51001 atttaaaaaa tcgtcgttat cagcgcctga tgagctacgg ctacgcgtaa tcggcaaatg
    51061 ttctgaaaag ggtcacttcg gtggcccttt tttatcgcca cggtttgagc aggctatgat
    51121 taaggaagga ttttccagga ggaacacatg aacatcattg ccattatggg accgcatggc
    51181 gtcttttata aagatgagcc catcaaagaa ctggagtcgg cgctggtggc gcaaggcttt
    51241 cagattatct ggccacaaaa cagcgttgat ttgctgaagt ttatcgaaca taacccacga
    51301 atttgcggcg tgatttttga ctgggatgag tacagtctcg atttatgtag cgatatcaat
    51361 caacttaatg aatatctccc gctttatgcc ttcatcaaca cccactcgac gatggatgtc
    51421 agcgtgcagg atatgcggat ggcgctctgg ttttttgaat atgcgctggg gcaggcggaa
    51481 gatatcgcca ttcgtatgcg tcagtacacc aacgaatatc ttgataacat tacgccgccg
    51541 ttcacgaaag ccttgtttac ctacgtcaaa gagcggaagt acaccttttg tacgccgggg
    51601 catatgggcg gcaccgcata tcaaaaaagc ccggttggct gtctgtttta tgattttttc
    51661 ggcgggaata ccctcaaggc ggacgtctct atttcggtca ccgagcttgg ttcgttgctc
    51721 gaccacaccg ggccacacct ggaagcggaa gagtacatcg cgcggacgtt tggcgcggaa
    51781 cagagttata tcgttaccaa cggaacatcg acgtcgaaca aaattgtggg tatgtacgcc
    51841 gcgccatccg gcagtacgct gttgatcgac cgcaattgtc ataaatcgct ggcgcatctg
    51901 ttgatgatga acgatgtagt gccagtctgg ctgaaaccga cgcgtaatgc gttggggatt
    51961 ctgggtggga tcccgcgcgg tgaatttact cgcgacagca tcgaagagaa agtcgctgcc
    52021 accacgcagg cacaatggcc ggttcatgcg gtgatcacca actccaccta tgatggcttg
    52081 ctctacaaca ccgactggat caaacagacg ctggatgtcc cgtcgattca cttcgattct
    52141 gcctgggtgc cgtacaccca ttttcatcca atctaccagg gtaaaagtgg tatgagcggc
    52201 gagcgtgttg caggaaaagt gatcttcgaa acgcagtcga cccacaaaat gctggcggcg
    52261 ttatcgcagg cgtcgctgat ccacattaaa ggtgagtatg acgaagaggc gtttaacgaa
    52321 gcctttatga tgcataccac cacctcgccc agttatccca ttgttgcttc tgttgagacg
    52381 gcggcggcga tgttgcgtgg taatccgggc aaacggctga ttaaccgttc agtagaacga
    52441 gctctgcatt ttcgcaaaga ggtccagcgg ctgcgggaag agtctgacgg ctggtttttc
    52501 gatatctggc aaccgccgca ggtggatgaa gccgaatgct ggcccgttgc gcctggcgaa
    52561 cagtggcacg gctttagcga tgcggatgcc aatcacatgt ttctcgatcc ggttaaagtc
    52621 actattttga caccggggat ggacgagcag ggcaatatga gcgaggaggg gatcccggcg
    52681 gcgctggtgg caaaattcct cgacgaacgt gggatcgtag tagagaaaac cggcccgtat
    52741 aacctgctgt ttctctttag tattggcatc gataaaacca aagcaatggg attattgcgt
    52801 gggttgacgg aatttaagcg ctcttacgat ctcaacctgc ggatcaaaaa tatgctgccc
    52861 gatctctatg cagaagatcc cgatttctac cgcagtatgc gtattcagga tctggcgcaa
    52921 gggatccata agctgattcg taaacacgat cttcccggtt tgatgttgcg ggcatttgat
    52981 accttgccgg agatgatcat gacgccacat caggcatggc agagacagat taaaggcgaa
    53041 gtagaaacca ttgcgctgga acaactggtc ggtagagtat cggcaaatat gatcctgcct
    53101 tatccacccg gcgtaccgct gctgatgccg ggagaaatgc ttaccgaaga gagccgcacg
    53161 gtactcgatt ttctactgat gctttgttcc gtagggcaac attaccccgg ttttgaaacg
    53221 gatattcacg gcgcgaaaca ggacgaagac ggcgtttacc gcgtacgagt cctaaaaatg
    53281 gcgggataac ttgccagagc ggcttccggg cgagtaacgt gctgttaaca aataaaggag
    53341 acgttatgct gggtttaaaa caggttcacc atattgcgat tattgcgacg gattatgcga
    53401 tgagcaaagc tttctactgc gatattcttg gtttcacgct gcaaagcgaa gtctatcgcg
    53461 aagcgcgcga ctcgtggaaa ggggatttgg cgcttaatgg gcaatatgtg attgagcttt
    53521 tctcatttcc gttcccgccg gagcgcccca gccgaccgga agcttgcggc ctgcgtcatc
    53581 tggcttttag cgttgatgat atcgatgcgg cagtggcgca ccttgaaagc cataacgtga
    53641 agtgtgaagc catccgtgtc gatccataca cgcaaaaacg cttcacgttc tttaacgatc
    53701 cggacgggct gccgttggaa ctgtatgagc agtaaggctt gtcatcgccg catttgcccg
    53761 gtaacgtgcc gggcattgct actgtaaaat cgcaccatca tgacactcac actgaataca
    53821 caacttctct cctcacgcca gattctggtg gcctttagcg gcggccttga ttctactgtc
    53881 ttactgcatc aactggtgca gtggcggacg gaaaatccgg gcgtcactct gcgcgctatc
    53941 catgtgcatc acggtttaag cgccaatgct gatgcctggg tgaaacattg tgaaaacatc
    54001 tgccaacagt ggcaggtgcc gctggtggtc gaacgtgtgc agcttgcgca agaggggcta
    54061 ggtattgagg cccaggcgcg gcaggcacgt tatcaggcat ttgcccgcac cttgttgccc
    54121 ggtgaagtgc tggttacagc gcaacatctc gacgatcaat gtgaaacctt tctgctggcg
    54181 ctaaaacgcg gtagcggccc tgccgggctt tcggctatgg cggaagtttc ggagtttgcc
    54241 ggaacacggc ttatccgccc gttgcttgcc cgcacgcggg gggaactgga gcagtgggcg
    54301 cgtcagtatg atttacgctg gattgaagac gaaagtaatc aagacgattc ctacgatcgt
    54361 aacttcctgc gcctgcgcgt agtgccgtta ttgcagcagc gttggccgca ttttgccgaa
    54421 gcgacggccc gcagcgccgc actttgcgcc gaacaagaga gcctgctgga tgaactgctg
    54481 gcggatgatt tagcgcactg ccaaacgcaa cagggaacgt taaagattgc gccaatgctg
    54541 gcaatgagcg atgcccgtcg cgcggcgatt atccgccgct ggctggcagg gcagaatgca
    54601 ccgatgcctt cccgcgaggc gctggtgcgg atctggcagg aagtggcgct ggcgcgggaa
    54661 gatgcttccc cctgtttacg tttgggcgcg tttgaaatcc gccgctatca gtcgcaactg
    54721 tggtggatta aatccgttgc cgggcaaagc gaaaccattg tgccgtggca gacgtggcta
    54781 caaccgctgg aattaccggc ggggctggga agtgtacagc ttactgcggg aggcgatatt
    54841 cgccctccgc gtgcagacga agcggtcagc gtgcgtttca aagcgccagg actgctgcat
    54901 attgtcgggc gcaacggtgg acgtaagctg aagaaaatct ggcaagagtt aggcgttccg
    54961 ccgtggctac gtgacaccac gccactgctg ttttatggcg aaacgctgat tgcggcggca
    55021 ggggtatttg tgacgcaaga aggtgtggct gtaggtgaga atggcgtcag ttttgtctgg
    55081 cagaaaacgc ttagtcaagt gaaagccgga taagacgcat caaacgtcgc atccggcgaa
    55141 agtcaatcag gactcgctca ccaccaccgt accgatttcc gggtggctaa agctggtaat
    55201 tttatccaga cgcagctcgc gggttgcacc agcggcctcg acgaccagat actccacatt
    55261 tttgcgggag actaaatcgc tggccttcgc ctgcaatttt tcgccatctt tcagctcaag
    55321 tgtcagcatt aaatgatgct ggcaggcgag ctcaagatta tcgtaatcat cacaattgat
    55381 tggttgatac gtatcattca ttgacataat cgctcaccag taagtttgcc gcagcgtatg
    55441 ctgctttttc cctgacagcc tcagaaaggg tgtcgtcggc agccatttca ttcagcactt
    55501 tcaaaacgca gcccagcgcg tccggaacgt atcctaagtc tccgctggcg atttccgcgt
    55561 accgcttgcg tattaactca caatattttt ccacatgccc tcctgtcagc actctgactt
    55621 aaccgtggat gcaagtctaa gcctacgaag ataaactctg tttcgcaagg tgactatacc
    55681 acactcattt ctgcaatatc agcgccgcaa ctgcacgtat tccgttacaa tggcctcctg
    55741 attcgaaagg agttttctta tggcgcttaa agcgacaatt tataaagcga cggttaatgt
    55801 ggccgatctc gaccgcaacc agtttctcga tgcctctctg acgctggcgc gccatccttc
    55861 agaaacccag gagcgcatga tgctgcgcct gctggcgtgg ctaaaatatg ccgatgaacg
    55921 tctgcaattt acccgtggtt tgtgtgccga tgatgagccg gaagcgtggc tgcgtaacga
    55981 tcatttgggt attgatttgt ggattgagct ggggctgccg gatgagcgac ggattaagaa
    56041 agcctgcacc caggccgcag aagtggcgct gtttgcctat aatagtcggg cggcgcaaat
    56101 ctggtggcag caaaatcaga gcaaatgcgc gcagttttcc aatctttcca tctggtatct
    56161 ggacgatgaa caactggcga aagtgagcgc ctttgccgat cgtaccatga cgctgcaggc
    56221 aacgattcag gatggcgtga tctggttatc ggatgataag aataatctgg aagtgaactt
    56281 aaccgcctgg caacaacctt catgattgtg atttcccgac atgttgctat ccccgatggt
    56341 gagcttgaga tcaccgccat tcgtgcgcag ggcgcgggcg ggcagcatgt taataagacc
    56401 tcaacggcta ttcatctgcg ttttgacatt cgggcgtcca gcctgccaga gtattacaaa
    56461 gaacgtctgc tcgccgccag ccatcacttg atcagcagtg atggcgtgat tgtcattaag
    56521 gcacaggaat accgcagtca ggaactgaac cgcgaagcgg ctctggcccg gctggtggca
    56581 gtgattaaag atttaacaac agaacaaaaa gcccgacgac ccacgcgccc cacccgtgca
    56641 tcgaaagagc gcaggctggc atcgaaagca caaaaatcaa gcgtgaaggc gatgcgcggc
    56701 aaagtgcgca gcggtcggga ataaaaagaa ggaatggatg gtgaaaaaag cgatagtgac
    56761 agcgatggct gtaatcagcc tctttactct gatgggttgt aataatcggg ccgaagtcga
    56821 tacgctttct ccggcgcagg ctgccgaact gaaaccgatg ccgcaaagtt ggcgcggcgt
    56881 gctgccgtgt gctgattgcg aaggaatcga aacctctctg ttcctcgaaa aagacggaac
    56941 atgggtgatg aatgagcgtt atctcggtgc tcgtgaagaa ccttcctcct tcgcttccta
    57001 cggtacatgg gcgcgaaccg ctgacaagct agtattaacc gacagcaaag gtgaaaagtc
    57061 atattatcgg gcgaaaggcg atgcgctgga gatgctcgat cgtgaaggca atccgattga
    57121 atcgcagttc aactatacgc tagaaccggc acaatccagt ttacctatga cgccgatgac
    57181 cctgcggggc atgtattttt atatggctga tgcggcgacc ttcactgatt gcgcgaccgg
    57241 aaaacgtttc atggtagcga ataacgcaga gctggagcgt agctacctgg ctgcgcgcgg
    57301 tcacagtgaa aaaccggtgt tattgtcagt agaaggtcac tttacgcttg agggtaatcc
    57361 ggataccggt gcgccgacta aagtattggc acccgatacg gcgggtaaat tttatccgaa
    57421 ccaggattgc agtagtttgg ggcagtaatt gaaagccgga gcattctccg gcttttatca
    57481 ctgagtcagc ccaacaaagc gacccgcctt gatataaatc cctggcttca tgtgcccaac
    57541 atactgtaac tgcgtttctg gcttaaacgc agagatatcg ccagtacgca tatgcatcag
    57601 atcggcaggg ctaatccagc ctgattgcgc cagcgtaagc ggatgcccgg ctttggcaaa
    57661 tgcatcagtg acaaattccg aacaaaacca cgactttttg tctccttcgc ccacactgct
    57721 taactgcgct ttcgccaggc cgctgacgca ctgttgacga aaatcctcgg agaacggatt
    57781 cagtgagcac acctggcgag tcaccataaa gggaataaat tcgacaatgc cgcgatagtt
    57841 ataaccgcta tctttgattt tattagcaaa cgcggtgata tctgtggctt gttgcggggt
    57901 aagatccggg actcgtaaga cgaaaagctt atcactgtgc ttcatcgctt ttttaaggga
    57961 aacgatctgg acgccagcgc ctgtcgcttc tgcaacgtta ttatcaccca gaaagattgc
    58021 aacgtgactc acagaggaag tgctgaagac gcggattcca aatgaggtta ccccaaggct
    58081 tgaggagaac agcaaatcgc cgggtttgag atcaggtgcc gttatttctt ttattgattg
    58141 ttcggtgaaa gagctttgat gctggaattt aacagcccat gttttcgcct cggcatttac
    58201 cactgtggct gatgagtctg gctggctgat atcaacggta caggccgaaa gtaaaagaaa
    58261 gcaggggaga agcaggcggc agtacgcctt tggtttatcc attttataca atccatgtaa
    58321 aaaagggccc cgaaattcag gaccctttct ggcatcagcc tttaatctgt ttcaccagat
    58381 aatcgacgat gtcaccagtc ttaattaact gtttctcgcc gttacgacga tatttatatt
    58441 cgatatcatc gttgtcgagg ttacggtcgc ccagcacaat agtgtgcgga ataccgatca
    58501 gttccatatc agcaaacatc acgcccggac gctctttgcg gtcatccagc agcacttcga
    58561 taccttgtgc gcgcagttcg ctgtacagtt tctcagcaag ttcctgtacg cggaaggatt
    58621 tgtgcatgtt cattggcaga atcgccacct ggaacggcgc gatagcgtca ggccatacga
    58681 tgccgcgttc gtcgtagttc tgctcaatcg ccgctgctac cacacgcgtt acgccgatac
    58741 cgtagcaacc catcgtcagg atttggttac ggccatcttc accctgtacg gaggctttca
    58801 gtgcttcgga gtacttggta cccagctgga agatgtgacc gacttcgata ccacgtttga
    58861 tcagcagcgt accctgacca tccgggcttg gatcgccagc caccacgttg cggatatcag
    58921 caatttccgg ggtagcgaca tcgcgatccc agttgatacc gaagtagtgt ttaccatcga
    58981 tgttagcacc agcagcgaaa tcactcatcg ccgcaacggt acggtcaatc accaccggaa
    59041 tcggcatgtt taccggaccc agtgaacccg gaccggcttt aaccacggca cgaatttctt
    59101 cttcggtcgc gaaagtcagc gggcttgcaa cctgcggcag tttttctgct ttaacttcgt
    59161 tcagctcgtg gtcaccgcgc accagcagcg caaccagcgg gaagctactg ccttcaaccg
    59221 ctttaaccag cagggtctta accgttttct caatcggcag attgaactgt tcaaccagtt
    59281 ccgcgatggt cttcgcgttc ggcgtatcaa ccagcgtcat ttcctgggta gcagcagcgc
    59341 gcggttcttt cggcgcgata gcttctgcca gttcaatgtt cgctgcatag tcagaggtgt
    59401 cggagaagac cacatcgtct tcaccgcttt gcgccagcac ctggaattcg tgagaggcgc
    59461 taccgccgat agaaccggta tcggcctgta cggcgcggaa atccagcccc atgcggctga
    59521 agattttgct gtaggccgca tacattgcat cgtaggtttc ctgcagggat tcctgagagg
    59581 tatggaaaga gtaagcatct ttcatcagga attcgcggga acgcatgacg ccgaaacgcg
    59641 ggcgcacttc atcacggaac ttagtctgga tctgatagaa gttcagcggc agctgtttgt
    59701 aagagctcag ctcgttacga atcaggtcgg taatcacttc ttcatgagtt gggccaagta
    59761 cgaacggacg gtcaccacgg tcagcaatac gcagcaattc cgggccatac tgttcccaac
    59821 gaccactctc ttgccacagt tcagatggct gaaccaccgg cattaacacc tcaatcgcac
    59881 cggcgttgtt catctcttca cgcacgatgt tttcgacttt tttcagaacg cgcacgccgg
    59941 tcggcagcca ggtgtataac ccggaggcca gcttgcggat catcccggcg cgcagcatca
    60001 gctgatggct gatcacctcg gcgtcggcag gtgtctcctt gagagtggag agcaggtatt
    60061 ggctagtacg catgttgtta cggttccagt tggaaggtag aacaggctca aggtgagcct
    60121 gggacaaaaa aagtgattta gtttaccagt gcaaaagaaa tgtcaaaaga gaagggcgtg
    60181 aatttaacgc ggttccagcg caaagacttc aaaacctgcg tcggtgacgc gccagcgaac
    60241 gttaaaatca tgcagccaga cggcataggt tttgcccgtt tcctcacctt tacgataggc
    60301 cgggcgcggg tcctgcgcta gtacttcgcg gataaacagc gttaactgcg gatagcgctt
    60361 ctccagcgtc aaaagctgct tttcgacctc tgcggtaaaa ctcaccgcca tctctgcagc
    60421 tggcgcactt tgcgcatagc tggcactggc atttggaagc gattcggcaa aggggagata
    60481 cggtttgata tccactaccg gcgtaccatc gaccagatcc agactgccga gcttcagaat
    60541 cacgctgtct ttatggcaaa caacctcttt cagctctacc agcgacatac caattgggtt
    60601 agggcgaaaa gtggagcgtg ttgcgaaaac ccccattctg gcattaccgc caagacgagg
    60661 cggacgcaca gtcggacgcc agccgccttc catcgtttga tgaaagacga aaagtatcca
    60721 taaatggctg aacgcttcca ggccgcgaac ggcgtcggct tggttgtagg gagcaatgag
    60781 atgcagttcg ccgttggcgc tttttaccag acctggttgg cgcggaacgg cgaatttttc
    60841 tttatagggc gagcgaataa cgcctatttg ctcgaactgg aaactgctca tttcgccgta
    60901 atgttaagcg cagaaccgat acatacagcc tgacgatagc agcctggcgt accgctggtg
    60961 acttcgcagc tatgcagtaa taccgcattg gctttcattt tcgaggcgtt gatttgcatc
    61021 cgcttacgcg cggttggaat gctcggcgga gagtcctgat tagaggcctg gcaagagtcg
    61081 ccactgactt caccgagatc gcggaacggt ttgccgacta attcttctgc attggtataa
    61141 attcggaccg gcgtggcgcg cggcactttc ggttttgcag actccgcttt cggctggggt
    61201 gcagtgcttt gaacgggttc gacaggggat ctgcttaaca tggaacagcc gcttagcatg
    61261 agtgctacta aacagatcgg taaagcacgc atagtatttc ctcaatgtat gatcaaaacg
    61321 tcaatattga atcaggagct tgtaaaaatg acaagacggg caagcgcccg tcctgaatga
    61381 tattacaaat tgtggaaaca gcctaaaaat taccagcctt taacagcgcc gccgttaaac
    61441 actttgtttg ctgcttcgta aacttcgtca gactgataag cctggacgaa tttcttcacg
    61501 ttctcggcgt ctttgttatc ttcacgcgtt acgatcaggt ttacgtacgg ggactcttta
    61561 tcttcgacaa agataccgtc tttcgccgga gtcaggccaa tctggctggc ataggtggtg
    61621 ttaataactg ccagagcgat ttgcgcgtcg tccagagaac gcggcagctg cggtgcttcc
    61681 agctcaacaa ttttcagatt tttggggttc tcaacaacat caagaacggt cggcagcagg
    61741 ccaacgccat ctttcagttt gatcaagccc actttttgca gcagcagcag tgaacgacca
    61801 aggttagttg ggtcgtttgg cacggcaacc tgcgaaccat cctgcagttc atccagtgat
    61861 ttgattttct tggagtaacc tgcaatcgga taaacaaaag tgttgcctac tgcgaccagt
    61921 ttgtagccac gatctttcag ttgctgatca aggtacggtt tatgctggaa ggcgttggcg
    61981 tcgatatcgc ctttgctcaa tgcttcgttt ggcagaacat agtcgttgaa ggttaccagc
    62041 tcaacgtcca ggccatattt gtctttcgca actttctgcg cgacttctgc aacctgctgt
    62101 tcggcaccaa caatcacgcc gactttaatg tggtttggat ctttttcatc ctgaccgcag
    62161 cctaccagtg ccagtgatcc aatcagggct cccactgccg caaaggtttt gaatttgaac
    62221 gccatacctt attccttctt caattattta tgttgtgttg aacgttactt gcgagtgaca
    62281 gcccggacga tgcggtcgcc tgcgaactga attaaataaa ccagaatgac cagcaatacc
    62341 agtaccgtat tcatcaccgt cgcgttgtag ccgatgtagc catactgata gccaatctga
    62401 cctaaaccac cggcaccgac tgcaccaccc atcgcggaat aaccgaccag ggtaatcagg
    62461 gtgatagttg ccgcatttac cagacccggc aacgcttccg gtaacagcac tttacggacg
    62521 atctgcattg gcgtggcacc cattgcgcgg gaagcttcaa ttaacccggt tgggatctcc
    62581 agcagagcgt tctcgaccat acgggcaata aacggtgctg caccaacggt taacggaaca
    62641 atcgctgcct gcaaaccaat cgatgtacca acaataacgc gggtaaacgg aatcatccat
    62701 accagcaaga taatgaacgg gatggaacgg aaaatgttca caatcgcaga aacggtacga
    62761 tacagcttcg cgttagcaat aatttgcccc ggacgcgtga cataaagcag gacgccaacc
    62821 ggcagaccaa tcacaaatcc aaaaaaaccg gatacgaagg tcattgccag cgtttcccat
    62881 acgccacgaa ccagcagcca catcatcggc tcagacataa cccagtacct ctacttttac
    62941 atggtgttcc tgcagccagg taatggcggc ttgcgtatct tgttgtgtgc cgtgcatttc
    63001 agtcagcatg atgccgaact taacgccacc ggcgtaatcc atctgcgcgc taataatgtt
    63061 gttgttgacg ttgaaacgac gcgcggtttc agaaagcagt ggggcatcga ccgattgacc
    63121 ggtaaactcc agacgcaaca tcggcacgca gtcagtgaat ggctccgctt gcagacgttc
    63181 ctggtaatct tccgggatat ccagatgcag ggtcgactga ataaacttct gcgccagcgg
    63241 cgttttcgga tgcgagaaca cttcacttac cgtgtcctgc tcgatcagtt cgccattgct
    63301 gatgaccgcc acgcaatcac aaatacgctt cacaacgtcc atttcgtggg tgatcaacag
    63361 aatcgtcaaa cccagacggc ggttgatgtc tttcagcagt tcgagaatag aacgtgtcgt
    63421 tgccggatcc agcgcgctgg tggcctcatc acacagcaat actttgggat tgctggctaa
    63481 cgcacgggca atcgccacac gctgtttctg cccaccggaa agattcgacg ggtagctatc
    63541 atgcttatcg ccaagaccaa ccaatgacag caattccgtc acgcgacgtt tgatctcatc
    63601 tttcggtgtg ttgtccagct ccagcggcag agccacgttg ccaaaaacag tacgcgaaga
    63661 gagcaggtta aaatgctgga aaatcatacc aatctggcgg cgagctttgg tcaactcgga
    63721 ttctgacagc gtggtcagtt cctggccatc gaccagcacg ctaccctcgg ttgggcgctc
    63781 cagcaggttt acacaacgta taagcgtact cttacccgcg ccagaggcac cgataacgcc
    63841 ataaatttgt ccagctggca catgcaggct gacgttgttc aacgcctgga tggtgcgggt
    63901 gccctggtgg aacactttgg tgatattcga aagttttatc attgattatt tattatcgtc
    63961 attaagttag tcgtggcatt tcgaatgcct ggaacgggca acgccttcaa tgaaatggat
    64021 gttaaggcat ccagacgtct aaatcaatca ggtttatgcg aagagcactt tcttgctggt
    64081 cgaaacatgc gatactagcg tcacatgcct tattaaggag ctataaaagg tggcgaagag
    64141 cgtacccgca atttttcttg accgtgatgg caccattaat gtcgatcacg gctatgtcca
    64201 tgagatcgac aactttgaat ttatcgacgg tgttattgac gccatgcgcg agctaaaaaa
    64261 aatgggcttt gcgctggtgg tagtaaccaa ccagtctggc attgctcgcg gtaaatttac
    64321 tgaagcacag tttgaaacgc tgaccgagtg gatggactgg tcgctggcgg accgcgatgt
    64381 cgatctggat ggtatctatt attgcccgca tcatccgcag ggtagtgttg aagagtttcg
    64441 ccaggtctgc gactgccgca aaccacatcc ggggatgctt ttgtcagcac gcgattattt
    64501 gcatattgat atggccgctt cttatatggt gggcgataaa ttagaagata tgcaggcagc
    64561 ggctgcggcg aatgtgggaa caaaagtgct ggtgcgtacg ggtaaaccta ttacccctga
    64621 agcagaaacg gcggcggatt gggtgttaaa tagcctggca gacctgccgc aagcgataaa
    64681 aaagcagcaa aaaccggcgt aatgattaaa agatgagcgg ttgaaataaa aaatgcattt
    64741 ttccgcttgt cttcctgaga cgactcccta taatgcgcct ccatcgacac ggcggatgtg
    64801 aatcacttca cacaaacagt
//
LOCUS       DAZFJL010000007        63872 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFJL010000007 DAZFJL010000000
VERSION     DAZFJL010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.007"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(25..738)
                     /gene="lpxT"
                     /locus_tag="ACHJ8C_000453"
     CDS             complement(25..738)
                     /gene="lpxT"
                     /locus_tag="ACHJ8C_000453"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO5550368.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPINHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENALGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            complement(777..1763)
                     /gene="yeiR"
                     /locus_tag="ACHJ8C_000454"
     CDS             complement(777..1763)
                     /gene="yeiR"
                     /locus_tag="ACHJ8C_000454"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO5550369.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALERWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHQHSVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(1881..3347)
                     /gene="yeiQ"
                     /locus_tag="ACHJ8C_000455"
     CDS             complement(1881..3347)
                     /gene="yeiQ"
                     /locus_tag="ACHJ8C_000455"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO5550370.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQNPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATNESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYPGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAVSSSEQRVTALLSLRE
                     IFGDDLPDNPHFVQAIEQAWQQIAQFGAHQALLNTLKI"
     gene            complement(3570..4142)
                     /gene="yeiP"
                     /locus_tag="ACHJ8C_000456"
     CDS             complement(3570..4142)
                     /gene="yeiP"
                     /locus_tag="ACHJ8C_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO5550371.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            4297..4551
                     /gene="yeiW"
                     /locus_tag="ACHJ8C_000457"
     CDS             4297..4551
                     /gene="yeiW"
                     /locus_tag="ACHJ8C_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO5550372.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(4548..5729)
                     /gene="setB"
                     /locus_tag="ACHJ8C_000458"
     CDS             complement(4548..5729)
                     /gene="setB"
                     /locus_tag="ACHJ8C_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO5550373.1"
                     /translation="MHNSPAVTSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     KVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            6097..7227
                     /gene="fruB"
                     /locus_tag="ACHJ8C_000459"
     CDS             6097..7227
                     /gene="fruB"
                     /locus_tag="ACHJ8C_000459"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO5550374.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            7227..8165
                     /gene="fruK"
                     /locus_tag="ACHJ8C_000460"
     CDS             7227..8165
                     /gene="fruK"
                     /locus_tag="ACHJ8C_000460"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO5550375.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            8182..9873
                     /gene="fruA"
                     /locus_tag="ACHJ8C_000461"
     CDS             8182..9873
                     /gene="fruA"
                     /locus_tag="ACHJ8C_000461"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO5550376.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            10329..11270
                     /gene="psuK"
                     /locus_tag="ACHJ8C_000462"
     CDS             10329..11270
                     /gene="psuK"
                     /locus_tag="ACHJ8C_000462"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO5550377.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVTSLVENTECLN"
     gene            11258..12196
                     /gene="psuG"
                     /locus_tag="ACHJ8C_000463"
     CDS             11258..12196
                     /gene="psuG"
                     /locus_tag="ACHJ8C_000463"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO5550378.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            12290..13540
                     /gene="psuT"
                     /locus_tag="ACHJ8C_000464"
     CDS             12290..13540
                     /gene="psuT"
                     /locus_tag="ACHJ8C_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO5550379.1"
                     /translation="MDIMRSVVGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANTSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(13607..14305)
                     /gene="yeiL"
                     /locus_tag="ACHJ8C_000465"
     CDS             complement(13607..14305)
                     /gene="yeiL"
                     /locus_tag="ACHJ8C_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO5550380.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYVTLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFINDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            14435..15376
                     /gene="rihB"
                     /locus_tag="ACHJ8C_000466"
     CDS             14435..15376
                     /gene="rihB"
                     /locus_tag="ACHJ8C_000466"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO5550381.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAENT
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            15476..16726
                     /gene="nupX"
                     /locus_tag="ACHJ8C_000467"
     CDS             15476..16726
                     /gene="nupX"
                     /locus_tag="ACHJ8C_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO5550382.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(16782..17870)
                     /locus_tag="ACHJ8C_000468"
     CDS             complement(16782..17870)
                     /locus_tag="ACHJ8C_000468"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO5550383.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(17874..18731)
                     /gene="nfo"
                     /locus_tag="ACHJ8C_000469"
     CDS             complement(17874..18731)
                     /gene="nfo"
                     /locus_tag="ACHJ8C_000469"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO5550384.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRIPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDSIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(18805..19854)
                     /gene="yeiH"
                     /locus_tag="ACHJ8C_000470"
     CDS             complement(18805..19854)
                     /gene="yeiH"
                     /locus_tag="ACHJ8C_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO5550385.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            19953..20834
                     /gene="yieE"
                     /locus_tag="ACHJ8C_000471"
     CDS             19953..20834
                     /gene="yieE"
                     /locus_tag="ACHJ8C_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO5550386.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            21039..22508
                     /gene="lysP"
                     /locus_tag="ACHJ8C_000472"
     CDS             21039..22508
                     /gene="lysP"
                     /locus_tag="ACHJ8C_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO5550387.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            22800..24791
                     /gene="cirA"
                     /locus_tag="ACHJ8C_000473"
     CDS             22800..24791
                     /gene="cirA"
                     /locus_tag="ACHJ8C_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO5550388.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTVQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(24823..25659)
                     /gene="yeiG"
                     /locus_tag="ACHJ8C_000474"
     CDS             complement(24823..25659)
                     /gene="yeiG"
                     /locus_tag="ACHJ8C_000474"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO5550389.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            25917..26585
                     /gene="folE"
                     /locus_tag="ACHJ8C_000475"
     CDS             25917..26585
                     /gene="folE"
                     /locus_tag="ACHJ8C_000475"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO5550390.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            26602..27759
                     /gene="yeiB"
                     /locus_tag="ACHJ8C_000476"
     CDS             26602..27759
                     /gene="yeiB"
                     /locus_tag="ACHJ8C_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO5550391.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKHWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWRLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANIFFSVIWLRYFRQGPVEWLWRQLTLRAAGPTISKTSR"
     gene            27901..28941
                     /gene="galS"
                     /locus_tag="ACHJ8C_000477"
     CDS             27901..28941
                     /gene="galS"
                     /locus_tag="ACHJ8C_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO5550392.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAADNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            29221..30219
                     /gene="mglB"
                     /locus_tag="ACHJ8C_000478"
     CDS             29221..30219
                     /gene="mglB"
                     /locus_tag="ACHJ8C_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO5550393.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            30280..31800
                     /gene="mglA"
                     /locus_tag="ACHJ8C_000479"
     CDS             30280..31800
                     /gene="mglA"
                     /locus_tag="ACHJ8C_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO5550394.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQSEILRLASLHL"
     gene            31816..32826
                     /gene="mglC"
                     /locus_tag="ACHJ8C_000480"
     CDS             31816..32826
                     /gene="mglC"
                     /locus_tag="ACHJ8C_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO5550395.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVVFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(33069..34304)
                     /gene="preA"
                     /locus_tag="ACHJ8C_000481"
     CDS             complement(33069..34304)
                     /gene="preA"
                     /locus_tag="ACHJ8C_000481"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO5550396.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANHNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(34298..35536)
                     /gene="preT"
                     /locus_tag="ACHJ8C_000482"
     CDS             complement(34298..35536)
                     /gene="preT"
                     /locus_tag="ACHJ8C_000482"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO5550397.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYLPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     IAADKIILAVGQHAELAAFSALEPQRNTIETQHYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAGAIHHYLEGACSC"
     gene            complement(35730..35969)
                     /gene="yeiS"
                     /locus_tag="ACHJ8C_000483"
     CDS             complement(35730..35969)
                     /gene="yeiS"
                     /locus_tag="ACHJ8C_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO5550398.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(35972..36691)
                     /gene="sanA"
                     /locus_tag="ACHJ8C_000484"
     CDS             complement(35972..36691)
                     /gene="sanA"
                     /locus_tag="ACHJ8C_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO5550399.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(36841..37725)
                     /gene="cdd"
                     /locus_tag="ACHJ8C_000485"
     CDS             complement(36841..37725)
                     /gene="cdd"
                     /locus_tag="ACHJ8C_000485"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO5550400.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            complement(37855..38550)
                     /gene="yohK"
                     /locus_tag="ACHJ8C_000486"
     CDS             complement(37855..38550)
                     /gene="yohK"
                     /locus_tag="ACHJ8C_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO5550401.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(38547..38945)
                     /gene="yohJ"
                     /locus_tag="ACHJ8C_000487"
     CDS             complement(38547..38945)
                     /gene="yohJ"
                     /locus_tag="ACHJ8C_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO5550402.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            39184..40131
                     /gene="dusC"
                     /locus_tag="ACHJ8C_000488"
     CDS             39184..40131
                     /gene="dusC"
                     /locus_tag="ACHJ8C_000488"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO5550403.1"
                     /translation="MRVLLAPMEGVLDSLVRELLIEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELHNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMTISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRMLNNSPDIARAIQAIDIEKL"
     gene            complement(40504..40587)
                     /gene="yohP"
                     /locus_tag="ACHJ8C_000489"
     CDS             complement(40504..40587)
                     /gene="yohP"
                     /locus_tag="ACHJ8C_000489"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO5550404.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            40811..42247
                     /gene="mdtQ"
                     /locus_tag="ACHJ8C_000490"
     CDS             40811..42247
                     /gene="mdtQ"
                     /locus_tag="ACHJ8C_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO5550405.1"
                     /translation="MNRDSFYPAIACFSLLLMLAGCAPMHETRQALSQQTPAAQVDTV
                     LPTALKNGWPDSQWWLGYHDNQLTSLINNALQSAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQLDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQSQIERDALRVVGLAQARFNVGLIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            42300..43061
                     /gene="yohF"
                     /locus_tag="ACHJ8C_000491"
     CDS             42300..43061
                     /gene="yohF"
                     /locus_tag="ACHJ8C_000491"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5550406.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     RKVVSHGVRAEIVQLDLGNLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMLKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDGGDVKPDAEPSIPLRRFGTTHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFKPE"
     gene            complement(43181..43759)
                     /gene="yohD"
                     /locus_tag="ACHJ8C_000492"
     CDS             complement(43181..43759)
                     /gene="yohD"
                     /locus_tag="ACHJ8C_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO5550407.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWSLIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            43929..44516
                     /gene="yohC"
                     /locus_tag="ACHJ8C_000493"
     CDS             43929..44516
                     /gene="yohC"
                     /locus_tag="ACHJ8C_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO5550408.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            44690..45622
                     /gene="pbpG"
                     /locus_tag="ACHJ8C_000494"
     CDS             44690..45622
                     /gene="pbpG"
                     /locus_tag="ACHJ8C_000494"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO5550409.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(45661..45768)
                     /locus_tag="ACHJ8C_000495"
                     /pseudo
     CDS             complement(45661..45768)
                     /locus_tag="ACHJ8C_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            45910..48207
                     /gene="bglX"
                     /locus_tag="ACHJ8C_000496"
     CDS             45910..48207
                     /gene="bglX"
                     /locus_tag="ACHJ8C_000496"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO5550410.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEEQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            48397..49314
                     /gene="osmF"
                     /locus_tag="ACHJ8C_000497"
     CDS             48397..49314
                     /gene="osmF"
                     /locus_tag="ACHJ8C_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO5550411.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDTAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLAELSRYLKEGGNF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQIAQWLQPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            49321..50478
                     /gene="yehY"
                     /locus_tag="ACHJ8C_000498"
     CDS             49321..50478
                     /gene="yehY"
                     /locus_tag="ACHJ8C_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO5550412.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTLWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIVPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAMLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGTQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            50471..51397
                     /gene="yehX"
                     /locus_tag="ACHJ8C_000499"
     CDS             50471..51397
                     /gene="yehX"
                     /locus_tag="ACHJ8C_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO5550413.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPDLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLQGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGEPCGTLHFQDLLEEA"
     gene            51402..52133
                     /gene="yehW"
                     /locus_tag="ACHJ8C_000500"
     CDS             51402..52133
                     /gene="yehW"
                     /locus_tag="ACHJ8C_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO5550414.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLKPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGANTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQAFSQHAK"
     gene            complement(52114..52221)
                     /gene="yohO"
                     /locus_tag="ACHJ8C_000501"
     CDS             complement(52114..52221)
                     /gene="yohO"
                     /locus_tag="ACHJ8C_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO5550415.1"
                     /translation="MRIDKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(52281..53012)
                     /gene="mlrA"
                     /locus_tag="ACHJ8C_000502"
     CDS             complement(52281..53012)
                     /gene="mlrA"
                     /locus_tag="ACHJ8C_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO5550416.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYISICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGD
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            53234..54919
                     /gene="btsS"
                     /locus_tag="ACHJ8C_000503"
     CDS             53234..54919
                     /gene="btsS"
                     /locus_tag="ACHJ8C_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO5550417.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            54916..55635
                     /gene="btsR"
                     /locus_tag="ACHJ8C_000504"
                     /gene_synonym="yehT"
     CDS             54916..55635
                     /gene="btsR"
                     /locus_tag="ACHJ8C_000504"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO5550418.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRLERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            55682..56152
                     /gene="yehS"
                     /locus_tag="ACHJ8C_000505"
     CDS             55682..56152
                     /gene="yehS"
                     /locus_tag="ACHJ8C_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO5550419.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(56193..56654)
                     /gene="yehR"
                     /locus_tag="ACHJ8C_000506"
     CDS             complement(56193..56654)
                     /gene="yehR"
                     /locus_tag="ACHJ8C_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO5550420.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(56779..58779)
                     /gene="yehQ"
                     /locus_tag="ACHJ8C_000507"
     CDS             complement(56779..58779)
                     /gene="yehQ"
                     /locus_tag="ACHJ8C_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO5550421.1"
                     /translation="MNSRRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQTLVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVALAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQALWLGGISQPPIHYEAAFSRAQQTAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGAGIAGEAKL
                     DHLRLVSLGMRCWQDIEQYGLRIWFTDPDTGSILHLSRSWPRSEQENAPAAKRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPINQVDNLFILPIAECISLGWDSSRQTLDAQVISGEGEDNVLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQKQG"
     gene            complement(58776..59912)
                     /gene="yehP"
                     /locus_tag="ACHJ8C_000508"
     CDS             complement(58776..59912)
                     /gene="yehP"
                     /locus_tag="ACHJ8C_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO5550422.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGDSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVHQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFISLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDHDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(59905..62184)
                     /gene="yehM"
                     /locus_tag="ACHJ8C_000509"
     CDS             complement(59905..62184)
                     /gene="yehM"
                     /locus_tag="ACHJ8C_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFO5550423.1"
                     /translation="MSEPLIVGIRHHSPACARLVKLLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDESPDTQDDSQTLLLRATCMDNSDTLWDHLFEDESQQTALPSTLAHYFA
                     QLRGDSPGDALNRQREAFMARWITWAMQQNNGDVLVVCGGWHAPALAKMWRECPQEIN
                     KPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQCGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVINPDTDPILVTLIDTLAGDGFGKLSPSTPQPPLPKDVTCELERTGISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGVVRQQGSTLSLAGNGEECWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGCITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHTHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGATLGAL
                     MRLKHPNASAETALTMLAQLSPAQSGEALHGLLALARHQLACQPVFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLVQLPVSALRMPLHCPPQDIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(62195..63283)
                     /gene="yehL"
                     /locus_tag="ACHJ8C_000510"
     CDS             complement(62195..63283)
                     /gene="yehL"
                     /locus_tag="ACHJ8C_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO5550424.1"
                     /translation="MSPQNNHLQRPPAAVLYAVELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDEAQNIRRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT        15482 a        15762 c        16996 g        15632 t
ORIGIN      
        1 cctgatgatg ttaattactg tgagttattt gttttggaaa tgtttgtttt ttccaggaag
       61 tgatttgtca aaaaaggtga ttaatcgatc acttaatggc gtcagcaaca cccagggcaa
      121 accgatcaat atcacggtca tggaaccgac gatgatgtca gtaaaccagt gtgcgccaat
      181 cattactctg ggaaacgcaa aaaccacaaa aataataagg gcgataaggc ctgcaacttt
      241 gccgaaataa cgccacatga atgccgaaaa aataagcagc atcataccgt gatcgccggg
      301 aaagctatct cgtgaggcat ctttcgtggg aacagagagc agttcgctga cgcggttaat
      361 attggtaaaa gtcaacgttg ggctggcccg ttttacagga attagtgcct gacccagctg
      421 gtttaatacc actgcagtta atagcatgac cagaccaata atcacgatac gtcgtctgcc
      481 tagggcgttt tctttcagcc agaaactcag catcaacatt cccatcgcca gcagtgaaca
      541 accgtcgaaa gcgcgattgt tggtcaatgc aaccagccat aaaaaggcct tactttcgac
      601 cagtttctga ttaaagaaat aaaaaatatc cgcatcaatc ggcaaccaga atccatgatt
      661 aatgggaata taccaggaaa gaaacagcgc gaggccgaca atattcaaca acactatttg
      721 cggcaaattt ttaatcatat tttctctaat tatcttactg aaaaacgcag gcaaccttac
      781 gcggtagtcg ctaaacgaag cttcaacagc gcgctctgca aggcattcca gtcagcttcg
      841 ctgctggaaa tcagctcaat acgactgtcc ggtggcgcaa cgttttgcgt ttcaatgtgc
      901 aggtcatcgc cctgacggtt gattcgcacc agcccttcgg gaatacgcag cacgcctttg
      961 acgcgttcca ccggtgcaag tcgcgcccat tccagaatac caatggtgtc gaataccgta
     1021 tcagcgtcga atatccagcc acaggcctga tgcccttgtc cgctgttcag actgcgacgc
     1081 cagcgttgat gctctggcag gcttaacgct gctaaccctt ttttcacgct atgctgatga
     1141 gaatgcgcgg cgctggcggg caactcggct aaattgcgac gcggcaaatc cagaagatga
     1201 ccgtcaattt ttccatgctc gctgtgaatt aattgccgat cgccaccatt ttgctgccac
     1261 cagcgctcta gcgcttgctc actttcgggc gtcgcacggt cggatttatt ggcgacaata
     1321 atgtctgcgg cagccagttg gtcacggaag ttttcattgc tggcgctttt ttcgtccagc
     1381 aacaggcgtg gatcgagaat gcacaacgtg gcgcgcagat ctatccacgg ttcatagacg
     1441 ggtgcggtta agagatcgag gatctgtttc ggatggccca gcccggtcgg ctctatcaac
     1501 aagcggtctg gttttccctg acgcagtaag gtattcaacc ctacctgcat gggtaaacca
     1561 ttaacgcagc acatgcagcc gccggggatc tctttcagca gtgcgccgct atcggcgagc
     1621 aaagcaccat caattccgac ttccccaaat tcattaacca gtaccgccca tttttcgttg
     1681 ggatctttat gggctaacag atgaagaatc gacgtagttt tcccgctgcc gagaaaaccg
     1741 gtgatgaggt tggttctggt cacaacggct ccgcaatact ctggtaagtt aactgagcgt
     1801 taatcctggc taaaaaaggc agaaaagaga aggggagagc ggacgaaggc gagcttcatc
     1861 cgctttaacc gcagaaatcg ttaaattttg agggtgttta atagcgcctg atgcgcgccg
     1921 aattgtgcga tttgttgcca ggcttgttcg atggcctgca caaaatgcgg gttatctggc
     1981 agatcatcac cgaaaatttc acgcagggaa agcagggcgg ttacgcgttg ttcactgctg
     2041 ctgaccgcaa caagttcacg aattttatcg ctaagcggat cgcgaacatc aatggcattt
     2101 ccggcatcat caacgccgct gacgtaacgc atccagcctg caacgcctaa tgccagcaac
     2161 gaccagtccg tttcgcgccc cagatgtatg cgaatacctg ccagcatgcg ttgcggtaat
     2221 ttttggctgc catccatcgc gatttgccag gtcttatgtt tcagtgccgg attagcaaaa
     2281 cgtgcaatta acttatccgc atattgtgtt aaatcgacat ctttaatccg cagtgtcggc
     2341 gcttgctcat ccagcattaa tgttctggcg gcatggcgaa atgcgcgatc ctgcatgcaa
     2401 tcactgatat gggcgaatcc tgagaggtaa cccggataag cgagaaaaga gtggctgcca
     2461 ttaagcatcc gcagtttcat ctcttcccat ggcaggacat cattcaccat ttgtacacct
     2521 gcgacttccc aggcaggacg cccagcgacg aagttatctt caaccaccca ctggataaac
     2581 ggttcgcagc taattgcgca gggatcattc acccccagat gctggcttat ttccgccagt
     2641 gattcgttgg ttgcagccgg aacaatgcgg tcgaccatag ttcccggaaa actgacgtgt
     2701 tcttttatcc acccggcgag ttctggcgaa cgtttttctg ccattcccag caccgcgttt
     2761 ttcaccacat ggccattgtc gggaatattg tcgcaggaga gcacggtaaa cggtgtaagg
     2821 ccgcgctcac ggcggcgttt cagtgcttcg acgagaatac ctggcgcgga gtgaggttct
     2881 tcagggtttt gtagatcgtg aataatacgc ggattgctgg tgtcgagtgc accggtggcc
     2941 gggtcaatac aatagccttt ttcggtaatc gtcagggaaa caattgccac ctgtggctcg
     3001 caaaatttct caataattgc cgctaaggaa tccagttttg cattcagaca ttcgtgaacg
     3061 gcaccgacaa ttatcgcctg attgccgtcc gcacctttct ccagcacggt atataaatgg
     3121 ttctgtgcgc ggagttggct catcagttga tcgccgctga acaagctgat ttcacagatc
     3181 ccccagtcgc cgccctgggc attcagcaca cgatcggtca gtaatgcctg atgtgcgcgg
     3241 tgaaaggctc caaagccaaa atgaacgata cgtgattgca actgctgtcg atcgtatcgt
     3301 ggagcgtgta catgatgcgg gagcgtaacg gaggcgattg tattcatagg agtcacgtct
     3361 tagtaacgat taattaacaa tgacagtgta aagttatatg acaacaattg aaattggtct
     3421 ggattgttaa tgcgatgaag tgtgagggcg atcaatcaac gagtgaaaat tgcgggcgta
     3481 aggtgttcag gaagcgcaaa atagaagatt ggtataataa attaaaaaag ggacagcctg
     3541 agctatccct tttctgcatg cggctgaagt tagtcagcac gacccatata acggcgttct
     3601 tcgatatgga tacgaatttt ttcgcccggg cttaagtatt ctggtacctg aatcaccaga
     3661 ccagtgctca atgtcgccgg tttgttacgg gcgctggcag atgccccttt gatgcctggt
     3721 gcagtttcaa cgatttccag atcaacggtc tgcggcagct caagcgccag caattgaccg
     3781 tcccaggtca gcacctgcat gtccggcatg ccgccctcag gcataaacag caactcttct
     3841 tcaatctgat ctttggtgaa ggtatacggg gtatagtctt ctttatccat aaagacatat
     3901 tcgttgccat cgacatagga gaagtcaacg taacggcggg tcagcgtcac ggtgtcaacg
     3961 atatcatcac ctttgaaacg ctcttctact ttcagcccgg tacggacatc agaaaaacgc
     4021 attttgtaca gcgtagcggc accgcgggca gtgggcgact gaatatcaat gtccttaacc
     4081 agcagcagtt tgccgttgta attcagtacc ataccttttt taatttcgtt cgctcttggc
     4141 atcgaaaaat tcctgtagtc aaaaatgtca aaaatatcgc gacaaagtac tcgcgacggg
     4201 cctttcgggc aagtggtatt cgcacttttg ctggtgcaaa aaaggtggta ctgtgcgcgc
     4261 tcatcaatcc ggtggttaac cttaagagaa caacgtatgg aatgccgtcc gggttgcggg
     4321 gcgtgttgca ctgccccttc aatttccagc cctattcccg gtatgccaga tggcaagccc
     4381 gccaatacgc cctgcattca gcttgatgaa cagcaacgct gtaaaatttt cacctcgcca
     4441 ctgcgaccga aagtctgtgc aggcttacaa gccagcgctg aaatgtgcgg taactcgcgt
     4501 cagcaggcaa tgacctggct tatcgatctg gaaatgttga ccgcgcctta aacatcttta
     4561 attcgcagta agcaaaacag agtggcgata atcatcacca tcgcaaacca gaacacagcg
     4621 tgataattcc agatctcggc gacgatgccc gccactgatc ctgcgatgat ccagcccacg
     4681 cgcgacgtgt tggtatagag cgtggtggct gaacctgcct gaccgggcat caaatcctga
     4741 aaatagagca tcccgatgcc gcccagaatg ccaataaaaa tagcatttag cagctgcaag
     4801 cccaacagaa tgacaggtga atgcgccatc agcattcctg cgtaaaaaca gacgccaccc
     4861 acggcagcaa cgcgcattaa gaagcgctta cccagacgtt tggcaaaata tccggcgatc
     4921 aacatggtcg ggatttccag cccggcggcg gtccccatca tcacaccggc cagtttctcg
     4981 ggaagatgca gttcgttgat aataaatagc ggcatgttga tgatgtacag gctgttcgag
     5041 ccccacatca atgtacaaat gacaaacagc agcaacgtat cgcgacggtt acggcgcggc
     5101 gcttcaaccg tgccggtcgc cagcggaagc tctttttgca tcgacggtaa aaacagccac
     5161 accatcacgc cgcaaacaat aaacgctacc gctgcgctca gatacattac cgtaaagctg
     5221 aaacccatcg ctaaggcata agccagcggt gggccaatga cccatgccag tgaaacctga
     5281 gcgcgtaaaa aagagctgaa catcaccgcc tcacgtccgg ttttgtcagc atgttcacgg
     5341 gcaagggcaa acatttgcgg gttagcggtt gagccaaagc tgctaagaaa gacgccaacg
     5401 aatagcaaaa caaagtagtt gcgattccag gcaaaaaggg tgcaggccag cacgcctaac
     5461 aggcagcaaa agacaatcag cgatttgcga tcgccgcgct tatcagagcg cccggcgaga
     5521 aactgactca ccagaatccc aatgactgcg ctgccggtaa agaagaatcc caccatcgcc
     5581 ggacgggcat gtactttatc agtaagaaaa atactgagtg tcggggtttg cagagcgccc
     5641 gcaataccgg tgagaaaggc aacgattaaa aacgccgtcg aggtcaggtc aaacgatttc
     5701 gcgctggtga ctgcggggga gttatgcatg ctggtcgatc actggaagag gaagacgcgg
     5761 agtttacgct gcttgtcaga aaataaacag acagcgaaat caaaagcgcc acgaataatc
     5821 aaaatgccat ttcactgttg ctgattggct gctgacgctg ctgaaatgaa attgctgata
     5881 acggattttc ccatcagcaa ttaggaaaaa tgacaaaaaa ttgtgcagca catcaaactt
     5941 ttgctcataa ctttacggct ttccttgcgt gctgaaacaa aagtattatg ctttcttgaa
     6001 acgtttcagc gcgatcttgt ctttaaccct aagccaggtt ggcgcttttt ttctcataga
     6061 agctgaatcg tttcaattca gcaagagagg agaactatgt tccagttatc cgtacaggac
     6121 atccatccgg gcgaaaaggc cggagacaaa gaagaggcga ttcgccaggt cgctgcggcg
     6181 ctggtgcagg ccggtaatgt agctgaaggc tacgtcaatg gcatgctggc gcgcgaacag
     6241 caaacctcaa cgttcctcgg caatggtatt gctattccac acggcactac cgacacccgc
     6301 gatcaggtgc tgaaaaccgg cgttcaggta tttcagttcc cggaaggcgt cacctggggt
     6361 gacggccagg tagcgtacgt ggcgatcggt attgctgcca gctcggatga acatctgggc
     6421 ctgctacgcc agctgaccca cgtactgagc gatgattccg ttgctgaaca actgaagtca
     6481 gcaacaacag cagaagaact tcgcgcattg ctgatgggcg aaaagcagag tgagcagctg
     6541 aagctcgaca acgaaatgct gacgctggat atcgtcgcca gcgatctgct gactcttcag
     6601 gcgctgaacg ctgcgcgtct gaaagaggcg ggggcagttg acgccacttt cgtcaccaaa
     6661 gccatcaatg aacaaccgct gaacctcggg cagggtatct ggctgagcga tagcgccgaa
     6721 ggcaatctgc gtagcgcgat tgcggtaagc cgtgcggcaa atgcttttga tgtggacggc
     6781 gaaacggcag ccatgctggt gagtgtggcg atgaatgacg atcagcccat cgcggttctt
     6841 aagcgtctcg ctgatttgtt gctcgataat aaagctgacc gcttgctgaa agcggatgcg
     6901 gcaacgttgc tggcgctgct gaccagcgat gatgcgccga ccgacgacgt gttaagcgcg
     6961 gagtttgtgg tgcgcaatga acacggcctg catgctcgtc caggtaccat gctggtcaat
     7021 accattaaac aatttaacag tgatattacc gtgacaaacc ttgatggcac cggcaaaccg
     7081 gcaaacggac gtagtctgat gaaagttgtg gcacttggcg ttaagaaagg tcatcgccta
     7141 cgctttaccg cccagggtgc agatgctgaa caggcgctga aagccattgg cgacgctatc
     7201 gctgctggtc ttggggaggg cgcataatga gcagacgtgt tgctacgatc acccttaatc
     7261 cggcttatga ccttgttggt ttctgcccgg aaattgaacg cggcgaagtg aacctggtga
     7321 aaaccaccgg tctgcatgcg gcgggtaaag gcatcaacgt ggccaaagta ttaaaagacc
     7381 tgggaattga tgtcaccgtt ggcggcttcc tgggtaaaga caatcaggat ggttttcagc
     7441 aactgttcag cgagctgggc attgccaacc gtttccaggt tgtacagggg cgcacccgaa
     7501 ttaacgttaa gctgacggaa aaagacggcg aagtgaccga cttcaacttc tcgggttttg
     7561 aagtcacccc cgccgactgg gaacgctttg tgactgattc tctgagctgg ctcggtcagt
     7621 tcgatatggt ctgtgtcagc ggaagcttac cgtcaggcgt cagcccggaa gcgttcaccg
     7681 actggatgac tcgcctgcgt agtcagtgtc cttgcattat ctttgatagt agccgtgaag
     7741 cgttagtagc aggtttgaaa gcggcaccgt ggctggtgaa acctaaccgc cgcgagctgg
     7801 aaatctgggc aggccgtaaa ctgcctgaaa tgaaagatgt gattgaagct gcgcatgcgc
     7861 tgcgtgaaca aggcatcgcg catgttgtta tttcactggg tgccgaaggc gcgctttggg
     7921 ttaatgcctc cggcgaatgg atcgccaaac caccgtcagt cgatgtcgta agcaccgttg
     7981 gcgcagggga ttctatggtt ggtggcctga tttatggctt gctgatgcgt gaatccagtg
     8041 aacacacact gcgtctggcg acagctgttg cagccctggc ggtaagtcaa agcaatgtgg
     8101 gtattaccga tcgtccgcag ttggccgcaa tgatggcgcg cgtcgactta caacctttta
     8161 actgacagca ggagaggcat aatgaaaacg ctgctgatta ttgacgctaa tctcggtcag
     8221 gcacgcgcct atatggcgaa gaccctgctg ggcgcggcgg cgcgaaaagc aaaactggaa
     8281 atcatcgaca atccgaacga cgcagaaatg gcgattgttc tcggtgattc catcccgaat
     8341 gacagcgcgc tgaacggtaa aaatgtctgg ctgggtgata tttcccgggc agttgcgcac
     8401 cctgagttgt tcctgagtga agccaaaggc catgcgaaac cttacactgc gccggtcgct
     8461 gcgacagcac cggttgctgc cagcggtccg aaacgcgtag ttgcggtgac tgcttgcccg
     8521 actggcgtag cacacacctt tatggcggct gaagccattg aaaccgaagc gaaaaaacgt
     8581 ggctggtggg tgaaagttga aacccgtggt tctgttggcg cgggtaatgc aatcacccct
     8641 gaagaagtcg cagcagcgga tctggtgatt gtggcggcag atatcgaagt ggatctggcg
     8701 aaatttgctg gtaaaccgat gtatcgcacc tctaccggtc tggcgctgaa gaaaactgcg
     8761 caggaactgg ataaagcggt tgctgaagca acgccgtatg aaccagcggg caaagctcaa
     8821 acagcgacca ctgaaggtaa gaaagagagt gcaggcgctt accgtcactt gctgacgggc
     8881 gtctcttaca tgctgccgat ggttgttgca ggtggtctgt gtatcgcgct ttcttttgct
     8941 tttggtatcg aagcgtttaa agagccgggt acgttggcag cggcgctgat gcagattggt
     9001 ggtggttcag cctttgcgct gatggtgccg gtactggcag gttatattgc cttctccatt
     9061 gccgatcgtc cgggtctcac gccgggtctg attggcggta tgctggcggt tagcaccggt
     9121 tctggcttca ttggcggtat tattgcgggc ttcctggcgg gttacattgc gaagttaatc
     9181 agtacgcaac tgaaactgcc acagagtatg gaggcactga aaccgatcct gatcattccg
     9241 ctaatttcca gtctggtggt cggtctggcg atgatctacc taatcggtaa accggttgct
     9301 ggcattctcg atgggttgac tcactggctg cagaccatgg ggactgcgaa tgcggttctg
     9361 ctgggggcga tcctcggtgg catgatgtgt actgacatgg gcggtccggt aaacaaagca
     9421 gcgtacgcat tcggtgtggg tctgctgagt actcaaacct atggcccgat ggcggcgatt
     9481 atggcggcag gtatggtgcc accgctggca atgggtctgg caacaatggt ggcgcgtcgc
     9541 aaattcgaca aagcgcagca ggaaggtggc aaagccgctc tggtacttgg tctgtgcttt
     9601 atttcggaag gtgcaattcc gtttgctgct cgtgatccga tgcgtgtgct accgtgctgt
     9661 atcgttggtg gggcgctgac tggcgcaatc tcaatggcga ttggtgcgaa actgatggca
     9721 ccacacggtg gtctgtttgt tctgctgatc cctggcgcta ttacgccggt attgggttac
     9781 ctggtagcaa ttattgccgg tacgctggtg gcgggtttgg cctatgcctt cctgaaacgc
     9841 ccggaaacac aaatagtaga aaaaaacgca taatttatct tcattatcgg ggatgagaca
     9901 tctctgcccc gatgatgaca aacgcaaaat tgcctgatgc gctatgctta tcagacctac
     9961 atgaaccctg caatatattg aatttttgcg cttttgtagg tcggataagg cgtttacgcc
    10021 acatccagca tgaacaaagc gcgcgttgtc aacaatctga gggcagaaac atctctgccc
    10081 tttttcttgc ctgccatatt gtgtgttaca catcacaact ttctcttttg ttgctgcata
    10141 aatagacaaa ctttgtgacc gccttcatat ctggccgatt tcgttttttc tgatcgattt
    10201 acattgcgtt aaacatgctt attatgaaca tgtgtttaaa tctgaacacg tgctctaata
    10261 gtcttattga ctgtaatgac tattttaccc aatttcttcg ttggcaattt attgccaggg
    10321 ggcgtgctat gcgcgaaaag gactacatag taattattgg ctcggcgaat attgatgtgg
    10381 ctgggtattc acatcatcca ttgaattatg cggattcgaa tcccggaaaa attaaattta
    10441 ccccaggtgg cgtgggaagg aatatcgctc ataatctggc gttactgggg aaaaatgcct
    10501 ggcttttaag tgcagtaggt ggtgattttt atgggcaatc attactggcg caaactaatc
    10561 aatccggtgt ttatgtcgat aaatgcttaa ttgttccggg tgagaataca tccagctatt
    10621 tatcactgct cgataataca ggagaaatgc ttgtcgcgat aaatgatatg agtattagtg
    10681 actgtatttc tgcagaattt cttgcccagc atcaggagtt tattcgcggt gcaaaggtta
    10741 ttgtcgcgga ttgcaacctt agtgaagagg cgctggtctg ggttctggaa aatagcgggg
    10801 aaacgcctgt atttattgat ccggtatcgg catggaaatg cgtcaaaatc cgcgatcatc
    10861 tgagcaaaat ccatacgtta aaacctaacc gtcttgaagc cgaaacgttg agcggaattg
    10921 cactttccgg acgtgaagat gtcgcaaagg tcgccgcctg gtttcatcaa cacggcctga
    10981 accgcctggt gctgagcatg ggaggggatg gcgtttacta cagtgatatc aacggtgaaa
    11041 gtgggtggtc ggcccctatt aaaacgaatg tcatcaatgt tactggggca ggcgacgcca
    11101 tgatggcggg gctggcctcc tgttgggtag acggtatgcc gtttatcgat tctgttcgat
    11161 ttgcgcaggg atgttcgtca atggcacttg cctgtgaata taccaacaac cctgaattat
    11221 caattgccaa tgttacatcg ttagtggaga acacagaatg tctgaattaa ctctgtcccc
    11281 tgaattatta caaatatccg ctgaagtaca ggatgcttta aaaaataaaa aaccggttgt
    11341 tgcgctggaa tcgaccataa tttctcacgg aatgccattc ccacaaaatg cccaaaccgc
    11401 aattgaagta gaagaaacaa ttcgtaaaca gggcgcagta ccagccacta tcgccattat
    11461 tggcggtgtg atgaaagtgg gcttaagtaa agaagaaatc gaattgctgg gccgtgaagg
    11521 acataacgtg acgaaagtta gtcgtcgtga tttacctttt gtcgttgctg cagggaaaaa
    11581 tggcgcgact accgttgcat caaccatgat tattgcagca ctggctggga ttaaagtttt
    11641 tgcaaccgga ggtattggtg gtgtgcatcg cggagcggaa cataccttcg atatttctgc
    11701 cgatttacag gaactggcaa ataccaatgt cactgttgtt tgtgcggggg caaaatctat
    11761 tctcgattta ggattaacaa cagaatattt agaaaccttc ggtgtaccgt taattggtta
    11821 tcagacaaaa gcgctgcctg cgtttttctg ccgaaccagc ccatttgaag tcagcattcg
    11881 tctcgacagt gcccgcgaaa ttgctcgcgc gatggcagtg aagtggcaaa ccggtttgaa
    11941 cggcggcctg gtggtagcga acccgatccc ggaacagttt gcgatgccag aagaaaccat
    12001 caatgcagca attgatcaag ccgttgctga agccgaagaa cagggtgtta ttgggaaaga
    12061 gagtacaccg ttcctgctgg cgcgtgttgc ggagctaacc ggcggtgata gcctgaaatc
    12121 caatattcaa ctggtcttta ataacgccat tctggcgagc gaaattgcca aagaatatca
    12181 gcgtctcgtg ggttaatttc tggtgggcag aaagccgtcg cccgtactca tcaaacatac
    12241 caaaacctgg cgctgacgcc gggtttactg gcatgaaggg aaaatcatta tggatataat
    12301 gagaagtgtt gtggggatgg tggtattgtt ggcaattgcc tttgtgttgt cggtaaataa
    12361 aaagagcatc agcttgcgca cggttggggc tgcgctgctg ctgcaaattg ccattggtgg
    12421 catcatgctc tattttcccc cgggaaaatg ggcagtagag caggcggcat taggcgtcca
    12481 taaagtgatg tcctacagtg acgcaggaag tgcctttatt tttggttcgc tggtcggtcc
    12541 caaaatggat gttctgtttg acggtgcggg ctttatcttc gcctttcgcg tactcccggc
    12601 gattattttc gttaccgcgc ttatcagctt gctgtactac attggcgtaa tggggctgct
    12661 gattcgtatt cttggcagta tttttcagaa agccctcaac atcagcaaaa tcgaatcttt
    12721 tgtcgcagtc accactattt tcctcgggca aaatgagatc ccggcgatcg tgaaaccttt
    12781 tattgatcgc atgaatcgca acgagctatt tacggctatt tgtagcggca tggcgtctat
    12841 tgcgggttcg atgatgattg gctatgccgg aatgggcgtg ccgattgact atttgttagc
    12901 cgcttcgctg atggctattc ctggcggtat cctgtttgca cgtattctta gtccggcgac
    12961 ggaaccttcg caagttacgt ttgaaaatct gtcgtttagc gaaacaccgc caaaaagcat
    13021 tattgaagct gcggcgaacg gtgcaatgac cgggctgaaa attgcagccg gtgtggctac
    13081 ggtggtgatg gcgtttgtcg caattattgc gctgatcaac ggcattatcg gcggaattgg
    13141 cggctggttt ggttttgcca atacctctct ggaaagtatt tttggctatg tgttggcacc
    13201 actggcgtgg atcatgggtg tggactggag tgatgccaac cttgcgggta gcctgattgg
    13261 gcagaaactg gcgattaatg agtttgttgc ttatctcaat ttctcgccct atctgcaaac
    13321 gagcggcacg ctggatgtca agaccattgc cattatctcg ttcgccctct gtgggtttgc
    13381 aaactttggt tctatcggtg ttgtcgttgg agcgttttcg gctatttcgc caaaacgcgc
    13441 gccggaaatc gcccagcttg gtttacgggc gctggcggcg gcgacgcttt ccaacctgat
    13501 gagcgcgacc attgccgggt tctttattgg tttagcttga gtttcattgc cggatgtacg
    13561 ccttattaag cgttgcatcc ggcaatcgca ctattgccaa taatttttac tgcatcatcc
    13621 cggagaattt attctccggg tccatctcca gcgccagtcc tgacaactgc tttctgtttt
    13681 taatgagata ccctttcttg cttttaatta ataaaccgtc attaatgaat tgcgcaagaa
    13741 catataaaag atgtcgataa gaaacgccta aatactctgc cgcttgcgta tgcttttcgt
    13801 gataaagatc accttcctgc gagagtaata taaaagctgc caggcgattc actaacggaa
    13861 atgattgatt ctgagttaaa gaaacaatat tacgataatt tttatgactt aaggtgacgc
    13921 agagttttcg taaaaatagc gtgtcgttta ataacaacgg gcggtaatgc ttcataggga
    13981 gcgcaagaca ccaacactct tcaatagcct gcactgcacg cggttcatgg tctttgtcga
    14041 ttaactcaat ctcgccaata aaacagggcg cggcaaagaa atcgatcagc gacacgcgac
    14101 cattagcaag cgtgacgtaa agcctggcgc ggcctcgcgt caggtaaaac agccaggacg
    14161 gctgttgccc ttcctgcaca atgtagtcac gcgctaaaaa atgaaacagc cgcgtatcgg
    14221 ctgaaacatc cgttgaaaag caatccttaa acgcagattc actcatcaat tgctgcttaa
    14281 gatcattatt gtggatttct ttcacgatgt ttatccgcta tgagatttct catatttagc
    14341 atacgtacgc tgttgttaag attcaatcat cggccaggtc gaattttctt tgctgtagaa
    14401 acaataacgc aactggaaac agaggaaata aacaatggaa aagagaaaaa ttattctgga
    14461 ttgtgatccg ggtcatgatg atgctattgc tataatgatg gcggcgaaac atccggcgat
    14521 tgatttatta ggtatcacca ttgtagcggg taatcaaacc ctcgataaaa cattaattaa
    14581 tggcctgaat gtttgccaga aactggagat taatgttccg gtttatgcgg ggatgccgca
    14641 gcccattatg cgtcaacaaa ttgttgccga taatattcac ggtgaaaccg gactggatgg
    14701 cccggtattc gagccgctga cccgccaggc agaaaacact catgcggtga aatatatcat
    14761 cgataccctt atggcaagcg atggcgatat cactctggtg ccggttggtc cgctttcaaa
    14821 tatcgcggtg gcaatgcgta tgcagcccgc gatcctgccg aaaatccgtg aaattgtact
    14881 gatgggcggc gcttacggta caggcaactt cacgccatct gccgagttca acatctttgc
    14941 cgacccggaa gccgcacgcg tggtgttcac ctccggcgtt ccattagtga tgatgggcct
    15001 cgatctcacc aaccagaccg tttgcacccc ggacgtgatt gctcggatgg aaagggcagg
    15061 cggccccgcc ggagagctgt tcagcgacat catgaacttc actctgaaaa cgcagttcga
    15121 aaactacggt ctggctggcg gcccggtaca tgacgccacc tgtatcggtt atctgattaa
    15181 ccccgatggc attaaaaccc aggagatgta cgtcgaagtg gacgtcaaca gtggcccttg
    15241 ttacgggcgt accgtctgcg acgagctggg cgttcttggc aagcccgcca atacaaaagt
    15301 cggcatcact attgataccg actggttctg gggattagtc gaagagtgcg tgcgcggcta
    15361 catcaaaacc cactaagtgg tgatatcaag ccgggccggt atcgctggat gcaagcctgg
    15421 ctatttctga tgggcagaaa gccgtcgccc aaaatgacat gaagagataa taactatgga
    15481 tgtcatgaga agtgttctgg gaatggtggt attgctggca attgcatttt tgctgtcagt
    15541 aaacaagaag aagatcagcc tgcgtaccgt tggcgcggca ttagtgttac aggtcgtgat
    15601 tggcggcatt atgctttggt taccgccagg gcgttgggtc gcagaaaaag tcgcttttgg
    15661 cgtccataaa gtgatggcgt acagcgacgc gggtagcgca tttattttcg gttctctggt
    15721 cggaccgaaa atggatacct tatttgatgg cgcaggattt atctttggtt tcagggtgtt
    15781 accggcaatt atcttcgtca ccgcgctggt gagtattctc tactacatcg gtgtgatggg
    15841 gatcttaatt cgaattctcg gcggtatctt ccagaaagca ttaaatatca gcaagatcga
    15901 gtcattcgtc gcggtcacta ccattttcct cgggcaaaac gaaattccgg caatcgtcaa
    15961 accctttatc gatcggctga atcgcaatga attatttaca gcgatttgca gcggtatggc
    16021 ctcgattgct ggttcgacaa tgattggtta cgccgcactg ggcgtgcctg tggaatatct
    16081 gctggcggca tcgttaatgg cgatccccgg cgggatcttg tttgcccgcc tgttaagccc
    16141 ggctacggaa tcttcgcagg tttcctttaa taacctctct ttcaccgaaa caccgccaaa
    16201 gagcattatt gaagccgctg caacaggggc aatgaccggg ctgaaaatcg ccgcaggtgt
    16261 ggcgacagtg gtgatggcat ttgttgcaat aattgcgttg attaacggta ttatcggcgg
    16321 tgttggcggc tggtttggtt ttgaacatgc ttcgctggag tcgattttag gttacctgtt
    16381 ggccccattg gcgtgggtga tgggggttga ctggagtgat gcaaatcttg ccgggagttt
    16441 gattgggcag aagctggcga tcaatgaatt tgtcgcttat ctcaatttct cgccatatct
    16501 gcaaacgggt ggcactctgg atgctaaaac cgtggcgatt atttctttcg cgttgtgcgg
    16561 tttcgctaac tttggttcta tcggggtggt ggtgggggcg ttttctgcgg ttgcgccaca
    16621 ccgtgcgccg gaaatcgccc aacttggttt acgcgcgctg gcggcggcga cactttctaa
    16681 cctgatgagt gctactattg caggattctt tattggttta gcgtagtgcg caatttatga
    16741 gccggataag atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat tggcactaat
    16801 gaaagcgcgt tatcggcaga cagggtaggg ttgtttaagc tgccgctggc gcgtgaaatt
    16861 gccgcgcagg ccatcgcaaa acgggcgctg tcgcggaaac tgtatccttc aagaaagcta
    16921 tataccaggc ccgccataaa accatcgtca gcaccaaaac tgtctactgt cgtatgcgct
    16981 ggcgcggtca gcaaaaattg ttctccatcc ttatcgctgc aaaatacaga ttcatctttc
    17041 agataaacaa aaatgcgttt aacaccttgt tgatgcagcg aatttacagc tctgatgcga
    17101 tcgttgtcgt ctttaatcgg ttgcccccac aaaatttcca gttcattttg tgttggtttc
    17161 agagtgtgga tccggctgta ccagtttttt actttgttcg ctttaaattc ggaaacggta
    17221 tcgacaaaca tcggaatttc atcagcaatg gtaaagaccc actccagggc ttcaggtgtg
    17281 agattacaat cagccagcac cacaccagca tggcgaatca agtcccgtga agtattgagc
    17341 agttgaggcg ttagctgttg cagaatatgt gtgtcattga ttgccaggat agtttcttcc
    17401 tgcttattag cgatagcgag ataagttgca gtgctgtgtc catgcagacg aatgcagttt
    17461 gaaacgttca cgcccgcgcg gcgcgtttct tccagtaatg tctcgccgta aaaatcgttg
    17521 ccaatagctg atattaaatg cacatcccga ccaagcagag ccaggttatg cgcgatatta
    17581 cgtccgacgc cgcctgcgga acagtgaaca gaacctggat gagaggctgc ttgcgggtaa
    17641 cggatatccg ccattccgcg aatatccata ttgattgcac ccacaaccac acagtaatcc
    17701 tgctcggtaa ggatatatcc tttgccttta atcaaaccct tgcgcatcaa atccataata
    17761 tgagcggcaa cacgtgagcg gctgatttgc agaatatcgg cgatttcatt ctgttgaatc
    17821 agcgggttac gccgtaaaat tttaagaatc tgcttttccc tgtcgttcat cgatcaggcc
    17881 accgcttttt cagtttgttg cgctttcagc caagcgatct cttctgccca gatatccggg
    17941 ttgatggttt caaggatcag cggaatgctg tcgaaacggt cgtcttgcat gatccagcgg
    18001 aacgcatcat gaccgatatt gccttcgccg aggctatgat gacggtcaac gcggctgcca
    18061 aaggtgcttt tcgcatcgtt gaggtgcatc ccgcgcagat acttaaagcc aacaatacgg
    18121 gcaaaatcag cgaacgtttt ctcgcattcg gcaggaatac gcaaatcata cccggcagcg
    18181 aaagcatggc aggtatcaat gcagacgccg acgcgggatt tatcttccac gccgtcgata
    18241 atcgccgcga gatgttcgaa tttaaaccct aagttactgc cctgaccggc ggtgttttca
    18301 atcaccgcgg tgacaccttg agttttatcc agcgcaatgt tgatggattc ggcaatacgc
    18361 gcaaggcaat cctcttctga aatctgcatc agatggctgc cagggtggaa gttgagcaaa
    18421 gaaagcccca gctgttcgca acgctgcatt tcatctataa aggcatcgcg cgatttttcc
    18481 agagcttcag caaccggatg tccgaggtta atcagataac tgtcgtgggg aagaatttgc
    18541 gccgatgtgt agtgatattt ttcacaggcg gctttgaatt catcgatggt ttgcgtcgtg
    18601 agcggtgcgg cacgccactg acgttggttt ttggtgaaca aggcaaacgc ggttgcgtcg
    18661 atttcggcgg cgcgaattgc ggcatttgcc agaccgccag cagcactaac gtgcgctcca
    18721 atgtatttca tgcgaggact cctgttaaac ccgctggagg aaaacggtaa tgatagcggg
    18781 ttaacaggag caagatgtag tggtttatgc gatgacgctt tgaatcacat agttaatcgc
    18841 accaccacca acaatcagcc aggcaaacag taccagtgcc atcagcagag gtttcgcccc
    18901 ggcttttttc agcgcgctga cgtgagtggt cagacccagc gccgccatcg ccattgccag
    18961 caggaaggta tccagcgtta ccagcatgtt caccacgctc tgcggtaaca ggtggaacga
    19021 gttaaagatg gcaacgacga tgaacaagat ggcaaaccac ggaatagtga ttttgctttt
    19081 ctcgccgctg ttcgccccag acagctgttt aacacgcgcc gccagcagga tgaggaacgg
    19141 agccagcatc atcacgcgca gcattttgga aataactgct gcgttttctg catccgggct
    19201 gatggcatga cctgccgcca ccacctgcgc cacttcgtgc acagtagaac caatgtagat
    19261 accgaaagtt tccggactaa accattgaga catcagcgga tatatcgccg ggtagaggaa
    19321 aatcgcgacg gtcccgaaga taacaacggt tgcaacagcc acggttactt tactggcttc
    19381 tgctttcact accggctcag tcgccagtac cgcggcagca ccacagatac tgctaccggc
    19441 accgatcaac cagctggtgt gcttatccag accaaacact ttctgcccca ggaagcaagc
    19501 cagcaggaag gtactggaca gcgtcaacac gtcaatgatg atcccactga taccgacatc
    19561 ggcaatttgc gagaacgtca gacggaagcc ataaagaatg atacccagac gtaataaata
    19621 ttgcttggca aacagcacac caccgtcaca gcttttccag atgtgcgggt aaatggtgtt
    19681 gcctaaaacc atccccaaca agattgcgag ggtgagggca ctaaacccgg cacccgcaac
    19741 cgcgggaatg gaaccacccc acagggcgac cccggtgata actgcactca gggctaaccc
    19801 cggaataaaa tgccacagtg tacgatgttg tttctgtaag gtgatattcg tcataaccct
    19861 ctcctttaac cgggcataag gttacgacta actggtttaa aaataaaatt gattatatat
    19921 ttataattaa tctttataag tggtaagcga ctatgcacat caccctccgg cagttggaag
    19981 tttttgcaga agtattgaaa agtggatcaa ccacccaggc gtcggtgatg ctggcgttgt
    20041 cgcaatcagc agtgagcgca gccttgaccg acctggaagg gcagcttggc gttcaactgt
    20101 ttgatcgcgt ggggaaaaga ctggttgtta atgaacacgg gcggctgctc tatccgcgag
    20161 cgttggcact gcttgaacag gcggttgaaa tcgaacaact gtttcgcgaa gacaacggcg
    20221 cgattcgtat ctatgccagt agtaccatcg gtaactacat tctgcctgca gttatcgccc
    20281 gttatcgcca tgattatccg cagttgccga ttgaacttag cgttgggaat agccaggacg
    20341 tgatgcaagc ggtgctggat ttccgcgttg atattggctt tattgaagga ccgtgccaca
    20401 gcactgaaat catttctgaa ccgtggctgg aagacgagct ggtggttttc gccgcgccga
    20461 cttcgccgtt ggctcgtggt ccggtcacct tagaacagct ggccgctgcg ccgtggatcc
    20521 tgcgtgaacg cggttccggc acgcgggaga ttgtcgatta tctgttgctg tcacatttac
    20581 cgaagtttga gatggcgatg gaattaggta actccgaggc aatcaaacat gcggtgcgtc
    20641 atgggttggg aattagttgt ctgtcgcgac gggtgattga agatcaattg caggcaggca
    20701 cattaagtga agttgcggtc cctctgccgc gcctgatgcg tacgttgtgg cgtatacatc
    20761 atcggcaaaa acacctttcc aacgcgctac ggcgctttct ggactattgc gatcccgcga
    20821 atgtaccgcg ttaaggtgct gtacaagaac atgctggtgc tgtgtcgatt tcgtgacgca
    20881 gcgccctcag catgcattcg ccagaaaaga gattggctgc tttacttata atccctgggc
    20941 gatcatgaag gtgtcttata accgtgtatt tctgccggaa ggattgccaa tcgtctgcta
    21001 caatcgcgcc tcatttttaa gatggatagc atttttgtat gggttccgaa actaaaacta
    21061 cagaagcgcc gggcttacgc cgtgaattga aggcgcgtca cctgacgatg attgccattg
    21121 gcggttccat cggtacaggt ctttttgttg cctctggcgc aacgatttct caggcaggtc
    21181 cgggcggggc attgctctcg tatatgctga ttggcctgat ggtttacttc ctgatgacca
    21241 gtctcggtga actggctgca tatatgccgg tttccggttc gtttgccact tacggtcaga
    21301 actatgttga agaaggcttt ggcttcgcgc tgggctggaa ctactggtac aactgggcgg
    21361 tgactattgc cgttgacctg gttgcagcac agctggtcat gagctggtgg ttcccggata
    21421 caccgggctg gatctggagt gcgttgttcc tcggcgttat cttcctgttg aactacatct
    21481 ctgttcgcgg ctttggtgaa gcggaatact ggttctcact gatcaaagtc acgacagtta
    21541 ttgtctttat catcgttggc gtgctgatga ttatcggtat cttcaaaggc gcgcagcctg
    21601 caggctggag caactggaca atcggcgaag cgccgtttgc tggtggtttt gcggcgatga
    21661 tcggcgtagc tatgattgtc ggcttctctt tccagggaac cgagctgatc ggtattgctg
    21721 caggcgagtc cgaagatccg gcgaaaaaca ttccacgcgc ggtacgtcag gtattctggc
    21781 gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag cctgattatt ccgtataccg
    21841 atccgagcct gctgcgtaac gatgttaaag acatcagtgt cagtccgttc accctggtgt
    21901 tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa cgcagttatt ttgacggcgg
    21961 tgctgtcagc aggtaactcc ggtatgtatg cgtctactcg tatgctgtat accctggcgt
    22021 gtgacggtaa agcgccgcgc atttttgcta aactgtcgcg tggtggcgtg ccgcgtaatg
    22081 cgctgtatgc gacgacggtg attgccggtt tgtgcttcct gacctccatg tttggcaacc
    22141 agacggtata cctgtggctg ctgaacacct ccgggatgac gggttttatc gcctggctgg
    22201 ggattgccat tagccactat cgtttccgtc gcgggtacgt attgcaggga cacgacatta
    22261 acgatctgcc gtaccgttcc ggtttcttcc cactggggcc gatcttcgca ttcattctgt
    22321 gtctgattat cactttgggc cagaactacg aagcgttcct gaaagatacc attgactggg
    22381 gcggcgtagc ggcaacgtat attggtatcc cgctgttcct gattatttgg ttcggctaca
    22441 agctgattaa aggaactcac ttcgtacgct acagcgaaat gaagttcccg cagaacgata
    22501 agaaataagt ttcctccctt ccttgctaag ccctctcaac cgagagggct ttttcaattc
    22561 catttccctg acaaatcatg cggatataaa atttaacatt tggattgata attgttatcg
    22621 tttgcattat cgttacgccg caatcaaaaa aggctgacaa atcagaggct gttccggctt
    22681 tctgggatga tcacctgcat acaaataagt ccaccgcgat gctgccgtac gcaaggggac
    22741 gtaaagaaga tgtgagcgat acccatttta ttttcgtagt tacctcatgg agatatggaa
    22801 tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt gtccgctatt tcttgtgcat
    22861 ggcctgtgtt agcggtcgat gatgatggcg aaacgatggt tgtcactgca tcttccgttg
    22921 aacaaaacct taaagatgct cccgccagta tcagcgtcat tacccaggaa gacctgcagc
    22981 gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt gcctggcgta caactgacga
    23041 acgaagggga taaccgtaag ggcgtaagta ttcgtggtct ggacagcagc tacaccctga
    23101 ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt cttccgccac aatgatttcg
    23161 atctgaactg gatcccggtc gattccatcg aacgtattga agtggtccgt ggcccgatgt
    23221 cgtcgctgta cggttccgat gcgctcggcg gtgtagtgaa tatcatcacc aaaaaaatcg
    23281 gtcagaaatg gtcgggcacc gttaccgtcg ataccaccgt tcaggaacat cgcgatcgcg
    23341 gtgataccta taacggtcaa ttctttacca gcggaccatt aattgacggc gtgctgggaa
    23401 tgaaagctta cggcagcctg gcaaaacgtg aaaaggatga cccgcaaaac tcaacgacca
    23461 ccgataccgg agaaacgccg cgtattgaag gattctccag ccgcgacggc aatgtcgaat
    23521 ttgcctggac accgaatcaa aatcacgatt ttactgccgg atacggtttc gaccgtcagg
    23581 atcgtgattc cgactcgctg gacaaaaacc gcctggaacg ccagaactac tccgtcagcc
    23641 ataatgggcg ttgggattac ggcaccagcg aactgaaata ctacggtgag aaagtcgaga
    23701 acaaaaaccc tggcaacagc agcccgataa cttccgaaag caatacggtc gacggcaaat
    23761 acacgttgcc gctgacggcg attaatcagt ttctcacggt tggcggtgaa tggcgtcacg
    23821 acaaacttag cgatgcggtg aacctgaccg ggggaaccag ctccaaaacg tctgccagcc
    23881 agtacgcgct gtttgtggaa gatgaatggc ggatcttcga gccgctggcg ctgacgaccg
    23941 gcgtgcgtat ggacgatcac gaaacctacg gtgaacactg gagtccgcgt gcctacctgg
    24001 tttataacgc caccgacacc gtaacggtga aagggggctg ggcgacggca tttaaagccc
    24061 cttctctgtt gcaacttagc cctgactgga cgagcaattc ctgccgtggc gcatgtaaga
    24121 ttgtgggtag cccggatctg aaaccagaaa ccagcgaaag ttgggagctg gggctttact
    24181 acatgggtga agaaggctgg ctggaagggg ttgaatccag cgttaccgtt ttccgtaacg
    24241 atgtgaaaga tcgtatcagc attagccgta cgtctgacgt caatgctgca ccaggctacc
    24301 aaaactttgt cggttttgag acgggcgcta acggacggcg cataccggta tttagctact
    24361 acaacgttaa caaagctcgt attcagggcg tggaaaccga actgaaaatt ccgttcaacg
    24421 atgaatggaa actgtcgatc aactacacct acaacgatgg tcgtgatgtc agcaacggcg
    24481 aaaacaaacc gctatccgat ctgccgttcc atactgctaa cggtacgctg gactggaaac
    24541 cgctggcgct ggaagactgg tcattctatg tttctggtca ctataccggg cagaaacgcg
    24601 ccgacagcgc gacggctaaa acaccgggcg gttataccat ctggaatacc ggcgcggcct
    24661 ggcaggtgac taaagacgtc aaactgcgcg caggcgtgct gaaccttggc gacaaggatc
    24721 tcagtcgtga cgactacagc tataacgaag acggacgtcg ttactttatg gcagtggatt
    24781 atcgcttctg atgagaagat gcccggcgaa ccgggcggac tttcacttca gtaaatactg
    24841 cgcatggaag cgcaggtgat cctctataaa agaggcgatg aagtagtaac tgtgatcata
    24901 tcccggctga atacgcagcg tcatcggcca cgctttctgg cgcgcggctt ctgccagtac
    24961 tgcaggttgc agttggtcag caagaaactg atcattatca ccctgatcaa taagcgtcgg
    25021 gattgcatcc tgcgcgttac tggcatacat cagcgcgcaa ctgtcccatt ccagccatgc
    25081 atttttgtcc tcacctaaat aagtgctaaa cgctttgatt ccccacggga cgctgcacgg
    25141 attcacaatt ggcgcaaagg cggaaacgct ggtgtattta cccggatttt tcagcgccat
    25201 aatcagcgca ccgtgaccac ccatggaatg accgctaata gcgcagcggt cgctgacatt
    25261 aaattgcgac tgaaccagcg ccggtaattc atcgcgcaga taatcataca tccggtaatg
    25321 cgtcgcccac ggtggttgcg tggcgttaag ataaaacccc gcgccctggc ctaaatcgta
    25381 gccatcgtcg ttggcaacct gttcgccgcg cgggctggtg tctggcatca ccagtacaat
    25441 ccccagttcc gccgccacgc gctgggcacc cgccttggtg gtgaagttct cgtcattgca
    25501 ggttaatccg gaaagccagt acagcactgg tggcggggtg tgatcacgag gtggtgggag
    25561 aaagatactg aacgtcatcg ggcagtttaa ggtactggag tcgtgtcgcc agcgttgctg
    25621 ccagccttca aaacagcggt gctcttcgag catttccatg cgtggctcct tgttgtgttg
    25681 tttgcaattt cttcatcata ccgtttattc aatgtgctgt gagtaacttt cacttccgta
    25741 tttgcataac gatgttttaa catctgctga tgaaaggcag cggcaattac aataattatc
    25801 gctgtgaata ctggattatg tgcgccgcct cacgcacaat aatcagactg taaatcagct
    25861 taataacttt gcccccacgc agggcggagg cgtcacacct gcaggagaaa tcataaatgc
    25921 catcactcag taaagaagcg gccctggttc atgaagcgtt agttgcgcga ggactggaaa
    25981 caccgctgcg cccgcccgtg catgaaatgg ataacgaaac gcgcaaaagc cttattgctg
    26041 gtcatatgac cgaaatcatg cagctgctga atctcgacct ggctgatgac agtttgatgg
    26101 aaacgccgca tcgcatcgct aaaatgtatg tcgatgaaat tttctccggt ctggattacg
    26161 ccaatttccc gaaaatcacc ctcattgaaa acaaaatgaa ggtcgatgaa atggtcaccg
    26221 tgcgcgatat caccctgacc agcacctgtg aacaccattt tgttaccatc gatggcaaag
    26281 cgacggtggc ttatatcccg aaagattcgg tcatcggtct gtcaaaaatt aaccgtattg
    26341 tgcagttctt tgcccagcgt ccgcaggtgc aggaacgtct gacgcagcaa attctcattg
    26401 cgctacaaac gctgctgggc accaataacg tggctgtctc tatcgacgcg gtgcattact
    26461 gcgtgaaggc gcgtggcatc cgcgatgcaa ccagtgccac gacaacgacc tctcttggtg
    26521 gattgttcaa atccagtcag aatacgcgcc acgagtttct gcgcgctgtg cgtcatcaca
    26581 actgataaaa aggcaggaac cgtggagcgc aacgtcacgc tcgattttgt tcgcggcgtc
    26641 gccattctgg ggatcctgct attaaacatc agcgcctttg ggttaccaaa ggcggcttat
    26701 ctcaatcccg cctggtacgg cgctattacg ccacaggatg catggacctg ggcatttctc
    26761 gatctcatcg gccaggtgaa attcctcacg ctttttgcgc tgttgtttgg tgcgggcctg
    26821 caaatgttgc tgccccgtgg cagacgatgg atccagtcgc ggttaacgct gttagtcttg
    26881 ctgggcttta ttcacggttt attgttctgg gacggcgata ttctgctggc ttacgggctg
    26941 gtgggcttaa tctgctggcg gctggtgcgc gatgcgccat cggtaaaaag cctgtttaat
    27001 accggcgtca tgctttatct ggtggggctt ggcgttttgc tgttattggg gttgatttcc
    27061 gatagccaga ccagccgcgc ctggacacct gatgcatcgg ctattttgta tgaaaagcac
    27121 tggaaacttc acggtggtgt tgaagcgatc agtaatcgtg ccgatggtgt tggcaacagt
    27181 ttactggcac tgggcgcaca gtatggctgg caactggcag ggatgatgct cattggtgcc
    27241 gcattgatgc gcagtggctg gctgaaaggg cagttcagct tacgtcacta tcgtcgtact
    27301 ggttttgtgc tggtggcaat tggggtgatc attaaccttc ctgccatcgc cctgcaatgg
    27361 cgactggact gggcgtatcg ttggtgcgcc ttcttacttc agatgccgcg ggaactgagt
    27421 gcgccgtttc aggcgattgg ctatgcgtcg ctgttttatg gcttctggcc gcaattaagc
    27481 cgctttaagc tggtgctggc aatcgcctgt gtcggacgga tggcactgac caactatcta
    27541 ttgcaaacgc tgatttgtac tacgcttttt taccacctcg gtttgtttat gcagtttgac
    27601 cgtctggagc tgctggcgtt tgttattccg gtatggttgg cgaatatctt cttctctgtt
    27661 atctggctgc gttacttccg ccaggggccg gtggaatggc tctggcgtca gttaactttg
    27721 cgtgctgccg gaccgacaat atctaaaaca tcaagataac gatctggatc acattcgtta
    27781 acaaaacggc tgtaaccgtt tccattgctg tgactcgatt cacgaagtcc tgtattcagt
    27841 gctgacaaaa tagccgccag caagcagtca tttactgcaa tctcataaca ggtagtgaat
    27901 atgatcacca ttcgtgatgt agcgcgtcag gctggcgtct ctgtggcaac ggtttcccgg
    27961 gtgctcaata acagcacgct ggtcagtgcc gacacgcgtg aagcagtaat gaaagctgtg
    28021 agtgagctgg attatcggcc aaacgccaat gcccaggcgc tggcaactca ggttagcgac
    28081 accattggcg tggtggtgat ggacgtttct gatgcttttt tcggcgcgct ggtaaaagcg
    28141 gtggatctgg tcgcccagca gcatcagaaa tacgtgctaa tcggcaatag ctatcatgaa
    28201 gcggaaaaag agcgtcacgc cattgaggtg ttaattcgcc agcgttgtaa tgcgttgatt
    28261 gttcactcaa aagcattgag tgacgatgaa ctggcacaat ttatggataa cattcccggt
    28321 atggtgttaa tcaaccgcgt tgtgccgggg tacgcccatc gttgcgtttg cctggataat
    28381 ctcagcggtg cccgaatggc gacgcgcatg ttgctgaata acggtcatca acgtattggt
    28441 tatctttctt ccagccacgg cattgaagat gacgccatgc gtaaagcagg ctggatgagt
    28501 gcgttgaaag agcaggatat tattccgccg gaaagctgga ttggcactgg tacgccggac
    28561 atgccgggcg gtgaggcggc gatggttgaa ctgctggggc gcaatctaca actaaccgct
    28621 gtatttgctt ataacgacaa tatggccgct ggcgcactga cagcattaaa agataatggc
    28681 attgcgattc cgttacatct ctcaatcatt ggtttcgatg atattcccat cgcccgttac
    28741 accgacccgc aattaacgac tgtgcgttat cccattgctt caatggctaa attagctacc
    28801 gaactggcct tgcagggggc agcagacaat attgatcctc gtgccagcca ctgttttatg
    28861 ccgacgttag tgcgtcgcca ttctgtcgca acacgccaga atgcggcggc gatcactaac
    28921 tcaacaaatc aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca
    28981 atgtgatggc tatgattgca ccgttttaac gttgtaacca gtatgtaaca gtgaataatc
    29041 actttcaccg aggtaatagc gtcataacaa caattaaagc cgttttctgg agcgttaccg
    29101 ggcatggaag aacgaatttt aaaaagtgag cttcggcgtt cagtaacact tcattaactc
    29161 tactgccccg ccgagcattt atctcaagca ctaccctgca taagaaaaac cggagatacc
    29221 atgaataaga aggtgttaac cctgtctgct gtgatggcca gcatgttatt cggtgccgct
    29281 gcacacgctg ctgatactcg cattggtgta acaatctata agtacgacga taactttatg
    29341 tctgtagtgc gcaaggctat tgagcaagat gcgaaagccg cgccagatgt tcagctgctg
    29401 atgaatgatt ctcagaatga ccagtccaag cagaacgatc agatcgacgt attgctggcg
    29461 aaaggggtga aggcactggc aatcaacctg gttgacccgg cagctgcggg tacggtgatt
    29521 gagaaagcgc gtgggcaaaa cgtgccggtg gttttcttca acaaagaacc gtctcgtaag
    29581 gcgctggata gctacgacaa agcctactac gttggcactg actccaaaga gtccggcatt
    29641 attcagggcg atttgattgc taaacactgg gcggcgaatc agggttggga tctgaacaaa
    29701 gacggtcaga ttcaattcgt actgctgaaa ggtgagccgg ggcatccgga tgcagaagca
    29761 cgtaccactt acgtgattaa agaattgaac gacaaaggca ttaaaactga acagttacag
    29821 ttagataccg ctatgtggga taccgctcag gcgaaagata agatggacgc ctggctgtct
    29881 ggcccgaacg ccaacaaaat cgaggtggtt atcgccaaca acgacgcgat ggcaatgggc
    29941 gcggtagaag cactgaaagc acacaacaag tccagcattc cggtgtttgg cgtcgatgcg
    30001 ctgccagaag cgctggcgct ggtgaaatcc ggtgcactgg cgggcaccgt actgaacgat
    30061 gctaacaacc aggcgaaagc gacctttgat ctggcgaaaa acctggccga tggcaaaggt
    30121 gcggctgatg gcaccaactg gaaaatcgac aacaaagtgg tccgcgtacc ttatgttggc
    30181 gtagataaag acaacctggc tgaattcagc aagaaataag actgattggg cgcagtcgtt
    30241 tactgcgccc ttattgtatg gccaacaagg taaaattata tggtcagctc aacgactccg
    30301 tcttccgggg aatacttgtt ggaaatgagc ggtatcaaca agtcttttcc tggtgttaag
    30361 gcacttgata acgttaattt aaaagtccga ccacattcta tccatgcatt aatgggggaa
    30421 aacggtgcag gaaaatcgac attattaaaa tgcctgtttg gtatttatca aaaagactcc
    30481 ggcaccattt tattccaggg taaagagatc gatttccatt ccgccaaaga agcactggaa
    30541 aatggtattt cgatggtaca ccaggagtta aacctggtat tacaacgttc ggtgatggac
    30601 aacatgtggc tggggcgata tcccaccaaa ggcatgtttg tcgatcagga caaaatgtac
    30661 cgcgaaacca aagcgatttt tgatgaactg gatattgata tcgatccgcg tgcgcgcgtc
    30721 ggtacattat ctgtttcgca aatgcagatg atcgaaattg ccaaagcgtt ttcctataac
    30781 gcgaaaattg tgattatgga tgaaccgact tcttcgttaa ccgaaaaaga ggtcaatcat
    30841 ctgttcacta ttattcgtaa attaaaagag cgcggctgcg gtattgttta tatctcgcat
    30901 aaaatggaag aaatcttcca gttatgtgat gaagttaccg tattgcgcga cggtcagtgg
    30961 atcgctaccg aaccgctggc agggctgacg atggacaaga tcatcgccat gatggttggg
    31021 cgttctctta accagcgttt ccctgacaaa gaaaacaagc cgggcgaagt gattttggaa
    31081 gtgcgtaacc tgacgtcgct gcgccagccg tcgattcgcg atgtctcgtt tgatctgcat
    31141 aaaggggaga ttctcggtat agccggtctg gtgggagcga aacgtaccga tattgttgag
    31201 acgttatttg gtattcgcga gaaatcggct ggcaccatta cgttgcacgg caaaaagatc
    31261 aataaccata atgccaacga agccataaac cacggatttg cactggtaac tgaagagcgc
    31321 cgctcaacgg gaatttatgc ctatctggat attggcttta actcgttaat ttccaatatc
    31381 cgcaactata aaaataaagt tggtttactg gataactcgc ggatgaaaag cgatacccag
    31441 tgggtgattg attcgatgcg ggtaaaaaca ccggggcatc ggacgcaaat tggttcgctc
    31501 tccggtggta atcagcaaaa agtgattatt ggtcgctggc tgttaacgca accagaaata
    31561 ttaatgctcg atgaaccgac gcgcggtatt gatgttgggg ctaagtttga gatttatcag
    31621 ttaattgccg aactggcgaa gaaaggcaag gggattatta ttatctcctc cgaaatgcca
    31681 gagttgttag ggataacaga ccgtattctg gttatgagca atggtctcgt ttccggaatt
    31741 gtcgatacaa aaacaacaac gcaaagcgaa attctgcgtc ttgcgtcttt gcacctttaa
    31801 gattaggggc ttcccatgag tgcgttaaat aagaaaagtt ttcttactta cctgaaagag
    31861 ggcggtattt acgtcgttct tttagttttg ctggcgatta ttattttcca ggacccaaca
    31921 tttttaagtc tgttgaactt aagtaatatt ctcacccagt catcggtgcg tattattatc
    31981 gcgcttggtg tggcagggtt aattgtcacc caggggactg acctttccgc aggtcgccag
    32041 gtggggctgg cggcagtagt agcagcgacg ttattgcagt ccatggataa cgccaacaaa
    32101 gtcttcccgg aaatggcgac gatgccgatt gcgctggtta ttctgattgt ctgtgccatt
    32161 ggtgcggtga tcggtttgat caacggcctg attatcgcct atctcaacgt gacgccgttc
    32221 attaccacgc tcggtacgat gattatcgtc tacggcatta actcgctcta ttacgacttt
    32281 gtcggggcgt cgccaatttc cggttttgac agtggtttct ctacctttgc tcaaggcttt
    32341 gtcgcgctgg ggagtttccg tctctcttac atcaccttct atgcgttgat tgcggtggtg
    32401 tttgtctggg tgttatggaa caaaacccgc ttcggtaaga acatttttgc cattggcggt
    32461 aacccggaag cggcgaaagt atctggtgtc aacgtcggcc tgaacctgct gatgatctac
    32521 gcgttgtctg gtgtgttcta tgccttcggc gggatgttag aagccggacg tatcggctca
    32581 gccaccaaca acctcggctt tatgtatgag ctggatgcta tcgcggcgtg cgtggtaggc
    32641 ggcgtatcgt tcagcggcgg tgtggggacg gtaattggtg tggtgaccgg ggtaattatt
    32701 tttaccgtca ttaactatgg cctgacgtat atcggcgtaa acccgtactg gcagtacatc
    32761 atcaaagggg cgattattat cttcgccgtg gcgctggatt cactgaaata cgcgcgtaag
    32821 aagtgatgtg attgtggtcc gcattcgttc ataaacgcac ataacatcat gatctgtgtg
    32881 gttttgtagt ccggataagg cgttcacgcc gcatccggcg acaggaacca aatgccggat
    32941 gcaacgctgg cgcgttttat ccggcctaca atgagcacaa catactgatt tatattgttt
    33001 tgtatgccgg ataaggcgtt caagccgcat ccggcgacag gaactaaatg ccggatgtga
    33061 cgcgggtttt acaacgttac cgggtgttct ttctcgcctt tcttaaactt cacttcccca
    33121 agatcaatac aacctaccgg gcagacatga ccacaaagca gacaacccac gcatttctcg
    33181 gtattacaat gcggtgtgcg ggttttctcg ctccattcca tcgcctggtg accgccgtcg
    33241 taacaggaaa tataacagcg tccacagcca acacatttat caagattgat atggggatag
    33301 acaatataac tgcggtctaa atcttccgcc gggacaatat tgtgattcgc cagacccacc
    33361 atctcctgca acgaatcaaa cccttgatcg gcgagataat gcgacaaccc gcttgccata
    33421 tcttccacta tccgataccc gtactgcata atgccggtgg tcacctgcaa cgttgctgcg
    33481 ccgagcaata aaaactccgc cgcatcttcc caggtttcaa tgccgccgat accgctgatc
    33541 gggaaatcgc gcagttctgg atgggtgcgc atttgctgaa taaagcgtag ggcgatcggt
    33601 tttaccgctt taccggaata tccggaaata ctcgatttac cgttaacgat cggcatacca
    33661 attttctgat taagatcgat attggtgatg gatttaacgg tgttaatcgc cgcaatgcca
    33721 tctgcgccgc cgcgcttcgc cgccagcgcc acttcgcaca tatcaccaat attcggcgtc
    33781 atcttcgcca gcattggcag cgtggaacca cgttttactg cccgacaata tttttctacc
    33841 agctccgggc tttgcccgac atcgctaccc atcgcatgag aagtcatttg cggacaggag
    33901 aagttacact cgatcatatc cgcgccagct tcttgcacca gacgcgccag ctcttcccat
    33961 tgctgctcat tttcgcccat gattgaagcg atcaatactt tgtccgggta atcttctttc
    34021 agccgacgca gggcggccag attctcttcc aacggatgtt cagcaatctg ctccatattt
    34081 ttgaagccga taaaaccggt atcttctttc accagatgat caaaacgcgg cgagacttcg
    34141 ttggcgataa aaaaaccgat cgttttaaac accacgccgc cccaacctgt gtcgtaggct
    34201 ttggcgcaca tctcatagca gttgcctacc ggcgaagaag aaaggcagaa cgggttggga
    34261 aacttcacgc cgcaaaaagt aatcgaaaga tctttcgtta acatgagcaa gctccctcta
    34321 aatagtgatg aatcgccccg gcggcttctt tcccggtttt cactgcatag accacggttt
    34381 tgtcaccctc aacaatatcg ccagcagcaa agacttgcgg gtcgcgggtc tggtaatgtt
    34441 gcgtttcgat ggtgttacgt tgcggttcca gtgcggaaaa agcagcaagt tcggcatgtt
    34501 gacctacagc gagaataatt ttgtccgccg ctatcgttag ttcacccggt aaacgaacat
    34561 gcttaaaggt gactttattg ccttccacgg ctactggtgt aaatccatcg atgatcgaaa
    34621 cgcccagctc tctggcgctg gtaaactctt tttcgcttgc cggaaactca tctaactctt
    34681 cacgcgctac gcaggttacc gcctgacagc cgagaacttt cagcgtgctg gctacgtcca
    34741 tcgcgacatc accgccgccg ataattaatg cgctttgtgg aatgctgata tcgccttgcg
    34801 cctgtcgtgc acgttgcagg aagtcgacgg caatctcaac gtcgctatgc tcaaacagcg
    34861 ataacccgga accgctcgat aaccctacgg tgaccagtac cgcgcggttt tccgctttca
    34921 gctgctccag ggtgagtgtt ttaccgattt cgttattgca cttaatggtc acgcccattt
    34981 tttctatacg ggcgatttct gcatccagca ctgattgcgg taaccggaat tgcggaatgc
    35041 cgttacgcaa ccagccaccg ggttgtgctt ctttctcata gatcgtgacg tcataaccct
    35101 ggtttgtcag cgtcacactg gcctgcaatc cggcaggacc tgcgccaata atggcgactt
    35161 tgccgagcgt tttagtaccg ggcagataaa tttccatccc agtttgttgt tcaaaatcag
    35221 taacaaaacg ctgtaagcga ccgatatcaa tgggagcatc aacaccggca cgtgtacaac
    35281 cgctttggca taatttctcc gtcgggcaca ctctggcaca aacggcaccg agggcattat
    35341 tttcgcgaat tgtctcggca gcgcctttaa aattacgaaa gtagattgag cgaataaatt
    35401 tccccggatc ggtctgtgcc gggcaagcct gactacaggg agcgtcatga cataataaac
    35461 agcgagaggc ttctttaatc gccagtaaag gcgtaaaagc cggggtgagt tcatccagat
    35521 aattttgttg cggcatgtga tcttccttaa tgctatgagt aattaaggcg aacgctaaat
    35581 aaatattgcg tgctgatatt aaggctatgc gcgtggggaa atttaaactg tgaaaggatt
    35641 cacaaatagc tgcggttcag aatgtgatat ccgcagctta ttgagtgaag taataattat
    35701 tctcaataat taacagaaaa gggaaaatat taacgataga aaataagata aacaatcatg
    35761 ccgatagata aaattccgaa gccgatatat gccaccatat aagcgaagaa gagtccggga
    35821 tttttcgctc gccagacata caccggttct ggtgcatttt taacccgttt atcaatcgcg
    35881 cctgcgcgcc agcctttcca tgcttcgcgg aaacaaaata tcgggataag gaaaaaaacg
    35941 gcaacgacaa aaaactgctg tacatccata actactttcc ttgtttcttt tgtaattcaa
    36001 gtaactgttc gggtgtgacg gcgggatacc cctgcgcatc ttccggcacc tggtgcatag
    36061 ccggaatagg gaccagcggc cctaaaaaac gcggttcacg tttaaaaata taaaggtcag
    36121 ccagcgcacc gaaacgggcg gcaaattcgc gaatacgtac tgacagcata tctttcggtg
    36181 acggtacggc ataacactga gcctgaatcc ccatatgcag cgcaataaat aatgcccgct
    36241 cacagtggaa acgttgggtg ataataatga aatcattagt atcgaaaact ttgcgtgtac
    36301 gcacgatgga atccagcgta cgaaagcctg cgtaatcgag aacaatatct gatgggtcga
    36361 caccagctgc gattaaatct ttgcgcatgg tcatcggctc attataactt tgcaatgcgt
    36421 tatcgccgct cagtaataga taatttacct taccgctgtt ataggcatta atcgctcctt
    36481 gaatgcggta gcgataatac tgattaatta cgccagtccg ataatatttt gctgtgccga
    36541 gcaccacacc gacctggcgg taggggagat cctgcaattc gtcgtagata taaggcgcgg
    36601 ttttccagct catccagcga tcgaggccga gcacagtcaa cagcagcaag ccgatcagga
    36661 ctaacaggct gaggaacacg cgctttaaca tgcaggagac tcgatctaag gaaaatttcc
    36721 atcaggctac tttacccgct ggttttgcgc aagaaacggc aaactgtttg ccgggatttt
    36781 cgacaccagg aaagggctgg caagccggac aaggcgttca cgccgcatcc ggcaccaggc
    36841 ttaagcgaga agcacgcggt cgatattgtg acagccgaga gctttcagcg ttgcggaggt
    36901 ggcatcccac tgaatcaacg gcgcatcggc tttttctgcc agaaccgcgc gctggatatc
    36961 cgggtaatca taacccttga gattcaacag aattaacgcc ccctgcaacg gcggcagagt
    37021 cgggttgaat gcggcgtttt cagcgtagct gccgctgaaa atacgaccgt ctttacattc
    37081 cagcgcgaca ccgcttggcg acttactgta aggcatgtgc gaacggtttg ccgccgcaat
    37141 cgccgcctga gaaagcgcat cacccgtcag cgcatagccg tgatcctgct cgtccatcag
    37201 cagcgtttta atctccaaat ctttcggccc aaaggcatcc ggtagatagt cacgcagcgc
    37261 gtgtgcctcg cgtcccggaa gatgaatacg cagatccaga ccgctgttca gttcattcat
    37321 aaactgacgg cagtgaccac aaggcgtgta gttaacggtg atggctgcaa gcgctttttc
    37381 accactcagc caggcgtggc tgatcgcgct ttgttcggca tgaacggttt gctgcattgt
    37441 cgcaccaata aattccatat tggcaccgaa ataccaggtt ccgctcacac cgcgcgcaat
    37501 tgcgccaaca ttaaaattcg acaatggcgt acgcgcacag gctgccgcca gcggaagtag
    37561 tgcgaatgcc agcgcgtctt cgtccagccc cgttgcgctc ttcagcgatg agacttgctc
    37621 cccggtcaac aaagcgggga agtacttgtc tgccagaata ggttccagtg cagattgcaa
    37681 gttatccgca agttgggcaa aagcggtttg aaaacgtgga tgcatggcgt tgcctcataa
    37741 tcatgtaatg ggataacagt ttacgaaccc gttatggctt tatatgtgat ctgaatctca
    37801 ttaatcatgc aacgcgtgaa acttacatca aaaatgcgcg acgcatcgca aattttagcc
    37861 cattactgcc agaataatcg ggaaaaggaa cggtgcgatc agcgaagtaa taatcccgca
    37921 taacaccagc gccagcgaac taaatgcacc ttcctgataa tccagctcgg cgcagcgcgc
    37981 cgtaccgagg gcgtgcgagg ctgtccccat tgccagaccg cgcgcagctt tggtacgaat
    38041 acgcatcgca ttaagcaatg tatggccaaa cacagcgccg aggatgccga cgaaaatcac
    38101 gcaaactgcg ctgattgccg gaataccgcc aatactgccg ccaaccgcca ttgcaatagg
    38161 cgtggtgact gatttcggca ggattgacgc ggcgatttcc ggtgaagcgc ccatcaacaa
    38221 tgccacggaa gtccccgtca ccatcgcaac cacgctgccg ataaaacaga tggtgatgat
    38281 cgatttccag cgcgcgcgga tctggtgtag ctgctcatat aaaggataag ccagtgcgac
    38341 gaccgccggt tgcagcagat cgtttagcac ttcgctacct ttaaagtagc tgtcgtaaga
    38401 gatgccagtc agcattaaaa aaggaatgat caccaccatt gctaccagca acgggttaag
    38461 caggggaaac ttataccgtg ccgccagttt gcgggcggca aaaaagacaa tcaatgtcag
    38521 cggtaatgac caccagatat tcgccatcat tcttctgatc ctttctgacc tacgacttta
    38581 cgttcaccgt gcaccagttg cgaactccag ctcaccacca gaaaaaccac cagcgtactg
    38641 actgcacagg aaaccactac cggaccaaac tgtgcgcgga gcaaatcaaa atattgcatg
    38701 acgcctacgc caatcggcac aaacaatagc gccatatagc gaatcagtac gtagcacccc
    38761 ggattgaccc atttcgccgg aagaatttgc aaggccagca ggacaaacag gatcagcatc
    38821 ccgatgatgc tgcccggaat ggttaccggt agcagggaag caatgaaaat gcctgcatac
    38881 aggcaggcat aaatcaggac gaaagcgcgt aaatattgcc agataatgtt cagtgtctta
    38941 ctcataatca acaccccaga tgagatggat tcatcataca attaattcgt aaaacgtgct
    39001 accgatcaca ttaagaaatc agcgcttatt ttttcgctcc ccggtattac acaatgcgca
    39061 gcgaactcag gcatacatca agcatagaga gggattgcag tttgcagcaa tccctctttg
    39121 cagaccttta atccgttgac cggaggcgct aacatagcgc ctcatttttt tgcgagtgat
    39181 gatatgcgtg tgttactggc tccgatggag ggtgtacttg actctctggt gcgggaatta
    39241 ctgatcgaag ttaacgacta cgatctgtgc atcaccgagt ttgtccgcgt ggtggatcaa
    39301 ctgctgccag taaaagtctt tcatcgcatt tgccctgagc tacataatgc cagccggaca
    39361 ccatccggta cgctggtgcg cgtgcagttg ctggggcagt tcccacagtg gctggcagag
    39421 aacgccgccc gtgcggtgga gttaggttcc tggggcgtgg atcttaactg cggctgcccg
    39481 tcgaaaacgg ttaacggtag cggtggtggg gcaacgttac tgaaagatcc tgaactcatc
    39541 tatcagggtg caaaagcgat gcgcgaagct gtaccggcgc atttgcccgt cagcgtaaaa
    39601 gtgcgtctgg gctgggacag cggtgagaag aaatttgaaa tcgccgatgc ggttcaacag
    39661 gctggcgcta cggagctggt agtacatggg cggacaaaag agcagggtta ccgcgcggag
    39721 catattgact ggcaggcgat tggcgagatt cgccagcggt tgaatattcc tgtgattgcc
    39781 aacggtgaaa tctgggactg gcagagtgcg caacaatgca tgacgatcag cggatgcgac
    39841 gcagtgatga ttggtcgcgg ggcgctcaat attcccaacc tgagccgggt ggtaaaatat
    39901 aacgaaccgc gaatgccgtg gccggaggta gttgctttgc tgcaaaaata tacccgtctg
    39961 gaaaagcagg gcgataccgg gttatatcac gtagcgcgga ttaaacagtg gttgagttat
    40021 ttgcgtaaag aatacgatga agcaacagaa ttatttcagc atgttcggat gttgaataat
    40081 tcccctgata ttgcaagggc tattcaggcc attgatatcg agaaactcta agaaaccata
    40141 gtgatgtttt ttgacaatgt gttagctctt tatcaatagg ccgcacggtt gtttggtggc
    40201 gtcaaaatcg ccgaggcgtt ccctgaaggc cggggcagcc cacatggatg tgggctgagg
    40261 gcgcgtttta cagggatgtt acctcgcgcc cgacccggta gccgtaaggg ataagccgag
    40321 ggcaccgcgc agcggcgatt ttgttcgcca gagcccgggg gtgcaggggg cggcggcgac
    40381 tggtcgcccc ctgcgcgctc cttgcgccag tgattatatg aagcaatgca catgacagga
    40441 gcgcaacagg atgagagact gaaatattct cgttattttc ggatctgatg acgttaatta
    40501 attttaaaat atcatcttaa atacgccagt caccaccagt agcccaatca ggaaaataat
    40561 caatacagcc cagagtataa ttttcattgg cgttccttta tttgtgagtg ctgaataagt
    40621 ataggaaaaa acgaagccct ttgccgttat ccataaacga ttaagataaa tcggcgaata
    40681 tcattcactt agtgtatatt tttctggata tgtcgtttta ttgctactga tgtattgcca
    40741 cgcgaaacta aaaaatatat cgcaacacac ggtaatctcc gcgcaccttt tcttacctga
    40801 ttattccccc atgaatcgtg attcctttta tcccgccatc gcctgttttt cgctgttact
    40861 gatgctggcc gggtgtgcgc ctatgcatga aacccgccag gcgttaagcc agcaaacgcc
    40921 cgctgcacaa gttgacaccg tattacccac ggcgctgaaa aatggttggc cagacagcca
    40981 atggtggctg gggtatcacg ataatcaact cacttcctta attaataacg cactacagag
    41041 cgcaccggat atgcaggtgg ctgaacagcg cattcagctg gctgaagccc aggcgaaggc
    41101 agttgccact caggatggtc cgcagctcga cttttctgcg gatatggagc ggcaaaaaat
    41161 gtcggcagaa ggcttaatgg ggccgtttgc tctgaacgat ccggccgcag gtacgaccgg
    41221 tccgtggtac accaacggta cttttggctt aacggcgggc tggcatctcg atatctgggg
    41281 aaagaatcgg gcggaggtta ctgctcgcct gggtacggtt aaagcacggg cggcggaacg
    41341 cgagcaaacc cgccaattgc tggctggcag cgtagcccgt ctgtactggg agtggcaaac
    41401 ccaggcggcg ttaaacacgg tcttgcagca aatcgaaaaa gagcagaaca ccattatcgc
    41461 gaccgatcgc cagctatatc agaacgggat tacttcttca gttgaaggtg tggaaaccga
    41521 tattaatgcc agcaaaaccc ggcagcagct caatgatgtc gcgggtaaaa tgaaaattat
    41581 tgaggcacgg ttaaatgcac tcacaaatca ccagacaaag tcactgaagc ttaaaccggt
    41641 cgcgttgccg aaagtggcaa gccagcttcc tgatgaactg gggtactcct tactggcgcg
    41701 gcgggcagat ttgcaggcgg cgcactggta cgttgagtca tcgttaagca ctattgatgc
    41761 ggcaaaagcg gcattttatc ctgacatcaa cctgatggcc ttcctgcaac aggatgcgtt
    41821 gcacttaagc gatctgttcc gtcattccgc gcagcaaatg ggcgttacgg caggcctgac
    41881 gctacccatt ttcgatagtg gtcgtcttaa cgccaacctc gatatcgcaa aagccgaaag
    41941 caacttgtct atcgccagct acaacaaagc ggtggttgaa gcggtgaatg acgtggcgcg
    42001 ggcagccagt caggttcaga cactggcgga gaaaaaccag catcagtcgc aaattgagcg
    42061 cgatgccttg cgtgtagtag gtcttgcgca ggcgcgcttt aacgtgggcc tcattgctgg
    42121 ttcccgggtc agcgaagcca gaatcccggc gctgcgtgag cgggccaatg gcctgttatt
    42181 gcaagggcag tggctggatg cctccattca actcaccggt gcgctgggcg ggggttacaa
    42241 acgctgatga tatgcgcctt ctatacttaa catttattca gcgttaagtg gagaactcga
    42301 tggcacaggt cgcgataatt accgcctccg attcggggat cggcaaagag tgcgcgttat
    42361 tactggcgca gcaggggttt gatattggca ttacctggca ctcagatgaa gaaggggcaa
    42421 aagataccgc gcgtaaggtg gttagccacg gcgtacgtgc ggagatcgtg caactggatc
    42481 tcggcaatct accagaaggt gcgcaggcgc tggagaaact tattcaacgg ctggggcgta
    42541 ttgatgtgct ggtgaataat gcgggcgcaa tgaccaaagc gccgtttctt gatatggctt
    42601 tcgatgagtg gcgcaagatt tttaccgttg atgtcgatgg cgcattttta tgctcgcaaa
    42661 ttgcggctcg tcagatgttg aaacaagggc agggcggtcg catcatcaac attacatcgg
    42721 tacatgaaca tacgccgctg ccggatgcca gcgcctacac agccgctaaa catgcgctcg
    42781 gcgggttaac caaagcaatg gcgctggagt tggtcaggca taaaattttg gtgaacgcag
    42841 tcgcacctgg ggcgatcgcc acgccaatga acggcatgga tggcggcgac gtgaagcccg
    42901 acgcggagcc ttcgattccc ttgcgacgtt ttggcacaac gcatgagatt gccagcctgg
    42961 tggtatggct ttgttcggag ggcgcaaatt acaccaccgg gcagtcgttg atagtggatg
    43021 gcggctttat gttggcgaat ccgcagttta agccggagta gcgcagtgag gtgcgtggtt
    43081 atttcattgg gttaggccag ataagacacg ccagcgtcgc atcaggcatg gtgcaccgga
    43141 tgtcggatgc ggcgcgaaca ccttatccag ggcaatagtt ttacgcctga tgatccggct
    43201 ttttcttccc gcgccgtttc agccaccagc gcacgcctac caccagaacc acgaccagaa
    43261 tcaaccagac ccagtgcttc aaatgctggt cgagattgtg caaccacggc gcaatcacct
    43321 gcccaccagc gtaaccgata gtggtaaaaa tcagcgacca ggcaaatgcg ccgagaatat
    43381 tcagcggcag aaagattttc ggcggcagct ggctggcacc aatcagcgtc gggccaatga
    43441 cccgaaagcc atacataaaa cgcgtaccaa tgacaaacag atacggatgg cgttggataa
    43501 gtttctgcgc ccgctcaatt ttatcctgat gtttcgagaa acggcgtaac agcttgccgc
    43561 caaaccgccg cccgcacaga taaagcacct ggtcgccaat catgccgcca agcgccacag
    43621 aaagtaccac cagcgggaac tttaatagcc cctgatgtgc cgcaacgcct cccagcaaag
    43681 tcacggtttc accttccgcc aggctaccga tcaccagcgc ggcataacca tattgtgaga
    43741 tgagtgtatt gagatccata ggacagtctg tctccctttg cttgcctgta agcatacacc
    43801 ctgtgaatca ttcacgctga aaaaggacgt acatcagacg gtgcaaatta catgagtgaa
    43861 caaaaaataa tcacaagctg acttatactt gaactgtcag gttcatgaac tcacaacaag
    43921 ggggcggtat gagccatgtc tggggtctgt tttcccatcc cgatcgtgaa atgcaggtga
    43981 ttaatcgtga aaacgaaacg atttctcacc actacaccca ccacgttttg ctgatggcgg
    44041 cgattccggt gatttgcgcc ttcattggca ctacacagat tggctggaat tttggcgatg
    44101 gcactatcct gaagttatcc tggtttaccg gactggcgct ggctgtcttg ttttacggcg
    44161 ttatgctggc tggggttgcg gtgatggggc gggtcatctg gtggatggcg cgtaattatc
    44221 cgcagcgtcc gtcacttgcg cactgcatgg tcttcgcggg ctacgttgca actccgctgt
    44281 ttttaagcgg tctggtggcg ctttacccgc tggtctggtt gtgtgcgctg gtcggcacgg
    44341 tggcgctgtt ttacaccggc tacctgctgt atctcggtat tccgagcttc ctgaatatca
    44401 acaaagagga aggacttagc ttctccagtt cgacactggc gataggcgtg ctggtgctgg
    44461 aagtgctgct cgcccttacc gttatactat ggggctacgg ttatcgtttg ttctagaaag
    44521 ttcttttgcg ctgcattgct ggcgtaaatg tcagcaatgc agcatttcct cacgattctt
    44581 ctttgacgat cgcacttttt gctcgttatg atggcgtcgc tagcctcagt aaatccttac
    44641 tccggcggtg cgcaacccgt gcgcgtgaac cactatctga atgctcatca tgccgaaatt
    44701 tcgagtttct ttatttagcc tggccctgat gctggctgtg ccttttgcac cgcaggccgt
    44761 tgcgaaaacg gcagccgcta ccaccgcgtc acaaccggaa attgcctccg gtagcgcgat
    44821 gattgttgat ctgaatacca acaaagtgat ctattcgaac cacccggatt tggtgcgtcc
    44881 gattgcgtct atcagcaaat taatgaccgc gatggttgtg ctggatgcac gactgccgct
    44941 ggatgaaaaa ctaaaagtgg atatcagcca gacgccggag atgaaagggg tctattcgcg
    45001 cgtacgactg aacagcgaaa tcagccgtaa agatatgctg ttgctggcgc tgatgtcttc
    45061 agaaaaccgc gcggcggcaa gccttgcgca ccattatccc ggtggttaca aagcctttat
    45121 taaggcgatg aatgcgaaag cgaaatcgct cggaatgaac aacacgcgct ttgttgaacc
    45181 aaccggattg tcggtgcata acgtttcaac cgcccgtgac ttaaccaaac tacttattgc
    45241 cagcaaacaa tatccgttga tcgggcagtt aagtactacc cgggaagata tggcaacctt
    45301 ctctaacccg acgtatacgc tgccgttccg caatactaac catctggtgt atcgtgataa
    45361 ctggaatatt cagttaacca aaaccggttt taccaatgcg gcgggccatt gtctggtgat
    45421 gcgcacggtt atcaataata aaccggtggc gctggtagtg atggacgcgt ttggcaaata
    45481 tacccatttt gccgatgcca gccgcctgcg tacctggatt gaaaccggta aagtgatgcc
    45541 tgttccggca gcagcgttga gctataaaaa acaaaaagcc gcccaaatgg cggcggcggg
    45601 gcagacggca cagaacgatt aaatgaagta cggatggcga tactctgcca tccgtaattt
    45661 ttatttcact tcctgccagt ttttccgctt actggtttta ccaatccccg gattcatact
    45721 gttggtcgga tcgttctcgc gataaaactt ctgcaacggc gccttgtatg acaattcagc
    45781 gagtgaatat tttgaaaatt cagacaataa actgagcatt ctttttctct ctccatcatg
    45841 cttatggcaa actacggttt ttgcaccatt ttcgctgcca cgtcgggcaa caaaggaaga
    45901 aaaatccata tgaaatggct atgttcagta ggaatcgcgg tgagtctggc cctgcagcca
    45961 gcactggcgg atgatttatt cggcaaccat ccattaacgc ccgaagcgcg ggatgcgttc
    46021 gtcaccgaac tgcttaagaa aatgacagtt gatgagaaaa ttggtcagct gcgcttaatc
    46081 agcgtcggcc cggataaccc gaaagaggcg atccgcgaga tgatcaaaga cggtcaggtt
    46141 ggggcgattt tcaacaccgt aacccgtcag gatatccgcg ccatgcagga tcaggtgatg
    46201 gaattaagcc gcctgaaaat tcctcttttc tttgcttacg acgtgctgca cggtcagcgc
    46261 acggtgttcc cgattagtct cggtctggcc tcgtctttta acctcgatgc ggtgaaaaca
    46321 gtcgggcgtg tctctgctta tgaagcggca gatgatggcc tgaatatgac ctgggcaccg
    46381 atggtcgatg tctcgcgcga tccgcgctgg ggacgtgctt ccgaaggttt tggcgaagat
    46441 acgtatctca cctcaataat gggcaaaacc atggtggaag cgatgcaggg taaaagcccg
    46501 gcagatcgtt actcggtgat gaccagcgtc aaacactttg ccgcatacgg cgcggtagaa
    46561 ggcggtaaag agtacaacac cgtcgatatg agtccgcagc gcctgtttaa cgactatatg
    46621 ccgccgtaca aagcggggct ggatgcaggc agcggtgcgg tgatggtggc gctgaactcg
    46681 ctgaacggca cgccagccac ctccgactcc tggctgctga aagatgttct gcgcgaccag
    46741 tggggcttta aaggcatcac cgtttccgat cacggcgcaa tcaaagagct gattaaacat
    46801 ggcacggcgg cagacccaga ggatgcggta cgcgtggcgc tgaagtccgg aatcaacatg
    46861 agcatgagcg acgagtatta ctcgaagtat ctgcctgggt tgatcaaatc cggcaaagtg
    46921 acgatggaag agctggacga tgctgcccgc catgtactga acgttaaata tgatatggga
    46981 ttgtttaacg acccatacag ccatttgggg ccgaaagagt ctgacccggt ggataccaat
    47041 gccgaaagcc gcctgcaccg caaagaagcg cgtgaagtgg cgcgcgaaag cctggtgttg
    47101 ctgaaaaacc gtctcgaaac gctgccgctg aaaaaatctg caaccattgc ggtggttggc
    47161 ccgctggcag acagcaagcg tgacgtgatg ggaagctggt cggcggcagg tgtcgccgat
    47221 caatctgtta ctgtgctaac agggattaaa aacgccgtcg gtgagaacgg taaagtgctg
    47281 tatgccaaag gggcgaacgt taccagtgac aaaggcatta tcgatttcct gaatcagtat
    47341 gaagaggcgg tcaaagttga tccgcgttcg ccgcaagaga tgattgatga agcggtgcaa
    47401 accgcgaaac aatctgatgt ggtggtggct gtggtcggtg aagctcaggg gatggcgcac
    47461 gaagcttcca gtcgtaccga tatcactatt ccgcaaagcc aacgtgactt gattgcggcg
    47521 ctgaaagcca ccggtaaacc gctggtgctg gtgctgatga atgggcgtcc gctggcgctg
    47581 gtgaaagaag agcagcaggc tgatgcgatt ctggaaacct ggtttgcggg gactgaaggc
    47641 ggtaatgcaa ttgccgatgt attgtttggc gattataacc cgtccggcaa gctgccgatg
    47701 tccttcccgc gttctgtcgg gcagatcccg gtgtactaca gccatctgaa taccgggcgt
    47761 ccgtataatg ccgacaagcc gaacaaatac acttcgcgtt attttgatga agctaacggg
    47821 gcgctttatc cgttcggcta tgggctgagc tacaccactt tcaccgtctc tgatgtgaaa
    47881 ctttctgcgc cgaccatgaa gcgtgacggt aaagtgacgg ccagcgtgca ggtgacgaac
    47941 accggtaaac gcgaaggggc cacggtagta cagatgtact tgcaggatgt gacggcttcc
    48001 atgagtcgcc cggtgaagca gcttaaaggc tttgagaaaa tcaccctgaa accgggcgaa
    48061 actcagaccg tcagcttccc aatcgatatt gaggcgctga agttctggaa tcaacagatg
    48121 aaatatgacg ccgagcctgg caagttcaat gtcttcatcg gcactgattc cgcacgcgtt
    48181 aagaaaggcg agtttgagtt gttgtaattt tcgatgtttg tatgccggat aaggcgttta
    48241 cgccgcatcc ggcaagaaac gccgggcaaa atatgccttt taagtattta tctgattctg
    48301 caactgagtc cacccagtca ggctaactcc gccattatca actatgcttt tctcttaatt
    48361 cgctgaaaac aacagtaaaa gaatgaggaa agcatcatgc cactctcaaa ggtctgggca
    48421 ggttcactgg ttttgttggc agccgtgagc ctgccgctac aggcggcttc ccccgttaag
    48481 gttggttcaa aaatcgatac ggaaggcgcg ctgctcggca atatcatttt gcaggtgctg
    48541 gaaagtcacg gcgttcctac ggtaaataaa gtgcagctgg gaacgactcc cgtagtgcgc
    48601 ggggcgatca cttctggcga gctggatatc tatccggaat atactggcaa tggcgcgttt
    48661 ttctttaaag atgaaaacga tacggcatgg aaaaacgcgc agcaaggcta tgagaaagtc
    48721 aaaaaactcg atgcagagca aaacaagtta atctggctga cgcccgcgcc tgcaaataac
    48781 acctggacca tcgccgtgcg tcaggatgtg gcagagaaaa acaaactcac ttcgcttgcc
    48841 gagctgagtc gttatctgaa agagggtggt aacttcaaac tggcagcctc ggcagagttt
    48901 attgaacgcg ccgatgcctt acccgcgttt gaaaaagcct atggctttaa gctcggtcag
    48961 gatcagttgc tgtcactggc cggtggtgac acggcggtga cgatcaaagc cgctgcccag
    49021 caaacttctg gtgttaatgc cgcaatggct tacggcaccg acggcccggt tgcggcgctg
    49081 gggctgcaaa ccttaagcga tccgcaaggc gtgcaaccta tctacgcgcc tgcgccagtg
    49141 gtgcgtgagt cggtgctgaa agagtatccg caaatagcac agtggctaca gccagtcttc
    49201 gccagcctcg atgaaaaaac attgcagcag ctgaatgcca gcattgcagt ggaaggactg
    49261 gatgccaaaa aagtggcagc cgactacctg aaacaaaaag ggtggacgaa gtaatttccc
    49321 gtgacttatt tccgtattaa tcctgttctg gcgctgctgc tgttgctgac ggcaatcgca
    49381 gcggcgctgc cgtttatcag ttacgcacct aatcgtttag tttcgggtga ggggcgtcat
    49441 ctctggcagc tgtggccgca aactctctgg atgctggtgg gcgttggttg cgcctggctg
    49501 acagcctgtt ttattcccgc taaaaaaggc agcatttttg cactcattct ggcgcaattc
    49561 gtcttcgtat tgctggtgtg gggagctgga aaggcggcga cacaactggc gcaaaatggc
    49621 agtgcgctgg cgcgtaccag cctcggcagt ggtttctggc tggctgcggc gctggcactg
    49681 ctggcctgta gcgatgccat ccgccgaatc tccacgcatc cgctgtggcg ctggttgttg
    49741 catatgcaga ttgccattgt tccgctgtgg ttgctgtact ccggcacgct taacgatctc
    49801 tcgttaatga aagaatacgc caaccgtcag gatgtgtttg acgacgcgct ggcacaacat
    49861 ctgacgttgc tgtttggtgc gatgctgcct gcgttagtga ttggtgtgcc gttgggcatc
    49921 tggtgctact tttccaccgc gcggcaaggg gcgatttttt ctctgctgaa tgtcattcag
    49981 accgtgcctt cggtagcgct ctttggcctg ttgattgcgc cgcttgccgc gctggttacg
    50041 gcctttccgt ggctgggaac gctcggcata gcaggaaccg gaatgacacc cgctctgatt
    50101 gcactggtgc tctatgccct gttaccgctg gtgcgcggcg tagtagtcgg cttgaaccag
    50161 atcccgcgcg atgtgctgga gagcgccaga gcgatgggga tgagcgggac gcagcgtttc
    50221 ctgcatgttc agttaccgct ggcgttaccg gtatttctgc gcagcctgcg ggtggtgatg
    50281 gtgcaaaccg ttggcatggc ggtgattgcg gcgttaatcg gcgcaggcgg ttttggtgcg
    50341 ctggtttttc aggggctgtt aagcagtgcc attgatttag tgttgctggg ggtgatcccg
    50401 gtaattgttc tggcggtgct taccgacgcg ctgttcgatt tgcttatcgc actgctgaag
    50461 gtgaaacgta atgattgaat ttagccatgt cagcaaactg ttcggcgcac aaaaagccgt
    50521 taacgatctc aatctcaatt ttcaggaagg gagtttttcg gtactgattg gcacgtcggg
    50581 ttccggtaaa tccactaccc tgaaaatgat taaccgcctg gtggagcatg acagcggggt
    50641 gatccgcttt gccggagaag aaattcgctc gctgccagtg ctggagttgc gccgccggat
    50701 gggctatgcc attcaatcta ttggcctgtt tccccactgg agcgtggcac aaaacatcgc
    50761 caccgtgccg caattgcaaa aatggtcacg ggcgcggatc gacgatcgta tcgacgaatt
    50821 aatggcgcta ctggggctgg agccggattt gcgtgagcgt tatccgcatc agctttccgg
    50881 cggtcagcag caacgtgtgg gagtggcccg cgcgctggct gccgatccgc aagtcttgct
    50941 gatggatgaa ccattcggcg cgctggaccc ggtaacgcgt ggcgcgttgc aacaagagat
    51001 gacgcgcatt caccgtttgc aggggcgcac tattgtgctg gtcactcatg atattgatga
    51061 ggcgctacgg ctggcagaac atcttgtatt gatggatcac ggtgaagtgg tgcagcaggg
    51121 taatccgctg acgatgctga ctcgtccggc gaatgatttt gtccgccagt tttttggtcg
    51181 tagtgagctg ggtgtacgcc tgctttcgtt acgtagcgtg gcagattacg tgcgtcgcga
    51241 agaacgggca gaaggtgagg cgctggcaga agagatgacg ctacgcgatg cgctttctct
    51301 gtttgtcgcg cggggatgcg aggtgctgcc ggtggtgaac acgcagggcg agccttgcgg
    51361 cacgctgcat tttcaggatc tgctggagga ggcgtaatcg tatgaagatg ttgcgcgatc
    51421 cgctgttctg gctgattgct ctgtttgtgg cgctgatttt ctggctgcct tacagccagc
    51481 cgctgtttgc tgccttgttc ccacaactgc cacgacccgt ttatcagcag gaaagctttg
    51541 cagctctggc actggctcat ttctggctgg tgggaatttc gagtttgttt gcggtgatca
    51601 ttggtactgg tgccggaatt gctgtcactc gcccttgggg cgcggaattt cgcccactgg
    51661 tggaaactat tgccgccgtt gggcagactt ttccgcccgt cgcggtgctg gcgattgccg
    51721 ttccggtgat cggctttggt ctgaaaccag cgattatcgc cttgatcctt tacggcgtgc
    51781 tgcccgtcct gcaggcgaca cttgccgggc tgggagcgat tgatgccagc gtgacagaag
    51841 ttgcgaaagg tatgggaatg agtcgtggtc agcgactgcg taaggtcgag ctaccgctgg
    51901 cagctccggt gattctggcg ggtgtacgaa cttcggtgat tatcaacatt ggcacggcga
    51961 cgatcgcctc aacggtaggg gccaacacgc tgggtacgcc catcatcatc ggacttagcg
    52021 gatttaatac cgcgtatgtg atccagggag cgttactggt ggcactggcg gcgatcatcg
    52081 cagaccgcct gtttgaaagg ctggtgcagg cgttcagcca gcacgcaaaa taaaggtata
    52141 acctgcgagc atgacgccac caattccgcc taacgccata aacaggaaca gggcgatgac
    52201 cccaatttta tctatgcgca taatgcactc cttatgttaa cgaaaggatt gtacagtaaa
    52261 gcgtatttgt taacgaatca ttaaatgccg agtgggaaaa tatcatggcc ttgttcctgc
    52321 caactggtga gttgctgctg ttgggcggag gttcgattat caccgcacca taccagcaat
    52381 gtacggcctt cgaacagttc agggcgtagt tgattgagcg agtgggcgag gacatcaatg
    52441 cgccatcctt gttgactggc aatccagccc tccagccaca gacgagtggt atcctgaata
    52501 ttccagccaa ccaccagtgc atctttaccc tgttttttgc gcgccgaagc caggcaaata
    52561 gagatgtagt tgatcagtac gccgtcgagg atcgccagca gcgcctggag agtcggttgt
    52621 tggcactgaa gccgtcggcg cagaggaata aacagatgtg tggtgagtgt ctgggcgggg
    52681 taatcctgac cgcgctcttt gatccacgtt cgcaggctat gcagattgcc gctttgcagg
    52741 taagtcagta atgtttcttg ctgatcgcgc cagccgttct gcacatcaac attttcatta
    52801 ctgagcagca tttttacttt gctgacctgc acgccgttgt cgatccagcg tttgatctcg
    52861 cggatccggt caatatcggc atcgttgaac agccgatgac cgccgtctgt ccgttgcggt
    52921 ttcagcaatc cgtaacgcct ctgccacgcg cgtaacgtga cagggttaat atcacaaagc
    52981 aacgccactt caccaattgt gtaaagcgcc atcgtctcac ccttgctcgc gaggtcccgg
    53041 tttaacttta gacgcagttt tgcgaaccag gtagttttgc ccgttttttg tgcatctata
    53101 gggtgatttt atttttgcca ggcgattttg agtgatcgta ctcacgaatt ctcatttttc
    53161 tgcaagagtt caaagaaagt taaacgcaag caatgtatgt tacgcgtttt aaagggaagt
    53221 gtggtttgcg ggtatgtacg attttaatct ggtgttgctg ctgcttcagc agatgtgcgt
    53281 ttttttagtc attgcgtggt taatgagtaa aacgccatta ttcataccgt taatgcaggt
    53341 cacggttcgt ctgccgcata aatttctctg ctacatcgtc ttttccatct tctgcatcat
    53401 gggcacctgg tttgggttgc acattgacga ttctattgcc aatacccgtg cgataggtgc
    53461 ggtaatgggc ggcttactcg gcggtccggt cgtcggtggg ctggttggtc tgaccggcgg
    53521 gttacatcga tattcgatgg ggggcatgac cgcgctaagt tgcatgatct cgaccatcgt
    53581 tgaaggattg ctcggcggcc tggtacacag catcctgatc cgtcgcgggc gcactgataa
    53641 agtctttaac cccattaccg ccggtgccgt cacgttcgtc gctgaaatgg tgcaaatgtt
    53701 gatcatcctg gcgatcgccc gaccttatga agatgcggtg cgtctggtga gtaatattgc
    53761 tgcgccaatg atggtcacca ataccgtcgg cgcggcgttg tttatgcgta tattgctcga
    53821 taaacgcgcg atgtttgaaa aatacacttc ggctttttct gccactgcgc tgaaagtggc
    53881 ggcctcgacg gaaggcattt tgcgccaggg gtttaacgaa gtgaacagca tgaaagtggc
    53941 acaggtgctg tatcaggagc tggatattgg tgcagtcgcg attaccgatc gagagaaatt
    54001 gctggccttt accggaattg gtgacgacca ccatttaccc ggcaaaccaa tttcttcgac
    54061 ttatacctta aaagcgattg aaaccggtga agtggtctac gctgatggca acgaagtgcc
    54121 ttaccgttgc tctttgcatc cgcaatgcaa actggggtcg acgctggtca ttccgttgcg
    54181 tggtgaaaat cagcgagtga tgggtaccat caaattgtat gaagccaaaa accgtttatt
    54241 cagttcaatc aaccgcacgc tgggcgaggg gattgcgcaa ctgctttcgg cgcagatcct
    54301 cgccgggcaa tatgagcggc aaaaagcgat gctcacccag tcagagatca aactgcttca
    54361 cgcccaggtg aatccccatt ttttgtttaa tgcgcttaac accattaaag cggtgatccg
    54421 ccgcgacagc gaacaggcca gccagctggt gcagtatctt tccacttttt tccgcaaaaa
    54481 cttaaagcgg ccttcggagt ttgttactct cgccgacgaa attgaacatg tgaacgctta
    54541 tctgcaaatt gaaaaggcgc gcttccagtc gcggttgcag gtcaacattg ctattccgca
    54601 agaattatcc cagcagcaat tgcccgcgtt taccctgcaa ccgatagtgg aaaacgccat
    54661 taaacatggg acatcacaac tgctggatac agggcgagtg gcaatcagcg cccgacgtga
    54721 ggggcaacat ttgatgctgg agatcgaaga caatgccggt ttgtatcaac cggtaaccaa
    54781 tgccagtggg ctggggatga atctggtgga taagcgttta cgtgaacggt ttggcgatga
    54841 ctatgggata agcgtcgcct gtgagcctga tagttacacc cgaataacgt tacgactacc
    54901 atggagggac gaggcatgat taaagtctta attgtcgatg atgaaccgct tgcacgggag
    54961 aacctgcgcg tatttttgca ggagcagagc gatattgaaa tcgttggaga gtgttcaaac
    55021 gccgtagaag ggatcggcgc ggtgcataaa ctgcgcccgg atgtgctgtt tctcgatatc
    55081 cagatgccgc gcatcagtgg tctggaaatg gtggggatgc tcgacccgga acatcgcccg
    55141 tatattgttt ttctcaccgc gtttgacgaa tacgccatta aagcctttga agaacatgcc
    55201 tttgattatc tgctgaagcc aattgatgaa gcgcgactgg agaaaacgct ggcgcgattg
    55261 cgtctggagc gcagcaagca ggatgtttcg ctgttaccgg aaaatcaaca ggcgctgaaa
    55321 tttatccctt gtacggggca tagtcggatt tatttgctgc aaatgaaaga tgtggcattt
    55381 gtcagcagtc ggatgagcgg tgtctacgtt accagccacg aagggaaaga gggctttacc
    55441 gaattgacat tacgtaccct ggaaagtcgt acaccactac tgcgctgcca tcgtcagtat
    55501 ctggttaacc tcgcgcattt acaggagatc cgtctggaag ataacggcca ggccgagttg
    55561 attttgcgta atggcttaac cgtgccggtc agccgtcgtt atctgaaaag cttaaaagag
    55621 gcgattggcc tgtaaaagac tgctaaaatg gctttttgcc tcatcaacac ctgaaggcct
    55681 catgctaagt aacgatattc tgcgcagcgt gcgctacatt ttgaaagcca ataataatga
    55741 cctggtgcgt attctggcgc tgggtaatgt cgaagccacc gcggaacaga tcgccgtctg
    55801 gctacgtaaa gaagacgaag agggttttca gcgttgtccg gacattgttt tgtcttcatt
    55861 cctcaatggc ctgatttatg aaaaacgcgg caaggatgag tctgctccgg cactggagcc
    55921 ggaacgtcgc attaataaca acatcgtgct gaaaaaatta cgcatcgcgt tttcgctgaa
    55981 aaccgatgac attctggcga tcctcaccga acagcagttc cgcgtttcga tgccggaaat
    56041 tacagcgatg atgcgcgcac cggatcataa aaacttccgc gaatgcggcg atcaattttt
    56101 acgttatttt ctgcgtggac tggcagcgcg ccagcatgtg aagaaaagct aagacgggta
    56161 tggcggccat gcgaaacatg gccgccgaca gattatttca cttctttaaa accagcggct
    56221 ttcatcacca gttccatttg cgccatagtg ataccttttt tggcatcttc agcagaaacg
    56281 ttgattcctg aaataccctg cagggcttta aaatccactt tttccatatc aatagtcacg
    56341 ttttcctgcg cgtaggtatc ggtataggtt gatttttctt caacacccgc gatgtttttg
    56401 tatttagcgc ttaacggctc aagtgtcctg gcggcatctt ctttggtggt tgcaccaatg
    56461 gaggcaaatt gaattttggt ttcagaagat tgcttaagca ccttgtcacc tttgtagaca
    56521 taggtaatgg caatttcagt gccgttcaga ttggcgctga atttctttga ttcttctttg
    56581 tcaccgcagc cagcaagaga gaaaaccaga acagatgcaa caacaaggga aaacagctta
    56641 ttgaaagcct tcatgtaaaa ctccatttta tttaatcaag gaactggtga ctctcaccag
    56701 gggctatata ggatatgcct aatactgtgg cgtgagcagt ccggaacagg agtagaactc
    56761 ttaataaaaa gcactatttc atccttgttt ctgaagcatg gggaataatt gttcgcaaag
    56821 caaaacaccg ttattcattg cttctacccg tgcctcgctt tctgtattac gaaattgtcc
    56881 caacacatgt gccagccgat aaaaacccac cgcggtgagg tcattcgcca gcaactctgc
    56941 ctgaccaata gcactctgtt cctgatagcg ccagccgtta tggagcagtt gaataagtaa
    57001 cgcctggcag cgcatcagca actgatgagc ggtagacggc acaggcagaa cgctggcaga
    57061 aggtagcggt gccacaggcg cagtttctgc gtccagcgcc caggcgcggg tttttgtcat
    57121 catcacccgt ggttccagtg tcaattgccc ctctacaaaa ctgacaaagc cagaaaccag
    57181 gctcacgggg tcgtctgttt gttgcaaaag cgccgccatg cgttcaacgg caaaaggaga
    57241 acaggcagat gccggaaggg ataacgtcag cacattatct tccccttcgc cgctgattac
    57301 ctgcgcatcc agcgtctggc ggctgctatc ccaaccgagc gaaatacact ccgctatcgg
    57361 cagaataaat aagttatcga cctggttgat tggtcgtatg caggcggggg gacgctggcg
    57421 taaatattcc cgcaaagcca caatgcccgg ctggcgcaac ggtgcactca acatttgcca
    57481 ggcgtctggc gacagtggaa caacactact caagcggtta cgggtagcta acagcagctc
    57541 gccatcggca ctgcgttttg cagcttgtga aacaatttgc ccacctgcca gtgcgccagc
    57601 ctgaaaacta aacagccgac gttttgccgc gggagcgttt tcttgttcgc ttctcggcca
    57661 actacgcgaa aggtgcaaaa tactgccggt gtcgggatcg gtgaaccaga tgcgtaaacc
    57721 atactgctca atatcctgcc agcaacgcat acctaaagac accagccgca gatgatcaag
    57781 ctttgcttct ccggcaatgc cagcgccaac gaccgtgcgc cacggcacag gaggaacttc
    57841 accgatactg tcgcgccggg ccatctcttg tgcgcaattt aatcgactgt ttaatgccgc
    57901 aagctgacgt aagcattctc cggcatgata gtggctggcg cgggcgtgga aggcatcaac
    57961 gctggcgcgc agttgccgta gcgattcact cacccatcgc cagttgcagc gttccgccgt
    58021 ctgttgtgcg cggctgaaag cggcctcgta atggattggc ggctggctga tgccgcccag
    58081 ccataatgcc tgacttaatt gctgaacata ttgacgacac gcgttgccct cttcgctggc
    58141 aaacggatcg tcagatgatg tgacgtgttc gctgcgcatc tgccagatta aatgagtaaa
    58201 ttccgcttgc tgagttttgg cctcgacgaa ggcctgcaca gccagtgcta cgtgttcaca
    58261 cagcgttcct tcaatacaat cacagcgtgc gaaacgaatg ctgctgcggg aataaaaacg
    58321 tacatcgctc atcggtaatc gtgcagaggg aatttcgcca ggcgcacaga acaactcaat
    58381 ggtgatgcct ttagcgacca gtgtctgtgc gcgtttgcgg gtggcatcgg gcagggtagc
    58441 cagttcttcc agccagattg ccggatccca ctcttcttct ttttccgtag gctgggcggt
    58501 ggcacaaagt cgttgataac ttaacaccag catcacgcga tgacggcaca tgccgctggc
    58561 cccacaagtg cactgagcct ctttcagtgc ctgaccgttc gctaactggg tacggacacc
    58621 gtcactgaag gtggcgatta aagcgccgtt ctcatgactg atttccggga cgttgccatt
    58681 ttccagttcc ttaaggctgc gcttaacaaa accggcattg cttaacgccg tcagtgcctg
    58741 tggtgtcagt tctaataatt ccggacgtcg tgaattcatg actgaagatt ctccgcaagc
    58801 caggaagcca gctcgcccgg tgtcattgcg gctatttgtg cgccgacatt aaccagcgcc
    58861 tgggccgtat cgtggtcata gcaaggcgtt gcggtgctgt cgagcgctgc cagccccagc
    58921 actttgatgc cgctctggac acactttttc acctgatgcg tcagcaatga tgatgaccca
    58981 ccttcataaa aatcgctcac gagaataatg acactttttg caggttgttc aataagttgc
    59041 cgaccatact ccacggcact ggcgatattg gtcccgccgc ccagttgtac tttcattaat
    59101 aactctaccg gatcggcaac gtctgccgtg agatcaacca cgctggtgtc aaaagcgacc
    59161 agatgggtac gaatgccggg taactgccat aaacaggccg ccatcactgc ggagtggatc
    59221 accgaatcaa ccatcgatcc gctttgatca accagtagga ccagttgcca ttgttcgcta
    59281 tggcgtttaa tgcggctgtt aaagcggggg gattcgatat acaacttgcc gtgttgcggg
    59341 tgccagtgtt gcaggttggc gcgcagagta cttttgaaat caaagtttcg cgccagtgaa
    59401 ataaatgagc ggcgacggcg atcgcgtact ccagaaaaag cctgacgaac ttcctttgcc
    59461 agtcgcgcca taatctcttc aacaacctgg tgcactatcc ggcgggcggc agccagtact
    59521 tcgggattca tcagatgttt ggtgtgcaaa acggcgcgta gcaggctttc agaaggctgc
    59581 atacgttcca gcacgtcgag attcgtcacc acatcttcaa tgccgtagcg cagcacggca
    59641 tcgctttcca gccgctcaat cacctgttgc ggaaacagcg tgtgaatact gttgatccac
    59701 tcaggggtgg tgagatttga gtcacctaat ccaccggagc gttcaccacg ctggagccgt
    59761 tcaggatcgc gcccatacag ccactccagc gcgtggtcta tctgccgggc attgtcatcc
    59821 agcccacaaa gggtcgtttc tgccgcttcg ccaagaatta atcgccagcg ttgtagctca
    59881 cgggtggtca gaagatcgtt cagttcagac atggaaaact ccccagtgtt gcagcgatgc
    59941 cagtgcctgc tgttcgagtt gttgatgatg tgcaatgtct tgtggtggac aatgcaacgg
    60001 cattcgcagc gccgaaacgg gaagttgcac cagttgataa tgctcaagca cctgatgcgc
    60061 cagcgtcccg cgttctcgtg gtggtagcca ggccatcgcc gcgcgtaaat cgggcagggc
    60121 attgataaaa tcggcatcac tcagttgatt taaatgactg ctgaaaccgg cgataaatac
    60181 cggctgacag gccagttgat gacgggctaa tgccagcaaa ccgtgcagcg cctcgccgga
    60241 ttgtgccggg gataactgcg ccagcatcgt cagcgccgtt tcggcactgg cgttgggatg
    60301 tttcagacgc atcagtgcgc caagagtcgc gccgcgatcc agcgccgggg catgaatcgc
    60361 ctgactgcgg cgctccagta aggctactgc cgcagaaagc gaaacgccgg gtaaattaac
    60421 gccgctatgt agatcgcgca ggatatggca aagcgcctgc cagctatgca ggtgagtgtg
    60481 aaactccttt tcatccggtc tgccgttgga ttcacatagc cagagtgtgc gatcgatagc
    60541 cgcgcataac gtcgtttgta atatctgcgc gccttgcata ccgctgattt catccaaccg
    60601 ccataaggca tatagcactt ccagcgcctg gcccatttcg gcgaattgat tttcctgggc
    60661 gattaataat gtgagttgtt ccagtaattg ttgactgaaa gacgccagac ccgctaacgc
    60721 tgcctggcta agacatgtgg tgatacagcc gattccaccc gcgtccagca tatcggcttc
    60781 taatttatgg cgtgcggctt cctggagtgt ggcgccaaag caggcggcct caatcaatgc
    60841 agcatgctgg ctaagcgggc gggttaattt ccagcattct tcaccgttgc ctgcaagtga
    60901 cagtgtactt ccctgctggc gcacaacccc tgggatctcc agtattgcca accgatgtaa
    60961 cacctgactt tgcgccagtc catcgggggt aaagcgattt aaggtaagct ccgccggaag
    61021 agagattccg gtacgttcca gttcgcaggt gacatctttt ggcagaggcg gttgtggcgt
    61081 agaaggggaa agtttaccga atccgtcacc cgctaatgtg tctatcaacg ttaccagaat
    61141 cggatcggta tcgggattaa tgaccccgcg gtagctccac ggcaacggcg cgttcaaggc
    61201 ttctttaatc agcgaaccag ctatggcatc cagccagtca gtgcgtagag gtagtgtatg
    61261 gccgcgcaac tgtgccagtg ccatcgcatg cagatgagcg gcagccatat cagcggtaga
    61321 agcgggcaat ttgtgctggc gcaaacgggt aagaaccgtt ttcagcagtt gttcacctgc
    61381 ctgctgtaag ccacactgcc agcaccagtt ttgccagacc ggggcaggca ttccggaaag
    61441 gtatcctgcc agcacatcaa ggcgcttttc actgtagggt gtgagataac aacctgtaac
    61501 ggcatctgcc agcgagggca attctggctt gttaatttcc tgcgggcatt cgcgccacat
    61561 ttttgccagt gcaggagcgt gccagccgcc gcagacgact aacacgtcgc cattgttttg
    61621 ctgcatcgcc catgtaatcc agcgagccat aaaggcttcg cgctgacgat tgagagcatc
    61681 tccgggggaa tcgccccgca gttgggcaaa atagtgcgcc agcgtagagg gtaatgcagt
    61741 ttgctggctt tcatcttcga ataagtgatc ccacagggta tcgctgttat ccatgcaggt
    61801 ggcacgcagc agtaaggtct ggctgtcatc ttgcgtatcg ggtgattcgt cttcttcttc
    61861 actctgcgcc cagcaaggca aatcgatgaa gtaagtttgt gcctgaatac gacgtgcggc
    61921 ttgtagcgcc tgccactccg gcgaaaattc agcaaatggc gtccaggcac cacgcccggg
    61981 ggccgcaccg tcctgatact ggcaataact gtaaatagct accggaagct ggtgggctaa
    62041 aaacagttcg tccacccggt cattaaaatc agccgggcct tcaatcaata cgtatcgtgg
    62101 ccgctggctt tcgattaaca atttcaccag ccgggcgcag gccgggctat gatgccggat
    62161 gccgacaatt aacggctcgc tcatacatct ttcctcacgg caggcggtgg cgggcttgat
    62221 aataagcctg ccagtgagct tctttatgtg tagcaacacg ttgttcgaag taacggcgca
    62281 ggcgcgcgcg atcttcttcg ttatctttga cgatggttcc ggcaatacag tccaccagat
    62341 cggctggctc acctgctcga ttcgctaaaa accaggcgcg gacgcccacc gcgtgggcca
    62401 cgttcacggc ttcagcggtg gacataatcg ctgtcagtgt atccattgac gttttctttt
    62461 cgccgttggc acgcagatcg cgaaaggtgc ggaccagtaa ctccagtacc gcgtcaggca
    62521 ctttatgcgg aatgccgcta tgggccagca aacgtgccga agcactggcg acaagctcca
    62581 gctcctgggc gaaatccata atcggaaaga cagtttcaaa atcgaagcga cgctttagcg
    62641 cggcactcat ttcattgacg ccgcgatcgc gcgtattggc agtggcaata atattaaagc
    62701 cttcccgtgc atagagttga ctggcttcac cagtgagttc cggcaccgtc atcaccctgt
    62761 cagaaagcat acctaacaga cagtcttgca cttccagcgg tgtgcgcgtt atctcttcaa
    62821 aacggacgat tttgccgtcg cgcattccct ggtaaagcgg ggcagggacc agagcttccg
    62881 ttgacggacc atgattgata agcagagcgt agttccagcc gtacttgatt tgatcttcgg
    62941 tagtagatgc gccgccctgg atggttaacc cggcatcgcc gctaatagcg gttgccagta
    63001 attcagacag gagagatttt gcggtacccg gttcccccac caacatcaaa ccgcgcccgg
    63061 tggcaagagt gactaacatg cgttcgacag cagagggact aatcaccact ttacggcgaa
    63121 tattttgcgc ttcgtcgcca aggataaaag cacgggccgc aggcaaactt aactgccaac
    63181 caggcgggca aggagcgtta tcattttgtt ttaattttgc cagttcaacg gcgtataaca
    63241 cagcagcagg cggacgctgc aggtgattat tctgcggtga catatattca tccctgaaat
    63301 gtattaatga aataaacgat attttacgcg caataaatgt ttggcgcatt agttgtttga
    63361 gcggaaaata tctggaaagc agatcgcaaa aaataaaaaa acctgctggt gaggcaggtt
    63421 cttttattta caactttgac gttaagttac tgattatgca acatggcgcc ccaacgaacg
    63481 aattataacc acctacggac cccgttgcaa ttaagataat gagatttata atcctgactg
    63541 ttgggaagtt gcgccggatc gaaccaattt caagtcctgg ctaccggacg ggtggtgccg
    63601 caggtagtgc tctgatccgg agtgcaacat ggcgactcaa catacgtaaa ccagtatcga
    63661 caggcagtgt aagggtaaga tgctggtgtt gcgctcctta ataaggagag cgaatggaca
    63721 cgctgacaca aaagttaacc gtgctcattg ccgtactgga gttattagtg gctctgttac
    63781 ggttgattga tttgttgaag taacgggcaa attgaaacgc atttaagggg gaataccgtt
    63841 tcccccttta ataaagcttt acttgtcata ct
//
LOCUS       DAZFJL010000008        60499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFJL010000008 DAZFJL010000000
VERSION     DAZFJL010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.008"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            73..516
                     /gene="cmtB"
                     /locus_tag="ACHJ8C_000511"
     CDS             73..516
                     /gene="cmtB"
                     /locus_tag="ACHJ8C_000511"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO5550425.1"
                     /translation="MRLIDYFPESSISVIYAAKDWQEAIDFSMTSLLAINYVNESYIQ
                     AIKGSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLKQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLAGIISRG"
     gene            544..1932
                     /gene="cmtA"
                     /locus_tag="ACHJ8C_000512"
     CDS             544..1932
                     /gene="cmtA"
                     /locus_tag="ACHJ8C_000512"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO5550426.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGMIVGAEIPMFLGSM
                     IMGPLGGLVIKYIDKSLEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETKSEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1947..3224
                     /gene="yggP"
                     /locus_tag="ACHJ8C_000513"
     CDS             1947..3224
                     /gene="yggP"
                     /locus_tag="ACHJ8C_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO5550427.1"
                     /translation="MKTKVAAIYGKRDIRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QMLLPVELAASKGIELVYMNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LAWLVEKTHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3221..3536
                     /locus_tag="ACHJ8C_000514"
                     /pseudo
     CDS             3221..3536
                     /locus_tag="ACHJ8C_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
     gene            3558..4067
                     /gene="fumE"
                     /locus_tag="ACHJ8C_000515"
     CDS             3558..4067
                     /gene="fumE"
                     /locus_tag="ACHJ8C_000515"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO5550428.1"
                     /translation="MATLTEDDVLEQLNAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDITWDFISNVNSIIRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4064..4777
                     /locus_tag="ACHJ8C_000516"
     CDS             4064..4777
                     /locus_tag="ACHJ8C_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO5550429.1"
                     /translation="MKIELTVNGLKVQAQYSDDEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDVHQLRPFKGA
                     PETFNVAKLAENLCRVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFIKAPASALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLMD"
     gene            complement(4749..5426)
                     /locus_tag="ACHJ8C_000517"
     CDS             complement(4749..5426)
                     /locus_tag="ACHJ8C_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5550430.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTNAALQCCQLADTASAHPLDLHSAQRRMVAVASLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PSDDIHP"
     gene            complement(5420..6097)
                     /locus_tag="ACHJ8C_000518"
     CDS             complement(5420..6097)
                     /locus_tag="ACHJ8C_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5550431.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGRLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEKEIMARIDAALALTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQAREMLLQRLQHWALERGSLIILFERHHTPFLNHCQQAWQLQN
                     GALQPLC"
     gene            complement(6085..6792)
                     /locus_tag="ACHJ8C_000519"
     CDS             complement(6085..6792)
                     /locus_tag="ACHJ8C_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO5550432.1"
                     /translation="MHPFTSLTLWALAACTTLILPTQTILPVYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWAHAITLWLRILAIVSTSQL
                     WMQYVPVQRFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRINNRRTTLWAPADSTLQRVARY
                     AMILLMLAEFGAWIWLR"
     gene            complement(6793..7371)
                     /locus_tag="ACHJ8C_000520"
     CDS             complement(6793..7371)
                     /locus_tag="ACHJ8C_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO5550433.1"
                     /translation="MARRHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSIGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(7398..7826)
                     /locus_tag="ACHJ8C_000521"
     CDS             complement(7398..7826)
                     /locus_tag="ACHJ8C_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO5550434.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVLSQLRAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQENTALLSGKFEVIALNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGCLEELAFSRQSCALSGYDELHISPR"
     gene            8198..9217
                     /gene="epd"
                     /locus_tag="ACHJ8C_000522"
     CDS             8198..9217
                     /gene="epd"
                     /locus_tag="ACHJ8C_000522"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO5550435.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9267..10430
                     /gene="pgk"
                     /locus_tag="ACHJ8C_000523"
     CDS             9267..10430
                     /gene="pgk"
                     /locus_tag="ACHJ8C_000523"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO5550436.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            10645..11724
                     /gene="fbaA"
                     /locus_tag="ACHJ8C_000524"
     CDS             10645..11724
                     /gene="fbaA"
                     /locus_tag="ACHJ8C_000524"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO5550437.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKENEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            11915..12775
                     /gene="mscS"
                     /locus_tag="ACHJ8C_000525"
     CDS             11915..12775
                     /gene="mscS"
                     /locus_tag="ACHJ8C_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO5550438.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPARRNEFIIGVAYDSDIDQVKQILTDIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            12914..13549
                     /gene="argO"
                     /locus_tag="ACHJ8C_000526"
     CDS             12914..13549
                     /gene="argO"
                     /locus_tag="ACHJ8C_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO5550439.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            13642..14382
                     /gene="yggE"
                     /locus_tag="ACHJ8C_000527"
     CDS             13642..14382
                     /gene="yggE"
                     /locus_tag="ACHJ8C_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO5550440.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFNRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQPPAKTPAAH"
     gene            14540..15436
                     /gene="ygfI"
                     /locus_tag="ACHJ8C_000528"
     CDS             14540..15436
                     /gene="ygfI"
                     /locus_tag="ACHJ8C_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5550441.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTIQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVEWGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRYGTNH"
     gene            complement(15433..15585)
                     /locus_tag="ACHJ8C_000529"
                     /pseudo
     CDS             complement(15433..15585)
                     /locus_tag="ACHJ8C_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
     gene            complement(15610..16489)
                     /locus_tag="ACHJ8C_000530"
                     /pseudo
     CDS             complement(15610..16489)
                     /locus_tag="ACHJ8C_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
     gene            complement(16692..17585)
                     /gene="argP"
                     /locus_tag="ACHJ8C_000531"
     CDS             complement(16692..17585)
                     /gene="argP"
                     /locus_tag="ACHJ8C_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO5550442.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            17726..17956
                     /locus_tag="ACHJ8C_000532"
     CDS             17726..17956
                     /locus_tag="ACHJ8C_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5550443.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RPLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            18012..18671
                     /gene="rpiA"
                     /locus_tag="ACHJ8C_000533"
     CDS             18012..18671
                     /gene="rpiA"
                     /locus_tag="ACHJ8C_000533"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO5550444.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            18927..20159
                     /gene="serA"
                     /locus_tag="ACHJ8C_000534"
     CDS             18927..20159
                     /gene="serA"
                     /locus_tag="ACHJ8C_000534"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO5550445.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            20388..20447
                     /gene="ibsC"
                     /locus_tag="ACHJ8C_000535"
     CDS             20388..20447
                     /gene="ibsC"
                     /locus_tag="ACHJ8C_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO5550446.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            complement(20548..21150)
                     /gene="fau"
                     /locus_tag="ACHJ8C_000536"
     CDS             complement(20548..21150)
                     /gene="fau"
                     /locus_tag="ACHJ8C_000536"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO5550447.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRTLTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(21171..21354)
                     /gene="ssrS"
                     /locus_tag="ACHJ8C_000537"
     ncRNA           complement(21171..21354)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHJ8C_000537"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(21396..21725)
                     /gene="zapA"
                     /locus_tag="ACHJ8C_000538"
     CDS             complement(21396..21725)
                     /gene="zapA"
                     /locus_tag="ACHJ8C_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO5550448.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            21893..22471
                     /gene="ygfB"
                     /locus_tag="ACHJ8C_000539"
     CDS             21893..22471
                     /gene="ygfB"
                     /locus_tag="ACHJ8C_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO5550449.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            22497..23822
                     /gene="pepP"
                     /locus_tag="ACHJ8C_000540"
     CDS             22497..23822
                     /gene="pepP"
                     /locus_tag="ACHJ8C_000540"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO5550450.1"
                     /translation="MSDISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSIQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            23819..24997
                     /gene="ubiH"
                     /locus_tag="ACHJ8C_000541"
                     /gene_synonym="visB"
     CDS             23819..24997
                     /gene="ubiH"
                     /locus_tag="ACHJ8C_000541"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO5550451.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATVCGVDWQQDPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAAKP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            25020..26222
                     /gene="ubiI"
                     /locus_tag="ACHJ8C_000542"
                     /gene_synonym="visC"
     CDS             25020..26222
                     /gene="ubiI"
                     /locus_tag="ACHJ8C_000542"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO5550452.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRAPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRACCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESERQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(26296..26379)
                     /locus_tag="ACHJ8C_000543"
     CDS             complement(26296..26379)
                     /locus_tag="ACHJ8C_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5550453.1"
                     /translation="MSKNTKSKIHNIRKYNAKTEVKLVYFK"
     gene            26670..27764
                     /gene="gcvT"
                     /locus_tag="ACHJ8C_000544"
     CDS             26670..27764
                     /gene="gcvT"
                     /locus_tag="ACHJ8C_000544"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO5550454.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEAQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            27788..28177
                     /gene="gcvH"
                     /locus_tag="ACHJ8C_000545"
     CDS             27788..28177
                     /gene="gcvH"
                     /locus_tag="ACHJ8C_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO5550455.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            28295..31168
                     /gene="gcvP"
                     /locus_tag="ACHJ8C_000546"
     CDS             28295..31168
                     /gene="gcvP"
                     /locus_tag="ACHJ8C_000546"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO5550456.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQAAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(31334..32767)
                     /gene="bglA"
                     /locus_tag="ACHJ8C_000547"
     CDS             complement(31334..32767)
                     /gene="bglA"
                     /locus_tag="ACHJ8C_000547"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO5550457.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVVPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMIDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAAHRINPEMKVGCMLAMVPLYPYACNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEDDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            32812..33123
                     /gene="yqfB"
                     /locus_tag="ACHJ8C_000548"
     CDS             32812..33123
                     /gene="yqfB"
                     /locus_tag="ACHJ8C_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO5550458.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDEFESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPDQTQFYVIEFKC
                     L"
     gene            33287..33946
                     /gene="yqfA"
                     /locus_tag="ACHJ8C_000549"
     CDS             33287..33946
                     /gene="yqfA"
                     /locus_tag="ACHJ8C_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO5550459.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(34023..35003)
                     /gene="ygfZ"
                     /locus_tag="ACHJ8C_000550"
     CDS             complement(34023..35003)
                     /gene="ygfZ"
                     /locus_tag="ACHJ8C_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO5550460.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEYQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLKGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVCDDANTLRIEPLPYSLEE"
     gene            35246..35512
                     /gene="sdhE"
                     /locus_tag="ACHJ8C_000551"
     CDS             35246..35512
                     /gene="sdhE"
                     /locus_tag="ACHJ8C_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO5550461.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            35493..35900
                     /gene="ygfX"
                     /locus_tag="ACHJ8C_000552"
     CDS             35493..35900
                     /gene="ygfX"
                     /locus_tag="ACHJ8C_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO5550462.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLCLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(35940..36461)
                     /gene="fldB"
                     /locus_tag="ACHJ8C_000553"
     CDS             complement(35940..36461)
                     /gene="fldB"
                     /locus_tag="ACHJ8C_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO5550463.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            36573..37469
                     /gene="xerD"
                     /locus_tag="ACHJ8C_000554"
     CDS             36573..37469
                     /gene="xerD"
                     /locus_tag="ACHJ8C_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO5550464.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            37494..38204
                     /gene="dsbC"
                     /locus_tag="ACHJ8C_000555"
     CDS             37494..38204
                     /gene="dsbC"
                     /locus_tag="ACHJ8C_000555"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO5550465.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            38210..39943
                     /gene="recJ"
                     /locus_tag="ACHJ8C_000556"
     CDS             38210..39943
                     /gene="recJ"
                     /locus_tag="ACHJ8C_000556"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO5550466.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDPQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDVLERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            40034..41132
                     /gene="prfB"
                     /locus_tag="ACHJ8C_000557"
     CDS             join(40034..40108,40110..41132)
                     /gene="prfB"
                     /locus_tag="ACHJ8C_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO5550467.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            41142..42659
                     /gene="lysS"
                     /locus_tag="ACHJ8C_000558"
     CDS             41142..42659
                     /gene="lysS"
                     /locus_tag="ACHJ8C_000558"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO5550468.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(42702..43250)
                     /gene="idi"
                     /locus_tag="ACHJ8C_000559"
     CDS             complement(42702..43250)
                     /gene="idi"
                     /locus_tag="ACHJ8C_000559"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO5550469.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEEAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLAAVLRGIDATPWAFSP
                     WMVMQATNREARKRLSAFTQLK"
     gene            43305..43376
                     /locus_tag="ACHJ8C_000560"
     CDS             43305..43376
                     /locus_tag="ACHJ8C_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5550470.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(43373..43498)
                     /gene="yqfG"
                     /locus_tag="ACHJ8C_000561"
     CDS             complement(43373..43498)
                     /gene="yqfG"
                     /locus_tag="ACHJ8C_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO5550471.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(43500..44948)
                     /gene="uacT"
                     /locus_tag="ACHJ8C_000562"
     CDS             complement(43500..44948)
                     /gene="uacT"
                     /locus_tag="ACHJ8C_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO5550472.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSQDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     IASGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            45384..47303
                     /gene="ygfT"
                     /locus_tag="ACHJ8C_000563"
     CDS             45384..47303
                     /gene="ygfT"
                     /locus_tag="ACHJ8C_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO5550473.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIDVCPTQALRLMDDKGLQQIKVARQRKTAAGKALS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDISFNEL
                     TAEYDAVFLGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESAEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            47303..47791
                     /gene="ygfS"
                     /locus_tag="ACHJ8C_000564"
     CDS             47303..47791
                     /gene="ygfS"
                     /locus_tag="ACHJ8C_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO5550474.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDCISAPVMCHQCENAPCVGACPVGALTMGEQVVQANSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIAASGEN
                     PL"
     gene            complement(47827..49194)
                     /gene="ghxQ"
                     /locus_tag="ACHJ8C_000565"
                     /gene_synonym="ygfQ"
     CDS             complement(47827..49194)
                     /gene="ghxQ"
                     /locus_tag="ACHJ8C_000565"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO5550475.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLMILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(49230..50546)
                     /gene="guaD"
                     /locus_tag="ACHJ8C_000566"
     CDS             complement(49230..50546)
                     /gene="guaD"
                     /locus_tag="ACHJ8C_000566"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO5550476.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFIDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(50564..51964)
                     /gene="xanQ"
                     /locus_tag="ACHJ8C_000567"
     CDS             complement(50564..51964)
                     /gene="xanQ"
                     /locus_tag="ACHJ8C_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO5550477.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSGMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(52129..54999)
                     /gene="xdhD"
                     /locus_tag="ACHJ8C_000568"
     CDS             complement(52129..54999)
                     /gene="xdhD"
                     /locus_tag="ACHJ8C_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO5550478.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEDIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGEIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(54996..55775)
                     /gene="ygfM"
                     /locus_tag="ACHJ8C_000569"
     CDS             complement(54996..55775)
                     /gene="ygfM"
                     /locus_tag="ACHJ8C_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO5550479.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWIDWDNGALRIGAMSRLQSLRDARFIPAALREALGFVYSRHIRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAITDHDGMRIALDGVASKALRLHDVETQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(55826..57154)
                     /gene="ssnA"
                     /locus_tag="ACHJ8C_000570"
     CDS             complement(55826..57154)
                     /gene="ssnA"
                     /locus_tag="ACHJ8C_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO5550480.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIDDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     SGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKVAASMWRRMDALA"
     gene            complement(57157..60255)
                     /gene="ygfK"
                     /locus_tag="ACHJ8C_000571"
     CDS             complement(57157..60255)
                     /gene="ygfK"
                     /locus_tag="ACHJ8C_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO5550481.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDALNKLLQDEAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWELNHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTTLKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVELRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISVTLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPDFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
BASE COUNT        14720 a        15070 c        16065 g        14644 t
ORIGIN      
        1 ctttcaaatt tttacgcggg tattttattt ttaccgctgg acggtaccct aattatacaa
       61 taaggataat ttatgcggct tattgattat tttccagagt catctatctc agttatatat
      121 gcagcaaaag actggcagga agctatcgat ttttcaatga catcattgtt agcaataaat
      181 tatgtcaacg agagctacat tcaggcaatt aaaggttcca ccattaacaa tggcccttat
      241 tatattctcg caccaggcgt ggcaatgcct catgcgcgac cggaatgtgg agcgcttaaa
      301 accgggatgt ctttgacgtt acttaaacaa ggcgtttatt ttccggggaa tgacgaaccg
      361 attaaattac tcatcggact ttctgctgcc gatgccgatt cgcacattgg tgctattcag
      421 gcgttaagtg aattactgtg cgaagaagaa atactcgaac aactcttaac cgcatcatca
      481 gaaaaacaat tagcgggcat tatcagccgc ggataattac tctctcctct acaggataat
      541 attatggaaa acaagtctgc tcgtgcaaag gtccaggctt ttgggggctt tttgactgca
      601 atggtcatcc ccaatattgg tgcttttatt gcctggggtt ttataacggc attatttatt
      661 cccaccggct ggctgccgaa tgaacatttc gccaaaattg ttggtccgat gattacctat
      721 ttattgcccg tgatgattgg ttctaccggt ggtcatctgg tcggcggtaa acgcggcgcg
      781 gtcatgggcg gaattggtac gattggtatg atcgtcggcg ctgaaatccc gatgttcctt
      841 ggctcgatga ttatggggcc gctcggtggt ctggtgatta aatatatcga caagtcgctg
      901 gaaaaacgca tacctgccgg ttttgagatg gttatcaata acttctcatt aggtatcgcg
      961 gggatgctcc tttgtctgct gggttttgaa gtgatcggcc cggcggtgtt aattgctaat
     1021 actttcgtca aagagtgtat tgaggcgctg gtacatgcgg gttatctacc tttgctgtca
     1081 gtcatcaatg aaccggcgaa agtgcttttc cttaataatg cgatcgatca gggcgtctat
     1141 tatccgctgg gaatgcaaca ggcttcggtt aacggtaaat ccatcttctt catggtggcc
     1201 tccaatccag gtcctggtct ggggctgctg ctggcgttta ccttgtttgg taaagggatg
     1261 agtaaacgtt ctgcgcccgg ggcgatgatt attcacttcc tcggtgggat ccacgaactg
     1321 tatttcccgt atgtgctgat gaagccgctg accattattg ccatgattgc gggcggtatg
     1381 tctggcacct ggatgtttaa cttactggac ggtggtctgg tggctggccc aagtccgggg
     1441 tctatctttg cttacctggc actgacgccg aaaggctcgt tcctggcgac aattgccggt
     1501 gttacggtag gtaccctggt gtcctttgct attacttcgc tgatactgaa gatggaaaaa
     1561 acggtggaaa cgaagagcga agatgagttt gctcagtcag ccaatgcggt taaggcgatg
     1621 aaacaagaag gtgcattctc gttaagccgt gttaagcgta ttgcctttgt ttgcgatgca
     1681 gggatgggct ccagtgcgat gggcgcaacc accttccgta aacgcctgga aaaagcgggg
     1741 ctggcaatag aagtaaaaca ttacgccata gaaaacgtgc ctgcggatgc ggatatcgtc
     1801 gttactcacg ccagtctgga agggcgcgtg aaacgtgtga cggataaacc gctgatactg
     1861 attaataact atattggcga tccaaaactc gacactttat ttaatcaatt aaccgccgaa
     1921 cataaacact gattacagag gtaaaaatga aaaccaaagt tgctgctatt tatggcaagc
     1981 gggatatccg tctgcgcgaa tttgaactgc cagaaattac cgataatgaa ctgctggtga
     2041 gtgtaatttc tgacagcgtc tgtttatcga cctggaaagc ggcgttactc ggtagtgaac
     2101 ataaacgcgt acccgacgat ttagaaaatc atccggtcat taccgggcat gaatgtgccg
     2161 gggttattgt cgaagtgggt aaaaatctca ctggcaaata taaaaaaggc cagcgttttg
     2221 tattgcaacc ggcgatgggg ttaccaagcg gatattcagc gggctacagc tacgaatatt
     2281 ttggcggtaa cgccacctat atgattattc ccgaaatcgc cattaatttg ggctgcgtat
     2341 taccctatca cggctcttat tttgctgcgg cgtcgctggc agagcctatg tgctgcatta
     2401 ttggcgctta tcatgccaat tatcacacca cgcaatatgt ttatgagcat cgtatgggcg
     2461 tcaaacctgg cggcaatatt gcgctactgg cgtgtgcagg cccgatggga attggcgcta
     2521 ttgattacgc cattaacggc ggcatacaac cgtcgcgggt ggtggtagtc gatatcgacg
     2581 acaaacggct ggcgcaggtg cagatgctgc tgccggtgga actggcggcc agtaaaggca
     2641 ttgagctggt gtatatgaat actaaaggga tgagcgatcc ggtccagacg ctgcgggcgt
     2701 tgacggatga tgccggattt gatgacgtat ttgtctatgc agcggtgcct tcagtcgtcg
     2761 agatggcgga tgaattactg gcggaagatg gctgcctgaa cttctttgcc ggtccgacgg
     2821 ataaaaactt caaagtgccg tttaatttct acaacgtcca ttacaacagt acgcacgttg
     2881 tcggcacgtc cggcggttcc acggacgaca tgaaagaggc gattgccctt agcgccacgg
     2941 ggcagttaca gccgtcgttt atggtgaccc atatcggtgg cctggatgcg gtgccagaaa
     3001 ccgtgctcaa tctgccggat atccctggcg gtaaaaaact catttataac ggcgtgacca
     3061 tgccgctgac cgccattgcc gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag
     3121 agctggcgtg gctggttgag aaaacgcacg gtatctggaa tgaacaggcc gagaaatatc
     3181 tgctggcaca atttggcgtt gatattgggg aggccgcgca atgatgtccc tggcgtggcc
     3241 attatttcgc gttacggaac aggcagcgtt ggctgcctgg ccgcaaaccg gctgtggcga
     3301 caaaaataaa attgatggcc ttgcggtcac cgcgatgcgc caggcattaa acgacgttgc
     3361 ttttcgtggg cgagtggtta tcggcgaggg ggaacgatat cctctttagc gctacgggcg
     3421 tgacgggcgg cgagttggtg aacggtatcc aacagacggc gaatggggtg cggacgcaga
     3481 cattactgat cggcggcgcg gaccaaacgt gtaatataat agactccctg cattgatggt
     3541 gatctatcgg aagaccaatg gcgacgctga cagaagatga tgtgcttgag caactgaatg
     3601 cacaggacaa tttattttca tttatgaaaa ctgcgcattc tattttgctc caggggatac
     3661 gccagtttct gccgtcgctg tttgtcgata acgatgaaga gatcgtcgaa tatgcagtga
     3721 agccgttact cgcccaaagc ggcccgcttg acgatattga tgttgcgctg cgtttgattt
     3781 atgcgctggg gaaaatggat aaatggctgt acgccgatat cacgcatttt tcccagtttt
     3841 ggcattacct gaacgaacag gatgaaacgc ccggatttgc tgatgacata acctgggatt
     3901 ttatcagcaa cgtcaatagc ataatccgca atgcgacgct ctatgacgcg ttgaaagcga
     3961 tgaagttcgc cgatttcgcc gtctggtcag aggcgcgttt tagcgggatg gtcaaaacgg
     4021 cgctgacgct ggcagtaacg accaccttaa aggaattaac gccgtgaaaa tcgaactgac
     4081 ggtgaatgga ctaaaggtac aggcgcagta ctctgatgat gaaattgaaa atgtacataa
     4141 accattgctg cgtatgctgg cggccttaca gacagtaaat ccgcagcggc gtacggtggt
     4201 ttttctttgc gctccgccgg ggacgggtaa atctacccta accaccttct gggaatatct
     4261 cgcgcaacaa gacccggaac tgcccgctat tcagacgctg ccgatggatg gttttcacca
     4321 ttacaatagc tggctggatg tgcatcaatt gcgacccttc aaaggcgcac cagagacatt
     4381 caacgttgcg aaactggcgg aaaatctgtg ccgggttgtg gaaggggatt gtacgtggcc
     4441 gcagtacgat cgacaaaagc atgatcctgt tgaagatgcg ctgcacgtta ccgcaccact
     4501 cgtcatcgtc gaaggaaact ggttgttgct ggatgatgaa aagtggtgcc agcttgcgca
     4561 attttgtgat ttctcgatct ttatcaaagc tcccgcatct gcgttgcggg agcgtctggt
     4621 ggggcgcaag ctggcgggag ggttatcgct ggcagacgca gaagcctttt atgaccgtac
     4681 cgacgggccg aatgttcgcc gggtgctgga agagagtctg cctgcaaatt tgaccttaat
     4741 gatgaccgct acgggtgaat atcgtctgat ggattaacgt tcctgattac accatcttcc
     4801 agtcgcacca cacgagagaa atgacgccgg gtaaatgcgg cgtcatgact gattgctacg
     4861 acgctcgtgc ctcgctggcg gcacttttcc agccagcttt caaacacgct caaccaattt
     4921 tcgtcaaaat cccgactcgg ctcgtccagt agcaataggg gtggagagag tgcttccagg
     4981 ctggcgacgg cgaccatccg tcgctgcgca ctatgcagat ctaacggatg cgcgctggca
     5041 gtgtctgcta actgacagca ttgcagggca gcattggtac gttgagtgat ttcatccgcc
     5101 gggcattttt gcagtttcaa accaaaggcg atctcgtcag caacggtgct gtgaaaaagc
     5161 tggttttccg cttcctgaaa cagtacgcca atctttgccg cgcgctggcg gtttttcagg
     5221 tttttcatcg cttgttgttg cagcataacc gtaccggctg tgggcgtaag aagacctgcc
     5281 attactcgta acagcgtcga tttccctgcg ccattatcgc cggtcagcgc cagccattca
     5341 ccttgtttga gttgcagaga aatgtcgcaa agacaatccg ttgccgcgcc aggccagcga
     5401 taagatatct gatttaacgt taacatagtg gctggagagc tccgttttgc aattgccacg
     5461 cttgctggca gtggttcagg aaaggtgtgt gatgacgttc aaaaagaata atcagcgaac
     5521 cacgttcaag cgcccagtgt tgtaagcgct gcaacagcat ttcgcgggcc tgcggcgtca
     5581 ggcggctaaa ggcttcatca agaattaaga gctttggctg catcgcgatg gcgcaggcga
     5641 tgaccacacg ctgtgtctct ccaccggaga gtgttgccgg gtgacgatgg cgcaacggct
     5701 ggcactcggt cagggccagg gcggcgtcaa tccgcgccat aatctctttt tcagcaagac
     5761 acagattttc tggcccaaag gcaacttctt cttccacgct aaaggtacag ccggagagct
     5821 gcaaataggg cgattgctgg acgagctgga tggtggcgga ttgttcatta agcgggagac
     5881 gcccaatggg cgtaccgaga agcgtccccg tgccggttat ctctcctggt agaaagtccg
     5941 gataccagcc cgccatcagc tgcgccagcg tacttttgcc actgccgttg tcaccaaaga
     6001 tggcgaccat ccccggtttg acatagtgaa agtcataaca gaacggagga tgcgttgagt
     6061 gtgtcgggca atagcgaaac tgttctaacg taaccatatc cacgctccga attcagccag
     6121 cattagcaat atcatggcgt aacgtgctac gcgttgtagg gtgctatcgg ctggtgccca
     6181 taacgtagtg cgcctattgt tgattcgaaa tgcccgcata tccagcgccg caccacggac
     6241 agcaagatcg tttagcgcat tgtgtgttag cggaataatt aacgcgggca tcgcacgtaa
     6301 acgctgatac cagccctcat ccagcggcac gccgcgggcg cgctgcgctt catgaataat
     6361 cgccaattgc cgttttaact gctcaacgac caataacggc ccggcaaaca gataagcgac
     6421 gcccggcggc aggcgagaag caaacaaagc gcggataaaa cgttgcacgg ggacgtactg
     6481 catccacaac tgtgaggttg aaacaatcgc cagaatccgc aaccagagcg taatagcatg
     6541 cgcccaccgt tctggagagc gtggcgtgcc gcttagccat tctgtcagcc agccaccgtg
     6601 caccagccac agtccagccc ccagcgagaa catcagccag acgacatatt ttgcccgccg
     6661 ccgcgtggcc tttagcgcaa ttaggcagaa aaaggtcgcc gcgctataga ctggcaatat
     6721 tgtttgtgtg ggcaggataa gcgtggtgca ggccgccagc gcccacagcg ttaaggatgt
     6781 aaacggatgc atttagcgca cggcagccat tgccggaaaa tggcgcgtgg tgcgaatcgg
     6841 cagctgacgc agtaaaagcc agacaatcac cgctgtaaga attttatcga ccaggttcgc
     6901 gcctatcacc gtaatcgcca ccgactccac caagttttgc cctatcgagt gcatccaggc
     6961 aacgaacaaa tcggctccgc ttcctgtcac gccgccgaac agcgcagtac gcagcggtac
     7021 agcgacgacc gttaccgcaa gcgtaataat cacgccgctg acaacgactt ttggcagggt
     7081 gcgaaaccaa cccgctcgcg ccagccaacc ggcgaccagg ccgatgacca tcgcgacggg
     7141 cgcaaatgcg gcggcgatcg ggtcggtcag caatccccag agtaaattgg tcagtaaacc
     7201 ggtcagcatc ccgatcaccg gaccaagcag taccgcgcta ataagtgtgc cgatggagtc
     7261 gagaaaaata ggtaatttga ccatgctggc cagttgtccg ccgatcatat tgatggcgat
     7321 agagataacg atcaacacca atgcctgact ggaaaagtgg cgacgcgcca taagaaaacc
     7381 tcactgaaag tagttattta acggggggaa atatgcaact cgtcgtaacc agaaagcgcg
     7441 caagactggc ggctaaacgc cagttcctca aggcagccga tcaccagttc aagcgtggtc
     7501 cgcacggtac agccgggcat catatgaccg ccaagcatcg tgccgtgtgg atcggaaacg
     7561 cagagatgaa gatgttcacc actctgttcc agcgtgccgt tcaatgcgat aacttcgaac
     7621 ttaccgctta gcagggcagt gttttcttgc ccggcatagc gtaaggcgac gtcagtcaga
     7681 ctgccggtac aacctgcaat ccacgcggcg tggagttgtt gttgctgcgc gaaggcgcgt
     7741 aattgggaga gaacttcctg cccggggagc aggcgcagcg cgtaaaaacg tgcggaggaa
     7801 gaatatgggt agggcaatgt catcattgga gtactccgaa actcggacgt attgcggata
     7861 atatcgacgc tgcatcagtc atattctgat ataagtcagg gtttgtgacg gcacaacgaa
     7921 aagattttgc tacgcttatt cgtgattctg caaagtggaa taaattccac aggggcgggg
     7981 ctgaaaacgt gacgtaagtc agataattgt cttctttgac tggacaatca ttccttttat
     8041 tccacgtttc gcttatccta gctgaagcgt ttcagtcgat taaatgttcg acaattaacc
     8101 aatcagtcgc agtttgcgac aggtaaggtt tccccgaacg atttgctgga ttactctgtc
     8161 tggcaaattt gctcagggaa aaccttgcag gagatctatg accgtacgcg tagcgataaa
     8221 tggcttcggt cgcatcgggc gtaatgtggt tcgtgctttg tatgaatccg gacgccgtgc
     8281 ggaaattacc gtggtggcaa tcaacgaact ggcggatgct gcgggcatgg cgcatttgtt
     8341 gaaatatgac accagccatg gccgttttgc atgggaagta cgacaggaac gcgatcaact
     8401 ttttgttggt gatgacgcca tccgtgtatt gcatgaacgt tcactgcaat cgctcccctg
     8461 gcgtgaactt ggcgttgatg tagtcctcga ctgcaccggc gtatatggct cccgcgagca
     8521 tggtgaagca cacattgccg ccggggctaa aaaagtgctc ttttcacatc ctggcagtaa
     8581 cgatctcgac gcgaccgttg tttatggcgt caatcaggat caacttcgtg cggaacaccg
     8641 catcgtttct aacgcttcct gtaccacgaa ttgcataatt cccgtcatca aattgttaga
     8701 tgatgcgtac ggtattgagt ccggcactgt gaccacaatt cactccgcca tgcacgatca
     8761 acaggttatt gatgcatacc atccagacct gcgtcgcact cgggcagcca gtcagtcgat
     8821 cattccggtc gatactaaac tggccgccgg tatcacacga ttttttccgc aatttaacga
     8881 tcgctttgag gcgattgcgg tacgtgtgcc aaccataaat gtgacggcaa tcgatttaag
     8941 cgtgacggtg aagaaacctg taaaagccaa tgaagtcaac ctgttgctgc aaaaagcagc
     9001 acaaggtgca tttcatggta tagttgacta tacggaattg ccgttggtct ctgtagattt
     9061 taaccacgat ccgcacagtg ccattgtcga tggcactcaa acccgggtca gtggcgcaca
     9121 cctgatcaaa acgttggtct ggtgcgataa cgaatggggc tttgctaacc gaatgctcga
     9181 cacgacgtta gctatggcta ctgttgcttt caggtaagac gcaagcagcg tctgcaaaac
     9241 ttttagaatc aacgagagga ttcaccatgt ctgtaattaa gatgaccgat ctggatcttg
     9301 ctgggaaacg tgtatttatc cgtgcggatc tgaacgtacc agtaaaagac gggaaagtaa
     9361 ccagcgacgc gcgtatccgt gcttctctgc cgaccatcga actggccctg aaacaaggcg
     9421 caaaagtgat ggtaacttcc cacctgggtc gtcctaccga aggcgagtac aacgaagaat
     9481 tctctctgct gccggttgtt aactacctga aagacaaact gtctaacccg gttcgtctgg
     9541 ttaaagatta cctcgacggc gttgacgttg ctgaaggtga actggttgtt ctggaaaacg
     9601 ttcgcttcaa caaaggcgag aagaaagacg acgaaactct gtccaaaaaa tacgctgcac
     9661 tgtgtgacgt gttcgtaatg gacgcattcg gtactgctca ccgcgcgcag gcttctactc
     9721 acggtatcgg taaattcgct gacgttgcgt gcgcaggccc gctgctggca gctgaactgg
     9781 acgcgctggg taaagcactg aaagaacctg ctcgcccgat ggtggctatc gttggtggtt
     9841 ctaaagtatc taccaaactg accgttctgg actccctgtc taaaatcgct gaccagctga
     9901 ttgttggtgg tggtatcgct aacaccttta tcgcggcaca aggccacgat gtgggtaaat
     9961 ccctgtacga agctgatctg gttgacgaag caaaacgtct gctgaccacc tgcaacatcc
    10021 cggttccgtc tgatgttcgc gtagcaaccg agttctctga aactgcaccg gctaccctga
    10081 aatctgttaa cgatgtgaaa gctgacgagc agatcctgga tatcggtgat gcttccgctc
    10141 aggaactggc tgaaatcctg aagaatgcga aaaccattct gtggaacggt ccggttggcg
    10201 tgttcgaatt cccgaacttc cgcaaaggta ctgaaatcgt ggctaacgct atcgcagaca
    10261 gcgaagcgtt ctccatcgct ggcggcggcg acactctggc agcaatcgac ctgttcggca
    10321 ttgctgacaa aatctcctac atctccactg gcggcggcgc attcctcgaa ttcgtagaag
    10381 gtaaagtact gcctgcagta gcgatgctcg aagagcgcgc taagaagtaa aaaatcacag
    10441 ggcagggaaa cctgcccttg tttcagcgcg cttttagagc acgcactttt tcatactcta
    10501 aataattcga gttgcaggaa ggcgacaaga gagtgaatcc ccaggagctt acataagtaa
    10561 gtgattgggg tgagcgaacg tagacgcagc acatgcaact tgaagtatga cgagtataag
    10621 gcccgacgat acaggacaag agacatgtct aagatttttg atttcgtaaa acctggcgta
    10681 atcactggtg atgacgtaca gaaagttttc caggtagcaa aagaaaacaa cttcgcactg
    10741 ccagcagtaa actgcgtcgg tactgactcc atcaacgccg tactggaaac cgcagctaaa
    10801 gttaaagcgc cggttatcgt tcagttctcc aacggtggtg cttcttttat cgctggtaaa
    10861 ggcgtgaaat ctgacgttcc gcagggcgct gctatcctgg gtgcgatctc tggtgcgcat
    10921 cacgttcacc agatggctga acattatggt gttccggtta tcctgcacac cgaccactgc
    10981 gcgaagaaac tgctgccgtg gatcgacggt ctgctggacg cgggtgaaaa acacttcgct
    11041 gctaccggta agccgctgtt ctcttctcac atgattgacc tgtctgaaga atctctgcaa
    11101 gagaacattg aaatctgctc taaatacctg gagcgcatgt ccaaaatcgg catgactctg
    11161 gaaatcgaac tgggttgcac cggtggtgaa gaagacggcg tggacaacag ccacatggac
    11221 gcttctgcac tgtacaccca gccggaagac gttgattacg catacaccga gctgagcaaa
    11281 atcagcccgc gtttcaccat cgctgcgtcc ttcggtaacg tacacggtgt ttacaagccg
    11341 ggtaacgtgg ttctgactcc gaccatcctg cgcgactctc aggaatatgt ttctaagaaa
    11401 cacaacctgc cgcacaacag cctgaacttc gtcttccacg gtggttccgg ttctactgct
    11461 caggaaatca aagactccgt aagctacggc gtagtgaaaa tgaacatcga taccgatacc
    11521 caatgggcaa cctgggaagg tgttctgaac tactacaaag aaaacgaagc ttacctgcaa
    11581 ggccagctgg gtaacccgaa aggcgaagat cagccgaaca agaaatacta cgatccgcgc
    11641 gtatggctgc gtgccggtca gacttcgatg atcgctcgtc tggaaaaagc attccaggaa
    11701 ctgaacgcga tcgacgttct gtaagatatt cctttctgct tatctcaagg cccgctctgc
    11761 gggtcttttt ttcgccaaaa gcaaggtgat acagatgaga aatctgtgat ctatttggca
    11821 aaattatgct ttattgttta cccttgtcag actgcccgtc ataaggcggc ggagtgtatt
    11881 tctccatttt gagtcagttg aaaaggaata ttgaatggaa gatttgaatg ttgtcgatag
    11941 cataaacggc gcgggaagct ggctggtggc taaccaggcg ctgctgctaa gttatgcagt
    12001 aaacatcgtg gcggcactcg cgatcatcat cgttggtttg attatcgcgc ggatgatttc
    12061 caacgcggtg aatcgcctga tgatctcccg taaaatcgat gccactgttg ctgattttct
    12121 ttctgcatta gtccgttacg gaattatcgc ctttacgcta atcgcagcac tgggacgcgt
    12181 gggcgtacaa acagcgtcag tcattgctgt actcggtgcc gcaggtttag ctgttggtct
    12241 agctttgcag gggtcactgt ctaacctggc cgctggcgtg ttacttgtca tgttccgccc
    12301 gttccgtgcc ggagaatatg ttgacctcgg cggcgtagcc ggtactgtgc tgagtgtgca
    12361 gattttctcc accaccatgc gtactgcaga cggtaaaatt atcgttattc cgaacggtaa
    12421 aattattgcc gggaatatta ttaatttctc ccgcgagcca gctcgccgta acgaatttat
    12481 tattggcgtg gcgtatgatt ccgatatcga tcaggttaag cagatcctga ccgatattat
    12541 ccagtctgaa gatcgcattt tgaaagatcg cgaaatgact gtgcgtctga acgaacttgg
    12601 cgcatcgtcg attaatttcg tggtccgcgt ctggagcaac agcggcgatc tgcaaaacgt
    12661 gtactgggat gtgctggagc gtattaaacg tgaatttgat gccgccggta tcagcttccc
    12721 gtacccgcaa atggatgtga actttaagcg ggtgaaagaa gacaaagctg cgtaatcaac
    12781 gctgaggcca gataatactc tctggcctct ctcttattag tttttctgat tgccaattaa
    12841 tattatcaat ttccgctaat aacaatcccg cgatatagtc tctgcatcag atacttaatt
    12901 cggaatatcc aacgtgtttt cttattactt tcaaggtctt gcacttggcg cagctatgat
    12961 cctaccgctc ggtccacaaa atgcttttgt gatgaatcag ggcattcgtc gtcagtacca
    13021 cattatgatt gccttacttt gtgctatcag cgatttggtc ctgatttgtg cggggatttt
    13081 tggtggcagc gcgttattga tgcagtcgcc gtggttgctg gcgctggtca cctggggcgg
    13141 cgtagccttc ttgctgtggt atggttttgg cgcttttaaa acagcaatga gcagtaacat
    13201 tgagttagcc agtgccgaag tcctgaaaca aggcagatgg aaaattatcg ccaccatgtt
    13261 ggcagtgacc tggctgaatc cgcatgttta cctggatact tttgttgtgc tgggcagcct
    13321 tggcgggcaa cttgatgtgg aaccaaaacg ttggtttgcg ctcggaacaa ttagcgcctc
    13381 tttcctgtgg ttctttggtc tggctattct cgcagcctgg ctggcaccgc gcctgcgcac
    13441 ggcaaaatca cagcgcatta tcaatctggt agtgggatgt gttatgtggt ttattgcctt
    13501 gcagctggcg agagacggta ttgctcatgc acaagccttg ttcagttagg cgctatctga
    13561 tggaaaaata aaacagaggc gctaagcttg cctccagagg tcctgaattt tgggcaagtc
    13621 aattaacacg gagagactaa cgtgaagttc aaagttatcg ccctggcggc attaatgggt
    13681 attagcggga tggcagcgca ggctaacgaa ttgccggatg gaccgcatat tgtcacctcc
    13741 ggtacggcaa gcgtggatgc ggtgccagac attgccactc ttgcgattga agttaacgtg
    13801 gccgcgaagg atgccgctac tgccaagaaa caggcagatg agcgcgtcgc acaatacatt
    13861 tccttccttg agctcaatca gatcgcgaaa aaagatatca gctcagcgaa cttacgcact
    13921 cagccagatt atgattatca ggatggtaaa agtatcctga aaggctaccg cgcagtgaga
    13981 acggtggaag tcacgctccg tcagttagac aaactgaatt ccttgctgga cggcgcgcta
    14041 aaggcgggtc ttaacgaaat tcgttctgtg tcgctgggcg tggcgcagcc ggatgcctat
    14101 aaagacaaag cgcgtaaggc agcgattgat aacgcgattc atcaggcgca ggaactggcg
    14161 aatggcttta atcgcaaact ggggccggta tatagcgtgc gttatcatgt ttccaactat
    14221 cagcccagcc caatggtgcg gatgatgaaa gctgatgctg caccggtgtc cgcacaggaa
    14281 acttacgagc aggccgctat tcagtttgat gatcaggtcg atgtagtctt ccagttagaa
    14341 cctgtggatc aaccccccgc taaaacacct gctgcacatt aatacttgta gcccgacgta
    14401 ttcgtcgggc taatgtcttt acccgatatt gtctcctttt tctgtaaaac catgacgtaa
    14461 tcacctatct ttgctcgcgg tttcatcaag gataggctga ctgaaacggt taagcggaca
    14521 aggatgcggg agagggggga tggacatttt tatttcgaaa aagatgcgca atttcatttt
    14581 attagcgcaa accaataata ttgcccgggc ggcagaaaaa atccatatga cggcttcgcc
    14641 atttggcaaa agcattgccg cgctggaaga acaaattggc tatacgctat ttacccgcaa
    14701 agataataat atcagcctca ataaagccgg acaggaactg tatcaaaaac tgttcccggt
    14761 ttatcaacga ctttctgcga ttgataatga aatccataac tcaggacgtc gttcacgaga
    14821 aattgtgata ggcatagata atacttatcc gaccattatt tttgatcagt tgattagtct
    14881 cggagataag tacgaaggcg tgaccatcca gccagttgag ttcagcgaaa atggcgttat
    14941 cgataatctt tttgaccgtc agttagattt tataatctcc ccacagcatg tatctgcacg
    15001 agttcaggaa ctggaaaacc tgactatcag tgaactaccg ccgctgcgat tgggctttct
    15061 cgtttctcgc cgttatgaag agcgacaaga gcaagaactg cttcaggagc tgccctggct
    15121 acaaatgcgc tttcagaatc gggccaattt cgaagcaatg attgatgcga atatgcgtcc
    15181 ttgtgggatc aatcccacca ttatttatcg tccctacagt tttatggcta aaatcagcgc
    15241 cgtggagtgg ggacattttc tgacggtgat cccgcatttt gcctggcgac tggtgaatcc
    15301 ggcaacgctg aaatattttg atgcgccgca caggccgatg tatatgcagg aatatcttta
    15361 ttcaatcaga aatcatcgtt ataccgccac gatgcttcag catattgctg aagatcgtta
    15421 cgggacaaac cattaacccg gcatcgatcc ggttgcaatt aaattacggt gtaagtcgaa
    15481 gacgtggcta agatcgtggt gaatatgtcc gccggacgca ttttccagat agcgatgcag
    15541 ataatcccga cacataggat gtgcacaatg atcaatggtg gtgcggataa cgttgttata
    15601 cggatcctgt gcgccaaatc cactgaccgc ttcgctccat aaataacgtg ccgcacgcaa
    15661 catggcggcg ttcataaaca gatccatgcc aatgccgaag aaaaacgaca ggcgagggac
    15721 gaagccatca attttcagcc cggcggagat tgctgctttg atgtactcaa tcctatcggc
    15781 gagcgtaaat gctacctgct gcacgcagtt ggcacccgct tcacccatgt ggtaactgct
    15841 gatactgatg gtattaaatc gcggcatgtt gccggaacac caggcgatga tgtctgcgat
    15901 aatacgcatt gacggttttg gcgggtaaat ataggtattg cggcagaggt actctttgag
    15961 gatatcgttc tgaatggtgc cggtcagttt atcggatgta acaccttgct cttctgcggc
    16021 gacgatataa aacgccagta ctggtagcac tgcgccattc atggtcatcg aaaccgacat
    16081 tttatccagc gggatctggt cgaacaggac tttcatatct tccacggtgt cgatagcgac
    16141 gcccgctttg ccgacgtcgc ccgccacgcg tgggttatcg gagtcgtagc ctcggtgggc
    16201 ggccaggtta cggcgataaa aagcgttgga ctccttttgt tgtcgagaaa ccagcatact
    16261 gacggatggt ccacggttgg gtggtataca tagtggcacg cgggccacga acgtagggtg
    16321 gcaaaccagg aagagtaccc gtcacctcca gattatcgag atcggcttcg gtatacagcg
    16381 gcttgatagc gatcccttcc gcggtttgct gaaccagcga gtcgacagtt ttctcccgac
    16441 ggctcaattc cttgttggca agctgttgcc actcctgcac gttagacatt atttgctccg
    16501 taaaacgcaa tcaaaatgag ggtatttgcg cttacggtga gctttttacg tgggtttgtc
    16561 gccagcaaaa aaaagcattc agacgtcgtt ttttgccgcc tgagatttgt ctattttgtg
    16621 atgtgattca aggaatacaa aaagaagtga ggataacgcc tgatatgtgc tatcaggcgt
    16681 tatttgatgg attaatcctg acgaagaact ttgtgaccat aatcgagcaa cgcatcagtg
    16741 actttacgca tcatgcggct ttcaggagca aagcggtgcc agtagagcat ccggcgttgg
    16801 aatagccctg gcgttaagtc aatcagttcg ccgctggcca gctctttctc gatttgcagg
    16861 tgcgggatca tacagcaggt ggtgccctga cgagcaagtt gcacgaacgc ttccgaagaa
    16921 ttaacgatat ggcagggtac gctgccagga ggcagatcga aattttgctg caaaaaggcc
    16981 tggtgcatat cgtcaagatg gtcaaacgcg accactggcg ctttcagtaa tgccgaacgc
    17041 gttacgccgt tagggaaata tttttcggca aacggttttg agctgacgaa cagatagtcg
    17101 agcgcaccga gtttatcgac aagacaactc ggcagcgcct gatgttgaat actcactgcg
    17161 ccgaccactt cgccgcgacg caaacgttcc tgagtgcggg tttcatcttc tacctgcaag
    17221 ttgaggcgga tgggcgaatc agccaacaca ggagccagtg ccggaagcaa ccacgtcgcc
    17281 agactgtcgg cgttgaccgc cagtgaaagc agcagcggag tcgaaccggt ttgttcatcg
    17341 cccagccact cttcttccag cagctccacc tggcgtagta gcgccaacag tttttgccct
    17401 tgttccgtcg ggcgcggcgg tacggtacgc actaacaacg gctgcccgaa catattttcc
    17461 agttgcttaa tgcgctgtga gacggctgat tgtgtaatgc acagcttttg tgccgcgcgc
    17521 tcaaatcctc gttcacgtat caccgcatcc agtgcctgta atgttctgta gtccgggcgt
    17581 ttcattgctg ttgtctgctc ctggtaaatg ctttttcctg cactatgaca caattttgtg
    17641 tcgggttgca tataccgaag ccgttggcgg gtaacatttg tatcagtggt taacgcgcag
    17701 cttaaaagat cagggatttg cgctaatgca tttcgctcag cgagttcgcg ccctggttgt
    17761 gttgaatggc gtggcgttat tgcctcagtt tgcctgtaaa caggggcttg cgaacgggga
    17821 actggtgcgc ctgtttgcac cgtggagcgg catccccaga ccgttgtatg ctttatttgc
    17881 ggggcgaaag gggatgcccg ccattgcgcg atattttatg gatgagttaa ccacgcggct
    17941 tgccaacggg gtctgaatcg ctttttttgt atataatgcg tgtgaaattt cataccacag
    18001 gcgaaacgat catgacgcag gatgaattga aaaaagcagt aggatgggcg gcacttcagt
    18061 atgttcagcc cggcaccatt gttggtgtag gtacaggttc caccgccgca cactttattg
    18121 acgcactcgg tacaatgaaa ggccagattg aaggggccgt ttccagttca gatgcttcca
    18181 ctgaaaaact gaaaagcctc ggcattcacg tttttgatct caacgaagtc gacagccttg
    18241 gcatctacgt tgatggcgca gatgaaatca acggccacat gcaaatgatc aaaggcggcg
    18301 gcgcggcgct gacccgtgaa aaaatcattg cttcggttgc ggaaaaattt atctgtattg
    18361 cagacgcttc caagcaggtt gatattctgg gcaaattccc gctgccagta gaagttatcc
    18421 cgatggcacg tagtgcagtg gcgcgccagc tggtgaaact gggcggtcgt ccggaatacc
    18481 gtcagggcgt agtgaccgat aacggcaacg tgatcctcga cgtccacggc atggaaatcc
    18541 ttgacccgat agcgatggaa aacgccataa atgcgattcc tggcgtggtg actgttggtt
    18601 tgtttgctaa ccgtggcgcg gacgttgcgc tgattggcac acctgacggt gtcaaaacta
    18661 ttgtgaaatg atctgacggg ggaacctccc ccgttaaaaa aattctattc attaaatttg
    18721 gtgacatgtg tcacgttttt acctggcaat tgtcgattgc tctaaataaa tcctctaaac
    18781 cagcatattc atccaagaaa tacttttgcg tgatatttcc tcaacatcgc gacgcaaacg
    18841 ttcatattgc cgcaatatta ttttttgata tgttgaaagg cggatgcaaa tccgcacaca
    18901 acatttcaaa agacaggatt gggtaaatgg caaaggtatc gctggagaaa gacaagatta
    18961 agtttctgct ggtagaaggc gtgcaccaaa aggcgctgga aagccttcgt gcagctggtt
    19021 acaccaacat cgaatttcac aaaggcgcgc tggatgatga acaattaaaa gaatccatcc
    19081 gcgatgccca cttcatcggc ctgcgatccc gtacccatct gactgaagac gtgatcaacg
    19141 ccgcagaaaa actggtcgct attggctgtt tctgtatcgg aacaaaccag gttgatctgg
    19201 atgcggcggc aaagcgcggg atcccggtat ttaacgcacc gttctcaaat acgcgctctg
    19261 ttgcggagct ggtgattggc gaactgttgc tgctattgcg cggcgtgccg gaagccaatg
    19321 ctaaagcgca tcgtggcgtg tggaacaaac tggcggcggg ttcttttgaa gcgcgcggca
    19381 aaaagctggg tatcatcggc tacggtcata ttggtacgca attgggcatt ctggctgaat
    19441 cgctgggaat gtatgtttac ttttatgata ttgaaaacaa actgccgctg ggcaacgcca
    19501 ctcaggtaca gcatctttct gacctgctga atatgagcga tgtggtgagt ctgcatgtac
    19561 cagagaatcc gtccaccaaa aatatgatgg gcgcgaaaga gatttcgcta atgaagcccg
    19621 gctcgctgct gattaatgct tcgcgcggta ctgtggtgga tattccagcg ctgtgtgacg
    19681 cgctggcgag caaacatctg gcgggggcgg caatcgacgt attcccgacg gaaccggcga
    19741 ccaatagcga tccatttacc tctccgctgt gtgaattcga caatgtcctt ctgacgccac
    19801 acattggtgg ttcgactcag gaagcgcagg agaatatcgg tctggaagtt gcgggtaaat
    19861 tgatcaagta ttctgataat ggttcaacgc tctctgcggt gaacttcccg gaagtctcgc
    19921 tgccactgca cggtggtcgt cgtctgatgc acatccacga aaaccgtccg ggagtgctga
    19981 ctgcgctcaa caaaattttt gccgagcagg gcgtcaacat cgccgcgcaa tatctgcaaa
    20041 cttccgccca gatgggttat gtagttattg atattgaagc cgacgaagac gttgccgaaa
    20101 aagcgctgca ggcaatgaaa gctattccgg gtaccattcg cgctcgtctg ctgtactaaa
    20161 tccccttctc tgaaaatcaa cgggcaggtc actgacttgc ccgttttttt atcccttctc
    20221 cacaccgttg ccgcattgcg cattctactt tgcgagtctg ctccagaaaa taaatcaaag
    20281 tcagatcaat gcgttgtgtt tatttaatgg ggatttacga tggcgggact gcacagggct
    20341 gtaacggtaa agccctcacc gaagcgaggg cttgaaggag aagggttatg atgcgacttg
    20401 tcatcatact gattgtactg ttactcataa gtttcccagc ttattaacag tcagtctcag
    20461 gggaggagtc atcctccctt acccttactc actaaattag gttaaagaat caacgatgtc
    20521 aatcagggcg atgcaggtgt gtcgccctca ccactcccag actttcgacg gtgtaaccac
    20581 cgcaggaaga gggatatccc actcttcaac ggggagtttt tccaccaact gacaatcatg
    20641 cgcataaccc accggttgca ttttatagtg ctgccagttt tgtaaggtcc gatcataaaa
    20701 accaccgccc attcccaagc gttgaccgta ttcatcaaaa gccaccagcg gcgtgatcaa
    20761 tacatctaac cgggaaaggg gaagcacatc gcgcacgtcc agttttggct cgtggatctt
    20821 caacctgttc atcaccagtt cgctttgcgg atggtaattc aggaacagca agttaccggc
    20881 actaaaggga tgcaaaactg gaagatatac gcgcttaccg gcgcgccaga gttgttctat
    20941 gagtggctgg gtgtcgagtt cgccatcaaa agagaggaat acagcgaccg tatgcgccat
    21001 caccactggg ggatacgtca tcatccgggt agcggcttgt tgacccattt cctgctgttg
    21061 ttccggcgtt aacgtacgac gacgttgccg aatcattttg cggatttctt gtcgggataa
    21121 tgtcagtggg agttctggta gttgcgtcat ggctggtgcc aggtaagaag ggaatctccg
    21181 agatgccgcc gcaggctgta acccttgaac ccttggttca aggtgaatgt gtcgtcgcag
    21241 ttttaaggct tctcggacgg accgagcatg ctcaccaacc gcggagcgcc acattcttgt
    21301 ggtatgaaat atcggctcag gggactggcc cgcttgcgaa catctcagag aaattttgtc
    21361 ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt caaagttttg gttagttttt
    21421 tcggtgatgc gaccttgttc aagtaacgct tgttctatgg tctgctgcag catccgaata
    21481 cgctgttcca tacttgctgc gtagtcacga gtcttcgctt tttcttgcgc taactcatag
    21541 ctgatattca atgcggcaat gaagaccaac tgttcagtat ttgtgactct agtgcgttct
    21601 ttcagatctt gcaaccgttg gttcagatcg tccgctgcct gattcaacgc atccctttgg
    21661 tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt ggatatcgac gggttgtgca
    21721 gacatgccac cttcctgctg attgactgcg ctgccttcgt ctttagaccc atggtctgcg
    21781 aaggggcgac actatagcta ccctgatgag aagagacaag cccttttctg gtccaccagg
    21841 gcccaaagtg gtagcatatc atgaatattc ctccctttga cgacgaatgc ttatgtctat
    21901 acagaacgaa atgcctggtt acaacgaaat gaaccagtat ctgaaccaac aagggacggg
    21961 tctgacccct gctgagatgc atggtttaat cagtgggatg atatgtggcg gtaacgatga
    22021 cagctcatgg ctgccgctac ttcacgacct gacgaacgaa ggcatggctt tcggtcatga
    22081 gctggcacag gcactgcgca aaatgcactc tgccaccagc gatgccctgc aggatgacgg
    22141 cttccttttt cagctttatc tgcctgatgg cgatgatgtc agcgttttcg atcgggctga
    22201 tgcgctggct ggttgggtca atcacttcct gcttggtctt ggcgttacgc aaccgaagct
    22261 ggacaaagtg accggcgaaa ccggtgaagc catcgacgat ctgcgtaaca tcgcacagtt
    22321 gggttacgac gaagacgaag atcaggaaga gcttgaaatg tcgcttgaag agatcatcga
    22381 gtacgtccgt gttgccgcgc tgttatgcca cgacaccttt actcatccgc aaccgaccgc
    22441 gccagaagta caaaaaccga ctctacacta aaaacaaaaa acgtaaggag agtgttatga
    22501 gtgatatatc ccggcaagaa tttcagcgtc gccgtcaggc cctggtagag caaatgcaac
    22561 ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac acgtagcgcc gacagcgaat
    22621 acccctatcg tcagaacagt gacttctggt acttcaccgg ctttaacgaa ccggaagcgg
    22681 tgctggtgct gattaaaagc gatgacactc ataaccacag cgttttgttt aaccgcgttc
    22741 gcgacctgac ggcagagatc tggtttggtc gtcgcttagg ccaggatgcc gcgccagaga
    22801 aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa tcagcaactt tatcaactac
    22861 ttaatggtct ggatgtggtt taccacgctc agggcgaata tgcatatgct gatgaaatcg
    22921 tgaacagcgc gcttgagaaa ctgcgtaaag gttcgcggca aaatctcact gcaccggcaa
    22981 cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct gttcaaatca ccagaagaga
    23041 ttgccgtact tcgccgcgcg ggtgaaatca ccgcactggc gcatacacgg gcgatggaaa
    23101 aatgccgtcc ggggatgttc gagtaccatc tggaaggcga aattcaccac gaatttaacc
    23161 gccacggtgc gcgttaccct tcctataaca ccattgttgg cagcggtgaa aacggctgca
    23221 ttttgcacta caccgaaaac gagtgtgaac tgcgtgacgg tgacctggtg ttgattgacg
    23281 cgggttgtga atacaaaggt tacgctggcg acattacccg caccttcccg gtcaacggca
    23341 aattcactca ggctcagcgt gaaatctatg acattgtgct ggagtctctt gaaaccagcc
    23401 tgcgcctgta tcgtccggga acttccattc aggaagtcac tggtgaagtg gtgcgcatca
    23461 tggttagcgg tctggtaaaa ctcggcatcc tgaaaggtga tgttgatgaa cttatcgctc
    23521 agaacgccca tcgtcctttc tttatgcatg gccttagcca ctggttaggg ctggatgttc
    23581 atgacgttgg tgtttatggt caggatcgct cgcgtattct ggaaccgggt atggtactga
    23641 ccgtagaacc agggctgtat attgcgccgg atgcagaagt gccagaacag tatcgcggta
    23701 tcggcattcg tattgaagac gatattgtga ttaccgaaac cggtaatgaa aacctcaccg
    23761 ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat ggcagctgcg agaaagcaat
    23821 gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg ctggcgctgg ctatttcccg
    23881 gttaagtcac ggggcgctgc cagtacattt gattgaagcg accgcgccag agtcacatgc
    23941 gcatccgggc tttgatggac gagctattgc gctggcggcg ggtacctgtc agcaactggc
    24001 gcgcatcggc gtctggcaat ctctggctga ttgcgcaact gccatcacca cagtgcatgt
    24061 cagcgatcgt ggtcacgctg gatttgtcac cctcgccgca gaagattacc aactggcggc
    24121 gctggggcag gttgtcgaat tgcacaatgt cgggcaacgg ctgtttgcat tgctgcgcaa
    24181 agcacctggc gtaacgctgc attgccctga tcgcgtggct aacgttgccc gtactcagag
    24241 ccacgttgaa gtgacgctgg agagtggtga gacgctgacg ggccgcgtgc tggtagccgc
    24301 tgatggcacc cattcagcgt tagccaccgt gtgcggcgtt gactggcagc aggatcctta
    24361 cgaacaactg gctgtgattg cgaacgttgc gacttccgtt gcgcatgaag ggcgcgcttt
    24421 tgaacgcttt acgcaacatg gcccgctggc gatgttgccg atgtctgacg gacgctgttc
    24481 gctggtctgg tgtcatccac tggagcggcg cgaagaggtg ctgtcgtgga gcgacgagaa
    24541 gttttgccgt gaactccagt cggcctttgg ctggcgactt gggaaaatta ctcacgccgg
    24601 taaacgcagt gcttatccgc tggcgttaac ccgcgccgcc aaacctatta cccatcgtac
    24661 cgtgctggtg ggcaatgcgg cgcaaactct gcaccccatc gccgggcaag gatttaacct
    24721 cggtatgcgt gatgtcatga gccttgcgga aaccctgact caggcgcagg aacgcggaga
    24781 agacatgggt gattacggcg tattgtgccg ttatcagcag cgtcgacaga gcgatcgcga
    24841 agcaaccatc ggcgtcacgg acagccttgt tcatcttttt gccaaccgtt ggacgccgct
    24901 ggttgtcggg cgcaacatcg ggctgatgac gatggaatta tttaccccgg cacgcgatgt
    24961 gctggcgcag cgcactcttg gttgggtggc gcgttgaagg cggttctaag gaataaacaa
    25021 tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt cgggctggcg gttgcctgcg
    25081 gcttacaggg gagcggctta cgcgttgccg tactggagca gcgcgcaccg gaacctctgg
    25141 cggcggatgc accaccacaa ctgcgcgttt cggctatcaa tgccgccagc gaaaaattac
    25201 tcacccgtct tggcgtctgg caggacattc tctctcgtag ggcctgttgc tatcacggta
    25261 tggaagtgtg ggataaagac agttttggtc acatctcgtt tgacgatcaa agcatgggct
    25321 atagccatct tgggcatatc gttgaaaatt cagtgattca ctacgcgctg tggaacaaag
    25381 cgcagcagtc gtcagatatc actctgttgg cccccgcaga attacagcag gtcgcctggg
    25441 gagaaaatga aaccttcctg acgttgaaag atggcagcat gttaacggcg cgtctggtga
    25501 ttggcgcgga cggcgctaat tcctggttgc gcaacaaagc cgatattccg ctgactttct
    25561 gggattatca gcatcacgcg ctggtggcga ccattcgcac ggaagaaccg catgatgcgg
    25621 tggcgcggca ggttttccat ggcgaaggca ttctggcctt tttaccgctt agcgatccgc
    25681 atctttgctc gattgtctgg tcactgtcgc cagaggaagc gcagcggatg cagcaggcag
    25741 gggaagacga atttaatcgc gcgttaaata tcgcttttga taatcgcctg ggcttatgca
    25801 aggttgagag tgagcgtcag gtgttcccgc tgacggggcg ttatgcgcgc cagtttgccg
    25861 cgcaccgtct ggtgttggtg ggcgacgccg cgcataccat tcacccgctg gcggggcagg
    25921 gggtaaatct cggctttatg gatgctgcag agctgattgc tgaactgaaa cggttgcatc
    25981 gtcagggtaa agacatcggg cagtacattt acctgcgtcg ctatgagcgt agccgcaagc
    26041 acagcgcggc gctgatgctg gctggtatgc agggattccg cgatctgttt tccggggcta
    26101 atccggcgaa aaaattgctg cgtgatattg gtctgaaact ggctgatacg cttcctggcg
    26161 ttaagccaca acttattcgt caggcaatgg gattaaacga tttgcctgaa tggctgcgtt
    26221 aaatatttct cttctgctgt ttatttgata cccatcacac tttcatctcc cggttttttc
    26281 gccgggggat tttcctcatt tgaaataaac taatttaacc tccgttttcg cattatattt
    26341 tctaatgtta tggatttttg atttagtgtt ttttgacatt tttttagcgc ttaatattgt
    26401 cttattcaaa ttaactttct catcacatca tctttgtata gaaactgatg tgttttttgg
    26461 ttttttattc tgttgcgatt ttggcatttt ttaaccataa gctaatgtga tgatcaattt
    26521 taccttatgg ttaacagtct gtttcggtgg taagttcagg caaaagagaa cgattgcgtt
    26581 ggggaccggg agtggctccg atgctgggtt tcgtggtgat aatttcgcca tggaaaagtt
    26641 gtcagccccg cttattcaat gaggacaaga tggcacaaca gactcctttg tacgaacaac
    26701 acacgctttg cggcgctcgc atggtggatt tccacggctg gatgatgccg ctgcattacg
    26761 gttcgcaaat cgacgaacat catgcggtac gtaccgatgc cggaatgttt gatgtgtcac
    26821 atatgaccat cgtcgatctt cgcggcagcc gcacccggga gtttctacgt tatctgctgg
    26881 cgaacgatgt ggcgaagctc accaaaagcg gcaaagccct ttactcgggg atgttgaatg
    26941 cctctggcgg tgtgatagat gatctcatcg tctactactt tactgaagat ttcttccgcc
    27001 tcgttgttaa ctccgccacc cgcgaaaaag acctctcctg gattacccaa cacgctgaac
    27061 ctttcggcat cgaaattact gttcgtgatg acctttccat gatcgccgta caagggccga
    27121 atgcgcaggc aaaagctgcc acactgttta atgacgccca gcgccaggcg gtggaaggga
    27181 tgaaaccgtt ctttggcgtg caggcgggcg atctgtttat tgcaaccacc ggttacaccg
    27241 gtgaagcggg ctacgaaatt gcgctgccca atgaaaaagc ggccgatttc tggcgcgcgc
    27301 tggtggaagc tggcgttaag ccatgtggcc tgggcgcgcg tgacacgctg cgtctggaag
    27361 cgggcatgaa tttgtatagc caggagatgg acgaaactat ttctccttta gccgccaata
    27421 tgggctggac tatcgcctgg gaaccggcag atcgtgactt tatcggtcgt gaagctctgg
    27481 aagcgcagcg tgaacatggc acagaaaaac tggttggtct ggtgatgacc gaaaaaggcg
    27541 tgctgcgtaa tgaactgccg gtacgtttta ctgatgcgca gggcaaccag catgaaggca
    27601 ttatcaccag cggtactttc tccccaacgc tgggttatag cattgcgctg gcgcgcgtgc
    27661 cggaaggtat tggtgaaacg gcgattgtgc aaattcgcaa ccgtgaaatg ccggttaaag
    27721 tgacgaaacc tgtttttgtg cgtaacggca aagccgtcgc gtgatttact tttttggaga
    27781 ttgattgatg agcaacgtac cagcagaact gaaatacagc aaagaacacg aatggctgcg
    27841 taaagaagcc gacggcactt acaccgttgg catcaccgaa cacgctcagg agctgttagg
    27901 cgatatggtg tttgttgacc taccggaagt gggcgcaacg gttagcgcgg gcgatgactg
    27961 cgcggttgcc gaatcggtaa aagcggcgtc agacatttat gcgccagtaa gcggtgaaat
    28021 cgtggcggta aacgatgcac tgagcgattc tccggaactg gtgaacagcg aaccgtatgc
    28081 aggcggctgg attttcaaaa tcaaagccag cgatgaaagc gaactggaat cactgctgga
    28141 tgcaaccgca tacgaagcat tgttagaaga cgagtaacgg ctttattcct cttctgcggg
    28201 agaggatcag ggtgaggaaa tttacgcctc accctcactc tcttcgtaag gagagaggtt
    28261 cacaattcac tgcacgtttc aggaaccatc gctcatgaca cagacgttaa gccagcttga
    28321 aaacagcggc gcttttattg aacgccatat cggaccggac gccgcgcaac agcaagaaat
    28381 gctgaatgcc gttggtgcac aatcgttaaa cgcgctgacc ggccagattg tgccgaaaga
    28441 tattcagctt gcgacaccac cgcaggttgg cgcaccggcg accgaatacg ccgcactggc
    28501 agaactcaag gctattgcca gtcgcaataa acgcttcacg tcttacatcg gcatgggtta
    28561 caccgccgtg cagctaccgc cggttatcct gcgtaacatg ctggaaaatc cgggctggta
    28621 taccgcgtat actccgtatc aacctgaagt ctcccagggc cgccttgaag cactgctcaa
    28681 cttccagcag gtaacgctgg atttaaccgg gctggatatg gcctctgctt ctcttcttga
    28741 cgaagccacc gctgccgccg aagcaatggc gatggcgaaa cgcgtcagca aactgaaaaa
    28801 tgccaaccgc ttctttgttg cttctgacgt tcatccgcaa acgctggatg tggtccgtac
    28861 tcgtgccgaa acctttggtt ttgaagtgat tgtcgatgac gcgcaaaaag tgctcgacca
    28921 tcaggacgtc ttcggcgtgc tgttacagca ggtcggcact actggtgaaa ttcacgacta
    28981 cactgcgctt atcagcgaac tgaaatcacg caaaattgtg gtcagcgttg ccgccgatat
    29041 tatggcgctg gtgctgttaa ctgcgccagg taaacagggc gcggatattg tttttggttc
    29101 ggcgcaacgc ttcggcgtgc cgatgggcta tggtggccca cacgcggcat tctttgcggc
    29161 gaaagatgaa tacaaacgct caatgccggg ccgtattatc ggtgtatcga aagatgcagc
    29221 tggcaacacc gctctgcgca tggcgatgca gactcgcgag caacatattc gtcgtgagaa
    29281 agcgaactcc aacatttgta cttcccaggt actgctggca aacatcgcca gcctgtatgc
    29341 cgtttatcac ggcccggttg gcctgaaacg tatcgctaac cgcatccacc gtctgaccga
    29401 tatcctggcg gcgggcctgc aacaaaaagg tctgaagctg cgccatgcgc actatttcga
    29461 tactttgtgt gtggaagtgg ccgacaaagc gggcgtgctg gcgcgtgccg aagcggctga
    29521 aatcaacctg cgtagcgata ttctgaacgc ggttgggatc acccttgatg aaacaaccac
    29581 gcgcgaaaac gtgatgcagc ttttcagcgt gctgttgggc gataaccacg ggctggacat
    29641 cgacacactg gacaaagacg tggctcacga cagtcgctct attcaggctg cgatgctgcg
    29701 cgacgacgaa atcctcaccc atccggtgtt taatcgctac cacagcgaaa ccgaaatgat
    29761 gcgctatatg cactcgctgg agcgtaaaga tctggcgctg aatcaggcga tgatcccgct
    29821 gggttcctgc actatgaaac tgaacgccgc cgccgagatg atcccgatca cctggccgga
    29881 atttgccgaa ctgcacccgt tctgcccgcc ggaacaggct gaaggttatc agcagatgat
    29941 cgcgcagctg gctgactggc tggtgaaact gaccggttac gacgccgttt gtatgcagcc
    30001 gaactctggc gcacagggcg aatacgcggg cctgctggcg attcgtcatt atcatgaaag
    30061 ccgcaacgaa gggcatcgcg atatctgcct gatcccggcc tctgctcacg gcactaaccc
    30121 ggcttccgcg catatggcag gaatgcaggt ggtggttgtg gcgtgtgata aaaacggcaa
    30181 catcgatctg actgatctgc gcgcgaaagc cgaacaggcg ggcgataacc tctcctgtat
    30241 catggtgacc tatccttcca cccacggcgt gtatgaagaa acgatccgtg aagtgtgtga
    30301 agtcgtgcat cagttcggcg gtcaggttta ccttgatggc gcgaacatga acgctcaggt
    30361 tggcatcacc tcgccgggct ttattggtgc ggacgtttcc caccttaacc tgcataaaac
    30421 tttctgcatt ccgcatggcg gtggtggtcc gggtatggga ccgatcggtg tgaaagcgca
    30481 tctggctccg tttgtaccgg gtcatagcgt ggtgcaaatc gaaggcatgt taacccgtca
    30541 gggcgcggtt tctgcggcac cgttcggtag tgcctctatc ctgccaatca gctggatgta
    30601 catccgcatg atgggcgcag aagggctgaa aaaagcaagc caggtggcaa ttcttaacgc
    30661 caactatatt gccagccgtc tgcaggatgc cttcccggtg ctgtataccg gtcgcgacgg
    30721 tcgcgtggcg cacgaatgta ttctcgatat tcgcccgctg aaagaagaaa ccggcatcag
    30781 cgagctggat attgccaagc gcctgatcga ctacggtttc cacgcgccga ccatgtcgtt
    30841 cccggtggcg ggtacgctga tggttgaacc gactgaatct gaaagcaaag tggaactgga
    30901 tcgctttatc gatgcgatgc tggctatccg cgcagaaatt gaccaggtga aagccggtgt
    30961 ctggccgctg gaagataacc cgctggtgaa tgcgccgcac attcaaagcg aactggtcgc
    31021 cgagtgggcg catccgtaca gccgtgaagt tgcagtattc ccggcaggtg tggcagacaa
    31081 atactggccg accgtgaaac gtctggatga tgtttacggc gaccgtaacc tgttctgctc
    31141 ctgcgtaccg attagcgaat accagtaatt tactgattcg actatcttat aaaggcgctt
    31201 cggcgccttt ttcgcttgtt aataaagtgc gctttgtgtg tttcggctga ggcgtaatat
    31261 aaaatattgc gagctcaata tattataaga gtgccaggcc tgatgagcgg agcacatcag
    31321 gtacatcggc taattacaga ttctcgccgt tgctggcaat cacctctttg taccagttaa
    31381 agctcttctt acgtgaacgc gacatatcgc cagtaccgtc gtcatgttta ttcacataga
    31441 taaagccgta gcgtttgctg tactgcccgg tggtgaacga cacacagtcg atgcagcccc
    31501 acggcgtgta gcccatcagg tccacgccat cgtaagtcac cgcttttttc atctcttcaa
    31561 tatgggcacg caggtaatca atgcggtaat cgtcattgat gctgccatca tcttccactt
    31621 tgtcgtaagc gccaaaaccg ttttcgacaa taaacagcgg cttctggtaa cgctcataca
    31681 gctcgcaaag tgcgtagcgc agaccaaccg gatcaatctg ccagccccag tcagacgctt
    31741 taacatacgg attcggtacg ctgccttcga aaccagaaat cgcatcgccg gtgccacctt
    31801 cggccttcac cgcgttggtc atgtaatagc tgaagccgag ataatcgcag gtgccttcgc
    31861 gcagtacatc cagatcgccg tcttccattt tgatgttaaa tccgcggcgt tcccactcgt
    31921 tcaacacata ggacgggtaa tagccacgca actgcacatc ggtaaagacg taacgttcgc
    31981 gcatcgactc ctgggcaaac atcacatcgt ccgggttaca ggcgtaggga tagagcggca
    32041 ccatcgccag catacaaccg actttcatct ccgggttgat gcgatgcgcg gctttcaccg
    32101 ccagggcgct ggcgacaaac tggtgatgca gcacctgata catggtctct tccgggtttt
    32161 catgttcggt atacaccacg ccggagcagc agtaaccgaa cagcggtgca cgccagttac
    32221 gctggttgtt aatttcgttg aaggtcatcc agtatttgac tttgtgctta tagcgttcga
    32281 ataccacttc cgcgaaacgg acaaagaaat caaccacttt acggttggtc cagctaccgt
    32341 attgctgcac cagatgcagc ggcatttcaa agtgggagag ggtgatcacc ggttcgatgt
    32401 tgtatttcag cagttcatcg atcatatcat cgtagaactt cagcccttct tcgtttggct
    32461 gagcttcatc gccttttgga aaaatgcgcg tccaggcgat ggaagtacga aaacatttga
    32521 agcccatttc ggcaaatagc ttgatgtcct ccttgtagtg accataaaaa tcaacggctt
    32581 catggtttgg atagtatttg ccaggcacaa cttctttggt gatttcgcgc ggcacgccgt
    32641 gtgcgccacc ggtcaaaacg tcacaaatgc tcggcccttt gccgcctttg ttccagccgc
    32701 cttcgacctg atgagcggca actgcgccgc cccataagaa atctttcggt aaggtgagtt
    32761 ttttcactat cataggctcc agatgagtca attcttacag gaaatattac catgcagcca
    32821 aacgacatca ctttttttca acgtttccag gatgacattc tggctgggcg taaaaccatc
    32881 accatccgcg acgagtttga atcgcacttc aaaacgggtg atgtgcttcg tgtcggacgt
    32941 tttgaagatg acggttattt ttgcacgatt gaagtcaccg caacctcaac cgtaacgctg
    33001 gatacgctga cggaaaaaca tgcagagcag gaaaatatga ccctgactga actgaaaaag
    33061 gtcattgccg acatctatcc cgaccagaca cagttttatg tgattgaatt taaatgtctt
    33121 taaattgaac tcacgcctgt tagttgaaat ttcgcattta atttcatgat ttttcggaga
    33181 ttaatctcaa atcagaattt attttagcta acaggtgttc actggaacta ttctcagtta
    33241 cgctggagag gtatacatgt gcgtgtacgt ttttccggag taagttatgg ttcagaagcc
    33301 cctcattaag cagggatatt cgctggcaga ggaaattgcc aacagcgtca gtcacggcat
    33361 tgggctggtg tttggtatcg ttgggctggt gttgctactg gttcaggcgg tggatctcaa
    33421 tgccagcgcc acggcgataa ccagttacag cctctatggc ggcagtatga tcctgctgtt
    33481 cctcgcttcg acgctctatc acgccattcc ccatcaacgg gcaaaaatgt ggctgaagaa
    33541 atttgaccac tgtgccattt atctgttgat tgccggaacc tacacgccgt ttttgctggt
    33601 ggggctggat tctccgttag cgcgcgggtt gatgattgtt atctggagcc tggcattgct
    33661 gggtattctg tttaaactga ccatcgcgca ccgattcaaa attttatctc tggtgaccta
    33721 tctagcgatg ggctggctgt cgctggtggt aatttatgaa atggcagtta agctcgcggc
    33781 gggcagcgtt accttactgg cggtaggcgg tgtggtttat tcgctcgggg tgattttcta
    33841 cgtctgcaag cgcattccat acaatcatgc catctggcac ggctttgtgc tcggcggtag
    33901 tgtgtgccac tttctggcga tctatttgta tattgggcag gcgtaattgg cgtgcctttg
    33961 taggcctgaa aagacacacc agtgtcgcat caggcgataa atgcctgatg cgatacagag
    34021 atttactctt ccagcgaata tggcagtggc tcgatacgca atgtattcgc atcgtcacac
    34081 acgcggaaga tgctgtccgg ttccatatcg ttattcatga caacctgcac cacaacctga
    34141 ccatcttcca gtttgaccgc agccagcacc gtaccggtac gacgccagtt ctcgcccatt
    34201 ttcagctcta agtcttcacc agcttccggc agtcggctgg cgctaccctt cagcaaccag
    34261 agcgcgcgtt tgttggcacc acggaatttt gctcgcgcca ccatctcttg tccggtatag
    34321 cagcctttct taaagctgat accgcccagc gcctggaggt tggtcgcctg ggggataaac
    34381 tgcccgctgt tggcggcatc aatcaccgga aaacccgctt caatgtttaa tgccagccac
    34441 tgttggctat tgttcagttc cgcttcaccg cgcagtttat cggtcagcat attggcagta
    34501 gcttcatcgg ttacgatcag gaaacgttct gccgggtgtt caaaccatag caacgtggtc
    34561 gcgccttctt tgactacctg tttttctctc gaaggtagtt cactaaagag atttgccaac
    34621 gcggcgcgcg cctgaaaacc ggcaacacca agcagcacac gctcgtcgtc tggcgcgatg
    34681 gtcactttag agaacaccgc atattttttc agttcagtca gctgtggttc acgcacgctg
    34741 cgccgttcaa tccatgcaaa gccatcgccg tcgcggaaca gacgtaaatt gctccacatt
    34801 ttacctttgg cgtcgcaatg ggcggcgagc aggtgctgat attcagtcat ctggctgaca
    34861 tctgctgtca cctggccttg catatatttt tcgctgtccg cgccagtaat agtggcaagc
    34921 gcccagtcat caagcgtcat cagcgtcagt ggcaaacggg cagaagccgt aggctgacgg
    34981 ggaggaaaag gtgtaaaagc cataatagag tcctgagttg cttaacggcg tattaataac
    35041 taatggtaaa agagctatga ggcaatgcaa gtgctttcac tacgcgatct gtgccttctg
    35101 cgaggcgact tccaaaaaaa tgaagccctt gaattgttgt gaattaattt cgcgatcgcg
    35161 attgtgttta ctgatattcc cgtaaaagaa gtcgaaaaac acgttacaat agccgggtac
    35221 tggattttcg tgagacagga agaagatgga cattaacaac aaagcccgca ttcattgggc
    35281 atgccgccgt ggtatgcgcg aactcgatat ttcaatcatg ccgtttttcg aacatgagta
    35341 cgacagctta agcgatgacg aaaaacgcat ctttattcgt ctgctggaat gtgacgatcc
    35401 ggacctgttt aactggctga tgaatcacgg taaaccggcc gatgcagaac tggaaatgat
    35461 ggtccgactc atccagacac ggaaccggga acgtggtcct gtggcaatct gatttgcgcg
    35521 tctcctggcg cgcacagtgg ctttccttgc tgattcatgg gctggttgcc gctgttattt
    35581 tactcatgcc ctggccgctc agttacaccc cgttatggat ggtgttactt tcgctggtgg
    35641 tgtttgattg cgttcgcagc cagcggcgca ttaatgctcg ccagggggaa attcgcttgt
    35701 tgatggacgg gcgtttgcgt tggcaagggc aggagtggag catcgtcaaa gcgccgtgga
    35761 tgattaagag cggcatgatg ctgtgtttac gttctgacag cggaaaacgg caacatttat
    35821 ggctggcagc tgacagcatg gacgaagccg aatggcggga tttacggcgg atattgttac
    35881 aacaagagac gcaaagataa agatgacggc aaagcatatg tgctaagccg tcagtgacat
    35941 caggcgtaat gctctgccat ttcgttgagg atttgctcgc accagctctg aatacgctcg
    36001 tcgctaaggt catactggtt agtttcatcc agcgccagac ccacgaacag ttgcccgtca
    36061 gcaatcaccg gtttcgggct ggtaaattca tatccttccg ttggccagta gccgacgaac
    36121 ttcacgcctt tggtcgagag tttgtcatgc agcataccga gcgcatcgag gaaccactcg
    36181 ccgtatccca gttgatcgcc aagcccatac agcgcaacaa ttttaccttc gaggttcagg
    36241 tcgtcgagct gatcccagac ggcttcccag tcttcctgga tttcgccaaa atcccaggtc
    36301 gggataccca gaatgagcac atcgtactgc tccattaatt tcggggagtc gtccttgagg
    36361 ttatgtaagg tcaccagttc tgggccgata atatcgcgga ttttttctgc cgccatttcg
    36421 gtgtaacagg tgctggaacc gtaaaaaaga cccatattca taacgtaagc ttctcgattc
    36481 tgctgtggct tttgcggcta gtgtatcaga atcgctttag tcacggcaca ataaggcata
    36541 atggatcaaa tgagtgacca taaggggcgc aagtgaaaca ggatctggca cgcatcgagc
    36601 agtttcttga tgctctgtgg ctggaaaaaa atctggctga aaatacgttg aacgcttacc
    36661 gtcgcgatct gtcaatgatg gtggagtggt tgcatcaccg cgggttgacg ctggcgacgg
    36721 cgcaaagtga cgatttgcag gcattactgg cagaacggct ggagggcggg tataaagcca
    36781 ccagctcagc gcgtttgctg agtgcagtgc gccgattgtt ccagtatctt tatcgcgaaa
    36841 agtttcgtga agatgatccc agtgcgcatc tcgcttcacc gaaattgccc cagcgtttgc
    36901 caaaagattt aagcgaagcg caggtcgaac gtttattaca ggcaccatta attgatcagc
    36961 cactggagct acgcgataaa gccatgcttg aagtgttgta tgctaccgga ctgcgtgtct
    37021 ctgaactggt cgggttgaca atgagtgata tcagcctgcg tcagggcgta gtgcgggtca
    37081 ttggtaaagg caacaaagag cgcctggtgc cgttaggtga agaggcggtt tactggctgg
    37141 aaacctatct ggaacatggg cgtccgtggc tgttgaatgg tgtgtcaatt gacgtgttgt
    37201 ttcccagcca gcgtgcgcag cagatgacgc gacagacctt ctggcaccgc attaaacatt
    37261 atgccgtgct ggcgggtatc gacagcgaaa agctgtcacc gcatgtgttg cgtcacgctt
    37321 ttgccactca cttattaaat catggtgcgg atttgcgcgt ggtgcagatg ctactgggcc
    37381 acagcgatct ctccaccacg caaatttata cgcatgtcgc taccgagcgt ctgcggcaac
    37441 ttcatcaaca gcatcacccg cgggcgtgat gtctgaaaag aacaggaaaa tttatgaaga
    37501 aaggttttat gttatttacc ttgttggcag cgttttcagg ctttgttcag gctgatgacg
    37561 cggcaattca acaaacgtta gccaaaatgg gcatcaaaag cagcgatatt cagcccgcgc
    37621 ccgtagctgg catgaagaca gttctgacta acagcggtgt gttgtacatc accgatgatg
    37681 gtaaacatat cattcagggg ccaatgtatg acgttagtgg cacggctccg gtcaatgtca
    37741 ccaataagat gctgttaaag cagttgaatg cgcttgaaaa agagatgatc gtttataaag
    37801 cgccgcagga aaaacacgtc atcaccgtgt ttactgatat tacctgtggt tactgccaca
    37861 aactgcatga acaaatggca gactataacg cgctggggat caccgtgcgt tatcttgctt
    37921 tcccgcgcca ggggttggac agcgatgcag agaaagaaat gaaagctatc tggtgtgcga
    37981 aagataaaaa caaagcgttt gatgatgtga tggcaggtaa aagcgtcgca ccagccagct
    38041 gcgacgtgga tattgccgac cattacgcac ttggcgtcca gcttggcgtt agcggtactc
    38101 cggcagttgt gctgagcaat ggcacacttg ttccaggtta ccagccgccg aaagacatga
    38161 aagaattcct cgacgaacac caaaaaatga ccagcggtaa ataattcgcg tgaaacaaca
    38221 gatacaactt cgtcgccgtg aagtcgatga aacggccgac ttgcccgctg aattgcctcc
    38281 cttgctgcgc cgtttatatg ccagccgggg tgtgcgtagc gcgcaggaac tggaacgcag
    38341 tgttaaaggc atgctgccct ggcagcaact gagcggcgtc gaaaaggccg ttgagatcct
    38401 ttacaacgct tttcgcgaag gcacgcggat tattgtggtc ggtgatttcg acgccgacgg
    38461 tgccaccagc acggctctaa gcgtgctggc gatgcgctcg cttggttgca gcaatatcga
    38521 ctacctggtt ccaaaccgtt tcgaagacgg ttacggctta agcccggaag tagtcgatca
    38581 ggctcatgcc cgtggcgcgc agttaattgt cacggtggat aacggtattt cctcccatgc
    38641 gggggttgaa cacgctcgtt cgttgggcat tccggttatt gttaccgatc accatttgcc
    38701 aggcgacaca ttacccgcag cggaagcgat cattaaccct aacttgcgcg actgtaattt
    38761 cccgtcgaaa tcactggcag gcgtgggtgt ggcgttttat ctgatgctgg cgctgcgcac
    38821 ctttttgcgc gatcagggct ggtttgatga gcgcggcatc gcaattccta atctggcaga
    38881 actgctggat ctggtcgcgt tggggacagt ggcggacgtc gtgccgctgg acgctaataa
    38941 tcgcattctg acctggcagg ggatgagtcg catccgtgcc ggaaagtgcc gtccggggat
    39001 taaagcgctg ctggaagtgg ccaatcgcga tccacaaaaa ctcgccgcca gcgatttagg
    39061 ttttgcgctg gggccacgtc tcaatgctgc cgggcgactg gacgatatgt ccgttggcgt
    39121 ggcgctgttg ttgtgtgaca acattggcga agcgcgtgtg ctggcgaatg aactcgatgc
    39181 actaaaccaa acacgtaaag agatcgaaca gggaatgcag gttgaagcgc tcactctgtg
    39241 cgagaagctg gaacgtagcc gcgacacgct gccaggtggg ctggcaatgt atcaccccga
    39301 atggcatcag ggcgttgttg gcattctggc atcgcgtatc aaagagcgtt ttcaccgtcc
    39361 ggttatcgcc tttgcgccag ctggtgatgg tacgctgaaa ggttccggtc gctccattca
    39421 ggggctgcat atgcgtgatg tgctggagcg attagacaca ctctaccccg gcatgatgct
    39481 caagtttggc ggtcatgcga tggcggcggg tttgtcgctg gaagaggata aattcgaact
    39541 ctttcaacaa cggtttggcg aactggttac tgagtggctg gacccttcac tattgcaagg
    39601 cgaagtggtt tcagacggcc cgttaagccc ggccgaaatg accatggaag tggcgcagct
    39661 gctgcgcgat gctggcccgt gggggcagat gttcccggag ccgctgtttg acggtcattt
    39721 ccgtctgctg caacagcggc tggtgggcga gcgtcatttg aaagtcatgg tcgagccggt
    39781 cggcggcggt ccgctgctgg atggtattgc ttttaatgtc gataccgccc tctggccgga
    39841 taacggcgtg cgcgaagtgc aactggctta caagctcgat atcaacgagt ttcgcggcaa
    39901 ccgcagcctg caaattatca tcgacaatat ctggccaatt tagcgtcatc ttctctataa
    39961 aaaagagcgt ggattgggta caatcccgct cttatcaccg cattttgact agctcaataa
    40021 aagaaatcag accatgtttg aaattaatcc ggtaaataat cgcattcagg acctcacgga
    40081 acgctccgac gttcttaggg ggtatctttg actacgacgc caagaaagag cgtctggaag
    40141 aagtaaacgc cgagctggaa cagccggatg tctggaacga acccgaacgc gcacaggcgc
    40201 tgggtaaaga gcgttcctcc ctcgaagccg ttgtcgacac cctcgaccaa atgaaacagg
    40261 ggctggaaga tgtttctggt ctgctggaac tggctgtaga agctgacgac gaagaaacct
    40321 ttaacgaagc cgttgctgaa ctcgacgccc tggaagaaaa actggcgcag cttgagttcc
    40381 gccgtatgtt ctctggcgaa tatgacagcg ccgactgcta cctcgatatt caggcggggt
    40441 ctggcggtac ggaagcacag gactgggcga gcatgcttga gcgtatgtat ctgcgctggg
    40501 ctgaatcgcg tggtttcaaa actgaaatca tcgaagagtc ggaaggtgaa gtggcgggta
    40561 ttaaatccgt gacgatcaaa atctccggcg attacgctta tggctggctg cgtacagaaa
    40621 ccggcgttca ccgcctggtg cgtaagagcc cgtttgactc cggcggtcgt cgccacacgt
    40681 cgttcagctc cgcgtttgtt tacccggaag ttgatgacga tatcgatatc gaaatcaacc
    40741 cggcggatct gcgcattgac gtttatcgcg cgtccggcgc gggcggtcag cacgttaacc
    40801 gtaccgaatc tgcggtgcgt attactcaca tcccaaccgg gatcgtgact cagtgccaga
    40861 acgaccgttc ccagcacaag aacaaagacc aggccatgaa gcagatgaaa gcgaagcttt
    40921 atgaactgga gatgcagaag aaaaatgccg agaaacaggc gatggaagat aacaaatccg
    40981 acatcggctg gggcagccag attcgttctt atgtccttga tgactcccgc attaaagatc
    41041 tgcgtaccgg ggtagaaacc cgcaacacgc aggccgtgct ggacggcagc ctggatcaat
    41101 ttatcgaagc aagtttgaaa gcagggttat gaggaaccaa catgtctgaa caacacgcac
    41161 agggcgctga cgcggtagtc gatcttaaca atgaactgaa aacgcgtcgt gagaagctgg
    41221 cgaacctgcg tgagcagggg attgccttcc cgaacgattt ccgtcgcgat catacctctg
    41281 accaattgca cgcagaattc gacggtaaag agaacgaaga actggaagcg ctgaacatcg
    41341 aagtcgccgt tgctggccgc atgatgaccc gtcgtattat gggtaaagcg tctttcgtta
    41401 ccctgcagga cgttggcggt cgcattcagt tgtacgttgc ccgtgacgat ctgccggaag
    41461 gcgtttacaa cgagcagttc aaaaaatggg acctcggcga tatcctcggc gcgaaaggta
    41521 aactgttcaa aaccaaaacc ggtgaactgt ctatccactg taccgagctg cgtctgctga
    41581 ccaaagcact gcgtccgctg ccggataaat tccacggctt gcaggatcag gaagcgcgct
    41641 atcgtcagcg ttatctggat ctcatctcta acgatgaatc ccgcaacacc tttaaagtgc
    41701 gctcgcagat cctctctggt attcgccagt tcatggtgaa tcgcggcttt atggaagttg
    41761 aaacgccgat gatgcaggtg atccctggcg gtgccgctgc gcgtccgttt atcactcacc
    41821 ataacgcgct ggatctcgac atgtacctgc gtatcgcgcc ggaactgtac ctcaagcgtc
    41881 tggtggtcgg tggcttcgag cgtgtattcg aaatcaaccg taacttccgt aacgaaggta
    41941 tttccgtacg tcataaccca gagttcacca tgatggaact ctatatggct tacgcagatt
    42001 acaaagatct tatcgagctg accgaatcgc tgttccgtac tctggcacag gatattctcg
    42061 gtaagacgga agtgacctac ggcgacgtaa cgctggactt cggtaaaccg ttcgaaaaac
    42121 tgaccatgcg tgaagcgatc aagaaatatc gcccggaaac tgacatggcg gatctggaca
    42181 acttcgactc tgcgaaagcg attgctgaat ctatcggcat ccacgttgag aagagctggg
    42241 gtctgggccg tatcgttacc gagatcttcg aagaagtggc agaagcacat ctgatccagc
    42301 cgaccttcat tactgaatat ccggcagaag tttctcctct ggcgcgtcgt aacgacgtta
    42361 acccggaaat cacagatcgc tttgagttct tcattggtgg gcgtgaaatc ggtaacggct
    42421 ttagcgagct gaatgacgcg gaagatcagg cgcagcgctt cctggatcag gttgccgcga
    42481 aagatgcagg tgacgacgaa gcgatgttct acgacgaaga ttatgtcacc gcactggaac
    42541 atggcttacc gccgacagcc ggtctgggaa ttggtatcga ccgtatggta atgctgttca
    42601 ccaacagcca taccatccgc gacgttattc tgttcccggc gatgcgtccg gtaaaataag
    42661 cattacgtta tgctcacaac cccggcaacc gtcggggttt tttatttaag ctgggtaaat
    42721 gcagataatc gttttctggc ttcgcgattt gtcgcctgca tcaccatcca cggactgaac
    42781 gcccacggcg tagcatcaat accgcgtaaa accgctgcta aatcacacca ttgataatcc
    42841 atcacttcat catcgttgat ctgtaacgca ctggtcgtgc gtgcggcaaa taccggacac
    42901 acttcatttt ccacaatgcc attcggatcg gtggcgcggt agcgaaagtc aggatagata
    42961 gattcaggag gcgtaatttc tacgccaagc tcataacggc aacggcggat caccgcctct
    43021 tcgttgcttt ctcccagttg tgggtgccca caaaccgagt tagtccacac gccaggccat
    43081 gcttttttgc taagggcgcg gcgggtaact aataattgcc ctttggcatt aaacagccaa
    43141 ctggaaaacg cgagatgtaa gagggtgtct gccgtgtgtg cggcatactt ttccagcgta
    43201 cccgtgggaa ctccctgtgc attcaataaa atgacgtgtt ccgtttgcat aatttctcac
    43261 atgtaattct gatcactcat cattatagat aatagaagag tgaaatgatt aaccaagtga
    43321 gtgtttatcg acaaccgccc gttttgagcg gatgtcgaca ggtaaaaacc atttaaaaaa
    43381 gcagcatcat atatttgaag cgactttcaa tggccattgc aacacaatca gaaatgacag
    43441 gaatagagag agtaaaaagc aacagcagac tgaatatagc gcgcattaaa agattcatat
    43501 tattctccat gctcattttt cttcagcttg cgcatcagaa gccacatacg tactgtcctg
    43561 acttttaaat ctttatcaga gacggattcc ttcaccaggt cagcatgatg ctggtagcca
    43621 ttaaagaaga cgttcagcac aacggcgcta agggttgcga gcataatgcc gctatgcagc
    43681 aacggttgca gtacggccgg taacttagaa aagaaatcgt gagagagcgt tggcgtcatg
    43741 ccaacaccga gactgatcgc cacaatatag aggttgtaac ggttagtggt gtagttacag
    43801 cgcgacagaa ttcgaatccc tgtcgccagt accatgccga acatcaccag ccccgcgccg
    43861 cccagcacaa attgcggaat cgaggccacc agcaccgcca ttttgggcac catgccgaac
    43921 aggatcaaaa tgataccgga agcaatgcac acccagcggc tatgaacgcg cgtcacgctg
    43981 accagcccga cgttctgcga aaatgacgta tgtgggaagc tgttaaacgt gcctccaatc
    44041 attgtcccga ccccatctac gcgtaaccca cgaataatgt cctgggaaga gagtttgcga
    44101 ccgactattt cacccagtgc caggaacatc cccatcgact cgataaacac gatgattaac
    44161 acggcagtca tggtcagaat ggaaacggga tcgaaaatcg gcataccaaa tgacatcggc
    44221 gtgacaatcg caaaccatga agcatcatgt agcccggata aattgacttc attcatcatc
    44281 cacgaaagta aaaagccaaa tacaatcccc agtaatacgg cgacgttgga cataaatcct
    44341 ttcgcatagc gagtaatgag caagataaaa attaagacgg caaaggagat acctaaataa
    44401 acgggattac catattgcgg attcccttta cctccggcgg cccagtcaat acccacctga
    44461 atgatgctaa gcccgataga agtaataacc acaccggtaa ccagtggcgg gaataaaggc
    44521 atcaagcgac cgataagtgg cgctaataat gtggtgataa aacccgcggc gatagtggca
    44581 ccgaatatcc ccagcaggcc gatatccggg ttcataccaa tggctatcat tggtgttaca
    44641 gcggcgaagg tcaccgacat aatcaccggc aggcggatcc ccataaagcg gccgataccg
    44701 atacattgca ataatgtgac gatcccgcag caaaagagat ccgagctaat gagcatcgca
    44761 atagcttctt ttgataggcc gagtcggtca ccaatcataa gaggaacagc gactgcacct
    44821 gcgtacatga caaggacgtg ttgcagaccg agtatgatca gctttcctgg tgataatatg
    44881 cggtcaacct catcagttgg gcggtcttgc ccagaagatg agggaagttg ggaatctatg
    44941 gcgctcatca gtgtttctcc tttcgcccgc cagagtaaaa agcaaccgtt tatgttcatg
    45001 aaactgtcta aacacatgct cgtggagtgc cagtttttgc gaggaagggg agttagagac
    45061 aggaattgcg gaggcaatca cataatcgtg gttaaaccac gattatgaga agagaacctg
    45121 acaatggaat tatctgtaat tttctcatat tgatatttca ttaatcattc tggcatgtcg
    45181 cactctcgca tttaatcgtt tttatctgga tagcattctt ttgatcggca atgttgaaat
    45241 aatgaaagcg gcatagattg aggggcaata atttgaactc agttgcaaaa ttataattaa
    45301 aacggtcatg ctacctaata tatgcccgtt tattgcatcg cgaaatttga gaagacataa
    45361 ctcattaaat gaaagggatg caaatgaata agtttatcgc tgctgaagct gcggaatgta
    45421 taggctgcca tgcttgtgaa attgcctgtg cggtggcaca taatcaagaa aactggccgc
    45481 tgagtcacag tgactttcga ccgcgtatcc atgttgtagg gaaaggccag gctgcgaatc
    45541 ccgtggcctg ccatcactgc aacaatgccc cttgcgttac ggcttgtccg gttaatgctc
    45601 tgactttcca gtccgatagc gtacaactgg acgagcaaaa atgtattggt tgtaaaagat
    45661 gcgcaatcgc ttgccccttt ggcgtcgttg agatggtcga tacgattgca cagaaatgcg
    45721 acctttgtaa ccagcgcagt tccggcacgc aagcctgtat tgacgtctgc ccaacacagg
    45781 cgttacggct gatggacgat aaagggttgc agcagataaa ggtggcccgc cagcgcaaaa
    45841 cggcagcagg aaaagcgtta tcagacgctc agccatctcg cagtgcagcg ttgctccccg
    45901 ttaactcgcg taaaggcgca gataaaattt cagcgagtga acggaaaacc cactttggcg
    45961 aaatctattg cgggctggat ccacaacaag cgacttatga gagtgaccgc tgtgtttatt
    46021 gtgccgaaaa agctaactgc aactggcatt gcccgctgca taacgctatt ccggattaca
    46081 tccgtctggt acaggaagga aagattattg aagcggcaga actttgccac cagaccagtt
    46141 ccttacccga aatctgcggc agggtatgtc cacaggaccg tctttgtgaa ggcgcatgta
    46201 ctttgaaaga tcactctggc gcagtctcta tcggtaatct ggaacgctac atcaccgata
    46261 ctgcgctggc gatgggctgg cgtcccgatg tcagcaaagt tgttccccgt agcgaaaaag
    46321 tggcggtgat tggcgctgga cctgcaggat tagggtgtgc tgatattctg gcgcgcgcgg
    46381 gagttcaggt tgatgtcttt gatcgccatc cagaaattgg cggtatgctg acttttggca
    46441 ttcctccttt caaactcgat aaaacggtat taagccagcg gcgagagata ttcaccgcaa
    46501 tgggaatcga tttccatctc aactgtgaaa ttggccgcga tatctctttc aacgaattaa
    46561 cggcggaata tgatgctgtt ttcctcggcg tggggactta cgggatgatg cgagcagatc
    46621 tgccgcatga agatgcgcct ggtgtcattc aggcgttacc gttcctgacc gcccataccc
    46681 gccagctcat gggattgccg gagtctgcag agtatccgct gacggatgtg gaaggtaagc
    46741 gagttgtggt attgggcggt ggcgatacga caatggattg tttgcggact tccatccgcc
    46801 ttaatgctgc cagcgtgacc tgtgcgtatc gtcgtgatga agtcagtatg ccgggttcgc
    46861 gcaaagaggt ggtcaatgcc cgcgaggaag gtgttgagtt tcagttcaat gttcagccgc
    46921 aatatatcgc ttgtgatgaa gatggacgct taacggcggt gggcctgatt cgtaccgcta
    46981 tgggtgagcc ggggccggat ggccgccgtc gtcctcgtcc ggttgcgggt tcagagtttg
    47041 aattgcccgc cgatgttctc attatggcct ttggtttcca ggcgcatgcc atgccgtggt
    47101 tgcagggcag cggaattaaa ctcgataaat ggggcctgat tcaaaccggt gacgtcgggt
    47161 atttacctac ccagacgcat ctgaaaaaag tctttgctgg tggtgatgct gttcatggcg
    47221 cggatctggt tgtcactgca atggccgcag gaaggcaggc ggcgcgcgat atgttaactc
    47281 tgtttgatac gaaggcatcg tgatgaaatc gttaattatc gttaatccgg ctgactgtat
    47341 tggctgccgt acttgtgaag tagcctgtgt ggtcgctcac ccttcagaac aggagttgaa
    47401 tgccgatgtc tttttacccc ggctgaaggt acagcgactg gactgcatca gtgcgccggt
    47461 gatgtgccat cagtgtgaaa acgccccttg tgttggcgct tgccccgtgg gggcgctgac
    47521 gatgggcgag caggtggtgc aggccaattc tgcccgttgt attggctgtc agagttgcgt
    47581 cagtgcgtgc ccgtttggaa tgatcaccat tcagtcattg ccgggggata cccggcaaca
    47641 aatcgtgaaa tgcgatctct gtgaacagcg ggaagagggg ccagcctgcg ttgaatcttg
    47701 cccgacacag gcgctgcagt tgcttactga aagagaactc aggcgagtcc gccagcagcg
    47761 tattgctgcc agcggtgaga atcctctgtg aaagaaatct ccgcgctgaa aggcgcggag
    47821 aatctattag attgcccaac cacccgcgta aaacgcgacc agtgcggcag taatgatcac
    47881 cgtaccaata ttcagctttt gccattcgcg cgcaaagacg cgacccacca ccagtgttac
    47941 aaagcccagc ataataccgg taacgatatt acaagtcaga acgatgaaca cggcacacac
    48001 caggccagcc attgcgtcaa taaaatcgtt gaaatccagc ttcgagacgt tgcttaacat
    48061 cagcaaaccc acgtacatca gtgcgggagc tgtggcataa ccggggatca gaaacgacaa
    48121 cggtgataaa aacagaatca tcaggaacaa cgcaccaaca acggttgcgg ttaaaccggt
    48181 tttccccccg gcggcggttc ctgccgctga ttcgatataa accgccgcgg gcgctgcgcc
    48241 caccaggccg gagaatattg aacttactga gtcactggtc agggctttgc cgccgttgat
    48301 gatctggttg tctttatcca acaaattcgc ctgaccggcg acggcacgga tggtgccagt
    48361 ggcgtcgaac actgcggtca tcaccaatgc cagtacactc ggaagtacag ttggctggag
    48421 tgcgcccata atatcgaggc tgaaaatcag agatttacca tcttcgccag tcaggcttgg
    48481 cattgccacc agaccgtggt acttcaccgc tgggtcaaag attaagccga tgatcgaaat
    48541 tgcaataatc accaacaaga tcccgccggg tacgcggcac ttttccagac cgaagatgac
    48601 cgccagcccc agtaagctca tcatcaccgg gaaggaggta aacgcaccga gcgccactgg
    48661 caagccttca atcgggtttt tgataaccat tcccacaccg ttagcagcaa tcagcagcag
    48721 aaacaggccg ataccgatac ctgtaccgtg agcgatcccc atcggcaaat tacgtaagat
    48781 ccaggtacgc acaccggtta cggaaatagc ggtgaagatg acgcccatca gaaagactgc
    48841 gcccagtgcg acaggaacgc taatttgttg cccgagtacc agactgaatg cggtaaacgc
    48901 cgtcaaggaa atagcgcaac caatcgccat tggcaaattg gcccataacc ccatcagcaa
    48961 cgagccgaag cccgcgacca gacaggtggc aacaaacaca gctgcgggag gaaaacctgc
    49021 tttgcccagc attcccggaa cgacgataac ggaataaacc atggccagaa aggtcgttaa
    49081 gccagccagt acttcctgac gaacggtact gccacgagcg gtaattttaa aataattatc
    49141 cagcgcgccc tgtggctttg gtgcgtccgg tgtttgtagg atgtctccag acataatgat
    49201 gtcctcacga ggatgttaaa gttttattat tagttgcgtt cgtacaccag acgaccatca
    49261 acgtaggtgc ggtagatcga acggtcatcg cccaacgtca tcatcacgaa caatttgtcg
    49321 actaaagaaa cagagttgtc atagcgcagc tgctgtagcg gagtggcagt gggttccatc
    49381 accacgaaat cagcctcttt gccaggtaaa aagttgccaa tcaaatcgtc aaggcccaga
    49441 gatttcgctc cgccgagcgt ggccaggtaa aacgcttcat atgccgagag gcgatagcct
    49501 tgtaattgca ataccttgta ggcttcgttc agcgtttgca gcatgttaaa agttgttccg
    49561 gcaccgatat ccgttcccat gcccactttg actttcttct gccatgcttt tttcaagttg
    49621 aataagccgc tgccgaggta aaggttggag gtcggacaga aagcaatgct ggatttggtt
    49681 tcgctgagac gatcccactc tttttcttcg agatggacgc agtgagcaaa gacgcagttt
    49741 ttaccggtca ggccgtactg atggtaaaca tcaagataac catcatggtc aggataaagc
    49801 gatttcaccc aggcaatttc atctttgttt tcacagagat gggtatgtac ccatgtatcc
    49861 ggatattctt ctttcaggcg ttgcgccatc gccatctgtt caggagatga ggtaggggcg
    49921 aagcgtggcg taatcgcata caacagacga ccatttttgt gccagcgttc aatcagttct
    49981 ttgctttggt gatagctgct ttcggcagtg tcgagcagat aatccggtgc gtttcggtcc
    50041 atcatcacct taccggcaat catacgcata ttgatatgac tggcggcttc aaacagcgca
    50101 tcaacggatt gtggatgaac agtgccaaac accagcgccg tggtggttcc gttacgtaaa
    50161 agctgcttga tgaagaacgc cgacatctcg cgggcgtact ctaaatcctc ataacgacgt
    50221 tcagtaggga aggtgtgttt attcaaccac tccagcaatt gttcaccata ggcccccacc
    50281 atttcacttt gcggataatg gatatgtgta tcgataaagc ccggtactat cagtttgccg
    50341 cgatagtcgc gcacgcgaat ggtgtcagga atttgatgct ttccgtcttc ccattcgcca
    50401 aaccattcca cttttccctg tttaatgagt aataaaccat cctcaataaa ccgcagcgca
    50461 gaggcaattt cttccgggtt atcgaccgta cgggtgacat caataaaact gcctcgtacc
    50521 gcttttaacg tgtgttctcc tgacatcatt gactccttta ctgttaatcc atctcttccg
    50581 ctgaggtaat accaggcaga acgttttcct gtcggtagcc accagggagg ataatattta
    50641 ataaaatcgc agttaacccg ccagcacaaa tagggttttc aactaataca taaatagagg
    50701 ctggcaatat tttaaaaatt tcgggatcgt aggagacgcc aagtcctaaa cctaaagacg
    50761 tggcgacaat aagtgtttct cggcgcttta aaccgttggt gatgatgatg cgaatccctg
    50821 cgatggcaat catggaaaac atcaacgtca ttgcgcctcc cagaactgcc gagggaatgg
    50881 tcgtaaaaaa gccgccaatc atcggaaata agccgaggat aaccagcatt accgcgatgg
    50941 ttcgcccgac ataacgtgaa gcgacgccag tcatctgaat aaccccatta ttttgcgcaa
    51001 acgtggttaa tggtaatgaa ccgacagcgg aggcgataac agaaaccaga ccatctgcca
    51061 gcacgccgcc tttcagtcgg gactgatact cttccccctg aatggggcgg cgggaaacca
    51121 ttgctgtggc ggtgatatcg ccgacagctt ccagcacgct aagcagataa atcgtgccaa
    51181 ccaccaggaa ctgatggaag ctaaaactaa agccgtattt gaacggatgc gggatggtga
    51241 ttaacggcaa attgcgcata ctgctgaaat ccaccatgcc caggcataac gatgcgatat
    51301 agccgacaca tagcccaatg gcgatccctc ccatgcgtag caacggactg cgacagcagt
    51361 taaagccgat caccacaatc aaaaccaata agccaacgcc gagatgttcg taattgccga
    51421 acgtaccgct gcttttggct gcaaatcctc caccaaaatc aataatgccg actttaatca
    51481 ggcttaagcc gatcatcagc accacaatgc cgctgacggt aggcgtaata acccgacgta
    51541 aatagggcag gataaaagaa gagccgacaa ccagaaatgc gccaacgaag gagacgccga
    51601 ggagcgacga catgattaac tcttcgtgaa aaccgtcgct tttcatgccg ctgcccagcg
    51661 caatcatgac cgtaacaaat gaaaaattga ctgactgaat tgaaagtaaa ccagaaccga
    51721 cgatgccgta gcggtttact tgtaaccagg taccaatacc agaggcgatc atcgccatag
    51781 aaacaagata ggcagttgtt tcagcggaaa gctgtaaggc cgcacccacg attaacgcgg
    51841 gggttaccat cggaacgaaa attgccaaca gatgggtaat ggcacctacg agagcctgat
    51901 gaaagggagg gcgatcctcc agttcaaata taaggtcaga acctgcatgg tttatatcag
    51961 acatcccttc actcctttta aagtaaattt gatcatctga attagtaaaa taaattccca
    52021 cgaatttggg gcgcaggacg ccttgagaac ggcttatctc attttcgcag tcactattta
    52081 gtcatcgttc aaacagagaa acaggccgcc gaagcggcca tttattactt atattttttc
    52141 cagcgcggtg agtattttct ccggtgtgaa atgccattcg cgtaaccaga tgccgcaggc
    52201 atcgtgaatt gcggtagcaa tcgccggagc ggcgccattt acaccgattt ccgagatcga
    52261 tttcgccccg aatgggccga ctttatcgtc gctcggcacc agcacagcgc ggaaatcacg
    52321 cggaatgtca ccaattttcg gtgcgccgta actgcgtaaa tcacgcgtta acgggtgacc
    52381 ttcggcgtca taaatgatct cttcgctcat actgtggccg atagcacgca gggtggcacc
    52441 gtagatttgt cccagcgcta actctggatt gaccggtgta ccgcagtcca gcaaggcgta
    52501 gaatttatcc aggcggattt cacccgtacg cgtgttgacg gcaacttcag cgaagtttgc
    52561 gccatacggg aaggcgaaat caggcgtgat ataactgcca gttcccacca gtgaaccaaa
    52621 gccggtgccg gtttcgcctt tatgggctat ctctccgaaa ctaacttcgc ctttcttgcc
    52681 gcgaacgacg cccggcgttg ctagttgaac atctgccact ggctcaccca acatttgcgc
    52741 gccgtggaac aggattttct cccgcagatt ttccgctgcc aaacgcgcag cgttacccga
    52801 gaagcaagta ccggacgagg catatgcgcc tttatcaaac aacgcatgat cggtatcacc
    52861 ggagataaca tgcacgtcct gcggggggca gtgcagcact tctgctgcca gtttcgtcac
    52921 cacagtatcc aaaccagtac caatatccgc accgccagaa tgaacgataa aggtaccgtc
    52981 tgattccagt ttgatcatgc agttagcctg atcgatatcc gggatacccg atttctgcat
    53041 gataatggcg acgccacgac cgatgtgcca gtcaccgttt tgcggtttcg gtgaagacca
    53101 ttggatcatc tcgcgaccct gacgcaggat ctcttccagt gcgcagctgg cggcggaagg
    53161 aacggatgtc ggcgctttac cttcaccgat tgcaccgaga attttcagct cttgcccttc
    53221 gtgtacccgg ttacgttcga taatttccag ttggtcgatc tgtaactgtt cagccagttc
    53281 cgctaatgcc atggtgatag cgaagttacc tttcggcgcg ccataaccct gataagcacc
    53341 atttgggcaa atgttgctgt agtaggtggt gacctgaaaa tcgacgttat cgcatggata
    53401 taacggcagc gacagcgccg gtccgttaca cggtacggtg agtgagtggt taccgtaagg
    53461 acccgtgttg gcgcggaaat ccatcttcac tgccgtcagg cgaccatctt ttttcgcgcc
    53521 cagtttgacg gtgactttcg cgacgtgacg agaggtgtta gcaataaact cttcttcacg
    53581 ggtgtagcgg aaaagtaccg gacgcccggt cacgcaagtt gcccaggcgc acacttcttc
    53641 cagcaggatg tcctgtttag agccaaaacc gccgcctaca cgttctttaa tgacatgaac
    53701 tttatgttgt ttcatgccca cgaggcgcgc gacctggcgg cgtaagtgcc atggtacctg
    53761 ggtggaggcg tggatcacca gacgatcgcc gtccatacgg gtaaagcaga tatgtgtttc
    53821 agtcgggcac tgctgcgcct gcgttgagtt ataggttcgc tcaatgatca catcggcatc
    53881 ggcaaagcct ttgtccatat cgccaatatg accatgaata ctggcagcga tatttttgcg
    53941 tgggcgagaa ccgatcggga agttgatgat catatgctcg ccgcgctggg ccgcatggct
    54001 gttatcatct tccagagtat ctggtgcacc agcaacatac accaccggtt catcgtgcac
    54061 gacaggcgca tcttccgcca ttgcctcgtc aatcgacatt accggcttaa gcacttcata
    54121 ttcaacgtcg atgagcttca gtgcttcgag cgcaatgtct tcactttcgg ctactaccgc
    54181 agcaacgcga tcgccgacgt gacgcatttt cttgccgaac atacggcggt caagcggtga
    54241 tggttccggt gcgctctgac cacccggggt atagtaaata tccgggcaat tcaggtgagt
    54301 aataacgtga acgacgcccg gtaaggcttc agctttgctg acatccagat gagtaatcag
    54361 tgcgtgagcg tgtgggctac gtaacatttt aatgacgcag gcgtcagccg ttacgcgatc
    54421 ttcaacatag cagggtttcg cctgcaccat tttcgcggca tcagttttag gataatgctt
    54481 gccaatgact tcaaggtcgt cacggaaagt cggagcgata tcaatggtgg cctgcggatt
    54541 atttttacgt gcaaccgcca gttcaatgac ctgatagtat tgctgccagc cagcatcgcg
    54601 gctaaacaga cctgagagcg cgtcgtcgat ctcttcgcgg gtaggtgcgt cgatgcgatc
    54661 gagaagatcg gtgataatca gagccgcagc tggatcgtta taaccagact gcaccacgcc
    54721 aacatcaacc atggcctgtt gaaccagact taactcattc catttgccca gagattctgc
    54781 ggtacgaata tctgccttct ctaactgtgc ggcgataagc aaggacgcgt taacgatatt
    54841 accgttaaag ataattgcgt cagaaccggc aaacccaaaa ccatcatcac tgttgcgtac
    54901 agagtgcatt cccatgttaa acaacagctt ttgcacgttt tcgcctggat taacggttag
    54961 ctcctgaggc gcgccattta aagtaaagtg gatgatcata cggcttcctc cccagcctgt
    55021 tggcagtcgg catacaggtc ggctaccaga actcccgtga tatagcgttt ataggccacg
    55081 ctgccccgca aatcttcctg cgggaaaatg gcgttggcga cagcctgttc aagtgcattg
    55141 ccttccagat tttgagtttc aacatcatgc agacgcagtg ctttactggc gacgccatcc
    55201 agcgcaattc gcataccgtc gtggtctgtt attgcaacgg ctgccgtcac gacggttaaa
    55261 cctgcctgag aacggctaat tttgcgagtc gcacaggtgc gatacggatc tttaatgata
    55321 atttcggtta acaggcgatc gcatgggcag gccaggtagt cctcgattga cagcgtttcg
    55381 ccgttgccaa aaaccagttc agcatccagt gccagcagga cgggaagcag cactgactct
    55441 tcttgccggg cggcaatttc accaccaatg gtcgactgat tacggatatg gcgagagtag
    55501 acaaaaccga gagcttcccg cagtgctgca ggaataaatc gcgcatcacg cagtgactgc
    55561 aagcgagaca ttgcgccaat tcgtagtgca ccgttatccc agtcaatcca gtccagttcc
    55621 agatcctgta aggaaatggc aatcttttta tcggtacggg ttggtgtagc attgagtttg
    55681 ctgcccccgg cgaaccagac ggcttcatcc tggtagcggc gcttcagttc cagcgcctgt
    55741 tcgacagagt cgggcctgaa aaattgttca atcatcgtct ttccttattt aaccctcttc
    55801 cctgcgggaa gagggtatct gtcatttatg ccagcgcatc catccgacgc cacatactgg
    55861 cagcgacttt tctggcttgc gcataaatgg aatcgcaatc gaagttaaac tgacggtctt
    55921 catagaccat cacaccattg accatcacgc tgtgaacgct gcctgagccc ataccgaaag
    55981 cgatatgccc ggcgatattg tctgccagca gcggcgtcgg cgagttgtaa tcgcaaatgg
    56041 ttaaatcagc tttgtaaccg gcttccagaa gcccaaattt cgcgccaaag ttgcggctca
    56101 tcagttcgtt accgttagtc aaggctttgg caaaactgtc aggccacagc ggaccacccg
    56161 catcgcgatg tttaaagaag gcaaatttca tctcttcaaa catgtccgaa ccaatgccgt
    56221 ccgttcccaa cgccagattg cggatgtcgc taaggtgatg gttgtatccg acatggttgt
    56281 tcatgtttga acgggcgtta tgcaccagga acgcatcgcg ctgattgagt agggcgatgt
    56341 catctttcga caagtacagc ccatgagcga ccagcgtttt gctgtcgatg agatcgaatt
    56401 gcgccagtcg tgccagcagg tctttgccgt accagtggtg actgtaggaa acgtcataaa
    56461 ggtcttccgc agcgtgaatg tgcaaaccac gacctgtggc tttcacggct tcacgcagca
    56521 tctccagacc ggcatccggc acggtaaacg gcgcgtgagc gccgatatga gcttccacca
    56581 gatacggctc ggttgctgct ttcttcgcct catcaatctg acgggcgaag cggatgtttt
    56641 cttctacacc ttcctgcaac tctttgatgc cgctgttacg gtcagtagtt tcaaaacagg
    56701 tcatcgcgcg caggccaact tttaaaaatg cgtcgcgcaa tgtggagagc gacccgccga
    56761 tataggctgg agaggcatgg tgatcgataa ccgatgtaca tccgctctta atcgcttcca
    56821 gggaacaaat cagtccgctg taatagagcg actcttcatc aagggcgcga tcgagccgcc
    56881 accagagatt tttcagcgtt gagatgaaat ccgggcaggg ggcgatgttt gccataattc
    56941 cgcgggaaag ccccgagtaa aaatggttat gcgagcagac aattcccggc atcacaatcc
    57001 gaccatgcat ctctttgtag ctggcatcgg gatagcgttg cgtcagggca tcgtcgatag
    57061 cgacaatcac atcattttcg atggcgatat caacgccttc ctgcactttc gccgggtgta
    57121 actgcactgc ggtgacattc ttcagaatca acatgattac acctccacgc ggcccagcag
    57181 ataatgatga tgctggtgga catggctgat gatgcggcac atatcgttca gctccggtgg
    57241 tacgttgttg aactgacctt cgctgtcgat gtttaacacc cagctttggt tattcagacg
    57301 tacgcgtacc cggcaatctt ccacaaggaa gcctgggttg ctgctgttat caaagtcttg
    57361 cgacaggctg aagacggtga ttttgtcttt gtacggttta ccgttccacg ggcagaattg
    57421 ggcgcagttg ccgcattcgt tgcagtaggc gtcgagatgc agcgtctgga aacggttctg
    57481 gaagtctggg accgcaatgg atacgttggc gcggttcggg cagacatcca cacacttgct
    57541 gcaaacgtag ttacattcaa ggcagcgagc ggcttcctgc gcgacaaacg cgtcacgatc
    57601 gtcactgttc accagagtga cagagatatc gcctttacgt tgatagattt ccgccggatt
    57661 gacgttattc cagtatttat cgttctggtg ggaacggata ttttcccgac tcaggatggc
    57721 atcggtcgcc cgacgcgcgg ttccgacagc agcgacaatg gaggatggtc cgcgctgtac
    57781 gtcgccgatc ataaagacgt cagtcagacg cgtttcgcca ttatggtcga cgtctggcca
    57841 gccgtttttg tccagcggca cgcccattgc attcagggct tcagtatcct gctgttcacc
    57901 aatggcggtg atcaggctgt ctacatgcag tgttactgtt tcgttggttt caaccggacg
    57961 gcgacgacct ttctcatcag gttcgccaag cgacataacg cgcaaggtta aggtgccatc
    58021 agcatcgaaa cgttccggat tattcaggaa acggagctcc acgccgtcgt gcaacgcttc
    58081 ttcatactct tcgcgccatg ctggcatctc ttgcagtgaa cgacggtaaa cgaccgttgc
    58141 tttttctacg cctggaacgc gtaacgccgc acgagcgcag tccattgcgg tgttacccgc
    58201 cccgacaacg accacatgtt tgcccagctt gagtgtggtg cccttgttgt attcacgcag
    58261 gaaggggagt gatttccaga cattttggtt gtcgcccgcc agtttcacac cgctattttt
    58321 atcggtaccg gtggcaatca gaacatagtg gaagccctga ttttttaact gttcaacggt
    58381 cagatccggt gagcagccat actcaaattt tacgccgtga gcggcaacaa aatcgatatc
    58441 gtgctgaatt aactctgcag gaatacggaa ctgaggaatg atatttttca ccacgccgcc
    58501 cgcattggct tcgcgttcaa acagcgtaac cggatggccc gctctggcaa ggaagtaacc
    58561 tgctgccaga cccgccggac ctgcaccaat cacggcaacc ggatggcgtg aaccagaacc
    58621 ggctggtttg tgccagcgtt gcttatattc atcccaacct ttttccagcg cgactttttt
    58681 cagttcgcgg atattcagcg cactgtcgta atccaggcgg gtacagttgt attggcactg
    58741 gtgatcgcaa atatgaccgg taatggcggg cagggcgttg cgttgataga tgagttccag
    58801 cgcgtcggca tagcggtgtt cgccaagcag acggatgtat tccggaatat cttgcttgat
    58861 agcgcaggca gtaacacagg gggcaacgta gcaatcggtc agcggcaggt cttcagccac
    58921 ttcaatacgc tcttctggct tccagtgttt ctgggtgtat tccatggtta acgcgtctgc
    58981 tgccagtctg ttcagtcgtt cgacgtcaac gtggttaagc tcccaggcat ctgagccttc
    59041 cagctcgcgc atgcaggcac ttaagcgcag atagccgcca ggtttcagca ggtcggttgc
    59101 catagtaata gggcgaatac cagtatcaaa aatatcgcgg atagtcagct gactggcacc
    59161 accggaataa gaaatgggca gtttgccgtc aaaggcgcga gagagaactg ctgcaacgtt
    59221 gatggagagc gggaacagcg cacggcctga catatacatc tcttcaccgg gcaatgcgcc
    59281 tttattgttg atggtgccga gagtgttagt cagttttacg ccaaagccga gtgatttttc
    59341 ttttgccagt gccatcaggc gttccagcat ttccagtgct tgcgtcagct tgaggtcgtg
    59401 atcaaatgac tcttctttta agccgatgta accgaaaccg cagacatcga ggatctcacg
    59461 aacacgcgca taccccagta aggtcgggtt aagtttcaca aaggtgttga gccctttttc
    59521 ttccagcatg tagcggcaaa tggcttcgat ttcatgcgga ggacagccgt gcatggtgga
    59581 gagggtgacg ccctgcacca tactggtggg gatgcgagcg ggtaaggctt gcaagctttc
    59641 gcgtttttcc tgcaatccgt ggcgggctaa aaatgcctca tcctggagta gcttattaag
    59701 tgcatcgcgg tattgagcga atttcggatg gtcagatgcg tccatcatat tgtcgatgaa
    59761 ctgctgcatc ggcggttgct taataccttc gaggttgtaa ccgacgctca tattaaagat
    59821 gaacgattta ccggaatcag aaggctggaa catcgcttcg agaaggtgca gggcaaacca
    59881 ggctttgagg tattcatccc aggctttaag cagggtaaat tcggtagacc attcggtgtt
    59941 aaagcactcg tcttcggcat cgatacaggg cttttccagc tccaggcggt caagaatttg
    60001 gacggttttt agttcgatga agcgtccgcc agtcagccag gaggtgacaa tattttgcgc
    60061 gagttgcgtg tgcgggccag cggcagggcc gacgggagtg gcacaggttt caccgaagac
    60121 gctaacagtt ttacctttta cgggtgagta aaactgttgc tcgggaatac caaagattga
    60181 gcgttgttgt tggtattcat caaatatgcg cgtcaaaagt tcctcaaacg gaatgggacg
    60241 cataatatcc cccataaccc tctccttagc ttatacagat atcattgagt gaccggtgtt
    60301 gagaaatccg gtggtttagg gggatagagc aacaatcgca ccactcttgc caggtttgac
    60361 gggaaatata aaaaaatgtg aggcggctcg aaacttaatg gctttttgtg aatgaactca
    60421 caggtgccaa atgagaatta aaatcactcc tgaaaattat tattgccagc acatttatca
    60481 ctttttctct tttctcaaa
//
LOCUS       DAZFJL010000009        59458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFJL010000009 DAZFJL010000000
VERSION     DAZFJL010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.009"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..178)
                     /locus_tag="ACHJ8C_000572"
     CDS             complement(<1..178)
                     /locus_tag="ACHJ8C_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO5550482.1"
                     /translation="MALNCPTCKTPLERNGETAHCATCDKDFTVQALCPECHQALQVL
                     KACGAVDYFCQHGHG"
     gene            complement(241..1713)
                     /gene="der"
                     /locus_tag="ACHJ8C_000573"
     CDS             complement(241..1713)
                     /gene="der"
                     /locus_tag="ACHJ8C_000573"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO5550483.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYALGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            complement(1831..3009)
                     /gene="bamB"
                     /locus_tag="ACHJ8C_000574"
     CDS             complement(1831..3009)
                     /gene="bamB"
                     /locus_tag="ACHJ8C_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO5550484.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTVWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVNLAEKDG
                     WFSKDPALLSGGVTVSGGHVYIGTEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(3020..3640)
                     /gene="yfgM"
                     /locus_tag="ACHJ8C_000575"
     CDS             complement(3020..3640)
                     /gene="yfgM"
                     /locus_tag="ACHJ8C_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO5550485.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(3658..4932)
                     /gene="hisS"
                     /locus_tag="ACHJ8C_000576"
     CDS             complement(3658..4932)
                     /gene="hisS"
                     /locus_tag="ACHJ8C_000576"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO5550486.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQVL
                     LNDAPALGYYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(5043..6161)
                     /gene="ispG"
                     /locus_tag="ACHJ8C_000577"
                     /gene_synonym="gcpE"
     CDS             complement(5043..6161)
                     /gene="ispG"
                     /locus_tag="ACHJ8C_000577"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO5550487.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(6188..7195)
                     /gene="rodZ"
                     /locus_tag="ACHJ8C_000578"
     CDS             complement(6188..7195)
                     /gene="rodZ"
                     /locus_tag="ACHJ8C_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO5550488.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELNNNQSQ
                     SVPLDTSTTTDQAMATTPTSPVDTTATNTQTPAVTAPAPAVDPQQNAVVPPSQANVDT
                     AATPAPAATTTPDGAAPLPTDQAGVTTPAVDPNALVMNFTADCWLEVTDATGKKLFSG
                     MQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSPA
                     Q"
     gene            complement(7480..8634)
                     /gene="rlmN"
                     /locus_tag="ACHJ8C_000579"
     CDS             complement(7480..8634)
                     /gene="rlmN"
                     /locus_tag="ACHJ8C_000579"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO5550489.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(8784..9215)
                     /gene="ndk"
                     /locus_tag="ACHJ8C_000580"
     CDS             complement(8784..9215)
                     /gene="ndk"
                     /locus_tag="ACHJ8C_000580"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO5550490.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGSIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(9364..11676)
                     /gene="pbpC"
                     /locus_tag="ACHJ8C_000581"
     CDS             complement(9364..11676)
                     /gene="pbpC"
                     /locus_tag="ACHJ8C_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO5550491.1"
                     /translation="MPRLLTKRGCWIMLAAPPFIIILAAWAADKLWPLPLQEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWMHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSRERVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSVNLPEDPRPDSVSRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(11677..16638)
                     /gene="yfhM"
                     /locus_tag="ACHJ8C_000582"
     CDS             complement(11677..16638)
                     /gene="yfhM"
                     /locus_tag="ACHJ8C_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO5550492.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQEFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVIEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNSARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGVEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVRFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLNLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSNSPAPVELAPGVRTTLFIPVR
                     ALPGYGDGDIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAL
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMISIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKIMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDSALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TLQDLPGKWQAQTTFSAEPLTGEKAQTSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLPASNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAADDLLIVRP"
     gene            16845..17690
                     /gene="sseA"
                     /locus_tag="ACHJ8C_000583"
     CDS             16845..17690
                     /gene="sseA"
                     /locus_tag="ACHJ8C_000583"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO5550493.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPEK"
     gene            18352..18477
                     /gene="timP"
                     /locus_tag="ACHJ8C_000584"
     CDS             18352..18477
                     /gene="timP"
                     /locus_tag="ACHJ8C_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO5550494.1"
                     /translation="MKIRCFCIVLIVSGALLSEVNNNRSLSGNNLLAVNNLQSSK"
     gene            complement(18509..19285)
                     /gene="sseB"
                     /locus_tag="ACHJ8C_000585"
     CDS             complement(18509..19285)
                     /gene="sseB"
                     /locus_tag="ACHJ8C_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO5550495.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQAAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(19404..20687)
                     /gene="pepB"
                     /locus_tag="ACHJ8C_000586"
     CDS             complement(19404..20687)
                     /gene="pepB"
                     /locus_tag="ACHJ8C_000586"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO5550496.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGTDDLGLI
                     QRAARKIDGLGIKHVQLSGEDWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAARLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(20746..20946)
                     /gene="iscX"
                     /locus_tag="ACHJ8C_000587"
     CDS             complement(20746..20946)
                     /gene="iscX"
                     /locus_tag="ACHJ8C_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO5550497.1"
                     /translation="MGLKWTDSHEIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(20958..21293)
                     /gene="fdx"
                     /locus_tag="ACHJ8C_000588"
     CDS             complement(20958..21293)
                     /gene="fdx"
                     /locus_tag="ACHJ8C_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO5550498.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(21295..23145)
                     /gene="hscA"
                     /locus_tag="ACHJ8C_000589"
     CDS             complement(21295..23145)
                     /gene="hscA"
                     /locus_tag="ACHJ8C_000589"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO5550499.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSTAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(23162..23677)
                     /gene="hscB"
                     /locus_tag="ACHJ8C_000590"
     CDS             complement(23162..23677)
                     /gene="hscB"
                     /locus_tag="ACHJ8C_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO5550500.1"
                     /translation="MDYFTLFGLPACYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(23773..24096)
                     /gene="iscA"
                     /locus_tag="ACHJ8C_000591"
     CDS             complement(23773..24096)
                     /gene="iscA"
                     /locus_tag="ACHJ8C_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO5550501.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(24113..24499)
                     /gene="iscU"
                     /locus_tag="ACHJ8C_000592"
     CDS             complement(24113..24499)
                     /gene="iscU"
                     /locus_tag="ACHJ8C_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO5550502.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(24527..25741)
                     /gene="iscS"
                     /locus_tag="ACHJ8C_000593"
     CDS             complement(24527..25741)
                     /gene="iscS"
                     /locus_tag="ACHJ8C_000593"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO5550503.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(25853..26341)
                     /gene="iscR"
                     /locus_tag="ACHJ8C_000594"
     CDS             complement(25853..26341)
                     /gene="iscR"
                     /locus_tag="ACHJ8C_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO5550504.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(26611..27351)
                     /gene="trmJ"
                     /locus_tag="ACHJ8C_000595"
     CDS             complement(26611..27351)
                     /gene="trmJ"
                     /locus_tag="ACHJ8C_000595"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO5550505.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQAEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            27470..28273
                     /gene="suhB"
                     /locus_tag="ACHJ8C_000596"
     CDS             27470..28273
                     /gene="suhB"
                     /locus_tag="ACHJ8C_000596"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO5550506.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            28418..29272
                     /gene="yfhR"
                     /locus_tag="ACHJ8C_000597"
     CDS             28418..29272
                     /gene="yfhR"
                     /locus_tag="ACHJ8C_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5550507.1"
                     /translation="MALPVNKRVLKILFILFVVAFCIYLIPRIAINFFYYPDDKIYGP
                     EPWSAESVEFTAKDGTHLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            29463..30743
                     /gene="csiE"
                     /locus_tag="ACHJ8C_000598"
     CDS             29463..30743
                     /gene="csiE"
                     /locus_tag="ACHJ8C_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO5550508.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKVFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(30735..31874)
                     /gene="hcaT"
                     /locus_tag="ACHJ8C_000599"
     CDS             complement(30735..31874)
                     /gene="hcaT"
                     /locus_tag="ACHJ8C_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO5550509.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTAMPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            32243..32665
                     /gene="yphA"
                     /locus_tag="ACHJ8C_000600"
     CDS             32243..32665
                     /gene="yphA"
                     /locus_tag="ACHJ8C_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO5550510.1"
                     /translation="MNSLRYFDFGTARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFARPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGTISLDRR"
     gene            complement(32825..33697)
                     /gene="yphB"
                     /locus_tag="ACHJ8C_000601"
     CDS             complement(32825..33697)
                     /gene="yphB"
                     /locus_tag="ACHJ8C_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO5550511.1"
                     /translation="MTIYTLSQGALKLEVSDQGGVIEGFWRDAAPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDAWLGQWQCVSRSEDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNKGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGKARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTISEMSLRVALL"
     gene            complement(33708..34802)
                     /gene="yphC"
                     /locus_tag="ACHJ8C_000602"
     CDS             complement(33708..34802)
                     /gene="yphC"
                     /locus_tag="ACHJ8C_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO5550512.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVVVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGMPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(34835..35833)
                     /gene="yphD"
                     /locus_tag="ACHJ8C_000603"
     CDS             complement(34835..35833)
                     /gene="yphD"
                     /locus_tag="ACHJ8C_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5550513.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFDV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPIN
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(35858..37369)
                     /gene="yphE"
                     /locus_tag="ACHJ8C_000604"
     CDS             complement(35858..37369)
                     /gene="yphE"
                     /locus_tag="ACHJ8C_000604"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO5550514.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIMDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(37392..38375)
                     /gene="yphF"
                     /locus_tag="ACHJ8C_000605"
     CDS             complement(37392..38375)
                     /gene="yphF"
                     /locus_tag="ACHJ8C_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO5550515.1"
                     /translation="MPKKMTRTRNLLLMATLLGSALFARASDKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKARVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFTQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(38472..41753)
                     /gene="yphG"
                     /locus_tag="ACHJ8C_000606"
     CDS             complement(38472..41753)
                     /gene="yphG"
                     /locus_tag="ACHJ8C_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO5550516.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHDDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYHEHQSQALPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVVYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDTGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPAL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVVKAIDAFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYGKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGVTPEKRLARLENNLGIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAVCANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLAAAGLGEVAHYESARAELMAINPAWPKAALFTTVMPFI
                     FSYVH"
     gene            41871..43063
                     /gene="yphH"
                     /locus_tag="ACHJ8C_000607"
                     /pseudo
     CDS             41871..43063
                     /gene="yphH"
                     /locus_tag="ACHJ8C_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(43127..44380)
                     /gene="glyA"
                     /locus_tag="ACHJ8C_000608"
     CDS             complement(43127..44380)
                     /gene="glyA"
                     /locus_tag="ACHJ8C_000608"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO5550517.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            44708..45898
                     /gene="hmpA"
                     /locus_tag="ACHJ8C_000609"
     CDS             44708..45898
                     /gene="hmpA"
                     /locus_tag="ACHJ8C_000609"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO5550518.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPNEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(45943..46281)
                     /gene="glnB"
                     /locus_tag="ACHJ8C_000610"
     CDS             complement(45943..46281)
                     /gene="glnB"
                     /locus_tag="ACHJ8C_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO5550519.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEENDAAI"
     gene            complement(46342..47676)
                     /gene="glrR"
                     /locus_tag="ACHJ8C_000611"
     CDS             complement(46342..47676)
                     /gene="glrR"
                     /locus_tag="ACHJ8C_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO5550520.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(47666..48379)
                     /gene="qseG"
                     /locus_tag="ACHJ8C_000612"
     CDS             complement(47666..48379)
                     /gene="qseG"
                     /locus_tag="ACHJ8C_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO5550521.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLVLLGCVQSHNRPAIDTPAEERIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGNWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQYHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTDEVTPDEP"
     gene            complement(48544..49971)
                     /gene="qseE"
                     /locus_tag="ACHJ8C_000613"
     CDS             complement(48544..49971)
                     /gene="qseE"
                     /locus_tag="ACHJ8C_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO5550522.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(50547..54434)
                     /gene="purL"
                     /locus_tag="ACHJ8C_000614"
                     /gene_synonym="purI"
     CDS             complement(50547..54434)
                     /gene="purL"
                     /locus_tag="ACHJ8C_000614"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO5550523.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFSEELGAVIQVRAADRAAVEAVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEGFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            54692..56248
                     /gene="mltF"
                     /locus_tag="ACHJ8C_000615"
     CDS             54692..56248
                     /gene="mltF"
                     /locus_tag="ACHJ8C_000615"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO5550524.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTTKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(56245..56748)
                     /gene="tadA"
                     /locus_tag="ACHJ8C_000616"
     CDS             complement(56245..56748)
                     /gene="tadA"
                     /locus_tag="ACHJ8C_000616"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO5550525.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(56806..57441)
                     /gene="yfhb"
                     /locus_tag="ACHJ8C_000617"
     CDS             complement(56806..57441)
                     /gene="yfhb"
                     /locus_tag="ACHJ8C_000617"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO5550526.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            57650..58498
                     /gene="yfhH"
                     /locus_tag="ACHJ8C_000618"
     CDS             57650..58498
                     /gene="yfhH"
                     /locus_tag="ACHJ8C_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO5550527.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVAMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            58554..58814
                     /gene="yfhL"
                     /locus_tag="ACHJ8C_000619"
     CDS             58554..58814
                     /gene="yfhL"
                     /locus_tag="ACHJ8C_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO5550528.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGEHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(59008..59088)
                     /gene="shoB"
                     /locus_tag="ACHJ8C_000620"
     CDS             complement(59008..59088)
                     /gene="shoB"
                     /locus_tag="ACHJ8C_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO5550529.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
BASE COUNT        14117 a        16504 c        14782 g        14055 t
ORIGIN      
        1 aaccgtgccc atgttggcag aaataatcca cagcaccaca agcttttaac acttgtaacg
       61 cctgatgaca ctcagggcaa agtgcctgga cggtaaagtc tttgtcgcaa gttgcacagt
      121 gagcagtctc gccattgcgt tcgagagggg ttttgcaggt tggacagttg agtgccataa
      181 atactcctcg cgtgttattc ggcttccctc tccgtgtgga aagggaaacc agataagtga
      241 ttacttactt ttcttgatat gcttcatcag acgcttacgt ttacgcatct gggttggcgt
      301 cagggtgtta cgcttgttcg catacgggtt ttccccttct ttgaactgaa tacgaatcgg
      361 cgatcccatt acgtccagcg atttgcggaa gtagttcatc aggtaacgct tgtaggaatc
      421 aggcaggtct ttcacctgat tgccgtgaat caccacaatc ggcgggttat aacccccggc
      481 gtgggcgtat ttcagcttca cacgacgacc gcgtaccagc ggtggttggt gatcttcaac
      541 agccatcgtc atgatgcgcg tcagcataga ggtccccaca cgacgggtgg agctgtcata
      601 cgcttcacgt actgattcaa acaagttacc aacaccactg ccgtgcaagg cagagataaa
      661 gtgcacacga gcaaaatcga taaagcccag acggaagtcc agcgtctctt tcacctgctc
      721 tttcacttcc tggctcaggc cgtcccactt gttcactaca atcacaagtg agcgcccact
      781 attgagaata aagcccagca aggagagatc ctgatcggaa ataccttcac gcgcatcaat
      841 caccaacatc accacgttgg catcttcaat ggcctgcaac gttttgatta ccgagaattt
      901 ctctaccgca tcggtgattt tgccgcgttt acgtacgcca gcggtgtcaa tgagcacata
      961 ctcacgtccg tcgcgttcca ttggaatgta gatactgtca cgcgtcgtgc caggcatgtc
     1021 gtaaacaaca acgcgctctt caccaagaat acggttagtg agtgtagact tacctacgtt
     1081 cgggcgaccc acaatcgcca gtttgatcgg cagactttgc gggtcgaagt cgtcttcctc
     1141 ttcttcttcg ccgttctctt ccgcttcaaa ttgcgcccag tattcggcgt cttcgtcgac
     1201 ttcttcttgc ggtgcgagat cttccatcca cggcagcagc acgtgctcca gcagacttaa
     1261 tacgccacga ccgtgagacg cggcgatcgg gtagatttca cctaaaccaa gcgcgtagaa
     1321 atcaaccact gcctgatcgg gatccagacc gtcagttttg tttgccacca ggaaggtcgg
     1381 tttttcacgg gagcgcagat gtttagcaat cgcttcatcc gccggcatca ggcccgcgcg
     1441 cgcatccacc ataaacagta cgacgtccgc ttcttcaatc gccagcagcg attgttccgc
     1501 catacgtgtc tctacaccgt cttcggtgcc atcaatcccg ccagtatcaa tacaaataaa
     1561 ctcgcggcct tcaatttctg cacgaccgta cttacggtca cgagtcagac ccgggaaatc
     1621 cgcaaccagc gcatctcggg tgcgagttag acggttaaat aacgtggatt ttcctacgtt
     1681 agggcgcccg acaagcgcga ccacaggtac catgtttaaa gcctcatttt tataaatcat
     1741 cagacaacgc acgctatatt cgtgtcgttg ttaaaaacag gaaaacggcc cctgtccagg
     1801 agccgttttc aaagtgaacg acagagacga ttaacgtgta atagagtaca cggttccgtc
     1861 ttttgcctgg atcagcagtt tgccgtcagc agcaaccggt tcagtctgga aaccggaact
     1921 atcaactttt tgctgggcaa cgaaacgacc atcttcgacg ttaatccagt gcagataacc
     1981 ttcactgtca cctaccacca ggttgccatt atacagcacc ggagaagtca gcaggcgatg
     2041 cagcagatcg ctttgtgtcc acagcgtaac gccgccatca atggtcaacg ccatcacccg
     2101 gtcattttga tcgaccagat agatgcgatt gccgtcgacg atgaaatcat tcaccgaacc
     2161 cagttcgcgt ttccacataa tctgaccgct gcgcagatca agcgccgtca ggttaccatt
     2221 ataggccagc gcgaaaacaa cgccgttaac aacgacggga gtcgtgtcaa catcgctcag
     2281 acggtcaatt tcggtagaac cggtcgcctg ggaaatacgc tgctgccaaa tcatctggcc
     2341 ctgttccatc agcactgcgc tgacgcgacc attatcgcca cccacgacgg ccgcaccaaa
     2401 agccgttgcc ggcgcagact cgccacgcaa agagagcgaa ggcatatcga ggttaactgt
     2461 ccatttgaca gcgccgtcag cttcgttcag cgcttgtaac tgaccgttac tggtgtggat
     2521 taacaccaga ccgtcgctga ccaccgggcg cgaaagtgct tcacccgcga ctttagtttg
     2581 ccatgccaca gtaccatcgc tggtattcag cgcgtaaacc tgcgcctttt cggtgccaat
     2641 gtagacatgc ccaccagaca cggtcacgcc gccagaaagt aatgcaggat ctttagagaa
     2701 ccagccatct ttctcggcca ggttgacaga ccagatttct ttgccgtcat ccgcattcag
     2761 cgcttttact aaaccagcgc ggtccgctgc atagacaacg ttgtccgcca gtgccggatg
     2821 aagattggaa tagaagttgc caatgccgct accaacggaa gtgctccaca cagtagtcgg
     2881 cgtaaactgg ttttcaacgg ttggcaatgg ggacatcttt accacatctt cttcgctgtt
     2941 aaacagcgaa cagccgctta aaagggtaac ggaaagcagt cctggcagca gtaatttacg
     3001 caattgcatc gggtccctct cagatggaca aattattaat tttcatctgc atcatttcgc
     3061 tcagtgccgg ggtaacatcg cttttcacgc ctgcttccca tgcactacgc gcaccttgct
     3121 tatcaccttt gctcagcaat gcttcaccac gcaggtcggc aacaatggca gcccaccctt
     3181 cacctttgat ggtatcaagg gtttttaacg cggcatcagc ctgcttgagc tgtacctgaa
     3241 cgcgagcaag acgcagattt atcacggctt tgagattttc atcgctcgtg tctgccagcc
     3301 cctgttgtaa ctgggcggca gctttctcta gttcattttt gtcaacaaat tgctgcgcaa
     3361 gttccaaaga agccagcgca ccataagtat ttttattttc agcagcaaat ttttccgccg
     3421 ctgggatgct atccggtttg ccttcgctca ctgcggtaac cgcattttga taggcaagag
     3481 aagcggagcg tgcagaatca acctgatggc tgttccagta gcgccagcca atcagtgcgc
     3541 caacgcccaa aatcacccca acagccagtg ctttgccatt ttcagcaaaa aagcgtttaa
     3601 ccgcttcaac ctggtcgttt tcgttctcgt aaatttccac gctgtccttc tccttcctta
     3661 acccagtaac gtgcgcaaat gcgcggctac gctatcctgc gcaactgcag tttgctcacc
     3721 agagcgcaaa tccttcacta ctgctgtgcc gttagccact tcagactcac ccagcaccac
     3781 agcaacgcgg gcaccccatt tatcagcacg ggcaaactgt ttcttaaagt tgccgccgcc
     3841 gtggttggtc atcagtttca cgcccggtaa ttcatcacgc agacgctctg ctaatgccat
     3901 agccgcagat tgtgtgtcag cacctgaagc caccaggtat atatcgacaa caggatcggc
     3961 tttaaattcc ggattaacgg cctgaactaa caatacaaga cgttcgaggc ccatcgcaaa
     4021 accgactgcc ggtgttgcac gaccacccag ttgttccacc agaccgtcat aacgaccacc
     4081 tgcacacacg gtgccctggg aaccgaggct gttagtcacc cactcgaaaa cggtacggtt
     4141 atagtaatcc agaccgcgca ccagacgctg gtttacggtg taagcgatcc ccgcgctctc
     4201 aagcagtttg cacagaccgg caaagtgctc acgagattcc tcgtccagat aatagcctaa
     4261 tgccggagcg tcgttgagaa gcacctgcac ttccggattt tttgaatcga gtacgcgcag
     4321 cgggttagtg tacatgcggc gtttgcagtc ttcgtccagc ttttctttat gctgctcaag
     4381 gaatgccacc agcgcatcgc ggtaattcgc acgagcttcc agcgaaccga tagagttcag
     4441 ctcaagagtt acgtgctcgg aaatacccag cgcgcgccac cagcgggcag tgagcataat
     4501 cagttcggcg tcgatatccg gaccttgcag accgaaaact tcgcagccca actgatggaa
     4561 ctgacgataa cgccctttct gcggacgctc gtgacggaac ataggcccga tataccacag
     4621 acgctgttcc tgattgtaca gaagaccatg ctcgatgccg gcgcgtacac agcccgccgt
     4681 cccttcaggg cgcagagtca ggctgtcgcc attgcgatcc tcaaaggtgt acatctcttt
     4741 ttcaaccacg tcggtgactt caccaatcgc acgtttgaat agcggggtct gctctacaat
     4801 cggcaagcgg atttcactgt aaccgtagct gccgagcacg tttttcagtg tgccttcaat
     4861 gcgctgccag atggccgttt cgccaggcag gtaatcgttc atgccgcgaa tggcttgaat
     4921 gttttttgcc acgtttattc tctttctgaa tataaaaatg aaccctcaac gcttccctca
     4981 atgtttcggg agccatgcgg gttcaatcat acacgggaag cgaggcgctt cccatcacgt
     5041 tattattttt caacctgctg aacgtcaatt cgacgcgctt cgtccagctg actggctttc
     5101 gcacgaatgc gtgcttccag ctggtcgatc atatcgttgt tgtccagacg gtctttgcgc
     5161 acgccatctt catagaggcc gcttttcttg ttgccgccag tgacgccgag tgtagaaacc
     5221 agcgcctcac ctgggccatt caccacgcag ccgataatcg aaacgtccat cggagtgatg
     5281 atatcttcca ggcgttgctc cagcgcgtta accgtaccga taacatcaaa ttcctgacgc
     5341 gaacatgtcg ggcaggcgat gaagttgatc ccgcgcgaac ggatacgcag cgatttcaaa
     5401 atatcgaaac cgactttgat ctcttcgacc ggatcggccg ccagcgatac gcgcagcgtg
     5461 tcgccgatgc cttcagacag cagcagacct aaaccaatag cggattttac tgccccgcta
     5521 cgcgcaccac cggcttcggt gatccccaga tgcagcggct gatcgatttg ttttgccagc
     5581 aaacgataag actcaacagc gaggaagacg tcagacgctt tcacgctgac tttgaactga
     5641 tcgaagttca ggcgatcgag atgatcaaca tgacgcatgg cagattccag caacgcctgc
     5701 ggcgtcggtt cgccatactt ttcttgcaga tctttttcca acgatccggc attaacgcca
     5761 atccggatcg gaatgttttt atcgcgcgca cagtcaacca ccatgcgaat acgctcttca
     5821 ttaccgatat tgccagggtt aatacgcaga caatcgacgc cgtattccgc tactttcagc
     5881 gcaatgcgat agtcgaagtg gatgtcagcc accagcggca cgttaacctg ctgtttgatg
     5941 agtttgaacg cttctgccgc gtccatcgtc ggtacggaga cacggacgat atcagcgcca
     6001 acacgttcca gcgccttgat ttgattgacc gttgcttcga cgtctgtcgt gcgcgtattg
     6061 gtcatggact gtacggcgat gggagcacca tcgccaatcg gcacattccc aacgtaaata
     6121 cgtgttgatt ttctacgttg aattggagcc tggttatgca tgaaaaatct cccgcgttac
     6181 ccgtctgtta ctgcgccggt gattgttcgg cattgagggt cagacgcgca acctggttag
     6241 ttctgataaa acgactcaga tcgacaggtt tcccttgata ctggatctgt actgcggctg
     6301 gcgcaccaat tttcagtttg tacggtgcct ggccggttaa gttcaaatta ccgtctttac
     6361 gctgcatacc gctaaacagt tttttaccgg tagcatcggt gacttccagc cagcaatcgg
     6421 cagtaaagtt catcaccagc gcattcgggt caaccgccgg cgtggtcacg ccagcctgat
     6481 cggttggcaa cggcgcagcg ccatctggtg ttgttgttgc cgctggtgcc ggagtcgcag
     6541 cggtatcaac atttgcctgc gaaggtggaa caaccgcatt ctgttgcgga tcaacagctg
     6601 gtgctggcgc agttacggca ggtgtttgcg tgttggttgc ggtggtatcc acaggtgatg
     6661 tcggcgtggt cgccatagcc tgatctgtgg ttgtcgacgt atctaacggt acactctggc
     6721 tttgattatt attcagttcc gcagaagatt gatcggccat agtggtgatc tcttcctgct
     6781 gagctttgtg gtcttgccac caccaggcac cgctcaggcc aataaccaca aacaatacca
     6841 gccaggtgaa ggtcatcagc cagccgtcac gttttttgcg gcgtttaccg agggaaaaac
     6901 tctgcatcgg cgcaactttt gcagcccgta gtggagcctg cttttccagc cctggcagca
     6961 gttcttcttc tggaatatgt accagacgcg cataagagcg gatatatccg cgcaggaatg
     7021 ttgaagcaag atcggcgggt gccttatctt cttcaatgtc gcgtaccgtg gaaaccttca
     7081 ggcaaagtcg ctcggcaacg gcctgttgac taagtcctag ttgttcgcga gcattacgca
     7141 ggcgagcgcc ggtagtaagt gcttcatttt ggtcgtgcgt ggcttcagta ttcattcgct
     7201 gcaggtacgt ttaaatgaga attgagatgc cggtgaacca tcatgcccac ccacaccgcg
     7261 aaacatccgt taagttaacc gttatcatac agtataagac cgtctgtgtg ctcgtgacaa
     7321 agcccacatt cgctaaggct aacttactgt tgcatcgtta catactgcct taaagtcagc
     7381 aaaaacgcac cgttaatatt gaccagacaa ttgcaactta atacttcatt cataaaagat
     7441 tacgccacgg tacataaagt aaccgtggcg taatggctat cagaccgctt taatgtcgat
     7501 ggcttcaccc tgcattcgtt tacgcagggt acgtttcgta cggtcgataa catcgcccgc
     7561 caactgacca caggcagcat caatgtcatc accacgcgtt ttacgcacaa tagtggtgaa
     7621 accgtagctc atcagcactt ttgagaaacg gtcgatacgg ctgttcgaac tgcgtccata
     7681 cggcgcgccc gggaacgggt tccacgggat caggttgatc ttacacggcg tatctttcag
     7741 cagttccgcc agttggtgcg cgtgttcagt gccgtcgtta acgtggtcaa gcatcacgta
     7801 ttcaatagtg actcggccct gattggcgtt ggatttctcc agataacggc gcaccgccgc
     7861 aaggaacgtt tcgatattgt actttttgtt gatcggcaca atttcgtcac gaatttcgtc
     7921 gttcggcgcg tgcagggaga tagccagcgc aacgtcgatc atatcgccca gtttatccag
     7981 cgccggaacc acaccggaag tggaaagcgt gacgcgacgt tttgacaggc caaaaccgaa
     8041 atcatcaagc atgatttcca tcgccggaac gacgttgttc aggttgagca gcggctcgcc
     8101 catacccatc atcacgacgt tagtgattgg gcgctgaccg gtgacttttg ctgcgccgac
     8161 aattttcgcc gcacgccaca cctggccgat aatttccgat acccgcaggt tgcggttaaa
     8221 gccctgctgg gcggtggaac agaatttaca ctccagcgca caccccacct gcgaagagac
     8281 gcagagcgtg gcacggtcgt cttccgggat atacaccgtt tcgacgcgct gatcgccaac
     8341 ggcaattgcc catttaatgg tgccgtcaga tgaacgctgt tcttcaacca cttccggtgc
     8401 gcggatctcc gccacctctt tcagtttgcc gcgcaacact ttgttgatgt cggtcatctc
     8461 atcaaagttg tcgcagcaat agtgatacat ccacttcatc acctgatcgg cgcggaaggg
     8521 tttttcacct aaatctttaa aaaactcccg catctgctga cggttgagat ccagcaggtt
     8581 gatttttcca tctttagtgg tgacgttttc aggtgtgact aattgttcag acatatgcta
     8641 ttccggcctc gttattacac gttatggccc ctggagggtt gaaaaaagaa acgccccggt
     8701 aagcggctgc tcgtccgggg gcgctgcatt gtacaaattc tggcgcacgg atgccacgtt
     8761 tgcacgcggc atttacgaaa ttattaacgg gtgcgcgggc acacttcgcc ttcgccgaag
     8821 aaataagcga tttcgcgagc ggcagattcg acggaatcag aaccgtgggt accattttcg
     8881 gtcaggctgt cagcgtaatc agcacgcaga gtaccggcca gtgcgtttgc cggattggtc
     8941 gcgcccagca gatcgcggtg acgctgaaca gcattttcac cttccagcac ggaaacaacg
     9001 atcgggccag aagtcatgaa ttcaaccaga ccatcaaaga acggttttcc atcgtgttca
     9061 gcataaaagc cacgagcctg ttcaacggtc aggtgcagca ttttggtgcc gacaattttg
     9121 aaccctgcag cttcaaagcg cgcaaagata ctaccaatca cgttttttgc taccgcgttc
     9181 ggtttgatga tggaaaaagt acgttcaata gccattttta cctctgtaaa ttgttctgtt
     9241 gttgtctgta ccagcgtacg agatggcgcg gattataatg agcaacagag ccgttgacta
     9301 ttgatgaagg taacattttt ttaaaataaa cccagtttaa gcaacaatca gggacaaaac
     9361 agactattgc atgacaaatt tcactgtcgc tatttgcccc acgtcatcca tcaccagcaa
     9421 ttgataatcg cctttatccg tcaaatgcag ggtcacgttg cgcccacgtt cagttaacgg
     9481 ttcgccgttc agaaaccacc agcgttcacc tgcccctcca cttgattgca acggcaaagt
     9541 cgcttccgct gcgcccggta aacgtttaat aatcgcgcca tcgcgcacac ctgttagctg
     9601 cagcgggagc tgtgcatcgt gaccgtatgg cgggcaaatt gtcgaggccg gtggcaagcg
     9661 cacagcgcga cgttctgatg cgggcagcca gggttccagc ggtagcggcc agacatttat
     9721 catttcttgt cgcgcctgag ggcaatcagc ggcaacacgt ttgccgtttt catccagcca
     9781 gatggggaaa cgaatgccat taatgccttc ctgctccggc agtaatagag ttggcggctg
     9841 gctcccgtcc agcagccagg ttgccaggcg gcggcgacag ttaccgtcac cttccggcaa
     9901 agactgtccg cccggccagc agataacgcc acgacttaca gagtcagggc gtgggtcttc
     9961 cggcaaattc acactgcgcg acagtaagat attattaacc tgattcaaca gcggtacggc
    10021 actggcaaag ccaaactgac caacaacggg cgtgccatcc ggtctgccag tccagatccc
    10081 aatgacatag cgagcgttaa ccccaatcgc ccaggcatca cgatagccat agctggtgcc
    10141 cgttttccat gccagtgggg cgacgcgcgg caaggcacca tccggcaagg gttgcgcttc
    10201 atcagccata atccggcgaa tgatccacgc agcccccgac gacattaaag gccgttcaag
    10261 cagcggatcg tcaggctgta agcgcaattt acctgccttg ccgtggcgag caaacgcggt
    10321 atacgctgcc gccatatctt ccagttttgc gccagcgccg ccgagaatga gcgaaagatt
    10381 cggtgcagca ccgttgggca gatacaacgg caatccaaca ttgcgtaact ttgccgcaaa
    10441 ccgtttcggt ccataggctt ccagcacctg cacagcaggt aagttcagcg atcgcaccag
    10501 cgcctcactc atgctgatcg ggccatgaaa accgctgtca aagttgccag gtcggtaatc
    10561 accggtgcgt cgggggacgt cttgcaacag cgatgccggg tggatcaagc cttcatccag
    10621 cgccagacca taaacaaacg gtttgagtac cgatcctggc gatcggatcg cattgaccat
    10681 atcaacatga ccaaagcgtg aatcatcgtt gagatcaacc gatcccaccc agccgcgaac
    10741 acgcatatcg gtatgatcaa ccacgatcat cgccagtgag ctgcgcggtg gcaatcgtcc
    10801 tttccagttt tgcgccagtt cttccagacg tcgttgaaga ccggcatcca gcgtagtaac
    10861 gattttgtcg cttttgcttt taccgagcat catgcgcgaa aacagcggtg ccagttgcgg
    10921 catttgtcgg ggtgccagcc agatgggttc ttcccttgac tcttttaccc gctcacggga
    10981 ccacacacct tgcacggcca tccgttcgag cactttatta cgcgcggctt cggcacgctc
    11041 cggccaacga tccgggcgaa gacggctggg cgcttgcggc aacaccgcca gcattgccgc
    11101 ctcggaatag cttaaattcg caggcgattt cccgaggtaa gcccaacttg ccgcaccaat
    11161 cccctgcaac gtaccgccaa acggggcacg gttaagatac aaggtcagaa tttcacgctt
    11221 agacagatgc cattccagtt gcaacgcgcg ccagagctgg cgaattttgc cgccaaacgt
    11281 tttgggatga ggatcaagca ggcgcgccac ctgcatggtg agcgtgctgc cgccggaaat
    11341 gacccgaccc gacgtgagat cttgccaggc tgcacgcgcc accgagaacg gattcacccc
    11401 cggatgcatc cagaaccagc gatcttcata attgatcagc gcttcaaggt aacgtggaga
    11461 aacatcttcg attgttaccg gataacgcca gatgccgtca gcatcggcga aacgccagag
    11521 cggcgtacca tcctgcgcca ccaccacgcg tgcaggattc acttcttgca atggcagagg
    11581 ccagagttta tctgctgccc atgctgccag aatgatgata aaaggcgggg ctgccagcat
    11641 tatccagcag ccgcgtttgg ttaacaggcg aggcatttac ggtctgacta tcaacagatc
    11701 gtcagccgca ccagtcgccc gccattgagg aacatacatt gattccacca taggctgcgg
    11761 cacctgatac gttcccggcg tcaccgcccg cgccagatac accagcgtta ccggttggta
    11821 ttcatcaacg gcaaccgccg ccacaaagcg atcgtcacgg aactctatgt gcttaatgct
    11881 cgcctgctgc atctggttca gcaagttctg cacttcacca ccactttgct ccaggctggc
    11941 gctgccgttc gccagattct ggttttccag ttccagcccc gcaggcaaca gatccacgac
    12001 taacgcatcc ggtacactgt tactggcttt aacctgcaac cacaccagca ccagatcgcc
    12061 gctacgtaac gagtccagcg atttgctctt accgtcagta cccagaatat gacgctcaat
    12121 ttgcagcaca ttgctcgccg gtaaaggtgc ggattgcgga taaccgctgg catccacacg
    12181 cagccataac ggctgatcgc cactgttggt cacctgcaag gtggcaagtt gatcgctatt
    12241 cagattgctg gtttgcgctt tctcgcctgt cagcggctca gcagagaaag tggtttgcgc
    12301 ctgccatttg cccggtaaat cctgaagagt acgggcagcc aggaacaacg cgttactttc
    12361 ctgcgtcgat agccagcgtt caccgaacgc ctgctgcgaa agtgtgttca acagggagta
    12421 ctgctcatcg ggtagcagtt tgttctcttc cagcaatgag agcatcaacg cgctgtcgcg
    12481 cagtggacta ccgtaatcac ccagccatat ccgctcatca ctattacgcg gcgttttcag
    12541 cgccagcaca atcgcttctt caccacgcgt tgcatcaccc atgattttca gcgcaacgcc
    12601 aagttgcagc agcggtaaac cagaagcggc atctgcacga tgctcccaga tttcacgcag
    12661 cgcacccagt ggtgcttttt gctgacgggc caacaccagc gcagcgtaag actgtacggc
    12721 gaatttactg gctttgagat tatcagcgta cgggatcgat atcatgcccg gatcttgtaa
    12781 atagcgcagc agacgctcat taccccggtt aatggcgtct gtcggcacgc tgtaaccctg
    12841 ttcgcctgcg cggaccagga aatccatcac gtaagccgtc agccagtact cttcgtcacc
    12901 gtttttatcc cacagcgcaa agccgccgtt atcacgttgc atttgcagca aacgggaaat
    12961 gccgatatcg accgatgcac ggcgtttctc atcactgtcg cctttgatgc ccaacgcctg
    13021 cagttgggct gcattggtat aaagtgacgg gaacaggccg ctggcggttt gctcgagaca
    13081 gccgtacgga tacgctttta actctttgat ataacgtgcg atgttcagcg gtggtttgcc
    13141 gctcaacaac aattgccctt ccagcgtaac aggcgagaag ttttgcaatc catcagccgg
    13201 gagcgcccag gtttcaccag gctgtaacgc cgtaccgtaa ttgaccgttt gtgccgggaa
    13261 tgccggacgg acgccgattt tccactgctt atgctgatcg gcaacggttt cccccggtaa
    13321 cgctaacccg ctaatggtgg cctgaatgtc tccatcgcca taacccggca atgcacgcac
    13381 cgggataaac agcgtagtac gcacccctgg cgctaattca acaggtgcgg gggaattact
    13441 caccagttca agcaagccac tggcggtcag agcaacatta agtttttgcg gtttatcggt
    13501 aagattagtg atatccagcg tcaggcgcga ggtatcgcca ctcgccataa agcgcggcat
    13561 gttcagttca gcaatcaccg gtgcggcaac aatcactttg ctttcgttgc ttccgaagtc
    13621 atctgccgtc caggcttgtg ccatgacgcg caattcaccg ttaaaatcgc caattggcag
    13681 tgtaaccgag ccttcgccct gttcgttgag cgttaccggc agcgcctgct gcgcgacaat
    13741 attgacgtga ttgaccggcg gtttaccacc acgtttcagc tcatcaccat caccaccaaa
    13801 acgcagagct gccagacgcc cctgaccttc aatgacctga ccgtaaatat cgtaaatgtc
    13861 tgcgccatag cgtttctgac caaagaacgc ctgccacgga tctggcgtga cataatcagt
    13921 aatattcagc acaccgctat caacggcaga caccagcaca ttcacctgtt ttggcatctc
    13981 accattttta ttgctggctt taattttcac ggttaatggc tgattggggc gcattttggt
    14041 gggggtttcc agcgccagat tgaggcgacg gttttcatca ccaagcggca gatgcaacac
    14101 cccaaccgcg cgttttggcg tcgcggagcg agatttatcg ccaggacgta ccaccagcgt
    14161 acttaaatac agatcatgac gattccaggt tttatcgacc ggaatcgtca gatccagccc
    14221 ttgagccgga acatcaatct cttgccacca cagcggccct tcactggact cgaccatcgc
    14281 ataaccttta cccgccgttg gcgcggcaat atgcaactta atggtatcgc cagggcgata
    14341 gctggcttta tccagtttca gcgtgacacg gtcgggtcgc acggcaccgc tcccgtcgct
    14401 gttgtcctgc cagctatagc cagcccagaa acgcacacta ctgaccgctt cattcggcgc
    14461 tttgacttcc agacgataag cgccccactc taccgggaaa ctgaccttgc cggtttcgtc
    14521 cgctttcaga tccagagttt gttcattttc gatcagatct ttttgatcaa actgtgactg
    14581 ccagccttca tcttctgacc agttccagta gtaatcgcgg cgttcgcgaa tcaggcgcac
    14641 ctgcaagccc gacacggctt tttttacgcc ttgcgcatcg ctataaacga tgtcaaaagc
    14701 ggcgttactg ccttcatcaa caatgggctg tttcaccgtg ctgtcagtgc gataatcgta
    14761 aaccgatttc gaggcgaact gcggacggat ccccggcaat gcatcggcag gccagatagc
    14821 ctgctcagcg cggcgcgtca ccgggcgacc gcccgattcc aacaaactac cctggaaaat
    14881 aacctgtaat ggggaatgcg tttccttcca ctggctttct gtagaaactt cgccacgccc
    14941 tttatcatcc agcgtcaact gaacttcatc cagcgtgcgg gaaagatttt cggcagctat
    15001 atcgccgaat tcgaaaccag gtaaggctga cacagcttca cgcagtgggc gcaggaaaag
    15061 ttgcccttgc aaagtattac cattagcagg cgcaccatac aggtagtacc ccaccacgga
    15121 gaatctcact tcatctttcg gcgttagcgg ggttttctca ccggtcagat tcagcgccat
    15181 gcgctctggc ataaaatctt cgacgtggaa atcccacatc cgatactggt tatcgcccgt
    15241 gttagcgcga atatgccaca taccggttgc cgcattgcta tcgagtggcc aggtaaagtg
    15301 gtagaggcca ttctccggct gactaacgac gctcctgagt acctgcccgt cgggtttaat
    15361 cacgtctaac ttgatgggtt gatcgggcaa tgctttacca tctgcatcac gcaataaacc
    15421 attgaggatg actgtttcac cagggcgata aagatcgcgc gggccaaaca tgaaaaactg
    15481 tttgctatag cctggcgcgc cagcaatgtt aaattctgct aagtccagcg ccggaagttt
    15541 taaatcgagt agcgttgtct gaccgtcttt acgcgccaac agtaatgccg cgtttttatc
    15601 attttccagc tgcacatgcc cctgggcatc actggttgcc tgagtcagag tctgcccttt
    15661 ctcatttaat aatgagactt caactccttg ctgggccgcg ccgttttcca ggctttgggt
    15721 aaagatatcc agacgattgt gataacggtg agctgaaacg ccgatatcac ttaacgtaaa
    15781 cagcgtcgcg ggattactgt aatcgtaacg tccagcctga ttcatcacag ccagatacac
    15841 gcctgcctgc tgaagcggtt tgatatcgcc cagcggcagc aataattttt cacgggtgtt
    15901 acgcgcagaa ttgagatcaa accgtccggt ataaaccaga tccgccatct gcagcagttt
    15961 gtcagactgc cagttcgcca gcgaattgcg gtattcccat tggctaatga atgcaggcag
    16021 agattctggc ttaacacgga agaagttaac atcgacattg ttaacgttga gcgccattac
    16081 cggcagccct tcaataactt taccaggtaa caacgagcca cggctggcaa aaccgacgct
    16141 gggttggata tctcgggtag ttatagtttt ttcgtaatct ttactgaagg ttgcgttgtt
    16201 gagtgctttg acctccttgc caatagtgac gatcaaatca cgtttcggtt cgaggtggcg
    16261 taaacgcaac tctttaagat tatctgacag ctcccaggca ccatccactt tgccgctttt
    16321 cttatcgacg acatggataa cgcgtgagaa ttcctgatcc gggtcgagag ggatggagaa
    16381 tgtcagcacc agcgtggctg cgccatcaag ttgaacttca gagagatcca gcaatgtcag
    16441 cgccttaccg gcactctgtt gggccagttt ttgtcgctct ggcgcggaaa gttttgctga
    16501 actcacgttt tctgaagagg ccgctttagt aacttcagaa ggcgcatctt ttttcaccgc
    16561 tgttggcgcg ttatcgttgt tgtcgcaccc tgccagcgcc agcattagca tgcaggcggc
    16621 tacgcgtaac tttttcattt ttcatccctg ccacaatggc ccgttagcaa cgtcgaataa
    16681 ttattatgcg tgagaattta cgttatgtaa ttcagtatca cgcccataca atccgaattt
    16741 tagtgagaat tgtggctcag acagccaaat cagcctcttt tgcttgtcgc ctttcactaa
    16801 acatccgaca atttagccta accccggcaa aaatggagat gcctatgtcc acgacatggt
    16861 ttgtaggagc cgactggctc gccgaacata ttgatgaccc ggaaattcag attatcgatg
    16921 cccgcatggc gtcgcctgga caggaagatc gtaacgttgc tcaggagtat ctgaatggac
    16981 atattcccgg cgcagtgttt tttgatatag aagcgctttc tgatcacact tccccgcttc
    17041 cgcacatgct gccgcgcccg gaaacgttcg ccgtggcgat gcgtgaatta ggcgttaacc
    17101 aggataagca cctgattgtc tatgacgaag gtaatctttt ctcagcccca cgagcatggt
    17161 ggatgctgcg cacctttggt gtagagaaag tgtcgattct gggtggtgga cttgcaggct
    17221 ggcagcgtga tgatctgcta ttagaagaag gcgcagtaga gctgccggaa ggagagttta
    17281 acgccgcgtt taatcctgaa gccgtggtga aagtaaccga tgtgttattg gcaagccatg
    17341 aaaatacggc gcaaattatc gatgcccgcc cggctgcacg ttttaacgca gaagttgatg
    17401 aacctcgccc aggtttacgt cgcggacata ttcccggtgc actaaatgtt ccgtggacgg
    17461 aactggtgcg cgaaggtgaa ctaaaaacga ccgatgaact ggatgcgata ttttttggtc
    17521 gcggcgtaag ttatgacaaa cctattatcg tcagctgcgg ctctggtgta acggcagccg
    17581 tggttttgtt ggcactcgcg acacttgatg tgccaaacgt gaaactgtac gacggcgcat
    17641 ggagtgaatg gggcgcacga gcagatttac cagttgagcc agagaaataa gcttttaaca
    17701 ggcaataaaa aaccgccgaa tctggcggtt ttttattgct ggtctggttc gcggcctttc
    17761 cagcagattg gcttgtgttg catgagcaac gcaggtgctt cacagcaaag caatactcgc
    17821 cagtaactct ctttttgtca agcgaaagag agtaatcatt gtttatttag cgtattatcg
    17881 acaccggccc tttccgccgt gttcggtaat aaaataacct ggcttattag tccgaattca
    17941 gacaaatata aacaaatcct gctcaaaatt aaaaatacta accggtaaaa gatattactt
    18001 aaacatgtaa attcactttc ctttaaaaaa caaaaaaccg ccaaaatcag gcggttattt
    18061 gttgctggtt tggttcgcgg cctttccagc agattgtatt accgtagtaa tgcaagcgcg
    18121 tctcagcgga cacaatactc gccagtaact ctctttttgt caagtaaaag agagttatta
    18181 ttgttctgtt agtgtattat ccactgcgga cctttccgcc gtctcgcaag cgggcgctgg
    18241 cttaaggaaa ggatgttccg cgaccgttag agcaagcgcg tctcagcgct tgctatcgcg
    18301 gcaatatcgc cagtggtgct gtcgtgatgc ggtcttcgca tggaccgcac aatgaagata
    18361 cggtgctttt gtatcgtact tattgtttct ggtgcgctgt taagcgaggt aaataataac
    18421 cggagtctct ccggcaacaa tttactggcg gttaacaacc ttcagagcag caagtaagcc
    18481 cgaatgccgc cctttggggc ggcatatttt agattatccg gttctgttta aagtcacgca
    18541 aaaaaccacc ccagcgacgt tcatagaatg gcgcaatatg ttcggtaata aagtggctaa
    18601 ttcctttttc cccttttttc acctgacaaa tatcgatggg ttcatcgcca ggtaatgtat
    18661 cggtcgctac acttcccgcc gcctgaataa tttcttcgat atcaccatcg gcttcaatgc
    18721 caataagtaa attaggctgt gcctcttcgt tctctttaat tgaacaaata aaagcacgct
    18781 tcaccggctt aatagtttta aataaggtgg tgagtgaatc aatcatttgt gctggtggct
    18841 ctgcgacttc cgataatatc agcgattcac cgccttccag tacttcctgg ctgctcagcg
    18901 gatttccttc ttcaccaatc agcaaactga tttcacgcgg cataaattct ttaccggttg
    18961 gcagtttggc attaaggaag agcgtttcgc caagggtcat ctcaaacaat gtgcgaacgg
    19021 gcattacgac aaatgcctgt tcgtcttcaa ccgcctgttg aagtgcttct aacgaggtaa
    19081 aaaaaggaat gacgctggtg ccgtcttctt tttcccagtg ctgtaaatca agcgcgctat
    19141 cttcaaccac agcctcgccc tgcgccgccg tacccggcac ccagacggtg gattccagta
    19201 gagtacggaa aaaggccggg cggtgcgccg gttcggttgc tgctttttcc agcaggtctt
    19261 caagttcgtt ttttgtttcg gacataagaa ccacaattca ttcaacgttc ggggcacaag
    19321 tgcctgatgc gctacgctta tcaggcctac aagggaatct caatctattg gattttcgca
    19381 attttgtagg ccaaataaga cgtttacgcc gttaacagat tagcaatcgt gcgcacacct
    19441 agcccggtcg cgcccgcaga ccactgttca accggcgctt tacggtaagt cgccgagcag
    19501 tcgatatgca gccagccttg ctgatagttc tcaacaaagt gcgacaggaa gcccgccgcc
    19561 gtgcttgcac ctgccggata cgccgcgctt ccggtattgt tcagttcggc aaagttagac
    19621 ggcagctggc tgcggtggaa ctccgccagc ggcagacgcc agaatggttc attctcctgc
    19681 gcggcactcg ccagcaagcg agcggcaagc gcatcgtcaa aactgaacag cgcgtgataa
    19741 tcattaccca gcgcggtttt cgccgccccg gtgagggtcg ccgcatcaat gatcagttcc
    19801 ggtttctgcg cactggcatc aatcaggcca tcggccagca ccagacgccc ttccgcatcg
    19861 gtgttcatca cttcaacttt tttaccgttg cgataggtaa tgatatcgcc cagcttgaac
    19921 gcattgccgc tgatcaggtt atccgcacag cagaggaaca gcttcacgcg cttgttcagt
    19981 ccgcgcgtaa tggcaaacgc cagcgctccg gtaaccgttg ccgcgccgcc catgtccgac
    20041 ttcatcgagt ccataaatgc ggtctgtttg atactgtagc cgccggaatc aaaggtgatg
    20101 cctttaccaa ccaggcacgc gtacactggc gcttctttat cgcctgttgg gttgtaatcc
    20161 agcgccagca ataccgggga acgttctgaa ccgcgcccga ccgtgtgcag ccccatataa
    20221 ccttgatcac gcagatcttc gcctttggtg atccggtaag tcacacgatc gcccgcgacg
    20281 ttgctgatca gatcaacagc acgctgtgcc agttgcgatg gccccagttc ttccgccggt
    20341 gcgttgatgg tgtcacgcac ccagtcgatg atcatcaggc ggttatccag ttcctgccgt
    20401 tgggcatcgt ccagatccgg ccactccact ttacgtgtgc ctttcggggc tttgtaacct
    20461 tgccagaatg cccagcagcg atccgcatcc cagtcttcac cgcttaactg aacatgcttg
    20521 atgcccagac cgtcaatctt gcgcgccgca cgctggatca gccccagatc gtctgtcccg
    20581 ttcaaatgca gggtaatgcc atcattatta atgctgtaag tcgctttttc tccccagcgc
    20641 gcgtcggcag gttgggtaga aagggtaatt ttcatcgctt ctgtcatttt agttatcctt
    20701 attcgtttaa cagcaaaacg ggctaccccc aggcagcccg ttcattcact cggcttcgtc
    20761 cagccagacc aacaaaatcg cttcgaggat tttctcgttc gatgcctgcg ggtcgtcgtc
    20821 gaaatcttcc agatcgcaaa tccactgatg catatcggtg aatcgaaccg ttttcggatc
    20881 aagatcggga tacgcatcgt acagtgcttc gccaatttcg tggctatcgg tccacttaag
    20941 tcccatacta acctctgtta atgctcacgc gcatggttga tagtgtaacg cgggatttcg
    21001 accactaaat cttcgtcggt aacgcgcgcc tggcagctta aacggctttc cggctccagt
    21061 ccccaggctt tgtccagcat gtcgtcttcc tgctctgagc tttccggcag cgagtcaaaa
    21121 ccttcgcgaa cgatgcagtg gcaggtggtg caagcacagg atttttcaca ggcgtgttca
    21181 atctcgatac cgttacgcag cgcaacgtcg agaatggttt caccactatt cgcttccaga
    21241 acagcgccat cagggcagag atcctgatga ggcaaaataa caatctttgg catattaaac
    21301 ctcgtccacg gaatggcctt tcagcgcacg acgaaccgac tggtccatgc ggcgagcggc
    21361 gaaatcctgg gtttgtttgt ctacgttttt aatcgcttgt tcgatggcgt caacatcatc
    21421 gccctgcgcc acttcactca ggtgagcggc agcatcgtca atgacctgac gctctgcggt
    21481 gcttaacagc gcggcatcag cagccagcgc gccgtgcaaa ctttccagca cacgcgccgc
    21541 ttctactttt tgttctgcca gcatacgggc ttttacgtcc tgctcggcat agctcattga
    21601 gtctttgatc atcgaggcga tttcgctgtc ggtcagaccg taggacggtt tgacctgaat
    21661 agatgcctca acgccggtgg atttctccat cgccgtcacg ctcaaaagac cgtcggcatc
    21721 gacctggaac gtcacgcgaa tatgcgcgcc gccagccggt aacgccggaa taccacgcag
    21781 cgcaaaacgc gccagcgagc ggcagtcctg caccagttcg cgctcaccct gcattacatg
    21841 gatagacatc gccgtctgac catctttaaa ggtggtgaaa tcctgagcgc gcgccaccgg
    21901 aatagtggta ttacgcggaa tcactttctc caccaggccg cccatcgttt cgaggcccag
    21961 cgacagcggg atcacatcca gcaacagcat ttcgctgtcc ggcttgttac ccaccagaat
    22021 atccgcctga atcgccgcgc caatagcgac gactttatcc gggtcgatag aagtcagcgg
    22081 tggacgaccg aaaaattcgc ctactcgttc acgcactagc ggcacgcgag tagaaccgcc
    22141 aaccatcacc acttccagca cttcatcagc ttctacgcca gcgtctttca gcgcgcgacg
    22201 acaagccagc aaggttcgtt ttaccagtgg cgcgatcagt tcgttgaatt gttcacggct
    22261 gatttcgccc tgccagcccg caacattcac agtcacggag tccgcatcgc tcagcgcgat
    22321 tttggctgca atggcggcat ccagcagttc acgctgaacg cggttatcgc tacgatcagg
    22381 aatgcccgcc tgctcgcgaa tgtaatccgc cagcagatgg tcgaaatcat cgccgccgag
    22441 cgcggaatca ccgccggtcg ccagcacttc aaacacgccg cgacttaagc gcagtatgga
    22501 aatatcaaac gtcccgccgc cgaggtcata aacagcgatc acgccttcct gaccggaatc
    22561 cagcccgtag gcgatagccg cagcggtcgg ttcgttaagt aagcgcagaa catgcaaccc
    22621 cgccagacgc gccgcgtctt tggtgccctg acgctgggca tcgtcaaagt acgccggaac
    22681 ggtgataacg acaccatcca gctcgcctgc cagggcttca gttgcccgcg ccgccagtgc
    22741 tttgaggatg tccgcagaaa cgcgcaccgg gttcagcagc cccgccgccg tttcaatcat
    22801 cggcaggccg ttttcgctgg cctggaattg ataaggcaga tgtggatagc gttgctggat
    22861 atcagccagc gagcgcccca tcaggcgttt aacagaacta atggtgttgg cggtatcgag
    22921 cgctgcgtta gtacgcgcat cataacccac cgaatgccct tgctgttgat agtgaacaac
    22981 agacggcagc aggtgacggc cttcatggtc ggctaacgtt tcggcctgac cgctgcgcac
    23041 tgtcgccacc agcgagttgg ttgtgccaag gtcgataccg gccgccagac gacgctgatg
    23101 cggcgcagca ctcaaaccag gttcactaat ttgtaataag gccatgttta gcttccagaa
    23161 attaaaaatc gagcagtttt tcttcgagtt gttcggcact gcttcgcagt ttatcgagaa
    23221 aacgcagctt acgcacggta tccgccgccg cgtcccacgt ctcgttgtct aactgttcaa
    23281 ccatcaactg atggcgggta tcaaacatct ttttcacacg tttgataaag ctttccagcc
    23341 gcgcttcatc tttcgcctgt tcgatctcgt ccagttcttc gcgcagttcc agctgttcca
    23401 tcaggaacgc ggtgtcgcgc acagtatgct gctcgctggc aagatcaaaa ccgtgcaaag
    23461 aaagcaaata ttccgcgcgc attaacggat gacgcagcgt ttgccaggcc tggttaatgg
    23521 tcgcggattg ctgtacggcg gcgagttgtt ccgcctggct tccgctggcg aatttatcag
    23581 gatgatactg acgttgtaga tcctgaaaac gcaggctcag cgcctgggta tcgagttgat
    23641 aacaggcagg caagccaaag agggtgaagt aatccataac attctcaggg ctgcgatccg
    23701 ccgcgataac ggcgaatcag gtaaaacaaa ccccacgcgc aggcgaccac ggtggggtta
    23761 tcggtatgcg catcaaacgt ggaagctttc gccgcaacca cactcatctt tgacgttcgg
    23821 gttggtgaat ttaaaccctt cgttcaggcc ttcttttacg aagtccagct gcgtaccgtc
    23881 cagaaattgc aggcttttgc catcgaccac gactttcacg cctttgtctt caaacacgat
    23941 gtcttccggc gtcggttcgt caacaaattc cagtacataa gccatacctg aacatccgga
    24001 ggttctcacg cccagacgca ggccaaaccc tttgccgcgg ttagccagaa aggtatttac
    24061 tcgcgctgct gcactgtcgc tcagtgtaat cgacataacc aaacctcaac tcttattttg
    24121 cttcacgttt gcttttatag tccgcaatgg cggctttgat cgcgtcttct gccagaatag
    24181 aacagtgaat tttcaccggc ggcagttcaa gttcttcagc aatatcggtg tttttgatcg
    24241 cctgcgcttc gtcgagagac ttccctttca cccattcggt gaccagggag ctggaagcga
    24301 tagcagaacc gcagccgtaa gttttaaaac gcgcgtcttc aatgatacct tcatcgttga
    24361 ctttaatctg caacttcatc acgtcgccac aggccggtgc acccaccatg ccgctgccga
    24421 cgttctcgtc gttgttgtca aaggaaccca cgttacgcgg attctcgtaa tggtcgataa
    24481 ctttttcgct gtaagccatt ataaattctc ctgattccga taccgattaa tgatgagccc
    24541 attcgatgct gttcagatcc acgccctgct tgtacatttc ccacagcgga gaaaggtcac
    24601 gcagacgacc gatggattta cgaactaact cgatggtgta gtcgatctct tcttcagtag
    24661 taaaacgacc taaagagaaa cggatagagc tatgtgccag ctcgtcgttc agccccagcg
    24721 cgcgcagcac gtaggacggt tcgaggcttg ctgacgtaca ggcggaacct gaagaaaccg
    24781 cgaggtcttt cagcgccata atcagcgact caccttcaac gtagttgaag ctgacgttga
    24841 gaatgttcgg cgcaccgtgt tccaggtcac cgttcaggta aacttcttcg atatctttga
    24901 tgccgttcca cagacggtta cgcaggccgc gcagacgttc catctcggtc gccatctctt
    24961 cttttgcgat gcgataggct tcgcccatac cgacgatctg gtgaacaggc agagtgccgg
    25021 aacgcatacc gcgctcgtga ccgccgccat gcatttgtgc ttcgatacgt acgcgcggtt
    25081 tacgacgcac atacagcgca ccaatacctt tcgggccata gattttgtga ccggagaaag
    25141 acatcaggtc aactttcaac tggctcagat cgataggcag tttgcccacg ctctgggttg
    25201 catcaacgtg atagataatg ccacgagcac ggcacatttc gccgatagcc gcgatatcct
    25261 gcaccacgcc gatttcgtta tttacgtgca tgatggaaac aaggatggtg tcgtcacgca
    25321 tcgctgcttc gagttctttc aggtcgataa tgccattacg ctgcggtgcc aggtaagtga
    25381 cttcaaaacc ttcgcgctcc agctgacggc aggtatccag taccgcttta tgttcggttt
    25441 tactggtgat gatgtgcttg ccttttttct gataaaagtt ggctgcacct ttgatcgcca
    25501 ggttgtcaga ttcggttgca ccagaggtaa agacgatttc acgcgggtca gcgccgacca
    25561 gatcggcaat ctgattacgg gcgatatcaa ccgcttcttc agcctgccag ccgaaacggt
    25621 gagaacggga ggccgggtta ccaaaggttc cgtccatcgt cataaactgc atcattttct
    25681 cggcaacacg cgggtccacc ggcgtggttg cggagtagtc gagataaatc ggtaatttca
    25741 ttgctctata aactccgtac atcactcaat gcaaggaacc aggctaccgg ctggatgtac
    25801 gaccgtgttt acggagtgtt tagcactccg gcctgattct gaattctttt tattaagcgc
    25861 gtaacttaac gtcgatcgcg tcttgtgtgc gggtgcgtgg cgcgtcgtga gtatgctgac
    25921 gaccagacac atccagcact tcctggttat taaccagttc gcctaaagta atgttattga
    25981 gaaaaccggt aagacggtcg ctcaaatcac gccacagcgc gtgggtcagg catttatcgc
    26041 cgccctggca gccgccttta ccctgacaac gggtggcatc tacagattcg tcaacggcgc
    26101 taatcacttc gccaacggcg atgctgctgg catctttgcc taacagataa ccaccgcctg
    26161 gtccacgtac gctggaaacc agaccatttt tacgcagacg ggaaaacagt tgttccagat
    26221 aagaaaggga aattccctga cgttcggaaa tatcagccaa cggtaccggg cccgcttcag
    26281 agttgagcgc aacgtcaagc attgcggtca cggcatagcg ccctttagat gtcagtctca
    26341 tgtcttactt cacctcaaac tcgcccctgc ccggggtttt ttattgtaaa agtgggggta
    26401 ttgcatagca gggtcaagtc tgacattccc gagtaaattg gtcaactatt tacttgactg
    26461 atttagtcgg gtatttaacc ttcagtgcca ttttttatcg tggcgtttgt acctgttgtc
    26521 ggatgcggtg tgaacacctt atccgacctg ggattccgtg cacttacagg cctgataaga
    26581 cgcgttaagc atcgcatcag gcaaccactg ttattcggcc ttgttacctt tattctgctg
    26641 ctcaatagaa gccagaatcc cgcgcaggat attcaattcc tggctttccg ggcgtgcacg
    26701 ggtaaacaga cgacgcaatt tattcatcac ctgccccgga tggttttcac gaataaaacc
    26761 ggttgccagc agcgtttgtt ccagatgacc ataaaaacgc tccagatcat cgaccagcgg
    26821 atacggcgtc tcttcatgct cggcctgctc accattttcc tgagttgcca gccaggccat
    26881 gcgcacttca taggcaataa cctgaaccgc catcgccaga ttcagcgaac tgtattccgg
    26941 gttagcggca atcgcgacat gataatggca tttctgcaac tcttcattgg tcaagccgac
    27001 gcgctcgcga ccaaaaacca gcgccaccgg ggtatttgcc gcttcagcga cgcttttcag
    27061 accgcattcg cgcgggtcga gcatcggcca cggcagcgtg cgggagcgtg cgctggtgcc
    27121 caccaccagg ctacaaccgg ctaacgcttc gtcgagcgta tcgacgatgt gagcattacc
    27181 aatcacatcg ctggcccctg ctgccagggc aatcgcctgg gagtcaggtt tcaccagtgg
    27241 attaaccagc cacagattgg ttaatcccat tgttttcatg gcacgggcaa cagaccccat
    27301 attgccggtg tgtgacgtct ccaccagcac aattcgaata ttttgcagca ttgtctttct
    27361 tcatctaaag attattcacg catcttatca taaaacgaag acagatgccg atctcgctgc
    27421 tatactctgc gccgttttcc cgttctttaa catccagtga gagagaccga tgcatccgat
    27481 gctgaacatc gccgtgcgcg cagcgcgcaa ggcgggtaat ttaattgcca aaaactatga
    27541 aaccccggac gctgtagaag cgagccagaa aggcagtaac gacttcgtga ccaacgtaga
    27601 taaagctgcc gaagcggtga ttatcgacac gattcgtaaa tcttacccac agcacaccat
    27661 catcaccgaa gaaagcggtg aacttgaagg tactgatcag gatgttcaat gggttatcga
    27721 tccactggat ggcactacca actttatcaa acgtctgccg cacttcgcgg tatctatcgc
    27781 cgttcgtatc aaaggccgca ctgaagttgc tgtggtttac gatcctatgc gtaacgaact
    27841 gttcaccgcc actcgcggtc agggcgcaca gctgaacggc taccgtctgc gcggcagcac
    27901 cgctcgcgat ctcgacggta ctattctggc gaccggcttc ccgttcaaag caaaacagta
    27961 cgccaccacc tacatcaaca tcgtcggcaa actgttcaac gaatgtgcag acttccgtcg
    28021 taccggttct gcggcgctgg atctggctta cgttgctgcg ggtcgcgttg acggtttctt
    28081 tgaaatcggt ctgcgtccgt gggatttcgc ggcaggcgag ctgctggttc gtgaagcggg
    28141 cggcatcgtc agcgacttca ccggtggtca taactacatg ctgaccggta acatcgttgc
    28201 tggtaacccg cgcgttgtta aagccatgct ggcgaacatg cgtgacgagt taagcgacgc
    28261 cctgaagcgt taatgactct ggcgggtgat gtcactcacc cgccctcgcc tttcaggcgc
    28321 tattccgaaa tacttcctca ccgctttact ttctttcgcc actctcccac catctttccc
    28381 cgattaatgg atgataaaga agtcgcaagg aaaggttatg gcgctgccag tgaacaaacg
    28441 cgttctcaaa attctgttta ttctctttgt tgttgccttc tgcatttatt taataccgcg
    28501 catcgccatc aattttttct attaccctga cgacaaaatc tacggtcccg aaccctggtc
    28561 ggcggaatcc gtcgaattta cggctaagga cggtactcat ctgcaaggct ggtttatccc
    28621 ttcttcgacg ggccctgctg ataacgccat tgctaccatt attcatgctc acggcaatgc
    28681 cggaaatatg tccgcccact ggccgctggt cagttggtta cccgagcgta atttcaacgt
    28741 ttttatgttt gattatcgcg ggtttggtaa atcaaaaggc acgccgtccc aggccggatt
    28801 gctggacgat acgcaaagtg ccatcaatgt ggtgcgccat cgcagtgatg taaacccaca
    28861 acggctggtg ctgttcgggc agagtattgg cggggcgaat attctggatg ttattggtca
    28921 gggtgatcgt gaaggcatac gtgcggtgat cctcgactcc acatttgcct cttatgcgac
    28981 catcgccaac cagatgatcc ccggcagtgg ctacttactt gatgagagtt acagcggcga
    29041 aaattacatc gccagcgtca gcccgatccc gcttttactg attcacggta aagctgatca
    29101 cgttatccca tggcagcaca gcgaaaagtt gtatagcctg gcaaaagagc caaaacggtt
    29161 gatcctaatc ccggatggcg aacacattga tgctttttcc gatcgtcacg gcgatgttta
    29221 tcgcgaacag atggtggatt ttatccttag cgcgttgaat ccgcagaact aacccatgat
    29281 cgctagcacg ataatcattc acaaaactac cttaagacat gctaatccac tggtcagaac
    29341 agtttaagat gagaaaaatt ctgtgacgct tgccaacatt tctgatgatt agcattccct
    29401 tcgccatttc cctgagcaaa ctttagctat tcttatcaat tatgcttatg ggagatctac
    29461 agatgatgcc tacgcttgct ccaccatctg tcctttcggc tccccagcgc cgctgccaga
    29521 tcttgctgac gctctttcag ccggggttga ccgccaccac ggcaactttc agcgagctta
    29581 atggtgtgga tgatgatatt gccagtcttg atatcagcga aacaggacgg gagatcctgc
    29641 gctatcatca actcacactg actactggtt atgacggtag ctaccgggtt gaaggtacag
    29701 tgcttaacca acgtttgtgt ttatttcact ggctacgacg aggtttccgt ctgtgtccgt
    29761 catttattac cagccatttc acccccgccc tgaagagtga actgaagcgg cgtggaattg
    29821 cgcgtaactt ttacgacgat accaatctac aagcgttagt gaatctctgc tctcgacggc
    29881 tgcaaaaacg ctttgaaacg cgcgatattc atttcctgtg tctgtatctg caatattgtt
    29941 tgctgcaaca ccacgctgga attactcctc agtttaatcc gctccaacgt cgctgggcgg
    30001 aatcctgcct tgaatttcag gtagcgcagg aaattggacg ccactggcag cgtcgagcgc
    30061 tccagcctgt accgcctgat gagccactgt tcatggcatt acttttttcc atgttgcggg
    30121 ttcccgatcc actgcgggat gcgcatcagc gggacagaca actgcgtcag tctatcaaac
    30181 gtctggtaaa ccattttcgt gagctgggaa atgttcgttt ttatgatgaa caggggttgt
    30241 gcgatcagct ttatacccac ctcgcccagg cgttaaatcg cagtttgttt gccatcggta
    30301 ttgataacac cctgccggaa gagttcgccc gcctgtatcc acgcctggta cgcaccaccc
    30361 gcgcggcgct ggccggattt gaaagtgaat acggcgtcca tctttctgat gaggaaagcg
    30421 gtctggtcgc ggtgattttt ggtgcctggc taatgcagga aaacgacctg catgagaaac
    30481 agattattct actgaccggg aatgatagcg agcgagaagc gcagattgag cagcagctac
    30541 gcgaactaac gttactgccg ctcaacatta aacatatgtc ggtaaaggta tttttgcaga
    30601 caggcgctcc gcgcggcgcg gcactgatta ttgctcctta taccatgccg ttaccgctct
    30661 tttcaccacc gctgatctat acggacctga cgctgacaac acatcaacag gagcagatcc
    30721 gcaaaatgct cgaatcagca tgagggaaca actttcgggc gcaaaaacat tgccggaagc
    30781 gccaccagcg ccatcaccca gaacacgccg tgccccagat attgatacag gaaaccggcg
    30841 aaaacggtca tgatcgcgat actgccgccc atcgcgacgg cagagtaaac cgcctgtaaa
    30901 cggatgactt cgctaccctg gcgagcggca atatagcgca tggcggccag gtggcagacc
    30961 gtgaaggtgc cgcaatgcag aatttgcacc actatcaacc acggcatcgc cgtcgttgct
    31021 cccataatgc cccagcgcac tacgccgcaa atcgccgaga tcaacagcat atcgcgtgca
    31081 ctacaacggc ggaaaagttt attactcagc gcaaagataa tgacttccgc caccacgccc
    31141 agcgaccaca aatagcccac cgccgaggcc gagtagccag cggcctgcca gtaaatggcg
    31201 ctaaaaccgt aataggccgc atgcgccccc tgcaataaac aaacgcaggc cagaaagcgc
    31261 cagttctggc gaaccagcgc caaccacgca gaccaaccgg tgctctcctg ctggcggctt
    31321 gccccttgtg gctgaatcgt cggacggatg agaaagccga gcagcatgga tgccacgccc
    31381 aacgttaaca gcgcgaggat cacccgataa tcaaacatag tgaccagttt acccgtcaac
    31441 gccgagccaa tgacaaacgc caccgagccc cacaatcgta ctttgccgta atcaagcggg
    31501 aactgctttt gccacgtatt cgccagtgca tcggtcaacg gtaccagcgg tgagaaaaag
    31561 aggttaaagc caatcatcac cagcatcagc cacgctacgt gcgcccccgc ccagaaggcg
    31621 acagcaaaga gaagtgtcag cagtgccagc acgcgcaagg cggaaatcag gcgggaagga
    31681 tcgctgacgc ggggcgcgat gagcaaactc ccgagaaaac gggcaaccag acctgccccc
    31741 aataacaggc cgatggtttc tggcgttaaa ccaatcccct taagccagac gctccagaaa
    31801 ggtagaaaaa tgccgtaact aaaaaagtat gtgaaatagc cgagcgccaa ccagcgcgtg
    31861 gattgcaaaa ccatgagtcc ctcccgtcag gaggcgttag tctggggtta atcgaatgtt
    31921 ggtgcaagtt gaaaaataag aatattcgag cctgcttcct gacggttatc ctgcatatca
    31981 ggaaatgaca aaaaagagcg tcccacaaca caacatgtaa aaaagaacga tgaaacaggt
    32041 gaaaatcgca atttattgaa ctgacaaact catgtgagac ggataaggcg tgcatgtcgc
    32101 atccggcagg aaacaataag cacattgtca gccatctgcg ttatgccgtt ataccagaaa
    32161 cgaatatctc aacgcctggg cgtggcgagg taccagagtg tgtattacgt ttaaatcaca
    32221 tcatcttgca aagggattgg ttatgaactc actacgttat tttgattttg gcactgcccg
    32281 ccccgtttta ttattaattg cccgtatcgc cgtggtctta attttcatta tttttggttt
    32341 tcccaaaatg atgggctttg acggtacggt ccaatatatg gcctcgctgg gcgcaccaat
    32401 gccgatgctg gcagcgatta ttgcggtagt tatggaagtg cccgccgcga tattaatcgt
    32461 gcttggcttt ttcgcccgtc cgctggcggt gctgtttatt ttctacacgc tgggtacggc
    32521 ggtgattggc caccattact gggatatgac cggcgatgcg gttgggccaa atatgattaa
    32581 tttctggaag aatgtcagta tcgctggcgc attcctgctg ttggcaatta ccgggccggg
    32641 gacgatttct ctcgatcgcc gttaggaaag atgccggatg cggcgtaaac gccttatccg
    32701 gcctaccgct ccaacaccga ttgtaggcct gataagacgc agcaagcgtc gcatcaggca
    32761 tcgtgcaccg agtgctacaa aatcgaaatt accccgtagg cctgtgcatc cggcatttta
    32821 taaattacag caacgcaacc cgcaacgaca tctctgaaat tgttgattcc cctggtgcaa
    32881 gcgcaatgag gtcaccacct tccggacgat gatggtcatc cggcgcatgg ctcatcggtt
    32941 ccagacagaa gaaatcaaac gcatatcctt tatcaaacgc agggtctgaa acaaagatga
    33001 aataacacgg tgcaggtggc gtcgtttcca tgatgatggc ataaccctcc tgcggctgct
    33061 cgatgcgggc cttaccattc catccggcaa aaccattgtt tacccactgg cgcggcaacg
    33121 gcgcgagctg gttaaaatcc agctcctgcg gtagctgctc gcaaaactca cccgccagcc
    33181 actgctcccg ctccagccag taaccgctcg cctgtgcctg aatccgcgtt tgcggcgaca
    33241 acggaaaata aggatgccag ccggtaccaa atggcagcgt ctctgcccct ttattggtga
    33301 cagagagcgt caccgtcagc gtatctgccg ttaaatgaaa cgcctgactt acccgatagt
    33361 gataaacacc gctgcgatgt tcatacacca gacacaagct atcttcgctg cgcgagacac
    33421 attgccactg ccccagccag gcatcaccgt gtagatagtg cgcatcccac tcaacattcg
    33481 gttgtagttg atattcacgt ccctgccaga caaaacgatt accgctcacc cgattagcaa
    33541 acggaaccag cggaaaacag gaagcgtcgg tagcaacgcc gctttttttc cccggacgca
    33601 gcagcggggc ggcgtcacgc cagaagcctt caattacacc gccctgatcg gaaacctcaa
    33661 gctttagcgc cccctgagat agggtataga tggtcatcct ctactcctta atccgggaag
    33721 ttgatcacga ctttcccgca tttgccgctc gccattagcg cgtaagcatc tcccgcctgc
    33781 tccagtgaga agcgatgggt aatagcgtta cgcggccaca gtttccagtc agtcaaatcg
    33841 tgggcgcatt tttccatatg gaacaagctg gtcacccagg agccaataat tctgcgttgg
    33901 tgatgcatca ggtcggcgct aacctcgaac tcgacttttc cggtttcacc aatgtaaacc
    33961 acgcgtcccc agtcagcggt ggattgcagt gccagtaagc gacctgcggc attaccggaa
    34021 caatcaagcg caacatccgc gccgccgtga gtgagttcgg cgattatctg cggcatacct
    34081 tcggtggtcg ctaaatagcc gtgatccatc actcctaact gtttcgccat cgccagacgt
    34141 tccggcaaca tatcaacgcc gatgatccgt tttgcaccgc gacctttcgc cagcatcatc
    34201 gccatcatgc cgaccggccc cagaccaacc accaccacgt tatcgctacc ggaaacctcg
    34261 ccgcgcaaaa tcccttcata cgccgtacca acaccgcaac tgataaacgc gccatcttcg
    34321 taactcagcg cgtctggcag gaggatcaga tctttttctt ccgccagtag gtattcggca
    34381 tgaccgccgt cacgctgcca gccgtaagcc gcttttcctt cgccagtaca agaaatagga
    34441 aaaccgcgac ggcagttcgg gcaaaaacca cagccagaaa tgtgatacac cagcacgcgg
    34501 tcgccctctt taaaatggcg acagccttgc cccatcgcca caatctgccc gcacggttca
    34561 tgaccgttga taaatccctg gtataacggt ttatcgggtg ccgccgccgt agcgcggtgc
    34621 tggtggtaga tatagtggac atcgcttccg caaatcccgg aggatttcat tttgatcagt
    34681 acctggttaa tccccggcgt cggcaccgca acttcccgca gatcgacggt cgaatttcct
    34741 ggtaaataag ctgccagcat cgttttcata aatccctcat taacaatacg attaattttc
    34801 atccctgccg cacccgcgcc aggggcgtta aggtttagcg tttcgcttta ctgctacgct
    34861 gggtcagcaa gatattcgcc agcaccgcca ccacgatgat gacgccgcgc accacctgct
    34921 ggaaaaagga gttaatcccg agcagcacca gaccgttacc gattagcgta atcaccagta
    34981 cgccaagcag cgtaccgaac aacgagccgc gaccgccgga aagcgccgta ccgccgacca
    35041 cgaccgcggc gatgacgtca aactccagac cgtttgcggc acctgcgtta ccggaaccga
    35101 ggcgggccgc caacaaaatg ccggtcaccg ccgctaataa tcctgaaagg gtaaaaataa
    35161 gaatgcgtac ccgacgaacg ttaataccgc acaactgcgc tgcagtggca ttaccgccaa
    35221 cagcaaaaac cgagcgcccg aaggcagttt tgcggctaat gaacacaaac agcgcaaata
    35281 acaccatcat gatcagcgcg gataccggca ccccgagaaa ttgtccgccc agccagtcca
    35341 gcacttcgtt ttcgttaatt ggcactggca aggcgttcgt cataaacagc cccattccgc
    35401 gcagggcgct ccataaaccc agcgtggcaa caaaacttgg cacgttaaac acgccgcgca
    35461 gcaccccggc gagcgttccc atcagcgcac ctaacagcaa caccagcaga caagcaatcg
    35521 ccagcggaac gtcaaattgc agcaaaaacg ccaggcacac tgaaacaaaa gccaccatcg
    35581 gcccaacgct gacatcaatt tcaccggaga taataatcag cgtcatcgcc caggcggcga
    35641 tcccaatggt ggcggcatcg cgcagcacgt tcatctggtt attcaatgag ataaagccag
    35701 gcgcattcag ggagaagacc agataaagaa tggcaatcac caccagcaaa ccgatctcat
    35761 taatatggcg actgacaaat tgtttgagcg agacgctctt gccctgcggc aatggtaatg
    35821 acgaagcaga catcttcagg ttcctcggta aaattcatca gtgcacagac agaatggcgg
    35881 acatcagctc atccacattg accggagagt gaaactcctg cgagaacgtg ccgtgctgta
    35941 ataacaggat gcggtcacac accagcggca actcctccac ttcactggag ataaacacca
    36001 cgctttttcc ttcggcagcc agctcgcgga caatacggta aatctgctgt ttggcttcga
    36061 tatcgacgcc gcgcgttggc tcgtcgagca acaaaatctg gctggcggca tagacccaac
    36121 gaccgatcac cactttttgc tgattgccgc cagaaagcgt gccgatgggc gtttcgctac
    36181 tggcggcctt gaccgtcatc cgctgcatca cctcttcggt caggcgacgg atggtggacc
    36241 attgcagtac gccgttggcg ctgatttttt gccgattggt cagcactgta ttttcgtcaa
    36301 cgcccaacca gggaatgatc cccgcttctt tgcggttttc tggcgtatag ccaatgccgc
    36361 gtttcagcat gtcgccgtaa tcggggcgcg tgattttctc gccgttgata acaatttcgc
    36421 cctgttcata cgtctccagc ccaacaatcg ccttcagcaa ttcactgcgc cctgccccca
    36481 gcagaccagc aatgccgagc acttcgccac gacgtagcgt aaaactgata tcctccagct
    36541 tgggcttatg gcgtaacgca cggacttcca gcacggcttg atccataatt tcctgagggg
    36601 caaccggcgc aatatcaacg tgatcgcgcc cgagcattag cgacacaata tgatgcgtag
    36661 aggtgttttc gagcatcaca tcgcccgcca cctgaccatc gcgcataacg gtggcacagg
    36721 aggcaatgcg gcgaatttct tccatccggt ggctgacata aatcaccgcc acgcccagtg
    36781 ccgacatctt tttcaccgcg ctgatcacca gttcaacttc cgcactggca agcgagctgg
    36841 tgggttcatc aagaatgacc acgcgcggct cgcccttcat cacccgcgca atttccacca
    36901 gctgcttttg cgccgggctt agtgttgaaa caagttgttc aggactaacg tcaacgccca
    36961 acgcctgcaa gcaacgttgg gcgtcctgcg ccatttgcag gtaatcaatc atgccgttgc
    37021 ggcggggcca ctgaccgagg cagaggtttt ccgccactgt cagcccttcc accagactta
    37081 actcctgata aaccgcacga acccccagtt cagcggcacg gcgagtcagc gtagcttcgt
    37141 caccttccag tcgcgtctcg ccaatccaga tatcaccgct atccggacgt tcgctaccgg
    37201 taagcattcg aatgagggtc gattttcccg caccgttttt gcctaacagc gcgcgaactt
    37261 cgcctttatt gagcgtgaag ttaacgttat ccagcgcaac gacgccgggg taacgcttat
    37321 ttcctgccac cacttttgct accgggactg cctctgttgc cgtgaacata gggacctctg
    37381 cgaatcagcg attagggcag accatcaacg tgcgttgcca gccactgttt accgtcttcc
    37441 gttttggtat agagatcgat tggcacctga atcacttttt caccgtcggc ttgtttattg
    37501 ataaccttca atgtttgcgt aaaaacagca ttgcccattt tcttaccgga aatatccacc
    37561 accgctttca gcacctggtt gttttccagc tcctgagcaa tttcagtggt catatcagaa
    37621 ccgaaaacag caatttttcc ggcctgattt tgattacgta ccgcttttac cgcgccgagt
    37681 gtcgctccgc ccgattcccc cataatggcg ttgagatccg gcgtggagat aatcagtttt
    37741 tcaccgacag aaatcgcttt atctaaaaca gtcccttcct gattagcgac aatttgcgcg
    37801 ccgggaacgc gggcttttaa tacttcttca aaccctttac gtcgttgcac acaaacttca
    37861 aaggcttcgc aattgatgac ggcaattttc ggctggtcaa ttttgttggc aataaaataa
    37921 tcggcggcag cgttacccag ttttttacca aattccagcg ggtcaccaac cagatatgcc
    37981 gagacatatt tatcgacacc cttttgatta atacaggtgt tgtagcaaat cactggaatg
    38041 cccgcctcac tggcgcgacg aacggtgcgg ctgctgccat tttcagaaac agctgacaaa
    38101 ataatggcat cgacattacg cgccacgagg gtatcaacaa aggtactttc tttcgaaata
    38161 tcaccctggg cgttagtttc aattaactgt acctgtactg aagaatcttt tgccgcatcc
    38221 tgaacgccct gacgcactcc ggcgtaatat ccctgggtat caaggtatat tgcgccaata
    38281 gtcatttctt tatcagaagc cctggcaaat agtgcgctac ccaatagcgt agccattaat
    38341 aataaattac gggttcttgt cattttttta ggcatagtgc ctcctgtagg gtttttattt
    38401 acaacggctt attttaatta tttgagcgtg agcggcagcg cgtgccgctc aaatattaca
    38461 gttaataggg tttagtgaac gtagctaaaa ataaaaggca tcacggtggt gaataatgcc
    38521 gctttcggcc aggctggatt aatcgccatc aattcagcgc gagcagattc atagtgcgca
    38581 acctccccta atcccgcggc cgccagcatc gccaccatca ggcatttttc tttatgctgc
    38641 tgttgtaaat cgccatacag cgacaacaag tcaggctgcg agacggcaaa gaaatccgct
    38701 tcgatactgg ttttcgccat ctcttgtgcc cactgtttca tttcactaaa cagttgctgt
    38761 gcggtgtgtt gttcgcccag cagtcgcagc gccattcctt gccagaagag ataatcaacc
    38821 ggctgatcgt tgtaataact gtggatgtta atggtgcgat cgccggtcgc cgccagacgt
    38881 aaacaacgcg tcgcttcagt ttcatcgccc tgcgcgttgg cgcatactgc ctgccagaac
    38941 cagatgtcgt tgtcagtttg ccccggtaaa cggccttcgc ttaaattctc cggataatgc
    39001 agcgcggcat gaagcagttc gctggcctgc tgcggctctc tggcatcaag atgctgccag
    39061 gcgcgtaata actggttgag gataaactgg ctggtgacct tcccttcccc accttcccac
    39121 gggtggaatt tgcgcgtggc gagaatgtcc gccgctttgt ctgcctgacc agtgagatgc
    39181 cacaaattga gcagttctgc ggtcatgtcg tcgcgtttca gcgcaattcc cagattattt
    39241 tccagacgcg ccagtcgttt ctccggtgtg acaccgctta acttatccag caaatcccgt
    39301 tcgaaaagca gacgtgcatc ctgcggcgca agctgataag cattatcaag ataacgcgcg
    39361 gccagctcat aatcgtgctg cttattccac gcatggatcg ccaacccgcg ccagccgtcg
    39421 gcaaactccg gcgacatctc tacgcaacgc tgccataagg caatggcttt gccgtagcta
    39481 cgcttgttgt agtagaagca ggccagtaaa tgccgggcaa accagcactc ttcgatactc
    39541 tccagcgccg ccacttcttc cagcgtattc gggaaacgga caaactgcgg gaaggcatca
    39601 atagctttta cgaccagttc tccgcgttca gcttttggta gcaaactggc ttgcaggtaa
    39661 agcggtagcg tgcgctgaca gtccagtgcg ttcagcatct ctgccgccag ggcgggcatg
    39721 ccccagttaa tcagttgccc ggcggtcatc agggcgttaa cgtcgcgtcc ctgacaaaga
    39781 cctcgccact gcgcgagggc tgattcgcta cgaccatcga accagttcag ccaccacaga
    39841 gtggcgttca gcggataatc acgcagcagt ttctcgcgct gcaaacgcgc gttgtcctga
    39901 cgaccactta acaccagtag caggttatgc aggcaaagca cttcctgatt ggttgggcag
    39961 gcgcgaagac tttgttggca aaaatccaga ccagtgtcga agttaccatt acgcgccgcc
    40021 agtcgtgcca gaccataata gccaccggct ttactgttgc cgctccagac cgcacgccag
    40081 aaatcctctt cggcttgttg atattgcccc tgacgttcgt aagcactggc gcgaatcaaa
    40141 ctcgcctgtc cgcactgcgg atttttgttc agcgcatgtg cgcgtttcag tgcctgactg
    40201 gcatatacca ccgcttgcgg gaaatcggca cggttatatt ccagcattgc cagcgccagg
    40261 ttacagcgat aatccagcgg atccagcgcc acgccgcgca ggtagtaatc gaacggtgaa
    40321 cggctcgcgt gatgatattg ctccagatgt tgaccgataa accaggcttc atctgtactg
    40381 gtaatgtctt gtgctgccag tggcgctttg gcgacgtccg gcaacggcaa cgcttgcgac
    40441 tgatgttcat gataactcag tacaatattg ccgtcggcat cggagagctc aatggtcagc
    40501 ctttcaggat tgataccgtg caacacgccc tggatggcgg tcgctggtgt cagggccacg
    40561 gcatcatcga gcaacgcgtt gcatttgccg atttcgcgga tcgccaggcg gtatccgttc
    40621 aacggagaga tggcatacag cccccactca atcccccgct cactacgctg gagttttatc
    40681 accgcatcgc gggaggcatt ttgcaccatg cccagagaat gataaggcag gaaatactgc
    40741 tcgaaacgct tctcctcgta agcatcaagc caggtaaaat caggctggtt atcggcaaaa
    40801 ataccggtca tcagttcgat atacgggccg ttattgtcag tcaggctctt atcccacgcc
    40861 tggccaaatt cactgtgtcc ccagctccac tgttttttac ctggcgcaat atggtggttg
    40921 gcaacgtgta gcaaaccacc atcttcatcg tgacaccacg cgccaacaaa atcgtactgt
    40981 gatttttcag ccatatatga ggttggaacg ggcacatttt tatagcgaga aatgtccact
    41041 ccggcggagt agtccacttt gtagtaagtg ccggtggcga tggggaaagc ggagacggcc
    41101 cgtttgccgt gatcaaacac cgcagtcaca tccggtggga aaacgctctg atgcccttca
    41161 ccccctttca ctgccgggtt ggcccaccac aagaaatgac gcggcgtggc gttgccgtta
    41221 tagactcggc tggcgatttc cagcgccgcc cggtcagggc gcagggtgaa acctgtcatc
    41281 acctgtaaac cgtgcatcgg ctccgtttcg ccgacccaca ccgtctgtgc gccgtcgtca
    41341 tgggcttcga gggtgaaatc aacgggcata aaggtagtcg ggcgatggtg ttgcggccag
    41401 ttaaactcaa tcccgccgga gatccacggc cccagcagtc ccaccagcgc cggtttaatg
    41461 acttcattgt gataaacaaa atcgcgctgt ttcactttat cccacgcgcg atgcacccga
    41521 ccgcccagtt ccggcaggat catcactttg atgtagtcgt tttccagcca caccgcctgc
    41581 caggatttca gggttttctg ctcgctcagc gtatcggtca cgccgtaggg ataaaccgcg
    41641 ccggacgatc cctgataaac gcgattttcc aggaacatgg gatgtatatc ctgcggcccg
    41701 gtttcatagg tcgggatctc aacgcgctct tgccacactt ttactggagt catggtgccc
    41761 tcaatattaa caagacatac tgaattaaaa gatttgtggc agtgtattga acaatctggc
    41821 aatgttttcg cggaataatc acgcaattaa ctaaacaagg tttagtgaag atgagagcct
    41881 gcattaacaa tcaacagatt cgccaccata acaaatgcgt gattctggaa ctgctgtacc
    41941 ggcaaaagcg cgccaataaa tcaacgctgg cccggctggc gcaaatttcg attccggcgg
    42001 tcagtaatat tttgcaggaa ctggaaagcg aaaaacgggt ggtgaatatc gacgatgaaa
    42061 gccagacgcg cgggcatagt agcggtacat ggctgattgc gccggaagga gactggacgc
    42121 tgtgcctgaa cgtgacgccc accagcattg agtgtcaggt cgctaatgcc tgtttaagtc
    42181 cgaaaggcga atttgaacgc tttcagattg acgcaccaac gccgcaggcg ctgctgtccg
    42241 aaatcgaaaa atgctggcat cgccaccgta aattgtggcc ggaccgcacc atcaatctgg
    42301 ctctggcaat ccacggtcag gtggatccgg tgaccggtgt gtcgcaaacc atgccgcaag
    42361 cgccgtgggc aacaccggtt gaggtgaaat atctgctgga agagaagctg ggcattcggg
    42421 tgatggtcga taatgactgc gtgatgctgg cgctggcgga gaaatggcaa aataattcgc
    42481 aggaacggga tttctgcgta atcacgttga ttacggcatt ggctcgtcgt tcgtgattaa
    42541 cgagcaaatt tatcgcggca gtttgtatgg tagcgggcaa attggccaca ccatcgttaa
    42601 tccggatggc gtcgtctgcg actgcgggcg ttatggctgc ctggaaaccg tcgcctcgtt
    42661 aagcgcatta aaaaaacagg cgcgggtatg gctaaaatca caaccggtta atactcagct
    42721 tgatcctgaa aaactgacta ccgcgcagtt aatcgctgcc tggcagagtg gagaaccgtg
    42781 gatcaccagt tgggttgatc gctctgccaa cgccattggt ttgagtctgt ataacttcct
    42841 caacatcctc aatattaatc agatttggtt gtacggtcgc agttgtgcct ttggtgagaa
    42901 ctggcttaat accattattc gccagacggg atttaacccg ttcgaccgcg acgaaggacc
    42961 gagcgtgaaa gcgacgcaaa ttggctttgg gcaattaagc cgcgcacagc aggtgctggg
    43021 aattggctat ttgtatgttg aggcgcagtt acggcagatt tgatggcgca ataacgtaga
    43081 aaggcttccc gaaggaagcc ttttttcaac agattaaccg tttcgcttat gcgtaaaccg
    43141 gataacgtgc gcagatgtcg agaactttac ctttgatgcg ctcgataaca gcttcatcat
    43201 tgatgctgtc cagcacgtca cacatccagc cagccagttc tttcgcttcg gcttctttaa
    43261 agccacgacg ggtaatcgcc ggagtaccta cacgaatacc ggaggtcaca aacgggctct
    43321 tcggatcgtt cggtacgctg tttttgttga cggtgatgtt agcacggccc agggcggcgt
    43381 cagcttcttt accggtcagg tttttatcaa ccaaatcaac caggaacagg tggttatcag
    43441 tgccgccgga aaccactttg tagccgcgtt ccaggaacac ttctaccatc gctttagcgt
    43501 ttttcgcaac ctgctgttgg taagttttga actcaggctc catcgcttct ttcagagcaa
    43561 ccgctttacc agcgattacg tgcatcaacg ggccgccctg accaccaggg aaaacggcag
    43621 agttcagttt tttgtacagc tcttcgctac cacctttcgc caggatcagg ccgccgcgcg
    43681 gacccgccag ggttttgtga gtggtggtag taacaacgtg agcatgagga accgggttcg
    43741 ggtagacgcc agcagcaacc aggcccgcaa cgtgcgccat atcaacgaac aggtaagcac
    43801 cgatgctgtc agcgatttca cgcattttcg cccagtcaac cacgccggaa tatgcagaga
    43861 agccaccgat aatcattttc ggtttgtgtt ctttggcttg tttttccaga tcggcgtagt
    43921 cgatatgacc ggtagcatcg ataccgtaag gaacgatgtt gtacagttta ccggagaagt
    43981 taaccggaga accgtgagtc aggtgaccgc catgcgccag attcataccc agaacggtat
    44041 cgcctggttc cagcaacgcg gtgtagaccg caaagttagc ctgggagccg gagtgcggct
    44101 ggacgttagc gtagtcagcg ccgaacagtt ctttcgcacg atcgatcgcc agttgttcaa
    44161 cgatatcaac atactcgcaa ccgccgtagt agcgtttgcc cggataacct tcagcatatt
    44221 tgttggtcag ctgagaaccc tgcgcctgca ttacgcgcgg gctggtgtag ttttcggagg
    44281 cgatcagttc gatgtgctct tcctgacgta ctttttcctg ctccatagcc tgccacagtt
    44341 cggcatcata atcggcaatg ttcatttcac gctttaacat ccgcatctcc tgactcagct
    44401 aacaataaaa tttttggcct ttataggcgg tcctgttgga caacggcgaa cagtataacc
    44461 gaatcattgt gcgataacag gtcttgacaa aggaatttac gcaaacgatt accttcaggc
    44521 taagcaaggc tttggagaat aaagagcttg caaccggaaa cggatttctt ttcaggtttg
    44581 tgatgcaaat ttttcacttc atcacattct ttctgaaaaa caccaaagaa ccatttacat
    44641 tgcagggcta ttttttataa gatgcatttg agatacatca attaagatgc aaaaaaagga
    44701 agaccatatg cttgacgctc aaaccatcgc tacagtaaaa gccaccatcc ctttactggt
    44761 ggaaacggga ccaaagttaa ccgcccattt ctacgaccgt atgtttactc ataacccaga
    44821 actcaaagaa atttttaaca tgagtaacca gcgtaatggc gatcaacgtg aagccctgtt
    44881 taacgctatt gccgcctacg ccagcaatat tgaaaacctg cctgcgctgc tgccagcggt
    44941 agaaaaaatt gcgcagaagc acaccagttt ccagatcaaa ccggaacagt acaacatcgt
    45001 cggtgaacac ctgttggcaa cgctggacga aatgttcagc ccggggcagg aagtgctgga
    45061 cgcgtggggt aaagcctatg gcgtactggc taatgtattt atcaatcgcg aggcggaaat
    45121 ctataacgaa aacgccagca aagccggtgg ttgggaaggt actcgcgatt tccgcattgt
    45181 ggctaaaaca ccacgcagcg cacttatcac cagcttcgaa ctggagccgg tcgacggtgg
    45241 cgcagtggca gaataccgtc cggggcaata tctcggcgtc tggctgaagc cggaaggttt
    45301 cccgcatcag gaaattcgtc agtactcttt gactcgcaaa ccggatggca aaggctatcg
    45361 tattgcggtg aaacgcgaag agggtgggca ggtatccaac tggctgcaca atcacgccaa
    45421 tgttggcgat gtcgtgaaac tggtcgctcc ggcaggtgat ttctttatgg ctgtcgcaga
    45481 tgacacacca gtgacgttaa tctctgccgg tgtcggtcaa acgccaatgc tggcaatgct
    45541 cgacacgctg gcaaaagcag gccacaccgc acaagtgaac tggttccatg cggcagaaaa
    45601 tggcgatgtt cacgcctttg ctgatgaagt taaggaactg ggtcaatcac tgccacgctt
    45661 taccgcacac acctggtatc gtcagccgaa tgaagccgat cgcgctaaag gtcagtttga
    45721 tagcgaaggt ctgatggatt tgagcaaact ggaaggtgcg ttcagcgatc cgacaatgca
    45781 gttctatctc tgcggcccgg ttggcttcat gcagtttgcc gcgaaacagt tagtggatct
    45841 gggcgtgaaa caggaaaaca ttcattacga atgctttggc ccgcataagg tgctgtgatt
    45901 tgatgttgcc ggatggacac atccggcaac ccttgacgcg ttttaaattg ccgcgtcgtt
    45961 ctcttcaccg gtacggatgc gaatgacccg tgccacgtcg aagacgaaga ttttaccgtc
    46021 accgattttg ccagtttgcg ccgtgcgaat aatggtatcg acacaggtat cgacaatgtc
    46081 gtccggtacg acaatctcaa ttttcacttt cggcagaaaa tccaccatat actccgcacc
    46141 gcggtacagc tcggtatggc ctttctggcg gccaaaacct ttcacctcag tgaccgtcat
    46201 gccggtaata ccgacttcag ccagtgcttc gcggacatcg tccagcttga agggtttaat
    46261 aatcgcatca atctttttca tgctattcct tgaaaaggtc acctgtcttt tgatctgcta
    46321 aacgtaacac ataacgccaa ttcattcctt gaaatcgttt gcatccagtt cgtgtcggga
    46381 aagcagttta taaaattctg tccggttgcg ccccgccatt ctcgccgcgt gggtgacgtt
    46441 gcctttggtg atttgcagca gcttacgcaa atagttgagt tcaaactgat tacgcgcctc
    46501 aacaaaggtt ggcagcgccg tgttttcgcc ctccagcgcc tgctccacca gcgcatcact
    46561 aattaccgga gatgatgtca gcgccacaca ctgttcaatc acgttgacca actggcgcac
    46621 attacccggc cagctcgcgg tcatcaggcg tttcatcgca tcggtggaga acgcgcggac
    46681 aaacggttta tgtcgctctg ccgcctggcg caacaggtga tttgccagta gcggaatgtc
    46741 ttctgtgcgc tccgccagtg ccggaatttt caggctgaca acattgaggc ggtaataaag
    46801 atcttcacgg aattccccac gcgccatcgc ttttggcagg tcgcggtgag tggcggaaat
    46861 aatccgcacg tcaatatcaa tatcgcggtt actgcccagc gggcgcactt tacgctcctg
    46921 caacacgcgc agcagtttga cctgcaacgg tgcgggcata tcgccaatct catcgagaaa
    46981 tagcgtaccg ccttccgccg cctggaataa accttcgcga ttgctgacag cgccagtaaa
    47041 cgcgccacgc gcatgaccaa atagctccga ctccagcaat tgctcgggta atgcgccaca
    47101 gttaatagca ataaatggct tgctgttgcg cgggctggcg ttgtggatag cctgggcgaa
    47161 aatctctttc ccggtgccgc tctggccgtt aatcaaaacg ctaacgtctg attgtgccac
    47221 cagccgcgcc tgttccagca agcgcagcat cagcgggctg cgggtgacaa tcgcctcgcg
    47281 ccaacgttcg tcggtggctg gcgcggattg ttccagcgca tcgtcaattg cctgatatag
    47341 cgcatcttta tcgacaggct tggtgagaaa actgaacacg ccctgctgtg ttgcagcaac
    47401 ggcatcagga atagaaccat gcgcggtaag aataattact ggcattcccg gctgcacttt
    47461 ctggatttca gcaaacagct gcataccgtc catttcatcc atccgcaggt cgctgatgac
    47521 taaatctact ttttcgcgat tcagtacccg caatccttca gcaccacttt ccgccgtgac
    47581 cacactgtag ccttcgctgg tcaggcgcag gccaagcagt ttcagcaatc ccggatcgtc
    47641 atcgaccagt aataaatgcg caggtttatg gctcatcagg agtgacctca tcggtggatg
    47701 gcgcgggctt ttcactctcg tgcggcgtat ccggcgagaa atttccagcc ggtttgcggg
    47761 tcgagagctg gcgttcaata tcggtcaggt tttccagctt gcgggtggtg agttccagtt
    47821 gctgctgtag aacgtgatat tgctggcgca aagtatccag ctcgctgtcg ctggactgct
    47881 ggagtttact gtagcgttgg cgctcttccg ccagttgcag ttgcagcgcc tgaccgtcgc
    47941 gccagagttg atacagaggg cgaacctgtg ccgggatctc ggtacttagc gcatcaatgc
    48001 gcgcgaccag ctggcggcgc tcatacggcg taattttggc gtcggcgagc aaaatccctt
    48061 gtttaaaggt attttgccag ttgccgtcgt catattggcg ggcttgctga cgcgactgcg
    48121 caggcattaa acgatcagca caatccatcg cccgcagcca gtaaagcgga ttggtttcgg
    48181 tagatttacc ttgcagcgcc cagatgtcgc tacattcagt agaaagatag tcagccagtt
    48241 gataaaccgg aattctttct tctgctggcg tatcaatggc tggcctattg tgactctgca
    48301 cgcaacccag caataccaga catggcagcc ctgccagcca cagccgtcgg ggcaataatc
    48361 gttgaaaaat gtgtcgcata ttcaccagac ttaaagccta tcccagtggg cgtaattgtt
    48421 gcaggcagtt tggacatgga cagcgcggag aaaccggagc gtacatgtcg tacgtgaaga
    48481 tttcgagcac tgcccggggc cgaactgaca aataaaatag cctgatggga tgggctgtta
    48541 gcattatttc gtgtttttcg acgacggtaa ttcaatgcgg aaacaaacgt cttgcccgct
    48601 ctcgtcgacc agatacagtt ccccttgcat acggcgaata caatccctgg caatgcttaa
    48661 tcccagaccg ctgcccttca ccgccccttt tcgctggtgg cttccctgaa aaaagggttc
    48721 gaagatcatg gcgcgttcct cttgcggaat gggcgtgcct gtattgatga catcaatata
    48781 aacccgcgca ccatgtaaac tgctgcgaag gcaaatgtta ccggattcag ccccgtagtg
    48841 caccgcattg gagtaaagat tatccagtac gctcatcagc agcattggct ccgccaggca
    48901 agctgttgct ttgagatcaa cgtcggtatg catcatttta gcccgtgcgg gcaggctatg
    48961 agcagaaacc actgtctcca ccagcggtgc taactcaaca ttctccagtt ccaccgcact
    49021 gtccgcctgt ttacggttgt aatcaagcag ttgttcgatc agcttttgca aattgcggct
    49081 gctgctatca agaatgctca ccacctcttt ttgctctggc gtaagcggcc cgacaacctg
    49141 atcagccagt aattcagtgc cctcgcgcat actcgccagt ggcgttttta attcatgaga
    49201 taaatgtctt aaaaattgat ggcgttggga ttccagccat gacaggcgct cacttaacca
    49261 aagaatacgt tgcccaaccg agcgtaactc gctcggtcca ctgaacgaga cgctattgcc
    49321 cagagaacgc ccttccccca gccggttgat catgcgctcg atatttttca ccggaccgat
    49381 aatcatcctc gtgaaaagca gtaccatcac cagactcacc agaaatagca ccagcgattg
    49441 ccaaccaaaa tattgcccac gttcggcgat ttcacgctga agttgctgcc cacgagagaa
    49501 caccactgtg cgcgtggcct gcaccatttc ggtattggca ctggcaaagg cttccagacg
    49561 cgcggcggca gcagcatcgg gaccgctgtt gttacactga agttgagcca gattattcaa
    49621 atcctgacgt aatgcctggt agagtttatc gtccggcagc acgcctgcgt gggcatcgag
    49681 catttcgctg taacgcttgc gctggctttg ataaaccttc gcaagcgtag ggtcgtccag
    49741 cacgcagtac tgacgatagc tgcgctccat ctccagtgcc gcgttggtca tcgcttcact
    49801 gcgccgggca tcaataagcg tagtacggtt aaccagcgcc gcctgatcgc taagtgcatt
    49861 caggctttgc caggcttgcc atgccagcac caacaggggc agcagaatca gcaaaaatgc
    49921 cagcattacc agttgtcgta atgagcgggg aaaaacgggc cagcgtttca aggcgttact
    49981 ctcgtcagac gcgaatagcc tgatgctaac cgaggggaag ttcagataca acaaagccgg
    50041 gaattacccg gctttgctat ggaataaggc ggtgcctaac tcgacgtttc gcccgatggt
    50101 tgatatagct acgctgatat cagaagttgg acggcaggca ccttgttgtg cgtcattcga
    50161 attttatgta gcacgtcccg aaggggctga cataagtcgg tgaatgagcc actgcttact
    50221 attatgcagt aactgtgcca ataaagaaaa tagtttggta acgtactgat tatacgctgc
    50281 tttgaggggt ttatggcttc tccgctattt gaatgtaaat cagccactgt gtcgctaaaa
    50341 agagacaact taagataaac ttattagata atattaaaat caatgagtta agtgtcacca
    50401 aaaagcgaca cggcaaacca cccattgtcg tgatttaaag acacaaaaaa gctcccgaag
    50461 ttgggagctc ataatagcgg ttggtgctta atgccggatg cggcgtgaat gcctcatccg
    50521 ggctgcaata ccaatgggtt gacgacttac cccaactgct tacgtgcatt gcggaaaatg
    50581 cgcatccatg ggccatcctc gccccagttt tccggatgcc aggagttgct gacagtacgg
    50641 aaaacacgtt ccgggtgcgg catcataatg gtgactcgac cactttcagt cgtgactgcc
    50701 gtaataccat tcggagaacc gttcgggtta gccgggtagg tttcagtgac tttgccgaag
    50761 ttatcgacat agcgcagtgc caccagccct ttgctttcca gtgccgccag atgcgccgca
    50821 tcacgcactt ccacgcgccc ttcaccgtga gagacagcaa tcggcatctg cgagcccacc
    50881 atcccctgca acagcagcga cgggctttgg gttacttcta ccagactgaa acgcgcttca
    50941 aagcgatcgg aggtgttacg cacaaaacgt ggccacaact cgctacctgg gatcagttca
    51001 cgcagattag acatcatctg gcaaccgtta cataccccca gcgccagcgt ttgcggacga
    51061 tggaagaagg ttgcaaactc atcgcgtaca cggtcattga acaggataga cttcgcccaa
    51121 ccttcaccag cgcccaacac atcaccgtag gagaaaccac cgcacgctac cagggcgtgg
    51181 aaaccctcca ggcccgtgcg tccagccagc agatcgctca tatgcacgtc gatagcatcg
    51241 aagcccgcac ggtggaaagc tgccgccatt tcaacatggg agttcacacc ctgctcacgc
    51301 agtacggcaa ctttcgggcg cgcaccagtg gcaataaacg gtgctgccac atcttcgttg
    51361 atatcaaacg acagtttcac attcaggccc ggatcggcgt cgtttgattt cgcctggtgt
    51421 tcctggtcag cacactccgg gttgtcacgc agacgctgca tctgccaggt agtttctgcc
    51481 caccagacac gcaacgtggt gcggctttcg ctaaatacag tctgcccgtt ggcggtaatc
    51541 acaaaacggt caccggaaac cgcctgccca acataatgga cgcaatcagc aagcccatgc
    51601 tgtgccagta ccgcttcaac ggctgcacgg tcagcggctc gaacctgaat caccgcaccc
    51661 agttcttcgc taaacaatgc agccaggcga tcgtcaccca gagtggcgat atccgcgtca
    51721 atgccacaat gaccagcaaa ggccatttcc gccagcgtta ccagcaggcc gccatcagag
    51781 cggtcgtgat acgccagcag cttacgctgt gcaaccagcg cctgaatcgc gtcatagaag
    51841 cctttcagtt gggcaacatc gcgtacatct gccggtttgt cgccaagctg acgataaacc
    51901 tgcgccagtg ccgttgcgcc cagcgcgtta ttgcctttgc ccaaatcaat cagcaacagt
    51961 gcgttatctt cggtagaaag ctgcggcgta atggtgtgac gtacatcttc cacgcgggca
    52021 aatgcggaaa tcaccagcga caacggcgac gtcatttcgc gctcttcgtt accttcctgc
    52081 cagcgggttt tcatcgacat agagtcttta cccaccggta tcgtcaggcc cagcgccgga
    52141 caaagttctt cgcccacagc tttaacggct tcatacaggc ccgcatcttc accagggtgg
    52201 cctgccgccg ccatccagtt ggcggaaagt ttaatgcgtt tgatatcgcc aatttgtgtt
    52261 gcggcgatgt tggttagcgc ttcaccgact gccagacggg cagaagcggc gaaatccagc
    52321 agcgcaactg gcgcacgttc gccaattgcc atcgcttcgc cgtagtagct gtcgaggctg
    52381 gcagtagtga ccgcacagtt agcgaccggc acctgccacg gccccaccat ctgatcgcgc
    52441 gccaccatgc cggttacgct gcggtcaccg atggtcacca ggaaagtttt ctccgccacg
    52501 gtcggcagat gcagcacgcg tttcaccgcg tcagcgatgg tgatcccttc acgggccagc
    52561 gcgtcgcctt tcgctttcag cgtttgtaca tcgcgagtca tcttcggcgt tttgccgagt
    52621 agcacgtcca gcggcagatc gatgggttga ttatcaaaat gacgatcgtg cagagaaaga
    52681 tgtaattctt cggtggcctc gccaatcacc gcgtagggtg cgcgctcacg cttacacagt
    52741 tcgtcaaaca gcggtaactg atcggcagca actgccagca cgtagcgttc ctgggattcg
    52801 ttacaccaga tttccagcgg gctcatgccc ggttcgtcgc tcaggatatc gcgcagttca
    52861 aatttaccgc cgcgcccgcc gtcgctcacc agttccggca tggcgttaga aagaccgcct
    52921 gcgccaacgt cgtggataaa caggattggg ttggcatcac caagctgcca gcaacggtcg
    52981 atcacttcct ggcagcgacg ctccatctcc gggttgtcgc gctgtacgga agcaaagtcg
    53041 aggtcggcat cagactgacc agatgccatg gaagacgccg cgccgccgcc aagaccgatg
    53101 ttcattgccg ggccgccgag aacgaccagc ttcgcaccaa cgttgatctc gcctttttgt
    53161 acgtgatcgg cgcgaatgtt gccgatccca cccgccagca tgatcggttt gtgataaccg
    53221 cgcagctctt cgccattgtg gctgttcact ttttcttcat aagtacggaa gtagccgttc
    53281 agtgccgggc gaccaaattc gttgttaaac gccgcgccgc ccagcgggcc ttcggtcatg
    53341 atgtccagcg cggtgacaat gcgctcaggc ttaccgaaat cttcttccca cggctgttca
    53401 aagcccggaa tacgcaagtt ggatacggag aaaccaacca gaccggcttt cggctttgcg
    53461 ccacgcccgg tggcaccttc atcgcggatt tcaccgccgg aaccggtcgc cgcgcccggc
    53521 cacggagaaa tcgccgtcgg gtggttgtgg gtttcaactt tcatcagaat atgcgccggt
    53581 tcctgatgga aatcgtagcg gcctgtttcg tggtcagcaa agtagcggcc cacttcagaa
    53641 ccttccatta cggcggcgtt atctttataa gcagagagaa cgtgatccgg cgtggtttcg
    53701 aaggtgtttt tgatcatctt aaacagcgat ttcggctgct gttcaccatc gataacccag
    53761 tcggcgttaa aaattttgtg gcggcagtgc tcggagttcg cctgcgcgaa catatacagt
    53821 tcgatgtcgt tcgggttacg accaagcttt gtgaaagcat cctgcaaata gtcaatttca
    53881 tcttccgcca gtgccaggcc aagacgcagg ttagcgtcga ttagcgcctg acggccctgc
    53941 cccagcaaat caacgctggt gaccggagtc ggttgatggt gtgcaaacag ctgctctgca
    54001 tcatctaaag caaaaaagac cgtttccatc atgcggtcgt gcagttcagc ggtaacctgc
    54061 tgccattgtt cattggtcag cgtaccggct tctatatagt aagcaacgcc gcgctcaagg
    54121 cggttaacct gttgcagccc gcagttatgg gcaatatcgg tcgctttcga agaccaggga
    54181 gagatggtgc caggacgcgg ggtcaccagc aggagtttgc cttgcggggc gtggctggcg
    54241 agtgccgggc catatttcag caggcgttca agttgtgcgt gctcatcatc gtttaacggc
    54301 gcattgaggt cagcaaaatg gacatactcg gcgtaaatat tgtgaaccgg gagcctggca
    54361 gcctgaaaac gtgccagcag tttgttgatt cggaatgccg acaatgcagg cgaaccacgc
    54421 agaatttcca tcataagtct ctcgtcttct aagctttcgg tgtacccaag ggggggaaac
    54481 gggcgtcatt ataaagaatc tgatgcgctg acgaaaccgt ttgcgtggaa ataaaatcac
    54541 catcgtgaat tagcaacgcg tgccgccaat ggctgtaata agttgccaac tggcgcaggt
    54601 ttacgcaaaa tgccgctcat ttatgagtaa acctttcact attattacgt tttttcaagc
    54661 tgggacgcgc acgacacaga gaattaacta attgaaaaaa ttaaagatta attatctgtt
    54721 catcggcatt ctggcactgc tgctcgcggt cgctctctgg ccatccattc cctggtttgg
    54781 taaagccgac aaccgtatcg ccgccattca agcgcgggga gagttgcgtg tgagcaccat
    54841 tcataccccc ctgacttata acgaaatcaa cgggaaacct tttggcctgg attacgaact
    54901 ggcgaaacag tttgccgatt acctcggcgt aaaactgaaa gtgaccgtgc ggcagaatat
    54961 cagccagctg tttgacgacc tcgataatgg taacgccgac ctgctggcgg caggcctggt
    55021 ctataacagt gagcgggtaa aaaattatca gcctggccct acctattatt ccgtgtcaca
    55081 acaactggtt tataaagtgg gtcagtatcg cccacgtacg ctgggcaacc tgacggcgga
    55141 gcaactcacc gttgcaccgg gtcatgtggt ggttaacgat ctccagaccc tgaaagacac
    55201 aaaattcccg gaattaagct ggaaggtaga cgacaaaaaa ggctctgcgg aattaatgga
    55261 agatgtcatc gaaggaaaac tcgattacac cattgctgat tctgtcgcca tcagcctgtt
    55321 tcagcgcgtt cacccggaac tcgccgtagc gctcgatatc accgatgaac aaccggtgac
    55381 ctggtttagc ccgttagatg gcgataatac cctttccgcc gccctgctcg acttcttcaa
    55441 cgaaatgaat gaagacggta cactggcacg cattgaagag aaatacctgg ggcatggcga
    55501 tgattttgat tacgtcgata cgcgcacatt tttacgcgcc gtcgatgcgg tactgccgca
    55561 gttaaagccc ctgtttgaga aatacgccga agaaattgac tggcgtttgc tggccgctat
    55621 tgcttatcag gaatcgcact gggatgctca ggccacttca ccgacgggtg tgcgcggcat
    55681 gatgatgtta accaaaaaca ccgcgcaaag cctcggcatt acggatcgta ccgatgccga
    55741 acagagcatc agcggcggcg tgcgttattt gcaggatatg atgagtaaag tgccggaaag
    55801 tgtgccggag aacgaacgga tctggtttgc cctcgccgcg tacaatatgg gctatgcgca
    55861 tatgctggat gcccgcgccc tgacgacaaa aaccaaaggt aatcctgaca gttgggctga
    55921 cgtaaaacag cgtctgcctt tacttagcca gaaaccctat tacagcaagc tgacttacgg
    55981 ctacgctcgt gggcatgaag cctacgctta tgtcgaaaat attcgtaagt atcagattag
    56041 cctggtgggt tatctgcagg agaaagagaa gcaggctaca gaagcggcga tgcaactggc
    56101 gcaggattat ccggcggtat cgcctacgga gctgggcaaa gagaaatttc cttttctctc
    56161 gtttctttcc cagtcgtcat caaactattt gacccactct ccctctctgc tgttttccag
    56221 aaaagggagt gaagagaaac aaaattaatc tgtcgaggat tgcgcttttt tctgcgcttt
    56281 aatttcctgg cggcgcatgc gaaagaagtc actgagcaac gccgcgcact catccgccag
    56341 tattccttcc gtaatttcca cccggtgatt catacccgga tgatgtagca catccattaa
    56401 agatcccgca gcgccagttt tcgcgtcacg cgcaccaaag accacgcgac caatgcgact
    56461 gtggatcatc gctccggcac acattacaca gggttcaagc gtgacataca acgtggcgtc
    56521 gatcagacga taattttgca tcaccaagcc accctgccgt agggccatga tttctgcatg
    56581 tgcggtggga tcatgtcgac caatcggacg gttccagcct tcgccaataa cacgattgtt
    56641 atgcactaat accgcgccga ccggtacttc ccgctcatcc caggcacgct tcgccagcgt
    56701 cattgcatga cgcatccagt attcgtggct aaattcgact tcagacaaaa agaaaactcc
    56761 ggttataaaa gcgcggcgca ttatacacgg acgctatgct ttactctatt ccagttgctg
    56821 gagttcaccg cgcggggtta ctcgccagcg atgctgacag aaataaagca gcgggttgtc
    56881 ctgattactg tcgctatagc cactgtacag ccgcagcggc gtgccgattt tgcgctccag
    56941 ttgcgcgacc ttttcatgtc ccagacaacg catcgtcaat acccaaccac catagccacg
    57001 ctgaatctga ctggcgataa gattaacccg cggcagccag ggcgtatcga aataaaccgc
    57061 ttcaaccagc ggctgcggag agccggtaat caaccagata tcagcatcgg aacttaacag
    57121 gtaggtggtt aatcgctcct gaaccagcgg aaaggcggta acattgtcac gaaaccagcg
    57181 tacgaaatcg gcctgcagcg cctgcaaacg cgcttcattg tgaccaaaag tgcatcccca
    57241 cagaagcaga ctcatcggcc agcgtgccgc acgacctttt atcaataacg caatggctat
    57301 gatcggcaac aacggcagaa caagtaacgc attcagcggt tggcgacgca gtaaataacg
    57361 caaaaaactg ccgaacatat cctgctgatg caatgttcca tctaagtcaa aaaacaccac
    57421 acgacgctcg tgagttgcca aaccatactc ctctggactg aaacatcctg attaattttc
    57481 gctactacat agcctaacag atagatcatc acttttccgg caacaatcga ccgcatcatg
    57541 gctaactgga atttttaatt catggcaatt agcggcaatg gaatataaaa ttcactcgcg
    57601 tttgtctcat attgcctgtt gtcgccatta tttcatcgcg aggaaagata tgaacggctt
    57661 acttcgtatc cgtcagcgtt accaggggct tgcccagagt gataaaaaac tggcggatta
    57721 tctgctgcta caacctgata cggcgcgcca tttaagctct cagcaactgg ccaacgaagc
    57781 cggagtcagt cagtccagcg tcgtgaagtt cgcgcaaaaa ctcggctata aaggttttcc
    57841 ggcactaaag ttggcgttga gtgaagcgct ggcaagccag ccggaatctc cctccgtgcc
    57901 cattcataac caaatccgcg gtgatgatcc gttacggctg gtcggcgaaa aactgattaa
    57961 agaaaatact gccgctatgt acgcaacgtt aaacgttaat agtgaagaga aactgcatga
    58021 atgcgtggca atgttgcgct ctgcgcggcg gataattctg accggtattg gcgcttcggg
    58081 tctggtggcg caaaactttg cctggaagct gatgaagatt ggcttcaatg ctgccgcagt
    58141 gcgcgatatg catgcgctac tcgctacagt acaggcgtcg tcccctgacg atctgttatt
    58201 agccatttcc tacaccggtg tacgacgcga gttaaacctg gcggcagatg agatgctgcg
    58261 agtgggcgga aaagtgctgg ctattaccgg ctttactccc aatgccctgc aacagcgtgc
    58321 ttctcattgc ctgtatacca ttgccgaaga acaagcgaca aacagtgctt caatctctgc
    58381 ttgtcacgcg cagggaatgt taacggattt gctgttcatt gcgctgattc agcaggatct
    58441 ggaactggca ccagaacgta ttcgtcatag cgaagcgctg gtgaaaaaac tggtctgagt
    58501 aaagaatgcg cgtataatgc ccgcccggtt tgtgttgttt tgagagtttc cttatggcgc
    58561 tgttaatcac taaaaaatgc atcaattgtg atatgtgtga acccgaatgc ccgaatgagg
    58621 cgatttcgat gggtgaacat atctacgaga ttaacagcga taagtgtacc gaatgcgtag
    58681 ggcactacga gacaccaacc tgccagaagg tgtgtccgat ccccaatact attgtgaaag
    58741 atccgcaaca tgtcgagacg gaagaacagt tgtgggataa atttgtgctg atgcaccacg
    58801 cggataaaat ttaatattct gctctggaag tagagtgtta attattttat agaaaaatct
    58861 taacgctatt atatttattt aaatgatgac attagcataa tcattcacta agttaattta
    58921 tatagtatct gcccagacac ttatttatag ttattaaagg cgcgtccgac tggttcaccg
    58981 gacgcacctt aagtacgttt ccttgtgtta taagaacaga aggatcagct gtaaaacagc
    59041 aatgatgatt ttgataaccc gtttaatcag gtatcggcaa tcagtcattc gtttttcctt
    59101 aaacaaggaa tgcagccgtt cagattagcc cttacatctc cccaaaactg aacgtgcgag
    59161 ttattggaat cgagtgctgc actccacacc agagctttga cgacaccact cgtttcaatg
    59221 ggggaattca gtggcatggt gtaaagcaca gcaaaattgc caataatgaa accgatttta
    59281 atgtacttat caaaaagcgg tcacgttaat cttttcatca atgtcgattt cttcgtaaag
    59341 gctcgcgttt tttatgtcag agattgcaag ttgcttgtac aggtcaacga aattgatcca
    59401 taatcatttt cgttggaatc gtgtgctgca caaaattaaa gttaaaaagt aaaacccc
//
LOCUS       DAZFJL010000010        59232 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFJL010000010 DAZFJL010000000
VERSION     DAZFJL010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.010"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..1624
                     /gene="yqiK"
                     /locus_tag="ACHJ8C_000621"
     CDS             <1..1624
                     /gene="yqiK"
                     /locus_tag="ACHJ8C_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO5550530.1"
                     /translation="TAIIAVCILFIIGIIFARLYRRASAEQAFVRTGLSGQKVVMSGG
                     AIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFFVRVKPSVEGIATA
                     AQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENFVQGVQNTVAEDLS
                     KNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRRERNEVEQDVEVAV
                     REKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEAERRREAEQTRILA
                     ERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQA
                     EARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKE
                     AAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVI
                     EKSVEPMKSIDGIKIIQVDGLNRGGATGDANTGNVGGGNLAEQALSAALSYRTQAPLI
                     DSLLNEIGVSGGSLAALTSSLSSTTPVEEKAE"
     gene            1864..1923
                     /locus_tag="ACHJ8C_000622"
     CDS             1864..1923
                     /locus_tag="ACHJ8C_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5550531.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(2043..3476)
                     /gene="hldE"
                     /locus_tag="ACHJ8C_000623"
     CDS             complement(2043..3476)
                     /gene="hldE"
                     /locus_tag="ACHJ8C_000623"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO5550532.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3524..6364)
                     /gene="glnE"
                     /locus_tag="ACHJ8C_000624"
     CDS             complement(3524..6364)
                     /gene="glnE"
                     /locus_tag="ACHJ8C_000624"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO5550533.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPKWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSDGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGVLTAHMANVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLMLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(6387..7688)
                     /gene="ygiF"
                     /locus_tag="ACHJ8C_000625"
     CDS             complement(6387..7688)
                     /gene="ygiF"
                     /locus_tag="ACHJ8C_000625"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO5550534.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVEVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDMRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7930..8550
                     /gene="ygiM"
                     /locus_tag="ACHJ8C_000626"
     CDS             7930..8550
                     /gene="ygiM"
                     /locus_tag="ACHJ8C_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO5550535.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8614..9852
                     /gene="cca"
                     /locus_tag="ACHJ8C_000627"
     CDS             8614..9852
                     /gene="cca"
                     /locus_tag="ACHJ8C_000627"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO5550536.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALM
                     REMTHAGELEHLTPERIWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSSQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLAKLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRAGFEAADYPQGRWLREAWEVAQSVPTKAVVDAGFKGVEIREEL
                     TRRRIAAVAGWKEQRCPKPE"
     gene            complement(10040..10861)
                     /gene="bacA"
                     /locus_tag="ACHJ8C_000628"
     CDS             complement(10040..10861)
                     /gene="bacA"
                     /locus_tag="ACHJ8C_000628"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO5550537.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10952..11320)
                     /gene="folB"
                     /locus_tag="ACHJ8C_000629"
     CDS             complement(10952..11320)
                     /gene="folB"
                     /locus_tag="ACHJ8C_000629"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO5550538.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSTWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11425..12042
                     /gene="plsY"
                     /locus_tag="ACHJ8C_000630"
     CDS             11425..12042
                     /gene="plsY"
                     /locus_tag="ACHJ8C_000630"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO5550539.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12055..12987)
                     /gene="ttdR"
                     /locus_tag="ACHJ8C_000631"
                     /gene_synonym="dan"
     CDS             complement(12055..12987)
                     /gene="ttdR"
                     /locus_tag="ACHJ8C_000631"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO5550540.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDAIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            13194..14105
                     /gene="ttdA"
                     /locus_tag="ACHJ8C_000632"
     CDS             13194..14105
                     /gene="ttdA"
                     /locus_tag="ACHJ8C_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO5550541.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLRVHADLTFENLSHTRSAL"
     gene            14102..14707
                     /gene="ttdB"
                     /locus_tag="ACHJ8C_000633"
     CDS             14102..14707
                     /gene="ttdB"
                     /locus_tag="ACHJ8C_000633"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO5550542.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14755..16218
                     /gene="ttdT"
                     /locus_tag="ACHJ8C_000634"
     CDS             14755..16218
                     /gene="ttdT"
                     /locus_tag="ACHJ8C_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO5550543.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPLPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASNATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTIVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(16261..17274)
                     /gene="tsaD"
                     /locus_tag="ACHJ8C_000635"
     CDS             complement(16261..17274)
                     /gene="tsaD"
                     /locus_tag="ACHJ8C_000635"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO5550544.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQEALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17512..17727
                     /gene="rpsU"
                     /locus_tag="ACHJ8C_000636"
     CDS             17512..17727
                     /gene="rpsU"
                     /locus_tag="ACHJ8C_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO5550545.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17838..19583
                     /gene="dnaG"
                     /locus_tag="ACHJ8C_000637"
     CDS             17838..19583
                     /gene="dnaG"
                     /locus_tag="ACHJ8C_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO5550546.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHLRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADRNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19778..21619
                     /gene="rpoD"
                     /locus_tag="ACHJ8C_000638"
     CDS             19778..21619
                     /gene="rpoD"
                     /locus_tag="ACHJ8C_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO5550547.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDTADDDNSIDPELAR
                     EKFAELRAQYIVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(21697..22203)
                     /gene="mug"
                     /locus_tag="ACHJ8C_000639"
     CDS             complement(21697..22203)
                     /gene="mug"
                     /locus_tag="ACHJ8C_000639"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO5550548.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            22328..22403
                     /locus_tag="ACHJ8C_000640"
     tRNA            22328..22403
                     /locus_tag="ACHJ8C_000640"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22361..22363,aa:Ile,seq:cat)
     gene            complement(22457..23221)
                     /gene="nfeF"
                     /locus_tag="ACHJ8C_000641"
                     /gene_synonym="yqjH"
     CDS             complement(22457..23221)
                     /gene="nfeF"
                     /locus_tag="ACHJ8C_000641"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO5550549.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPGTHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHEGGVASSWAMQAQSGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            23509..24132
                     /gene="nfeR"
                     /locus_tag="ACHJ8C_000642"
                     /gene_synonym="yqjI"
     CDS             23509..24132
                     /gene="nfeR"
                     /locus_tag="ACHJ8C_000642"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO5550550.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(24239..25759)
                     /gene="aer"
                     /locus_tag="ACHJ8C_000643"
     CDS             complement(24239..25759)
                     /gene="aer"
                     /locus_tag="ACHJ8C_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO5550551.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSASAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            26177..27556
                     /gene="ygjG"
                     /locus_tag="ACHJ8C_000644"
     CDS             26177..27556
                     /gene="ygjG"
                     /locus_tag="ACHJ8C_000644"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO5550552.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(27598..27930)
                     /gene="ygjH"
                     /locus_tag="ACHJ8C_000645"
     CDS             complement(27598..27930)
                     /gene="ygjH"
                     /locus_tag="ACHJ8C_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO5550553.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            28149..29132
                     /gene="ebgR"
                     /locus_tag="ACHJ8C_000646"
     CDS             28149..29132
                     /gene="ebgR"
                     /locus_tag="ACHJ8C_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO5550554.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTSALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            29316..32408
                     /gene="ebgA"
                     /locus_tag="ACHJ8C_000647"
     CDS             29316..32408
                     /gene="ebgA"
                     /locus_tag="ACHJ8C_000647"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO5550555.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLLL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGEHVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEHDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNHQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            32405..32854
                     /gene="ebgC"
                     /locus_tag="ACHJ8C_000648"
     CDS             32405..32854
                     /gene="ebgC"
                     /locus_tag="ACHJ8C_000648"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO5550556.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVIYDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            32917..34350
                     /gene="ygjI"
                     /locus_tag="ACHJ8C_000649"
     CDS             32917..34350
                     /gene="ygjI"
                     /locus_tag="ACHJ8C_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5550557.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            34484..35554
                     /gene="ygjJ"
                     /locus_tag="ACHJ8C_000650"
     CDS             34484..35554
                     /gene="ygjJ"
                     /locus_tag="ACHJ8C_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO5550558.1"
                     /translation="MKLITAPCRALLALPFCYAFSVTADEARPAEHDDTKTPAMTSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GIMASAMYEALKGFYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQLFLMQYFYW"
     gene            35571..37922
                     /gene="ygjK"
                     /locus_tag="ACHJ8C_000651"
     CDS             35571..37922
                     /gene="ygjK"
                     /locus_tag="ACHJ8C_000651"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFO5550559.1"
                     /translation="MKIKTILTPVTCALLISFSAHAADADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVLVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSTERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTDNGEMLFAVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            38148..40166
                     /gene="fadH"
                     /locus_tag="ACHJ8C_000652"
     CDS             38148..40166
                     /gene="fadH"
                     /locus_tag="ACHJ8C_000652"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO5550560.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPNGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDANQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQSFDETILASGIVPRIPPIDGIDHPKVLSYLDVLRDKAPVGKKVAIIGCGGIGF
                     DTAMFLSQPGESTSQNIAEFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMLPGVSYQKIDDDGLHVVINGEPQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(40211..40627)
                     /gene="higA"
                     /locus_tag="ACHJ8C_000653"
     CDS             complement(40211..40627)
                     /gene="higA"
                     /locus_tag="ACHJ8C_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO5550561.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPSGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLAARFGISPALFID"
     gene            complement(40624..40707)
                     /locus_tag="ACHJ8C_000654"
                     /pseudo
     CDS             complement(40624..40707)
                     /locus_tag="ACHJ8C_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            complement(40898..42034)
                     /gene="rlmG"
                     /locus_tag="ACHJ8C_000655"
     CDS             complement(40898..42034)
                     /gene="rlmG"
                     /locus_tag="ACHJ8C_000655"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO5550562.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCVLAEHKPYSIGDSYISELATRENLRLNGIDEPSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            42119..42622
                     /gene="ygjP"
                     /locus_tag="ACHJ8C_000656"
     CDS             42119..42622
                     /gene="ygjP"
                     /locus_tag="ACHJ8C_000656"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO5550563.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            42699..43391
                     /gene="ygjQ"
                     /locus_tag="ACHJ8C_000657"
     CDS             42699..43391
                     /gene="ygjQ"
                     /locus_tag="ACHJ8C_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO5550564.1"
                     /translation="MLRAFARLLLRICFSRRTLKIGCLLLLIAGATLFIADRVMVNAS
                     QQLTWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKAIFCDYAGFSTLDSVVRANKVFSENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGLYTQLREKLARVSAVIDAKILHRQPKYLGPLVMI
                     GPFSEHGCPAKE"
     gene            43470..44456
                     /gene="ygjR"
                     /locus_tag="ACHJ8C_000658"
     CDS             43470..44456
                     /gene="ygjR"
                     /locus_tag="ACHJ8C_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO5550565.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTAYLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGALVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVNHPGLAVSRITAKLLTEIRRQTGVIFPADNVKL"
     gene            44739..45704
                     /gene="alx"
                     /locus_tag="ACHJ8C_000659"
     CDS             44739..45704
                     /gene="alx"
                     /locus_tag="ACHJ8C_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO5550566.1"
                     /translation="MNTVGTPLLWGGFAVVVTIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTQGREVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVE"
     gene            46028..47272
                     /gene="sstT"
                     /locus_tag="ACHJ8C_000660"
     CDS             46028..47272
                     /gene="sstT"
                     /locus_tag="ACHJ8C_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO5550567.1"
                     /translation="MTTQHSPGLFRRLAHGSLVKQILVGLILGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(47276..47827)
                     /gene="ygjV"
                     /locus_tag="ACHJ8C_000661"
     CDS             complement(47276..47827)
                     /gene="ygjV"
                     /locus_tag="ACHJ8C_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO5550568.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQRRGIDPFKVEKTPSAVDERG"
     gene            complement(47910..49397)
                     /gene="uxaA"
                     /locus_tag="ACHJ8C_000662"
     CDS             complement(47910..49397)
                     /gene="uxaA"
                     /locus_tag="ACHJ8C_000662"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO5550569.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIAHLHQLYSVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            complement(49412..50824)
                     /gene="uxaC"
                     /locus_tag="ACHJ8C_000663"
     CDS             complement(49412..50824)
                     /gene="uxaC"
                     /locus_tag="ACHJ8C_000663"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO5550570.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            51303..52601
                     /gene="exuT"
                     /locus_tag="ACHJ8C_000664"
     CDS             51303..52601
                     /gene="exuT"
                     /locus_tag="ACHJ8C_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO5550571.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            52731..53507
                     /gene="exuR"
                     /locus_tag="ACHJ8C_000665"
     CDS             52731..53507
                     /gene="exuR"
                     /locus_tag="ACHJ8C_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO5550572.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            53747..53830
                     /locus_tag="ACHJ8C_000666"
     CDS             53747..53830
                     /locus_tag="ACHJ8C_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5550573.1"
                     /translation="MLMYQTRRTYQDSNNIAVVHHLKPAWR"
     gene            53852..54514
                     /gene="yqjA"
                     /locus_tag="ACHJ8C_000667"
     CDS             53852..54514
                     /gene="yqjA"
                     /locus_tag="ACHJ8C_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO5550574.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            54518..54901
                     /gene="mzrA"
                     /locus_tag="ACHJ8C_000668"
     CDS             54518..54901
                     /gene="mzrA"
                     /locus_tag="ACHJ8C_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO5550575.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVSTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            55048..55416
                     /gene="yqjC"
                     /locus_tag="ACHJ8C_000669"
     CDS             55048..55416
                     /gene="yqjC"
                     /locus_tag="ACHJ8C_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO5550576.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLKARDY"
     gene            55454..55759
                     /gene="yqjD"
                     /locus_tag="ACHJ8C_000670"
     CDS             55454..55759
                     /gene="yqjD"
                     /locus_tag="ACHJ8C_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO5550577.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRNKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            55762..56166
                     /gene="yqjE"
                     /locus_tag="ACHJ8C_000671"
     CDS             55762..56166
                     /gene="yqjE"
                     /locus_tag="ACHJ8C_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO5550578.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            56156..56455
                     /gene="yqjK"
                     /locus_tag="ACHJ8C_000672"
     CDS             56156..56455
                     /gene="yqjK"
                     /locus_tag="ACHJ8C_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO5550579.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSATRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            56642..57034
                     /gene="yqjF"
                     /locus_tag="ACHJ8C_000673"
     CDS             56642..57034
                     /gene="yqjF"
                     /locus_tag="ACHJ8C_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO5550580.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGMNSLMFMKN
                     LTISGGFLLLAITGPGAFSIDRLLNKKW"
     gene            57104..58090
                     /gene="yqjG"
                     /locus_tag="ACHJ8C_000674"
     CDS             57104..58090
                     /gene="yqjG"
                     /locus_tag="ACHJ8C_000674"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO5550581.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     KGGFAAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQPKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            58373..58741
                     /gene="yhaH"
                     /locus_tag="ACHJ8C_000675"
     CDS             58373..58741
                     /gene="yhaH"
                     /locus_tag="ACHJ8C_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO5550582.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(58775..>59232)
                     /gene="yhaJ"
                     /locus_tag="ACHJ8C_000676"
     CDS             complement(58775..>59232)
                     /gene="yhaJ"
                     /locus_tag="ACHJ8C_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO5550583.1"
                     /translation="IVIAPDMHFRSSSEINSRKLYTLMNVYVAAPDHPIHQEPEPLSE
                     VTRVKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLGVATMPYPMV
                     EKDIAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFSGK"
BASE COUNT        14091 a        15763 c        15326 g        14052 t
ORIGIN      
        1 taccgcgatt atcgccgtat gcattctgtt tattattgga attattttcg ccaggctcta
       61 tcgtcgcgct tcggcagagc aagcatttgt tcgtactggt ttaagtgggc aaaaagtggt
      121 gatgagcggt ggcgcaatcg tgatgccgat ctttcatgaa ataattccca tcaatatgaa
      181 taccctgaag ctggaagtca gccgttctac tattgatagc ctgattacga aagatcgtat
      241 gcgtgttgat gttgtagtgg ctttctttgt gcgggtaaaa ccttcagtag aagggattgc
      301 caccgccgcc cagacgctgg ggcaacgcac cctgtcgcct gaagacttac gtatgctggt
      361 ggaagataaa tttgtcgatg ccctccgtgc aacagcggca caaatgacca tgcatgagtt
      421 acaggatacc cgcgagaact tcgtgcaggg agtacaaaat accgtagctg aagatctgtc
      481 gaaaaatggc ctggaactgg aaagcgtttc acttaccaac tttaaccaga cctcgaaaga
      541 acatttcaat ccgaacaatg cctttgacgc cgaaggttta accaaactga ctcaggagac
      601 ggagcgccgt cgtcgcgaac gtaacgaagt tgaacaggat gtggaagtcg cagtgcgtga
      661 gaaaaaccgt gatgcacttt cgcgcaagtt ggagattgaa caacaagaag cgtttatgac
      721 gcttgagcag gagcagcagg ttaaaacccg taccgctgag cagaatgcga aaattgcggc
      781 ttttgaagct gaacgtcgtc gtgaagcaga gcagacgcga attctggctg aacgacagat
      841 tcaggaaacg gaaatcgacc gcgaacaggc cgtccgctca agaaaggttg aggccgaacg
      901 tgaagttcgc attaaagaga tcgaacagca gcaggtcacc gaaatcgcta accagacgaa
      961 atcgatcgct attgccgcca aatcggaaca acagtcccag gcagaagcgc gtgctaacct
     1021 tgcactcgca gaagcagtaa gcgcccaaca aaacgtagaa accactcgcc agactgccga
     1081 agccgatcgt gctaaacaag tggcgttgat cgccgcagcg caggatgcag aaaccaaagc
     1141 ggttgaactg accgtgcggg cgaaagcaga aaaagaagcc gcagaaatgc aggcggcggc
     1201 tatcgttgag ttagccgaag ctacacgtaa aaagggtctg gcggaagcag aagcacaacg
     1261 tgcgctgaac gatgctatca acgtactttc tgatgaacaa accagcctta aattcaaact
     1321 ggcattattg caggcgctgc ctgcggtaat tgagaaatcc gtggaaccaa tgaagtctat
     1381 cgacggcatc aaaattattc aggtcgatgg gctgaatcgt ggtggcgcta cgggtgatgc
     1441 aaacacgggt aatgtggggg gcggaaacct ggcagaacaa gcgttatcag ccgcactctc
     1501 ttaccgtaca caggcaccgc tgattgactc cttgctcaat gaaattggcg tttcaggcgg
     1561 ctcactggcg gcattgactt catccttaag ctcaacaact cccgtcgaag aaaaagcaga
     1621 gtaaaccctc ttctatgaat aatccctggc ccaaaaagcc ggggattttt tatatctgcg
     1681 ttccgctaaa aggtgcaaat gatcaagctg ttgcagcatt ttgcgtgact gctcggggaa
     1741 ggcaaaactg cctctgggaa agcgttgcgc ggggtctggc gctcatcaac aatcggggag
     1801 tcagcaaggg gctgaaacgg gaaagcccct cccgaggaag gggccttgta taaggaaagg
     1861 gttatgatga agctcgtcat catactgatt gtgttgttac tcgtaagttt cgcagcttat
     1921 taacagccaa tcagaggggg gagaaatcct ccctcaccct tgttccttta ctttaggttg
     1981 aaaaaaaatc agcgtcaata ggcccgcctg ctacaaagcg agatctgtga accgctttcc
     2041 ggttagcctt ttttatcctg ttggatcttc ttgatgatgt tggtcgtcga gcaaccgtct
     2101 tcaaagttga gcaccagcac ttcgccaccg ttggcccaga cttctttact cccggcaatc
     2161 tcttctggtt tatagtcgcc gcctttcacc agtagatctg gcaagatccc ggcgatcaag
     2221 cgctgcggcg tgtcctcttc aaacgacacc acccagtcga ccgcttccag tgcgcccagc
     2281 acaatcatac gctgttcgag cgggttaacg gggcgggaat cccctttcag ccgtttggtg
     2341 gaggcatcgc tgttcacagc gacaatcaaa cggtcaccca gtttacgggc atttgccaga
     2401 taagagacgt gcccggcgtg caggatgtca aagacaccgt tggtcatcac cactttttca
     2461 ccgcgtttac gcgctgccgc tacagcaagc ttcagttcct cttcggtcat cacaccaaag
     2521 ccggtatctg cacgtccacg tactgcgttt tccagctcga tcggcgaaac cgtggatgtc
     2581 cccagtttgc cgaccaccac accagctgcc gcattggcaa agaagcaggc ttcttccagc
     2641 gaattacccg ctgccagcgt tgccgccagg acgccaatca ccgtgtcgcc cgcaccggta
     2701 acgtcataca cttcctgcgc ctgggttggc atatgcagcg gcgctttacc cggttgcagc
     2761 agcgacatac cctgttcgga acgggtcact aacagagccg agagttcgta atcggcaatc
     2821 agtttcatgc cgcgctcaac aatctcttct tcggtcttac atttaccgac aacagcttca
     2881 aattccgaga gattcggcgt taacagcgta gcgccgcggt agcgctcaaa atcggtacct
     2941 tttggatcaa tcagcaccgg aacacccgct ttacgcgcca gttggatcat ctgctgtacg
     3001 cttgccagcg cacctttggc gtagtcagaa agcaccagcg cgccaatcga actcagtgcc
     3061 tgattaatcc gctcgtgcag cggctgcgga tcaacccctt cgaaaccttc ttcgaaatcc
     3121 agacggatca gttgttggtt gcgggaaagt acccgtaatt tggtgatggt cggatgcgtc
     3181 ggtacagaaa caaagtcgca tttgacgttg acgtcggcca gagatttact gagcgcgcgc
     3241 gccgcatcgt caatgcccgt caacccgacc aggcgtgcat tagcaccgag agaagcgata
     3301 ttcatcgcca cgttagccgc gccgcccgga cgttcttcga tggtattcac tttaaccacg
     3361 ggcaccggcg cttccggcga gatacggctg gtgggaccgt accagtaacg atccagcatc
     3421 acatcaccaa ccaccatcac tcctgcacgt tcaaactctg gcagcgttac tttcattcct
     3481 gtctcctgag agattcaaaa tttgcgcgcg ataataccat acttcattct tccaccagcc
     3541 acttctgcca gcttgcccgc accagttcac gctctgcggt gaagcaatcc tccgacacat
     3601 gacccggcaa ttcctgtaat gccagatgat gaagttcatc gcgcagcgtc gtgtaagcac
     3661 gggtcagcgc catcgcttcc tgctcttcca taatgtcgtt ttgcgccagt agttccagaa
     3721 tgcgcacgtt gtctgaccag cgcgttaact tcggtttttc gtgagcgtag cgcaacacca
     3781 gatattgagt aataaattcg atatcggtaa ttccgccttc atcggctttg atatcaaagc
     3841 gatcgcgatg tttattgccg agatgggcac gcattttctc gcgcatttcc cgcacttctg
     3901 tttgcagggt tttgccttcg cgcggcagcg tcataatctc ccggcgtact gcgtcaaagt
     3961 gcgcggtgag ctgcggatcg ccgtacacta cacgcgcacg caccagcgcc tgatgctccc
     4021 acgtccaggc ctcgtttttc tgataatcag caaatgcttc tgcggatgtc accagcattc
     4081 ccgccgcccc ggacggacgc agtcgtgcat ccacttcata caaaattccg gaagaggttc
     4141 gcgtactgaa cagatgcata atgcgttgcg ccagacgcag ataaaactgt cgcccgtcga
     4201 tttcccgctc gccgtcagtc atcgcatcca ttgggcaatc atggaggaag atcaggtcaa
     4261 gatcggaact gtagcctaac tcccagccac ccagcttgcc gtagccgacc accgcaaaac
     4321 cacgcccttc gcgttcgttc aggtgatttg gcttaccgta gcgggcaacc atttgaaccc
     4381 acgcctgctg aacgacggca tcaatcatgg cttctgccag ccaggttaag tgatcgctca
     4441 ctttcattac cggtagcgta ccggcgatat ccgctgccgc aatgcgtaac agctgcgcct
     4501 gtttgaactg acgcagcgcc tcaagctgtt gttcttcgtc atcttccggc acgcgcagca
     4561 aatactggcg cagttcatca cggtaggcat ccgtcgccgt tggttggtag agggtatttg
     4621 gatcgagcaa ttcatccagc aataatggat aacgcgctag ctggctggca atcatcggcg
     4681 atgcagcaca cagagaaatc aaatgtttaa gcgccgcagg gaattcacta agcagctcaa
     4741 gataagtggt gcgagtaaca atccccacca gcaaggcggt aatgcgcgac agcgtgacag
     4801 cagcgtcttc acgcgcacag acatcactta gcagatgtgg catcagatgg tcgagcacct
     4861 gacgtcctcg cggcccgata gtgcgcttat ccagctcttt gcggaaatca gcaatcagca
     4921 ttagcacctg tttgcgatca tcctcgctaa gatgcgccag cactggcgtg gtgtcatctt
     4981 cctgcaacgc atcctgccac agctcacgcc actgttccga cagcgactct tcctgagttt
     5041 cactttcgtc gtcgccaatc aattcattaa acacccggcg cacattggcc atatgtgcgg
     5101 tcagcacccc ggtcaattgc ggccagtcag caaagtccat cgcccacgcc agccgcgcac
     5161 ggttaagctc atcagaagga agcgtctggg tttgttcgtc gttaatgctt tgcagcaggt
     5221 tttccagacg ccgcaggaac agatacgcca ctcgcaattg ttcagcatcg ttttcagaaa
     5281 gcagatgcag cgcggcaatg gcgctgagcg ttggcagtaa agagcgcgat tgcagcgacg
     5341 gttcgcgccc gccgcgaatg agctggaaca cctggacgat aaattcaatt tcgcgaatgc
     5401 cgcctgcgcc aagtttgatg ttgtcggtca aaccgcgtcg acgcacttca cgggcaatca
     5461 ttcctttcat gttgcgcagc gactgaatca cgctgaaatc gatgtaacga cggaaaacaa
     5521 acggacgcag catcgcacgc aactcgttag catagacgcc gtcgctatcc cccataatcc
     5581 gcgccttgac catcgcgtaa cgctcccagt cgcgcccctg ctcctggtaa taatcttcca
     5641 gcgccgcaaa actcagcacc agcgggccac tttcaccaaa cggacgcagc cgcatatcca
     5701 cgcgatagac gaagccatcc tgcgttggtt gatccagcac tttaatcagc cgctgcccca
     5761 tgcgggtaaa aaactgcgcg ttatccagtt cccggcgtcc accctgggta caaccatgtt
     5821 ccggccaggc aaaaatcaga tcgatatcag aggagaaatt cagttccccg ccgcccagct
     5881 tacccatgcc taaaatcagc agcggttgcg cttcgccctg cgcattgcac ggcgttcccc
     5941 attcgcggca gcaggcgtca tacagccagt cacgcgccgc aacaatcagc gtctccgcca
     6001 gatggctgag ctgctgcaat atgctctctt cagtaaccag agccagcgtt tgcgcccagg
     6061 cgatgcgcac cataatgcgc cgccggaata gccgcagctc gcgcattaac ccggcttcgt
     6121 cactcacatt actgagcgcc tcctgcaacc atgcagcgta atgctgccat tcgtcggcct
     6181 gcggcggttg gctttccagt tccgtcagcc actttggatg cgcaatcacg ctgtcctgca
     6241 caaaatcact aaaagtaagt actgacttcg cctgtgcgct aagtgattcc tcggctaaag
     6301 gctctggcag ccgctcaaca acggtctgcc agtactgctg taacggtgaa gagagcggct
     6361 tcataagcga ttttatcctt gcctgattaa cgttttccgc tgtgcaacca gaacggttcc
     6421 tgattgtttg cctcattacg gaaatgttca atttcaatgc gttgcccggt cgcaatagcg
     6481 tgacgcagcc cctgccagtt ctccagccag gcttgcgcga cgacaggatc gtagtaaccc
     6541 gccagcaaca gtattgagtc aatatcacgc gtcaggcgtg gcaattggtc acggtagcga
     6601 tcgcctaacg gctggcagaa aacgctttta agttcagcag catggcggga aagatggata
     6661 tcggcaaagc gtttgaagga atcgctcatt ttactctgcg ctttggcatc taaaaacggc
     6721 tgccaggctt tgcttaccag ccattcggtc aacgccagct tcgccattgc cgtttcggta
     6781 gagtagaccg ccgttacggc agaaaccgca gaagcaatgg tcgcctcgca ttgagtcagc
     6841 agatcacgta agtgagtgct cgctttacgc ggcacgatac cgccgaacag catcagtgta
     6901 tgacggacca ggccaatggc tgccagcacc tgctctttcg ccgcatcgtt gccgcgtacc
     6961 cacagttctt catgatactg ccattgcgct aacgccagct cgagcgccgc ttccagcccc
     7021 tgttccacat cagcttttgc cgcaacatgc aaaatggtgg tcggtttgat ttcacgcgcc
     7081 ggattgccct gcgccagatg atagccacgc gctgctttgc tcaggctgcc ctggcgtaat
     7141 ccggtttgcg ataccagttg gttcgccagt ttcagcaccg cacgcatgtc gccgctaagc
     7201 agttccagtt ccagctcaca gataggttca gcaaattcac ccgctttcac ttccccctgg
     7261 tcgagggcga tttcaatttg gctaccatca acctctacca gccacttttc gcgataaaaa
     7321 tcggtgctga acagcggttg tacgcgggaa gcgagatcgg caggcaattc gccgttcggc
     7381 cagacttccg tcggtaactg cgccaggtcg agcgtcggtt cgctcaacgc cacgttatat
     7441 tccgggcgct ggtgtaagcc gccagtcact cttcctgcaa ctttcatggt catctcatag
     7501 cgaccgtttt cgccacgaat acgcaagccc atgtcgtgcc cacgcagcca gttatccggc
     7561 gtttcgtagt aaatattcag caactgcacg gggtcatggt gctcgccgcc cagcgtattg
     7621 agatggtcac gcagcgcctc aacggcactg tgattaacaa taaactttaa ttcgatttcc
     7681 tgagccatag cctttactta tcggttatgt cacataagtg acgatgaacg gcgaacttaa
     7741 tgcgatcttt tttgtcagta gatagtattt tgcgccaaat tgccatgcaa cgggcaattt
     7801 gacgggcgta aaagtttgaa gcagtggcaa gacctgacac agaggattcc gattgatgtc
     7861 gaatgctttg cgtagcgaca ctgatgccac tactatcgtt ccactttcca tgacaataac
     7921 gacagcctga tgccaaaatt acgcctgatc ggattaactt tactcgcact tagcgcgact
     7981 gccgtctcac acgctgaaga aacgcgctat gtttctgacg aactgaatac ctgggtccgt
     8041 agcggtccgg gagatcatta tcgcctcgtg ggcacggtta acgccggcga ggaagtgacc
     8101 ttattacaaa ctgacgccaa caccaattat gcccaggtga aagacagctc tggccgtact
     8161 gcctggatcc cgttgaaaca acttagcacg gagccaagcc tgcgttcccg tgtgccagat
     8221 ctggaaaatc aggtcaaaac gctgaccgat aaactcacca atatcgataa cacctggaat
     8281 cagcgcaccg cagaaatgca gcaaaaagtg gcgcagagtg acagcgtgat caacgggtta
     8341 aaagaagaaa atcagaaact gaaaaacgag ctgattgtcg cgcagaaaaa ggtcgatgcc
     8401 gccagcgtac agctggatga caaacagcgc accatcatca tgcagtggtt tatgtatggc
     8461 ggtggcgtgc tggggcttgg cttgctgctt ggtctggtac tgccgcacct gatcccaagc
     8521 cgcaaacgca aagatcgctg gatgaactaa atcgccttct tcgttgcact gtcttattat
     8581 taggaattgt gtatttgacg aagggatgat ggcgtgaaga tttatctggt cggtggtgct
     8641 gttcgggatg cattgttagg gctaccggtc aaagacagag attgggtggt ggtcggcagt
     8701 acgccacagg agatgctcga cgcgggctac cagcaggtag gccgcgattt tcctgtgttt
     8761 ctgcatccgc aaacgcatga agagtatgca ctggcacgta ccgaacggaa atccggttcc
     8821 ggttacaccg gttttacctg ctatgccgca ccggatgtca cgctggaaga tgatcttaag
     8881 cgtcgcgatc tgaccattaa tgcgctggcc caggacgata acggcgagat tatcgacccg
     8941 tacaacggtc tgggcgatct gcaaaatcgt ctgttgcgcc atgtttcccc cgcttttggc
     9001 gaagatccgt tacgcgtgtt gcgcgtagcg cgttttgctg cgcgttatgc ccacctcagt
     9061 ttccgtattg ccgatgaaac tctggcgctg atgcgcgaga tgacccatgc gggtgaactg
     9121 gaacacctga cgcctgaacg gatatggaaa gagacggaaa gcgccctcac cacccgcaat
     9181 ccccaggtgt tcttccaggt actgcgcgat tgcggcgcac tgcgcgtttt attcccggaa
     9241 attgacgcct tgttcggcgt tccggccccc gctaaatggc acccggaaat cgacaccggt
     9301 attcacactc taatgacgct ctccatggcg gcaatgctga gttcacaggt cgatgtccgt
     9361 ttcgcaacgt tatgccacga tctcggtaaa gggctgacgc caccagaact ttggccgcgt
     9421 catcacggtc acggcccggc gggtgtgaag ttagtggaac aactgtgtca gcgtctgcgc
     9481 gtgcctaatg aaatccgcga cttagccaaa ctggtggcgg agtttcacga tctcattcac
     9541 accttcccaa tgctgaaccc gaaaaccatt gttaaattgt ttgattcgat tgacgcctgg
     9601 cgtaaaccac agcgtgttga gcaactggca ctgaccagcg aggcagacgt gcgcggcaga
     9661 gctggttttg aagcggcgga ctacccgcaa ggacgctggt tgcgcgaagc ctgggaagtg
     9721 gcacaatcag tgccgacaaa agccgttgtt gacgcgggat ttaaaggcgt ggaaatccgc
     9781 gaggagctaa cccgacggcg gattgcagcg gtggctggct ggaaggaaca gcgttgccca
     9841 aaacctgagt gagttttgga tattttgccg gatgcgacgt aagcgtctta tccggctaca
     9901 tgtcaacgac agttgtaggc ctgataagac gcgcgagcgt cgcatcaggc gttgattgcc
     9961 ggatgcggcg tgaacgcctt atccggccta cgcaatctga taagacgcgt cagcatcata
    10021 tccgacactg ttcttcacat caaaagaaca caacgtatac cgcagccgcc acaataaagc
    10081 gataaatggc gaacgggata aacgaaatgc gcttaatcaa ttgcaggaag gttttaatcg
    10141 ctatcagcgc caccacaaaa gcggtgataa acccaacggc aaacatcggg atatcgccgg
    10201 tcgtcaggaa gccccagctt ttgtagagat cgagcgccgt tgcgcccatc atcatcggca
    10261 ccgccagcag gaacgagaac tcggaagcag cgtaacggct cacccccatc agcatcccac
    10321 ctgaaatggt cgccccggaa cgggagaaac ccggccacag cgccagacac tggaaacagc
    10381 caatcataaa cgcctgacga taggtcatat catcaagacc cggcgcacgc ggctctttcg
    10441 gcttcaggca ttcggcggca atcagcaaca gaccgccaac gaccagtgca tacatgacat
    10501 ttatcgggtt aaacaatgat ttaatcgtgt cgtggaacaa cagccccagt accaccgccg
    10561 gaatcatccc cagcaaaatg tggatcagcg ttaaacgacc tttgctttct ccttcgtgct
    10621 gcaacgggcg accaaagtgg atgccaatca ggccaaacag acgccgccag aacatcacta
    10681 ctaccgccag aattgatcct aactggatca cgacttcaaa ggttttcgcc gtgtcgccct
    10741 caaaccccaa caagtgaccg acaataatca tatggcccgt gctggatacc ggcagaaatt
    10801 ctgtcaatcc ttcgaccaca cccaatattg ccgctatcag cagcgagtgc atatcgctca
    10861 tcaataaacc cctaaattat aaaaatgtaa cgcttgtccg aactactgca gtatgaccag
    10921 gttataaccg tttggtttaa cagctgtaaa attaattatt ttctttcaga ttattgccac
    10981 gctcaatgat gacgccaaca ttcgccgccc gcgccactgc gcctggcttg ctgagtttga
    11041 tacgcaccca cgtcgagttg aagcgtgcta acagcagctc cgccacctct tccgccacac
    11101 gttccaccag cgcaaaacgc gccccctcga cgtggctgac taccgtttct gcaatgtcag
    11161 cgtaactgag gcaatccgcc acatcatcac ttttcgccgc tttacggtta tcccacgcca
    11221 tttcgatatc gaacactaac ttctgttcga tggtctgttc ccagtcgtaa acaccaatag
    11281 tggtgattac cgaaagttgc tctataaata caatatccat cacgtcctgc ctgctttttg
    11341 gctaaccgga taccacttcc ggcgaaatgt gcgtattatc cacagattca tcgttgaaca
    11401 cgaattttca aaacggaaca gcttatgagt gcaatcgcgc ctggaatgat cctcatcgcg
    11461 tacctctgcg gctccatttc cagtgccatt ctggtttgcc gcttgtgtgg gttgcccgat
    11521 ccgcgaacca gcggctccgg caatccaggc gcaaccaatg tgttacgtat cggtggcaag
    11581 ggagcagccg tagcagtact gattttcgac gttctgaaag gaatgttgcc cgtctggggc
    11641 gcgtatgaat taggtgtcag ccccttctgg ctaggcttaa ttgccatcgc cgcctgtctt
    11701 ggacacatct ggcccgtttt cttcggtttt aaaggaggaa aaggcgttgc taccgctttt
    11761 ggtgccatcg caccaattgg ctgggatctc actggagtaa tggcgggaac ctggttactg
    11821 accgtgctat tgagtggtta ctcgtcgctg ggagcgattg tcagtgcact gattgctccg
    11881 ttttatgtct ggtggtttaa gccacaattc accttcccgg tttcgatgct ctcttgcctg
    11941 atcctgctgc gtcatcatga caacatccag cgtctgtggc gtcgccagga gacaaaaatc
    12001 tggacgaaat tcaaaagaaa gcgcgaaaag gatcccgagt gatttctggt ggatctacat
    12061 gacctgatag ccttcgtcgg gcttgcccag ccgttgctgg caccatgccg ccagaaattc
    12121 cacacagaca cgtaatttca tgctgcgata gagcggctcc cggtaaacag cccagatatt
    12181 ggcgctttgt gcatactccg gcaatactcg caccagtttg ccactctcca gaaacggcaa
    12241 cacatcccac tcggaacgca gcataatccc tttgccctcc agcgcccatt gcagcacaat
    12301 ctcgccgcta ttggaggaaa gatgtccgct taccttcacc gattttttct cctgaccgtt
    12361 ccccaactcc catattccgt gggtcatatc gcgctctttg gtcaccaggc agtcatgacg
    12421 acttaattcc tgtaaggatt gtggctgcgg atatttttgc agatactcag gtgctgcgca
    12481 taatattctt ttattttttg tcaaaagatg cgcaatataa taatcaggaa ttgcgtcatt
    12541 aatacgaata tccagatcaa tattatcctg cactaaatca atttgccgat cgaacaattc
    12601 aaaatgcacc tgtaactcag gataattgcg catcagttcg gtaatggctg gagcaatatg
    12661 gctgcgccca aaaccaaaac tgcaaccaat gcggatcatc ccctccgggc gcgttttgat
    12721 ttgcgtgacg tcatcgacca gtcgctgata ttgggtgagg atttccagcg catgttcgta
    12781 acaacgttgg ccactttcag tcagcgccac gccgcgagcg gagcggttca gcagagttgt
    12841 tgccagcgta ttttcaagga tttggatccg cttggtgaca aatgcaggcg tttgcccaag
    12901 cgtcgccgct gcagcactaa aactcccgct atggacgatt tccaccagta cctgcaagtc
    12961 tttggctaaa ggccagctat ttagcattgt taaatatccg cagttttgat aaacgcagtg
    13021 tatttcgcaa aacattacca ttccctgctc tggcgcaata ttcgttctta ttttcgcgtt
    13081 gattcacgaa ctgttaatta catattcacg gtggcaaaaa atataaaacc acatttttag
    13141 tggtagtttg tggcggtgaa tttttccaga caaatacata aactggagtt gccatgatga
    13201 gcgaaagtaa taagcaacag gcagtgaata agttgacgga gattgtcgct aactttaccg
    13261 ccatgatttc tacccgaatg cctgatgacg tggtggataa actaaaacag ctaaaggatg
    13321 ccgaaacatc gtcgatggga aaaatcatct accacacgat gttcgataac atgcaaaaag
    13381 cgatcgacct gaatcgtcct gcctgtcagg acaccggcga aatcatgttt tttgttaagg
    13441 tcggttcccg tttcccactg cttggcgagc tgcaaagcat actcaaacaa gccgtggaag
    13501 aggcaaccgt caaagcgcca ctgcgtcaca atgcggtaga aatttttgac gaagtaaaca
    13561 ccggcaaaaa taccggcagc ggcgtaccgt gggtcacctg ggatatcgtc cccgacggtg
    13621 acgatgcgga aatcgaagtt tacatggcag gcggcggctg cacgctacca ggccgatcga
    13681 aagtgttaat gccgtcagaa ggctacgaag gcgtagtgaa attcgtcttc gaaaatatct
    13741 ccaccctcgc cgtaaacgcc tgtccgccgg tacttgtggg cgttggcatc gctacctcgg
    13801 tggaaaccgc cgcagtgctc tcgcgaaaag ccattttgcg cccgattggc agccgccatc
    13861 ccaatccaaa agcggcagag ctggagctac gcctggaaga aggactcaac cgtctgggga
    13921 ttggtccaca agggctgact ggcaacagtt cagtgatggg cgtgcatatc gaatctgccg
    13981 ctcgccatcc gtcaaccatc ggcgttgctg tttctaccgg ctgctgggcg catcgtcgcg
    14041 gcacactgcg agttcatgcc gatctcacct ttgaaaatct gtctcatacc cggagcgcgt
    14101 tatgaaaaag atcctgacaa ccccgatcaa agctgaagat ctgcaagata ttcgcgtcgg
    14161 cgatgtgatc tacctgaccg gtacgctggt gacctgccgt gacgtttgtc accgccgatt
    14221 gatcgaactg aaacgtccga tcccttacga tctcaacggc aaagcgattt tccacgctgg
    14281 ccccatcgtg cgcaaaaacg gcgacaaatg ggagatggtg tccgtcggcc cgacaaccag
    14341 tatgcgtatg gaaagttttg aacgtgaatt tatcgagcag accggcgtga aactggtggt
    14401 cggcaaaggc ggaatggggc cgctgaccga agaaggctgc cagaaattca aggcgctgca
    14461 tgtgattttc ccggctggct gtgcggtact ggcggcaact caagtggaag aaattgaaga
    14521 ggtacactgg acagaactcg ggatgccaga gtcgctgtgg gtctgccggg tcaaagagtt
    14581 tggcccgctg attgtctcta tagataccca cggcaacaac ctgatagccg aaaacaaaaa
    14641 gctgttcgcc gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg tccattacat
    14701 caaataaccc tcccggagag gctcgcccct ctcctttttc gcaggcataa cacgatgaaa
    14761 ccttccactg aatggtggcg ataccttgcg ccgctggcgg tcatcgccat tattgctcta
    14821 cttccgcttc ccgcagggct ggagagtcat acctggctct actttgctgt ttttactggc
    14881 gtgatcgttg gactgatcct cgaacccgtg ccgggtgccg tggtggcgat ggtgggtata
    14941 tccatcatcg ccattctttc gccgtggctg ctgtttagcc cggagcagct cgcccagcca
    15001 ggttttaagt tcaccgccaa atccctctcc tgggcggttt ctggtttttc taattcagtt
    15061 atctggctga ttttcgccgc ctttatgttt ggtacgggct atgaaaaaac cgggcttgga
    15121 cgtcgcatcg cgctgattct ggtgaaaaaa atggggcatc gcacgctgtt tctcggctat
    15181 gcagtgatgt tctccgagct gatactcgcg cctgtaacac cgtccaactc ggcgcgtggt
    15241 gcggggatta tctatcccat catccgtaac ctgccaccgc tctatcaatc acaaccaaac
    15301 gacagcagtt cgcgcagcat tggctcgtac atcatgtgga tggggattgt tgccgattgc
    15361 gtgaccagcg ccattttcct gacggcgatg gctccaaatt tgctgttaat tggactgatg
    15421 aaaagcgcat ctaacgccac gctgagctgg ggcgactggt tcctcgggat gttgccgctc
    15481 agcattttac tggttctgct ggttccctgg ctggcttacg tgctgtaccc gccggtactg
    15541 aagtctggcg atcaggtgcc gcgctgggca gagacggaac tgcaggcaat gggcccgctc
    15601 tgttcgcgtg aaaaacggat gctggggctg atggtaggcg cactggtgct gtggattttt
    15661 ggcggtgatt atatcgatgc cgcgatggtc ggttacagcg tggtggcgct gatgctgctt
    15721 ctgcgcatta tcagttggga cgacattgtc agtaataaag cggcgtggaa cgttttcttc
    15781 tggctggcct cgcttatcac cctcgctacc ggactcaaca acaccggttt tattagctgg
    15841 tttggcaaac tgttagcagg cagcttaagc ggttattcgc caacgatagt gatggtggcg
    15901 ttgattgtgg tcttttatct gctgcgctac ttttttgcca gcgccacagc gtatacctcc
    15961 gctctcgcac cgatgatgat tgccgccgcg ctggcgatgc cggaaatccc gctgccggtg
    16021 ttctgcctga tggttggtgc ggcaattggt ctggggagca ttcttacacc atacgccacc
    16081 ggacccagtc cgatttacta cggcagtggt tatctgccaa cggcggatta ctggcgactg
    16141 ggggcgattt ttgggctgat attcctcgta ttgctggtga ttaccggctt actgtggatg
    16201 cccgtggtgt tgctttaagt gaggacggtg ccggaagttg ctctccggca cctaatagtt
    16261 ttacgcagcc ggtaactccg ccagcggcca gcgcggacgc acactcacgc cgagatccgc
    16321 cgtcgcgcct gctttaaacc gcaccattcc ggcataggcg atcatcgcac cgttatcagt
    16381 gcaaaattcc ggacgcgcgt agaacacttc gccacgacgc tttttcatca tttccgccag
    16441 tttcgcccgc agtgtgcggt tagcactcac gccgcccgcc attaccagac gcttgaagcc
    16501 cgtctgatcc agtgctcgct tacacttaat catcaacgta tcgaccactg catcttcaaa
    16561 ggcgcgggcg atatcggcac gcgtctgatc gtctgtgccg ttgtcacgta tggtatttgc
    16621 cgcgaaggtt ttcagaccag aaaagctgaa atccagcccc ggacggtcgg tcatcggacg
    16681 cgggaaaaca aagcgcccgg cagtaccctg agccgccatt ttagacagta acggtccgcc
    16741 aggataatcc agccccagca gcttcgcggt tttatcaaac gcttcaccag cagcatcatc
    16801 gatagactcg ccgagcagct cgtactgacc aatgccagtc acgctgatta actgcgtatg
    16861 accgccggaa accagcagcg cgacaaacgg aaattccggt gggttatctt ccagcatcgg
    16921 cgctaacaga tgtccttcca tatgatgtac cgggattgcc ggaacgtccc aggcaaacgc
    16981 cagagaacgc cccacggtcg cgccaaccag cagtgcgccg actaatcctg ggcctgcggt
    17041 ataggccaca gcatcaatgt cttttgccgt taaaccagac tcctttagcg cctcctggat
    17101 caacggtacg gttttacgca cgtgatcgcg ggaggccagt tcaggcacga cgccgccgta
    17161 gtcagcgtgc aatttcacct gactatacaa ttggttggct aacaaacctt tttcatcgtc
    17221 gtaaatggcg atgccggttt catcgcagga agtttcaata cccagtacac gcatgacttg
    17281 ttttacctcg ctttattacc gcgcagtgta ggaccaatgc gggttgatgt aaaactttgt
    17341 tcgcccctgg agaaagcctc gtgtatactc ctcaccctta taaaagtccc tttcaaaaaa
    17401 ggccgcggtg ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca ttttgaaata
    17461 agctggcgtt gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca catgccggta
    17521 attaaagtac gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa gcgttcctgc
    17581 gaaaaagcag gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa accgactacc
    17641 gaacgtaagc gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact ggctcgcgaa
    17701 aacgcacgcc gcactcgtct gtactaattc cccgagagcg ttgctctccg atcagaccga
    17761 gttgtagttg taaggccgtg cttccgaaag gaatgcgcgg cttattttcg tttatgaatt
    17821 gctaaaaatc ggggcctatg gctggacgaa tcccacgcgt attcattaat gatctgctgg
    17881 cacgcactga catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag cagggcaaga
    17941 atttccacgc gtgttgtcca ttccacaacg agaaaacccc gtcattcacc gttaacggtg
    18001 agaaacagtt ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc gacttcctga
    18061 tgaactacga caagctcgag ttcgtcgaaa cggtcgaaga gctggcagca atgcacaatc
    18121 ttgaagtgcc atttgaagca ggcagcggcc ccagccagat cgaacgccat ctgcggcaaa
    18181 cgctttatca gttgatggac ggtctgaata cgttttacca acaatcttta caacaacctg
    18241 ttgccacgtc tgcgcgccag tatctggaaa aacgcggatt aagccacgag gttatcgccc
    18301 gctttgcgat tggttttgcg ccccccggct gggacaacgt cctgaagcgg tttggcggca
    18361 atccagaaaa tcgccagtca ttgattgatg cgggcatgtt ggtcactaac gatcaggggc
    18421 gcagttacga tcgtttccgc gagcgggtga tgttccccat tcgcgataaa cgcggtcggg
    18481 tgattggttt tggcgggcgc gtgctgggca acgatactcc caaatacctg aactcgccgg
    18541 aaacggacat tttccataaa ggccgccagc tttacggtct ttatgaagcg cagcaggata
    18601 acgctgaacc caatcgtctg cttgtggtcg aaggctatat ggacgtggtg gcgctggcgc
    18661 aatacggcat taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc gatcacatac
    18721 aactgttgtt ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac cgtgcaggcc
    18781 gcgatgccgc ctggcgagcg ctggaaacgg cgctgcctta catgacagac ggtcgtcagc
    18841 tacgctttat gtttttgcct gatggcgaag accctgacac gctggtacga aaagaaggta
    18901 aagaagcgtt tgaagcgcgg atggagcagg cgatgccact ctccgcattt ctgtttaaca
    18961 gtctgatgcc gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg agtacgctgg
    19021 cactgccact gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt cgtcaggaat
    19081 taggcaacaa attaggcata cttgatgaca gccagcttga acgattaatg ccaaaagcgg
    19141 cagagagcgg cgtttctcgc cctgttccgc agctaaaacg cacgaccatg cgtatactta
    19201 tagggttgct ggtgcaaaat ccagaattag cgacgttggt cccgccgctt gagaatctgg
    19261 atgaaaataa gctccctgga cttggcttat tcagagaact ggtcaacact tgtctctccc
    19321 agccaggtct gaccaccggg caacttttag agcactatcg tggtacaaat aatgctgcca
    19381 cccttgaaaa actgtcgatg tgggacgata tagcagatag gaatattgct gagcaaacct
    19441 tcaccgactc actcaaccat atgtttgatt cgctgcttga actgcgccag gaagagttaa
    19501 tcgctcgtga gcgcacgcat ggtttaagca acgaagaacg cctggagctc tggacattaa
    19561 accaggagct ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga tcgggaagcc
    19621 cccgacagcc gcactgagag gcagcggcaa atatataagt acgccctcgt aattatcgtt
    19681 ggcggtaaac aaccgttgga tttcagcttt aacggctgaa ggacatcggg tcaatcgccc
    19741 aacaccaacc tcatgaaata agtgtggata ccgtcttatg gagcaaaacc cgcagtcaca
    19801 gctgaaactt cttgtcaccc gtggtaagga gcaaggctat ctgacctatg ccgaggtcaa
    19861 tgaccatctg ccggaagata tcgtcgattc agatcagatc gaagacatca tccaaatgat
    19921 caacgacatg ggcattcagg tgatggaaga agcaccggat gccgatgatc tgatgctggc
    19981 tgaaaacacc gcggacgaag atgctgccga agccgccgcg caggtgcttt ccagcgtgga
    20041 atctgaaatc gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa tgggcaccgt
    20101 tgaactgttg acccgcgaag gcgaaattga catcgctaag cgtattgaag acgggatcaa
    20161 ccaggttcaa tgctccgttg ctgaatatcc ggaagcgatc acctatctgc tggaacagta
    20221 cgatcgtgtt gaagcagaag aagcgcgtct gtccgatctg atcaccggct ttgttgaccc
    20281 gaacgcagaa gaagatttgg cacctaccgc cactcacgtc ggttctgagc tttcccagga
    20341 agatctggac gatgacgaag atgaagacga agaagatggt gacgacgata ccgccgatga
    20401 tgacaacagc atcgacccgg aactggctcg cgaaaaattt gcggaactgc gcgctcagta
    20461 cattgtaacg cgtgacacca tcaaagcgaa aggccgcagt cacgctgccg ctcaggaaga
    20521 gatcctgaaa ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc agtttgacta
    20581 cctggtcaac agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac gtctgatcat
    20641 gaagctctgc gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc tgtttaccgg
    20701 caacgaaacc agcgatacct ggttcaacgc ggcaattgcg atgaacaagc cgtggtcgga
    20761 aaaactgcac gatgtctctg aagaagtgca tcgcgccctg cagaaactgc agcagattga
    20821 agaagaaacc ggcctgacca tcgagcaggt taaagatatc aaccgtcgta tgtccatcgg
    20881 tgaagcgaaa gcccgccgtg cgaagaaaga gatggttgaa gcgaacttgc gtctggttat
    20941 ttctatcgct aagaaataca ccaaccgtgg cttgcagttc cttgacctga ttcaggaagg
    21001 taacatcggt ctgatgaaag cggttgataa attcgaatac cgccgtggtt acaagttctc
    21061 cacctacgca acctggtgga tccgtcaggc gatcacccgt tctatcgcgg atcaggcgcg
    21121 caccatccgt attccggtgc atatgattga gactatcaac aagctcaacc gtatttctcg
    21181 ccagatgctg caagagatgg gccgtgaacc aacgccggaa gaactggctg aacgcatgct
    21241 gatgccggaa gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa tctccatgga
    21301 aacgccgatc ggtgatgatg aagattcgca tctgggggat ttcatcgagg ataccaccct
    21361 cgagctgccg ctggattctg cgaccaccga aagcctgcgt gcggcaacgc acgacgtgct
    21421 ggctggcctg accgcgcgtg aagcgaaagt tctgcgtatg cgtttcggta tcgatatgaa
    21481 caccgaccac acgctggaag aagtgggtaa acagttcgac gttacccgcg aacgtatccg
    21541 tcagatcgaa gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg aagtgctgcg
    21601 tagcttcctg gacgattaat cggtaggccg gatcaggtgt tacgccgcac ccggcactag
    21661 tccctccgca caaacgccac cttttcggtg gcgtttttat cgcccacgca ccaccagcgc
    21721 ctggtccagc tcgcgatacg cttctaccag tttctccagt gaaacgcgac ttaaaccgct
    21781 gggatttggc aggacccaaa tctgcgtcga accaatggtg agagtttgtt tcccccactg
    21841 tgcaccgcgc tggctgaatc cctgttcata tgcttgtttg cccagaatcg ccaacgcctg
    21901 cggctgataa tcttctattt tttcaattag cttacgcccg cctgcgtgca gctcctgctt
    21961 tgacacttca ttggcttgca ccgttggacg gtctaccagt ttggtgacgc cacaacgata
    22021 atccagcaga tgctgtgcct cctgcggctt caactgacgg tcggtaaacc cggcctgata
    22081 tatcaccttc cagaagcggt ttgccggatg agcaaaggga aaacccgttc cggcggatga
    22141 aagaccaggg ttgataccgc aaaacacgac ccgtaaccct ggagccaaaa tatcctcaac
    22201 catatttact cctttctaac atcaccagga aaagtataac ggattgaaaa tacattgttt
    22261 ataaaaacag caggcgcgct gtaatggctg gattgcgaca cggagttact ttataatccg
    22321 ctatcatggc cccttagctc agtggttaga gcaggcgact cataatcgct tggtcgctgg
    22381 ttcaagtcca gcaggggcca ccagacatag caaaggctga cgagaaatcg tcagcctttt
    22441 tctttttata tatcagttac tttgcgtgcc agtaagccgc cgcgcgtacc cgctgtgggt
    22501 catactgttc cgcttcaaag cgacggctta aattcttaac gactttgcct tcgccggtta
    22561 tccagatgaa gtaatcatcg gcagggattt gcatctgcgc cagacgcgca tctaccgcct
    22621 gctcatcatg tgccagccat tcgatattaa aaccatcaag gtgcgcgaga taatcctgac
    22681 aggcgttatc ccgcacgcta accagcgcgc taacttgcgg tttaacggca agtttgctca
    22741 acgtttccag gcggcggcgc agtgcgggca ttccggattc atcgcagaca tacagctgat
    22801 acgcgtaatc ctctggcacc accagcgaac cgcgcggacc tgccaccgtg agtttatcgc
    22861 ccgattgcgc ctgcattgcc cagctgctgg cgacgccacc ttcgtgaata aagaaatcaa
    22921 tcgccagttc atggcgtagt tcgtcataca gcggcgtata gtcacgcgac ggtgggcgtg
    22981 gtccttccgg ccagacgatg ccctcttccg ttaccgttgg cggcacaaag tgagtgcctg
    23041 gttgaggaaa gaagagcttg ctgtgatcgt caaagccgcg cgatgtaaaa ccgtccagcg
    23101 cctcgccgcc aaggacaatg cgctgaaaac cggcgctgat acgctcaacg cgtagcacag
    23161 tcagttcacg gaagcgcaga tcattgcgaa cgcgctgcgg gtagcggggg gtgttattca
    23221 ttgttatcgc cttcgtgatg gtaatcagat atatctaaat aaattccaca aatgataatg
    23281 attgttaatc atgataaatg caagtgattt gtagcactga tatatctgta gtctgataag
    23341 acgcgcaacc tcttatcaag catcattgct caatgccgga tgcggcgtga acgccttatc
    23401 cggcctacat gttgagtaaa cctgcgttta aaaatcattt ttgtacttgc agtaatgctt
    23461 atatcagata taaattagat atatctaatt aagcaaaagg aggctgatat gagccatcat
    23521 cacgaagggt gctgtaaaca tgaaggccag ccacgccatg agggctgctg caaaggtgag
    23581 aagtcagaac acgagcactg cggacacggt caccagcatg aacacggtca atgctgcggt
    23641 ggtcgccacg gtcgcggcgg cggtcgtcga caacgtttct ttggtcacgg tgaattgcgt
    23701 ctggtgattc tggatattct ctcgcgcgat gacagccacg gttacgagtt gattaaagcg
    23761 attgaaaatc tgacccaggg gaattacacc ccaagcccgg gcgtcatcta cccaacgctg
    23821 gattttctgc aggagcagtc gctgattacc attcgcgaag aggaaggtgg taagaagcag
    23881 attgcgctga ccgaacaagg cgcgcagtgg ctggaagaaa accgcgaaca ggtagagatg
    23941 attgaagaac gcatcaaagc gcgttgcgtt ggcgctgcgc tgcgccagaa cccgcaaatg
    24001 aagcgggcgc tggataattt taaagcggtg ctggatttac gcgtcaacca gagcgatatc
    24061 actgatgcac aaataaaaaa gatcattgcg gtgatcgacc gcgccgcttt tgatattacc
    24121 caactggatt aatcaccgat tgccggatgc ggcgtaccct acacaccccg taggcctgat
    24181 aagatgcgcc agcatcgcat caggcattgt gctccaaccg ccggatccgg cataccgatt
    24241 aatgcagcac cgtcaccgcg tcttccagtc ggctggcgcg gtgtttcacc atcgccgaca
    24301 cctgtgcact ctcttccacc agctcggcat ttttctgggt gatcaggtta agctcatcca
    24361 ccgcacgggt caggctggaa agcccatctg cctgttccag cgttgaatgg ctaatctggg
    24421 cgatcaactg ggtgacgttt ttcacctgtg ccacaatatc ttccatcgtc cgtccggctg
    24481 catgtacctg ctgcgaaccg gattgcacct tatcagcact ggcatcgatc agcttgcgaa
    24541 tatcgttggc ggcactggcg ctgcggctgg ctaaatgacg cacttcccct gccaccactg
    24601 caaaaccttt gccttgttcg ccggcacgcg ccgcttccac cgccgcattc agggccagaa
    24661 tattggtctg aaacgcaata tcgttaatca gcgaagtaat ggtgccaatg cgctgtgtac
    24721 tgtcggcgat atcgtccatt gtcttgatca ccgtggtcat cgcctccccg ccctgcactg
    24781 ccgcattact ggcggtgatt gaaagtttat cggcagccga cgccgtggca gagttctgtt
    24841 tcaccgacgc cgccatttgg ttcatggtcg ccaccgtttg ctgaacgtta tcaactgtct
    24901 gctgggtatg ttcgttcagt tcatcggtgc ctttcgccag cgtctcactg ccatttctga
    24961 cactggacac ctggcttgag acatcgttaa ttagccaacg gcacatcagg ccaagctgcc
    25021 ctaccgcgcg taatgtcagc cccagctcat cgctgcgatt cagatgctca acactattac
    25081 gctctccggt cgccaccttc agcgcctgac gggcgacatt ttctatcggg cggacaatct
    25141 gccattcaaa acaagcgctt gccaacaata ccactaacgc acagaggata tacgtcacca
    25201 ccggggcagc aacaaaccaa agcatagccg ccagcaagat aaacatcagg gtcatcacgc
    25261 cacgcgcccg ccagcgaagc ggtaatgaag gcagtttacc cagccagcct ttacgcacca
    25321 ccaggccttt atgaatacgc ttactggtac gtccggcgtt caacgctttg tacagcggct
    25381 ccaccgccgc gatctcttca tccgtcgccc gggtacgaat cgacatatag ccactgattt
    25441 ttccctcgcg caccatcggt accgcattgg cccgcaccca ataatggtcg ccatttttgc
    25501 ggcgattttt cacgatgccg ctccagggct cccctttttt cagggtgaac cacatatccg
    25561 caaacgccgc ttttggcata tccgggtgac gcaccatgtt gtgcggctgc ccctgtaact
    25621 cttgcaaggt atagccgctc acctgcacaa aagtgtcatt agcatgagtg atatagcttt
    25681 gcagatcggt agtggacatc agagtggtgt cgtccgccag cggggtattt tgctgggtga
    25741 catacggatg agaagacatg ttcgcgtcct gtgcaggtta tatggttgtt aacttcttgt
    25801 cagagtttat gtcggccccg ctgcggttat ctttaaccaa ttaatttgat ttagatcgca
    25861 atttgcgatt aaagcacaaa tctaattcct tgatttaaaa tactttcact ctgttactat
    25921 acgaaaacgt taattatctt gcccaaaaat caggcaatta ttgccctgaa agcgtgcatt
    25981 tgcgcagcaa tcatcaaatc catacccgac aaaaaccgtg caaaataaca gcaaatgtta
    26041 gcaaatagca ttaaatattg cgcaaatgat aaccacattt gtgtttatcc cgattttcgc
    26101 gatcgcagcc ggagtggcgc aatccctgca atacttaaat cggtatcatg tgatacgcga
    26161 gcctccggag catattttga acaggttacc ttcgagcgca tcagctttag cgtgcagcgc
    26221 ccacgccctg aatctcattg aaaagcgaac gttggatcat gaggagatga aagcacttaa
    26281 ccgggaggtg attgaatact tcaaagagca tgtcaatccg gggtttttag agtatcgcaa
    26341 atctgttacc gccggcgggg attacggagc cgtagagtgg caagcgggag gtttaaatac
    26401 gcttgtcgac acccagggac aggagtttat cgactgcctg ggaggttttg gaattttcaa
    26461 cgtggggcac cgtaatccag ttgtggtttc cgccgtacag aatcaacttg cgaaacaacc
    26521 gcttcacagc caggagctgc tcgatccgtt acgggcgatg ctggcgaaaa cccttgcggc
    26581 gctaacgccc ggtaaactga aatacagctt cttctgtaat agcggcaccg aatccgttga
    26641 agcggcgctg aagctggcga aggcttatca gtcaccgcgc ggcaagttta cttttattgc
    26701 caccagcggc gcgttccacg gtaaatcact tggcgcgctg tcggccacgg cgaaatcgac
    26761 cttccgcaaa ccgtttatgc cactgctgcc agggttccgt catgtaccgt ttggcaatat
    26821 cgaagccatg cgcacggctc ttaacgagtg caaaaaaacc ggtgatgatg ttgctgcggt
    26881 gatcctcgaa ccgattcagg gcgaaggtgg cgtaattctg ccgccgccag gctatctcac
    26941 cgccgtgcgt aagctgtgcg atgagttcgg cgcactgatg atcctcgatg aagtacaaac
    27001 gggcatgggg cgcacgggca agatgttcgc ctgcgagcat gagaacgtac agccggatat
    27061 cctctgcctt gccaaagcgc tcggcggtgg cgtgatgccg attggcgcaa ctatcgccac
    27121 tgaagaggtg ttctcagtcc tgttcgacaa tccgttcctg cataccacca cctttggcgg
    27181 caacccgctg gcctgtgcgg cggcgctggc gaccatcaat gtgttgctgg agcagaactt
    27241 accggctcag gctgaacaaa aaggcgatat gttgctggac ggtttccgtc aactggcgcg
    27301 ggaatatccc gatctggtac aggaagcgcg tggcaaaggg atgttgatgg cgattgagtt
    27361 tgttgataac gaaatcggct ataactttgc cagcgagatg ttccgccagc gcgtactggt
    27421 ggccggaaca ctcaacaacg ctaaaacgat ccgcattgaa ccgccactga cactgaccat
    27481 tgaacagtgt gaactggtga tcaaagcggc gcgtaaggcg ctggcagcca tgcgagtcag
    27541 tgtcgaagaa gcgtaatatc aaatcggatg gcgatacgac gtcgccatcc gatcttttta
    27601 tacgatacgt acgcccgcag gcatcatccg ctccggcgtt aacagcacgc tttcactgcc
    27661 gtcatcggtt tccgcacaca acagcatgca ttccgacgtt tcaccgcgca ttttcgcttt
    27721 ttgcagattg cataagacca ccactgtttt tcccatcagc tcttcttcgc tgtagtacgg
    27781 caccagactg gtcacggttt gcagcgtttt ttcccccaca tcaacctgta cgatgtacag
    27841 cttgtcggcg ttttcatggc gtttcacttc cacaatcttt ccgacgcgca tttccagacg
    27901 tgcaaaatca gcgtaagcca cggtttccat tgtttcctcc cttcaagtaa aattttacta
    27961 aaatatagca aaagtttttc tcaatcctgt aggctaaaaa tggagaatgc aggcgtgatc
    28021 acattcgcaa gccgctgtgt taccgttaca gcgtcaaaga aacgcgcttt atttactgaa
    28081 aacaggtgac ccgataagca cttcctctac aatgggggcg cacatcaggg aaagtaaaaa
    28141 aggtaaacat ggcaacacta aaagacatcg caatcgaagc tggcgtatcc ctggcgacag
    28201 tatccagggt cttgaatgac gatccgacat tgaatgtgaa agaagagacg aaacatcgca
    28261 ttctcgagat cgccgaaaag ctggagtaca agaccagtag cgcccgtaaa ctccagacag
    28321 gcgcagtcaa ccaacaccat attctggcta tctacagcta ccaacaggag ctggagatca
    28381 acgatcctta ctatctggcg atccgccacg gcattgaaac tcagtgcgaa aagctggcga
    28441 tcgaactgac caactgttat gaacacaacg gcttaccaga cattaaaaac gtcaccggta
    28501 ttttaattgt cggcaaaccc acgtccgccc tgcgcgccgc cgcctgtgcg ttgaccgaca
    28561 atatctgttt tatcgacttt cacgaacccg gcagcggtta cgatgcggtg gatatcgatc
    28621 tggcacgtat cagtaaagaa atcatcgatt tctatatcaa tcagggcgtt aatcgtattg
    28681 gttttattgg cggtgaagac ttacccggca aggcggatat tcgtgaggtc gcctttgcgg
    28741 aatatggtcg tctgaaacac gtggtacgcg aagaggatat ctggcgcggc ggtttttcca
    28801 gttcgtcggg ctatgaactg gcaaaacaga tgctggcgcg ggaagactat ccgaaggcac
    28861 tgtttgttgc ttccgattcc attgctatcg gcgtactgcg ggcaattcat gaacgtggcc
    28921 tgaacatccc acaggatatt tcgcttatca gcgttaacga tatccccacc gcgcgattta
    28981 cctttccgcc gctctccacc gtgcgcatcc attccgaaat gatgggaagt cagggcgtta
    29041 acctggtgta tgaaaaagcc cgcgatggtc gcgcgctgcc gctgttggtc ttcgttccca
    29101 gcaaattaaa actgcgcggc acgacccgtt aaatcccctt gcacgctgtc cggcaatcgt
    29161 ttttgccgga cagtgctgcc gtttattttc gtgatccagt taaagtaaat gcatttatcc
    29221 gctacttttt agtaaaattt ttactaaact ccccagcaat tacacaaact accatcacca
    29281 tgaatggttc cgatttctct ctaccgggag gccctatgaa tcgctgggaa aacattcagc
    29341 tcacccacga aaaccgactt gcgccgcgtg cgtacttttt ttcatatgat tctgttgcgc
    29401 aggctcgtac ttttgcccgt gaaaccagca gcctgtttct gctcttaagc ggtcagtgga
    29461 atttccactt ttttgaccat ccgctgcaag tgccagaagc tttcacctct gagttaatgg
    29521 ctgactgggg gcatattacc gtccccgcca tgtggcaaat ggaaggtcac ggcaaactgc
    29581 aatataccga cgaaggtttt ccgttcccca tcgatgtgcc gtttgtcccc agcgataacc
    29641 caaccggtgc ctatcaacgt attttcaccc tcagcgacgg ctggcagggt aaacagacgc
    29701 tgattaaatt tgacggcgtc gaaacctatt ttgaagtcta cgttaacggt cagtatgtgg
    29761 ggttcagcaa gggcagtcgc ctgaccgcag agtttgacat cagcgcgatg gttaaaaccg
    29821 gcgacaacct gttgtgtgtg cgtgtgatgc aatgggcgga ctctacctac gtggaagacc
    29881 aggatatgtg gtggtcggcg gggattttcc gcgatgttta tctgatcgga aaacaactca
    29941 cgcatattaa cgatttcacc gttcgtaccg actttgacga agcctattgc gatgccacac
    30001 tttcctgcga agtggtgctg gaaaatctcg ccgcctcccc tgtcgtcacg acgctggaat
    30061 ataccctgtt tgatggcgaa cacgtggtgc acagcagcgc cattgatcat ttggcaattg
    30121 aaaaactgac cagcgccagt tttgctttta ctgtcgaaca gccgcagcaa tggtcagcag
    30181 aatcccctta tctttaccat ctggtcatga cgctgaaaga cgccgacggc aacgttctgg
    30241 aagtggtgcc acaacgcgtt ggcttccgtg atatcaaagt gcgcgacggt ctgttctgga
    30301 tcaataaccg ttatgtgatg ctgcacggcg tcaaccgcca cgacaacgat catcgcaaag
    30361 gccgcgccgt tgggatggat cgcgtcgaga aagatctcca gttgatgaag cagcacaaca
    30421 tcaactccgt gcgtaccgct cactacccga acgatccacg tttttacgaa ctgtgcgata
    30481 tctacggcct gtttgtgatg gcggaaaccg acgtcgaatc gcacggcttt gctaatgtcg
    30541 gcgatatcag ccgtattacc gacgatccgc agtgggaaaa agtctacgtc gagcgcattg
    30601 ttcgccatat tcacgcgcag aaaaaccatc cgtcgatcat catctggtcg ctgggcaatg
    30661 aatccggcta tggctgtaac atccgcgcga tgtaccatgc ggctaaggcg ctggatgaca
    30721 cgcgactggt gcattacgaa gaagatcgcg atgctgaagt ggtcgatatt atttccacca
    30781 tgtacacccg cgtgccgctg atgaatgagt ttggtgaata cccgcatccg aagccgcgca
    30841 tcatctgtga atatgctcat gcgatgggga acggaccggg cgggctgacg gagtaccaga
    30901 acgtcttcta taagcacgat tgcattcagg gacattatgt ctgggaatgg tgcgaccacg
    30961 ggatccaggc gcaggatgac aacggcaatg tctggtataa attcggcggc gactacggcg
    31021 actatcccaa caactataac ttctgtcttg atggtttgat ctattccgat cagacgccgg
    31081 gaccgggcct gaaagagtac aaacaggtta tcgcgccggt aaaaatccac gcgctggatc
    31141 tgactcgcgg cgagctgaaa gtcgaaaata aactgtggtt taccacgctt gatgactaca
    31201 ccctgcacgc agaggtgcgc gccgaaggtg aaacgctcgc gacgcagcag attaaactgc
    31261 gcgacgttgc gccgaacagc gaagccccct tgcagatcac gctgccgcaa ctggacgctc
    31321 gtgaagcgtt tctcaacatt acggtgacca aagattcccg cacccgctac agcgaagccg
    31381 ggcattctat cgccacttat cagttcccgc tgaaggaaaa caccgcgcag cctgtacctt
    31441 tcgcaccgaa taacgcccgt ccgctgacgc tggaagacga tcgtttgagc tgtactgttc
    31501 gcggctacaa ctttgcgatc accttctcaa aaacgagtgg taaaccgaca tcctggcagg
    31561 tgaatggcga gtcgctgcta acccgcgagc caaaaatcaa cttcttcaag ccaatgatcg
    31621 acaaccacaa gcaggagtac gaagggctgt ggcagccgaa tcatttgcag atcatgcagg
    31681 aacatctgcg cgactttgcc gtagaacaga gcgatggtga agtgttgatc atcagccgca
    31741 cggttattgc cccaccggtg tttgacttcg ggatgcgctg cacctacatc tggcgcatcg
    31801 ctgccgatgg tcaggttaat gtggcgcttt ccggcgaacg ttacggcgac tatccgcaca
    31861 tcattccgtg catcggtttc accatgggga ttaacggcga acacgaccag gtggcgtatt
    31921 acggtcgtgg accgggcgaa aactacgccg acagccagca ggctaacatc atcgatatct
    31981 ggcgcagcac cgtcgatgcc atgttcgaga actatccctt cccacagaac aacggcaacc
    32041 atcagcatgt ccgctggacg gcactgacta accgccacgg caatggtctg ctggttgttc
    32101 cgcagcgccc cattaacttc agcgcctggc gctataccca ggaaaacatc cacgctgccc
    32161 agcactgtaa cgagttgcag cgcagtgatg acatcaccct gaatctcgac caccagctgc
    32221 ttggcctcgg ctccaactcc tggggcagcg aggtgctgga ctcctggcgc gtctggttcc
    32281 gtgacttcag ctacggcttt acgttgctgc cggtttctgg cggagaagct accgcgcaaa
    32341 gcctggcgtc gtatgagttc ggcgcagggt tcttttccac gaatttgcac agcgagaata
    32401 agcaatgagg atcatcgata acttagaaca gttccgccag atttacgcct ctggcaagaa
    32461 gtggcaacgc tgcgttgaag cgattgaaaa tatcgacaac attcagcctg gcgtcgccca
    32521 ctccatcggt gactcattga cttaccgcgt ggagacggac tccgcgaccg atgcgctatt
    32581 taccgggcat cgacgctatt ttgaagtgca ttactacctg caagggcagc aaaaaattga
    32641 atatgcgccg aaagagacat tacaggtcgt ggaatattat cgtgatgaaa ctgaccgtga
    32701 atatttaaaa ggctgcggag aaaccgtaga ggtccacgaa gggcaaatcg ttatttacga
    32761 tatccatgaa gcatatcggt ttatctgcaa taacgcggtc aaaaaagtgg ttctcaaagt
    32821 caccatcgaa gatggttatt tccataacaa ataacaacta cggcggcaaa aggagtttgc
    32881 cgccaccgct accctactca ttttcggaga tgtgttatgt ctgataccaa acgtaataca
    32941 atcggcaaat tcggcttgct ctcgctgact tttgccgccg tttacagctt taacaacgtt
    33001 atcaataata atattgagct tggactggcc tcggcaccga tgtttttcct cgcgacgatt
    33061 ttttatttta ttcccttctg tctgatcatc gcagaatttg tttcgttaaa taaaaactca
    33121 gaagccggtg tctacgcgtg ggtaaaaagt tcgctgggcg ggcgttgggc atttattact
    33181 gcctatacct actggttcgt aaacctgttc tttttcacct cactgttgcc gcgcgttatt
    33241 gcttatgctt cgtatgcctt cctcggctac gaatatatta tgacgccggt tgccaccacc
    33301 attatcagta tggtgctgtt cgccttctcc acctgggttt ccaccaacgg ggcgaaaatg
    33361 ctggggccaa ttacctccgt cacttcaacg ctgatgctgc tgttaacgct ctcctacatt
    33421 ttactggcag gtacggcact ggttggcggc gtacagcctg ccgatccgat caccgttgac
    33481 gcgatgatcc cgaacttcaa ctgggcgttc ctcggcgtta ccacctggat ctttatggcc
    33541 gcaggtggcg cggagtccgt cgcggtgtac gttaacgacg tcaaaggcgg ttcgaaatcg
    33601 ttcgttaaag tgatcatcct cgccgggatt tttatcggcg tactgtattc cgtttcctcg
    33661 gtgctgatta acgtcttcgt cagcagcaaa gagttgaaat ttactggcgg atcggtgcag
    33721 gtattccacg gcatggcggc gtattttggt ctgccggaag cgttgatgaa tcgctttgtc
    33781 ggtctggtgt cctttaccgc gatgttcggt tccctgctga tgtggaccgc aacgccggtg
    33841 aaaattttct tctccgaaat cccggaaggc atctttggta agaaaaccgt cgaactgaac
    33901 gaaaacggcg ttccggcgcg cgcagcgtgg atccagttcc tgatcgtcat cccgctgatg
    33961 attatcccga tgctcggttc caataccgtg caagatctga tgaataccat tattaatatg
    34021 accgccgcag cgtccatgct tccgccgtta ttcatcatgc tggcttacct gaatttacgc
    34081 gccaaattag atcacctgcc acgcgatttc cgtatgggct cccgccgcac cggtattatc
    34141 gttgtttcaa tgctgattgc gatatttgcc gtagggtttg tcgcttcgac attcccgact
    34201 ggcgcgaata ttctgaccat cattttttat aacgtcggcg gtattgttat cttcctcggc
    34261 tttgcgtggt ggaaatacag taaatatata aagggattaa cggctgaaga gcgccatatt
    34321 gaagcgacgc cagccagcaa tgttgattaa gcagaataat aaaaaatgag caggcatttt
    34381 ccctcttcct ggatggggag agggaaaata gtttctgcct ttatattttt ataattacaa
    34441 cgataaaagg ctgtactttt tctttagctc atggattaac acaatgaaat taatcactgc
    34501 accatgcaga gcattacttg cgctgccgtt ttgctacgcc ttttctgtga ccgctgatga
    34561 agcacgtccg gcagaacatg acgacacaaa aacccccgca atgacctcga catcttctcc
    34621 ttcatttcgt ttttacggcg aattaggggt tggtgggtat atggatttag agggtgagaa
    34681 taaacataaa tacagcgacg gcacctatat tgaaggtggc ctggagatga agtacggatc
    34741 ctggttcggc ctgatttacg gcgaaggctg gaccgtgcag gccgaccacg acggtaatgc
    34801 ctgggtgcct gaccatagct ggggcggttt cgaaggtggg attaaccgtt tctatggcgg
    34861 ttatcgtacc aatgatggca ccgaaatcat gctcagtctg cgtcaggatt cctcgctgga
    34921 tgacctgcaa tggtggggcg atttcacgcc cgatctgggc tacgtcattc ccaatacccg
    34981 cgacattatg accgcgctga aggtgcagaa cttaagcggc aactttcgtt atagcgttac
    35041 cgccacacct gccggacatc atgacgaaag caaagcctgg ctacattttg gcaagtacga
    35101 tcgctacgac gacaaataca cctatccggc aatgatgaac ggttacatcc agtatgacct
    35161 tgccgaaggc atcacctgga tgaacggtct ggaaatcacc gacggcacag ggcaactcta
    35221 tctgacgggc ctgcttaccc ctaactttgc cgctcgcgcc tggcaccata ccggacgcgc
    35281 cgacgggctg gacgtaccgg gaagtgaaag cgggataatg gcgagcgcca tgtatgaagc
    35341 gttaaaaggc ttttatctct ccaccgctta cacctacgcc aaacatcgcc ctgaccacgc
    35401 tgacgatgaa accacctctt tcatgcagtt tggtatctgg tacgaatacg gcggcggacg
    35461 tttcgcaacg gcttttgata gccgcttcta catgaaaaat gcctctcacg atcccagcga
    35521 ccaactcttc ctgatgcaat atttctactg gtaataagga ctgtaatttc atgaaaatca
    35581 aaactatttt aacgccagta acctgcgctc tgctgataag tttttccgcc catgccgctg
    35641 acgccgacaa ttataaaaac gtgattaacc gtactggcgc gccgcagtac atgaaggatt
    35701 acgattacga cgatcaccag cgttttaatc cgtttttcga tctcggagcc tggcatggtc
    35761 atctgttgcc agacggcccg aacaccatgg gcggctttcc gggcgttgcg ctgctgacgg
    35821 aagagtacat caactttatg gccagcaatt tcgatcgtct gaccgtctgg caagacggca
    35881 agaaagtcga cttcacgctg gaggcataca gtattcccgg cgcgctggtg caaaaactga
    35941 cagcaaaaga tgtgctggtc gaaatgactc ttcgcttcgc cacgccgcgc acgtcactac
    36001 tggaaactaa aatcatcagc aataaaccgc tggatctggt gtgggacggc gagctgctgg
    36061 aaaaactgga agcgaaagaa gggaaaccac tttccgataa aaccattgct ggcgaatacc
    36121 ctgactatca gcgcaaaatc agcgccaccc gtgatggcct gaaagtcacc tttggcaaag
    36181 tgcgcgccac ctgggatctg ctgacctccg gagaatcaga atatcaggtg cataaatccc
    36241 tgccagtgca gactgaaatc aacggcaatc gctttaccag taaggcgcat atcaacggtt
    36301 cgacgacgct ctataccacc tactcccatc tgctgaccgc tcaggaagtt agcaaagagc
    36361 aaatgcagat ccgcgatatt ctggcgcgtc cggcgtttta tctcaccgcc tcgcagcaac
    36421 gctgggaaga gtatctgaag aaagggttaa ccaatccgga tgcgacgccg gaacagacgc
    36481 gcgtcgcggt gaaagccatc gaaacgctca acggtaactg gcgatcgcct ggcggcgcgg
    36541 tgaaatataa caccgtcacg ccgtcggtaa ccgggcgctg gttctccggc aatcagacct
    36601 ggccgtggga tacctggaag caggcatttg cgatggcgca tttcaatccg gatatcgcca
    36661 aagagaatat ccgcgcggtc ttctcctggc agatccagcc aggcgacagc gtgcgtccgc
    36721 aggatgtggg ctttgtcccc gacctgatag cgtggaacct tagcaccgag cgtggcggcg
    36781 atggcggcaa ctggaacgaa cgtaacacca aacccagcct tgccgcctgg tcggtgatgg
    36841 aagtgtacaa cgtcacccag gataaaacct ggctggcaga gatgtacccg aaactggtgg
    36901 cctatcacga ctggtggcta cgtaaccgcg atcacaacgg caacggcgta ccggaatatg
    36961 gcgcaacccg cgataaagcc cacaatactg ataatggcga gatgctgttt gcagtgaaaa
    37021 aaggcaataa agaagagacg cagtctgggc tgaacaacta cgcccgcgtg gtggagaaag
    37081 gccagtacga cagtctggaa attccggcac aggttgcggc gtcgtgggaa tcggggcgtg
    37141 atgatgccgc cgtctttggg tttatcgaca aagaacagtt ggataaatat gtcgctaacg
    37201 gcggcaaacg tagcgactgg acggtgaaat tcgccgagaa ccgcagtcag gacggaacgt
    37261 tgctgggcta ctcgctattg caggagtcgg tggatcaggc cagctatatg tacagcgata
    37321 accattatct ggcggagatg gcgacgattc tcggtaagcc ggaagaggcc aaacgctatc
    37381 gtcagttagc acaacagctc gcggactaca tcaacacttg tatgttcgac ccgactacac
    37441 agttctacta tgacgtgcgt attgaagata aaccgctggc gaacggctgc gcgggcaaac
    37501 cgattgtcga gcgcggtaaa gggccggaag gctggtcgcc gctgtttaac ggtgcggcaa
    37561 cgcaggccaa tgccgacgcg gtggtgaagg tgatgctgga tccgaaagaa ttcaacacct
    37621 ttgtcccgct ggggacggcg gcgttaacca atccggcttt tggcgctgat atctactggc
    37681 gcgggcgcgt atgggtggat cagttctggt ttggtctgaa agggatggag cgttacggtt
    37741 atcgtgatga tgccctgaag ctggcggata cgttcttccg gcacgccaaa ggattaaccg
    37801 ccgatggccc aattcaggag aattacaacc cgctcactgg cgcgcagcaa ggcgcaccaa
    37861 atttctcctg gagtgccgcg catttgtata tgttgtataa cgattttttt cgtaagcagt
    37921 aattagcaac ggctggcgga tgcggcgtaa acgccttatc cgccctacat gtgcaatccc
    37981 ccaggccgga taagacgcgt caagcgtcgc atccggcgtt accaccgggc gtattctttt
    38041 tgaatcccat cacaaacccc gcactcccct tttccctttt ctccggcgac ggctaaatta
    38101 gaactcatcc gaccacataa caattatttt acataatgga caattttatg agctacccgt
    38161 cgctgttcgc cccgctggat ttaggtttta ccacgttaaa aaaccgcgtg ttgatgggct
    38221 caatgcacac cgggctggag gaatacccga acggcgctga acggctggca gcgttttatg
    38281 ccgaacgcgc ccgtcacggc gtggcgttga ttgtcagcgg cggtatcgca ccagatttaa
    38341 caggcgttgg catggaaggc ggtgcaatgc tcaatgacgc caaccagatc ccacaccatc
    38401 gcaccattac cgaagccgta catcaggaag gcggcaaaat cgccctgcaa attttgcata
    38461 ccgggcgcta cagctaccaa ccgcatctgg tcgctccctc cgcattgcag gcccccatca
    38521 accgtttcgt tcctcacgaa ctcacccatg aagagatcct gcaactgatc gacgatttcg
    38581 cccactgcgc gcaattggca cgggaggcag gatacgacgg tgtagaggtg atggggtccg
    38641 aagggtattt gatcaacgaa tttcttaccc tgcgcaccaa ccagcgtagc gaccagtggg
    38701 gcggcgatta ccgcaaccgg atgcggtttg ccgtcgaagt agtgcgtgcg gtgcgcgaac
    38761 gcgtcggcaa cgacttcatt attatctacc gactgtcgat gctcgacctg gtcgaaggcg
    38821 gcggtacttt tgccgaaacg gtagagctgg cgcaggccat tgaagcggcg ggcgcaacga
    38881 ttatcaacac cggcattggc tggcatgaag cgcggattcc gaccattgcc acgcccgtgc
    38941 cgcgcggtgc ctttagctgg gtcacgcgca aactgaaagg ccacgtttcg ctgccgctgg
    39001 tgaccaccaa ccggattaac gatccgcagg ttgccgacga tattctctcg cgcggcgatg
    39061 ccgatatggt atcgatggcg cgaccgtttc ttgctgatgc ggaactgctg tcaaaagcgc
    39121 aatcgggacg agccgatgag atcaacactt gtattggctg caaccaggcc tgtctcgatc
    39181 agatcttcgt tggcaaagtc acctcgtgcc tggtgaatcc tcgcgcctgc catgaaacca
    39241 aaatgccaat ccttcctgcc gtgcagaaaa aaaatctggc ggtggtcggt gcgggacctg
    39301 ctgggctggc gtttgccatt aacgcggcgg cgcgtggtca tcaggtaaca ttgtttgatg
    39361 ctcacagtga gatcggcggt cagtttaata tcgccaaaca gatccctggc aaagaggagt
    39421 tttacgaaac gctgcgctat taccgccgga tgatcgaagt gacgggcgtg acgctgaaac
    39481 tcaatcacac cgtgacggcg gatcagttac agtcgttcga tgaaacgatc ctcgctagcg
    39541 ggatcgtacc gcgcattccg cctatcgacg gaatcgatca tccgaaggtg ttgagttatc
    39601 tcgacgtact gcgcgacaaa gcgcctgtgg gcaaaaaggt agcaatcatc ggttgtggcg
    39661 ggattggttt tgatacggcg atgtttttaa gtcagccggg tgagtccacc agccagaaca
    39721 ttgccgagtt ctgtaatgaa tgggggatcg acagcagcct gcaacaggct ggtggcttaa
    39781 gcccgcaggg aatgcagatc ccgcgtagcc cacggcagat tgtgatgctc cagcgcaaag
    39841 ccagcaaacc gggacagggg ttaggcaaaa ccaccggctg gatccatcgc accaccctgc
    39901 tctcgcgcgg cgtgaaaatg ctcccaggcg taagttatca gaagatcgac gatgacgggc
    39961 tgcatgtggt gatcaacggc gaaccgcagg tattagcagt ggacaatgtg gtgatctgcg
    40021 cagggcaaga gccaaaccgc gcgctggcgc aaccgctgat tgatagcggg aaaactgtgc
    40081 atttaattgg cggctgcgat gtagctatgg agctagacgc acgacgggca attgcccagg
    40141 gaacacggct ggcgctggag atttaaatcg ttatttgaag cgccggatgc gacgcatccg
    40201 gcacgtcgcg ttaatcaata aacaaggcgg gagaaatgcc gaatcgcgct gccagttttt
    40261 tagcgtgttc cagcgttaat ttcctcttcc cgctcaaaac gcgtgacacc atcgatttac
    40321 tgccaatttc cggcagatcg gaaagggtta aaccatattg atccataagg gtacgaatca
    40381 cggctatacc gctaggcatg gcttgagcca tggcattaaa ttccgcaaat tcgggcgctg
    40441 attcttccca cgcggttatt ttggcacaca ccagatccag caaggggttt tcaggatcgt
    40501 tgagcagcag atgatctacc agttccagcg cctgggtgta ttgttcctcg ttctgaatac
    40561 ccgccagaaa aggtgccaca gcagttaact tttctcctgc ttgcaagatg tcggcaatcg
    40621 caatcatttt ttccccttag aacgatgaac ggcggtaaag aaatcgtatt ctttatgcgt
    40681 cataacttca cgtatgtagc ataaacgttg aacaacgcta aacaagagaa tgtagaggat
    40741 ggtgacaggg attttttgtt ttatggaagc gcgattccag aactgggcag acattgccgg
    40801 atgcgacgtt tgccgcgtct tatccgacct gggggtaatg tgctgaacgt atgccggata
    40861 aggcgtttac gccgcatccg gcaggtaacc cccaatctta ccgacgacgc cccagcttca
    40921 ctgctttcag caccacaaat ttattattcg tggcgatggt ggtgcagttg ccgaaaattt
    40981 tcttcagttt atggaagtaa tccaggtgac ggttggcaac gatatacagc tcgccgttga
    41041 ttttcaggca gcggcgggca tggtggaaca tctcccaggc gacgttatcg gtcagcgcat
    41101 gttgctgatg aaacggcggg ttgcagagca cagcattaaa acggaaaggc tccacgccgg
    41161 agagcgcgtt gttgatcata aactcgcagc gatccaacgc ctcgggcatg ttggtttcaa
    41221 cgttcaaacg gctggaagca accgccatcg gtgattcatc gacaaacacc actttcgcct
    41281 gcgggttttt atcgagcaat gtcaggccaa taacgccatt accgcaaccg agatcgacaa
    41341 tctcaccttc gagattctct ggcagatgtt gcataaagaa gcgcgcgcca atatccagcc
    41401 cagtgcggga gaaaacattc gcatggttgt ggatagtcca gtcagtgcct tccagcttcc
    41461 agctaacggt ctgcggcgca tcggccaacg gcggttcgtt gaaggtgcag ttaatcaggc
    41521 gcgctttctt ccatgccagc gtggtggtgg tcggaccgag cactttttcg aacaattcca
    41581 gcgtggaagt gtgaatgtca cgggctttgg caccggcaat aatgcgcgta tctggcgtga
    41641 ccactttgcg cagcgcgcgc agttgttgtt ccagcaatgc cagtgttttc ggcactttga
    41701 tcagcaccac acccggctgt tgcgggtagt cggcggtgct gtcgaggaac ttcacgctcg
    41761 gctcatcaat gccgttgaga cgtaaattct cacgcgtcgc cagctcgctg atgtatgagt
    41821 caccaatgct gtagggctta tgttccgcca gcacacagct taacgcacca aaagcatcat
    41881 tcaggatcaa caccgggccg cggatttctg tgtcgtccaa ctgttgcagc aaatactcat
    41941 ccgccgcttc ccacgcctgt agcgggttaa cgtcatccgt cgccggaaaa cgttgtagtg
    42001 tcagtgaacg gaaaccgttg tctaagtggc tcatcggccc tcctgaatga taaaatttcg
    42061 acgttgcccc tgaaaagggt gcgtgagtat accctttttc tctttttcgt gggcggttat
    42121 gagcaatctt acttatcttc agggttatcc cgagcagcta ctttcccagg tgcgaacgct
    42181 gattaacgaa cagcgtctgg gcgatgtgct ggcaaaacgt tatccgggaa cacacgacta
    42241 cgccactgat aaagccctct ggcaatatac tcaggatctt aaaaatcagt ttctgcgcaa
    42301 tgccccgccg atcaataaag tgatgtatga caacaagatc catgtgctga aaaacgcgct
    42361 cggcttacat actgccgttt cgcgtgtgca gggcggcaag ctgaaagcaa aagcagagat
    42421 ccgcgtcgct accgtgtttc gcaacgcgcc ggaaccgttt ttgcgcatga tcgtggtaca
    42481 cgagctggcg cacctgaagg agaaagagca taacaaagcg ttttaccagt tgtgctgtca
    42541 tatggaaccg cagtaccacc agcttgagtt cgacacccgg ctgtggctaa cgcagttgtc
    42601 gcttgggcag gacaaaatct gaaaatgatg tcaggcgtta ttttttcctc ctttagcgcc
    42661 taaaatcgac ctcctccctt tcgttaagga taacgaccat gctgcgcgca tttgcccgcc
    42721 ttcttctccg tatttgtttt tctcgccgga ctcttaaaat tggctgttta ttgttgctta
    42781 tagcaggagc aacccttttt attgccgatc gcgttatggt caacgccagc caacaactga
    42841 cctggagcga tgtcaacgcc gttccggcgc gtaacgtggg tttattgctg ggggcgaggc
    42901 cgggtaatcg ctactttacc cgacgtattg ataccgcagc agagttgtac cacgccggaa
    42961 aagtgaaatg gctattggtg agcggcgata atggacgtaa aaattacgat gaagcgtcag
    43021 gaatgcagca agccttgatc gctaaaggcg taccggcaaa agccatcttc tgcgactatg
    43081 ccggattctc aacgcttgat tcggtggtgc gtgccaacaa ggtgtttagt gaaaaccata
    43141 tcaccattat ctcgcaagaa tttcataacc aacgcgccat ctggctggca aaacagtatg
    43201 gtatcgatgc tatcggtttt aacgcgccag acctgaatat gaagcacggt ttatatactc
    43261 aactgcggga aaaacttgcc agagtcagcg cggtgattga tgcgaaaatc ctccatcgcc
    43321 agccgaaata tctcgggccg ttggttatga ttgggccgtt tagtgagcat ggctgtccgg
    43381 caaaagaata atgcgtatct gcgcacgtcg aagataaaaa aggcgtgcta cagtgacgag
    43441 agaatccctt tatggagtat ccacgcgtta tgatacgttt cgctgtgatt ggtacgaact
    43501 ggatcactcg ccagttcgtc gaggccgccc atgagagcgg taaatacaag ttaaccgccg
    43561 tttattcccg cagtcttgaa caggcccagc acttcgccaa tgatttttct gtcgagcatc
    43621 tgtttacgtc gctggaagcg atggcagaaa gcgatgccat tgacgcggtg tatattgcca
    43681 gcccgaattc cctgcacttt tcccagacac aacttttcct tagtcacaaa attcatgtga
    43741 tttgcgagaa accgctggcg tcgaatctgg cggaagtgga tgccgccatt gcctgtgcgc
    43801 gggaaaatca ggtggtgctg tttgaggcat ttaaaacggc ctacctgccg aactttcatc
    43861 tgttacgcca ggcgctgccg aaagtcggca aactgcgtaa agtctttttc aactattgcc
    43921 agtattcctc gcggtatcag cgttacctgg acggagagaa tcccaacacc ttcaatccgg
    43981 cattctccaa cggttcgatt atggatatcg gcttttactg cctggcgtcg gcggtggcat
    44041 tattcggtga accgaaaagc gtgcaggcaa ccgccagttt gctggcaagt ggtgtggatg
    44101 cccacggcgt ggtggtgatg gattacggtg atttcagcgt tacgttgcag cactcaaaag
    44161 tgagcgattc tgtcctggca agcgagattc agggcgaagc gggagcgctg gtgattgaaa
    44221 aactctctga atgccagaaa gtctgctttg taccgcgtgg cagccagatg caggatctca
    44281 cccagccaca gcatattaat accatgctct acgaagcaga gttgttcgct accctggtgg
    44341 atgagcatct ggtgaatcat ccagggctgg cggtcagtcg tatcaccgcc aaactgctga
    44401 ctgagatccg ccgccagact ggggtgattt ttccggcaga taacgtaaaa ctataattgc
    44461 caaagtaaaa cagtgtaaaa ggtatgtaac agaccattga ctgactgaat ggtctgtcat
    44521 actttgttac ctgcaaaggg gagtaacttc attgtcggtc gatcgtcatt acgatgtgtg
    44581 aaaaaccaca tccggtcacc gggcaacccg aaaggaatac gcagacgtat tccttttttg
    44641 ttgtaagtga gaccttgccg gaaggcgagg tctatgcata aaaagcagcg gctgacgtct
    44701 tccgacgttg gccgtttttt tatgtgtaag gaacttctat gaatactgtc ggcacgccgt
    44761 tgctatgggg cggattcgct gttgttgtca ccattatgct ggctatcgac ctgttgttgc
    44821 aggggcgtcg tggggcacat gccatgacca tgaaacaggc tgcggcctgg tcgctggtct
    44881 gggtgacgct gtcgttactg tttaacgccg ctttctggtg gtatctggta cagacacagg
    44941 ggcgcgaagt cgctgacccg caggcgctgg ccttcctcac cggttatctg attgagaaat
    45001 cgctggcggt cgataacgtc tttgtctggc tgatgttgtt cagctatttc tctgttccgg
    45061 cggcattaca acgtcgcgtg ctggtgtatg gcgtgcttgg ggcgattgtt ctgcgtacca
    45121 tcatgatctt caccggtagc tggctgattt cacagttcga ctggatcttg tatatcttcg
    45181 gcgcattcct gctgtttacc ggcgtgaaga tggcgctcgc tcatgaagat gaatcaggca
    45241 ttggcgacaa gccgctggtg cgctggctac gcggtcattt gcgcatgacc gacaccatcg
    45301 acaacgagca tttctttgtg cgtaaaaatg gtttgttgta tgccacgccg ctgatgctgg
    45361 tgctgattct ggtggagttg agcgacgtga ttttcgccgt ggatagtatt ccggctatct
    45421 tcgccgtgac caccgatccg tttattgtgc tgacctcaaa cctgtttgcg atcctcggcc
    45481 tgcgtgcgat gtatttcctg ctggcaggtg tggcagagcg tttctcgatg ctcaaatatg
    45541 gtctggcggt gattctggtg tttatcggta tcaagatgct gattgtcgac ttctaccata
    45601 ttccgatcgc cgtctcgctg ggcgtggtgt ttggcattct ggtgatgacg tttattatca
    45661 acgcctgggt gaattatcgg catgataagc agcgggtaga ataattttta atctgcctaa
    45721 gccgtgtact ctgtcatcaa catgagcacc gttttctccc tctccccaaa aggggcgagg
    45781 ggactgaccg agtacttttt tgacgttgtc atcagtctgg aagccgcacg ttgggcttta
    45841 ttttcatgtc aaacaaatgt aaccattaag ttgcgaaata tgacttctct ttaaaatcca
    45901 gcatatttcg cttcccgaag ctgtaacttt ccttatactc gaccttgcaa acactttgtt
    45961 acatcctgaa agatgcgtcg acagaacgca ccagggatgt gcgacaacac aatgaaagga
    46021 tcgaaaaatg actacgcaac attcaccggg gctattccgg cgtctggctc atggcagcct
    46081 ggtaaaacaa atcctggtcg gccttattct ggggattctt ctggcatgga tctcaaaacc
    46141 cgcggcggaa gctgttggtc tgttaggtac tttgttcgtc ggcgcactga aagccgttgc
    46201 ccccatcctg gtgttgatgc tggtaatggc atctattgct aaccaccagc acgggcagaa
    46261 aaccaatatc cgccctattt tgttcctcta tctgctgggc accttctctg ctgctctggc
    46321 cgcagtaatc ttcagttttg ccttcccttc taccctgcac ttgtccagta gcgcgggtga
    46381 tatttcgccg ccgtcaggca ttgtagaagt gatgcgcggg ctggtaatga gcatggtttc
    46441 caaccccatt gacgcgctgc tgaaaggtaa ctatatcggg atcctggtgt gggcaattgg
    46501 cctcggtttc gcactgcgtc acggtaacga aaccaccaaa aacctggtca acgatatgtc
    46561 gaatgccgtt acctttatgg tgaaactggt gattcgcttc gcaccgatcg gtatttttgg
    46621 tctggtttct tctaccctgg caaccaccgg tttctccacg ctgtggggct acgcgcaact
    46681 gctggtggta ctggttggct gtatgctgct ggtggcgctg gtggttaacc cactgctggt
    46741 atggtggaaa attcgtcgta acccgttccc gctggtgctg ctgtgcctgc gcgaaagcgg
    46801 cgtgtatgcc ttcttcaccc gcagctctgc ggcgaacatt ccggtgaata tggcgctgtg
    46861 tgaaaagctg aatctggatc gcgataccta ttccgtttct attccgctgg gagccaccat
    46921 caatatggca ggcgcagcaa tcaccattac cgtgttgacg ctggctgcgg ttaatacgct
    46981 gggtattccg gttgatctgc ccacagcgct gctgttgagc gtagtggctt ctctgtgtgc
    47041 ctgtggcgca tccggcgtgg cgggggggtc tctgttgctg atcccactgg cctgtaatat
    47101 gttcggtatt tcgaacgata tcgccatgca ggtggttgct gtcggcttta ttatcggcgt
    47161 attgcaggac tcctgtgaaa ccgcgctgaa ctcgtcaact gacgtgctgt tcactgcggc
    47221 agcttgccag gcggaagacg atcgtctggc aaatagcgcc ctgcgtaact aatacttagc
    47281 ccctttcgtc tacggcggaa ggggttttct ccactttaaa cggatcaatt ccccttctct
    47341 gcatacgcca gaaacgaatg atattcaggc cattcatcag cagaaaactc ccctcaatca
    47401 tcgtgccgcc tatcgacccc gcccagaagt tgtgaatcac ccagcaacac gttgaaaacc
    47461 acattacgca gcgcatggtc agccctttac agcggaacag cgcccaggta ctgacaatcg
    47521 tgccgattac cggcaatagt tcgacaggat gatggaactt cgcgaggcca attccgccag
    47581 tcagcacaat aaaaatcgcc attacccata agctgcgcgt gcgtaaggta atcaatgtac
    47641 gaatggcatt aaggatggca ctggcaccgg caggataggt gcccagaaga aaaaaatgta
    47701 cgccaataac ggcgctatag accgaaagtt gctttttgaa gcgacgttcg tcacgattga
    47761 aaaatgttgt gataccaatc agaaaggcga tgacacccac gccctgggcc agccaatacg
    47821 cggtcatgat aaatccttag caggtatgga aaagcaaacg gcgcttcaca ttatgaaacg
    47881 ccgtttttta ttaacaactc atttcgactt tatagcgtta cgccgctttt gaagatcgcc
    47941 agttcgcgga agtcgttacg ctcgttacag gtttgcttac cgttggcaaa ctcaacgatg
    48001 gtgtcgataa attcttccag caactgcggc atcgctttac cgtggatcag ctgacccgcg
    48061 tcaaagtcga tccagtgttt tttcttcgcc gccagttcgc tgttggtggc gattttcacc
    48121 gtcggtacaa agccgccgta cggcgtgccg cgaccagtac tgaacagcac catatggcag
    48181 cccgcacccg ccagggcgct ggtcgctacg gcatcgttac ccggcgcact taacaagttc
    48241 agccccggcg ttttcagacg ctcgccgtaa cgcagcacgt caaccacgac actggaaccc
    48301 gctttctggg tacagccaag cgatttatct tccagcgtgg tgataccgcc tgctttgttc
    48361 cccggtgatg ggttttcgta gatcggctgg tcatgggcaa taaagtactg cttgaagtca
    48421 ttgaccatgg tgaccagttt ttcaaacgtt gcttcgtcgc gacaatggtc catcagcaac
    48481 tgctctgcgc caaacatctc cggcacttcg gtcagtacgg tagtaccgcc gttagcaatc
    48541 acgtagtcag agaaacgccc cagcatcggg ttagcagtaa taccagaaag accgtcagaa
    48601 ccaccgcact ccagaccaaa cttcagttcg ctgagtttgc ccggctcgcg tttatcgttg
    48661 cgcatcacgc tatacagctg atgcaaatgc gcgatcccgg cctcgatttc atcatcctgc
    48721 tgttggcaga tcatgaaatg aacgcgttca ggatcgatat cgcccaacgt ttcacggaat
    48781 gcggcaacct ggttattttc gcaacccaga ccaatcacca gcactgcgcc cgcgttcggg
    48841 tggcgcacca tgttttgcag catggtgcga gtattgatgt gatcgtcgcc cagttgtgag
    48901 cagccgtagg tgtggctgaa gaggaacacg ccgtcggtac cttcagcgtt gttggtctct
    48961 ttcaggaaac gattttggat ctgccgcgcg atgccgttga cacagcccac ggttggcagg
    49021 atccacagtt cattacgcac cccgacatcg ccgttagcgc gacgatagat ctgcacttca
    49081 cgatctgccg cttgcgcagg cagatcctga aaatcaggtt gatagcgata ctgatccaga
    49141 tcgctcagat tcgtgcgcgt attgtgggcg tgaacgtgtt cccccgccgc aatatccgcc
    49201 aatgcataac caatcggcag gccatattta atgacgttgg ccccttttgc gatatccgtt
    49261 aacgcaaatt tatgtccacg agcaacatcc tggcgcagcg taacagtctg gttatcgaca
    49321 ctgacttctg tgccttcagc caaatccgct aaagcaaccg cgacgttatc cagcgcatgg
    49381 atcttgatgt attgcatatc aaccccagac cttagttcag ttcaatggcg aagtagtcac
    49441 gcgcattgtt aaagcaaata tttttcacca tctcgcccag caggttgatg tccgccggtg
    49501 cttcgcctgc ttccacccag cgaccgatca tctggcacag aatgcggcgg aagtattcgt
    49561 gacgggtgta tgacaggaag ctacggctgt cagtcagcat gccgacaaag cggctcagca
    49621 gaccgagctg cgccagttgg gtcatctgac gttccatacc gtctttctgg tcgttaaacc
    49681 accagccgga accgaactgc attttgcccg gcataccttc gccctggaag ttaccgatca
    49741 tggtgcccag cacttcgtta tcgcgcgggt tcaggcagta gagaatggtt ttcggcagca
    49801 ggttttcttc attctgcttg ctcagcagct tagacagctc ttccgccatc ggacggtcgt
    49861 tgatggagtc aaagccgaca tccggcccca gcagtttaaa ctgacgcagg ttgttattac
    49921 gcagtgcgcc aatgtggtac tgctgtaccc agccgcgacg tgcatattcg gcaccaagga
    49981 acaccagcac cgcagttttg aactgtgcca cttcgtgctc gctcagggtt tcgcctgcca
    50041 gacggcgcgc gaggatgctg tccagttccg cttcgttcgc ttcagcaaac atcaccacat
    50101 ccagcgcgtg gtcagacact ttacagccgt gagcggcgaa gtgatccaga cgtttagtca
    50161 gggcagtttg caggtcagca aagcggcgaa tgtcggtatc ggaaacttcg cccagcttcg
    50221 ccatgtagtc gttaaaggtc gcctgttcaa tgttaaaggc tttgtccgga cgccagctcg
    50281 gcagcacttt aatggtgaaa gagccatctt tggcgatctc tgcgtgatgc tccagagaat
    50341 cgatcgggtc atcggtggtg ccgaccattt tcacgttcat ctgctgcatg atgccgcgcg
    50401 cggagaagtt atcctgcgcc agcagttcgt tacattcgtt ccagatttca tcggcagttg
    50461 acggagaaag caatttgcca gtgataccaa acggacgacg cagttcgagg tgcgtccagt
    50521 ggtataacgg gttgccgata gtgtgcggaa cagtcgccgc ccaggcgtca aatttttcac
    50581 ggtcagacgc atcaccggtg cacagacgct cggccacacc gttggtacgc atagcgcgcc
    50641 atttgtagtg atcgcccttc aaccagatgt catacaggtt tttaaaacga tagtcttccg
    50701 caatctgctg cggcggcaaa tggcaatggt aatcgaaaat cggctggtct tttgcgtagt
    50761 cgtgatacag acggcgggca aattcggtat ctaacaggaa atcttcagtc ataaacggag
    50821 tcattttcgt cttcctctca acgagtgagc tagcttgctt atggtgcgat gctgacaaag
    50881 ttatcacacc aatttccaga gtccgaagat attttcgtga gttagatcaa taaacgtagt
    50941 taaaaaaatt actctcaaag tggtaaatct cgctgcaagc cgcgccagta ctggcctcgc
    51001 cgtcgtcagg taatgtccct acaaatattc gcacatttgt gatggctctc accttttaaa
    51061 gttgtatgac aagttatctt tctgccgtcg caaatcataa gtcgacggaa tgcaaattgc
    51121 cgattcattt gttagatgaa tcgggttaac cggtacggaa gccgaattag cacgaaactt
    51181 tcatggcaac gttcggggcg tgccggtttt ttttcggtta cccggtcgta actaacatct
    51241 tcagcctctg gcgggatgat ggccgcgctt cctgcggata taacaaaacg atgaggtttt
    51301 acatgcgtaa aattaaaggg ttacgttggt atatgatcgc actggtgacg ctcggcaccg
    51361 tgcttggtta cctgacgcgt aacactgtgg cggcagctgc gccaactctg atggaagagt
    51421 taaacatctc cacccaacag tattcctata tcatcgcagc gtattctgct gcttatacgg
    51481 tcatgcaacc ggtagcaggt tatgtactgg atgtgctggg tacgaaaatc ggttatgcaa
    51541 tgtttgctgt actgtgggcc gtgttctgtg gtgcaaccgc gctggcaggt agttggggtg
    51601 gcctggcggt tgctcgtggt gcggtcggtg ccgcggaagc cgcgatgatt ccggcgggtc
    51661 tgaaagccag ctccgaatgg ttcccggcga aagagcgttc catcgcggta ggttacttta
    51721 acgtaggttc ttcgattggt gcgatgattg cgccgccgct ggtggtatgg gcaatcgtga
    51781 tgcacagctg gcagatggca tttatcatct ccggtgcgtt gagctttatc tgggcgatgg
    51841 catggctgat tttctacaag catccgcgcg accagaaaca tctgaccgat gaagaacgcg
    51901 actacattat taatggtcag gaagcccagc accaggttga cacagcgaag aaaatgtccg
    51961 ttggtcagat cctgcgtaac cgtcagttct ggggtatcgc gctgccgcga ttcctggcag
    52021 aaccggcatg gggtactttt aacgcgtgga tcccgctgtt catgtttaaa gtttacggct
    52081 ttaacctgaa agagatcgcg atgtttgcct ggatgccgat gctgtttgct gacctcggtt
    52141 gtatcctcgg tggttacctg ccgccgctgt tccagcgttg gtttggtgtg aacctgatcg
    52201 tttcccgtaa gatggtcgta acgctgggcg ctgtgctgat gattggcccg ggtatgatcg
    52261 gtctgttcac caacccgtat gtcgcgatta tgctgctgtg tatcggtggt tttgctcacc
    52321 aggcactgtc tggcgcactg attacgctct cgtctgacgt gttcggtcgt aacgaagtgg
    52381 cgacggcgaa cggtttaacc gggatgtccg catggctggc aagtacgctg tttgcactgg
    52441 tagtcggtgc actggctgac accatcggct tcagcccgct gttcgcagtt ctggcagtgt
    52501 tcgacctgtt aggtgcgctg gttatctgga ccgtgttgca gaacaaaccg gcgattgagg
    52561 tggcgcagga atcacataac gatcccgcac cgcaacatta aggcttcgtg taagttttag
    52621 tttcccttca aagccgcctt ctcaggcggc tttttcatca ctgcgagtag agctaaactc
    52681 gcaaaagtgg tataacaaat atagtctgcc gtatcatgcc tggagcgcat atggaaatca
    52741 ctgaaccacg ccgtttgtat caacaacttg ccgctgacct gaaagagcgc atcgaacagg
    52801 gcgtctatct ggtgggtgat aaactgcctg ccgaacgctt tattgccgat gaaaaaaacg
    52861 tcagccgcac cgtggttcgt gaagccatca tcatgctgga agttgaaggc tatgtcgaag
    52921 tgcgtaaagg ttcaggtatt catgtggttt ccaaccagcc tcgccatcag caggcggctg
    52981 acaataatat ggaattcgcc aattacggtc cgtttgagtt gctccaggct cgccagctca
    53041 tcgaaagtaa tattgccgag ttcgcggcaa ctcaggtcac aaaacaggac atcatgaaat
    53101 tgatggccat tcaggagcag gcacgcggcg aacaatgctt ccgtgattcc gagtgggatt
    53161 tgcagttcca tattcaggta gccctggcga cgcagaactc cgccctggcg gctatcgttg
    53221 aaaaaatgtg gacccagcgt agtcataacc cgtactggaa aaaactgcac gaacacattg
    53281 atgcccgtac cgtcgataac tggtgtgatg accacgatca aatcctcaag gcgctgattc
    53341 gcaaagatcc tcatgctgct aagctggcaa tgtggcagca cctggaaaac accaagatca
    53401 tgttatttaa cgaaaccagc gacgatttcg agttcaatgc cgaccgctat ctgttcgccg
    53461 aaaacccggt ggtgcatctc gatacagcca ccagcggcag taaatgaaat tcctgtccga
    53521 caggcgctac agtgcgcctg tttgtctcgt cggtaagcga aaagtataaa gtgtcagcct
    53581 gtgtaaatcc tctcgcctcc ctcccctgca ttcagcaaaa tcagactcca cggacatgta
    53641 attttgataa cgaacaacgt tgacctttgt tacaattaga ttcaatttga atttatgctt
    53701 ttgaatgctt tcttatctca cgatttaaca gggaatagtt caggctgtgt tgatgtatca
    53761 aacccgcaga acataccaag acagcaataa cattgcggta gtgcatcatt taaaaccagc
    53821 gtggcgttaa ccgattcacc aggaataatg aatggaactt ttgacccaat tgctgcaagc
    53881 cctgtgggcg caggattttg aaaccctggc caatccgtcg atgattggca tgttgtattt
    53941 tgtcttgttt gtcattttgt tccttgaaaa cggcttgctt ccggcggcct ttttaccggg
    54001 cgacagttta ctggtactgg tcggcgtatt gattgcgaaa ggcgcgatgg gctatccgca
    54061 aacgattctg ctgctgaccg ttgccgccag cctcggttgc tgggtcagct atattcaggg
    54121 gcgatggctg ggcaataccc gcaccgtaca aaactggcta tctcatttac ccgcgcatta
    54181 tcatcaacgc gcacaccatc tttttcataa acacggttta tcggcgctgt taattggtcg
    54241 ctttattgcg tttgtcagaa cactgctgcc gacgattgcc gggttatcag ggctgaataa
    54301 cgcgcgcttt cagttcttca actggatgag tggtctgctg tgggtgttga tcctgacaac
    54361 tctgggttac atgctcggca aaacgccggt atttttaaag tacgaggacc agctgatgtc
    54421 atgcctgatg ctgctcccgg tggtgctgct ggtgtttggc ctggcaggtt ctctggtcgt
    54481 gttatggaaa aagaaatatg gaaatcgggg gtaagggatg caaatacctc gcatgtcact
    54541 tcgccagcta gcctggtccg gcgctgtttt acttctggtc agcacactat tgctggcctg
    54601 gtccgcggtt cgccagcaag agtctacgct ggcgattcgt gccgttcatc aagggacaac
    54661 gatgccagac ggtttttcaa tctggcatca ccttgacgct cacggcattc ctttcaaaag
    54721 tatcaccccc aaaaacgaca ctctgttaat tacctttgac tccagcgacc agagcgccgc
    54781 cgcaaaagcg gtcctcgaca gaacattgcc ccatggctac atcattgcgc agcaggacaa
    54841 taacagtcag gctatgcagt ggctgacccg gttgcgggat aactctcatc gcttcggata
    54901 acttcctgtt atccgaaaca gatcactcac tttggtgatt tcaccgtaac tgtctatgat
    54961 taatgaggcg gtggttaaca ctaccgcttc gttgtctgga caatcgttcc tttgtaatag
    55021 gtccgaatca caatggaagg ttcaagaatg aaataccgca tcgctttagc tgtttctctc
    55081 tttgctctta gtgccggtag ttatgccact accctgtgtc aggaaaagga gcaaaatatc
    55141 cttaaggaga tcagctatgc cgaaaaacac caaaaccaga atcgtattga cggtctgaat
    55201 aaagccctga gtgaagtccg ggccaactgt tcagatagcc agctgcgtgc cgatcatcag
    55261 aagaaaatcg caaagcagaa agatgaggtg gcggaacgcc agcaagattt agccgaggcg
    55321 aagcaaaaag gcgatgcaga taagattgcc aaacgcgaac ggaaactggc agaagcgcag
    55381 gaagagctga aaaagctgaa agcgcgcgac tactaactca caatagtcac tacttactca
    55441 cctggagaaa actatgtcga aagaacacac tacggaacat ctgcgtgctg agttgaaatc
    55501 cctttccgat acgctggaag aggtgcttag ctcatctggc gagaagtcaa aagaagagtt
    55561 gagtaagatt cgtaacaaag cggagcaggc actgaaacag agccgttatc gcctgggtga
    55621 aaccggtgat gccattgcca aacaaacccg tgtcgcggcg gcgcgtgccg atgagtatgt
    55681 gcgtgaaaat ccgtggacgg gcgtgggcat tggcgcagca atcggtgtag tgctcggcgt
    55741 tctgctgtcg cgtcgttaat tatggcggac actcatcacg cgcaagggcc cggtaaaagc
    55801 gttctgggca tcgggcagcg aattgtttcc atcatggttg aaatggtgga gacacgtctg
    55861 cggctggcgg tggtggagct ggaagaggaa aaagcgaatc tctttcaact tttactgatg
    55921 ctgggcctga cgatgctttt cgctgcattt ggtcttatga gcctgatggt gctaattatc
    55981 tgggcggttg atccgcaata tcgcctgaat gcgatgattg ccaccaccgt ggtgttgctg
    56041 ctactggcac tgattggcgg catctggacg ttacgtaaat cgcgcaagtc tacgttgctg
    56101 cgccacacgc gccatgagct ggcaaacgat cggcagctgc tcgaggagga gtcccgtgag
    56161 cagtaaagtc gaacgtgaac gacgtaaggc gcaactgctt agccagatcc agcaacaacg
    56221 gctggatctt tccgccactc gtcgtgaatg gctggaggca acaggcgctt acgatcgtcg
    56281 ctggaatatg ctgttgagtc tgcgctcctg ggcgctggtt ggcagtagcg tgatggctat
    56341 ctggacgatt cgccatccta atatgctggt ccgctgggcc agacgcggtt ttggtgtatg
    56401 gagcgcctgg cgtctggtga aaacaaccct caagcaacaa cagcttcgcg gttaacttcc
    56461 tctctggccg gagaaattcc ggccttatcc ctcaaatttt ttgaaaatat ttgacagttt
    56521 tccttgctaa caatcacgct tcgtcacgtt tatgattctc tccatcgaca gcaacgacgc
    56581 taatagcgcg cccttgcaca aaaaaacaat cagcagcctg agcggcgcag tggagagtat
    56641 gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcgt attttaatgc cgattctgtt
    56701 tattacggca ggctggggaa aaattactgg ctacgcgggt acccaacaat atatggaagc
    56761 aatgggcgtc cccggcttta tgctgccgct ggtgattctg cttgagtttg gtggtggtct
    56821 ggcaatcctg ttcggtttcc tgacacgtac cacagccctg tttactgcgg gctttacgct
    56881 gctgacggca tttttatttc acagcaactt tgcagaaggc atgaactcgc tgatgtttat
    56941 gaaaaacctg actatttctg gcggattcct gctgctggca attaccggtc caggcgcatt
    57001 cagcatcgac cgcctgctga ataaaaagtg gtaagcacgc gctatactta acgataaaaa
    57061 acgaggggga agctcctcgt ttttgctatt ggaggataga aaaatgggtc aactgattga
    57121 cggcgtctgg catgacacct ggtacgatac caaatccacc ggcggtaaat ttcaacgttc
    57181 agcttccgca tttcgtaact ggctcactgc cgatggcgcg ccaggcccca ctggcaaagg
    57241 cggttttgcc gcagagaaag atcgttatca tctctatgtt tcactcgcct gcccgtgggc
    57301 acaccgcacg ctgatcatgc gtaaactcaa aggactggaa ccgtttattt ccgtttccgt
    57361 agtgaacccg ctgatgctgg aaaacggctg gacctttgat gacagttttc cgggagcaac
    57421 cggcgacacg ctctatcaac atgaatttct gtatcagctt tatctccacg ccgatccaca
    57481 ctacagcgga cgagtgactg ttcccgtgct gtgggacaaa aaaaaccaca ccatcgtcag
    57541 caacgaatca gcagaaatca tacgcatgtt caataccgcg tttgatgcgc tgggtgcgaa
    57601 agctggagat tactacccac cagccctgca accgaaaatt gacgaactta acggctggat
    57661 ttacgacacc gttaacaatg gcgtgtataa agccggtttt gccaccagcc agcaagccta
    57721 cgacgaggcg gtggcgaaag tgtttgaatc gctggcgcga ctggaacaga ttttaggtca
    57781 gcaccgttac ctgaccggca accagctaac cgaagccgat attcgcctgt ggaccacgct
    57841 ggtgcgtttt gatccagtgt atgtgaccca cttcaagtgt gataagcacc gcatcagcga
    57901 ttacctgaat ctgtatggct tcctgcgcga tatctaccag atgccgggaa ttgccgaaac
    57961 agtcaatttc gatcacatcc gtaatcatta cttccgcagc cataagacca tcaaccctac
    58021 ggggattatt tcaattggtc cgtggcagga tctcgatgaa ccgcatggac gagatgttcg
    58081 cttcggttaa aaaaaggcac cttacgaggt gcctctgata attagctaag tacgtgttgt
    58141 tcatgtcgga tgcggcgtaa acgccttatc aaacctacaa aacatttata ttcaatatgt
    58201 tgcaatgacc gcgcaggcca gataagcgta gcgcatctgg caattttgcg cttgccctta
    58261 tccttcacag cgccttatta attcatcgcg ataatattta ctgccatttc attcacgtct
    58321 attcttaatt tgctgcttta aaaacaagtg attgagcaaa attgaggcaa aaatggactg
    58381 gtatctgaaa gtactaaaaa attatgtcgg tttccggggt cgtgcgcggc gcaaagagta
    58441 ctggatgttt attctggtca acatcatctt tacgttcgtg ctggggctac tggataaaat
    58501 gttaggctgg caacgtgccg gtggcgaagg catcctgacg acaatctacg gtattctggt
    58561 gtttttacca tggtgggcag ttcagttccg ccgcctgcac gacaccgacc gctcggcgtg
    58621 gtgggcactg ctattcttaa tcccgttcat cggctggctt attatcatcg tctttaactg
    58681 ccaggcgggt acgccaggcg aaaaccgctt tggaccagat ccgaaactgg aacaagaata
    58741 ataattaagc accttgtaaa aggtgcttaa tcgattattt tccgctaaaa agtttgggaa
    58801 tttcccgcag acaccaggat ttcgcctccc ccatactgtc acgacgccag gccataataa
    58861 tatcgatctc gctggtcgat tccgggctga cgacacgcaa ccgcccttcc gcaatatctt
    58921 tttcgaccat cggatacggc atcgtcgcca cgccaagccc cgccagtaat gcctgacgtt
    58981 tatcttcaat cgtgctcacc gttaagcgcg gctgtttgtc cagcaactgt acggtcaaca
    59041 ccgggcgctc acgagcggta tccgccaccg caattccacg atatttcacg cgcgtcactt
    59101 cagataacgg ttccggctcc tgatgaatcg ggtgatccgg cgcggcaaca tagacgttca
    59161 ttaaggtata gagtttgcgc gagttgatct ccgacgagga acgaaaatgc atatccggcg
    59221 cgataacgat at
//
LOCUS       DAZFJL010000011        57514 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFJL010000011 DAZFJL010000000
VERSION     DAZFJL010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.011"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            210..1322
                     /gene="potF"
                     /locus_tag="ACHJ8C_000677"
     CDS             210..1322
                     /gene="potF"
                     /locus_tag="ACHJ8C_000677"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO5550584.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVSTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSANFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            1417..2550
                     /gene="potG"
                     /locus_tag="ACHJ8C_000678"
     CDS             1417..2550
                     /gene="potG"
                     /locus_tag="ACHJ8C_000678"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO5550585.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDVDASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            2560..3504
                     /gene="potH"
                     /locus_tag="ACHJ8C_000679"
     CDS             2560..3504
                     /gene="potH"
                     /locus_tag="ACHJ8C_000679"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO5550586.1"
                     /translation="MSTLEPAAQPKPFKLWLSQLQMKHGRKLVIALPYIWLILLFLLP
                     FLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQSLQV
                     AAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKNNGV
                     LNNFLLWLGIIDQPLTILHTNFAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAALDL
                     GARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFF
                     NNRDWPVASAVAIIMLLLLIMPIMWFHKHQQKSEGEHG"
     gene            3501..4346
                     /gene="potI"
                     /locus_tag="ACHJ8C_000680"
     CDS             3501..4346
                     /gene="potI"
                     /locus_tag="ACHJ8C_000680"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO5550587.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLIIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            4406..4894
                     /gene="ybjO"
                     /locus_tag="ACHJ8C_000681"
     CDS             4406..4894
                     /gene="ybjO"
                     /locus_tag="ACHJ8C_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO5550588.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            4935..6062
                     /gene="rlmC"
                     /locus_tag="ACHJ8C_000682"
     CDS             4935..6062
                     /gene="rlmC"
                     /locus_tag="ACHJ8C_000682"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO5550589.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTVDLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(6091..6822)
                     /gene="artJ"
                     /locus_tag="ACHJ8C_000683"
     CDS             complement(6091..6822)
                     /gene="artJ"
                     /locus_tag="ACHJ8C_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO5550590.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(7048..7716)
                     /gene="artM"
                     /locus_tag="ACHJ8C_000684"
     CDS             complement(7048..7716)
                     /gene="artM"
                     /locus_tag="ACHJ8C_000684"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO5550591.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(7716..8432)
                     /gene="artQ"
                     /locus_tag="ACHJ8C_000685"
     CDS             complement(7716..8432)
                     /gene="artQ"
                     /locus_tag="ACHJ8C_000685"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO5550592.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAVIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(8439..9170)
                     /gene="artI"
                     /locus_tag="ACHJ8C_000686"
     CDS             complement(8439..9170)
                     /gene="artI"
                     /locus_tag="ACHJ8C_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO5550593.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(9188..9916)
                     /gene="artP"
                     /locus_tag="ACHJ8C_000687"
     CDS             complement(9188..9916)
                     /gene="artP"
                     /locus_tag="ACHJ8C_000687"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO5550594.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(10134..10649)
                     /gene="ybjP"
                     /locus_tag="ACHJ8C_000688"
     CDS             complement(10134..10649)
                     /gene="ybjP"
                     /locus_tag="ACHJ8C_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5550595.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRGNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            10775..11098
                     /gene="ybjQ"
                     /locus_tag="ACHJ8C_000689"
     CDS             10775..11098
                     /gene="ybjQ"
                     /locus_tag="ACHJ8C_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO5550596.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            11095..11925
                     /gene="amiD"
                     /locus_tag="ACHJ8C_000690"
     CDS             11095..11925
                     /gene="amiD"
                     /locus_tag="ACHJ8C_000690"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO5550597.1"
                     /translation="MRRVFWLVAAALLLAGCTGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYYIKP
                     ENVVAHADIAPQRKDDPGPLFPWKQLAQQGIGAWPDAQRVNFYLAGRAPHTPVETASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(11922..12935)
                     /gene="ybjS"
                     /locus_tag="ACHJ8C_000691"
     CDS             complement(11922..12935)
                     /gene="ybjS"
                     /locus_tag="ACHJ8C_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO5550598.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVLTLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(13034..14464)
                     /gene="ybjT"
                     /locus_tag="ACHJ8C_000692"
     CDS             complement(13034..14464)
                     /gene="ybjT"
                     /locus_tag="ACHJ8C_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO5550599.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQLLAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLLPIPFPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQPLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SARLQALWRVVNQIGGKERYFFGNILWQTRALMDLAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKRIARLAEQSTD"
     gene            complement(14475..15476)
                     /gene="ltaE"
                     /locus_tag="ACHJ8C_000693"
     CDS             complement(14475..15476)
                     /gene="ltaE"
                     /locus_tag="ACHJ8C_000693"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO5550600.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNATWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSRAQLAEVAAHWRAFLAR"
     gene            complement(15513..17231)
                     /gene="poxB"
                     /locus_tag="ACHJ8C_000694"
     CDS             complement(15513..17231)
                     /gene="poxB"
                     /locus_tag="ACHJ8C_000694"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO5550601.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRVFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTERKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(17364..18332)
                     /gene="hcr"
                     /locus_tag="ACHJ8C_000695"
     CDS             complement(17364..18332)
                     /gene="hcr"
                     /locus_tag="ACHJ8C_000695"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO5550602.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSRWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKTLG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVISGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(18344..19996)
                     /gene="hcp"
                     /locus_tag="ACHJ8C_000696"
                     /gene_synonym="priS"
     CDS             complement(18344..19996)
                     /gene="hcp"
                     /locus_tag="ACHJ8C_000696"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO5550603.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(20141..21040)
                     /gene="lysO"
                     /locus_tag="ACHJ8C_000697"
     CDS             complement(20141..21040)
                     /gene="lysO"
                     /locus_tag="ACHJ8C_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO5550604.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(21361..22056)
                     /gene="aqpZ"
                     /locus_tag="ACHJ8C_000698"
     CDS             complement(21361..22056)
                     /gene="aqpZ"
                     /locus_tag="ACHJ8C_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO5550605.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            22482..24140
                     /gene="ybjD"
                     /locus_tag="ACHJ8C_000699"
     CDS             22482..24140
                     /gene="ybjD"
                     /locus_tag="ACHJ8C_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO5550606.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENIPMNLRKVISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(24137..25093)
                     /gene="ybjX"
                     /locus_tag="ACHJ8C_000700"
     CDS             complement(24137..25093)
                     /gene="ybjX"
                     /locus_tag="ACHJ8C_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO5550607.1"
                     /translation="MNMSQLTERTFTSSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEITEIASKKRAEYRRRYEMLDAIQPQMATMFCS"
     gene            25244..26359
                     /gene="macA"
                     /locus_tag="ACHJ8C_000701"
     CDS             25244..26359
                     /gene="macA"
                     /locus_tag="ACHJ8C_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO5550608.1"
                     /translation="MKKRKTVKKRYVIAMVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAETELKLARVTYSRQQRLAQTQAVSQQDLDNAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKINDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            26356..28302
                     /gene="macB"
                     /locus_tag="ACHJ8C_000702"
     CDS             26356..28302
                     /gene="macB"
                     /locus_tag="ACHJ8C_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO5550609.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVHNPPAVEKVNATGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(28375..28599)
                     /gene="cspD"
                     /locus_tag="ACHJ8C_000703"
     CDS             complement(28375..28599)
                     /gene="cspD"
                     /locus_tag="ACHJ8C_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO5550610.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            28749..28823
                     /gene="yljB"
                     /locus_tag="ACHJ8C_000704"
     CDS             28749..28823
                     /gene="yljB"
                     /locus_tag="ACHJ8C_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO5550611.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            28922..29242
                     /gene="clpS"
                     /locus_tag="ACHJ8C_000705"
     CDS             28922..29242
                     /gene="clpS"
                     /locus_tag="ACHJ8C_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO5550612.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            29273..31549
                     /gene="clpA"
                     /locus_tag="ACHJ8C_000706"
     CDS             29273..31549
                     /gene="clpA"
                     /locus_tag="ACHJ8C_000706"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO5550613.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(32070..32157)
                     /locus_tag="ACHJ8C_000707"
     tRNA            complement(32070..32157)
                     /locus_tag="ACHJ8C_000707"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32121..32123),aa:Ser,seq:gga)
     gene            complement(32411..32629)
                     /gene="infA"
                     /locus_tag="ACHJ8C_000708"
     CDS             complement(32411..32629)
                     /gene="infA"
                     /locus_tag="ACHJ8C_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO5550614.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(32914..33618)
                     /gene="aat"
                     /locus_tag="ACHJ8C_000709"
     CDS             complement(32914..33618)
                     /gene="aat"
                     /locus_tag="ACHJ8C_000709"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO5550615.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRLPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCDEFIRHGGKLIDCQVLNDHTASLGACEIPRWDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(33660..35381)
                     /gene="cydC"
                     /locus_tag="ACHJ8C_000710"
     CDS             complement(33660..35381)
                     /gene="cydC"
                     /locus_tag="ACHJ8C_000710"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO5550616.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEATLRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMCEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLTRQGRY
                     YQFKQGL"
     gene            complement(35382..37148)
                     /gene="cydD"
                     /locus_tag="ACHJ8C_000711"
     CDS             complement(35382..37148)
                     /gene="cydD"
                     /locus_tag="ACHJ8C_000711"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO5550617.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIISQAWF
                     MARILQHMIMENIPREALLLPFTLLFLAFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTEPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQTALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(37271..38236)
                     /gene="trxB"
                     /locus_tag="ACHJ8C_000712"
     CDS             complement(37271..38236)
                     /gene="trxB"
                     /locus_tag="ACHJ8C_000712"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO5550618.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNCPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            38780..39274
                     /gene="lrp"
                     /locus_tag="ACHJ8C_000713"
     CDS             38780..39274
                     /gene="lrp"
                     /locus_tag="ACHJ8C_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO5550619.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            39409..43398
                     /gene="ftsK"
                     /locus_tag="ACHJ8C_000714"
     CDS             39409..43398
                     /gene="ftsK"
                     /locus_tag="ACHJ8C_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO5550620.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHG
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPTQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPTRPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNTEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQPQYQQPQQPVAPQPHDTLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPV
                     DTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLA
                     RSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDI
                     AGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELS
                     VYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRM
                     MRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKAR
                     AAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDM
                     LYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGA
                     EELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNR
                     EVLAPPPFD"
     gene            43557..44168
                     /gene="lolA"
                     /locus_tag="ACHJ8C_000715"
     CDS             43557..44168
                     /gene="lolA"
                     /locus_tag="ACHJ8C_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO5550621.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            44179..45522
                     /gene="rarA"
                     /locus_tag="ACHJ8C_000716"
                     /gene_synonym="mgsA"
     CDS             44179..45522
                     /gene="rarA"
                     /locus_tag="ACHJ8C_000716"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO5550622.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAVGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            45613..46905
                     /gene="serS"
                     /locus_tag="ACHJ8C_000717"
     CDS             45613..46905
                     /gene="serS"
                     /locus_tag="ACHJ8C_000717"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO5550623.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            47144..49588
                     /gene="dmsA"
                     /locus_tag="ACHJ8C_000718"
     CDS             47144..49588
                     /gene="dmsA"
                     /locus_tag="ACHJ8C_000718"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO5550624.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATKMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARKKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQEFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGISNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            49599..50216
                     /gene="dmsB"
                     /locus_tag="ACHJ8C_000719"
     CDS             49599..50216
                     /gene="dmsB"
                     /locus_tag="ACHJ8C_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO5550625.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNATKGHMTKCDGCYDRVSEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            50218..51081
                     /gene="dmsC"
                     /locus_tag="ACHJ8C_000720"
     CDS             50218..51081
                     /gene="dmsC"
                     /locus_tag="ACHJ8C_000720"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO5550626.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAESQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(51117..51743)
                     /gene="ycaC"
                     /locus_tag="ACHJ8C_000721"
     CDS             complement(51117..51743)
                     /gene="ycaC"
                     /locus_tag="ACHJ8C_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO5550627.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            52057..53205
                     /gene="ycaD"
                     /locus_tag="ACHJ8C_000722"
     CDS             52057..53205
                     /gene="ycaD"
                     /locus_tag="ACHJ8C_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5550628.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGIGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQTENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            complement(53302..54042)
                     /gene="pflA"
                     /locus_tag="ACHJ8C_000723"
     CDS             complement(53302..54042)
                     /gene="pflA"
                     /locus_tag="ACHJ8C_000723"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO5550629.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(54234..56516)
                     /gene="pflB"
                     /locus_tag="ACHJ8C_000724"
     CDS             complement(54234..56516)
                     /gene="pflB"
                     /locus_tag="ACHJ8C_000724"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO5550630.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(56571..57428)
                     /gene="focA"
                     /locus_tag="ACHJ8C_000725"
     CDS             complement(56571..57428)
                     /gene="focA"
                     /locus_tag="ACHJ8C_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO5550631.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLIGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT        14005 a        14400 c        15321 g        13788 t
ORIGIN      
        1 atttgttaaa taccgttttt aaatccgagc tatagtctca aaccctggct aaagttattc
       61 ttgcgatgct tttatatagt gagcagtgca ggccgggaga gagttctctt ttcttacacc
      121 gcgccgataa aaatatgcac gtttattgca tatctttcag tgtgacaact tttgttcgtt
      181 tgttaacgaa ttttcagaag gaaagagata tgaccgcctt aaataaaaaa tggctatcgg
      241 gtctggttgc gggggctctg atggccgtct ctgtcagcac gctcgcggct gaacaaaaaa
      301 cactccacat ttataactgg tctgattata tcgccccgga cacggtggcc aactttgaaa
      361 aagaaaccgg tattaaagtc gtctacgatg ttttcgattc taacgaagta ctggaaggca
      421 aattaatggc ggggagcacc ggctttgatc tggtggtgcc gtcagcaaac tttcttgagc
      481 gccagctgac agcgggagtt ttccagccgc tggataaaag caaattgccg gagtggaaga
      541 atctcgatcc ggagctgctg aagttggtcg ccaaacacga tcccgacaat aaatttgcta
      601 tgccctatat gtgggcgacg accgggattg gctataacgt cgataaagtt aaagcggtgc
      661 tgggcgaaaa cgcgcccgtc gatagctggg acttgatcct caaacctgaa aatctggaaa
      721 aactgaaaag ctgcggtgtc tctttcctgg atgcgccaga agaagtgttt gctaccgtgc
      781 tgaattatct cggcaaagat cctaacagca ctaaagcgga tgattacacc ggaccggcta
      841 ccgatctgct gttaaagctg cgcccgaaca ttcgttattt ccattcatcc caatacatca
      901 atgatctggc gaatggtgac atctgtgtcg ctattggctg ggcgggcgat gtctggcagg
      961 cgtcaaaccg cgcgaaggaa gcgaagaatg gcgtgaatgt ctcgttctcg attccaaaag
     1021 aaggggcgat ggcgttcttt gatgtattcg ccatgcctgc ggatgccaaa aacaaagacg
     1081 aagcctatca gttcctgaat tacctgctgc gtcctgatgt ggtggcacat atctctgacc
     1141 atgtgttcta tgctaacgcc aataaagcag ccacgccgct ggtgagtgcg gaagtccgtg
     1201 ataacccggg tatttatccg cctgcggatg ttcgtgcgaa gctgttcact ctgaaagtgc
     1261 aggacccgaa aatcgaccgt gtgcgcaccc gcgcgtggac caaagtgaag agcggaaaat
     1321 aatccgcagc cgtagatgcc ggacgggcgc accacacccg ccggcaattc gcaccatcat
     1381 ggtgcgcttg cacacattca atgccggaga gcagccgtga atgacgctat ccctcgcccg
     1441 caggcgaaaa cccgtaaggc gctgacgccg ctattagaaa tccgtaacct gaccaaatcc
     1501 tacgatggtc aacatgcggt ggatgatgtc agcctgacca tctacaaagg tgaaatcttc
     1561 gcgctgctgg gcgcatccgg ctgtggcaag tccacgctgc tgcgtatgct ggcaggtttt
     1621 gaacaacctt ctgccgggca gattatgctc gatggcgtcg atttgtcaca ggtgccgcct
     1681 tacctgcgcc ccatcaatat gatgtttcag tcttacgcgc tgtttccgca tatgaccgtg
     1741 gaacagaaca tcgcttttgg cctgaaacag gacaaactac cgaaagcgga aattgccagc
     1801 cgggtcaatg agatgcttgg gctggtgcat atgcaggagt tcgccaaacg caaaccgcat
     1861 cagctttccg gtggtcagcg tcagcgtgtg gccctggcca gaagccttgc caagcgcccg
     1921 aaactattac tgctcgatga gccgatgggc gcgctggata aaaagctgcg tgaccggatg
     1981 cagcttgaag tggtggatat tctggagcgc gtcggtgtga cctgcgtaat ggttactcac
     2041 gatcaggaag aggcgatgac tatggcgggg cgcatcgcca ttatgaatcg tgggaaattt
     2101 gtccagattg gcgagccgga agagatctac gagcatccga ccactcgcta cagcgccgaa
     2161 tttattggct cggttaatgt ctttgaaggt gtactcaaag agcgtcagga agatggtctg
     2221 gtgctttatt cgccggggct ggtgcatcca ctgaaagtcg atgtggatgc ttcggttgtc
     2281 gataacgtgc cggtgcatgt ggcgcttcgc ccggaaaaaa tcatgctttg cgaggaaccg
     2341 cccgccaatg gttgtaactt cgcggtgggg gaggtgatac acattgccta tctcggcgat
     2401 ctttcggtgt atcacgttcg tctgaaaagt gggcagatga ttagcgccca gctacaaaac
     2461 gcccatcgcc atcgtaaagg gttaccgacc tggggcgacg aagtacgttt gtgctgggaa
     2521 gtggacagct gtgtggtgct gacggtttaa ggagcaaaga tgagtacact tgaacctgct
     2581 gcccagccaa aaccctttaa gctgtggttg tcgcagctgc aaatgaaaca tgggcgcaaa
     2641 ctggttattg cgttgccata tatctggttg atcttgctgt ttctgctgcc atttctgatc
     2701 gtctttaaaa taagccttgc ggagatggcg cgcgctattc caccttatac cgaattgatg
     2761 gagtgggctg acgggcaact ttctatcact cttaatctcg gtaattttct gcaactgacc
     2821 gacgatccgc tctattttga tgcttatctc cagtcgttac aggtggcggc gatttcgact
     2881 atttgctgtt tactgatcgg ctatccgctg gcgtgggcgg tggcgcacag taagccttcg
     2941 acccgtaata tcttattact actggtgatc ctgccgtcgt ggacctcgtt tctgattcgt
     3001 gtctacgcct ggatgggaat attaaaaaac aacggtgtgc tgaataattt tctgctgtgg
     3061 ctggggatta tcgatcaacc gctgaccatt ctgcatacca actttgcggt ttatattggc
     3121 attgtttacg cctacgtgcc gtttatggtg ctgccgattt acaccgcgtt gattcgtatt
     3181 gattattcgc tggtagaagc ggcactggat ctcggtgcac gaccgctgaa aacgttcttt
     3241 actgtgatcg tgccgctgac taaaggtggg attattgccg gatcgatgct ggtgtttatc
     3301 ccggctgtgg gcgagtttgt gatcccggaa ctgctcggtg gcccggacag catcatgatc
     3361 gggcgcgtgc tgtggcagga gttctttaac aaccgcgact ggccggtggc ctcagcggta
     3421 gcgatcatca tgttgctgct gctaattatg ccgataatgt ggttccacaa acatcagcaa
     3481 aaaagcgagg gagaacacgg atgaataatt taccggtggt tcgttcgccc tggcggattg
     3541 tgattttgct gttgggcttc acctttctct acgcgccaat gctgatgctt gttatctatt
     3601 cgtttaacag ctcgaagtta gtgacggtgt gggccggctg gtcaacgcgc tggtatggcg
     3661 aattattgcg cgatgacgca atgatgagtg cggttggttt aagtctgata attgcggcct
     3721 gtgcggcgac ggcggcggcg atcctcggga ctattgcggc ggtcgtgctg gtgcgctttg
     3781 gcaggtttcg cggatcaaat ggttttgcct tcatgatcac cgcaccgctg gtaatgccag
     3841 atgtcatcac gggtttgtcg ctgttgttgt tattcgtcgc gcttgctcat gctattggct
     3901 ggcctgcgga ccgcggtatg ctcaccatct ggctggcgca tgtcactttc tgtacggctt
     3961 atgtggcagt cgttatttcg tcgcgtctgc gggaactgga tcgctcgata gaagaagccg
     4021 cgatggatct cggtgcgacg ccgctgaaag tgttttttgt cattacgcta ccgatgatca
     4081 tgcccgcgat catttctggc tggttactgg cttttacttt gtcgcttgat gatctggtga
     4141 tcgccagctt tgtttccggg ccaggagcca ccactttacc gatgctggtc ttttccagcg
     4201 tgcggatggg ggtgaatccg gaaatcaacg ccctggcaac attgatcctc ggcgcggtcg
     4261 gaattgtcgg atttatcgcc tggtatctga tggcgcgcgc agaaaaacag cggatacgcg
     4321 acatccagcg tgcaagacgt ggctgaagac actaaaattt gccaacctgg ctacataatg
     4381 ccgcgcatgt cgcggcattg ttttcatgga agacgaaacg ttgggatttt ttaagaaaac
     4441 atcttcatct catgctcgcc tgaatgtgcc tgcgctggtg caggtggcgg cgctcgccat
     4501 tattatgatc cgtggcctcg acgtgctgat gattttcaat acgctgggcg tgcgcggtat
     4561 tggcgagttc attcatcgca gcgtacaaac ctggagttta acgctggtct ttttgagcag
     4621 tctggtgctg gttttcattg agatctggtg tgcgttttca ctggtgaaag ggcgtcgctg
     4681 ggcgcgctgg ctgtatctgt tgacacaaat cacagccgca agttacttgt gggcggcctc
     4741 gctggggtat ggttatccgg agctgttcag cattcccggt gaatcaaaac gtgaaatctt
     4801 ccatagcctg atgctgcaga agctgccgga tatgctcatc ctgatgctgc tgttcgttcc
     4861 ctcgaccagt cggcggttct tccagttgca ataatgtgta taatcgtcgc ccctgatgat
     4921 gtgaaggtca atgtatgcag tgcgcacttt acgacgcggg tcgctgtcgt tcctgtcagt
     4981 ggataacgca gccgattcca gagcaactct ccgctaaaac cgtcgatctt aaaaatctgc
     5041 tcgccgattt tccggttgag gaatggtgtg cgccggtgtc aggcccggaa caagggtttc
     5101 gtaataaagc caaaatggtg gtgagtggta gcgttgaaaa accactgctc ggtatgctgc
     5161 atcgggatgg tacaccggaa gacctttgtg actgcccgct ttatccagcc tcatttgcgc
     5221 ccgtttttgc ggcgctaaaa ccgtttatcg cccgtgcggg tttaacgcct tacaacgtgg
     5281 cgcgtaaacg tggtgaactg aaatacattc tgctgactga aagccagagc gatggcggca
     5341 tgatgctgcg ttttgtactg cgttctgata ccaaactggc gcaactgcgt aaggcgctgc
     5401 cgtggttaca ggaacaatta ccgcagctga aagttattac cgtcaatatt cagccggtac
     5461 atatggcgat tatggaaggg gagacggaga tctacctgac cgaacaacag gcactggcag
     5521 agcgttttaa tgacgtaccg ctgtggatcc gtccgcaaag tttcttccag actaatccgg
     5581 cggtcgccag ccagctgtac gccaccgcgc gcgactgggt acgacagctg ccggttaaac
     5641 atatgtggga tctgttctgc ggtgtgggtg gctttggttt acactgcgcg acgcctgaca
     5701 tgcagttaac cgggatcgaa attgcaccag aggccattgc ctgtgcgaag cagtcagccg
     5761 ctgaactggg cttaacgcgt ttgcaatttc aggcgctgga ctctacgcag tttgccaccg
     5821 ctcaggggga agtgccggag ctggtgctgg ttaacccgcc gcgccgcggc atcggtaaac
     5881 cgctgtgtga ttatctctca acgatggcac cgcgttttat tatctactcc agctgtaacg
     5941 cccaaactat ggcgaaagat atccgcgaac tgcctggtta tcgcatcgaa cgggtacagc
     6001 tttttgatat gttcccgcac accgcgcact atgaagtgct gacgctgctg gtgaagcaat
     6061 aaaaaagccg caggtgcggc tttctgaatc ttactgtggg aaccactggt cattgatttt
     6121 ctgatatgta ccgtcagctt taattgctgc cagcgcgtta ttcagttttt ccagcagagc
     6181 tttgttatcc ggacgtacag cgatacccag gccggtgccg aagtattgcg gatcggtcac
     6241 tttttcagta gcaacgccca gttgcggatt ggttttcagc cattcgttta ccactgcggt
     6301 gtcaccaaat acgccatcaa tacggccatt tttcagatcg ataaaggcat tctgataact
     6361 gtcataggag acagttttta cttccgggtg ctgatcctga atatatttct ggtgcgtggt
     6421 gccgttttcc atcccaatac gtttgccttt cagatcggca aacgttttgt aggtatcttt
     6481 tttggcaatc acgacggctg agttttcgta gtagggcgtg gtaaacgata cctgtttgct
     6541 acgctccggc gtgatatcca ttccggagat tacggcgtca tattttctga atttcagtga
     6601 cgggatcagg ctgtcgaacg cgtgattagt aaaagtacat tctgcctgca tttgtttgca
     6661 caaggctttt gccaggtcga tatcaaagcc gacaatctca ttattagcac ctatagattc
     6721 aaagggtgga taggtggctg aaacgccaaa attgattttc tctgcggcag aagcaccgaa
     6781 agtaaaagaa gcaagtaaag cggcaagaac taactttttc atgatggaac tcccgtctgt
     6841 caatcttatg atttttggcc gtgtctgcgg catgggataa caatgccatc aagtgaattt
     6901 atatgcaata aatatgagta aataatttat atgaaataaa aaaagacgga cagcttagtg
     6961 ggttgtccgt cttcataata agaatttatg cactatgtag gccggataag gcgtccacgc
     7021 cgcatccggc atcagcatgc agctacatta attccgccgt tcgaatgcca gtgctttacg
     7081 ctcgatcaga cgcatcatca gcgtcagcag gccgttgacg accaggtaaa taatccccgc
     7141 cgcgccaaat accattacat cgtaggtgcg tccgtacaac aactggctgt atcccatcac
     7201 ttccatcagc gtaatggtgt aagccagaga ggtacttttg aacaccagca ccacttcgtt
     7261 ggaataagaa gagagcgagc gtttaaacgc gtacggcagc aggatcgcca gcgtatcttt
     7321 tttgctcatt cccagggcgc tacaggactg ccattgacct tccgggatcg cgcgaatcgc
     7381 accgtaaaac agctgcgtgg tgtacgccgc actgttcagc gacaacgcaa tcagtgcaca
     7441 taaccacggt tctgacaaca aatgccacag tgccggatac tcctgcaacg ttgggaactg
     7501 gcccggtccg taataaatca ggaagatctg caccagcagc ggcgtaccgg taaacagcgt
     7561 gatatagccc cgcaccagcc acaccagcac cggcgttttc agcgtcagga tgatggtaaa
     7621 aatcaatgcc agaatcagtg ccacaatcag cgaggcaacg gttagcgtca ggctggtgtg
     7681 cagccctttc atcagttcgg gtaaatactc aaacattagc tgggcctccg ctcaaaacgt
     7741 gtcgcgcgca ggtcaatgcg tttgagaatg tactgactga gcagggtgat caccaggtaa
     7801 atcaccgccg ccacaatgta ccaggtaaac ggttcctgtg tacgggtagc gatgcttttg
     7861 gtttgtagca ttaaatcatt cacactaatc aaactgacca gcgcggtatc tttcagcagc
     7921 accagccact ggttaccgag acccggcagc gcatgacgcc acatctgcgg catcaccaga
     7981 cggaaaaaga tagccgattt cgacagcccc agcgcctgac cggattccca ctgacccacc
     8041 ggcaccgctt ttaatgcacc acgcagcgtt tgcgaggcat aggcggcata cagcagtgac
     8101 agtgcgatga caccacaaag gaacgggcta acgtcgaagt tctcaatgtc catctgcacc
     8161 gggatctgca cgaacccaag attgatagtg aagccatccg aaagcgtcag cagcagctgc
     8221 gaggagccaa aatagataaa cagcaccacc agaatttctg gcaggccacg cagaatggtt
     8281 accagcgctg aacctgccca cgcgacagga cgccatttcg cagactccca taccgcaaag
     8341 aacatcgcca gcgccagccc gacaatcaat gcacaaacgg caaggccgac ggtcatcccg
     8401 gcggcgcttg ctaaaggaaa aaattcattc atcaggaatt acttctggaa ccatttgttg
     8461 tagatggttt cgtaagtgcc atctttcttc actttttcca gcgcagtgtt gagtttctgc
     8521 tgcagttcag tgttgccctg gcgtactgcg atgccgaggc cagtaccgaa gtaatcttta
     8581 tcggtcactt tatcgcccac tgccgccagt ttcgggttat ctttcagcca ttcggttacc
     8641 actgcggtgt caccaaatac gctgtcgata cgaccgtttt gcagatccag ttttgcgttc
     8701 tggtaactgt catatggcac ggtagtgatt tccggatgct tatccataat gaacttctgg
     8761 tgtgtcgtcc cgttctgaac gccgactttt ttgcctttca gctgatcaac actggtgtat
     8821 ttgccttgct gacccacaaa cagggcagag ttgtcatagt acggggtggt aaacagcacc
     8881 tgcttttcac gctccggagt gatatccatg cccgccatca ctgcttctac gcgacggaat
     8941 ttcaggcttg ggatcaggct gtcaaacgcc tggttagaga aagtgcaggt tgcatcaatc
     9001 tctttacaca atgcctgcgc caggtcgaca tcaaaaccaa cgatctggtt gtttgcatca
     9061 atcgattcaa acggaggata ggaggcttcg gtggcaaaac gaatggtttc ggctgctgtg
     9121 gcggaaagac taaaacctgc aattaacgcg gcaatcagaa cttttttcat tgttgttatc
     9181 ccgaatctta gtgagagaga tagtttttaa atgcttcggt ttgcggctcg gtaaagcagc
     9241 tcgcgtcgcc ttgttctacg atatgaccgt tttccatata caccactcgg ctggcggttt
     9301 tacgcgccac ttcaacttcg tgggtgacga tcacctgggt aatattcgtt tctgccagct
     9361 cacgaatgat gctgacgatt tgtgccgtaa tttcggggtc cagtgcggcg gtcggttcat
     9421 cgaacagcag tacctgcggt tccatcatca acgcacgggc aatagcaaca cgctgctgct
     9481 gacctccaga aagatgcagc gggtaacgat cgctataagg tttgagacgc agacgttcca
     9541 gcagtttttc tgcacgggcc agcgcctgat ctttactcaa ccccagtaca cgacagggcg
     9601 cttcaatcag gttttgctgc acggtcagat gcggccacag gttgtattgc tgaaacacca
     9661 tgccaacgtt acgacgcaaa tcgcgaatcg ctttgtcaga gggggttttg gtgaaatcga
     9721 aatggttgcc tgcaatgttg agcgtaccgg agcgcggcat ctcaagcaga ttgagtacac
     9781 gcagcagcga gcttttaccc gcgccgctgg ggccaagtaa caccagcgtt tcgccctgtg
     9841 ggcaatccag cgtgatatcg aacagcgcct gatgcgcgcc gtagaagcaa ttaatgccgt
     9901 ttaattgaat actcattgac actcgtatac tggcagtctg atagctattg aggtcgaaga
     9961 tagtaccttt gacagaataa ttatgcaata tttctgcttt aaaagttaaa agcaaagcgc
    10021 attattcaat aaacatagca caaaataacg ggggcggtgg tcggcgagca taaatgtcgg
    10081 cattcctcac gaaatgccgt acaatttacg gggtttattg gttgatcaag acgttagcga
    10141 ttctcgatgg actgacggag cgtacccgct gtggcatgaa cgctaccgcc taagtagcgc
    10201 acatcgtcga tgacccaaca ctggccttcc tgaatcatta acacttcatc ctgccagccc
    10261 tggtcaccct gcttgagatc cacgcgcaat ggaatgttac gggcatcacg attagggata
    10321 gtagatgcac tggcaacgtg ggcgctgtct ggcaaggtgg ttcgactgga gaacggatcg
    10381 ctgcttaaca attcacgatg actattatcg cggctggcat cgctaagtag ggttgccagt
    10441 ttgtcactca aataggggcg tagagcggta atgtcattgc cgcgatgttg aatgcggtag
    10501 tcatagaact gttgagccac gctatcaggc cccccctgga cacaggaacc ggtgcggggg
    10561 ccgttatctt tataagctgg cgtgacggtg gtacaggcac tgagtagcag tgcgcaaggg
    10621 ataagcatgg tcaatttgct atagcgcatg atgatttcct tataagcgaa cacattgaaa
    10681 gcgttctacg ataataatga tatcctttca ataatagcgt atcagtccga taatgctttg
    10741 aagatcgaag gcttagcaaa caaggagatc gatcatgcaa ttttctacaa ccccaactct
    10801 ggaaggccag accatcgttg aatattgcgg tgtggtgact ggcgaagcga ttttaggcgc
    10861 gaatattttc cgtgatttct ttgccggtat ccgcgatatc gttggcggac gctccggtgc
    10921 ctacgaaaaa gaactgcgta aagcgcggga gattgccttt gaggagctcg gctcccaggc
    10981 gcgagcgctg ggggctgatg ccgtcgtcgg tattgatatc gactacgaaa cggtcgggca
    11041 aaacggcagt atgctgatgg ttagcgtcag cggtacggcg gtgaaaacgc gtcgatgaga
    11101 agagtcttct ggctggtcgc tgccgctctg ttactggcag ggtgcacagg cgaaaaaggc
    11161 attgtcgaga aagagggata tcagcttgat acccgacgcc aggcgcaggc ggcgtatccg
    11221 cgcattaaag tgctggtgat ccactacacc gcagatgatt ttgatagctc gctggcgaca
    11281 ctgaccgata agcaggtcag ctcgcattat ctggtccctg cggtgccacc gcgatacaac
    11341 ggtaaaccgc gcatctggca actggtgccg gaacaagaac tggcctggca tgcggggatt
    11401 agcgcctggc gcggggcaac gcgccttaac gacacctcta ttggcattga gctggaaaac
    11461 cgtggctggc aaaaatcggc gggagtgaaa tattttgccc cgtttgaacc ggcacagatt
    11521 caggcgctta ttccgctggc gaaagatatt attgcccgtt attacatcaa gccggaaaac
    11581 gtagtagcac atgcggatat tgcgccgcag cgcaaagacg atccggggcc attatttccc
    11641 tggaagcaac tggcgcagca ggggattggt gcctggccgg atgcgcagcg ggtgaacttt
    11701 taccttgccg ggcgcgcgcc gcacacaccg gtagagaccg cgtcattgct ggagcttttg
    11761 gcgcgttatg gttatgacgt aaagcctgat atgactccgc gcgagcagcg gcgcgtgatt
    11821 atggcgttcc agatgcattt ccgcccgacg ttatacaacg gcgaagcgga tgcagaaact
    11881 caggcgattg ccgaagcgtt gctggagaaa tacgggcagg attagcgcgg cagttttccg
    11941 tggtcgcgta gccaggcggc agttttctcg ataccttcat ccagggtgag gactggttga
    12001 taacctaact cttcctgcgc acgcgtaata tccagcgtaa agtcaaaatt caacttggag
    12061 acgccgtagt gggtcagcgg cggctctttt gctgacttgc ggcctaaacg ctccatgctg
    12121 cgggcgatca tatccagcat cgggtaaggg acggaacgaa tacgacagtc aatattcaac
    12181 tcgtcgatca gcttctgcac gatgctgcgc agtgtgcgat gctcgccgtt ggtgatgttg
    12241 tacacacgcc cggaaggtag cttatcgcag gcttcctggc ttgccagcca catggcgtgc
    12301 acggcatttt catagtaggt catatcgacc agcgcactgc cgccgtgcgg taacagaatg
    12361 ctgccgtagt ggtgcatcat atgcgccaga cgggggataa agactttatc gtgcggtccg
    12421 aacagacttt gtgggcgcag aatagtaaag cgcgtttgtg gattcgcctg cgaaagcata
    12481 ttgatcacct cttcgctggc tgctttgctg cgggcaaact cgttggcgaa gcggtgaggg
    12541 cgaaaatctt ctttaatatc gcgatggtgg tgataatcga agtacaggga gggggaagag
    12601 atatgaataa agttacgcac accccaggcg acagcccatt cacccaggcg gcgagtggcg
    12661 cgaacgttag ccagatcgaa agcctgttgt gtcccccagg gcgaggtaaa gctggagcag
    12721 tgccacagcg tatcaatgcc cgcgagcatc actttagctt gtgatgaaac cagctcggtc
    12781 agatccgccg gaacaaactc tgcgcccatt ttttccagca atttacccat tgcctcgttg
    12841 cgaccggtcg ctcgcacgct gatgcctttc tggcataaaa actctaccgc gtttcgacct
    12901 aagccgctgg tggcgccggt aaccagtacc ttcatatcaa tccactgttg ttgagaaaat
    12961 aacgtgcgca ttcttccgtg atttccccca tgatgcaatg ggaaacatga aagaataacg
    13021 caggttttgt cgattaatct gtgctttgtt ctgccagtct ggcgattcgt tttgccattc
    13081 cgcgaaaaat aaacagatgc gcggggatca tcaataacca gtaaaacaac cctggcatac
    13141 cgtgcggatg ccagaatgcg cgaacatcga tagtacgata gtcaccttta tcttccaggg
    13201 taaaacacag tcgtcccagc cccggcgctt tcatgccaaa taacagcgta agttgttttt
    13261 ccggttcaac gacaatgact ttccagctat ccaccgcatc gccagtttgc aaatattcgc
    13321 gctccgggcg gccttttgcc agtttatgac cgatcgcaag gtccatcagc gctcgagtct
    13381 gccacaaaat attgccaaag aaataacgct ctttaccgcc gatttgattg accacccgcc
    13441 atagcgcctg aagtctggca gacgttttaa cggtaaaccc cgcctgtttg gcaaaataac
    13501 cgtattctgg tcgccagcga gcaaaagcct gcgcgtcata tccccagtcg ctggagttga
    13561 ccagtttttc ttcctctttc aacgtgctac gtaccgcgtc atcgaaagca atcagcggtt
    13621 gtggaatgag tgcacgtaac gcggtgtcat ctgccagcag atcgtgtttc agcccctgaa
    13681 tcaacgccct ggcggtggtg ggtggtacgg aagtaatcac attaagaaac cacaccgaaa
    13741 tccagcgggt gggaaagggg atggggagca accagcggcg cttaccgctc accgccataa
    13801 aatgttcaaa ctgttgctga taactgagca cttctggtcc ggcggcttcg aagatgcgat
    13861 gttcgctggc tggatgatct aacagcgcca ccagatagtg cagcaagttt tccagcgcga
    13921 tgggcgtggt gcgtgaacgg acccagcgtg gcggcgttaa cactggcagg ttgtagacca
    13981 tatcgcgcat gacctcgaac gccgctgaac ctgcaccaac gattattccg gcacgcaatt
    14041 cggtcaccgg gacacccgct tcacgaagaa tgtccgccgt agcctgacgg gcgcgcagat
    14101 ggtccgactg ctcatgtggc ggggcctgca acgaactgag aaagattaat tgcttaactg
    14161 ggacttcacg tagcgcatcg cggacgttga gagccacctg gcgctcctga gcgataaaat
    14221 cgccgccttc gcccatgctg tgcaccagaa aatagaccgt atcaatatcc tgcaacaggg
    14281 ccggaaggtt atccggccag ctgagatcga ctttatggca actgacattt gccagttgca
    14341 gctttgcaag cctgtcgaca tgacgtgccg ccgccaggag ctgatgccct tgctggctga
    14401 gtgtgcgcac cagatgctga ccaatgtagc cactggcacc gagaactaaa atgcgttgcg
    14461 gcacgtctcc ctccttaacg cgccaggaat gcgcgccagt gggcggcgac ttccgccagt
    14521 tgtgcacgcg agacgtcaag atgggtcacc aggcggacaa tcggcgaggc gttaatcagc
    14581 acgtttctcg ctttcatgta ttcgcctaac gcggcagcat tttcttcccc gacgcgaaca
    14641 aacagcatat tggtgtcctg acgcatcaca tccgcgcctg cttcacgcaa ctgctccgcc
    14701 atccaggtgg cattgtcgtg gtcttcctgc aaacgtgcga cgttattttt cagcgcatac
    14761 atcccggcgg cagccagaat gccggactgg cgcatcccgc cacctgccat tttccgccag
    14821 cgaatggcac gtttaatgta atcacgatta ccgacgagta atgaaccgac tggcgtccca
    14881 agacctttcg acaggcaaat ggtgaacgag tcacaatatt gcgtgatctc tttcagttcg
    14941 cagccgtaag ccaccacggc attaaagatg cgcgcaccgt caacatgcag cgccagattg
    15001 cgcttgcggg taaattccca tgcctctttt aagtattcac gcggcagcac tttgccgttg
    15061 tgggtgtttt ccagactgag taatttggtg cgggcgaaat ggatatcgtc gggtttgatt
    15121 ttcatcgcca ctttatccag cggtagcgtg ccgtcggcag ctgcgtcgat gggttgcggc
    15181 tgaatactgc ccagcactgc tgcgccaccg gcttcaaaca gatagttatg cgcggcctga
    15241 ccgacgatat actcttcgcc gcgttcgcag tgactgagca gagcgaccag gttggcctga
    15301 gtgccggtcg gcagaaaaat ggcggcttct ttaccggaaa gctctgctgc atagtcctgc
    15361 agagcattaa cggtagggtc gtctccgtaa acgtcgtccc caaccggggc ggccatcatc
    15421 gcttcgagca tggcgcggct cggtcgggta acggtatcac tgcgtaaatc aatcatggca
    15481 tgtccttatt atgacgggaa atgccaccct ttttacctta gccagttcgt tttcgccagt
    15541 tcgatcactt catcaccgcg tccgctgatg attgcgcgca gcatatacag gctgaaacct
    15601 ttggcctgtt cgagtttgat ctgcggtgga atggctaatt cttctttggc gacaaccaca
    15661 tccaccaaca ccggaccgtc gatggagaag gcgcgttgca gagcttcatc gatttcagac
    15721 gctttttcta cacgaatacc cgtaatgccg caggcttcgg caattcgggc aaagttggtg
    15781 tcgtgtagtt cggtgccatc agtcaaatag ccaccagctt tcatctccat cgccacaaag
    15841 cccagcacgc tgttattaaa gacgataatt ttcactggca gtttcatctg cactaccgag
    15901 aggaaatcgc ccatcaacat gctaaaaccg ccatcgccgc acatggcgac cacctggcgt
    15961 tccggctcgg ttgcctgcgc gcccagcgcc tgcggcatgg cgttagccat cgaaccatgg
    16021 ttaaacgaac ctaacaggcg acgcttgccg ttcattttta gataacgtgc cgcccacacc
    16081 gttggcgtac caacgtcaca ggtgaaaata gcgtcatcgg cggcaaaatg actaatttgc
    16141 tgcgccagat attgcgggtg aatggctttc tcactcggtt tagctaaatc gtccagccct
    16201 ttgcgggcgt cgcggtaatc ttccagcgcc ttatccagaa acttgcgttc ggttttttct
    16261 tccaccagtg gcagcaatgc gcgcagggtt gatttgatat cgccgaccag cgccatatct
    16321 accttgctat gcgcgccgat gctggctggg ttgatatcaa tctgaataat tttggcatcg
    16381 gtcgggtaga acacacggta gggaaactga gtaccgagca gcactaacgt atcggcgttc
    16441 atcatggtat ggaaacctga cgaaaagccg attaatcccg tcattccaac atcatatgga
    16501 ttatcgtatt cgacatgttc tttaccgcgc agggcatgga ctataggcgc tttaattttc
    16561 ccggcaaact caactaactc tttatgcgcc cccgcacagc cgctgccaca catcagggcg
    16621 atattgctgg aataacgcag cagttgcgcc agtttgcgta actcttcttc ttccggtgtt
    16681 actaccggct gtggcgcatg ataccagtgg gtagttgccc cttctggcgc aggttttaac
    16741 gccacgtcgc ccggtaacac aacaacggaa acgccacggt taagcaccgc tttgcgcata
    16801 gcaattgcca gcacttgtgg gatctgctct gggctggaaa ccagctcgca atagtgacta
    16861 cattcgcgga ataactcttg tgggtgggtt tcctggaaat agccgctgcc aatttcgctg
    16921 gagggaatat gagcggcaat cgccagtacc ggaacgtgat tgcggtggca atcgaacagg
    16981 ccgttgatta agtgcaggtt gccgggaccg cacgatccgg cacagaccgc cagttctccg
    17041 ctaagttgtg cttcagcgcc agcggcaaac gccgccactt cttcatgccg ggtggacatc
    17101 cactcgatgg tgcccatgcg attaagactg tcactaagac cgttcagaga gtcgcccgtg
    17161 actccccaga tgcgtttcac gcctgccgat tcgagtgttt tggcgatata agctgcaacc
    17221 gtttgtttca tggttctcca tctcctgaat gtgataacgg taacaagttt agttcatctg
    17281 acggaggggg aagggatggg agaaaaagga ggcactaacg gttaaatagc ccgatgaaag
    17341 gaatatcatc gggcatcagg cgattatgcg agaaccaaat ccccctgcgg atggcaggag
    17401 caggccagta cgtaaccttc agcgatttcg gcgtcggtca gcgtcattgt gctgctcacc
    17461 gtatattcac cggaaatcac tttcgtctta cagcagccgc aaacacccgc acggcaggcg
    17521 gcgacaaccg gaacgttatt gctttccagc gcctccagta gcgtggtgcc aaccggagcg
    17581 taaaattctc gtgccggttg cagtttggtg aatttcagac cgctggtcgc cgcttccgct
    17641 actggggtga agaatttctc tttaaagaaa cgcgtcacgc cgagcgtttt cacttcctgc
    17701 tctacccaat ccatatatgg agcagggcca caggtcatca cggtacgtga ggctaaatca
    17761 gggacacctg ccagcagttc gcgagtgaga cgaccagcga taaagccttc ggtaacgtta
    17821 ttttccgcca ccagcgttac cggatagtta cgccactcat cggcgaaaat cacatcctgc
    17881 ggcgtacgca cgttgtagat cacctgcaca tcggcctgtg gacggttctt cgccagccag
    17941 cgacgcatcg acataatcgg cgtaacgccg cagcctgccg ccagcaacag gaatttatct
    18001 tctgctttat cgtcgcaggt aaattccccc atcgcgtccg aaagccagag ataatcaccg
    18061 cgttttacat cgcgcgtcag ccaccgggag ccgacaccgt catcaatccg ccgcacggtc
    18121 agggtgatgt attcactcac gcctggcgtg gaggaaatgg tgtaagcacg cagcgtttcc
    18181 gctgagttac gcacgctgac cagcgcatat tgcccggcgc gatacgggta gtaatcgtgg
    18241 caaatcaggg aaatcgtcca cacatccggc gtttcttgcg taatgtgatg aacctgcatc
    18301 cgccacgggc attgattcgt tggcatcgtc atcgacaaac tccttacgcg ctcaacagtt
    18361 gcttcatgtc ttcttcaaca gtggtgatag aacgcaggcc gaatttctcg ttcagcaccg
    18421 ccagcaggtc tggtgtcagg aaaccaggtg cagtcgggcc ggtgacgata tttttcacac
    18481 ccagagaaag cagcgtcagc agaatgacaa tcgctttctg ttcaaaccag gagagcacca
    18541 gcgacagcgg cagatcgttg actccgcagc ccagtttctc tgccagcgtt accgccagaa
    18601 taattgctga gtaagcatcg ttacattgac cagcatctac cagacgcggc agaccttcga
    18661 tatcgccaaa ctcaagttta ttaaagcgat atttaccgca agcgagggtc aggatcaggc
    18721 agtcatccgg cacgctggtg gcgaaatcgg tgaagtagtg acgctcgccg cgtgcgccgt
    18781 cacagccgcc aagcaggaag atatgacgca gtttttcacg gctcaccaga tcaatcagcg
    18841 tatcggctgc gccaagcagt gtctggcgac caaagcccac ggtgatcagg tgcggaattt
    18901 cgctgtacgg gaagcccgcc atctgttgcg cctgggcaat gactgcagag aaatcttcgc
    18961 catccagatg acgcacaccc ggccagccaa cgatgctgcg ggtccagata cgatcgtcat
    19021 aagcgcctac ggttgggtcg atgatgcagt tcgaggtcat cacgatgggg cctgggaaac
    19081 gagcgaactc cacttgctga ttctgccagc cgctgccgta gttaccgacc agatgcttga
    19141 atttacgcag ctccgggtaa ccatgcgcag gcagcatttc gccgtgggtg tagacattaa
    19201 cgcccgtgcc ttcggtctgt tccagcaggt tgtagagatc tttgagatcg tgaccggaaa
    19261 tcagaatgca tttacccgcc gtcgctttga cattgacctg ggttggggtc gggtgaccgt
    19321 atttaccggt ttcgcctgca tccagaatgc tcatcacttt gaagttcatc tggccgattt
    19381 ccattgaaca ctcaagaagc gcgttcatat cggcaggcca ggtccccagc cacgccatga
    19441 ttttatggta ctgggcataa atatcgttgt cgtattgacc gagaacatgc gcgtgttcca
    19501 tataggccgc cgcacctttc aggccataca ggcacagtag acgcaggccg agaatgtttt
    19561 cgccaatcgc cgctttatct ttgttagggg taaattctgc tgcctgacgt tgcagctcgc
    19621 cgagatcgtc gctcaccagt tgcaggtcag ccatcgggtt atcgacgcgg gcgttggcat
    19681 ctacagccag gcattgtgct ttcagcgcct cgcgcagggc aatcgcttca cgagcgtagc
    19741 cgacaatacg cggagaatcg aagttaacgt tggtcagggt tgagaaaaag gcacgtggcg
    19801 caaagctgtc tacatcgtgg ttgatgatgc cgtattcacg tgctttcact gcccaggccg
    19861 aaagcccttg cagcgccgcg atgagtaaat cctgaaggtc agaggtttcc gccgttttac
    19921 cacacatccc ctgcgcgtat gagcagccgt ttcctgccgg agtacggata gtttgttcac
    19981 attgcacaca aaacatgatc acaccttttt aaagttatat ttaatataca tgtttaaggt
    20041 taagacgctt aacgcgggga taaaagagat ttttaatgca actttaaggg agattgattt
    20101 agcgcaattt tggcggcaga gatctaccgc cagagaggta ttatgcagag aaaaaggcga
    20161 tgagaatcgg cactaacaga ctaagaataa aaccgtgaac aatcgccgcc gggaccatat
    20221 ccagcccgcc tgtacgttga agaacgggca gggtgaaatc catcgacgtc gcaccgcata
    20281 agcccagtgc agtagagcgg ctacggcgaa tcagcccagg gatcaacata attgcaatca
    20341 gctcacgggc cagatcatta aaaaacgccg cgcttccgat taccggacca aaagattcgg
    20401 tcaataaaat accggaaaga gaataccagc cgaaaccgga ggccattgcc agcgcggtat
    20461 taatgggaag atcaagaata aaggcgttaa ttaaaccacc aattaatgaa ctggcaacca
    20521 ccaccacggc gacaatcatt ccccggcgat taaggacaat ctgctttaag gtcatgccat
    20581 tattgcgcaa ctgaataccg acgaggaaaa gcagcagaat taaggtgtat tcactggctt
    20641 cggtcgcgtg ttgtaagaaa gccagtccac tcaggccaat ggcaaaacca atcactacta
    20701 cgccgcacag ttttagcgac tccagcgcca tcgcaatacg cgacgggagt ttttcttgct
    20761 gatggtgatt gcgccatggc aggccgcgct ccagccacat cagggcggca atattacaca
    20821 gtaaaataac ggtaatactg acggcagaat aatgcagaat cgccaacagg ttactggcga
    20881 ggttatcgag aaacgccaga ctgataccca taaaaaagag aataaggtaa accatccagc
    20941 ttaatagctg attaataact cttaatgcag ctttttggcg aagcggaatg aggtaaccca
    21001 caatcagggg aaccagaatg attaacagcc cagaaaacat gaaaacccag tccttgcaaa
    21061 gatgaagtcg aaatgcgcga tgacacacta ctgaaagcgg aaggacgagt aaagttgcaa
    21121 ttaaaaggaa atgttatgca tcagaggggg atttttaatt ctatatgagt gtttagcatt
    21181 gttgcgctcc tttcatgtgc aatgccatat aaagccactg gcgcagggag cggccagttg
    21241 ccgccgctcc ctgccccccg gccttcgggg aacgcctcgg cgattttaac gccaccaaca
    21301 cccgtgcggt ttgtaacgtc gaagataaaa ccaccagggc agacaacatg ccaatgctaa
    21361 ttaatcgcgt ttttccagta gagtccggta aatcagacca ccgataatac cgccgacaat
    21421 tggcaccacc cagaagaacc acagttgttc taatgcccat ccgccctgga agatagcaac
    21481 cgcagtactg cgcgccgggt taacggaagt gttagtcacc ggaatactga ttaagtgaat
    21541 cagggttaag gccagaccaa tggcaattgg cgcaaaacca gcaggagcga atttgtcagt
    21601 tgcgccgtgg atcaccaaca ggaaacctgc actcaatacc agttcaacta ccagcgcgga
    21661 aagcatggaa taaccgcctg gtgaatgctc gccataaccg ttagaagcaa aaccgctggc
    21721 tgccgcgtca aaacccgttt taccactggc aattaaatac agcagcgccg ccgcaacaat
    21781 accgccgaca acctgggcaa ttacgtagcc aacgacttct tttgccggga aacgtccgcc
    21841 agcccataaa ccaatagtga ccgccgggtt aaaatgacca ccagaaatat gaccaacagc
    21901 aaaggccatt gtcagaacgg tcagaccgaa cgccaacgcc acgccggcaa aaccaatgcc
    21961 taattccggg aagcctgcgg ccagtacggc actaccacag ccgccaaaaa caagccagaa
    22021 agtaccaaaa cattcagctg ctaattttct gaacatatcc acctcaatta aaaattgacc
    22081 ctgtgaaaaa tatggtcgtt ttataggatc gtcgtaaaaa agtgacgacg gaaataatgc
    22141 gcggctattt taaaaacgaa ggcaagtcat tcaccagata aataaatcca gtaaatttga
    22201 tttagggcaa cagcgggttg ccccatatag tcatttgtct gattgacagt gtagtgcaag
    22261 cgaaagattt aatcctttag gcgtaataaa aaataattta tcatgctaat tatttgattt
    22321 tgttgttttt gcagacttat cagcaagagg gagtataacg cgattattcg ctcatttttc
    22381 agacatttgc catgcttaaa tgtgatgtca tcacgtatta gcatggcctt tcccgttata
    22441 ctgccagcgt aaaggataag tcacatattt ctggagggga tatgattctt gagcgcgttg
    22501 aaattgtggg ttttcgcggt atcaaccgtt tgtcgttgat gctggaacaa aacaacgtcc
    22561 tgattgggga gaacgcgtgg ggtaaatcca gcttgctgga cgccttaacc ctgctgctat
    22621 cgccagaatc agatctctac cattttgagc gcgacgattt ctggttcccg ccgggagata
    22681 tcaacgggcg agaacatcat ctgcatatta ttttgacctt ccgcgaatcg ctgccaggcc
    22741 gacatcgggt tcgccgctat cggccgctgg aagcgtgctg gacgccatgc accgatggtt
    22801 atcaccgtat tttttatcgt ctggaagggg agagtgcgga agacggcagc gtgatgacac
    22861 tgcgcagttt tctcgataaa gacggacatc cgattgatgt tgaggatatt aacgatcagg
    22921 ctcgtcatct ggtgcgttta atgccggtgc tgcgcttgcg tgatgcccgt tttatgcgcc
    22981 gtattcgtaa cggcacggtg ccaaatgtcc ccaatgtgga agtcaccgcg cgccagctcg
    23041 atttcctcgc ccgtgaatta tcctcacatc cgcaaaatct ctctgatggg cagattcgtc
    23101 agggactttc cgcaatggtg caacttcttg agcattattt ttctgagcag ggagctggac
    23161 aggcgcgata tcgtttaatg cggcggcgag ccagcaatga gcaacgaagc tggcgctatc
    23221 tggatatcat caaccggatg attgaccgac ctggagggcg ctcgtatcgg gttattttgc
    23281 tcggcctgtt tgctacttta ttgcaggcaa aaggcacatt gcgactggat aaagacgccc
    23341 gtccattgtt gctgatcgaa gatccagaaa cccgtttgca ccccattatg ctttcagttg
    23401 cctggcatct gttgaacctt ctgccattgc agcgcatcgc tactaccaac tctggcgagc
    23461 tgctttcgtt aacgccggta gagcatgttt gccgactggt acgtgagtcc tctcgcgttg
    23521 ccgcctggcg tctgggaccg agtggcttga gtaccgaaga tagccgacgc atctcctttc
    23581 acattcgttt taaccgtccg tcatcgctgt ttgcccgttg ttggctgctg gtggaagggg
    23641 aaacggaaac ctgggttatc aatgaactgg cgcgtcagtg cggacatcat tttgatgccg
    23701 aagggatcaa ggtcattgag tttgcccagt ccgggctaaa gccactggtt aaatttgccc
    23761 gccgaatggg gattgaatgg catgtactgg tcgatggcga tgaagcaggg aagaaatatg
    23821 ccgctacggt acgcagcctg ttgaataacg atcgggaagc cgaacgagaa catttaacgg
    23881 cgttaccggc gctggatatg gaacatttta tgtatcgcca gggattttcc gatgtgttcc
    23941 accgcgtggc gcaaatcccg gaaaatatac cgatgaatct gcgcaaagtt atctcgaaag
    24001 cgatccatcg ctcttccaaa cccgatcttg ccattgaagt ggcaatggag gccggacgtc
    24061 gtggtgtgga ctccgtaccg actctgctga aaaaaatgtt ctcacgcgtg ctgtggctgg
    24121 cgcgcggtcg ggccgactaa ctacaaaaca tcgtggccat ttgtggctga atagcgtcga
    24181 gcatctcata gcgccgacgg tattcagccc gttttttact ggcgatttcg gtaatctctt
    24241 ttcgtgctat ctgtgctgga aggcggtaat ggcgttcagc atcacatacg ccgccaaccg
    24301 attcccagaa agcgttgtaa tcggcatgga tcttgccttc tttatcgcga taacgcaggc
    24361 tgcggtaaat atgcgtttca ttgctgacgg caataatctg ctctacctgc aaacgttggg
    24421 caaacagaca ggccgcttcc atcacgaggc gtttgggaaa tagcccgtgg caggctttcg
    24481 tcgcattctg gatttcctgg tgtggaattt cccattttgc gccttgcagt ccgccaataa
    24541 acatcgtcct tttcccctga tattcacaca gggtaaaagt gagctctgcc agaggaatac
    24601 cttcgctgtt gcggaacagg atggtgctgt caccttcttt atccattgag atcatcatgg
    24661 tcagctcaag cgtgaactgc tcgccgtttt tgccttccag cttcgccagt tgcagcccgg
    24721 gggtattcaa atataagctg aattcttccg ccgacataca cccgcggagt aacgcataat
    24781 ggtaacgtaa cgcctccagc aattgcttac ggctaagatt cgccgcaagg taagggcgat
    24841 gtagacgtac aggcagtcgc ggctggcgcg ttaacaacac attgagatta ggccagtggg
    24901 aaagttcgtt catccactca acgcttaaac gcggcataat caacgagcgc agcaaaaatt
    24961 tctggcgaaa actacggcga tgccagaatt tacccggccg acactgtcct cgtgccagac
    25021 taagaaaaag tgacaggctg ctgagagatt cagatgacgt aaaggtccgt tcagttagct
    25081 gcgacatatt catgaaatca atggttatac atgacgtcga tttaaccatt gcgtatctta
    25141 accaaacatc aatagtgtga ttattaacgt aaattttagg gttttgttga tatttcgttg
    25201 aagttaatga cccggattgg catatggagt attcagaata tttatgaaaa agcggaaaac
    25261 cgtgaagaag cgttacgtta ttgcgatggt gatagtcatc gccgggctga ttacgttatg
    25321 gagaattctt aacgcacccg tgccgactta tcagacgctg attgtgcgcc ccggtgattt
    25381 acagcaaagc gtgctggcga ccggaaagct ggacgcgctg cgtaaggttg acgtgggcgc
    25441 gcaggtcagc ggtcagttga aaacgctgtc ggtggcgatt ggcgataaag taaaaaaaga
    25501 ccagctttta ggggttattg atcctgaaca ggctgaaaac cagatcaagg aggtggaagc
    25561 aacgctgatg gagctacgcg cgcagcggca gcaggcggaa acggagctga aactggcgcg
    25621 ggtgacgtat tcccgtcagc aacgtcttgc acaaacgcaa gcggtttcac agcaggatct
    25681 cgataacgcc gcgacggaga tggctgtgaa acaggcgcaa attggcacca ttgacgcgca
    25741 aatcaagcgc aatcaggcct ctctcgatac ggctaaaacc aatctcgatt acacccgcat
    25801 cgttgccccg atggccgggg aagtcacgca aatcaccact ctgcaaggcc agacggtgat
    25861 tgccgcacaa caagcaccga acattctgac gctggcagat atgagcacca tgctggtaaa
    25921 agcgcaggtt tctgaagcgg atgtaatcca cctgaagccg gggcaaaaag cctggtttac
    25981 ggtgcttggc gatccactga cgcgctacga ggggcaaatc aaggatgtgc taccgacgcc
    26041 ggaaaagatt aacgacgcta ttttctatta cgcccgtttt gaagtaccca accccaatgg
    26101 tttactgcgg ctggatatga ccgcgcaagt gcatattcag ctcaccgatg tgaaaaatgt
    26161 gctgacgatc cctctgtcgg cgttaggcga tccggttggc gataatcgtt ataaagtcaa
    26221 attgttgcgt aatggtgaaa cacgcgagcg tgaagtgacg attggcgcac gtaacgatac
    26281 cgatgttgag attgtcaaag ggctggaagc gggcgatgaa gtggtgattg gtgaggccaa
    26341 accaggagct gcgcaatgac gcccttgctc gaattaaagg atattcgtcg cagctatcct
    26401 gccggtgatg agcaggttga ggtgctgaag ggcatcagcc tcgatattta tgccggtgag
    26461 atggtagcga ttgtcggcgc ttcgggttcc ggtaaatcga ccctgatgaa tattctcggc
    26521 tgcctggata aggccaccag cggcacctat cgcgtcgccg gtcaggatgt tgccacgctg
    26581 gacgccgatg cgctggcgca actgcgccgc gagcatttcg gctttatttt ccagcgttac
    26641 catttgcttt cgcatttaac cgccgagcag aacgttgaag tacccgccgt ctatgccggt
    26701 cttgagcgga aacagcggct gcttcgcgcc caggagttac tgcaacgact ggggctggaa
    26761 gaccgtacag agtattaccc ggcacagctt tcgggtggcc agcaacagcg cgtcagcatc
    26821 gcgcgggcat tgatgaacgg cggtcaggta attcttgccg atgaaccgac cggcgcactg
    26881 gacagccatt ctggcgaaga ggtgatggcg atcttgcatc agctgcgcga tcgcgggcat
    26941 acggtgatta tcgtcaccca cgatccgcag gttgctgctc aggccgagcg ggtgatcgaa
    27001 attcgcgacg gcgaaattgt gcacaatcct cccgccgttg aaaaagtgaa tgctactggc
    27061 gggacggaac ctgttgtcaa cacggcgtct ggttggcggc agtttgtcag cggttttaac
    27121 gaggcgctga cgatggcatg gcgggcgctg gcagcgaata aaatgcgtac tttactgacc
    27181 atgctgggga ttattatcgg tattgcgtcg gtggtttcca ttgtcgtggt gggcgacgcc
    27241 gctaaacaaa tggtgctggc ggatattcgt tctattggta cgaatactat tgatgtctat
    27301 cccgggaaag attttggcga tgacgatccg caatatcagc aggcgctgaa gtacgacgac
    27361 ttaatcgcca tccaaaaaca accgtgggtt gcctctgcca cgcccgccgt ctcgcaaaac
    27421 ctgcgcctgc gttataacaa tgttgatgtg gctgccagtg ccaatggtgt gagcggcgat
    27481 tattttaatg tctatggcat gaccttcagt gaagggaaca cctttaatca ggagcagctg
    27541 aacggtcgtg cgcaggtcgt ggttctcgac agtaatactc gccgccagct tttcccccat
    27601 aaagcagatg tggttggcga ggtgattctg gtcggcaata tgcccgccag agtcattggt
    27661 gtggcggaag aaaaacagtc gatgtttggt agcagtaaag tgctgcgtgt ctggctgcca
    27721 tacagcacga tgtccgggcg agttatgggg cagtcgtggc tgaactccat tactgtcagg
    27781 gtgaaagaag gctttgacag cgctgaggcg gaacagcaac tcacgcgttt actttcactg
    27841 cgccacggta agaaggattt ctttacctgg aacatggatg gcgtcttgaa aaccgttgaa
    27901 aagaccacac gtactttaca actgtttctg acgctggttg cggtgatttc gctggtggtg
    27961 ggcggtattg gtgtaatgaa tattatgctg gtgtcagtga ccgagcggac gcgggaaatt
    28021 ggcattcgca tggctgtagg tgcgcgagca agcgatgttt tgcaacagtt tttgatcgaa
    28081 gccgtactgg tttgcctggt tggtggcgcg ttgggaataa cactgtcact gttaattgct
    28141 ttcaccttgc agcttttctt acccggctgg gagattggtt tttcaccgtt agcgctgctg
    28201 ctggcgtttc tctgctcgac ggtcaccggg attttatttg gctggttacc cgcacgaaat
    28261 gcggcacgac tggatccagt agatgctctg gcacgagagt aatttttgag ataaaaatgc
    28321 cagccgatcg ggctggcatt ttgcctttag gatgtacaca atgagacaga agagctatgc
    28381 gactgccgct tctacttcga cgggcacaat aacactggcg tgattgcctt ttggcccctg
    28441 gtggacatca aactgaacgg attgtccagc ttttagcgtt ctgtaaccat ccatctgaat
    28501 ggtggaataa tgagcgaaaa tatcttcgcc gccgccttca gggcagatga aaccaaaccc
    28561 tttggcattg ttgaaccact taacagtacc cttttccatg cttcgacatc cttcgcaaat
    28621 cttatacaag taagatggaa taaaccgggg tcagagaggg ggctgttcaa aacctcgcca
    28681 actctagaaa tacaatttag agaattaggg cgagccgtca agcatttgac aggggccaga
    28741 gggcaggtat gaatcaaaaa tttgaagcag ttaacgctat tgacaggaat gtgacagatg
    28801 tcgctgatgc taacgataga tgatagttat ctatcatgtg gagtagattg gtcaggcaaa
    28861 taagctcttg tcagcggcag ggcgttctgc cgataaccgt aaccgaagat gataactgac
    28921 aatgggtaaa acgaacgact ggctggactt tgatcaactg gcggaagaaa aagttcgcga
    28981 cgcgctaaaa ccgccatcta tgtataaagt gatattagtc aatgatgatt atactccgat
    29041 ggagtttgtt attgacgtgt tacaaaaatt cttttcttat gatgtagaac gtgcaacgca
    29101 attgatgctc gctgttcact accaggggaa ggccatttgc ggagtcttta ccgccgaggt
    29161 tgccgaaacc aaagtggcga tggtgaacaa gtacgcgagg gagaatgagc atccattgct
    29221 gtgtacgcta gaaaaagcct gaatgcaggc ataaaaattg ggggaggtgc ctatgctcaa
    29281 tcaagaactg gaactcagtt taaatatggc tttcgccaga gcgcgcgagc accgtcatga
    29341 gtttatgacc gtcgagcact tgttactggc gctgctcagt aacccatctg cccgggaggc
    29401 gctggaagcg tgttctgtgg atttggttgc gctccgtcag gaactggaag cctttattga
    29461 acaaaccaca cccgttctgc ctgccagtga agaggagcgc gacacacagc cgacgctgag
    29521 ttttcagcgt gtactgcaac gtgcggtctt ccatgtccag tcctccggtc gcaatgaggt
    29581 taccggtgca aacgttctgg tcgctatctt tagcgaacag gagtcgcagg cggcatatct
    29641 gttgcgcaaa catgaagtca gccgtctcga tgtggtgaac tttatctctc atggcacgcg
    29701 taaagacgag ccgacacagt cttctgatcc tggcagccag ccaaacagcg aagaacaagc
    29761 tggtggggag gaacgtatgg agaatttcac gacgaacctg aatcagcttg cgcgagtggg
    29821 cggaatcgac ccactgattg gtcgtgagaa ggagctggag cgtgctattc aggttctctg
    29881 ccgtcgccgt aaaaacaacc cgctgctggt gggggagtct ggtgtcggta aaaccgcgat
    29941 tgcggagggg cttgcatggc gaattgttca gggcgatgtg ccggaagtga tggctgactg
    30001 tacgatttac tctctcgata tcggttctct gttagcgggc acaaaatatc gcggcgactt
    30061 tgaaaaacgt tttaaagcgt tgctcaagca gctggagcaa gacactaata gcatcctgtt
    30121 tattgatgag atccacacca ttatcggtgc gggtgcagcg tctggtggcc aggtcgatgc
    30181 ggctaacctg atcaaaccgt tgctctccag cggtaaaatc cgcgtaattg gttcgacaac
    30241 ctatcaggag ttcagcaaca tttttgagaa agaccgtgct ctggcgcgtc gcttccagaa
    30301 aattgatatt actgaaccgt caatcgaaga aactgttcaa atcatcaatg gcctgaaacc
    30361 taagtatgaa gcgcaccacg acgtgcgtta taccgcaaaa gcggtgcgtg cggcggtaga
    30421 gctggcggtg aaatacatta acgatcgtca tctgccggat aaagcgattg atgttatcga
    30481 cgaagcgggc gctcgcgcac gtctgatgcc ggtaagcaaa cgcaagaaaa ccgttaatgt
    30541 ggcggatatt gaatccgtgg tggcccgtat tgcacgcatt ccagagaaga gtgtttctca
    30601 gagtgaccgc gataccctga aaaacctcgg cgatcgcctg aaaatgctgg tcttcggtca
    30661 ggataaagcc attgaggcgc tgactgaagc cattaagatg gcgcgtgcag gtttaggtca
    30721 cgaacataaa ccggtcggtt cgttcctgtt tgccggccct actggggtcg ggaaaacaga
    30781 ggtgacggta cagctttcga aagcgttggg cattgagctt ctgcgctttg atatgtctga
    30841 gtatatggaa cgccataccg tcagccgtct gattggtgcg cctccgggat acgttggttt
    30901 tgatcagggc ggtttgctga ctgatgcggt catcaagcat ccacatgcgg tgctgctgct
    30961 ggacgaaatc gagaaagcgc acccggacgt gttcaacatc ctcttgcagg tgatggataa
    31021 cggtacgctg accgataaca acggacgcaa agcggacttc cgtaacgtgg tgctggtgat
    31081 gaccactaac gcgggggtac gtgaaaccga gcgcaaatcc attggtctta tccaccagga
    31141 taacagcacc gatgcgatgg aagagatcaa gaagatcttt acaccggaat tccgtaaccg
    31201 tctcgacaac attatctggt tcgatcatct gtcaaccgac gtgatccatc aggtggtgga
    31261 taaattcatc gtcgagttgc aggttcagtt ggatcagaaa ggtgtttctc tggaagtgag
    31321 ccaggaagcg cgtaactggc tggccgagaa aggttacgac cgggcaatgg gcgcacgtcc
    31381 gatggcgcgt gtcatccagg acaacctgaa aaaaccgctc gccaacgaac tgctgtttgg
    31441 ttcgctggtg gacggcggtc aggtcaccgt cgcgctggat aaagagaaaa atgagctgac
    31501 ttacggattc cagagtgcac aaaagcacaa ggcggaagca gcgcattaat ctgattgtct
    31561 ggtaggttgg tgaagtccgt aatctcgtca ggggttacgg actttttatt tatgggagga
    31621 ggaggttcag accctttttt taatgatgat ggtaagttat tgataattag tgctgcgggt
    31681 aggtaaggat aacaaagggt ggcagcagga gattgagatg gttttgcttt attaacaacg
    31741 ggctaaacgt gtagtatttg agttcactgc cgtacaggca gcttagaaat tcactggtaa
    31801 catactccac ccgcccacca tgttcactgc cgtacaggca gataaaatgc gaaaaaaagc
    31861 ccgtactttc gtacgagctc ttctttaaag atggcggtga gggggggatt gactcgcttc
    31921 gctcgccctg cgggcagccc actcactgcg ttcgtggtct gtccaactgg ctgcgccagt
    31981 tgtcgaaccc cggtcggggc ttctcatccc ccctggagtg caatatgcga aaaaaagccc
    32041 gtactttcgt acgagctctt ctttaaatat ggcggtgagg gggggattcg aacccccgat
    32101 acgttgccgt atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa
    32161 ttgttttgtt gcctgacctc atggggggca acggggcgct actataggga gttggaataa
    32221 aacggtcaag aagaatttta atgataatta ttgtttgctc atactgtaaa caagttgtgc
    32281 agtatatcta catcgagaca agttacggac ttatacttct aaagtactcc atacatatca
    32341 caaaataaaa aggccggcta aaccgacctt ttactcattc tttctcttcg cccatcaggc
    32401 ggtaaaacaa tcagcgacta cggaagacaa tgcggccttt gctcaggtcg tacggggtca
    32461 gttcaacagt cactttgtcg cccgtcagga tgcggatgta gtttttgcgc attttaccgg
    32521 agatgtgtgc agtaaccacg tgaccgtttt ctaactctac gcggaacatg gtattaggca
    32581 acgtttcaag aacggtacct tgcatttcaa tattgtcttc tttggccatc taatcctctg
    32641 gggtatcact accgtaattt gaaccggcaa gataatgccg aagttctgta aataagtaaa
    32701 gatttgcgcg ctaaatcgca acaaacaggt tcggcacatt actccgaaaa cacacggcta
    32761 agccgcacca aaagcgcaac gtataaggga gcggtgagat aaacgatggg cgttacctga
    32821 cgcgaaaaat tccttatcgg cagcggggta atgagcgtaa ccaactctgc gaccgcaatt
    32881 ataacactct ggggagaaat gtgccgaaaa cattcattct tgtggtgaaa acaagcatcg
    32941 aggtacccag aaattattcg gcaatcgtcc gaggcgcatt tgattgagat aattaaggta
    33001 atcccagcgg ggaatttcgc aggcaccaag cgacgctgtg tgatcgttaa ggacctggca
    33061 gtcgataagt ttaccgccat gacggataaa ttcatcacag aagaccagaa gcgccgtttt
    33121 ggacgcattt tccatccggc tgaacatgga ctcgccacaa aatagtgttc cctgggccac
    33181 gccgtacata ccgccgacaa gctcatcttc acgccagact tcaatggagt gggcatgacc
    33241 gagttcgtga aggcgatggt aggcttcgac cacgccacgc gtgatccagg ttccttcttc
    33301 gcgatcgctg gcacagcctt cgatgacctg accaaaagcg taattcatcg tgacacgata
    33361 gggtaagcgt ttatgaaatc gcttcatact acggctgata tgcagtgatt ctggccataa
    33421 caccgcgcgg ggatcgggcg accaccagag gatggggtcg ccaggagaaa accacgggaa
    33481 aataccacgc tgataagcca ttaacaggcg cgcagggcta agatcgcccc ccagtgccag
    33541 caggccgtta ggctcacgta atgcgccttc cggggaaggg aaggctattg aatggcgaga
    33601 aagctgaacc aggcgcatga ccgcaaaact ccacgcaagt cggatcgttc aataatagct
    33661 tacaaaccct gcttaaactg gtaataacgc ccctgtctgg taagcagttc tgcgtgagta
    33721 ccttgctcaa taatttgccc gttgtccatc actattattt gttggaaacg agagagtccg
    33781 cgaagtcgat gggtgaccat taacaccgtt ttctcgcaca tcatttctgc aagcaattca
    33841 aggatctgac tttcggttgt ggcatctaag ccttctgtag gttcatccag caataccagc
    33901 ggcgcatcat gtaacagcgc acgggcgata gccagacggc gcagttcacc accggagagc
    33961 tggcgtccgc cttcacctaa ccaactgttg agacctgcat cctcgagcag cttctccaga
    34021 ccaacgcgac gcaagatctc cgccagagcc tcatcactac taccaggcga ggcgagtaac
    34081 agattatcac gcagcgtggc gctaaacaga tgcactcgct ggggaacaac gctgatggtc
    34141 tgtcgtagag tcgcttcatt caggctggct atggggctat cgttaagcaa aatctcgccc
    34201 tgttgcgggt cccatgcgcg ggtcagcagt tgtaacagtg ttgatttgcc gcatccggtt
    34261 cgcccgagaa tcgctatatg ttctccggcg tttacctgaa gagaaatccc tttaagtgcc
    34321 tgttgggatt gctccggata agtgaactga acatcccgta acgtcagcga aacgcgatcg
    34381 gcaacacgag tttgggtatc agggaaggtg acttccggtt tttgatccgt taaatcagtg
    34441 atccgtacgg cagaggcaat gacttgcccc agatgctgaa atgcacccgt tactggtgcc
    34501 agcgcttcaa acgcggctaa cgcgcagaag acaaacaggg caattaacgc gccgggttga
    34561 gcattgccgc caacgccgcc ggacgccatc cacagcatca ggatcaccgc taacgcgcca
    34621 atgagcagca ttatcgcttg cgacaatgcg gtcagttcag attgacggcg ttgcgcttcc
    34681 agccactgaa tttctgtatt ctctaattgc gtgcgataac gatcgctggc accaaaaatg
    34741 gtcagctcag cttgcccttg cagccaggcc gtcagttgtt ggcgatactg cccgcgaaga
    34801 tgagtcagat tttgcccggt gctttttccc gcacgataaa acagtggtgg catcaggaaa
    34861 agcgtcagta acataatgcc gcccagcgta aaggcgaggg tgaaatcaag gaaacttaac
    34921 ccgattgtca ccaccataat caccacaaaa gcacctacca gcggcgagat aacgcgcagg
    34981 taaagatgat cgagcgtatc aacatccgcc accacgcggt tgagtaattc gccctgacga
    35041 tagcgcgcca gtccggcagg ggagaggggc agcaatttgc tgaaggtgta tatgcgcaga
    35101 tgctgcaaca cgcggaaagt cgcgtcgtga cttaccagac gttcaaaata acgcccggca
    35161 gtacgggtga ttgctgcgcc acgcacgccc gcagcgggta gcatatagtt gaagctgtac
    35221 agtccggcaa cccccgctac cgctgaggcg gagaggaacc agccggaaag ggtcaacaga
    35281 ccgatactgg cgagcagcgt gacaattgcc agcacgatac caagacttaa catccattta
    35341 tgacgtttat acagtgccag atagggtagc aaagcgcgca tttaaatctc ctcctgacga
    35401 tgggccagta atgtggcgaa tgggccgcca gccacactta attccgcgta acgtccttgc
    35461 tcaataatct gaccatcctg cattacccaa atgacatccc agtcagcaag atcttctaac
    35521 tggtgggtga ccattaacgt tgtctggcgc agagaggcgg cattcagcgc ctccattacg
    35581 cgctgttcac tgtgagcatc aaggctggca gcgggttcat ccaacagtaa tagcgaacag
    35641 ggatttagta acgcacgggc caccgccacg cgctgcgcct gccccacgga aaggcgggca
    35701 gcctggtcgc caacaggcgt atcaacgcct tgtggcagga gcggtagaaa ctcactgacc
    35761 caggcgttat ccagcgctgt ttgtaactct tgttcgctgg catcaggtcg cgccagtagt
    35821 acgttatccc gtaatgttgc tgccggtaat tgtgggtttt gcccaaccca ggagagatgt
    35881 ttacgccatg attctggtga taaatcgcgt aattctatcc cgttgattcg tagcgatccc
    35941 tgatatgaga gaaaaccaga aagcgcgttc agcagtgaac ttttacctga accgctgcga
    36001 ccaaccaaca ctgctcgttg gcctgctggc aaagtaaagt tcagcggtcc ggccagcgtt
    36061 ttaccttccg gcgacgtgat aaacagatcc tcggcttcaa tggtcaccgg ctcggtcgat
    36121 gctaattccg cttcaccgcg ctgcggatgg gctagcgggg tttccataaa cgttttcaaa
    36181 ctgtcagctg caccaacagc ctgggcttta gcatgataaa acgtaccgag atcgcgtaat
    36241 ggctggaaaa actctggcgc aaggatcagg gccagaaaac ccgcagccag cgtcacacca
    36301 gtatcgtagt gaccaaaatc cagctcgccg aggtaggaaa aaccaaagta gaccgccacc
    36361 agagcaatcg acagcgaggt aaaaaattcg agaatgccgg aggataaaaa cgccagccgt
    36421 agcacttcca ttgtccgttg gcggaaatct tccgaagcag aacgaatact ttcaatttca
    36481 gcttcaccac gaccaaaaat acgcaatgtt tccatgccgc gcaggcgatc gaggaaatgc
    36541 ccacttaagc gagcaagagc gagaaagtta cgtcggttag catcggcagc ccccattcca
    36601 accagcgcca taaataacgg aattagcggt gcagtgccca gcagaatgag cgccgcggcc
    36661 cagttagagg ggaagatagc caccacaatc agcagcggca ccgacactgc cagcgccatt
    36721 tgcggcaggt agcgtgcata gtaatcatgc atatcgtcaa tttgctcgag taccagcgtc
    36781 gcccagctcc ccgctggttt accctgaatc cacgctggcc ctgcttgttg cagacggtcg
    36841 agaacctgac ggcggatggc aaagcggata tgctgcccgg cgtgataacc cacccgttcg
    36901 cgtaaccaga ccacccatgc gcgcagtaca aaggccagaa acagtaacgt aaagggaagc
    36961 agcagggctt cacggggaat attctccata atcatatgtt gcagaattcg cgccatgaac
    37021 caggcctggg aaatgatcaa tatgccgctc acaaagccca gcagacgaga aatattcagc
    37081 caacgttggg agatgacgct ttgctgtttt aaccagcggg ttaattcttt ttgacgggat
    37141 ttattcattg cacgcttagc aggtgagtta tcagaattat ttgcagagca atgttacaac
    37201 ggggaaaaaa taaaggcgac ccatagtcgc atggtgtcgc cttttttact tttgttactg
    37261 atttgtaaaa ttattttgcg tcagctaaac cgtcgaggta gcgttccgca tcaagagctg
    37321 ccatgcagcc tgtaccagca gatgtaatgg cctggcgata aatgtgatcc atcacgtcgc
    37381 ctgcggcaaa gacgccaggg atgctggtct gggtggcatt accatgaata cccgactgta
    37441 ctttgatgta gccgttttcc agttccagct gcccttcgaa aatggcagta ttcgggctgt
    37501 gaccgatagc aacaaacaga ccggcaacgt cgagtgactc gatgttatcg ctgttttgcg
    37561 tatcgcgcag acgaacgcca gtgacaccca tctgatcgcc ggtcacttct tccagcgtac
    37621 ggttggtgtg cagaataatg ttgccgttct ccactttatc catcagacgc ttaatgagga
    37681 ttttttccgc acggaaacca tcacggcggt gaatcagatg aacttccgaa gcgatgttag
    37741 acagatacag cgcctcttca accgcggtat tgccgccgcc gatgaccgca actttctggt
    37801 tgcgatagaa gaaaccgtcg caggttgcac aagcagaaac tccacggcct ttaaaggctt
    37861 cttcagaggg caggccgaga tagcgtgcag aagctccggt ggcaataatc agcgcgtcgc
    37921 aagtgtattc gccgctatcg ccagtcagac ggaacggaca attctgcaaa tcaactttgt
    37981 tgatgtgatc aaagatgatt tcagtttcaa atttggtggc atgttcgtgc atgcgctcca
    38041 ttaataacgg accggtcaga tcgtttggat cgccaggcca gttttccact tccgtggtgg
    38101 tggtcagttg gccgcctttt tccatgccgg taatcagcac aggttgcagg ttggcgcgcg
    38161 ccgcgtagac agcagcggtg tatcccgccg ggcctgaacc caggataagc agtttactgt
    38221 gtttggtcgt gcccatgaga tccccatagt tgttggcaga caatggcagg attgtaggga
    38281 atttacagac gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca
    38341 tcgataagca ggtcaaattc tcacgtcatt atcacctctg ctacttaaat ttcccgcttt
    38401 ataagccgat taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta
    38461 aaaatcgatg ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa
    38521 aaaaacagta ttcttatatg cgcataacca tgcatgtaaa taccatgttt accgtgctag
    38581 tgaaatctac gtatggcgtg gacagacgcc attcgtgatg tcgatagctg ccacaaggca
    38641 acggtcttct caccgtagac ccaggcattg cgcgccgtga atcttcatga tttcggtcta
    38701 tcgtgacggg tagcgactct gaacagtgat gtttcagggt cagacaggag tagggaagga
    38761 atacagagag acaataataa tggtagatag caagaagcgc cctggcaaag atctcgaccg
    38821 tatcgatcgt aacattctta atgagttgca aaaggatggg cgtatttcta acgtcgagct
    38881 ttctaaacgt gtgggacttt ccccaacgcc gtgccttgag cgtgtgcgtc ggctggaaag
    38941 acaagggttt attcagggct atacggcgct gctgaacccc cattatctgg atgcatcact
    39001 tctggtattc gttgagatta ctctgaatcg tggcgcaccg gatgtgtttg aacaattcaa
    39061 taccgctgta caaaaacttg aagaaattca ggagtgtcat ttagtatccg gtgatttcga
    39121 ctacctgttg aaaacacgcg tgccggatat gtctgcttac cgtaagttac tgggggaaac
    39181 cctgctgcgt ctgcctggcg tcaatgacac acggacatac gttgttatgg aagaagtcaa
    39241 gcagagtaat cgtctggtta ttaagacgcg ctaacacgga acaggtgcaa aatcggcgta
    39301 ttttgattac actcctgtta atccatacag caacagtact ggggtaacct ggtactgttg
    39361 tccgttttag catcgggcag gaaaagcctg taacctggag agcctttctt gagccaggaa
    39421 tacactgaag acaaagaagt cacattgaca aagttaagta gcggccgccg tcttctggag
    39481 gcgttgctga tccttattgt cctgtttgcc gtctggttga tggctgcctt actaagcttt
    39541 aacccttcgg accccagctg gtcgcaaacg gcctggcatg aacctatcca taatttaggt
    39601 gggatgcccg gtgcgtggtt ggcagatacg ctgttcttta tttttggcgt gatggcttac
    39661 accattcccg tcattattgt cggcggttgt tggtttgcct ggcgtcatca gtccagcgac
    39721 gaatacattg attattttgc cgtttcgcta cgcatcattg gcgttttggc gctcatcctt
    39781 acctcctgtg gtctggcggc aatcaacgct gacgatatct ggtattttgc ctccggtggc
    39841 gtcattggca gcttactaag cactacgcta caaccactgc tacacggtag cgggggaact
    39901 attgcgctgc tctgcgtttg ggcagcgggc ctgacgctgt tcaccggttg gtcatgggtg
    39961 accattgctg aaaaactcgg cggctggatt ttaaatattc ttaccttcgc cagtaatcgt
    40021 acccgtcgcg atgatacctg ggtcgatgaa gatgagtatg aagacgacga agagtatgaa
    40081 gatgaaaatc acggcaaaca gcatgaatca cgccgtgccc gtattcttcg cggcgcgcta
    40141 gcgcgtcgta aacggttggc ggaaaaattc attaatccga tggggcggca aacagatgct
    40201 gcgctgttct ccggtaagcg gatggatgat gaagaagaga ttacctacac tgcacgcggt
    40261 gtggctgccg accctgacga cgtcctgttt tcgggcaatc gtgcaacgca gccagaatat
    40321 gacgaatacg atccattatt aaacggtgcg ccaattaccg aacctgttgc tgtagcagct
    40381 gctgctacca cggcgacaca aagctgggct gcaccggttg aacctgtgac tcagacgccg
    40441 ccagttgcct ctgttgatgt tccacctacg caacctacag tagcctggca gcctgtaccg
    40501 gggccgcaaa cgggagaacc ggttattgca cctgcaccgg aaggttaccc acagcagtca
    40561 caatatgcgc agcctgcagt gcaatataat gagccgctgc aacaacctgt acagccacag
    40621 cagccgtatt atgcgcctgc ggctgaacaa cctgtgcaac aaccttacta tgctcctgcg
    40681 gcggaacaac ctgttcagca accgtattac gcccctgcgc cagaacaacc ggtggcaggt
    40741 aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac ataccagacg
    40801 gagcaaactt atcagcagcc agctgctcag gagccgttgt accaacagcc gcaacccgtt
    40861 gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc cacgcgtccg
    40921 ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga acaacttgcg
    40981 gcctggtatc aaccgattcc agaaccggtt aaagaaccgg aaccgatcaa atcttcgctg
    41041 aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt ttctccgctg
    41101 gcatctggtg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt tgccgcgcca
    41161 gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg gattggcccg
    41221 cagttgccac gaccgaaacg catccgcgtg ccaactcgtc gtgaactggc gtcttacggt
    41281 attaagctgc cctcacagcg tgcggcagaa gaaaaagccc gcgaagccca gcgtaatcag
    41341 tacgattctg gcgatcagta caacgatgat gaaatcgatg cgatgcagca ggatgaactg
    41401 gcacgtcagt tcgcccagac gcagcagcaa cgctatggcg aacagtatca acatgatgtg
    41461 cccgtgaaca cagaagatgc agatgctgcg gcagaggcag aactggctcg tcagtttgct
    41521 caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc gttctcgctg
    41581 gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca cgaaccgctg
    41641 tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggtagcacc gcagcagcaa
    41701 tatcagcagc cgcaacaacc ggtagcaccg cagcagcaat atcagcagcc gcaacaaccg
    41761 gtagcaccgc agccgcagta tcagcagccg caacaaccgg ttgcaccgca gccgcagtat
    41821 cagcagccgc aacaaccagt tgcgccgcag ccgcatgata ccctgcttca tccgctgttg
    41881 atgcgcaatg gtgacagccg tccgttgcat aaaccgacga cgccgctgcc ttctctggat
    41941 ttgctgacgc cgccgccgag cgaagtggag ccggtagata cctttgcgct tgaacaaatg
    42001 gcgcgtctgg tggaagcgcg tctggctgat ttccgtatta aagccgatgt cgtcaattat
    42061 tctccggggc cggttatcac tcgctttgaa ttgaacctgg caccgggcgt aaaagcggcg
    42121 cgtatttcta acttgtcacg tgaccttgcc cgttcacttt cgacggtggc ggtgcgtgtc
    42181 gttgaagtta ttcctggcaa accctatgta ggtctggagt taccgaataa aaaacgacaa
    42241 accgtttatc tgcgcgaagt tttggataac gccaaattcc gcgataatcc gtcgccatta
    42301 accgtggtac tgggtaaaga tatcgccggt gagccggtgg ttgccgatct ggcgaaaatg
    42361 ccgcacttgt tggttgcggg gactaccggt tccggtaagt ctgtcggtgt gaacgcgatg
    42421 atcctgagca tgctttataa agcacagcca gaagatgttc gtttcatcat gatcgacccg
    42481 aaaatgctgg agctttcggt ttatgaaggc attccgcacc tgttaacgga agttgttact
    42541 gatatgaaag atgccgccaa cgcgctgcgc tggtgtgtta acgagatgga gcgtcgttat
    42601 aaactgatgt ctgcgctggg tgtgcgtaat ctggcgggtt ataacgaaaa aattgctgaa
    42661 gccgatcgca tgatgcgtcc gattccagac ccgtactgga agccgggtga tagtatggat
    42721 gcgcagcatc cggtgctgaa aaaagaacca tacattgtgg tgttggtgga cgaatttgcc
    42781 gacctgatga tgacggtagg taaaaaagtg gaagagctga tagcacgtct ggcgcaaaaa
    42841 gcccgtgccg cgggtatcca cctcgtactg gcaactcaac gtccatcggt tgatgttatt
    42901 actggtctga ttaaagcgaa tattccgacc cgtatcgcct ttaccgtatc cagtaagatt
    42961 gactcacgta ccattcttga tcaggctggc gcggaatcac tgctgggtat gggggatatg
    43021 ctttactctg ggccgaactc cacgttgccg gtacgtgttc atggtgcttt tgttcgcgat
    43081 caggaagttc atgccgtggt gcaggactgg aaagcgcgtg gccgcccaca gtatgttgat
    43141 ggcatcacct ccgacagtga aagcgaaggt ggcgcgggcg gtttcgatgg cgctgaagaa
    43201 ctggatccgt tgttcgatca ggcggtgcag tttgtcactg aaaaacgcaa agcatcaatt
    43261 tctggcgtac agcgtcagtt ccgcattggt tataaccgtg cagcgcgtat tatcgaacag
    43321 atggaagcgc aggggattgt cagcgaacag gggcacaacg gtaatcgtga agtgctggcc
    43381 ccaccgccgt ttgactaatt aatgcatcgt atgccggaaa aggcgcggta gcgtcgcatc
    43441 cggcactcta tcaactgaaa attcagtatt ttcttctttc ctcaagctga ttattagcct
    43501 ggaatagaga gtagagggag ctcccggtcg ggagtgacgt aatttgagga ataatgatga
    43561 aaaaaattgc catcacctgt gcattactct caagcttagt agcaagcagc gtttgggctg
    43621 atgccgcaag cgatctgaaa agccgcctgg ataaagtcag cagcttccac gccagcttca
    43681 cacaaaaagt gactgacggt agcggcgcgg cggtgcagga aggtcagggc gatctgtggg
    43741 tcaaacgtcc aaacttattc aactggcata tgactcaacc tgacgaaagc attctggttt
    43801 ctgacggtaa aacactgtgg ttctataacc cgttcgttga gcaagctacg gcaacctggc
    43861 tgaaagatgc caccggtaat acgccgttta tgctgattgc ccgcaaccag tccagcgact
    43921 ggcagcagta caatatcaaa cagaatggcg atgactttgt cctgacgccg aaagccagca
    43981 atggcaatct gaagcagttc accattaacg tgggacgtga tggcacaatc catcagttta
    44041 gcgcggtgga gcaggacgat cagcgcagca gttatcagct gaaatcccag caaaatgggg
    44101 ctgtggatgc agcgaaattt accttcaccc cgccgcaagg cgtcacggta gatgatcaac
    44161 gtaagtagag gcacttgagt gagcaatctg tcgctcgatt tttcggataa tacttttcaa
    44221 cccctggccg cgcgtatgcg gccagaaaat ttagcacagt atatcggcca gcaacatttg
    44281 ctggctgtgg ggaagccgtt gccgcgcgct atcgaagccg ggcatctgca ttctatgatc
    44341 ctctgggggc cgccgggtac cggcaaaaca accctcgctg aagtgattgc ccgctatgcg
    44401 aacgctgatg tggaacgtat ttctgccgtc acctctggcg tgaaagagat tcgcgaggcg
    44461 atcgaacgcg ctcggcaaaa ccgcaatgca ggtcgccgca ctattctttt tgttgacgaa
    44521 gttcaccgtt tcaacaaaag ccagcaggat gcatttctgc cacatattga agacggcact
    44581 atcactttta ttggcgcaac cactgaaaac ccgtcgtttg agcttaattc ggcactgctt
    44641 tcccgtgccc gtgtctatct gttgaaatcc ctgagtacag aggatattga gcaagtacta
    44701 actcaggcga tggaagacaa aacccgtggc tatggtggtc aggatattgt tctgccagat
    44761 gaaacacgac gcgccattgc tgaactggtg aatggcgacg cgcgccgggc gttaaatacg
    44821 ctggaaatga tggcggatat ggccgaagtc gatgatagcg gtaagcgggt cctgaagcct
    44881 gaattactga ccgaaatcgc cggtgaacgt agcgcccgct ttgataacaa aggcgatcgc
    44941 ttttacgatc tgatttccgc actgcataag tcggtacgtg gtagcgcacc cgatgcggcg
    45001 ctctactggt atgcgcgaat tattaccgcc ggtggcgatc cgttatatgt cgcgcgtcgt
    45061 tgcctggcaa ttgcttccga agatgtcggc aatgccgacc cacgggcgat gcaggtggca
    45121 attgcggcct gggattgctt tactcgcgtt ggcccggcgg aaggtgaacg cgccattgct
    45181 caggcgattg tttacctggc ctgtgcgcca aaaagcaacg ctgtctacac tgcgtttaaa
    45241 gccgcgctgg ccgatgctcg cgaacgtccg gattatgacg tgccggttca tttgcgtaat
    45301 gcgccgacga aattaatgaa ggaaatgggc tacgggcagg aatatcgtta cgctcatgat
    45361 gaagcaaacg cttatgctgc cggtgaggtt tacttcccgc cggaaatagc acaaacacgc
    45421 tattatttcc cgacaaacag gggccttgaa ggcaagattg gcgaaaagct cgcctggctg
    45481 gctgaacagg atcaaaatag ccccataaaa cgctaccgtt aatgttatcg ttgcggtaat
    45541 gttgttaatg tatccctgtg gtcgcaggct gtggccacat ctcccattta attcgataag
    45601 cacaggataa gcatgctcga tcccaatctg ctgcgtaatg agccagacgc agtcgctgaa
    45661 aaactggcac gccggggctt taagctggat gtagataagc tgggcgctct tgaggagcgt
    45721 cgtaaagtat tgcaggtcaa aacggaaaat ctgcaagcgg agcgtaactc ccgatcgaaa
    45781 tccattggcc aggcgaaagc gcgcggggaa gatatcgagc ctttacgtct ggaagtgaac
    45841 aaactgggcg aagagctgga tgcagcaaaa gccgagctgg atgctttaca ggctgaaatt
    45901 cgcgatatcg cgctaaccat ccctaacctg cctgcagatg aagtgccggt aggtaaagac
    45961 gaaaatgaca acgttgaagt cagtcgctgg ggcaccccgc gtgagtttga ctttgaagtt
    46021 cgtgaccatg tgacgctggg tgaaatgcac tctggcctcg actttgcagc cgcagttaag
    46081 ttgactggtt cccgctttgt cgtcatgaaa gggcagattg ctcgtatgca ccgcgcactg
    46141 tcgcagttta tgctggatct gcataccgaa cagcatggct acagtgagaa ctatgttccg
    46201 tacctggtta accaggacac gctgtacggt acgggtcagc tgccgaaatt tgctggcgat
    46261 ctgttccata ctcgtccgct ggaagaagaa gcagacacca gtaactatgc gctgatccca
    46321 acggcagaag ttccgctgac caacctggta cgcggtgaaa tcatcgatga agatgatctg
    46381 ccaattaaga tgaccgccca caccccatgt ttccgttctg aagctggttc atatggtcgt
    46441 gacactcgtg gtctgatccg tatgcaccag ttcgacaaag ttgaaatggt gcagatcgtg
    46501 cgcccggaag actcaatggc ggcactggaa gagatgaccg gtcatgcgga aaaagtcctg
    46561 cagctgctgg gcctgccgta ccgcaaaatc atcctttgta ccggcgacat gggctttggt
    46621 gcttgcaaaa cttacgacct ggaagtatgg atcccggcac agaacaccta ccgcgagatc
    46681 tcttcctgct ccaacgtctg ggatttccag gcgcgtcgta tgcaggcacg ttgccgcagc
    46741 aagtcggaca agaaaacccg tctggttcat accctgaacg gttctggtct tgctgttggt
    46801 cgtacgctgg ttgcagtaat ggaaaactat cagcaggctg atggtcgtat tgaagtacca
    46861 gaagttctgc gtccgtatat gaacggactg gaatatattg gctaataccc aatttttctg
    46921 aatctaaaaa gcgcctgcgg gcgctttttt tgtctccctt tgataccgaa caataattac
    46981 tcctcactta caagtaatac tactttcgag tgaaaatcta cctatctctt tgattttcaa
    47041 attattcgat gtatacaagc ctatatagcg atctgctata gaaataatta cacaatacgg
    47101 tttgttactg gaatcaatcg tgagcaagct tgagtgagcc attatgaaaa cgaaaatccc
    47161 tgatgcggta ttggctgctg aggtgagtcg ccgtggtttg gtaaaaacga cagcgatcgg
    47221 cggcctggca atggccagca gcgcattaac attacctttt agtcggattg cgcacgctgt
    47281 cgatagcgct attccaacaa aatcagacga aaaggttatc tggagcgcct gtacagttaa
    47341 ctgtggtagt cgctgcccgc tacgtatgca cgtagtggac ggtgaaatca aatatgtcga
    47401 aacggacaat accggcgatg acaattacga cggcctgcac caggttcgcg cctgtctgcg
    47461 tgggcgttcc atgcgtcgcc gtgtctacaa tccggaccgc ctgaaatatc cgatgaaacg
    47521 agtcggggcg cgcggtgaag gcaaattcga gcgcattagc tgggaagaag cctacgacat
    47581 catcgcgacc aaaatgcagc gcctgatcaa agagtacggc aacgagtcta tctatctgaa
    47641 ctatggcacc ggtacgctgg gcggcaccat gacccgctcg tggccgccgg gaaataccct
    47701 ggtcgcgcgg ctaatgaact gctgcggcgg ttatctgaac cattatggcg actactcctc
    47761 cgcgcaaatt gcggaaggtc tgaactatac ctacggcggc tgggcagatg gtaacagccc
    47821 gtcggatatc gaaaacagta aactggtagt gctgtttggt aataaccctg gcgaaacgcg
    47881 aatgagtggc ggtggggtga cttactatct tgaacaggcg cgcaagaaat ctaatgctcg
    47941 catgatcatc atcgatccgc gctataccga caccggtgcc gggcgtgaag atgagtggat
    48001 ccctattcgt ccgggaacag atgccgcact ggttaacggt ctggcgtatg tcatgatcac
    48061 cgaaaacctg gtagatcagg aattcctcga taaatattgc gttggctacg acgagaaaac
    48121 cctgccagcc agtgcgccga aaaatggtca ctataaagct tatattctgg gtgaaggacc
    48181 cgatggcgtg gctaaaacac cggaatgggc ctcgcaaatc accggtgttc cggcagacaa
    48241 aatcatcaaa ctggctcgtg aaatcggcag taccaaaccg gcgtttatca gccagggatg
    48301 gggcccgcag cgtcacgcta acggtgaaat cgcaacccgt gctatctcga tgctggcgat
    48361 tctgaccggt aacgttggta ttaacggggg gaacagcggc gcgcgcgaag gttcatacag
    48421 cttaccgttt gtccgtatgc cgactctcga aaacccgatc cagaccagca tttctatgtt
    48481 tatgtggacc gatgccattg aacgtggccc ggaaatgacg gcgctgcgtg atggtgtgcg
    48541 cgggaaagat aagctggatg tgccgatcaa aatgatctgg aactatgccg gtaactgcct
    48601 gattaatcag cattctgaaa tcaaccgtac ccatgaaatc cttcaggatg ataagaagtg
    48661 cgagctgatt gtggttatcg actgccacat gacctcatcg gcgaaatatg ctgacatcct
    48721 gctgcctgac tgcaccgctt ccgaacagat ggactttgca ctggatgcat cctgcgggaa
    48781 tatgtcttac gttattttca atgatcaggt cattaaaccg cgctttgaat gtaagactat
    48841 ctatgagatg accagcgaac tagcaaaacg tcttggcgtc gaacaacagt ttactgaagg
    48901 tcgtactcag gaagagtgga tgcggcatct gtatgcccag tcgcgggaag cgattcctga
    48961 actgccaacg tttgaagagt tccgtaagca ggggatcttt aaaaagcgcg acccacaagg
    49021 gcatcacgtt gcttataaag ctttccgtga agatccgcag gcaaatccac tgaccacgcc
    49081 atcggggaaa attgagatct attcacaggc gctggctgac attgccgcca cttgggaatt
    49141 gccagaaggt gatgtgatcg atccactgcc gatctatacg ccaggctttg aaagttatca
    49201 ggatccgctg aacaaacagt atccgctgca gcttacgggt ttccactata aatctcgcgt
    49261 tcactcaact tacggcaacg ttgatgtgct gaaagcagct tgccgtcagg aaatgtggat
    49321 caacccgctt gatgcgcaaa aacgcggtat cagcaacggc gataaagtca ggatctttaa
    49381 tgatcgtggt gaggttcata ttgaggcgaa agtgacgcca cgaatgatgc cgggtgtggt
    49441 cgcactgggt gaaggtgcct ggtatgaccc ggatacaaaa cgtgtcgata agggcggttg
    49501 tattaacgta ctgaccactc aacgtccgtc tcctctcgct aaggggaatc cgtcacatac
    49561 aaaccttgtt caggttgaaa aggtgtaagg agtaaccgat gacaacccag tatggatttt
    49621 ttattgattc cagccgttgc accggttgca aaacctgcga gctggcctgt aaagactaca
    49681 aagatctgac gccggaagtt agtttccgcc gcatttatga atatgctggt ggagactggc
    49741 aggaagataa cggcgtctgg catcaaaacg tatttgccta ctatttgtcg atttcatgca
    49801 accactgcga agatccggct tgtaccaaag tctgcccaag cggtgcgatg cataaacgtg
    49861 aagatggttt tgtcgttgtc gatgaagatg tgtgtattgg ctgtcgttac tgccatatgg
    49921 cctgcccgta tggcgcaccg cagtacaacg caaccaaagg tcatatgacc aaatgtgatg
    49981 gttgctatga ccgcgtctca gaaggtaaaa aaccgatctg cgtcgaatct tgcccgctac
    50041 gggcgctgga tttcggacct atcgacgagc tgcgtaaaaa acacggcgat ctggcggctg
    50101 ttgcgccgtt gccgcgagct cactttacca aaccgaatat tgtgatcaaa cccaatgcca
    50161 acagccgccc aaccggggat accactggct atctggcaaa cccgaaggag gtgtgagatg
    50221 ggaagtggat ggcatgaatg gccgctgatg atcttcacgg tcttcgggca atgtgtagcg
    50281 ggtggtttta tcgttctggc tttggcgctg ctcaaaggcg acctgcgagc agaatcccag
    50341 cagcgtgtta tcgcctgcat gtttggttta tgggtgctga tgggcattgg ctttatcgcc
    50401 tctatgcttc atcttggttc accgatgcgc gcttttaact cgctcaatcg ggtaggggct
    50461 tcagcactca gtaacgaaat cgccagcggt tcgatctttt ttgctgtagg cggcatcggc
    50521 tggttgctgg caatgctgaa aaagctttca ccggcattgc gtacgctttg gctggtggtg
    50581 acgatggttc ttggcgtagt ctttgtctgg atgatggtgc gtgtgtataa cagcatcgat
    50641 acagtaccca cctggtacag catctggaca ccgatgggct tcttcctgac gatgtttatg
    50701 ggcggcccgt tactgggtta cctgttgctg agtctggctg gtgtcgatgg ctgggcgatg
    50761 cgtctgctgc cagccatttc tgttctggca ctggtagtga gtggcgtgat gtcagtgatg
    50821 cagggcgcag agctggcgac tattcatagc tctgtgcagc aggccgcagc gctggtgccg
    50881 gactatggtg cgctgatgtc ctggcggatc gtgcttttgg ccgttgccct gtgcttgtgg
    50941 attgcaccac agctaaaagg ttatcagcct gcggttccgc tactttctgt atcattcatt
    51001 ctgcttctgg caggggaatt aatcggtcgt ggcgtattct atggtttgca tatgaccgtg
    51061 gggatggctg tcgcaagtta atcagtaaca accggggttt cggccccggt tcgcttttat
    51121 ttctgcttcg ttaacgtgtc ataactggtc atcaagttac gataatcagg aatgtggtta
    51181 gagaacaacg tcgccagtcc ttcaatatca ttacgccagt cgcgatgcag ctcgcacgcc
    51241 acgccgaacc atgtcatcaa ttgcgcacca gcttgcgaca tgcgatccca tgccgaatgc
    51301 cgggtaattt cattaaaggt ccctgacgcg tcagtcacga caaagacgtc aaacccttct
    51361 tcaatggctg aaagcgccgg gaatgccacg caaacttcgg ttaccacacc ggcaataatt
    51421 aactgttttt tacctgtcgc tttgacagct tttacaaaat cttcgttatc ccaggcgtta
    51481 atatttcccg ggcgagcaat gtaaggtgca tcggggaatt gtgctttcag ttctggaact
    51541 aatgggccgt taggaccggt ttcaaaactg gtggtaagaa tagttggtaa gttgaaatac
    51601 ttagccaggt cacccagcgc cagcacgtta tttttaaact tatcgggttc gatatcccgt
    51661 acaagggaaa gtaaaccagc ctggtgatca acaagcaaaa cggcagcatc atttttatca
    51721 agacgaacat acggtttggt catcctcttc tcctttcgag agtcagtggt acgtctaaat
    51781 cggtcagacg tgatgcattg ctctgaaagc atagacggga aatatgagtt tgctgtgacc
    51841 atgaaatttt tcgactgaac gaagcgtatg aaatttgtgt tagatcaata aaaacaatca
    51901 gatgagatta atcgtctctt cagcattggc gttatgaatg agtaatataa atcgataata
    51961 ccgccgctgc gatatgcgcg tattttcacc cattgacaat gtttttggcg gtggcatgat
    52021 gcgcatgaaa tttgaacttc ctcacggttt taattcatgt ccacgtatac ccggcctgtc
    52081 atgcttttgc tgtctggcct gcttttgttg actctggcga tcgcggtgtt aaatacactc
    52141 gtgccgcttt ggctcgccca ggaacacatg tctacatggc aggtaggcgt tgtcagctca
    52201 tcctatttta ccggcaacct tgtcggtaca ttgctgacag ggtatgtcat taaacgcatt
    52261 ggctttaacc gtagctatta tctggcctcc ttcatttttg ccgctggctg tgccggcctt
    52321 ggcctgatga ttggattctg gagttggttg gcgtggcgtt ttgtcgctgg catcggctgt
    52381 gccatgattt gggtggttgt tgagagtgcg ctgatgtgca gtgggacgtc gcgtaaccgc
    52441 gggcggttgc ttgctgcgta tatgatggtt tattacgtcg gaacgttttt aggccagtta
    52501 ctggtcagca aagtctcaac cgagttgatg agtgtattgc cgtgggttac aggtttgacg
    52561 ttggcaggaa tcttaccgct gttgtttacg cgtgtcctga atcagcagac tgaaaaccat
    52621 gattcgacgt caattacgtc aatgctaaaa ctccgtcagg cgcggcttgg cgtgaatggc
    52681 tgcattatct caggaatcgt gctgggatct ctatatggcc tgatgccgct gtacctcaat
    52741 cacaaagggg tgagcaatgc cagcattggt ttctggatgg cggtactggt cagtgcgggt
    52801 atcctcgggc aatggccgat tggacgtctg gcggataagt ttggtcgact gctggtgttg
    52861 cgtgttcagg tctttgtcgt cattctcggc agtattgcga tgcttagcca ggcggcgatg
    52921 gccccagcgt tattcatcct cggtgccgct ggctttacgc tatatccggt ggcgatggcc
    52981 tgggcttgtg agaaagttga acatcatcaa ctggtggcga tgaaccaggc cttactgttg
    53041 agctataccg tgggaagcct gcttggcccg tcatttaccg ctatgctaat gcagaatttc
    53101 tccgataatt tattatttat catgatcgcc agcgtatcgt ttatctattt gctgatgctg
    53161 cttcgtaacg ctggtcatac gccgaaaccc gtggctcacg tgtaaatgaa ttcaagcaga
    53221 gtgtgaactt actgtttcac actctgcttt tctgtgcaca aaaaagcggt aactcaataa
    53281 agttgccgct ttacggggaa attagaacat gaccttatga ccgtactgct caagaatgcc
    53341 tttcacgcgt tccatggtct ctttcttcgg tggtttaaca ccatcgagtt tgtattcttc
    53401 acccatcgcc acccatttgt gtttgcccag ttcgtggtag gggaggagct cgattttctc
    53461 aacgttgccc atatcacggg taaattcacc aaggcgatgc gctgaatcgt catcgtcaga
    53521 ccagcctggg acaacaacat agcggatcca caccttcaca tttttgttcg ccagatattt
    53581 agcgaactcc agcgtgcggt ggttggaaac tccaaccaga ttttggtgga tctcgtcgtt
    53641 catctgtttg agatcgagca ttaccaggtc ggttacttcc agcagttcat caatcaccgg
    53701 atcgtaacga cgaacaaaac cgttggtgtc cagacaggta tgaatgcctt cttttttgca
    53761 ggcgcggaac cagtcacgaa caaactcagc ttgtaggatt gcctcaccgc cggatgcggt
    53821 aacgccgccg ccggaagcgt tcataaagtg gcgataggtc accacttcct tcatcaaatc
    53881 ttcaacggta acttctttac cgccatgcgt atcccaggtg tcgcggttat gacaatacag
    53941 gcagcgcatc aggcagccct ggaaaaaggt gataaagcga atacccgggc cgtctacggt
    54001 tccacaggat tcaaaggagt gaatgcgacc aataactgac attgcggtgt ttctccagat
    54061 gtggcccatc tgaggccgtg ttggtgcgca gctcgaaggc tacgtcgagt ctgttttggc
    54121 agtcacctta aagtatagat agctgacaaa aaaggctctc gtgctaaaaa aggccccact
    54181 ttcgtggagc ctttattgta cgctttttac tgtacgattt cagtcaaatc taattacata
    54241 gattgagtga aggtacgagt aataacgtcc tgctgctgtt ctttagtcag cgagttgaaa
    54301 cgtactgcgt agccagatac acggatggtc agctgcggat atttttccgg gttttccatc
    54361 gcgtcgagca gcatttcacg gttcatcacg ttaacgttca ggtgctgacc accttcgatg
    54421 gatgcttcgt ggtggaagta accatccatc agaccagcca ggttggtctt acgaacttcg
    54481 tcgtctttac ccagtgcgtt cggaacgata gagaaggtgt aggagatacc atctttagcg
    54541 taagcaaacg gcagtttagc aacggaagtc agagacgcta cagcaccttt ctggtcacga
    54601 ccgtgcatcg ggttagcacc cggtccgaac ggcgcgccag cacgacgacc gtctggggtg
    54661 ttaccagttt tcttaccata cacaacgtta gaagtgatgg tcagaacaga ctgagtcggg
    54721 atagcgtcac ggtaggtgtg cagtttctga attttcttca tgaaacgttc taccaggtca
    54781 acagccaggt catctacacg cggatcgttg ttaccaaact gcgggtattc gccttcgatt
    54841 tcgaagtcga tagccagacc gtcttcgtca cgaatcggtt taactttcgc atatttgatt
    54901 gcagacaggg agtcagcagc aacggacaga ccagcgatac cacacgccat ggtgcggata
    54961 acgtcacggt cgtgcagcgc catcagagag gcttcgtagc tgtacttgtc gtgcatgtag
    55021 tggatgatgt tcagtgcagt gatgtactgt ttagccagcc agtccatgaa gtgatccatg
    55081 cgctccatca cttcatcata gttcaggaca tcgcctttga tcggttcaga cttcggacca
    55141 acctgcattt tcagtttttc gtcaacgccg ccgttgattg cgtacagcat ggttttcgcc
    55201 aggtttgcac gcgcaccgaa gaactgcatt tgtttaccaa cgatcatcgg gcttacgcag
    55261 caagcaatag cgtagtcatc gttgttgaag tccggacgca tcaggtcatc gttctcatac
    55321 tgcagagaag aggtgtcgat ggacacttta gcggcgaatt tcttgaagtt cagcggcagt
    55381 ttttcagacc acagaatggt catgttcggt tccggagacg gacccatggt gtacagggtg
    55441 ttcaggaaac ggaagctgtt tttggtaacc agggtacgac cgtcgaggcc cataccaccg
    55501 atagattcgg ttgcccagat cgggtcgcca gaaaacagtt catcgtattc cggagtacgc
    55561 aggaagcgaa ccatacgcag tttcatgacc aggtggtcaa ccatttcctg cgcttcttgt
    55621 tcggtgatct tgccagcttt caggtcacgt tcgatgtaca catccaggaa ggtggaggta
    55681 cgaccgaagg acattgcagc accgttctga gacttaacag cagccaggta gccaaagtaa
    55741 gtccactgga tagcttcctg agcgttggta gccggaccag agatgtcgta gccgtatttc
    55801 gcagccattt ctttcatctg acccagagcg cggtgctgtt cagcgatttc ttcgcgcaga
    55861 cggatagtct gttccaggtt tacgccgttt tccagatcag cctgcagaga ggtgaactgt
    55921 gcaagtttgt ctttcatcag gtagtcgata ccgtacagcg caacgcgacg gtagtcaccg
    55981 atgatacgac cacggccata agcatctggc agaccggtca gaacaccgga tttacggcaa
    56041 cgcaggatgt ccggagtgta aacgtcgaac acgccctggt tgtgagtttt acggtattca
    56101 gtgaagattt ttttgatcat cgggtccagt tcgcggttgt acgctttgca ggaaccttcg
    56161 atcattttga taccaccgaa cgggataaga gcacgtttca gcggagcttc agtctgcaga
    56221 ccaacgattt tctcaagctg cttgttgatg taaccagcgt cgtgagaggt gatggtggaa
    56281 gcaacagcgg tgtcaaagtc aactggcgcg tgagtgcggt tttccagttt aacgccttcc
    56341 attactttgt cccacagggt ggtggtcgct tcagtagcgc cagccaggaa ggactcgtca
    56401 ccctcgtacg gagtgtagtt tttctgaatg aagtcacgga cgtttacttc attctgccag
    56461 tcacctttgg taaaaccttc ccaggctgtg gctaactttt cattaagctc ggacatgtaa
    56521 cacctacctt cttaagtgga ttttttattt actgcgtact tcgacaacca ttaatggtga
    56581 tcgttttcac gcaggtaaat gacccagtat gtcaacccaa ccaacaaacc accgccgata
    56641 atattaccga tcgtaaccgg aatcaggtta tcagtgatga agttcatcac ggtcaggtga
    56701 gaaaaatttt ccggtgcaga accgacagcg gtccagaatt ccggagatgc gaagtcgcgg
    56761 attacaatac ccatcgggat cataaacatg tttgcgatac tgtgctcaaa accgctggca
    56821 acaaacatcg cgaccggcag caccataatg aacgctttgt ccatcaggct gcggccagaa
    56881 taactcatcc atactgccag acataccatc aggtttgcca ggataccaag acagacggcc
    56941 tcaataaaag tatggtgcac tttgtggtcg gcggtttgta ggacgtttag tccccattga
    57001 ccatttgcgg tcatatactc gccggaaagc cacattaaaa gtacaaacag cagtgcgccg
    57061 accaggttgc caaaataaac atttagccag tttttcgcca actgacccca ggtgatgcgc
    57121 ccactcgcct tggcaacaac aatcaacact gtggaagtaa agagatcggc tccgcagaca
    57181 acacaaagaa tcaaccccag agagaagcaa atgccgccaa tcagttttgc catgccgaag
    57241 ggcattgtac ctgtgccagt ggttgctgtg atatagaaga cgaatgcgat tgagatgaaa
    57301 acaccggcgg taatcgccag atagaaagtc ttaagcggat gtttcgttgc tttatagaca
    57361 cctgcctctt cggccacttt ggccattgca gcaggaagta aaagatcaaa agggttgtca
    57421 gctttcacac taactctctc tttattaagt cggcgacgag atactaacaa agcattatag
    57481 atgagaaatt gatatagatc atatctcgcc tggc
//
LOCUS       DAZFJL010000012        57395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFJL010000012 DAZFJL010000000
VERSION     DAZFJL010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.012"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            193..819
                     /gene="ydhY"
                     /locus_tag="ACHJ8C_000726"
     CDS             193..819
                     /gene="ydhY"
                     /locus_tag="ACHJ8C_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO5550632.1"
                     /translation="MNPVDRPLLNIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            840..2942
                     /gene="ydhV"
                     /locus_tag="ACHJ8C_000727"
     CDS             840..2942
                     /gene="ydhV"
                     /locus_tag="ACHJ8C_000727"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO5550633.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKSFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFIYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEDMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            2955..3593
                     /gene="ydhW"
                     /locus_tag="ACHJ8C_000728"
     CDS             2955..3593
                     /gene="ydhW"
                     /locus_tag="ACHJ8C_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO5550634.1"
                     /translation="MNHQDELPLAEVSEIDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIATMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3657..4325
                     /gene="ydhX"
                     /locus_tag="ACHJ8C_000729"
     CDS             3657..4325
                     /gene="ydhX"
                     /locus_tag="ACHJ8C_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO5550635.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNGTQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAETRLA
                     KGFPPICVSACPEHALIFGHEDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4322..5107
                     /gene="phsC"
                     /locus_tag="ACHJ8C_000730"
     CDS             4322..5107
                     /gene="phsC"
                     /locus_tag="ACHJ8C_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO5550636.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPQFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSATDHGERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDMFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5111..5923
                     /gene="ydhT"
                     /locus_tag="ACHJ8C_000731"
     CDS             5111..5923
                     /gene="ydhT"
                     /locus_tag="ACHJ8C_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO5550637.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERLFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGARARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANCGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGCAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(5935..7539)
                     /gene="ydhS"
                     /locus_tag="ACHJ8C_000732"
     CDS             complement(5935..7539)
                     /gene="ydhS"
                     /locus_tag="ACHJ8C_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO5550638.1"
                     /translation="MKTIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDDENSKLMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNLDLPSETFDLVVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVTFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIITDQALNAEIQKG
                     EVGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEENL
                     QEVERNKREKHTVPWRYVILRMHEAVQEIVPYLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHLLALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRQLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7665..7970)
                     /gene="ydhR"
                     /locus_tag="ACHJ8C_000733"
     CDS             complement(7665..7970)
                     /gene="ydhR"
                     /locus_tag="ACHJ8C_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO5550639.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFSDEKSALTYLEKHTARLKNLGVEEVVAKVFDVNVSLSQINQAKLA"
     gene            complement(8078..8154)
                     /locus_tag="ACHJ8C_000734"
     tRNA            complement(8078..8154)
                     /locus_tag="ACHJ8C_000734"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8118..8120),aa:Val,seq:gac)
     gene            complement(8159..8235)
                     /locus_tag="ACHJ8C_000735"
     tRNA            complement(8159..8235)
                     /locus_tag="ACHJ8C_000735"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8199..8201),aa:Val,seq:gac)
     gene            8543..9799
                     /gene="ydhQ"
                     /locus_tag="ACHJ8C_000736"
     CDS             8543..9799
                     /gene="ydhQ"
                     /locus_tag="ACHJ8C_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO5550640.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCAQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEACNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITDAATQVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNSLKLQPDQDGIGATLQPA"
     gene            complement(9840..11213)
                     /gene="mdtK"
                     /locus_tag="ACHJ8C_000737"
     CDS             complement(9840..11213)
                     /gene="mdtK"
                     /locus_tag="ACHJ8C_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO5550641.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRASR"
     gene            11428..12069
                     /gene="ribE"
                     /locus_tag="ACHJ8C_000738"
     CDS             11428..12069
                     /gene="ribE"
                     /locus_tag="ACHJ8C_000738"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO5550642.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12109..13257)
                     /gene="cfa"
                     /locus_tag="ACHJ8C_000739"
     CDS             complement(12109..13257)
                     /gene="cfa"
                     /locus_tag="ACHJ8C_000739"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO5550643.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13548..14759)
                     /gene="punC"
                     /locus_tag="ACHJ8C_000740"
     CDS             complement(13548..14759)
                     /gene="punC"
                     /locus_tag="ACHJ8C_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO5550644.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLFIGLTIFALGSLGMLWVENAATLLILRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14872..15804
                     /gene="punR"
                     /locus_tag="ACHJ8C_000741"
     CDS             14872..15804
                     /gene="punR"
                     /locus_tag="ACHJ8C_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO5550645.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERIRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15801..16826)
                     /gene="purR"
                     /locus_tag="ACHJ8C_000742"
     CDS             complement(15801..16826)
                     /gene="purR"
                     /locus_tag="ACHJ8C_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO5550646.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQSHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17125..17214
                     /gene="cydH"
                     /locus_tag="ACHJ8C_000743"
                     /gene_synonym="ynhF"
     CDS             17125..17214
                     /gene="cydH"
                     /locus_tag="ACHJ8C_000743"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO5550647.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17380..18549
                     /gene="ydhP"
                     /locus_tag="ACHJ8C_000744"
     CDS             17380..18549
                     /gene="ydhP"
                     /locus_tag="ACHJ8C_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5550648.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLASIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLAHNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18695..19276)
                     /gene="sodB"
                     /locus_tag="ACHJ8C_000745"
     CDS             complement(18695..19276)
                     /gene="sodB"
                     /locus_tag="ACHJ8C_000745"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO5550649.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19404..20231)
                     /gene="mepH"
                     /locus_tag="ACHJ8C_000746"
     CDS             complement(19404..20231)
                     /gene="mepH"
                     /locus_tag="ACHJ8C_000746"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO5550650.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTQKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNATSGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20566..20913
                     /gene="grxD"
                     /locus_tag="ACHJ8C_000747"
     CDS             20566..20913
                     /gene="grxD"
                     /locus_tag="ACHJ8C_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO5550651.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21130..21192)
                     /locus_tag="ACHJ8C_000748"
                     /pseudo
     CDS             complement(21130..21192)
                     /locus_tag="ACHJ8C_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
     gene            complement(21285..21932)
                     /gene="rnt"
                     /locus_tag="ACHJ8C_000749"
     CDS             complement(21285..21932)
                     /gene="rnt"
                     /locus_tag="ACHJ8C_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO5550652.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(22035..22442)
                     /gene="gloA"
                     /locus_tag="ACHJ8C_000750"
     CDS             complement(22035..22442)
                     /gene="gloA"
                     /locus_tag="ACHJ8C_000750"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO5550653.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTNVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(22523..23620)
                     /gene="nemA"
                     /locus_tag="ACHJ8C_000751"
     CDS             complement(22523..23620)
                     /gene="nemA"
                     /locus_tag="ACHJ8C_000751"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO5550654.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELGEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(23657..24256)
                     /gene="nemR"
                     /locus_tag="ACHJ8C_000752"
     CDS             complement(23657..24256)
                     /gene="nemR"
                     /locus_tag="ACHJ8C_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO5550655.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            24359..24598
                     /gene="ydhL"
                     /locus_tag="ACHJ8C_000753"
     CDS             24359..24598
                     /gene="ydhL"
                     /locus_tag="ACHJ8C_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO5550656.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRVNKSAPSDEPEQPSLF"
     gene            24647..25543
                     /gene="ydhF"
                     /locus_tag="ACHJ8C_000754"
     CDS             24647..25543
                     /gene="ydhF"
                     /locus_tag="ACHJ8C_000754"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO5550657.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTACEENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRIRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            25624..26145
                     /gene="sodC"
                     /locus_tag="ACHJ8C_000755"
     CDS             25624..26145
                     /gene="sodC"
                     /locus_tag="ACHJ8C_000755"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO5550658.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(26146..28158)
                     /gene="ydhK"
                     /locus_tag="ACHJ8C_000756"
     CDS             complement(26146..28158)
                     /gene="ydhK"
                     /locus_tag="ACHJ8C_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO5550659.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWRPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPDATREIL
                     EQLLTALASSQTDVYTVARIISPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDRARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(28158..29015)
                     /gene="ydhJ"
                     /locus_tag="ACHJ8C_000757"
     CDS             complement(28158..29015)
                     /gene="ydhJ"
                     /locus_tag="ACHJ8C_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO5550660.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNFYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNYISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHASSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFTLDKVPSDVTLVSG
                     TTCSIAVGQ"
     gene            complement(29018..29254)
                     /gene="ydhI"
                     /locus_tag="ACHJ8C_000758"
     CDS             complement(29018..29254)
                     /gene="ydhI"
                     /locus_tag="ACHJ8C_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO5550661.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVIHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            29455..29889
                     /gene="slyA"
                     /locus_tag="ACHJ8C_000759"
     CDS             29455..29889
                     /gene="slyA"
                     /locus_tag="ACHJ8C_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO5550662.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(29936..30403)
                     /gene="slyB"
                     /locus_tag="ACHJ8C_000760"
     CDS             complement(29936..30403)
                     /gene="slyB"
                     /locus_tag="ACHJ8C_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO5550663.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRQVQIQGGDDSNVIGAIGGAVLGGFLGNTIGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTHFSPGQRVVLASNGSQVTVSPR"
     gene            30677..31786
                     /gene="anmK"
                     /locus_tag="ACHJ8C_000761"
     CDS             30677..31786
                     /gene="anmK"
                     /locus_tag="ACHJ8C_000761"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO5550664.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANL"
     gene            31884..32213
                     /gene="mliC"
                     /locus_tag="ACHJ8C_000762"
     CDS             31884..32213
                     /gene="mliC"
                     /locus_tag="ACHJ8C_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO5550665.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQGVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            32272..32928
                     /gene="pdxH"
                     /locus_tag="ACHJ8C_000763"
     CDS             32272..32928
                     /gene="pdxH"
                     /locus_tag="ACHJ8C_000763"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO5550666.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRHDLPAEPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            33057..34331
                     /gene="tyrS"
                     /locus_tag="ACHJ8C_000764"
     CDS             33057..34331
                     /gene="tyrS"
                     /locus_tag="ACHJ8C_000764"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO5550667.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            34393..35253
                     /gene="pdxY"
                     /locus_tag="ACHJ8C_000765"
     CDS             34393..35253
                     /gene="pdxY"
                     /locus_tag="ACHJ8C_000765"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HFO5550668.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAN
                     PEHYFSATKL"
     gene            complement(35297..35902)
                     /gene="gstA"
                     /locus_tag="ACHJ8C_000766"
     CDS             complement(35297..35902)
                     /gene="gstA"
                     /locus_tag="ACHJ8C_000766"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO5550669.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKSTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(36008..37510)
                     /gene="dtpA"
                     /locus_tag="ACHJ8C_000767"
     CDS             complement(36008..37510)
                     /gene="dtpA"
                     /locus_tag="ACHJ8C_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO5550670.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLTVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLFTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(38121..38756)
                     /gene="nth"
                     /locus_tag="ACHJ8C_000768"
     CDS             complement(38121..38756)
                     /gene="nth"
                     /locus_tag="ACHJ8C_000768"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO5550671.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(38756..39451)
                     /gene="rsxE"
                     /locus_tag="ACHJ8C_000769"
     CDS             complement(38756..39451)
                     /gene="rsxE"
                     /locus_tag="ACHJ8C_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO5550672.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDEKMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(39455..40075)
                     /gene="rsxG"
                     /locus_tag="ACHJ8C_000770"
     CDS             complement(39455..40075)
                     /gene="rsxG"
                     /locus_tag="ACHJ8C_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO5550673.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLETTAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDANWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(40079..41137)
                     /gene="rsxD"
                     /locus_tag="ACHJ8C_000771"
     CDS             complement(40079..41137)
                     /gene="rsxD"
                     /locus_tag="ACHJ8C_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO5550674.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEEIMQYPIYSGMLAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPDTLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(41138..43360)
                     /gene="rsxC"
                     /locus_tag="ACHJ8C_000772"
     CDS             complement(41138..43360)
                     /gene="rsxC"
                     /locus_tag="ACHJ8C_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO5550675.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSVNELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQIDPRKAAVEAAIARAKARKLEQQQANAEPEEQIDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQIDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED
                     "
     gene            complement(43353..43931)
                     /gene="rsxB"
                     /locus_tag="ACHJ8C_000773"
     CDS             complement(43353..43931)
                     /gene="rsxB"
                     /locus_tag="ACHJ8C_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO5550676.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(43931..44512)
                     /gene="rsxA"
                     /locus_tag="ACHJ8C_000774"
     CDS             complement(43931..44512)
                     /gene="rsxA"
                     /locus_tag="ACHJ8C_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO5550677.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(44589..45029)
                     /gene="ydgK"
                     /locus_tag="ACHJ8C_000775"
     CDS             complement(44589..45029)
                     /gene="ydgK"
                     /locus_tag="ACHJ8C_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO5550678.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(45115..45330)
                     /gene="ydgT"
                     /locus_tag="ACHJ8C_000776"
     CDS             complement(45115..45330)
                     /gene="ydgT"
                     /locus_tag="ACHJ8C_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO5550679.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(45603..45728)
                     /gene="blr"
                     /locus_tag="ACHJ8C_000777"
     CDS             complement(45603..45728)
                     /gene="blr"
                     /locus_tag="ACHJ8C_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO5550680.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            45971..47011
                     /gene="ydgJ"
                     /locus_tag="ACHJ8C_000778"
     CDS             45971..47011
                     /gene="ydgJ"
                     /locus_tag="ACHJ8C_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO5550681.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELEALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEIAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(47047..48048)
                     /gene="add"
                     /locus_tag="ACHJ8C_000779"
     CDS             complement(47047..48048)
                     /gene="add"
                     /locus_tag="ACHJ8C_000779"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO5550682.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVADLAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(48152..49324)
                     /gene="malY"
                     /locus_tag="ACHJ8C_000780"
     CDS             complement(48152..49324)
                     /gene="malY"
                     /locus_tag="ACHJ8C_000780"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO5550683.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLIHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKIAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVG"
     gene            complement(49334..50926)
                     /gene="malX"
                     /locus_tag="ACHJ8C_000781"
     CDS             complement(49334..50926)
                     /gene="malX"
                     /locus_tag="ACHJ8C_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO5550684.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYIIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMVGLMRTVQA"
     gene            51101..52129
                     /gene="malI"
                     /locus_tag="ACHJ8C_000782"
     CDS             51101..52129
                     /gene="malI"
                     /locus_tag="ACHJ8C_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO5550685.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRIAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            52241..53008
                     /gene="hdhA"
                     /locus_tag="ACHJ8C_000783"
     CDS             52241..53008
                     /gene="hdhA"
                     /locus_tag="ACHJ8C_000783"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO5550686.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            53237..53827
                     /gene="uidR"
                     /locus_tag="ACHJ8C_000784"
     CDS             53237..53827
                     /gene="uidR"
                     /locus_tag="ACHJ8C_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO5550687.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            54217..56028
                     /gene="uidA"
                     /locus_tag="ACHJ8C_000785"
     CDS             54217..56028
                     /gene="uidA"
                     /locus_tag="ACHJ8C_000785"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO5550688.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVGANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYLLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            56025..>57395
                     /gene="uidB"
                     /locus_tag="ACHJ8C_000786"
     CDS             56025..>57395
                     /gene="uidB"
                     /locus_tag="ACHJ8C_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO5550689.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKMQQQ
                     LISDITN"
BASE COUNT        14572 a        14028 c        14873 g        13922 t
ORIGIN      
        1 cccagaataa tcacattatt ttttctccta accataaagg attagtttat cagcgttaaa
       61 tcttttctca tacactatcg ggttagggaa aatatctggt gttgaggaca gcgtcagcag
      121 cgtattagtt gagtgcctaa tatcaggctg ctgaattgtc agcactgtgt ttgcgaagag
      181 gatgcgaaat aaatgaaccc ggttgatcgt ccactattaa atattggctt aacgcgatta
      241 gaattcttgc gtatatcagg aaaaggcctt gctggtttaa caattgcccc tgctttactg
      301 tcacttttgg gttgcaaaca ggaagatatt gatagcggca cagtagggtt gataaataca
      361 cccaaagggg tgctggtgac ccaacgtgcg cgatgcacgg gttgtcaccg ctgtgaaatc
      421 tcttgtacca acttcaacga tggctcagta gggacattct tctcccgtat caaaatccat
      481 cgcaattatt tctttggcga caacggggtt ggctctggcg gtggcctgta tggcgatctc
      541 aactataccg cggacacctg ccgtcaatgc aaagagccgc aatgcatgaa cgtctgcccg
      601 attggtgcga ttacctggca gcagaaagaa ggctgtatta ccgtcgatca taaacgttgt
      661 attggctgta gcgcctgtac tacggcatgc ccgtggatga tggccaccgt aaataccgaa
      721 agtaaaaaat cctcgaaatg tgtgttatgc ggtgaatgcg caaacgcctg cccaacaggg
      781 gcgttaaaaa ttatcgagtg gaaagatatt actgtgtgaa tcttgcaaag gaaaaaatca
      841 tggctaacgg ttggacaggt aatatattaa gagtcaatct cacgacagga aatattaccc
      901 tcgaagattc cagtaagttt aaaagttttg tcggtggcat gggcttcggc tacaaaatta
      961 tgtatgacga agtaccgcca ggcacgaaat ctttcgatga agcgaataaa ttagtttttg
     1021 ctaccggccc attaactgga tctggtgccc cctgtagttc tcgcgtaaat atcacctcac
     1081 tttctacttt taccaaagga aatttagtcg tcgatgccca tatggggggc ttttttgcag
     1141 cgcaaatgaa attcgctgga tacgacgtca ttattatcga agggaaagcg aaatcaccgg
     1201 tatggctgaa gattaaagat gacaaagtta gccttgaaaa agccgatttc ttatggggaa
     1261 aagggacgcg cgcaacgact gaagaaattt gtcgcttgac cagtccggaa acctgtgtgg
     1321 cggctattgg tcaggctggg gaaaaccttg ttcctctctc tggcatgttg aatagccgta
     1381 accacagcgg cggtgcggga actggcgcaa taatgggttc gaaaaacctg aaagcgattg
     1441 cggttgaagg gacgaaaggg gtcaacattg ccgatcgtca ggagatgaag cgtctcaacg
     1501 attacatgat gactgaactt atcggtgcga ataacaacca tgtcgtgcca agtacgccgc
     1561 aatcgtgggc agagtattca gatcccaagt cacgttggac agcacgtaaa gggctgtttt
     1621 ggggcgcggc tgaaggtggt ccgattgaaa cgggggaaat tccgccaggc aatcagaaca
     1681 cggtcggctt tcgtacctat aaatccgttt ttgacttagg tccggcggca gagaaataca
     1741 cagtaaaaat gagcggctgc cactcttgcc cgatccgttg tatgacccaa atgaatattc
     1801 ctcgggtgaa agagtttggc gtgcccagca caggtggtaa cacttgtgta gcaaactttg
     1861 tccataccac catcttcccg aacgggccga aagattttga agataaagac gatggtcgtg
     1921 tgattggtaa cctggttggt ctgaatttgt tcgatgacta cggcctatgg tgtaactacg
     1981 ggcagttgca tcgcgacttt atttattgtt acagcaaagg tgtgttcaag cgtgttctgc
     2041 ctgctgaaga gtatgcggaa attcgctggg atcaactgga agcgggtgac gttaacttta
     2101 ttaaagattt ttactaccgt ctggcgcatc gcgtgggtga gttgagtcac ctggctgatg
     2161 gttcatatgc catcgcagaa cgctggaatt tgggtgaaga gtactggggc tacgcgaaaa
     2221 ataaactctg gtcgccattt ggctatccgg ttcaccatgc caatgaagcg tcagcgcaag
     2281 ttggttccat tgttaactgt atgttcaacc gtgactgcat gacgcatacc catatcaatt
     2341 ttattggttc cggcttgcca ttgaaattgc agcgtgaagt ggcgaaagag ctttttggtt
     2401 ctgaagatgc ttacgatgaa accaaaaact acacaccgat caatgacgca aaaattaaat
     2461 atgctaagtg gtcgctgttg cgggtctgtt tgcataacgc cgtcaccctg tgcaactggg
     2521 tctggccaat gaccgtttcg ccgctgaaaa gccgtaatta ccggggcgat ctggcacttg
     2581 aagccaaatt cttcaaagcg atcactggcg aagatatgac tcaggaaaaa ttagatttag
     2641 ctgcagagcg tatctttacg ttgcatcgcg cctacacggt aaaactgatg caaaccaaag
     2701 atatgcgtaa tgaacacgat cttatctgtt cctgggtatt cgacaaggat ccgcagatcc
     2761 cggtctttac tgaaggtaca gacaaaatgg atcgtgacga tatgcatgct tcgctcacga
     2821 tgttctacaa agaaatgggc tgggacccac agcttggttg tccgacccgc gaaacattgc
     2881 agcgtctggg gctggaagat attgccgccg atctggcagc acacaatcta ctgcctgcgt
     2941 aaggaatggg agagatgaac catcaggatg aattgccgtt agcggaagtc agcgaaattg
     3001 atgaggcgaa gcggcaatgg ttgcagggga tgcgtcaccc cgttgatacg gtaaccgaac
     3061 cagaaccggc tgaaatcctg gcagagttca ttcgccagca ttctgcggct gggcagctgg
     3121 tggcgcgcgc tgtgtttctc tcgccgccct attcggttgc agaagaggaa ctgtcagttt
     3181 tgctggaaaa cattaagcag aacggagact acgcggatat cgcttgtatg acgggaagcc
     3241 aggatgatta ctattactca acgcaggcca tgagtgaaaa ctacgcggcg atgtctttgc
     3301 aagttgtgga gcaggacatt tgtcgcgcca ttgcacacgc cgttcgtttt gagtgccaga
     3361 cgtatcctcg tccttacaaa gtcgccatgc tgatgcaagc gccatattac ttccaggaag
     3421 cgcagattga agccgcgatc gccacgatgg atgttgcacc tgaatatgcc gatatacggc
     3481 aggtggagtc atcgacggca gtgttgtact tgtttagcga gcgatttatg acctatggca
     3541 aggcgtatgg cttatgcgaa tggttcgagg tagagcagtt tcagaacccc tgaaatttca
     3601 gggggatgtc atggataggg tggacagttg cagccactgc attaggggat aatcagatgt
     3661 ccttcactcg acgcaaattt gttctgggga tgggaacagt aatctttttt actggttctg
     3721 cgtcttctct gttagcgaat acgaggcaag aaaaggaagt tcggtatgcc atgattcatg
     3781 acgagtcacg gtgcaatggc tgtaatatct gcgcccgcgc atgccgtaaa actaaccacg
     3841 tccccgctca gggaagccgt ttatcgatag cacacattcc agtcaccgat aacgacaatg
     3901 gaacgcagta tcactttttt cgccagtcat gtcagcactg cgaagatgct ccttgcatcg
     3961 acgtttgccc gactggggcg tcgtggcgtg atgagcaggg gatcgtgcgg gtagaaaaat
     4021 cgcagtgcat cggctgcagt tattgcattg gcgcttgccc gtaccaggtt cgttacctta
     4081 accctgtcac taaagtggca gataaatgtg atttctgcgc tgagacccga ctggcaaaag
     4141 gctttccgcc catttgcgtc agtgcctgcc cggaacacgc attgatcttt ggtcacgaag
     4201 atagcccaga aattcaggcg tggttgcagg aaaataaata ctatcaatat cagctacctg
     4261 gcgccgggaa accgcatctg tatcgccggt tcggtcaaca tttgattaaa aaggaaaatg
     4321 tatgaacccg tcgcaacatg ctgaacagtt tcagagccag ttggcgaact atgtgccaca
     4381 gttcaccccc caattttggc cggtgtggtt gatcattgcc ggactattgc tggttgggat
     4441 gtggttggtg ctggggctac atgccttgct tcgtgctcgt ggcgtgaaga aatcagccac
     4501 cgatcatggt gagaggattt atctttacag caaagcggtc agattatggc actggtcgaa
     4561 tgcgttactc tttgtattgt tgctggcaag tgggctgata aatcactttg cgctggtggg
     4621 cgcaactgcg gttaaaagtc tggttgcggt gcatgaagtt tgcggatttt tgttactggc
     4681 atgctggctc ggctttgttc tgatcaatgc cgttgggggt aatggtcacc actatcgcat
     4741 tcgtcgtcag gggtggctgg agcgagcggc aaaacaaacg cgattctatt tgtttggcat
     4801 tatgcagggg gaagaacatc ctttcccggc aacaacccag tctaaattta atcccttaca
     4861 gcaggttgcc tatgttggtg tcatgtatgg attgctgccg ttgttactat tgacggggct
     4921 gctgtgtctc tatccgcaag ccgtgggaga tatgtttcct ggcgtaagat actggttatt
     4981 gcaggcgcat tttgctctgg catttataag cctctttttt atcttcggtc atctttatct
     5041 ttgcaccacg gggcgtacgc cacacgaaac ctttaaaagc atggtcgatg gctatcaccg
     5101 gcactaacga atgatcatta cacgagcaga cctgcgagaa tggcgtatcg gagcggtgat
     5161 gtatcgctgg tttctgcgcc atttcccacg tggcggtagt tatgccgata ttcatcatgc
     5221 actgattgag gaaggatata cggactgggc ggagagtctg gttgagtatg cgtggaaaaa
     5281 atggctggcg gatgaaaact tcgcgcatca ggaagtttct tctatgcaaa aactggccac
     5341 agatcccgga gaacgactat tttgcagtca gtttgcccgc agcgacgatc atgctcgcat
     5401 cggctgttgt gaggataacg ccagaatcgc aaccgctggg tatgccgcgc aaattgccag
     5461 tatgggttat agcgtgcgga ttggaagtgt cggctttaac agtcacattg gcagctccgg
     5521 tgcacgcgca cgggttgctg taaccggtaa ttcttcgcgg ataagcagcg ctggagatag
     5581 cagtcggatt gcgaataccg gaatgcgagt acgcgtttgt acattgggtg aacggtgtca
     5641 tgtcgccagt aatggtgacc tggtgcaaat cgccagcttt ggcgcaaacg ccagaattgc
     5701 caactgcggc gacaatgtac acatcattgc cagcggtgaa aattcaacag tagtgagcac
     5761 gggcgtggtc gactcaatta ttctcgggcc agggggatgc gccgcactgg catatcatga
     5821 cggcgaaaga gttcgcttcg ccgtcgccat tgaaggtgaa aacaatattc gagccggagt
     5881 ccgctatcgt cttaatgaac aacatcagtt tgttgagtgc tgatagcttt acctttaatc
     5941 aaacgaaaga cgccgacgcg cacgggatgc aggttttacg accgcccgag ccatcgcctc
     6001 tccaatttct gcacatgccg taagtccctg aacgaaaggc tggtcgtgca tcaaccaggg
     6061 caacgcgccg aacgctacgc gcccacgaat atcttcgggt tgctgtaacg tataatcttc
     6121 gccaacatca gggatttcat cccctgtttt ctgtaattgt tcgcgtagtc cagggaaagg
     6181 aatatctttc actttaagct gacgctgccc gcgggcatca ataaaaacgt caaacgagta
     6241 actgttgtct tccgttttca ggacggtgcg cgactcgtta atttccattt cgtagtcttc
     6301 accgagagcg agaagatgaa tgattcccgc ttcacgtaag gccagtagtc gacgaataga
     6361 ctctgaaggg attgccgcat aattatcgat gaaaacccgg gcaaggcctt tactgaaccg
     6421 tttatggtcg tgttcattta gatacggaac aatttcctgt acggcttcat gcatgcgcag
     6481 aatgacataa cgccagggaa cagtgtgttt ttctcgttta ttgcgttcga cttcctggag
     6541 attttcttct gcccagtcaa atgggtcgcg ttgtttgcgc tcggcaaacc aggcttgagc
     6601 aaaggaatcg acattcaggc tctctaaggc tatgcgttgg ctccagtctg gatcagcaaa
     6661 cttgatttcc tctactatca atctaaatac ccgatccaaa aggcccactt cgcctttttg
     6721 aatctcagca tttaatgcct gatcggtgat gatgtgtaag ggctcgtagg gaatagggca
     6781 atagaaatcg gcttcgggta aaatacctgt gcgcgacatt aacgtaatat ttagcttttc
     6841 acttgcgtta tcgcggtgaa aagtgacgtg ttgtttatca tcttcaatga acgaaccatg
     6901 ctgaatagcc actgccattg ccgcatccag tccgctcaag gatgttccca taatacccac
     6961 gttacacgca tcgacctttg cttccatcag gcctgaccac gggctgggaa aatacgttct
     7021 ggttgcttct tcttcatcag gccagacgtg ccccgttgcg atcaccacta aatcaaacgt
     7081 ctcgctgggt aaatccagat ttgtagcgag catgacgcca gcatttgtaa tttgcagatc
     7141 ggtaacctgg catgactcat aaacagccac tgcaaatttt tgctgtcgtg cctggtctac
     7201 taatcgtaaa aattgatcgc ggaaatattc gcccagcaga attcgcggta aaaactgacg
     7261 atcgtgcaag gtttcttttt taacgccata acgctggaga tggctggctt cttgcttttg
     7321 tagccattcg agatacgtgc aataaatcgg cggtatttca atactggcaa tgtttgccag
     7381 catcagtttt gagttttcat catcactgta tggcatcccg acaccggctt cgtcagcctg
     7441 ctcgaagata gaaatagaaa gtggcgtttg ttgctgtaga agcgagaata aggtgtagat
     7501 ccccgtaggc ccggcaccca caatagcaat tgttttcatt gttacttacc tgtacgcatt
     7561 ctttcgaatt tagtgttatc aggtaagttt ggttgagatt tttagaattt gagaaatttc
     7621 agacagggat gaaaagggca ctaaccgccc ttgattaaaa tctgtcaggc aagttttgcc
     7681 tgattgattt gactcagtga cacattgaca tcgaaaactt tggcgacaac ttcctcaacg
     7741 ccaagatttt tcagtcgggc agtatgtttt tccagatagg taagggcgct tttttcatca
     7801 gagaaaaggt agatcccacc ggcttcgtgg ttcttttcac tttctgtcca aaccttccac
     7861 agaaaaccag gttcctgatt aatcgactca gcaagcggtt taagctgctc agccattgcg
     7921 tcgccaaatg ggccattaaa agcaaaatga agttgtaaca aagttgccac agcgattctc
     7981 cttctgataa cgcacacgaa gacagtgtag atcttttaac gtattggata cagagaagaa
     8041 acagagggac aaagaagaag accagattaa gaaaatctgg tgcgtccgag tggactcgaa
     8101 ccaccgaccc ccaccatgtc aaggtggtgc tctaaccaac tgagctacgg acgcaggatg
     8161 gtgcgttcaa ttggactcga accaacgacc cccaccatgt caaggtggtg ctctaaccaa
     8221 ctgagctatg aacgcaacgt tgtaggtgac aacggggacg aatattagcg gcagagtggg
     8281 aaggtggcaa gaggcaaaac gtaattttct gcggtatttc gaccgtttgc agagctttta
     8341 agcaaattgg ctatattttg ttgatttgca agggtgattt ttattcagga tcgcatttac
     8401 atctgacaca acccaataat ccgtgtatca gagtttaaac agagttgttt gccaccatct
     8461 caaatctgtc gtggataaat ccttcgtagc ctttacactt aggtagcaat aaaggcacac
     8521 agatgcagca aaggaataaa tcatgggatc tgatgcgaaa aacttgatga gcgacgggaa
     8581 cgtgcaaatt gttaagaccg gcgaggttat tggcgcgacg caacttactg aaggcgagtt
     8641 gattgttgaa gctggcggaa gagccgaaaa taccgtggtc acgggggctg gctggttgaa
     8701 agtggcaacc ggtgggatag ccaaatgcgc acagtacggt aacaatggca cgctatcggt
     8761 cagcgacggt gccattgcca cagatattgt tcagtccgag ggaggcgcaa ttagtctctc
     8821 tactctcgct acggttaatg gccgccatcc cgaaggtgaa ttcagcgttg ataaaggtta
     8881 tgcctgcggt ttgttgctgg aaaacggcgg taacctgcgt gtactggaag gccatcgcgc
     8941 ggaaaaaatc attctcgatc aagagggcgg cctgttggtc aatgggacaa cctcagcggt
     9001 cgtggtagat gaaggtggtg aattgttggt gtatccaggt ggggaagcct gcaattgtga
     9061 gattaatcag ggcggcgttt ttatgctggc ggggaaagcc agtgatacgt tgcttgctgg
     9121 tggcaccatg aataatctcg gtggtgaaga ctctgacact attgttgaga atggagccat
     9181 ctatcgtctg ggtacggatg gtcttcagct ctacagttcc ggtaagacgc aaaacctgtc
     9241 cgttaatgtg ggggggcggg ctgaagtgca tgccggtacg ctggaaaatg cggtaataca
     9301 aggtgggaca gtgatcctgt tgtcacccac cagcgcggac gaaaattttg tcgtagagga
     9361 agatcgcgca ccggttgaac tgaccggtag tgttgcatta ctggacggcg cttcaatgat
     9421 tattggctat ggcgcagatc tgcaacaatc aacgattact gtacagcagg gcggtgtgtt
     9481 gattctcgac ggcagtacgg taaaaggtga cagtgtcact ttcagtgttg gtaacatcaa
     9541 tctcaatggc ggaaaactgt ggctgatcac tgatgcggca acgcaggtgc aactgaaagt
     9601 gaaacgcctg cgtggagagg gagccatttg cctgcaaacc agtgcgaaag aaatttcacc
     9661 tgacttcatc aatgtgaaag gggaagttac cggtgatata cacgttgaga taacagatgc
     9721 cagtcggcaa actctgtgta actctctgaa attacagcca gaccaagatg gcatcggcgc
     9781 aacgctccag cctgcgtaaa aacacaggcg caacccgtga aggttgcgcc ttgtctttat
     9841 tagcgggatg ctcgttgcag aatgatggct gacggcagac gttgcaggaa ccgcatacgc
     9901 agcatcatca taatggctgc cgacgtcagg ccaataataa agcctatcca gaagcctgct
     9961 ggccccatag gttcaacgac cagatcggtc agtgccagaa tatagccgct tggcaagccc
    10021 agcacccagt aagccgtaaa ggtgatataa aaaatggaac gcgtatcttt ataaccacgc
    10081 aaaattccac tgccaatcac ctggatagag tcagaaatct gatataccgc cgccagcaac
    10141 atcaaatgcg cagccagcgt tacaacctcg ggattgtcgt tgtacaacag ggcgatttgc
    10201 tctcgcagtg aaaccgtgaa tatggccgtc agggttgcca tacagacacc caccataagc
    10261 ccggtccgcg cagcggtttg cgcatccagc gttgagcctt gacccagacg ataacctacg
    10321 cggatggtta ccgctgccgc cagcgacatg ggaagcacga acatcagtga actaaagttc
    10381 agggcaatct gatgtcctgc gacatcaaca ataccgagcg gagacactaa cagagccacg
    10441 acggcaaaca gcgttacttc aaagaacagt gccagcgcaa ttggcaaacc gagttgaatc
    10501 agtcgtttca taaccgcagg atcgggcttc gcggtgcctt tttcgttacg aatatcgcgc
    10561 atcgagcggg cgcgtttaat gtaagaaacc atggcaagga acatgaccca atacaccgcc
    10621 gcagtagcca cgccacaacc aactccaccg agctcaggca taccgaaatg accataaata
    10681 aagatatagt taaccggaat gttcaccagc aggccgataa agcccatcac catacccggc
    10741 ttggtttttg ccagaccttc acactggtta cgggcaacct ggaagaaaag atagcccggc
    10801 gcgccccata gcaacgcacg cagataaccc acggctttgt ccgccagagc cggatcgatg
    10861 ttttccatgg agcggataat gtagcctgca ttccacagca ccagcataat gagaacggaa
    10921 acaaaacctg ccagccagaa accttgtcgc acctgatgcg caatgcgctc acgtcgaccg
    10981 gaaccattta attgcgcgat aaccggcgtt aatgccagca gcagtccgtg accaaagagg
    11041 atcgccggaa gccagataga agttccgata gcgaccgccg ccatgtcggt ggcactatag
    11101 ccgcccgcca tcacggtatc gacaaaaccc atcgcagttt gggcgatttg cgcgagaatc
    11161 accgggattg ctaatgctaa taacagacgc gcttcactga tatacttctg cacgtgaaca
    11221 ccttttattt gtagttatat gagagactaa aaagccgcct gaatgggcag caaaaagaag
    11281 aagcagggga aattcagtct attgtagcga ggatttatta attctccagt gaaaaaatag
    11341 ccattcttac cccttcactg gtaagggcgt tttccacctg ctattgtgct gaacagaatg
    11401 tcttaactga tttcaggagt tgtaagtatg tttacgggga ttgtacaggg caccgcaaaa
    11461 ctggtgtcga ttgacgaaaa accaaatttt cgcacgcatg tggtggagtt acccgaccac
    11521 atgctggacg gtctggaaac cggtgcttcc gtggcgcata acggttgttg cctgaccgtg
    11581 acggaaatta acggcaacca tgtcagtttt gatctgatga aagaaacgtt acgcattact
    11641 aatcttggcg atttaaaagt gggggattgg gtaaacgttg agcgtgcggc gaaattcagc
    11701 gatgaaattg gcggacactt aatgtcaggt catattatga ccaccgctga agtggcgaaa
    11761 atattaacct cggaaaataa tcgtcagatc tggtttaaag tccaggatag tcagttgatg
    11821 aaatatatcc tgtacaaagg atttattggc atcgacggta ttagcctgac cgtcggcgaa
    11881 gtcacgccaa cgcgtttttg cgtccattta attccggaaa cactggaacg cacgactctt
    11941 gggaagaaaa aactcggcgc acgcgtcaac attgaaatcg atccacaaac tcaggcagtg
    12001 gtagatacgg tagaacgtgt gctggcggca cgagaaaatg ccatgaatca accaggtaca
    12061 gaagcctgat ctgaaatcgc ccccggagag ggggcgatag aatagccttt agcgagcgac
    12121 tcgaaggccg ttttcaacac cgcgtgagaa cacgacctgc cagagctgaa tatcacgggc
    12181 gcggaaagca cctgcacagg cattcagata ataggtaaac atccgtttaa agcgttcact
    12241 gtagttatcc gcaatttctg gccatgcggc gaggaatcgt tcataccacg ccatcaacgt
    12301 agtatcgtaa tcagcaccga agttatgcca gtcttccatc acaaagtggg gttcgctgga
    12361 ctgagcaatc tggcgtacag agggcaggca accgttcgga aaaatatatt tattaatcca
    12421 gggatcaaca ttcagatcgg tttttttcga accgatggta tggagcagga atataccttc
    12481 cggtttcaaa ttacgatcca ccaccgcaaa ataggtatcg taatttttcg gtccgacgtg
    12541 ttcgaacatc cccacagaaa caatacgatc aaactggtcg ttcaggtcac gataatcttg
    12601 cagcaaaatg gtgacatcca ggccttcaca gcgttcctga gccatttttt gctgttcggc
    12661 agaaatggtg acgccgacca cgcttacgtc ataattagac gccatgtagt gcgccagtcc
    12721 accccagcca cagccaatat ccagtacgcg catccctggt tttaactgca atttttcaca
    12781 aatcattttg agcttcgcct gctgggcaga ttccagatta tcggcatctt tccagtaagc
    12841 acaggaatat tgcatgaagg gatcaagcat gcggctgaac aagtcattac ccaaatcgta
    12901 atgctctttg ccgactatcc aggcacgttt tttactctgc agattgaaga gacgagcgcc
    12961 ggcaatacgc agcgtgtctt tgaaatgatg gggaagttgg ttctcgagac ctgcgcgtaa
    13021 gactttgcta aaaaacatat ccagtcggtc acattcccac cagccatcca tataactttc
    13081 gcctaacccc aacgatcctt cctgcagaac gcgtttaaaa aaatcggggt ttttgacacg
    13141 aatatccgcc ggggcagaac cgttaatggc tataccggca cggctaagta attcgttggc
    13201 gatacggtac cagttgtcat ccggtacact gacttcttct atacacgatg aactcatagt
    13261 ttctccatca ctggtcggtg atcagaacct taaaacagcg tagacgcttt ttgggctttg
    13321 tgagaaatct cgcagaaaaa atcgcgagtc tataatccga cgtagaacag agggaaagga
    13381 ggaacccttg ccgaaatgac catccatagt gagtcgggaa cgatcctgtc cccgtaataa
    13441 cataatattt atcgtagttt taacaactcg aaattagtat aggccgctaa tttcaatcat
    13501 acaacaaaat tttgttagca gcctaagtat aagtatatcg atatagatca gtgtgattcg
    13561 ctatgagcga cttcggcatt gccatgattc tggcagccaa cttcttcaca acgttgcatc
    13621 atgtaaccca gagcgaccag cactactgtt gataacatca cgctggtggt ggtgagcaat
    13681 ggcgtactga tactgataag ccaggaaact accagacttg cgaggaagca cagacccagt
    13741 tgcagagtgt tctgcaacgc tgcggcgcga ccggttgcat gtgggaaggg acgcagcgcc
    13801 tgggcaacaa caatcgggta gatcgcgccg ttggcaatcg ccatcacaca gaatgggatc
    13861 aggatttcga ccagcgacac atggctaatg aagcccgcag cccaggtcgc aatgacgctg
    13921 acagcaaaca gtaccagcaa ccacggtaat aactgcttgc cttgccattt ctgcagcgcg
    13981 gcgcgacagc cataaccacc aatcagaaac gcgatagttt gcgggacata acttaaacca
    14041 ataactgccg gactgtaacc catttcacta aggatgaacg gtgaaccggt cagccatgca
    14101 aaaaaactgg ctgaacaggc cgcgtatatc agcacgttgc cgcgataggt tttagaacgt
    14161 agcaggtcgg taaaggtcag accatcctga ctattgttac gggccttcgt cgtgggtttg
    14221 agccagaaaa taggcagaat cagcaccacg gtaatggcaa acagggtggc gaaaatcgcc
    14281 tgccaggaga aatggaccag cagccagctt cctaacagag gagccagtgc cggagacaga
    14341 cccaccagcg gcatgatggt cgcaaaaata cggttaactt tctgtgaagg ataataatct
    14401 gtcactaatg cttgccagat aaccgccgcg gcgcagaccc ccacagcctg tacaaaacgc
    14461 aatatcagca gcgtggcggc gttttctacc cacagcatcc ccagactacc taacgcaaaa
    14521 attgtcaggc cgatgaataa caccggttta cgaccataac ggtcggagag tggcccccac
    14581 agaagctggg ctgcggcaaa accggcaagg aacagactaa ggctggcact gacagcagac
    14641 gcaggcgttt gcaggtcggc ctgtatggcg gcgaaagcag gcagatacat atcggttgcc
    14701 agaaaaccga gtacgctcaa acccgccagc cagactaaaa atcttttccc aggttgcatg
    14761 tatattctct aaatctctca attaaacacc gccgcaaagt gtagggagtg aaattagtgt
    14821 tgtgaaacgg taatatttga tgattggttt caaaaatttt gcaggcagac tatgtggtca
    14881 gaatattctc tggaagttgt tgatgcggta gcgcgtaatg gtagttttag cgccgcagcc
    14941 caggagttgc atcgcgtccc ttctgcggtc agctataccg tgcgtcagct ggaagagtgg
    15001 ctggcggtgc cgctttttga acgacgtcac cgtgatgtgg aactgaccgc tgccggggcg
    15061 tggtttctca aagaaggacg ctctgttgtc aaaaaaatgc agatcacccg ccagcaatgt
    15121 cagcagatag cgaacggctg gcgcggtcag ttagctatcg cagtggataa tattgtcagg
    15181 ccagagcgaa tacggcagat gatcgttgat ttttatcgcc attttgatga tgtcgaactt
    15241 cttgtctttc aggaagtgtt caacggtgtc tgggatgcac tttccgacgg gcgcgtggaa
    15301 ctggcgattg gcgcaacacg cgcgattccg gtaggcggtc gttatgcctt ccgggatatg
    15361 gggatgttaa gctggagttg cgttgttgcc agccaccacc cgctggcgtt gatggatggc
    15421 ccgttcagcg atgatacgtt gcgcaactgg ccgtcgttgg tgcgcgaaga cacctcgcga
    15481 acgctaccta aacgtattac ctggttgctg gataaccaaa aaagacttgt cgtgcctgac
    15541 tgggaatcat cggcaacctg tatctcggca ggattatgca tagggatggt gccaacacat
    15601 ttcgccaaac cgtggcttaa tgaggggaag tgggtagcac tagaactgga aaaccccttc
    15661 ccggattcgg catgttgtct gacatggcag caaaatgata tgtcgcctgc gttaacctgg
    15721 ttgcttgagt atctcggcga tagtgagacg ctgaataagg agtggctgcg ggagccggaa
    15781 gagactcccg caacaggtga ttaacgacga tagtcgcgga acgggccgtc agccacggag
    15841 cggcgttcaa tcaaccgcgg atgcacttca atggactgcg gttcttcacg tttgttgacg
    15901 atacgatcca acagcatgtt gaacgctgtt tcgcccagcg aatcttttgg ctgatggatc
    15961 gtggtcagcg ccggcgtaaa atagcgggcg ttgcgcacgt tatcataacc gatcagcgaa
    16021 acatcctgcg gcacgcgcag acccatttca tcagcagcac aaagtgcgcc cattgccatg
    16081 atatcgccac cacagaagac ggcagtaggg cgatgcgact gcgacaggat ttgctgcatg
    16141 gcgcgataac cggattcagg ttcaaagtca ccctgcacaa tccagctttc cggcactttg
    16201 atcatcgctt cttccatcgc cttcataaaa ccggcaaggc ggcctgcgcc ggtgttacgt
    16261 tccagcgggc cagggataac gccgatttcg cgatgaccgc gttcaatcag ataacgcccg
    16321 gccatgtagc cgccttcgaa cgcgttatca atgaccgcat cggtgaagtc agcttttgct
    16381 tcaccccagt ccatcaccac cattgggata tggcgatact cttccagcat cgccagcaac
    16441 ggctctgggt actcagaaca catcaccagc agaccatcga cgcgtttttg cgccatcatc
    16501 gacagataag cccgctgttt ctcaagattg ttccacgcat tgcccagaat cagggtgtaa
    16561 cctttctgga agcaattttt ttcaaccgct tcaatgatct cggcaaaata ggccgcttcg
    16621 ctgctggtcg ccagcaaacc gatagacttg gtgtggttaa ccttcaggct acgcgccacc
    16681 gcgctagggg agtagtgtaa ttctttaatc gctgcccaca cggcgttgcg cgtttcttca
    16741 gcgacgaaac gtgttttgtt gatcacgtgt gacacagttg tagtggaaac gtttgctcgt
    16801 ttcgctacat cttttattgt tgccattcca tttcactcca gaccctaact tcagctcctg
    16861 caaaatcgca aggtaaacgt ttgcctttac acaccttacg cacaacaaaa gtgttgcggt
    16921 acgccggaaa aacgacacgg agcgtcagga aggggtcaat ggccagtacg caaatagtgt
    16981 aagcgtggaa ttttggccga tcttgacgaa aaggggaagt ggtaaaaaca cttatcatta
    17041 taaatgacgg gagatttttt catcacagtg tgtaaaaatg cgtaatatca ctttttgtgt
    17101 ggtaataaaa aaggagaaaa cttgatgagc accgacctta aattttcact ggtaacaacg
    17161 attatcgtcc tcggtttgat cgtggccgtg ggtttgactg ccgcgctgca ctgatttcct
    17221 ctcgagggag cgcgttctcc ctcgcatctc cttcctgttt gttagcaaaa tggcaaaagt
    17281 gttttctctt tttgttattt tcattttttt gtgattgctg tcatgctttc gccactttaa
    17341 tgaatatcgc gtaatgacgc gaagaggtgg agataaggca tgaaaattaa ctatccgttg
    17401 ctggcgctgg cgattggcgc gtttggtatc gggacaacgg agttctcgcc aatgggcttg
    17461 ttgcccgtca ttgcgcgcgg tgtggatgtc tcgattcccg ctgccggaat gttaatcagt
    17521 gcctatgcag ttggcgtaat ggtgggcgcg ccgctgatga cgcttctact ttctcatcgt
    17581 gcccgccgca gtgcgttgat tttcctgatg gcgattttca cgctcggcaa cgtactttcc
    17641 gccatcgcgc cggattatat gaccctgatg ctttctcgca ttttgaccag cctgaatcac
    17701 ggagcatttt ttggtttggg ttcagtcgtg gccgcaagcg tggtgccaaa acataaacag
    17761 gccagcgcag ttgccactat gtttatgggg ttaaccctgg caagtatcgg tggcgtgccg
    17821 gcggctacct ggttgggtga aaccatcggc tggcggatgt catttctggc aacggcgggg
    17881 ctgggcgtga tttcaatggt aagtctgttc ttctcattac ctaaaggtgg tgcaggggca
    17941 cgacctgaag tgaaaaaaga gctggcggta ttaatgcgtc cgcaggtgct gtctgcattg
    18001 ctgacgacgg tactgggagc tggtgcaatg tttaccctct acacctatat ctctccggta
    18061 ctgcaaagta ttacccacgc aacgccggtg ttcgtcacgg caatgctggt gctgattggt
    18121 gtcggattct ctatcggtaa ctatctcggc ggcaaactgg cagatcgttc agttaacggc
    18181 acgttgaaag gctttttgtt gctgctgatg gtgattatgc tggcaatccc gttcctggcc
    18241 cacaatgagt tcggcgcagc tattagcatg gtggtgtggg gcgctgcaac ctttgcggtc
    18301 gtaccgccgt tacagatgcg cgtgatgcgt gtcgccagtg aagcgcctgg tctgtcttca
    18361 tcagtcaata ttggtgcctt taatcttgga aatgcgctgg gagcagctgc tggtggtgcg
    18421 gtaatttccg ctgggctggg atacagcttt gtgccggtga tgggggcgat tgtcgcggga
    18481 ctggcattat tgctggtgtt tatgtcagcc agaaaacaac ctgaaacagt ttgcgttgcc
    18541 aacagctaat taaacaaaaa cgcagaagga taaccttctg cgttacagtt gctggcaacg
    18601 tgcgatttat cttgccgaat gcgctgttaa cgcctcatcc gtgttggcat ggcgcgcgaa
    18661 gcgcatcagg taatgttgca tttgccatca gttattatgc agcgagattt ttcgctacga
    18721 attcccagtt caccagcgcc cagaagtgct ccagatagcc aggacgtgca ttgcgatagt
    18781 cgatgtaata agcgtgttcc cagacatcaa cggtcagcag cggagtcgca tcggtggtca
    18841 gtggagtacc cgcgttagag gttgaaacga tagccagttt gccatcgctg tttttcacca
    18901 gccaggtcca gccagaacca aagtttttga tcgctgcatc agtaaactga gctttgaaat
    18961 cggcaaagct gccaaaagat gcggcgatag cttcagcgac ttttccagtc ggttcgccac
    19021 ccgcgttcgg tgccaggcag ttccagtaga aagtatggtt ccagacctga gctgcgttgt
    19081 tgaatacgcc accttcagag ctgcgaataa tctcttccag tgatttacct tcaaacgcgg
    19141 tacctttgat caggttgttc aggttagtga cataagtctg atgatgcttg ccgtagtgat
    19201 actcgatggt ttccgcagaa atgtgcggtg ccagagcatc tttagcatat ggtagtgcag
    19261 gtaattcgaa tgacattgct actctccttt attattaatt tgcatacgta caatagcctt
    19321 attgtgcgta tggatagggt agcaaattaa aaggtgagac aaaagatgaa cttaccctgt
    19381 tgccgtaaca acagggtaaa gttttagcga agtgtttttg gggtcattac ccggcgagcg
    19441 ccaacatagt ggcgctgcca gtagtcttca ctgagagaag tgatttgaat ttcctggccc
    19501 gtgcgcggtg actgaataaa tttgccgttg ccgacataca cgccgacatg atcggctgta
    19561 ccgcgtccct gagtacggaa gaagaccagg tcgccgtttt tcagttcact acgttcgatt
    19621 ggcgctgcat cgcgcaggtg atacatttca ttcgccgtac gcggaatacg aattttcacc
    19681 aaatctttat aagcgtaata aaccagaccg ctgcaatcaa aaccggtacg cggtgaactg
    19741 ccaccccaac gatatggctt accaatttgc tgcatcagtt tattcattgc cacttttgtg
    19801 gctttttgca ccttcgcttt gtgcgcgtca gcaagggttc ctgacgtggc atttttgact
    19861 tttacacaat gcgatttata gcccttacgt ttggtacact tttcggtaaa gctggctgag
    19921 gccgttttat tgacggcgtg tttgctgcga gttttggcgt ttttagaagc ggtaatggaa
    19981 gattttttaa cggaagaagc ggttttgctt ttggtcgtac ttttactggc ggcttttttc
    20041 gctgttttct ggctggcttt ctgagtcttt tgggtggttg ccgtgctttt ctttttatct
    20101 gcttttttgg tgatatgagt ggtagcagaa ctctccctgg cttgctttga agcatgggcc
    20161 ataggcgtta aaggcagggt ggtaaaaagt aaagcacaga gcgtgatcga aatacggttt
    20221 atccgcgcca ctgagcgtcc ctctgttaag gcaggtctat catgcctgcg tctgatttac
    20281 aaaacttgat gattctaatg catagaagaa gagaaagtta atagctttta ttgttaaaaa
    20341 atacgtttcg tcggcgagcg caaaaaaatg cagcagtgaa agctaaaaat ggttataaat
    20401 acgccagttt gccgtgcata agggaagaaa cgaatatttg aaatgcaaca attagagcaa
    20461 agggataaaa tagcaaaagc gtgatgaaaa tgttatttcc cgatgccgca ctgacccgct
    20521 acaatgtcag attactgccg taaagaagtt tgaggaagca agacaatgag caccactatc
    20581 gaaaaaatcc aacgccagat tgctgaaaac ccgatcctgc tgtacatgaa aggttcaccg
    20641 aaactgccga gctgcggttt ctctgcccag gcagtccagg cgcttgccgc atgtggcgaa
    20701 cgttttgcct atgttgatat tctgcagaat ccggacattc gtgcggaact gccgaaatat
    20761 gcgaactggc cgaccttccc gcaactgtgg gttgacggcg agctggtggg cggttgtgat
    20821 atcgtgatcg aaatgtatca gcgtggtgaa ctgcagcagc tgatcaaaga aactgccgct
    20881 aaatacaagt ctgaagagcc ggacgcggaa taatccgtcc tgcaatactg acggatgcga
    20941 acgggcatcc gtcagtcctt tctctatccc caatccagcc cttgtggcga acttgaaaat
    21001 cccgcctcac gtaaccgcgt aaacatcggc gtttgccgga ccggtaggtc attcacttct
    21061 gttagcgtaa agcgcagccg tggttcgcga ctggacagct gtcggctgtt taaaggcgcg
    21121 aagaaactag tcgcgggtcg ccggtgaaaa cacgtccggc aggggcgatg aagggtttag
    21181 attatctgcc attaacgcta ttccccgtga atatcatgca tgatgcgtgg aatagcctca
    21241 ctatagatgc ctgaatcatt gcatgtcatc aggcatcgac tcgattacac ctcttcggcg
    21301 gcaggtagcg gccagcctcc cagacgtttc cagcggttga cgatttcaca aaacagcaca
    21361 gcagtgcgtt cggtgtcgta caacgcggag tgcgcctggg tgctgtcgaa gtccatgcca
    21421 gcggtctggc aagcctttga caatacggtt tgtccgagtg ccagcccggc cagtgcagca
    21481 gtgtcaaaag tggcgaaagg gtggaacggg ttacgtttca gtgaggcgcg ttctgcggcg
    21541 gccatcataa agctgtgatc gaaattggcg ttgtgcgcca ccataatggc gcggttacag
    21601 ccgctcgctt taataccttt acgtacaact ttaaaaattt cgtgcagcgc ctcgtactcg
    21661 ctgaccgcgc cgcgatcggg atcgttcgga tcaatgccat tgaaggcaag ggcctctggt
    21721 tgcaaatttg cgccgacaaa tggttcgacg tggaaatgta atgtggtgtc cggcatcagc
    21781 cagccttgtt catccatttt cagggtgatg gcggcaatct caagcagcgc atcggttttg
    21841 gcgttaaatc cggctgtttc aacatcgatc acaacaggat aaaaaccacg aaaacggtcg
    21901 cacagaccgg taagttgagc gttatcggac atctgattct cttaatacgg ataaattgca
    21961 gcgcgcatta tgacaaatat tacttgtcga tgcagtaaag atgcgggcgc gatgagttca
    22021 cgcccggcag gagattagtt gcccagaccg cgaccggcgt ctttctcttc gattaattca
    22081 attttgtaac cgtccggatc ttccacaaat gcgataaccg tagtaccgcc ttttaccgga
    22141 cccgcttcac gggttacgtt acccccgttt tgacggattt tttcgcacgc ttcagcggcg
    22201 ttatctacgc taagcgcgat gtgaccataa gcagtgccga gttcgtattt atccacgccc
    22261 cagttgtagg tcagttcaat cactgcttct tcggtttccg ggccgtaacc aacaaacgcc
    22321 agtgagtatt tgtattccgg gttttcgctg gtacgcagca gtttcatgcc cagcacgttg
    22381 gtataaaaat cgatggagcg ttgcagatcg ccaacgcgca gcatggtatg aagaagacgc
    22441 atctttatat cctcaaaatg gctaacagat tcaaaaagtt gttttagtat agcggcggct
    22501 ttgcgccgct cgcaatgttg gattacagcg tcgggtaatc agtgtaacct tcagcaccgc
    22561 caccataaaa actttcggca cgctgtgggt taagctcagc tttgcgctgc aagcgggcga
    22621 ccagatccgg gttcgcaatc cagtcacgac caaatgctac cgcatcaatt aaccctttgc
    22681 cgatcagcgt ttcagctttt tctactgtgt atgcacctgc gccgataatc ggaccgtgga
    22741 aacgggcgcg tactttttcg cgaaacgcat cagtatatgg ttcacctccc gcccaatctg
    22801 gttctgacat atgaagataa gcaatgccgc gtttacccag ttgttcaatc agatacagtg
    22861 catcggcttc ttcattcggg ccgttatccg tgttctggaa agtaccgatt ggcgaaatgc
    22921 gaatgccaat gcgatcggca ccccattctt caatcccggc atcgaccact tccagcacca
    22981 gacgcgcgcg attttccacg ctgccaccgt actgatcggt acgatggttt gaagaagggg
    23041 aaaggaactg atgcagcaaa taaccgtgag cagagtggag ctctaccaga tcaaaaccgg
    23101 cttcacgcgc gttagcaatg gcctgacgga aatcattgac gatacccgga atctctccca
    23161 gttcaagcgc acgcggcatg gatgtttcaa cacggatcgc ctgaccattt tcatcgcgca
    23221 gagaagtacg tgttcccgcg ctaagtgctg aaggcgctac cggtgcctga ccgccaggtt
    23281 gcaggctggc gtgagaaatg cgtccggtgt gccacagctg tacggccata tgaccatttt
    23341 cagcatgaac gccagcggta atttttttcc atgcggcaat ttgttccggg ctatggatgc
    23401 caggtgcacc tgcgtatcct tttgcctggg cagaaatttg cgtggcttcg ctaataatca
    23461 aaccggcact ggcacgttgg cgatagtatt ccgccatcaa cggggtaggg atgtcacccg
    23521 gttcaatact gcgcagacgc gtcagcggtg ccataaaaat acgatttgcc gccgtgatcg
    23581 cgcccacttt cagtggggaa tacagttttt cagatgacat aaaggctccc ggtcgtctgg
    23641 tgataaaaaa tgcctgctaa acggcaggcg tcgcaataat gttttttaca tgggccagcg
    23701 cgttttccag tggctcgaaa ctgcgcgaaa ttttggcctg caaattcgca ccaagccaca
    23761 gtgcgtaaag cacttgtgcc tgttgcagcg gttcgccaca aaaggttaaa caatggttct
    23821 cacggccatt ttccagcgcc tgagagagca gggcgatcac gccgcgagcc cctttatcca
    23881 ttgcgctgcg catatcttct gacagatcgc acacttcggc agagagtttt actgtcaggc
    23941 aaccactgat ggttccatgt tggcaaaact ggttcagtgt ttgctggtaa taagccagta
    24001 tgcggtcgcg gtagttacct tcgccggatt gcagcaactc agtcagtcgc tggtgatatg
    24061 cggcgtaatg gcgctcaagc atggcaacgc caaacgcttc tttagaacga aagtagtgat
    24121 agaaggaccc tttcggcact tcagcggttt ttaggagttc gcttagcccc atcccggtga
    24181 atccacgttg caggcaaagt tgctcgcccg tcgccaggag atgttcgcga gtatcatgtt
    24241 cggtgtgttt gttcatgcgt tggagtgtag tagaccagtc ggtctagtgc aagcaaagtt
    24301 gctactttgg acattaacag cttcaataat ggtaataatc ttatctgagg agggcgaggt
    24361 ggcggagcag ttagagttct ttcccgtcca gagcccgtgc cggggaattt gccagtctga
    24421 cgaacgcgga ttttgtcgcg gctgttttcg tagccgggat gagcgtttta actggaataa
    24481 gatgagcgat ggcgaaaaac aagaagtgtt gcgcctttgt cggcagcgat taatgcgcaa
    24541 attacgtgta aataagtcag cgccatcaga cgaacctgag caaccatcac tcttttgagc
    24601 ctaatcttgc gtatactcct ctcatatctg ataagaggaa gcaattatgg ttcagcgtat
    24661 tactattgcg ccgcaaggcc cggagttttc ccgttttgtg atgggctact ggcgattgat
    24721 ggactggaat atgtccgccc gccagttggt cagttttatt gaagagcatc tggatctcgg
    24781 cgtgaccacc gtggaccatg ctgatattta tggtggctat cagtgcgaag cggcgtttgg
    24841 cgaggcgctg aaactggcac ctcacctgcg tgaacggatg gaaatcgtca gtaaatgcgg
    24901 tatcgcgacg accgcgtgtg aagaaaacgt cattggccat tacatcactg accgcgatca
    24961 catcattaag agcgccgaac agtcgctaat taatctcgcg accgatcatc tggatctgct
    25021 gttaatccac cgaccagacc cgttaatgga tgccgatgaa gtggcggacg cctttaaaca
    25081 tctgcatcag agcggcaaag tgcgtcattt tggtgtatcg aactttacac ctgcgcaatt
    25141 tgcgctgttg caatcacgcc tgccatttac tcttgccact aatcaggtgg aaatatcccc
    25201 ggtgcatcag ccgttactgc tggatggcac gctcgaccaa ctgcaacaac tgcgtattcg
    25261 tccgatggcg tggtcctgcc ttggtggtgg tcgtctgttt aatgatgatt atttccagcc
    25321 gctgcgtgat gaactggctg tggtggcaga ggagttaaac gcgggctcga ttgaacaggt
    25381 agtttacgcc tgggtactac gtttaccgtc gcagccgctg ccaattatcg gctcaggtaa
    25441 aattgagcgc gtacgggcag ctgtcgaagc agaaacactg aaaatgaccc gtcaacaatg
    25501 gtttcgtatc cgtaaagcgg cactaggtta cgacgtaccg taagcacttt taggaatagc
    25561 cgccgttcaa aaatgtgtca ctggtttaca cttattcagg aatgcacaat gaacggaggt
    25621 cctatgaaac gttttagtct ggccattctg gcgctggttg ttgcgaccgg cgcacaagct
    25681 gccagtgaaa aagtcgagat gaacctcgtc acgtcgcaag gggttgggca gtcaattggt
    25741 agcgtcacca ttactgaaac cgataaaggt ctggagtttt cgcccgatct gaaagcatta
    25801 cctcccggtg aacatggctt ccatattcat gccaaaggaa gctgccagcc agccaccaaa
    25861 gatggcaaag ccagcgccgc agaatccgca ggcgggcatc ttgatccaca aaataccggt
    25921 aaacatgaag ggccagaagg tgccgggcat ttaggcgatc tgcctgcact ggtagtcaat
    25981 aatgacggca aggcgaccga tgccgtcatc gcgcctcgtc tgaaatcact ggatgaagtc
    26041 aaagacaaag cgctgatggt ccacgttggc ggcgataata tgtccgatca acccaaaccg
    26101 ctgggcggtg gcggtgaacg ctatgcctgt ggtgtaatta agtaattaag aagccagcgt
    26161 accttgcggc ggtgcttgct caagttgcga aagcgagcag tacagccgcc agacaattgc
    26221 cgccagttct cgggcggcag gttgatgatg acgggcaagg ctgtcgcaaa ttcgctgtaa
    26281 ttcttcaagt gtggccgcca gtgatttttg ctgaacgcca cgctcactca tcactccccg
    26341 caacagtgaa atacagttat cccgtactcg cgataacgga tcggaacgcg attcccagtc
    26401 gcgcaattgc cagacaacat gagaacagtt cagcaacact acaccccagc gtaataacca
    26461 acggcgagcc agcgcatcct ggctgttact caactgactg acgtgatgat aagtgagcga
    26521 ttcaaattcg ctttcactca gtgttggatg gcggcttagt tgatcgacaa aatcccggcg
    26581 cagcgcgcga atatggcggc ggcttttacg agcatccgat cctggacgca gaatggcgaa
    26641 cgctaaccac gccaacgcga cgccaacaat ttttgccaga ttatcgttaa gaaaatcagc
    26701 aaaatcatac accgggggat tagtgacagc gataaacgaa cccataaaaa caatcagttg
    26761 cccccacagt gcggcaaatt tgggcatctg caatttaaga agctgcattg tcgccagcag
    26821 tggaaagaga aacagtaaaa attgccacag atcgctaatc tgcaccatca ggccaaattt
    26881 gaccacaaag ctgaatagcg aaagtaacac cagcgtgcgc atcagcagtg acaacgactt
    26941 aaacggtgcc gcgacggcgg agtagagtac gcagctaatt gctgccagcg ttaaagcatt
    27001 ggcaccggca tcccattgcg aagcaatact ccatgcgcca atcatcatca acgtacaaaa
    27061 tgtacgcagc ccgctccaca tagcttcggc gttatcggta tgacgagcca ggccggatgt
    27121 ccgtgggagt ctggcacgat catctacacc gctttgcaga cgatgtaatt cctgactact
    27181 ttgcagataa aggcggcaaa aataacgtag tcgctgccag aaggcgacgt gccggtagtc
    27241 ggcgacgttg gtcgggcgta gcggggagat aatacgtgcg acggtgtaaa catctgtttg
    27301 cgaactggcg agcgccgtca gcagctgttc gagaatttct cgcgtggcat ctggcggtga
    27361 gggccagttg agcaacatac gtcgcaggct ggagatgaca ctggtcatac gtaattgctg
    27421 gtggagtagc gcattaagac gcgcgttttg ctggcgaaaa cgatagtggc tccagaaagc
    27481 ctggatacgc agcaaattca tggtcagtat ctgcccaatc accccttcat gtgctgcacg
    27541 aatggcatcg gttgtttcag gccgccagag taaactggca tgttccagta atcgggcgtg
    27601 catgtttttc aatgcggtta aaagggctgt agcatcggaa ctgctcggca ggatcatcat
    27661 catcatgccg ccgcacaaaa taccgactat cacctcgcaa acacgcgcct gagcgatatc
    27721 ccacagctgg ctggcctcag taatattaac catcggaaag gcgatgatgg cagccgtgta
    27781 gcccgccagt tgaaatgcat acgcgacgtt attcgtgaag tgcgcacagg cccaggtaca
    27841 aaagccaagc cacgccgaca tgctcaatag aaaaaaccac ggctcattga gcgtatgtcc
    27901 ggcaagaagc agtgccgcaa tggctccgag caaactgcca gcgatgcgtc cgaggctttt
    27961 gctgatgaca ccgccaacgg tgggaaagct aaccaccgca gccgaggtca tcgcccaata
    28021 gggctcatca agattcaaat aataggcaac cgtcagcgcc agacacatgg caatggtatt
    28081 gcgtaacgca taacgccatt gcgccagcgt ggccctgaac cagggcaaat tgcgcaagga
    28141 ccatgacgat gcgttcatta ttgacctacg gcaatgctac aggtggtgcc cgacaccagc
    28201 gtgacatcgc ttgggacttt atccagcgta aagcggacgg gaacgcgttg ggcgaggcga
    28261 acccagggga cgttaggttt cacatccgga ataaggcttg agtcactttc aacgctctga
    28321 tcataaatcg cccgaccgat actggacgcg tgaccctgta acgttttgtt atcactatag
    28381 agggtaattt gtgctggtgc accttcgcgg atatggcgca atttggtttc ttcaaaataa
    28441 ccaatgacgt aaaacgagtg gctgtcaaca agggcaaaca gcggtttccc ggtgtcagcg
    28501 tagtcgccga tgcgggtagt gagattagtc acccatccac tcaccggggc gcgaatttcc
    28561 gtttgcgcca gttgccattg tgcctgtttt agcgtcgcct gtgcggcatc aacgctggcc
    28621 tgcatcgctt taacattgag gttagcggta tcgagttctt cggcagaaat ataattttgt
    28681 gagagatgac gacgacgatt cgcttcgtta ttggcttttg cgaggtcaga ttgcgctttc
    28741 gccagctgag cctgtgcgtt cagctcggcg atctgaaagg gcgttttatc gatcgtgagc
    28801 agaagatccc ccgcgttaac cagctggttg tcttttatat tcagctcaac aatacgtccc
    28861 gacacctgtg gggtgataga aacctgttct gcgcgtattt tcccgtcgcg tgtccacgga
    28921 gattgcatgt aaaaattcca cagccaccat ccggcaagaa ccgcaactac cgctacaatg
    28981 attgttgaga aatacttaat tgttttaatt gacatagtta ccacgcaatc agtattgcca
    29041 gagcaaggca aacgcaaatc gcaaacagcg ataaatccat taacaaggga tgccagatgt
    29101 caccggcgta gatccagcct cgaagcaggc ggtgtatgac aagccagatg acgaatccaa
    29161 atgcgaaggc tttgaaaaac ggaggaaagt agacggacgc accgaacacc agatcttgta
    29221 agggcaatcc tgttgcattg agcataaact tcacaaagta gattccttta cgacagaata
    29281 tattgcgtta gattaataaa tattctttaa gtgcgaaaaa tttacgcgca atttctgaaa
    29341 aatatgtctg acggtaacca aatgcagcaa tacatttgtt ttagcaatac aattgctgca
    29401 cactattcta aaagccgcat aatatcttag caagctaatt ataaggagat gaaattggaa
    29461 tcgccactag gttctgatct ggcacggttg gtgcgcatat ggcgtgctct gatagaccat
    29521 cgcctgaaac cgctggagtt aacacaaacc cattgggtta cgttacacaa tatccatcag
    29581 ttacctccag accagtcgca aattcaactg gcaaaagcga ttggcatcga gcagccatca
    29641 ctggtccgta cgctggacca actggaagaa aaagggttaa tttcgcgtca aacttgtgcc
    29701 agcgatcgtc gggctaaacg tattaaactg acggaaaagg cagagccact gatcagcgaa
    29761 atggaagccg ttattaacaa aacccgcgcg gaaattttac atggcatctc cgcagaggaa
    29821 ctggaacaac tgattaagct catcgcaaaa cttgagcata atatcattga gttacaggcc
    29881 aaagggtgaa atgaaggggg cgtgtggtca gtaacctgac cacacgcaaa cttatttagc
    29941 gcggggaaac ggtcacctga ctgccattgc tggccagtac gacacgttgg cctggagaga
    30001 aatgagtgtt cccttgtttc tgtaccacca tgatggtgtt gccatcatct ttacgaattt
    30061 ccagctcgac accctgcgtt ttgttcattg cactctgtac gccctgaccg gctacaccac
    30121 ctgcaacagc ccctgctgca gtagccagag aacgcccggt tccgccgcca atagtatttc
    30181 ccaggaaacc accaagtaca gcaccaccaa ttgcgccgat aacgttggaa tcatcaccgc
    30241 cctgaatctg tacctgacgt acattaacga tggtgccata gctgacattc tgtacttgtt
    30301 tcgcttcaga agcggtataa acatcccctg acagggtgtc gttattaaca caaccgacaa
    30361 gagacagacc taccattgaa acaaccaata cgcgtttaat cattgaaaaa tctcctgttc
    30421 accatgaaac gccacgcaag catccctcat ggtcaaagta tatggcatat gtgaatccat
    30481 ggttacatgt tctaccaaat atgagcaaat cataatgaaa gtaggatcaa ccaattataa
    30541 acaaaatatt catgatgatt attgatttga agataatgtt ttactaccaa cggtaaaaat
    30601 tggtcattcc gcatggtatt cacgaaaagt ttatgtttga gtggtgcgca aacgtcagat
    30661 tgaaaaagga tgaactatga aatcgggccg ctttattggc gttatgtcag gcaccagcct
    30721 tgatggtgtt gatgttgtgt tggcgacaat tgatgaacac cgggtcgcac agctggcaag
    30781 tttgagctgg ccgatcccgg tgtctctgaa acaggctgta ctggatattt gccagggcca
    30841 gcagcttaca ctttcgcagt ttggacagct tgatactcaa ctcgggcgac tttttgctga
    30901 tgcggtcaat gccttgctta aggaacagaa cctgcaggcg agagatatcg ttgcgatcgg
    30961 ctgtcacggt caaaccgtct ggcatgaacc gacgggcgtg gcaccacaca ctttacagat
    31021 tggcgataac aatcaaattg tggcgcgcac cggaattacg gttgtcggtg attttcgccg
    31081 tcgcgatatt gccctgggag gacaaggcgc accgctggta cctgcgttcc atcatgcgct
    31141 gctggctcac ccaaccgagc gacgaatggt gctcaatatt ggcggcatcg ccaatctgtc
    31201 actgctcatt cctgggcagc cggtaggggg ctacgatacc ggccctggta acatgctgat
    31261 ggatgcctgg atctggcgtc aggccggtaa accttacgat aaagatgccg agtgggcacg
    31321 ggcgggtaaa gtcattctcc cactgctgca aaatatgctc agcgacccat atttctcgca
    31381 acctgcaccg aaaagtaccg gacgcgaata ctttaactac ggctggctgg agcgccattt
    31441 gcgccatttt ccgggtgttg atccccgaga tgtacaggcg acactggcag aactcaccgc
    31501 cgtgaccatt tctgaacaag ttttgttgag cggtggctgc gaacgattga tggtatgtgg
    31561 tggaggaagt cgtaatccgc tactcatggc gcgtctggcg gcattactgc caggcacaga
    31621 agtcaccacc accgatgccg ttggcattag tggcgatgac atggaagcat tggctttcgc
    31681 ctggcttgcc tggcggacgc tggcgggatt accaggaaat ctgccttccg tcactggcgc
    31741 aagccaggag acggtactgg gggctatttt ccccgctaac ctgtgatatc agagttaact
    31801 gaatttttct taccgcgtct gccgttacac ttggaacgtt agggagggcg tattgccctc
    31861 cagaccagga aagtcttcgg gatatgtcta tgaaaaaact gttactgatc tgtttgccgg
    31921 tccttctcac cggctgtagc gcgttcaatc aactggttga gcgcatgcaa accgatacgc
    31981 tggaatacca gtgtgatgaa aaaccgttga cggtcaaact gaataatccg cgccagggag
    32041 tcagttttgt ttatgataat caactattgc atctcaaaca gggcatttct gcgtctggtg
    32101 cacgttacac cgacggaatc tatgttttct ggtcgaaagg cgaggaagcg actgtctata
    32161 aacgcgaccg catcgtcttg aataactgtc agttacaaaa tccacagcgt tgagattttt
    32221 ccaggggcgg cgcacaatag cgtcacccac tgacaatccg taaagaaaac catgtctgat
    32281 aacgacgaat tgcagcaaat cgcgcatctg cgccgtgaat acaccaaagg cgggttacgc
    32341 cgccacgatc ttcctgccga gccattaacc ctttttgaac gttggctctc tcaggcttgt
    32401 gaagccaaac tggcggaccc taccgcgatg gtggtcgcta ccgtggatga acatggtcag
    32461 ccttatcagc gcatcgtttt actcaaacat tacgacgaaa aaggcatggt gttttacacc
    32521 aacctcggca gccgtaaagc acatcaaatt gaaaataatc cgcgcgttag cctgctgttc
    32581 ccgtggcata cccttgaacg ccaggtgatg gtgatcggta aagcggaacg actttcgact
    32641 ctcgaagtga tgaaatattt tcatagtcgc ccccgtgata gccagattgg tgcatgggtt
    32701 tcgaagcagt ccagtcgcat ttctgcccgc ggtatccttg aaagtaaatt cctggagctg
    32761 aagcagaagt ttcaacaggg cgaagtgcca ttgccgagtt tttggggcgg ttttcgcgtc
    32821 agccttgaac agattgagtt ctggcagggc ggtgagcatc gcctgcatga ccgctttttg
    32881 taccagcgtg aaaatgatgc gtggaagatt gatcgtcttg ccccctgaaa agatgcaaaa
    32941 atcttgcttt aatcgctggt actcctgatt ctggcacttt attctatgtc tctttcgcat
    33001 ctggcgaaaa gtcgtgtacc ggcaaaggtg cagtcgttat atacatggag attttgatgg
    33061 caagcagtaa cttgattaaa caattgcaag agcgggggct ggtagcccag gtgacggacg
    33121 aggaagcgtt agcagagcga ctggcgcaag gcccgatcgc gctctattgc ggcttcgatc
    33181 ctaccgctga cagcttgcat ttggggcatc ttgttccatt gttatgcctg aaacgcttcc
    33241 agcaggcggg ccacaagccg gttgcgctgg taggcggcgc gacgggtctg attggcgacc
    33301 cgagcttcaa agctgctgag cgtaagctga acaccgaaga aactgttcag gaatgggtgg
    33361 acaaaatccg caagcaggtt gccccgttcc tcgatttcga ctgtggagaa aactctgcta
    33421 tcgcggcgaa caactatgac tggttcggca atatgaatgt gctgaccttc ctgcgcgata
    33481 ttggcaaaca cttctccgtt aaccagatga tcaacaaaga agcggttaag cagcgtctca
    33541 accgtgaaga tcaggggatt tcgttcactg agttttccta caacctgttg cagggttatg
    33601 acttcgcctg tctgaacaaa cagtacggtg tggtgctgca aatcggtggt tctgaccagt
    33661 ggggtaacat cacttctggt atcgacctga cccgtcgtct gcatcagaat caggtgtttg
    33721 gcttgaccgt tccgctgatc accaaagcag acggcaccaa atttggtaaa actgaaggcg
    33781 gcgcagtctg gttggatccg aagaaaacca gcccgtacaa attctaccag ttctggatca
    33841 acactgcgga tgccgacgtt taccgcttcc tgaaattctt caccttcatg agcattgaag
    33901 agatcaacgc cctggaagaa gaagataaaa acagcggtaa agcaccgcgc gcccagtatg
    33961 tgcttgcaga acaggtgaca cgtctggtgc atggtgaaga aggtttgcag gcggcaaaac
    34021 gtattaccga atgcctgttc agcggttcct tgagtgcgct gagtgaagcg gacttcgaac
    34081 agctggcgca ggacggcgta ccgatggttg agatggaaaa gggcgcagac ctgatgcagg
    34141 cgctggtcga ttctgaactg caaccttccc gtggtcaggc gcgtaaaact atcgcctcca
    34201 atgccatcac cattaacggt gaaaaacagt ccgatcctga atacttcttt aaagaagaag
    34261 atcgtctgtt tggtcgtttt accttactgc gtcgcggcaa aaagaattac tgtctgattt
    34321 gctggaaata atgcattaag tggaaagggg gagtgagaaa tcactccccc tggtttttat
    34381 acagggaaca tgatgaaaaa tattctcgct atccagtctc acgttgttta tggtcatgcg
    34441 ggtaacagtg cggcagagtt tccgatgcgc cgcctgggcg cgaacgtctg gccgctgaac
    34501 accgttcaat tttctaatca cacccaatac ggcaaatgga ccggttgcgt gatgccgccc
    34561 agccatttaa ccgaaattgt gcaaggcatt gccgccattg ataaattaca cacctgtgat
    34621 gccgtattaa gtggctatct gggatcggcg gagcagggtg aacatatcct cggtatcgtt
    34681 cgtcaggtga aagcagcgaa tccacaggcg aaatattttt gcgatccggt aatgggtcat
    34741 ccggaaaaag gctgtatcgt tgcaccgggt gtcgcagagt ttcatgtgcg gcacggtttg
    34801 cctgccagcg atatcattgc gccgaatctg gttgagctgg aaatactctg tgagcatccg
    34861 gtaaataacg tcgaagaagc ggttctggca gcgcgcgaac tcattgcgca agggccacaa
    34921 attgtgttgg ttaaacacct ggcgcgagct ggctacagcc gtgaccgttt tgaaatgctg
    34981 ctagtcaccg ccgatgaagc ctggcatatc agccgtccgc tggtggattt tggtatgcgc
    35041 cagccggtag gtgttggtga tgtgacgagc ggcttactgc tggtgaaact gcttcagggg
    35101 gcaacgctgc aggaggccct ggaacatgtg accgctgcag tatacgaaat catggtgacc
    35161 acaaaagcaa tgcaggaata tgagctgcaa gtggttgctg ctcaggatcg tattgccaat
    35221 ccagaacatt atttcagcgc aacaaagctc tgaaatatct catttcggcc cacaacgacc
    35281 tgtgggccga tggctattac tttaagcctt ccgctgacag cgcgtcttgt acttccggac
    35341 gttcagccat acgttgcata aatgccgcaa tgtgctctaa cccttccaga ttcagtttca
    35401 ccgcgtatgc ccagcgcaga accgtaaaca gataggcatc agcaattgta aatcgttgcc
    35461 cgcagatcca gtgctcatcc ttcagtgcct cgttcacata ttgcagcttc ttctccagct
    35521 gcgcgcgaac tgtcgatttg tactcttccg gtgtatcagg gcgaaacaga ggtgtgaaac
    35581 ctttatgcag ctcggtggcg atgtaattca gccattcgat ggttttatag cgggaaatac
    35641 tgtttaccgg tgccagcaac tggcggtcgg ggacgctgtc ggcaagatac tgcataatcg
    35701 ctacgccttc cgtcagcaaa gtaccatcgt ccagcagcaa tgcaggtacc tgtcccttag
    35761 ggttaacggc aaagtaatcg tcaccgtttt cgagacgttt tttcattaaa tccacactga
    35821 cgagcgtaaa atcctttccg ctctcacgca gggtgatatg ggaagcgaga gagcaggcgc
    35881 ccggtttgta gaacaatttc atcggtaact ccttttgtct gtggtttaaa gtatggtagt
    35941 gcgttgctga acaaaaaaaa agccgctaac gaaaagttag cggcttgtaa aagagtttcc
    36001 ctgaatttta cgctacggct gctttcgccg ctttgtctgc agcgtcatcc tgcgtcatgc
    36061 ggtgcagttt cggcgcggta aacagcatca gtactgcaat aacggcagta gcgacaccaa
    36121 tctgcaagaa tacgcgacca tagacttcca gtgacatcag cggatcggta acgttatccg
    36181 gcacagccat catacctgca acataaccac caatcaggtt tgcaccggca gtggtcagga
    36241 accagctacc cataatgaag cccatcagac gctgcggaac gagttgtgca accattgcca
    36301 gacccaggcc agagatcatc agttccccga tactctgcag gccatagctc gcgaccagcc
    36361 agcttacaga cacgatacca gcgtcagacg cgaatttcgc acccagcggc agaatcagga
    36421 acgcaccaga acacatcacc atgccgatag caaacttggt tggcattggc agggtatcgc
    36481 ccatcttgtt atagatagcg gccagaatcg ggctaccaat gatgatccag aacgggttca
    36541 gtgcctgata ctgctcaggt tctacggtca ggcccagaat ggtgtgttca acgttacgaa
    36601 tcgcaaagaa gttcagtgac gttggcatct ggctgtacag cacgaagaag ataatggctt
    36661 cgagcatcag gatgaaggca acgatcattt tacgacgcgc agcacctttc atcgcgaagg
    36721 cttctttacc gaagataacc acgataccaa aggcaacaac gcccagcgcc atacgcgcaa
    36781 cttcctgatt gtgcagcagc caggtggcga tagcgatcag tgccacaaca ccaataatgg
    36841 tcagcagcag gttacggtag ttgataggct cgaagtctgg ttttgaaccg tactgtttaa
    36901 cccagcgttg gcagaaggcg aagttaacga tagtgatcag caggcctaca acgctcaacg
    36961 caaacgcaac actccagccg tatttcgcgg ccagccacgg ggtggcgatc atagagaaga
    37021 aagagccgat gttgacggac atgtagtaca tggtgaatgc accgtccaga cgcgggtcgt
    37081 ttttctcata gcatgtagaa agcagagaag acgggttagc tttaaacagg ccgttaccga
    37141 ccgcaatagc cgccataccc atataaacga taccggcgtc gtgaccagac caggcaacca
    37201 gagcataacc aatcgccagc acaatagcgc caagcataat tacgcgttta gtacccagta
    37261 ccttgtcacc taaccagccg ccgatagcga ccagaccata aaccagggca ctaaaggaag
    37321 agaaaagggt gattgaatcc gcttcagaca tacccagttg tttaaccagg taaacagcca
    37381 taattccttg taggccgtaa taaccaaaac gttcccataa ctcaatcgag aagatgagat
    37441 agaacgcctt cggttgttta aaagcgttca aactgacgct ttcagttggt ttttggtttg
    37501 cagtggacac ttttacctct ttttttacat cccatattaa cgggggtgct ttctcgcgtt
    37561 atgtctaaaa aacatacgcg caattgttat agtgggaggg gaaacggcag gtaatgttca
    37621 ctatcttgca ctttcagaca agtcctttgt aataatctgt tacatataac tgaggcgggg
    37681 aattcaacca ttccgggaaa tgttatccag cgttaaattt ttcaaaaaac gtaacggcag
    37741 attttttcac taccctgaga gggtgatgat ggcttaatgg cgatatgttc tactatttgg
    37801 cgtttttcct agtgagatag tccagtttct gaaaaatagc cagtgtaatg ttttgtaggt
    37861 caatatttgt tgcgtgtgta aatttaaatg attttaacct gattcttgca atgtttttac
    37921 attgccacag catcgtgatc ttgatcacga atgaatagaa aatttcgcta ttaaaaaaac
    37981 aattaacctg catttttggt tatctgcaaa cggattacct gatgataacg ctaagcaatg
    38041 gcatcattga cgttaaaaag atgttttaaa agaggataaa gaaaggttat gaatggggta
    38101 atcggtgtta cccctgatct ttagatgtca actttctctt tgtattcaca aagatcttca
    38161 ataatacaag agccacagcg aggcttgcgg gcaatgcagg tataacgccc gtgcaggatc
    38221 aaccaatggt ggcagtcgac tttaaactct gccggaacca ctttcagtag cttttcttct
    38281 acctgttcga cgtttttccc cggcgcaaat tgtgtacgat tacaaacgcg gaaaatgtgc
    38341 gtgtcgacag caatagtcgg ccagccgaat gcagtgttta atacgacgtt ggctgtttta
    38401 cgacctacac caggcagggc ttcaagcgca gcacgatctt ccggaacctc gccattatgt
    38461 tgttccagta atatacggca ggttttgatg atattttctg ctttgctgtt ataaaggcca
    38521 atcgttttga tataggtttt caccccttca acgcccagtt caagcatcgc tgcaggcgta
    38581 ttagccaccg ggtagagttt cgccgtcgcc ttattaacac tgacatcggt cgcctgagcg
    38641 gaaagcagta cggcaatcag caattcaaaa ggcgaactga aattaagctc ggtggtggga
    38701 tgaggattgt tctcacgcag gcgagtgagg atctccaggc gttttgcttt gttcatcaga
    38761 cattccctgt ttcaccgttt ggcaatgcac gttcggcagc tgcttcagta cggcgctttt
    38821 tcattttttc atcaatcagg tattttcctg ccagcatcag tcccaggcca ataaatgcac
    38881 ctggtggcag catcgccagc aggaaagggg agtcggtgtg gaaaatctcc acgcgtaata
    38941 cttttgccca gctacctaac agcgcatctg caccgtcaaa caacgtgcca ttgccgataa
    39001 tttcgcgtag tgaacccagc acgaacatgg cgcaggttgc gcccatacca attgaaaagc
    39061 cgtccagtgc cgaaagcgcc ggaccttttt tggcggcgaa ggcttcagcg cggcccacaa
    39121 cgatacagtt agtgacaatc agcggaataa aaatccctaa tgattgatac aggccaaagg
    39181 cgtaggcgtt gatcagcatc tgtacagcgc tgaccaccga ggcgatgatc atcacgtaaa
    39241 tgggaatgcg gatctcggcg ggtgtccagt gacgcagcgt cgaaatggtc aggttggtta
    39301 gcgtcagtac cagcgtagtc gcaagtccta aacccagagc gttagtggca gtggacgtga
    39361 ccgccaacag aggacaaagg ccgagcagct ggaccagcgc agagttgttt ttccacaacc
    39421 cctgaacaat aacgtcttta atttcgctca cgttttattc tccacaggca ggaagttgag
    39481 aaagttgtgc cggtaacgtc tgagcgtaca atccggcgcg ttttaccgca ttaaccaccg
    39541 cgcggggagt aatcgtcgcg ccggtgaact ggtcgaaatc accaccatct ttcttcaccg
    39601 cccagttcgc atcatccgca ccactgattt ttttacccgc aaaatgggtg atccagtcag
    39661 aaaggcgcag ttcgatttta tcgccaagcc ctggcgtttc gtggtgctct gtcacgcgcg
    39721 tgccaagtac cgtgccgtta aaatcggctc ccaccagcag ctgaatcgca ccggaatagc
    39781 catctggcgc ggttgtttcc agaacggcgg ctaccggttt gtcatcctgt ttggcgatgt
    39841 aaacccgatg ctcaccttta cctaactctg gcgcagtgac cagatagcaa ctctgtgcca
    39901 gcgcattgtt atagcgttcg gctggcagca cctgatcaaa taacgccttt tgttgcagac
    39961 tggcctgttc agcaatcgtc gttttggtca tctggttgat ggccgcagtt aaccctgttg
    40021 aacccgctgc aaatagcgcc aacgtaatgc cgtgttttcg gatagttttc agcatggttt
    40081 accctttgcg atggccgtag acgcgcggac gcgtgtagta atcgatcaga ggaaccgtga
    40141 tgttcgccag caggacggca aaagcaacgc cgtcaggata gccgccgaaa ctgcggatca
    40201 accagactaa taagcccgcc agcgcgccga aaataagacg accacgattg gtcgtagaag
    40261 cggtaaccgg gtcagtcaaa ataaagaatg cgccgagcat ggtcgcacca gacagcagat
    40321 gaatttgcgg tgctgccaga gtatctggtg agaacaacca gcccaacgtt gcgcataacg
    40381 ccagcgttac taagaagctg aggggaatat gccagcgaat cgctttctgc catagcaacc
    40441 acacgccgcc agccagccag gcgagattta cccattgcca accagcgccc gccagcatac
    40501 cgctgtagat cggatattgc ataatctctt caaccgaatg accggcacgg acagaggttt
    40561 taaatgtatc cagcggtgtc gcctgactaa tgccatcaat acctaggcgt agcgtgttca
    40621 tatcaccacc actggcggta tgaccgctga aaataacctg tatggcgtcg ataaaaccag
    40681 ggatgttgac cgcaatttca tgcggcggta accagctggt catctgcacc gggaaggaga
    40741 tcagtaagac cacataacca atcattgccg gattaaacgg gttttgcccc agaccgccat
    40801 acaactgttt agcgatgatc accgcaaaca ccgtacccag tacgaccatc caccatggcg
    40861 cgaggggggg aatacttacc gccagcaata agcctgtcag caatgctgag ttatctttca
    40921 acgttgcggc taccgactgc ttgcgtagtt tgagtacgag agcttcggct aacagagcac
    40981 tgaccgacgc caacaggatc tgaacgagag taccccaacc aaaaaaccac agttgcgctg
    41041 cgattcctgg cacggctgcg agcaacacca acagcataat gcgcgatgtc tggcgctggt
    41101 tatgggtata aggggagcta gctattctga ataccattta gtcctcgttt acaacctttt
    41161 gctgggcggc ttttttggcc tgaacgcggg caatagccgc ggcaactgcg gctttgcgcg
    41221 gatcaacctg ttcttctggt tctgcgttag cctgttgctg ttccagcttg cgcgctttgg
    41281 cgcgggcaat agccgcttcg acggccgctt tgcgcggatc aacctgttct tctggttctg
    41341 cgttagcctg ttgctgttcc agcttgcgtg ctttggcacg ggcaatagcc gcttcgacgg
    41401 cagctttgcg cggatcaatc tgttcttctg gctccgcatt agcctgttgc tgttccagct
    41461 tgcgtgcttt ggcacgggca atagccgctt cgacggcggc tttgcgcgga tcaatctgtt
    41521 cttctggctc cgcattagcc tgttgctgtt ccagcttgcg tgctttggca cgggcaatag
    41581 cagcttcgac ggcggctttg cgcggatcaa tctgttcttc tggctccgca ttagcctgtt
    41641 gctgttccag cttacgtgct ttggcgcggg caatagccgc ttcaactgca gttttacgtg
    41701 gatcagcaac ggttgcagcg tcgttagttt gctgcagttc tgcctgtttc gctctggctt
    41761 gcgctttacg ggcttcccgt gctgcaataa ttgcactgtt atccgggcgt tcgcccgctt
    41821 taatcacaat aggctgtgta gcctgggcct gtttctcttt cacccgcgcc agagcggcag
    41881 caatcgcatc tttatctttg gctgcaggtt gaacggctgc gctcttatgt cgttcaagac
    41941 gagccgcttt ttcgcgctcc agacgagcct ggcgcgcttc gaaacgcgct ttggcttctg
    42001 cggcgcgctt ttcttcctga cgaatagccg caatttcagc tttttcctga cggaaatatt
    42061 gcaccagggg gatattgctc gggcaaaccc acgcgcaagc tccacattca atgcagtcgg
    42121 caatattatg cgtggtggct ttatcgtgtt gctgaccttt gctgaaccag tacagctgtt
    42181 gcggcaaaag atccgcaggg caggcgtcag cacaggcgct acaacggatg caactttgtt
    42241 cttcctgtgg ttcgccaagt tcattgacag agggagccaa aagacagttg gtaatcttta
    42301 cgaccgggac atccagccac ggcaaggtaa agcccattag cgggccgccc ataatcacca
    42361 tttgatcggc agaggggcag aatccggcat cattcaataa atgacgcact ggcgtaccca
    42421 gccgtgccca gacgttgccc gggcgagcga ttgcttcgcc agtcagggtc acgacacgct
    42481 cggtaatcgg ctcgccatca ataacggcac gtttcactgc ataagcggtg ccgacgtttt
    42541 gcattaatac gccgatatcg gatgaacgcc cgccatgtgg aacctgcttc ccggtcagaa
    42601 tgtaggttaa ttgtttagca ccgccagaag gatatttggt gggaatcacc cgcagagaaa
    42661 tatcgtgaga gtctgccagc accgcgcgca gcatggaaat cgcctgcggt ttgttatctt
    42721 caataccgat aagaatttcg cgtggctgca gaatatgcgc aaggatgcgg ataccctcta
    42781 cgacctgagc cgcacaatcc tgcatcaaac ggtcatcggc ggtaatgtac ggctcgcact
    42841 cagcagcgtt gataatcaac gtttcaatct tatctccacc gccctgcaat ttaacgcctg
    42901 tcggaaatcc cgcaccaccc agcccggcaa caccaaactg atgaatgcgc tcgattaact
    42961 cttcgcgact gcgagagcga taatcggccc agccgtcgcg cgggatccag cagtcttcgc
    43021 catcggcatc aataatcaca cttaattcag ctaaagctga aggatgagcc gtagagtggg
    43081 gcgcaatagc cgtaacggta cccgaggtgg gcgcgtgaac cggcagcatt ttgccgcgac
    43141 cacgggtaag cggctggccg cgcaatactt tatcgccgac gctaacgcac aactcacctt
    43201 cagcgccaat atgttgtttc agtggaatga caaaacgctg cgccagtggt acctggcgca
    43261 ggggcgtacc gttggactgg gttttcatct ccggtggatg gatgccgccg ttgaaatccc
    43321 agattttatt ttttctgaat gcagagaata acttaagcat ggtgttccac gggaatgata
    43381 cgcacgggaa tggtgttcag atcccatttc caggagtcag gagtttctgc gaccggttgc
    43441 aacgagatgc agtgcgtcgg gcacggatca acacataaat tgcagcccgt acagagatca
    43501 ctcattaccg tatgcatggc gcgggtagcg ccaacgatgg cgtctaccgg acacgcctga
    43561 atacatttag tgcagccaat acagttattt tcatcaataa ccgccaccat ccgcgctggc
    43621 gttagctctt gcgcttcgcc atccagcggc tgcggctcga cattaagcaa ctcggcaatt
    43681 tttagcatca cagcttcgcc acctggggcg caacggttga ttttttcacc gttacagctg
    43741 atggcttccg cgtaggggcg acagccggga taaccgcact gaccacactg gctctgcggt
    43801 aagatttcgt caattttctc aacgaccgga tcgtcttcca ccgcaaaacg gcgggaggca
    43861 taacccagaa tggcgccaaa cgccaggccc agcaggctca cggcggcaac ggcaatccag
    43921 atagcattca ttacaacttc accaaaccac taaagcccat aaaggccaga gacataagac
    43981 ctgcggtaat taacgcaatg gcattaccgc gaaaaggtgc cgggacatca gccacagcaa
    44041 ggcgttcgcg gatggcggcg aagagcacca tcaccagcga gaaaccgaca gcggcggaaa
    44101 aaccgtacag cgctgactgc aagaaattgt gcccgagatt gatattcagc aacgccacgc
    44161 cgagcacggc acagttggtg gtgataagcg gcaaaaaaat ccccagcaga cggtaaagta
    44221 ccgggctggt tttgcgcacc accatttcgg tgaactgcac aaccacagca atcaccagaa
    44281 taaacgccag ggtgcgcagg taaatcagat taagtgggat caaaatccac gtatcgataa
    44341 gccaggcgca aatagacgcc agcgtcatca caaacgttgt tgccagcccc atgcccatcg
    44401 cggtttccag ctttttggaa acccccataa acggacagag accgagaaac ttgaccagta
    44461 caaagttatt gaccagtaca gttccgacaa acagtaacag gtagtcagtc atttttcggc
    44521 ctgaaataaa aaagccgcct attatccgtt aatccagagc aggcgacaac aggttaactg
    44581 taaggttatt acggattcac aaacgtcgct ttcacccgcg acgagcgttt gaaatagggg
    44641 acgatcaatg cggttgccaa caaagtaaac agcaactggc gaacggctat gccatcttct
    44701 acgggtgaaa aagcgaacgc ttttactgcc agcaatacgg aaataagcag ccagataata
    44761 tagtgtttag gaacacaacg ccgacgttta aagaaagcga tggtcaacca cagtgtgtaa
    44821 taccacatgg cgatagcggt aataaaagaa acgccccata agatttgtgt cgtcagtgct
    44881 tgtccgccga gcgtttgaaa tgtttgcgga gaagataacg cagcggtata cagcaacaac
    44941 gccagcgtcg tactcaacag agcgactaaa agccaggcca acgggccaag caaccagcct
    45001 ccaatacgtt gtggcgttgt ggtggtcata ggttctccag aatagcgacg caaacagaca
    45061 aatctaattt taggggcgtt attataaggc ttttataacc gatcgctact ttctttactg
    45121 gacatagtgc cagacggact tcggtacctg gccgaggtca aacaaacgcc cgccagaaac
    45181 cagctctgcg cgacggtgat cggcagcacg atacatattg atcagttcct gatcgtccgt
    45241 cagggtgtaa ttaagatggt cgtagagttt ttccagactt tcgagtgaac tgattttgcg
    45301 aaattttaat aagtagtcct gaacagtcat aaaaggtcca taaaataatt aataccaaag
    45361 gggtaggtat tattagtgat gataaaattt caccaaaagt gaacgcttgt gttattacaa
    45421 taataagaat gtgctgatgg caaatattca gggatgaaaa actgctctgg aaagagtaac
    45481 gcgctttact atccctgaca atcatcaaca acatcgaaca agataataaa ttcctggttt
    45541 aatatccgac aagtgaaaac atgcacccgg acgggcagca tgtcgctcaa caagtgcaga
    45601 gcttacttgt gttgtaccgt agtactctgt tcaggtggct gatagttgtc aatgtgactc
    45661 gccacgccaa gaagaatgac tgaaacgaca agaacgatcc aacctgttaa ttcaataaga
    45721 cgattcatta cagcccacat ctctgttgat tgatccatta acttcagggg gtaaatgtta
    45781 cttagcaata atagctccag caagattttt actgaggttt ttgcgatatt tagcttttgt
    45841 cgttggaaaa atcgctattt ttttgactta agttaaacaa catcccttat tgctggcagg
    45901 ttattaaact gttgagcgag ggtaaaggat agtgtcaaat agccatcata cttcaatgag
    45961 aggcaatgac atgagcgaca acatccgtgt tgggttgatt gggtatggtt atgcgagcaa
    46021 aaccttccat gcgcccctga ttgcgggcac gcccgggctg gagctggcgg taatctccag
    46081 cagcgatgaa acaaaagtaa aagctgactg gccgacggtt acggttgtct ctgagccgaa
    46141 gcatctgttt aacgatccca acatagacct gattgtcatt cctacaccca acgataccca
    46201 ttttccgtta gccaaagcgg cgcttgaggc gggtaaacat gtggtcgttg ataaaccctt
    46261 taccgtgaca ctgtcacaag cacgagagct ggaagcgctg gcaaaaagcc tggggcgagt
    46321 gctgtctgta ttccataacc gtcgctggga tagcgatttc ctgacgctaa aaggtttact
    46381 cgcggaaggc gtgctgggtg aaattgctta ctttgagtct cattttgacc gcttccgtcc
    46441 gcaggtgcgc gatcgttggc gtgaacaggg cggtcctggc agcggtatct ggtacgattt
    46501 agcaccgcat cttcttgatc aggccattac gctatttggt ttaccggtca gcatgacggt
    46561 tgatttggca cagttacggc ccggagcgca gtcgactgat tatttccacg ccatcttgtc
    46621 ctatccgcag cggcgagtca ttttacacgg caccatgctg gcagccgctg agtcagcacg
    46681 ttatatcgtg catggatccc gaggcagtta tgtgaaatat ggcctcgatc cacaggaaga
    46741 acgtctgaaa aatggcgagc gtctgccgca ggaagactgg ggctacgata tgcgtgatgg
    46801 cgtacttacc cgcgtggaag gtgaggaacg tgtcgaagaa acgctgttga cagtgccggg
    46861 gaattatccg gcttactatg cggctattcg tgatgcgtta aatggcgatg gtgaaaatcc
    46921 ggttccggca agtcaggcaa tccaggtaat ggagttgatt gagctgggca tcgaatccgc
    46981 caaacatcgc gcgactttgt gccttgcgtg aaaaggggat ggtgcaatta gcaccatcca
    47041 tttttgttac ttcgcggcga ctttttctcg cagtgcgcgt ttttcctctg cgctgaggaa
    47101 agccatttcc agaccattaa tctgtgcctg gcggatttgc tcgcgggata acccagcagc
    47161 tggcgcggca acggtatatt cgtgaatgat atccactccc tgcacgcctg gatcgtcagt
    47221 gttaatgctg gcacgaatgc catgctcaag gaacgttttc agcggatgtg cagccagatc
    47281 cgccacggtg ctggtctgaa tattggaggt cagacaggat tcaataccaa tttgttgctc
    47341 ggcgagaaaa tccatcagcg cccgatcttc aatggctttt acgccatgtc caatacgctc
    47401 cgcccccagt tcacgaatcg cctgccagat gctttccggt ccggcagctt cgcctgcatg
    47461 gacggtaata tgccagcccg catcacgcgc gcggttgaaa tgagaaagga acagacttcc
    47521 cgggaaacca agttcatcac cggctaaatc aagtgcggta atctggtcac ggtgggctaa
    47581 aaaggcctcc agctcttgct gacaggcggc ttcgccgaag gtccggctca taataccgat
    47641 aagcttcgcc tgcacaccaa aggtgcggca accttcacgt acgccatcga tcaccgcttc
    47701 gacaacaccc gctacaggca gctgatgtgc cattgccatg tagcctggtg aaaaacgcag
    47761 ctcgacatag tgcaggccgt tacgggctgc atcttcaatg ttttcaaatg ccacgcggcg
    47821 gcaggcatca agagaggcga gaactttaac gccccagtca agtttagtca gaaagctcac
    47881 cagatcgggt tcgttggcaa tgacctgaac gtggggaatc agtgtttcca gggattgtgc
    47941 aggaagcgag atattatact ggcggccaag ttcaagaatg gtctggggac gaatgttgcc
    48001 atcaaggtgg cgatggatat cagttaatgg cagggtggta tcaatcatgg tcgcactctt
    48061 tttctggata taaaagtgcg caccattata aaaacaaagc agggtaaaaa tccacgattg
    48121 cgcaacgatt ttttacgttg cgcaattggg gttaaccaac agcgcggatg gcgttaatta
    48181 atccagccac acctttttcc agtttcgaac gtgggcagcc ggcattgagt cggacaaaac
    48241 cacgaccttc ttcaccgtag gtatatcccg gcatgatcgc gatcttttct tgctcgataa
    48301 gcgctttttg caacgcgttg tcgtcaatat tcaacggacg tagatcaagc caggccagat
    48361 aagtggattg cgggatctgc cagttgagtt caggaaacgc agcgttcatt ttatctgcga
    48421 tatacgtcag gttatctttc agatagacgc gtaaggcatc cagccatggc gcgccttgct
    48481 gataggcggc gatatgagca gttaacgcca gtaccgaagg ggaagaaagc ccatcacgtc
    48541 ctttcagtgc cgataaatag gcatcgcggc tactgctatt ttctataatc ccgtaagcac
    48601 cggtcagggc gggaatattg aaactttttg atcccgacgt tagcaacgcc cagtctccgc
    48661 gcgccacatt actccaggga atatgcggct gctcgcccca aaccatatcc atatggattt
    48721 catcggaaat aacccgcaca ccatgacgct cgcacaggtc agccatgatc tccagctcat
    48781 cgcacgtcca cactttcccg gtaggattct gtgggctaca caggagcata attttacatt
    48841 ctggtttcgc cagcacggct tccagcttgc ccatatcgca aaaccagccg tcagcctgct
    48901 tctctaaagc aacgggcatt actgtgcgct ggttaccttc aatggccttg taaaatgcgt
    48961 cataggcggg tgtgtggatc accacgcctt cacctgtttc agaccactga cgaatcagtt
    49021 ctgaaaccat atagataaca gaagggccat acaccaccga ctgaggatcg atggcggtgt
    49081 aatgctgggt ggaaaaccag tgggcaatag ccgcgagaaa ctcatcgttt ttccagcggc
    49141 tgtagccaaa cacgccgtgt atcaggcgct gattcagcgc ctcgataatg cagggggcag
    49201 tggcaaaatc catgtctgaa atcgtgaacg gtaacaggtc agcagtgccg aaacggtcag
    49261 cgacataatc ccactgcgta caccatgtgc catgacgatc cacgaccttt gaaaaatcga
    49321 acatatctta tccttatgcc tggacagtac gcatcagacc gaccatttca tcttttactg
    49381 actgaacttg tggcccgata acaacctgca ggttatgttg attaagttgt actacgccaa
    49441 ttgcccgatt gtccttcagt gcctgcacat taacaagcga catatctttt acagacaaac
    49501 gcagacgcgt aatgcagtta tcgagactga caatattgtc ggcaccgcct aaggcttcga
    49561 ggattgccgg aacgttgtaa cctgatttac ccggcgcacc ggcaacggct ttttcgattg
    49621 aactggcaac ttcactatcg cgccccgggg ttttgagatt gaagcgggtg atagcgaaac
    49681 ggaagatgat gtagtaaacg acaaaccaga ttgccgccac tactggcacc atgtaccact
    49741 tggttgacag accatgcaaa ataccgaaca ccacaaagtc gatgatattg ccgtcggtat
    49801 taccgatggt gacgccgagc acagacatga cagtgaagcc gaggccggtt aacagcgcgt
    49861 ggatgacata cagaactggc gctacgaaca ggaacaggaa ttccagcggt tcggtcgtgc
    49921 cgccaacgac acaggcaatc aggccagaaa tcagtagacc tttaatttta tggcgatttt
    49981 ccgggcgcgc gcagtgatac atagctaacg ctgcacctgg cagaccgccg agaaacgcag
    50041 gcattttacc ttgcgaaagg aaacgcgtgg cgctttcaga aaaaccgtga gtggttgggc
    50101 aactcaattg cgcctggaag atggtcagtg caccgctgac ggtttgaccg cagacttcct
    50161 gcgtgccgcc tgcgtcggta aaacgaatta atgccaccag aatgtgatgc agaccaaacg
    50221 gcaacagcag acgttcaccg gtaccaaaca gcatcggtcc gaaatctccc gcgctgttga
    50281 tcatatggcc taagccgcta atacccatgg cgaaaatcgg ccagactaat ggaatcacca
    50341 ggccgacaag acccatcact agcgaggaga tgattggtac gaagcgcgta ccgccgaaga
    50401 atgccagcgc atccggcagg cggatattat ggaatcgctc atgcagcatc cagacgataa
    50461 taccggcgat caccgcaccg aggatcccgg tgtcgatcga ctggatccca agaatgctct
    50521 ggatgttatt ggctttcaga accgcggcgt ccgtggttgg cagaatgcct ttattggtca
    50581 accagaagtt taccgcgagg ttcattaccg cataaccgac gaagccagcg aatgccgcta
    50641 cgcctttgtt ttcgcgtgcc agacccagcg ggatggcgat acagaacatg actggcaaga
    50701 aactaaaagc aaacgagcca atcttactca tccaggtaaa gatagcctgc aacacggggt
    50761 tgcccaggac cgggatcagg gtgatgacat catggctgct aagagaacta ccaatgccga
    50821 gcataatgcc gcagaacgac aataatgcca cgggtaacat gaaggttttg cctaactgct
    50881 ggaagaactc ccacagcgtg actttcggtg ctgttttcgc cgtcataaaa cgactcctcg
    50941 tagagaacaa tctggattca ataaattgcg cgagaagata aaacgtttta tcaaatttta
    51001 gtgaggcata aatcacatta cacaacgata atagcgggta taagataaat aaaaggtaaa
    51061 acgttttatc tgtcacataa tcagggagta ggtcatctgc atggctaccg ccaaaaaaat
    51121 aaccattcat gatgttgcgc tggctgcggg cgtgtcggta agtaccgttt cgctggtgct
    51181 tagtggcaaa gggcgaatct ctaccgccac aggagaacgc gttaacgccg ccattgaaga
    51241 gctgggattt gtgcgcaatc gccaggcgtc ggcgctgcgc ggcgggcaaa gcggcgtcat
    51301 tggtttgatc gtccgtgatt tatctgcgcc gttttacgcc gaattgacgg ccggattgac
    51361 ggaagctctg gaagcgcagg gacggatggt ttttttgctt cacggcggta aagacggcga
    51421 gcagctggca cagcggtttt cactattact gaatcagggt gtcgatggtg tggtcattgc
    51481 cggggctgca ggaagcagcg atgacctgcg acggatagca gaagaaaaag ctatcccggt
    51541 gattttcgct tcccgtgcca gttatcttga tgacgttgat acggttcgcc cggacaacat
    51601 gcaggctgca cagttgttga cggagcatct cattcgcaat gggcatcagc gtatcgcctg
    51661 gctgggaggg caaagttcct cattaacccg ggcagaacgg gtggggggct attgtgcgac
    51721 tctactaaaa tttggcctgc cgtttcacag cgattgggtg ctggagtgca cttccagcca
    51781 gaagcaagcc gcggaagcta tcacggcgct tttacgtcat aacccgacca tcagcgccgt
    51841 ggtttgctat aacgaaacta ttgcgatggg ggcatggttt ggtttgctga aagcaggaag
    51901 gcaaagcggg gaaagcggag tcgatcgtta ctttgagcaa caggtttcgc tggcggcatt
    51961 taccgatgca acgccaacca cacttgatga tatacccgtt acctgggcca gcacgccagc
    52021 gcgggaactt ggtaccacac ttgcggatcg catgatgcaa aaaatcaccc atgaagagac
    52081 gcattcacgc aatcttatta ttcccgcccg gctcattgcg gcgaaataat cctctcttta
    52141 tctgctatac ctggtagtgt cctttcctca aggttaatga taaaaaatgt ggcacacagg
    52201 cggtgccttt tatttttact gaagttcaca ggaggtttat gtgtttaatt ctgacaacct
    52261 gagactcgac ggaaaatgcg ccatcattac aggtgcgggt gcaggtattg gtaaagaaat
    52321 cgccattaca ttcgcaacag ctggcgcatc tgtggtggtc agtgatatta acgccgatgc
    52381 agctaatcat gttgtagatg aaattcaaca actgggtggt caggcatttg cctgccgctg
    52441 cgatattact tccgaacagg aactctctgc actggcagac tttgccgtca gtaagctggg
    52501 taaagttgat attctggtta acaacgcagg tggcggtggg cctaaaccgt ttgatatgcc
    52561 aatggcagat tttcgccgcg cttatgaact gaatgtattt tcttttttcc atctgtcaca
    52621 acttgttgcg ccagaaatgg aaaaaaatgg cggtggcgtt attctgacca ttacttctat
    52681 ggcggcagaa aataaaaata taaacatgac ctcctatgca tcatctaaag ctgcggccag
    52741 tcatctggtc agaaatatgg cgtttgacct gggtgaaaaa aatattcggg tgaatggcat
    52801 tgcaccgggg gcaatattaa ccgatgccct gaaatccgtt attacaccag agattgaaca
    52861 gaaaatgttg caacacacac caatcagacg tctgggccaa ccgcaagata ttgctaacgc
    52921 ggcgctgttc ctttgctcgc ctgcagccag ctgggtaagc ggacaaattc tcaccgtctc
    52981 cggtggtggg gtacaggaac tcaattaata cactaacgaa ccggtaaaca gccgtatgtg
    53041 ttgtttaccg ggatagcgtg tgcaactcgt caacgtctct gctaaacaac tggcagccaa
    53101 atcacggcta ttggttaacc aatagccgga taaaaagctt acgaattgag tgtgccttcg
    53161 cactattcaa cagcaatgat aggcgctcac ctgacaacgc ggtaaactag ttattcatgc
    53221 taactataat ggtttaatga tggataacat gcagactgaa gcacaaccga cacggacccg
    53281 gatcctcaat gctgccagag agattttttc agaaaatgga tttcacagtg cctcgatgaa
    53341 agccatctgt aaatcctgcg ccattagtcc cgggacgctc tatcaccatt tcatctccaa
    53401 agaagccttg attcaggcga ttatcttaca ggaccaggag agggcgctgg cccgtttccg
    53461 ggaaccgatt gaagggattc atttcgttga ctatatggtc gagtctattg tctctctcac
    53521 ccatgaagcc tttgggcaac gagcgctggt ggtcgaaatt atggcggaag ggatgcgtaa
    53581 cccacaggtc gccgccatgc ttaaaaataa gcatatgacg atcacggaat ttgttgccca
    53641 gcggatgcgt gatgcccagc aaaaaggcga gataagccca gacatcaaca cggcaatgac
    53701 ttcacgttta ctgctggatc tgacctacgg tgtactggcc gatatcgaag cggaagacct
    53761 ggctcgtgaa gcgtcgtttg ctcagggttt acgcgcgatg attggcggta tcttaaccgc
    53821 atcctgatcc tctctctctt tccgcgggct ggtgacaact gtgcccgcgt ttcatatcgt
    53881 aatttctctg tgcaaaaatc atcctcccag ggatcggaga atcccaccta aatattcatc
    53941 gtagcaatat atcataatgg tttatcgctc ccaatatgtt cgaacgaacg ttcgattgct
    54001 tattttgtgg cttctgtcaa cgctgtttta aagattaatg cgatcaatat cacgctgtgg
    54061 gtattgcagt ttttggtttt ttgatcgcgg tgtcagttct ttttatttcc atttctcttc
    54121 catgggtttc tcacagataa ctgtgtgcaa cacagaattg gttaactaat cagattaaag
    54181 gttgaccagt atttttatct taatgaggag tcccttatgt tacgtcctgt agaaacccca
    54241 acccgtgaaa tcaaaaaact cgacggcctg tgggcattca gtctggatcg cgaaaactgt
    54301 ggaattgatc agcgttggtg ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgccg
    54361 ggcagtttta acgatcagtt cgccgatgca gatattcgta attatgtggg caacgtctgg
    54421 tatcagcgcg aagtctttat accgaaaggt tgggcgggcc agcgtattgt actgcgtttc
    54481 gatgcggtca ctcattacgg caaagtgtgg gtaaataatc aggaagtgat ggagcatcag
    54541 ggcggctata cgccatttga agccgatgtc actccgtatg ttattgccgg gaaaagtgta
    54601 cgtatcactg tttgtgtgaa caacgaactg aactggcaga ctatcccgcc gggaatggtg
    54661 attaccgacg aaaacggcaa gaaaaagcag tcttactttc atgatttctt taactacgcc
    54721 gggatccatc gcagcgtaat gctctacacc acgccgaata cctgggtgga cgatatcacc
    54781 gtggtgacgc atgtcgcgca agactgtaac cacgcatctg ttgactggca ggtgggggca
    54841 aatggtgatg tcagcgttga actgcgtgat gcggatcaac aggtggttgc aactggacaa
    54901 ggcaccagcg ggactttgca agtagtgaat ccgcacctct ggcaaccggg tgaaggttat
    54961 ctctatgaac tgtgcgtcac agccaaaagc cagacagagt gtgatatcta cctgctgcgc
    55021 gtcggtatcc ggtcagtggc agtgaagggc gaacagttcc tgatcaacca caaaccgttc
    55081 tactttactg gctttggtcg tcatgaagat gcggatttgc gcggcaaagg attcgataac
    55141 gtgctgatgg tgcacgatca cgcattaatg gactggattg gggccaactc ctaccgtacc
    55201 tcgcattacc cttacgctga agagatgctc gactgggcag atgaacatgg aatcgtggtg
    55261 attgatgaaa ctgcagctgt cggctttaac ctctctttag gcattggttt cgaagcgggc
    55321 aacaagccga aagaactgta cagcgaagag gcagtcaacg gggaaaccca gcaggcgcac
    55381 ttacaggcga ttaaagagct gattgcgcgt gacaaaaacc acccaagcgt ggtgatgtgg
    55441 agtattgcca acgaaccgga tacccgtccg caaggtgcac gggaatattt cgcgccactg
    55501 gcggaagcaa cgcgtaaact cgacccgacg cgtccgatca cctgtgtcaa tgtaatgttc
    55561 tgcgacgctc acaccgatac catcagcgat ctctttgatg tgctgtgcct gaaccgttat
    55621 tacggttggt atgtccaaag cggcgatttg gaaacggcag agaaggttct ggaaaaagaa
    55681 cttctggcct ggcaggagaa actgcatcag ccgattatca tcaccgaata cggcgtggat
    55741 acgttagccg gcctgcactc aatgtacacc gacatgtgga gtgaagagta tcagtgcgcc
    55801 tggctggata tgtatcaccg cgtctttgat cgcgtcagcg ctgtcgtcgg tgagcaggta
    55861 tggaatttcg ccgattttgc gacctcgcaa ggcatattgc gcgttggcgg taacaagaaa
    55921 gggatcttca ctcgcgaccg caaaccgaag tctgcggctt ttctgctgca aaaacgctgg
    55981 accggcatga acttcggtga aaaaccgcag cagggaggca aacaatgaat caacaactct
    56041 cctggcgcac catcgtcggc tacagcctcg gtgacgtcgc caataatttc gccttcgcta
    56101 tgggggcgct cttcctgttg agttactaca ccgacgtcgc tggcgtcggt gccgctgcgg
    56161 cgggcaccat gctgttactg gtgcgggtat tcgatgcctt cgccgatgtc tttgccggac
    56221 gagtggtgga cagtgtgaat acccgatggg gaaaattccg cccgttttta ctcttcggta
    56281 ctgcgccgtt aatgatcttc agcgtgctgg tattctgggt gccgaccgac tggagccata
    56341 gcagcaaagt tgtgtatgca tatttgacct acatgggact cgggctttgc tacagcctgg
    56401 tgaatattcc ttatggttca cttgctaccg cgatgaccca acaaccacaa tcccgcgccc
    56461 gtctgggcgc ggctcgcggg attgccgcgt cattgacctt tgtctgcctg gcatttctga
    56521 taggaccgag cattaagaac tccagcccgg aagagatggt gtcggtatat catttctgga
    56581 caattgtgct ggcgattgcc ggaatggtgc tttacttcat ctgcttcaaa tcgacgcgtg
    56641 agaatgtggt acgtatcgtg gcgcagccgt cattgaagat cagtctgcaa accctgaaac
    56701 ggaatcgccc gctgtttatg ttgtgcatcg gtgcgctgtg tgtgctgatt tcgaccttcg
    56761 cggtcagcgc ctcgtcgttg ttctacgtgc gctatgtgtt aaatgatacc gggctgttca
    56821 ctgtgctggt actggtgcaa aacctggtcg gtactgtggc atctgcaccg ctggtgccgg
    56881 ggatggtcgc gaggatcggt aaaaagaata ccttcctgat tggcgctttg ctgggaacct
    56941 gcggttatct gctgttcttc tgggtttccg tctggtcact gccggtggcg ttggttgcgt
    57001 tggccatcgc ttcaattggt cagggcgtta ccatgaccgt gatgtgggca ctggaagctg
    57061 atactgtaga atacggtgaa tacctgaccg gcgtgcgtat agaagggctc acctattcac
    57121 tattctcatt tacccgtaaa tgcggtcagg caatcggagg ttcaattcct gcctttattt
    57181 tgggattaag cggatatatt gccaatcagg tgcaaacgcc ggaagttatt atgggcatcc
    57241 gcacatcaat tgccttagta ccttgcggat ttatgctgct ggcattcgtc attatctggt
    57301 tttatccgct cacggataaa aaattcaaag aaatcgtggt tgaaattgat aatcgtaaaa
    57361 aaatgcagca gcaattaatc agcgatatca ctaat
//
LOCUS       DAZFJL010000013        54983 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFJL010000013 DAZFJL010000000
VERSION     DAZFJL010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.013"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..1981)
                     /locus_tag="ACHJ8C_000787"
     CDS             complement(<1..1981)
                     /locus_tag="ACHJ8C_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFO5550690.1"
                     /translation="MSQLDEAILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWKTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFA
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPMLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAGGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRLVFMDFNRNPEPVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQQMNPLSISLYKMHGYDLITQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLAAPA
                     LASIREIITQAHDNGSGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARMVIDPQGKCLP
                     QTRRGAKEEWRFRSELAEDKNHKLTIQYSQGSFITE"
     gene            complement(1978..3519)
                     /locus_tag="ACHJ8C_000788"
     CDS             complement(1978..3519)
                     /locus_tag="ACHJ8C_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA--acetoacetyl-CoA transferase"
                     /protein_id="HFO5550691.1"
                     /translation="MRKLSTAETLAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDSKGTNRFAHAEMLKRIIAGHFTWSPKMQELVKSNAIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGTIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIDRDILPYMAFRPIIRHPRLMESSLFT
                     PMEDA"
     gene            complement(3529..4305)
                     /locus_tag="ACHJ8C_000789"
     CDS             complement(3529..4305)
                     /locus_tag="ACHJ8C_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HFO5550692.1"
                     /translation="MNDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNEVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQTANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYSHQLLAGLMSATQDCQQATHAFAEKAGVSFHNQ"
     gene            complement(4315..5157)
                     /locus_tag="ACHJ8C_000790"
     CDS             complement(4315..5157)
                     /locus_tag="ACHJ8C_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO5550693.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIARQLAASNINVSGLCRSTYYTAPTLAERKLVIDDNRRALDDAAVLNAA
                     CYMQVVGGLPMGTKDLYEAREQVKQGIRQLLPHSKEVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            complement(5167..5958)
                     /locus_tag="ACHJ8C_000791"
     CDS             complement(5167..5958)
                     /locus_tag="ACHJ8C_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFO5550694.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHATQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            6171..6818
                     /locus_tag="ACHJ8C_000792"
     CDS             6171..6818
                     /locus_tag="ACHJ8C_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO5550695.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYISNP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKQNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTARE"
     gene            complement(6802..7440)
                     /gene="ytfB"
                     /locus_tag="ACHJ8C_000793"
     CDS             complement(6802..7440)
                     /gene="ytfB"
                     /locus_tag="ACHJ8C_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO5550696.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTHTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GADKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            7658..8278
                     /gene="fklB"
                     /locus_tag="ACHJ8C_000794"
     CDS             7658..8278
                     /gene="fklB"
                     /locus_tag="ACHJ8C_000794"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO5550697.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            8587..9999
                     /gene="cycA"
                     /locus_tag="ACHJ8C_000795"
                     /gene_synonym="dagA"
     CDS             8587..9999
                     /gene="cycA"
                     /locus_tag="ACHJ8C_000795"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO5550698.1"
                     /translation="MVDQVKVVADDQSPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPGLSDWVASLSVIILLLVLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFSLIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVLVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(10044..10706)
                     /gene="ytfE"
                     /locus_tag="ACHJ8C_000796"
     CDS             complement(10044..10706)
                     /gene="ytfE"
                     /locus_tag="ACHJ8C_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO5550699.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVDVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMEHISLENNVLFPRALA
                     GE"
     gene            complement(10814..11779)
                     /gene="ytfF"
                     /locus_tag="ACHJ8C_000797"
     CDS             complement(10814..11779)
                     /gene="ytfF"
                     /locus_tag="ACHJ8C_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO5550700.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLSCVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACCWLNMQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(11887..12747)
                     /gene="qorB"
                     /locus_tag="ACHJ8C_000798"
     CDS             complement(11887..12747)
                     /gene="qorB"
                     /locus_tag="ACHJ8C_000798"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO5550701.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQVLTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAADVKFIAYT
                     SLLHADSSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAVAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKPIT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            12836..13216
                     /gene="ytfH"
                     /locus_tag="ACHJ8C_000799"
     CDS             12836..13216
                     /gene="ytfH"
                     /locus_tag="ACHJ8C_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO5550702.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(13345..15288)
                     /gene="cpdB"
                     /locus_tag="ACHJ8C_000800"
     CDS             complement(13345..15288)
                     /gene="cpdB"
                     /locus_tag="ACHJ8C_000800"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO5550703.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLFGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAVFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            15478..16218
                     /gene="cysQ"
                     /locus_tag="ACHJ8C_000801"
     CDS             15478..16218
                     /gene="cysQ"
                     /locus_tag="ACHJ8C_000801"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO5550704.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPIIGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(16208..16765)
                     /gene="ytfJ"
                     /locus_tag="ACHJ8C_000802"
     CDS             complement(16208..16765)
                     /gene="ytfJ"
                     /locus_tag="ACHJ8C_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO5550705.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            17090..17296
                     /gene="ytfK"
                     /locus_tag="ACHJ8C_000803"
     CDS             17090..17296
                     /gene="ytfK"
                     /locus_tag="ACHJ8C_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO5550706.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(17374..18717)
                     /gene="paeA"
                     /locus_tag="ACHJ8C_000804"
     CDS             complement(17374..18717)
                     /gene="paeA"
                     /locus_tag="ACHJ8C_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO5550707.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(19040..19678)
                     /gene="msrA"
                     /locus_tag="ACHJ8C_000805"
     CDS             complement(19040..19678)
                     /gene="msrA"
                     /locus_tag="ACHJ8C_000805"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO5550708.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            19884..21617
                     /gene="tamA"
                     /locus_tag="ACHJ8C_000806"
     CDS             19884..21617
                     /gene="tamA"
                     /locus_tag="ACHJ8C_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO5550709.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSFDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            21614..25393
                     /gene="tamB"
                     /locus_tag="ACHJ8C_000807"
     CDS             21614..25393
                     /gene="tamB"
                     /locus_tag="ACHJ8C_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO5550710.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEAAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVSTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYAISMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNIDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     TVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTEGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            25396..25737
                     /gene="ytfP"
                     /locus_tag="ACHJ8C_000808"
     CDS             25396..25737
                     /gene="ytfP"
                     /locus_tag="ACHJ8C_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO5550711.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26117..26647)
                     /gene="ppa"
                     /locus_tag="ACHJ8C_000809"
     CDS             complement(26117..26647)
                     /gene="ppa"
                     /locus_tag="ACHJ8C_000809"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO5550712.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            26957..27913
                     /gene="ytfQ"
                     /locus_tag="ACHJ8C_000810"
     CDS             26957..27913
                     /gene="ytfQ"
                     /locus_tag="ACHJ8C_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO5550713.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            28053..29555
                     /gene="ytfR"
                     /locus_tag="ACHJ8C_000811"
     CDS             28053..29555
                     /gene="ytfR"
                     /locus_tag="ACHJ8C_000811"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO5550714.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKLQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            29569..30591
                     /gene="ytfT"
                     /locus_tag="ACHJ8C_000812"
     CDS             29569..30591
                     /gene="ytfT"
                     /locus_tag="ACHJ8C_000812"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO5550715.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RNRDKT"
     gene            30578..31573
                     /gene="yjfF"
                     /locus_tag="ACHJ8C_000813"
     CDS             30578..31573
                     /gene="yjfF"
                     /locus_tag="ACHJ8C_000813"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO5550716.1"
                     /translation="MIKRNLPLMITIGVFVLGYIYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGILLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(31606..32604)
                     /gene="fbp"
                     /locus_tag="ACHJ8C_000814"
     CDS             complement(31606..32604)
                     /gene="fbp"
                     /locus_tag="ACHJ8C_000814"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO5550717.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTAVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDQMVEDVERFIREFPDA"
     gene            32780..34153
                     /gene="mpl"
                     /locus_tag="ACHJ8C_000815"
     CDS             32780..34153
                     /gene="mpl"
                     /locus_tag="ACHJ8C_000815"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO5550718.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(34311..34862)
                     /gene="yjgA"
                     /locus_tag="ACHJ8C_000816"
     CDS             complement(34311..34862)
                     /gene="yjgA"
                     /locus_tag="ACHJ8C_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO5550719.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            34956..36308
                     /gene="pmbA"
                     /locus_tag="ACHJ8C_000817"
     CDS             34956..36308
                     /gene="pmbA"
                     /locus_tag="ACHJ8C_000817"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO5550720.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPYAGVADKELLAFDAPDLDLFHPADVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGAVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIVKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            36491..36877
                     /gene="cybC"
                     /locus_tag="ACHJ8C_000818"
     CDS             36491..36877
                     /gene="cybC"
                     /locus_tag="ACHJ8C_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO5550721.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(36922..37386)
                     /gene="nrdG"
                     /locus_tag="ACHJ8C_000819"
     CDS             complement(36922..37386)
                     /gene="nrdG"
                     /locus_tag="ACHJ8C_000819"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO5550722.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(37544..39682)
                     /gene="nrdD"
                     /locus_tag="ACHJ8C_000820"
     CDS             complement(37544..39682)
                     /gene="nrdD"
                     /locus_tag="ACHJ8C_000820"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO5550723.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNSSLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDTSRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(40076..41731)
                     /gene="treC"
                     /locus_tag="ACHJ8C_000821"
     CDS             complement(40076..41731)
                     /gene="treC"
                     /locus_tag="ACHJ8C_000821"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO5550724.1"
                     /translation="MTHLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRYPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLTILASKSRDNSRTPMQWTNGDNAGFTAGEPWIGLGDNYQEINVEAALA
                     DESSVFYTYQKLIALRKQEAVLTWGDYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     TQPWQPGKMLGNWQLVMHNYEEASPQPCTMTLRPFEAVWWLQK"
     gene            complement(41781..43202)
                     /gene="treB"
                     /locus_tag="ACHJ8C_000822"
     CDS             complement(41781..43202)
                     /gene="treB"
                     /locus_tag="ACHJ8C_000822"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO5550725.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIHPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(43321..44268)
                     /gene="treR"
                     /locus_tag="ACHJ8C_000823"
     CDS             complement(43321..44268)
                     /gene="treR"
                     /locus_tag="ACHJ8C_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFO5550726.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKTHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            44453..44506
                     /gene="mgtL"
                     /locus_tag="ACHJ8C_000824"
     CDS             44453..44506
                     /gene="mgtL"
                     /locus_tag="ACHJ8C_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO5550727.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            44647..47343
                     /gene="mgtA"
                     /locus_tag="ACHJ8C_000825"
     CDS             44647..47343
                     /gene="mgtA"
                     /locus_tag="ACHJ8C_000825"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO5550728.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNAAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNLAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(47551..47937)
                     /gene="ridA"
                     /locus_tag="ACHJ8C_000826"
     CDS             complement(47551..47937)
                     /gene="ridA"
                     /locus_tag="ACHJ8C_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO5550729.1"
                     /translation="MSKTITTENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(48010..48471)
                     /gene="pyrI"
                     /locus_tag="ACHJ8C_000827"
     CDS             complement(48010..48471)
                     /gene="pyrI"
                     /locus_tag="ACHJ8C_000827"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO5550730.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(48484..49419)
                     /gene="pyrB"
                     /locus_tag="ACHJ8C_000828"
     CDS             complement(48484..49419)
                     /gene="pyrB"
                     /locus_tag="ACHJ8C_000828"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO5550731.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDSNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(49423..49557)
                     /gene="pyrL"
                     /locus_tag="ACHJ8C_000829"
     CDS             complement(49423..49557)
                     /gene="pyrL"
                     /locus_tag="ACHJ8C_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO5550732.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(49647..50135)
                     /locus_tag="ACHJ8C_000830"
     CDS             complement(49647..50135)
                     /locus_tag="ACHJ8C_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFO5550733.1"
                     /translation="MKEYDDYSAKEKKQLAVCQRLITEKSYLSQEEIRRDLQNHGFDS
                     ISQSTVSRLLKLLGVIKIRNTKGQKIYSVNPQLLPTPDAGRSVAEMVLSVEHNGEFIL
                     IHTVAGYGRAVARILDFHALPEILGVIAGSNIVWVAPRVVKRTALVHKQINYLLKLNI
                     YS"
     gene            complement(50251..51654)
                     /locus_tag="ACHJ8C_000831"
     CDS             complement(50251..51654)
                     /locus_tag="ACHJ8C_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFO5550734.1"
                     /translation="MGKFKFPTAYTILFVLIALVAAMTWIVPAGKYQMVTNAALGKEV
                     PVAGTYAPVEAQPQGITAVLLAPIDGLYNHQTYTAGAIDVALFVLIIGGFLGVVNKTG
                     AIDAGIERVTTRLNGREEWMIPILMALFAAGGTIYGMAEESLPFYTLLVPVMMAARFD
                     PLVAAATVLLGSGIGTLGSTINPFATVIAANAAGIPFTQGILLRVILLLVGYVICVYW
                     VMRYARKVRNSPESSIVADKMAENQAHFLGNRSETMLEFTPTRKAILILFAASFAFMI
                     YGVAVLGWWMAEISAVFLAAAVIVGVIARMGEETFTSTFIDGARDLLGVALIIGIARG
                     IVVVMDNGMITHTILHSAENLVSGLSTTVFINVTYWLEVLLSFLVPSSSGLAVLTMPI
                     LAPLADFAHVQRDLVVTAYQSASGVVNLITPTSAVVMGGLAIARVPWVRYLRWVAPLL
                     LILTMLNMIVLSIAAMI"
     gene            complement(51711..52715)
                     /gene="argF"
                     /locus_tag="ACHJ8C_000832"
     CDS             complement(51711..52715)
                     /gene="argF"
                     /locus_tag="ACHJ8C_000832"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO5550735.1"
                     /translation="MATSLKNRNFLKLLDYTPAEIQYLIDLAINLKAAKKSGNEKQTL
                     VGKNIALIFEKSSTRTRCAFEVAAFDQGAQVTYIGPSGSQIGHKESMKDTARVLGRMY
                     DGIEYRGYGQNIVEELGEFAGVPVWNGLTNEFHPTQILADLMTMLEHAPGKTLPELSF
                     AYLGDARNNMGNSLMVGAAKMGMDIRLVAPKSFWPDEALVTQCREIASVTGARITLTE
                     DVEEGVYDVDFLYTDVWVSMGEPKEAWAERVSLMAPYQINQQVITATRNPEVKFMHCL
                     PAFHNEHTTVGREIEMAYGLKGLEVTEEVFESAHSIVFDEAENRMHTIKAVMVATLGD
                     "
     gene            complement(52739..53671)
                     /gene="arcC"
                     /locus_tag="ACHJ8C_000833"
     CDS             complement(52739..53671)
                     /gene="arcC"
                     /locus_tag="ACHJ8C_000833"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO5550736.1"
                     /translation="MENKPTLVIALGGNALLKRGEPLEAEIQRKNIDLAAKTIAQLTQ
                     HWRVVLVHGNGPQVGLLALQNSAYAHVAPYPLDILGAESQGMIGYMLQQALKNQLPQR
                     EISVLLTQVEVDANDPAFSNPTKYIGPIYDHAQTQVLQAEKGWVFKADGHSFRRVVPS
                     PQPKRIVERDAIQTLIAHDHLVICNGGGGVPVVEKADGYHGIEAVIDKDLSAALLASQ
                     IHADALLILTDADAVYLDWGKPTQRPLAQVTPELLNEMQFDAGSMGPKVTACAKFVSQ
                     CRGIAGIGSLADGPEILAGDKGTLIRLDTPHNHA"
     gene            complement(53682..54902)
                     /gene="arcA"
                     /locus_tag="ACHJ8C_000834"
     CDS             complement(53682..54902)
                     /gene="arcA"
                     /locus_tag="ACHJ8C_000834"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFO5550737.1"
                     /translation="MEKHYVGSEIGQLRSVMLHRPNLSLKRLTPSNCQELLFDDVLSV
                     ERAGEEHDIFANTLRQQGIEVLLLTDLLTQTLDIPEAKSWLLETQISDYRLGPTFATD
                     VRTWLAEMSHRDLARHLSGGLTYSEIPASIKNMVVDTHDINDFIMKPLPNHLFTRDTS
                     CWIYNGVSINPMAKPARQRETNNLRAIYRWHPQFAGGEFIKYFGDENINYDHATLEGG
                     DVLVIGRGAVLIGMSERTTPQGVEFLAQALFKHRQAERVIAVELPKHRSCMHLDTVMT
                     HIDIDTFSVYPEVVRPDVNCWTLTPDGHGGLKRTQESTLLHAIEKALGIDQVRLITTG
                     GDAFEAEREQWNDANNVLTLRPGVVVGYERNIWTNEKYDKAGITVLPIPGDELGRGRG
                     GARCMSCPLHRDGI"
BASE COUNT        13101 a        13939 c        14516 g        13427 t
ORIGIN      
        1 cttcggtaat aaaagaacct tgcgaatatt gaattgttaa tttgtgattt ttgtcttcag
       61 cgagttcaga gcgaaaacgc cattcttctt ttgcgccgcg acgagtttgt ggtaggcatt
      121 tgccttgtgg atcaataacc atacgcgccc cacggctacc accaccagca tcaatataat
      181 gcgttaattg tgttaatacc gcggcagaag tcagcgccat atgccgccac ataaacagct
      241 cggcaacttc ccccacatgt ttgattgcca atccatgccg ttgcacaaat tcactcaata
      301 gcagggcatc acgagtggcg cgtcggactt tatcggcatg acaaataaat cccgcatggt
      361 cagacattcg tgcctgaatt ttttctctca caaccgacaa cggcatcccg gacccgttat
      421 cgtgcgcctg agtaatgatc tctcttatag aagccagtgc cggagctgcc agctgtgcaa
      481 tatctccatc aatattccgc ttttgcgtgc aaccaataaa gcgcgccaga cgaacagcaa
      541 aaacctgccc ggcattcaac gccgcgccgc caggtcgggt gacgccgtgt gtgccagcaa
      601 cttcccccac cgcaaaacaa ccgggcagag atgtttgtcc ccagatatcg acttcaatgc
      661 cgccattcat gtgctgatta ttcatggcaa actgcaatgg ctgcgtgatg agatcgtaac
      721 cgtgcatctt atacagcgag atagacagag gattcatttg ttgcaggcgt tcgatgggcg
      781 atggtgccag agcgtcgtta ttttccagat acgcgcgaac gtcgtcgtcc agtcgatcta
      841 atgagaacgg caggtcaccc ggcacaggtt ctggattacg attgaaatcc ataaatacca
      901 gacgccctga ttgctgctct tgcgctactg ccatatctaa caagctggag ccaaaatcca
      961 ttacccgcgt ggcgtggaac ggccactgat agcctttacg gaaaatgttt gaagccagct
     1021 cctgggtggt gcgatagtaa tccgcgagga agttatactc gttgcccccg gcatccacgg
     1081 aatagatata tgggatcacc tgtacatagg tgccggacaa attccacgga aacgtgctgc
     1141 gcggtgtgcc gataccaaac tggctttcag tcagattggt tagcgtcaga ccttcctcca
     1201 gcgccagccc cagcgagccg aaacatttgt gtggatacac actgtcgcga taaagctcgc
     1261 caggccctcc tgttgccagt accacattgg gcgcagtcac aattgccagc ccccatgggt
     1321 tatgggcgcg atgaccggtc gcgaggattg cccccgccac gcggtcttcg ccgttttcgt
     1381 cacgctgatg tagcagttta atcactgttg cactggtcaa cattgggata gcaagacgct
     1441 gtacttcttc cagcagcact ttcaccatta accttgaggt acgcggccca cacgaggttg
     1501 cacgcccggc ttcgtcgtgg tcggtttgat aacgaagaat cgcgccatag cgatcttccg
     1561 gtaattccag accaagatat tgcaacccgc caagagtgtg cagagaacct accgcttcaa
     1621 cataagcggt gtcgtgatcc atcgccccac cgctcgccag tgcctctgcc agtttggcga
     1681 agttgtcgcc gttgcccgcg gtagcggcgg taaacagtgt ttgtttatca gagccagaac
     1741 atgccgacgt ccccatatat aacccggcgg tggcgattaa cacattttgc tggcggcgtt
     1801 taagttccac agctgcacgt agcccggctg caccactgcc gactaccagg gcatctgttt
     1861 tccagagtgg gtaatcaaca ccgtctaccg tgacaaccca tgcgggttct gcctgtgcaa
     1921 aaccctttgg gaaagtaacg tgtgtcagtg catcaaggat ggcctcatca agctgactca
     1981 tgcatcctcc attggcgtaa acaggctact ttccatcagg cgtgggtgcc tgataatcgg
     2041 acggaaggcc atatacggca gaatgtcgcg gtcaatctct acgccagggg cgatctcaat
     2101 taattcaacg ccttctgccg ttaactggaa taccgcacgt tcggtgatat acagcacttc
     2161 ctgaccgctt tgctgtgcgt aggcggcgct aaaagtaatt ttttccacct gggtaaccag
     2221 tttggggatt tgacctgact gggcgatatg caagccatct ggcgttacat caatcttgct
     2281 gcctttagcg tcgaacgtac cgcaaaacac cactttacgg gcgttttgtg caatttcgag
     2341 aaatccgccg gggccaatga gattgccatt aagatgggag acgttgacgt taccgtattg
     2401 atccatctct cccatgccga ggaaggtgat atcgatgccg ccgccactgt agaattcaaa
     2461 ttgatcgagt gatggaataa tggcatcggc gttacgggcg cagccgaaca gagcatcatc
     2521 cagcatcatg ccgttatgca caccctgttc tacactcagc cacaaacttt gataaggcac
     2581 gccttcgcgc agcgcaaccc caggaatacc gcccggaata ccaaagccaa agttggttga
     2641 agcgcctgcc accagttccc gcgctgcccg acgggcaatc aggcgacgca ccggatgact
     2701 aaccagttca atagcgtcgt tagaacttag acggcgatgt tgaccgctga tggtcaggtc
     2761 gtaaccgcca agataggttt gcggttgttc gcggtgctca acgatgccat cgaccagaat
     2821 tcctggtagt ttgacccgac gtggttcaat ggtacctgct tccagcacat cgcggacctg
     2881 tacgaacact ttgccgccgc tgttgtgtgc tgccagcgcc atcgaatagc tatccatatc
     2941 aatcgcctct tcttcgaggc tgacgttgcc acgcggatcg gcataggtgc cgcgcaaaat
     3001 cgcgtagtcc actttgaaag ggcgataacg aagtttggtc tcaccgtcga tttcaatcag
     3061 ttctaccagg tcgtcggtag tgcattcatt cgacttaccg ccaccattgc gtggatcaac
     3121 aaacgatccc agcccaacgt gggtaaaaag ccccggacgt ccggcaccga tctcccgcag
     3181 cagtgcctga attacgccac cagggaaaca gtaggcttca atagcattgc ttttcaccag
     3241 ttcctgcatc ttgggcgacc aggtaaaatg tccggcaata atgcgtttga gcatttcggc
     3301 gtgagcaaaa cggttagtgc ctttgctgtc gcgatcgcca atacccagcg agtggattaa
     3361 tgtcagatcg cgcgggtgtc cggtttgcag aaagcgcgct tcaatggcgg ccagaatatg
     3421 gtcagcttcc accatgccgc cgccgttacc gctaatggca atggttgcgc cgtcctgaat
     3481 ttgcgccgcc agtgtctcgg cggttgataa cttacgcata caatttcctt attgattgtg
     3541 gaagctcacg ccggcttttt cggcaaatgc gtgcgtcgcc tgttggcaat cctgagtggc
     3601 ggacattaat ccagccagta actggtggga gtaatcggcg tgtttatttt ccagcgccag
     3661 aatcagttgc ttaatatgtg ccagtgccac cggaccacat ttttccagct gcgctaactg
     3721 gttcgccatc cagctttcca cctgttcttt ttctaccact tcatcaatta aattcgctgt
     3781 ctgagcttcc tgcgccgtca ggcgtttacc cagcatcagc atctggcgac cgaccaccgg
     3841 gccaacctga ttaagcacgc gttcaatgcc catccagcca ggcaccatgc cgagcatcac
     3901 ttccgggtta gagaatttcg cgccggggcg agcgatacga atatcacagc acaaggcgag
     3961 ctcaatccca ccgccaatgg tatgaccgtt gagatttgca accgttaatt ggggtaaatt
     4021 gcgcaggcga ttaaaaacct cgttaccgcg tttaatccat tttctgccca tatctaatgg
     4081 cgaataggcc gaccacgatt taatatcgcc gcccgcacaa aagaatttat cgccggttgc
     4141 cgttaattcg actaatcgta atgtggtgtc gttttcaatt tcatttaaat aatggtcgag
     4201 agattcgatc atttcttcat taatagcatg acatttatct tcacggttca atgtcagacg
     4261 gcaagaagcc gccgctctgc tgaacagaac gggttggtca ttcatgatta ttccttagca
     4321 ataatgcgcg atgcgatcga cgctaatatc cagtgtgctg ttaatatctt tttgccacca
     4381 gtacggtgag aatatttcca gttcaatagc gccattaaat ccggcgtttt caaccagccg
     4441 acgaattgac ggaatattga tgacaccatc tcccggcatt ccacggtcat tgaccagatc
     4501 ggtggtcggc actaaccagt cggaaacatg aaacgcgagg atgcgttttc cggcacgcag
     4561 gatctgactg gcgagatccg ggtcccacca gacatggtag acatctaccg ccacgccgag
     4621 gccaaattcg ccatcgggat cgagttcatc acaccagtcc agcgcctgac gtaacgtgca
     4681 cagacaagaa cgatcggcag cagtcatcgg gtgcagtggc tccagtgcaa ttggcacgcc
     4741 gacctctttc gaatgtggca gcaactggcg aatcccttgt ttcacctgtt cccgtgcttc
     4801 gtacagatct tttgttccca ttggcagacc gccaaccacc tgcatataac aggcggcgtt
     4861 cagtacagcg gcgtcgtcca gcgcccggcg gttatcgtca atcaccagtt tgcgctccgc
     4921 cagcgtcggt gcggtgtagt aggtgctacg gcacaaaccg gaaacgttta tgttactggc
     4981 agcaagctgg cgggcgattt gctggagatc ttcaccctgc aactccctgc gccagggggc
     5041 aatggcgcca ataccgcgtg cggcgcaggc atcaataatg gcgggcagcg gcgttttata
     5101 gcccagtgtc gcggtattga tactgaataa atccgggcgt tgtaacagat ctctcatgat
     5161 tcagcctcag aagcgcggca tacttaagcc accgtcaatg gcgaccgcct gaccacaggt
     5221 gtaaataagt ttaccttccg ccatcgcgcg gacagtggaa gcaatatccg ccggataacc
     5281 ccaacgtccc cacggcacca gacctttggc aatcagttcg tcgtaatagg ccgttgccgg
     5341 aatagtcata tcggttttga tgagaccagg acgcacttca tatacgccaa tttgttcatt
     5401 acaaaggcga gcagcaaaca gacgagcagc ggcagagacg gcagttttgg caatggtata
     5461 ttcgccgcga ttcatcgcca gcatgatggc gttgatggaa ctgacaaaaa taatcgagcg
     5521 gtgcggcagt tctgcttccg gtttcggctg tgccagtaag cgcttactga atgcctgagc
     5581 aaggaagaac ggtgcgcggg tgttaatggc aatattttgg tcgaagctgt ctggttcgag
     5641 gtcgagaagg tcaccgcgtt ttttcactga aatgcctgcg ttgttgagca ggcagtccag
     5701 acgtccccac tggttttgcg ctgcgtcgag catctcttca tgcagggaga gatcgccgac
     5761 atcggcaggg aagcaaataa cttccacgcc ttccgccaga cattcttgtt gtgtggcatg
     5821 taatttttct acgctatccg catccggacg atcattaatc agcagattaa atccgccacg
     5881 cgcaagctcc agcgcacagc cctttccaat accacgagcg gctccggtaa tcacggcaac
     5941 aggacgagtg gatttcatac aatagctctc tttttttgaa ttttgtttcc ttagtgatga
     6001 ttagcttgcg actggaggat aaaaagatca accatctagt tggttgatat gcgatggcga
     6061 tcacaggaca tttgataacc tgatgaaagt acaagttttt gccctttttt ctgaagctga
     6121 aatcaaatca ggttatgata gtcaccacga agaggtcgtg gggaagcgtt atgtatcttg
     6181 ataccagcaa cgtacagagc ctgaaagaaa agctgctgtt atgcgcggtt aatgaatttg
     6241 ccgaatatgg ttacgaaggg gccagagtcg ataatatcgt caaggccgcg gggtgcagta
     6301 aacagacggt ttatcaccac tttggtaata aagaaaattt atttatcgaa gtactggaat
     6361 atacctggaa cgatattcga caaaaagaaa aggcccttga tttctcagat cttccgccac
     6421 agaaagccat tgagaaaata atcgatttta catgggatta ttatatttct aatccctggt
     6481 ttttaaaaat tgtgcatagt gaaaatcaaa gtaagggtgt gcattatgca aaatcacagc
     6541 gtcttttaga aattaaccat gcgcacttgc agttaatgga atccttattg gatgaaggga
     6601 agaaacaaaa tatttttaag ccagatatcg acccattgca ggtgaatatt aatattgctg
     6661 cgcttggggg atattatttg atcaaccagc atacgcttgg cctggtttat cacatcagta
     6721 tggtttcacc gcaggcactg gaagccagac gtaaggtcat caaagagaca atccttagct
     6781 ggcttttggt tgacccttca tctaccgcgc gcgaataaaa ctgccatccg gctggcgggt
     6841 aaacaggacc tgttgattat tccccgtatc aatggttaag cccgtcacca cgccgctggc
     6901 gttctggcgg attttcacca tctgcccgtt ttgcaaatta ctcagtggct tatccgcgcc
     6961 ttctacctgc gccatggcat agacatcggt agcgggcagt ccgtggtcgc ggaacagctg
     7021 cgccatcgtt ttaccgggtt cgacacgata ggagcgccat tgattatcaa ttccgctatc
     7081 tggctggaat ggttgcgtat gggtggtttg cggctgctct tctggttggc cttcctgtat
     7141 tggttctgga gcgaccggag ccacctgatc aggatcattt tgtggcgtga ctaattgcgc
     7201 ccgcagctgt tcttctgtcg gtggttgtga ttgcgactga atatctaact gtgcttcacg
     7261 cgtcaccacc ggcgcgttgg gcgtatcatc agatggaagc agaaatccga ccaccaatac
     7321 aatggcggca ataatgatcc cccgacgatg catcggcggc agcgggtcca tgaagcgaaa
     7381 attgtccggc gcgtgccaga ctttctccag ggttggtttt agttcaaagc gcccgggcat
     7441 ggtttcctcc tgctccgcgt cttgttcctc aatcatagcc tatgaataag ctaacgctat
     7501 gatgtccgtg gtaaacccgc ctttattata ttcatacggg atattgctat tgtttctttt
     7561 tccccgggat ttgtcatcat tcccgcgaca aagttttacc caaagaagtg tggctgatat
     7621 gctgcccgct actttatacc ctaagaaagg aaatatgatg accaccccaa cttttgacac
     7681 catcgaagcg caagcaagct acggcattgg tttgcaggta gggcaacaac tgagtgaatc
     7741 tggcctggaa gggctgctgc cagaagcact ggttgcaggc attgccgatg cgctggaagg
     7801 caaacatccg gctgttccgg ttgatgtggt gcaccgcgcg ctgcgtgaaa tccacgaacg
     7861 cgccgatgcc gttcgtcgtc agcgtttcca ggcgatggct gctgaaggtg tgaaatacct
     7921 ggaagaaaac gccaaaaaag aaggtgtaaa tagcaccgaa tctggcctgc aattccgcgt
     7981 gatcaaccag ggtgaaggcg caattccggc acgtaccgac cgcgttcgtg ttcattacac
     8041 cggaaaactg atcgacggca ccgtgtttga cagctccgtt gctcgcggcg agccagctga
     8101 attcccggtt aacggtgtga tccctggctg gattgaagca ctgactctga tgccggtagg
     8161 ttctaaatgg gaactgacta tcccgcagga actggcatat ggcgagcgcg gcgctggcgc
     8221 gtccatccct ccgttcagca ccctggtgtt tgaagtcgaa ctgctggaaa tcctctaagc
     8281 agcgcattct gttcccctcg aaccagaggg gagcaggcat tcagcaataa acccttcagt
     8341 ttgccaaacg gcgctatttt atgttgcaaa gaccctgtaa gcgtgtattt ttgtgagctg
     8401 tttcgcgtta tcaccgtgat atgacactca ctttaaacat aaaattaaca ttagatctaa
     8461 atcttagtat tcatcccgcg tattgttacc taatatcgat gagtcccgat acagattcgt
     8521 cgtatcatag actgactaaa ggccgtagag cctgaacaac acagacaggt acaggaagaa
     8581 aaaaacatgg tagatcaggt aaaagtcgtt gccgatgatc agtctccggc tgaacagtcg
     8641 ctacggcgca atctcacaaa ccgacatatt cagcttattg ccattggcgg tgccattggt
     8701 acagggttgt ttatggggtc cggcaaaacg attagccttg ccgggccgtc gatcattttc
     8761 gtttatatga tcatcggttt tatgctcttt ttcgtgatgc gggcaatggg ggaattgctg
     8821 ctttcgaatc tggaatacaa atcttttagt gacttcgctt ccgatttact cgggccgtgg
     8881 gcaggatatt tcaccggctg gacttactgg ttctgctggg ttgtaaccgg tatggcagat
     8941 gtggttgcca ttaccgccta tgcgcaattc tggttccctg ggctttctga ctgggttgct
     9001 tcgttatccg tgatcattct gttactggtt ctaaacctcg ccacggtaaa aatgttcggt
     9061 gagatggagt tctggtttgc gatgatcaaa atcgtcgcca tcgtgtcgct gattgttgtc
     9121 ggcctggtca tggtggcgat gcactttcag tcaccgaccg gtgtggaagc atcatttgca
     9181 catttgtgga atgacggcgg ctggttcccg aaaggcttaa gcggcttctt tgccggattc
     9241 cagatagcgg ttttcgcttt cgtggggatt gagctggtag gtacaacagc tgcggaaacc
     9301 aaagatccgg agaaatcact gccacgcgcg attaactcta ttccgatccg tatcattatg
     9361 ttctacgtct tctcgctgat tgtgattatg tccgtgacgc cgtggagttc ggtagtcccg
     9421 gagaaaagcc cgttcgttga actgtttgtg ttggtaggtt tgcctgcggc tgccagcgtg
     9481 atcaactttg tggtgctgac ctctgcggcg tcttccgcta acagcggtgt cttctctacc
     9541 agccgtatgc tgtttggcct ggcccaggaa ggtgtggcac cgaaagcgtt cgctaaactc
     9601 tctaagcgcg cagtacccgc gaaagggctg accttctctt gtatctgtct gctcggcggc
     9661 gtggtgatgt tgtatgtgaa tcccagcgtg attggcgcgt tcacgatgat tacaaccgtt
     9721 tccgcgattc tgtttatgtt tgtctggacg attatccttt gctcgtacct ggtgtatcgc
     9781 aaacagcgtc ctcatctgca tgagaagtcg atctacaaga tgccactcgg caagctgatg
     9841 tgctgggtat gtatggcgtt ctttgtgttt gttctggtgt tgttgacact ggaagatgac
     9901 acccgccagg cgctgctggt taccccgctg tggtttatcg cgctggggct gggctggctg
     9961 tttattggta agaaacgggc tgctgaactg cggaaataat cgcattatca tgctggatgg
    10021 cgcaatgcca tccagctttt agatcactca cccgccagcg cgcgcgggaa tagtacattg
    10081 ttttccagac tgatgtgctc catcaggtca tcaatcagtt cattaatgcc gttatacatc
    10141 gctttccagg tggtgcaggc ttccggcggc ggtgtgacgt tattggtggt gtgtttgatc
    10201 acttccagca gttcgcccgc ttcatcgtgc tcgctttcca ttacgctgat tggccccatt
    10261 gcctggctgc ccatgccttg tttaatcatc gggaagagaa tttgctcttc tttcatcata
    10321 tggctggaaa gctcttcatg cagcatagtc aggtattttg tcagcccttt tggcacactt
    10381 ggtttgtcgg cgtgaacgcg ctcgacttta gtcgcttgca ggatcagctc cggcagctgc
    10441 tcgcgatggc gatcgtgata gcgcacaata atgtggtcga tgatttccgc cagcggcgcg
    10501 ctacgccagt ctttttcaat cggttgttca gcaagctttg ccagttcggc ttcaatgacg
    10561 tcaacatcca gttctttacg tgccgccgcg cgcgccagcg tctgcttacc gccacagcag
    10621 taatccatat catatttacg aaacagagcc gaagcgcgag gaatagagag cgccagttca
    10681 cctaaaggtt ggtcgcgata agccatagct gatacctcat tcttaataag ataagatgca
    10741 ttttaaatgc atctttaagg caaaagctat aacccttacg tagcagaggc ttatttaact
    10801 cactgccagc gtgtcaactt tctgagacag attcagttaa aggtttttct ggttttgctc
    10861 tgaccgcgat gaccacgcca atcactaaca gcgcgatacc gctcagcgtc attagcggcg
    10921 gcatttgctg gcggagtaaa aaggtgtaca gcaaacctgc cagcgtttcg aaaacaatca
    10981 gcggcccgag aatcactgtc ggtaatcgct ggctggcgac gttccagcag agtgcgccaa
    11041 cccatgagca aagcacggct atcgcaacca tcagactaat aaacaccagc ggacgggggc
    11101 caaagggtaa ggagaagtcc ggcgtttgca tattcagcca gcaacaggcg acgagatagc
    11161 cgatgagaga aaccggcagc gtgaccagcg cctgcgccgt cgcccacatc atcggatgtt
    11221 tgtcgggatt ttcccgcagc cagcgggcgt tgcgcagggc ataccatgcc cagcagacca
    11281 cggaaactaa cgctagcacg atgcctgagg tataacgtgc ccagtcaaaa tcggggagtc
    11341 cgtggtttaa ctcagcaata ttcacacacg acaggccgat gccaatacaa accagtgccg
    11401 gggcgagttt tccccatgcg agtttgccgt cgcgctggct ataaagcaga ttggcaaaaa
    11461 cgggaatcac caccggcagg gtgccgataa tcatcgtgga aacaggcgcg ccagtacgtt
    11521 gaatggcact tgcaaggcag aagtaataga taaggttgcc catcatggtg agcatcaagg
    11581 cggtaagcca gtcgcgacgc gccaactgac gcagacgcac gcgtcccagc caggcaatgg
    11641 gcagcgcaat taaccctagc gccaggtaac gccccatcga ctgcaacatc gccgggtatt
    11701 ccggtacgat caacggcccg acaaaaataa gcccccacat caaccctgct aacagggcgt
    11761 acagcacgcc gctaatcatt actggcatcc attggtctgt cagaagaaag ttcaggaagc
    11821 agacatgatt gctcccggca tcccgtcatt atcagagagg atgcgggagg atgccacttt
    11881 aattaactag ttattaacat taaaaagatg gcttacgctt tcggctaacg ttgtcgttgg
    11941 gcggccaatc aatttgctaa gcgttttgct gtcatcaaac agaccgcctt tcgatgcgcc
    12001 aacgtcagaa tccgccagca tatccgccag tccatcaggc agtccgacgc ttttcagtgc
    12061 cgcagcgaaa tcggcttcgc tcaggttttg ataggtaatc ggtttgccgc tctgtttggt
    12121 gagttctgcc gctaactgtg tcagtgtcca ggcactatcg cccgccagtt cgtaaacctt
    12181 gccttcatga ccggcttcgc taattacgcg cgccgcagct accgcataat cagcccgcgt
    12241 tgctgaggca atcttgccat cgcccgcggc accgataaat acgccgtgtt ccagtgctgc
    12301 cggggcgctg gcgaggtagt tttcggtgta ccagccgttg cgcagcagag tgtaaacgat
    12361 gccagaatca gccagcattt tctccgtctc gatgtgctca tcggcgaggc cgagcgggga
    12421 ggaatcggca tgtagcaggc tggtataagc gataaatttc acgtcggccg cctttgcggc
    12481 attaataaca ttacgatgct gcggggcacg ttgacccact tcgctggaag agatcagcag
    12541 tagtttttcc actccctgaa gcgcagatgt cagtgcggct tcatcgccgt aatcagcctg
    12601 acgcacggta atgccttgtg ctgtcagtac ctgtgctttt gccggattac gaacgatagc
    12661 cactatttgg ctggcaggaa ccgttttcat caaggattca ataacatagt gaccaagttg
    12721 gccagtggca ccagtaatag cgatcatggg aagtctccat cgttttgtct tgtgtttaaa
    12781 tacgctaaca cttaaactta cttttagtaa gtacgcacaa aaaggtaagt atgaaatgag
    12841 tcaggttagc ctgtcgcaac aactgaaaga gggaaatctc tttgcggaac agtgcccgtc
    12901 gcgcgaggtg ttgaaacacg tcaccagccg ttggggggtg ttgattctgg tggcgctacg
    12961 cgaaggtact catcgcttta gcgacctgcg gcgcaaaatg ggcggggtga gtgaaaagat
    13021 gcttgcgcag tcgttacagg cattagaaca ggatggtttt cttaaccgta tcgcgtatcc
    13081 ggtggtgccg ccgcatgtgg aatatagcct cacgccgctg ggcgagcagg tgagcgaaaa
    13141 ggttgctgca ctggcggact ggattgagtt gaatttgccc gaggtgttag cggtgcggga
    13201 tgaacgtgcg gcataactgc cggatgcgct gcgcttatcc ggactacggt tcatggcttg
    13261 tagggcggat aagatgcgtc atcatcgcat ccgccaataa atgccggatg cggttgatga
    13321 tttgtaggcc gaaaaagaag tgttttactt actcagatcc acctgataaa tcgcaaaccc
    13381 gatatcatcg gtcgcgactt tattcatcgg atactgccct ttctctttaa taaacactgc
    13441 ggctttatct gacggagagg tttcgaaacg gatatccagt ttcttatcgc cagctattgg
    13501 tgctaaacgc cagttgttat ctgccgccgg gtgaatttcc cccgcacgtt tcgactcatc
    13561 agcaatccac gctgccagca ccgagcggtt ctcatccggt gaagcaaaag cgatatggct
    13621 gtcgcccgta ccggcaaatt tgccgccgta agcgcgatag ttattggtgg caacgaggaa
    13681 catggcgttc ggatcaatcg gcttgccgtt aaaggtcagg ttcttaatcc tttccgcatt
    13741 ggcattaatc atctggcact cgccgtcata acgggcgggc tgggtaacat caatctgata
    13801 attcacacca tcaataacat caaagttata ggtgcggaaa ccatcccagt tgatgagtga
    13861 ctgtggtttc gtgctgttag gatcaatctg gttaaactgt ccggcggagc actccagcca
    13921 ctctttcacc tctttaccgc tggctttcac cacaatcagc gtattggggt agagataaag
    13981 atcggcggca ttacggaagg tcaactggcc tttttccacc tccacatagc ttgcggggtc
    14041 atttttgcga ccaccaactt taaacggtgc ggcagctgaa agcactggca gttttgccag
    14101 atccggatcg ccctgaatgt aatgctcgac atacgctttt tgcgcgttgt tcaccacctg
    14161 cacggtcgga tcgtcctgca caagcgccag atagctatac atattgtcgg cagatttacc
    14221 gattggcttg ctgacgaact ggcgtgtggc atcgtgatcg gctttgagtg tttctaccag
    14281 cttgctgtct tccgccgcga gggatttttt attcgcgatg tcgtaaatcg gccgtgcttc
    14341 cgctttcgcc tgcgtcacct gccatttacc gctgtcatta ctgagttgta agtcgaccac
    14401 accaagatga tcgccccaca tgcctggcat taccgccgga acaccattca gcgtgccttt
    14461 cgcgatatca gccccttcga tatcagcaaa atctttacct gggaaaacgg cgtgagcatg
    14521 gccaaacata atggcgttaa cgcccggaat ttcactgagg taataaactg agttttccgc
    14581 catcacttta tacggatcgg cagacagccc ggaatgcgcc agaacgacaa cgacatcggc
    14641 acctttctcg cgcatttcag gcacgtattt gcgcacggtt tcggtaatat cattcaccgt
    14701 cactttcccg aataaattag ctttatccca gcccatgatt tgtggcggga cgacgccaat
    14761 atagccaatc ttcagcgtct gtttttttcc gtctttatcg accacttcgg tgtctttaat
    14821 taaatacggt gtaaacattg gctgtttggt tctggcgtca atgacgttgg catttacata
    14881 agggaatttc gctcccgcca gcgcattttt cagataatcc agaccgtagt taaactcgtg
    14941 gttgccaagc gtgccgacgg tatagtccag cgtatttaat gccttataga ccgggtgaat
    15001 atcacctgct tttaatcctt tcgccgacat gtaatctgcc agcggactcc cctgaatcaa
    15061 atcgccgtta tcgaccagta cgctgttttt cacttcattg cgggcatcgt taatcaggct
    15121 tgccgtacgt accagtccga atttttccgt ggcggtgtct ttgtaataat cgaaatccat
    15181 catgttgcta tgcagatcag tggtttccat gatacgcaga tcgaccgtcg ctgcattcac
    15241 actggcggca atcagcgtgg ccaggagcgt tgcgctaaac ttaatcatca gggacatcct
    15301 tttatcatcg ggaatacgaa agaaaaggga gaataaacgt cttacttata gaacagtgaa
    15361 gaatgccaca attttacgct ttgaaaatga tgacactatc acagttggcg cattcattaa
    15421 cgatagggta taagtaaaac aataagttaa caccgctcac agagacgagg tggagaaatg
    15481 ttagatcaag tatgccagct tgcacggaat gcaggcgatg ccattatgca ggtctacgac
    15541 gggacgaaac cgatggacgt cgtcagcaaa gcggacaatt ctccggtaac ggcagcggat
    15601 attgccgcac acaccgttat catggacggt ttacgtacgc tgacaccgga tattccggtc
    15661 ctttctgaag aagatcctcc cggttgggaa gtccgtcagc actggcagcg ttactggctg
    15721 gtagacccgc tggatggtac taaagagttt attaaacgta atggcgaatt caccgttaac
    15781 attgcgctca ttgaccatgg aaaaccgatt ataggcgtgg tgtatgcgcc ggtaatgaac
    15841 gtaatgtaca gcgcggcaga aggcaaagcg tggaaagaag agtgcggcgt gcgcaagcag
    15901 attcaggtcc gtgatgcgcg cccgccactg gtcgttatta gccgatccca tgcagatgcg
    15961 gagctgaaag agtatctgca acagcttggc gaacatcaga ctacgtccat cggctcttcg
    16021 ctgaaattct gcctggtggc ggaaggacag gcgcagctgt acccgcgttt cgggccaacg
    16081 aatatttggg ataccgccgc tggtcatgct gtggctgcag ctgccggagc gcacgttcac
    16141 gactggcagg gtaaaccgct ggattacact ccgcgcgagt cgttcctgaa tccggggttc
    16201 agagtgtcta tttattaatt aatttatgca gcaggtccat cacttgctgc acttcttcct
    16261 gagtaagcgc cccgtctttg gcccattgca cgcgaccgtc tttatccagc accactacag
    16321 cggaactttc ttcatccagc tgccaggcac cgcgtgcgac gccattgcta tcaacaataa
    16381 actgcgacca gggataaagc tttttattac tctccagact gctgcgcaca aacatccctg
    16441 aacccggaat cgcgtcgtcg gtgttaacaa tggtggtggt ctggtaacga tcgtgcggta
    16501 acttcgctga tttaatcgct tcaatcagcg tcgcgttttt ctcttttgca gaggtgcgac
    16561 cagcaatatg ttgcagtact cgcacttttc ccactaactg cgcgctgttc caggttttgt
    16621 agctaaactg atctttatca agcaccaact cgccacgatc ggtgatgcca atcggcggca
    16681 ctcgctgacc ggtttcgaac tgatgtgcgg aagccatcat cggcaacagc aggcaggtga
    16741 gtgccagaat cttgcgtagg gtcatggtgt ttccttctta tgatatgcag gtgatccgac
    16801 cacttgggtc tgagttttaa tcatatgtgc tatttatcga atttcccgca agtgtgatgc
    16861 cagtttgcgg tcaagcgcac aaatcatatg aaaaatgaat gcttatactg aagaccgcgc
    16921 ttcggtaaaa agataattct gaataattgt aacctttagg taaaaaaagt tatacgcggt
    16981 ggaaacattg cccggatagt ctatagtcac taggcattaa aatttgcgcc tcataatagt
    17041 tgggccgatt gtggcaccgc aaaggcgtaa tactcagcag gagataacaa tgaaaatttt
    17101 ccaacggtac aacccacttc aggtggcgaa gtacgtaaag atcctgttcc gtggacggtt
    17161 atacatcaag gacgttggcg cttttgaatt tgataagggt aagattctta tcccaaaagt
    17221 gaaggataag ctgcatttgt cagtgatgtc cgaagttaac cgtcaggtta tgcgtctgca
    17281 aacagagatg gcttaaccaa agtgctatgc agtaaaaagt gctatgcagt aataagacgg
    17341 ctcctgattc aggagccgtt gatgtttctg gggttatgcg acgctttctt ctttatcttt
    17401 agcgtcaggc agttttggcg aaagggcggt ggctttgctg tcgattcggg tcaccagcag
    17461 ctggtcgatg cggtagttat cgatatccac cacttcaaat ttgtagccgg cgaatttcac
    17521 cgaatcggtg cgtttcggga tcttacgcag cataaacatc ataaagccgc cgatggtttc
    17581 gtagttgccc gactgcggga actcgtcaat atccagcacg cgcatgacgt cgtcaatagg
    17641 cgtgccgccg tctatcagcc atgagttctc atcacgggcg acaatttgct cttccagccc
    17701 ctggccgacc agatcgccca tcagcgtggt catcacgtcg ttgagggtga tgatccccac
    17761 taccagcgcg tactcgttca tgatcaccgc gaagtcttca cctgcggttt taaaactttc
    17821 caacgcctct gaaagggtta acgtatccgg cacaatcagc gtgttgcgaa tttgtacgcc
    17881 gctgttcagt gccaggcttt ggttagccag cacgcggttc agcaggtctt tagaatcgac
    17941 atagccgatg atgtggtcaa tatcttcatt acagacgaga aacttagagt gcggatgttc
    18001 cgcaacctta ttcttcagac tttgctcatc ttcgtggaga tcaaaccaaa tcacgttttc
    18061 acgcggtgtc attgaagacg gaacggtacg ggattccagc tcaaagacgt tttcaatcag
    18121 ctcgtgttcc tgtttacgta acacgcccgc cagcgcaccg gcttccacta ccgcgtagat
    18181 atcatcagaa gtgatgtcat ctttacgtac cattggcagt ttgaaaatac ggaagattat
    18241 gttcgccagt ccgttgaaga accacaccag cggggtgcaa acgtacaggc agaagcgcat
    18301 cgggttgatg atacgcaaag ccacagcttc tggcgcaatc ataccgatgc gtttcggggt
    18361 taaatccgca aacaggataa acatgccagt cactaacgag aaagagagaa taaagctcag
    18421 ttgctcagag agttctgccg acatatagcg ggagaacagg ctgtgaaaag ctggagaaaa
    18481 tgccgcatca ccgacgatac cgccgagaat cgccactgcg ttcagaccga tttggaccac
    18541 ggtaaagaac atgccgggat tttcctgcat attcagaacg cgttgggcat ttatattgcc
    18601 ttcatcagcc agcagtttaa gtttgatttt gcgtgaggcg gcaagcgaga tctcggacat
    18661 cgagaagaac gcacttacag cgatcaaaca gagtatgact aaaatactgt ttaacatatc
    18721 ttatccgacc tttcaggcca gatcctcgga aggggaagtt gattaatttg tgtgaaatac
    18781 acattgaaag ccgattcgaa gaaagtgaac cggcattttc agtggctagt atagcgtaag
    18841 gtactgtaaa gccgccagag ggttaaaatt cagacagctg aaaaatgaaa aactgcctga
    18901 tgcgctatgc cgaacaggcc tacgcagtcc ccacaatata ttgaatttgg acgattttgt
    18961 aggcctggca aggcgtttac gccgtatccg gcataaacaa aatgcacttt atcaacaatc
    19021 tacatttgta cccgtaacgc tatgcttccg gcggcagaca gacgccaatt ccgccaattc
    19081 cacagtaacc atacgggttt ttatgcagat attgctggtg gtcatcttcg gcataataaa
    19141 acggtgtggc gttagcgatt tccgtggtga tgcgacgatc gtcatcggcg gcaagcatcg
    19201 ccgcctgaaa acgttccaga ctggcgcgag cggcggcatc ctgttctggg gtcagcggat
    19261 aaatcgctga acgatactgc gtgccgtggt cattgccctg acgcattccc tgggcgggat
    19321 cgtgattctc ccaaaatact tgtagcaact gctcatagct gatgacggat ggatcgtaaa
    19381 caatgcgcac cgcttcggca tggccagtat cgccggagca cacttcccga taagtcggat
    19441 ttggcgtata gccgccggta tagcctgcgg cggtgctgta aacgccgggt aactgccaga
    19501 acagacgctc cacaccccag aaacaaccca tcgcaaaaat ggcaatctcc attccgtcag
    19561 gtacattggt cattgagtga ccgttgaccg catgcagtgt ggctacgggc atcggggtgt
    19621 tacgtccagg cagggcatcg gcgggggaaa ccagatgctt tttatcaaat aaactcatgg
    19681 tgtcgctctc ccgaaaatcg gtcattgggg ttaaggttgt aacaagaggc gtatttgcac
    19741 acaataacca ccgtgaatag gtctaaagta aaacataaga aatattttgg gtttagtctg
    19801 ctttttaatc catattactg gatttttgtt aagccgttta acggcgttcc aggggcagga
    19861 aaaaaggata ttcaggagaa aatgtgcgct atatccgaca gttatgctgt gtaagcttac
    19921 tctgcttaag cggatctgcc gtcgccgcga acgtccgtct acaggtcgag gggttatcgg
    19981 gacagctgga aaagaacgtt cgcgcgcagc tttctacgat tgaaagtgat gaagtgacgc
    20041 cagaccgtcg ctttcgcgca cgcgtcgatg atgccatccg cgaaggtctg aaagcgctgg
    20101 gctattacca gccgaccatt gaatttgatc tccgtccacc gccaaagaaa gggcggcagg
    20161 tattgatcgc caaagtcacg ccaggcgtgc cggtgttaat tggcggcacc gatgtggtat
    20221 tgcgcggcgg cgcgcggacc gataaagact atttgaaatt gctcgatact cgcccggcta
    20281 ttggtacggt actgaaccag ggcgattatg aaaatttcaa aaagtcctta accagcattg
    20341 cgttgcgtaa aggttatttc gatagcgaat ttaccaaagc gcagctgggc attgcgctcg
    20401 gcctgcataa agccttctgg gatattgatt ataacagtgg cgaacgttac cgctttgggc
    20461 atgtgacctt tgaaggatca caaatccgcg atgaatacct gcaaaatctg gtgccgttta
    20521 aagagggcga tgagtacgaa tcgaaagatc tggcagaact gaaccgtcga ctttctgcta
    20581 ccggctggtt taactcggtg gtggtggctc cacaatttga taaagcgcgc gaaacgaaag
    20641 tattaccatt gacgggcgtg gtttcgccgc gaacagaaaa caccatcgaa accggcgtcg
    20701 gttactctac ggacgtggga ccgcgcgtga aagcgacgtg gaaaaagccg tggatgaact
    20761 cttacggtca cagtttgacc accagtacca gtatttccgc gccggaacag atcctcgact
    20821 tcagctataa aatgccgctg ctgaagaatc cgctggaaca atattatttg gtgcagggcg
    20881 gttttaagcg cactgacctg aacgataccg agtctgactc cactacgctg gtggcttctc
    20941 gctactggga tctctccagc ggctggcagc gtgccattaa cctgcgctgg agtttcgacc
    21001 actttaccca gggtgaaatt accaacacca cgatgctgtt ttatcctggg gtgatgatta
    21061 gccgcacgcg ttctcgtggt ggcctgatgc caacctgggg cgactcgcaa cgctactcta
    21121 tcgactactc caacacggcc tggggctcgg atgttgattt ctccgttttc caggcgcaga
    21181 acgtctggat ccgcacactg tacgatcgcc atcgttttgt gacacgcggc acgctgggct
    21241 ggattgaaac gggtgatttc gacaaagtac cgccggatct gcgtttcttc gccgggggcg
    21301 atcgcagtat tcgcggctac aaatacaaat ctatcgcccc gaaatacgcc aacggtgacc
    21361 tgaaaggggc ctcgaagctg ataaccggat cgctggaata ccagtacaac gtgaccggaa
    21421 aatggtgggg cgcggtgttt gtcgatagcg gcgaagcggt aagcgatatt cgccgcagcg
    21481 actttaaaac cggtaccggt gtcggcgtgc gctgggaatc gccggtcggg ccaatcaaac
    21541 tcgattttgc cgtgccggtc gcggataaag acgaacacgg gttacagttt tacatcggtc
    21601 tggggccaga attatgagtt tatggaaaaa aatcagcctc ggcgtggtta tcgttatctt
    21661 actgttgctg ggatcggtgg cgtttctggt gggcaccacc agcggcctgc atctggtatt
    21721 taaagcggcg gatcgctggg tgccaggact ggatattggc aaggtcaccg gcggctggcg
    21781 tgatctcacc ttgtctgacg ttcgttatga gcagccaggc gtggcggtaa aagcgggcaa
    21841 tctgcatctg gcggtcgggc ttgagtgcct gtggaacagt agcgtttgta ttaatgatct
    21901 ggcgctgaaa gacattcagg tcaacatcga cagtaaaaaa atgcctcctt ctgaacaggt
    21961 tgaagaagag gaagatagcg gtccgctgga tctctccacg ccgtatccca ttaccctgac
    22021 acgggtggcg ctggacaacg tcaacatcaa gattgatgac accacggtat cggtgatgga
    22081 cttcacctcc ggcctgaact ggcaggagaa aaccctgacc ctgaaaccga catcgctgaa
    22141 aggcctgttg attgctttgc cgaaagtggc ggaagcggca caggaagaag tggtcgaacc
    22201 gaagattgaa aatccgcagc cggaggaaaa gccgctcggc gaaacgctga aagatctctt
    22261 ttctcgcccg gtattgccgg aaatgaccga tgtgcatttg ccgcttaacc tgaacattga
    22321 agagtttaag ggcgagcagc tgcgcgtgac gggcgacacg gacatcaccg tgagcaccat
    22381 gctgctgaaa gtgagcagca ttgacggcaa tactaaactg gacgccctgg atatcgactc
    22441 tagtcaaggg atcgtcaacg ccagcggcac ggcgcagctg tcagacaact ggccggtgga
    22501 tatcaccctc aacagcacac tgaacgtgga gccgttgaaa ggtgaaaagg tgaagctgaa
    22561 agtgggcggc gcgctgcgcg aacagctgga gataggcgtt aacctttccg gtccggtgga
    22621 tatggattta cgcgcccaga cgcgactggc ggaagccgga ttgccgctca acgtggaagt
    22681 gaacagcaaa cagctttact ggccgttcac tggtgagaag cagtatcagg cggatgatct
    22741 gaaactgaaa cttaccggca aaatgaccga ttacgcgatc tctatgcgca cggctgttaa
    22801 gggccaggag atcccgccag ccaccattac ccttgatgcc aaaggtaatg aacagcaggt
    22861 caatctcgac aaacttaccg tcgcggcgct ggaagggaaa acggaactta aggcgttgct
    22921 cgactggcag caggccatta gttggcgcgg tgagctaacg cttaacggca ttaacaccgc
    22981 caaagagatc ccggagtggc cgtcgaaact caatggcttg attaaaaccc gcggtagcct
    23041 gtacggcggc acctggcaga tggaggtgcc agaactgaag ctgaccggta acgtcaaaca
    23101 gaacaaagtg aacattgacg gcacgctgaa aggcaacagt tatatgcagt ggatgatccc
    23161 agggctgcat ctggaactgg ggccaaacag tgccgaagtg aaaggcgagc tgggggtaaa
    23221 agatctcaat cttgatgcca ccatcaacgc gccggggctg gataacgcgc tgccggggct
    23281 tggcggtaca gcgaaagggc tggtgaaagt acgcggcacg gtggaagcgc cacaactact
    23341 ggcagatatc accgcgcgcg gcctgcgctg gcaggaactt tccgtggcgc aggttcgcgt
    23401 ggaaggcgac attaaatcca ccgatcagat cgccgggaaa ctcgacgtac gcgttgagca
    23461 aatttcgcaa ccggatgtaa atatcaacct cgtcaccctg aatgccaaag gcagtgaaaa
    23521 gcagcacgag ctacagttgc ggattcaggg cgagcctgtc tccgggcagc ttaatctggc
    23581 aggaagtttt gatcgcaaag aagaacgctg gaagggaact cttagcaata cccgcttcca
    23641 gacgccggtc ggcccgtggt cgctgacccg cgatattgcg ctggattacc gcaataagga
    23701 gcaaaaaatc agcatcgggc cacactgctg gcttaacccg aatgcggaac tgtgcgtgcc
    23761 gcaaactatc gatgcgggtg ccgaagggcg tgcggtggtg aatctcaacc gcttcgacct
    23821 ggccatgctg aaaccattta tgccagaaac cactcaggcc agcggtatct tcacgggtaa
    23881 agcggacgtt gcctgggaca ccacgaaaga ggggctgccg cagggcagta tcaccctttc
    23941 ggggcgtaat gtgcaggtga cgcaaaccgt caacgatgcg gcgctgcccg tggcgtttca
    24001 gacgctgaat ctgacggcgg aattgcgtaa caaccgtgcc gaattgggct ggaccatccg
    24061 cctgaccaat aacggccagt ttgatggaca ggtgcaggtg accgatccgc aaggccgccg
    24121 taatcttggt ggcaacgtca atatccgtaa cttcaacctt gcgatgataa accctatctt
    24181 tacccgtggg gaaaaagcag cggggatggt gagtgccaac ttgcgtctgg gtggtgatgt
    24241 gcaaagcccg cagttgtttg gtcagcttca ggttacgggg gtggatatcg acggcaactt
    24301 tatgccgttt gatatgcaac caagccagct tgcggtcaac tttaacggta tgcgctcgac
    24361 gcttgcaggt accgtgcgga cccagcaggg tgaaatctac ctgaacggtg atgccgactg
    24421 gagccagatt gaaaactggc gggcgcgggt aacagcgaag ggcagtaaag tgcgtatcac
    24481 cgtgccgccg atggtacgaa tggatgtatc gccagatgtt gtattcgagg ctacaccaaa
    24541 cctgtttacc ctcgatggtc gcgtggatgt tccgtgggcg cgcatcgtgg tgcacgatct
    24601 gccggaaagc gcagtaggtg tttccagcga tgtggtgatg cttaacgata acctgcaacc
    24661 ggaagagccg aaaacggcgt cgattccgat taacagtaac ctgactgtcc acgttggcaa
    24721 caatgtgcgc attgacgcct ttggcctgaa agcgcggctg acgggcgatc tcaacgtcgt
    24781 tcaggacaaa caagggctgg gcctgaacgg gcagatcaac atccctgaag ggcgcttcca
    24841 tgcctatggt caggatctga ttgtgcgtaa gggtgagcta ctgttctctg gtccgccaga
    24901 tcaaccgtat cttaatatcg aagctattcg taacccggat gctacagaag acgacgtcat
    24961 cgccggagtt cgcgtcactg gtctggcgga cgaaccgaaa gcggagatct tctctgaccc
    25021 ggcgatgtcg caacaagctg cattgtctta tttgctacgt ggacaagggc tggagagtga
    25081 tcagagtgac agcgcggcga tgacctcgat gctgattggc ctgggggttg cgcaaagtgg
    25141 ccagattgtg ggtaaaatcg gcgagacgtt tggcgtaagc aatttagcgc tcgacaccga
    25201 gggagttggc gactcctccc aggtggtggt cagcggctat gtattgccag gcctgcaagt
    25261 gaaatacggc gtgggtatat ttgactctat agcaacactc acgttacgtt atcgcctgat
    25321 gcctaagcta tatctggaag ccgtgtctgg tgtagaccag gcactggatt tgctctatca
    25381 gttcgagttt tagcaatgcg aatatttgtc tacggcagtt tacgccacaa acaaggcaac
    25441 agtcactgga tgaccaatgc ccagttactg ggcgatttca gtatcgataa ctaccagttg
    25501 tatagcctgg gccactatcc aggcgcagtt ccggggaacg gaacagtaca cggtgaagtt
    25561 tatcgtattg acaacgccac gctggccgaa cttgatgcct tgcgcaccag gggcggtgaa
    25621 tacgcgcgcc agttgattca gacgccgtac gggagtgcat ggatgtacgt ttatcaacga
    25681 cccgtcgatg gattaaagct aattgaaagc ggcgactggt tagacaggga taagtaataa
    25741 taaaagcacg ccaccttcgg gtggcgttgt tttatgcgat gtatctcgcg attctttttg
    25801 taactttccc atcttttgct tttctctccg ggccgctttc catatctgca atcgcataaa
    25861 aaactctgct ggcgttaaca aatgtgcagg ggtaaaacgg gggcacgctt tgaacggtga
    25921 gtatgagtga ggccggctaa cttccagcaa gtggctatga acagcaaaat ggtggtcgag
    25981 cttagcttgt tcttaccgtt caagcctttc aactgctgaa aataataata gtggaataga
    26041 gagtttattt atcgcgagca taaaaaaacc cttactaacc gaagcccggt gttcagggtt
    26101 attgcgccag aagaacttat ttattcttgg cgcgctcgaa ggaagcaacg atttcagctt
    26161 tagcggcttc tgcgttttcc caaccttcca ctttaaccca cttgcctttt tcgaggtctt
    26221 tgtagtgctc gaagaagtga gcgatttgcg ctttcagcag ctctggcaga tcgttaacgt
    26281 ctttaatgtg atcgtattct ttgctcagct tggtgtgcgg aacagcaatc agtttcgcat
    26341 cttcaccggc ttcgtcggtc attttcagta cgccaaccgg acggcagcgg atcacagaac
    26401 ccggctgcag cgggtacgga gtcgggacca gtacgtcaac cgggtcaccg tccagagaca
    26461 gggtgtggtt gatgtaaccg tagttgcacg gatagaacat cgcggtggac atgaagcggt
    26521 caacgaacag tgcgccgctc tctttgtcga tttcgtattt gatcggatct gcgttagccg
    26581 ggatctcaat aacaacgtag atgtcttccg gcagatcttt acccgcaggg acgttgagta
    26641 agctcatgtc tgtttccttt aaaaatatgt ggcaaacaag tgccgagtat tatagccaac
    26701 tcgcgccgaa tgtcttcgct tgttttcgtc ttcattttcc tttttcgaca gttttaagac
    26761 ggtatacaga gcaggaaaat ccataaccgt agccgcattt ttcatagtga gatgaaagcg
    26821 attacaaact tgtgattaac gttttattta cttttttgaa gtgtgatgta acgcaatccg
    26881 ttacataacg aattgtctat agttttttcg cgaacatctt ttcaccaata ataattaccc
    26941 cgacgaggac aaccctatgt ggaaacgctt acttgtagtc tctgcagtct cggcagccat
    27001 gtcgtctatg gcgttggccg ctccattaac cgtaggattt tcgcaggtcg gatcggaatc
    27061 cggctggcgc gccgcagaaa ccaatgtggc gaaaagtgaa gccgaaaaac gcggtatcac
    27121 actgaaaatt gccgatggtc agcaaaagca ggaaaaccag attaaagcgg tacgttcctt
    27181 cgtcgcacaa ggggtggatg cgatctttat cgctccggtg gtggcgacgg gttgggaacc
    27241 ggtattaaaa gaggcgaaag atgccgaaat cccggtcttc ttgcttgacc gttccatcga
    27301 tgtgaaagac aaatctctct atatgaccac tgtcaccgcc gacaacatcc tcgaaggcaa
    27361 gttgattggt gactggctgg taaaagaagt gaatggcaaa ccatgcaacg tggtggagct
    27421 gcagggcacc gttggggcca gcgtcgccat tgaccgtaag aaaggctttg ccgaagccat
    27481 taagaatgcg ccaaatatca aaattatccg ctcgcagtca ggtgacttca cccgcagcaa
    27541 aggcaaagaa gtgatggaga gctttatcaa agcggaaaac aacggcaaaa acatctgcat
    27601 ggtttacgcc cataacgatg acatggtgat tggtgcaatt caggcaatta aagaagcggg
    27661 cctgaaaccg ggcaaagata tcctgacagg ctctatcgac ggcgtgccgg acatctacaa
    27721 agcgatgatt gatggcgaag cgaacgccag cgttgaactg acgccgaaca tggcaggccc
    27781 cgcttttgat gcgctggaga aatataaaaa agacggcacc atgcctgaaa agctaaccct
    27841 gaccaaatcc accctttatc tgcctgatac cgcaaaagaa gagttagaga agaagaaaaa
    27901 tatggggtat tgagggttgc tatgcctgat gccgattcgt aggccggata aggcgctcgc
    27961 gccgcatccg gcggtggtgc gctgaagcct gatgcgacgc ttaccgcgtc ttatcatgcc
    28021 tactgggagc acgctttaca ccgggggaaa ccatgacgac cgaccaacac caggaaatcc
    28081 tccgcaccga aggattaagt aaatttttcc ccggtgtcaa agcgttagac aacgttgatt
    28141 tcagcctgcg ccgtggtgaa atcatggcgc tgctcggtga aaacggggcg ggaaaatcga
    28201 cgctcatcaa agcattaacc ggtgtatacc acgccgatcg cggcaccatc tggctggaag
    28261 gccaggctat ctcaccgaaa aacaccgccc acgcacaaca actcggcatc ggcaccgtct
    28321 atcaggaagt caacctgcta cccaatatgt cggtcgctga taatctattc ataggtcgcg
    28381 aacccaaacg tttcggcctt ctacgccgta aagagatgga aaagcgcgcc accgaactga
    28441 tggcatctta cggtttctcc ctcgacgtgc gcgaaccgct caaccgcttt tcagtcgcga
    28501 tgcagcaaat cgtcgccatt tgccgggcta tcgatctctc cgccaaagtg ctgatcctcg
    28561 atgaacccac cgccagtctc gacactcagg aagtagagtt actgtttggc ctgatgcgtc
    28621 aacttcgcga tcgcggcgtc agcctgatct tcgtcactca ttttctcgat caggtttatc
    28681 aggttagcga tcggatcacc gtcttacgca acggcagttt tgtgggctgt cgggaaaccc
    28741 gcgagctacc gcagatcgaa ctggtaaaaa tgatgctggg gcgtgagctg gacacccacg
    28801 cgcttcagcg tgccgggaga acattgctga gcgacaaacc tgttgccgcg ttcaagaatt
    28861 acggcaaaaa aggaacgatc gcgccgtttg atctcgaagt acgccccggc gagatcgtcg
    28921 gtctggcagg cttgctggga tcaggacgta ccgaaaccgc cgaagtgatc ttcggtatca
    28981 aacctgctga tagcggcacg gcgttgatca aaggcaaact gcaaacgcta cgctcgccac
    29041 atcaggcttc ggttcttggc attggcttct gcccggaaga caggaaaacc gatggcatca
    29101 tcgctgccgc ctcggtgcgg gaaaatatca tccttgctct ccaggcccag cgcggctggt
    29161 tacgacctat ttcccgcaaa gagcagcaag agattgccga acgctttatc cgccagcttg
    29221 gcattcgcac accttccacc gaacaaccga ttgaatttct ctccggcggc aatcagcaaa
    29281 aagtgttgct ttcacgttgg ctactgaccc gaccgcaatt tctgattctc gacgagccaa
    29341 cgcgcggaat tgatgttggt gcccacgccg agatcatccg cctgattgaa acgctgtgcg
    29401 ccgatgggct ggcgctgctg gtgatctcct ccgagctgga agagctggtg ggctatgccg
    29461 accgggtgat tatcatgcgc gatcgcaaac aggtagcgga gatcccgctg gcagagcttt
    29521 ccgttccggc gatcatgaac gccattgcgg cgtaaggaga atagtgtgat gcctcaatct
    29581 ctcccggaca ctacgccgcc gaaaaggcgt tttcgctggc caacaggaat gccgcagctg
    29641 gcggcactgt tgctggtgct gctggttgat agcctggtgg ccccgcattt ctggcaggtg
    29701 gtgcttcagg atgggcgttt gttcggtagc cctatcgaca ttcttaaccg tgcggccccc
    29761 gttgcgctac tggcgattgg catgacgctg gttatcgcca ctggtgggat tgatctctcc
    29821 gtaggggcgg tgatggctat cgccggagcc acaacggctg cgatgacggt cgcgggattc
    29881 agcctgccaa ttgttttgtt aagcgccctg ggcactggca tcctggcggg attgtggaac
    29941 ggcatactgg tagcgatcct caaaattcag ccgtttgtcg ccaccctgat cctgatggtc
    30001 gccgggcgcg gcgtggcgca actgatcacc tccggacaga tcgtcacatt taactcaccg
    30061 gatctctcct ggttcggcag tggatcgctg ttgttcctgc caacgccagt cattattgcg
    30121 gtgctgacgc ttctcctgtt ctggctgttg acccgcaaaa cggcactggg gatgtttatc
    30181 gaagccgttg gtatcaacat tcgggcggca aaaaatgccg gggtaaacac gcggatcatc
    30241 gtcatgctca cctacgtgtt gagcgggcta tgtgcggcga ttgcgggcat tatcgtggcg
    30301 gcggatattc gcggtgccga tgccaacaat gccggattat ggctggagct ggacgccatt
    30361 ctcgcagtgg tgattggcgg cggatcgctg atgggcgggc gctttaacct gctgctttcg
    30421 gtggtggggg cgctgattat tcagggaatg aacaccggaa ttttgctttc tggctttccg
    30481 ccggagatga accaggtggt gaaagcggtg gtggtgcttt gcgtgctgat tgttcagtcg
    30541 caacgcttta tcagtctgat taaaggggta cgtaaccgtg ataaaacgta atttgccact
    30601 gatgatcacc atcggcgtct ttgtgttggg ttatatctac tgtctgaccc agtttcccgg
    30661 ttttgcttcc acaagagtga tctgcaatat cctgaccgat aatgcctttc ttgggataat
    30721 cgccgttggc atgacctttg tgatcctctc cggtgggatc gatctctccg tcggttcggt
    30781 gatcgccttt accggcgtgt ttctggcaaa agtgattggc gatttcggcc tctcgccgct
    30841 gctggcgttt ccgctggtgt tggtgatggg ctgtgccttc ggcgcattta tggggcttct
    30901 gattgacgcc ctgaagatcc cggcgtttat cattaccctt gcgggaatgt tctttttgcg
    30961 tggcgtcagc tatctcgttt cggaagagtc gatcccgata aaccatcctg tttatgacac
    31021 gctctcaagc cttgcgtgga aaatccctgg cggtggtcgc ttaagtgcga tgggactact
    31081 gatgctggcg gtggtggtta tcggcatatt actcgcgcat cgtacccgtt ttggtaatca
    31141 ggtatacgcc attggcggca acgcaacgtc ggcaaacctg atggggattt ccactcgcag
    31201 caccactatc cgcatttata tgctttctac cgggctggcc acgttggcgg ggattgtctt
    31261 ctcgatttat acccaggccg gatatgcact ggcgggcgta ggtgtggaac tggatgctat
    31321 cgcctcagtg gtaattggcg gtacgctttt gagtggtggc gttggaacgg tattagggac
    31381 gctttttggc gtggcgattc agggactgat tcagacttac ataaactttg atggcacgct
    31441 gagttcctgg tggacgaaaa tcgccatcgg tattttgttg tttattttta tagcattaca
    31501 gcgtggatta acggtgctgt gggagaatcg tcagagttcg ccagtgacaa gagtcaacat
    31561 tgcgcagcga taaaaacgcc tctccgtgtg gagaggcgca ggagcttacg cgtccgggaa
    31621 ctcacggata aagcgttcga catcttcaac catctggtcg ttgccgacaa agaacgaacg
    31681 gcgctggtgc agggtttccg ggataatatc cagaatacgc tctttgccat cgctcgcttt
    31741 accgcccgct tgttccgcca ggaatgccat cgggttgcac tcatacagta aacgtagttt
    31801 accgtccggg tggctggcgg tgcttgggta gagataaata ccgcctttca gcaggttacg
    31861 gtggaaatcc gcgaccagtg aaccgatata acgtgaggta tacgggcggt tggtggattt
    31921 atcttcttcc tggcagaatt taatgtactt cttcaccccg ttcggaaact taatgtagtt
    31981 tccttcgttg atggagtagg ttttgccttt ctccgggaag cgcatccgtt cctggcacag
    32041 gcagaaaaca cccagcgaag gatcataagt aaaggcgtga acaccgcatc cggtggtgta
    32101 aaccagcata gtagaggagc cgtataccac gtaacctgcc gcaacctgtt tgttaccagg
    32161 ctggaggaaa tcttcttccg ttaccgccgt gccaacaggc gtaacgcggc ggtagataga
    32221 gaaaatggta ccgacagaga cgttaacatc gatgttggac gagccatcca gtgggtccat
    32281 cagcaccacg tattttgcgt gttcacagcc ttcaaagacg acaatctcat cttcttcttc
    32341 agaggcaatg cccgcaacga tatcgcgtgc tttcagtgcg gctttcagtt tctcattagc
    32401 gaacaggtcg agtttctgct gaacctcgcc ctgcacgttc tcagcaccgc tggcacccag
    32461 gatatcaacc agtcctgctt tgttgatatc gcgatggata atcttggcgc ccagttttat
    32521 tgccgacagc aaagcagtga gctcaccggt agcatgagaa aactcgtgct gcttttcgac
    32581 aataaattca cctaacgttt tcataaaact ttccctgcaa tgtttatgga gtaaagcgac
    32641 cgcaacaatc ttaacaaata atcccaatgt tgcgctcagg tgaatcgcgc cagcaaatta
    32701 cggattatcc tgaaatgcgt ttctcacttg cccgacatat gcgtaaaatg agcgccagat
    32761 taaaaaagga tagtgacgta tgcgcattca tattttagga atttgtggca cgtttatggg
    32821 cggtctggcg atgctggcgc gccagttagg ccatgaagta acgggttcgg acgccaatgt
    32881 gtatccgccg atgagcacct tacttgagaa gcaaggcatt gaactgattc agggttacga
    32941 tgccagccag ctcgatccac agccggatct ggtgattatt ggtaacgcca tgacccgtgg
    33001 aaatccgtgt gtggaagcgg tactggaaaa aaacatccct tatatgtcag gtccgcagtg
    33061 gctgcacgat tttgtgctgc gcgaccgctg ggtgctggcc gttgccggta cacacggcaa
    33121 aaccaccacg gcgggaatgg cgacctggat tctggaacaa tgcggttaca aaccgggatt
    33181 tgtgatcggc ggtgtgccgg ggaactttga ggtttcggcg cgtctgggcg aaagcgattt
    33241 ctttgttatc gaagcggatg agtacgactg cgccttcttc gacaaacgct ctaaatttgt
    33301 ccattactgc ccgcgtacgc tgatcctcaa caaccttgag ttcgatcacg ccgatatctt
    33361 tgacgacctg aaagcgatcc agaaacagtt ccaccatctg gtgcgtatcg ttccggggca
    33421 ggggcgtatt atctggccgg aaaacgatat caacctgaaa cagaccatgg cgatgggctg
    33481 ttggagcgag caggagctgg tgggcgagca aggtcactgg caggcgaaaa agctgaccac
    33541 tgacgcttct gaatgggaag tcttgctgga tggcgaaaaa gtgggcgaag tgaaatggtc
    33601 gctggtaggc gaacataata tgcacaatgg cctgatggcg attgcggcgg ctcgccatgt
    33661 tggtgtagcg ccggcagatg ccgctaacgc gctgggttcg tttattaacg ctcgtcgccg
    33721 tctggagttg cgtggtgaag cgaatggcgt cacggtatat gacgattttg cccatcaccc
    33781 gacggcgatt ctggcaacgc ttgcggcgct gcgtggcaaa gttggtggta cggcgcgcat
    33841 tattgccgtg ctggaaccgc gctcgaatac catgaaaatg gggatctgca aagacgatct
    33901 ggcaccttca ttaggtcgtg ccgatgaagt cttcctgctg caaccggcac atattccgtg
    33961 gcaggtggca gaagtggcag aagcctgtgt tcagcctgca cactggagcg gcgatgtgga
    34021 tacgctggca gatatggtgg tgaaaaccgc tcagcctggc gaccatattc tggtgatgag
    34081 caacggcggt tttggtggga tccatcagaa actgctggat gggctggcga agaaagcgga
    34141 agctgcgcag taattcacag cctcagcctg atagagtgtg tgtaggccgg ataaggcgtt
    34201 cacgccgcat ccggcatttg agcatagtgc ctgatgcgac gcttgatgcg tcttatcagg
    34261 cctacaggtg caccgtatcc ggcaaaccat actcacatca acaacgaaaa ttacccttcg
    34321 ttttccgcta actcacgcag atactggaaa atctggcgcg cggatttcgg cggcttattc
    34381 ccttctttct ctttcttcgc gttgcggatc agagttcgca gttgctgacg atcggcatcg
    34441 ggccacagat tcaatacttc agcgatggcg tcatcaccct ggtcgatcag acgatcgcgc
    34501 aggttttcca gtttatggaa cagcaccacc tgctggttgt gacggttttt cagcttatcc
    34561 agcgcctgac ggatcggctc aacatcgcgc tggcgcagca tcttaccgat gagctgcaac
    34621 tggcggcggc gaccttccat cttaatacgc tgggcgagct caatagccgc gcgcagatcc
    34681 gcatctaacg ggatcttatc cagcgcgttt ttccccagat caacaatttc tgcgcccagg
    34741 cgttttagct cctcggcatc gcgtttaatt tcacttttac tgacccagat aatttcatcg
    34801 tcttcgtctt cgatgtcatc accgggaacg tcgtcgagcc agtcttcggg ctgcttagtc
    34861 atctcaggct ccttaaaaaa agaggctaat gttaccagtt aagatgcgca ctgaaaaacg
    34921 gttctctgtt agacttcaga gaaactctct acattatggc acttgcaatg aaagtaatct
    34981 ctcaagttga agcgcagcgc aagattctgg aagaagcagt ttcgactgcg ctggagttgg
    35041 cctcaggcaa atcggacggt gcagaagttg ccgtcagcaa gaccaccggc attagcgtaa
    35101 gcacgcgtta tggtgaagtg gagaatgttg aattcaatag cgatggcgcg ctggggatca
    35161 ccgtttatca ccagaaccgt aaaggtagcg catcgtctac cgatttaagc ccgcaggcca
    35221 ttgcccgcac cgtacaggcg gcgctggata ttgcccgtta cacttcgcca gatccgtatg
    35281 caggcgtggc agacaaagaa ctgctggcct ttgacgcacc ggatctcgac ttgttccacc
    35341 ctgcggacgt ttctccggat gaagccattg aactggcggc ccgtgctgaa caggcggcat
    35401 tacaggcgga taaacgcatt accaacaccg aaggcggtag ctttaacagc cactacggtg
    35461 tcaaagtttt tggcaacagc cacggcatgt tgcagggtta ctgctcaacg cgtcattcgc
    35521 tctccagttg tgtaatcgcc gaagaaaatg gcgatatgga gcgcgattat gcctacacca
    35581 ttgggcgtgc gatgagcgat ctgcaaacgc cagagtgggt cggggccgac tgtgctcgcc
    35641 gtaccttatc gcgcctgtca ccgcgtaaac tttctaccat gaaagcgccg gtgatttttg
    35701 ccaatgaagt ggcaacaggg ttgtttggtc atttggtggg ggcgatagcg ggtggtgcgg
    35761 tttaccgtaa atcgaccttc ttgctggatt cgctgggtaa acaaattctg ccggactggc
    35821 tgactattga agaacatccg catctgctga aagggctggc atctacacca ttcgacagcg
    35881 aaggtgtgcg caccgagcgc cgcgatatcg tcaaagatgg catcctgact cagtggctgc
    35941 tgaccagcta ttcggcgcgc aaattggggc tgaaaagcac cggacatgcg ggcggtattc
    36001 acaactggcg cattgccgga caaggtctaa gcttcgagca gatgctcaaa gagatgggca
    36061 ctgggctggt ggtgacggaa ttgatgggcc agggcgtgag tgccattacc ggtgattatt
    36121 cccgtggtgc agcgggcttc tgggtagaga atggcgagat tcagtatccg gtgagcgaaa
    36181 tcaccatcgc tggtaattta aaagatatgt ggcgcaatat tgtcaccgtc ggtaacgata
    36241 ttgaaacacg cagtaatata cagtgtggtt ctgtgctgtt gccggagatg aaaatcgccg
    36301 ggcagtaaaa aactggcgcg acctgccgcg ctataaacta aaaaattaca caaaatcatt
    36361 cgcactgcat cgagacggaa agtgaatgag agcaggggag cgtactcgta gtacgtgact
    36421 cgggcgaatg agcgtagccg tcgatgaggc agtgtgaagg atgaagtgta aataaaaaag
    36481 gaagtgagca atgcgtaaaa gcctgttagc tattcttgca gtctcctcat tggtattcag
    36541 ttcggcgtcg tttgccgctg atctggaaga caatatggaa accctcaacg acaatttaaa
    36601 agtggttgaa aaagccgata acgcggcgca agtcaaagac gcgttaacga agatgcgcgc
    36661 tgcggcgctg gatgcgcaaa aagcgacgcc gccgaagctt gaaggcaaat caccggacag
    36721 cccggaaatg aaagacttcc gccacggttt cgacattctg gtcggtcaga ttgacgacgc
    36781 gctgaagctg gcaaatgaag gtaaagtaaa agaagcgcag gctgccgcag agcaactgaa
    36841 aacgacccgc aacgcctatc accagaagta tcgttaattc tttatttccc tgttgcctgc
    36901 actcaggtaa cagggaactt atcatcgcaa atgatgcacc acctgattgc tgctgccgcg
    36961 ccagatcagg gaagggtctt ttaaatcctg cacaaatttg ccgtcgacca gcacgttaat
    37021 caaatcaaca acctgcattt gcgcagcgtt gagttcgtcg agtttatagc ctgtccacac
    37081 ccagatgtct ttacccggac actcggcgcg gatgcgttgt accagtttca gaatatccgg
    37141 cacgttttgc ggatgtagcg gatcgccgcc ggagagggaa ataccctggc gtttgatacg
    37201 agtgtcattc agatcgttaa tgatctggtc ttccattgct ttggtaaatg gctgaccgga
    37261 atttacccgc caggtgcttt tgttatagca accggggcat tcatgaacac acccggagac
    37321 aaacagggtg cagcgagtgc cggggccgtt gacgatgtcg acaggatagt actgatgata
    37381 gttcattatt tcacctggct tatcagtccg cgtgtaggct gcatccggta atcaacgcct
    37441 gatgcgacgc tgtcgcgtct tatcaggcct acagccgttg ccaaatgtag gccggataag
    37501 gcgtttacgc cgcatccggc atttgcttaa cacagagtga agattaacct atctgcccat
    37561 ttcccaaatg tttaacgcgg cgcttaactt cttcctgctt accagcgtta aacggacgtg
    37621 catccgggct acctaaatat ccgcacacgc ggcgagtcac cgacacacgg gaggtgtcat
    37681 ggttaccaca tttcggacaa gtgaagcctt tgctggtgca ctcaaactcg ccggtaaagc
    37741 cacactcgta gcattcatcg atcggtgtgt tggtgccgta atacggtaca tgctgatagc
    37801 tgtaatccca gacatcttcc agcgccttca ggttgtgctg aatgtttgga tactcgccat
    37861 agcaaatgaa gccaccgttc gccagtggcg ggtaaggcgc ttcaaagtcg atcttgtcgt
    37921 acgggttcac cttcttctcc acatcgaggt ggaaactgtt ggtgtagtaa cctttatcgg
    37981 tcacgcccgg caccacgcca aactcagcgg tatcgagacg gcagaagcgg tcacacaggt
    38041 tctcactcgg cgtactgtag aggctgaaac cgtagccagt ttcttctttc cactgatcca
    38101 ccgcctgacg cagacgctca acaatcgcaa tacctttcgc gcgcagctgc tcgttgtcgt
    38161 aaacatgctc gccgccgaac agcgcgttaa tggtttcgtg gatgccgatg taacccagcg
    38221 aaatagacgc acgaccgttt ttgaagattt cagaaacgtc gtcgtcagca ttcagacgca
    38281 cgccacaagc gccttccata tagaggatcg gggctacgcg cgctttcacg ccttcgagac
    38341 gagcgatacg ggtcatcagc gccttacgtg ccaacaccag acgttcatcc agcagcttcc
    38401 agaaggtggc ttcatcgcct tttgcttcca gagcaatacg cggcaggttc aggctgatca
    38461 cgccgaggtt gttacgacca tcgtggatct gctcgccgtt ttcattttcc cacacgccga
    38521 ggaagctgcg gcagcccatc ggggttttaa acgaaccggt gactttcact acctgatcgt
    38581 agttcaggat atccggatac atgcgcttgc ttgcgcactc cagcgccagc tgtttgatgt
    38641 cgtagttcgg atcgcctttt ttatggttca ggccatcgcg aatcgcaaac accagtttcg
    38701 ggaacaccgc cgttttacgg tttttaccga ggccggcgat acggttacgc aggatagatt
    38761 cctgaatcag gcgcgactcc cagctggtgc ccagaccaaa accaaaggtc acaaacggtg
    38821 tttgaccgtt ggcggtgtgc agggtgttta cttcgtactc cagcgactgg aaggcatcgt
    38881 agcactcttt gatggtacga gagttagcgt agccttcggc gtccgggatg ttccactctt
    38941 ctgcggtctt gcgatgtttg ttgtagctgg cggtgacaaa cggtgccagc acttcatcga
    39001 tacggttaat ggtggtgccg ccataaatat ggctggcgac ctgagcaata atctgcgcag
    39061 ttaccgcggt tgccgtagag atcgatttcg gcggttcaat ctcggcgttc cccattttaa
    39121 agccctgggt cagcatgcct ttcaggtcga tcaacatgca gttgaacatc gggaagaacg
    39181 gtgagtaatc gagatcgtga tagtgaatat caccacgttc atgtgcctgc accacgtcac
    39241 gcggcagcag gtgctggcgt gcatagtgtt tagccacgat cccggccagc aggtcgcgct
    39301 gggttggaat caccttgctg tctttgttgg cgttttcgtt gagtaacgag gagttggtct
    39361 gctcgaccag accacggatc tcctggttca ggcgaccgcg tttttcacgt tcaatgtcgc
    39421 gatcgtgacg gtactcgatg taagcacgag ccagttgttt gtatggcccc gacatcagct
    39481 ggttttcaac tgcggtctgg atctcattga tatccacctg gttgcggccc tgcatctgct
    39541 cgctgacaac cgcagcaaca gtggcgcaat aatcggcatc atcgacttcc gctgctttag
    39601 ctgcacgcag aatcgcttct ttgatgcgct ctgatttaaa cggcactttg cagccgtctc
    39661 gtttcatcac atgcggtgtc atgatcgctc catattttta agaacaggtt atccacagaa
    39721 attgggaaag gcgttttccg gttttttcgt ttcgttttcc ggtgctttcc gcaatcgcca
    39781 tccgcttatc cacaacgccg cacctttttc gtggggagct gtagtagcaa ttatagtcga
    39841 ttaatacaac atattgggtt gggacgcatt ttaaagtcta tatatagtgc tttgcatcaa
    39901 ggatgtttga actttttttg atgtagctca aagtaaaaag cagagcgtac ggatgacggg
    39961 cgctacagcg atatgtaaat tttttaatga attttttgat tataaaatca acaaaaacaa
    40021 catactgaca ggcaaaaccc cggaatgaca ggcattaccg gggcttagcg aagatttact
    40081 tctgtaacca ccagacagcc tcaaaaggcc gtaaagtcat ggtacagggt tgtggtgagg
    40141 cttcttcgta gttatgcatc acaagctgcc agttgccgag cattttccct ggctgccagg
    40201 gttgggtctc acggctaagg ttggcaatga ccagcaaggt ttgccccttc cactcacggc
    40261 gatagcacca caatacaggg ctgtttggca gcagatcctg gtaatcgccc catgtcagga
    40321 cggcttcttg cttacgcagt gcgattaact tttggtaggt gtaaaacacc gaggactcat
    40381 cggccagcgc tgcttctacg ttgatttcct gatagttatc gcccaggcca atccacggtt
    40441 cgccagccgt aaacccggca ttatcgccgt tggtccattg catgggtgtg cgactgttgt
    40501 cacgggattt actggcgagg attgtcaata actcgtcggc atcacgccca tcgttgcgca
    40561 gctcggcaaa catattgagg ctctccacgt cgcgatagtc agtaatgcgc gtgaaatgtg
    40621 ggttggtcat gccaatctct tcgccctggt agatatacgg cgttccctgc atgccatgca
    40681 gcaccatcgc cagcattttt gccgcaggca cgcggtattc accttcatca ccaaagcgag
    40741 aaacaatgcg cggctgatcg tggttacacc agaacaaggc attccatgcc acgttgtgca
    40801 ttccttgttg ccagtggcgg aacaatgttt tcaacgccac aaagtcaggt ttagccagcg
    40861 tccatttttc accaccggga taatcgacct tcaggtgatg aaaattaaag gtcatcgaca
    40921 attcactgcc tgtcagtgcc gcgtatcgct ggcaatgctc aaggctggtg gaggacattt
    40981 cacctacggt cattaacccg cgtggcgtaa acacatcgcg gttcatctcg tgcaaaaact
    41041 cgtgtgctcg tggcccgtcg gtatagaagc gacgcccgtc gccatccagg tcttcagggt
    41101 aacgcggatc tttggagatc agattcacca catccaggcg caacccgtcg accccacgat
    41161 cggcccagaa ctcacagact tttttcagct ctgcgcgtac cgctggattc tcccagttga
    41221 gatccgcctg ttctggtgca aagagatgca aatagtactg ttcgctttct gcatgccagc
    41281 gccacgcact accaccgaat tttgaacgcc agttgttcgg tggcgtttct ggttctccat
    41341 cgcgccagat ataaaactgg cggtaagggc tttctttgtt cagcgcctcg cgaaaccagg
    41401 catgttgggt agaggtatgg ttaaacacca tatcgagaat gatacgaatc ccacgcgatt
    41461 ttgcctgtgt caccagttcg tcaaaatcgt ccagcgtgcc ataggtggga tcaatcgccg
    41521 tataattcgc tacgtcgtaa ccgttatcga cctggggaga gacataaaag ggggttagcc
    41581 agatggcatc aacgcccagt ttatgcagat agtccaggcg ttggataacg ccacgtaaat
    41641 cgccggtgcc gctacccgtg gtgtcctgaa aactctttgg ataaatctgg tagataacgc
    41701 cgttttgcca ccagtgggga agatgagtca ttacgttatt cctgcaaatg cgagggggcg
    41761 caattgcgcc ccgaagaaaa ttaaacaatg tccagcgtgc ccaggcggta tttccgctga
    41821 tagataaacg aggtgagtac aattgggatg atgatggcga tagccattgc cagcgcaaac
    41881 acctgccagt agctcggatg aatcgagaga attcccggca ggccgcctac gccgatgcca
    41941 ttcgccataa cgccgttcag gccgcatagc aatcctgcca gaccagaacc aatcatcgcg
    42001 cacagcatcg ggaagcgata tttcaggttg atgccgtaca tagcaggctc agtgactcca
    42061 agccaggcgg agatagcggc aggcacggag atctcgcgtt cattgtgctt gcggctggaa
    42121 atgataatac ctatcacggc ggagccctga gcaatattcg acagcgcaat cagcggccac
    42181 actggcgtgc cacccatgct ttgaatcatc tgcaaatcaa tagcaagcgt ggtctggtgt
    42241 acgccggtga tcaccagcgg ggcgtacagg aagccaaaca atgccgcgcc aatcggagca
    42301 aagctgccgg tcatcaggtg acgtaccgca aaggcaacgc catcgccaat catgcgacca
    42361 aacggaccaa tcagcgcatg ggcgaggaac accgcgagga tcagcgaaca gacgggcacc
    42421 accaccagat agaggtaatc cggcacgatg cgcttaagac gagtttcaat aacgcccagc
    42481 gccagtccgg ctaacagtgc cgggatgacc tgcgcctgat agcccacttt ggcgatgctg
    42541 aacatgccaa agtcccatac ttccggcagc tgctgcccga gcagataagc gttcatcagc
    42601 tgtggagaaa ccagcgttac gccaagcacg ataccgagga tcggtgtacc gcccattttt
    42661 ttcaccgctg accagcaaat cccgaccggc aggtagaaga agatcgcttc accgatcaac
    42721 cacagaaaat cgtagatcgt ttgcagggaa gggtacattt gcgccagcgt ctgaccgttg
    42781 ctcatgggca aatcgccgat cacattgcga aaaccgagga tcaaaccacc actaatcaac
    42841 gcgggcagca acgggaagaa gatctccgcg aaatgagaga tcaactgctc atgccatttc
    42901 atattctgcc gggcggcttt ttttacctgc tctttatcaa cctgcgcctg tccggttgac
    42961 gcaatcagtg cttgatagta atcacccacg ttggtgccaa tcaccacctg aaattgcccg
    43021 gcattggtga aacagccttt caccatgggg agttgctcaa tttctttcgg tctggcattg
    43081 gccggttggt tgaggacaaa gcgcaggcga gtaatacagt ggctcaccgt cgcaatattg
    43141 ccgcgcccgc cgaccagttc aatcaaccga tcgatatccg tttggtttat tttgctcatc
    43201 ataaagcccc atggcagatg acatttttgg ttggctgcag aatatattgc gcggaaaaat
    43261 ttaaaaacgg gaacgttccc gaaacgcagc gaagatcaca atttatcgtt caggaaacga
    43321 tcaggacagg gtggcgggga tgatgatttg ttgcggttcg ctgcgcccgg ttacctgcgc
    43381 gatcaactgg caggccgcct ggcgtccagc ttcggcataa ccgggatcta cggtgacgat
    43441 ctccggatgg aggaatttca ttaacggtgt attaccgacg ctcgccagtt gcaaggtgtc
    43501 gatgcgttgc tcttgcaggt atttacttgc gccaagtgcc agcgtgtcgg ttgcgcacag
    43561 taacgcggta gtttcaggcg taatcacttt tgctacgttc tcatagcctt gcttcatagc
    43621 aagccctggc agagcggcaa cgggatgcaa tttatgcgtt ttgcagaacg ccaggtaggc
    43681 ttcgtgacgt cgcttaccgg tcgtcacgtc actatgcggc acgccgagat agctgatatt
    43741 acgatgtccc tggtcataca gccgttgcat caggatcttg attgcccctt cgtcgtcata
    43801 acagaccgaa gcaaagcctt ttgcgtcacg cgccagcaga accagcgatg actgccagtg
    43861 ggctaacatt tcttctgtta tgccggtaaa accgaacagc actacgccgt cgatattacg
    43921 ccgtttcagc acccccaaat gttcggcaac taattgtggg gaaaactgac tttccatcat
    43981 gattggatcg taaccttgtt catagaacgc tggcagcatg gtttgaacgg cgagattttc
    44041 tgacaacgaa tccagacggg taacaatgat ggcgaccact ttatcgcttt gcccacgcat
    44101 agcgcgcgca gagcgggaag gggaaaatcc atgctgattc atcactgctt caacacgctc
    44161 gcgcgtgcgc tggctcacgc cgctttcgtt attcagcacc cgggaaaccg tagatttccc
    44221 cacgccgctt aagcgcgcga tgtctttgat ggtcagccga ttttgcatcc tgttgtccca
    44281 taacgtgttg tttaattatt tgagcctaac tttacctgtg cattcagcaa tgggtaaagt
    44341 ctggtttatc gttggtttag ttgccagcag gtattatatc gccataaatg ctacgaatat
    44401 tattggattc tccttattat ttgcggcgct tttttcactt accggaggtt atatggaacc
    44461 tgatcccacg cctctccctc gacggagatt aaaacttttc cggtaagccc gtcttttcac
    44521 ggcgttaccg gatgcgtaag gccgtgacgt tttaacgtcc ctgctcagct ttattacctt
    44581 caggtaaggc ttcgccacgc ctgaagacat ttctgtactg tttcagacag tgcggaggga
    44641 ctccttatgt ttaaagaaat ttttacccgg ctcattcgcc atttaccttc ccgtctggtt
    44701 catcgtgatc cattgcctgg cgcgcagcag acagtgaatg cggcggtccc gccttcttta
    44761 agtgcgcatt gcctgaaaat ggcggtgatg cccgaagaag aattgtggaa aacgttcgac
    44821 acccatccgg aagggttaaa tctggcggaa gtggaatctg cccgcgaaca acatggtgaa
    44881 aataaattac ccgcacaaca accgtcgccg tggtgggtac atttatgggt ctgctatcgc
    44941 aaccccttta atattttgct caccattctt ggtgctattt cttacgccac ggaagattta
    45001 tttgccgcag gtgttatcgc gctaatggtc gctatttcta cgttgctgaa ctttattcag
    45061 gaagctcgtt ccaccaaagc ggcagatgcc ctgaaagcga tggtcagtaa taccgcgacg
    45121 gtactgcgcg taattaacga taaaggtgaa aatggctggc tggaaatccc gatagaccaa
    45181 ctggtgcccg gcgatattat aaaactggcg gcgggggata tgatcccggc agatttacgt
    45241 atcttgcagg cgcgggatct gtttgtcgct caggcgtcgt taaccggtga gtctctgccc
    45301 gtggaaaaag ccgctaccac tcgccagccg gagcacagca atccgctgga gtgcgacacc
    45361 ctgtgtttta tgggcaccac cgtggtgagc ggcacggcac aagcaatggt gattgccaca
    45421 ggtgccaata cctggtttgg tcaactggcg gggcgtgtta gtgagcagga aagcgagccg
    45481 aatgcctttc agcaagggat tagccgcgtc agtatgctgc tgattcgctt tatgctggtg
    45541 atggctccgg tggtgttgct tatcaacggt tacaccaaag gtgactggtg ggaagcggca
    45601 ctgtttgcgc tttcggtcgc ggtaggctta acgccagaaa tgttgccgat gattgttacc
    45661 tcgacgctgg cgcgtggcgc agtcaagttg tcgaaacaga aagtgatcgt caaacatctg
    45721 gatgccattc agaactttgg cgcaatggat attctgtgca ctgataaaac cggcaccctg
    45781 acacaggata aaattgtgct ggagaatcat accgatatct ccggtaaaac tagcgaacgc
    45841 gtgctgcata gcgcgtggtt gaacagccat taccagaccg ggcttaaaaa cctgcttgat
    45901 acagcggtgc tcgaaggtac ggatgaagag tcagcgcgct cgctggccag ccgttggcaa
    45961 aaaattgatg agattccgtt tgatttcgag cgtcgtcgga tgtcggtggt tgtggcagaa
    46021 aataccgaac accatcagtt ggtttgcaaa ggcgcattgc aggaaatcct caatgtgtgt
    46081 tcgcaagtgc gtcacaatgg cgagattgta ccgctcgatg acaccatgct gcgtaagatt
    46141 aagcgggtta ctgatacgct gaatcgtcag gggctgcgcg tggttgcggt ggcgacgaaa
    46201 tacctgccag cgcgtgaagg agattaccag cgggcggatg aatccgacct gatcctcgaa
    46261 ggatatattg ctttccttga tccgccaaaa gagacaacag ctccggcact gaaggcatta
    46321 aaagcgagtg gaattaccgt aaaaatcctt actggcgaca gtgagttagt tgcagcgaaa
    46381 gtgtgccatg aagtggggct ggacgcggga gaggtggtca ttggtagtga tattgaaacg
    46441 ctatctgacg acgaactggc aaatctcgca cagcgcacca cgctgtttgc tcgtctgacg
    46501 ccgatgcata aagaacgcat cgtaacgttg ctgaagcgcg aagggcatgt ggttggcttt
    46561 atgggcgatg gtattaatga tgcgcccgcc ttacgcgctg cggatatcgg catttctgtg
    46621 gacggcgcgg tagatattgc ccgtgaagcg gctgatatca tcctgctgga aaaaagcctg
    46681 atggtgctgg aagagggggt tattgaagga cgtcgcactt tcgccaacat gctgaaatac
    46741 atcaaaatga cggcgagctc taacttcggt aatgtgttca gcgtgctggt ggcgagtgct
    46801 ttcttgccct tcctgccgat gttgccgcta cacttgctta ttcaaaatct gctgtacgat
    46861 gtgtcacagg tggcgatccc atttgataac gtcgacgacg agcaaattca aaagccgcag
    46921 cgttggaatc cggcggatct ggggcgcttt atggtcttct tcggaccgat cagctcgatt
    46981 ttcgatattc tgacgttttg cctgatgtgg tgggtattcc atgccaacac gccggaaacg
    47041 caaacgctgt tccagtcggg ctggtttgtg gtgggcttac tgtcgcaaac gctgattgtg
    47101 catatgatcc gcacccgccg tgtgccgttt attcagagct gtgcatcgtg gccgttaatg
    47161 atcatgaccg tgatcgtgat gattgtcggg atcgcattgc cgttttcacc gctggccagt
    47221 tatctgcaac tgcaggcgct gccgttaagc tatttcccgt ggctggttgc gattctggca
    47281 gggtatatga cgttaaccca gttggtgaaa ggtttctata gccgtcgtta cggttggcag
    47341 taaagaataa actgggcatg gtggcccgga atcagattgc agacaaagtg agctttgttc
    47401 atgctggatg cggcgtaacc gcctaatccg gtctacaaat tcaataaatt gcaggaatca
    47461 cgtaggcctg ataagcgtag cgcatcagag gcgattttgc ttttgtcatc agacttaatc
    47521 cgggcatgat agcccggatt tccatcaaga ttagcgacga acagcgatcg cttcgatctc
    47581 aatcttcacg tctttcggca gacgggctac ttcaacgcaa gaacgtgccg ggaaggtggc
    47641 gttgtgttcg gtgaagaagg cttcgtaggt agcattaacg gttgcgaagt cgttgagatc
    47701 tttcacaaac acggtagttt taacgatgtc gcccactttc aggccagcgg cttcgacgat
    47761 cgcttttacg ttatccagcg actgacgcgc ctgtgcagcg acatctgccg ggacttcgcc
    47821 cgttttcggg tttaccggga tctgaccgga ggtgatgatc atattgccca gatcaacgcc
    47881 ctgtacgtaa ggaccgatag ctgccggtgc attttccgtc gtgatagttt tgctcatgat
    47941 ttctccttta ttacagcggt aaaaagtctg gctcattata gggagccaga cttttattac
    48001 caaccgcaat taattggcca gcaccacatt atgagaaaac tctttttcac agtatttgca
    48061 tttgagcgcg atatcattgg cgcgttttcg cacggcaaag ctggatgaaa ccggttcggc
    48121 atggctgata cagttgctgt tcgggcagac cagcacattg tcgatgcgct ccggcagact
    48181 tgggcgcgat ttacccacca cttcatagtt gtcgatacgg ttaaccgtgg cttgcggtgc
    48241 atacaatgcc agttggtcta cttgatcttc actcaaaaag gtattttcga ttttgatcag
    48301 atctttgcgg cccatctcgc cagaaggcaa attcagacca atggtgatgc gctgatctgt
    48361 ttcggtcaac ttgaacagac tcaacagctt aaaaccgatc tgggcgggga tatggtcaat
    48421 taccgtgccg cgtttaatag cttcaacctg caatttatta tcgtgtgtca tctctatttc
    48481 cccttacagt accagatcgc gattcagaac cagtgccagt aacgcctggc gagcgaaaat
    48541 cccgttgcct gcttgctgga agtaccaggc gtgaggcgtt ttatccacat ccgtcgcaat
    48601 ctcatcaaca cgcggcagcg gatgcagcac tttcatattg gctttggcgt tgtggagatc
    48661 gctggcgcga agaacaaact gcgctttcac gttggcgtac tcggacgggt ccagacgctc
    48721 tttttgcacg cgggtcatgt acaggatgtc tacttccgcc atcacttctt caatagagct
    48781 gtgcagactc caggcgatcc ctttttcatc gagcatatcc agaatgtatt gcggcattgc
    48841 cagcgcatcc ggcgcgataa agtaaaaacg gttgctgtcg aacttcgcta gcgcctgggt
    48901 cagggagtga acggtgcggc catatttcag gtcaccaacc attgcgacgt ggagattgtc
    48961 cagacgcccc tgggtttcct gaatggtgaa taagtccagc aaggtttgcg tcggatgttg
    49021 gttggagcca tcaccggcat tcagtaccgg tacattgccg gaaaactcgg tggccaggcg
    49081 cgctgcacct tcctgcggat gacgcatcac tatcgcatcg acgtaagtgc tgataaccga
    49141 aatggtatcg gccagcgttt cgcccttttt acccagtgat gtattggcgc tgtcggagaa
    49201 gcccaccacg ctggcaccca ggcggtgcat ggaggtttcg aaagagaggc gggtgcgggt
    49261 agaggcttcg aagaaacagc tggcaataac tttgtgcttc aacagctctg gttgcgggtt
    49321 cgctttcagt ttcgccgctg tcgccagcac cagattaagg tcatcgcgac taaggtcgtt
    49381 tatggaaatg atatgtttct gatatagcgg attagccatc ttttatctcc tgacgcctgg
    49441 gcaaaaaaaa gcccctcgat tgaggggctg ggaatgggtg atcaacggga agaaaaacgg
    49501 caggccagcg tcttttttca gacgcggtaa gacaaaatgt cgaacacact gaaccataca
    49561 tcctcccggc aaattgtccg gcattatact cagctctgag agaggatcaa gaagatgatg
    49621 cagttataaa gcaaggcaaa cggttatcat gaatatatat tcaattttaa taagtaatta
    49681 atttgcttgt gcaccagggc tgtgcgcttt accacacgtg gtgcgaccca aacgatattg
    49741 ctgccagcaa tcacgccaag aatttctggc aaagcatgga aatcaaggat tcgtgctact
    49801 gcccgaccat atccggcgac ggtatggata aggataaatt caccattatg ctcgacactc
    49861 aggaccattt cagcgacgga tcgacctgcg tcaggtgtgg gaagtaattg aggattcacc
    49921 gaataaattt tttgtccttt tgtatttcta atttttatga cgcccagcaa tttcagtagt
    49981 cgtgaaacgg ttgactgact gatactgtca aagccatggt tttgcaaatc gcggcggatt
    50041 tcctcctgtg aaagataact tttttcggta atcaggcgct gacagaccgc cagttgcttc
    50101 ttttctttgg cggagtaatc atcgtattcc ttcataatgc accttttatt tattattacc
    50161 tgatgatatt tttcacattt tataatgtga gagtggttca tttatcacta caccagattt
    50221 gaaggccgct ttgataagcg gcctgtcgcg ttaaatcatc gcggcgatac tcagcacaat
    50281 catattcaac atcgtgagaa tcagcagtag cggtgctacc catctcagat aacggaccca
    50341 gggtacacgg gcaatcgcca gaccgcccat cactaccgca gaggttggcg taatcaaatt
    50401 aaccaccccg gaagcagact gataagcggt caccaccaga tcgcgttgta catgagcaaa
    50461 atcagcgagt ggagccaata ttggcatcgt taacactgca aggccggaag atgagggaac
    50521 cagaaaagag agcaatactt ccagccaata ggtcacgttg atgaaaacag tggtggaaag
    50581 ccctgaaacc agattctcag cactgtgcag aattgtgtgc gtaatcatgc cgttatccat
    50641 cacgaccaca ataccgcggg cgatgccgat aattagtgcc acaccaagga gatcccgcgc
    50701 gccgtcaata aaggtactgg tgaaggtttc ttcgcccatg cgggcaatga cacccacgat
    50761 gaccgcagct gccagaaaga cagcagagat ttccgccatc caccaaccca gtaccgctac
    50821 gccgtaaatc ataaaggcaa aggatgcagc aaaaagtatc aggatcgctt tgcgcgtggg
    50881 ggtaaattcc agcatcgttt ctgaacgatt tcccagaaaa tgcgcctggt tttcagccat
    50941 cttatccgcc acgatagagc tttccggact gttgcgaact ttgcgggcat aacgcataac
    51001 ccagtaaaca caaatcacgt agcctactaa cagcaaaata acacgcagca aaatgccctg
    51061 agtaaagggg atccctgcgg cgttggcagc aatgacggta gcaaatgggt taatggttga
    51121 gcccagcgtg ccaatgcccg atcccaataa cactgtggcg gcagcgacca gaggatcaaa
    51181 gcgggcagcc atcatgacgg gcaccagcaa cgtgtagaag gggagtgatt cttccgccat
    51241 cccataaata gtaccgccag cagcgaagag cgccatcagg atcggaatca tccactcttc
    51301 ccgaccatta aggcgagttg tcactcgttc gataccggca tctattgccc cggttttatt
    51361 tactactccc aaaaatccac cgatgatcag gacaaacagc gcgacatcaa tcgctcccgc
    51421 agtgtaggtt tgatggttgt acaagccgtc gatgggcgct aacaggacag ccgtaatacc
    51481 ctgtggttgt gcctcaacag gtgcgtaagt gcctgcaaca ggaacttcct ttcccagtgc
    51541 ggcatttgtc accatctgat attttcccgc aggtacaatc caggtcattg ccgcaactaa
    51601 cgcaatcagc acgaacaaga tggtataagc ggtgggaaat ttgaatttac ccataatgtg
    51661 atctccataa tcacgggcat acgccactcg tcgacgcatg cccgtcaggg ttagtcgccg
    51721 agagttgcta ccatcaccgc tttaatggtg tgcatccggt tttctgcttc atcgaaaaca
    51781 atagagtggg ccgattcaaa aacctcttca gtaacttcca gtcctttgag gccgtaggcc
    51841 atctcgatct cgcgtcctac tgtggtgtgc tcattatgga aggcgggcag gcagtgcata
    51901 aatttaactt cagggttacg cgtggcagta atcacctgtt gattgatctg ataaggtgcc
    51961 atcagactca ctcgttctgc ccaggcttct tttggctctc ccatcgaaac ccaaacatcc
    52021 gtatagagaa aatcaacgtc gtatacgcct tcttccacat cctctgtcag ggtgattctc
    52081 gccccagtca cgctggcgat ctcacggcat tgtgttacca gcgcttcatc tggccagaaa
    52141 gatttaggtg ccaccagacg gatatccatc cccatttttg cagcaccaac catcagcgag
    52201 ttccccatgt tatttcgggc atcaccaaga tacgcaaagc tcagctctgg cagcgttttg
    52261 cctggcgcat gttccagcat ggtcattaaa tccgcgagga tctgtgtcgg gtgaaattca
    52321 ttggtcagtc cgttccatac cggaacacca gcgaactcac ctaattcttc aacgatattc
    52381 tgaccataac cgcgatactc aataccgtca tacatgcgtc cgagtacgcg agcggtatct
    52441 ttcattgatt ctttatggcc gatttgtgag ccgctggggc cgatgtaagt cacctgcgca
    52501 ccttgatcaa aggccgcgac ttcaaaggca cagcgggtac gggtagagga tttttcaaaa
    52561 atcaacgcaa tgtttttacc gaccagcgtt tgtttttcgt ttccagattt cttggcggct
    52621 ttaagattga tagccaaatc aatcaggtat tgaatctctg ctggggtgta atcaagcagc
    52681 ttaagaaaat tacggttttt cagtgaagta gccatgggga tgtccttata aaaatggatc
    52741 aagcgtggtt atgaggggta tcaaggcgaa taagagtgcc tttgtctccg gcgagaattt
    52801 ccggaccatc tgcaagagag ccgatccctg caatgccccg gcactgacta acaaactttg
    52861 cgcaggcggt gactttcggc cccatagacc cggcatcaaa ctgcatttcg ttgagtaatt
    52921 ccggcgtgac ctgggccagc gggcgttggg ttggcttgcc ccagtcgaga tatactgcgt
    52981 ccgcatcagt gaggatcaac aaggcgtcag catggatctg acttgccagt agcgcggctg
    53041 aaagatcttt gtctataacc gcttcaatcc catggtatcc atcggctttt tcaaccaccg
    53101 gaacaccacc gccaccatta cagatcacca gatgatcgtg ggcgatcagt gtctggatgg
    53161 catcccgctc gacgatgcgt ttaggttgtg gagaaggtac gacccggcgg aaagagtgac
    53221 catctgcttt gaaaacccag cctttttctg cctgtagtac ctgagtctga gcgtggtcat
    53281 aaattgggcc gatgtactta gttgggttac tgaaggcggg atcgttggca tccacttcaa
    53341 cctgcgtcag cagcacgctg atttcacgtt gtggtagttg atttttcagc gcctgttgta
    53401 gcatgtagcc gatcatcccc tggctttcgg cccccagaat gtcgagtgga tagggagcga
    53461 catgggcata ggcgctattt tgaagtgcca gaagtccaac ttgtggacca ttcccgtgaa
    53521 ccagtacaac ccgccagtgt tgagtcagtt gggcgatagt tttagccgca agatcgatat
    53581 ttttacgttg gatttctgct tcgagtggtt cgcctcgttt aagcagggca ttaccgccga
    53641 gagcgataac cagtgttggt ttgttttcca tgatctcttc cttagatacc gtcacgatgc
    53701 aaagggcaac tcatgcaacg tgcgccgcca cgtccgcggc ctaattcatc accggggatg
    53761 ggtaaaacgg tgatgcctgc tttgtcatat ttttcgttag tccagatgtt gcgctcgtag
    53821 cccactacca ctcccggccg aagcgtgagc acattgttag cgtcattcca ctgttcgcgt
    53881 tctgcctcaa aggcatcacc ccccgtggtg attaaacgta cctggtcaat accgagggct
    53941 ttttcgatag catggaggag tgtgctttct tgagtacgtt tcaggccgcc gtgaccgtca
    54001 ggtgttagcg tccagcaatt aacatccggg cgcacaactt cgggataaac cgagaaggtg
    54061 tcgatatcga tatgagtcat aacggtgtca agatgcatac aggagcggtg tttaggcagc
    54121 tcaacagcga ttacccgttc agcctgacga tgtttaaaca atgcctgagc aagaaactcg
    54181 actccttgtg gcgtggtgcg ttcagacatg ccgatcaaca cggccccccg accaatcacc
    54241 agcacatcac caccttctaa cgtggcgtgg tcataattaa tattctcgtc gccgaaatat
    54301 ttaataaatt cgccgccagc aaattgtgga tgccagcgat aaatagcgcg tagattattg
    54361 gtttcacgtt ggcgagcagg ttttgccatt ggattaatcg atacaccgtt ataaatccag
    54421 catgaggtat cacgcgtaaa taaatgattc ggtaacggct tcataatgaa atcattaata
    54481 tcgtgggtat caaccaccat atttttaatt gaggcgggaa tttcactata agtcagccca
    54541 ccacttaaat gacgagcaag gtcccggtgt gacatctctg caagccaggt gcggacgtca
    54601 gtagcaaacg tagggccgag tcggtaatct gatatttgtg tttccagtaa ccagcttttg
    54661 gcttccggga tatccaatgt ttgtgttagt aaatcagtga gtaacagaac ttctatccct
    54721 tgctggcgca gcgtattggc gaaaatatca tgctcttcac ctgcccgctc aacggacaat
    54781 acatcgtcaa ataacaactc ctggcaattg gatggtgtta gacgtttcag actgagattt
    54841 gggcgatgca acataacgct gcgtagttgt ccaatttcag aaccaacata atgcttttcc
    54901 atcattattc ctttaacact ttttcaaaaa taaagaggag atggctgcaa acaaattaaa
    54961 tgcttgtagt caggtctgtt cag
//
LOCUS       DAZFJL010000014        50831 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFJL010000014 DAZFJL010000000
VERSION     DAZFJL010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.014"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            190..1137
                     /gene="cyoA"
                     /locus_tag="ACHJ8C_000835"
     CDS             190..1137
                     /gene="cyoA"
                     /locus_tag="ACHJ8C_000835"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO5550738.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            1159..3150
                     /gene="cyoB"
                     /locus_tag="ACHJ8C_000836"
     CDS             1159..3150
                     /gene="cyoB"
                     /locus_tag="ACHJ8C_000836"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO5550739.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            3140..3754
                     /gene="cyoC"
                     /locus_tag="ACHJ8C_000837"
     CDS             3140..3754
                     /gene="cyoC"
                     /locus_tag="ACHJ8C_000837"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO5550740.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVVSWL
                     ALTWLFGAGFIGMELYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3754..4083
                     /gene="cyoD"
                     /locus_tag="ACHJ8C_000838"
     CDS             3754..4083
                     /gene="cyoD"
                     /locus_tag="ACHJ8C_000838"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO5550741.1"
                     /translation="MSHSNASSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            4095..4985
                     /gene="cyoE"
                     /locus_tag="ACHJ8C_000839"
     CDS             4095..4985
                     /gene="cyoE"
                     /locus_tag="ACHJ8C_000839"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO5550742.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            5134..6498
                     /gene="yajR"
                     /locus_tag="ACHJ8C_000840"
     CDS             5134..6498
                     /gene="yajR"
                     /locus_tag="ACHJ8C_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5550743.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQMADAGLPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(6626..7117)
                     /gene="yajQ"
                     /locus_tag="ACHJ8C_000841"
     CDS             complement(6626..7117)
                     /gene="yajQ"
                     /locus_tag="ACHJ8C_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO5550744.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            7285..8196
                     /gene="panE"
                     /locus_tag="ACHJ8C_000842"
     CDS             7285..8196
                     /gene="panE"
                     /locus_tag="ACHJ8C_000842"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO5550745.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8159..8749
                     /gene="yajL"
                     /locus_tag="ACHJ8C_000843"
     CDS             8159..8749
                     /gene="yajL"
                     /locus_tag="ACHJ8C_000843"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO5550746.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(8803..10251)
                     /gene="thiI"
                     /locus_tag="ACHJ8C_000844"
     CDS             complement(8803..10251)
                     /gene="thiI"
                     /locus_tag="ACHJ8C_000844"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO5550747.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQSK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            10457..10699
                     /gene="xseB"
                     /locus_tag="ACHJ8C_000845"
     CDS             10457..10699
                     /gene="xseB"
                     /locus_tag="ACHJ8C_000845"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO5550748.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            10699..11598
                     /gene="ispA"
                     /locus_tag="ACHJ8C_000846"
     CDS             10699..11598
                     /gene="ispA"
                     /locus_tag="ACHJ8C_000846"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO5550749.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LTEQSLDTSALEALADYIIQRNK"
     gene            11623..13485
                     /gene="dxs"
                     /locus_tag="ACHJ8C_000847"
     CDS             11623..13485
                     /gene="dxs"
                     /locus_tag="ACHJ8C_000847"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO5550750.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNTVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDATGMEAKIKAWLA"
     gene            13540..14514
                     /gene="yajO"
                     /locus_tag="ACHJ8C_000848"
     CDS             13540..14514
                     /gene="yajO"
                     /locus_tag="ACHJ8C_000848"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO5550751.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLLRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(14568..15086)
                     /gene="pgpA"
                     /locus_tag="ACHJ8C_000849"
     CDS             complement(14568..15086)
                     /gene="pgpA"
                     /locus_tag="ACHJ8C_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO5550752.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(15064..16041)
                     /gene="thiL"
                     /locus_tag="ACHJ8C_000850"
     CDS             complement(15064..16041)
                     /gene="thiL"
                     /locus_tag="ACHJ8C_000850"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO5550753.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSNSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(16119..16538)
                     /gene="nusB"
                     /locus_tag="ACHJ8C_000851"
     CDS             complement(16119..16538)
                     /gene="nusB"
                     /locus_tag="ACHJ8C_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO5550754.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(16558..17028)
                     /gene="ribE"
                     /locus_tag="ACHJ8C_000852"
     CDS             complement(16558..17028)
                     /gene="ribE"
                     /locus_tag="ACHJ8C_000852"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO5550755.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(17117..18220)
                     /gene="ribD"
                     /locus_tag="ACHJ8C_000853"
     CDS             complement(17117..18220)
                     /gene="ribD"
                     /locus_tag="ACHJ8C_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO5550756.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQGL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGNDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(18224..18673)
                     /gene="nrdR"
                     /locus_tag="ACHJ8C_000854"
     CDS             complement(18224..18673)
                     /gene="nrdR"
                     /locus_tag="ACHJ8C_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO5550757.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            18824..19363
                     /gene="yajI"
                     /locus_tag="ACHJ8C_000855"
     CDS             18824..19363
                     /gene="yajI"
                     /locus_tag="ACHJ8C_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO5550758.1"
                     /translation="MNTNVFRQLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            19661..20545
                     /gene="tsx"
                     /locus_tag="ACHJ8C_000856"
     CDS             19661..20545
                     /gene="tsx"
                     /locus_tag="ACHJ8C_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO5550759.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQLRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKVKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGYANNGIKTRTNNSIASSHILALNYDHWHYSVVARYWHNGGQWNDDAELNFGNGN
                     FNVHSTGWGGYLVVGYNF"
     gene            complement(20583..20930)
                     /gene="yajD"
                     /locus_tag="ACHJ8C_000857"
     CDS             complement(20583..20930)
                     /gene="yajD"
                     /locus_tag="ACHJ8C_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO5550760.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(21018..21299)
                     /locus_tag="ACHJ8C_000858"
     CDS             complement(21018..21299)
                     /locus_tag="ACHJ8C_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5550761.1"
                     /translation="MYKLSGKAVEDFRGIYDYTLGKFGEEQADRYTDSLGIFLDTLSQ
                     MPEIGQDYDAIPEVKKIAFRFHTVYYVIRVDDILIARILHQLMEPRRHW"
     gene            complement(21292..21534)
                     /locus_tag="ACHJ8C_000859"
     CDS             complement(21292..21534)
                     /locus_tag="ACHJ8C_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO5550762.1"
                     /translation="MRKITSVSVGEQLDSFITRMVQSGRYGSASEVMRSALRLLEQQE
                     SRDEAVRNAVIEGLESGESSMTLRDIAAERKQKHRV"
     gene            complement(21685..22656)
                     /gene="secF"
                     /locus_tag="ACHJ8C_000860"
     CDS             complement(21685..22656)
                     /gene="secF"
                     /locus_tag="ACHJ8C_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO5550763.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(22667..24514)
                     /gene="secD"
                     /locus_tag="ACHJ8C_000861"
     CDS             complement(22667..24514)
                     /gene="secD"
                     /locus_tag="ACHJ8C_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO5550764.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPSEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(24542..24874)
                     /gene="yajC"
                     /locus_tag="ACHJ8C_000862"
     CDS             complement(24542..24874)
                     /gene="yajC"
                     /locus_tag="ACHJ8C_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO5550765.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(24897..26024)
                     /gene="tgt"
                     /locus_tag="ACHJ8C_000863"
     CDS             complement(24897..26024)
                     /gene="tgt"
                     /locus_tag="ACHJ8C_000863"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO5550766.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(26079..27149)
                     /gene="queA"
                     /locus_tag="ACHJ8C_000864"
     CDS             complement(26079..27149)
                     /gene="queA"
                     /locus_tag="ACHJ8C_000864"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO5550767.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDQRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            27242..27823
                     /gene="acpH"
                     /locus_tag="ACHJ8C_000865"
     CDS             27242..27823
                     /gene="acpH"
                     /locus_tag="ACHJ8C_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO5550768.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRNETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFT
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQSVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMEQASRKAL"
     gene            complement(27828..29645)
                     /gene="malZ"
                     /locus_tag="ACHJ8C_000866"
     CDS             complement(27828..29645)
                     /gene="malZ"
                     /locus_tag="ACHJ8C_000866"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO5550769.1"
                     /translation="MMLNAWHLPVPPFVKQSKEQLLITLWLTSEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAIDVPDIGPQWAADQIFYQIFPDRFARNLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGIRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPDAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     SALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(29801..31174)
                     /gene="proY"
                     /locus_tag="ACHJ8C_000867"
     CDS             complement(29801..31174)
                     /gene="proY"
                     /locus_tag="ACHJ8C_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO5550770.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(31250..32569)
                     /gene="brnQ"
                     /locus_tag="ACHJ8C_000868"
     CDS             complement(31250..32569)
                     /gene="brnQ"
                     /locus_tag="ACHJ8C_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO5550771.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIIWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(32976..34271)
                     /gene="phoR"
                     /locus_tag="ACHJ8C_000869"
     CDS             complement(32976..34271)
                     /gene="phoR"
                     /locus_tag="ACHJ8C_000869"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO5550772.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(34329..35018)
                     /gene="phoB"
                     /locus_tag="ACHJ8C_000870"
     CDS             complement(34329..35018)
                     /gene="phoB"
                     /locus_tag="ACHJ8C_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO5550773.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            35208..36410
                     /gene="sbcD"
                     /locus_tag="ACHJ8C_000871"
     CDS             35208..36410
                     /gene="sbcD"
                     /locus_tag="ACHJ8C_000871"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO5550774.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQAHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHTPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            36407..39550
                     /gene="sbcC"
                     /locus_tag="ACHJ8C_000872"
     CDS             36407..39550
                     /gene="sbcC"
                     /locus_tag="ACHJ8C_000872"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO5550775.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSTTLAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTLEQTQRNAALNEMRHRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSSSHPAVEAYQALEPGVNQARLLTLEKEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRMQQLTPILETLPQSDELPHSEETVALENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLTQHQQHRPGGLSLTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAVK"
     gene            39676..40860
                     /gene="araJ"
                     /locus_tag="ACHJ8C_000873"
     CDS             39676..40860
                     /gene="araJ"
                     /locus_tag="ACHJ8C_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO5550776.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(41130..42038)
                     /gene="mak"
                     /locus_tag="ACHJ8C_000874"
     CDS             complement(41130..42038)
                     /gene="mak"
                     /locus_tag="ACHJ8C_000874"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO5550777.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSAWLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            42163..43074
                     /gene="rdgC"
                     /locus_tag="ACHJ8C_000875"
     CDS             42163..43074
                     /gene="rdgC"
                     /locus_tag="ACHJ8C_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO5550778.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLMEDGGVIRAKKQNLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(43155..43235)
                     /locus_tag="ACHJ8C_000876"
     CDS             complement(43155..43235)
                     /locus_tag="ACHJ8C_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5550779.1"
                     /translation="MTQRPWSKLQHKTHNIAALRIIARCS"
     gene            complement(43232..43570)
                     /locus_tag="ACHJ8C_000877"
                     /pseudo
     CDS             complement(43232..43570)
                     /locus_tag="ACHJ8C_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(43718..44002)
                     /gene="ppnP"
                     /locus_tag="ACHJ8C_000878"
     CDS             complement(43718..44002)
                     /gene="ppnP"
                     /locus_tag="ACHJ8C_000878"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO5550780.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSLFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(44074..44751)
                     /gene="aroM"
                     /locus_tag="ACHJ8C_000879"
     CDS             complement(44074..44751)
                     /gene="aroM"
                     /locus_tag="ACHJ8C_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO5550781.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
     gene            complement(45009..45200)
                     /gene="yaiA"
                     /locus_tag="ACHJ8C_000880"
     CDS             complement(45009..45200)
                     /gene="yaiA"
                     /locus_tag="ACHJ8C_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO5550782.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(45250..45774)
                     /gene="aroL"
                     /locus_tag="ACHJ8C_000881"
     CDS             complement(45250..45774)
                     /gene="aroL"
                     /locus_tag="ACHJ8C_000881"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO5550783.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDVLYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(45957..46415)
                     /gene="yaiI"
                     /locus_tag="ACHJ8C_000882"
     CDS             complement(45957..46415)
                     /gene="yaiI"
                     /locus_tag="ACHJ8C_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO5550784.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            46535..47344
                     /gene="proC"
                     /locus_tag="ACHJ8C_000883"
     CDS             46535..47344
                     /gene="proC"
                     /locus_tag="ACHJ8C_000883"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO5550785.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(47361..48476)
                     /gene="adrA"
                     /locus_tag="ACHJ8C_000884"
                     /gene_synonym="dgcC"
     CDS             complement(47361..48476)
                     /gene="adrA"
                     /locus_tag="ACHJ8C_000884"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO5550786.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPSPGWWWLLLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(48578..48898)
                     /gene="psiF"
                     /locus_tag="ACHJ8C_000885"
     CDS             complement(48578..48898)
                     /gene="psiF"
                     /locus_tag="ACHJ8C_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO5550787.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(49017..50432)
                     /gene="phoA"
                     /locus_tag="ACHJ8C_000886"
     CDS             complement(49017..50432)
                     /gene="phoA"
                     /locus_tag="ACHJ8C_000886"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO5550788.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWQGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(50533..50793)
                     /gene="iraP"
                     /locus_tag="ACHJ8C_000887"
     CDS             complement(50533..50793)
                     /gene="iraP"
                     /locus_tag="ACHJ8C_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO5550789.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLRHPRQ"
BASE COUNT        12385 a        13544 c        12817 g        12085 t
ORIGIN      
        1 ctcccctttc gttataacgc ccttttgcaa cagcttctta aaatcaactt gatatgtttt
       61 gcaacatatg tgacctgaca gccaaatcta agtaacagga atttaatcat gtttacagta
      121 atgtaacctt cccgtaaaat gcccacacac tttaaacgcc accagatccc gtggaattga
      181 ggtcgttaaa tgagactcag gaaatacaat aaaagtttgg gatggttgtc attatttgca
      241 ggcactgtat tgctcagtgg ctgtaattct gcgctgttag atcccaaagg acagattggt
      301 ctggagcaac gttcactgat actgacggca tttggcctga tgttgattgt cgttattccc
      361 gcaatcttga tggctgttgg tttcgcctgg aagtaccgtg cgagcaataa agatgctaag
      421 tacagcccga actggtcaca ctccaataaa gtggaagctg tggtctggac ggtacctatc
      481 ttaatcatca tcttccttgc ggtactgacc tggaaaacca ctcacgctct tgagcctagc
      541 aagccgctgg cacacgacga gaagcccatt accatcgaag tggtttccat ggactggaaa
      601 tggttcttca tctacccgga acagggcatt gcaaccgtga atgaaatcgc tttcccggcg
      661 aacactccgg tgtacttcaa agtgacctcc aactccgtga tgaactcctt cttcattccg
      721 cgtctgggta gccagattta tgccatggcc ggtatgcaga ctcgcctgca tctgatcgcc
      781 aacgaacccg gtacttatga cggtatctcc gccagctaca gcggcccggg cttctcaggc
      841 atgaagttca aagctattgc aacaccggat cgcgccgcat tcgaccaatg ggtcgcaaaa
      901 gcgaaacagt cgccgaacag catgtctgac atggcagcgt tcgaaaaact ggccgcgcct
      961 agcgaataca accaggtgga atatttctcc aacgtgaaac cagacttgtt tgccgatgtg
     1021 attaacaagt ttatggctca cggtaagagc atggacatga cccagccaga aggtgagcac
     1081 agcgcacatg aaggtatgga aggcatggac atgagccacg cggaatccgc ccattaaagg
     1141 ggttgaggaa gaataaagat gttcggaaaa ttatcacttg atgcagtccc gttccatgaa
     1201 cctatcgtca tggttacgat cgctggcatt attttgggag gtctggcgct cgttggcctg
     1261 atcacttact tcggtaagtg gacctacctg tggaaagagt ggctgacctc cgttgaccat
     1321 aaacgtctcg gtatcatgta tatcatcgtg gcgattgtga tgttgctgcg tggttttgct
     1381 gacgccatta tgatgcgtag tcagcaggcc cttgcctctg cgggcgaagc gggcttcctg
     1441 ccacctcacc actacgatca gatcttcacc gcgcatggcg tgattatgat cttcttcgtg
     1501 gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc cgctgcagat cggcgcgcgt
     1561 gacgttgcgt tcccgttcct caacaactta agcttctggt ttaccgttgt tggtgtgatt
     1621 ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga ccggctggct ggcctatcca
     1681 ccgctatcgg gaatagagta cagtccggga gtcggtgtcg attactggat atggagtctc
     1741 cagctatccg gtataggtac gacgcttacc ggtatcaact tcttcgttac cattctgaag
     1801 atgcgcgcac cgggcatgac catgttcaag atgccagtat ttacctgggc atcactgtgc
     1861 gcgaacgtac tgattattgc ttccttccca attctgacgg ttaccgtcgc gttgttgacc
     1921 ctggatcgct atctgggcac ccatttcttt accaacgata tgggtggcaa catgatgatg
     1981 tatatcaacc tgatttgggc ctggggccac ccggaagttt acatcttgat cctgcctgta
     2041 ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta aacgtctgtt tggttatacc
     2101 tcgctggtat gggcaaccgt ctgtatcacc gtgctgtcgt tcatcgtctg gctgcaccac
     2161 ttctttacga tgggtgcggg cgcgaacgta aacgccttct ttggtatcac cacgatgatt
     2221 atcgccatcc cgaccggggt gaagatcttc aactggctgt tcaccatgta tcagggccgc
     2281 atcgtgttcc attctgcgat gctgtggacc atcggtttta tcgtcacctt ctcggtgggc
     2341 gggatgactg gcgtgctgct ggcagtaccg ggcgcggact tcgttctgca taacagcctg
     2401 ttcctgattg cgcacttcca taacgtgatc atcggcggcg tggtcttcgg ctgcttcgca
     2461 gggatgacct actggtggcc taaagcgttc ggtttcaaac tgaacgaaac ctggggtaaa
     2521 cgcgcgttct ggttctggat catcggcttc ttcgttgcct ttatgccact gtatgcgctg
     2581 ggcttcatgg gcatgacccg tcgtttgagc cagcagattg acccgcagtt ccacacaatg
     2641 ctgatgattg cagccagcgg tgcagtactg attgcgctgg gtattctctg cctcgttatt
     2701 cagatgtacg tttctattcg tgaccgcgac cagaaccgtg acctgactgg cgacccgtgg
     2761 ggtggccgta cgctggagtg ggcaacctct tccccgcctc cgttctataa ctttgccgtt
     2821 gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga aagagaaagg cgaagcgtac
     2881 aaaaagcctg accactatga agaaattcat atgccaaaaa acagcggtgc cggtatcgtc
     2941 attgcggctt tctccaccat cttcggtttc gccatgatct ggcatatctg gtggctggcg
     3001 attgttggct tcgcaggcat gatcatcacc tggatcgtga aaagcttcga cgaggacgtg
     3061 gattactacg tgccggtggc agaaatcgaa aaactggaaa accagcattt cgatgagatt
     3121 actaaggcag ggctgaaaaa tggcaactga tactttgacg cacgcaactg cccacgcgca
     3181 cgaacacggg caccacgatg caggcggaac caaaatcttc ggattttgga tctacctgat
     3241 gagcgactgc attctgttct ctatcctgtt tgctacctat gccgttctgg tgaacggcac
     3301 cgcaggcggc ccgacaggta aggacatttt cgaactgccg ttcgttctgg ttgaaacttt
     3361 cctgctgttg ttcagctcca tcacctacgg catggcggct atcgccatgt acaaaaacaa
     3421 caaaagccag gttgtctcct ggctggcgtt gacctggttg tttggcgccg gatttatcgg
     3481 gatggagctc tatgaattcc atcatctgat tgttaacggc atgggtccgg atcgcagcgg
     3541 cttcctgtca gcgttctttg cgctggtcgg cacgcacggt ctgcacgtca cttccggtct
     3601 tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc ggcctgacca gcactaatcg
     3661 tacccgcatc atgtgcctga gcctgttctg gcacttcctg gatgtggttt ggatctgtgt
     3721 attcactgtt gtttatctga tgggggcgat gtaatgagtc attctaacgc aagcagcggc
     3781 gcgtcccatg gcagcgtaaa aacctacatg acaggcttta tcctgtcgat cattctgacg
     3841 gtgattccgt tctggatggt gatgacagga gctgcctctc cggccgtaat tctgggaaca
     3901 atcctggcaa tggcagtggt acaggttctg gtgcatctgg tgtgcttcct gcacatgaat
     3961 accaaatcag atgaaggctg gaacatgacg gcgtttgtct tcaccgtgct aatcatcgct
     4021 atcctggttg taggctccat ctggattatg tggaacctca actacaacat gatgatgcac
     4081 taagagcggc ggttatgatg tttaagcaat acctgcaagt aacgaaacca ggcatcatct
     4141 ttggcaacct gatctcggtg attgggggat tcctgttggc ctcaaagggc agcattgatt
     4201 atcccctgtt tatctacacg ctggttgggg tgtcactggt tgtggcgtcg ggttgtgtgt
     4261 ttaacaacta catcgacagg gatatcgaca gaaagatgga aaggacgaag aatcgggtgc
     4321 tggttaaagg cctgatctca cctgctgtct cgctggtgta cgccaccttg ctgggtattg
     4381 ctggctttat gctgctgtgg tttggcgcga atccgctggc ctgctggctg ggggtgatgg
     4441 gctttgtggt ttatgtcggc gtttatagcc tgtacatgaa acgtcattct gtctacggca
     4501 cattgattgg ttcgctctcc ggcgctgcgc ctccggtgat cggttactgt gcggtaaccg
     4561 gcgagttcga tagcggcgca gcgatcctgc tggctatctt cagcctgtgg cagatgcctc
     4621 actcttatgc catagccatt ttccgcttta aggattatca ggcggcaaac attccggtac
     4681 tgccggtggt gaaaggcatt tcggtggcga agaatcacat cacgctgtat atcatcgcct
     4741 ttgccgttgc cacgctgatg ctctctcttg gcggctacgc tgggtataaa tatctggtgg
     4801 tcgccgctgc ggtgagcgtc tggtggttag gcatggctct gcgcggctat aaagttgctg
     4861 atgacagaat ctgggcgcgc aagctgttcg gtttctctat catcgccatc actgccctct
     4921 cggtgatgat gtccgttgat tttatggtgc cggactcgca tacgctgctg gctgctgtgt
     4981 ggtaacacaa cctctcagtt aaaaaggtgc tacggcacct tttttcttag cattagaatc
     5041 atatccctct cgaaatattt actaaaaaat ccgcatgttt accccattcg tttgccgctt
     5101 tacactagtc gcgaatttaa aacagaggtg gtaatgaacg attataaaat gacgccaggt
     5161 gagcggcgcg cgacctgggg tttagggacc gtattttcat tacgcatgct gggcatgttc
     5221 atggttctgc cggttctgac cacgtacggc atggctctgc aaggtgccag cgaagcatta
     5281 atcggtattg ccattggtat ttatggtctg actcaggccg tttttcagat tccgtttggc
     5341 ctgctttcag accgcattgg tcgcaaacca ttaattgtcg gtgggctggc ggtgtttgcc
     5401 gccggtagcg tgatcgctgc gctctctgac tccatctggg gaattattct gggccgggcg
     5461 ctacaaggct ccggtgcgat tgctgctgcc gttatggcgc tgctttccga tctcacgcgc
     5521 gaacaaaacc gcaccaaagc gatggcgttt atcggcgtga gctttggcat tacctttgcc
     5581 attgcgatgg tgcttggccc gatcatcact cacaaacttg ggctgcacgc gctgttctgg
     5641 atgatcgcta ttctggcaac aaccggcatt gcgttgacca tttgggttgt gcccaacagt
     5701 agcactcacg tacttaatcg tgagtccgga atggtgaaag gcagtttcag taaagtgctg
     5761 gcggaaccgc ggctgctgaa actcaacttt ggcattatgt gtctgcatat tttgctgatg
     5821 tcgacctttg ttgccctgcc cggacagatg gctgatgcgg ggttaccggc ggctgaacac
     5881 tggaaggtct acctggcaac gatgttaatc gcctttggct cggtcgtgcc tttcattatc
     5941 tacgctgaag ttaagcgcaa aatgaagcaa gtctttgtct tctgcgtcgg gttgatcgtg
     6001 gttgcggaaa ttgtgttgtg gaacgcacaa acgcagttct ggcaactggt ggtcggcgtg
     6061 cagcttttct ttgtggcgtt taatttgatg gaagcccttc tgccttcact tatcagtaaa
     6121 gaatcgccag caggttacaa aggtacggcg atgggtgttt actccaccag ccagtttctt
     6181 ggcgtggcga ttggcggttc gctgggcggc tggattgacg gcatgtttga cggtcaggga
     6241 gtatttctcg ctggcgcaat gctggccgca gtgtggctgg cagtcgccag taccatgaaa
     6301 gaaccgccgt atgtcagcag cttgcgcatt gaaatcccgg cggacattgc cgcaaacgag
     6361 gcgttaaaag tacgtttgct ggaaactgaa ggcgtcaaag aagtgttgat tgcagaagaa
     6421 gaacattcag cttatgtgaa aatcgacagc aaagtgacga atcgctttga ggttgaacag
     6481 gcaattcgtc aggcataaaa aacgaggcga attgcctgct gcgctacgct taccaggcct
     6541 acaaagttct tgagttacaa atcgttgtag gccgaattag ctgttcatgc tgcattcggc
     6601 atgaacaaaa cgcacattgt cgcgattaat cgcggaagtt tttgaactgg aacggctgac
     6661 ccagatcgcc accacgtacc atcgccatga cagcctgcaa atcatcacga gatttacccg
     6721 ttacgcggat ctcatcaccc tgaatttgcg cctgcacttt cagtttgctg tctttgatca
     6781 tcttgacgat tttcttctgg gtcgcactct caatgccctg tttcagtttc gcttccacaa
     6841 accaggtttt accgctatga acgatatttt ccggtacatc cagcgaactg ccttcaatgc
     6901 cgcgcttcag cagcttggca cgcagaatat ccagcaactg attgacctgg aaatcggact
     6961 cgctcaacac tttgatggtt ttgctggcgt cgttcagctc aaatgaggct tcaacattac
     7021 ggaagtcaaa acgggactcc acttcgcggc tcgcgttatc gaccgcgtta cgtgcttcct
     7081 gaagatcaac ttcagagaca atatcgaaag atggcatctt ttcctctccc ttcatttttg
     7141 atgcgaagca taatacctgc aaagttaaat aaccaacagt cctgcaccag gacgtatccc
     7201 ttaattgtgc atagtcgcaa cagcatgcac aattaaggga tagccttatt gaacacccgg
     7261 agtggttgcg ggtgaggagg aacaatgaaa attaccgtat tgggatgcgg tgccttaggg
     7321 caattatggc ttacagcact ttgtaaacag ggtcacgaag ttcagggctg gctgcgcgta
     7381 ccgcaacctt attgtagcgt gaatctggtt gagacagatg gttcgatatt taacgagtct
     7441 ctgaccgcca acgatcccga ttttctcgcc accagcgatc tgctcctggt gacgctgaaa
     7501 gcatggcagg tttccgatgc cgtcaaaagc ctcgcgtcca cactgcctgt aaccacgcca
     7561 atactgttaa ttcacaacgg catgggcacc atcgaagagt tgcaaaacat tcagcagcca
     7621 ttactgatgg gcaccaccac ccatgcagct cgccgcgacg gcaatgtcat tattcatgtg
     7681 gcaaacggta tcacgcatat tggtccggca cggcaacagg acggcgatta cagttatctg
     7741 gcggatattt tgcaaaccgt gttacctgac gtcgcgtggc ataacaatat tcgcgctgag
     7801 ttgtggcgca agctggcagt caactgtgtg attaatccac tgaccgccat ctggaattgc
     7861 ccgaatggtg aattacgtca tcatccgcaa gaaattatgc agatatgcga agaagtcgcg
     7921 gcagtgatcg aacgcgaagg gcatcatact tcagcagaag atttgcgtga ttacgtgatg
     7981 caggtgattg atgcaacagc ggaaaatatc tcgtcgatgt tgcaggatat ccgcgcgctg
     8041 cgccacaccg aaatcgacta tatcaatggt tttctcttac gccgcgcccg cgcgcatggg
     8101 attgccgtac cggaaaacac ccgcctgttt gaaatggtaa aaagaaagga gagtgaatat
     8161 gagcgcatcg gcactggttt gcctcgcccc tggtagtgaa gagactgaag ccgtcaccac
     8221 tatcgacctc ttggttcgcg gcggtatcaa agtcaccact gccagcgtcg ccagcgatgg
     8281 taacctggcg attacctgct cgcgcggcgt gaagctgctg gcggatgcgc cgctggttga
     8341 agtggctgat ggcgaatatg acgtgattgt gctgcctggt ggcatcaaag gcgcagagtg
     8401 ttttcgcgat agcaccctgc tggttgaaac cgttaaacag ttccaccgtt ccgggcgtat
     8461 cgtcgcggct atttgcgccg cgccagccac cgtgctggtc ccgcacgata tcttcccgat
     8521 tggcaatatg accggcttcc cgacgctgaa agacaaaata cccgccgaac aatggcagga
     8581 caagcgcgtc gtctgggatg cacgggtaaa attgctgacc agccaggggc caggtacagc
     8641 tatcgacttt ggtctgaaaa ttatcgacct gctggttggg cgcgagaaag cccatgaagt
     8701 ggcatcacaa ctggtgatgg cggcagggat ttataattat tacgagtagt gtcggatgcg
     8761 gtaaacgtcg catctgacca gatgcgacgt tacaaccaca tattacgggc gatacacctt
     8821 cacattgtta aagccctgct cgcgcagata gagcgcctgc aggcggctca tcaccccgcg
     8881 ctcacaccac aacagccagg ttttgctctg gtcgagatcg ccaaatttgg tgctcagttt
     8941 atagaacggc agagaaacca catcaatccc ttcgactttc agtggcttat cttcctgttc
     9001 atcgatagaa cggatatcga ggatcacgtc gttcgggccg aagccattga cggtttccac
     9061 ttccaccact tcctgctcgg tctgctgggc gatttcgcgg atatcaacgt tattcgcttc
     9121 ctccaccact ttatcgagaa tgctgaagtc gaacttctct tcttccgctt caatcttcga
     9181 tttaaccgct ttcaccgtcg ggcttttgga aatcacgccg caatattccg gcatcgtgcg
     9241 ggcaaagtct tcggtgccaa tctggcgagc caggttgatg atgtgctctt tgtcgtaaga
     9301 gatcagcgga cgcaggatca gcgtatcgga gacgttatca atcaggcgta ggttggtcag
     9361 cgtctggctg gacacctggc cgagcgcttc accggtaacc agcgcctgta cgccgtaacg
     9421 ttctgccact ttggacgcgg cacgcaccat catacgcttg aggataacgc ccatctgacc
     9481 gtcgtcgatt ttctcgagaa tttccccgac gaccggttcg aaattaatag cgacaaaacg
     9541 cacgcggtgg gagctgccaa agcggttcca cagataatgc gccacctgac gtacgccaat
     9601 ttcatgcgcc gcgccgccga ggttaaagaa gcagtaatgc acgcggcagc cgcgacgcat
     9661 caacatataa ctggaaacac cggagtcgaa accaccggaa atgagcgaca acacatcttc
     9721 ctgggtgccg atcgggaaac cgccaatacc ttcgtagcgg cctttaatca gcagcagacg
     9781 atcgtcttcc acttccagat ggacagtcac ttccggattg gtcagcttca cgcgcgcgga
     9841 ttcaatatgc tgatttaaac cgccgccgac gtaacgttcc acatcaatcg agctaaaatc
     9901 atgtttgccg cggcgcttca cgcgtacgca aaaggttttg ccttccagct gatcgcgata
     9961 ttgaaccaac gctttctcga aaatatcgtg catgtcggta aacggcacgt cttcgacttc
    10021 gagaatatgg tggatacccg gaatacgggt cagggcgtcg cgaatagcca gacgctggtt
    10081 ttcatctttt gcgcgaactt cgatgttatc ccagtggcgg acgacagcga gcgtctcatc
    10141 atagtgcttt aaaacgttac gaatgttccc ggtaaggatt tttataaagc gcaagcgcac
    10201 agattggctt ttgatggtga tttccgggaa caatttaatg ataaacttca tggcggcaat
    10261 ggttcgttgg caagccttaa gcgacttgta tagggaaaaa tacagcagcc cacacctgcg
    10321 gctgcatcca ggcgcggaag tataccacta acatcgcttt gctgcgcaca tcaccttacc
    10381 attgcgcgtt atttgctatt tgccctgagt ccgttaccat gacggggcga aaaatattga
    10441 gagtcagaca ttcattatgc cgaagaaaaa tgaggctccc gccagctttg aaaaggcgct
    10501 gagcgagctg gaacagattg taacccgtct ggaaagtggc gacctgccgc tggaagaggc
    10561 gctgaacgag ttcgaacgcg gcgtgcagct ggcacgtcag gggcaggcca aattacaaca
    10621 agccgaacag cgcgtacaaa ttctgctgtc tgacaatgaa gacgcctctc taaccccttt
    10681 tacaccggac aatgagtaat ggactttccg cagcaactcg aagcctgcgt taagcaggcc
    10741 aaccaggcgc tgagccgttt tatcgcccca ctgccctttc agaacactcc cgtggtcgaa
    10801 accatgcagt atggcgcatt attaggtggt aagcgcctgc gacctttcct ggtttatgcc
    10861 actggtcata tgttcggcgt tagtacaaac acactggacg cacccgctgc cgccgtagag
    10921 tgtatccacg cttactcatt aattcatgat gatttaccgg cgatggatga tgacgatctg
    10981 cgtcgcggtt tgccgacctg ccatgtgaag tttggcgaag caaacgcgat tctcgctggt
    11041 gacgctttac aaacgctggc gttctcgatt ctaagcgatg ccgatatgcc ggaagtgtca
    11101 gatcgcgaca gaatttcgat gatttctgag ctggcgagcg ccagcggtat tgccggaatg
    11161 tgcggtggtc aggcattaga tttagacgct gaaggcaaac acgtacctct ggacgcgctt
    11221 gagcgtattc atcgtcataa aaccggcgca ttgattcgcg ccgccgttcg ccttggtgca
    11281 ttaagcgccg gagataaagg acgtcgtgct ctgccagtac tcgacaagta cgcagagagc
    11341 atcggccttg ccttccaggt tcaggatgac atcctggatg tggtaggaga tactgcaacg
    11401 ttgggaaaac gccagggtgc cgaccagcaa cttggtaaaa gtacctaccc tgcacttctg
    11461 ggtcttgagc aagcccggaa gaaagcccgg gatctgatcg acgatgcccg tcagtcgctg
    11521 aaacaactga ctgaacagtc actcgatacc tcggcactgg aagcgctagc ggactacatc
    11581 atccagcgta ataaataaac aataagtatt aataggcccc tgatgagttt tgatattgcc
    11641 aaatacccga ccctggcact ggtcgactcc acccaggagt tacgactgtt gccgaaagag
    11701 agtttaccga aactctgcga cgaactgcgc cgctatttac tcgacagcgt gagccgttcc
    11761 agcgggcact tcgcctccgg gctgggcacg gtcgaactga ccgtggcgct gcactatgtc
    11821 tacaataccc cgtttgacca gttaatctgg gatgtggggc atcaggctta tccgcataaa
    11881 attttgaccg ggcgccgcga caaaatcggc accatccgtc agaaaggtgg cctgcacccg
    11941 ttcccgtggc gcggcgaaag cgaatatgac gtattaagcg tcgggcattc atcaacctcc
    12001 atcagtgccg gaattgggat cgcagttgct gccgagaaag aaggcaaaaa tcgccgcacc
    12061 gtctgtgtca ttggcgatgg cgcgattact gctggcatgg cgtttgaagc gatgaatcac
    12121 gcgggcgata tccgtcctga tatgctggtg gttctcaacg acaatgaaat gtcgatttcc
    12181 gaaaatgttg gcgcgctcaa caaccatctg gcgcagctgc tttccggtaa gctttactct
    12241 tcactgcgcg aaggcggaaa aaaagttttc tctggcgtgc cacccattaa agagctgctc
    12301 aaacgtaccg aagaacatat taaaggcatg gtagtgcctg gcacgttgtt tgaagagctg
    12361 ggctttaact acatcggccc ggtggacggt cacgatgtgc tggggcttat caccacgctt
    12421 aagaacatgc gcgacctgaa aggcccgcag ttcctgcata tcatgaccaa aaaaggtcgt
    12481 ggttatgaac cggcagaaaa agacccaatc accttccacg ccgtgcctaa atttgatccc
    12541 tccagcggtt gtctgccgaa aagtagcggc ggtttaccaa gctattcaaa aatctttggc
    12601 gactggttgt gcgaaaccgc agcgaaagat aacaagctga tggcgattac tccggcgatg
    12661 cgtgaaggtt ccggcatggt cgagttttca cgtaaattcc cggatcgcta cttcgacgtg
    12721 gcaattgccg agcaacacgc agtgaccttt gctgcgggtc tggcgattgg aggctacaaa
    12781 cccattgttg cgatctactc caccttcctg caacgcgcct atgatcaggt gctgcatgac
    12841 gtggcgattc aaaagcttcc ggtcctgttc gccatcgacc gtgcgggcat tgttggtgct
    12901 gacggtcaaa cccaccaggg cgcttttgat ctctcttacc tgcgctgcat accggaaatg
    12961 gtcattatga ccccgagcga tgaaaacgaa tgtcgccaga tgctctatac cggctatcac
    13021 tataacgatg gcccgtccgc ggtgcgctac ccgcgcggca acacagttgg cgtggaactg
    13081 acgccactgg aaaaactgcc aattggcaaa ggcattgtga agcgtcgtgg tgagaaactg
    13141 gcgatcctta actttggtac gctgatgcca gaagcggcga aagtcgctga atcgctgaac
    13201 gctacgctgg tcgatatgcg tttcgtgaaa ccgcttgatg aagcgttaat tctggaaatg
    13261 gccgccagcc atgaagcgct ggttaccgta gaagaaaacg ccattatggg tggtgcaggc
    13321 agcggcgtga acgaagtgtt gatggcccat cgtaaaccag tacccgtgct gaacattggc
    13381 ctgccggact tctttattcc acaaggaact caggaagaaa tgcgcgccga actcggcctc
    13441 gatgccaccg gtatggaagc caaaatcaaa gcctggctgg cataatccct actctactcc
    13501 tgctatgctt aagaaattat tcatggcagg agtggcagca tgcaatacaa ccccttagga
    13561 aaaaccgacc ttcgtgtttc ccgactttgc ctcggctgta tgacctttgg cgagccagat
    13621 cgcggtaatc acgcatggac actgccggaa gagagcagcc gtcccatcat taaacgtgcg
    13681 ctggaaggcg gcataaattt ctttgacacc gccaatagct attccgatgg cagcagcgaa
    13741 gagatcgtcg gtcgcgcact gcgggatttc gcccgtcgtg aagacgtggt cgttgcgacc
    13801 aaagtgttcc atcgcgttgg tgatttaccg gaaggattat cccgtgcaca aattttgcgc
    13861 tctatcgacg acagcctgct ccgtctcggc atggattatg tcgatatcct gcaaattcat
    13921 cgctgggatt acaacacgcc gatcgaagag acgctggaag ccctgaacga cgtggtaaaa
    13981 gccgggaaag cgcgttatat cggcgcgtca tccatgcacg cttcgcagtt tgctcaagca
    14041 ctggaactac aaaaacagca cggctgggcg cagtttgtca gtatgcagga tcactacaat
    14101 ctgatttatc gagaagaaga gcgcgagatg ctgccactgt gttatcagga gggcgtggcg
    14161 gtgattccat ggagcccgct ggcgcggggc cgtctgacgc gtccgtgggg agaaactacc
    14221 gcacgactgg tgtctgatga ggtggggaaa aatctctata aagaaagcga tgaaaatgac
    14281 gcgcagatcg cagagcggtt aacgggcgtc agtgaagaac tcggtgcaac acgagcacaa
    14341 gttgcgctgg cctggttatt gagtaaaccg ggcattgccg caccgattat cggaacttct
    14401 cgggaggaac agcttgatga actgctaaac gcggtggata tcactttgaa gccagaacag
    14461 attgccgaac tggaaacacc gtataaaccg catcctgtcg taggatttaa ataagagtcc
    14521 aggcctgatg agacgtgaca agcgtcacat caggcatcgg tgcacaacta cgacagaata
    14581 cccagtggcc agtgatgacc gataaaatac aggatgccag cggagatcac cccggcgaca
    14641 atatcatcta tcatgattcc catgccgcca tgtacattgc ggtcaaacca gcggatcggc
    14701 cacggtttcc acatatcgag gatacggaaa atcacaaacc cggctgcgac ccactgccag
    14761 tcattggtcg gcagcgccat gagcgtgatc cacataccga taaattcgtc ccagacaatg
    14821 ctgccatgat cgtgcacgcc catgtctttc gccgtttgat gacaaagata gacgccgata
    14881 cagatcccca gcattaccac cagcgaatag agctgccagg gcaaaaaagt catcagatac
    14941 cagaagggaa tcgccgccag cgaccccatc gtgccaggaa cgatcgggct taatccactt
    15001 ccgaatccga cagcaagcag atgccacgga ttactcattt tcaggcgact tttcgcgaca
    15061 tctttatggc gtggcaaaat ggtcatatcc tttccagtct aacgtgacag gttcgccgtc
    15121 acgaataaaa caaagccctt cgatatcggc ggtcatttgc ccgatacagg taaacggtac
    15181 acccaggtgt ccgagagcca catccagcgc gccacggttc agttccggca ccgtgaaaca
    15241 caactcgtaa tcttcaccgc cagagagcgc ccagcgcagc gcctgttccg gttcaacatg
    15301 gcgagaaagc gcatcagaaa acggcagcaa tgccaggtca atacgtgcgc cgcagtcgct
    15361 ggctttcacg atatgcccga gatcggaaat cagaccgtca gagagatcga tagctgaatt
    15421 tgccagatcg cgcagtgcct gcccctgtaa aatacgcggc gacggacgga gatgacgttt
    15481 gatcaagtag tccgcatctt tagcatcggc aacctgcaaa cggttttgca aaatcgccag
    15541 cccggcagcg ctatcgcccg gtgtaccggt cacatagatc cagtcacccg gtttcgcccc
    15601 agagcgcgtt aaggctcgtc ccatcggaac aaagccgtgg atacccaacg tcattgataa
    15661 tggcccacgc gtggtatcgc cgccaatgag ttgcatatcg taataattga gaagatcaaa
    15721 caagctgttg ctgaaggact caagccacgc ttcgtctacg tccggtaagg ttaatgccaa
    15781 cgtcagccag gccggatcgg cccccatcgc tgccagatcg cttaggttca ccgctagtgc
    15841 tttataagcc agatcagctg gatcgatatc agggaggaag tggttgcccg ccaccagcgt
    15901 atcagtgctg atcgccaggg tctgtttctc ggggatattg agaagagcgc aatcgtcgcc
    15961 gatgcccagt tcgacatcaa gacgagaact tcttacacgg tcaaaataac gggcaatcag
    16021 ggagaactcg ccacatgcca tacgttacgc ctcagcagat aaaaaagaaa aggccggcga
    16081 tcatggaacg gtcttccgtg aatctaccgg cctggatatt actttttgtt agggcgaatc
    16141 acgggtgctg ctttatcgag tacgccgttg acgaacttat ggctgtcttc tgcgccgaaa
    16201 gatttcgcca gttcgatcgc ttcgttaatg gccactttgt atggcacatc gctacgttta
    16261 gacagctcgt acagcgcaat gcgcagtact gctttttcta cctggcccag ttcttccagc
    16321 agacgggaca ggtacggctt catcagtccg tcgaggtatg cggtgttagt cgccaccccg
    16381 gccagcagct cacggaagta caggacgtca acgtctttta catcctgttc agccaggaac
    16441 tggtattcaa catcagcgat gtcgttctgg gacaactgcc aggagtagag cgcctggacg
    16501 gcacactcac gagcgcggcg acgagcagca ggtttcacgg atttcccctt actaatttca
    16561 ggccttgatg gctttcaata cattaatcat ttcaagcgcg gtcagtgcag cttctgcacc
    16621 tttgttgcca gctttggtgc cagcacgttc gatcgcttgt tcaatgcttt cagtggtcag
    16681 aaccccaaaa gcaaccggaa tttcgctgtc ctgggcaaca tgcgccaggc cgttgcttgc
    16741 accaccagcg acatattcaa agtgggcagt gccaccacga ataaccgtac ccagcgcaat
    16801 caccgcgtcg tatttaccgg ttttagccag tgcacccgcc gccagcggca gctcataggc
    16861 acctggcacc caaacaacgg taatgttttc atcttttacc tgaccgatac gtttcagtgc
    16921 gtcaattgca ccttccagca ggctgtcatt gataaagttg ttgaaacgcg cgatggtgat
    16981 ggcgacgcga gcgtccgggg tagcaacgtt agcttcaata atgttcattt tcttccttcg
    17041 ggttcgagta tggccccgca ggggggcgga ttttagcata atatttcgtg cgctgcttcc
    17101 ctttcgagcc gggagatcat gcacccacta aatgcaggca aacatccggg cctacatgac
    17161 gtatctcttt gaatttaaat tggggagcgt cggctaattt ctcaagccct ggcagcgtgc
    17221 ataatccacg ggcgtcgttg cctaatagtt taggtgcgat atagacaatc agctcatcga
    17281 ctaaacccgc ctgtagcaat gcgccagcga gcgttggccc cgcttccacc cagatgctgt
    17341 taatctgctg tttacccagt tgcatcatta gtacaaccag atccaggtga cctttatgct
    17401 ctggaatcag caaggtacgc accgtttccg gccactcacg agaatcttcc tgcgtacgcg
    17461 cgaaccaggt ttcgccgggc tgctgcacaa tgcgatgttc cggcgtcacg cgattttggc
    17521 tatcaatcac aatacgtacc ggctggcgga gattttgttg cgggtagagc ccctgagttt
    17581 gttcatccag ttcagaccaa cgcactgtta aggctggatc atccgccagc accgtggcgc
    17641 tgctggttaa aatggcatga ctttgcgcgc gcagtcgttg tacgtcgcgt cgcgcctgag
    17701 gcgaagtgat ccactggctt tcgccactcg ccatcgccgt gcgaccatca agcgatgcgc
    17761 caagttttaa ctgaatataa ggaaagccgg tgcgcatccg tttgagaaag cctttattca
    17821 attgctcagc ttcactcatc atcagcccgt ggctgacgtc aatgccagcc tgttgcagac
    17881 ggtaaagtcc acgtccagcg acctgcggat tggggtcttg cattgcagca accacgcgcg
    17941 ccacgcccgc agcaattaat gcatcacaac acggtggcgt acgaccatga tggctacagg
    18001 gttcgagtgt gacatacgcc gttgcgcctt tggctttttc gccggccatg cgcaacgcgt
    18061 gcacctcggc atgcggttca cccgctcgtt gatggtaacc ttcaccgaca atttcgccat
    18121 ctttgacaat gacgcacccg acattcgggt tgggatgcgt ggtaaaacgt cctcgttgcg
    18181 ccagctttag cgcccgcgcc atgtaatact cgtcctgcac ggcttagtcc tccaggcgcg
    18241 cgatctcttc gccaaattct ttgatatctt cgaaactgcg gtagacagag gcaaaacgga
    18301 tataggcgac tttatcgagc tttttcaatt gctccatcac cagattgcca atcatcttgc
    18361 tcggcacttc gcgctcaccg gtggcgcgca actgcgattt tatatgattg attgccattt
    18421 cgacgtcatc ggaactcacc ggacgttttt ccagcgcccg cagcattccg ctacgtaatt
    18481 tctcttcatt aaacggttca cgcacgtcgt tgctttttac aacacgcggc ataaccagct
    18541 ccgccacttc aaaggtggtg aaacgttcat tacacaccag acactgccga cggcggcgta
    18601 cggatgaacc ctcgcccacg agacgagagt caattacctt agtgtccacg gcgaaacaga
    18661 atgggcaatg catacggcgt cctgaccagg tggttaacag aaatctattt taccctgaac
    18721 tgacgataca acaaaggcgc aacgcttttg agataaagga tcgatgcttt cactgctttt
    18781 accctctacc attaggctaa ttgcgacgag aaagggacga cctatgaata ctaacgtttt
    18841 tcgacagctc ctgctgggaa gcctgttcag ccttagcgcc tgtgtgcagc aaagtgaagt
    18901 gcgacagatg aaacacagcg tcagcacgct gaaccaggag atgacgcagc tcaataaaga
    18961 aacggtcaaa atcactcagc aaaacaggct gaatgcaaaa tccagcagtg gggtttacct
    19021 tctgcctgga gcaaaaacac cagcaagact ggaaagccag atcggtactt tacgtatgtc
    19081 gctggtgaat attacgcctg atacagatgg caccaccctg acgctacgta ttcagggcga
    19141 atctaatgac cctttacccg cattcagcgg aaccgttgaa tatgggcaaa ttcagggaac
    19201 aatagacaac tttcaggaaa tcaatgtgca gaatcaattg attaacgcac ctgccagcgt
    19261 cctcgccccc agcgatgttg atattccgtt acagttaaag ggtatttctg tggatcagtt
    19321 aggctttgta cgtatccacg acattcaacc tgttatgcag taaacgtatt gcggggcgat
    19381 attgtgcgtc ccgcaacatc ttccccgtca ttttgttact ccgcttacat cacctggatt
    19441 gatagtaaaa gtttgcaaca agggtgaaag tcagtacaat ccccgcccga atgtgtgtaa
    19501 acgtgaacgc aatcgattac gtaaatgata gaactgtgaa acgaaacata tttttgtgag
    19561 caatggtttt tataataggc tcctctgtat acgaaatatt tagaaacgca atttgcgcct
    19621 ttttcactcc cgcaagggat tttcaaacag tggcatacat atgaaaaaaa cattactggc
    19681 agccggtgcg gtactggcgc tctcttcgtc ttttactgtc aacgcagctg aaaacgacaa
    19741 accgcagtat ctttccgact ggtggcacca gagcgttaac gttgtcggaa gctatcacac
    19801 ccgtttcgga ccgcagctcc gcaacgatac ctaccttgag tacgaagcat tcgctaaaaa
    19861 agactggttc gacttctatg gttatgcgga tgcgccggta ttcttcggcg gtaactccga
    19921 tgcaaaaggt atctggaacc acggttctcc gctgtttatg gaaatcgaac cgcgtttctc
    19981 cattgacaag ctgaccaata ctgaccttag cttcggtccg ttcaaagagt ggtactttgc
    20041 gaacaactac atttacgaca tgggtcgtaa taaagatggc cgccagagca cctggtacat
    20101 gggtctgggt accgacatcg atactggtct gccgatgagc ctgtccatga acgtctatgc
    20161 gaaataccag tggcagaact acggcgcagc gaacgaaaac gagtgggacg gttaccgttt
    20221 caaagtgaag tactttgtgc cgattaccga tctgtggggc ggccagctga gctacatcgg
    20281 cttcaccaac ttcgactggg gttccgacct gggcgatgac agcggttatg cgaacaacgg
    20341 tattaaaaca cgtaccaata actctattgc gtccagccat attctggcgc tgaattacga
    20401 tcactggcac tactctgtcg ttgcccgtta ctggcataac ggtggtcagt ggaatgacga
    20461 tgcagaactg aacttcggca acggcaactt caacgttcac tctaccggct ggggtggtta
    20521 tctggtagta ggttacaact tctaattgtg agaatgccgg atttcatatc cggcattttt
    20581 tatcacttct tcttgttcat catcgctttc aaatcagcga atgggttgta cttcgcttca
    20641 ccgacatctt tctgcgcatc ttcccctgcg ataacggtcg taccatactg atccgcttca
    20701 gtgtatttcg aatgctcatg atcgtggcaa tagagacaaa gtaattccca gttactgcca
    20761 tcttccgggt tattagtatg gtcgtgatca atgtggtgaa cggtaagttc acgcaggttg
    20821 gaataaacaa actcgcggga acagcgaccg cagacccacg gatagatttt taatgctttt
    20881 tcgcgatagc cgctttctaa ccgcgcatag ttttttggga ttatagccat gaaaagttac
    20941 ctggatgaaa tgtagggtta cgcgcagttt cccaagatgc ggggattttc gcaatcctga
    21001 gtcaagaaat tctcacatta ccagtgacgg cgaggttcca tcagctgatg aagaatgcgc
    21061 gcaatgagaa tgtcatcgac tcttataacg taataaacag tgtgaaacct aaatgcgatc
    21121 ttctttacct ctggaatcgc atcatagtct tgccctatct cgggcatttg agagagtgta
    21181 tccagaaaaa tgccgagaga gtcggtatag cgatccgcct gctcctcacc aaactttccc
    21241 agcgtgtagt catagatccc tctgaaatct tcaacagctt ttccgctaag cttatacacg
    21301 atgtttttgc ttcctttctg ctgcgatatc acgaagcgtc atcgaacttt caccgctctc
    21361 aagaccttca ataactgcat ttctgacagc ttcatccctg gattcttgtt gttccaatag
    21421 acgaagcgct gaacgcatca cttcactggc cgagccataa cgaccagact ggaccatccg
    21481 ggtaataaaa ctatctaatt gttcgccaac gcttactgag gtaattttgc gcataagcga
    21541 tctccatgta ttaactatta atacaaagta tatgtcagaa ctctttctac aaccaaaccg
    21601 tattcagttg catgtgaaac aggcaaaaaa aaccgcagcg caaatacgcc acggtttttt
    21661 taacagaacg gatttaaaac acccttaagg cagaattgac ggctgatccg ccccttcttt
    21721 ttccactttc tgctgcaaca tgtgttcgcg cttcataccc agtttcagag ccagcgcaga
    21781 tgccacatag atggaagatg cagtaccgat ggaaacaccg ataagcatgg tcagcgagaa
    21841 gccttccagt accggaccac cgaagaggta cagcatcagg ataaccatca aggtagtacc
    21901 ggatgtgatc aaggtacggt gcagcgtctg ggtcaaggac acgttaaaga tttcgtaagg
    21961 cgtaccgcga cggatcttgc ggaagttttc acgaatacgg tccgatacca cgatactgtc
    22021 gttaagcgag taaccgataa ccgacatcaa cgatgccaca atggtcaggt caatctcgat
    22081 atggaataac gacaaaatac ccagcgtaat gataacgtcg tgcgccagcg caataacaac
    22141 ccctgccgcc agtcgccact caaagcggaa accaacgtac acgaggatag acagcagcgc
    22201 tgccatcagc gccatcgcac cggtttgcgc aaggtctgcc cccacgctcg gaccgacgaa
    22261 ctcaatacgc ttcactgccg cattctgatt ggtggattcg ttaatcacct tcagaacctg
    22321 gctgcccagc acctgaccgc cggtttcgcc ttcagcaggc ggcatacgga ccatgatgtc
    22381 gtggctgcta ccaaagtttt gcagcatcgg ctcttcaaaa ccggcttttt gcaatgcatc
    22441 acgcattacg tcaatttcag ccggtttttc gagcgtaatt tcaataaccg taccaccggt
    22501 aaaatccagc ccccagttaa agccgcgcac gcccataata acgatagcgg cgattaacag
    22561 cagaccagag atgccgaaag cccagtagtc ccagcgcata aagtcataga ctttacggcc
    22621 gtggtttagt tgttcaacag tatattcctg tgccacatcg cactcctcag attgacagct
    22681 tcttgacgcg cttgccgcca tatagcaggt ttacgatggc acgcgtaccg acaatcgcgg
    22741 taaacatcga cgtcgccaca ccgataccgg tagtaatcgc gaacccttta attgccccgg
    22801 tacccactgc atacaggatg atgactttaa tcagcgtggt gatgttcgca tcgaagatgg
    22861 aactgaatgc gccacgataa ccttcatcaa ttgcctgttg aacagtacgt ccgttgctca
    22921 actcttcttt aatacgttcg ttgatcagta cgttcgcatc gaccgctacc gcaagggtta
    22981 agacgatacc cgcgatacct ggcatactca gcgttgcgcc tggcaacagc gacataatac
    23041 cgacaatcaa gatcaagttg gcaatcagag cactggtcgc aatcagacca aacttcttat
    23101 agaagatgat catgaacaga atagaaacca gcagaccggc aaggcaagcc tccagcccct
    23161 gctcaatgtt ctgcataccc agggttgggc caatggtacg ttcttcaaca atctgaatcg
    23221 gcgcgatcaa cgcaccggca cgcagcagca gtgaaagctg acgcgcttcg cttgggttat
    23281 tgatgccagt aatacggaag ctgttaccca gacgagactg gatgttggcg atattaatca
    23341 cctcttcctg tttcaccaga accgcacgac cgtttgcatc tttcttaccg ctgtctttgt
    23401 actccacaaa cagggttgcc atcggtttgc cgatgttgtc cttggtgaag ttagacatga
    23461 tgttaccacc cgcgctatcg agcgagatgt taacctgcgg ctggttgtat tcgtcctggc
    23521 tggaagtgga gtcggtgata tggtcaccgg tcagaattac gcgtttgtac agcacaaccg
    23581 gctgaccttc gcgggtctgt ttcacttcag agtcgcccgg tacgcgaccg gatgccgcag
    23641 cggcctgatc aacgttggtg tttaccagac ggaattccag cgttgccgtc gcacccagaa
    23701 tctctttcgc acgcgcagtg tcctgaatac ctggcagttc aacaacgata cggtcagcac
    23761 cctgacgctg aaccaccggc tccgccacgc caagttggtt tacacggtta cgcaggatat
    23821 tgatgttctg ctgcaccgca tattcacgcg cttcactcag acgagcatcg ctcattaccg
    23881 cacgcagctg gttgctgccc tggctgctaa tcaccaggtc cggatggcgc ttgctcagat
    23941 acgcaatggc ttcatcacga gctttagcat cgcggaaagt gatgctcagg ccgtagttgt
    24001 tttctttacg aacggtggta tacgggatgc ccttttcacg caggtcgctg cgcaggctat
    24061 cgatattttg ttcctggagt ttgccaagcg cggtgtccat atccacttcc atcaggaagt
    24121 gaacgccgcc acgcaggtca aggccgagct tcatcggctc agcatgaata gctgccagcc
    24181 agcgcggcgt tgccggggca aggttaagcg ccacgacgta tttgtcaccc ataacgccca
    24241 ttaatgcttc acgagcgcgc agctgggtgt cagtggagtc gaagcgcgca agaatagcgc
    24301 cctcttccag cgccacagac ttagcagtta ttttttcttc ttgtaacgtt ttctggacct
    24361 ggatcagcgt ttgctcactg gcggcgactc cgcgcgcacc agtgatctga acagccggat
    24421 cctcaccaaa caggttggga agcgcataca gcagaccgat gacaatcacc acgatcagca
    24481 tgacgtactt ccacaaagga taacggttta acacggcaat tcccttaggg aaaaatttta
    24541 attacagcgc cttcatggtg cctttcggca gaacggcagc tacgaagtca cgtttaataa
    24601 ctacttcagt ggtgtcattc agtgcgatag caatgtagcc gttttccgct actttggtta
    24661 cgcgaccgac caggccaccg ttcgtcagca cttcatcacc tttagcgatg gagtccatca
    24721 gctttttgtg ttctttggtg cgcttctgct gtggacgcag gatcatgaaa tagaaaatca
    24781 gaccgaatac caccagcatc aaaatcaaag acatcgggct accttgcgcc ggtgcacccg
    24841 ttgccgctac cgcatcagaa ataaaaaagc tcattaaatt tccctcatta ttaatattaa
    24901 tcaacgttca aaggtggtac ttctcgcccc tgacgctggt aaaaatcagt tacgaagctc
    24961 tctaatttac cctcttcaat agccttgcgt aaacccgcca tcaaacgctg gtagtaacga
    25021 aggttatgaa tggtgttgag tcgcgcgcct aatatttcgt tgcaacggtc gagatgatgc
    25081 aagtaagcgc gtgaataatt gcgacaggtg tagcaatcac actcaggatc gagtgggcca
    25141 gtatcgctct tatacttcgc attgcggatt ttcaccacgc catcggtcac gaacaaatga
    25201 ccatttcggg cgttgcgggt tggcattacg cagtcaaaca tatcgatacc gcgacgtacg
    25261 ccctcaacca ggtcttctgg tttaccaacg cccatcaggt aacgcggttt gtctgccgga
    25321 atttgcgggc atacatgctc cagaatgcgg tgcatatctg ctttcggctc acccacagcc
    25381 agaccgccga cagcgtagcc atcaaaaccg atatctacca gacctttaac agaaatatca
    25441 cgtaaatctt cgtaaacact gccctgaatg ataccaaaca gcgcattttt gtttccgaga
    25501 ctgtcaaaac gctcacggct acgcttcgcc caacgcagag acatctccat ggagcgtttt
    25561 gcgtaatccc agtcagcagg atacggcgta cactcatcaa agatcatgac gatatccgaa
    25621 ccaagatcgt actgaatctc catcgacttt tccgggtcga ggaaaatcgg gtcgccgttg
    25681 atcgggttgc ggaagtgaac gccctgttcg gtgattttac gaatatcacc aaggctgaag
    25741 acctggaagc cgccggagtc ggtaaggatt ggtcctttcc actgcataaa atcgtgcaga
    25801 tcgccgtgca gtttcatgat ttcctggccc gggcgcagcc acaggtggaa ggtgttgccg
    25861 aggataattt gcgcgccagt ggcttcaact tcttccggcg tcatcccttt tacggtgccg
    25921 taagtgccaa caggcataaa acaaggcgtt tccactacgc cacgatcaaa gaccaggcgg
    25981 ccacggcgtg cgcgaccgtc ggtggtgtcc agttcaaatt tcattttttc tccactacgt
    26041 cagaaaaaca gtccaacgtt taaaccagcg ccgcggaatt actccccaac gcgctcatta
    26101 attgcctgcg gattgtacgt gataaacatc gcatcaccgt aactaaaaaa gcgatatttc
    26161 tcttctaccg ccgctttata ggcattcatg gtgtgttgat aaccagcaaa tgccgaaacc
    26221 agcataatca gcgtcgattc cggaaggtgg aagttcgtca ccagcgcatc gaccactttg
    26281 tactggaagc ccggatagat aaagatttgg gtatcgtcaa agaacggttc aatgagatcg
    26341 tttttcgctg cctgagccgc actttccagc gaacgtactg aggtggtacc aaccgcaatc
    26401 acccggttac cgcgcgcttt cgccgccagt accgcgtcta ccacatcctg cggcacttca
    26461 gcgtattccg agtgcatgat gtgatcttca atggtgtcga cgcgcaccgg ctggaaggtg
    26521 cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc ctttggcgcg taatttttcc
    26581 agcaaaggtt cgtcaaagtg cagtccggcg gtcggtgctg ctaccgcgcc gggtttttcg
    26641 ctataaaccg tttgataaag ttcgcggtct gcatcttcgt ccggacggtc gatgtacggc
    26701 ggcagcggca tatggccgat gctgttgaga atatccagca ccgaacgttg atcattaaat
    26761 tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg cgttaatact ttcgtcatcg
    26821 cccagcagca gttctgcgcc aggttttggc gctttcgagg cgcgaatatg cgcaagaatg
    26881 cgtttgtcgt cgagcatccg ctcaaccagc acttcaatct tgccgccgct ggctttacgc
    26941 ccaaacagac gcgccgggat cacgcgggta ttattaaaaa ccagaagatc gccggggttg
    27001 agcttatcga gtaaatcggt gaaagtaccg tgcgtcagcg cgcccgtcgg cccgtccagc
    27061 gacagtaaac gacagctact gcgttcaggc atgggatagt gggcaatcag ggattcgggc
    27121 aattcaaaag agaaatcggt aacgcgcatg acactgactc agactaaaat aagaggcggg
    27181 tagtctagtg ccggggcgct ctccctgcaa cctttacccc cctttggata gaatataatc
    27241 gatgaatttt ttagctcacc tgcatttagc ccatctcgcg gaaagctcgc tttccggcaa
    27301 tttactggct gatttcgtac gcggaaatcc tgaagaaagt tttccgcccg acgtcgtggc
    27361 tggtattcat atgcatcggc gaatcgacgt attaaccgac aatctgccag aagtccgcga
    27421 agcgcgggag tggtttcgta atgaaacgcg ccgtgtcgcg cctattacgc tggatgtgat
    27481 gtgggatcac tttctgtccc gccactggtc gcagctgtcg cccgattttc cgctacagga
    27541 atttacctgt tatgcccggg agcaagtgat gactattctt ccagactcac cgccacgctt
    27601 tatcaatctg aacaactact tatggtcaga gcagtggttg gtgcgctatc gcgatatgga
    27661 tttcatccag agcgtgttaa acggcatggc aagccgtcgc ccacgtctgg atgccctgcg
    27721 tgactcctgg tacgatttag acgcccatta cgatgctctc gaaacccgct tctggcagtt
    27781 ttatccgcgg atgatggagc aggcgtcacg caaggcgtta taagacgtta attcatccac
    27841 accgtagccg aaatggcagg caaagccaga atcccgtccg tcagttgccc gtgaccttct
    27901 ttgcgttgcc attgcgcgac attaagcaac ggtgacgcgg gtagcaccac ttcacaggct
    27961 tcgccacggt tgattgccac cagtacacgc tgctgattca gcacgcggac aaataccacc
    28021 acgttatctt ccgcatacag cacctgacag ccgccacgac gcaacgcctg acttttctta
    28081 cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg aatcctgctt ttccacctgc
    28141 caggggaacg gcttacggca aaacggatcg tttttgccat ccagtcccac ttcatcaccg
    28201 taataaatgc acggtacacc aggccaggtg aacagccaga ccaccgccag gggcaggcgc
    28261 gcaacatccc gaccgagcag cgttttaaat cgcgcagtat cgtggctgtc gagctgatta
    28321 aacatacgta attgttgttg atgagaaagc cctgcgcggt aattatccat ccaggccata
    28381 caggtttggg cgtcaatttg ctgcggatcg taagagatat cggtgttggc aagaaatccc
    28441 cacaacggga atgtgaagcc gcgatagttc atggcggcat cttccacatc ggcctgtaac
    28501 cattgccgtg catcgccaaa atgttcgccg acaatatacg catccggctg ggtttctttc
    28561 gccgcttcgg tgatcccggc aacgtgctgc aaattattgc gcgccccacc cgcttccccc
    28621 agcatatgca ccacatccag ccgccagcca tccatattcc acggcgcttt cagccagtgg
    28681 cggacaatac tgtcttcccc gcgataaatt tcattcacca gactttccga ctgataatcc
    28741 agcttcggca agctggcata gcccagccag tcgagcgccg tgccatcatc actaaacgag
    28801 taccagtcac gccagggtga ttcggggttg tgacaagctc cccccgtgcc acgattgtgc
    28861 ctgtcaaacc aggcatggga atcgccactg tggttaaaca cgccgtccag caccagtcgt
    28921 attcccagct gctgcgtatt gtgtcgcaaa cgcagcaacg ccccatcacc gccaaactgc
    28981 ggatcgacat ggcgataatc ctcggtatcg tatttatgca cgctgggagc tttaaacacc
    29041 ggattgagat acagcgccgt cacgccaagt tttttcagat acggcagttt ttcacttatc
    29101 ccgtccagat cgccgccata gaacgttgat ccgccagcct gcgccgtgac cggttcatcc
    29161 cagtcacgca atatgatctc ttgtccggct gcgtgatggt aatagacatg atcctgttca
    29221 gcttcacgag gaagattacg ggcgaaacga tcagggaaga tctgataaaa aatctgatcc
    29281 gcagcccatt gtgggccgat atccggtaca tcaatggcaa actgctccag tcgtgctggc
    29341 ggcattcggc tgaagccttg cggtgtaaac caacgctggc gatcgtgcca cagcaatttg
    29401 aaactataac gccgccgagg ttgtccgctg gagagatcga tcgccgcccg ccatgcggtg
    29461 acgccaggct gcggctgact gcgctgctta tgcatcggta ctgacatttc ttcgttatcg
    29521 tgttctgtac gcagcataat gcgctgcggt gggtcttcgc tcgtcagcca cagtgtaatg
    29581 agcagttgct ctttgctttg tttaacaaat gggggcaccg gcagatgcca tgcatttaac
    29641 atcataattc ccctgtgatg aaaatctgat caccttgcca tagcctaatg caggatcact
    29701 catcatctga aggatatttg ggggaggagg acgggggcgg ataactttcg gaatagccgg
    29761 atcaggattt cctcctgaac cggcttacag aacgcaagga ttactggttt tcagccagct
    29821 gacgatcgtg gcggcgctta aacatccagc caatcaacag cacaacaatc cacgcgaaac
    29881 cgacatacag cgagatacgc gtatcagggt gataaccaat caacccgata ataaagagca
    29941 ggaaaatcaa accgccgatg gtcgttgcta ccccacccgg cactttaaat ttcagcgcct
    30001 taacttcttc tggcggcaaa cggcgacgga aggcgatttg cgacagcagg atcataatcc
    30061 acacccacac cgtggcgaag gttgccagcg aagcgatcac caggaagacg ttttccggca
    30121 tgatgtagtt cagatacacc gcaaacagca gcgcggtagt catcaccagc accgtaaccc
    30181 acggaatacc gcgacgcgag gttttgctga aaatttttgg cgcgctgccc tgctctgcca
    30241 taccgtggag catacggcct acaccaaata cgtcactgtt aattgccgac agcgaagcgg
    30301 tcagcacaac aaagttaaga atgctggcgg caaaggtaat gcccatatgc tggaacgtca
    30361 gcacaaacgg gctaccggca gtgccaacct gattccacgg gtagatagac ataatgacga
    30421 acagcgtacc gacgtagaac accagaatac gcatcggcac ggagttaatc gcacgcggaa
    30481 tcgatttctc aggatctttc gcttcaccgg cggtaatccc gataatttcg atcccaccgt
    30541 aagcaaacat caccatttgc aacgacatca ccataccaag ccagccgtta ctgaagaagc
    30601 cgccgttgct ccacaggtta tgaataccgg tcggttgccc gccgttgcca atcccccaga
    30661 tgatgatgcc gaaaccggcg acaatcatga tgatgatagt ggcgacttta aagaacgaga
    30721 accagaactc cagctcaccg aataccttca cgctcatcag gtttacggcg caaatgatca
    30781 gcaccacact cagtacccaa atccagtgcg gcaccgtcgg gaaccagaca cccatataga
    30841 taccaaaagc ggtcacatcg gcgatagcga caataaggat ttcaaagcag taggtccagc
    30901 cggtaatgta acctgccagc gggccgaggt tttcctgcgc ataacgcgag aaagagctgg
    30961 ctgccgggtt atgtaccgac atttccccca gcgcacgcat aatgatatac gccgcgatac
    31021 caccgataat ataggccaac aacacgcttg gaccggccat tttgatggca tctgccgaac
    31081 cgtaaaacag cccggtgcca attgctgaac ccagtgccat aaagcgtatg tgtcgggtac
    31141 ttagcccacg ctttagcttg ttcttacttt ccatcaattc ctaacccatc aacacaataa
    31201 aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg ttcagtgatt tagtgagcgc
    31261 tggaggtcac ctgacgacct gccgcacgat cccagataat ggccagaacc accatcacca
    31321 ctgttggcat taaccacgcc agaccttgtt cggccagcgg taaacgctgc gcccaggacg
    31381 gtaagatatc gctgaatgca gatgccttga tcccgtcgag aataccaaaa agcaggctga
    31441 taaacatcgg cggagcaatc acgcgggacg aattatgcca ccatgagcgt gtaaaactta
    31501 atacaaccag tgcgatacac ggcggataaa tggcggtcag caccggtaca gagatctgaa
    31561 tcagctggct caagccgagg ttagacacca ccatcgagaa gccgccgagg ataaacacca
    31621 gcgtacgata agagagcggt acgtactggg cgaagaattc tgcacaagca caggtcaggc
    31681 caaccgccgt gaccaggcag gcgatgaaga ttaacgccgc cagcaggaag ctaccgccgc
    31741 cgccaaaggt atgctgaacg taagcatgca ggatcgccgc accgtttgca gactgatcga
    31801 ccagcgacgc gctgtctgaa cccagacgga acagcgccag gtacagcaga gtcagaccaa
    31861 cacccgccat caggccagcc cagacggtat aacgggtcag cagacgcgct tcggtaacgc
    31921 cacgagaacg cgccgcgtta acaataacga taccaaacac cattgcgccc agcgtatcca
    31981 tggtcagata gccgttgacg aagccgttag aaaacgcagc gttttgataa gcctcagtcg
    32041 ccgtgctgat agaacccgcc ggccagataa ttgcggcaac agacaggatg accagcgcga
    32101 taattttcag cggcgcaagg aagttgccca cggtatccag cagcttgccc ggatagagcg
    32161 aaaccagaat aacgatagcg aaatagacca ggctgtaaat aaacagcggc agcgcggaat
    32221 cacccgtcag cggcgcaatg cccacttcaa aagaaacggt agctgtacgc ggcgtagcga
    32281 aaagcggccc caccgccagg taacaaactg ttgccagcag tacgccagcg actttaccaa
    32341 ttggcgtgct gagactgtca acaccgccgc caacttttgc cagcgccact accgttaata
    32401 ccggtaggcc aacggcagta atgaggaagc cgaatgccgc agtccagacg tgttcgcctg
    32461 cctgcaagcc gaccattgga gggaaaataa tgttacctgc gccgacgaac aacgcaaatg
    32521 tcataaagcc cagagcgatg atatcgcgcg atcttaattg atgggtcata aatcatactg
    32581 cctgtggatg tggtgttgta acgttaaatt tttgtgccaa tccctgggga tgatacagcg
    32641 gtatatttaa acaattttag cgggaaatac tcccaaccag gcgcggcgaa gaatagtttt
    32701 tcgatttacc gcgtaaatgc agtcagtatg acagcacctg aagcgcaatt taaacgctta
    32761 taacgtttta aagcaagaag ggatctaaaa accagaggta tatagcgata agaaatcacc
    32821 aaccagtata aaacactatt tcaatgaaaa agacatggct aatcatgcga acaaaaaatc
    32881 accaaccatt gtaaaatcta acgcccgtat ttcgactgca aagaaaataa gccagcatcg
    32941 gctggcttat ggcaataaaa gatgacaaag gcgaattaat cgctgttttt ggcaattaaa
    33001 cgttccggga taacaaaact gaaacgtgta ccttttccta ctgtactctc aatattcagg
    33061 cgactttcgt gatgattcac cgcatgtttc acgatcgcta accctaatcc gctaccaccg
    33121 gtttgccggg aacgcgcttt atcaacgcga taaaaacgct cggtcaggcg cggaatatgc
    33181 tccggtgcaa tgcccggtcc gttatcttca acactaaatt cggcaccgtg cggcactcgc
    33241 tgccagcgta cggtgatatg cgtgccttcc ggcgtatgat tcacggcgtt atagaccagg
    33301 ttcgaaatcg cactgcgtag ctgatcttcg ttgcctgaca ccttgaggcc gttatctatc
    33361 tcaaaggtaa atgtctgttt tttctgactc agagtctgag cctcgcgctc aacgacgcgc
    33421 agcatcatcg gcacatcaac cttttcattg agcaaatgcg tcggtgcggc ttctattttc
    33481 gacagcgtca gcaattgctt caccaaccct tccatccgct gggtctgctc gcgcatggtg
    33541 tgcaacgctt tttcacgtac cgcgccttcc agcggttgct catccatcat ctccaggtaa
    33601 ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca cgttggcaaa aaagttacgc
    33661 cgcgcccctt ccagttgatg catttgcgtg acatctcgcg ccaccatcag caactgtttg
    33721 tgggtataag gcatgacgcg aatctccaga tgccgcccgg tgttgagcac cagattgagc
    33781 gggcgagaga aatcacgcgt tttcagatat tgcgtaaact ccgggtaacg cagcaagtta
    33841 aggatgttct gcccgttatc ttccggccag cgcaagccaa gaatctgttg cgccagaccg
    33901 ttgcaccaga aaataccgcc ctcttccgta gtcagcacca ccgcgtcggg cagcgactcc
    33961 gcgccgctac gaaagcgttt aatcagattg cccagttcac ggcggcgttt tttatttcgt
    34021 agctgcatct ggtgtaagcc gtatagtagc ggttcccagc taccacgccc cggtggcggg
    34081 gtcatactac gatccaccca cagccaccat gaaaggcgca ataaattcca gaaatgccag
    34141 ataagcagtc ctgttaccga tgccagcaaa aaccagggca ggtaaccaaa aaatgcaccc
    34201 aggatgaaag ccgggaggca gcaaagtagc agctccagca ccagcctttt ccacgacagc
    34261 cgttccagca cgtaagatac tccagttaag aaatcataag ccctgctcta cgtccgatga
    34321 gcaaggcctt aaaagcgggt tgaaaaacga taccctgtac cgcgcacggt ctgcaccatg
    34381 cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac gaatgtggac atcgaccgta
    34441 cgatcttcca catacacgtt ggttccccag acgtggttta acagctgctc gcggctgtac
    34501 acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa attctgtcgg ccccatctcc
    34561 agcggttctt cgcccgccat cacccggtga gatgtcgggt ccagacttag cccctgcatc
    34621 tcgatcacct cttctaccgc cattggcgaa atacggcgca ttaccgcttt gattcgcgcc
    34681 accagctcct tcggagaaaa cggcttggtg atatagtcat ccgcaccggt ttcaaggccg
    34741 cgcacgcgat cttcttcttc ccctctggcg gtcaacatca ccactggaat atcccgggtc
    34801 atcgactcgc gcttgaggtg tttgatgaac tggataccgg agccgccagg taacatccag
    34861 tcgaggagaa ttaaatccgg ccagggttca ttcagttgat tcacagcact gtcataatct
    34921 tccgcttcga ccggctgaaa gccattttgt tcgagcacga agcagaccat ttcgcgaatt
    34981 ggagcttcat cttctacgac cagaatacgt ctcgccatga tttgccctgt tgtaataaat
    35041 aggttgcgat cattaatgcg acgtcattat gcgtcagatt tatgacagat ttatgaaaag
    35101 ctcgtcgcac atcctttcag gttattgatt tccgtggcgc agaaaaaagc aaatggcaca
    35161 tctgtttggg tataatcgcg cccatgcttt ttcgccaggg aaccgttatg cgcatccttc
    35221 acacctcaga ctggcatctc ggccagaact tctacagtaa aagccgcgaa gctgaacatc
    35281 aggcttttct tgactggctg ctggagacag cacaagccca tcaggtggat gcgattattg
    35341 ttgccggtga tgttttcgat accggctcgc cgcccagtta cgcccgtact ttatataacc
    35401 gttttgttgt caatttacag caaactggct gtcatctggt ggtactggca ggaaaccatg
    35461 actcggtcgc cacgctgaat gaatcgcgcg atatcatggc gttcctcaat actaccgtgg
    35521 tcgccagcgc cggacatacg ccgcaaatct tgcctcgtcg cgacgggacg ccaggcgcag
    35581 tgctgtgtcc catacctttt ttacgcccgc gtgacattat taccagccag gcggggctta
    35641 acggtattga aaaacagcag catttgctgg cggctattac tgattattat caacaacact
    35701 atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc catcatcgcc actggacatt
    35761 taacgaccgt gggtgccagt aaaagtgacg ccgtgcgtga catttatatt ggcacgctgg
    35821 acgcgtttcc ggcacaaaac tttccaccag ccgactacat cgcgctcggg catattcacc
    35881 gcgcgcagat tattggcggc atggaacatg ttcgctattg cggttccccc ataccactga
    35941 gttttgatga atgcggtaag agtaaatatg tccatctggt gacattttca aacggcaaat
    36001 tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc catggcggtg ctgaaaggcg
    36061 atctggcgtc gattaccgca cagctggaac agtggcgcga tgtatcacag gagccgcctg
    36121 tctggctgga tatcgaaatc actactgatg agtatctgca tgatattcag cgcaaaatcc
    36181 aggcattaac cgaatcattg cctgtcgaag tattgctggt acgccggagt cgtgaacagc
    36241 gcgagcgtgt gttagccagc caacagcgtg aaaccctcag cgaactcagc gtcgaagagg
    36301 tgttcaatcg ccgtctggca ctggaagaac tggatgaatc acagcagcaa cgtctgcagc
    36361 atcttttcgc cacgacgttg catagcctcg ccggagaaca cgaagcatga aaattctcag
    36421 cctgcgcctg aaaaacctga actcattaaa aggcgaatgg aagattgatt tcacccgcga
    36481 gccgttcgcc agcaacgggc tgtttgctat taccggccca accggtgcgg ggaaaaccac
    36541 cctgctggac gccatttgtc tggcgctgta tcacgaaacc ccgcgcctct ctaacgtttc
    36601 acaatcgcaa aatgatctca tgacccgcga taccgccgaa tgtctggcgg aagtggagtt
    36661 tgaagtcaaa ggtgaagcgt atcgcgcgtt ctggagccag aatcgggcgc gtaaccagcc
    36721 ggacggtaat ttgcaggtgc cacgcgtaga gctggcgcgc tgtgcagacg gcaaaattct
    36781 cgccgacaaa gtgaaagata agctggaact gacggcgacg ttaaccggac tggattacgg
    36841 gcgcttcacc cgttcgatgc tgctttcgca ggggcaattt gctgccttcc tgaatgccaa
    36901 acccaaagaa cgcgcagaat tgctcgagga gttaaccggc actgaaatct acgggcaaat
    36961 ctcggcgatg gtttttgagc agcacaaatc ggcccgcaca gagctggaga agctgcaagc
    37021 gcaggccagc ggcgtcgcgt tgctcacgcc ggaacaagtg caatcgctga cagcgagttt
    37081 gcaggtactt actgacgaag aaaaacagtt aattaccgcg cagcagcaag aacaacaatc
    37141 gctaaactgg ttaacgcgtc tggacgaatt gcagcaagaa gccagccgcc gtcaacaggc
    37201 cttgcaacag gcattagccg aagaagaaaa agcgcaacct caactggcgg cgctcagtct
    37261 ggcacaaccg gcacgaaatc ttcgtccgca ctgggaacgc atcgcagaac acagcacgac
    37321 gctggcgcat actcgccagc agattgaaga agtaaatact cgcttacaga gcacaatggc
    37381 gcttcgtgcg agcattcgcc accacgcggc gaagcagtca gcagaattac agcagcagca
    37441 acaaagcctg aatgcctggt tacaggaaca cgaccgcttc cgtcagtgga acaacgaact
    37501 ggcgggttgg cgtgcacagt tctcccaaca aaccagcgat cgcgagcatc tgcggcaatg
    37561 gcagcaacag ttaactcatg ctgagcaaaa acttaatgcg cttgcggcga tcacgttgac
    37621 gttaaccgcc gatgaagttg ctagcgccct ggcgcaacat gctgagcaac gcccactgcg
    37681 tcagcgcctg gtcgcactgc atgggcagat tgttccccaa caaaaacgtc tggcgcagtt
    37741 acaggtcgct atccagaatg tcactctgga acagacgcaa cgtaatgccg cacttaacga
    37801 aatgcgccat cgttataaag aaaaaacgca acaacttgcc gatgtgaaaa ccatttgcga
    37861 gcaggaagcg cgcattaaaa cgctggaagc ccagcgcgca cagttacagg cgggtcagcc
    37921 ttgcccactt tgtggttcca gcagccaccc ggcggtcgag gcgtatcagg cgctggaacc
    37981 gggcgttaat caggcccggc tattaacgct ggaaaaagaa gtgaaaaagc tcggcgaaga
    38041 aggtgcggcg ctacgtgggc aactggatgc attaacgaag cagcttcagc gcgatgaaaa
    38101 cgaagcgcaa agcctccgac aagatgagca agcacttact caacaatggc aagccgtcac
    38161 ggccagcctc aatatcaccc tgcagccaca ggacgatatt caaccgtggc tagatgcaca
    38221 agatgagcac gaacgccagc tgcggttact cagccaacgg catgaattac aagggcagat
    38281 tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa attgaacaac gccagcagca
    38341 actcttaacg gcattggcgg gttatgcgct gacattgcca caggaagatg aagaagagag
    38401 ctggttggcg acgcgtcagc aagaagcgca gagctggcag caacgccaga acgaattaac
    38461 cgcgctgcaa aaccgtatgc agcagctgac gccgattctg gaaacgttgc cgcaaagtga
    38521 tgaactcccg cacagcgaag aaactgtggc gctggaaaac tggcggcagg ttcatgaaca
    38581 atgtctcgca ttacacagcc agcagcagac gttacagcaa caggatgttc tggcggcgca
    38641 aagtctgcaa aaagcccagg cgcagtttga caccgcgcta caggcgagcg tctttgacga
    38701 tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca ctaacgcagc tggaacagct
    38761 caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg ctggtcactc agacagcaga
    38821 aacgctgaca cagcatcagc aacaccgacc tggcgggttg tctctcactg tgacggtgga
    38881 gcagattcag caagagttag cgcaaactca ccaaaagttg cgtgaaaaca ccacgagtca
    38941 aggcgagatt cgccagcagc tgaagcagga tgcagataac cgtcagcaac aacaaacctt
    39001 aatgcagcaa attgctcaaa tgacgcagca ggttgaggac tggggatatc tgaattcgct
    39061 aataggttcc aaagagggcg ataaattccg caaatttgcc caagggctga cgctggataa
    39121 tttagtccat ctcgctaatc agcaacttac ccggctgcac gggcgctatc tgttacagcg
    39181 caaagccagc gaggcgctgg aagttgaggt tgttgatacc tggcaggctg atgcggtacg
    39241 cgatacccgt accctttccg gcggcgaaag tttcctcgtc agtctggcgc tggcactggc
    39301 gctttcagat ctggtcagcc ataaaacgcg tattgactcg ctgttccttg atgaaggttt
    39361 tggcacactg gatagcgaaa cgctggatac cgcccttgat gcgctggatg ccctgaatgc
    39421 cagtggcaaa accatcggtg tgattagcca cgttgaagcg atgaaagagc gtattccggt
    39481 gcagatcaaa gtgaagaaga tcaatggact gggctacagc aaactggaaa gtgcgtttgc
    39541 agtgaaataa ctattcagca ggataatgaa tacagagggg cgaattatct cttggcctcg
    39601 ctggtcgtta tcctgcaagc tatcacttta ttggctacgg tgattggtag ccgttctggt
    39661 ggttgtgatg gtggtatgaa aaaagttatt ttatctttgg ctctgggcac gtttggtttg
    39721 gggatggccg aatttggcat tatgggcgtg ctcacggagc tggcgcataa cgtaggaatt
    39781 tcgattcctg ccgccgggca tatgatctcg tattatgcgc tgggggtggt ggtcggtgcg
    39841 cctataatcg cgcttttttc cagccgctac tcactcaaac atatcttgtt gtttctggtg
    39901 gcgttgtgcg tcattggcaa cgccatgttc acgctctctt cgtcttacct gatgctcgcc
    39961 attggtcggc tggtttccgg ctttccgcat ggtgcatttt ttggcgtcgg cgcgatcgtg
    40021 ttatcaaaaa ttatcaaacc cggcaaagtc accgccgccg tggcagggat ggtttccggg
    40081 atgacagtcg ccaatttgct gggcattcca ctgggaacgt atttaagtca ggaatttagt
    40141 tggcgttaca cctttttgtt gatcgctgtt tttaatattg cggtgatggc atcggtctat
    40201 ttttgggtgc cggatattcg cgacgaggcg aaaggaaaac tacgcgaaca atttcacttt
    40261 ttacgcagcc ctgccccgtg gttaattttc gccgctacga tgtttggcaa cgcaggtgtg
    40321 tttgcctggt tcagctacgt aaagccatac atgatgttta tttccggttt ttcggaaacg
    40381 gcgatgacct ttattatgat gttagttggg ctagggatgg tgctggggaa tatgctaagt
    40441 ggcaggattt caggacgtta ttcaccactg cgaattgcag cagtgactga ctttatcatt
    40501 gtactggcac tgctgatgct ctttttcttc ggcggtatga aaacaacgtc gcttattttt
    40561 gcttttattt gttgcgcggg attatttgcc ctatcagcac ctctacaaat attattactg
    40621 caaaatgcca aaggcggaga gttattaggt gccgcaggtg ggcaaatagc gtttaacctc
    40681 ggtagcgccg tcggcgcata ttgcggtggt atgatgctga cgctggggct ggcatataat
    40741 tacgtggcgc tgcccgccgc cctgctttcg tttgctgcga tgtcgtcgtt gctgctgtat
    40801 ggtcggtata agcgccagca agcggcggat agtccggtgc tggcgaaacc actggggtag
    40861 attgcagtca cggtgcacgt tgcttattgt cggatgcggc gtgaacgcct tatcggccta
    40921 cggttccggc acattttgca ggcctgataa gacgcggcaa gcgtcgcatc aggcatcgga
    40981 gcaccggggg gttgccgcca ctgtgcgcgt tatttgttgc cggatgcggc gcgagcgcct
    41041 tatccggcct accggacggc tcactttgta ggcctgataa gatgcggcaa gcgtcgcatc
    41101 aggcatgata cgccaattgc ctggtttttt tactcttgtg gccataacca tgcagcgccg
    41161 cgtacgccgc tcgaatcacc atgtttcgcc ttgcgtaccg gcgtttcaca ttctccgccg
    41221 aagacaaatt gtttaatcaa ctgtggaacc gtttgatata aacggtctac attgctcatt
    41281 ccgccgccca ggacaatcac atccggatcg agaatattca cgacatgtgc cagcgatttt
    41341 gccagccgca gctcgtagcg acgcaatgcc agttccgcta ccggatcgct ttcttctacc
    41401 aggcggataa tttcactgcc tttcagcgca tgtccgctca aacgacgata atccgtcgcg
    41461 aatcccgtgc ctgaaataaa ggtttcaata caaccttgtt taccgcaata acaagggact
    41521 tcctcgcgat aacgcagttc gtcttcgtcc atccacggta gcggattgtg tccccactcg
    41581 cctgccgtgc cattgccgcc gatatgcgcc cgcccattga atgccacgcc cgcgccgcat
    41641 cccgtgccga taatcacggc aaataccgtc tgcgctcccg ctgccgcgcc atctaccgct
    41701 tctgaaaccg ccagacagtt ggcgtcattt gccagccgca cttcccgctg caaccacgcg
    41761 cttaagtctt tatcgaatgg ctgaccgttg agccaggttg aattggcatt cttcaccacg
    41821 ccggtgtagg gcgaaatcga accaggaatg cccataccta ccgttccgcg ctgccctgtt
    41881 gcctgctccg ccatatcaac caacgtggcg atcgtttcaa tagtctgccg gtaatcatca
    41941 cgcggcgtgg gcagacgatg gcggtacaac tgctcccctg catcgcccaa tgcaatcact
    42001 tcagttttgg tgccgcctaa atcgatacct atacgcacgg tactctcctt atttttttca
    42061 atatcaatag cgtagagacg gacaaccgga ttggcaatgc aaggccgccg acaattcgtt
    42121 atcatgcccg ctaaatttaa cgacaaggcc gtggaaatta tcatgctgtg gttcaaaaat
    42181 ttaatggttt accgtcttag ccgcgagatt tcgctgcgtg cagaagagat ggaaaaacag
    42241 ctagcctcga tggcatttac cccatgcggc agccaggaca tggcgaagat gggctgggta
    42301 cctccgatgg gatcgcacag cgatgcgtta acgcacgttg ccaatggtca aattgttatc
    42361 tgtgcgcgca aagaagaaaa aatcctcccg tccccggtga ttaaacaggc gctggaagcg
    42421 aaaatcgcca aactggaagc agaacaggcg cgtaagctga agaaaaccga aaaagattcg
    42481 ctgaaagacg aagtgttgca ctctctgctg ccgcgtgctt tcagccgttt tagccagaca
    42541 atgatgtgga tcgacacggt taacggtttg attatggtgg actgcgccag cgccaaaaaa
    42601 gcggaagata cgctggcact gctgcgtaaa agcctggggt cgttaccggt tgtaccattg
    42661 agcatggaaa acccgattga gctgacgctg accgaatggg tacgttccgg cagcgcggca
    42721 cagggcttcc agctgcttga tgaagccgag ctgaaatcgt tgatggaaga tggcggcgtg
    42781 atccgcgcga agaaacaaaa tctgaccagc gaagagatca ccaaccacat tgaagccgga
    42841 aaagtggtga caaaactggc gcttgactgg cagcagcgca ttcagtttgt gatgtgcgac
    42901 gatggttcgc tcaagcgtct gaagttctgc gacgagctgc gcgatcaaaa cgaagatatc
    42961 gaccgtgaag atttcgccca gcgctttgat gccgatttca tcctgatgac tggtgaactg
    43021 gcagcgttaa ttcaaaacct gattgaagga ttaggtggcg aagcacaacg ttaattgctg
    43081 atttaccttt aatgccggat gcgacgcctg ccgcgtctta tccggcgtac gaagccacac
    43141 caggaatata atcgttagct acagcgggca ataattctta gcgcagcaat attatgcgtt
    43201 ttatgctgta acttgctcca ggggcgttgt gtcattgttt ttcctcaagc cggtgacgcg
    43261 cttcactgcg aatttttcgc gttgccccgt ttttcactaa atcacgtaac agcgaaaaat
    43321 cgggttgttc gacaaataag agtaatgatg gctcctcttt taagcacgtc gttttcacat
    43381 ccgcagccag ctgtgaatta ttaatagcca gcgaccgctc ctgaggctca gtcaatgtcg
    43441 ttaacaacac cggagtatgt gcgttgtgca atgccgtttg gcgcaaatga tcattgcgac
    43501 tttgcagcaa tgcacaccac cattcatcat ctgatttatc ttttatcgtc gccagttccg
    43561 caacaatatc tggagatctt cgctgatatt tatatcaacc ttgatgaatt atgaatatat
    43621 cggaaatcga aaaaatccca gcaaaggaaa tattattatg ggttaaagag aggacatttc
    43681 agaaaaatgc cctcccgttc aaggggaagg cgaggaatta cagatagcgg cacagataag
    43741 aggtgggttc ggcaacttgc agatgaaact cgctgtgacc gggaacatta aacagcgaac
    43801 cggcttcata cacttgccag tcggtcgcgt caggcagtaa cacattcagc gcgccactga
    43861 ttaccgtcat ctcttcaggc tcagcggtgc tgaaggtgta ttcaccttca accataacac
    43921 ccacgctggc gcgaccagtg ctgctgctgg aaaagccgat cgatttcact ttgccggaaa
    43981 agtactcatt actttgaagc ataaactggc cctgtctggt agttgaacat ctacttcact
    44041 ataggggcca gaggcgctgg ctgtcacgca aaattacatc agtaattccg cagccagccg
    44101 tgcaatcaat acgttagaga gcaagacagg aacatcgagc tgtttttgca gtaaatcgcg
    44161 atgacgctgg ttaaatccca aacaatccag catgatgaca tctgcacctt ttgccagtaa
    44221 ttctttcccg gcatcaatga ttttttgttc cgaatcatga atgggattac ccaatgaaaa
    44281 tactggcggt ttctgcaaaa tttgccattt ttgcgcctga acggtcagca actcctcaac
    44341 cggtacgata acccccacct gatgatcttc aacaatagag gaaaccagcg gaggcaatat
    44401 tcgcgacggc tcaagaaaga tcgtattacg cgcagtcata ctactaatgt ttgctgtact
    44461 cattaataaa atgacgtcat aaccctgatt atcgagcact tcaaccacac cttgcaggtc
    44521 tcgttccact ttgcgacgcg aaacatgggc cagctggttg tcatttaata atgtgagaat
    44581 tgtgtcttca cctgcttctg gcgcgtactc cgccatcact tcttcgcggc ttaacttccc
    44641 cagcaggcta tgatgggaaa tattatcttc gtcaatgtat tctgtcagga gcggcaaaac
    44701 ttcctgcata ggtacaatgc cgatggtcag gatcgccaac gacgcactca ttgttcttcc
    44761 accttacttt tatttcgtcg ttaattaccg ggcaagcgct aagcaccact gctgacactt
    44821 acctgtgcta caaatgacaa aaagcgtagc agcagacctg cacggcataa tgtaaagatt
    44881 ccaaatgatt ccagtaatgg attgttattg tttaatattc taattatttg aaaaacatga
    44941 attgtgaaaa atgtgacgca gatcccttgt ggtggcactc agaatccctt ccgagcagtc
    45001 tgatgcggtc actctttgtc agggtcctca tagcgttttt tcgcatccat cgcttcctga
    45061 gcggtcggat gttcactgat caacgagtct ggtttaggat gatcggctct gagttggtac
    45121 caggttaccg tctgtcctgg ttttcctttt tcaatcgtca ctatatatgc ttcacgagga
    45181 taaggcggtt tcgttggcat cgttcttcct tatttcacgg gatgaacgtt aagtataggc
    45241 gctcgaaaat caacaattga tcgtctgtgc cagggcactg cgaatttcag aaatcacctg
    45301 gctgggttcg tttgttgcgt cgataataat atgcgcaact tcgcgatata gcacatcgcg
    45361 ttcttccagc acttcctgaa cttcttcgct cagcggtttt cccgttaagg ttggccgtaa
    45421 atcttcttcc ggtgcagctt gcagtcggtt aaccaggact gatactggcg cacacaaata
    45481 aaccacgatc ccgttatttt gcatgaagtg acgattaaat tccgtcagaa taatgccgcc
    45541 gcctgtagcg ataacggtgg atggcgcagt taccgcttcc agcgccgccg tttctctggc
    45601 acggaatccc gcccactctt ccctttcgac gatctccgcg accgtcatat tgagctgtga
    45661 ttgcaaccac tgatcggtat caacaaaccg acggttaagc gaatcggcaa gggccattcc
    45721 gaccgttgtt ttaccacagc cccgaggccc gatcagaaaa agaggttgtg tcatcgtggg
    45781 ttttccccaa taggtcgcaa tgcggcgaaa gccggtgtca tgagaatagc gatcatacca
    45841 tcaaactagt acaatttcga ttgtaaagaa aaaattccac ttaaagtgaa aattttaata
    45901 catcccttac tataccaata aattttcaag aatgaagtgt aaataataaa ttacattcag
    45961 ccacgactac gttgcacttc cagccaccac ttctccagct ccgcggcaaa ggcctggcgg
    46021 tcacgttgtg aaaggctatc tggtcccccg gtctggatcc cactggcgcg taaggtatcc
    46081 ataaaatcgc gcatcgtcag gcgctcacga atggtcgctg gcgtgtaacg ttcgccgcgt
    46141 ggattaagcg ccgccgcgcc tttctcgatg gcttcagcag ccaaaggtat atctgcggtg
    46201 atcaccaaat cgcccgcttc acactgccgg acaatttcgt tatcggcaac gtcgaaacct
    46261 gccgcgacgc gcagcgtacg aataaatcgc gatggcggca cgcgtaaact ctggtttgct
    46321 accagtacca gcggcatctg catacgttcc gccgcgcgat acaaaatctc tttaattaca
    46381 ttgggacacg cgtcggcatc cacccaaatt gtcataaagt catcctttgt tgggtaatcc
    46441 tctattgtgt cgcgcttttg ccttccggca tagttctgtt tatgcttctg ccagcgatta
    46501 tcaaaacaat gaatttcacg gcaggagtga ggcaatggaa aagaaaatcg gttttattgg
    46561 ctgcggcaat atgggaaaag ccattctcgg cggtctgatt gccagcggtc aggtgcttcc
    46621 agggcaaatc tgggtatata ccccctcccc ggataaagtc gccgccctgc atgaccagtt
    46681 cggcatcaac gccgcagaat cggcgcaaga agtggcgcaa atcgccgaca tcatttttgc
    46741 cgccgttaaa cctggcatca tgattaaagt gcttagcgaa atcacctcca gcctgaataa
    46801 agactctctg gtcgtttcta ttgctgcagg tatcacactc gaccagcttg cccgcgcgct
    46861 gggccatgac cggaaaatta tccgcgccat gccgaacact cccgcgctgg ttaatgccgg
    46921 gatgacctcc gtaacgccaa acgcgctggt aaccccagaa gataccgctg atgtgctgaa
    46981 tattttccgc tgctttggcg aagcggaagt aattgccgag ccgatgatcc acccggtggt
    47041 cggtgtgagc ggttcttcgc cagcctacgt ctttatgttt atcgaagcga tggccgacgc
    47101 cgccgtgctg ggcgggatgc cacgcgccca ggcgtataaa tttgccgctc aggcggtaat
    47161 gggttccgca aaaatggtgc tggaaacggg agaacatcca ggggcactga aagatatggt
    47221 ctgctcaccg ggaggcacca ccattgaggc ggtacgcgta ctggaagaga aaggcttccg
    47281 tgccgcagtg atcgaagcga tgacgaagtg tatggaaaaa tcagaaaaac tcagtaaatc
    47341 ctgatgactt ttgccggacg tcaggccgcc acttcggtgc ggttacgtcc ggctttcttt
    47401 gctttgtaaa gcgccaaatc tgccgatttc aaccactcac gatagtgact catttgtggg
    47461 ttcagcggcg caacccccac actaatccgt aaagttacct gtggcgtatt cggcagacgt
    47521 aatgtattta gcccttcatg cactcgtaac atggcggtaa tggcgctctc agctggcgta
    47581 ccggacatga tcaccgcaaa ctcatcgcca ccaaaccgac caatcacatc gctaccgcgc
    47641 agggttattt gtaactgtcg ggtaagcgcc acaatcgctt catcgcccac atcatggccc
    47701 caggtatcgt tgatgctctt gaaatggtcg atatcgataa tcagtaatgt cgcatcgcgg
    47761 ttatgccgcc gacagttatc aaattcattg cgtaacatag tttcccaatg acgtcggtta
    47821 tacacgcccg tcataccgtc gcgggtactc atgacctgca acctgcgttt atgttccgcc
    47881 agtttagtcg ccgtctggta gctgacccag ccaaacagca gaggataaat gacgataatg
    47941 ggaagggaga gccaccattc cagcggcgca ctattgaacg acacggtgat gcccgtcagt
    48001 tcgagggtga caaggcagga gaccaccatc aacaccagac ccgcgacaaa cagacggggg
    48061 ccgcctgccc ccatcaaatt cagacacata atcatcaaca tcgcggtgga aggcagcacg
    48121 tttacgccca ttacgcctac ccacattccc gctaatactg catcggtttt taagttgtaa
    48181 atttcccggc taagcggatc gacggccctg ctcgctattt gccaggctaa atgcggccag
    48241 acgaacgccc agccgaccaa caacagccac caccagcccg gcgacggatg cgaaaccagc
    48301 gttgaagcaa tcggtaagaa catgccagcc aggccaaccg cgcggggtag tctgacgcga
    48361 cgggcaaagc gcagcccgga ccgctgatgt tcgttttgag gagttaaagg aggttcctcc
    48421 ccatgcgccg ccgctttttt gaaaaagttt tcatcattca ttatttttgg gaacattcag
    48481 aaacaatttt cccaaattat agagacgggg ctaaaggcag aagtatgaaa tttcgggtga
    48541 gccattttcg tagctcaccc gttagcacta tcaggtatca ggcggctttc ttgaggcagg
    48601 cactcataaa cttattacga tcatcccctt tcagagattg ttgtgttgct tgattattac
    48661 attcgcgcat cttttgctgc tgtggcgtca aacttttttc accaggcgca gatttgctgt
    48721 tcttcaggca atcactcatg taggtcttac gagcatcccc tttcaacgcc tgcgccgtcg
    48781 cctgctgatt acaagaggtc atacgctgtt gttgtggggt taaggttctc tcggcagcgc
    48841 cgacggtggt taaaaaaacc agaccgaaaa gcaaggtaac cagtaatgta attttcatcg
    48901 caccatccct cttcatgttt taaccatgag cgtatgcgcc cgtgatctgt cattaagtct
    48961 ggttgctaac agcaaaaaaa ccacccggca gcgaaaattc actgccgggc gcggttttat
    49021 ttcagcccca gggcggcttt catggtgtag aagagatcgg tctggtcggt cagtccaaca
    49081 acattggcgg catgcgggcc atacgccgca atacgcaact gactgccggt atgttcttgt
    49141 gaatcctctt cggagttccc gtaactcatc accatcactg cgccatcttt ggtatttagc
    49201 gcctgggtga ggcccggagc tttggtatct ggcgcaacta tctggctggc atgggcgtga
    49261 tcagcggtga ctatgaccaa cgtgttacca tcctttttag cgaattccag cgcccgttgt
    49321 acggcttcat cgagatcgac cgtctcgcca atttgcccac aaggattcgc agcgtgatcc
    49381 tgtttatcga ttgacgcacc ttcaacttgc aggaaaaagc ctttctcatt tttactcaac
    49441 aattcaatgg ctttgtcggt catctgcgcc agggtcggta cactgtcatt acgttgcgga
    49501 ttaggcgtac aggtgactgc gggcttatct atattaccgt ggtacgttgc tttcggtcct
    49561 tgccagcgca ctggcatatt gccgtcagca aacagtccta ataggggttt ttgctgattc
    49621 gcttccgtca ccgaattcag tgaggcagca tcgctcacca actgataacc acgcgcctgt
    49681 gcctgttcac gcagcgtttt tccctgccat tcaccggcgg ttgccgtttc agcaaaggtt
    49741 tttgcgccgc cgccaagcgt aacatcggca cgggcgttaa gcagctgttc ggtaatcgat
    49801 ccttttccgc ctttttccag agcgttaccc ggacattttt cactggtcgc actcggaccg
    49861 tagcatttgc gcgaggtcac atgcgccacc agcgcagcgg gcgtggcatc ctgcaactct
    49921 gcggtagaaa cgttaccggt cgccagacct gcggcttttg ccatttccag aatcgttggg
    49981 tgatcttttt cgtgaatatc gacgcccagc gcgccgttat aggttttgac accagttgac
    50041 caggcggttg ctgatgcagc cgagtcggtg acgtagtccg gtttgccggt ttttttattc
    50101 agcgcatagt gagtgtattg cccggtaagc ggtaaggcat cgataccttt aaaaaagccg
    50161 cccgcacctt cggcataatt gcgtgcggca gtaatttccg agtcccccat cccatcgcca
    50221 atcagcaaaa taatattttt tgcaggttta tcgctaagag aatcacgcag agcggcggtc
    50281 tgatcacccg ttaagcggcg agcaccgccg ggtgcagtaa tatcgccctg agctgcccgg
    50341 ttttccagaa caggcatttc tggtgtccgg gcttttgtca caggggtaaa cagtaacggt
    50401 aagagtgcca gtgcaatagt gctttgtttc actttatttt ctccatgtac aaatacatta
    50461 aaaaataaaa acaaagcgac tataagtctc ggccgtgaca actttatgac agctgttgaa
    50521 aagattaact ttttactgac gaggatgcct caataacttc tttacgtaat cgcgcagcag
    50581 ctccgtatcg tcgtcaggaa tgctggcatc gggctttacc tcgtacagcg ccccctctac
    50641 ctgatcaatc aaccgctgtt gatcattttg cgccatattg cgaagcattg ccgtgacgat
    50701 aatctccaaa gcttctacct gcgcgcacag ttctttcgac tcttcttctt tttgggcaag
    50761 cttaaataac aactcagcaa tgagattttt catgtctgta tttccttatc caaagtatgg
    50821 agaagttaac a
//
LOCUS       DAZFJL010000015        48354 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFJL010000015 DAZFJL010000000
VERSION     DAZFJL010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.015"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            100..1320
                     /locus_tag="ACHJ8C_000888"
     CDS             100..1320
                     /locus_tag="ACHJ8C_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO5550790.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRICVSFSGGKDSGLML
                     HLTAEIARQMGKKICVLFIDWEAQFSCTINYVQSLREFYADVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESFNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQQLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLDSMPEKTAEHYRNKIAIYLHW
                     YQKKGVEVPQTQHGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1293..1922
                     /locus_tag="ACHJ8C_000889"
     CDS             1293..1922
                     /locus_tag="ACHJ8C_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO5550791.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQFSRAWTVK"
     gene            complement(1923..3083)
                     /gene="ybdL"
                     /locus_tag="ACHJ8C_000890"
     CDS             complement(1923..3083)
                     /gene="ybdL"
                     /locus_tag="ACHJ8C_000890"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO5550792.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTRMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDVDSNITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQTDFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     IADMLHAEPEHYLALPDFYRQKRDILVNALSESRLEILACEGTYFLLVDYSAVSTLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3192..4280
                     /gene="hcxA"
                     /locus_tag="ACHJ8C_000891"
     CDS             3192..4280
                     /gene="hcxA"
                     /locus_tag="ACHJ8C_000891"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO5550793.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLLEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHALHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQVEIDKMIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4290..4487)
                     /locus_tag="ACHJ8C_000892"
     CDS             complement(4290..4487)
                     /locus_tag="ACHJ8C_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO5550794.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4600..6705)
                     /gene="cstA"
                     /locus_tag="ACHJ8C_000893"
     CDS             complement(4600..6705)
                     /gene="cstA"
                     /locus_tag="ACHJ8C_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO5550795.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6886..7299)
                     /gene="entH"
                     /locus_tag="ACHJ8C_000894"
     CDS             complement(6886..7299)
                     /gene="entH"
                     /locus_tag="ACHJ8C_000894"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO5550796.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7302..8048)
                     /gene="entA"
                     /locus_tag="ACHJ8C_000895"
     CDS             complement(7302..8048)
                     /gene="entA"
                     /locus_tag="ACHJ8C_000895"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO5550797.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8048..8905)
                     /gene="entB"
                     /locus_tag="ACHJ8C_000896"
     CDS             complement(8048..8905)
                     /gene="entB"
                     /locus_tag="ACHJ8C_000896"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO5550798.1"
                     /translation="MAIPKLQAYALPEPHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAEL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8919..10529)
                     /gene="entE"
                     /locus_tag="ACHJ8C_000897"
     CDS             complement(8919..10529)
                     /gene="entE"
                     /locus_tag="ACHJ8C_000897"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO5550799.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLASGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRTSA"
     gene            complement(10539..11714)
                     /gene="entC"
                     /locus_tag="ACHJ8C_000898"
     CDS             complement(10539..11714)
                     /gene="entC"
                     /locus_tag="ACHJ8C_000898"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO5550800.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRKRSSELHVTSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            11903..12859
                     /gene="fepB"
                     /locus_tag="ACHJ8C_000899"
     CDS             11903..12859
                     /gene="fepB"
                     /locus_tag="ACHJ8C_000899"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO5550801.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLDRLKALF"
     gene            complement(12863..14113)
                     /gene="entS"
                     /locus_tag="ACHJ8C_000900"
     CDS             complement(12863..14113)
                     /gene="entS"
                     /locus_tag="ACHJ8C_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO5550802.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSVRPGLLMLLSTLGAFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14224..15228
                     /gene="fepD"
                     /locus_tag="ACHJ8C_000901"
     CDS             14224..15228
                     /gene="fepD"
                     /locus_tag="ACHJ8C_000901"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO5550803.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLISVLTAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15225..16217
                     /gene="fepG"
                     /locus_tag="ACHJ8C_000902"
     CDS             15225..16217
                     /gene="fepG"
                     /locus_tag="ACHJ8C_000902"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO5550804.1"
                     /translation="MIYVSRRLIITCLLLLIACVMAGVWGLRSGAVTLETSQVFAALM
                     GDTPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMAGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16214..17029
                     /gene="fepC"
                     /locus_tag="ACHJ8C_000903"
     CDS             16214..17029
                     /gene="fepC"
                     /locus_tag="ACHJ8C_000903"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO5550805.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEDAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTAKI"
     gene            complement(17026..18138)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHJ8C_000904"
                     /pseudo
     CDS             complement(17026..18138)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHJ8C_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
     gene            complement(18385..22266)
                     /gene="entF"
                     /locus_tag="ACHJ8C_000905"
     CDS             complement(18385..22266)
                     /gene="entF"
                     /locus_tag="ACHJ8C_000905"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO5550806.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDAQTFELPEIIDLRTNIDPHGTARALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAANPSLLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADTRYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDIPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQHFF
                     ADYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAEVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQALGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSTQRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTSANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMTPEQAWAPWIAEL
                     DIYRQDCAHVDIISPEYFKEIGPLINTQINN"
     gene            complement(22263..22481)
                     /gene="ybdZ"
                     /locus_tag="ACHJ8C_000906"
     CDS             complement(22263..22481)
                     /gene="ybdZ"
                     /locus_tag="ACHJ8C_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO5550807.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQASCQQWLEAHWRTLTPANFIQLQEAQ"
     gene            complement(22484..23686)
                     /gene="fes"
                     /locus_tag="ACHJ8C_000907"
     CDS             complement(22484..23686)
                     /gene="fes"
                     /locus_tag="ACHJ8C_000907"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO5550808.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPRSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPALTSL
                     THRRQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPQVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGHQEGMLLEQLNTGEVSAEG
                     LRIVLEAGVREPMIMRANQALYAQLHPLKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            23929..26169
                     /gene="fepA"
                     /locus_tag="ACHJ8C_000908"
     CDS             23929..26169
                     /gene="fepA"
                     /locus_tag="ACHJ8C_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO5550809.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKAAQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            26344..26964
                     /gene="entD"
                     /locus_tag="ACHJ8C_000909"
     CDS             26344..26964
                     /gene="entD"
                     /locus_tag="ACHJ8C_000909"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO5550810.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPASFREQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAGVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSAQTARELTDNIITPAEHKRLADCGLAFPLALTLAFSAKESAFKASEI
                     QTDADFLDYQIISRNKQQVIIHRENEMFAVHWQIKEKIIITLCQHD"
     gene            complement(27086..27238)
                     /gene="hokE"
                     /locus_tag="ACHJ8C_000910"
     CDS             complement(27086..27238)
                     /gene="hokE"
                     /locus_tag="ACHJ8C_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO5550811.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            27207..27476
                     /locus_tag="ACHJ8C_000911"
     CDS             27207..27476
                     /locus_tag="ACHJ8C_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5550812.1"
                     /translation="MTATRAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     PSRPQSKPETGKLQAPAVILPFFSKIAVYLYSKSCFGLLTPHLIN"
     gene            27692..28810
                     /locus_tag="ACHJ8C_000912"
     CDS             27692..28810
                     /locus_tag="ACHJ8C_000912"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO5550813.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNQ
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLMYKFNRFQACRYGLEGVITDPHTGDRRSLTEATLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGE"
     gene            28876..29124
                     /locus_tag="ACHJ8C_000913"
     CDS             28876..29124
                     /locus_tag="ACHJ8C_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO5550814.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTVLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            29189..29572
                     /locus_tag="ACHJ8C_000914"
     CDS             29189..29572
                     /locus_tag="ACHJ8C_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO5550815.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELCGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGDEISEALLHDLIND
                     SWNLVIDGLAKRDQKRLRPDSTVNQ"
     gene            29651..30304
                     /gene="nfsB"
                     /locus_tag="ACHJ8C_000915"
     CDS             29651..30304
                     /gene="nfsB"
                     /locus_tag="ACHJ8C_000915"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO5550816.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAADNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVIDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDEEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            30412..31659
                     /gene="ybdG"
                     /locus_tag="ACHJ8C_000916"
     CDS             30412..31659
                     /gene="ybdG"
                     /locus_tag="ACHJ8C_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO5550817.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(31817..33199)
                     /gene="pheP"
                     /locus_tag="ACHJ8C_000917"
     CDS             complement(31817..33199)
                     /gene="pheP"
                     /locus_tag="ACHJ8C_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO5550818.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRKVN"
     gene            complement(33301..36444)
                     /gene="cusA"
                     /locus_tag="ACHJ8C_000918"
     CDS             complement(33301..36444)
                     /gene="cusA"
                     /locus_tag="ACHJ8C_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO5550819.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDTMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(36456..37679)
                     /gene="cusB"
                     /locus_tag="ACHJ8C_000919"
     CDS             complement(36456..37679)
                     /gene="cusB"
                     /locus_tag="ACHJ8C_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO5550820.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKEEPPAEKTSTAERKVLF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFSQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(37695..38027)
                     /gene="cusF"
                     /locus_tag="ACHJ8C_000920"
     CDS             complement(37695..38027)
                     /gene="cusF"
                     /locus_tag="ACHJ8C_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO5550821.1"
                     /translation="MKKTLQVAMFSLFTVIGFNAQANEHHHETMSEAQPKVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGSLSL
                     LQDIKVSQ"
     gene            complement(38051..39433)
                     /gene="cusC"
                     /locus_tag="ACHJ8C_000921"
     CDS             complement(38051..39433)
                     /gene="cusC"
                     /locus_tag="ACHJ8C_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO5550822.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMAALKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPVFNAGRNQANLDIAEIRQQQSVVNYEQKVQNAFKEVADALALRQTLNAQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            39590..40273
                     /gene="cusR"
                     /locus_tag="ACHJ8C_000922"
     CDS             39590..40273
                     /gene="cusR"
                     /locus_tag="ACHJ8C_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO5550823.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            40263..41705
                     /gene="cusS"
                     /locus_tag="ACHJ8C_000923"
     CDS             40263..41705
                     /gene="cusS"
                     /locus_tag="ACHJ8C_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO5550824.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     TGETIVVRCQTVDHLVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIILPA"
     gene            41855..44092
                     /gene="nrfB"
                     /locus_tag="ACHJ8C_000924"
     CDS             41855..44092
                     /gene="nrfB"
                     /locus_tag="ACHJ8C_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO5550825.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMINRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQISSSLIAEMPASVA
                     LHYAVLPLRLDNDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            44079..47051
                     /gene="nfrA"
                     /locus_tag="ACHJ8C_000925"
     CDS             44079..47051
                     /gene="nfrA"
                     /locus_tag="ACHJ8C_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO5550826.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDDRLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAAQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     TPLFTTDAQEKSWLYLLSKYSGNPVQALANYTVQFADNRQYVVGATLPVLLKEGQYGA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHGMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRNQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYVRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            47052..47942
                     /locus_tag="ACHJ8C_000926"
     CDS             47052..47942
                     /locus_tag="ACHJ8C_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO5550827.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKTTGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVARKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPAAQSILEY"
BASE COUNT        11594 a        13315 c        12352 g        11093 t
ORIGIN      
        1 tgtgaatata aaataatgtg ctctatcttg cttcgtttat cctataatta gcggattcat
       61 ctcacgaatc accccgtaac gttcagagag cgtttttaga tgtctattta taaaatcccg
      121 cttccactga atattctgga agctgcaaaa gaacgtatta cctggacact taatacccta
      181 ccccgtatct gtgtttcttt ttcgggtggt aaagattccg gattaatgct gcatctgacc
      241 gccgaaattg cccgacaaat gggcaaaaaa atctgcgttt tgtttatcga ctgggaagcg
      301 cagttctcat gcactattaa ctatgttcag tcattacgtg agttttacgc cgatgtcatc
      361 gaagagtttt actgggttgc gctcccgctt acgacgcaaa attccctttc tcaataccaa
      421 cccgaatggc agtgctggga acctgatgtc gaatgggtgc gtcagccccc gcaagatgcg
      481 ataaccgatc ctgacttttt ctccttttac cagcctggca tgaccttcga acaatttgtg
      541 cgtgagtttg ctgaatggtt ttcacaaaaa cgtccggcgg cgatgatgat cggcatccgt
      601 gcagatgagt ccttcaaccg ttttgtcgcc atcgccagtt taaataaaca acgttttgcc
      661 gatgataagc cctggactac tgccgcacca ggcggtcata gctggtacat ttaccccatc
      721 tacgactgga aagtggctga tatctggacc tggtatgcta accatcaaca gctctgtaac
      781 ccactgtata atataatgta tcaggcaggc gtccctctgc gtcatatgcg aatttgcgaa
      841 ccttttggcc cggagcaacg gcaaggatta tggctctatc acgttatcga accggatcgc
      901 tgggctgcta tgtgcgcacg agtcagcggg gtaaaaagtg gcggcattta cgccggacat
      961 gacaatcatt tttatggtca tcggaaaatc ctcaaaccag agcatttaga ctggcaagag
     1021 tatgcattat tgctgcttga tagcatgccg gagaaaaccg ctgagcatta ccgcaacaaa
     1081 attgccattt acttgcattg gtatcagaaa aaaggtgtcg aggtgccaca gacccagcac
     1141 ggggacattg gcgcgaaaga tatcccctcc tggcggcgaa tatgcaaagt tttactcaat
     1201 aacgattact ggtgccgggc attatcattt agtcctacga aagcgaagaa ctatcagcgt
     1261 tataacgaac ggataaaagg aaaacgtcag gaatggggga tactatgcaa caacgattaa
     1321 cgcaggattt aacccaattt ctcgccagct tgccggaaga tgatcgcatc aaagcgatta
     1381 atgaaatccg catggcgatc catcaggtta gcccctttcg tgaagagcca gtggattgcg
     1441 ttctgtgggt taaaaacagc caacttatgc ctaatgatta caacccaaac aacgtggcac
     1501 cacccgaaaa aaaactgctg aaaaaatcca ttgaaataga tggttttacc caacccattg
     1561 tggtcacgca tacggataaa aatgccctgg aaatcgtgga tggttttcac cgccatgaaa
     1621 ttggtaaagg ctcatcatcg ttaaaattac gcttaaaagg ttatttgccg gtgacctgtc
     1681 tggaaggtac gcgtaatcaa cgcatcgccg cgactattcg tcataatcgc gctcgtggac
     1741 gtcaccaaat cactgccatg tcagaaatcg tccgagaact cagccagttg ggatgggacg
     1801 ataataaaat tggcaaagag ctgggaatgg acagcgacga agtgttgcgc ctgaagcaaa
     1861 ttaacggcct gcaagagttg tttgccgacc gtcaattttc ccgcgcctgg acggttaaat
     1921 aactacagct ggcgcaggcg ttctgctgct gccagcaacg tcgattcctt cttggcaaaa
     1981 cagagacgaa tcagtttatg tgggaaggga tcggcgcaaa acaccgacag cggaatcgcc
     2041 gctacgccgt gctcccgcgt cagccactgg caaaactcaa catcatccag cgtagatacg
     2101 gcgctgtaat ccaccagcaa aaagtaagta ccttcgcacg ctaagatctc cagccgactc
     2161 tcacttaagg cattcaccag aatatcgcgc ttctggcgat aaaagtccgg taacgcaaga
     2221 tagtgctcag gttctgcatg tagcatatcg gcaatcgcca gttgcgccgg ggtattcacc
     2281 gaaaaggtca gatactgatg cactttgcgg atctcggcgc tgatgggcgc aggtgcaacg
     2341 caatagccca ctttccagcc ggtcatatga taggtcttgc caaacgatga caccgccacc
     2401 gctcgctcac gcaattgcgg atgcgccagc acgctggcat ggccctgttg tgaaaaattg
     2461 atgtgctcgt agacttcatc gctaatgaca aaaatctcgt gcccggcgat cgcctgccac
     2521 agagcggcga aatcagtctg ctgccagaca gttgcactgg ggttatgcgg ggtgttgagg
     2581 atcaccagtc gggtgcgctc gcttaacaat gcggcaaatt cctgccagtc aacgcgaaaa
     2641 tgcggcggtt gcagtgcaat acgtttcact attccccccg aaagtgcgat ggcgggggcg
     2701 taactgtcat agctgggatc aaaacaaatc acttcgtcgc cattgcgcac caatgcggtt
     2761 atcgccgcgt ataacgcctc cgtcgcccct gccgtcacgg tgatattgct gtcgacatcc
     2821 ggttgatagc catacagacg ttccgttttc tgagcaatcg cctcacgcaa ggcctgcacg
     2881 ccggtcatgg gtgcgtattg gtttgccccc tgggcaacgt ggtacgccag ccgctcctgt
     2941 aaatagcgcg gaccatcaaa atcaggaaag ccttgcgaaa ggttaatcgc ctggtgttgc
     3001 tgcgccagcg cgctcatccg ggtgaaaata gtggtgccaa gttgtggaag tttgctttgt
     3061 ggaatcagag ggttatttgt catttttatt gtacctgacg aagttgcgtg ttgaaggcac
     3121 tataacacga tgctagtatt tggcaatcaa gacgtttaga tgtctaaata aaacaataag
     3181 gacaacacaa catgcctcac aatcctatcc gcgtggtcgt cggcccggct aactactttt
     3241 cacatccagg aagtttcaat cacctgcacg attttttcac tgatgaacaa ctttctcgcg
     3301 cggtgtggat ctacggcgaa cgcgccattg ctgcggcgca aacaaaactt ccgccagcgt
     3361 ttgaactgcc aggggcaaag catattttgt ttcgtggtca ttgcagcgaa agcgatgtac
     3421 aacaactggc ggctgagtcc ggtgacgatc gcagcgtggt gattggcgtc ggcggcggcg
     3481 cactgctcga caccgcgaaa gccctcgccc gccgtctcgg tctgccgttt gttgccgttc
     3541 cgacgatcgc cgccacctgc gccgcctgga caccgctctc cgtctggtat aacgatgccg
     3601 gacaggcgct gcattatgag attttcgacg acgccaattt tatggtgctg ctggagccag
     3661 agattatcct caatgcgccg caagaatatc tactggcggg gatcggtgac acgctggcga
     3721 aatggtatga agcggtggtg ctggccccgc aaccagaaac gttgccgcta accgtgcggc
     3781 tagggatcaa taatgcgcag gccattcgcg acgtcttgtt aaacagtagc gaacaggcgc
     3841 ttgccgatca gcaaaatcag caattaacgc aatcattttg cgatgtggtg gatgccatta
     3901 ttgctggtgg tgggatggtc ggtggtctgg gcgatcgttt tacgcgtgta gcggcggccc
     3961 atgccttgca taacggtctg accgttttgc cacaaaccga gaagtttctc cacggcacca
     4021 aagtcgccta cggaattctg gtgcaaagcg ccttgctggg tcaggatgat gtgctggcgc
     4081 aattaaccgg ggcgtatcag cgttttcatc tgccgactac gctggcggag ctggaagtgg
     4141 atatcaataa tcaggtggag atcgacaaaa tgattgccca caccctacgt ccggtggaat
     4201 ccattcatta cctgccagtc acgctgacac cagatacctt gcgtgcagcg ttcgaaaaag
     4261 tggaatcgtt taaagcctga cgtacaaatt tagcagcagc gcgcgccgcc ttttccaccg
     4321 tagcgcgcgt cctgccgctc acggaaaaac tcctcgtagg tcatcggcgt ttgatcggga
     4381 tggttaaccc gcatatgttc gacatagttg tcgtaatcag gcataccaat catcagtttc
     4441 gccgcctgac ctaaatattt tccggctttt gccagtgaat caaacatcta aacctccggt
     4501 aatacaaatg ccctcctgaa ttaagagggc ttaattgcta tcaacacgct cgggctgaga
     4561 gtaaggggaa cgctcagcac atcccccctc actctgactt tagtgcgcgc cttttgcctg
     4621 cgccacgatc tcctcgacat tttccggcat cggctcatat ggcgtttctt tcgccgttgg
     4681 cttcggctct ttcaatgccg ccagtgccgt cttaatcgag aacagcgcca gaaccaccac
     4741 aaccaccata aagaagatgg ttaatccggc atccagacgg ttgttgaaca ccagttgcgc
     4801 cagttgtgac tcagtatact gcgacggaat attgccgctg tcgatcattg cctggaactt
     4861 gttagcaatc gccaggaatc cgactttcgc atccgggcta aacgctttct gccagcctgc
     4921 ggtcagggta caaatcagca gccaggccgt tggtaccagc gccacccagg cgtaacgttg
     4981 acgtttcatt ttgaacagca ccacagcaca gagcatcagc gccatccctg ccagcatctg
     5041 gttggcaata ccaaacagcg gccacaaggt gttaataccg cccagcggat cgaccacgcc
     5101 ctgatgcagg aagtagcccc acgccagcac gcacagcgcc gttgccagca ggttagcagg
     5161 cagtgaatcg gtccgtttca ggcccggaga caccacgccc agcagatcct gcaacataaa
     5221 gcgcgcagca cgcgtacccg catccaccgc cgtaagaata aacagtgctt caaacagaat
     5281 ggcgaagtga taccagaacg ccacatccat catgccgccc agcgcaccgt gcagaatgta
     5341 ggccatcccc accgccaggg tcggcgcacc gcccgcacgg gaaatgatcg actgttcacc
     5401 cacttcgctg gcaatctggt ttagcgtatc cggggtaatc gcaaagcccc agctactcac
     5461 cacctgcgcg gcagaagcaa ccacatccgc cgtccctgcc ggagccagca cagccatcgg
     5521 gctgttcatg gcgaagtaca cacccggatc gatgatacag gcggaaacca gcgccataat
     5581 cgccacgaag gattccatta acatcccacc gtaaccgata aagcacgcct gcccttcgtt
     5641 cgccagcatc ttcggtgtag tgccggaaga gatcagtgca tggaagccag acaccgcgcc
     5701 acaggcgatg gtgataaaca ggaacgggaa caggttaccg gtccataccg ggccagtgcc
     5761 atcgacaaat ttagttagcg caggcatggt cagcgtcggg cgcataatca aaatgcccac
     5821 cgccagaccg acgatagtcc cgattttcag gaaggttgag aggtagtcac gcggagccag
     5881 cagcaaccac accggcagca ccgccgccac aaaaccgtaa cccaccagca tccaggtcag
     5941 ctgcacgccg gtaaagtcaa agtacggtgc ccaggtcgga ctttctgcca cccagccgcc
     6001 agagataatg gcgaaaatca ggaacaccag gccgatgacc gacacttcgc caatacgccc
     6061 cggacgcaga tagcgcaggt agatccccat aaacagcgcc agcggaatgg tgaacgcaac
     6121 ggtatacgtt ccccacgggc tatgagtcag ggctttaacc acgatcatcg ccagcacggc
     6181 aaggataatg accatgatca taaagcaggc caccagcgct atcaccccgg cggttggccc
     6241 catctcttct ttgaccagct cacccagcga gcgaccgtca cggcgcgtag aaacaaacag
     6301 caccatgaaa tcctgcaccg caccggcgag aaccaccccg gcgagcagcc agatcatccc
     6361 tggcaggtaa cccatttgcg ccgccagtac cggcccaacc aacggacctg ctccggcaat
     6421 ggccgcaaaa tggtgaccga acagcacttt tttgtccgtc ggcacatagt ccagcccgtc
     6481 gttatgacgc accgctggcg tcatacgcgt cgggtcaacc gccagcacat ttttggcgat
     6541 atacagccca taaaaacggt aagcgatcag atagatacag accgacgcca ccacaatcca
     6601 cagcgcgttg atctgttccc cacgatttaa agcaatgtat cccagagcaa atgctcccat
     6661 tacagagagc actgtccaga cgaggtattt ccctgatttg ttcatagttg ttatccgtgt
     6721 gcgtgtccat agagatgtta cattttgttt ctataacatc tttgtacaat ttaacaacac
     6781 agcaaccatg taaatcggag ttgaaaccaa tatttaactt aacaatgtta actcgatcac
     6841 tccgttaacc gaattttcct gcaatctcat tttattctgt cgggttcatc ccaaaaccgc
     6901 tgtgcccagc cgacaggtgc agcaacgccg cccctgttca tcgaaaacga cgatttccca
     6961 gctctgattt tgtcgtccaa gatgcagcgg ctggcagaca ccgcgcacct ttccctcaga
     7021 caccgggcga tgatgtgtgg cgttaagttc cgtgcccacc acacactgtc cgtcgcgggt
     7081 catcataaat ccggccatcg atcccagcgt ttccgccagc gccgccgacg cgccgccatg
     7141 cagcaggcca aacggctgat gagtacgggt atcaaccggc atttcggctt cgagtacatc
     7201 atcgcccaga cgggtataca caattcccag atgcgccacc attgtgttat cgctggtggc
     7261 gttcagttcg tcgagcgtta aatggcgttt ccagatcatg cttatgcccc cagcgtcgag
     7321 ccgccatcta ccacaatatc ctgtagggtg atatggctgg cgaggtcaga ggcgaggaac
     7381 aaaatcgtgt tggcgatctc ttgtggacgg gcgattttcc ccagcggaat gccgagttta
     7441 aactgctcgc caaagccgcg aatacgctgc tcttcggcgt catcactcac ccacagagtg
     7501 cgttgcatat cggtatcggt ggaacccggc gaaaccacat tacagcgcac cccgctaccc
     7561 gccagttcca gcccgacgct caacgccagg cttttcagcg ccgctttcga cgcgccataa
     7621 gcactcatac caatgcgcgg cgtgtgtgcg gcgtcggacg caacagtaac aatcgccccg
     7681 ccccgctgac ggcgaaactg gttcatggtc tgctgaaaca ggttaaatgc accgccgacg
     7741 ttgaccgcaa aagtctgctg ccagtcctct ttactgagct gatcggtcgc gcccatgcgc
     7801 aaaattcccg ccgcattgac cagcacgtcc agtcgctccg tttcagctaa cagtcgctga
     7861 catacctgcg ccacctgcgc agcatcggca acatccatta cttcggtcgc aaaaggatat
     7921 tgctcctgag taaacgcttg atcaaaacct gtaactttcg cccccgcctc aacaaatgcc
     7981 agcgccgtgg catagccgat acctttcccc gcgccagtta cccagacatt tttaccgctg
     8041 aaatccatta tttcacctcg cgggagagta gcttccacca ggcgtcgatg gtcgggtttt
     8101 tcgccagcat gacaaagtcg atatcgccat gcactttgcg ccagcgcgcc gccagcgcca
     8161 tcatgcgcac cgaatccaga ccgtagtcga tcaggttgtc gtcatcgaag ggttcatcgg
     8221 actcgtccag caacggcagg atcacctcac gcagctccgc tttgctggcg gggataggtg
     8281 ctggcagcaa ttcttcggtc atcaccaccc gaccagaacg tccggccaca tatttcagcg
     8341 acatcaaatg ctcgtcacgg ctgaaatcgg ccagcgcatc cgccaccata aacggtttaa
     8401 tatcgcgcat aaatgcgtcc gttgcggtgg tcatacagcc aatgtgggca tataccccgg
     8461 taataatcag ctggttacgt ccactctctt tgagcatttg ctccagcgga gaacgatgaa
     8521 atgcgctgta gcgccatttc accagcaccg tgtcgtcggc atctggcgtc aggcgatcca
     8581 ccaccttttg ctgttccggc gaacgggtca ggccaggccc ccacatatca ttcaacagcg
     8641 cccgatcttc atcgctctgc tctttcggct gggcggtgta ataaaccggg atattgtgct
     8701 gtttgcagta gtcgcgtagt gccgcaatat tcgcgatcac ctgctccatc atcgggcagt
     8761 tctcacccca gaagctgaca aaatagtcct gcatatcatg gattaacaac gcggcacgtt
     8821 gcggttcaaa ggcccagtcg actttattct gcggaatatc gtgaggctcc ggcagtgcgt
     8881 aagcctgtaa ttttggaata gccatcgtgt tctctccttc aggctgatgt gcgtgacgcc
     8941 agccactgac gtaattgttt tttatcgact ttcccgaccg ccgtcagcgg aagtgaatct
     9001 acacactcca cgcgatccgg taatttaaat tcggcaatac cctgttcacg caggaaacga
     9061 cgcacctgca ccgcgcgcag cggctctttt accaccagat aagcacagct tttttcgccc
     9121 atcagctcat cttccatgct caccagtgcg gcgtagatca ccgccggatg gcgcagcagc
     9181 aggttttcga tctcttcggc agcgatcttc tcgccgccac ggttgatctg atctttctcg
     9241 cgcccctgca cagtgatgta accctctgga tcaatagaga tcagatcgcc ggaacagtaa
     9301 aaaccgttgg catcaaaggc gctggcattg tgctgcgggc ttttgtaata gccacggaag
     9361 gtgtacggcc cgcgcgtcat caggcgtcca acttcccctt gcggcagtgg atttccttcg
     9421 gcatcggcaa cccatacttc atcgtccggg cacattgggt aaccctgggt atggataatt
     9481 ttctccgcac tatcatcaag acgggtgtag ttcaccagcc cttccgccat gccaaacacc
     9541 tgctgcaact gacagccaat ctcagcggga atacgcgccg caagggtggc agaaagacgg
     9601 gcgccgccga cctgtaacag tttcagcgag gcaagctgcg cccggctttc gccttcagcc
     9661 agtgcctgca accacaggct gactgctggc ggcaccagcg cggtgacgtt cacctgatgt
     9721 ttttcaatca atgggaagca aagcgtggcg ctggggtcgg cagccagaac gacagtgccg
     9781 ctagcgagaa atacccccaa cgatcccggt gaactcatgg cgtagttatg agccgccggg
     9841 atcgcgcaca ggtagcgcgt ctgttgtgtg aactgacaaa tctcgacgct acgacgcacg
     9901 ctgtagtagt agtcgttatg agtgcgcggg atcagtttcg gagtgccggt ggtgccgccg
     9961 gaaagctgga aataggccac ttcatcagca ggagatggcg tggcagtaaa atcctcagcc
    10021 ggatggttaa tcgcatcctg caagttatgc tcaccgctgt cgttgagcag ctgcaccacg
    10081 cgaatcgaag aatgctctgc aacaaatgtg ttgaggaaat catccccgct aaatagcgca
    10141 tgttggcgat cggcaatcag taacgccggt tcaatctggc tggcataggc gttcagttca
    10201 ctacgctgat ggctgaacag cgccagcacc ggcgcaacgc ccagtttcag cagcgcgaaa
    10261 aaggtaatgt aaagttcagc gacgttaccc agttgtacca gcgcggtttc accaggttta
    10321 atgccctgac ggcgcaaact acaggcgagg ttatccgccg cctgattcag ctcccggtaa
    10381 ctcaactgcc gctcgccgtc gatgaccgcg atgctgtcgc ttgcggcatg acgggtcaga
    10441 atgtcggtca gcggcaaatc ctgccagtag cctttttccc gatagcgacg ggcaaactct
    10501 tccggccagc gggtgaatgg aatgctcatc ctcgctcctt aatgcaatcc aaaaacgttc
    10561 aacatggtag aaagtttgac gcctgtttcg cgccactcac ccaacggtga ggacgcaggc
    10621 acaatccccg ctccggcaaa cagacgcacc tgattttccc gcagcttcgc gcagcggatg
    10681 gtcaccaccc attcaccgtt accttcgctg tcacaccaac ccacaatgcc gccaaacagt
    10741 tcgcggtcga acggctccag ttcagcaata acctgagtcg cggcctgatg cgggaaaccg
    10801 ctcagcgcgg gggtcggatg cagcagacaa gccaaagtca gtgcgttttc ttgcgaattc
    10861 gctttacctt caaagggagt tgcgagatgc cacagcgtcg gcgtggtaat caattgtgga
    10921 gaggaggtaa cgtgtaactc actactgcgt ttgcgcagta cctctttcat cgcctgagtc
    10981 accagttcat gttcatggcg atctttttct gacgccagca gacgattacc cgcttcgcga
    11041 tcgagcactt catccggctg acgacgcgcg gaaccggcta acggaatgga gctaaaacgc
    11101 tcgccgtctt tacgtagcag cagttccggg ctggccccca gcagaacgcc accatcagcc
    11161 aacggaacat ggaagttgta actaaccggg ttttgcgcaa tcaaccgttc cagcaatacg
    11221 ccactatcaa tggcggcgtc agtagtgata tcaatcaacc gtgacaacac cactttgtcg
    11281 acctgcggcg tggcggtaag tgcggcagca cgggcaacca tctgttcaaa cgtggtttgt
    11341 tccggaatgg cctgacgttc caccacgttc agcgactggc tgcgggtgaa acggcgtgcg
    11401 gaagcttgtt tttcctgacg ggagaacgac tgccaggatt caggtatata cagcgacgaa
    11461 ggctgacgtg gatcgaaggg aatcgccccg accatcaccg gatttttgat gccctgcgct
    11521 ttggcatcgg caaacaacgc ggcgagtttt tgctggaagg gactgtcggg cgaatccccg
    11581 ttcacagccg gttcatcgaa gcgggcgaaa catcctgacg tcgtaaaact gcggtacggc
    11641 gacataaaga aaaagcgatt gggcgcaagt gttgccatgg tctgctgtac ttcctcagcc
    11701 agtgacgtat ccatatcatc ctccacaaaa tgataaaggc tttaataatg attatcattt
    11761 atattttcgg tcgctaacct aaaagcaaac gcgcactatg tcaactcttg aggtaacgca
    11821 atctgcaact cggggttgca tctggttgtg gttacaatga aaatgagaag cattaacttc
    11881 attaataaca ggaagctgac ttgtgagact cgccccgctc taccgcaacg cccttctatt
    11941 aacaggactt ttgctttcag gaatagccgc agttcaggcc gccgactggc cgcgtcagat
    12001 tactgacagc cgtggcactc ataccctgga aagccagccg cagcgtattg tttccaccag
    12061 cgtcaccctg accggctcac tgctggcgat tgatgctccg gtgatcgcca gcggcgcgac
    12121 cacgccaaat aaccgcgtag cggatgacca gggcttttta cgccagtgga gcaaggtggc
    12181 gaaagaacgc aaactgcaac ggctctatat cggcgaaccg agtgcagaag ccgttgccgc
    12241 gcaaatgccg gatctgattt taattagcgc aaccggcggc gattcggcgc tggcgctgta
    12301 cgatcagctt tctactatcg ccccaacatt aatcatcaat tacgacgaca aaagctggca
    12361 gtcgctgtta acgcagcttg gcgaaattac cgggcatgag aaacaagcgg cagagcggat
    12421 tgcacagttt gataagcaac tggcggcggc gaaagagcaa atcaaattac cgccgcagcc
    12481 ggtcactgcc atcgtctata ccgccgctgc acacagtgcc aatctctgga cgccggaatc
    12541 agcacaaggg cagatgctgg aacaactcgg ctttacgctg gcgaagttgc ccgcaggctt
    12601 aaacgccagc caaagccagg gcaaacgcca tgacatcatt cagcttggtg gagaaaatct
    12661 ggctgcaggg ttaaatggcg agtcactatt cctgttcgcc ggtgatcaga aagacgccga
    12721 tgctatttat gctaatccgc tgctcgcgca cctgcctgca gtacaaaaca agcaggttta
    12781 tgcgctggga accgagacgt tccgcctgga ttactacagc gccatgcaag tgctggatag
    12841 acttaaggcg ctgttttaag cattaactgt cggacgctgt cacctgcggc ggcgtctggc
    12901 gaaaacgtcg caactccact agtaccagca gcaacaacac gccgataatc aacaaaccaa
    12961 aaccgctcgc acttgcggaa gcaaccggtg tcatcatcgc tcccaaaccg cccagcagcg
    13021 ccgcgcctat ggcatcgccc gtcacgtttt gcgccgtcca caaaccgtta atccgcccta
    13081 acatcgcttc cggggtttgc gtttgcagca ttgtatattg cagcaacgaa ctaaccgcac
    13141 tcagccagcc gaacagtgcc agacaaacca cgcctaaaat ccacatcggc atcaggccaa
    13201 acagaccaat ggcgaggaac gcccccaatg tggagagcaa catcaacaga cctggccgta
    13261 cgctatgcgc cagttttcca ctggttaacg cgccaatagc cgcaccgagc gggattgccg
    13321 catagagaaa gccaatttgc gccgccgaca tctgccagtt gtcagccagc gccggataca
    13381 ggacccgcac cgcgctcgcc atcgtcaata acccccccag caacgcaatc cctcccacca
    13441 gcgggctggc gagtagaaaa cgaaatcctg ccagtaatga tttcaacgga tgctcacgcg
    13501 gctgcggcgg tggtggcaac gccggaaggc ttaacaacgg tagcaaggta ataaacgtgc
    13561 ccgccgccgc cagcccgtag ttccaggcta cgcctccggt cgccagcagt aaaccgccaa
    13621 tcatgggcga aatcaccgat cccagacgca cggtcaacat ggtgatcgcc ccggcctgca
    13681 ttaggttttc acgccctacc agtgcaggtg tcgccgccag tagcgccgta acgccaagtg
    13741 atgcgaaaaa accatcccat aaaccaagta aatagattgc cagcaaagac ggctccggca
    13801 gcagcgcatt caggcacagt ccaatgaagc caatgccaca ggtgccacgc gccagcaaaa
    13861 tcactttttt acgctcatag cgatccgcca gcacaccgcc gaccatcagg ccaacaaaca
    13921 tcgcgccgcc ggtcagcgtc accgaaagcc ccacctgcca ggtagaatgc gtcatcatct
    13981 ggatctgcac cggcaccgcg acgccgagca aacccagaga aacaattgag ataaaacgag
    14041 cgaggaatac tgcgcgaaac gccgggtgcg ttttcaacag gctgaggtta agcagccagg
    14101 attgtttatt cattacaatg ccttgccatc aaatatttta aatacctatt cttaggctgc
    14161 acatgctaac atatccaaat aagatcgata acgataatta atttcattat catggaagtt
    14221 cgtatgtctg gttctgttgc cgtgacacgc gccattgccg tgcccggatt gctgttatta
    14281 ctgattatcg cgacggcatt aagcctactc attggggcaa aatcactccc cgcttccgta
    14341 gtgctggagg cgctctccgg cacctgccag agcgccgact gcaccatcgt gctcgacgca
    14401 cgactgccgc gcacccttgc cggtttactg gcaggcggtg cgcttggcct tgccggggcg
    14461 ttaatgcaaa ccctcacccg aaacccgctt gccgatcctg gtttgcttgg cgtgaacgcc
    14521 ggtgccagtt ttgccattgt gctgggcgcg gcgctgtttg gttactcttc cgcgcaggaa
    14581 caactggcga tggccttcgc cggggcgctg gtggcctcgt tgattgttgc ctttaccggc
    14641 agccagggcg gcgggcagtt aagtccggtg cgtttaaccc tggcaggcgt ggcgctggcg
    14701 gcggtgctgg aagggctgac cagcggcatc gccctgctta atcccgacgt ctacgatcaa
    14761 ctgcgtttct ggcaagccgg ttcgctggat attcgcaatc tacatacctt aaaagtggtg
    14821 ctgatctcgg tgcttaccgc tggagcaact gcgctattac tgagccgcgc gctgaacagt
    14881 ttaagccttg gcagtgacac cgcgacggca ctgggcagtc gcgtagcgcg cacacagttg
    14941 attggtctgc tggcaattac cgtgctttgt ggtagtgcga cggcggtagt tggcccgatt
    15001 gcctttattg gcctgatgat gccgcacatg gcgcgctggc tggtaggtgc cgatcatcgc
    15061 tggtcgctgc ccgtcacgct actcgctacc cctgccctgc tgctgtttgc cgatatcatt
    15121 gggcgggtga ttgttcccgg cgaactgcgc gtttctgtag tcagtgcctt tattggtgca
    15181 ccagtgctga tcttcctcgt acgacgtaaa acgcgaggtg gcgcatgatt tacgtctctc
    15241 gccgattaat tatcacctgt ctgctgttgc taatagcttg tgtgatggca ggcgtgtggg
    15301 gattacgtag cggtgccgtc acgctggaaa cttcgcaggt attcgccgcg ttgatgggcg
    15361 acacgccgcg cagtatgacg atggtggtca ccgaatggcg tttaccacgc gtgctgatgg
    15421 cgctgttgat tggcgcggcg ctgggcgtca gcggcgcgat ttttcagtcg ctgatgcgta
    15481 acccgctcgg cagccctgac gtaatgggct ttaacaccgg ggcatggagc ggcgtactgg
    15541 tggcgatggt gctgtttggt caggatctga cggctatcgc gctggcagca atggcggggg
    15601 gtattgtcac ctcgctgctg gtctggctgc tcgcctggcg taacggtatc gacacctttc
    15661 ggttgattat tatcggcatc ggcgttcgcg ccatgctggt ggcctttaat acctggctgt
    15721 tgctgaaagc gtctttagaa acggcgctaa cagcgggttt gtggaatgcc ggatcgctca
    15781 acggcctgac gtgggcaaaa acctcgcctt ccgcccccat cattatatta atgttaattg
    15841 ccgccgcctt gctggtgcgg cgaatgcgct tgctggaaat gggcgatgat accgcatgtg
    15901 cgctgggcgt cagcgtcgaa cgttcgcgac tgttaatgat gctggtcgcc gtagtgctta
    15961 ccgcagctgc cactgccctt gccggaccga tttcctttat tgctttagtc gcgccacaca
    16021 ttgcccgccg catcagtggc accgctcgct gggggctaac ccaggcggcg ctgtgcggag
    16081 cgctgttact gctggcggcc gatctctgcg cccaacaact gtttatgccg tatcaacttc
    16141 cggttggcgt cgttaccgtc agcctcggcg gtatttacct tatcgtcttg ttaattcagg
    16201 agtctcgcaa aaaatgaccg aatcagtagc ccgtttgcgc ggcgaacagt taaccctcgg
    16261 atatggcaaa tataccgttg cggaaaatct gactgtagaa attcctgatg gtcacttcac
    16321 ggcaattatc gggccaaatg gctgcggtaa atccacgtta ctgcgtacct taagccgcct
    16381 gatgacgcct gctcacgggc atgtctggct ggatggcgag cacattcaac attacgccag
    16441 taaagaggtc gctcgccgga ttggtctgct ggcgcaaaac gctaccacgc cgggagatat
    16501 caccgtacag gagctggtgg cgcgtgggcg ttacccgcat caaccgctgt ttacccgctg
    16561 gcgcaaagaa gatgaagacg cggtaacaaa agcgatgcag gccacgggaa taactcatct
    16621 ggcggatcaa agcgtggaca ccctttctgg cggacaacgc cagcgagcgt ggatcgcgat
    16681 ggtgctggcc caggaaacgg cgattatgct gctcgacgaa cccactacct ggctggatat
    16741 cagtcatcag attgatctac tggaactgtt aagcgaactg aaccgcgaga aaggctatac
    16801 cctggcggcg gtgctgcacg atcttaatca ggcctgtcgt tacgccagcc atttgattgc
    16861 attgcgggaa gggaaaattg ttgctcaggg agcaccgaag gagattgtca ctgctgaact
    16921 gatcgagcgc atttatggtc tgcgctgcat gattattgac gatccggttg ccggaacgcc
    16981 gctcgtggtg ccgctcggac gaacggcacc ttcaaccgca aagatttaaa ctaagtggtc
    17041 tgccatcatg gcatcctgtt ttctggatgc catcgcatga cgcaacaaca caccaccaca
    17101 agccaccatc ccgccgacca acgcagaaag gatcacgata atcgccttac cctgaccgtc
    17161 tttttttacg ggtaatgacg gacgcaactg atatttaaac ggtgtaaaat tcacatcgtt
    17221 gatatttgtt tttgttaatt gctcgactag atactgacga ttacgtaatt caccgttcaa
    17281 ttccgcaacg tcagtgactg ctttttcaat ttccagtttg cgttcaatac cgtctgcacc
    17341 gagagaaata gagaaatcgg ggtcatcttt aacggcctga ccattactgt atacgggctt
    17401 tttaattcct gccgcgttgg caatgtcaag tgaataattg aggcgctgaa tgtttgcatc
    17461 aagttgattt ttcgttttaa tgcgatcctg agccagtttt tctttttcaa actgggtttt
    17521 gatctccagt ttattacgga cgttttctat cgactctttc accaccaacg cagagatata
    17581 atcgatatac ccggccaaca ctttctgtgc ctcttcactg gtgggtgcgg tgaaacttag
    17641 cgtccaggaa gtatacaatg cagactcatc ttttttctta ctggcattgt catcaaccgc
    17701 tttcattttt tcgctcaatg caacaatggc gcgatgcaga tccagtgggt cgattttcgc
    17761 ttcttttaat tgatccatca cataaggtga tgaacgcagg tactctcaag caagctaaca
    17821 gactgaaatt tcttgataaa caggttaaat gcttctgtac gatcaatttt gatatccaga
    17881 tccagcacac gaagcttagt gaatgttttc tccagctctt gccactgaac aggttctgga
    17941 ggcgtgacaa ccgccgcgct agtccatttt tgcggcagga tgaaagagat acgcaaaaac
    18001 gaccgccatg accgtttttt ttgcccgcca taaaacctcg attagattaa gtaaatcaat
    18061 ttcattatta ctgggcgacg ccagaggata ctcgggaaaa tgagcttcac ttccctgttt
    18121 aatattcagt gatgacatgt agtgaaacct gaggttttag tccgtaaagc acaacgatgt
    18181 acatagcatt ctaacaggaa ctgagattta tccgaaagca ctctttcact attattattc
    18241 tcaaaatgaa taacaaactt agcaacccca gcaatgacgc tgctctcaga aaaataaata
    18301 aattttaaga ataaaaaaac cataatcatt tagtcggatt atggttttac atgaggttat
    18361 ggcaagttct ttactaacgt acggttagtt attaatttgt gtatttatta gtggtcctat
    18421 ttctttaaaa tactccgggg aaataatatc cacatgcgca caatcctgac gatagatatc
    18481 cagctcagca atccacggtg cccaggcctg ttcaggagtc ataccttcct gaagcgtgcg
    18541 ttcggcaaca aatagcgtcg ctttaccatc aaacggtacg ctatgagctg tcgtcagcag
    18601 gcgcacagca tcagcgtagt tgccttcaat ggtggtaaac aactccgttg aagtacttcc
    18661 ctgctgtgcc gccaggaagg cttcgcgctc gcggttaatc tccgccagca cttccgggtc
    18721 cagaccattg gcttcttttt cctgccagtt ttgcgtttct ggcggccagg tatccagcaa
    18781 gccaagaaat gccacctgtt cgccacgggc acgcagtcgc gccgcaatgc cctgcgccag
    18841 cgtaccgccc agcgaataac ccagcaggta atatgggccg tgcggttgtt gttcaagtaa
    18901 ggttgccaga tgcgcttcgc agacttcatc cagatttgct gacgtctgca tggggccatg
    18961 agggcgcggc gactgaatgc cgataatcga ccattgtgga tcgagataac gcgagagcac
    19021 gctgaactgc caggcaaaac cggatgccgg atggaaacaa aacagcgtcg gaccattacc
    19081 ttcacgcaac ggcagaatgg tttcgaatcc catgcgctgg gtgctgtctt cttcaccatc
    19141 aataatcgtt gccagtttgg cgacagttga cgcgaccatc acctgccccg gcgtcacctg
    19201 acgagcaaac tgtcgactta actgcgctgc cagtttcatt gccagtagcg aatgaccgcc
    19261 aagcgcaaag aaatcagcat cggcatcctg cacgtcacaa cccagcaacg acgagaatgc
    19321 cgcggcgata atcgtttcac tgcccgcttt cggcgcacgc ccaagcgctt gtgccttcag
    19381 ttcaggcaac ggtaaggctt tgcgatccag cttgccgttg gcgctaagtg gtaactgtgg
    19441 aagttgcagc agaaccaccg gtaccatatg tggtggcaat gtttcacgaa gctgcgcctg
    19501 taatgcgctg gtatccaacg gcagacctga ttgcgacacc agatagccca ccaactgacg
    19561 cgcatcacca ccagtggctg ccgcctggtt aatcacacag gcgtgggtaa cggcttgttc
    19621 gacatccggc agcgcctgca tcacgcgatc aatttcgccc agttcgatac gctgcccgcg
    19681 aatttttagc tgatcgtcac tgcgcccgag gtactccact gcgccgttat ccagccagcg
    19741 ggccacatcg ccggtacggt acatccgttc acctggggca aaaggatcgg caataaagcg
    19801 gctggcggtc agatcgggtc gtccaagata cccctgcgcc agttgaatac cggtgagata
    19861 gagatctccc gccacccccg gcggcaccgg atgcatcatc gcatcgagaa tgcgcaggcc
    19921 cgtattccac accggataac caatcggcac actgctgccg cgcacctctg ccagttcctc
    19981 gccaaaagcc ggataccaac tgacatctac cgccgcttcc gtcgggccat atagattatg
    20041 caacggcgcg ccggttaact gttgccattc gcggcataaa tcagccggta aggcttcacc
    20101 actacagaaa acctgtttca acgtcgcgca actctggcga gcggtttgcg gcgtcagcga
    20161 tgcaacaaat gccgccagca tcgacggcac aaagtgcgtg gtcgttacgc cataatcagc
    20221 aaaaaaatgc tgcatagcga gcgggtcgcg gtgcgcttcc ggttcagcca tcacaagttt
    20281 cgccccggca ataaacggcc agaaaaactc ccacaccgag acatcaaaac tgcacggcgt
    20341 tttttgggca acaacatctt cgcctgtaag cggatagtga ttttgcatcc ataacaggcg
    20401 gttaacgata gccgtctgcc cgaccattac ccctttcggt ctgccggtgg agccagaggt
    20461 aaagatgata taagccgtgt ggtgcggttg tgaaagctgc agcggcgcac tgccctgcgg
    20521 tgtcagcggg gcgttatagc aaaggctggt taaattgggg atatcgctaa aacgcggcag
    20581 ttgatcgtcg gtggtgatta acagcgacgg acgcgcatct tccagcatca ttttcaggcg
    20641 atcgtccgga taccccgtat ccagcggtag ccaggccgcg ccagcttcaa ctatggcatg
    20701 tagcgccagg gtcaaaaaga ccgagcgcgg tagcgccacc gccacgctgt cgcctggttt
    20761 aacgccgcgc tcacgcagca gattcgccag cgccaccacc tgttcgcgca tttcccgata
    20821 gctgaactgg taacgcgtat ctgccagcgc cggagcatcc ggtgtttttg ccgcttgttc
    20881 tgccaccagc gcgctcagcg tggtttctgg aatctcaacc tgagtggcgt tgatctgcgc
    20941 cagctgcgca tactcacctg gcagcataat atcgacatcg ccgcacaaca gcgacggatt
    21001 cgcagcgaac tgcgcaatca gcattttcag gcgttcagcg tgttgaatta acgttggctc
    21061 atcgtaacgc tgtttattgg cgaggatttc aatactcaaa tcaccgtgtt catccgggaa
    21121 cagggccagt tcaaggtcat taaccggacc ggttgccagg gtatgggttt gcgcctgaac
    21181 accaggaata tccagttggt aatcaaatac cttgatattg agtaccggac cgaacagcgg
    21241 ttcctctcct gccgctcgcc cactgtcacg gacaatttgt tcggcatcgt aacgttgatg
    21301 gcgacgcatt tttttcagtt gagccgccag tcgggttgcc agttctggta gcgtttcttg
    21361 cgccgcaatg tgaataccta atggcaaaac gttgagcacg ggtccggtag ccgtcagcgc
    21421 cgccgagccc agtcgacgca taaagataaa tccggcagcg tagtccatgc ggttgcacaa
    21481 tcgccccagc cacaaagctg ccaacgcaag ggctaaatcg gtacgctgca cacctgaaag
    21541 ttgcgtagcc agctggcgga attccccgtc ggtaaattcc agtttcaggc gcaaaatatc
    21601 tgccgaagcg ctgcgccccg gtaaaggtgc cggagaaagt gacgcgggcg gtggcagttg
    21661 acgacgctgt tccgcccaga atgccgcatc acgctgccag gcttcacttt cgcggtactg
    21721 ctgatactct tccactacat cagcgaaagg cgtaaacggc gaagcaggcg ttggttcgcc
    21781 acgcagccat gcgcagtaaa tattggcgat ctggcgtgta atggccggga aactgaagcc
    21841 atcgaccagc aaatggtgat aacgctgata ccagtaccag cggttatccg ccacctgtat
    21901 cagctgatga aagaccagtg gtttaccact atcgacgcgc agatcttgtt gcaaatccgc
    21961 ctgcattaat gcccgcgcag taccgtgcgg atcaatgttg gttcgcaggt cgataatttc
    22021 tggcagttcg aacgtctgcg catcatcgac ccactgccag acttcgccgt tatcttccgt
    22081 aaaacgcatc cgcaacgtat ctgcttgcgc tagtccggca accaccgcgc gggccagtag
    22141 tggcgcatca acctctccgg ttaactcaac gtaatgcgcc acgctccagg cggagggtaa
    22201 atccgacagt ttttctgcca tccagatgcc gggctgtgcg gcgaccaaag gtaaatgctg
    22261 gctcattgtg cctcctgcaa ctggatgaaa ttcgccggtg tcagagtacg ccagtgggct
    22321 tccagccact gctggcagga cgcctgtgac tgcggctgac acacaacgtc ccagcctgcc
    22381 ggtaaggcgc attgttgcgg ccacagactg aattgcccct gcgcattgcg caaaatgtaa
    22441 aacgctccct gtggatcatc gaagggatta ctgaatgcca tattcaactc ctgtcatgga
    22501 aaagtggttg ccagaggtcg attagcccct gcatcaagcc accgcgccaa caaagcgcat
    22561 catgtccgcc gtcaacctga cgccagaaaa tggattcttt tagggggtgt aattgcgcat
    22621 acagcgcctg attggcccgc atgatcatcg gctcgcgaac acccgcttcc agcacaatgc
    22681 gcagaccttc ggcgctaact tcaccagtat ttagctgttc aagtaacatg ccctcttggt
    22741 gcccgccccg atgcggccac cagtacgaac ctgactggct taatacacag ccaaagcgtt
    22801 caggccagtg cagtccggca tacagcgcgg aaagcccacc aaaactctgt ccggcgacca
    22861 cggtgcgatc ggcgcgatcg ctaaaagggg caatagcttt cacctggggt aataactctt
    22921 gctgtactgc cagccagaaa tccgcattac acggcagttc gtgggcgcgg tgcgtggtgt
    22981 cgatagcgtc gatcaacaca tatacggcgg gcggaagttg ccgacgatgg gtcagcgaag
    23041 tcagcgcagg ccagacgggc atactctgcg cccaaaattc gccatcaagc aaaactgcca
    23101 gcgggcgttc ttctgctgtt gcatcgccgg tggtaaaaat ccatacacgg cgtgaatttt
    23161 tcaaccgttc acttttccag ataatttctt tggcaggcgt ttctggcgct tgcggacaat
    23221 cccatcccgg ttgcagaggc gcttgcggca tttcgagtgc agaaacagcg tgccctcgcc
    23281 cgcctttcca gctttgtgga ttcagcggat cggctatcgc ctggggtaat agttttcgcc
    23341 agccttcgcg taattcgagg cgatcggggg atggtgcaga aaaaatgtca tcgcgttcag
    23401 taggaataaa gcagtagctg ccgcgccagt tggcattgag ttgtgtcgtc cactgccaaa
    23461 catcggttcc tgcaattcgc tgcatcgacc ggggctggct gttctgatgg tgatcggtca
    23521 cgccagtgat atagacccat acgcgcttta tcgtcgagta ttcttcagaa ccctggggat
    23581 cacgccacca gaaagtgacc tcaaacattt cgtcattcag acgttgccat tccgggccat
    23641 gtttcgactg ccaccagctc tcacttccta cttttaacgc cgtcacacca taaccccatg
    23701 tttactgtgc aattttttat tgattgcaga aatatattga taatattatt gataactatt
    23761 tgcatttgca atagcgtaat ggcgcgccgt gagaagcgcg gacattaatt aatcagctgc
    23821 actgcgtgtc tttcaggatc aaaggttttc gcggtagcgg gatgcgtcgt gttgatggcg
    23881 accatgcccg acagttgcaa ttcgtggcaa aaatgcagga ataaaacaat gaacaagaag
    23941 attcattccc tggccttgtt ggtcaatctg gggatttatg gggtagcaca ggcacaagag
    24001 ccgaccgata ctcctgtttc acatgacgat accattgtcg ttaccgccgc cgagcagaac
    24061 ttgcaggcgc ctggcgtttc gaccattacc gcagatgaaa tccgcaaaaa cccggttgcc
    24121 cgcgatgtat cggagatcat tcgtaccatg cctggcgtta acctgaccgg taactccacc
    24181 agtggtcagc gtggtaataa ccgccagatt gatattcgcg gcatgggtcc ggaaaacacg
    24241 ctgattttga ttgacggcaa gccggtaagc agccgtaact ctgtgcgtca gggctggcgt
    24301 ggcgagcgcg atacccgtgg tgatacctcc tgggtgccgc ctgaaatgat tgaacgtatt
    24361 gaagttctgc gtggtccggc agctgcgcgt tatggcaacg gcgcggcggg cggcgtggtt
    24421 aacatcatta ccaaaaaagg cagcggtgag tggcacggct cctgggatgc ttatttcaat
    24481 gcgccagaac ataaagagga aggtgccacc aaacgcacca acttcagcct gaccggtccg
    24541 ctgggcgacg aattcagctt ccgcttgtat ggcaacctcg acaaaaccca ggctgacgcg
    24601 tgggatatca accagggcca tcagtccgcg cgtgccggaa cgtatgccac gacgttacca
    24661 gccgggcgcg aaggggtgat caacaaagat attaatggcg tggtgcgctg ggacttcgcg
    24721 cctctgcagt cactcgaact ggaagcgggc tacagccgcc agggtaacct gtatgcgggt
    24781 gatacgcaga acaccaactc tgacgcttac actcgctcga aatatggcga tgaaaccaac
    24841 cgcctgtatc gccagaacta ctcgctgacc tggaacggtg gctgggataa cggcgtgacc
    24901 accagcaact gggtgcagta cgaacacacc cgtaactcgc gtattccgga aggtctggcg
    24961 ggcggtaccg aagggaaatt taacgaaaaa gcggcacagg atttcgtcga tatcgatctt
    25021 gatgacgtga tgctgcacag cgaagttaac ctgccgattg atttcctcgt aaaccaaacg
    25081 ctgacgctgg gtacagagtg gaatcagcaa cggatgaagg acttaagttc caacacccag
    25141 gcgctgaccg gaacgaatac tggcggtgct attgatggtg tgagtgccac cgaccgtagc
    25201 ccatattcaa aagcagaaat tttctcgctg tttgccgaaa acaatatgga gctgactgac
    25261 agtaccatcg taacgccggg gctgcgtttc gatcatcaca gtattgtcgg caataactgg
    25321 agcccggcgc tgaacatatc gcaaggttta ggcgatgact tcacgctgaa aatgggcatc
    25381 gcccgcgcct ataaagcgcc gagcctgtac cagactaacc caaactacat tctctacagt
    25441 aaaggtcagg gctgctatgc cagcgcgggc ggctgctatc tgcaaggtaa tgatgacctg
    25501 aaagcagaaa ccagcatcaa caaggagatt ggtctggagt tcaaacgcga cggctggctg
    25561 gcgggcgtga cctggttccg taacgattat cgcaataaga ttgaagcggg ctatgtggct
    25621 gtagggcaaa acgcagtcgg caccgatctc tatcagtggg ataacgtacc gaaagcggtg
    25681 gttgaaggtc tggaaggatc gttaaacgta ccggttagcg aaacggtgat gtggaccaat
    25741 aacatcactt atatgctgaa gagtgaaaac aaaaccacgg gcgaccgttt gtcgatcatc
    25801 ccggaatata cgttgaactc aacgctgagc tggcaggcac gggaagattt gtcgatgcaa
    25861 acgaccttca cctggtacgg caagcagcag ccgaagaagt acaactacaa aggtcagcca
    25921 gcggttggac cggaaaccaa agaaatcagt ccgtacagca ttgttggcct gagcgcgacc
    25981 tgggatgtga ccaagaatgt cagtctgacc ggcggcgtgg acaacctgtt cgacaaacgt
    26041 ttgtggcgtg cgggtaatgc ccagaccacg ggcgatctgg caggagccaa ctatatcgca
    26101 ggtgccgggg cgtataccta taacgagccg ggacgtacgt ggtatatgag cattaatact
    26161 cacttctgaa gctaacgtca gattgttgac aaagtgcgcg tagttcatgc cggatgcggc
    26221 gtgaacgcct tatccggcct acaaaaatct tgccaattca atatattgca ggaccacgta
    26281 ggcctgataa gcgtagcgca tcaggcagtt ttgcgtttgt catcattctc taatatggtt
    26341 gacatgaaaa ctacgcatac ctccctcccc tttgcaggac atacgctgca ttttgttgag
    26401 tttgatccgg cgagttttcg cgagcaggat ttactctggc tgccgcacta cgcacaactt
    26461 caacacgctg gacgtaaacg taaaacagag catttagccg ggcggatcgc tgcaatttat
    26521 gcgctgcggg aatatggcta taaatgtgtg cctgcaatcg gcgagctacg ccaacctgtg
    26581 tggcctgcgg gggtatacgg cagcattagt cactgtggga ctacggcatt agccgtggtt
    26641 tcccgtcaac cgattggcat tgatattgaa gagattttct ctgcacaaac cgcaagagaa
    26701 ttgacagaca acattattac accagcggaa cacaagcgac ttgcagactg cggtttagcc
    26761 tttcctctgg cgctgacact ggcattttcc gccaaagaga gcgcatttaa ggcaagtgaa
    26821 atccaaactg atgcagattt tctggactat cagataatta gccggaataa acaacaggtc
    26881 ataattcatc gtgagaatga gatgtttgct gtgcactggc agataaaaga aaaaataatc
    26941 ataacgctgt gccaacacga ttaattgaca acatctggta cgattcgccc gcagccatca
    27001 ctgaccgcgg gcgaaagtgt aaagcaggtg ccttaccacc ctgacctgac aaccggatat
    27061 gcggggattg ctccccgcac aacggctact tcttcggttc gtaagcgaga acagccttaa
    27121 actcaatatt acgttccttt acggtaaact cacacagcga gtctccgacc agaagcgtaa
    27181 atcccagcac cgttaaacac agcactatga ctgccacaag ggcatatttc gtcagcatgt
    27241 ttatattgcc tccatgcgaa gaagggacta ccatccaaat tgttcaggtt tgggtaggta
    27301 gccccagggt tgatagaaat atcgtcctgg ggcttcatcc ttccagacct cagagtaaac
    27361 ctgaaaccgg aaagcttcag gcacccgcag ttatcttacc cttcttcagc aaaatagctg
    27421 tttatttata cagtaagtcc tgtttcggac ttctcactcc tcatttgatc aactgaagct
    27481 gaacccgttt ttttctgcat gcctcaatcg tcaaaattct tcgccaccag ccagctctgc
    27541 cataacttgt cacattgcaa aagtagccaa attgcttcag tcaccatcag ttcaccatcg
    27601 ccaaaaaaaa gaaacattcc agaacttccg catctcaccc ggccgctccc acttctatac
    27661 tgaatagaaa acgccaatat aagagaaacc tatgccatta cccgattttc atgtttctga
    27721 accttttacc ctcggtattg aactggaaat gcaggtggtt aatccgccgg gctatgactt
    27781 aagccaggac tcatcaatgc tgattgacgc ggttaaaaat cagatcacgg ccggagaggt
    27841 aaagcacgat atcaccgaaa gtatgctgga gctggcgacg gatgtttgcc gtgatatcaa
    27901 ccaggctgcc gggcaatttt cagcgatgca gaaagtcgta ttgcaggcag cggcagacca
    27961 tcatctggaa atttgcggcg gtggcacgca tccgtttcag aaatggcagc gtcaggaggt
    28021 atgcgataac gaacgctatc aacgaacgct ggaaaacttt ggctatctca tccagcaggc
    28081 gaccgttttt ggtcagcatg tccatgttgg ctgcgccagt ggcgatgacg ccatttattt
    28141 gctgcacggc ttgtcacggt ttgtgccgca ctttatcgcc ctttccgccg cgtcgccata
    28201 tatgcaggga acggatacgc gttttgcctc ctcacgaccg aatatttttt ccgcctttcc
    28261 cgataatggc ccaatgccgt gggtcagtaa ctggcaacaa tttgaagccc tgtttcgctg
    28321 cctgagttac accacgatga tcgacagcat taaagatctg cactgggata ttcgccccag
    28381 cccccatttt ggtacggtgg aagttcgggt gatggatacc ccgttaaccc ttagccacgc
    28441 ggtaaatatg gcgggattaa ttcaggccac cgcccactgg ttactgacgg aacgcccgtt
    28501 taaacatcag gagaaagatt acctgatgta taaattcaac cgtttccagg cctgtcgcta
    28561 tgggctggaa ggtgtcatta ccgatccgca tactggcgat cgtcggtcac taacggaagc
    28621 caccttgcgt ttactggaaa aaatcgcccc ttctgcacat aaaattggcg catcgagcgc
    28681 aattgaagca ctgcaccgtc aggtcgtcag tggtctgaat gaagcgcagc taatgcgtga
    28741 tttcgtcgcc gatggtggct cgctaattgg gctggtgaaa aagcattgtg agatctgggc
    28801 cggagaataa gtcgggaatt gccttacaga agaatatccc tgacaatggt gttttaactt
    28861 acatttaaca atgttatgaa acacccttta gaaaccttga ccaccgcagc aggcattttg
    28921 ctgatggctt tcctctcttg cctgctgctg cccgcccccg cactggggct gacgctggca
    28981 caaaaactgg tgaccacgtt ccatctgatg gatcttagcc agctttacac tgtattgttt
    29041 tgtctgtggt ttttagtgct gggcgctatt gagtattttg ttctacgctt tatctggcga
    29101 cgctggttct cgctggcgga ttaaacgtcg attatcgccg aacttcgcgg catactttgc
    29161 ttattctcct tcgcctaaag gaacatttat ggataagcaa tcactgcacg aaacggcgaa
    29221 acgcctggct cttgagttac cctttgtcga gctttgctgg ccttttggcc cggagttcga
    29281 tgtttttaaa attggcggca agatttttat gctgtcgtcg gagctgtgcg gcgtcccctt
    29341 tatcaatctg aagtccgatc cacaaaaatc tctgttaaat cagcaaatat atccgagcat
    29401 taagcctggg tatcacatga acaaaaaaca ctggatttcg gtgtatcccg gcgatgaaat
    29461 ctccgaagcg ttacttcacg atctgatcaa cgattcatgg aatttagtga tcgatggtct
    29521 ggcgaagcgc gatcaaaaac gactgcgtcc tgactcaacc gtaaatcaat agcgcatttt
    29581 tctcgcttac catttcatct cgatccttgt aatctgctgg cacgcaaaat tactttcaca
    29641 tggagtcttt atggatatca tttctgtcgc cttaaagcgt cattccacta aggcatttga
    29701 tgccagcaaa aaacttaccc cggaacaggc cgagcagatc aaaactctac tgcaatacag
    29761 cccatccagc accaactccc agccgtggca ttttattgtt gccagcacgg aagaaggtaa
    29821 agcgcgtgtt gccaaatccg ctgccgataa ttacgtgttc aacgagcgta aaatgcttga
    29881 tgcctcgcac gtcgtggtgt tctgtgcaaa aaccgcgatg gacgatgcct ggctgaagct
    29941 ggttattgac caggaagatg ccgatggccg ctttgccacg ccggaagcga aagccgcgaa
    30001 cgataaaggt cgcaagttct tcgccgatat gcaccgtaaa gatctgcatg atgatgcaga
    30061 gtggatggca aaacaggttt atctcaacgt cggtaacttc ctgctgggcg tggcggcgct
    30121 gggtctggac gcggtgccca tcgaaggttt tgacgccgcc atcctcgatg aagaatttgg
    30181 tctgaaagag aaaggctaca ccagtctggt ggttgttccg gtgggtcatc acagcgttga
    30241 agattttaac gctacgctgc cgaaatctcg tctgccgcag aacatcacct taaccgaagt
    30301 gtaattctct cttgccgggc atctgcccgg ctatttcccc tcagattctc ctgatttgca
    30361 taaccctgtt tcagccgtca tcataggctg ctgttgtata aaggagacgt tatgcaggat
    30421 ttaatatccc aggttgaaga tttagcgggt attgagattg atcacaccac ctcgatggtg
    30481 atgattttcg gtattatttt tctgaccgcc gtcgtggtgc atattatttt gcattgggtg
    30541 gtactgcgga ccttcgaaaa acgtgccatt gccagttcac ggctttggtt gcaaatcatt
    30601 acccagaata aactcttcca ccgtttagct tttaccctgc aggggattat cgtcaatatt
    30661 caggcggtat tctggctgca aaaaggcacc gaagcggcag atattctgac tacctgcgcg
    30721 cagttgtgga tcatgatgta tgcgctgctt tcagtcttct cgttgctgga tgtgattttg
    30781 aatctggcgc agaaattccc ggcggcatct cagttaccgc tgaaagggat ttttcagggg
    30841 attaaactga tcggcgcgat tctggtcggc attttgatga tctcgctgct gattggtcag
    30901 tcgccagcga ttctgatcag cggtcttggt gcaatggctg ccgtgctaat gttggtattt
    30961 aaagatccga ttcttggtct ggtggcaggt attcagcttt ccgcgaacga tatgctgaaa
    31021 ctgggtgact ggctggagat gccgaaatac ggcgcggatg gcgcggtgat cgatattggg
    31081 ttaaccaccg tcaaagttcg taactgggac aataccatta ccactattcc cacctggtct
    31141 ctggtttctg actcctttaa aaactggagc gggatgtcag catctggcgg acgacgtatt
    31201 aaacgcagta tcagcattga tgtcaccagt attcgttttc ttgatgaaga cgaaatgcaa
    31261 cggctgaata aagcgcactt gttaaagcct tatttaacca gccgccatca ggaaattaat
    31321 gagtggaatc gccagcaagg ttctacggag tcggtattaa acctgcgccg aatgaccaat
    31381 attggaacct ttcgtgccta tctgaacgaa tatctgcgga atcatccgcg gattcgcaaa
    31441 gatatgacct taatggtacg ccaactggct cctggtgata acggtttacc gcttgagatc
    31501 tatgcgttta ccaacaccgt ggtgtggctg gaatatgaaa gcattcaggc tgatatattc
    31561 gatcacatat ttgcgattgt cgaagagttt ggtctgcgac ttcatcagtc gccaaccggc
    31621 aatgatattc gctctctggc gggtgcattt aagcagtaat taaaaaaacc gctctcatcg
    31681 aatagatgag agcggctttc agattgttga catcattttg tcggatgcgg cgtgaacgcc
    31741 ttatccgacc tacagaatcg ttacaaattg taggcccgat aagcttgcgc atcgggcaaa
    31801 tcagcctcac atcagcttaa ttcactttcc tacgcaatgt tttaaacgcc acaaacagga
    31861 acacaatcca caccggcagc agaatcgctg acaagcgcat atcatccatc gtgcacatca
    31921 gcagcaaaat catgccgagg aaggcgatgc aaagatagtt gccgaacgga tagagcagcg
    31981 ccttaaactg tgtttcacgc ccctgacgac gcatcgctgc acgaaaacgc agatgcgcca
    32041 ggcagatcat aatccagttc aacagcagcg ttgctaccac cagcgccatc agcagaccaa
    32101 acgctttttg cggcagcaga tagttgatta acaccaccag cgaagtgatc gctccggaaa
    32161 gcatcagcga gttaatcggc acgccgcgac ggctgacgcg agtcaaaaac tttggcgcat
    32221 taccctgcac agaaaggcca aacagcatgc ggctgttaga gtaaaccccg ctgttataca
    32281 ccgacagcga tgccaccaga atgacgaagt tcagcgcaga agccaccaca ttgctgtcga
    32341 gattatggaa aatcatcaca aacgggctac tgttggattt cacttccacc cacgggtaga
    32401 gcgccagtaa aaccaccagt gaaccgatat aaaacagcag gatgcgatac accacctgat
    32461 tcactgcttt agggatgctt ttttccggat cgcgcgcttc agccgcagta atcccaatca
    32521 gctccagacc gccgaaggag aacataatta ccgccagcga caaaatcaat ccattccagc
    32581 cggtggcaaa gaaaccgccg tagcgccaga ggttatcgat actggctttc tcaccgccgt
    32641 gcccagaaaa cagcagccac aggccaaagc cgatcatacc gatgatcgcc agcactttaa
    32701 tcagcgcaaa ccagaactcg gtttcgccat ataagcgcac gttcaccagg ttaacggcgt
    32761 tgatgataat aaagaaggcg gcagcccaaa tccacgttgg aacatccggg aaccagtact
    32821 gcatatagat gcccgcagcg gtcagctctg ccattcccac cagcacgaac attacccagt
    32881 agttccagcc agagaggaag cccgcaaacg gtccccagta tttataggca aagtgggcaa
    32941 atgaaccgga taccggctct tcgaccacca tctcgccgag ctggcgcata atcaggaaag
    33001 cgatgatccc ggcgacgccg tagcccagca atacagccgg acccgccatc tgaatcgccg
    33061 ggccaatgcc aagaaacaga ccagtaccaa ttgcgccacc cagcgcaatc agttgaatat
    33121 gacggttatg taatccgcga tgaagcgtcg gctcttgatt cgacgcagta tcttccgata
    33181 cggttgacgc gtttttcacg cctttcccct gtgtgtcttt tttgttgagg ggcacctttt
    33241 aacatttagt gcccatcgtc gcaagacaca atccacacgg ttaaacgggg tatcctgctt
    33301 ttatttccgt acccgatgtc ggtgcagcca catcagctta tacgccgccg ggataataaa
    33361 cagcgacagc aaaggcgcgg tgatcatgcc gccaatcatc ggcgcggcaa tccggctcat
    33421 cacctctgac cctgctcctg ttccccacag aatcggcagc agaccagcga taatcaccgc
    33481 caccgtcatc gcttttgggc gcacgcgcag taccgcgccg tgatataacg cctcatccag
    33541 cttctgctcg ctgaatgttt gcggattatt caacgacggc tcggcctcta tggcgtgacg
    33601 taaatacatc agcatcacca cgccaaattc ggcggcgacc ccggcaaggg caataaaacc
    33661 ggtgcccgtc gccacggaaa gatgaaagcc catccaccac agcaaccaga tgccgcccac
    33721 cagcgcaaac ggtacgctgc tgataatcag caacgcttcg cccacccgac ggaacgccag
    33781 atacaacagc acgaagatga tcatcaacgt catcggcacc atgagtttca gcttatggtt
    33841 ggcgcgctcc agtagctcga actgcccgga gaatgccacg ctggtgcccg gttttaactg
    33901 cactttttca gctatcgctt tttgcagatc gtgaaccacc gacaccatgt cacgatcgcg
    33961 ggcatcgata taaatccagc tcgtcgggcg cgcattctcg gttttcagca tcgacggtcc
    34021 ggtagagact ttaacgtcgg ccacgtctgc cagggtgatt tgctgcttca tcggcgtgag
    34081 gatcggcaac tggcgcagtg cctgcgggct atcgcgccag ctttgcggat aacgcagatt
    34141 aattggataa cgagcaatcc cttccaccgt ttcgccaacc atcgccccgc ccaccgcaga
    34201 agtcacaaac aactgcacat ccgccaccgt cataccgtaa cgcgcggctt tttcacggtt
    34261 aatctcaacg ttgatatagc gcccaccttc cagacgctca gcaagcgcag aagccacgcc
    34321 tggcaccgtt cgcgccactt cttcaatttg ctccgccatc gtgtcgatat ccgccagcac
    34381 agtgccggaa actttaatgc cgatagggct tttaatgcca gtcgagagca tatcgatacg
    34441 gttacgaatt ggcggcaccc acagattcgc cagccccggc agacgcacgg tgttatccag
    34501 ttcctcaatg attttgtcca tcgtcatgcc aggccgccac tgatcctgcg gcttaagctg
    34561 gatagtcgtt tctaccatct ccagtggagc tgaatcggtg gcggtttccg ctttcccggt
    34621 tttgccaaat acccgcgcca cttccggtac gctcataatc agcttgtcgg ttttttgcag
    34681 catgctcgcc gcctctgctg cggaaatccc cggcagcgtc gatggcatat acaacaagtc
    34741 gccttcattg atctgcggta aaaattcccc gccaacttta ttgagcggcc agagaaccgt
    34801 cagcaccgaa agcgctgcca ccaacagcgt ggttttcggc cagtgcagta ctttcagcaa
    34861 cagcggatga taaacacgaa tcaaaaagcg attgagcggg ttactgcttt ctggcggaat
    34921 tttgccacgg atccagtagc ccatcaggat cgggatcact acgatcgcca gcagcgccgc
    34981 gcccgccatc gcataggttt tggtgaacgc cagcgggcca aacagacgcc cctcctgccc
    35041 ttccagggtg aaaatcggga taaacgacag cgtgataatc agcagactga taaacagcgc
    35101 cggccccact tcaacagatg catcggtgat cacctgccag cgcgttttat tatccagcgt
    35161 ggcgtcagga tgctggtgct gccactcttc cagccgttta tgcgcattct cgatcatgac
    35221 gatagcggca tcgaccatcg ccccgacggc aatcgcaatg ccacccaacg acataatatt
    35281 ggcgttcagt ccctgaaagt gcatgacaat aaaagcaata cacaacccca gcggcaacga
    35341 aataatcgcc accagcgccg agcgcacatg ccagagaaac agcgcacaga ctaccgccac
    35401 cacaataaac tcttccagca acttgccgct gaggttgtca atggcgcggt caatgagctg
    35461 actgcgatcg tatgttgtaa ctatctccac gccttccggc agactgcttt tcagcgtttc
    35521 cagtttgtcc ttcacggcgg cgatcacttc acgggcgttt ttgccggaac gcaggatcac
    35581 caccccgccc gccacttcgc cttcgccgtt cagttcggca atgccccggc gcatctccgg
    35641 gccaacctgg accttcgcaa catcacgcag ataaacgggc acgccatttt cactggcttt
    35701 taaaacgatg tgattaaagt cgtcgagcgt ttgcagatag ccgctggcgc gcaccatata
    35761 ttccgcttcc gccagttcga tcgacgaacc gcccgcttcc tggtttgaag catccagcgc
    35821 gctttttact tcggcgagac tgatgccata ctgcgccagg cgctgaggat cgataaccac
    35881 ctgatactct ttcaccacac cgcccaccga cgccacttcc gcaacgtcag ggatggtttt
    35941 tagctcatat ttgagaaacc agtcctgcaa tgagcgtaaa tcggccagat cgtgcttacc
    36001 gctgcgatcc accagtgcat attcatagat ccagccaaca cccgtggcat ctggccccag
    36061 ctcggcgctg actcccgcag gcagcttacc ctgtacctgg ttgaggtact ccagcacccg
    36121 cgagcgcgcc cagtacggat cggtgccatc ttcgaaaatg acatacacat aagagtcgcc
    36181 aaactgcgag aaaccgcgca cagtcttcgc gccaggcacc gacaacatgg tggtggttag
    36241 cggataagtc acctgatttt caacgatttg cggtgcctga ccgggatagc tggttttaat
    36301 aatcacctgc acatcggaaa gatccggcag tgcatccact ggcgtgttaa tgatagtcca
    36361 ggtgccccag atactcagaa acaacgcgcc catcagcacc agaaaacggt tcgccaccga
    36421 gcgacgaata atccattcaa tcattggcta ttccctcaat gcgcatgggt agcactttca
    36481 gagcgcatcc gctccagtgc gccagaaata ttggcttcag aatcaatcag gaacaggccg
    36541 ctggaaacca ccttttcacc ttccgccaga ccagagcgta acgcggtgac gccttgcgat
    36601 gcctggaaaa cggcaacgcg tttcggtaca aagcgcccgt cagcatcaac ggtaatcacc
    36661 cgctgttcgt tgccggtatc aatcagcgcc tgtgacggaa tgagcagcat cggttcgctg
    36721 gcggtattga gttgcaacca ggcgttcatg cccggtttta gcgcctcgtc ggcgttgtcg
    36781 acttccagac gcagttgcag cgtgcgggtc gcagcatcca cgccaggtag cagcgtccat
    36841 ttgcggatgg tgagtgtttt atcaggtcgc gccggaacgg tgagcgtaaa ctgcgaggca
    36901 tctttcacca gccaggcgat ggactccggg atcgcagcag tgacccacac cgggtccata
    36961 ccctgaattt tcgcgaccac gttatctttg gcgatattca ttcccgcgcg cagatcaaac
    37021 gcggtgatca cgccatcaat gggcgctttg agcgtaaagc gagtctggat tttttgcgtg
    37081 gcgatcaggc ggcgaatatc agcctccggc attcccgcca gtcgcagtcg ctcaagaatg
    37141 ccttcagtct gggtcgccgt accgccggtt tcgcgcagca gtaaatactc actctgcgct
    37201 tccacccagt cgggaatggt caggtcgaga agcggcgcgc ccttttgcac tttatcgccc
    37261 acggtaagcg gatacacctt gtcgataaac ccggcggcgc gggcctgcac aatggcatac
    37321 tgatactcgt tgtaactgac attcgccggg aaactctggg aaaaagtcag cggcccgcgc
    37381 gtgacggtcg ccgttttcac ccccagattc tgcgtctgag tcgggtcaat gcgcacacca
    37441 gacgcagaac tctcttcatc ggcatatttc ggcaccagat ccatatccat aaacggcgat
    37501 ttccctggtt tatcgaaccg cgtattggga tacatcgggt cgtaccagaa taagacttta
    37561 cgttctgcgg tcgacgtttt ttctgcgggc ggttcctcct ttgcaaacca ggtaaaacct
    37621 gccgcagaaa taataccgcc cgcgatcatg ctgccgataa taagcgcgat ttttttcatc
    37681 tcattaaacc cggattactg gctgacttta atatcctgta ataaagaaag gctgccctgc
    37741 tggacaaaat taaacgccac tttgtcgccg gttttaattt cgctcatttt cgtctgcggg
    37801 gtgatggtaa agcgcatagt catctccggc cagttcacgg cagcaatcgg atcgtgatgg
    37861 atggtgattt ttttgctttc cagatcaaca ccttttacca cgccagtggc gctaataacc
    37921 tttggttgtg cttcgctcat ggtttcatga tgatgttcgt tagcctgggc attaaagcca
    37981 ataacggtaa acagactgaa cattgcgact tgcagtgttt ttttcattta ttctctcctg
    38041 gagttaaaaa ttattgctgc caaccgcccc ctaaggcggt atataaagaa atttcgttaa
    38101 cctgacgggc ataattcagg tcaagtaaag tttgtcgggt tgcaaataaa gaacgctcgg
    38161 catccagcac ttccagataa cttaccgcgc cgtgctgata taatgcccgc gcccgttgca
    38221 aagtaatttg cagcgacgca agataacgct gctgggcgct gatttgagcg ttcagggttt
    38281 gacgtaatgc cagcgcatct gccacttctt taaaggcgtt ctggactttc tgttcataat
    38341 tcaccaccga ctgctgctgg cgaatttcgg cgatatccag attggcctgg ttgcgtccgg
    38401 cattaaaaac ggggatctca attttaggaa taaaattcca catcccgctg ctggcgttaa
    38461 ataatgacga cagatcgctg ctagcggttg atattccgct ggtcaggctg atagacggga
    38521 aaaatgccgc acgcgcagca ccaatattgg cattagccgc cattaacgcg tgttcagctt
    38581 ccataatatc agggcgctgc aataagattt gcgacgacaa gcccgccggt aatttaacgc
    38641 tttgcaggct gtcgctgttt actgtctgcg cttgcggcag cttgccgtag cttcccaata
    38701 acagttgcaa tgcattattc gcctgcgcca gttccccctg acgtttagcg atgtcgctgc
    38761 gggtactttc tatcaccccg cgagcctgtt ccagcgccag aacattgctg ctaccggtca
    38821 acagttgttt ttcgacaaac gcatatgact gctgataatt acgcagcgtt tcttcggcta
    38881 tttgcaattg cgcatacgcc agttgctgat tgaaatagct ttgcgcgaca ttagaaacca
    38941 gcaaaatatg caccgcgcgc tgagcttcct cagtggctaa ataattttgg cgctcggctt
    39001 cgctcatgtt ctttaagcgc ccgaaaaaat cgagatcaaa gctggcgtta aggccagtcg
    39061 agaactcccg cgtcgtggct gaatcgcctt taagattgcc gctccagctg ccgctgccct
    39121 cgccattgag ctgtgggtag cggtcggcat cggtcagacg atattgcgcc cgcgcttcct
    39181 gcactttcag cgccgccatg cgcaaatccc ggttattcac caacgcctcg ctaatcagcg
    39241 tcttcacctg attatcaaca aaaaaggtgc gccagccagc gttctggtag ttatctgctg
    39301 cgttaaccag gccgttctgg ctgagtgaga actgctgcgg cacgggcatt gccggacgct
    39361 gataatccgg tgccagtgaa caaccggtta gcgcaagggc cacacaaaat ggcagaagtt
    39421 tacaaggaga cataggctca taatttctgg tgattttatg ccgccaactt tactcgccag
    39481 gctctgattt tccggtgaca ggaaaatgac aaaattgtca ttttgccaat aagcgattgc
    39541 catctgatcc cgctactcta gaattgcccg ggcaacatgc ggaggaaata tgaaactgtt
    39601 gattgtcgaa gatgaaaaga aaaccggaga atacttgacc aaagggttaa ccgaagccgg
    39661 ttttgtggtc gatttggccg acaacgggct gaatggctac catctggcga tgaccggtga
    39721 ttatgatctg ataatcctcg atattatgct gccggacgtg aacggctggg atatcgtgcg
    39781 catgttgcgt tccgccaata aggggatgcc gattctgttg cttaccgcgc ttggcaccat
    39841 tgaacatcgc gtcaaggggc tggagttggg ggcggatgac tatctggtga agccgttcgc
    39901 ttttgctgaa ctgctggcgc gggtgcgcac tctactgcgg cgcggggcgg cggtgattat
    39961 cgaaagtcag tttcaggttg ccgatctgat ggtcgatctc gtcagccgca aagtcacccg
    40021 cagcggcacg cgcatcactc tgaccagtaa agaatttact ctgctggagt ttttcctccg
    40081 ccatcagggc gaagtgctgc cccgctcgct tatcgcctcg caggtatggg acatgaattt
    40141 tgacagcgat accaacgcca ttgatgtggc ggtgaagcgg ctgcgcggca aaatcgacaa
    40201 cgactttgag ccaaagttga ttcagaccgt gcgcggcgtg ggttacatgc tagaggtgcc
    40261 ggatggtcag taagccattt cagcgcccgt tttcgctggc aacccgcctg acctttttta
    40321 tcagcctcgc caccatcgcg gcgtttttcg cctttgcatg gatcatgatc cactcggtaa
    40381 aagtgcattt tgccgagcag gatattaatg atttaaagga gattagcgcc acccttgaac
    40441 gggtacttaa tcaccctgac gaaacacaag cccgacgctt aatgacgctg gaagatatcg
    40501 tcagtggtta ttccaatgtg ttgatctccc tggcagatag ccacggtaaa acggtgtatc
    40561 actcccccgg tgcgccggat atccgcgagt ttacgcgtga cgccataccc gataaagacg
    40621 cgcagggcgg cgaggtgtat ctcctttccg gcccgacgat gatgatgcca gggcacggtc
    40681 acgggcatat ggaacacagc aactggcgga tgattaactt gccggttggc ccgctggttg
    40741 acggcaaacc gatttatacg ctctacatcg cgctttcgat cgattttcat cttcattaca
    40801 tcaatgattt gatgaataaa cttattatga ccgcatcggt aatcagcatc ctgatcgtct
    40861 ttatcgtgct gttggcggta cataaaggtc acgcgccgat ccgcagcgtc agccgtcaaa
    40921 tccagaatat cacctcgaaa gatctcgacg ttcgccttga cccgcagacc gtgcccattg
    40981 agctggaaca gctggtactg tcgttcaacc atatgatcga gcgtattgag gatgtcttta
    41041 cccgccagtc caatttctca gcggatatcg cccacgaaat tcgcacacct attactaatc
    41101 tcattacgca aacggaaatc gccctcagcc agtcgcgcag ccagaaggag ctggaagatg
    41161 tgctctactc taatctcgaa gaactgacgc gaatggcgaa aatggtcagc gatatgctgt
    41221 ttctcgctca ggccgataac aaccagctaa tccccgaaaa gaaaatgctc aacctggcgg
    41281 atgaagtcgg caaagtgttc gattttttcg aggcgttagc agaagatcgc ggcgtggagc
    41341 tgcggtttgt tggcgacgag tgtcaggttg cgggcgatcc gctgatgctg cgtcgggcgt
    41401 taagcaatct gctttctaat gccctgcgtt atacgccaac cggagagaca attgtcgtgc
    41461 gctgccagac ggtcgatcac ctggtgcaag ttaccgtcga aaaccccggt acgcccattg
    41521 cgcccgaaca cttaccgcga ttgtttgacc gtttctaccg cgttgatcct tcccgccagc
    41581 gaaaaggcga aggtagcggc attggtctgg cgatagtgaa atcgattgtt gtcgcgcata
    41641 aaggcactgt tgcagtaacg tcagatgcac gggggacaag gtttgttatc atattacccg
    41701 cttaatcctt cagcaaacgg caacttttat aaccagtgta aaaataacat gccgcaatag
    41761 taatacctat taatgataaa aagctgtcac aattcataaa aaaccttaat atacgccacc
    41821 ctaaacataa ccagcgttaa tgtaaggttt ttgtgtggac tggcttcttg atgtttttgc
    41881 tacctggctc tacggcttaa aagtaatcgc gataacgtta gcggtcatca tgttcatcag
    41941 cgggctggac gattttttta ttgatgtcgt ctactgggta cgccgcatta aacgcaagtt
    42001 gagtgtttat cgccgctacc cgcgaatgag ttaccgcgaa ctgtataaac cagatgaaaa
    42061 accgttagcg attatggttc cggcgtggaa tgaaacgggc gtcatcggca atatggccga
    42121 gctggcggcg accacgctcg actatgaaaa ctatcatatc tttgttggca cctaccccaa
    42181 cgaccccgat actcagcgtg atgttgacga agtgtgcgct cgcttcccga atgtgcataa
    42241 ggtagtctgc gcgcgtcctg gccccaccag caaagccgac tgcctgaaca acgtgctgga
    42301 cgccatcacc cagtttgagc gtagcgccaa tttcgctttt gctggtttta ttctgcatga
    42361 cgccgaagat gtgatttcac cgatggaatt gcgtctgttc aactatctgg tcgagcgtaa
    42421 agatctgatt cagatcccag tgtatccgtt cgaacgcgaa tggacgcact tcaccagcat
    42481 gacctacatt gatgagtttt cagaactgca tggcaaagat gttccggtgc gtgaagccct
    42541 cgccggacag gtgcccagcg caggcgtcgg cacctgtttc agccgccgcg ccgtgaccgc
    42601 actgttagct gacggtgacg gtattgcttt cgacgtgcag agtctgactg aagattacga
    42661 cattggcttc cgcctgaaag agaaaggtat gacggaaatt tttgtccgtt ttccggtggt
    42721 ggacgaagcc aaagaacgcg agcagcgtaa atttttacag catgcgcgga cgtcaaacat
    42781 gatctgcgtg cgcgaatatt tccccgatac cttttcgact gcggttcggc aaaaatctcg
    42841 ctggatcatc ggcattgttt tccagggctt taaaacccac aaatggacct ccagcctgac
    42901 gctgaactac tttctctggc gcgaccgcaa aggggcaatc agtaactttg tcagcttcct
    42961 cgcgatgctg gtgatgatcc agcttttgct gttgctggcg tatgaaagtt tgtggcccga
    43021 tgcctggcat ttcctttcta ttttcagcgg cagcgcatgg ttaatgactc tgctgtggct
    43081 aaactttggc ttgatgatta accgcatcgt gcagcgggtg attttcgtta ctggctacta
    43141 cggcctgacg caggggctgc tatctgtcct gcgtcttttc tggggcaacc tgattaactt
    43201 catggccaac tggcgcgcgt taaaacaggt acttcaacac ggcgatccac gtcgcgtcgc
    43261 gtgggataaa acaacgcatg acttccccag cgtgactggc gatacccgct cgttgcgtcc
    43321 gttaggccaa atcctgctgg aaaatcaggt catcactgaa gaacaactcg atacagcact
    43381 gcgtaatcgc gtcgaaggtc tacgcctggg cggttcaatg ctgatgcagg ggctgattag
    43441 cgccgagcaa ctggcacagg cgctggcaga gcaaaacggc gtggcgtggg aatccatcga
    43501 tgcctggcag atctcttcct cgctgattgc cgaaatgccg gcctccgtgg cgttgcatta
    43561 tgcggtactg ccgctgcgtc tggacaatga tgagttaatt gtcggcagtg aagatggtat
    43621 tgacccggtt tcgctggcgg ccctgacgcg taaagtcgga cgcaaagtgc gttacgtcat
    43681 tgttctgcgg ggacaaattg tcaccggatt acgccactgg tatgcacgcc gacgcggtca
    43741 cgatccgcgg gcaatgttgt acaatgcagt tcagcatcag tggctcacgg aacagcaggc
    43801 cggtgaaatc tggcggcaat atgtgccgca tcagttcctg ttcgccgaaa tactgaccac
    43861 gctcggtcat attaatcgtt cagcaattaa cgtgttgtta ttgcgccatg aacgcagttc
    43921 tctgccgctc ggcaagtttt tggtcaccga aggcgttatc agccaggaaa cgttggatcg
    43981 cgtcctgaca attcaacgcg aattacaagt ttcgatgcaa tcactattac tcaaagcagg
    44041 tttaaacact gaacaggttg cacaactgga gtccgaaaat gaaggagaat aaccttaatc
    44101 gcgtcatcgg atggtctggt ttactgctga catctttatt gagtaccagc gcactcgcag
    44161 acaatatcgg caccagcgca gaagagctgg ggctgagcga ttatcgccat tttgttattt
    44221 atccccgtct cgataaggcg ctgaaggcac agaaaaataa cgacgaagca accgccatcc
    44281 gcgaatttga atatatacac cagcaggtgc cggataatat tccgctgact ttataccttg
    44341 cggaagccta tcgccatttt ggtcatgatg accgggcgcg gctgttgctt gaggatcaac
    44401 tgaaacgtca ccctggagat gaccggcttg agcgcagtct ggcggctatt ccagttgaag
    44461 tgaaaagcgt tacgactgtt gaagaactgc ttgcccagca aaaagcgtgc gatgctgcgc
    44521 cgaccctgcg ttgtcgcagt gaagtcgggc agaatgccct gcggctggca caattacctg
    44581 tcgccagagc gcaactaaac gatgcgacgt ttgctgcatc gccggaagga aaaacgttgc
    44641 gaaccgatct gctgcaacgg gcaatctacc tgaaacaatg gtcccaggca gatacgctat
    44701 ataatgaagc acgccagcag aacacattaa gtgcggcaga acgccgtcag tggtttgacg
    44761 tgcttcttgc cgcgcagctg gatgatcgga tcctggcgct gcaatcacag gggatcttca
    44821 ccgatcctca gtcatatatt acttacgcga ccgcgctggc ttatcgtggc gaaaaagcac
    44881 gcctccagca ttatctcatt gaaaatacgc cgctatttac cacggacgca caagagaaaa
    44941 gttggctcta tctgttatct aaatacagcg gcaaccccgt tcaggcgttg gcgaattata
    45001 cggtacagtt tgccgacaac cgccagtatg ttgttggcgc gacgctaccg gtgctgttaa
    45061 aagaaggtca gtacggcgca gcgcaaaaac tgctcgccac cctccccgcc aatgaaatgc
    45121 ttgaggagcg ttatgccgtt agcgtagcga cccgtaacaa ggctgaagct ctgcgtctgg
    45181 cacgattgct gtatcagcaa gaaccggcaa atcttacccg cctggatcaa ctgacctggc
    45241 aactgatgca gaacgaacag tcacgcgaag ctgccgattt attgctgcaa cgctatcctt
    45301 tccagggcga tgcgcgtgtc agccagacgt taatggcgcg actggcgtct ctgctggaaa
    45361 gtcatcctta cctggcaacg ccggcgaagg tggcgatttt atcgaaaccc ttaccgctgg
    45421 cggagcaacg tcagtggcaa agtcagttgc cgggtattgc agataattgc ccggcaatag
    45481 ttcgcttgct gggcgatatg tcgccttcct acgatgctgc cgcctggaac cgtctggcaa
    45541 agtgttatcg ggacacgcta cccggtgtgg cgttgtatgc atggcttcag gccgaacaac
    45601 gacaaccgaa cgcctggcaa catcgtgcgg tagcctatca ggcgtatcag gttgaggact
    45661 acgccaccgc actggcggcc tggcagaaaa tcagtcttca cggcatgagc aatgaggatc
    45721 tgcttgctgc tgccaatacc gcccaggcgg caggaaatgg tgcggctcgc gatcgctggc
    45781 ttcagcaggc agaacaacgt gggctgggaa acaatgccct ctactggtgg ctgcatgcgc
    45841 aacgttacat tcctggtcag ccggaactcg cactgaacga tctcacgcgc tcaatcaata
    45901 ttgcgccttc tgccaacgct tacgttgcgc gggcgacaat ttatcgccaa cgtcataatg
    45961 tcccggcggc ggtgagtgat ttgcgcgccg cgctggaact ggaaccgaat aatagcaaca
    46021 cccaggcagc gctcggttac gccttgtggg atagcggtga tatcgcacag tcgcgggaaa
    46081 tgctcgaaca ggcacataaa gggctaccgg acgatccggc acttatccga caactggcct
    46141 acgtgaacca gcgtctggat gacatgcctg cgacgcagca ctacgcccgg ctggtgattg
    46201 atgacattga taatcaggcg ctgataaccc cactgacccc agagcaaaat cagcaacgct
    46261 tcaatttccg ccgtctgcat gaggaggtcg gtcgccgctg gacgttcagt ttcgattctt
    46321 ccatcggctt gcgttccggc gcaatgagta ccgctaacaa taatgtcggc ggcgcagcgc
    46381 cagggaaaag ctatcgtagc tacgggcaac tggaagccga gtaccgcatc ggacgcaata
    46441 tgctgctgga aggcgacctg ctctcggttt acagccgtgt atttgccgat accggtgaaa
    46501 acggggtgat gatgccggtg aaaaatccga tgtccggcac cggtctgcgc tggaagccgc
    46561 tgcgcaatca gatctttttc ctcgccgtcg aacagcagtt gccgctgaac gggcaaaatg
    46621 gcgcatccga taccatgctg cgcgccagcg cctcattctt taatggcggc aaatacagcg
    46681 acgaatggca cccgaacggt tcaggctggt ttgcccaaaa cctgtacctc gatgcggcgc
    46741 aatatgtccg ccaggatatt caggcgtgga cggcagatta tcgcgtaagc tggcatcaga
    46801 aggtggcgaa cggacagact attgagcctt acgctcacgt tcaggacaac ggttatcgtg
    46861 ataaaggcac tcagggcgcg cagcttggcg gtgtcggggt ccgctggaat atctggaccg
    46921 gcgaaacgca ctacgacgcc tggccgcaca aagtcagtct cggcgtcgag tatcaacata
    46981 cctttaaggc gattaatcaa cgtaacggag agcgcaacaa cgcgtttctc accattggag
    47041 tgcactggta aatgcgtaag ttcattttcg tattgctgac actgcttttg gtcagccctt
    47101 tttcctttgc gatgaaaggt attatctggc aaccacaaaa ccgtgatagt caggttaccg
    47161 atacccagtg gcaggggctg atgagtcagt tacgtttgca aggcttcgat accctggttt
    47221 tgcaatggac ccgttacggc gatgcattca cccagccaga acagcgcgcg ttattgttta
    47281 agcgtgcagc agctgcgcaa caggctggcc tgaagctgat tgtcgggctg aatgccgatc
    47341 cggaattttt tatgcaccag aaacagtcat ccgcggcgct ggaaagctat cttaatcgcc
    47401 tgctggctgc cgatctccag caagccagat tatggagcgc cgcgcctggc gtaacgccgg
    47461 atggctggta catcagcgcg gaaattgacg acctgaactg gcgcagcgaa gccgcccgtc
    47521 aacctttgct gacatggtta aacaacgcgc agcggttgat tagcgatgtt tcagcaaaac
    47581 cggtttacat cagtagtttt ttcgccggaa acatgtcgcc cgatggctat cgccaactgc
    47641 tggaacaagt taaaacaacc ggcgttaatg tctgggtaca ggatggcagc ggcgtggata
    47701 aactgaccgc tgaacagcgt gaacgttatc tacaggccag cgccgattgc caaagttccg
    47761 cccctgccag cggcattgtt tatgaactgt ttgtcgcccg caaaggcaaa acctttacag
    47821 cgaaaccgaa accggatgca gagatcgcct cgctgttagc gaaacgttcc tcttgcggta
    47881 aagacacgct ctatttctct ctgcgctatt tgcccgccgc gcagagtatc cttgagtatt
    47941 aatgattaaa gggagcaact tagcttgctc ctttcctttt cactttccta atcaaaatcc
    48001 tggcaagatg aattgtttag cagaatttct gttttcattt tattcgtctg aagtgttcca
    48061 attatttagc aaaaccgcga caacaaagtg atggtgtaat ttgtaaaagg gaacaaataa
    48121 atggcgagga aatggctcgc cctgcaggat tcgaacctgt agcccacgac ttagaaactc
    48181 caggaacgac attttaaatc aacaaattac cgcgccatct ctgcgttcac acgttccacc
    48241 acctcaaaat atgtaaaacc ttgcaagcca ttgcgaggcc ttatgtgtct cagttttgtt
    48301 cctctttttt atactaaaaa acacagcaat tgaggataaa cctcatgcta ttat
//
LOCUS       DAZFJL010000016        48183 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFJL010000016 DAZFJL010000000
VERSION     DAZFJL010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.016"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(292..1692)
                     /gene="asnS"
                     /locus_tag="ACHJ8C_000927"
     CDS             complement(292..1692)
                     /gene="asnS"
                     /locus_tag="ACHJ8C_000927"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO5550828.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1861..3063)
                     /gene="pncB"
                     /locus_tag="ACHJ8C_000928"
     CDS             complement(1861..3063)
                     /gene="pncB"
                     /locus_tag="ACHJ8C_000928"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO5550829.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLENKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3329..5941
                     /gene="pepN"
                     /locus_tag="ACHJ8C_000929"
     CDS             3329..5941
                     /gene="pepN"
                     /locus_tag="ACHJ8C_000929"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO5550830.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDVPLRLNGEDLKLVSVYINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDTSPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHADIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(5983..6750)
                     /gene="ssuB"
                     /locus_tag="ACHJ8C_000930"
     CDS             complement(5983..6750)
                     /gene="ssuB"
                     /locus_tag="ACHJ8C_000930"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO5550831.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARQALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(6747..7538)
                     /gene="ssuC"
                     /locus_tag="ACHJ8C_000931"
     CDS             complement(6747..7538)
                     /gene="ssuC"
                     /locus_tag="ACHJ8C_000931"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO5550832.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(7550..8695)
                     /gene="ssuD"
                     /locus_tag="ACHJ8C_000932"
     CDS             complement(7550..8695)
                     /gene="ssuD"
                     /locus_tag="ACHJ8C_000932"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO5550833.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEHVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRNVAQS"
     gene            complement(8692..9651)
                     /gene="ssuA"
                     /locus_tag="ACHJ8C_000933"
     CDS             complement(8692..9651)
                     /gene="ssuA"
                     /locus_tag="ACHJ8C_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO5550834.1"
                     /translation="MRKIIKLALAGLLSVSTLAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLTTFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     LSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(9644..10219)
                     /gene="ssuE"
                     /locus_tag="ACHJ8C_000934"
     CDS             complement(9644..10219)
                     /gene="ssuE"
                     /locus_tag="ACHJ8C_000934"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO5550835.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYVREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSTLKAQEILHGVFADDSQVIDYQHKPKFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            10456..11466
                     /gene="pyrD"
                     /locus_tag="ACHJ8C_000935"
     CDS             10456..11466
                     /gene="pyrD"
                     /locus_tag="ACHJ8C_000935"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO5550836.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQVMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            11640..12182
                     /gene="zapC"
                     /locus_tag="ACHJ8C_000936"
     CDS             11640..12182
                     /gene="zapC"
                     /locus_tag="ACHJ8C_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO5550837.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWMPMPGDAAYVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(12179..13288)
                     /gene="ycbX"
                     /locus_tag="ACHJ8C_000937"
     CDS             complement(12179..13288)
                     /gene="ycbX"
                     /locus_tag="ACHJ8C_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO5550838.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPLMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVVRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAGA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAIGDDGTILCCSCVPKTALKPAR"
     gene            13386..15494
                     /gene="rlmKL"
                     /locus_tag="ACHJ8C_000938"
     CDS             13386..15494
                     /gene="rlmKL"
                     /locus_tag="ACHJ8C_000938"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO5550839.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPVMAA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            15506..17413
                     /gene="uup"
                     /locus_tag="ACHJ8C_000939"
     CDS             15506..17413
                     /gene="uup"
                     /locus_tag="ACHJ8C_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5550840.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELELLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            17543..18796
                     /gene="pqiA"
                     /locus_tag="ACHJ8C_000940"
     CDS             17543..18796
                     /gene="pqiA"
                     /locus_tag="ACHJ8C_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO5550841.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            18801..20441
                     /gene="pqiB"
                     /locus_tag="ACHJ8C_000941"
     CDS             18801..20441
                     /gene="pqiB"
                     /locus_tag="ACHJ8C_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO5550842.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     LLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            20438..21001
                     /gene="pqiC"
                     /locus_tag="ACHJ8C_000942"
     CDS             20438..21001
                     /gene="pqiC"
                     /locus_tag="ACHJ8C_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO5550843.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVHSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            21257..21424
                     /gene="rmf"
                     /locus_tag="ACHJ8C_000943"
     CDS             21257..21424
                     /gene="rmf"
                     /locus_tag="ACHJ8C_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO5550844.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(21494..22012)
                     /gene="fabA"
                     /locus_tag="ACHJ8C_000944"
     CDS             complement(21494..22012)
                     /gene="fabA"
                     /locus_tag="ACHJ8C_000944"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO5550845.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTAND
                     LKVGLFQDTSAF"
     gene            complement(22081..23841)
                     /gene="ycbZ"
                     /locus_tag="ACHJ8C_000945"
     CDS             complement(22081..23841)
                     /gene="ycbZ"
                     /locus_tag="ACHJ8C_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5550846.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGGHYEVSGHTIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWSVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEDKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            24027..24479
                     /gene="matP"
                     /locus_tag="ACHJ8C_000946"
     CDS             24027..24479
                     /gene="matP"
                     /locus_tag="ACHJ8C_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO5550847.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDEL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(24556..25608)
                     /gene="ompA"
                     /locus_tag="ACHJ8C_000947"
     CDS             complement(24556..25608)
                     /gene="ompA"
                     /locus_tag="ACHJ8C_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO5550848.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQA
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(25964..26473)
                     /gene="sulA"
                     /locus_tag="ACHJ8C_000948"
     CDS             complement(25964..26473)
                     /gene="sulA"
                     /locus_tag="ACHJ8C_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO5550849.1"
                     /translation="MYTSGYAHRDSSFSSTASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTAEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            26692..27321
                     /gene="sxy"
                     /locus_tag="ACHJ8C_000949"
     CDS             26692..27321
                     /gene="sxy"
                     /locus_tag="ACHJ8C_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO5550850.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     CLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(27284..29446)
                     /gene="yccS"
                     /locus_tag="ACHJ8C_000950"
     CDS             complement(27284..29446)
                     /gene="yccS"
                     /locus_tag="ACHJ8C_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO5550851.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNDAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRIQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVLA"
     gene            complement(29456..29902)
                     /gene="yccF"
                     /locus_tag="ACHJ8C_000951"
     CDS             complement(29456..29902)
                     /gene="yccF"
                     /locus_tag="ACHJ8C_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO5550852.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFQ"
     gene            30025..32079
                     /gene="helD"
                     /locus_tag="ACHJ8C_000952"
     CDS             30025..32079
                     /gene="helD"
                     /locus_tag="ACHJ8C_000952"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO5550853.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGMQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIEGARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDEGQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVDILKNLDVPVAR
                     KP"
     gene            complement(32111..32569)
                     /gene="mgsA"
                     /locus_tag="ACHJ8C_000953"
     CDS             complement(32111..32569)
                     /gene="mgsA"
                     /locus_tag="ACHJ8C_000953"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO5550854.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(32665..33327)
                     /gene="yccT"
                     /locus_tag="ACHJ8C_000954"
     CDS             complement(32665..33327)
                     /gene="yccT"
                     /locus_tag="ACHJ8C_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO5550855.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            33500..33913
                     /gene="yccU"
                     /locus_tag="ACHJ8C_000955"
     CDS             33500..33913
                     /gene="yccU"
                     /locus_tag="ACHJ8C_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO5550856.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLAEVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(33958..34275)
                     /gene="hspQ"
                     /locus_tag="ACHJ8C_000956"
     CDS             complement(33958..34275)
                     /gene="hspQ"
                     /locus_tag="ACHJ8C_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO5550857.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(34333..35523)
                     /gene="rlmI"
                     /locus_tag="ACHJ8C_000957"
     CDS             complement(34333..35523)
                     /gene="rlmI"
                     /locus_tag="ACHJ8C_000957"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO5550858.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPNESIDIAFFTRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPITGELPPALLPIEEHGMKLLVNIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            35618..35896
                     /gene="yccX"
                     /locus_tag="ACHJ8C_000958"
     CDS             35618..35896
                     /gene="yccX"
                     /locus_tag="ACHJ8C_000958"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO5550859.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLAGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIH"
     gene            complement(35893..36222)
                     /gene="tusE"
                     /locus_tag="ACHJ8C_000959"
     CDS             complement(35893..36222)
                     /gene="tusE"
                     /locus_tag="ACHJ8C_000959"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO5550860.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(36313..36972)
                     /gene="yccA"
                     /locus_tag="ACHJ8C_000960"
     CDS             complement(36313..36972)
                     /gene="yccA"
                     /locus_tag="ACHJ8C_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO5550861.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLATGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(37382..38401)
                     /locus_tag="ACHJ8C_000961"
     CDS             complement(37382..38401)
                     /locus_tag="ACHJ8C_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5550862.1"
                     /translation="MGRPRKDKKDNVLPPRVRSNGYSYVWKPKGSTRTIKLGRVRETS
                     VAKVWQNYELEKAKLHDIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQRALLAVFGK
                     MLADNVKIEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKTNPCKGVRKFT
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSGTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKAGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(38370..38621)
                     /locus_tag="ACHJ8C_000962"
     CDS             complement(38370..38621)
                     /locus_tag="ACHJ8C_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO5550863.1"
                     /translation="MYELTLSPAEIQEITKYKRYTRQQHQLRLHGIPFLIGPKNEPIV
                     LRCDIPRGLTAMSKVSELVSADPDFEALNNGKTKKRQKR"
     gene            complement(38688..41168)
                     /locus_tag="ACHJ8C_000963"
     CDS             complement(38688..41168)
                     /locus_tag="ACHJ8C_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO5550864.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAIAVATAIEAGDERRARAKFTWQF
                     LEQYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEDSASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQHTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATDTLSSPVPETDAVETAIPDNEKTECKVEIEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLNKALTAGATEITKEEYFARKNGTYSGLQQNTGASDTTAQ
                     PEPVKVTADEVKKIMQAANISQPDANQLLAASRGEFVAGISDPNDPKWVKGIETRDSV
                     NQNQQETEQNDQKAEQNSQNALQNEPETKQPEPVAQQEPEKVCTACGQSGGGNCPDCG
                     AVMGDATYQETFDEENQVEVQENDPEEMEGAEHPHKENAGSAQDHASDNETGETAAPL
                     IAVNGHHIITSTSRVWYHLMIDLETMGTNTNAPIVVIGAVFFDPQTGEIGPVFYIVIS
                     LTDAMNTGAVPDGGTIEWWLKQSSEARAAILTDQVKLKDALSRFREFINEYSDEKFVQ
                     VWGNGATFDNSILRTSYERLGIPCPWHYSNDRDVRTIVELGKTIDFNARSVIPFEGVP
                     HNALDDALHQAKYVTATIQKLIPNQADF"
     gene            complement(41292..41513)
                     /gene="kil"
                     /locus_tag="ACHJ8C_000964"
     CDS             complement(41292..41513)
                     /gene="kil"
                     /locus_tag="ACHJ8C_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO5550865.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVIHKGRTYIASVNNIKKQRLYIR
                     DFSTQHCIKETMIKVFLGRDGLPVKAESW"
     gene            42080..42358
                     /locus_tag="ACHJ8C_000965"
     CDS             42080..42358
                     /locus_tag="ACHJ8C_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5550866.1"
                     /translation="MDKKQIEALQSIIEKQDEAIRILSYRTDMILNMLSALTAALGGT
                     KTNVYREVVIQQIDKFEKTIPGINAHLAEQEKDHALMAISSVALPKVE"
     gene            complement(42318..42719)
                     /locus_tag="ACHJ8C_000966"
     CDS             complement(42318..42719)
                     /locus_tag="ACHJ8C_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5550867.1"
                     /translation="MEFKDLPMPFQEMAANIVRSQLATLDLSTVEKETIDTISGNVRR
                     AFIGLYEEKRPFGGQNSPENKNQANDEKLKHIIALLLEDAKRLQQLEPNAGTEARIWI
                     AMKSLKCESSDYFKTTIKTTQLSGELLKKLP"
     gene            complement(42735..42950)
                     /locus_tag="ACHJ8C_000967"
     CDS             complement(42735..42950)
                     /locus_tag="ACHJ8C_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5550868.1"
                     /translation="MQKPEPIIIAPGYTDDEIYEWMCGKIRAINDLKQAADYKERLSK
                     ELASAEQDITTLAKSAALNVSRVIENY"
     gene            complement(42980..43108)
                     /gene="ydfB"
                     /locus_tag="ACHJ8C_000968"
     CDS             complement(42980..43108)
                     /gene="ydfB"
                     /locus_tag="ACHJ8C_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO5550869.1"
                     /translation="MDFDAIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSND"
     gene            complement(43110..43265)
                     /locus_tag="ACHJ8C_000969"
     CDS             complement(43110..43265)
                     /locus_tag="ACHJ8C_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO5550870.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARRW
                     LERNSGE"
     gene            complement(43586..44062)
                     /gene="racR"
                     /locus_tag="ACHJ8C_000970"
                     /gene_synonym="rarC"
     CDS             complement(43586..44062)
                     /gene="racR"
                     /locus_tag="ACHJ8C_000970"
                     /gene_synonym="rarC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor RacR"
                     /protein_id="HFO5550871.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGAVKSKAEIARHFKVQPPSIHDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPSCSDSKRELLDINSLYQAASD
                     EKRAIVAFLLSGNAAEPGWVDYDVRAYITAMEMKVSNYLKNQESERKSQNITKTGT"
     gene            44187..44510
                     /locus_tag="ACHJ8C_000971"
     CDS             44187..44510
                     /locus_tag="ACHJ8C_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5550872.1"
                     /translation="MKKENYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRRLPA
                     ERCPAIERATKGGVLCEELRPDVDWSYLRRSSCYSQNMSMKQPNDKNDHTRSIKRQMI
                     HENQA"
     gene            44494..44919
                     /locus_tag="ACHJ8C_000972"
     CDS             44494..44919
                     /locus_tag="ACHJ8C_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO5550873.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAEITRAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPPLVAWMRSHSSDYFRE
                     LVDTHERLVRDADELVAVVIAGFNQMNRGGPAGNAVVAH"
     gene            44941..45906
                     /locus_tag="ACHJ8C_000973"
     CDS             44941..45906
                     /locus_tag="ACHJ8C_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5550874.1"
                     /translation="MKLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTGSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     ESELLDGGKVSESIRSKCTAPSGQNDTMEEVKMARSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTSRPEASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLACAQWIWGR
                     IVSLYEQAASDDGEISRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            45913..46659
                     /gene="ydaV"
                     /locus_tag="ACHJ8C_000974"
     CDS             45913..46659
                     /gene="ydaV"
                     /locus_tag="ACHJ8C_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5550875.1"
                     /translation="MKNIATGGVLERIRKLTPQHVTAPCRTVDEWREWQLAEGRKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQAVIVVTVADVMSALHASYDDGQSGEKLLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVSFNWESWRPNVVQPGITK"
     gene            46682..47428
                     /locus_tag="ACHJ8C_000975"
     CDS             46682..47428
                     /locus_tag="ACHJ8C_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO5550876.1"
                     /translation="METVFDALKAMGKATSIELAARLDVSREEVLNELWELKKAGFVD
                     KSAYTWRVADNNVQQEQPAQAELPEETTTATVAKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQNGKFRYCMPDDNLPAKPKAVSVTETDGKAFPQ
                     PAGVALPVQKDATQEDIKTETVADIVQSLPSFTATREDDLILPSLHMANRELRRAKNH
                     VQKWERVCAALRELNKHRDMVAGICRKSGQ"
     gene            47425..47589
                     /locus_tag="ACHJ8C_000976"
     CDS             47425..47589
                     /locus_tag="ACHJ8C_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5550877.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKSRVKGISLILR
                     GDFHQSTKYP"
     gene            47594..48127
                     /locus_tag="ACHJ8C_000977"
                     /pseudo
     CDS             47594..48127
                     /locus_tag="ACHJ8C_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489808.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT        12202 a        11775 c        12396 g        11810 t
ORIGIN      
        1 taaacggaaa ttttgtttct gatggaaact ttatcgacca ggcacaaaat cttctttcag
       61 acatccagaa tgccaaaaat ttgtatgaaa attgtgctat cagctaagaa aaaactaatc
      121 cgcaataaga atgcagatta ttttttgtgc gtaaatgttg cagcgtaata ttccgtaaca
      181 ggatgatcgt tatggcgggg ttgcaaatag attgcttgac ttaacaaaca gataacttga
      241 cagaaaggat aaaaaaagag ccagcgggcg ctggctcttg aagacgctaa attagaagct
      301 ggcgttacgc ggagtacgtg ggaacggaat aacatcacgt acgttttgca cgccagttac
      361 gtaagcaatc aggcgttcaa aaccaagacc gaaacctgaa tgcggaacag taccgtagcg
      421 acgcagatcg cgataccacc agtaatcttc tttgttcaga cccatttcaa gcatacgctc
      481 gtccagcacg tccagacgtt cttcacgctg ggagccacca atgatctcac cgatgcccgg
      541 agccagaacg tccatagccg caacggtttt accgtcttcg ttaaggcgca tatagaacgc
      601 tttaatatct ttcggatagt ttttaaccac taccggtgct ttaaagtgtt cttccgccag
      661 ataacgctca tgctcagaag agagatcgac gccccagtaa accgggtttt caaacttcct
      721 gccgcagttt tcgagaatgg tcactgcgtc ggtgtaatcc acctgcgcga aatcggcctc
      781 aatgaagcgt tccagacgtg aaacggcatc tttatctacg cgttcagcga agaatttcat
      841 gtcgtcagcg cgttcttcga gaaccgcttt gaagacatat ttcagcatgg cttcagccag
      901 acctgcgata tcgttcaggt tagcaaacgc cacttccggc tccagcatcc agaattccgc
      961 caggtgacgg ctggtgttgg agttttcagc acggaaagtc gggccgaagg tataaatttt
     1021 ggacaatgcg caagcgtagg tttcgccgtt caactggcca gatacggtca ggaaagactc
     1081 tttaccaaag aagtctttgt cgaaatccac tttgccctga tcgttacgcg gcaggttttc
     1141 cagatccagc gtagaaacac ggaacatttc gcctgcacct tcggtatcag atgcggtaat
     1201 cagtggcgtt gaaacccaga agaatccctg ctcgttaaag aagcgatgca gcgcctgcgc
     1261 cagcgtatgg cgaacgcgcg cgacggcacc aatcaggttt gtgcgcggac gcaggtgagc
     1321 gacttcacgc agatactcaa tgctgtggcg ttttgccgcc atcgggtaag tgtctggatc
     1381 ttcaacccaa ccagcaactt caaccttgct ggcctgaatt tcaaattgtt gcccctggcc
     1441 cggcgacgcc acgactttac ccgtcacaat gaccgagcag ccggtggtca gacgcaagac
     1501 gtcttcattg taattgggca gagaattatt gatgacagcc tgtacaggat caaagcagga
     1561 accgtcataa acggcgagga aggagatgcc agcttttgaa tctcggcggg tacgtaccca
     1621 tccgcgcacg gtgacttcgc tgtcaacggc tacgcggccc tggagtacgt cggctacagg
     1681 cacaacgctc ataatattct ctctgttaat agtcggaaaa aataaacact tgtccacccg
     1741 taaatggggg gatacctatg ttacctggca tctgcaatca gacaagcaga attcgcaaat
     1801 gcagcgaaag atttcggaat taaaggtaaa aaaagggagc cgattagctc cctgatgata
     1861 ttaactggct tttttaatat gcggaaggtc gaacgctttg cgcagcgccc gaacaaacgc
     1921 tttatcgtgg cagatagttt tgccagggct gtcagaaagt ttcgccactg gtttaccgtt
     1981 acactctacc aacttaatga caatattcag gggttttacc tgggggatat cgcaggtcag
     2041 gcgagtccca ataccaaaac ttaattgcac gcgggaagag aagtggcggt atagctcaac
     2101 cgctttgcgt aaatccagat tgtcagagaa aaccagcgtt ttactctgtg gatcaattcc
     2161 cagcttttca taatgtgcaa tggctttttc accccattca accgggtcgc cagagtcatg
     2221 gcgcaggccc tggtaacgac tggcaaattc cacaccgaaa tcacgcagga aagcatccat
     2281 agtgatgcag tcggttaacg caatgccaag ttggtcggga tactcttcca gccaggcagc
     2341 aagtgcagct cgctggctgt tggctagatc cgggctgatt tgctgatgcg cctggaacca
     2401 ttcgtgtgcc tgtgttccca tcggcgtgag ggaaagccga cgcgccagat cgtagttgct
     2461 ggtgcccacg aaccaggatt cctgttgcag acgtttcacg atggtttctt gtacttcgcg
     2521 agaaaaacgg cggcgggtgc caaaatccat cagatggaag cgcgacatat caagaccggc
     2581 ggttaatgcc gagaagtcga ctaatttgtt ttccagcgtg tcgagggctt gcgcaacgtc
     2641 ggcctgcggt gagcgatagc gatgtaccat ttcactgata accgccagca aaggaacttc
     2701 ccagaggatg acttcacgcc acgggccgct taaacgaata tccagcttgc cattatcgtt
     2761 ggacacggtg acttgttccg ggttaaagcg gaactcgcgt agccagttaa gatagtcggc
     2821 cttaaagaaa ggcagggcag agagccactg atattcatca tcctgcaggc gcaggtgctg
     2881 catcgcctga acctgttcac gaatagcatc ggcataaata cccagcagat cgtcacctcg
     2941 gcaacgaaac tccgccgcga catgcacatc gtaatagtga tgaaacacgg cttgctgcat
     3001 atgcaactta taagcatctg tatccagcaa cgagtgcaga acaggagaag cgaattgtgt
     3061 cataggtgcg cagtagcgtc ctcgtacagg agcgtttagt acaataaaca tcttcatgaa
     3121 ctgctggagt ataccttgtt tcgcaattta ttgaaccccg atcacaccat atgccacctt
     3181 tctggtcgat ggcataacgc ggtgtatgtt ataaaaatgt agcaataaag gcgtttgtac
     3241 ctgaaaagat gaagattctg catagcgcga tttacgcaac aggaatagac tgaacaccag
     3301 actctataaa agatgctaaa ggttatttat gactcaacag ccacaagcca aataccgtca
     3361 cgattatcgt gcgccggatt accagattac tgatattgac ttgacctttg acctggacgc
     3421 gcaaaagacg gtcgttaccg cggtcagcca ggctgtccgt catggtgcat cagatgttcc
     3481 gcttcgtctc aacggcgaag acctcaaact ggtttctgtt tatattaatg atgagccgtg
     3541 gaccgcctgg aaagaagaag agggcgcact ggtcatcagt aatttgccgg agcgttttac
     3601 gcttaagatc gttaatgaaa ttagcccggc ggcgaatact gcgctggaag ggctttatca
     3661 gtcaggcgat gcgctttgca cccagtgtga agccgaaggt ttccgccata ttacgtatta
     3721 tctcgaccgc ccggatgtgc tggcgcgttt taccaccaaa attattgccg ataaaaccaa
     3781 atatcccttc ctgctttcca atggtaaccg cgttgcgcaa ggtgaactgg aaaatggacg
     3841 tcattgggta cagtggcagg acccgttccc gaaaccgtgt tacctgtttg cgttggtggc
     3901 tggtgatttt gatgtactgc gcgatacctt cactacccgc tctggccgtg aagtagcgct
     3961 ggagctgtac gtcgatcgtg gcaaccttga tcgcgcgccg tgggcgatga cctcgctgaa
     4021 aaactccatg aaatgggatg aagaacgctt cggcctggag tatgacctcg acatctatat
     4081 gatcgtcgcg gtggatttct tcaatatggg cgcaatggag aataaggggc tgaatatctt
     4141 taactccaaa tatgtgctgg cccgcaccga caccgccacc gacaaagatt acctcgatat
     4201 tgaacgcgtt atcggccatg aatatttcca taactggacc ggcaaccgtg tcacctgccg
     4261 cgactggttc cagctcagtc tgaaagaagg tttaaccgtc ttccgcgatc aggaattcag
     4321 ctctgacctt ggttcccgcg cggtaaaccg tatcaataat gtgcgcacca tgcgcggatt
     4381 gcagtttgca gaagacacca gcccgatggc gcacccaatt cgcccggata tggtcattga
     4441 aatgaacaac ttctacaccc tgaccgttta cgagaagggc gcggaagtga ttcgtatgat
     4501 ccacactctg cttggcgaag aaaatttcca gaaagggatg cagctctatt tcgagcgtca
     4561 tgatggtagc gcagcgacct gtgacgattt tgtgcaggcg atggaagatg cgtcgaatgt
     4621 cgatctttcc catttccgcc gttggtacag ccagtccggc acgccggttg tgaccgtcaa
     4681 agacgactac aatccggaaa ctgagcagta caccctgacc atcagccagc gcacgccagc
     4741 tacaccggat caggcagaaa aacaaccact gcatattccg tttgccatcg aactgtatga
     4801 caacgaaggc aaagtgatcc cgttgcagaa aggcggtcat ccggtgaatt ccgtgctgaa
     4861 cgtcacccag gcggaacaga cctttgtttt tgataatgtc tacttccagc cggtgcctgc
     4921 gctgctgtgc gaattctctg cgccagtgaa actggaatat aagtggagcg atcagcaact
     4981 gaccttcctg atgcgtcatg cgcgtaatga tttctcccgc tgggatgcgg cgcaaagcct
     5041 gctggcaacc tacatcaagc tgaacgtcgc gcgtcatcag caaggtcagc cgctgtctct
     5101 gccggtgcat gtggctgatg ctttccgcgc ggtactgctt gatgagaaga ttgatccggc
     5161 actggcggca gaaatcctga cgctgccttc cgtcaatgaa atggcagaac tgttcgatat
     5221 catcgacccg attgctattg ctgaagtacg cgaagcactc actcgtactc tggcgactga
     5281 actggcggat gagctactgg ctatttacaa cgcgaattac cagagcgagt accgtgttga
     5341 gcatgcagat attgcaaaac gcactctgcg taatgcctgc ctgcgtttcc tcgcttttgg
     5401 tgaaacgcat ctggctgatg tgctggtgag caagcagttc cacgaagcaa acaatatgac
     5461 tgatgcgctg gcggcgcttt ctgcggcggt tgctgcacag ctgccttgcc gtgacgcgct
     5521 gatgcaggag tacgacgaca agtggcatca ggatggtctg gtgatggata aatggtttat
     5581 cctgcaagcc accagcccgg cggcgaatgt gctggagacg gtgcgtggcc tgttgcagca
     5641 tcgctcattt accatgagca acccgaaccg tattcgttcg ttgattggtg cgtttgcggg
     5701 cagcaacccg gcagcgttcc atgccgaaga tggcagcggt taccagttcc tggtggaaat
     5761 gcttaccgac ctcaacagcc gtaacccgca ggtggcttca cgcctgattg agccgctgat
     5821 tcgcctgaaa cgttacgatg ccaaacgtca ggagaaaatg cgcgcggcgc tggagcagtt
     5881 gaaagggctg gaaaatctct ctggcgatct gtacgagaag atcaccaaag cactggctta
     5941 aaaaataacc gaatggcggc aatagcgccg ccattcggga atttacccct gttttctcag
     6001 gcgagtttca gattcaccac gctgcatcac ccgttgcaac acttccgctt ccagctccgc
     6061 taatcgcacc gaccctaacc ggcgcggacg ggggatatcc accgtcaaat ccagaccaat
     6121 ttttccctct tcaattaaca acacccggtc agccatcgcc accgcttcgc tcacatcatg
     6181 cgtcaccagc aagacggtaa agccgtgctc ctgccagagt gacacaatca aatcctgcat
     6241 ctcgagtcgc gttaaagcgt ccagcgcacc gagtggttca tcaagcaaca ataaacccgg
     6301 tcgatgaatc agcgcccttg ccagcgccac acgttgtttc tgcccgccag aaagtgcggc
     6361 aggccattcc cccgcgcgat tctccagccc taccgcagcc agagcctgac gcgccgcatc
     6421 gcgccactgg cctttaaggc ctaacccaac gttatcaatc accgatttcc acggcagcag
     6481 acgcgcgtcc tgaaacatca ttcgcgtatc atcctgaatc tcagccagcg gtgtggtgcc
     6541 cgctaacacg tcgcctgcgg ttggcgtttc cagacctgcc agcaggcgca gcagggtact
     6601 tttgccaccg ccgctgcggc ccaccaccgc cacaaactga cctgccggaa tatgtaaatc
     6661 cagttggttc aggacgatat tttccgcgta atgtttgctt actgcattga gcaacaatgg
     6721 tgtgccctgg ttcagacgag cagtattcat accgtggcct ccttcaaatg ataagccggg
     6781 ttccagcgca accagagacg ttctaacagc tgcgcgctga cgtcagcaag tttgccgagc
     6841 agggcgtaaa gaataatagc gactaccacc acgtccgttt gcagaaactc ccgcgcattc
     6901 atcgccagat aaccaatgcc ggaattggca gaaatagttt cggcaacgat cagcgtcagc
     6961 cacatcaggc ccaacgcaaa acgcacaccg accataattg agggcagggc accaggcagg
     7021 atcacatgga taaacagcgg tatgccggat aatccatagc tacgtgccat ctccaccagc
     7081 ccacggtcga tattacggat gccatgccag gtgttgatat aaatggggaa tagcgtaccg
     7141 agcgccacca gaaagatttt tgcggattca tcaatgccaa accacaaaat caccagcggg
     7201 atcagcgcca gatgcggcac gttgcgcaac atctgaattg aggtatccag cagtcgctca
     7261 ccccagcgtg acagcccgct aatcagcccc agaattaatc ccagcgatcc gccaattgaa
     7321 aagccaatca acgcccgcca ggagctgatc gccagatgct gccacaattc gccgctggca
     7381 gagagcgtcc agaacgccat caccactcct tccggtgagg gcaaaatacg cgtcgatagc
     7441 cagccaaccg aggaggccag ttgccacacc gccacgatgc ccaccggtaa aaaccaggga
     7501 gcaactcgca ataaccactt cttcactggc gttgccatca tcgtgttcct tagctttgcg
     7561 cgacgttacg ggggataaaa tcattcgcca ccgcttcgcc ttgtggattc agcggttgtg
     7621 gctggggaat ttccgggatg gcgacatcca gatgtgggaa cagtaactcg ccgacccgat
     7681 acgcttcttc cagatgcggg tagcccgaaa gcacaaaact gtcgatgcca agtgcagcgt
     7741 attcgttgat tcgcgcggcg accgtaggac catcgcccac cagcgccgtc ccggcaccgc
     7801 cgcgcactaa gccaacaccc gcccataaat tggggctgat ctccagatta tcgcgcttgc
     7861 cgttatgtag cgccgccatt cgctgttgtc ctacggaatc cgttcgggca aacgccgcct
     7921 gtgctttggc gatagtttca tcatcaagat gcgagattaa ccgctcggcg gcctgccacg
     7981 cttcgtcgtt ggtttcacga acaatcacat gcagacgaat accgaaacga attttgcgtc
     8041 catgcgcggc agctttcgcc cgcacgtgtt cgattttctc ttttaccagt tccggtggtt
     8101 cgccccaggt aaggtagaga tcaacttgtt ctgccgccag ctcctgggcg acatctgacg
     8161 atccgccaaa gtaaagcgga ggataaggct gttgaatcgg cgggaagagc agtttggctc
     8221 cgcgtacatg aatatgtttg ccgttgaaat ccactgtctc gccaagcaac aaccgccgcc
     8281 agacctgggt aaattctgcc gaggcttcgt aacgctcgct atgatcaagg aacacgccgt
     8341 cgcctgccag ttcttgggga tcgctgcctg tgaccaggtt aaacaacgca cgtccgttag
     8401 agagacggtc aagcgtggcg gcctgccggg cggcaacggt aggtgaggtt acgctggggc
     8461 gcagggcgac aagaaacttc agccgttgtg tcacagggat catcgacgcc gccaccagcc
     8521 acgcatcttc acacgatcgc cccgtcggga tcagcacacc ggtatagccc agacgatccg
     8581 ccgcttgcgc aatttgttgc agataaccgt gatcaaccgg acgtgaacct tcttccgttc
     8641 ccagataatg cccgtcaccg tgggtcggta aaaaccagaa catattcaga ctcataattg
     8701 ttttccttcc agttgagtgg gttgccagat gcgctggcga atatcgactt ttttcggcac
     8761 cagacggttt tcataaaaca gatctgccgt ttgctgctgt aaggcggcaa cttcagcgct
     8821 caacggtttg atggtggtgg gaggacgatg atccagatac gaggcaatca ccggggccgg
     8881 taagcccatc gtttttgcca gtaaagcgat gctttgttcg cgctggctgc gggttaacgc
     8941 atcggcctca ctaaaggttg tcagcacacc ctgaataaaa gcgccgtttt tttctgcata
     9001 cggacgagct gccagataaa acgatccagt ttgattgaga tcggtgccgt ctttcagcac
     9061 ccgcacgccg ccctgtaata atgcagcgga gtagtaggga tcccagatag cccaggcgtc
     9121 gacgttacct tgctggaacg cggcgcgggc atcggcaggc gtcaggtaag tgggctgaat
     9181 atccgtgaat ttaagcccgg cctggcgaag ggctcgcagt aaaagattgt gcgaactgga
     9241 acctttctga aaggcaactt tgtgaccttt aagatcggct acggttttga tcgggctgtt
     9301 ttctgccacc agaatcactt cggctttggg cttcggtggc tcgacgccca cgtataccaa
     9361 atcagccccg gcagcctggg caaagattgg cggaatatct ccggtactgc cgagatcgat
     9421 actgccaacg tttaacgcct ccagcatctg tgggccagcg gggaactcca cccaggagat
     9481 ttttgtttgc ggatagcgtt tttccagtaa ctggtggctt tttgccagca ccataccaat
     9541 actgcctttt tgatagccta tacgtaacgc ctctggcgaa gactccgcgg caacagcaag
     9601 tgtagagacg ctaagtaaac ccgccagcgc cagtttaatg atcttacgca tgggcattac
     9661 ctcgcagaga cagaaggtca ggaacctgaa catcgcggcg gtgcaacgcc tgccagaaag
     9721 tttctagcgc ggtatcaaga cgagtttgca gatttggcgt gaacttgggt ttgtgttggt
     9781 aatcaatgac ttgcgagtca tcggcaaaca cgccgtgcag gatctcctgg gctttcagcg
     9841 tacttaaaac tggcttaagg gcataatcga ccgccagcag atgggccacg gtgccgcctg
     9901 tcgccagcgg cagcaccact ttgccttgca acgcacgttc tggcagcagg tcgagcaggg
     9961 ttttcagtgc accggaatag gcggctttat acacaggcgt ggcgacaatc agcccatcgg
    10021 cctgttgcag ctgttcggtg aaggtcttga gtgccggact atcgaaacga gcataaagca
    10081 gatcttccgg gacgaagttt tgcaaattcc agtgataaac ctctacatcc aggccattta
    10141 gtttttcccg cacatattcc agcaaggagc tggagcgaga aggaaagcga ggactacccg
    10201 ccagggtgat gacacgcata ctctctcctt ataaccaatt gttcttgttt tgttaacatt
    10261 gataacaatt cggtcagtct gacagagggc gtccgcgagt cgaaatgatt tatctgagga
    10321 agaaaagcca aaaaaagcat aaaaaaagcg caataagaaa acgtttgcta tttattgccg
    10381 caggtcaatt cccttttggt ccgaactcgc acataatacg cccccggttt gcacaccggg
    10441 aatccaggag agttcatgta ctaccccttc gttcgtaaag cccttttcca gctcgatcca
    10501 gagcgcgctc atgagtttac ttttcagcaa ttacgccgta ttacaggaac gccgtttgaa
    10561 gcactggtgc ggcagaaagt gcctgcgaaa cctgttaact gcatgggcct gacgtttaaa
    10621 aatccgcttg gtctggcagc cggtcttgat aaagacgggg agtgcattga cgcgttaggc
    10681 gcaatgggat ttggatcgat cgagatcggt accgtcacgc cacgtcccca gccaggtaat
    10741 gacaagccgc gtctctttcg tctggtagat gccgaaggtt tgatcaaccg tatgggcttt
    10801 aataatcttg gcgttgataa cctcgtagag aacgtaaaaa aagcccatta tgacggcgtt
    10861 ctgggtatta acatcggcaa aaataaagat acgccggtgg agcagggtaa agatgactat
    10921 ctgatttgta tggaaaaaat ctatgcctat gcgggatata tcgccatcaa tatttcatcg
    10981 ccaaataccc caggattacg cacactgcaa tatggtgaag cgctggatga tctcttaact
    11041 gcgattaaaa ataaacaaaa tgatttgcaa gtgatgcacc ataaatatgt gccgatcgca
    11101 gtgaagatcg cgccggatct ttctgaagaa gaattgatcc aggttgccga tagtttagtt
    11161 cgccataata ttgatggcgt tattgcaacc aataccacac tcgatcgttc tctggttcag
    11221 ggaatgaaaa attgtgatca aaccggtggc ttaagtggtc gtccgcttca gttaaaaagc
    11281 accgaaatta ttcgccgctt gtcacaggaa ttaaacggac gcttgccgat tattggtgtt
    11341 ggcggcattg actcggttat cgctgcgcgt gaaaagattg ctgcgggtgc ctcactggtg
    11401 caaatttatt ctggttttat ttttaaaggt ccgccgctga ttaaagaaat cgttacccat
    11461 atctaattat ttcttcaaat tcctctttat aagataatca gggctttatt ttcagccctg
    11521 gttgttttat attcatcgct gttgcttatt tagacatttt gtacttttat tgttgaggtt
    11581 attaagcgaa gcgacaatgg attgtgtcgc tgcggtttta taaggttggg gagtggcaga
    11641 tgcgaattaa accagacgat aactggcgtt ggtattacga tgaagagcac gatcgaatga
    11701 tgctcgattt agccaatggt atgctatttc gctcacgttt tgcgcgcaaa atgttgactc
    11761 cagatgcctt ttctcccgca ggtttttgcg ttgatgacgc cgcgctctat ttctcttttg
    11821 aagaaaagtg ccgcgatttt aatttatcta aagaacaaaa agccgaactg gtgctgaatg
    11881 cactggtagc aattcgttat ctcaaaccgc aaatgccgaa aagctggcat tttgtatctc
    11941 atggtgaaat gtggatgccc atgccgggcg atgcggccta cgtttggttg agcgacactc
    12001 atgagcaagt gaatttactg gtcgttgaat ctggcgagaa tgccgcgcta tgtctactgg
    12061 cacaaccctg cgttgttatt gcgggtcgcg cgatgcagtt gggcgacgcc attaaaatca
    12121 tgaacgacag gctgaaaccg caggttaatg ttgacagctt cagcctcgaa caggcagtct
    12181 aacgcgccgg cttaagcgca gtcttcggta cacaactaca gcaaagaata gtgccatcat
    12241 cgcctattgc tgattttttt agcggcgtga cttcgccttc taaaagctga acacggcaac
    12301 ttccacaaat gcccgcgcgg caagaataag ggatacgaat tccctgattt tctaattgct
    12361 ccagcagcac ctgttggtta tttccacgaa atgcctgtcc ctgccagtca atatctacgt
    12421 ttgcgtccgg ttgttgcgtg atgttggcag tatcatcagc ggcacctgcg ccgtaaattt
    12481 tagccggagc cgttgccaga atttccacct cgtcgccaac gcgaatcacg ccgctattac
    12541 gggcaattaa attctgacca aaatcgacat cgccgttatc ctgggcagta cggaaagatt
    12601 gcagtgtttt taatggttcg cctgccggat gtttttgccc tttttctggg ctgacggtgg
    12661 tgaaaataca gcggctacaa ggcttaacca catcaaacac gacatcacca atgcgaacca
    12721 ctttccagct atcttcttcc caggccgatg caccggaaac caccagattg gggcggaatt
    12781 gctccatttt tacactggcc ggacaacgtt gttggagatc acgtaacgag gcctcgttag
    12841 caagaaggta aggatagcca tcagcaaatg acaggggtac agtgttgtgg cgtttcacgc
    12901 gccgggtcat ttgtggcccc acccagcgta attgcacttc gcgggagaaa aatccactta
    12961 gccatttgtt gatcgcgtct ggcgcaattc gcgcggtaaa atgtgtgccc caaacttcgg
    13021 ttggtgcgtc ttgtgtggcg aaatcagcaa aacgaacata tgcactactg ccatctggtg
    13081 cggtgagatg caagccatca tgcacgggtg aaggagtaaa ccgtaccatt agcggaaact
    13141 ggcgagcggt aataaacgta ccgtcaggtt ccgtgatcat aaagatgcga tcgaaggcca
    13201 gaccactgac atctgccagg gtatgtgtaa gaccaatgcc gcgcatcgat ttaacaggat
    13261 gaataaaaag ccgggttaat gtcgccacgg tgcggtcctc aaatgaaaat aagccctcaa
    13321 ctttatgaca tgacgcgctt attagctata atgcgcaaca attttcttag agtaaaagtg
    13381 acgatatgaa ttctctgttt gccagtacgg cccgtgggct ggaagagctg ttaaaaactg
    13441 aactggaaaa cctgggggcc gttgaatgcc aggtggttca gggtggggtc catttcaagg
    13501 gcgacacacg gcttgtttac cagagcctga tgtggagccg cctggcctcg cgtattatgt
    13561 tgccgctggg cgagtgtaag gtttacagcg atttagacct ctatctcggt gttcaggcga
    13621 tcaactggac agagatgttt aatcctggcg cgaccttcgc tgtccacttc agtggtttga
    13681 atgacaccat acgcaacagt cagtacggtg cgatgaaagt gaaagacgcg atcgtcgatg
    13741 ctttcacgcg gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg
    13801 ttaacgtctg gctgcataaa gaaaccgcca gtatcgccct tgatctcagt ggtgatggtt
    13861 tacatctgcg tggctatcgc gatcgtgctg gcattgcgcc gatcaaagaa accctggcag
    13921 ccgcgattgt gatgcgatcc ggctggcagc caggaacacc actgctcgat ccaatgtgtg
    13981 gttccggtac gttgctgatt gaagcagcga tgctggcgac cgaccgcgca ccgggcttgc
    14041 accgtggccg ttggggcttt agcggctggg cgcagcatga tgaagctatc tggcaggaag
    14101 tgaaagcgga agcgcaaact cgtgcccgta aaggcctggc ggagtatagt tcccatttct
    14161 acggttcgga cagtgacgca cgggtgattc aacgtgcgcg cactaacgcc cgtcttgcgg
    14221 ggattggtga actgatcacc tttgaggtga aagatgtcgc gcaactgacc aatccgctgc
    14281 cgaaagggcc gtacggtaca gtgttgagca acccgccata cggtgaacgt ctggacagcg
    14341 aaccggcgct gattgcgctg catagtctgc tgggccgaat catgaaaaac cagttcggtg
    14401 gctggaatct ctctttgttt agtgcctcgc cggatctgct aagctgcctg cagctgcgtg
    14461 cagacaaaca gtacaaggcg aaaaacggcc cgctggactg cgtacagaaa aactaccatg
    14521 ttgccgaaag cacaccagac agcaaaccgg taatggcagc ggaagactac gccaatcgtc
    14581 tgcgtaagaa cctcaaaaaa ttcgagaagt gggctcgcca ggaaggaatt gaatgttacc
    14641 gcctgtatga cgccgatctg ccggaatata acgttgccgt tgaccgttat gccgactggg
    14701 tggtggtgca ggagtatgcg ccgccaaaaa ctattgatgc gcacaaagcg cgtcagcgtc
    14761 tgttcgatat tatcgctgca accatttcgg tactggggat tgcgccaaac aaactggtgc
    14821 tgaaaacccg tgaacgccag aagggcaaaa accagtacca gaaactgggc gagaagggcg
    14881 agtttctgga agttaccgaa tataacgctc acttgtgggt gaacctgacg gattacctcg
    14941 ataccggtct gttcctcgat caccgtatcg cccgtcgtat gcttggtcag atgagcaaag
    15001 gcaaagattt cctcaacctg ttctcttata ccggcagcgc caccgtgcat gcgggattag
    15061 gcggtgcacg cagcaccacc accgtggata tgtcgcgtac ttatctggag tgggcagaac
    15121 gcaacctgcg tctgaatggc ttaactggcc gtgcgcatcg cctgattcag gccgattgcc
    15181 tggcgtggct gcgtgaggca aatgaacagt tcgatctgat ctttatcgat ccgccaacct
    15241 tttctaactc aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat ctggcgctga
    15301 tgaaagattt gaaacgtctg ctgcgtgcag gtgggacgat catgttctcg aacaacaaac
    15361 gcggtttccg tatggatctc gacggtctgg cgaaactggg actgaaagca caagaaatta
    15421 cgcaaaaaac gctctcccag gatttcgccc gtaaccgcca gatccacaac tgctggctga
    15481 ttaccgcagc ctgaaaggaa tagtaatgtc attaatcagt atgcatggcg catggctgtc
    15541 gttcagcgac gcgccgcttc tcgataacgc agaactgcat atcgaagata acgaacgtgt
    15601 ttgtctggtg gggcgtaacg gcgcaggcaa atcgacgtta atgaagatcc tcaaccgtga
    15661 acaggggctg gatgacggtc gcattattta tgagcaagat ttgattgtag ctcgtctgca
    15721 acaggatccg ccgcgtaacg ttgagggtag cgtttatgat ttcgttgccg aaggcattga
    15781 agaacaagcg gaatacctga aacgctatca cgatatttcg cgcctggtga tgaacgaccc
    15841 gagcgagaaa aatctcaacg aactggcgaa ggttcaggaa cagctggatc accacaacct
    15901 gtggcagctg gaaaaccgca tcaacgaagt gctggcgcaa ctgggattag atcctaatgt
    15961 tgcgctgtcg tcgctttccg gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt
    16021 gagcaatccg cgcgtattgc tgctcgacga accgactaac cacctggata ttgaaaccat
    16081 cgactggctg gaagggttcc tcaaaacctt caatgggaca attattttca tttcccacga
    16141 ccgttcgttt atccgcaata tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt
    16201 gacctatcca gggaattacg accagtacct gctggaaaaa gaagaagccc tgcgcgtgga
    16261 agaattacaa aatgccgagt tcgatcgcaa actggcgcag gaagaggtgt ggatccgcca
    16321 ggggatcaaa gcacgtcgta cccgtaatga aggccgcgta cgcgccctga aagcaatgcg
    16381 tcgtgagcgt ggtgaacgtc gcgaagtaat gggtaccgca aagatgcagg tggaagaagc
    16441 cagccgctcc ggtaagatcg ttttcgaaat ggaggacgtt tgctaccagg ttgacggtaa
    16501 gcaactggtg aaagattttt ctgcccaggt tctacgcggc gacaaaattg ccctgattgg
    16561 tccgaatggg tgcggcaaaa ccacgctgct gaaactaatg ctcggtcagc ttcaagcaga
    16621 cagcgggcgt attcacgttg gcaccaaact ggaagtggct tatttcgatc agcaccgcgc
    16681 ggaactggat cccgataaaa cggtgatgga taaccttgcc gaaggtaagc aagaggtgat
    16741 ggttaacggc aagccacgcc acgtattggg ctatttgcag gacttcctgt tccatccaaa
    16801 acgggcgatg acgccggtac gtgcgctttc tggcggtgag cgtaaccgct tgttgttggc
    16861 gcgtttgttc ctcaaaccaa gcaacttatt gattcttgac gaaccgacca acgatcttga
    16921 tgtcgaaacg ctggaactgc tggaagaact gatcgacagc tatcagggca cggtattgct
    16981 ggtgagccac gatcgtcagt ttgtcgataa caccgttaca gaatgctgga tcttcgaagg
    17041 cggcggtaaa attggtcgtt atgtcggcgg ttatcatgat gcccgtggtc agcaagaaca
    17101 gtatgtggcg ctcaaacagc ctgcggtgaa aaaaagcgaa gaagccgccg cgccaaaagc
    17161 agaaactgta aaacgcagca gtagcaaact aagctataaa ttgcagcgtg aactggagct
    17221 gctaccgcaa ttgcttgaag atctggaggc gaagctggaa gctttacaga cgcaagtggc
    17281 ggatgcttcc ttcttcagtc agccgcatga gcagacgcaa aaagtgctcg ccgatatggc
    17341 tgctgcagag caggagctgg agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa
    17401 aaatggtggc tgatcgcaaa atagtcgatt taaacagaga ctgctgacaa cggtaaaagt
    17461 gcctgatgta ctgggtttat caggcatgaa aaccaacact ttgttagtca tctgcaaaat
    17521 agcgcatcat taaggagtac caatgtgcga acatcatcat gccgcgaagc acatcctgtg
    17581 ctcgcagtgt gacatgctgg tggcgttacc gcgccttgag catggtcaga aagcggcatg
    17641 tccccggtgt ggcacaacgt taaccgtggc gtgggatgcc cctcggcagc gtcccaccgc
    17701 ctatgcgttg gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat
    17761 gaacgttgca ggagttacca gtgaaattac attgctggaa attcccggcg tgcttttttc
    17821 tgaggactac gccagcctcg gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt
    17881 ttgtctgata accattctgt tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga
    17941 gcaactggca cgggtgcttt ttcaactcaa aacctgggga atggcggaga ttttcctcgc
    18001 gggtgtgctg gtcagtttcg ttaaactgat ggcttacggc agcattgggg taggcagcag
    18061 ctttctcccc tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg
    18121 tcgctggtta tgggacgaca tcgccccgat gccagaactg cgccagccgc taaaaccagg
    18181 tgtcacgggg atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc
    18241 tgatgaaccc gtgtgcccgc gttgtagtac caaagggtac gttcggcgta gaaacagcct
    18301 gcagtggaca ctcgcgctgc ttgtaacgtc catcatgctg tatcttccgg ctaatatttt
    18361 gcccatcatg gtgacggatt tattaggctc gaagatgccg tcgacgattc tcgctggggt
    18421 cattctgtta tggagcgaag gctcttatcc cgtcgctgcg gtgatttttt tggccagtat
    18481 tatggtgcca acgttaaaga tgatcgccat tgcgtggctg tgttgggatg ccaaaggaca
    18541 cggcaagcgc gacagtgaaa gaatgcattt gatttatgaa gttgttgagt ttgtaggccg
    18601 ctggtcgatg attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg
    18661 tttaatgagt atttatccgg caatgggtgc attaatgttt gctttagtcg tcataatgac
    18721 aatgttttct gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga
    18781 gcatgaggag tcctgacagt atggaatcta ataatgggga agccaaaatc cagaaagtga
    18841 agaactggtc tcccgtgtgg atatttccta tcgtcacggc gctcattggg gcctgggttc
    18901 ttttttatca ttacagccat cagggaccgg aagtgaccct gatcaccgcg aatgcggaag
    18961 gaattgaagg tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa
    19021 gcgccacact ggctgatgat ttgacgcacg ttgaaatcaa agcgcggctg aattccggta
    19081 tggaaaaatt gctgcataaa gataccgtct tttgggtggt gaaaccgcag attggtcgag
    19141 aagggattag cggcctggga acgctactgt ctggggttta tatcgaactg cagccaggcg
    19201 cgaaaggcag caaaatggat aaatacgatt tgttggactc gccaccgttg gccccgcctg
    19261 atgcgaaagg tatccgtgtg cttctcgata gcaaaaaagc cgggcagctc tcgccaggag
    19321 atccggtgct gttccgtggc tatcgggtag gttcggttga aaccagcact ttcgatacgc
    19381 aaaaacgcaa tatcagctat caactgttca tcaatgcacc ttatgaccga ctggtgacca
    19441 gcaatgttcg tttctggaaa gatagtggca ttgcggttga tctgacgtca gcgggaatgc
    19501 gtgtggagat gggctcattg acaacgctgc ttagtggcgg tgtcagcttt gatgtgccgg
    19561 aaggtctgga tttagggcag ccagtggcac cgaaaacagc tttcgttttg tatgatgatc
    19621 agaagagcat tcaggattcg ttgtacaccg atcacattga ttatctgatg ttctttaaag
    19681 attcggtacg cggtctgcaa ccgggagctc cggtagaatt ccggggtatt cgcctgggta
    19741 ccgtaagcaa agtgccattc tttgcgccga atatgcgtca gacatttaac gatgattacc
    19801 gtattccggt actgattcgt atcgagccag agcggctgaa aatgcagctt ggcgaaaatg
    19861 cggatgttgt tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa
    19921 ccggaaacct ggtcactggc gcactgtatg ttgatctcga tttctatcca aatacgcctg
    19981 caataaccgg tattcgtgaa tttaatggtt atcagattat cccgaccgtc agcggcggcc
    20041 tggcgcaaat ccagcaacga ctgatggaag cgttggataa gatcaacaaa ctgccactga
    20101 atccgatgat tgaacaggca accagtacgc tttctgaaag tcagcgcaca atgaaaaacc
    20161 tgcaaacgac gctggatagc atgaacaaga tccttgccag ccagtcgatg cagcagttac
    20221 cgacggatat gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg
    20281 gttccgcagc ctacaacaag atggtggcgg atatgcagcg ccttgatcag gtgttgcgag
    20341 aactgcaacc ggtgctgaaa acgctcaacg agaagagtaa cgcgctggta tttgaagcga
    20401 aggacaaaaa agatccagag ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat
    20461 tgcggcactg tggctggccg gatgcagctc cggcgaaatt aataaaaact attaccagtt
    20521 acctgtggtg catagcggta cacaaagcac cgccagccag ggtaatcgcc tgttatgggt
    20581 tgagcaggtc gctgttcctg actatctggc ggggaatggt gtggtttatc aaaccagtga
    20641 tgtgaagtat gtgattgcca acaacaacct gtgggccagc ccgttggatc aacagttgcg
    20701 caacaccctg gttgccaacc tgagtacgca actacccggc tgggtggttg cctcccagcc
    20761 tctgggaagc gcccaggaca cgctcaatgt taccgtcacg gagtttaacg gtcgctatga
    20821 tggcaaggtc attgtcagtg gtgagtggct gttgaaccac cagggacaac tgatcaaacg
    20881 tccgttccgt ctggaaggag tgcaaactca ggatggttac gatgagatgg ttaaagtgct
    20941 ggccagtgtc tggagtcagg aagccgcttc tattgcgcaa gagataaagc gtctaccgta
    21001 atcataaaga tttgtaaata taaccgtctc cggtatgttg cctgaggcgg tttttttgtt
    21061 tctaacgtgc ggaaaaagtt gttcctcttc acattttttg tacaaccggc atgcccgtgt
    21121 agctcacaaa tatgacagtg gcgtgaattt tgcgcattga cggcagttat gattcgcggt
    21181 attgcttaac tgtgattgca tatttagtaa tcactgtttt cttttccacc agaaaccagt
    21241 atgagggaaa cgaggcatga agagacaaaa acgagatcgc ctggaacggg cacatcaacg
    21301 tggttatcag gccggcatcg ccggacgctc aaaagaaatg tgtccctatc agacgctgaa
    21361 tcaaaggtca caatggctgg gaggctggcg agaagccatg gcggacaggg tagtcatggc
    21421 ctgattctgt ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct ttatatactc
    21481 tgtctgatta aaatcagaat gcagacgtat cctggaacag acctactttc aggtcgttgg
    21541 cggtatagat caaacgacca tcaaccagca cttcgccatc agccaggccc ataatcagac
    21601 gacggttaac tatgcgttta aagtgaatac gataggtcac ttttttcgcc gtcggcagca
    21661 cctggcctgt gaatttcact tcacctacgc ccagcgcgcg acctttacct tcgccgccca
    21721 gccagccgag gtagaaccct accagctgcc acattgcgtc caggcccagg catcccggca
    21781 taaccggatc gccgataaag tggcatccga agaaccacag atccggattg atatccagct
    21841 ctgcttcaac ataccctttg tcgaagttac cacccgtttc ggtcattttg accacacggt
    21901 ccatcatcag catgtttggt gctggcaatt gcgggccttt agcgccaaac agttcaccgc
    21961 gaccagaggc aagaaggtct tcttttgtat aggattcgcg tttatctacc atgttctctg
    22021 taagccttat tttattgaag cacgcaggat agctaacacg tgtacgctga acaagtccga
    22081 tcagttcgga ataaaccagt tcagccaacg taatggccat ggaaaacggt gacgtccttc
    22141 ctgttgcgat gcttgtgcga tacgttcctg gatggtttgc atcagcgtcg tttggccttc
    22201 gccatcccac accagattta ataataacgg cagtgcgtca gtcacatcgt ctactgccca
    22261 gatggtgaat ttgtcttctt ctaccgcttt caccagttca ctgtgaagac ttaaatggcg
    22321 aacgttagct gtggggataa tgacaccttg tttccccgtt aactcacgtt gctggcaaat
    22381 agcaaagaag ccttcgattt tctcatttaa accaccgact ggctgggcgc gaccgaactg
    22441 atcgactgaa cctgtgatag cgatactctg attcaccggc acatcggcga gggcgcttat
    22501 cagggcgcag agttcagcca tcgaggcact atcgccatcc acttcactgt atgactgctc
    22561 aaatgtcagc gatgctgaga aggggatctg ttgctcaagc tgtagttccg acatcaggaa
    22621 cgcttgcatg atcatcatcc ctttcgcatg gatattgccg ccaagctccg ctttgcgttc
    22681 gatgtcggtg aattcaccat cgccaatatg tacaacgcag ctaatgcgag aaggttcgcc
    22741 aaaagcgcgt ggatgacccg gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg
    22801 ttcgccttcg gtttcaatca ggatttgctc ctgaaggatc tcatcctgca tgcgttcagc
    22861 gaggaaacct tcgcgccatt cacgctgctg tagcattaag tttagctgct cgccggagaa
    22921 ggtgtcgcca tcgcacaggg aggcgacctc tttacactgg cggaggatcc actgcgggct
    22981 aagcggaagt gtttcttgtt caccagtgta gcgtgctgct tcgcggataa gcaccgacca
    23041 ggcatccgct cccggtgctg gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca
    23101 ccactggctt actgactccg catcgacaat ctgcagagta tcttcaaatt cgctataaat
    23161 agcctgctct gaaagctctg gctccatctc ctggaaatca gccaatgatt cgcgttcgcc
    23221 taccagaatg actttcagct tcaatggcat cgaaggcaca gagacgggga gagggcgcga
    23281 ctcatcaaac gcaacccagt caaaacgctc gcggttaacg atatttttca gccgcatcca
    23341 cagcagaggt tgcgccagca gtgtacgcaa agagatgatg agaataccgc catttgcctg
    23401 atgcaccaga ccaggctgca gggtaatatc gccattaaac tgacgcaggc agccaaagag
    23461 ttgctctgct tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc
    23521 tgcactcact gcgtgacgta agcggatggt gtggccggaa acctcatagt gaccgcccac
    23581 cagttggcct gcatcgcttt gtagcgtacg cgcggcatca gcaataagat tcagatactc
    23641 agactcttcc ggggccttcg ccagcataaa agaggaggat gctcgcgtat gcagcaactg
    23701 ctccagcgca aattgcagtc gcggttgagt atcactgaat aaaggatcgt tttcgtcaat
    23761 caaatgtggc tgagcaaata tttcctgata gctatcggta tcaggaacca ggtcacgcca
    23821 tgcaagtttc gtaatggtca aagttgatgt tttttagtct gttgtcaaag ccgcgattat
    23881 accgtaaccg gcactgcagc acacgtagaa agcaacgaca atactcctgg catgggcgtt
    23941 aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag
    24001 agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg
    24061 gttggaaatg gaagtatctg gtgaagaagc accgcgaagg ggagttaatc acccgttaca
    24121 tagaagccag tgccgcccag gaagccgtcg atgagttgct ctcgctggaa aatgaacccg
    24181 tgctggtaaa tggttggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc
    24241 agaccattcg ggccagacgg aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa
    24301 aatcgatcga tctggaattt atcgtctggc aacgtctggc cggccttgcg cagcgtcgcg
    24361 gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat
    24421 acgcgaataa aatgtcttct ttgaagcagg atctgcaggc attgctgggt aaggaataac
    24481 tgtcaaaagt cagttcaatt tactaaaggc gaaaaaaaac cccgcagctg cggggttttt
    24541 ctaccagacg agaacttaag cctgcggctg agttacaacg tctttgatgc ctttaacttc
    24601 gatctctacg cgacgatccg gagccaggca atcgatcagt gcagcacgct gtttcacgtt
    24661 gtcacaggtg ttgccagtaa ccgggttgga ttcgcccata ccacgtgcgg agattttgtc
    24721 tgccgggata cctttagaga tcaggtaatc aacaacggac tgagcacgac gctcggacag
    24781 agcctggtta taagcgtcag aaccgatgcg gtcagtgtaa cccagaacaa ctacggaacc
    24841 gtctttcgga tccaggttgc tcagctggct gtacagctga tccagagcag cctgaccttc
    24901 cggtttcagg gttgctttgt tgaaggtgaa cagaacgtca gacttcagag tgaagtgctt
    24961 ggtctgtact tccggtgccg gagctggagc cggagcaact actggagctg cttcgccctg
    25021 accgaaacgg taggaaacac ccaggctcag catgccgttg tccggacgag tgccgatggt
    25081 gtgtgcgtcc ccgatgttgt tggtccactg gtattccaga cgggtagcga tttcaggagt
    25141 gatcgcatac tcaacaccgc cagcgaagac cggagaaacg ccggtgtcgt ggtctttaaa
    25201 ggatgcgcca ccaggtacgt tagccttggt gtctgcacgc cataccatac cacccagacg
    25261 agtgtagatg tccagatcgt cagtgattgg gtaacccagt ttagcggtca gctgaacgcc
    25321 ctgagctttg tatgcgccgt tgatgttgtc gcctttgtac ggcatacgac ctaaccagtc
    25381 gtaacccatt tcaaagccaa catacgggtt aacctggtaa ccaccaaaag cacctgcacc
    25441 cagttggttt tcgtgggtcg gaccattgtt aggaataaaa ccagtgtcat ggtactggga
    25501 ccagcccagt ttagcaccag tgtaccaggt gttatctttc ggagcggcct gcgctacggt
    25561 agcgaaacca gccagtgcca ctgcaatcgc gatagctgtc tttttcattt tttgcgcctc
    25621 gttatcatcc aaaatacgcc atgaatatct ccaacgagat aacacggtta aatccttcac
    25681 cggggatctg ctcaatatta actctaccga tatcttcggc ttatgccgag cacccctggc
    25741 gatgtaaagt ctacaacgta gttgaaaact tacaagtgtg aactccgtca ggcatatgaa
    25801 aaaaaagtct tgtataaggt acgtttaatc ttttttgtca gcgacaattt acagaagaaa
    25861 atcgcggaaa ccgcttcaga caagcctccg caaggaaaat tagtcacgac tgaaagcatt
    25921 ggctgggcga caaaaaaagt tccaggatta atcctaaatt tacttaatga tacaaattag
    25981 agtgaatttt tagcccggaa agttgtctcg ttgcgtgaga ggatgcgctt accggacgca
    26041 taataaaccc catagcgtta ccttcatttg ccgcatcaac aagttcagca tgctcttctg
    26101 cagtcaaatc atctgccaac caaccgatca ccacgctgta attgcccgtg cgtaaagcgc
    26161 gaaccattga ctccacggtg tggcaagggg agagctggct aatctgcatt actttcgtta
    26221 agggtagccc agatgcctga acccattcgc gactcagttt ttgttgcggt gttaaccaga
    26281 gttgccagcg cgattgctga ccgagttgct gtaacaatgg caacagtaga agttgcgtca
    26341 tcatgggctg atcttcgcga tagacaactt cactgataag cccggctgta gtgttttccg
    26401 tagagacacg cgcaatttta cttgctgtgg atgagaacga cgaatcacga tgtgcatagc
    26461 ctgaagtgta cataatcaat ccagcccctg tgagttactg tatggatgta cagtacatcc
    26521 agtgaagaca aagatcaact ctattttcgg aaagagcctc gcaaattttg tcgttggtga
    26581 cgggaaaaca taaattaatc ttgcccctta agaataagtt gcctattttc gtagttaacg
    26641 gatccgttaa tgtgaatcat tcttttatgt tatgatttta aaaggaattt tatgaaaagc
    26701 ctctcctata agcggatcta taaatcacaa gaatacctgg caacgttggg cacaattgaa
    26761 taccgatcat tgtttggtag ttacagcctg accgttgacg acacggtgtt tgcgatggtt
    26821 tctgatggtg agttgtatct tcgggcttgt gagcaaagtg cacagtactg tgtaaaacat
    26881 ccgcctgtct ggctgacata taaaaagtgt ggccgatccg ttaccctcaa ttactatcga
    26941 gttgatgaaa gtctatggcg aaatcaactg aagctggtgc gtctgtcgaa atattctctc
    27001 gatgcagcgc tgaaagagaa aagcacgcgc aatacccggg aaagactgaa agatttgccc
    27061 aatatgtctt ttcatctgga agcgattctc ggggaggtgg ggattaagga tgtacgggcg
    27121 ttacgtatac ttggggcaaa aatgtgttgg ttgcgactgc ggcagcaaaa ctgtctggtg
    27181 acagaaaaga ttctgtttat gcttgaaggg gccattatcg gcattcatga agctgcgctc
    27241 ccggtggcac gccgccagga gcttgcagaa tgggctgact ctcttacgcc aaaacaggag
    27301 tttcctgcgg aacttgagta atctggcgtt gcagacgacc aatctctggc agtaatgcaa
    27361 tcaataaccc aacttgttgt acgaccagag gttctttgct gtctgcccgt ggttcaagtt
    27421 gctgtatccg ctgtttcagg ccagctaatg cctgattgac gcgttcttca tcagcaggtt
    27481 gatgatgtag cgcgtcatca acatagcaaa ctgcgtcatc aagaaacgcc agaatttcag
    27541 gattagttaa ctgctcccgg tgagcaccga gggctgagat atagctggta aacgtatggt
    27601 taaggcacag caaccgaaac gcggcttcgc gaatttgcgg ggtaacgttc ggttcgctgg
    27661 acatatttga taccaccgac gccagttcag catcacggtt gtgtgcatcg cggcgggcaa
    27721 tacgatacgc cagacggtta tcacgcccct gatggtattg ctccagtatg gcatcgagat
    27781 accgacagtt ggcctctgtg gcgcgttcga gcattcgcgg cagattgcga aacttccagt
    27841 caggccagat gtagctcact gcagcccacg caatggcaca accaatcagc gtatcgatta
    27901 cgcgaggtaa cgccacttca aaaccttcac ccagcaagtt gaaacacagt agcaccaaaa
    27961 gtgtgatgaa catcgttgca tgggcgtatt gcacgttacg gaaggcaaaa aagagcacgc
    28021 cggtaataac cagcagcacc agctgccctt ccagtgatgg cacaaaccac agcacaggaa
    28081 tgccaattgc gatacctacc agcgtaccaa taatccttaa cttcaggcgg tggcgcgtgg
    28141 cgttatagtt tggctggcag acaaacaaac ttgtcagcaa gatccaatac ccgtgatgca
    28201 ttccggttat ctgaatgatg gcgtagccga agcacaacac cagcgacatt cttactgcat
    28261 gacggaagag ggcggattcc ggcgtgaagt gacggctaag acgcagccag atatcactca
    28321 atccgtgcgg gctgtcatca gcgagctcat tttcgtcatt attatggggc agtgcctggg
    28381 cctgttctga ttcaattgtt gccagttggg catcaatggc acgtaaattg ttcagtaaaa
    28441 atcccagtgt tttgagtaaa tcagcaggag cgtcgttatc gcgcatccgc tccagcgcag
    28501 catcaatatg cgtaaaagcg cgctcaaaat gcggatcatg ttgataaggc tgacgcagca
    28561 aaatacagcg tgacagttgc tggcacgcct ggccctgcat cgacatcagc cgctgaaaac
    28621 ggaacagcac gtcgctgtgg cgaaaatgtt cacgcaatgt ttgatattga atatgagaag
    28681 agctggcacg ctcgtgaata tcctgcgcga caaagtaata atgcagtgtg cgacgcgttc
    28741 cccgttgacc acgatcgcca cgtaagcggg tcagcagcga gagtttcgtc tgattcaatg
    28801 tcgccatcag ctgaccgttg gcgagagcca aatcgtacag cggtgcctgg ctttcatctt
    28861 caatatcagg atcaaacatg cgcgacttga gctcaagata acgcgccagt tgttcatagc
    28921 aacgcgccag gttgtcctgc agcgggcgga ccgggaacag tagatgacca ataagtgtca
    28981 ggacgttgta ccagacggca ccggccagaa gatacatcgg ctgctgatac cagtgctcat
    29041 acagtgatgt tcccaacata gtgtaaatgg cgatcagcaa tgcaccgaag gcaattgttg
    29101 catagcgttg acccagaccg ccgagcaaaa tgaagccgct ggtagagagc gttaagccaa
    29161 tcgcaaatag ccagggccag ggaaacagca attctactga ggccgaggcg ataaaaaagc
    29221 agaacagcgt aatgatgagg ttacgcaaac gtcccgccag tcggtcatcg agatcggtca
    29281 gcgccgctgc caccatccca agcgtcagcg gaatcgtcag ttttacatca cccagccacc
    29341 acggaaacgc tgtggttcca caaagcgcaa taaaaatacg cgcgtaatac agccaggcgc
    29401 tgttccaggt atagcgtttg agcaaaggac ttagcataaa ggccatatta gtggattatt
    29461 ggaaacgacg acgtgcattg gcttcgcgcg cagcttgcgc tgtttctacc gataccacgc
    29521 gacgaccgac cggccatagt gcaatagcgg caattttaaa gttcgcaata ccgacaggaa
    29581 tgccaatgat ggaaatacat tgtgcgatgc ccgttgcaat gtgcatcagg cataaccacc
    29641 agccaaagaa aatcagccag aaaatattca ataccgtacc gccagtattt agcagcacat
    29701 ttttgccagc cgggttcagt tcatcgacat gaatagcttc attgccataa ggcaccagag
    29761 acagtttggt gatctcccag caggatcgtg tcagtggtaa ggtaaaaatc agcacaatac
    29821 tgaccagagt cgccaacagc cagcccagag tggtggcaaa tccgccaagc acaaagttca
    29881 gaatgttcaa aacggtacgc ataaaacctc gctttactgt ggttttcagt aatgggcggc
    29941 aattgtaacg tttttttggg ctggagcacg ttttctctga cggttacact gataagaaat
    30001 aatttcgtgt ggatctacag agtcatggaa ctgaaagcga caacgcttgg aaaacgtctg
    30061 gcacagcacc cttacgatcg ggcggtgatc ctcaatgccg ggattaaagt ctccggcgat
    30121 cgccacgaat accttattcc tttcaatcaa ttactggcga ttcactgtaa gcgcggtctg
    30181 gtatggggcg aactggagtt tgtactgccg gacgaaaaag tggtgcgtct gcacggcacc
    30241 gaatggggcg agacgcagcg tttttaccat catcttgatg ctcactggcg gcggtggagt
    30301 ggcgagatga gcgaaattgc gtctggcgtt ttacgtcagc aactggattt gattgccacg
    30361 cgcaccggag aaaataaatg gctgacgcgt gagcaaacct ctggtatgca gcaacaaatc
    30421 cgccaggctt tgtcggcgtt accattgccg gttaaccgac tggaagaatt cgataactgc
    30481 cgtgaggcgt ggcgtaaatg tcaggcctgg ttgaaggaca ttgaaggcgc tcggttgcag
    30541 cataatcagg cgtataccga agccatgctt accgagtatg cggatttttt ccgccaggtc
    30601 gagtcttcac cgctgaatcc ggcgcaggcc cgggcagtcg ttaatggcga gcattctctg
    30661 ttagtgctgg caggtgcagg aagcggaaaa acgtcggtgc tggtggcccg tgctggctgg
    30721 ttactggcgc gtggtgaagc gtcccctgag caaattttat tgctggcgtt tggtcgcaaa
    30781 gccgctgaag agatggacga gcgtattcga gaacgactac ataccgaaga cattaccgca
    30841 cgcacgtttc atgcgctggc gctgcatatt attcagcagg gcagcaaaaa agttccgata
    30901 gtcagcaaac tggaaaatga taccgctgcc cgtcatgaac tttttattgc tgagtggcgc
    30961 aagcaatgca gcgaaaagaa agcgcaggcg aagggctggc ggcaatggct gacggaagaa
    31021 atgcagtggt cagtgccaga aggtaatttc tgggatgatg aaaaattaca gcgtcgtctt
    31081 gcctctcgcc tcgatcgctg ggtaagtctg atgcggatgc acggtggtgc acaggcagaa
    31141 atgattgcca gtgcacccga agagattcgc gatctgttca gtaaacgtat caagttgatg
    31201 gccccgttat taaaagcctg gaaaggtgcg ctgaaggcag aaaacgctgt cgatttttcg
    31261 ggccttattc accaggcgat tgtgattctg gagaaaggtc gctttatcag cccgtggaag
    31321 catattctgg ttgatgaatt tcaggatatc tcgccgcagc gtgcagcgtt gttagcggca
    31381 ttacgcaagc aaaatagtca aacgacgttg ttcgctgttg gtgatgactg gcaggcgatt
    31441 taccgattca gcggtgcgca aatgtcgctt accacggctt tccatgaaaa ctttggtgaa
    31501 ggtgatcgct gtgatttaga cacgacctac cgttttaaca gtcgtatcgg tgaggtggca
    31561 aacaggttta ttcagcagaa cccaggccag cttaaaaagc cgctaaacag cttaaccaat
    31621 ggagacaaaa aagccgtcac gttattggat gagggtcaac ttgacgcttt gctggataaa
    31681 ctctctggtt atgccaaacc ggaagagcgc attctgatcc tggcgcgtta ccatcacatg
    31741 aggcctgcca gcctggaaaa agcagcaaca cgctggccga agttgcaaat cgactttatg
    31801 accattcatg ccagcaaagg gcaacaggcg gattacgtca tcatcgttgg cttgcaggag
    31861 gggagtgatg gttttccggc tgcggcgcgg gagtcgatta tggaagaggc gctactgcca
    31921 ccggttgagg atttcccgga cgctgaagaa cggcggttaa tgtacgtggc gctgacccgg
    31981 gcacgccatc gggtatgggc actgtttaac aaagagaatc cctctccctt tgtggatata
    32041 ctgaaaaatc tggatgtgcc ggtggcgaga aaaccgtaag aaacaggtgg cgtttgccac
    32101 ctgtgcaata ttacttcaga cggtctgcga gataacgctg ataatcgggg atcagaatat
    32161 cgaccgcgtc gttaaaatgc ggtgactgga taatgaagtc tgccgttgcc acgttggtgg
    32221 cgaccggaat gttccatacc gttgccagac gcagcaaggc tttcacgtca ggatcgtgcg
    32281 gcacggcatt tagtgggtcc cagaagaaaa tcaatacatc aattttccct tctgagatca
    32341 atgcgccaac ctgctggtca ccccccattg ggccactcaa catcgcgttg acgttcatgc
    32401 cggtcgcgcg ggaaattaag ttaccggtag tgcctgttgc atacagtacg tgttgttcca
    32461 gtaacggttg atgccgttcc acccagctca tcagcatttg tttgcagtga tcgtgtgcca
    32521 ccagcgcaat atgtttccgc gcaggtaaag tgcgtgtcgt cagttccata atgtacatcc
    32581 gtagttaact ttcctacaga ttactgtaag cacttatcgc tgcaagataa agaccgaaaa
    32641 agcctgcgca caggcacaaa aatctcagga agatggttgt ttttccgccc attgcaggaa
    32701 agtatttcgc gtttgtgggt cagccagttt aaaccaatac ttcagccgtt gttctgtgag
    32761 cacctgagac tgcggtggaa tagcatccag ctcggaaaca cccgaaagca gcgtactatc
    32821 atctgccatc atcgtggcaa attgcggtag tgaagcgcgt ttcgcggatt tgttatagcg
    32881 ttcaacctcg acctcataat caatcgtttt tgccgttgag gtaatggcga gaatatccag
    32941 ctttaccgga atcggcgtcg catcgccatc caacaattca aggcgcggcg cggcatcaaa
    33001 atggttagct tcccgctcat tctccagacg aggcaggcga aaattgacct ggttgatgag
    33061 ctgggtatta aaactaacca ccagtggagg ggagatatag agccgttctt cactattgga
    33121 aagatgaatc gtcttctcaa cgcgaaacac taactgatgc ggtccattgt ccagttcaat
    33181 gctgtccgca ccgcgcagca gagagctgga aaccttctta ccgtcgagta ccagaagatc
    33241 gacatcggtt gaaagccgca gcgtggtggc aaaaacggat accggcaaac ataatgcaat
    33301 caaggtggtc acgatgccgg ttttcataga tagctcctgt caaaagaccg cccgaataat
    33361 aatgtgtcaa aatttttctt taatcatact tactaacata tagacatatt tcccgctttt
    33421 gctctcattc attcgtatta gctgcatggt tggcatgtcg gctttgtcgt acactttgtt
    33481 aaacagtctg gaggaaataa tgaaagaaac cgatattgca ggcattttaa cgtccactca
    33541 caccattgcg ctggtggggg caagcgacaa acccgatcgt cccagctatc gcgtgatgaa
    33601 atatctactc gaccaggggt atcacgtcat tccggtttcg ccaaaagttg ctggcaaaac
    33661 actgctgggc cagcaggggt atggcacgct ggcagaagtt cctgaaaaag tcgatatggt
    33721 cgatgttttc cggaattcag aagcggcgtg gggcgtagcg caggaagcga tcgccatcgg
    33781 cgcgaaaacc ctctggatgc aactgggggt gattaatgag caagcggcag tgctggctcg
    33841 ggatgccgga ttaaatgtag tgatggatcg ctgcccggct attgagatcc ctcgcctggg
    33901 cctggccaaa taaaaaatcc ccggaaggca aaaaccttcc ggggatttgt tcagggatta
    33961 gttacgcaga cgcggggcct ggagttgttt acggatggtc tgcgccagct catccatgga
    34021 tggctgttca ggatgttcat cctgcaattc gctacttagc tgggcttcag ccaggtaggt
    34081 atgaaccggt aggccgttat cgtcctccat caccacgtga taccacggag cggcgcgaag
    34141 ctcgtcattc accgccagct catcaggcga cggttcagag agcgaataaa ccgggtcgat
    34201 atccacgacc actccgagat aacctaacag ggaatggcgg acctgctggc cgataccgaa
    34261 tttgctggca atcatagtca cctcccggga aatctttacc tttgatataa gggtaaaatt
    34321 ccacatttca agttacatga cgcgacaggc aaaccctttc agatatagcc cttccggata
    34381 ggtagcgatc accggatgat cggctgcctg acggaactgc tctataaatt gtacatcacg
    34441 accggcatca attgcggcat ccgcgatgat tttctgaaat aaatcgctgg tcatcagacc
    34501 ggaacaggag aaagtcagga gaataccgcc ttcattcagc agctgaatcg ccagcatgtt
    34561 gatgtcttta taaccacgac acgcgcccat caactggctt ttattctcaa caaacttcgg
    34621 cgggtccatc acgataacgt caaatttttc accgcgatcg cgataagtac gcagcaattt
    34681 aaagacatca tcacggacaa actcagcctt gctcagatcc agtttgttca gctcaacgtt
    34741 ctgccgtgca atatccaggg cttcctggga ggtatcaacg ctgacaacct ggctgcaacc
    34801 gcccatcagt gccgatacgg cgaaaccacc ggtataggag aaacagttaa gtacgcgttt
    34861 attttcgacg tagcggcggg tagccaggcg gctgtcacgc tgatccaggt agtagcccgt
    34921 tttgtgtccg tgctgaatat tcaccagcag tttcatgccg tgttcttcaa tcggcagcag
    34981 ggcaggcggc aactcgccgg tgatggggcc ctgagtcagc tccatccctt cttttttacg
    35041 taccgcgacg tcgctgcgat cgtaaatggc acattccggg tacagcgttt gcagggcgct
    35101 aattaatgcc gcgcgctgat actctgcgcc agcactcagc agttgcagca ccagaaaatt
    35161 accgaaacga tcgatagtaa tacccggcag gccatcagat tctccggcga ttaaacgata
    35221 gctgtcgagg ccatcttttt gcgccagcca gtcacgccat ttttgtgctt gctgcaaacg
    35281 gcgggtaaaa aaagcaatgt cgatagattc attcgggtca aacgtccaga cgcgcgcccg
    35341 gatttgcgaa gctggcgaat aagcgccgcg tgctaaccat tttccctgat gatcaacaat
    35401 atcgatggtt tcaccgaggc tggctttacc ttccatgcgg gcaacggccc cggaaaagac
    35461 ccacggatgg cgacgaagta atgatttttc gcgccctttg gctaacacta aacgtacact
    35521 cataatttac ttttctgtcg atgcaaagaa atgggcgtca ttgtccggag ttttccatgg
    35581 atttgcaacg cgtccattaa ggataaggag agcagcaatg tcgaaagtct gcataattgc
    35641 ctgggtttac ggtcgagttc agggcgttgg atttcgctat accacgcagt acgaagcgaa
    35701 aaaactggga ttagccgggt acgccaaaaa tcttgacgat ggcagcgtgg aagtggttgc
    35761 ctgcggtgaa gaagggcagg tagaaaaatt aatgcagtgg ttaaagagtg gcggcccgcg
    35821 ttctgcgcgt gtagaacggg tgctcagcga gccgcatcat ccctcggggg aattaaccga
    35881 ttttcgtatc cattaaatac attttaccgg ttttggcagg ccagcaattt tggtggcttg
    35941 cttagccgga cctttcggaa acagtcggta taaatagcgg ctgttacctt tttcttcgcc
    36001 aaatttattc gccatcgctt ttaccagcat acgaatcgcc ggagaagtat tgaattccag
    36061 atagaaatca cgcacaaaac gcaccacttc ccagtgttct ggcgacagcg aaatcccttc
    36121 gttctctgca atcaccactg ccagcggctc actccactgg ctgctttctt tgagatagcc
    36181 ttcggtatcc gtttctatct ctttaccttc gaagatcagc ataattatta ctaccttaat
    36241 cagactggcg gcagtgtaac aaaaaacaaa gccccgcata aagcgaggct cggtaagtaa
    36301 ccctgggcga gattaatcgc ggctggcgaa gcccagaatg ctcagcaggc tgacgaagat
    36361 gttgtacagc gaaacataca ggctaaccgt ggcacgaata tagttcgttt caccgccatg
    36421 aatgatgttg ctggtttcaa acaagatagc gccagaggag atcagaatga agaccgcgct
    36481 gatcgccaga tgcagagcag gcagttgcag gaagatatta gcaaccatac caatcagcac
    36541 caccacaata cccgccatca gcataccgcc aaggaatgac atatctttgc gggtggtcag
    36601 cacatatgca gagcagcaga agaacactaa cgccgttccg cccagtgcca tagcgattac
    36661 gtcacccatt ccggtagcca gataggtatt caaaattggc ccgaggatat aacccagaaa
    36721 accggtaaag gcgaatgcgg agataatccc ggtcggctta ttcgccgttt tgtaggtcag
    36781 gaacatcaaa ccatacatac ccaccagcgt cagaatcaga cccggagatg gcagcatcag
    36841 caccgtactg gcagttgcgg taatcgccga aaaggccagc gtcaggctca gcagaaaata
    36901 ggtattacgc agcaccttat gggtgctaag cagcgatgta cggtcatgtg aagaactaac
    36961 tatacgatcc attgagtcac tctctatgac agatgtaatt aattaagcag cataatgata
    37021 atgtgggaag gtacacagaa gttttaccca tctttacgca tttgatctga aacaagtttc
    37081 acagcggagt ctcaggtcat taggcagtga tataacgccc tgagaatttc gacaggcaaa
    37141 agaaaaaggg gttagcattt agctaacccc ttatcttatt tggcggaagc gcagagattc
    37201 gaactctgga accctttcgg gtcgccggtt ttcaagaccg aagaaaaata aaggaaaatc
    37261 agtataatac gtaaaatagc tgaaaagtta atcagactta acaccaagaa aaatcaagat
    37321 gttagggagt tattcagagt taatatacca ggtctttttg ccagaaaatt ttctgtattg
    37381 cctacttact gaccacaagc ggcaaatcca gtgttggcgt gattttggtt ttacgatcgt
    37441 aaatcaacac ctgattttct gttttgtgtc cactgaaaat ttgtttgtcg cgactgctgc
    37501 cttcgtaatc tgaaatccct ttggctttta tgtcatggaa gttgcaccca aacggaacgc
    37561 cggctttttg ctcggctgca cgtttagcct gattccacca gttgttcagc gtcttggcta
    37621 ttaccttccc gcctttggtt gtgttgatca catattcgca tgtgccggaa gatacatttc
    37681 gggctaactg gatcgccgta cgtaatcgtg gagaccattc cttgatttgt ttggtgccgg
    37741 tcttgttttg ctcaatgtaa atccctttgt ccataatatc ctgccatttc agctcgagca
    37801 catcaccgag ccttgccgca cagagatagg atatctccat tgcaatacgt aactgtggaa
    37861 ttgcttccgc atatatcgca gcatactgtt catcggtgat gtaaacagta cgggctttaa
    37921 gcgtgaattt tctgactcct ttgcatggat tagttttcac atacccacgc tcatatcccc
    37981 atccgtatac gcgactcaga cttgccagtt catggtttgc ctgggtcttg ctctcaagac
    38041 cccgcttatc catgaaaatt cttacctgct caatttttac attatcagca agcattttcc
    38101 cgaataccgc cagtaatgcc ctttgatgtt gccgataatc tttttgggtt cggggggcca
    38161 gttctgtaaa tgcaggggag tccataaaca tgtgccataa tttagctacg gtcattatgt
    38221 cgtggagttt tgccttttcc agttcataat tttgccagac tttagccacg ctggtttccc
    38281 gcactcttcc gagctttatc gttcttgtgc ttcctttggg cttccatacg taactgtaac
    38341 cattcgatct aacgcgcggt ggcagtacat tatctttttt gtcttttctt ggtcttccca
    38401 ttattcagtg cctcaaaatc ggggtcagca gaaaccagtt cagatacttt tgacattgct
    38461 gttagtccac gtggaatatc acaacgtaga actatcggct cgtttttagg cccgattaaa
    38521 aatgggatgc cgtgcagtct taactggtgt tgctgtcttg tgtatcgctt gtatttcgtg
    38581 atctcttgaa tctctgctgg cgatagagtt aattcataca tgtggtcacg tgccttacag
    38641 catgaccgcc gccaatataa ttcggagacg gcgatcaggg ttgaacatta aaaatcagcc
    38701 tgattcggga tcagtttttg aatagtggct gtaacgtatt ttgcctggtg aagggcgtca
    38761 tccagcgcat tgtggggtac gccttcgaat ggaattacgc ttctggcatt gaagtctatg
    38821 gttttcccca gctcaacgat cgtgcgtaca tcacgatcgt tgctgtaatg ccacgggcag
    38881 gggatgccca ggcgttcgta tgaggtgcgc aaaattgagt tatcgaacgt tgcaccatta
    38941 ccccatacct gaacgaattt ttcatctgag tattcgttga tgaactcccg aaaccgcgaa
    39001 agggcatcct tcagttttac ctggtctgtt aaaatggcag ctctggcttc actggactgc
    39061 ttcagccacc attcgatggt tccaccgtca ggaacagccc ctgtattcat tgcgtcagtc
    39121 agactgataa cgatataaaa tactggcccg atttcccctg tttgtgggtc gaagaaaacc
    39181 gcaccaataa ccacgatggg cgcattggtg ttggttccca ttgtttcaag gtcgatcatc
    39241 aggtggtacc acactctgct ggtggatgtg ataatgtgat gaccgttcac cgcaattaag
    39301 ggagctgccg tctcgccagt ttcattatcg ctggcgtggt cctgagcgct gccagcattc
    39361 tccttgtgtg gatgttcagc gccttccatt tcctccggat cattttcctg aacttcaacc
    39421 tgattctctt catcgaatgt ttcctggtat gttgcgtcgc ccatcaccgc gccacaatca
    39481 gggcagttgc cgccaccgct ctgaccgcag gcggtgcaga ctttttccgg ctcctgttgc
    39541 gctactggtt caggttgttt cgtttctggc tcgttttgta acgcattttg gctgttttgt
    39601 tccgctttct ggtcgttctg ttccgtttct tgctggttct gattcactga atcgcgggtt
    39661 tcaatcccct tcacccattt cggatcattc gggtcgctaa tccctgcaac aaattcacca
    39721 cgtgatgcgg cgagcaactg attggcgtca ggctggctga tattggctgc ctgcataatt
    39781 ttctttactt cgtcagcggt aacttttacc ggctctggtt gtgcggtcgt gtcagatgca
    39841 ccagtatttt gttgtaaacc tgagtatgta ccgtttttgc gggcaaaata ttcttctttc
    39901 gtgatttcag tagcccctgc agtcagcgcc ttattcagac cagaaagttt gtttgcgcga
    39961 ccgtattttt cgccatcctt gtcggtgaag aggaagtaga acggcccctc acgctctaca
    40021 gatggttcga tttccacttt gcattcggtt ttttcgttgt ccggaattgc cgtttccact
    40081 gcatcagttt ctggtactgg cgatgagaga gtatcagttg cgctctgatt tgttccttca
    40141 tcttcaaaca cgccctttgt agtcaggtat tcagtaatgt atttgttcag tgccacaggg
    40201 tctttgtgaa tgtcgatcgg acgttcacgg acaaggccaa aaatagtctg gcggtcgtag
    40261 cgaagggcat caggctgttt gcgcattgat gccgagatac gcttccagtc ttcacggtcg
    40321 ttgtcgataa cttcattttt tgcccagcga tggatgctgc cgtcaatgtt tccggcatcc
    40381 acatcaccag gccagagagc gtaggccagt tcgtcatcca gtgttttcca tgtctgcttg
    40441 tattcgcgat gaatggcagc aatgaccggg ctgatttttc ctgttgaatt ttcagtgtgc
    40501 tgttgattgg ctcttgcgcg ggcgagatca acaacagacg tgtattttcc ggtttccttg
    40561 cgttcacctt cgcgacgttt tttccagatg cgcatctctg cctgaatttc gggccatttg
    40621 gcacctggct tacatttatg cttaacccac ccgatggcat gcagcttaag ctccggatac
    40681 atggcgttaa cttctggcat tttcatcaac gcttcaacga tatgtccgtc gaatgttgcc
    40741 atgtcttcct gcaacagttc ctgcgcacta atcaccatat caacggtgat gttttcacat
    40801 gtgtcgaact taaccatgac agcgttctgt acttcagggg ccagcttgtc aaaagtgacg
    40861 ttcatcggat ctgattcagt ctcaaccggg acaaaggaag cagagtcctc atcccagcgg
    40921 ttttcctgca tatattcagc atcccaggaa tcgagggcag ggcggggtat accgggttta
    40981 tcctcgcaaa caagaaattt ataagcgcag tcctgagcag ccggatattg ctccaggaat
    41041 tgccaggtaa atttggcacg ggcgcggcgt tcgtcaccgg cttcaatggc agtggctaca
    41101 gcaattgcac cttcttcctt tattgcctgt tcgtccggaa tggcggcgca aataaagact
    41161 ttactcattt tgttttaacc tcatgacata tttaaggatg aacaaatccc tgccattgct
    41221 ggcatataag aatcaaatct gatgtattca ttaagctgaa tgtcgtattg tggcagttat
    41281 tttattactg ctcaccatga ctctgctttt acaggtaaac catcacgacc aaggaagact
    41341 ttaatcatgg tttccttaat acagtgttgt gtggaaaaat cacgaatata gagccgttgt
    41401 tttttaatgt tgtttaccga agcaatatat gttcttcctt tatgaataac ataatcaccg
    41461 ggggtcacgc actgacgagg aatctcatca gttccgaagt gatgagcaat cataattatc
    41521 tccattttta caaatgaact ttgttgatgc ggtgcctggt gcctccaggt gacgttaacc
    41581 agttaacaat taacgccgga tacagagaat ccacccataa cactgttttc ggttttaact
    41641 gttccgcgtg cgctcagccg cattcaccac atcacaaaat tcactttaaa aagggcggca
    41701 gagcagtcac ggagtaaaac tgataccgcc aaacgtcacc agaaaattga taacagaggg
    41761 cgttgcagcg gggttatcac ttaagcgtat ggtcaacctg acaacccggt gtcctcaacg
    41821 gggaaggaat aaccccgcca tacttaccgc cgcgccattt cgcggattgc cacaaccgga
    41881 agcgcacggt cgacgaaaat ttaacgacag accatttatg aactaacagc ctcgccgtgc
    41941 gctttcgcgt tatgccctga cttttcaggg acatatcctt tcagtaaact gtcagtgccg
    42001 gatgctcacc cgtgtccggc gcacgcactc cacctgaccc gtggagaact ccttaattac
    42061 caaccctcag gagggtgaaa tggataaaaa gcaaattgag gccctgcaat ctattattga
    42121 aaaacaagat gaagctatca ggattctttc atatcgcact gatatgatac taaatatgct
    42181 ttctgcatta acggctgcgc ttggtggtac aaaaacaaac gtataccgcg aagttgttat
    42241 tcaacagata gataaatttg aaaaaaccat accaggtatt aatgctcatc ttgcagaaca
    42301 agagaaagac catgctctta tggcaatttc ttcagtagct ctcccgaaag ttgagtagtt
    42361 ttaattgttg ttttgaaata atcactgctt tcacatttga gtgatttcat ggcaatccaa
    42421 atgcgggcct ctgtgcctgc atttggttcc agttgctgta gacgttttgc gtcttccaaa
    42481 agtaaggcga taatgtgttt cagcttctca tcatttgctt gattcttgtt ttcaggcgaa
    42541 ttctgtccgc cgaatgggcg cttctcttca tacagaccta taaaggcacg acgcacgtta
    42601 ccggatatag tatcgatggt ttccttttct acagtactca ggtcaagagt cgccagttgg
    42661 gaacgaacta tattcgctgc catttcctgg aatggcattg gtaaatcttt aaattccatc
    42721 gtcagcctca tcagtcagta gttttcaatc actcgtgaaa cgtttaatgc cgcgcttttt
    42781 gcaagagtgg taatatcctg ctccgctgac gccagttctt tagagaggcg ctctttatag
    42841 tcagcagcct gcttcaagtc attaatggcg cgtatcttcc cgcacatcca ttcgtaaatt
    42901 tcatcatcgg tatagcctgg tgcgatgatg atgggttctg gtttctgcat actgattcct
    42961 cgcggtgctg tttcgcttat cagtcattag attttgccga actggaaagc gcctgtttaa
    43021 actcactgaa gctgagagct tcttcgcctt cggcaaggcc ttcgaagtat tcttcgtaag
    43081 ccttttccat gattgcgtcg aaatccatat cactcacctg aatttctttc cagccagcga
    43141 cgcgcgccag attctgtttt aaacgttttg cttttggtat acgtcatcgc ggtgaacgtg
    43201 ccgtcctggt tgggaaacac gccgcacacc agagattcgt tgttgccaag atcgatagta
    43261 tccatgctga cctcatttcc ccttaacgcc gggggagcgg aacaaaaacc tgctgcatag
    43321 ttattaaagt tgaaccctgc cgtcatgttc ttacgcctcg ggctggctac ttaccccctg
    43381 accactgcct ggtaactcaa agtattgccc tgcattctgt ggggcggggt gggttggtat
    43441 gagaaaacta taggtaatgc ctaattactt gtcaataggc tatgcctaat gctttttgcg
    43501 cacaacctaa taggcgatgg tatgtggaag aggtaatggg agctaaataa cgggatctag
    43561 gtgcttcccg tcggaccata taagtttaag ttcccgtttt ggtgatgttc tggcttttcc
    43621 gttctgattc ttgatttttc agatagttac ttaccttcat ttccattgca gtaatgtagg
    43681 cgcgaacgtc ataatcaacc caaccaggct ccgcagcatt tccagataag aggaaggcta
    43741 caattgctct tttttcatca gaagcggctt gataaaggct gtttatgtct aaaagttctc
    43801 tttttgaatc tgagcaggaa ggggttggta tggggtattc gttaagcccc caatgctctg
    43861 gaccaaccac atcagaaaag aaacgccata gttctggaag tttatcttta cttatcgaac
    43921 ctttcttaat ccagtcatgg attgatggtg gttggacttt gaaatggcgt gcgatctccg
    43981 cctttgattt gacggctcct gatgcaattt ttttattaat ggcctgctct atcgctcggc
    44041 ctaagtcttt accactaagc attgcttaat agtctcctat gtgcattgca ttaggcaatc
    44101 cctacctctt ggttgttagg catagcctat tgacaatgat attaggcgtc gcctaatatc
    44161 attgtgtgtt ttttggagtt cattcgatga aaaaagagaa ctattcattc aaacgagctt
    44221 gtgctgttgt tggtgggcaa tcagcaatgg ccagactttt aggggtatcg cctccaagcg
    44281 taaatcaatg gatcaaaggt gtacgtcggt tgcctgccga gagatgtcca gcgattgaac
    44341 gagcaacaaa aggtggagtc ctgtgtgaag aacttcgtcc tgatgttgac tggtcatact
    44401 tacgacgttc gtcatgttat tcgcagaata tgtcgatgaa gcaaccaaat gacaaaaacg
    44461 atcatacccg aagcatcaag aggcaaatga ttcatgaaaa tcaagcatga acacatccgc
    44521 atggcgatga atgcctgggc gcgtccggac ggtgaaaaag ttccggcagc tgaaataacc
    44581 cgggcttatt ttgagctggg tatgacattc ccggaactgt atgacgacag ccatccggaa
    44641 gccctggctc gcaatactca gaaaattttc cgctgggtgg agaaagacac ccctgatgcg
    44701 gttaaaaaaa ttcaggcttt gttaccagcg atcgaaaagg caatgccacc tccgctggtg
    44761 gcctggatgc gcagccacag ttccgattat tttcgggaac tggtggatac gcatgaacga
    44821 ctggtgagag acgctgatga acttgtcgcg gtggtaattg ctggtttcaa tcagatgaat
    44881 cgtggtggtc cggcgggaaa tgccgtggtg gcgcattgat aacgtgttct gggggagggg
    44941 atgaagctcc tttttgctga acgcccgctg gttataaaca cgcagctggc gatgaaaatt
    45001 ggtctgaacg aagccatcgt gttgcagcag ctgcattact ggttgagaga taccggttcc
    45061 ggcatggaat gtgatggtgt tcgctggatt tataacacaa cagaacaatg gctggaacag
    45121 ttcccgttct ggtcagagtc aacgttaaaa cgcgcatttg caagtctgaa aacgctgggg
    45181 cttttgcgtt gtgaaaagct caacaaatca aagcgtgata tgactaattt ttacacgatt
    45241 aattacgaga gtgagctttt agatggtggc aaagtgagcg aatccatcag gtcaaaatgc
    45301 accgctccat caggtcaaaa tgacacgatg gaagaggtca aaatggcacg ctccattggt
    45361 tcaaaacgac ccaatgtcat cgggtcaaaa tggcctgatg atcttacaga gaatacaaca
    45421 gagattacta cagagaataa aaacacttct cgtccggaag cttcgcaacc ggacccgcag
    45481 acggctgaac aggatttttt aacccgacac cctgacgcgg ttgtgttcag tgcgaaaaaa
    45541 cgccagtggg gaagtcagga agatttggcg tgcgcacagt ggatctgggg acgaattgtg
    45601 agtctttacg agcaggctgc cagcgatgat ggcgagatct cgcgaccgaa agaaccaaac
    45661 tggaccgcat gggccaatga tgtgcgcaca atgcggatgc tggatggcag aactcacaga
    45721 caaatttgcg aaatgtttgg gcgggtacag cgggatccat tctgggtaaa aaacatcatg
    45781 agcccgtcaa agctccgcga aaaatgggac gaactggtca tccgcctagg gcgttcacct
    45841 gtacagcgtt gtgtgaatca tatttctgaa ccagacaccg aaattccgcc ggggtttagg
    45901 gggtaacgaa ctgtgaaaaa tattgcgaca ggcggtgttc ttgaacgtat ccgtaagctg
    45961 actccgcagc atgtaaccgc gccgtgccgg acagtggacg agtggcgaga gtggcagctt
    46021 gcagaagggc gaaaacgtag cgaggagatc aaccgcctga atcgtcagtt gcgggtggaa
    46081 aaaattctga atcgctcagg catccagccg ttgcatcgta aatgctcgtt tgcaaattac
    46141 caggtgcaga acgacggcca gcgatacgcg ttaagccagg cgaaatccat cgccgatgaa
    46201 ctgatgaccg ggtgtacaaa ttttgcgttc agcggaaaac ctggtaccgg gaagaaccat
    46261 ttagcggcag ctatcggaaa tcgcctgctg aaagacggtc aggcagtgat tgtggttacc
    46321 gtggctgatg ttatgagtgc cctgcatgcc agctatgacg acgggcagtc aggcgaaaaa
    46381 ttattgcggg aactgtgcga agtggatctg ctggttcttg atgaaattgg cattcagcgc
    46441 gagacgaaaa acgagcaggt agtactgcac cagattattg atcgacggac agcgtcgatg
    46501 cgtagcgtgg gaatgctgac aaacctgaac tatgaggcca tgaaaacatt gctcggtgag
    46561 cggattatgg atcgcatgac catgaacggc ggacgctggg tgagttttaa ctgggaaagc
    46621 tggcgcccga atgtcgtcca gccaggaatc acaaagtaat ttttaccagg aaaaaaattt
    46681 aatggagact gtttttgacg cactgaaagc aatgggaaaa gccacatcca tagaacttgc
    46741 ggcgcgactt gatgtcagtc gtgaagaagt gcttaacgaa ctatgggaac tgaaaaaggc
    46801 tggttttgtt gataaaagcg cgtacacctg gcgtgtggct gataacaacg ttcagcagga
    46861 acagccagcg caggcagagc tgccggaaga aaccaccaca gcaacagtag cgaaaatctc
    46921 agagtgcgat ttaaccgcga cgattgaaca acgcggacca caaacggcgg atgaactggc
    46981 tacgttgttc ggtaccacat cacgcaaagt agcttcaacg ctggcaatgg caatcagcaa
    47041 agggcgtctg attcgcgtaa atcagaacgg taaattccgt tactgcatgc cggacgataa
    47101 tttaccagca aagccgaaag ccgtatcggt aacggaaacc gatggtaaag cctttcctca
    47161 gccagccggt gttgcgttac cggtacagaa agatgcaaca caggaagata ttaaaacaga
    47221 aactgtggcg gacattgtgc agtcgctgcc atcgtttact gcaacgcgag aagatgattt
    47281 gattttgcca tcgctgcata tggcaaatcg cgaactgcgt agggcgaaga atcatgtcca
    47341 gaagtgggag cgagtctgcg ccgcgctgcg ggagttgaac aagcaccggg atatggttgc
    47401 cgggatttgt cggaagtccg ggcaatgagt ggatggtgca ggcctgaaat catgatacta
    47461 acaatgaagg taaaatgcat cggcagtctg attggtcgta gtgaggcggc ggtcaggatg
    47521 aaatcccggg ttaagggaat aagcctgatt ctgcggggtg attttcacca gtcaacaaaa
    47581 tatccgtagc gcgataacgg tcaaaaatca tggcgctgac acttttgtgc cactggagat
    47641 gactgtacct aagttcaggg gagaagaaca cgtccggtgg gatggtcggg ccagatttaa
    47701 agggcaggtc atggctccag cctgtacgct ggcaatggag gctgcctagc gggaaattga
    47761 tatgggaacc acgccactca gggatttact gccggtccag agaataaatt cctgttacgg
    47821 ttacaccact gtgatcttgc aagtgcagga aagtaggtct acacggtaac gcgagtgcgt
    47881 gtgacttttg atgtcattcc cgtagaaaca ccggacaaat tttcgctgac aggtcatgct
    47941 gaaggtataa atctgcagat tatggacaat tacggatatc cggcaagagc cggaaaaagc
    48001 atgccgcctt taattctcag tggaagatgg acttgattat actcattgca ttgtcagaaa
    48061 tagttatcca ttaaaggctg gctattccaa acaggatgtt gattacaaaa atgtaatcaa
    48121 catgtaaggt ttatactctt caatatgcgt ataattttcc ttattttgtt aactttaaat
    48181 aac
//
LOCUS       DAZFJL010000017        47188 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFJL010000017 DAZFJL010000000
VERSION     DAZFJL010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.017"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(184..264)
                     /locus_tag="ACHJ8C_000978"
                     /pseudo
     CDS             complement(184..264)
                     /locus_tag="ACHJ8C_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(259..364)
                     /locus_tag="ACHJ8C_000979"
                     /pseudo
     CDS             complement(259..364)
                     /locus_tag="ACHJ8C_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
     gene            544..717
                     /locus_tag="ACHJ8C_000980"
                     /pseudo
     CDS             544..717
                     /locus_tag="ACHJ8C_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            828..1751
                     /gene="yicL"
                     /locus_tag="ACHJ8C_000981"
     CDS             828..1751
                     /gene="yicL"
                     /locus_tag="ACHJ8C_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO5550878.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSMLIARYGTLPVVGWSMLIGGLILLPFYAGQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(1755..2573)
                     /gene="nlpA"
                     /locus_tag="ACHJ8C_000982"
     CDS             complement(1755..2573)
                     /gene="nlpA"
                     /locus_tag="ACHJ8C_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO5550879.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQTHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            2795..3088
                     /gene="yicS"
                     /locus_tag="ACHJ8C_000983"
     CDS             2795..3088
                     /gene="yicS"
                     /locus_tag="ACHJ8C_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO5550880.1"
                     /translation="MKPTMLLMITVFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(3129..4319)
                     /gene="nepI"
                     /locus_tag="ACHJ8C_000984"
     CDS             complement(3129..4319)
                     /gene="nepI"
                     /locus_tag="ACHJ8C_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO5550881.1"
                     /translation="MSEFIAENRGANAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            4553..4906
                     /locus_tag="ACHJ8C_000985"
     CDS             4553..4906
                     /locus_tag="ACHJ8C_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5550882.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADVLGGGVFKKR
                     LHKNRERAIILAKGGSNWFYTFLYAKQDMSNINSQELAGFRELAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            4890..5213
                     /locus_tag="ACHJ8C_000986"
     CDS             4890..5213
                     /locus_tag="ACHJ8C_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5550883.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVDAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(5329..5781)
                     /gene="yicN"
                     /locus_tag="ACHJ8C_000987"
     CDS             complement(5329..5781)
                     /gene="yicN"
                     /locus_tag="ACHJ8C_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO5550884.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(5834..7168)
                     /gene="adeQ"
                     /locus_tag="ACHJ8C_000988"
                     /gene_synonym="yicO"
     CDS             complement(5834..7168)
                     /gene="adeQ"
                     /locus_tag="ACHJ8C_000988"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO5550885.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGVFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALSLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALCVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVATLFALKIILVD"
     gene            7343..9109
                     /gene="adeD"
                     /locus_tag="ACHJ8C_000989"
     CDS             7343..9109
                     /gene="adeD"
                     /locus_tag="ACHJ8C_000989"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO5550886.1"
                     /translation="MNNSINHKFHHISRTEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIDRQP
                     VSASDFALQFTPGKRYRVIEVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEFTE"
     gene            complement(9156..10547)
                     /gene="uhpT"
                     /locus_tag="ACHJ8C_000990"
     CDS             complement(9156..10547)
                     /gene="uhpT"
                     /locus_tag="ACHJ8C_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO5550887.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(10685..12004)
                     /gene="uhpC"
                     /locus_tag="ACHJ8C_000991"
     CDS             complement(10685..12004)
                     /gene="uhpC"
                     /locus_tag="ACHJ8C_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO5550888.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(12014..13516)
                     /gene="uhpB"
                     /locus_tag="ACHJ8C_000992"
     CDS             complement(12014..13516)
                     /gene="uhpB"
                     /locus_tag="ACHJ8C_000992"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO5550889.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLMQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQHDERLMLVIE
                     DDGSGLPPDSGQHGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(13516..14106)
                     /gene="uhpA"
                     /locus_tag="ACHJ8C_000993"
     CDS             complement(13516..14106)
                     /gene="uhpA"
                     /locus_tag="ACHJ8C_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO5550890.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(14179..14469)
                     /gene="ilvN"
                     /locus_tag="ACHJ8C_000994"
     CDS             complement(14179..14469)
                     /gene="ilvN"
                     /locus_tag="ACHJ8C_000994"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO5550891.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(14473..16161)
                     /gene="ilvB"
                     /locus_tag="ACHJ8C_000995"
     CDS             complement(14473..16161)
                     /gene="ilvB"
                     /locus_tag="ACHJ8C_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO5550892.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVSASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(16267..16365)
                     /gene="ivbL"
                     /locus_tag="ACHJ8C_000996"
     CDS             complement(16267..16365)
                     /gene="ivbL"
                     /locus_tag="ACHJ8C_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO5550893.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            complement(16610..16684)
                     /gene="ysdE"
                     /locus_tag="ACHJ8C_000997"
     CDS             complement(16610..16684)
                     /gene="ysdE"
                     /locus_tag="ACHJ8C_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO5550894.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            16766..17950
                     /gene="emrD"
                     /locus_tag="ACHJ8C_000998"
     CDS             16766..17950
                     /gene="emrD"
                     /locus_tag="ACHJ8C_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO5550895.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLIPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(17958..18455)
                     /gene="yidF"
                     /locus_tag="ACHJ8C_000999"
     CDS             complement(17958..18455)
                     /gene="yidF"
                     /locus_tag="ACHJ8C_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO5550896.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQLGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDTTLQKWCGSEKS
                     QPRRDCQACDWHRLCPHAREDKPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(18452..18814)
                     /gene="yidG"
                     /locus_tag="ACHJ8C_001000"
     CDS             complement(18452..18814)
                     /gene="yidG"
                     /locus_tag="ACHJ8C_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO5550897.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(18804..19151)
                     /gene="yidH"
                     /locus_tag="ACHJ8C_001001"
     CDS             complement(18804..19151)
                     /gene="yidH"
                     /locus_tag="ACHJ8C_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO5550898.1"
                     /translation="MKISRLGEAPDYRFLLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTKSLFIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(19211..20704)
                     /gene="yidJ"
                     /locus_tag="ACHJ8C_001002"
     CDS             complement(19211..20704)
                     /gene="yidJ"
                     /locus_tag="ACHJ8C_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5550899.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARADEPFLMVISYDEPHHPFTCPVEYLEKYTDFYYELGEKA
                     EDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVLVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIHFADVRSKMHDALLDYMDKIRDPFRSYQWNLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(20701..22416)
                     /gene="yidK"
                     /locus_tag="ACHJ8C_001003"
     CDS             complement(20701..22416)
                     /gene="yidK"
                     /locus_tag="ACHJ8C_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO5550900.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQLSDG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVIV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAIISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            22583..23449
                     /gene="yidL"
                     /locus_tag="ACHJ8C_001004"
     CDS             22583..23449
                     /gene="yidL"
                     /locus_tag="ACHJ8C_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO5550901.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNDEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTSTSMMDIPVGQQSVIYNGEVYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMHTVGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDR"
     gene            complement(23539..25200)
                     /gene="yidE"
                     /locus_tag="ACHJ8C_001005"
     CDS             complement(23539..25200)
                     /gene="yidE"
                     /locus_tag="ACHJ8C_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO5550902.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGVGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMAMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESTRTNGGALIRTINIRVENPNLHN
                     LAIKDVPILNGDKVICSRLKREETLKVPSPETVIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVARVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASSD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFIHTLVDGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(25396..25824)
                     /gene="ibpB"
                     /locus_tag="ACHJ8C_001006"
     CDS             complement(25396..25824)
                     /gene="ibpB"
                     /locus_tag="ACHJ8C_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO5550903.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(25936..26349)
                     /gene="ibpA"
                     /locus_tag="ACHJ8C_001007"
     CDS             complement(25936..26349)
                     /gene="ibpA"
                     /locus_tag="ACHJ8C_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO5550904.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(26343..26415)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            26654..26986
                     /gene="yidQ"
                     /locus_tag="ACHJ8C_001008"
     CDS             26654..26986
                     /gene="yidQ"
                     /locus_tag="ACHJ8C_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO5550905.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVLDTILLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(26988..28202)
                     /gene="yidR"
                     /locus_tag="ACHJ8C_001009"
     CDS             complement(26988..28202)
                     /gene="yidR"
                     /locus_tag="ACHJ8C_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO5550906.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAYVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIVE
                     GSKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGEKVSFTYNDHVMHQLDSALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLRGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EDGQLWITETDR"
     gene            28303..29403
                     /gene="cbrA"
                     /locus_tag="ACHJ8C_001010"
     CDS             28303..29403
                     /gene="cbrA"
                     /locus_tag="ACHJ8C_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO5550907.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYINITRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQARFAALKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSMM"
     gene            complement(29364..30701)
                     /gene="dgoT"
                     /locus_tag="ACHJ8C_001011"
     CDS             complement(29364..30701)
                     /gene="dgoT"
                     /locus_tag="ACHJ8C_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5550908.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAQMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFIGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYIMLVGDVKRVG"
     gene            complement(30776..31924)
                     /gene="dgoD"
                     /locus_tag="ACHJ8C_001012"
     CDS             complement(30776..31924)
                     /gene="dgoD"
                     /locus_tag="ACHJ8C_001012"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFO5550909.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELSDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(31921..32538)
                     /gene="dgoA"
                     /locus_tag="ACHJ8C_001013"
     CDS             complement(31921..32538)
                     /gene="dgoA"
                     /locus_tag="ACHJ8C_001013"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO5550910.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(32522..33400)
                     /gene="dgoK"
                     /locus_tag="ACHJ8C_001014"
     CDS             complement(32522..33400)
                     /gene="dgoK"
                     /locus_tag="ACHJ8C_001014"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO5550911.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENAADAFAAGLERGLNAPDILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQQVITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(33397..34086)
                     /gene="dgoR"
                     /locus_tag="ACHJ8C_001015"
     CDS             complement(33397..34086)
                     /gene="dgoR"
                     /locus_tag="ACHJ8C_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFO5550912.1"
                     /translation="MTLNKTDRIVITLGRQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            34365..35021
                     /locus_tag="ACHJ8C_001016"
     CDS             34365..35021
                     /locus_tag="ACHJ8C_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010377884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5550913.1"
                     /translation="MKLNLKGFFKAASLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDEVVNMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPHGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(35065..35877)
                     /gene="yidA"
                     /locus_tag="ACHJ8C_001017"
     CDS             complement(35065..35877)
                     /gene="yidA"
                     /locus_tag="ACHJ8C_001017"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO5550914.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDSNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(35992..36390)
                     /gene="yidB"
                     /locus_tag="ACHJ8C_001018"
     CDS             complement(35992..36390)
                     /gene="yidB"
                     /locus_tag="ACHJ8C_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO5550915.1"
                     /translation="MGLFDDVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQSNQSVSGEQVESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            36508..36609
                     /locus_tag="ACHJ8C_001019"
                     /pseudo
     CDS             36508..36609
                     /locus_tag="ACHJ8C_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36630..39044)
                     /gene="gyrB"
                     /locus_tag="ACHJ8C_001020"
     CDS             complement(36630..39044)
                     /gene="gyrB"
                     /locus_tag="ACHJ8C_001020"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO5550916.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYDILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNAMQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(39073..40146)
                     /gene="recF"
                     /locus_tag="ACHJ8C_001021"
     CDS             complement(39073..40146)
                     /gene="recF"
                     /locus_tag="ACHJ8C_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO5550917.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(40146..41246)
                     /gene="dnaN"
                     /locus_tag="ACHJ8C_001022"
     CDS             complement(40146..41246)
                     /gene="dnaN"
                     /locus_tag="ACHJ8C_001022"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO5550918.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(41251..42654)
                     /gene="dnaA"
                     /locus_tag="ACHJ8C_001023"
     CDS             complement(41251..42654)
                     /gene="dnaA"
                     /locus_tag="ACHJ8C_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO5550919.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            42951..43031
                     /locus_tag="ACHJ8C_001024"
     CDS             42951..43031
                     /locus_tag="ACHJ8C_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5550920.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTRQPV"
     gene            43261..43401
                     /gene="rpmH"
                     /locus_tag="ACHJ8C_001025"
     CDS             43261..43401
                     /gene="rpmH"
                     /locus_tag="ACHJ8C_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO5550921.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            43418..43777
                     /gene="rnpA"
                     /locus_tag="ACHJ8C_001026"
     CDS             43418..43777
                     /gene="rnpA"
                     /locus_tag="ACHJ8C_001026"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO5550922.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            43741..43998
                     /gene="yidD"
                     /locus_tag="ACHJ8C_001027"
     CDS             43741..43998
                     /gene="yidD"
                     /locus_tag="ACHJ8C_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO5550923.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            44001..45647
                     /gene="yidC"
                     /locus_tag="ACHJ8C_001028"
     CDS             44001..45647
                     /gene="yidC"
                     /locus_tag="ACHJ8C_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO5550924.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            45753..47117
                     /gene="mnmE"
                     /locus_tag="ACHJ8C_001029"
     CDS             45753..47117
                     /gene="mnmE"
                     /locus_tag="ACHJ8C_001029"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO5550925.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11706 a        12688 c        11862 g        10932 t
ORIGIN      
        1 atgcagaata gagtgttcac accacatctg gtatagaaaa gcacaggcta gcaaaaacct
       61 gaataacccc gtacttacgg agttattcag gttaaaaata tccataacat cacttctgat
      121 ttttattgct tacgacggat ataatttctc tggtcagcta ctaatatcat catgctcacg
      181 cgtttaatca aactcgtgtt tctcaaacgg acatcgcggg acaatcttct gcttctcccg
      241 gcgcattttt accaccttcg ctacctgcat tttgagcgga agatccgctt ttttctggcc
      301 cccgcacata tgcaagtcca gttttaaatt tatcctttat ttcattctga aacaaactgc
      361 tcataatttt ctccagtgga acctgcatct cagggagaca tcactacaga atacttcccc
      421 ttatgcactg atttaatttt ggtcatttag aataacggcc tgtaaaggct caatgcaaca
      481 gatgggtaac gctattatga agatcctcgt cagtaaatta ctggcaaggc actccgataa
      541 ataagaatag gttccgcaac aacgttatat gcaaatacct cacgcttcgg ctagattatc
      601 gccggctctg ttgacgaaat tatggttgaa atctggagct accacacgtt gttctggctg
      661 gcgataggga tgttgggtat tgcaatgatt tgcttgctgt ttattaaaga tatttagctc
      721 accagtctgg gctaagaaaa atcggcgtgg cgcaaactga gccatcctcg ccatttttgc
      781 tatcatgcct gcatacataa acgacaaaac agtatgcaga gggaaaaatg ggttccacca
      841 gaaaggggat gctgaacgtt ctgattgccg ccgtgttgtg gggaagttca ggggtctgcg
      901 cgcaatacat catggagcaa agccagatgt cgtcgcagtt tttgactatg acgcgtttga
      961 tattcgccgg tttgattctg ctgacgctat catttgttca tggtgataaa atcttttcta
     1021 ttattaacaa tcacaaagat gccattagcc tactgatttt ttccgtggtt ggcgcgctaa
     1081 ccgtacagct cacttttttg ctaaccatcg aaaaatcgaa cgcagccacg gcaacggtgc
     1141 tgcaattcct ctcaccgact attatcgtcg cctggttctc actggtgcgt aaatcccgcc
     1201 cgggcattct ggttttctgc gctattttga catctctgat cgggactttt ttattggtga
     1261 cacacggtaa tccgacgtca ttatcgatct ctcctgccgc gttattctgg ggcattgcct
     1321 cggcatttgc tgctgcattc tataccacct atccctcaat gctgattgcc cgctatggca
     1381 cgttaccggt cgtcggctgg agtatgctca ttggcggtct gatcttgctt cccttttatg
     1441 ccggacaagg gacaaatttt gtcgttaacg gaagtttgat tctggcgttt ttttatttgg
     1501 tggtcattgg tacgtccctg acatttagtc tgtacctaaa aggagcacaa ttaattggcg
     1561 gtccaaaagc cagcatttta agctgtgctg aaccattaag tagcgcgctg ctctctttgc
     1621 tgttgctggg gatcaccttc acattgccgg actggctggg aacgctgctg attctgtcat
     1681 cggtgatttt gatttcaatg gattcccgcc gccgcgccag aaaaataaat cgtccggcgc
     1741 ggcatgagtg agaattacca gccaggcacc gcgccaccgt taaagatggt ttctgccgct
     1801 ttagcgactt cgggtgattg ataagattgc agaaattcct tcacgttttc tgcattctta
     1861 ttatcttccc gtgccaccag aatattcaca tacggcgaat ttttatcttc aataaatacg
     1921 ctgtcgttaa ccggagaaag tcctgtttgc tggatgtaag tggtgctgat aatcgctaca
     1981 tccactttcg ggtcgtccag tacacgtggc aactgtgccc cttccagctc cattatctgc
     2041 aaatggcgtg gattatcggt tatatccaac gcggtaggta ataacccttt tccctctttc
     2101 agagtaatta gcttctcttt ttgcagcagt aaaagcgcgc gaccaaggtt agtcgggtcg
     2161 ttggggatcg ccactgtcgc accttctttt atttgcgcca cggttttgat ttttttggaa
     2221 tagcccgcca tggggaagac aaacgtattt cccaccgcta ccagtttata accatgcgtc
     2281 tgattatctt gttcaagaaa gggtcgatgc tggaagacgt tcgcatcaag ctcgccatga
     2341 tttgtcgcat cgttcggtag caacgaaccg ctaaatccta ccagttcaac atcgaggcca
     2401 tatttctctt ttgctacctt tttagcgact tccgcgacat cttgttctgc gccattaata
     2461 acgccaactt taatatgctt ttcatcgctg ctgctctggt cgcaacctgc cagcagaact
     2521 cctgccagta ataatgcggc ccccgcccgt agatgatgtg ttgtcagttt cattttttta
     2581 tccttttatt aatgattacc ggactatagc ggtaaggtcg ctgcagttta aaaaacaaaa
     2641 cgctatcgat aagaataaaa aggaataaaa gtggaatata aagtctggca cttcttactg
     2701 accacgcaaa cacctttcgt acagcatgat gagagcggca aaagcaaact acacttatgc
     2761 ttcattcgat gcacatttgc agaaggttgc cgctatgaag ccaacgatgc tactcatgat
     2821 taccgtattc ctgatttttc cagccatttc tcaggccgag tcgccattca gttctctaca
     2881 gtcggcaaaa gagaaaacca cggtattgca agatttacgt aaaatttgca cgccacaggc
     2941 gtcattatcc gatgaagcgt gggaaaagtt aatgttgtca gatgaaaaca ataaacaaca
     3001 tatacgcgag gccatcgtgg cgatggaacg taataatcag agcaattatt gggaagcgtt
     3061 gggtaaggta gaatgcccgg atatgtaaga acggcaacaa ccactgacca acgcttcttt
     3121 acctgatatc aggatttctt cattttcacc tttgcagtta ccaacaatgc agtcagcaac
     3181 atcaatgtgc cggacaacat cagcggcgaa gtcagaccaa tgttatccaa cgcataaccg
     3241 ccgattgccg caccgcaggt attggcaagc tgaataacgg ccacctgaat agatccggct
     3301 ttttctgcct gatcggccag tgagcgagtg atccacgttg accagccaac gggaattaaa
     3361 gcaaaggtca gcccccagat aatcgccaca ccagtagcaa caattttatc gcttccccac
     3421 agcgtcagta ccagcgcgct cagggcgagc acaaacggtg cgcccgctaa ggccagtttc
     3481 accgaacgtt tgagaataaa cgacgaaagc gatgtcccga caaagctggc gataccaaaa
     3541 ctcagcagca ctagggttaa gccatcaaca ccgaatcccg ccagattcat atacactggg
     3601 cgaatataag tgaaaaaagc aaactgtccg gcaaaggaca taaagatggc gatcatcccg
     3661 gccatcactc ccggacgttg taataagcgg aaagtatttt gtttctgatg cgaaggttca
     3721 cctggcagcg aaggcagtga tttgataatc cagaaaatac acagcacgcc catcgccgcc
     3781 gccgcattaa agacattgcg ccaaccgata agctcgccta aaaaactgcc caacggcgca
     3841 gcaatcacca gcgcaataga aaccgcgccg aagatcaccg acagcgcctt cggcaccgta
     3901 cgcggcggta ccagacgcat ggtcagcgac gccgacatcg cccagaaccc gcccagcgcc
     3961 agccccagac aggcgcgacc gattaacagc agactgaatg agttggcaaa ggaaaccagc
     4021 aaacaggaaa gcgtcagcaa aacggcgaac agaatgacaa cgtagcggcg gtcagtggcc
     4081 tgaatggtct gggtaataaa caaactggca aacatcgcca caaaggcggt cacggtcacc
     4141 gattggccgg caaccccttc cgaaatgcct aaatcctggg ccattggcgt caacaaactg
     4201 acaggcaaaa actcaacgat aatcagacag gcgacgcaaa acgccaccga gaaaacggct
     4261 gaccagttcg gtcgggtgat ggcattcgcg ccgcggtttt cagcaataaa ttcactcatg
     4321 gtgttacccg tggcatgttt cagagggaaa gccgaccact ttaacattga aatgtgtgac
     4381 gtatttaacg tttttgcaac ttttacggtg ttgtgggaaa agttcgaggc tgctcgaaga
     4441 aataaaaacc atagggcaag gtgatatgca ttttcgcgat taggttggga ataagggata
     4501 aagtattgca cttggtgata tactttaata acaggatctc aacgatatgc gcgtgtttaa
     4561 aaccaaatgg tttgcaaggg aagccaggtc tcatgcaata acagatgaag agttgtgccg
     4621 ggccatttta gaaacggaac aaggtaaagc tgatgtactt ggtggtggcg tttttaaaaa
     4681 gcgccttcac aaaaatcgtg agcgtgcaat tattctggcg aaagggggaa gcaattggtt
     4741 ttacacgttt ttatatgcaa aacaggatat gtcgaacatt aatagtcagg aactggctgg
     4801 gtttcgtgaa cttgcaaagc attatgcctt cctgacgaaa gcccaactga cagcaatgat
     4861 taacactaaa gaactgacag agatctgcta tgactgcaaa aactaaattt aaaagtcctg
     4921 catttgaagc tattcacagc gcagcggctg gattgtccag cgtcgatgct atttctgccg
     4981 aaacgatgcg tacttttgac aaagcctgcc tgaccagcgt gcaagactta caacctgttg
     5041 agataaaagc actgcgggaa aaattaaaag tgagtcagcc cgtcttcgct cactatctca
     5101 ataccagcgt ttcaactgta caaaaatggg aaagtggcgc taaacgcccc aatggcattt
     5161 cattaaaact gctcagcatt gtgcaaaaac acgggctgga ggtgctgctg taaatagaac
     5221 gtagataatg cgttcaccta ctgtcagttc cgataccgga tgcgacgtac acgtcttatc
     5281 cacccaccag gtcaggcgcg gtctgtaggc tgataagacg aagcggtttt aatgcagcca
     5341 gtgaacgccc tcggcaggct gaaagaacgc gttatagcgc atctgaaaag cattgatatc
     5401 ctgcatttca ggttcggttt cgcgcagaaa acccagcgcc agcctgacct gagcatcggt
     5461 aatcggcgcg gcaaggcggg ctttttgtaa aatccgatgc cattgcagca ggttctgttc
     5521 gctaccatcg acaatgacaa tttgccagat gagcaacacc tgcgcggcgt tttgcaggtg
     5581 cgactcatcc gggcgatgca aataacgtaa aaattcgtca ccggctgact ttcccatttg
     5641 atcgaccatc gactccacgg gtacgacatc gatgttcagc cgatcgctga gacggcgaat
     5701 cgcttttctg gccccggatg tcagcacccg aaaaccaacc acaaacacta ccgccagcgt
     5761 ggcgagcatt atccagatca tgagttctcc tgcgtaatca gaactcatga tatctggaaa
     5821 ccctcgccag ttactaatcc accagaataa tcttcagtgc aaacagagtt gcgaccacca
     5881 ccacacacag gttcagatcg cgccagcgcc cggtgcacac tttcatgatg cagtacgaca
     5941 taaagcctag tgcaatccct tcggtgatcg agaaagtaaa gggcatcatc accgtggtaa
     6001 taaacgcagg caccgattcg gtaaaatcat cccagttaac gcgcgccagg cttgaagtca
     6061 tcagcacgcc aacaaagatt aacgctccgg cggttgcgta aggaggaacc atcgccacca
     6121 gcggtgagaa gaacataact aacaggaaca taacgccgac tacgacagcc gtcagacccg
     6181 tgcgaccacc gacagccaca cctgaagtac tttcaatata ggcggtaaca gacgaggtac
     6241 cgataaacgc acccgccacc gaactgacgc tatcaacaca cagcgcctta ttcatattgg
     6301 ggaatttacc gttactatct attaagccag ctttatcagt cacaccaatt aatgtccctg
     6361 atgaatcaaa taagttgatc agcataaagg agaaaatgat accggcgagt tcaagtgata
     6421 acgcaccgct caaatccact tcaccaatga cgccgctaat atcaggcgga atggaataga
     6481 cgccgctaaa atgaacatca ccgaaaaata atccacagca agacgtcacc acaatagaaa
     6541 caagcaccgc ggcatgaaaa tgacgtgatg acaacacagt tataataaaa aaccctaaaa
     6601 tacctaacaa cacgccgtga gaacttaaat cgccaatcat caccagggtg tctttattgg
     6661 cgacaataac gccagtattt tttaatccca ttaaggcgat aaataatccg attccgctgg
     6721 tgataccaat acgtaaactt aatggaatgt tggagatcat ccagtaccgg atacgaaaca
     6781 gcgtgagcaa aaatagccca actgcgcccc agaatatcgc gcccatcccg gtctgccagg
     6841 agatacccat cgcccccacg accacgaagg caaagaaggc gttcaggccc attgccggag
     6901 ccagtgccac gggtaagtta gcaaataccc ccatcgcaat actgccgata ccggcaatca
     6961 aacaggtggt aacaaacacc acttttgggt ccatttgtgc cgcgccgagg atttgcgggt
     7021 tcacaaaaac gatgtacacc atggttaaaa aagtggtcat ccccgcaatc aattctgtgc
     7081 ggacggtggt cccatgctga ggtagtttaa ataatcgcga aagcgtccct gattcattac
     7141 tcacgtaatc ggtattgtca ttattcattt ttttgtccat tggaggagat ttaatccctt
     7201 gcttttgtaa aacaaatgac aacaacggtt cagtgataat tatcacattt caattgcaca
     7261 ttaatggata ttctttaata atctcgcgac gtttctttat gataaataat aatcaaattg
     7321 ataaaatcaa aatgagaaaa atatgaataa ttctattaac cataaatttc atcacattag
     7381 ccggactgaa taccaggaat tgttagccgt ttcccgtgga gacgctgttg ccgattatat
     7441 tattgataat gtctctattc tcgacctgat caatggcgga gaaatttccg gcccaattgt
     7501 gattaaagga cgttacattg ccggtgttgg cgcagaatac gctgatgctc cggctttgca
     7561 gcggattgat gcccgcggcg caacggcggt gccagggttt attgatgctc acctgcatat
     7621 tgaatccagc atgatgacgc cagtcacttt tgaaaccgcg accctgccgc gcggcctgac
     7681 gaccgttatt tgcgaccctc atgaaatcgt caacgtgatg ggcgaagccg gattcgcctg
     7741 gtttgcccgc tgtgccgaac aggcaaggca aaaccagtac ttacaggtca gctcttgcgt
     7801 acccgcactg gaaggctgcg atgttaatgg tgccagtttt acccttgaac agatgctcgc
     7861 ctggcgggac catccgcagg ttaccggcct tgcagaaatg atggactacc ctggcgtaat
     7921 tagcgggcag aatgcgctgc tcgataaact ggacgcattt cgccacctga cgctggacgg
     7981 tcactgcccg ggtttgggtg gtaaagaact taacgcctat attgctgcgg gtattgaaaa
     8041 ctgccacgaa agttatcagt tggaagaagg acgccggaaa ttgcaactcg gcatgtcatt
     8101 gatgatccgt gaaggatccg cagctcgcaa tctcaacgcg ctggcaccgt tgatcaacga
     8161 atttaacagc ccgcaatgca tgctctgtac cgatgaccgt aacccgtggg agatcgccca
     8221 tgaaggacac atcgatgcct taattcgccg cctgatcgaa caacacaatg taccgctgca
     8281 tgtggcatat cgcgtagcca gctggtcgac ggcgcgccat tttggtctga atcacctcgg
     8341 cttactggca cccggcaagc aggccgatat cgtcctgttg agcgatgcgc gtaaggtcac
     8401 ggtgcagcag gtactggtga aaggcgagcc gatcgatgca caaaccttac aggcggaaga
     8461 gtcggcgaga ctggcacaat ccgccccgcc atatggcaat accattgatc gccaaccagt
     8521 ttccgccagc gactttgccc tgcaatttac ccccggaaaa cgctatcgcg ttattgaggt
     8581 aatccataac gaactgatca cgcactcccg ctccagcgtc tacagcgaaa acggttttga
     8641 tcgcgatgat gtgtgcttta ttgccgttct tgagcgttac ggacaacgac tggctccggc
     8701 ctgtggtttg ctcggcggct ttggcctgaa tgaaggtgcg ctggcggcga cggtcagcca
     8761 tgacagccat aatattgtgg tgatcggtcg tagcgcagaa gagatggcgc tggcggtcaa
     8821 tcaggtgatt caggatggcg gcgggctgtg cgtggtccgt aacggccagg tacaaagtca
     8881 tctaccgttg cctattgccg ggctaatgag caccgacacg gcgcagtcac tggcggagca
     8941 aattgacgcc ctgaaagccg ccgcccgtga atgcggtccg ttaccggatg agccgtttat
     9001 tcagatggcg tttctttccc tgccagtgat ccccgcgctg aaactaacca gtcaggggct
     9061 gtttgatggc gagaagtttg ccttcactac gctggaattc acggaataat aaaaaaaagc
     9121 ccggcgtcat gccgggcaaa agtcaccagt tacgtttatg ccactgtcaa ctgctgaatt
     9181 tttttctcgc ggcggatttt gcgttcttcc atcaccgcta ctatcgccat caggcagata
     9241 caaccaatag cggcgatatc cagcgcggcg aaggtgcctg cccagccggt aaggccaaat
     9301 accggcgtcc catcggcaat cattcccaga cctaacttgg caaagctgtc accaatcagg
     9361 taagcgaagg tgcctttaat accatcggca gcgccaatcg cttttttagg tacaaagcca
     9421 acagcagcca caccaatcaa caattgcggg ccaaagacca ggaaacccaa cgcaaagaga
     9481 gaagccaggt agatatattc gttgctggcg tgttgataca caccgagcgt ggcgataatc
     9541 agcgccagcg cgatacaggc caccaggcca cggcgaccgt tcgccaggtc agagagccag
     9601 ccccacagca gcgtaccgac cagcgcacca gcttcaaaca gcgtaaagcc ctgaatcgcc
     9661 accgctttag agagtttcag ttcctggaac gcatacacgg ttgaccactg gtcgatacca
     9721 atacgcacca catagaggaa aatgttggca aagcacagca gccagatcac tttgtttttc
     9781 agcacatact caacaaagat ctgccacttg gtcatatcgg tagattctgt ctctttatcc
     9841 tcttcactga tctcctcgcc aaacagctct tcagctttac cgaggccata agattccggg
     9901 gagtcgctgc cgtaacgcag accgataaaa ccaacaatca gcgcgataat cgacgggaaa
     9961 ataaacatgc cgatgacatg gccatcgaac aggtaatttg cgccgaacag cgccacacct
    10021 gctgcgcctg caccaccaag gttgtgagaa atgttccaga aaccgaggaa tgtcccgcgt
    10081 ttacgacgcg gcgtccattt ggtgatggtg gagtaactgc acgaaccgcc ggtactctgg
    10141 aaaaagccgc tcaaggcgta gaaggcaatc atcaggaaca ggctaaccga gccgctgccc
    10201 atactggcac tgaagcccag catacaaatg gcggacagga tcagcatgaa cggcaggaat
    10261 tgtttggtgt ttttgccgtc ggcgtagtaa gaaaccagcg ttttacccac gccataagtg
    10321 atggagaagc ccaggccgat catccccagc tgcgtcatgc tcaacccgta ggtcgagatc
    10381 atgtcgttct gcgcgatgtt aaagttcttg cgaatcaggt acatcgtaag gtagccgata
    10441 aagaccacca ggtaggattg catgaacggt ttgaaccaca ttttgcgccg cacttcgagc
    10501 ggaaggtcca gggtcggctt gcgaacctgg tttaagaaag ccagcatggg ttactcctga
    10561 aatgaatacc tgcgtgaggc atgcattgta aaaatcagca gcaaatcagg cctgagatag
    10621 cgtccaggca aaacctggaa aatttcttag ttttgccgga tatgaagtga aaaggtgaga
    10681 tgcatcacgc ttctcgcggt gtctgggcgt tcaaaaaggg cagtaacagc agtgcggaaa
    10741 tcccggcggc gatggcgata accacaaaaa atccgctcca gtgccaggta tcgagtactt
    10801 tcgccagcgg ccaaccagca agcgacgcac caagataagc aaacaagccg acaaaccccg
    10861 tcgccgcccc tgccgcctct ttgtgggaac actctgccgc cgccatgccg attaacatct
    10921 gcgggccaaa gacaaaaaaa ccaatggtga agaagcaggt tgcctgcatc acgtagctgg
    10981 caaatggcat cagccacagc gagccgactg aaagcaaaat tccggcggcg aaaatcaaat
    11041 tcatcggccc gcggttgccg ttaaacagtt tgtccgaacc ccaacccgct accagcgcac
    11101 cgataaatcc gcccagttca aacattgtca ctgccgtatt cgccgtgacc agatcgacgc
    11161 ccagcgtctc ggacatatac aaattgcccc agtcgttgat cgccgcccga accacataga
    11221 ccagcacata gcaaaacgaa agcagccaga tatacggatt cagcaacaca tatttggtga
    11281 ggatctcttt acgcgtcagc cctgcccctt cttgttgttg agcaatctcc agtgcgtcgt
    11341 gccgccattc accaaccgcc ggtaaaccta acgcctgcgg gcgatcgcgc agccgccaac
    11401 agagaaaaat ccccacgact atcgccatac aacccgcaat catcatcccg gcacgccagc
    11461 cgtaatgcag cgcgctcgct gccatcacaa tgggaatgag tgcgccgccg acgttatgtg
    11521 ccgtgttcca taatgcccac caaccgccgc gctcggtacg agaataccag gcggttaaca
    11581 gacgcgcaca caccggtgaa ccccagccct ggaaaaaggc gttcaacacc caaagcacgg
    11641 caaacgccca tagcgacgtc gagaagccaa acagaatgtt gataatgccc gtggcaataa
    11701 gccctatccc cataaaataa cgggcatttg agcgatcgct gacaatgccg gagacaaact
    11761 tcgacacgcc gtaggtaatg taaaacaggg tcgctaacag gccaatatcg ctacggctga
    11821 gcacgccgtt agcaaggatt tctggtacgg cggcgttaaa acttttacgc gtgaagtaaa
    11881 acagcgcgta acccagccag atggtcagca gaatatgccg acgccagtag cgatagcggg
    11941 catcaatttc atgtttatca gtcattaatg gcgcatcggc gggcgctttc agaaacggca
    12001 acatcatcga accttagaca taacgttgag gtagagaaac gctgacacgc gtgccgtgca
    12061 ggcaggagat ggtcaatgtg ccacccagcg ccgttacgcg ctcgcgcatt ccggtaaggc
    12121 caaaaccgtg ttgcccggaa tctggcggta aaccgctgcc atcgtcttca atcaccagca
    12181 ttaaccgctc atcatgctgc cagccttgca gcgtgaccgc actggcatcg gcatgtttca
    12241 caatgttgtt caacccttcc tggcagacgc gaaacagcgt cacgcgctgg ttttcgctta
    12301 acgctgattc atcgattcgc cattcgagat gactgacaat gccgcgccct tccagctcca
    12361 tttcccgcat cagtgagcgg atggcttgct ccagagtgag atcatccagc tggcgcggac
    12421 gtaaccgccc taacaagcgg cgcaccgcgt cgtaaacgcc caacgacaac tgttcgatga
    12481 gctgcccgct ctgtttcacg ctggcgttat ccgccgcaag ccgctggaca atgcccgctt
    12541 gagttcgaat agcagtaatg gtctgaccga tatcgtcgtg cagctctcgc gccacatcgc
    12601 ggcgcacgct ctcttcagtt tctaacaaac gttcagccag atgctggttg cgcgccagtt
    12661 ccttttgcag tgactggtta agttcacgca accgctggat gcctgcgccc agtaacaacc
    12721 ctgtcagact ttgcaccagc agcgagagca ataaatccac cggatgatcg cgccaggttt
    12781 gactggcgat cagcgcgatg gcgttcatca atgtcgcaat cagcgctcct tgccaaccat
    12841 agtgccaggc cagcgcgata atcggcagcg ccagacagaa tggcgtaaag cgcgacagtt
    12901 cgtctggcaa tcccaactgg agccagagac tgataacaaa cagcagcaag taccagacca
    12961 gatgtcgccc gcgccagttg attggttgag aaaccagtga cgggccgagc ggcagccagg
    13021 tgttattggc gagatagtgc cagaacacca gacatatcgg ggccagcgtc aggccgccag
    13081 ttaaagtcag cagcagcgca ttccacgact ctttgccgtg ccaaagccag ggcagcgact
    13141 gcaacaacgc cgccgccgtt aacgccgcac cctgtaacag caaggtgcgc cagtcacgtt
    13201 gatggcgata gcgcgagatc agcgccacgg gcaacaacgt cagtaaacta ccgatcatca
    13261 ataaggagaa atgggttaaa ccgaccgcct gcattagcca gtaaatcagc agccactccg
    13321 cacccagtag caccggccag tatccgcgcg ggcattgcag cattagccct agccgcaggc
    13381 caaacggaaa taacagcacc gccatatcag ggcgctcaac caggtgcagg ctgatactcc
    13441 acaggcaaaa ccatgcggca gagaagataa aaaagcaggc aataacggta attaagcggg
    13501 agaacaacgt cttcatcacc agccatcaaa catgcggcgc gccagttcaa cgtcgttact
    13561 gacgcccagt ttttccatca gattggcgcg atggacgtgt accgttttcg gcgacaagcc
    13621 cagttcggcg gcaatctctt tcaccgccat tccttgcgcc agtttttctg ccacctggcg
    13681 ttcgcgtttg gttagcgggt cctggcgacc ggatgccagt ttaatggcaa tatccggcgt
    13741 cagataacag ccgccagtgg caaccgtatg caccgcagca atgagttcat ccgggctaca
    13801 gcgtttggag agaaagccgc gcgccccggc gttaagcgcc tgctcaacca gcgccggact
    13861 gtcgtgaacg gagagcataa ttgtcgccat gcctttcggc agctggctta gaagctccag
    13921 accggagata tcaggcatgg agatatcgca aatacacacc tgcacaccgc gccccggcag
    13981 ccccgccagc gcctcgcgcc ccgaaccaaa ctcggcaact acctgcaaat caggttccag
    14041 ccccagcagc tgcgcaaagc cggagcggac gataaggtga tcgtctataa gggcaacggt
    14101 gatcatgatc ttgtcctggc gggtaataaa acgcgcttac cttaacgata agcgcgatgt
    14161 tgttcaagcc ttgagcggtt actgaaaaaa caccgcaatc ttgttaaaca tggtcggatc
    14221 ggactgatta cgctgcactt tcaccacatc ttccagcttg tcgatttggc ttatcatctg
    14281 ctccagacgc tggtcgtcat tgaccagtag ccagatgtgg cttttgtcgc tgtcctgaat
    14341 cggcaggcag agaatgcctt caacgttaaa agcgcgacgg gcaaaaaggc cacaaacgtg
    14401 ggtcattaca cccggatggt tgcgaacggt gagttccaga atcacgttat catgagttgt
    14461 gttttgcatg gcttattccc ccaccatttc agtattcgcc gcacctggcg gcaccatcgg
    14521 gtacactttt tcttcggcat caatgcgcac atggatcagc gccgggccag ggcgattgat
    14581 gatttcctgc aatgcagcct gcggatcggc ttcgttattc aaatcacagg tttcgaggcc
    14641 gaatccggcg gcaatctgca taaagttgat tttgcccgga taggtagcgg caaaaacacc
    14701 ctgcttgtag aacagactct gttgctgatg caccagcccc agcgcttcgt tgttcatcaa
    14761 aatgatttta acatccagct gattttcact ggcggtcgcc atctcctgaa tattcatcat
    14821 caggctgccg tcgccggaga agcacaacac tttgcggtcc gggttcgcca gcgccgcgcc
    14881 aatcgccgca ggcaggccaa aacccatggt gcccagcccg ccggaggtca gccactggcg
    14941 tgggcgattg agcggatagg cttgcgcggt ccacatctga tgctgaccca cgtcggtggt
    15001 gataatcgca ttgtcatcga cacaggcggc aacggcgttg atcaggccgt aatggcttaa
    15061 tggatcgcac gctttcggga ttggacacgg gaattcacgc tgcaaatccg ccaccaactg
    15121 gtgccactct gcacgcggtt gcgcttccac cagcgggatc aactgcgcca acacgtcatc
    15181 aacatccgcc tgaattgcca catgcggttg cttgattttg cccagctctg cgcggtcgat
    15241 atcgacatga ataattttgg cattcggaca gaactgctcg gtcttgccaa tcgcccggtc
    15301 atcaaaacgc gcaccgagta caatcagcag atccgcctcc tgcaaaatat agttggtgct
    15361 gcgcacaccg tgcatcccca gcatacccag cgacagcgga tgcgcttttg gcaacatacc
    15421 cagcgccatt aaagtcatgg tggtaggcag ttgtgctttc tccgccagtt cacgcactcg
    15481 cgcaggcgcg ttgatcacac caccacccag ataaagcacc gggcgtttgg cggcgttaat
    15541 catcgccgcc gcgtcacgaa tactttcttc gctaaaggcg ggtgcggcgg ctttttctgc
    15601 catagcgggc tgtgtttcaa tctcaaaaac tgccgtttgc acatccttag gaatgtctat
    15661 ccacaccggg ccagggcggc ctgattgcgc aatacggaag gcatcgctca tgacctgcgg
    15721 gagttcttcg atatgtctga ccagatagtt gtgtttggtg atggggatag agatgccgta
    15781 ggtgtcgact tcctggaagg cgtcggtgcc gatcatcgag gcggaaacct gaccggtgat
    15841 gcaaatcagc gggatggagt ccagcctcgc atcggcaatg gcggtcacca ggttagtcgc
    15901 acccggtcca ctacaggcca tacagaccgc cggtttaccg tcggtgcgcg ccattccctg
    15961 agcgataaac cccgcgccct gttcatggcg agccagaata tggcggattt gcgtgctttg
    16021 gcttaaggca tcgtaaacag gcaggataga accgcccgga atacccgtca caatcttaat
    16081 gccctgctgt tccaggaaat gaacgataaa ttctgcgccg gtaaagcgct tacgcgtcga
    16141 tgttgtgccc gaacttgcca tgctccagtc cttttcttct gggccgactt tccgggaggt
    16201 gcttaaacga aaaaccccgc ccggtttgcg ccggcggggt tttggaatcg tgtgttgttc
    16261 cagtccctac ggcgcattgc cgacgaccac caccacacgc acgacgacca ctgcggcgga
    16321 tggcgcagtt ggtagtagtt ttgcgttgaa catggaagta gtcattaggg accttgttgg
    16381 ttttgtgttt aacaatattt atacaagcac agctttacag gggagacaat ggaaaatttt
    16441 tcttccggat aaaaatacgt ggcagatcac attttaaact gaaactcagg caataaagca
    16501 tcatcacctg aaagacggtc agattgttga cagagtgcgc cttattcatg tcgaatacag
    16561 cgtaaacacc ttaccctacc aaccgcacca tcactgtttc cggcaacatt tattgccgct
    16621 tttgtttaca tattctgccg ctaaacaatt ccccattcct ggcgtatatc tggctaacat
    16681 tcatcaatgt gatagattcc tctcccgcat ttatgggaat gcgtagtgac ttattctaat
    16741 tatttttata aaagcatccg tgataatgaa aaggcataga aacgtcaatt tgttattgat
    16801 gttggtatta ctcgtggccg tcggtcagat ggcgcaaacc atttatattc cagctattgc
    16861 cgatatggcg cgcgatctca atgtccgtga aggggcggtg cagagcgtaa tgggcgctta
    16921 tctgctgact tacggtgtct cacagctgtt ttatggcccg atttccgacc gcgtgggtcg
    16981 ccgaccggtg atcctcgtcg gaatgtccat ttttatgctg gcaacgctgg tcgcggtcac
    17041 gacctccagt ttgacggtat tgattgccgc cagcgcgatg caggggatgg gcaccggcgt
    17101 tggcggcgta atggcgcgta ctttgccgcg cgatttatat gaacggacac agttgcgcca
    17161 tgctaacagc ctgttaaaca tggggattct tgtcagtccg ttgctcgcac cgctaatcgg
    17221 cggtctgctg gatacgatgt ggaactggcg tgcctgttat ctctttttgt tggttctttg
    17281 tgccggtgtg actttcagta tggcccgctg gatgccggaa acgcgtccgg tcgacgcacc
    17341 gcgcacgcgc ctgcttacca gttataaaac gcttttcggt aacagcggtt ttaactgtta
    17401 tttgctgatg ctgattggcg gtctggccgg gattgccgca tttgaagcct gctccggcgt
    17461 gctgatgggc gcggtgttag ggctgagcag tatgacggtc agtattttgt ttattctgcc
    17521 gattccggcg gcattttttg gcgcatggtt tgccggacgt cccaataagc gcttctcaac
    17581 gttgatgtgg cagtcggtta tctgttgcct gctggctggc ttgctgatgt ggatccccga
    17641 ctggtttggc gtgatgaatg tctggacgct gctcattccc gccgcgctgt tctttttcgg
    17701 tgccgggatg ctgtttccac tggctaccag cggcgcgatg gagccgttcc ccttcctggc
    17761 gggcaccgct ggcgcgctgg tcggcggtct gcaaaacatt ggttccggcg tgctggcgtc
    17821 gctctctgcg atgttgccgc aaaccggtca gggcagcctg ggattgttga tgaccttaat
    17881 gggattgttg atcgtgctgt gctggctacc gctggcgacg cggatgtcgc atcaggggca
    17941 gcctgtttaa gctcacgtca ccgcagcatc gtcatcagct ccatgggaga acgatgctgc
    18001 tttatcagat cacgcatcac ccgcatatgc ggcgcggagt aagaataaaa cgcctgatag
    18061 cccgcacaaa gcacgttgtc tggtttatct tcccgagcgt gcgggcacag ccgatgccag
    18121 tcgcacgcct ggcagtcacg gcgcggttgc gacttttcgc tgccacacca tttttgtaag
    18181 gtggtgtcaa aaagttcaat cgaaatacga ctgatatctg cacgcaccca acactcgaac
    18241 agggcaatta aaaatttccc ccacagctct gctgtcaccg aagcggccgt cagatcgccc
    18301 cggtcatcaa attcaagatg gggaataagc tgtactgagc ggatgcccag ctgtttgaaa
    18361 ttgtgataaa aatcggcagg ttgcagagca tccttatact ctacaaccag tttatgcctg
    18421 tcctcttcag catcaatgac ctgacttcct gtcatgcgac tctctcaata aatacgataa
    18481 gttgatgtat atgcgttaca gcaaacagta ttgcgagaga taacaccgcg agggagatca
    18541 aaaatttgtc acggactacg tgaaattggg aaaaatcgct attcgtgaca tccattaaat
    18601 tgcgattacg ggtgtagtgc cagaggatca gcgccacaat ggcgaggatg ccaatcgaaa
    18661 tccagaataa catgcccgcc tggtgccagt tgtgtttgat agccaacgcc atcaacgcgc
    18721 cgtagcccag catagtacga aaccacgcca gtgatgtgcg ttccggctgc agccctgggt
    18781 cggcaatgcg tctggctttg cggctatccg gcatacaaca ccagtcccat gacgatcact
    18841 gccacgacca tcaatattaa actgatgatg aataagcttt tggtataggg caggtcctct
    18901 ttcaggcgca tcgctttttc attgcgtaac cagcgcaagt agccatacat cgctaacccg
    18961 ccggaaaaca gacacaacaa caacgccagc agttcgcgga tgacgggcgt ggcgaaatct
    19021 ggcgcaagtt gatcaagccc aacccccgcc gccagaaatc ctagtgcagt acggatccac
    19081 gccagaaaag tacgctcatt agccagcaag aagcggtaat cgggcgcttc tccgaggcgg
    19141 gaaattttca ttgttgctcc ttgtaaactg ttcctcgccg aaaaattagg ccaggaacag
    19201 caataatgca tcagaacttc tgttttttct cttccacctt caccccttgt gtcggtaaac
    19261 cggtgtcata gtcgcgtacc actggagaat aaccatcctg tgggcgtgga cgaaacgccc
    19321 ccatccagcg cggttgtgca tctttgcgcc acggacgcag gttccattga taactgcgga
    19381 acggatcgcg aattttgtcc atgtaatcca ataaggcgtc atgcattttg ctgcgaacgt
    19441 cggcaaaatg gatatcatcg atcaggttat gcatttcatt tgggtcatta cggcgatcgt
    19501 aaagttcatc actggtgaag aggttgagca ccagtttaaa gtcatccgtt acccagcaac
    19561 gcaccggaat aaaaccgcca aagctgtcat gctcaatctc gtagcgatta aattccacca
    19621 acacgccacg tggctctttc acggcaagga tattttcccc cggcagaatc tctggttttt
    19681 caatatctgc cagcgccatc attgtcggca gtaagtcaat atgactgact ggcgtatcga
    19741 cctgtcgccg ctccccttgc ggcgaacgga tgatcagcgg aatgcgggtg atgtcgtcat
    19801 acatcgccgc tcctttactg atcagcttat gtgcgcccat catttcgccg tgatcggagg
    19861 tataaataac ccacgtattt tcacgttgct ctggcgttaa ggcgttgatg acccgtccga
    19921 tttggtcatc aacaaagtca ttgcaggcaa aatagagcgg atggtgataa agcccgtcat
    19981 caccgactgg cgatggcatc gcctgcgccc ataagcggtg atgttccggt ttgttcgcca
    20041 ggtcatcctc tgctttctca cccagttcgt agtaaaaatc agtgtatttc tctaaatact
    20101 ccaccggaca ggtgaacggg tgatgcggct catcatacga aatcaccatc aggaaaggtt
    20161 cgtcggcgcg cgcgggctgt tgcagaaaat ccaccgcccg attgctgatg cggtgcgccc
    20221 aggtgaaggt ttcgtcaata tggttcgcct gtaaatcctc aacgctgttt aggccattgc
    20281 gccacaggct aatctctttc tccgtcagtt cactaagata gttcgcgcca tcgaaccagt
    20341 aatcagcgtc ccactccggc ggacactcgc cagtgccgaa atagtcatgt ccatcgagat
    20401 gccatttgcc gatgtagcag gtgtgatagc ccgcatcctt aaagtagcgc cccatggtgg
    20461 aaatgttttt gcccggcgcg acgttgttgg tccacgggcc ggactggtta gcgtagatac
    20521 cggtaaacag cccggcgcgc gccggtgtac aaaccggtga acaggtgtag gcggaattaa
    20581 agcgaatacc ttctgccgcc agactatcaa tattttgcgt attcagcggt ttaccgctat
    20641 agcaaccgac catattggtg gcctgggtat cggtcatgat gaacagaaaa ttggggcgtt
    20701 tcataggcta tccttcgcat cgggagtaaa ggtgacggct gggtcgtgac gatgccgcca
    20761 gctgtcaaaa atcaggtaga caatcactac ggcggcgatg aaataactga ttatcgctaa
    20821 ccagcgcgta ccgtagccac cgaattcagc cagcccggca tagacgccaa tcatcgcgaa
    20881 caggatacca attgacgcaa tcttgacgtt tttccacggt ttcatgtcca ccgcaaacgc
    20941 atctttgaag gtgaacggcg tggcgcgcgg tttgataaaa ccgatcacca gcatcacgac
    21001 cacgttgatg cagaacgtac aggccagcac atagaggaaa tggaagtcga acttcaccag
    21061 atagttgatg gtgatgtagc tgattatgcc aatccccatc gccacttttg ccgccagcgc
    21121 cggaatgcgc gggaagaaaa agcccataat gatgatggta accagcggca cgttgtaaat
    21181 accgttgagc tgtttcatcc agctatacag cccctgcggc gcgttggcga tccacggcgc
    21241 gactatcacc gaaacgatgg cgataaagaa accaaatttg cgcccgacgg tgaccagttg
    21301 ttgcggctcg gcattctggt taatgatgcg ccggtaaata cccatactga ataacgtact
    21361 ggcgctattc agaaagccgt tgaaggtact gatcaccgca ccaaataaca ccgcaccgaa
    21421 gaagcccacc agtggcactg gcagaacgtt attgaccagc gtcgggtagg ccatgtcggc
    21481 tttcggtaaa tcctgataca gatgaaacgc aatcaaccct ggcagcacca gtaccagcgg
    21541 gtcgagcatt ttcagcaccg ccgttaacag cgcccccttt tgcccttccg ccaggctttt
    21601 tgacgccagc gtgcgttgca cgatgccctg atttgtacac cagtaaaagg tgttcaccag
    21661 aatcaaaccg gtaaatgccg cgccaatcgg caagggatcg gtcgggccac cgactgagtt
    21721 taatttctcg gcgtgaacgg tggtgagttg ctcaatgccc tgcataaagc tgcccttgcc
    21781 catcgcgatc aggccaaata ccggcaccat caacccgcca ataaccagcc caataccgtt
    21841 aatggagtcc gccactgcca ttgcgcgcag tccgccgatc accgcataca aaattcccgc
    21901 cagaccaagc aaaataacca gtaaccagat agccgcacca tcggaaagtt gtagcgactc
    21961 acccacatga aacaggctgt tgagcgccaa cgcgccggaa tagagaacaa tcggcagaaa
    22021 gcagacgccg gtggcaatga ggaagcagaa gtcgatgata atacgcgtcg ttttatcata
    22081 acgttcctcc agaaaatcgg ggatggtggc aatgccgcgc ttcagatagc gcggtaaaaa
    22141 gattagcgcg aggaagatca gcgtcactgc agaggtgact tcccagccca tcaccgacat
    22201 gccgcttttg taggcctgcc cggaaaggcc gaccagttgt tccgtggaaa ggttggttag
    22261 cattaacgaa gcggcaataa ccggcgcttt tagtgaacgt ccggcaagaa aatagccttg
    22321 ttgcgatccg gtatctgttt tacggacctt ccaccaggtg atgaccgcta ccagcagcgt
    22381 aaaaccgaca aaactcaaga tttgtaacga attcatctca tagcccttat ttatgtttat
    22441 gtcccggacg catgaatata gggagaggta aaatgcgtcg ggtcaggcag tcagattctt
    22501 aaaatacaaa cgtcgtatcc ctgaacggat tgttttctgt taagttcagg ttgtgagcat
    22561 aattcgccgg agggatatta aaatgaatgg aaaattgcaa agttcggatg taaaaaacga
    22621 aactccgtac aatattccct tattgattaa cgaaaatgtg atctccagcg gaatttctct
    22681 gatatcgctc tggcatacct acgccgacga gcattatcgg gtgatctggc cgcgggacaa
    22741 gaagaaaccg cttatcgcca actcatgggt tgcggtgtat accgtacaag gatgtgggaa
    22801 aattctttta aagaatgacg aacaaataac tctgcatggc aactgtatta tattcttaaa
    22861 gccaatggat attcactctt atcactgtga aggtttggtc tgggaacagt actggatgga
    22921 atttacctcc accagtatga tggatattcc cgtcggtcag caaagcgtta tttataatgg
    22981 cgaagtttat aatcaggaac tcaccgaagt tgctgagtta attacttcac cagaagcaat
    23041 aaaaaataat ctggcagtcg cttttctgac gaaaattatt tatcagtgga tttgtcttat
    23101 gcacacagtc ggtaaaaaag atccacaacg gcggcaaatt gaaaaattaa ttgccacttt
    23161 acatgccagt ctacaacaac gctggagcgt agctgatatg gctgccacga tcccctgtag
    23221 cgaggcctgg ttgcgtcgtc tgtttttacg ctataccggc aagacgccga aagaatatta
    23281 cctcgatgcg cgtctggatc tggcgctatc gctattaaag caacaaggga actcggttgg
    23341 cgaagtcgct gatacgctca acttcttcga ctcctttcat ttcagcaaag cctttaagca
    23401 taaatttggt tatgcgccgt cagctgtgct gaagaatacg gacaggtaat aaccagaatg
    23461 gcgagtgtgt aaatgactcg atgatgttca gaaataccag gcggagtacc atttacggcg
    23521 actccaccgg gaaaagagtt aaccgatact ccagaagagc accgccagta attggggtgt
    23581 gataatgcgc aggaacatca ctaacggata gacagtggcg taagagagcg ccgccgcacc
    23641 gctggttgga tgaagattat tagcaaacgc cagcgccgga ggatcggtca tggaacctgc
    23701 cagcatcccg cacatggtca ggtaattcat tttggctaac atccgcgcca gaataccaac
    23761 agtaatcagc ggaacggcgg tgatcagggc accataacca atccagctta gcccttcgcc
    23821 atccaccagt gtatggataa aatcgccgcc ggatttcagg ccaaccaccg agagaaagag
    23881 cacaatgcct aactcgcgta gcgccaggtt agcgcttggt ggcataaacc agtacagctt
    23941 gccgatactg ccgatacgcc cgaggatcaa cgccataatc agcggaccgc ccgccagccc
    24001 gagtttcaac gccgccggga acccaggcac aaagacggga atagaaccta acaatacgcc
    24061 aagcccgata ccaataaaca ctggcagcat ctgaacctgt tgcagttttt gctgcgcatt
    24121 ccccagcaca ttggcaacgg catcaattgc ggacggacgc cccaccaggt tgaggatatc
    24181 gccgaactgc aggctgatat cgctacttgc aactaattca acaccagcac ggttcaggcg
    24241 cgagataaca acgtcatagc gttctttaaa gtgcaggtcg cgaatacgtt taccgagcac
    24301 gttttcattg gtgaccacca cacgcgcgac gcgcaaatca gtgcctttcg tagacagcga
    24361 ggtatcgacc tcctgaccaa tcaccagttg cgcattatgt aaatccgcag gctgccccac
    24421 cagatgcagc aaatcgccca gttgaataac ggtttctggc gaaggcactt ttagggtttc
    24481 ttctcgtttc aatcgtgagc aaataacttt atcgccattg agaatcggca catctttaat
    24541 ggctaaatta tgcaaattag gattctcaac tcggatgtta atcgtcctga ttagcgcacc
    24601 gccgttggtt cgcgtggact cgtgctgctg ggcctcagtc tcaacattga cgcggaaaat
    24661 aacccgcagc atccacatag tgaacaaaat gccgcaaatc ccaaatggat acgccatcgc
    24721 atagctcatc cccatttgat cgaccatcgc catcggtgtc cccaggtcgc gcaagatctg
    24781 ctgccctgcc cccagcgctg gcgtattggt gaccgcgccg gaaaaaatcc ccagcactac
    24841 cggcagtgga atatcaaaca gtttatgcag gatggcggta accagaccac cgatgatgac
    24901 gatcagaaca gcaaacaggt tgaggcgtaa tccggagacg cgcaatgagg caaagaagcc
    24961 cggccctacc tgaatcccga tggtataaac gaacaggatc aggccaaact cctgaataac
    25021 atgcagcata tcgctactta atgtcatccc cgcctgagaa acaaaatggc cgacgatgat
    25081 cccaccgaac agcacgccgc caatgcccaa tccaacaccg cgaaacttga cgttgccgat
    25141 aaacaacccg acgaccgcca ccaaagccag aatactgacc gttaatgcta tatcactcat
    25201 cgtccatttc tcttgaaata acattttaat tgctacggat tatggctgaa tcggtgaagc
    25261 agatttgcct tatagcgcac attatcacgg gaaagaacgt gccgaaatat cttaaacagg
    25321 cggaatttaa cgcattgtcg aagaaaaaag acaaaaaaag ccccaccaga atggcggggc
    25381 aaagagaata gctagttagc tatttaacgc gggacgttcg ctgatagcga tacgctgcgc
    25441 tgcgatgggt tcaggctcgt tacgaattaa atcaatatgc agtaaaccgt ttacgaaggt
    25501 tgcgccagag acttccatat tttcagccag cgtaaagctc aggctaaaag gctgattcat
    25561 aagcccttga tgcagccatt ttttctcttc ttttggctgc tctggcgtgc cttttacgct
    25621 caggcgcgta ccttccagtt gaatctctaa atcttcctga cggaaacctg ccagcgcaag
    25681 ggtaatgcgg tagtggttat cgtcgctttt ctcaatgttg tacggcggga agctctggct
    25741 ttcaccggcg ttttgcagtg cgttggccag tttgtcaaaa ccgatccatt gacgcatcag
    25801 tggggataaa tcgaagttac gcatagtcat ttctccttct aagaagcgag taagtacctg
    25861 caaatccgaa gattcgcata tgctccctga cggcgagcat ggagatgtca ggccgcgcca
    25921 ggcggcctta gggaattagt tgatttcgat gcggcgcggt tttttcgctt ccggaatcac
    25981 gcgttcgaga tcgatataca gcagaccatt caccaggtta gcaccacgaa catgaatgtt
    26041 ctcagctaac tggaatttac gttcaaagtt gcgttcagcg atgccctggt acagataggt
    26101 gcgctctttt tgttcgtcgg cgtgagcacc tttcaccacc agcagattat cctgggcggt
    26161 aatttccagt tcgctctcag caaaaccagc cacagcgata gcaatgcggt aatggttttc
    26221 gtctaccagt tcaacgttat acggagggta gccgccatta ctctggctct ggttgttttc
    26281 taagtggtta aacaaacggt caaatccaat agcagaacgg taaagcgggg ataaatcaaa
    26341 gttacgcata atcaatagct cctgaaatca gcgagaatct aagaccttcc acaatggaca
    26401 ggtcaggtag ccagaacacc catcaggcgc gtcctcatcg gctacgatgt aaaaatgggt
    26461 ctggaaatga acttttcaag ccttatcagc ggactttttt tgcagtttat ggtctattgc
    26521 atagactgag ggggcagcac gaaacgttaa agtgcgatag ccgccacagt gcgacgtaat
    26581 gatgatgtta ttttcagcaa gggatcgcta taactcatca ggccaacacc cacgatcaac
    26641 agatgaattt attatgataa gaaatgtgtt gttagcgttc atgatatgca gcggaatgac
    26701 attactcgga ggatgctcca gcgtgatgtc ccataccggg gggaaagaag gaacgtatcc
    26761 tggcacccgc gccagcgcaa ccatgattgg cgacgatgaa accaactggg gcacaaaatc
    26821 gctggcgata ctcgacatgc catttaccgc agtactggac acaatattgc taccgtggga
    26881 tgtgttccgc aaagacagct cagtgcgctc gcgcgttgaa aaaagcgaag ccaacgcaca
    26941 agccaccaat gcggttattc caccggccag aatgcctgat aactgattta gcgatcagtt
    27001 tcggtgatcc acaactgacc gtcctccatc cacgccagcc actgaccatc cggcgagaag
    27061 accacggcgt ccgcagaagg tggattggcg tggttttcgg ttaaatactc aacctcgccg
    27121 ctttgcgcat gggcgcaagc aattcgatta tccagcacaa agcccaacca ttctcctgac
    27181 ggatgccagt taaatgcaga ctgaatatcc gttttgttat gggttaactg gcgcggctcg
    27241 ccgccctgtg gcgagataag ccacagttgc acaatgccgt tatcatcacg cattaaaaac
    27301 gcgatttgcg taccctgtgg attacagcgc acccagtggc gcgggacgtt gactaacccc
    27361 ggataagccc gatggtgggt aaaggttaaa cgtcgctgca cgacgccacg cggtggcgcg
    27421 ggcagggttg tttccgttcc tcttaacggc gcatcacctg ccgctttcca gccagcttca
    27481 tcttgcggta actcaacgat aaatagctcc ggcactttct cgccctttgg cgaaagtgtg
    27541 tcgccaataa acgccagcgc gtgatttcct acccatcctt cttcataagc acgattgatt
    27601 tcatcgctgc caggctgcgg cgtgggcgtg gttttactca ccagcacgca ccagtggcta
    27661 ccgctgtatt cacgcggatg ctgcttttgt acgttgaccg ggccaaacgg tgcagccacg
    27721 ccgacgtttc gcaaatccag cgccgaatcg agttgatgca ttacatggtc gttataggta
    27781 aagctcacct tttcaccgtt cgggctaaag acatgcacat ggctgccgcc gcgtagtgct
    27841 cctggggtgt acggcgcggt aatatccatt gcatcgagat tgctcacctt gctgccttca
    27901 acaatcactc cacgccgatg atgaaagtcg taataccatg tttcatcagg attttccggg
    27961 ccgtggatga aaacatattt ctctgacttc ggatgaacgg tcaccacgcc gacgtacgcg
    28021 ccctgtgacg cgcgatagat aacctcgacc tcgccggtat ggatattcac acgctcaatg
    28081 gtctcgccgg taaacgacgc gccagaagga cgcacgtcaa ataccagcca ctggctgtcg
    28141 ggcgtccagg tattggtatt ggtgagcagg tgattacggg gagcaaaggt tatctgtttc
    28201 atggcgcggt cctgatacaa aacgggtccc gccatcatac ttcacaagga cttcactttc
    28261 agacaatagt ctttcgccat cgtgatgaat gtgagtgaaa agatggaaca tttcgacgtg
    28321 gcgattattg gcctcggccc ggcagggtcg gcgttggcac gaaagttagc aggaaaaatg
    28381 caggtgatcg cgctggataa aaagcaccag tgtggtactg aaggtttcag caaaccttgt
    28441 ggcggtctgc tggcaccgga cgcgcagcga tcttttattc gcgatggact gacgcttcct
    28501 gtcgatgtca tcgccaaccc gcagattttc agcgtcaaaa ccgtcgacgt cgccgcatcg
    28561 ctcacgcgta actaccagcg aagctatatc aatattactc gccatgcttt cgacttgtgg
    28621 atgaaatcgc tgatccccgc cagcgttgag gtttaccacg acagcctgtg tcgaaaaatc
    28681 tggcgtgagg atgataaatg gcatgtcatt tttcgcgcag acggctggga gcagcatatt
    28741 accgcccgct atctggtcgg tgccgatggt gccaactcga tggtgcggcg acatctctac
    28801 ccggatcatc agattcgtaa atatgtcgcc atccagcagt ggttcgcaga gaaacatccg
    28861 gtaccgttct actcctgcat ctttgataat gcgataactg actgttactc atggagtatc
    28921 agcaaagacg gttattttat ctttggcggt gcctatccga tgaaagacgg tcaggcgcgt
    28981 ttcgcggcgc tgaaagagaa aatgagtgcc tttcagttcc agtttggtaa ggcggtgaaa
    29041 agcgaaaaat gcacggtgct gtttccctca cgctggcagg attttgtctg cggcaaggac
    29101 aacgcctttc tgattggcga agcggcggga tttatcagcg ccagctcgct ggaggggatt
    29161 agctatgcgc tggatagcgc agagattctg cgttcggtat tactgaagca gccagagaag
    29221 ctcaataccg cttactggcg cgccactcgc aaactgcgtt taaaactctt cggcaagata
    29281 gtaaaaagcc gatgcctgac cgcaccggct ttaagaaagt ggattatgcg cagtggagtg
    29341 gcgcatattc cacagttgaa agattatcca acgcgcttca catcgcccac cagcatgatg
    29401 taagagagcg cgccaatcaa cgcgacggcg gagatataaa ccagtgcagg tgcgaaaccg
    29461 taaccctgcg ccaggtaccc caccaccagc ggaacagtga tgccgcccag accaccggca
    29521 aagttaaaca cgccgccggt taaaccaatc aggcgcatcg gtgccagaga agaaaccagc
    29581 gaccaggtaa tcgaagcaaa accgttgccg aagaatgcca gcgccatcag gcacataatc
    29641 atcatcggat cgttggtgta gttagcgccc ataatgcagg tggagatcag caagccgcag
    29701 ataatcggcg ttttacgcgc aaagcccagt gaaaagccct tacgtaccag cagatccgcg
    29761 acccagccag agagcaggac gccaataaac gccgcgagga acggcaccgt ggtcataaag
    29821 cctgctttca gcgctgtgat tcctttttcc tgggttaaat agttcgggaa ccaggttaag
    29881 aaaaaccaca gtgtagaagc caccgcaaat tgcccaagat agacaccgat cagtttacga
    29941 tggaacacca gtttccagtc tttggctgtt aacggctgac gcgcctcttt cttcaccggc
    30001 gcatcgccat ccaccagacc gccgccatca cgaatgtaat ccagttcagc tttgctgatg
    30061 cctttggtca gacgcggcgg ctgataaacc ttaaaccaaa tcagcgacca gataatgccg
    30121 ataccaccgg tgacaatgaa cacccagtgc cagctcaaca tctcctgaat ccagatcagc
    30181 agcggagtca gaaacgccag accgacaaac tgaccagacg tataaaaacc aacggcagaa
    30241 gcacgttcat gttccgggaa ccaactggtt accatccggt tattggtcgg gaacgcgggc
    30301 gcttcgaaaa taccggttat cgcgcgcaga ccaattaatg acattaagcc cgtggcaaag
    30361 ccctggaata acgtcgccac tgaccagcca aatatcgcaa taaaataagt cacgcgtgaa
    30421 cctacgcgat ctaaaaacca accaccgggg atctgacata gcgtataaag ccaggcgaag
    30481 gccgaaaata catagcccat ttgcgcttta gtaataccaa actcttcctg aatatgggca
    30541 gaagccacgg ccaggttagc gcggtcaaca taacaaatga ccaccgtaat aaagatcatc
    30601 accagcgtca gataacgccg acgccccggc tttgctgcat taacgggaat atccatagcg
    30661 agctttctcc agattttggg catagcgaag ccgctcacca tgccctgtaa tttacagagg
    30721 gttatatttt tgtattgctg ttttagtgcc cgatgagggg cttacgtggc aggaattacc
    30781 actctgctac gctgttatct tcatgacgcc agagcggatt acgccagtcc ggggcatttt
    30841 tactgaactc aataacttta gcttcgtcga tttccacgcc taagcccggt ttcgttaatg
    30901 gtttaaagaa accgccgacc atgctgaagt cctctttgtt tttcacaaag tcgagtaact
    30961 ccgcgccttt gttgtaatga atgcccatac tttgttcctg aaggacggcg ttataggaaa
    31021 caaagtcgat atgcaggcaa gccgccagtg caatcggtcc gagcggacag tgcggcgcaa
    31081 gggtcacgtc ataggcttct gccattccgg caattttgta gcattcggta ataccgcctg
    31141 cgtgggagag atccggttgc agaatcgaaa taccgcctgc ctccagcaca cgtttaaaat
    31201 cgaagcgtga gaacatgcgc tcacccgccg ccagtggaat atgcgtttgt gccgccagtt
    31261 tcgggtagta ttcggcctgt tccgccagca ccggctcttc aataaacagc gggcgatacg
    31321 gctccagttc tttaatcagc actttcgcca tcggcgcgct gacgcgacca tggaagtcca
    31381 gaccaaactc aatctgatta ccaaaggctt cacgaatttg cgccacggtg ttgactgccg
    31441 catctaccgc gcgggagtta tcaattagcc ccagttcttc acaaccgttc agtttgaagg
    31501 tatcgaagcc gatttcgcgt agcgttttaa tgccgtcgat aacatccgcc ggacgatcgc
    31561 cgccgaccca actgtaggct ttaattttgt cgcgaaccag gccgcccatc agttgccaga
    31621 ccggcgcatt cagcactttg cctttgatat cccataacgc ctggtcgatc ccggcgatgg
    31681 cactcatcag aatcggaccg ccacgataaa atccggcgcg atacatcact tgccataagt
    31741 cgttgatgcg tgacgggtcc tgaccaatca aatagtcact cagctcgtgg actgccgctt
    31801 ccaccgtgcg agcgcggcct tcaatcacgg gctcgcccca accgaccaca ccttcatcgg
    31861 tttcaatttt caggaacatc cagcggggag gtaaccgata cgtggtaatt ttggtgattt
    31921 tcattgcact gcctctcgat acgccttaac aaatgctgct gcctgctgcg cagtccgttc
    31981 tacggactgc ccggcgcgat agagatcgct acctaagccc gcccctgcac aacctgcgtc
    32041 tatccactgc gccaggtttt ctggcgtcac gccgccaacg gcaaagactg cgatgtccgg
    32101 tggtaatacc gcttttaatg ctttgatgta ttgcggacca aaagccgatg acggaaatat
    32161 tttcagcgcc tgcgcgcccg cttcgagcgc ggtaaaggct tcggtcgccg tcgcacagcc
    32221 ggggcagacg gtcatgccgt agcccaccgc acggcggatc acttcactat ggatattggg
    32281 cgtaacgatg agctgacagc ccattctggc gagcgcatcg acctgttcag gtttgagtac
    32341 cgtacctgcg ccaatcaacg ccttgtcgcc atacgcatca acgatggcgg gaatgctctg
    32401 ctcccattgt ggagaattca gcgggatttc aaccgcatcg aacccggcgt caatcaccgc
    32461 gccaacatgt gccagcgcct cgtcgggcgt aatgccgcgc aaaatggcga tcagcgggag
    32521 tttagtttgc cactgcatga gcgatgctcc ttataccagc ctgaaatgcc gtgtcgcctg
    32581 ccaccgccgt cacgtcgcaa cccatcgcct gaaaggcttg ctggtagcgc gcggtcagcg
    32641 atgttccggc gacaagggtg atgacttgtt gatgggtcac atagtcgcgc atactggcga
    32701 cctctgcgcc aatcaacaaa ccagagagaa attcgctgac ctgttcgcgg ggaagtgttc
    32761 ccagcacatg cgaggcgcga acttcaaaaa gctgcggcaa tatgtcggga gcattaaggc
    32821 cacgctcaag accagccgcg aaagcatcgg cagcgttttc ctgcggcggc aaacctgcgc
    32881 caatcaatga gtgatttaac agtaaatgat gtaattcacc ggtcatcacg gtgcgaaaat
    32941 cgttaatttg ctggctatcg gcttgcaccc atttgcaatg cgtaccgggc atgacataaa
    33001 gagaggaagg agcgagagtg cgcgcgccga tcaattgtgt ttcttcgccg cgcatcacat
    33061 tgtggttatc gtcatgagag acgcataatc cgggaataat ccagatattg tcgccaacag
    33121 acgttaattg ttcgccaata gacgaaaaac gggcaggaac ggataaatac ggcgcaactt
    33181 tccagccgac attgctgccg accattcctg ccattaccac tggcgtattc tcttcacgcc
    33241 agtcggtcgt cacttctgct aacaccgcag ccggagattt tccgttcagg cgcgtgacgc
    33301 ctgcttctga ttgcctgctg tccaggcagt ggtcgccctg ataaagccag gcgcgcagat
    33361 tggtcgatcc ccagtcaatt gcgatgtagc gagctgtcat gtgatttcct ttaaccttcg
    33421 tgtcgagctg gcgatcatgg taagcgccgc ctgctctgcc gcatcgccgt cctgatgcct
    33481 tatcgcatcg aacagcgcct tatgttcctg gagcgtttgc ggcatgttgg cctcatcgcc
    33541 catccaggtt cgttcaaaaa ccgcccgctg cagcgaactg atcgcaatgc tcagttgctg
    33601 taacaccggg ttatgcaccg actgcagcac cgcctcgtgg tagcgaatat ccgcttcgtt
    33661 aaacgcttcg cggtcctgat tgttagcaat catctcgttc agcgccgatt caatctgcgc
    33721 cagatcgctg gaggtcgcgc gctctgctgc ccaacgggca atcgccggtt ccaccagatt
    33781 tcgcacttcg ctcattgcac tgataagccg tgggtcgtag tcgttttcca gcacccattg
    33841 cagtacgtca gtgtcgaggt aattccactg gttacgcgga gccacaaacg ccccgcgata
    33901 acgtttcatt tcaatcagcc gcttcgccat cagcgaacga aatacctcac ggatgatgtt
    33961 gcgcgaggtt gcaaactcct cacagagttc tgcctcagcc ggaagcggcg agcctggcac
    34021 gtatttgccg tgaacgatct gtctacccag cgtaataacg atgcgatcgg ttttattaag
    34081 agtcatggag agtccttgtg ctctgtatgt tcttctctac tttaccccga tcgatgcata
    34141 acgcggcaat tttgtagtac cagcgtgatg acgttcgcgt ttgctgtgcg cctaatgtag
    34201 tacaaagtta tattgttgta ctacaattta gatcacaaaa agaacaatcc ataagaaaat
    34261 gttctacgtc gggcaaaaat ctgaaaaggg agatcaggcg ctaaactggg gggaaagaag
    34321 agtatgcttt caacggctta gtactcttgt ttaaaggatt aatcatgaag ttgaatctta
    34381 agggattttt caaagctgcc agtttgtgtt ctttggcatt tgcgttgacc ggatgtatca
    34441 cctggggatt ggtttccaat actgcaagcc gtccacatgc cgagcaatgg cggtcagaca
    34501 cgattaaggg tttatcgctg gcagaagata gtaacggtac gaaaggctat gtttttgttg
    34561 gtgaatcgtt ggactatctg ttgaccaccg gcggcgatga ggtagttaac atgctgaacg
    34621 atccggcaat ccatggtgaa agaatcaccg tttctgataa cgcgaaattt attttaagca
    34681 gcagtaataa gaatttctct ggtgctatta cgctctatta cgactggaac aacgaagaag
    34741 ataaggcact ggcaacacaa tatggtttta tttgtgatac ccggcgttgt acatggatgc
    34801 tggacggttt gaaaggctca atccaccaga aaaataaaaa agcggattac tcaaacgtga
    34861 tggttttcca tcaacccttt acggtgggat tttatgagta caaagcaaca gatggcgtcc
    34921 cgcacggcct cgttaatgcg ctgctgccag tcactcttac gctggatata gtaacgtcac
    34981 cactccagtt cctgatttta tgcacgacca gaaattgtta atcgctgcgg gcaaacgcat
    35041 tcatgccgtt tgcccgttca ctgattaatt cagcacatac ttctcaatag caaatgccac
    35101 gccatcttca aggttagatt tggtgacaaa gttcgccact tctttcaccg aaggaatagc
    35161 gttatccatc gccacaccga cgcctgcata ttcaatcatt gcgatgtcgt tttcctgatc
    35221 gccaatcgcc atgatttctt ccggtttaat acctaacacg tcggccagtg atttcacacc
    35281 cgtacctttg ttaacgcgtt tatcgaggat ttcgaggaag tacggcgcac ttttcagcac
    35341 ggtgtatttt tctttcactt cctgcggaat acgcgcaatg gcctggtcga ggatggcggg
    35401 ttcatcaatc atcatcactt tcaggaactg ggtattggag tccattttct ccgcttcgca
    35461 gaacaccagc ggaatggtgg caacgaaaga ttcatgcacc gtgtagtagc tgatatctcg
    35521 gttggcggtg tacagcgtgg tgcggtccag ggcgtggaaa tgagaaccga cttcgcggga
    35581 gagtttttcc aggaaacggt agtcgtcata gctgagagcg gtttgcgcca cggtgctacc
    35641 atcagcggcc ttctgtacca gcgcgccgtt ataagtaatg cagtagtcgc ccggctgttc
    35701 catatgcagc tctttcaggt aattgtgaac acctgcataa gggcgacccg tcgttagcac
    35761 gacattcacg ccacgggcgc gagctgcggc aatcgcattt ttaacggcgg gtgaaatggt
    35821 gtgatcgggc agcagaaggg tgccatccat atcgatagca atgagtttga tagccatgag
    35881 ttccccaggt agattggttc ctgacccatg ctaacgcgat tccgctcaaa aatcagtaca
    35941 acacccgagg gaaaaggggg atgcaacgcg cattgcgtgc tccccatttg cttagcggaa
    36001 aagtttccct ttcagcagtt ccatgcctgc ggaaagcaga tcgttattgg cttgtggtga
    36061 cacttcacct tgcggtgaga gcgcatcaat aatcttcggc aattgttctg ccagtaaact
    36121 ggaagctgta ctggtatcca cgccaagttt ttgcccgaga tcggacaccg catttgtgcc
    36181 gagcgccgat tccacttgct cgccgctaac tgattgattg ctctgttgat tactcagcca
    36241 ggttgagaga atggccccta agccgccact ttgcagtttt tccagcagca cttgaatgcc
    36301 gccctgttcc tcaacccagc tcaaaatggc ctgatatttc cccgcatcgc ctttcagaaa
    36361 ggcaccgaca acgtcatcaa aaagccccat gataatcacc tgtaaagcgt tacgtgttga
    36421 cccaaaaagt atagatctgc gggatgataa ttgcggattg cagaaataaa aagggcggag
    36481 atgatctccg cccttttctt atagcttctt gccggatgcg gcgtgaacgc cttatccggc
    36541 ctacaaaaac atgcaaattc aatatattgc aggagtctcg taggcctgat aagcgtagcg
    36601 catcaggcac gctcgcattg ttaacgccat taaatatcga tattcgccgc tttcagagcg
    36661 ttctcttcga taaacgcacg gcgcggttca acggcgtcgc ccataagcgt agtgaacaac
    36721 tggtcggcag caatcgcatc tttaacggta acgcgcagca tacgacggct ttccgggtcc
    36781 atggtggttt cccacagctg ttccgggttc atctcgccca gacctttata acgctggatg
    36841 gagaggccgc gacgggactc tttcaccagc cagtccagcg cctgctcgaa gctggctacc
    36901 ggctgacgac gttcgccacg ttcgataaat gcatcttctt ccagcaagcc acgcagtttc
    36961 tcacccagcg tgcagatacg acgatattcg ccgccggtga taaattcgtg atccagcgga
    37021 tagtcagtat ccacaccgtg ggtacgcacg cgaacaatcg gctcgaacaa gttttgctca
    37081 gcattggtgt ggacatcaaa cttccactgg ctgccgtgct gttctttgtc gttcagttcg
    37141 ctgaccagcg cgttcaccca gcgggtaacg gtctgctcat cagagagatc ggcttccgtc
    37201 agcgtcggct gatagataag ctctttcagc attgccttcg gataacgacg ctccatgcgg
    37261 ttgatcattt tctgcatcgc gttgtactca gacaccagtt tctctaacgc ttcgccagcc
    37321 agcgccggtg cactggcgtt ggtgtgcagc gttgcgccat ccagcgcgat agagatctgg
    37381 tactgatcca tcgcttcgtc gtctttaatg tactgttcct gcttaccttt cttcactttg
    37441 tacagcggcg gctgagcgat gtagacgtga ccacgttcaa cgatttccgg catctgacga
    37501 tagaagaagg tcaacagcag cgtacgaatg tgcgagccgt cgacgtccgc atcggtcatg
    37561 atgatgatgc tgtgataacg cagtttgtcc gggttgtact cgtcacgacc gataccacaa
    37621 ccaagcgcgg tgataagcgt cgccacttcc tgagaagaga gcatcttatc gaagcgcgct
    37681 ttctcgacgt tgaggatttt acccttcagc ggcagaatcg cctggttctt gcggttacgc
    37741 ccctgcttcg cagagccgcc cgcggagtcc ccttccacaa ggtacagttc ggaaagcgcc
    37801 ggatcgcgtt cctggcagtc tgccagtttg cccggcaggc cagctaaatc cagcgcacct
    37861 ttacggcggg tcatttcacg cgcgcgacgc gcagcttcac gggcacgggc agcatcgata
    37921 attttgccaa ccacgatttt cgcgtcggtt gggttttcca gcaggtattc cgccagcagt
    37981 tcgttcatct gctgttcaac cgccgatttc acctcagaag aaaccagttt gtctttggtc
    38041 tgtgaggaga atttcgggtc cggcactttc acggaaacga ccgcaatcag gccttcacgc
    38101 gcatcgtcac cggtggcgct gactttggct tttttgctgt agccttcttt gtccatgtag
    38161 gcgttcaggg tgcgggtcat cgccgcacgg aagcctgcca ggtgagtacc gccgtcacgc
    38221 tgcggaatgt tgttggtaaa gcagtagatg ttttcctgga agccatcgtt ccactgcaac
    38281 gccacttcga cgccaatacc gtctttttcg gtggagaagt agaagatatt cgggtggatc
    38341 ggcgttttgt tcttgttcag atattcaacg aacgccttga tgccgccttc atagtggaag
    38401 tggtcttctt tgccgtcgcg cttgtcgcgc agacgaatgg aaacgccgga gttgaggaac
    38461 gacaactcac gcagacgttt cgccagaatg tcatattcga actcggtcac attggtgaag
    38521 gtttcaaggc ttggccagaa acgcaccata gtgccggttt tttcagtctc gccggtaacc
    38581 gccagcgggg cctgcggtac accgtgttcg tagatctgac ggtgaatttt accctcgcgc
    38641 tggataacca gctccagttt ttgcgacagg gcgtttacta ccgaaacacc aacgccgtgc
    38701 agaccgccgg acactttata ggagttatcg tcaaatttac cgcctgcgtg cagaacggtc
    38761 atgatcactt ccgccgccga tacgccctct tccgggtgaa taccggtcgg aatgccgcgc
    38821 ccgtcatcct gtacagagac agagttatcg gcgtgaatgg tgacgataat ttctttacag
    38881 tgacccgcga gcgcttcgtc gatagcgtta tctaccacct cgaataccat gtggtgcaga
    38941 ccggtgccgt catccgtgtc gccgatatac atacccgggc gcttacgcac cgcatccagc
    39001 cctttcagga ctttgatact ggaggagtca taagaattcg acatcaacgt ttctcgctca
    39061 tttatacttg ggttaatccg ttattttacc cttttccacg gtaaacatct tcgaattttc
    39121 gtccgacatg tctataacgt gttcagcact gatcgcgctg acaaagacct gtgattgcgt
    39181 cgcttttaag cggctggcaa gcagcccgcg acgctcatca tcaagctcag aggcaaaatc
    39241 atctatcagg tagagacacc gccgcccgct ttcacgggtg aggaactctc cttgcgccag
    39301 acgtaaggcg cacatcaaca gcttaagctg cccacgcgat aaggtatctt ccaccggcgc
    39361 accgtcggcg cgaatgcgta agtccgcttt atgcgggccg tgcgcggtgt aggttagctg
    39421 gcgatcgcgt tcaaaattac gttccagcac ctcagcgtac tctgtctctt tctcccagcc
    39481 gcgctggaaa gagaaagtca gagaaaactc agggagaaat tgcttacagg tatcagccat
    39541 atcggctgcg ataccggcgc tatactccgc gcgccaggtg ctgatttgct ccgccagcgg
    39601 gatcagttct ttatcccacg gacgtagctg ttcgtaacga gtcacctggc gcagcgccgc
    39661 attgcgctgc ttgagcaatc gcttgagatt gctccaggcg gtgaaaaatc cgggttcgtt
    39721 gtgaaagcat ccccagtcga ggaatgctct tctgtatttg gggccgccgt tgagtaaagt
    39781 aaacccttct ggcgttatca gctgcattgg catcaggtgc gccagctccg cgactttatg
    39841 cccgtctgta ccgtcaatac ggactttgct atcgccttgt ttgtctttgg ttaaaccaat
    39901 cgccgtctcg cgctcttcgc cctgtaatcg cccgtggaga acaaacgcct cctgctcgtg
    39961 gcgaatgacg cgaccaatct gcaaactgcg aaacgcccga ccgtggccga gcgtatagat
    40021 ggcttccagc acgctggttt tgccactgcc gttggcacct accagaaagt taaagccggg
    40081 agataaggcg agatccgcgg tttcaatgtt gcggaaatcg cggatcaaca agcgggtgag
    40141 ggacattaca gtctcattgg catgacaaca taagccgcgc tctggctggc cgcatcttca
    40201 atctgcacgc tggaaaccga atcggtcagc atcatgcgga cgttttcgca tttcagcgcg
    40261 ttgagaacat ccagcacata gctgacgttg aagccgattt ccatctccgc accgctatag
    40321 gtaacgtcga ggatctcttc cgcttcttcc tgttctgggt tgttggcggt gattttcagc
    40381 tggttttcgc tgacatacag acgcacgccg cggaatttct cgttagagag aattgccgca
    40441 cgagcaaacg cctgcttgag cagatcacag ccagcttcca gatgtttgtc cgggttcttc
    40501 ggcagaacgc gacggtaatc cgggaagcga ccatccacca gtttcgaggt gaagataaag
    40561 tcgccaacgt gggcgcgaat gttgttgctg ccaatctgca cgcgcagcgg attgtcgccg
    40621 ccgtcgagca tacgcatcag ttcaatcacg cctttacgcg gcacgatcac cgaatggctt
    40681 ggcaaagatt gaccaattgg cattgaacag actgccagac ggtggccgtc ggtcgccacg
    40741 gtgcgcagtt cttcaccttc ggtttcaaac agcataccgt ttaagtaata gcgaacgtcc
    40801 tgatgcgcca tagaaaactg ggtcgcttca atcagacgct tcatcgtcgc ctgcggcagg
    40861 gtaaattcga cttcgctctg ccagtcatcg aggttcggga aatctgccgc tggcagggta
    40921 gacagcgaaa aacggctacg cccggagcgc accagcatcc gctcaccttc cagctgcacg
    40981 gcaatttccg cgccttcagg cagaccacgg cagatatcaa agaatttgcg cgccggaacg
    41041 gtcgtcgccc ctggttcgtg cggctgaacc agcgcaacgc gtgccaccat ttccatctcg
    41101 agatcagtac cggtcagcga caacgtaccg tcagcaacct gtaacagcag attaccgaga
    41161 atcggtagcg taggacgacc acctaacgga ccgctcacct gttgtagcgg ttttaataaa
    41221 tgctcacgtt ctacggtaaa tttcataggt ttacgatgac aatgttctga ttaaattaga
    41281 aaaatcttct ttgatatcgt ggctctcttc acgcaactgc tcgatcttcc ggcaggcatg
    41341 aagcaccgtc gtgtggtcac gaccaccaaa ggcatcgcca atctccggca gactgtggtt
    41401 agtcagctct ttcgccagcg ccatcgccat ctggcgcgga cgggccaccg agcgggatcg
    41461 acgcttggaa aggagatccg cgactttgat cttgtagtac tccgccaccg tcttctgaat
    41521 attgtcgatg gtgaccagtt tttcctgcaa tgccagcaag tcgcgcagcg cctcacgcac
    41581 gaagtcgatg gtgatcgccc gtccggtaaa gttggcattg gcaatgacgc ggttcagcgc
    41641 gccttccaac tcacgtacgt tagatcgtag acgcttggcg ataaaaaacg ccacttcgcc
    41701 cggcaaacga atgtcgtttt cgtcggcctt tttcatcagg atcgccacgc gagtttccag
    41761 ctctggcggt tcgatcgcca cagtcagtcc ccagccgaag cgggatttca aacgatcctc
    41821 aacgccgttg atctctttcg gatagcgatc ggaggtgaga atgatctgtt gattaccttc
    41881 cagcagggcg ttgaaggtgt ggaaaaactc ttcctgagat cgttctttat tagcaaaaaa
    41941 ctgaatatcg tcgatcagca gtgcatctac ggaacggtag tagcgtttaa actcttcgat
    42001 cgcgttgttt tgcagggctt taaccatgtc ctgaacaaag cgctcggagt gcatataaac
    42061 cactttggca ttcggcttgc gcgccataat gccgttaccc accgcatgca gcaggtgagt
    42121 tttacccaaa cccgtgccgc cataaaggaa caacgggtta taagcaccgc cagggttatc
    42181 tgccacctgg cgagccgccg cgcgcgccag ttggttagat ttaccttcaa cgaagttatc
    42241 aaacgtgtgt ttgacgttta cgttagaacg ataggtcggt tctgccggag ccgggacgtt
    42301 atcccaaccc gagcgcgtag aaggcgcagc acgttgcggc tgcgtttgcg ccacctgtgc
    42361 aggggccgcg acgttgctcg tcactgccgc ttgtggcgtt tgcgtcaccg gtttggtgcc
    42421 gacttcaaaa cgcagctgtg gggcatccgc tccgcagaaa ctggttagca gtccattgat
    42481 attattaagg tacttgtccc gtacccaatc gaggacaaaa cggtttggcg cgtacagggc
    42541 cagcgtgtta tcgctcagtt ccgcctgcaa tgggcgtatc cacatactga attctgtggc
    42601 tggtaactca tcctgcaatc gggcaagaca ctgctgccaa agcgaaagtg acacggcgga
    42661 ctccactcga acaaaagtcg ataatgacta aggctgaaac attcatgatt gttgacgtac
    42721 gtcgaaaaga ccctgcatga gggtgacgca cgaaccgctg tctgcggtta tatgcccgat
    42781 caagatcctg caaaacgatc gggaccgcgg atcatagcct aaactgcgca agagatcttc
    42841 tgtttctcac agattttccc gatttatcca caggatgatc cagaactcgc taagtgtaaa
    42901 cgatcctgcc gcgggccggg cacgatttac gccgcatatt ggaaaattta atgaccatag
    42961 acaaaaattg gcttaatcga tctaataaag atccaggacg atccttgcgc tttacccgtc
    43021 agcccgtata atcctccacc cggcgcgcca tgctggtttt cactggtgtg aggccgtacg
    43081 ttttccccgc gaaaaggtgc ggaaaagcgc ggtaaataag gaaagagaat tgactccgga
    43141 gtgtacaatt attacaatcc ggcctcttta atcacccatg gcttcggtgt ccatcgtttc
    43201 atttttcggc ggatatccaa taaagccatt gaatttattc aagtttaggt agaaatcgcc
    43261 atgaaacgca cttttcaacc gtctgtactg aagcgcaacc gttctcacgg cttccgtgct
    43321 cgtatggcta ctaaaaatgg tcgtcaggtt ctggcacgtc gtcgtgctaa aggccgcgct
    43381 cgtctgaccg tttctaagta ataaagctaa cccctgagtg gttaagctcg catttcccag
    43441 ggagttacgc ttgttaactc ccagtcaatt cacattcgtc ttccagcagc cacaacgggc
    43501 tggcacgccg caaattacca ttctcggccg cctgaattcg ctggggcatc cccgtatcgg
    43561 tcttacagtc gccaagaaaa acgttcgacg cgcccatgaa cgcaatcgga ttaaacgtct
    43621 gacgcgtgaa agcttccgtc tgcgccaaca tgaactcccg gctatggatt tcgtggtggt
    43681 ggcgaaaaaa ggggttgccg acctcgataa ccgtgctctc tcggaagcgt tggaaaaatt
    43741 atggcgccgc cactgtcgcc tggctcgcgg gtcctgatag ccctcattcg ggtctatcaa
    43801 cgcctgatta gtccgctact cgggccgcat tgtcgtttca ctccaacctg ttcaagctac
    43861 ggaattgagg cattgcgcag gtttggagtg ataaaaggca gttggttgac ggtgaaacgc
    43921 gtattaaaat gccacccttt acaccctggt ggtgacgatc ccgtcccgcc cggaccattt
    43981 gataccagag aacactaacg atggattcgc aacgcaatct tttagtcatc gctttgctgt
    44041 tcgtgtcttt catgatctgg caagcctggg agcaggataa aaacccgcaa cctcaggccc
    44101 aacagaccac gcagacaacg accaccgcag cgggtagcgc cgccgaccag ggcgtaccgg
    44161 ccagtggcca ggggaaactg atctcggtta agaccgacgt gcttgatctg accatcaaca
    44221 cccgtggtgg tgatgttgag caagccctgc tgcctgctta cccgaaagag ctgaactcta
    44281 cccagccgtt ccagctgctg gaaacttcac cgcagtttat ttatcaggca cagagcggtc
    44341 tgaccggtcg tgatggcccg gataacccgg ctaacggccc gcgtccgctg tataacgttg
    44401 aaaaagacgc ttatgtgctg gctgaaggtc aaaacgaact gcaggtgccg atgacgtata
    44461 ccgacgcggc aggcaacacg tttaccaaaa cgtttgtcct gaaacgtggt gattacgctg
    44521 tcaacgtcaa ctacaacgtg cagaacgctg gcgagaaacc gctggaaatc tccacctttg
    44581 gtcagttgaa gcaatccatc actctgccac cgcatctcga taccggaagc agcaacttcg
    44641 cactgcacac cttccgtggt gcggcgtact ccacgcctga cgagaagtac gagaaataca
    44701 agttcgatac cattgccgat aacgaaaacc tgaacatctc ttcgaaaggt ggttgggtgg
    44761 caatgctgca acagtatttc gcgacggcgt ggatcccgca taacgacggt accaacaact
    44821 tctataccgc taatctgggt aacggcatcg ccgctatcgg ctataaatct cagccggtac
    44881 tggttcagcc tggtcagact ggcgcgatga acagcaccct gtgggttggc ccggaaattc
    44941 aggacaaaat ggcagctgtt gctccgcacc tggatctgac cgttgattac ggttggttgt
    45001 ggttcatctc tcagccactg ttcaaactgc tgaaatggat ccatagcttt gtgggtaact
    45061 ggggcttctc cattatcatc atcaccttta tcgttcgtgg catcatgtac ccgctgacca
    45121 aagcgcagta cacctccatg gcgaagatgc gtatgctgca gccgaagatt caggcaatgc
    45181 gtgagcgtct gggcgatgac aaacagcgca tcagccagga aatgatggcg ctgtacaaag
    45241 ctgagaaggt taacccgctg ggcggctgct tcccgctgct gatccagatg ccaatcttcc
    45301 tggcgttgta ctacatgctg atgggttccg ttgaactacg tcaggcaccg tttgcactgt
    45361 ggatccacga cctgtctgca caggacccgt actacatcct gccgatcctg atgggcgtaa
    45421 cgatgttctt cattcagaag atgtcgccga ccactgtgac cgacccgatg cagcagaaga
    45481 tcatgacctt tatgccggtc atcttcactg tgttcttcct gtggttcccg tcaggtctgg
    45541 tgctgtacta tatcgtcagc aacctggtaa ccattattca gcagcagctg atttaccgtg
    45601 gtctggaaaa acgtggcctg catagccgcg agaagaaaaa atcctgattc ggtgagtttt
    45661 cgctaaaata agggcggtca gttgaccgcc ttttttcttt tcgtagggcg gataagcacc
    45721 gcacatccgc cacacaaagc aacaggaaca tcatgagcga taatgacact atcgtagccc
    45781 aggccacgcc tccgggacgt ggcggcgttg gcatcctgcg catctccggc ctcaaagccc
    45841 gtgaagttgc cgaaaccgtg ctgggtaaac tgcctaagcc acgctacgcc gattatcttc
    45901 cgtttaaaga cgccgatggc agcgtgctcg atcaggggat tgcgctatgg ttccctggcc
    45961 cgaactcgtt caccggcgaa gatgtgctgg aactgcaagg tcatggcggt ccggtgatcc
    46021 tcgacctgct gttaaaacgc attctgacca ttcccggcct gcggattgcc cgccctggtg
    46081 agttttccga acgcgcattt ctgaacgata aactcgactt agcccaggcc gaggcgattg
    46141 cggatcttat cgacgccagc tcggaacagg cggcccgttc ggcgctaaac tcgctgcaag
    46201 gcgcgttctc cgcacgggtc aaccatctgg tggaagccct cacccacttg cgcatttacg
    46261 tcgaagcggc aattgatttc ccggatgaag agatcgattt cctctccgac ggcaaaattg
    46321 aagcccagct caatgacgta attgccgatc tcgacgcggt acgcgccgaa gctcgtcagg
    46381 gtagtttgtt gcgcgaaggg atgaaagtgg tgattgccgg acgtcctaac gccggtaaat
    46441 cgagcctgct gaacgcgctg gcgggccgtg aagcagcaat cgtaaccgat atcgccggaa
    46501 ccacgcgtga cgtgctgcgt gagcatatcc acattgacgg aatgccgttg catatcatcg
    46561 ataccgccgg gctacgtgaa gccagtgacg aagttgaacg tattggtatc gagcgcgcat
    46621 ggcaggaaat tgaacaggca gaccgcgtgc tgtttatggt cgatggcacc acgacagacg
    46681 ccgttgatcc ggcagagatc tggccggaat tcatcgcccg tctgccagcg aaactgccga
    46741 tcaccgtggt gcgcaataaa gccgatatca ccggcgaaac gttggggatg agcgaagtga
    46801 acggtcatgc gttaattcgt ctttcggcgc ggactggcga aggcgtggat gtactgcgta
    46861 accatctcaa acagagcatg ggctttgaca ccaatatgga aggcggcttc ctggcgcgtc
    46921 gtcgtcacct acaggcgctg gaacaggcgg cagagcattt gcagcaaggt aaagcgcaac
    46981 tgttaggtgc ctgggcgggt gaactgctgg cggaagagtt gcgcctggcg cagcagaact
    47041 taagcgagat caccggggaa tttacttcag acgacctact ggggcggatt ttctccagct
    47101 tctgtatcgg taagtaaccg cgcttgcgaa gccgcattct gactgtcaga tgcggcttcg
    47161 cttcattgtt agcactcctg ttattcct
//
LOCUS       DAZFJL010000018        46573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFJL010000018 DAZFJL010000000
VERSION     DAZFJL010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.018"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            186..1403
                     /gene="alaA"
                     /locus_tag="ACHJ8C_001030"
     CDS             186..1403
                     /gene="alaA"
                     /locus_tag="ACHJ8C_001030"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO5550926.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1487..2086
                     /gene="yfbR"
                     /locus_tag="ACHJ8C_001031"
     CDS             1487..2086
                     /gene="yfbR"
                     /locus_tag="ACHJ8C_001031"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO5550927.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELQDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2145..3977)
                     /gene="yfbS"
                     /locus_tag="ACHJ8C_001032"
     CDS             complement(2145..3977)
                     /gene="yfbS"
                     /locus_tag="ACHJ8C_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO5550928.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4064..4714)
                     /gene="hxpA"
                     /locus_tag="ACHJ8C_001033"
     CDS             complement(4064..4714)
                     /gene="hxpA"
                     /locus_tag="ACHJ8C_001033"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO5550929.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLSPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4725..5219)
                     /gene="yfbU"
                     /locus_tag="ACHJ8C_001034"
     CDS             complement(4725..5219)
                     /gene="yfbU"
                     /locus_tag="ACHJ8C_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO5550930.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5301..5756)
                     /gene="yfbV"
                     /locus_tag="ACHJ8C_001035"
     CDS             complement(5301..5756)
                     /gene="yfbV"
                     /locus_tag="ACHJ8C_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO5550931.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6094..7296
                     /gene="ackA"
                     /locus_tag="ACHJ8C_001036"
     CDS             6094..7296
                     /gene="ackA"
                     /locus_tag="ACHJ8C_001036"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO5550932.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHKRNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7371..9515
                     /gene="pta"
                     /locus_tag="ACHJ8C_001037"
     CDS             7371..9515
                     /gene="pta"
                     /locus_tag="ACHJ8C_001037"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO5550933.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIIANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSTKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRENYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9705..11225
                     /gene="yfcC"
                     /locus_tag="ACHJ8C_001038"
     CDS             9705..11225
                     /gene="yfcC"
                     /locus_tag="ACHJ8C_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO5550934.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPQYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAIFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11259..11801)
                     /gene="yfcD"
                     /locus_tag="ACHJ8C_001039"
     CDS             complement(11259..11801)
                     /gene="yfcD"
                     /locus_tag="ACHJ8C_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO5550935.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11859..12410)
                     /gene="yfcE"
                     /locus_tag="ACHJ8C_001040"
     CDS             complement(11859..12410)
                     /gene="yfcE"
                     /locus_tag="ACHJ8C_001040"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO5550936.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLNFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12466..13110)
                     /gene="yfcF"
                     /locus_tag="ACHJ8C_001041"
     CDS             complement(12466..13110)
                     /gene="yfcF"
                     /locus_tag="ACHJ8C_001041"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO5550937.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13246..13893
                     /gene="yfcG"
                     /locus_tag="ACHJ8C_001042"
     CDS             13246..13893
                     /gene="yfcG"
                     /locus_tag="ACHJ8C_001042"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO5550938.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAVTLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQHGDERSDS"
     gene            13950..14312
                     /gene="folX"
                     /locus_tag="ACHJ8C_001043"
     CDS             13950..14312
                     /gene="folX"
                     /locus_tag="ACHJ8C_001043"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO5550939.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14333..15226
                     /gene="yfcH"
                     /locus_tag="ACHJ8C_001044"
     CDS             14333..15226
                     /gene="yfcH"
                     /locus_tag="ACHJ8C_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO5550940.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLDLGHQITVVTRNPQKASSVLGPR
                     VTLWQGLAEQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRFGLGGPIGSGRQYLAWIHIDDMVNGILWLLDKELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKKL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15274..16176)
                     /gene="rpnB"
                     /locus_tag="ACHJ8C_001045"
                     /pseudo
     CDS             complement(15274..16176)
                     /gene="rpnB"
                     /locus_tag="ACHJ8C_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
     gene            complement(16372..17145)
                     /gene="hisP"
                     /locus_tag="ACHJ8C_001046"
     CDS             complement(16372..17145)
                     /gene="hisP"
                     /locus_tag="ACHJ8C_001046"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO5550941.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSSRLQQFLKGSLK"
     gene            complement(17153..17869)
                     /gene="hisM"
                     /locus_tag="ACHJ8C_001047"
     CDS             complement(17153..17869)
                     /gene="hisM"
                     /locus_tag="ACHJ8C_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5550942.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17866..18552)
                     /gene="hisQ"
                     /locus_tag="ACHJ8C_001048"
     CDS             complement(17866..18552)
                     /gene="hisQ"
                     /locus_tag="ACHJ8C_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO5550943.1"
                     /translation="MLYGFSSVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18642..19424)
                     /gene="hisJ"
                     /locus_tag="ACHJ8C_001049"
     CDS             complement(18642..19424)
                     /gene="hisJ"
                     /locus_tag="ACHJ8C_001049"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO5550944.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19645..20427)
                     /gene="argT"
                     /locus_tag="ACHJ8C_001050"
     CDS             complement(19645..20427)
                     /gene="argT"
                     /locus_tag="ACHJ8C_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO5550945.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGPSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALNELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20693..21262)
                     /gene="ubiX"
                     /locus_tag="ACHJ8C_001051"
     CDS             complement(20693..21262)
                     /gene="ubiX"
                     /locus_tag="ACHJ8C_001051"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO5550946.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAVTLPEDLFARWQGA"
     gene            complement(21357..22874)
                     /gene="purF"
                     /locus_tag="ACHJ8C_001052"
     CDS             complement(21357..22874)
                     /gene="purF"
                     /locus_tag="ACHJ8C_001052"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO5550947.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22911..23399)
                     /gene="cvpA"
                     /locus_tag="ACHJ8C_001053"
     CDS             complement(22911..23399)
                     /gene="cvpA"
                     /locus_tag="ACHJ8C_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO5550948.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23658..24320)
                     /gene="dedD"
                     /locus_tag="ACHJ8C_001054"
     CDS             complement(23658..24320)
                     /gene="dedD"
                     /locus_tag="ACHJ8C_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO5550949.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPLKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKEKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24310..25578)
                     /gene="folC"
                     /locus_tag="ACHJ8C_001055"
     CDS             complement(24310..25578)
                     /gene="folC"
                     /locus_tag="ACHJ8C_001055"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO5550950.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWTFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25648..26562)
                     /gene="accD"
                     /locus_tag="ACHJ8C_001056"
     CDS             complement(25648..26562)
                     /gene="accD"
                     /locus_tag="ACHJ8C_001056"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO5550951.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26718..27377)
                     /gene="dedA"
                     /locus_tag="ACHJ8C_001057"
     CDS             complement(26718..27377)
                     /gene="dedA"
                     /locus_tag="ACHJ8C_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO5550952.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27460..28272)
                     /gene="truA"
                     /locus_tag="ACHJ8C_001058"
     CDS             complement(27460..28272)
                     /gene="truA"
                     /locus_tag="ACHJ8C_001058"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO5550953.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28272..29285)
                     /gene="usg"
                     /locus_tag="ACHJ8C_001059"
     CDS             complement(28272..29285)
                     /gene="usg"
                     /locus_tag="ACHJ8C_001059"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO5550954.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29351..30487)
                     /gene="pdxB"
                     /locus_tag="ACHJ8C_001060"
     CDS             complement(29351..30487)
                     /gene="pdxB"
                     /locus_tag="ACHJ8C_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO5550955.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLSEGQKLSVVLDVWEGEPELNVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30586..31581
                     /gene="flk"
                     /locus_tag="ACHJ8C_001061"
     CDS             30586..31581
                     /gene="flk"
                     /locus_tag="ACHJ8C_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO5550956.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTEYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSSQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNIATRPALWILLVAIILMLVWLVR"
     gene            complement(31578..32756)
                     /gene="yfcJ"
                     /locus_tag="ACHJ8C_001062"
     CDS             complement(31578..32756)
                     /gene="yfcJ"
                     /locus_tag="ACHJ8C_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5550957.1"
                     /translation="MTAVSQTETRSSANFSLFRIACAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVPAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRAPSH
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33021..34241)
                     /gene="fabB"
                     /locus_tag="ACHJ8C_001063"
     CDS             complement(33021..34241)
                     /gene="fabB"
                     /locus_tag="ACHJ8C_001063"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO5550958.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34400..36406
                     /gene="mnmC"
                     /locus_tag="ACHJ8C_001064"
     CDS             34400..36406
                     /gene="mnmC"
                     /locus_tag="ACHJ8C_001064"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO5550959.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRVHFISFEK
                     FPLTRADLALAHQHWPELAQWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TCQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIVS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDLLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELTVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLKLAQQQGLQIHYQYQLQDLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSDEDQQQNRQRLIDCFPHAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYDATLVEYASLAEKKDEAVSAPVYDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSEEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36527..36805)
                     /gene="yfcL"
                     /locus_tag="ACHJ8C_001065"
     CDS             complement(36527..36805)
                     /gene="yfcL"
                     /locus_tag="ACHJ8C_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO5550960.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWEHLFQQASQQ"
     gene            complement(36839..37387)
                     /gene="epmC"
                     /locus_tag="ACHJ8C_001066"
     CDS             complement(36839..37387)
                     /gene="epmC"
                     /locus_tag="ACHJ8C_001066"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO5550961.1"
                     /translation="MNSTHHYEQLIEIFNSCFSDDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37387..38196)
                     /gene="yfcA"
                     /locus_tag="ACHJ8C_001067"
     CDS             complement(37387..38196)
                     /gene="yfcA"
                     /locus_tag="ACHJ8C_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO5550962.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38196..39020)
                     /gene="mepA"
                     /locus_tag="ACHJ8C_001068"
     CDS             complement(38196..39020)
                     /gene="mepA"
                     /locus_tag="ACHJ8C_001068"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO5550963.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPALWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39024..40109)
                     /gene="aroC"
                     /locus_tag="ACHJ8C_001069"
     CDS             complement(39024..40109)
                     /gene="aroC"
                     /locus_tag="ACHJ8C_001069"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO5550964.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40144..41076)
                     /gene="prmB"
                     /locus_tag="ACHJ8C_001070"
     CDS             complement(40144..41076)
                     /gene="prmB"
                     /locus_tag="ACHJ8C_001070"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO5550965.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            41242..41793
                     /gene="smrB"
                     /locus_tag="ACHJ8C_001071"
     CDS             41242..41793
                     /gene="smrB"
                     /locus_tag="ACHJ8C_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO5550966.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42117..42974)
                     /locus_tag="ACHJ8C_001072"
     CDS             complement(42117..42974)
                     /locus_tag="ACHJ8C_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO5550967.1"
                     /translation="MKKTNLLFRLLILMVFMLGSRAGMGQTFRNVYIYYATFGWNSQV
                     ELYLDVAEVSSVYYGTYYQSNYVMTLGNLPNRTWTGTGSSPAPTITVKDGTIVNNSFC
                     PGMEALEAKDSRWECLKLPLDITVDGDVGGCPWLVSIRATSQVVVGSVDKDIYYGPNA
                     TVSVCPTVSVTPYDVSWDENYINKTKTLSLQSTGGVIEKTLSTYLMKDGKLCDGSQMN
                     EEGAYCRWVAQMITFSSSGCDNANVTVAPNRHPITDKQLHDMVVHVDTSSRRPINSTC
                     RFQYTLNEL"
     gene            complement(42976..43503)
                     /locus_tag="ACHJ8C_001073"
     CDS             complement(42976..43503)
                     /locus_tag="ACHJ8C_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5550968.1"
                     /translation="MNLTRTHYRYVALAAVLLMPQAQAADNLHFYGNLLSKSCTLVIQ
                     GESLVEVHFPTVSRKDLMVTGQSARVPVVFQLKDCKGPAEYEVRVTLTGTEDSEQPGF
                     LALDASSGAQGVGIGMEKTDGTAVPINSTTGATFVLSNGDNALNFNAWLQAKSGREVT
                     MGEFTASLTATFEYI"
     gene            complement(43500..43979)
                     /locus_tag="ACHJ8C_001074"
     CDS             complement(43500..43979)
                     /locus_tag="ACHJ8C_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5550969.1"
                     /translation="MKRLAWCLLYGFAGLSQAAVNDVTFHGTLVSPPACTISDGKSIE
                     VAFSNVIIDNINGDNFRQDVPYTITCDPDVRDDAWEMSLTWTGHQTSYDDAAIETDVA
                     GLGIELQQNGQPFKVGEQLTIDPASPPTLIAVPVKASDAALSEGNFSAWATLTVDYQ"
     gene            complement(43976..44479)
                     /locus_tag="ACHJ8C_001075"
     CDS             complement(43976..44479)
                     /locus_tag="ACHJ8C_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5550970.1"
                     /translation="MKAFFLAGSAALLLACGVTVSPAMAGGKIVNLTLRVLVDGPPPC
                     SIQGSAVEFGSVVIKGIDGTNYRQPVGYTMNCSNSVSDDLRMQLQATTTTINGETVLS
                     TGIDGFGIRIQNATDHSLVAVGNASWLPFNINTRPEFEAVPVKQSGVQLMASEFNATM
                     TMVVDYQ"
     gene            complement(44496..45248)
                     /locus_tag="ACHJ8C_001076"
     CDS             complement(44496..45248)
                     /locus_tag="ACHJ8C_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5550971.1"
                     /translation="MTYKLSCPMLVSTALMALLTTASLTAHASVTPDRTRLVFNESDK
                     SISVTLRNNNEKLPYLAQSWLEDEKGNKITSPLAVLPPVQRIDAMMNGQVKIQALPDI
                     HTLPSDRESLFYYNVREIPPKSGKANTLQIALQTRIKLFWRPKALEKIDMRKPWQFKV
                     TLTRSGQDYTVNNPTPYHVIISDASTQKKGLTAAGFKPLVMPPKTSQPLKAKLASAPV
                     LTYINDYGARMPLIFRCEGNTCKVDEDQSSKG"
     gene            complement(45268..46467)
                     /locus_tag="ACHJ8C_001077"
                     /pseudo
     CDS             complement(45268..46467)
                     /locus_tag="ACHJ8C_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305198.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT        11335 a        12647 c        11660 g        10931 t
ORIGIN      
        1 ttgcttatta agcatataca cctaattatt ttgccattat taagttatta acagcacaat
       61 cgagccatcc cctctggcaa aatcttattc tgcagacctt caaaacaccg tcctggggga
      121 gtacattgtt ctaagctgac ttccacggca gggagtggcg ataacagcaa aaaaggtcaa
      181 gattcatgtc ccccattgaa aaatccagca aattagagaa tgtctgttat gacatccgtg
      241 gtccggtgct gaaagaagca aaacgcctgg aagaagaagg taacaaggtt ctgaaactga
      301 acatcggcaa ccctgccccg ttcggttttg acgcgccaga tgaaattctc gttgacgtga
      361 tacgcaacct gcctaccgct caagggtatt gcgattccaa aggtctttac tccgcgcgta
      421 aagccatcat gcagcactat caggctcgtg gcatgcgtga tgttaccgtg gaagatattt
      481 acatcggcaa tggtgtatcg gagcttatcg ttcaggcaat gcaggcattg ctgaacagcg
      541 gggacgaaat gttggttcct gcacctgatt acccactatg gaccgcggcg gtttcgctct
      601 ccagcggtaa agcggtgcat tatctttgcg atgaatcctc tgactggttc ccggacctcg
      661 atgatattcg cgctaaaatt acgcctcgta cgcgtgggat cgttattatc aacccaaata
      721 acccaaccgg cgcggtatat tccaaagagc ttttaatgga gattgtggag attgcacgtc
      781 agcataatct cattatcttc gccgatgaaa tttatgacaa aattctctac gacgacgctg
      841 agcatcactc cattgcaccg ctggcacctg acctgctgac cattaccttt aacggactgt
      901 cgaaaacgta ccgcgttgca ggcttccgtc aggggtggat ggtgttgaac gggccgaaaa
      961 aacacgccaa aggttacatc gaaggtctgg aaatgctggc ttcaatgcgc ctgtgtgcta
     1021 acgttcctgc acaacacgcc attcagaccg cactgggtgg ttatcagagc atcagtgaat
     1081 ttattacccc tggcggtcgt ctttatgagc agcgtaaccg cgcgtgggaa ttgatcaacg
     1141 atattccggg cgtttcctgc gtgaaacctc gtggtgcgct gtatatgttc ccgaaaatcg
     1201 acgccaaacg ctttaacatt cacgacgatc agaaaatggt gctggatttc ctgttgcagg
     1261 aaaaagtcct gttggtgcaa gggacggcat tcaactggcc gtggccggat cacttccgca
     1321 ttgtcacgct accgcgtgtc gatgatatcg agctgtcttt gagcaagttc gcgcgtttcc
     1381 tttctggtta tcatcagctg taatcttaat ttcactgccg gagattgcat ccggcagtgt
     1441 tatcccgcca caatgacctg atgatgtcat catacgtaag gtcactatga aacagagcca
     1501 tttttttgcc catctctccc gcctgaaact cattaaccgc tggccgctaa tgcgcaacgt
     1561 gcggacggaa aatgtgtccg aacacagttt gcaggtagcg atggtcgccc atgcgctggc
     1621 agctatcaaa aatcgtaaat ttggcggtaa tgtcaacgcc gaacgtatcg ctttactggc
     1681 gatgtaccac gatgcttcag aagtgctcac cggagatctc cctactccgg tgaaatactt
     1741 caattcgcaa atcgctcagg aatacaaggc tattgaaaaa atcgctcagc aaaaactggt
     1801 cgatatggtt ccggaagagc tgcaggatat ctttgcgccg ttaattgacg agcatgcata
     1861 tagcgatgaa gaaaaatcgc tggtgaaaca ggcagatgca ctgtgtgcat atctgaaatg
     1921 cctggaagaa cttgcggccg gaaataatga attcttgctg gcaaaaacgc gactggaagc
     1981 gacgcttgaa gcgcgccgca gccaggagat ggactacttc atggaagtat ttgttcccag
     2041 cttccatctt tcgctcgatg agattagcca ggattcaccg ctgtaagcag ccggagtctg
     2101 cgtcgcatca ggcaataagc accggatgcg acatcaggct cttgtcaaaa cggaaacagc
     2161 atcgggatca tcaccacaca aaccgccatc acgataatgg tgaacggtac ccccaactta
     2221 acaaagtcac tgaagctgta attccccgga cctaaaacca gtgtgttaac cggtgaagaa
     2281 accggcgtca taaaggcggc ggatgctgcc atcgccacga ccatcgcaaa tggataaggc
     2341 gacacaccca tcgttttggc agcagccagc gcaatcggag ccatcaacac cgccgtcgcg
     2401 gtattagaga taaatagccc aataaccgcc gacaagacaa acaaacaacc cagcatcata
     2461 tgtggcccgt aaccgccgcc aatgtccatc agccctttca ccgccagcgc gacacctccc
     2521 gttttctgta atgccacagc aaatggcatc atcccaacga tcaaaataat gctcggccag
     2581 tgaatggatt tataggcgct ttcagcatct atacagcgga atttccccat cagcaggcag
     2641 gcgataatag cggcaacagg attaggaatt tcatcggtca gcattaacgc caccattagc
     2701 accagacaga aaatcgcatg gggtgcctgg ctgtgcgcgg gcgatgcttc actcacctct
     2761 tccggtaagt tcagcgctac gaagtcgcgg ccctgtttgg ccagcatacc gatcagtttc
     2821 cagttaccca caaccaggat gatatcgccc agcagcagag gctcatccgc cagcgaacct
     2881 tccagcgcca cgccattgcg ctttagcccc actacattca atccgtagcg ggtacgaaaa
     2941 ccaatttcgc gcaccgattt accaataagt tctgactcag gaattaatga aatctctgcc
     3001 atgcccacat caagggcctg gtcagaaaaa tactcgccgc gcagtaccat cggctccagc
     3061 aattgctcac tacaaaattg ccggagatcg acatcagccg cagacatatc aataagcaaa
     3121 acgtcacgcg cgcgaaattc agaaacccca ttaacgttca cgataacgcg acgaaaacgc
     3181 cgccagcgtt caacaccgat gacgttagca ccataacgtt cacgtaattt gagatcgtcc
     3241 agtcgttgac caatcattgg cgatccgggg cgaatagcca gacgtcgcgc acgcccggtc
     3301 agtcgatatt cacggataag atcgcgaaag gttcggcgcg tccagccttc gcgctgcggg
     3361 gtctgggtat cccctttcag catgaaacgc atcactaaca tatacaaaat acccagcacc
     3421 agcacgacca ggccaatagg tgttacgcta aagaaactaa aaccatgata gccttcacgc
     3481 agcaattcac tgttgactac caggttcggc ggcgtcgcca ccagcgtcat catgccgctt
     3541 atcagcccgg caaaacttaa cggcatcatc agacgcgacg gcgacgtttg catacgcatg
     3601 gcaacgctta acaccacggg aataaagata gcgacaacgc cggttgaact cataaacgcg
     3661 ccaagccccg cgacggtcag catcaacaaa accaacattt tgatttcact gttgcccgcc
     3721 actttgacca gccatgtccc cattacagtg gcaacaccgg tacggaccaa accatcgcca
     3781 ataatgaaca aggcggcaat caggacaacg ttaggatcgg aaaagccgga aaatacttcg
     3841 gggaccgtca gcgttccgct taatgcaaac gcgacaataa caaacaaagc gaccgcatcc
     3901 atacgcactc tgcccgtcgc aaacaagacg atggcaacgg ccagtaatga aagaacccaa
     3961 atcaattcac cgttcacaac ttatccttgt taattgaggg ggataacttg attctgccat
     4021 aaaaaagccc cgacgagacg gggctaaatc atgatcaggt gtttcactga ataataacat
     4081 cgccatttgg ctgtttggtc acagtaattt gctccagact gtggaggact aaatcgactt
     4141 cattcaggcg cggggtatct gccggggcat taaccgcaat aacatggcaa cccgccgcca
     4201 gacctgaaag cacgccagcg ggagcatctt ccaccacaac acactcctgc ggcgcaagcc
     4261 ccagcagctg cgcgcctaac agatacgcat caggttctgg ttttccgcgc ttcacccgct
     4321 cagcggttac aaacacctct ggcgcgggaa gccccgctat tttatgacgc gctcgcgcta
     4381 ccggcatgga gccagaagtc acaatagccc acggaatacc tgctttattc aaatgattga
     4441 gtaaggcgat tgcccccgga agcgcggtaa taccttcggt ttccgtggcc tcgatgtgct
     4501 ccagacgcgt aaactcggcg gcaatgtctg cctcggattt gcccgccata aaatggcgca
     4561 gagaggtgat cgcctgttta ccgtgaatga aagccagcac ctcttccggc gataacccat
     4621 gacgtctggc ccagttgctc cacgcccgtt ctaccgcagg cagggaatcc accagcgttc
     4681 catcaagatc aaacagaaaa cctttgcacc gcacgcgggc ctcctcaggc gttaataatt
     4741 tgattaattt cgttggcgct caaatggtac tgacgcgggc aggcatgcca gacattaagc
     4801 atgcgctgat atttttccca cattggcgtc tgggcgttaa aaccgtgagt tccagcgtca
     4861 aaatgggtat agcgcccttc cacattaacc ataaagcgga cataaccgag gtaacgtgct
     4921 tcagtggcgg cgtcaaagcc gaggaaggtg acacgacgtt catcgatgga ttgctggtcc
     4981 tgcaaattag accaggaaac atgcaacgca tgatacatct ccataatgtc gatgatagtg
     5041 cggcaggttt cttctttcag ctcgccaaac tcgcgatcca gttcacgcat ctgtaatcca
     5101 taaccacgct caatgattgt ttgcaggcga cggtaacgtt cagcatttgc cggatcgagc
     5161 atagtcatca tcttgtactg gttagacaaa ataagacgtt gcgcgttggt catttccatt
     5221 attgactcct gtatcactct actacggtga aaaaaagaag gctgagtatg ccttctttta
     5281 tatgcgtaat caggggtcaa ttacaaatca tcaaggaaag ttttatccag ttgtttgaag
     5341 gcacgcttaa gcgtgtcagc taatgcctgg taatcaggct tgccttcaac gggtgccaac
     5401 acctgtccag actcctgcaa tttaccgcga acttcataaa accagttgag gattgcagga
     5461 ggtaatggcg ttacagaacg cttgcccagc caccacaatc cctgcatggg taaacttaag
     5521 gcgaacagcg cagtggcaac tgccggccca agctgaccgc ccagagcaat ctgccagcag
     5581 agagtaaata cggcgatcgg cggcataaaa cggatcgcat aacgcgtcat cttgataacg
     5641 cgattttcga caaagactgg ggcaaggcgt ttttccagcg gccacgtctt tgagtaatgc
     5701 tgtccccggc gaaacaagct aaaaaaatta acagaacgat tatccggcgt tgacatgctt
     5761 cacctcaact tcacatataa agattcaaaa atttgtgcaa attcacaact cagcgggaca
     5821 acgttcaaaa cattttgtct tccataccca ctatcaggta tcctttagca gcctgaaggc
     5881 ctaagtagta catattcatt gagtcgtcaa attcatatac attatgccat tggctgaaaa
     5941 ttacgcaaaa tggcatagac tcaagatatt tcttccatca tgcaaaaaaa atttgcagtg
     6001 catgatgtta atcataaatg tcggtgtcat catgcgctac gctctatggc tccctgacgt
     6061 ttttttagcc acgtatcaat tataggtact tccatgtcga gtaagttagt actggttctg
     6121 aactgcggta gttcttcact gaaatttgcc atcatcgatg cagtaaatgg tgaagagtac
     6181 ctttctggtt tagccgaatg tttccacctg cccgaagcac gtatcaaatg gaaaatggac
     6241 ggcaataaac aggaagcggc tttaggtgca ggcgccgctc acagcgaagc gctcaacttt
     6301 atcgttaata ctattctggc acaaaaacca gaactgtctg cgcagctgac tgctatcggt
     6361 caccgtatcg tacacggcgg cgaaaagtat accagctccg tagtgatcga tgagtctgtt
     6421 attcagggta tcaaagatgc agcttctttt gcaccgctgc acaacccggc tcacctgatc
     6481 ggtatcgaag aagctctgaa atctttccca cagctgaaag acaaaaacgt tgctgtattt
     6541 gacaccgcgt tccaccagac tatgccggaa gagtcttacc tctacgccct gccgtacaac
     6601 ctgtacaaag agcacggcat ccgtcgttac ggcgcgcacg gcaccagcca cttctatgta
     6661 acccaggaag cggcaaaaat gctgaacaaa ccggtagaag aactgaacat catcacctgc
     6721 cacctgggca acggtggttc cgtttctgct atccgcaacg gtaaatgcgt tgacacctct
     6781 atgggcctga ccccgctgga aggtctggtc atgggtaccc gttctggtga tatcgatccg
     6841 gcgatcatct tccacctgca cgacaccctg ggcatgagcg ttgacgcaat caacaaactg
     6901 ctgaccaaag agtctggcct gctgggtctg accgaagtga ccagcgactg ccgctatgtt
     6961 gaagacaact acgcgacgaa agaagacgcg aagcgcgcaa tggacgttta ctgccaccgc
     7021 ctggccaaat acatcggcgc ctacactgcg ctgatggatg gtcgtctgga cgctgttgta
     7081 ttcaccggtg gtatcggtga aaatgccgcg atggttcgtg aactgtctct gggcaaactg
     7141 ggcgtgctgg gctttgaagt tgatcataaa cgcaacctgg ctgcacgttt cggcaaatct
     7201 ggtttcatca acaaagaagg tacccgtcct gcggtggtta tcccaaccaa cgaagaactg
     7261 gttatcgcgc aagacgcgag ccgcctgact gcctgatttc acaccgccag ctcagctggc
     7321 ggtgctgttt tgtaacccgc caaatcggcg gtaacgaaag aggataaacc gtgtcccgta
     7381 ttattatgct gatccctacc ggaaccagcg tcggtctgac cagcgtcagc cttggcgtga
     7441 tccgtgcaat ggaacgcaaa ggcgttcgtc tgagcgtttt caaacctatc gctcagccgc
     7501 gtaccggtgg cgatgcgccc gatcagacta cgactatcgt gcgtgcgaac tcttccacca
     7561 cgacggccgc tgaaccgctg aaaatgagct acgttgaagg tctgctttcc agcaatcaga
     7621 aagatgtgct gatggaagag atcatcgcga actaccacgc taacaccaaa gacgctgaag
     7681 tcgttctggt ggaaggtctg gtcccgacac gtaagcacca gtttgcccag tctctgaact
     7741 acgaaatcgc caaaacgctg aacgcagaaa tcgtcttcgt tatgtctcag ggcactgata
     7801 ctccggaaca gttgaaagag cgtatcgaac tgactcgcaa cagcttcggc ggtgcaaaaa
     7861 acaccaatat taccggcgtt atcgttaaca aactgaacgc tccggttgat gagcagggtc
     7921 gtacccgtcc ggatctgtcc gagatttttg acgactccac caaagcaaaa gtgaacaacg
     7981 ttgatccggc gaagctgcaa gaatccagcc cgctgccggt tctcggcgct gtgccgtgga
     8041 gctttgacct gatcgcgact cgtgcgatcg atatggctcg ccacctgaat gcgaccatca
     8101 tcaacgaagg cgacatcaat actcgccgcg ttaaatccgt cactttctgc gcacgcagca
     8161 ttccgcacat gctggagcac ttccgtgccg gttctctgct ggtgacttcc gcagaccgcc
     8221 ctgacgtgct ggttgccgct tgcctggctg ccatgaacgg cgtagaaatc ggtgccctgc
     8281 tgctgactgg cggctacgaa atggacgcgc gcatttctaa actgtgcgaa cgtgctttcg
     8341 ctaccggcct gccggtattt atggtgaaca ccaacacctg gcagacttct ctgagcctgc
     8401 agagcttcaa cctggaagtt ccggttgacg atcacgagcg tatcgagaaa gttcaggaat
     8461 acgttgctaa ctacatcaac gctgactgga tcgattctct gactgccact tctgagcgca
     8521 gccgtcgtct gtctccgcca gcgttccgtt atcagctgac tgaacttgcg cgcaaagcgg
     8581 gcaaacgtat cgttctgccg gaaggtgacg aaccgcgtac cgttaaagca gccgctatct
     8641 gtgctgaacg tggtatcgca acttgcgtac tgctgggtaa tccagcagag atcaaccgtg
     8701 ttgcagcctc tcagggtgta gaactgggtg caggcattga aatcgttgat ccagaagtgg
     8761 ttcgcgaaaa ctatgttggt cgtctggtcg aactgcgtaa gaacaaaggc atgaccgaaa
     8821 ccgttgcccg cgaacagctg gaagacaacg tggttctcgg tacgctgatg ctggaacaag
     8881 atgaagttga tggtctggtt tccggtgctg ttcacaccac cgcaaacacc atccgtccgc
     8941 cgctgcagct gatcaaaact gcaccgggca gctccctggt atcttccgtg ttcttcatgc
     9001 tgttgccgga acaggtttac gtttacggtg actgtgcgat caacccggat ccgaccgcag
     9061 aacagctggc agaaatcgcg attcagtccg ctgattccgc tgcggccttc ggtatcgaac
     9121 cgcgcgttgc tatgctctcc tactccaccg gtacttctgg tgctggtagc gacgtagaaa
     9181 aagttcgcga agcaactcgt ctggcgcagg aaaaacgtcc tgatctgatg atcgacggtc
     9241 cactgcagta cgacgctgcg gtaatggctg acgttgcgaa atccaaagca ccgaactctc
     9301 cggttgcagg tcgcgctacc gtgttcatct tcccggatct gaacaccggt aacaccacct
     9361 acaaagcggt acagcgttct gctgacctga tctctatcgg accgatgctg cagggtatgc
     9421 gcaagccggt taacgacctg tcccgtggcg cactggttga tgatatcgtc tacaccatcg
     9481 cgctgactgc gattcagtct gcacagcagc agtaatctcg tcatcatccg cagctttacg
     9541 ctgcggatat ctgaaccgga aatagtcact atttccggtt ttttattctc ttaatttgca
     9601 ttaatccttt ctgattatct tgcttaactg cgctgcatca atgaattgcg ccatctcact
     9661 ttgcatactt accactttgt tttgtgcaag ggaatatttg cgctatgtcc gcaatcactg
     9721 aatctaaacc aacaagaaga tgggcaatgc ccgatacgtt ggtgattatc ttttttgttg
     9781 ccattttaac cagccttgcc acctgggtag ttccggtggg catgtttgac agtcaggaag
     9841 tgcagtatca ggttgatggt caaacaaaaa cacgcaaagt cgtagatcca cactcatttc
     9901 gcattctgac taacgaagca ggcgaacctc agtatcaccg cgtacagctg ttcacgacgg
     9961 gcgatgaacg cccgggtctg atgaacttcc cgtttgaagg gttaacctca ggatcgaaat
    10021 acgggacagc cgttggcatc atcatgttta tgctggtgat tggcggcgcg tttggcattg
    10081 tgatgcgtac aggaaccatt gataacggta tcctggcgct tattcgccat acccgcggaa
    10141 atgaaattct ctttattcct gcgctgttta ttctgttttc acttggcggc gcaatatttg
    10201 gtatgggaga agaggccgtc gcctttgcca ttatcatcgc accgctaatg gtccggctgg
    10261 gctatgacag tattaccacc gtccttgtga cctatattgc cacgcaaatc ggttttgcca
    10321 gttcgtggat gaacccgttt tgtgtggttg ttgctcaggg tattgccggc gttccggtgc
    10381 tttctggctc cgggttgcgc atcgtggtgt gggttatcgc cactctgatt ggcctgatct
    10441 ttaccatggt gtacgcctca cgagtgaaaa agaatcctct tctgtcacgc gtgcatgagt
    10501 ccgaccgctt ctttcgtgaa aagcaggctg atgttgaaca acgtccgttt acctttggtg
    10561 actggctggt attgattgtc ctgaccgccg taatggtctg ggttatttgg ggcgtgatcg
    10621 ttaatgcctg gtttattcct gaaattgcca gccagttctt caccatgggt ctggtgattg
    10681 gcatcatcgg cgttgttttc cgccttaacg gcatgacggt taataccatg gcttcatcct
    10741 ttaccgaagg ggcgcgaatg atgatcgccc ctgccctgct ggtgggtttc gccaaaggga
    10801 ttttgctgct ggtcggtaat ggtgaagcgg gtgatgccag cgtgttaaat accatcctca
    10861 acagcattgc caatgccatt agcggtctgg ataacgcagt cgcggcctgg tttatgttgc
    10921 tcttccaggc ggtatttaat ttcttcgtga cgtccggttc tggtcaggcg gcgttaacca
    10981 tgccgttact ggcaccgctt ggcgatctgg tcggtgttaa ccgtcaggtt accgtgctgg
    11041 ctttccagtt tggcgatggc ttcagccaca tcatttaccc aacctcagct tcgttaatgg
    11101 caacgctcgg tgtttgccgg gtggacttcc gtaactggct gaaggtgggt gcgaccctgc
    11161 ttggactgct gtttattatg tccagcgtcg tggtgatcgg cgctcagttg atgggctacc
    11221 actaaaaatg ttaagagccg caattgcggc tctttttttc attctgccgt ttcagtctct
    11281 acggcttcat ttttggcatt gcgcttcatc cacaacgcca gcgctttcag cgagtctgga
    11341 gtgaactcat cgcagcgtgc ggtgatttct tccggcgtca gccagcaaac ttcactgact
    11401 tcatcttcct gtagagcgaa gggaccgtga gagacgcagc tgaacaacgc gccccagaca
    11461 cggcaatttt tatcttcgaa atagaactgc ccgtgctcgg caaaggggac cccggcaatg
    11521 cccaactctt cttccgcttc gcgacgcgcg gattccagca gttgctcatc ggcctggact
    11581 acaccgcctg cggtcgcatc taacatgccg ggtaaaaagt cttttgtctc ggtacgacgc
    11641 tggaccagaa ttttgcccat gccatcatgc acgacgatat aagttgcacg atgacgcaga
    11701 cactgtgccc gcatttgttc ccggctggct tgtgcaatga cttcgttctc ttcattgaca
    11761 atatccaccc attcagtact tgccaaacga cgctgttcca ttatcaggaa accttctttt
    11821 tctggcgctc ttacggcgca ttttgagttg tgggtaaatt acggattaat cgcgacctgc
    11881 gcaatgatac tttgatcatt gagtgcgatt acactaagta cgtcattatc cagcatgcca
    11941 taactcgccg gattaccgcc tttcggaata ctcaccgagc cggggttgaa gtggaaaatt
    12001 tccccccgct gttctgccac tggcagatgg gtatgaccgt acaccagcac atcgttctgg
    12061 tttaaagcag gtagattttc cgggccaaaa agatgaccat gcgtcaaaaa cagacgttgt
    12121 ttttccagta atacctgttg ccacggcgcg gttatcggga agttcagcag catttgatcc
    12181 acttcgctgt cgcagttgcc gcgcacagcg ataaccttat gtgccacttc attaagccgt
    12241 tcagcgactt tggctggcgc gtaaccctcc ggtaaagcat tacgcggacc atgattcaac
    12301 acgtcgccaa ggatcaccag ccactgggca ccgctttggg caaacaactc cagaacacgt
    12361 tccgtcgccg gtaacgaccc atgaatgtcc gatgcaaaca tcagtttcat cactcactcc
    12421 tcgtcgaaaa aacgtaagct atgatactgg attctgtcgc tgctatcagc cagattgctt
    12481 cgccgaaagt gcaataaaac gctggacaga cgctcgctgc cactggaatg tcgcataatc
    12541 caccaggcgt tccggcacct catcgccatg tagtaccagg cggttaatca ttagcgccag
    12601 atcagtatca gcaatgcacc attcaccaaa taaattcggc tgacccagtg ctaacaaatg
    12661 ttctgccatc gcgaacagtt tctctgcact ggcttttccc tcggccgtta gtggcgcttt
    12721 tttcgccccc gcaaagacaa catccgtcgg gcgttcttcg cgaatgggca tcagatcgct
    12781 gcgcagccag gcctgaatct gtcgtgcacg cgcacgattt tctaaatcaa gcggataaat
    12841 acgttcccag gtcggtggcg caaatcgatc ttccagatac tccgcaatgg cagaagattc
    12901 actcaactca aaatcatcga tttgtaataa cggcacacgg cgtgtctgac cgtaaccttg
    12961 ccacgtcggc tgcaaatgtt caccgctgtc gaggtcgatg gtcttgatat gaaacgacag
    13021 gcctttctcc tgcaacgcca cccaggcgga taacacataa ggggagaaaa aatgggcatc
    13081 tgaccaaagc gtgatagcgg gtttactcat tatgtcctcg gcaataaagg gggcttgcta
    13141 tcaaactata gagccttttt gtcactgtca cgtgacgaat actcacgcaa gtcgccgcgc
    13201 tttctatact tgtggtgttc agatgattag acaaccggag ctgcaatgat cgatctctat
    13261 ttcgccccga cacccaatgg tcacaaaatt acgctgtttc tcgaagaagc agggctggat
    13321 tatcgcttaa taaaggtaga cctggggaaa ggaggtcagt ttcgcccgga atttttgcgc
    13381 atttcgccta acaacaaaat tccggcaatt gttgatcatt ctccagccga tggcggcgag
    13441 ccgctaagcc tctttgagtc tggtgccatt ttgttgtatc tggctgagaa aacgggactc
    13501 tttttgagtc atgaaacgcg tgaacgcgcc gtcacattac agtggttatt ctggcaggta
    13561 ggcggactgg ggccgatgct tgggcaaaat catcatttta atcacgcagc cccccaaacc
    13621 attccttacg ctattgaacg ttatcaggtt gaaactcagc gtctgtacca tgtactgaac
    13681 aagcggctgg aaaattcgcc ctggctggga ggcgagaact acagcattgc ggatattgcc
    13741 tgctggccgt gggttaatgc ctggactcgc cagcgaattg acctcgcaat gtatccggca
    13801 gtcaagaact ggcatgagcg gatccgttcg cgccctgcca ccgggcaggc actgctaaaa
    13861 gcacaacacg gtgatgagcg ctcggatagt taacagaaac aggttctcgt gtattatttc
    13921 attctaagta aaacaacgga gaacctgcaa tggcacaacc tgccgctatt attcgtataa
    13981 agaaccttcg tttacgtacg tttatcggaa ttaaggaaga agaaattaac aaccgtcagg
    14041 atattgttat caatgtgacg atccactacc ccgccgataa agcgcgcact agcgaagata
    14101 tcaacgatgc gctgaattat cgcaccgtaa cgaaaaacat tattcagcat gtagagaata
    14161 accgtttctc tttgctggaa aaattaactc aggatgtgct cgatatcgca cgtgaacatc
    14221 actgggtgac gtatgctgaa gtggagatcg ataaactgca cgcgctgcgc tacgccgatt
    14281 cggtatccat gaccttaagc tggcagcgtt aaccgccata ttgggaggct gcatgaacat
    14341 agtgatcacc ggagggacgg gattaattgg tcgccatttg attccacgtt tgctggacct
    14401 gggccatcaa atcacggtag tgacgcgtaa cccgcagaaa gccagttccg ttctcggccc
    14461 tcgggtgaca ctatggcaag ggcttgccga acaaagcaac ctcaacggcg ttgatgcggt
    14521 aatcaacctg gccggagaac cgattgctga taaacgctgg actcacgagc aaaaagagcg
    14581 tctctgccaa agccgctgga atatcacgca aaaactggtc gatttgatta atgccagcga
    14641 cacgccaccg tcggtactca tttccggctc ggcaacgggc tattatggcg acttaggtga
    14701 agtggtggtt accgaagagg aaccgccgca taacgaattt acccataaac tctgcgcccg
    14761 ctgggaagag attgcctgtc gggcgcaaag tgacaaaacg cgagtgtgcc tgctgcgtac
    14821 cggcgtagtg ctggcaccag acggtggtat cctcggtaaa atgctgccgc catttcgttt
    14881 tggcctgggc gggccgattg gctctggtcg gcagtatctg gcctggattc atattgatga
    14941 tatggtcaac ggtattctct ggctgctgga taaagagctg cgcgggccat ttaatatggt
    15001 ttcgccctac ccggtgcgca atgaacaatt tgcccatgca ctgggtcatg cgctgcatcg
    15061 cccggccatt ttgcgcgtcc ctgcgaccgc cattcggttg ttaatgggcg aatcttcagt
    15121 tctggtatta ggcggtcaac gcgcgctgcc aaaaaagctg gaagaagcgg gttttgcgtt
    15181 tcgctggtac gatttagaag aggcgctggc ggatgtcgtt cgctgatgtg gtttactgca
    15241 aacatccgcc agttaactcc cggtgttaca gggttagtgg ctttgcgcga taagatcgtc
    15301 tggtgaaagt cgggtcacca tcagaactaa ctctcggtct aaacctctct ccagcatctc
    15361 ctgagcaata cgcagggctt cttcgcgttt gcccagaatt aatccatcat tccgcccttc
    15421 ttcacgtaat ctgtcagcaa tagtcatcag tttctccttt tcttgtggtg cgcgttccgc
    15481 tatctcacca ataaatgcac ggaaacgctg ggcatccccc gtttgtaata cgtaattaaa
    15541 cagggctttt agctgtctgt cattagtgtt ccctgtaact agcagcgaaa caatttggtc
    15601 gactaatccc aacagatcgc gctgacgaat atgtttctga attaactcca acagagccat
    15661 tttgcgatgt tgcataatct cgtcatccgg caccacggta atatccacca gcggaaaagc
    15721 ctatgaataa attttgcggg caatcgcagg ctcggcaaat tcatcaagcc agcagagtga
    15781 atatggataa gggcttctgc aaccatgata aaacagcatc gggatcacta atggaagctc
    15841 tttatagcct gcatcaagat ggttttgcat cgccgcaatg gaatagcgca tcatgcgaaa
    15901 agccattaat tcctctggct tactttggtg ctctatcact acataaatat aacccgctcc
    15961 ctcctgcgtt ttcacagacc acaagaggtc ggaataatat tgccgcaggt cttcatcaat
    16021 aaaactgttt ggttccagtt taagcgtcgt taaatcacac agtttgcgca gcggcgcggg
    16081 aagatgaata tcaataaaat cccgcgcggt gtctggatgg cgtaaaaaag atttaaatac
    16141 cgcatcatgc ggcgtggaag ttgtcgatat tgtcatggcg ttccgtcacc cctcaaattt
    16201 aagatgacgc gacaataacc acgatttaca cccaatgcag caggcagttt ttatctttgg
    16261 acagcgaatt cagaactatt tttagcggac tgcaactttt acatttcagc ggaagcggac
    16321 tcgcaaattc cccatgaaat actccggcag cgttaaccac cggagaggaa attatttcag
    16381 cgaaccttta aggaactgtt gcagacgaga gctttgcggg ttaccgaata actgctccgg
    16441 cgccccttct tcttctattt tcccctgatg gaggaaaatg acatgagtag aaacatgacg
    16501 agcaaagccc atttcgtgag tcactaccac catggttttc ccctcttctg ccagttgctg
    16561 cataatacgc aacacttcgc ctaccagttc aggatcgagc gctgaagtag gttcatcaaa
    16621 cagtaaaact tccggttcca tcgctagcgc ccgcgcgata gaaacacgct gttgctgacc
    16681 accggacagg tgcaccgggt atttcccctg cgcacgttcg tctatcccga cttttgccag
    16741 atacttcacc gcccgctcgc gcgcttcctg cttgctcagg cccaacacct gaatcggcgc
    16801 ttccatgacg ttttccagca ccgtcatatg gctccagaga ttgaagtgct ggaataccat
    16861 cgtcaggcgt gtgcgcaata agcgcagctg atttttatcg gcgactttga gttgaccgtc
    16921 tttatcgcgc accagattga tcgtctggcc attgaccacg atcgatcctt cgctcggttt
    16981 ttcgaggaag ttaatgcagc gcagaaaggt acttttaccc gatcccgacg atccgatgat
    17041 gcttatgaca tctccggcat tcgcttgcaa tgagacccct ttcagcactt cgtgttcgcc
    17101 gtagcgtttg tgcaaatcga taacgtttaa tttattctcg gacatcgtat tctcagtgcg
    17161 ttgaagaagg tttcacatgc tgcaaccagc gtttttcagc tctacgaaag aggctgatca
    17221 ggacataaga gatgattaaa tagagcaccg cggcaatgcc gaaagcggta aaaggttgat
    17281 atgtggcggc gttaatatcg cgggcaattt tcagcagatc cggcaccgtg gcagtaaatg
    17341 ccagcgcagt agagtgcagc atcaggatca cttcgttgct gtacgccggt aacgcaatac
    17401 gcagcgcaga aggcaaaata atgcagcgat acattttaaa ggtcgagaag ccataggctc
    17461 tggcggcttc aatttcccca tgcggcactg aacggattgc cccggcaaaa atctcggtag
    17521 tgtaagcgca ggtgttaagc gtcagcgcca gcacggtaca gttcagacca ctgcgaaaaa
    17581 aggcgttaag gaactcggtt cccttaacaa tctccagcgt gtacatgccg gaatagaaca
    17641 ccagcaactg aacatacagc ggcgtaccgc gaaaaatata ggtaaataac cagattggaa
    17701 actgaatgta tttattactt gagacgcgac caatcgccag aaacagcgcc agaactccgc
    17761 ctatcactac cgacaaaatc agcagccaca gggtgatcgc cacgccagta aagcgataac
    17821 cgtcagtcca cagcagtggt ttccagtatt catgtaagat ttcgatcaca ggtcagccct
    17881 cttcacaccc acggagtagc ggcgctcaag gaacagcagc acaccattgg aaacggtggt
    17941 gaaaacaaga taaatcacgc cgcagacgat agcgaaatag aacggttccc aggtactttt
    18001 gcctgccagc tgcgtggctt tgaccacatc ttccaggccg agtaacgaaa ccagtgcggt
    18061 agatttgagg atcacctgcc agttgttgcc aatgcctggc agggcgtaac gcatcatcgc
    18121 cggaaacatg atccgccgaa acacttgccc acgagtaaaa ccgaacgccg tcgccgcctc
    18181 tatatgtcct ttcggcactg ccataaacgc gccacgaaac gtttcggtaa aataagcacc
    18241 gtagataaaa ccgagagtga taatacctgc gaccatcgga tcaatatcaa tctgcccgac
    18301 gcccatcgcc tccgtcaccg tgtttagcgc aatctgcaaa ccgtagaaaa tcagcagcat
    18361 caatactaaa tccggcacgc ctcgaatcag cgtggtgtac ccttcgaaaa taaggcccga
    18421 aagccgattt tgtgagagct taccgccagc gccaattaaa ccgatgatta cagcgagcac
    18481 tacagagctg atagccagct ccagcgtgac gagcgcaccc tgtaaaataa cacttgaaaa
    18541 cccatacaac atgctgcctg tcctgtcgtg tgtggtgaat taccgcctct tgtctccctc
    18601 cggttacccg aagggagcgg gctttctttc accgatggcg attaaccacc ataaacatca
    18661 aaatcgaagt actttttcgc taatttctcg taagtaccgt cagcgcgcat ttcggcaaag
    18721 gctttgttca gtgcctcgcg cagttcgtta tcttctttac gcaggcccat gccggtccct
    18781 acgccaaaca gtttttcatc tttaacagac gggccaccga atttgtaatc tttaccgacg
    18841 ggttgtttga ggaaaccttc gctggctgcg acctcatcct ggaacgcggc atcaatacgt
    18901 ccggcagtca ggtcagaata aatattgtcc tgcccttgat aagaaacgat ttcaatgcct
    18961 tttggtgccc agtgttcgtt accgaacgtc tcctgggtgg tgccctgcaa tacgcctacc
    19021 cgtttgcctt tcagcgattc gactgtcggc tgaatgtcgg aatttttcgc caccaccaga
    19081 cgagaatcgg ctgcgtacag tttgtcagtg aaggctattt cttgctgacg tttttccgta
    19141 atggaaagcg atgacatgat ggcgtcaatc ttcttcgctt ttaaggacgg gattaacgca
    19201 tccagtgggt tttcgacaaa cgtacattgc gtattgatgc gtttgcacaa ctccttcgcc
    19261 agatcgatat cgaagccaac cagttcacct tgtgaatttt ttgattcaaa tggcgcatag
    19321 gtcggatcgg taccgatgcg gatgttttgc ggaatcgcag caaatgccgc agttgcgctg
    19381 gagaaggcca gaaccagaga gagcgatagc accagttttt tcataatgtc ctcaactgac
    19441 agtcttttta tcgggaatat ttacaggttt gttacactta tcgtgccata aaattgacca
    19501 tcaaggcaaa atatcccgcc ccgctagacg tattattttt cattaaaaat gcttaagtat
    19561 gcatttcaat gcaccacgat agtgcaacaa acctggcgtg caccatgatg atcattccat
    19621 caggtacagc ttccctgcga cgtatcagtc accgtagaca ttaaagtcga aatacttttt
    19681 cgccatcttg tcgtaggtgc cgtcctgacg cagctcgtta agcgccttat tgaaggccgc
    19741 cgtcagttca gcatcatctt tacgcagtcc tacaccggtg ccgtcaccga agtatttttt
    19801 gtctttcact gatgggcctg caaaggcaaa atctttaccg gcgggttgct taaggaaacc
    19861 ttcgctggca gcaacttcat cttgtaacgc agcatccaga cgtcctgcag ccagatcgga
    19921 atagactaaa tcctggttgg cataggccac cacatccacg cctttgctac gccaggtctc
    19981 gttagcgtaa gcttcctggg ttgagccctg cagcacacca acatgtttac ctttcagtga
    20041 atccagcgtt ggctgaatcg gtgaaccttt ggcggcaatc aaacgagaat ctgcggcgta
    20101 cagcttgtca gagaaggcta tctcctgctg acgtttatcg gtaatggaga gcgacgaaat
    20161 aatggcgtcg atttttttcg ctttcagtga ggggatcagc gcgtcaaagt cactggcaac
    20221 ccaggtacat ttcacctgca tccgtttgca catctcgtta ccgagatcga tatcaaagcc
    20281 aacaaagtca cctttggcat ctttcgatga gaacggtgcg taggtggtat cggttccgat
    20341 acgtaccgtc tccggtagcg ccgcatagct ggaagccgct gcggagagac cgactaacaa
    20401 agacagagcg agaatcgact tcttcataca taaccctcaa gtgaaaatgg cttttttatg
    20461 ttgtgtattg tgttgtgtgt ttgcaggctc tttcatgcag gtcttatgcc atcttgacga
    20521 cagcagtaac attcaacgtt aaatatgtta ataagacgtt gcattattgt cctaaagttg
    20581 aagatagcag gtatagcggt tgaatcgcag cgtttcgatt gaatggcaga aacaaaatgt
    20641 cgaggatttg atcgcggttg caaaattgcc ctgaaacagg gcaacagcgg agttatgcgc
    20701 cctgccagcg ggcaaagaga tcttcaggaa gggttaccgc aaactggtca agaacacgat
    20761 taaccgtctg atttatcaca tcatcaaggg attgtgggcg atgataaaac gccggaacgg
    20821 gaggcataat caccgcaccg atttctgccg cctgagtcat taaacgcaga tggcctaagt
    20881 gcagtggcgt ttcacgcacg cagagcacca acgggcgacg ctctttcagc accacatctg
    20941 ccgcacgggt cagtaagcca tcagtatagc tatggacaat gccggacagg gttttgattg
    21001 aacagggtaa aatcaccatc cccaacgtct ggaaagaacc ggaagaaatg ctggcagcaa
    21061 tatcgcgcgc atcgtgtgtc acatcagcta acgcctgcac ttcgcgcagg gaaaaatccg
    21121 tttcgaggga taaggtctgg cgcgctgcct ggctcattac cagatgcgtt tcgatatctg
    21181 tgacatcgcg cagaacctgt aataaacgca cgccataaat cgcgccgctg gcaccgctga
    21241 tgcctacaat gagtcgtttc ataaatagtt gccctgtcag acttgcaggc agactttgca
    21301 ggatttcggc gggagttgca agtcagggtg ccagaccggc accctgagcg aaggcatcat
    21361 ccttcgttat gcatttcgag attttccact tcgttctgac gttgcactgc tttggcgtcg
    21421 tcattacgta aagtatcgag gaaatcgagg tagccctgat caacgtcttt ggtgacgtag
    21481 acgccgttga acaccgagca ttcaaactgc tggatatccg ggttttcagc gcgaacggct
    21541 tcgatcagat cgttcagatc ctggaaaatc aacccgtcag caccgatgat ctggcgaatt
    21601 tcatctactt cgcgaccgtg agcgatcagt tccgtggcgc tcggcatatc gataccgtaa
    21661 acgttcggga agcgaatttc cggtgccgca gaagcgaggt acactttctt cgctccggct
    21721 tcgcgtgcca tctcgataat ctgctcagaa gtggtgccac ggacgataga gtcgtcgacc
    21781 agcaggacgt ttttatcgcg gaactcggcg cggttggcgt tcagtttacg gcgcacggac
    21841 ttacgacgca gctgctgacc cggcatgatg aaggtgcggc caacatagcg gtttttaacg
    21901 aagccctggc ggtacggttt gcccagaata cgcgcaattt ccagcgcgat atcacacgag
    21961 gtttccggaa tagggatcac cacgtcgata tccagatctt cccattcgcg ggcaattttc
    22021 tcgcccagtt tggtgcccat attcacgcgc gcgctgtaaa cggaaatttt gtcgatgaac
    22081 gagtccgggc gagcaaagta tacatactca aacaggcacg gattgctgac cggattgtca
    22141 gcacattgac gggtaaacaa ctgtccttct tcagtgatgt aaatcgcttc gcccggcgcg
    22201 acgtcacgca ggaaatcaaa gcccagcgta tcgagcgcta cgctttcgga agcgaccata
    22261 tattctgtgc ggttctcgtc aatatcacgt tttcccagta ccagcggacg aatcccgtta
    22321 ggatcgcgga aagcaaccat accgtggccg atgatcatcg ccacacaggc atacgcgccg
    22381 cggattaagc ggtttgtggc agcaatggca gcgaaaatgt tgtcggcttc cagcgggtaa
    22441 tggcggaagt tgtccagttc gcttgcgaaa atattgagca gaatttccga gtcggaagtg
    22501 gtgttgatgt ggcggcgttt ttcttcaaac agttttttac gcaactcgtg agcgttggtc
    22561 agattgccgt tgtgggcaag cgtaatgcca tacggagagt taacgtaaaa cggctgcgct
    22621 tcagaggcgc tggagctgcc cgccgtgggg taacgcacat gaccaatgcc catattgccc
    22681 tgcaaacgct gcatgtggcg agcttcaaat acatcgctca ccagcccgtt cgctttacgc
    22741 aaacggaagc agttattggc atctatggtg atgatgccgg cggcatcctg accgcgatgc
    22801 tgaagcaccg ttaaggcatc ataaatcgac tggttaaccg gcataacacc ggcgataccg
    22861 acaataccgc acatacgtct ttttcctcgt taagccacat ctcagagcac ttacgctctg
    22921 ggcaagaaac ttgacgagct ttgcagataa tcaaaaaacc atctgatgat aaaactgaac
    22981 tgcgggatca gctgtgattt gctccagtct tcgcttttcg acaccccggt aaaggagtcg
    23041 agaaagaaga gaatggcggc aacaatcaac acaccgcgca acgcaccgaa acagacgccc
    23101 agcacccgat cggtgcctga caaccccgtt ttctccacca actggcctat cacgaagttc
    23161 acgatagcac caacaatcag ggtagcgata aacagtaccg cgatggcaat cccatttcga
    23221 accagttcgt cttcaaagcc cgtaaaccag actgacaggt aagtgtagta atgactggca
    23281 acaaagaaag cacaacccca tgtcaccagc gataacgctt cacgaacaaa gccgcggatc
    23341 aggctaacca gagaggaaaa agcaatcacc gcgattatgg cgtaatcaat ccagaccata
    23401 tgtgtcccac gattttacgc cctgtcatcc tgttcggggc gcattctaac agaaaaagaa
    23461 aacgtttgcg tagggatttc cttcccgcgc atcaataaaa atggcgctga aaaaatattc
    23521 aacgccatcg actttttatg cctttgcggc atcgggcaat gcgtgtcgga tgccgcatga
    23581 acgccttatc cgacctactg ttctacccct gcacaggcct gataagatgc gccagcgtcg
    23641 catcaggcaa gaccgtatta attcggtgta tagcccatta ccacgccact taagccagaa
    23701 agctgcttga gctcacccag cgatcctttc agcttctctt tggaagcatc tggcccaacc
    23761 agaatacggg taattttacc ctgcactggc gtggatggcg acgtataaac ccgataaccg
    23821 gcaccgcgca gcttaccgac aatctcattc actttatcgg catttttcag cgcgcccagt
    23881 tgcacaacat aagctttacc cgttggcgct gctttttctt ccacgaccgg cttcggttct
    23941 ggcttcggcg caggtggtgc ttcaaccttt ggcttaagcg gctccaccgg tttcggcttc
    24001 ggtggggcga ccggtgcagg ttccggttca aactcggtgt tattggctgc aatagtggcc
    24061 ggatcgagcg acggtgctgc ggcatcacct gcccgtacct cttccgctgc gccttccggc
    24121 ggctgcgtcg gcaacgcctg ggtggcggct ggcatcatat caggctcatc gcgatcgccc
    24181 gcttttggca ccagcgggat agccgcgaac tcatcctgat aatgtttttt ctgcccgtcc
    24241 agcagccctg gaagtacaat cacccccagc gctaccagca cgatcgtgcc cactaaccga
    24301 ttctgaaact tacttgccac cgcttctcct cgcgtcaatc acttccatga catgtgcgac
    24361 cgtgtggaac gaaccacaca ccagcacggt gtcttccgct ttagcgtccg ccattgcggc
    24421 atcccatgcc tgcgcaacgc tatcaaatga tttgccgtta cccaaatgct caagcagttg
    24481 ttctgccgtg gcaccgcgcg gcccttccag tggcgcacaa taccagtcat caaccacgct
    24541 tttcaaccag gccagagttc cggcaatatc tttatcatgt agcataccga taaccgccag
    24601 cacgcgcccg tttttcggta gcgctttcat acgcccggtg agatattccg ccgcatgtgg
    24661 attatgcgcg acatcaaaaa taacgcgtgg cgactcgctc acaatctgga aacgtcccgg
    24721 caaaattgcg ctggcaatcc cgtcgcgaat ggcattttca ctgacctcca gcccgctggc
    24781 acgcagtgcc gccagcgccg ttgcggcatt cggttgcggg acaagcggca acggcagatt
    24841 ttccagcgtg ccgtgagcat cgctaaacgt ccagtcatga tcggtgacgg aatagttcca
    24901 ctcaacgccc cgacgttgta gcagtgcacc tttttcctgc gccacatcag caatggtaga
    24961 aggcatctcc ggctcaccga caattgccgg tttttcgctg cgaaagatac ctgctttctc
    25021 gcgaccaata ctttcgcgat ctggccccag ccagtcggta tgatccagcg caatactggt
    25081 tactaccgcg acatcggcat cgacaatatt ggttgcgtcc agacgaccgc ccagccctac
    25141 ttccagaatc actacgtcaa gttgtgcctg cttgaacagc cacaacgccg agagcgtacc
    25201 gtactcgaaa taggtcaggg aaatatcacc gcgtgccgat tcaatctccg caaaagaagc
    25261 ggtgtgggcc gattccggca attcctgacc ctgcacacgt acgcgctcgg tataacgcac
    25321 cagatgcggc gaactgtaga cgcccacttt gtaccctgcc gccatcagaa tcgactccag
    25381 cgtacggcag gtggtgcctt tgccattagt acccgcaacg gtaaacacaa atggcgctgg
    25441 tttcagaacg ccaagacgcg ccgcgacctg gctcacgcgc tcaaggccga gatcgatagt
    25501 tttactgtgc aggttttcca gataagaaag ccacgaagcc agaggcgacg cggcttgagg
    25561 agtgcgtttg ataatcatgg tatccgctga ttcgttacgg tgagaatagc aaaagggcag
    25621 agccagtggc cctgccctta tcagttatca ggcctcaggt tcctgatccg gtaccggtgg
    25681 caccactacg ccttcacgcg gcgcttcagg attcggcgct ggcagattca tcaacttcgc
    25741 cagaatgctc gccagtttca ggcgcatttc cggacgacgg acgatcatgt cgatcgcgcc
    25801 tttctcgatc aggaattcac tgcgctggaa tcctggcggc agtttttcgc gaacggtctg
    25861 ttcgataaca cgcggaccgg caaagccgat taacgctttc ggttcagcga tgttgagatc
    25921 gcccagcatg gcgaaacttg cagaaacacc gcccatggtc gggtcggtca gcacggagat
    25981 gtacggcaag ccgcgctcct gcatttttgc cagtgccgca gaggttttcg ccatctgcat
    26041 cagcgacatc agtgcttcct gcatacgtgc gccaccagag gcggagaagc agatcagtgg
    26101 gcagttatct tccagcgcct gctcaacggc acgcacgaaa cgcgcaccta ccacagaccc
    26161 catggaaccg cccataaagg cgaactcgaa tgccgcagcg acaaccggca ttccatacag
    26221 agtgcctttc atcaccacca gcgcatcttt ttcgccggtt tctttctgcg cagatgccag
    26281 acggtcttta tacttcttgg agtcacgaaa cttcagcaca tctttcggct caagctcgct
    26341 acccagctcc acaaggcttc cttcatctaa caggctatgc aggcgattac gcgctgtcat
    26401 acgcatgtga tggtcacact tcggacagac ctcaagatta cgttccagct cagcgcggta
    26461 taaaacctga ccgcagctat cacacttagt ccacacccct tcaggaatgc tcgccttgcg
    26521 ggtgggagta atgttgcttt taattcgttc aatccagctc attagggacc tttctgtctg
    26581 aacctggttc gatgccagtt ttatctttgg ggacgcataa tgccattttt gcccccaaca
    26641 gaccatgaat gttgcacatt aaaacataac agcccgaaac tttggataaa aaagtggtcg
    26701 aaccgcagag ttacttttta ttttgcggcg cgtgccgcag cgcgtttgtg gcgaataaat
    26761 tcgaaaacac ctggcaatat tgaaacaaaa ataatgccaa caatcatcag tttaaggttg
    26821 ctctgaatga aggggagcgt gccgaagaaa tagcctgcgt aggtaaaaag cagtacccac
    26881 aacagtgcgc caatcacgtt ataagcggcg aaatgacggt acgacatgtg gcccattccc
    26941 gcaacaaacg gggcgaacgt tctgacgatc ggcacaaaac gggcgagaat aatcgttttg
    27001 ccgccatgtt tctcataaaa ctgatgggtt ttgtcgagat aactgcgacg gaaaattttc
    27061 gaattgggat tactgaataa cttttcaccg aacaaccgcc cgatagtgta attgaccgcg
    27121 tcacccacaa tcgcggcgat cagcatcagc accaccatca tatggacgtt gagatcgttg
    27181 gtctccagtg atgccaacgc tccggcaaca aacagcaatg aatcacccgg taaaaacggc
    27241 gttaccacca gaccggtttc acagaacaaa attaagaaca aaatggcata aacccagacg
    27301 ccgtactccg cgaccagctc tgccagatgc acgtcaatgt gcaggataaa atcgatgagg
    27361 aaataaatca ggtccatatt tgcctatgcc ttgtactcgt catctatcag gctgtaacta
    27421 cgttggctac agcctgaaat gttccgagca ttattctcgt tagtccgcca gaaatagcgg
    27481 gcccattggc ggttttggaa gatcatatcg gtcagggtaa tccaccgcga ccagatatag
    27541 cccttccgct tttgccgttg ctgccgccag cgttctgtcc tttgccgcca gcaactctgc
    27601 tatccagctc tccggctggt tgtgggcacc gacttccatc aggctgccga caatattcct
    27661 gaccatatga tgtacaaagg cattcgcttt gatatctacc accacataag gaccgtgacg
    27721 cgtgacatta atatgcataa cgttgcgcca cggggtccgg gactgacact gcaccgcacg
    27781 gaacgaggtg aaatcattct cgcccagcaa acattgcgca gcccgatgca tccgttcagc
    27841 gtccagcggt tcgtaaaaat gggttacccc tttactcaat accgccgggc gcagccgatg
    27901 attgtagatg atgtagcgat aacggcgagc cgtggcgcta aatcgggcat gaaaatcatc
    27961 aggtacagct ttaacccaac gcacggcgat gtcaccaggt aaattcgcat ttacgcctaa
    28021 cgtccacgca gcgtctttgc gctgcgcggt cgtttcgaaa tgcaccacct gcccggtacc
    28081 gtgtacccct gcgtcagtac gcccggcgca gaagacggtg atgggttcgt tcgccacctg
    28141 ggagagcgcc ttttccagct tctcctgcac actgcggact tcattctgcc gttgccagcc
    28201 gtaataccta ctgccgtcgt actcaatgcc cagcgcaatt ttataaactg gcggttgttg
    28261 ctggtcggac attagtacag atactcctgc accagtttct cggcaatttt tactgccatc
    28321 agcgcgccgc caaagcgaac gttatcggcc accgaccaga actggacttg ctccggcata
    28381 ccgtagtcat tacgtacgca gccaacagaa agatgcggcg taccagaagc atcccctacc
    28441 tgagtcggga attcgttctc ttcagagagc acaatatctt cgccctgagc aaacgcatca
    28501 cgcgcttctt ctgccgccag cggacgcagt gcttcaaagt tgaccatctg ggcatgaccg
    28561 tagaataccg gtgcctggac gacgcttgcc gaaatcatca gcccttcgtc ctgcaggatt
    28621 ttgcgtactt cgtcaacgat acgacgttct tcacgcacgc taccttcgct atccggcagt
    28681 aacggcagca tgttgaacgc cagctgacgc ccgaagaaat cttcttcgtc aatcggaatg
    28741 ccattgagta atttagcact ctgccccgct aacgcatcga ccgctttttt gccctgggcg
    28801 gaggctgaaa tcaggctggt aacgctgata cgtgataaac cgccctgatc aattagcggt
    28861 ttcagtgccg ccagcagctg gctggtcaga ctgtctggta cggcgatgac attccggttg
    28921 cggtaatcgg tcagtacaaa cgggtttact tccggcacca ccagcggtac gtcgggttcg
    28981 agagcaaaca atccgctgct gtcgatcacc agacaacctg agttggtcgc ttcttcaacc
    29041 caggcagcgg tagcctcttt gcctgcgaca aaaaacgcca gctgcgcctg cgtccagtcg
    29101 aattcagcgg catcctgcac ggtgattgtc ttaccaccaa agcgcagttg ttctcctgcg
    29161 ctttcgttac gtgccagtgc ataaatttcc ccaaccggga actgacgttc agccagcgtt
    29221 tcaagcaggg cttcgcccac agcgccagtt gcgcccagga cggcaatgtt ccagccttca
    29281 gacatggtgg tttactccag aaatagcaaa gctccctgcc aatgttacag cagagagcat
    29341 gaagaagaga ttaacgtgcc ggatgatgaa cggcgttaaa acccagttta cacagcaatg
    29401 atgccgcact ggcgtcatca caaatcacat acagagatga ccattcacgg cgctcaagat
    29461 agtttttacg cagtttatcg aactcacccg gtatcccggc gactttacgc agcggtgcgt
    29521 catcgcggcg cacatcatac accaaatgca ccagcctttt cagcgtcggt tgatcgagcg
    29581 ggccatgcag cgtaatgcga ccaaactctg gcgcgggcag taatgtatcc agcgcaacgt
    29641 gctgttcatg cccaataaac ttgctataag cttcaaacac ttgcgtagta ccgcgtgctt
    29701 taccttccag ggtatagcct gcgatatgcg gcgtgccgat atccactttt ttcagcaatt
    29761 ccacgttaag ttccggttcg ccttcccaga catccagcac tacgcttaac ttctggcctt
    29821 cgctcaggca ggtcagcagg gcggtattat cgacgactgc gccacggcag gcgttaatca
    29881 ggatcgctcc cggtttcagg ctgcggatca gtttttcatc cgccagatgc agcgttttgt
    29941 acggaccatc tttaaagagt ggcgtatgga aagtcagaat atccgcatgc tggactaact
    30001 catccagcga gcggaaatcc ccctcatccc cacggtcggc gcggggcgga tcgcaaagta
    30061 aggttttgat ccctaacgct tccagtcgcg cctgtaaacg acgaccaacg ttaccaacgc
    30121 caacgatccc cacggtacgc tcgtgcagtg aaaatccatc gcgttcggca agcatcagca
    30181 gggaggagaa aacatattcc actaccgcaa tcgcattaca gccaggtgca gcggaaaaac
    30241 caattcccgc ctgctttaac catgcttcat cgacatggtc ggtccccgcg gtggcagtgc
    30301 caacaaattt aatgggtttt cctgccagca aagattcatt cactttcgtg accgaacgca
    30361 ccataagcgc atccgcgtct gccagttgag cgacaggaat tggacgcccg ggaaccgcgg
    30421 tcacctcacc caaacggcta aataagtcgc gggcataagg catattttca tcaacaagga
    30481 ttttcacgtt tgtgttacct gtatgagacg agagttaacc agacaagtgt gccataatct
    30541 cgcggccagg catacttgcg aagatttcag gtataaggat acgtaatgat acaacctatt
    30601 tccggccctc ctcctgggca accaccaggt cagggagata acctgccgtc tggcgcgggc
    30661 aatcagcctt tatccagtca gcaacgtact tcgctggaaa gcttaatgac gaaagtgacc
    30721 tcactgacgc aacaacaaag agcagaactg tgggcgggta tcaggcacga tattggtcta
    30781 tcgggagatt caccgctgct ttcgcgtcac ttccctgccg ctgagcataa tctggcgcaa
    30841 cgtctgttgg ccgcgcaaaa aagccattct gcccgccagc ttttagcgca attaggggag
    30901 tatttacgtc tggggaataa tcgtcaggcg gtcacggagt atatccgtca taactttggt
    30961 cagacgccgc tgaatcagct ctcaccggag caattaaaaa ccattctcac cctgttgcag
    31021 gaagggaaaa tggttattcc gcaaccacag cagcgcgagg caaccgaccg tcctttatta
    31081 ccggcggagc acaatgcgct caaacagctg gtgaccaaac ttgcggcggc aacgggggaa
    31141 cccagcaaac agatctggca atcgatgctg gaactttccg gggtgaaaga tggcgagtta
    31201 attccagcga aactgtttaa ccatctggtg acctggctac aggcgcgtca gacgctaagc
    31261 cagcaaaata cgccgacact ggaatcacta cagatggcgc taaaacaacc tttagatgcc
    31321 agtgaactgg cggcgttatc ggcatatatc cagcaaaaat atggcctttc tgcgcaatca
    31381 tcgctttctt cttcccaggc cgaggatatt cttaaccagc tttatcaacg gcgggttaaa
    31441 gggattgatc cgcgtgatat gcaaccgctg cttaatcctt ttccaccgat gatggacacg
    31501 ttgcaaaata tagcaacgcg tcccgcgctg tggatactgt tagtcgcgat tatcctgatg
    31561 ctggtctggc tggttcgtta accccgacga aacgacagta tcgtgacaat aattcccagc
    31621 accgcagaga tcgccccggc aagaaatacc gaagagtaac caaacgtggt cgccagcatt
    31681 cccgcaagcg gcccggagac gccgagggcg atatcctgaa acgcggcgta accgcccagt
    31741 gcggtgccgc gaacgtgtga gggggcgcgt ttaaccacct ccacgcccag cgcaggaaag
    31801 ataagcgaac atccggctcc ggttaacgca gcacccgcta atgcgaccca cgcccctggg
    31861 gcttgccaga gcagcaacaa gcccaccgtt tctacaagca gagagacgat cgccactttc
    31921 acgccgccaa aacggtccgg catccagcca aacatgacgc gcatcacgac aaatgcgccg
    31981 ccaaacgcgg taagagtaaa gcccgccatc gcccatcctt tgctggcaaa gtagagcgaa
    32041 acgaaagtcc cgataaccgc aaaaccaacg ccttgtagtg ccagacctaa tcctggtttc
    32101 cagataagcc cgacaacgct ccacagcgat ggacgttctc ccgccagggc cggtactttg
    32161 cgcactgtgc cgttacaggc ccacgccagt aagggtaatg ccattgtggt gagcgccagt
    32221 gcggcaaaac cgtaatggct atgaatcaac aggccaagcg gagcaccaac agcgagggca
    32281 ccgtaaatag ccattccgtt ccatgacatc actttgccgg aatgttttgg cccaacgatg
    32341 cctaatcccc aggtcagagc gcccgtcagt aactggcttt caccaaagcc aagaatcaaa
    32401 cgcccgacga ccaacagagc aaatttgaac ggtgcgggga cgggcaaaat cgccgccagc
    32461 agcaacgcgc cccccgccag accacaagct aacatcccct gaagcgccga acgttttgcg
    32521 ccatattgat cggccagtcg cccggcgtaa ccacgcgtca gcaccgtagc cagaaactga
    32581 atcccgacgg caatgccgac catagtgttg ccatagccca gttcatgatg aacaaacagc
    32641 gggataaccg gcaacggtaa acctacggtc atgtaggtga gaaaaaccgc acaagcgatg
    32701 cgaaagagcg aaaaattggc agaagatcgt gtttcggttt ggcttacagc agtcatgcat
    32761 tactccagaa tgcagcgcaa ggcgaggagt atccccgtct catctctctg atttcagggt
    32821 tacggtgcgt tggcaggatt taacgcgtac gtcttttctg aaggaaatcg acaaagcggg
    32881 aagtttgcct ggaactggcg gcgattgtca atgatgtgaa aaagggaacc atcgggttcc
    32941 cttttgcgtt agtgccggag gccgcattgg cgcgcaacgt cggatgcggc gctggcgcgt
    33001 cttatccgac ctactccgaa ttaatctttc agcttgcgca ttaccagcgt ggcgttggtg
    33061 ccgccgaagc cgaagctgtt agacataact gtggtcagtt cgcgatcggt cgtttcggtc
    33121 acgatgttca gacccgcagc ctgctcgtcc agctcttcaa tgttgatgct cggggcgata
    33181 aaaccgtgtt ccagcatcag cagagagtag atagcttcct gtacgccagc agcgcccaga
    33241 gagtgaccgg tcattgcttt ggttgcagaa atcgccgggc tcttatcgcc gaacacttca
    33301 cggatagccg ccagctcttt cacgtcgcca accggagtcg aagtaccgtg ggagttcagg
    33361 tagtcgattg gagtatcaac accgtgcatc gccatctgca tacaacgtac tgcgccttcg
    33421 ccagacggag caaccatgtc tgcgccatca gacgttgcgc cgtagccaac gatttcagcg
    33481 tagatatgag caccacgcgc cagcgcgtgt tccagctctt caaccactac cataccgcca
    33541 ccgccagcga taacgaaacc gtcacggtga gcgtcgtaag tacgggaggc tttttccggg
    33601 gtgtcgttgt atttagtaga cagcgcaccc atcgcgtcga attcgcaagc catttcccag
    33661 cacagctctt cgccgccgcc agcaaacacg atgtcctgtt tgcccagttg gatctgctct
    33721 actgcgttac cgatacagtg tgcggaggtc gcacacgcgg agctgatgga gtagtttaca
    33781 ccgtgaattt taaacggcgt ggcgaggcag gcagaaacgc cggatgccat cgctttggtg
    33841 accacatacg ggccaaccgc tttcaggccg cgcgggccgc gcattgcgtc agcgccgaaa
    33901 acctggaaac gcggggaacc gccgccggaa cctgcaatca ggccaacgcg cgggttattc
    33961 tggtaagctt ccggggagag accagcatct gcgattgcct gctccataga aaggaatgca
    34021 taaatggatg cgtcgctcat aaagcgcaca actttgcggt caatgaggcc agtggtatcc
    34081 agttttacgt tgccccagac gtggctacgc atgccggaat ccttcagctc ctgagagaaa
    34141 gtgatccctg aacgtccttc acgcagagat gccaggactt cctgctggtt attaccgatg
    34201 ctggaaacga tgcccaggcc agtaatcact gcacgtttca ttcaatacct ctgtaagtcg
    34261 cactatagta agtttcgaat ggcacaatag cgtacacttg tacgccgaac aagtccgatc
    34321 agacgattaa tagagaaatt tgcgcagcct gacacacatc gttaagatcg ttagactgcc
    34381 cgtcagacga gtaacttacg tgaaacacta ctccatacaa cctgccaacc ttgaatttaa
    34441 tgctgagggt acacctgttt cccgagattt tgacgacgtt tacttttcca atgacaacgg
    34501 actggaagag acgcgttatg tttttctggg aggtaaccaa ttagaggtac gctttcctga
    34561 acatccacat cctctgtttg tggtagcaga gagcggcttc ggcaccggat taaacttcct
    34621 gacgctatgg caggcatttg atcagtttcg cgaagcacat ccgcaagcgc aattacaacg
    34681 tgtacatttc attagttttg agaaatttcc cctcacccgt gcggatttag ccttagccca
    34741 tcaacactgg ccggaactgg ctcagtgggc agaacaactt caggcgcaat ggcctatgcc
    34801 cttgcccggt tgccatcgtt tattgctcga tgaaggccgc gtgacgctgg atttatggtt
    34861 tggcgatatt aacgaactaa cctgccaact ggacgattcg ctaaatcaaa aagtagatgc
    34921 ctggtttctg gacggctttg cgccagcgaa aaacccggat atgtggacgc aaaatctgtt
    34981 taacgccatg gcaagactgg cgcgtccagg cggcacgctg gcgacattta cgtctgccgg
    35041 ttttgtccgc cgcggtttgc aggaagccgg attcacgatg caaaaacgta aaggctttgg
    35101 gcgcaagcgg gaaatgcttt gtggagtgat ggaacagaca ttaccactcc cctgctccac
    35161 gccgtggttt aatcgtacgg gcagcagcaa acgggaagta gcgattatcg gtggtggtat
    35221 tgtcagcgcg ttattgtcgc tggcgctatt acggcgcggc tggcaggtaa cactttattg
    35281 cgcggatgaa gcccccgcgc tgggtgcttc cggcaatcgc cagggggcgc tgtatccgct
    35341 tttaagtaaa cacgatgaag cgctaaaccg ttttttctct aatgcgttta cttttgctcg
    35401 tcggttttac gatttattgc ccgttaaatt tgatcatgac tggtgcggcg tcacgcagtt
    35461 aggctgggat gagaaaagcc agcataaaat cgcacagatg ttgtcgatgg atttacccgc
    35521 agaactgact gtagccgttg aggcaaatgc ggttgaacaa attacgggcg ttgcgacaaa
    35581 ttgcagcggc attacttatc cgcaaggtgg ctggctgtgt ccggcagaac tgacccgtaa
    35641 tgtgctgaaa ctggcgcaac agcagggttt gcagattcat tatcaatatc agttacagga
    35701 tttatcccgt aaggatgact gttggttgtt gaattttgca ggagatcagc aagcaacaca
    35761 cagtgtagtg gtactggcga acgggcatca aatcagccgt ttcagccaaa cgtcatctct
    35821 gccagtatat tcggtggccg gtcaagtcag ccatattccg acaacgccgg aactggcgga
    35881 gctgaagcag gtgctgtgct atgacggtta tctcacgcca caaaatccgg cgaatcagca
    35941 ccattgtatt ggtgccagtt atcatcgcgg cagcgaagag acggcgtaca gtgatgaaga
    36001 tcagcagcag aatcgccagc ggttgattga ttgtttccca cacgcgcaat gggcaaaaga
    36061 ggttgatgtc agtgataaag aggcgcgctg cggtgtgcgt tgtgccactc gcgatcatct
    36121 gccaatggta ggcaacgtgc ctgattatga cgctacactc gtggaatatg cgtcgctggc
    36181 ggagaagaaa gatgaagcgg taagcgcgcc ggtttatgac gatctcttta tgtttgctgc
    36241 tttaggttcg cgcggtttgt gttctgcccc gctgtgtgcc gagattctgg cagcacagat
    36301 gagtgaagaa ccgattccga tggatgccag tacgctggcg gcgttaaatc cgaataggtt
    36361 atgggtgcgg aagttgttga aaggtaaagc ggttaaggcg gggtaatctg ctctggcatt
    36421 gtttgtcgga tgcggcgtaa acgccttatc cgacctacgc gttacctgta ggcctgataa
    36481 gacgcgataa gcgtcgcatc aggcatggtg ctccagacgt cacaacttac tgctgtgagg
    36541 cctgctgaaa caggtgttcc cacatatcgg taaccagcgc ctggtcacgc ggtgacaatt
    36601 cacccgcgcc aatggctttt tccagactct ggctaacggt tgtatgtacc gcctgagcgg
    36661 agtggtcgtc accactttcc agttctgcga tggctaatgt caggtggcca cgcaaatacc
    36721 cactggcaaa caactcatca tcactggcat ggtcaaccat accgtcgatt aatgccagaa
    36781 tgcgtgattc aaactccgcg atcatcttct ttcctcattg taaaacgtgg cgctggcttc
    36841 agttgagcgc ttccggccac gggaactgtt ccgccgtaag ttccggcgtg tgataataat
    36901 tttgtagtgc tttaatgaag cgtgccggac gttcgggaat gccgtttgcc agataatcca
    36961 tcacctgcgc atgtacccgc cgttgaaaca ccacgcggtc cggttcgaaa tccccttcca
    37021 gattgtcgca gctaacatta aacggatatc ccgccgccac gcagaacaac cagtccagcg
    37081 cctgcggctt cacttcaaca tcttcaaact ggctttgcgt ttgggcatcg cgtccgtcgg
    37141 gacaatacca gtagccgaaa tcaaccagct cacggcgcgc tttcccggca atacaccagt
    37201 gcgaaatctc atgaattgcg ctggcataaa agccatgagc aaagacgatt cggttatatg
    37261 gtacttccgc atcagcagga agatagatcg gttcgtcgtc gcctttaatc agacgggtat
    37321 taaaatcatc ggaaaagcag ctattaaaaa tttcaatcaa ctgctcgtag tggtgtgtac
    37381 tgttcattag ttcatcccca accagtggag gatctcctgt ccgtggctat cataaagtaa
    37441 tttggcactc atcaccgccg agacgataac gatcattggg cggatcagct tttgtccttt
    37501 gctcaacacc agtcttgacc ccatgcgcgc gccgaggaac tgtcctacca acatcacaaa
    37561 tcctgtcgcc caaatcactt tgccaccgag aataaacagc agcaaaccgc caatgtttga
    37621 cgttgcgtta agtaatttgg cgtgagcagt ggctttggcg aggttgaatc cgcacagcgt
    37681 aacgaaggcc agcgcgtaaa acgatccggc agccgggcca aaaaatccat cgtaaaaacc
    37741 gacgcaacca cctgcaatta acgcgaaggg taaaccgtac atccggcgct ggcgatcttc
    37801 ttcacccagc tttggcatca gtaagaaata aagaccgata caaattacga ggatcggtaa
    37861 aatctgccgt aagacatcgg cctgaacata ctgcaccagt agcgcgccgc tcattgagcc
    37921 gacaaaggtc atggcgatat tgagtttttg atcgcttaaa ctaaccactt tacggcgaat
    37981 aaagtagata gtggcggaaa tagagccacc gcaggcttgc agtttattgg ttgccagcgc
    38041 gttagcggga gacatccccg ccgccatcaa tgccggaatg gtgagtaacc caccgccacc
    38101 ggcaatcgag tcgataaatc ccgccagcat ggcgacaaaa aagagaactc ccagcagcag
    38161 cggggaaacc ataaacaggc tattaaacgt ttccattaga tcacgtgctc atccagtagc
    38221 gcctggcagg aaggcggcaa cggaggcggt gtcttcttct caggctttgt tgttcccggt
    38281 tttggaggtt caaaccagct ttgcagttct gccccgcaac catcgcctgg tggcggtaaa
    38341 ggttgatcct cacactccag actatcggca ggacaacgta atcgtacatg catatgcgcg
    38401 cgatgctgga accagggtcg cactttgcgc aaccagtcgc gatcggtgcc cgcatcaagg
    38461 caaagttgtt gtttaatcgc cggattaaca aaaatgcgcg tgacgtcttt gtcctgggcg
    38521 gcgagtttga tcaagctgaa aatttctggc ttccacagcg cggggacaac gtgtttaccg
    38581 tcgcgggaaa ctaagtccag agcttgcggg cgcaagagct gcgcggaggt ccagcgtgtt
    38641 ttcggcagtt gcaggaagat atcgacatcc agtccggtct ggtggctggc gtgaccgccg
    38701 ttgaaacgac caccagcagg catccccata tcgccaatca gcaccgtacc catgcccaga
    38761 ttgctcacct ggcgactcag acgctggata aacatcacca gatccggatg tccgaaatag
    38821 cgacgctgat cggtacgcat gacctgataa tgttcggact gtatcggcaa cgtgtcagca
    38881 ccgacgatac agccattaga aaaactgcct atcgattgtg cgctacccgg cacaggttgg
    38941 gttatttttt gccacggcgt cgctgccagg ctggcgctac tggcaagcag ggccagcagc
    39001 gcaatcgcgg ttttgttcat tttttaccag cgtggaatat cagtcttcac atcagcattt
    39061 tgcgcccgtt gccgtaacag gtgatccatt aaaacgatcg ccagcatcgc ttccgcgatc
    39121 ggcaccgcgc ggatcccaac acaaggatcg tgacgacctt tggtgatcat ctcaacttct
    39181 tcgccaaagc ggttaatggt gcgccccggc acggtaatgc tggaggttgg cttcagcgcc
    39241 atatgggcaa tgatttgctg cccgctgctg ataccgccaa gaatgccgcc cgcatggttg
    39301 ctctggaaac cgtctttggt gatttcgtcg cggttctggc tgccacgcag cgctaccacg
    39361 tcaaaaccat caccaatttc cacacctttc accgcgttaa tgctcattag cgcatgggcg
    39421 atgtcggcat ccaggcgatc aaagaccggc tcgccaagtc cggcgggtac gccactggca
    39481 acgacggtga ctttcgcgcc gatggagtcg ccctcttttt tcagcgcgcg catcagttca
    39541 tctaaggcgt cgattttgtc cgggtccggg cagaaaaacg gattttgctc gacctgagac
    39601 cagtctttga tttccagtgg aatgtcgccc atctgggtca ggcaaccgcg aatctcaatg
    39661 ccaaatttct cggcgagata ttttttggca atcgcccctg ccgccacgcg catagcggtt
    39721 tcacgggcgg aagaacgtcc accgccgcga taatcgcgca ggccgtattt ttgttcatag
    39781 gtgtaatcgg catgacctgg acggaaaacg tccttaatcg cactataatc ctgagaacgc
    39841 tggtcggtat tttcgatcaa caagccaatg ctggtgccag tagtaacacc ttcaaaaaca
    39901 ccggagagaa ttttgacctg atccggctcg cggcgctggg tggtatagcg cgatgtccca
    39961 gggcgacgac gatcaaggtc atgttgcagg tccgcttccg tcagcggaat gcctggcgga
    40021 acaccatcga cgatgcagcc gagcgccagc ccgtgcgatt cgccgaaggt ggttacgcga
    40081 aagagttgtc caattgtgtt tccagccatc acggctccgt tattgttgtg tttgcgtgtt
    40141 tacttaatct ttataaatcg cgaaatgttc acgggccgcc agcagctgct ctttggtgag
    40201 cataaacaca ccatcgccgc cgttatcaaa ctccagccat gtgaacggaa catccggata
    40261 ttgttccata agatgtacca tgctgttgcc cacttcacaa atcagcacac catcatcagc
    40321 gaggtaatct gccgcgttac cgagaatgcg acgcgtcagt ttcaggccgt cggtgccaga
    40381 tgccagaccg agttccggct cgtggcggta ttcgtttggc aggtcggaca tatcttccgc
    40441 atcgacgtac ggcgggttgg tgacgatcag gtcgtactgc actttcggca agtcacggaa
    40501 caagtcggaa cgaatcggaa tgacgttgtg gatcaaaccg tgttcttcga tgttctgttc
    40561 agcaaccgcc agcgcgtctg gagagatgtc caccgcgtcg acttccgctt ccgggaaggc
    40621 ataagcacag gcaatggcga tgcagccgct accggtacac atatctaaaa tgtgttgcgg
    40681 ttgcttgctg atgagtccgg caaatttatt gttgatcagt tcaccaatcg gcgagcgcgg
    40741 caccagcaca cgttcatcga cgtaaaattc atgaccgcag aaccaggctt tgttggtcag
    40801 gtaggccacc ggaatgcgtt cgttgacgcg gcggatcacg cgctcaacaa tacggtgttt
    40861 ttcgctggaa gtcagacgcg cggtgcgcat atcttccgga atatccagcg gcaggtagag
    40921 cgaaggcaac accagttgca cggcttcatc ccaaggatta tcggtgccgt gaccatacca
    40981 gatatttgcc gcgctgaagc ggctcaccga ccagcgcaac atgtcctgaa tggtttgcag
    41041 ctcatttact gcttcatcaa cgaaaatttt atccacgtat tcctccaggg catgatcgca
    41101 ataatttcgg cggctagttt gccatgaaga tgacgataaa tcagcattca cgcgcggtga
    41161 gtgaggaaaa atacgtttaa aacgatcgat tgtgctacga gtcgggtaaa ctgtaggaaa
    41221 attagaaata gagaaagata aatgaaaaag aaaacaacac ttagcgagga ggaccaggcg
    41281 ctgtttcgcc agttgatggc gggtactcgc aagattaagc aggacacgat tgtccaccga
    41341 ccgcagcgta aaaaaatcag cgaagtacca gtaaagcggt tgattcagga acaggccgat
    41401 gccagccact atttttcgga cgaatttcag ccattattga ataccgaagg tccggtcaaa
    41461 tatgttcgcc cggatgtaag ccattttgag gcgaagaagt tgcgacgcgg tgattattca
    41521 ccggagttgt ttcttgattt gcacggtctg acgcagctac aagccaaaca ggaactgggg
    41581 gcgttgattg ccgcctgccg ccgtgaacat gtgttttgcg cctgcgtgat gcatgggcac
    41641 gggaagcata ttttgaagca gcaaacgccg ctgtggctgg cgcaacatcc gcatgtgatg
    41701 gcatttcacc aggcaccaaa agagtatggt ggcgatgcgg cgttgttggt gctgattgaa
    41761 gtggaagagt ggttgccgcc ggaattaccc tgaatattaa aaggtggatg agtcgggcca
    41821 tttagccttg gatactggcg tctcaaccct ggcgataatg aacgtaataa tcgggcatta
    41881 attgaaaaac gtaagcagct ttccgacagc cttctgtggg ctaatggcat tgccccttca
    41941 gaggggcttg aggatgacga cctgctgcaa gcgcttatcg acgaattact caccctcgtc
    42001 gacggttatc tggcaccttt cgctcctgtg ttggcgaata ttctaaaaaa ctggatgacc
    42061 gcatgacata acggacaacg gggccagatg gccccgcatc gtgtgtggtg tatgcgctac
    42121 agttcgttca acgtgtactg gaagcggcag gtggaattga tcggccgtct gctgctggta
    42181 tcaacatgca ctaccatgtc gtgtagctgt ttgtccgtaa tggggtgccg gttgggcgcc
    42241 accgtaacat tagcattatc acagcctgat gatgaaaacg tgatcatttg cgcaacccag
    42301 cgacagtaag cgccctcttc attcatttgc gagccatcac atagtttgcc gtctttcatc
    42361 agataggttg aaagcgtttt ttcaattact ccgcccgtcg attgcaaaga cagtgttttc
    42421 gttttattga tatagttttc atcccatgaa acatcatacg gcgtaactga cacggttggg
    42481 cacacggaca cggttgcatt tggtccataa tagatatctt tatcaacaga accgaccaca
    42541 acctggctgg tggctctgat actcactaac cacgggcagc ctcccacatc accatcgacg
    42601 gtaatatcca gaggcagctt gagacattcc cagcggctgt ctttggcttc cagtgcctcc
    42661 atgcccggac aaaatgaatt atttacaatt gtcccatctt ttaccgtaat cgtcggcgca
    42721 ggagaactgc ccgttcctgt ccatgtgcga ttgggtaagt tgccaagcgt cataacataa
    42781 ttactttgat aataggtacc gtagtaaaca ctcgaaacct cagcaacatc aagatacagt
    42841 tcgacctgac tattccagcc aaacgtagca tagtagatat acacattacg aaatgtctgc
    42901 cccatcccgg cccgactacc gagcataaaa accatcagga tcaaaagacg aaacagcaag
    42961 ttagtttttt tcatcttata tgtactcaaa cgttgcggtt aaggaggcgg taaactcacc
    43021 catcgtcact tcccggccgc ttttcgcctg gagccaggca ttaaaattca gagcgttatc
    43081 accgttactc agcacaaagg ttgcgccagt ggtgctgttt attggcaccg ccgttccgtc
    43141 ggttttttcc atgccaatcc cgacgccctg tgcacctgat gaagcatcca gcgccagaaa
    43201 accgggctgc tcgctgtctt ccgtgccggt cagtgtcacc cggacttcat actcggctgg
    43261 ccctttacaa tctttcagtt gaaacaccac tggcacccgc gccgactgcc cggtcaccat
    43321 caggtctttg cggctgacgg tggggaaatg cacctccacc agcgactcgc cctgaatcac
    43381 cagcgtgcag gacttgctga gtaaattgcc gtagaaatgc aggttgtcag ccgcctgcgc
    43441 ttggggcatc agcagcaccg ccgccagtgc cacgtagcgg taatgtgtgc gagtcagatt
    43501 cattggtaat ccaccgttaa ggttgcccag gcagagaagt tcccttcact gagcgccgcg
    43561 tcgctggctt ttaccggcac cgcgatcagc gtcggcgggc tcgccgggtc tattgtcaac
    43621 tgttcgccca ctttaaacgg ctggccgttt tgctgaagct caatccccag gcctgcgacg
    43681 tcggtttcaa tggcggcgtc atcgtagctg gtctggtggc ccgtccaggt caggctcatc
    43741 tcccaggcgt cgtcgcgcac atcaggatcg caggtgatgg tgtaaggcac gtcctggcgg
    43801 aaattgtcac cgttgatgtt gtcgatgatc acattgctga acgccacttc aatgctctta
    43861 ccgtcgctga tggtacaggc gggcggcgag accagcgtgc cgtgaaaagt aacgtcgtta
    43921 accgcggcct gcgacaggcc agcaaaaccg taaagtaagc accacgcgag tcgtttcatt
    43981 ggtagtccac caccatggtc atggtcgcgt taaactccga tgccatcagt tgcacaccgc
    44041 tctgcttcac cgggacggct tcgaattctg gccgggtgtt gatgttgaac ggcagccagc
    44101 tagcgttgcc cacggccacc agcgaatggt cggtggcatt ctgaatgcga atgccgaagc
    44161 catcgattcc ggtactcagt accgtttcac cattaatcgt ggtggtggtg gcctgaagct
    44221 gcattcgcaa gtcgtcgctg acgctgtttg agcaattcat ggtgtagccc accggctgac
    44281 ggtagttggt gccatcaatg cctttaatga ccacactgcc gaactccacc gcgcttccct
    44341 gtatcgagca gggcggtggc ccatccacca gcacgcgcag cgtcaggttg acgattttgc
    44401 cgcccgccat tgccggtgaa acggtcacgc cacaggccag caatagcgcg gcagatccgg
    44461 ccagaaagaa cgctttcatg ttgcttctcc cgttatcagc ccttgctgct ttgatcttca
    44521 tccaccttgc aggtgttccc ttcacaacgg aagatcagcg gcatgcgcgc gccgtagtcg
    44581 ttgatgtagg tcagcaccgg ggcgctcgcc aattttgcct ttaacggttg tgacgttttc
    44641 ggtggcatca ccagcggttt aaacccggcg gcagtcagcc ctttcttctg tgtgctggca
    44701 tcgctgatga tgacgtggta cggcgtcggg ttattgacgg tatagtcctg gccgctgcgc
    44761 gtgagtgtga ccttaaactg ccacggtttt ctcatgtcaa tcttctccag cgcctttggt
    44821 cgccagaaca gcttgatgcg cgtttgcagc gcaatttgca gggtgttggc tttcccggac
    44881 tttggcggga tctcacgcac gttgtagtag aacaggcttt ctcggtcgga cggcagcgtg
    44941 tgaatgtccg gcagcgcctg aatcttcacc tggccgttca tcatagcgtc aatacgctgc
    45001 accggcggca gcaccgccag cggcgaagtg attttgttgc ccttctcgtc ttccagccag
    45061 ctttgcgcca ggtaaggcag cttttcgttg ttgttacgca acgtcacgct gatcgattta
    45121 tcgctttcgt taaacaccag acgggtgcga tccggggtga ccgatgcatg cgccgtcagg
    45181 ctggcggtgg taagcagtgc catcagcgct gtgctgacga gcattggaca ggataattta
    45241 taagtcattg atcgttcctt aatcagatta ttgagttgct gaaattgacg tcggcgcttc
    45301 cgttggtgtg acttgccgcg tctggtcctg aataacaggc ttaatttctg gcgctgaaga
    45361 atcaggggct gccgttccct tttgttcaca cggcaacaac aggccgctga acaaatcggc
    45421 aggtagcggc ttcggcaata cgatctcgca ttgtgcgctg ccttcccaga acgccatcat
    45481 atgctcgtcc gcattgaccc ccgccagata gacattgcct tcatcatcca caatgccaac
    45541 ctgctgctgc tcgtcgtttt tcacttccgc gccaaacggc gggtagctac cgtcacgcaa
    45601 ccgcagtacc gccatcgctt tttgcccgct gatcactttg aatttacgat agccgattgc
    45661 gccttccgtc agagtcgcct gcaccaccga ctgggtggct tccgcatctt ccggcaagtt
    45721 gtttaaatcg atatacgcct gattgcgata gtagttgtta atatcggcga ccaccgcctt
    45781 gccaaacata ttggtgtaca ccggcgcgcc gttgctttcg accggcacgt tggcaatccc
    45841 gtcggcgtca atcagcaggc gcgtgccgcc catgttctgg gtacggtgca gcgccccacc
    45901 atgcgcggtc agcgttgccc cgccctgtaa ggcgatcccc gccgaacggt attccccttc
    45961 atggtagttg gcactgagat caaccttcgc cagcgaaccg tcgtggctca gataaccatc
    46021 cacgctgccg tgctgctcgc tggtgccaac gttaacctgg tagtgcgttg cgtcatcgac
    46081 gcgcttaaag taaccgacct ggctgctgtc cgatccgctg ccgtaggaac cgttgtaggt
    46141 cacggtgctg ctgtcgctcc acggaatgct catgctgagg tacatcccct tatccgcgtt
    46201 atcgtcatat tcgtagcgat agccggtcac cgagatgctc atgttgcgaa tactccccat
    46261 attaaaatag tgggaaaaca tcaggttata gtttgtctgt tccgggcgat cccagtaagt
    46321 acgatgggag tagttgagat agatcgagac acccgcagcg gcaaagttct ggttataggt
    46381 gatcgtatac atctctttgt cgttaccggt ccgcgtcatg tccgactggt tcgcgtccag
    46441 atactcgctc atggtcatga agttctttgt tggtcttatc tataaaaaaa ctcaatttag
    46501 taatacttaa acactttatg tagattttat taattaatac atctacaaaa tactttcacc
    46561 actttccaac aaa
//
LOCUS       DAZFJL010000019        45637 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFJL010000019 DAZFJL010000000
VERSION     DAZFJL010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.019"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(332..874)
                     /gene="ycfP"
                     /locus_tag="ACHJ8C_001078"
     CDS             complement(332..874)
                     /gene="ycfP"
                     /locus_tag="ACHJ8C_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO5550972.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(897..1922)
                     /gene="nagZ"
                     /locus_tag="ACHJ8C_001079"
     CDS             complement(897..1922)
                     /gene="nagZ"
                     /locus_tag="ACHJ8C_001079"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO5550973.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVMADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(1933..2757)
                     /gene="thiK"
                     /locus_tag="ACHJ8C_001080"
     CDS             complement(1933..2757)
                     /gene="thiK"
                     /locus_tag="ACHJ8C_001080"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO5550974.1"
                     /translation="MPFRSNNPLTRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRL
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2738..3379)
                     /gene="lpoB"
                     /locus_tag="ACHJ8C_001081"
     CDS             complement(2738..3379)
                     /gene="lpoB"
                     /locus_tag="ACHJ8C_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO5550975.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PIPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3393..3770)
                     /locus_tag="ACHJ8C_001082"
     CDS             complement(3393..3770)
                     /locus_tag="ACHJ8C_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO5550976.1"
                     /translation="MRKGCFGLMSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3773..4132)
                     /gene="hinT"
                     /locus_tag="ACHJ8C_001083"
     CDS             complement(3773..4132)
                     /gene="hinT"
                     /locus_tag="ACHJ8C_001083"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO5550977.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGCPLGPMLAHKGL"
     gene            4466..6655
                     /gene="fhuE"
                     /locus_tag="ACHJ8C_001084"
     CDS             4466..6655
                     /gene="fhuE"
                     /locus_tag="ACHJ8C_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO5550978.1"
                     /translation="MLSTQFNRDNQHQAIIKPSLLAGCIALALLPSAAFAAPVTEETV
                     IVEGSATAPDDGENDYNVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTMLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLTPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(6715..8148)
                     /gene="ptsG"
                     /locus_tag="ACHJ8C_001085"
     CDS             complement(6715..8148)
                     /gene="ptsG"
                     /locus_tag="ACHJ8C_001085"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO5550979.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8443..9240)
                     /locus_tag="ACHJ8C_001086"
     CDS             complement(8443..9240)
                     /locus_tag="ACHJ8C_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5550980.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9251..10255)
                     /gene="holB"
                     /locus_tag="ACHJ8C_001087"
     CDS             complement(9251..10255)
                     /gene="holB"
                     /locus_tag="ACHJ8C_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO5550981.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVQSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHYGAAHVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10252..10893)
                     /gene="tmk"
                     /locus_tag="ACHJ8C_001088"
     CDS             complement(10252..10893)
                     /gene="tmk"
                     /locus_tag="ACHJ8C_001088"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO5550982.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTNWVKELDA"
     gene            complement(10883..11905)
                     /gene="yceG"
                     /locus_tag="ACHJ8C_001089"
     CDS             complement(10883..11905)
                     /gene="yceG"
                     /locus_tag="ACHJ8C_001089"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO5550983.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11908..12717)
                     /gene="pabC"
                     /locus_tag="ACHJ8C_001090"
     CDS             complement(11908..12717)
                     /gene="pabC"
                     /locus_tag="ACHJ8C_001090"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO5550984.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSITAYPAHYDRLRNEGMMLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12837..14078)
                     /gene="fabF"
                     /locus_tag="ACHJ8C_001091"
     CDS             complement(12837..14078)
                     /gene="fabF"
                     /locus_tag="ACHJ8C_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO5550985.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14166..14402)
                     /gene="acpP"
                     /locus_tag="ACHJ8C_001092"
     CDS             complement(14166..14402)
                     /gene="acpP"
                     /locus_tag="ACHJ8C_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO5550986.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14613..15347)
                     /gene="fabG"
                     /locus_tag="ACHJ8C_001093"
     CDS             complement(14613..15347)
                     /gene="fabG"
                     /locus_tag="ACHJ8C_001093"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO5550987.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ITDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15360..16289)
                     /gene="fabD"
                     /locus_tag="ACHJ8C_001094"
     CDS             complement(15360..16289)
                     /gene="fabD"
                     /locus_tag="ACHJ8C_001094"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO5550988.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIGKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYIAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16305..17258)
                     /gene="fabH"
                     /locus_tag="ACHJ8C_001095"
     CDS             complement(16305..17258)
                     /gene="fabH"
                     /locus_tag="ACHJ8C_001095"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO5550989.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17326..18396)
                     /gene="plsX"
                     /locus_tag="ACHJ8C_001096"
     CDS             complement(17326..18396)
                     /gene="plsX"
                     /locus_tag="ACHJ8C_001096"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO5550990.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18477..18650)
                     /gene="rpmF"
                     /locus_tag="ACHJ8C_001097"
     CDS             complement(18477..18650)
                     /gene="rpmF"
                     /locus_tag="ACHJ8C_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO5550991.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18702..19223)
                     /gene="yceD"
                     /locus_tag="ACHJ8C_001098"
     CDS             complement(18702..19223)
                     /gene="yceD"
                     /locus_tag="ACHJ8C_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO5550992.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19421..20005
                     /gene="yceF"
                     /locus_tag="ACHJ8C_001099"
     CDS             19421..20005
                     /gene="yceF"
                     /locus_tag="ACHJ8C_001099"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO5550993.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20117..21076)
                     /gene="rluC"
                     /locus_tag="ACHJ8C_001100"
     CDS             complement(20117..21076)
                     /gene="rluC"
                     /locus_tag="ACHJ8C_001100"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO5550994.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            21649..24834
                     /gene="rne"
                     /locus_tag="ACHJ8C_001101"
     CDS             21649..24834
                     /gene="rne"
                     /locus_tag="ACHJ8C_001101"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO5550995.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETSTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKSAPATPAAPA
                     QPGLLSRFFGALKALFSGGEETKPSEQPTPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     PRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAAAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEVAEVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            24968..25261
                     /locus_tag="ACHJ8C_001102"
     CDS             24968..25261
                     /locus_tag="ACHJ8C_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO5550996.1"
                     /translation="MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEA
                     ARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPNAALRKTMQTPAPWEQKK"
     gene            25258..25752
                     /locus_tag="ACHJ8C_001103"
     CDS             25258..25752
                     /locus_tag="ACHJ8C_001103"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5550997.1"
                     /translation="MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTF
                     VSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEPIPVVVLGRLAIDKSLQGQGIGR
                     AMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
                     ECLK"
     gene            complement(25847..26800)
                     /gene="flgL"
                     /locus_tag="ACHJ8C_001104"
     CDS             complement(25847..26800)
                     /gene="flgL"
                     /locus_tag="ACHJ8C_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO5550998.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEADGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(26812..28455)
                     /gene="flgK"
                     /locus_tag="ACHJ8C_001105"
     CDS             complement(26812..28455)
                     /gene="flgK"
                     /locus_tag="ACHJ8C_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO5550999.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTNLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYIDGTAGNIEIPEKLLN
                     TGSLGGILTFRSLDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGKDFFAIGKPA
                     VLQNTKNNGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(28521..29462)
                     /gene="flgJ"
                     /locus_tag="ACHJ8C_001106"
     CDS             complement(28521..29462)
                     /gene="flgJ"
                     /locus_tag="ACHJ8C_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO5551000.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEEPTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGNSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(29462..30559)
                     /gene="flgI"
                     /locus_tag="ACHJ8C_001107"
     CDS             complement(29462..30559)
                     /gene="flgI"
                     /locus_tag="ACHJ8C_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO5551001.1"
                     /translation="MIKFLSALILLLVITAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(30571..31269)
                     /gene="flgH"
                     /locus_tag="ACHJ8C_001108"
     CDS             complement(30571..31269)
                     /gene="flgH"
                     /locus_tag="ACHJ8C_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO5551002.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(31322..32104)
                     /gene="flgG"
                     /locus_tag="ACHJ8C_001109"
     CDS             complement(31322..32104)
                     /gene="flgG"
                     /locus_tag="ACHJ8C_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO5551003.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(32276..33031)
                     /gene="flgF"
                     /locus_tag="ACHJ8C_001110"
     CDS             complement(32276..33031)
                     /gene="flgF"
                     /locus_tag="ACHJ8C_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO5551004.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVVGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(33051..34256)
                     /gene="flgE"
                     /locus_tag="ACHJ8C_001111"
     CDS             complement(33051..34256)
                     /gene="flgE"
                     /locus_tag="ACHJ8C_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO5551005.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNTFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLVFNANGVLTSNPTANISTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(34281..34976)
                     /gene="flgD"
                     /locus_tag="ACHJ8C_001112"
     CDS             complement(34281..34976)
                     /gene="flgD"
                     /locus_tag="ACHJ8C_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO5551006.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34988..35392)
                     /gene="flgC"
                     /locus_tag="ACHJ8C_001113"
     CDS             complement(34988..35392)
                     /gene="flgC"
                     /locus_tag="ACHJ8C_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO5551007.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(35396..35812)
                     /gene="flgB"
                     /locus_tag="ACHJ8C_001114"
     CDS             complement(35396..35812)
                     /gene="flgB"
                     /locus_tag="ACHJ8C_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO5551008.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTKHIPAQALTPPSAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35967..36626
                     /gene="flgA"
                     /locus_tag="ACHJ8C_001115"
     CDS             35967..36626
                     /gene="flgA"
                     /locus_tag="ACHJ8C_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO5551009.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCDQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMISGQVVSGVVDADGNILIN
                     L"
     gene            36702..36995
                     /gene="flgM"
                     /locus_tag="ACHJ8C_001116"
     CDS             36702..36995
                     /gene="flgM"
                     /locus_tag="ACHJ8C_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO5551010.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDFQSN"
     gene            37000..37416
                     /gene="flgN"
                     /locus_tag="ACHJ8C_001117"
     CDS             37000..37416
                     /gene="flgN"
                     /locus_tag="ACHJ8C_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO5551011.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLYMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNSANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(37456..38991)
                     /gene="murJ"
                     /locus_tag="ACHJ8C_001118"
     CDS             complement(37456..38991)
                     /gene="murJ"
                     /locus_tag="ACHJ8C_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO5551012.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(39101..40024)
                     /locus_tag="ACHJ8C_001119"
     CDS             complement(39101..40024)
                     /locus_tag="ACHJ8C_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO5551013.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATASSLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGNATLESGTLLTNESGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(40026..40673)
                     /locus_tag="ACHJ8C_001120"
     CDS             complement(40026..40673)
                     /locus_tag="ACHJ8C_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO5551014.1"
                     /translation="MKYQLTAMEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLASREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVENPPAVTDMLNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(40684..41268)
                     /gene="rimJ"
                     /locus_tag="ACHJ8C_001121"
     CDS             complement(40684..41268)
                     /gene="rimJ"
                     /locus_tag="ACHJ8C_001121"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO5551015.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMISEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALSAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            41504..42712
                     /gene="mdtH"
                     /locus_tag="ACHJ8C_001122"
     CDS             41504..42712
                     /gene="mdtH"
                     /locus_tag="ACHJ8C_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO5551016.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            42776..43423
                     /gene="grxB"
                     /locus_tag="ACHJ8C_001123"
     CDS             42776..43423
                     /gene="grxB"
                     /locus_tag="ACHJ8C_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO5551017.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            43557..44117
                     /locus_tag="ACHJ8C_001124"
     CDS             43557..44117
                     /locus_tag="ACHJ8C_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5551018.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            44223..45269
                     /gene="pyrC"
                     /locus_tag="ACHJ8C_001125"
     CDS             44223..45269
                     /gene="pyrC"
                     /locus_tag="ACHJ8C_001125"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO5551019.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTNADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            45343..45588
                     /gene="dinI"
                     /locus_tag="ACHJ8C_001126"
     CDS             45343..45588
                     /gene="dinI"
                     /locus_tag="ACHJ8C_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO5551020.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11058 a        12320 c        11346 g        10913 t
ORIGIN      
        1 aggttacagc aatgaaaatg ttcttcgcta ttgagaataa catgaatggt gcattggtca
       61 taccaaaatt gatgttaatc aagttttgtt gctgaaaaga taggcgactt taccgggtga
      121 gatcaaaaaa cagagtttat tgtttattat ttgagcgaat ttgtgagagt gatatagagt
      181 gaggggtgta tggagtgcgt tcggtaggcc tgataagagg cgcatcaggc attgatgctc
      241 gactgtcgga tgcggcgtgg acgccttatc cgacttacag aattcggagc gcatcaggta
      301 ccggcaaggt tacctgatgc gacgagggca tttacccgag ggttttgaac gctttaatgc
      361 gctgtaaatg cggggagata ttcttgaatt tgtgcgtctg ctcttcgtcc cagacaatct
      421 cgtaataatg atgcaactct tcagatgtcc gctggctgtt aagcgcttca tcattacgcg
      481 acaaaatcac caggcaacga tcgcgattct tctcacggaa gttggtcaca cacttagtgg
      541 caatatcggc atactcttcc gggcgatcaa tcttcccttc catgttctca taagggaaca
      601 aattggggtt gaagatcacc tggcggatat cacagagaaa accaatccgc tcagcccaat
      661 aaccgcccag accaacacca caaatgagcg ggcgctcgtc gacgttcagt tgcaacattt
      721 tgtccacttc tttgagcaaa tgctgcatat catgtttcgg atgccgcgta ctgtagctta
      781 ttagccgtac atccgggtca ataaactgca attgtaagac tttctcgtgg ttacccggac
      841 tgttagagtc aaaaccgtgc aaatagataa tcatcgcttc ctcacataag ccagggttaa
      901 tgacctgcct tctcttcctg ccagcgttca tgtaactgat tcagacgggc gctgatcgct
      961 ttccagcgag ccgagtccat cagttcctgt cgcgaaaatg aacctttatg atacaaacgc
     1021 gtaacacgtt ctgccttgat cggtgacaga ttatctaaca cgctgacggc ccctttacga
     1081 ttattgcaga ccaggatcat atcgcaacct gcatccagtg atgcctgtcc acgctcggca
     1141 taactgccca taatcgctgc gccttccatc gataaatcgt cagagaaaat cacgccgtca
     1201 aaacccaatt cctgacgcaa aacggttttc agccagtagg gagaaccgct cgccggacgc
     1261 ggatcaacat cactgtagat cacatgcgca ggcataatgg cgtcgagttt attttcgcgg
     1321 attaaggaac tgaagaccga catatcttta gcgcgaatct ccgcttgcgg acgtgggtcg
     1381 cacggtgttt ctttgtgtga gtctgccatt actgcaccgt gacctgggaa gtgtttcccg
     1441 gtagttttca ttccggcttc atgcatacca tcaataaacc ggctggcaat tgccagggct
     1501 ttttgtggat cggcatgata agaacgctcg ccaatcgccg cgctgatatg cccgacgtcc
     1561 agcactggcg caaagctgat atcaatatcc atagcaatca tttcgctggc catcaaccaa
     1621 cctgcctctt gcgccagttt tccgccttct tccattccca acagcgcagc gaatgattgt
     1681 gccgctggca agcgggtaaa accttcacga aaacgctgca cgcgtccacc ttcctgatct
     1741 accgccacca ccagatgatt gcgcgatgct gcgcggatct ggcgcaccag ttcacgtaac
     1801 tgggcaggat catgatagtt tcgcgtaaag agaatcagcc ctcccaccag cggatgcgcc
     1861 agtatttcac gctcttccgc gtccagttcg taaccttcga catccaacat tactggaccc
     1921 acactgctct ccttattgtt ttattaatag ctgccgccag gtgtcatcgg ccagcctgat
     1981 aaattgttga tcgccggttt gtcgccagcg gtactcaaac caccctgctt tgagcatcag
     2041 cagccaggga aaccatcgcc tgacctgacg ccataattgc gccggataaa tcttcgcgcg
     2101 agtggcatag tcattgacca attgccgatg ctggtcagta ttttccaccc acaccgccgc
     2161 cagttccagc gcgatatcac catctccggc atactcccag tcgatgagtt ttaaccctga
     2221 cgcgctatgc actaaatttc cggcgtggac atccatatgc aatgggctta agcgtaaaag
     2281 ccgtggttcc cgcgctttgc gcagacgttt taacattcgc agccaaccca ctgtccgccg
     2341 cgccggatcg ctttgctgcc agtacagttc cagtaacggc aacagcgtta ttcgccagcc
     2401 aaaacgcggt tgttgatgta gataatacag caagcctgcc agttcgttgg tatccggcaa
     2461 atacgttttt acctcgccgg gcagatagtc gaccaccatc cagtcacgga gatataaatg
     2521 cggcttcggt gcaatgcatg cgggtagttg tgataaagcc cgatactggc gcaagaacgc
     2581 ggactgcggc gcatcaggat cgtgcggctg acgcacaaca aaacgctggc cctgatgttc
     2641 aatgagaaaa ctcccgccac taagcccact attaaacgtc gtgacgggat gaaactgcgg
     2701 gaaaaagcgc gacagcaatt cgtcgcgcgt gaggggatta ttgctgcgaa acggcacctt
     2761 tacctgacca gataatttcg cccgtctgca ccagcatcag ctgcatttgt agggtcggag
     2821 cgttaacgtt gccagaggcg ctggagtaca gcacgtaatg agcgccaaca ttgcgggcaa
     2881 tgcctatggc tttactacgg gtgcctaaac tgtcctgcgg cgacaaacct aattgttgct
     2941 tagccatcga cagctgctgg gcggaaacca gggtaaattt accgttatta gccagcgcat
     3001 ttcgcagcgt ttcggtcgct tctgcggcat tcaacgaacc attagtacgg ttgttaacgc
     3061 tatcaaccag caggacgcta cccgcagtca cgccgtcagc cccaagcatc ttactgacca
     3121 tcggctgcat tgcgccattc cagtcataat ggcgaatatg cggcgcaggc ggtgcagttt
     3181 gatcttcgtg ctcaattggg cctggctgct gcggtatcgt cggcaccgat ggcactgtgg
     3241 ggataggctg ttgtggctca gcaggttgtt ccggcgctgg tttcacttct tctaccggtg
     3301 caggttcacg ttgccccaca cacccggcaa gaaacatcgc cagcgcggta atcaaggcgt
     3361 agcgactcat ttttgtcatc aagattcacc ccttacaaat atagataaag tctgactttg
     3421 tgcgccccca gaaaattggc gctgccgtac agcgtgaccg ccgaatgtgc gggaatggta
     3481 acgctgcgtg gcctttccag aggatgcatc tccagccctc tggcgtcata ccagtaaaaa
     3541 cgataatgaa cggtaacggg ttcttgcctt tcgttataaa gcgttgagga tgctgaaggt
     3601 tgaatatcag atgtcgaaag gacgggcttt tctgcactga tgcccgcagc taataaagat
     3661 gactccatca ccagcgattg ctcatcattc accggaattt ccggatgtga acgacagccc
     3721 accagcacta acaacgccag agacatcagc ccaaagcatc cttttctcat cgttacagac
     3781 ctttatgcgc cagcattggt cccagcggac agccacccaa caaatgcata tggatgtggt
     3841 aaacctcttg tccgccatgg cggttggtgt tcatgatcag acgatagcca tcttcggcaa
     3901 taccttcttg ctcagcaatt tttgccgcta cggtgatcat gcgtcccagc gcctgctcat
     3961 gctcagctga cacgtcgttc acagtcggaa tgaggatatt cggaatgatc agaatatgcg
     4021 ttggcgcttg cggcgaaata tcgcgaaacg ccgttaccag atcatcctgg tagacgatat
     4081 cggaggggat ctcacgacga ataattttgc tgaatatagt ttcttctgcc acgacatttt
     4141 cctttttcaa atagccatgc gttgcgccat gctacgccgc gccacactgc gagtatagag
     4201 tatggtcgag ttaaacctgc tctttcaacc ttaactcacg atttcttaag caaaaaatcg
     4261 ttttgctgcc gtatatatcg ccattattcc catttctgcc tgcgaatgcg ctgaaaagac
     4321 attctctgtt tggtttacac actaaatatg aatgcgtata tttctcattt gcatttacat
     4381 acaaaattat tcgcacacta aatataaccg ctcatcaccc tgctccggca tgagcctgac
     4441 gccaacataa accaagagat ttcaaatgct ttcgacacaa tttaacaggg ataatcaaca
     4501 tcaagctatc atcaaaccgt cactccttgc cggttgcata gcactggcac tattaccttc
     4561 cgccgctttt gctgcaccag tcactgaaga aacggtgatt gttgagggtt cagccacagc
     4621 cccagatgat ggcgaaaatg attacaacgt aacgtctacc tctgcgggta caaaaatgca
     4681 gatgactcaa cgtgatattc ctcagtcggt cactattgtt agccagcagc ggatggaaga
     4741 tcagcagtta caaacgctgg gcgaagtgat ggaaaacacg ctggggatca gcaaaagtca
     4801 ggcggattcc gatcgtgctc tttattattc ccgcggattc cagatcgata actatatggt
     4861 tgatggtatc cccacctatt ttgaatcgcg ctggaatctg ggcgacgcac tttctgatat
     4921 ggcactgttt gaacgcgtag aagtagtgcg tggcgcgaca ggcctcatga ccgggacggg
     4981 taatccatct gcggcaatta atatggttcg aaaacacgcg accagtcgtg aatttaaagg
     5041 cgatgtctcg gcggaatacg gtagctggaa caaagaacgg tatgtggcgg atttacaaag
     5101 cccactcacc gaagatggta aaatccgcgc acgaattgtc ggcggctacc agaataacga
     5161 ctcatggctg gaccgctaca acagtgaaaa gaccttcttc tccggcattg tcgatgctga
     5221 tttaggcgat cttacgatgc tttcagccgg ttacgaatat cagcgtattg atgttaatag
     5281 cccaacctgg ggtggtttac cgcgctggaa tactgatggc agcagcaaca gttacgatcg
     5341 cgcacgcagt accgcacctg actgggcgta caacgataaa gagatcaaca aggtctttat
     5401 gaccctgaag cagcggtttg ctgatacctg gcaagcgacg ctgaatgcca cccactctga
     5461 agtcgaattt gacagcaaaa tgatgtatgt cgatgcctat gtaaacaaag cggatggtat
     5521 gctggttggg ccatacagta attatggacc tggttttgat tatgtcggcg gcaccggttg
     5581 gaacagcggc aaacgtaaag ttgatgcgct ggatttgttc gctgacggta gttatgaatt
     5641 gtttggtcgt cagcacaatc taatgtttgg tggcagttac agcaaacaaa acaatcgtta
     5701 cttcagttca tgggccaaca tcttcccgga tgaaattggc agtttctaca actttaatgg
     5761 caatttccca caaaccgact ggtcaccaca gagcctggcg caggacgata ccacacatat
     5821 gaaatcgtta tatgctgcca ctcgtgtcac ccttgccgat ccgctgcatc tgatcctcgg
     5881 cgcacgttat accaactggc gggttgatac gctgacttac agcatggaga aaaaccacac
     5941 cacgccttac gctggtctgg tgtttgacat caatgacaac tggtcgacct acgccagcta
     6001 tacctctatt ttccagccgc aaaatgatcg tgacagttca ggcaaatatc tgactccaat
     6061 caccggtaac aactacgagc taggtctgaa atcggactgg atgaatagcc gtctgaccac
     6121 cacgttagcc atcttccgta ttgagcagga taatgtcgct cagtccaccg gtacacctat
     6181 ccccggcagc aacggcgaaa ccgcctataa agcggtggat gggacagtca gtaaaggtgt
     6241 ggaatttgaa ctcaacggcg caattaccga caactggcag ttgacgtttg gcgcaacgcg
     6301 ctatattgcc gaggataacg aaggaaacgc cgttaatcct aatctgccac gcaccacggt
     6361 taaaatgttc accagctatc ggttgcctgt catgccagag ttgacggtcg gcggtggtgt
     6421 taactggcaa aatcgcgtgt ataccgacac cgtgacgccg tatggcacct tccgcgccga
     6481 gcaaggtagc tacgcgctgg tggatctctt cacccgctac caggtgacga aaaacttctc
     6541 gttacagggg aatgtcaata acctgttcga caaaacctac gataccaacg tggaaggttc
     6601 tatcgtctac ggcgcaccgc gtaatttcag cattaccggc acgtatcaat tctgaataac
     6661 acctgtaaaa aaggcagcca tctggctgcc ttagtctccc caacgtctta cggattagtg
     6721 gttacggatg tactcatcca tctcggtttt caggttatcg gatttagtac cgaaaatcgc
     6781 ctgaacacca gaaccagcaa ccactacgcc cgctgcgccc agtttcttca ggccagcctg
     6841 atccacttta gacacatcag caacgctgac gcgcagacgg gtaatacatg cgtcgaggtt
     6901 agtaatgttt tctttaccac caaatgcagc aaccagagcc ggtgccattt cgctggtacc
     6961 tgtcgctttt gcatcttcag tcgcgtcttc acgacccggc gttttcagat ccagtgcttt
     7021 aatcagcacg cggaagatgg tgtagtaaac aatggcataa ccgataccga cgatcgggaa
     7081 cagccacagt ttgctgctgt taccagacag aacgatgaag tcgatcagac cgtgcgagaa
     7141 cgacgtaccg tcacgcatcc ccagaagaat acagattggg aatgccaggc ctgccagaat
     7201 cgcgtggatg atgtacagga tcggcgcaac gaacatgaag gagaactcga tcggctcggt
     7261 gataccggtc aggaacgagg tcagcgccgc ggagatcata ataccgccca ctttcgcgcg
     7321 gttttctggt ttagcagagt gccagatagc aattgcggca gctggcagac cgtacatttt
     7381 gaacaggaag ccaccagaca gtttacccgc agtcgggtca cccgccatat aacgcggaat
     7441 gtcgccgtgg aaaacctgac ctgctgcgtt ggtgtattca ccaatctgca tctggaaagg
     7501 tacgttccag atgtggtgca gaccaaacgg taccaggcaa cgttcgatga aaccgtaaat
     7561 gccaaacgca actaccgggt tctggtaagc agcccactga gagaaggtct ggattgcaga
     7621 accaatcggc ggccaaatga aggacagcac aacgccagta aagatggcag ccaggcctga
     7681 aatgatcggc acaaagcgtt taccggcaaa gaagccaaga tactcaggca gcttaatacg
     7741 gtagaaacgg ttaaacatgt acgctgcgat cgcaccggag ataatccccc cgagtacgcc
     7801 agtatccgcc aggtgtttag aggcgatttc ttcagcaggt aaatgcagta ccagtggcgc
     7861 aaccacggcc atggttttaa ccatgatgcc ataggcaaca actgcggcca gcgcggatac
     7921 gccatcgtta ttggtaaagc cgagggcgac accgatcgca aaaatcagtg gcatgtttgc
     7981 aaagacggaa ccgcctgctt ctgccataac atgcgataca acggcgggca gccagctgaa
     8041 attcgcggaa ccgacgccca gcagaatacc tgcgataggc agtacggata ccggcagcat
     8101 cagcgattta ccgacctttt gcaggttagc aaatgcattc ttaaacataa ttgagagtgc
     8161 tcctgagtat gggtgctttt tttacgttct cacgcgtggc aaggggggag tgcctcgccg
     8221 tgtacagggc atctaagcgc cctttattta ttacacagag taaaataatt cagtgccgat
     8281 ttgtttgacg gctatcacgt ttcaacttca gatcattgga gaagttgcac attttgacaa
     8341 aacatatgtc acaatatttc aaacgtggtt attcaccgcc cgattttcgg cggtgaactt
     8401 tactcgtttt agccattaat tacgagcttt aaaaaaactc attcaacgga tggattgaag
     8461 gcgggaagcg tcgatgtgaa acagacgggc gaagttatcg gtggttacct gcgccagttc
     8521 ttcaacggca acacctttca acacagccat gtattctgca acgtcacgaa ccatcgcagg
     8581 ttgattctct tttcctcgat gcggtactgg cgcaaggtaa ggtgagtcag tttccaccag
     8641 taaccgatcc agggggacat aacgcgcagc atcgcgcagt tgctccgcat tacggaaggt
     8701 cacaatgccg gaaaaggaga tgtaaaatcc gagatccagt aatttgcccg ccgtttctct
     8761 gtcctctgta aaacagtgta gtacgccacc gcaatccgtc actttttctt cgcgcagaat
     8821 tgccagcgta tcggcgcggg cgtcacgggt gtggacgata accggcttgt tcagttcacg
     8881 accaatctgg atatgatgga tgaaggactc ttgctgacgt actttagttt ccggcgtgta
     8941 ataataatcc agcccggttt cacccagcgc tacaacgccc tcttctgccg ccagacgacg
     9001 taaatcttct acatcgtagg gatcattctg gttgagtgga tgcacgccac aagaaaatac
     9061 gacgttgtcg cgttcgccta ccagatcccg catgtgtaaa taacccggta atgttgtggc
     9121 gactgccaga caaaatttca catcgcgtgc ggcggctttc gccagaacgt catccacgtc
     9181 cttatgcaaa gattcataat ccagaccatc gagatggcag tgtgagtcga ctaaaaacat
     9241 gatgtctctc ttaaagatga ggaaccggta gcacaacgcc cggttgcagg taatgctcaa
     9301 tacgcagtaa aagatcggtg atgagaagct cgcggttgat acctgtgaca gacattaact
     9361 gttcacgaat gtggcaaaca tcccccagta tagcctgcag gcgcgaggga gaaagatggt
     9421 ttgccagttc gacgaccagc cccggcacat caacattggt cacgtgcgca gcgccataat
     9481 ggcgttttag cgcatccatc agcaacgttg ccagccagtg taaacgcgcc ggagcttgtt
     9541 catgattaag ggccgccagc agcgaatacc agtcgcccga ttgcacgcta tatgccagcg
     9601 cctgacacaa tgtttcacga gcctgccagt tatctccctg aaacaacgcc agtgccgccc
     9661 caggcgaacc ggcgcttaag cgcaatgcgg caagtagtgc atcctgtgac attgtcactt
     9721 cgcgtgaaag ccaggtcacg gcgtactgtt ccggcggcgg cgcaaggtaa tgtaaccgac
     9781 aacgactacg taatgttgcc agtaaacgtt ctggctcgcg ggtagcaagg aaaaaccacg
     9841 tctctgctgg tggctcttca agcgttttca gcaatgcgtt agccgcggcg tcggttagta
     9901 aggcagcatc ggttacccag acaactttcg caccacctaa gcgtgcgtgc tcattcagct
     9961 tttcggtgac ctcacgtacc gcatcaatgc ccagcgcatt tttccctttt tcgggagcca
    10021 gggtgtagta atcgggatgt gtgccagcct gcatcaactg acatccacga cagtgaccgc
    10081 aacttttgtg gccctgcggt tgttggcaga gtaaataacg gctcagggcg tagattaaag
    10141 catcatcgcc catgcccggt aacgcctgaa tgagtagcgc atggtgacct cttccggcct
    10201 gatagctggc taccagtttt tcgaaatcag gtcgtaacca tggataccat ctcatgcgtc
    10261 caactccttc acccagttgg tcacggtagt gcggattgca tccatcacgg cctccagcgg
    10321 ctgggtggca tcaatggtat gaatgctttt atcttgtgct gccagttcca gatagcgggc
    10381 gcgggtgcga ttaaagaaat cgaaagattc ttgctcaata cgatccagct cgccgcgcgc
    10441 acgcgcgcgt tttaagccaa cctccggggt aacatcgaga tagagcgtta agtcagggcg
    10501 aaaatccccg agaacagcat cacgcagtgt tgccagcata tgttggtcaa tacctcgtcc
    10561 tccaccctga taggcttgag tagagagatc gtggcgatcg ccaatcaccc aggtgccgtt
    10621 agccagcgca ggtttgatga ccgtttctac cagttgaacg cgcgcggcat aaaacatcag
    10681 aacttcggct ttatcggtaa tgacttcatc gcctaccgat ttgatatcca gcaccaggct
    10741 tcttaacttt tcggcaagtt gcgtaccgcc aggttcccga gtgaaaacca tgtcgcggat
    10801 acccagttgc tcaagcgtct caaccaccac gttacgcgcg gtagttttgc ctgcgccttc
    10861 cagcccctca atgacgatat acttactgcg catttttttc cttaagcact ttcagataat
    10921 cctgcacaga cttgttatga ctggcaagat tggtattaaa cgtgtgacca cctttaccat
    10981 cggccacaaa atagagatac ggcgtttttg ccggatgcgc agcagccttc agcgaatccg
    11041 cccccggcgt agctattgca cctggcggca aaccggtaat ggtataggtg ttatacgctg
    11101 tcggcgtttc caggtctgca cgagaaagtt tgccattata acgctctccc atcccgtaaa
    11161 tcacggtcgg gtcggtctgc aggcgcatac caatgcgtaa ccggttgata aataccgagg
    11221 caacctgatc gcgttcactg gcaacggcgg tttctttttc gataattgat gccatcgtca
    11281 ccaactggtt tttatcttta taaggcagac cgtccgcacg cccttcccag gcgctatcga
    11341 ccgctttcac cattttcttg tgcgctcgct tgagtaacgc gacatcggtg gtattggcgg
    11401 tatacatcca ggtgtctggc cagaaccaac cttcaatcca ctccgggttt tccagttcaa
    11461 gtgcctgcgc tacggtggcg tacttatcgt cgctcagcgt gtgcttgata tacggagcct
    11521 cacgcaattg cttgaggtag tcgctcagac gcatcccttc taccagtcgc agcgggaact
    11581 gtgcttcttt accgctttcc aacaatttca gcatctcgcg cacggtcatc tgcggtgtaa
    11641 agcggtaagt cccggcttta aagtgagaaa gatccggttc gatacgcagc aaccattgaa
    11701 aaacccgtgg gcgattgatg atcttatcgg cataaagctg ttcacctagc gccaaacgtc
    11761 cggtccctgg cttcagggta aatatcgtct cttctttgat aagcaatttg ctgtcggcaa
    11821 gatggcgaac cttccagacg cccacaccag cggcgatacc cagtaccacc agcaataaca
    11881 aaattatcaa taacactttt ttcatgacta attcgggcgc tcacaaagtg gggctaaata
    11941 ttcatataac gttgctgacg aaaaggagac atcgccacag gcacgcacgg gcatcactgg
    12001 cattaacgca ttacaaataa ccatctcatc tgcctgcaac gcctcttcca gagaggcttg
    12061 cacttcgaca agctgataag aggattgtgc cagcaaacgg atacagaatt gtcgcataat
    12121 gccgttaaca cctgcctgat ccagtcgcgg cgtataaact acgttgccct tccgccagaa
    12181 caaattagcc gcacagcatt ccgtaaccca cccttcgctg tcaaggacca gcgcctcatc
    12241 agcgtttgtc tgctcaagat gagagcggat caatacttgc tcaagccgat taagatgttt
    12301 aatacctgca aggtgaggat tgcgccccag ccgcaccggg cttagcgcca acatcatccc
    12361 ttcgttacgc aaacggtcgt aatgtgcagg ataagccgta atggagagaa tccgcgttgc
    12421 cggtccactg ttcaatgtgc tgtaccctcg cccgccacta ccgcgactga tcacgacttt
    12481 cagcacacca ttctgctgtt ctgctgccag cgttttcatc tcttgttcaa gctgaggcca
    12541 gaagtcacag gaaatcatca accgctgaca agcatcctgt agtcgctgga tatgcgccga
    12601 taacaaactg actttaccgt cgataactct ggcggtggta aaacaaccat caccaaactg
    12661 cgttgcccga tcgcttactg ccagcgattc ctgcttatga ccgttaatta agaacatacc
    12721 agctccttat catagaattg ttagtgtggc agcatgttca ctacggaaca agtcggaata
    12781 aaagctaaga aaaaaggccc gcaagcggac cttttataag ggtgggaaat gacaacttag
    12841 atctttttaa agatcaaaga accattagtg ccaccgaagc cgaaggagtt acacagcgtg
    12901 tattccattc cgctgacctg acgtgcttcg tgcggtacga aatccagatc gcaaccttca
    12961 tccgggttat ccaggttgat ggttggcgga acagcctgat cgcgcagcgc caggatggag
    13021 tagatagatt ctactgcacc cgccgcacct aacaggtgac cggtcataga tttcgtggag
    13081 cttaccaaca cacggcttgc agcttcaccg aagatggttt tcaccgcctg cgcttcagct
    13141 ttatcgccag ccggcgtaga agtaccgtgc gcgttaacgt agccaatctg actcgcttca
    13201 atgcctgcat cacgcagagc atttgccatc gccagagctg cgcctgcgcc attttctggc
    13261 ggtgacgtca tatgataagc atcgctgctc ataccaaagc cgacgagttc agcgtaaatt
    13321 ttcgcaccgc gttttttcgc gtgttcgtac tcttcaagta ccagcatacc ggcaccatcg
    13381 cccagtacga aaccatcacg ctctttatcc cacgggcggc tcgccgcttg cgggttatca
    13441 ttgcgggtag ataatgcacg tgccgcgcca aaaccaccaa cgcccagcgg cgtactggct
    13501 ttctctgcgc cacctgcaac catcacgtca gcatcgccat acgcgataat acgcgcagca
    13561 tggccaatgt tgtgcacgcc ggaagtacag gcggtcgcga tagagatgct tgggccacgc
    13621 aggccataca tgatagtcag atgacctgcc accatgttca caatcgttga cggaacgaag
    13681 aatgggctga tcttacgtgg accaccgttc atcagagatg tgtggttttc ttcgatcagt
    13741 ccgaggccgc caatcccgga gccaattgcg gcaccaatgc gggttgcgtt ctcttccgtt
    13801 atttcaaggc cagaatcctg catggcctga acgccagcga caattccata ttgaatgaag
    13861 gcatccatct tgcgctgttc tttgcgcgag ataatgtcct cacagttaaa atcctttact
    13921 aagccagcaa atttcgttgc ataggcgcta gtatcgaaat ggtcgattag gctgatgcca
    13981 ctctgaccgg caagcagagc tttccaggta gactctacgg tattgccgac aggagacaac
    14041 atgcccagtc cggtcacaac tacacgacgc ttagacacgt ttgtcctcca gggagggaaa
    14101 aaatgattct agtgggacaa aaagataaaa ctcaggcggt cgaacgaccg cctggagatg
    14161 ttcacttacg cctggtggcc gttgatgtaa tcaatggcag cctgaacggt ggtgattttc
    14221 tcagcttctt cgtccggaat ctcagtatca aactcttctt ccagagccat taccagctca
    14281 acggtgtcaa gagaatccgc gcccaggtct tcaacgaaag aagcattgtt ggtaacttct
    14341 tcctgcttaa cgcccagctg ttcgccgata attttcttaa cgcgttcttc gatagtgctc
    14401 atactcttaa atttcctatc aaaactcgct ttcgcgatgg ttttcgtagt gtataaaatg
    14461 ttgaaaaatt tgcaactaaa tcccggcagg tcttaccacg attttacgtt attttgaggc
    14521 cttacccccc aataacgcaa atcattttgc actaattatt actcataacc acgcagactg
    14581 cgcgcaacat gagcattttg tgcaaatcgc ggtcagacca tgtacatccc gccgttcaca
    14641 tgcaaagttt cacccgtgat gtaagctgct tcgtcggatg ccaggaatgc aaccgcgttg
    14701 gcgatttcct gtgcgccgcc gaggcgaccc gcaggaacct gcgccaggat acccgcacgc
    14761 tggtcatcgc tcagcgcacg tgtcatgtcc gtttcaataa agcccggagc aacaacgttt
    14821 acagtaatac cgcgtgacgc aacttcgcgc gccagtgatt tactgaagcc gatcaagccc
    14881 gctttcgccg cagcgtagtt ggcctgaccg ccatttccca tggtaccaac cacagaaccg
    14941 atagtgataa tacgaccatg acgctttttc atcatagcgc gcattaccgc ttttgacaga
    15001 cggaaaacag atgaaaggtt ggtttcgata atatcgttcc actcttcatc tttcattcgc
    15061 attaacaggt tatcacgagt gataccggca ttattgacca ggatatccac ttcaccaaat
    15121 tctgcgcgaa ttttttccag aacagattcg atagatgccg ggtcggtcac attcaacatc
    15181 agacctttgc cgttagcacc taaataatca gtgatcgcct gagcgccatt ttcactggtc
    15241 gcagtgccaa taactttcgc gccacgggct gcgagcgttt cagcaattgc gcggccaatt
    15301 ccgcggcttg caccggttac cagtgcgatt tttccttcaa aattcatgat tttcctcttt
    15361 taaagctcga gcgccgctgc catcgctgaa ggttcgttca gcgccgaggc ggtcagggtg
    15421 tcgacaatgc gtttcgtcag gccagtaagc actttgcccg ggccgacttc atagagatgt
    15481 tctacgcctt gcgctgcaat gtactcaaca gacttcgtcc actgaaccgg gttatacaac
    15541 tgacgtacca gtgcgtcacg aatggcatca ccattggttt cgcatttcac atcaacgtta
    15601 ttcacaacag gaactgttgg tgcgttaaag gtgattttcg ctaattctac tgccagtttg
    15661 tcagctgctg gtttcatcag cgcacagtga gatggtacgc tcactggtaa cggcagagcg
    15721 cgttttgcgc ccgcagcttt acaggcagcg ccagcacgct caaccgcttc tttatgaccg
    15781 gcaataacca cctgtcccgg agagttaaag tttaccggag aaacgacctg accttctgca
    15841 gcttcttcac acgcttttcc aatagacgca tcatccagac cgatgattgc cgccatagcg
    15901 cccgttcctt ccggtacggc ttcttgcatg aacttgccgc gcatctcaac cagacgcacc
    15961 gcatcagcga aatcaatcac accagcgcaa accagcgcgg agtattcccc caggctgtga
    16021 ccggccatca ttgccggtgc tttaccgccc tgctgctgcc atacgcgata cagcgcaaca
    16081 gatgcagtca acagcgctgg ctgggtttgc caggttttat tcagttcttc agctggcccc
    16141 tgctgggtca gcgcccacag gtcgtagccc agcgccgcag aagcttcagc aaacgtttct
    16201 tcgacaattg gatagctcgc cgccatatca gccagcattc caacggtttg agaaccctgt
    16261 ccagggaaca caaatgcaaa ttgcgtcatg ttttaatcct tatcctagaa acgaaccagc
    16321 gcggagcccc aggtgaatcc accgccaaag gcttcaagca gaaccaactg tcccggctta
    16381 atgcgcccgt cacgtacagc ttcatccagc gcgcacggga cagaggccgc agaggtatta
    16441 ccgtggcgat ccagcgtcac cacaacattg tccatcgaca tgccgagttt tttcgccgtt
    16501 gcactgataa tacgcaggtt agcctgatgc ggaaccagcc agtccagttg agaacggtca
    16561 agattattcg ccgctagcgt ctcatcaacg atgtgcgcca gttccgttac cgcaaccttg
    16621 aagacttcgt tacccgccat tgtcagatga attgaattct ctggattcac acggtcagca
    16681 ttaggcagcg tcagcaattc accatagcta ccgtcggcat gcagatgggt ggagatgatt
    16741 cccggctctt cagaggcagc cagcaccgca gcgcccgcgc catcgccaaa aataataata
    16801 gtcccacgat cggttggatc gcaggtgcgc gccagtacat cggaaccgac gaccagagca
    16861 tacttcaccg ccccagattt cacgtattga tcggctacgc ttaatgcata ggtgaaacct
    16921 gcgcaggctg ctgcaacgtc aaatgccggg caacctttaa tgcccaacat gctttgaatc
    16981 tgacaagctg cgctcgggaa agcgtgcgtc gcagaagtcg ttgccacaac gatcaggcca
    17041 atctggtctt tctcaatacc cgccatctca attgcgcgtg tcgccgcttc aaagcccatg
    17101 gttgaaacgg tttcgtttgg cgcggcaatg tggcgttcgc ggataccggt acgagtgaca
    17161 atccactcgt cagaggtatc caccattttt tccaaatcgg cgtttgtccg cacttgttcg
    17221 ggcagatagc tgccagtacc aataatcttc gtatacatgt acgctcagtc acttttcggt
    17281 tatataccgt cacttgcaaa ctgcgagttc gctggcagcg tcctgctacc gcagagttcc
    17341 gcttttgcca ccgtccagca gctcaaaacc agctgggtat acagattcca ggcgagcggc
    17401 aattcgctga ggaacttgtc gctgcaccgc ctgcactgcc tgttcaatcg cgaccgcaaa
    17461 agctcgctga ttggctgcac catgactttt tatcaccgtg ccgcgcaatc ctaacagaca
    17521 ggcgccgtta tactggtcgg ggttgaggtg actgaatcgc ctcgtcaggc tcttttgtag
    17581 ccaacgcttt aataacagta gccaccacga ccgttttttc ccttcaccct gagatttcag
    17641 cagagaaagg aacatcctga caacaccttc catcgtcttt aatgtgacat ttcctgtaaa
    17701 gccgtcacaa accagcacat ctgtcttgcc agttaacaac tcattggctt caagatagcc
    17761 gatataattg atagaaggga ttgttttaag caccgctgag gcatcccgaa tactgtcgag
    17821 accctttact tcttcttcac caatattgag caacgccacg cgaggattag gaatttccac
    17881 cacctcttca gccagaactg agcccataat ggcaaattgc accaacattg tactgtcaca
    17941 atcgacgttg gcccctaaat cgaggaccac cgttttgccc ttttgctgat gcggtaatac
    18001 cgtcaccagc gccggacgct caatcccctc caggggcttg agtaataatt ttgccagccc
    18061 cattagcgcc ccggtattac cggcactgac acaggcttgc gctcgacctt ctttcaccag
    18121 ctccagggcc acgcgcattg aactcccacg actggcgcgg atagcttgcg aaggccgggc
    18181 atcactggcg ataactgact gcgcaggaat aatctgcaga cgcgaacgtt gttcaaagtc
    18241 agctttagca agtaatggcg tgatggcgtc gggattgccg actaaaagaa gagtgagttg
    18301 cgaattagaa ttcagtgcct gcaatgctgc aggcactgtc acggaagggc caaaatcccc
    18361 tcccatgaca tctaacgcca gggttagacg tgtcaaggta tcgtcgccgc ccggtttggt
    18421 ctttccccag ttgcctgggg aaaatcctca ctaagcttca tcacgcagac gcgtgattac
    18481 ttagcgatga ccttgcggcc gcggtagtaa ccgtcggcag tgatgtggtg acgcaggtgt
    18541 ttttcaccag aagttttgtc tacagacagg ctggtgactg cggtcagcgc gtcatgggaa
    18601 cgacgcatgc cacgtttgga acgggttggt ttattctgtt gtacggccat ggaccttact
    18661 cctcaattac ggtttatccc cgatccaacg gggagcacca attacttacg ctttaagctg
    18721 gctaatacgg caaatgggtt tggcttttgc gcttcttcag gcagttcacc aaagaccatg
    18781 tccgcttcgg acacttcaca gtgttcagaa tcatgcaccg gaactaccgg caaggcgagg
    18841 atgatttcat cttcaaccat tgcaagcaga tcgatttcac cgaattcgtt aacctcaatc
    18901 ggttcatacg cttccggcag tgcttcagcc tgttcgtctg aacgcacagg actaaaacaa
    18961 tacgttgtgt agacctgatg agtaaacggc ttcccgcaac gctgacactc gagcgttacc
    19021 gtcaccttcg catcgccgtt taacactgcg agacgttggt tatcgatagc gaacgacatg
    19081 gagcattcca catcactgtc cacactgact acggattcgg cgacgcgctc aacctgatca
    19141 ggagtataga taccctggta atcaaggcgt ttctgagccg tacgaaccgg atcgagagtc
    19201 aggggtaatt ttaccttttg catagggcgc gcatattaac tttgtaacgt catagagtca
    19261 aagaaaaagg cagcctgtgg ttgccttttg ccaataattc gcacacattg cgggttactg
    19321 ctttattttc gttcagtgct gataacgaac atcaacctgc aaaacgaagc gcatagttta
    19381 aaatggctat catcaatacg ctatatctga tggaaaaaat atgcctaaac ttattttagc
    19441 ctccacctcg ccctggcgtc gcgctcttct ggaaaaactg caaatctctt ttgaatgtgc
    19501 agcacccgag gtcgacgaaa ctccacgcag cgacgaatca ccgcgacagt tggtgcttcg
    19561 actggcacaa gaaaaagcgc aatctctggc gtcacgttat ccggatcatt taattattgg
    19621 ttcagaccag gtatgtgttc ttgatggcga aatcaccggt aaaccgttaa cggaagaaaa
    19681 tgcccgtctg caattacgca aagccagcgg caatatcgtc accttctata ctggactggc
    19741 gttgtttaat tcggcgaatg ggcatctaca aacagaagtg gagccttttg acgtccattt
    19801 ccgtcacctg agcgaggcgg agattgataa ttacgtgcgt aaagagcatc ccctgcactg
    19861 cgcgggtagc tttaagagtg aaggatttgg cattacgctg tttgagcgtt tagaggggcg
    19921 tgatcctaac acgctggttg gtttaccgct aatcgcgctg tgtcagatgt tacgtcggga
    19981 agggaaaaac ccgctgatgg gatagttttt tgcgccggat gcggttactt cgcatccggc
    20041 aatcggtgct ggcggcctga tgcggcgtaa acgccttatc aggccaaccg cccattatca
    20101 gatttatatg cttgtattag cgcgcgttac gcagcttttg caaacaacgc ttcaaacctt
    20161 catccatcgg cgcttcaata cgcatcacct caccggtccc cggatgagta aacttcaacg
    20221 ctgcggcgtg caggaacagg cgatttaatc ccgtgcctgc ttcagtgagc tgtctgtcaa
    20281 attcacggtc accgtagcga tcgtcaaagg caatcggatg acccgcatat tgtgtatgca
    20341 cacggatctg atgagtacgc cctgttaccg ggctacaacg caccagggtg gcaaatgcat
    20401 agcgctcttc cactttaaag cgtgtttccg acggtttgcc ttcctgactc acacgcacga
    20461 tacgttcgcc gctttgcaga atatttttca gtaacggcgc ttgaacgctc ttcacatgcg
    20521 actgccactg accgcgcacc agcgccaggt aatctttttg catccctttt tcacgtaatt
    20581 gctcatgcag agaacgcaac gccgagcgtt ttttcgctac cagcaacaca cctgaggtgt
    20641 cccggtcaag acgatgaacc agttcaagga accgcgcttc cgggcgcaac gcccgcaaac
    20701 cttcaataac gccgaagctt aaaccactgc cgccatgtac cgccgtaccg gaaggtttat
    20761 tcagcaccag gatatgatca tcttcatata agatgacgtc cgccagcgcc gccacctttt
    20821 gcagatgtgg cgaaaccgcc tcttcttccc gctcagcaac gcgaaccggt gggatacgca
    20881 cctcatcacc cgcttcgagt ttatattcag gcttaatacg ttttttgttc acccgcactt
    20941 cgcctttacg caaaatacgg taaatcatac tttttggtac gcctttcaat tgggtacgca
    21001 aaaagttgtc gatacgttgc cccgcttcgt cagcggtgat agcaacaatt tttacggatg
    21061 gagtctctgt tttcatggtg ggcgattcta attagccaac aggattcgcg ccactcattt
    21121 ttctatgctt atatttagtt ttacacctta ttacttcact aggtgatcac tttattgatg
    21181 gttattaaac caatcaccag caagaagtga aaaaactgtg agtaagcggg tgataaatgg
    21241 taaaagtcat cttgctataa caaggcttgc agtggaataa tgaggccgtt tccgtgtcca
    21301 tccttgttaa aacaagaaat tttacggaat aacccatttt gcccgaccga tcatccacgc
    21361 agcaatggcg taagacgtat tgatctttca ggcagttagc gggctgcggg ttgcagtcct
    21421 taccggtaga tggaaatatt tctggagagt aatacccagt ctgtttctct gataattgcg
    21481 ctgtttttcc gcatgaaaaa cgggcaaccg acactctgcg cctctttgag ctgacgataa
    21541 ccgtgaggtt ggcgacgcga ctagacacga ggccatcggt tcacacccgg aaaggcgtta
    21601 ctttgcccgc agcttagtcg tcaatgtaag aataatgagt aagttacgat gaaaagaatg
    21661 ttaatcaacg caactcagca ggaagagttg cgcgttgccc ttgtagatgg gcagcgtctg
    21721 tatgacctgg atatcgaaag tccagggcac gagcagaaaa aggcaaacat ctacaaaggt
    21781 aaaatcaccc gcattgaacc gagtctggaa gctgcttttg ttgattacgg cgctgaacgt
    21841 cacggtttcc tcccactaaa agaaattgcc cgcgaatatt tccctgctaa ctacagtgct
    21901 catggtcgtc ccaacattaa agatgtgttg cgtgaaggtc aggaagtcat tgttcagatc
    21961 gataaagaag agcgcggcaa caaaggcgcg gcattaacca cctttatcag tctggcgggt
    22021 agctatctgg ttctgatgcc gaacaacccg cgcgcgggtg gcatttctcg tcgtatcgaa
    22081 ggcgacgacc gtaccgaatt aaaagaagca ctggcaagcc ttgaactgcc ggaaggcatg
    22141 gggcttatcg tgcgcaccgc tggcgtcggc aaatctgctg aggcgctgca atgggattta
    22201 agcttccgtc tgaaacactg ggaagccatc aaaaaagccg ctgaaagccg cccagctccg
    22261 ttcctgattc atcaggagag caacgtaatc gttcgcgcat tccgcgatta cttacgccag
    22321 gacatcggcg aaatccttat cgataacccg aaagtgctcg aactggcacg tcagcatatc
    22381 gctgcattag gccgcccgga tttcagcagc aaaatcaaac tgtacaccgg cgagatcccg
    22441 ctgttcagcc actaccagat cgagtcgcag atcgagtccg ccttccagcg tgaagttcgt
    22501 ctgccatccg gtggttcgat tgttatcgac agcaccgaag cgttaaccgc catcgacatc
    22561 aactccgcac gcgcgacccg cggcggcgat atcgaagaaa ccgcgtttaa caccaacctc
    22621 gaagctgccg atgagattgc tcgtcagctg cgcctgcgtg acctcggcgg cctgattgtt
    22681 atcgacttca tcgacatgac gcctgtacgc caccagcgtg cggtagaaaa ccgtctgcgt
    22741 gaagcggtgc gtcaggaccg tgcgcgtatt caaatcagcc atatttctcg ctttggcctg
    22801 ctggaaatgt cccgtcagcg cctgagccca tcactgggtg aatccagcca tcacgtctgc
    22861 ccgcgctgct ccggtaccgg taccgtgcgt gacaacgaat cgctgtcgct ctctattctg
    22921 cgtctgatcg aagaagaagc gctgaaagag aacacccagg aagttcacgc cattgttcct
    22981 gtgccaatcg cttcttacct gctgaatgaa aaacgttctg cggtaaatgc gattgaaact
    23041 cgtcaggacg gcgtacgctg tgtgattgtg ccaaacgatc agatggaaac cccgcactac
    23101 catgtgctgc gcgtgcgcaa aggggaagaa acctcaacct taagctacat gctgccgaag
    23161 ctgcatgaag aagcgatggc gctgccgtct gaagaagagt tcgctgaacg taagcgtccg
    23221 gaacaacctg cgctggcaac ctttgccatg ccggatgtgc cgccagcacc aacgccagct
    23281 gaacctgccg cgcctgtcgt agccccagca cctaaatctg caccggcaac accagccgct
    23341 cctgcccaac ctgggctgtt gagccgcttc ttcggcgcac tgaaagcgct gttcagcggt
    23401 ggtgaagaaa ccaaaccgtc cgagcaacca acaccgaaag cagaagcgaa accggaacgt
    23461 caacaggatc gtcgcaagcc tcgtcagaac aaccgccgtg accgtaatga gcgccgcgac
    23521 cctcgtagtg aacgtactga aggcagcgat aatcgcgaag aaaaccgtcg taatcgccgc
    23581 caggcacagc agcagactgc cgagacgcgt gagagccgtc agcaggcaga ggtaacggaa
    23641 aaagcgcgta ccaccgacga gcagcaagcg ccgcgtcgtg aacgtagccg ccgccgtaac
    23701 gatgataaac gtcaggcgca acaagaagcg aaggcgctga atgttgaaga gcaatctgtt
    23761 caggaaaccg aacaggaaga acgtgtacgt ccggttcagc cgcgtcgtaa acagcgtcag
    23821 ctcaatcaga aagtgcgtta cgagcaaagc gtagccgaag aagcggtagt cgcaccggtg
    23881 gttgaagaaa ctgtcgcagc cgaaccaatt gttcaggaag cggcagcccc acgcacagaa
    23941 ttggtgaaag ttccgctgcc agtcgtagcg caaactgcac cagaacagca agaagagaac
    24001 aatgctgata accgtgacaa cggtggcatg ccgcgtcgtt ctcgccgctc gcctcgtcac
    24061 ctgcgcgtaa gtggtcagcg tcgtcgtcgc tatcgtgacg agcgttatcc aacccagtcg
    24121 ccaatgccgt tgaccgtagc gtgcgcgtct ccggaactgg cctctggcaa agtctggatc
    24181 cgctatccaa ttgtacgtcc gcaagatgta caggttgaag agcagcgcga acaggaagaa
    24241 gtacaagtgc agccgatggt gactgaggtc cctgtcgccg ccgctgtcga accggttgtt
    24301 agcgcgccag ttgttgaaga agtggctgaa gtcgtagaag cccccgttca ggttgccgaa
    24361 ccgcaaccgg aagtggttga aacgacgcat cctgaagtaa ttgccgccgc ggtaactgaa
    24421 cagccgcagg tgattaccga gtctgatgtt gccgtagccc aggaagttgc agaacacgca
    24481 gaaccggtag ttgaaccgca ggaagagaca gcagatattg aagaagttgc cgaaactgct
    24541 gaggttgtgg ttgctgaacc tgaagttgtt gctcaacctg ccgcgccagt cgtcgctgaa
    24601 gtcgcagcag aagttgaaac ggtagccgcg gttgaaccag agatcaccgt tgagcataac
    24661 cacgctaccg cgccaatgac gcgcgctccg gcaccggaat atgttccgga ggcaccgcgt
    24721 cacagtgact ggcagcgccc tacttttgcc ttcgaaggca aaggtgccgc aggtggtcat
    24781 acggcaacgc atcatgcctc tgccgctcct gcgcgtccgc aacctgttga gtaataatta
    24841 gctcaagtaa tcaagccctg gtaactgcca gggctttttt atttcagggg tagcatcttt
    24901 gctgtaatga cattagcgtt acatgtgctg tataattatc atccatgcaa agccgaagga
    24961 tatatttatg ccagcagcca ataacattcc aaccaggcgc gaatctttaa atttgcgcat
    25021 aaaacccgca gaacgcgatc ttattgaccg ggccgctaaa gcccgaggaa aaaatcgcac
    25081 cgaatttgtc cttgaggccg ccagagccgc agctgaagag gcgctcattg agcagagaat
    25141 tttcaggatc gattctgatg attaccagct atttttgaat cgtctggatc agccccccgc
    25201 gcccaatgct gcattacgca aaaccatgca aacacccgcg ccgtgggagc aaaaaaaatg
    25261 attcaggccc ccgagccact catggcaagg catcaattca cctctttttg ctcgggagta
    25321 gaaacgatgg ataactggct aaagcagaga gccctaaaaa atcagcttgc cggcgcatca
    25381 cgtacatttg ttagctgtga tacgtatagc aacgttttgg catattattc gcttgcttcc
    25441 agcgccgttg aaacgtatgt cgcgacagga cgatttcgcc gaaatatgcc agaaccgatc
    25501 cctgttgtag tacttggtcg tctggcaata gataaatcat tacagggtca gggcatcggt
    25561 cgtgcgatgg tcagagatgc cggtctacga gtactacagg cggctgaagt cattggtatt
    25621 cgcggaatgt tggtccatgc gttatcagac caggcccgtg agttttatct gcgagtgggg
    25681 tttgaaccat cgcctgtcga ttctatgata ctgatggcaa cattggctga tttgcaggaa
    25741 tgtcttaaat agttcgttat gtctcatgca aaaaaatgcc cgccccggcg atcccagagc
    25801 gggcagacaa aacataccca gtttcatgat atgttttaaa gcgagcttat ttgttgagct
    25861 ggaagagcga caatccctgc atatcggtaa atgctttata cgatgcctgc aatgccgttt
    25921 gctgcatgat gtaagatgaa atagttgcat tccagtccac atcaaccaga tcgctcatct
    25981 gctgcgtttg ccctaaagcg cgatcgctac ctaatgaatc aagcgactcc agttcgttca
    26041 gctgcgtgcc taattccgcg cgaacagtca gcacattgtt cagcgagttt ttcagtccgc
    26101 ggttggtttt atccagtgcc gcagcggcgg tttctttatc cgcttcgcta tccgcgaccg
    26161 gcgttttcag ggctgcgatg gcgctatcca gcatggcaaa aagattggtt tcagaagcgc
    26221 taccgtctgg ttccgctacc gcgttgctgg taatactgtc gaaaattttg tcgcccgtat
    26281 gtcctatcac catcgaacgc gaagcatcga cctgttgttt aatactttct gttccgccga
    26341 cataatcacc gtcagcctca ctaaacggcg cagtctctgt tttataaccg gcaaaaatgt
    26401 agcgcccgtt accgtcagtg gtgttcgcca gattcagtaa ctgatcacga agcccctgaa
    26461 tatccgtagc cagtgaggcc cggtcatcgt cactcaaggt gccattgctg gcgtagacaa
    26521 ttttttcctg agcattctgg atagcagtgg tgacctggct aagcacactc tcttccagtg
    26581 acactttttg agtggcgaaa gtacgcgcca gcgtgtactg gctgttttgt gcctgtgcct
    26641 gggagagaac aacagcttgt gatgcagcaa tgggatcatc tgaagggtta acgactcgct
    26701 tacccgtcga catctgttcg ccgtacttca tccattctgc ctgagaattg gtgatgccac
    26761 gcatgttttg ctggtacatc atctgggtac tgaaacgcat tttatctccc cttagcgaat
    26821 gttaatcagc gcatcaaaaa tcgcgtttgc cgtctgcaga acctgcgcat tcgccaggta
    26881 atactgctga aaacgttgca gatttccgta ctcctcatcg agattgacac cggaaatcga
    26941 ctgctgctga ttggaaagct gcgtcaccac attaccttgc gtggcgctac tggttttcaa
    27001 cgtcgcggtt ttattcccga tatcactcac taatgaggca taagcgtcgt taaaggattt
    27061 cgcaccgccc accgttttac tgttgctttg cagatccagc agggcctgac cgttgcggtt
    27121 gtcgctatca cccgcatctt cttcgctcgc catggcgatt ttcgcttcgt cagtgattaa
    27181 tacatccatg ttgacgatgg cgtcacttac cggtttcagc gtgaagctgt cgttaacggc
    27241 aggcgttcct gtaaacgtca actccagacc atcaaatgcc actttaccgt tggcatccgg
    27301 cgtcactgta aaagtggtat tgctggcaag gcgggtgacc tgccactgat tattatcgaa
    27361 cgagattttg taatctgtcg ccagtaccgc ggaggcatca gttaccgtgg caccgatcgc
    27421 aacgtcaccg ttgtttttcg tgttttgcag aaccgcgggc ttaccgatag caaagaaatc
    27481 tttaccggca tcgccgttgg catcaaatcc ggctttgtgt tgggtgttga aagcctcggc
    27541 aaatgccagc gccagttgtc cgagcgtatt acgcgtctgg tccagatcca gagaacggaa
    27601 tgtcagaatg ccgcccagcg acccggtatt cagtaatttc tccgggatct caatattgcc
    27661 agccgtccca tcaatataag cgacagtcgt acgagaaggg tcggcgctgg aaggaactgc
    27721 cgccagctgc cgcgccgtac ttccctgaac cagtgagtag ccattggcca tcgtgatgtt
    27781 ataagtgcca ccatcctgaa cgctaacttc tacgccaaca atctggttta attcactcac
    27841 cagttgatcg cgttgatcca gcagattgtt aggtgacgcc cctgccccca cgcctgtcag
    27901 acgcgagatt tgatcgttca ggctggcgat ttgtttagcg taattgttga tctgatcaac
    27961 gctggcacct atcgcgatat tgacctgttt gtcctggtcg cgcagatatt gatcggtggt
    28021 tttaaactga ttcaccaacc cttctgattt cccaatcagc gcctggcgcg ctgccgggtc
    28081 ttccgcgtta cttaccagcg tttgcaggtt ggtgaagaaa tcctgcatct gtgttgccag
    28141 cgaagaggta ctggtggaga gcatattgtc gattttcgac atctgctcat agcgggcagt
    28201 caggccgcta ctttgcgtct gcgccgcacg taactggttg gtaataaatg catcatactc
    28261 acgctgcaca ccagaaacgt agacgccatt gccaacccag ccgccagcgc ccagcgtgct
    28321 attggcctgc gccataatag tggtttggcg ggtatatccg gcaacgttat agctggagat
    28381 attattactt gccgtattta acgccgcctg ggccgcgttc agtccgctca tggcgttatt
    28441 gatcaagctg gacatggagg ttccttataa gccttcaatt acagagtatt atcggcagcg
    28501 acccgccgga cttgagttat tcagaacaga ttatcaatgt tcatactgta ggttttgctc
    28561 actttgtcgc ttatcgattt catctgctga atcatgttgg tgagtttgcg ggcatagtga
    28621 ggatcggtgg catagcccgc gtcctgtagg gcctgcgccc cctgctccgc actcgcggcg
    28681 gtcgtcacgg cagcgtagcg cggattacgt gttaacagcc caacgtaatc cgacaatgct
    28741 tccagatacg agctgtagac ccgaaatttc gcttttactt tcttcgcttc gccgttttca
    28801 tattcagtcg tggtgatttc agtgactggc cctttccagt tgccagaggc tttgacgcca
    28861 aacaggttat aactaggctc gccgttttcg cggcggattt gccgttgccc ccagccagat
    28921 tcaagcgccg cctgagcgag gatcaaatga tgtggcacac cgctttgctg gcttgccagt
    28981 tgggcgggca gtgagagttg cgcgaggaat gctttactgt tacccggcaa cgaatcatcg
    29041 tagttacgtg gtacggcctt ttgcaccagc tgagaaagcg cctgattttg ataacgcacc
    29101 acagtttcga gcgggaattt catcggtgct gctggcgtgg gctcctctgg caatggttgt
    29161 tctggcgtca tctgtttaac catcatctct gcaagcccca gacctttgcc cgccgtcatc
    29221 tgttgggcaa tctgctggtc atacatactg gtatacaggc gagtgtgctc gctgctgaac
    29281 aggccatctt ttggtaaagc gtcgcgcatg cttttcaaca tcatctgcac gaacatccct
    29341 tccacctggc gggccaccgg acggatattt gccgctggat cttcgcccgc tttcgccttt
    29401 agttcgttga gtgattgcgc atcccaggcc gcacttgcca gtagtttgct gtcgctgatc
    29461 atcagatgat ttccagtttt gcccgcagac atcccgcact ttgcattgat tgcaaaatag
    29521 acatcagatc catcggcgta gcgcccagcg cattgagcgc gcgcaccacg ttattgaggc
    29581 tggcgctgga acgtacgctt tgcagcgaac cgccgctctg gcgtaaatcg atctgcgttt
    29641 gtggagtaac cacagtctgt ccaccaccaa acggtgtatc tggctggctg acattggcct
    29701 gacgattgac cgtaaccgag agattcccct gcgctactgc gcagctgtcc agggtcactt
    29761 cgcgattcat caccaccgag ccagtgcgcg agttaatcac tactttagcg tcctgcgggg
    29821 tgacattaac ctgcatattc tggatatcgg caaggaaacg gacctgggaa ctgttgccac
    29881 ttggtacgcg cacctgaata gtccgcgcat ctaacgcggt ggcgctgcca tatccacgca
    29941 cgcggttgat ggtgtcagcg atttgctgcg ccatgctgaa atcttcgtcg ttaagttgca
    30001 aattaagggt attcccgacg ccaaactggc tgggcaattc acgttcaata accgcaccat
    30061 tggtgatccg tccaccgttc agttggttca cctgaacact gctaccgcca gcggaggctc
    30121 cagcgccacc aaccagaata ttgccctgcg ccagcgcata cacctgactg tcaacgccct
    30181 taagcggtgt catcaacaac gtaccgccac gcaagctttt ggcattaccc atggaagaaa
    30241 ccaccacatc gatggtttgc ccctggcgtc caaacggcgg aagtgacgct gtcaccatta
    30301 ccgcagcgac gttttttagc tgcatattgg tgcccgtcgg aacggtaatt cccagctgtg
    30361 agagcatgtt attaagcgtt tgtgtggtaa acggcgtctg ggttgtctgg tcaccggttc
    30421 catccagccc caccaccagg ccatagccaa tcagtgagtt ttgccttacc ccctgaacac
    30481 tggtgagatc gcgaatacgc tcagcctgag ccgccgtgat aaccagtaga agaattaatg
    30541 cagagagaaa tttaatcacc acagcctcac ttacattggc gacaggttaa ggaagaaacg
    30601 ctgcaaccag cccatatttt gcgcttcgtt aatgtagcca ttgcctacgt attcaatgcg
    30661 cgcatccgcc acctgagtag atggtacggt attgctgccg ctgatagtgc gtggattaac
    30721 cacgccagag aagcgaataa attcggtacc ctgattaatg gcgatctgtt tttcacccac
    30781 cacatgcagg ttgccgttga ccagtacctg gtcaaccgtc accgtcaacg taccgctaaa
    30841 ggtattgctg gcattggccc cgccttttcc gttgaacgtg ttaccaccgg aggcttcgac
    30901 atcggcacga gcgttaccaa acaacccctg caaatagcgc ggcacagtat caaagccaaa
    30961 attagtttta ccgtcacggc tggcattcgc agaggagctt ttgctggcgc tgacgttctc
    31021 ctgcaacacg atagtcagcg tatcgccaat attgcgtggt cgacgatctt caaacagcgg
    31081 ttgatagcca tagttaatcg gctgagcaga ctggaaaata gacccgttgg cgacgggcgt
    31141 cggaccggga accggttgtg cactggtcgc cccttgtacc agcggcgtgg agggtatcca
    31201 ggcgcagccg gttagtgaaa gcaccaacaa gctggaaatg gcataagtat gcgcagcgtt
    31261 tttttgcatg gctatcatct tcaaaaatca gtaaaccggt gaacctgcca ccggttaagc
    31321 cttagagttg cgtcagtttt tgcagcatct gatcggtggt ggacaccgct ttactgttga
    31381 tttcgtaagc gcgttgcacc tgaatcatat tgaccagttc ttccgccacg ttgacgttag
    31441 acgtttcaac atacccttga tacagcagtc ccgcgccgtt caggcccggc gtgctttcgt
    31501 tcggtgcacc ggaggattgc gtttcggtgt agaggttttc gccaatgctc tccagtccgg
    31561 tgtcattcat aaaggtggtg agattgagct gcccaacctg aactggagct gcctggcctt
    31621 gttgggttac gctgaccacg ccatcacgac cgatggtgat acttaacgca ttcgccggaa
    31681 tggtgatcgc tggctgcacc tgaaaaccac cggccgtcac cagctgcccg ttctgatcca
    31741 cctggaaaga gccgtcacgg gtataggctg atgagccatc tggcaacatc acctggaaaa
    31801 agccctgccc tttaatcgcg acgtctttac tgttattggt ttgcgacagg ttgccctggc
    31861 tgtgtaaacg ttccgtggcg accgggcgta cccccgtacc gatttgtaat ccggatggta
    31921 aggtggtttg ttcggaagac tgtgcccccg gctggcgaat ggtttgataa agcagatctt
    31981 caaacaccgc gcgctgacgc ttaaaaccgt tggtactaac gtttgccagg ttgttggcaa
    32041 tgacgtccat attggtttgc tgggcgtcaa ggcccgtttt ggcgatccat aatgaactga
    32101 tcataaaatg tcctgtagtg gattagtgac aaagtgcata tgtcttcttg ccggatgcgg
    32161 cgtaaacgcc ttatccggcc tacaagagct cgcaaaatcg ataaattgcg attgccccca
    32221 tagacccgat aaacttgcgc atagggcaac ttatacttgt catgtatcct ttcaattaac
    32281 tcatcgacag cagttggttg gcacggcctg cgttatcatc gacgctgctg atcaccttca
    32341 tctgcatttc aaaacgccgt gcgctggcaa tcatgtcgct cattgccgca acggcattga
    32401 cgttactgcc ttccagaacc cccgacatca cacgcaaggt tggatctgcc tgcagtaccg
    32461 gcccacgcgt agcctggctt tctgcgctta aacgaaaaat gccatcgtca ccgcgctgga
    32521 cttcgctgcc cgtggctttc accagcttca gacgccccac tggcgcaacc gtatttgccg
    32581 gatcgcccgg attcagcgcc gagattgtgc catcggcagc aatagtgatt tccgcccctt
    32641 ccggcacagc aattggcccc gcctcgccta ccaccggatg tccctgaatt gtcagttgcc
    32701 cggtgggatc aacctgaatg ctgccattac gcgtataccc ttcactgccg tcagcggtct
    32761 gcacggccag ccaaccatcc tgctgcaacg caacgtccag cgggcgcgag gtgtaatcca
    32821 tcttgccggg tgtcatgtct gcacccggtg ttgacgccgt taccagcgtg cgcgtgggca
    32881 gtgaaagccc ttccaccggc actgcgcgca aggcgtttaa ctgcgcgcga aaaccgggcg
    32941 tagaggcatt ggccagatta ctggcggtta ccgcctgttg attcagcgtc tggctggctg
    33001 ctcccatcgc ggtataaatt gcgtgatcca ttgagctatc ccgtcagcga ttagcgtaag
    33061 ttaaccagcg tattgaggat ctggtcctgg gttttgatgg tctgggcgtt agactgatag
    33121 ttacgctggg caacgatcat attgaccagt tctttactga gatcgacgtt ggacgcttcc
    33181 agcgcaccgt tggtcagtgt gccaaagttg cccgtcccgg ctgtccccaa cagcgccacg
    33241 ccagaagatt gcgtagcgga ccagacgttg tcgccttcgg atgccagacc ttcgttgttg
    33301 gcaaagttcg ccagtacaat ctgccccagc agttgggttt gttcgttgga atagttgccg
    33361 acaaccgtac cgtcatcatt gatttgataa ctcaccagat cgcccggttt gtagccgttc
    33421 tgggtggttg ccacaatgtt gttagcgccg gtattttgct gcatggagtt gaggaagctc
    33481 aggctaaacg tggcgggatc tgcgccgtta attgcgccag tggaaatatt cgctgttgga
    33541 tttgaggtca gaacgccatt ggcattaaac accagtgttg tcgctgtctt cgcaatgctg
    33601 tttggatcac tactatcctg ggtatagacc tgccagttat tatccccggt cttcacaaag
    33661 tagacgctca tgtcatgagc attaccctga ctatcgaaaa cagtcaccga accttttttg
    33721 ttatagctat ccgcattgct ggcatcaaat gtgttaacag agggaagcgg atcactggaa
    33781 ttcaggttga tctgcatcga cgccgtggtg gtagttttcg ctgccatcag ggtattcggg
    33841 atcgaaatat tggtcggatt cgccccttgc tgaatagtcg gcggcgtacc ggttgccggg
    33901 taccccgtca gctgtaaacc ttgcatattc accaggttac ggttttcatc cagcttaaat
    33961 tgtccgttac ggctgtagaa caccgaacca ttgctgtcta ccagacggaa aaaaccgttc
    34021 tggctgatag caacgtccag accacgcccg gtgttggtgg tcgtgccatc ggtaaagtcc
    34081 tgagtgatac cagcaacttt tacccccagt cccactttcg aaccggcaaa catatcggca
    34141 aaagaggccg tgcctgattt aaagccgtag gtggcggagt tggcgatatt gttgccaata
    34201 acatcgaggt tggtggcggc agcgtttaat ccgctaaccg cttgagaaaa ggccatgact
    34261 gactcctgaa atgtgaaggc ttaaattatc tgccgtactt cgtcgagggt ggtggtgccg
    34321 taagtgccga gatccagcgt attaccgttg ttgccgcgga tcacaccctg caccagagca
    34381 aactgcagcg gctgggcaac cagttgtgta ccaccgttac tggcgctaat cgctacattg
    34441 taagaaccgt tcggcgcagt gctgccgtca gtcaacgtac cgtcccaggt gaaactgtgg
    34501 actccggcag tcagttcacc gatatcaatg gtacggacaa ccgcaccgtt tttatcggtg
    34561 atggtggccg tcactttgtc tgccgcctgt tgcagctcaa caccaaacgg cgtggtcgtg
    34621 gtcacagccc cttcttcact gccggttccc gcaagaacag tggtgccggg gatcatcacg
    34681 ccgtgaccga tcaggttact ggcctgtaac gactggctgt tatcaatctg tcctgaaata
    34741 gatccgagcg tggtattgag tttttcaatc ccactgaccg tgctgatttg tgccaattgc
    34801 gacgtcagct cgttgttttc cattggattg gtcgggtcct ggtttttcag ctgcgccacc
    34861 aacaaagtca gaaaactgct ttgtaaatct gcggcgttgc tgcccgtgag cgaactactg
    34921 ctggtggtac tgacgccggt attcgtcgga tcggtggtgg ttaccgcaat ggacatagct
    34981 ttctccttta ttgaccgagc gtaagggttt tcagcatcat acttttcacc gtgttgagca
    35041 cttcaacatt ggcctgataa ctgcgtgacg ccgacatggt gttaaccatc tctccgacga
    35101 catcaacgtt tggcattttc acgtagccct ttgcatccgc cagcggatta cccggttcat
    35161 aaaccagttt gtccggggcc tgactttcta taacatcggc aacctttacg ccgcctgtcg
    35221 cagcacctgg cgcagcgtta acctggaata ccacctgttt tgcccgatat ggctgtccat
    35281 cgggaccggt cacgctatca gcattcgcca gattactggc cgccacgttc aggcgctggg
    35341 actgggcagt taacgccgac ccggcgatat caaaaatatt cagcagtgcc atccgttaat
    35401 ttccgctctg taaaacgttc atcatgcctt tgatctgccc actcaacgcg ctaaggctca
    35461 tctggtattg caggctgtta tcggcaaact gggtgcgttc gcgatccata tcgacagtat
    35521 taccgtcaag cgaaggctgg tccggaatac ggtattgcag ttctgcggaa ggaggcgtca
    35581 gcgcctgcgc cggaatgtgt ttcgttgagg tcatcgtcag agcaaccaca ctggttgcat
    35641 cccgtccacg ttgcatgact tttttaagtt cactggcaaa atcgatatcg cgcgcctgat
    35701 aaccaggggt atcggcattg gcgatgtttg ctgccagcac ttcctgacgc tgggcgcgca
    35761 gattgagcgc ctcttgttga aaacgtaagg cggcgtcgag cttatcgagc atatctcctc
    35821 cgcaggtatc aaaattctgc catcagctta aatgccttta cgcgcgcgtt atcggcggaa
    35881 taaacgcaaa atgggtcgct atttatgccg ttgatggtca ttgcggacag gtacaattca
    35941 cgttgtagaa atggctgggg gcgaaaatgc tgacaataaa acgtagcgtg gcgatcatcg
    36001 cgatactgtt cagtccgtta agtacggcga gcaatctcac atcgcaattg cacaactttt
    36061 ttagcgccca actcgcgggg gtaagtgatg aggttcgtgt ttctattcgt acagcaccca
    36121 atctgctacc gccgtgcgat cagccattgc tttcgatgag caataattcc cgtctgtggg
    36181 gcaatgtgaa tgtgttggca cgctgcggta acgacaaacg atatttacag gttaatgtgc
    36241 aggccacagg aaattatgtg gttgccgcga tgcccattgt gcgtggagga aagctggaag
    36301 ctggcaatgt caaactgaaa cgcggacggc tggatacctt gccaccgcgt acggtgctgg
    36361 atatcaatca acttgttgat gccgttagcc tgcgcgatct atcacccgat caacctatcc
    36421 agttaaccca ctttcgccag gcatggcggg taaaagcggg acaacgtgtc aacgtgatcg
    36481 ccagcggtga tgggtttagc gccaacgcag aaggtcaggc gctgaacaat gcagccgtcg
    36541 cacagaatgc gcgggtgcgc atgatatcgg gacaagtggt cagcggcgtt gttgatgcag
    36601 atgggaatat tcttataaac ctgtaagctg ttaaagatta cccgtccctt gccgataaat
    36661 aagcaacaca tgataaaagc gccctcaatg aggaataaac catgagtatt gatcgcactt
    36721 cgcctctgaa gccagtaagc accgttcaac cgcgcgaaac cactgacgcg ccggtaacaa
    36781 acacccgggc ggcaaaaaca accgcctcca ccagcaccag tgtgacgtta agcgacgcgc
    36841 aagcaaaact gatgcaaccc ggcagcagtg atatcaatct tgaacgtgtc gaagcgttaa
    36901 agctggcgat tcgtaacggt gaactaaaaa tggacaccgg caaaattgcc gatgcgctga
    36961 tcaacgaagc gcagcaagac tttcagagta actgatcgta tgacgcgtct tgcagagatc
    37021 ctcgaccaga tgtccgctgt gcttaacgat ctcaaaacgg taatggatca agagcagcaa
    37081 catctctata tggggcagat caacggcagc cagttgcaat ggattacaga acaaaaaagc
    37141 tctctgctgg cgacgctgga ttacctcgaa cagttacgca ggaaagaacc caattcagca
    37201 aatagcgttg atattagtca acgctggcag gaaattactg tgaaaacgca gcaactacgc
    37261 caaatgaatc aacataacgg ctggttactg gaaggacaga ttgaacgcaa tcaacaggcg
    37321 ctggaaatgt tgaaaccgca tcaggaaccg acgctatatg gagcgaacgg tcagacctca
    37381 acaacccatc gcggcagtaa aaagatttcg atctgaagat tatctccggc ctgcactgca
    37441 ggccggaatg cattgttaca ccgtccggcg ggcaaactct ttaactttga agcccagtac
    37501 cgccagtgca gcgaagtacg cggcaatccc cgccagcacg accgccatta aacgcagtaa
    37561 acgccagggc atggtaccca atgaccactc cggcatgata tgtaacatac ctaaaagcac
    37621 gccagacatc accagtaccg ccaccaccag acgcaacaga aacgccatcc agccgggttg
    37681 cggggtaaag attttctgct tacgcaactg ccagtaaagc agcgaagcat tcagacacgc
    37741 cgccagacca atagaaagtg acagcccggc atgtttcaac ggaccaataa acgccaggtt
    37801 catcaattgc gttaaaatca gcgtaacgat ggcaattttc actggcgttt taatgtcctg
    37861 gcgggaataa aagccaggag ccaacacttt cactacaatc aggccaatca aacccaccga
    37921 gtaggcaatt aacgcccgct gggtcatcag cgcatcaaag gcggtaaatt taccgtactg
    37981 gaacagcgaa acggttaacg gaccggaaag aatgcccaac gcaaccgcac ttggcagcgc
    38041 caacaggaaa caaagacgca gcccccagtc catcaaacgg ttgtattcat catgattgcc
    38101 actggcaaaa cttttcgaca gcgacggtag caaaatggtg ccaagcgcca cgcccagcac
    38161 accggacgga aactccatta agcggtcggc gtaatacatc cacgacaccg aaccggaagc
    38221 aagaaacgag gcaaaaatgg tgttgatgat taaggagatc tggctcacag agacgccaag
    38281 gattgccggt cccatctgtt tcaccacacg cattgctccg gcatcgtgga agttaatgcg
    38341 tggcaaaacc agcatgccga tcttcttcag atgaggtagc tgatacacca gctgcaggat
    38401 gccgccgacc gtaacagccc aggccagcgc cagcaccggt ggattaaagt acggcgcggc
    38461 aaacaacgca aaaccaatca tgctgatatt gagcaatgtc ggggcaaacg ccggaattga
    38521 gaagcggttc caggtattca gaatcgctcc caccagcgac gccagagaga tcagcaagat
    38581 atagggaaag gttatcttta gtagctggct ggtcagggca aatttgtcgg cagtgtcagc
    38641 gaagccaggc gcggtcacca tgatcaccca cggtgcagcg agcatgccag cgaccgtcac
    38701 aaccgccagc gcaagtgtca gcaggccaga aacataagag acaaagaccc gcgtggcgtc
    38761 ttcaccctgc ttgcttttat attctgccag aatcggtaca aatgcctggg aaaatgcccc
    38821 ttcggcaaag atacggcgta acaagttagg aagtttaaaa gcgacgaaaa aggcgtcggt
    38881 tgccatccct gcgccaaaga ttctggcgac aattgcgtct cgtgcgaagc caagcacacg
    38941 cgaaaacatg gtcatcgagc tgacggcggc cagcgatttt aataaattca tcggtgttct
    39001 aatccagacc cacgggcaaa acgcctgcaa ggcaggcgct gaaagaggcg aatagtctac
    39061 ctggattatg gtgaattgct accgccagat gttacagggt ttattcactc atcgcatcgc
    39121 gccagatctt gtcaacgata cgttgcgcca gcacggcctg ttcgccggcg gtttgcggaa
    39181 ctgtctgatt ttgcacgcat tcaatgaagt gccgcgcaca gccgacaaat ccacgttgct
    39241 cgagcgtact ctgccaacca ggaatcggtt tatgcactac gccctgcccg cgctcctcac
    39301 gccattcgcg catatccgta atgtcgatga gcgcaccatc agtcacggcc tgcacggttt
    39361 cacgctgact tccggcacgg cgatgcatac aggtggtaat ctgcaaagga ccagccgaaa
    39421 aatggtgctc ggcaaacagc atttcgccgg actcattggt cagcaacgta ccgctttcga
    39481 gagttgcatt gccgccagac aaccacagcg cggtatcaac cacatgcaaa taatcatcca
    39541 gcaatgtgaa ataaagatcg tgtggcccga cgctattgct acgatgttta tccattctta
    39601 gcgaggacgc ggtggcgagt tgcgttttta actcaccgta gagtggtgcg aaacgacggt
    39661 taaaaccgac catcaacgtc agttttttcc gcgccgccag ttccaccagc cgttcagcat
    39721 cgcgcagatt ttctgccagc ggtttatcga cacagacatg tacccccgca ttgagtaacg
    39781 tactgaccac atcaaagtgg ctggcggtgc tggaatgcac aaaaaccgca tcgcaactgg
    39841 cggcaaggct ggataacgaa tcagcataag gaatgcgcca gctgtcacaa attggcaagg
    39901 ctttggcgcg cgtaggcgac cacgcacctt gtaacgtcca gtcagacgct gccgccagca
    39961 ccggtaacca cgctttttgc gcgatgccgc ctaaccccac tacgccgata cgtaattttt
    40021 tcactttaat ctcccagatg ggccagcaac gaatcaagac gctgtttcag ttctgccact
    40081 tcgatttcca gggcttcgac acgggcctgt aaatcaccgt caaccgcgtt caacatatcc
    40141 gttaccgccg gcggattctc aacctcacca ctgaaaagat gcatgtagcg gctttcgcgt
    40201 ttacccggtt cgcgggccag acgcaccaca aaaggaccat cttcgcgact cgccagttgt
    40261 tccagcgtcg actccacttc cgccatatcg ctgaattcat acattcgcgc ggcgcggctg
    40321 cgcagttccc ccggcgtctg ggcaccacgc aataacaacg tggtgattaa cgccacttct
    40381 gcggcgctca gtttcagatc gccaaattct gaattacaaa aacgttgctc atatttggtg
    40441 acccgattac caaaaccgct caccgtgcgt agataatgac gtttgaccag attatccagc
    40501 tgttcctgca cttcagattc actcagattc ataaccggtt cacggttcgt tttctgatta
    40561 caggccgtga ctacaccatt tacggagagc gggtattgct ccggcgtggt cacctgtttt
    40621 tccagcaagc aaccaatcac gcgagcttcc atggcagtaa gttgatattt catgagattc
    40681 tccttagcgg ccgggcgtcc agtctggggt agttaatgcc gtcagtacgt gatcgcgcca
    40741 ttgtccatca atcaacagat agtctttcgc atagccttct ttttcaaaac ccagtcgcgc
    40801 cagtaaatca ccgctgcgtt tattgtgcgg catataatta gccataatgc gatgaatatg
    40861 ttgggtgcgc tgcatataac gaatggctgc gctcagagct tcaaacatga gtcctttgcc
    40921 ctgccatttt tgcccaatcg aataaccgag atagcaggca tgaaaagagc cgcgaacaac
    40981 attggaaaaa ttggcaacgc caataatctc tttttcgtcc gggtcgaata agccaaagta
    41041 aaaagctgaa ccttgtttat gaaattcgct aatcatccct agcctggcct gccagcctga
    41101 tggataacag tggctttcgt cgcgcactgg ctcccagggc ttgaggaaat ggcgattctc
    41161 tgcgtaataa tccgccagac gccaggcatc acgatcatgc accagacgca cgaccagtcg
    41221 gtctgtggtt aagcgcactt ttggcacgtt actgcgatag ccaaacattc tatacctact
    41281 ccttcccgta acgtctttaa tacgctgtaa gtattactat acctgcgcaa ccagaccctg
    41341 tgaaaagtcc agaatgccat tttctgcatt attcacatat ttgataaaaa ctctctatca
    41401 aagcgacgtt ttgataaaaa aatattgtcc ccgaagtgat gggaaaagtc ttagcggcaa
    41461 tgcagaatag aaagtgctca tcttttattt cccgggaggg gaaatgtccc gcgtgtcgca
    41521 ggcgaggaac ctgggtaaat atttcctgct catcgataat atgctggtcg tgctggggtt
    41581 ctttgttgtc ttcccgctga tctctatccg cttcgttgat caaatgggct gggccgccgt
    41641 catggtcggt attgccctcg gtctacgcca gtttattcag caaggtctgg gtattttcgg
    41701 cggcgcaatt gccgaccgct ttggtgccaa accgatgatt gtcaccggta tgctgatgcg
    41761 tgccgccgga ttcgccacaa tgggtatcgc ccacgaaccg tggctactgt ggttttcatg
    41821 cctgctttcg ggactcggcg gcacgttgtt tgatcctccg cgttcggcgc tggtggtgaa
    41881 attaatccgt ccacagcagc gtggtcgttt tttctcgctg ttgatgatgc aggacagtgc
    41941 cggtgcggtc attggcgcat tgttggggag ctggctgttg caatacgact ttcgcctggt
    42001 ctgcgccaca ggggcagttt tgtttgtgct gtgtgcagcg ttcaatgcgt ggttgttacc
    42061 ggcatggaaa ctctccaccg tacgcacgcc cgttcgcgaa ggcatgaccc gcgtgatgcg
    42121 cgacaagcgt tttgtcacct atgtcctgac gctggcgggt tactacatgc tggctgtaca
    42181 agtgatgctg atgctgccaa ttatggtcaa cgacgtggcg ggcgcgccct ctgccgttaa
    42241 atggatgtat gccattgaag cgtgtctgtc gttaacgttg ctctacccta tcgcccgctg
    42301 gagtgaaaag cattttcgtc tggaacaccg gttaatggct gggctgttga taatgtcatt
    42361 aagcatgatg ccagtaggca tggtcagcgg cctgcaacaa cttttcaccc tgatttgtct
    42421 gttttatatc gggtcgatca ttgccgagcc tgcgcgtgaa accttaagtg cttcgctggc
    42481 agacgcaaga gctcgcggca gctatatggg gtttagccgt ctgggtctgg cgattggcgg
    42541 cgctattggt tatatcggtg gcggctggct gtttgacctg ggcaaatcgg cgcaccagcc
    42601 agagcttccg tggatgatgc tgggcattat tggcatcttc actttccttg cgctgggttg
    42661 gcagtttagc cagaaacgcg ccgcgcgtcg tttgcttgaa cgcgacgcct gatttgatac
    42721 tcccccctga ctcctccata ctgaaagtga actgacagcc aaatggagga gtcacgtgaa
    42781 gctatacatt tacgatcact gcccttactg cctcaaagcc cgcatgattt tcggcctgaa
    42841 aaatatcccc gtcgaattac atgttctgct caacgacgac gcagaaacac ccacccggat
    42901 ggtcggtcaa aaacaggttc ccattctgca aaaagatgac agccgctata tgccagaaag
    42961 catggacatc gttcactatg tcgataaact cgacggcaaa ccgttactga ccggcaaacg
    43021 ttcccctgcc attgaagagt ggctgcgcaa ggtcaatggc tacgccaaca aactgctgtt
    43081 gccgcgtttt gccaaatcgg catttgatga gttttctact cctgccgcgc gcaaatattt
    43141 cgtcgacaag aaagaggcca gcgcgggtaa ttttgccgac ctgctggccc actctgacgg
    43201 tctgattaag aatatcagcg atgatttacg tgcgctggac aaactgatcg tcaaaccgaa
    43261 cgccgtgaat ggcgaacttt cggaagatga tattcagcta tttccgctcc tgcgtaatct
    43321 gacgctagta gccggaatca actggccaag ccgcgttgct gattaccgcg ataatatggc
    43381 gaaacagaca caaatcaatt tgttatcatc aatggcgatt taatcctgac ccgccgcggc
    43441 agcctgtatt cgcgggttgc cgccgttttc agttttttct gctggcgcgt ttatcgctgg
    43501 tgacacatgc tgcgttgtgt caggatattg acgcagattt catgtcgagg actaccatga
    43561 acaagttttt atttgccgct gcattgatcg tcagtggcct gctcgttggc tgtaatcaac
    43621 tcacccaata caccatcacc gaacaagaaa ttaaccagtc gcttgcgaaa cataataatt
    43681 tctcaaaaga tatcggttta cccggcgtgg ctgacgccca tattgttctg acaaacctga
    43741 ccagccaaat tggtcgcgaa gagccgaata aggttaccct aaccggagac gccaatctgg
    43801 acatgaactc cctgttcggt agccagaaag cgaccatgaa actgaagctg aaagcgctgc
    43861 ccgtgtttga taaagaaaaa ggcgcgatct tcctgaaaga gatggaagtg gtcgatgcga
    43921 cggtacaacc ggaaaaaatg caaacggtga tgcaaacgtt gcttccctat ttaaaccagg
    43981 cattacgcaa ttactttaac cagcaacctg cttacgtcct gcgcgaagat ggcagccagg
    44041 gcgaagcaat ggcgaagaaa ctggcgaaag gcattgaagt gaagccaggc gaaattgtca
    44101 ttccgtttac tgattaatca cgagggcgca ttcgcgccct ttatttttcg tgcaaaggaa
    44161 aacgtttccg cttatccttt gtgtccggca aaaacatccc ttcagccgga gcatagagat
    44221 taatgactgc accatcccag gtattaaaga tccgccgccc agacgactgg caccttcacc
    44281 tccgcgatgg cgacatgtta aaaactgtcg tgccgtatac cagcgaaatt tatggacggg
    44341 ctatcgtaat gcccaatctg gctccgcccg tgaccaccgt tgaggctgcc gtggcgtatc
    44401 gccagcgcat tcttgacgcc gtacctgccg ggcacgattt caccccgctg atgacctgtt
    44461 atttaacaga ttcgctggat cctaatgagc tggagcgcgg atttaacgaa ggcgtgttca
    44521 ccgctgcaaa actttatccg gcaaacgcaa ccactaactc cagccacggc gttacgtcag
    44581 ttgacgcaat catgccggta cttgagcgca tggaaaaaat cggtatgccg ctactggtac
    44641 atggtgaagt aacaaatgca gatatcgaca tttttgatcg tgaagcgcgc tttatagaaa
    44701 gcgtgatgga accgctgcgc cagcgcctga ctgcactgaa agtcgttttt gagcacatca
    44761 ccaccaaaga cgctgccgac tatgtccgtg acggaaatga acggctggct gccaccatca
    44821 ctccgcagca tctgatgttt aaccgcaacc atatgctggt tggtggcgtg cgtccgcacc
    44881 tgtattgtct acccatcctc aaacgcaata tccaccaaca ggcattgcgt gaactggtcg
    44941 ccagcggttt taatcgcgta ttcctcggaa cggattctgc gccacatgca cgtcatcgca
    45001 aagagagcag ctgcggctgc gcgggctgct tcaacgcccc aaccgcgctg ggcagttacg
    45061 ctaccgtctt tgaagagatg aatgctctgc agcactttga agcattctgt tctgtaaacg
    45121 gcccgcagtt ctatggcttg ccggtcaacg acacattcat cgaactggta cgtgaagagc
    45181 aacaggttgc tgaaagcatc gcactgactg atgacacgct ggtgccattc ctcgccggag
    45241 aaacggtacg ctggtccgtt aaacaataaa aatcgtagct tcctgttgtc attaggtcat
    45301 tctacctgta taaataacca gtatattcaa cagggggcta ttatgcgaat tgaagtcacc
    45361 atagcgaaaa cttctccatt gccagctggg gctattgacg ccctggctgg cgaactttcc
    45421 cgccgtattc agtatgcgtt tcctgataat gaaggccacg tatcggtacg ttatgccgca
    45481 gcgaataatt tatcggttat tggcgcgaca aaagaagata aacagcgcat tagcgaaatt
    45541 ctccaggaaa cgtgggaaag cgccgatgac tggtttgtca gcgaataata tgcagtgatt
    45601 tttgtttttg ccgggtcgcc ccggcttttt tatttta
//
LOCUS       DAZFJL010000020        41324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFJL010000020 DAZFJL010000000
VERSION     DAZFJL010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.020"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..82
                     /locus_tag="ACHJ8C_001127"
     CDS             <1..82
                     /locus_tag="ACHJ8C_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5551021.1"
                     /translation="NFAGDCETAFALAVKFALAQLLQLLL"
     gene            complement(127..2172)
                     /gene="dcp"
                     /locus_tag="ACHJ8C_001128"
     CDS             complement(127..2172)
                     /gene="dcp"
                     /locus_tag="ACHJ8C_001128"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO5551022.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGIQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLEQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGRRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            2309..3055
                     /gene="ydfG"
                     /locus_tag="ACHJ8C_001129"
     CDS             2309..3055
                     /gene="ydfG"
                     /locus_tag="ACHJ8C_001129"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO5551023.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3144..3830
                     /gene="rspR"
                     /locus_tag="ACHJ8C_001130"
                     /gene_synonym="ydfH"
     CDS             3144..3830
                     /gene="rspR"
                     /locus_tag="ACHJ8C_001130"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO5551024.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMMQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            4007..4210
                     /gene="ydfZ"
                     /locus_tag="ACHJ8C_001131"
     CDS             4007..4210
                     /gene="ydfZ"
                     /locus_tag="ACHJ8C_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO5551025.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4246..5706)
                     /gene="ydfI"
                     /locus_tag="ACHJ8C_001132"
     CDS             complement(4246..5706)
                     /gene="ydfI"
                     /locus_tag="ACHJ8C_001132"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO5551026.1"
                     /translation="MGNHLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMCDVVTSCAQAIDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRTLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYTLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGTARVQSLLAIKAIF
                     GDDLPGNSLFTTKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(5795..7162)
                     /gene="ydfJ"
                     /locus_tag="ACHJ8C_001133"
     CDS             complement(5795..7162)
                     /gene="ydfJ"
                     /locus_tag="ACHJ8C_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFO5551027.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPHVVRAAQ
                     PSRSL"
     gene            complement(7220..8239)
                     /gene="rspB"
                     /locus_tag="ACHJ8C_001134"
     CDS             complement(7220..8239)
                     /gene="rspB"
                     /locus_tag="ACHJ8C_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO5551028.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGDGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GESFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFELDQKHCCKVL
                     LTFSE"
     gene            complement(8251..9465)
                     /gene="rspA"
                     /locus_tag="ACHJ8C_001135"
     CDS             complement(8251..9465)
                     /gene="rspA"
                     /locus_tag="ACHJ8C_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO5551029.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFDEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGEKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(9671..9997)
                     /gene="ynfA"
                     /locus_tag="ACHJ8C_001136"
     CDS             complement(9671..9997)
                     /gene="ynfA"
                     /locus_tag="ACHJ8C_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO5551030.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALIWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            10132..10473
                     /gene="ynfB"
                     /locus_tag="ACHJ8C_001137"
     CDS             10132..10473
                     /gene="ynfB"
                     /locus_tag="ACHJ8C_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO5551031.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QRAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVIIP"
     gene            10508..11068
                     /gene="speG"
                     /locus_tag="ACHJ8C_001138"
     CDS             10508..11068
                     /gene="speG"
                     /locus_tag="ACHJ8C_001138"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO5551032.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(11071..11781)
                     /gene="ynfC"
                     /locus_tag="ACHJ8C_001139"
     CDS             complement(11071..11781)
                     /gene="ynfC"
                     /locus_tag="ACHJ8C_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO5551033.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDNHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            11889..12194
                     /gene="ynfD"
                     /locus_tag="ACHJ8C_001140"
     CDS             11889..12194
                     /gene="ynfD"
                     /locus_tag="ACHJ8C_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO5551034.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDNAPAEPQ"
     gene            12103..12408
                     /locus_tag="ACHJ8C_001141"
                     /pseudo
     CDS             12103..12408
                     /locus_tag="ACHJ8C_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245348.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
     gene            12451..13065
                     /gene="dmsD"
                     /locus_tag="ACHJ8C_001142"
     CDS             12451..13065
                     /gene="dmsD"
                     /locus_tag="ACHJ8C_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO5551035.1"
                     /translation="MTHFSQQDNFSIAARVLGALFYYAPESAEAAPLVAVLTRDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            13225..14517
                     /gene="clcB"
                     /locus_tag="ACHJ8C_001143"
     CDS             13225..14517
                     /gene="clcB"
                     /locus_tag="ACHJ8C_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO5551036.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATIVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRLTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVNNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWLPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(14470..15165)
                     /gene="bioD"
                     /locus_tag="ACHJ8C_001144"
     CDS             complement(14470..15165)
                     /gene="bioD"
                     /locus_tag="ACHJ8C_001144"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO5551037.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(15290..16510)
                     /gene="mlc"
                     /locus_tag="ACHJ8C_001145"
                     /gene_synonym="dgsA"
     CDS             complement(15290..16510)
                     /gene="mlc"
                     /locus_tag="ACHJ8C_001145"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO5551038.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLERIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(16645..17538)
                     /gene="ynfL"
                     /locus_tag="ACHJ8C_001146"
     CDS             complement(16645..17538)
                     /gene="ynfL"
                     /locus_tag="ACHJ8C_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5551039.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTEAGKQFLVDSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            17645..18898
                     /gene="ynfM"
                     /locus_tag="ACHJ8C_001147"
     CDS             17645..18898
                     /gene="ynfM"
                     /locus_tag="ACHJ8C_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5551040.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMSTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHHRLHA"
     gene            complement(18948..19067)
                     /locus_tag="ACHJ8C_001148"
     CDS             complement(18948..19067)
                     /locus_tag="ACHJ8C_001148"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO5551041.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            19323..19631
                     /gene="asr"
                     /locus_tag="ACHJ8C_001149"
     CDS             19323..19631
                     /gene="asr"
                     /locus_tag="ACHJ8C_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO5551042.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            19724..19807
                     /locus_tag="ACHJ8C_001150"
     CDS             19724..19807
                     /locus_tag="ACHJ8C_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5551043.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            19907..20728
                     /gene="ydgD"
                     /locus_tag="ACHJ8C_001151"
     CDS             19907..20728
                     /gene="ydgD"
                     /locus_tag="ACHJ8C_001151"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO5551044.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(20767..21096)
                     /gene="mdtI"
                     /locus_tag="ACHJ8C_001152"
     CDS             complement(20767..21096)
                     /gene="mdtI"
                     /locus_tag="ACHJ8C_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO5551045.1"
                     /translation="MAQFEWVHAAWLALAIVLEIIANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(21083..21448)
                     /gene="mdtJ"
                     /locus_tag="ACHJ8C_001153"
     CDS             complement(21083..21448)
                     /gene="mdtJ"
                     /locus_tag="ACHJ8C_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO5551046.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            21859..22893
                     /gene="tqsA"
                     /locus_tag="ACHJ8C_001154"
     CDS             21859..22893
                     /gene="tqsA"
                     /locus_tag="ACHJ8C_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFO5551047.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(22918..24306)
                     /gene="pntB"
                     /locus_tag="ACHJ8C_001155"
     CDS             complement(22918..24306)
                     /gene="pntB"
                     /locus_tag="ACHJ8C_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO5551048.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(24317..25849)
                     /gene="pntA"
                     /locus_tag="ACHJ8C_001156"
     CDS             complement(24317..25849)
                     /gene="pntA"
                     /locus_tag="ACHJ8C_001156"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO5551049.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            26372..27316
                     /gene="ydgH"
                     /locus_tag="ACHJ8C_001157"
     CDS             26372..27316
                     /gene="ydgH"
                     /locus_tag="ACHJ8C_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO5551050.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVITG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            27502..28884
                     /gene="ydgI"
                     /locus_tag="ACHJ8C_001158"
     CDS             27502..28884
                     /gene="ydgI"
                     /locus_tag="ACHJ8C_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5551051.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAIMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            28921..29643
                     /gene="folM"
                     /locus_tag="ACHJ8C_001159"
     CDS             28921..29643
                     /gene="folM"
                     /locus_tag="ACHJ8C_001159"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO5551052.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLMNAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSLPLDGGRHLR"
     gene            complement(29640..29975)
                     /gene="ydgC"
                     /locus_tag="ACHJ8C_001160"
     CDS             complement(29640..29975)
                     /gene="ydgC"
                     /locus_tag="ACHJ8C_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO5551053.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            30104..30823
                     /gene="rstA"
                     /locus_tag="ACHJ8C_001161"
     CDS             30104..30823
                     /gene="rstA"
                     /locus_tag="ACHJ8C_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO5551054.1"
                     /translation="MNTIVFVEDDAEVGALIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVSLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            30827..32128
                     /gene="rstB"
                     /locus_tag="ACHJ8C_001162"
     CDS             30827..32128
                     /gene="rstB"
                     /locus_tag="ACHJ8C_001162"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO5551055.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLSERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            32204..33133
                     /gene="tus"
                     /locus_tag="ACHJ8C_001163"
     CDS             32204..33133
                     /gene="tus"
                     /locus_tag="ACHJ8C_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO5551056.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAELEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNFSQAALVSHIQHINKLKTTFEHIVTVESELPSAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(33130..34533)
                     /gene="fumC"
                     /locus_tag="ACHJ8C_001164"
     CDS             complement(33130..34533)
                     /gene="fumC"
                     /locus_tag="ACHJ8C_001164"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO5551057.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DRWVRPEQMVGSMKAGG"
     gene            complement(34676..36322)
                     /gene="fumB"
                     /locus_tag="ACHJ8C_001165"
     CDS             complement(34676..36322)
                     /gene="fumB"
                     /locus_tag="ACHJ8C_001165"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO5551058.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            36521..37696
                     /gene="manA"
                     /locus_tag="ACHJ8C_001166"
     CDS             36521..37696
                     /gene="manA"
                     /locus_tag="ACHJ8C_001166"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO5551059.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKATLLGDAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISECYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            37797..39305
                     /gene="ydgA"
                     /locus_tag="ACHJ8C_001167"
     CDS             37797..39305
                     /gene="ydgA"
                     /locus_tag="ACHJ8C_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO5551060.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTVNSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            39492..39950
                     /gene="tnpA"
                     /locus_tag="ACHJ8C_001168"
     CDS             39492..39950
                     /gene="tnpA"
                     /locus_tag="ACHJ8C_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
                     /protein_id="HFO5551061.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGCILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMPYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
     gene            complement(40061..>41324)
                     /gene="uidC"
                     /locus_tag="ACHJ8C_001169"
     CDS             complement(40061..>41324)
                     /gene="uidC"
                     /locus_tag="ACHJ8C_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO5551062.1"
                     /translation="RKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRAD
                     KPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGDK
                     SFGFTLGAVKIKPSESSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALGY
                     WALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDTS
                     RYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETAL
                     VSGQLTWIAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVNY
                     RLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKGR
                     EKRDGSALGDRLNYWDVKMSIQYDFMLK"
BASE COUNT        10339 a        10614 c         9919 g        10452 t
ORIGIN      
        1 taattttgct ggcgactgcg aaacggcatt cgcattagcg gtgaagtttg ccctcgccca
       61 gttgctgcaa ttactgctat aacgaagctg gtctggcgat aaatccaggc cagccattgg
      121 tggtgtttat acgttcaagc cacgatgttg cagcatcggc ataatctgag gtgccttacc
      181 gcgccattgt cgatacaggc gttccagatc ttcgctgtta cctctggaaa gaatcgcctc
      241 gcgaaaacgc cgcccatttt cacgcgttaa tccgccctgc tcaacaaacc actggtaacc
      301 atcatcggcc aacatttgcg tccacagata agcgtaataa cctgcggcat atccgccacc
      361 aaaaatatgg gcgaaataac tgctgcgata gcgtggcggt atagcaggaa gatccatatt
      421 ttccgccacc agcgcccgca attcgaaatc atcgacgtcc tgcattgctt cgttttcttc
      481 cagacaatgc cagcgcatat cgagaagtgc ggcgctaagc agttcgctca tctcataccc
      541 tttattgaac agactggcat tacgcatttt ctgttgaagt tcgtcaggca ttgcggcccc
      601 gctctgataa tgccgggcgt agcgagcaaa tacctgcgga tgtgttgccc agtgttcgtt
      661 gatttgcgac ggaaattcga caaaatcacg cggcgtgttg gtgccggaaa gcgtggcata
      721 acgctggcgg gcaaaaaggc cgtgcagcgt atgaccaaat tcatggaata aggttattac
      781 atcatcccag agtaacaacg caggctcacc ggcagcgggt ttctgataat tgcagacgtt
      841 ataaattacc gggtgcgttt cattcagcgt tgattgctca acaaaattgc ccatccatgc
      901 gccgccgctt tttgaatcac gggcgaagaa atcaccgtaa aataacgcca gccccacgcc
      961 attatgatca aaaatttccc acacacgaac gtcaggatgg tagacaggaa tatcaaaacg
     1021 ttcgacaaac ttaataccga agagctgatt cgcagtccag aatacgcctt cattcaacac
     1081 cgtgtttaat tcgaaataag gcttgagctg cgcttcatcg agatcgaatt tctcacgtct
     1141 tacctgctcg gcataaaatg cccagtccca cggctgcgcg ctaaacccgc cctgctgctt
     1201 atcgataacc gcctgtatgg aggctaattc atcgctcgca cgttgacgcg ccgctggaac
     1261 aatttcccgc ataaagttga gtgctgcttc tggcgttttt gccatctgat cggcgatttt
     1321 ccatgcggca taatgaggga aaccaagcag tttcgcctgc tgcgcgcgga tctccaccag
     1381 acgttgaatg atagcgcggg tatcattggc atcatttttt tccgctcgcg tccagcccgc
     1441 aataaacagt ttttcacgcg tcgcacgatc gcgcatttcg gcaagcgccg gttgttgggt
     1501 ggtattcagc agcggaatca gccatttgtt atccagacct ttctcgcgag ccgcctctgc
     1561 cgccagcgca atctcttgct cactcattcc tgccagctgc gcgatatcgt tcacaaccag
     1621 accgccggat ttatttgctg ccagtaaccg ctggttaaac tggctggtca gggtcgcagc
     1681 ttctgtattc agtactttta attttgcttt atcagcttgc tcaagtttgg ctccggcaag
     1741 gacaaaacgt tgatgaatca cctccaccag gcggatggat tcactatcaa gccccaggga
     1801 ttcacggcgt tgccagacag catctacccg cgcgaataat tccccgttca gatagatatc
     1861 attcgcaagt tccgccagtt cagcggaaaa ctgttcatca agacgctgta attcatcatt
     1921 ggtatgcgcc gcagtcatcg caaaaaagac gctggtaacg cgggtaagta attctccgct
     1981 ttgttccagt gccagaatag tattgttgaa atcaggtgtt tgcgggttaa gcgcgatggc
     2041 agcaatttct gcccgctttt gctgtattcc ctcatcgaat gccgggcgat agtgatgatt
     2101 ggcaatttga tcaaaatggg gagccagata cggcagtgtg ctttgcacaa ggaaaggatt
     2161 cattgttgtc attttcttct cctgaacgcg aggtgttcca tagcgtaggc ttactgataa
     2221 cggagtgcaa tcttgcaaac cagtattacc cgctcttaag catcacgtgc tatgttattg
     2281 acacacaaaa gcgttgagga acagtgagat gatcgtttta gtaactggag caacggcagg
     2341 ttttggtgaa tgcattactc gtcgttttat tcaacaaggg cataaagtta tcgccactgg
     2401 ccgtcgccag gagcggttac aggagttaaa agacgaactg ggagataatc tgtatatcgc
     2461 ccaactggac gttcgcaacc gcgccgctat tgaagagatg ctggcatcgc tccctgccga
     2521 gtggagcaat attgatatcc tggtaaacaa tgccggcctg gcattgggca tggagcctgc
     2581 gcataaagcc agcattgaag actgggaaac gatgattgat accaacaaca aaggcctggt
     2641 atatatgacg cgcgccgtct taccgggtat ggttgaacgt aatcatggtc atattattaa
     2701 cattggctca acggctggta gctggccgta tgccggtggt aacgtttacg gtgcgacgaa
     2761 agcgtttgtt cgtcaattta gcctgaatct gcgtacggac ctgcatggta cggcggtgcg
     2821 cgtcaccgac atcgaaccgg gtctggtggg tggcaccgag ttttccaatg tccgctttaa
     2881 aggcgatgac ggtaaagcgg aaaaaaccta tcaaaatacc gttgcattga cgccagaaga
     2941 tgtcagcgaa gccgtctggt gggtgtcaac gctgcctgct cacgtcaata tcaatacact
     3001 ggaaatgatg ccggttaccc aaagctatgc cggactgaat gtccaccgtc agtaattttt
     3061 atacccggcg taactgccgg gttattgctt gtcacaaaaa agtggtagac tcatgcagtt
     3121 aactcactca caagcaagaa cgaatgaccg tcgaaacgca acttaatccc acacagcctg
     3181 tcaatcagca gatttatcgt attcttcgcc gcgacattgt ccattgcctg attgcaccag
     3241 gcacaccgtt gtcggaaaaa gaagtttctg ttcgtttcaa tgtgtcacgc cagccggttc
     3301 gtgaagcctt tattaaactg gcggaaaacg gtctgattca aattcgtccg cagcgtggca
     3361 gctacgtcaa caaaatttcc atggcccagg tgcgcaacgg cagttttatc cgtcaggcca
     3421 ttgagtgcgc ggtggcgcgt cgggcggcga gcatgattac cgaaagccag tgctaccaac
     3481 tggaacaaaa tcttcaccag caacgcattg ccattgagcg caagcaactg gatgattttt
     3541 ttgaacttga tgacaacttc catcaactcc tgacgcagat tgccgactgt caactggcgt
     3601 gggataccat tgagaacctg aaagcgaccg ttgatcgcgt gcgctatatg agtttcgacc
     3661 acgtttctcc accagaaatg ctgttacgcc agcatcttga tattttctct gccctgcaaa
     3721 aacgtgatgg cgatgcggta gaacgtgcaa tgatgcaaca tttgcaggaa atcagcgaat
     3781 ctgtgcgcca gatccgccag gaaaacagcg actggtttag cgaagagtaa ttcatttcct
     3841 ctcatcccat ccggggtgag agtcttttcc cccgacttat ggctcatgca tgcatcaaaa
     3901 aagatgtgag cttgatcaaa aacaaaaaat atttcactcg acaggagtat ttatattgcg
     3961 cccgttacgt gggcttcgac tgtaaatcag aaaggagaaa acacctatga cgacctacga
     4021 tcgtaaccgt aacgcaatca ccactggcag ccgtgttatg gttagcggca ccggtcacac
     4081 tggcaagatc ctgtcgattg atactgaagg tctgaccgct gagcaaatcc gccgcggaaa
     4141 aaccgtagtt gttgaaggtt gtgaagagaa actggcacca ctggacctga ttcgtctcgg
     4201 catgaactaa gcgtgtgaat gccgccgatg gcggcattgc tttttttact tcacggaata
     4261 tttcgccacg gtcgctttcg cgccatgcgc taataaagac aagtacgctt ccgttacttt
     4321 tgtcgtaaac aaactattgc ctggtaaatc atcaccaaag atcgccttaa ttgccagcag
     4381 tgactggacg cgcgctgtcc cttcagcact actttgtact gccttctgaa taacaggtaa
     4441 cagtggatca ctgatttcta tcggatttcc ctgttcatca acaccaccga cataacgcat
     4501 ccaacccgcg acgcccagcg ccagcagatc gaacttgctg tcatgcgcca gatgccagcg
     4561 aacagaatcc aacatccgct gtggcaattt ctggctgcca tccatcgcaa tctgccaggt
     4621 tcgatgacgt aacgccgggt tgctatagcg ttcaattaat cggttagcgt aatcttgcaa
     4681 atcaacgccc tgcactttca gcgtcggcgc ttgttcctgc aacattaagg tatacgccgc
     4741 atgacgataa tgttcatcct ccatgcagtc attaatgtgc tgatatccgg caagatagcc
     4801 cagatacgcc aggaatgaat gactgccatt gagcgtgcgc aacttcatct cttcataagg
     4861 cagcacatca ctaaccagtt cggctcccgc tttttcccat tccggacgtc cggcaacaaa
     4921 gttatcttct attacccact ggcggaaagg ttcacaggca acgccagcag gatcgcgcac
     4981 accggtaagt tgttcgattt tcgccagcgt atcctctgtc actgcgggca caatacggtc
     5041 caccattgtt gatgggaaag tcacgttatc ttcgatccat tgtgccagtt ttacatcaat
     5101 ggcttgcgca caggaagtga caacgtcaca cataacatga ccgttttctg gcatgttgtc
     5161 acatgacatg acggtaaatg cgggaagtcc tgccgcttta cggcgagcca gcgcctcaac
     5221 aatcacccct gttgcagttt tcggctggtg gggattttgc acgtcggcaa cgaccatcgg
     5281 gtgatcgagc attaactgtc cggtcgccgg agagtggaaa tacccttttt cggtgattgt
     5341 cagagagaca atcgcgattt gcggttcaca catcgcagcc aacacggttt ctaagccatc
     5401 tatttgtacg tgtaaggctt ttttaacgac gccaacgacg cgagccgtcc acgcatcagc
     5461 cgacatttcc gcaacggtat aaagattatc ttgctgttgt aaatcggcaa tttgctgttc
     5521 gccgccgatt aagttgacct cataatatcc ccagtcactg aaatgttccg tagcaagaat
     5581 atcggcatac acgccctgat gcgcacggtg aaatgcacca aagcctaaat gaacaattct
     5641 tggggccagg ttattacgat cataaacagg gagcgtcgct tttgctgata acaaatgatt
     5701 tcccataaca attccttaaa attaaatata gcaagctaca tgttttgtta tatgaataaa
     5761 aatcccctct ccggtaagag aagggattga ggatttacag acttctggac ggttgcgcag
     5821 ctcttacaac atgtggttga tcttctgcgg cgtcttccag cgcacttaaa tcacggtctt
     5881 tcacttctgg cattttcagc gcagagatta aaccaatcac agaatatgcc atgatcataa
     5941 tggcgatcgg ataccaggat tccgtcatgg tgcagaaaat acccgccagg ataggaccaa
     6001 aaccggaagc gataagacca ccaatttctt tagaaatagc catccgggta aagcggtttt
     6061 tacagccgaa catttctgcc atggtaatgt tttccagagc aaataatccc agcaccgcac
     6121 agttatgaat cacaatcagt gcaaccataa tggtgctcgg ggcatagctt ttatctacga
     6181 tgatagaaag cattggccac gccagcacaa tcgcagaggt attcataata atatacggga
     6241 tccggcgacc tattttatcg gataaccaac caaggaacgg aatggtcata aagccgagaa
     6301 tcgaactgat catcaatgca tctgttggaa ttgctttgtt aaacaataac gtctgcacta
     6361 aatagcctgc aaggaaagtc tgaattaaac ctgagttacc agcctgacca aaacgcagcc
     6421 ctgttgccag ccagaaggat ttgctctgga acatgctacc agcaggtgca ggttttgctg
     6481 tcggttggtt gctgtcgtta accttctcaa agaccgggct ttctttcaga ttcatacgca
     6541 accagatagc aaagaccatc acgacaacgc tcgccaggaa aggtatacgc catccccacg
     6601 ccagcagttc ctctttactg agaatgaaga acataaaggc ccagattgcc gttgcgctca
     6661 aggttccgca gttagttccc atagccacaa atgaggagat aattccgcgc ttaccttttg
     6721 gcgcatattc cgccagcatc gtaccggcac cggaaatttc cgcacctgca cccaacccct
     6781 gaataatacg cagcgtcacc agcaagatgg gggcaaaaac accaatttgt gcataagtcg
     6841 gtaacacacc aattaaggtg gtacagatcc ccatcatggt gatggtaata aagagcactt
     6901 ttttacgccc tattctgtcg cccattttgc cgaaaataaa tgctccgaca atacgtgcca
     6961 cataacctgc accataggtt cccattgcca gaattaacgc cattgccgtt gatgattcag
     7021 gaaaaaatat ttcatgaaac actaacgctg cgccgagcga atataactgg aagtccataa
     7081 attcaagcgc agtgcccaac catcccgata ctgctgcttt caccaaatcc ttagtacttc
     7141 tttcgtgttt ttctattgtc ataatggtta tctctaaaaa agaggtaaga tgcgtactac
     7201 ttactcgccg ttattggtat tattcagaaa aagtgagtaa gactttgcag caatgttttt
     7261 gatccagttc aaataaacta atggcatccg caacatgctg gaaatcaaat gtatgggtaa
     7321 ttagtttttc tggtttaatt aaccctttac ttaaccagtc gataacgacc gggaatttat
     7381 ttgcatttaa gcgtgaagag aaaatagaga gttcttttcc ggtaattcct tgctgaatca
     7441 cttcagacgg ttcactggag aagcccatca atacaatacg tgccgctgga gaagccagcg
     7501 ttacggcctc tttcaagatt gaaggatgac aagcggcatc gataattaaa gtcggcttga
     7561 tgcctttttc agcgaaactc tcgccaagcg gtgtctggct gttattaatc gcccagtctg
     7621 ccccgctctc tttcgctttt tccagtcgtt catcaatgcg atcggcaaca atcacatttt
     7681 taacgttata gacgcctttt aatacctgaa cgatcgtcag gccgattggg ccggcaccgt
     7741 aaaccagaac ggtatcattt tcagtcggtt gaccatgacc ggtaacgtta gccgcaatgg
     7801 taaaaggttc gatcatcacc gcatattgat cggccactgc ttcaggaatt ttccacgcat
     7861 tttttgccgg aaccacggca tattcactga aaccaccgtc agcgtgcaca cctaatacag
     7921 caagtgtcgt acaaacgttc ggcttaccta tagagcacgg atagcaatgc ccacagctga
     7981 ccaccggatc gacagcaacg cgttcaccga ctctggcgct ttccacgccg tcacccactg
     8041 catcaatgac gccaaagaat tcatgaccaa tgacgcgcgg atatttcgca aaaggattat
     8101 gcccacggta aatatggcta tctgaaccac aaattccggc aagtttaact tttactcgta
     8161 cttcacccgc tgacggggtg ggtatttcac gttcgataat cgacagttga ttcggttttt
     8221 caattaaaat gcttttcatt accttactcc ttaccagttc cacagcgtgc catcttccag
     8281 acgtgcgact ggcagataag caggttcata gggatatttc gccgccagct tttcatcgaa
     8341 ttcgatgcca agacccggtt tttctcccgg atgcatatag ccgttatcga aagtccagtt
     8401 gtgcgggaag acttcgagca tttgttcgga ataacccatg tattcctgga caccgaaatt
     8461 ggggacccac agatcaaagt gcagcgccgc agccatgcag actggtgaca aatcggaagg
     8521 accgtgtgag ccagtacgta cctgatacag cgaagcaaaa tcggcaatcc gacgcatacc
     8581 ggtaattccg cctgcatggg tcagcgtggt gcggatataa tcgatgagtt gctcttcaat
     8641 cagttgtttg cagtcccaaa tgctgttgaa gacttcaccc accgcgatgg gtgtgacggt
     8701 atgttggcga atgagacgga agcattcctg gttttccgca ggcgtcgggt cttccatcca
     8761 gaacatgcga taatcttcaa tgctcttacc aaagcgcgcc gcttcaatag gcgttaagcg
     8821 atggtgcatg tcatgcagca gatgttcatc aaaaccaaac ttgttacgta ccgcgtcaaa
     8881 caatttcggc atgaaatcga ggtatttctc cgtcgaccac agctgctctt ccggccactg
     8941 tcctttggtt gcgggttcat aagccagacc tttacctttc gacatgccgt aggtggtttt
     9001 cataccaggg attccgcact gcacgcggat ggctttgaat cccagctcct gatgacgggc
     9061 ataatcatcc agagcttcat caatactgtg accggtggta tggcaataaa ccatcacccc
     9121 ttcacgagac gcgccgccga gtaactggta aagcggcatg ttggcagctt tggctttaat
     9181 atcccacagc gccatatcaa ccgctgaaat ggccgacatc gtaaccggac cgcgacgcca
     9241 gtaagcacct ttatagaaaa actgccagat atcttcgata cggtgtgcat cgcgaccaat
     9301 aagctgcgga caaaggtgat cctgcaaata agaggccacg gaaagctcac gtccatttag
     9361 ggtggcatcc ccaaggcccg taataccgtc ctcagtggtg atttttaatg tgacgaaatt
     9421 acgccccgga caggtaacaa aaacttcagc ctttacgatc ttcatgttcg attccttgca
     9481 tcgcttgtcg tgatgcatga aatctacgca actgagctac taccatacaa gtatgaagat
     9541 caaaaaaagc cggagtgatc acaaaaaaag gcgtatattt gcgccgtgaa tggttgacaa
     9601 aagatgaaac agaatacctt ttgtcagctg acacttcctc gtatcttatt gataaaatgg
     9661 atttatgttc ctacgtgcgc ccccagcccg caacaatgat caacatgccg caaagcgcaa
     9721 ttaacgcccc cgtccagtca taaagagtca gtttcacgcc atccacaacg cgcagccaaa
     9781 tcaacgccgt gcagacataa acgccaccat aagccgcgta aacacgccca ctcgccgcag
     9841 gatgcaacgt taacaaccag acaaacagcg ccagtgaaat ccccgccgga agcaacagcc
     9901 agatactggc gtttcgcttc aaccacagcc agggcaaaaa gcatccaata atttcacaca
     9961 gcgcagtagc aaaaaatagt aacgttgttt taatcatctt tgtcacttat tgacatcacg
    10021 tatagttata gggcgacata atatcatcaa tataaacacc ctccttgaac gttttgcgtt
    10081 cgcagtggat ggtgtagaat cagctttaat cattcataca gggaatgaat tatgaaaatc
    10141 actctcagca aacgaatcgg cctgctcgct tttctgctgc cttgcgcact ggcattgagc
    10201 acaactgttc atgccgaaac taacaaactg gtgattgagt ctggcgacag tgcacaaagc
    10261 cgccagcgcg ccgctatgga aaaagagcaa tggaatgaca cgcgtaatct gcgccagaaa
    10321 gtgaataaac gcactgaaaa agagtgggat aaagccgacg ctgcttttga taaccgcgat
    10381 aaatgtgagc aaagcgccaa catcaatgcc tactgggagc caaatacttt gcgctgcctg
    10441 gaccgtcgaa ctggccgcgt tattatcccc taacctgtta ttgatttaag gaatgtaagg
    10501 acacgttatg ccaagcgccc acagtgttaa gctacgcccg ctggagcgtg aagatttacg
    10561 ctatgtacat caactcgaca ataacgccag tgtgatgcgt tactggtttg aggaacccta
    10621 cgaagccttt gttgaactct ctgatctgta tgataagcat attcacgatc agagcgaacg
    10681 gcgctttgtg gtggaatgtg acggcgaaaa agccggtctg gtggagctgg tggaaattaa
    10741 ccatgttcat cgccgcgcag aatttcagat aattatctcc ccggagtatc aggggaaagg
    10801 tctggcaacc cgtgccgcca aattagcaat ggactatggc ttcaccgttc tcaatctcta
    10861 taagctgtat ctgatcgttg ataaagagaa tgaaaaagcg attcacattt accgcaagct
    10921 tggctttacg gttgaaggtg aattgatgca tgagttcttt attaatggtc aatatcgtaa
    10981 tgccattcgc atgtgtatat tccagcatca gtatctggct gagcacaaaa caccgggtca
    11041 gactctcctg aagccgaccg cacaatagta ttaataataa tcgatcgtat ttttgatggt
    11101 gtaaacccgt tcgacggcgg gttttactcc ttcatcaaca atgattagct gacagtccac
    11161 cggattggcg tgattgtcat attcacagct ctgttttaca tttccaaccc gttgattatt
    11221 cagtaaagta accgctgtgt aatctaattt tttgatcggg tccgttgatg gcgtggcgct
    11281 gacagataat gttttatcgt tacttttcgt ggttttaccc agcggatacc cctgatcatc
    11341 atagcgatat tccatctgca tctgtttgct gctggcttta atcacgaaac cattatcatc
    11401 ggtttcccaa ctcaccccgg cagaaggtaa ttctgctagc tggcattttc cctgcaaacg
    11461 aactcttttc tccagcgtct cggcatcacg gtaataattg gcgtccagta ccagggcaac
    11521 cacggtatta ttttccagat ccagtaattc gagagaatca aaacagcctt cttccgacaa
    11581 agtccccgaa acacgtttcg tcacttcacc ttgctcatcc attaatgtct gagtgaaatc
    11641 ttttacggga ccacgcagcg gatcaaaatc gaattcatta gagaaactgg ccatctccgg
    11701 ggtaaatgaa agtgttacct ctgtgcggtc acatcctgtg agtaatatcg cgagtaagca
    11761 tggcagtaat ttgtatttca caacagtcac ctgagagtag agatgattct caatcatagt
    11821 agcaaataca gtactttaca cgttaaatgc tatgcttaaa gaagttatct tcgcgtaagg
    11881 agcttatgat gaaactctca acctgctgtg ccgcgcttct gctcgccctc gcctcacccg
    11941 cggtactcgc cgcgccggga tcctgtgagc gcatacagag cgatatatca cagcgcatta
    12001 tcaataatgg cgtacccgaa agcagcttca cgttaagtat tgtacccaat gaccaggttg
    12061 atcagcctga ttcccaggtc gtcgggcatt gcgctaatga tacgcataaa attctctaca
    12121 cccgtaccac cagcggtaac gtctctgctc ccgcgcagtc tacccaggat aacgcgcctg
    12181 ctgaaccgca gtaacacatt atcgactgaa cgccggatac gacaggtctg gcgtgtggta
    12241 ttgttatgcg caggtcttgg atgctggctt tgtccgctca ttcgtcgccg tgaacctcac
    12301 gttgccggac ttgttctggg gttaatcctc attcttggcg gtgagatgat cgggcgtgtg
    12361 ctcttttatg gcctgcacat gacggtagga atggctattg caggttaaca aattggtgcg
    12421 gggcaacccg cacatttcag gatgttagga atgacccatt tttcacagca agataatttt
    12481 tctattgcgg cgcgcgtatt gggcgcgctg ttttattacg ctccagagag tgcagaagcc
    12541 gcacctctgg ttgcggtact cacccgtgac ggctgggaaa ctcagtggcc tctaccagaa
    12601 gcgtcattag cgccgctggt gacggctttt cagacacagt gtgaagagac tcacgcccag
    12661 gcctggcagc gtttgtttgt cggcccgtgg gcactgccgt ctccgccgtg gggatcggtc
    12721 tggctggatc gcgaatctgt gctgtttggc gattcaacat tggcacttcg tcagtggatg
    12781 cgcgagaaag gcattcagtt cgaaatgaag caaaacgaac cggaagatca ttttggttca
    12841 ctgctgctga tggctgcgtg gttggcggag aatggtcgcc agacagaatg cgaagaattg
    12901 ctggcatggc acctttttcc gtggtcaaca cgttttcttg acgtttttat cgaaaaagca
    12961 gaacaccctt tctaccgtgc actgggtgaa ctcgctcgct taacgctggc gcaatggcaa
    13021 tcacaactgt tgattcctgt cgcggttaaa ccgctgtttc gataggatca ccgtattatt
    13081 gccggatggt gacgattaca cgcaattcgg caattaatag gttacagtgt cacgtttttt
    13141 tatctcttaa agcacgcact gcttttgcgg ctggcctctt ttgccgcaaa atagtcgccc
    13201 gtgtttcatt gcccatttct gctcatgcat catctacaca tctatccgga tctgcgcact
    13261 atgttccgcc gtctgcttat cgcaacaatc gtcggtattc ttgcggcctt tgccgttgcc
    13321 gggtttcgtc atgcgatgct gctactggag tggctgttcc tcaataatga ctccggcagt
    13381 ctggtcaatg cagcgacaaa tctttccccg tggcgacggc tgctaacccc ggcgctcggc
    13441 ggactggcgg cgggtttgtt gctgatgggc tggcagaaat ttacccagca acgccctcat
    13501 gcgccgaccg attacatgga agcgttgcaa accgatggtc agtttgatta cgcagcaagc
    13561 ctggttaaat cgcttgcctc tctactggta gtaaccagcg gcagtgcaat tggtcgcgaa
    13621 ggtgcgatga ttcttttagc tgcccttgca gcctcgtgtt ttgcccaacg tcttacgcca
    13681 cgccaggagt ggaaattatg gatcgcctgt ggagctgcgg cgggaatggc tgcggcctat
    13741 cgcgccccgc ttgctggcag tttatttata gccgaagtgc tgtttggcac tatgatgttg
    13801 gcctctctcg gcccggtgat tatttccgcc gtcgtggcgt tgctggttaa caatcttatt
    13861 aatcatagcg acgcgttact ctacagcgta caactctcag tgacggttca ggctcgtgac
    13921 tatgcgctga ttatcagtac aggtgtgctg gcaggtctgt gcggaccact gttgttaacg
    13981 ttaatgaacg cctgtcatcg tggatttgtg agtctcaagc ttgcgccgcc ctggcaactg
    14041 gcactgggcg ggttgatcgt gggtctgctt tccctgttca cacctgcagt gtggggcaac
    14101 ggctatagca ccgtacaatc ctttttaacc gctccgccac tgttaatgat cattgccggg
    14161 atcttcctct gtaaactgtg tgccgtgctg gcgagtagtg gttctggagc gcccggtggt
    14221 gtctttacac cgacgctatt tatcggtctt gccattggca tgttgtatgg tcgtagcctg
    14281 ggattatggt tacctgatgg cgaagaaatt acgcttttac tcggattgac agggatggcg
    14341 acactgttgg cggcaacaac gcacgcgccg attatgtcga cgttgatgat atgtgaaatg
    14401 accggggagt atcagctact ccccggttta ttgattgcct gcgtaattgc gtcggtaatt
    14461 tcgcggacgt tacaccgtga ctctatctac cgccagcaca ctgcgaagca tagctaagcg
    14521 gatgtattgc cccagttcac gctgttcggc gcgcggcaga taaggcagtt caccaatgag
    14581 cggtgccgga agttttttac cgagcacatc aatgatttcc gcataatgcg ccagtcctgg
    14641 gttgattcgg ttagccaccc agccaatgag cggcagcccg tcgttggcaa tcgcctgagc
    14701 tgttagcagt gcatggttaa tgcaaccttc ctgaataccg acaaccatca acaccggcag
    14761 ttgttcctgc accacccatt cagagagcgg acgcaaatca ttcatcagac tgcgccagcc
    14821 gccagtccct tctaccacga catgatcgac tttatcggtc aggtttgcca ggccgtttga
    14881 aatgagggtg taattgattg ggcaactgtg cgccacgcta ctttcttctt cgcttaacgc
    14941 gataggatta actgcttcat aaggcagttc gatggttgaa acactctgca acaccagggc
    15001 atctttatta cgcagccctt cgggtgtctc tttgctcccc ttcgctacgg gtttatatcc
    15061 cgcaaccgtt tttccctggg aggctaacgc ttgtagcaat gcgcgggaaa ccaccgtttt
    15121 ccctacagaa gtgtctgtac cggtaataaa gaaacgcttc agcatcacta actccaccgt
    15181 tatgcttcac aaatataaac caggaaaata attaaccttg aaagtctaag ttatgctttc
    15241 ctggcccaaa ttgagatagc gcaaattttg gtagaacagt taaaaaatgt taaccctgca
    15301 acagacgaat caacaaagaa ccgttataca tcgcgtcttt taccagcgca gcgcctgcca
    15361 tcgtgccctg gttagaaaac tgagtactct caacgctaat gtgctgacta tacgcaggaa
    15421 gggcctgctg acggatgctg tccgagatga ctgggaagag gatatctgcc gctttactta
    15481 acggtgagcc aatcagtatt ttttgtgggt taaataaatt caccatgatg gcaagaatgc
    15541 gaccgacatg cgcgcctacc ccggtaatga tgtcttttgc cagtagatcg ccgcgcaatg
    15601 ccgcctgaca caatgagtcc acggttaacg gttgtccatg taacatcgag ctcatggatt
    15661 gattgagacg cagctgtgcc agctcaagaa tactgtccac actggcgatg gtttcgaggc
    15721 aaccgtgatt cccgcaataa cagcgtttcc catacgggtc gacctgtgtg tggcctattt
    15781 ccacgaggct actgctgccg gcgtgtagca gatgaccatc ggtaatgacg cccgccccca
    15841 cgttgtgatc gataaccacc tgaatcacat cgcgcgcccc gcgtgaggca ccaaacaagg
    15901 cctctgccat cgtccatgcg ctgatatcat gctggatata aaccggtacg ccggtatgct
    15961 gctccagcgc ctcgccgagc ggcatctctt ttacatcctc gtagaacggc atgcgatgta
    16021 caatgccatt ttccgtatca ataattcccg gcaaggttat ggcaatcgaa gtcagacgct
    16081 caagtttttt ctggtggcgg ataaaaaact gatcgatatg ggaaatgata cgctccagca
    16141 atggcgagtc atcttttaac gccagttcct gcgactcttc cactaccagt ttgctgctca
    16201 gatcgcgcaa agcaaggaaa atctccccgc gactaatgcg cagagaaaga tagtgccagg
    16261 cttcagtttc aaccaccagc cccactgccg gacggccacg gttccccgct tctttgattt
    16321 ccagctcttg caccaggtgt gcttcgagca tctcacggac aattttagtg atactggcag
    16381 gagccagttg cgccagacgg gaaagatcga tacgcgagac tggaccaagc tgatcaatca
    16441 ggcgataaac cgcgcccgcg ttggtctgct ttatttgatc aatgtgccca ggctgatttt
    16501 cagcaaccac cgcatactcc ctatattttc gcgctccgaa ataatctgta ggctatggtg
    16561 aagcacttca atacgtgtcg tcaaattttt acttaggcat gtgattaaca gcacattttt
    16621 cgggcttttt cgctgaaatt tccctcacct gagagcattc agcagatgaa tacgaaagtt
    16681 acgcgcagcc ggactttgtt catgatgttt cggccagacc aaccacattt cagaaaccgc
    16741 atcctcttca gcaatcggca cccagcgcat ttcgttgagc tgaacacgtt taaatgacgc
    16801 aggcaaaatt gaaacaccca gaccggcgga aaccagaccg atgatggtca ttgcctcacc
    16861 cacctcctga gtgatgacgg gcgtcaaatt gtaacgtctc atcagcccga gaatatcgtc
    16921 atacagccct gttccgacgt gcggatcaaa aaagacaaag ggttctttcg ccagttcagc
    16981 cagcgttaca ctcgggttat ttgccagggg atgatcgtgc gggatcatcg ccataagcgg
    17041 ttcatggacg atgactgcgt gttcaagcgt ctccggtaac gctgtgttac gcagcaatcc
    17101 catatccagc gttccttcaa tgagcggagc gatttgctcg cgagtgttca tttcgcgggt
    17161 ttgtaaatgg acatcgggat aatcacggcg aaacagcgat aacgtatcgg acaccgcccg
    17221 aataaaagga gcggacgaag taaaaccaat gcgcaactcc cccgcttcgc cctgatgcag
    17281 cctttcagcg cgagcggcag catcatccac catagacagg atttgccgac tatctacaag
    17341 aaactgtttt cctgcttccg tcagcaatac actgcgattg gtccgtgcca gcagtcgggc
    17401 accaatttgt tgctccagcg cctgaatctg ctgacttaga ggtggttgcg aaatattcag
    17461 gcgggcagcg gcacgcccga aatgcagctc ttctgcaaca gcgacaaagt aacgcagatg
    17521 acgaagttca atattcatat ttaaaacatc ttatttgaga ttattaatat attagacaga
    17581 acaattcgat tttcctaatc tattcataag cctgatccac aggcatattt agcaaggatt
    17641 tcaagtgagc cgtactacaa ctgttgatgg cgctccggca agcgacactg acaagcaaag
    17701 catttctcag ccaaatcaat ttattaaacg cggtacgccg caatttatgc gcgtcaccct
    17761 ggcgcttttc tctgccggac tggcaacctt cgcacttctc tattgtgtgc aacctatcct
    17821 tccggtgctt tcgcaggagt ttggcttaac gcccgcgaac agtagtattt cactgtccat
    17881 ttccacggcg atgttggcta tcggtttgct gtttactggc ccgctatccg atgccattgg
    17941 tcgcaaacca gtgatggtca cggcgctact gttggcctcc atttgtacgt tactttcgac
    18001 aatgatgacc agctggcacg gcattttgat tatgcgcgcc ttgattgggc tttcgttaag
    18061 tggcgtggca gccgttggca tgacttatct tagcgaggaa atccacccca gtttcgtggc
    18121 cttttcgatg gggttgtata tcagcggcaa ctcaattggc ggcatgagcg gacgcttaat
    18181 tagcggtgtc ttcacggact ttttcaactg gcgaattgcg ctggcggcaa tcggttgttt
    18241 cgcgctggcc tcggcgttga tgttctggaa aatcctcccg gaatcacgcc attttcgccc
    18301 gacttcgctg cgacctaaga cgttgtttat caactttcgt ctgcactggc gtgaccgggg
    18361 attaccgtta ttgttcgcag aaggcttttt gctgatgggg tcgttcgtca cgctgtttaa
    18421 ttacatcggc tatcggttga tgctctcccc ctggcatgtc agtcaggccg ttgtcggctt
    18481 attatcgctg gcctatttga ccggtacatg gagctcaccc aaagccggaa ccatgagcac
    18541 ccgctatggg cgcggtccgg tgatgttgtt ttcgacgggg gttatgctgt ttggtttact
    18601 gatgacctta ttcagctcgc tgtggctgat ctttgccgga atgttactct tctcagccgg
    18661 attcttcgca gcccactccg ttgccagcag ctggatcggc ccccgcgcaa aacgcgctaa
    18721 aggccaggcc tcctcgctgt atctgttcag ttactatctg gggtcgagta ttgccgggac
    18781 gctgggtggt gttttctggc ataactatgg ctggaacggc gtcggtgcat ttattgctct
    18841 gatgctggtc attgctctgc tggtcgggac gcgtttgcat catcgtctgc acgcctgaaa
    18901 aaataagtcc ggactgaggt aaatacccgt ccggacttat tgcctgctca aaccaacgtt
    18961 aatagccatc ctaaaataga cgaagcgcca gccaattccc gcagcgcatc tagcgtcatc
    19021 aggattataa gtacccaaat aaacggattc attttgctgt gtgtcattta ttactgatgc
    19081 gcagttattc taccgctttg taagtagtaa aatagttaac acgatcaaga ctactattat
    19141 tggtagctaa attttcctta agtcacaata cgttattatc aatgctgtaa tttattcagc
    19201 gtttgtacat atcgttacac gctgaaacca accactcacg gaagtctgcc attcccaggg
    19261 atatagttat ttcaacggcc ccgcagtggg gttaaatgaa aaaacaaatt gagggtatga
    19321 caatgaaaaa agtattagct ctggttgttg ccgctgctat gggtctgtct tctgctgcct
    19381 ttgctgcaga gactgcgacc acacctgctc cgactgcgac gaccaccaaa gcagcgcctg
    19441 cgaaaaccac acatcataaa aaacagcata aagcagcacc tgcccagaaa gcgcaggcgg
    19501 ctaaaaagca tcataaaaat gccaaagctg aacagaaagc ccctgaacaa aaagcgcagg
    19561 cggcgaagaa acacgccaag aaacacagcc atcagcaacc ggcaaaacct gctgcacaac
    19621 ccgcagcgta agttttcaac agtaatgctg gcgcgccccc tcgcgcctga aaattacggt
    19681 gctaagcggg taacgtttag caccgccttt agcggagggt agtatggtgg gccgttatcg
    19741 ctttgagttc attcttatca tccttatttt atgcgcactg attgccgccc gtttttatct
    19801 ttcctgatcg tagttatctg attttactcc cactttcatc ccgtcccgtc tatagtattt
    19861 atgagggttt gcttttaata atcataatta cccaccagag tgtgatatgc gtacaaccat
    19921 tgctgtagtg ttgggtgcaa ttagtttgac gtctgctttt gtgtttgcag ataaaccaga
    19981 cgttgccaaa tcggcaaacg atgaggtcag caccctgttt tttggtcatg atgatcgtgt
    20041 gccagtgaat gacacgaccc aatcaccgtg ggatgcggtt ggccaactgg aaacggccag
    20101 cggcaattta tgtacggcga cactgattgc acccaatctg gcattaacgg caggacactg
    20161 tttattgacg ccgccaaagg gtaaagccga taaagcggta gcgctgcgtt tcgtgtcaaa
    20221 taaaggtctt tggcgctatg agatccacga catagaaggc cgcgttgatc cgacactggg
    20281 aaagcggtta aaagcagatg gggatggctg gattgtacct cccgcagctg cgccctggga
    20341 cttcggtttg attgtgctac gtaatccccc ttctggcatt acaccgttgc cgttatttga
    20401 gggagataaa gccgcgctta ccgccgcatt aaaagcggct ggtcgtaaag tgactcaggc
    20461 gggctaccct gaagatcatc tcgatacgtt gtacagtcat caaaactgtg aagtgactgg
    20521 ctgggcgcaa acgtcggtga tgtcgcatca gtgcgatacc ttgccgggtg acagcggttc
    20581 gcctctgatg ttgcataccg atgacggctg gcaattaatt ggggtgcaaa gctcggctcc
    20641 tgccgcgaaa gatcgctggc gcgccgataa ccgggccatt tctgttaccg gttttcgcga
    20701 caagctggat caactgtcgc aaaaataatg ttcaaacgct gcccgacagc gcgggcagcg
    20761 tgttcatcag gcaagtttca ccatgatcat tccagccaac agcaagacca ggccaatcca
    20821 gcctttacga tttaaccgtt gaccaaacaa gatccaacct gcggctaacg tggcggcaat
    20881 accaaacccg ccccacaatg cataagcgac agacaagtcg atccctttaa cggcctgaga
    20941 aagcgcacta aaggcagcca gcaccgccgc cagagagagc aagccaaata ttttgcgacg
    21001 aaagccgtca gaaaatttca aaaagacgtt agcaatgatt tccagcacga ttgccaatgc
    21061 cagccaggcg gcgtgaaccc attcaaactg cgccatggtt cacctccagt tcaggtttac
    21121 gcgctttacg ggtacctgat ttgatcaata caatcccggc gaccagggtg gttaacccgg
    21181 caattttcat cagcgataaa ctttcgtcga ataacaaaac gctaaacaag gtaataaata
    21241 aaataccgat accttcccac agcgcataag ccacgcctaa ggcgattttt ttaacggcga
    21301 aagagagaaa tatatacgac agagaaatca tcaccagcat taaaataaag ccgccatttc
    21361 cctcactgac gctcgcccat ttcattgaca gcgtaccggt aatttccgta gcaatagcca
    21421 ggcctaataa aatccaataa atatacattg tccttctcct gcaagagaat tattttaatt
    21481 ttcgcttaat tcagcgaaac caaagtaaag tgatcaagcc ccgcgcatag cgcaagctta
    21541 ggcagaagaa aggactaaag cgcgttgcgc cagtgctgct cacctacgag caagatagtg
    21601 gatgaggtac gaagatgggt aaatgtagaa aataacgtcc tgaacaaatt gtccataata
    21661 ttacaattat ccgcagtgtt gcttctcgtc atcgcggatg ataattgtcc tcggtagttg
    21721 aacacgcctg atttgtatca tagcttaaga attaactcaa aatattttca cttctttacc
    21781 tgagcggttt gattttcgtt atgatgatgg agcgaaaaag acattattat tagcaaagaa
    21841 gaaaaaacgg ggacaagcat ggcaaagccg atcatcacgc tcaatggcct aaaaatcgtc
    21901 attatgttgg gaatgctggt cattattctc tgcggtatcc gttttgccgc cgagattatc
    21961 gtgccattta ttctcgcatt atttattgct gttattctta acccgctggt gcaacacatg
    22021 gtccgctggc gtgtgccgcg tgtactggca gtgtcgattt tgatgaccat catcgtgatg
    22081 gcgatggtgt tgctgttagc ttatctgggt tccgcgctca atgagttgac gcggacgtta
    22141 ccgcaatatc gcaactctat tatgacgccg ctgcaagcga ttgaaccgtt gttgcaacgc
    22201 gtagggattg atgtctcagt tgaccagctg gcgcattaca ttgatccgaa cgcggcgatg
    22261 acattgctca ccaacttatt gacgcagtta tctaatgcca tgtcatcaat atttttattg
    22321 ctgctgacgg tgctgtttat gttgctcgaa gtgccacaat tgcccggaaa atttcagcaa
    22381 atgatggcgc gtccggttga agggatggcg gcgattcagc gtgcgattga cagtgtgtct
    22441 cattatctgg tgctgaaaac agccatcagc atcatcaccg gcctggttgc atgggcgatg
    22501 ctcgccgccc tcgatgttcg cttcgctttt gtctggggat tgctggcctt tgcgcttaat
    22561 tacatcccta atattggttc agttctcgcg gcaatccccc ctatcgctca ggtactggtg
    22621 tttaatggct tctacgaagc gttgctggtg ctggcgggat atctgctgat taatcttgtc
    22681 ttcggcaata ttctggagcc gcgcatcatg gggcgtgggc tggggctttc cacattggtg
    22741 gtatttttgt cgttgatttt ttggggatgg ttgttaggac cggtgggtat gctactttcc
    22801 gtgccgttga caattattgt caaaattgcg cttgaacaaa cagcgggagg tcaaagcatc
    22861 gccgttctgt taagcgatct caataaagag tgacggcctc cgcagaggcc gtcagggtta
    22921 cagagccttc aggattgcat ccacgctggc tttggcgtca ccaaacagca tgtgggtgtt
    22981 ttccttgaag aacagcgggt tttgcacacc agcatagcca gtgttcatcg aacgtttaaa
    23041 gacaatcacg ttctgcgctt tccacacttc cagcacaggc ataccagcaa tcggactctt
    23101 cggatcatcc tgcgccgccg gattaaccgt gtcgttagca ccaatcacca gtacggtatc
    23161 ggtatcagca aagtcatcgt tgatctcgtc catttccagc acgatgtcat acggtacttt
    23221 tgcttcagcc agcaatacgt tcatatgtcc aggcaaacgc cccgcaaccg ggtggatacc
    23281 gaaacgcaca ttaataccac gagcgcgcaa tttctcagta atttcagcga caggatattg
    23341 cgcctgcgca actgccatgc cgtaccccgg agtaatgatc actgaatggg agtttttcag
    23401 cagttccgct gtctcttctg cagtgatttc gcggtgctcg cccacttcct gatcatcacc
    23461 agtagaagag ccgtcggtgc cgaaaccacc cgcaataacg ctgataaagg aacggttcat
    23521 cgccttacac ataatgtaag aaaggatagc ccccgaagaa ccgaccagcg caccggtcac
    23581 aatcagcagg tcgttgctga gcataaagcc cgcagccgca gccgcccagc cggagtacga
    23641 gttcagcatc gacaccacca ctggcatatc tgcaccaccg atggaggcga ctaaatgcca
    23701 gccgaatacc agcgcaatgg cggtcattat cagcaatgcc agcacttgca ggccgacgct
    23761 atccgtgcga acaaatacaa tcagcagcag gaaggaaacg accagagccg ccaggttcat
    23821 tttgtgacgg tttggcagca tcaacggttt agacgaaatc ttgccacaca gtttgccgaa
    23881 cgccaccacc gaacccgtga acgttaccgc cccgatgaag ataccgagga acacttccgt
    23941 caggtgaata ttgaccagaa tcggtgccat tcccgcgtca tgatgcagat agctgttaaa
    24001 accaaccagc actgccgcca gacccacgaa gctatgcagg atcgccacca gttctggcat
    24061 ttcggtcatt tcaactttct tcgccagacg gataccaatt gccccaccaa tgaccatagc
    24121 cagcaagatc cagccaacat tacccgtatc cggtccaaaa atggttgcga ttaacgcaat
    24181 cgccatcccg gcgataccga agttgttacc ctggcgagac gtttcatgtt tcgaaagacc
    24241 ggccagactg aagataaaca ggatcgcggc aacaatgtat gcagctgtaa ctaatcctcc
    24301 agacatatgt taccccttaa tttttgcgga acattttcag catgcgctga gtcacggtga
    24361 agccaccgaa aatattaatg ctggctataa gcaccgcgat aaaactaagg aagctaaccc
    24421 agccgccctg gccaatctgc aacagtgctc cgacaacaat aatccctgaa atcgcgttgg
    24481 tgaccgacat caacggtgta tgcagcgcgt gcgatacatt ccacaccacg taataaccga
    24541 caacgcaggc cagcgcgaac acagtgaagt gtccaaggaa ctcttttggc gcaacgcttg
    24601 ccatccagcc gaaaaggatg attgccaacg ccatcaacgc gtatttacgc cacggtgagc
    24661 aggcacattt ttcctcagtt ttcacttccg gtgccgcttt ttgtgccgcc tgcggctgag
    24721 ctgatacctg aatcggcggt gccggccagg taatttcgcc cgcacggatc acagtcacgc
    24781 cacgaatcac cacatcatca aaatccacag tgatgttgcc gtctttctct ttgcacaaca
    24841 gtttcagcag attaacgagg ttagtgccgt aaagctgtga ggattgcgtc ggcagacggc
    24901 ccggaagatc ggtgtaacca attactttga caccattttc cgtagtgaag atttcacccg
    24961 gcacggtgta ttcacagttg ccgccgtttt gggctgccag gtcgacaatc acactgcccg
    25021 ccttcatgga gtcaaccatt tcacgggtaa ttagcttcgg cgctggtttg cctggaataa
    25081 gcgcggtggt gacaatgata tcgacctctt ttgcctgggc ggcaaagagt tccatttccg
    25141 ctttgatgaa cgcgtccgac atcactttgg catagccatc gccgctgccc gcttcctctt
    25201 taaaatccag ctcgaggaat tctgcgccca tactttgaac ttgttctttc acttccgggc
    25261 gggtgtcgaa tgcacgcaca atcgcgccga gactgtttgc tgcgccaatg gcggccagac
    25321 ctgcgacacc ggcaccaatc accatcactt ttgccggtgg cactttcccg gccgcagtaa
    25381 tttgtccggt aaagaagcgc ccaaattcat gtgccgcttc aacaatggcg cgataaccgg
    25441 cgatattcgc catcgagctt agtgcgtcca gcgattgtgc gcgtgagata cgcggcacag
    25501 agtccatcgc catcacggtc acgttacgtt ccgcaagttt ttgcattaat tccggattct
    25561 gcgcaggcca gataaaactc accagcgttg tcccaggatt cagtaacgca atttcatcat
    25621 ctaacggcgc attgaccttc agaatgatct ctgactgcca gacgctattc ccttctacga
    25681 tttcagcgcc cgcttgcaca aacgctttat cgtcaaaact tgccagttga cccgcgccgc
    25741 tctctaccgc gacggtaaaa cccagtttca gcagctgttc cactgttttt ggcgttgctg
    25801 caacacgggt ttcattggtt aaccgttctc ttggtattcc aattcgcatg atattccctt
    25861 ccatcggttt tattgatgat ggtttgcttg tgtcaggagc cacacaagcc gctcatgtac
    25921 gagctaaatg ttactccgtt aaaataaatt agtaacaaac gccctataac gtactgaaaa
    25981 ttatgcctgt gatctagcgc caaaaatcag tatttcggcg tgaactcgca aaatattaac
    26041 gattcagccg tgatagtggg ataaacacct tagaacgccg gataaagact gataattgtc
    26101 ttcggcggcc gggtaaaacg agacatcgcc ccggcacaaa tcactactta acattaaatt
    26161 aacttataca attcagttgc ttcagtagta ttgatggtga tacggctgtt ttttaagcat
    26221 agcggtcatt tgagcagaat taaaattggt ttaaggaatg tgatatgaaa aatgacgcag
    26281 acagttacac cgtttaaatg caataatcag ccacgtttct cgttaataac aataccagta
    26341 cctggtttgc gcaaggcgaa ggattatttt tatgaagctt aagaacaccc tcctggcgtc
    26401 ggcactgctt tctgctacgg cattctccgt taacgcagca acagaactga caccggagca
    26461 agcggcagcg gttaaacctt ttgaccgtgt agtgattacc ggtcgtttta atgctattgg
    26521 cgaagcggtg aaagccgttt ctcgtcgcgc agataaagaa ggtgccgcct ctttttatgt
    26581 tgtcgacact tctgattttg gtaacagcgg taactggcgt gtggtcgctg acctctataa
    26641 agccgatgct gaaaaagcag aagaaacaag taatcgcgta attaacggtg ttgtcgaact
    26701 gccgaaagat caggctgttc tgattgaacc gtttgacacg gtcaccgtcc agggcttcta
    26761 tcgtagccag ccagaagtca atgatgccat caccaaagca gcaaaagcga aaggtgccta
    26821 ctctttctac atcgttcgtc aaatcgatgc caaccagggc ggcaaccagc gtattactgc
    26881 attcatctat aaaaaagatg ctaagaaacg tatcgtccag agcccggatg tcatcccggc
    26941 agattccgaa gcaggacgtg cagctctggc tgccggtggc gaagctgcga agaaagttga
    27001 gatcccgggt gttgcgacta ccgcatcacc aagttctgaa gtcggtcgct tctttgaaac
    27061 ccagtcatca aaaggcgggc gttacaccgt cacgctcccg gatggcacta aagtcgaaga
    27121 actgaacaaa gcgacagcag cgatgatggt cccgttcgac agcatcaaat tctctggcaa
    27181 ctacggcaac atgaccgaag tctcctatca ggttgcgaaa cgtgccgcga agaaaggtgc
    27241 taagtattac cacatcaccc gccagtggca ggaacgtggt aataacctga ccgtcagcgc
    27301 agatctgtat aaataactat tcagttactc ctataaggcg gcttgatgcc gcctttcgca
    27361 tttttagtca taaatttccc tgcgaccatt gcatgcagac acatctcttc gtaaaatccc
    27421 gcgcttcagt ggcaaactcc atttttatgc gttttgccct aatatttatt cttcttgtca
    27481 cgttttaatc actggatatc gatggaaaag aaactgggac tgagcgcact caccgcgctg
    27541 gtattaagct caatgctggg cgcgggtgtt ttcagtctgc cgcaaaatat ggcggcagtt
    27601 gccagcccgg cagcactgct catcggctgg ggtattactg gcgctggcat tttattgctg
    27661 gcctttgcca tgctgatcct cacgcgcatt cgccccgaac ttgacggcgg tatctttacc
    27721 tatgcccgcg aagggtttgg cgagctaatc ggcttttgtt ccgcatgggg atactggctg
    27781 tgcgcggtca tcgccaacgt ctcctatctg gtgatcgttt tttccgcgtt aagctttttt
    27841 acggacacgc cggaattgcg cctgtttggc gatggtaata cctggcaatc gatcgtcggt
    27901 gcatcggctt tattgtggat tgttcacttt ttgatcctgc gcggggtgca aaccgctgcc
    27961 agcattaacc ttgtggcgac actggcaaaa ttgttgccgt tgggtctgtt tgttgtgctg
    28021 gcgataatga tgttcaaact ggataccttc aagctcgact tcaccggact tgcacttggc
    28081 gtacccgtgt gggaacaggt gaaaaacacc atgctgatca ccctgtgggt tttcattggt
    28141 gtggaaggtg ccgtggttgt ttctgcgcgt gcgcgtaata agcgtgatgt tggcaaagcg
    28201 acactgctgg cggttctctc cgctctgggc gtttatttgc tggtaacgct gctttcgctg
    28261 ggcgtggtgg cacgtcctga actggctgaa attcgtaacc cgtcaatggc cggtctgatg
    28321 gtggaaatga tgggaccgtg gggcgaaatc atcatcgctg ccggtttgat cgtttccgtt
    28381 tgcggcgcgt acttaagctg gaccatcatg gcagcagaag ttccgttcct tgcagccact
    28441 cataaagcat tcccgcgcat ttttgcgcgc cagaacgcac aagctgcgcc atccgcttca
    28501 ctgtggctga ccaacatctg cgtgcaaatt tgtctggtgc tcatctggct gaccggttcc
    28561 gattacaaca cattgctgac catcgcctca gaaatgattc tggtgcccta tttcctggtc
    28621 ggcgcgtttt tgctgaaaat cgcaacccgt ccactgcata aggcggtagg tgtcggtgcc
    28681 tgcatttatg gcttatggtt actgtacgcc tctggtccaa tgcacttgct gttatccgtt
    28741 gttctgtatg cgccggggct gctggttttc ctgtacgcgc gtaaaacgca tacacatgat
    28801 aatgtactga accgtcagga aatggtgctg attggcatgc tgttaattgc ctcagttccg
    28861 gcgacctgga tgctggtggg ataacgttca tcccatcgtt ggatagcaaa ggagataacg
    28921 atgggtaaag cccaaccctt gccaatatta attactggcg gtggtcgtcg catcggcctc
    28981 gccctcgcct ggcatttcat taatcaaaag caaccggtga ttgtcagcta tcggacgcac
    29041 tatcccgcca ttgatgggct gatgaatgcg ggtgcgcagt gtattcaggc tgatttttcg
    29101 accaacgacg gtgtgatggc gtttgccgat gaagtactaa aaagcaccca tggtctgcgt
    29161 gctattttgc ataacgccag cgcgtggatg gcggaaaaac cgggtgcgcc actgaccgac
    29221 gtactggctt gcatgatgca gatccacgtt aataccccat acctgctcaa ccatgctctg
    29281 gaaagattac tgcgtgggca cggacacgcc gccagcgata tcattcactt taccgattat
    29341 gtggtggagc gcggtagcga caaacatatt gcctatgctg caagcaaagc agcactggat
    29401 aatatgaccc gctcgtttgc ccgcaagctg gcaccggaag tgaaagtgaa ttctattgcg
    29461 ccatcgctga tcctgtttaa tgagcatgat gatgccgaat atcgacaaca ggcgctgaat
    29521 aaatcactga tgaaaaccgc gcctggcgag aaagaagtga tcgacctggt cgattactta
    29581 cttaccagct gctttgtcac cggacgcagt ctcccacttg atggcggtcg tcatctgcgt
    29641 taatgcagct taatccagca tataatcaac acccatgcgc ttattcccca gcacgcaaca
    29701 gagccaacaa aagcagcggg caagcgcatc attccggtga aataccatag cgacgccagg
    29761 taaacaaaat agggaataat cgaccacata ctaaaaatga tggttgcgcg taaggcttca
    29821 atgccgcgtt cgctggcaac aatataatgc gcgataagcg caaaggtcgg gaaaagtgga
    29881 atcagcccgg cgatataata atttttcgtt tttgctaaaa caccaatcaa cagcactacc
    29941 agcgcaccga gcgcggcttt gattaccagc cccatctttt taccttaaca cttccataac
    30001 aagtcatcag tagaatacct gatgaaacct tgtttagaaa cgattgatag taagtaaaaa
    30061 cagtgcggtg tattgtgacg tttttatatc taccgtgaat gttatgaaca ctatcgtatt
    30121 tgtggaagat gatgcggaag tcggtgcact gattgccgcc tacctggcaa aacatgatat
    30181 gcaggttacc gtagagccgc gcggcgacca ggccgaagag accattctac gagaaaatcc
    30241 ggatctagta ttactggaca tcatgctgcc cggcaaggac ggcatgacca tttgtcgtga
    30301 tttacgcgca aagtggtccg gaccgattgt tcttctaacc tctctcgata gcgacatgaa
    30361 ccacatcctg gcactggaaa tgggtgcctg cgactatatt ctcaaaacga caccccctgc
    30421 tgttttgcta gcgcgtttac gcttgcattt gcgtcagaat gagcaagcca cagtgaccaa
    30481 aggtattcag gaaacgtctc tgactcccta caaagccctg catttcggca cgttgaccat
    30541 cgatcccatc aaccgcgtag tctccctggc taacactgaa atctcgctct cgacagctga
    30601 tttcgaatta ttgtgggaat tagctaccca tgccgggcaa atcatggacc gcgatgcatt
    30661 gctgaaaaat ttacgcggcg tcagttatga cggactggat cgtagcgtgg acgtggctat
    30721 ttcgcggtta agaaaaaaac tgctcgataa cgccgcagaa ccttatcgta ttaaaaccgt
    30781 gcgtaacaaa ggctatcttt ttgcgcctca tgcgtgggaa taagcgatga aaaaactgtt
    30841 tatccagttt tacctgttat tgtttgtctg cttccttgtg atgtctctgc tggtcgggct
    30901 ggtgtacaaa tttaccgccg aacgcgcagg caaacagtcg ctggatgatt tgatgaacag
    30961 ttcgctgtat ctgatgcgca gcgaattgcg tgagatcccc ccacacgact ggggtaagac
    31021 gctgaaagag atggatttaa atctctcttt cgatctgcgt gtcgagccgc tgagtaaata
    31081 tcatcttgat gatatttcca tgcaccggct gcgtggcggc gaaattgtcg ctctggacga
    31141 tcagtacact tttttgcaac gtatcccgcg aagccactac gtgctggcag ttggtcctgt
    31201 tccttatctt tattacctcc atcagatgcg attgctggat atcgccctga tcgcttttat
    31261 tgccatttcc ctcgcctttc cggtgtttat ctggatgcgt ccgcactggc aggatatgtt
    31321 aaaactggaa gcagcggcgc aacgatttgg cgatgggcat ctcagtgagc gtatccactt
    31381 tgatgaaggt tcgagctttg aacgacttgg cgtcgcattt aaccagatgg cggacaatat
    31441 caacgccttg attgccagca aaaaacagct tattgacggt atcgctcacg aactgcgaac
    31501 accgttagtg cgcctgcgtt atcgactgga gatgagcgat aacctgagcg ccgccgaatc
    31561 ccaggcgttg aatcgcgata tcagtcaact tgaagcttta atcgaagagc tgctgactta
    31621 tgcccgactc gaccgcccac aaaatgagct tcatcttagc gaaccagacc tgccgttgtg
    31681 gctgtcaacg catctggcag atattcaggc agtaacgccc gataaaacgg tacggataaa
    31741 aacgctcgcg caaggccatt atgcggcgtt ggatatgcgc ttaatggagc gcgtgctgga
    31801 taatttgctc aataacgccc tgcgctactg tcactcaacg gttgaaacca gcctgctact
    31861 gtcggggaat agagcgacat taattgttga ggatgatggc ccagggattg ccccagaaaa
    31921 ccgcgaacat atctttgaac cttttgttcg cctcgatccc agccgggatc gctcaaccgg
    31981 cggctgcggg ctggggctgg caattgtcca ctctatagca ctggcaatgg gcggtacggt
    32041 taattgtgac accagcgaac tgggtggtgc ccgcttctcg ttcagctggc cgttatggca
    32101 taacatcccg caatttacct ctgcctgaca ctatgcgcac ggtggtcaag tcaccaccac
    32161 tgtgctataa aataaggatg ttgtaactaa agtggttaat attatggcgc gttacgatct
    32221 cgtagaccga ctcaacacta cttttcgcca gatggaacaa gagctggctg catttgccgc
    32281 tgagctagaa cagcacaaat tgctggttgc ccgcgtgttc tctttgccgg aggtaaaaaa
    32341 agaggatgag cataatccgc ttaatcgtat tgaggtaaaa caacatctcg gcaacgacgc
    32401 gcaatcgctg gcgttgcgtc attttcgcca tttatttatt caacaacagt ccgaaaatcg
    32461 cagcagcaag gccgctgtcc gtctgcctgg cgtgttgtgt taccaggtcg ataacttttc
    32521 gcaagcagcg ttagtcagtc atattcagca catcaataaa ctcaagacca cgttcgagca
    32581 tatcgtcacg gttgaatcag aactcccctc cgcggcacgt tttgaatggg tgcatcgtca
    32641 tttgccgggg ctgattacac ttaatgctta ccgcacgctc accgttctgc acgaccccgc
    32701 cactttacgt tttggctggg ctaataaaca tatcattaaa aatttgcatc gtgatgaagt
    32761 cctggcacag ctggaaaaaa gcctgaaatc accacgcagt gtcgcaccgt ggacgcgcga
    32821 ggagtggcaa agaaaactgg agcgagaata tcaggatatc gctgccctgc cacagaacgc
    32881 gaagttaaaa atcaaacgtc cggtgaaggt gcagccgatt gcccgcgtct ggtacaaagg
    32941 agatcaaaaa caagtacaac acgcctgccc tacgccgctg attgcactga ttaataggga
    33001 taatggcgcg ggcgtgccgg acgttggtga gttgttaaat tacgatgccg acaatgtgca
    33061 gcaccgttat aaaccacagg cgcagccgct tcgtttgatc attccacggc tgcacctgta
    33121 tgttgcagat taacccccgg ctttcatact gccgaccatc tgttctggcc gtacccagcg
    33181 gtcaaactcg gcttcgctaa gataccccag cgcaagggcc gccgctttta aggtcagccc
    33241 ttctttatgc gcttttttgg cgatctcggc ggctttgtca taaccaatgt gggtgttaag
    33301 cgcagtcacc agcatcagcg attcattgag taattgattg attcgctcac gattcggttc
    33361 aatgcccact gcacagtgtt tgttgaaact ttccatgcca tctgccagca agcgcaccga
    33421 ttgcaggaaa ttatggatca ccatcggacg gaagacgttc agttcaaagt taccggaagc
    33481 gccacccatg ttgatcgcca cgtcgttccc catcacctga cagcagagca tggttaatgc
    33541 ttcgcactgt gttgggttca ctttccctgg catgattgag ctgcccggct cattttccgg
    33601 gattgagatt tcaccaattc cgcagcgcgg gccagaggcc agccagcgga catcattggc
    33661 aattttcatc agtgacgcag ccaacccttt caatgcgccg tgcgcctgaa ccagggcatc
    33721 acaggtcgcc agcgcttcaa atttgttcgg cgcggtaaca aacggtgcac aggtaatgac
    33781 tgccagttca tctgctacgc gacgcgcata ttccggatgg gtatttagtc cagtacccac
    33841 cgctgtaccg cccagagcca gttccgctac gtgaggcagg ctgtattcga tatgtttgag
    33901 attatgctcc agcatcgcta cccagccgga aatctcctgc cccagcgtta acggcgtggc
    33961 gtcctgcaag tgggtacgac cgattttgac gatatcggca aatgcgcggg atttttcact
    34021 cagcgtctgg gtcagggttt taagttgtgg aatgagttgc ttgcgcagcg ccagcagcgc
    34081 cgcaacgtgc atcgccgtcg gaaagacatc gttggaactt tggcttttgt tcacgtcgtc
    34141 gttagggtga actttacgtt ccatcccgcg cacgccgccg agtaattcac tggcccggtt
    34201 agccagcact tcgttcatgt tcatgttact ttgcgtgccg gagccggtct gccagatagc
    34261 caacgggaat tcgtcgtcat gctgtcctgc cagtacttca tccgccgcct gccgaatggc
    34321 gctcgctttc tcttcagaca acaagcctaa atcttcatta acttttgccg ctgcgcgctt
    34381 ggttagcgcc agcgcatgaa tcagtgaggt gggcattttc tccgtcgaaa tgcggaaatg
    34441 ctccagcgag cgttgagttt gtgcgcccca cagcttatct gccgggacat caatcgcccc
    34501 catcgaatct ttttcgctgc gtactgtatt catggcctgc tcctcacctg attaattttt
    34561 tctttctgtt ttgctttcgt taagcaactt ttagcttact cattatttac catttgataa
    34621 caaatgtttg gtctttcgtg ccatgtaaaa aaaccgcccc gaagggcggc tctgtttatt
    34681 tcacacagcg ggtgcattgt gttagttgta tctgctggaa gaagtcattt cctttatcat
    34741 ccacaaggat aaacgccggg aaatcttcca cttcaatttt ccagatggct tccattccca
    34801 gttccggata ttcaacacat tccaggctct taatacttcc ctgcgccaat acagctgccg
    34861 gaccaccgat actgccgagg tagaagccgc cgtgtttttt acaggcatcc gtcacctgct
    34921 ggctgcggtt gcctttcgcc agcatgatca tacttccgcc ctgcgcttgc agttgatcga
    34981 cataagaatc catccgtccg gcggtcgttg ggccaagaga accggaggca taaccttccg
    35041 gcgttttggc cggacccgcg tagtaaatcg gatgatcttt aatgtactgc ggcagccctt
    35101 caccgttatc catccgctct ttcagtttgg cgtgagcaat atcacgaccg acgataatcg
    35161 tgccgttaag cgataagcgt gtagaaacgg gatactgcga caactgtgcg aggatctctt
    35221 tcatcggacg gttgaggtca acgcgcaccg cttcgccttc tcctgctttg cgtagctctt
    35281 ccgggatata tttgcctgga ttatgttcca gtttttcgat ccagatcccc tgacggttga
    35341 tcttcgcttt gatattacgg tcagcagaac aggagacgcc cataccgacc gggcaggatg
    35401 cgccgtgacg tggcaggcga atcacgcgga tgtcatgagc gaagtattta ccaccaaact
    35461 gcgcacccag accaagattt tgcgcttcga tcagcaattc tttttccagt tccacatcgc
    35521 ggaacgcctg accgtgctca ttcccttccg ttggcagttc atcatagtat ttagcggaag
    35581 cgagtttcac cgttttaaga ttcgtttctg cagaagttcc accaataacg aacgcaatat
    35641 gatatggagg acaggccgcc gtacccagcg tgcgcatctt ctcaaccagg taatttttca
    35701 gttttcccgg cgtcagtaac gctttggttt cctgatagag atacgtcttg tttgccgaac
    35761 cgccaccttt ggcgatacag aggaatttgt actcgtcgcc atcaacggca taaagatcga
    35821 tctgcgctgg caagttggtg ccggtattca cctctttata catatccagc ggcgcgtttt
    35881 gcgagtagcg cagattatcc tcgatataag tgttatagac accgcgcgcc agcgctgctt
    35941 catcaccgcc accggtccat acacgctgcc cttttttacc aacaataatc gcggtgccgg
    36001 tatcctgaca ggttggcaga acgccttttg ccgcgatgtc ggagttacgc aggaattgca
    36061 gcgccacata tttatcattt tcgctggcct ccgggtcacg cagaatgtcg gccacctgtt
    36121 gttggtgagc cggacgcagc ataaatgacg catcatgaaa cgcctgacgc gccaacagag
    36181 ttaacgcttc gggtgcgact ttcaaaatct cctgcccttc aaattcagat acgctaacgt
    36241 gttcgctggt tagcaggtaa tactcagtat catctttttt gagtggaaaa ggagcctgat
    36301 aatgaaaggg tttgtttgac attgttctct cacttactgc ctggtttggt tatgctctgg
    36361 gcgggtgttc cgttgccctg ttgaaagcga gtaacaatat cctacacact tttttaacaa
    36421 aaactgagac tagtacgact ttttgcggct ccaggttact tcccgtagga ttcttgcttt
    36481 aatagcggga ttaatttcca cattaaaaca gggattgatc atgcaaaaac tcattaactc
    36541 agtgcaaaac tatgcctggg gcagcaaaac ggcgttgact gaactttatg gtatggaaaa
    36601 tccgtccagc cagccgatgg ccgagttgtg gatgggcgcg catccaaaga gcagttcacg
    36661 ggtgcagaat gccgcaggag atatcgtttc actgcgtgat gtgattgaga gtgataaagc
    36721 gactcttttg ggagatgccg ttgccaaacg ctttggcgag ttgcctttcc tgttcaaagt
    36781 attatgcgca gcccagccgc tctccattca ggttcatcca aacaaacgca attctgaaat
    36841 cggttttgcc aaagaaaatg ctgcaggtat tccgatggat gccgctgagc gtaactataa
    36901 agatcctaat cacaagcctg agctggtttt cgccctgacc cctttcctcg cgatgaacgc
    36961 gtttcgtgag ttttccgaga ttgtctccct gctccagcca gtcgcaggtg cacatccggc
    37021 gattgctcac tttttacaac agcctgatgc cgaacgttta agcgaactgt tcgccagcct
    37081 gttgaatatg cagggtgaag aaaaatcccg cgcgctggcg attttaaaat cggccctcga
    37141 cagccagcag ggtgaaccat ggcaaacgat tcgtttaatt tctgaatgtt acccggaaga
    37201 cagcggcctg ttctccccgc tgttgctgaa tgtggtgaaa ttgaatcctg gcgaagcgat
    37261 gttcctgttc gctgaaacac cgcacgctta cctgcaaggc gtggcgctgg aagtgatggc
    37321 gaactccgat aacgtgctgc gtgcaggtct gacgcctaaa tacattgata ttccggaact
    37381 ggttgccaac gtgaagttcg aagccaaacc ggctaaccag ttgttgaccc agccggtgaa
    37441 acaaggtgca gaactggact tcccgattcc tgtggatgat tttgccttct cgttgcatga
    37501 ccttagtgat aaagaaacca ccatcagcca gcagagtgcc gccattttgt tctgcgtcga
    37561 aggcgatgca acgctgtgta aaggttctca gcagttacag cttaaaccgg gtgaatcagc
    37621 gtttattgcc gccaacgaat caccggtgac tgtcaaaggc cacggccgtt tagcgcgtgt
    37681 ttacaacaag ctgtaagagc ttactgaaaa aattaacatc tcttgctaag cttattaaag
    37741 gcttataaca ccttcaggcg gccagtccgc ctgatttcat tttatggata atcattatga
    37801 ataaatcgct ggtagcggta ggcgtcattg ttgcgctagg cgtagtctgg acaggcggcg
    37861 catggtatac aggcaagaag attgaaaccc atctcgaaga catggtcgcg caggcgaacg
    37921 cgcaactcaa actgaccgct cctgaatcca acctggaagt gagttatcaa aactatcatc
    37981 gcggcgtatt cagcagtcag ctgcaactgt tggtgaaacc cattgccggg aaagaaaatc
    38041 cgtggattaa aagcggtcag agcgtcatct tcaacgaatc ggttgatcat ggtcccttcc
    38101 cgcttgccca gcttaaaaaa ctgaacctga tcccgtcgat ggcatcaatt caaaccacgc
    38161 tggttaataa cgaagtaagc aaaccactgt ttgatatggc aaaaggtgaa acgccttttg
    38221 agattaactc ccgcattggt tacagcggag attccagttc cgacatttcg ctcaagccac
    38281 tgaattacga gcaaaaggat gaaaaagtcg cctttagcgg cggcgagttc cagttaaatg
    38341 cagacagaga cggcaaagct atctcccttt ccggggaggc gcaaagtggt cggatagacg
    38401 cggttaacga atacaaccag aaagtgcagt taacctttaa taatctgaaa accgacggtt
    38461 ccagcacgct ggcaagtttt ggtgagcgcg taggaaacca aaaactgtcg ctggaaaaaa
    38521 tgaccatttc agtggagggc aaagaactgg cactgctgga aggcatggag atcagcggta
    38581 aatcggatct ggtcaatgac ggtaaaacgg tcaatagcca actggattac tcgctaaaca
    38641 gcctgaaggt acagaatcag gatctgggca gtggcaagct gactttaaaa gtcggccaaa
    38701 ttgatggcga agcctggcat cagtttagcc agcaatataa cgcgcaaact caggcgctgc
    38761 tggcacagcc agaaattgcc aacaatcccg aactttatca ggagaaagtg acggaagcct
    38821 tctttagcgc tctgccgctg atgctgaaag gcgatccggt gattactatc gcgccgctaa
    38881 gctggaaaaa cagtcagggt gaaagtgcgc tgaatctgtc gctgttcctg aaagatccgg
    38941 caacgaccaa agaagcgccg caaacgctgg cgcaggaagt ggaccggtcg gttaaatctc
    39001 tggatgcgaa actgaccatt ccggtggata tggcaactga gttaatgact caggtagcga
    39061 agctggaagg ttatcaggaa gatcaagcga aaaaactggc gaaacagcaa gttgaaggtg
    39121 catcagcaat ggggcagatg ttccgtctga ccaccttgca ggacaatacc atcaccacca
    39181 gcctgcaata tgctaacggt cagataacgt taaacgggca gaaaatgcca ctggaagatt
    39241 tcgtgggtat gtttgcgatg ccggcattaa acgttccggc tgtacccgct attccgcagc
    39301 agtaattcac agccaaaacg ccggagattt ttctctccgg cgttttttgt gcacagaaaa
    39361 cccccagcta ggctgggggt tccggaaagc tttcagcttt gagccagtta ttaaaacccc
    39421 ttttgatttg ttaaaacacc ttgcggtctg gcaactgcaa gtgtcaaaca agaaatcaaa
    39481 agggggtccc aatggggaac gaaaagagct tagcgcacac ccgatggaac tgtaaatatc
    39541 acatagtatt tgcgccaaaa taccgaagac aggtgttcta cagagagaag cgtagagcaa
    39601 taggctgtat tttgagaaag ctgtgtgagt ggaaaagtgt acggattctg gaagctgaat
    39661 gctgtgcaga tcatatccat atgcttgtgg agatcccgcc caaaatgagc gtatcaggct
    39721 ttatgggata tctgaaaggg aaaagcagtc tgatgcctta cgagcagttt ggtgatttga
    39781 aattcaaata caggaacagg gagttctggt gcagagggta ttacgtcgat acggtgggta
    39841 agaacacggc gaagatacag gattacataa agcaccagct tgaagaggat aaaatgggag
    39901 agcagttatc gattccctat ccgggcagcc cgtttacggg ccgtaagtaa cgaagttaga
    39961 tgcaaatgtc agatcgtgtg cgcctgttag ggcgcggctg gtaagagagc cttataggcg
    40021 catttgaaaa acctccggct atgccggagg atatttattc ttacttcagc ataaagtcat
    40081 actgaatact cattttcaca tcccagtaat tcaggcgatc gcccagcgca ctgccatcgc
    40141 gcttttcccg ccctttatcg gcagcaagaa agaaattcat cccttgtaac ggcccttcgc
    40201 tgacccgata gttcataccc cctaagatac ccgcgccttc aatccgcaca tctcgtttac
    40261 tggattcata gccgcgagtc acaatcggtg caaatgtcag cgtaaattgc ggagttaaac
    40321 gatagttaac gcccaattgc caggactgca ttccttcacg gttacgatca agacttaatg
    40381 aaaagggata gccaatgtcg gtgtccacca cggcaccatt cattgcatgg cgcaaataac
    40441 caccactgcc aaatacgctt ccccacggcg caatccaggt caattgtcca ctaaccaacg
    40501 ccgtttcatt gggctggctg gtattacgac ttaaaccttc gagttgcgcg taaaagccga
    40561 gcaactcccc tttcaacgta taattcgggt cgagcggtat gcttttctcg agaatacttt
    40621 gtatctgatt ggaaacatct ttctgtaccg atgcccccag agaatagcga ctggtatcat
    40681 catgccaact ggcggctaaa aaagaacgtg cgcgatgttt atcgtatttt cccgtctggc
    40741 tgttatacac cccttcatca cgaataccgg ttgttgaatc ggtccataaa aatactcgtg
    40801 aacgccacat tccccacagg tcaatatttg gcgttaaagc ccaataacct aatgctcctt
    40861 cagaaactgt cggtaatgta cgttgcgaac tgccagccat taacggaata cgataaggta
    40921 agctaccata actataatcc gtcccgaagc gacctaattt aagcagacta ctttcactgg
    40981 gttttatttt tactgccccc agggtaaagc caaaactttt atcaccatca agataccacc
    41041 gggtgctcat atcagcacga gcacctaatt tataaacata atacgccccg ccttcaacgc
    41101 caataatatt ggaataataa ccactattga aatcgagcaa tcctccctgt acccatgcgt
    41161 aaatatctct ccccgcgcca gcactgggct tatcagccct gcgtaattca tttttcagtc
    41221 tgatgcgtag tcccgcttca tcgtcagcca gttgcgccgc gtaagcagag gtaaggccag
    41281 aggcagccgt caggcaaata acggccatgg ccactatttt tctc
//
LOCUS       DAZFJL010000021        41192 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFJL010000021 DAZFJL010000000
VERSION     DAZFJL010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.021"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(30..875)
                     /gene="araC"
                     /locus_tag="ACHJ8C_001170"
     CDS             complement(30..875)
                     /gene="araC"
                     /locus_tag="ACHJ8C_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO5551063.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAHEWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCE"
     gene            1214..2914
                     /gene="araB"
                     /locus_tag="ACHJ8C_001171"
     CDS             1214..2914
                     /gene="araB"
                     /locus_tag="ACHJ8C_001171"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO5551064.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQNGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQHAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKDQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPCSEQAQRFEQLYRRYQQWAMSAEKHYLPTSAPAQAAQAVPTL
                     "
     gene            2925..4427
                     /gene="araA"
                     /locus_tag="ACHJ8C_001172"
     CDS             2925..4427
                     /gene="araA"
                     /locus_tag="ACHJ8C_001172"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO5551065.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            4712..5407
                     /gene="araD"
                     /locus_tag="ACHJ8C_001173"
     CDS             4712..5407
                     /gene="araD"
                     /locus_tag="ACHJ8C_001173"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO5551066.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRGRGVLVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            5482..7833
                     /gene="polB"
                     /locus_tag="ACHJ8C_001174"
     CDS             5482..7833
                     /gene="polB"
                     /locus_tag="ACHJ8C_001174"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO5551067.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDSGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPLLLEKLNAWFATHDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNDVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRTDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKTLIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDTQL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENHKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            7998..10904
                     /gene="rapA"
                     /locus_tag="ACHJ8C_001175"
     CDS             7998..10904
                     /gene="rapA"
                     /locus_tag="ACHJ8C_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO5551068.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDETEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            10916..11575
                     /gene="rluA"
                     /locus_tag="ACHJ8C_001176"
     CDS             10916..11575
                     /gene="rluA"
                     /locus_tag="ACHJ8C_001176"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO5551069.1"
                     /translation="MGMENYNPPQDPWLIVLYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(11693..12508)
                     /gene="djlA"
                     /locus_tag="ACHJ8C_001177"
     CDS             complement(11693..12508)
                     /gene="djlA"
                     /locus_tag="ACHJ8C_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO5551070.1"
                     /translation="MQYWGKIIGVAVALIMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            12763..15117
                     /gene="lptD"
                     /locus_tag="ACHJ8C_001178"
     CDS             12763..15117
                     /gene="lptD"
                     /locus_tag="ACHJ8C_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO5551071.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYKDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTRLAESANRVMPQFKVDGRMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSKIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISDRWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            15170..16456
                     /gene="surA"
                     /locus_tag="ACHJ8C_001179"
     CDS             15170..16456
                     /gene="surA"
                     /locus_tag="ACHJ8C_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO5551072.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            16456..17445
                     /gene="pdxA"
                     /locus_tag="ACHJ8C_001180"
     CDS             16456..17445
                     /gene="pdxA"
                     /locus_tag="ACHJ8C_001180"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO5551073.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLIVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTVGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELREQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            17442..18263
                     /gene="rsmA"
                     /locus_tag="ACHJ8C_001181"
     CDS             17442..18263
                     /gene="rsmA"
                     /locus_tag="ACHJ8C_001181"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO5551074.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAA
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            18266..18643
                     /gene="apaG"
                     /locus_tag="ACHJ8C_001182"
     CDS             18266..18643
                     /gene="apaG"
                     /locus_tag="ACHJ8C_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO5551075.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            18650..19498
                     /gene="apaH"
                     /locus_tag="ACHJ8C_001183"
     CDS             18650..19498
                     /gene="apaH"
                     /locus_tag="ACHJ8C_001183"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO5551076.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYNIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLSE
                     GEAVAS"
     gene            complement(19495..19809)
                     /locus_tag="ACHJ8C_001184"
     CDS             complement(19495..19809)
                     /locus_tag="ACHJ8C_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HFO5551077.1"
                     /translation="MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(19812..20045)
                     /locus_tag="ACHJ8C_001185"
     CDS             complement(19812..20045)
                     /locus_tag="ACHJ8C_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO5551078.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(20131..20610)
                     /gene="folA"
                     /locus_tag="ACHJ8C_001186"
     CDS             complement(20131..20610)
                     /gene="folA"
                     /locus_tag="ACHJ8C_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO5551079.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(20802..22664)
                     /gene="kefC"
                     /locus_tag="ACHJ8C_001187"
     CDS             complement(20802..22664)
                     /gene="kefC"
                     /locus_tag="ACHJ8C_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO5551080.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFVLSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAIVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(22657..23187)
                     /gene="kefF"
                     /locus_tag="ACHJ8C_001188"
     CDS             complement(22657..23187)
                     /gene="kefF"
                     /locus_tag="ACHJ8C_001188"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO5551081.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(23295..24626)
                     /gene="yaaU"
                     /locus_tag="ACHJ8C_001189"
     CDS             complement(23295..24626)
                     /gene="yaaU"
                     /locus_tag="ACHJ8C_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5551082.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFEEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(24685..24972)
                     /gene="fixX"
                     /locus_tag="ACHJ8C_001190"
     CDS             complement(24685..24972)
                     /gene="fixX"
                     /locus_tag="ACHJ8C_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO5551083.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(24969..26255)
                     /gene="fixC"
                     /locus_tag="ACHJ8C_001191"
     CDS             complement(24969..26255)
                     /gene="fixC"
                     /locus_tag="ACHJ8C_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5551084.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(26306..27247)
                     /gene="fixB"
                     /locus_tag="ACHJ8C_001192"
     CDS             complement(26306..27247)
                     /gene="fixB"
                     /locus_tag="ACHJ8C_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO5551085.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(27262..28032)
                     /gene="fixA"
                     /locus_tag="ACHJ8C_001193"
     CDS             complement(27262..28032)
                     /gene="fixA"
                     /locus_tag="ACHJ8C_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO5551086.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEGQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAASQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            28505..30019
                     /gene="caiT"
                     /locus_tag="ACHJ8C_001194"
     CDS             28505..30019
                     /gene="caiT"
                     /locus_tag="ACHJ8C_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO5551087.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSVLVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            30050..31192
                     /gene="caiA"
                     /locus_tag="ACHJ8C_001195"
     CDS             30050..31192
                     /gene="caiA"
                     /locus_tag="ACHJ8C_001195"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO5551088.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            31321..32538
                     /gene="caiB"
                     /locus_tag="ACHJ8C_001196"
     CDS             31321..32538
                     /gene="caiB"
                     /locus_tag="ACHJ8C_001196"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO5551089.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIAECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            32611..34164
                     /gene="caiC"
                     /locus_tag="ACHJ8C_001197"
     CDS             32611..34164
                     /gene="caiC"
                     /locus_tag="ACHJ8C_001197"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO5551090.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREEST
                     WILQNSQACLLVTSAQFYPMYQQIQQKDATQLRHICLTDMALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSTGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDQQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFCFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            34273..35058
                     /gene="caiD"
                     /locus_tag="ACHJ8C_001198"
     CDS             34273..35058
                     /gene="caiD"
                     /locus_tag="ACHJ8C_001198"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO5551091.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            35064..35654
                     /gene="caiE"
                     /locus_tag="ACHJ8C_001199"
     CDS             35064..35654
                     /gene="caiE"
                     /locus_tag="ACHJ8C_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO5551092.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(35773..36168)
                     /gene="caiF"
                     /locus_tag="ACHJ8C_001200"
     CDS             complement(35773..36168)
                     /gene="caiF"
                     /locus_tag="ACHJ8C_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO5551093.1"
                     /translation="MCEGCVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(36429..39650)
                     /gene="carB"
                     /locus_tag="ACHJ8C_001201"
     CDS             complement(36429..39650)
                     /gene="carB"
                     /locus_tag="ACHJ8C_001201"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO5551094.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHSMEEALAVAA
                     EVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNADFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(39668..40816)
                     /gene="carA"
                     /locus_tag="ACHJ8C_001202"
     CDS             complement(39668..40816)
                     /gene="carA"
                     /locus_tag="ACHJ8C_001202"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO5551095.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEPYSWTQGSWTLTGGLPEAKKEDELPYHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTIKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIALIEQYRKTAK"
BASE COUNT         9858 a        11164 c        10781 g         9389 t
ORIGIN      
        1 gaaagtaaga gactccccct tctttcttat tattcacaac ctgccctaaa ctcgctcgga
       61 ctcgccccgg tgcatttttt aaatactcgc gagaaataga gttgatcgtc aaaaccgaca
      121 ttgcgaccga cggtggcgat aggcatccgg gtggtgctca aaagcagctt cgcctgactg
      181 atgcgctggt cctcgcgcca gcttaatacg ctaatcccta actgctggcg gaacaaatgc
      241 gacagacgcg acggcgacag gcagacatgc tgtgcgacgc tggcgatatc aaaattactg
      301 tctgccaggt gatcgctgat gtactgacaa gcctcgcgta cccgattatc catcggcgga
      361 tgaagcgact cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagatttatc
      421 gccagcaatt ccgaatagcg cccttcccct tgtccggcat taatgatttg cccaaacagg
      481 tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga aaccggtatt ggcaaatatc
      541 gacggccagt taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga
      601 taccattcgt gagcctccgg atgacgaccg tagtgatgaa tctctccagg cgggaacagc
      661 aaaatatcac ccggtcggca gacaaattct cgtccctgat ttttcaccac cccctgaccg
      721 cgaatggtga gattaagaat ataacccttc attcccagcg gtcgatcgat aaaaaaatcg
      781 agataaccgt tggcctcaat cggcgttaaa cccgccacca gatgggcgtt aaacgagtat
      841 cccggcagca ggggatcatt ttgcgcttca gccatacttt tcatactccc gccattcaga
      901 gaagaaacca attgtccata ttgcatcaga cattgccgtc actgcgtctt ttactggctc
      961 ttctcgctaa cccaaccggt aaccccgctt attaaaagca ttctgtaaca aagcgggacc
     1021 aaagccatga caaaaacgcg taacaaaagt gtctataatc acggcagaaa agtccacatt
     1081 gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat aagattagcg
     1141 gatccagcct gacgcttttt ttcgcaactc tctactgttt ctccataccc gtttttttgg
     1201 atggagtgaa acgatggcga ttgcaattgg cctcgatttt ggcagtgatt ctgtgcgagc
     1261 tttggcggtg gactgcgcca ccggtgaaga gatcgccacc agcgtagagt ggtatccccg
     1321 ttggcagaac gggcaatttt gtgatgcccc gaataaccag ttccgtcatc atccgcgtga
     1381 ctacattgag tcaatggaag cggcgctgaa aactgtgctt gcagagctta gcgtcgaaca
     1441 gcacgcagct gtggtcggga ttggcgttga cacaaccggc tcgacgcccg caccgattga
     1501 cgccgacggt aacgtgctgg cgctgcgccc ggagtttgcc gaaaacccga acgcgatgtt
     1561 cgtattgtgg aaagaccaca ccgcggttga agaagcggaa gagataaccc gcttatgtca
     1621 cacgccgggc aacgttgact actcccgcta tattggcggt atttattcca gcgaatggtt
     1681 ctgggcaaaa atcctgcatg tgactcgcca ggacagcgcc gtggcgcaat ctgccgcatc
     1741 gtggattgag ctgtgcgact gggtaccagc tctgctttcc ggtaccaccc gcccgcagga
     1801 tattcgtcgc ggacgttgca gcgccggaca taagtcgcta tggcatgaaa gctggggcgg
     1861 cctgccgcca gccagtttct ttgacgaact ggacccgatc ctcaatcgcc atttgccttc
     1921 cccgctgttc actgatacct ggactgccga tattccggtg ggcacgttat gtccggaatg
     1981 ggcgcagcgt ctcggcctgc ctgaaagcgt ggtgatttcc ggcggcgcgt ttgactgcca
     2041 tatgggcgca gttggtgcag gcgcacagcc taacgcactg gtaaaagtta tcggtacttc
     2101 gacctgcgac attctgattg ccgacaaaca gagcgttggc gagcgggcag tgaaaggtat
     2161 ttgcggtcag gttgatggca gcgtggtgcc tggatttatc ggtctggaag caggccaatc
     2221 ggcgtttggc gatatctacg cctggttcgg tcgcgtactc ggctggccgc tggaacagct
     2281 tgccgcccag catccggaac tgaaagatca aatcaacgcc agccagaaac aactgcttcc
     2341 ggcgctgacc gaagcatggg ctaaaaaccc gtctctggat cacctgccgg tagtactcga
     2401 ctggtttaac ggtcgtcgca cgccaaacgc taaccaacgc ctgaaagggg tgattaccga
     2461 tctgaacctc gctaccgacg ctccgctact gttcggcggt ttaattgccg ccaccgcctt
     2521 tggcgcacgc gcaattatgg agtgcttcac cgatcagggg atcgccgtca ataacgtgat
     2581 ggcactgggc ggcatcgcgc gcaaaaacca ggtcattatg caggcctgct gcgacgtgct
     2641 gaatcgcccg ctgcaaattg ttgcctctga ccagtgctgt gcgctcggtg cggcgatttt
     2701 cgctgccgtc gccgcgaaag tgcacgcaga catcccatca gcccagcaaa aaatggccag
     2761 tgcggtagag aaaaccctgc aaccgtgcag cgaacaggca caacgctttg aacagcttta
     2821 tcgccgctat cagcaatggg cgatgagcgc cgaaaaacac tatcttccaa cttccgcccc
     2881 ggcacaggct gcccaggccg ttccgactct ataaggacac gacaatgacg atttttgata
     2941 attatgaagt gtggtttgta attggcagcc aacatctgta tggcccggaa accctgcgtc
     3001 aggtcaccca acatgccgag cacgtcgtta atgcgctgaa tacggaagcg aaactgccct
     3061 gcaaactggt gctgaaaccg ctgggcacca cgccggatga aatcaccgct atttgccgtg
     3121 acgcgaatta cgacgatcgt tgcgctggcc tggtagtgtg gctgcacact ttctccccgg
     3181 ccaaaatgtg gatcaacggc ctgactatgc tcaacaaacc gttgctgcaa ttccacaccc
     3241 agtttaacgc ggcgctgccg tgggacagta tcgatatgga ctttatgaac ctgaaccaga
     3301 ctgcacatgg cggtcgtgag ttcggcttca tcggcgcgcg tatgcgtcag caacatgccg
     3361 tggttaccgg tcactggcag gataaacaag cacatgagcg tatcggctcc tggatgcgtc
     3421 aggcggtatc taaacaggat acccgtcatc tgaaagtctg ccgttttggc gataacatgc
     3481 gtgaagtggc ggtcaccgat ggtgataaag ttgccgcaca gatcaagttc ggtttctccg
     3541 tcaatacctg ggcggttggc gatctggtgc aggtggtgaa ctccatcagc gacggcgatg
     3601 ttaacgcgct ggtcgatgag tacgaaagct gctacaccat gacgcctgcc acacaaatcc
     3661 acggcgaaaa acgccagaac gtgctggaag cggcgcgtat tgagctgggg atgaagcgtt
     3721 tcctggaaca aggtggcttc cacgcgttca ccaccacctt tgaagatttg cacggtctga
     3781 aacagcttcc tggtctggcc gtacagcgtc tgatgcagca gggttacggc tttgcgggcg
     3841 aaggcgactg gaaaactgcc gccctgcttc gcatcatgaa ggtgatgtca accggtctgc
     3901 agggcggcac ctcctttatg gaggactaca cctatcactt cgagaaaggc aatgacctgg
     3961 tgctcggctc ccatatgctg gaagtctgcc cgtcgatcgc cgcagaagag aaaccgatcc
     4021 tcgacgttca gcatctcggt attggtggta aggacgatcc tgcccgcctg attttcaata
     4081 cccaaaccgg cccagcgatt gtcgccagct tgattgatct cggcgatcgt taccgtctac
     4141 tggttaactg catcgacacg gtgaaaacac cgcactccct gccgaaactg ccggtggcga
     4201 atgcgctgtg gaaagcgcaa ccggatctgc caactgcttc cgaagcgtgg atcctcgctg
     4261 gtggcgcgca ccataccgtt ttcagccatg cgctgaacct caacgatatg cgccagttcg
     4321 ccgagatgca cgacattgaa atcacggtga ttgataacga cacccgcctg ccagcgttta
     4381 aagacgcgct gcgctggaac gaagtgtatt acggatttcg tcgctaagta gccgaatcag
     4441 gtatgtaacg cctgatgcga cgctgacgcg tcttatcagg cctacacgct gcgattttgt
     4501 aggccggata agcaaagcgc atccggcatt caccgcctga tgcggcgctg gcgcgtctta
     4561 tcaggcctac gcgttccgga tttgtaggcc ggataagcaa agcgcatccg gcattcaccg
     4621 cctgatgcga cgccgacgcg tcttatcagg cctacacgct gcgattttgt aggccggata
     4681 agcaaagcgc atccggcacg aaggagtcaa catgttagaa gatctcaaac gccaggtatt
     4741 agaagccaac ctggcgctgc caaaacacaa cctggtcacg ctcacctggg gcaatgtcag
     4801 cgccgttgat cgcgggcgcg gcgtcctggt gatcaaacct tccggcgtcg actacagcat
     4861 catgaccgcc gacgatatgg tcgtggttag catcgaaacc ggtgaagtgg ttgaaggaac
     4921 gaaaaagccc tcctccgaca cgccaactca ccggctgctc tatcaggcat tcccgtccat
     4981 tggcggcatt gtgcacacac actcgcgcca cgccaccatc tgggcgcagg cgggtcagtc
     5041 gattccagca accggtacca cccacgccga ctatttctac ggcaccattc cctgcacccg
     5101 caaaatgacc gacgcagaaa tcaacggtga atatgagtgg gaaaccggta acgtcatcgt
     5161 agaaaccttc gaaaaacagg gtatcgatgc ggcgcaaatg cccggcgtgc tggtccattc
     5221 tcacggccca tttgcatggg gcaaaaatgc cgaagatgca gtgcataacg ccatcgtgct
     5281 ggaagaggtc gcttatatgg ggatattctg ccgtcagtta gcgccgcagt taccggatat
     5341 gcagcaaacg ctgctggata aacactatct gcgtaagcat ggtgcgaagg catattacgg
     5401 gcagtaatga ctgtataaaa ccacagccaa tcaaacgaaa ccaggctata atcaagcctg
     5461 gttttttatt ggattttcag cgtggcgcag gcaggtttta tcttaacccg acactggcgg
     5521 gacaccccac aaggtacaga agtctccttc tggctggcga cggacagcgg gccgttgcag
     5581 gttacgcttg caccgcaaga gtccgtggcg tttattcccg ccgatcaggt tccccgcgcc
     5641 cagcatattt tgcagggtga gcaaggcttt cgcctgacgc cgctggcgtt aaaggatttt
     5701 catcgccagc cggtgtatgg cctttactgt cgcgcccatc gccagttgat gaattacgaa
     5761 aagcgcctgc gtgaaggtgg cgttaccgtc tacgaggccg atgtgcgccc gccagaacgc
     5821 tatctgatgg agcggtttat cacctcgcca gtgtgggtcg agggtgatat gcgcaacggc
     5881 gctatcgtta atgcccgtct gaaaccgcat cccgactatc gtccgccgct caagtgggtt
     5941 tctattgata ttgaaaccac ccgccacggt gagctgtact gcatcggcct ggaaggttgc
     6001 gggcagcgta tcgtttatat gctggggcca gagaatggcg acgcctccgc gctcgatttt
     6061 gaactggaat acgtcgccag ccgcccgctg ctactggaaa aacttaacgc ctggtttgcg
     6121 actcatgatc ctgatgtgat catcggttgg aacgtggtgc agttcgatct gcgaatgctg
     6181 caaaaacatg ccgagcgtta ccgtattccg ctgcgtctgg ggcgtgataa cagcgagctg
     6241 gagtggcgcg agcacggctt taaaaacgac gtcttttttg ctcaggccaa agggcgactg
     6301 attatcgacg gtatcgaggc gctgaaatcc gcgttctgga atttctcttc attctcgctg
     6361 gaaaccgtcg ctcaggagtt attaggcgaa ggaaaatcta tcgataaccc gtgggatcga
     6421 acggacgaaa ttgaccgccg tttcgccgaa gataagcctg cgctggcaac ttataacctg
     6481 aaagattgcg agctggtgac gcagatcttc cacaaaactg aaatcatgcc gtttttgctc
     6541 gaacgggcga cggtgaacgg cctgccggta gatcgacacg gcggttcggt ggcagcgttt
     6601 ggtcatctct attttccccg tatgcatcgc gctggttatg tcgcgcctaa tctcggcgaa
     6661 gtgccgccac acgccagccc tggcggttat gtgatggatt cacggcctgg actgtatgat
     6721 tcggtgctgg tgctggacta caaaagcctg tacccgtcga tcatccgcac ctttctgatt
     6781 gatcccgtcg ggctggtgga aggcatggcg cagcctgatc cagagcacag taccgaaggt
     6841 tttctcgatg cctggttctc acgggagaaa cattgcctgc cggagattgt gactaacatc
     6901 tggcacgggc gcgatgaagc caaacgccag ggtaataagc cgctttcgca ggcgctgaag
     6961 atcattatga atgcctttta tggtgtgctc ggcaccaccg cctgccgttt cttcgatcca
     7021 cgcctggcat cgtcgatcac catgcgtggt catcagatca tgcggcaaac caaaacgttg
     7081 attgaagcgc agggctatga tgttatctac ggcgataccg actcaacgtt tgtctggctg
     7141 aaaggtgcac attcggaaga agaagcagcg aaaatcggtc gtgcactggt gcagcacgtt
     7201 aacgcctggt gggcggaaac gctgcaaaaa caacggctga ccagcgcact ggaactggag
     7261 tatgaaaccc atttctgccg ttttctgatg ccaaccattc gcggggccga taccggcagc
     7321 aaaaagcgct acgccggact gattcaggag ggcgacaaac agcggatggt gtttaaaggg
     7381 ctggaaaccg tgcgcaccga ctggacgccg ctggcccagc agtttcagca ggagctttac
     7441 ctgcgcatct tccgcaacga gccatatcag gaatacgtgc gcgaaaccat cgacaagctg
     7501 atggcgggcg aactggacac gcagctggtt taccgtaaac gccttcgccg tccgctgagc
     7561 gaatatcagc gtaatgttcc gcctcatgta cgcgccgctc gccttgccga tgaagaaaac
     7621 cataagcgcg gtcgcccctt gcaatatcag aaccgcggca ccattaagta cgtatggacc
     7681 accaacggcc cggagccgct ggactaccaa cgttcaccac tggattacga acactatctg
     7741 acccgccagc tacaacccgt ggcggaggga atactccctt ttattgagga taattttgct
     7801 acacttatga ccgggcaact tgggctattt tgagcaaaaa aaagagttcg ccagatacca
     7861 ttttgatgcg tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac
     7921 ctgaataaca ccactacctc ataagcacgg tagcgggtgg ttattgcctg caattaaaga
     7981 tatagagccg aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa
     8041 gcgaattggg acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc
     8101 catctactgg tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt
     8161 tcaaccctgg tgataccatt accagccatg acggctggca gatgcaagtc gaagaagtaa
     8221 aagaagaaaa tggcttgctg acctatatcg gtactcgcct tgatactgaa gagtccggcg
     8281 tagccctgcg tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc
     8341 tgtttgccgg gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt
     8401 attccagcga acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga
     8461 tcccgcacca gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc
     8521 tggctgacga agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac
     8581 tgctctctgg cgctgctgaa cgtgtgctga ttatcgtccc ggaaacctta cagcatcagt
     8641 ggctggtaga aatgctgcgc cgtttcaacc tgcgctttgc tctgtttgat gatgagcgtt
     8701 atgccgaagc tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct
     8761 cgctggattt tgcccgtcgt agcaaacagc gtctggaaca tctctgtgaa gccgagtggg
     8821 acctgctggt ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccgagccgtg
     8881 aatatcaggc cattgaacaa ctggcagagc acgtgcccgg cgttctgctg ctgaccgcaa
     8941 ccccggaaca actggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc
     9001 gtttccacga ttttgcgcaa ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg
     9061 ccgttgccat gctgctggca ggtaacaaac tcagcaatga cgaactgaac atgctcggtg
     9121 agatgatcgg cgagcaggat atcgagccgc tgttgcaagc agcaaacagc gacagcgaag
     9181 atgcccagag cgcccgtcag gaactggttt cgatgctgat ggatcgccac ggcaccagcc
     9241 gcgtgctgtt ccgtaacacc cgtaacggtg tgaaaggctt cccgaaacgc gagctgcata
     9301 ctattaagct gccactgccg acgcagtatc agaccgctat taaagtctcc ggcattatgg
     9361 gcgcacgtaa aagtgcggaa gatcgcgctc gcgatatgct ctacccggag cgtatttatc
     9421 aggaatttga aggcgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga
     9481 tgggctacct gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagccgcta
     9541 ccgcgctgca actggagcag gtactgcgcg agcgtgaagg aattcgcgct gcggtgttcc
     9601 acgaaggcat gtcgattatc gaacgtgacc gcgctgccgc ctggtttgct gaagaagaca
     9661 ccggtgcgca ggtactgctg tgctcggaaa tcggttctga aggacgtaac ttccagtttg
     9721 ccagccacat ggtaatgttt gacctgccat tcaacccgga tctgctggag cagcgtattg
     9781 gtcgtctgga tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaaa
     9841 aaaccgctca gtcggtgctg gtgcgctggt atcacgaagg tctggatgca ttcgagcaca
     9901 cctgcccgac cggacgcact atttacgata gcgtgtacaa cgatttgatt aactatctgg
     9961 cctcgccgga tgagaccgaa ggctttgacg atctgatcaa aaactgccgc gagcagcatg
    10021 aagcgctgaa agcacaactg gaacagggcc gtgaccgcct gctggagatc cactccaacg
    10081 gtggcgaaaa agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc
    10141 tgatcgcctt cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg
    10201 acaacatgat cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt
    10261 cggaagatgg catcaccatc accttcgacc gtgaagtggc gctggcgcgt gaagatgcac
    10321 agtttattac ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg
    10381 ataccggtag cagcacgatt tcactgttga aaaacaaagc gttgccggta ggtacgctgt
    10441 tggtggaact gatttatgtg gttgaagccc aggctccgaa gcagttgcag ctcaaccgct
    10501 tcctgccacc gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc
    10561 aggtagagtt tgaaaccttt aaccgccagc ttaacgcggt taaccgtcac accggcagca
    10621 agctggttaa cgccgtgcag caggatgttc acgctatcct tcagctgggg gaagcgcaga
    10681 tcgagaaatc tgcccgtgca ttgattgatg cagcgcgtaa cgaagccgac gaaaaactgt
    10741 ctgccgagct gtctcgtctg gaagcgctgc gtgcagttaa cccgaacatt cgtgacgacg
    10801 aactgaccgc cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct
    10861 ggcgtctgga tgccctgcgt ttgatcgttg tgacgcatca gtaacggagc cgaagatggg
    10921 gatggaaaac tacaatccgc cgcaggaccc ctggctgatt gtcctgtatc aggatgacca
    10981 tattatggtg gtcaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca
    11041 caaagacagc gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg
    11101 tctggatatg gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga
    11161 gttaaaacgc cagttccgcg agcgcgagcc gaaaaaacag tatgtggccc gcgtctgggg
    11221 gcatccatcc cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg
    11281 cccgaaacag aaagtctgtt acgaaacagg taaacctgcg cagacggagt atgaagtggt
    11341 ggaatatgcg gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca
    11401 tcagctgcgt gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc
    11461 atcaccagaa gcgagagcaa tggcaccacg tttgttgcta catgcagaga tgctgacgat
    11521 tacccatccg gcgtatggca atagtatgac gtttaaagca ccagcggatt tttaatagtc
    11581 acgctggcga agttcggtag gccggagaag gcgttcacgc cgcatccggc aattttgcgc
    11641 cgattgcctg atgcgacgcg ggccgcgtct tatcaggcct acacgttgaa acttatttaa
    11701 accctttctg ctgctttatc agctcatagg cctgctgaat ttcctgcgct ttctgcttcg
    11761 ccatctccat catctctggc ggcaaacctt tcgccaccaa cttatcggga tggtgttcac
    11821 tcatcagctt acggtaggca cgtttgatgg tggtcgcatc atccgtcggc ttcacgccca
    11881 gcacattaca ggcatcttcc agcgtcgggc cacgctgcgc ttgctgccag ttaccaccgc
    11941 cagattgctg ctgataaccg ccgccaaact gtgcaccgcc ctgcatcatg cgcaaaaact
    12001 ggtcaaactg agcgcgggag atccctaatt cttcagcaat gacatacagc accgcccgtt
    12061 cattcgggtg cagtgaacca tcagcaaacg ccgcctgaat ctggatctcc agaaacatac
    12121 gaattaagtc aaaacgacca aagcagacac tgcgaaactg gcgcatcttt tcgcgcagcg
    12181 ggtaattgtc tgattttccc acccggaacg cattttgcgc cgcagtacgg gaagcgccat
    12241 gaaggttcat tcggtccatc aactggctgg cgatatgaat atcagcttcc gtgacgcgac
    12301 ctttggattt ggttaaatgc cccatcactt caaaagtggt ggcaaaaaac agcgcctgac
    12361 gctcacgctg gttggcgaac cacgccattt tacggctacg ggctttatca aacatatggc
    12421 caattaacag gcccaatact acgccccaaa agccgccgcc cattattaag gccacggcca
    12481 cgccaatgat ttttccccaa tactgcatat attccccaaa tcgacacacg gatatcaggg
    12541 ctatctccca caatataaag gcgcttttac ccatttctgg ctgcggtcta ttgtgagaca
    12601 tcgcctataa tttgcattat catacctgtc attcaatgcc gtgcctaaca ccacagacgc
    12661 tattcggaca ggattaacac tagcgtagag atgacgagta cgttagtctc tgaccgtttg
    12721 tcacgcgcaa cgttaccgat gatggaacaa taaaatcaac gtatgaaaaa acgtatcccc
    12781 actctcctgg ccaccatgat tgccaccgcc ctttatagtc aacagggact ggcagccgac
    12841 ctcgcctcac agtgcatgtt gggcgtgcca agctatgacc gtcctctggt acagggcgat
    12901 accaatgact tacccgtgac tatcaatgct gaccacgcga aaggggacta cccggatgac
    12961 gccgtgttta ctggcagcgt ggatatcatg cagggtaaca gccgtctgca ggccgacgaa
    13021 gtgcagctcc atcaaaaaga ggcaccagga caaccggagc cggtacgtac cgttgatgcg
    13081 ctcggtaatg tccattacga cgataaccag gtgatcctca aagggccgaa aggctgggcg
    13141 aatctgaaca ccaaagatac caacgtctgg gagggtgatt accagatggt gggtcgccag
    13201 ggtcgcggta aagcggacct gatgaaacaa cgtggcgaaa accgctatac cattctggat
    13261 aacggtagct ttacctcctg tctgccgggt tctgacacct ggagcgtggt aggtagcgaa
    13321 attattcatg accgcgaaga acaagttgcg gagatctgga acgcccgctt taaggtgggt
    13381 ccggtaccga tcttttatag cccgtatttg cagttgccgg tgggtgacaa acgtcgctct
    13441 ggtttcttga tcccgaacgc caaatacacc accaccaact actttgagtt ctacctgcca
    13501 tattactgga acatcgcgcc aaatatggat gccaccatca cgccgcatta tatgcatcgt
    13561 cgtggcaaca tcatgtggga gaacgaattc cgctacctct cccaggcggg cgctggcttg
    13621 atggaactgg actatctgcc ttcagataaa gtctataaag atgaacaccc gaacgatgac
    13681 agttcacgtc gttggttgtt ctactggaac cactccgggg tcatggatca ggtgtggcgt
    13741 ttcaacgtcg actacaccaa ggtcagcgat cctagctact tcaatgactt cgataacaaa
    13801 tacggttcca gtaccgacgg ctacgcaacg caaaaattca gcgttggcta tgcggtgcaa
    13861 aacttcaatg ccaccgtttc aaccaagcag ttccaggtct tcagcgagca gaacaccagt
    13921 agctactcgg cagagccgca gttagacgtt aactactatc aaaacgatgt cggtcctttc
    13981 gatacgcgaa tttacggcca ggcggtgcac tttgttaaca ccagagatga catgccagag
    14041 gcgactcgtg ttcacctgga accgaccatc aatctgccgc tctctaataa ctggggcagc
    14101 atcaatactg aagcgaagtt gctggcaacc cattatcagc aaaccaatct tgactggtat
    14161 aactccagaa acacgaccag gctggcagag tccgctaacc gtgtaatgcc gcagttcaag
    14221 gtagacggta gaatggtctt cgagcgtgat atggaaatgc tggcgccggg ctatacccag
    14281 acactggaac cacgcgcgca gtatctgtat gtgccatatc gcgatcagag caaaatctat
    14341 aactacgact cttcgctgct gcaatctgac tactccggcc tgttccggga ccggacttac
    14401 ggcggtcttg accgtattgc ctccgctaac caggtgacga ccggtgtcac atctcgcata
    14461 tatgatgatg ctgccgttga acgttttaat atttccgttg gtcaaatcta ctatttcacg
    14521 gagtctcgca ctggcgatga caacataaca tgggagaatg acgacaaaac gggctcactg
    14581 gtgtgggcag gcgatactta ctggcgcatc tctgatcgtt ggggattgcg tggcgggatt
    14641 cagtacgata cacgtctgga taacgtagcg accagtaact ccagcattga ataccgtcgg
    14701 gatgaagacc gtctggtaca gctgaattac cgttacgcca gcccggaata tattcaggca
    14761 acgctgccta agtactattc cactgctgag caatataaga atggtatttc gcaggtaggt
    14821 gccgtcgcca gctggccaat tgccgatcgt tggtccattg ttggggccta ctactacgac
    14881 accaatgcga acaagcaagc cgactctatg ttaggtgtgc aatacagctc ctgctgctat
    14941 gcgattcgcg tcggttacga gcggaagctg aacggttggg ataacgataa acaacatgcg
    15001 gtatatgaca acgcaatcgg ctttaacatc gaacttcgcg gcctgagctc caactacggt
    15061 ctgggtacgc aagagatgct gcgttcgaac attctgccgt atcaaaacac tttgtgattt
    15121 gttgatttac cacgtaatcc gcagtgcggt taattgaaat ggaaaaagta tgaagaactg
    15181 gaaaacgctg cttctcggta tcgccatgat cgcgaatacc agtttcgctg ccccccaggt
    15241 agtcgataaa gtcgcagccg tcgtcaataa cggcgtcgtg ctggaaagcg acgttgatgg
    15301 attaatgcag tcggtaaaac tgaacgctgc tcaggcaagg caacaacttc ctgatgacgc
    15361 gacgctgcgc caccaaatca tggagcgttt gatcatggat caaatcatcc tgcagatggg
    15421 gcagaaaatg ggagtgaaaa tctccgatga gcagctggat caggcgattg ctaacatcgc
    15481 gaaacagaac aacatgacgc tggatcagat gcgcagccgt ctggcttacg atggtctgaa
    15541 ctacaacacc tatcgtaacc agatccgcaa agagatgatt atctctgaag tgcgtaacaa
    15601 cgaggtgcgt cgtcgcatca ccatcctgcc gcaggaagtc gaatccctgg cgcagcaagt
    15661 gggtaaccaa aacgacgcca gcactgagct gaacctgagc cacatcctga tcccactgcc
    15721 ggaaaacccg acctctgatc aggtgaacga agcggaaagc caggcgcgcg cgattgtcga
    15781 tcaggcgcgt aacggcgctg atttcggtaa gctggcgatt gctcattctg ccgaccagca
    15841 ggcgctgaac ggcggacaga tgggctgggg ccgtattcag gagttgccgg ggatcttcgc
    15901 ccaggcatta agcaccgcga agaaaggcga cattgttggc ccgattcgtt ccggcgttgg
    15961 cttccatatt ctgaaagtta acgacctgcg cggcgaaagc aaaaatatct cggtgaccga
    16021 agttcatgct cgccatattc tgctgaaacc gtcgccgatc atgactgacg aacaggcccg
    16081 cgtgaaactg gaacagattg ctgccgatat caagagtggt aaaacgactt ttgctgccgc
    16141 agcgaaagag ttctctcagg atccaggctc tgctaaccag ggcggtgatc tcggctgggc
    16201 tacaccagat attttcgatc cggccttccg tgacgccctg acccgcctga acaaaggtca
    16261 aatgagtgca ccggttcact cttcattcgg ctggcattta atcgaactgc tggatacccg
    16321 taatgtcgat aaaaccgacg cggcacagaa agatcgtgca taccgcatgc tgatgaaccg
    16381 taagttctcg gaagaagcag caagctggat gcaggaacaa cgtgccagcg cctacgttaa
    16441 aatcctgagc aactaatggt taaaacccaa cgtgttgtga tcactcccgg cgagcccgcc
    16501 gggattggcc cggacttaat tgtccagctt gcacagcgtg agtggccggt cgaactggtt
    16561 gtttgtgccg atgccactct ccttaccgac cgggcagcga tgctcggttt gccgctcacc
    16621 ctccgccctt attcccccaa ctcccctgca caaccgcaaa ctgcgggcac attaacgcta
    16681 cttcctgtcg cactacgtga atctgtcact gtggggcagt tagcagttga aaatgggcac
    16741 tatgtggtgg aaacgctggc gcgagcgtgt gatggctgtc tgaacggtga atttgctgcg
    16801 ctgatcacag gccccgtgca taaaggcgtc attaacgacg caggcattcc gtttaccggt
    16861 cataccgagt ttttcgaaga gcgttcgcag gcgaaaaaag tggtgatgat gctggcaacc
    16921 gaagaacttc gcgtggcgct ggcaacgacg catttaccgc tgcgcgatat cgcagatgct
    16981 atcaccccag cccttttgca cgaagtgatt gctattttgc atcacgattt gcggaccaaa
    17041 tttggtattg ccgaaccgcg cattctggtc tgcgggctga atccgcacgc gggcgaaggc
    17101 ggtcatatgg gtacggaaga gatagacacc attattccgg tactcaacga gctgcgggaa
    17161 caggggatga aactcaacgg gccgctgcct gccgataccc tgtttcagcc gaaatatctc
    17221 gataacgccg acgccgtgct ggcgatgtac cacgatcagg gtcttcccgt gctaaaatac
    17281 cagggcttcg ggcgcggtgt gaacattacg ctgggcctgc cctttattcg cacatcagtg
    17341 gaccacggca ccgcgcttga actggcggga cgtggtaaag ccgatgtcgg cagttttatt
    17401 acggcgctta atctcgccat caaaatgatt gttaacaccc aatgaataat cgagtccacc
    17461 agggccactt agcccgtaaa cgcttcgggc aaaactttct caacgatcag ttcgtgatcg
    17521 acagcattgt ttctgccatt aacccgcaga agggccaggc gatggtcgaa atcggccccg
    17581 gtctggcggc attgaccgaa ccggtcggcg aacgtctgga ccagctgacg gtcattgaac
    17641 ttgaccgcga tctggcggca cgtctgcaaa cgcatccatt cttaggcccg aaactgacga
    17701 tttatcagca ggatgcgatg acctttaact ttggcgaact ggccgcgaaa atgggccagc
    17761 cgctgcgtgt tttcggcaac ctgccttata acatctccac gccgttgatg ttccatctgt
    17821 ttagctatac tgatgccatt gccgacatgc actttatgtt gcaaaaagaa gtggtgaatc
    17881 gtctggttgc aggaccgaac agcaaggcgt atggtcgatt aagcgtcatg gcgcaatact
    17941 attgcaacgt gatcccggtg ctggaagtac cgccgtcagc ctttactcca ccacccaaag
    18001 tggattccgc cgtcgtgcgc ctggttcctc atgcaacgat gcctcacccg gttaaagatg
    18061 ttcgcgtgtt gagccgcatc accaccgaag cctttaacca gcgtcgtaaa accattcgta
    18121 acagcctcgg caacctgttt agcgtcgagg tgttaacggg aatggggatc gacccggcga
    18181 tgcgagcgga aaatatctct gtcgcgcaat attgccagat ggcgaactat ctggcggaga
    18241 acgcgccttt gcaggagagt taacgatgat caattcgccc cgagtgtgta ttcaggttca
    18301 aagcgtctac attgaggcac aatcttcacc tgataatgaa cgttacgttt ttgcttatac
    18361 cgtaaccata cgcaatctgg ggcgagcgcc agtgcagttg ttggggcgtt actggctgat
    18421 caccaatggc aatggccgtg aaaccgaagt ccagggcgaa ggagtggttg gcgtccagcc
    18481 acttatcgcg cctggcgaag agtaccagta caccagcggt gcaatcattg aaaccccgct
    18541 gggcaccatg cagggtcact acgaaatgat cgatgaaaat ggcgtccctt tcagcatcga
    18601 cattcccgta ttccgactcg ccgttcccac actcattcat taaaagaata tggcgacata
    18661 ccttattggc gacgttcatg gttgttacga tgaactgatc gcattgctgc ataaagtaga
    18721 atttacccct gggaaagata ccctctggct gacgggcgat ctggtcgcgc gcggcccggg
    18781 ttcgctggat gttctgcgct atgtgaaatc cttaggcgac agcgtacgtc tggtgctggg
    18841 taatcacgat ctgcatctgc tggcggtatt tgccgggatc agccgcaata aaccgaaaga
    18901 tcgcctgaca ccgctgctgg aagcgccgga tgccgacgag ctgcttaact ggctgcgtcg
    18961 ccagcctttg ctgcaaatcg acgaagagaa aaagttggtg atggcccacg ccgggatcac
    19021 gccgcagtgg gatctgcaga ccgccaaaga gtgcgcgcgc gatgtagaag cggtgctgtc
    19081 gagtgactcc tatcccttct tccttgatgc catgtacggc gatatgccaa ataactggtc
    19141 accggaattg cgggggctgg gaagactgcg ttttatcacc aacgccttta cccgtatgcg
    19201 tttttgcttc ccgaacggtc aactggatat gtacagcaaa gaatcgccgg aagaggcccc
    19261 tgccccactg aaaccgtggt ttgcgattcc tggccccgtc gctgaagagt acaacatcgc
    19321 ctttggtcac tgggcatcgc tggaaggcaa aggtacgccg gaaggtattt acgcgctgga
    19381 taccggctgc tgctggggtg gtacattaac ctgcctgcgc tgggaagata aacagtattt
    19441 tgtccagccg tcgaaccggc ataaggattt gagtgaggga gaggcggtgg cgtcttaaat
    19501 cccgtcgagc ataaaatcta atgctccttt aatcaacgtt cggtgcgcgc tggcatcgca
    19561 aaatttcgtt cccagcgcgt taactggaac agttgcagtc tcatgcgtca tgagcacata
    19621 ttctttgccg tctaccagta aaaggattgg gttaagccgt tctggtgggc gaatacggct
    19681 aaatcgttca ataggtgtta atggaataac gatacggcga ttaatctcac caataatgtc
    19741 gctggtaaca tcaataacaa agggaaacgc ggcgttcctg ccgcgactgc ggtataccgt
    19801 aaattgcatc gctagaagct ccggtactca tcagagaaac atccggtttc atcagccaat
    19861 tgatttaacg cagcgatagc ttctgcgttc tcttcacgcc aacgtgttgc tgcatgtttt
    19921 ttaagttcag cattgagcgc aaccgtaagg gtggcgctca tatttaagcc tgcatcacga
    19981 gcgcgattga ctaactcacg gtcgacggtg acggtcacac tttgtgtggt acgttttgca
    20041 gtcatgatct cgtgctcctg tacaatttat acgtctagta tacacggata tctgcacgta
    20101 tcagtgatgc cggaattcta atatacaaaa ttaccgccgc tccagaatct caaagcaata
    20161 gctgtgagag ttctgcgcat cggcatcgtg gaattcactg aataccgact cccagtcatc
    20221 cggctcgtaa tccgggaaat gggtgtcgcc ttccacttcc gcgtcgatat gcgtcagata
    20281 cagtttttgc gctttcggca ggaactgctc ataaacgcga ccgccgccaa tcaccatgat
    20341 ttctggtacg tcaccacacg ccgcaatggc ttcatccacc gacttcaccc acgttacgcg
    20401 atcgtccgta cccggttgac tgctgaggat aatatttttg cgtcctggca acggacgacc
    20461 gatggattcc caggtatggc ggcccataat cacgggttta tttaaagtgt tgcgtttaaa
    20521 ccaggcgaga tcggcaggca ggttccacgg catggcgttt tccatgccga taacgcgatc
    20581 taccgctaac gccgcaatca gactgatcat tgagatttcc cgataaaaaa aattgtcgcc
    20641 actatacgta aagcgtaaac cgtcgtcgac tggtgcgagg atgatgttga ggaaaatttt
    20701 atattctgct ggcgagtcca cgctctctcc ctggactcgc cgcattacaa tgaaacaaaa
    20761 acaaacagtt agctgtaaag cgtgatttac gtcactcttt attaagatga gggcttcgtt
    20821 tccggttcat ccgccatatt gccagtatgt ttcccttctt cggttccctg ccagccatgt
    20881 cgttgaatta atgacagatg ttcacggtct tcggtaatga tctcacttaa catcgcgctg
    20941 gtgcgtttat agaccgccgc acgggctttg gtgtcgttct caacaattgc catctcttcc
    21001 accatctgaa tattaaagcg gcggaacaca tcggcgcgtt ctcgcgcttc atacggcccc
    21061 agacctaaac tttccagtgc cagacgcccg gttttcagcg caccttcgaa ggtttcacgc
    21121 tccggctttt caacgcctgc ctgacgcaaa cgaatgtagt ggtcgacatc gcgggcgcgg
    21181 gcaataatct gcaaatgtgg gaaatgttct ttcaccatct ccgtcagttg caggttggtt
    21241 tgcggatcgt cgatggcgtt aatcagcact tccgctttcg ccgctccggc agattccagt
    21301 aaatccatcc gcgtggcatc gccataaaac actttcatac cgaatttacg cagcgtttcg
    21361 atatggtccg ggtcgtgatc gagcaccacc attttcaccc cgctggagag cagtaaacgt
    21421 ccggttatct gcccaaaacg accgaatccg gcaatgatta cccgcggctg ttcttcgtcg
    21481 atctcatcgg cttcacgcgc ctcttcagta gaagattgct caaggcgatt gaggatcacc
    21541 agcagaatcg gcgttgccgc catcgacagt gccaccgcca gggtcagcga tttcgcccac
    21601 tctggctcca gcacattcgc catctgcgcc gcaccaaata ctacaaaggc aaactcactg
    21661 ccctgtccta ataacaccgc aaaccagcga cgctgtttat ttggcacctg caacggtcgg
    21721 gcaatcagcc acagcatggc gattttgatg atgaggaagc cgagcagcaa aataacaatg
    21781 cgcaacggat tttcaagcag cgtgccaaag tctatcgaca tgccaacgcc gatgaaaaac
    21841 agccccagca gcaaaccttt aaatggttcg atatcgcttt ccagcgcatg acggtattcc
    21901 gagcttgcca gcagtacgcc cgccagaaac gcgcccatcg ccatcgacaa gccgacctct
    21961 tccagcagca aaccaaagcc aaacacgagg aataacgcca cggcactaaa cacttcccgc
    22021 aagccagagc gggcaacaaa acgcagtgcc ggacgcgtga catagcgccc cagcaatacc
    22081 actagcacca gcgcgcccgc cacttttaac gccgagagaa caaatgcgcc catcgtcgtc
    22141 gaggcactgc tcgctgccag tagcgggatc atcgccacca gtgggatcgc cgcgatatcc
    22201 tggaacagca gcaccgcaaa ggcactgcgg cccatttgcg tcaccatcag attgcgttca
    22261 ttcatcgcct gcatggcaat cgccgttgat gagagcgcca gcgtcatgcc gatcaattct
    22321 gcgacctgcc agcgcagccc aagtaacata caaaacagcc ccagcaggcc gccgcaaatc
    22381 accatctgca atgcgccacc gccgaacact gccgcacgca gcttccacag cctttgtgga
    22441 tcgagttcga ggccgataat aaacagcatc agcaccacgc caatctctgc aaagtgcaga
    22501 atagattcgg catcggtcac cagtcgcagc ccccacgggc caataatgca gccggcgatc
    22561 aggtagccaa gaaccgatcc cagaccgaga cgtaccgcaa tgggaacaat cagcgccgcc
    22621 gaaccgagat aaatcagcgc ctgaataagc gtatggctat ccatgatgtg cctcctgcca
    22681 ttccagcaga cgttgcttat agtgccgcgc ctgcccttcg agggtttcgt cgtcacaaat
    22741 aaaggtgcag tgcatggcaa acggtggcag ccagttcagc ccgcagtaga ttgccgtcgc
    22801 ctgtagcggc tgcgacagca catcaaagcc cggatgcgca ccaatctcaa aatggctttc
    22861 cccaccgccg gtcgtcaccg cccacagcaa atgtttgccg tgcagcgccg tgccgccgtg
    22921 accgtaagcc cagccgtgcg agaaaacttt atcgatccaa agtttaagaa gcggtggaat
    22981 gctgtaccac tgcattgggt gctgccagac gatcagatcg gcacgagaca gcgcctcctg
    23041 ttcggcggca atgtcgatat tgaagtcagg atagagttga taaagagagc gaatttcgac
    23101 gccttccagc gtccttgcct gttcaagcat ccgtttattc gcatgggaat gatgcggata
    23161 tggatgcgca taaattataa gaatcatagg tagcccgcgt gattacgcct ttgttttccc
    23221 acaaagtgta gtcatatatt cacgcagctt atagtcaata atattgattc agaaaagaga
    23281 gaaatctgaa caaattaccc cattctctgc ccgcggatcg tcatattgct ggtctgcgcc
    23341 agtgacatcc ctcgagtctc cggggcaaac gctacggaaa tcaacaagcc aaacagcgag
    23401 atacccgccc ccattagcat cgtgttactg ataccgtaat tattgataaa gatcggcagt
    23461 gcccaggtcg aaacaatagt gccaatacgg cttaaggaca taatcacgcc cacggcagag
    23521 gcgcggatat ccgtcgggaa gagttcatta ggatagagcc actgcaaatt acccggcccg
    23581 ccagagaaaa aggcatacac cgcaaaggcc atcactacca gccagatccc catatccggg
    23641 atcagcccca aaactgccag cgccagcgtc atcatggcaa agctgccaat caacaatgga
    23701 cgccgtccgg cagtgtttag ccacagcatc ggcggaatac agccgagcat aaagaacagg
    23761 ctaatcacca cgttccccag tgccgcgttt ttgccaaccc ccaatcccaa caaaccaacg
    23821 atttgcgggc caaaggtgta aatggcgaac atagggatca cctggcaggt ccagatggcg
    23881 gcaacaaaca gaacaaaagg aaaatggcgg cgattaaaca gatcgcgaaa acgggtttgc
    23941 tgcggctgct cttcctcgaa agccaccggt tcgccaaaca gttttatcat catctcttcg
    24001 cactctttta ctcgcccttt gcgtaacaac cagcggggag attcaggcag ttcgaatcga
    24061 ccaatcaaaa tcaacaaaca gggaatcgct gcgctaccca gcatccagcg ccagccgcct
    24121 tccacatcat aaagccagta accgaccaga tcggcgcagg tcgcgccgac ataccacatc
    24181 gcggcgataa agctgatgga aaacgcccgc tgacgggtac tggagaactc ggtgatcatt
    24241 gaggtggcga taggataatc cgcaccgata acaatgccga taagtacccg catcaccaac
    24301 agttcgacgg gggatgagac aaacatcgtc gccaccgata tcacgccgat ggcgatgata
    24361 tcaatgagga acattttgcg ccgtccgact ttatcggaga tatagccaaa cagcgatgtg
    24421 ccaacgaaca gcccggcgag cgttcccgcg cccagcaagc caatccagtc agcgtccagt
    24481 ttcagcgccg gagtcagttg ctccagcgcc acgccaatca ttaccagtac ataaccatcc
    24541 agaaacggac caccgcttcc ccacagcaaa atgcggcggt gaatagagga gaatttaaga
    24601 tcatcaaagt ttctggacgg ttgcatgggt tattcctgtt tttttgcaat gagagtcagt
    24661 acggcattac gtatcaaacc aacatcagcc gtaacggaac tccacaccaa aggtgccgcg
    24721 cgggtattcc cactgttcca gcgccgaccc cagccccaga atgcgacagg tgccgcactc
    24781 cagacatccg gcgtaatcga agcgcacact gccgtcatcc tgcttcttgt acagacctgc
    24841 ggggcacgct tttaccagca gctccagggc ctgtttatcg gcatcggcct tcacaacgat
    24901 gtgcggatgc tcttcatcga cattgaattt attgacgccc agtttgacgt ccacattgac
    24961 gggagaagtc ataaaacggt cactcctttc atgccatcct tgatcagatt gatgaagccc
    25021 actttcttgc cgtggcggag gattttcttg cgcatcagtt ccggcgcact gccgtcaatg
    25081 gtgaacaggt cacgcgccac gcccaccgcc agttccgggt agccgctaaa catgcgtggg
    25141 ttatcaagga acgccggtag tttctggtac atgcgcatat cgcgcagcgg gccactctca
    25201 agatgctgac gatattccgc gagtttttgc ttactgaaat cgtcgctttt catcgctgaa
    25261 agcacagttt ttgctgctgc ttccccggcg gcaatcgcca gatccatacc gcgaatggta
    25321 aaaccgaggt tcatacacat tccggcggca tcaccggcaa tcagtacgcc gtcaccgacc
    25381 agctccggca gcatgttgat gcctgcttcc ggcactacgt gagcggaata ttccaccagc
    25441 ttgccgcccg ctatcagcgg tgcaacggcc ggatgttgtt tgaaatcttc cagcatttgc
    25501 ggcaccgatt tttttgcgtc atgcagatga tgcaaaccac aaaccagccc cagcgacagg
    25561 gtattttcat tggtataaag gaagccaccg cccatcaggc catcggtggg tgatcccgca
    25621 aacaggcaag ccgcgccctg attaccctgc aactgaaaac ggtcttcgat aaccgacttc
    25681 ggtaactcga tcagttcctt cacgccaacc gccacatccg tcggtttaac gcgttttgcc
    25741 atccccagct tttcggcaag gatggaattc accccatcag caaggatcac cgttttcgct
    25801 tcaatcacat cgccatcagc ttctacgccg acaactttgc catcgcgctg tacgaggtta
    25861 tcgacgcgga tcccagtaat taactgcgcg cccgcttctt cggcctgctc catcagccag
    25921 gcatcaaatt tactgcgcaa aacggagtaa gaacgctggg atggcgaggt ttcgtcgcca
    25981 ttgcagtagt ccatagtcat cgctgacttt tccgtcataa acgcaagttt ttcatgggtg
    26041 atcaggcgtt ctacgggggc ggagtcagcg aaaccaggaa taatgtgttc cagactgtgg
    26101 gcatagagac gcccgccggt gacgttcttg gcacctgcgg aattgccacg ctcgataact
    26161 aacacttgcg caccttcgcg ggcgagcacc agtgcggcaa ccgaaccggc aagccctgca
    26221 ccgacgatga tggcgtcaaa gatatcttcg gacataagag ctccctgtca gcggcagggc
    26281 caaaatgcag ccctgccaga gtggatcaac gcgctaaagc tgcggtcagt gccggaagga
    26341 ttttcacggc gtcgccaaca atgccgtaat ccgcgtactg gaagatcggc gcatttttat
    26401 ctttgttgat ggcgaaaatg gtttgcgacg cattagcgcc aaccatgtgc tggatctgcc
    26461 cggagatccc caccgccagg tacagttcag gtttcagcat caggttggag ataccgacat
    26521 agcgttcgtg ctccatccat ttttcgtttt ccgccaccgg acgagaacag gccagctccg
    26581 cacctatcgc cttacaaagc tgttctgcca gcgcaatgtt ctctttgctg ccaataccgc
    26641 gaccgacgct gaccaccagt cgggctttgt cgagatcgac gctgttgctc tgacgcgcct
    26701 gggttgccgt gcgggtaatc gccacagccg gagcctgcca ctccacggtg tgcgtttcgc
    26761 cagtgcgtga cgcgtctggc tgagccgcat caaacgtgcc gctgctgatg gtcagtaccg
    26821 catacggcgt ggcaatgcgt tcttcgccaa tcgccagacc gccgtaaacc atgtgtttca
    26881 ctgtcgcttt accgtcctgt acgctgacgg tgctggcatc gttagacacc gccgctttaa
    26941 ggcgataacc cagttttgcc gccagtaatt tgccgcgacg ggtgtttggc agcagcacca
    27001 gaccgtctgc gccgtgctgg cgaatagtgt cagccatgac accggcgtaa tcttcgatca
    27061 tccgatcgtc cggtttgccg tttaatttcc agacatgatt agcgccgagc tggattgcct
    27121 gtgcgccgtc ggcatcattg aggacaaagg cattgatttg attagctaaa gcctgcgcac
    27181 cgttcatcag ttccggcaga cgagaagggg tatcgctgaa tacccagact tgagaaaacg
    27241 tgttcatagc atcccctgta attaaatgac tttgcgaaga ttttcagcaa atgcggcgat
    27301 ctgttcttcg ccgtcgcctt caatcacgat gcgctgacgc tcgcgctgtt tcggcgctgc
    27361 aacctgttgt tctgaccagg ctgcctctgc gttaaaaccg atatccgccg ccgaccatac
    27421 ctggactggc tttttcgccg cgccgagaat ggctttcatc gaaggaattt gtggggagtt
    27481 gatatcagtg gaaacagcaa caaccgcagg cagcggaatg cttaaggttt cggtttcatc
    27541 ttccagttcg cgctcaacgg tgagggtatc tgccgtcagg gagataattt tgctgacgcc
    27601 gttaactgcc ggaatattga ggatttcgcc taccagcaaa ccaacctgct gggcataaag
    27661 gtcggaagaa ccatcgccac agaggatcaa atcaaagcct gctttctggg aggctgcagc
    27721 cagtgcgctc gccgtttgtt gcggcagtgc ctgctcgaac tggtcatcaa tcaccacaat
    27781 cagttcatcc ggaccgcgcg atagcacatc tttacgccct ttggcgttgg tcagggcttt
    27841 accgcccaca cttaaggccg tcacctgccc ctctgctgcc tgttgcttta gctggcaagc
    27901 cgcttcaata gcgttgagat cgtattggct tattttggca tcggctttac tgaagtctaa
    27961 cgaaccatca gcattattga ccgcaatatc ctgttcatca ggcacgcact tatagcaagt
    28021 aataatcttc attgcatctc cagaaatcat gaaggaaacg tcgttcatat cggtggtgtt
    28081 aattacagat atcacgccct ttaatttgga aataaaactt taatcaccaa tattgaaaat
    28141 gtcacacgca gataaatcca actttcaata ttgttaagtt cctcaccaat attgaaaaca
    28201 tggcgtagca aaaagaaatt ttcaatattg ctttatggat caccaatatt gaaagtcaga
    28261 tgctattttt ttactcacaa tcaaactcat cacattgata aacaatgaat atttacacaa
    28321 agacgccctg tttttacccg ataaaaaact gtgaccaaca tcacagaacg cagccaattg
    28381 aatacccatt atgagttagc cattaacgcg tccacgaggt taataataat tatattaaat
    28441 gttaacaaaa ataaaacaaa cgggaacgca aaataaatat tcgttttcac agtggaatta
    28501 actcatgaag aatgaaaaga gaaaaacggg aatagaaccg aaggttttct ttccgccgtt
    28561 aataatcgtc ggcatacttt gttggcttac agtcagagat ctggatgcag cgaatgtcgt
    28621 tattaatgct gtattcagtt acgtcaccaa tgtatgggga tgggcatttg aatggtatat
    28681 ggtagtgatg cttttcggtt ggttctggct ggtgtttggc ccgtatgcca aaaagcgttt
    28741 aggtaacgaa ccacctgaat ttagtaccgc cagttggatc tttatgatgt tcgcctcctg
    28801 tacgtctgct gccgtactgt tctggggatc gattgagatc tactactaca tctccacccc
    28861 gccgtttggc ttagaaccga actcgacagg ggcgaaagag ttggggctgg cttacagctt
    28921 gttccactgg ggacctctgc cgtgggccac ttatagcttc ctttctgtcg ccttcgctta
    28981 cttcttcttt gtccgcaaaa tggaagtgat tcgccccagc tccaccctgg tgccgctggt
    29041 aggtgaaaaa cacgccaaag ggttgttcgg cactatcgtc gacaacttct atctcgtcgc
    29101 cttgatcttc gcgatgggta ccagtctggg ccttgccacg ccgctggtga ccgagtgtat
    29161 gcaatggttg tttggcattc cgcataccct gcaactggac gctatcatca ttacctgctg
    29221 gattatcctc aacgccattt gcgtcgcttg cggtctgcaa aaaggggtac gtatcgccag
    29281 tgacgtgcgt agttacctga gcttcctgat gctgggttgg gtgttcatcg tcagtggtgc
    29341 cagcttcatc atgaactact tcaccgattc ggtggggatg ttgctgatgt atctgccgcg
    29401 catgttgttc tataccgatc ccatcgctaa aggcggcttc ccgcagggct ggaccgtgtt
    29461 ctactgggca tggtgggtga tttacgctat ccagatgagt atcttcctcg cccgcatctc
    29521 ccgtggtcgt accgtgcgtg aactgtgctt cggcatggtg ctggggctga cagcgtcaac
    29581 ctggatcctg tggactgtac tcggtagtaa cactctgctg ttgatagata aaaacatcat
    29641 caacattcca aatctgatcg aacagtacgg tgtggcgcgc gccatcatcg aaacctgggc
    29701 cgctctgcca ctcagcaccg ccaccatgtg gggcttcttc atcctctgct ttattgccac
    29761 cgttacgctg gttaacgcct gctcttatac cctggcgatg tccacttgcc gcgaagtgcg
    29821 tgacggcgaa gaacccccgc tgctggtgcg cattggttgg tcagttctgg ttggcattat
    29881 cggcattgtt ctgctggcgc tcggcggcct gaaaccgatt caaaccgcca ttatcgccgg
    29941 aggatgcccg ctgttcttcg tcaacatcat ggtgacgctc tcctttatta aagacgcgaa
    30001 acagaactgg aaagattaat taacccccaa aatatcaaga ggttgaaaga tggattttaa
    30061 tttaaatgat gagcaggaac tgtttgtcgc cggtatccgc gaactgatgg ccagcgaaaa
    30121 ctgggaggcc tattttgccg agtgcgaccg tgacagcgtc tacccggaac gttttgtcaa
    30181 agccctggcg gatatgggta tcgacagtct gctgatcccg gaagaacacg gtggtctgga
    30241 cgcggggttt gttactctcg ccgccgtgtg gatggaactg ggtcgtctgg gagcgccaac
    30301 ttacgtgctg tatcagttac cgggcgggtt taacaccttc ctgcgtgaag gtacgcaaga
    30361 gcagatcgac aagatcatgg ctttccgcgg caccggtaag cagatgtgga actcagcaat
    30421 taccgaaccg ggcgcaggct ccgacgtggg tagcctgaaa acgacttata cccgtagaaa
    30481 tggtaagatt tatcttaatg gtagtaagtg ttttattacc agtagcgcct acaccccgta
    30541 catcgtggtg atggcgcgcg acggggcttc tccggacaaa cctgtctaca ccgaatggtt
    30601 tgttgatatg agcaaaccgg gcatcaaagt gaccaaactt gaaaagctcg gtctgcgtat
    30661 ggatagctgc tgtgaaatca cttttgacga cgtggaactg gacgagaaag acatgttcgg
    30721 tcgggaaggt aacggcttta accgcgtcaa agaagagttc gaccatgaac gtttcctggt
    30781 agccctgacc aactacggta cggcgatgtg cgcctttgaa gatgcggcgc gctacgccaa
    30841 ccagcgtgtg cagtttggcg aggctattgg tcgtttccag ttgattcagg aaaaattcgc
    30901 ccacatggcg atcaaattaa actccatgaa aaacatgctg tatgaagcag cgtggaaagc
    30961 agacaacggc accatcacct ctggcgatgc agcgatgtgc aaatacttct gcgccaatgc
    31021 ggcatttgaa gtggtggata gcgcaatgca ggtgctgggc ggtgtcggga ttgcgggcaa
    31081 ccaccgcatc agccgcttct ggcgtgacct gcgtgtagac cgcgtttccg gaggatctga
    31141 cgaaatgcag atcctgacgc tgggtcgcgc ggtgctgaag caataccgct aagtccgtgc
    31201 gctgcctgat gcgacgctga cgcgtcttat cagacctacg ggagcacgaa tgtaggccgg
    31261 ataaagcgtt tacgccgcat ccggcagtca tgcgctacat aaatttttca ggagctaata
    31321 atggatcatc tacccatgcc gaaattcggg ccgctggccg gattacgcgt tgtcttctcc
    31381 ggtatcgaaa tcgccgggcc gttcgccgga caaatgttcg cagaatgggg ggcggaagtt
    31441 atctggatcg agaacgtcgc ctgggccgac accattcgcg ttcaaccgaa ctacccgcag
    31501 ctctcccgcc gcaatttgca tgcgctgtcg ttaaatattt tcaaagatga agggcgcgaa
    31561 gcgtttctga aattaatgga aaccaccgat atcttcatcg aagccagtaa aggtccggcc
    31621 tttgcccgtc gtggcattac cgatgaagtc ctgtggcagc acaacccaaa actggttatc
    31681 gctcaccttt ccggttttgg tcagtatggc accgaggagt acaccaatct tccggcctat
    31741 aacactatcg cccaggcctt cagtggttac ctgattcaga acggtgatgt tgaccagcca
    31801 atgcctgcct tcccgtatac cgccgattac ttttctggcc tgaccgccac cacggcggcg
    31861 ctggcagcac tgcataaagt gcgtgaaacc ggtaaaggcg aaagcatcga catcgccatg
    31921 tatgaagtga tgctgcgtat gggccagtac ttcatgatgg attacttcaa cggcggcgaa
    31981 atgtgcccgc gcatgaccaa aggtaaagat ccctactacg ccggttgcgg tctgtataaa
    32041 tgcgccgacg gctacatcgt gatggaactg gtgggcatta ctcaaattgc agagtgcttt
    32101 aaagatattg gcctcgcaca tctgctcggt acgccggaaa tcccggaagg cactcagctt
    32161 atccaccgta tcgaatgccc ttacggccca ctggtagaag agaaactcga tgcctggctg
    32221 gcggcacata ccatcgcaga agttaaagaa cgctttgccg aactgaatat cgcctgtgcc
    32281 aaagtactga ccgtgccgga actggaaagc aatccacagt atgtggctcg cgaatcaatc
    32341 actcagtggc aaacgatgga tggtcgcacc tgcaaagggc cgaatatcat gccgaaattc
    32401 aaaaataacc ccggacaaat ctggcgcgga atgccctcac atggcatgga cacggctgcc
    32461 attttgaaaa atatcggcta cagcgaaaac gacattcagg agttggtcag caaaggtctg
    32521 gccaaagttg aggactaaac gcgtggcgtc agtcaacttg cctgactagc gcccgcccaa
    32581 taacaaacga acaaaatgga tagaggtgca atggatatca ttggcggaca acatctacgt
    32641 caaatgtggg acgatctggc ggacgtttac ggtcataaaa cggcgctgat ttgtgaatcc
    32701 agcggcggag tcgttaaccg gtatagttat cttgagttaa atcaggagat taaccgcacg
    32761 gcaaacctgt tttatacgct ggggattcgc aaaggcgaca aggttgctct acatctcgac
    32821 aactgcccgg aatttatctt ttgctggttc gggttggcaa aaattggcgc gattatggtg
    32881 ccgattaacg cccgcctgtt gcgcgaagaa agcacgtgga tcctgcaaaa tagccaggcg
    32941 tgcctgctgg tgaccagtgc gcaattttat cctatgtatc aacagattca gcagaaagat
    33001 gccacgcagt tgcggcacat ttgcctgaca gatatggcac ttcccgctga tgatggcgtg
    33061 agttcgttta ctcaactgaa aaatcaacaa cctgccacct tgtgctatgc accgccgcta
    33121 tcgactgacg atacggcgga aattctcttc acctccggca ccacctcccg accgaaaggg
    33181 gtggtgataa ctcattacaa cctgcgcttc gctggatatt actccgcctg gcagtgtgca
    33241 ctgcgtgacg atgacgtcta cctgacggta atgcctgcgt ttcatatcga ttgccagtgt
    33301 actgcggcga tggcggcgtt ttctaccggg gccacctttg tgctggtcga gaaatacagc
    33361 gcccgtgcct tctggggaca ggtgcagaag taccgcgcca ccattaccga atgtattccg
    33421 atgatgatcc gtacgttgat ggtgcagccg ccttcagcga acgatcagca acaccgcctg
    33481 cgggaagtga tgttttatct caacttgtcg gagcaggaaa aagacgcatt ttgtgaacgc
    33541 ttcggcgttc gcttgctgac gtcttatggg atgacggaaa ccattgtggg cattatcggc
    33601 gatcgccctg gcgataaacg acgctggccg tcgattggtc gggcggggtt ttgctacgaa
    33661 gcggagatcc gcgacgatca caatcgcccg ctcccggcag gtgagatcgg tgaaatctgt
    33721 attaaaggcg taccagggaa aaccatcttc aaagagtatt ttctcaaccc gaaagccact
    33781 gcgaaagtac tggaagccga tggctggcta cataccggcg ataccggata ccgcgacgaa
    33841 gagggctttt tttatttcgt cgatcgccgc tgcaatatga tcaaacgcgg tggcgagaat
    33901 gtctcctgcg tggagctcga aaatatcatc gccacccatc ccaaaattca ggatatcgtg
    33961 gttgtgggta ttaaagattc gattcgcgat gaagccatca aagcatttgt ggtgctgaat
    34021 gaaggtgaaa cattgagcga agaggaattc ttctgcttct gtgaacagaa tatggcgaaa
    34081 tttaaagtgc cctcttatct ggagatcaga aaagatctgc cacgtaattg ctcggggaaa
    34141 ataattagaa agaatctgaa ataaatgaaa cggcagggaa ctaccctgcc agctaataat
    34201 cacgctctaa aacaatatgc attttttgca gggatgttgt cgtccctgaa aaagcaaaaa
    34261 tggagaaaag gaatgagtga atcattacat ctgacccgca atggatcaat tctggaaatt
    34321 acccttgatc gtccaaaagc gaatgctatt gatgcaaaaa ccagctttga aatgggcgaa
    34381 gtatttctaa atttccgtga cgatccgcaa ttacgtgtcg ccattattac cggtgccgga
    34441 gagaaattct tttccgcggg ctgggattta aaagcggcag cagaaggcga agcaccggat
    34501 gctgactttg gcccgggagg ttttgcggga ttaaccgaaa ttttcaatct cgacaaaccg
    34561 gtcatcgccg ctgtgaacgg ctatgccttt ggcggcggct ttgaactggc gctggcggca
    34621 gattttattg tttgtgccga taacgccagc ttcgccctac cggaagccaa actgggcatc
    34681 gttcctgaca gcggcggcgt gctgcgtctg ccgaagatcc tgccgcctgc catcgtcaat
    34741 gaaatggtga tgaccggcag acgaatgggc gcagaagagg cgctgcgttg gggggtagtc
    34801 aaccgcgtgg ttagccaggc ggaactgatg gataacgccc gcgaactggc gcagcagctg
    34861 gttaacagcg ctccgctggc gattgcggcg ctgaaagaga tctaccgcac caccagtgaa
    34921 atgccggtag aagaagcgta tcgctatatt cgcagcggcg tgttgaaaca ctatccatcg
    34981 gttctgcatt cggaggatgc cattgaaggg ccgctggcgt ttgccgagaa gcgcgatcca
    35041 gtgtggaaag gacgttaaca accgtgagct attacgcctt tgaggggtta attccggttg
    35101 ttcacccgac ggcgtttgtc catcccagcg cagtcttgat tggcgatgtg attgtgggag
    35161 ccggtgtcta catcggccca ctcgcctcac tgcgtggtga ctacgggcgg ttgatcgtgc
    35221 aagcgggagc caatattcag gatggctgca ttatgcatgg ctactgcgac actgacacca
    35281 tcgttgggga aaacggtcat atcgggcacg gagcgatcct gcatggttgt gtgattggtc
    35341 gcgatgcact ggtcggtatg aacagcgtga ttatggatgg cgcggtcatt ggcgaagaga
    35401 gcattgttgc cgctatgagc tttgtcaaag cgggctttcg cggcgagaaa cgccagttgt
    35461 tgatgggtac gccagcccgc gctgtacgca gtgtgagtga cgacgagtta cactggaaac
    35521 ggttgaatac caaagagtat caggatcttg tcgggcgctg tcatgcatcg ttacatgaaa
    35581 cgcagccact gaggcaaatg gaggaaaatc gcccccgttt gcaggggacg acggatgtga
    35641 cgccgaaacg gtgatctttc cggatgcggc gtaaacgcct tatccgacct ggctgtaaag
    35701 caaccgtagg ccggataaga tgcgccagca tcgcatccgg cgaccaggtc gcacggtatc
    35761 aacgaaatga cattaacgac gcatactctt tgacaacatc atctgccact tctgctcgcg
    35821 gttaagttcc gtgagcggaa cggcaggaat acgcggcgtc tttcttacgc cgactaattt
    35881 atcccggctg ttattgcgca ggcgcgcgta aatttgctca tcgatatcaa ccacttttac
    35941 cagtcgctgg cactggcatc cccgcccttc cagcttatta gggatcatct taacttcgca
    36001 gcttatttct gtgacttccg acagaatata agtcagggta ttaaccgcct tgctgtgttc
    36061 aatatcgaaa tgaatagaga tctctcttgc tatcacccac cggttttcag ccatcatcca
    36121 ttcggcgatt aacaaataga gtggtttttc aacacatcct tcacacatac gaactccagt
    36181 ttcacaaatt aaaagactgg ctatgactcg ttttgcagca attaaacaag gccatattaa
    36241 aattgtggat atatcgatag tatgcaactt caccgtcgat aagtgtgagc taaatcctgc
    36301 ttatgtcatc aaaaatatcg attccccaaa agatttactt aatgggcaaa aaaaacggtc
    36361 ctcatcagag aaccgtatta gttattcgat caaattagcg gatgaaaaat atccgccatg
    36421 acacgctatt atttgatctg cgcgtgcatt tcctgcaccg aaattacttt ttcggtcgca
    36481 tcggcattca gcgccatcgc ggtggcaaag ccgccgttca gggtggtgtc gtaatgcact
    36541 ttatattgca gcgcactgcg acggatcacg cgggagtctt caatcgcacg acgacctgag
    36601 gtggtattga tgatgtaggt atattcgcca ttcttaatac ggtcctgaat gtgcggacgg
    36661 ccttcatgca ccttgttcac cagacgcgga ttgatacccg cttcgcccag cacaatcgcc
    36721 gtgccgtgag tcgcatccag ctcgaagccc tgtttcagca gttttgccgc caggtccacc
    36781 acgcgttctt tatcgccttc gcgcacggaa agcagcgcac gaccgtgttt cttcatggtg
    36841 gagttgctgc ccagctgcgc tttggcaaac gcttcagcga aggtgcggcc cacgcccatt
    36901 acttccccgg tagagcgcat ttctggccct aacagcgggt caacgcccgg gaatttattg
    36961 aacggcagca ccacttcttt caccgagtag tacggcggga taacttcttt ggttacgccc
    37021 tgctcagcca gcgatttgcc agccatcaca cgcgccgcca cttttgccag cggtacgccg
    37081 gtggctttgg agacgaacgg aacggtacgc gccgcacgcg ggttaacttc aatcaggtag
    37141 acttcgttgt ttttcaccgc aaactgcacg ttcatcaggc cgcgcacctg cagttcgaag
    37201 gccagtttct gcacctgctg gcgcatcaca tcctgaattt cctgacttaa ggtgtaagct
    37261 ggcagtgaac atgcggagtc accggagtgc acacccgcct gctcaatatg ctccatgatg
    37321 ccgccaatca gcaccatttc gccgtcgcag atggcatcca cgtcaacttc tactgcgtca
    37381 tcaaggaagt ggtccagcaa cactggcgca tcgttagata cgctgaccgc tgtctggaag
    37441 tagcgacgca ggtcagcttc gtcatagacg atttccatcg cccgaccgcc gagaacgtaa
    37501 gacggacgca ccaccagcgg gtagccaatc tctttcgctt tctcaaccgc catttcgatg
    37561 gtggtaacgg tggcgttcgc cggttgtttc agtttcagac gctcaaccgc atgctggaag
    37621 cgttcacggt cttccgcacg gtcgatggca tccgggctgg taccgataac cggtacgcca
    37681 gcagcttcca gcgcgcgcgc cagtttcagc ggggtctgac cgccgtactg aacgataacg
    37741 cctttcggct tctcaatacg cacaatttcc agcacgtctt ccagcgttac cggttcgaag
    37801 tagaggcggt cggaggtgtc gtagtcggtg gagacggttt ccgggttaca gttaaccata
    37861 atggtttcgt aaccgtcttc acgcagcgcc agcgaggcgt gtacacagca gtagtcgaat
    37921 tcgataccct gaccgatacg gttcgggccg ccgccgagca ccatgatttt ttcacggtcg
    37981 gtagacggat tcgcttcgca ctcttcttca taagtggagt acatgtaagc ggtgtcggtg
    38041 gcgaactctg ccgcacaggt atccacgcgc ttataaaccg ggtgcaggtc atactggtcg
    38101 cgcagcttgc ggatttctgc ttcacgcacg cccgcgagtt ttgccagacg cgcatcggca
    38161 aagcctttgc gtttcagctg gcgcaggaag tcagcgttga ggccagtgat acccacttct
    38221 gcgactttct cttccagacg caccagttct tcaatttgta ccaggaacca gcggtcaata
    38281 ttggtcaggt taaagacgcc gtctacagac aagcccgcac ggaaggcatc ggcgatgtac
    38341 cagatacgct cagcgcctgc atctttcagt tcgcggcgga ttttggtcag ggcttccggg
    38401 tcgtcgaggc tcactttcgg gtcgaagcca gtcgcgccca cttcgaggcc gcgcagggct
    38461 ttttgcagag attcctgctg cgtgcgtcca atcgccatca cttcgccaac cgatttcatc
    38521 tgggtggtca gacggtcgtt cgcaccggcg aatttctcga agttaaagcg aggaattttg
    38581 gtgaccacat agtcgatgga cggttcgaag gaggccggag tacgcccgcc ggtgatgtcg
    38641 ttcatcagct cgtcgagggt gtaacccacc gccagtttcg ccgccacttt ggcaatcggg
    38701 aagccggtgg ctttcgacgc cagcgccgaa gaacgcgaca cacgcgggtt catttcgata
    38761 acaatcaggc gaccgttctt cgggttcacc gcaaactgga cgttagaacc accggtttca
    38821 acgccgattt cacgcagtac cgccatcgag gcgttacgca tgatttggta ttctttgtcg
    38881 gtcagtgttt gcgctggcgc tacagtaata gagtcaccgg tgtggatacc catcgcgtcg
    38941 aagttttcga tagagcaaac gatgatgcag ttgtcgtttt tatcacgcac cacttccatc
    39001 tcgtactctt tccagccgat cagcgactca tcaatcagca gctctttggt cggggagaga
    39061 tccaaaccgc gggcgcaaat ttcttcaaat tcttcacggt tataagcgat accgccaccg
    39121 ctaccgccca tggtaaagga tgggcgaata atgcacggga agccaacttc agcggcaacc
    39181 gccagcgctt cttccattga atgagcgata ccggaacgcg cagtttccag accgattttc
    39241 ttcatcgcta cgtcgaaacg gcggcggtct tcggctttat caatcgcatc ggcagtggca
    39301 ccaatcatgg tgacgccgaa ctcttccagc acgccctgac gctcaagctc cagcgcacag
    39361 ttcagcgccg tctgcccccc catggttggc agcaccgcgt ccgggcgctc tttttcaata
    39421 atcttgcgta ccacttccca gtgaatcggc tcgatgtagg ttgcatcagc catttccggg
    39481 tcggtcatga tagtcgccgg gttggagttc acgagaatga cgcggtaacc ctcttcacgc
    39541 agggctttac acgcttgcgc gccagagtag tcaaactcac acgcctgacc gataacaatc
    39601 gggcccgcac ccagaatcag gatacttttt atatctgtac gttttggcat ggctctttta
    39661 ctcctgatta cttagcggtt ttacggtact gctcaattaa cgcgataaag tggtcaaaca
    39721 acggcgcggc gtcgtgtgga ccagggctgg cttccgggtg tccctggaag ctgaacgccg
    39781 gtttatcggt acgatgaatg ccctgtaacg taccgtcgaa cagggattta tgtgtgacac
    39841 gcaggtttgc aggtaatgtt gcttcatcca ccgcaaaacc gtggttctgg gcggtgatca
    39901 tcaccacgtt tttctcaaca tctttaaccg gatggttgcc gccgtggtga ccaaatttca
    39961 ttttgatggt cttcgcaccg ctcgccagcg ccagcagctg atgaccgaga cagatgccga
    40021 ataccggaat atcggtttcg aggaatttct ggatggcggt aatggcgtaa tcgcacgggg
    40081 ccgggtcgcc aggaccgttg gagagaaaga tgccgtctgg attcattttc agcacatctt
    40141 ccgcagaagt ttgcgccgga acgatggtca ggcgacagcc tctatccacc agcatacgca
    40201 ggatgttgcg cttggcaccg aaatcgtagg ccacgacgtg gtacggcagc tcgtcttctt
    40261 ttttcgcttc tggcaggccg ccggtcagtg tccagctccc ttgtgtccag ctatagggtt
    40321 ctgcggtggt tacttctttt gccagatcca tgccgttcag acctgggaac gcgcgggctt
    40381 tttctaacgc cagcgccgca tccgggttat cgcctgcgat aatgcagcca ttctgagcac
    40441 ctttctcacg cagtaaacgc gtcagcttac gggtatcgat atcggcaatc gccacgatgt
    40501 tatggcgctt caggtaagaa gagaggtctt cggtattacg gaagttgctg gcaatcagcg
    40561 gcaggtcgcg aatcaccaga ccttgggcat gaacctgaga agattcttca tcggcgtcat
    40621 tagtgccgac attgccaata tggggataag taagagtaac gatttgacga gaataggaag
    40681 gatcagtgag gatttcttga taaccggtca ttgaagtatt gaaaacgact tccccaaccg
    40741 ccgaacctgt tgcccctatg gcccgaccgt gaaactgggt tccgtcttcc agaaccaata
    40801 gcgctgactt aatcaaaaca ccctccagag aatattcact cactttattt gcatattaat
    40861 tcacaatgat gacgtaaatc aatgcaaatc tgcttaccga cgaatttctg gcaaacggcg
    40921 gcattctgga gatatgggag ctaaaagtca acttaatggc gggatttttt atattatttt
    40981 gtggctcatg catgttttac aggcattaag cgacaaaaag atcatataac atgttctgga
    41041 aaacgcttgc gcagcaagaa gattgtatga aatcaaaatg agcagaaaaa agtggaccaa
    41101 atggtcaaaa tttacattaa aacaacaaaa acagaataca aaacatgtaa ttttcagcat
    41161 tttcattaaa tttaaaagca taacactaaa aa
//
LOCUS       DAZFJL010000022        40653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFJL010000022 DAZFJL010000000
VERSION     DAZFJL010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.022"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..424
                     /gene="traP"
                     /locus_tag="ACHJ8C_001203"
     CDS             <1..424
                     /gene="traP"
                     /locus_tag="ACHJ8C_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFO5551096.1"
                     /translation="KLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYR
                     AADAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVLPGKMKCIRVVTADEY
                     LKDVYTEDASLPGEIRRCHVYFPDDRTNRSNGDKNEHA"
     gene            411..731
                     /locus_tag="ACHJ8C_001204"
     CDS             411..731
                     /locus_tag="ACHJ8C_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFO5551097.1"
                     /translation="MNMRNINVITARVVPDKNVSDDFMHAVLSNCTTKIVLPAPKEFS
                     SESLPHNFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGETNLTAELISAVVGSNKK
                     GEGQ"
     gene            728..1243
                     /gene="traV"
                     /locus_tag="ACHJ8C_001205"
     CDS             728..1243
                     /gene="traV"
                     /locus_tag="ACHJ8C_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFO5551098.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTITATTPSGSRPAVTTLPEQKLLTPR
                     PLFTAAREVKTVVPVSSVAPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            1378..1599
                     /gene="traR"
                     /locus_tag="ACHJ8C_001206"
     CDS             1378..1599
                     /gene="traR"
                     /locus_tag="ACHJ8C_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFO5551099.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            1592..2068
                     /locus_tag="ACHJ8C_001207"
     CDS             1592..2068
                     /locus_tag="ACHJ8C_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5551100.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQSLLQLMRLTEDVPDA
                     RQELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFTEENKSE"
     gene            2148..2366
                     /locus_tag="ACHJ8C_001208"
     CDS             2148..2366
                     /locus_tag="ACHJ8C_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5551101.1"
                     /translation="MHTFIFLVLIKNINGGGSWNVTPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPLNNVMETLNGGGQ"
     gene            2394..2756
                     /locus_tag="ACHJ8C_001209"
     CDS             2394..2756
                     /locus_tag="ACHJ8C_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5551102.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMLICGLFYNDYLEQQPATPEQLTEI
                     TQDIPCAAEAFKEAIKSDTSDYQPEPLSLGKAKELASACRERNEMAEVKRVRENERNK
                     IREKQIQALNDAHSVKER"
     gene            2882..5512
                     /gene="traC"
                     /locus_tag="ACHJ8C_001210"
     CDS             2882..5512
                     /gene="traC"
                     /locus_tag="ACHJ8C_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFO5551103.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASLQGASITTQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNVADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            5509..5895
                     /gene="trbI"
                     /locus_tag="ACHJ8C_001211"
     CDS             5509..5895
                     /gene="trbI"
                     /locus_tag="ACHJ8C_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFO5551104.1"
                     /translation="MTTTQKTTDVTARRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTVAFNMKQTVDAFFDSANQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            5892..6524
                     /gene="traW"
                     /locus_tag="ACHJ8C_001212"
     CDS             5892..6524
                     /gene="traW"
                     /locus_tag="ACHJ8C_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFO5551105.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKETDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            6521..7513
                     /gene="traU"
                     /locus_tag="ACHJ8C_001213"
     CDS             6521..7513
                     /gene="traU"
                     /locus_tag="ACHJ8C_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFO5551106.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGTCAADAIASAFNMPLDALFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            7522..8160
                     /gene="trbC"
                     /locus_tag="ACHJ8C_001214"
     CDS             7522..8160
                     /gene="trbC"
                     /locus_tag="ACHJ8C_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFO5551107.1"
                     /translation="MKLSMKSLAALLMMLNGAVMASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWTEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRYYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVARALLNNPGDKQK"
     gene            8157..8537
                     /locus_tag="ACHJ8C_001215"
     CDS             8157..8537
                     /locus_tag="ACHJ8C_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5551108.1"
                     /translation="MTLPAGYYRIDPEIRALVAAMNIHGFRTYASCQGHGFPVTKLLP
                     YIAFVCPVKMAALLEQRLRQDAESAIPRLTWGWSVKGAFNSEFQLCFRLQPDAPHYWY
                     NRYCRHSLCADFRTLISLLKSLSE"
     gene            8773..10635
                     /gene="traN"
                     /locus_tag="ACHJ8C_001216"
     CDS             8773..10635
                     /gene="traN"
                     /locus_tag="ACHJ8C_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFO5551109.1"
                     /translation="MKEKINTFFRSPNFNYSVKPCNLCAIVFFLSFSVFADSNSDYRA
                     GSDFAHQIKGQGSSSIQGFKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWA
                     TGETGKTITESFMNKPKDILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEY
                     TNYTCERDLQVEQYCTRTARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPV
                     TGEIVDAHYSWSRTYLQKSVPMTITVLGTPLSWNAKYSADASFTPVQKTLTAGVTFTS
                     SHPVRVGNTKFRRHTAMKLRLVVRVKKASYTPYVVWSESCPFSKELGKLTKTECTEAG
                     GNRTLVKDGQSYSMYQSCWAYRDTYVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEG
                     ACLHEYATYSCESKTSGKVMVCGGDVFCLDGECDKAQSGKSNDFAEAVSQLAALAAAG
                     KDVAALNGVDVRAFTGQAKFCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKS
                     NKLTVSVGEFCSKKVLGVCLEKKRSYCQFDSKLAQIVQQQGRNGQLRISFGSAKHPDC
                     RGITVDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            10662..10919
                     /locus_tag="ACHJ8C_001217"
     CDS             10662..10919
                     /locus_tag="ACHJ8C_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFO5551110.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTVGGML
                     AAAFVIFYSGVLLFILYFCFSVLTDLQKPDERKSDERNEDE"
     gene            10912..11655
                     /gene="traF"
                     /locus_tag="ACHJ8C_001218"
     CDS             10912..11655
                     /gene="traF"
                     /locus_tag="ACHJ8C_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFO5551111.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLEKQFLNVSEDFKPNF"
     gene            11671..12018
                     /gene="trbA"
                     /locus_tag="ACHJ8C_001219"
     CDS             11671..12018
                     /gene="trbA"
                     /locus_tag="ACHJ8C_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFO5551112.1"
                     /translation="MSEDYLKMFTDVVLLIFVIIAGYFFSERNVRKMFLLSSLVFLVI
                     NIACLYVLTASLWFLCGAIMSQSAALVVTMVIGSLPNVTSFDRFRRIFICIMLSSVWS
                     GMMWFFIRGLMTG"
     gene            complement(12020..12334)
                     /locus_tag="ACHJ8C_001220"
     CDS             complement(12020..12334)
                     /locus_tag="ACHJ8C_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFO5551113.1"
                     /translation="MEKRSFKEKLEIIRNIIRESLLGNAAIIALIYAASHSLPVNAFP
                     DHLVISLLSIAAGIVVLWLFSIIYIYFCKLFRSHWIAVWFIIWSSVINLIILHGFYDR
                     FI"
     gene            12415..12699
                     /gene="traQ"
                     /locus_tag="ACHJ8C_001221"
     CDS             12415..12699
                     /gene="traQ"
                     /locus_tag="ACHJ8C_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFO5551114.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMAEQQEGGHVSH"
     gene            12686..13231
                     /gene="trbB"
                     /locus_tag="ACHJ8C_001222"
     CDS             12686..13231
                     /gene="trbB"
                     /locus_tag="ACHJ8C_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFO5551115.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAAQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARMDTVLQMYGGKKGAK"
     gene            13161..13478
                     /gene="trbJ"
                     /locus_tag="ACHJ8C_001223"
     CDS             13161..13478
                     /gene="trbJ"
                     /locus_tag="ACHJ8C_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFO5551116.1"
                     /translation="MPPVLWRGWIPFCRCTEEKKVRNKQMVLFIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMTGLNESS
                     EIS"
     gene            13489..13881
                     /gene="trbF"
                     /locus_tag="ACHJ8C_001224"
     CDS             13489..13881
                     /gene="trbF"
                     /locus_tag="ACHJ8C_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFO5551117.1"
                     /translation="MRENKNNPELKIRSTERDYKYISGITDRYAGLSLVFLTAGIVLW
                     TVMNIIFDACIDSWKADPELNNSIYMWNILMYAIPCTLWALASGFFVAGYLLPLCALA
                     VKNIMIFRLKRRMRRENTLREGSNNASH"
     gene            13868..15241
                     /gene="traH"
                     /locus_tag="ACHJ8C_001225"
     CDS             13868..15241
                     /gene="traH"
                     /locus_tag="ACHJ8C_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFO5551118.1"
                     /translation="MLRIKPLFILCAALLTLTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIGG
                     LFPRTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKAGDKDKERVTKNI
                     NIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRAMMEGG
                     TAKIYHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKGF
                     ISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNYD
                     EAVIEHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            15238..18057
                     /gene="traG"
                     /locus_tag="ACHJ8C_001226"
     CDS             15238..18057
                     /gene="traG"
                     /locus_tag="ACHJ8C_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFO5551119.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSVTYSKTGMLFGAELVSKSTDFLSRNPEIANLFQDY
                     VQNCVMGDIYLNHKYTLEELMASADPYTLIFSRPSPLRGVYDSNNNFVTCKDASVSLK
                     DKLNLDTQSGGKTWHYYAQQLFGGRPDPNLLFSTLIGDSYSYFYGSSKSASQIIRQNV
                     TINALKEGITSYAARNGDSASLVNLATTSSMEKQRLAHVSIGHVAMRTLPMTQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQSWPMLYAILNSAMTFYAKQNGAPV
                     VLSEISQIQLKYSDLASTAGYLSMMIPPLSWMMVRGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNMLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATRELASRSTSVSAGLY
                     GDVHLEKNFKPSFMGVGGGIGVKAGVKGSIDGSDLDSHEASSGSRASHDARHDIDAKA
                     SKDFKEASDYFMNRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKYAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGGIGKGMDSVPLGGGRQDIIADYQGHQAIIEQRTQDSNIRNN
                     VKQQVDNMVRDYRSEIDTVNKSIRNDEGSIRQQYNALQTHHKSEELSQDRKYNEEKAA
                     QERMPGADTPDELQKKAREYLGKNS"
     gene            complement(18060..18368)
                     /locus_tag="ACHJ8C_001227"
     CDS             complement(18060..18368)
                     /locus_tag="ACHJ8C_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO5551120.1"
                     /translation="MGLFDHKTNITISAHSNVFTSTPSQMKIVKNFGLIKATTAEISG
                     KYYNEDDELLSALLSEASKMGANAIVNFQYISGSYNTGNGYFATYIIATGDAVVLEDI
                     "
     gene            18583..19179
                     /locus_tag="ACHJ8C_001228"
     CDS             18583..19179
                     /locus_tag="ACHJ8C_001228"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5551121.1"
                     /translation="MDIKKNIIIAFATTLLGGCGSIGNIKGVEPSKYAHVTDYLNDIF
                     EKSNCIGTVFSPQGRISTDADGLKYQLNGENIRCYSDSIENAVEKYCVYKGGEIVQGG
                     RWCKVNGEPLFYINYGYDGKHYGAPKENFMVVEQGSFQGRQQWLIADERLGFLSERVL
                     LQQKLENEINAKKRSRRVTTKNKRKKYTCECGCWGFDL"
     gene            19597..21750
                     /gene="traD"
                     /locus_tag="ACHJ8C_001229"
     CDS             19597..21750
                     /gene="traD"
                     /locus_tag="ACHJ8C_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO5551122.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYARKRGDMVVI
                     YDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTYLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHSGESFTIRDWMRGVREDKQNGWLFISSNADTHA
                     SLKPVVSMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMESRLSAVLAAREAEGRQMASLFEPDVTEVVSGEDVTQAEQPQQ
                     PVSPAINDKKSDSGVNVPAGGIEQALKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQ
                     QENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(21759..22157)
                     /gene="vapC"
                     /locus_tag="ACHJ8C_001230"
     CDS             complement(21759..22157)
                     /gene="vapC"
                     /locus_tag="ACHJ8C_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HFO5551123.1"
                     /translation="MLKFMLDTNICIFTIKNKPTSVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELSRQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVDGLRIEDWS"
     gene            complement(22157..22384)
                     /gene="vapB"
                     /locus_tag="ACHJ8C_001231"
     CDS             complement(22157..22384)
                     /gene="vapB"
                     /locus_tag="ACHJ8C_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFO5551124.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ESWDEWFDGNSVSADFMDNREQPGMQERESF"
     gene            22466..27736
                     /gene="traI"
                     /locus_tag="ACHJ8C_001232"
     CDS             22466..27736
                     /gene="traI"
                     /locus_tag="ACHJ8C_001232"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFO5551125.1"
                     /translation="MMSIAQVRSAGNAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHSGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEIKMAE
                     WMQTLRETGFDIRAYRDAADQRAETRTLTPGPASPDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMRDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRRHDRYVIDRVTAQSHSLT
                     LRNAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPASLPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMVMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KARLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHNQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWETHQDALALVDNVYHWIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRELGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRMIRPARERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRIKGSEDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPLTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTELAVREIAGQERDRAAITEREAALPESVLREPQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            27756..28502
                     /gene="traX"
                     /locus_tag="ACHJ8C_001233"
     CDS             27756..28502
                     /gene="traX"
                     /locus_tag="ACHJ8C_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFO5551126.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGMNLSCHAHIRQPAINRLWEWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRSAAAILLMALWGPLSGTSYSIAG
                     LLMLAVSRRLYRAEDRTERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     TGKSLPRFWYGDFFPTFYACHLAVLGVLAL"
     gene            28557..29117
                     /locus_tag="ACHJ8C_001234"
     CDS             28557..29117
                     /locus_tag="ACHJ8C_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO5551127.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLGAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLAKIRRQNRRKAELQAVLDEQ"
     gene            29258..29470
                     /locus_tag="ACHJ8C_001235"
     CDS             29258..29470
                     /locus_tag="ACHJ8C_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5551128.1"
                     /translation="MNGFRNSSLNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAY
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            29716..30177
                     /locus_tag="ACHJ8C_001236"
                     /pseudo
     CDS             29716..30177
                     /locus_tag="ACHJ8C_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            30223..30432
                     /gene="hha"
                     /locus_tag="ACHJ8C_001237"
     CDS             30223..30432
                     /gene="hha"
                     /locus_tag="ACHJ8C_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO5551129.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            30471..31061
                     /locus_tag="ACHJ8C_001238"
     CDS             30471..31061
                     /locus_tag="ACHJ8C_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFO5551130.1"
                     /translation="MKLMIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGECWQREKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            31301..31549
                     /locus_tag="ACHJ8C_001239"
     CDS             31301..31549
                     /locus_tag="ACHJ8C_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO5551131.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
     gene            31792..31866
                     /gene="tap"
                     /locus_tag="ACHJ8C_001240"
     CDS             31792..31866
                     /gene="tap"
                     /locus_tag="ACHJ8C_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO5551132.1"
                     /translation="MPGKVQDFFLCSLLLCNISAGWCG"
     gene            31859..32716
                     /gene="repA"
                     /locus_tag="ACHJ8C_001241"
     CDS             31859..32716
                     /gene="repA"
                     /locus_tag="ACHJ8C_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO5551133.1"
                     /translation="MADLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALKFLAELGLMTYQTEYDPTIGCNIPTDITFTPALFASLDI
                     SEEAVASARRSRVEWENRLRKKQGLDALGMDELIARAWRFVRERFRSYQAELKSRGMK
                     RARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRNNN
                     YTRLATASP"
     gene            33631..33915
                     /locus_tag="ACHJ8C_001242"
     CDS             33631..33915
                     /locus_tag="ACHJ8C_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO5551134.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDTAAEILHKSRTDFILEMA
                     CKAAEDIILDRRVFNFNDRQYEEFIEMLDAPVADETAIEKLLARKPQWEV"
     gene            33903..34391
                     /locus_tag="ACHJ8C_001243"
     CDS             33903..34391
                     /locus_tag="ACHJ8C_001243"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5551135.1"
                     /translation="MGGVTAPEPLSSFHQAAEFVSGEAVLDDWLKQKGLKNQALGATR
                     TFVVCRKGSQQIVGFYSLATGSVNHTEAAGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVCRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKSQEQTLFLKLP
                     GK"
     gene            35374..35997
                     /locus_tag="ACHJ8C_001244"
     CDS             35374..35997
                     /locus_tag="ACHJ8C_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO5551136.1"
                     /translation="MARNQEKKTPVIVVKKRRVISPTPVADKTETVIPSVPEEPVPVA
                     DAGRNLTTPIPDGAQAPAASGKKKKGRPPRRPLRAHWTREFTDQCMKKVKVLFPHLRA
                     EDGGFLPLKVGISRDIPAWLAEHPEAGLTRDEWDCAASCITSRRVYLLRTSVTGVTRY
                     DLDGKPAGQVSEDEAQNARRWLAIRERRWQERQATLTTDGSAEPVAE"
     gene            complement(36956..37816)
                     /locus_tag="ACHJ8C_001245"
     CDS             complement(36956..37816)
                     /locus_tag="ACHJ8C_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5551137.1"
                     /translation="MKITDHKLPEGIALTLRMPEGNQKPPLIILCHGFCGIRNVLLPS
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQTEDIISVISWAEQQTDIDNQRIG
                     LWGTSLGGCHVFSAAAQDQRVKCLVSQLAFADGDTLVTGGMSEPERTSFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKEFFGKVKIRHPGMDIKIPFLTVMETLQYKPAESAA
                     RVQCPVLVVIAGRDSVNPPEQGKALYDAVASADKTLYEEAAARHYDLYEGEFFERVVA
                     VQTQWFKKHL"
     gene            complement(38756..39953)
                     /locus_tag="ACHJ8C_001246"
                     /pseudo
     CDS             complement(38756..39953)
                     /locus_tag="ACHJ8C_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(39904..40317)
                     /locus_tag="ACHJ8C_001247"
     CDS             complement(39904..40317)
                     /locus_tag="ACHJ8C_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5551138.1"
                     /translation="MARSAKPRKRKPAPQRSKLSRYVVTLHDDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDVGNKKLVFWFVRVDGEGYPEIARCTEREFATMLAGISAGG
                     MYCPECGTVHWPDGVTRPSDASPFCRYFSAGKPLA"
     gene            complement(40405..40623)
                     /locus_tag="ACHJ8C_001248"
                     /pseudo
     CDS             complement(40405..40623)
                     /locus_tag="ACHJ8C_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045099.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F7-2 fimbrial protein"
BASE COUNT        10234 a         9690 c        11101 g         9628 t
ORIGIN      
        1 gaaattactg gttaaggaga ttaacttcgt aagacaaaca gcacctgccg gacagttccc
       61 ggtcagtgaa tgctggtttt cttcctctga ttcttcaggc agatcagaaa tacaggatat
      121 ctgccattac cgcgcggctg atgctgcaga ttatgtcagg gagactgacc ggtcactgat
      181 gcaactggtc accgcattat gggcaacact ggcactgatg tatgtctcgc tggcagcgat
      241 caccgggaaa tatccggtgc tgcccggaaa aatgaaatgt atccgggtgg taaccgcaga
      301 tgaatatctg aaggacgttt atacggaaga cgcttccctg ccgggagaaa tcaggagatg
      361 ccatgtttat ttccctgatg accgaacaaa cagaagcaac ggagataaaa atgaacatgc
      421 gtaatattaa tgttattaca gcccgcgttg tgccggataa aaacgtatca gatgatttta
      481 tgcatgctgt tctcagtaac tgcaccacaa aaatagttct tcctgctccg aaggagttca
      541 gttctgaatc attgccgcac aattttaata tggctgctgt cggtgtgatg aagaagggaa
      601 tgtcgatgag cttattttct tctccgggaa atacagtggt ctccggacat ccctgtgcag
      661 gggaaacaaa tctgacagca gagttaattt ctgctgttgt ggggagtaat aaaaaaggag
      721 aagggcaatg aaacagattt ctttatttat tcctctgctg ggaaccttat tactttccgg
      781 ctgtgccgga accagtacgg aatttgagtg taacgccacc acatccgata cctgtatgac
      841 gatggagcag gccaatgaga aggccaaaaa actggagcag tcctcagaag caaagccggt
      901 tgcggcatca ctgccgcgcc tggctgaagg gaacttccgg acaatgccgg tgcagaccat
      961 caccgcgacc actccgtccg gcagcagacc tgcggtgacg acacttccgg agcagaaact
     1021 gctgacacct cgcccgctgt ttactgcagc ccgggaagtg aaaacggttg tcccggtcag
     1081 ttcagttgcg ccggtaacgc ctccccgtcc gttaagaacg ggtgagcaga cggcagcatt
     1141 atggatagca ccatatattg ataatcagga tgtttatcat cagccatcca gtgtgttttt
     1201 tgttattaaa ccgtctgcgt ggggaaaacc acgtattaat taacttgccc ggaagttgta
     1261 tatcagccgg caatgccggc gggctttctg tattcaaaat ctgcctgtaa aacgagtgcc
     1321 ctgtgcgtga aaagggatgg gcggtagcgt gtcagaacat gtcgggagga atattccgtg
     1381 agtgatgaag ccgatgaagc atattcagtg acagaacaac tgaccatgac aggaataaac
     1441 cggatacgcc agaaaataaa tgttcatggt attcctgttt atctttgtga agcatgcgga
     1501 aatcctattc cggaagcccg gcggaaaata tttcccggtg tgacgttgtg cgttgaatgt
     1561 caggcgtatc aggaaaggca gaggaaacac tatgcataaa tcagaaaatg aaaaaataga
     1621 ttttattctg gatgagcatg agcggatatc cagaaaacag ggttctttgt gtatggcgct
     1681 gggtaagggc tttttagtta tagctattct ttgtggtgtt gccacattct gtgtttctgg
     1741 cctgtatctg aaatccctgt gcctgtccgt tgcctgctgt tgtggggtat tcagtcgtat
     1801 ttttaattat tacagcgtac cggaggaatc acgcatggtc ctcagccgtc agagcctgct
     1861 tcagcttatg cgtctgactg aagacgtacc ggatgcgaga caggaactgc ttaaccgttt
     1921 gttgtccggt aaaaaactga ctggtcggga tgaaaaggat atccggcgtc tctggcagga
     1981 gaaagtggat gcaatgcagg agtctgccac ccgtcagcgt gaacaggata ccatccggaa
     2041 gtttactgaa gagaataaat ccgaatgaga atctgcagtg ttttataacc tcataagtta
     2101 actgaaggga tattcagtga ttaatattta acaggcggta ataaagaatg cataccttta
     2161 tttttcttgt gctgataaaa aatattaacg gtggcggaag ctggaatgtt actcccatgc
     2221 caaatacgga cgtatgcgtg caggttcttc ggacaatcga agcacacgga ggcaggggct
     2281 ttggaaatac aggggaatgt attgaagtta aaacaaatga acctttgaac aacgtgatgg
     2341 aaacactgaa tggtggtggg caatgagtgc ttactgcttc gaaggaaagt gaaatgaagt
     2401 cgatatattt aaaatctgtt ctggctttta tatttgttgg cgttatggct atgttgatat
     2461 gtggtctttt ttacaatgac taccttgagc aacaacctgc aacaccagaa caactgacag
     2521 agattacaca ggatatcccc tgtgcagcag aagcatttaa ggaagctatt aagtctgaca
     2581 catctgatta tcagcctgaa cctttaagtc ttggtaaggc aaaagagctt gcatcagcgt
     2641 gtagagaaag aaacgaaatg gcagaggtga aacgggtaag agaaaatgag cgcaataaga
     2701 ttcgcgaaaa acaaattcag gcattaaatg atgctcattc agtaaaggaa cgttaatcct
     2761 ggtttcctgt taaaagcctc catctcttat ttgtgctgtg gtttcaggaa tggcactgcg
     2821 tgatatatcc acggccacag actgagattt tcctttttta aacgcactaa ggaatactca
     2881 tatgagtaat aacccacttg aggccgtcac tcaggcggtt aactccctcg tcacagcact
     2941 gaaactgcct gacgaatcag caaaggccaa tgaagtgctg ggcgaaatga gcttcccgca
     3001 gttcagccgt ctgctgccgt atcgtgatta caaccaggaa tccggtctgt tcatgaatga
     3061 caccacgatg ggctttatgt tggaagccat tcccatcaat ggggcgaatg agtccattgt
     3121 ggaggccctc gatcacatgc tgcgcaccaa actgccgcgc ggtattccgt tgtgtatcca
     3181 tctgatgtcc agtcaactgg ttggtgacag gattgaatac gggctgcgtg agttctcctg
     3241 gtcaggtgaa caggctgaac ggtttaacgc cattacccgg gcctattata tgaaagcggc
     3301 agcgacacag tttccgctgc cggaggggat gaatctgccc ctgaccctgc gccattaccg
     3361 ggtgtttatc tcgtactgtt ctccttcgaa gaaaaaaagc cgggccgaca ttctggaaat
     3421 ggaaaacctg gtgaaaatca tccgggcgtc gttacagggg gccagtatca ccacacagac
     3481 ggtggatgca caggccttta tcgatattgt cggggagatg attaaccata acccggactc
     3541 cctgtacccg aaaagacgtc agctggaccc gtattctgat ctgaattatc agtgtgtgga
     3601 ggacagtttt gatctgaaag tccgggctga ttacctgacg ctgggcctgc gtgagaacgg
     3661 caggaacagc acggcccgca tcctgaattt ccatctggcc cgtaacccgg aaatcgcctt
     3721 cctgtggaac gtggccgaca actacagcaa cctgctgaac ccggaactgt ccatctcctg
     3781 tccgttcatc ctgacgctga ctctggtggt ggaagaccag gtgaaaaccc acagcgaagc
     3841 caacctgaag tacatggact tggagaaaaa gtcgaagacc tcctatgcca aatggtttcc
     3901 gtccgtggag aaagaggcga aggagtgggg ggaactgcgt cagcggctgg gctccggtca
     3961 gtcctccgtg gtgtcctact tcctcaacat cacagcattc tgcaaggaca ataatgaaac
     4021 ggcgctggaa gtggagcagg acatcctgaa cagctttcgt aaaaacggtt ttgagctgat
     4081 ttcaccgcgc tttaaccaca tgcgcaattt cctgacctgc ctgcccttta tggccgggaa
     4141 agggctgttt aaacagctga aagaggccgg agtggttcag cgcgcagaga gctttaatgt
     4201 tgccaacctg atgccgttag tggcggataa ccccctgaca ccggcaggtc tgctggcacc
     4261 cacctaccgt aaccagctgg cgtttatcga tattttcttc cgggggatga ataacaccaa
     4321 ctacaacatg gcggtctgtg gcacctccgg ggccggtaaa accgggctga tacagccact
     4381 tatccgcagc gtgctggact ccggaggctt tgccgtggtg ttcgacatgg gggatggata
     4441 caagtccctg tgtgagaaca tgggcggggt gtatctggac ggtgaaaccc tgcgttttaa
     4501 cccgtttgcg aacatcaccg atattgacca gtcagcggag cgtgtccgtg accagttgtc
     4561 agtgatggcc agccccaacg gtaacctgga tgaagtgcat gaaggtctgc tgttgcaggc
     4621 ggtcagagcc tcctggctgg ccaaagagaa cagagcacgt attgatgacg ttgtggattt
     4681 cctgaaaaac gccagtgaca gcgagcagta tgccgagtca ccgactatcc gcagccgtct
     4741 ggacgaaatg attgtgctgc ttgaccagta cactgccaac ggcacttacg gccagtattt
     4801 taactctgat gagccgtccc tgcgggatga cgcaaaaatg gtggtgctgg agctgggcgg
     4861 actggaagac cgtccgtcac tgctggttgc ggtgatgttc tccctgatta tctacatcga
     4921 gaacaggatg taccgcacgc cgcgtaacct caagaaactg aacgttattg atgaaggctg
     4981 gcgtctgctg gacttcaaaa atcacaaggt cggtgaattt attgagaaag gctaccgtac
     5041 ggcccgccgt cataccggtg cctatatcac catcacacag aacatcgtcg actttgactc
     5101 tgacaaggcc tccagtgctg cccgcgcggc atggggtaac tcctcctaca aaattatcct
     5161 caaacagagt gcgaaggagt tcgcgaaata caaccaactg tatccggacc agttcctgcc
     5221 actgcagcgc gacatgattg gtaagtttgg tgcagccaaa gaccagtggt tcagttcctt
     5281 cctgctgcag gtggaaaacc actcctcctg gcaccgtctg tttgtggacc cgttaagccg
     5341 cgccatgtac agctctgacg gcccggattt tgagtttgtg cagcagaagc gtaaagaggg
     5401 gctgagtatt catgaggcgg tgtggcagct ggcgtggaag aagtcagggc cggagatggc
     5461 ttcgctggaa gcctggctgg aagaacatga gaaatacagg agtgttgcat gaccacaacg
     5521 caaaaaacga ctgatgtgac agcccgtcga cgcagccact ggtggtggac ggtgccggga
     5581 tgcctggcaa tggtgttact gaacgcagcc gtcagttacg gcattgtcag actgaacgca
     5641 ccggtgactg tcgccttcaa catgaagcag accgtggatg cgttttttga cagcgccaat
     5701 cagaaacaac tgtcagaagc acagtcaaaa gccctttcag cgcgttttaa tacggcactg
     5761 gaagccagcc tgcaggcgtg gcagcagaaa caccacgcgg tcattctggt gtcgcctgcc
     5821 gtggtacagg gggcaccgga tatcacccgg gaaatccagc aggatatcgc ccggcgaatg
     5881 agggcggaac catgagatgc cgggggctga ttgccctgct gatatggggg cagagtgtgg
     5941 ccgccgccga tcttggtacc tggggcgatc tgtggccggt aaaggagacg gacatgctga
     6001 cggtgatcat gcagcgcctg acggcactgg agcagtccgg tgagatgggc cggaaaatgg
     6061 atgcctttaa ggagcgggtg atccgcaaca gcctgcggcc ccctgccgtg cccggtatcg
     6121 gacgcacgga gaaatacggc agccggttgt ttgacccgtc cgtcagactg gctgcggata
     6181 tccgggataa cgaagggcgg gtgtttgccc gtcagggtga agtgatgaat ccgctgcagt
     6241 atgttccttt taaccagacg ctgtatttca tcaatggcga tgatccggcg caggtggcct
     6301 ggatgaaacg ccagacgccg cccacactgg agagcaaaat tatcctcgtg cagggcagta
     6361 tcccggagat gcagaagtct ctggacagcc gtgtttactt tgaccagaac ggtgtgctct
     6421 gccagcgcct cggcattgat caggtcccgg cacgggtgag cgccgttccc ggcgatcgct
     6481 tcctgaaggt ggaatttatt ccggcagagg agggcagaaa atgaagcgaa gcctgtggct
     6541 gctgatgtta ttccttctcg ccggtcatgt ccctgcagcg tctgcggatt ctgcctgtga
     6601 ggggcgtttt gtaaacccga tcacggatat ctgctggagc tgtattttcc cgctctcgct
     6661 gggcagtatc aaagtcagtc agggtaaggt ccccgacacg gcgaacccgt cgatgcccat
     6721 tcagatttgt ccggcaccgc cgccgctgtt caggcgtatc gggctggcca ttggttactg
     6781 ggagccgatg gcgctgacgg acgtcacccg atcaccggga tgcatggtta acctgggctt
     6841 cagcctgccg gcttttggta aaacggcaca gggaacggcg aaaaaggatg agaagcaggt
     6901 gaacggggcg ttctatcacg ttcactggta caaatacccg ctgacgtact ggctgaacat
     6961 catcacatcg ctgggctgtc tggaaggtgg tgacctggat atcgcttatc tttctgaaat
     7021 tgaccccacc tggacggaca gcagcctgac caccattctc aatccggaag ctgtcatctt
     7081 tgccaatccg atagcacagg ggacctgtgc agcagatgcc attgccagcg cctttaatat
     7141 gcctctcgat gctctgttct ggtgtgccgg ttcgcagggc agtatgtacc cgttcaatgg
     7201 ctgggtgagt aatgagtcca gtccgttgca gtcctccctg ctggtcagtg aacgcatggc
     7261 gttcaagctg caccgtcagg gcatgattat ggaaaccatc gggaaaaata acgccgtctg
     7321 taatgaatat ccgtccccaa tcctgcccaa agaacgctgg cgttaccaga tggtgaatat
     7381 gtacccggac agcggacagt gccacccgtt cgggcgcagt gtgacccgct gggaaaccgg
     7441 gaaaaatccg cccaatacaa agaaaaactt cggctacctg atgtggcgta aacgtaactg
     7501 tgtcttcctg tgaggtgaat gatgaagctg agtatgaaat ctctggcagc actgctgatg
     7561 atgctgaacg gggcggttat ggcgtcagaa aacgtgaaca ctcctgaaaa ccgccagttc
     7621 ctgaagcagc aggaaaattt aagcagacaa ctgcgtgaaa aacctgacca tcagctgaaa
     7681 gcctggacag agaagcaggt gctggaaaac ccccttcagc gttcagataa ccatttcctg
     7741 gatgagctgg ttcgtaaaca gcaggcttcg caagacggga aaccccggca gggtgctctg
     7801 tattttgtgt cgttttccat tcccgaagag gggctgaaac gaatgctggg cgaaacccgg
     7861 tactacggta tacccgccac actgcggggc atggtgaaca atgacctgaa gaccaccgct
     7921 gaagccgtgc tgtccctggt gaaagacggc gcgactgatg gtgttcagat cgacccgacg
     7981 ctgttttcac agtacggcat tcgcagtgta ccggcactgg tggtgttctg cagtcaggga
     8041 tacgacatca tccggggaaa cctgcgggtc ggccaggcgc tggagaaagt ggccgccacg
     8101 ggtgactgcc ggcaggtggc ccgggcgttg ctgaataacc ccggagataa acaaaaatga
     8161 ctttacctgc cgggtattat cggattgatc cggagatccg tgcactggtg gctgcgatga
     8221 acattcacgg cttccgtaca tatgcttcct gtcaggggca tggtttccct gttaccaagc
     8281 tgcttcctta tattgccttt gtctgcccgg taaaaatggc tgcccttctt gagcagcgac
     8341 tgcggcagga tgctgaatca gcaataccac gtctgacatg gggatggtcg gtgaaggggg
     8401 cttttaacag tgagtttcag ctctgtttca ggcttcagcc cgatgcccct cattactggt
     8461 ataaccggta ttgtcgtcac tcgttatgtg ccgatttcag aacactgata tcgttactga
     8521 agtcgttatc tgagtagtaa tcccggtacg tcaagaagcc ccatgtacca taatccgcca
     8581 gcaataataa tagccgtaat aaacagccct gcaaaccact ggtaaaagcg atcgctgcgc
     8641 cgtttttttc caaaagggtc cgttgagctg caattcccgc acgttgtggc tgttcgactt
     8701 aatgctttac cacaatcaac acagttctga agttgagatg acatcacttc attctccagt
     8761 atggttcagg ttatgaaaga aaagataaat acctttttca gatctccgaa ttttaattat
     8821 tccgtaaagc cgtgcaatct ttgtgcaatt gtttttttcc tgagcttttc tgtgtttgct
     8881 gacagcaaca gtgattaccg ggctggttct gattttgctc accagatcaa aggacaggga
     8941 agcagcagta ttcagggctt taagccacag gagagtatcc ccggctataa tgcgaatccg
     9001 gacgagacaa aatattacgg tggcgtgact gccggcgggg atggcggcct gaaaaatgac
     9061 ggcaccacgg aatgggcgac cggtgaaacc ggaaaaacca tcacagagtc attcatgaac
     9121 aagccgaaag acattctttc accggatgca ccgtttatcc agaccggcag ggatgtggta
     9181 aaccgggctg acagcattgt gggaaacacc gggcagcagt gcagtgcgca ggagattagc
     9241 cggagtgaat acacgaatta cacctgtgag cgggatttac aggtggagca gtactgcaca
     9301 cgaactgccc gtatggagct gcaggggagt accacatggg aaacccggac gctggagtat
     9361 gaaatgagtc agctacctgc acgtgaagtg aatggtcagt atgttgtctc cattacctct
     9421 cccgttaccg gtgaaattgt cgatgcgcat tacagctgga gtcgtactta cctgcagaag
     9481 agtgtaccta tgacaattac agtactggga accccactga gctggaatgc caaatactca
     9541 gcagatgcct catttacacc agtacagaaa acactgactg ccggtgttac ttttacgtca
     9601 tctcaccccg tccgggtcgg gaatacaaaa ttcagacgtc atacggcaat gaagctgcgt
     9661 ctggttgtca gggtaaaaaa agcctcgtac accccgtatg ttgtctggtc tgaaagctgc
     9721 ccgttcagca aggagctggg gaaactgaca aaaacagaat gtacggaggc tggcgggaac
     9781 aggacgctgg tgaaagacgg tcagtcatac agcatgtacc agagctgctg ggcataccgg
     9841 gacacatatg tcacacagtc agcagacaag ggaacctgtc agacctacac cgacaatcct
     9901 gcctgtaccc tggtgtcaca ccagtgcgcc ttttactccg aagaaggtgc ctgtctgcat
     9961 gaatatgcca cctactcctg cgagtcaaaa acatccggga aagtgatggt ctgtggggga
    10021 gacgtcttct gtctcgatgg cgaatgcgat aaggctcaga gcgggaagag taatgatttt
    10081 gctgaagctg tttctcagct ggcagcactg gccgctgcag gtaaggatgt tgcggcgttg
    10141 aacggggtgg atgtccgtgc tttcaccggt caggcgaaat tttgtaagaa ggcagcagcc
    10201 ggatacagca actgctgtaa ggacagcggc tggggacagg atatcgggct ggccaaatgc
    10261 agcagtgatg aaaaagccct ggctaaagca aaatcaaaca aactgaccgt cagcgtcggg
    10321 gagttctgtt cgaagaaagt gctgggcgtc tgtctggaga aaaaacgcag ctactgtcag
    10381 tttgattcga agctggcgca gatagtccag cagcaggggc gcaacggtca gctgcgtatc
    10441 agttttggca gtgcgaagca tcctgactgt cgggggatta cggttgatga gctgcagaaa
    10501 attcagttcg acagactgga tttcactaac ttctacgaag acctgatgaa taaccagaaa
    10561 atcccggaca gtggtgttct gacgcagaaa gtgaaagagc agattgctga ccagctgaag
    10621 caggcaggac agtaaggtat gggaaataac cggggaaaat aatgaaggtc atattcacgt
    10681 cgaaccgttt tattgatttt cttatccggc ttttgattac agcgattgtc atttccccgg
    10741 taattatctg gtcgtgggat acggtaaaag aaaccactgt aggcgggatg cttgctgctg
    10801 ctttcgttat tttttactcc ggtgtgctcc tgtttattct gtatttttgt ttttctgtcc
    10861 tgactgacct gcagaaacct gatgaaagaa aaagtgatga aaggaatgaa gatgaataaa
    10921 gcattactgc cactgttact ctgctgcttt atttttccgg cgtcagggaa agatgcaggc
    10981 tggcagtggt ataacgagaa aataaatccg aaggaaaaag aaaataaacc agtacctgca
    11041 gccccccgtc aggagccgga tattatgcag aaactggccg cgctgcagac ggcaacaaag
    11101 cgggcgctgt acgaagccat tctgtatccc ggcgtggata attttgtgaa atatttccgt
    11161 ctgcagaatt actggactca gcaggccggg cttttcacca tgagcgccaa aaaagccatg
    11221 ctggcgcatc ccgaactgga ctataacctg cagtacagcc attacaacgg cacggtccgg
    11281 aaccagctgg cggcagacca ggcgcagcag cgacaggcca ttgcgaaact ggctgaacac
    11341 tacggcatca tgttctttta ccgggggcag gaccccatcg acgggcagct ggcgcaggtc
    11401 attaatggct tcagggatac ctatggtctg agtgttattc ctgtttccgt ggatggcgtg
    11461 attaatccgc tgttgccgga ttcccggact gaccaggggc aggcgcagcg cctcggcgtg
    11521 aaatatttcc cggccatgat gctggttgac ccgaaacagg gcagtgttcg cccgttatca
    11581 tacggcttta tttcacagga cgacctggaa aaacagttcc tgaacgtttc tgaagacttt
    11641 aaaccgaatt tttaatcgcg gatttgattt atgagtgaag attatttgaa aatgtttaca
    11701 gatgttgttc tgttaatatt tgtcattatt gccggttatt tcttttctga gcgtaatgtc
    11761 aggaaaatgt ttctcctgag ctcactggtt ttccttgtta ttaatatcgc gtgtttatat
    11821 gtgcttaccg ccagtctctg gtttctgtgt ggtgcaatta tgagtcagag cgcagcactg
    11881 gttgttacaa tggttattgg ctcattaccg aacgttacga gcttcgacag gttcagaaga
    11941 atatttatct gtattatgtt gtcatcggta tggtccggaa tgatgtggtt ttttataagg
    12001 ggacttatga caggctaagt catataaaac ggtcataaaa accatgcaaa ataatgaggt
    12061 tgattactga agaccatatg ataaaccata ccgcaatcca gtggcttctg aatagcttac
    12121 aaaaataaat atagataatt gaaaataacc ataatacaac gatgcctgct gcgatactta
    12181 ataaacttat tacaagatgg tcagggaagg cattgaccgg taatgaatgt gatgctgcat
    12241 atatcagcgc aataattgcc gcatttccca gtaacgactc tcttattatg tttcttatta
    12301 tttccagttt ttctttaaaa gatcgctttt ccatttgtac ctctgattaa tatcacacaa
    12361 acgcgaatat tacagatatg aatactctca tgtaaataat aacaggagtt taacatgata
    12421 agtaaacgca gattctcttt accccggctt gatattacgg gaatgtgggt gttttccctg
    12481 ggtgtctggt ttcatatcgt cgcccgtctt gtttacagca aaccctggat ggcttttttt
    12541 ctggcagagc tgattgcggc catccttgtg ctgttcggcg cataccaggt gctggatgca
    12601 tggattgccc gtgtcagccg ggaagagcgt gaggcactgg aagccaggca acaggccatg
    12661 atggcagagc agcaggaggg cggacatgtc tctcactaaa ttactgccgg tcattttgtt
    12721 gatgatggcc acaggtgtgc aggcctccac ccgggatgaa attgaacgac tctggaatcc
    12781 gcagggtatg gccgcgcagc ctgcacaacc ggcagcagac acgtcagcca ggacagaaaa
    12841 gccggctccc cgctggttcc gcctcagcaa cggcaggcag gttaacctgg ctgactggaa
    12901 ggtggtgctg tttatgcagg ggcattgccc ttactgtcac cagtttgacc cggtactgaa
    12961 acagctggca cagcagtatg gcttttccgt ttttccctac accctggatg gtcaggggga
    13021 taccgccttt ccggaagcat taccggtgcc accggacgtg atgcagacct tcttcccgaa
    13081 tattccggtg gccacaccga ccaccttcct ggtcaacgtc aacacgcttg aggcattacc
    13141 gcttttacag ggggcaacgg atgccgccgg ttttatggcg cggatggata ccgttctgca
    13201 gatgtacgga ggaaaaaaag gtgcgaaata agcagatggt gttattcatt gccggaatat
    13261 ccggcatcgc gacgggaata atcgtcagcc tgaatatccc ctttatccgt caggggcttt
    13321 tttatcccgc cagccctgta gaaattgtcg tttcgctgtg tcttaccttt tctgtttctg
    13381 ttgtgttttt tgtgggggct attgtgggat ggatttcggt gtctgaaata tattacagcc
    13441 gcatgaccgg tctgaatgaa tcatcggaaa tatcataggg aacttataat gagagaaaat
    13501 aaaaataatc ctgaactcaa aatcagaagt acagaacgag attataaata catttccgga
    13561 attactgaca ggtatgctgg attgtctctg gtattcctga ctgccggaat tgttttatgg
    13621 acagttatga atattatttt tgatgcctgt attgattcgt ggaaagcgga cccggaatta
    13681 aataattcga tttatatgtg gaatatcctg atgtatgcca ttccgtgtac gttatgggcg
    13741 ctggcatccg gtttttttgt tgcgggttat ctgctgcctt tatgtgcatt agcagtcaaa
    13801 aatatcatga tattccggtt gaaacgccgt atgcgccggg agaacacatt aagggagggc
    13861 agcaataatg cttcgcatta agcctctttt tattctttgt gctgccttac tgacgctgac
    13921 gcctgcagcg tcagcggatg tgaacagcga catgaatcag ttctttaaca agctgggctt
    13981 cgcctccaat accacacagc cgggcgtctg gcaggggcag gctgccggct atgcttacgg
    14041 cggctccctg tatgcccgta ctcaggtcaa aaatgttcag ctgatttcca tgacgctgcc
    14101 ggatatcaat gccggatgtg gcggcatcga tgcctacctc ggttcgttca gttttattaa
    14161 tggcgagcag ttgcagcgtt ttgttaagca gattatgagc aatgctgccg gttacttttt
    14221 tgaccttgcc ctgcagacaa cggtgccgga aatcaaaacc gcaaaagact tcctgcagaa
    14281 aatggcaagc gacattaaca gtatgaacct cagttcctgc caggcggcac aggggattat
    14341 cggcgggctt ttcccccgga cgcaggtgtc ccagcagaaa gtctgtcagg acattgccgg
    14401 tgagagcaat atttttgctg actgggcggc ttcccggcag ggatgtactg ttggcgggaa
    14461 atctgacagc gtcagagata aagccggcga caaggataag gagcgggtga ccaaaaacat
    14521 taatatcatg tggaatgcgc tttccaaaaa cagaatgttt gacggcaaca aagagctgaa
    14581 agaatttgtg atgacgctga ccggctcact ggtgtttggt cccaacggcg aaatcacacc
    14641 gctgtcagcc agaaccactg accgctcaat tatccgggcc atgatggaag gcggtacagc
    14701 aaaaatttat cactgcaacg attctgataa atgcctgaaa gtggtggcag acacaccggt
    14761 gaccatcagc cgggataatg cactgaagtc tcagattact aaacttctgg ccagcattca
    14821 gaacaaggcg gtcagtgaca cgcctctgga tgacaaggaa aaaggcttta tttccagtac
    14881 caccatcccc gtcttcaaat acctggttga cccgcagatg ctcggtgttt ccaacagcat
    14941 gatttaccag ctgacggact atatcggtta cgacatcctg ctgcagtaca ttcaggagct
    15001 gatacagcag gcccgggcga tggtggccac gggaaattat gacgaagcag ttatcgaaca
    15061 tattaacgac aatatgaatg atgccacccg gcagattgcg gcgtttcagt cacaggtgca
    15121 ggtacagcag gatgcgctgc tggttgtcga tcgtcagatg agctacatgc gtcagcagct
    15181 ttccgcccgc atgctcagtc gttaccagaa caactatcac ttcggaggga gcacgctgtg
    15241 aatgaagttt atgtgattgc cggtggtgaa tggttgcgga ataacctgaa tgccattgcc
    15301 gcctttatgg ggacccggac gtgggattcc attgaaaaaa ttgcgctcac attgtctgtt
    15361 ctcgcggtgg ccgtaatgtg ggtacagcgg cacaacgtga tggatttgct gggctgggtg
    15421 gccgtgtttg tgcttatcag cctgctggtt aatgtccgca catcggtgca gattattgat
    15481 aacagtgacc tggtcaaagt tcaccgggtt gataatgtgc cggtcggtct ggcgatgcca
    15541 ctttcactga cgacccgtat cgggcatgca atggtggcca gttacgagat gatcttcacg
    15601 caaccggaca gtgtcaccta cagcaaaacg gggatgctgt ttggcgcaga actggtatca
    15661 aaaagcaccg actttttgtc ccgcaatccg gaaatcgcca atcttttcca ggattatgtg
    15721 cagaactgcg tgatggggga tatttacctg aaccacaaat acacgctgga ggagctgatg
    15781 gcctccgctg atccctacac gctgattttt tcccgcccca gtccgctgcg gggcgtttat
    15841 gacagcaata ataatttcgt gacctgtaag gacgcgtcgg tttcgctgaa agacaagctg
    15901 aatctggata cacagagtgg cggaaaaacc tggcattatt atgcccagca gctatttggt
    15961 ggacgccctg atccgaacct gttgttcagt acgctgattg gcgacagtta cagttacttc
    16021 tatggctcaa gtaaatcagc cagccagatc atccgccaga atgtcaccat caatgcgctg
    16081 aaggagggga tcaccagtta tgccgcacgt aatggtgatt cagccagcct ggtgaatctg
    16141 gccaccacgt catcgatgga gaagcaacgt ctggcacatg tctccatcgg tcatgtggcg
    16201 atgcggacac tgcccatgac gcagaccatc ctgacgggga tcgccatcgg tattttcccg
    16261 cttctggtac tggcggctgt cttcaacaaa ctgacgctgt ccgtcctgaa aggctatgtg
    16321 tttgccctga tgtggttgca gtcatggcca atgttgtacg ccattctgaa cagcgccatg
    16381 acattttatg cgaagcagaa tggtgcaccg gtggtgctct cggaaatttc acagatacag
    16441 ctgaaatact ccgatctggc ttccactgcc gggtatcttt ccatgatgat cccgccatta
    16501 tcctggatga tggtcagggg actcggggcc ggattttcca gtgtgtacag ccatttcgct
    16561 tcctctgcta tcagcccgac tgccagtgcg gcaggtagtg tagttgacgg taattactcc
    16621 tacggcaaca tgcagacgga aaacgtgaac ggattcagct ggagcaccaa cagcactacg
    16681 tcgtttggtc agatgatgta ccagaccggc agtggcgcaa ccgccacaca gacccgtgac
    16741 ggtaatatgg tgatggatgc gagcggggcg atgtcccgtt taccggtcgg tatcaatgca
    16801 acgcgtcaga ttgcggcggc acaacaggaa atggcccggg aggcgtcgaa cagagcagaa
    16861 agcgccctgc atgggttcag cagcagtatt gccagtgcgt ggaacatgct cagccagttt
    16921 ggctctaacc gggggagcag tgactctgtc accggtggtg ctgacagcac catgagtgca
    16981 caggactcca tgatggccag ccgtatgcgc agtgcagtgg aaagctatgc gaaggcgcat
    17041 aatatcagta atgagcaggc gacaagagaa ctggcatcca gaagtacgag tgtatctgct
    17101 ggattgtatg gtgatgttca tctggagaag aactttaaac cgagctttat gggggttggt
    17161 ggtggtattg gtgtgaaggc tggagttaaa gggagtattg acggtagtga tctggactca
    17221 catgaagcca gtagtggttc gcgggccagc catgatgctc gccatgatat cgatgctaaa
    17281 gccagtaagg actttaaaga ggccagcgat tactttatga atcgcaaggt cagtgaatcc
    17341 ggcagccata ctgacaataa tgccgattcc cgcgtggacc aactgtctgc agccctgaac
    17401 tcagcgaaac agagttacga ccagtacacg acgaatatga ctcgcagcca tgaatatgct
    17461 gaaatggcat cccgtactga gagtatgagt gggcagatga gtgaagacct gtcgcaacag
    17521 tttgcacagt atgtgatgaa atacgcgccg caggatgcag aagctattct gacaaatacc
    17581 agttcaccgg agattgcaga acgtcgtcgg gcgatggcct ggtcttttgt tcaggaacag
    17641 gtgcagcctg gtgttgataa tgcctggcgt gaatcccgtg ggggtattgg taaaggaatg
    17701 gatagcgtac ctttgggagg tggtcgccag gatatcattg ctgattatca gggacatcag
    17761 gccattattg aacaaagaac gcaggacagt aatatccgta acaatgtgaa gcagcaggtt
    17821 gataatatgg taagggatta tcgttcagaa attgatacgg tgaataaaag tattcgcaac
    17881 gatgaaggta gcataagaca gcaatataat gctttgcaga cccatcataa atcagaagaa
    17941 ttatctcagg atcgtaaata taatgaagaa aaagccgccc aggagcgaat gcccggggca
    18001 gacactccgg atgagttaca gaagaaggct agagaatatc tgggtaagaa tagttaaatt
    18061 tatatatctt ctaacactac ggcatcacct gtagctatta tataggttgc aaaataacca
    18121 ttcccagtgt tgtatgagcc ggaaatatat tgaaagttta caatagcgtt agcccccatt
    18181 ttggaggctt cagataacag agccgaaagc agttcatcat cttcattgta atatttccca
    18241 gatatttcag ctgtcgttgc tttaataagg ccaaaatttt taacaatttt catttgagat
    18301 ggagtggaag tgaaaacatt gctatgagca cttatagtga tattggtttt atggtcaaat
    18361 aatcccatga acatctcctg tacaattgat cgtggaaaac gacagtttca tgttttatta
    18421 tacagtatta atggcgaacc tgtaagaaca taacgaggat attgtgatag aaatgaaaaa
    18481 attgatgatg ggtacacaag tcaatgccag tcgcctcttt tatatggcgg taatgaatac
    18541 ggcaatcgag aaatgaaact ttgaggtaag ggggattctt ttatggatat caagaagaat
    18601 ataataattg cttttgctac aactttattg ggcgggtgtg gtagtattgg aaatataaaa
    18661 ggtgtggaac catctaaata cgcacatgtt actgactact taaatgatat atttgaaaaa
    18721 tcaaattgta ttggtacagt tttctccccg cagggacgga tatcaactga tgccgatgga
    18781 ttgaagtatc agttgaatgg ggaaaatatc aggtgttact ctgactctat tgagaatgct
    18841 gtagagaaat attgtgttta taagggagga gaaatagtac agggagggcg ttggtgtaag
    18901 gtaaacggtg aaccattatt ttatattaat tatggatatg atgggaaaca ttatggcgct
    18961 cctaaagaga attttatggt tgtagaacaa gggagttttc agggaaggca acaatggtta
    19021 attgctgacg agcgacttgg ttttttgagt gaacgtgtcc tgttacaaca aaagttagaa
    19081 aatgaaatta atgcaaaaaa aagaagtcgc agagttacaa cgaaaaacaa aagaaaaaaa
    19141 tacacctgtg aatgcggatg ttggggattt gatttgtaaa gaagcttatg aagctaaacc
    19201 atatcaatat cctggaactg cttactataa agcatatgtt gaaaaaaaag aaaaaataaa
    19261 cttcaactta ggttagtttg gcatggtggt aatggtttca ttattaatga tattgcacct
    19321 tcgaataaca ttatatggtc atcaccaaat ggctggaaac attgtgattg atttatgagc
    19381 tgacgcctcg ctttactcgt tgtccaactc cggggagaaa taaaataaat tttatttctc
    19441 cccggagttt ccgaaataaa tatgaaaaca ctgagcggtt gtgcaaattc tttgtgcgac
    19501 agtgaagtgt tgccggcgaa gccggaattt aacggttatt aatcttttca tcattaataa
    19561 tcagtccgac tgactttctt tcttcggaat atcatcatga gttttaacgc aaaggatatg
    19621 acccagggcg gtcagattgc gtccatgcgt atccgcatgt tcagccagat cgccaatatc
    19681 atgctttact gcctgtttat ttttttctgg atactcgttg gtctggtttt atgggtaaaa
    19741 ataagctggc agacgtttgt gaacggctgt atttactggt ggtgtaccac gctggaaggc
    19801 atgcgggatt taatcaagtc ccagccggta tatgagatcc agtattacgg caaaaccttc
    19861 cggatgaacg ctgctcaggt actgcatgat aaatatatga tctggtgcgg agagcagcta
    19921 tggtccgcat tcgttctggc cacagttgtg gcactggtta tttgcctgat taccttcttt
    19981 gttgtctcct ggattctggg gcgtcagggt aaacaacaga gcgaaaatga agtcacaggt
    20041 ggtcgtcagc tgacagacaa tccgaaagac gttgcccgga tgctgaaaaa agacggcaag
    20101 gactccgata tccggattgg cgacctgccg attatccggg attccgaaat ccagaacttc
    20161 tgtctgcacg gaacagtgtc gacgggtaag tcagaagtta tccgacgcct tgccaactat
    20221 gcccgtaaac gcggcgatat ggtggtgatt tatgatcgtt cgtgtgagtt tgtcaaaagt
    20281 tactatgatc cctccatcga taagatcctg aatccgctgg atgcacgctg tgccgcctgg
    20341 gatttatgga aggagtgtct gacacagccg gattttgata atgtcgcgaa cacgctgatc
    20401 ccgatgggca caaaagagga tccgttctgg cagggctccg ggcgcaccat ctttgcggaa
    20461 gcggcgtacc tgatgcgcaa tgatcccaac cgcagctaca gcaaactggt ggatacactg
    20521 ctttccatca aaattgaaaa actgcgtacc tatctgcgta actcaccggc ggctaacctg
    20581 gtggaagaga aaattgagaa aacggcgatt tccatccgtg ctgtgctgac caactacgtg
    20641 aaagccatcc gttacctgca ggggattgag cacagcggtg agtccttcac tattcgtgac
    20701 tggatgcgtg gtgtccggga agataagcaa aacggctggc tgtttatctc gtcgaatgcc
    20761 gacacccatg cctccctgaa gccggtggtt tccatgtggc tgtccattgc cattcgtggg
    20821 ctgctggcaa tgggagaaaa ccgtaaccgt cgtgtgtggt ttttctgtga tgagttaccc
    20881 acgctgcaca aactgccgga tctggtggag atcctgccgg aagcccgtaa gttcggtggc
    20941 tgttatgtgt ttggtatcca gtcctatgcc cagctggaag atatctacgg tgagaaagcg
    21001 gcggccacgc tgtttgatgt gctgaatacc cgagcctttt tccgctcccc gagtcataag
    21061 attgcagagt tcgctgccgg tgaaattggt gaaaaagagc acctgaaagc cagcgagcag
    21121 tattcctacg gtgcagaccc ggtacgtgac ggggtatcaa ccggtaagga gatggagcga
    21181 cagacgctgg tcagttattc cgacattcag tctctgccgg atctgacctg ttatgtcacg
    21241 ctgcccggac cgtatccggc agtaaaactc tcactgaaat atcaggcacg tccgaaggtc
    21301 gctccggagt ttattccgcg tgacatcaac ccggaaatgg agagccgtct gagtgccgta
    21361 cttgccgcaa gggaagcaga aggtcgtcag atggccagcc tcttcgaacc ggatgtcacg
    21421 gaggttgttt ccggagaaga cgtgactcag gctgaacagc cgcaacagcc ggtgtctcct
    21481 gctatcaacg ataagaagtc tgattcaggt gtgaatgttc cggcaggggg gatcgagcag
    21541 gcgctgaaaa tgaaaccgga agaagagatg gaacagcaac tgccacccgg gatcagtgaa
    21601 tccggtgaag tggtggatat ggccgcttat gaggcatggc aacaggaaaa tcatccggac
    21661 attcagcagc agatgcagcg tcgtgaagag gtgaacatta atgtgcaccg ggagcgcggt
    21721 gaggatgttg agccgggaga tgatttctga tgaacgggtc agctccagtc ttcaattctc
    21781 agaccgtcca cacgttcaaa ttcccgggtg ttgttagtca caactatcag cccccgactg
    21841 cgggcatgac ctgcgatcat ttgatcaaat ggcccgacag ggcgtccctg acgggaaagt
    21901 tctgctctta tctggccggt gtgtgtggct gcagcagcgt cgtaatccag aacatcaatg
    21961 cgggaaacaa atccctcgat cacagcgaga ttgcgctcag gcatctggct tttttctgca
    22021 ccatatatca gctccatcag ggtgacagag ctgatacaca ttctcccctg gttcaggtta
    22081 aaacgttctc tgacactggt gggcttgttc tttatcgtga aaatgcagat gttggtatcg
    22141 agcataaact tcagcatcag aatgactccc tttcctgcat gccgggctgt tccctgttat
    22201 ccataaaatc agcgctgacg ctgttaccgt cgaaccattc atcccatgac tctccggctg
    22261 gcgtaatgat acgggttcgt ccgacagcaa tcacttcaac gcgttttacg ttttctggta
    22321 atgcaaccgc ttttggcagc ctgaccgcct ggctgcggtt gctgagaaat acggtggttt
    22381 ccattatttg cctccttatg cggatatgtc ttgtgtatat ccattgtggg attgataccg
    22441 ggatatgtca aagggatata cgtttatgat gagtatcgcg caggtcagat cggccggaaa
    22501 tgcagggaac tattataccg acaaggataa ttactatgtg ctgggcagca tgggagaacg
    22561 ctgggccggc cagggggctg aacagctggg gctgcagggc agcgtcgata aggatgtttt
    22621 tacccgtctt ctggagggca ggctgcctga cggggcggat ttaagccgca tgcaggatgg
    22681 cagtaataag catcgtcccg gctacgacct gaccttctct gcccccaaaa gtgtctccat
    22741 gatggccatg ctcggggggg acaaacgtct tattgaagca cataaccagg ccgtggattt
    22801 tgctgttcgt caggtggagg cactggcctc cacacgggtg atgacggacg gacagtcaga
    22861 aacggtgctg accggtaatc tggtgatggc actgtttaac cacgacacca gtcgtgatca
    22921 ggaaccacag ttacacacgc atgcggtggt ggctaatgtc acgcagcata gcggcgaatg
    22981 gaagacactg agcagtgaca aagtggggaa aacgggattc attgagaatg tgtacgctaa
    23041 tcagattgcc tttggcaggc tctaccggga aaaactgaaa gagcaggttg aggcgctggg
    23101 ctatgaaacg gaagtggttg gtaagcacgg tatgtgggaa atgccgggcg taccggtgga
    23161 ggccttttcc ggacgctcac agaccatccg ggaggccgtc ggggaagacg cctcgctgaa
    23221 atcccgggat gtggcggcgc tggatacgcg taaatccaaa cagcatgtcg atccggagat
    23281 caaaatggcc gagtggatgc agacgctgag ggaaaccggg ttcgacatcc gggcatatcg
    23341 tgacgcggcg gatcagcggg cggagacccg cacgctgacg cccgggccag cttcaccgga
    23401 cgggccggat gtgcagcagg cggtgacaca ggccattgcc ggattaagtg aacgcaaagt
    23461 gcagttcacg tatacggacg tactggccag gacggtcggc atactgccgc cggaaaatgg
    23521 tgtgattgaa cgggcacgcg ccggtatcga tgaggccatc agccgtgagc agcttatccc
    23581 tctcgaccgt gagaaggggc tgttcacgtc cgggattcat gtgctcgatg agctgtcagt
    23641 ccgggcactc agtcgtgaca tcatgaaaca gaaccgggtg accgtacatc cggagaaaag
    23701 tgtcccccgg acggccggtt acagcgatgc cgtcagtgtt ctggcacagg atcgcccgtc
    23761 gctggccatt gtgtccgggc agggcggtgc agccgggcag cgtgagcggg tggctgaact
    23821 ggtcatgatg gcccgggagc aggggcggga ggtgcagatt atcgctgctg accgtcgttc
    23881 gcagatgaac ctgaaacagg atgaacggct gtccggtgag ctgataaccg gacgtcgtca
    23941 gctgctggaa ggcatggcct tcacgccggg cagtactgtt atcgttgacc agggcgaaaa
    24001 actctccctg aaagagacgt taaccctgct ggatggtgcc gcacgtcata acgtacaggt
    24061 cctgataacc gacagcgggc agcgaaccgg tacaggcagt gcactgatgg ccatgagaga
    24121 tgccggggtg aacacatatc gctggcaggg gggagagcag cgaccggcaa ccattatcag
    24181 tgaaccggac cgtaatgtcc gctatgcccg gctggccgga gattttgcgg ccagcgtgaa
    24241 agccggagaa gagagcgtgg cacaggtcag cggggtacgg gaacaggcca tactgacaca
    24301 ggccattcgc agtgaactga aaacacaggg cgtgctcgga cacccggagg taaccatgac
    24361 cgccctgtca ccggtctggc tggacagccg gagccgttat ctgcgggata tgtaccgtcc
    24421 ggggatggtg atggagcagt ggaacccgga gacacgccgt catgaccgct atgttatcga
    24481 ccgggtgacg gcgcagagtc acagcctgac cctgcggaat gcacagggtg aaacgcaggt
    24541 ggtgcgtatt tcctcgctgg acagcagctg gtcgctgttc cggccggaaa aaatgccggt
    24601 ggcagacggc gagcgtctga gggtgacagg gaaaattccc ggactccgcg tctccggcgg
    24661 tgaccgcctg caggtggcat ccgtcagtga agatgcgatg acggttgttg tgccggggcg
    24721 ggctgaaccg gcctccctgc ctgtgagcga ttcaccgttc acggcactga agctggagaa
    24781 cggctgggtg gaaacgcccg ggcattccgt cagcgacagt gcgacggttt ttgcctccgt
    24841 cacacagatg gtgatggaca acgcgaccct gaacggtctg gcccgcagtg gtcgtgatgt
    24901 ccggctgtat tcctcactgg atgaaacccg tactgcggaa aaacttgccc gccatccctc
    24961 ctttacggtg gtttctgagc agataaaggc gcgtgccggt gagacattgc tggaaaccgc
    25021 tatcagtctg cagaaagcca ggcttcacac gccggcacag caggccattc atctggccct
    25081 tcctgtgctg gaaagtaaaa acctggcctt cagcatggtg gacctgctga cagaggcgaa
    25141 gtcgtttgct gcagaaggaa ccggttttac tgaactggga ggggaaatca atgcgcagat
    25201 aaaacgcggt gacttactgt atgtggatgt ggcaaaaggc tatggcacag gcctgctggt
    25261 ttcccgtgcg tcgtatgagg cagaaaagag cattcttcgc catattctcg aaggtaagga
    25321 ggcggtcacg ccgctgatgg agagagtacc tggcgaactc atggagaaac tgacatcagg
    25381 acagcgtgcc gccacccgca tgatactgga aacgtccgac cgtttcacgg tggtgcaggg
    25441 ctatgccggt gtgggtaaga ccacacagtt ccgggcggtg atgtcagccg tgaacatgct
    25501 gccggagagt gagcgtcccc gtgtcgttgg gctgggtccc acgcaccgcg cggtcgggga
    25561 gatgcgcagc gcaggcgtag atgcacagac actggcgtcc tttctgcatg acacgcagct
    25621 gcagcagcgc agcggagaaa cgccggattt cagcaacacg ctgttcctgc tcgatgagag
    25681 ctcaatggtg ggcaataccg acatggcacg ggcatacgcc ctgattgcgg ccggtggcgg
    25741 tcgggctgta gccagcggtg acacggacca gctgcaggcc atcgcgcccg gtcagccttt
    25801 ccgtctccag cagacgcgca gtgccgccga tgtggtcatc atgaaggaga ttgtgcgtca
    25861 gacgccggaa ttgcgggagg cggtatacag cctgattaac cgggatgtgg aaagggcact
    25921 gtccgggctt gagagtgtga aaccgtctca ggtgccacgt caggagggcg catgggcacc
    25981 ggagcactcc gtgacggagt tcagtcacaa ccaggaagcg aaactggcag aagcgcagca
    26041 gaaggcgatg ctgaaaggcg aggctttccc ggatgtcccc atgacactgt atgaagccat
    26101 tgtccgcgac tataccggca ggacaccgga agcacgggag cagacgctga ttgttacgca
    26161 cctgaatgag gaccggcgcg tactgaacag catgattcat gatgcacggg aaaaggccgg
    26221 tgagctgggg aaagagcagg ttatggtgcc tgtcctgaac acagcgaata tacgcgacgg
    26281 ggaactgcgt cgtctttcca cctgggagac acatcaggac gcacttgccc tggtggataa
    26341 tgtgtatcac tggattgccg gtatcagcaa ggatgacggg ctgataaccc tgcaggatgc
    26401 ggaaggtaac acgcggctga tttcgccccg ggaggcggtg gctgaaggcg tcacactgta
    26461 cactccggac accatccggg tgggaaccgg tgaccggatg cgcttcacga agagtgaccg
    26521 ggagctcggt tatgtggcca acagcgtctg gacggtgaca gcggtttccg gtgacagtgt
    26581 cacgctgtcg gacggacagc agacccggat gattcgcccc gcccgggagc gggcagagca
    26641 acatattgac ctggcctatg ccatcaccgc ccacggtgcg cagggggcga gtgaaacctt
    26701 tgccattgcg cttgaaggca cggaaggtaa ccggaaactg atggccggct ttgagtcagc
    26761 ctacgtggcc ctgtcgcgta tgaagcagca tgtgcaggtg tacaccgata accgccaggg
    26821 ctggacggat gccattaaca atgccgtaca gaaaggaact gcccacgatg tgtttgagcc
    26881 gaaaccggac cgggaggtca tgaatgcaga gcggctgttc agtacggcgc gggagctgcg
    26941 ggacgtggcg gcagggcgtg ccgttctccg tcaggcgggg cttgccgggg gagacagtcc
    27001 tgcacggttt attgctccgg ggcgcaaata cccacagccg tatgtggcac tgccggcttt
    27061 tgaccgtaac ggcaagtctg ccggtatctg gctgaacccg ctgaccacgg atgacggaaa
    27121 tgggctgcgg ggattcagcg gtgaaggccg gataaaaggc agcgaagatg cgcagttcgt
    27181 ggccctgcag ggcagccgta acggagaaag cctgctggct gataatatgc aggatggtgt
    27241 ccggattgcc cgtgataatc ctgacagcgg tgtggtggta agaatcgccg gtgaaggtcg
    27301 cccgtggaat cccggagcca tcaccggtgg ccgcgtgtgg ggggatatcc cggacaacag
    27361 cgtccagccg ggtgccggaa atggcgaacc gctcacggca gaggtactgg cacagcggca
    27421 ggctgaagag gccatccgcc gtgaaacgga acgccgcgca gatgaaattg tccgtaaaat
    27481 ggcagagaac aaacctgacc tgccggatgg caaaacagag ctggctgtca gggaaattgc
    27541 cgggcaggag cgtgaccggg ctgccataac tgaacgggaa gccgcgctgc cggagagtgt
    27601 actgcgtgaa ccacaacggg aacgggaagc ggtccgcgag gttgcccggg aaaacctgct
    27661 gcaggagcgt ctgcagcaga tggagcggga tatggttcgt gacctgcaga aagagaaaac
    27721 cctgggcgga gactgataca ggaagacaaa cactgatgac aacggataac acgaacacga
    27781 cacgtaacga ttcactggct gcccggaccg atacctggtt gcagtcgttg ctggtctggt
    27841 cccccggaca gcgggatatc atcaaaacgg tggcactggt gctgatggta ctggaccatg
    27901 caaaccgcat tctgcatctg gaccagacgt ggatgtttct ggccgggcgt ggagccttcc
    27961 cgctttttgc cctggtatgg gggatgaatc tgtcctgtca tgcgcatatc cggcaaccgg
    28021 ccattaaccg gctgtgggaa tggggaatta ttgcccagtt cgcgtattat ctggccggct
    28081 ttccctggta tgaggggaat atcctgtttg cctttgcagt ggcggcacag gtgctgacat
    28141 ggtgtgaaac gcgcagtggg tggcgttccg cagcggccat tctcctgatg gcactgtggg
    28201 ggcctttgtc cggcaccagt tatagcattg ccgggttgct gatgctggca gtcagccgca
    28261 ggctgtaccg ggcggaggac agaacggaac gtctggcgct ggttgcatgc ctgctggccg
    28321 tgattccggc gcttaacctt gccaccagtg atgcggcggc tgtagccggt cttgttatga
    28381 cggtgctgac tgttggtctg gtgtcctgta cagggaaatc actaccccgt ttctggtacg
    28441 gggatttttt cccgacgttc tatgcctgtc atctggctgt gctgggcgtt ctggcgctgt
    28501 gacgggtgtg gtatctttga ctgtaacagg atgattcgtc agaggcagag cgcagcatga
    28561 cagagcagaa acgaccggta ctgacactga agcggaaaac ggaaggggag acgcctgtcc
    28621 gcagccggaa aaccatcatc aatgtcacca cgccaccaaa atggaaggtg aaaaagcaga
    28681 aactggccga gaaggcggcc cgggaagcag agctggcggc aaaaaaagcg caggccagac
    28741 aggcgctgtc catttatctg aacctgccca cgctggatga ggctgtgaac accctgaagc
    28801 cctggtggcc gggattattt gacggtgaca cgccccggct tctggcctgc ggtatccggg
    28861 acgtgttact ggaagacgtg gcgcagcgga atatcccgct ctcgcataaa aaactgcgac
    28921 gtgcgctgaa ggccatcacc cgttcagaaa gctatcttgg cgccatgaaa gccggtgcct
    28981 gtcggtatga cacggaaggg tatgtaacgg agcatatttc tcaggaggag gaagcgtatg
    29041 cggcagcgcg tctggcaaaa attcgtcgtc agaaccggag aaaagccgaa cttcaggctg
    29101 tgctggatga acaataaaaa aagcctccct caaagcaggg aggcagaaca ataaggctta
    29161 aaaggaaata acttccgtat ttgtaaggaa caaggaagtt ggtccactgg attatttaac
    29221 atagtcataa taattgatta aagggggagc agtaaagatg aatggattca gaaacagttc
    29281 actgaacggt caggtctggc gctggcagcg tgccggaagc cgggccgtta ttctggaggt
    29341 cagtggacgc tggatggaag cggcagaagc atggagacgg gcggcttatg ttgcgccccg
    29401 gacagactgg cagcagtttg cccgaaaaag ggctgaacac tgccaccggc gctgccgggg
    29461 aagggtgtaa cgaaaaaagc agccttcatt acaccagaag tcagaaggct gaaaggagtt
    29521 tatgagattt gcttttttta atatccggtc gggcagtatg accggacagt tctgagtagc
    29581 acagagtttt ttccgtatta agtcgtgatt ttatattttg tgattaatct cacaagataa
    29641 ggtgttattc aatgtgtgtc gcaatattca ggatgcctga aatacaggta ccatttattt
    29701 gatatggaga ataacatgag gaaatatatt tcactggtat tatttatctt ttcatggccg
    29761 gtattgtctg cagatattca tggacaggtt gttcgtgtac tggatggtga taccatcgag
    29821 gtaattgact caatgaaggc ggtgcggatc aggctggtaa atattgatgc accggaaaag
    29881 aaacaggatt atgaccggag gtaaatggac atgatgaaat cactggttgc aggaaaaacg
    29941 gttaccatca catattttca gcgagatcgt tatggtcgta ttcccgggca ggtttatgca
    30001 ccaggcggaa tgaatattaa tcagtttatg gttcgggccg gggctgcctg ggtatatgag
    30061 cagtacaaca cagatcccgt gctgcctgtc cttcataacg aagacagaca acaaaaacgt
    30121 gggctctggt cagatgctga tccggttccc ccctggatat ggaggcaccg taaataaagc
    30181 gatggcaatc ctatcattca tttgttctca gcgggaataa aaatggcaaa aacaaaacag
    30241 gaatggctgt atcagttacg gcgatgttca tcagtcaata cgctggaaaa aattattcat
    30301 aaaaacagag acagtttatc aaacagtgaa cgtgagtcat ttaattcagc agcagaccac
    30361 cggttagcgg aattaatcac cggaaagtta tatgacagga taccaaaaga gatatggaaa
    30421 tatgtaaggt aataattcag caataattaa ttgccaggag cagataaata atgaagttga
    30481 tgatttttat tctcattgtt ctgattattg cagcgttact tatccggatt atcctcagaa
    30541 gtgtaaatca gcattctcct ttgctgatgc aattgcatgc ggcaggtata cggaccggtg
    30601 atgcagagcg aatactgtcc agcggtgaat gctggcaacg tgagaagacg ctgctgacag
    30661 aaagggaagt cagttttatg aaaggactgt tcagaattgt ggatatgaag cggtggtatc
    30721 tgtgtccgca ggtacgggtc gcggatatcg tccagctgaa cgggaatatc cggccacgat
    30781 cacgccagtg gtggcagtta ttcaggatgg tgtctcagtg gcatgttgat gtggttatcg
    30841 ttgagcggcg ttcgttcagt attgttgcag cagtagagct ggatgatgcc agccatttac
    30901 gaccggaacg cagacgccgg gatattcttc tggaagaggt tctgaggcag gctggtattc
    30961 cgttgctcag aagccacgat gccagaaaac tgctgcagat gaccggagaa tggctgaata
    31021 caacaggggc tgatcagcag tcaccggaac atcgtagctg acgccttcgc gttgctcagt
    31081 tgtccaaccc cggaaacggg aaaaagcaag ttttccccgc tcccggcgtt tctataactg
    31141 aaaaccatac tatttcacag tttaaatcac attaaacgac agtaatcccc gttgatttgt
    31201 gcgccaacac agatcttcgt cacaattctc aagtcgctga tttcaaaaaa ctgtagtatc
    31261 ctctgcgaaa cgatccctgt ttaagtattg aggaggcgag atgtcgcaga cagaaaatgc
    31321 agtgacttcc tcatcaggta caaagcgagc atacagaaaa ggtaatcctc tgacgcttgc
    31381 agaacgtcag caggcatccc tggcaagaaa acgagccaca cataaagaac tcagagtgtt
    31441 cattcctgct gcgttaaaag cgcaattgca ggtgatgtgt gaagctgagg gcgttaccca
    31501 ggctgagatg attgccgaac tgataaagca gaagagtgct tttagctaac taaaatgtag
    31561 cgacatcaca ttcttgcctg tttctggtgt gcgtgataga ttgctgatcg tttaaggaat
    31621 tttgtggctg gccacgccgt aaggtggcag ggaactggtt ctgatgtgga tttacaggaa
    31681 ccagaaaacc gaaaaccccg ataatctttc tatgtttggc gactcagaag attaccgggg
    31741 ctaacaagaa actgcataga agctgttgct ctatgcgggg agtatagtta tatgcccgga
    31801 aaagttcaag acttctttct gtgctcactc cttctgtgca acataagcgc aggatggtgt
    31861 ggctgatctt caacaaacgt attaccgcca ggtaaagaac ccgaatccgg tgttcacacc
    31921 ccgtgaaggt gccggaacgc tgaagttctg cgaaaaactg atggaaaagg cggtgggctt
    31981 cacctcccgt tttgattttg ccattcatgt ggcgcatgcc cgttcgcgtg gtctgcgccg
    32041 gcgcatgcca ccggtgctgc gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt
    32101 ccactatgac ccgctggcca accgcgtcca gtgttccatt accacgctgg ccattgagtg
    32161 cggactggcg acagagtccg gtgccggaaa actctccatc acccgcgcca cccgtgccct
    32221 gaagttcctg gcagagctgg ggctgatgac ctatcagacg gaatacgacc cgacaattgg
    32281 ctgcaatatc ccgaccgaca tcacgttcac gccggcactg tttgcctctc tcgatatctc
    32341 agaagaggca gtggcttccg cacgccgcag ccgcgtggaa tgggaaaaca ggctgcgcaa
    32401 aaagcagggg ctggatgcgc tgggtatgga tgaactgata gcgagagcct ggcgttttgt
    32461 gcgtgagcga ttccgcagct atcaggcaga gctgaagtcc cgcggcatga aacgtgcccg
    32521 cgcgcgccgc gatgcaggcc ggacccgcca ggacattgtc accctggtga aacggcagct
    32581 gacgcgtgaa attgcggaag ggcgttttcg gggaagcctg gaagccgtga agcgcgaaat
    32641 cgaccgtcgc gtgaaggaac gcatgataat gtcgcggaac aacaactaca cacggctggc
    32701 cacagcctca ccctgagagt gacctttact gaataatccg gcctgcgccg gaggcatccg
    32761 cacgcctgaa gcccgccagt gcacaaaaaa acagcaccac acacaaaaac agcctcatca
    32821 tatgccttcc ggtcagtcat gctccccctg tttttcacgc aaaacacgtc tcacagacag
    32881 ggaattccgc ttgtccacat tttactgcaa gggacttctc cataaggtta caaccattca
    32941 tgtcataaag cgccagccgc aggcgttaca gggtgcaatg tttcttttaa actccagtac
    33001 tgtatttcct ttaaactact taattacttc catttaaaaa gaaaacctgt tcactgcctg
    33061 tcctgtggac agacagatat acacctccca ccgcaagcgg cgggccccga gcggagtcaa
    33121 tttagttaca acatgcaaat atagcccccg gaaaaaaacc agttccgcgc agaactgaaa
    33181 ccacaaagcc cctcctcata actgaaaagc ggccctgccc cggcccaaag ggccggaaca
    33241 gagtcgcttt taattatgaa tgttgtaact acattgtcat cgctgtcagt gttctggctg
    33301 gaagtgttaa gtacacgctc gtaagcggcc ctgacggccc gctaacgcgg agatacgccc
    33361 cgactgcggg taaacccttg tcgggaccac tccgaccgcg cacagaggct ctgtcatggc
    33421 tgaaagcggg tttggctttg cagggccagg ggtgggtaag gtgaaatcta tcagtacgta
    33481 ccggcttacg ccgggctccg gcggttttac tccggtatca tatgaaacaa ctgagtgccg
    33541 ccttccatgc cgctggcgcg gcatcagtaa cctgtgactt tgtgtatatc atttgctata
    33601 tgtcgtcact gtgctattgt tgtaaagcaa atgatatacg gaggctttat gaaatcaggt
    33661 gttcagctca atctcagggc aagagaatct cagcggatac tcattgatac cgctgcagaa
    33721 attcttcata agtcgcgtac agacttcatt ctggaaatgg cctgtaaagc tgctgaagat
    33781 attattcttg accggcgggt gtttaatttt aatgacaggc aatatgaaga gttcatcgaa
    33841 atgcttgatg ctccggttgc tgatgagact gccatcgaaa aactgctggc gaggaaacct
    33901 caatgggagg tgtaacagca ccagaacctt tgtcctcatt tcatcaggcc gctgaatttg
    33961 tcagtggtga agctgtgctt gatgactggt taaagcagaa ggggctgaaa aatcaggctc
    34021 tcggagccac acggactttt gttgtatgca gaaaaggttc gcaacaaata gtcggttttt
    34081 actctctggc caccgggagt gttaatcata cagaggctgc aggcaacctt cggcgcaata
    34141 tgccagaccc tatacctgtt attatacttg cacgcctggc cgttgatgtg tcattccgtg
    34201 gaaaagggct tggcgcagat ttacttcatg atgctgtttg tcgttgttac agggttgccg
    34261 aaaatatcgg tgtgcgtgca attatggttc atgcgcttac ggaaaacgca aaacagtttt
    34321 atattcatca tggttttaag ccatcaaaaa gccaggagca aacattgttt cttaaactcc
    34381 ccggcaagta acaataattc aaactgtact ttttcttttc ctgtatccgg tttcctgtca
    34441 gtcacttctt ttttgtgcgc atgaaaaaaa gcctgcccac cgggcaggca taacagacaa
    34501 tcgcacagtc agtgttgaaa ttaatgatgt cacgcattat acccggtgga tacacaaacc
    34561 atccctgcaa atgcactggc atcctaatca acgtctgaaa caactgcaat ggccggacct
    34621 gtgatttcag agaaagttat tatgtttatt gaatataacc gaagtgataa ataaaacatt
    34681 atcatttcag taatatttat gctggttgcg tggttttatc tggtgatata aaactcagat
    34741 gtcctcattg tttgctgaaa aaacatcata atgatttttc tgctgtcctg tttccatatt
    34801 gccacatttt tattctcaga gcagatacca tctcccggaa taccggaaag ccggcgttat
    34861 cacgcgggat aagctcgtta ttcaccgcct tatctgaaag ggcatgcagg gacagggcgg
    34921 tttcctctct ggtgacgtcc ggtcattcag gtaatcaaca acaacgaaaa tgggcgataa
    34981 agataaaaaa ctggcctgcg gcaaagcagg ggggatatcg gccctggcgc tcgggctggt
    35041 aaaggtatca ccattcacgg taatgtgaat atcggtgaga atgtgattat caggcagaac
    35101 acaaccacgg gcgaaaagcc ctctgacagc agagaaaatt atattgttac tggtgataat
    35161 attgataccg gtgcccgtgc gtgtattccg gatttaatgt aaaacaggcg gtaatgtaaa
    35221 aaccggagcc ctgtctttta ttatggaaga tgttccggat aactgtactt atgtcaccag
    35281 gacagaatca cgggtaatca gacgtgatgt atgatgatat tattcgctgt ataatgatat
    35341 tgttgaccgg aatttaatct tgtcgggaga tttatggcca gaaaccagga aaagaaaacg
    35401 cccgtgattg tggtgaaaaa gcggcgcgtt atttcaccaa cacctgtcgc cgataaaaca
    35461 gagacggtta ttccgtctgt accggaagag cctgttcctg ttgctgatgc ggggaggaat
    35521 ctgaccacac ccatcccgga tggggctcag gcgccggctg cctccggaaa gaaaaagaaa
    35581 ggaagacctc cacgcaggcc cctcagagca cactggaccc gtgagttcac tgaccagtgt
    35641 atgaaaaagg tcaaagtgct tttcccgcat cttcgcgctg aagatggtgg ttttctgccg
    35701 cttaaggtcg gtatctcccg ggatattcct gcctggctgg ctgagcaccc tgaagccgga
    35761 ctcacccggg atgaatggga ctgtgccgca tcctgtatca cctcccgccg tgtctatctg
    35821 ctgcgtacct ctgtcaccgg tgtcacccgc tatgacctcg acggtaagcc tgccggacag
    35881 gtttctgagg atgaggcaca aaatgcccgg cgctggctgg ccatccggga gcgccgctgg
    35941 caggaaagac aggccacact gacaacggac ggtagcgctg aaccggtggc ggaatgattt
    36001 ctcacccgga catggtgtcc tgttatatac cgacagcgtt gtaccgtgca catgaccgtt
    36061 tacttcgtat gtccgggcgc tcctgagact ggttttacag tctcagggat gactgcctgg
    36121 cgctcacgga tgtctctttc tctgtcttcg ctgcctgagt ggcccggtgg caggatattg
    36181 cctgcctgtg gcgaaagcag catgataccg gtaccgtgtt ttacaggagt aacggtatgt
    36241 ggtttttttc aacagtgctg tgtctgcttg tcattatctg ctgtgtggcc ggtattgtga
    36301 atgcaatggc agtacggcgc gagttcaaaa ggagcaggga gcaggagtac catgcgcgga
    36361 tacgggaggc cacgggtgaa cgtattctgc gggagcggta tgaacacttc cggcggcgaa
    36421 gggaacgttg aataaaaaac ccccttcagg aaagggggaa tcacttacgt tttgatcaag
    36481 ggtaatgttt ttatagtttc tgctctacgt gcttcttgcg taccacacag ggtacaacat
    36541 taatttaata taatgataac gttcatgaaa tccgatgagt gactgttgca cgaccatcac
    36601 ctgtttgtaa cctgcaggtt taaacaaacc ggccttcgcg cgcagttccg gtgccggagc
    36661 ctcctgaaca ttttcaccgt acaaggacat tcaggaggca cgcacgaaag ggaatacagg
    36721 ctgatattca ggaatggcgt ctgtcccatc actgatggta tgacaaatca cggccgttgc
    36781 tgcgcatctg ccgtgcggaa aacatccctg actgaaagat gctgagattc gccggggtaa
    36841 tggtccgcat ccccgccatc attgcgctga cctttatgga aatatggccg ctgaagcaac
    36901 gcataatcat cttaagctta cttcaacggg cctttatccg tattcacact gaccgttaaa
    36961 gatgcttttt aaaccattgc gtctgtactg caacgacacg ttcaaaaaac tcaccctcat
    37021 aaagatcata atgccgggct gccgcttcct cgtataatgt tttatcagca gaagcaacag
    37081 catcatataa tgctttcccc tgctccggag gattaacgct atccctgccc gcaataacaa
    37141 cgagcacagg acactgtact ctggctgcac tctccgccgg tttatattgc aatgtttcca
    37201 ttacggtaag gaaggggatt tttatatcca ttccgggatg ccggattttg actttcccga
    37261 agaactcctt cgattcatta tcactcagta cccgggtaac ccccacaaac atctctttac
    37321 cggtgttttt cttcttctct gccattttat tcagtgtgga cagaaatgat gttctctccg
    37381 gctcactcat ccctccggta accagagtat caccgtcagc aaaggccagc tggctgacca
    37441 gacatttcac ccgttgatcc tgcgctgctg cactgaaaac atgacagcca cccagcgatg
    37501 tgccccacag tccgatacgc tgattatcaa tatctgtctg ttgttctgcc cagctgataa
    37561 ctgaaataat atcttcggtc tgcattgccg gaaccaggcg gccccgctca ccttcactct
    37621 caccgaatcc acgataatca aaagtaatcg ttgcgaatcc ggcctctgta aacgcattcg
    37681 caaaggaggg gagcagaacg ttccggatac cgcaaaatcc gtgacataag ataatcaaag
    37741 gcggtttttg atttccttcg ggcatccgga gtgtcagggc aataccttcc gggagtttgt
    37801 gatccgtaat tttcataaat atcctgtgtt acgtcgttta ttaataatca gaatcagcgt
    37861 catacctgtc tcagcgccgg gcgcccgcta acgccagtgg gcaggagggg aatggcagaa
    37921 gaagtccccg gcagaagcgg agtcatgata tcagtacggt tgatattccc ccggacgatg
    37981 tacatgagac ggtgacttat accggaacag tacgaacaaa tccggcgaca aagacagcac
    38041 gcgcaaaaat acctttaagc ggagtactta catttgcgct gtgaataatt tttaatacgt
    38101 cctgcgttgt ctcaggaaat taaatatcaa tttaacatgg ctgtaacctg catgaaatcc
    38161 gatgagtggc gcgacaacag tgatattttt cgtcagacgg tgttacggtg aatatgctgt
    38221 ctgctcagcg ctgttaatcg ggatggtatc ttttccggcc ggggtgtttt tatgacaggt
    38281 cgggaataaa tatatgtcgt tttgttatat gaatatattt aaattccctg agtgtttatc
    38341 tctgtcttct gtatttcagt gcgtgcagag tcatttttta ctgacgtaga tgaacgcatt
    38401 ctcctgtaag attatggtgc ggggatctta aagatatcgc ggggtgactc tgctttataa
    38461 cggggcagac gttgtgcagg cttttttgag cgctgaaccc gtatcagcgt gtggatatta
    38521 tacacatctg tgttaccgga ccgctgcctg tatatccgta ctggttcatc aataggaatt
    38581 taaaccccca aaagattaaa aaccccccca aaaaaggacg ttaattcaac accaccagtg
    38641 tgccttaaca tcgatgactg ccagtcagat gtttcccgct gactgttttt tgtgatgact
    38701 gccgattttt cgccctgttc ccctaaaaat ggacgcaact tcccctccgc ggctctcagc
    38761 cgcaacactg cattcgggcc agtggctcat gcatcataac cagctctgcc agacggtatc
    38821 cccgcttcag ccccgtaaac cgcatctgac ttccgcacag gatgcatttc agcgggtcaa
    38881 ccttcagtaa ccgctgatac atccttctcc acctgatttg catcgccgtt tttctcaccg
    38941 tctccgttat gacgtacacc acctcttcca gtaaccgccg tttcgccgga ctcaaaaaac
    39001 cgtagtacct caccatacgg aaccccttat ccgccacatg ccacgagaac ctttccatga
    39061 actcatctcc actcatctcc actcatcacc aggtattatt cccgttttgt gcggtggctg
    39121 ttgtaacgca gaccaatttc atcctgaccg gcatagtgtt ccagacggct cattggcacc
    39181 ggcggctttt tcaggtaaga gccaaagtac accgccacat gcgtggcgtt atccatcacc
    39241 cgggatacgt tgacattcca gccacgccgg tagtgggtgt ccaggaagcg attccattcc
    39301 cgtctgccgc ttccctctgc tgccagctca gccggtatca ccagctccgg gtatttccgg
    39361 gacagtaatc gcgttatccg gtaacgccac atgcgcatca ccttacgggc gtaaaaatga
    39421 aggtttttcc aggtgtgagc tgacgtcaca ccgccggctg ttgtcgacag gtgtatgtgc
    39481 ggatgccact gctggtctcg tccccatgtg tggattaccg tgaatatccc cggctccacg
    39541 tccgcctgac ggcagatttc cagtatcaca ttcgctgcga tacggctcat ctctgccagc
    39601 aaccaccggt tgtggaatat cagggaccag tactggcagg gaagtgtgaa cacaatatgc
    39661 tgccacgggc agtcgggagc cagactcagc agatactgta tccactgtgc gccagccttc
    39721 accccgcagt gcgggcagga gcgactttta caccggaagc agaccttttt tgtgtggcaa
    39781 cagtcctgtg aggaacaaca ccactgtgtg taccccatca gcgtggtccc gcacgccatg
    39841 atttttgtca ccgactcaat caccaccgga cgcacagacc cttccggctg cttctccagc
    39901 cagttaagcc agcggtttcc ctgctgaaaa atatcggcaa aacggggatg catcagaagg
    39961 gcgggtgact ccgtccggcc agtgaaccgt gccgcattcc ggacagtaca taccgccggc
    40021 gctgataccg gcaagcatgg tcgcaaactc ccgctccgtg cagcgggcta tttccggata
    40081 cccttcgcca tcaacacgta caaaccagaa gaccagcttt ttgtttccca catccacaaa
    40141 gaacggaata ttcaggtctg cgcagcattc aacggcatcg tcaaaactat caaagcgcag
    40201 aacttctgcg tcttcttcgt caaagaagtc atcgtcgtga agcgtcacga catagcggga
    40261 gagtttgctt ctttgagggg cgggttttcg ttttcggggt ttagctgaac gggccatata
    40321 actacctgaa agacaatgat attgtttgtt tttataacgg taatctcagg ccatgacaag
    40381 ccgcagccgt caggctgcct actcgatatc cagatctttt tttacggaaa ttccgtctgc
    40441 attcaaatgt gctttagaaa tctgaccgaa attaattgtc tgatctgctg atttttgtgc
    40501 aataccacaa ggagcatcaa ttactgtacc tttgaagtta ataatgccct atccctgatt
    40561 agcagcatta acgccaaatg aaacagtaga agcaagcatg gttgcaataa taactttatt
    40621 catataacaa aaccctgagt tgaaaaaata cat
//
LOCUS       DAZFJL010000023        39903 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFJL010000023 DAZFJL010000000
VERSION     DAZFJL010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.023"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(40..1899)
                     /gene="gss"
                     /locus_tag="ACHJ8C_001249"
     CDS             complement(40..1899)
                     /gene="gss"
                     /locus_tag="ACHJ8C_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO5551139.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2104..2970
                     /gene="yghU"
                     /locus_tag="ACHJ8C_001250"
     CDS             2104..2970
                     /gene="yghU"
                     /locus_tag="ACHJ8C_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO5551140.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRINGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3093..3341)
                     /gene="hybG"
                     /locus_tag="ACHJ8C_001251"
     CDS             complement(3093..3341)
                     /gene="hybG"
                     /locus_tag="ACHJ8C_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO5551141.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3354..3695)
                     /gene="hypA"
                     /locus_tag="ACHJ8C_001252"
     CDS             complement(3354..3695)
                     /gene="hypA"
                     /locus_tag="ACHJ8C_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO5551142.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3688..4176)
                     /gene="hybE"
                     /locus_tag="ACHJ8C_001253"
     CDS             complement(3688..4176)
                     /gene="hybE"
                     /locus_tag="ACHJ8C_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO5551143.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMLLSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4169..4663)
                     /gene="hybD"
                     /locus_tag="ACHJ8C_001254"
     CDS             complement(4169..4663)
                     /gene="hybD"
                     /locus_tag="ACHJ8C_001254"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO5551144.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4663..6366)
                     /gene="hybC"
                     /locus_tag="ACHJ8C_001255"
     CDS             complement(4663..6366)
                     /gene="hybC"
                     /locus_tag="ACHJ8C_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO5551145.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPAKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6363..7541)
                     /gene="hybB"
                     /locus_tag="ACHJ8C_001256"
     CDS             complement(6363..7541)
                     /gene="hybB"
                     /locus_tag="ACHJ8C_001256"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO5551146.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7531..8517)
                     /gene="hybA"
                     /locus_tag="ACHJ8C_001257"
     CDS             complement(7531..8517)
                     /gene="hybA"
                     /locus_tag="ACHJ8C_001257"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO5551147.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGSGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8520..9638)
                     /gene="hybO"
                     /locus_tag="ACHJ8C_001258"
     CDS             complement(8520..9638)
                     /gene="hybO"
                     /locus_tag="ACHJ8C_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO5551148.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9827..10114)
                     /gene="yghW"
                     /locus_tag="ACHJ8C_001259"
     CDS             complement(9827..10114)
                     /gene="yghW"
                     /locus_tag="ACHJ8C_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO5551149.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKYCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10233..11120)
                     /gene="yghX"
                     /locus_tag="ACHJ8C_001260"
     CDS             complement(10233..11120)
                     /gene="yghX"
                     /locus_tag="ACHJ8C_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO5551150.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVTRRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQSYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11277..12317
                     /gene="gpr"
                     /locus_tag="ACHJ8C_001261"
     CDS             11277..12317
                     /gene="gpr"
                     /locus_tag="ACHJ8C_001261"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO5551151.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNAIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLHEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12357..12851)
                     /gene="yqhA"
                     /locus_tag="ACHJ8C_001262"
     CDS             complement(12357..12851)
                     /gene="yqhA"
                     /locus_tag="ACHJ8C_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO5551152.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13042..13926
                     /gene="yghA"
                     /locus_tag="ACHJ8C_001263"
     CDS             13042..13926
                     /gene="yghA"
                     /locus_tag="ACHJ8C_001263"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO5551153.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAITDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     ASYVTAEVHGVCGGEHLG"
     gene            complement(13965..14390)
                     /gene="exbD"
                     /locus_tag="ACHJ8C_001264"
     CDS             complement(13965..14390)
                     /gene="exbD"
                     /locus_tag="ACHJ8C_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO5551154.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTV
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14397..15131)
                     /gene="exbB"
                     /locus_tag="ACHJ8C_001265"
     CDS             complement(14397..15131)
                     /gene="exbB"
                     /locus_tag="ACHJ8C_001265"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO5551155.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15383..16570
                     /gene="metC"
                     /locus_tag="ACHJ8C_001266"
     CDS             15383..16570
                     /gene="metC"
                     /locus_tag="ACHJ8C_001266"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO5551156.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLLPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16710..17369
                     /gene="yghB"
                     /locus_tag="ACHJ8C_001267"
     CDS             16710..17369
                     /gene="yghB"
                     /locus_tag="ACHJ8C_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO5551157.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17409..18308)
                     /gene="yqhC"
                     /locus_tag="ACHJ8C_001268"
     CDS             complement(17409..18308)
                     /gene="yqhC"
                     /locus_tag="ACHJ8C_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO5551158.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18502..19665
                     /gene="yqhD"
                     /locus_tag="ACHJ8C_001269"
     CDS             18502..19665
                     /gene="yqhD"
                     /locus_tag="ACHJ8C_001269"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO5551159.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPSRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19770..20597
                     /gene="dkgA"
                     /locus_tag="ACHJ8C_001270"
     CDS             19770..20597
                     /gene="dkgA"
                     /locus_tag="ACHJ8C_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO5551160.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20797..21723
                     /gene="yqhG"
                     /locus_tag="ACHJ8C_001271"
     CDS             20797..21723
                     /gene="yqhG"
                     /locus_tag="ACHJ8C_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO5551161.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVHFRIFKDDKEKTSQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     AIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTTDSARIHVRFTLTNGNNEEQELILQR
                     REGKWEIADFIHPNSGSLLKQIEAKTAARLKQ"
     gene            21774..22031
                     /gene="yqhH"
                     /locus_tag="ACHJ8C_001272"
     CDS             21774..22031
                     /gene="yqhH"
                     /locus_tag="ACHJ8C_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO5551162.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22073..24292)
                     /gene="ygiQ"
                     /locus_tag="ACHJ8C_001273"
     CDS             complement(22073..24292)
                     /gene="ygiQ"
                     /locus_tag="ACHJ8C_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO5551163.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPNNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTLPALTKHTPMATQRQTPATAKKASSTQSRLQNAGAKKRPKAAVGR"
     gene            complement(24403..25815)
                     /gene="ftsP"
                     /locus_tag="ACHJ8C_001274"
     CDS             complement(24403..25815)
                     /gene="ftsP"
                     /locus_tag="ACHJ8C_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO5551164.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(25890..26627)
                     /gene="plsC"
                     /locus_tag="ACHJ8C_001275"
                     /gene_synonym="parF"
     CDS             complement(25890..26627)
                     /gene="plsC"
                     /locus_tag="ACHJ8C_001275"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO5551165.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(26862..29120)
                     /gene="parC"
                     /locus_tag="ACHJ8C_001276"
     CDS             complement(26862..29120)
                     /gene="parC"
                     /locus_tag="ACHJ8C_001276"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO5551166.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDERRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFSDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVAGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29258..30865)
                     /gene="ygiS"
                     /locus_tag="ACHJ8C_001277"
     CDS             complement(29258..30865)
                     /gene="ygiS"
                     /locus_tag="ACHJ8C_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5551167.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDKWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKVLLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSKWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSWQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQAAQTADATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(30974..31456)
                     /gene="ygiV"
                     /locus_tag="ACHJ8C_001278"
     CDS             complement(30974..31456)
                     /gene="ygiV"
                     /locus_tag="ACHJ8C_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO5551168.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTEQRLETDVYVPLA
                     "
     gene            complement(31509..31901)
                     /gene="ygiW"
                     /locus_tag="ACHJ8C_001279"
     CDS             complement(31509..31901)
                     /gene="ygiW"
                     /locus_tag="ACHJ8C_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO5551169.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            32053..32712
                     /gene="qseB"
                     /locus_tag="ACHJ8C_001280"
     CDS             32053..32712
                     /gene="qseB"
                     /locus_tag="ACHJ8C_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO5551170.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            32709..34058
                     /gene="qseC"
                     /locus_tag="ACHJ8C_001281"
     CDS             32709..34058
                     /gene="qseC"
                     /locus_tag="ACHJ8C_001281"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO5551171.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNANGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVDFGNAEQGGFEAKVSW"
     gene            34168..34749
                     /gene="mdaB"
                     /locus_tag="ACHJ8C_001282"
     CDS             34168..34749
                     /gene="mdaB"
                     /locus_tag="ACHJ8C_001282"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO5551172.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            34780..35094
                     /gene="ygiN"
                     /locus_tag="ACHJ8C_001283"
     CDS             34780..35094
                     /gene="ygiN"
                     /locus_tag="ACHJ8C_001283"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO5551173.1"
                     /translation="MITVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GL"
     gene            complement(35139..36026)
                     /locus_tag="ACHJ8C_001284"
     CDS             complement(35139..36026)
                     /locus_tag="ACHJ8C_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO5551174.1"
                     /translation="MIQKDKVRVDIFGERFRTRASQLTPGLRVVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(36023..36970)
                     /locus_tag="ACHJ8C_001285"
     CDS             complement(36023..36970)
                     /locus_tag="ACHJ8C_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO5551175.1"
                     /translation="MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(36981..38018)
                     /locus_tag="ACHJ8C_001286"
     CDS             complement(36981..38018)
                     /locus_tag="ACHJ8C_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO5551176.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLLLLGGLFLFAVGILIFGLMHGS
                     FSVPASEVGRALFSPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(38027..39010)
                     /locus_tag="ACHJ8C_001287"
     CDS             complement(38027..39010)
                     /locus_tag="ACHJ8C_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO5551177.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGFVLALYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPYELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(39007..39816)
                     /locus_tag="ACHJ8C_001288"
     CDS             complement(39007..39816)
                     /locus_tag="ACHJ8C_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5551178.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
BASE COUNT         9973 a        10735 c         9981 g         9214 t
ORIGIN      
        1 ttattcatca taatcagtac ggagttgcta caacatgtat tactctttca ccacaattaa
       61 cggctcaata tcactctctt ttttgatgac cagtgattca tcaccgcgta agcaagtccc
      121 accgtagttg ccgccaacgg tgaaggtaca tacctgaatg tatttaccgt ccactttcgg
      181 caaacaccac agttgctgat agatgttttt ctgctcggca aatttaccgc tggttttgtc
      241 cagcacctct tcatgatggc tgacgaggtc gatgttactg ccacagcgac cggcgatcgg
      301 tttaacggca taaccggttt tcaccagctc atcattcacg gtgaagtcag tatccaacag
      361 ataacgatgg tgcgggaaca gagaccagag gatcggcaga atcgctttgt taccggggat
      421 caccgtccat agcggctcaa aaaccagcac ttccgggcgc agcaatacgt cgataagacg
      481 cacttcattt tgcggatgac cggtacggat cggcaccgca gcaaactcac ggtcgctaac
      541 ttcacgaatc tgatcaaacg cggtttccca cgcccaggtt ttccacacgc agttaaccag
      601 tcgcccttcc ccatcaatca gttgcccggc agcatcccag cccagttcat ccagcccgcg
      661 caagatacgc gtttcaaagc ccgcctggtg cagcgcctgc tccataaact gcgcgtgata
      721 gttttcctcg atatctttgt cctgcatgat atggacaaac ggacgtgcac gactgtgttt
      781 ccaggcacca gccagttcgt taatcagccc ttccgccgga ttgaagccgt tgcctttata
      841 gccctgctcc gcccaacgtt cgaggatcaa gccagcttcg gtatgacagg aggcggagtc
      901 ggcgttgtat tcgtagacct tcaggccacg ttcgtccata cagaaatcca tacgaccggt
      961 gatcatatgg tgacggcgac gctgccagga gagacgcaaa cgtggccaga ggatttttgg
     1021 gatgtcgaac agcgccagca ggttgtcatc tttcagcacc ttgtcggttg cgtgaagata
     1081 catcaggtgc agttcgttgg tggctttaat cagctcctgc tctgcgctct cggtaatggt
     1141 gtagtaatga tacggatcct gattgatcac ctgaccgttg gcctgcacat aggcgttttg
     1201 tagcggatct ttttcatcca gccatttacc gtcaaactgg cctttgtttt ccaggcgcgc
     1261 tccgctgatt ttcaacagct cgcctgcaat ttccggctgc ggtaagctgt attcagtatc
     1321 ttccgtctgg atcatccagc cgagaatggt ggtgtcatca aaagtgtctt taagggtata
     1381 gcagccgttt tcgaccacca tctccagctc gcgcgtccac tgttgccctt gcggtaacgg
     1441 ggaatgaatc acgttctgtt ccgcaatacg gactttgttg ccatgcaatt gggtaatgat
     1501 ggcgacatga ccagtgtctt taaattcacc gcctttatcc cagataagaa gcgcacccgc
     1561 gaccggcgca cgcggcgagc cgttaggaaa tgcctgcaat ggcaggatgt tgtcattaac
     1621 cacttcacgc aggaagcgca gcgagaaaat ctcccacgcc atacccacgt cagtgaagac
     1681 cacaccgtaa ttcagaaaga gaaaacggcg agcaaattca acgcattgcc atttgtggcc
     1741 catatattcg tcgtcgatat agctacggaa tacggcgtca tcttcgtatt cctgcggatc
     1801 gagagaactg taatctgaag agtagattgc taccccacct ggggcgtagc ccaataatgt
     1861 cccgaacggg gcatcctggc tggtcgttcc tttgctcatc actttacctt taacaataca
     1921 acctaagcag ttggcgtaat tgttgtgctc tgattacctg ctcagcgaca ttaaccggac
     1981 agaggtcaga ctaatcatac acctcatcgc agcggctggc gcaaagggtt aaaaaattca
     2041 cattctgtac agcaagtgac ctgctacact gcttcaacac taccactcag aaggcaactc
     2101 actatgacag acaacactta tcagcccgcg aaagtctgga cgtgggacaa atccgccggc
     2161 ggcgcgttcg ccaatatcaa tcgcccggtt tctggtccga cgcatgaaaa aacgctgccc
     2221 gttggcaaac acccattgca actttattcg ctgggaacgc cgaacggtca gaaagtaacg
     2281 attatgcttg aggagctgct ggcgctgggc gttactggtg cagagtacga cgcctggctg
     2341 attcgtattg gcgatggcga tcaattctcc agcggctttg tcgaagtgaa cccaaactcg
     2401 aagatcccgg cgctgcgcga tcatacgcat aatccgccga tccgcgtgtt tgagtctgga
     2461 tcgattctgc tttatctggc ggagaaattt ggctacttcc tgccgcagga tctggcaaag
     2521 cgtactgaaa cgatgaactg gctgttctgg ctacaggggg ctgcaccgtt cctcggcggt
     2581 ggttttggtc acttttacca ttacgcgccg gtgaaaattg agtacgccat caatcgcttt
     2641 accatggaag ccaagcgcct gctcgacgtg ctggataagc aactggcgca gcataagttt
     2701 gttgcaggcg atgagtacac cattgcggat atggcgattt ggccgtggtt tggcaacgtg
     2761 gtgttaggcg gcgtgtatga tgccgcagag tttctggatg cgggaagtta taaacacgta
     2821 caacgctggg cgaaagaagt gggcgaacgt ccggcagtga agcgtgggcg tattgttaac
     2881 cgcatcaacg gaccgctgaa cgagcagttg catgaacgcc atgacgccag tgatttcgag
     2941 acgaatacgg aagataagcg tcaggggtaa gggttggtat tcgtcgcagc aagccatcca
     3001 ggccggataa ggcgttcacg ccgcatccgg caatcgagca caatgcctga tgcgacgctg
     3061 ccgcgtctta tcaggcctac aaaagctaat catcacgcgc tggtaatgtc gtaatccatt
     3121 tggcgcagcg cgtctaatgt ggctttggct tcatcttcgt cgatgatgct catggcaaat
     3181 ccgacgtgta ccagtaccca ctggcccagc agatcggcag ggttaccttc acaaatcagg
     3241 gcgatattca cgtcgcgctt gataccacat acttcaacct gcgcaagctg gtgaatatct
     3301 tcaccgacag ccagcacctg gcctggaacg ccaatacaca ttgttaactc cggttattca
     3361 acttcaatac ttttgacgat tagcgaatcg ccggtatcga cacgcaaccg ctcgccatga
     3421 cagatcggac actgcgcatc gtgctgatga atctccacca cctggctgca atcccagcac
     3481 caggcctggg cgggtttata gacgatatgt aaatcgcacc cttgcgccac cgttccgtgg
     3541 cagacaattt caaaactaaa acggacggcg ctctcctcaa cgcaggagag cgcaccaatt
     3601 tccagccaca cggcggtgac gcgcttaaca tcgtgctgct ccgcctgccg ttggataatt
     3661 tcaacggcgc tctggcaaag cgacaactca tgcattttcg ccactcctgc ggccaaatag
     3721 cagggcacga cgccctgcgt gtggtacatc cggattggtg actggcagcg aaaggagcat
     3781 tcgggcgcag tcatccgtca gccgttggcc ctcttcaatc gacatgctgt gcgaaagcgg
     3841 cgacatcagc gagcaggaga ggtattgcga aacaccatcc agttcaccaa cagtaaaggt
     3901 catagtgcca tacggcagtt gcagaccaat tttttcactc actttgcgca gcggccagag
     3961 ttgatccggg ccggggaaga taactgcact cagcatccac ggggtgatca cacaccccgt
     4021 ccactgacct tcgaacagcg taaagtcaga aacatacacc ggcattgaag gatgcagaaa
     4081 agagagatca tgcatcgaac gccgggcaat ttcttcaaaa gctgcctgta cttgcgcctt
     4141 cggggaggtc tggaaacctg ctatctcttc agtcatgaat cgcctcccgt gggatggctt
     4201 ccacgcctga ttcacgcagc gcagccagaa cctgctcaag cgcaggttca atcatcgctt
     4261 cgaccgtcgg cgtcaagcca atgtgcggct ccagtgattc cgggatcacg ccgaccaggg
     4321 tcagcttttt cggaaactca ccggtgaagc gcagggccga caatacgtcg gccaggccaa
     4381 gctgatgcgg agagattttg ttggtaaaca acgccggaac ttcttcatct cgcaggatca
     4441 tcatcgttcc cggcgcgttc tttttcgaga caatggcatc cgcaataatc agatgatcgc
     4501 gatttgccat gtcgccaagc agctccattc ccgccgttcc gccatcgagg atctcaacat
     4561 aatccggcag aatgtatcgt tgctctaacg cttcaacaat tcgcacaccg atggcttcat
     4621 cggtcagcaa aatattgcca acccctaaga ctaaaatacg cattacagaa ccttcactga
     4681 aaccacttcg ttgccgtcgg catccactac gtgtaccgca caggccatgc acgggtcgaa
     4741 ggagtgaatg gtacgcacca cttccagcgg tttattcgga tcggcaaccg gtgtacccac
     4801 cagcgactgc tcgtaaggac cgacgtcatc attgaagtta cgcgggccag agttccaggt
     4861 tgatggaaca actgcctggt agttactgat aataccgtct ttgatcacca tccagtgaga
     4921 gagcataccg cgcggtgctt caaggaaacc aacgcctttg aactcacctg tagccggaat
     4981 gttcggcttc acaaaggtgg tgtgatcgcc tttgccgata ttggtgatca gtgcactgta
     5041 ttgattttgc aggatatcct gcaattcgca gcagtgaacg gtacgaccaa taatacggcc
     5101 cagcgtggag tgcagctgcg ccacttccag cgtgttgcca gtcagtttct gataaatcgc
     5161 aacgatttca ttcagtttat tctgggtaga ttcgcgacct gccgccagtt tcaccagcat
     5221 gttagccagc ggacccactt ctaccgtttt gccgtagaaa gtcggtgatt tcacccagga
     5281 gtatttaccg tcgtcagacc aaccgtcgta agccggaatg gtggtacctt cccacggtgc
     5341 ctgcggcgct tcgtctttat accaggagtg cttcgcgctt tcctgaatcc ctttgatcag
     5401 gtattcatcg gagtgggaag tgatcggacg atacgaggac agatccgcat tctcaatgta
     5461 gccgccaggg aacaggaagc tgccgttttt gctgtcagtc gggaattccg gcacgctcag
     5521 gtagttcacc gcacctttac cgcgcgtcag ccattccggg tagaacgcgg cgataaccgc
     5581 ggtatcaacc ttgtaaacct gttcaacaaa gtcactcagc ttgtcgatga aggacttgat
     5641 gtacatcaga cgctcaagat tcagcacgcc cagaccgtcg aggttgatcg ggttcgcgac
     5701 accacctacc gccaggttct gaatatgcgg cgttttaccg cccagcagcg ccacaacgcg
     5761 gttagcgtca cgctggcact ccagcgcctg caggtagtgc gctaccgcaa tcaggttcac
     5821 ttccggcggc agtttcattg ccgggtgacc ccagtagcca ttggcgaaaa tacccaactg
     5881 accgctggca accagatctt tgatcttgtt ctgaactttg gtgaactctt ccgggctgtt
     5941 cagatgccag gtcgaaacgc ctttcagcat ttcggaggct ttggctggat cagcttgcag
     6001 tgcagaagtg atgtccaccc agtccagcgc cgaaagctga tagaaatgaa caatatggtc
     6061 atgcgtggtg tgcgcagcca gaatgatgtt acggatgtat tgcgcgttaa ccggaacgtc
     6121 gatattcagc gcactttctg ccgcacgaac ggaagacagc gcgtgagtgg tagtacatac
     6181 gccacagata cgttgcacaa tcatccatgc atcgcgcgga tcgcggtttt tcacgatctc
     6241 ttccatgccg cgccacatgg taccggaagc ccatgctttc gaaacgacgc cattttcgat
     6301 ttcgcaatcg atgcgtaaat gcccttcaat acgggttacc ggatcaatag taattctctg
     6361 gctcatgctt tgctcgcctc atgacgatta tgatcgtttt gttttaaagg aggaagtatc
     6421 ggcagtagac gaatgagtac gatataagcg caaatctcaa tagccacaaa accaatagaa
     6481 atcaacagtt cttcccaggt cgggaagtag gcgtaaccgc cgcccgggtt gaatgccacc
     6541 agcgaatagg tcagacgcca ggttgcacaa cctaacagcg cgctcagtgc tgacaggaac
     6601 agcatgcggg aatcattacg cagcttcgcc acacgcagaa cgaccagcgg gaagagcatc
     6661 agcaggactt caatccagaa catcacggag tagaagtcac cggcaaacgc taacgacagc
     6721 ttgtcgcgat agataagctc gccaaagcgc agcacgatga aaatcgccag caacacactg
     6781 atggtgttgg tcagcttgac gaacaggctc ttttcatctg gaccgttgcc acgcagaccc
     6841 gcctgcacca gcgaaccttc aaagatgaca atcgagaagc ccatgatgaa cgccgtcagc
     6901 agcgagaaca gcggcaacat ttcatagctc tgccacaacg gatgcacctt gtagcccgcc
     6961 gagatcatca gcgaccccat tgaagactgg tgcatggtcg gcaacagcgc gccaagtgca
     7021 atgatgaaga acatcacctt gttcagacgc ttgagagaaa ccttccagcc cagacgttca
     7081 aacagtgccg gagcaaactc cagtgccatc acaccgatgt agatggtcat acagaccgcc
     7141 gtctcgaaca gtaccgagtt cacgttgaag tgacccggaa tgtagaagta cggcaggttc
     7201 cagtagcgac ccacgtcgat agtgatcgac aagccaccca gtgagtaacc aaacagactc
     7261 gccaacagcg ccggacgcac cagcggatgg tattgcccac ggttaaagac gtataccgcc
     7321 cacgccagcg cccagccgcc acaggcaaag ccggtgccaa tcagcaggtc aaacgcgatc
     7381 cacacgcccc acgggaagcc gccgttcagg tcagagacag agcccagacc gaacaccaga
     7441 cgcttcacaa tcaggagcat acagatgacg attaacggtc caaaaatcat gaccggttta
     7501 ctgatgattt tgccgcccag cggttgtgga tcatgactca tgatcgtctc ctccgtcgtg
     7561 atggtcgttt ttggtgttgc gacgaaccag cacggttaag cccgccagca cagccagtgg
     7621 tagcatcatg cctttataca gggtgtgttg aatattttcg gaacgcgcac cggtagaaag
     7681 atcgtccagt ttcggcaggt cgagattttc ataaggcaca cccgtcagta ccagaacctg
     7741 agtaccgccg ccctctttct cgccgtacag atgcggataa tatttcggca ccgtatgcag
     7801 gtaagtgtcg ccagatttca gcgtctgacg cggatagtgg tattcgctgc ctggcttcag
     7861 cgccagacgt tttttcgcct cagccatcag ctcttcacgc gtaccaaaaa tcaccgcgcc
     7921 cgccgggcac acttctacgc agccaggcag accgccttta tcaagacgtt ccacaccttt
     7981 ctggttgcac agttcacact tatgcagcgc accaaatggg ttgttgtagt cgtacttcgg
     8041 cacgttgtat gggcaggcga ccatgcagta acggcagcca gtgcacacat ctttgtcgta
     8101 atggacaatg ccggttttcg gatctttttt cagcgcagag accgggcaca cagagacaca
     8161 attcggatcg acgcagtgca tacactgttt cttaatgtac gcgtagccgt tctcctcctg
     8221 gtctttgttg acccccgagc cgctggtcca cacctgaatg atgttattgg tgtacggcga
     8281 cagtttgtcg ttgttcgacc aggtctgttc cccttgcggg ttacgttcag ggaaattgat
     8341 atcctgacac ttggtgacgc aagcctggca gccaacgcac aaggtcgagt cgtacaacat
     8401 ccccagcgat cccggaattg gcgggcggtt ttcagcagcc gcatgactga cagacggcag
     8461 cgcgcccgtc agcaatgccc cgcaggaggc tgctttaata aaattacgtc tgttcacggt
     8521 tattctcccc gtgagtcagc gttatctttc ttttgctgac gacccagttc acgcaccgcc
     8581 atcacgctga caccggcaac cagcccaacc acaccgccga gcaaaccaat agcgcccgca
     8641 gagacgttgc cgccttcttt agcgttaacg tccggtttct gtgaacgcgg agtctgattt
     8701 tcgacgttgg caagctgatg gatgccttta tggaagccga taccttcttc gttacagcca
     8761 tagcacgggt gaccaatcgc taccggccac acgccgccaa cgtcgcagaa ttgcagcgtt
     8821 gagcagttgc cgtatgtttc tggcccttta cagccgaggt ggtacaggca ccagccttcg
     8881 cggtggcctt catcaccgaa ctctttggca aaacgaccag catcgaagtg cgggcgacgt
     8941 tcgcagtgtt cgtgaatcag acggccatag gcgaaggtcg gacggttttt gtcatccagt
     9001 ttcggcggct tgccgtaagt gatgatgtgc gcaacggtcg cgaggaagtt gtgcgggttc
     9061 ggcgggcagc ccggaatatt gataacggtt ttgcctggca gaacttcttg caggctgact
     9121 gcgccagttg ggttaactcc agcggcggca acaccgcccc acgcagagca ggaaccgata
     9181 gcgataatgg ctgctgcgcc ttccgccgct ttgcggatgt gatccacaat cggctcaccg
     9241 gcaaccatgc aataaatacc gttatctttt aatgggatgg aaccatccac cactaacaca
     9301 tactgccctt tgtacttctc gagagcgtta tgtttgttct cttcgacctg gtgaccgaaa
     9361 gcagcagaaa gcacctcgtg atactccaga gagatagtct ccagcacgag gttttctacc
     9421 gttggatgcg ttgcacgaag cagagattcc gtacaaccgg tgcactcctg cgcgccaatc
     9481 cagataactg gcggacgctg cgggttagta accgattcgg ccatctctgc agcggctttg
     9541 ctacttaacc ccatggtggc ggctaatgct gcacaaagct tcatgaaatc acgacggtta
     9601 atgccgtgag aatggatgag ggtgttatct ccagtcattt atagttattc cgttgcgaag
     9661 acctggcata tattttgcct caatcgcaaa atcaataatg cgatcgatgc gccatttacc
     9721 acacatttat tatggttatc gatatcatga tactgcggcg gggcgctaaa acgaagggaa
     9781 gaagcattag tgtaattaat taaagcaaga taatacgtat gtttgatcaa ttttcgaggc
     9841 gataaccggc cataaaaaag cgcaacgtag aacaggaatt attctcacga aagaaatcca
     9901 ttaccatctc tttatccagt ccatagcggc gcgtcagaat acccgcttcc caggcgctaa
     9961 gctggcgatc tccggtcttt tcgtacatcc ggtgtgagta gcctaataca aaaccgcgtt
    10021 tataatcggc acagtatttt gtcacattaa ccgcactgtc ggcatgcgtt gcttttaatc
    10081 ccgccattaa gcctttacca aaatggttat tcatcgttca cctcattcgc tatatattgc
    10141 cgtatatagc gatattttag gcaaagaacg acatttgcgc acgctatttt ttcatgcttg
    10201 tttaagacgt aatcctgtaa gagataaacc catcaggaga gatatttatc gaaccatttc
    10261 agcgtccttt gccaggcaag atcggcggca gatttgtcat aacggggcgt ggaatcatta
    10321 tggaatccgt gattaacccc cggatatata tacgcctcat agaccttatt attggctttc
    10381 aacgccgcct cgtaagcagg ccagccctcg ttgattcggg tgtccagttc cgcgtagtgc
    10441 aacagtaaag gcgcttcaat cttcgccaca tcggcagtgg gtgcctgacg accataaaac
    10501 ggcaccgcgc aggccagttc cgggtaagcg accgctgccg cgttcgatac gccaccgcca
    10561 tagcaaaatc cagtaatacc cactttgcct gtcgcttgcg gatagctttg cataaactca
    10621 atcgcggcaa agaaatcatt catcagtttg gttggatcaa cctgctgttg cagctcacga
    10681 cctttatcat cattacccgg ataacctcca acggaactta agccgtcagg tgccagggcg
    10741 atataccccg ccttcgccac tcgccgcgtc acatcttcga tatacggatt cagccctcga
    10801 ttctcatgca ccactaccac ggctggcgtt ttgccgctca tttttgcggg cttcaccaga
    10861 taaccccgta cctcgccgtg accatttggc gagggatacg tgatgtactc tgcaacaatt
    10921 tccgggtcgg taaactctac ctgagtcgcc agcgcataat ttggcttgag caaatcaaac
    10981 agcgctaatg ccgtcatccc gcccaccgca tacttcgccg caaggttgag gaactcacgt
    11041 ttcgagattt tcccgtgagc gtaatagtcg tagtaatcca gcaactcttg tgggaaatct
    11101 ttggcggtta aacgcggcat cgttgcactc ctcaactggt gtttttttaa gcaaagcata
    11161 agcgcgtgtt tttgcccagt ttttcgtcac tctgtgagcc agactacggg atacgcgctg
    11221 gcgaatcgct aaactagaaa cattgtttcg aaattgaacg atggaaagga gaggtcatgg
    11281 tctggttagc gaatcccgaa cgttacgggc agatgcagta ccgctattgc ggaaaaagtg
    11341 gtttacgcct gcccgcgtta tcgctcggtt tatggcacaa tttcggtcac gttaacgcgc
    11401 tggaatcaca gcgtgcgatc ctgcgtaaag cgtttgattt aggcattacg cactttgatt
    11461 tagccaacaa ttacgggccg cctccaggaa gcgcagaaga gaactttggt cgcctgctgc
    11521 gcgaggattt tgccgcttat cgcgatgaac tgattatctc taccaaagcc ggatacgata
    11581 tgtggcccgg cccgtacggc tctggcggct cacgtaaata cctgctcgcc agcctcgacc
    11641 aaagcctgaa gcgtatgggg cttgagtatg tcgatatctt ttactctcat cgcgtcgatg
    11701 aaaatacgcc gatggaagaa accgcctctg cgctggctca tgcggtacaa agcggtaagg
    11761 cgctgtatgt cgggatctcc tcttactcgc cagagcggac gcaaaaaatg gttgagttgc
    11821 tgcacgagtg gaaaattccg ctgttaattc atcaaccttc gtacaattta ctgaaccgct
    11881 gggtggataa aagcggcctg ctggataccc tgcaaaataa cggcgtgggc tgcattgcct
    11941 ttactccact ggctcaggga ttgctgaccg gaaaatatct caacgctatt ccgcaagatt
    12001 cacggatgca tcgtgaaggg aataaagttc gtggtctgac gccgaaaatg ctcaccgaag
    12061 ccaacctcaa cagcctgcgc ttattgcatg aaatggcaca gcagcgtgga caatcaatgg
    12121 cgcaaatggc gttaagctgg ttgctgaaag atgagcgagt gacgtcggta ttgattggag
    12181 ccagccgcgc ggagcaactt gaggagaacg tgcaggcgct gaataatctg acatttagca
    12241 ccgaggagct ggcgcagatt gatcagcata tcgccgatgg agagctgaat ctgtggcagg
    12301 cgtcttccga taaatgacct gttaataacg ggcgcgagaa ccgcgcccga atcagatcag
    12361 tgattatgac gagtcagtcg gtcaagatag cccatcacaa atgcagagag cacaaacgtc
    12421 agatggataa tgacgtacca catcagtttg ttatcaggga catttttcgc atccataaag
    12481 acgcgcagta agtgaatgga agaaattgcc acaatcgacg ctgctacttt gtttttcagc
    12541 gacgttgcgt ccattttccc cagccagttc agcttctctt tgttctcaga gatatcgagt
    12601 tgtgagacga aattctcgta accggaaaac atcaccatca ccagcaaacc gccaaccagc
    12661 gtcatatcca ccagcgacag caacacgagg atcaaatctg attccgccat cgagaagata
    12721 ttcggcagta cgtgaataat ctcctggaag aacttcagcg ccagggcaac taacgccagc
    12781 gaaaggccaa agtacacggg ggcaagcagc cagcgagaag catacattgc attttcaaga
    12841 aaacgttcca tacattcctg tcgtgtagtt aaaacgggca acagtatatc gcaattaagt
    12901 aaacagcacg caacagcaaa tcaacatata cgttaaacct ttgtcgcata attctgaggg
    12961 ttagggatct tcctggttcc cttatcaacg catcgagagc gtctaccctt aaaggacgtt
    13021 gaacctgaag ggagaaaaat gatgtctcat ttaaaagacc cgaccacgca gtattacact
    13081 ggtgaatatc ccaaacagaa acaaccgacg ccaggcatcc aggcgaagat gacaccggta
    13141 ccggattgcg gcgagaaaac ctatgttggt agcggtcgcc tgaaagatcg taaagcactg
    13201 gtgacagggg gcgattccgg aatcggtcgc gctgccgcca ttgcttacgc gcgtgaaggg
    13261 gctgacgtgg cgatcagtta tcttcccgtg gaagaagaag acgctcagga tgtcaaaaag
    13321 atcattgaag aatgcggacg caaagccgtt ctgctgccag gcgatttaag cgatgagaaa
    13381 ttcgcccgtt cgctggttca cgaagcacac aaggcgttag gcgggctgga tattatggcg
    13441 ctggtcgccg ggaaacaggt tgccattact gatattgcag acctcaccag cgaacagttt
    13501 caaaagacct ttgccattaa cgttttcgcg ctgttctggc taacccagga agcgatcccc
    13561 ctgctaccga aaggtgccag tatcatcacc acttcgtcaa tccaggcata ccagccaagc
    13621 ccgcatttac tggactatgc ggctacgaag gcggcaattc tgaactacag ccgtggcctg
    13681 gcaaaacagg tcgcggagaa aggtattcgg gtgaatattg tcgcgccagg cccgatctgg
    13741 acagcactgc aaatttccgg cggacaaacg caggataaga tcccgcagtt tggtcagaaa
    13801 acgccgatga agcgtgcggg gcaaccggcg gaactggccc ctgtatatgt ttatctggca
    13861 agtcaggagg cgagctacgt caccgcagaa gtgcatggcg tgtgcggcgg cgagcattta
    13921 ggctaaaaaa atgcccggtt gtgaaaagca accgggcatc atttttattt cgctttggca
    13981 gtttcttcgc cgaccagacc tatcttcagg taacccgcct gatgcagcgt atccattacc
    14041 ttcatcaacg tctcgtaatc gacggtttta tccgctcgga agaagacggt ggtgtctttc
    14101 ttgccttcgg ttaacgcatt caacgccgta atcattgttt catcggtgac cggatcgtta
    14161 ccgataaaca tcgagttgtc tgccttcacc gacagataaa ccggtttttc cgggcgcggc
    14221 tgcggcgtgc tggtagaagc aggcaagttc accttcacat ctaccgtcgc taacggtgcc
    14281 gccaccataa agataatcag cagaaccaac atcacgtcga taaacggcgt cacgttgata
    14341 tcatgcattt cgccgttatc gtcgaggttt tcgttaagat gcattgccat cggacattat
    14401 cctgcgcgta atttttgtgc gacacgaacc ggatgcgcag cggcgctggc ttccagatcc
    14461 aggtcacggc tttgcagcaa caatacctgc gctgcaacat cacccagcat cgctttaaag
    14521 ccgccaatct ggcgtgcaaa tacgttatag ataacgaccg ccggaatcgc tgccacgagg
    14581 ccgattgccg ttgctaacag agcttctgcg atacccggcg caacgactgc caggttagta
    14641 gtctgcgttt gcgcgatacc aataaagctg ttcatgatgc cccagaccgt accaaacaga
    14701 ccaacgaacg gcgaaatcgc gccgatggtt gccagatagc cgttaccacg gcccatttga
    14761 cgacctactg cggcgacccg acgctccaga cggaagctgg tacgttcttt aataccttcg
    14821 ttatcgtcgc tgccttctga cagttccagc tcattctgcg cttcattgag caaatgcagg
    14881 ctcaagcttt tgctaccaaa atcagcggcg atatcgttgg cctggtttaa ggaacgcgct
    14941 tcagccagca gttgctgctc gcgcttaaga cgacgcttct gattgaagaa ctccacgctc
    15001 ttactgaaga agattgccca ggtgactacg gaggccaaaa taagcccaat catcacgcac
    15061 ttaacgacaa tatcggcgtg ctgatacata ccccagacgg agaggtccgt ctgcattaaa
    15121 ttattaccca ctgtgtatct ccaggacgca agtcacaaaa tctgcgcata ataatatcaa
    15181 aacgacgtcg aattgatagt cgttctcatt actatttgca tactgccgta cctttgcttt
    15241 cttttccttg cgtttacgca gtaaaaaagt caccagcacg ccatttgcga aaattttctg
    15301 ctttatgcca attcttcagg atgcgcccgc gaatattcat gctagtttag acatccagac
    15361 gtataaaaac aggaatcccg acatggcgga caaaaagctt gatactcaac tggtgaatgc
    15421 aggacgcagc aaaaaataca cgctcggtgc ggtaaatagc gtgattcagc gcgcgtcttc
    15481 gctggtcttt gacagtgtgg aagccaaaaa acacgcgaca cgtaatcgcg ccaatggcga
    15541 gttgttctat ggacggcgtg gaacgttaac ccatttctcc ttacaacaag cgatgtgtga
    15601 actggaaggt ggcgcaggct gtgtgctatt tccctgcggg gcggcagcgg ttgctaattc
    15661 cattcttgct tttgtcgaac agggcgatca tgtgttgatg accaacaccg cctatgaacc
    15721 gagtcaggat ttctgtagca aaatcctcag caaactgggc gtaacgacat cgtggtttga
    15781 tccgctgatt ggtgccgata tcgttaagca tctgctgcca aacactaaaa tcgtgtttct
    15841 ggaatcgcca ggttccatca ccatggaagt ccacgatgtt ccggcgattg ttgccgccgt
    15901 acgcagtgtg gtcccggatg ccatcattat gatcgacaac acctgggcag ccggtgtgct
    15961 gtttaaggcg ctggattttg gcatcgatgt ttctattcag gccgccacca agtatctggt
    16021 tgggcattca gatgcgatga ttggcactgc ggtgtgcaat gcccgttgct gggagcagct
    16081 gcgagagaat gcctatctga tgggccagat ggtcgatgcc gataccgcct atataaccag
    16141 ccgtggcctg cgcacattag gtgtgcgttt gcgccaacat catgaaagca gtctgaaagt
    16201 ggctgaatgg ctggcagaac atccccaagt agcgcgagtt aaccaccctg ccctgcctgg
    16261 cagtaaaggc cacgaattct ggaaacgaga ctttacaggc agcagcgggc tattttcctt
    16321 tgtgcttaag aaaaaactca atgatgaaga gctggcgaac tatctggata acttcagttt
    16381 attcagcatg gcctactcgt ggggcgggta tgaatcgttg atcctggcaa atcaaccaga
    16441 acatatcgcc gctattcgcc cacaaggcga gatcgatttt agcggaacct tgattcgcct
    16501 gcatattggt ctggaagatg tcgacgatct gattgccgat ctggacgccg gttttgcgcg
    16561 aattgtataa cattgccact tttggacaat tttgcagaca ttttattgtg aaaagtctta
    16621 aattgttgcg tccgggatca aggcgtcccg gacgattcag gagtacaata ggcagataaa
    16681 ggcttaaacg ctgttccaca ggaaagtcca tggctgttat tcaagatatc atcgctgcgc
    16741 tctggcaaca cgactttgcc gcgctggcgg accctcatat tgttagcgtt gtttactttg
    16801 ttatgtttgc cacgctgttt ttagaaaacg gcttgctgcc cgcctcattt ttgccaggcg
    16861 acagcttgtt gatactggca ggcgcattga ttgcccaggg ggttatggat tttctgccta
    16921 cgattgcgat tctgaccgcc gcagcaagtc tgggctgctg gctaagttat attcaggggc
    16981 gctggttagg gaataccaaa acggtgaaag gctggctggc acagcttcct gctaaatatc
    17041 accagcgcgc cacctgcatg tttgaccgcc acggtctgct ggcgctgctg gctggacgtt
    17101 ttcttgcatt tgtccgtacg ctgctgccaa ccatggcggg aatttccggt ctgccaaacc
    17161 gccgcttcca gtttttcaac tggttaagcg gattgctgtg ggtcagcgtg gtaaccagtt
    17221 tcggctatgc cttaagtatg attccgttcg ttaaacgcca tgaagatcag gtaatgacgt
    17281 tcctgatgat cctgccaatt gccttgttaa ccgctggctt gttgggcacg ctgtttgtgg
    17341 tgattaaaaa aaaatactgt aacgcctgac gattttcccc attcccggta gctgtaccgg
    17401 gaacgtattt aattcccctg catcgcccgc attcttgccg catcttcccc cggcgtcaca
    17461 ccgaagtaac gtttaaactc acggctaaat tgcgatgcgc tttcatagcc gacgcgcatc
    17521 gccgccgcgc tggccttcat gccgtcgtgg atgatcatca tccgcgcctt atgcagacgg
    17581 taattcttca aatactgcaa cggcgaggtg ctggtgacag acttaaaatt atggtggaac
    17641 gccgatacgc tcatgttggc ttctgccgcc agttgctcga cgctcaggtt ttcggtgtat
    17701 ttattctcaa tccgtttcag cacgcgacta atcaggctga agtgagtctg gcggctgacc
    17761 agtgccagta acgcgccgcc gcaaggtccg gtcagcacgt agtacagaat ttcgcggatg
    17821 atctgtttgc cgagaatacg cgcatccagc ggtcgctcca tcacatccag taaccgctcc
    17881 gccgcgcata aaatctcttc tgataacgtg gcagagttaa tcccgctggc tgccatcgac
    17941 ggctggaaat gctcatcttc gccaatgtcc atcaacagtt cctgtaactg caaaatatcg
    18001 acattgagac gcaaccctgc cagcggcacc tctgacgtgg cataggtttc gcactcaaac
    18061 ggcaacggca ccgtcagcag gagatattca ttagcgtcat aacgaaacac gcgttcattg
    18121 atataaccga ttttatgtcc ggaaaagaga attatgatgc caggctcgta catcaccggt
    18181 gtgcgtgcga aaggcgtctc gccatacaac aaacgcacat cgggcaacag ctctgacaaa
    18241 ctattttctt tatttttcag tttattaact ttatccgcca gcaagcggca aatctcttca
    18301 cgtttcatat cgcgtaattt cttaggaata atgcggcaat ttgattgtgc gcaattttgt
    18361 agcatttctc cagcactctg gagaaatagg caagacattg gcagaaatga gcattgagag
    18421 gtacggcgct ggcgaccaca atgaaaaaca tcaggcagac cgttctctgc cctcatattg
    18481 acccagcaaa gggagcaagt aatgaacaac tttaatctgc acaccccaac ccgcattctt
    18541 tttggtaaag gcgcaatcgc tggtttacgc gaacaaattc ctcacgatgc tcgcgtattg
    18601 attacctacg gcggcggcag cgtgaaaaaa accggcgttc tcgatcaagt tctggatgcc
    18661 ctgaaaggca tggacgtgct ggaatttggc ggtattgaac caaacccggc ttatgaaacg
    18721 ctgatgaacg ccgtgaaact ggttcgcgaa caaaaagtga cttttctgct ggcggttggc
    18781 ggcggttctg tactggacgg caccaaattt atcgccgcag cggctaacta tccggaaaat
    18841 atcgatccgt ggcacattct gcaaacgggc ggcaaagaga ttaaaagcgc catcccgatg
    18901 ggctgtgtgc tgacgctgcc agcaaccggt tcagaatcca acgcaggcgc ggtgatctcc
    18961 cgtaaaacca caggcgacaa gcaggcgttc cattctgccc atgttcagcc cgtatttgcc
    19021 gtgctcgatc cggtttatac ctacaccctg ccgtcgcgtc aggtggctaa cggcgtagtg
    19081 gacgcctttg tacacaccgt ggaacagtat gttaccaaac cggttgatgc caaaattcag
    19141 gaccgtttcg cagaaggcat tttgctgacg ctgatcgaag atggtccgaa agccctgaaa
    19201 gagccagaaa actacgatgt gcgcgccaac gtcatgtggg cggcgactca ggcgctgaac
    19261 ggtttgatcg gcgctggcgt accgcaggac tgggcaacgc atatgctggg ccacgaactg
    19321 actgcgatgc acggtctgga tcacgcgcaa acactggcta tcgtcctgcc tgcactgtgg
    19381 aatgaaaaac gcgataccaa gcgcgctaag ctgctgcaat atgctgaacg cgtctggaac
    19441 atcactgaag gttcagacga tgagcgtatt gacgccgcga ttgccgcaac ccgcaatttc
    19501 tttgagcaat taggcgtgcc gacccacctc tccgactacg gtctggacgg cagctccatc
    19561 ccggctttgc tgaaaaaact ggaagagcac ggcatgaccc aactgggcga aaatcatgac
    19621 attacgctgg atgtcagccg ccgtatatac gaagccgccc gctaagcttt ttacgcctca
    19681 aactttcgtt ttcgggcatt tcgtccagac ttaagttcac aacacctcac cggagcctgc
    19741 tccggtgagt tcatataaag gaggaacgta tggctaatcc aaccgttatt aagctacagg
    19801 atggcaatgt catgccccag ctgggactgg gcgtctggca agcaagtaat gaggaagtaa
    19861 tcaccgccat tcaaaaagcg ttagaagtgg gttatcgctc gattgatacc gccgcagcct
    19921 acaagaacga agaaggtgtc ggcaaagccc tgaaaaatgc ctcagtcaac agagaagaac
    19981 tgttcatcac cactaagctg tggaacgacg accacaagcg cccccgcgaa gccctgctcg
    20041 acagcctgaa aaaactccag cttgattata tcgacctcta tttaatgcac tggcccgttc
    20101 ccgctatcga ccattatgtc gaagcatgga aaggcatgat cgaattgcaa aaagagggat
    20161 taatcaaaag catcggcgtg tgcaacttcc agatccatca cctgcaacgc ctgattgatg
    20221 aaactggcgt gacgcctgtg ataaaccaga tcgaacttca cccgctgatg caacaacgcc
    20281 agctacatgc ctggaacgcg acacacaaaa tccagaccga atcctggagc ccattagcgc
    20341 aaggagggaa aggcgttttc gatcagaaag tcattcgcga tctggcagat aaatacggca
    20401 aaacccctgc gcagattgtt atccgctggc atctggatag cggcctggtg gtgatcccaa
    20461 aatcggtcac accttcacgc attgtcgaaa actttgatgt ctgggatttc cgtctcgaca
    20521 aagacgaact tggcgaaatt gcaaaactcg atcagggcaa gcgtctcggt cccgatcctg
    20581 accagttcgg cggctaacgt gcaaattatc ccggtggcgg taatgttccg ctaccggact
    20641 tttcagaaat catttattcc cctcgcgtcc cgcccgttgc tactcttcct tgttcaggaa
    20701 tgccaaatat aaggacatca tcatgcagag ccggaagctc ttaaaagaac aactcatcta
    20761 tatccgggat aaacgcaacg gagaggtgaa aaacagatga aaataatact tctgttttta
    20821 gcagccctgg caagttttac cgtacacgca cagcccccct cacagaccgt agaacaaaca
    20881 gtccggcata tttatcagaa ctataaatca gatgccactg ccccttattt tggtgaaacc
    20941 ggagagcgag cgataacttc tgcgcgtatt caacaggcgc ttaccctgaa cgacaatctt
    21001 acgctgccgg gcaatattgg ctggctggat tatgatccgg tttgtgattg tcaggatttt
    21061 ggcgatctgg tgttagaaag cgtagcgata acccaaactg acgccgatca tgccgatgcc
    21121 gttgtgcact ttcgtatctt taaagacgat aaagaaaaga cctcgcagac actgaaaatg
    21181 gtggcggaaa atggtcgttg ggtcattgac gatattgtca gcaatcatgg cagcgtctta
    21241 caagcggtta atagcgagaa tgaaaaaaca ctggccgcta tagcttcgtt gcaaaaagaa
    21301 cagccggaag cctttgttgc cgaactcttt gaacatattg ctgattatag ctggccgtgg
    21361 acgtgggtgg tttccgactc ttaccgccag gcggtcaatg ccttctataa aaccaccttc
    21421 aagacggcca acaatcccga tgaagatatg caaatagaac ggcaatttat ttacgacaat
    21481 ccgatctgtt ttggcgaaga gtcgttattt tcacgcgttg atgaaattcg agtcctggag
    21541 aaaaccaccg attccgcccg cattcatgtt cgttttacgc tgaccaatgg caacaacgaa
    21601 gagcaagaac tgattttaca acggcgcgaa ggtaagtggg aaatcgctga ttttatccac
    21661 ccgaacagcg gcagcctact taaacagatt gaggcaaaaa ccgccgccag attaaaacaa
    21721 tgagctgaat taaataacaa ttagccggaa caataaataa aagggaacac tatatgaaaa
    21781 cgattttcac cgtgggagct gttgttctgg caacctgctt gctcagtggc tgcgtcaatg
    21841 agcaaaaggt caatcagctg gcgagcaatg tgcaaacatt aaatgccaaa atcgcccggc
    21901 ttgagcagga tatgaaagca ttacgcccac aaatctatgc tgccaaatcc gaagctaaca
    21961 gagccaatac gcgtcttgat gcacaggact atttcgactg cctgcgctgc ttgcgtatgt
    22021 acgcagaatg ataaaaaaat ccccggcagc attctgttac cggggatatt ttttaacgtc
    22081 caaccgccgc tttagggcgt ttcttcgcac cagcattctg caggcgagat tgcgtagacg
    22141 acgctttttt tgccgtagca ggcgtctgac gctgggtcgc catcggcgta tgtttagtca
    22201 acgccggaag ggtattgcgg ttctggcgac gagcctcacg catctcttca atggttggcg
    22261 caggcactaa gcaatcgcga cggctgccaa tcagatgctt tttacccatt gcttccagcg
    22321 cctggcggat taacggccag tttgccggat cgtggtaacg caacaacgct ttatgcaggc
    22381 gacgctgctt gtcgcccttc ggtacgaaga cgtcttcact cttataacca atcttcgcca
    22441 gcgggttttt gccggtgtaa tacatggtgg tcgagttagc cagcggcgac ggatagaagt
    22501 tctgcacctg gtcgaggcgg aagcgatgct ttttcagcca tagcgccaga ttcaccatat
    22561 cttcatcacg cgtacccggg tgcgcggaga tgaaatacgg gatcagatac tgttctttac
    22621 ctgcctgttt cgagtaagta tcgaacagct ctttaaagcg gtcatagctg cccatgcccg
    22681 gcttcatcat cttcgataac ggcccttctt cggtatgttc cggggcaatc ttcagataac
    22741 cgccgacgtg atgggtcgcc agctctttga tatagcgcgg atcttctacg gcgatgtcat
    22801 aacgcacacc agaggcaatc aggatctttt taatgccttt cagatcgcgc gcacggcgat
    22861 agaggttgat cgtcggttcg tggttagtgt ccatatgcgg acaaatatcc ggataaacgc
    22921 acgacaaacg acggcaggtt tgctcagcgc gtggcgattt gcaacgcaac atatacatgt
    22981 tggcagtagg cccaccgaga tcggaaatca cgcccgtaaa acctggaaca gtgtcgcgga
    23041 tcgcttcgat ctcattaata atcgaatctt cggaacggct ctgaataatg cgcccttcgt
    23101 gctcggtaat agaacagaaa gagcagccgc caaagcagcc acgcataatg ttgaccgaga
    23161 aacgaatcat ttcgtaagcc ggaattcggg cattaccgta agccggatgc ggcacgcgct
    23221 tgtacggcag cgcaaagacg ctatccatct cttcggtaga aagcggaata gcaggcgggt
    23281 tgatccatac atagcggtcg ccatgttttt gcatcaatgc gcgggcgcag cctgggttag
    23341 tttcgtggtg cagaatgcgc gaagcatggg cgtacagcac tttatcgccc ttcactttct
    23401 cgaaagaagg cagcaacacg taggtttttt cccacggttt cgggcgtggt ggctgcacgg
    23461 ttacagcttt ggcttcctgc tttttcggag ctaccggttt gttatccgcg cacggcaaat
    23521 cttcgccata cggatgcggg attgggtcga ttttccctgg ggtatcaaga cgggtggaat
    23581 ccacaccgct ccagccaggc aacgcctctt ttacgataat cgcggtatta cgcacatcgc
    23641 ggatttcact aatcggctcg cccatcgcca gacggtgcgc cacttccacc agcggacgct
    23701 cgccattgcc aaacatcaac atgtcggctt tagaatccac cagcacggaa cggcgcacgg
    23761 tatcggacca gtaatcataa tgcgcggtac gccgcaggct ggcttcgata ccgccgagga
    23821 tcaccggcac atctttccac gcctctttac aacgctgggt ataaaccagt gttgcacgat
    23881 ccgggcgctt acccgcgacg ttattcggcg tgtaagcgtc atcatggcgc aaacggcgat
    23941 cggctgtgta acggttgatc atcgagtcca tgttgccagc agtaacgccg aaaaacagat
    24001 tcggtttacc cagacgcata aagtcgtctt tgctgctcca gtctggctgg gcgatgatcc
    24061 caacgcgaaa gccctgcgct tccagcatac gaccacaaat cgccatcccg aagcttgggt
    24121 gatcgacata cgcgtcgcca gtaaccaaaa tgatgtcgca gctatcccag ccaagttgat
    24181 ccatctcttc acgagacatc ggcaaaaacg gtgccggtcc aaaacaggcg gcccagtact
    24241 gcggccagga gaacaggtcg cgatccggtt ggatcaggga gatagagctc attttgcttc
    24301 cagaaatgat aaaaaaataa tcaaaggccg gggattataa gccggaacga aagagaaatc
    24361 gaaaggtatt ccatactcgc cctcctcggg cgagtatgaa gattacggta ccggattgac
    24421 taacagttgc ccaatcgagc cacggtccgc catttccagc gtctgactgt tgaagtagaa
    24481 cgggaagtgc gcccaggaag gctgaccgaa ataaacaagc agctccactt gtccgtcaac
    24541 ccaaacggta tctttccagc ctctgtcttc cgggaacggc atcgcgccat tcacgttacg
    24601 gatctggaac atcacgcctt caatatggaa cgcttgcggc tcgtccgcgc gcaccgtcca
    24661 gcgttcccac gttccttgct gcgcggtgac atcaatacgg ttgacgtccc acagttgtcc
    24721 attaataccc gggtcatcac ccagactaat atcgcgactg cgaatcggcg aaccggccat
    24781 gatttcagtt ggcagcaagc gcatcggaag actgtcagtg accagcggca gaaggccggt
    24841 tgggcgcagc gttagcacca gcgtagaaac cagaatgctg gatggctcaa agaagccacg
    24901 aatacgatca acaatgctcg ccgcttcgcc acaggtaatc gacacttcat caccgttgct
    24961 catatccacc agaatctcac gacgctcgcc cggtgccagc gaaagttgct tcaccgatac
    25021 aggagcgggc aggaatccct gatcgccaga aatcacatgt aacgggcgac catcgctcat
    25081 ctgtagttga tagcgacgag agttcgacgc gttcaacaga cgcaagcgca cccagccacg
    25141 cgagacttca acgtacgggc tttgtacacc gttaaccagc agcgtatcac caacaaagcc
    25201 gccgcttccc ggttcgttgt attctggcgt accaaagtta tccagccgtt tatcctgaat
    25261 aatgacagga aaatcatcca caccataatg gttggggata ggcagcgatt tactgacttc
    25321 atcttccacc agccacattc cggcaaggcc gttatagacc tgctgagccg tgcggttggg
    25381 agtattggcg tgataccaca gagtagctgc gttctggcga atgggcagta cgggtgccca
    25441 gtcagcgttt ggcgacatca tccgtgccgg accgcccatc agcgggccag gtacctgtag
    25501 cccggccacc gtcattgaga cattttctgt caggcggttg ctgtaaataa gcttaacatc
    25561 gtcgcccttc cagacgcgga tagtcggccc caggtaacga ccattgattc cccaaaccga
    25621 cgcgcgtgtc cctggcgtaa atgaccagtg cgcacgttgt acagtcataa acagcggttg
    25681 cccacggcga gattcaagca gcggcggaac gggtagcggt tgctgttgcc cggctgcgct
    25741 ggccttcagg ggaacagcgc ctgcacaaag tgcaatcccc gatgcctgaa tgaactgacg
    25801 ccgactgagt gacatatttg ctccatgtaa aactggctaa agtaataccg tgcaggaaag
    25861 tgttccccgc aaaaatacag attgccgact taaacttttc cggcggcttc gcgttctgcg
    25921 acttctttat cgagctcggc gattttttgt tccattatcg aacgacaatg ggcagccaac
    25981 tcgcgaacct gatctttgcc atactgactg acgtcaattg gcggcagcat ttcgacaatc
    26041 accagaccgt tgtgtagccg attaagatta atcttattcg aagttgtaga gacgcacacg
    26101 ggtataatcg ggacgcccgc cgcaattgcc gcgtgaaatg ccccggtctt gaacggcagc
    26161 aggccgcgac cacggctgcg ggttccttcc gggaacatcc agatggaaat acggcgtttt
    26221 ttgaagtgat tcactacttc cgcaatagtg ccgtgagctt tagtgcgatt gtttctgtcg
    26281 atcaataagt tgccggttaa ccagtacaac tgcccgaaga aggggatcca cagcaagctc
    26341 tttttaccta ccgtcactgt cggcggctgc acaatgttcg atgccgtcac catgtcataa
    26401 ttgttctggt ggttagcaat atagatagca ttgccgtagc tttcagcgtc tgcaggtttg
    26461 cggcactcca ctttcaggcc gaaaagcggc gcaagacggc caaacatatg cccaaaggtg
    26521 gccacatgtt tcgggttacg cgggctgaaa aggcagtaaa tggagccgaa tacacagact
    26581 aagatgctgt aaatcacggt aataataaga cgaaagatat atagcataac accctctgaa
    26641 ggtctgagag actggcattg tacgtcacct gaaaatcagg ttagggcatt gttaaaagcc
    26701 gctctctgga aaagcgtatt gttaatcgca acgcatgaat aaacaacggt ttcaagggaa
    26761 tttttatcgt ttccggggat tgaatacgct gccggatgac gacttaacgt ttcatccggc
    26821 gttccttgca agcgggagga aacagcgccc tccccggcat attactcttc gctatcaccg
    26881 ctgctggcac ggcgaggaga gtcgatctca acacgatcga tacgctgcaa accgcgcatc
    26941 aacgtaccgc ggcgtccacg ttcgccagcg actttctgta gctcttccgg acgcagttta
    27001 attttgcgtt tcccaacatg aatggtcagc gtgctttgcg gcggcagaac gtacagttgc
    27061 gcaagaccat cctcgccacg cgcggcttct gccgatggaa tgttgataat cttgttgcct
    27121 ttgcccttcg acagctgcgg cagatcgctt accgggaaca tcaacatacg gcctgcctga
    27181 gtgattgcca gcagcatatc ggaagcatct tcaatcacca ccggcggcat aacatgggca
    27241 ttttccggta aggtgatcaa agccttacct gcacggttac gcgccaccag atcgctaaag
    27301 gtgcagacga aaccgtaacc cgcatcggaa gccatcagca gtttctgatc gtcgctttcc
    27361 atcagcatat ggtcaacggt cgctccaggc ggcaacgtta atttgccggt gagcggctcg
    27421 ccctgaccac gcgccgacgg cagcgtaatc gggtcgatgg catagctacg accggtggaa
    27481 tcaacaaaca ctaccggttg gttgctctta cctttcaccg ccgctttgaa gctatcaccc
    27541 gctttataat tcaggcccgg cgcgtcgata tcatggcctt tagcgctgcg tacccagccc
    27601 atctgcgaca gcacaatggt gacaggttct gacggcagca tgtcgtgctc gctcatcgct
    27661 ttcgcttctt cgcgttcctg caacggcgaa cgacgttcgt caccgtaggc ttgcgcgtct
    27721 gcctgcagtt ctttcttcag caggttattc attttacgct cggaagccaa aatgccctgc
    27781 aactggtcgc gctctttttc cagctcactc tgctcaccgc gaatcttcat ctcttccagt
    27841 ttggcaagat gacgcagttt cagttcgagg atcgcttccg cctgggtttc cgtaaggcca
    27901 aaccgcgaca tcagcgccgg tttcggttca tcttcattac gaatgatctc aatcacttcg
    27961 tcgatattga gaaacgccac cagcaaacct tcgaggatat gcaagcgctt gaggactttc
    28021 tccagacgat agttcagtcg gcggcgcacg gtatcgcggc ggaacaccag ccattcggag
    28081 aggatttcca gcaggttttt caccgccgga cgaccatcca gaccgatcat attgaggtta
    28141 atgcgatagc tcttttccag atcggtggta gcaaagaggt ggttcatcac ctgatccata
    28201 tccacgcggt tggaacgcgg cacaatcacc agacgggtcg ggttctcgtg gtcagattca
    28261 tcgcgcagat cgtcaaccat cggcagcttt ttgttgcgca tttgcgcagc aatttgctcc
    28321 agtacgcgcg cacctgaaac ctgatgcggc aatgcgctga taaccaccgc gccatcttct
    28381 ttcttccaca ccgcgcgcat acgcactgaa ccacgtccgt tctcgtagat tttacgaatc
    28441 tcggcgcgcg aagtgataat ttccgcttca gtcggataat ccggcccctg cacgatatcc
    28501 agcagctgat cgagagtggt tttcggctgg tcgattaatg cgattgccgc ctgagccact
    28561 tcacgcaggt tatgcggtgg aatatcggtc gccatgccga cggcaatacc ggtggtgccg
    28621 ttaagcaaaa tgtttggcag acgggcaggt agcattttcg gctcctgcaa agtgccgtcg
    28681 aagtttggga cccagtcagc cgtcccctgc cccagctcgc tcaatagcag ctcggaatat
    28741 ttcgacaacc gggattcggt gtaacgcatt gccgcgaacg atttcggatc gtccggcgca
    28801 ccccagttcc cctgaccatc aaccagcgga taacggtaag agaacggctg cgccatcagg
    28861 accatcgctt cataacaggc actatcgccg tgcggatggt atttacccag tacgtcaccg
    28921 acggtacggg ccgatttttt aaatttggcg ctggcattca ggcccagctc agacatcgca
    28981 tacacaatgc ggcgctgaac aggtttcaga ccatcaccaa taaacggcaa cgcacggtcc
    29041 atgatcacgt acatggagta gtttaagtag gcgttttccg taaattcatg tagcgcaagg
    29101 cgctctgcca tatcgctcat taattctgat tcctcaactt attcgccagc cttcaaaggg
    29161 gcaatattgc cggagatact acattatctg ccgcgtcgag tcacaggaga tcagcgtgaa
    29221 tagcgccgcc ggacgtgctc cggcggcgat aaaggcatca atgtgccttg atatacaact
    29281 ctttgctgta gacataatcc tggggattat gcagcggaaa accgccaacg tagggtttaa
    29341 gcagtttgat taacggctga tagtagatgg gaatcagcgg tgcctgctgg ttgatgatca
    29401 cttctgcctg ctgatacagc gcattacgct ttgttgcatc agccgtttgt gccgcctggt
    29461 ttagtaaggc gtcatactgc gcgtttttcc agtggccgac gttttcttca ctatcgcttt
    29521 tgagcgtgtt caggaagctg gaggcatcat tgtacgtcgc atcccacgac tgccaagaca
    29581 gcatgaaatc accggctcgt cgggcatcaa gataggtttt ccactccatt gtgcgcagcg
    29641 tcacctgtgc acccagccat tttttccatt tggaagacaa cgctatcgcg gtcttttcat
    29701 gcagatcgta cttgttgtag aacagctcaa agcgtagcgg atgagaggcg tcgtatcccg
    29761 cctgtttcag caagactttt gccatcgcga cacgctcact cattggcttt tgcagttcat
    29821 cgaacgtcgt cgcgctaaag ccttttacct ctggcggcgt cagcgtggtt gcgggcgttc
    29881 tcaaccccag taccttttgc gcaataagct gtcgatcaac cgtaagatat agcgcccgac
    29941 gcacccgcac atcgttaaat ggcggtttct caaggttaaa gttgtaatat tcgctgttca
    30001 gacgcgggat aattcgtagc tcgccaggca gtgatttttc aatggcggga atttgctgcg
    30061 ccggaaccca ggtgagatcg acctctcccg cgcgatagcg gttatagccg gtgaccgaat
    30121 tatccagcgc cagatactca acctgttgca atactgtatg ttgcgcatcg cggtactttg
    30181 gatttttgcg tgcagtaatc ttttcgttaa ctacccattt atcaagcaca aaggcaccgt
    30241 tgtaaaccat gttctctggc ttactccagc tatcgccatg tttagcgatg acatgatgag
    30301 gaaccgggaa cagcgtcggc caggcgagca tcgtcgtgaa ccaaggaact ggctgctcaa
    30361 gcgtaacttc aagagtacga tcatccgtcg ctttgacacc cagcgatgta acatctgctt
    30421 tgcccgcaac aatagccgcg gcattgttaa tgtgcgcctg tgccagatag ccagcaaaag
    30481 ggcttgccgt tttcgggtca accgcgcgct gccagccgag gacaaaatcc tctgccgtca
    30541 gaggctgacc gtctgaccac tgcaaaccgc tacgcagatg gaaaatatag cgcttgccgc
    30601 cgtcaagtat ctcccagcgt tcagcctgag cgggctgcac ctggccttca ccgtccatcc
    30661 ataccagacc ttcaaacaga tccagcacaa tctgcgcggc ggtattctcc tctacctttt
    30721 gcgggtcgag cgtgcctggg tcgctatgat tgttgtaacg aaagacttgt tgcggggcga
    30781 gcggtgtgtt ggcgggaacg tcagcagcgt agagaggagc cgcacttacc aggctgacca
    30841 gccataataa atttcgcgta tacatggtat tccttaccgg agaaattata agcccgacaa
    30901 caatacgcct gtggcattgt ttgctcaaac ttatcgcgag tgatttggct cacactccgg
    30961 taaagattcc ccatcacgct aacggtacat aaacatccgt ttccagtcgc tgctctgtca
    31021 tcccttcggc aagattggtg tagtgaaaca gaatcggtgc tttacgcatt ttctcgccgc
    31081 ttgcaggcag ccagtggcga atgatgcccc atatcgtgtg tgaaatatcg tccagctcgc
    31141 caacatggcg ggccacagca taacgtccac cggtaagttc accattactt acgccataac
    31201 gattatcggg gatcggttcg ctaacgctgc cgcagatatc aaagcgaaac gcttccggtg
    31261 cggtggtggc agggtcatcc caggcgacgc caaaagtctg gctttggtta acaggagaaa
    31321 gccccgtctc tttacgccac atgataaatt tcgccacgct gtagttgagc aactcagggc
    31381 tacagcggtg cggcaacatc gccacaggga ttgatgggaa atcgataata tttacatcca
    31441 gtgtcaggtt tgtcattttg cctccctgcg aaattattcc cgggaacgcg tcccgggagc
    31501 ggtaacaatt acggatttac tttgcggatc tgtttgacgt caatttcaac agagttccag
    31561 tctttatcga cttcaccctg aatctcaacc gtatctttcg gcgtcaccgt cacgccgttc
    31621 cagcgtttat ggtcgatatc aacattgata gtaccgctgg catctttgaa cacgtagaga
    31681 tcgtcagaga tgcgttcaac gatattgccg cgtagggtta cccaggtgtc gtcacgcagg
    31741 gattttgcgc tttctacagt cgttacgctg ccgttcggcc cctggaatcc tccggcctga
    31801 ctttgcgttg ccgatgggcc agaaaaacct ccctgctcag ctgccataac cggtgcactg
    31861 cacagggcca ttactgcgat tactgctgcg aattttttca tgtttattac tccctttaat
    31921 gtctgtttcc gagcatttaa caagatagtc cttaacaact tcttaaggga aaaaaataaa
    31981 atttaatgct gtacagagcg cgttacaaca cggtttactg gcagcaaata cggttatcgc
    32041 agggatgaaa aaatgcgaat tttactgata gaagatgaca tgctgattgg cgacggcatc
    32101 aaaacgggcc ttagtaaaat gggttttagc gtcgactggt ttacacaagg tcgtcaggga
    32161 aaagaggcgc tatatagcgc accttatgat gcggtgatcc tggatttaac cttaccaggc
    32221 atggatggtc gcgatatttt gcgcgaatgg cgagaaaaag gtcagcgtga gcctgtattg
    32281 atcctgactg cgcgcgatgc gctggcggaa cgtgtagaag ggctgcgtct gggagctgac
    32341 gattatctgt gtaaaccttt tgcgttgata gaagtcgccg ccaggctgga agctctgatg
    32401 cgccgaacca acggccaggc cagcaacgag ctgcgccacg gcaacgtcat gctcgacccc
    32461 ggcaaacgta tcgccacgct ggctggcgaa cccttaacgc tgaaaccaaa agaatttgcc
    32521 ctgctggaat tactgatgcg taacgctggt cgggtactgc cgcgcaaact gattgaagag
    32581 aaactgtata cctgggacga agaggtcacc agtaatgccg ttgaagtgca tgtgcatcat
    32641 ctgcgacgca aactcggtag tgattttatt cgtaccgtgc atggtattgg ctacacatta
    32701 ggtgagaaat gaaatttacc caacgtctta gtctgcgagt caggctgacg ctaatctttt
    32761 taattctggc ctcggtgacc tggctgcttt ccagctttgt cgcctggaaa caaacaacgg
    32821 ataacgtcga tgagttgttc gacacccaac tgatgctgtt tgccaaacgg ttaagtacgc
    32881 tcgatctcaa cgaaatcaac gcggcggatc gcatggcaca gacgccaaat aaattaaaac
    32941 acggtcatgt tgatgacgat gcgctgacct ttgccatctt tacccacgac ggcagaatgg
    33001 tccttaacga tggcgataac ggagaagata ttccttatag ctatcagcgg gaaggttttg
    33061 ctgacgggca actggtcggt gaagacgatc cctggcgttt tgtctggatg acctcacctg
    33121 atggcaaata tcgcatcgtt gtgggtcagg aatgggaata ccgtgaagac atggcgctgg
    33181 cgattgttgc cggacaattg atcccgtggc tggtcgcact gccgattatg ttaatcatca
    33241 tgatggtact actgggtcgt gaactcgcgc cgctgaacaa actggcgctg gcactacgta
    33301 tgcgtgaccc agactcggaa aaaccactaa acgcgacggg cgtacccagc gaagtgcgcc
    33361 cactggttga atcgctaaat caactgttcg cccgcacaca tgcgatgatg gttcgtgaac
    33421 gacgctttac ctccgacgca gctcacgaac ttcgtagccc gttaacggcg ctgaaagtgc
    33481 aaaccgaagt tgctcagctc tctgacgatg atccgcaggc gcggaaaaaa gcgctgctcc
    33541 aattacattc cgggatcgat cgcgctactc gtctggttga tcaactgctc acgctatcgc
    33601 ggctggactc actggataac cttcaggacg tcgcggagat cccgcttgaa gatctcctgc
    33661 aatcgtcggt gatggatatt taccacacgg cgcagcaggc gaaaattgac gtgcgactga
    33721 cactcaatgc caacggcatc aaacgcaccg gacaaccgct attgctaagt ttgttggtgc
    33781 gaaatttgct ggataacgcc gtgcgttaca gtccacaagg tagcgtggta gacgtcacgc
    33841 tgaatgccga taatttcatc gtgagggata acggccccgg tgtgacgcca gaggcactgg
    33901 cgcgaattgg cgaacgcttc tatcgcccac ccggacaaac cgctaccggc agtggacttg
    33961 ggctatcgat tgtccagcga atcgccaaat tgcatggcat gaatgttgat tttgggaatg
    34021 cggaacaagg tggatttgag gcgaaggtaa gctggtaagg gtaagacttt tgctaaattc
    34081 gcaaaagact ttgcacattt tgctaatttc accataccgc tctgtgacgt actatagtcg
    34141 gcaaacgtct caccttgagg ttaaaaaatg agcaacatcc tgattatcaa cggcgcgaaa
    34201 aaattcgccc actccaatgg tcaactgaac gacaccctga ccgaagtcgc ggatggcaca
    34261 ctgcgcgacc tcgggcatga tgtccgcatc gttcgcgccg acagcgacta cgatgtcaaa
    34321 gcggaagtac aaaactttct ctgggctgat gtggtgatct ggcagatgcc tggctggtgg
    34381 atgggcgcgc cgtggacagt gaaaaaatat attgatgatg tattcaccga aggtcacggg
    34441 actctgtatg ccagcgatgg tcgtacccgc aaagatccat caaaaaaata cggttccggc
    34501 ggcctggtac agggcaaaaa atatatgctt tctctgacct ggaacgcacc aatggaagcc
    34561 ttcaccgaaa aagatcagtt cttccacggc gttggtgttg acggtgtgta cctgccgttc
    34621 cataaagcaa accaattcct tggcatggaa ccgctgccga catttatcgc taatgacgtg
    34681 ataaaaatgc ctgatgttcc ccgctatact gaagaatatc gcaagcatct tgtggaaatt
    34741 tttggttaac tagagctctg gctttagaag gagttaacca tgattacagt aatcgcagaa
    34801 atccgtactc gtcctggtca acatcaccgt caggcagtgt tggatcagtt tgctaaaatc
    34861 gttccaaccg tactgaaaga agaaggttgc cacggctatg cgccaatggt ggattgcgct
    34921 gctggcgtga gtttccagtc tatggcaccg gattctatcg tgatgattga gcagtgggaa
    34981 agcatcgcgc atcttgaagc gcatctgcaa accccgcaca tgaaagcata tagcgaagcc
    35041 gtaaaaggtg acgtgctgga gatgaatatt cgtatcctgc agccggggct ttgacatttc
    35101 taaaaagagg ccggtgctgc taccggtctc agcccagatt agccattctt tcttcccgtt
    35161 ttcagtcctc gatggaaatc gttaattcgc ttaatggttt taaccgcacg ctgcggcact
    35221 gcggaagcta ccgaccagac aatcatttcc agacacaaca ataccgtacc atgtagcggc
    35281 gttttccctt tttcatcccc gcgcggaaca tgaatcacga tactggcgtc tttgctaaaa
    35341 cgtgaatcca gcgcgtttgt cagcaaaata acagggatcc ccagccgcct cgcctcacga
    35401 agcgttgtca gcccttcccg gtgcgcagat ttttgcgcca tcatgatcag aacatcgcca
    35461 cgttgaagcg cgatcagttg ctcggcaagg ccaatacccg tacggtttag tgccgtggca
    35521 ggcaaaccta tgcggttgaa tagtcgtgcg gtatagtccg ccagaatacc cgaagcgcca
    35581 atgccaaaaa ttgcgacttg ccgtgcctgc accaacaacg ctacagcttg cgcaagcgcg
    35641 tggcggtttt caggctctga caggacatta caggtataca aatgcccttc aagaacaaag
    35701 tcgattgcgg tgttaacatc gctcgtcaag ttactcaccg tcgtagacat cttttcgctg
    35761 gaactaaggg ctggaccaag ccactgttcc agagtgcgtt tcagatcacg cagcccggca
    35821 aaccccaggg cctgtatagc cctgataact gtggcatcgg aagtgttcaa cgtggccgca
    35881 atttccatcg ccgtttgctc gagtaccact tcacgatgct cattaatgta gctggcgaca
    35941 actcttaacc caggcgttaa ctgactcgcg cgagtgcgaa aacgctcacc aaaaatatca
    36001 acccgcactt tatctttctg gatcatttag cctccggtag aggtcgccct gcaatttgtg
    36061 attgtatctg cgcagccatc agccccagag atgcaaagga gttagaaatt gcctcttcac
    36121 gagaaatcag cacataacgc ccggaacgac aggcagtcag aaactgacac catcccggca
    36181 tgactttttc cattgctgcc agctcatcct gaggtttacc accggtgtca ccgcgccagg
    36241 ttgcaaaaac aaaatcggca tccagttcag gtaaacgctc cgcgctcaca tccattcgcc
    36301 caccttcagg aatgctctca atcagcggcg gaaaacgaaa acccgcatcg cgtaataccc
    36361 gccccagcga atgatagcta tgcatcacat taatttttcc ctggtttgcc tgaatcaccg
    36421 atacagttat tttttggctg tccagcgtcg ccttcagcgc attgatttgt gcctgatagc
    36481 ggcgttcaag tatcgccagc tgcgactgcg ttcctgtaag ttctgccagc ttgcgataga
    36541 tttctggcgc gccccctttg agatgatcaa tgctcaccgt gggcgcaatt ttttcaagtt
    36601 gttcaatggg cgtattacgg gtcggttctg tgatgatcag gtcaggcttt gccgccacaa
    36661 tcgcttcaat atcaatatca gcagtaccaa taaaggcgat cgatgaatta tcaaagtcga
    36721 cacctgtcaa taacgcgcca gatcgaatga aatggctacc gtcaggccga gttcgcccgt
    36781 ggctggcgac aggaggtaca ccgagctcaa tcagaggaat agtgatatca agatcgtgta
    36841 aagagacaat tctctttgga tgagcaggaa cagtgacttt gcgccctaaa tcatcagtaa
    36901 atacctgaac aggttctgtt gcacgggaaa aaaatgacaa cagtatcagc agagaaaaaa
    36961 ataaacgcat ataactcccg ttaaaaactg ttcctgcgct gccagagcag tagcaaaaaa
    37021 aatggtccac caatcagtga gataatgatt ccggcaggca gttgcaaagg caaaaaagcc
    37081 aaacgaccaa tgctatccgc cagaataact aacaacgccc ccgataccgc gctccccagc
    37141 agtaatgtcg tctgcccgcc acgtagaata aaacgtgata tatggggtgc tatcaatccg
    37201 acaaaaccga tgttaccaac acacgaaacg cagacggctg tcaggattat gggtgcggcg
    37261 acacgtaaca acgccagacg agaactattc acccctaacc cggttgcaac ctgatgacca
    37321 agcagagcga tatccgcagt cctggcggtg aacagcaata aaagagcggc tggcaacatc
    37381 caacaggctg aaataccgac taacatccag ttagctgcat gcaggcttcc cgataaccac
    37441 atcaatgcag tttgtacatc tcgaacatct gcagtggtca taaatacacc gataccggcg
    37501 gcgaaaaacc aggaaacacc aataccaatc agaacaaatc gcgggcggga aatatcgcgc
    37561 gcacaaaaaa tcacgattaa agccaccaac aggccaccag caagaccagc cactggacgc
    37621 cagaacattc ccagcattgg aaactggaaa atcagccaca gcacagccac gctacatcct
    37681 tctttgacgc cgattaaccc aggatcggca aggccgttac gtgcaatgga ttgcattgcg
    37741 gcccctgcca tacctaacat ggccccgcat agcaatgcca tgatgactct cggcaagcga
    37801 atatcctgca caatataacg tgcgtctgtg cttacgtttt ctggagagaa caatgctcgt
    37861 cctacttccg atgccgggac ggagaaagag ccatgcatca gaccgaatat cagtattcca
    37921 accgcaaaca gaaacaaccc accaagcaac agcagatttt tagggcgtac cagtgtcgaa
    37981 aacttaccaa cacgtagtgg gcgtagcccg acgttcatta tccgactcat ttgaacatcc
    38041 tcgtcgccat aatgacaaaa acaggcgcac caactaacgc cgtcatgaca ccggtcgcta
    38101 actcataggg cgtgaaaagc gtccgggcaa taatatctgc cagtaataac aaaagcgcgc
    38161 cgacacaagc tgaaagaggg agcagaacgc gcagatccgc tgataccaga cgccggacaa
    38221 tctgcggtac tagcaggccg acaaaaccaa tggggccagc aatcgatacg gctgcgccac
    38281 agagcaacgc gatcgcaagc agggtgaatg ttcgcgtgcg gagtacagag acgcccagcc
    38341 cctgcgccat gcgatcgccc agtgccaaca tgttaaggga gggtgcaagg taaagggcta
    38401 acacaaatcc ccccagagaa aaccaggcgg acgtacccag cgtcgcccaa tcctgccccg
    38461 ctaaatcacc tgctagccat gtccgcatcg ccagcagtgt ttgttcgtca agaatcagga
    38521 ttgcagccgt tattgaggac acgaacgccg atagcgccac gccgcataaa gtaactttca
    38581 ttggcgttaa ccctgaacgc cctgcggagg aaagcagcaa tatcaacagg aacaataacg
    38641 cgccccccgt agatgccagc agtgggcgtc caaccggaaa ggccagtcca agcgcactgg
    38701 cggcaacgac agcaagagcg gctccggcat ttaagcctaa aatatgtggc tcccccaatg
    38761 ggttacgaat gacagcctgc aacaaagcac cagcaacacc aagagaagca ccggtaagca
    38821 atgctgccgc taaacgcggc aaacggagtc ttacaattac gttgtgatcg aagttttgcg
    38881 gatcaaaatg aagaaatgcc tgcaacaccg tttgtggtgc aataaatcgg gcaccaatcc
    38941 ctggatatac cacgcatccc accagcagta aggcgaggaa aattacagta accagccctg
    39001 tgcgcatcat actttttcta caccacgaaa cggcatgaag aacggctttc ccgtcagagg
    39061 atttattgat gcgtgaacat caacgtcaaa gacagctttt accagttcag gcgtgcaatg
    39121 ctccccctca ttcaacacac gcaccacttt cccctggcgt aaaaatatca acgtatcgcc
    39181 ataattgacg gcaaaattca gatcatgcaa caccacgacg actgtacgcc cgtgatgacg
    39241 cgttaaatca tgtaataact caagaatttc aacctgataa cgtaaatcaa gccaggttgt
    39301 gggttcatcc agcaggatat aaggcgtctt ttgcgccaac accatcgcaa tccagcaacg
    39361 ctggcgctgc ccaccagaga gtttttcaac aggtaaatgt gcaaactcct gcgtaccggt
    39421 taatttcagg gcctcttcca ccgcctgctc atcggcgtcg ctccattgac gtatgaaatt
    39481 ttgccaggga aaacgcccac gcgacaccag ttcataaacg gttaagccct ccggcaataa
    39541 tggcgactgc ggcaagatcc caagtctacg ggataacgct ttcgttggtt gctcgtgaat
    39601 cgctttgcca tcaagcaata tcgaaccgcc cataggttgc aagatacgtg caatggtgct
    39661 gagcaacgtc gactttccac agccgtttgc cccaaccaga acggtcattt tttctgtggg
    39721 aacggagaaa gaaacaccat caacaatgat cttcttgtga tacccggcag aaacattgtc
    39781 gaggatcagc ccctgccctt ttgcgttatg tgccacgtgt acgccaactt aatcaaagac
    39841 aaacaaaaat aaaactcatt ctcctttgag agtttgacgc cacaaaatgt agtagtcaag
    39901 cag
//
LOCUS       DAZFJL010000024        39036 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFJL010000024 DAZFJL010000000
VERSION     DAZFJL010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.024"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            100..657
                     /gene="hxlB"
                     /locus_tag="ACHJ8C_001289"
     CDS             100..657
                     /gene="hxlB"
                     /locus_tag="ACHJ8C_001289"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFO5551179.1"
                     /translation="MDAKLYITEIINELTFVKDSIREDDCTKLIDAIQQSQRVFCYGL
                     GRAGFSMKAFTMRLMHMGKEVYFLTETITPNFGPGDLFIVSSASGETAQLVALANKAR
                     QLGGAVAVLTTNRHATITEFVDVIVQINAPSKNQKDSVFRSAQPMASLYEQALLVIAD
                     ALVMKMAAESGAPESELFKRHANLE"
     gene            699..2207
                     /gene="ptsG"
                     /locus_tag="ACHJ8C_001290"
     CDS             699..2207
                     /gene="ptsG"
                     /locus_tag="ACHJ8C_001290"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-specific PTS transporter subunit IIBC"
                     /protein_id="HFO5551180.1"
                     /translation="MSKKITSLFNFKKLQKFSSALMLPIAILPAAGLILAIGISFNIE
                     LLANCGGLIFANLPLLFCVGIVIGLTGDGSSSLSAIISYMIMNATMGTQLGITAESVA
                     ASNGDYAMILGVPTLQTGVFGGLIVAIFVYLLYTRFHNIELPQFLGFFGGKRFVPIIT
                     AVAAIIVGYLLPWLWIPVQKGLASLSTLVTSGHNANFAAFIYAVGERSLIPFGLHHIW
                     NVPFYYNFGDYMTKSGQLVTGDIPVFFAQLRDGVPLTAGLFMTGRFPIMMFALPAAAL
                     AMYQEAKPERKALIKGLLLSGALTTFVTGITEPLEYAFLFAAPVLYIIHIFLYATSFV
                     LMNILNVHIGHPFAGGLIDFVLNGVMPNRTPWYLVFLAGAGYAAIYYTVFRVLIRKLN
                     LKTPGREDEEIAVATTLTREERPYQIIEAVGGFDNIEDVDACATRLRLALVDDKKVNE
                     KRLKELGAAGLVKLGDGGVQVIFGGKSQILRDEIKTVMSRPRPTEMTVACAN"
     gene            2258..4255
                     /gene="tkt"
                     /locus_tag="ACHJ8C_001291"
     CDS             2258..4255
                     /gene="tkt"
                     /locus_tag="ACHJ8C_001291"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO5551181.1"
                     /translation="MNSQLLANAIRMLSVDAIQKANSGHPGAPMGMADIAEVVWRRHL
                     RHNPKNPQWFNRDRYVQSNGHGSMLIYALLHLTGYDLSMDDIRDFRQLHSRTPGHPEY
                     GYTPGVETTTGPLGQGVANAVGMAIAEKALAAEFNKPGFNIVDHHTWLFLGDGCLMEG
                     ISHEACGLAGTLKLGNLIAIWDDNGISIDGHVEGWFAEDTAARFRAYGWHVIEGVDGH
                     DPEAVDAAVREAKSVTDKPSLLCCKTIIGFGSPNKANSHDCHGSALGADEVALVRERL
                     QWPYAPFEIPGEIYAEWDATEKGAQVQQEWDALFADYAKQWPELAAEFTRRMKGDLPA
                     GWAENMQKYVHDLQSHPAALATRQVSQKCLNHFADMLPELMGGSADLSPSNLTRHQKS
                     VDFTGENPAGNYISYGVREFGMSAIMNGLALHGGFIPYGGTFLMFMEYARNALRMAAL
                     MKIRSVFVYTHDTIGLGEDGPTHQPVEQLASLRLTPNMETWRGCDQVEVAVAWQQAIE
                     RKDGPTSLVLTRQPLAQQPRTAAQLAEIARGGYVLSDCDGQPEMILISAGSEIELVVS
                     AAKALTEEGRKVRVVSMPCTERFDNQDAAYKESVLPKAVRKRLAVEASIAGFWERYVG
                     LDGKVIGMTSFGESAPANVLFKHFGFTPENVLAQARELLNS"
     gene            complement(4285..5130)
                     /locus_tag="ACHJ8C_001292"
     CDS             complement(4285..5130)
                     /locus_tag="ACHJ8C_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO5551182.1"
                     /translation="MNNITVKSLIQSNYPTLHQAEKKVADYILSHAHEVVNYSVTELS
                     EKSHASEATIVRTCKKLGYQGYYHLKIALAKEVINPDNSYPDNTDFTDITSLATFLLK
                     KQAEDLIQSTQFFNAEVLESILKLLANCDTIFFFAAGNSNPLAVYGAYKFSQLGLKTV
                     VHVSPEMQINAAYSMGKRDLAIGISNSGSTNLMVDIFKVVKERGAKSVCITNYIKSPL
                     SKLSTHQLNTAVSDKIFFEAFDSTRVPAMGVIDMLVLLFMYKNKAHYEKYRTREEFLG
                     SRFKG"
     gene            complement(5330..6235)
                     /locus_tag="ACHJ8C_001293"
     CDS             complement(5330..6235)
                     /locus_tag="ACHJ8C_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5551183.1"
                     /translation="MFISDETLRVIHLVARHQSITTAAEQLNKVPSAISYTIKKAEES
                     LGVELFIRKGRYIELSPAGSYFIEHSKTILGDLEALKRNTVLIHDGIERELTIAVNNI
                     IPGDLLVAFIRDFERQFTSTTLTVDLEVYNGCWDALYSKHADLVYGAPHAVPSSEGII
                     SEPVGQMEWDFVVSPLHPLAAKRHPLENSELRHYPAVCIRDTSVNFPPMQAWLLEGQK
                     PIFVPDFATAIALIEQNVGIGYIPHHLALPLLNSGKLLKKPMREHKHATKLFLAARSD
                     GMGKACQWCIEYLRNPQLMTRFVFN"
     gene            complement(6313..7536)
                     /locus_tag="ACHJ8C_001294"
     CDS             complement(6313..7536)
                     /locus_tag="ACHJ8C_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO5551184.1"
                     /translation="MRKKEENLNTASGLRIAMILLGIAVTPVLLSSSSLGNQLSSGSL
                     ISVVLLGGVILTLLSAITISVGEKARLPTYGIVKYSFGEKGAIAINILMAISLFGWIA
                     VTANMFGHSVHDLLAQHGLEVPLALLVAAGCVIFVASTAFGFAVLGKIAQVAVPVIAL
                     VLCYILYVATHTEVAVPAAIVEMNTGVAVSTVVGTIIVLVATLPDFGSFVHNRKHALI
                     AAGVTFLVAYPLLYWAGATPSAISGQGSLLGAMAVFGAVLPAALLLIFACVTGNAGNM
                     FQGTLVVSTLLTRFPKWQITVALGILSAIVGSMDIMAWFIPFLLFLGIATPPVAGIYI
                     ADFFLYRRNGYQESVLAQESQIKVLTFAAWIIGAAVGFMTVKGLFTLTTIPSVDSILV
                     ACIAYAILSRASQHR"
     gene            complement(7562..7951)
                     /locus_tag="ACHJ8C_001295"
     CDS             complement(7562..7951)
                     /locus_tag="ACHJ8C_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HFO5551185.1"
                     /translation="MTTTLLAVNGTLMRGLELNPNMQKAGGIFVREDRTDAHYRLWSI
                     NDRHPGMIRVNEGGTHVDVEIWQLPLASFAALLMSEPAGLAIGKIKLADGSEVLGVLA
                     ENWLTEGQREITELGSWRKYTGHFHTV"
     gene            complement(7968..8924)
                     /gene="arcC"
                     /locus_tag="ACHJ8C_001296"
     CDS             complement(7968..8924)
                     /gene="arcC"
                     /locus_tag="ACHJ8C_001296"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO5551186.1"
                     /translation="MVKPLAVVAVGGNALIQDEQRNSIPDQYVAVMESVQHIVDMVEA
                     GWDLVLTHGNGPQVGFILRRSELASNEVSPVPLDYAVGDTQGAIGYMFQKALHNELAR
                     RGINKPVIALVTQTRVSPHDDAFASPSKPIGAFLDEATAQQRQQQLGWTLMEDAGRGW
                     RRTVPSPAPLEIIEHDTIAHLVRQGYLVIACGGGGIPVVRDGQQLKGVEAVIDKDLAS
                     ALLASQLGADLLVIPTGVEKVAINFGTPQQQWLDAISVAEAQTLLREGQFGVGSMQPK
                     VEAIVDFINASQQQGKQASGLITSPQTIKAALAHQSGTWITL"
     gene            complement(8917..10341)
                     /locus_tag="ACHJ8C_001297"
     CDS             complement(8917..10341)
                     /locus_tag="ACHJ8C_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO5551187.1"
                     /translation="MNTLFNQPLKVVNAGLHSFADNIQHAGGSAIALNWQPPAQGDID
                     AGLDLASLLRHPLVENANQIAMTRYLEAQPVLVDVMLAKEAIPAMAEQKRILHSGPPI
                     AWEEMCGPVKGAIIGAMLYEGWATSQQDAENQINAGEIDLAPCHHYHAVGPMAGIISP
                     SMPLWVVENKTNGHRTFSNFNEGLGKVLRFGANNDEVLNRLAWMRDELTPAMKAAIAQ
                     HGELELKPLMAQALHMGDEVHNRNAAATGLLIKRLLPALLACSLPQEHIQRVVAFITG
                     NDHFFLNLSMAACKAMMDAAANVPFSSMVTVMARNGVNFGIRLSGTGDRWFQAPANAV
                     EGLFFPGFGVDDAAADLGDSAITETAGVGGFAMASSPAIVKFVGGTPADATNNSRRMQ
                     AITLGGNPAFTLPALNFAPTAAGIDARKVTDRGILPVINTGIAHKQAGVGQIGAGITT
                     APMACFVAAVRALAEIVAKENHHG"
     gene            complement(10338..11897)
                     /gene="fdrA"
                     /locus_tag="ACHJ8C_001298"
     CDS             complement(10338..11897)
                     /gene="fdrA"
                     /locus_tag="ACHJ8C_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO5551188.1"
                     /translation="MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNL
                     EQLRDAGLGNEINASPNDLVIAVMGEEDICNEALALAQQRLTSKPDDETDSGIKTPEK
                     VSLEMALEAEPEANLALISVPGDYAAAEAMKALNLGMNVMMFSDNVSIGQEKSIKTLA
                     RERQRIVMGPDCGTAIVNGIPLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGAGIS
                     QALGTGGHDLSEEIGGISMLFALDALAQDDETRVIVLISKPPSPIVARTILERAEACG
                     KPVVVNFLGANPHDLARPNITAATTLASAANIAVALLNDQPLPAVETEISCADLTMLQ
                     NACQRLPAHRQAIRGVFAGGTFCYEAQLICQQKGFSAASNTPVAGNRALANIWQSEDH
                     TLIDMGDDDFTRGKPHPMIDPTLRNQRLLNELNDSHTAVVLFDLVLGYGASTTPASEL
                     LDQLSHIDMNNAPLLIAHVCGTEADPQIRSQQISALQNAGIIIASSNAQAALWASAVA
                     QTQLQKKGLNA"
     gene            complement(11992..12852)
                     /locus_tag="ACHJ8C_001299"
     CDS             complement(11992..12852)
                     /locus_tag="ACHJ8C_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO5551189.1"
                     /translation="MVQVTLCASSLGYLFPQNETQDLRLHSAFKHAVNLYSDAGTFIT
                     LLCAQTYLNLPDAARVWLPECWDWRREIAHSDPIQLTQGLLRTPRFCVALENATLWQS
                     PFVGRMLTLEAFPLVFQHYPTMASQRLLFCLEHNVQSTLHLPDSLTHQGLAIMEHPDA
                     LERQVPQLIGFGKGLTPDGDDYLLGYLAALWLWQLPAPLADHQYRLQQAIDQHAHNTT
                     DISRHYLERALQGHFSEPICQLLAQLVGSASAMTIASCAEQVMQFGATSGVDCLAGML
                     HGFRTLNTMN"
     gene            complement(12858..13526)
                     /locus_tag="ACHJ8C_001300"
     CDS             complement(12858..13526)
                     /locus_tag="ACHJ8C_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFO5551190.1"
                     /translation="MTQSIFQAQPFELPFDPRTTALVMIDMQRDFVEAGGFGEALGND
                     VSLVRTAIAPCTEVLAAARQKGIMVIHTREGHRADLSDCPPAKLTRGGKTFIGEPGPM
                     GRILVRGEAGHDIIPELYPVVGEPVIDKPGKGAFYQTDLHLILQNHGIKTLIVCGVTT
                     EVCVTTTVREANDRGYECIIPEDCVGSYFPEFQKYALEMIKAQGAIFGWVTDSKAIIA
                     GLEG"
     gene            complement(13783..14592)
                     /locus_tag="ACHJ8C_001301"
     CDS             complement(13783..14592)
                     /locus_tag="ACHJ8C_001301"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO5551191.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFTQYGVKK"
     gene            14854..15618
                     /gene="udp"
                     /locus_tag="ACHJ8C_001302"
     CDS             14854..15618
                     /gene="udp"
                     /locus_tag="ACHJ8C_001302"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO5551192.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLLK"
     gene            complement(15653..17143)
                     /locus_tag="ACHJ8C_001303"
     CDS             complement(15653..17143)
                     /locus_tag="ACHJ8C_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HFO5551193.1"
                     /translation="MFESELLTQGFSTLLNNPQALLFATFGVMLGIVIGALPGLTATM
                     GVAILLPFTYGMEPVSGLLMICGVFFGGVYGGSITAILLKIPGTPAAAATAIDGYELT
                     KQGKAGLALSAATFSSFSGGTLSIIVLMFLSPVLASWALKFSASESFALATFGLSIIA
                     SISGESLIKGLIAGVGGLLIATIGLDPMGGFPRFTGGFVELMNVPFIPVMIGLFAASE
                     AFRSMEQNQQIRRGAKVAIGSLLLPWQTLRRIALTILRSSGLGVFIGMIPGAGADIAA
                     FVAYNETRRFSKTPENFGKGEIKAVASCEAGANGCTGGALLPMLTLGIPGDAVTAIML
                     GALTLQGMQPGPLMFTDHGDMVYTLFVGMIFCYFMLLVLGLLSLKVIGNVVKIPGNIL
                     TPMILALCVVGTYALNNSLFDVGIMLIAGVVGYFMQKGGYPASPVVLALIMGPMAESN
                     FRRALSLSGGSLDFLYTRPITLALLTLAAFTLLTPIIRKIMRLRRQ"
     gene            complement(17156..17662)
                     /locus_tag="ACHJ8C_001304"
     CDS             complement(17156..17662)
                     /locus_tag="ACHJ8C_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HFO5551194.1"
                     /translation="MNTKQSVAQLAVPHRKRLSSTMVVALLLCVVAGAVMINAADFPA
                     TAIETDPGASAFPTFYACALIVLAVLLVIRDLLQAKPASCANAQEKPAFRKTATGIAA
                     TAFYIVAMSYCGYLITTPVFLIVIMTLMGYRRWVLTPGIALLLTAILWLLFVEALQVP
                     LPVGTFFE"
     gene            complement(17679..18653)
                     /locus_tag="ACHJ8C_001305"
     CDS             complement(17679..18653)
                     /locus_tag="ACHJ8C_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HFO5551195.1"
                     /translation="MIKHLTLPKKMAAMVLLAGAALSIAPVQAASWPTKQIELVVPYA
                     AGGGTDLVARAFADAAKNHLPVSIGVINKPGGGGAIGLSEIAAARPNGYKIGLGTVEL
                     TTLPSLGMVRFKTSDFKPIARLNADPAAITVRADAPWNSYEEFMTYAKANPGKVRIGN
                     SGTGAIWHLAAAALEDKTGAKFSHVPYDGAAPAITGLLGGHIEAVSVSPGEVINHVNG
                     GKLKTLVVMADERMKTMPDVPTLKEKGVDLSIGTWRGLIVSQKTPQDVVDVLAKAAKE
                     TAEEPAFQDALQKLNLNYAWLDAASFQTQISEQEKYFDELLTRLGLKK"
     gene            complement(18678..19307)
                     /locus_tag="ACHJ8C_001306"
     CDS             complement(18678..19307)
                     /locus_tag="ACHJ8C_001306"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO5551196.1"
                     /translation="MPFNTLLQKTGLVAILRGVKPDEIVAIGEKLYAAGFRLIEIPMN
                     SPEALQSISLLRDALPKDCLVGAGTVLSVEQVVAVKEAGGQIIVMPHCDTAVIRRARA
                     LGMYCAPGVATPTEAFAAIEHGANAIKLFPAEQITPEVTKAWRAVIPQSVPMLPVGGI
                     TPETMARYLSHGANGFGLGSALYHPGMTPEQVYENAVLFMNAWNNLNSN"
     gene            complement(19297..20301)
                     /locus_tag="ACHJ8C_001307"
     CDS             complement(19297..20301)
                     /locus_tag="ACHJ8C_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO5551197.1"
                     /translation="MPGAPSFIALDWGTSSLRAWRFGESPNPQEKREFPWGIMKLPSQ
                     AATREDAFHDTFLRVCGDWLAQTPCPVLACGMLGSAQGWQSAAYLPCPVTLEGLAKQL
                     TPVIHQQQTMLHIIPGVIKEGEMPEVMRGEETQIFGAISMEPALQNAIHQGMPVLIGL
                     PGTHAKWAVVENNTITDFRTFMTGELFDVLSRHSILGATMHPGDEPHWDAFTHGLTAA
                     QEHHQTGLLSTLFSTRSRLLTSNLTSSSQGDYLSGLLIGHELCGLASSLLRDLPATTP
                     IALIGSANLNSRYSQAFSHVFPDRQIHAIPNATEQGLWRIAHAAGLLSTNARECTHAI
                     "
     gene            complement(20325..21092)
                     /locus_tag="ACHJ8C_001308"
     CDS             complement(20325..21092)
                     /locus_tag="ACHJ8C_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO5551198.1"
                     /translation="MAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRST
                     THRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQPVLQRLARYTLD
                     TVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLF
                     KTCGDETKLGAFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVAST
                     TTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK"
     gene            21309..22736
                     /gene="rmuC"
                     /locus_tag="ACHJ8C_001309"
     CDS             21309..22736
                     /gene="rmuC"
                     /locus_tag="ACHJ8C_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO5551199.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYSR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            22831..23586
                     /gene="ubiE"
                     /locus_tag="ACHJ8C_001310"
     CDS             22831..23586
                     /gene="ubiE"
                     /locus_tag="ACHJ8C_001310"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO5551200.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKTMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            23600..24205
                     /gene="ubiJ"
                     /locus_tag="ACHJ8C_001311"
     CDS             23600..24205
                     /gene="ubiJ"
                     /locus_tag="ACHJ8C_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO5551201.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            24202..25842
                     /gene="ubiB"
                     /locus_tag="ACHJ8C_001312"
     CDS             24202..25842
                     /gene="ubiB"
                     /locus_tag="ACHJ8C_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO5551202.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            25921..26190
                     /gene="tatA"
                     /locus_tag="ACHJ8C_001313"
     CDS             25921..26190
                     /gene="tatA"
                     /locus_tag="ACHJ8C_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO5551203.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            26194..26709
                     /gene="tatB"
                     /locus_tag="ACHJ8C_001314"
     CDS             26194..26709
                     /gene="tatB"
                     /locus_tag="ACHJ8C_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO5551204.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNETAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            26712..27488
                     /gene="tatC"
                     /locus_tag="ACHJ8C_001315"
     CDS             26712..27488
                     /gene="tatC"
                     /locus_tag="ACHJ8C_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO5551205.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIISVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            27530..28312
                     /gene="tatD"
                     /locus_tag="ACHJ8C_001316"
     CDS             27530..28312
                     /gene="tatD"
                     /locus_tag="ACHJ8C_001316"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO5551206.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAVTEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQELAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPRILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(28309..28797)
                     /gene="rfaH"
                     /locus_tag="ACHJ8C_001317"
     CDS             complement(28309..28797)
                     /gene="rfaH"
                     /locus_tag="ACHJ8C_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO5551207.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPSTPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            28964..30457
                     /gene="ubiD"
                     /locus_tag="ACHJ8C_001318"
     CDS             28964..30457
                     /gene="ubiD"
                     /locus_tag="ACHJ8C_001318"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO5551208.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVTCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            30503..31204
                     /gene="fre"
                     /locus_tag="ACHJ8C_001319"
     CDS             30503..31204
                     /gene="fre"
                     /locus_tag="ACHJ8C_001319"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO5551209.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(31476..32639)
                     /gene="fadA"
                     /locus_tag="ACHJ8C_001320"
     CDS             complement(31476..32639)
                     /gene="fadA"
                     /locus_tag="ACHJ8C_001320"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO5551210.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(32649..34838)
                     /gene="fadB"
                     /locus_tag="ACHJ8C_001321"
     CDS             complement(32649..34838)
                     /gene="fadB"
                     /locus_tag="ACHJ8C_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO5551211.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDTPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVIMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFNANRFGQKNGLGFWRYKEDSKGKPKKGEDAAVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEQYYPPVEPARPVGDLKTA"
     gene            35028..36359
                     /gene="pepQ"
                     /locus_tag="ACHJ8C_001322"
     CDS             35028..36359
                     /gene="pepQ"
                     /locus_tag="ACHJ8C_001322"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO5551212.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPNSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            36359..36973
                     /locus_tag="ACHJ8C_001323"
     CDS             36359..36973
                     /locus_tag="ACHJ8C_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO5551213.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            37012..38463
                     /gene="trkH"
                     /locus_tag="ACHJ8C_001324"
     CDS             37012..38463
                     /gene="trkH"
                     /locus_tag="ACHJ8C_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO5551214.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFSLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            38475..39020
                     /gene="hemG"
                     /locus_tag="ACHJ8C_001325"
     CDS             38475..39020
                     /gene="hemG"
                     /locus_tag="ACHJ8C_001325"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO5551215.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         9245 a         9846 c        10413 g         9532 t
ORIGIN      
        1 ctttcttgtg actagcttca catttcatac tgatgcattg gaatgaaata aaatttcatg
       61 agattttgtg tttgagaaaa ttatttcatg aggtgtgaaa tggacgcaaa gctgtacatc
      121 actgagatca tcaatgaatt aaccttcgtt aaggactcta tccgtgagga tgactgcacc
      181 aaattgatcg acgctattca gcaaagccag cgcgttttct gctatggcct ggggcgtgcc
      241 ggcttttcca tgaaagcctt caccatgcgc ctgatgcata tgggcaaaga ggtttacttc
      301 ctgacggaaa ccattacgcc gaactttggg cctggcgatc tgtttatcgt ttcttccgct
      361 tccggtgaga cagcacaact ggtggcactg gcgaataaag cacgtcagct tggcggcgct
      421 gtggcggtgt taaccacgaa tcgacacgcc actatcaccg agtttgtcga tgtcatcgtg
      481 caaattaatg ccccttccaa aaatcaaaaa gattctgttt tccgctcggc acagccgatg
      541 gcgtcgttgt atgagcaagc tttgctggtg attgccgatg cattagtgat gaagatggct
      601 gctgaaagcg gtgcgccgga aagtgaactg tttaagcgcc acgccaatct ggaatagtcg
      661 tcgccgttat ttgctcgata ccctacagga gaataaaaat gagcaaaaaa ataacatcct
      721 tgtttaactt caagaagttg cagaaatttt cttctgctct gatgttacca atcgccattc
      781 ttccggcggc gggtttgatt ttagccatcg ggatttcatt taacattgag ctgctggcga
      841 attgcggcgg cctgattttc gccaaccttc ctttgctgtt ttgtgtcggt attgtgattg
      901 gcctgacagg tgatggttct tcttcacttt cagcgattat cagctacatg atcatgaatg
      961 ccacgatggg tacgcaactt ggcattaccg cggagtccgt tgcagccagt aacggtgact
     1021 atgcgatgat cctcggggta ccgacgttac aaacgggagt gtttggcggt ttgattgtcg
     1081 ccatctttgt ttatctgctc tatacccgct tccacaatat tgaattgcct cagtttcttg
     1141 gtttcttcgg tggaaaacgc tttgtaccga ttattacggc ggtggcggca attattgtcg
     1201 gctatttatt gccgtggctg tggataccgg tacaaaaagg tctggcgtcg ctttcaacgc
     1261 tggtgaccag tgggcataac gcgaacttcg cggcctttat ttacgccgtt ggcgaacgtt
     1321 cgctgattcc ttttggcctg catcatatct ggaacgtgcc gttttattac aactttggcg
     1381 actacatgac caaatccggg caactggtga cgggcgatat tccggtgttc tttgcccaat
     1441 tgcgcgacgg tgttccgttg acggcggggc tgtttatgac aggtcgtttc ccaatcatga
     1501 tgtttgcgtt acctgccgcc gcgttggcga tgtatcagga agcaaaaccg gaacgtaagg
     1561 cgctgattaa aggcctttta ctctccggtg cgctgaccac atttgttacc gggattacgg
     1621 agccgctgga atatgcattt ttgtttgcag ctccggttct ctacatcatt cacatcttct
     1681 tatatgccac ttcatttgtg ctgatgaaca tcctgaatgt gcacattggt catccttttg
     1741 ctggcggtct tattgatttc gtactgaatg gcgttatgcc aaaccgcacg ccgtggtatc
     1801 tggtgttcct ggcaggcgcg ggctatgcag cgatttacta caccgtattt cgggtactga
     1861 tccgcaagtt gaatctgaaa acaccggggc gtgaggatga agagattgcc gttgcaacca
     1921 cattaacgcg cgaagaacgt ccataccaaa tcatcgaagc agtaggtggt ttcgacaata
     1981 tcgaagatgt ggatgcctgc gcaacacgcc tgaggctggc gctggtagac gataagaaag
     2041 tgaatgaaaa acggctgaag gagttggggg ctgcgggttt agtgaaactg ggtgatggcg
     2101 gtgtacaggt gatatttggc ggcaaatcgc aaatcctgcg cgacgaaatc aaaaccgtaa
     2161 tgagcagacc gcgcccgaca gaaatgacag tggcctgtgc taactaattc gagattagcg
     2221 ggctggtttc agcccgccag acagagagag ctgaagtatg aattcacaat tactggctaa
     2281 tgccatccga atgttaagtg ttgatgcgat ccagaaagcg aactccggtc atccgggcgc
     2341 gccgatgggg atggcggata ttgccgaagt ggtctggcgt cgtcacctgc gtcataaccc
     2401 aaaaaatccg cagtggttta accgcgaccg ctatgttcag tcgaacggtc acggctccat
     2461 gttgatttac gctttgctgc atcttacagg ctacgacctg tcgatggatg atattcgcga
     2521 ctttcgtcag ctccactccc gcacgccggg tcatccagaa tatggttata cgccgggtgt
     2581 tgaaaccacc accggcccgt tggggcaggg cgtagctaat gcggtgggga tggcgattgc
     2641 ggagaaggcg ctggcggcag aatttaacaa accgggcttt aatattgttg atcatcacac
     2701 atggctattt ttgggtgatg gctgcctgat ggaagggatc tcccacgagg cctgtggtct
     2761 tgcaggaacg ctgaagctcg gcaatctgat tgctatctgg gacgataacg gtatttcgat
     2821 cgatggtcac gtagaaggct ggttcgctga agatacggct gcccgttttc gtgcctacgg
     2881 ctggcatgtc attgaaggcg ttgatggtca cgatccagaa gcggtagatg ctgcggtgcg
     2941 tgaagcgaaa agcgtcacgg ataagcccag tctgctgtgc tgcaaaacga ttatcggttt
     3001 tggttcgcca aataaagcca acagccacga ttgccacggc tcggcgctgg gtgcagatga
     3061 agtggcgctg gtgcgtgaac gtctgcaatg gccgtatgcc cctttcgaaa ttcctggtga
     3121 aatttatgcc gaatgggatg cgaccgagaa aggcgcgcag gttcaacagg aatgggatgc
     3181 actgtttgcc gactacgcga agcagtggcc ggaactggcg gcggagttta ctcgtcgcat
     3241 gaagggtgat ttgcctgctg gctgggccga gaatatgcag aaatatgtgc acgatctgca
     3301 atcccatccg gcggcgctgg caacccgtca ggtcagccag aaatgcctca atcacttcgc
     3361 ggatatgctg ccggaactga tgggcggctc ggcggacttg tcgccctcta acctgactcg
     3421 ccatcagaaa tcggttgatt tcaccgggga aaatccggcg gggaattaca tctcctatgg
     3481 cgtgcgtgaa tttggtatgt cggccatcat gaacggtctt gcgctgcatg gtgggtttat
     3541 cccttacggc ggtactttcc tgatgtttat ggaatatgcg cgtaatgcac tgcgcatggc
     3601 ggcgctgatg aagatccgct cggtgtttgt ttatacccac gataccatcg gcctgggcga
     3661 agacggcccg acacatcagc ccgttgagca actggcgtcg ctgcgtctga cgccgaatat
     3721 ggaaacctgg cgcggttgcg atcaggtgga agtggcggta gcctggcaac aggcgattga
     3781 gcgcaaagat ggcccaacgt cgttggtatt aacgcgccaa cctctggcgc aacagccgcg
     3841 caccgctgca cagttggcag agattgcgcg tggtggttat gtactgagtg attgtgacgg
     3901 tcagcctgag atgatcctca tttctgcggg ttcagagatt gagttagtgg tcagcgccgc
     3961 aaaagcgtta acggaagaag ggcgtaaagt tcgcgtggtt tctatgccgt gtaccgagcg
     4021 tttcgataat caggatgcgg cgtacaaaga atcggtattg ccaaaagctg tacgcaaacg
     4081 tctggcggta gaagccagca tcgccggatt ctgggaacgc tacgtgggcc tggacggtaa
     4141 agtgatcggc atgacgagct ttggtgagtc tgctccggcg aatgtgttgt tcaaacactt
     4201 tggttttacg ccggaaaatg tcctggcgca ggcgcgtgag ctactgaaca gctaaacctg
     4261 cacaaggggt gaaaggatga ccttttaccc cttgaaacgg gagccaagaa actcttcacg
     4321 ggtgcggtat ttctcgtaat gcgccttgtt tttatacata aacaacagca ccagcatatc
     4381 aataacgccc attgccggaa cgcgagtgga gtcgaaggct tcaaagaaga ttttatcgct
     4441 gaccgcggta ttgagttgat gggtggagag ttttgataac ggagatttaa tatagttagt
     4501 gatacaaacg gatttggcac cgcgctcttt caccacttta aagatatcga ccattaagtt
     4561 ggtgctgcca gagttagaaa taccaatcgc caggtcgcgt ttccccatag aatatgccgc
     4621 attaatttgc atttccgggc tgacgtgaac aacggttttt aatccgagtt ggctgaattt
     4681 ataagcacca taaacggcca gagggtttga atttccggca gcaaaaaaga aaatagtatc
     4741 gcaattagca agtagtttga gaatggactc aagcacttcg gcattaaaaa attgcgttga
     4801 ttgtattaaa tcttcggcct gtttttttaa taaaaatgtg gccagcgacg ttatatcggt
     4861 aaaatcggta ttatcaggat agctattgtc aggattaatg acttcttttg ctaatgcaat
     4921 ttttaaatgg taataaccct gataacctaa tttcttacag gtgcgtacga tagtcgcttc
     4981 actggcatgg cttttttctg atagttcagt gacactatag ttcacgactt catgtgcgtg
     5041 gctaagaata tagtcagcga cctttttttc cgcctgatgc agtgtagggt aattgctttg
     5101 aatcagactt tttacggtta tgttattcat aattagtaaa tttcattctg acagaaagtg
     5161 ctgattataa catagttttc aggcgtggat aggtgtgaaa aaatgaatgt gtgcagtgaa
     5221 cgtaatgatt aacagaccgg ttaattttca tgaattacgt caactgcaca aaaataattt
     5281 aaacgggtgc tctaattaaa gcaagactta tatttttatc attgtattgt caattaaata
     5341 caaatcgtgt cattaattgt gggtttctta aatattctat gcaccactga caggctttgc
     5401 ccataccatc ggaacgagcg gcaagaaata acttggtggc gtgtttatgt tcgcgcattg
     5461 gctttttaag caatttaccg ctgttcaata acggcaatgc cagatgatgt ggaatataac
     5521 caataccgac gttttgttct attaaggcga ttgccgtagc gaagtcagga acaaaaatgg
     5581 gtttttgtcc ttccaacaac caggcttgca tcggggggaa gttaacggag gtatcgcgga
     5641 tacaaaccgc tggataatga cgcaattcac tgttttctaa tggatgccgc ttcgctgcca
     5701 gtgggtggag tgggctgacg acaaaatccc attccatttg tccgaccggt tcgctaatga
     5761 tgccttcact gcttggtact gcatgaggag caccgtagac cagatcagca tgcttactgt
     5821 acagcgcatc ccagcagccg ttgtatacct caagatctac cgtcagtgtt gttgatgtga
     5881 attgccgttc aaaatctcga ataaatgcca ccagcaaatc accaggaata atattgttta
     5941 ccgcaattgt cagttcacgc tcaataccat cgtgaatcag cactgtgttg cgctttaatg
     6001 cttccaggtc gccaagaata gttttgctat gttcgatgaa gtaacttccc gctggcgata
     6061 attcaatata gcgtccctta cgaatgaaca gttcaacgcc caggctctct tccgctttct
     6121 tgatggtgta actgatggcg gaagggactt tatttaactg ctcggctgcg gtggtaatgc
     6181 tctggtgtct ggcgaccaga tgaataacac gaagcgtttc gtctgagatg aacataaggc
     6241 tccctggtca gtggtaaagt ggctgaatct ctccagattc agccgtaatc attgcctgag
     6301 tatacccagg aattagcggt gttgacttgc ccgactgaga atcgcatacg cgatacatgc
     6361 caccagaatc gagtctaccg aagggatcgt cgtcagggtg aataagcctt ttacggtcat
     6421 aaagccaacc gctgcgccta tgatccatgc tgcgaatgtc agcactttaa tctgtgactc
     6481 ctgggctaac actgactctt gatagccatt acggcgataa aggaaaaagt cagcgatata
     6541 aattccggca acgggtggcg tggcgatacc caggaacagc agaaacggaa taaaccacgc
     6601 cataatatcc atactgccta cgatggcgga aaggataccc agcgccacgg taatctgcca
     6661 tttgggaaag cgggtaagca gtgtggaaac caccagcgtg ccctggaaca tattgcccgc
     6721 gttaccggtg acgcaggcga aaatcaacaa cagcgccgca ggcagaaccg caccgaatac
     6781 cgccatcgca cccagtaaag atccctgacc actaatggcg ctcggcgttg cacccgccca
     6841 gtagagcaga gggtaggcaa ccagaaacgt cacgcctgcg gcaatcagcg catgtttgcg
     6901 gttatgcaca aaactaccga aatcaggcag tgtggcaacc agcacaataa tggtgccaac
     6961 aacggtggaa acggcgacac ctgtattcat ctccacaatc gccgctggta ctgccacttc
     7021 ggtatgggtt gccacataga ggatgtaaca cagcaccagc gcgataaccg gcacggcaac
     7081 ctgggcaatt tttcccagaa cggcaaagcc aaatgccgta gaggcgacaa aaatgacaca
     7141 gccagccgct actaacagtg ccagtggaac ttccagtcca tgttgagcca gtaagtcatg
     7201 taccgaatga ccaaacatat tggcggtaac ggcaatccag ccgaacagac ttatcgccat
     7261 caaaatgtta atggcgatgg cccctttttc gccaaacgaa tatttcacaa tgccatacgt
     7321 tggcaggcgg gctttttctc ccacgctaat ggtgatggct gaaagtaagg tcagaatgac
     7381 gccgcctaac aatacgacgc taattaaact gccgctggaa agttgattgc cgaggcttga
     7441 ggatgacaac agcacaggtg tgacggcaat acccagcaaa atcatggcaa tacgcaatcc
     7501 tgatgccgta ttcagattct cttctttttt acgcatagta tgttcccttt ataaatgcgc
     7561 gttacacagt atgaaaatgc ccggtgtatt tgcgccagct acccagttct gtaatttccc
     7621 gttggccttc ggtcagccag ttttctgcca gtacgcccag cacttcgcta ccatcggcaa
     7681 gtttgatttt gccaattgcc agcccagcgg gttcgctcat taacagagcg gcaaaactgg
     7741 ccagcggtaa ttgccaaatt tctacgtcga cgtgtgttcc tccttcattc acgcgaatca
     7801 ttcccggatg gcgatcatta atactccaca accggtaatg ggcgtcagtt cggtcttcac
     7861 ggacaaaaat accgcccgct ttttgcatat ttgggttgag ttccaggccg cgcatcagcg
     7921 ttccgttaac ggctaaaagc gttgtagtca ttgtgttctc cagagaatta aagggttatc
     7981 catgtaccgc tctgatgcgc cagggctgct tttatggttt gcggtgaagt aatcaggccg
     8041 ctggcctgtt tgccttgttg ctggctggca ttgatgaaat caacaatggc ttccactttg
     8101 ggttgcatac tgccgacacc aaactgacct tcccgcaaca gcgtttgcgc ttcggcaacg
     8161 ctgatagcgt cgagccactg ttgttgtggt gtaccaaagt taatcgccac tttttctaca
     8221 ccggtgggga tcaccagcag atctgcgcct aactgactgg cgagcagcgc ggaggccaga
     8281 tctttatcga tcacggcttc cacacctttc agttgttgcc cgtctcgcac caccggaata
     8341 ccgccgccgc cgcaggcaat aaccagatat ccctggcgca ccaggtgagc gatggtgtcg
     8401 tgctcaataa tttccagtgg tgcaggagag ggaactgtac gccgccaacc acgcccggcg
     8461 tcctccatca gcgtccagcc gagttgttgt tggcgttgtt gggccgttgc ttcatcgaga
     8521 aacgcgccaa ttggtttgct ggggctggcg aaagcatcgt catgtgggct gactcgcgtt
     8581 tgtgtcacca gggcaattac cggtttgttt atgccacggc gagccaattc gttatgcagc
     8641 gctttctgga acatgtagcc aattgcacct tgtgtatcac ccacggcgta atcaagtgga
     8701 accggagaaa cttcgttact ggcgagttca gagcggcgca gaataaagcc cacctgcggg
     8761 ccattaccgt gggttagcac caggtcccat ccagcttcaa ccatatcaac gatatgctgc
     8821 acgctttcca tcactgcaac atattgatcg ggaatactat tgcgttgctc gtcctgaatg
     8881 agcgcattgc cgccaaccgc gacgacagcc agtggcttaa ccatgatggt tctcctttgc
     8941 gacgatctcc gccagtgcac gtacagcggc gacaaaacag gccatcggcg cagtggtgat
     9001 cccggcacca atttgcccga cccccgcttg tttgtgggcg atacccgtat tgatgaccgg
     9061 taaaatgccg cgatcggtca ctttacgcgc atcaatccct gctgcggtgg gggcaaaatt
     9121 gagggctgga agtgtaaatg cggggttacc gccgagggtg attgcttgca tccgacggct
     9181 gttattggtg gcatcggcag gggtgcctcc cacaaatttg acgatcgccg gagacgacgc
     9241 catcgcgaag ccacccacac ctgcggtttc agtaattgcg ctatcaccca ggtcagcagc
     9301 agcgtcgtca acgccaaatc cggggaagaa cagaccttca acggcattgg caggagcctg
     9361 gaaccagcga tcgccagtac cggaaagccg aataccaaaa ttcaccccgt tacgcgccat
     9421 caccgtaacc attgaactga acggtacatt ggcggcggca tccatcattg ctttacaggc
     9481 ggccatcgac agattgagga agaagtgatc gttaccggtg ataaatgcca cgacgcgctg
     9541 gatatgttcc tgtggaagag agcaggccag caatgccggg agcaggcgct taatcagcaa
     9601 accggtagcg gcagcattgc ggttatgaac ttcatccccc atatgcagcg cctgcgccat
     9661 taatggcttg agttccagct cgccgtgttg tgcgattgct gctttcattg ccggcgtcag
     9721 ttcatcgcgc atccaggcca gacgattgag aacttcatcg ttattggcgc caaaacggag
     9781 aaccttaccc agaccttcat tgaaattgct aaaagtacgg tggccgttgg ttttgttttc
     9841 taccacccat aacggcatcg acgggctgat gatgcctgcc attgggccaa ccgcgtgata
     9901 atgatgacaa ggggcaaggt cgatttcgcc tgcgtttatt tggttttcag cgtcttgctg
     9961 gctggtggcc caaccttcgt acagcatggc accaataatc gcccctttta ccggaccaca
    10021 catctcttcc caggcaatcg gcgggccaga gtgcaaaata cgtttttgtt cagccattgc
    10081 tggaatggcc tctttagcga gcatgacatc caccagcacg ggttgtgctt cgagataacg
    10141 tgtcatggcg atttggttgg cattttctac caggggatga cgtagcaacg atgccagatc
    10201 caatccagcg tcaatatcgc cttgtgctgg tggctgccag ttgagtgcaa ttgcgcttcc
    10261 cccggcatgt tgaatattgt cagcaaaact gtgtagtccc gcgttcacaa ccttcaaagg
    10321 ctgattaaat aaggtgttca tgcgtttagc ccctttttct gcaactgagt ctgcgcaacg
    10381 gcgctggccc atagcgccgc ctgtgcattg ctgctggcaa tgatgatgcc cgcattttgt
    10441 agtgcgctta tctgctgact gcgtatttgc ggatcagctt ccgttccgca aacgtgcgca
    10501 atcagcaacg gagcgttatt catgtcgatg tgtgaaagtt ggtccagtaa ttcgctggca
    10561 ggcgtcgtgg aagcgccata accaagtacc aggtcgaata acacgacggc agtgtgtgag
    10621 tcgttgagtt cgttgagcag acgttgattg cgcagagtcg ggtcgatcat tggatgcggt
    10681 ttgccacggg tgaaatcatc gtcgcccata tcgatcaacg tgtgatcttc gctttgccag
    10741 atatttgcca gagcacgatt acccgcaact ggcgtattgg aagctgcgct gaatcccttt
    10801 tgttggcaga taagttgcgc ttcgtagcag aaggttcctc cggcgaaaac accgcgtatc
    10861 gcctggcgat gggctggcag gcgctgacag gcgttttgta acattgtgag atcggcgcag
    10921 gaaatctcgg tctcgacagc cggaagtggt tgatcattca gtaacgccac ggcgatgttg
    10981 gcggcgctgg caagtgtggt ggccgcggtg atattgggac gtgccagatc atgcggattc
    11041 gcaccgagga aattcaccac tacgggtttt ccacaagcct cggcacgctc cagaatagtc
    11101 cgggcaacga tcggtgaagg cggttttgat ataagcacaa tcacgcgagt ttcatcgtct
    11161 tgcgccagcg catctaaagc gaagagcatc gatattccac caatctcttc actgagatca
    11221 tgtccgcctg ttcccaacgc ctgagaaata cccgctccga gctggtcgat tcggcaagtc
    11281 acttcctgta gcccggtgcc tgacgcgcca ataacaccaa ttgctccgcg tttcaccaca
    11341 ttagcgaagc ctaacggaat gccgttaaca atggcggttc cacagtcagg tcccattacg
    11401 atgcgctgac gttctcgggc aagcgttttg atggattttt cctgcccgat actgacgtta
    11461 tcgctgaaca tcatcacgtt cattccgagg tttaacgctt tcattgcttc ggcggcggcg
    11521 tagtctcccg gtacagaaat gagtgccagg ttggcttcgg gttcggcttc cagtgccatt
    11581 tccaggctga ccttttccgg tgttttaatg ccactatccg tttcatcatc gggtttgctg
    11641 gtcagacgtt gctgggccag tgccagtgct tcattgcaaa tatcttcttc acccatgact
    11701 gcaatcacta aatcattggg actggcgttg atttcgttac ccagacccgc atcgcgcaat
    11761 tgttcgagat tattgggcgt tcccatgacg accgaggcct gttgaatacc gggcaacttg
    11821 ctgatttgcg cggaaatctg catcagagaa actgagtcct gataaagatt actgaaaact
    11881 ttatatttaa ctttcatgct gttacgcctg tttcattccg cccgatagca aaattcgggc
    11941 aaagcatccc cgtgtagccc cataaacggg ctacgggaga aaaggttgaa atcaattcat
    12001 ggtgttcagg gttcgaaagc catgcagcat tcctgcaagg caatctactc ctgacgttgc
    12061 gccaaattgc atgacctgtt ctgcacagga tgcgattgtc attgccgatg cgctcccaac
    12121 cagttgtgcg agtaactggc aaatcggttc tgaaaaatgt ccctgaagcg cacgctccag
    12181 ataatgacga ctgatatccg tggtgttgtg cgcatgttga tcaattgcct gctgcaaccg
    12241 gtattgatga tcggcaagtg gtgcagggag ttgccataac cagagcgctg ccagatagcc
    12301 caacaaatag tcatctccat cgggcgttaa gcctttacca aagccgatca gttgtggcac
    12361 ctggcgttcc agcgcatctg gatgctccat aatcgctaat ccctggtgcg tgagactatc
    12421 cggcaggtgc aatgtgcttt gaacgttatg ttccagacaa aataaaagcc gttgcgatgc
    12481 catcgttggg tagtgctgga aaaccaaagg gaatgcctca agtgtcagca ttctccccac
    12541 aaatggcgac tgccagagcg ttgcgttttc gagcgccaca caaaatcgag gtgtccgcag
    12601 taagccttgg gttaactgaa tgggatctga atgggcaatc tctcttcgcc agtcccaaca
    12661 ttcaggtagc cagactctgg cagcatcagg gagattcaga tacgtttgcg cacacaacag
    12721 agtgatgaat gttcccgcgt ctgaatacag atttaccgca tgtttaaaag cgctatgcag
    12781 acgtaaatct tgcgtttcat tctgcgggaa aagatatccc aacgacgatg cgcaaagcgt
    12841 aacctgtacc atcggcgtta gccttccaga cctgcaataa tggctttgga atcagtaacc
    12901 caaccgaaaa tggcaccttg tgctttgatc atctcgaggg cgtatttctg gaattccggg
    12961 aaataggagc cgacacaatc ttccgggata atgcattcat agccacgatc atttgcttcg
    13021 cgcacggtgg tggttacgca aacttcggtt gttacaccac aaactatcag cgtcttgata
    13081 ccgtggtttt gcaaaatcag atgtaagtcg gtttgataaa aagcgccttt accgggctta
    13141 tcaatcaccg gttcacctac taccgggtac agttcaggaa tgatgtcatg acccgcttca
    13201 cctctgacca gaatgcgtcc catcggtcct ggttcaccaa taaaggtttt tccgccgcgc
    13261 gtcagtttgg ctggcgggca gtcgcttaag tctgcacgat ggccttcacg agtatgaata
    13321 accataattc ctttttgacg tgcggctgcc agcacctctg tacatggtgc gatagccgtg
    13381 cgtaccagtg aaacatcatt gcccagtgcc tcaccgaagc cccctgcttc gacaaagtca
    13441 cgctgcatgt cgatcatgac cagggcagtt gtgcgcggat caaaaggtaa ttcaaagggt
    13501 tgagcctgaa aaatggattg cgtcatggta aatctcctgt ctgatgttta aacgatccag
    13561 ttgttctgag aacgagtatt caggagaaaa gcgacacaaa acaggaaaaa agtttgccgc
    13621 acagcaacga aatttttgca ttcagcaggt ggtgtgatga cgaatgtgaa gggagtcaaa
    13681 gagtggcata cagcaacaaa ttttttgatg ctgtatggtt ttgagattaa aaaaatcgtt
    13741 gaaattgtgt gagcttaatc atcagattta gcgactatgg cgttactttt taacaccata
    13801 ctgtgtaaac cattccaaca ttcgctgcca gccgtctttc gcagactcgg catgatagct
    13861 cggacgataa tcggcgttga acgcatgccc tgcatccggg tacacgataa tctctgcttt
    13921 cgcgtttgct gcccgcagcg cctggcgcat cgtttccacg ctctcttgcg gaatgctgtt
    13981 atcctggccg ccatataagc cgagaaccgg agcgttaaga tcggttgcga tatcaacagg
    14041 ttgtttcggt gaattcagcg acttatcgcc cgtcagtttg ccgtaccacg ccactgcggc
    14101 ttttagctgt ggattatgcg cggcatacag ccaggtgata cgtccacccc agcagaatcc
    14161 ggtgatcatt aaacgatgaa catcgccgcc gttgcgggaa gcccaactgg cgacatgatc
    14221 gagatcggcc agcacctgcg agtcaggcac ttttgctacc agaccgctaa gcaacgtggg
    14281 gatatcggca aaatcattcg gatcgccttc gcggaagtaa agttcaggtg cgatagccag
    14341 atacccctcc agcgccagac ggcgacaaat gtcgcggata tgttcatgca cgccaaaaat
    14401 ttcctgcact acaatgacca ctggcagtgg gccatcgctt tgctttggtc tggcatggta
    14461 agcaggcatg ttatcccctt gtgaagggat agaggtgaag cccgccacaa tcgcgtcgtc
    14521 cggagtctga acgatggtcg aagcgagagg cgatgcagca ggtgcaaatc cagattgttg
    14581 tgttgttgcc atggtattct ccgtaccctt ataaaaatgt tgcgcaatgt taactatagt
    14641 cagcatgcaa caaatcacat tgcctgaatc ggctcatctt ttatgcagtc ctgcagaatg
    14701 aagagtgatt tatgtgattt gcatcacttt tggcgggtaa atttatgcaa cgtattttcg
    14761 tcatggtgat gagtatcacg aaaaaatgtt aaacccttcg gtaaagtgtc tttttgcttc
    14821 ttctgactaa accgattcac agaggagttg tatatgtcca agtctgatgt ttttcatctc
    14881 ggcctcacta aaaacgattt acaaggggct acgcttgcca tcgtccctgg cgacccggat
    14941 cgtgtggaaa agatcgccgc gctgatggat aagccggtta agctggcatc tcaccgcgaa
    15001 ttcaccacct ggcgtgcaga gctggatggt aaacctgtta tcgtctgctc taccggtatc
    15061 ggcggcccgt ctacctctat tgctgttgaa gagctggcac agctgggcat tcgcaccttc
    15121 ctgcgtatcg gtacaacggg cgctattcag ccgcatatta atgtgggtga tgtcctggtt
    15181 accacggcgt ctgttcgtct ggatggcgcg agcctgcact tcgcaccgct ggaattcccg
    15241 gctgtcgctg atttcgaatg tacgacggcg ctggttgaag ctgcgaaatc cattggcgcg
    15301 acgactcacg ttggcgtgac agcttcttct gataccttct acccaggtca ggaacgttac
    15361 gatacttact ctggtcgcgt agttcgtcac tttaaaggtt ctatggaaga gtggcaggcg
    15421 atgggcgtaa tgaactatga aatggaatct gcaaccctgc tgaccatgtg tgcaagtcag
    15481 ggcctgcgtg ccggtatggt agcgggggtt atcgttaacc gcacccagca agagatcccg
    15541 aatgctgaga cgatgaaaca aaccgaaagc catgcggtga aaatcgtggt ggaagcggcg
    15601 cgtcgtctgc tgaaataaaa aaacgctccc caacttcggg gagcgatatc agttattgac
    15661 gccgtaaacg cattattttg cggattattg gcgtcagtag cgtaaaggcg gccagagtca
    15721 gcaatgccag agtgatcggt cgggtataca gaaagtcgag tgaaccccct gaaagcgaca
    15781 gcgcacggcg aaaattactt tccgccattg gccccataat caatgccagc actacgggtg
    15841 atgccggata tcctcctttc tgcatgaaat agcccaccac gcctgcaatc agcataatgc
    15901 caacatcaaa aaggctattg ttcaacgcat aagtcccgac cacacaaagt gcgaggatca
    15961 tcggtgtgag aatattgccg ggaattttca ccacattacc gatgactttc aaagagagca
    16021 gtccaagaac tagtagcatg aagtaacaga agatcatgcc gacaaacagc gtataaacca
    16081 tatcaccgtg gtcggtaaac atcagcgggc ctggttgcat tccctgtaac gttaacgcgc
    16141 ccagcatgat ggcggttacc gcatcgccag ggatccccag cgtcagcata ggtaacaatg
    16201 cgccaccggt gcagccattc gcgcctgcct cacaggaggc cacagcttta atttcaccct
    16261 taccaaagtt ttctggtgtc ttactgaaac gacgagtttc gttataggca acaaaagctg
    16321 caatatccgc ccccgcaccg gggatcatgc cgataaaaac ccctaatcct gatgagcgca
    16381 aaatggttaa tgcgatgcgg cgtagtgttt gccagggcag taacagactg ccgatagcca
    16441 ccttcgcgcc ccgacgaatt tgctggtttt gctccataga gcggaatgct tctgaagcag
    16501 caaataaacc aatcatcacc gggataaatg gcacattcat cagctccaca aatccacctg
    16561 taaaccgtgg aaaaccgccc attggatcaa ggcctatcgt tgcgatcagc aatccgccaa
    16621 ccccggcaat cagcccctta atcagcgact caccggaaat actggcaata atgctcagtc
    16681 cgaaggttgc cagggcgaag gactccgagg cactaaattt cagcgcccaa ctggcaagta
    16741 ccggagagag aaacatcagc acgataatgc tgagcgttcc gccactaaag gaagagaacg
    16801 tggcggcact caatgccagc cctgcttttc cctgtttcgt cagctcataa ccatcaatag
    16861 cggtggctgc ggcggcaggc gttccgggaa ttttgagcaa aattgcggtg atagaaccac
    16921 cgtagacgcc accaaaaaag acgccgcaaa tcatcaacaa tccagaaaca ggctccatgc
    16981 cgtaggtgaa aggcagcaaa atcgccacac ccatggtcgc tgtcagacca ggcaaagcgc
    17041 caatcacaat acccagcatc accccaaaag tggcaaacag cagcgcctga ggattattga
    17101 gtaatgtcga aaaaccctga gttaaaagtt cactttcaaa cattgctggc ccctgttatt
    17161 cgaaaaatgt gccgacaggc aatggcacct gtaacgcttc gacaaacagc aaccagagga
    17221 ttgccgttaa cagcagcgca atacccggtg tgagtaccca tcgcctgtag cccatcaacg
    17281 tcataatgac gatgaggaaa acaggagtag taatgagata accgcagtag ctcatcgcca
    17341 caatataaaa cgcggttgcc gcaattcctg ttgctgtttt cctgaatgcc ggtttttcct
    17401 gtgcgttggc gcaagaggct ggttttgcct gcaaaagatc gcgtatcacc agcaagacag
    17461 cgagcacaat cagggcacag gcatagaagg tagggaaggc acttgcaccg ggatccgttt
    17521 caatggcagt tgctggaaaa tcagcggcat taatcatcac cgcgccagca accacacaaa
    17581 gtaacagcgc caccaccatc gttgatgaaa ggcgcttgcg gtgcggtacg gcgagttgag
    17641 caacagactg ttttgtgttc atcaaccgta cctcaggttt attttttcag gcccaggcga
    17701 gtcagcaact cgtcaaagta cttttcctgt tcgctgattt gggtctggaa gctggcagcg
    17761 tcaagccatg catagttgag attcaacttt tgcagtgcat cctggaatgc aggctcttca
    17821 gccgtctctt ttgctgcctt tgccagaaca tccaccacat cctgcggcgt tttttgcgac
    17881 acaatcaggc cgcgccaggt gccgatggag agatcaacgc ctttctcttt taacgtcggg
    17941 acgtcaggca tggttttcat tcgctcatcc gccatcacta ccagtgtctt cagcttgccg
    18001 ccattcacat ggttgataac ttctcctggg cttacggaaa ccgcttcaat gtgcccacct
    18061 aacaggcctg taatggcagg ggctgcgccg tcatacggga catgagaaaa ctttgcgccc
    18121 gttttgtctt ccagtgcagc tgccgccaga tgccagatag ctccggtgcc tgagttacca
    18181 atgcgtactt ttccgggatt cgctttggcg taagtcataa attcttcata gctattccac
    18241 ggcgcatcgg cacggactgt gatagcagcc ggatccgcat tcagacgggc aatgggttta
    18301 aagtcgctgg ttttaaaacg caccattccg aggctgggaa gggtggttag ttcaaccgtg
    18361 cctaaaccaa ttttgtaacc gttagggcgg gcagcggcga tttcactcag gccgatagca
    18421 ccgcctccgc caggtttatt gataaccccg atgctgacgg gtaaatggtt tttggcggca
    18481 tcagcaaaag ctcgggcaac cagatccgta ccgcctccgg cagcgtaggg tacgactaac
    18541 tcgatctgtt tggttggcca ggaagccgcc tgtacaggcg caatgctgag ggcagcgcct
    18601 gcaagaagta ccattgcagc catttttttc ggtaaggtaa gatgttttat cattgctttt
    18661 atatccttat ttatcagtta gttgctattt aaattgttcc atgcgttcat aaacagtact
    18721 gcattttcat agacttgctc tggcgtcatg ccggggtgat atagcgcaga accgaggcca
    18781 aagccgtttg cgccgtggct cagataacgc gccatggttt ccggtgtgat accgccaacc
    18841 ggtaacatcg gaacggattg tggaataacc gcacgccagg ctttggtaac ttctggcgta
    18901 atttgttcag ccgggaacag cttgatggca ttagcaccat gttcaatggc agcaaaggct
    18961 tcggtgggcg tggcgacgcc aggcgcacaa tacataccca gcgcacgggc gcgacggatc
    19021 accgcagtat cgcaatgcgg catcacgata atctggcccc cagcctcttt aaccgcaacc
    19081 acctgctcta ccgaaagcac agttccggct ccgaccaggc agtccttagg cagggcgtcg
    19141 cgcagtaaac tgatactttg caacgcctcc ggcgagttca taggaatctc aatcaggcgg
    19201 aatccggcgg cgtacagttt ttcaccgatg gcgacaatct catccggttt cacaccgcgt
    19261 aaaatggcga ctaacccggt tttttgcaaa agcgtattaa atggcatggg tacattccct
    19321 ggcgttggtg gacagtaacc cggcagcgtg ggcgattcgc cataatccct gttcagtggc
    19381 attgggaatg gcgtgtatct gtctgtcggg aaaaacgtgg ctgaatgctt gtgaatagcg
    19441 gctgttcaga tttgcgctac cgattaaggc gatcggtgtt gtggcgggta aatcacgtag
    19501 caaactggat gccagaccac ataattcatg gcctatcagt aatccggaaa ggtaatcccc
    19561 ctgcgaggac gacgtaagat tactggtcag caggcgcgaa cgggtcgaaa acagcgtcga
    19621 taataatccg gtctgatgat gctcttgtgc tgctgtcagc ccgtgggtga aggcatccca
    19681 gtgcggttca tctccaggat gcatggtggc accgagaatc gaatggcggg ataaaacatc
    19741 aaataactcg cccgtcataa aggttcggaa atcggtaatg gtgttgtttt caactactgc
    19801 ccatttcgca tgtgtgccgg gtaagcctat cagcactggc ataccctgat ggatcgcgtt
    19861 ttgcagggcc ggttccatcg agatagcacc gaagatttgt gtctcttcgc cacgcatcac
    19921 ttcgggcatt tcaccttctt taatcacgcc aggaatgatg tgcagcattg tctgctgctg
    19981 gtggataaca ggcgtcaact gcttcgccag accctcgaga gtgaccgggc aaggtaaata
    20041 agctgctgat tgccaccctt gcgcactgcc gagcatgccg caagccagta ccgggcatgg
    20101 ggtttgtgcc agccagtcgc cgcagacacg taaaaacgta tcgtggaaag cgtcctcacg
    20161 tgttgctgcc tgggagggta atttcatgat cccccagggg aattcacgtt tttcttgtgg
    20221 atttggtgat tcgccaaaac gccatgcccg aagcgatgac gttccccagt ccagggcaat
    20281 aaatgagggt gctcccggca ttagtcgttt cctttaatca tggtttattt atctttgcgg
    20341 acgtgcttac cccagcccag ttcagcggat atctcttctg cacaggattt gacgtaaggt
    20401 gccagttctt ccagacgcgc cagcgacata tacgtggtgg tactggcgac agaaatagcc
    20461 gccacaattt catcccgggc gttgtaaacc ggcgctgcaa cgcaacggat agtcggctcg
    20521 ttctcttcca gatcaaaagc aaaaccactg gcggcataac gacgcatgtt ctgaataaat
    20581 gcgcccagtt ttgtttcatc accacaggtc ttaaacagcg tgcgccactc ttcttctgtt
    20641 cggttgagga tcaatgcctt gccgatgccg gtgattgcca gtggcatgcg atggcctggg
    20701 cgtgagcgca tttccagccc gcgctgactg ttgatcttct ccagatacag cacttcgtcg
    20761 ccttcaacga tccccagatg cacggtgtcc agggtgtaac gggcaaggcg ttgcaaaacg
    20821 ggctgggcaa tttcgtacag cgaggtgctt tccagtgctc tggcaccaaa ttcgatcagt
    20881 ttggcaccca gttggtagcc ctgcgttggg acatggcgca ggtagcgttg ttccaccaga
    20941 acggtgacta aacgatgggt tgtgctccgg gtcgtgccgg tgaactcgcc gatacttttt
    21001 aggtcatggc agccgttata aacagcatta agaaccgcca gtccacgtaa taacgtttgt
    21061 gcgcctgcgg tggttgaaga ggtttctgcc atacgtttct cctgatgata ttgctgaagt
    21121 gttccacata atgacgtggg gaaaaggtga tatatcccac aatataaact ccatctcata
    21181 ttgtgggata aaataatgaa aattaacgat cgttttgcgt agcgcctcgc aggaaacttc
    21241 tttcaaactg gacgtttgta cagcacaatt ctattttgtg cgggtaagtt gttgcgtcag
    21301 gaggcgttgt ggatttctca atcatggttt acgcagttat tgcattggtg ggtgtggcaa
    21361 ttggctggct gtttgccagt tatcaacatg cgcagcaaaa agccgagcaa ttagctgaac
    21421 gtgaagagat ggtcgcggag ttaagcgcgg caaaacaaca aattacccaa agcgagcact
    21481 ggcgcgcaga gtgtgaattg ctcaataacg aagtgcgcag cctgcaaagt attaacacct
    21541 cgctggaggc cgatctgcgt gaagtgacca cgcggatgga agccgcacag caacacgctg
    21601 acgataaaat tcgccagatg atcaatagcg aacagcgcct cagtgagcaa tttgaaaacc
    21661 ttgccaaccg tatttttgaa cacagtaatc gtcgggttga tgagcaaaat cgccagagtc
    21721 tgaacagtct tttgtcgccg ttgcgtgaac aactggatgg tttccgccgt caggtccagg
    21781 acagctttgg taaagaagcc caagaacgtc ataccctgac tcacgaaatc cgcaacctcc
    21841 agcaactcaa cgcccagatg gcccaggaag cgatcaacct gacgcgtgcg ctgaaaggcg
    21901 acaataaaac ccagggcaac tggggcgagg tagtattgac gcgggtgctg gaggcttccg
    21961 gtctgcgtga agggtatgaa tatgaaaccc aggtcagcat cgaaaatgac gcccgctcgc
    22021 ggatgcagcc ggatgtcatc gtgcggttgc cgcagggaaa agatgtggtg atcgacgcca
    22081 aaatgacgct ggtcgcctat gaacgctatt tcaacgccga agatgactac tcccgcgaaa
    22141 gcgcgctaca ggaacatatc gcgtcggtac gtaaccatat ccgtttgctg ggacgcaaag
    22201 attatcaaca gctgccgggg ctgcgaactc tggattacgt gctgatgttt attcccgttg
    22261 aacccgcttt tttgctggcg cttgaccgtc agccggagct gatcaccgaa gcattgaaaa
    22321 acaatatcat gctggttagc ccgactacgc tgctggtggc gctgcgcact atcgccaacc
    22381 tgtggcgtta tgagcatcaa agccgcaacg cccagcaaat cgccgatcgt gccagcaagc
    22441 tgtacgacaa gatgcgtttg ttcatcgatg acatgtccgc gattggtcaa agtctcgaca
    22501 aagcgcagga taactatcgc caggcaatga aaaaactctc ttcaggtcgc ggaaatgtgc
    22561 tggcgcaggc agaagcgttt cgcggtttag gggtagaaat taaacgcgag attaatccgg
    22621 atttggctga acaggcggtg agccaggatg aagagtatcg acttcggtcg gttccggagc
    22681 agccgaatga tgaagcttat caacgcgatg atgaatataa tcagcagtcg cgctagccca
    22741 ttgggggtag ttaagccggg tagaaatcta gggcatcgac gcccaatctg ttacacttct
    22801 ggaacaattt tttgatgagc aggcattgag atggtggata agtcacaaga aacgacgcac
    22861 tttggttttc agaccgtcgc gaaggaacaa aaagcggata tggtcgccca cgttttccat
    22921 tccgtggcat caaaatacga tgtcatgaat gatttgatgt cattcggtat tcatcgtttg
    22981 tggaagcgat tcacgattga ttgcagcggc gtacgccgtg ggcagaccgt gctggatctg
    23041 gctggtggca ccggcgacct gacagcgaaa ttctctcgcc tggtcggaga aactggcaaa
    23101 gtggtccttg ctgatatcaa tgaatccatg ctcaaaatgg gccgcgagaa gctgcgtaat
    23161 atcggtgtga ttggcaacgt tgagtatgtt caggcgaacg ctgaggcgct gccgttcccg
    23221 gataacacct ttgattgcat caccatttcg tttggtctgc gtaacgtcac cgacaaagat
    23281 aaagcactgc gttcaatgta tcgcgtgctg aaacccggcg gccgcctgct ggtacttgag
    23341 ttctcgaagc caattatcga gccgctgagc aaagcctatg atgcatactc cttccatgtg
    23401 ctgccgcgta ttggctcact ggtcgcgaac gacgccgaca gctaccgtta tctggcagaa
    23461 tccatccgta tgcatcccga tcaggatacc ctgaaaacca tgatgcagga tgccggattc
    23521 gaaagtgtcg actactacaa tctgacggca ggggttgtgg cgctgcatcg tggttataag
    23581 ttctgacagg agaccggaaa tgccttttaa acctttagtg acggcaggaa ttgaaagtct
    23641 gctcaacacc ttcctgtatc gctcacccgc gctgaaaacg gcccgctcgc gtctgttggg
    23701 caaagtgctg cgcgtggagg taaaaggctt ttcgacgtca ttgattctgg tgttcagcga
    23761 acgccaggtc gatgtactgg gcgaatgggc aggcgatgct gactgcaccg ttatcgctta
    23821 cgccagcgtg ttgccgaaac ttcgcgatcg ccagcagctt gccgcactga ttcgcagtgg
    23881 tgagctggaa gtgcagggtg atattcaggt ggtgcaaaac ttcgttgcgc tggcagatct
    23941 ggctgagttc gaccctgcgg aactgctggc cccttatacc ggtgatatcg ccgctgaagg
    24001 tatcagcaaa gccctgcgcg ggggcgcaaa gttcctgcat cacggcatta agcgccagca
    24061 acgttatgtg gcggaagcca ttactgaaga gtggcgtatg gcacccggtc cgcttgaagt
    24121 ggcctggttt gcggaagaga cggctgccgt cgagcgtgct gttgatgccc tgaccaaacg
    24181 gctggaaaaa ctggaggcta aatgacgcca ggtgaagtac ggcgcctata tttcatcatt
    24241 cgcacttttt taagctacgg acttgatgaa ctgatcccca aaatgcgtat caccctgccg
    24301 ctacggctat ggcgatactc attattctgg atgccaaatc ggcataaaga caaaccttta
    24361 ggtgagcgac tacgactggc cctgcaagaa ctgggaccgg tatggatcaa gttcgggcag
    24421 atgttatcaa cccgccgcga tctttttccg ccgcatattg ccgatcagct ggcgttattg
    24481 caggacaaag tcgctccgtt tgatggcaag ctggcgaagc agcagattga agctgcaatg
    24541 ggcggcttgc cggtagaagc gtggtttgac gattttgaaa tcaagccgct ggcttctgct
    24601 tctatcgccc aggttcatac cgcgcgattg aaatcgaatg gtaaagaggt ggtgattaaa
    24661 gttatccgcc cggatatttt gccggtcatt aaagcggatc tgaaacttat ttaccgtctg
    24721 gctcgctggg tgccgcgttt gctgccggat ggtcgccgtc tgcgcccaac cgaagtggtg
    24781 cgcgagtacg aaaagacctt gattgatgaa ctgaatttgc tgcgggaatc tgccaacgcc
    24841 attcagcttc gacgcaattt tgaagacagc ccgatgctct acatcccgga agtttaccct
    24901 gactattgta gtgaagggat gatggtgatg gagcgtattt acggcattcc ggtgtctgat
    24961 gttgcgacgc tggagaaaaa cggcaccaac atgaaattgc tggcggaacg cggcgtgcag
    25021 gtgttcttca ctcaggtctt tcgcgacagc tttttccatg ctgatatgca ccctggcaac
    25081 atcttcgtaa gctatgaaca cccggaaaac ccgaaatata tcggcattga ttgcgggatt
    25141 gttggctcgc taaacaaaga agataaacgc tatctggcgg aaaactttat cgccttcttt
    25201 aatcgcgact atcgcaaagt ggcagagcta cacgtcgatt ctggttgggt gccaccagat
    25261 accaacgttg aagagtttga atttgccatt cgtacggtct gtgaacctat ctttgagaaa
    25321 ccgctggccg aaatttcgtt tggacatgta ctgttaaatc tgtttaatac ggcgcgtcgc
    25381 ttcaatatgg aagtgcagcc gcaactggtg ttactccaga aaaccctgct ctacgtcgaa
    25441 ggggtaggac gccagcttta tccgcaactc gatttatgga aaacggcgaa gcctttcctg
    25501 gagtcgtgga ttaaagatca ggtcggtatt cctgcgctgg tgagagcatt taaagaaaaa
    25561 gcgccgttct gggtcgaaaa aatgccagaa ctgcctgaac tggtttacga cagtttgcgc
    25621 cagggcaagt atttacagca tagtgttgat aagattgccc gcgagcttca gtcaaatcat
    25681 gtacgtcagg gacaatcgcg ttattttctc ggaattggcg ctacgttagt attaagtggc
    25741 acattcttgt tggtcagccg acctgaatgg gggctgatgc ccggctggtt aatggcaggt
    25801 ggtctgatcg cctggtttgt cggttggcgc aaaacacgct gattttttca tcgctcaagg
    25861 cgggccgtgt aacgtataat gcggctttgt ttaatcatca tctaccacag aggaacatgt
    25921 atgggtggta tcagtatttg gcagttattg attattgccg tcatcgttgt actgcttttt
    25981 ggcaccaaaa agctcggctc catcggttcc gatcttggtg cgtcgatcaa aggctttaaa
    26041 aaagcaatga gcgatgatga accaaagcag gataaaacca gccaggatgc tgattttact
    26101 gcgaaaacta tcgccgataa gcaggcggat acgaatcagg aacaggctaa aacagaagac
    26161 gcgaagcgcc acgataaaga gcaggtgtaa tctgtgtttg atatcggttt tagcgaactg
    26221 ctattggtgt tcatcatcgg cctcgtcgtt ctggggccgc aacgactgcc tgtggcggta
    26281 aaaacggtag cgggctggat tcgcgcgttg cgttcactgg cgacaacggt gcagaacgaa
    26341 ctgacccagg agttaaaact ccaggagttt caggacagtc tgaaaaaggt tgaaaaggcg
    26401 agcctcacta acctgacgcc cgaactgaaa gcgtcgatgg atgaattacg ccaggcagcg
    26461 gagtcgatga agcgttccta cgttgcaaac gatcctgaaa aggcgagcga tgaagcgcac
    26521 accatccata acccggtggt gaaagacaat gaaactgcgc atgaaggcgt aacgcctgct
    26581 gctgcacaaa cgcaggccag ttcgccggaa cagaagccag aaaccacgcc agagccggtg
    26641 gtaaaacctg ctgcggacgc tgaaccgaaa accgctgcac cttccccttc gtcgagtgat
    26701 aaaccgtaaa catgtctgta gaagatactc aaccgcttat cacgcatctg attgagctgc
    26761 gtaagcgtct gctgaactgc attatctcgg tgatcgtgat attcctgtgt ctggtctatt
    26821 tcgccaatga catctatcac ctggtatccg cgccactgat caagcagttg ccgcaaggtt
    26881 caacgatgat cgccaccgac gtggcctcac cgttctttac gccgatcaag ctgaccttta
    26941 tggtgtcgct gattctgtca gcgccggtca ttctctatca ggtgtgggcg tttatcgccc
    27001 cagcgctgta taagcatgaa cgtcgcctgg tggtgccgct gctggtttcc agctctctgc
    27061 tgttttatat cggcatggcg ttcgcctact ttgtggtctt tccgctggca tttggcttcc
    27121 ttgccaatac cgcgccggaa ggggtacagg tatccaccga cattgcgagc tatttaagct
    27181 tcgttatggc gctgtttatg gcgtttggtg tctcctttga agtgccggtg gcaattgtgc
    27241 tgctgtgctg gatggggatt acctcgccag aagacttacg caaaaaacgc ccgtatgtgc
    27301 tggttggtgc attcgttgtc gggatgttgc tgacgccgcc ggatgtcttc tcgcaaacgc
    27361 tgttggcgat cccgatgtac tgcctgtttg aaatcggtgt cttcttctca cgcttttacg
    27421 ttggtaaagg gcgaaaccgg gaagaggaaa acgacgctga agcagaaagc gaaaaaactg
    27481 aagaataaat tcaaccgccc gtcagggcgg ttgtcatatg gagtacagga tgtttgatat
    27541 cggcgttaat ttgaccagtt cgcaatttgc gaaagaccgt gatgatgttg tagcgcgcgc
    27601 ttttgacgcg ggagttaatg ggctactcat caccggtacc aatctgcgtg aaagccagca
    27661 ggcgcaaaag ctggcgcgtc agtattcgtc ctgttggtca acggcgggcg tacatcctca
    27721 cgacagcagc cagtggcaag ctgtgactga agaagcgatt attgagctgg ccgcgcagcc
    27781 agaagtggtg gcgattggtg aatgtggtct cgactttaac cgcaactttt cgacgccgga
    27841 agagcaggaa ctcgcttttg ttgcccagct acgcattgcc gcagaattaa acatgccggt
    27901 atttatgcac tgtcgcgatg cccacgagcg gtttatgaca ttgctggagc cgtggctgga
    27961 taaactgcct ggtgcggttc ttcattgctt taccggcaca cgcgaagaga tgcaggcgtg
    28021 tgtggcgcgt ggaatttata tcggcattac cggttgggtt tgcgatgaac gacgcgggct
    28081 ggagctgcgg gaattgttgc cgttgattcc ggcagagaaa ttgctgatcg aaactgatgc
    28141 gccgtatctg ctccctcgcg atctcacgcc aaagccatca tcccggcgca acgagccagc
    28201 ccatctgccc cgtattttgc aacgtattgc gcactggcgt ggagaagatg ccgcatggct
    28261 ggctgccacc acggatgcta atgtcaaaac actgtttggg attgcgtttt agagtttgcg
    28321 gaactcggtg ttcttcacac tgtgcttaat ctctttatta ataagattca gcaatagcat
    28381 ggagcgagcc tcgccatcgg gttcggtgaa aatggcctga aagccttcga acgcgccttc
    28441 agtaataatc actttatcac caggatacgg cgtcgatgga tcgacaatat ctttcggttt
    28501 atacactgat aactgatgaa tcaccgccga tgggactatc gctggcgacg cgccaaagcg
    28561 cacgaagtgg ctgacaccgc gggtcgcgtt gatagtcgtg gtatgaatca cttccggatc
    28621 gaactccaca aacaagtagt tggggaacaa tggctcactg actgcagtac gtttgccacg
    28681 cacgattttt tccagggtga tcatcggtgc caggcaattc acagcctgtc tttcgaggtg
    28741 ttcctgggca cgttgaagtt gcccgcgctt gcagtacagt aaataccagg attgcataat
    28801 gactcttatc cgtttaatcg gggcgcaagg atagcaaaag ctttacgcta agttaattat
    28861 attccccggt ttgcgttata ccgtcagagt tcacgctaat ttaacaaatt tacagcatcg
    28921 caaagatgaa cgccgtataa tgggcacaga ttaagaggct acaatggacg ccatgaaata
    28981 taacgattta cgcgacttct tgacgctgct tgaacagcag ggtgagctaa aacgtatcac
    29041 gctcccggtg gatccgcacc tggaaatcac tgaaattgct gaccgcactt tgcgtgccgg
    29101 tgggcctgcg ctgttgttcg aaaaccctaa aggctactca atgccggtgc tgtgcaacct
    29161 gttcggtacg ccaaagcgcg tggcgatggg catggggcag gaagatgttt cggcgctgcg
    29221 tgaagttggt aaattattgg cgtttctgaa agagccggag ccgccaaaag gtttccgcga
    29281 cctgtttgat aaactgccgc agtttaagca agtattgaac atgccgacaa agcgactgcg
    29341 tggtgcgccc tgccaacaaa aaatcgtctc tggcgatgac gtcgatctca atcgtattcc
    29401 cattatgacc tgctggccgg aagatgccgc gccgctgatt acctgggggc tcaccgtaac
    29461 gcgcggcccg cataaagagc ggcagaatct gggcatttat cgccagcagc taattggtaa
    29521 aaacaaactg attatgcgct ggctgtcgca tcgcggcggc gcgttggatt atcaggagtg
    29581 gtgtgcggcg catccgggcg aacgtttccc ggtttctgtg gcgctgggtg ccgatcctgc
    29641 cacgattctc ggtgcagtca cccccgttcc ggatacgctt tcagagtatg cgtttgccgg
    29701 attgctgcgt ggcaccaaga ccgaagtggt gacgtgtatc tccaatgacc ttgaagtgcc
    29761 cgccagtgcg gagattgtgc tggaagggta tatcgaacaa ggcgaaactg cgccggaagg
    29821 gccgtatggc gaccacaccg gttactataa cgaagtcgat agtttcccgg tatttaccgt
    29881 gacgcatatt acccagcgtg aagatgcgat ttatcattcc acctataccg ggcgtccgcc
    29941 agatgaacct gcggtactgg gcgtagcact gaacgaagtg ttcgtgccga ttctgcaaaa
    30001 acagttcccg gaaattgtcg atttttatct gccgccggaa ggctgttctt atcgcctggc
    30061 ggtagtgacg atcaaaaaac agtatgccgg acacgcgaag cgcgtcatga tgggcgtctg
    30121 gtcgttctta cgccagttta tgtacactaa atttgtgatc gtttgcgatg atgacgtcaa
    30181 cgcccgcgac tggaacgatg tgatttgggc gattaccacc cgtatggacc cggcgcggga
    30241 taccgttctg gtagaaaata cgcctattga ttatctggat tttgcctccc ctgtctccgg
    30301 gctgggttca aaaatggggc tggatgccac gaataaatgg ccgggggaaa cccagcgtga
    30361 atggggacgt cccatcaaaa aagatccaga tgttgtcgcg catattgacg ccatttggga
    30421 tgaactggct atttttaaca acggtaaaag cgcctgatgc gcgtttgttt tgccctattt
    30481 atcgatccga cagagaaagc gcatgacaac cttaagctgt aaagtgacct cggtagaagc
    30541 tatcacggat accgtatatc gtgtccgcat cgtgccagac gcggcctttt cttttcgtgc
    30601 tggtcagtat ttgatggtag tgatggatga gcgcgacaaa cgtccgttct caatggcttc
    30661 gacgccagat gaaaaagggt ttatcgagct gcatattggc gcttctgaaa tcaaccttta
    30721 cgcgaaagca gtcatggacc gcatcctcaa agatcatcaa atcgtggtcg atattcccca
    30781 cggagaagcg tggctgcgcg atgatgaaga gcgtccgatg attttgattg cgggcggcac
    30841 cgggttctct tatgcccgct cgattttgct gacagcgttg gcgcgtaacc caaaccgtga
    30901 tatcaccatt tactggggcg ggcgtgaaga gcagcatctg tatgatctct gcgagcttga
    30961 ggcgctttcg ttgaagcatc ctggtctaca agtggtgccg gtggttgaac aaccggaagc
    31021 gggctggcgt gggcgtactg gcaccgtgtt aacggcggta ttgcaggatc atggtacgct
    31081 ggcagagcat gatatctata ttgccggacg ttttgagatg gcgaaaattg cccgtgacct
    31141 gttttgcagc gagcgtaatg cgcgggaaga tcgcctgttt ggcgatgcgt ttgcatttat
    31201 ctgagatata aaaaacccgc ccctgacagg cgggaagaac ggcaactaaa actttataca
    31261 gtggcattac tctacgttgc atccggcaaa cagtgcccga tgcaacgctg ccgcgtctta
    31321 tcgggcctac aaaagttctg aaccgtgggc cggataaggc gttcacgccg tatccggcat
    31381 acagtgcccg atgcgacgct gccgcgtctt atcgggccta caaaagttct gaaccgtagg
    31441 ccggataagg cgttcacgcc gcatccggca agtggttaaa cccgctcaaa caccgttgca
    31501 atgccctgac ccagaccgat acacatcgtc gccagaccaa actggacgtc tttgtgttcc
    31561 atcagattca gcagcgtggt gctgatacgc gcaccggaac aacccagcgg atgacccagc
    31621 gcgatcgcgc caccgttgag gttgatcttc tcgtcaatct gctccattag cccgagatct
    31681 ttaatacacg gcaggatctg cgcggcaaag gcttcgttca tctcgaatac gccgatatcg
    31741 ctggcagaaa gccccgcttt tttcagcgcc agcttcgagg ccggaaccgg accataaccc
    31801 ataatcgatg ggtcacaacc cacaaccgcc atcgaacgca cgcgggcgcg cggcttaaga
    31861 cctaattcac gggcgcggct ttcactcatc accagcatgg cagctgcgcc atcggaaagc
    31921 gcagaagatg tacccgccgt taccgtaccg ctgacgggat caaacgccgg acgcagtgtg
    31981 gcgagggctt ccacggtggt ttccgggcga atcacttcgt cgtaattaaa ctgcttcaat
    32041 acgccgtcgg catcgtgacc accggtcggg atgatttcat ttttaaatgc gcctgactgc
    32101 gtggcggccc acgcgcgggc gtgtgaccgc gcggcaaagg catcctgcat ttcacggctg
    32161 ataccgtgca tacgcgccag catttctgcc gttaagccca tcatgcccgc cgctttggcg
    32221 acattgcggc tcaggccggg gtgaaaatcg acgccgtgac tcattggcac atggcccata
    32281 tgctccacgc cgccaaccag acatgcctgc gcatcgccgg tcatgatcat tcgtgctgcg
    32341 tcatgcagtg cctgcatgga tgaaccacac aagcgattaa cggtaaccgc cgggacagag
    32401 tgcggcactt cagccaacag tgccgcgtta cgggcgatat taaaaccctg ctccagtgtt
    32461 tgctgcacac aaccccagta aatatcgtca agggccgccg cttccagtgc cgggttacgc
    32521 gccagcaggc tacgcattaa atgagcggag agatcttccg cacgtacgtt acgaaaagca
    32581 ccgcccttcg aacggcccat cggggtgcga attgcatcga caatgacaac ctgttccatt
    32641 gtgactcctt aagccgtttt caggtcgcca accggacggg ctggctcaac cgggggatag
    32701 tactgttcgt tatgacgtgc tttattacgc agaccttccg gtacttcata cagcgggccg
    32761 aggtgctgat attgctgtgc catatcgagg tattttgcgc tgccgagggt gtccagccag
    32821 cggaacgcgc cgccgtggaa cggagggaag cccaggccgt agaccagcgc catatccgct
    32881 tctgccggag tggcgataat gccctcttcc agacagcgga ccacttcgtt gaccatcggg
    32941 atcatcatgc gggcgataat ctcttcttcg ctgaaatcgc gcttcggctg gctcacttct
    33001 gccagcaggt cgtcaacagc ggcgtcttct cctttcttcg gcttaccttt gctgtcttct
    33061 ttataacgcc agaaaccgag gccgttcttc tgaccaaagc ggttggcatt aaacagcgcg
    33121 tcgatggcat cgcgataatc tttctgcatc cgctgcggga agcctgctgc catgactgcc
    33181 tgggcatgat gtgcggtgtc aatgcccaca acatccagca gatatgccgg acccatcggc
    33241 cagccaaact gtttttccat cactttgtcg atcttgcgga aatccgcgcc gtcgcgcagc
    33301 aactggctga atccggcgaa gtaggggaac agtacacggt taacgaagaa gccggggcag
    33361 tcgttgacca caatcggcgt cttgcccatc ttgctcgccc atgcgacgac tttcgcgatg
    33421 gtttcgtcgg agcttttctc gccgcgaata atttccacca acggcatccg gtgcaccggg
    33481 ttaaagaagt gcatcccgca gaagttttcc ggacgttcca gcgcgttggc cagttcgctg
    33541 ataggaatgg ttgaagtgtt agacgccagc acggtatccg ggcgtacttt ctgctcggtt
    33601 tctgccagca cggctttttt cactttcggg ttttcaacaa ccgcttctac cacaacatcc
    33661 acgcggtcaa atccggcgta gtcgagcgtt gggtggattg tggagatcac gccagccagt
    33721 ttcagaccat cgatcttgcc gcgttcaagc tgcttgttca gcagtttcgc ggcttcggtc
    33781 atgccgaggg ttaacgactt gtcgttgata tctttcatga taaccggcac acctttccac
    33841 gcagactggt aagcgatgcc gccgcccata atgcctgcgc ccagcacagc ggcctgtttc
    33901 ggcgtttcaa cgtctttggt gagtttcttc gctttgcctt ttacatattg atcgttaagg
    33961 aatatgccga ccagtgcgcg agcttcgttg gtatgcgcca gcgggacaaa acttttgttt
    34021 tccaggttta gggcttcttc acgaccaaaa cgggccgcag cttcaatggt ttttactgca
    34081 gtgatggggg ccggataatg tttccccgcc gtttgcgcga ccatcccttt agcgatggtg
    34141 aagctcatgg tggcttcaat cttgctcagt tttagcggtt ccagcttcgg ctgacgtttt
    34201 gctttccagt cgaggtcgcc gttaatggcc tgacgtaaaa tcgccttcgc gccttcaacc
    34261 agtttttctg ctttgactac gccatccacc agaccgattt tcagcgcctg atccgcgccg
    34321 acatctttac cggcggcaat gatttccagc gcactgtcag cgcccagcat acgtggcata
    34381 cgtacagaac cgccaaagcc cggcatgatg cccagtttgg tttccggcag accgatgcgc
    34441 agatccggcg tcgccagacg ataatcggtc gccagcacgc attcgcagcc accgcccagc
    34501 gcatagccgt tgacggcagc aatggtcggc accggcagat cttccaggcg attaaacacg
    34561 ctattggcaa agtgcagcca ctgacttaac tgttcttcag gaacgaggaa cagggacaaa
    34621 aattcggtga tatcagcacc gacgataaag gctgctttgt tcgaacgtag cagcagtcct
    34681 tttagatctg attgctgttc cagcacgccg atggcctcgc cgaggctggc gacggtcgca
    34741 gtgtcgagtt tattaactga acctggggta tcaaatacca gttcggcaat gccatcttcc
    34801 agccagtcaa ggtacagggt gtcgcctttg taaagcatgt cagtctcctg aatccgcaag
    34861 gtgatctggt cgtaccagat gcatcgaagt gtgtattttg tgttaaaaat atgcaaataa
    34921 aatattaagg aaatgccgat ctgatcacgc tcggcagaaa tcacgctctg gatgaacgat
    34981 gtgctaatat gcggagactt aaggtcaaaa aaacagaagg gtaaaaaatg gaatcactgg
    35041 cctcgctcta taaaaatcat atagctacct tacaggaacg gactcgcgat gcgctggcgc
    35101 gcttcaagct ggatgcgtta cttattcact ccggcgaact gttcaacgtt tttctcgacg
    35161 atcatcccta tccgtttaaa gtgaacccgc aattcaaagc gtgggtgccg gtaactcagg
    35221 tgccaaactg ctggctgctg gtggatggcg tgaataagcc gaaactgtgg ttttatctgc
    35281 cggttgatta ctggcacaac gtcgaaccgc tgccgaactc cttctggact gaagatgtag
    35341 aagtgatcgc gctgccaaaa gccgatggca ttggtagtct gttgcctgct gcgcgcggca
    35401 atatcggtta tatcggtccg gtgccagaac gtgcgctgca actgggtatt gaggccagca
    35461 atatcaaccc gaaaggggtg atcgactacc tgcattacta tcgctccttc aaaaccgagt
    35521 acgaactggc ctgtatgcgt gaagcgcaga aaatggcggt caacggtcat cgcgcggcag
    35581 aagaagcgtt ccgttctggc atgagcgagt tcgatatcaa cattgcctat ctgaccgcga
    35641 ccggtcatcg tgataccgac gtaccttata gcaacattgt ggcgcttaac gaacacgctg
    35701 cggtgttgca ttacaccaaa ctggaccacc aggcaccgga agagatgcgc agcttcctgc
    35761 tggatgccgg ggccgaatat aacggctatg cggctgatct aacccgtacc tggtcggcaa
    35821 aaagcgacaa cgactacgca cagctggtga aagacgtaaa tgatgagcaa ctggcgctga
    35881 tcgccaccat gaaagcaggc gtcagctatg tggattacca tatccagttc catcagcgca
    35941 tcgccaaact gctgcgtaaa catcaaatca tcaccgatat gagtgaagaa gcgatggtcg
    36001 aaaacgatct caccggaccg tttatgccgc acggtatcgg ccatccgctg ggcctgcagg
    36061 tgcatgacgt agccggtttt atgcaggatg atagcggtac gcacctcgcg gcaccggcaa
    36121 aatatccgta cctgcgctgc acccgtattc tccagccggg catggtgtta accatcgaac
    36181 cgggtatcta cttcatcgaa tcgcttctgg caccgtggcg tgaagggcag ttcagcaaac
    36241 acttcaactg gcagaaaatt gaagcactga aaccgttcgg cggcattcgt atcgaagaca
    36301 acgtggtgat tcacgaaaac aacgtggaaa acatgacccg ggatctgaaa ctggcgtgat
    36361 ggaaagctgg ttaattcctg cggcaccggt cacggtcgtt gaagagatca aaaagagccg
    36421 cttcattacg ctgttggcgc ataccgatgg cgttgaggcg gcaaaagcgt ttgttgaatc
    36481 ggtgcgggca gaacaccccg atgcccggca ccattgcgtg gcgtgggtgg cgggtgcgcc
    36541 ggatgattct caacagctgg gtttctctga cgacggggag ccggcgggaa cggcaggtaa
    36601 accgatgctc gcccaactaa tgggcagcgg cgtcggggaa attaccgctg tggtggtgcg
    36661 ctactacggc ggcatattgc ttggcaccgg tgggttagtg aaagcgtatg gcggcggcgt
    36721 gaatcaggcg ctgcgccagc taacgaccca acgcaagaca ccattaaccg aatatacttt
    36781 gcaatgtgaa tatagtcagt taaccggcat tgaagcgttg ctggggcagt gtgacggcaa
    36841 aattatcaac agtgattatc aggcattcgt tctgctgcgg gtggcgcttc cggcggcgaa
    36901 agtggctgaa ttttccgcaa agctggcgga ttttagccgt ggttcattgc aattgttagc
    36961 gattgaagaa taatccccac ttcgttttgc agaactaagg aagcggcaga gatgcatttt
    37021 cgcgccatta cccgaatcgt tggactactg gtcatcttat tttcagggac catgattatc
    37081 cctgggctgg tagcactcat ctaccgggat ggagcgggcc gcgcttttac ccagaccttt
    37141 tttgtcgccc tcgccattgg ctctatgctt tggtggccga accgcaaaga gaagggcgaa
    37201 ctgaaatccc gtgaggggtt tctgatagtg gtgctgttct ggaccgtgct gggtagcgtc
    37261 ggtgcgctcc cttttatctt ctcggaaagc ccgaacctca cgattaccga tgcgtttttt
    37321 gaatctttct ctggcctgac caccacgggg gccactacgc tggtggggct ggattcgctc
    37381 cctcatgcca tcctctttta tcgccagatg ctgcaatggt ttggcggtat ggggatcatc
    37441 gtgctggcgg ttgcgatact gcctatcctc ggcgtgggtg ggatgcagct ctatcgcgca
    37501 gaaatgcccg gcccgctgaa agataacaaa atgcgcccgc gaattgcgga aacggcgaaa
    37561 accctgtggt tgatttatgt cttgctgacc gtcgcctgtg cgctggcgtt gtggtttgct
    37621 ggaatggatg cctttgatgc catcggccat agctttgcga ccatcgctat tggcggcttc
    37681 tcgacacatg atgccagtat cggttatttc gacagcccga ctattaacac tatcattgct
    37741 atcttcctgc tgatctctgg ctgtaactac ggtctgcact tttcactgtt aagtgggcgt
    37801 agtctgaagg tttattggcg cgatccggaa tttcgcatgt ttatcggcgt acagttttca
    37861 ctggtggtta tttgtacact cgtactgtgg tttcataatg tctacagttc ggcgctgatg
    37921 acaattaacc aggcgttttt ccaggtggtg tcgatggcga caaccgccgg gtttacaact
    37981 gacagcattg cccgctggcc gctctttttg ccggtactgc ttttatgttc agcttttatc
    38041 ggcggttgcg ccgggtcaac gggcggtggc ctgaaagtga tccgcatcct gctgctgttt
    38101 aagcagggga accgtgagct gaaacgactg gtgcatccga acgccgtcta tagcattaag
    38161 ctggggaatc gcgcactgcc ggaacgtatc ctcgaagccg tttggggatt tttctccgcc
    38221 tatgcattgg tgtttattgt cagtatgctg gcgattatcg ccacgggcgt ggatgacttt
    38281 tctgcctttg cctcggttgt tgcaacattg aataacctgg ggccagggct tggcgtggtt
    38341 gctgataact ttaccagtat gaacccggtg gctaaatgga tcctgattgc caacatgctg
    38401 tttggtcgtc tcgaggtctt tacattgctg gtgctcttta ccccgacttt ctggcgtgaa
    38461 tgatggagta atacgtgaaa acattaattc ttttctcaac aagggacgga caaacgcgcg
    38521 agattgcctc ctacctggct tcggaactga aagaactggg gatccaggcg gatgtcgcca
    38581 atgtgcaccg cattgaagaa ccacaatggg aaaactatga ccgtgtggtt attggcgctt
    38641 ctattcgcta tggtcactac cattctgcgt tccaggaatt tgtcaaaaag catgcgacgc
    38701 ggttgaattc gatgccgagt gccttttact ccgtaaatct ggtggcgcgt aaaccggaga
    38761 agcgtactcc acagaccaac agctacgcgc ggaagtttct gatgaactcg caatggcgtc
    38821 ccgatcgctg tgccgtcatt gccggggcgc tgcgttaccc acgttatcgc tggtacgacc
    38881 gttttatgat caagctgatt atgaagatgt caggcggtga aacggatacg cgcaaagaag
    38941 ttgtctatac cgattgggag caggtggcga atttcgcccg agaaatcgcc catttaaccg
    39001 acaaaccgac gctgaaataa gcataaagaa taaaaa
//
LOCUS       DAZFJL010000025        38758 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFJL010000025 DAZFJL010000000
VERSION     DAZFJL010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.025"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            545..1633
                     /gene="pdeL"
                     /locus_tag="ACHJ8C_001326"
     CDS             545..1633
                     /gene="pdeL"
                     /locus_tag="ACHJ8C_001326"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HFO5551216.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGMIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(1675..2607)
                     /gene="yahB"
                     /locus_tag="ACHJ8C_001327"
     CDS             complement(1675..2607)
                     /gene="yahB"
                     /locus_tag="ACHJ8C_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5551217.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVHEAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(2699..3196)
                     /gene="yahC"
                     /locus_tag="ACHJ8C_001328"
     CDS             complement(2699..3196)
                     /gene="yahC"
                     /locus_tag="ACHJ8C_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO5551218.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLAPVSGISGVWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KSDGY"
     gene            3454..4059
                     /gene="yahD"
                     /locus_tag="ACHJ8C_001329"
     CDS             3454..4059
                     /gene="yahD"
                     /locus_tag="ACHJ8C_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO5551219.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKNCLALGVDINTCDRHGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLANTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            4099..4962
                     /gene="yahE"
                     /locus_tag="ACHJ8C_001330"
     CDS             4099..4962
                     /gene="yahE"
                     /locus_tag="ACHJ8C_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO5551220.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFNGLFRHGDKVQFDDQGITIGQHLHIEMSHCQRWLSPTLQMTAL
                     NFHLIAWQQWHDIIHQHLGENETLFNYRDDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     AVAVASMMGLGIGLTPSADDYLTGLALILFLPGHPAEKYKEEFYRGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            4952..6499
                     /gene="fdrA"
                     /locus_tag="ACHJ8C_001331"
     CDS             4952..6499
                     /gene="fdrA"
                     /locus_tag="ACHJ8C_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO5551221.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELGQAKNGDLMIVINGKSGVDNEQLLAEIEELFNTKAQSGSHEARYA
                     TIASAKKYIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVACFLGRGETSVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETLFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     REAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            6499..7917
                     /gene="yahG"
                     /locus_tag="ACHJ8C_001332"
     CDS             6499..7917
                     /gene="yahG"
                     /locus_tag="ACHJ8C_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO5551222.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTAKTILHAG
                     PPVTWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLSENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            7943..8197
                     /locus_tag="ACHJ8C_001333"
                     /pseudo
     CDS             7943..8197
                     /locus_tag="ACHJ8C_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8247..9197
                     /gene="yahI"
                     /locus_tag="ACHJ8C_001334"
     CDS             8247..9197
                     /gene="yahI"
                     /locus_tag="ACHJ8C_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO5551223.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIYADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            9207..10589
                     /gene="yahJ"
                     /locus_tag="ACHJ8C_001335"
     CDS             9207..10589
                     /gene="yahJ"
                     /locus_tag="ACHJ8C_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO5551224.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTIHCDAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTAHQTVEIQNGKIVALRENKQHPNAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVQVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            10805..10879
                     /locus_tag="ACHJ8C_001336"
                     /pseudo
     CDS             10805..10879
                     /locus_tag="ACHJ8C_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10966..12015
                     /gene="yahK"
                     /locus_tag="ACHJ8C_001337"
     CDS             10966..12015
                     /gene="yahK"
                     /locus_tag="ACHJ8C_001337"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO5551225.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPHDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDYMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSESKREAAKALG
                     ADEVVNSRNADEMVAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTITLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADRINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            12637..12880
                     /locus_tag="ACHJ8C_001338"
                     /pseudo
     CDS             12637..12880
                     /locus_tag="ACHJ8C_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12897..13571)
                     /gene="yahN"
                     /locus_tag="ACHJ8C_001339"
     CDS             complement(12897..13571)
                     /gene="yahN"
                     /locus_tag="ACHJ8C_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO5551226.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSSMRRHSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLHAETPT
                     WARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASWVIGAIIGVFALRLIY
                     EGVTQR"
     gene            13718..13993
                     /gene="yahO"
                     /locus_tag="ACHJ8C_001340"
     CDS             13718..13993
                     /gene="yahO"
                     /locus_tag="ACHJ8C_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO5551227.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVASQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(14094..15680)
                     /gene="prpR"
                     /locus_tag="ACHJ8C_001341"
     CDS             complement(14094..15680)
                     /gene="prpR"
                     /locus_tag="ACHJ8C_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO5551228.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQSSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFSRHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMQQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFAAALRQGLQASETMLVHYDWPGNIRE
                     LRNMMERLALFLSVESTPDLTPQFLQLLLPELARESAKTPIPGLLTAQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            15919..16809
                     /gene="prpB"
                     /locus_tag="ACHJ8C_001342"
     CDS             15919..16809
                     /gene="prpB"
                     /locus_tag="ACHJ8C_001342"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO5551229.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRICAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAEAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARGQVK"
     gene            16969..18138
                     /gene="prpC"
                     /locus_tag="ACHJ8C_001343"
     CDS             16969..18138
                     /gene="prpC"
                     /locus_tag="ACHJ8C_001343"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO5551230.1"
                     /translation="MSDTTILQNCTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            18172..19623
                     /gene="prpD"
                     /locus_tag="ACHJ8C_001344"
     CDS             18172..19623
                     /gene="prpD"
                     /locus_tag="ACHJ8C_001344"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO5551231.1"
                     /translation="MTAQINNIRPEFDREIVDIVDYVMNYEISSRVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLEPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGTMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAIASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            19663..21549
                     /gene="prpE"
                     /locus_tag="ACHJ8C_001345"
     CDS             19663..21549
                     /gene="prpE"
                     /locus_tag="ACHJ8C_001345"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO5551232.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHLQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            21779..23038
                     /gene="codB"
                     /locus_tag="ACHJ8C_001346"
     CDS             21779..23038
                     /gene="codB"
                     /locus_tag="ACHJ8C_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO5551233.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGDLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            23028..24311
                     /gene="codA"
                     /locus_tag="ACHJ8C_001347"
     CDS             23028..24311
                     /gene="codA"
                     /locus_tag="ACHJ8C_001347"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO5551234.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLHDGKISAIDTQSGVMPVTE
                     NSLDAEQGLVLPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(24432..25042)
                     /gene="lacA"
                     /locus_tag="ACHJ8C_001348"
                     /pseudo
     CDS             complement(24432..25042)
                     /gene="lacA"
                     /locus_tag="ACHJ8C_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
     gene            complement(25108..26361)
                     /gene="lacY"
                     /locus_tag="ACHJ8C_001349"
     CDS             complement(25108..26361)
                     /gene="lacY"
                     /locus_tag="ACHJ8C_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO5551235.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(26413..29487)
                     /gene="lacZ"
                     /locus_tag="ACHJ8C_001350"
     CDS             complement(26413..29487)
                     /gene="lacZ"
                     /locus_tag="ACHJ8C_001350"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO5551236.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEKAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFQVTTLFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPELWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEMRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDANGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRNIEVTSEYLFRHSDNELLHWSVALDGKPLASGEVPLDVAPQDKQVIE
                     LPELPQPESTGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHAIPQ
                     LTTSGTDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGHGEMVINVDVAVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGHYHYQLVWCQK"
     gene            complement(29610..30692)
                     /gene="lacI"
                     /locus_tag="ACHJ8C_001351"
     CDS             complement(29610..30692)
                     /gene="lacI"
                     /locus_tag="ACHJ8C_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO5551237.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQACHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPITEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(30729..30827)
                     /locus_tag="ACHJ8C_001352"
                     /pseudo
     CDS             complement(30729..30827)
                     /locus_tag="ACHJ8C_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
     gene            30894..31433
                     /gene="yaiL"
                     /locus_tag="ACHJ8C_001353"
     CDS             30894..31433
                     /gene="yaiL"
                     /locus_tag="ACHJ8C_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO5551238.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKITQTQLINGRLAIARLLVDNNNEGEYVIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(31559..32392)
                     /gene="fghA"
                     /locus_tag="ACHJ8C_001354"
     CDS             complement(31559..32392)
                     /gene="fghA"
                     /locus_tag="ACHJ8C_001354"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO5551239.1"
                     /translation="MELIEKYASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAENEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPNLVMHHFPATARKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(32485..33594)
                     /gene="frmA"
                     /locus_tag="ACHJ8C_001355"
     CDS             complement(32485..33594)
                     /gene="frmA"
                     /locus_tag="ACHJ8C_001355"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO5551240.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFELARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(33629..33904)
                     /gene="frmR"
                     /locus_tag="ACHJ8C_001356"
     CDS             complement(33629..33904)
                     /gene="frmR"
                     /locus_tag="ACHJ8C_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO5551241.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            34210..35172
                     /gene="tauA"
                     /locus_tag="ACHJ8C_001357"
     CDS             34210..35172
                     /gene="tauA"
                     /locus_tag="ACHJ8C_001357"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5551242.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVATSQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDIPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            35185..35952
                     /gene="tauB"
                     /locus_tag="ACHJ8C_001358"
     CDS             35185..35952
                     /gene="tauB"
                     /locus_tag="ACHJ8C_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO5551243.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQYGSIQLAGKGIQGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGVEK
                     MQRLEIAHQMVKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQIQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            35949..36776
                     /gene="tauC"
                     /locus_tag="ACHJ8C_001359"
     CDS             35949..36776
                     /gene="tauC"
                     /locus_tag="ACHJ8C_001359"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO5551244.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTLSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRILLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAALSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            36773..37624
                     /gene="tauD"
                     /locus_tag="ACHJ8C_001360"
     CDS             36773..37624
                     /gene="tauD"
                     /locus_tag="ACHJ8C_001360"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO5551245.1"
                     /translation="MSERLSITPLGPYIGAQITGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIQTPPAGAILAAKELPSTGGDTLWASGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEAEHQRWREAVAKNPPLLHPVVRTHPVTGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQGRIMHRATILGD
                     IPFYRAG"
     gene            complement(37664..38638)
                     /gene="hemB"
                     /locus_tag="ACHJ8C_001361"
     CDS             complement(37664..38638)
                     /gene="hemB"
                     /locus_tag="ACHJ8C_001361"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO5551246.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         9437 a         9908 c        10401 g         9012 t
ORIGIN      
        1 aaatattgtt tttattgtca ttgttataat gttggttatc tattgctgaa tggattcagt
       61 cttaaaaagc aggttgttaa gggataggca tagagtaatg acgtgtatgc ataaccaaca
      121 tctttactca ttatgtcatt gaatgttgac gctatgtgtt tatgaggttg aggtattttc
      181 agttgatctg gattgttaaa ttcatataat gcgcctttac tcatgaatgg gcgtcaatac
      241 gtagtgggaa attataaata tcgaaatagt ccaactactt ctttattacc aaaaatgagt
      301 atctgaattt taatattgca ttcttgcgtg attatctcct gagtttgact tgtgattacc
      361 tttttaaggt atttagcgta actgtttttg agcgagcatc agaggtaaag ataatcttct
      421 tgatagtgat gtgggatgtt atacgtatgg catcgctaat gtttatggtt accccttatg
      481 tgtgctcagg aatccacagg taatcactca tactgaacag cgataaaaga taaaaggtgt
      541 gttcatgaat tcatgtgatt ttcgtgtttt tctgcaagag ttcggtacaa cggttcattt
      601 gtcattgcct ggtagcgtat ccgagaaaga acgactgcta ctcaagctgc tgatgcaggg
      661 aatgtctgta acagaaatat cacagtacag aaatcgcagt gcaaagacca tttcacatca
      721 aaagaaacag ctctttgaga aactggggat tcagagcgat attactttct ggcgggatat
      781 tttctttcag tacaatccgg agatcatatc cgccacgggt aataatagtc acaaatatat
      841 taatgataat cactatcacc atatcgtcac gcctgaagcc atcagtctgg cgttggaaaa
      901 ccacgagttt aaaccgtgga tccaaccggt tttctgcgcg cagactggag tactgacggg
      961 ctgtgaagtg cttgtccgct gggaacatcc acaaacggga atgatcccac cggatcagtt
     1021 tattcctctg gcggagtcat ccggtcttat tgtcataatg actcgccagc tgatgaaaca
     1081 gactgcggat attctgatgc cggtaaaaca tttgctgccg gacaatttcc atattggtat
     1141 caacgtctcg gcgggctgtt ttttggctgc gggatttgaa aaagagtgtc tgaacctggt
     1201 taagaaatta ggtaacgata aaatcaagct ggttcttgag ctgacggaac gtaatcctat
     1261 tccggtaacg ccagaagcca gagcgatatt tgacagcctt catcagcaca atattacttt
     1321 tgcgctggat gactttggta cgggttatgc gacctatcgt tacttgcagg cgttcccggt
     1381 cgattttatt aaaattgaca agtcgtttgt gcaaatggcg agtgtcgatg aaatctccgg
     1441 tcatattgtg gacaatattg tcgaactggc gcgtaagcct ggtctgagta tcgtggcgga
     1501 aggggtagaa acccaggagc aggcggattt aatgatcggt aaaggcgttc actttttaca
     1561 gggctatttg tactctccgc cagtaccggg taataaattt atctctgaat gggtaatgaa
     1621 agctggtggt tgatgtaaac cgctattcac agcgcatcgg gaggttggca gcgattaatt
     1681 ctcccgatgc agtaaatcct gatagatccc cgttaatatg ccatttggcg caaattgttt
     1741 tttaatccac tgcgtcacct gaccggtggc agagtgctgg gttgcgagta acatgcgtga
     1801 atcctggcgc gggttatgga tttgtcgggt aaccagcaag gattgcgcca tcgcctcatg
     1861 gaccatgtaa tccggcaaaa aaccaattcc ttcacctaaa atctggcact gacatttggt
     1921 gttgaaatcg ggcaccagaa tcgattcctg cccatgcagc aaccagccta cttttttatt
     1981 aatcgtatgc gcggtgtcct ccaccatgat attagggtag agacgcagct ggctttcggc
     2041 gatcggctcc ggcacaaagg ctaatgggtg atccggggcg atggcaaaag cccagcgaat
     2101 cgcgccaatc tccgtgtaat caatgccgcc accgtccagc agcgtgtcgg gtgcgccgat
     2161 agcgatattt gcttgattat taataatcgc atcccagacg ccgttataca cttcggtggt
     2221 gacggtaatc tggcaggtag gaaactgctt tttcagcacc tgtaataagc gtgcggtgtg
     2281 tttgggcgta taaagcagct gattaataca aatgcgcaca cgcgcttcta tgccctgcga
     2341 aatagtatcg atactgcgtt taatggcatg aaaatcattc agcaggtcgg tagctttgcg
     2401 gaaaaaataa cgcccggact ccgttaactc aatgctgcgg gtactgcgag tgaacagcac
     2461 cacatccagc cccgtctcca tacgtttaat ggtgtagcta atggcggaag tggttaaacc
     2521 caactcttct gccgctttac tgaagctgcc aaaacgcgca gcggtagtaa atgccaacag
     2581 attctcttcg gtaaaaattg agttcattga cccgctcccg ccattttcga ttctgaattt
     2641 ctccagccat gactgtagag gtatccatta aagatggata atcatttttg aataattttt
     2701 aatacccgtc gcttttggtc gtcgcttcgc ttaatttttg tccgccgtat tctgaaatta
     2761 tccccacgca gttacccgcc agaaaaccca gtaatgtcac cgtccagttc atcccgctgg
     2821 caaaaaatag cgtcatgccg agaaagcccc cgggaataaa agacagtaac cagaaacgcc
     2881 cttgccagac caccaccgcc gagaagggta cagtcgtaat tacgctcgcc cagaccccgg
     2941 aaatcccact caccggggcc agccaaccgg cagccagcgc ggcgaaaaac gcccacacca
     3001 tcccggaata attaaccgcc aggcttctga taaaccccga tttaccgccg cctgcggcat
     3061 aaaaactgca aaaggcgacg aagccaatgg ttcctaataa ttcccatccc tgagagagcc
     3121 cgacatgtga agacactaac tgccaaagcc ctgcacaaat accgacggtg acacccgttg
     3181 ccgtgagtcc gttcatcttg ttcccctctg gttagacatg gacgaaagct actcttacat
     3241 gtgcagacta tagagaaata aaacctgatt gagtgaaagg gtatcgggtc aaagaaacaa
     3301 atattgcact accgcacacg gcgaaaagat tgttgaacat cattcaacaa aaaggcgaaa
     3361 tacggcgaat taaatttaag cgggttcttt tatcagatta tcgttgctat tctcaattca
     3421 tcgaaaacag ccacaaaaac caggtgaatg tgaatgacta taaaaaatct acctgcagac
     3481 tatttattgg ctgcgcaaca gggtgatatc gataaagtaa aaaactgcct tgcgctgggt
     3541 gtcgatatta atacctgcga tcgccacggg aaaacggcaa ttacgctggc aagtttatat
     3601 cagcaatatg cttgcgttca ggcattaatt gatgccggag cggatattaa taaacaagat
     3661 catacctgtt taaatccttt tttaattagc tgtctgaacg atgatttaac gctactacga
     3721 attattttac cggctaaacc cgatcttaat tgcgtaaccc gttttggcgg tgtcggcctg
     3781 acgcctgcct gtgaaaaagg ccatttaagt attgtaaaag agcttttggc aaatacggag
     3841 attaacgtta accagactaa ccatgttggc tggacaccgt tgctggaagc gattgtgctt
     3901 aacgatggcg ggattaaaca gcaggcgatt gtccagttat tgctggaaca cggtgccagc
     3961 ccgcatctga ccgataaata tggcaaaacg ccgctggaac tggcgcggga acggggcttt
     4021 gaagagattg cgcagttgct gatcgccgca ggtgcataaa ccgggaggct tgttatcaac
     4081 acaccagaaa aacggtgtgt gtgggcgcta actgcggatg ctgattttct ggcgcagcgg
     4141 gggcaaggac aggttgaaca ggtctttgcc agagcggtaa atatcgcact cccggctcgc
     4201 cagcagttgc tgacgctgct ttgtgaagag tacgacaatg cgcctaacag ttgtcggttg
     4261 gcactcactc actttaatgg tctgttccgg catggtgata aggttcagtt tgacgatcaa
     4321 ggcattacga ttggtcaaca tcttcatata gagatgagtc attgccagcg ttggctgtcc
     4381 ccaaccttgc aaatgaccgc tctgaatttt caccttatcg cctggcaaca gtggcacgac
     4441 attattcatc agcacctggg ggaaaatgaa accctgttta attatcgcga cgataatccg
     4501 ttttatcagg cgttaaataa agaactacat attaaacgac gggcagttat tcaggccgta
     4561 aacgataaac aaaatatcgc cgtagcggtc gccagtatga tgggcttagg gattggcctt
     4621 acgccatcag ccgatgatta tttaacaggt ctggcgctta ttttatttct tcccggacat
     4681 ccggcggaaa aatacaaaga ggaattttat cgcggtctgc aacgcggcag aaataatacc
     4741 acgttattaa gtgccatcac gctggaagcc gcattacaac aacgctgccg ggaaaatatt
     4801 catcgtttta ttcacaacat tatttatgac atccctggga acgcaactca ggcaatagaa
     4861 aaaattaaac atattggctc cagttccggc tgcgacatgc tgtatggcat ggccgatggt
     4921 tgtgcgctga gtcaaaccta cggagggaat tatgtcagtt aaaatagtca ttaaaccgaa
     4981 tacctatttt gattctgtct cgctgatgtc tatctccacg cgtgcaaata aactcgacgg
     5041 cgtcgagcag gcatttgtgg cgatggcgac cgaaatgaac aaaggcgtgt tgaagaattt
     5101 aggactgctg acgccggagc tggggcaggc gaaaaacggc gacctgatga ttgtcatcaa
     5161 cggtaaatcg ggtgtggaca acgagcagtt gctggcggag attgaagaac tgttcaacac
     5221 caaagcgcaa agcggctcgc acgaggcgcg ttacgccact attgccagcg ccaaaaagta
     5281 tatcccggaa agtaacctgg cagtgatttc ggtcaacggt ctgtttgccg ctcgcgaagc
     5341 gcgtcaggcg ctgcaaaatg acctcaacgt gatgctgttt tccgataatg tctcagttga
     5401 agatgaactg gcgctcaaac agttggccca tgaaaaaggg ctgctgatga tggggccaga
     5461 ctgcggcacg gcgattatca acggcgcggc gctctgcttt ggtaacgccg tgcgtcgcgg
     5521 caacatcggt attgttggcg catctggcac cggcagccag gaattgagcg tccgtatcca
     5581 tgaatttggt ggcggcgttt cacaactgat cggcaccggc gggcgcgacc tgagcgagaa
     5641 aatcggtggc ctgatgatgc tcgatgccat cgggatgctg gaaaacgatc cgcaaactga
     5701 aatcattgtg cttatctcca aaccgcctgc gcctgcggtg gctcgcaaag tgctggaacg
     5761 cgcacgcgcc tgccgcaagc cagtggtggc ctgcttcctc ggtcgtggcg aaacgtcagt
     5821 ggatgagcag gggctacagt ttgctcgcgg cagcaaagag gcggcattga aagcggtgat
     5881 gctctccggc gtgaaacaag aacatctcga cctgcatacg cttaaccagc cgttgattgc
     5941 ggatgtgcgg gcgcgtctgc aaccgcagca gaaatacatt cgtggtctgt tctgcggcgg
     6001 tacgctgtgt gacgaaaccc tgttcgcggt gatggaaaaa catggcgatg tctacagcaa
     6061 tatccagccc gatcctgaat tccgcctgca agatatcaac cgcagcatca aacacacctt
     6121 cctcgacttt ggcgatgatg acttcaccaa cggcaagccg catccaatga ttgacccgac
     6181 caaccgtatc agccgcttga tcgaagaagc gcgcgatcca gaagtggcgg tgatcgtgat
     6241 ggattttgtg ctcggatttg gatcgcatga agatccggtc ggttccacca tcgaggcgat
     6301 cagagaagcg aaagcgatcg ccgctgccga aggacgcgag ttgatcattc tcgcctatgt
     6361 gctgggcacc gatctcgata cgccatcatt agaacaacaa agccagatgc tgcttgatgc
     6421 cggagtgatt ctggcgagca gcagcaccaa taccggattg ctggcgcgtg aatttatctg
     6481 caaaggggag gaagcctgat gagccagtca ctgtttagcc aaccgttgaa cgttattaac
     6541 gtcggtatcg ccatgtttag cgatgatctg aaaaagcagc atgtagaagt gacccaactc
     6601 gactggacgc cgccggggca gggcaatatg caggtggtgc aggcgctgga taacattgcc
     6661 gattcgccgc tggcagacaa aatcgccgcc gctaaccagc aggcgctgga gcgcattatc
     6721 cagtcgcatc cggtgctaat cggctttgat caggcgatta acgtggtgcc gggcatgacg
     6781 gcgaaaacca ttcttcacgc cgggccaccg gtcacctggg aaaaaatgtg cggcgcgatg
     6841 aaaggcgcgg tcaccggagc gctggtgttt gaaggactgg cgaaagatct cgacgaggcg
     6901 gctgagctgg cggcttccgg ggagatcacc ttctcgccgt gccacgaaca cgactgtgtg
     6961 ggatcgatgg cgggcgtcac ctcggcctcg atgtttatgc acatcgtgaa aaacaaaacc
     7021 tatggcaaca tcgcttatac caacatgagc gagcagatgg cgaagatttt gcgtatgggc
     7081 gctaacgacc agagcgtgat cgaccgcctc aactggatgc gcgatgtgca gggacccatg
     7141 ctgcgcgacg cgatgaaaat tatcggcgaa atcgatctgc gcttaatgct ggcgcaggcg
     7201 ctgcatatgg gcgatgaatg ccataaccgc aataacgccg ggacgacgct gctgattcag
     7261 gcgctgacgc cggggattat tcaggcaggt tattccgtcg agcaacagcg cgaagtgttt
     7321 gagtttgtcg ccagcagcga ctacttctcc ggcccgacgt ggatggcgat gtgtaaagcg
     7381 gcgatggatg cggcgcacgg catcgaatac agcaccgtgg tgaccaccat ggcgcgtaac
     7441 ggcgtcgagt tcggcctgcg ggtcagcggc ctgccggggc agtggtttac cggcccggcg
     7501 cagcaggtca tcggcccgat gtttgccggt tataagccgg aagattcagg gctggatatt
     7561 ggcgacagcg ccatcaccga aacctacggt atcggcggat ttgcgatggc gaccgcgccc
     7621 gcgatcgtcg cgctggtggg tggcacggtg gaagaggcta tcgatttctc ccgtcagatg
     7681 cgtgaaatca cccttagcga aaaccccaac gtcaccattc cgctgctcgg ttttatgggc
     7741 gttccgtcgg caatcgacat cacccgcgtg ggtagcagcg gcattctgcc agtgatcaac
     7801 accgccatcg cccataaaga tgcgggcgtc ggcatgattg gcgcgggcat tgtgcatcca
     7861 cccttcgcct gcttcgagaa agccattctc ggctggtgcg aacgttacgg cgtctgagtt
     7921 acatgtttat gccggatgcg acatgaatgt cttatccggc ctacgaatgg cgcggaaatc
     7981 tgtaggcctg ataagcgtgg cgattcaggc attggccacc acagccggat gcggcgtgaa
     8041 cgccttatcc ggcctacgag tggcgcaaga atctgtaggc ctgataagcg tagcgcatca
     8101 ggcattggtc accacagccg gatgcggcgt gaacgcctta tccggcctac gagtggcgca
     8161 agaatctgta ggcctgataa gcgtagcgca tcaggcagtc tggcgttggt cataacccca
     8221 tcaccctctg tcgcggacat aacaacatga aagaacttgt ggtcgttgcc attggtggca
     8281 acagcattat caaagataac gccagccagt cgattgagca tcaggcggag gcggtgaaag
     8341 ccgtggccga tacggtgctg gaaatgctgg cttccgatta cgacattgtg ctgacccacg
     8401 gcaacgggcc gcaggtcggg ctggatttac gccgcgcgga gattgcccac gagcgcgaag
     8461 ggctgccctt aacgccgctg gcgaactgtg tggcggatac gcaaggcggc attggctact
     8521 tgatccaaca ggcgctgaat aaccgactgg cgcgtcacgg tgagaaaaaa gccgtcaccg
     8581 tggtgactca ggtggaagtg gataaaaacg atccggggtt tgcccatccc accaagccca
     8641 tcggcgcatt ctttagtgaa agccagcgtg acgaattaca aaaggcaaac cctgactggc
     8701 gttttgttga agatgccgga cggggctatc gccgcgtggt cgcctcgccg gaaccgaaac
     8761 gtattgtcga agcacctgcc attaaggcgc tgatccaaca aggctttgtg gtgattggcg
     8821 cgggcggcgg tggaattccg gtggtgcgca ctgaagcggg ggattaccaa agcgtggacg
     8881 cggttatcga caaagatctc tctaccgcgc tactggctcg cgaaatttac gccgacattc
     8941 tggtgatcac cacgggcgtg gaaaaagtgt gtattcattt tggtaaaccg cagcagcagg
     9001 cgctggatcg ggtggatatt gccaccatga cccgctatat gcaggaaggg catttcccac
     9061 ccggcagcat gttgccaaaa atcatcgcca gcctgacgtt cctggagcag ggcggtaaag
     9121 aagtgattat caccacgccg gaatgcctgc ctgcggcgct gcgtggtgaa acgggcaccc
     9181 atattattaa aacgtaagga cgtaagatga aagaaaacaa tagccgccgt gagtttctga
     9241 gccagagcgg taagatggtc accgccgccg cgctgtttgg tacctctgtg ccgctcgccc
     9301 atgcggcagt ctctggcaca atccattgcg acgcgaataa caccatgaaa atcactgacc
     9361 cgcactacta tctcgacaac gtgctgctgg aaaccggttt tgactacgaa aacggtgtgg
     9421 cagtacagac ccgcacggcg caccagaccg tagagattca gaacggcaaa attgttgccc
     9481 tgcgcgagaa caagcagcat ccaaacgcca cgctgccgca ctatgacgct ggcggtaagc
     9541 tgatgctgcc caccacccgc gacatgcata ttcatctcga caaaaccttc tacggcggac
     9601 cgtggcgctc gctcaatcgt ccggcaggca ccaccatcca ggacatgatc aaactcgagc
     9661 agaaaatgct gccggaactg caaccgtaca cgcaggaacg ggcggaaaaa ctgatcgatt
     9721 tattgcagtc gaaaggcacc accattgccc gcagccattg caatatcgaa ccggtttccg
     9781 gcctgaaaaa tctgcaaaat ttgcaggcgg tgctggcgcg acgtcaggcg ggcttcgagt
     9841 gtgaaattgt cgccttcccg cagcacggtt tgctgctgtc gaaatcggaa cccttaatgc
     9901 gtgaagcaat gcaggcgggt gcgcattacg tcggcgggct ggacccgacc agtgtcgatg
     9961 gcgcgatgga aaaatccctc gacaccatgt tccagattgc gctggactac gacaaaggcg
    10021 tcgatatcca cctgcacgaa accactccgt cgggcgtggc agccatcaat tatatggttg
    10081 aaacggtaga gaaaacgcca cagctgaagg gcaagctgac catcagtcac gcctttgcgt
    10141 tggcaacgct caacgaacaa caggtagatg aactggcgca ccggatggcg gcgcagcaaa
    10201 tttctatcgc ctcgacggtg ccgattggca cgctgcatat gccgctcaaa cagttgcacg
    10261 acaaaggcgt acaagtcatg accggcaccg acagcgttat cgaccactgg tctccctacg
    10321 gcctgggcga catgctggaa aaagccaatc tctacgcgca gctctatatt cgtcctaacg
    10381 aacagaattt gtcccgttcg ctgtttttag ccactggcga tgtattgccg ctcaacgaaa
    10441 aaggcgagcg cgtgtggccc aaagcgcagg atgacgccag ctttgtgctg gtggacgcct
    10501 cctgttccgc cgaggcggtg gcgcgtattt cgccgagaac ggcgacgttc cataaagggc
    10561 aactggtgtg gggaagtgtg gcaggttgat atgcatttaa tatgtctgca acggtgaaca
    10621 gtaagagatt taagccccag ggagtggggc ggcctgagta tgagtgtgtt ggcagaaatg
    10681 gacattatct ttgaagaata tgcccttatt gacgttgtgt agataactat ttgtctacgt
    10741 gaaaatatca tcagttttgc cgatttttgt cttttacgga tagcaaatat cacacttata
    10801 ggcgctcgcc ttatggggaa cgttgctgct ctgtttcatt caggctcgcg gcatgttggg
    10861 gctggatcgg gtggtttaaa tattggctat attcaatgga cgcgttttgc cgcgatgaca
    10921 tatcaggcat cgccaaatac acatagctaa tcaggagtaa acacaatgaa gatcaaagct
    10981 gttggtgcat attccgctaa acaaccgctt gaaccgatgg atatcacccg gcgtgaaccg
    11041 ggaccgcatg atgtcaaaat cgaaatcgct tactgtggcg tctgccattc cgatctccac
    11101 caggtccgtt cggagtgggc ggggacggtt tacccctgcg tgccgggtca tgaaattgtg
    11161 gggcgcgtgg tagccgttgg tgatcaggta gaaaaacatg cgccgggcga tctggtcggt
    11221 gtcggctgca ttgtcgacag ttgtaaacat tgcgaagagt gtgaagacgg gctggaaaac
    11281 tactgtgatt acatgaccgg cacctataac tcgccgacac cggatgaacc gggccatact
    11341 ctgggcggct actcacaaca gatcgtcgtt catgagcgat atgttctgcg tattcgtcac
    11401 ccgcaagagc agttggcggc ggtggctcct ttattgtgtg caggaatcac cacgtactcg
    11461 ccgctacgcc actggcaggc cgggccgggt aaaaaagtgg gcgtggtcgg catcggcggt
    11521 ctgggacata tggggattaa actggcccac gcgatggggg cgcatgtggt ggcatttacc
    11581 acttctgagt caaaacgcga agcggcaaaa gccctggggg ccgatgaagt tgttaactca
    11641 cgcaatgccg atgagatggt ggctcatgtg aagagtttcg atttcatttt gaatacagta
    11701 gctgcgccac ataatctcga cgattttacc accttgctga agcgcgatgg caccataacg
    11761 ctggttggtg cgcctgcgac accgcataaa tcgccggaag ttttcaacct gatcatgaaa
    11821 cgccgcgcga tagctggttc tatgattggc ggcattccag aaactcagga gatgctcgat
    11881 ttttgcgccg aacatggcat cgtggctgat atagagatga ttcgggccga tcgaattaat
    11941 gaagcctatg agcgaatgct gcgtggtgat gtgaaatatc gttttgttat cgataatcgc
    12001 acactaacag actgaaaaaa ttaataaaca tcctgtggtt taacatatta acttcgctcc
    12061 acttaacttt ttagttaagg agagcgaaat aatatcaaag tagtggtaaa cctatagcgt
    12121 aaattcagat tgttaaatta actccactac tttacacgtt agttatgagc gatttctgat
    12181 agtgtctggt ttaatcagag ctttattatc tgcgacgttt atttttattt aagagagcca
    12241 cctgcaagtt gttattccac cagagagtga atcgcaaagc aggtcatcat ttcatctgta
    12301 tatctgtatg ccaaatggca ttccccctcc ttttccgccg atattttgtt gtgaacggcg
    12361 gagtttcgct taaaaaagca ccagcagtgg ttttacagct atatggtgta taaaaaatgt
    12421 tctcatacag atgttttgtg aatgtgtggg ttgacattca tatgaaaaaa atcataattc
    12481 catcatgttt gtgtggggtc ttttctgtat cttacgcatc gcactcaagc ctgacagaaa
    12541 atatgttgta aggctcatat caaaaaccgc cattagctca tcaggaagag cagacgacaa
    12601 ccaggattgt tgtatggtac ggggttcgag gcctcgatgg cggtccaact tttcaaaact
    12661 tttctgaatc agatcccctt attatcatcc ctgcaaagtg gaacattgcc attgtttggc
    12721 tattcaggac gggtcagacc aatgaaaaag cccacacagg ggagagtggc tgaaatggga
    12781 agctaaagac tcaagtaaac ttatcggaaa taaggaccac gcattacggg ggctatcatc
    12841 gccgatggtt gggattcggc aaatcaggtt gatgacgtag agagtaaatt atctgctcac
    12901 cgctgcgtca ccccttcgta aatcaggcgt agcgcgaata caccaataat tgcaccaata
    12961 acccaactgg caacgcgttg catacgccca taagcacgac gcactgcggg caaagaaaac
    13021 gcctgactaa gaaagactcg ccagataatt gatgcgagca caatccccgc ccaggccatt
    13081 aatcgtgccc atgttggtgt ttcggcatgt aatgttactg agaaaatact gataaaaaat
    13141 aaaacggttt gcgggttaga gagatcggta attaatccac ggcggaaaaa gacataccag
    13201 ggggcgctaa tcggttgttg tagtgtgctc atttgcggtg ttgagtggcg gcgcatgcta
    13261 ctacaccacg caaaccataa aagataagca ccgccgacga ttctgataag cgaaaaaatc
    13321 tcctcacact gcgtaattag cgttgcaaga ccaaacaaac ccaacccgga ataaaatgca
    13381 tcgcccagcg ccacgcccag cccggtcaac acccctgcgc gtcgaccgga agccaggctg
    13441 gtttgtacta ccacaaagag attggctccc ggattaaaaa aagtaatcac gaacaatcct
    13501 acggtcaggt aaacggcgtg caaaggatcc atagtgattt catccataaa taagtgaagt
    13561 aacttcatca tatttctacc aaaaataatc gggtgcgaga gagatcacaa agtgtcttat
    13621 ttccggttac tggcgtttat gccctgactg aactaattat taatcaaccc aataatgtgg
    13681 gtgggtgata gtgtgataac aactctggag ccgtaatatg aaaataatct ctaaaatgtt
    13741 agtcggtgcg ttagcgtttg ccgttaccaa tgtctatgcc gcagaattaa tgaccaaagc
    13801 ggaatttgaa aaggttgcat cgcaatatga aaaaataggt gatatttcaa ccagtaatga
    13861 aatgtcgacc gcagatgcga aagaagattt gatcaaaaaa gccgatgaaa aaggggctga
    13921 tgtgttggta ctgacctccg gtcaaactga caataagatc cacggcacgg caaatattta
    13981 taagaagaag taattctgaa tcctatgtaa acatccccga tgcgtaagtt tatcggtgat
    14041 catcatctat tgaaatttat gccggataaa gcgttcgcgc tgcattcggc agttcagctt
    14101 ttcagccgcc gccagaacgt cgtccggctg atacctaaat aattcgccgc ggctgtttta
    14161 tcgccattaa atttctccag tgcctgttgt gctgtcagca agcctggaat gggagtcttc
    14221 gccgactcgc gtgccagttc cggcagtagc agctgcaaaa attgcggcgt taaatccggc
    14281 gtcgattcca cacttaaaaa cagcgccagt cgttccatca tattgcgcag ttcacgaata
    14341 ttgcccggcc agtcgtagtg caccagcatg gtttcgcttg cctgtaaccc ctggcgtaat
    14401 gcggcagcaa acggggcgga gagcgccgcc agagacactt tcaaaaagct ttccgccagc
    14461 ggcagaatat ccgccacccg ctcgcgtagc ggcggcagtt gcaggcgtaa aatactcagc
    14521 cgataaaaca ggtcacggcg aaactgccct tgctgcatat cttcttccag attgcagtga
    14581 gtggcgctaa tgacccgcac atctaccgga acaggctgat gcccgccgac gcgggtgacc
    14641 tctttttctt ccagcacccg taacaggcga gtctgcaaag gtagcggcat ttcgcctatc
    14701 tcgtcaagaa acagcgtgcc accgtgggcg atttcaaaca gcccggcgcg tcctccgcgt
    14761 cgcgagccgg taaacgcccc ttcctcatag ccaaacagct ccgcttccag cagcgattcg
    14821 gcaatcgccc cgcagttgac ggcaacaaac ggatgtgact ttttgccctg tcgcgcatcg
    14881 tggcgggaaa aatattcccg atgaatcgcc tgggccgcca gctctttgcc cgtccccgtt
    14941 tccccttcaa tcaacaccgc tgcactggag cgggcataca gcaaaatagt ctgccgtacc
    15001 tgttccatct gtggtgattg accgagcata tcgcccagca cgtaacgagt acgcagggcg
    15061 ttacgggtag catcgtgagt gttatggcgt aacgacatgc gcgtcatatc cagcgcatcg
    15121 ctgaacgcct ggcgcacggt ggcggcggaa tagataaaaa ttccggtcat tccggcttct
    15181 tctgccagat cggtaatcag ccccgcgccg accaccgctt cggtgccgtt agcttttagc
    15241 tcgttaatct gcccgcgtgc gtcttcctcg gtaatgtagc tactttggtc gaggcgcaaa
    15301 ttaaaggttt tttgaaacgc caccagcgct ggaatggttt cctgataggt gacaacaccg
    15361 atagaagagg tgagttttcc ggcttttgcc agtgcctgta acacatcgta gccgctcggt
    15421 ttaatcaaaa taactggcac tgacaggcga cttttaagat atgcgccgtt agagccagca
    15481 gcgatgatgg cgtcgcaccg ttcgttggcc agtttcttgc ggatgtaggt caccgctttt
    15541 tcaaagccaa gctggatagg ggtgatattc gccaggtgat caaactcaag gctgatatcg
    15601 cgaaacagct cgaacaggcg cgttacagat accgtccaga taaccggttt gtcgtcattc
    15661 agccgtggtg gatgtgccat agcgcaccgc aaagttaaga aaccgaatat tgggtttagt
    15721 cttgtttcat aattgttgca atgaaacgca gtgaaacatt gcctgaaacg ttaactgaaa
    15781 cgcatatttg cggattactt catgacttta tttataacaa attgaaatta aacatttaat
    15841 tttattcagt caattgtggc acaccccttg cttcgtcttt atcaacgcaa ataacaagtt
    15901 gataacaaag gatgggctat gtctctacac tctccaggta aagcgtttcg cgctgcactg
    15961 actaaagaaa atccattgca gattgttggc actatcaacg ctaaccatgc gctgctggcg
    16021 cagcgtgccg gatatcaggc aatttatctc tccggcggtg gcgtggcggc agggtcgctg
    16081 gggctgcccg atctcggtat ttctactctt gatgacgtgc tgacggacat tcgccgtatc
    16141 accgacgttt gttcgctgcc gctgctggtg gatgcggata tcggttttgg ttcttcagcc
    16201 tttaacgtgg cgcgtaccgt gaaatcgatg attaaagccg gtgcggcagg attgcatatt
    16261 gaagatcagg ttggtgcgaa acgctgcggt catcgtccga ataaagcgat cgtctcgaaa
    16321 gaagagatgg tggatcggat ctgcgcggcg gtggatgcga aaaccgatcc tgattttgtg
    16381 atcatggcgc gcaccgatgc gctggcggta gaggggctgg atgcggcaat cgaacgagcg
    16441 gaggcctatg ttgaagcggg tgccgagatg ctgttcccgg aagcaattac cgaactcgcc
    16501 atgtaccgcc agtttgccga tgcggtgcag gtgccgatcc ttgccaacat tactgaattt
    16561 ggtgccacgc cgctgtttac caccgacgaa ttacgcagcg cccatgtcgc aatggcgctg
    16621 tatccccttt ctgctttccg cgccatgaac cgcgccgctg aacatgtcta caacgtcctg
    16681 cgccaggaag gcacgcagaa aagcgtcatc gacaccatgc aaacccgcaa cgagctgtac
    16741 gaaagcatca actactacca gtacgaagag aagctcgacg acctgtttgc ccgtggtcag
    16801 gtgaaataaa aacgcccgtt ggttgtattc gacaaccgat gcctgatgcg ccgctgacgc
    16861 gacttatcag gcctacgagg tgaactgaac tgtaggtcgg ataagacgca tagcgtcgca
    16921 tccgacaaca atctcgaccc tacaaatgat aacaatgacg aggacaatat gagcgacaca
    16981 acgatcctgc aaaactgtac ccatgtcatt aaaccgaaaa aatcggtggc actttccggc
    17041 gttccggcgg gcaatacggc gctctgcacc gtgggtaaaa gcggcaacga cctgcattac
    17101 cgtggctacg atattcttga tctggcggaa cattgtgaat ttgaagaagt ggcgcacctg
    17161 ctgatccacg gcaaactgcc aacccgtgac gaactcgccg cctacaaaac gaaactgaaa
    17221 gccctgcgtg gtttaccggc taacgtgcgt accgtgctgg aagccttacc ggcggcgtca
    17281 cacccgatgg atgttatgcg caccggcgtt tccgcgctcg gctgcacgct gccagaaaaa
    17341 gaggggcaca ccgtttctgg tgcgcgggat attgccgaca aactgctggc gtcacttagt
    17401 tcgattcttc tctactggta tcactacagc cacaacggcg aacgcatcca gccggaaact
    17461 gatgacgact ctatcggcgg tcacttcctg catctgctgc acggcgaaaa gccgtcgcaa
    17521 agctgggaaa aggcgatgca tatctcgctg gtgctgtacg ccgaacacga gtttaacgct
    17581 tccaccttta ccagccgggt gattgcgggc actggctctg atatgtattc cgccattatt
    17641 ggcgcgattg gcgcactgcg cgggccgaaa cacggcgggg cgaatgaagt gtcgctggag
    17701 atccagcaac gctacgaaac gccggacgaa gccgaagctg atatccgtaa gcgggtggaa
    17761 aacaaagaag tggtcattgg ttttgggcat ccggtttata ccatcgccga cccgcgccac
    17821 caggtgatca aacgcgtggc aaagcagctc tcgcaggaag gcggctcgct gaagatgtac
    17881 aacatcgccg atcgcctgga aacagtgatg tgggagagca aaaagatgtt ccccaatctc
    17941 gactggttct ccgctgtttc ctacaacatg atgggtgttc ccaccgagat gttcacacca
    18001 ctgtttgtta tcgcccgcgt cactggctgg gcggcgcaca ttatcgaaca acgtcaggac
    18061 aacaaaatta tccgtccttc cgccaattac gttggaccgg aagaccgcca gtttgtcgcg
    18121 ctggataagc gccagtaaac ctctacgaat aacaataagg aaacgtaccc aatgacagct
    18181 caaatcaaca acattcgccc ggaatttgat cgtgaaatcg ttgatatcgt cgattacgtg
    18241 atgaactacg aaatcagctc cagagtagcc tacgacaccg ctcattactg cctgctcgac
    18301 acgctcggct gcggtctgga agctctcgaa tatccggcct gtaaaaaact gctggagcca
    18361 attgtccccg gcaccgtcgt acccaatggc gtgcgcgtcc ccggaactca gtttcagctc
    18421 gaccccgtcc aggcggcatt taacatcggc acgatgatcc gttggctcga tttcaacgat
    18481 acctggctgg cggcggagtg ggggcatcct tctgacaacc tcggcggcat tctggcaacg
    18541 gcggactggc tttcgcgcaa cgcgatcgcc agcggcaaag cgccgttgac catgaaacag
    18601 gtgctgaccg gaatgatcaa agcccatgaa attcagggct gcatcgcgct ggaaaactcc
    18661 tttaaccgcg ttggtctcga ccacgttctg ttagtgaaag tggcttccac cgccgtggtc
    18721 gccgaaatgc tcggcctgac ccgcgaggaa attctcaacg ccgtttcact ggcgtgggtg
    18781 gacggccagt cgctgcgcac ctatcgccat gcgccgaaca ccggcacgcg taaatcctgg
    18841 gcggcgggcg atgctacttc ccgcgcggta cgtctggcac tgatggcgaa aaccggcgaa
    18901 atgggttatc cgtcagccct gactgcgcct gtgtggggct tctacgacgt ctcctttaaa
    18961 ggcgaatcgt tccgcttcca gcgcccgtat ggttcttacg tgatggaaaa tgtgctgttc
    19021 aaaatctcct tcccggcgga gttccactcc cagacggcag ttgaagcagc gatgacgctc
    19081 tatgaacaga tgcaggcagc aggcaaaacg gcggcggata tcgaaaaagt gaccattcgc
    19141 acccacgaag cctgtattcg catcatcgac aaaaaagggc cgctcaataa cccggctgac
    19201 cgcgaccact gcattcagta catggtggcg atcccgctgc tgttcgggcg cttaacggcg
    19261 gcagattacg aggacaacgt tgcgcaagat aaacgcatcg acgccctgcg cgagaagatc
    19321 aattgctttg aagatccggc gtttaccgct gactaccacg acccggaaaa acgcgccatc
    19381 gccaatgcca taacccttga gttcaccgac ggcacgcgct ttgaagaagt ggtggtggag
    19441 taccccattg gtcatgctcg ccgccgtcag gatggcattc cgaagctggt cgataaattc
    19501 aaaatcaatc tcgcgcgcca gttcccgact cgccagcagc agcgcattct ggaggtttct
    19561 ctcgacagaa ctcgcctgga acagatgccg gtcaatgagt atctcgacct gtacgtcatt
    19621 taagtaaacg gcggtaaggc gtaagttcaa caggagagca ttatgtcttt tagcgaattt
    19681 tatcagcgtt cgattaacga accggagcag ttctgggccg agcaggcccg gcgtattgac
    19741 tggcagacgc cctttacgca aacgctcgac cacagcaacc cgccgtttgc ccgttggttt
    19801 tgtgaaggcc gaaccaactt gtgtcacaac gctatcgatc gctggctgga gaaacagcca
    19861 gaggcgctgg cattgattgc cgtctcttcg gaaacagagg aagagcgtac ctttaccttc
    19921 cgccagttac atgacgaagt gaatgcggtg gcgtcaatgc tgcgctcact gggcgtgcag
    19981 cgtggcgatc gggtgctggt gtatatgccg atgattgccg aagcgcatat taccctgctg
    20041 gcctgcgcgc gcattggcgc tattcattcg gtggtgtttg ggggatttgc ttcgcacagc
    20101 gtggcggcgc gaattgatga cgctaaaccg gtgctgattg tctcggctga tgccggggcg
    20161 cgcggcggta aaatcattcc gtataaaaaa ttgctcgacg atgcgataag tcaggcacag
    20221 catcagccgc gtcacgtttt actggtggat cgcgggctgg cgaaaatggc gcgcgttagc
    20281 gggcgggatg tcgatttcgc gtcgttgcgc catcaacaca tcggcgcgcg ggtgccggtg
    20341 gcatggctgg aatccaacga aacctcctgc attctctaca cctccggcac gaccggcaaa
    20401 cctaaaggtg tgcagcgtga tgtcggcgga tatgcggtgg cgctggcgac ctcgatggac
    20461 accatttttg gcggcaaagc gggcggcgtg ttcttttgtg cttcggatat cggctgggtg
    20521 gtagggcatt cgtatatcgt ttacgcgccg ctgctggcgg ggatggcgac tatcgtttac
    20581 gaaggattgc cgacctggcc ggactgcggc gtgtggtgga aaattgtcga gaaatatcag
    20641 gttagccgca tgttctcagc gccgaccgcc attcgcgtgc tgaaaaaatt ccctaccgct
    20701 gaaattcgca aacacgatct ttcgtcgctg gaagtgctct atctggctgg agaaccgctg
    20761 gacgagccga ccgccagttg ggtgagcaat acgctggatg tgccggtcat cgacaactac
    20821 tggcagaccg aatccggctg gccgattatg gcgattgctc gcggtctgga tgacaggccg
    20881 acgcgtctgg gaagccccgg cgtgccgatg tatggctata acgtgcagtt gctcaatgaa
    20941 gtcaccggcg aaccgtgtgg cgtcaatgag aaagggatgc tggtagtgga ggggccattg
    21001 ccgccaggct gtattcagac catctggggc gacgacgacc gctttgtgaa gacgtactgg
    21061 tcgctgtttt cccgtccggt gtacgccact tttgactggg gcatccgcga tgctgacggt
    21121 tatcacttta ttctcgggcg cactgacgat gtgattaacg ttgccggaca tcggctgggt
    21181 acgcgtgaga ttgaagagag tatctccagt catccgggcg ttgccgaagt ggcggtggtt
    21241 ggggtgaaag atgcgctgaa agggcaggtg gcggtggcgt ttgtcatacc gaaagagagc
    21301 gacagtctgg aagaccgcga cgtggcacac ttgcaagaga aggcaattat ggcgctggta
    21361 gacagccaga ttggcaactt tggccgcccg gcgcacgtct ggtttgtctc gcaattgcca
    21421 aaaacgcgat ccggaaaaat gctgcgccgc acgatccagg cgatttgcga aggacgcgat
    21481 ccgggggatc tgacgaccat tgatgatccg gcgtcgttgg atcagatccg ccaggcgatg
    21541 gaagagtagg tcggataagg cgctcgcgcc gcatccgaca ccgtgcgcag atgcctgatg
    21601 cgacgctgac gcgtcttatc atgcctaccc actgttttta cacagataag tttcccccgc
    21661 ctttttgtac tcattcatat aagaaatata tccccatacg aaaacgattg ctttttatct
    21721 tcagatgaat agaatgcggc ggatttttgg gtttcaaaca gcaaaaaggg ggaatttcgt
    21781 gtcgcaagat aacaacttta gccaggggcc agtcccgcag tcggcgcgga aaggggtatt
    21841 ggcattgacg ttcgtcatgc tgggattaac cttcttttcc gccagtatgt ggaccggcgg
    21901 cactctcgga accggtctta gctatcatga tttcttcctc gcagttctca tcggtaatct
    21961 tctcctcggt atttacactt catttcttgg ttacattggc gcaaaaaccg gcctgaccac
    22021 ccatcttctt gctcgcttct cgtttggtgt taaaggctca tggttgcctt cactgctgct
    22081 gggcggcacc caggttggct ggtttggcgt tggcgtggcg atgtttgcta ttccggtggg
    22141 caaggcaacc gggctggata ttaatttgct gattgccgtc tccggtttac tgatgaccgt
    22201 caccgtcttc tttggcattt cggcgctgac agttctttcg gtgattgcgg ttccggctat
    22261 cgcctgtctt ggcggttatt ccgtgtggtt ggccgttaac ggcatgggcg acctggacgc
    22321 attaaaagcg gtcgttcccg cacaaccgtt agatttcaat gtcgcgctgg cgctggttgt
    22381 ggggtcattt atcagtgcgg gcacgctcac cgctgacttt gtccggtttg gtcgtaatgc
    22441 caaactggcg gtgctggtgg cgatggtagc ctttttcctc ggcaactcgt tgatgtttat
    22501 tttcggtgca gcgggcgcgg cggcactggg gatggcggat atctctgatg tgatgattgc
    22561 tcagggcctt ctgctgcctg cgattgtggt gctggggctg aatatctgga ccaccaacga
    22621 taacgcgctc tatgcgtcgg gtttaggttt cgccaacatt accggtatgt cgagcaaaac
    22681 cctttcggtg attaacggta ttatcggtac ggtctgcgca ttatggctgt ataacaattt
    22741 tgtcggctgg ttgaccttcc tttcggcagc tattcctccg gtgggcggcg tgatcatcgc
    22801 tgattatctg atgaaccgtc gccgctatga gcactttgcg accacgcgta tgatgagtgt
    22861 caattgggtg gcgattctgg ctgtcgcgct ggggattgcc gcaggccact ggttaccggg
    22921 aattgttccg gtcaacgcgg tattaggtgg cgcgctgagc tatctgatcc ttaacccgat
    22981 tttgaatcgt aaaacgacag cagcaatgac gcatgtggag gctaacagtg tcgaataatg
    23041 ctttacaaac aattattaac gcccggttac ccggcaaaga ggggctgtgg cagattcatc
    23101 ttcacgacgg aaaaatcagc gccattgata cgcaatctgg tgtgatgccc gtaactgaaa
    23161 acagcctgga tgccgaacaa ggtttagttt taccgccgtt tgtggagccg catattcacc
    23221 tggacaccac gcaaaccgcc ggacaaccga actggaatca gtccggcacg ctgtttgaag
    23281 gcattgaacg ctgggccgag cgcaaagcgt tattaaccca tgacgatgtg aaacaacgcg
    23341 cctggcaaac gctgaaatgg cagattgcca acggcattca gcatgtgcgt acccatgtcg
    23401 atgtctccga cgcaacgctg actgcgttga aagcgatgct ggaagtgaaa caggaagttg
    23461 cgccgtggat tgatctgcaa atcgtcgcct tcccgcagga agggattttg tcgtatccca
    23521 acggtgaagc gttgctggaa gaggcgttac gcttaggggc agacgttgtt ggggcgattc
    23581 cgcattttga atttacccgt gaatatggcg tggagtcgct gcataaaacc ttcgccctgg
    23641 cgcaaaaata cgaccgtctc atcgatgttc actgtgacga gattgatgat gagcaatcac
    23701 gctttgtcga aaccgttgct gccctggcgc accgtgaagg catgggcgcg cgagtcaccg
    23761 ccagccatac tacggcaatg cactcctata acggggcgta tacttcgcgc ctgtttcgtt
    23821 tgctgaaaat gtccggtatt aactttgtcg ccaacccgtt ggtcaatatt catctgcaag
    23881 gacgtttcga tacgtatcca aaacgtcgcg gcatcacgcg cgttaaagag atgctggagt
    23941 ccggcattaa cgtctgcttt ggtcacgatg atgtcttcga tccgtggtat ccgctgggaa
    24001 cggcgaatat gctgcaagtg ctgcatatgg ggctgcatgt ttgccagctg atgggctacg
    24061 ggcagattaa cgacggcctg aatttaatca cccaccacag cgccaggacg ttgaatttgc
    24121 aggattacgg cattgccgcc ggaaacagcg ccaacctgat tatcctgccg gctgaaaatg
    24181 ggtttgatgc gctgcgccgt caggttccgg tacgttattc ggtacgtggc ggcaaggtga
    24241 ttgccagcac acaaccggca caaaccaccg tatatctgga gcagccagaa gccatcgatt
    24301 acaaacggta atcggctggg ttaccgcgag cgtagtttat gccggatgcg gcgtgaacac
    24361 cttatccggc ctacgtagat ctctgaaatt gtaggcctga taagcggagc gcatcaggca
    24421 attttgctta tttaaactga agactcaact ttaaaatctt tgaaataata ttgcttatcc
    24481 cggtcgttta tttcgcgaat aacccgacaa ggaacgccag cagccacgac gtttggtgga
    24541 atgtcttttg tgacgacact acccgcgcca ataacagaat tatccccgat ggtgacgcct
    24601 ggattaataa ccacatgact tccgatccag acgttattgc caatcgttat cggaaaagag
    24661 tacatctctc cgttttttct caattcatgg tgtaccgggt gtcccgtaac ggaaagagta
    24721 acgttgggtg caatcagtac gttatcaccg atcgttaccg tgtagtcgtc gacaatggtt
    24781 aaattgaaat ttgcataaaa attgcggcct atatagatgt tggaaccgta agagaaatag
    24841 acgggggttc tacccaggcg ttttccccta ccgtggcaaa catttcttta atcagacttt
    24901 ctcttttatc aacttctgat ggatgcgagt gattaaactc atacattaac gtttttccac
    24961 gaagtctttt ttcaggtaag ccttcgcaca tatcggtaaa tagcttgcct gcttttattc
    25021 tttctgtcat cgacatgttc attgcgatca ttccgccctg atatgttggt cggataaggc
    25081 gctcgcgccg catccgacat tgattgctta agcgacttca ttcacctgac gacgcagcaa
    25141 ggaaagcggg ccggggccgc taagcgtgaa cacggaaatt aaggtgaagc ccagcgccac
    25201 cagacccagc accagataag cgccctggaa accgatgctt tcatacatat tgcccgccag
    25261 tacagacata aaaatcatcg ccagttgctt aaagaagcag aaacagacca gataaatcgt
    25321 cgctgaaaaa cgcacttcaa actggctggt aatatattta aagcagccca ccagcaggaa
    25381 cggtacttca aacatatgca gcgttttcag aataaccact tccagcgctg aggtggcgaa
    25441 cgatgagcca ataatacgta cagacataat agtgccagcc agcagcaggg catttttccc
    25501 accgatgcga ttaatgatca gtggcgcaaa gaacataatc gaggcattaa gtaattcgcc
    25561 cattgtcgtt acgtagccaa atacccgcgt accctgttca ccggtagcaa aaaacgaagt
    25621 aaagaaatta gcaaactgtt ggtcaaaaac atcgtaggtg caggaaacgc caataacata
    25681 cagtgacaaa aaccacagtt ttggctgtct gaacagttcc agcgccagct taaggctaaa
    25741 tgccgaatgg ttggcaccta ccgcattggc aaccgtggct gaagagggcg catccgtttt
    25801 ggcgaaaaag agtaaaacgg cgaggatgag tgcacagcca gaacccagcc agaaaacaaa
    25861 ctgattattg atggtgaaca tgatgccgac aatcgaggca cacagcgccc agccaacaca
    25921 gccaaacatc cgcgcgcgac caaattcgaa attactgcga cggctgactt tctcgataaa
    25981 tgcctctact gctggcgcac cggcgttaaa acaaaagcca agataaatac caccaacaat
    26041 cgatcctact aaaatgttgt attgtaacag tggtccgaag ataaaaataa agaacggcgc
    26101 aaacatcact aacatgccgg taataatcca cagcaggtat ttgcgcagcc cgagtttgtc
    26161 agaaagcaga ccaaacagcg gttggaataa tagcgagaac agagaaatag cggcaaaaat
    26221 aatacccgta tcacttttgc tgatatggtt gatgtcatgt agccaaatcg ggaaaaacgg
    26281 gaagtaggcc cccatgataa aaaagtaaaa gaaaaagaat aaaccgaaca tccaaaagtt
    26341 tgtgtttttt aaatagtaca taatggattt ccttacgcga aatacgggca gacatagcct
    26401 gcccggttat tattattttt gacaccagac caactggtaa tggtagtgac cggcgctcag
    26461 ctggaattcc gccgatactg acgggctcca ggagtcgtcg ccaccaatcc ccatatggaa
    26521 accgtcgata ttcagccatg tgccttcttc cgcgtgcagc agatggcgat ggctggtttc
    26581 catcagttgt tgttggctgt agcggctgat gttgaactgg aagtcgccgc gccactggtg
    26641 tgggccataa ttcaattcgc gcgtcccgca gcgcagaccg ttttcgctcg ggaagacgta
    26701 cggggtatac atgtctgaca atggcagatc ccagcggtca aaacaggctg cagtaaggcg
    26761 gtcgggatag ttttcttgcg gccccaggcc gagccagttt acccgctctg cgacctgcgc
    26821 cagctggcag gtcaggccaa tccgcgccgg atgcggtgta tcgcttgcca ccgcaacatc
    26881 cacattgatg accatctcac cgtgcccatc aatccggtag gttttccggc tgataaataa
    26941 ggttttcccc tgatgctgcc acgcgtgggc ggtcgtaatc agcaccgcgt cggcaagtgt
    27001 atctgccgtg cactgcaaca acgccgcttc ggcctggtaa tggcccgccg ccttccagcg
    27061 ttcgacccag gcgttagggt caatgcgggt cgcttcactt acgccaatgt cgttatccag
    27121 cggtgcgcgg gtgaactgat cgcgcagcgg ggtcagcagt tgtttttcat cgccaatcca
    27181 catctgtgaa agaaagcctg actggcggtt aaattgccaa cgcttattac ccagctcgat
    27241 gcaaaaatcc gttccgctgg tggtcagttg agggatggcg tgggacgcgg aggggagtgt
    27301 cacgctgagg ttttccgcca gacgccattg ctgccaggcg ctgatgtgtc cggcttctga
    27361 ccatgcggtc gcgtttggtt gcactacgcg taccgttagc cagagttgtc cggtgctttc
    27421 cggctgcggt agttcaggca gttcaatcac ctgtttatct tgtggagcaa catccagagg
    27481 cacttcaccg cttgccagcg gcttgccatc gagcgccacc gaccagtgca ggagttcgtt
    27541 atcgctatga cggaacaggt attcgctggt cacttcgatg ttccgcccgg ataaacggaa
    27601 ctggaaaaac tgctgttggt gttttgcttc cgtcagcgcc ggatgcggcg tgcggtcggc
    27661 aaagaccaga ccgttcatac agaactggcg atcgttcggc gtatcgccaa aatcaccgcc
    27721 gtaagccgac cacggattgc cgtttgcatc atatttgatt agtgactgat ccacccaatc
    27781 ccagacgaag ccgccctgta aacgggggta ctgacgaaac gcctgccagt atttagcgaa
    27841 gccgccaaga ctgttaccca tcgcgtgggc atattcgcaa aggatcagcg ggcgcatttc
    27901 tccaggcagc gaaagccatt ttttgatgga ccatttcggc accgccggga agggctggtc
    27961 ttcatccacg cgcgcgtaca tcgggcaaat aatatcggtg gccgtggtgt cggctccgcc
    28021 gccttcatac tgtaccgggc gggaaggatc gacagatttg atccagcgat acagcgcgtc
    28081 gtgattagcg ccgtggcctg attcattccc cagcgaccag atgatcacac tcgggtgatt
    28141 acgatcgcgc tgcaccatcc gcgttacgcg ttcgctcatc gcgggtagcc agcgcggatc
    28201 atcggtcaga cgattcattg gcaccatgcc gtgggtttca atattggctt catccaccac
    28261 atacaggccg tagcggtcgc acagcgtgta ccacagcgga tggttcggat aatgcgaaca
    28321 gcgcacggcg ttaaagttgt tctgcttcat cagcaggata tcctgcacca tcgtctgctc
    28381 atccatgacc tgaccatgca gaggatgatg ctcgtgacgg ttaacgccgc gaatcagcaa
    28441 cggcttgccg ttcagcagca gcagaccatt ttcaatccgc acctcgcgga aaccgacgtc
    28501 gcaggcttct gcttcaatca gcgtgccgtc ggcggtgtgc agttcaacca ctgcacgata
    28561 gagattcggg atttcggcgc tccacagttc cggattttca acgttcaggc gtagtgtgac
    28621 gcgatcggca taaccgccac gctcatcgat aatttcaccg ccgaaaggcg cggtgccgct
    28681 ggcgacctgc gtttcaccct gccacaaaga aaccgtcacc cgcagttcat cgcgcagctc
    28741 gccgtacatc tgaacttctg cctccagtac cgcgcggctg aaatcatcat taaagagagt
    28801 ggtaacttgg aaatcgctga tttgcgtggt cggtttatgc agcaacgaga cgtcacggaa
    28861 aatgccgctc atccgccaca tatcctgatc ttccagataa ctgccgtcac tccagcgcag
    28921 caccatcacc gcgaggcggt tttctccggc gcgtaaaaat gcgctcaggt caaattcaga
    28981 cggcaaacgg ctgtcctggc cgtaaccgac ccagcgcccg ttgcaccaca gatgaaacgc
    29041 cgagttaacg ccatcaaaaa taattcgcgt ctggccttcc tgtagccagc tttcatcaat
    29101 attaaatgtg agcgagtaac aacctgtcgg attctccgcg ggaacaaacg gcggattgac
    29161 cgtaatggga taggtcacgt tggtgtagat aggcgcatcg taaccgtgca tctgccagtt
    29221 tgaggggacg acgacagtat cggcgtcagg aagatcgcac tccagccagc tttccggcac
    29281 cgcttctggt gccggaaacc aggcaaagcg ccattcgcca ttcaggctgc gcaactgttg
    29341 ggaagggcga tcggtgcggg ccttttcgct attacgccag ctggcgaaag ggggatgtgc
    29401 cgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaatacgac
    29461 ggccagagaa tccgtaatca tagtcatagc tgtatcctgt gtgaaattgt tatccgctca
    29521 caatttcaca caacatacga gccggacaca tagagtgtaa agactggggt gcctaatgag
    29581 tgagctgact cacattaatt gtgttgcgct cactgcccgc tttccagtcg ggaaacttgt
    29641 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
    29701 ggaagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc cttcaccgcc
    29761 tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc
    29821 tgtttgatgg tggttaacgg cgggatataa cacgagctgt cttcggtatc gtcgtatccc
    29881 actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc
    29941 agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc atttagcatt
    30001 tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc tgttatcggc
    30061 tgaatttgat tgcgtgtgag atatttatgc cagcccgcca gacgcagacg cgccgagaca
    30121 gaacttaatg ggcctgctaa cagcgcgatt tgctggtgac ccaatgcgac caggtgctcc
    30181 acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg agtctggtca
    30241 gagacatcaa gaaataacgc cggaacatta gcgcaggcag cttccacagc aatggcatcc
    30301 tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc
    30361 accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca
    30421 cccagttgat cggcgcgaga ttttatcgcc gcaacaattt gcgacggcgc gtgcagggcc
    30481 agactggaag tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg
    30541 cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttctcg cgttttcgca
    30601 gaaacgtggc aggcctgatt caccacgcgg gaaacggtct gataagagac accggcatac
    30661 tccgcgacat cgtatagcgt tactggtttc acattcacca ccctgaattg actctcttcc
    30721 gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt cggcggggct gacgccgaag
    30781 tgagatttaa aatgctgacg ccaggtcaca ggtgagagaa aacccacgcc aatacaccta
    30841 cggcgtttac aggtatactc gctaaaaatt attcagcggg tttggaaaca aagatggcaa
    30901 aacttacctt acaagagcag ttgctcaaag cgggactagt caccagcaaa aaagcggcga
    30961 aggtcgagag aacggcgaaa aaatcgcgtg ttcaggcgcg tgaagctcgg gcggcggtag
    31021 aagaaaataa aaaggcacag cttgagcgtg ataaacagct tagcgagcaa caaaaacaag
    31081 cggcgttggc gaaagaatat aaagcccagg tgaagcagct cattgaaatg aaccgaatta
    31141 ccattgccaa tggcgatatt ggttttaact tcactgacgg caatctgatt aagaaaatct
    31201 ttgtcgataa aatcacgcaa acgcagttga ttaatggtcg tcttgcgatt gcccgcttgt
    31261 tggtcgataa caataacgaa ggtgaatacg tcattattcc cgccagcgtt gccgataaaa
    31321 ttgctcagcg tgatgccagc agtattgtgt tgcacagcgc gctcagcgcc gaagagcagg
    31381 atgaagacga tccgtatgcc gacttcaaag tgcctgatga tttgatgtgg taaacataga
    31441 taaatcgtgt gcagatgcct gatgcgacgc ttttcgcgtc ttatcaggcc tacggtttac
    31501 gagcgaaatg tgggccggat aaggcgttta cgccgcatcc ggcagtcgtg cactattatc
    31561 aacgcatatt cagtttattg gcgtggtacg caatatgctc gccaataaaa ctggagacaa
    31621 aataatagct gtgatcataa ccctcgtgat aacggattaa cgtcttgata ttcatctcct
    31681 ggcagatctt ttcaagattt ggagtccgca gctgttctgc gtaaaaatca tcactcaatc
    31741 cctgatcaac catgatttcc gcaacgcgtt gaccttgtga aataagactc accgggtcgt
    31801 aatccaacca ggcatcttta ttttcaccaa gatatgcggc aaaggcttgc tgtccccacg
    31861 gtacttgcga tggggagaca atgggcgaaa acgccgagac gctgacatat tcatctgggt
    31921 tacgtaacgc cagcaccagc gcgcccagcc cgcccataga atgacctgag atagactttc
    31981 tggccgttgc tggaaaatga tgcatcacta aattcggcag ctcgttgcgg atatagtcat
    32041 acattttgta atgttcattc cacggcgctt gcgtcgcgtt caggtaaaac ccggcacctt
    32101 gcccgagatc gtaacggtca gcatctgcga catgactgcc tcgcggactg gtgtccggcg
    32161 caacaacaat aatgttgtgc tcagccgcgt aacgctgcat ccccgattta gtaatgaaat
    32221 tttgctcgtt gcaggtcagg cctgaaagcc agtacaacac cggcaatttt tcattctcgg
    32281 cttttggtgg gagatagacg ccgacattca tttcacattt cagtgattgg gaataatgcc
    32341 gatacacatt ttgccagccg ccaaagctgg catatttttc aatgagttcc atcagtcatt
    32401 atctcacggt taattccccg gacggctgcc cggggagagt attaagaacc ccgtctgaac
    32461 tggacggggt aaaccagcgg gaaatcagta acgaattacg gttcgaatgg atttaccttc
    32521 atgcatcagg tcgaaggcgt cattaatttc atccaggctc atggtatgcg tgacaaacgg
    32581 ttccagatcg atatcacctt tcatcgcatc ttcaaccata cccggtaact gggaacgacc
    32641 tttcacgccg ccaaacgcgg aacctttcca tacgcgaccg gtgaccaact ggaatggacg
    32701 ggtggagatt tcctgaccgg agcctgctac cccgatgatc accgactgac cccagccgcg
    32761 gtgcgcactt tccagcgcag cacgcatcac gttgacgtta ccgatgcatt caaaggtatg
    32821 gtcgataccc catttgttga tatccagcag aacatcttta attggtttgt cgtagtcatt
    32881 cgggttaatg cagtcggtgg caccgaagcg acgcgccagt tcgaatttct tcgggttggt
    32941 atcgatagcg ataatccgtc ccgctttcgc ctgacgcgcg ccctgaacca ctgccagacc
    33001 aatcgcgcca agaccaaaca cggcaacaga atcacctggc tggactttag ctgtgttgtg
    33061 taccgcgcca ataccggtgg tcacgccaca gcccagcagg cagacgtgtt catggtttgc
    33121 ttctggatta attttggcca gagacacttc cgctaccacg gtgtattcac tgaatgttga
    33181 gcatcccatg tagtgataaa gcggctgccc gttgtaagaa aaacgggtgg tgccgtctgg
    33241 catcaagcct ttgccctgcg tttcgcgaac cgcgacacac aggttggttt tgccagaacg
    33301 acagaactca cactcgccgc actctgcggt gtaaagcggg atcacatggt cgccaggttt
    33361 gacgctggtt acgccttcac cgacttcaac aacaaccccg gccccttcgt gaccgagaac
    33421 caccgggaag acaccttccg ggtcatcgcc ggagagggta aatgcgtcgg tatggcaaac
    33481 accggtatgg gtgactttaa tcagcacttc accttttttc ggtggtgcaa cgtcaatttc
    33541 aacgatttcc agcggtttac cgggagcaaa tgcaacggca gcacgtgatt tcatctctcg
    33601 ctcttcctca atatggtaat agattcagct atttaagata ggcacgaacc agttcaatag
    33661 tgtcgtcaac ggattggctg acttcgcggc tgtagcagtc atttcggtca aacgtttccc
    33721 ggatatggct ttcaagcact tctgccatca gcccgttagc cgcgccccga acggcagcga
    33781 tctgttggag tatggcacgg cattcggcat caccctccag cgaccgttcc agagcatcaa
    33841 tctgcccccg aatacgacga actcgagtaa ggaccttttt cttctcttcc ggagtactgg
    33901 gcatttcgca cctcatcatc tgcatgcaat atactatagg ggggtatgct atatgccaat
    33961 gcataccccc ctatagtata taacactgga gaataaaatt tatccggccc cagtatttgt
    34021 aggcatgata agaggcgtga gcgccacatc aggcgatgtt tgttaaaagt agcaaaatgc
    34081 gcaaactcgc taaccattgc cgattttttt ctaaaaacgc tttgttatta acaagacgtt
    34141 ttaattcctt tatcaaactt acccgcaacg aaatactgct cccataacaa gacaagggag
    34201 cagacaatca tggcaatttc atcgcgtaac acacttcttg ccgcactggc attcatcgct
    34261 tttcaggcgc aggcggtgaa cgtcaccgtg gcgtatcaaa cctccgccga accggcgaaa
    34321 gtggctcagg ccgacaacac ctttgctaaa gaaagcggag caaccgtgga ctggcgtaag
    34381 tttgacagcg gagccagcat cgtgcgggcg ctggcttcag gcgacgtgca aatcggcaac
    34441 ctcggttcca gcccgttagc ggttgcaacc agccaacagg tgccgattga agtcttcttg
    34501 ctggcgtcaa aactgggtaa ctccgaagcg ctggtggtaa agaaaactat cagtaaaccg
    34561 gaagacttga ttggtaaacg catcgccgta ccgtttatct ccaccaccca ctacagcctg
    34621 ctggcggcgc tgaaacactg gggtattaaa cctgggcaag ttgagattgt gaacctgcag
    34681 ccgcccgcga ttatcgccgc atggcagcgg ggagatattg atggtgctta tgtctgggca
    34741 ccggcggtta acgccctgga aaaagatggc aaggtgctga ccgattctga acaggtcggg
    34801 cagtggggcg cgccgacact ggacgtctgg gtcgtgcgga aagattttgc cgagaaacat
    34861 cctgaggtcg tgaaagcgtt cgctaaaagc gccatcgatg ctcagcaacc gtacattgct
    34921 aacccagacg cgtggctgaa acagccggaa aacatcagca aactggcgcg gttaagcggc
    34981 gtgccggaag gtgacattcc ggggctggtg aaggggaata cctatctgac gccgcagcag
    35041 caaacggcag aactgaccgg accggtgaac aaagcgatca tcgacaccgc gcagtttttg
    35101 aaagagcagg gcaaagtccc ggctgtagcg aatgattaca gccagtacgt tacctcgcgc
    35161 ttcgtgcaat aaaaggaggc gcagatgctg caaatttctc atctttacgc cgattatggc
    35221 ggcaaaccgg cactggaaga tatcaacctg acgctggaaa gcggcgagct tctggtagtt
    35281 ctggggccgt ctggctgcgg caaaactacg ttgctgaatt tgattgctgg ttttgtgcct
    35341 tatcagtatg gcagcattca actggcgggt aagggtattc agggaccggg agcagagcgt
    35401 ggcgtagttt ttcagaatga agggttgtta ccgtggcgca atgtacagga caacgtggcg
    35461 ttcggcctgc aactggcagg tgtagagaaa atgcagcgac tggaaatcgc acaccagatg
    35521 gtgaaaaaag tcgggctgga aggcgcagaa aaacgctaca tctggcagct ttccggtggt
    35581 caacgtcagc gggtggggat tgcccgcgcg ttggcggcaa atccccagct gttattactc
    35641 gacgaaccgt ttggtgcgct ggacgccttc acccgcgacc agatacaaac cctgctgctg
    35701 aaactctggc aggagacggg aaagcaggtg ctgttgatta cccacgatat agaagaagcg
    35761 gtgtttatgg cgactgaact ggttctgctt tcacccggcc caggccgcgt gctggagcgg
    35821 ctgccgctca acttcgcccg ccgctttgtt gcgggagagt cgagccgcag catcaagtcc
    35881 gatccacaat tcatcgccat gcgcgaatat gttttaagcc gcgtatttga gcaacgggag
    35941 gcgttctcat gagtgtgctc attaatgaaa aactgcattc gcatcggctg aaatggcgct
    36001 ggccgctctc gcgtcaggtg accttaagca ttggcacgtt agcggtttta cttactgtat
    36061 ggtgggcagt cgcagcgctg caactgatta gcccgctgtt tttgccgcca ccacaacagg
    36121 tactggcaaa actgctcacc attgccgggc cgcagggatt tatggacgcc acgctgtggc
    36181 agcatctggc cgccagcctg acgcgcattt tgctggcgct actcgcggcg gtgttgatcg
    36241 gtattccggt cgggatcgcc atggggctaa gcccgacggt gcgcggcatt ctggatccga
    36301 taatcgaact ttatcgtccg gtgccgccgc tggcttattt gccgctgatg gtgatctggt
    36361 ttggtattgg tgaaacctcg aagatcttac tgatctattt agcgattttt gcaccggtgg
    36421 cgatgtcggc gctggcgggg gtgaaaagcg cgcagcaggt tcgcattcgt gccgccctgt
    36481 ctctgggtgc cagccgtgcg caggtgctgt ggtttgtcat tttgcccggc gcgctgccgg
    36541 aaatcctcac cggattacgt attggtctgg gtgtgggctg gtctacgctg gtggcggcgg
    36601 agctgattgc cgcgacgcgc ggtttaggat ttatggttca gtcagcgggt gaatttctcg
    36661 cgactgacgt ggtgctggcg gggatcgcgg tgattgcgat tatcgccttt cttttagaac
    36721 tgggtctgcg cgcgttacag cgccgcctga cgccctggca tggagaagta caatgagtga
    36781 acgtctgagc attaccccgc tggggccgta tattggcgca caaattacgg gggccgactt
    36841 aacgcgcccg ttaagcgata atcagtttga acagctttac catgcggtgc tgcgccatca
    36901 ggtggtgttt ctgcgcgatc aagctattac gccgcagcag caacgcgcgc tggcccagcg
    36961 ttttggcgag ctgcatattc accctgttta ccctcatgct gaaggggttg acgagatcat
    37021 cgtcctggat acccataacg ataatccgcc ggataacgac aactggcaca ccgatgtaac
    37081 atttattcaa acgccacccg caggggcgat tctggcggcg aaagagttac cctcgaccgg
    37141 cggcgatacg ctctgggcca gtggtattgc cgcatatgag gcgctctctg ttcccttccg
    37201 ccagctgttg agcgggctgc gtgcggagca tgatttccgt aaatcgttcc cggaatacaa
    37261 gtaccgcaaa accgaggctg agcatcaacg ctggcgtgag gcggtcgcga aaaatccacc
    37321 gttgctacat ccggtggtgc gaacgcatcc ggtgaccggt aaacaggcgc tgtttgtgaa
    37381 cgaaggtttt accacacgaa ttgttgatgt cagcgagaaa gagagcgaag ccttgttagg
    37441 ttttttgttt gcccatatca ctaaaccgga gtttcaggtg cgctggcgct ggcaaccgaa
    37501 tgatattgcg atttgggata accgcgtgac tcagcactat gccaatgcgg attacctgcc
    37561 acaggggcgg attatgcatc gggcgacgat tcttggggat attccgtttt atcgggcggg
    37621 gtaaggggga aatgccgggt tggataaccc ggcataatga tcattaacgc agaatcttct
    37681 tctcagccaa atccagcgca aagtagctga aaatcagatc cgcacccgca cgcttaatcg
    37741 aacctaagct ttcgagcacg actttctctt catctatagc acccgccagc gcggcgaact
    37801 taatcatcgc gtactcaccg ctcacctgat acgcgccaat cggcaattca gtacgttcac
    37861 gcagctcacg cacgatgtcg aggtatgctc cggcaggttt aaccatcagg cagtctgcgc
    37921 cctgggcttc atccagcagc gactcacgaa tcgcctcacg acggttcatt gggttcatct
    37981 gatagctttt gcggtcgcct tttaatgcgc ttccggcagc ttcacggaac gggccataaa
    38041 aggaggaggc gaacttggtc gaatacgaca taatcgccgt atctttaaag cccgcagcgt
    38101 ccagcgcctg gcgaatcgcc tgtacctgac catccatcgc ggcagaaggg gcgatgaagt
    38161 ctgcgcctgc agcagctgca accacagctt gcttgcctaa attttccaga gtcgcgtcgt
    38221 tgtcgacgcc atgctcgcac agaacaccgc agtgaccgtg agatgtgtat tcacagaagc
    38281 aggtgtctga cataacgatc atttctggca cggtctgctt gcagatgcgc gacatacgcg
    38341 ccaccagtcc atcttcccgc caggcatcgc tgccggtttc atcggtatgg tgagagatgc
    38401 cgaaagtcat cacggaacga ataccggcgt tggcgatgcg ttcaatttcg cgtgccagat
    38461 gtttctctgg aatgcgcatc acgcctggca tggcttcaac ggctttgtag tcgtcaattt
    38521 cttcttcaac aaagatcggc aacaccaggt cgttaaggct aagtgttgtc tcttcaaaca
    38581 tagcgcgcag cgcaggagat ttgcgcaggc gacgagggcg ttggattaaa tctgtcatgg
    38641 tctgcctgat gtttgtggaa tcgaggagca tagtatacct gaagcaggct gggtgtgttt
    38701 tacgaaagtt gtcgcgatgt tgacaaaaag agaatgaaaa agaggtcagg acatcttt
//
LOCUS       DAZFJL010000026        37973 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFJL010000026 DAZFJL010000000
VERSION     DAZFJL010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.026"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(161..448)
                     /gene="yebO"
                     /locus_tag="ACHJ8C_001362"
     CDS             complement(161..448)
                     /gene="yebO"
                     /locus_tag="ACHJ8C_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO5551247.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFLNRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(523..666)
                     /gene="mgrB"
                     /locus_tag="ACHJ8C_001363"
     CDS             complement(523..666)
                     /gene="mgrB"
                     /locus_tag="ACHJ8C_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO5551248.1"
                     /translation="MKKFRWVALVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            825..1064
                     /gene="yobH"
                     /locus_tag="ACHJ8C_001364"
     CDS             825..1064
                     /gene="yobH"
                     /locus_tag="ACHJ8C_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO5551249.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1208..1999)
                     /gene="kdgR"
                     /locus_tag="ACHJ8C_001365"
     CDS             complement(1208..1999)
                     /gene="kdgR"
                     /locus_tag="ACHJ8C_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO5551250.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     MQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2176..3549
                     /gene="yebQ"
                     /locus_tag="ACHJ8C_001366"
     CDS             2176..3549
                     /gene="yebQ"
                     /locus_tag="ACHJ8C_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5551251.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIVIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLIAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3595..4476)
                     /gene="htpX"
                     /locus_tag="ACHJ8C_001367"
     CDS             complement(3595..4476)
                     /gene="htpX"
                     /locus_tag="ACHJ8C_001367"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO5551252.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4668..6716)
                     /gene="prc"
                     /locus_tag="ACHJ8C_001368"
     CDS             complement(4668..6716)
                     /gene="prc"
                     /locus_tag="ACHJ8C_001368"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO5551253.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6736..7434)
                     /gene="proQ"
                     /locus_tag="ACHJ8C_001369"
     CDS             complement(6736..7434)
                     /gene="proQ"
                     /locus_tag="ACHJ8C_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO5551254.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRSQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7531..8028)
                     /gene="msrC"
                     /locus_tag="ACHJ8C_001370"
     CDS             complement(7531..8028)
                     /gene="msrC"
                     /locus_tag="ACHJ8C_001370"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO5551255.1"
                     /translation="MNKAEFYADLNRDFNALMVGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8158..9441
                     /gene="yebS"
                     /locus_tag="ACHJ8C_001371"
     CDS             8158..9441
                     /gene="yebS"
                     /locus_tag="ACHJ8C_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO5551256.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGAMVFFCVIGAPLILVTSIAYLWFGNQLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9410..12043
                     /gene="yebT"
                     /locus_tag="ACHJ8C_001372"
     CDS             9410..12043
                     /gene="yebT"
                     /locus_tag="ACHJ8C_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO5551257.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLSSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPGGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12123..13562
                     /gene="rsmF"
                     /locus_tag="ACHJ8C_001373"
     CDS             12123..13562
                     /gene="rsmF"
                     /locus_tag="ACHJ8C_001373"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO5551258.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLQLTAPYGWTLTPIPWCEEGFWIERDSEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13680..13916
                     /gene="yebV"
                     /locus_tag="ACHJ8C_001374"
     CDS             13680..13916
                     /gene="yebV"
                     /locus_tag="ACHJ8C_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO5551259.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            14021..14212
                     /gene="yebW"
                     /locus_tag="ACHJ8C_001375"
     CDS             14021..14212
                     /gene="yebW"
                     /locus_tag="ACHJ8C_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO5551260.1"
                     /translation="MFALVLFICYLDGGCEDIVVDIYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14213..14869)
                     /gene="pphA"
                     /locus_tag="ACHJ8C_001376"
     CDS             complement(14213..14869)
                     /gene="pphA"
                     /locus_tag="ACHJ8C_001376"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO5551261.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRSVRGNHEQMAMDALASRQMSLWLINGGD
                     WFIALADNQQKQAKTALEKCQHLPFILELHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGIAGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15264..15605)
                     /gene="yebY"
                     /locus_tag="ACHJ8C_001377"
     CDS             complement(15264..15605)
                     /gene="yebY"
                     /locus_tag="ACHJ8C_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO5551262.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGDALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15618..16490)
                     /gene="copD"
                     /locus_tag="ACHJ8C_001378"
     CDS             complement(15618..16490)
                     /gene="copD"
                     /locus_tag="ACHJ8C_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO5551263.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGAGWSDVFSPNIWLAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLVSGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16494..16868)
                     /gene="yobA"
                     /locus_tag="ACHJ8C_001379"
     CDS             complement(16494..16868)
                     /gene="yobA"
                     /locus_tag="ACHJ8C_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO5551264.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            17007..17237
                     /gene="holE"
                     /locus_tag="ACHJ8C_001380"
     CDS             17007..17237
                     /gene="holE"
                     /locus_tag="ACHJ8C_001380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO5551265.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17339..17995
                     /gene="yobB"
                     /locus_tag="ACHJ8C_001381"
     CDS             17339..17995
                     /gene="yobB"
                     /locus_tag="ACHJ8C_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO5551266.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSYGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            18019..18681
                     /gene="exoX"
                     /locus_tag="ACHJ8C_001382"
     CDS             18019..18681
                     /gene="exoX"
                     /locus_tag="ACHJ8C_001382"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO5551267.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18678..20738)
                     /gene="ptrB"
                     /locus_tag="ACHJ8C_001383"
     CDS             complement(18678..20738)
                     /gene="ptrB"
                     /locus_tag="ACHJ8C_001383"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO5551268.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20947..21606)
                     /gene="yebE"
                     /locus_tag="ACHJ8C_001384"
     CDS             complement(20947..21606)
                     /gene="yebE"
                     /locus_tag="ACHJ8C_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO5551269.1"
                     /translation="MANWLNQLQSLLGQSRSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEKGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(21933..22289)
                     /gene="yebF"
                     /locus_tag="ACHJ8C_001385"
     CDS             complement(21933..22289)
                     /gene="yebF"
                     /locus_tag="ACHJ8C_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO5551270.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22356..22646)
                     /gene="yebG"
                     /locus_tag="ACHJ8C_001386"
     CDS             complement(22356..22646)
                     /gene="yebG"
                     /locus_tag="ACHJ8C_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO5551271.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22780..23958
                     /gene="purT"
                     /locus_tag="ACHJ8C_001387"
     CDS             22780..23958
                     /gene="purT"
                     /locus_tag="ACHJ8C_001387"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO5551272.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSENLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSLLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(24014..24655)
                     /gene="kdgA"
                     /locus_tag="ACHJ8C_001388"
                     /gene_synonym="eda"
     CDS             complement(24014..24655)
                     /gene="kdgA"
                     /locus_tag="ACHJ8C_001388"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO5551273.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24692..26503)
                     /gene="edd"
                     /locus_tag="ACHJ8C_001389"
     CDS             complement(24692..26503)
                     /gene="edd"
                     /locus_tag="ACHJ8C_001389"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO5551274.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDNNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26738..28213)
                     /gene="zwf"
                     /locus_tag="ACHJ8C_001390"
     CDS             complement(26738..28213)
                     /gene="zwf"
                     /locus_tag="ACHJ8C_001390"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO5551275.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28551..29420
                     /gene="hexR"
                     /locus_tag="ACHJ8C_001391"
                     /gene_synonym="yebK"
     CDS             28551..29420
                     /gene="hexR"
                     /locus_tag="ACHJ8C_001391"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO5551276.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGTKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29548..30990
                     /gene="pyk"
                     /locus_tag="ACHJ8C_001392"
     CDS             29548..30990
                     /gene="pyk"
                     /locus_tag="ACHJ8C_001392"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO5551277.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQEAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31122..32093)
                     /gene="lpxM"
                     /locus_tag="ACHJ8C_001393"
                     /gene_synonym="msbB"
     CDS             complement(31122..32093)
                     /gene="lpxM"
                     /locus_tag="ACHJ8C_001393"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO5551278.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMVMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32212..33534)
                     /gene="mepM"
                     /locus_tag="ACHJ8C_001394"
     CDS             complement(32212..33534)
                     /gene="mepM"
                     /locus_tag="ACHJ8C_001394"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO5551279.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33550..34482)
                     /gene="znuA"
                     /locus_tag="ACHJ8C_001395"
     CDS             complement(33550..34482)
                     /gene="znuA"
                     /locus_tag="ACHJ8C_001395"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO5551280.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLENVKPLLMKSIHDDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            34561..35316
                     /gene="znuC"
                     /locus_tag="ACHJ8C_001396"
     CDS             34561..35316
                     /gene="znuC"
                     /locus_tag="ACHJ8C_001396"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO5551281.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35313..36098
                     /gene="znuB"
                     /locus_tag="ACHJ8C_001397"
     CDS             35313..36098
                     /gene="znuB"
                     /locus_tag="ACHJ8C_001397"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO5551282.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(36243..37253)
                     /gene="ruvB"
                     /locus_tag="ACHJ8C_001398"
     CDS             complement(36243..37253)
                     /gene="ruvB"
                     /locus_tag="ACHJ8C_001398"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO5551283.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37262..37873)
                     /gene="ruvA"
                     /locus_tag="ACHJ8C_001399"
     CDS             complement(37262..37873)
                     /gene="ruvA"
                     /locus_tag="ACHJ8C_001399"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO5551284.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         9110 a         9788 c         9334 g         9741 t
ORIGIN      
        1 atatcttttt ctgatatccc gttacgcaaa ttaataatat gataattatt tgaagtcaga
       61 ccagagtttt aatttgaagc agctgcacca tgacagtgcg tataatcagc atgaattgag
      121 taacccttca acgacggggt tactcaagat tgaggaagga ttatcgttcg gcgaccagac
      181 gaataatatc ttcgtcttca accgattttt gactctcaac ggtcttttta tctgctttct
      241 caggttcgtt tgcttcgcac aaacgacgta acagtgcatt ttgacgtttt tgctgatcca
      301 gcaacgcctc aagcagttca atctgttcgt tagtccgtga actggcacga ttgagaaaaa
      361 accacaagat gagcccgata agaagaacca ccaccgatac aaccaaagac gcaatgttca
      421 tcacgcctga atttacaact tcgttcattt caccacctct gagtagaggc gctattctac
      481 cactgctgga gaggaagaaa atctagtgct gaaaaaatga tatcaccacg ggataaactg
      541 gttaatggca caaattccgc tgaaaaattg tacatcctga tcgcacatca tgttgaatac
      601 ctgcgcccaa agcagcaagc aagccaacac cacgacaacc agagcgaccc atcgaaactt
      661 tttcactcca atctccgttt cacctacctt atgtcattaa actagcatgc gttagttaaa
      721 cagcgcctaa ctatgtcgat attcgtgctt tttcggaatg agtcgcttgc ttcagcgcat
      781 attgccgcta cgattaagcg aacataaaaa gagaagaggt tgtaatgcga ttcatcattc
      841 gcacagttat gctgatagcg ctggtatgga taggattatt actcagcgga tatggcgttt
      901 tgattggaag caaagagaat gccgcgggat taggtttaca atgtacctat ctgaccgcca
      961 gaggcaccag cacggtacaa tatctgcaca ctaaaagtgg ttttctgggg ataacagatt
     1021 gcccgttgtt acgtaaaagc aatattgtgg tcgataatgg ttaagggtaa taaataacca
     1081 gattctcact catgagtaaa atattcttat cgcaagtgcg tttaatataa cgccaccttc
     1141 aactttattt ttctcatgct aaataaagaa cctgtctgtt tgatattcag acaggttctt
     1201 actctcatca gaacggatag tcgtgataac ccatttgggc agaaattttg cgcgccgcgg
     1261 tatgcaacat tgcgacatat tcctgtaaac gctcttcaga gaaacgcaac gtcgggaagg
     1321 agatgctcag accggcaatg accacgccaa agcgatcaaa taccggaacc gcaatgcaac
     1381 gcagtccttc ttcctgctct tcattatctt cgccataccc ctgctcgcgc acctggtcca
     1441 gaacgggtaa taacgcttct gtactggtga tggtccgctc ggtactgcgt ttatactcca
     1501 cgccctcaag aatttgcatc acttcatcgc gatcgcgcca tgccagcagt accttaccaa
     1561 tcgcggtgct gtacagcgga ttacgacgcc caatccgtga atacatgcgc aaattgtaca
     1621 ttgagtcaat tttgtggatg taaacaatac tgtcttcgtc cagtgcgccg aggtggatag
     1681 tttctttggt caggcgggag atctcacgca tctggatatc tgcgctacga attaaatcga
     1741 cgttttgcaa cgcgcgagcg cccagttcaa acaatttcag ggtcagcgaa tatttctccg
     1801 actccccttc ctgcgccaca taacctaagg ttttcatggt ctgtaaaaag cgataaacgg
     1861 tgctttttga catcatgacg cgctgcgaca actcggttat ccctatttcg cgctcttcac
     1921 ccagcgcctg caaaatgcca aaaactttta gcacggaaga tacagaatca ggctgtttat
     1981 ccagatctgc gttagccatt tatcacctca ttgcgagtgt tttataaaaa tcagaactgt
     2041 tttttattat aatttcgcac cagggtggtc gcaatccatc ttttgccggt tagttacaat
     2101 tctgcgacat ccaccgtgaa tatcagtgct agaatcagac ccctgttgat tattcaccaa
     2161 agatataaaa ttcctatgcc aaaagttcag gccgacggcc tgccattgcc ccagcgatac
     2221 ggtgcgatat taaccattgt gattggtatt tcgatggctg tccttgacgg cgcaatcgcc
     2281 aacgtcgcct tgccaacaat cgctacggac cttcatgcca cgccggccag ttccatctgg
     2341 gtagtgaacg cctatcaaat cgtcattgtc atctccctgc tctcgttttc gtttctgggc
     2401 gatatgtttg gctatcgacg tatttataaa tgcggtctgg tcgtttttct gttgtcttca
     2461 ctgttctgcg ccctttctga ttcgctgcaa atgctcaccc ttgcgcgtgt catacaaggt
     2521 ttcggcggtg cagcgttgat gagcgttaat accgcactta tccgcctgat ctatccacaa
     2581 cgttttctgg gtagagggat gggcataaac tcgtttattg ttgccgtctc ttctgctgcc
     2641 gggccgacaa ttgctgcagc aatcctctcc atcgcatcct ggaaatggtt atttttaatc
     2701 aacgtaccgt tgggaattat cgccctgctt ctggcaatgc gttttctgcc acccaatggt
     2761 tctcgcgcca gtaaaccccg tttcgacctg cccagcgccg tgatgaacgc gttaaccttc
     2821 ggcctgctta ttactgcatt gagtggtttc gctcaggggc aatcgctgac attgattggt
     2881 gcggaactgg tggtaatggt tgtcgttggt attttcttta ttcgccgcca gctttctctt
     2941 cccgtaccgc tgctaccggt ggatttactg cgtatcccgc tgttttcact ttctatttgc
     3001 acatctgttt gctctttctg cgcacaaatg ctggcaatgg tttcccttcc cttttacctg
     3061 caaaccgtgc tcgggcgtag tgaagtcgaa acaggtttac ttctgacacc gtggccgtta
     3121 gcaacgatgg tgatggctcc actggcaggc tatttgattg aacgcgtaca tgcaggattg
     3181 ctgggtgctt tagggttatt catcatggct gcggggcttt tttccctggt tctgctgcca
     3241 gcgtcacctg cggatatcaa tattatctgg ccgatgatct tatgtggtgc tggatttggc
     3301 ttgttccagt cacccaataa ccacaccatt attacctccg ctcctcgcga acgtagcggt
     3361 ggagccagtg gcatgttagg gacagctcgt cttctgggtc agagtagcgg cgcggctctg
     3421 gtagcgctga tgctaaatca gtttggtgat aatggtacgc acgtctcgct gatagctgcg
     3481 gctattctgg cagtgattgc tgcctgtgtc agtggtttac gtatcactca gccacgatcc
     3541 agggcataaa aaaagcgcgt cgatcaggac gcgcttttta gtatttactt catattactt
     3601 caggtattca cccgtacgca gagcttcaat acgtttatcc agcggcgggt gagtcatgaa
     3661 caactcactg agcgatttcg acttcccgtt aatgcagaaa gccatcatgc tggttgcttc
     3721 ttgcggttca tagctggttt tcaggcgttg cagcgcggca atcattttct cgcgaccaac
     3781 cagttttgcc gaacccgcat cggcgtggaa ttcacgatga cgcgagaacc acatggtgat
     3841 aatgctcgcc agaataccaa acaccagttc cagaaccgtt gcaaccgcaa agtagatcag
     3901 cgggttgccg ttgctctctt caccttcatc acgatttccg cccataaaac ccgcggcaag
     3961 ttgcgccaga atacgggaaa taaagataac gaaggtgttc accacgccct gaatcagcgt
     4021 catggtgacc atatcaccat tggcgatgtg gctgatttcg tgagcaatta ccgcctcggc
     4081 ttcatccggg ctcatgttct gcagcaaacc ggtgctgaca gcaaccagag aggcatcacg
     4141 gcgcgcaccg gttgcaaaag cattgatgtc cggcgcatgg tagatagcca cttgcggcat
     4201 agcgatcccc gcctgacgag cctgggttgc tacagtattg accagccaac gttccctttc
     4261 gttacgcggt tgctcgatca cttccccgcc aacagatcgt aatgccatcc atttggacat
     4321 cagaagcgaa acgaaggaac caccaaaacc gaacagcaag gccatgatca tcagcccctg
     4381 aacgctgctc gactgtatcc ctgtcaggct cagtaccagc ccgaaaacga ccattacggc
     4441 caggttcgtt agcaggaaga gcgcgattcg catcataatt ttctttttac ctcagtttaa
     4501 caaaacgcaa tatgcgatac ccacatcgta tgggttacgc gactattttc aagtctggat
     4561 agtgcgtaag tcaccagaaa gacacaactt tacattttgt agcatctgat ttacggcatc
     4621 ttgccgctgt taaaaaatca ggcacaattt cttgtgcctg attgatatta cttgacggga
     4681 gcgggttgtt ccgcgggtct ggctttttca agcttcgcca gatcgagtgc gatattcacc
     4741 gtctcatcca gataaggatc tggctcctgg taatctttcg gtagatcatc cagtttcttc
     4801 aactccggtt taccttcgcg tttaaagcgt tcgttcaaac gcgccagacg cgtcgcatca
     4861 tcttcattat tctctttctc acgcacagcg taattcagag aaacgatatt gcgcttgtcc
     4921 ttcatagcgt tgaagcgcgc gatatccttc atgatgttct ggaactcagg atctttcgcg
     4981 atacgcgcat tatgttcctt cagcagctcc ggttcaaagg ccgttaaatc tcctgatttc
     5041 acataagtcg cggcatcaat gctatcccac ggcagcgcgt tatcttcgaa tttctcaccc
     5101 gtttccgttt cttcattacc cgtcggcatg atgatgtctg gcgttacgcc tttacgttgc
     5161 gtactgccgc cgttaacgcg atagaatttc tggatcgtgt actgcacaga acccagcgct
     5221 ggccattcag gacgtaacat ctgatcgtaa atacggttca atgaacggta ctgctgaacg
     5281 gtgcctttac caaatgtcgg ttcacccaca accagggcac gaccgtaatc ctgcattgcc
     5341 gcggcaaaga tttctgaagc cgaagcactg aagcggtcaa ccagcaccac cagcgggcct
     5401 ttatagaaga cctgtccgtc ggtgtcgctg tcttcacgaa ccttgccgtt gttatcgcgg
     5461 acctgaacaa tcgggccgga tggaatgaac agaccggaga gcgatacggc ttcagtcaac
     5521 gccccaccac cattgctacg caggtcgata atcacactgc tgacattctg tttttccagt
     5581 ttctgcagtt gcactttgac atcgtctgtc aaacctacat agaagcccgg aatatccagc
     5641 acgccgactt tctctttacc gacggtcttc accgacattt taaccgcgcg gtcttcgaga
     5701 cgaatacgtt cacgggtcaa cgttacagta cgcgtcttgg tccctttacc agcaggtaaa
     5761 atttccagac gaactttact gcccttaggc cctttaatta aggccaccac atcatcaaga
     5821 cgccagccaa tcacgtcaac catcggcttg cctgtttgac caacaccgac aattttgtca
     5881 ccaacgctga tagctttgct cttcgctgcc ggacctcctg ccaccatcga attgataacg
     5941 gtgtagtcat catccatttg cagcactgcg ccaatacctt ccagcgacaa actcatttca
     6001 gtgttgaact gttcggtatt acgcggggaa agatagttgg tatgcgggtc gatttcacgc
     6061 gcaaacgccg tcattgccag cgagaaaaca tcttcgctgt tggtttgcgc cagacgacga
     6121 atggcaaatt tgtagcggcg agtcagggtt tcacgaattt ccttatccgt ttttcctgtc
     6181 agcttcaggc ttaactcgtc gaatttgact ttactgtccc acagcgcgtt caactcagcc
     6241 tcgtttttcg gccagggcgc tttgctgcgg tcaaggttat aagtgtcgtt gccggtgaaa
     6301 tccatcggct tttccagtac cgacaaagcg tactggtaac gttcaaaacg gcgcttttgc
     6361 gccagattgt agagatcgta gaaaacgtcg agtttacctg aacgcagttc atcgcctaac
     6421 tcagttttct ttttcgcgaa ctgttcaaca tcgcttgcca gcagcacatt gtggctgtaa
     6481 tcgagcagat tcaggtagcg gtcaaagatt ttggccgaaa atgcctgatc gaggtcgaac
     6541 tggcgataat gagaacgggt gaagcgcgac gttacgcgct cactcaccgt cgcatgctgc
     6601 gtctcttcct ttaataccgg aatttgatca gcacgcgtga tatcttctac agcgaaggtc
     6661 tggcctgcta ttgcaagcag gccagctaac gcggtaagcc taaaaaacat gttcatgcct
     6721 ggcccggcct ccgtttcaga acaccaggtg ttctgcgcgc acaatcaaag acatacccga
     6781 attcagctgg acgcggacgc cgtctttggt gatttctaat acggtggcat ccatcgcgtt
     6841 ttgacccgct ttcaccttca gggcttgtcc gacagtcaga gctgaaatgt cagaaaccgg
     6901 ggtgtgctgt tcttcgcgag gtgcttttac tgtttttggc gctttctcta ccggcttttg
     6961 cgaacgaggt ttacgttcag cgccttcttt gcggcgtggc gtggtcggac gtggcttgcg
     7021 ttcgcggcgc ggtgcgtctt ctttctcacc agcagctgcg gcagcttcgc gttttttcgc
     7081 ttgctgttca gcacgctgtg cctgaacacg cgctttcgct tcttcaagct gcttgcgagc
     7141 atgctcgaca tgttgctcgt ccagctcacc gcatgggttg ccgtcaagat cgacacgtgt
     7201 tgcgccgggt ttaacaccgt aaagataacg ccagctcgaa gtgtagagac gtaaagcgga
     7261 tcgcaattgc gttttgctca ggttcatttc cccggcaaca cgatcgacca aatcctgaaa
     7321 aataccgatt ttcagcggac gcgcttcacc ttccgcactg aaacagtggg gaaaacgttc
     7381 ggccagaaac gcgattactt ctttactgct attcaactta ggttgatttt ccatgaaatt
     7441 tcctgattac aacggacgta gccaacaagc gcaggcatga acaggcgtca ttataatgac
     7501 gctatcagta aatgctacgt tatccgttga ttatcctgcg acgctcgcaa agaatttttt
     7561 gtaatccgtc gttgcaagca ctttttcaag ctgtgccaca agctgacgta agccttgctc
     7621 gtcctcgtct gtaaagcgac cgaagacggt actatcgatg tcgagaacac caataatctg
     7681 atttttcacc accagtggca gaacaatttc agaattactc gccgcatcac aggcaatatg
     7741 cccgtcaaac gcgtgaacat cctcgatacg ctgcacttga ttgcgggcaa ccgcagtgcc
     7801 gcatacgccg cgcccaacgg gtatccggac acaggcaatt ttgccctgaa atggtccgag
     7861 caccagtgta tcgtcctcaa gcaaataaaa acctgcccag tttacgtcag tgagacgctc
     7921 atataacaac gcactggtgt tcgcaagcgt tgccagaaaa ctggtttctc ccaccatcag
     7981 cgcgttaaag tcgcgattta aatccgcgta aaattctgct ttgttcatta tataatcact
     8041 tggttgtctt acctggaact gccagcctat taaaataagc attaaatgcg ttaatgctca
     8101 agatcattcc catcatgggt taagattaat gttaattctt attacatttg gcacgtcatg
     8161 gctcttaaca caccacaaat tacgccgaca aagaagataa cagtgagggc tatcggcgag
     8221 gaactgccgc gtggtgatta ccaacgttgc ccgcaatgtg acatgctgtt tagcctgccc
     8281 gagataaatt ctcatcaaag tgcctattgt ccgcgctgtc aggcaaaaat tcgtgacggg
     8341 cgcgactggt cgctaacgcg cctggcggca atggccttca ccatgctgtt gcttatgccg
     8401 tttgcctggg gcgaaccgct gttgcatatc tggctgttag gcattcgtat tgacgccaac
     8461 gttatgcaag gcatctggca aatgaccaaa cagggcgatg cgataacggg ggcgatggtc
     8521 tttttctgcg ttatcggtgc ccccctcatt ctggtgacct ccatagctta tttatggttt
     8581 ggtaaccaat tgggaatgaa tttacgcccg gtactgctga tgcttgagcg gctgaaagag
     8641 tgggtgatgc tggatatcta cctggtcggc attggcgttg cttctataaa ggtacaggat
     8701 tatgcccata tccaggcggg tgtcggcttg ttctcttttg tggcgttggt gattttaacg
     8761 acggtgacgt tgtcacatct taatgttgaa gaactgtggg agcgatttta tccacagcgc
     8821 cccgctaccc gtagggacga gaaactccgt gtctgtcttg ggtgccattt taccggctat
     8881 cctgatcagc gtggtcgctg cccgcgttgc catatcccgc tacgcctgcg tcgccgtcat
     8941 agtctgcaaa aatgctgggc ggcgctgtta gcgtcaatcg ttttgttgtt acctgccaac
     9001 ctgttgccta tctctatcat ttatctgaat ggtggtcggc aggaagatac gattctttcc
     9061 ggaattatgt cgctggcaag tagcaacatt gcagttgcgg gaatcgtgtt tatcgccagt
     9121 attctggtac cgtttactaa agtgattgtt atgttcactt tactgttgag tattcatttt
     9181 aaatgccagc aaggtttacg cacacgcatt ctgttactgc ggatggtgac ctggattggt
     9241 cgctggtcga tgctcgacct gtttgtcata tctttaacca tgtcgctgat taatcgcgat
     9301 caaatcctcg cttttactat ggggccggct gcgttttatt tcggcgcagc ggtaattttg
     9361 actattcttg ctgtggaatg gctggacagc cgcttacttt gggatgcaca tgagtcagga
     9421 aacgcccgct tcgacgactg aagcgcagat taaaaataaa cgccgtatct cacctttctg
     9481 gctgctgccg ttcatcgcgc taatgattgc tggttggctg atttgggaca gttatcagga
     9541 ccggggtaat accgtcacca tcgactttat gtcggcggat ggtattgttc caggccgtac
     9601 gcctgttcgt tatcagggcg ttgaagttgg aacagtgcag gatatcagcc tcagcgacga
     9661 tcttcgtaag attgaagtca aggtcagcat caagtccgat atgaaagatg cgctgcgcga
     9721 agagacacag ttctggctgg tgacgccaaa ggcctcgttg gcaggtgtct ccgggctgga
     9781 cgccctcgtc ggtggtaatt atatcggcat gatgccgggt aaaggtaaag agcaggatca
     9841 ctttgtcgca ctcgacaccc aaccgaaata tcggctggac aatggcgatc tgatgatcca
     9901 cctgcaagcc cccgatctcg gttcactgag cagtggttca ttggtctatt tccgcaagat
     9961 cccggtgggt aaagtctacg actatgccat caatcccaac aagcaaggcg tggtgattga
    10021 tgtcctgatc gagcggcgtt ttaccgatct ggtgaaaaaa ggtagccgtt tctggaacgt
    10081 ttccggcgtt gatgccaacg tcagtatcag tggcgcgaag gtgaaactgg aaagtctggc
    10141 ggcactggtt aacggtgcga ttgccttcga ttcacctgaa gagtcgaaac ctgccgaagc
    10201 ggaagatacc tttggtctgt atgaagacct ggcccatagc caacgtggcg taataataaa
    10261 actggaactg ccaggcggtg ccggattaac cgccgactcg acgccgttaa tgtatcaggg
    10321 gctggaagtc ggacagctga ctaaactgga tttaaatcct ggtggtaaag tcactgggga
    10381 aatgaccgtt gatcccagcg tcgtcaccct gcttcgtgaa aatacccgca tcgaattacg
    10441 taatccgaaa ttatccctta gcgatgctaa tctcagtgcc ctgctgaccg gcaaaacctt
    10501 cgaactggtg cccggcgatg gcgagccacg taaagagttc gtggttgtgc caggcgaaaa
    10561 agccctgctg caagagccgg atgttctgac actgaccctg acagcaccag aaagttacgg
    10621 tattgatgct ggtcagccgc tcattcttca cggcgtgcag gtaggccagg ttatcgatcg
    10681 taaactcacc agcaaaggcg tcacctttac cgtcgccatc gagcctcagc atcgcgaact
    10741 ggtaaaaggc gatagcaaat ttgtcgtcaa cagccgcgtc gacgtgaagg tagggctgga
    10801 tggcgttgag tttctcggtg ccagcgcctc agaatggatc aatggcggaa tacgtattct
    10861 gccgggcgat aaaggcgaga tgaaagccag ctatccactg tatgccaatc tggaaaaagc
    10921 gctggagaac agccttagcg atttacccac cacaaccgtg agtttgagtg cagagacgct
    10981 gccggatgtg caggcaggat cggtagtgct gtaccgtaaa tttgaagttg gtgaagttat
    11041 tactgtgcgt ccgcgagcta acgcgtttga tatcgatctg catattaagc cggagtatcg
    11101 caaccttctg accagcaaca gcgtgttctg ggcagaaggt ggggcgaaag ttcagctgaa
    11161 tggtagtggt ctgactgtac aggcatcccc gctctccaga gcattaaagg gggctattag
    11221 cttcgataac ctcagcggtg ccagcgccag tcagcgtaaa ggcgacaagc gtattctgta
    11281 tgcttccgaa acagcggccc gtgcggttgg cggacaaatt acgcttcacg ctttcgatgc
    11341 gggaaaactg gcggtcggga tgccaattcg ctatctcggt attgatatcg ggcaaatcca
    11401 gacgctggat ctgattaccg cacgcaatga agtacaggca aaggcggtgc tctatccgga
    11461 atatgtccag acctttgctc gcggtggtac gcgcttctca gtggtcacac cgcaaatttc
    11521 ggcagctggc gttgagcatc ttgatactat cctccagccg tatatcaacg tcgaaccagg
    11581 ccggggcaat cctcgccgcg actttgaatt acaagaggcc accattactg attcgcgtta
    11641 cctggatggc ttaagcatta ttgttgaagc gccggaagcc ggttcgttag gtatcggtac
    11701 acctgtgctg ttccgtggtc tggaagtagg tacggttacc ggaatgacgc tggggacatt
    11761 gtcagatcgc gtgatgattg cgatgcgcat cagtaaacgc tatcaacacc tggtgcgtaa
    11821 caattccgtc ttctggttgg catcgggtta cagtctggac tttggtctga cgggcggcgt
    11881 agtgaaaacc ggcaccttta accagtttat ccgtggcggc atcgccttcg ccacgcctcc
    11941 gggtacgcca ctggcaccga aagcccagga aggcaagcac ttcctgttgc aggaaagtga
    12001 accgaaagag tggcgtgaat ggggaactgc gcttcccaaa taatgcccac tgctccggcg
    12061 tgcctgcgcc ggagcgttta tgctaaactg cgcgcctgtt tttttgccag tggtatatgc
    12121 tcgtggccca acacaccgtt tatttcccgg acgcctttct gacacaaatg cgcgaagcga
    12181 tgccttcgac gctctctttt gatgattttc ttgccgcctg tcagcgcccg ttgcgccgca
    12241 gcattcgcgt taatacgctg aaaacctccg ttgctgattt cctgcaatta actgctcctt
    12301 atggctggac gcttacgcca attccgtggt gtgaagaagg tttctggatt gaacgcgaca
    12361 gtgaagatgc attgccgttg ggtagtaccg ccgagcattt aagcggcctg ttttatattc
    12421 aggaagccag ttcaatgttg cctgttgccg ccttgtttgc tgacggtaat gcaccacagc
    12481 gggtgatgga tgtcgctgct gcaccaggtt ccaaaacgac gcaaattgct gcgcggatga
    12541 ataacgaagg ggcaatcctt gccaatgagt tttccgccag tcgggtaaaa gtgttacatg
    12601 ccaatatcag ccgctgtggc atcagtaatg ttgcgctcac gcattttgat ggccgcgtgt
    12661 ttggtgcggc agtgccagaa atgttcgatg ccattttgct ggacgctccc tgctccggcg
    12721 aaggcgtggt gcgtaaagat cccgatgcgc taaaaaactg gtcaccagaa agcaatcagg
    12781 aaatcgcagc gacccaacgg gagctgatcg acagcgcctt tcatgcatta cgtcctggcg
    12841 gtacgctggt ttactcgacc tgtaccttaa accgggaaga aaacgaagcc gtttgcctgt
    12901 ggctgaaaga gacttatccc gacgcagtag agtttttacc gcttggcgat ctcttccctg
    12961 gtgcaaataa ggcgctgacc gaggaaggct ttttgcatgt tttcccacaa atttacgact
    13021 gcgaaggctt cttcgttgct cgtctgcgta aaactcaggc gattcccgcc ttacccaccc
    13081 caaaatacaa agtgggcaat ttcccgttca gcccggtgaa agatcgcgaa gccggtcaaa
    13141 ttcgtcaggc ggctgcaagt gttggcttaa actgggatga aaacctgcga ctctggcagc
    13201 gtgacaaaga actgtggttg ttcccggtag gcattgaagc cctgatcggt aaagtccgat
    13261 tttctcgctt ggggattaaa cttgccgaaa cgcacaacaa aggttatcgc tggcagcatg
    13321 aagccgttat tgcccttgcc tcccccgaca atgtgaacgc ttttgaactg acaccgcagg
    13381 aagcggaaga gtggtatcgc gggcgcgatg tttacccgca agccgcgcca gtggcggatg
    13441 atgtattggt taccttccag catcagccga ttggtttagc caaacggatt ggttcacgac
    13501 tgaaaaacag ctacccgcgt gaactggtgc gcgacgggaa actttttacc ggtaacgcct
    13561 gacagcgcac aaaaaaagcg cactttttga ctggcacatt caactgcctc aactaggctg
    13621 aaaaatggtg cgatcggact ggtcgtacca caaccggcag ctaaatggag agcacgaaga
    13681 tgaaaaccag tgtgcgcata ggcgcttttg aaatcgacga cggcgaatta cacggcgaat
    13741 cgccgggtga tcgaacgtta accattcctt gtaaatcaga ccccgattta tgtatgcaac
    13801 tggatgcctg ggatgctgaa accagtatcc ctgccctgct aaatggcgaa cactctgtcc
    13861 tttaccgtac ccgttacgat caacaatctg atgcctggat tatgcgtctt gcctgatcca
    13921 aaaagaaccc gtcggcatgg cgggttattt gtccgggtta ttcccccgtt gtaaaatctc
    13981 tcctaaactt aacggtacgg caccacactt cggggatgaa atgttcgcgc tggtactttt
    14041 tatttgctac ctggatggcg gttgtgaaga cattgttgtg gatatctaca acacggaaca
    14101 gcagtgtctt tattctatga gcgatcaacg gatccgccat ggcggttgtt ttccgattga
    14161 ggattttata gatggtttct ggcgacctgc acaggagtac ggtgattttt aattattgca
    14221 attgcacaag agtcagttct cccccaaaga cagcaccggt atcaatataa tgcaggttgc
    14281 caatatccac gcgatggcgc aaaggtgtat gaccaaacca gaaatgatca gcacctgcaa
    14341 ttccctgccc tttttggcgt tcacctaatc gcgagcggct ccacaagacc tgatgcaaat
    14401 cgacgtcctt ttgccattca tagacatcat ctggataatc ggcatgagca ataacatgtt
    14461 tgccggtgcg actgtgtaat tcaagaataa agggcaaatg ctgacatttt tccagcgccg
    14521 ttttcgcttg tttctgttga ttatctgcca gcgcaataaa ccagtcgccg ccatttatca
    14581 accataaaga catctgccgg gatgccagcg catccatcgc catctgttca tgattgcctc
    14641 ttaccgaacg aacccaatgt tgttccagta actgcaggca acgtaaactt tgcggcccac
    14701 gatcgataac gtctcccact gagataagta aatctcgcca cggatcaaaa cgacaatgcc
    14761 ataatttgcg gcgcaactgc tcaagacaac cgtgtatatc gcctgaaagc cagatatgtc
    14821 gccattgatg acccgcaatt ctctgataaa cgggcgcagg ctgtttcatc aatattttcc
    14881 tcccgcgcta aagatcacat aatcttaaca agaatgttaa aaaacgctgg actcagacag
    14941 tagagtgtgt gttatggttg actataaagt cagcgaaggg aatgcttctg gcttttaaca
    15001 gataaaaaga gaccgaacac gattcctgta ttcggtccag ggaaatggct cttgggagag
    15061 agccgtgcgc taaaagttgg cattaatgca ggcttagttg ccttgctctt taagaataga
    15121 tgacgacgcc aggttttcca gtttgcgtgc aaaatggtca ataaaaagcg tggtggtcat
    15181 cagcttaaat gttaaaaacc gcccgttctg gtgaaagaac tgaggcggtt ttttattgga
    15241 aatcaaaagg ctattttagg taattaacag agtttttcag ctcgctctat aaacggtgcc
    15301 agactcattt tttcgccggg attgttagga tcatcaatct gaatcaccga aatgggttgg
    15361 gctttagtct tcccactggc aacttccttt tgtgcgatat cgtttaaagg atactgcacg
    15421 agagtacttg ggttaatgac atacaaagca tcacctggtc ggcaagtcag catcacctct
    15481 tcgcgattaa acgcccattt gtctttaccc acttcaaaac ggctgacggt aatcacctgc
    15541 ggtgccgcca gcgcagctgc agaactggtg agtaacagaa acgccagaat actttttttc
    15601 atcattttcg taatccatca aaaaggttcg agagtcgcaa gcaggctgat gatcaccagc
    15661 actaccgcac ctatcgccca ttcgagttta gtcatccaga caaaccaggg agccgcacga
    15721 tcttcatcct gcctcatgcg cggtacaaga acataacgat tagccagcgc gatgaccacc
    15781 ataatcatca ccaggatggc tttgagcaat aagagttggc cccagtaagt cgtaagcgtg
    15841 gggggaaatc cggtaatcaa caatgcatta agcacgccgc ttaccagtac gccgatcacc
    15901 gcaaaatgcc cgcaccagga aaaacgcatc agcgcctgaa tagcctggtg tcgccaacgg
    15961 cctttgatga gttgcataca ccagagcacc ggcagtaatc ccccaaacca cgccgccgca
    16021 cagatcaggt gaattgcgtg attagtctga tggattttcg ccgttacccc ttcattcaac
    16081 gtcgcatgcc cgactcccgc cagcagaata aattgcgcgg tagtgagcat aaacagcaaa
    16141 cgtggcatat tccgcggttg cataagggcg acgatcaacg ttacgagggc gagaacaatt
    16201 tgccataacc agacgccacc aaactgcgtt tgcaaaacag ccaaccaaat gtttggcgaa
    16261 aatacatctg accatcccgc tcccatcaac ccgccctgaa cggcgagcat tgcagtggca
    16321 ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca gaaaacgctt cgtcagcaag
    16381 cgacgaatcg ttaagggggc cagccaggca ccgtacattg cgaagccaaa aaccagcatc
    16441 agcgaggtga aatggataaa tcgcagcgcg atccaggtaa acgccagcat gatttatttc
    16501 acactaaagg tgtaatgtcc tttcgtttta tgcccatcca ccgaaacaac atgccagtca
    16561 acggtgtagg ttccgggttt cagcgaatcg gccagtggaa caatcagttg cttttggtcc
    16621 tgctcattcc gcttcgccgg taatgtttta atattttcgt tttttggccc cgtgattttt
    16681 gcaccactga atcctgtttc aacgccttcc gagaagttta aagtgattgc ctgcggagct
    16741 gctgtcactt gtgcatttgc cgcaggatac tgatgcgtta aatgcgcatg tgcccagacg
    16801 gaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt agcgaaggga gcgtgcagtt
    16861 gaagccatat tatctattcc tttttgtaat aactttttta cagagcataa ccttgtctaa
    16921 tgtctgagtc gaggatcatc aattccggct tgccatcaag gctcactctt agtaactttt
    16981 gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct ggctaaactg gatcaaacag
    17041 aaatggataa agtgaatgtc gatttggcgg cggcaggggt ggcatttaaa gaacgctaca
    17101 atatgccagt gatcgctgaa gcggttgaac gtgaacagcc tgaacatttg cgcagctggt
    17161 ttcgcgagcg gcttatcgcc caccgtttgg cttcggtcaa tctgtcacgt ttaccttacg
    17221 agcccaaact taaataaaac ttatacatag ttacactttc ttacataacg cctgctaaat
    17281 tatgagtatt ttctaaaccg cccccataat ttgcagtcat tttgaaaagg aagtcattat
    17341 gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt aaaacctcac tcaccgaaca
    17401 ggtagctcat catctggagt ttgtgagggc cgccgccagg caacaatgtc agctgttagt
    17461 ttttccatcg ctttctttac tggggtgtga ttattcgcga cgtgcccttc ctgccccacc
    17521 cgatctctcg ctattagacc cgctttgtta tgccgcaacg acctggcgaa tgaccatcat
    17581 tgccggcctt cccgttgaat ataacgatcg ctttattcgt ggaattgcgg tattcgcacc
    17641 gtggcgaaaa acgccaggga tctaccatca gagttatggt gcatgtctgg gccgtcgttc
    17701 cagaaccatc acggtggtcg atgaacaacc gcaaggtatg gatatggacc cgacctgttc
    17761 actgtttacg acagggcaat gtctgggaga acccgacctg ctggcgtcgg cccgtcgctt
    17821 acaatttttt tcacatcagt actcgattgc tgtgctgatg gcaaatgccc gtggtaacag
    17881 tgcactatgg gatgaacatg gtcgtctcat cgttcgcgcc gatcgtggtt cattattgtt
    17941 agtcggtcag cgttcatcac agggttggca aggcgatatc attccattac gctaggcttt
    18001 ttcggcctgg agcatgccat gttgcgcatt atcgatacag aaacctgcgg tttgcaggga
    18061 gggatcgttg agattgcctc tgttgatgtc attgacggaa aaatcgtcaa tcccatgagc
    18121 cacctggtgc gtcccgatcg tcctattagt ccacaggcga tggcgattca tcgcatcacc
    18181 gaagccatgg tcgccgataa accgtggatt gaagatgtga tcccgcacta ttacggtagt
    18241 gaatggtatg tcgcgcataa cgccagcttt gaccgccgcg tactgcctga aatgcccggt
    18301 gagtggattt gcactatgaa actggcccgt cgtttgtggc cagggatcaa gtacagcaat
    18361 atggcgttat ataaaacacg gaagctcaat gtacagacgc cgccgggcct gcatcatcac
    18421 cgcgcgttgt atgactgtta tatcaccgct gcgttgctta tcgatattat gaacacctcc
    18481 ggctggacgg cagaacagat ggccgatatc accggacgtc catcgttgat gacgactttc
    18541 acctttggca aataccgtgg caaagcggtt tccgacgttg ccgaacgcga tccgggctat
    18601 ctgcgctggt tatttaataa cctggacagc atgagcccgg agctgcgttt aacactgaaa
    18661 cattatctgg aaaatactta atccgcaggc gtagcgggta atgttccctg cgccagcgcg
    18721 accagaaaag catattccat cgctacgcct tcgtacgatt taaagcgacc agatttaccg
    18781 ccatgacctg agtccatgtc ggtacagagc aataaaagat ggtcatcggt tttcagctcg
    18841 cgcaatttag cgacccattt tgccggttcc caatattgca cctgagaatc gtgtaaaccg
    18901 gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt tgtcatacgg gctgtagctt
    18961 ttcatatact cgtaatattg cggatcctgc ggattccccc actcttcaaa ttcgccggtg
    19021 gtaagaggaa ttgattcatc cagcattgtt gttacaacat caacaaacgg tacctgggcg
    19081 ataacgccgt ggaacaattc cgggcgttga ttaatcgcaa cgcccatcaa catacccccc
    19141 gcactcccgc ccatcgcata acagagcgaa ggagagccat agcccagttt taacaatgca
    19201 tcgcaggcat caagataatc attaaacgta tttttcttct tcagaaattt accgtcctcg
    19261 taccattgtt gccccagctc accgccgcca cgaacatgga caatggcgta gacaaatcca
    19321 cgatccaaca aactcaagcg gctaaaactg aaatcggcat caatacttgc gccgtaagaa
    19381 ccgtagccat acaccagcag cgggttgtgt cctttgcgaa aatgtttgcg atgatagacc
    19441 agcgaaaccg gaacttcgac gccatcacgg gcgactatcc acaggtgttc actgcggtaa
    19501 ttcgccgcat caaaaccagg aacttccgtt tgttttaata cacgacgctc accggtatcc
    19561 atatccagtt caaacaaagt atctggtgtg gtcatggaag aataaccata acgcaatcgc
    19621 gcggtttcag gttctggatt gtaggcaatc caggtcacat aggccggatc atcaaaggca
    19681 atgccaatga cttcccgggt cttgcggtta atttgccgca aactggttaa cccgcgctga
    19741 cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc cttccagcat aatgttatca
    19801 cgtggcggaa ttaactcttc ccactgttgc tcatcacgca tacgggtacg gtataagcca
    19861 aagtttttac cgtggcggtt ggaacgcaga taaaaccgat gctggtagtg atcaaggctg
    19921 tattcgtgat ctttgcggcg aggcagaaaa acaaacggct cggcatcggc catttccgcg
    19981 tccagcaggc gaacttcact ggtggtggcg ctggccagat gaatgaccac ataatgcttc
    20041 gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt tttcttcgta aatcagttta
    20101 tcttgcgatg ctggcgtacc gatggcgtga cgccagacct gataagggag cagcgtcact
    20161 ggatgcttgc gaacatagta gaaagtccag gagtcatttg cccagacaaa gctgggttca
    20221 acgttatcca gcagttccgg gtaccagtta cctgtttcca gattacgaaa acgaatgccg
    20281 tactgccgtc gggaaagaaa atcttctgcc agtgccataa tggtgttatc tggcgtaatc
    20341 gccattccgc ccatcgaata aaactcacta tgagccgcgc gcttgttggc atcgagcaat
    20401 gtttcccact cgtcccactc ttcactgaac gccgattgac gctggtagat agcatattca
    20461 cagcctggtt cataaatatg ccgatagcgg tagccatttt tgatgtaggg cgcagaaact
    20521 tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc gatcctgcaa ggcttgttgc
    20581 gaggccatca cccgatgacc gtaactattt tcttgttgca gatagtccag gacttccggc
    20641 tgagaacgcg tatcgtcccg cagccagtag taattatcga tgcgcgtatc gccatgaagc
    20701 gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt tattgttctt tctggttgaa
    20761 acatcttata agggtgacaa aactcaccgg ggatgcaagc gaaacagggt agtcattgct
    20821 tagatgatga caggtaatgg cgcggatatc gactgttatg cagacagaga aaatcagcct
    20881 gttcaaatgg ctgtgcggtt ctggataacc cgaaatagtc aacttcaggc tatccagaga
    20941 gcggaattac tccgccaaag tacgtttttg ctgttcgaga tcgcgttcaa tgccttcacg
    21001 aacgtcctgg gggattttca gcgcatcacc cagtgcattc aggtaactgc gttccataaa
    21061 atggtcaata tcaatagccg cgcagctcag gaaatagatt tccagcgcct cttcttcatt
    21121 gcggaccccg gtagccaggc gttgtggatc cagcggttgt tcgattgcct gctcaatgag
    21181 tacacgcccc ttctcttcca caccggcttc tcgcaattgc tggtcgattg ccgcacgttc
    21241 tttggcatcg atatggccat cacttttagc ggcaaagacc agcgcaagga tcaaacgttc
    21301 cgtacgctca tccagcggcg tactttgcgc gccaaactgc ggttcatcct gatgcgccgc
    21361 gcgaatttta tctttgtatt tattccacag caccgtaccc gctaccgctc cgccgccaac
    21421 cagtaacgcg tttgtgccat atttcgtaag aagtttacgt gctgatttat ttgcgaccag
    21481 cagcccagcc agtccgccta atgcgccagg cactaaaagt ttgcccaatc cctgatccgc
    21541 agacgaggag gtagaagaac ggctttgccc aagcagagat tgcagttgat ttaaccagtt
    21601 agccatattt gctcctcaat aaccattttt agtgcctgta gcatagcgga tgaggatgtc
    21661 agaaaatgta tatcgagaca aaagatgcgc aaataagcgg ggcggtaggc cgttattcaa
    21721 agaaattcgc gccaggtaag gtgttcactg tatataaggt gataagggta ttttaggtga
    21781 aagtagtttg acattttgag tggaactgtt tgatgctgat tgcccagttc gacgctactg
    21841 cgtcttctca ggactacgat tgtgttgcgt tgtagggcgg acaaggcact cgcgccgtat
    21901 ccgccaacgg tcaatcgaat tatcagaagc cattaacgcc gctgatattc cgccattccc
    21961 gctttgcagt ccacgctgac ctggtaatga atatcggcac ttttaccacg cacggttagc
    22021 ggtactgacc atttatcatc tttcccctga atgtcctgca aactgaccca tgccacgggg
    22081 tcggcctgac cgacaatttt ttgatcatca gcccagcgcg ccacgcgatt ttgttggtaa
    22141 tcacgtttta cgctcgcggc aattccggca gcatccagac cttcacactt tgggaaagtg
    22201 accgacttgc tggtttcatt attggcagcg aaaactgatg cgcaggcaga aaccaacaac
    22261 agccctaaaa acgcccctct ttttttcatg tttttctcca tagcacaatg attcaggaga
    22321 aagcatggta caaattgtca ggagcgcaag ttgcttcagg ccgcgtgtga ggcatcttcc
    22381 tcttcggatt cagcaccgac gacctgtggg gacggtaatt tgccggtttt cagaatggtg
    22441 ctcagcacat ctttttgttc tgccagccat agcgaaaggg cttcacgttg ctcatcttcc
    22501 atttgcactg gagaatttgt cagccaggtg tcaagcagat cggccgtatc aagcatcttg
    22561 tcataagcat cggcttcctt tttgctggta aacgacattt tctcttcgcc ctcacgaatg
    22621 actacgtatt taacttcaac cgccatttgc agcctctcat aataactgtg attttataca
    22681 gtatatttct tttcagttgg gaaatcaaca tcagcaataa agacacacgc aaacgttttc
    22741 gtttatactg cgcgcggaat taatcagggg atattcgtta tgacgttatt aggcactgcg
    22801 ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg gtgaactggg taaagaagtg
    22861 gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg tcgatcgcta tgccgacgca
    22921 ccagccatgc atgtcgcgca tcgctcccat gtcattaata tgcttgatgg tgatgcatta
    22981 cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc cggagatcga agctattgcc
    23041 accgatatgc tgatccaact tgaagaggaa ggactgaatg ttgtcccctg cgctcgcgca
    23101 acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg cggcagaaga gctgcagctg
    23161 cccacttcca cttatcgttt tgccgatagc gaaaaccttt tccgcgaagc ggttgccgcc
    23221 attggctacc cctgtattgt taaaccggtg atgagttctt ccggtaaagg gcagacgttt
    23281 attcgttctg cagagcaact tgctcatgca tgggagtacg ctcagcaagg tggtcgcgcc
    23341 ggagcgggcc gcgtaattgt tgaaggcgtc gttaagtttg acttcgaaat taccctgcta
    23401 accgtcagcg cggtggatgg cgtccatttc tgtgcaccgg taggtcatcg ccaggaagat
    23461 ggcgactatc gtgaatcctg gcaaccacag caaatgagcc tgcttgccct tgaacgtgcg
    23521 caggagattg cccgtaaagt ggtgctggca ctgggcggtt atgggctgtt tggtgtcgag
    23581 ctgtttgtct gtggtgatga ggtgattttc agtgaagtct cccctcgccc gcatgacacc
    23641 gggatggtga cgctaatttc tcaggatctc tcggagtttg ccctgcatgt acgtgccttc
    23701 ctcggacttc cggttggcgg gatccgccag tatggtcctg ctgcttctgc cgttatcctg
    23761 ccgcaactga ccagtcagaa tgtcacgttt gataatgtgc agaatgccgt aggcgcagat
    23821 ttgcagattc gtttatttgg taagccggaa attgatggca gccgtcgtct gggggtagca
    23881 ctggctactg cagagagtgt tgttgacgcc attgaacgcg cgaagcacgc cgccggacag
    23941 gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa aaatgcccga tcctcgatcg
    24001 ggcattttga cttttacagc ttagcgcctt ctacagcttc acgcgccagc ttagtaatgc
    24061 ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag ccaggaacca ccgatgcaca
    24121 gcacgctttt cagcgccagg tagtcacggt agttagccgg agaaatacca cctgtcgggc
    24181 agaaacggac ctgagagaac ggacccgcga tcgcctgcag ggctttcaca ccgccgttag
    24241 cttcagccgg gaagaatttg aactctttca aaccgtagtc catacccagc atcagttcgg
    24301 aaacagtgct gatccccgga atcagaggaa tagtcccttc ggtagcagct ttcagcagcg
    24361 gctcggtcag acccgggcta attgcaaact gtgcacctgc ttcagtgact tctgccagct
    24421 gctgtggatt cagcaccgta ccggcaccca caatcgcttc aggcacttct ttggcgatag
    24481 cacggatagc gtcaactgcg cactcggtac gcagagtcac ttccagaacg cgcaccccac
    24541 cagcaaccaa cgcttttgcc atcggcaccg cgtgttccag tttttttacc acgataaccg
    24601 gtacaaccgg gccggtggtc aggattgatt ctgcacttgt tttccagttt ttcatcagag
    24661 ttttctctcg cctgattaca aatttgtcgt cttaaaaagt gatacaggtt gcgccctgtt
    24721 cggcaccgga cagtttttca cgcaaggcgc tgaataattc acgtcctgtt cccacgcgtg
    24781 acgcgctcag gtcaggaatg tgcggttcgc gagcagccag ttccgcttcg tctaccagca
    24841 gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc cccgtcgcgc acttttgcca
    24901 gcagcccgcc atcgtaggct tctggtgtta cgtggatagc tgacggcact ttacctgaag
    24961 cgccggagag tcgtccatcg gtaactaacg caattttgaa acaccggtcc aataatacac
    25021 caagtggcgg catgagttta tgtaattctg gcattccgtt cgcttttggc ccctgatgac
    25081 ggacaacaac gacacaatcg cggtccagta aacccgcttc aaaggccggc ataacgtcat
    25141 gctggctttc aaaaacaacc gctggcgctt caatcacctg gttctctacc ggcacggcag
    25201 aggttttcat aaccgctcgg cccaggttac cgcttaacac ttttgtccca ccatgatgag
    25261 agaaaggttg ttcgaaggac gcgatcacat tgttgtcgag tgatttttct gccccttccc
    25321 gccagtccag ttcaccatta ttcagccatg gttcaagggt ataacgagac agaccaaagc
    25381 ctgccaccgt attgacatct tcatgcagca ggcctgcttt gagcagttca cgtaccagaa
    25441 ccggtacgcc acctgccgcc tggaagtggt taatatcggc cggaccgttc gggtagagac
    25501 gtgccatcag cggtacaaca tcagaaaggt cagagaagtc atcccagtta atctgaatac
    25561 cggctgcgcg cgccatcgcc accaggtgca tggtgtggtt agtggaacca ccggtcgcca
    25621 gcagtgcaac gataccgttc accaccactt tctcatcgat catcttaccg atcggcatcc
    25681 attcattacc gttgccagtc atgcgtgtaa cctggcgcgc agctgcagcg gtcaaagcat
    25741 cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg caactgcatc cccataaact
    25801 ccaccaccat ctggttggtg ttggcagtac cgtagaaagt acatgttccc ggcgcatgat
    25861 aagacgcggc ttctgactcc agtaatgcca tgcggtccac tttaccttcg gcataaagct
    25921 ggcgaatacg cactttttct ttatttggca aaccgcttgc catcggtcca gacggcacaa
    25981 acaccgcagg caaatgacca aacgacaggg ctgccatggt cagacccggg acaatcttgt
    26041 cgcacacacc gagaaacaga gcaccatcaa acatgttatg ggacagcccc accgccgcag
    26101 acatcgcaat cacttcgcgg cttaacagcg acaattccat tccatcctgc ccctgggtga
    26161 caccatcaca catcgccgga acaccgcccg caacctgacc aaccgcattc gcttcatgca
    26221 gggctttacg aatgatttcc ggatagtgtt cataaggctg gtgagcggag agcatgtcgt
    26281 tataggaggt gatgatggcg atattgttac gcaacatgct tttcaaagag gctttgtctt
    26341 ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca tgccaactgc gaacgatgaa
    26401 cggtcgaagt tttcgcttgt tctatccggg cgaggtaagc agagcgagtc tcgcgcgaac
    26461 gttcaatgat tcgatttgtt acgcgtaaca attgtggatt cataaaggct cctgaaattg
    26521 agttgtcaga gcaggatgat tcacaacacg tttcattcag aggattcatg actgaaacgc
    26581 ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt gtaataaaaa aagcctcgtg
    26641 ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct acaatctgtg caagatcatg
    26701 ttaccggtaa aataaccata aaggataagc ccagatatta ctcaaactca ttccaggaac
    26761 gaccgtcacg ggtaatcatc gccaccgagg caacggggcc ccaggttccg gcctgatacg
    26821 gtttcggcgc atcattgtcc atcgcccacg cctcagtaat ggagtctacc catttccagg
    26881 cttcttccac ttcatcacga cgtacaaaca gtgcctgaat accacgcatg gtttccagca
    26941 gcagacgttc ataggcatct gccagatgcg tttgattaaa ggtttctgaa tagctcagat
    27001 ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc aggaacttta ttcagtacct
    27061 ggatatccac gccttcatca ggttgcagac ggatagtcag tttattctgc ggcagatcct
    27121 gccacgactc tttaaacaga ttcagttcag gtgttttgaa atagaccacg acttcagaac
    27181 atttggtcgg cagacgttta ccagtacgca ggtagaatgg cacaccggcc cagcgccagt
    27241 tatcaatgtc gacgcggatc gccacgaagg tttctgtatt gctgctcttg ttcgcgccct
    27301 cttcttccag atagcccggc acttttttgc cctgagcgaa gcccgcagta tattgcccgc
    27361 gtacggtttt ttcgcgcacg ttggagcggt cgatgcggcg cagagacttc agtactttca
    27421 ctttttcatc gcggatgctg tctgcgctca ggtcagacgg cggagacatc gcaatcatgc
    27481 aaagaatttg cagcaggtgg ttctggatca tgtcgcgcat ctgaccggct ttatcaaaat
    27541 agccccagcg cccttcgatc cccacttctt ctgccacggt aatctcaaca tgatcaatgg
    27601 tgcgattgtc ccagttgttc acaaacagag agttagcaaa acgcagcgcc aacaggttca
    27661 gcactgtttc tttaccaaga tagtggtcga tacggtaaac ctggcactcc tcgaagtatt
    27721 cgccaacctg atcgttgatt tcctgcgagg tcgccagcga cgtccccagc ggtttttcca
    27781 tgactacgcg tgccggttta gcattcagtt ttgcctcacc aagccctttg caaattgcgc
    27841 caaaagtgct gggcggcatg gcaaagtagt taatggtgat acgatttttt tgatccagca
    27901 tcgcgccgag acggctgaat gcagcagtgt cattgacatc aagattacaa aaatccagac
    27961 gtgcactcag ggtgtcccat aaaccttcat caatggtttc tttcatgaaa gtttcgagcg
    28021 cctcgcggac aactttggta taagccgctt tatcccagtc agcacgccct acgccgataa
    28081 tccgggtgtc cgggttgagc tgaccggctt tttccagttg atacagggaa ggcagcaatt
    28141 tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag gtcacaggcc tgggctgttt
    28201 gcgttaccgc catgtcattc tccttaagtt aactaacccg gtacttaagc cagggtatac
    28261 ttgtaatttt cttacggtgc actgtactgc ttttacgagc ttgcgaaaac tgtaaacgct
    28321 tatccacccg tgcgattacg gtaaaagcgc gcaaagtgcg gcaaaactga taaaaaaatc
    28381 atcgttttcc attgggtaaa aatctgacac tgatcatgtt atgaaaaaaa ataacaactt
    28441 ttttatctgc ttttgtcatt aacacagcac acaggcgtaa tatctgacaa aactgcattt
    28501 cgatttcttt cagagcggaa atcgtcatta cccgtgagtc tctttacatc atgaatatgc
    28561 tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc tgagcgcaaa gttgccgagg
    28621 tcattctggc ttcgcccgat aacgcgatcc attcgagtat tgctgctctg gcgctggaag
    28681 ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag catggacaca cgcggttttc
    28741 ctgattttaa acttcatctg gcacaaagtc tggcgaatgg cactccctat gttaatcgca
    28801 acgtcaatga agatgacagc gttgaatcat acacggggaa aatatttgag tccgcaatgg
    28861 caacgctcga tcatgtccgt cattcactgg ataaatctgc catcaaccgc gccgtcgact
    28921 tgctcactca ggcaaaaaaa atcgcctttt tcggattagg ttcttcagcc gccgttgccc
    28981 acgatgcgat gaataagttc tttcgtttta atgttccggt ggtgtactcc gatgatatcg
    29041 tgctgcaacg catgagttgt atgaattgta gcgacggaga cgtggtggtg ctgatttctc
    29101 acacgggaag aacaaaaaat ctggtcgagc tggcgcagct ggcacgcgaa aacgacgcta
    29161 tggtaattgc cctcacctct gcgggcaccc cactcgcccg ggaagcaacg ctggcaatta
    29221 ccctcgacgt accggaagat actgacattt atatgcccat ggtttctcga cttgcacagc
    29281 tgaccgtgat agatgtgctg gcgacaggat ttactttgcg gcgcggtaca aaattcagag
    29341 ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg ttttgataag cagttactta
    29401 atttaagtga cgatcgctaa aaacgactgt cactgtccta atcttatacg acatccgaat
    29461 gagattaatt tatcgccatc acggcgttat ttcattcgga tttcatgttc aagcaacacc
    29521 tggttgtttc agtcaacgga gtattacatg tccagaaggc ttcgcagaac taaaatcgtt
    29581 accacgttag gcccggcaac agatcgcgat aataaccttg aaaaagttat cgcggcgggt
    29641 gccaacgttg tacgtatgaa tttttctcac ggctcgcctg aagatcacaa aatgcgcgcg
    29701 gataaagttc gtgagattgc cgcaaaactg gggcgtcatg tggctattct gggtgacctc
    29761 caggggccca aaatccgtgt atccaccttt aaagaaggca aagttttcct caatattggg
    29821 gataaattcc tgctcgacgc caacctgggt aaaggtgaag gcgacaaaga aaaagtcggt
    29881 atcgactaca aaggcctgcc tgctgacgtt gtgcctggtg acatcctgct gctggacgat
    29941 ggtcgcgtcc agttaaaagt actggaagtc cagggcatga aagtgttcac cgaagtgacc
    30001 gtcggtggtc ccctctccaa caataaaggt atcaacaaac ttggcggcgg tttgtcagct
    30061 gaagcgctga ccgaaaaaga caaagcagac attaagactg cggcgttgat tggcgtagat
    30121 tacctggctg tctccttccc acgctgcggc gaagatctga actatgcccg tcgtctggca
    30181 cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg aacgtgcgga agccgtttgc
    30241 agccaggagg caatggatga catcatcctc gcctctgacg tggtaatggt tgctcgcggc
    30301 gacctcggtg tggaaattgg cgatccggaa ctggtcggca ttcagaaagc gttgatccgt
    30361 cgtgcgcgtc agcttaaccg cgcagtaatc actgcgaccc agatgatgga gtcaatgatt
    30421 actaacccga tgccgacgcg tgcagaagtc atggacgtag caaacgccgt tctggatggt
    30481 actgacgctg tgatgctgtc ggcagaaact gctgctggtc aatacccgtc agaaactgtt
    30541 gcagccatgg cgcgcgtttg cctgggggcg gaaaaaatcc cgagcatcaa cgtttctaaa
    30601 caccgtctgg atgttcagtt cgacaatgtg gaagaagcca ttgccatgtc agcaatgtac
    30661 gcggctaacc acctgaaagg cgttacggcg atcatcacca tgaccgaatc gggtcgtacc
    30721 gcgctgatga cctcccgtat cagctctggt ctgccaattt tcgccatgtc acgccatgaa
    30781 cgtacgctga acctgactgc tctctatcgc ggcgttacgc cggtgcactt cgatagcgct
    30841 aatgacggtg tagcagctgc cagcgaagcg gttaatctgc tgcgtgataa aggttacttg
    30901 atgtctggtg acctggtgat tgtcacccag ggcgacgtga tgagtaccgt gggttctact
    30961 aataccacgc gtattttaac ggtagagtaa gtacgttgcc ggatgcggcg aaaacgcctc
    31021 atccggccta cagttcaatg ataattcaac agatttcgaa tcttctgaag caaacttgaa
    31081 cttatcatca gtcgaaggcc tctcctcgcg agaggcactt tttatttgat gggataaaga
    31141 tctttgcgct tatacggttg gatttcgccc ggtttgcgag ttttcagcaa ttttaatatc
    31201 caggtgtatt gttctggtcg cggaccaaca aaaatctcga cttcttcatt catccgccgc
    31261 gcaatcgtat gatcatccgc ctctaacaga tcatccatcg gtgggcgcac ctgaatcgtc
    31321 agacgatgcg tcttgccatc ataaatcgga aacagcggta caacgcgcgc acggcacact
    31381 ttcatcaaac gaccaatcgc gggcaacgtc gctttatagg tggcaaagaa atcaacaaat
    31441 tcgctgtgtt ctgggccatg atcctgatcg ggtaaataat atccccagta accctgacgt
    31501 accgactgga tgaatggttt aataccatca tttctcgcat gcagacgacc accaaagcga
    31561 cggcgcaccg tgttccagac ataatcaaaa accgggttgc cctgattatg gaacatcgct
    31621 gccattttct gcccttgcga ggccatcagc atggcaggaa tatcgacggc ccaaccgtgc
    31681 ggcaccagaa aaatcacttt ctcgttatta cgccgcatct cttcgatgat ctccagccct
    31741 tgccagtcaa cgcgcggctg aattttctcc ggcccgcgta ttgccaactc agccatcatt
    31801 accatcgctt gcggcgcggt ggcaaacatc tcatctacaa tcgcttcgcg ttcagcttca
    31861 ctacgttctg gaaagcagag cgacagattg attaacgcac gacggcgtga gctttttccc
    31921 agtcgtccgg caaaacgtcc cagccgtgcc agaatgggat cacggaactt tggcggcgtt
    31981 aaagcgatac ccgccatcgc tgctacgccc agccatgctc cccagtagcg cgggtggcga
    32041 aaggatttat caaactcagg aatgtattcg ctattatttt ttttcgtttc catgcttttc
    32101 cagtttcgga taaggcaaaa atcaatctgg tgatagtgta gcggcgcaac ttgccccgca
    32161 ccaaataaaa aagccggtac tgactgcgta ccggctgcga atggatgtta attaatcaaa
    32221 ccgtagctgc ggcacaatct ctttggcctg tgccaggaat tcgcgacgat cggagccggt
    32281 cagcccttcg gtacgcggca gttttgccgt cagcgggttt acggcctgct ggtttatcca
    32341 tacttcatag tgcagatgcg gcccggttga acgtccggta ttaccggaaa gcgcgatacg
    32401 gtcgccacgt ttcaccttct gtcccggttt caccaggatc ttgcgcaagt gcatataacg
    32461 cgtggtgtag ctgcgaccat gacgaatagc cacataataa cctgctgcgc cactgcgttt
    32521 ggcaaccacc acttcaccgt cacccactga aagcactggc gtaccttgtg gcatggcaaa
    32581 atcaacacct ctgtgtggcg caacgcgacc ggtcaccgga ttagtacgac gcgggttaaa
    32641 gttagatgag atacggaact gtttcgccgt cgggaatcgc aagaatcctt tcgccagacc
    32701 agtaccgtta cgatcgtaga atttgccatc ttcagcgcgg attgcgtaat aatctttacc
    32761 ttctgaacgc aaacgtacgc ccagcagctg gctttgctca cgtttaccat caagcatttc
    32821 tcgtgacatt aacaccgcaa attcatcgcc ttttttcagt ttgcggaaat ccatttgcca
    32881 ctgcatggct ttaatcactg cgctcacttc ggcgctggtt aaaccggcgt ttctggcgct
    32941 ggcaacaaag cttcccccga cggtaccttt cagcagattg ttgacccact ctccttgctg
    33001 catttcgctg gtcattttaa aaccgttagc ggcagtacgg tcataggttc gggtttcacg
    33061 acgagacact tcccaggtga ggcgctgcag ttcgccgtcc gcggttaatg tccaggagag
    33121 ttgttgaccg attttcaggt tacgcaattc tttgtcggca gcagccagtt gggtgatatc
    33181 acccatatca ataccatact gattgagaat gctgcttagc gtatcgccag tggaaacaac
    33241 atattcatgc acgcccgctt caccggcgat tttgtcatcc agttcgtcct ggggaatggc
    33301 ttcatcttct tgtgcagctt gatcaatcgg ctcactggct tcaggtaaga gcgaacgaat
    33361 ttcgttctgt tccagctcaa tggttttgac aattggcgtg gcatcgcggt gataaacata
    33421 gggccgccag acagcgacgg ccagagtaag aacggtgagc gaccccaaca taacgcggtg
    33481 tggtcgcggt aaattattaa acgccagggc gacagagcgg gctatctgtt gcacgtaatc
    33541 acttcctcat taatctcctt tcaggcagct cgcatactgg ttggctaatt gattcaggaa
    33601 ttctgaatag cttgttttac ccagtttgat attcgtcccc aggggatcca acgttcccat
    33661 acgaacggat gtccctcgtg cgacgctctc aacgaccgct ggcctgaact gtggctcagc
    33721 aaaaacgcag gttgcttttt gctcaaccaa ctgtgttctt atttcatgta aacgctgcgc
    33781 gccaggttga atctcagggt ttacggtaaa atgaccaagc ggtgtcagtc cgaactgttt
    33841 ttcgaagtag ccgtaagcat cgtgaaaaac gaaataacct ttccccttga gcggcgcgag
    33901 ctcgttacca acctgcgttt cggttgaggc taattgtgcc tcaaaatcct tcaggttggc
    33961 gtcaagtttg gctcgacttt gcggcataag ttccactaat tttccatgga ttgcaaccgc
    34021 tgtagcccgc gctatctctg gggaaagcca aagatgcatg ttgaaatcgc cgtgatggtg
    34081 atcttcgtca cttttttccg cgtgatcgtg atcatcatca tcgtcgtgaa tacttttcat
    34141 cagcagcggt ttcacattct ctagctgcgc aatcgttacc tgtttcgctt ctggtaattt
    34201 acttaccggt ttttgcataa acgcttccat ctccgggcca acccaaacga ctaagtccgc
    34261 gttctgtaag cgttttacat ccgatggacg cagtgaataa tcatgttctg aagcgccgtc
    34321 aggcagtaaa acctgcgttt ctgttacccc atcagcaatg gcagaagcga tgaacccaac
    34381 gggtttaagc gaagcgacaa cggcggcatc tgcggcctgt gttgcaccgc cccagagagc
    34441 ggcggataat gctgcgaaaa gaagcgtttt tttatgtaac ataatgcgac caatcatcgt
    34501 aatgaatatg agaagtgtga tattataaca tttcatgact actgcaagac taaaattaac
    34561 atgacaagtc tggtttccct ggaaaatgtc tcggtttctt ttggccaacg ccgcgtcctc
    34621 tctgatgtgt cgctggaact taaacctgga aaaattttga ctttacttgg gccaaacggc
    34681 gcaggtaagt cgacactggt acgggtagtg ctcggactgg taacacccga tgaaggggtt
    34741 atcaagcgca acggaaaact gcgcatcggc tatgtaccgc agaagctgta tctcgacacc
    34801 acgttgccgc tgaccgtaaa ccgtttttta cgcttacgcc ctggcacaca taaagaagat
    34861 attttgcctg cactgaaacg tgtccaggcc gggcatctga ttaacgcacc gatgcaaaag
    34921 ctctcgggtg gcgaaacgca gcgtgtactg ttagcgcgag cattgttaaa tcgaccgcaa
    34981 ttattagtgc tggatgaacc cactcagggc gtggatgtga atggtcaggt ggcgttatat
    35041 gaccttattg accaactgcg tcgcgagctg gattgtggcg ttttaatggt atctcacgat
    35101 ctgcatctgg taatggcaaa aaccgatgaa gtgctttgcc tgaatcacca catttgttgt
    35161 tccggcacac cggaagttgt ttccctgcat ccggagttta tttctatgtt tggtcctcgt
    35221 ggtgctgaac aactgggtat ctatcgccat catcataatc atcgtcacga tttacaggga
    35281 cgaattgttt tgcgtcgggg aaatgatcgc tcatgattga attattattt cccggttggt
    35341 tagccgggat catgctcgcc tgtgccgcgg gtccgctggg ttcgtttgta gtctggcgtc
    35401 gtatgtctta tttcggtgat acgctggctc atgcctcatt acttggcgtc gcgtttggtt
    35461 tgttgctgga cgtgaatcca ttctatgcgg tgattgcagt tacgctgctg ctggcgggcg
    35521 gtctggtatg gctggagaag cgtccacagc tggcgatcga cacgttatta gggattatgg
    35581 cgcacagtgc cctgtcgctg ggcctggtgg tcgttagtct gatgtctaat attcgtgttg
    35641 atttgatggc ttacctgttc ggtgatttac tggcagtgac gccagaagat ctcatctcta
    35701 ttgcgattgg cgtggtcatc gtggtggcta ttttgttctg gcaatggcgc aatttgctgt
    35761 cgatgacgat tagcccggat ctggcgtttg ttgatggtgt gaaattacag cgcgtgaaat
    35821 tgttgttgat gctggtgacg gcattgacga ttggtgtagc gatgaaattc gtcggcgcgt
    35881 tgattattac ttcactgctg attattcctg ctgctactgc acgtcgcttt gcccgcacgc
    35941 cggaacagat ggctggtgtc gctgttttgg tggggatggt ggcagtgact ggcggtttaa
    36001 ccttttccgc attttacgat acacctgcag gcccgtcggt ggtgctatgc gcggcactgt
    36061 tatttattct cagtatgatg aaaaaacagg ccagctaatc tttcgctgca catatttgtc
    36121 ggatgcggcg cgagcgcctt atccgaccta cggttcgcta tctctggtag gcctgataag
    36181 acgaacagcg tcgcatcagg cacactgcca gtgtcggatg cggcgcgagc gaccaatccg
    36241 acttacggca tttctggcgg cgttatgcca aagtgattcc acgcccgcgt cgtcgccata
    36301 cgcccacgcg gtgtacgctg caaaaagcct tgctgaatca aataaggttc cagtacatcc
    36361 tcaatggttt cacgttcttc gccaatggct gccgccaagt tatccagacc taccggccca
    36421 ccaaagaact tatcgattac cgccagcaac aatttgcggt ccatataatc gaaaccttca
    36481 gcatcgacat tcaacatatc cagcgcctga gcagcgatat ctgccgagat ggtgccatcg
    36541 tgcttcactt cggcgaaatc acgcactcga cgcagcagac ggttggcaat acgtggcgta
    36601 ccgcgcgcac gacgagcaac ttccagcgcg ccgtcatcac tcatctcaag ccccataaag
    36661 cgtgcgctgc gactgacgat atattgcaga tccggcacct gataaaactc cagacgttgc
    36721 acaataccaa aacgatcgcg caacggtgat gtcagcgaac ctgcgcgcgt ggttgcacca
    36781 atcagggtaa acggcggcaa atcaatttta atggagcgtg ccgccggacc ttcaccaatc
    36841 atgatatcca gttggtaatc ttccattgcc ggatacaaca cctcttccac cactggtgaa
    36901 agacggtgga tctcatcaat aaacagtaca tcgtgtggtt caaggttagt gagcattgct
    36961 gccagatcgc ccgccttttc cagcaccgga ccagaagtcg tgcgtaaatt aacgcccatt
    37021 tcattggcga caatattggc aagcgtagtt ttacccaacc ccggaggacc aaaaatcaat
    37081 agatgatcga gggcatcgcc gcgcagtttc gctgctttga tgaaaatctc catctgcgaa
    37141 cgaacctgcg gctgaccaac atactcttcc agtaatttag ggcgaatggc gcgatctgcc
    37201 acatcttccg gcaaagtggt accggcagaa atcagacggt ctgcttcaat catcctttac
    37261 ctcataacgc ggcgcgtagg gcttcgcgaa ttaatgtttc actgctggcg tcagggcgag
    37321 cgattttgct caccatgcgg cttgcttctt gtggtttata gcccagtgcc accagcgcag
    37381 caaccgcttc ctgttcagca tcgtcggtcg ccgggctggc aggagacgtg agtaccaggt
    37441 cggcggctgg cgtaaagaga tcgccatgca aacctttaaa tcggtctttc atttcgacaa
    37501 tcaagcgttc ggcggttttt ttgccaatac ccggcagttt caccagtgcc cccacttctt
    37561 cacgctcaac ggcattaacg aactgctgcg ctgacattcc ggagaggatc gccagcgcca
    37621 acttcgggcc gacgccgttg gttttgatca actctttgaa caacgtgcgc tcttgtttat
    37681 tgttaaaacc gtacagcagt tgcgcgtctt cacgcaccac aaagtgggtg aaaacgatcg
    37741 cttcctgacc cgcttcaggg agttcataaa aacaggtcat cggcatatgc acttcatagc
    37801 ctacgccgcc cacttcaatt aacaccagcg ggggttgttt ttcaatgatg atgcctctga
    37861 gtctgcctat cacatgacgc tcctgcgtaa tgaatcaaag ataatgctgt atgataaaaa
    37921 aatgctggat agatatccag cgaaggatga agaaaacttg ctaggtgtct cga
//
LOCUS       DAZFJL010000027        37943 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFJL010000027 DAZFJL010000000
VERSION     DAZFJL010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.027"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(32..2008)
                     /gene="speA"
                     /locus_tag="ACHJ8C_001400"
     CDS             complement(32..2008)
                     /gene="speA"
                     /locus_tag="ACHJ8C_001400"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO5551285.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2017..2148)
                     /gene="yqgB"
                     /locus_tag="ACHJ8C_001401"
     CDS             complement(2017..2148)
                     /gene="yqgB"
                     /locus_tag="ACHJ8C_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO5551286.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(2242..2385)
                     /gene="yqgG"
                     /locus_tag="ACHJ8C_001402"
     CDS             complement(2242..2385)
                     /gene="yqgG"
                     /locus_tag="ACHJ8C_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO5551287.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2496..2747)
                     /gene="yqgD"
                     /locus_tag="ACHJ8C_001403"
     CDS             complement(2496..2747)
                     /gene="yqgD"
                     /locus_tag="ACHJ8C_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO5551288.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTQFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2803..3957
                     /gene="metK"
                     /locus_tag="ACHJ8C_001404"
     CDS             2803..3957
                     /gene="metK"
                     /locus_tag="ACHJ8C_001404"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO5551289.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4394..5788
                     /gene="galP"
                     /locus_tag="ACHJ8C_001405"
     CDS             4394..5788
                     /gene="galP"
                     /locus_tag="ACHJ8C_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO5551290.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQIMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5865..6362
                     /gene="yggI"
                     /locus_tag="ACHJ8C_001406"
     CDS             5865..6362
                     /gene="yggI"
                     /locus_tag="ACHJ8C_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO5551291.1"
                     /translation="MKTSRLPIALQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCIHCGE
                     QLVAK"
     gene            6457..7164
                     /gene="endA"
                     /locus_tag="ACHJ8C_001407"
     CDS             6457..7164
                     /gene="endA"
                     /locus_tag="ACHJ8C_001407"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO5551292.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     VAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7244..7975
                     /gene="rsmE"
                     /locus_tag="ACHJ8C_001408"
     CDS             7244..7975
                     /gene="rsmE"
                     /locus_tag="ACHJ8C_001408"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO5551293.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            7988..8938
                     /gene="gshB"
                     /locus_tag="ACHJ8C_001409"
     CDS             7988..8938
                     /gene="gshB"
                     /locus_tag="ACHJ8C_001409"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO5551294.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMADLY
                     LINGEARARTRTLSVEQNYDKWYEFTGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEDKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9047..9610
                     /gene="yqgE"
                     /locus_tag="ACHJ8C_001410"
     CDS             9047..9610
                     /gene="yqgE"
                     /locus_tag="ACHJ8C_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO5551295.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9610..10026
                     /gene="ruvX"
                     /locus_tag="ACHJ8C_001411"
     CDS             9610..10026
                     /gene="ruvX"
                     /locus_tag="ACHJ8C_001411"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO5551296.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10200..11180)
                     /gene="yggR"
                     /locus_tag="ACHJ8C_001412"
     CDS             complement(10200..11180)
                     /gene="yggR"
                     /locus_tag="ACHJ8C_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO5551297.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVKEGRL"
     gene            11198..11902
                     /gene="yggS"
                     /locus_tag="ACHJ8C_001413"
     CDS             11198..11902
                     /gene="yggS"
                     /locus_tag="ACHJ8C_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO5551298.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11920..12486
                     /gene="yggT"
                     /locus_tag="ACHJ8C_001414"
     CDS             11920..12486
                     /gene="yggT"
                     /locus_tag="ACHJ8C_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO5551299.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            12483..12773
                     /gene="yggU"
                     /locus_tag="ACHJ8C_001415"
     CDS             12483..12773
                     /gene="yggU"
                     /locus_tag="ACHJ8C_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO5551300.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12781..13374
                     /gene="rdgB"
                     /locus_tag="ACHJ8C_001416"
     CDS             12781..13374
                     /gene="rdgB"
                     /locus_tag="ACHJ8C_001416"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO5551301.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13367..14503
                     /gene="hemW"
                     /locus_tag="ACHJ8C_001417"
     CDS             13367..14503
                     /gene="hemW"
                     /locus_tag="ACHJ8C_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO5551302.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAANLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEPAPRVEFSQYTGLS
                     EEVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(14568..15575)
                     /gene="yggM"
                     /locus_tag="ACHJ8C_001418"
     CDS             complement(14568..15575)
                     /gene="yggM"
                     /locus_tag="ACHJ8C_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO5551303.1"
                     /translation="MKKQWIVGTALFMLMTGNVRADGEPPTENILKDQFKKQYHGILK
                     LDVITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQNNSIPALEKDVKALDKQMVAAQKAADAYWVKDANGKQMTREDAFKKIHQQRDDFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKIS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVTILMKIDDINQVNERWKKDTEQLRRNGVI
                     K"
     gene            complement(15692..16738)
                     /gene="ansB"
                     /locus_tag="ACHJ8C_001419"
     CDS             complement(15692..16738)
                     /gene="ansB"
                     /locus_tag="ACHJ8C_001419"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO5551304.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPVRK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(16914..17633)
                     /gene="yggN"
                     /locus_tag="ACHJ8C_001420"
     CDS             complement(16914..17633)
                     /gene="yggN"
                     /locus_tag="ACHJ8C_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO5551305.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(17817..18143)
                     /gene="yggL"
                     /locus_tag="ACHJ8C_001421"
     CDS             complement(17817..18143)
                     /gene="yggL"
                     /locus_tag="ACHJ8C_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO5551306.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLEEVRTSEL
                     FDVWWD"
     gene            complement(18143..18862)
                     /gene="trmB"
                     /locus_tag="ACHJ8C_001422"
     CDS             complement(18143..18862)
                     /gene="trmB"
                     /locus_tag="ACHJ8C_001422"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO5551307.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLSSAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGIFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            19023..20075
                     /gene="mutY"
                     /locus_tag="ACHJ8C_001423"
     CDS             19023..20075
                     /gene="mutY"
                     /locus_tag="ACHJ8C_001423"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO5551308.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVAALHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEDNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPA"
     gene            20103..20378
                     /gene="yggX"
                     /locus_tag="ACHJ8C_001424"
     CDS             20103..20378
                     /gene="yggX"
                     /locus_tag="ACHJ8C_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO5551309.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            20443..21522
                     /gene="mltC"
                     /locus_tag="ACHJ8C_001425"
     CDS             20443..21522
                     /gene="mltC"
                     /locus_tag="ACHJ8C_001425"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO5551310.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNLTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            21724..22980
                     /gene="nupG"
                     /locus_tag="ACHJ8C_001426"
     CDS             21724..22980
                     /gene="nupG"
                     /locus_tag="ACHJ8C_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO5551311.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAEVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAVLVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(23029..25164)
                     /gene="speC"
                     /locus_tag="ACHJ8C_001427"
     CDS             complement(23029..25164)
                     /gene="speC"
                     /locus_tag="ACHJ8C_001427"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO5551312.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVALGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(25327..25410)
                     /locus_tag="ACHJ8C_001428"
     CDS             complement(25327..25410)
                     /locus_tag="ACHJ8C_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5551313.1"
                     /translation="MHATSLHSLAYRRFARNSSLFMLLLMR"
     gene            25563..26270
                     /gene="yqgA"
                     /locus_tag="ACHJ8C_001429"
     CDS             25563..26270
                     /gene="yqgA"
                     /locus_tag="ACHJ8C_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO5551314.1"
                     /translation="MVIGPFINASAVLLGGVFGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTVVAKAHNLFHHSRKKPAHESF
                     IQNFVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            26376..26451
                     /locus_tag="ACHJ8C_001430"
     tRNA            26376..26451
                     /locus_tag="ACHJ8C_001430"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26409..26411,aa:Phe,seq:gaa)
     gene            26611..27873
                     /locus_tag="ACHJ8C_001431"
     CDS             26611..27873
                     /locus_tag="ACHJ8C_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5551315.1"
                     /translation="MALTDSKIRAAKPLAKSYKLTDSQGLYLTVSTSGSKLWYFRYSF
                     GGKESRLAFGPYPQITLAEAREKRDAARKLLAPRICPSQLLKPEKDTVDASRTFQFIA
                     TAWHTSCLKLWSEGHADKILTCLKRYVFPDIGAMDIAKIETRHLAQLVKAIDDKGVHD
                     VAGRVKQHLTKIMCHAVQQGIIKYNPAYDLDGVVTPVVTQHHPALPLKRLPELLEKTD
                     GYKGRELTRLALELNLHVFLRSSELRLARWDEFNLKAHIWTVPAKREAVKGVRFAERG
                     AKMKDEHLVPLSAQAVALLKQIKTITGESVFVFPGAHSLEKPMSENTINKALRVIGYD
                     TKTEVCGHGFRTMACSALNESGLWSKDAIERQMSHKERNGVRAAYVHKAEHLEARMEM
                     MQWWSDYLDMSREGYVAPYIYARQHKVA"
     gene            complement(28145..29026)
                     /locus_tag="ACHJ8C_001432"
     CDS             complement(28145..29026)
                     /locus_tag="ACHJ8C_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001228212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO5551316.1"
                     /translation="MRKLAQQMTTLAQQVGGSHKTVHDRMAIAKRFCHHLLALNIQSK
                     QVAHIRTRHIEGYIQHRLTQGISKRTLQNEMAALRAVLQQAGRDKLASDARLSNRTLG
                     LAGASRNGTKQAIPERAYQSALQKAQATDPGFAAALTLARLMGLRSQEAVQCAQSLKT
                     WRQALANGEQQLRVVFGTKGGRPRMTTVQDSVAVKQAVDRAISIAAERNGRLIDATDL
                     KTAMNYWHNQARRCGLTGEHSPHSLRYAWAQDAVRYWLAQGLSKKEALAQVSTDLGHG
                     DGRGRYIRQVYGRGMEE"
     gene            complement(29669..29884)
                     /locus_tag="ACHJ8C_001433"
     CDS             complement(29669..29884)
                     /locus_tag="ACHJ8C_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5551317.1"
                     /translation="MTQPDWHPADIMAALRKRGTSLAAVSRRAGLASSTLANAICRHW
                     PKGERLIAEELGVAPEQIWPSRYRKPE"
     gene            complement(29881..30351)
                     /locus_tag="ACHJ8C_001434"
     CDS             complement(29881..30351)
                     /locus_tag="ACHJ8C_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5551318.1"
                     /translation="MATILTPDCDLLDLLSVPFNATTDFTVLADYCARFAETLIETDD
                     PALRLALCGRLHACLALLQPTLHDPIPPHLVESLTVDVPPSIPQHFVSEPDSLCEYCL
                     TLLRLLLERPAEPKTEQTLTGLLCELVWYFTEGLKAPRWLRTADGVKFIGEVTV"
     gene            complement(31022..33217)
                     /locus_tag="ACHJ8C_001435"
     CDS             complement(31022..33217)
                     /locus_tag="ACHJ8C_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5551319.1"
                     /translation="MSILSIHGVRSYQGERPVEFDLSKYVTLIYGQNGSGKSTVSGYF
                     YKHGRPDYSQCNLQPPLNMNYLVFNQEYVDDIFSQPSQPGVFTLNSENAEIKTEIDAL
                     QAESKEMLDRRDALDVQKCDVEGMEESVKSDAAKQIYSSTAEIRKTDLWELMVGAKQT
                     NPLYQTILGHLKVEETSTQELTEELNRLEASKGNPYPLLEALPTSPLNESDIALLMQP
                     LIPAGDSRLAAAINQLGNIDWVRSGQKWLIDDICPFCQTPTDARQLQQEITALFDTSW
                     EIAMDQLRELQGRYQLWHDKPEHMRQLIKACPLVDLEHPVYLCLLELEQAYQRNKMHI
                     DEKLTSPSASISLEDLSALAGNVSAQIASINVAISEHNRKAENYQTEREGLKQKLLGH
                     IRKLATDTIINHDEQLAKLAEKSEKITVSQKEISAQLSALTATIRDKSSQIVNTQETI
                     DRINHGLNILGITGFHIASYDDRDDYRLVRSGEKSDVPVFSSLSEGEKTLIAFLYFLE
                     TCTGRKSRDDDDQRKRLIVIDDPISSLSQNYVFEIASLIQHQVIRAKIAEKVIILTHS
                     LFFFQELLLSAERKKRAAGSCPPEWTLYRISKSLHSSASLVSEKELLNDYQALWYVLR
                     HAQKDDIASVVIPNTMRQILEYYFGFSGKSETLHRALETLASGPEGEPGFRIFARYLN
                     RHSHQDARNISLHEGASVERYLAWFKKVFEAAKDEEHYTSMMEKTTQAA"
     gene            33417..34034
                     /locus_tag="ACHJ8C_001436"
     CDS             33417..34034
                     /locus_tag="ACHJ8C_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HFO5551320.1"
                     /translation="MSVAALARQRVHRMKRGIPFSISGFYSLGSRASVQQAMSRLVKE
                     GLLVRVERGFYSRPKPLKNLPSVNVVASANEVAAAWAKERGYKITLQGLEAAYRLGLQ
                     TQAPVKRIYWSNGPTREFRVGNELVRIQHKSASRLRWLNRPEGELMRGLLSLDQQHTP
                     SETLKNAFHRLSIPSAKARQIAGKLMNEPALHAWRSQLIALQKAL"
     gene            34034..35095
                     /locus_tag="ACHJ8C_001437"
     CDS             34034..35095
                     /locus_tag="ACHJ8C_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFO5551321.1"
                     /translation="MNIFDYYRNASQRRELQEILSYAAQQHPSGLGASFLEKDLWVTE
                     ILRLLFNEDLLGDLSVAFKGGTALSKCWNVIERFSEDIDLSVHWADLAGHSEEEEQQA
                     WEQSTLNNSQNRRFREQQQERLTTWTSALAERLNQRFQAYEIPGLEAKMEPDSSGEKI
                     DINFPRVKEDNMAYQRDHILLEFGGRNRGKPTDLMPVVSYLSGIAGMNALLLPAATVN
                     AYDTGYIFWEKLTALHQFCTQTKAPNPARLARHWYDVDCLLRNNFASPYETQEAMRNV
                     VEMKQRRWSVPGVDFTQVITGNLQLLPDDAERFAAIAGDHQTAVEGGMFFREPDGFAD
                     IADRLRHVQDEINRFMYGS"
     gene            35447..36406
                     /locus_tag="ACHJ8C_001438"
     CDS             35447..36406
                     /locus_tag="ACHJ8C_001438"
                     /inference="COORDINATES: protein motif:HMM:NF024606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB N-terminal domain-containing protein"
                     /protein_id="HFO5551322.1"
                     /translation="MHSQESDDDELVTFSFVNGQAIEYSTSRQPSRESAARSNTTPAR
                     WIKPGESIIIQNIVIKNGNFYFGGQLKTHSSGEYRYLYNDGSDASLVNDSLSIEPSSR
                     HYYDESLGYWPTFATLSPRCRGAYLDWLASDRSDASCPVGYVFIYFYGLERRVLADGT
                     QEAISDDEFKALFEEISRLRIVFQASGSFRHYATQLLEMMLILRPKLLSIHTENEYFS
                     SRSALLFRLNLATVVDKGQPVCAELALAWIHYFPDYTLRTPARRCHAEFSALFKQRYT
                     RKYGDGIVVKPNKTRLYLSYTPASGTLRELQVKNRWFFPIPAF"
     gene            36379..36822
                     /locus_tag="ACHJ8C_001439"
     CDS             36379..36822
                     /locus_tag="ACHJ8C_001439"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5551323.1"
                     /translation="MVLPDPSVLKAPVQKLIPVAESCVNALDAYSRYLGKKDVSASDV
                     AAIMLLPDEILTEDAGRLFAEFKHWADEKIREHSGLATVADFWARLGMLVPDKINKKE
                     AELMQNFARRAGYGIAPDMRYELNPKTWTVSVNRLFDTQSGILPG"
     gene            complement(36779..37943)
                     /locus_tag="ACHJ8C_001440"
                     /pseudo
     CDS             complement(36779..37943)
                     /locus_tag="ACHJ8C_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1397 family transposase"
BASE COUNT         9097 a         9906 c         9510 g         9430 t
ORIGIN      
        1 ataaatcgac ccgccagcgg aataatctta attattcatc ttcaagatag gtataaccgt
       61 acaaacctgc ctcgaactct tcaaggaact gttgttgcag ttcagcatca agatcggttt
      121 tcttcacttg atcacggaac tgggttaaca acgttttcgg atcgagctgc acatattgca
      181 gcatgtccgc cacggtatcg ccttcgtcag acagttccac ttctacacta ccgtcaggga
      241 agacgaacac gtcaaccgct tcggtatcac cgaacaggtt gtgcatgttg ccgaggatct
      301 cctgatatgc gccgaccata aagaaaccga gcatcggcgg attctccgga tcgtactccg
      361 gcataggcat tgtcgtggca ataccatcac catcaatata gtggtcgata gcaccgtctg
      421 agtcacaggt aatatccagc agcacagcgc ggcgttccgg cacttgatcc agcccttcca
      481 gcggcagaac cgggaacaac tggtcgatcc cccacgcatc cggcatcgac tggaacagcg
      541 agaagttgac gtacattttg tccgccatac gttcctgcag ctcgtcgata atcggacgat
      601 gagcacggtt ttgcggatcc agctgctttt gcacttcatg gcacatgctc aaataaagct
      661 gctcagccca tgcgcgttct tgcaggctaa aggtgccgga agagtagccg atatgaatat
      721 cgtgcagatc catctggctg tcgtgtaacc attcacgcag agaacggcga gttcccggtt
      781 cgtgcatttc ctgccaggtt tcccacatgc tttgcagggc gcgcggtgcg tcttctgcag
      841 gcgcggtcgg caccgtgtat tcgttacgtt ccacgccgat gatattcgac accagcacgg
      901 tgtgatgcgc agtcaccgca cgacccgatt cggtgattac cgtcggatgt ggcagaccat
      961 tttcttcaca cgcatcacca atcgcccaga tgatgttgtt ggcgtattca ttgaggccgt
     1021 agtttacgga gcagtcagac tgcgaacggg tgccttcata gtccacgccc agaccgccgc
     1081 cgacgtcgaa gcactgaata ttgacgccca gcttgtgcag ttccacatag aaacgcgcgg
     1141 attcacgaac gcctgtcgcg atatcgcgaa tattcgccat ctgcgaaccg aggtggaagt
     1201 gcagcagttg cagactgtcg agacgcccgg cttcacgcag cgtttcaacc agttgcagta
     1261 cctgagtcgc cgccaggcca aacttcgatt tttccccgcc ggaggactgc catttacccg
     1321 agccttgcga cgccagacgt gcacgcacgc ccagacgagg aacaacattc agacgttctg
     1381 cttcatccag cacaatggca atttctgaca tcttctcaat gaccagatag accttgtggc
     1441 ccatcttctc gccaattaat gccaggcgga tatattcgcg gtccttataa ccgttgcaga
     1501 cgatgacgct acgggtcatg ccagcatgtg ccagcaccgc catcaactca gctttggaac
     1561 cggcttccag gcccagcggt tcgcccgaat gaatcaggga ctcaatcacc cggcggtgct
     1621 ggttaacttt gatcggataa acgaggaagt aatcgccgtt atagccgtag gattcccgcg
     1681 ctcgtttgaa cgcggcgtta atggaacgca aacggtgctg caggatctgt gggaaacaga
     1741 acagtgcagg cagacgctga ccctgtgctt cgcgagtttt cactaactgc gcgagatcga
     1801 cgcgagcttc cgggacgtcc gggtccgggc acacgctgat gtggcccagc tcgttaacgt
     1861 catagtagtt attgccccac caggcaatat tgtaagtacg cagcatcttg ctggcttcct
     1921 gggagctcat tgcaacctcc tgcatggagc gtagtacgcc gtgttcgccc gctgacgaag
     1981 gcaaacccat agacatgtcg tcagacatag cgaacctcaa attattttat taagtgtaaa
     2041 acagttaacg actatcgcag cctggaactg cgataacaac ccataagcac atggattttc
     2101 cagcagtgaa tgctgacgct ccaactgcgc aaccggtttc tttttcataa cattattaag
     2161 cacataaccg aacgtaagtg tgacagttcg gcgaaaccac gagaaaactc ttgtttttac
     2221 aagagcgccc ttgttcagtc ctcagtagct gtaaccagct cttgaatcct gagaagcgcc
     2281 gagatgggta taacatcggc aggtatgcaa agcagagatg cagagtgcgg ggaacgaatc
     2341 ttcaccagaa cggtgagaca ggttaagcag cagacaacgg ttcattattt cgtatcacct
     2401 ccacggccgc ctgttaagac gaacccacaa gccaaaactc tgttttcaac ccggctggaa
     2461 gtggcaacac gaaagaaacg tcgtgtgctt tttatttaag ccgcgcgccg cgttttatac
     2521 cccacaatgg cagaaaattg caaaagataa atacgcagaa tgccagcatt gtcaggaaaa
     2581 atttccagcc acgtttttaa cagaatgaga cacgattcaa aaaaaagtgg aaattgggtg
     2641 aagaactgac ctaaaatagc catccagatg ttaatccatc cataccgatt aacactcaga
     2701 ctgccagtgt ttttaacctg cagagtcgtg gtaggatccg ctaccacaga aaatccacac
     2761 aacagtttga gctaaccaaa ttctctttag gtgatattaa atatggcaaa acaccttttt
     2821 acgtccgagt ccgtctctga agggcatcct gacaaaattg ctgaccaaat ctctgatgcc
     2881 gttttagacg cgatcctcga acaggatccg aaagcacgcg ttgcttgcga aacctacgta
     2941 aaaaccggca tggttttagt tggcggcgaa atcaccacca gcgcctgggt agacatcgaa
     3001 gagatcaccc gtaacaccgt tcgcgaaatt ggctatgtac attccgacat gggctttgac
     3061 gctaactcct gtgcggttct gagcgccatc ggtaaacagt ctcctgacat caaccagggc
     3121 gttgaccgtg ccgatccgct ggaacagggc gcaggtgacc agggtctgat gtttggctac
     3181 gcaactaatg aaaccgacgt gctgatgcca gcacctatca cttatgcaca ccgtctggta
     3241 cagcgtcagg ctgaagtgcg taaaaacggc actctgccgt ggctgcgccc ggacgcgaaa
     3301 agccaggtga ctttccagta tgacgacggc aaaatcgttg gcattgatgc tgtcgtgctt
     3361 tccactcagc actctgaaga gatcgaccag aaatcgctgc aagaagcggt aatggaagag
     3421 atcatcaagc cgattctgcc cgctgaatgg ctgacttctg ccaccaaatt cttcatcaac
     3481 ccgaccggtc gtttcgttat cggtggcccg atgggtgact gcggtctgac tggtcgtaaa
     3541 atcatcgttg atacctacgg cggcatggcg cgtcacggtg gcggtgcatt ctctggtaaa
     3601 gatccatcaa aagtggaccg ttccgcagcc tacgcagcac gttatgtcgc gaaaaacatt
     3661 gttgctgctg gcttggccga tcgttgtgaa attcaggttt cctacgcaat cggcgtggct
     3721 gaaccgactt ccatcatggt agaaactttc ggtactgaga aagtgccttc tgaacaactg
     3781 actctgctgg tacgtgaatt cttcgacctg cgcccatacg gtctgattca gatgctggat
     3841 ctgctgcacc cgatctacaa agaaaccgca gcatacggtc actttggtcg tgaacatttc
     3901 ccgtgggaaa aaaccgacaa agcgcagctg ctgcgtgatg ctgccggtct aaagtaatct
     3961 ttcttcacct gctttcaaag gccagcctcg cgctggcctt tttcttttac ctcaccgcgg
     4021 tggacaggca ttcacgccgc atccggcaaa aaaatcgccc gcacaataac atcattcttc
     4081 ctcaacacgt tttaccgcaa attatcatca ctactgaaac cgattacacc aaccacgcca
     4141 gccaaatatt tgtaatattt tcatattatt attcagcctt cacagttgtt acatttcttt
     4201 tcagtaaagt cttaattgca gataacagcc tttaatctat gatgatataa ctcaattatt
     4261 ttcatacact taaatcataa ctaagataaa tgttagtgta agcgattaca ctgatgtgat
     4321 ttgcttcaca tctttttacg tcgtactcac ctatcttaat tcacaataaa aaataaccat
     4381 attggagggc atcatgcctg acgctaaaaa acaggggcgg tcaaacaagg caatgacgtt
     4441 tttcgtctgc ttccttgccg ctctggcggg attactcttt ggcctggata tcggtgtaat
     4501 tgctggcgca ctgccgttta ttgcagatga attccagatt acttcgcaca cgcaagaatg
     4561 ggtcgtaagc tccatgatgt tcggtgcggc agtcggtgcg gtgggcagcg gctggctctc
     4621 ctttaaactc gggcgcaaaa agagcctgat gatcggcgca atcctgtttg ttgccggttc
     4681 actgttctct gcggctgcgc caaacgttga agtactgatt ctttcccgcg ttctgctggg
     4741 gctggcggtg ggtgtggcct cttataccgc accactgtac ctgtcagaaa ttgcgccgga
     4801 aaaaattcgc ggcagtatga tctcgatgta tcagttgatg atcactatcg ggatcctcgg
     4861 cgcttatctt tctgataccg ccttcagcta caccggtgca tggcgctgga tgctgggtgt
     4921 gattatcatc ccggcaattt tgctgctgat tggtgtcttc ttcctgccag acagcccacg
     4981 ttggtttgcc gccaaacgcc gttttgttga tgccgaacgc gtgctgctac gcctgcgtga
     5041 caccagcgcg gaagcgaaac gcgaactgga tgaaatccgt gaaagtttgc aggttaaaca
     5101 gagtggctgg gcgctgttta aagagaacag caacttccgc cgcgcggtgt tccttggcgt
     5161 actattgcag ataatgcagc aattcaccgg gatgaacgtc atcatgtatt acgcgccgaa
     5221 aatcttcgaa ctggcgggtt ataccaacac caccgagcaa atgtggggga cagtgattgt
     5281 cggcctgacc aacgtacttg ccacctttat cgcaatcggc cttgttgacc gctggggacg
     5341 taaaccaacg ctaacgctgg gcttcctggt gatggctgct ggtatgggcg tactcggtac
     5401 aatgatgcat atcggtatcc actctccgtc ggcgcagtat ttcgccatcg ccatgctgct
     5461 gatgtttatt gtcggttttg ccatgagtgc tggtccgctg atttgggtac tgtgctccga
     5521 aattcagccg ctgaaaggcc gcgattttgg catcacctgc tccactgcca ccaactggat
     5581 tgccaacatg atcgttggcg caacgttcct gaccatgctc aacacgctgg gtaacgccaa
     5641 caccttctgg gtgtatgctg ctctgaacgt actgtttatc ctgctgacat tgtggctggt
     5701 accggaaacc aaacatgttt cgctggaaca tattgaacgt aatctgatga aaggtcgtaa
     5761 actgcgcgaa atcggcgctc acgattaatc tcctcaggct tcctcccgtc gtggaggaag
     5821 ccacctcttg cagtcatctt ttcttcgctc tatcctctgc cgctatgaaa acatcccgtc
     5881 tccctatcgc cctccaacag gccgttatgc gtcgcctgcg ggaaaaactc gcccaggcga
     5941 acctgaagct agggcgtaac tacccggagc caaaactctc ttacacccag cgcggaacct
     6001 ctgccggaac agcctggctg gaaagctatg aaattcgcct caatcccgtt ttgctgttgg
     6061 aaaacagtga agcctttatt gaagaagtgg tgccgcacga actggcacat ttgctggtat
     6121 ggaaacattt cggccgcgta gcgccacatg gcaaagagtg gaagtggatg atggaaagcg
     6181 tgctgggcgt tcccgcccgc cgtacgcatc agttcgaact gcaatccgtg cgccgcaaca
     6241 ccttccccta ccgctgcaag tgccaggagc atcagcttac cgtacgccgt cataatcgcg
     6301 tagttcgtgg cgaagccgtc tatcgctgta ttcactgcgg cgaacagctg gttgcgaaat
     6361 aaccacctga actatcagga actttcctga tctggctgat tgcataccaa aacaactttc
     6421 gctacgttgc tggctcgttt taacacggag taagtgatgt accgttattt gtctattgct
     6481 gcggtggtac tgagcgcagc attttccggc ccggcattgg ccgaaggtat caatagtttt
     6541 tctcaagcga aagccgcggc ggtaaaagtc cacgctgacg cgcccggtac gttttattgc
     6601 ggatgtaaaa ttaactggca gggcaaaaaa ggcgttgttg atctgcaatc gtgcggctat
     6661 caggtgcgaa aaaatgaaaa ccgtgccagc cgcgtagagt gggaacacgt cgttcctgcc
     6721 tggcaattcg gtcaccagcg ccagtgctgg caggacggtg gacgtaaaaa ttgcgccaaa
     6781 gatccggtct atcgcaagat ggaaagcgat atgcataacc tgcagccgtc agtcggtgag
     6841 gtgaatggcg atcgcggcaa ctttatgtac agccagtgga atggcggtga aggccagtac
     6901 ggtcaatgcg ccatgaaggt cgatttcaaa gaaaaagttg ccgaaccacc agcgcgtgca
     6961 cgcggtgcca ttgcgcgcac ctacttctat atgcgtgacc aatacaacct gacactctct
     7021 cgtcagcaaa cgcagctgtt caacgcatgg gacaagatgt atccagttac cgactgggag
     7081 tgcgagcgtg atgaacgcat cgcgaaggtg cagggcaatc ataacccgta tgtgcaacgc
     7141 gcttgccagg cgcgaaagag ctaacctaca ctagcgggat tctttttgtt aacccctacc
     7201 ccacgcgtac aaccgcgtgg ggagacgacg cggattttta actatgcgta tcccccgcat
     7261 ttatcatcct gaaccactga ccagccattc tcacatcgcg ctttgcgaag atgccgccaa
     7321 ccatatcggg cgcgtactgc gtatggggcc gggacaggcg ctgcaattgt ttgacggcag
     7381 caaccaggtc tttgacgccg aaattaccag cgccagcaaa aaaagcgtgg aagtgaaggt
     7441 gctggaaggc cagatcgacg atcgcgaatc tccgctgcat attcacctcg gtcaggtgat
     7501 gtcgcgtggt gaaaaaatgg aatttactat ccagaaatcg atcgaactcg gtgtaagcct
     7561 cattacgcca cttttttctg agcgctgcgg cgttaaactg gatagtgaac gtctgaacaa
     7621 gaagctccag cagtggcaga agattgcaat tgctgcctgt gagcagtgtg gccgtaaccg
     7681 ggtgccggaa atccgtccag cgatggatct ggaagcctgg tgtgcagagc aggatgaagg
     7741 actgaaactg aatcttcacc cgcgcgccag taacagcatc aatacgttgc cgttaccggt
     7801 cgaacgcgtc cgcctgctga ttggcccgga aggcggttta tcggcagatg aaattgccat
     7861 gactgcccgc tatcaattta ctgatatcct gttgggacct cgcgttttgc gtactgagac
     7921 aactgcgctc accgccatta ccgcgctaca agtgcgtttt ggcgatttgg gctaacggag
     7981 aagaataatg atcaagctcg gcatcgtgat ggaccccatc gcaaacatca acatcaagaa
     8041 agattccagt ttcgctatgt tgctggaagc acagcgtcgt ggttacgaac ttcactatat
     8101 ggagatggcc gatctctatt tgatcaatgg tgaggctcgc gctcgtaccc gtactttgag
     8161 cgtcgaacag aactacgata aatggtacga gttcacgggt gagcaggatc tgccgctggc
     8221 cgatctcgat gtgatcctga tgcgtaaaga cccgccgttt gataccgagt ttatctacgc
     8281 gacctatatt ctggaacgtg ccgaagataa agggacgctg atcgttaaca agccgcagag
     8341 cctgcgcgat tgtaacgaaa aactgttcac tgcctggttc tctgacttaa caccagaaac
     8401 gctggtcaca cgcaataaag cgcagctaaa agctttctgg gaaaaacaca gcgacatcat
     8461 tcttaagccg ctggacggta tgggcggcgc gtcgattttc cgcgtgaaag aaggtgatcc
     8521 aaacctcggc gtgattgccg aaaccctgac tgagcatggc actcgctact gcatggcgca
     8581 aaattacctg ccagccatta aagatggcga caaacgcgtg ctggtagtgg atggcgagcc
     8641 ggtgccgtat tgcctggcgc gtattccgca ggggggcgaa acccgtggca atctggctgc
     8701 cggtggtcgc ggtgaacctc gtccgctgac ggaaagtgac tggaaaatcg cccgtcagat
     8761 tgggccgacg ctgaaagaaa aagggctgat ttttgttggt ctggatatca tcggcgaccg
     8821 tctgactgaa attaacgtca ccagcccaac ctgtattcgt gagattgaag cagagtttcc
     8881 ggtgtcgatc accggaatgt taatggatgc catcgaagca cgtttacagc agcagtaacc
     8941 caccttagcg agaaggatct cgttgagact ctgagtgaca gcgcccttct ttccacgcat
     9001 actgggcgct gttgcttttt tgaaccagga aacagaacct ctgacaatga atttacagca
     9061 tcactttctt attgccatgc ctgctctcca ggatccgatt ttccgtcgtt ccgtggtcta
     9121 catttgcgaa cataatacca atggtgcaat ggggatcatc gtcaacaagc cgctggaaaa
     9181 tctcaaaatt gaagggattc tggaaaagct gaagatcacg ccggagccgc gtgatgaatc
     9241 aatccgtctg gataaaccgg ttatgctcgg cggtccgctg gctgaagatc gcgggtttat
     9301 tttgcatact ccgccctcca attttgcttc cagcattcgc atttcagaca acacagtgat
     9361 gaccacttcc cgcgatgtgc tggaaacgct cggcaccgat aaacaaccgt ctgacgtatt
     9421 ggtggctctg ggttatgcct cctgggagaa aggtcaactg gaacaagaaa ttctcgataa
     9481 cgcgtggcta acggccccgg cagatctgaa tattctgttc aaaacgccga ttgcggaccg
     9541 ctggcgcgag gcggcaaaac tgattggtgt ggatattctc accatgcctg gtgtggcagg
     9601 acacgcctga tgagtggaac cttactcgcc ttcgacttcg gcaccaaaag cattggcgta
     9661 gcggtcggcc aacgcattac cggcaccgct cgccctttgc ctgcaattaa agcgcaggac
     9721 ggtacgccgg actggaacat tatcgagcgt ttactgaaag agtggcagcc ggacgaaatc
     9781 atcgtcggtt tgccgctgaa tatggacggc accgaacagc cgttaaccgc cagagcgcgt
     9841 aaatttgcca accgtattca tggtcgtttc ggtgttgaag taaagctcca tgacgagcgt
     9901 cttagcaccg tggaggcacg ttccggtctg tttgaacagg gcggctatcg ggcgctcaac
     9961 aaaggcaaag tcgactctgc ctctgcggtt attattctcg aaagctattt cgagcaggga
    10021 tattaaggcg atttactagc gcacagattt ctgacaatgt gctcgttgtt tcatgttgga
    10081 tgcggcgtaa acgccttatc caacctacag tgagtgcgaa ttcaaagtta cgcttccctt
    10141 gtaggcccga taagcttgcg catcgggcat atttacaatt cgtatcagac acagcaacgt
    10201 cacaagcgcc cttctttcac ccgctgctga taactctgct gaaacgttat catccccacc
    10261 tgctgcccgg tttgaataac atgcggtaac tggtgggttt taccttctcg aatcaaattc
    10321 cccaccgcgg gtgtgttaat cagtaattca aacagcgcca cgcgtccttc ctgtttatcc
    10381 acctccagtt tttgtgacaa cactgcccgt aaactacctg ccagttgatt acgcacggga
    10441 tctttttcct gcgcaggaaa tgaatccacc agtcgctcaa ctgcctgcgc cgcaccacgc
    10501 gtatgtaatg ttgccagcac cagatgcccg gtttctgccg ccgtcagtgc cagacggatt
    10561 gtctcgctgt cacgcagctc tccgagcaaa atcacatcag gatcttcccg caatgcggcc
    10621 cgcaatcccg atgcgaacgt catacagtgc aaaccaattt cccgctgctg gatcaaacat
    10681 cgctggctgg catagagata ttccacagga tcttccagcg tcagaatatg cgcatcagca
    10741 tgttgattaa gatagccaac catcgccgcc agcgtggtag atttgccgct ccccgtcgcc
    10801 cccgtcacca gaatcaggcc attctcgctc ttgagtaatt ccggcaatac cggtggtgcg
    10861 ccaagctgtt cgagctgcgg gcagtgcgaa ggtaacagcc gtaacgccag agaaatacct
    10921 tgccgttgtg cgaacgcact gccgcgcaat cgctggtttt ccgccagcga cacagcaaaa
    10981 tccagctgac cattctccag caatattgcc cgctgatcgt catccagcca ctcccgcagt
    11041 agctcttcga cgtccggtgc gtcaaacggc gcagcttcca ttctcccgcg aatacgccat
    11101 cgtgcgggcc aggcgctgca caggtgtaga tccgagacgt tatgctttac actaagggcc
    11161 acaatttctt ccatattcat actaagatcc tcggaaaatg aacgatattg cgcataacct
    11221 ggcacaggtc cgggacaaaa tctcagcggc tgcaacgcgt tgcggccgtt ctccagaaga
    11281 aattacgctg cttgcagtca gtaaaacaaa acctgcgagc gccatcgcag aagccattga
    11341 tgccgggcag cgtcaatttg gtgaaaacta cgttcaggaa ggggtagata aaattcgcca
    11401 ctttcaggaa ctgggcgtaa caggattaga atggcatttt attggcccgt tgcagtctaa
    11461 taaaagccgc ctggtggcag agcatttcga ctggtgtcat accatcgacc gtttgcgcat
    11521 cgctacccgc ctgaacgacc agcgcccggc agaacttccc cctcttaacg tactgattca
    11581 aattaacatt agtgatgaaa acagtaagtc cgggattcaa ctggctgaac tggacgagct
    11641 ggcagctgcg gtcgctgaac taccgcgttt acgtctgcgc gggctgatgg caatccctgc
    11701 gcctgagtca gaatatgtaa ggcagtttga agttgcacgc caaatggctg tagcatttgc
    11761 cggactgaaa acgcgctacc cgcatatcga cacgctctct ctgggaatgt cggacgatat
    11821 ggaagccgcc attgcggcag gtagcacgat ggttcgtatc ggcactgcaa tttttggtgc
    11881 gcgtgattac tctaaaaaat aaggaattaa aggaacgcca tgaatacgtt gactttcctg
    11941 ctttcaacgg tcattgagct gtataccatg gtgctgttat tacgcatctg gatgcagtgg
    12001 gctcattgtg atttttacaa ccccttctca cagtttgtag tgaaggtaac gcagccgatt
    12061 atcgggccac tgcgccgcgt tattccggca atgggcccca ttgatagcgc ctcgctgctg
    12121 gttgcctata ttctcagttt cattaaagcc atcgtgctgt ttaaagtggt gaccttcctg
    12181 ccaatcattt ggattgccgg tttattgatt ctgctgaaaa ccatcggcct gctgattttc
    12241 tgggtcctgc tggtgatggc gattatgagc tgggtaagcc agggtcgtag cccgattgaa
    12301 tacgtgctga ttcagctggc cgatccgctg ctgcgcccga ttcgccgctt gctaccggca
    12361 atgggtggga ttgatttctc gccgatgatc ctcgttctgc tgctgtatgt catcaatatg
    12421 ggtgtcgcag aagtcttgca ggcgaccgga aatatgctgc tgccggggct gtggatggcg
    12481 ttatgagtgc cgtaacagtt aatgatgacg gtctggtttt acggctctat attcagccga
    12541 aagccagccg tgattctatt gtcggtttac atggcgacga ggtaaaagtc gccattaccg
    12601 cgccgccggt tgacggccag gccaacagcc atctggtgaa gtttctcggt aagcaattcc
    12661 gggttgccaa aagccaggtg gtgattgaaa aaggcgaact tggccgccac aaacaaatta
    12721 aaatcattaa tccgcaacaa atcccgccag aaatcgcggc gttaattaat taggtatcct
    12781 atgcaaaaag ttgtcctcgc aaccggcaat gccggtaaag tgcgtgagct ggcgtcgctg
    12841 cttagcgact tcggtcttga tatcgttgcc caaacagacc tcggcgtaga ttccgctgaa
    12901 gaaaccggcc tgacctttat cgaaaacgcg attctgaaag cgcgccatgc ggcaaaagtg
    12961 accggtttac cggcaattgc cgacgactcc ggtctggcgg tagatgcgct gggtggcgcg
    13021 ccgggtattt actccgcgcg ttattccggt gaagacgcga ccgatcaaaa gaatctgcaa
    13081 aaactgctgg aaacaatgaa agacgtaccg gacgaccaac gtcaggcgcg tttccactgc
    13141 gtgctggtat atctgcgtca cgcggaagat cccactccgc tggtatgcca cggtagctgg
    13201 ccgggcgtga ttactcgtga accagcgggc actggtggct ttggttatga tccaatcttc
    13261 ttcgtacctt ccgaagggaa aaccgctgcc gaactgaccc gcgaagaaaa gagcgccatt
    13321 tcccaccgtg gtcaggcgtt gaaactgctg ctggacgctt tacgtaatgg ttaaattacc
    13381 gccgctgagt ctctacattc acatcccgtg gtgcgtgcag aaatgcccgt actgcgattt
    13441 caactctcac gcgttgaaag gagaagtgcc gcacgacgat tatgttcagc atctgcttaa
    13501 cgatctggac aacgatgtgg cttacgctca gggccgtgaa gtaaagacaa tctttattgg
    13561 cggtggtacg ccgagcctgc tttccggccc ggcgatgcaa acgctgctgg acggcgtacg
    13621 tgcgcgtttg ccgctggcag cggatgcaga aattacgatg gaagcgaacc ctggtacggt
    13681 tgaagccgat cgctttgttg attatcagcg tgctggtgtg aaccgcatct ccattggcgt
    13741 acagagtttt agcgaagaaa agctgaaacg acttgggcgc attcatggcc cgcaagaagc
    13801 gaaacgagcg gcaaatctgg cgagcggttt agggttacgt agctttaacc tcgatttgat
    13861 gcatgggcta ccggatcaat cactggaaga ggcgcttggc gatctacgcc aggctattga
    13921 actgaatccg ccgcatcttt cctggtatca actgaccatc gaacccaata cgctgtttgg
    13981 ctcgcgccct cctgtactgc cggacgatga cgcgctgtgg gacattttcg aacaggggca
    14041 tcagttatta accgcagcgg gttatcagca atatgaaact tccgcttacg ccaaacccgg
    14101 ttatcagtgc cagcacaatc tcaactactg gcgctttggt gactacatcg gtattggctg
    14161 cggcgcgcat ggcaaagtga ccttcccgga tgggcgcatt ctgcgtacca ccaaaacgcg
    14221 tcatccacgt ggttttatgc aggggcggta tctggaaagc cagcgtgatg tcgaagccgc
    14281 agataaacca tttgagttct ttatgaatcg cttccgtttg ctggaacctg cgccgcgcgt
    14341 ggagtttagc cagtatactg gcctttcaga agaggttatt cgccctcagt tagacgaggc
    14401 tattgctcag ggttatctca cagaatgtgc ggattactgg cagataacgg aacatgggaa
    14461 gttgttttta aattcgctgc tggagctttt tctggctgag taaatttgta ttgccggatg
    14521 cggcgtgtac gcaacatccg gctacaactc ttgcgtcatc gcaaaaatta cttaatcacc
    14581 ccattgcgcc gcaattgttc ggtatctttt ttccagcgtt cattaacctg gttgatatcg
    14641 tcaattttca tcaaaatcgt gactttttta ctgtttattt ctgaaacttt catcgttaac
    14701 ggatattgcc ctttttcgag cgttatccag tcatcctgca atttacggct aattttttgt
    14761 gattgcagaa aagtctgtcg ccgttgttcg ttgatatcaa aatctcgttt ctgctgaatt
    14821 ggcacttcat aacactcttc actctgttta tgacatgccg caatcgctgg ttgataaacc
    14881 tctttgtcat atttaaccgc gaacgcctca ctatcgttct gtttattaaa atcatcacgc
    14941 tgttggtgaa tttttttaaa ggcgtcttcg cgggtcattt gcttaccgtt tgcatctttt
    15001 acccaatatg catccgctgc tttctgcgcc gcaaccattt gcttatcaag cgcttttaca
    15061 tccttttcca gcgcaggaat cgagttattc tggttattca atgcagactc aagctgacta
    15121 aagcgataat tgaaatcttc gctattcacg atcagatatt tattattcgt tttgatatcg
    15181 tcaaccaccc gcccacgatc gctggctgcc gcctgaaaag agtaaaagtt caccgaccag
    15241 ccagacgcgg gcgttccttt actggttaac atcgccgaga atttcaccgg tttatctttc
    15301 gtccaggtct gttcgagcag ctcgtaatct gccaactgac cgacccaggt atagagatcg
    15361 tcactggaag agacatcgcc ttccgctgac caggtggcct gattaccctt agcatcaaga
    15421 ttttttaagg tgatgacatc aagcttgaga atgccgtgat actgcttttt gaattgatct
    15481 tttaagatat tttcagttgg cggttcacca tctgcccgga cattaccagt catcaacata
    15541 aacagcgccg taccaacaat ccattgcttt ttcacccgaa aaccctcgcg cggaataatt
    15601 tattccggtg attatcatta gggtaataaa aattggcaat ggacgtgagt ctgaagtgaa
    15661 taagccccgg cacgataccg gggcgaagtg attagtactg attgaagatc tgctggatct
    15721 gctgcggatc tttagtttgc gtcagagcca gttgcagcag aacgcgcgct ttttgcgggt
    15781 tcaacgtgcc agaggcgacg aagccgtatt tcgcatcatc cacttcggca tcctgagtgg
    15841 tagcgcccgt cggtacgcgg gaagaacgca ctactgcagt gccgtttttc gcagcggttg
    15901 caagggtgtc aaatacggtt ttatacaggt tgccgttacc cacgccagcg ctaacgatgc
    15961 catcatagcc cgcatctacc agtgctttag ccggaagatc ggatgcgtta gcgtagttat
    16021 aaacaatgcc gactttcggc agttcattca gcttagagac atcgaacggc gtgtcgctgg
    16081 tgtgcttacg taccggggta cgttggtagt caatcttacc gttgtgaatg taacccagag
    16141 gaccgtagtt aacagacttg aaggtcgcta cgtcggtggt gttggttttg gtgacatcac
    16201 ggccatcaag cacggtgtcg ttcatcacta ccagtacgcc acgattagcg gaggctttat
    16261 cagctgcggt cactaccgcg ttatacaggt tgaatggacc gtctgcgctc atagacgtgg
    16321 acgggcgcat tgcaccgacc atcaccaccg gtttgtcgca tttcaccgtc aggtcgagga
    16381 agtaagcggt ttcttccatc gtgtcggtac cgtgggtaat gacgaagccg tcagttttat
    16441 cgcagtcggt gttaattttt ttcgccagtg tcagccagac atcatcgttc atgtcctggg
    16501 agccaatatt cactacctgc tcgcctttaa cgttcgcaat gtccttcagt tgcggcaccg
    16561 cattaaccag attttctacg ccaactttac ccgctgtgta gttagatttg gttgcggagt
    16621 caccaccacc ggcaatggtc ccgccggttg ctaaaatggt gatattgggt aatgccaatg
    16681 ctgcaccact aaaacccata accagtgcgg caagtgccgt ctttttgaaa aactccattt
    16741 cattcctcca gttacgtgaa cgctacgcat tatcccttag ctttgtatgg gaaacttgac
    16801 gttaaacaat ttacaacatg aatatatttt ggagatctac aaagttagag gcaggtaaca
    16861 aaacgaagaa ttaaacggca taaaaaagta ttatgccgtc ttaaaataga ggattatttt
    16921 aaattcccga ccagggcttt gcggctatct tccagagtca caacgcggct acaaacatct
    16981 ttgccaaact gctggaaatc tttttcctgc tttttccact cggtttggat tgaggattgc
    17041 aggccgccca ggcttcccag cacgttctgt aatgggttac cgccgctttt cagcaccgct
    17101 ttcgcgccca tttcattaat gctgtcctgt aaaattccgc ccattgcctg attcactaat
    17161 tgctggcctt cggcacgaac ctgatcaatg gctttatagt gaaacgtcag gccatcgctg
    17221 cgcgtttcga taatgcggtt catctgctct ttcagctgcg catcaagttt ggtcagacgg
    17281 ctgcgcattt tgctgctttc gcccatctcc tgaacgataa ttttatccag cgcaatacgg
    17341 gctttctcga cgcggctttt cgcgccttca tcaatccacg gcagggtgct acgtagttca
    17401 gcctgataat ccttcgcctg ctcgcgctgg gcggcattca gggaatattg cttaccgtta
    17461 tacatcacgt tgccgtctgg cgtgatcacc agattgccgt tttcgccctt cacctgcacg
    17521 gtttgcgggc tgacaatcac atcgtcacgc ggcgtgacgc tgcactggta gtcggcgtga
    17581 gcggtcattg ccgtcactga aagtgctgcc gccagcagca ttttgcgcat catagtcttc
    17641 cctcaagaac aaatcaggcc agcatttgct ggccccagat tgctgacaaa gtacacgttt
    17701 tccatgccgg atgcggcgta aacgccttat ccagcctact cgatcgtgca tctttgtagg
    17761 cctgataagc gtagcacatc aggcagtttt gcatttgtca tcgcccgtat gctttcttag
    17821 tcccaccaaa cgtcgaaaag ttcgctggtg cgtacctctt ccagtttgcg ttcttccagc
    17881 cacttacgca caatcgcctg atgctcttcg gtgcatttgc cgatttcctg catgcagatc
    17941 agaccttccc aggccagata accgctgccg tcaaaggcca gtttgttcgg ttcgataacc
    18001 tcgttaataa agtcatcaac ggttttatca atctgttctt ccgatgtacc ttccgggaat
    18061 cgccatgcca ccgaaaatcc taattcctgg aattcgtcga tgtgcatttt tttacgcaga
    18121 cgacggctac ggttctttgc cattatttca ccctctcgaa cattaagtcc catactccgt
    18181 gaccaagacg atgaccacgt tgttcaaatt tcgtcaccgg acgtgatgcc ggacgcggta
    18241 cgtaatcatt gctctctgac aggtttttat aaccgtcaat agaagacatc acttcaagca
    18301 tatgttccgc ataaggttcc cagtcggtcg ccatatggaa tatgcccccc agctgcagtt
    18361 tgctttttac cagctctgca aacggcacct gaacgatacg gcgtttatta tggcgcgctt
    18421 tgtgccacgg gtcagggaaa aagagctgca ccatgcgcaa tgaattgtca ggaatcattt
    18481 tatgcagcac ttcaaccgca tcgtgacaca tcacgcgcag gttgcttaag ccctcttcat
    18541 gcgcagaaga caggcacgca ccaacacccg gtgaatgcac ttcaatgccg aggaagtcct
    18601 gctcggggcg atctttagcc attgccacca gcgacgcgcc catgccaaaa ccaatctcaa
    18661 gcgtcaccgg cgcttcacga ccaaaaagcg cagggaaatc cagcatatct tcgctgaact
    18721 caacgcccat caccggccag tagttttcca gcgcatgttc ctggcctttg gtcagtcgcc
    18781 cctggcggcg cacaaaacta cggatacggc gcagcgggcg gccgttttca tcaaattccg
    18841 gtgagatgac gtcgtttttc ataaagattt agtcgcttgt gaaagtgttc tgaaaacggg
    18901 cattatccaa agttagttgc cggatgcaag catgataagg ccgtggctgc ggaaagttcc
    18961 ggtttacacc ctgccgtcgc tgtgctgcaa tcttgccccc aacaacagtg aattcggtga
    19021 ccatgcaagc gtcgcaattt tcagcccagg ttctggactg gtacgataaa tacgggcgga
    19081 aaacgctgcc ctggcaaatt gacaagacgc cctacaaagt atggctctca gaagtgatgt
    19141 tgcaacaaac tcaggttgcg accgttatcc cctattttga acgctttatg gcacgcttcc
    19201 caacggtgac cgatctcgcc aatgcgccgc tcgacgaagt tctccatttg tggaccgggc
    19261 ttggctatta cgcccgcgcg cgcaacctac ataaagctgc acaacaagtg gcggccttac
    19321 acggcggtaa attcccagaa acctttgagg aagttgcagc actgccgggc gtcgggcgtt
    19381 ccaccgcagg cgcgattctc tcgctttctc tgggtaagca ctttccgatt ctcgacggta
    19441 acgtcaaacg cgtgctggcg cgctgctatg ctgtaagcgg ctggcccggg aaaaaagagg
    19501 tcgagaataa actgtggagt ttaagcgagc aggtgacgcc cgcggttggc gtggaacggt
    19561 ttaaccaagc gatgatggat ttgggcgcga tgatttgtac gcgctctaag ccaaaatgct
    19621 cgctctgtcc gctacaaaac ggatgtattg ccgccgccaa caatagctgg tcgctttatc
    19681 cgggcaaaaa accgaaacag acgctgccag agcgcaccgg ctactttttg ctgttacagc
    19741 acgaagatga agtattgctg gcgcagcgtc cgccgagcgg attgtggggc ggtttatact
    19801 gcttcccgca gtttgccgat gaagaaagtt tgcggcagtg gctggcgcaa cggcagattg
    19861 ctgccgataa cctgacgcag ctgaccgcgt ttcggcatac cttcagccat ttccacttag
    19921 atattgtgcc tatgtggctt cccgtgtcgt cattcaccgg ctgcatggat gaagacaatg
    19981 cgctctggta taacttagcg caaccgccgt cagttggcct ggcggctccc gtggagcgtt
    20041 tgttacagca gttacgcact ggcgcgccgg cttagcgcgt gagtcgataa agaggatgat
    20101 ttatgagcag aacgattttt tgtactttcc tgcaacgtga agcagaaggt caggattttc
    20161 agctgtaccc cggcgaactg ggaaaacgca tctataacga gatctccaaa gaagcctggg
    20221 cgcagtggca gcacaaacaa accatgctga ttaatgaaaa gaaactcaac atgatgaatg
    20281 ccgagcaccg caagctgctg gagcaggaga tggtcaactt cctgttcgag ggtaaagagg
    20341 tgcatatcga gggctatacg ccggaagata aaaaataaaa acagtgccgg agcacgcctc
    20401 cggcaacttg cataaaaaca aacacaacac gcacccggaa tgatgaaaaa atatctcgcg
    20461 ctggctttga ttgcgccgtt gctcatctcc tgttcgacga ccaaaaaagg cgatacctat
    20521 aacgaagcct gggtcaaaga taccaacggt tttgatattc tgatggggca atttgcccac
    20581 aatattgaga acatctgggg cttcaaagag gtggtgatcg ccggtcctaa ggactacgtg
    20641 aaatacaccg atcaatatca gacccgcagc cacatcaact tcgatgacgg tacgattact
    20701 atcgaaacca tcgccgggac agaacctgcc gcgcatctgc gccgggcaat tatcaaaacg
    20761 ttgctgatgg gggacgatcc gagttcggtc gatctctatt ccgacgttga tgatattacg
    20821 atttcgaaag aacctttcct ttacggtcag gtggtggaca acaccgggca gccgattcgc
    20881 tgggaaggtc gcgcgagcaa cttcgcggat tatctgctga aaaaccgtct gaagagccgc
    20941 agcaacgggc tgcgtatcat ctacagcgtc accattaaca tggtgccgaa ccaccttgat
    21001 aaacgtgcgc acaaatatct cggcatggtc cgccaggcgt cacgcaaata tggcgttgat
    21061 gagtcgctaa ttctggcaat tatgcagact gaatcttcct ttaacccgta tgcggtcagc
    21121 cgttccgatg cgctgggatt aatgcaggtg gtacaacata ctgccgggaa agatgtgttc
    21181 cgctcgcagg ggaaatccgg cacgccgagc cgcagtttct tgtttgatcc tgccagcaat
    21241 attgataccg gcaccgcgta tctggcgatg ctgaacaacg tttatctcgg cggaattgat
    21301 aacctaacat cgcggcgtta tgccgtcatc accgcctata acggcggcgc aggcagcgtg
    21361 ctgcgagtct tttcgaatga taagattcag gctgccaata ttattaacac catgacgccg
    21421 ggcgatgttt atcagacgct gacgacccgc catccctctg cggaatctcg ccgttatctt
    21481 tataaagtga ataccgcgca aaaatcttac cgccgccgat aattccacta accgcccctg
    21541 acgatgctca ggggtaaaaa tgttatctac atcacaattt cgttttgcaa attgggaatg
    21601 tttgcaatta tttgccacag gtaacaaaaa accagtccgc gaagttgata gaatcgcatc
    21661 atctcgcacg gtcaaatgtg ctttttcaaa cactcatccg catcacgatg tgaggaaatt
    21721 aacatgaatc ttaagctgca gctgaaaatc ctctcttttc tgcagttctg tctgtgggga
    21781 agttggctga cgaccctcgg ctcctatatg tttgttaccc tgaagtttga cggtgcttct
    21841 atcggcgcag tttatagctc actgggtatc gccgcggtct ttatgcctgc gctgctgggg
    21901 attgtggccg acaaatggtt aagtgcgaaa tgggtctatg ccatttgcca caccattggc
    21961 gctatcacgc tgttcatggc ggcagaggtc actacgccgg aggcgatgtt ccttgtgata
    22021 ttgattaact cgtttgctta tatgccaacg cttgggttaa tcaacaccat ctcttactat
    22081 cgcctgcaaa atgccgggat ggatatcgtt actgacttcc cgccaatccg tatctggggc
    22141 accatcggct ttatcatggc aatgtgggtg gtaagcctgt ccggcttcga attaagccac
    22201 atgcagctgt atattggcgc agcgctttcc gccgttctgg ttctgtttac cctgactctg
    22261 ccacatattc cggttgctaa acagcaagcg aatcagagct ggacaaccct gctgggcctc
    22321 gatgcattcg ctctgtttaa aaacaagcgt atggcaatct tcttcatctt ctcaatgctg
    22381 ctgggcgcgg aactgcagat taccaacatg ttcggtaata ccttcctgca cagcttcgac
    22441 aaagatccga tgtttgccag cagcttcatc gtgcagcatg cgtcaatcat catgtcgatt
    22501 tcgcagatct ctgaaaccct gttcattctg accatcccgt tcttcttaag ccgctacggt
    22561 attaagaacg taatgatgat cagtattgtg gcgtggatcc tgcgttttgc tctgtttgct
    22621 tacggcgacc cgactccgtt cggtactgta ctgctggtac tgtcgatgat cgtttacggt
    22681 tgcgcattcg acttcttcaa catctctggt tcggtgtttg tcgaaaaaga agttagcccg
    22741 gcaattcgcg ccagcgcgca ggggatgttc ctgatgatga ccaacggctt cggctgtatc
    22801 ctcggcggca tcgtgagcgg taaagtggtt gagatgtaca cccaaaacgg cattaccgac
    22861 tggcagaccg tatggctgat tttcgcaggt tactccgtgg ttctggcctt cgcgttcatg
    22921 gcgatgttca aatataaaca cgttcgtgtc ccgacaggta cacagacggt tagccactaa
    22981 ttacgcaaag aaaaacgggt cgccagaagg tgacccgttt ttttattctt acttcaacac
    23041 ataaccgtac aaccgtttca tgccatccgc atcggtttcg ctatagacac cttgcaactc
    23101 cggcgaaaat cccggcaaca aattcacgcc ttcttccagc gcaaggaaat aacgttgaac
    23161 cgccccaccc cagacttccc cgggtaccac gcaaagcacg ccaggtggat aaggcaacgc
    23221 cccttctgcc gcaattcgcc cttcggcatc acgaatccgc accaactcca cttcaccgcg
    23281 aatataagcg ctatgcgcat cctggggatt catcaccact gacgggaaac tctgctggcg
    23341 gaacatcgct ttttgtaggt ctttgacgtc gaaactgaca tacagatcgt gcatctcctg
    23401 acacaactgg cgcagggtgt agtcgcgata gcgcaccgga tatttgttat aaacgctcgg
    23461 caacacctca gccagcggcg agtcatcctc aatatgctgt tcaaactgcg ccagcatcgc
    23521 caccagttgt gccagcttct cgtggctttc cgccggagtt aataagaaca gaatggagtt
    23581 gagatcgcac ttctccggca caatgccgtt ctcacgcaga tagtgcgcca gaatcgtcgc
    23641 tggaacgcca aaatcgctat attcgccggt ttcggcatcg atacccggcg tggtgagtaa
    23701 cagcttgcac ggatcaacaa aatactgatc cgcggcatat ccttcaaagc cgtgccactt
    23761 cgcccccggc tcaaaactga aaaaacggcg gtcgctggct aacactgacg tcggataatc
    23821 ctgccacaat ttgccatcaa caacgggcgg gataaacggg cggaacagct tacagcgcgc
    23881 aagaatagcc ttgcgcgact caatccccaa cgcaacgcac tccgcccaca gccgacgccc
    23941 actctcccct tcatgaattt tggcgttaac gtccagcgcg gcgaacagcg gatagaacgg
    24001 gctggtagaa gcatggagca taaaggcatt attcaaccgc ttatgcgggc aaaaacgcgc
    24061 ctgtccacgg atatggttat cttttttatg gatctgcgac gtctgtgaga atcccgcctg
    24121 ctgtttgtgc accgactggg tcacaaagat ccccggatcg ttttcgttaa gttctaacag
    24181 cagcggcgag ctatccgcca tcatcgggat aaactgttcg taaccgaccc acgcggaatc
    24241 aaacagaatg taatcacaca gatgcccaac ggtatcgatc acctgacggg cgttatagac
    24301 agtgccgtca taggttccca gctgaataat cgccaggcga aacgggcgcg gcaggtcggc
    24361 tttttctggc gcaacgtcgc gaatttgctg gcgcagatac tcttcattaa aacagtgcgc
    24421 atcaataccg ccaatgaaac caaacgggtt acgtgaagct tccagataga ccggcgtcgc
    24481 ccccgcctga atcaacgcgc catgatgatt cgacttatgg ttgttacggt cgaagagcac
    24541 cagatcgcca cgcgttaaca gcgcattcgt caccacttta ttcgctgctg atgtgccgtt
    24601 cagcacaaaa taggttttat cggcatgaaa gactttggct gcgaatttct gcgcatcttt
    24661 cgccgatcct tcatgaataa gcagatcgcc caattttacg tcagcgttac acatatcggc
    24721 gcgaaagatg ttctcgccaa agaaatcgta aaaatggcgt ccggcaggat gctttttaaa
    24781 gaacgcacca tgttgatgtc cagggcaagc aaaggtgcta ttacccatct caacgtactg
    24841 cgtcagcgtg tcataaaacg gtggcagcaa attctcttca tactgacagg ctgcggattc
    24901 cagctccagc cactgctgct cgttgccgtt gattaccgcc gtaacgcccg caggtaattc
    24961 aacagcatgt tcggaataca aaaacaccgg tagatgaaaa ccggtgcgct taagcaacgc
    25021 aagaatgcca ctgcgactat ccgcagcggt aatgacgact gccgcgacgt ccgtaaaatc
    25081 agtatctccc aacgccacca cgcggcgatg agtagaaagt cgggatacca gttcactact
    25141 ggcggcaata ttcattgatt tcataagcgc aaaacccgtt tcggggaagt aagaataccg
    25201 gacaaggtgg aaaaccctgc cccatgagat atgggtcaaa ctggtcacca gctccgaccg
    25261 ccagacatca gtaaaagcag aaacgctctg attttactgt tgtcctgcag tgagcgtgcg
    25321 ttaacttcac cgcatgagca gtagcataaa aagagaagag tttcgcgcga aacggcgata
    25381 agcgagagaa tgtaaggagg tggcgtgcat cgggcaaact ccgtacagag aggagaaaat
    25441 tcgcgcaatc atggcacctt tcgctaaagc gtgcaagcca ggactttgcg aacaaacaag
    25501 ccatcgacgg cgccaaaagt tcataataag aaaatcaaac aatacagcta acaggaactc
    25561 ttgtggtcat cggccctttt atcaacgcaa gtgcagtctt actgggtggc gttttcggcg
    25621 cactccttag ccaacgctta ccggaacgta tccgcgtctc catgacatca atttttggtc
    25681 tggcatcgct ggggattggt attttactgg tggtgaaatg cgccaacctt ccggcgatgg
    25741 ttttagcaac cttactcgga gcattaatcg gcgaaatttg tctgctggaa aaaggcgtca
    25801 atacagtggt cgccaaagcg cataatctgt ttcaccactc gcgtaagaag ccagcgcatg
    25861 aatcttttat tcagaatttt gtcgcgatta ttgtcctgtt ttgcgccagc ggcaccggaa
    25921 tcttcggggc gatgaacgaa gggatgaccg gcgatccgag tattttaatc gccaagtcat
    25981 ttcttgattt ctttacggcg atgatcttcg cctgctcgct gggtattgcg gtatcggtga
    26041 ttagcattcc attactgatc atccagttaa cactggcgtg ggctgccgcg ctgatattac
    26101 cgctgaccac accgtcgatg atggcagact tcagcgccgt aggcggttta ctgctgctgg
    26161 caaccggatt acgcatctgt ggcattaaaa tgttcccggt ggtcaacatg cttccagcac
    26221 tccttctggc aatgccgctt tccgccgcat ggaccgcctg gtttgcctga caatgcgtgc
    26281 aatagcggca aagtgatgat agattgtgca gtctgcacta aattgaagaa atttgattga
    26341 cgagacgagg cgaatcaggt ttaatgcgcc ccgttgcccg gatagctcag tcggtagagc
    26401 aggggattga aaatccccgt gtccttggtt cgattccgag tccgggcacc acctttcctt
    26461 ttttatgctt ctctatgaat ctctatatgc atgaaattca gcaggttagc ataaaaatct
    26521 ttcccgatac gttttgattt gttcctatgt atccgggaaa tttggggtca gattcggggt
    26581 ctttcagttc gatgaactaa ggagccccat atggccctga ccgacagtaa aatccgcgct
    26641 gcaaaacctc tcgcaaaatc ttacaagctt actgactcgc aaggtctgta cctgaccgta
    26701 tccaccagcg gttctaagct gtggtatttt cgctatagtt tcggtggcaa agaaagccgt
    26761 ctggcttttg gcccctaccc gcaaataacg ctggcggaag cccgcgaaaa acgcgatgcg
    26821 gcgcgtaagc tactggcacc ccgaatctgc ccttcgcagt tacttaagcc agaaaaagac
    26881 accgttgacg cctcccggac gtttcagttt atcgccactg cgtggcacac cagttgcctt
    26941 aaactctggt cggaaggcca tgcggataag attcttacct gcctgaaacg ctatgttttt
    27001 cctgatattg gcgcgatgga tattgcaaag attgagaccc ggcatctggc acagcttgtg
    27061 aaagcgattg atgataaagg cgtgcatgac gtcgccgggc gggtaaaaca gcatctgact
    27121 aaaatcatgt gtcacgccgt acagcagggg attatcaaat ataacccggc gtatgattta
    27181 gatggcgtcg tgacccctgt tgtgacccag catcaccccg ccctgcccct gaaacgcctg
    27241 ccggagttac tggagaaaac ggacggctat aaaggccggg aactgacccg actggcgctg
    27301 gagctgaatc tgcatgtctt cctccgttcc agtgagctac gccttgcccg ctgggacgag
    27361 tttaacctga aagcgcatat ctggacggtg cctgcgaagc gtgaggcagt gaagggtgtg
    27421 cggtttgcag agcgtggcgc aaagatgaag gatgagcatc tggtgccgct gtcggcgcag
    27481 gcggtggcgc tgctgaaaca gattaagaca atcaccggcg agtcggtttt cgtttttccg
    27541 ggagcgcatt cgctggagaa gccgatgagc gagaacacta tcaacaaggc gctgagggtg
    27601 attggctatg acacaaaaac ggaggtttgc ggtcacggtt tcaggacgat ggcctgtagc
    27661 gccctgaatg agtcggggct gtggtcaaag gacgctatcg agcgacagat gagccacaag
    27721 gagcgcaacg gcgtgcgggc agcgtatgtg cataaggccg aacatctgga agcgcggatg
    27781 gaaatgatgc aatggtggtc ggactatctg gacatgagcc gcgaggggta cgttgcgccg
    27841 tatatttatg cgcggcagca taaggtggcc tgagtcagtg acggcatgaa ttatcggtaa
    27901 tggaaaagca gataccggcg ggtctgcttt tttgtttgcg ccctttttcg cttttagccc
    27961 tttccggtca gagccattcc ccttgaaacc cttgccccga ggccctttcg tgaaaggttg
    28021 cgactgttaa tttcgctgta ttaagaggaa accgtacaga atttttccgg gtggaaattt
    28081 tttgtacggt ttcctcttaa taacgccgga tcaaacggtc aaacctgcca gccagaaggt
    28141 ttgtctattc ctccatgcct cgtccgtaca cctgccggat atagcgcccg cgcccatcgc
    28201 cgtggcccag atcggtggag acctgcgcca gcgcctcttt tttacttaac ccctgcgcca
    28261 gccagtaacg tactgcatcc tgcgcccagg cgtagcgcag gctgtgcggg gagtgctcgc
    28321 ccgtcaggcc gcagcgccgc gcctgattgt gccaataatt catggcggtt ttcagatctg
    28381 tcgcatcgat cagcctgccg ttacgctctg ccgcaatact gattgcccgg tctaccgcct
    28441 gttttaccgc gacggagtcc tgtacggtag tcattcgggg acgtccgccc ttggtgccaa
    28501 ataccacgcg taactgttgc tcaccatttg ccagcgcctg ccgccacgtt ttaagcgact
    28561 gtgcacactg taccgcttcc tgcgagcgta gccccatcag ccgcgccagc gtcagggctg
    28621 ccgcaaaccc cggatcggtc gcctgcgctt tttgcagtgc cgactgataa gcgcgttcag
    28681 gaatggcctg ctttgtgccg ttacggcttg cgccagccag ccctaatgta cgattactga
    28741 gccgtgcatc tgacgccagc ttatcacgtc cggcctgttg cagaacggca cgcagcgccg
    28801 ccatctcgtt ttgcagcgtg cgtttactga taccctgcgt cagccgatgc tgaatgtaac
    28861 cttcgatgtg ccgcgtcctg atatgcgcca cctgtttact ctgaatattc agcgccagaa
    28921 ggtggtgaca gaatcgcttc gcgatcgcca tgcggtcgtg gacggtcttg tggctgccgc
    28981 ccacctgctg tgccaatgtg gtcatttgct gcgccagttt tcgcattgtt tctcccgttt
    29041 ttgtaagaaa atgactgttt tccccggtgc ggcggtagta ggccctctgg ccggaacgat
    29101 atctgtgcgc cggggcgaca gcggttgagg tatgacgggg aacggttgga cattcaatgc
    29161 cgccgcctgt gttaacaggt aatcccccgg atcgggcgat ccgccttgat atcattcatt
    29221 tcaggccgtc actggccggt ttcctgctat cctgtgggtg aattttgatg gtgtggttct
    29281 ccttgtgagt gcgtcagatt gttgccgccg ggtgggatcg gcgtgggtaa aaggctgtcg
    29341 tggcgtaggg tcgcgacgtc actttgttgc tttgcgcaac gaaagtgacg cgcaggtccg
    29401 gtggattgtt ccgtcacgcg gctgcaaagc cttacggcaa tgcaggccgc agccggaacg
    29461 ggtgtgatct ggccctgacc gcaaagcggc ccggtatttt caaaggcatt accagtgtcg
    29521 ggtgctgtca acgacagcgt gttgcaggcg ggaggttacg gcgttgtcag tgacgcagga
    29581 aggagaaacc ggatgtggcg tttaaaatat tttctcccga atttccgcca gcaaagctgg
    29641 cagaaattca ggagagcctg ttttactgct attcaggctt acggtagcgg gaaggccaga
    29701 tttgctccgg cgcaacgcct aattcttcgg cgattaatcg ttcacctttg ggccagtgac
    29761 gacagatcgc gtttgccagc gtggaagatg ccagcccagc cctgcgggaa accgccgcca
    29821 gcgatgtacc gcgttttcgc agcgccgcca tgatatcagc gggatgccag tccggttgcg
    29881 tcatacagtc acctcaccga taaatttcac gccgtcggcg gtgcgcagcc agcgcggggc
    29941 tttgagtcct tcggtgaaat accagaccag ttcacataac agcccggtca gggtttgttc
    30001 cgttttgggt tccgctgggc gttccagcag aagccggagc agcgtgaggc agtactcgca
    30061 cagggaatca ggttcagaaa caaagtgctg tggtatcgat ggcgggacgt caacggtcag
    30121 gctttcaaca agatgcggcg ggatggggtc gtgcagcgtg ggttgcagca gcgccaggca
    30181 ggcgtgaagc cgtccgcaga gtgccagccg tagcgcagga tcgtcggttt ctatcagggt
    30241 ttcggcgaaa cgggcgcaat agtcggcaag gacggtgaaa tccgtggtgg cgttaaaggg
    30301 aacggacaac aaatcgagta aatcgcagtc aggggtgagg attgtagcca ttagggcaac
    30361 ctccggtatg tgaatttaaa ttccaccacc gaagagacca atcttacggg tggtgaactg
    30421 aacggagttg gtcttaccgg ccatacctac accggcgcac cttgcggtgc ccccgcccag
    30481 cccaccatag agatgtagcc gaacgcacga cataaaaaaa gcgcggatgc gccattatgt
    30541 cgcaggtatg atattcagga gaccaatccc gacgcctaat tttgcaggcg cgggggaagg
    30601 atactgcgat tggttttgcc agacaaggaa tttgtcgggg gaaccaatta cacttctctg
    30661 agcaataatg ccagcgcaaa acagtgacta aattcgctcc aatacaaaaa cacgaccatc
    30721 tcaattaatt gttatccaaa gcaaagattg aatcatcaga tgaaatcgcc tacgatagcg
    30781 aaagccatcc cccgggatcc ttagcctcta cacgatagtc acgctcgtac gaggtgtacg
    30841 gggctcgccg acagccagtc ggcgtcgaaa gaaaggagcc cggtatgaaa cagaaaactg
    30901 tgctggttca ccagtatctg cgtttccgtt ttggtcgctg ggagcacgtg tgtcagcaca
    30961 tgcgctcttt accgaactgc taacggttaa tcccggggtg atggtcctct tctattgtta
    31021 atcaggccgc ttgtgtcgtc ttttccatca ttgatgtgta atgttcttca tctttcgccg
    31081 cctcgaatac tttcttaaac caggcaagat agcgttcaac agaagccccc tcatgaagag
    31141 agatattgcg ggcatcctgg tgagaatgtc ggtttaggta acgagcaaat atacgaaaac
    31201 ctggttcacc ctctggtccg ctagccagtg tttcaagcgc ccggtgaagg gtttcacttt
    31261 tccccgaaaa accaaagtag tattcaagga tttgccgcat cgtatttggg atcaccacac
    31321 tggcaatgtc atctttctgg gcgtggcgta agacatacca tagtgcctga tagtcgttca
    31381 gtaattcttt ttcggaaaca agagaagcag aactgtgcag ggatttggat atccgataaa
    31441 gcgtccattc tggtgggcaa gaacccgcag cccttttctt tctttccgcg gaaagcagta
    31501 actcctgaaa gaaaaacagg ctatgcgtca gaataataac tttctcagca attttggcac
    31561 gtataacctg atgctggatc aacgaagcaa tttcaaaaac atagttttgc gacagactgg
    31621 atatcggatc atcaataacg attaagcgtt tccgctgatc atcgtcatcg cgactttttc
    31681 ttccggtaca ggtttccaga aaatagagaa aggcaatcag cgttttttca ccttcactca
    31741 ggctactaaa aacaggcaca tcgcttttct cacccgaccg caccagacga taatcatccc
    31801 ggtcgtcata ggaagcaatg tggaacccag tgatcccaag aatattcaaa ccatgattaa
    31861 ttcgatcaat cgtttcctgc gtattgacga tctggctgga tttatcacga atagttgcgg
    31921 tcagtgcact aagctgagcc gaaatttctt tctgagatac cgttattttt tctgattttt
    31981 cagcaagttt cgctagttgt tcatcatggt taattatcgt atctgtagcc agctttcgga
    32041 tatgaccgag tagtttctgt ttcagccctt ctcgctcagt ctgataattc tcagcctttc
    32101 ggttatgctc actgattgcc acattaatac tggcgatctg tgcagaaaca ttgcctgcca
    32161 gtgcagaaag atcctcaaga gagatcgatg cggagggtga tgttaatttc tcatcgatat
    32221 gcattttatt tcgttggtac gcctgctcca gttcaagcaa acacagatat acaggatgct
    32281 ccagatcaac caacggacat gcttttatta actggcgcat atgttcaggt ttatcgtgcc
    32341 agagttgata tcttccctga agttcccgca actgatccat cgcgatttcc catgaggtat
    32401 caaaaagggc ggtgatttcc tgttgaagtt gtcgggcatc tgtcggcgtt tgacagaatg
    32461 ggcaaatatc atcaatcagc cacttctggc cgctcctcac ccagtcaatg ttgcctaatt
    32521 ggttaattgc tgcggcaagg cgactatcac cggctggaat aagaggttgc atcaatagcg
    32581 caatatcact ttcattcaaa ggggatgtag gcaatgcctc aagcaaaggg taaggattgc
    32641 ctttgctggc ctcaagtcgg ttcagctctt cggttagttc ctgcgtcgag gtttcttcta
    32701 ctttcaggtg gccaaggatg gtctgataaa gtgggttggt ctgcttggct ccaaccatca
    32761 attcccataa atctgttttt ctaatctctg cggtggaact ataaatctgt tttgcagcgt
    32821 cactcttcac gctctcttcc atgccctcaa catcacattt ttgtacgtcc agagcgtcac
    32881 gccggtccaa catctcttta ctctctgctt gcagagcatc tatttccgtt tttatttctg
    32941 cgttttcact atttaaggta aaaacgccag gctgagaagg ttgactgaaa atatcgtcta
    33001 catactcctg attaaacacc aggtagttca tattcagcgg aggttgcagg ttgcactgac
    33061 tgtaatctgg tcggccatgc ttataaaagt aaccagaaac ggttgatttc cctgaaccgt
    33121 tttgtccata gataagagtg acatacttac tcaagtcgaa ctccactggc ctttcgccct
    33181 gataactgcg aacaccatgg atggagagaa tgctcatgct atatccttat caccgcctgc
    33241 tgtatgtggg tattttatcg ccggaattcc ctacagcaag ccacacaacg aacagttacc
    33301 agaaaaaata agtatcttac aataaacatt actgacatga tcatgattag actccttgct
    33361 atataaggga tataatgaaa tcacatacta ttaaagacgt cagcacagga gaatcaatgt
    33421 ctgttgccgc tctggcccgc cagcgggtcc accgcatgaa gcggggtatc cccttttcca
    33481 tcagcggatt ttattcgctg ggaagccgtg cgtcggtaca acaggcaatg agtcgtctgg
    33541 tcaaagaagg tctgctggtc cgggttgaac gcggtttcta ctcccggcca aaaccgttaa
    33601 aaaaccttcc atccgtaaat gttgtcgcta gtgccaatga ggttgccgcc gcatgggcaa
    33661 aagaacgcgg ttacaaaatc acccttcagg gactggaagc cgcttaccgg ttagggctgc
    33721 aaacgcaggc accggttaaa cgcatctact ggtcaaacgg ccctacgcga gagttccgtg
    33781 tcggtaatga gttggtacgg atccagcata aatcagcaag taggttacgt tggctgaacc
    33841 ggcctgaagg agagctgatg cgcggtttgc tgagtctgga tcagcaacat accccatcgg
    33901 aaacgctgaa gaatgctttt catcggctga gcatcccgtc cgctaaagcg cgacagattg
    33961 caggaaagtt aatgaatgag cctgcgcttc acgcgtggcg ctcgcagctt atagcgttgc
    34021 aaaaggcgct ctgatgaaca tttttgatta ctaccggaac gcatcgcaac gccgtgagct
    34081 tcaggaaatc ctctcttacg cggcgcagca gcatccctcc gggctgggag ccagcttcct
    34141 ggaaaaggat ctctgggtaa cggagatcct tcggctgctt tttaatgagg atctgctggg
    34201 agatttatct gtcgcgttta aaggtggaac ggcattaagc aagtgctgga acgtgattga
    34261 gcgcttttct gaagatatcg atctttctgt tcactgggcc gatctggcag gccactcaga
    34321 agaggaggaa cagcaggcat gggaacaaag cacgctgaat aatagccaga atcgccggtt
    34381 cagagagcag cagcaagagc gcttaacaac atggacaagc gcgctggcag aacggttaaa
    34441 ccaacgcttt caggcttatg aaattccggg ccttgaagca aagatggaac ccgacagcag
    34501 tggcgaaaaa atagacatca atttcccgcg ggtgaaagaa gacaatatgg cctatcagcg
    34561 cgatcatatt ctgctggaat ttggcggacg aaatcgtggc aagccaaccg atctaatgcc
    34621 tgttgtgagc tatctgtccg ggattgctgg aatgaatgca cttctgttac ccgccgcgac
    34681 cgtaaacgct tatgacaccg gttatatttt ttgggaaaag ctgacggcgc tgcaccagtt
    34741 ctgcacgcaa accaaagcac caaatccggc ccgactggca cgccactggt atgatgtaga
    34801 ttgtctgctg cgtaataatt tcgccagccc atatgaaacg caggaggcca tgcggaatgt
    34861 ggttgagatg aagcaacgcc gttggtctgt gccgggcgtg gattttactc aggtgattac
    34921 cggaaacttg caactgttgc ctgacgacgc cgaacggttc gccgcgatcg ccggggatca
    34981 tcaaacggcg gtggaagggg gaatgttctt tcgtgagcct gacggatttg ctgacattgc
    35041 cgatcgtctt cgtcatgtac aagatgaaat caatcgtttt atgtacggtt catagatata
    35101 aactattcct actaattaat ccctcagcca tatgacataa gccttttaaa taatagggct
    35161 tttatgcttg tactccagcc tattcgcggt atcattttac gatctctctt ccagttttat
    35221 gcttaccgcc tttgctgctc gttgaacaat gccgggaagc gttatcagcg attaagggca
    35281 cggaatgggc ttctggatat tcgttattgc actggctgtt atctggcttc tgttttccaa
    35341 aaagaaaaaa tcgcctcccc ccaatataaa cagcaaaatc atcaccaaaa caaattattc
    35401 atcccgacaa gaatctctca ataagccaga cagcaacgtg acaaatatgc attctcagga
    35461 atccgatgat gacgaactgg taacctttag ctttgttaat ggacaggcga ttgaatacag
    35521 caccagccgt cagccatcgc gagaaagcgc cgcacgtagc aataccacgc cagcgcgatg
    35581 gatcaaaccg ggagaaagta tcatcattca aaacatcgtc attaagaacg gtaattttta
    35641 ttttggtggg cagttaaaaa cacattcatc aggcgaatat agatatcttt ataacgatgg
    35701 ttccgatgct tctctggtta atgactcttt atccattgag cctagttcac gccactatta
    35761 tgatgaatct ctgggatact ggcccacctt tgctactctt tctcctcgct gtcgtggcgc
    35821 ctatcttgac tggctggcaa gcgatcgcag cgatgcgagc tgccccgttg gttatgtgtt
    35881 tatctatttt tacggtcttg aacgccgcgt actagcggat ggcacacaag aggctatttc
    35941 tgacgatgaa ttcaaagcat tattcgaaga gatatctcgc ctgagaatcg tatttcaggc
    36001 aagcggttcc ttccgtcatt atgcaacaca gttgctggaa atgatgctca ttctcagacc
    36061 aaagttactt tctatacata ccgaaaacga atatttctca tcgaggagtg cattactgtt
    36121 cagactaaat ctggcgacgg tggttgataa aggacaacct gtttgcgccg aactggcgct
    36181 ggcatggata cactattttc ctgattacac attacgcacg cctgcccgtc gatgtcatgc
    36241 tgaattttcc gcgttattca aacagcgtta tacccgaaaa tacggtgacg gtattgtcgt
    36301 caaacccaat aaaacacggt tgtatttaag ctataccccc gccagtggta cgcttcggga
    36361 acttcaggta aaaaacagat ggttcttccc gatcccagcg ttttaaaagc cccagttcag
    36421 aaattaatcc ctgttgcaga atcctgtgtc aatgctctgg atgcctacag tcgctatctc
    36481 ggtaaaaaag atgtctcagc aagcgacgtc gccgccatca tgctgcttcc cgatgaaata
    36541 ctgaccgaag atgcaggacg tctgtttgct gaatttaaac actgggcaga tgagaaaatc
    36601 cgtgaacatt caggactggc gacagtggct gatttctggg ccagactggg tatgctggta
    36661 ccggataaga ttaataagaa agaagccgaa ctgatgcaaa atttcgccag acgagcaggt
    36721 tacggcattg cgccggatat gcgctatgaa ctgaacccca aaacctggac agtttcagtt
    36781 aaccggcttt ttgatactca gtccggtatt ctaccgggct gagtccccca agactgagtt
    36841 ttattctttt ttgattccag taagttatgt attcgttaac ggcattttcc agctcttcta
    36901 cacttctgta gtcccggcga tagtacattt cttctttcag gtgaccaaag aaattctcca
    36961 ttaccgcatt atcaagacag ttgcccttgc gcgacatgct ctgcactaac cctttgtctg
    37021 ccagggcagc ctgataactt tttatccggt aatgccaccc ttgatcactg tggagcagcg
    37081 gtttttctcc ttctgcaagg ctttccagcc ccttatttaa catccgcttc accagctcct
    37141 ctgtaggcct gcaggcagtt tcccacgcca cgatttcccc gttaaacaaa tcaagtatcg
    37201 gggacaggta cagtttttgt ccgccagccc tgaattcggt gatatctgtc acccatttct
    37261 cacagggagc ttccgctttg aactgccgct gaaggatatt ttctgcagcg agtcccatat
    37321 ttccccggta agaccggtat ttcttcaggc gcacaggaga cttcaggccg agctgttgca
    37381 tcagttttcg cactgttttg ccactgaact taagacctct tttctgaagc tcacaatgga
    37441 tccgccggta cccataacat ccccggtgtt catggaagat ggaggctatg agctgtttca
    37501 catcagcata cttgtcctta gccttctgca gtgacagctg gtagtacagc gtgcttctgg
    37561 ccagccctgc agcctttaac aggtcagact ggcagtgtcc gcacctcagg ctccggatga
    37621 ctttcgtttt ttctccagct cctggcgctt tttttccagg agatgctcct gcatggcttt
    37681 cagatatgca ttttctgcgc gaagataacg cagctcattc ttcatctctt ccggcgtcat
    37741 gtttgcctcg ttttcatggg tatcatcaga acgggtcatg cttttctcct tccagcgcct
    37801 gataaaggcc tcattaccag atttgcggta gccttttatc cagtttttaa ccgtgttgtg
    37861 gctgggaatg ttaaaccggg cagctacccg gggtagagac tcagaatgcc cgagagcata
    37921 aagaaccacg caaagtttat ctt
//
LOCUS       DAZFJL010000028        36283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFJL010000028 DAZFJL010000000
VERSION     DAZFJL010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.028"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(134..274)
                     /gene="yjjY"
                     /locus_tag="ACHJ8C_001441"
     CDS             complement(134..274)
                     /gene="yjjY"
                     /locus_tag="ACHJ8C_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO5551324.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AI"
     gene            370..1086
                     /gene="arcA"
                     /locus_tag="ACHJ8C_001442"
     CDS             370..1086
                     /gene="arcA"
                     /locus_tag="ACHJ8C_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO5551325.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1146..2498)
                     /gene="creD"
                     /locus_tag="ACHJ8C_001443"
     CDS             complement(1146..2498)
                     /gene="creD"
                     /locus_tag="ACHJ8C_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO5551326.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDV
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNVALNLSGTGDLSVVPGGRYS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(2556..3980)
                     /gene="creC"
                     /locus_tag="ACHJ8C_001444"
     CDS             complement(2556..3980)
                     /gene="creC"
                     /locus_tag="ACHJ8C_001444"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO5551327.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEINVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVDQEYVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGMLASLRLHRHFT"
     gene            complement(3980..4669)
                     /gene="creB"
                     /locus_tag="ACHJ8C_001445"
     CDS             complement(3980..4669)
                     /gene="creB"
                     /locus_tag="ACHJ8C_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO5551328.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFDVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4682..5155)
                     /gene="creA"
                     /locus_tag="ACHJ8C_001446"
     CDS             complement(4682..5155)
                     /gene="creA"
                     /locus_tag="ACHJ8C_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO5551329.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5366..6235
                     /gene="robA"
                     /locus_tag="ACHJ8C_001447"
     CDS             5366..6235
                     /gene="robA"
                     /locus_tag="ACHJ8C_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO5551330.1"
                     /translation="MDQAGIIRDLLIWLEGHLDHPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNSPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6232..6879)
                     /gene="gpmB"
                     /locus_tag="ACHJ8C_001448"
     CDS             complement(6232..6879)
                     /gene="gpmB"
                     /locus_tag="ACHJ8C_001448"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO5551331.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLETRNIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6931..7458
                     /gene="yjjX"
                     /locus_tag="ACHJ8C_001449"
     CDS             6931..7458
                     /gene="yjjX"
                     /locus_tag="ACHJ8C_001449"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO5551332.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWAVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRTSVYHQAVIL
                     ALSPFHNAVYQPLQA"
     gene            complement(7531..7857)
                     /gene="trpR"
                     /locus_tag="ACHJ8C_001450"
     CDS             complement(7531..7857)
                     /gene="trpR"
                     /locus_tag="ACHJ8C_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO5551333.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLED
                     VLLKSD"
     gene            complement(7947..9884)
                     /gene="sltY"
                     /locus_tag="ACHJ8C_001451"
     CDS             complement(7947..9884)
                     /gene="sltY"
                     /locus_tag="ACHJ8C_001451"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO5551334.1"
                     /translation="MEKAKYVTWRLLAVGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10101..11768
                     /gene="ettA"
                     /locus_tag="ACHJ8C_001452"
     CDS             10101..11768
                     /gene="ettA"
                     /locus_tag="ACHJ8C_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO5551335.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDSLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11824..12108)
                     /gene="nadS"
                     /locus_tag="ACHJ8C_001453"
     CDS             complement(11824..12108)
                     /gene="nadS"
                     /locus_tag="ACHJ8C_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO5551336.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKEPARVTRYEIADVKAIREQLNV
                     SQSEMAKALGTSVDTIKSWESKRRNPTGLAAKVLATIKENPAFFRELASH"
     gene            complement(12110..12442)
                     /locus_tag="ACHJ8C_001454"
     CDS             complement(12110..12442)
                     /locus_tag="ACHJ8C_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5551337.1"
                     /translation="MGKAIIFIETPMFTRQIKQIATDDELKELQKELIGTPDKGDLIQ
                     QTGGLRKVRMAAGSQGKSGSVRIIYFLATEEIIYLIMAYPKNAKDSLTDTEKAQLKKL
                     TKLLKGEI"
     gene            complement(12534..13766)
                     /gene="nadR"
                     /locus_tag="ACHJ8C_001455"
     CDS             complement(12534..13766)
                     /gene="nadR"
                     /locus_tag="ACHJ8C_001455"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO5551338.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRYLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13787..15169)
                     /gene="radA"
                     /locus_tag="ACHJ8C_001456"
     CDS             complement(13787..15169)
                     /gene="radA"
                     /locus_tag="ACHJ8C_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO5551339.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(15218..16186)
                     /gene="serB"
                     /locus_tag="ACHJ8C_001457"
     CDS             complement(15218..16186)
                     /gene="serB"
                     /locus_tag="ACHJ8C_001457"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO5551340.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGERVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            16292..16936
                     /locus_tag="ACHJ8C_001458"
     CDS             16292..16936
                     /locus_tag="ACHJ8C_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO5551341.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16964..17980
                     /gene="lplA"
                     /locus_tag="ACHJ8C_001459"
     CDS             16964..17980
                     /gene="lplA"
                     /locus_tag="ACHJ8C_001459"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO5551342.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNSDLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            18012..18275
                     /locus_tag="ACHJ8C_001460"
     CDS             18012..18275
                     /locus_tag="ACHJ8C_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5551343.1"
                     /translation="MIDLENQEREIINLMFSQGISWLTAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAGIYGCPPELLICPSWMTAEHK"
     gene            complement(18436..19155)
                     /gene="deoD"
                     /locus_tag="ACHJ8C_001461"
     CDS             complement(18436..19155)
                     /gene="deoD"
                     /locus_tag="ACHJ8C_001461"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO5551344.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(19212..20435)
                     /gene="deoB"
                     /locus_tag="ACHJ8C_001462"
     CDS             complement(19212..20435)
                     /gene="deoB"
                     /locus_tag="ACHJ8C_001462"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO5551345.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(20487..21809)
                     /gene="deoA"
                     /locus_tag="ACHJ8C_001463"
     CDS             complement(20487..21809)
                     /gene="deoA"
                     /locus_tag="ACHJ8C_001463"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO5551346.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLQLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKSVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(21887..22666)
                     /gene="deoC"
                     /locus_tag="ACHJ8C_001464"
     CDS             complement(21887..22666)
                     /gene="deoC"
                     /locus_tag="ACHJ8C_001464"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO5551347.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIDIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            22924..24474
                     /locus_tag="ACHJ8C_001465"
     CDS             22924..24474
                     /locus_tag="ACHJ8C_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO5551348.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPDITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            24446..25309
                     /locus_tag="ACHJ8C_001466"
     CDS             24446..25309
                     /locus_tag="ACHJ8C_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO5551349.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVAWSAAVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LNHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLCHLTCLMDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAALIKGLGDVPVRLNAFHAHGVYGEAQSWPSATPEDVEQLADALWERG
                     VSRLIFPALYL"
     gene            complement(25526..26305)
                     /locus_tag="ACHJ8C_001467"
     CDS             complement(25526..26305)
                     /locus_tag="ACHJ8C_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5551350.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIIPATEAE
                     NFARVLALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPTKMVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRSGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYTLFNVP"
     gene            complement(26302..27375)
                     /locus_tag="ACHJ8C_001468"
     CDS             complement(26302..27375)
                     /locus_tag="ACHJ8C_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO5551351.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(27497..27658)
                     /locus_tag="ACHJ8C_001469"
     CDS             complement(27497..27658)
                     /locus_tag="ACHJ8C_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO5551352.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(27785..28390)
                     /gene="osmY"
                     /locus_tag="ACHJ8C_001470"
     CDS             complement(27785..28390)
                     /gene="osmY"
                     /locus_tag="ACHJ8C_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO5551353.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(28783..30372)
                     /gene="prfC"
                     /locus_tag="ACHJ8C_001471"
     CDS             complement(28783..30372)
                     /gene="prfC"
                     /locus_tag="ACHJ8C_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO5551354.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(30463..31140)
                     /gene="yjjG"
                     /locus_tag="ACHJ8C_001472"
     CDS             complement(30463..31140)
                     /gene="yjjG"
                     /locus_tag="ACHJ8C_001472"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO5551355.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(31155..31601)
                     /gene="rimI"
                     /locus_tag="ACHJ8C_001473"
     CDS             complement(31155..31601)
                     /gene="rimI"
                     /locus_tag="ACHJ8C_001473"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO5551356.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(31570..31983)
                     /gene="holD"
                     /locus_tag="ACHJ8C_001474"
     CDS             complement(31570..31983)
                     /gene="holD"
                     /locus_tag="ACHJ8C_001474"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO5551357.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPIVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            32086..33117
                     /gene="rsmC"
                     /locus_tag="ACHJ8C_001475"
     CDS             32086..33117
                     /gene="rsmC"
                     /locus_tag="ACHJ8C_001475"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO5551358.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLSDYAPLNKMDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            33317..33403
                     /locus_tag="ACHJ8C_001476"
     tRNA            33317..33403
                     /locus_tag="ACHJ8C_001476"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33351..33353,aa:Leu,seq:cag)
     gene            complement(33442..33678)
                     /locus_tag="ACHJ8C_001477"
     CDS             complement(33442..33678)
                     /locus_tag="ACHJ8C_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO5551359.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
     gene            33819..34607
                     /gene="fhuF"
                     /locus_tag="ACHJ8C_001478"
     CDS             33819..34607
                     /gene="fhuF"
                     /locus_tag="ACHJ8C_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO5551360.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPLMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATLHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRYALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(34645..35322)
                     /gene="bglJ"
                     /locus_tag="ACHJ8C_001479"
     CDS             complement(34645..35322)
                     /gene="bglJ"
                     /locus_tag="ACHJ8C_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO5551361.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSARREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(35280..36005)
                     /locus_tag="ACHJ8C_001480"
     CDS             complement(35280..36005)
                     /locus_tag="ACHJ8C_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5551362.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHSERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
BASE COUNT         8684 a         9833 c         9079 g         8687 t
ORIGIN      
        1 gattattaaa ataatgtgac gaaagctagc atttagatac gatcatttca tcaaactgtt
       61 aacgtgctac aattgaactt gatatatgtc aacgaagcgt agttttattg ggtgtcctgc
      121 ccctcttagc ctgttatatt gctgttaaaa tggttaggat gacagccgtt tttgacactg
      181 tcgggtcctg agggaaagta cccacgacca agctaatgat gttgttgacg ttgatggaaa
      241 gtgcatcaag aacgcaatta cgtactttag tcatgttacg ccgatcatgt taatttgcag
      301 catgcatcag gcaggtcagg gacttttgta cttcctgttt cgatttagtt ggcaatttag
      361 gtagcaaaca tgcagacccc gcacattctt atcgttgaag acgagttggt aacacgcaac
      421 acgttgaaaa gtattttcga agcggaaggc tatgatgttt tcgaagcgac agatggcgcg
      481 gaaatgcatc agatcctctc tgaatatgac atcaacctgg tgatcatgga tatcaatctg
      541 ccgggtaaga acggtcttct gttagcgcgt gaactgcgcg agcaggcgaa tgttgcgttg
      601 atgttcctga ctggccgtga caacgaagtc gataaaattc tcggcctcga aatcggtgca
      661 gatgactaca tcaccaaacc gttcaacccg cgtgaactga ctattcgtgc acgcaacctg
      721 ctgtcccgta ccatgaatct gggtactgtc agcgaagaac gtcgtagcgt tgaaagctac
      781 aagttcaatg gttgggaact ggacatcaac agccgttctt tgatcggccc tgatggcgag
      841 cagtacaagc tgccgcgcag cgagttccgc gccatgcttc acttctgtga aaacccaggc
      901 aaaattcagt cccgtgctga actgctgaag aaaatgaccg gccgtgagct gaaaccgcac
      961 gaccgtactg tagacgtgac gatccgccgt attcgtaaac atttcgaatc tacgccggat
     1021 acgccggaaa tcatcgccac cattcacggt gaaggttatc gcttctgcgg tgatctggaa
     1081 gattaatcgg ctttaccacc gtcaataaaa acggcgcttt ttagcgccgt ttttattttt
     1141 ctaccttatt tccagatacg taattcatcg tccgttgtaa cttctttact ggctttcatt
     1201 ttcggcagtg aaaacgcata ccagtcgata ttacgggtca caaacatcat gccggccagc
     1261 gccaccacca gcacactggt tcccaacaac agcgcgctat cggcagagtt gagcagtccc
     1321 cacatcacac catccagcaa caacagcgcg agggtaaaca acatgctatt acgccagcct
     1381 ttcaataccg cctgcaaata aataccgttc attaacgccc caatcagact ggcgattatc
     1441 catgccacag taaaaccggt atgttcagaa agcgccagca agagcaaata aaacatcacc
     1501 aatgaaagcc ccaccagcaa atattgcatt gggtgtaaac gttgcgcggt gagcgtttca
     1561 aaaacaaaga atgccataaa agtcagcgca atcagcagaa tggcgtactt agtcgcccgg
     1621 tccgttaatt ggtattgatc ggctggcgtc gtcactgcga cgctaaacgc ggggaagttt
     1681 tcccagccgg tatcattgcc tgaagcaaaa cgctcaccga gattattagc aaaccagcta
     1741 ctttgccatt gcgcttgaaa acctgactcg ctaacttccc gtttggctgg tagaaaatca
     1801 cctaaaaaac tgggatgcgg ccagttgctg gttaaggtca tttcgctata acgcccgcca
     1861 ggcaccacag aaagatcgcc ggtaccgctt aaattcagcg ccacattcag cttcaggttc
     1921 tgcttgcgcc agtccccttc gggtaaaggg atatgcacgc cctgcccgcc ttgctctaac
     1981 ccggtgccgg gttcaatggt cagcgccgta ccgttaactt caggcgcttt caccacacca
     2041 ataccacgcg catccccgac gctaatcaca ataaatggct tgcctaaggt gatatttggc
     2101 gcgtcgagtt cgctaagacg cgaaacatcg aaatcggctt ttagcgttaa atcactgtgc
     2161 cagacctgac cggtatagat gcctatcttg cgttcttcca cattctgatt gccatcaacc
     2221 atcaatgact caggtaacca aaaatgaata aaacttcgtt tccgctccac ggttttatcg
     2281 tcttcctgca ccgtataaag ctcggtcaca ggaatagcga tgagcggccc aacgagtttt
     2341 tgcggcccgc tggtactttg gcgaatcaca tcttccacat cgctacggta atcggcacgt
     2401 tcgacaatca cctgccgaat cagcattatc ggaatcaaca acagcaatac tgcaccaaac
     2461 aaggtggtca ttttccagaa caggggggat ttcaacatag tcttctcctt tgcaatgaca
     2521 gcaggatacg aaggctatgt gggaagaatt tgaagctatg tgaagtgacg gtgaagtcga
     2581 agcgaggcca gcatgccacc ttcctgcacg ttgcgcagcg tgacttcgcc gttaaacaaa
     2641 cgggcgacct cactgacaaa cgccaacccc agaccgctgc ttttttgccc atttgcacgc
     2701 ggcaaagagt aaaagcgttc aaaaatacgt gacagcgcgt agtcaggaat cccactaccg
     2761 gtatccagca ccttaagggt gacgtattcc tgatccactt cggcgcttag cgttatgcaa
     2821 ccgctctcgg gagtaaaatc gatggcgtta tccagtaaat tccccagcgc ctgctccagt
     2881 aacgccggtt cagcagcaac gttaatctca gtaggcataa catgcaaagt gatgtttttt
     2941 tctgccaact gcacggtgcg cgcttcgctg acgcggcgaa ataatgccgc cacatcaaca
     3001 gcagtcagaa cgacttcctg acgattctcc agtcttgcct ggcgtagtaa cgtttccacc
     3061 agtgcctgca ttcgcgcatt ttgcgtcaga atgttgtcgg taaaacgagc caccacttcc
     3121 ggcggcggac cttcgcgtaa aatttccgcc gcgccacgaa tcgccgccag tgggcttttt
     3181 agctcatgag ttaacgcata aacatactgc tcaatatagt ttttcccttc cagcttcacg
     3241 cgcatacttt ccagcgcctg cgcgagttta cgcaactcgc tactaccgag atcggggaga
     3301 ggaacgggct tattgtcagt gacggaatca gcatagcgag tgagccgggc aatagagcgg
     3361 ttgatccacc aaaccatgcc tgcgccaatc accagtgcaa tccccaacaa aatggcgctg
     3421 gcccataaaa ttcgccgctc gctacgctta atgaccggag ccatcgccgc gttcggtttg
     3481 cctacgctca aaacgccaat aagccgcgag ccgtccataa tcggcgcggc aacatacatc
     3541 acagaacttt cgggatcggc aggattttgc aacgtgctgc gcgcaccata ctgaccacgc
     3601 aacgttagcc agacgtcatt ccagcgcgaa taatcctgtc caacggcttt atttgccgaa
     3661 tcgaacaata ctttgccctg cgcatcggtc atatagacat gatattcgtt gcgcactttg
     3721 ttaatgccac cgatattggc gcgaaacggg cgatgttgta gctgattaaa cgcctgcgcc
     3781 agttgcccat gcgttgggtc cccagagagc aaatcgggac gcgccagctc cgccagcaac
     3841 gttgcggtgt cgattaacgt cccctcggtt gctcttcgca cgcccggttt aacttctttg
     3901 acaaaaatag ccagtacgaa ccaggccgcc accgccacca gtaaaaaata gcccagcaac
     3961 aaccgcatgc cgatacgcat tacaggcccc tcaggctata tcccatgccg cgatgagtat
     4021 taatcggtga aagatcgggg ttgatggcgc gcagcttggc acgcagcgtt ttaatgtggg
     4081 tatcgacggt gcgatcgtag gtgtcctgcg catcttccca cacgctatcc atcagttgct
     4141 ggcgggacca gacgcggccc ggcgacttga gtaacgtctt cagcaataaa aactcatacc
     4201 gcgtcagcgt taatggcgtg tcaaaccagc tgatctgcgc cgcaggttca ttcaattcaa
     4261 aatgaccaat acggataacg ggagacggcg tcgagaactt cttcacccga cgcagtaagg
     4321 tgcgcactct ggcgcacact tcgcgaggtg aaaacggttt agccacgtag tcgtcagcgc
     4381 caatttccag cccaagcagg cgatcgacct cttcacttcg ggccgtcagg aacagtacag
     4441 gtaacgccgg atggagcgcc agtaactggc ggcacaattc aaagccgctg atatcaggca
     4501 aaccaacatc gagaatcatg acatcgggaa cctgctggcg agctttatcc agcactggca
     4561 agcctcgctc aaagacctcg acgtcaaaac cttcctgttg caacatgtag accagcgtat
     4621 cggctatccc ttgctcgtct tcgactaacc agaccgtttc ccgttgcata aatcgcctct
     4681 gttattgccg ccacggcatg acaggaaccg cgctaatcgc gtttttcggc gaaccttcta
     4741 caactttgtc ggagtaagcc agataagcga gcgcgttgcg tttagcgtca taaaagcgca
     4801 cgacctgtaa cgacttaaag accagggacg tgcgtttttt gaatactacc tcgccctgag
     4861 ctttgccgtt tttaatacga tccgacagtt caatcggccc gacttgttga caagaaatgg
     4921 ccgcatcgga ggtatcttct gccaggccca atcccccttt aataccaccg gttttcgccc
     4981 ggctcacata acaggtgaca tttttcacat cgggatcgtc aaaggcttca acaacaattt
     5041 tgtgatccgg gccgatcatt ttaaatacgg tgtcgaccga accaatctct tctgcatgag
     5101 ccaatggccc cagcattatc aggcttaaag acaggattaa atgcttgtat ttcatatcgt
     5161 taccattttt atctattaca cgagcaatta gttcgtcacg gtaaataaca tattgattaa
     5221 gaacaaaatc tcaatacttt tatttccgtc aagacctaaa acatactcga ctaagaaaaa
     5281 aacactgaat gctaaaacag caaaaaatgc tattatccaa ttacctgatg tcaggtgctc
     5341 gttgttgaaa ggatgaggat attttatgga tcaggccggc attattcgcg accttttaat
     5401 ctggctggaa ggtcatctgg atcatcccct gtcgctcgac aatgtagcgg cgaaagcagg
     5461 ttattccaag tggcacttac agagaatgtt taaagatgtc actggccatg ctattggcgc
     5521 gtatattcgt gctcgtcgtt tgtcgaaatc ggcggtcgca ctacgcctga ctgcgcgtcc
     5581 gattctggac atcgcgctgc aataccgctt cgactctcaa cagacattta cccgcgcttt
     5641 caagaagcag tttgcccaga ctcctgcact ttaccgccgt tctcctgaat ggagcgcctt
     5701 tggtattcgc ccgccgctac gtctgggtga attcactatg ccagagcaca aatttgtcac
     5761 tctggaagat acgccgctga ttggtgttac ccagagctac tcctgttcgc tggagcaaat
     5821 ctctgatttc cgccatgaaa tgcgctacca gttctggcac gattttctcg gcaactcgcc
     5881 aaccattcca ccagtgcttt acggtttgaa cgaaacacgt ccgagccagg ataaagacga
     5941 cgaacaagag gtgttctaca ccaccgcgtt agctcaggat caggcagatg gctatgtact
     6001 gactgggcat ccggtgatgt tgcagggcgg cgaatatgtg atgtttacct atgaaggtct
     6061 gggaaccggc gtgcaggagt ttatcctgac ggtatacgga acgtgcatgc caatgcttaa
     6121 cttgacgcgc cgtaaaggtc aggatattga gcgatactac ccggcagaag atgccaaagc
     6181 gggagatcgc ccaattaatc tgcgctgtga actgctgatt ccgatccgtc gttaacgctg
     6241 cagctcatct aatgcagggg cgtccagatg cgagatgtcc cctgcggttt caaccaccca
     6301 gccagacgcc agccacaagc tttcctgata atccacgcgc gaaatagaac agttacgcag
     6361 acgtaagcgg cgctctgccc atgctggtaa tccgagaatc gtactcacca ggcatcccag
     6421 tgcaatacca tggctcacca gtagcggtcg gcttccctgc ggtaagtccc ggcaagattc
     6481 cagtgccgca ttaacgcgat cgctgagctc ctgcattgac tcaccttcag gaatacgccc
     6541 gtcaacggtg ccattgacca gttgccgacg ccagttctct tcttcttcgg tgagtgaatc
     6601 gatatttctt gtttccagta cacccatatt taattcacgc aggcgagaat caaagatgat
     6661 gtcacaaccg caggcctggg cgatgatttc cgccgtgcgc cgggtgcgtc ctaaatcgct
     6721 actgatgata tgcgtaatgc caagctcttt ggcacgggtt gccacctgca tcgcctgttg
     6781 ctcacctttg gcggtcagcg ggctgtcaga ctggccctga atacgtcgct cggcgttcca
     6841 ctgcgtttca ccgtggcgga ctaggtatac ctgtaacatg ctttttttcc gtatactgct
     6901 gtaacgttaa ttttctgagt aatgctgatt atgcaccaag ttgtctgtgc gaccaccaat
     6961 cccgctaaaa ttcaggccat tctgcaggca tttcacgaga tcttcggcga aggatcctgc
     7021 catattgcat ccgttgccgt cgagagcggt gtaccggaac agccctttgg cagtgaggaa
     7081 acgcgcgctg gcgcacgaaa tagggtagcc aatgcccgcc gtttacttcc tgaggctgat
     7141 ttttgggtgg cgattgaagc tggcatcgat ggtgacagca ctttcagttg ggcggtgata
     7201 gaaaacgcca gccagcgcgg cgaggcgcgt tcggctactt tgccgttgcc tgcggtgatt
     7261 ctggaaaaag tgcgtgaagg tgaggcgctt ggtcccgtga tgtcgcgtta taccggtatt
     7321 gatgagattg gtcgtaagga aggggcgatc ggcgtgttta ccgccgggaa actcactcgc
     7381 accagcgtct atcaccaggc ggtgatcctg gcgctgagtc cgtttcataa tgccgtgtat
     7441 cagccattgc aggcctgatt aggcgttcac gccgcatccg gcattcggtg cacgatgcct
     7501 gatgcgccat gtcctatcag gcctacaaaa tcaatcgctt ttcagcaaca cgtcttccag
     7561 ccactggcgc agctcgacgg gcgcggcttt cagactatta gatccacgcg taatcgtcgc
     7621 gatgcccgcg ccgagttcat tttttaactc acgctggctc atttcgccgc gcaacagctc
     7681 ttcgacaata cgcacgcgag tccccaacgc ttcgcgctca tctggcgtca gcatcaggtt
     7741 taacaacggt aaatggagat cgttttggta ggcattctta agcaggtcga caaaacgtaa
     7801 ccactcctgg tgacgctgtt ctgccatcgc tgctgaatag ggtgattgtt gggccatagt
     7861 atgtcgccat tgttagcggg ggaagcaaaa tgcctccccc ggttgtactc gctaaagagt
     7921 acgatagcat atcataaacg tgtggatcag taacgacgtc cccattccgt ggcgctcatc
     7981 aacgtcggtt tatcccccat gaaatagcgg tagtaagcgt cataagccag cacgtttttc
     8041 acataaccgc gcgtctcgga gaatggaata ctctcgacaa atgccactgc gtcgatacgc
     8101 cctgcgctgt tgccaagcca ggttcgcacc cgccctggtc cggcgttata agctgctgag
     8161 gagaaaatac ggttattgcc aaactgctga taaacatatt gcaggtaact ggtgccaatg
     8221 ttgatgtttg tttccggatc cagcaattgc ccagggctgc tataaccggg aatagagaac
     8281 atcttcaccg tatgggtcgc ggtaccaggc ataatctgca tcaggccgct ggcccctacc
     8341 ggtgatttca ctttcggatt ccaggcactc tcctgacggg caatcgccat cgcatagctt
     8401 tgcgggatct ccttaccact ggtgtagcgt ttgaaaagat cgttgtaagc cagcgggaat
     8461 cgctcttcca gatgatccca cagcttcccg gcgatcgttg cctgaacgct aagatcccac
     8521 cattggttat tgaaagcata ccgagccagt tgagcctgct ctgtttttga cttgctcttc
     8581 accagattgg cccactcgct acgcgcggtg ttatcgagat tccagtacat caactcgcgc
     8641 acgcgcgcca tctccggccc ctgagtcagg gcgctgtcaa cattctgcgg cgctttatca
     8701 atcttcagct catactcttc gccgatgcgt tgtgcagcaa ccatcgggta gaaaccacgc
     8761 tgttgcatga gttgatgcaa aatctctttt gcttcagctt cacgtccgcg ttccagcaat
     8821 aaatccgcct gccagtaacg ccattcatct ttctctttcg cttccatagg cagacgcgcc
     8881 agccaggtat tcaggccgcg acgatcgccg gtgccaagcg ccattcgcac acggcgttca
     8941 ataagcgaag tagattgcga gcgcataatc gcgtcatcgc gccatttcgc ctgctcgtcg
     9001 gtgacatcgt tgcccatcaa acgccaggcg acaatatcgc gcagctcctg aatctgatct
     9061 tcattaagct gctgtgcctg agcaagcgac gggatcatca gccgtgcatt ttcagcatcc
     9121 tgccgcgcca cactggcaaa cgccaccgcc gccatttgac gggtaaaatc ggtcgcgccg
     9181 gttgtacgcg cgaaggtcag taccgtatta gggttgttcg ccagtgaaat gattgccgaa
     9241 gcgatagtct ggtaatcggc aggcatctgc cctgccagca ctgttaccag gccggtgtta
     9301 cccgctttca tcgccagacg gatacgctct aaatacgcca gcggatcttg tttacctgac
     9361 gcacgccaga cgctaaataa cttgtcacag gcgttaggct ggctcttgcc ggttagccac
     9421 agctctttcg ccccttgcca ggcttcttca ctctgcccgg tgttccattt cgcatagtag
     9481 taattacatt gcgcttcggt agttccgggc ttttccgggc taaaggctaa caagccacgc
     9541 cagtcttcac gccgcgccag ttcattgacg aaacgagatt gcagcgtgcg agcgggagga
     9601 agcgtggggt tagcgcgaac aaagttagtg accgtcaccg ccggttgatt catcaggtca
     9661 tcagtgatct ggcggtattc caggtaggga taaagtgggt aatctttcag tccaggcatc
     9721 atttgttcga ccacatccat ttgtcgatta tcccaggcct gcttgatttg cgcgtaacga
     9781 ctacgctgct catccagtga gtcggctcgc gccacgctgc tgaccgtcag cagacagaca
     9841 ccgacagcca acagccgcca ggtaacgtat ttggcttttt ccacaagcgc atcctctaag
     9901 tgtcaataca tcaatgcagc gtcatgccgc gtcatgccgc gtaatagatt tatgctaacc
     9961 agtcattgcc gtttacgcca cgttacggac acttttttac ttttacgggg tgatcgactg
    10021 gctgggatta gacagggaaa caggctacac ttcgcctttg aaaactcatc atcacgataa
    10081 acatagaggc gaagtccaac gtggctcaat tcgtttatac catgcatcgt gtcggcaaag
    10141 ttgttccgcc gaaacgtcat attttgaaaa acatctctct gagtttcttc cctggggcaa
    10201 aaattggtgt cctgggtctg aacggcgcgg gtaagtccac cctgctgcgc attatggcgg
    10261 gcattgataa agacatcgaa ggtgaagcgc gtccgcagcc agacatcaag attggttatc
    10321 tgccgcagga accgcagctg aacccggaac acaccgttcg tgagtccatt gaagaagcgg
    10381 tttctgaagt ggttaacgcc ctgaaacgcc tggatgaagt gtatgcgctg tatgccgatc
    10441 cggatgccga ttttgacaag ctggccgctg aacaaggccg tctggaagag atcattcagg
    10501 ctcacgacgg tcacaacctg aacgttcagc tggagcgtgc ggcggatgcg ctgcgtctgc
    10561 cggactggga cgcgaaaatc gctaaccttt ccggtggtga gcgtcgtcgc gtggcgttgt
    10621 gccgcctgct gctggaaaaa ccagacatgc tgctgctcga cgaaccgacc aaccacctgg
    10681 atgcggaatc cgtggcgtgg ctggaacgct tcctgcacga cttcgaaggc accgtggtgg
    10741 cgattaccca cgaccgttac ttcctcgata acgttgcggg ctggatcctc gaacttgacc
    10801 gcggtgaagg tattccgtgg gaaggtaact actcctcctg gctggagcag aaagatcagc
    10861 gtctggcgca ggaagcttca caagaagcgg cgcgtcgtaa gtcgatcgag aaagagctgg
    10921 agtgggtacg tcagggaact aaaggccgtc agtcaaaagg taaagcacgt ctggcgcgct
    10981 ttgaagaact caacagcacc gaatatcaga aacgtaacga aaccaacgaa ctgtttattc
    11041 cacctggacc gcgtctgggc gataaagtgc tggaagtcag caacctgcgt aaatcctatg
    11101 gtgatcgtct gctgattgac tccctgagct tctcgatccc gaaaggagcg atcgtcggga
    11161 tcatcggtcc gaacggtgcg ggtaaatcga cgctgttccg tatgatctct ggtcaggaac
    11221 agccggacag cggcaccatc actttgggtg aaacggtgaa actggcgtcg gttgatcagt
    11281 tccgtgactc aatggataac agcaaaaccg tttgggaaga agtttccggc gggctggata
    11341 tcatgaagat cggcaacacc gagatgccaa gccgcgccta cgttggccgc tttaacttta
    11401 aaggggttga tcagggtaaa cgcgttggcg aactctccgg cggtgagcgc ggtcgtctgc
    11461 atctggcgaa gctgctgcag gttggcggca acatgctgct gctcgacgaa ccaaccaacg
    11521 acctggatat cgaaaccctg cgcgcactgg aaaacgccct gctggagttc ccgggctgcg
    11581 cgatggttat ctcgcacgac cgttggttcc tcgaccgtat cgccacccac attctggatt
    11641 accaggatga aggtaaagtt gagttcttcg aaggtaactt taccgagtac gaagagtaca
    11701 agaaacgcac gctgggcgca gacgcgctgg agccgaagcg tatcaagtac aagcgtattg
    11761 cgaagtaatg cgtaaaatgc cggatgcggc gcaccagcat cgcatccggt attagttaaa
    11821 tgattaatgg gaagcaagtt cccggaaaaa ggcaggattt tctttaattg tggcaagtac
    11881 ttttgcagcc aatcccgtag gatttcttcg cttcgattcc cagcttttaa tggtatccac
    11941 actggttccc agagcttttg ccatctcact ttgagaaacg ttgagttgct cacgaatagc
    12001 tttaacatcc gcaatttcgt agcgggtcac ccgtgcaggt tcttttaaac cttgtttaat
    12061 ctcgacagcc tcttccagag aggttttcaa ctcatcaaaa aaactcattt tatatctcgc
    12121 ctttgagcag tttcgtcaac ttctttagtt gagctttttc cgtatctgtc agactatctt
    12181 tggcattttt cggataggcc ataatcaaat aaataatctc ttccgtcgcc agaaagtaaa
    12241 ttattctgac actgccactt tttccctgtg aacccgcagc cattcggact ttacgcaatc
    12301 caccagtctg ctgaatgaga tcacctttat cgggagttcc gatgagttct ttttgtaatt
    12361 cctttaattc atcatcggtt gcaatctgct tgatctgacg cgtaaacatt ggcgtctcta
    12421 taaatattat cgccttaccc attccccctc cctggcaggt acattgtaca cctaaatgga
    12481 gtactaagta cacttttaaa tcagtaatcc cactcaacat cttataaaga tgattatctt
    12541 tgctccccca tcatctcccg caccagctcc acgcagcgca ggaaacggct gtcgtaatcg
    12601 tcctcttcaa cccgcacgaa ttcgatattg ttctcttcca gcatctccac cagcaagttc
    12661 tggaactctt tgcgatccac cgaactgccg aggctgcgta aaccatccgc cacccacggc
    12721 gtgttgttct ccagcaggat caccagatcg aaacggtatt catcaatcaa cgcctgcacg
    12781 aacggatgct cacgcccttc gtactttttg cagaacgcct gggtagtgac aaaatcggtg
    12841 tcgataaacg ccactttatt ggcatatttc actgcaaaat caatatattg cgcatgtccc
    12901 agagcgattt tatcgtagtc ggaatactgc aacgcgatct catcgccacc aaggtgtgaa
    12961 aagacataat cacgaccata ttcccacgca ctggtagtgt tgaagatatt ggcaagtttg
    13021 tttaccaggg tggatttacc gctcgattca ccaccgagga tcgccacggt acgcacaaag
    13081 aatggcttca cttcagtggg aatatattcc cagtagcgga acgggttttc gcggatctgc
    13141 gcaccgctga tactcataaa ggtacgcttc ggatcgacca gcaccgtctc gatccccaga
    13201 tgttccatat actgcggcgc atcggcttct tccgaggtgt agatcaggtc cggctgaatg
    13261 cctttttcgg ccataaactt tttgatgccg ttgctccaca catcccagcc atgtggatac
    13321 ggttccatgc cctcttcgtt aaaagcatga atgcggatat ttttttgata cttaaaggtc
    13381 tgcaataacc agcgcagacg atccggcacg gttggctgct gcgacatggc gctgtcttcg
    13441 aacaatgcgc ggtcgcgggt atcgtcaaaa cccataatga tatgcagctc gtcaacctgg
    13501 ctacaggcgc gctggataag gtagatatgt ccagtatgca gcgggtaaaa cttaccgaat
    13561 acgacaccga tcgttttctt ctgccgggga aattcaagtc ccaaataacg gtgcaatgcc
    13621 tccagctttt gtgcgctggg gcttttaatt ttggcattca gtaactggct taaataccct
    13681 ttggtcatac cgctggcatc agctacctgc tgtagcgtgc agccctgttg cttgatggca
    13741 gttttcaggt aatcaaatga cgacatatct ccctccgtat ctctcattat aagtcgtcga
    13801 acacgctaag cgcgtcggag agttttttaa cgccaaaaat ctgcatccct tccggcgctt
    13861 ttttcggcac gttagctgcc ggaacaattg cccggcgaaa accgtgtttc gccgcttctg
    13921 agattcgttc ctggccgcta ggcaccgggc ggatctcccc tgccagcccg acttcaccaa
    13981 acaccaccag atcctgcggt agcggtctgt cacgcaggct ggaaaccatc gccagcagta
    14041 acgctaaatc ggcactggtt tccgtcacct tcacgccacc gaccacgtta acaaacacat
    14101 cctgatcggc catttgtaga ccaccgtgac ggtgaagcac cgccagcagg attgccagac
    14161 ggttttgctc cagcccgact gccacgcggc gtggattcgc catcatcgag tgatcgacga
    14221 gcgcctgaat ctccaccagc agcggacgcg ttccttccca cactaccatc actgagctac
    14281 cggaggtcac ttcatcgccg cgacttaaga aaattgccga agggttgctg acttcacgca
    14341 gcccctgctc ggtcattgcg aagacaccca gctcattcac cgcgccgaag cggtttttat
    14401 ggctgcgtaa ggtgcgaaaa cgtgagtcgg catcaccatc caaaagcacc gaacagtcga
    14461 tacagtgttc cagcacttta gggccagcca gcgagccatc tttggttacg tgcccgacca
    14521 tgacaatcgc cacaccgcgc gttttggcga agcgcgtcag ataagccgcc gtttcacgca
    14581 cctgtgccac gctgccaggc gatgattgta catccgccat atgcatcacc tggatggagt
    14641 caattaccat cagcttcggt tgctcttctt cggcaatcag gcagatctgc tcgatgctgg
    14701 tttccgacag catattaaga ttgtcagtcg gcaggccaag gcgatgagcg cgcattgcca
    14761 cctgttgcag cgactcttcg ccagtgacat acagcgtttt catctgctgc gccagtttgc
    14821 acagcgtttg cagcaacagc gtggatttcc ccgcaccagg gttaccgcca atcagaatgg
    14881 cgcttcccgg caccacgccg ccgcctaata cgcggtcgaa ctctttaaat ccggtggaaa
    14941 aacgcggcag ctcttcaagg ctgatgtcgg aaagtttctg gacttttgcc actccggcgc
    15001 taccggcata gccgctgaga cgctcgttac gcgccaccgt tggcgacgct gcaagacgca
    15061 cctcggtgat ggtgttccag gcatgacagg cactgcactg cccctgccag cgcggataat
    15121 cggccccgca ttcattacaa acaaaggcgc gttttggagc ttttgccacg atgtacctcg
    15181 ttaatgctgt gccgcccgca ggatgacggg cggacaatta cttctgattc aggctgccag
    15241 agaggatgca gaataccccc atcaggtcag cgtgacggat ggtgacttcc gtcttttcat
    15301 tcacttttgg cttggcatgg taggcaatcc ccagccctgc cgctttgatc atcggcaggt
    15361 cattggctcc atcgccaatc gccacggtct gcgccagcgg gatttcatac tcctgcgcga
    15421 ggcgggtcag ggttttcgct ttatattgcg cgtcaacgat gtcgccgatc acattgccgg
    15481 taaatttacc gtccatgatc tccagttcat tggctaccac ggctgtcagg cgcagcttgt
    15541 cgcgcaggta ttcagcaaag aaagtaaagc cgccggaggc aatcgccact ttccagccca
    15601 gcgtttccag cttgagtacc agctgcgtta agcctggcat cagcggcaga ttttcacgca
    15661 cctgttgcag aatattggcg tcagcgcctt tcagcgtcgc cacgcggctg cgcaggctgg
    15721 cggtaaaatc gagttcgccg cgcatcgccc gttcggttac ctccgccacc ctctcgcccg
    15781 ttccggccag tttggcaatt tcatcaatac attcaatctg gatggcggtg gaatccatgt
    15841 ccatcaccag caaccccggc gtgcgcaggt gtgggatttt ccccagcggc gcgacatcca
    15901 gttgcgcttc atgggccagg cgcgtagccc gcgcagtgag tgaacctgcc agacgaatca
    15961 cctgataatc ttccacgcac caggcggcaa caatcaccat agccgcgccc agtttgctct
    16021 ggtattgggt cagacgttgt ttatccagcc cacgaccata cagcagccag ccgctacgac
    16081 ctgcgtggta atccagtggc atcacttcat caccacttaa tgaaagaggc agaccaggcc
    16141 ataaagagac atcttcaggc aggtcgcacc aggtaatgtt aggcattaag gctcctgtaa
    16201 aatcgttcga agcagggaaa ataacgcatg aggctacctt gtatccattg cttctggcaa
    16261 cattaagtct caaattttca aagggtggaa gatggctcgc acaaaactga aattccggct
    16321 gcatcgggca gtgattgtcc tgttctgtct tgccttgtta gtggcgctga tgcagggggc
    16381 gtcatggttt agtcaaaacc accagcgaca gcgtaatccg caactggaag aactggcccg
    16441 caccctggcg cggcaggtga cgctaaacgt tgcaccgctg atgcgtaccg actcaccgga
    16501 tgaaaaacgc attcaggcga tcctcgatca gttaacggat gaaagccgca ttctcgacgc
    16561 aggcgtatat gatgagcaag gcgatcttat cgcccgttct ggcgaaagcg tcgacgtgcg
    16621 cgaccggctg gcgctcgacg gcaaaaaagc gggcggctat tttaaccagc agattgtcga
    16681 acccattgcg ggtaaaaacg gaccgctcgg ctatctgcgc ctgacgctcg acacccatac
    16741 gctcgccacc gaagcccaac aggtggataa caccactaac attttacgcc tgatgttgct
    16801 gctctcgctg gcaatcggcg tagtgctgac ccgcacgctg ctacagggta aacgcacccg
    16861 ctggcagcaa tcgcccttcc tgttaaccgc cagtaaaccg gtgccggaag aggaagaaag
    16921 cgagaaaaaa gagtgaccca ttactacaag aaaggaaatc gttatgtcca cattacgcct
    16981 gctcatctct gactcttacg acccgtggtt taacctggcg gtagaggagt gtatttttcg
    17041 ccagatgccg gctacgcagc gcgttctgtt tctctggcgc aatgccgaca cggtagtgat
    17101 tggtcgcgcg cagaacccgt ggaaagagtg taatacccgg cggatggaag aagataacgt
    17161 ccgcctggcg cggcgcagta gcggtggcgg cgcagtgttc cacgatctcg gcaatacctg
    17221 ttttaccttt atggctggca agccggagta cgataaaacc atctccacgt cgattgtgct
    17281 caatgcgctg aacgcgctcg gcgtcagcgc cgaagcttcc gggcgtaacg atctggtggt
    17341 gaaaaccgcc gaaggcgatc gcaaagtctc aggctcggcc tatcgcgaaa ccaaagatcg
    17401 tggcttccac cacggcactt tgctgctcaa ttccgacctt agccgcctgg caaactatct
    17461 caatccggat aaaaagaaac tggcggcgaa aggcattacc tcagtgcgtt cccgcgtgac
    17521 caatctgacc gaattgctac cggggatcac ccatgagcag gtttgcgagg ccataaccga
    17581 ggcctttttc gcccattatg gcgagcgcgt ggaggcggaa atcatctccc cggacaaaac
    17641 gccagacttg ccaaacttcg ccgaaacctt tgcccgccag agtagctggg aatggaactt
    17701 cggtcaggct ccggcattct cgcatctgct ggatgaacgc tttacctggg gcggcgtgga
    17761 actgcatttc gacgttgaaa aaggccatat cacccgcgcc caggtgttta ccgacagtct
    17821 caaccccgcg ccgctggaag ccctcgccgg gcgactgcaa ggctgtctgt accgcgcgga
    17881 tatgctgcaa caggagtgcg aagcgctgct ggttgacttc ccggaacagg aaaaagagct
    17941 acgggagttg tcagcgtgga tggcaggggc ggtaaggtaa ctcgctgatt aaaatatcca
    18001 gggaaggaaa tatgatcgat ctggaaaacc aggaacggga gattatcaat ttaatgttca
    18061 gccagggtat atcctggcta accgccgtgc gtatcaggca taaactatca cttgctgaag
    18121 taagtaaaat gttaggtatc tccattaatt ctctaaaaca gatcgaaaag acggaacggc
    18181 ttagttcaaa catcaaaagc aaaatggcag gaatttatgg ttgtccacca gagttgctga
    18241 tatgcccttc ctggatgacg gctgaacata aatgaataga ttcgtgaata gatacttgaa
    18301 tggattttat tttctatcca tatgaaaaat aatagtaaat ctataaatca gttgctattg
    18361 atgaataaat actaaaaagc cggagcagtc tcccactccg gcttcacacg acaatcgcct
    18421 ttcagcaaaa cacaattact ctttatcgcc cagcagaacg gattccagcg cgattttgat
    18481 catgtcgttg aaggtggtct gacgctcagc ggcagtggtc tgctcgtgag tgcggatgtg
    18541 gtcagacacg gtgcagatgg tcagggcttt cgcgccaaat tctgcagcga cgccgtagat
    18601 accagccgct tccatttcca cgccgaggat gccgtatttt tccatcacgt cgaacatttc
    18661 gccgtccgga gagtagaaca ggtcagcgga gaacaggtta ccaacgcgag catcaacgcc
    18721 cagtgctttt gccgcgtcta ccgcgttacg caccatgtcg aagtcagcga tagcggcaaa
    18781 gtcatggtct ttaaaacgga tgcggttaac tttggaatcg gtgcaggcac ccataccgat
    18841 aacgacgtcg cgcagtttta cgtgcggcag aacagcgcca caggaaccca cgcggataat
    18901 tttcttcacg ccgaaatcag tgatcagttc tttggtgtag atggagcagg acgggatacc
    18961 cataccgtgg cccattacgg aaattttgcg gcctttgtaa gtaccagtga agcccagcat
    19021 accgcgaacg ttgttcactt cacgggcatc ttcaaggaaa gtttcagcaa tatacttcgc
    19081 acgcagcggg tcacctggca tcaaaactac gtcagcaaaa tcgcccattt ctgcattaat
    19141 gtgtggagta gccatcgttt tatccttttt gtgacatatt aaaggcggag tcactgctcc
    19201 gcccaaatcc atcagaacat ggctttgcca tattccatat cagaagtacc aaaatatttt
    19261 gccagagtct ggccgatatc cgcgaaggtt tcacgatgac ccagcgaacc tggtttcact
    19321 ttcgggccgt aaaccagtac cggaatgtgt tcacgcgtgt ggtcagtacc ggtccaggtc
    19381 gggtcacaac cgtggtcagc agtgagaatc agaatatcgt catcgcgcag caaagacatc
    19441 agctccggca gacggcggtc gaacagttcc agacccgcag cataaccggc gacgtcgcgg
    19501 cggtggcccc aggaagagtc gaagtcaacg aagttggtga agacgatggt gttatcaccc
    19561 gcttctttca tctctttgat ggtggcgtca aacagcgcgt ccagcccggt cgctttcact
    19621 tttttggtga taccgcagtt ggcatagatg tctgcaattt taccgaccga aaccacctgg
    19681 ccgtgttttt catcaaccag tttctgcagc acggtcggtg ctggcggctc aacagccagg
    19741 tcgtgacggt taccagtacg ctggaagtta ccagctttgt cgccgataaa cggacgagcg
    19801 ataacacgac cgatgttgta gccgccgttg gtcagctctt cacgggcgat ttcgcacagc
    19861 tcgtagagtt tatccaggcc aaaggtttct tcatggcagg caatctggaa cacggagtca
    19921 gcggaggtat agaaaatcgg cttgccggtt ttcatgtgct cttcgcccag ttgatccaga
    19981 atgaccgtac cggaagagtg gcagttaccg aggtaacccg gcagattagc gcgttcaacc
    20041 agtttatcca gcagctcttg cgggaagctg ttttcgtgat cggagaagta tccccactca
    20101 aacagaaccg gcacaccagc aatttcccag tgaccagacg gggtatcttt accggatgac
    20161 atttcgtgcg cccatgcgta cgcgccgata acttcagcgt tgccgtccat tcccgccgga
    20221 atgaaaccgg tagaaccttc gtgtgctttc gccagcccca gacgggtcag atttggcaga
    20281 ttgagcgggc ctttacgacc gttatcagct tcgcctttgg cacaagcttc tgcgatatga
    20341 cccagggtgt cagccccgac gtcaccaaag cgttctgcat cttctgtagc gccgatgccg
    20401 aatgagtcca gcaccataat aaatgcacgt ttcatatgtt ctccgtactc agtgcttcaa
    20461 atttaaatga tcagatcagt atactgttat tcgctgatac ggcgatagac agttggtgtg
    20521 ctttccggtg ctttatcggc aagtttaatt gccgctttca ccgatttcgc cgcttcctgc
    20581 cagctgtttt cgtctttcgc gtggataact gccagcggac gctgaccgtc tacctggtcg
    20641 cccagacgcg ccatatcagt aaagccgacg ctgtaatcga tggtgtcaga tgcctgacgg
    20701 cgtccgccgc ccattgcaac cactgccatc cccagtgcgc gggtatccat ttcactgaca
    20761 aacccttcgg tatcagcata gactgctttc gtcagcatcg ctgtcggcag atacttcgcg
    20821 tagttctcaa cgaagtcggt cgggcctttt tgtgccgcaa ccatacgacc aaagacttct
    20881 gccgctttac cgttgtccag caccgcctgc aatttcgcgc gcgcttcggc gtcatctttc
    20941 gccagtttgc cggagatcag catctccacg cacagtgcca tcgtgacatc aaacagacgc
    21001 gggttacgat attcacccgt caggaactgc accgcttcac gaacttcaac cgcgttacct
    21061 gcactggagg ccagtacctg attcatatcg gtgagcagcg cggtggtgcg cacgccagcg
    21121 ccgttagcca cgccaacaat cgcttcggca agggcttcag agagttcgta ggtcggcata
    21181 aacgcgccgc tacccacttt cacgtccatc accagcgcgt ccagaccttc cgccagtttc
    21241 ttcgccagaa tagaggcggt gatcagcggg attgaatcca cggttgcggt aatatcgcgg
    21301 gtcgcgtaga aacgtttatc cgccggagcc agtgagctgg tctggccgat aatcgccacg
    21361 ccgacgtctt taataatttc gcggaaacgg ttgtcatccg ggaaaatgtc gaagccaggg
    21421 atggattcca gtttgtcgag cgtaccgcca gtatgaccga ggccgcgacc ggagatcatc
    21481 ggaatatagc cgccacaggc agcgaccatc ggccccaaca tcagcgaagt cacatcgccg
    21541 acaccgccgg tcgagtgttt atcaacaatc gggccattca gttgcaggct tttccagtcg
    21601 agaacggttc ctgaatctcg catcgccatg gtcagcgaga cacgctcagg cattgtcata
    21661 tcgtggaaga aaatggtcat cgcgagggcg gcaatctgcc cttcggagat agtgttgtcg
    21721 cgaatgccgt tgataaagaa acgaatttct tcatcactca gcgcatgacc atcacgtttt
    21781 ttacgaataa tttcttgtgc gagaaacaag gtaacctccc gtggaaaaga gacgaggtaa
    21841 tcggggaacc ggatgagcat cgctgcatca tccggcaaat caagccttag tagctgctgg
    21901 cgctcttacc gtcgccgtgg cccaaagctt tcagcaggct tgccagcaag ctggaagcac
    21961 caaagcggta gtgacgcgca tcagcccagt cagcaccgaa cagttcatct gcaatggcga
    22021 gatatttctg cgcatcttcc gcagtacgca cgccgcccgc cggtttgaaa ccaacggttt
    22081 tttctacgcc catatcgcgg atcacttcca tcatgatgcg cgcgctttcc ggcgtcgcat
    22141 tcacagccac tttaccggta gaggttttga tgaagtcagc acccgctttg atggagattt
    22201 cagacgcttt acggatcagc gcttcgtctt tcagttcgcc ggtttcaata atcactttca
    22261 gcagtacgtt cgccgcagcg caagcctctt tacaggcttt caccaggtca aaaccaacct
    22321 gctcgttacc cgccatcagc gcgcggtacg ggaacaccac gtcaacttca tcggcaccgt
    22381 aggcgattgc cgcacgggtt tctgccagcg caatgtcgat gtcgtcgtta ccgtgtggga
    22441 agttagttac cgtagcgata cggatttccg gggtgccctg ctctttcaga gttttgcgag
    22501 caatcgggat aaagcgagga tagatacaga tagcggcggt attgccgacc ggagttttgg
    22561 cctgatgaca cagggcgatc actttctcat cggtgtcgtc gtcattcagg gtggtcaggt
    22621 ccatcaattt cagtgcacgc aggctgcttg ctttcagatc agtcatttca ttctcctggc
    22681 ttgtcgccaa taaaattcac cttgcgagtt tgttagtatt ctaacatcta ctccgcaaca
    22741 cacttcgata tacatcacaa ttaaggaaat ctacttacaa gtttgcatca ctgtaatgcg
    22801 atctggttca aataattcac tttcaaatga atgcgtcagt ggtggcaaac gcatcaggat
    22861 caaagtgaac atcacgaaac ttcttacaat ggcgcatcac cttcggcata aaaggaaaag
    22921 aagatgccca cttctcatga aaatgcactg caacaacgtt gccagcaaat tgtcaccagc
    22981 ccggtactta gcccggagca gaagcgccat tttctggcac tggaagcaga aaacaatctg
    23041 ccttacccac agctgcctgc cgaagcccgc cgcgcgctgg atgagggggt aatctgcgat
    23101 atgtttgaag gtcatgcgcc gtacaaaccg cgctatgtct tacccgatta cgcccgtttt
    23161 ttggccaacg gttccgaatg gctggagctg gaaggcgcaa aagatcttga tgacgcactc
    23221 tctctgctga ccattcttta ccaccacgtt ccttcggtca catcgatgcc ggtctacctg
    23281 gggcaactgg atgcgttgtt gcaaccgtat gttagaattc taacacaaga cgagatcgat
    23341 attcgaataa aacgtttctg gcgttacctc gacagaaccc tgccagacgc ctttatgcat
    23401 gccaatatcg gcccgtctga ttcgcccatt acccgtgcga tattacgtgc agacgcagag
    23461 ctgaagcagg tttcaccgaa cctgaccttt atctacgatc ctgatatcac ccctgatgac
    23521 ctgctgctgg aagtggcgaa aaacatctgt gaatgtagca aaccgcacat cgccaacggt
    23581 ccggtgcatg ataaaatttt cacaaaaggg ggctacggga ttgtgagctg ttacaactca
    23641 ctgccgctgg cgggtggtgg cagcacgctg gtacgcctta acctgaaagc cattgccgag
    23701 cgcagcgaat cgctggatga cttctttacg cgcactctac cgcactactg ccaacagcag
    23761 atcgccatca tcgatgcgcg gtgtgaattc ctctatcaac aatcacactt ctttgagaat
    23821 agcttcctgg tgaaagaagg gctgattaac cctgaacgtt ttgtgccgat gtttggcatg
    23881 tatgggctgg cggaagcggt taacttgctg tgtgaaaaag aagggattgc cgcacgctac
    23941 ggtaaagagg ccgccgcaaa tgaagtgggt tatcgcatta gcgcgcaact ggcggagttt
    24001 gtcgccaata cccccgtgaa atatggctgg caaaaacgcg ccatgttaca cgcacagtcg
    24061 gggataagtt ccgatatcgg caccacgccg ggcgcgcgtt tgccgtatgg tgatgagcca
    24121 gatccgatta cccatctgca aactgtcgcc ccgcatcatg cttattatta ttccggcatc
    24181 agcgacattc tgacgctcga cgaaaccatc aaacgtaatc cgcaggcgct ggtacagctt
    24241 tgcctcggtg cctttaaagc cggaatgcgt gaatttaccg ccaatgtcag cggtaacgat
    24301 ctggttcgcg ttaccggtta tatggtgcgt ttgtcggatt tagaaaaata tcgcgccgaa
    24361 ggttcacgca ccaacactac ctggctggga gaagaagccg cacgcaacac tcgtattctg
    24421 gaacgccagc cgcgcgtgat aagccatgaa cagcagatgc gctttagtca gtaagattat
    24481 ccccttctct tgcgttgacg ggccaggcag tcgtctggct ctgtttttgc agggctgcaa
    24541 tctgcgctgc aaaaactgtc acaatccgtg gacgatgggg cgctgtaatg attgcggaga
    24601 gtgcgtgccg cagtgtccgc atcaggcgtt gcagattgtc gacggcaaag tggcgtggag
    24661 cgcggctgtc tgcgagcaat gtgatacctg cctgaagatg tgtccgcaac atgctacgcc
    24721 aatggcgcaa tccatgagcg tggacgaggt gcttaaccat gtccgcaaag cggttctgtt
    24781 tattgaaggg ataacggtaa gcggcggcga agccacgacc cagttgccgt ttgtggtggc
    24841 gctgtttact gccatcaaaa acgatccgca actgtgccat ctcacctgcc tcatggacag
    24901 taacggcatg ttgagcgaaa ccggctggga aaaattgctc ccggtgtgtg acggcgcaat
    24961 gctcgatctc aaagcgtggg ggagcgaatg tcatcagcaa ttaaccggac gcgataatca
    25021 gcagatcaag cgcagcatct gtttgctggc agagcgcggt aagctggcgg aactgcgttt
    25081 gctggtaatc cctggccagg tggattattt gcaacacatc gaagaactgg cggcgttaat
    25141 caagggactt ggcgatgttc cggtacgcct gaacgcgttt catgcccacg gcgtgtatgg
    25201 cgaggcgcaa agctggccga gtgccacgcc ggaggatgtg gaacaactgg ctgatgcgtt
    25261 atgggagcgc ggggtgagcc ggttaatatt tccggcgcta tatttgtgac atgccggata
    25321 cggcctacgg atcggcacag acctgtaggc atgataagac gcgtcaagcg tcgcatcagg
    25381 cattgtgcgc cagctgccgg atgcggcgtg aacgccttat ccggcctacg gttcggcaca
    25441 gacttgtagg catgataaga cgcgtcagcg tcgcatcagg cattgtgcgc caacggccgg
    25501 atgcggcgta aacgccttgt ccggcctacg gcacgttaaa caacgtatag gtgttattaa
    25561 gcaacacttc ggcaatctca tccgccggtt ccggtcgtaa ctcgcaaagc acggcaaaca
    25621 cacgggcggc ctgttcaggg cgattaggct gcccctgaaa accattgagc ggcatatctg
    25681 gtgcgtcggt ttccagcaac aacgatgcca gcggtaattt tgcgatgaca tcgcgggttt
    25741 tactggcgcg tggataggtg atagtaccgc ctacgccgat tttgtagccc agctgtacaa
    25801 accgttcggc ctgttgcagg ctgccggaaa aaccgtgaac cacgccagag cgcggcaaat
    25861 cgtggcgttt aagatgcatc gccagtttgt cgtgcgtgcg tcgtgaatgc aggatcaccg
    25921 gcagatcgta gcgtttcgcc agtttcagtt gttcgtcgag taaccactgc tgcctctcaa
    25981 attgcggatc gtcgccgaag agatccagac cgatctcccc caccgccacc atcttagttg
    26041 gacgtctttc cagcgcctgc tgtagctgat caagagacac atcgctatgt ttttccaaca
    26101 taccaggatg caagcccaat gcggcataca gcggttgata tttttccgct aatgccaaca
    26161 cacgggcaaa attttccgcc tctgtcgccg gaataatgat cttgcctacg cccgcttgtg
    26221 ccgcgcgttg caggctggcc tcttcatcgc cactaaacgg cgggaaatca aaatggcagt
    26281 gggtatcgat aaaacggcaa atcaagccag atcctcattg ttaaatgtcg ggtcgttcgc
    26341 ctgcggagca tcgctcactt cggcaaccag cgtatcgttg gcgactggcg caggcggaat
    26401 gacaatcgat tccggcgtca ccactggcac cagatggcgg gtaagcggcg ctttttcagt
    26461 caataattta ccgaccgtgg cgaggaaata acgaccgcat agacgcccaa gtttatagtc
    26521 ttcacgcagc gccggaatcc gactgccaag cgcgatacta tgcaatggct tcggcggata
    26581 aatttcgaat atccgcagtt tgcccggtgg tttctcaata aattgctgaa tgtcacgata
    26641 gctggtttca tgatgctgca ccagattgac cagcggctgc aggctactgt cacccagcca
    26701 gcgttccatg cgtttgaacc actgcggcgt gtagtacatt tgcgacggca cggtgcggat
    26761 gacgaccaac gtttttgctc cctgccttgc cgcctcttta accggaatcg catcactgat
    26821 cccgccatcc aggtagttaa tgccttccag cgacactccg ctacgataaa agccaggtat
    26881 cgcactggag gcgcgaatca catccagcca gttttgtttg gttggtaaaa agtaattcgg
    26941 cgcatagtca tcctgacgac aggcgcacat ataaaacgat ttgccgctgt caaacaaccg
    27001 cgcggcggta tccatttgca acggcatctg gcttgcggtg gcctccacca gccagtcgag
    27061 atcgataaga tttcctccac ggacaaagcg caatggatcg aaaaattcgc gttttgtggt
    27121 gtaacgcatg atgactttgc gcgcgtaacc gggctgattg cagatatacg ccgagaggtt
    27181 ctgcgccccg gcagatgtgc cgagataaag atcgaaagga ttaaactgcg cgcgcataaa
    27241 ctcatccagc acgccagccg tgaaaattcc acgttgtccg ccgccttcgc acaccagagc
    27301 aatccgtcca ggctggaacg gccttagcga caacggcgca atattaccaa gcgttacagg
    27361 tattcgctgc cccacctctg ccttcctgtt tttgttgtga aagaatgaca aagtaacgca
    27421 atttattatt cgctaaaacc gcaaaagcca gtccgctgga ctggctttgg ataaaatttt
    27481 cttaatggtc tgaaatctag ggtcgttttc ggcccatgaa caaactcacc aggaacagaa
    27541 taatcccgac gacaaagaca attttagctg cgcctgcagc agtaccggcc agaccaccaa
    27601 acccaagtgc ggcggcgatt aacgcgataa ccagaaatat gatgccccaa cgaaacataa
    27661 gcgtctcctt taccatagtt aatgtcaccg cttgatatga gcgtgaaaat cactcaatgc
    27721 gatattttta gtgtggtgca catcgcgcct cccgacaaag ttcgggaggt cgaattacga
    27781 cgaattactt agttttcaga tcatttttaa cgcttttcac accatctacc gctttggcga
    27841 tactttcagc acggtcgctc tgtgcctgag aatctacggt accggagagc tggaccacgc
    27901 catcggtggt ttcaactttc acatgacggg aagggacaat gtcatctgcc aacagtttgg
    27961 ctttgatttc actggtggtg gcggtgtcac ccgcgtagcc cttcacagag ccttctttag
    28021 cgtcgcgaac gtgcagtttg tcgctgacag aggtcacgcc ctcaacacct ttcgccactt
    28081 tcaccgcctc ttcggcctga gcctggcttt caacgaaacc gctcagggtc acgacttttt
    28141 gatcggtttt gacagagata tcggtgctct tgatgttgtc atgatccacc agggccgcct
    28201 tcactttcgc ggtgatggcg ctgtcatcca tgaaattacc gactttattc atagagctat
    28261 cgactttttg ccctgcgctt tcattggtag tctgcgcgtt gttttccgcg taggcagagc
    28321 cggtcgcgac ggcagaggtc aacattacag ccagcagagt tttcgaaatc ttcagtcttg
    28381 tcatagtcat cgatttattc ctgtatgttt gctcgtaatt tgagcctggc aacacgaggt
    28441 tgcattgctg aatagggaga gacttcaccc tctacagaag tcaatggccg ccatcacaaa
    28501 agcgatgggt gatgaataac gaccattaca gcctctgaat cagttattaa tatcggtaga
    28561 atgacaatca acggctttag atactgatat ctacgcattg aacggtattt aacgccgtca
    28621 gaaatgtcat tactttgtta aatatagatc acaattttga aaccgctcgg gatatcagcg
    28681 aaaacataag caaaagtgaa tgttttaaga acattccgta agcggctaat aaggaaggga
    28741 aattgacagg gcgcagcggc taccgcgccc tggaggcaag aattaatgct cgcgggtctg
    28801 gtggaactga acgtctggat aacgttcctg cgccaggcgc aggttgacca tgctggtagc
    28861 gatgtaagcg aggttatcgc cgccatcaag cgccagttgg ctttcgttct tacgcttgaa
    28921 ctcttcgaat ttcttcgcgt ccgcacattc tacccagcgg gcagtggcaa cgttgactga
    28981 ctcatacact gcttcaacgt tgtattcgct cttcagacgc gctaccacca catcaaactg
    29041 cagcacacca accgcaccaa cgatcagatc gttgttggag atcggacgga acacctgcac
    29101 cgcgccctct tcggaaagct gtaccagccc tttaagcagc tgtttttgct tcagcggatc
    29161 tttcaggcgg atacgacgga acagttctgg cgcgaagttc ggaataccgg tgaacttcat
    29221 catctcaccc tgggtaaagg tgtcgccgat ctgaatggtg ccgtggttgt gcagaccaag
    29281 gatatcgcca ggatacgctt cttcaacgtg cgaacggtca cccgccataa aggtcagcgc
    29341 gtcggagatc accacatctt tcgcagtacg cacctggcgc agcttcatgc ctttttcata
    29401 tttaccggac accacacgca taaacgccac gcggtcgcgg tgtttcgggt ccatgttggc
    29461 ctgaatttta aatacgaagc cggtaaattt gtcttcgctc gcctctacgg tacgggtatc
    29521 agtctgacgc ggcatcggcg caggagccca ctccaccagg ccatccagca tatgatcaac
    29581 gccgaagtta cccagcgcag taccgaagaa taccggagtg atttcgcccg caaggaacag
    29641 ctctttgtcg aactcgttag acgcgccttt caccagttcc agttcgtcac gtagctgctg
    29701 tgccagatct tcaccaaccg cagcgtcgag atccgggtta ttcagccctt taacaatacg
    29761 gacttcctga atcgtgtggc ctttaccgct ctgatagaga taggtttcat ctttataaag
    29821 gtggtaaacg cctttaaaca gcttgccgca gccaatcggc caggtgatcg gtgcgcagcc
    29881 gattttcagc tcgttctcaa cttcatcgag cagctccatc gggtcgcgga tatcacggtc
    29941 aagtttgttc ataaaggtga ggatcggcgt gtcgcgcaga cgggtaactt ccatcagctt
    30001 acgggtacga tcttcaacac cttttgcggc gtcgataacc atcaggcagc agtccaccgc
    30061 cgtcagggta cgataggtat cttccgagaa gtcttcgtgc cccggggtgt cgagcaggtt
    30121 aaccaggcaa tcgtgatacg gaaactgcat cacagacgta gtaatggaga tcccacgctg
    30181 cttttccatc tccatccagt ccgacttagc gtgctggttg gaaccacggc cttttactgt
    30241 accggcggtc tgaatggcct gtccgaacag cagcaccttc tcggtgatgg tagtcttacc
    30301 ggcgtccggg tgagaaataa tggcaaaagt gcggcgcttc gccacctctt gcaaataagg
    30361 agacaacgtc ataattcttt cttcttgagt aagcggcatc gcgccgcgct tgttgaaaac
    30421 gaaaaattgc ggctatttta cccatcaacg ggggggaggc aatcagtgtt tacacaggag
    30481 ctgctccagt tcgtgcaacg aagaaacggt ccaggtgggc gcgatgcctt ctggttgctc
    30541 gcgattgtgc gcattcagcc agcaggtcgc aagcccggcg ttgatgccac cgagaatatc
    30601 ggactcagct gtgtcgccaa ccatcagcac gcgtgaacgg tcaggattgc ctgcctgttc
    30661 cagcgcataa tcgaaaattt tcttattcgg tttggcaacg ccaacttctt cggaaatcac
    30721 cagcaaatcg aaataatcac gcaggcccgt gcgttccaga cgcacttgct gcaaggcgct
    30781 aaagccgttg gtgatgatgc caattttagc gttgccacga atggcgttaa gcagagaaac
    30841 cgcgcccggc agtggcgtac agatttccgc catcgcatta ataaaggctt cgttgagttt
    30901 acccggctcg acgttcagcc gttcggccca gctctcaaac cgcccgtgct gaagctgtaa
    30961 tgaagtgatc gcgccgtttt gataatccac ccacagtggc ttgttaacgg cctgatagtc
    31021 ctgaaaatct tcagcggtaa aggtgacgct gtaatcaaga aacatccgct gcaggccggt
    31081 gaatgagtca aaggtaaaca gcgtttcatc ggcatcaaag aaaatccagt cccacttcat
    31141 tattccacct tgtcttacat actgattggc aacgccatga tgatggcgtc ttcgcgaccg
    31201 tccgtggtgg ggtagtaatt gcggcgaatt gtcgcctcgt taaagcctaa actttcgtac
    31261 agggcaatgg cggcagcgtt tgaagcacgg acttccagcc atagcgtcgc cacgccgcgt
    31321 ttttccagtt cgtcgatcag atgttccagc agcgcccttc ccaatccctg acgctgatag
    31381 tcaggatcga ccgcaatatt gaacaatgta gcttcatcca gcaccacttg cgtaatcgca
    31441 aacgccgcca ttttgccgtt ttgcgttaac tgaaagttga gataacgctc gccctggttg
    31501 ctggcaaagg ttttttcact ccacggaaag gcgtgggcgc gttgttcaat gtggtaagcc
    31561 gctggtaaat cagtcgtttc gagggaagaa atcgtgttca tatgtgcaaa tttgttgcca
    31621 taacgcggcg cgtgccgttg ggtttgcccg taattcggtg agcgccggtg atgccacctg
    31681 agcgccttcc agtgatagcg gttcgtcagt acccaaccgc cagctgttgc agcgactgcc
    31741 ttgcggcagc atcgcgattt tttctggcgt cagttgcagc acttgatcgg ggctgacggt
    31801 taacgcgcgc agaacatcgc tcactatagg atcggtcagg gcgggaagat cgtttgccac
    31861 catcaccaga cggacgtgtg ccgggatcgc aatggcaatc tccccctgca acgcgccagg
    31921 gcgacgcagc gaccactggg taatgcccag ttgctgtaac tgccagtctc gtcgggatgt
    31981 catagcgaat cgctcctgtt gtcaggggcg caaatatagc aaattcgtcg ataccgcgcc
    32041 aacatatggc tataatcgcc gccagtatta attgaggagc attccatgtc tgcatttacc
    32101 ccggcaagtg aagtcttgct gcgtcacagt gatgatttcg aacaaagccg tattcttttt
    32161 gccggagact tacaggatga cctgcccgcg cgtttagata ccgcggccag ccgtgctcat
    32221 acccagcaat tccaccactg gcaggtgtta agccgccaga tgggggataa cgcccgtttt
    32281 agtctggtcg ccacggcgga tgatgtcgca gattgcgata cgctgattta ctactggccg
    32341 aagaacaaac cggaagccca gttccagttg atgaatttac tttctctgct gccagtgggc
    32401 acggatattt ttgtcgttgg cgagaaccgc agcggcgtgc gcagcgccga gcagatgctg
    32461 tcagattatg cgccgttgaa taaaatggac agcgctcgtc gctgtggcct ctattttggt
    32521 cgtctggaaa aacagccagt atttgatgcc gataaattct ggggcgaata cagcgtcgat
    32581 ggcctgacgg tcaaaacgct gcctggcgtg tttagccgcg acggtctgga tgtcggtagc
    32641 cagttgctgc tctcgacgtt aaccccgcac acgaaaggta aagtgctgga tgtcggctgt
    32701 ggcgcgggcg tactttcggt tgcctttgcg cgccattcgc cgaaaattcg tctcactctg
    32761 tgcgatgtct ctgcaccagc ggtagaagcc agccgcgcaa cacttgcggc caacggtgtt
    32821 gaaggtgaag tctttgccag taacgtcttt tccgaggtga aaggtcgttt tgatatgatc
    32881 atctccaacc cgccgttcca cgatgggatg caaaccagcc tggatgccgc gcaaacgctg
    32941 attcgcggcg cggtgcgtca tcttaatagc ggcggcgagc tgcgaattgt agcgaacgcc
    33001 ttcctgcctt atccggacgt gctggatgag acatttggct tccacgaagt gattgcgcaa
    33061 accgggcgct tcaaggtgta tcgcgccatt atgacccgcc aggcgaagaa aggttgagta
    33121 tctgatatat gtcgccgcat accgtgtcgg atgcggcgta cacgccttat ctgaccgaca
    33181 aacgaattat ccacaatccg ctgatggaca tttttccagc aattacactg ctgtcgataa
    33241 ttaactattg acgaaaagct gaaaaccact agaatgcgcc tccgtggtag caattctttt
    33301 taagaattga tggtatgcga aggtggcgga attggtagac gcgctagctt caggtgttag
    33361 tgtccttacg gacgtggggg ttcaagtccc ccccctcgca ccaattatct ttacttcttt
    33421 tcttgtctct tcactgtttt atcacccctg attcaaattc attatcatca gcatgatgcc
    33481 agcaataagc gccaggcacg atggtagcaa aacgtaatgc cgtaactgtt tgtgatacag
    33541 catcaccggc gtcaatacca atcccatcag aatgacaatc cgccattgat ccggcgataa
    33601 atccgcatac atcagccctg caatcagcaa tcccggcagc aacactcccc agccactgcc
    33661 cagcgtacgt tgcaacatgg tctcatctct ttcattgata atgataacta atatcatatg
    33721 ataattttta tcatttgcaa gccagagaaa ttccttgcta tcgggtaaac ctatcgatag
    33781 gattagcaat aattatcatt tagattacta tcacgattat ggcctatcgt tccgcaccgc
    33841 tctatgaaga tgttatctgg cgaacgcatc tccagccgca ggatgctggg cttgcacaag
    33901 ccgtgcgggc aacgatcgcc gaacatcgtg aacatttgct ggagtttatc cgcctggatg
    33961 aacccgcccc acttaacgcc atgacgctgg cgcaatggtc atcaccgaat gcgctaagtt
    34021 ctctactcgc ggtctattcc gatcatatct accgcaacca acctttgatg atccgcgaaa
    34081 acaaaccgct tatctcactg tgggcgcaat ggtatatcgg cctgatggtg ccaccattaa
    34141 tgttggcgct gctgacgcag gaaaaagcat tagatgtgtc gccggaacat ttccacgttg
    34201 agtttcacga aaccggacgc gccgcctgtt tctgggtcga tgtgtgtgaa gataaaaacg
    34261 caacactaca ttcgccgcag cagcgaatgg aaacgttaat cagccaggcg cttgttccgg
    34321 tagtgcaggc attagaagcg acgggagaga tcaacggcaa acttatctgg agcaataccg
    34381 gttatttgat caactggtat ctgactgaga tgaaacagct gcttggcgag gcgacggttg
    34441 aatcgctgcg ctatgccctc ttttttgaga aaacgcttac taccggtgaa gataatccac
    34501 tctggcgtac cgtggtgcta cgcgacggcc tgctggtgcg ccgcacttgc tgccagcgtt
    34561 atcgcttacc ggatgtgcag caatgtggcg attgtacgct gaaatgaaag cactgccggg
    34621 gaagtaaacc cggcatcatg cggattaata aggatgcaac acattacttg tttcgcaatg
    34681 ccgcatacat aacagaatat ctgcggcctc caacaaccct gcatcagaac tgacgcccag
    34741 tttcgacatc acattaaatt tatgtgcacg gatcgttttg atattgcgtt taagctgctc
    34801 ggcaatttgt gtcattgagt agccacgcga cataaagcgc aatatttctc tctccgtcgg
    34861 gcttaacgtc cggctttggt taatgtacca ctgattgttc agtcgatcgg tcgcctgacg
    34921 tacaccattt aatgacaaaa agagttcctg atgaaaaatc tccagcgttg acgctttact
    34981 taatacaccg tccagcggtg atggcgataa tgaaccaatc agccgagctt caatatcatc
    35041 atccgcaata actaaacgcc gggtacgcgg aaacttaatc gccagttcag tcaggcagga
    35101 taatccctct ctgcgcgcac ttctcatggc agaaaaagaa aaaatgaccg ctgcaaacga
    35161 gatccgcgac atggcatcct gaaatgactc ctgactggta taggtatgga gcttataagg
    35221 gttacctgca aactttctga ataaactctc aataccgata ctactcatga cgcatttttc
    35281 tatgagtgcg acatttctct tcttaattcg gctgtgttcc attctgcggc atctccataa
    35341 acagaaggca agttaagttc tcttgcaccc tgcgcgctat ttatccatgc atacatttca
    35401 gcgttgctgt gaattgccag tcgacgcatc gcactgtttt tttgggcgct aatagtttta
    35461 ttacttttct taagcaatga agctatctgg ttgatgcccc atcctttacc taatagccgc
    35521 agtaccttgc gctctgaaag agtgagaatg acggagcgat agctaaaatc atgagtttca
    35581 gtcatcgccg gagaggtcag catggcgctg atacgctctg agtgcgtatt gccggaacgt
    35641 acggtcttga ccagattctc aatgggttca acatcagaca ataacgtcgc cgtagggcac
    35701 atgagcaatt ctactgcctg ggaataccag gagcgtgaca ccatgaaaac ccagtgaatt
    35761 tcccgatatt gtgagattaa agagtaataa tgctcacaaa cggaacgtgg atcttcactt
    35821 tcaccggcca aatcagcaat gactaatccg gaacgacgta attgtaataa ggtaaggtcc
    35881 tccgcagagg cgctggatat tatttcatat tcagggaagt gagtcctcat gacctctttt
    35941 aaccctgctt gcataacagg tattttactg ataacaattc catttttgca gcatcctggc
    36001 aacatatttc ctccacatcc actgtctaaa aatcatgatg gtttattcaa ggcattcatc
    36061 ccttaaatat ttccagcaaa ttaatagatt aagcgaataa attatcctta gcattcactt
    36121 tcatatcagc atactcctgc tgcacaacaa ctccactgca tgtgtcagaa ggttagcatc
    36181 aaatgataac ctggtgaaat agcctggaga catttaaaat atctacatta agttgttaga
    36241 aatacttaat gaaaaacaga aatctcattc atttaattta taa
//
LOCUS       DAZFJL010000029        36133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFJL010000029 DAZFJL010000000
VERSION     DAZFJL010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.029"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(1..399)
                     /locus_tag="ACHJ8C_001481"
                     /pseudo
     CDS             complement(1..399)
                     /locus_tag="ACHJ8C_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016190146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            976..1152
                     /locus_tag="ACHJ8C_001482"
     CDS             976..1152
                     /locus_tag="ACHJ8C_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5551363.1"
                     /translation="MKKTFTFSLLAIVFASLLSACVPHHHHRHNDGPRGPAASGKPMP
                     PSNGGPNHGSHGRY"
     gene            complement(1190..3109)
                     /gene="dhaR"
                     /locus_tag="ACHJ8C_001483"
     CDS             complement(1190..3109)
                     /gene="dhaR"
                     /locus_tag="ACHJ8C_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO5551364.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSVLGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAVAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTFPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDSENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDVSQFKRRG"
     gene            3337..4407
                     /gene="dhaK"
                     /locus_tag="ACHJ8C_001484"
     CDS             3337..4407
                     /gene="dhaK"
                     /locus_tag="ACHJ8C_001484"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO5551365.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEPQTKKPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            4418..5050
                     /gene="dhaL"
                     /locus_tag="ACHJ8C_001485"
     CDS             4418..5050
                     /gene="dhaL"
                     /locus_tag="ACHJ8C_001485"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO5551366.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVLEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQTTQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSCEQNLPVPAALDAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            5061..6479
                     /gene="dhaM"
                     /locus_tag="ACHJ8C_001486"
     CDS             5061..6479
                     /gene="dhaM"
                     /locus_tag="ACHJ8C_001486"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO5551367.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLSV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSIL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAETSGLDDIAAIFSGHHTLLDDPELQAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            6799..8496
                     /gene="treA"
                     /locus_tag="ACHJ8C_001487"
     CDS             6799..8496
                     /gene="treA"
                     /locus_tag="ACHJ8C_001487"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO5551368.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETSVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     ESEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPKPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILSRASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLMEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPLKQKEAEPTP"
     gene            8833..9855
                     /locus_tag="ACHJ8C_001488"
     CDS             8833..9855
                     /locus_tag="ACHJ8C_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFO5551369.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASRTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQTFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSIENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINHLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDVDY
                     QKLVAS"
     gene            9855..10835
                     /locus_tag="ACHJ8C_001489"
     CDS             9855..10835
                     /locus_tag="ACHJ8C_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO5551370.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILALVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PIAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            10832..11590
                     /locus_tag="ACHJ8C_001490"
     CDS             10832..11590
                     /locus_tag="ACHJ8C_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5551371.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTKKLAALYRVSADQIHHHLSAIKH"
     gene            11600..12412
                     /locus_tag="ACHJ8C_001491"
     CDS             11600..12412
                     /locus_tag="ACHJ8C_001491"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5551372.1"
                     /translation="MLIDDIDFADLYLQQLRLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTSKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALATASRDWALIWWDSVPQETLR"
     gene            12409..13263
                     /gene="modD"
                     /locus_tag="ACHJ8C_001492"
     CDS             12409..13263
                     /gene="modD"
                     /locus_tag="ACHJ8C_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFO5551373.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADAPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            13289..15259
                     /locus_tag="ACHJ8C_001493"
     CDS             13289..15259
                     /locus_tag="ACHJ8C_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO5551374.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(15309..15563)
                     /gene="ymgE"
                     /locus_tag="ACHJ8C_001494"
     CDS             complement(15309..15563)
                     /gene="ymgE"
                     /locus_tag="ACHJ8C_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO5551375.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            15764..16498
                     /gene="ycgR"
                     /locus_tag="ACHJ8C_001495"
     CDS             15764..16498
                     /gene="ycgR"
                     /locus_tag="ACHJ8C_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO5551376.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLNKAAIPLRISWNGGQLISKIL
                     VITPDKLVLDFGSQAEDNNAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRITAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMHFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(16500..17111)
                     /gene="emtA"
                     /locus_tag="ACHJ8C_001496"
     CDS             complement(16500..17111)
                     /gene="emtA"
                     /locus_tag="ACHJ8C_001496"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO5551377.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQIKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            17211..18125
                     /gene="ldcA"
                     /locus_tag="ACHJ8C_001497"
     CDS             17211..18125
                     /gene="ldcA"
                     /locus_tag="ACHJ8C_001497"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO5551378.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTQEGTQLTISGHPVLKM"
     gene            18220..19956
                     /gene="cvrA"
                     /locus_tag="ACHJ8C_001498"
     CDS             18220..19956
                     /gene="cvrA"
                     /locus_tag="ACHJ8C_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO5551379.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(20013..21083)
                     /gene="dadX"
                     /locus_tag="ACHJ8C_001499"
     CDS             complement(20013..21083)
                     /gene="dadX"
                     /locus_tag="ACHJ8C_001499"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO5551380.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPYARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARVDQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVLTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(21093..22391)
                     /gene="dadA"
                     /locus_tag="ACHJ8C_001500"
     CDS             complement(21093..22391)
                     /gene="dadA"
                     /locus_tag="ACHJ8C_001500"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO5551381.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLDSSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            22721..24253
                     /gene="ycgB"
                     /locus_tag="ACHJ8C_001501"
     CDS             22721..24253
                     /gene="ycgB"
                     /locus_tag="ACHJ8C_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO5551382.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            complement(24417..25136)
                     /gene="fadR"
                     /locus_tag="ACHJ8C_001502"
     CDS             complement(24417..25136)
                     /gene="fadR"
                     /locus_tag="ACHJ8C_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO5551383.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            25358..26899
                     /gene="nhaB"
                     /locus_tag="ACHJ8C_001503"
     CDS             25358..26899
                     /gene="nhaB"
                     /locus_tag="ACHJ8C_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO5551384.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRAEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRLQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            27045..27575
                     /gene="dsbB"
                     /locus_tag="ACHJ8C_001504"
     CDS             27045..27575
                     /gene="dsbB"
                     /locus_tag="ACHJ8C_001504"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO5551385.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALLFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWEFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(27620..28888)
                     /gene="umuC"
                     /locus_tag="ACHJ8C_001505"
     CDS             complement(27620..28888)
                     /gene="umuC"
                     /locus_tag="ACHJ8C_001505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO5551386.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVHNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADSDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFAFNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPCYTTRSSDLLRVK"
     gene            complement(28888..29307)
                     /gene="umuD"
                     /locus_tag="ACHJ8C_001506"
     CDS             complement(28888..29307)
                     /gene="umuD"
                     /locus_tag="ACHJ8C_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO5551387.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLTPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            29570..29893
                     /gene="hlyE"
                     /locus_tag="ACHJ8C_001507"
                     /pseudo
     CDS             29570..29893
                     /gene="hlyE"
                     /locus_tag="ACHJ8C_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            complement(30100..30546)
                     /gene="ycgN"
                     /locus_tag="ACHJ8C_001508"
     CDS             complement(30100..30546)
                     /gene="ycgN"
                     /locus_tag="ACHJ8C_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO5551388.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKGL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(30638..31297)
                     /gene="ycgM"
                     /locus_tag="ACHJ8C_001509"
     CDS             complement(30638..31297)
                     /gene="ycgM"
                     /locus_tag="ACHJ8C_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO5551389.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(31369..31662)
                     /locus_tag="ACHJ8C_001510"
     CDS             complement(31369..31662)
                     /locus_tag="ACHJ8C_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO5551390.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            31903..32304
                     /gene="pliG"
                     /locus_tag="ACHJ8C_001511"
     CDS             31903..32304
                     /gene="pliG"
                     /locus_tag="ACHJ8C_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO5551391.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(32407..32775)
                     /gene="ycgJ"
                     /locus_tag="ACHJ8C_001512"
     CDS             complement(32407..32775)
                     /gene="ycgJ"
                     /locus_tag="ACHJ8C_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO5551392.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSVQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            33295..33990
                     /gene="minC"
                     /locus_tag="ACHJ8C_001513"
     CDS             33295..33990
                     /gene="minC"
                     /locus_tag="ACHJ8C_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO5551393.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNISALEAPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            34014..34826
                     /gene="minD"
                     /locus_tag="ACHJ8C_001514"
     CDS             34014..34826
                     /gene="minD"
                     /locus_tag="ACHJ8C_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO5551394.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            34830..35096
                     /gene="minE"
                     /locus_tag="ACHJ8C_001515"
     CDS             34830..35096
                     /gene="minE"
                     /locus_tag="ACHJ8C_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO5551395.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(35468..35811)
                     /locus_tag="ACHJ8C_001516"
                     /pseudo
     CDS             complement(35468..35811)
                     /locus_tag="ACHJ8C_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(35846..35965)
                     /locus_tag="ACHJ8C_001517"
     CDS             complement(35846..35965)
                     /locus_tag="ACHJ8C_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO5551396.1"
                     /translation="MPLSYTGYHHFIMMSLMSGEHNTFALSLQDESPGLVPAG"
     gene            complement(35926..36111)
                     /locus_tag="ACHJ8C_001518"
     CDS             complement(35926..36111)
                     /locus_tag="ACHJ8C_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5551397.1"
                     /translation="MSRRYSLATITLVILFCGRPHCRKISVRQYYQKRVIFYPTTLQR
                     TAFKKYLFLIRDIIISS"
BASE COUNT         9048 a         9282 c         8789 g         9014 t
ORIGIN      
        1 agtttttcca agttatcagc ccatacttgc aaccatttcc tgcactcggc atcatactta
       61 tacaagttat aaatcccctc aacaccactg cgcgtataac cccgcacagc ctctgcaacc
      121 tcatttggac attgcaaacg catagccctg accgtactgt acgtagcaga ccatggggcg
      181 tccaataagg tatatcaaac atttgaccgc tttcacgtaa acgccatgag ttttagttag
      241 atacttctgc tgaattggct tcttagtgac ttgcgaagaa aacagatatt tgtcagaggt
      301 aaggcgtaga actttgagaa agtcggttgc ctgatcagat aactgtacgt agcgctgtac
      361 ccctgttttg gtttctcgca gatggatagt gtttttctca tgagcgacgc cccccccgtc
      421 cagtcgatct gacgtaacac atttatctgt tgtgctaaaa acagcaactg aacctcaccc
      481 aggcctcgag atgaaaaagt gtctgtaagg ccagaatctg tttgaggatg tgcacatggc
      541 actcagcgat gtaatgctaa ataagtatgc cagtaagtac gcgggaaaga atacagtgag
      601 gttatataca gataaaaaac tgtaactaat tgatttaaat ggtgcgataa taggagccga
      661 tccttcgcat tacgaaagcg agccgactcc aaaatgagta caagaatgag tatgctgaaa
      721 ataaaatttt taataaaata taaatatcaa taagataaaa ttaaaatcac ggttcaacgt
      781 ctaattccgc taatactacc ctggcggtta ataacgagcc attatgtgag ttgcacgtaa
      841 tggctttttt acgcctgcta cgcgtcttct ttcaattctg agttaccaaa actcataatc
      901 agactcctga caatattaac aatgcagctt ttatgtgata acaatcacag tgcattaacc
      961 gttcaagagt ttgtcatgaa aaaaacattt accttcagcc tgttagcaat tgtctttgca
     1021 agcctgttga gtgcttgtgt tcctcaccat catcaccgcc acaacgatgg cccgcgtggt
     1081 cctgcggctt ccggtaagcc aatgccacct tcaaatggtg ggccaaatca tggcagtcat
     1141 ggtcgctatt aattagcaag tcatacgcgg gagagtgcat actctcccgc tatccacggc
     1201 gcttaaactg actcacatca atgccatgtt gcttcatttt ccgccacagc gtggtgcggc
     1261 cgattccaag taaagccgac atttcctgaa tgcgaccgcc tgtgacctgg gctgcgttaa
     1321 taattgcctc tttttcaact tccgcaaatg acagactggt ggaaaggcgg gtggcgctga
     1381 catcatctgt cgcctgctcg gtaaacagat gttccggcaa atcactgacg cgaatgcgcc
     1441 cattatcact actcagagca agattctcga tgacgctgta aagttcaaaa tcgttgccag
     1501 gccatgcaca ggaaaccagg cgagcgaggg catcgtcatc aattttcagc cgcgtagaga
     1561 agcgtttttc aaggctgcgt aatttgttat tcaccagcgc cggaatgctg ccacgccgca
     1621 tacgcagtgg cgggatggta atttcaaatg catgcagcgc gtaatacagc tggcgactaa
     1681 aacgattttg ttccaccagc attgcgaggt ccgcggtcgt tgtggcaatc actttgacat
     1741 caatgggtat taaacgccgc gcatccagtc gcgtgataac cccctgcttg ataacctgaa
     1801 gcaaggcaga ctgtaactcc accgccagat attcaatctt ttcaagaaac agcgtgccgc
     1861 cgtgcgccag ttccagccga ctcagacggc cattttcact atccgtgcga tcgccaccaa
     1921 taaattcttc cgccagcgcc gcatcaccat ataactcaca attgacggcg atataaggcc
     1981 ccgctgcacg ctcgctttca ttatgaattg cctgacttag cagtgccttg cccacgccct
     2041 cttctccaca aagcaggaca ggaaagctac tgcgcgccgc ctggcgacca aaatgaatca
     2101 agcggcgggt ttgcggatcg tcctgtggca tatgagcgaa ggtatggctg acttttccta
     2161 attgactggt cattaactgc cgcatctgtt ccacaggatg gagcaacaaa ataaagctgg
     2221 ttccctgtgt ttctattatc ggtttaaggg ttatcaccgc atcaataaac tgatgctgac
     2281 tttcaaaggt ggcttctacg tgtttgagcg gatgtgcctg ttttattgct tgttgcaata
     2341 cggcggggaa cgttaagagt tcagtgattg ctcgtccctg acttgccgtc gcgtcaaggc
     2401 gcaagactcg cgccgcctgg gcgttgataa attgcaaatt accctgctcg tcccagctaa
     2461 tcacgccatc atccatactt tctaacaagg cattaagttg atttaaatga cggttagttt
     2521 cagcgagcaa actgtccgtc agcagtaaat ttccgacctc gcgtgcgact gccaacgtca
     2581 acggcaaatc agctgcggta gtttgctcaa ccggacacgc cagcgctatt gttcccgtca
     2641 atcggccctt gctgtcaaac aacggcgttg cgcaaaaggc ccagttccag agtgcctgtt
     2701 tgaaatgttg atcggccatc gttttcacgg cctgaccaga gatagccgct aacgatagcg
     2761 cacaagtacc aataattccc tcggcgcaat acgtgccgtc attgaatccc agtacactta
     2821 gctgctgcaa ggtttgcgga tcgccattac ggctgagaat gcaggcggtt tcatcgagga
     2881 taagcaacgc acactctcgc ggtgccatat attcccaggc atcttccagc gcagcctgcc
     2941 cgagcgtcag catcgctttc ttacgccgat aaatagaagc aaatgtcacg ccctgggcct
     3001 ggtgtggtac gttccatgtc tcccgtttca tcagcttatt gcatctctcc caggaggttg
     3061 caattaaggg agatatgccc cgaccatcgt tgttaaaagc gccactcata tcccgcatcc
     3121 cttatgacaa aaggtgttcc ggctcgcaat tttaagtatt aatacacgta aaagtttcaa
     3181 tatggaacat tcattaaagc atatgtttca ctatgaaacc taatttgggc taattttctt
     3241 ttcccttgcc tgatgcacaa tggattcatc ggcagtcagt ggtcgccgtg tcgttgaaca
     3301 tcatccatgc cctaccgtaa ttgctggagc aaaataatga aaaaattgat caatgatgtg
     3361 caagacgtac tggacgaaca actggcagga ctagcgaaag cgcatccatc gctgacactg
     3421 catcaggatc cggtgtatgt cacccgagct gatgcccccg ttgcaggaaa agtcgccctg
     3481 ctgtcgggtg gcggcagcgg acacgagccg atgcattgtg gttatatcgg tcaggggatg
     3541 ctttctgggg cctgtccggg cgaaattttc acctcaccga cgcccgataa aatctttgaa
     3601 tgcgccatgc aaattgatgg cggcgaaggt gtactgttga ttatcaaaaa ttacaccggc
     3661 gatattctta acttcgaaac agcaaccgag ttactgcacg atagcggcgt aaaagtgacc
     3721 actgtggtca ttgatgacga cgttgcagta aaagacagtc tttataccgc cgggcggcgc
     3781 ggcgttgcca acaccgtatt aattgaaaaa ctcgtaggtg cagcggcgga gcgtggcgac
     3841 tcactggacg cctgtgcgga actggggcgt aagttgaata atcaaggcca ctcaataggt
     3901 atcgctctcg gtgcctgtac cgttcctgcc gcgggcaaac cttcttttac cctggcggat
     3961 aatgagatgg agtttggcgt cggcattcat ggtgagccag gtattgaccg ccgccccttc
     4021 tcttcccttg atcaaaccgt cgatgaaatg ttcgacaccc tgctggaaaa tggctcatac
     4081 catcgcactt tgcgtttctg ggattatcaa caaggcagct ggcaggaaga accacaaacc
     4141 aaaaaaccgc tccagtctgg cgatcgggtg attgcgctgg ttaacaatct tggcgcaact
     4201 ccgctttctg agctgtacgg cgtctataac cgcctgacca cacgttgcca gcaagcggga
     4261 ttgactatcg aacgtaattt aattggcgcg tactgcacct cactggatat gaccggtttc
     4321 tcaatcacct tactgaaagt tgatgacgaa acgctggcac tctgggacgc cccggtccac
     4381 accccggccc ttaactgggg taaataagga gaaagcaatg tcactgagca gaactcaaat
     4441 tgttaactgg ctcactcgtt gtggcgatat tttcagcacc gagagcgagt atcttactgg
     4501 actggatcgc gagattggcg acgctgacca cgggttaaat atgaatcgag gctttagcaa
     4561 agtgttggaa aaactccctg ctatcgcaga taaagatatc ggtttcattc tcaagaatac
     4621 cggcatgacg ctactttcca gcgtcggagg tgccagtggt ccgctgttcg gtaccttctt
     4681 tatccgcgcc gcacagacga cccaggcacg gcaaagcctg acgctggaag agctttatca
     4741 gatgttccgc gatggcgcgg acggcgtaat cagtcgcggg aaagccgaac ctggcgataa
     4801 aaccatgtgt gatgtgtggg tgccggtggt ggaatcatta cgtcagtcct gcgagcaaaa
     4861 tctccctgtt ccggcggcgc tcgacgctgc cagtagcatc gccgaatccg ctgcacaaag
     4921 tacgattacg atgcaagccc gcaaaggccg cgccagttat ctcggtgaac gcagtattgg
     4981 tcaccaggat cccggcgcga cctcggtgat gtttatgatg caaatgttgg cgttagccgc
     5041 aaaagagtaa ggaattagtg atggtaaacc tggtcatagt ttcacatagc agccgactgg
     5101 gagaaggtgt cggtgaatta gcccgtcaga tgttaatgag tgataattgt aaaatcgcca
     5161 ttgccgcggg aattgacgat ccacaaaatc ccattggtac cgatgccgtc aaagtgatgg
     5221 aggccatcga atctgttgcc gatgccgacc atgtgctggt catgatggat atgggtagcg
     5281 cattattgag tgctgaaact gcgctggaat tgctggctcc cgagatcgcc gcaaaagtac
     5341 gtttgtgtgc tgcgccgttg gtcgaaggta cactggcagc aacggttagc gcggcctcgg
     5401 gggcggagat cgacaaagtg atctttgacg ccatgcatgc gctggaagcc aaacgtgaac
     5461 aactgggttt accgtcctcc gacactgaaa tctctgacac atgtcctccg tacgatgaag
     5521 aagcccgttc tctgtcggtg gtcataaaaa accgtaacgg cctgcatgta cgtccggcct
     5581 cccggctggt ttatacctta tcgacattta atgccgatat gttgctggag aaaaacggca
     5641 aatgcgttac accggagagt attaaccaga ttgcgttact acaagttcgc tataacgata
     5701 cgctgcgcct gattgcgaaa ggaccagaag ctgaagaggc actgatcgcc ttccgtcagc
     5761 tggctgaaga taactttggt gaaacggagg aagttgctcc acctactctg cgtcccgttc
     5821 cgcctgtttc gggtaaagcc ttttattatc aacctgtttt atgtacggta caggcaaaat
     5881 caatcctgac cgtggaagaa gaacaagaac gattacgcca ggccattgac ttcacgttat
     5941 tagatctgat gacgttaaca gcgaaagcag aaaccagcgg gcttgacgat attgccgcaa
     6001 tcttttctgg tcaccataca ctgttagatg atccggaact gcaggcggcg gcaagcgaac
     6061 tccttcagca tgaacattgc acggcagaat atgcctggca gcaagttctt aaagaactta
     6121 gccagcaata ccaacagctg gatgatgaat atctacaggc tcgctatatc gatgtggacg
     6181 atcttctgca tcgcaccctg gtccacctga cccaaacgaa agaagaactc ccgcagttta
     6241 actcgccaac tattctactg gcggagaaca tttatccctc cacagtactg caactggatc
     6301 cagcggttgt aaaaggtatt tgccttagcg ccggaagtcc gctatcccac agtgccctaa
     6361 tcgcccgtga actgggaatt ggctggattt gccagcaggg tgagaaactg tatgcgatac
     6421 aaccagaaga aacgctaacg ctggacgtta aaacgcaacg tttcaaccgt cagggttaaa
     6481 ccaccgttcg gatggcatca ttctgatgtc atccggcaat ttactctgca ctgatgtttt
     6541 ttaactacct gttatatttt tcaccaaatt atgatgattt ttcttcgcta atcgcgaacc
     6601 caacgtcgct ggctgactaa taaacagatg ttattttatc actctatttc gccgttattt
     6661 atccctttaa ttcccttttc taaaatgtct gtcagttcgc agaatgtgat ttcgatcatg
     6721 cagctagtgc gatcctgaac taaggttttc tgatacttga ataccgtttt attcggtttc
     6781 gccaaaggag aatgattgat gaaatccccc gcaccttctc gcccgcaaaa aatggcgtta
     6841 attccagcct gcatcttttt gtgtttcgct gcgctatcgg tgcaggcaga agaaacatcg
     6901 gtaacaccac aaccgcctga tattttatta gggccgctgt ttaatgatgt gcaaaacgcc
     6961 aaactttttc cagaccaaaa aacctttgcc gatgccgtgc cgaacagcga tccgctgatg
     7021 atccttgctg attatcggat gcagcaaaac cagagcggat ttgatctacg ccatttcgtt
     7081 aacgtcaatt tcaccctgcc gaaagagagc gagaaatatg ttccgccaga ggggcaatca
     7141 ctgcgcgaac atattgacgg actttggcca gtattaacgc gttctaccga aaacaccgaa
     7201 aaatgggatt ctctattacc gctgccaaaa ccttatgtcg tgccaggcgg acgttttcgc
     7261 gaggtatatt actgggacag ttacttcacc atgttaggac ttgccgaaag cggtcactgg
     7321 gataaagtcg cggatatggt ggccaatttt gctcatgaaa tagacactta cggtcatatt
     7381 cccaacggca accgcagtta ctatttaagc cgctcgcagc cgcccttctt tgccctgatg
     7441 gtagagttac tggcgcagca tgaaggcgac gccgcgttga aacaatacct gccgcaaatg
     7501 caaaaagaat atgcttactg gatggacggt gttgaaaacc tgcaagccgg acaacaggaa
     7561 aaacgcgttg taaaacttca ggatggtacc cttctcaacc gctactggga cgatcgcgat
     7621 acgccacgac cagagtcatg ggtggaagat attgccactg ccaaaagcaa tccgaatcga
     7681 cctgccactg aaatttaccg cgacctgcgc tctgccgctg cgtctggctg ggatttcagc
     7741 tcgcgctgga tggacaatcc gcagcagtta aacaccttac gcaccaccag catcgtaccg
     7801 gtcgatctga acagcttgat gttcaaaatg gaaaaaatcc tctcccgcgc cagcaaagct
     7861 gccggagata acgcgatggc aaaccagtac gaaacgctgg caaatgcccg tcaaaaaggg
     7921 atcgaaaaat acctgtggaa cgatcaacaa ggctggtatg ccgattacga cctgaaaagt
     7981 cataaagtgc gcaatcagtt aaccgcggcc gccctgttcc cgctgtacgt caatgcggca
     8041 gcgaaagatc gcgccagcaa aatggcgacg gcgacgaaaa cacatctgct gcaaccaggc
     8101 ggcctgaaca ccacgtcggt gaaaagtggg caacaatggg atgcgccaaa cggctgggca
     8161 ccgttgcagt gggtcgcgac agaaggatta caaaactacg ggcaaaaaga ggtagcgatg
     8221 gacattagct ggcacttcct gaccaatgtt cagcacacct atgaccggga gaaaaagctg
     8281 atggaaaaat atgatgtcag caccaccgga acagggggcg gcggtggcga atatccatta
     8341 caggatggct ttggctggac caatggcgtg acgctgaaaa tgctggattt gatctgcccg
     8401 aaagagcaac cgtgtgacaa tgttccggcg acgcgtccac tcagtgaatc aacaacacag
     8461 ccgctaaaac aaaaagaggc ggaacctacg ccttaacttt actgtatatc gagcaatgcc
     8521 attacgtgca taaaattggc attgctcttt cttcatcacg aaataccaaa taccactctt
     8581 tctacggtct atgcctttat tttcttttat tagagaagat tttaatttta ttttatcaac
     8641 ttgttaatca ttacacctct atttatattt tcccccactc cccattctcc ctgacaattt
     8701 tcttgtggaa caaagtgtta tttaagaaac aagcaaatca ttctgtctct ttagacaaat
     8761 tttgacatca aacatgatct ttttcgctat atgtgtaagt atatagcggt tttaattatc
     8821 aggatattac ttatgtcact aaccagacgg aggtttacac agattcttgc gtcgacattg
     8881 ttcctgcatc atctgccgtc ctttgcacaa tcagtcaaat tctgggcctc acggacgctt
     8941 cccgaagccc aaaacattac acgcatcgtc agcgcaggcg cgcccgccga tttactattg
     9001 ctggctgtcg cgccagaaaa aatggtcggt ttttcctctt ttgattttgc ccgtcaggca
     9061 ttaattccat tgccagagca cattcgccag ttccccaggc tgggacgact cgccgggcgc
     9121 gccagcacac tctcgctgga agggcttatg gcattacatc ccgatttggt cgttgattgc
     9181 ggcaatacgg atgaaacctg gatctcccag gcacggcagg ttagcgaaca gacacaaata
     9241 ccctggttat tgcttaacgg gaaactggaa caatcagcag aacagttaac aacgcttggc
     9301 aaaacgttag gcgaagagca ccgcgccgca gaacaagcca atctcgccag ccgcttcgtt
     9361 ggtgaagctc agacattcgc cacctcaccc gccgctaacc tcagctttta tgctgcgcgc
     9421 ggtcctcgag ggctggaaac gggcttacag ggttcgttgc ataccgaggc ggcggaatta
     9481 ttaggtttgc acaacgtcgc gcaaatagcc gatcgccacg gtctgacaca ggtttccata
     9541 gaaaatctcc tgcgctggca gccggatatt attctggttc aggaggccgt tactgcagat
     9601 tttattcgtc gtgatcctct ctggcagggc gtgaaagcgg ttgcggaaca acgcatctta
     9661 tttttaagtg gcctgccctt tggctggctg gatgcccctc cgggaatcaa ccatcttctg
     9721 ggattacgca gacttcacgc ctggctggat cccgccatca atcgccagtt taaaagtgac
     9781 atgcagcatt acgcccaact gttctggcat tgttcactca gtgacgtcga ctatcaaaaa
     9841 ttggtggcga gctaatgaga accgtaaacg gatgtatttt gctgctggca gctatcagta
     9901 tcacttttgc cgccgtaagt ggggcttatc atctcgatat gcagcaactg ctggcgctca
     9961 ttcttcgtca agaaaatgtt cctgtgcagg aacaaattgt cttctggcaa atccgcgtgc
    10021 cgcgtattct tgctgcgctg tttctgggcg cggcgctggc aggggccgga accacctatc
    10081 agggaatgtt ccgtaatccc ttagtgtcac cggatatctt aggcgtctcc gccggtgccg
    10141 gacttggtgc ctgcgcggca atcctgtggg gattatccat tgtttacatc cagctgtacg
    10201 cattttgcgg cgggctgatg gtggtcgcgg gcgtctggtt gattacgcgt cgggtgaccc
    10261 gccacgatcc gatcttagcc ctggtgctgg taggcattgc gttaggaacg ttgtgcggag
    10321 cggggatatc gctaataaaa acgctggccg atccttacac ccagttaccg tcaattacct
    10381 tctggttgct gggggggctg tccaccgtga ccttacgcga tctctgctat gctgcaccga
    10441 tcattctcat aggctccttg cccctgtttt ttctccgctg gcgcatgaat ttactcacgc
    10501 tttccgacga cgaagcccgt tcgctgggac tgaacgtgac acgtctgcgc tttggattga
    10561 ttgtctgcgc cacactcatc accgccagca cggtagcaat agcgggcata attggttggg
    10621 tcggcctggt cgttccgcac attgcccgct tgttgaccgg tcataaccat cagcagctgt
    10681 tgcccatagc aatgtgtact ggcgcgattc tgctcctgct taccgatacg ctggcacgca
    10741 gcattggaac aacagaaatc ccacttggca ttctgaccgc ttttgttggc gcaccgtttt
    10801 tcctgttatt gttgttacgc ggaggtcgcc agtgactttg cttgccgtcc gtcatgcgtc
    10861 tctgggttac tcccgccatc cggtgctacg cgacgtgtcc ttcaccctgt cgcaaggaac
    10921 aatttgttgt ctgttaggag ccaatggttg cggcaaaacc acactcatgc gctccattct
    10981 tggcgttata ccgttattaa aaggcgagat cttactggat agcctccctg tgcagacact
    11041 cagccatcgc cagcgcgcgc aggctattgc ctgggtgccg caggcgcacg acggcatttt
    11101 tgcttttagt gtgctggata tggtgttgat gggcctggcc cccaccatag gcgctttttc
    11161 cgttcccggt aagcaagaac gcctgaaagc aacggagcaa ctggaaaagc tgggcatttt
    11221 gcatctggcg acacgacgct ggaatacgct tagcggcggt gaacgtcagc tggctttaat
    11281 cgcccgtgca ctcgtccagc aaccgcgctt gttattactg gatgagcctg cctccagcct
    11341 cgatttcggt catcagatcc aacttctgga cacgctggcg cagctcaaaa ataacggcat
    11401 gacaatgtta atgtcgactc atcatccgct ccatgcaaac gccattgccg acagcattat
    11461 ccaggtagag ccagatggac gagtaacaca aggcttaccg accgaacagt taacaacgaa
    11521 aaaactggct gccctgtacc gggtttctgc cgaccagatt caccatcacc tctctgcaat
    11581 taagcactaa ggaaaaatca tgctcattga cgatattgat tttgcagacc tttaccttca
    11641 gcaactcagg ctggcccatc gcacagaaaa aacgccggat cactgggatc aacgggcgga
    11701 aaaaatggcg gaaaattgcg cctcgcctac ggacagctac ctgcaacaac tgacctcaaa
    11761 aattgacctg caaggcgcgc aaaccttatt cgatatgggc tgcggaccgg gtaccgtatc
    11821 gctggcgctg gctgacaaac tcacgacgat ttatggcgtt gattacagtc aggggatgct
    11881 gaacgttgcc gcccgtcgcg ccgccgcgct aaaggcagac aatgtccact ggatccagcg
    11941 cgcctgggaa gaggactgga gtgacctgcc acgctgcgat attgccgtgg cttcccgctc
    12001 cactctggtg gctgatatgc gccaggctat gagcaaactg aataatcagg cacggcttcg
    12061 cgtctatacg actcatctgg tcagcacctc gttcgtctct ccagccattc agcgggcggc
    12121 tggtcgggaa gtgatcgaat tacccaacta tatctttgcg ctcaacgtcc tgtatcagat
    12181 gggaatttat gctcacgttg attttatccg cggacaaaat tgccaacagg ataacagcac
    12241 ctgggaacgt tttgagcaga acgtcagctg gagtctgggc gcgcttaacg acgatgagcg
    12301 tgagcggtta tatcgctggt atcaacaaca agatgctcgt gccctcgcca ctgccagccg
    12361 cgactgggcg ctcatctggt gggatagcgt accgcaggag acactgcgat gattttcctt
    12421 tctcaggcac aaatcgatgc gttactgctg gaagatatcc agggcggtga cctgaccacg
    12481 cgagcattaa atattggaca ccagcatggc tatatagagt tttttctccg tcagggcggc
    12541 tgcgtcagcg gcatttctgt cgcgtgtaag atgttaacta cgctgggatt aactattgat
    12601 gacgcggtca gcgacggttc acaagcgaac gcgggtcagc ggctaatccg tgcgcaaggt
    12661 aatgccgcag cacttcatca gggatggaag gcagtccaga atgtgctgga gtggagttgc
    12721 ggtgtttctg attatctcgc tcaaatgctg gcgttacttc gtgaacgtta ccctgatggc
    12781 aatatcgcct gcacccgaaa agcaattccg ggtacccgct tactggcctc gcaggcaatt
    12841 ctggctgccg gaggactgat tcatcgtgcc ggatgtgcgg aaaccatatt attgtttgcc
    12901 aaccaccgcc attttcttca tgacaatcag gactggtcag gcgcaatcaa tcagttacgt
    12961 cgccacgcac ccgagaagaa aatagttgtt gaagccgacg cgccgaaaga ggcaatcgcc
    13021 gcgttacgcg cgcaaccaga cgtacttcag ctcgacaaat ttagtccgca acaggcaact
    13081 gaaattgcgc aaatagcacc gtcgctggct ccccactgca cgctggctct gaccggcgga
    13141 attaatctga caacactcaa aaattacctc gactgcggca ttcgcctttt tattacctcc
    13201 gcgccttact acgcggcacc tgctgacatt aaagtcagtc tgcaacccgc agcctctatt
    13261 taattctttg ttaaacggaa tttattctat gagactcaaa aaacgttatc tctgcacagc
    13321 attatcactt gcctttaccc agcaggccgt agcagctcag gagagcgaca cgctgaccgt
    13381 atggtccagt ccggtatcat caacgacgac caccgttctc gatcaaccca ccatgaaggc
    13441 cctggataaa cagaatgtcg ctcaggcatt aagtgtcgtc cccggcgtgg tactgcaaaa
    13501 gtcaggtagc cgcaacgaag aacaggtcaa agttcgtggc tttgatagtc gtcaggtgcc
    13561 ggtctatttc gacggtgtgc ccatttatgt tccctatgac ggcaacctcg atctggcgcg
    13621 gattctgacc aacaatttgg gagcagttga agtttccaaa gggtattcgt cgctgcttca
    13681 ggggcctaat cagatgggcg gagccattaa tatcaccacc cagaagccaa caaaacctct
    13741 ggaagccagt ctgggatatc gccagggatg gagccgtagc caggacaatg cctacgatat
    13801 gcatgcttca tttgccgcca gcagcgatct ggggtatttg caagtcagcg gtagccagct
    13861 aaagcaggat tttctcggcc tgccgcatgg cgtaaataat gatattgcag gcaaacacgg
    13921 caagatgatt aattcatcgg ctgatgataa acgcggcatt gtgaagctcg gttttacacc
    13981 acgcgaaaac gatgaataca cattgactta cattaagcag gatggtgaaa aagataaccc
    14041 gccatacagt ggaaatagtg gtcagaaatc acgctactgg cagtggccag agtatgacaa
    14101 agaaagtttt tattatcagg gaacgaccca actaaacgat cgttttaccc tgaaaagtcg
    14161 gctgtatcgc gacacctttg aaaatacgct aatgatgtac aactcgctgg ctgatttgaa
    14221 aaataaaaaa ggcagctaca gccattattc cgattacagc gacggtgccg gattacaact
    14281 ggcagccgat gtgcgtgaaa acgatctgct gtcgtttgcc gttaactgga aagatgacgt
    14341 acaccgggaa aaaggtgcgc cgcacgccgc ttacgatcgc tatgaagatc gtacctggtc
    14401 gctcgccagt gaatatcaat gggctgctgc cgataatgtc gatgtcgtgg ctggaatcag
    14461 ctatgactgg cgcgatagcg tagaagcgaa aaaacatgag aaagatggca gtatcaccca
    14521 ttatgacgac aacaatcagt cagcttttaa ctggcaggtg atggggaaat accactttgc
    14581 caatgaagac acgctggcgc tttcgtacta tgaccgcaca cgctttccga cgctgaaaga
    14641 acgctatacc acgtccaaac ctgcgtataa ccagatagcg attgttaacc cgcagctcaa
    14701 gccggaacgc gcgcgcgggg tggatttaac ctggaatggt gccttcacgc acgactgggg
    14761 atttgaggtc agcgtttact ataaccgggt gagtgatgcc atcctctcgc acaatatcga
    14821 tgccgatacc attcaaaatc agaacagcgg cacggtggat tacagcggtc tggatgccgg
    14881 tattaagggg aaaatcagca atatactgga tgtaggattg agctacgccc tgatccacgc
    14941 tgatgccaaa cgtaaagaca tcggcaagat aaccgatctg ccaacgcaga caatgaccgc
    15001 atggatgact ctcaaaccgt gggagccgtt aagcgtaacg ctgtcggaag aggcgcgttc
    15061 ctccagctac agcaacagtg acggttcaca aaaagccgcc ggttttgcgg tgacccacat
    15121 tcgagccgat tacaccttag gtcatggctt cagcgttaat gcgtcggtca ataacctgtt
    15181 tgataccaaa tatgcctaca gtgaagggtt tattgaagaa ggtcgcaatt tctgggctgg
    15241 tatcgagtat acgttctgat gtgaaagcgg atagcggcat ctgtatcgct atccgcctta
    15301 tgaaaatctt attctcttcg caggaggcgg aatacgccca gcaccaggat agcgcccacc
    15361 accgccacca agaagctgtg caaattaaaa ccgctgatgg agccgccaat gccaaacatg
    15421 gtcgccagcc agccgccgac caccgcaccg actatcccaa gaatacaggt caggaaaaat
    15481 ccaccaccat cacgccccgg catgattagc ttggcgataa taccggctat caggccaaaa
    15541 ataatccagg caataattcc catgttcagg ccctcgtttc gatgaatcgg acgcaagaaa
    15601 tgcttacgtc aacgctctaa gtataggcaa gggcgggatc gatgctgggc gtatgtcggc
    15661 tgatttaaga acaacatgta aaaaatattc gcgcatttgt attaagtttt gttaactgcg
    15721 accgataaac caaagacagt ttgtcagtca ggagtttttc cgcgtgagtc attaccatga
    15781 gcagttcctg aaacaaaatc cgttagccgt cctgggcgtg ttacgcgatt tgaacaaagc
    15841 cgcaattcct ttgcgtatca gctggaatgg cgggcagctg atcagcaaaa tactggtaat
    15901 aaccccggat aaactggtgc tggatttcgg cagccaggcc gaagataaca acgccgtgct
    15961 aaaggcgcag cacattacca ttaccgccga aactcagggt gcgaaagtcg agtttactgt
    16021 tgaacaacta cagcagagtg aatacttgca gcttccggca tttattaccg taccgcctcc
    16081 caccttatgg tttgtacaac gacgccgata tttccgcatc accgccccac tccatccgcc
    16141 ttatttttgc cagaccaaac tggcggataa cagtacgtta cgtttccgcc tgtatgattt
    16201 gtcgttaggc ggcatgggcg cattactgga aacagcaaag cctgccggat tacacgaagg
    16261 catgcacttc gctcagattg aagtcaacat ggggcaatgg ggtgtttttc actttgacgc
    16321 ccagttaata tccatcagcg agcgcaaagt gattgatggc aaaaatgaaa ccatcaccac
    16381 tccccgtctg agcttccgtt ttcttaacgt cagcccgacg gtggagcggc aattacagcg
    16441 gattattttc tctctcgagc gggaagcacg ggagaaagcg gacaaagtgc gcgactgatt
    16501 tacatcgcgt ccagtgcctg ctcaagtttg tagatatagc gcggagcctg tggcgcagga
    16561 tgatttcgcg ctacgtgatc gaggaactcg tcagcatcca gatcgttgat tttgctgatc
    16621 gcctttttcc gatctgacga gaaagtccgt agcagcgcac ctgccccgtt agcgtatgac
    16681 accaccagcg catattgcag taccttcgga tcttcaatgc ctgccagcgg gccggtttcc
    16741 agaatattca ggtaagccgc ccccattgaa atattacgct ccggattttt cagctcgctg
    16801 gtcgtcggct caccactcca gcccatacga cgatatacat cacgtccgga ggttgaagct
    16861 tttatctgca tcaaaccaat ggcattcgat ttactcaccg cgttcggatt accacccgat
    16921 tcgatagcga taatcgccgt gatcaattgt ggatcgacgc cccaggctgc accggctttc
    16981 tggcttattg gcatccactg catcgcacgt tgcaccggaa ctttcgcgtt ccacggcggg
    17041 ttcgtatagt catgctttga tgaacaaccc gctaataaca caatcaaaaa ggcaaaccat
    17101 ctcaatttca cttcttctat ccttatagcc tggaatcgcc gttgaggtga caacggcaca
    17161 cctaagcggc atgatataca tttcgtttct gttgtcagca aggaattgcc atgtctctgt
    17221 ttcacttaat tgccccatcg ggttactgca ttaaacagca cgccgcgctg cgtggtatcc
    17281 agcgccttac cgacgcgggg catcaggtca ataacgtcga ggttattgcc cgtcgttgtg
    17341 agcgttttgc gggtacggaa acggagcgtc tggaagatct caattctctt gccagactga
    17401 ctacccccaa caccatcgtg ctggctgtac gcggcggtta cggtgccagt cgtttactgg
    17461 cagatattga ctggcaggca ctggtggctc gccaacaaca tgacccgttg ctcatttgcg
    17521 gacatagcga ttttaccgcc attcagtgcg gtcttctggc gcatggcaat gtcatcacct
    17581 ttagcggccc catgctggta gcaaactttg gcgcggatga actgaacgcc tttaccgagc
    17641 accatttctg gctggcgtta cgcaatgaaa ccttcaccat tgaatggcaa ggggaaggcc
    17701 cgacatgtca gaccgaaggc acgctgtggg gaggcaatct tgcgatgctg atttcactga
    17761 ttggtacacc gtggatgcca aaaattgaga acggtattct ggtgctggaa gatattaacg
    17821 agcatccttt ccgcgtcgaa cgtatgctgt tgcagctcta tcatgctgga attttgccac
    17881 gtcagaaggc gattattctc ggtagcttta gcggcagcac gcctaatgat tatgacgcag
    17941 gttacaacct ggagtcagtg tacgcgtttt tgcgttcccg cctgtcgatt ccactcatta
    18001 ccggcctcga ttttggtcat gaacaacgca cggttacgct gccgttgggc gcacatgcca
    18061 ttctgaataa cacccaggaa ggcactcagt tgacgattag cggtcatcct gttcttaaaa
    18121 tgtaaaaaaa tcacgcttca gggctaagta aaacgctgtc tctgccgcta atatggtagg
    18181 gtttatataa gagacagcgt aatcaggagt aaccgaccgt tggatgccac aacaataatt
    18241 agccttttca tcttaggatc catccttgtc accagcagta ttttacttag ttcattttct
    18301 tcccgtcttg gcattcccat tctggttatt tttctggcga tcggcatgct ggcgggggtt
    18361 gatggcgtcg gcggtatccc gtttgataat taccccttcg cctacatggt aagtaacctg
    18421 gcgctggcga ttattttgct cgacggtggg atgcgcactc aggccagttc ctttcgtgtg
    18481 gcgttaggac cggcactgtc gctggcgacg ctgggcgtac ttatcacctc tggtttaacc
    18541 ggcatgatgg cggcgtggct gtttaatctt gatttgattg aaggattatt aatcggcgct
    18601 atcgtcggct ccaccgatgc tgcagcggtc ttttctctgc tgggtggtaa ggggcttaac
    18661 gaacgtgttg gctcgacgct ggaaattgaa tccggcagta atgatccaat ggcggtcttt
    18721 ctgacgatta ccctgattgc gatgatccag cagcatgaaa gcagtgtcag ctggatgttc
    18781 gtggtcgata ttctgcagca atttggcctc ggaattgtca tcgggcttgg cggtggttat
    18841 ttactgctgc aaatgattaa tcgcatcgcc ctgcccgccg gattatatcc attgctggca
    18901 ttaagcggcg gtattttaat ttttgcttta accacggcgc tggaaggcag cggtattctg
    18961 gctgtttatc tgtgcggttt tctgctgggt aatcggccga ttcgcaaccg ctacggcatc
    19021 ctgcaaaatt tcgacggcct cgcctggctg gcgcaaatcg ccatgttcct ggtgctgggg
    19081 ctattggtta acccaagcga tctgctgcct attgccattc cggcgctcat tttgtccgca
    19141 tggatgattt tcttcgcccg tcctctttcg gtatttgccg gattgctacc gttccgtggc
    19201 ttcaatctgc gtgagcgcgt gtttatcagc tgggtaggat tacgcggcgc ggtgccgatt
    19261 attctggctg tgttcccgat gatggcgggg ctggagaatg cgcgactgtt ctttaatgtc
    19321 gccttctttg tggtcctggt ttcactgcta ttgcagggaa catcactctc gtgggcggcg
    19381 aaaaaagcca aagtggtcgt tccgccagtg ggacgtccgg tgtcacgcgt tggcctggat
    19441 attcatccgg aaaatccgtg ggagcagttt gtttatcaat tgagtgccga taaatggtgc
    19501 gtgggcgcgg cactgcgtga tttgcatatg ccaaaagaga cgcgtattgc ggcactgttt
    19561 cgtgataacc agttgcttca tcccaccggc agcacccgac tgcgcgaagg cgatgtgttg
    19621 tgtgtaattg gtcgggaacg cgatctcccg gcgctcggta aactgttcag ccagtcgccg
    19681 ccggtcgcgc tggatcaacg cttctttggt gacttcattc tcgaagccag cgccaaatat
    19741 gctgatgtgg cgctgatata tggcctggaa gacgggcgag aatatcgcga taagcagcaa
    19801 acgctgggtg aaatcgtcca gcagttgtta ggcgcagcac cggttgtcgg tgaccaggta
    19861 gagtttgccg ggatgatctg gacggtggcc gagaaagaag ataatgaagt gttgaagatt
    19921 ggtgttcggg tagcggagga agaagccgaa tcttaagttt aatgtgtttt gtaacgctgg
    19981 atacgacgct ggtcgcatcc agcgataaca agttacaccg tcacaaccgg gacgcgtaac
    20041 gccagcgcgc acatcaactc atagcctacc gttccggcag cggcggcgac atcatcaatt
    20101 ttgatctcct tgccccacag ctcaactggc gtaccaatac ccgcttgcgg gcaaggcgtt
    20161 aaatcgaccg ccagcatatc catcgagact gtccccaccg tcatggtgag aacgccatcc
    20221 actaaaacag gggtaccggt aggcgcgtgg cgcggataac cgtcggcgta tcctgcggcg
    20281 acaatgccga ttcgctgttc atcgcgcgca gtatagcgac cgccgtagcc cacacgctcg
    20341 cccgctttta gcgtctggac accaataatc tcactgctta gcgtcatcac cggacgtaat
    20401 ccggtattgg cgatatcacg ccactgaccg gacggcgaag cgccatacaa aataatacca
    20461 ggccgaaccc agtcaaaatg cgcttccggg tgccacagag tcgccgccga gttagacaac
    20521 gagcgtcgac actccagccc ctccgccgcc tggtcaacac gcgccatcgc gctggaaatt
    20581 ccatcaggat gttccgcttc ggcaaaatgc gacatcaggg tcatttcgcc aacattcgcc
    20641 atcgcccgca actgctgcca gacggtaagc acgcgatcgg gctggaagcc caaccgattc
    20701 attccactgt tcactttaag ataaatatcc aacggtgctt ttagccgcgc attttgcagt
    20761 gccttgagct gccagttgct atgtacgcag gtggtcaggc ggtgctggtc ataaatctcc
    20821 agatcctgag catggaaaaa tccttccagc atcaggatcg gtcctttcca gccgcgctca
    20881 cgtaacgtta ttgcctcttc cagattaagc aatgcaaagc catcggtggc cccgagcgcg
    20941 ctccagatac gctcaatacc gtgcccgtaa gcgttcgctt ttaccaccga ccagacgcgc
    21001 gcgtacggcg cggcctggcg gacaatggac agattctgtt ttaatgcctg cagatcgagg
    21061 ctggcctgta tcggacgggt catctcgttt ccttagctgt gtgcgccatg taaatggccc
    21121 gggcgtgatg gcgtaaatcc acggctgtag cgcgctacgc ttagatcttc atacgggatc
    21181 gctggcgtgc gccccgagag cagatcgctt aacaactgac cggaaccgca agccatcgtc
    21241 cagccgagcg tgccgtgacc ggtattcagc cacagatttt taaagcgtgt acgcccgaca
    21301 accggcgtgc cgtctggcgt catcgggcgc aggccagtcc agaaagtcgc ctgctcaaca
    21361 tgaccgccgc gtggatagag atcgcgaacc accatctcca gcgtttcacg acgcggttgc
    21421 aacagctcgg tgttaaaacc aacaatttcc gccattccgc cgacacgaat gcggttatcg
    21481 aaacgggtaa tggcgatttt gtaggtttca tcaagaatag tggatactgg cgcaccatct
    21541 tcctgcgcaa tgggaatggt cagcgagtag cctttcagcg gatacaccgg aatatcaaca
    21601 atgcctttga gcatcgccgt cgagtaagaa ccaaacgcca tcacatacgc atcggcctta
    21661 atcacttcat caccacactt cacgccgtag atttgctcgc cgtcgcaaag cagttggtca
    21721 acgggcgtat taaagcggaa tttaaccccc gcctgctccg ccatccgcgc cagattctgg
    21781 gtaaacagct ggcagtctcc ggtttcatca ttgggcagtt gcaggccgcc cgtcagtttg
    21841 tgcgccactt ctgccaacgc gggctccact tccgccaggc ggctggagtc cagcagctga
    21901 tacggtacgc cagcatcttc cagcacggcg atatcgcggg tcgcgttttc atactgttgt
    21961 tcggtacgga acagttgcag cgtcccaccc tgacgccctt catactgaat attggtttcg
    22021 gcgcgcaatg ctttcaggca atcacggctg tattccgcca gacgcaccat ccgccctttg
    22081 ttttccatgt agtggctggt gtcgcagtta cgtaacattt gccacatcca tttcaactgg
    22141 aactgcgtac cgtcgagacg aaccgccagc ggcgcatggc gctggaacat ccatttaatc
    22201 gcttttaaag gcacacctgg tgccgcccac ggtgcagcat atccggggga gatttgcccg
    22261 gcattcgcgg cactggtttc cagggcggcc cccggctccc gatcaatgac ggtgacctca
    22321 tgtcctgcct gatttaagta ccaggcgctg gcaacgccta ccacaccact tcccagtatg
    22381 acaactcgca tagccactcc gataacggta gatacagtaa aagaataata atccaattac
    22441 atcctgataa ctcagttgaa aatattattc aacatatggc ttttttatgg tgagctggct
    22501 cacatctccc tgactctgcg gcaatatggc gtctttagta tctcctgctg aacgcccttt
    22561 ttatccagca taaaattccg ttcagaagcg gattagtggc actctgataa taagaaatcg
    22621 caaagaaaaa aattctccgg cgacgcggtt tttaacaggt gttctatgct tgaaatgagg
    22681 tgctccacac agagagcgcc gacaacaatg aggtgcgcgt atggcgacga tcgattctat
    22741 gaataaggac accacacgtt tgagcgatgg acccgactgg acgttcgacc tgctggatgt
    22801 ttatctggca gagatagacc gggtggcgaa actctaccgg ctggatacct acccgcacca
    22861 gattgaagtg ataacctcag aacagatgat ggatgcctac tccagcgtcg gcatgccaat
    22921 taactatccg cactggtcat tcggtaaaaa gtttatcgag actgaacggc tgtataagca
    22981 cggtcagcaa ggactggcct atgaaatcgt cattaactct aacccgtgta tcgcttacct
    23041 gatggaagag aacaccatta ccatgcaagc gctggtgatg gctcatgcct gctatgggca
    23101 taactctttc ttcaaaaaca attacttatt ccgtagctgg accgacgcca gttcgattgt
    23161 cgattatctg atttttgccc gtaaatatat taccgagtgc gaagagcgtt atggcgttga
    23221 tgaagtagaa cggcttctgg actcgtgcca cgcgctgatg aactacggcg tggaccggta
    23281 caaacgcccg caaaaaatct cgctgcaaga agagaaagcc cggcagaaga gtcgcgaaga
    23341 gtatctgcaa agtcaggtca atatgctctg gcgtaccctg ccgaagcgcg aggaagagaa
    23401 aacggttgct gaagcgcgcc gctatccgtc cgaaccacaa gaaaacctgc tctattttat
    23461 ggagaaaaat gcgccactgc tggaatcatg gcagcgagaa atcctgcgta ttgtgcgtaa
    23521 ggtgagtcag tatttttatc cgcaaaaaca gactcaggtg atgaacgaag gctgggcgac
    23581 cttctggcac tacaccatcc ttaaccatct gtatgatgaa gggaaagtaa cggaacgttt
    23641 tatgctggag tttttgcaca gccacaccaa tgtggtcttc cagccgccct ataacagccc
    23701 gtggtacagc ggcatcaatc cgtatgccct cgggtttgcc atgttccagg atattaaacg
    23761 gatttgtcag tcgccaacgg aagaagacaa atactggttc ccggatatcg ccggttccga
    23821 ctggctggaa acgctgcatt tcgcgatgcg tgatttcaaa gatgagagtt ttatcagcca
    23881 gttcctgtca ccgaaagtga tgcgtgattt ccgcttcttc accgtgctgg atgatgatcg
    23941 gcataattat ctggagattt ccgctattca taatgaagaa ggttatcggg aaatccgtaa
    24001 ccggttatca tcgcaatata acttaagtaa tctggagccg aatattcaga tctggaacgt
    24061 ggatttgcgc ggcgaccgtt cgctgacgct gcgttacatt ccacataatc gcgcaccgct
    24121 ggatcggggg cgcaaagaag tgctgaagca tgtgcatcgc ctgtggggat ttgatgtgat
    24181 gctggaacag caaaacgaag acggcagcgt cgagttgctg gaacgttgcc cgccgagaat
    24241 gggaaatctg taaatagtcc tcctcgtttg aggaggactc atttttgccg gatgcggcgt
    24301 aaatgcctta tccggcctac aaagtatcgc aaattcaata cattgcaaaa aaaacacgta
    24361 ggcctgataa gcgtagcgca tcaggcaatt atacgtttgt catccgtctg gaaggattat
    24421 cgcccctgaa tggctaaatc acccggcaga tttttctgca tccggtgcca aatctcgcca
    24481 ctctcatgcc catagcgacg cactgtttca tatacctgat cgtgtgcgcc ttcactgcac
    24541 aacgccgaca gtttgtggta gaagcccagc gccagactgc gcgcttccgg attggcgaaa
    24601 tagtgacgac caatgcgcgt atacagccct ttcatcccgt taaggatcag accgtaaatc
    24661 gggttgccgg aagcaaacgc caggccgcgg aaaatgttgt aatccagctc ggcaaaggcg
    24721 tcggcgtgat cggccacttc attagcggtt gccagcactt cctgcgcttt atcgggatgt
    24781 tgacgaaacg cggtgcgaat aaaaatagtt gaaatattgg tacgcaccga cagcaaatta
    24841 tcaataagct gcggcacact ttcgtggtcc agtcgcgcca gtgtttcaag gatatttaaa
    24901 ccagaggttt cccagaaatt attcaccttc gtcggcttgc cgtgttgaat ggtcaaccag
    24961 ccatctcgtg ccagacgttg taacacttca cgtaacgtag tacgcgttac gccaattaat
    25021 tctgaaagtt cacgttctgc gggcaaaata gtcccgggag ggaagcggtt attccagata
    25081 ctttcaataa tgtactcttc cgcgaaaccc gccgggcttt gcgccttaat gaccatagtg
    25141 agatttccat aacacagcaa aacaaagttg gactcatcat accagagggc taactacgct
    25201 gatagaagac aaaaagaaga aaaaagggat caaggcttca ttttctgcat ctctttcccc
    25261 ttgactatca agacttgcct cactcctgtc tgaccgttaa gctttgcgtt taaacataaa
    25321 aacatcattg attatttcta aggtaaggga aaccatcatg gagatctcct ggggccgcgc
    25381 actatggcgc aattttttgg gccagtcccc cgactggtac aaactcgccc tcatcatttt
    25441 cttaatcgta aatccgttaa ttttcctcat cagccctttc gtcgctggct ggttgctggt
    25501 agcggaattt attttcactc tggctatggc cctgaaatgc tacccgctgc tccccggtgg
    25561 tttgttggct atcgaagcag tattcatcgg catgaccagc gcggaacatg ttcgtgaaga
    25621 agtggcggca aatcttgaag tcttgctgtt actgatgttt atggtggcgg gtatctattt
    25681 tatgaaacag ctgttgttgt tcatatttac ccgtttgctg ttaagcattc gctccaaaat
    25741 gctactgtcg ctctcttttt gcgtggcggc ggcgttcctc tccgcattcc tcgatgcctt
    25801 aaccgtcgtg gcggtggtga tcagcgttgc agtcggtttt tatggtattt atcatcgcgt
    25861 agcctcttcc cgtgccgaag ataccgacct gcaagacgat agtcatatcg acaagcatta
    25921 caaagtggtt ctggaacagt tccgtggctt tctgcgtagt ctgatgatgc atgccggtgt
    25981 cggcaccgca ttaggcggcg tgatgaccat ggtgggcgaa ccacagaacc tgatcatcgc
    26041 taaagcggct ggctggcatt ttggcgattt cttcctgcgc atgtcgccgg tgaccgtccc
    26101 ggttctgatt tgtggcctgt taacctgcct gctggtagag aagctgcgtt ggtttggcta
    26161 cggtgaaacg ctgccggaga aagtccgcga agtgctgcaa cagtttgacg atcaaagccg
    26221 tctccagcgt acacgtcagg acaaaattcg tctgattgtg caggcgatta tcggcgtctg
    26281 gctggtgact gcgctggcac tgcatctggc ggaagttggt ttgattggtt tgtcagtcat
    26341 tattctggcg acatcattga ccggtgttac cgatgaacat gctatcggta aagccttcac
    26401 cgaatctctg ccattcaccg cactgttgac ggtgtttttc tcagtagtcg cggtgattat
    26461 cgaccaacaa ctgttttcgc cgattattca gtttgtgttg caggcatctg aacatgctca
    26521 gctgtcgttg ttctatattt tcaacggtct gctgtcatcc atttcggata acgtcttcgt
    26581 ggggacgatt tatatcaacg aagcgaaagc ggcaatggaa agtggcgcta tcacgttgaa
    26641 gcaatatgag ctgctggcgg tcgccattaa taccggtacc aatctgccct ccgtcgctac
    26701 gccgaacggt caggctgcgt tcctgttcct gctgacctct gcactcgcgc cattgattcg
    26761 tctctcttat ggccgcatgg tgtggatggc cctgccttac accctcgtcc tgacactcgt
    26821 cgggctgctc tgcgtcgagt ttacgcttgc tcctgtaacc gaatggttta tgcaaatggg
    26881 ctggatagca acgctttgat aacaacttac cgggcatata aatgcccggt ttgccttttc
    26941 gcccgataat tgtccaattg cgtcctattt ttgctgcctc ctggtggcgc agcgaatgaa
    27001 ttggtttaaa ctgcgcactc tatgcatatt gcagggaaat gattatgttg cgatttttga
    27061 accaatgttc acaaggccgg ggcgcgtggc tgttgatggc gtttactgct ctggcactgg
    27121 aactgacggc gctgttgttc cagcatgtga tgttactgaa accttgcgtg ctctgtattt
    27181 atgaacgctg cgcgttattc ggcgttctgg gtgctgcgct gattggcgcg atcgccccga
    27241 aaactccgct gcgttatgta gcgatggtta tctggttgta tagtgcgttc cgcggtgtgc
    27301 agttaactta cgagcacacc atgcttcagc tctatccttc gccgtttgcg acctgtgatt
    27361 ttatggttcg tttcccggaa tggctgccgc tggataaatg ggtgccgcaa gtgtttgtcg
    27421 cctctggcga ttgcgccgag cgtcagtggg aatttttagg tctggaaatg ccgcagtggc
    27481 tgctcggtat ttttatcgct tacctgattg tcgcggtgct ggtggtgatt tcccagccat
    27541 ttaaagcgaa aaaacgtgac ctgttcggtc gctaatccat tcggcgctcc tgcgggagcg
    27601 ctttttcctg ccgctatgtt tatttgaccc gcagtaaatc agaacttcgc gttgtataac
    27661 atggtgaaag catggcacgc ttcatctgcc attgttgctg gatcccctgc ccggcaaaat
    27721 agagtgttcc tctgccctct ttagcattca gcgtatccat taccgccatc aattgctcac
    27781 tcccggggcg cggtgcgttg tcatcgaata aattgagctg cgcgactccc tgactgaaga
    27841 aatcccccag catcacgccc gctttttggt agcgatggcc cgcttgccag atggcatcca
    27901 gagatcgcgt agcggcgtta atgatatccc tgctgtcctg agtgggcgtc agcagtttta
    27961 ccgatgcgct attgccgtaa taaggttcat tgaacgcaaa tggtgacgtc ttaataaacg
    28021 tggagataaa ccgacaatat tgatgctcgc tgcgaagttt ttccgccgcc cgggcagcgt
    28081 aactacaaat ggcctgccgc atcgacggat aatccgtgat gcgttcacca aacgagcggg
    28141 aacagataat ttcctgcttc gtcggtgcaa actcttccag ttgcaaacag ggttcaccgc
    28201 gcagttcacg caccgttctt tcgagcacga cattaaaatg tttacggata aaccggatat
    28261 ctgaatccgc caaatcgaga acggttttga tccccatcgc gtccagtttt ttgctgatcc
    28321 gccgtccaat cccccagact tcatccacgg gaagagcaga cattaattta cgctggcgtt
    28381 ccagatttga taaatccacc accccgcccg tctgccgctg ccattttttt gccgcatgat
    28441 tggcaagctt agccagcgtt ttggtctggg cgatccccac accaacagta agatgggtac
    28501 gttgtagcac cgttgcgcga atttctctgc caaaatcagt cagatcgcga caattatgca
    28561 cacctgtcag atcgcagaat gcctcatcaa tactgtaaat ctcgacgcgg ggcgatagtt
    28621 cttccagcgt cgacatcacc cgattactca tgtctgcgta aagctcataa ttgctgctaa
    28681 agcaaaccac gccacagcga cgaaacagat ctttttgttt gaaccaggga tcgcccattt
    28741 taacgcccag cgcctttgcc tcagcgtttc gggcgataac gcaaccgtca ttattcgata
    28801 gcacaaccac cggtttaccc cataaatcag ggcgaaacac cgtctcacag ctggcataaa
    28861 acgcgtttac atcacagagg gcaaacatca gcgcatcgcc ttaacgacgt ggatcaccac
    28921 accaaagaca tccagcgtat cttcgctact gatggtaatg ggcgagtacg cactgttcat
    28981 aggagtaagc tgtaccgtcg ggcgtagttg caattttttc accgtaaact caccgtcaac
    29041 agcagcgatg acaatatcac catggctggc ggtaatagcg ctatcgacaa tcagtaaatc
    29101 accgtcacta atcccaccat caatcataga atcaccactt gctttgacga aataagtcgc
    29161 gctgggatgc tggatcaaca gttgattcag atcgatgcgc tgttcaacgt aatctgcggc
    29221 cggtgaagga aagccacact gaacaagatc gctaaatagc ggaaaagtca caatttcgcg
    29281 gagatccgca ggcttgataa acaacataat aatctgcctg aagctatact gtttttatat
    29341 acagtagtct gttcttgtca gcagatcaat actgattcag gctatcaata tttgtcgctg
    29401 catagactgc tgatttttcg ttctcttatc ctgtgctcac gcggccttct tgcgacagca
    29461 tccatccagc agaaaggaaa aatatcacct ggtttaaaat agaatcgaag cattcgccca
    29521 gaaaggcatt ctcattaagg tactggatga cggcatcaag aaactgtgtt ctattgctgc
    29581 gggcgtagtt gaagggaaac tgattccaga attgaagaac aagttaaaat ctgtgcagaa
    29641 tttctttacc accctgtcta acacggttaa acaagcgaat aaagatatcg atgccgccaa
    29701 attgaaatta accaccgaaa tagccgccat cggggagata aaaacggaaa ctgaaacaac
    29761 cagattctac gttgattatg atgatttaat actttcttta ctaaaagaag cggcaaaaaa
    29821 aataattaac acctgtaatg agtatcagaa aagacacggt aagaagacac tctttgaggt
    29881 atctgaagtc tgataagcga ttattctctc tatgtactca aggtataaag tttatcacat
    29941 ttaaaaatac agacgttttt taaagattga gagttacctg ttgataataa ggtatcgagt
    30001 aactctcatt atgacagagc gctttcaaaa acagggctac gaaatggcga gcatccagac
    30061 gtgtattaag aaagccccca tcaacatcta ttaaatttat cactgcgccc agtcaggttt
    30121 atttaagata tgatcctgcc agtcaatcac ttctgattct ttcactgcga tatgacgcac
    30181 agagatacgt tcaccatgca ttgccgcttt cgaaccagta agtagcggat gccacgcagg
    30241 taaaccttta ccttccgcca gcaaacgata agcacaggtc attggcagcc attcgaatgt
    30301 tggcagattt tcacgggtta atttaatgca gtcgggttca aactcgaaac gacgttcgta
    30361 gttccgacat tgacaggttt taatattgag ctggcgacag gcgacgttag tgaagtagat
    30421 ttcgtcggtg tcttcatcca tcagtttatg caggcaacac tgaccgcaac catcacacaa
    30481 cgactcccat tccgcatcgc tcatttcgtc cagggtttta ctttgccaga aaggtacatc
    30541 gctcatcagg tgttccgcca ttacgttaaa acgcacctta taaccagtct ggcacagcga
    30601 tgcaagtttt gccgccgctt tcaggcggca aaaagtatta caaaacgcga gttgtcaaag
    30661 aatgcccatc gaaagtgact gtcagctcat caccactttg caacgggccg acgccatcag
    30721 gcgtgcctgt cagcacaacg tcaccggcct tgagggtaaa gaacttgctc atataagcga
    30781 tcagcggaac gattttatgg atcatgtccg cagtcgtacc ttgctggcgt tgttcgccgt
    30841 ttacgctcag gcccagcgtt gtattttgcg gatcgccggt gaattccgcc gcgggaataa
    30901 acccggaaag cggacaagag ttatcaaacg ctttggcctt ttcccacggc tgcccggctt
    30961 tcttcatttt tccctgaaca tcacgcaatg tcagatcgag cgccacgccg taaccggcaa
    31021 tggctttacg gacatgctct tccgtagcct gacgcagcgt cgcgccaatc aataccgcca
    31081 gttcgacttc atgatgaact gaaccgaaat cggatgggat cgccaatggc tgccgcagat
    31141 cgcacagtgc cgtttctggt ttaataaaca gcactggctc ttcgggcact gcgctgccca
    31201 tctctttaat atgtttggca tagttactac caacacagac tactttactc accggataat
    31261 ccagcagcgc accttgccag ttgtgatgtt gatacataac gttcccctca ccttatcgtg
    31321 gatatgaaaa cccgcgccga caaagataat gccaccgccg gatatcggtt atttgttcgt
    31381 gtcgtctgtt ttctgcccca tgacggaaag atgttgcttc agcaaatctt cgggtggtgg
    31441 cggtaactgc aaatagtaac cttgctcggt taatgcctgt ttaacctttt caatatcggc
    31501 attgaccagt ttcttccgcc catccagcgg cagaatcatc gctaactgag gctgaccaaa
    31561 acctttcatc agttcctcag gtacacgcga aaaatcgtct tttttttcga catataaata
    31621 ggtctggtca cgcttgctgc ttcgataaat cacacaaaac atacttttac tctttaagat
    31681 gccaggtttt ggcataagct aacatcaatg aaacccgcgc cacaagccag ccagctgtag
    31741 atttctgcgg tttgttcagt ccatgcgccc ttaataagtg atcccccggc aaaaatgccg
    31801 cgccgggatt taaccgaacg caggaaagcc actggaattc tcccgtaagg gattgttata
    31861 ttataacagt tcatcgtact cattctgaac aggagactac ctatgaaaat caagggcatc
    31921 agtaaggctg tattattgct tgcactgcta acctccacga gctttgctgc gggtaagaat
    31981 gtcaatgttg agttcagaaa aggccatagc agcgctcagt actccggcga aataaaggga
    32041 tacgattacg atacatatac cttctatgcc aaaaaaggtc agaaagtgca cgtgagtatc
    32101 tctaatgaag gcgctgatac ttacctcttc gggccaggaa tcgatgattc tgttgattta
    32161 tccagatatt ccccagaact cgatagtcac ggacaatact cactccctgc ttctggcaaa
    32221 tatgagctgc gagtgctgca aactcgaaac gacgcccgta aaaacaaaac taaaaaatat
    32281 aacgtcgata ttcaaattaa gtaaatgtca accgacgggg actctccccg tcataaacgt
    32341 taacgcccca ctcctctctg tcgctcaaga ggttaatgac gaatagaaca taatgtaaac
    32401 aagacatcac tcacgacaca taaataacat ctttgtggtg gtttgattga tttttccact
    32461 ccgcttacca tccacaccaa aataacgatc atcacgacat aatttttctt taacatcgca
    32521 aaatacaccg ttagcgaatg taaattcgct gggatcgtaa ccttgtactg attgtaagtt
    32581 ttcagcggct ttttcgccga gatatttcct tgttaactct ggggaaatgc catctgagtt
    32641 aacacaaata tacgggtcgc aaacaacgcc gcacgccgga gattgaagtc cgccagcgtc
    32701 atttccagac gccattgatg agaacatcat ccccacacct gacaatccga tataaaaaat
    32761 acgcatcata ttcatagcac atcctgataa tccaaccgta caacacttat gctcatttca
    32821 tcatcaatcc gtaatcaaaa ccgctgacct ggacgagtta cgatgacctg acagaagtat
    32881 cagacgtctt ctgcggtttc tatgatagag caattctgtg gcggatatta agataacggg
    32941 tatctgttct ttaatgatgc tgcaaaaaat ggaaagttaa gaaagcctgg cgtggtgaaa
    33001 aaatacactc gtctcaatac tggatattta aacaggtctg aagatgctgt cacgattagg
    33061 gtgcggaaaa aggacgcgta aataagaaca atcaaaagta cgacggcaat gggttgattg
    33121 acaagggtat tttttaagct atgaatcagc gccatttatc acagaataga cttttactct
    33181 gaataaatgg gagggtgact tgcctcaata taatccagac tataacatgc cttatagtct
    33241 tcggaacatc atcgcgcgct ggcgatgatt aatagctaat tgagtaaggc caggatgtca
    33301 aacacgccaa tcgagcttaa aggcagtagc ttcactttat ctgtggttca tctgcatgag
    33361 gcagaaccta aggttatcca tcaggcgctg gaagacaaaa tcgctcaggc ccccgcattt
    33421 ttaaaacatg cccccgttgt actcaacatc agtgcactgg aagccccggt aaactggtcg
    33481 gcgatgcata aggcggtttc ggcaaccggt ttgcgggtta ttggcgtaag tggctgcaaa
    33541 gatgcgcaac ttaaagccga aattgaaaag atggggctgc ctatcctgac ggaaggaaag
    33601 gaaaaagcgc cgcgtccagc tcccgcaccg caggctccag cgcaaaatac aacgccggtc
    33661 acaaaaacgc gtttaattga taccccagtg cgttccggtc agcgtattta tgctccacaa
    33721 tgtgatctga ttgttacaag ccacgttagc gctggggccg aattgattgc cgatggtaat
    33781 attcacgtct atggcatgat gcgcggtcgt gcgctggcag gggcaagtgg tgaccgggaa
    33841 acgcaaatat tttgtacgaa cctgatggcg gaactagtgt ccatcgcagg tgaatactgg
    33901 ctgagtgatc aaatcccagc agaattttat ggcaaagcgg cacgactgca gttagtcgaa
    33961 aacgctttga ccgttcaacc gttaaattga tcccttttta acaaggaatt tctatggcac
    34021 gcattattgt tgttacttcg ggcaaagggg gtgttggtaa gacaacctcc agcgcggcca
    34081 tcgccactgg tttggcccag aagggaaaga aaactgtcgt gatagatttt gatatcggcc
    34141 tgcgtaatct cgacctgatt atgggttgtg aacgccgggt cgtttacgat ttcgtcaacg
    34201 tcattcaggg cgatgcaacg ctaaaccagg cgttaattaa agataaacgt actgaaaatc
    34261 tctatattct tccggcatcg caaactcgcg ataaagatgc cctcactcgt gaaggggtcg
    34321 ccaaagttct tgatgatctg aaagcgatgg attttgaatt tatcgtttgt gactccccgg
    34381 cagggattga aaccggtgcg ttaatggcac tctattttgc agacgaagcc attattacca
    34441 ccaacccgga agtctcctca gtacgcgact ctgaccgtat tttaggcatt ctggcgtcga
    34501 aatcacgccg cgcagaaaat ggcgaagagc cgattaaaga gcacctactg ttaacgcgct
    34561 ataacccagg ccgcgtaagc agaggtgaca tgctgagcat ggaagatgtg ctggagatcc
    34621 tgcgcatcaa actcgtcggc gtgatcccag aggatcaatc agtattgcgc gcctctaacc
    34681 agggtgaacc ggtcattctc gacattaacg ccgatgcggg taaagcctac gcagataccg
    34741 tagaacgtct gttgggagaa gaacgtcctt tccgcttcat tgaagaagag aagaaaggct
    34801 tcctcaaacg cttgttcgga ggataaatta tggcattact cgatttcttt ctctcgcgga
    34861 agaaaaacac agccaacatt gcaaaagaac ggttgcagat tattgttgct gaacgccgtc
    34921 gcagcgatgc agaaccgcat tatctgccgc agttgcgtaa agatattctt gaggtcattt
    34981 gtaaatatgt acaaattgat cctgagatgg taaccgtaca gcttgagcaa aaagatggcg
    35041 atatttctat tcttgagctg aacgtgacct taccggaagc agaagagctg aaataagccc
    35101 cctgtaaaag cgcatttatc ttcaaggcag agtcatctct gccttgagtt tttcatccct
    35161 ctcatccacg ttgtggtaaa gcggcgagta ttcttgctga tactactcat tgctatttcc
    35221 ccctccccct ttacacctta aggctgttcc cacgcacaga acaaaatgtt tacgcctggt
    35281 tgagtaaata accttatttt tagagtgggt atcctgggag atgataaccc cgcaatttca
    35341 ttattcattg ttctcccata ggatgaggtt tcccgccgta actggcgggc tttttttgcc
    35401 ccagaatttt cccttttagc acccggtaaa cgaaaacagc gccgaagcgc agtttaatcg
    35461 ttgatggtca gaacgaccag ttcacaccgg ccaccgcatt ccacggggac tccacaccgg
    35521 caccacggct ataccccccc ccgagatgcc cgcttaacgt actgctgaat gaggctttaa
    35581 tacctgcctg gtatattcca cgtctgcccg acaaatcatt gacaaaatta ccgtcactat
    35641 tcactttcac tcgattatca tcgacaaatt atttgcgcac agccgccttc agccagggtt
    35701 caatttccat accgttcccc agacgcatgt tgtaactcag cgttgcgccc agttcacgat
    35761 atatactgcg ggtatcgact gatttcgatt ccatgcattg gataaatgat agtagcgtct
    35821 aagtgacctt agctctgctg caacatcagc ccgcaggcac cagaccaggg gattcatcct
    35881 gaagagacag cgcaaatgta ttgtgttcac cgctcatcaa agacatcatg atgaaatgat
    35941 gatatcccgt ataagaaaga ggtatttttt aaacgcagtg cgctgaagtg ttgttggata
    36001 aaaaatcact cttttctgat aatactgtcg tacgcttatt tttcgacaat gaggccgtcc
    36061 acaaaataaa atgactaatg taatcgttgc taatgaatat cttctgctca cataacaatc
    36121 acacaataat att
//
LOCUS       DAZFJL010000030        35690 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFJL010000030 DAZFJL010000000
VERSION     DAZFJL010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.030"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            70..1209
                     /gene="arnB"
                     /locus_tag="ACHJ8C_001519"
     CDS             70..1209
                     /gene="arnB"
                     /locus_tag="ACHJ8C_001519"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO5551398.1"
                     /translation="MSGFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIEAAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     HTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITVLQQLAGQ"
     gene            1213..2181
                     /gene="arnC"
                     /locus_tag="ACHJ8C_001520"
     CDS             1213..2181
                     /gene="arnC"
                     /locus_tag="ACHJ8C_001520"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO5551399.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTAACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAEGSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2181..4163
                     /gene="arnA"
                     /locus_tag="ACHJ8C_001521"
     CDS             2181..4163
                     /gene="arnA"
                     /locus_tag="ACHJ8C_001521"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO5551400.1"
                     /translation="MKTVVFAYHDMGCLGIEALLSTGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDKILQLAPRGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NTVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDSITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHYFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLNWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4160..5050
                     /gene="arnD"
                     /locus_tag="ACHJ8C_001522"
     CDS             4160..5050
                     /gene="arnD"
                     /locus_tag="ACHJ8C_001522"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO5551401.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGTPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRIQRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGTLPLGQVV
                     RGNIAGREGWLGCQQMAGSH"
     gene            5050..6702
                     /gene="arnT"
                     /locus_tag="ACHJ8C_001523"
     CDS             5050..6702
                     /gene="arnT"
                     /locus_tag="ACHJ8C_001523"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO5551402.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYLPVVIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAILMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADVIDRQERLVLIQYRPK"
     gene            6699..7034
                     /gene="arnE"
                     /locus_tag="ACHJ8C_001524"
     CDS             6699..7034
                     /gene="arnE"
                     /locus_tag="ACHJ8C_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO5551403.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFATVNKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSLRHWCGVAFIIG
                     GIVILGSTV"
     gene            7034..7420
                     /gene="arnF"
                     /locus_tag="ACHJ8C_001525"
     CDS             7034..7420
                     /gene="arnF"
                     /locus_tag="ACHJ8C_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO5551404.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7414..7680)
                     /gene="pmrD"
                     /locus_tag="ACHJ8C_001526"
     CDS             complement(7414..7680)
                     /gene="pmrD"
                     /locus_tag="ACHJ8C_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO5551405.1"
                     /translation="MEWLVKKSCCNKQDNRHVIMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSANCYSPDEWERQCKAAGKTQ"
     gene            complement(7790..9145)
                     /gene="menE"
                     /locus_tag="ACHJ8C_001527"
     CDS             complement(7790..9145)
                     /gene="menE"
                     /locus_tag="ACHJ8C_001527"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO5551406.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSF
                     AVQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPEGENTFPALASLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNGQRVPLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQSVDLDEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9142..10104)
                     /gene="menC"
                     /locus_tag="ACHJ8C_001528"
     CDS             complement(9142..10104)
                     /gene="menC"
                     /locus_tag="ACHJ8C_001528"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO5551407.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQESWEEAQSVLLAWVNNWLAGDCEIPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLDKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDVDALERLL"
     gene            complement(10104..10961)
                     /gene="menB"
                     /locus_tag="ACHJ8C_001529"
     CDS             complement(10104..10961)
                     /gene="menB"
                     /locus_tag="ACHJ8C_001529"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO5551408.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10976..11734)
                     /gene="menH"
                     /locus_tag="ACHJ8C_001530"
     CDS             complement(10976..11734)
                     /gene="menH"
                     /locus_tag="ACHJ8C_001530"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO5551409.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSATISVDGFDDVTDLLCKTLVSYNILNFWLVGYSLGGRVAMMAACQELAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11731..13401)
                     /gene="menD"
                     /locus_tag="ACHJ8C_001531"
     CDS             complement(11731..13401)
                     /gene="menD"
                     /locus_tag="ACHJ8C_001531"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO5551410.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGISWQQRLGDWWQDDKPWLREAPRRESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     AMALLRQVSAPLVLIVVNNNGGQIFSLLPTPKNERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13490..14785)
                     /gene="menF"
                     /locus_tag="ACHJ8C_001532"
     CDS             complement(13490..14785)
                     /gene="menF"
                     /locus_tag="ACHJ8C_001532"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO5551411.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQVYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNIVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14864..15169)
                     /gene="elaB"
                     /locus_tag="ACHJ8C_001533"
     CDS             complement(14864..15169)
                     /gene="elaB"
                     /locus_tag="ACHJ8C_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO5551412.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15224..15685)
                     /gene="elaA"
                     /locus_tag="ACHJ8C_001534"
     CDS             complement(15224..15685)
                     /gene="elaA"
                     /locus_tag="ACHJ8C_001534"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5551413.1"
                     /translation="MIDWQDLHHSDLSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     EGENRHILGWHNGTLVAYARILKSDDELQPVVIGRVIVSEALRGEKIGQQLMSKALES
                     CTRRWPEKPIYLGAQAHLQNFYGQFGFIPVTDIYEEDGIPHIGMAREVIQA"
     gene            15750..16667
                     /gene="rbn"
                     /locus_tag="ACHJ8C_001535"
     CDS             15750..16667
                     /gene="rbn"
                     /locus_tag="ACHJ8C_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO5551414.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPHGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEIFDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTIMLDDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALELAKGVDVMVHEATLDMAMEAKANSRGHSSTRQAAALAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFAVFNV"
     gene            16855..16971
                     /locus_tag="ACHJ8C_001536"
                     /pseudo
     CDS             16855..16971
                     /locus_tag="ACHJ8C_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
     gene            17023..17994
                     /gene="yfbL"
                     /locus_tag="ACHJ8C_001537"
     CDS             17023..17994
                     /gene="yfbL"
                     /locus_tag="ACHJ8C_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFO5551415.1"
                     /translation="MKKMTSLCFIPLLLSLTFIIFYQPWVRALPPTPRHASPEQLEKT
                     VRYLTQTVHPRSADNIDNLNRSAEYIKEAFISRGARITAQDIPITGGPYKNIVANYGP
                     ADGPLIIIGAHYDSVSSYENDQLTYTPGADDNASGVAGLLELARLLQQQTPGIGVQLV
                     AYASEEPPFFRSDEMGSAVHAASLERPVKLMIALEMIGYYDSTPGSQDYPSPAMSWLY
                     PDRGDFIAVVGRMQDINAVRQVKAALLSSRDLSVYSMNAPGFIPGIDFSDHLNYWQHD
                     IPAVMITDTAFYRNKQYHLPGDIADRLNYQKMAQVVDGVTTLLYNSK"
     gene            18097..18600
                     /gene="yfbM"
                     /locus_tag="ACHJ8C_001538"
     CDS             18097..18600
                     /gene="yfbM"
                     /locus_tag="ACHJ8C_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO5551416.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTKKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(18667..20124)
                     /gene="nuoN"
                     /locus_tag="ACHJ8C_001539"
     CDS             complement(18667..20124)
                     /gene="nuoN"
                     /locus_tag="ACHJ8C_001539"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO5551417.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLVGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(20131..21660)
                     /gene="nuoM"
                     /locus_tag="ACHJ8C_001540"
     CDS             complement(20131..21660)
                     /gene="nuoM"
                     /locus_tag="ACHJ8C_001540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO5551418.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALVTMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILVLVFVHYNATGVWTFNYEELLNTPMSNG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(21824..23665)
                     /gene="nuoL"
                     /locus_tag="ACHJ8C_001541"
     CDS             complement(21824..23665)
                     /gene="nuoL"
                     /locus_tag="ACHJ8C_001541"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO5551419.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(23662..23964)
                     /gene="nuoK"
                     /locus_tag="ACHJ8C_001542"
     CDS             complement(23662..23964)
                     /gene="nuoK"
                     /locus_tag="ACHJ8C_001542"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO5551420.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(23961..24515)
                     /gene="nuoJ"
                     /locus_tag="ACHJ8C_001543"
     CDS             complement(23961..24515)
                     /gene="nuoJ"
                     /locus_tag="ACHJ8C_001543"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO5551421.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPVSAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(24527..25069)
                     /gene="nuoI"
                     /locus_tag="ACHJ8C_001544"
     CDS             complement(24527..25069)
                     /gene="nuoI"
                     /locus_tag="ACHJ8C_001544"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO5551422.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(25084..26061)
                     /gene="nuoH"
                     /locus_tag="ACHJ8C_001545"
     CDS             complement(25084..26061)
                     /gene="nuoH"
                     /locus_tag="ACHJ8C_001545"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO5551423.1"
                     /translation="MSWISPELIEILLTVLKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(26058..28784)
                     /gene="nuoG"
                     /locus_tag="ACHJ8C_001546"
     CDS             complement(26058..28784)
                     /gene="nuoG"
                     /locus_tag="ACHJ8C_001546"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO5551424.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNMVTLPVEISEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(28837..30174)
                     /gene="nuoF"
                     /locus_tag="ACHJ8C_001547"
     CDS             complement(28837..30174)
                     /gene="nuoF"
                     /locus_tag="ACHJ8C_001547"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO5551425.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDSGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(30171..30671)
                     /gene="nuoE"
                     /locus_tag="ACHJ8C_001548"
     CDS             complement(30171..30671)
                     /gene="nuoE"
                     /locus_tag="ACHJ8C_001548"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO5551426.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(30674..32476)
                     /gene="nuoC"
                     /locus_tag="ACHJ8C_001549"
     CDS             complement(30674..32476)
                     /gene="nuoC"
                     /locus_tag="ACHJ8C_001549"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO5551427.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMVEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(32570..33232)
                     /gene="nuoB"
                     /locus_tag="ACHJ8C_001550"
     CDS             complement(32570..33232)
                     /gene="nuoB"
                     /locus_tag="ACHJ8C_001550"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO5551428.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(33248..33691)
                     /gene="nuoA"
                     /locus_tag="ACHJ8C_001551"
     CDS             complement(33248..33691)
                     /gene="nuoA"
                     /locus_tag="ACHJ8C_001551"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO5551429.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(34321..35259)
                     /gene="lrhA"
                     /locus_tag="ACHJ8C_001552"
     CDS             complement(34321..35259)
                     /gene="lrhA"
                     /locus_tag="ACHJ8C_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO5551430.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         8629 a         9757 c         9143 g         8161 t
ORIGIN      
        1 gcaaaatata cacaacactc tttatagcaa atataagtgg acaggtattc aatgacggat
       61 ggaaaaacaa tgtcaggatt tttgcctttt tcgcgaccag caatgggcgt agaggaactc
      121 gctgcagtta aagaggttct cgaatccggt tggatcacaa ccggtccgaa gaatcaggcg
      181 cttgaacaag ctttttgcca gttgacggga aatcagcatg ccatcgcggt cagttcagcc
      241 accgccggaa tgcatatcac gctaatggcg ttggaaattg gcaagggcga tgaagtgatt
      301 acgccttccc tgacctgggt ttcaaccctc aatatgattt ccttgctggg tgcaacgccg
      361 gtaatggtgg atgtcgaccg cgatacgctg atggtcacgc ctgaagcgat cgaagcagcc
      421 attacgccac gcactaaagc catcattccg gtgcattatg ccggtgcgcc agcagatatt
      481 gacgccattc gcgccattgg cgaacgttac ggcatcgcag ttatcgaaga tgctgcccat
      541 gccgtcggta cgtattacaa agggcgacat attggcgcaa aaggtactgc tattttttcg
      601 tttcacgcca tcaaaaatat tacctgtgcg gaaggtggcc tgattgttac tgataatgaa
      661 aatcttgccc gccagctgcg gatgctgaaa tttcacggtc tgggtgtcga tgcctatgac
      721 agacatacct ggggccgtgc accgcaggct gaagtcttaa cgccgggcta taagtacaat
      781 ctgaccgata ttaacgccgc gattgccctg acacagttag tcaaattaga gcacctcaac
      841 acccgtcggc gcgaaattgc ccagcaatat cagcaagcac tggcagctct cccctttcag
      901 ccattaagcc ttcccgcctg gccgcacgtt cacgcctggc atctgtttat tattcgtgtc
      961 gatgaacaac gttgtggtat cagtcgcgat gcgttgatgg aagcgttaaa agaaagaggc
     1021 attggtaccg ggttacattt ccgcgccgct cacacacaaa aatattatcg cgagcgtttt
     1081 cccacgctgt cgttaccgaa taccgaatgg aatagcgaac gcatctgttc gttgccgctg
     1141 ttcccggata tgaccaccgc cgatgccgac cgcgtcatca cagtccttca gcaactcgca
     1201 ggacaataag ccatgtttga aatccaccct gttaagaaag tctcggtggt tattcccgtt
     1261 tataacgagc aggaaagctt accggaatta atcaggcgca ccaccgcagc ctgtgaatca
     1321 ctggggaaag agtatgaaat cctgctgatt gatgacggca gtagcgataa ttccgcgcat
     1381 atgctggtcg aagcctcaca agcggagggc agccatattg tgtctatctt gcttaaccgc
     1441 aattacgggc aacattcagc gattatggcg ggattcagtc acgtcaccgg cgacttaatt
     1501 attaccctcg atgccgatct ccagaatccg ccagaggaga tcccccgtct ggtggcaaaa
     1561 gccgatgaag gttacgacgt ggtagggact gtacgccaga accgccagga cagctggttt
     1621 cgtaaaaccg cttcgaagat gattaaccgg cttattcagc gcaccacagg caaagcgatg
     1681 ggtgactatg gttgtatgct gcgcgcctat cgccgtcata ttgtcgatgc gatgttgcac
     1741 tgccatgaac gcagcacctt tatcccgatt ctggcgaata tcttcgcccg ccgtgccatt
     1801 gaaattccag tgcaccatgc cgagcgtgag tttggtgaat ccaaatacag ctttatgcgc
     1861 ctgattaatt tgatgtacga cctggtgacc tgccttacca caacgccgct acgtatgttg
     1921 agtttgctcg gcagcattat tgcgattggc ggttttagca ttgcggtgct gctggtgatt
     1981 ttacgcctga ccttcggacc acaatgggcg gcagaaggcg tctttatgct atttgccgtg
     2041 ctgtttactt ttattggcgc tcagtttatc ggcatgggat tgctcggtga atatatcggc
     2101 aggatctaca ccgatgtccg cgcccgcccc cgctattttg ttcagcaagt tatccgtcca
     2161 tccagcaagg aaaatgaata atgaaaaccg tcgtttttgc ctaccacgat atgggatgcc
     2221 tcggtattga agccctgctg tctaccggtt acgaaattag cgccattttt acccatactg
     2281 ataatcccgg tgaaaaagcc ttttatggtt cggttgcccg actggcagcg gaaagaggca
     2341 ttccagttta tgcgccggat aacgttaatc atccgctgtg ggtggaacgc attgcccaac
     2401 tatcaccaga ggtgattttc tctttttatt atcgccatct tatttgcgat aagattttgc
     2461 agctcgctcc gagaggtgca tttaatctgc atggttcgct gttaccaaaa tatcgtggtc
     2521 gcgcgccgct gaactgggtg ctggtgaacg gtgaaacgga aactggcgtt acattgcacc
     2581 gaatggtgaa acgtgccgat gccggggcca ttgtggcaca actgcgcgtt gccattgcgc
     2641 cagacgatat cgccattacg ctgcatcata aattgtgcca tgccgcgcgc cagctactgg
     2701 aacagacatt acccgccatt aaacacggta atattctgga aatcgcccag cgcgaaaacg
     2761 aagccacctg ttttggtcgc agaaccccgg atgacagttt cctcgaatgg cataagccag
     2821 catccgtact gcacaacacg gtgcgtgccg ttgccgatcc gtggccgggt gccttcagct
     2881 atgttggcaa tcagaaattc accgtctggt cgtcgcgtgt tcatcctcat gccagcaaag
     2941 cacagccggg gagcgtgatt tctgttgcac cactgctgat tgcctgtggc gatggcgcgc
     3001 tggaaatcgt cactggacag gcgggcgaca gcattactat gcagggctcg caattagcgc
     3061 agacgctggg cctggtgcaa ggttcacgct tgaatagcca gcctgcctgt gccgcccgac
     3121 gccgtacccg ggtactcatc ctcggggtga atggctttat tggcaaccat ctgacagaac
     3181 gcctgctgcg cgaagatcat tatgaagttt acggtctgga tattggcagc gatgcgataa
     3241 gccgttttct gaatcatccg cattttcact ttgtcgaagg cgatatcagt attcattccg
     3301 aatggattga gtatcacgtc aaaaaatgtg atgtcgtctt gcctttggtg gcgatagcca
     3361 cgccgattga atatacccgc aacccgctgc gcgtatttga actcgatttc gaagagaatc
     3421 tgcgcattat ccgctactgc gtgaagtacc gtaagcgaat catcttcccg tcgacttcag
     3481 aagtttatgg gatgtgtagc gataaatact tcgatgagga tcattctaat ttaatcgtcg
     3541 gcccggtgaa taaaccacgc tggatttatt cggtgtctaa acaattactt gatcgagtga
     3601 tctgggccta tggcgaaaaa gaaggtttac agttcaccct cttccgcccg tttaactgga
     3661 tggggccacg actggataac cttaatgcag cgcgaattgg cagctcccgc gctattacgc
     3721 aactcattct caatctggta gaaggttcac cgattaagct gattgatggc ggaaaacaaa
     3781 aacgctgctt tactgatatt cgggatggta tcgaggcgtt ataccgcatt atcgaaaatg
     3841 cgggaaatcg ctgcgatggc gaaattatca acattggcaa tcctgagaac gaagcgagca
     3901 ttgaagaact gggggagatg ctgctggcga gcttcgaaaa acatccgctg cgccattact
     3961 tcccaccgtt tgcgggcttt cgtgttgtcg aaagtagcag ctactatggc aaaggatatc
     4021 aggacgtaga gcatcgtaaa ccgagcatcc gcaatgcccg ccgctgcctg aactgggaac
     4081 cgaaaattga tatgcaggaa accatcgacg aaacgctgga tttcttcctg cgcaccgttg
     4141 atcttacgga taaaccatca tgaccaaagt cggcttacgc attgatgtcg atacctttcg
     4201 tggcacccgt gaaggcgtgc cgcgtctgct ggaaatcttg agtaagcata atattcaggc
     4261 cagcattttt ttcagcgtcg gcccggacaa tatgggccgc catctctggc gactggtgaa
     4321 gccacagttt ttgtggaaga tgctgcgctc aaacgcggca tcgctttatg gctgggatat
     4381 tttactggca ggtacggcct ggccaggtaa agagattggt catgccaatg ctgatatcat
     4441 tcgtgaagcg gctaaacatc acgaagtcgg cctgcacgcc tgggatcacc atgcctggca
     4501 agcccatagc ggtaattggg atcggcaaac aatgatcgac gatattgcac gcggccttcg
     4561 caccctggaa gagattatcg gtcaaccggt aacctgttct gccgctgctg gctggcgtgc
     4621 tgaccaacag gtgatcgaag caaaagaagc gttccatttg cgctacaaca gcgattgtcg
     4681 tggggcgatg ccgtttcgtc cattgctcga atcaggaacc cctggcactg cgcaaattcc
     4741 ggtgacctta cccacctggg atgaagtgat tggtcgggat gtgaaagcag aagattttaa
     4801 cggttggtta ctcaaccgca tccagcgaga taaaggcacg ccggtttata ccattcatgc
     4861 cgaagttgaa ggctgcgctt atcagcataa ttttgtggat ctcctgaaac gcgcagctca
     4921 ggaaggcgtg acattttgcc ctttaagcga actattatca gggacgttgc cgctcggaca
     4981 agttgttcgc ggaaatattg ccggacgtga aggctggctg ggttgccaac aaatggcggg
     5041 tagtcactga tgaaatcggt acgttacctt atcggcctct tcgcatttat tgcctgctat
     5101 tacctgttac cgatcagcac gcgtctgctc tggcaaccag atgaaacgcg ttatgcggaa
     5161 atcagtcggg aaatgctggc atccggcgac tggattgttc cccatctgtt agggctacgt
     5221 tatttcgaaa aacccattgc cggatactgg attaacagca ttgggcaatg gctatttggc
     5281 gcgaataact ttggtgtgcg ggcaggcgtt atctttgcga ccctgttaac tgccgcgctg
     5341 gtgacctggt ttactctgcg cttatggcgc gataaacgtc tggctttact cgccacagta
     5401 atttatctct cattgtttat tgtctatgcc atcggcactt atgccgtgct cgatccgttt
     5461 attgccttct ggctggtggc gggaatgtgc agcttctggc tggcaatgca ggcacagacg
     5521 tggaaaggca aaagcgcagg atttttactg ctgggaatca cctgcggcat gggggtgatg
     5581 accaaaggtt ttctcgccct tgccgtgccg gtattaagcg tgctgccatg ggtagcaacg
     5641 caaaaacgct ggaaagatct ctttatttac ggctggctgg cggttatcag ttgcgtactg
     5701 acggttctcc cctggggact ggcgatagcg cagcgggagc ctgacttctg gcattatttt
     5761 ttctgggttg agcatattca acgctttgca ctggatgatg cccaacatag agcgccgttc
     5821 tggtactact tgccggtcgt cattgccggt agcctgccgt ggctgggatt actccccggt
     5881 gcactgtacg ctggctggaa aaaccgcaag cattccgcaa ccgtctattt attgagctgg
     5941 acgataatgc cgctgctgtt tttctccgtc gctaaaggca aattgcccac ctatattctt
     6001 tcctgctttg cacctctggc aatactgatg gcgcattacg cgttgctggc agcaaaaaat
     6061 aatcctctgg cgttgcggat taatggctgg attaacatcg cttttggcgt cactggcatt
     6121 attgccacat ttgtggtctc cccctgggga ccaatgaaca caccggtgtg gcaaaccttc
     6181 gagagctata aagtcttttg tgcctggtct attttttcgc tatgggcatt tttcggctgg
     6241 tacaccttaa caaacgtcga aaagacctgg ccttttgccg cgctttgccc gctggggctg
     6301 gcgttgctgg taggattttc aattcctgac agagtcatgg aaggaaaaca tccgcaattt
     6361 tttgtcgaga tgacacaaga atcacttcag ccaagccgct atattcttac tgatagcgtc
     6421 ggtgttgccg caggtctggc atggagcctg caacgcgatg acatcatcat gtatcgccag
     6481 acaggtgagt tgaaatatgg ccttaattat ccggatgcaa aagggagatt tgtcagcggt
     6541 gatgagttcg caaactggct taatcaacat cgtcaggagg ggattattac actcgtgctt
     6601 tcggttgacc gcgatgaaga tatcaatagt ctcgccattc cgcccgcaga tgtcatcgat
     6661 cgtcaggagc gtctggtgct aattcagtat cggcccaaat gatctggcta acattagtct
     6721 ttgccagctt gcttagcgtt gccgggcagt tgtgtcagaa acaggcaacc tgctttgcga
     6781 cggtaaacaa acggcgcaaa catatcgtgc tgtggctggg actggcgctg gcttgtcttg
     6841 gccttgccat ggtgctctgg ctgctggtct tgcagaacgt accggtaggc attgcctacc
     6901 cgatgttaag tctgaatttt gtctgggtaa cgctggctgc agtaaaactg tggcacgagc
     6961 cggtatctct gcgtcactgg tgtggggtgg cgttcattat tggcggcatt gtgatcctcg
     7021 ggagtacggt gtaatgggcc tgatgtgggg attattcagc gtcattatcg cctccgctgc
     7081 gcaattaagc ctgggttttg cggcgagtca tctgccgcca atgacgcact tatgggattt
     7141 tatcgcggcg ctgttagctt ttggcctgga tgcccggata ctgttactcg ggctgctggg
     7201 ttatctgctt tccgtttttt gttggtataa aacactacat aaacttgcgc taagcaaagc
     7261 ctatgccttg ctcagtatga gttatgtgct ggtatggatt gcttcaatgg tacttcccgg
     7321 ctgggaaggg actttttcgc tgaaagcact actgggagta gcctgtatta tgagcgggtt
     7381 gatgctgatt ttcctgccca caacaaaaca acgttactga gttttccctg ctgctttaca
     7441 ctgccgttcc cactcatcgg ggctataaca gtttgcagaa aggactttca ccgtgtgcag
     7501 cttttcacga ttaatacagt aaagcgcatt ctgcaaaggc gagagtaaat cccccacttt
     7561 cacggcgaag tcactcttca cttcggcgat catttttatc gcgccgccag catcgcagag
     7621 cataattaca tgtctgttat cttgtttatt gcagcacgat tttttgacca gccattccat
     7681 tccattatcc tgtttgctaa gagttttcac atcaatttac agattaacag ataaactctt
     7741 tatcaacggc aatggaatgg cagaaatgag aagtgaaacg gagggttaat tattgctggc
     7801 gttgcaccca ctcttttagc gcctgacgtg aaattttaat accaccgttt ttcagctccg
     7861 gcggcagagt cagccagcgc accggttgtt gaaaacgagc cagcttatct ttcacccatt
     7921 cgtcaagatc aacgctttgc tggtcatact ccaccaccgc taccggccga tgaccaaact
     7981 ccttgtcggc aacgggaacg ataaacacct gcaaaaccgc aggatgtgca gcaataacgc
     8041 gctcgacttc ttccggctga ataccctcac cgccactgaa gaatagattg tctaaacgtc
     8101 cgacaatggt cagcttgcca ttatgcatct caccgcgatc gcgcgtggcg taccagcctt
     8161 cgtcattaac cagtggaacc cgttgcccgt tacgccagta accttctgcc atactggcag
     8221 cccgcagcca cacttcatca ttaacgattt tcacttcccg acccggcagc ggcgaaccaa
     8281 cgtctgccag accgtcggct tctttcgcac acactgtgga ggcaaactcg gtcagaccat
     8341 agccgcaaaa gcaccgaatc ccctgctcgc gcgcctgttc cgtcaactcg accgggatag
     8401 ccgcgccgcc aagtaaaacc gctttcaggg aaacggaact acggttaacc agcaaacgcc
     8461 agagttgtgt tggcaccagt gaagcgtgag tacagcctgc cagcatctgc tccaatggct
     8521 gtttatcccg caccgtcatc cgcgcaccag cgtataacca gcgccacata attccctgac
     8581 cggagacgtg aaacagcggt aaagagagca accagtcatc gtgatcgcca aacggaatca
     8641 gcgataacac cccttcggcg ctggcaagat gagcctgata agtatgaaca gcggctttcg
     8701 gcaagccagt ggaaccagag gttaacgtca ttgagcacag acgcgtcggc tgccacgccg
     8761 cggcatgtgc gccttcaacc agctgaatgt gcagcgacgc taacgccgga aacgtgtttt
     8821 ccccttccgg caccagcgca aattgcagcg tcagattggg cagcaattcc tcaagcagcg
     8881 gttgcggcag ctgagggttc acgggcaaca cccgcgcccc gcattgcagt aacgccagcc
     8941 aggccagcag cgtttgcggc gtattccacg cccgcaacat cacgccgctg ccctcaacca
     9001 caccctgcac cgcaaagctg gaggctaatt catcgacgcg agcacaaagc tcgcgccagt
     9061 tgagttgctc gtcattgaga cgtaaggcga tggcttctcc ccgcacttgc cgccagtgac
     9121 gccacggcca gtcagagaag atcataacag ccgctccagt gcatcaacgt ccactaacgg
     9181 cagtgggctc cccggccagc gacgaacctg ctgcgcctgc atcagatcca gcgtgtccag
     9241 tcccggaatg gtgtctggcg ttaaccaggc agcaatccgc gccagttgcg ttagacctaa
     9301 gctcgactca atggaagaac tgatcaccgc cgtcagcccc agcgcatgcg ccgcctgcac
     9361 ttgctcgcgt actttatcca ggctgcccgt gagcgtgggt ttgataacta ccgcgcgcac
     9421 gccctcttca gctacaaagg caaaatccgg ctcgcgcaag ctttcatccc aggcaatggc
     9481 aatgccggtt tcacgggcaa acgcacgcga atcatcgcgg gttttgcacg gctcttcgag
     9541 aaacgcgatg cggtggcgat aatccgggtt aacgtatttg gcaaactgct ggcctttcag
     9601 cggtgtccag gcgcgattag cgtcaagacg caaatgcaga tcaggaattg cctccagcaa
     9661 cagattcacc accatgccgt cgcgcacggc ttcgtacaat cccactttga ccttcgccac
     9721 tttctcgcca ggcatatcgg caagtttgag gatcagatcg tcaggatcgc cattacacag
     9781 cggtgccgca cggtaattgg ccgcctgcgg gagcgtatct gccagctccg ccaatgcaca
     9841 acttacgcca aaggcaacgg aaggcatctg cggtatctcg caatcgcctg ccagccagtt
     9901 atttacccag gcaagcagca cactttgcgc ctcttcccag ctttcctgac tgaaccccgg
     9961 cagtggggag atctctcccc acccttcgcg ctcaccctca cgcaggcaaa cgtacagccc
    10021 gtcgcgggtt tttaaccgcc tgtcgcgcag aaccaccccc gcgtccatgg ggatctgcca
    10081 gcggtatacc tgcgcgctac gcattacgga ttccgtttaa atttgctgaa atcaggctga
    10141 cgtttctggt tgaaagcgtt gcgaccttcc tgaccttctt ccgtcatata gaacagcatg
    10201 gtggcgttac ctgccagctc ctgcagacct gcctgaccgt cacagtcggc gttcagcgca
    10261 gctttcaggc agcgcagcgc catcgggctg ttttgcaaca tttcgcggca ccaacggacg
    10321 gtttcttttt ccagatccgc cagcggtaca acggtgttca ccagccccat atccagcgcc
    10381 tgttttgcgt cgtactgacg gcacaggaac cagatttcac gtgctttttt ctgcccaaca
    10441 atgcgcgcca tgtaggaagc gccccagccg ccgtcgaagg aaccgacttt cgggccagtc
    10501 tgaccgaaga tggcattatc agccgcgata gtcaggtcgc acatcatgtg cagaacgtga
    10561 ccgccgccga tggagtagcc agccaccatc gcgacaaccg gtttcggaca ggtacggatc
    10621 tgacgctgga agtccagcac gttcaggtga tgtacgccgg aatcatcttt atagccgccg
    10681 taatcgccac gcactttctg gtcaccaccg gagcagaacg ctttatcgcc tgcaccagtc
    10741 agaatgatca cgccgatgtt gtcgtcataa cgcgcatccg ccagcgcctg gatcatctct
    10801 tttaccgtta gaggacggaa ggcattgcgc acctgcggac gattaatggt gatttttgcg
    10861 ataccgtcgg tggatttttc ataacgaatg tcctcaaaac cttcggagca gtcgtgccat
    10921 tcaaccggtg cgtaaagcat tgcttcatca ggataaatca tattgtgtcc tttagtcaga
    10981 aacgcaagat ctgcgccaga cttgcgatta cgccagcggg attttcccgg tgcgcgttat
    11041 gtccggcgcg aggaatgaca tggcagtcgg cagccagttc cgccgccaga gcgcggaatt
    11101 tgctgtcacg ttcaccacat aaataataaa acgcaaatgt gcgggcgcta aggttagcac
    11161 gtaaatcggg ctggacggcg agagaagtcg cctccagcat ggcggcaagg gttgcgccat
    11221 tattgttgct gcgcagcgcc accagctccc ggcgttgatc gtcattgagc gaggcaaaaa
    11281 caggctgttg ataccaatcg gcaaataccg ccgttaacgg ttctgtacga aaacgctgcg
    11341 cccattggcg atcggaacgc tgacgttccg cgcgttgttc agcattttgc aacccaggat
    11401 gcccgccttc gacaacaact ccacaaagcc ccgccagctc ctggcaagcc gccatcatcg
    11461 ccacccgtcc gccaagcgag taccccacca gccagaagtt aaggatgttg taactaacca
    11521 gcgttttaca cagtaagtcg gtgacatcat caaatccatc gacgctgatc gtcgctgaac
    11581 caccgtgacc tgggagatca acatacaacc gcgagtagtc ggcaaacgcc tcacccactt
    11641 cttgccattc atggcaatcg ccggaaaaac cgtgcaaaaa caccagccag ggtaaacctg
    11701 gtttgccgtg ttttgcctgc gcgtgcagga tcataaatgg cttacctgcg ccagaagttg
    11761 ctggagcgtt tgcgcaccat cggtgtcgtt aaccaccatt tcaattaccg tggtggtcgg
    11821 cgtgcgccag gcgtcggcaa gtgccgtttc aagttcctgc cagttttgcg gacgatgata
    11881 tttcagctcg aacatcgcgg cggcgtgctc aaaatggacg ttttgcggca tcagatagaa
    11941 acgctcgcgc tcgtttttcg gcgttggcaa cagcgagaaa atttgcccgc cgttgttgtt
    12001 caccacaatt aataccagcg gcgcggaaac ctgacgtaat aacgccatcg cgttgagatc
    12061 gtaaagagct gagagatcgc ccactatcgc cagcgtcggt ttgccgcttg cccgctgtac
    12121 gcctgcggcg gtcgaaagca gtccgtcgat accgctggcc ccacggttgc tgtacaccgg
    12181 gtaaccagcc ggaagttgcg aaagcgcatc tatcaaacgt accaccaggc tgttgccaac
    12241 aaacagttgc ccctgttcag gtaaatagtc gcagatgcga tgcgccagtt gcgcttcgcc
    12301 aaacgcatcg cgacgggcaa taaccgcctg cattgcctgt tccgccaggc gcgggatttc
    12361 aacgcaccag ggctggcgtt tttctgccgg atgcagctcc agccagtcgg caatattggc
    12421 aattaagcga cgtccgcgat ggtgtgccgg atcaagtcgc ccttcaatgt catcgacaat
    12481 ccagtactct tctggttcac agcttgcctg ccattggagg agtcgtttcc ccgtcaggct
    12541 gcttcccagt tgcaccacta tttgtgcctg ttgcagttcg ctggtcgctt tggcattgcc
    12601 taaccagaga tcggcacacg gcagcggctg accggtttgc gacagcacgt cgccaatcag
    12661 cggccagcca agagtttgcg cccacagtgc aacttttttg ccctcttccg cactcatgcg
    12721 cccggcaacc accacgccgc gcttttgtcg ccagaagaac cagtcgcgct gtttttcact
    12781 ttcccgacga ggcgcttcac gcagccacgg tttgtcgtcc tgccaccagt cgcccagccg
    12841 ctgttgccag ctaatcccgg tgtcgtccat ttcgccatac agcggttcag caaacgggca
    12901 gttgatatgg actccaccag catgaagcgt accgagagcg tggtcgatgg tagaaaccag
    12961 ccaacgtgcg gggatatcct gggtcgggcg cggcagtgaa atactatgcg tggggtgaga
    13021 ggcgaacatt cccggctgac gaatcgcctg attcgcgccg cagtcaatca gctccggcgg
    13081 gcgatcggcg gttaacagga tcagtttttc tccggttaac ccagcttcaa tcagtgccgg
    13141 atagagattt gctaccgccg tgccggaggt cacaatcacc gccaccggct gcttgctgac
    13201 tttcgccagc cccagtgcca gatgccccag tccacgctca tcgaaatggg tatgatgaat
    13261 gaatgcggaa ttctccgccg ccgctaacgt taacggtgta gaacgcgagc caggggcgat
    13321 acagatgtgt ctgacgccgt gacgcgttaa tgcttccaga atgaccgccg cccagcgtcg
    13381 gttaaatgcg cttactgaca tgagtttgtc cggtatcaat attgcggcta agtataagga
    13441 gctacaaaaa tagattattg atatgaatcg gtaatgatgc gactcattac tattccattt
    13501 gtaataaagt acgcagccct gccgctttgt tgtcgatttc ctgccactct tgctcggggt
    13561 cggaaccacg gacaatgcct gcgccagcat atagccgcac gatattgccg ctgatttttg
    13621 ctgagcgcag ggaaacgcag aattcacttt gctgtaatga gagatagccc gcagaaccgg
    13681 cgtaccattc tcgagtgaac ggttcgtgac gggcgataaa ctgtcgcgcc agatcgcgcg
    13741 gtaagccagc aactgctgcc gttggctgca actgatgtaa acagatcaca tcatccgctt
    13801 tgttgagtga agtccagata cagcggcgaa gatgctgtac tttacgtaga cgcagtacct
    13861 gcggcggtaa aacatccagc gtttgggtat cggcctgcaa tcgttggcag atatcttcca
    13921 ccaccaacat gttctcgcgc tggtttttat catccgccat cagccactca cctaactgct
    13981 gcgcctgctt atcatcagga tgatttgcta ccgttcccgc cagcgcttcg gtacgcagcg
    14041 ctttgtcacg ccgccgccat aaccgttccg gtgaagaacc aagaaaagca ttttcgccat
    14101 caaaggccat gtaaaaatgg tagcaattca gattgagccg acgactggca gccatcatcg
    14161 ctgccgcatt gaccggactt gcgaaatgca ggtcggttgc ccgagcaagc acaactttgt
    14221 cgagctcgcc ttcggcgatg gttttcgttg ccagttcgat taattgcgtc cagcccgttt
    14281 tgtccggcca gtgttgttct cgcgtggtgg ttaaatgtaa tccaggcagg ggcttgatac
    14341 tcaccagtgt ggcgataaat tcttttgcct tgattgcatc gtgctgaagg gagctttcgc
    14401 tgaatagcgt cagccgcagc gtggctttac cgccacaacg tcgccattcc aggcgtggta
    14461 aaagtaaatt gccctgcgac gggtcaaacg cattcaaccc ccagatgcgt aagtcggcgt
    14521 gttccgggtg ctggcgaaga aaacgttgtg cctggtccaa cgacgtaaaa cgggtaatcg
    14581 cacccaggac ggcagcttct tcatcaccat tacgttgttg ccagtagaat tgcggataca
    14641 cctgctgact ggccagccag ctcaaggcat caaaagcgtc tttgagaggg aaaggaatat
    14701 cgataacccg aatgccgggt gtcgccggaa tctcttgcga caaatggcgc agtagatttt
    14761 ccagcgccgt agtaagtgat tgcacgcgga cctctccctg ttaaaagcct cacattatac
    14821 ggggtacgac aaaaaaaatg cagtaccccg gtgtagggag gttttaacgg cgtgccagca
    14881 acagtcccag aaccagccca acggccgcac ccacaccaat tccttgccag ggtttttcgt
    14941 ggacgtagtc atcagcacga taaacagcct gcttcgcccg atagtaataa ctgtctgaag
    15001 cctggctaac ccgttttttc acatcatcca gcgctttttc tgcacgcgct ttcagctcaa
    15061 catatttctg atcggcggga tcgccagagg agcggagcac ctcttccagt gtttcactaa
    15121 gcagcgtcag gtcatcatca atacgtgtat caccaaactg attagacatt ctcgttctcc
    15181 atttgtgtaa aacctgtcac taactataga caagggttat tgattacgcc tgaattacct
    15241 cgcgtgccat cccaatgtgt gggataccat cttcttcata aatatcggtc acagggatga
    15301 aaccaaattg cccataaaag ttttgtaaat gcgcctgcgc ccccagataa atcggcttct
    15361 ccggccagcg gcgagtacac gattccagcg ccttactcat caattgctga ccgatttttt
    15421 caccgcgtaa ggcttcgctg acaatcaccc gaccaatcac aacgggttgc agttcgtcgt
    15481 cacttttcag aatcctcgca tacgccacca gtgtcccgtt atgccaaccg agaatgtggc
    15541 gattttctcc ctccagatcg tcaccatcga tatcctggta aggacagttt tgttccacta
    15601 caaataccgc acaacgcagt tgtaataagg cataaagctg agaaacggat aaatcagaat
    15661 gatgcagatc ttgccagtcg atcatggaag gctcctttgt gctgtttgct ttatactagc
    15721 cggaatattt tcagcagcgt gatgaactca tggaattaat ttttttaggt acttcagccg
    15781 gtgtgccaac ccgtacgcgt aatgtcacgg caatattgct taacctgcaa catccgaccc
    15841 agagcggact ttggttattt gactgcggtg aaggcaccca gcatcagcta ctgcataccg
    15901 cctttaaccc tggaaaactg gacaagattt ttatcagtca ccttcatggc gatcatctct
    15961 ttggtttacc cggcttgctg tgcagtcgtt ctatgtcagg cattatccaa cccttaacga
    16021 tttatggtcc gcacggtatc cgtgaatttg tggagaccgc gctgcggatt agcggctcat
    16081 ggaccgatta tccgctggaa attgtcgaaa ttggcgctgg cgaaattttc gatgatggcc
    16141 tgcgcaaagt gaccgcttat ccgatggaac acccactgga atgttatggt tatcgtattg
    16201 aagaacatga taaaccaggt gcattaaatg cccaggcatt aaaagctgct ggcgtgccgc
    16261 ctggcccgct gtttcaggaa ttaaaagcgg gcaaaacaat catgctggat gatggaaggc
    16321 agattaatgg cgcagattac ttagccgctc cagtgccggg taaagcgctc gctattttcg
    16381 gcgatacggg cccctgcgat gccgcgctgg agctggctaa aggcgtcgac gtaatggttc
    16441 acgaagcgac tctggatatg gcgatggagg ccaaagccaa cagtcgcggc catagttcta
    16501 cacgccaggc ggcggcacta gcccgtgagg ctggagtcgg taagctaatc attacccacg
    16561 tcagctcgcg ctatgatgac aaaggttgtc agcacctgtt acgtgaatgt cggtcaattt
    16621 tcccggcgac tgaactggcg aatgatttcg cagtgtttaa cgtttaactc tgcttattat
    16681 taaaaacagg gcgaaacttg ccctgttatc gcaacccgcg ctacatatat ccacccacac
    16741 gggtatattg agcgattttt tatatttatt catctcctca cgcactatat tcagagctat
    16801 aagttgcctc tttttatctt gattcagcac cacctttgca actaaaagga gcctatgatg
    16861 gttacagttg tcagcaatta ttgtcaatta tctcaaaccc aactctgtca gacatttgca
    16921 gaaaaattta ctgtgaccga ggaattactg ctgtctttaa aaaaacactc ataactcatt
    16981 acaaataatg atgaaataac gccttttatt gataatctac atatgaaaaa aatgacatct
    17041 ctttgtttta taccattact actatcgctt acttttatta ttttttacca accctgggtc
    17101 agggcattac caccaacgcc ccgacacgca agtcctgagc aattagaaaa aacagttcgt
    17161 tatcttacac aaactgtgca tccacgtagt gccgacaata ttgataatct gaataggtcc
    17221 gcagagtata taaaagaggc ctttatcagt cgcggagcca gaattaccgc gcaggatatc
    17281 cccattacgg gtggccccta taaaaacatt gttgctaatt atggtcctgc cgatggaccg
    17341 ctgattatta ttggtgcgca ctatgactct gtcagtagtt atgaaaacga tcaattgacc
    17401 tatacgccgg gcgcggatga taacgccagc ggtgtggcag gattactcga actggcacgt
    17461 ttgttacagc agcagactcc agggattggc gtccaactgg tcgcctatgc gtcggaagaa
    17521 ccgcccttct ttcgtagcga tgaaatgggg agcgcggtac atgcagcttc gcttgagcgt
    17581 ccagtgaaat taatgatagc gctggagatg attggttatt acgactctac gcctggtagc
    17641 caggattacc catccccggc aatgtcctgg ctttatcccg atcggggaga ctttattgcc
    17701 gtggtcggca gaatgcagga tatcaacgcc gttcgtcagg taaaagcggc attgttgtca
    17761 tcccgggatt tatctgttta ttctatgaat gccccaggtt ttattcccgg tattgatttc
    17821 tctgatcacc ttaattattg gcaacacgat attcccgccg taatgattac tgacaccgct
    17881 ttttatcgta ataaacaata ccacttgccc ggtgatatcg cagacagatt aaattatcag
    17941 aaaatggctc aggtagtgga tggagttaca accttgttat acaacagtaa ataataaaaa
    18001 tttctcgagg cggatccaga aaaagcaaca tgcgcatttt attttctttc gtctgtttta
    18061 agatgcgaaa catgaaattc aacggaggaa aaataaatgg gaatgattgg ctattttgca
    18121 gaaattgact cagaaaagat taaccaactg cttgagtcta ctaaaaaacc tttgatggat
    18181 aatatacatg acacgctttc aggattacgc cgactggata ttgataaacg ctgggatttt
    18241 ttacactttg gcctgacggg tacatctgca tttgaccccg cgaagaatga cccgctaagt
    18301 cgcgcagtgc ttggtgaaca cagcctagaa gatggcatag atggcttcct gggattgacc
    18361 tggaatcagg agttagccgc gacaatcgac cgactggaat cactggatcg aagtgagctg
    18421 cgtaaacagt tttccattaa gcgactcaat gaaatggaga tttatccggg agtaaccttt
    18481 tcagaagagt tagaggggca actctttgct tcgatcatgc ttgatatgga aaagctgatt
    18541 agcgcgtatc gacgtatgct gcgacaaggg aatcatgcgc tgacggttat tgttgggtga
    18601 tagtgtcagg gcaccaaaaa aaaccgccga acatgtcggc ggctttctga cttacaaact
    18661 gactgcttac atcagcggca ttgctaaccg cacaatgcta atcagcggtt gtggccatac
    18721 acccagcacc agtaccaaca gtgcagagat cagcaccacg ataccgcctg cgctgtactg
    18781 ccagtttgat ggtgcatcgc gacccggttg ttccggagcg tgaaggtata agctcaccgc
    18841 cacgcgcagg tagtagtaga ggccgattgc cgaaccgaca actacggcac ccaccagcca
    18901 ccacaagtgt gcctggacac cgaccgccag cacgtagaac ttaccgataa agcccagcgt
    18961 cattgggata ccagccagtg aaagcatcat caccgtcatc actgccgcga ggatcggacg
    19021 atgccagaac agaccgcggt aagagaacag ggaatcagca tccgggccac gatacgggct
    19081 ggacatcagg ctgaccacgc cgaacgcgcc gaggctgctg aacagataac cgaccaggta
    19141 aacccctacc gcttccatcg acatctcgcc ggtttgcagc gcaatcagcg ccaccagcag
    19201 atagccgagg tgagagatag atgagtaacc gagcagacgt ttgatattgg tctggctcag
    19261 cgccatcagg ttaccgaaga tgatagaggc aaaggcgata atcgccagca ctacgcgaat
    19321 cgcttcactg tcacccaccg gtgcgtaaag gaacaaacgc atgaccacgc cgaagatagc
    19381 gattttgctc gccgtcgcca ggaaagtgga aaccggcgca ggcgcgccct ggtatacgtc
    19441 tggcgtccac aggtggaacg gcaccagaga gagtttgaaa ccgaggccaa caatcatcag
    19501 gccaaaacct gccagcaaca gcggctcgtt gagcataccg tcgccgaggt ttttacccag
    19561 tgcgacaaac gacaggtcgc cagactgcgc atacaccagc gccataccga acagcaggaa
    19621 agaagacgct gcggcagaaa ggatggtgta tttgatactg gcttccagcg aacgtttctg
    19681 gcggaaagcg taaccgacca ggccaaacag cggcaaagag atcagttcaa taccgaggaa
    19741 cagagacgcc agatggttgg cattcgccag caggatcccg cccagcgcgg caattaacac
    19801 caacaggtag aactcgtcct tgttgtcgtt atagccttca agccacgggt aggcgaaagt
    19861 acaggtggcg aggctcgcca acaataccag cccggtgtaa agcatggcga aaccatcaac
    19921 gcgcatcagc ggcgtaacgt ccatagcgcc cgcctggccg acaaaccaga gcgataccag
    19981 cgccgcgtta agcccaataa ccgagagcgt agcgttgagg aaatgattgc gtcgccacgc
    20041 aatggagagc atcacaacca ccaccgtcaa gccgacgatc agcaacggta gcagtgcgat
    20101 caggttttgt ggagttattg tcatggcgat ttacggcctt gtagtagtaa cggaattaac
    20161 aaaccactgc tggatattgc caatcgcgga gtgcgaggta tccagaatcg gctgcggata
    20221 gaagcccagc agtaccagca gcaccaccag caacaggatc ataaacagct cacgcagcga
    20281 catccctggc agttcctggc tggcaatctg gcttttcgct ttaccgaagt aagcgcgatg
    20341 cagcatcgcc agcgaataaa cagaagcaaa gaccagccca aaggtagaga taacggtaat
    20401 caccgggaca acctggaagc tgccgaacag aatcataaat tcgccgacga agttaccggt
    20461 gccaggcatc ccaagcgttg ccaccgcaaa gaacagcgac agtgctggca gccatttcat
    20521 tttgctccac agaccgccca tcatgcgcat gtcgcgggta tggatacgtt cataaagctg
    20581 accacaaaga ataaacagac ccgccgccga caagccgtgc gcaatcatct ggattaccgc
    20641 gccctggtag gccaactggc tgccggtgta gatagcaatc agcacgaagc ccatgtggga
    20701 aaccgaggtg taggcgatca gacgtttgat atcggtctgg gcgaaggcca tccacgcacc
    20761 atagaagatg ccgataacac ccagccacat agcgattggc gcgaactctg ccgacgcgtt
    20821 cgggaacagc ggtagggaga aacgcagcag accgtaagcg gcggttttca gcaagatccc
    20881 cgcgaggtca acggaaccgg cggtcggtgc ctgggagtgc gcatccggta accagccatg
    20941 cagcggaacc accggcattt tcactgcgaa ggcgatgaag aagcccagca tcaacaggta
    21001 ttccacgccg ttagacatcg gcgtattcag cagctcttca tagttgaagg tccagacgcc
    21061 ggtcgcattg tagtgaacga aaaccagcac caggatggcg atcaacatca ccagaccact
    21121 cgcctgggtg taaatgaaga acttggttgc cgccgtgata cgcgttttac cgtcagaggc
    21181 tttatgcccc cacagtgcga tcaggaagta catcggcacc agcatcattt cccagaagaa
    21241 gaagaacagg aacatgtcga tggcaaggaa cacgccgata acgccgccca ggatccacat
    21301 caggttgagg tggaagaagc cctgatattt ttcgatctct ttccacgaac agagtaccgc
    21361 cagcacaccg agcagaccgg tcagcacgac catcagcaac gacagaccgt caatggcgag
    21421 atgaatagag ataccaaaac gcgggatcca cggcatgtcg aattcagact gccactgcgg
    21481 aattccggcg gattgcgtca gtgaataacc gccctgcaac cacagttgca gcgacagcgc
    21541 cagcgtcaat cccatggtta ccagcgcgat ccagcgcggc accttgacgc caaagcgttc
    21601 ggtctgccag cacaggaagc cgccgataaa gggaattaat attagccagg gtagtaacat
    21661 ggcgatcttt attccttgta aaagtcccgt caggaccgga ttttcaacga attctcacga
    21721 cagaaaattc tcttcggact gggggtagtg tcggatgcgc cgcgaagacg gcttatccga
    21781 cctacgttct tatatgccca ggggcaatcc cacaatcctt aactcaacgc agtaccatca
    21841 acagtgccag caccacgacc gcaccgatgc tcatggatgc cacataccag cgcagataac
    21901 cgttctcgct taacagcaga cctttacctg caaagcggga aaggacagcc gggatgttca
    21961 tcattgagtt cagcggatcg cgtttcagca accaggcaat acccaggaac ggcttgacga
    22021 acactttgtc gtacagccag tcaaagcccc aggcgttgta ccaccaggta cccagcagac
    22081 ggcccggcgc actgttggcg atggaggtca ccagagtacg tttacccagc cacagccagg
    22141 cagccagcag aatgccgacc accgcgacca cgccagaggt aatttccagg gtcaacatgc
    22201 tgccgtgcgc cagttccgtc gtttgcggaa gcacgccctg cagcggcggt acaatcagtg
    22261 cgccaacgaa ggtggaaagg atcagcagca caatcagcgg caggctgtga gttacccctt
    22321 tcacggcgtg agcgtgaatt tgttcttttc cgtggaagac gatgaaaatc atacggaagg
    22381 tgtaaagcga ggtcataaat gcaccgacca gacctgccac catcagattg atatgtccat
    22441 tcgccatcgc acccgcgagg atctcatcct tactgaagaa gcccgcagtg accagcggca
    22501 gtgccgacag tgctgcgccg cccaccagga agcagagata aaccagcgga atagatttac
    22561 gcagaccacc catcttaaag atgttctgtt cgtgatggca ggccagaatg acggaaccgg
    22621 atgccaggaa cagcagtgct ttaaagaacg cgtgggtcat caagtggaaa atcgccgcat
    22681 cccatgcctg cacgccaagc gcgaggaaca tgtagccaat ctggctcatg gtagagtaag
    22741 cgagaacacg tttgatgtcg gtctgtacca gcgccgcaaa accggccagc agcagcgtaa
    22801 ccgccccaac aatgcccacc agatgcagaa cttccggcgt catcaggaac agaccgtggg
    22861 tacgggcgat caggtagaca cccgcggtta ccattgttgc agcgtggatc agcgcggaga
    22921 caggcgtcgg gcccgccatc gcgtcggcaa gccatgtctg caacggcaac tgagcagatt
    22981 taccgaccgc accgcccagc agcatcagcg tcgcccacat cagcatgtta ttgccgtcag
    23041 caaagtgcgc tggtgccagt tccaccattt cgcggaagtt cagggtgccc agttcgttgt
    23101 aaagaatgaa cagcgcgaaa gcgaggaaga cgtcacccac acgggtcacg acgaacgctt
    23161 tcattgccgc tgcgccattc ttcggatcgg tgtaatagaa accgatcagc agataggagc
    23221 acaggcccac gccttcccag ccgaggtaca tcagcagcag gttatcggcg agaaccagaa
    23281 ctaccatgct agcgatgaac aagttggtgt aagcgaagaa acgagagtag ccctcttctc
    23341 cgcgcatata ccaggaggca tacatgtgaa taaggaaacc cacaccagtg accactgaga
    23401 gcatggtcag cgacaggccg tccagcacca gattgaaacc aatgttaaag tcgcctaccg
    23461 acatccacgt ccacagcggc tggctgtatg cctgctcacc gttagcgaag aagtcaacgc
    23521 cgataaaggc ggttaccagc gccgccaggc ccacagagcc aacgccgacg atcgccgaga
    23581 cgttttcaga ccagcgccca cgggagaacg ccagcaggac gaagccaatc aatggcaaaa
    23641 taatggttaa ggcaagcatg ttcatccgcg catctcactt actgaatcga tgttcaggtt
    23701 ctggcgacga cggtgaagtt gcagcagcag cgcaaggccg atactcgctt ccgccgccgc
    23761 gaggctgatg gcgagaatgt acatcacctg accgtcggtc tggccccagt agcttccggc
    23821 gaccacaaag gccagcgcag aggcgttaat catgatttcc agaccaatca gcataaacag
    23881 cagattgcga cggataacca gaccggttaa gccaagaacg aataagattg ccgcgaggat
    23941 cagtccatgt tgtaagggga tcatgcgtgc tcctccgttt ttcttttcgc gctgtcgtct
    24001 ttacgattgc tcagcacttc acccgctcgc tcttcacgac caacgtggaa ggccacaacc
    24061 agacccgcga gcagcagcat agaagccaat tccaccgcca gtacgtaagg cccgaacagc
    24121 gtaataccca ctgctttagc actgactggc gtaccgtcga taccctgatc gttaacaccg
    24181 agaatggcgt aaacaatcac caccagcatg atggccgaca aaattgccgg accaatccac
    24241 acctgcggtt tcagccactg gcgttcctgt tcgatttccg aaccgcccag gttgagcatc
    24301 atcaccacga acacgaacag caccataatg gcacccgcgt agacgataat ttccagcgca
    24361 ccggcgaagt aagcgcccag tgagaagaac accccagaga tcgccagcag cgaaataatc
    24421 aggtacagca gtgcgtgtac cggattggta tgggtgatca ctcgcaaggt cgcaagtatg
    24481 gctatcaggc cacagatata aaaagcgaac tccattgccc ctctccttac ggtaacaggc
    24541 tcttgacgtc gataggcttg gcttcgttct ctgcttcgcc cttatctttg ccgtcgattg
    24601 ccatacctgc catccggtag aagttatatt ccgggtattt gcccggaccg gagatcagca
    24661 gatcctcttt ctcgtaaacc agatcctggc gcttgtattc tcccatttcg aaatccgggg
    24721 ttaactgaat cgccgtggtc ggacaggctt cttcgcacag accacagaaa atgcagcgtg
    24781 agaagttgat gcggaaaaat tccggatacc agcgaccgtc tttggtttct gctttttgca
    24841 gcgagataca gccgaccggg caggctaccg cgcagaggtt acaggctacg caacgctctt
    24901 cgccgtccgg atcgcgggtc agaacgatac gaccacgata acggggcggc agatagaccg
    24961 gctcttccgg gtacattcgc gtttcgcgtt tggcgaacgc gtgcaggccg atcatccaga
    25021 tactacgaac ctgggtgccg aaacctacta acaattcttt taaggtcatg gtcttattgc
    25081 cccttattgc gcctgccaga gaatgacagc cgccgttacc agcaagttga tcagcgtcag
    25141 cggcaggcag attttccagc cgaaggacat tacctggtca taacgcggac gcggtaacga
    25201 cgcacgaatc aaaatgaaca tcatcataaa gaacgcggtt ttcagcgcga accagatgaa
    25261 tggcggtaac aacgggcctt gccagccacc gaagaacagc gtcaccatca gtgcagagat
    25321 ggtcacaatc ccgatgtatt cgcccacgaa gaacagaccg aacttcatac cggaatattc
    25381 aatgtggtaa ccatccgcca gttcctgctc ggcttccggc tggtcaaacg ggtgacggtg
    25441 acacaccgcc acgcccgcga tggcaaaggt aataaaacca aagaattgcg ggatgacgtt
    25501 ccacacatgc gcctggctgt tgacgatgtc ggtcatgttg aatgaaccgg cctgcgccac
    25561 cacgcccatc agggaaagcc cgaggaacac ttcgtagctc agggtctgcg cagaagcacg
    25621 catcgcaccc agcagcgagt atttgttgtt acttgaccag cccgcaaaca gcaccgcgta
    25681 aaccgccaga cctgccatca tcaggaagaa caaaatcccg atgttcaggt cggcaaccac
    25741 ccaacccgga ctgactggca caattgcaaa ggccagcagc agcgaggtaa aggcaatcat
    25801 cggtgccagg gtaaagatga cgcgatccga gaatttcggg atccagtctt ctttaaagaa
    25861 cattttgatc atgtccgcaa ccagctggag cgaaccgccc cagccaacac ggttaggtcc
    25921 ataacggttc tggaacagac ccagcaggcg acgttcgcca aagctcatga atgccccaca
    25981 ggtgactacc accagcagga tcaccaccgc tttaaggacg gtcagcagga tctcaatcag
    26041 ttccggtgat atccaactca ttgttgtgcc tccttgagat cctcaagatg cgcgccagcc
    26101 agcaccggag caatgccgga catacccatc ggcaagccca cctgccctgc cgtcagccct
    26161 tcggagattt caaccggcag cgtgaccatg ttgccatcgt aactaaagga gacgcgtgta
    26221 cctgcgttca cccccaactt cgcggcatcc gctgggttga gtttgatgta cggctgcggc
    26281 atacggctct ggaagaccgg agcacgctgt gacaattcat cgctgccaaa caggtggtag
    26341 tacggcgcga tacgccattt cccgtcctgc ggctggaagc gtgccggtac gctggtgaag
    26401 taatccagac cgttttcgct ggtttcaaac agacgcacgc ccggatcgcc aaaacgcagt
    26461 ttgccgccca cttcgtcctg gaatttgttc cacgcctgcg gggagttcca gcctggcgcc
    26521 caggcaaacg gcacttgcga acggtgcgcg gtcggctggt tgttaccttc catcgagaag
    26581 gtaaacatgg tgtcaatatc ctgcggctga cgcggctcat gaacgctgat attggcgcgc
    26641 atggcggtac gaccgctata gcgatgcggt tcacgggcca gtttctgccc acgaatacgg
    26701 aatgtcgcat ccggcgcagc atctttgata cctgccagtt ccgggatttt cgccacaaca
    26761 gcgtcaatca cgtggtcgag ctgcgtccag tccacttcac ggctcagcag ggtgctatgc
    26821 agcgagtgca gccagcgcca gctttccagc atgacagttt tgctgtcgta ataagccgga
    26881 tcgtaaacct ggaagaaacg ttgggcgcgg ccttcgttgt tgatcaccgt accgtcgctt
    26941 tcagcaaagc tggcggcgga aagtaccaga tgggcgtttt ccataatcgc tgtgcgttga
    27001 tggtcaacca ccatcaccag cggcgcttta gccagcgcag cattcacgcg ggtagcggaa
    27061 gcgtgacgat gcagatcgtt ttccagcacc accaccgcat cggcgcgtcc ggtttccagt
    27121 tcggttaacg cttcttcaag cgatccaccg cccataatgc ccagccccat gctgttgacg
    27181 gaacgggcaa tcatggtgat accgacgtca gcgccgcgac ctttcagggc ttttgcgacg
    27241 ttagccgcag cctgaatcac ttcagcacta ccagcgttcg tcccggagat aatcaacggt
    27301 ttcttcgcac cagccagtgc ctgcacgatg acgtcgattt tgctttgcag ctcaggctct
    27361 atgccgtcaa ctgccggtgc ggagttgtcc agcgcatggg cgatggcaaa acctaaacgc
    27421 gcctgatctt caaccggtgc gcggtaagtc cacgccgcga tatcatccag acgggtgtca
    27481 tcaacgttgg taacaaacag tggatgcttc gcacgttgac cgatgttgag gattgccgca
    27541 atctgccagt cagccacttt ctgtgctgcc gccatttcgc gtgctttacc ttttaccgcc
    27601 tggcgcaccg ccagcgcaac gcgcgcgccg gtctgggtaa cgtcttcgcc cagcaccagt
    27661 accgcatcgt aagattcgat ttcgcgcaga gccggagtat aaatgccgcc ttcacgcagt
    27721 actttcagcg ccagttgcag acgttcctgc tcaccgtgag cgataccggt atagaagttt
    27781 tcttcgccca ccagctcacg cagcgcaaag ttgctttcca cgctggcacg cggagaacca
    27841 ataccgatca ctttcttcga ctgacgcaga atatctgctg caccctgcat tgcctgttcg
    27901 gcgttgaggg taatgaaatc atcgccacga cgctgtactg gctgacgcgg acgatctttc
    27961 aggttgacgt aaccgtaacc gaaacgacca cggtcgcaga ggaagtagtg gtttaccgta
    28021 ccgttgtaac ggttttcgat acgacgcagt tcgccataac gctcgccggg gctgatgtta
    28081 cagccgatgg aacattgctg gcagatgctc ggcgcaaact gcatatccca tttacggttg
    28141 taacgctcgg agtgcgtttt gtcggtgaat acgccggtcg ggcaaatttc gaccaggtta
    28201 ccggaaaatt cgctttccag cgtgccgtct tccgggcgac cgaagtagac gttgtcgtgc
    28261 gcaccgtaaa cgcccaggtc cgtaccgtca gcgtaatctt tgtagtaacg cacacagcgg
    28321 tagcaggcga tgcagcggtt catttcgtga gagatgaacg gccctaaatc ctgattacgg
    28381 tgggtacgtt tggtgaaacg gtagcgacgg aagctgtgtc cggtcatcac agtcatatcc
    28441 tgaagatggc agttaccgcc ctcttcacat accggacagt cgtgcgggtg gttggtcatc
    28501 aaccactcga ccacgctttc acggaactgt ttcgcttctt cgtcgtcaat ggaaataaag
    28561 gtgccatcgg aagccggtgt catacaggac atcaccaggc gaccacgcgt gtcttccgcg
    28621 ttttggtatt gcttcaccgc acactggcgg caagcaccga cgcttcccag cgccggatgc
    28681 cagcaaaagt aaggaatatc aaggcccaga gacagacaag cttccagcag gttgtccgct
    28741 ccgttgacct cgtattcttt gccgtctaca tgaattgtag ccattagcat gcttccagtt
    28801 ttctcagtca gagactgagc gttaatcgaa attcggttac cagcgctctt tcagcaggtt
    28861 cggctgaatc ccattaatca aatgggtatt gctgaacggc tgtttgattc ccgcctcaaa
    28921 ttcttcgcgg aaatatttga tggcgctctg taacggctcc actgcaccag gtgcgtgggc
    28981 acagaaagtt ttacccgggc ctaagaatcg acacagttgc tcaagtgttt cgatatcgcc
    29041 cggctggcct tcgccacgct ccagcgcacg cagaattttc acgctccacg gcagaccgtc
    29101 gcggcatggc gtacaccagc cgcaggactc acgggcgaaa aactcttcca ggttacgcac
    29161 cagcgacacc atgttgatct cgtggtcaac cgccatcgcc agcgccgtac ccagacggct
    29221 tcccgcttta ccgatacttt cgaattccat cggcaggtca aggtgcgctt cggtcaggaa
    29281 gtcagtcccc gcgccgcctg gctgccaggc tttgaatttc agaccgtcac gcataccacc
    29341 ggcgtagtct tcgaggatct cgcgtgcggt ggtgccgaac ggcagttccc acagacccgg
    29401 atttttcacc cgaccagaga agcccatcag cttggtgcca gcatctttac ttttcgagat
    29461 gttctgatac cactccacgc cgttagcgag gatcgccgga acgttacaca gggtttcgac
    29521 gttgttaacg caggttggtt taccccatgc accggaggtt gccgggaatg gcggcttcga
    29581 gcgcgggtta gcgcggcgcc cttccaggga gttaattaac gccgtttctt caccgcagat
    29641 gtagcgccct gccccggtat ggacgaacag ttcgaaatca aaacctgttc ccataatgtt
    29701 tttgccaagc agacccgctt cggtggcttc ggcaatggca cgacgcagat tcactgccgc
    29761 ttcgatatat tcgccgcgca ggaagatata gccacggtaa gctttcagcg caaacgcgga
    29821 gatgagcatg ccttccacca gcaggtgcgg cagttgctcc atcagcagac ggtctttata
    29881 ggtgcccggc tccatttcat cagcgttaca cagtaggtaa cggatattca tggattcgtc
    29941 tttcggcatc aggctccact tcaggccagt cgagaagcct gcgccaccgc gccctttcag
    30001 accagagtct tttacctgat taacgatttc gtccggagac aacccggtca gcgccttacg
    30061 cgcgccttcg taaccgtttt tgctgcgata ttcgtccagc cacactggct gtttgtcatc
    30121 gcgcagacgc caggtcagcg gatgcgtttc gggagtacgg ataatgtttt tcatttatac
    30181 cgctccagca gttcagggat cgcttccggg gtcagatgcg cgtgagtgtc ctcatcgatc
    30241 atcatgtttg gccctttatc acagttcccc aggcagcaag ttggcagcag cgtaaagcga
    30301 ccgtcaaatg tcgtttgccc tggtttgatg ttcagctttt tctcgagcgc cgcctgaata
    30361 ccctggtaac cgttgatatg acagaccacg ctgtcgcaat aacggatcac atggcggcct
    30421 actggctgac ggaagatctg gctgtagaac gttgccacgc cttcgacgtc gctcgccgga
    30481 atacccagca catcggcgat cgcgtggatc gcgccatccg gcacccagcc acgctgcttc
    30541 tgaacgattt tcagcgcttc aatggacgcc gcacgcgggt cttcgtagtg gtgcatctcg
    30601 tgctcaatcg cttcacgctc tgccgcactc agctcaaaag cctcggtttg tggttgttga
    30661 ttctcgtgca taattagcgg tccacatctg acataacaaa atcgatactg cccagataaa
    30721 caatcaggtc agacaccagg ctgccgcgga tcgccgccgg aatttgctgc aaatgcgcat
    30781 aactcggcgt acggatacgg gtgcgataac tcatggtgct gccgtcgctg gtcaggtagt
    30841 aactgttgat ccccttggtc gcctcaacca tctggaagga ttcattggca ggcatcaccg
    30901 gaccccacga cacttgcagg aagtgggtga tcagggtttc gatatgttgc agcgtgcgct
    30961 ctttcggcgg cggcgtggtc agcgggtgat ccgctttgaa cgggccttcc ggcatgttgt
    31021 tgaggcactg ctcaagaata cgcagactct ggcgcaactc ttctactttc agcattacgc
    31081 gggtgtagca gtcagaaaca ccaccgccca ccggaatttc gaagtcgaag ttttcatagc
    31141 cagaataagg acgcgcctta cgcacgtcga agtcgatccc ggtggcacgc aggcccgcgc
    31201 cggtggtgcc ccactccagc gcctctttcg cgccgtaagc ggcaacgccc tgggaacgac
    31261 ctttcagaat ggtgttttgc agcgccgctt tctcgtaaga cgccagacgt ttcggcatcc
    31321 agtcgaggaa ctcacgcagc aggcgatccc agccgcgcgg caagtcgtgc gctacgccgc
    31381 caatacggaa ccacgccggg tgcatacgga aaccagtgat tgcttccacc agatcgtaaa
    31441 ttttctgacg atcggtaaag gcgaagaaca ctggcgtcat cgcgccgacg tcctgaataa
    31501 aggtggagat atacagcagg tgactgttga tgcggaacag ttcggagagc attacgcgaa
    31561 tgacgttaac gcgatccggc acggtgatcc cagccagttt ctctaccgcc agcacataag
    31621 gcatttcgtt aacgcagccg ccgaggtatt cgatacggtc ggtatacgga atgtagctgt
    31681 gccaggactg gcgttcgccc attttctcag cgccacggtg gtggtaaccg atgtctggta
    31741 cgcagtcgac aatctcttcg ccatcaagct gcaaaacgat acggaaagca ccgtgcgcag
    31801 acgggtggtt cggaccgagg ttgaggaaca tgaagtcctc attttcggtg ccgcgcttca
    31861 tcccccactc ttccggtttg aaggtcaggg cttccatctc cagatcctgt ttggctttgg
    31921 tcagctcaaa cggcgagaat tcggtagcgc gcgccgggta atctttacgc agcgggtgac
    31981 ctttccaggt ttgcggcatc atgatgcggc gcaggttcgg gtgaccgtcg aatgtaatgc
    32041 caaatagatc ccaggtttca cgctcatacc agttagcgtt cgggaataat ttggtaaagg
    32101 tcggtacgtg caggtcgttt tctgccagcg ccaccttcag catgatgtcg cggttacgat
    32161 cgatagaaat cagatggtag aaaacggaaa aatccgcggc aggtaaccct tcgcggtgtg
    32221 tgcgcagacg ttcgtccatg ccgtgtaagt caaacagcat gacgtaaggt ttcggcagtt
    32281 tctttaagaa atcgcccact tccagtaatt gttcacgctt gatccacaca acgggaaccc
    32341 cggtgcgagt cgcctgaaca gtaaaggcat ccggcccaaa acggttgcgc agttcgccaa
    32401 tcaccggatc atcaagatga tcgcgggtct gccaggcggg ttcttgcgcg gttaagtcgg
    32461 tcatattgtt caccattgca aatggtccgt ggtgactgtc ggcgcagtaa gcttcgcgaa
    32521 atagatttag gaataagcga agaaaattcc ctttgccgac aggcgcaaat taaatctcgt
    32581 caggtgtacg caggttggta acggcaatgc gttcaccgcg cttgcgttcg cgctctgatt
    32641 gcatattggc gcgataaacg ccctgatcgc caaccaccca ggagagcgga cgacgttctt
    32701 taccgataga ttcctgcaac agcatcagtg cctgcatgta cgcttcagga cgcggcggac
    32761 agcccgggat atacacatca accgggatga atttatcaac gccctgcaca acggaataaa
    32821 tatcgtacat accgccggag ttggcacagg cacccattga gataacccat tttggttcca
    32881 gcatctggtc atacaaacgc tgaataaccg gtgccatttt ggtaaagcag gttcctgcaa
    32941 ccaccatcag gtcagcctga cgcggcgaag cacgcaatac ttcagcgcca aaacgcgcca
    33001 cgtcatgtac cgcggtaaac gaagtcacca tctcaacgta acagcaggaa agaccgaagt
    33061 tatacggcca aattgagttt ttacgacccc agttaaccat gtcattgagc ttgcccataa
    33121 acacgttttt gttaacttct tgctccagag ggtcggttac gatctcctgc ttttgcaggg
    33181 ggtaacggtc attctcaccg ttgggatcta tgcgggtgag cgtataatcc atcttaatgc
    33241 ctcgcggtta gcgttgacga ttagcgatac tgttcgtttc cgggttcata cgctcgcggc
    33301 gtgaacgcgc gggcgtccag tccagcgcgc caatacgcac cagataaacc agacctgcca
    33361 gtaacacaaa aataaaaatt gcagcttcca caaagcctac ccagccgctt tcgcggatag
    33421 aggttgacca tgcgaacaga tacagcgctt caacgtcgaa gataacgaag aacatggcca
    33481 ccagataaaa cttggcggac aggcgtaagc gggcggagcc gaccgagtcg ataccggatt
    33541 caaacggaac gttttttgac ctcgcgcgtg cgcgaccgcc taaaaaccaa ccgcctacca
    33601 gcatcaggca acacaggcca atggcaacga taagaaagat agcgaatgcc cagtgatgag
    33661 cgatgacttc agtggatgtt gacatactca ttgcttactc atcaaaagta gcgcctgatt
    33721 ctctgctctt tacggcagat ggacgccaca tcgattcatg gggaggaata aaaaaaacct
    33781 tacaataact gcagaaacta ttttatacag ctaattgatg tggtctttta ctcctttcta
    33841 taaccttttg tcaactttaa caaaggtttc ttcacactga tttacacaat gacaacaaca
    33901 tttgcatttg gcgccctttc tccccagatc attgacgact ccaggaaaat tagatgttgt
    33961 tgaatcgtgt ccgttgtgaa gcaatggaaa aaatacgggt ctattttgac aggaatttgt
    34021 gtcgattcct ccccccaaaa gagagtattt tcttgatctg tgacacgctt ttgtcattct
    34081 ataacaaaaa tgcagcaaca aattcacgta ttttttaaca ttattgtagc aggagatttt
    34141 tttcaggtga ttatttgtgc gtacggggcg tgaatctctg gtgattgaaa aatgaacagt
    34201 tttgtacgtt ctgcacgata cgaggaaggc tttttactaa aaaaaagccg ctggggttta
    34261 aaataaaccc agcggttcgt ttttacacta ttgtctcagg aattatctat cgtccgtcga
    34321 ttactcgata tccctttcaa tcaacaatga gtcatctcct tccggagcag acattgggct
    34381 gtattgccac ggattatggt agctctccat cgcctgatag atcacctggg ccagctcatt
    34441 attactggac gggtcatagc acagcaaata ttcggtatca ggtagcggcg gtaagccatc
    34501 tactccactc agcacacgca ggtccggact catcatctcg accggccttg ccgtcacgcc
    34561 aagaccggct ttcactgccg cacgaacggc cggaagcgtc gaggcgacat aagccagtcg
    34621 ccagggaata tctgctttat taagcgtcgc cagcaccata tcgcgaaacg gacttggatc
    34681 atccagcaac acaagaggga tcggctcacc tttttgcaat acgtattccg ctgcgcagta
    34741 ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaag gtagaaggac gatgggtggt
    34801 taccatcaaa tccacttcct gggattccag catttctgcc atgtaggcat tacgtttaac
    34861 gcggacatcc agcgctagtt tcggataaac cgaactcacg cgatttaaca ggaaaggtaa
    34921 gatcgtatcg gcagattcat ctgaagcacc gatagttaac acgccctgaa gattactgaa
    34981 cattaatgat gagcaggcct catcattaaa acgcaggatt ttcctggcgt aaccaagaag
    35041 ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca gttctttccc
    35101 aacgagttgt tccagacgct gcatttgctg acttacggcg gactgagtac gacacacagc
    35161 ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca acaaatgttc tcagcagatc
    35221 gaggtcgagg ttaattatcg gacgatttgc acttatcata tattatcact tactggcggc
    35281 tcatactgag ctggttaatg ctgtgcacac acaaacaagc aattccattt gtaatgtgcc
    35341 tccctggcag tttcatccgg accccggacg aaagtaaaaa tgcatatgag ttgcactaaa
    35401 aaagtgactc acattgttcc gttataatgc ctgaagtaga tcacagaata tatcttcagg
    35461 gatcgcatat ctattaagtt actcactctt ttctatttat gacatgcgcg tgtttgtata
    35521 aatgtaaatg tgagtccttg ttccactctc gtacagcatc gctggtcata cgcgaacacg
    35581 tacaaacagc aatggtgagg cggcatcaag agcagggatc cgtcatttat gcaagcattt
    35641 taccccaaaa accttttatt tataagggtc attgcgaatt atctgatgca
//
LOCUS       DAZFJL010000031        35596 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFJL010000031 DAZFJL010000000
VERSION     DAZFJL010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.031"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            117..917
                     /gene="ydcF"
                     /locus_tag="ACHJ8C_001553"
     CDS             117..917
                     /gene="ydcF"
                     /locus_tag="ACHJ8C_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO5551431.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRITGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            1114..2563
                     /gene="aldA"
                     /locus_tag="ACHJ8C_001554"
                     /pseudo
     CDS             1114..2563
                     /gene="aldA"
                     /locus_tag="ACHJ8C_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(2605..3606)
                     /gene="gap"
                     /locus_tag="ACHJ8C_001555"
     CDS             complement(2605..3606)
                     /gene="gap"
                     /locus_tag="ACHJ8C_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO5551432.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDYTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKKATNNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            3795..4325
                     /gene="cybB"
                     /locus_tag="ACHJ8C_001556"
     CDS             3795..4325
                     /gene="cybB"
                     /locus_tag="ACHJ8C_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO5551433.1"
                     /translation="MENKYSRLQISIHWLVFLLVIVAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPITPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4570..4743
                     /locus_tag="ACHJ8C_001557"
     CDS             4570..4743
                     /locus_tag="ACHJ8C_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5551434.1"
                     /translation="MKKLALILVMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            4769..4837
                     /gene="yncP"
                     /locus_tag="ACHJ8C_001558"
     CDS             4769..4837
                     /gene="yncP"
                     /locus_tag="ACHJ8C_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO5551435.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(4815..4964)
                     /gene="hokB"
                     /locus_tag="ACHJ8C_001559"
     CDS             complement(4815..4964)
                     /gene="hokB"
                     /locus_tag="ACHJ8C_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO5551436.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            5354..6994
                     /gene="trg"
                     /locus_tag="ACHJ8C_001560"
     CDS             5354..6994
                     /gene="trg"
                     /locus_tag="ACHJ8C_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO5551437.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRSVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAEQAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQMVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIESLISESVRLIDLGSDEVATAGKTM
                     CTIVDAVASVTHIMQEITIASDEQSRGITQVSQAISEMDKVTQQNASLVEEASTAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(7032..7955)
                     /gene="ydcI"
                     /locus_tag="ACHJ8C_001561"
     CDS             complement(7032..7955)
                     /gene="ydcI"
                     /locus_tag="ACHJ8C_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5551438.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMNGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            8172..9515
                     /gene="ydcJ"
                     /locus_tag="ACHJ8C_001562"
     CDS             8172..9515
                     /gene="ydcJ"
                     /locus_tag="ACHJ8C_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO5551439.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKVLIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLGWFRYRLTPSGEAHRQAINPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETRACNHGDASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            9740..11395
                     /gene="opgD"
                     /locus_tag="ACHJ8C_001563"
                     /gene_synonym="mdoD"
     CDS             9740..11395
                     /gene="opgD"
                     /locus_tag="ACHJ8C_001563"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO5551440.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKNQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            11535..11759
                     /gene="ydcH"
                     /locus_tag="ACHJ8C_001564"
     CDS             11535..11759
                     /gene="ydcH"
                     /locus_tag="ACHJ8C_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO5551441.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQVSVKEV"
     gene            11822..12361
                     /gene="rimL"
                     /locus_tag="ACHJ8C_001565"
     CDS             11822..12361
                     /gene="rimL"
                     /locus_tag="ACHJ8C_001565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO5551442.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(12353..13333)
                     /gene="ydcK"
                     /locus_tag="ACHJ8C_001566"
     CDS             complement(12353..13333)
                     /gene="ydcK"
                     /locus_tag="ACHJ8C_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO5551443.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAMSDFNDVIAGTAG
                     GWIDREMVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASIEGHEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTIHIRGPKVINGEERITRTLLAGLL"
     gene            13457..14449
                     /gene="tehA"
                     /locus_tag="ACHJ8C_001567"
     CDS             13457..14449
                     /gene="tehA"
                     /locus_tag="ACHJ8C_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO5551444.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTGAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            14446..15039
                     /gene="tehB"
                     /locus_tag="ACHJ8C_001568"
     CDS             14446..15039
                     /gene="tehB"
                     /locus_tag="ACHJ8C_001568"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO5551445.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLITNMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            15342..16010
                     /gene="ydcL"
                     /locus_tag="ACHJ8C_001569"
     CDS             15342..16010
                     /gene="ydcL"
                     /locus_tag="ACHJ8C_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO5551446.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(16045..17256)
                     /gene="ydcO"
                     /locus_tag="ACHJ8C_001570"
     CDS             complement(16045..17256)
                     /gene="ydcO"
                     /locus_tag="ACHJ8C_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO5551447.1"
                     /translation="MLRYLYVITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLALTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNVLIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLAWLATRAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLFGIGSAFWGLIAGGVCY
                     VVLNLIANRNR"
     gene            17309..17845
                     /gene="sutR"
                     /locus_tag="ACHJ8C_001571"
     CDS             17309..17845
                     /gene="sutR"
                     /locus_tag="ACHJ8C_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO5551448.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSSTPSVYDPQRQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGVRFAADVTHVYR
                     NGGEQTVHFHSLIHYPRS"
     gene            17918..19879
                     /gene="rlhA"
                     /locus_tag="ACHJ8C_001572"
     CDS             17918..19879
                     /gene="rlhA"
                     /locus_tag="ACHJ8C_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO5551449.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHSYGAKIFVTLNTILHDDELEPAQRLITDLYQAGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHIDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPAELHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARNVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDDLLKTLPKRKG"
     gene            complement(19971..20201)
                     /gene="yncJ"
                     /locus_tag="ACHJ8C_001573"
     CDS             complement(19971..20201)
                     /gene="yncJ"
                     /locus_tag="ACHJ8C_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
                     /protein_id="HFO5551450.1"
                     /translation="MFSKALSVVLLTCALFSGQLMAGHKGHEFMWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            20380..20616
                     /gene="hicB"
                     /locus_tag="ACHJ8C_001574"
                     /pseudo
     CDS             20380..20616
                     /gene="hicB"
                     /locus_tag="ACHJ8C_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
     gene            20695..22101
                     /gene="ydcR"
                     /locus_tag="ACHJ8C_001575"
     CDS             20695..22101
                     /gene="ydcR"
                     /locus_tag="ACHJ8C_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO5551451.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKATVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNVELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKVRLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLVALTHHISIAPGNMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            22346..23491
                     /gene="ydcS"
                     /locus_tag="ACHJ8C_001576"
     CDS             22346..23491
                     /gene="ydcS"
                     /locus_tag="ACHJ8C_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO5551452.1"
                     /translation="MSKIFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKALDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     NSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            23509..24522
                     /gene="ydcT"
                     /locus_tag="ACHJ8C_001577"
     CDS             23509..24522
                     /gene="ydcT"
                     /locus_tag="ACHJ8C_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5551453.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGTPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGEMQANGT
                     IQAVQYQGAATRFELTLSGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVALV
                     EER"
     gene            24523..25464
                     /gene="ydcU"
                     /locus_tag="ACHJ8C_001578"
     CDS             24523..25464
                     /gene="ydcU"
                     /locus_tag="ACHJ8C_001578"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5551454.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIILIALYLAFVKRLGAFDAL"
     gene            25454..26248
                     /gene="ydcV"
                     /locus_tag="ACHJ8C_001579"
     CDS             25454..26248
                     /gene="ydcV"
                     /locus_tag="ACHJ8C_001579"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5551455.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            26270..27694
                     /gene="patD"
                     /locus_tag="ACHJ8C_001580"
     CDS             26270..27694
                     /gene="patD"
                     /locus_tag="ACHJ8C_001580"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO5551456.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGSPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(27791..27886)
                     /gene="yncL"
                     /locus_tag="ACHJ8C_001581"
     CDS             complement(27791..27886)
                     /gene="yncL"
                     /locus_tag="ACHJ8C_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO5551457.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            28080..28253
                     /gene="ortT"
                     /locus_tag="ACHJ8C_001582"
     CDS             28080..28253
                     /gene="ortT"
                     /locus_tag="ACHJ8C_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO5551458.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            28339..28572
                     /gene="ydcY"
                     /locus_tag="ACHJ8C_001583"
     CDS             28339..28572
                     /gene="ydcY"
                     /locus_tag="ACHJ8C_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO5551459.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREERYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGIIL"
     gene            complement(28573..29022)
                     /gene="ydcZ"
                     /locus_tag="ACHJ8C_001584"
     CDS             complement(28573..29022)
                     /gene="ydcZ"
                     /locus_tag="ACHJ8C_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO5551460.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSNVRWWTLIPGLLGSFFVFASISGYQNVGAAITIAV
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRTF"
     gene            complement(29019..29537)
                     /gene="mddA"
                     /locus_tag="ACHJ8C_001585"
     CDS             complement(29019..29537)
                     /gene="mddA"
                     /locus_tag="ACHJ8C_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO5551461.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEENGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            29786..29941
                     /locus_tag="ACHJ8C_001586"
                     /pseudo
     CDS             29786..29941
                     /locus_tag="ACHJ8C_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(30018..32120)
                     /gene="pqqU"
                     /locus_tag="ACHJ8C_001587"
     CDS             complement(30018..32120)
                     /gene="pqqU"
                     /locus_tag="ACHJ8C_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO5551462.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTEAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNKFRLNRGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMAFASIGYIPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGINIAWRFE"
     gene            32362..33423
                     /gene="yncE"
                     /locus_tag="ACHJ8C_001588"
     CDS             32362..33423
                     /gene="yncE"
                     /locus_tag="ACHJ8C_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO5551463.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTTRQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(33536..35035)
                     /gene="ansP"
                     /locus_tag="ACHJ8C_001589"
     CDS             complement(33536..35035)
                     /gene="ansP"
                     /locus_tag="ACHJ8C_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO5551464.1"
                     /translation="MSKHNTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETSN"
     gene            35233..35331
                     /locus_tag="ACHJ8C_001590"
                     /pseudo
     CDS             35233..35331
                     /locus_tag="ACHJ8C_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233886.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
BASE COUNT         9054 a         8622 c         9078 g         8842 t
ORIGIN      
        1 aaatatgtca ggcttgcgga gtggcagtta aggaaatacg acttcctcct ttcagagtgc
       61 tccgcttctc actattatct cacgcagtat tcttaaggga acgataagga ggtaccatga
      121 atattacccc gtttccgacg ctttcgccgg caaccataga tgccataaat gttatcggac
      181 agtggctggc gcaggatgat ttctccggtg aggtgccgta tcaggccgat tgcgtgatcc
      241 ttgcaggcaa tgcggttatg ccgactatcg atgcggcatg taagattgcc cgcgatcagc
      301 aaattccttt actgattagt ggtggtatcg gtcactcgac aacttttttg tatagcgcca
      361 tcgcacagca tccgcactac aacactatcc gcaccactgg cagagcagaa gctaccattc
      421 tggcggatat cgctcatcag ttctggcata ttccgcatga aaaaatctgg attgaagacc
      481 agtcaacaaa ttgcggtgaa aacgcacgct ttagcatcgc gctattgaat caggccgtag
      541 aacgagttca tacggctatc gttgttcagg accccaccat gcagcggcgc actatggcga
      601 cgttccgccg tataaccggg gataatcccg atgcaccacg ctggttaagt tatcccggat
      661 tcgttcctca gttaggaaat aacgcagaca gtgtaatctt tgttaatccg ttgcaaggat
      721 tatggccggt tgagcgttat ctatcgctac tcactggcga gttgccgcgt ttacgcgatg
      781 atagcgatgg ctacggcccc cgcgggcgag attttatcgt tcacgttgat tttccggcag
      841 aagtcattca ggcatggcaa acgctgaaac atgatgcggt gctcatcgag gcgatggaaa
      901 gtcgctcgtt acgttaaaaa ttgcccgttt gtgaaccacc tgtttgcaaa cgggcatgac
      961 ttctgacttt tatttctgcc tttaattcct tttacacttg tttttatgaa gcccttcaca
     1021 gaattgtcct ttcacgatgc cgtctctctg ataattgatg ttaattaaca ataaatttac
     1081 caaaaacaaa catgtaaatc acaggagtcg cccatgtcag tacccgttca acatcctatg
     1141 tatatcgatg gacagtttgt tacctgtcgt ggagacgcat ggattgatgt ggtaaaccct
     1201 gctacagagg ctgtcatttc ccgcattccc gatggtcagg ccgaggatgc ccgtaaggca
     1261 atcgatgcag cagaacgtgc acaaccagaa tgggaagcgt tgcctgctat tgaacgcgcc
     1321 agttggttgc gcaaaatctc cgccgggatc cgggaacgtg ccagtgaaat cagtgcgctg
     1381 attgtcgaag aagggggcaa gatccagcag ttggctgaag tcgaagtggc ttttactgcg
     1441 gactatatcg attatatggc ggagtgggca cggcgttacg agggcgagat tattcaaagc
     1501 gatcgtccag gagaaaatat ccttctgttt aaacgtgcgc ttggcgtgac taccggcatt
     1561 ctgccgtgga actttccgtt cttcctcatt gcccgcaaaa tggctcccgc tcttttgacc
     1621 ggtaatacca tcgtcattaa acccagtgaa tttacgccaa acaatgcgat tgcattcgcc
     1681 aaaatcgttg atgaaatagg ccttccgcgc ggcgtgttta accttgtact cgggcgtggt
     1741 gaaaccgttg ggcaagaact ggcgggtaac ccaaaggtcg caatggtcag tatgacaggc
     1801 agcgtctctg caggtgaaaa gattatggcg actgcggcga aaaacatcac caaagtgtgc
     1861 ctggaattgg ggggtaaagc accggctatc gtaatggacg atgccgatat tgaactggca
     1921 gtcaaagcca tcgttgattc acgcgtcatt aatagtgggc aagtgtgtaa ctgtgcagaa
     1981 cgtgtttatg tacagaaaag catttatgat cagttcgtca atcggctggg ggaagcgatg
     2041 caggcggttc aatttggtaa ccccgctgaa cgcaacgaca ttgcgatggg accgttgatt
     2101 aacgccgcgg cgctggaaag ggttgagcaa aaagtggcgc gcgcagtaga agaaggggcg
     2161 agagtgggcg agagtggcgt tgggtggcaa agcggtagag gggaaaggat attattatcc
     2221 gccgacattg ctgctggatg ttcgccagga aatgtcgatt atgcatgagg aaacctttgg
     2281 cccggttctg ccggtggtcg catttgacac gctggaagag gctatctcaa tggctaatga
     2341 cagtgattac ggcctgacct catcaatcta taccaaaaat ctgaacgtcg cgatgaaagc
     2401 cattaaaggg ctgaagtttg gtgaaactta catcaaccgt gaaaacttcg aagctatgca
     2461 aggcttccac gctggatggc gtaaatccgg tattggcggc gcagatggta aacatggctt
     2521 gcatgaatat ctgcagaccc aggtggttta tttacagtcg taatgagtga aagaggcgga
     2581 ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat ttttcgaggg tgcgaataag
     2641 ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc gttttcacca gttgtaaatc
     2701 gcccacggcg gtaatttccg tttgcgtggc atcaaacacc gaaccgaaat ggctgccaat
     2761 gatatcggaa gagactattt cttcatcggt ataaccaaat gactcgttat tgtttgtcgc
     2821 ctttttaagc gcgttgttca cctcttcggc agtcactttc tttccgagaa tcgacaccag
     2881 ttcagtgacc gaacctgttt tcaccggcac gcgttgcgca tgacctttca gtttgccgct
     2941 cagttccggg atcaccagac caatggcttt tgccgccccc gtagtgtggg gaatgatatt
     3001 ttctgccgct gcgcgtgaag cacgtaaatc tttaccacgt gggccatcca ccagtgactg
     3061 ggtgccggta taggcatgaa tggttgtcat cgtgccgact tctatcccga aactgtcatg
     3121 caaggctttg gccatcggcg caaggcagtt agtggtgcat gacgccacgg aaacaatggt
     3181 gtcgttgcca tccagagtgt cgtcattgac gttataaacg atggttttca tttcaccggc
     3241 aggggcggaa atcaacacct tcttcgcgcc agcatcaaga tgcgcctgcg atttctcggc
     3301 ggaggtataa aagcctgtac attcgacaat gatttctgcg cctttcgctt tccacggaat
     3361 atttttagcc tctttttcgg cgtaaaccgc gatacttttc ccatcaacga taagtgaatc
     3421 ttccgtataa tcaacgctcc aggggaacgg tccgtagttt gaatcatgtt tcagcaggta
     3481 ggcgagaatt tttggggaag tgagatcatt aatagcgaca acgtctatgt tgcttttgac
     3541 ttcaagtaat cgacgcaaca ccagtcgacc gatacgacca aaaccattaa taccaacttt
     3601 actcatggtc ttctcctgtc aggaacgttc ggatgaaaat tgatcctttc caggcttaga
     3661 ccaggatggc gggatgggca atctacattc ttgcagtgaa acgtaacgta ctgaaaacgg
     3721 gtgaacaata tttaatgaaa ttttaagaaa agcccgttat gttaacggaa aattatgtta
     3781 aagcaggaaa tgttatggaa aataaatact caaggttaca aatcagcatt cactggctgg
     3841 tctttttgct ggttatcgta gcgtattgcg caatggagtt tcgtggtttc ttcccacgta
     3901 gcgatcggcc cctcatcaac atgattcatg tttcctgtgg catctcaatt ctcgtactga
     3961 tggtggttcg tcttttgtta aggctgaaat acccaacccc gcctattaca cctaagccaa
     4021 aaccgatgat gacgggactg gcgcatttgg gccatttggt gatttatctc ctgtttattg
     4081 cgctgccagt gattggtttg gtgatgatgt ataaccgggg caacccgtgg tttgcgtttg
     4141 gtttgacgat gccttacgct tcagaggcca atttcgagcg ggttgatagc ttaaagtcgt
     4201 ggcatgaaac gctggcgaat ctgggatatt ttgtcatcgg gttgcacgct gcggctgcac
     4261 tggcacacca ctatttctgg aaggacaaca cacttctacg catgatgccg cgtaaacgtt
     4321 cctgaaggat atttaaagaa aacgcctgta ctaaaaccga cccgtggtac aggcgaagaa
     4381 tacgggccta catcggaagc gcctattata tttatttgta tgacaaataa aaccataatc
     4441 cttgtctata cgtccatctg gcttatcttt aatcaattca cccgatcttt gatctcatca
     4501 acggtatcaa aataaaccag cgtataacgt tcaaatcgaa cataagattc gataaatcat
     4561 ggaggttata tgaaaaaact ggcacttatc ctggttatgg gaacgcttgt ttccttttat
     4621 gccgatgccg ggcgcaaacc ctgttctggt tcgaaagggg ggatctcaca ctgtacggca
     4681 ggcggcaaat ttgtctgtaa tgatggttct attagtgcat cgaaaaaaac atgcactaac
     4741 tgaagtgtaa aaggggtgcc atgagaaaat gaatctgcta gtcagatgcg cggggaaaat
     4801 ccccgcgctt gcccttacct ggacgtgcag gccatgagcg cagcaacctc cttatcaccg
     4861 tcccggaacc gcagttcgta gagcgtttgt cgggtcagga gtgtgaatgt cagaatcgta
     4921 atgcagataa tgagcagaca caccaccaga gggttgcgct tcatagcctt tctccttgcc
     4981 ggatggcggg taagaggcta acatctgaat tgcttggttc attcgttggc ctcggttgat
     5041 agaaatatcg gtcggggcct tcgtctttct gattcccggt tagcctgaaa acagaaagtc
     5101 tcaggcaccc gcaggcatcc tatgaggttt ccttagggac gaaaataatc acttgacgaa
     5161 attgcgtgct gttttccaga atttttcgtc attcggttta gccgttcgtt actctcttca
     5221 ttccaatggc attattcttc tctgccataa ttgttgtaaa aatgtgacgc aaagaggttt
     5281 ttggtcataa gtaattaccg tcaagtgccg atgactttct atcaggagta aacctggacg
     5341 agagacaacg gtaatgaata caactccctc acagcgatta ggttttttgc atcacatcag
     5401 gctggttccg ttatttgcct gcattcttgg cggtatctta gttctattcg cattaagttc
     5461 tgctctggct ggctatttcc tctggcaggc cgatcgcgat cagcgtgatg ttactgcgga
     5521 gattgagatt cggaccgggt tagcgaacag ttcagatttt ttgcgttcag cccggatcaa
     5581 tatgattcag gccggggcag cgagtcgtat tgcggaaatg gaagcaatga agcgaaatat
     5641 tgcgcaagcc gaatcggaga ttaaacagtc gcagcaaggt tatcgtgctt atcagaatcg
     5701 atcggtgaaa acacctgctg atgaagccct cgacactgaa ttaaatcaac gcttccaggc
     5761 ttatatcacg ggtatgcaac ccatgctgaa atatgccaaa aatggcatgt ttgaggcgat
     5821 tatcaatcat gaaagtgagc agatccgacc gctggataat gcttataccg atattttgaa
     5881 caaagccgtt aagatacgta gcaccagagc caaccaactg gcggaacagg cccatcagcg
     5941 cacccgcctg ggtgggatgt tcatgattgg cgcgtttgtg cttgccctgg tcatgacgct
     6001 gataacattt atggtgctac gtcggatcgt cattcgtcca ctgcaacatg ccgcacaacg
     6061 gattgaaaaa attgccagtg gcgatctgac gatgaatgat gaaccggcgg gtcgtaatga
     6121 aatcggtcgc ttaagtcgtc atttacagca aatgcagcat tcactgggga tgacagtagg
     6181 gactgttcga cagggtgcgg aagagattta tcgtggcacc agcgaaattt cagctggcaa
     6241 tgcggacctg tcatctcgca ccgaagaaca agcggcggct atcgaacaaa ctgccgccag
     6301 catggagcaa ctcactgcga cggtgaaaca gaatgcggat aacgcgcatc atgccagcaa
     6361 actggcgcaa gaggcttcta ttaaagccag cgatggcggg cagatggttt ccggtgtagt
     6421 aaaaacgatg ggcgctatct ccacgagttc gaagaaaatt tctgagatca ccgccgtcat
     6481 caacagtatt gctttccaga cgaatattct ggcactgaat gctgccgttg aagccgcgcg
     6541 agcgggtgag caaggacgtg gatttgccgt tgtcgccagc gaagtacgga cactcgcaag
     6601 ccgcagcgct caggcggcga aagagattga aagcttgatc agtgaatcag tcaggttaat
     6661 tgacctggga tcggatgagg tggcaacggc agggaaaacc atgtgcacta ttgttgatgc
     6721 cgtcgcgagt gtcacacata tcatgcagga aatcaccatc gcctcggatg aacaaagtcg
     6781 aggcataacg caggttagcc aggcgatttc tgaaatggat aaggtgacgc aacagaatgc
     6841 ttctctggta gaagaggcct caacggcagc ggtgtccctt gaagaacagg cggcacgact
     6901 aactgaggcg gtggacgtat tccgtctgaa caaacattct gtgtcagcag aaccacgagg
     6961 agcgggtgaa ccagttagtt ttgctacggt gtgaaaatgt taaaggaggg atcgacagat
     7021 cccttcacct ttcagaacgg cattgatttt cgaatagcgt taatcatcaa ctggcaacca
     7081 gaagagaacg tcgcatctac gcgggtcagt attccaatcg gttcgcctgc accatgtccc
     7141 ggaacaggca gggctaccag cgtggcatga cgcaggtcgt cttttacagc gccagaaggg
     7201 acaaaccaca cgtaatcgta ttcaaccgta agttgacgag atagcgaagc agacagcgtt
     7261 tcgatacaac ccgaaggaat tttacatccc tggctctgca ctaatgcatc tgaatgctgg
     7321 cgtggcgcag tgccttctgg tgatacaacg accggccatt ccagcacccg gcttagcgtt
     7381 acgttctcct gaagtagcgg gtgattaggg cggacaacca gcttcagcga ttcaagaaac
     7441 agcagttcgt aattaagccc gttcatcagt tcaggatctg acatccgacc aatgccgata
     7501 tcgatttccc cggtttttaa acccgccaga atcatagggt tactcattgt cgcaacttgc
     7561 aaggtcgtct ctttttgttg ctgatgaaac tgacctataa ccgaaggtaa tatccctagt
     7621 gccgcagtag gtagcgcacc aaccctgacg acatcattat taagaccttc tttacgatga
     7681 agcgactgtc cggcagtgtt gatggcgtca agaactctga ctgcatgcgt taaaaattgt
     7741 tcgccgggta aggtaagttg cgccccctga cgaccacgct caaacaagcg agcgcccgtc
     7801 agctgctcca gttcattcaa tgtcttagag agcgcaggtt gactcaaatt aagggtttca
     7861 gccgcgcgcc ccaaagttcc ttgttgtgcg acagctacga atgtatgaag gtggcgcaaa
     7921 cggatgcgct gactaaacag actatttttt tccataagcg atgttaaaaa cgaagcggtg
     7981 tcgctgacaa gtgaagttgt tttattatga taacttgatt gcaaaatatt attaacaatt
     8041 aaagcaacta tgttacagca aaatggataa tattgatgtt ttcgcggcga gatcacagtt
     8101 tgtaaattct tccagccgga gtgaatgcgg ttacctacac tccagattac tgaccactgg
     8161 aggcagacac tatggcgaac agcatcacgg cggatgagat tcgggaacag ttttcgcagg
     8221 caatgtcagc catgtaccag caagaagttc cgcagtacgg cacgctgctg gaactggtag
     8281 ctgatgtaaa tctggcggtg ctggaaaaca atcctcaact gcacgaaaaa atggtaaatg
     8341 cagacgagct ggcgcgactg aatgttgaac gtcatggggc gattcgcgtt gggactgcac
     8401 aagagcttgc tactcttcgg cggatgtttg ccattatggg gatgtacccg gtgagctatt
     8461 acgatctctc gcaggcaggg gtgccggtac attcgacagc atttcggccc attgatgatg
     8521 cttctctggc gcgtaatccc ttccgcgttt ttacctcgtt actccgtctt gagcttatcg
     8581 agaacgaaat tttgcgccag aaagcggcgg agattctgcg tcagcgcgat atcttcaccc
     8641 cacgttgtcg acaactgtta gaggaatatg agcagcaggg cggttttaac gaaacacagg
     8701 cacaggagtt tgtgcaggaa gccctggaaa cgtttcgctg gcaccagtca gcaacggtag
     8761 atgaagaaac ctatcgcgcc ttgcacaacg aacatcggtt gattgctgat gtggtctgtt
     8821 ttcctggatg ccatatcaac catctgacgc cacgcacgct ggatattgac cgggtgcagt
     8881 cgatgatgcc tgaatgcgga attgaaccca aagttctgat cgaagggccg ccgcgccgcg
     8941 aggtgccgat tttactacgc cagaccagct ttaaagcact ggaagagacg gtgttgtttg
     9001 cggggcagaa acagggcacg cataccgcgc gctttggtga aattgagcag cgtggtgtgg
     9061 cattaacgcc gaaagggcga caactgtatg atgatcttct gcggaacgct ggaaccgggc
     9121 aggataatct cactcaccaa atgcatttac aggaaacctt ccgcactttt cctgacagtg
     9181 agtttttaat gcgtcagcaa gggctgggat ggttccggta ccgtctgacg ccttcgggtg
     9241 aggcgcatcg tcaggcgatt aatcccggag acgatccaca gcccttaata gaacgtggtt
     9301 gggtagtggc gcaacccatt acctatgaag atttcttgcc cgttagcgcg gcggggatct
     9361 tccagtcaaa tctgggtaat gaaacgcggg catgcaatca cggtgatgcc agtcgcgaag
     9421 catttgagca ggcgttgggg tgtccggttt tggatgagtt ccagctttat caggaagcgg
     9481 aagaacgcag taaacgtcgc tgtggtttgc tttaaattct gaccatccgc ctttgcaaaa
     9541 atttgcctga ttttacaaac gaatcaggct catcccatcg acataaaaaa aatgccaatt
     9601 tatgcatatt ctctgatttc aacaattgga ttattaataa atattgtcta gagtgagcgg
     9661 tcataaataa gcactttctt gccgctgaaa atgaccagcg cgggaccatt cacaacacca
     9721 gaaggactca ctttcaggta tggatcgtag acgatttatt aaaggttcaa tggctatggc
     9781 cgccgtgtgc ggtaccagcg gcattgcttc tcttttttct caggcggcat tcgcggccga
     9841 ttctgatatt gccgacgggc aaacccagcg ttttgacttc tccattctac agtcaatggc
     9901 gcacgactta gcgcaaacag catggcgtgg tgcaccacgt ccgttacctg atactctggc
     9961 gacaatgacg ccgcaggctt ataacagtat tcaatacgac gccgaaaaat cgctctggca
    10021 taacgttgaa aaccgtcaac tggacgctca gtttttccat atgggaatgg gattccgtcg
    10081 ccgcgttcgt atgttttctg tagatccagc aacacatctg gcgcgtgaaa ttcactttcg
    10141 cccggagttg ttcaaataca acgatgcggg tgtggatacc aaacaattag aagggcaaag
    10201 cgatctcggt tttgccggtt ttcgcgtgtt taaagccccc gaactggcgc gccgtgatgt
    10261 agtatcattc ctcggcgcga gttatttccg cgccgttgat gacacatatc aatacggttt
    10321 gtcggcccgc ggcctggcga tcgacactta caccgacagc aaagaagagt tccccgactt
    10381 taccgccttc tggtttgata cggtaaaacc aggggcaacc acctttaccg tttatgcgtt
    10441 gctcgatagc gccagcatta ctggtgccta taagttcact atccattgtg agaaaaatca
    10501 ggtgattatg gatgtggaaa atcacctgta tgcgcgcaaa gacattaaac agctgggcat
    10561 tgcaccgatg accagtatgt tcagctgcgg tactaatgaa cgtcggatgt gcgacaccat
    10621 tcatccgcaa atccatgact ctgatcgtct gtccatgtgg cggggcaacg gcgagtggat
    10681 ttgccgtccg ctgaacaatc cgcaaaaatt gcagttcaat gcttacaccg acaacaaccc
    10741 gaaagggttt ggtttattgc aactggatcg tgatttctcc cattatcagg acattatggg
    10801 ctggtataac aaacgcccaa gtctgtgggt ggaaccgcgt aacaagtggg gtaagggcac
    10861 catcggcctg atggaaatcc caacaacggg cgaaacgctg gataacattg tctgcttctg
    10921 gcagccagaa aaagctgtaa aagcgggtga tgagtttgca ttccagtatc gtctgtactg
    10981 gagtgcgcaa ccgcctgttc attgcccatt agcgcgcgtt atggcgacgc gtaccggcat
    11041 gggcggtttc ccggaaggtt gggcgccagg tgaacactat cccgaaaaat gggcgcgacg
    11101 ttttgccgtc gatttcgttg gtggtgatct gaaagctgcc gcgccaaaag gcattgagcc
    11161 ggtgattacg ctttccagtg gggaagcgaa gcaaatcgaa attctctata ttgaacccat
    11221 tgatggttat cgtattcagt ttgactggta tccgacttcg gactccactg atccggtcga
    11281 tatgcggatg tatctgcgtt gtcagggcga cgctatcagt gaaacatggc tgtatcagta
    11341 tttcccgcca gcgccggata aacgtcagta tgttgatgac cgcgtgatga gttaatcgtt
    11401 ttttcttcgg catcttcttc gggaggtgcc gtctggttaa acacgatccc gctcgcattt
    11461 ttccctaagt taaatgagta atctgatggt gtgtatttca gatacacctt gtcagccact
    11521 aacagggagt gcgtatgttt ccagaatacc gagatttaat atcccgtctg aaaaacgaaa
    11581 atcctcgctt tatgtccttg ttcgataaac acaataaact tgatcatgaa attgccagaa
    11641 aggaaggttc cgacggtcga gggtacaatg cggaagtggt ccgcatgaaa aaacaaaagc
    11701 tacagttaaa agatgagatg ctcaaaatcc tgcagcaggt gagcgtcaaa gaggtgtaaa
    11761 ctttcctaag ccgccagcca ggcggctttt ttaacagctg catggattga ctggagataa
    11821 gatgactgaa acgataaaag taagcgaatc acttgaatta catgctgttg cggaaagtca
    11881 tgtcacacct ctttatcagt taatctgtaa aaataaaacc tggttacagc agtcgctaaa
    11941 ctggccgcag tttgttcaaa gtgaagagga cacgcgaaaa acggtgcagg gtaatgtgat
    12001 gttgcatcaa cgcggctatg ccaaaatgtt catgattttc aaagaagatg aacttatcgg
    12061 cgttatctct tttaatcgta ttgaaccact gaataaaacc gctgaaatcg gctactggct
    12121 ggacgaatct catcaggggc aggggatcat ttctcaggcg ctgcaggcat tgattcatca
    12181 ttacgctcag tctggtgaac ttagacgctt cgtgatcaaa tgtcgggtgg acaatccgca
    12241 aagcaaccag gtcgctttgc gcaatggttt tatccttgag gggtgcctta aacaggctga
    12301 gttcctgaat gatgcctatg atgatgtgaa cttatacgcg cgtattatcg attcacaata
    12361 accctgccag cagcgttcgc gtaatgcgct cttcgccgtt aatgactttt ggaccacgga
    12421 tatgaatcgt gtcgccatca aaggcttcaa cgacggcgtg gtcggttaac tccacatggt
    12481 tttcaattat caccgcacct gtaatacggc tctcaccttg tattacaacg tgctcgtcga
    12541 gcagaatcgg tccgccacgt accacggcat ttccgccaat caagacatga tgttttaaca
    12601 cacaattacc ttccacaatg gcatattccg ccacctgtga actgtaatga atcgtcggaa
    12661 tggcatcttc ttctatacca gctttcacct gcgcgtgacc gtaaacttta gcgcaatcgc
    12721 atagccagac gttgttctct tcatgacctt caatactggc aaaatcaaaa acttcggcgc
    12781 gatgttcaat aaaagcatac cggacgacgg catcgccata aatttgtgcc tggtggacaa
    12841 tacgcgaggc gctaaccctc gcgcgatcat aaatttgtag caggagttga tggtcgggcg
    12901 ttaagccttg tgcggcgacg atcatagaat gttgatcaat taaggcgtga ccaaatattc
    12961 tacattgacc gcaaactaat gagtcacgaa tggtgacact gtcacttata tatgcaccct
    13021 ggctgatttc actattatcg atccagacat tatccgttgc gtaaacctct ccccacagca
    13081 cgctggtgcc ggtgatacgc gtattcccgg aaatcaccgt tccgccgaac gcaatagcat
    13141 tctggtcgta aatccagcaa tttccttcct gtgctaacac catttcgcga tcgatccagc
    13201 cgccagcggt tcctgctatc acatcgttaa aatcgctcat ggcaataatt tgccgtaata
    13261 acacactttt tttagtgcca tcttcctgat aactaaaagc tcgctgttct tcacttagac
    13321 gatatttacg catagcgttt tcccacaggt gacttactat aaccgtagca aattctgcgg
    13381 ctctggctat gctcgagaaa attccataaa atgcatttca aatatacttt ataaattaaa
    13441 caaaatgagt aagaagatgc agagcgataa agtgctcaat ttgccggcag gctactttgg
    13501 tattgtgttg gggacgatag ggatgggatt tgcctggcgc tatgccagcc aggtttggca
    13561 ggtcagccac tggttggggg atgggctggt gattctggcg atgatcatct ggggattatt
    13621 gactggcgca tttattaccc gactcatacg ctttccgcat agcgtgctgg cagaagtgcg
    13681 ccatccagtg ctgagcagtt ttgtgagttt gtttccggca actacgatgc tggtggcgat
    13741 tggttttgtt ccgtggtttc gcccactggc ggtgtgcctg ttcagttttg gtgtcgtggt
    13801 tcagttggct tatgccgcct ggcaaactgc gggattatgg cgcggatctc accctgaaga
    13861 agctaccacg cctggactgt atctgccgac agttgccaac aactttatca gcgcaatggc
    13921 ctgtggtgcg ttgggctaca ccgacgccgg tctggtgttt ttaggcgcag gcgttttctc
    13981 atggctaagc ctggaaccgg tgatcttgca gcgtctgcgc agttcaggag aattacccac
    14041 ggcactgcgg acatcactcg gcattcagct cgctcctgcg ctggtggcct gtagtgcctg
    14101 gctgagcgtc aacggcggcg agggtgacac gctggcgaaa atgcttttcg gttatggact
    14161 gctgcaactg ctgtttatgc tacgtctgat gccatggtat ctctcccagc catttaatgc
    14221 ttcattctgg agtttctcgt tcggtgtatc tgcactggca accaccggtt tgcatctggg
    14281 gagtggcagc gataatggat ttttccatac gctggcggtg ccgctgttta tctttaccaa
    14341 ttttattatt gcaatactgc tcatccgtac ttttgcgctt ctgatgcagg gaaaattgtt
    14401 agtcagaacc gagcgcgccg ttttaatgaa agcagaggac aaagaatgat cattcgtgat
    14461 gaaaactatt ttactgataa atatgaatta acccgcacac actctgaagt gctggaagcg
    14521 gtgaaagtgg ttaaaccggg taaaacgctg gatctgggct gtggcaatgg tcgtaacagt
    14581 ctttacctgg cagccaatgg ttatgatgtt gacgcatggg ataaaaatgc catgagtatc
    14641 gccaacgtcg agcgcattaa gtccattgaa aatctggata atttacacac ccgggtcgtt
    14701 gatctgaata acctcacatt tgatggacag tacgatttta ttctttcgac tgtggtgctg
    14761 atgttccttg aggctaaaac catcccaggg ctgattacca atatgcaacg ttgcactaaa
    14821 cctggcggtt acaacctgat tgtggcggcg atggataccg ctgattatcc atgtaccgtc
    14881 ggcttcccgt ttgccttcaa agagggggaa ttacgtcgat attacgaagg ctgggagagg
    14941 gtgaaataca atgaagacgt cggcgagctg caccgcaccg acgccaacgg taatcgtatt
    15001 aaactgcgtt tcgccacgat gctggcacgt aaaaaatgat ccggtaagaa caaagcgcgt
    15061 gaaaattccc ctcgctgaga tggtttactg ttcttctttt cggtaagcat attttttatc
    15121 gaagggatgt gaaattaatc acagtagtcg aagtttttta gccgcttaac ttactgaaat
    15181 ttaactactg atgattgact tagccccttt ttcggcattg actatgtcgt ctgaaaaggg
    15241 gctaaaaaat ttattttcac caacactttt tttgccacaa cacgaagcgg cgctttttgc
    15301 tataacttag aaagtaatat aatcatctca ggaaactatt catgcgtacc acatcatttg
    15361 cgaaagttgc agctttatgc ggcttgttgg ctctgtctgg ttgtgcctct aaaatcaccc
    15421 agccagataa atattctggt tttttaaaca attactctga tttaaaagaa acaacctcgg
    15481 caacaggtaa acctgtttta cgttgggtag acccgagttt tgatcaaagc aaatatgaca
    15541 gcatcgtctg gaacccaatc acttattatc cggtaccgaa accttctacc caggtagggc
    15601 agaaagttct ggataaaatt ttgaactata ccaacaccga aatgaaagaa gcgatagcgc
    15661 agcgtaaacc actggttacc accgctgggc cgcgtagtct gattttccgt ggggccatta
    15721 ccggtgtaga taccagtaaa gaagggctgc aattctatga agtggttcct gttgcattag
    15781 tggtagcggg gacgcaaatg gctacaggcc accgaaccat ggatactcgc ttgtattttg
    15841 aaggtgagct gattgatgca gcgactaata aaccggttat caaggtcgtt cgtcagggcg
    15901 aaggtaaaga cctgaataac gaaagtacgc caatggcttt cgaaaatatt aaacaagtta
    15961 ttgatgacat ggcgaccgat gccaccatgt ttgacgttaa caaaaagtag ttccagacgc
    16021 gccatcgtta gatggcgctt tttattatcg gtttctgtta gcgattaaat tcaacaccac
    16081 gtaacaaacg cctccggcaa ttaatcccca aaacgcagaa ccaatcccga acagcgtcaa
    16141 tccacttgcc gttaccagaa atgccaccac cgccgcgtcg cgctcacgct cattatgcag
    16201 cgcctgatac aaactgccgc caatggtact caacagcgca agacctgcca gcatctggat
    16261 ccaacttacg ggcagggcag ccatcatccc ggtaatggca ctaccaaaca gacctgcgat
    16321 caaataaaaa ataccagcaa cggcagcggc cagccaacgt tgatctttat ccggatgcgc
    16381 ttccgggctt tggcaaatag ccgcggtgat tgccgcaata ccgacggaat aaacgccgaa
    16441 aggggaaaaa acaagcgcca gcaatccagt aaacacaatc aacggcgaaa caggagccga
    16501 atacccggct gctttcattg ctgcgatacc cggtgcgttt tgcgatgcca tcgtcaccag
    16561 aaaaaggggg agtgcaacgc tcagactgtg agcaaacgaa aaatcagggg taatataagt
    16621 ggggagaacg ggtttaaaga caacatcagt tgtgacaacg tcaccttgcg cgataacgat
    16681 gacgatccca ataatcatcg cggcaattac cgcatagcgc ggcgcaacgg ccctggttgc
    16741 cagccatgcc agcaacatac tgccacacaa cgtaaattga ccatccagac tggcaaacgc
    16801 ctgtaaacca aaacgtaata aaatcccggc aagcattgcc gccgcaagcg agtgcggaat
    16861 aatgcgcatc agacgagcaa agagtcccgt tatgccgcag aggactatta acacgttggt
    16921 gacaataaaa acgccgatgg cttcgttaag tgttagtccc tgtaatccgg tgaccaacaa
    16981 agccgcacca ggcgttgacc atgcggtgag aacaggtacg cgataccata atgtcagagc
    17041 cagcgtactg acgcccattg ccagccccag cgccgtcatc cagccagaga tttgtgcagt
    17101 ggtggctccg gcgacaatcg ctgcttgcca gattattgcc gctgaactgg cgtagccaat
    17161 taataccgcc agaaaccccg ccagtagcgt gggtggagga atagaaaaca gacgcatagt
    17221 cacctcgtgc gttataacgt acaggtaccg tagcatttgt ccgttataac gcacaagtga
    17281 taaacttccg ttttgccgga ggagtcgcat ggaaaatctc gctcgctttt tatccaccac
    17341 acttaaacaa ctacgtcagc agcgcggctg gagtctttcg cgactggcag aagcgacggg
    17401 cgtttctaaa gcgatgttag ggcagattga gcgtaatgaa tccagcccga cggtcgcgac
    17461 attatggaaa attgccaccg ggttgaacgt gccattttca acatttattt ctccgccgca
    17521 gtcctctacg ccttcagttt atgatccaca acggcaggcg atggtgatca cttcgctgtt
    17581 tccttatgat ccacaactct gctttgaaca cttctcgatt cagatggcac ccggtgcaat
    17641 aagcgaatcg acgccccatg aaaagggggt aatcgaacat gtcgtcgtca ttgatggaca
    17701 acttgatctg tgtgttgatg gtgaatggca gagccttaat tgtggggaag gcgttcgatt
    17761 tgctgcagac gtcacgcatg tctatcgtaa tggtggggag caaaccgtac attttcattc
    17821 cctcatccat tacccgcgca gttaagcggg aaaactattt cgcaacgtcg cgcttgtggc
    17881 taaaatagcc gccatttttc agctactgga taagaaagtg accgtatctt ctcatcgtct
    17941 tgaactgtta agcccggcac gcgatgccgc cattgcccgc gaagctattt tgcacggtgc
    18001 cgatgctgtt tatatcggcg gccctggttt tggtgcccgt cataatgcca gtaatagcct
    18061 gaaagatatt gccgagttgg tgccgtttgc ccatagttat ggtgcaaaaa ttttcgtcac
    18121 gcttaacacc attttgcatg atgatgagct ggaacccgcg caacggctga ttactgacct
    18181 ctaccaggcc ggtgtcgatg cgctgattgt tcaggatatg gggattctgg aacttgatat
    18241 tccgccgatt gaactgcacg ccagtacgca gtgcgatatt cgtacagtag aaaaagcgaa
    18301 gttcctctct gatgttggct tcacgcagat tgtgctggcg cgagagctga atcttgagca
    18361 gatccgcgcg attcaccagg ccacggacgc gaccattgaa ttctttattc atggcgcatt
    18421 gtgcgtggcc tattcgggtc agtgctacat ttctcatgcg caaacagggc gtagcgccaa
    18481 ccgtggcgat tgctcgcagg cgtgccgttt gccatacaca ttgaaagacg atcaggggcg
    18541 ggtggtttcc tatgaaaaac atctgctgtc gatgaaagat aacgatcaga ctgccaacct
    18601 cggcgcgctg attgatgctg gcgtacgctc cttcaagatt gaagggcgtt acaaagatat
    18661 gagctacgtg aagaatatca ccgcccatta tcgtcagatg ctggatgcca ttattgaaga
    18721 acgtggcgat ctggcgcgcg cttcatcagg tcgtactgaa catttctttg ttccatcgac
    18781 ggaaaagact ttccaccgtg gtagcacaga ttattttgtg aatgcccgta aaggcgatat
    18841 tggcgcgttc gattcgccga aatttatcgg cctgccggtg ggcgaagtat tgaaagtagc
    18901 gaaagatcat attgatgttg ccgttaccga gccactggca aatggcgatg gcctgaacgt
    18961 gatgattaaa cgtgaagtcg tcggttttcg tgccaatacg gtagagaaaa ccggagaaaa
    19021 tcagtaccgc gtctggccca atgaaatgcc agcagagttg cacaaaattc gcccgcatca
    19081 tccactaaac cgaaatcttg atcataactg gcagcaggca ctgacaaaaa cctccagcga
    19141 acgccgggtg gcggtagaca ttgaactggg cggctggcag gaacaactga ttctgaccct
    19201 caccagtgaa gagggtgtca gcatcacaca tacgctggac gggcagttcg acgaagccaa
    19261 taacgccgaa aaagcaatga acaatctgaa ggatggtctg gcaaaactgg ggcaaaccat
    19321 ctattacgcc cgcaatgtgc aaattaattt gccgggggcg ctgtttgtac caaacagtct
    19381 gttaaaccag ttccgccgtg aagccgccga tatgcttgat gctgcgcgtc ttgccagtta
    19441 ccagcgcggc agccgtaaac cggttgctga tcctgcgccg gtttatccgc aaacgcatct
    19501 gagtttcctc gcgaacgtat acaaccagaa agcgcgtgaa ttttatcatc gctatggtgt
    19561 gcaactgatt gacgcggcgt atgaggcaca tgaagagaag ggcgaagtcc cggtgatgat
    19621 caccaagcat tgtctgcgct ttgccttcaa tctgtgcccg aaacaggcga aaggcaatat
    19681 caaaagctgg aaggcgacgc caatgcaact ggttaacggc gatgaagtat taacgctaaa
    19741 gtttgattgc cgcccatgcg agatgcacgt cattggcaaa atcaaaaatc acatactgaa
    19801 aatgccgtta ccgggaagcg tagtggcatc cgtaagtccg gatgatctgc tgaaaacatt
    19861 gccgaagcga aaagggtaaa acgccagttt tcgggttact caccactcat caaatttgca
    19921 tgatattgcc tgccgggtaa ggcgtcatgc cgcatccggc aacaaatgac tcaacgctgt
    19981 cccgcttctg gtttgcgcga tttttgccag taaaaatgct cgcgcaaacc ttccgccgac
    20041 tcttccgcca cagcacgcaa ttcatcgctg tccgcttcat gacgcagctg atgatccaca
    20101 ttctttaccc acataaattc atgtcctttg tgccctgcca tgagttgtcc tgagaacagc
    20161 gcacacgtta ataagacaac cgataacgcc ttcgaaaaca tcccgccacc tttttctaac
    20221 cttttgccgc tatgatgccg atcgtttctt gaggttatta ttcagttttg caaattagag
    20281 caaagaaatt ctggaatctt ccttcctgat tttgcattgc attctgccgt tgcggcgatt
    20341 tagtgctatt ttcgagcaca ttacacacgg aggtaaactt cgccattaaa tagtcacgat
    20401 cactttattg aagtgccttt gagcgtcgcc tctaaggtat tgctgttaaa tgctttttta
    20461 cagtcagaaa tcactcagca agagttagcc aggcgaattg gcaaacataa acaggagatt
    20521 actcgcctat ttaatttgca ccatgtgaca aaaatcgacg ccgtccagct cgcggctaat
    20581 gcgcttggca aagagttatc gctggtgatg gtttgattac agttaatgaa aagttgtcat
    20641 ttttaacaac tgatatagac tgccgaatca tctgcacata attacgattc gataatgaaa
    20701 aaataccagc agcttgcaga acaattacgc gagcagattg cgtcgggtat ctggcaaccc
    20761 ggcgatcgtt tgccttcgtt gcgtgaccag gtggcgcttt ctggcatgag ctttatgact
    20821 gtcagccatg cctatcagtt gctcgaaagt cagggatata ttatcgcgcg accgcaatcg
    20881 ggttattacg ttgcgccaca ggcaataaaa atgccgaaag cgacagtcat tccagtcaca
    20941 cgagatgaag cagtcgatat caacacctat atttttgata tgttgcaggc cagtcgtgat
    21001 ccgtcggtag ttccgtttgc ctcggccttt cccgacccgc gacttttccc cctccaacaa
    21061 ctaaaccgct cgctggcgca ggtaagcaaa accgccacgg cgatgagcgt gattgaaaac
    21121 ttaccgccag gaaacgtaga actgcgtcag gctattgctc gtcgctatgc cttacagggc
    21181 atcaccattt ctcctgatga aattgtcatt accgccgggg cgttagaggc attaaacctc
    21241 agtttgcaag cggtaactga accgggcgat tgggtgatag tagagaatcc ttgtttctac
    21301 ggcgcgttgc aggcgctgga gcggttacgg ctgaaggcgt tatcggtggc gacggatgtt
    21361 aaagaaggga tagatcttca ggcgctggaa ctggcgttgc aggagtatcc ggtgaaagcg
    21421 tgctggctga tgactaatag ccagaaccca cttggattta ccttaacgcc gcagaaaaaa
    21481 gtgcggctgg tagcgttgct caatcagtac aacgtaacgc tgattgaaga tgacgtttat
    21541 agcgaacttt attttggacg ggaaaaaccg ctgcctgcga aagcgtggga tcgccacgat
    21601 ggtgttttgc attgctcttc gttttcgaaa tgtctggtgc ctggttttcg tattggttgg
    21661 gtcgccgccg gaaaacatgc acgtaaaatt caacgcttgc agttgatgag tacgctttcc
    21721 accagctcac cgatgcagct tgcgctggtg gattaccttt ctactcgccg atacgatgct
    21781 catcttcgtc gcctgcgtcg ccagcttgcg gaacgtaaac aacgcgcctg gcaggctttg
    21841 ctgcgttatc tgcctgcgga agtcaaaatt catcacaatg acagcggtta ttttctctgg
    21901 ctggagctcc ccgagccgtt agatgcagga gaattaagcc tggtggcact gacgcatcat
    21961 atcagtattg cgccgggtaa tatgttttct accggtgaaa actggtcacg tttttttcgt
    22021 tttaataccg cgtggcagtg gggagaacgt gaagaacagg cggtaaaaca attaggcaaa
    22081 cttattcaag aacggctgta atagcgttta atttaattcc tctcaggttg ggtaatatga
    22141 atttcgaata gcagtcatat atcctaactc cttgtctata ctccagaaga taaccttaca
    22201 gagggcataa tgcgcggtag ctcacaacct gattaaattt tctcagggcc gaaggtgtgc
    22261 ctgctagccg ccgtctatgg ttaaacaagg agatattttt acggcacggc ggctgaacaa
    22321 ttaattacga caggagtaag accttatgag caagatattt gcccgcagca gcctgtgtgc
    22381 gctcaccatg acaataatga ccgctcacgc cgccgaaccg cctaccaatt tagataaacc
    22441 ggaagggcga ctggatatta tcgcctggcc gggatacatc gaacgcggac aaactgataa
    22501 acaatacgac tgggtaacgc aattcgaaaa agagacaggc tgcgcggtga atgtgaaaac
    22561 cgccgcgact tccgatgaaa tggtcagtct gatgaccaaa gggggttacg atctggttac
    22621 ggcatccggc gatgcctcgc tgcgtttgat tatgggtaaa cgcgtgcagc caattaatac
    22681 cgccttgatt cccaactgga aagcgctcga tccgcgtgtg gttaaaggcg actggtttaa
    22741 cgttggcggc aaagtttacg gcacacctta ccaatggggg ccgaacctgc tgatgtacaa
    22801 cacgaaaacc ttcccgacgc cgccgaatag ctggcaagtg gtttttgttg agcagaatct
    22861 gccggacggc aagagcaata aaggccgggt acaggcttat gatggcccta tctacattgc
    22921 ggatgccgcg ttgttcgtca aagccacgca gccacagttg ggcatcagcg atccgtatca
    22981 actcaccgaa gaacagtacc aggcggtgct gaaagtgctg cgcgatcaac atagtttgat
    23041 ccatcgctac tggcatgaca ctaccgtgca aatgagcgat ttcaaaaacg agggtgtggt
    23101 tgcttccagt gcatggccct atcaggccaa cgccctgaaa gccgaaggcc agcctgtcgc
    23161 taccgttttc ccgaaggagg gcgttaccgg ttgggctgac accaccatgc tacatagcga
    23221 agcgaaacat ccggtttgcg cctacaaatg gatgaactgg tcattaaccc caaaagtgca
    23281 gggcgatgtg gcggcctggt ttggctcgtt accagtagtg ccgcaagggt gtaaagccag
    23341 tccgttatta ggcgagaaag gttgtgaaac aaacggtttt aactatttcg ataaaatcgc
    23401 cttctggaaa acgcctatag cagaaggggg caagtttgtt ccctacagtc gctggacgca
    23461 ggattacatt gccattatgg gcggtcgcta acttcgctgg ggtgttttat gacgtacgca
    23521 gtggagtttg acaacgtctc acggttgtac ggtgacgtgc gcgcagtaga tggcgtcagt
    23581 attgcgataa aagatggtga gttcttctct atgctggggc cgtccggctc cggcaaaacc
    23641 acttgcctgc gcctgattgc tggattcgaa cagctttccg gcggggctat ctctattttt
    23701 ggtacgcccg ccagcaatct gccaccgtgg gagcgggacg tgaatactgt cttccaggac
    23761 tacgcgctat ttccgcatat gtcgattctt gacaacgtag cctacgggct gatggtcaaa
    23821 ggcgtgaata aaaagcagcg gcatgcaatg gcgcaagagg cgcttgagaa agtggcgttg
    23881 gggtttgtac agcaacgtaa accgtcacaa ctttctggtg gtcagcgcca gcgagtcgcc
    23941 atcgccagag cactggtgaa cgaaccgcgt gtgttgttgc tggatgaacc gctcggcgcg
    24001 ctggatctca aattgcgaga gcaaatgcag cttgagctga aaaagctgca acagtctctc
    24061 ggtatcactt ttatcttcgt tacccacgat cagggcgaag cgttatcgat gtccgatcgg
    24121 gtggcggttt tcaataatgg acgcattgaa caggtcgatt ccccgcgtga tctctatatg
    24181 cgcccgcgca cgccgtttgt tgcgggattc gttgggacat cgaatgtttt tgatggactg
    24241 atggcagaga aactttgtgg catgacggga agcttcgcac tacgaccgga acatatccgc
    24301 ctcaacaccc ctggcgaaat gcaggctaat ggcacgatcc aggcggtgca gtatcagggc
    24361 gcagcgaccc gttttgaact gacactgagc ggcggtgaaa aactgctggt gagtcaggcc
    24421 aatatgacag gcgaagagct gcctgccacg ctcacgcccg gacaacaggt gatggtttcc
    24481 tggtcgcgtg atgtgatggt ggcgctggtt gaggagaggt gaatggcgat gaacgtattg
    24541 caatcacctt cacgtccagg tctgggtaag gtgtccggtt tcttctggcg taatccgggg
    24601 ctggggctgt ttttgctgct gcttggcccg ctaatgtggt ttggcattgt ctatttcggc
    24661 tcgctgctga cgctgttgtg gcagggcttt tatacctttg acgatttcac catgtcggta
    24721 acgccggaac tgacgctggc gaatatccgt gcgctgttta atccggcgaa ttacgacatc
    24781 attctccgca cgctgaccat ggctgtggca gtcactatcg ccagcgccat tctggctttt
    24841 ccaatggcgt ggtatatggc gcgctatacc agcggaaaaa tgaaagcgtt tttttatatc
    24901 gcggtaatgt tgccgatgtg ggcgagctac attgttaaag cctatgcctg gacgttattg
    24961 ctggcaaaag atggcgtggc gcagtggttt ttacaacatc tggggctgga accactgctg
    25021 actgcgttcc tcacgctacc ggcggtgggc ggcaatacgc tgtcaacttc cgggctgggg
    25081 cgctttctgg tgtttctcta tatctggttg ccgttcatga tcctcccggt gcaggcggcg
    25141 cttgagcgtt tgccgccgtc attgttgcag gcgtcggctg atcttggcgc acgtccacga
    25201 caaacctttc gctatgtggt gctgccgctg gcaatcccgg gtattgccgc tggctctatc
    25261 tttaccttct cactcacgct gggcgatttt attgtcccgc agctggttgg ccctccagga
    25321 tattttatcg gcaatatggt ttattcccag cagggggcga ttggcaatat gccgatggcg
    25381 gcggcattca ccctggtgcc gattattctc atcgcacttt acctggcgtt cgtgaaacgt
    25441 ctgggagcgt tcgatgcact ctgaacgcgc accgtttttc ctcaaactgg cggcctgggg
    25501 cggcgtggtt ttcctacatt ttcccatcct gattatcgct gcctatgcgt ttaacactga
    25561 agatgcggcg tttagttttc caccgcaagg cctgacgctg cgctggttta gcgtggcagc
    25621 acagcgtagt gatattcttg atgccgtgac actgtcactt aaagtggcgg cgctggcgac
    25681 attaatcgcg ctggtgttag ggacgctggc agctgccgcg ctgtggcgac gagacttttt
    25741 cggcaaaaac gccatttcgc tgttactgtt gctgcccatt gcgctgccgg gcattgtcac
    25801 tggtctggcg ctattaaccg cttttaaaac catcaatctg gagccgggat ttttcaccat
    25861 cgtggtcggt catgcgactt tttgcgtagt ggtggtgttt aacaatgtca tcgcccgttt
    25921 tcgccgcacc tcgtggagtc tggttgaggc gtcaatggat cttggggcca atggctggca
    25981 aaccttccgc tacgtagtgt tgccgaatct cagttcggcg ttactggctg gaggaatgct
    26041 ggcgtttgcc ttgtcgttcg atgaaatcat cgtgacgact tttacggcag gtcatgaacg
    26101 aacgttaccg ttgtggttgc tcaatcagct tgggcgaccg cgtgatgtcc cggtaaccaa
    26161 cgtggtggca ctgctggtta tgttggtaac aaccttgccg atcctggggg cctggtggct
    26221 aacccgcgaa ggcgacaatg gtcaataacc actgatacag gaatatgcta tgcaacataa
    26281 gttactgatt aacggagaac tggttagcgg cgaaggggaa aaacagcctg tctataatcc
    26341 ggcaacgggg gacgttttac tggagattgc cgaggcatcc gcagagcagg tcgatgctgc
    26401 tgttcgcgcg gcagatgcag catttgccga atgggggcaa accacgccaa aagcgcgtgc
    26461 ggaatgcctg ctgaaactgg ctgatgttat cgaagaaaat ggtcaggttt ttgccgaact
    26521 ggagtcccgt aattgtggca aaccgctgca tagtgcgttc aatgatgaaa ttccggcgat
    26581 tgtcgatgtc tttcgctttt tcgccggtgc ggcgcgctgc ctgaatggtc tggcggcagg
    26641 tgaatatctt gaaggtcata cttcgatgat ccgtcgcgat ccgttggggg tcgtggcttc
    26701 tatcgcaccg tggaattatc cgctgatgat ggctgcgtgg aaactggctc cggcgctggc
    26761 ggcagggaac tgcgtagtgc ttaaaccatc agaaattacc ccgctgaccg cgttgaagtt
    26821 ggcagagctg gcgaaagata tcttcccggc tggcgtgatt aacgtgctgt ttggcagagg
    26881 caaaacggtg ggagatccgc tgaccggaca tcccaaagtg cggatggtgt cgctgacggg
    26941 ttctatcgcc actggggaac acatcatcag ccataccgcg ccgtccatta agcgtactca
    27001 tatggaactt ggtggcaaag cgccagtgat tgtttttgat gatgcggata ttgaagcagt
    27061 ggtcgaaggt gtacgtactt ttggctatta caatgccgga caggattgta ctgcggcttg
    27121 tcggatctac gcgcaaaaag gcatttacga tacgctggtg gaaaaactcg gtgctgcggt
    27181 ggcaacgtta aaatcgggtt cgccggatga cgagtctacg gagcttggac ctttaagctc
    27241 gctggcgcat ctcgaacgcg tcagcaaggc agtagaagag gcgaaagcga cagggcacat
    27301 caaagtgatc actggcggtg aaaagcgcaa gggtaatggc tattactatg cgccgacgct
    27361 gctggctggc gcattacagg acgatgccat cgtgcaaaaa gaggtatttg gtccagtagt
    27421 gagtgttaca ctcttcgaca acgaagaaca ggtggtaaac tgggcgaatg acagccagta
    27481 cggacttgca tcttcggtat ggacgaaaga tgtgggcagg gcgcatcgcg tcagcgcacg
    27541 gctgcaatat ggttgtacct gggtcaatac ccatttcatg ctggtaagtg aaatgccgca
    27601 cggtgggcag aaactttctg gttacggcaa ggatatgtca ctttatgggc tggaggatta
    27661 caccgtcgtc cgccacgtca tggttaaaca ttaaggataa tattgcagat cgtaagagta
    27721 tgagatgatc ttgcgtactg tcagagcact gatttctctg acagtacgtg ctgcgtgcaa
    27781 aatcactaca tcaaataaac cagccaaatc tcatagagat aagagtaaac aaaccaacag
    27841 cagcaaagac atttatcagt actacagttc tactggaaac attcattttt gccacctttt
    27901 atcattgccc ctatgtgtat agtacgggtt aagaaaatcc gcaataatac ttattctgaa
    27961 gatggataag ggcaagttgc tgttgattat tttttctcaa actggtgaaa aaacttgatg
    28021 cacgtcaaaa aatgacgcat atttgcgcgc gttttattca tctggctgga cgcccgtaca
    28081 tgtctctcta tcaacacatg cttgtttttt atgcggttat ggcagcaatc gcatttctta
    28141 tcacctggtt tctttctcac gataagaaac gcatccgttt cttaagcgcc tttctggtgg
    28201 gggcaacatg gccgatgagt ttcccggtgg cgctgttgtt ttcactgttt taaatcttac
    28261 tcactggctt gacggcacca cgcagactta tatcatttgg atgaatcgat aaatttcaca
    28321 agtggctaag gagaaagtat gtcgcatctg gatgaagtca tcgcgcgcgt ggatgccgcg
    28381 attgaagaga gcgtcattgc ccatatgaac gaattattaa tcgccctgag cgatgatgcg
    28441 gagttaagtc gggaagaacg ctatacccag cagcagcgtc tgcgcacagc gatcgcccat
    28501 cacggtcgca aacataaaga agatatggaa gcgcgccacg aacagttaac caaaggtggc
    28561 atcatcctct gattaaaatg tgcgcctggc caccagccag gcaccaacca caagtaaaat
    28621 tgcgccacaa attgggccga tcaacgcccg tagtggtacg ccatggctgc ggaagatatc
    28681 cagcaccaga ccgccaatca gctggctggc gaccagtacc gcaatagtta tcgccgctcc
    28741 cacattctga tatccgctga tactggcgaa gacaaaaaac gatcccagca agccaggtat
    28801 aagtgtccac cagcgcacgt tggacaccag ttcgccaaac cccgccatac cttgtttaaa
    28861 ccataaaata gaaacaaaga gcacaattcc taccaaagag ttcagcaaca tggcgatgag
    28921 aatggtagag gaggtctggg tgatgcgcac catcagcgtg ttctggacta ccagaccaat
    28981 cccggcggca atcaaaaaag caagggtgag cgactgattc atccaatcgc gtccggttca
    29041 gtgcgctcgt cgagttgcaa ctgcataaat gtcagatcca gccaacgacc aaatttagtg
    29101 cctacctgcg gcatttgtgc ggtgacgaca aatcccagcg actggtggag atgcagcgag
    29161 gcctggtttt gcgattcgat cccggcgacc atgacatgct tcccgcaatc ccgcgcttca
    29221 tcaatcaatc ggcttaacaa tttacgcccc agacctttgc cctgatgatc gggatggaca
    29281 taaaccgaat gttccacggt atggcgaaaa ccatcgaaac tacgccagtc gccaaacgag
    29341 gcatatcccg tcactacgcc gttttcctcg ctcaccagca ctggataacc tgctatggtc
    29401 cgcgcttcaa accaggcaat gcggttatct gcatccaccg tttggtcatt ccagatagcc
    29461 gccgtataca acacggcgtg gttataaatt tccgcaatgg cagcacagtc ggctttgcgg
    29521 gcaaaacgga tggacatgtc tggcctcgat aaaataatga tgatgatgtt atcaatacta
    29581 tcgaaaacag cccgaagcaa cggattcctc tatgaaatta ttgcgtgttg cagaggggat
    29641 gtgacggctg caaacaaaac caatcctgtg agcctgctac tattgatgga ttcatgccaa
    29701 aagcacagga gagcattatg gggcaacaaa cgcagcgtaa ccgatgttgg gttctgtcct
    29761 tgcgtccgca tggcgcgccg gttccttacg tctggtttcg gtaaatgtgc tctttgtcgc
    29821 accagcgcaa gcatttacag ttccggaagt gggaaaacgt caattaaatg aaatcaatcg
    29881 gatccgctac gaactggaat taatggcagt tgctctggct gtcgaaaacc tcactatgca
    29941 aaaaaacgtt gctatgttgt gccagcaata cagccgataa ataaaatacg cccggcaaat
    30001 accgggctta aagataatta ctcaaatcgc cacgcaatat ttatgccgac accataattt
    30061 cgtccgggcg caggttcgta atatcgcccg tttgactcat tgacaatgac agaaccaacg
    30121 tattctttat cgaataaatt atcgacacga ccaaataaat ccacggttaa attgtggtaa
    30181 ttgtatttat acccggtgaa taagccgacg agtgtataag aaggcgcttt ggccgtattt
    30241 tcatcatctg ccataatatc gcccatataa cgcgcttccg tgcctgcata ccaaccatct
    30301 tccggtatat aacctatcga cgcaaaggcc atattacggg cgatccctgg catccggtta
    30361 ccgttacaat cctgttcatt gcaaacattg ctgcgatagg tcgcatcaag ccaggtccag
    30421 gacgctttta cgcgaaaatc tcccgcgaaa cgttgatccc atgccagttc agcgccttga
    30481 cgacgggtct ttccggcatt tttgtaagtc gtacgcccac cgctactgct atcgacaaca
    30541 atttcatcat cggtgtcggt ctggaacaat gcgagactaa gcagcccatc accaatacgc
    30601 gttttactgc cgatctcaat tgtatcgttg gtggagggtt ttaacccaaa gttcataccg
    30661 ctttgtccat cagcacgata agacagctca ttaatcgtcg gcgtttcaaa acctcgcccg
    30721 gctgccagat agatattcca ggcatcggtc attgcatatt ttaacgaacc ggcaggtagc
    30781 catttgtgat aactggcatc accgctgtcg tcgccgttac ccggagtaac gtaatggtcg
    30841 ttggaatcaa accacacgga gctgtagcgc acgccagcat ccagcgacag tttttcgctc
    30901 agctgccact gcgtctgtaa atagggatcg acgttccaca tcaaattgcg ttcgtcgcgg
    30961 cgcaactcac ctttttgccc gtattccggc acgccgcgat tcaggcggaa tttattgtaa
    31021 cccttgcggt tttcactcat gttttcgtag ttcaggccgg ttgtgaacgt gactggaacg
    31081 cccaattcac cacggtgtgt ccagcggctg tctatcccct ggtaatggcg ttgcagggta
    31141 atcacgccgc ccgcatgtga cgggttaagt tgcggagcca tcgggattga ctggtactgg
    31201 gtcgtttctc gctctccggc atacatcatt acactcatat catcttgcgc gctcaggcta
    31261 cgttcatagc gcaacccagc ctgagtttgc ttgatggttt ttcgcgtgtc gtactgttct
    31321 gcacggggcg cttgttgtgg attcgccttc cattctgctt cggttagccc acctgggtca
    31381 tctgctttga tatccacact attgaaaatc aggcttaatt tgctggcgtc atcaatgcgt
    31441 acgcccagtt tggcattggc taaatttttc tgtgcgccgc tatggtcacg atagccgtgg
    31501 gtcgtaaaac gtgtggttga gacggtgtaa tcgacatcgc cgggttgtgt gccgtcgccc
    31561 gttgcgcccg tagctttcag cccatagcgc cagctgccaa aactgccgta gtaactactg
    31621 gcttcaatgg ttggtggctg ttgtccggtc tgggtggtga cattcattac accgccagac
    31681 gcgttgccat acagagcaga gaaggggcca cgcagcactt ccacattttg cacactgctt
    31741 aaatcgatgt tagatgtttg cccttgtccg tcgggcatgg tagcgggaat accgtccaca
    31801 tacaggcgaa taccgcgaat tccgtaagtg gagcgggagc caaatccgcg aatcgacagc
    31861 tgtaaatctt gcgcatagtt ctgccggttt tgtacctgta aaccaggcac tccggtcagt
    31921 gattcagaca agttaatgcg cggtgttgcc aggcgcatct cctcgccatc caccacgctc
    31981 acggctgctg gggtatccag ttctgaaacc acctgcggtg cggcactgac aatcatggtc
    32041 tgttcatcag cggcaaaaac aacgggggaa aggacaagca gtgcgggcaa aacggtctgt
    32101 cggacggaaa aaatcttcat gaaaaaagcc aggttaagaa tgggaaaaca ccgtcatggt
    32161 aatgaaattg taaatttatg gaaaacgaaa cggcacaata tgttaagtaa ttgagaaaat
    32221 tgtagtcgta acggtaagaa atgctccaca tttgagaaaa taatgattac cattcccatt
    32281 tataacaagg gcgtaacgat gattacgctt agcgaagcat tgtgaagcag caaaaatatc
    32341 ggttcatcaa agggagtcgt catgcattta cgtcatctgt tttcatcgcg cctgcgtggt
    32401 tcattactgt taggttcatt gcttgttgct tcatcattca gtacgcaggc cgcagaagaa
    32461 atgctgcgta aagcggtagg taaaggtgcc tacgaaatgg cttatagcca gcaagaaaac
    32521 gcgctgtggc tcgccacttc gcaaagccgc aaactggata aaggcggcgt ggtttatcgt
    32581 cttgatccgg ttactctgga agtgacgcag gcgatccata acgatctcaa gccgtttggt
    32641 gccaccatca ataacacgac tcagacgttg tggtttggta acaccgtaaa tagtgcggtc
    32701 acggcgatag atgccaaaac gggcgaagtg aaaggtcgtc tggtgctgga tgatcgtaag
    32761 cgcacggaag aggtacgccc gttgcaacca cgtgagctgg tagctgatga tgccacgaac
    32821 accgtttaca tcagtggtat tggtaaagag agcgtgattt gggtcgttga tggcgagaat
    32881 atcaaactga aaaccgccat ccagaacacc ggtaaaatga gtaccggtct ggcgctggat
    32941 agcaaaggca aacgtcttta caccactaac gctgacggcg aattgattac catcgacacc
    33001 gccgacaata aaatcctcag ccgtaaaaag ctgctggatg acggcaaaga gcacttcttt
    33061 atcaacatca gccttgatac caccaggcag cgtgcattta tcaccgattc taaagcggca
    33121 gaagtgttag tggtcgatac ccgtaatggc aatattctgg cgaaggttgc ggcaccggaa
    33181 tcactggctg tgctgtttaa cccagcgcgt aatgaagcct acgtgacgca tcgtcaggca
    33241 ggtaaagtca gtgtgattga cgcgaaaagc tataaagtgg tgaaaacgtt cgatacgccg
    33301 actcatccga acagcctggc gctgtctgcc gatggcaaaa cgctgtatgt cagtgtgaaa
    33361 caaaaatcca ctaaacagca ggaagctacc cagccggacg atgtgattcg tattgcgctg
    33421 taatagatat ggcgagggtg aaaacggaaa gaattgcctg acttttatca cgccgcatcc
    33481 ggcaccttcg tcagtagtct gaatttgccc ggcacgtagc cgggcaattt tgcaattaat
    33541 tcgacgtttc aggcttaaac acaatttcct gtttttcttc atcttcctcg acgactggcg
    33601 cagtgctgtg aatttcagca acgcgtttgc gcacaccaaa ccagcctata accagcagaa
    33661 taccgataat cggcagtgcc gcgatggtat aagtcccgtt cgggtaatcg aacgccatca
    33721 gcaccaggac actcagtaaa aacagtaatg tcagccagga agtgaagggc gcgccaggca
    33781 gtttaaaact gacatccgct gctttgcctt ctttaattgc tttacgcagg cgcatctggc
    33841 aaacgatgat aaacgcccat gaagcgatga ttcccagcga cgcgaagttc aacacaatct
    33901 caaatacgcg tgacggcacc agatagttga ggaatacgcc gacgacatac acaaccagtg
    33961 tcgccagaat cccggcatac ggcacatgct gacgactcat tttcgccata aaactcggtg
    34021 cggaaccgcc catcgccatc gagcgcagaa tacgcccggt gcagtacaga cctgaattca
    34081 ggctggagag ggcagcggtc agcaccacaa tgttcataat gctgccgatg tacggcacac
    34141 ccagtttaga gaaaaacgtc acgaacggac tttgccccgc ctgatacgcg ctccacggca
    34201 ataacataac cagcaacacc acggagccga cgtaaaacag gccaatacgc caaatcacac
    34261 tgttaatcgc tttaggcacc atggtctgcg gatctttaca ttctccagcc gctgtaccca
    34321 ccatttcaat ggaggcaaaa gcaaacacta cgccctgaat caacaccagc gcaggcagca
    34381 aaccgtgggg gaagaagccg ccattatcgg tgattaaatg aaagccagtg gtgttgccat
    34441 ccagcggctg accactaccg aggaacactg tacccacgac caaaaaggtt acaatggcga
    34501 gcactttaat aagcgcaaac cagaactcca tctctgcaaa ccatttcaca ccgatcatat
    34561 tcatggtgcc aacgatggtc agtgcagcga gcgcaaagac ccactgcggc acgccgccaa
    34621 acgcgcccca gtaatgcata tacagagcga cggcggtaat atcaacaatc cccgtcatcg
    34681 cccagttgat gaagtacatc cagccagcaa cataagcggc cttctcaccc aaaaactcac
    34741 gggcataaga gacaaaactg ccactggaag ggcggtgtaa caccagctca cccaatgcac
    34801 gcagaataaa aaacgaaaac aggccgcaga tcaaatagac cagtgccagt gcgggccccg
    34861 ccatttgcag tcgcgctccc gcgcctaaaa acaaacccgt accaattgcg ccgccaatgg
    34921 cgatcatctg tacctggcga ttgcccatcg ctttgtgata cccctcttcg tgggcattaa
    34981 gccagcggcg tttcgcggcg tgttgatctg aagtgtcggt gttgtgttta ctcattgctc
    35041 tccctgattg ctttaatgaa aaagtcatat aagttgccat gaacaatgtt tattcatcct
    35101 ggcgatgaca agattcacca ttattcttta tgggtataac tgtcggcgca gaatcctacc
    35161 ttttatcatg taacgatgca aaataatccc gctataaata tgtggggata aaggcggctg
    35221 gcgacaaaat agcaacctga tgaaattatg acggaaacaa ccactattgg tttgatgata
    35281 cctgactgca gtcctactct tgcgccactc gtgcgtcgct ctgaacgcca gtggttgcag
    35341 tggacactgg cgtggttggc tcaatgaata gtgaggggct agcacatgat gggtttcagg
    35401 ggtaatacgc caaataccaa tacaattgct gattaaatga gccaattgcc gacgttacag
    35461 aaaaggctaa aaaatgtaag aggcatgtag ggtgataaac gcgctattgc ccaacaggag
    35521 tttctggaat tagtcctgtt ccatgggcat tacaactctt aggacattac ttcaatataa
    35581 ttaatgtgta tatcat
//
LOCUS       DAZFJL010000032        34998 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFJL010000032 DAZFJL010000000
VERSION     DAZFJL010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.032"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(61..792)
                     /gene="rlmB"
                     /locus_tag="ACHJ8C_001591"
     CDS             complement(61..792)
                     /gene="rlmB"
                     /locus_tag="ACHJ8C_001591"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO5551465.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(883..3324)
                     /gene="rnr"
                     /locus_tag="ACHJ8C_001592"
     CDS             complement(883..3324)
                     /gene="rnr"
                     /locus_tag="ACHJ8C_001592"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO5551466.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3363..3788)
                     /gene="nsrR"
                     /locus_tag="ACHJ8C_001593"
     CDS             complement(3363..3788)
                     /gene="nsrR"
                     /locus_tag="ACHJ8C_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO5551467.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3993..5291)
                     /gene="purA"
                     /locus_tag="ACHJ8C_001594"
     CDS             complement(3993..5291)
                     /gene="purA"
                     /locus_tag="ACHJ8C_001594"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO5551468.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5395..5592)
                     /gene="yjeT"
                     /locus_tag="ACHJ8C_001595"
     CDS             complement(5395..5592)
                     /gene="yjeT"
                     /locus_tag="ACHJ8C_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO5551469.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5674..6678)
                     /gene="hflC"
                     /locus_tag="ACHJ8C_001596"
     CDS             complement(5674..6678)
                     /gene="hflC"
                     /locus_tag="ACHJ8C_001596"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO5551470.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6681..7940)
                     /gene="hflK"
                     /locus_tag="ACHJ8C_001597"
     CDS             complement(6681..7940)
                     /gene="hflK"
                     /locus_tag="ACHJ8C_001597"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO5551471.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSTTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8026..9306)
                     /gene="hflX"
                     /locus_tag="ACHJ8C_001598"
     CDS             complement(8026..9306)
                     /gene="hflX"
                     /locus_tag="ACHJ8C_001598"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO5551472.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLDDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9383..9691)
                     /gene="hfq"
                     /locus_tag="ACHJ8C_001599"
     CDS             complement(9383..9691)
                     /gene="hfq"
                     /locus_tag="ACHJ8C_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO5551473.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9777..10727)
                     /gene="miaA"
                     /locus_tag="ACHJ8C_001600"
     CDS             complement(9777..10727)
                     /gene="miaA"
                     /locus_tag="ACHJ8C_001600"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO5551474.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10720..12567)
                     /gene="mutL"
                     /locus_tag="ACHJ8C_001601"
     CDS             complement(10720..12567)
                     /gene="mutL"
                     /locus_tag="ACHJ8C_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO5551475.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     LDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12577..13908)
                     /gene="amiB"
                     /locus_tag="ACHJ8C_001602"
     CDS             complement(12577..13908)
                     /gene="amiB"
                     /locus_tag="ACHJ8C_001602"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO5551476.1"
                     /translation="MMYRIRNWLVATLLLLCAPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSYYTVVFTINADAPPSPPPMVAKRVETPAVVAPRVSEPARNPFK
                     TESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTI
                     AIARKLRTLLNNDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSA
                     TGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQFGH
                     SQRVGYDVATSMINQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERL
                     LASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13927..14388)
                     /gene="tsaE"
                     /locus_tag="ACHJ8C_001603"
     CDS             complement(13927..14388)
                     /gene="tsaE"
                     /locus_tag="ACHJ8C_001603"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO5551477.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLLVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14360..15907)
                     /gene="nnr"
                     /locus_tag="ACHJ8C_001604"
     CDS             complement(14360..15907)
                     /gene="nnr"
                     /locus_tag="ACHJ8C_001604"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO5551478.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAIGIDVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANTH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSQWLIPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCTQRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKMSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15906..17045
                     /gene="queG"
                     /locus_tag="ACHJ8C_001605"
     CDS             15906..17045
                     /gene="queG"
                     /locus_tag="ACHJ8C_001605"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO5551479.1"
                     /translation="MSEPLDLNQLAQNIKQWGLELGFQQVGITDTDLSASEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17028..17081)
                     /locus_tag="ACHJ8C_001606"
     CDS             complement(17028..17081)
                     /locus_tag="ACHJ8C_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5551480.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17315..17390)
                     /locus_tag="ACHJ8C_001607"
     tRNA            complement(17315..17390)
                     /locus_tag="ACHJ8C_001607"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17355..17357),aa:Gly,seq:gcc)
     gene            complement(17426..17501)
                     /locus_tag="ACHJ8C_001608"
     tRNA            complement(17426..17501)
                     /locus_tag="ACHJ8C_001608"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17466..17468),aa:Gly,seq:gcc)
     gene            complement(17659..17734)
                     /locus_tag="ACHJ8C_001609"
     tRNA            complement(17659..17734)
                     /locus_tag="ACHJ8C_001609"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17699..17701),aa:Gly,seq:gcc)
     gene            complement(17945..18490)
                     /gene="orn"
                     /locus_tag="ACHJ8C_001610"
     CDS             complement(17945..18490)
                     /gene="orn"
                     /locus_tag="ACHJ8C_001610"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO5551481.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18585..19637
                     /gene="rsgA"
                     /locus_tag="ACHJ8C_001611"
     CDS             18585..19637
                     /gene="rsgA"
                     /locus_tag="ACHJ8C_001611"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO5551482.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19734..20702
                     /gene="asd"
                     /locus_tag="ACHJ8C_001612"
     CDS             19734..20702
                     /gene="asd"
                     /locus_tag="ACHJ8C_001612"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO5551483.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVDLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20724..24047
                     /gene="mscM"
                     /locus_tag="ACHJ8C_001613"
     CDS             20724..24047
                     /gene="mscM"
                     /locus_tag="ACHJ8C_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO5551484.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLDRIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     NDALNQEILQISSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24198..25700)
                     /gene="yjeM"
                     /locus_tag="ACHJ8C_001614"
     CDS             complement(24198..25700)
                     /gene="yjeM"
                     /locus_tag="ACHJ8C_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO5551485.1"
                     /translation="MPHSIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWHIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHFSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNTMGMPSIAMWMQCGLVTIFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(25919..26896)
                     /gene="epmA"
                     /locus_tag="ACHJ8C_001615"
     CDS             complement(25919..26896)
                     /gene="epmA"
                     /locus_tag="ACHJ8C_001615"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO5551486.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            27221..29029
                     /gene="frdA"
                     /locus_tag="ACHJ8C_001616"
     CDS             27221..29029
                     /gene="frdA"
                     /locus_tag="ACHJ8C_001616"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO5551487.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            29022..29756
                     /gene="frdB"
                     /locus_tag="ACHJ8C_001617"
     CDS             29022..29756
                     /gene="frdB"
                     /locus_tag="ACHJ8C_001617"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO5551488.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            29767..30162
                     /gene="frdC"
                     /locus_tag="ACHJ8C_001618"
     CDS             29767..30162
                     /gene="frdC"
                     /locus_tag="ACHJ8C_001618"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO5551489.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIMIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            30173..30532
                     /gene="frdD"
                     /locus_tag="ACHJ8C_001619"
     CDS             30173..30532
                     /gene="frdD"
                     /locus_tag="ACHJ8C_001619"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO5551490.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            30595..31728
                     /gene="ampC"
                     /locus_tag="ACHJ8C_001620"
     CDS             30595..31728
                     /gene="ampC"
                     /locus_tag="ACHJ8C_001620"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO5551491.1"
                     /translation="MLKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            31817..32350
                     /gene="blc"
                     /locus_tag="ACHJ8C_001621"
     CDS             31817..32350
                     /gene="blc"
                     /locus_tag="ACHJ8C_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO5551492.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(32347..32664)
                     /gene="sugE"
                     /locus_tag="ACHJ8C_001622"
     CDS             complement(32347..32664)
                     /gene="sugE"
                     /locus_tag="ACHJ8C_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO5551493.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSLA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(32840..32986)
                     /gene="ecnB"
                     /locus_tag="ACHJ8C_001623"
     CDS             complement(32840..32986)
                     /gene="ecnB"
                     /locus_tag="ACHJ8C_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO5551494.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(33097..33222)
                     /gene="ecnA"
                     /locus_tag="ACHJ8C_001624"
     CDS             complement(33097..33222)
                     /gene="ecnA"
                     /locus_tag="ACHJ8C_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO5551495.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(33274..33840)
                     /gene="efp"
                     /locus_tag="ACHJ8C_001625"
     CDS             complement(33274..33840)
                     /gene="efp"
                     /locus_tag="ACHJ8C_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO5551496.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            33882..34910
                     /gene="epmB"
                     /locus_tag="ACHJ8C_001626"
     CDS             33882..34910
                     /gene="epmB"
                     /locus_tag="ACHJ8C_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO5551497.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         8330 a         9591 c         8886 g         8191 t
ORIGIN      
        1 accagctata gacaaaaaaa aaccatccaa atctggatgg cttttcataa ttcagaggaa
       61 ctagctgcgc tgacgaaccg cttcaaataa gcaaattccg gttgcaaccg aaacgttcag
      121 ggaagaaaca cttcctgcca ttgggatgct gatcaactca tcgcaatgtt cacgggtcag
      181 gcgacgcata ccttcacctt ccgcgcccat caccagcgcc aggcgtccgg tcattttgct
      241 ctgatagagt gtatgatccg cctcgcctgc cgtaccgacg atccagatat tctcttcctg
      301 caacatacgc atggtgcgcg ccagattagt cacccgaatc agcggaacgc tttctgccgc
      361 accacaggcc acttttttcg ccgtggcgtt gagctgtgcg gagcgatctt tcggcacaat
      421 caccgcatga acaccagcgg cgtccgcgct acgcagacac gcgccgaggt tgtgcggatc
      481 ggttacgccg tcgaggatca gcaggaacgg ttgatcgagc gaagcgatca gatccggcag
      541 atcgttttcc tgatactgcc gtcctggctt cacacgagcg ataatgccct gatgcacggc
      601 accgtcgctt ttctcatcga gatattggcg gtttgccaac tggataacca cgccctgaga
      661 ctcaagggcg tgaatcagcg gtaacagacg tttatcttca cggcctttta aaataaagac
      721 ttcctgaaaa cgttcagggg cgcgctccag cagggcctgc actgcatgga tgccgtaaat
      781 catttcgctc attaatgtac tcgttgttta cgttttgccg ggagcgccac gcttgcccgg
      841 cctacggata attcaccctc ttctgtttga agagggtaat gatcactctg ccactttttt
      901 cttcgccgca cgcttcgctt tggtcgctgc ggctattttc tgcgttttcg ccgatggctt
      961 tttcgctttt ctcgcgtctt tcttcgctgc tttcggcttc ggttttttct cgccgcggaa
     1021 ggcgctgtct ggctcaaagt tgaccttttt gccgacctga cgccgcttgc cgcctttttt
     1081 gcctgcatcg ccttttttcg ctttctcgcg cgccgtttta ccgacgttgc gcggtgcgcg
     1141 ttcgctggag atcaggctaa agtcgatttt gcgctcgtcc atattaaccg cttcgacgcg
     1201 aacttccacg cgatcgccca ggcgataagt ctggccgctg gattcaccca tcaggcgttg
     1261 ccctacctgg tcaaagcgat agtagtcatt gtccagcgaa gagacatgga ccagaccatc
     1321 aatgaacaag tcgtccagac ggacgaagaa gccaaagcca gtgacgctgg aaattacgcc
     1381 tttaaagacg ttacctacct ggtcgagcat gaagtcacac ttcagccagt cagacacatc
     1441 gcgcgttgct tcatcggcac gacgttccgc catcgaacag tgctgaccca gttgcagcat
     1501 ctcttccatc gaataatggt agccgccggt ttcagtggtg ttgccctgat gcccctgctc
     1561 tttcgccagc agatatttaa tggcgcggtg caacgtcagg tcaggataac gacgaatcgg
     1621 cgaagtaaag tgcgcatagg actgcaatgc cagaccgaag tgaccacggt tttccggatc
     1681 gtaaatcgcc tgtttcatcg agcgcaaaag catggtttgc agcatttctg catcaggacg
     1741 atcggcaacc gattccagta actctgcata gtcgcgcggt tccggcttgt taccgcccgg
     1801 caattccagc cccagctccg ccagcactga acggaaagag gtaatcgctt cggtgctcgg
     1861 cttgtcgtga atacggaaca gtgccggttc tttggctttc tcaacgaaac gcgccgccga
     1921 gatattcgcc agaatcatgc actcttcaat taacttgtgg gcgtcgttac gctgagtctg
     1981 ttcgatacgt tcaatacggc gttcagcgtt gaaaatgaac ttcgcttctt cgctctcaaa
     2041 tgagatccca ccgcgttctt cacgggcttt atccagcact ttatagaggt tatgtaactc
     2101 ttcgagatgc ttaaccagcg gcgcatactg ctcgcgcaga tcctgatcgc cttgcagtat
     2161 atgccagact ttggtgtagg tcagacgcgc gtgagaactc atcaccgctt cgtagaattt
     2221 gtagccagtc aggcggcctt tcgacgaaac cgtcatctcg cacaccatac acaggcggtc
     2281 tacctgcggg ttgagcgagc acaggccgtt agagagcact tccggcagca tcgggataac
     2341 ctgcgaaggg aagtacaccg acgtaccacg attacgcgct tctctgtcca gcggcgttgg
     2401 cggacgcaca tagtagctga cgtcggcaat cgcgacccat aaacgccagc cgccgccgcg
     2461 ttttttctca cagtaaactg catcgtcaaa gtcacgggcg tcttcgccat caatggtgac
     2521 cagcggtaaa tcgcgcagat cgacacggcc cgcttttgct tcttccggca cttcttcttt
     2581 cagcccggca acctgttgct caacagcctg cggccagatg tacggaattt catgggtacg
     2641 cagagcaata tcaaccgcca tgccggtgcc catattatcg cccagcactt cgacgatttt
     2701 acccaccgct ttggtacggc gagtcgggcg ttgagtcagt tcaaccacca ccacaaagcc
     2761 catccgcgcg cccatgatct gatcgggcgg gattaagata tcgaagctca gacggctatc
     2821 gtcaggaacc acaaagccga cgcccgcttc ggtgaagtag cgaccaacaa tctggctggt
     2881 ttttggcacc agtacgcgga caatacgcgc ttcacgacga cctttacggt cagcgcccag
     2941 cggctgagcc agcacctgat cgccatgaat gcaggttttc atctgctcgc tggagagata
     3001 caaatcatct ttacgccctt caactcgcag aaagccgtag ccatcacggt ggccaataac
     3061 ggtacctttc accaggtcga ggcgttccgg cagcgcatag cactgacggc gagtgaagac
     3121 cagttgacca tcgcgctcca tcgcgcgcag gcggcgacgc aggccttcaa gctgctcttc
     3181 gccttcaatg tgcagttcta ccgccagctc atcacggctg gccggttttt cacgtttggt
     3241 taaatgttcg aggataaatt cccgactagg gatgggattc gcgtattttt cagcttcgcg
     3301 ttcctggaaa ggatcttgtg acatctcggt tcctccgttg tcatctctga tgaagatttt
     3361 cgtcactcca ccagcaataa tttataaagc ggttgattct cttcaaccaa atcggcaagc
     3421 gtgtagttat ccagttccgt aagaaaactt tgcacggcct tagaaagcgc ctgtttcaac
     3481 cgacaggcag gtgtaatgtg gcaaaactca ctgctgcaat tcaccagcga taagggctcc
     3541 agctcgcgca ccacatcacc aatacgtatt gcactcgccg gtttacccaa gcgaatgccg
     3601 ccattttttc cacgaacagc agtcacgtag ccggcacgac taagttgatt gattattttg
     3661 accatatgat tacgggagac gccgtagacg tcagtcactt cagaaatact ggtcatccgc
     3721 ccttctggca atgacgccat gtagatcagc gcacgtaatc cgtaatcagt gaaactcgtt
     3781 aactgcacat caacctcaaa agggaaatcg gggaaaaata catttatatt gatgataaac
     3841 cagccacaag ctgtgtcgct aatttatttc agttcgggga aggaaaaaag cgaggattta
     3901 acactgtgtc ggatagcgta atggcttaac cgacacagca atgtatcaca ctgttagccc
     3961 ggcaggcaaa atatctgcca ggcgtaccag aattacgcgt cgaacgggtc gcgcagaatc
     4021 atggtttcag tacgatccgg accggtagag atgatatcga tcggcacacc ggtcagctct
     4081 tcaatgcgct tgatgtagtt cagtgccgcc tgcggcaggc cactacgatc tttcacgccg
     4141 aaggtggatt cagaccagcc cggcatggtt tcgtaaatcg gctctacacc tttccagtcg
     4201 tcagctgcca gcggagtggt agtcacttca cgaccatccg gcatacggta agccacgcag
     4261 agtttaacct ctttcaggcc atccagaacg tccagtttag tcaggcagaa gccagacagg
     4321 gagttcagct gtaccgcacg acgaacggca acggtgtcca gccagccggt acgacgacga
     4381 cgacccgtag ttgcgccgaa ttcgttaccc tgcttgcaga ggaactcgcc cgtttcatca
     4441 aacagttcgg tcgggaacgg acctgcaccc acacgagtgg agtaagcttt gaggataccc
     4501 aaaacgtaat caacataacg cgggcccagg ccggaaccgg tcgccacgcc accagcagta
     4561 gtgttggaag aagttacgta cggataagtg ccgtggtcga tatccagcag cgtaccctgc
     4621 gcaccttcaa acatgacgaa atcgccacgc tgacgcgcct ggtcgagcag atcagaaacg
     4681 tcaacaacca tagaagtcag gatgtcggca acagccatcg tatcatccag aactttctgg
     4741 taatcaaccg cttcagcttt gtagtagtta accaactgga agttgtgata ttccatcact
     4801 tctttcagtt tttcagcgaa ggtttctttg tcgaaaaggt cgccaacacg cagaccgcga
     4861 cgtgccactt tatcttcata agcaggcccg ataccacgac cggtggtgcc gatcgctttc
     4921 gcgccacgcg ctttctcacg cgcgttatcc agcgcaacgt gataatcaag gatcagcgga
     4981 catgcttcgg acagcagcag acgctcacga acggggatgc cacggtcttc cagttctttc
     5041 atctctttca tcagcgcagc cggagacagc acaacaccgt taccgatgat gctggttaca
     5101 ttctcgcgga gaatacctga tggaataaga tggagaacgg ttttttcacc gttgattacg
     5161 agagtatggc ctgcgttgtg accgccctgg tagcgtacaa catatttagc ccgttcagtc
     5221 agaagatcga cgatcttacc tttaccttcg tcaccccatt gggtgcccag tacgacgacg
     5281 ttgttaccca tttttcaaaa tcaccgtttg cttaaaaatg gattctacca tcgctttttc
     5341 agagttacag cactttttgc actcaaaaat gacctcgatc agtctgtttt ttgttcagcc
     5401 aatcgttttc ctcaacatgt agtagaccac aacgcccgca accacaagtc caccgccaaa
     5461 acgacgtaaa atattatcgg gcaaattggt catcgcagag atcatcttct tccatgcctt
     5521 cgggtaaagc atcggcccta aaccttccag taccaaaacc agggcaagcg ccagccagat
     5581 tgtcgaattc attcattatc cttatagaaa aagaaaacca ccgacatccc tgaggatgcg
     5641 gtggctttat tgacctgtac cgcagtcgtt atattaacgc gttgcggagg tcggcgtctt
     5701 catgtagcgg aagaaatcgc tgtccgggct catgaccatc acgtcctgat tgccagagaa
     5761 gctgttctca taagcacgca ggctacggat gaatgcgtag aagtccggat ctttactgaa
     5821 tgcatcagca aacagtttgg ctgcttcggc atcgccttca ccgcgcatga tgcggccctg
     5881 acgctcagct tctgccagcg ttctggtcac ttcatagtcg gcagttgcac gcagtttttc
     5941 cgcttcttcc tgaccttgtg aacggtgacg acgcgctacc gcttcacgct cggcgcgcat
     6001 acggttgtag atcgcttcag acacttcggt cggcaggttg atctgcttaa ttcgcacatc
     6061 gacaacttca atacccagcg ccgccatact gttcgggttg atgaccggaa ctttgccctt
     6121 cgtctcagcc gttacgcgct ctgccgcttc ggcaatggcg ttatctgccg ccggggtagt
     6181 aacttcatct tctgtacccg cagaaccgga gttcagcgcg tcacgtactt cgagggtcag
     6241 acgaccacgg gaatcggtga cgatgtcttt cacgtccaga cgaccaattt cagaacgcag
     6301 acggtcagag aacttacgtt tcaacagcac ttccgcttgc gaaatgtcgc caccgcccgt
     6361 tgccaggtag taacggctga aatcgctgat acgccatttg atgtaagagt cgacgatcag
     6421 gtctttcttc tctttggtca caaagcggtc ggcctggttg tccatggtct gaatacgtgc
     6481 gtcgagcatt ttcaccgttt caatgaacgg tatcttgaaa tgcagacccg gctcataaac
     6541 cagaggtttg ttgtcatcgt cacgcagtac cttaccaaaa cgcagcgtaa tgccacgctc
     6601 accttctttg acgacaaaga cagacatgta aagcactacc agcacgatga tgataatcgc
     6661 gataactgac ttacgcatcg ttattccccc tgacgctggt agtcgttacg ctgcgcgttg
     6721 gcgcggcgtt ggtccataat atcgccctga ctggtggacg acgtgttgct tgctccactg
     6781 gttgtggtgg aagaggctgg cggcagacgc agcagattgc tggcaccgtt atcgctcttc
     6841 gccgcagggg cgttaccacc tttcagcatc tggtctaacg gcagaaccat caggttgcca
     6901 cctttatcgt taaccagcac tttgcgggtg ttgcccaaca ctttttccat cgtctcgata
     6961 tacagacgct cgcgagtaat ttccggcgcg gctttatatt ccggcagaag tttagcaaag
     7021 cgcgccactt caccctgagc ttccaggatg gtctgggcct tgtacgcacg cgcctcttcg
     7081 aggatacgtt gcgcctgacc gttcgcacgc ggctgaactt cgttggtata cgcttctgct
     7141 tcacgaatgt attgctgttc gttttcacgc gcggcaatcg catcgtcaaa cgccgctttt
     7201 acttcttccg gcggacgagc agcctggaag ttgacgtcca gcagcgtgat acccatgtca
     7261 tacggacgaa tcgtctcttc cagttcgcgc tgagtatcgc tacgaatcac ggtacgacct
     7321 tccgtcagaa tgcggtccat ggtgtattta ccgataaccc cgcgcagggc gctgtcggta
     7381 gcctgacgca gactgtcatc cgggctggtc acgctataca gatatttttc cggatcggtg
     7441 acgcggtact gcacgttcat ctcaacgcgc actacgttct cgtccgacgt cagcatcaca
     7501 ccagaagctg ccagttcacg cacggcttcc acgttcaccg gtttgacttc gtcgataaac
     7561 gtcggtttcc agttcagacc cggctcaacc agatggctga atttaccaaa gcgtgttacc
     7621 acgccgcgtt cggcttcttt aatggtatag aaaccactgg ccgcccagat aatgacaatc
     7681 gctgccgctg cgatggtaac gacgcgaccg ccaagctgcg ggcgcgggcc ttgcgatgaa
     7741 ctgccaccgc cagatccggt gcctttaccg ccgcccagac caccgagctt tttgctcagt
     7801 ttgcggaaga tatcatctaa atcaggtggc ccttgatcgc gaccgccttt gtttccattt
     7861 ccctcagagt tgccgccagg tttgctgctt ccccacgggt cgcggtcttg tccgttatta
     7921 ccgggctgat tccacgccat gtttgtgctc catatttgtt atgcggtgat ccctgttgtc
     7981 tttcgacgcc agaggatatg actccacgct tcagacgcta cgccgttaga tcaggtaatc
     8041 gatcaacgcc ggttcttgtt tgcagaggcg acgccagtca acgatcggca tacgaacttg
     8101 cagacttacg ctgccgtcct cctccatcca ctctttttct attgcctgaa gctgataaaa
     8161 acgacttctc agacgccctt cctgcggcgg cagacgcaat gtatgctgcg ccacctcgcc
     8221 ggaaagccgc tccgtcaaag cctgaaaaag ctgtggtatc cccgctccgg tctgcgcaga
     8281 aagccagaca cggatcggtt tgttctcttc atctctgtca atacgcggtt cgaaatcgtc
     8341 cagcatatcg atcttgttca tcaccagcag ggttgggatc tcgtgagcat caatctcttc
     8401 aagaaccgta ttcaccgctt cgatgttttc ttgtacacgc acatccgccg cgtcaatgac
     8461 gtgcagcagc aatgtggctt gccgcgtctc ttgtaacgtg gctttaaatg ccgccaccag
     8521 atcgtgcggc aggtggcgaa taaaccctac ggtatctgcc aacacggttt caccgacatc
     8581 cgcaacgtca atacgccgca acgtcgggtc gagggtggca aacaactggt ctgccgcgta
     8641 gacccgcgct tcggtgatgc gattgaaaag agtagattta ccggcgttgg tatatcccac
     8701 cagcgaaaca gtaggaacgt cggctttgat acgcgattgc cgcccctgct cacgctgctt
     8761 ttcaactctt tccaggcgcg actgtatctg cacgatgcga ttacgcaaca aacgacggtc
     8821 ggtttcgagc tgggtttcac ccggaccacg caaaccaatc ccgcctttct gtctttcaag
     8881 gtgggtccag ccacgcacca ggcgcgtagc cagatggcgc aactgcgcca gctcaacctg
     8941 caacttaccc tcatgggtac gcgcacgttg ggcgaaaata tctaaaataa ggccggtgcg
     9001 gtcgataaca cgacactcgc acaaacgctc caggttacgc tcttgcgccg ggctcagggc
     9061 atggtcaaaa agaacgaccg aagcacccgt cgctttgaca gcttccgcaa tttcaactgc
     9121 tttaccttca cctacaaaat actttgggtg cggcgcttta cggctaccgg taatcacctg
     9181 caatgcttcg acaccggcgg aagagaccag agattcaaac tcctggagat cttccatatc
     9241 tttgtcttgc gtaaaataga tgtgtaccag taccgcctgc tcaccagcat cataacggtc
     9301 aaacaagcgt ataaccctct aaatagatca gcggggaacg caggatcgct ggctccccgt
     9361 gtaaaaaaaa cagcccgaaa ccttattcgg tttcttcgct gtcctgttgc gcggaagtat
     9421 tctgcgcgct gctaccatga tggtaattac tgctggtacc gccaccggcg ttgttactgt
     9481 gatgagaaac cgggcgagac gggacaacag tagaaatcgc gtgcttgtaa accatctggc
     9541 tgaccgtgtt tttcaacagg atcacgaact gatcaaaaga ctcgatttgc ccttgcagct
     9601 taataccatt caccaaataa atagaaactg gaacacgttc ccgacgcagt gcgttcagga
     9661 acggatcttg taaagattgc cccttagcca ttctctcttt tccttatatg cttatttgta
     9721 ctttgaacct ttcgattctg aaaaaattgc gcacgatacg tctcaattgt acacattcag
     9781 cctgcgatag caccaacaac ctgtaatact tcgtcacgcg cttgttctgg tttttcactg
     9841 tcaagccagt gaaccccttc ccaaccacgc agccaggtta tctgccgctt cgccaactgt
     9901 ctcgtggcgc aaacacctcg ataaaccatt tcatcgtatg agatttcgcc ttcaaggtaa
     9961 gaccacatct ggcgatatcc cacgcaacga atggaaggca aatccgtatg caaatctcct
    10021 cgggcaaaaa gcgcccggac ttctgcttca aaacctgaag ccaacatctg atgaaaacgc
    10081 tgctcaattc gttgatggag cagttcacgg ctcgccgggg cgatggcgaa ctgatgcacc
    10141 tgatacggta gagcgtctcc tgacgtttgc gtcagttccg ttaaagtttt acccgaaatg
    10201 aaaaaaactt ccagtgcccg ggaaagcctt tgtggatcat ttggatgaat ccttgctgcc
    10261 gcaaccggat ctacctcctg aagttgacga tgcaatgact cccaaccttg ctctgccgcc
    10321 tgttgctcaa ttctggcccg cacttccggg tctgccgacg gtagcggcga caacccttcc
    10381 agcaatgcct tgaaatacaa catcgtaccg cccactaaca gtgggatccg ccccgccgcg
    10441 gtgatatcgg ccatttccgc cagcgcatcg cggcgaaaat cagcagccga gtaagcctgc
    10501 gacggatcgc gaatatccag caatcggtgc ggcgcggcga gtaactcttc agcgttcggc
    10561 ttcgccgtcc cgatatccat ccctttgtaa ataagggcag aatcaacgct tatcaactct
    10621 actggtaaaa ttttacgcag ctcaatggct aacgccgttt taccggaggc cgtcggcccc
    10681 atcaaaaaaa tcgccttagg caggctcgcc ttactgatat cactcatctt tcagggcttt
    10741 tatcgccgga tgtaaatcaa cagattgtaa cagaccaccc ggcggcgttt tcacaagttg
    10801 cggacataac cgttccacgt ccgccagcag ggttatggcc tgcgccattg accactgcgc
    10861 atgttcgctc atcagatttc gtgcaatcca ctgcgcaata ttgccaggtt cgaatacgga
    10921 ctgcttcgcc aggtagccta tcagttcagg aatcaagatt tgtaaatttt gttggcgtaa
    10981 gggtaaaggc acggccctga tggtcacatg ctgcgcatcg gactggaaat caatacccaa
    11041 ttccgccagg gcagactgcg ctttttctaa tgcagatttt tcttcgccag aaacttttag
    11101 ccgcagcgga atcagcagcg gctgggcgca aacgggcgct tcacccggcg tcaattgtgc
    11161 ctgacgcagc caacgttctg ccactggcaa ggctaaaagt gaaatgttgc cgtcgagctc
    11221 aagcaacgca cagtcggaat ggacgatagt cagtacccga ccaaagcttt gactgttcgc
    11281 cgcaagtgca ggttcctgcg gttctggcgc ttttggtttt tgcatcggcg cgggcgtttg
    11341 caaaagctgg cgatacactt caccttgctg tttctggtaa cctggctgcg cattcggcca
    11401 gggggcagcc ggacgactgc ctgaggcggg cgcaggagag tagcgcggag ctaccggctc
    11461 acgaactgcc ggttcagcaa agtgattgcg ccccgccgcc acgcgatttt ccggaatggc
    11521 acgcggtgca ggttgtggtt catcgtccag cggtagcggt gtttccagtt gctgttgtag
    11581 cacgctcagc acgccctgat agataaagtc atgcaccaga cgcgactgat ggaaacgcac
    11641 ttcgtgtttg gcagggtgca cgttgacgtc cacctgatgc gggtcgatct ccagatacaa
    11701 cacaaatgcc ggttgctgat cggcccccag tttgtcttcg caggcctggc ggatcgcgtg
    11761 attgatcagg cgatcgcgca tcatgcgacc gttcacgtag caatactgaa tttcggccag
    11821 tgcgggcgta gtgtgatttg gatcggccac ccagccgcgc agcgtgagat cgccgtgttg
    11881 ccattcaatc gccagcgctt gttcgagaaa agcggtgccg caaatcgcgc ctaagcgccg
    11941 ttctttttgc ccgccttccg gcactgcgcg gtactggcgt acaattttgc cgttatgcga
    12001 caggttgata gtgacgtcga aacgcgccag cgcaatgcgg cggatgattt catcgatatg
    12061 gttaaattcg gttttctcgg tgcgcaggaa tttgcgccgc gccggggtgt tgtagaacag
    12121 gtccagcacc tccagcgtcg ttcccaccgg atgcgccgcc ggtttaaccg tcacgtccat
    12181 atcgcgccct tcggcatagg cctgccaggc ttcctgctgt tctgcggtgc gtgaagtgag
    12241 cgtcaggcgg gaaaccgaac tgatactcgc cagcgcctca ccgcgaaagc ccagactgat
    12301 aatggcttcg agatcgtcca gagaggcgat tttactggtg gcatgacgcg ccagcgccag
    12361 cgccagctcg tcttttttga taccgcagcc gttatcacga atgcgaataa gtttcgcccc
    12421 accgcgttcg atatcaatat cgatacgcgt cgcacctgca tcgaggctgt tttccaccag
    12481 ttctttgact accgacgcag gtcgctcgac cacctcacct gcggcaatct ggttcgccag
    12541 ttgtggcggt aagacctgaa ttggcatcaa tcgtccttag tttggcagcg tgcgatctgg
    12601 cgtcgtcacc gtactggccg tttgtgccgt tgcaccctgc ggcgcagatt gcatcggatg
    12661 cgcaaggaag taattgcgca ggcctttgta aatggcttct gccagctgtt gttggtaatc
    12721 gtcgctcgcc agcaaacgtt cttcgctgtt gttactgata aaaccggttt cgaccagtac
    12781 tgacggaata tccggcgaac gcagaacgcc gagactcgcg tgttctggtc ggcgtttatg
    12841 tatttcgcca atgcgttgca actgattgat catactggtc gctacatcat accctacccg
    12901 ctgggaatga ccgaactgta aatccagcac cgcctggctt aaataggggt cagactgact
    12961 gttcgccagc acatcgcccg ctccgcccag tagctccgac tgtttctcgt gctgctccag
    13021 ccagcttgcc atttcgctgt tggcgcgacg gttagagagc acccatacag aggcaccggt
    13081 ggcactacgg tttggtgcgg catcggcatg aatcgacaca aggaaattgg cgttttgctt
    13141 acgtgccaca tcactgcgcc ccatcaccga gataaaataa tccccgtcac gggttaaaac
    13201 gcccttaaac atcggatcgt tattgagcaa ggtacgtaat ttacgcgcaa tggcgatggt
    13261 gacatttttc tcccgtgtac caccagggcc gatagcgcca gggtcctgac cgccgtgtcc
    13321 ggcatcaata gcgatgataa ttttatcgcc tgtgttagcc gtcgcgcgcg ctgccggacg
    13381 cgttaccgta ttactgctga taacacccgt agtgcggtta ctttccgttt taaacggatt
    13441 gcgcgccggt tcgctgacgc gcggtgcgac aaccgcaggc gtttcaacgc gtttcgcaac
    13501 cattggcgga ggcgatggcg gcgcatcggc gttaatcgta aagacaacag tgtaatagct
    13561 gccattctgc cgcttcaccg cttcggtttt accgttttcg gtgagatcga ccaccagccg
    13621 tagcgtttgt gcatctttag gcgttccaga gcgaatcgcc ttcaccagat tattgccgct
    13681 aaacaacaac ggcagcccct gaattacccc cgtttgtttg atatcgagcg ccacggtgcg
    13741 tttgctttga tggctaaacg cataatcagg atcgccaata aaactcaacg ttatccgtgc
    13801 ctgttgatta ccgttagaaa cctgaatatc agagagcgtc gcggcaccca ccggcgcgca
    13861 cagcagcagc aacgtcgcta ccaaccaatt tctgatgcga tacatcatcc cgccaccttt
    13921 caaaggttaa ccggctaaac gcgccagcaa caattcaccc gcagaggaaa ccgcactcac
    13981 gcgcgcctca cggccttgtg cctgataatc aatgtgtatt tcgacatccg ggtcaggaag
    14041 aacacctgta ccttgttgtg gccactccac caggcagatg gcatcgttgg caaaataatc
    14101 gcggatcccc ataaactcca gctcctcggg atcggcaagg cggtacaaat caaagtgata
    14161 aaccagtaaa ttgtcgagcg tatagggttc gaccagcgta taagtggggc ttttgacatt
    14221 accctgatga cccagagcct gtaaaaagcc ccggctaaag gtggttttac ctgcacctaa
    14281 atcgccatac agatagatta cggttgcgcc atcgcaggct ttcgctaccc gctcgcccag
    14341 gtctaatgtt gcctgctcat cagggagcgg aattactcga ttcatcatgg tttttatcag
    14401 tcacttccgg gttaacaata cgctgtagcg tggaaaagag atcggttgcc agcatcccgc
    14461 gcgttccaaa acgcgccgcc agtacgtcag ctgccgcacc gtgcgcgaca cagcccgcac
    14521 aggctgcatc ataagggctc attttttgcc caagcaatgc gccaataata ccagagagca
    14581 catcgcccat gccgccgctc gccatgcctg catttccggc atcaataatg cctaaagcgt
    14641 caggatgggc ggcgaccacg gttccggcac ctttcagcac cgctacgccg ccataacgtt
    14701 gtaccagacg ttgggtgcaa tgtaagcggt cactttcaat ttcagcgacg gaacagccta
    14761 acaaccgtgc ggcctcgcca ggatgcggcg tgatcacgcg attgtgacgc ttatcgggat
    14821 tgattgccag caggttcaat gcatcggcat cccacaacat cggtttgcga aaattctcaa
    14881 ctttttgcag ggcttttttc ccccactctt gctggcccag accgggacca atcaccacca
    14941 catcggccca ttccaggctt tcggtaagag agtccatagt cagttcatgc accatcaatt
    15001 ccggtcgtgc agtcagcagc ggcgcaatgt tctcactgcg ggtcagtact cggactaaac
    15061 cagcaccagc acgcagcgcc gcttcccccg tcatacgaat agcccccgcc gtgccgtgat
    15121 cgccaccaat aatcaccagc cgcccgtgat cgcctttatg cgaagtcggg cgacgcggta
    15181 tcagccactg agaaagttgt tctgccgaaa accgctgaat tttcgtctcc tgacctgcca
    15241 gccagctatc cagccccagt gagtcaaaat gcagttgtcc ggtaacatcc cgcgcttttc
    15301 cagtgagcaa gcctggtttc agcgcaataa aagtgatggt gtgatcggcg ttgattaccg
    15361 cgcctggcgt agcgccggtt tcagccagaa ggccggaagg gatatcaacc gccacaatcg
    15421 gcgcagggtg agtattagcg tggtcgatta actggctaat ggattcgcgg ggcgcttgct
    15481 gcaagccggt gccgagcagc gcatcaacaa taagatctac cgattcgggc cagacaatat
    15541 tcgaagcatg gatctcgcct cccgcgttta accatgcttc gcgtgccagc gcggcctctt
    15601 ccggcaacgg tttgtcactt tcctgggcca gcagcgtgac atcaataccg atcgctttgg
    15661 ccagccgcgc gaccacgtag ccatcgccgc cgttattacc atgaccgcac agcaccagcc
    15721 agtggcgggc gtcaggataa gccgaacgac acacctggaa tgcggcttcg ccagcgcgaa
    15781 gcatcagctc atagagtgtg agccccagcg catctgccgc ctcgcgttct ccgcggcgga
    15841 tatcgtcggc ataccagacg gtgtgtggta tacttacggg gtttttcttc attgtatggt
    15901 ccgtcatgtc agagcccctc gatctcaatc aattagcgca aaatattaaa cagtgggggc
    15961 tggaactggg ctttcagcag gtaggtatta ccgataccga tctcagcgcg tccgagccca
    16021 aactgcaagc atggctggac aaacaatacc acggcgaaat ggactggatg gcgcgtcacg
    16081 gtatgctgcg tgctcgtcct cacgaattat tgcccggtac gctgcgcgtg atcagcgtgc
    16141 ggatgaatta ccttcctgct aacgccgcat ttgccagcac actgaaaaat cccaaactcg
    16201 gctatgttag ccgttatgcg ctgggccgtg actatcacaa gcttctgcgc aaccgactca
    16261 aaaagctggg cgagatgatt cagcaacatt gtgtttcgct gaattttaga ccgtttgtcg
    16321 attctgcgcc tattctcgag cgcccgttag ctgaaaaagc aggtcttggc tggacaggta
    16381 agcactcact tatcctcaat cgcgaggccg gttcgttctt ctttttaggc gaattgctgg
    16441 tcgatattcc gctgcccgtg gatcaaccag tcgaggaagg atgcggtaaa tgcgtggcct
    16501 gtatgacgat ttgcccgacc ggtgccatcg tcgagccata taccgtcgat gctcgccgct
    16561 gtatctctta tctcaccatc gaactggaag gggcgatccc ggaagagttg cgaccgttaa
    16621 tgggaaaccg tatttacggt tgcgatgact gccagcttat ctgcccgtgg aatcgctatt
    16681 cgcaactcac tacagaagac gatttcagcc cgcgtaagcc gctacacgca ccggaactca
    16741 ttgagttatt cgcctggagt gaagagaagt ttttaaaagt cacggaaggt tcggcgattc
    16801 gccgtatagg tcacctgcgt tggctgcgta atatcgccgt agcattaggc aatgccccct
    16861 gggatgaaac gattttgaca gcgctggaaa gtcgtaaagg tgagcaccca cttcttgatg
    16921 agcacatagc gtgggcgatt gcgcagcaaa tcgagagacg aaatgcgtgc atagtcgaag
    16981 tgcaattgcc gaaaaaacag cgtctggttc gggtgattga aaaagggtta ccgcgtgacg
    17041 cctgattcat ccacagcttg tgaataaaaa taaaaacgca ttgcaattca agacgaataa
    17101 aatcgtcaag tgatcgactc gacaaaacga aatcttaatt tttaacttat ctatcaatag
    17161 gttaaaaaaa tactattcac cgtgcgaagt ttctttgaaa ttaagattac ggcctgaggc
    17221 tgtggataac tctgtttaca agagtttgtc agagacaaca aaaaccagca ataacgcatc
    17281 gcgtcgctgt ggatatttta ttgagagaag aatttggagc gggaaacgag actcgaactc
    17341 gcgaccccga ccttggcaag gtcgtgctct accaactgag ctattcccgc ttgggtggtc
    17401 tgtgctttac agcactttca aattttggag cgggaaacga gactcgaact cgcgaccccg
    17461 accttggcaa ggtcgtgctc taccaactga gctattcccg cttaatcttc gtctttcgaa
    17521 ccgctactgc cttggctgcc ttcactcacc ttagtcactt acttttgtaa gctcccaagg
    17581 attcgttcag ttgccgcctt gtcgcaattc gaaatactgc gattgggtgg tctgtgcttt
    17641 acagcacttt tcaaattttg gagcgggaaa cgagactcga actcgcgacc ccgaccttgg
    17701 caaggtcgtg ctctaccaac tgagctattc ccgcgtacta cttaattttg ctttcgtaat
    17761 tttcgcattt ctgcgtcgtt acgggaggcg cattatacga gaaatctttc cctctgcaac
    17821 ccccctaaaa aatttttttt gagtttttat ctcaagtgct gaaattatca gcaagacgtt
    17881 caatttagcg tcaaaatcac gccgccgcaa gtggattccg gcgcttacgt gaccaggaaa
    17941 aattttacag cttgataaaa tgctcgcggt agtaagccaa ctccgccacc gattcacgga
    18001 tatcatccat cgcctgatgc gtcccctgct tggtaaagcc gtcaagaatt tccggcttcc
    18061 agcggcgcgc cagctctttc agggtgctaa catcgagata gcggtagtgg aagtaggctt
    18121 ccagctccgg catgtattta aacaggaagc gacggtcctg accgatgctg ttaccacaaa
    18181 tcggcgattt tcccgcaggc acccactgtt ttaagaattc gagcgttgcc agttcagctt
    18241 cacgatcgcc catcgtgctc gctttcacac gctctaccag cccgctggcg gtatgggtgc
    18301 gcacgttcca gtcatccatc agcgccagct gttcatcgga ctgatgtact gcaatggtcg
    18361 gcccttctgc cagaatattc aggttggcat cggtcaccag cgtggcaatc tcaataatgc
    18421 gatcgcgctc gggatccaga ccggtcatct caagatcgat ccaaatcagg ttgttttcat
    18481 tggcactcat gctattttcc acccattcac agtgactata ttgatccaca attgcgtgta
    18541 tcatagatgt tttgcccatc aggggcgacc aggagtctgt acgattgagt aaaaataaac
    18601 tctccaaagg ccagcagcgc cgcgtgaacg ccaatcacca gcgtcgtctt aaaacgtcta
    18661 aggagaagcc cgactacgac gacaatctgt ttggcgagcc tgatgaaggt atcgtcatca
    18721 gccgctttgg tatgcacgct gatgtggaat ccgccgatgg cgacgttcac cgctgcaata
    18781 ttcgccgtac catccgttcg ttggtgaccg gcgaccgcgt agtctggcgt ccgggtaaac
    18841 cagcggcgga aggcgtaaat gtcaaaggga tcgtggaagc ggtgcatgag cgcacctcag
    18901 tgttgacgcg cccggatttt tacgacggtg tgaaacctat tgctgccaac atcgaccaga
    18961 ttgtcattgt ctccgccatt ttgccggagc tgtcgctcaa tattatcgac cgttacctgg
    19021 tggcctgcga aaccttgcag attgagccga ttattgtgct caacaagata gacctgctgg
    19081 acgacgaagg catggagttc gtcaacgagc agatggatat ctaccgcaat atcggttatc
    19141 gcgtattgat ggtttccagt catactcagg atgggctgaa accgctggaa gaggcgttga
    19201 ccgggcgcat cagcattttt gccgggcagt ctggcgttgg caaatccagc ctgctgaacg
    19261 cattgctggg tctgcaaaaa gagatcctga ccaacgatgt ctctgacaac tcggggcttg
    19321 gtcagcacac cacgaccgcc gcacgactgt atcacttccc gcacggcggt gatgtgattg
    19381 attccccagg tgtgcgtgag tttggtctct ggcacctgga gccggaacaa atcactcagg
    19441 gctttgtcga attccatgac tatttaggtc tgtgtaaata tcgcgattgc aaacacgata
    19501 ccgatccggg ctgcgctatc cgggaagcgg ttgaggaagg gaaaatcgcg gaaacccgtt
    19561 tcgaaaacta tcaccgtatt ctggaaagca tggcgcaggt aaaaacgcgt aaaaactttt
    19621 ctgatacgga tgactgacaa ttaagctaac cgtcgttaga atcgtcccct ttttttcagg
    19681 atccggcatg taggccggat caggaacgac aaaacaatgg cctggaggct accttgttaa
    19741 attcatttaa actttcgcta cagtacattc tgccgaaact atggcttact cgcctggcgg
    19801 gttggggcgc aagcaagcgg gcaggatggc tgacaaaact ggttatcgat ctgttcgtta
    19861 aatactacaa ggtcgacatg aaagaggcgc aaaagccgga caccgccagc taccgcacct
    19921 ttaacgaatt ctttgtccgt ccgctgcgtg acgaagtacg cccaatcgat accgatccga
    19981 atgtactggt tatgcctgcc gatggcgtta tcagccagtt gggtaaaatc gaagaagata
    20041 aaatcctgca agccaaaggc cacaactaca gcctcgaagc cctgctggca ggcaactatc
    20101 tgatggcgga tctcttccgt aacggtacgt ttgtgaccac ttacctctcc ccacgtgact
    20161 accaccgcgt acacatgccg tgtaacggta ttctgcgtga aatgatctac gtgccgggcg
    20221 atctcttctc cgttaaccat ctcacggctc agaacgtgcc gaatctgttt gcccgtaacg
    20281 aacgcgtgat ctgcctgttc gataccgaat ttggcccgat ggcgcagatt ctggtcggtg
    20341 cgacgattgt aggcagcatt gagacggtct gggcgggcac cattacgccg ccgcgcgaag
    20401 gcatcatcaa gcgctggacc tggcctgccg gggaaaacga cggttctgtg gcactgctga
    20461 aaggccagga aatgggtcgc tttaaactag gctctaccgt tatcaacctg tttgcaccag
    20521 gtaaagtgga cctggttgag caactggaaa gcctgtctgt cacgaaaatt ggccagccgc
    20581 tggcagtatc taccgaaacc tttgttacgc cagatgctga accagccccg cttcctgctg
    20641 aagaaatcga ggcagaacac gacgccagcc cattggttga cgataaaaaa gaccaggtct
    20701 aatccatcaa aggaaacgct gacgtgcgcc tgattatcac ttttctgatg gcctggtgcc
    20761 tcagttgggg ggcgtacgcc gcgacggccc ccgatagcaa acaaatcacc caggaactgg
    20821 agcaggcaaa agcggcgaaa cctgcgcagc cggaagtcgt agaggcgctc cagtctgcct
    20881 taaatgcgct tgaggaacga aaaggttccc ttgatcgcat caagcaatat caagaagtca
    20941 ttgataatta tccgaaactt tccgccactc tgcgcgcgca gttaaacaac atgcgtgacg
    21001 agccacgcag cgtgtcgccg ggaatgtcta acgatgcgct gaatcaggaa attctccaga
    21061 tcagcagtca gttgctggat aaaagccgtc aggcccagca agagcaggag cgcgcccgcg
    21121 agattgccga ttcgctgaat caactgccgc aacagcaaac cgatgcccgc cgtcagttga
    21181 atgaaatcga gcgccgcctg gggacgctta ccggcaatac cccgctcaat caggcacaaa
    21241 atttcgcgtt gcagtctgac tccgcacgtc tgaaagcgct cgttgatgaa ctggagctgg
    21301 cgcagctatc tgccaataac cgccaggaat tagcgcgctt gcgctctgag ctggcggaaa
    21361 aagagagcca gcagctggat gcatatttgc aagcactacg caaccagttg aatagtcaac
    21421 gccaactgga agcggagcgg gcgctggaaa gcaccgaatt gctggcagaa aacagtgccg
    21481 atttgcctaa agacatcgtc gcgcaattca aaattaaccg cgaactatcg gcggcgctga
    21541 atcaacaggc acagcggatg gatctcgttg cctcgcaaca gcgtcaggct gccagccaga
    21601 cattacaggt ccggcaggcg ttgaatactc tgcgtgaaca gtcgcaatgg ctgggatcat
    21661 ctaacctgct cggcgaagcg ttgcgggcgc aggtggcacg gctaccggaa atgccaaaac
    21721 cacaacagct tgataccgaa atggcgcagt tacgtgtgca acggttacgt tatgaggatc
    21781 tgcttaataa acagccgctg ctacggcaaa tccatcaggc cgacggtcag ccgctgaccg
    21841 ctgagcaaaa ccgtattctg gaagcacagc tacgcactca gcgtgagttg ctgaactcat
    21901 tgttgcaggg tggcgacacg ctactgctgg agctgaccaa gctgaaagtc tccaacgggc
    21961 aactggagga tgcgctgaaa gaggtgaacg aagcgacgca ccgctatctg ttctggacct
    22021 ctgacgtgcg cccgatgacc atcgcctggc cgctggaaat cgcccaggat ctgcgtcgtc
    22081 tcatttcgct ggacaccttc agtcagttgg gcaaagccag tgtgatgatg ctgaccagta
    22141 aggagacgat tttgccgctg tttggcgcgt tgattctggt cggttgcagt atttactcgc
    22201 gccgctattt cacccgtttt cttgaacgtt cggcggcgaa agtcggcaaa gtgactcagg
    22261 atcacttctg gctgacgtta cgcactcttt tctggtcgat tctcgtcgcg tcaccgctgc
    22321 ctgtgctgtg gatgacactg ggttacggct tgcgcgaggc gtggccttat ccgctggcgg
    22381 tcgcgattgg tgatggcgta acagccaccg tgccgctgct gtgggtagtg atgatttgcg
    22441 ccacctttgc tcgcccgaac ggcttgttta tcgctcattt tggctggccg cgcgaacgag
    22501 tttcccgtgg gatgcgttac tacctgatga gcatcgggct cattgtgccg ctgattatgg
    22561 cgctgatgat gttcgataac ctcgacgacc gtgaattctc cggttcgctg ggacggcttt
    22621 gctttatcct catttgcggt gcgctggcgg tggtcaccct cagcctgaaa aaggccggga
    22681 tcccgctgta tctcaacaaa gagggcagcg gcgacaacat taccaaccat atgctgtgga
    22741 acatgatgat tggcgcaccg ctggttgcca ttctggcgtc ggcggtgggt tatctggcaa
    22801 cggcgcaggc gctgttagcg aggcttgaaa cctcggttgc catctggttt ttgctactgg
    22861 tggtttatca cgttatccgc cgctggatgc tgatccagcg ccgcaggctg gcgtttgacc
    22921 gggcgaagca tcgccgggca gagatgttag cgcaacgtgc gcgtggcgaa gaggaagcgc
    22981 atcatcacag tagcccggaa ggagcaattg aagtcgatga aagcgaagtc gatctcgatg
    23041 ccatcagtgc gcaatccttg cggctggtgc gctcaatttt gatgttgatc gccctgcttt
    23101 cggtcattgt gctgtggtca gaaatccatt ccgctttcgg cttcctcgaa aatatttcgc
    23161 tgtgggatgt cacttccacg gtacagggcg tagaaagtct ggagccgatt accctcggtg
    23221 cggtgctgat tgccattctg gtgtttatca tcaccacgca gctggtgcgc aacctgcccg
    23281 cactgctgga actggcgatt ttgcagcacc tggatttaac gccgggtacg ggctacgcca
    23341 tcaccaccat caccaaatat ctgctgatgc tgattggcgg gctggtcggc ttctcaatga
    23401 ttggtattga gtggtcgaaa ttgcagtggc tggttgccgc gctcggtgtt ggtctcggtt
    23461 ttggtttgca ggaaattttc gccaacttta tctctggcct gatcatcctg ttcgaaaaac
    23521 cgattcgcat tggcgatacg gtaacgatcc gcgatcttac cggtagtgtg acgaaaatta
    23581 acacccgcgc caccaccatc agcgactggg accgcaaaga aattatcgtg ccgaacaagg
    23641 cgtttattac cgagcagttt atcaactggt cgctctctga ctcggtcacg cgtgtggtgt
    23701 tgacaattcc ggcccctgcc gatgccaaca gtgaagaagt gacggaaatc ctgctcaccg
    23761 cagcgcgtcg ctgctcactg gtgatcgaca acccagcacc ggaagtcttc ctggtggatc
    23821 tgcagcaggg gattcagatt ttcgagctgc gtatttacgc cgctgagatg ggtcaccgta
    23881 tgccgctacg ccatgagatc caccaactaa ttctggcggg cttccacgcc cacggtatcg
    23941 atatgccatt cccgcccttc cagatgcgtc tggaaagcct caacggtaaa caaacgggga
    24001 gaacattaac ttctgcgggc aaaggtcgtc aggcgggaag tttgtaatgc atgaagctgc
    24061 caaatgatgt caacgtggct catttagcag cttaacatca tcttcagtta tcactggccc
    24121 cggagagtat catccatacg cattacttcc tgacaactca tcgccggaga gacgatgagt
    24181 tgcaggattt catatgatca gtcgagagct aactcaggtt tgttcgccat gcgactgcaa
    24241 tagttctggt aaatcgccat cgctaacagc gagaagaaga cagggccgcc aatcatccac
    24301 aatgtgctgt cccagtctcc agcttcaacc acaggttgaa tgatggtgaa gacgttcgca
    24361 aatgtcacca ccagtacgac aaccactgtt gcaatcattg ccgacatatg cgttttgaaa
    24421 atcacaaacg gtctgtcgag atcctgacgt gctttaaaaa atgggaaagc cagcgcgagg
    24481 aacaggtaag gaagcgtcat agacacgttc gccatcagcg tcagcttgtt aaagaacgcc
    24541 gatgcggtac cgccaccaaa cgaaaccagc agaatgaaga tagtaaccaa cccgcactgc
    24601 atccacatgg caatagacgg catccccatc gtattcaggc gcgtcatcgg ttccggccac
    24661 aatgctttcg gcgttccctg gatgatggct ttcagcggtg aatagcaaag cgtaaagaac
    24721 gcaccggtat aagcgaggaa catcgaaagt ccggtaatac gcgcaaacca tacgcccagc
    24781 gacaatgacg cttcaggtga aaaatgcagt gcgttaccca gcgtcacccc gaggctcttc
    24841 atcagcacat aggtaatatt gccgaggtta acggaaccat tacttaatac ctgctgccag
    24901 tttgtgctga cgccccataa aaatattgcc agcgaataac cgattgaaat aacaatagcg
    24961 gcaaaaacaa tacctttggc aaagttcttt tctggatttt ccgttttatc aaccagacca
    25021 ccaaccgctt caattccgcc ataggcaaaa atggcaaata ccacaaacga tagcattgcc
    25081 agaccggact gataacctgg gttcggtgat gcaaggaaat taatatcctg cgcgaaatgc
    25141 ccaccattta ataacaaaat agtaatgctt actaacagca atactaaatt cagacacatt
    25201 actgcaatac cgcccaccgc agtaatgcgg gcaattttat taatcccttt tgaagcaacg
    25261 acggtgacca gaatcatcca tgccactgcc agcagaccaa ccacctgcgt aggctccagt
    25321 ccggcaatat gccagtgctg ggtcatgtcg ctaccgtaga ggaatgttga gaacggtacc
    25381 caaactttcg cggaggtact caccatccag atgatataag aggaaaacca cataaacgta
    25441 ccaatgaagg caaaacgtgg tccgacacta ttattcatcc aggaatagat accgccttct
    25501 tctttgcgat aagcagctcc catttcagcc atcattaagg cgaatggaat aaagaataac
    25561 aatgcagaaa atatataaaa gggaatcgca ctataaccca ttaagtaata agccgatggg
    25621 ctattggcaa atccaaatac agaagtaaag atcatcaata tgagtcctat cagactcatc
    25681 ttttttatcg agtgaggcat gaaaccatcc ttcatttcag cgcgtgtata aaacgcgccc
    25741 actgaactgc actgcccaac attgagcggg cagcgtaaaa accaatacag aattgatacc
    25801 tggatgatat caaatatcca gtcttaaatt gtggccttta gcggaaaaat gaaaacatat
    25861 gctacagaat ggcgcttatc acgccattct tcgctgttaa ttcagtaatt tttcagaatt
    25921 atgcccggtc aacgctaaag gcgatgactt cagccagtgt ctccgcgccc agcgccaaca
    25981 tcaccagacg atcaacacct aatgccacgc cggaacagtc aggcataccg actttcaagg
    26041 cttcaatcag attctggtca atggggtgct gcggcaaacc gcgcgccgcg cgcttacggt
    26101 tatcttgttc aaagcgttgt tgctgctcac gggcatccgt caattcatgg aaaccattcg
    26161 ccagctcaat acctttataa taaacctcaa agcgttcagc gacccgatga tcttcggtac
    26221 tgatttgcgc cagtgatgcc tggctggctg gaaagtggta cacaaaggtc ggtttttctt
    26281 tgccaatatt tggctctacg ccaaaggtaa acagcaattg tagaagcgtg tcgcggtctt
    26341 cttcagtatc ggcaacgttg ctcaaatcca gtttcgctgc cacttcccgc agttgcgttt
    26401 tgtcggcaga gagtgggtca atttccagat aacgcaagaa agcttgttga taagaaaggc
    26461 tttctgctgc cggacagtcc agcacctgtt gcaagagatc gtccacctcg ttcatcaacc
    26521 ggtacatatc atagtgcggt cgataccact caagcatagt gaactcaggg ttgtgataac
    26581 gccccatctc ttcattacgg aagctgcggc acagctggaa taccggccca caaccggcaa
    26641 ccagcaggcg cttcatatgg tattccgggc tggtcattaa ccagagattc atcccctgcg
    26701 aatgcccggg gccaacgaaa cgtgtctcaa acgggaccaa atgaatatcg gttaccgtcg
    26761 cctggctcat acaaggcgtc tccacctcca gcactccacg atcggcaaag aaacgacgga
    26821 tctccgccat aatcgccgcg cgttttaata agttaggaat ggatgcgctc ggctgccagg
    26881 atgccgtttc gctcatagtt aaatctccag tttttgacaa gggcacgaag tctactcgca
    26941 acgcgacggc gagacaaatt ttacgcagga ataacacaac ggtgggcagt gactaaaaaa
    27001 agcacgatct gatggtttag taattaaatt aatcatcttc agtgataatt tagccctctt
    27061 gcgcactaaa aaaatcgatc tcgtcaaatt tcagacttat ccatcagact atactgttgt
    27121 acctataaag gagcagtgga atagcgttcg cagaccgtaa ctttcaggca cttaccctga
    27181 agtacggggc tgtgggataa aaacaatctg gaggaatgtc gtgcaaacct ttcaagccga
    27241 tcttgccatt gtaggcgccg gtggcgcggg attacgtgct gcaattgctg ccgcgcaggc
    27301 aaatccaaat gcaaaaatcg cactaatctc aaaagtatac ccgatgcgta gccataccgt
    27361 tgctgcagaa gggggctccg ccgctgtcgc gcaggatcat gacagcttcg aatatcactt
    27421 tcacgataca gtagcgggtg gcgactggtt gtgtgagcag gatgtcgtgg attatttcgt
    27481 ccaccactgc ccaaccgaaa tgacccaact ggaactgtgg gggtgcccat ggagccgtcg
    27541 cccggatggt agcgtcaacg tacgtcgctt cggcggcatg aaaatcgagc gcacctggtt
    27601 cgccgccgat aagaccggct tccatatgct gcacacgctg ttccagacct ctctgcaatt
    27661 cccgcagatc cagcgttttg acgaacattt cgtgctggat attctggttg atgatggtca
    27721 tgttcgcggc ctggtagcaa tgaacatgat ggaaggcacg ctggtgcaga tccgtgctaa
    27781 cgcggtcgtt atggctaccg gcggtgcggg tcgcgtttat cgttacaaca ccaacggcgg
    27841 catcgttacc ggtgacggta tgggtatggc gctaagccac ggcgttccgc tgcgtgacat
    27901 ggaattcgtt cagtatcacc caaccggcct accaggttcc ggtatcctga tgaccgaagg
    27961 ctgccgcggt gaaggtggta ttctggtcaa caaaaatggc taccgttatc tgcaagatta
    28021 cggcatgggc ccggaaactc cgctgggcga gccgaaaaac aaatatatgg aactgggtcc
    28081 acgcgacaaa gtttctcagg ccttctggca cgaatggcgt aaaggcaaca ccatctccac
    28141 gccacgtggc gatgtggttt acctcgacct gcgtcacctc ggcgagaaaa aactgcatga
    28201 acgtctgccg ttcatctgcg aactggcgaa agcgtacgtt ggcgtcgatc cggttaaaga
    28261 accgattccg gtacgtccga ccgcacacta caccatgggc ggtatcgaaa ccgatcagaa
    28321 ctgtgaaacc cgcattaaag gtctgttcgc cgtgggtgaa tgttcctctg ttggtctgca
    28381 cggtgcgaac cgtctgggct ccaactcgct ggcggaactg gtggtcttcg gtcgtctggc
    28441 cggtgaacaa gcgacagagc gtgcagcaac tgccggtaat ggcaacgaag cggcaattga
    28501 agcgcaggca gctggcgttg aacaacgtct gaaagatctg gttaaccagg atggcggcga
    28561 aaactgggct aagatccgcg acgaaatggg catggccatg gaagaaggct gcggtatcta
    28621 ccgtacgccg gaactgatgc agaaaaccat cgacaagctg gcagagctgc aggaacgctt
    28681 caagcgcgtg cgcatcaccg acacttccag cgtgttcaac accgacctgc tctacaccat
    28741 tgagctgggc cacggtctga acgttgctga atgtatggcg cactccgcaa tggcacgtaa
    28801 agagtcccgc ggcgcgcacc agcgtctgga cgaaggttgc accgagcgtg acgacgtcaa
    28861 cttcctcaaa cacaccctcg ccttccgcga tgctgatggc acgactcgcc tggagtacag
    28921 cgacgtgaag attactacgc tgccgccagc taaacgtgtt tacggtggcg aagcggatgc
    28981 agccgataag gcggaagcag ccaataagaa ggagaaggcg aatggctgag atgaaaaacc
    29041 tgaaaattga ggtggtgcgc tataacccgg aagtcgatac cgcaccgcat agcgcattct
    29101 atgaagtgcc ttatgacgca actacctcat tactggatgc gctgggctac atcaaagaca
    29161 acctggcacc ggacctgagc taccgctggt cctgccgtat ggcgatttgt ggctcctgcg
    29221 gcatgatggt taacaacgtg ccaaaactgg catgtaaaac cttcctgcgt gattacaccg
    29281 acggtatgaa ggttgaagcg ttagctaact tcccgattga acgcgatctg gtggtcgata
    29341 tgactcactt catcgaaagt ctggaagcga tcaaaccgta catcatcggc aactcccgca
    29401 ccgcggatca gggtactaac atccagaccc cggcgcagat ggcgaagtat caccagttct
    29461 ccggttgcat caactgtggt ctgtgctacg ccgcgtgccc gcagtttggc ctgaacccag
    29521 agttcatcgg tccggctgcc attacgctgg cgcatcgtta taacgaagat agccgcgacc
    29581 acggtaagaa ggagcgtatg gcgcagttga acagccagaa cggcgtatgg agctgtactt
    29641 tcgtgggcta ctgctccgaa gtctgcccga aacacgtcga tccggctgcg gccattcagc
    29701 agggcaaagt agaaagttcg aaagactttc ttatcgcgac cctgaaacca cgctaaggag
    29761 tgcaacatga cgactaaacg taaaccgtat gtacggccaa tgacgtccac ctggtggaaa
    29821 aaattgccgt tttatcgctt ttacatgctg cgcgaaggca cggcggttcc ggctgtgtgg
    29881 ttcagcattg aactgatttt cgggctgttt gccctgaaaa atggcccgga agcctgggcg
    29941 ggattcgtcg actttttaca aaacccggtt atcatgatca ttaacctgat cactctggcg
    30001 gcagccctgc tgcacaccaa aacctggttt gagctggcac caaaagcggc caatatcatt
    30061 gtaaaagacg aaaaaatggg accagagcca attatcaaaa gtctctgggc ggtaactgtg
    30121 gttgccacca tcgtaatcct gtttgttgcc ctgtactggt aaggagcctg agatgattaa
    30181 tccaaatcca aagcgttctg acgaaccggt attctggggc ctcttcgggg ccggtggtat
    30241 gtggagcgcc atcattgcgc cggtgatgat cctgctggtt ggtattctgc tgccgctagg
    30301 gctgtttccg ggtgatgcac tgagctacga gcgcgttctg gcgttcgcgc agagcttcat
    30361 tggtcgcgta ttcctgttcc tgatgatcgt tctgccgctg tggtgtggtt tacaccgtat
    30421 gcaccacgcg atgcacgatc tgaaaatcca cgtacctgcg ggtaaatggg ttttctacgg
    30481 tctggctgct atcctgacag ttgtcacgct gattggtgtc gttacaatct aacgcatcgc
    30541 caatgtaaat ccggcccgcc tatggcgggc cgttttgtat ggaaaccaga ccttatgctc
    30601 aaaacgacgc tctgcacctt attaattacc gcctcttgct ccacatttgc cgcccctcaa
    30661 caaatcaacg atattgtgca tcgcacaatt accccgctta tagagcaaca aaagatcccc
    30721 ggtatggcgg tggcggtaat ttatcagggt aaaccttatt actttacctg gggctatgcg
    30781 gacatcgcca aaaagcagcc cgtcacacag caaacgttgt ttgagttagg ttcggtcagc
    30841 aaaacattta cgggcgtgct tggtggcgac gctattgctc gaggggaaat caagttaagc
    30901 gatcccacaa caaaatactg gcctgaactt accgctaaac agtggaatgg gatcacacta
    30961 ttacatctcg caacctacac cgctggcggc ctgccattgc aggtgccgga tgaggtgaaa
    31021 tcctcaagcg acttgctgcg cttctatcaa aactggcagc ctgcatgggc tccaggaaca
    31081 caacgtctgt atgccaactc cagtatcggt ttgttcggcg cactggctgt gaagccgtct
    31141 ggtttgagtt ttgagcaggc gatgaaaact cgtgtcttcc agccactcaa actcaatcat
    31201 acgtggatta atgtaccgtc cgcagaagaa aagaactacg cctggggata tcgcgaaggt
    31261 aaggcagtac atgtttcgcc aggggcgtta gatgctgaag cttatggtgt gaagtcgacc
    31321 attgaagata tggcccgctg ggtgcaaagc aatttaaaac cccttgatat caatgagaaa
    31381 acacttcaac aagggataca actggcacaa tctcgctact ggcaaaccgg cgatatgtat
    31441 cagggtctgg gctgggaaat gctggactgg ccggtaaatc ctgacatcat cattaacggc
    31501 agtgacaata aaattgcact ggcagcacgc cccgtaaaac cgattacgcc cccaactcct
    31561 gcagtacgcg catcatgggt acataaaacg ggggcgaccg gcggatttgg tagctatgtc
    31621 gcgtttattc cagaaaaaga gctgggtatc gtgatgctgg ctaacaaaaa ctaccccaat
    31681 ccagcgagag tcgccgccgc ctggcagatt ctcaacgctc tacagtaaaa tttcatcggg
    31741 tccgaatttt cggacctttt ctccgctttt ccttgctgtc atctacactt agaaaaaaac
    31801 cagtaaggaa acatttatgc gcctgctccc tctcgttgcc gcagcgacag ctgcatttct
    31861 ggtcgttgcc tgcagttccc ctacgccacc gcgtggcgtg accgtagtga ataatttcga
    31921 cgctaaacgt tatctcggta cctggtatga gattgcccgt tttgatcacc gctttgaacg
    31981 tggactggaa aaagtcaccg caacatacag cctgcgtgat gacggcggcc tgaatgtcat
    32041 taataaaggc tataaccctg acagagaaat gtggcagcag agtgaaggga aagcgtactt
    32101 taccggcgca ccaactcgcg ctgcgctgaa agtgtcattc tttggtcctt tctatggcgg
    32161 ttataacgtt attgcactcg atcgggaata ccgccatgcg ctggtttgcg gcccggaccg
    32221 cgactacctg tggatactct cccgcacgcc aaccatttct gacgaagtga aacaggagat
    32281 gctggcagtc gcgacccggg aagggtttga tgtcagtaag tttatttggg tacagcagcc
    32341 cggtagttaa tgagtgctga gtttcagacc aataatcccc aatacgatta acgccagact
    32401 cgccaggcgc atcgggttag cggactcacc gagcagcaca atgccggtga tggccgcgcc
    32461 gactgcgcca atacccgtcc acacggcata agccgtccct actggtaacg atttcatcgc
    32521 ccaggcaagt agcgccagac tgacaatcat cgccgtcacg gtaataacac ttggcgtcaa
    32581 acgactaaag ccgtgagtat atttcaggcc aacggcccat accacttcca gcagaccagc
    32641 aataactaag ataatccagg acatatcagg ctccggaaaa ttggggccgt ccccggtgaa
    32701 aagacgcgtt tgcaggtcgt cctgcaaagc tgatggttga acggatattt tgccaggtgg
    32761 caggggaatt tcaactatta cctatgtaac tatcgcagaa aaaacaaacg gcacgacaca
    32821 gctgtcgtac cgttattgct tattgctgcg ctttcgttgc tgcgccagaa atcgcgttac
    32881 cgccatcaga aatgtcttca ccaacgccac gcgtggtgtt gcaggcagtt aatactgttg
    32941 aaagcaccag aacagaaaag atcgctgcaa ttgtcttctt caccataacg tcttcctttt
    33001 aggtaatgtt atttatgttt gcctatagca aattaataat agacaacatc gccaaaaatg
    33061 acggaaatct gatgattttt cggaagagaa gaaaaattag ctggcagcgc gggagatgga
    33121 gttgccgaga tgtttgatat cttcgccgaa accgcgagcg gtgttacagc ccgtgagcag
    33181 cgcgctggca agcaaaacaa gaacgataag gcgtttcatc atctctgacc tgaaaaagtg
    33241 atggtgcagc ctgcaggccg caccacaacc gcattacttc acgcgagaga cgtattcacc
    33301 agagcgggta tccactttga tgacttcgcc gatctgtacg aacagcggaa ctttaaccac
    33361 agcgccagta gacagggtag ccggtttgcc gccagtacct gcggtatcac ctttcaggcc
    33421 cggatcggta tcaacgattt ccagttcaac gaagttcggc ggagtaacgg agatcggctg
    33481 accattccac agagttacga tacactctgc ctgatccagc aaccatttag cgttgtcgcc
    33541 aattgctttt gcatcagcag acagctgctc gaaagtttcg ttgttcatga agtgccagaa
    33601 ctcaccgtcg ttgtacaggt aagtcaggtt catatcgaca acatcagcgc cttcagcgga
    33661 atcagtagat ttgaaggttt tttctacgcg agtaccggtc agcagacgac gcagtttaac
    33721 gcgagcaaat gcctggcctt tacccggttt tacgaattca ctcgcttcaa ccgcgtaagg
    33781 ttcgccgtct aacatgattt taagaccagc acgaaaatcg ttgctatagt acgttgccat
    33841 aaggccctct gaaatttgtt aattggtagc taagccacaa aatggcgcat attgtaaccc
    33901 taaatacccc atccagagaa gattggttaa cgcaacttgc cgatgttgtg accgatcctg
    33961 atgaacttct gcgtcttttg aatatagacg cggacgaaaa actgttggcc ggacgcagcg
    34021 ccaaaaagct gtttgccctg cgtgtgcccc gctcatttat cgatcgcatg gagaaaggca
    34081 atccggacga tcctcttttg cgtcaggtac ttacctcgca agatgagttt gtcgtcgcgc
    34141 ccggattctc caccgacccg ctggaagaac agcatagcgt agtgcctggt ttgttgcata
    34201 aataccacaa ccgggcgctt ttgctggtca aaggcggctg cgcggtaaat tgccgctatt
    34261 gcttccgtcg ccacttcccc tatgccgaaa atcagggcaa taagcgtaac tggcagactg
    34321 cacttgagta tgttgctgcg catccggaac tggacgagat gattttctcc ggcggcgacc
    34381 cgctgatggc gaaagatcac gagctggact ggttgctcac acaactggaa gccatcccgc
    34441 atatcaaacg tctgcgaatt cacagccgtc tgccgattgt gatcccggca cgcatcacag
    34501 atgcgctggt tgaacgcttt tctcattcta cgctgcaaat cttgctggtg aatcacatta
    34561 accatgccaa tgaggtagat gaaacattcc gtcaggcgat ggctaagttg cgccgggtgg
    34621 gcgttacttt gctgaaccag agcgttctgt tacgtggtgt gaacgataac gcgcaaacgc
    34681 tggcaaacct gagtaatgcg ttgttcgatg cgggcgtgat gccgtattac ctgcatgtgc
    34741 tcgataaagt acagggcgcg gcgcatttta tggtgagtga tgacgaagca cggcagatta
    34801 tgcgtgagtt gctgacactg gtgtcgggat atctggtgcc taaactggcg cgagaaattg
    34861 gcggcgaacc cagcaaaacg ccgctggatc tccagctacg ccagcagtaa ctgattaaac
    34921 agggaagttt aatctttgcc cgttgcataa cctttaatta ttcatataca ttgctgttgt
    34981 tactctttat ataaccaa
//
LOCUS       DAZFJL010000033        34894 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFJL010000033 DAZFJL010000000
VERSION     DAZFJL010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.033"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            77..1732
                     /gene="aslA"
                     /locus_tag="ACHJ8C_001627"
     CDS             77..1732
                     /gene="aslA"
                     /locus_tag="ACHJ8C_001627"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFO5551498.1"
                     /translation="MEFSFSPKLLVVAVAAALPLIASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYITQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1823..3058)
                     /gene="aslB"
                     /locus_tag="ACHJ8C_001628"
     CDS             complement(1823..3058)
                     /gene="aslB"
                     /locus_tag="ACHJ8C_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO5551499.1"
                     /translation="MQQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLDFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGVEFIQFIPVVERLADETAAHAGLKLH
                     APGDIQGELTEWSVCPQEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMLQQTIAEMIDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(3205..3281)
                     /locus_tag="ACHJ8C_001629"
     tRNA            complement(3205..3281)
                     /locus_tag="ACHJ8C_001629"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3245..3247),aa:Pro,seq:tgg)
     gene            complement(3324..3410)
                     /locus_tag="ACHJ8C_001630"
     tRNA            complement(3324..3410)
                     /locus_tag="ACHJ8C_001630"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3374..3376),aa:Leu,seq:cag)
     gene            complement(3431..3506)
                     /locus_tag="ACHJ8C_001631"
     tRNA            complement(3431..3506)
                     /locus_tag="ACHJ8C_001631"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3471..3473),aa:His,seq:gtg)
     gene            complement(3565..3641)
                     /locus_tag="ACHJ8C_001632"
     tRNA            complement(3565..3641)
                     /locus_tag="ACHJ8C_001632"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3605..3607),aa:Arg,seq:ccg)
     gene            complement(3744..5129)
                     /gene="yifK"
                     /locus_tag="ACHJ8C_001633"
     CDS             complement(3744..5129)
                     /gene="yifK"
                     /locus_tag="ACHJ8C_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5551500.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLVVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(5320..6060)
                     /gene="wecG"
                     /locus_tag="ACHJ8C_001634"
     CDS             complement(5320..6060)
                     /gene="wecG"
                     /locus_tag="ACHJ8C_001634"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO5551501.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6063..7415)
                     /gene="wzyE"
                     /locus_tag="ACHJ8C_001635"
     CDS             complement(6063..7415)
                     /gene="wzyE"
                     /locus_tag="ACHJ8C_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO5551502.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADAPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7412..8491)
                     /gene="rffT"
                     /locus_tag="ACHJ8C_001636"
                     /gene_synonym="wecF"
     CDS             complement(7412..8491)
                     /gene="rffT"
                     /locus_tag="ACHJ8C_001636"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO5551503.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSGHAREFMVAGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPKLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8488..9738)
                     /gene="wzxE"
                     /locus_tag="ACHJ8C_001637"
     CDS             complement(8488..9738)
                     /gene="wzxE"
                     /locus_tag="ACHJ8C_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO5551504.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAVIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9740..10870)
                     /gene="rffA"
                     /locus_tag="ACHJ8C_001638"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9740..10870)
                     /gene="rffA"
                     /locus_tag="ACHJ8C_001638"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO5551505.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAAERINQQRLALWQNYYDALAPLAEAGR
                     IELPSIPDDCVQNAHMFYIKLRDIDDRSALISFLKEAEIMAVFHYIPLHASPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(10875..11549)
                     /gene="rffC"
                     /locus_tag="ACHJ8C_001639"
                     /gene_synonym="wecD"
     CDS             complement(10875..11549)
                     /gene="rffC"
                     /locus_tag="ACHJ8C_001639"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO5551506.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGLGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11527..12408)
                     /gene="rfbA"
                     /locus_tag="ACHJ8C_001640"
     CDS             complement(11527..12408)
                     /gene="rfbA"
                     /locus_tag="ACHJ8C_001640"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO5551507.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12427..13494)
                     /gene="rffG"
                     /locus_tag="ACHJ8C_001641"
     CDS             complement(12427..13494)
                     /gene="rffG"
                     /locus_tag="ACHJ8C_001641"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO5551508.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEYQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWTALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     EIWWKQVQDGSYQGERLGLKG"
     gene            complement(13494..14756)
                     /gene="wecC"
                     /locus_tag="ACHJ8C_001642"
     CDS             complement(13494..14756)
                     /gene="wecC"
                     /locus_tag="ACHJ8C_001642"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO5551509.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14753..15883)
                     /gene="wecB"
                     /locus_tag="ACHJ8C_001643"
     CDS             complement(14753..15883)
                     /gene="wecB"
                     /locus_tag="ACHJ8C_001643"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO5551510.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(15939..16985)
                     /gene="wzzE"
                     /locus_tag="ACHJ8C_001644"
     CDS             complement(15939..16985)
                     /gene="wzzE"
                     /locus_tag="ACHJ8C_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO5551511.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(16997..18100)
                     /gene="wecA"
                     /locus_tag="ACHJ8C_001645"
     CDS             complement(16997..18100)
                     /gene="wecA"
                     /locus_tag="ACHJ8C_001645"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO5551512.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18340..19599)
                     /gene="rho"
                     /locus_tag="ACHJ8C_001646"
     CDS             complement(18340..19599)
                     /gene="rho"
                     /locus_tag="ACHJ8C_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO5551513.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19684..19785)
                     /gene="rhoL"
                     /locus_tag="ACHJ8C_001647"
     CDS             complement(19684..19785)
                     /gene="rhoL"
                     /locus_tag="ACHJ8C_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO5551514.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(19926..20255)
                     /gene="trxA"
                     /locus_tag="ACHJ8C_001648"
     CDS             complement(19926..20255)
                     /gene="trxA"
                     /locus_tag="ACHJ8C_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO5551515.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20386..21642
                     /gene="rhlB"
                     /locus_tag="ACHJ8C_001649"
     CDS             20386..21642
                     /gene="rhlB"
                     /locus_tag="ACHJ8C_001649"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO5551516.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMPK
                     PLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21778..23262
                     /gene="gppA"
                     /locus_tag="ACHJ8C_001650"
     CDS             21778..23262
                     /gene="gppA"
                     /locus_tag="ACHJ8C_001650"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO5551517.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(23309..25330)
                     /gene="rep"
                     /locus_tag="ACHJ8C_001651"
     CDS             complement(23309..25330)
                     /gene="rep"
                     /locus_tag="ACHJ8C_001651"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO5551518.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGAELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25417..25698
                     /gene="ppiC"
                     /locus_tag="ACHJ8C_001652"
     CDS             25417..25698
                     /gene="ppiC"
                     /locus_tag="ACHJ8C_001652"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO5551519.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25744..27219)
                     /gene="ilvC"
                     /locus_tag="ACHJ8C_001653"
     CDS             complement(25744..27219)
                     /gene="ilvC"
                     /locus_tag="ACHJ8C_001653"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO5551520.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     LREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            27369..28259
                     /gene="ilvY"
                     /locus_tag="ACHJ8C_001654"
     CDS             27369..28259
                     /gene="ilvY"
                     /locus_tag="ACHJ8C_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO5551521.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEDDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSAEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCV
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(28256..29800)
                     /gene="ilvA"
                     /locus_tag="ACHJ8C_001655"
     CDS             complement(28256..29800)
                     /gene="ilvA"
                     /locus_tag="ACHJ8C_001655"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO5551522.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29803..31653)
                     /gene="ilvD"
                     /locus_tag="ACHJ8C_001656"
     CDS             complement(29803..31653)
                     /gene="ilvD"
                     /locus_tag="ACHJ8C_001656"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO5551523.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQDDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDIIVTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRSNGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31718..32647)
                     /gene="ilvE"
                     /locus_tag="ACHJ8C_001657"
     CDS             complement(31718..32647)
                     /gene="ilvE"
                     /locus_tag="ACHJ8C_001657"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO5551524.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32667..32930)
                     /gene="ilvM"
                     /locus_tag="ACHJ8C_001658"
     CDS             complement(32667..32930)
                     /gene="ilvM"
                     /locus_tag="ACHJ8C_001658"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO5551525.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32927..34573)
                     /gene="ilvG"
                     /locus_tag="ACHJ8C_001659"
     CDS             complement(32927..34573)
                     /gene="ilvG"
                     /locus_tag="ACHJ8C_001659"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO5551526.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFSHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNIDDWQQHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCIVTTDVGQHQMWAAQHIVHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITHKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34576..34626)
                     /gene="ilvX"
                     /locus_tag="ACHJ8C_001660"
     CDS             complement(34576..34626)
                     /gene="ilvX"
                     /locus_tag="ACHJ8C_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO5551527.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34713..34811)
                     /gene="ilvL"
                     /locus_tag="ACHJ8C_001661"
     CDS             complement(34713..34811)
                     /gene="ilvL"
                     /locus_tag="ACHJ8C_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO5551528.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT         8456 a         9648 c         8832 g         7958 t
ORIGIN      
        1 tgtcagggag agggatctca attatcaatg cttaattacg tcatcatttt gacgacatga
       61 aaccggagca ttttgcatgg aattttcgtt ttcacccaaa cttcttgttg ttgctgtcgc
      121 cgcagctctt cctctcatag ccagcgcagc agataccccg tcaaccgcca ccgcacgcaa
      181 aggctttgcc ggatacgatc acccaaacca gtatctggtt aaaccggcga ccactattgc
      241 cgacaacatg atgccggtga tgcagcatcc tgcacaagac aaagaaaccc agcagaaact
      301 ggcggaactt gagaaaaaaa ccggtaagaa accgaacgtg gttgttttct tgctggatga
      361 tgtgggctgg atggacgttg gctttaacgg cggcggcgtg gcggtgggta accctacacc
      421 agatatcgac gccgttgcca gccaggggct gattttaact tcggcgtatt ctcaaccgag
      481 ttcttcccca acccgcgcca cgattctcac cggacaatac tccatccacc acggcattct
      541 gatgccgcca atgtacgggc aaccgggcgg tctgcaaggg ttaaccacgc tgccgcagtt
      601 gctgcacgat cagggctaca tcacccaggc tatcgggaaa tggcatatgg gggaaaacaa
      661 agagtcgcag ccgcagaacg ttggctttga tgatttccgc ggctttaact cggtgtccga
      721 tatgtatacc gaatggcgtg atgttcatgt caacccggaa gtcgccctga gcccggatcg
      781 ttctgaatac atccaaaaac tgccgttcag caaagatgac gttcatgccg tgcgcggcgg
      841 tgaacaggaa gccattgccg acattacgcc gaaatatatg gaagacctgg atcaacgctg
      901 gatggaatat ggcgttaagt tcctcgacaa gatggcgaag agcgataagc ctttcttcct
      961 ctactacggc actcgcggct gtcacttcga taactaccca aatgcgaaat atgcgggtag
     1021 ctctccggcg cgcacctctt acggcgactg catggtggag atgaacgata tcttcgccaa
     1081 tctgtataaa gcactggaga aaaacggtca gcttgataac acgctgattg tgttcacctc
     1141 tgataacggt ccggaagccg aagttccgcc gcacggtcgc acgccgttcc gcggtgcgaa
     1201 aggttctaca tgggaaggcg gcgttcgtgt gccaaccttc gtttactgga aaggcatgat
     1261 ccaaccgcgt aaatctgacg gcattgtcga tctggcggat ctcttcccta cggcactgga
     1321 tctggcgggg catcctgggg cgaaagtagc gaatttagta ccgaaaacca cctttatcga
     1381 tggtgtggac cagacatctt tcttcctggg aacaaatggt cagtctaacc gtaaggccga
     1441 gcactacttc ctcaacggta aactttctgc tgtgcgtatg gatgagttca agtatcacgt
     1501 cctgattcag caaccttacg cttataccca gagcggatat cagggtggat tcaccggcac
     1561 agtaatgcaa acggcgggat cgtcggtgtt taacctgtac accgatccgc aggaaagcga
     1621 ctctatcggc gtgcgccata tcccaatggg tgtaccgcta cagaccgaaa tgcatgcgta
     1681 tatggagatc ctgaaaaaat atccaccacg cgcgcagatt aaatctgatt aagccggcgc
     1741 tatcgctgag atctaccttt gccggatgcg atgctgacgc atccgacaat caaccgcagg
     1801 cggccacaaa atggctactt ttttacttat tcaccaccag caaatgcgcc tgcataatgt
     1861 cgctggccgg acgaccgtgc gccagcaaat cagccattgc tttaagatat ggcggtagat
     1921 ggcggaaata acgctgatac ccggcacaca aataattcag cccgggtttg ccgctggcat
     1981 cgagcataaa gcggtgtttc gggcagcctc cccagcacgc ttttaacacg ttgcaactgc
     2041 gacactgtgc cggtaactgc ttgaatttat cttcaccaaa tgcctgctgt tgcggggaat
     2101 caatcatttc tgcaatcgtc tgctgaagca tattccctag tcgatactgc ggataaacat
     2161 agtgatcgca ggcgtaaaca tcgccgttgt gttcaacaat caccgagcgc ccgcaggttg
     2221 gctgatgatg gcaaaccgcg cccggtgcgc cgacaaaatt ggcgaacgcc cattcgatat
     2281 tcattacgaa aatcttaccg acgtcgcgct taatccagtg gtcgaaaatc gccaccagaa
     2341 actcaccgaa ctcttgcggg cacaccgacc attccgtcag ttcgccctga atatcgccgg
     2401 gagcatgtag cttaagtcca gcatgagcag ctgtttcatc agccaggcgc tcgaccaccg
     2461 gaataaactg gatgaactca acacctgcat cacacagaaa atcatagact tgcaaaggtt
     2521 gtaacgcact ggtgcgattg acgcagacca gcacgttata gtcgacatga tgtttttgca
     2581 ggagcgtcag agcacgcatc accagcttat gcgttggtct gccacctttg gtcacgcgat
     2641 attgattgtg gatctcagcc ggaccatcca gcgataaccc tacaagaaaa tgattttctg
     2701 ccagaaatgc acaccattcg tcatcgagca gcacgccgtt agtctggaag ctattactta
     2761 tcttcctgcc agcaccatat ttcgcctgta gcgccacggc acggcggtaa aaatccagcc
     2821 cgagtaacgt tggttcgccg ccctgccagg taaaagccac ttcgttttgg gtttcgctgg
     2881 cagcgatata gtgacggaca tacgcttcca gcgtgtcatc gtccatatgc gtgactggct
     2941 tttcgcggta aagggattgt ttttcgagat aaaaacagta gtcacagttc agattacaat
     3001 cagaaccgct cggtttcgcc atcacatgaa aagcacgcgt tggaacctgt tgctgcatgg
     3061 tcgctccttg cctgaagtca tccttcaata agttaagggc gaaatcatac cgtggcgcaa
     3121 tgcgttatta cgggcgttta ttgcgaaggg aaagtgacga aatgcacaga aaacaaaaaa
     3181 ccccgccgaa gcggggttca aaattggtcg gcgagagagg attcgaacct ccgacccact
     3241 ggtcccaaac cagttgcgct accaagctgc gctactcgcc gaaatactgc tttttgaatt
     3301 tttagttcaa ttctttaaag tcgtggtgcg agggggggga cttgaacccc cacgtccgta
     3361 aggacactaa cacctgaagc tagcgcgtct accaattccg ccaccttcgc attgtcacaa
     3421 cttctaataa tggggtggct aatgggattc gaacccacga caactggaat cacaatccag
     3481 ggctctacca actgagctat agccaccact acaaatcttg ttacgcggta ttactaccac
     3541 cgcagctcaa gcgccgggac taaatggcgc gcccgacagg attcgaacct gagacctctg
     3601 cctccggagg gcagcgctct atccagctga gctacgggcg cttagcgccg ttgcgggggt
     3661 ggataatacg gacttcccac ccctctgtct agtccctttt taaataaatt gcgcgtttgg
     3721 ttacggtttg tgcgttttgc tgcttattcc tccagtttat gcgctttacc gtggcgatta
     3781 agcccaaaaa ctttataaat cgccgtcacc accagcataa agatgatgcc gacaaacagc
     3841 gacatacgcg tatcttcatt aaagtacatg ccgatcaaaa cgcaaatcag gaatgccatt
     3901 gttacgtagt tggcccaagg gaacaggatt gagcggaacg gatggctggc aattgccgct
     3961 ttgtgtgcac ggcgaaaacg cagttggctt atcaaaatca caaaccatgg caccataccc
     4021 ggaagcacgc tggcgctgta gacgtagaca aacacacgct gtggattggg aatgatgtag
     4081 ttcaggcatg aaccaattag cagaatagca atagatactg ccacacccgc aaccggtacg
     4141 ccgtgacggg aaactttcgc cattgccgcc ggtaactgac ggtttttcgc cagtgcatag
     4201 agcatacgtc cgcaactgta catgccgctg ttacagccag agagcgcagc cgtcagcacc
     4261 acaaagttga taatgcccgc cgctgcggta atgccgattt tggcgaaagt cagtacgaac
     4321 gggctaccgt tgctgcctat ttcattccac gggaagatag taacgataac gaaaatcgcg
     4381 ccaacgtaga aaatcaggat tcgccacagc accttgccta cggcactgcg cagcgtcacc
     4441 tgcggattct tcgcttcacc ggcagtaatg ccaatcagct ccacgccctg gtaggacgcc
     4501 accacaatac acaatgcggt caggaaccct ttccagccac cagcaaagaa accgccatgc
     4561 tctgtgagat tgctaaaacc aatcgactgc ccgccattgc caaagccaaa gaaaatcacg
     4621 cccaggccaa tgacaatcat cacgataatc gtggtgactt tgatcatcgc gaaccagaac
     4681 tcgatttcgc cgtacaacct caccgcagcc agattcgcca acgccaccag cgccaccgcg
     4741 atcaatgcgg gtatccactg cgccatctcc gggaaccaga actggacata aacgccaatg
     4801 gcggtgattt ctgagatccc caccgccatc cacataaacc agtaagacca ggcggtgaga
     4861 taaccaaaga acgggctcat ataacgatgc gcgtaaacgg cgaacgaacc ggtaaccggt
     4921 tcgaggaaca acatttcgcc cattgaacgc atgatgaaaa agacgaacag cccggcgatg
     4981 atataggcca acaatacgga tggcccggcc catttcaggg tactggcggc ccccataaac
     5041 aggccgacgc caatggtgcc ccccagggcg atgagttcga tatgtcgagc ttccagccca
     5101 cgctgtagct ctggtttgtt atctgccata aatcctcgtg ttgtgtttgc atgctttccg
     5161 gtgttaccgg ttatcgttat gggtacatcg agtgttgcga atgttttcgt aattcaggag
     5221 aaatggcaaa taaagcatta aaaatttgaa tgctttgtgt aataaaaaag cagacaggcg
     5281 acgaagtgac cactccgtcg ctttacaaag agaggaaaat cagaggttgc cggtgtaatg
     5341 ccagcgtaaa taacgcagca aacgaagctg acgcttaatg cggctcggct gcgaaagcag
     5401 gcggtagagc cactccagcc ccagcgtttg ccagattttc ggtgcgcgtt ttacgtgacc
     5461 ggtgaaaaca tcgtaggtcc cgccaacgcc catatacagc gcatctggat gcaccagacg
     5521 gcagtcgcgc atgaagatct cctgctttgg cgatcccatc gcaacggtga cgatttgcgc
     5581 tccgctggca tgaatgcgtt caaacagcgc ctgacgctgc tcgggtttaa aataaccatc
     5641 ctgactgcca acgatattca cattccactg gttgcgcagt ttagcttcag tttgcgccag
     5701 cacttcaggt ttaccgccca caagaaatac cggtgtccct tctttgcctg cgcgcgccat
     5761 cagctcttcc cagagatcgg ccccagcaac gcgggaaacc tgcgcctgcg ggtacttttt
     5821 acgtactgaa cgtacaacgc tgatgccatc cgcatattta aattcggcag cgttaattaa
     5881 ctccctgacc tcggcgttat cttcaatggt cagcattttt tcggcattaa tggcaaccag
     5941 cgttccctgc ttaagctgcc cgtcagcgaa cagataatcg agggcatgct gcatatcacg
     6001 ccaaccaatc aactgtaagc cacgcagcgt ataggtcggt gccgtggtgt tgttattcat
     6061 tgttatcctt caacctgcgt ccggagcgat gattttgttc gtttatgaat gagtccggcg
     6121 ctttcaaaaa gccagtacaa cagttttgcg atcatcagac atgcgccgaa gaccacgata
     6181 aaaaagacca cgcgtgagac aaacgaatcc agcccttcac gcgccagcac gatcatattg
     6241 aagatcgccc cgaagcagaa actgtgcaat atcgcagcct tatagcgatt agtctcgcgg
     6301 ttgcccagct catacagcca gtcgaaccat ttgataatca gtccaaccac aatcgccccg
     6361 agcgggatga acaacgcgcc gcccataaca accagtgagc ctataagcgt aggcgagatc
     6421 gccagtccgg agtggttatt cagcacttcc caggtaaagt agttggcgga gttcagcacc
     6481 atactcgggc gacccggcca cagccaggaa gggataaaga catagaaatc gcggacaatt
     6541 ggagccaggc cctggaagtc gatgttgtcg tagttctgca acagcaacgc cagattctcc
     6601 cacggcgaga aagtgtcgcg agtgagatag agaaacgtat agaacgcttc atcgccgctc
     6661 acattcattc catagcgttt tagcgccagc cagaacatgc ccacaatccc cagcacgccc
     6721 gccgccgcca gcatccacaa cgaaatccag ccgcgaataa tgccaataaa caggaagata
     6781 gcgaatgcga tgatgatatt ggcgcgagtg ccgccgacaa tcatgtaagt caacaagcca
     6841 aaggcaaccg tgctgacgag gaaaaacagc cacgctttgc tatcctggcg cagaaaataa
     6901 accaccagca tcgccgggat gaaaaagtaa aagaagcgtt ttaatgccac gccggagact
     6961 tcgctggaaa agatctgact gtaagaatta agccggaaca gcaaaaagcc gttgtgcatg
     7021 aagaagatgc cgacgctcac cagagcgatg cccatcagga tcacccacgt gagattggtc
     7081 tccacgcggt tcatggtaaa cagcggacgg cgcggtgcat cagcaacgcg tttgcgtaga
     7141 cgggttttgt aggtgacata gtaaaccgcg tagaagcagc ctgcagaaag caacgcctgc
     7201 aacaagattt ctggcggcgc gacaccaaca tcaaagcgaa ataccagcac gctggtcagc
     7261 ggaaagccga agaaaaaggt gagcaaaaac agcaatgaaa agaagacatt gaagttaaag
     7321 cgcacacggc gaaactcaaa ccaggtcagc gtggcaataa acagcgtgca gagcagccag
     7381 acaacaaaca ggccactgaa ttgcagcaga ctcatgcgac ctccccggcg gcaatcgcca
     7441 acgcccgctg ccagccttgt agatagttag ggctaaagaa ggcaatggtg tttttatcca
     7501 ccgacgccaa ctggcgctgc gcttcacgca caatatcctc gttgagatcg tcggtagtaa
     7561 acagcactgg caaatgctgt tccgtcatat cctgccagaa cggattttcc cggttaagca
     7621 cacaaggaat gcccgcctga atcagtaagc acagcgtacc aatgccctgc tggcgggcaa
     7681 aaataaagta accaagatcg cactgacgaa gtagcgtcag ataggcgtca aattccagtt
     7741 tttcgctcag aacttgtagg ttttcttcgc tgaataactc cagtcccgcc tgacgaactt
     7801 cctcgatgta cgcttcgtta ttaggcggat atcccatcgg caccaccact tttaccgtat
     7861 cgccaaattg ctgatgaacg gcgcgcaagg cagcaatatg ctcattgctg cggtcaccgg
     7921 agttccccac cagaatggtc atttttcctt cacgttgccg atcgttcgcc atcgtattaa
     7981 gagaagggtc catccgcgtc gggaagtaca gcagttctcc ccgcaccttt ggatgcgttt
     8041 tggcaaaaaa gctcaaatca ccgcgggtgg caaatacaca gccgactctc ttttgcgcca
     8101 ggcgacgtag cgggtaaaaa agcttatatc tcaagccact ggaaagctca tacaggtctg
     8161 ctccccagat atgccagaaa aactggctgg gcttaatgcc accactcagc agagccagcc
     8221 acagtttggg attgaactga ccgtggaaaa aaaatcgctg ctgacggtta gcttttgctt
     8281 tcgcgatgac cgcttccgcc agcgattttt tcccagggaa aaattgcaca gaaagcgccg
     8341 gacagctatc acttaagccg tcgtccttgc cagcaaccat aaactcgcgc gcgtgcccgc
     8401 tcgtcgcggc cagcgcgtcg ttgaaaaacc gcagaacggt tcggttatgg tgagggatat
     8461 ccgatcccag tacgtgaatc agtacagtca tgcccgccta cgccagagta aaaacacgcc
     8521 acaacaaaga gaaaaataga cgatataggt tgccatatag gcctgtgctg cgcccagtgc
     8581 gccatgcgca gggattagcc agtgggcaaa taccatcaat aaagtgaact ggctgatttc
     8641 cgccagaata taaaaccgca gtgacgcttt ggcgatcacc agataaccaa agacataagc
     8701 gcccactttt aacacatcac ccaccaactg ccaggcaaag agatcgcgca tagcggtaaa
     8761 tttattcgac aacagcagcc agatagcaaa atcacgcagc aaccagacgg taaaactcgc
     8821 cgccgccact gccggtaaga cgaatttcag cgatttaacc acttcccggg tgatatcgcg
     8881 cttttccgtt aaccgcgaca acgtgggcag caaatatacg ctgaacgatg ccgtaataaa
     8941 ttgcaggtag gcatcggaaa tactgctcac cccttgccag atccccactt catcccagct
     9001 atactgcgcc gccagcagtt tacgcatcat gatgtaagca acaggcaagg tcaccgacgt
     9061 aatcaacgcc atgagcgtaa atttgctcaa ctgccctgcc agaccgttat cccagctggg
     9121 tttcagatag cttaacggga tgacaccacg tttgatcaac atgataacag caggaattac
     9181 caccagcgcg ggaatcagcg ccagacccag caacgcccct tcataaccgc ccaaacgata
     9241 actgacgtag tacgcgagaa cgccaatcaa gctgccgaca atcagcgata acgcattacc
     9301 tgcggcatcg cgaaagcctt tcatcaacgc cagtaacagg ttgccccagg caatccccat
     9361 ttgcaccagc gccactaaac gcaccagccc ctgatagtcg gtattaccaa acaacccctg
     9421 gctgattggc gcagctgcca gcacaaaaac cagcgccatt agcgtagaga agccaagtac
     9481 catcgctgat gaagtgccga ccacgcggcg cagctgttgg ggattatcat gatactgggc
     9541 aacgtatttg gttacgccgt taaagatgcc tgccccggca agcacgccga gcacggtaat
     9601 caactggcgg aaatttgccg ccagcccaag ccccgccgga ccaaatgaca ctgccagcaa
     9661 cttaccgacc agtaacccgg caccaatctt gaccagtgta ctggccgcag tccacaagga
     9721 cgcttttgcc aacgacatat caggagaagt agttcaacaa ggtcgcaatt accgtacgct
     9781 gattgacggg cgacaggttg tagaacaacg gcaggcgcag caggcgctcg ctctctttgg
     9841 tggtgtagcg atcttcaccg tggaactcac caaagcgttc ccccgcaggg cttgcgtgca
     9901 gcggaatgta atggaacacc gccatgattt ccgcttcctt caggaagcta atcaacgcgc
     9961 tccggtcatc aatatcccgc agcttgatgt agaacatatg cgcgttctgc acgcagtcat
    10021 ccggaatcga cggcagctcg atacgcccgg cttccgccag aggtgctaac gcatcgtagt
    10081 agttttgcca cagcgccaga cgttgctggt tgatgcgttc ggctgcttcc agttgcgccc
    10141 acaggtacgc agcttgcaga tcggacatca aatagctgga gccaatatcg cgccaggtat
    10201 atttatcgac ctgaccacgg aagaactggc tgcggtttgt gcctttttca cggatgatct
    10261 cggctcgttc gatcaacgct ttatcgttaa tcagcgtcgc gccgccttca ccacccgccg
    10321 tgtagttttt ggtttcatgg aagctaaagc agccaatatg accaatggtt cccagcgcac
    10381 gccctttgta agtggacatc acgccctgag cggcatcttc caccacaaac agattatgct
    10441 tcttcgccaa cgccataatg gtgtccattt cgcaggccac acccgcgtaa tggaccggca
    10501 cgataacgcg cgttttgtcg gtgatcgccg cttcaatcag cgtttcgtcg atgttcatgg
    10561 tgtccgggcg aacatccaca aagacgattt tcgcgccacg cagcacaaag gcattggcgg
    10621 tggagacaaa ggtgtagctc ggcatgatca cttcatcgcc aggctggata tcgagcagca
    10681 gcgccgccat ctccagcgaa gcggtacagg atggcgtcag taacactttg gcgctgccaa
    10741 aacgttgctc cagccactgc tggcagcgac gggtaaaacc accatcgccg cacagtttgc
    10801 cgctacccat tgccgactgc atatagtcga gttcggttcc caccaccggc ggtgcgttaa
    10861 atggaatcat gtgatcacct gtataaccag tacgcggtgc tttctacatt cgcaccactt
    10921 tgtatgtatc gtttaagcgc ggcggtgttg cccatctggg tcgccacccg caaagttgtt
    10981 ttaccgcgag catacgccca gtttagcgcc gtttgcatta gctcagcacc tgcaccgagt
    11041 ccagccagca ggccaattcg cgcatctgtc gcattgagtt cccgtaaaga gacataaccg
    11101 cgaatatcgc cggacgccgc acgtaaaatc agacattgat gatcaaaggt gccgcgcacg
    11161 gcattttcaa tccactgtgc ataaaagcga ccgctggcgt caggcgcata ccacggcgca
    11221 cgaaaacggc tttgcgcaaa tgcggcgctg gctaactgac gtaatgcggg aatatcggtc
    11281 tcttgtgcca ctactgcacc actatcactg acattgttca cgggtagcgc caaatcaact
    11341 tcaccttcta ccagggagaa tcccagctgt tgcagggcat ccagttcccc cgtatttgat
    11401 gccgcaattt tggcctgcac ccgtgaccac ggcgctaacg cgtctggcgt caggagcggt
    11461 gcttcagacg taatgcgcac gatggcgctg ttaacaccaa agaaggcgtt ttcccaggtt
    11521 agtggctcaa tactggcgcg gacgggcacg aagtaactcc agcagatatt ggccgtagcc
    11581 agttttcgct aatgaactgg cagcacgctt cacaccctcg tcatcgagcc agccgttacg
    11641 ccaggcaatc tcttccaggc aggcaatctt aaagccctgg cgtttttcta ctgtctgcac
    11701 aaaagtgctg gcctcaatca ggctgtcgtg agtgccggta tccagccagg caaatccgcg
    11761 cccgagcagt tcaacggtca ggttgcccgc ctcgaggtac atctggttga tggaggtaat
    11821 ctccagttca ccacgctccg acggcttcac ctgctttgcg tactccacga ctttactgtc
    11881 gtagaaataa agcccggtca ccgcccagtt tgatttcggc tgttttggtt tttcttccag
    11941 cgagatagcg cggaaattgt cgtcaaactc caccacgcca aagcgttccg ggtccatcac
    12001 ctgatagcca aataccgtcg ccccttccgt gcgcgccgca acatgacgca gcttcggact
    12061 gaagccctgt ccgaagaaga tgttatcgcc cagcaccaga caagaaggtt caccgttgag
    12121 gaaggtttca ccgatgataa aggcctgcgc cagaccgtcc ggactgggct gttcggcgta
    12181 ttccagctgg ataccgaact cactaccatc gcccagcagg cgctggaaat aacctttatc
    12241 ttccggcgta gtgatgatga gaatttcgcg gataccggcc aacatcagca ccgacagcgg
    12301 atagtaaatc attggcttat cgtaaatcgg caacagttgc ttcgatacgc cgcgcgtaat
    12361 cggatgcaat cgggtgccgg aaccgcccgc caggataata cctttcatgt ttgcctccgc
    12421 tgaaaattag cctttcagac ctaaacgctc gccctgatag ctgccgtcct gcacctgctt
    12481 ccaccagatt tcattagcca gataccactg caccgtttta cgcattccac tttcaaaggt
    12541 ttcctgcggc agccagccaa gttcacgggc aattttcgaa gcatcaatgg cataacgcag
    12601 atcatgcccc ggacggtcag cgacaaaggt gatcaggtca cgatagtgcg ccacgccgtg
    12661 cggcttattc ggagccagtt cttccagcag ctcgcaaatg gtttccacaa catcgagatt
    12721 cttacgctcg ttgtgaccac caatattata ggtttcaccg actttcccgg tggtcgccac
    12781 gcaatacagc gcgcgggcgt gatcttcaac atacagccag tcacggattt gctgcccgtt
    12841 gccatatacc ggcagcggtt tacccgccag cgcgttgagg atcatcagcg ggatcagttt
    12901 ttccggaaag tggtaaggac cgtagttatt cgagcagttg gtgataagcg tcggcagacc
    12961 ataggtccgc agccaggcgc gcaccagatg gtcgctgctg gcttttgacg cggaataagg
    13021 gctgctcggc gcatacggcg tggtttcggt gaagaaatca tccgtcgagt gcaggtcacc
    13081 atatacttcg tcggtggaga tatgatgaaa acggaacgct gattttttat cttccgtcag
    13141 cgcagtccag taagcccgcg ccgcttcaag cagtgtataa gtcccgacaa tgttggtttc
    13201 aataaacgct gccgggccgt caatagaacg gtcaacatgg ctttcggctg ccagatgcat
    13261 gacacagtct ggctgatact cagtgaatac gcgcgccagt tctgcccgat cgcagatatc
    13321 aactttctca aaggcaaagc gctcgctttg cgcgaccggt gccagagaca tcaggtttcc
    13381 ggcgtaggtc agcttatcga ccaccaccac cgcgtcgctc gtttcgttga tgatataacg
    13441 caccagcgcc gagccaataa acccggcacc acctgttatc agaatctttc tcatcagcgc
    13501 cagactcctt tggcatcgac gacatactgc tgatggacgt tgtcgccatt gataactttg
    13561 aactgactat gatcgaccag catcaccagc acatctgccg ttgccagcgc ctcgtcaagc
    13621 tgcgccagag tacaaagccc ggtcagtttc ttcggcaact ggtggatgtt aggctcaaca
    13681 accagcgttt cgccgctgtg ccactgggcg atcagttcag cgatttccat cgcagggctt
    13741 tcacgcaggt catcaatatt cggtttaaac gccagaccaa agcaggcgat tttcagttca
    13801 ctggcgcgtt tatcggtagc agccagacaa tcagccaccg ccgctttcac ctgatcgata
    13861 acccagaacg gtttgtgatc gttcacttcg cgcgcggtac ggataagccg cgcctgctgg
    13921 gggttctgtg ccacgataaa ccacggatca acagcgatgc agtgaccgcc cacgccaggg
    13981 cctggctgaa gaatattgac gcgagggtga cgattcgcca ggcgaatcag ttcccagacg
    14041 ttaatcccct gatcggcaca aatcagcgac aattcattag caaaagcgat attcacatcg
    14101 cggaagctgt tttcggtgag cttacacatt tccgccgtcc gcgagttagt gacgacacac
    14161 tcaccttcga ggaaaatttt gtacagttcg ctggcgcggg ccgaacaaac cggcgtcata
    14221 ccaccaatca cgcgatcgtt tttaatcagc tcgaccataa cctggcccgg taatacgcgt
    14281 tccgggcagt aagcaatgtt gacgtccgcc aactcgccca cctgctgtgg gaaagtgaga
    14341 tctggacgca tctctgctaa ccattctgcc atcttctcgg ttgaccccac cggcgaggtg
    14401 gattcaagga tcactaacgc gccttttttc agcactggcg caatggagcg agcagccgat
    14461 tcaacgtagg tcatatctgg ctcatgatcg cctttaaacg gtgtgggtac agcaatcaac
    14521 caggcatccg cttcaactgg cgtcgtgctc gctcgtaaaa aaccgccttc tacggcagtt
    14581 tttactacgc tcgccaaatc aggctcgacg atatggattt cgccacgatt gatggtatca
    14641 accgcgtgtt ggttgatatc gacaccaatc acctgttttt gccttgaggc aaacgcagct
    14701 gctgtaggca gcccgatgta acccagtccg ataacagaaa tggtcgcaaa actcatagtg
    14761 atatccgatt attttttaac gcttccagaa tgcgagagca tgcctgacca tcaccatacg
    14821 ggttatgggc gcggctcata gcttgatatt cgttttcgtc ttttaaaaga cgcgtcactt
    14881 cctcgacaat tcgctgctta tccgtgccga ccagacgcac cgtccctgct gtcaccgctt
    14941 ccggacgctc agtggtatcg cgcatcacca gcaccggttt ccccagcgaa ggcgcttctt
    15001 cctgaatgcc gcctgagtca gtcaaaatca gccaggcgtg gttcatcagc cagacaaacg
    15061 gtaaatactc ctggggatcg atcagaatga catttttcac atgccccaga atgcgattga
    15121 ccggctctct gacgttcgga ttgagatgca ccggatagac aatctggatg tcctggtgcg
    15181 tggtggcgat gtctgccagc gcatggcaga tttcttcaaa gccacgaccg aagctctcac
    15241 gcctgtgacc ggtcaccaga atcatctttt tatcggggtc gataaacggg taatttgccg
    15301 ccagttctga acgcagcgta tcgctgctca tcacctggtc acgcacccat aacagtgcat
    15361 caatgactgt attaccagta atgaagattc ggctatccgc aacgttttca cgcagcaagt
    15421 tttgccggga agtttcggtt ggagaaaaat gatacatcgc cagatgcccg gtcaatgtac
    15481 ggttagcctc ttccggccac ggcgaataga gatcgcccgt gcgcagacca gcctcaacgt
    15541 gaccaacagg aatacgctga taaaacgccg ccaggctggt tgccagcgtc gtcgtagtat
    15601 cgccgtgaac cagcacgaca tctggtttga actcggcaag aataggtttt agcccttcca
    15661 gaatccgaca ggttatctct gtaaggccct gtcctggctg cattatgttg agatcgtagt
    15721 caggtacaat ggaaaagagt ttcagcacct gatcgagcat ctcccgatgc tgcgcagtga
    15781 cgcaaacttt agcctcaaaa aaaggatctt ttgccaacgc atgcaccaac ggtgccatct
    15841 tgatggcttc cgggcgcgta ccaaatacag tcagtacttt cacatcgatt ctcttcgaat
    15901 aagcggcgag cgcctttgcg ctcaccgcag cagtgttgct atttcgagca acggcgggtt
    15961 aatgcgacac cagccccgat cagtcccccg acaatgcccc acataatcat caggaaggca
    16021 cgacgtgggc tatcgcgttt taccggttct tccggcgtac gcaaatagcg ataggtctga
    16081 aaacgcggat ccagggttgg accaacattc agggtgttta acatggcccg attctgatca
    16141 tagtcgagat caaaggccgg accgacggcc tgtaaatttt ccagtcgagc ctggagcatt
    16201 ggacgcccaa gcaggaacat ttctgaatca ggtaattcct cggcaggcac atctgtcgca
    16261 ctgcgcgaaa tattatgctg ctcagcaatt ttcagcgcct gttcaatact gttcatccgg
    16321 cgatcgtaga tggctttcgc cacctcttcc tgacgcttca cctgagcttt catctggatg
    16381 gtgcgtgccg cccatgcgcc tttcagctca tcattcagat ggctggctgc acgctggctg
    16441 gcaaaagcaa catactgacg taacaggtta ttagcgtcag gcgcagtttc ggcaatcagc
    16501 ttcacgctgt cattgaccgc gcgggtgaag tcaccgggaa taaacacgat gttgttgatc
    16561 atttcatcca gcaacgccgc atcggctttg ctattgccca ccatccgctg tttgtaatag
    16621 tcggtttgca gccagaactc tctgcgggta tcccacgagg ccagttgcat aacaaactct
    16681 ttataggctt cgtccatgac cgatggttgg tcggcagaag ccatgtttga acggacatcc
    16741 aggttacgca aaaattgctg ctgtgagtaa tatcccccca gcatattcac cgttggacga
    16801 tcggtaatcg ctgtcgagct ccactcctgg cgagcaaaaa aagtatacgc cagcgcgatt
    16861 aacgcaaacg ccagccccat gccaataatc cacagcttcc cagcccacaa ggtacgaaac
    16921 aacccacgaa tatccagttc attttcagcg tcttcggccg gtttcccagg cattggttgt
    16981 gtcatcacat cctcatttat ttggttaaat tggggctgcc accacgattt ctacgcagtc
    17041 tgcgttttac gcgcttaata aagcgagcaa ctttccaggc acgcttgatg caatatccat
    17101 agaggaagaa tgctagcaaa aagagcacca gcatgaccca ctccgggaca aaatgagaat
    17161 attctgccag cacgccaatg gaagcgagca gtgctgcggc aagggtaatc agcacaaacg
    17221 cctggcggga agtaaacccg gcacgcatga tcaaatggtg aatatgctga cggtcaggag
    17281 agaatgggct catgccttta cgcaggcgac ggtacataat cgccaccata tccattagcg
    17341 gaatggcgat aatccacaaa gcggtaaccg ggctgatggg atgagttttg ccctgggtcg
    17401 tttcgagcag gatccagatc acggtaaaac caatcagcgt actgcccgca tcccccataa
    17461 agactttgta gcggcgaccc aggataccaa ggttaagcat gatgtatggc aggatggcgg
    17521 cgatcatcgc aaagcaccag attgcgagac tggtttgccc gtcgaaccac aaaatcatac
    17581 cgattgctgc aaacgagacg caggacaacc cgcccagcaa gccatcaatg ccatcaacca
    17641 tgttgaacgc attaatggcc gcccagacgg caaatagcgt caggaagtag ccaaacggtc
    17701 cgagcaccat ctcccaggag ccaaagatat aacccaggct actgagataa agttttccga
    17761 acaccatcat aacaatgcca acagcggcct gtatggtggc acggattttt acgctgatat
    17821 caaaacggtc atccagcgcg ccaatgaaaa caagcacacc ggcacaagcg agatagagag
    17881 atgcatgcgg aatatagtaa tcgacaattc cgaacgtgaa gcaaatccct gcgtaaaccg
    17941 aaatcccccc aacgagaggt atcaatccct ggtgacgttt gcggaagtta ggtttatcca
    18001 ctaaaccgac tttttttgcc accttacggg caaaaaacag aaacagtgtc gtgaataaaa
    18061 aaatactgat gagatcagta ctcactgtca gtaaattcac aatgcatgct ctcagagaaa
    18121 attattagca gaagtataac cacgaagacc tttattcaga agggaaagtc cgttccgaac
    18181 ccaccagccc tgtacaaaaa ttaaccattt gatatgcaaa ttgcataaca caccagcaaa
    18241 atccaggaat tttcctaaca ttgccagcgc ggcattaaga ttacagatat aaaagcaaaa
    18301 cgccacgtaa acacgtggcg tttttggcat aagacaaatt tatgagcgtt tcatcatttc
    18361 gaagaaatcg tcattggtct tggtcattgc cagtttatta atgaggaatt ccattgcatc
    18421 gatttcgccc atcgggtgaa tgattttgcg caggatccac attttctgca gttcttcctg
    18481 agtcgtgagc agctcttctt tacgggtacc ggaacggttg tagtcgatag ccgggaagac
    18541 gcgtttttca gcgatcttac gagagaggtg cagttccatg ttgcctgtac ctttaaactc
    18601 ttcgtagata acttcgtcca ttttagaacc ggtatcgata agcgccgtcg cgataatggt
    18661 caggctgccg ccctcttcca cgttacgcgc cgcaccgaag aagcgtttcg gacgatgcag
    18721 ggcgttggcg tccacaccac cggtcaacac tttacctgac gccggaacaa cggtgttgta
    18781 agcgcgcgcc agacgagtga tggagtcgag cagaatgata acgtctttct tgtgctcaac
    18841 caggcgtttc gccttctcga tcaccatttc cgcaacctga acgtggcgag atgcgggttc
    18901 gtcaaaggta gaagcaacaa cttcaccttt taccagacgc tgcatctcgg ttacttcttc
    18961 cggacgttcg tcgatcagca gaaccatcag cacacaatcc gggtggttgt aagcaatgct
    19021 ctgagcaatg ttctgcagca gcatggtttt accggctttc ggcggtgcca caatcagacc
    19081 acgctgacca cgaccgatag gtgatgccag atccagtacg cgagcggtta aatcttccgt
    19141 agaaccgtta ccacgttcca tacgcagacg agagtttgcg tgcagcgggg ttaagttctc
    19201 aaagaggatt ttgttgcggg cgttttcagg tttgtcgaag ttaacttcgt taactttcag
    19261 cagcgcaaaa tagcgttcac cttctttcgg cgggcgaatc ttaccagaga tggtatcacc
    19321 agtgcggagg ttgaaacggc ggatttggct aggggaaacg tagatgtcat caggaccggc
    19381 gaggtaggag ctgtctgcgg aacggaggaa accaaatcca tcctgcaata tctccagtac
    19441 gccatcacca aagatatctt cgccactctt tgcgtgctgc ttcaggatgg caaaaataat
    19501 gtcctgctta cgcatacgag ccaggttttc cagccccata ttttcgccga gagtgatcag
    19561 ctcagaaacc ggcgtattct ttaattcggt aagattcata gtggtgtgag ttcttaaact
    19621 tggggtaaat ctcgaactta atgttgtgaa tggtatggca ggatcatcca tgcctgttta
    19681 cggtcatcga ctcatgtctt ttcgctgcct ggtcacagga gagtacgcag aactgaaacg
    19741 acaagacgga ttgagtgacg agccagaaat ctgttcactt cgcatttaag ataaaacggg
    19801 aagcgttgga aattacaaga ttcaaactta ataaggtatg tttaatacga agtcaacact
    19861 aagttagcat gactcacgcc gggcgtccag tttttagcga cggggcaccc gaacatgaaa
    19921 ttcccttacg ccaggttagc gtcgaggaac tctttcaact gacctttaga cagtgcgccc
    19981 actttggttg ccgccacttc accgtttttg aacagcagca gagtcgggat accacggatg
    20041 ccatatttcg gcgcagtgcc agggttttga tcgatgttca gttttgcaac ggtcagtttg
    20101 ccctgatatt cgtcagcgat ttcatccaga atcggggcga tcattttgca cggaccgcac
    20161 cactctgccc agaaatcgac gaggatcgcc ccgtccgctt tgagtacatc cgtgtcaaaa
    20221 ctgtcgtcag tcaggtgaat aattttatcg ctcatatata actccacagg aataagcctg
    20281 gcgtgttggt ttcgttgttg gtgtaacatt aaccaactaa aggttgactt tatttcaccg
    20341 gatacgcttt cgtaaagcaa tagtaagctg atattctacc acactatgag caaaacacat
    20401 ttaacagaac agaagttttc cgacttcgcc ctgcatccga aggttgtaga agcccttgaa
    20461 aaaaaagggt ttcataactg tacgcccatt caggcactgg cccttccgct gacgctggcg
    20521 ggtcgtgacg tagccgggca ggcgcaaacc ggtaccggga aaacgatggc gtttcttacg
    20581 tcaacgtttc attatcttct ctctcatccc gcgattgccg atcgcaaggt gaatcagccg
    20641 cgtgccttaa ttatggcacc gacgcgtgaa cttgccgtgc agatccacgc cgacgcggaa
    20701 ccgctggcgg aagctaccgg cctgaagctg ggtctggctt acggtggtga tggctacgac
    20761 aaacagctga aagtgctgga aagcggcgtt gacattctga ttggcaccac gggtcgttta
    20821 attgactacg ccaagcagaa ccacattaac ctcggtgcca ttcaggtggt ggtactggac
    20881 gaagccgatc gcatgtacga tctgggcttt attaaagata tccgctggct gttccgccgt
    20941 atgccgcctg caaaccagcg cctcaacatg ctgttctccg ccacgctttc gtaccgggta
    21001 cgtgaactgg cgttcgagca gatgaacaat gccgaatata ttgaagtgga accggaacag
    21061 aaaacgggcc accgtattaa agaagagctt ttctaccctt ctaacgaaga aaaaatgcgt
    21121 ttgctgcaaa cgctgatcga agaagagtgg ccagacagag cgattatttt cgccaacacc
    21181 aaacaccgtt gtgaagagat ctggggccac ctggcggcag atggtcatcg tgtcggttta
    21241 ttgacaggcg atgtcgcgca gaaaaaacgt ctgcgtattc ttgatgaatt tacccgtggg
    21301 gatctggata ttctggttgc caccgacgtt gccgcgcgtg gtttgcatat tcctgctgtg
    21361 acacacgtct ttaactacga tttacccgat gactgtgaag attacgttca ccgtattggt
    21421 cgaacaggtc gcgcaggcgc aagcggtcac tctattagcc tggcgtgtga agagtatgca
    21481 ttgaatttgc ctgctattga aacctatatt ggtcactcaa ttccggtaag taaatacaat
    21541 ccggacgcat tgatgccaaa accgctgcgc ctcacgcgcc cgcgcacagg caatggtccg
    21601 cgtcgtactg gcgctccgcg taatcgtcgt cgttcaggtt aaagaaaaaa tgtcatactc
    21661 caaatcattc aagctgtatc aagatggcaa gagaatgaat cccgatgcgc ttactgtcgt
    21721 aagtgattca ggtgaaagaa tgcagccaac acagagacag attgaaggat gaagagtatg
    21781 ggttccacct cgtcgctgta tgcagccatt gatctcggtt cgaatagttt tcatatgctg
    21841 gttgtgcgcg aggtggctgg aagcatccag acgctgacgc gaataaaacg caaagtgcgt
    21901 ctggctgctg gcctgaatag cgaaaatgcc ctgtctaatg aagcaatgga gcgcggttgg
    21961 caatgtctgc gcctgtttgc tgaacgtctg caagatatcc ctccctcgca aattcgcgtt
    22021 gtcgctacgg cgacattacg ccttgccgtc aatgcggggg attttattgc caaagcgcag
    22081 gaaatcctcg gttgtccggt acaggtgatc agcggtgaag aggaagcgcg tctgatttat
    22141 cagggcgttg ctcacaccac tggcggtgcc gatcagcgcc tggtggtgga tatcggcgga
    22201 gccagtactg aactggtaac cggcacgggt gcacaaacta cctctttgtt cagcctgtcg
    22261 atgggctgcg ttacctggct ggaacgctat tttgccgatc gtaatctggg gcaggaaaat
    22321 tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc cggttgccga tgaattacgg
    22381 tatcacggct ggaaagtgtg cgttggcgct tccggcaccg tgcaggcgtt acaggaaatc
    22441 atgatggcac aggggatgga tgaacgcatt accctggaaa agttgcagca actgaaacaa
    22501 cgcgccattc attgcggtcg gctggaagag ctagagattg acgggctgac gctggaacgt
    22561 gcgttagtgt tcccgagtgg tctggcaatc ctgatagcca tttttaccga actgaatatt
    22621 cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc tggtctacgg tatgttgcat
    22681 cttaccgtcg agcaggatat tcgcagccgt acgctgcgta atattcagcg ccgctttatg
    22741 atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg ctaacttctt cgatcaggtg
    22801 gaaaatgaat ggcatcttga agcaataagc cgcgatttgc tcatcagcgc ctgtcagctt
    22861 catgaaatcg gcctgagcgt tgacttcaaa caagcgccgc aacacgctgc ttatctggta
    22921 cgtaatctgg atcttcccgg ttttaccccc gcacagaaaa aactgctggc gacgctactg
    22981 ctcaaccaga ctaatccggt cgatctctca tcgctgcatc agcaaaatgc cgtaccaccg
    23041 cgcgtcgcag aacaactctg ccgtttactc cgcctggcta tcatttttgc cagccgtcgc
    23101 cgtgacgatc tcgtgccaga gatgacatta caggctaacc atgaactgtt gaccttgacg
    23161 cttccgcaag gttggctaac ccaacatccg ctgggtaaag agattattga tcaggaaagc
    23221 cagtggcaga gctatgtcca ctggccgctg gaagtgcatt aatgagtaag tgccggatgc
    23281 gatgctgaca catcttttcc ggccttgatt atttccctcg ttttgccgcc atcatcgctt
    23341 tcagattcgc cagatggctt tgccctttct gcatccgttc ttcggcgctg accactttgc
    23401 gctcctgttc ccaaatcaaa tcatcctgcg gcagctccag caggaagcgg ctcggctccg
    23461 ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag cgtaaaggtc aattccttct
    23521 gggcgcgggt aatgccgaca taggccagcc gccgctcctc atcgatatta tcttcatcga
    23581 tactgctctg gtggggcaaa aatccttctt ccataccaac catgtagaca tagggaaact
    23641 ccagtccttt tgacgcgtgc aaagtcatca gttgcacctg atccagctct tcctcactct
    23701 cgccgcgctc catcatgtcg cgcaaagtaa agcgcgtcac cacctgggtg agcgtcatcg
    23761 gctcatccag ttcgctgcct tcgagcatct ccgtcatcca gctaaacagt tggttgacgt
    23821 tcttcatgcg catttcggca gctttcggac tgggcgatgt ttcgtacagc caggattcat
    23881 aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg ctcccgctcc gccagacgct
    23941 ggatttctgc cagccagtga gtgaagcggg tcaatgcttc ataaccacgt ccgctaagcg
    24001 tctgactcag gcccatatca aagctggcgg taaacatgct tttattgcgc gtcatcgccc
    24061 attcacctag ctttttcagc gtcgccgggc caatctctcg cttcggcgtg ttaacgatac
    24121 gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg cagataagcc agcaagtcct
    24181 tgatttcagg acgagagaaa aacgacgtac cgccagatat tttgtacggg atgcggtttt
    24241 gcatcaggaa tttttcaaat acccgcgact gatggttgcc gcgataaaga atggcgtaat
    24301 ctttgtactg cgttttattg acgaagtgat gcgcgatcag ctcgccggta actctctcgg
    24361 cttcatgttc ttcgttattc gcgcttaaaa cttttagctc cgcgccatag cccagttcgg
    24421 agaacagacg cttttcaaag acgtgcgggt tattggcgat caggatgttc gccgctttca
    24481 gaatacgtcc ggaagagcgg tagttctgct caagcttaat caccttcagt gccgggaaat
    24541 cctgactcaa cagcaccagg ttttgcggac gagcaccgcg ccaggagtag atcgactgat
    24601 cgtcatcgcc aactacggta aagcgcgcgc ggctgcccac cagcagtttc accagttcat
    24661 actggctggt gttggtgtcc tgatattcgt ccaccagcag gtagcgaatt ttgttctgcc
    24721 agcgctcgcg gacttcttca ttgcgttgca acagtaacgt cggcaataaa atcaggtcat
    24781 cgaagtcgag aacgttacag gctttcaggt gtgcatcata cagcccataa caatgggcaa
    24841 aaatacgatc ccgctcgccc tttgcctctg ccgccgcctg cgccggagtt ttgagatcgt
    24901 tcttccagtt ggagatggtc gaaatcagct gttgcaggag aactttgtca tcttcaatca
    24961 gcccctcggt caactcttta agcaaagcaa gctgatcggt atcgtcaaac agcgaaaagt
    25021 tagccttcat ccccagcgcc gcatactcgc gtttgatgat atccagcccc agcgtgtgga
    25081 aggtggagat catcagccca cgcgcctctt tgcgccccag cgtctgcccg acacgctctt
    25141 tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc gatatgtcgc gcctgataac
    25201 cgcaaccgcg gatcaggtgg gcgattttat tggtgataac acgagtttta ccggaacccg
    25261 cgcccgccag caccaggcag gggccggtaa cgaattcgac agcttgttgt tggccggggt
    25321 ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg gaaaataaca gcgtggtagt
    25381 atagccagcc tcatccttac cactcaaggc acgatcatgg caaaaacagc agcagcactg
    25441 catatccttg taaaagaaga gaaactggct ctggatcttc tcgagcagat caagaacggt
    25501 gccgatttcg gcaagctggc gaagaaacac tccatttgcc cgtcaggcaa acgcggcggt
    25561 gatttgggtg aattccgcca gggtcaaatg gttccggcgt tcgataaagt ggttttctct
    25621 tgtccggtac tggagccaac cggcccgctg catacccagt tcggttacca catcattaag
    25681 gtgctgtacc gtaactaaca gcaaggcctt ctccgggaga aggccttgaa ctctgaatcg
    25741 ctattaaccc gcaacagcaa tacgtttcat atccgtcata tagccgcgta gtttcttacc
    25801 tacctgctca atcgcatggc tgcgaatcgc ttcatttaca tcacgcagct gcgcgttatc
    25861 taccgcacct tccggaatag ctttacccaa gtcgcccggt tgcagctctg ccataaacgg
    25921 tttcagcagc ggcacacaag cgtaagagaa cagatagtta ccgtactcgg cagtatcgga
    25981 gataaccacg ttcatttcgt acagacgctt acgggcgatg gtgttggcaa tcagcggcag
    26041 ttcgtgcagt gattcatagt aagcagattc ttcgatgatg ccggaatcaa ccatggtttc
    26101 aaacgccaac tcaacgcctg cttttaccat cgcgatcatc agtacgcctt tatcgaagta
    26161 ctcctgttcg ccgattttgc cttcatactg cggcgcggtt tcgaatgcgg ttttgccagt
    26221 ctcttcacgc caggtcagca gtttcttatc gtcgttggcc cagtcagcca tcatgccgga
    26281 ggagaattcg ccggagataa tgtcgtccat atgtttctgg aacagcggcg ccatgatctc
    26341 tttcagttgc tcagagagcg cgtaagcacg cagtttcgcc gggttagaaa gacggtccat
    26401 catcagggtg atgccgccct gtttcagcgc ttcggtgatg gtttcccaac cgaactgaat
    26461 cagtttttct gcgtatgccg gatcggtgcc ttcttccacc agcttgtcga agcacagcag
    26521 agaaccagct tgcaacatac cgcacaggat ggtttgctcg cccatcaggt cagatttcac
    26581 ttccgcaacg aaagaggatt ccagcacgcc cgcacggtga ccgccggttg cagccgccca
    26641 tgctttagcg atcgccatgc cttcgccttt cggatcgttt tccgggtgaa cggcaatcag
    26701 cgtcggtacg ccgaatccac gtttgtactc ttcacgtagt tcggtgccag ggcatttcgg
    26761 cgcaaccatt acgacggtga tgtctttacg gatctgctcg cccacttcta cgatattgaa
    26821 accatgagag taacccagcg ccgcgccgtc tttcatcagt ggctgtacgg tgcgcactac
    26881 atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga tccgcctgcg ggatcagttc
    26941 ttcgtaagta cccactttaa aaccattttc ggttgcttta cgccaggatg cgcgcttctc
    27001 agcaatcgct tctttacgca gagcgtagga gatatcgaga ccagaatcac gcatgttcag
    27061 gccctggttc agaccctgtg cgccacagcc gacgatgact acttttttac cctgaaggta
    27121 gctcgcgcca tcggcgaatt catcacgccc cataaagcga catttgccca gctgtgccaa
    27181 ctgctggcgc agattcagtg tattgaagta gttagccatg gtgattcctc gtgatgttgt
    27241 gcttcttatt gttcggttcg cgtttgcgag attgaattca ctatatgaca ggaaatttat
    27301 tgcggaaatt gatatattca caacgtcaca ttgcaatttc tgcaacgtca acattaaggg
    27361 ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca tctggcggaa agccgccatt
    27421 ttggccgcag cgcgcgggca atgcacgtta gcccgtccac gctctctcgg cagattcagc
    27481 gcctggaaga cgatctcggt cagccgctgt ttgtgcgcga taaccgcacg gtaacgctaa
    27541 ccgaagcagg cgaagaactg cgcgttttcg cccagcaaac gctgttgcag tatcaacagc
    27601 tgcgccacac catcgatcag caaggaccgt cgctctctgg cgaattacat atcttctgct
    27661 cggtgaccgc tgcctacagc catctgccgc cgattctcga tcgcttccgc gcggaacacc
    27721 cgtcggtgga gattaaactc acgactggtg atgcggcaga tgcgatggaa aaggtcgtca
    27781 ctggcgaagc ggatctggcg attgccggga aaccggagac tctgcccggc gcagtggcgt
    27841 tttcgatgct ggagaatctg gcagtagtgc tgattgcccc cgcgctaccc tgcccggtgc
    27901 gtaaccaagt ttcggcagag aagccggact ggtctacagt gccgtttatc atggccgatc
    27961 agggaccggt gcgccggcgc attgaactgt ggtttcgacg caacaaaatc agcaacccga
    28021 tgatttacgc cacggtcggc gggcatgaag cgatggtgtc gatggtggcg cttggctgtg
    28081 gcgtagcctt gttgccggaa gtggtgctgg aaaacagccc ggaaccggtg cgtaaccgcg
    28141 tgatgatttt agagcgcagt gatgagaaaa cgccgtttga actgggcgtg tgtgtgcaga
    28201 aaaagcggct acatgagccg ctgattgagg cattctggaa gattttgccg aaccattaac
    28261 ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc gtggcaatcg taacccaact
    28321 cattcaaccg ggtttcgaaa tccggttcat ggtcgccaag ttcgaacgcc gccagtacgc
    28381 gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga aatgttccag tgcgtaccca
    28441 gcgtgttgag gaagcgcaac agcgcgcccg gcgattccgg gaattcgaag ctgtagaggc
    28501 gttcctgcaa cggatgcgac ggacgcccgc cgaccatata gcgcacatgc agcttcgcca
    28561 tttcgtcgtc ggagagatcc actacgctgt agccgccatc gttgagcatc tgcaaaattt
    28621 ctttgcgctc ttcgagacca cggctcaggc gcacaccaac aaaaatgcag gcgtttttgg
    28681 catcggcaaa acggtagttg aactcggtga ccgagcgccc gccaagcagc tggcagaatt
    28741 tgaggaagct gcccttttct tccggaatgg tcaccgccag taacgcttca cgctgttcgc
    28801 ccagttcgca gcgttccgag acgtagcgca gaccgtgaaa gttcacgttg gcaccggaaa
    28861 gaatatgcgc cagccgttca ccgcgaatgt tgtgttgggc gatgtatttt ttcatccccg
    28921 ccagcgccag cgcgccagag ggttccgcta ccgcgcgcac atcttcgaac aggtctttca
    28981 tcgccgcaca tatggcatcg ctatcgacgg tgatgatgtc gtcgagatac tcctggcata
    29041 aacggaaggt ttcgtcgccg atgcgtttca ccgcaacacc ttcggcaaac agccccacgc
    29101 gtggcagatc aaccggatga cctgcatcca gcgccgcttt caggcaggcg gagtcttccg
    29161 cttccacggc aatcactttg atttgcggca tcagttgttt gatcagtacc gccacgcccg
    29221 ccgccagacc gccgccgccg acgggtacaa atacgcggtc gagatgggcg tcctgctgga
    29281 gcagttccag cgccagcgtg ccttgcccgg cgatcaccat cggatggtcg aacggcggca
    29341 cccaggtgaa gccctgctgc tgcgacagtt caatcgcttt ggctttcgct tcatcaaaat
    29401 tcgcgccatg aagcagcact tcgccgccaa agccgcgcac cgcatcaact ttgatgtcgg
    29461 cggtggcggt tggcatgacg atcagtgcct tcacgcctaa ccgtgcggaa gaaaacgcga
    29521 cgccctgcgc gtggttaccc gcagaagcgg taatcacgcc gtgcgctttt tgttcttccg
    29581 tcaggcccgc catcatggcg tatgcgccac gaagcttaaa gctgtgcact ggctggcgat
    29641 cttcgcgctt caccagaatc acgttatcaa gacgcgacga cagtttttcc attttctgta
    29701 gcggcgtgac ctgcgccgct tcgtagaccg gcgcgcgcag caccgctctt aaatattcgg
    29761 caccttcagg ggcaccagac aggggttgtg agtctgccat cattaacccc ccagtttcga
    29821 tttatcgcgc accgcacctt tgtcggcgct ggttgccagg ctggcgtagg cacgcagcgc
    29881 aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct ttgtcacccc gagcttcctg
    29941 cgcttcacga cgcgccgcca gttcggcatc gcttacctgt aactgaatgc cacggttcgg
    30001 aatgtcgata gcgataagat cgccgtcttc aatcaggcca atgctgccgc cgcttgccgc
    30061 ttccggtgag acgtggccga tagaaagacc ggacgtgccg ccggagaagc gtccgtcggt
    30121 gataagcgcg caggctttac cgagacccat tgatttcagg aaactggttg ggtagagcat
    30181 ttcctgcatt cccggaccgc ctttcgggcc ttcatagcga attacaacca catctccggc
    30241 gacaacttta ccgccgagaa tcgcttctac cgcgtcgtcc tggctttcgt agactttcgc
    30301 cgggccagtg aatttgagga tgctgtcatc gacgcctgcg gttttaacga tgcagccgtt
    30361 ttctgcgaaa ttaccgtaga gcaccgctag gccgccgtct ttgctgtagg cgtgttccag
    30421 cgagcggata cagccattgg agcgatcgtc gtccagcgta tcccagcggc aatcctgcga
    30481 gaatgcctgt gtagtacgaa tgcccgccgg gccagcgcgg aacatatttt ttaccgcatc
    30541 atcctgggtc acgatgatgt cgtattgttc cagcgtttgc ggcaacgtca ggccaagtac
    30601 atttttcaca tcacggttca gtaaccccgc acgatccagc tcgccgagaa taccgataac
    30661 accaccagca cggtgaacat cttccatatg gtatttttgg gtgctcggcg caactttaca
    30721 cagctgcggt actttgcggg agagcttatc gatatcactc atggtgaagt cgatttccgc
    30781 ttcctgcgcc gccgccagta ggtgaagaac ggtgttggta gagccgccca tcgcgatatc
    30841 cagcgtcatg gcgttttcaa acgccgcctt actggcgatg ttacgcggca gtgcgctttc
    30901 atcatcctgt tcgtagtaac gtttggtcaa ttcaacaatg cgtttaccag cattgaggaa
    30961 cagctgctta cggtccgcgt gggttgccag cagcgagccg ttgcccggct gcgacaggcc
    31021 cagtgcttcg gtcaggcaat tcatagagtt agcggtaaac atcccggagc aagatccgca
    31081 ggttggacag gcggaacgtt caacctgatc gctctgggag tcagaaactt tcgggtctgc
    31141 gccctggatc atcgcatcaa ccagatcgag cttaatgatc tgatcggaca gtttggtttt
    31201 cccggcttcc atcgggccgc cggaaacaaa gatcaccgga atattcaggc gcagggaagc
    31261 catcagcatc cccggggtga ttttgtcgca gttagagatg cagaccatgg cgtcggcgca
    31321 gtgagcgttg accatatact caacggaatc agcgatcagt tcgcgagatg gcagcgaata
    31381 aagcatcccc ccgtggccca tggcaatccc atcatccacc gcaatggtgt tgaactcttt
    31441 ggcaacgccg ccagccgctt caatttgttc ggcgaccagt ttaccgagat cgcgcagatg
    31501 gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc gcgataatcg gcttaccgaa
    31561 atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga gcacccgcca tattacgacc
    31621 atgagtggtg gtggcggaac ggtacttagg catactttat ttactcccag tgtctgtctc
    31681 gtaaatggga cggtgcgtgc cgtcccattt tttgtattta ttgattaact tgatctaacc
    31741 agccccattt atcttcggtt tcgccagtga agaggccgaa gaaggcttgc tgaatgcgtt
    31801 tggtaaccgg gccacaacgg ccttcgccaa cctgaatacc gtctacgctg cgcactggcg
    31861 tgatttctgc cgccgtaccg gacataaaca cttcatccgc caggtacagg gattcgcgcg
    31921 acagcacctg ctcacgcact tcaattccca gctcttttgc cagtttgatg atggcatcac
    31981 gggtaatacc cggcagcgcg gatgaggtga acggtggggt gaacagcacg ccgtctttca
    32041 cttcaaacag gttttcgcct gcgccttcag agatgtaacc attcacatcc aacgcgatac
    32101 cttcctgata accgtggcgg cgcgcttcgc tacccaccag cagggaagag aggtagttac
    32161 caccggcttt tgccgccgtc gggatggtgt ttggtgcagc acggttccag gaggaaacca
    32221 tcgcatcgat cccctgctcc agcgcttctg cgcccagata cgccccccac gggaaagcgg
    32281 cgataatcac atcggttgag tatcccgctg gcgggtttac gcccatgcca acatcaccga
    32341 cgaaaatcag cggacggata taggcgctgg tgagattgtt tttgcggatc acgtcgcgac
    32401 aagcttccat cagctcatca atgctctgcg aaaccgggaa acgatagatt ttggcggagt
    32461 catgcagacg ctgcatatgc tcacgatggc ggaatacaac cggtcctttg tgcgagtcgt
    32521 agcaacggat gccttcaaaa accgaggtgc catagtgcag cgcgtgcgac atcacatgca
    32581 ccttcgcgtc ttcccagcga accatctccc cattgaacca aatgtaatca gctttcttcg
    32641 tggtcatttt tatattcctt ttgcgctcag gcgcggattt gttgtgatgt ggttgtgctc
    32701 tggcagatag caacgtgtgc gacgtccacc agtttattta actgactaaa cagtaagtcg
    32761 accgaccgtg ggctggcaac ggtcaattcg atatttatat tttgtgcatc gctggcggcg
    32821 gccatattca ttgagcagac gtggaaacca cgatgacgca ccacgcgtaa aacacgttct
    32881 aaggtttccg gattgaagcg agccgataca ttgacctgat gttgcatcat gataatttct
    32941 ccaacatttc tgaattactg gcaccaggcg gcaccagcgg ccagacgttc tcaagttcgt
    33001 cgattgagac atgaagcagg tatggcccat cactgttcag catggtgtcg agtgccgctt
    33061 caacctggtc tttatgggtg atgtgttggc cagggatgcc gaaggcgctg gctaacatga
    33121 ggaaatcggg gttatcggta agggtggttt cgctgtatcg ttcctggaaa aacagttgct
    33181 gccattgtcg aaccatccct aaccgttggt tatcgagtaa gacgattttc aacggtaact
    33241 gcttgcgttt tacggtgccc agctcttgca cattcatcat gaaagagccg tcaccggaga
    33301 tacagacgac agtatcgttc ggtcgcgcga cttgtgcgcc aaccgccgcc ggtaaaccga
    33361 aacccatggt gcctaagccg ctggaggtga tgaagttttc cgggcgagtg tggacgatgt
    33421 gctgggcagc ccacatctgg tgctgcccca catctgtggt cacgatgcaa tccgcaggtt
    33481 tacgatccga cagttgtttt aacaacaacg gcgcgtagat agcgtcacca ggatggtcgt
    33541 aacgccaggc atgttcatca cgcagttgcg cgcagtgttg ctgccagtca tcgatattta
    33601 acggctgctg taatgctggt aacagagcat ttaaatcacc ctgtaatgcc acatgtgcct
    33661 gacgcagctt gttcatttct gccggatcga tatccatatg gataacgctg gcgtgtggtg
    33721 cgaaggtgtt cagtttgcca gtcacccggt catcaaaacg tgcgcccacg gcgatcagta
    33781 gatcgcactc ctgcactgcg aagttcgccg ctttggtgcc gtgcatcccc agcatgccca
    33841 gatagtacgg ataatctgct tctactgcgc ccagcccttt caacgtacag gtggcaggca
    33901 tttttgtggc agcgagaaat tcacgcaaag ccgaaactgc ctgcgccatg cccacgccac
    33961 cgccaacgta cagcatcggt ttttgcgctt ttgccagcat ctgacgcgct tgctcgactt
    34021 cggcatgtga gaaagtcact tcgttttcaa cagtggtgaa ccacggttcc agatcgccgc
    34081 tggctaactg gatatctttt ggaatatcga ccaaaaccgg accaggacga ccagagctgg
    34141 cgacgtcaaa cgcttcggcc atgatacgcg gtaactcttc cagtgattgc accaggaaac
    34201 tgtgcttggt gcaggctaac gacaacccca gaatatccac ctcctgaaat gcgtcagtgc
    34261 cgataaacgg tgcggatacc tgaccggtga tagcaacaac ggggatggaa tctaacagcg
    34321 cgtccgcaag cccggttatc aggttggttg cacctggacc agacgtggcg atgcaaacgc
    34381 cagttttgcc ggtagcacgg gcataaccga tagccgccat tgctgcacct tgctcgtgtc
    34441 ggcacagcaa gtgttccacg ccaccgtcat acaatgcatc gtaaaccggc ataattgcgc
    34501 cacccggata accgaaaacg gtgttcacac cctgtgcccg caacgcatgt accacccact
    34561 gtgcgccatt catagttagt tccccgtcct gaatcttgag aaacagaatt ttgtgctgtt
    34621 attcattgtc tgctcctcag ttatgttttt aaggtcaaaa aaaacccccg gacctttcgg
    34681 tgcgggggtc ttagttcgtt aaggcttgat ctctaagcct ttcctcgtcc aagtgcagcc
    34741 ccgcacggtg ggataataat caccaccacg ctaatcacga ccaggctaat cactcgtaga
    34801 agggctgtca ttttgtcttt tcttgcatct tgttcgaagg aatgcctaaa gagttaccat
    34861 agattttgcg aatagcacaa gatatttttt ggcc
//
LOCUS       DAZFJL010000034        33896 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFJL010000034 DAZFJL010000000
VERSION     DAZFJL010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.034"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            20..847
                     /locus_tag="ACHJ8C_001662"
     CDS             20..847
                     /locus_tag="ACHJ8C_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO5551529.1"
                     /translation="MLLIPCIGNAQEIKIDESWLHQSLNVIGRTDSRFGPRLTNDLYP
                     EYTVAGRKDWFDFYGYVDLPKFFGVGSHYDVGIWDEGSPLFTEIEPRFSIDKLTGLNL
                     AFGPFKEWFIANNYVYDMGDNQSSRQSTWYMGLGTDIDTGLPIKLSANIYAKYQWQNY
                     GAANENEWDGYRFKIKYSIPLTNLFGGRLVYNSFTNFDFGSDLADKSHNNKRTSNAIA
                     SSHILSLLYEHWKFAFTLRYFHNGGQWNAGEKVNFGDGPFELKNTGWGTYTTIGYQF"
     gene            complement(1065..3497)
                     /gene="metL"
                     /locus_tag="ACHJ8C_001663"
     CDS             complement(1065..3497)
                     /gene="metL"
                     /locus_tag="ACHJ8C_001663"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO5551530.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADNLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPSAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(3500..4660)
                     /gene="metB"
                     /locus_tag="ACHJ8C_001664"
     CDS             complement(3500..4660)
                     /gene="metB"
                     /locus_tag="ACHJ8C_001664"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO5551531.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQSDEQALRAALAENPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEHTLRRFLGELSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4937..5254
                     /gene="metJ"
                     /locus_tag="ACHJ8C_001665"
     CDS             4937..5254
                     /gene="metJ"
                     /locus_tag="ACHJ8C_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO5551532.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            5314..5922
                     /gene="yiiX"
                     /locus_tag="ACHJ8C_001666"
     CDS             5314..5922
                     /gene="yiiX"
                     /locus_tag="ACHJ8C_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO5551533.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDSSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(5983..6195)
                     /gene="rpmE"
                     /locus_tag="ACHJ8C_001667"
     CDS             complement(5983..6195)
                     /gene="rpmE"
                     /locus_tag="ACHJ8C_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO5551534.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            6398..8596
                     /gene="priA"
                     /locus_tag="ACHJ8C_001668"
     CDS             6398..8596
                     /gene="priA"
                     /locus_tag="ACHJ8C_001668"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO5551535.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGSRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILASPLADEKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            8752..9777
                     /gene="cytR"
                     /locus_tag="ACHJ8C_001669"
     CDS             8752..9777
                     /gene="cytR"
                     /locus_tag="ACHJ8C_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO5551536.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            9869..10828
                     /gene="ftsN"
                     /locus_tag="ACHJ8C_001670"
     CDS             9869..10828
                     /gene="ftsN"
                     /locus_tag="ACHJ8C_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO5551537.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            10921..11451
                     /gene="hslV"
                     /locus_tag="ACHJ8C_001671"
     CDS             10921..11451
                     /gene="hslV"
                     /locus_tag="ACHJ8C_001671"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO5551538.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            11461..12792
                     /gene="hslU"
                     /locus_tag="ACHJ8C_001672"
     CDS             11461..12792
                     /gene="hslU"
                     /locus_tag="ACHJ8C_001672"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO5551539.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            12859..13785
                     /gene="menA"
                     /locus_tag="ACHJ8C_001673"
     CDS             12859..13785
                     /gene="menA"
                     /locus_tag="ACHJ8C_001673"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO5551540.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGAVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            13878..14363
                     /gene="rraA"
                     /locus_tag="ACHJ8C_001674"
     CDS             13878..14363
                     /gene="rraA"
                     /locus_tag="ACHJ8C_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO5551541.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(14448..14693)
                     /gene="zapB"
                     /locus_tag="ACHJ8C_001675"
     CDS             complement(14448..14693)
                     /gene="zapB"
                     /locus_tag="ACHJ8C_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO5551542.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            15118..15963
                     /gene="glpF"
                     /locus_tag="ACHJ8C_001676"
     CDS             15118..15963
                     /gene="glpF"
                     /locus_tag="ACHJ8C_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO5551543.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCDAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            15986..17494
                     /gene="glpK"
                     /locus_tag="ACHJ8C_001677"
     CDS             15986..17494
                     /gene="glpK"
                     /locus_tag="ACHJ8C_001677"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO5551544.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            17665..18675
                     /gene="glpX"
                     /locus_tag="ACHJ8C_001678"
     CDS             17665..18675
                     /gene="glpX"
                     /locus_tag="ACHJ8C_001678"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO5551545.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            18772..19518
                     /gene="fpr"
                     /locus_tag="ACHJ8C_001679"
     CDS             18772..19518
                     /gene="fpr"
                     /locus_tag="ACHJ8C_001679"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO5551546.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLERFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(19523..19951)
                     /gene="uspD"
                     /locus_tag="ACHJ8C_001680"
     CDS             complement(19523..19951)
                     /gene="uspD"
                     /locus_tag="ACHJ8C_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO5551547.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIIQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(19978..20277)
                     /gene="yiiS"
                     /locus_tag="ACHJ8C_001681"
     CDS             complement(19978..20277)
                     /gene="yiiS"
                     /locus_tag="ACHJ8C_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO5551548.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(20489..20929)
                     /gene="yiiR"
                     /locus_tag="ACHJ8C_001682"
     CDS             complement(20489..20929)
                     /gene="yiiR"
                     /locus_tag="ACHJ8C_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO5551549.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            21030..21629
                     /gene="yiiQ"
                     /locus_tag="ACHJ8C_001683"
     CDS             21030..21629
                     /gene="yiiQ"
                     /locus_tag="ACHJ8C_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1454 family protein"
                     /protein_id="HFO5551550.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQITWLPIQGPEQKAAKAKAQAYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            21737..22504
                     /gene="tpiA"
                     /locus_tag="ACHJ8C_001684"
     CDS             21737..22504
                     /gene="tpiA"
                     /locus_tag="ACHJ8C_001684"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO5551551.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(22559..23314)
                     /gene="cdh"
                     /locus_tag="ACHJ8C_001685"
     CDS             complement(22559..23314)
                     /gene="cdh"
                     /locus_tag="ACHJ8C_001685"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO5551552.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEQCLP
                     NQQENQNPSPCAEVKPNAGYVVLKDRHGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSQKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(23421..24410)
                     /gene="sbp"
                     /locus_tag="ACHJ8C_001686"
     CDS             complement(23421..24410)
                     /gene="sbp"
                     /locus_tag="ACHJ8C_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO5551553.1"
                     /translation="MKKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(24730..25692)
                     /gene="pfkA"
                     /locus_tag="ACHJ8C_001687"
     CDS             complement(24730..25692)
                     /gene="pfkA"
                     /locus_tag="ACHJ8C_001687"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO5551554.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIELL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(25873..26775)
                     /gene="fieF"
                     /locus_tag="ACHJ8C_001688"
     CDS             complement(25873..26775)
                     /gene="fieF"
                     /locus_tag="ACHJ8C_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO5551555.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(27012..27230)
                     /gene="ogrK"
                     /locus_tag="ACHJ8C_001689"
     CDS             complement(27012..27230)
                     /gene="ogrK"
                     /locus_tag="ACHJ8C_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFO5551556.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(27312..28475)
                     /locus_tag="ACHJ8C_001690"
     CDS             complement(27312..28475)
                     /locus_tag="ACHJ8C_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO5551557.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKVPQVTITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(28475..28954)
                     /locus_tag="ACHJ8C_001691"
     CDS             complement(28475..28954)
                     /locus_tag="ACHJ8C_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5551558.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAFQFLG
                     VGDENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISRVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATKFTGMTGAG"
     gene            complement(28969..31416)
                     /locus_tag="ACHJ8C_001692"
     CDS             complement(28969..31416)
                     /locus_tag="ACHJ8C_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5551559.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASRSLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAVVGINTRNLARDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDAAAIQAA
                     TPVTLNMALANQRSMEENAQLLLGTKASFQLSNDDVSHVGDVLSATMNKSAADFQGLS
                     DALTYLGPVARTAGVSLEQAAAMTGVLHDNNIRGSMAGTGSSAVVTRLQAPTGKAWDA
                     LKELGVKTSDKKGNMRPLFTILKEIQASFDKHKLGTSQKGEYLKTIFGEEALKSANVL
                     LAAAASGKLDKLTATLKASDGKTEELVKIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRNLTQTATKYVLKLDGWIQKNKSLASTIGLIAGGALALTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTQAATYGSG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(31409..31564)
                     /locus_tag="ACHJ8C_001693"
     CDS             complement(31409..31564)
                     /locus_tag="ACHJ8C_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO5551560.1"
                     /translation="MTFPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGNTNE"
     gene            complement(31561..31836)
                     /locus_tag="ACHJ8C_001694"
     CDS             complement(31561..31836)
                     /locus_tag="ACHJ8C_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO5551561.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(31893..32411)
                     /locus_tag="ACHJ8C_001695"
     CDS             complement(31893..32411)
                     /locus_tag="ACHJ8C_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO5551562.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(32424..33614)
                     /locus_tag="ACHJ8C_001696"
     CDS             complement(32424..33614)
                     /locus_tag="ACHJ8C_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFO5551563.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVTSTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIHDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            33694..>33896
                     /locus_tag="ACHJ8C_001697"
     CDS             33694..>33896
                     /locus_tag="ACHJ8C_001697"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5551564.1"
                     /translation="MDETTDKEKADNPPACHDLYTLIFRLTIYITQKLSNLTVCFEQE
                     ADICLSGYMPLCGYYPESIIHLS"
BASE COUNT         8123 a         9112 c         8670 g         7991 t
ORIGIN      
        1 gcactggata gctttatcca ttctattaat accttgcatt ggaaacgctc aggaaattaa
       61 aattgatgaa agctggttac atcaaagctt gaatgtcatt ggtcgcacag actctcgctt
      121 tggcccaaga ctgactaacg acctctaccc tgaatatact gtagcaggaa gaaaagactg
      181 gtttgatttt tatggttatg ttgatctacc gaaattcttt ggcgtcggca gtcactatga
      241 tgttgggatc tgggatgaag gctcaccact atttacggaa atagaacctc ggttttccat
      301 tgacaaattg accggattaa atcttgcgtt cggcccattt aaagaatggt tcattgcaaa
      361 caactatgtc tatgatatgg gtgacaacca gtcatcccgg caaagtacat ggtatatggg
      421 gcttggtaca gatatcgaca cgggtctacc aattaagctt tctgccaata tatacgccaa
      481 gtatcagtgg caaaactatg gtgccgctaa tgaaaatgaa tgggacggat atcgattcaa
      541 aataaaatat agcatccctc ttacaaattt attcggaggg cgattggtat acaatagttt
      601 tactaacttt gattttggct ccgatcttgc ggacaagtca cacaataata aacgaaccag
      661 taatgctatt gcttcaagcc atatcctttc ccttctatat gaacactgga aatttgcatt
      721 tacactacgt tattttcaca acggtggaca atggaatgcg ggagagaagg ttaacttcgg
      781 agatggtcca tttgaattaa aaaatacagg atggggaacc tatactacta ttggttatca
      841 attttaaatt aggtgacatt ttagatattg ttacttaatg tcattttgta attgtcatta
      901 ataacttcac cattcactct gttacttcat cgctcaacac aaaagccccc ttattaccgg
      961 gggcactgac caaaggcaaa atgacaatca atcaccactt tcccaggaag aggtttcggg
     1021 gtgacaacaa aagctggcaa ctgccagctt ttgttcaata atcactacaa caactgcgcc
     1081 agccggttaa tatccgactg aatcgccccg gcggtgacgt cgcgccctgc acctggcccg
     1141 cggatcacca gcgggttgtc gcgataccag cggctttcga tggcaaagac gttatcgcat
     1201 ggcagtagtg atgccagcgg atggtcttca cgcaccgctt ccacgcccac acgcgctttg
     1261 ccgttggcat cgaaacgcgc cacgtagcgc agcaccagcc ccatttcgcg ggccgcttcc
     1321 agtcgttgca ccatctgctc attaagttca tcgccatttt caaagaaatg gtcgatgctg
     1381 ccgccttcgc aatgggcagg caccagtgat tcaacacgta cctggtccgg ttcaatgttg
     1441 taacccgctt cacgcgccag aatcaccagc ttgcgcatca cgtctttgcc ggaaaggtca
     1501 tcacgcgggt caggttcggt taagccctgc tgccacgcct gatccaccag ctcggtaaac
     1561 ggcacgctac cgtcgaattg caggaacagc caggagagcg tgccggagaa gatcccactg
     1621 atcgacaaaa tagtatcgcc gctgtcgatc agatcgcgca cggtgtggtt gatcggcaag
     1681 cctgcgccga cggtagcgtt gtacagccag tgacgcccgg ttttttcgaa ggcgtcgtgg
     1741 atctggcgat atttattgct gtcgctggct cccgccagtt tgttggcgct gatgacgtgg
     1801 aaaccgtggc tggcaaaatc gagatactga tcagcaagct gctggctggc ggtaacgtcc
     1861 aacaccacta aatcatcata cggatgggcg cgcatccaca ggaacagcga ctcttcatcc
     1921 tgctcgaccg cttcatcgtt gaagaaggct aacgcgcggc tggcgtccag gccgtcatag
     1981 ctcaacaggc tgcggcggct gtccaccaca cctgccagca caaattcaaa gccggtacgt
     2041 gccgaaagcg tgctctgctc acgggcgaac agttccagcc agcgggaacc gatattgccc
     2101 ttgccgaaca ataccaggcc gatgcgtttt tccgcacgga agacggactg atgcagcccc
     2161 tgaatcaggc tttcggtcgg gccggtgcgc agtactgcca ccaggctgat gccatcatca
     2221 gactgccagg taaactcgac cggctggcct ttcagttgct gccagaagcg gtggcaatgc
     2281 agcgggttac gggtgacgcc cgccccaacc atcgccacca gcgccagccc ctgacgcagg
     2341 cgcagttcgc caggtaatcc cgcttcgtcg aggattttca gcgcactgtc ggccacttct
     2401 gaggtgtagc aaaattgcag caactggcga tcgttatgca cgccaaccgc cagtgggcgc
     2461 acctgagcac gtttcaggat ctggtcgatc tctttatgcg ccagtttgaa atcctggctg
     2521 gcgggcacct gaaactcaat caagcagaca tcatcatggc tggtgacaat acgcgcacca
     2581 gtaccggagg ccagcacgcg ttcaatgcgc gtggaacctt gatccggcgt gtagctacag
     2641 cgcagttgca ggtcgatttc gctgccagaa acgggctgta aagtacgggc atgaagaacg
     2701 ggagccgcca gacgcgccag ttcgctggct tcatccagac gcagcaacgg cagcaggcag
     2761 gcatctttca ctttacgcgg gtcggcgcta tataccccgg cgacgtcgct ccagatggtt
     2821 acgcgagaaa cacccgccag cgcaccgatt tgtgtcgcgg aatagtcgga accgttacgc
     2881 ccaagcagca ccgtttcacc ggcgttgtta cggctgataa acccggtcac caccagacgt
     2941 ttgcccggat gttgtaccag tagctgttgc agcaacgggt acgaaagccc ttcatcaacc
     3001 tgcggttgcg cagcgcgttc agcgcgtaaa aactcgcggg catcaagcca ggccgatggc
     3061 agcccttgtt gattaagcac cgcagacatc aggcgcgccg accacacttc accgtggccc
     3121 accacttccg catacaccgc atcgttaata ccgctgtcga gcaacgccgc caggcgctca
     3181 agatcgctga caaaagcgct aatgaggtta tcggcttctt cagcgggtag caggccgcta
     3241 atcagatcgc actgataacg acgtaatgtt tgttgaacct gatgcgcaga gagacgatcg
     3301 gtctggctta gtttcaacca gttaatcaac tggttagtgg tgctaccggc ggcggaaacc
     3361 accatcatat cgtcaggctg agagtactcc gccataatgc ccgcgacacg caaataacac
     3421 ttcacatcag ccagactact gccaccaaat ttatgcagtt gacgaccttt cgcccctgcc
     3481 tgcgcaatca cactcatttt taccccttgt ttgcagcccg gaagccattt tccaggtcgg
     3541 caattaaatc ttcgccatct tcaataccgg tggagatacg cagcagcgtc tcggagatcc
     3601 cggcggcagc acgcgcttct ggcgccatgc ctgcatgtgt catggttgcg gcgtgagaga
     3661 ttaaactttc cactccccct aatgattccg ccagcgtaaa caacgacagc tcgcccagga
     3721 aacgacgcag cgtgtgctca tcgccatcca gttcaaaact caacattgcg ccaaagcctt
     3781 tttgctggcg cgcggcaatt tcatgcccct gattttccgg cagcgacggg tgatacagtt
     3841 ttttcaccaa cggctgggtt tgcagatatt tcacaatcgc ctgcgcgttg cgctgggcca
     3901 gctccattcg cggcaccagc gttcgcaacc cgcgcagcag cagatagctg tcaaacgcgc
     3961 cgcccgtcac gccaatattg tttgcccacc aggcaagttc agtgacaacg tccgggtctt
     4021 tagcaatcac tacgcctgcc accacgtcag agtgaccgtt cagatatttc gtgcatgaat
     4081 gcaacaccag atcggcacct aatgccagtg gattttgtaa tgccgggctt aagaaggtgt
     4141 tatccaccac gctcaccgcc ccgacttccc ttgccagatg gcagattttc gcaatatcca
     4201 cgacgcgtaa caatggatta cttgggcttt ctaccagtac cagtttgggg ttttctgcca
     4261 gcgctgcccg taatgcctgt tcatcgcttt gatcaacaaa caacacgcga tagcaaccgc
     4321 gtttcgccag gctgtcgaac agacgatagc taccgccgta gcagtcgtgc ggcgcaacca
     4381 gcagatcgcc aggtttcaaa aagacggtcg ttaccaggtg aatcgcggac atgccggtat
     4441 tagtaagtac tgcacccgca ccgccttcca gttctgccag cgcacgctga accacatcgc
     4501 gcgttgggtt gccgcgccgc gagtaatcat gcgcgcgcgg ttcattaaat ccggtaaaat
     4561 tataggtact ggaaagatgg atgggaggga caacgcaacc atactgttcg tcgtcattta
     4621 acccgctacg cactgcgatg gtggcctgtt tacgcgtcat gtgatgaagt tccctgggct
     4681 tggtcggtga aatgtcaggc accagagtaa acattgtgtt aatggacgtc aatacatctg
     4741 gacatctaaa cttctttgcg tatagattga gcaaatctca aatagccgtt aaaattatat
     4801 gcattatcac gccgacaggt gcattacacg atgtcacggt aacgcctgta cggtaaacta
     4861 tgcgggttta cggtcagtac ccatattaat tgtgtggtct ggcctcaatt tattgacgaa
     4921 gaggattaag tatctcatgg ctgaatggag cggcgaatat atcagcccat acgctgagca
     4981 cggcaagaag agtgaacaag tcaaaaaaat tacggtttcc attcctctta aggtgttaaa
     5041 aatcctcacc gatgaacgca cgcgtcgtca ggtgaacaac ctgcgtcacg cgaccaacag
     5101 cgagctgctg tgcgaagcgt ttctgcatgc ctttaccggg caacctttgc cggatgatgc
     5161 cgacctgcgt aaagagcgca gcgacgaaat tccggaggcg gcaaaagaga tcatgcgtga
     5221 gatggggatt aacccggaga cgtgggaata ctaaagcgga aaagagcggc gcggagttaa
     5281 ccagccgctt ttttcataca tggacgttta acgatgaaaa tcaggctact gattctcagc
     5341 ctgctggttt ctgtgcctgc atttgcctgg caaccacaaa ccggcgacat catctttcag
     5401 atctctcgct catcgcaaag taaagcgatc caactggcga cccattccga ttacagccac
     5461 accggtatgc tggtgatacg caacaaaaag ccctacgttt ttgaagcggt cggcccggta
     5521 aaatacaccc cactcaagca gtggatcgcc catggtgaaa agggcaaata cgttgttcgc
     5581 cgcgttgaag gcggactgag tgttgaacaa cagcaaaaac tggcgcaaac ggcaaaacgt
     5641 tatctcggta aaccgtatga ctccagcttc tcgtggagcg acgatcgcca gtactgttcg
     5701 gaagtggtgt ggaaggtcta ccagaatgcg ctgggaatgc gtgtgggcga gcagcagaag
     5761 ctgaaagagt tcgatcttag taacccgctg gttcaggcaa agctcaaaga acgctacggc
     5821 aaaaatattc cgcttgagga aacggtagtt tcaccacaag ccgttttcga tgcgccacaa
     5881 ctcaccacgg tcgccaaaga atggccgctg ttttcctggt aatacgacca aaattcaggc
     5941 acaaaaaaag cgccgtacgg cgcttttttc ggaaatccgg tcttatttgc tgcccgggat
     6001 gttgaaacgc ttgttgaagc ggtcaacacg gccaccggta gcaacatcac gctgtttgcc
     6061 agtgaagaac gggtggcact tgctgcacac gtcgaggttc aggtcatgac caacggtgga
     6121 gcggattttc attacgttac cgcaagagca gctagcagta atttcttcgt atttcgggtg
     6181 aatatctttt ttcatgggaa aacctcggtt taaggccgcg tcgctcttcc agccctaacg
     6241 ccagacacca cgcgatgtta aaagtatagc ttcaatacga tcatttcgta cgaaggcgcg
     6301 aaatcataca gaaattaacc agcgtatgca aactgatccg cactcttcta cggcaatgtg
     6361 tatactaacc caccgaattt caagtcagga tgatgctatg cccgttgccc acgttgcctt
     6421 gcccgttccg cttcctcgta cctttgacta tctgctgcca gaaggcatga cggttaaagc
     6481 cgggagtcgc gtgcgcgtgc cgtttggcaa acaacaggag cgtatcggtg ttgtggtgtc
     6541 agtgagcgat gtcagtgagc tgccgcttaa cgagctgaaa gcggtagttg aagtgctgga
     6601 cgttgagccg gtatttactc actctgtctg gcgattactg ctctgggcgg cagattacta
     6661 tcatcatccg attggcgatg tgctgtttca tgccttgccg attttactgc gccaggggcg
     6721 gcctgcggcg aacgcgccga tgtggtactg gtttgccact gaacaaggtc aggcggtgga
     6781 tctgaacagc ctgaaacgtt cgccaaagca acaacaggcg ctggcagcgt tacggcaagg
     6841 caaaatctgg cgcgaccagg ttgccacgct cgaatttaat gatgccgcgt tgcaggcact
     6901 gcgcaaaaaa ggtctgtgtg atttagcaag tgaaacacca gagtttagcg actggcgaac
     6961 gaactatgcc gtttctggtg agcggttgcg gttgaatacc gaacaggcca ccgccgttgg
     7021 cgcaattcat agcgcggcag atactttttc tgcctggctg ctggcgggcg ttaccggttc
     7081 cggtaaaacg gaggtttacc tcagtgtact ggaaaacgtg ctcgctcagg gcaaacaggc
     7141 gctggtgatg gtgccggaaa tcggcctgac accgcaaact atcgcccgtt ttcgtgaacg
     7201 ttttaatgcc ccggtggaag ttctgcattc cggcctgaac gacagcgagc gtctttcggc
     7261 gtggttgaaa gcgaaaaatg gcgaggcggc gattgtcatc ggcacccgct ccgcgctgtt
     7321 tacgccgttt aaaaatctcg gcgtgattgt tattgatgaa gagcacgaca gctcctacaa
     7381 gcagcaggaa ggctggcgct atcatgcccg cgacctggcg gtgtatcgcg cgcatagcga
     7441 gcaaatccca attattcttg gctccgcaac gcccgcactg gaaacgttat gcaacgtgca
     7501 gcagaaaaaa taccgcctgc tgcgcctgac ccgtcgggcg ggcaatgcgc gcccggcaat
     7561 tcaacatgtg ctggatttaa aaggtcagaa ggtgcaggca ggtctggctc cggcgttaat
     7621 cacccgtatg cgccagcatt tacaggccga caaccaggtt atcctctttc ttaaccgccg
     7681 gggttttgcg cctgcgctac tgtgccacga ctgtggttgg atagccgaat gcccacgttg
     7741 cgatcactac tacacgctgc atcaggcgca acagcatttg cgctgccacc attgcgacag
     7801 tcagcgtccg gtgccgcgcc agtgcccttc ttgcggttcc acgcacctgg tccccgtggg
     7861 gctgggcacc gaacagcttg aacagacgct cgcgccgttg ttccctggtg tccccatttc
     7921 tcgtatcgac cgcgatacca ccagccgcaa aggggcgctg gaacaacaac tggcggaagt
     7981 acatcgcggt ggcgcgagga ttttgattgg cacgcaaatg ctggcgaaag gtcaccactt
     8041 tccggatgtg acgctggtgg cattactgga cgtggacggc gcgctgtttt ctgccgattt
     8101 ccgctcggcg gagcgttttg cccagcttta cacccaagtg gcaggccgtg ccggacgtgc
     8161 cggtaaacag ggcgaagtag ttctgcaaac gcaccatcct gaacatccgc tgttgcaaac
     8221 gttgctctat aaaggctacg acgcctttgc cgaacaggca ctggctgaac gacgcatgat
     8281 gcagctgccg ccgtggacca gccatgtgat tgtgcgtgcg gaagatcata acaatcagca
     8341 cgcgccattg ttcctgcaac aactgcgtaa tctcattctc gccagcccac tggcagacga
     8401 gaaactgtgg gttctcggtc cggttccggc gctggcccct aaacgtggcg gtcgctggcg
     8461 ctggcagata ttgttgcagc acccttcccg cgtgcgctta cagcacatca tcaatggtac
     8521 gctggcgctt attaatacaa taccggattc ccgcaaggtg aaatgggtgc tggatgtcga
     8581 tccgattgag ggttaaaccg ctcacgatgc gaggcggatc gaaaaattca atattcatca
     8641 cacttttcat gaaaattctg taaccgtttt cacgcgctat ctgctaaaaa tgttgccgat
     8701 gtgaagtaaa catggatgta gtacgcctga cgtgccaggc gaggagtgag tgtgaaagcg
     8761 aagaagcagg aaactgccgc gaccatgaaa gacgttgccc tcaaggcaaa agtctctaca
     8821 gcgaccgtct cccgagcatt aatgaatccc gataaagtct cccaggccac ccgtaatcgg
     8881 gttgaaaaag cggcccggga agtgggttat ttaccgcagc ctatggggcg caacgtcaag
     8941 cgtaatgaat cccgcaccat tctggtgatt gtcccggata tctgcgatcc cttctttagc
     9001 gaaattattc gcggtatcga agttacggcg gcaaatcacg gatatctggt gctgattggc
     9061 gactgtgcgc atcaaaatca gcaggaaaaa acctttatcg atttgatcat caccaagcaa
     9121 attgatggca tgttgttgct gggttcaagg ctgccgtttg atgccagcat tgaggaacag
     9181 cgtaatctgc cgccgatggt gatggcgaac gaatttgcac cggaactgga gctgcctaca
     9241 gttcatatcg acaatctgac cgccgcattt gatgcagtaa attatttata tgagcaaggg
     9301 cataaacgga ttggctgtat agccggtccc gaagagatgc cgctgtgtca ctaccgcctg
     9361 caaggctacg ttcaggcgct gcgtcgctgc ggcattatgg ttgatccgca atacatcgcc
     9421 cgtggcgact tcaccttcga agccggaagc aaagcaatgc aacagctgct tgatcttcca
     9481 caaccgccta ctgctgtctt ctgccatagc gatgtgatgg cgctcggcgc actttcgcag
     9541 gcaaaacgcc aggggctgaa agtaccggaa gacctttcca taatcggttt tgataacatc
     9601 gacctgacgc aattttgtga tccgccgctg acaaccatcg cgcagccgcg ttacgaaatc
     9661 ggtcgggaag ctatgctgtt attgcttgat caaatgcagg ggcaacacgt tggcagtggc
     9721 tctcgtttaa tggactgcga acttatcatc cggggatcaa cacgtgcgtt accttaaagt
     9781 aaacctttaa gactgccgtg actggtcaaa ggcccgccgc ttaagtaaca tggcgggctg
     9841 acgaacgaat aaatacagcg aaacgatagt ggcacaacga gattatgtac gccgcagcca
     9901 accggcacct tcgcggcgaa aaaagagcac ctcacggaaa aagcaacgaa atctgcctgc
     9961 ggtttctccc gctatggtcg ctattgctgc cgccgttctt gtgaccttta tcggtggtct
    10021 gtacttcatt acgcatcaca agaaagaaga gtccgagacg ctgcaaagcc agaaagtgac
    10081 cggaaacgga ctaccaccaa aaccagaaga acgctggcgc tacattaaag agctggaaag
    10141 tcgccagccg ggtgtgcgtg cgcccacaga accttctgcc ggtggtgaag tgaaaacgcc
    10201 ggagcagctg acaccagaac aacgtcagct tcttgaacaa atgcaggctg atatgcgcca
    10261 gcagccaacg cagctggttg aagttccgtg gaatgaacag acgccggaac agcgccagca
    10321 aacgctacag cgccagcgtc aggcgcagca attagcggaa cagcaacgtc tggctcagca
    10381 gtcgcgtacg actgaacaaa gctggcagca gcagacgcgt acgtcgcaag ccgcgccggt
    10441 acaggcgcaa ccgcgccagt ctaaaccggc ttctactcag cagccgtacc aggatctgct
    10501 gcaaactcct gcgcacacga ccgcgcagtc gaagccgcag caagctgcgc cagttgctcg
    10561 tgccgctgac gcgccaaaac cgacggcgga gaaaaaagac gaacgccgct ggatggtgca
    10621 gtgcggttcg ttcagaggcg cggaacaggc agagacggta cgtgctcagc tggcgttcga
    10681 aggctttgac tcgaaaatca ccaccaacaa tggctggaat cgtgtggtca ttggcccggt
    10741 gaaaggcaaa gagaacgcag acagcaccct caatcgcttg aagatggcgg gtcatacaaa
    10801 ctgcattcgg ctcgccgccg ggggttgaaa ccctcaaaat cccccccatc tataattgca
    10861 ttatgccccg tacttttgta cggggtttgt actctgtatt cgtaaccaag gggtcagctc
    10921 gtgacaacta tagtaagcgt acgccgtaac ggccatgtgg tcatcgctgg tgatggtcag
    10981 gccacgttgg gcaataccgt aatgaaaggc aacgtgaaaa aggtccgccg tctgtacaac
    11041 gacaaagtca tcgcgggctt tgcgggcggt actgcggatg cttttacgct gttcgaactg
    11101 tttgaacgta aactggaaat gcatcagggc catctggtca aagccgccgt tgagctggcg
    11161 aaagactggc gtaccgatcg catgctgcgc aaacttgaag cactgctggc agtcgcggat
    11221 gaaactgcat cgcttatcat caccggtaac ggtgacgtgg tgcagccaga aaacgatctt
    11281 attgctatcg gctccggcgg cccttacgcc caggctgcgg cgcgcgcgct gttagaaaac
    11341 actgaactta gcgcccgtga aattgctgaa aaggcgttgg atattgcagg cgacatttgc
    11401 atctatacca accatttcca caccatcgaa gaattaagct acaaagcgta aggatctccc
    11461 atgtctgaaa tgaccccacg cgaaatcgtc agcgaactgg ataagcacat catcggccag
    11521 gacaacgcca agcgttccgt ggcgattgct ctgcgtaacc gctggcgtcg catgcagctc
    11581 aacgaagagc tgcgccatga agtgaccccg aaaaatatcc tgatgatcgg cccgaccggt
    11641 gtcggtaaaa ctgaaatcgc ccgtcgcctg gctaagctgg cgaatgcgcc gttcatcaaa
    11701 gttgaagcga ccaaattcac cgaagtgggc tatgtcggta aggaagtgga ttctattatt
    11761 cgcgatctga ccgatgccgc cgtgaaaatg gtacgcgtcc aggctatcga gaaaaaccgt
    11821 tatcgcgctg aagaactggc agaagaacgt attctcgacg tactgatccc accggctaaa
    11881 aataactggg gacagaccga acagcagcag gaaccgtccg ctgctcgtca ggcattccgc
    11941 aaaaaactgc gtgaaggcca gcttgatgac aaagaaatcg agatcgatct tgccgcagca
    12001 ccgatgggcg ttgaaattat ggctcctccg ggcatggaag agatgaccag ccagctgcag
    12061 tccatgttcc agaacctggg cggtcagaaa caaaaagcgc gtaagctgaa aatcaaagac
    12121 gccatgaagc tgctgattga agaagaagcg gcgaaactgg tgaacccgga agagctgaag
    12181 caagatgcta tcgacgctgt tgagcagcac gggatcgtgt ttatcgacga aatcgacaaa
    12241 atctgtaagc gcggcgagtc ttccggtccg gatgtttctc gtgaaggcgt tcagcgtgac
    12301 ctgctgccgc tggtagaagg ttgcactgtt tccaccaaac atgggatggt caaaactgac
    12361 cacattctgt ttatcgcttc tggcgcgttc cagattgcga aaccgtctga cctgatcccg
    12421 gaactgcaag gtcgtctgcc aatccgcgtt gagctgcagg cactgaccac cagcgacttc
    12481 gagcgtattc tgaccgagcc gaatgcctct atcactgtgc agtacaaagc actgatggca
    12541 actgaaggcg taaatatcga gtttaccgac tccggtatta agcgcatcgc ggaagcggca
    12601 tggcaggtga acgaatctac cgaaaacatc ggtgctcgtc gtttacatac cgttctggag
    12661 cgtttaatgg aagagatttc ctacgacgcc agcgatttaa gcggtcaaac tatcactatt
    12721 gacgcagatt atgtgagcaa acatctggat gcgttggtgg cagatgaaga tctgagccgt
    12781 tttatcctat aatcgcgttc aatcattttc atcattgttt gatggggctg aaaggcccca
    12841 tttttattgg cgcgtattat gactgaacaa caaattagcc gaactcaggc gtggctggaa
    12901 agtttacgac ctaaaaccct ccccctcgcc tttgctgcaa ttatcgtcgg gacggcgctg
    12961 gcatggtggc aaggtcactt cgatccactg gtcgccctgc tggcattgat taccgccggg
    13021 ctattacaga tcctttctaa cctcgccaat gattacggcg atgcggtaaa aggcagcgat
    13081 aaacctgacc gcattgggcc gctacgcggc atgcaaaaag gggtcattac ccagcaagag
    13141 atgaaacggg cgctcattat taccgttgtg ctcatctgtc tttccgggct ggcactggtt
    13201 gcagtggcgt gccatacgct ggccgatttt gtcggtttcc tgattcttgg tgggttgtcg
    13261 atcattgccg ctatcaccta caccgtgggc aatcgtcctt atggttatat cggtttgggt
    13321 gatatttccg tactggtttt ctttggctgg ttgagcgtca tggggagctg gtatttacag
    13381 gctcatacat tgattccggc actgatcctt ccggcaaccg catgcggcct gctggcaacg
    13441 gcagtactga acattaataa cctgcgtgat atcaatagcg accgcgaaaa cggcaaaaac
    13501 acgctggtgg tgcgcttagg cgcagtaaac gcgcgtcgtt atcatgcctg cctgctgatg
    13561 ggctctctgg tgtgtctggc gctgtttaat ctcttttcgc tgcatagcct gtggggctgg
    13621 ctgttcctgc tggcggcacc gttgctggtg aagcaagctc gttatgtgat gcgcgaaatg
    13681 gacccggtgg cgatgcgacc aatgctggaa cgcactgtca aaggagcgtt actgactaac
    13741 ctgctgtttg ttttagggat attcctaagc cagtgggcag cataactgac aaatatcaat
    13801 taacaattga tgattttgcc aacagcctac atagcgcgat atactgaaaa ttctcgcagc
    13861 aactgaatgt taagcctatg aaatacgata cttccgagct ttgtgacatc tatcaagaag
    13921 atgttaacgt cgtggaaccg ctgttctcca actttggcgg acgggcgtcg tttggcggac
    13981 aaataatcac ggtaaaatgt ttcgaggaca acgggttgct gtacgatctg ctcgaacaga
    14041 atggccgtgg tcgtgttctg gtcgtcgatg gcggtggttc tgttcgtcgc gcactggtcg
    14101 atgctgaact ggcgcgtctg gcagtacaaa atgaatggga aggtctggtc atttacggcg
    14161 cggtgcgtca ggtagatgac ctggaagagt tggatatcgg catccaggcg atggcggcaa
    14221 ttccggttgg tgctgctggc gaaggtattg gcgaaagcga tgtccgcgtc aattttggcg
    14281 gtgtcacctt cttctccggc gaccatcttt atgccgacaa taccgggatt attctttcag
    14341 aagatccgtt ggatattgaa tgatagaaaa ggcaccgcaa ggtgcctttt ttctgcgtta
    14401 cctcttggcc tacacagtaa agaaattacg cgaaagatga agcgtaatca gacctcttcc
    14461 atgcgaccca gcagggcctg cagacgttcc tgccagccgt tctgctgttc tttcagatgg
    14521 ttgttctcac gctccagctc ttcgcgctga tgctgggcat tttgaacttc ctgcgacagt
    14581 gagttgtttt tttctttcag ctcttcgatt tccatctgca acagagtgat ggtatcaatc
    14641 gcctgctgta cttttgcttc cagtttctca aacacttcta atgacattgt catacctctc
    14701 ctgaattgca aggcgttgat ggataaaaat cctcgtcccg attaccggtg acgccttaat
    14761 aaatacgagc gcactttagt tagctccgat tgtatgaagc cgcgccatcg ctgtccagcg
    14821 gcacgccttg cagattacgg tttgccacac ttttcatcct tctcctggtg acataatcca
    14881 catcaatcga aaatgttaat aaatttgttg cgcgaatgat ctaacaaaca tgcatcatgt
    14941 acaatcagac ggaataaatg gcgcgataac gctcatttta tgacgaagca cacacatttt
    15001 aagttcgata tttctcgttt ttgctcgtta acgataagtt tacagcatgc ctacaagcat
    15061 cgtggaggtc cgtgactttc acgcatacaa caaacattaa ctcttcagga tccgattatg
    15121 agtcaaacat caaccttgaa aggccagtgc attgctgaat tcctcggtac cgggttgttg
    15181 atttttttcg gtgtgggttg cgttgcagca ctaaaagtcg ctggtgcgtc ttttggtcag
    15241 tgggaaatca gtgtcatttg gggactgggg gtggcaatgg ccatctacct gaccgcaggg
    15301 gtttccggcg cgcatcttaa tcccgctgtt accattgcat tgtggctgtt tgcctgtttc
    15361 gacaagcgca aagttattcc ttttatcgtt tcacaagttg ccggcgcttt ctgcgatgcg
    15421 gctttagttt acgggcttta ctacaattta tttttcgact tcgaacagac tcatcacatt
    15481 gttcgcggca gcgttgaaag tgttgatctg gctggcactt tctctactta ccctaatcct
    15541 catatcaatt ttgtgcaggc tttcgcagtt gagatggtga ttaccgctat tctgatgggg
    15601 ctgatcctgg cgttaacgga cgatggcaac ggtgtaccac gcggcccttt ggctcccttg
    15661 ctgattggtc tactgattgc ggtcattggc gcatctatgg gtccattgac gggctttgcc
    15721 atgaacccag cgcgtgactt cggtccgaaa gtctttgcct ggctggcggg ctggggcaat
    15781 gtcgccttta ccggcggcag agacattcct tacttcctgg tgccgctttt tggccctatc
    15841 gttggcgcga ttgtaggtgc atttgcctac cgcaaactga ttggtcgcca tttgccttgc
    15901 gatatctgtg ttgtggaaga aaaggagacc acaactcctt cagaacaaaa agcttcgctg
    15961 taatatgact acgggacaat taaacatgac tgaaaaaaaa tatatcgttg cgctcgacca
    16021 gggcaccacc agctcccgcg cggtcgtaat ggatcacgat gccaatatca ttagcgtgtc
    16081 gcagcgcgaa tttgagcaaa tctacccaaa accaggctgg gtagaacacg acccaatgga
    16141 aatctgggcc acccaaagct ccacgctggt agaagtgctg gcgaaagccg atatcagttc
    16201 cgatcaaatt gcagctatcg gtattacgaa ccagcgtgaa accactattg tctgggaaaa
    16261 agaaaccggc aagcctatct ataacgccat tgtctggcag tgccgtcgta ccgcagaaat
    16321 ctgcgagcat ttaaaacgtg acggtttaga agattatatc cgcagcaata ccggtctggt
    16381 gattgacccg tacttttctg gcaccaaagt gaagtggatc ctcgaccatg tggaaggctc
    16441 tcgcgagcgt gcacgtcgtg gtgaattgct gtttggtacg gttgatacgt ggcttatctg
    16501 gaaaatgact cagggccgtg tccatgtgac cgattacacc aacgcctctc gtaccatgtt
    16561 gttcaacatt cataccctgg actgggacga caaaatgctg gaggtgctgg atattccgcg
    16621 cgagatgctg ccagaagtgc gtcgttcttc cgaagtatac ggtcagacta acattggcgg
    16681 caaaggcggc acgcgtattc caatctccgg gatcgccggt gaccagcagg cggcgctgtt
    16741 tggtcagttg tgcgtgaaag aaggaatggc gaagaacacc tatggcactg gctgctttat
    16801 gctgatgaac actggcgaga aagcggtgaa atcagaaaac ggcctgctga ccaccatcgc
    16861 ctgcggcccg actggcgaag taaactatgc gctggaaggt gcggtgttta tggcgggcgc
    16921 atccattcag tggctgcgcg atgagatgaa gttgattaac gacgcgtacg attccgaata
    16981 ctttgccacc aaagtgcaaa acaccaacgg tgtgtatgtg gtgccggcat ttaccgggct
    17041 gggtgcgccg tactgggatc cgtatgcgcg cggggcgatt ttcggtctga ctcgtggggt
    17101 gaacgctaac cacattatac gcgcgacgct ggagtctatt gcttatcaga cgcgtgacgt
    17161 actggaagca atgcaggccg actccggtat tcgtctacac gcgctgcgcg tggacggcgg
    17221 tgcggtggcg aacaatttcc tgatgcagtt ccagtccgat attctcggca cgcgcgttga
    17281 acgcccggaa gtgcgcgaag tcaccgcatt gggtgcggcc tatcttgctg gtctggcggt
    17341 tggcttctgg cagaacctcg acgagctgca agagaaagcg gtgattgagc gcgagttccg
    17401 tccaggcatc gaaaccactg agcgtaatta ccgttacgca ggttggaaaa aagcggtgaa
    17461 acgcgcgatg gcatgggaag aacacgacga gtaataatga ttgcctgatg cgacgcttgc
    17521 gcgtcttatc aggcctacga gtcggataca catccggcat cacgcagact ctgaaaccgt
    17581 gcgggggcaa ccccgcacac atcaataatc cctcccttcc cctgtgctac acttcgcgcc
    17641 attccttact gcttagagtt tgctatgaga cgagaacttg ccatcgaatt ttcccgcgtc
    17701 accgaatcag cggcgctggc tggctacaaa tggttaggac gcggcgataa aaacaccgcg
    17761 gacggcgcgg cggtaaacgc catgcgtatt atgctcaacc aggtcaacat tgacggtacc
    17821 atcgtcattg gtgaaggtga aatcgacgaa gcaccgatgc tctacattgg tgaaaaagtc
    17881 ggtactggtc gcggcgacgc ggtagatatt gctgttgatc cgattgaagg cacgcgcatg
    17941 acggcgatgg gccaggctaa cgcgctggcg gtgctggcag taggcgataa aggctgtttc
    18001 ctcaacgcgc cagatatgta tatggagaag ctgatcgtcg gaccgggagc caaaggcacc
    18061 attgatctga acctgccgct ggcggataac ctgcgcaatg tagcggcggc gctcggcaaa
    18121 ccgttgagcg aactgacggt aacgattctg gctaaaccac gccacgatgc cgttatcgct
    18181 gaaatgcagc aactcggcgt acgcgtattt gctattccgg acggcgacgt tgcggcctca
    18241 attctcacct gtatgccaga cagcgaagtt gacgtgctgt acggtattgg tggcgcgccg
    18301 gaaggcgtgg tttctgcggc ggtgatccgc gcattagatg gcgacatgaa cggtcgtctg
    18361 ctggcgcgtc atgacgtcaa aggcgacaac gaagagaatc gtcgcattgg cgagcaggag
    18421 ctggcacgct gcaaagcaat gggcatcgaa gccggtaaag tattgcgtct gggcgatatg
    18481 gcgcgcagcg ataacgtcat cttctctgcc accggtatta ccaaaggcga tctgctggaa
    18541 ggcattagcc gcaaaggcaa tatcgcgact accgaaacgc tgctgatccg cggcaagtca
    18601 cgcaccattc gccgcattca gtccatccac tatctggatc gcaaagaccc ggaaatgcag
    18661 gtgcacattc tctgagtgat ttgatcgatt gagccttcca gtccttcggg actggaattt
    18721 ttttgttcgg agaacgaaga taaggcaagt caatcaaaac aggagaaaaa catggctgat
    18781 tgggtaacag gcaaagtcac taaagtgcag aactggaccg acgccctgtt tagtctcacc
    18841 gttcacgccc ccgtgcttcc gtttaccgcc gggcaattca ccaagcttgg ccttgaaatc
    18901 gacggcgaac gcgtccagcg cgcctactcc tatgttaact cgcccgataa tcccgatctg
    18961 gagttttacc tggtcaccgt ccccgatggc aaattaagcc cacgactggc ggcactgaaa
    19021 ccgggcgatg aagtgcaggt ggttagcgaa gcagctggct tctttgtgct ggatgaagta
    19081 ccagattgcg aaacgctatg gatgctggca accggtacag cgattggccc ttatttatcg
    19141 attctgcaac tgggcaaaga tttagagcgc ttcaaaaatc tggtcctggt tcacgccgca
    19201 cgttatgccg ccgacttaag ctatttgcca ctgatgcaag aactggaaaa acgctacgaa
    19261 ggcaaactgc gcattcagac ggtggtcagt cgggaaacgg cagcggggtc gctcaccgga
    19321 cggataccgg cattaattga aagtggtgaa ctggaaagcg cgattggcct gccgatgaat
    19381 aaagaaacca gccatgtgat gctgtgcggc aatccacaga tggtgcgcga tacacaacag
    19441 ttgctgaaag agacccggca gatgacgaaa catttacgtc gccgaccggg ccatatgaca
    19501 gcggagcatt actggtaagc ggttacttgt cgataaacgg tacgatcaag agatccgcac
    19561 tcaacttatt gatcatcccg cgatatgccg gcatcaaacg gttgataaat gagtggtgat
    19621 gaccacagac aaggaggtcg cactgctctt tttgtataat ttccagcagt gtttccggca
    19681 tttctccgcg ttcaattcgc agttttgtct tcggccattg aatatttttc gtcagtttat
    19741 acagcttgtt atccgactta ttcttcaaca attgaagaat atcttctgtt gcagggaagt
    19801 agatacccgg atacaactcg cttaagccat catcaatatg aattaacgtc aggtgagcgt
    19861 cattatgtct ggcgagctcc agggctttat tcaccagtaa ggcatcttct tcattcccgg
    19921 aaattgccac gccaatgtgt ttataagcca tgtttaactc cttctaaagc aactccatca
    19981 agctaacgaa cgcagtgata actcaaaaat aatcatctcg gcctggcatg agaaggtgaa
    20041 ggccgcatca agctcaactt gcccctccag atccttaatc ttataggcca cgctggcccc
    20101 ttcatctgca gaggatgcag cggcggcaag ctctttcagt ttggtcataa aggactctgc
    20161 ttcttcgcgg gtggcaaaaa agcgcgaaaa cttactggta cagttgtcgt tatcaatcac
    20221 cgtaccgata tcaatcgcac atcctttagt actgcattta tctacgacat ctttcatagg
    20281 gcacctctgt gtattcgcct ctctgtcgat tacgacttaa gaataatgcg ccgttaaggg
    20341 aagaacaaac aagtaatgat cttatggatt accatttttt ttgatattgc ttaaggtgaa
    20401 gagttcagaa ggattttccg ggcattgtca tttgcatcga ggaaataaca atcccggaaa
    20461 aaaatagcag taagagatat attaaaggtt aattacttga tttattgtcg gctttatact
    20521 tcacatcctg ggtatcttta ccatatttat tttcgccttg tgtgccaaca aacgcaccaa
    20581 gatcgataag catcatcacc agaatcaacg tcgggacaaa acgccccacc gcccattgcc
    20641 agacacccgg taaaatcgcc cagttacccg ccagcagcat ccacgccaca atcatcagaa
    20701 atgcccatgc gccggaacgc ccgcgatcat gcaagcgctt aacggttact gccgctgttg
    20761 gccagagcaa gcacacaagg caaaacgccg cggtctgaat atcgagtaaa tttttacccg
    20821 ccagtgagaa aagcaccagc atgcctgcga accacaggcc tatccaaatc cagaaatcac
    20881 ggcgtccaat acgcccttta aatgagaata accattgctg tatggtcatg taagttcctt
    20941 gatggttgtc ttttccagga ttctaccctt ttgacaaggg cgacagatat cgttttaatc
    21001 ggagccagtc ataacaaaag gtactgtcaa tgaagccagg gtgtacgctg ttttttctct
    21061 tatgttctgc attaaccgtt acaacaacgg cgcatgcaca aacaccagat acggcaacga
    21121 ccgcgcctta tctgctggct ggagccccta ctttcgatct ctccatcagc cagtttcgag
    21181 aagactttaa cagccagaat cccagcctgc cactgaacga atttcgtgcc atcgacagca
    21241 gtcccgacaa agccaatctc actcgtgcag caagtaaaat taacgagaac ttgtatgctt
    21301 ctactgcgct ggagcgcggt accttaaaaa tcaaaagcat tcaaataacc tggctaccca
    21361 tccaggggcc agagcaaaaa gccgcgaaag cgaaagctca ggcatacatg gcagcggtga
    21421 tccgcacact caccccatta atgaccaaaa ctcaaagcca gaaaaaactg cagtcgctac
    21481 taacggcggg gaaaaacaaa cgttattaca ccgagacaga aggtgcactg cgttatgttg
    21541 tcgcggacaa cggcgaaaag gggctgacct tcgctgttga accgattaag ctggcgctat
    21601 ctgaatcgct tgaaggtttg aataaatgac aaaaagcaaa gcctttgtgc cgatgaatct
    21661 ctatactgtt tcacagacct gctgccctgc ggggcggcca tcttccttta ttcgcttata
    21721 agcgtggaga attaaaatgc gacatccttt agtgatgggt aactggaaac tgaacggcag
    21781 ccgccacatg gttcacgagc tggtatctaa cctgcgtaaa gagctggcag gtgttgctgg
    21841 ctgtgcggtt gcaatcgcac caccggaaat gtacatcgat atggcgaagc gtgaagctga
    21901 aggcagccac atcatgctgg gtgcgcaaaa cgtggacctg aacctgtccg gcgcattcac
    21961 cggtgaaacc tctgctgcta tgctgaaaga catcggcgca cagtacatca tcatcggcca
    22021 ctctgaacgt cgtacttacc acaaagaatc tgacgaactg atcgcgaaaa aattcgcggt
    22081 gctgaaagag cagggactga ctccggttct gtgcatcggt gaaaccgaag cggaaaacga
    22141 agcgggcaaa actgaagaag tttgtgcacg tcagatcgac gcggtactga aaactcaggg
    22201 tgctgcggca ttcgaaggtg cggttatcgc ttacgaacct gtatgggcaa tcggtactgg
    22261 caaatctgca actccggctc aggcacaggc tgttcacaaa ttcatccgtg accacattgc
    22321 taaagttgac gctaacatcg ctgaacaagt gatcattcag tacggcggct ctgtaaacgc
    22381 gtctaacgct gcagaactgt ttgctcagcc agacatcgac ggcgcgctgg ttggcggtgc
    22441 ttctctgaaa gctgacgctt tcgccgtgat cgttaaagct gcagaagcgg ctaaacaggc
    22501 ttaagtctga caggtgccgg atttcatatc cggcacttac tttcctaaac tcctcgcctt
    22561 aacgcaaaat ctcacactga tgatcctgga tttcttcggc tgaagcacgg ttcagcgtca
    22621 gtagatttcg ttgtgtcgcc agcaatacaa atgagttatc actctgccgt accatcgcca
    22681 gcccgtagct tcccatatgt tcccgcgcct caggcacttc ttccgccagc atcataaatg
    22741 ggctgcgttg taccagttcg ctttccgtta cccgacgcgc caggtactca tgcccacgca
    22801 aaccacctgg cagtggcaac cagcggctac tgatgttcgc cagattgtta tccagttgtt
    22861 cgcgcacatc aggacgaata caagaaatat gaatatgaaa atggttttgc gtacgcccgg
    22921 tgcgggagtt gatcgccaga gataccgcgc gatcgggaac aggctggccg tatttttggc
    22981 tcataaaatc acgcgcctgc caggctaacc agaaaaaatt aggcgttgac ggatcggtca
    23041 acaatgggct ttcagtaccg ttaatacggt acgttggcat caacagatat tgcagtgggc
    23101 catgacgatc ttttaaaacc acgtatccgg cgttgggttt gacttccgca catggcgacg
    23161 ggttttggtt ttcctgctgg ttgggcaaac attgctcaag aacaatctta cgtaacgtgt
    23221 ctgactcttc accggttaat ttccagtaac caataccggc agccacaacg gcgataacta
    23281 tcatcaccaa aaaaagaaga cccgcttttt tcatcttttt ttccctgtac ctcaaagaga
    23341 tgcaagggta acgcaaaatc gtgacaaata aaaaacccag tcgcgagaaa ccaccgggct
    23401 tgataattat cctggggaaa tcagcgtttg ctgatctgat cgaacgtacc gccgttagca
    23461 aaatgctctt tttgcgcttt cgtccagccg ccgaactctt catcaatggt gaataacttc
    23521 aattttggaa acgcattttc gtactttttc gctacctcag cgtcgcgcgg acggtagtag
    23581 tttttcgctg caatttcctg accttctggc gagtagagat atttcaggta ggcttccgcc
    23641 acctctttgg tgcctttttt ctcgaccact ttatcgacca ccgacacggt tggctctgcg
    23701 aggatagact cactcggcgt aacgatttcg aatttatctt tccccagttc attcgctgcc
    23761 agcagagctt cattttccca ggcaatcagt acatcgccaa ttccgcgctc gacaaaagtg
    23821 ttagtggatc cgcgcgcgcc agaatccaga acttcgacgt ttttatacag tgcacgaaca
    23881 aaatcctgtg cttttgcctg atcgttgttg ttgtgatgca gcgcgtagcc ccaggctgcc
    23941 aggtaattcc agcgcgcgcc accagagctt ttcggattag gcgtgatcac cgaaacaccc
    24001 ggtttaatca gatcgttcca gtcatggatc tgcttcggat tacccttacg taccaggaaa
    24061 acaatggtgg aagtgtacgg tgcggagtta tccggcagac gtttgatcca ctctttatca
    24121 atccgcccgc gttcagcaat cgcgtccacg tcataggcca gagccagcgt gacaacatca
    24181 gcttcaatac cgttgattac cgacgtcgct tgtttgcctg aaccaccgtg tgactgacga
    24241 atcaccacgt tatcgccagt ttgctgtttc cagtgggcgc tgaatgcctt gttgtactgt
    24301 tcgtacaatt cgcgcgttgg atcatatgaa acgttaagaa gctgaatatc ctttgccata
    24361 acgctggttg ccgccagcaa aaatgttaac cctacgcccc actttttcat cgcccgactc
    24421 tcttatgttg tgttgtgatg agcaaagcgt gccagaaggt tgaccaaaca ttaaagaata
    24481 aaaaaagatt ggctataact tgcgagtata tgttggggga ttaaaaaggc ggaagaaatc
    24541 cgcctcatat tgctgacaaa gtgcgctttg tccatgccgg atgcggcaca aacgccttat
    24601 ccggcctaca aaagtttgca aattcaatag attacagaat tcatgtaggc ctgataagcg
    24661 aagcgcatca ggcctttttg cttctgtcat cggtttccgg ctaaaggaat ctgccttttt
    24721 ccgaaatcat taatacagtt ttttcgcgca gtccagccag tcgcctttga acggacgctt
    24781 catgttttcg atagcgtcga tgatgtcgtg gtgaaccagc tgttcgttct ggatacctac
    24841 gcaacgacca ccgtaacctg ccagcagcag ctcgatagcg taagcgccca tacgggaagc
    24901 cagaatacgg tcgtaaggca ccggagaacc accgcgctgg atgtggccca gcacagttgc
    24961 gcgggtttca cggccggttt ctttctcgat gaaatgcgcc agttcgtcaa catcacacat
    25021 atgttcggta atcgccacga tcgcgtgttt tttacctttc gcgatacccg ctttgatttc
    25081 gtttaccagg tcttcacggc tgaattcaac ttccggaacc acaacgaatt cacagccacc
    25141 ggcaatggcc gcagccaacg tcagatcgcc acaataacgg cccatcactt ccaccacgga
    25201 aatacgctgg tgagaagaag aggtgtcacg cagacggtcg atcgcttcta caacggtgct
    25261 cagcgcagtg aagaaaccga tcgtgtagtc agtgcctttg atgtcgttgt cgatagtgcc
    25321 cggcaggccg atgcacggga agcccatttc ggtcagacgc attgcaccca tgtaggaacc
    25381 gtcaccgccg ataaccacca gcgcgtcgat cccacgtttt ttcaggtttt cgatagccac
    25441 ggcgcggatg ttctcgtcgc ggaattccgg gaaacgcgca gaaccgagga acgtaccacc
    25501 acggttgatc atgtcagaaa cgctgtaacg gtctagctgt accatacggt cttcatacag
    25561 acccagatag ccgtcataaa tgcccattac ttccagacct tctgtcagcg cagaacgaac
    25621 aaccccgcga attgcggcgt tcatgcctgg cgcatcaccg ccgcttgtca acacaccgat
    25681 tttcttaatc atgactacct ctgaactttg gaatgcaaaa tgaaatctgt tgccggaagt
    25741 cttcttgcac atcgaagtga tccaacgaat gtgcaaatag tataacaatc acttcctgct
    25801 gaattgattc aggtcaggcc aaatggcggt attttataca caaaatgcgg gtctggctct
    25861 cttttatact gattatgaaa gcatagaccg tttaccctcc ctgggtacga cggaacaggg
    25921 gtcctgatgg ataatcacat ccgatcccgg aaaacgccgt aaaatagcct gctctacctg
    25981 atccgccacc atatgtgcct gaaccaaagg cagagagtct tccatttcca aatgaatctg
    26041 aataaagcgg gtcggccctg actgccgcgt gcgaagatcg tgagcgccgc taacacccgg
    26101 ccaggaagtc acgatatcaa taatttcttg ccgttcctca tcaggcaatg cgcgatccag
    26161 taatgactgt accgcctcat atcccatgcg taacgcgcta tataaaatat agatgccgat
    26221 ccccaatgca aacagagcat cggcgcgatg ccagccgtac caggacaacc ccagcgccag
    26281 cagaattgcg ccgttcatca taacatcaga ctggtaatgt agcatatcag cccgcaccgc
    26341 ctggctttgc gtccggcgca ccacccaacg ctgaaacgag acaaggataa tcgtacaaat
    26401 tagcgccaca attgtcacga taaccccgac gcctggatct gtcatcggtg ttggagatac
    26461 cagatgttga atacccgtca aaaacaggaa tagtgccgaa ccggagataa acatactttg
    26521 cgccagcgcc gcgagggact cagctttacc gtgaccaaac gagtgattat cgtcggcagg
    26581 ttgcagggaa tatcgcacca ccagtaaatt cgtcaacgac gcgccgatat ccaccagcga
    26641 atccaccagc gcggcgagaa tactcaccga cccggtatac caccatgcaa aaattttaat
    26701 cagcaatagc agcgaagcca tcgccgtcgc agcaatcgcc gcccgactga ccagccgtcc
    26761 ataagattga ttcataaata ctcccgctat caactgacgg tagtataacg gaagcaaatc
    26821 atctgcaatg cattaagcag caggcaaatt gaggataaaa aaaaccccca catcatgtgg
    26881 gggaagacag ggatggtgtc acaaaaaagc acctaacctg atgatataaa tggatttata
    26941 tcaaacactg tccacatagc gtccacatcg accataaata aagcccctca actgaggggc
    27001 tatttttgtg atcacatcca cataatttgc tgtcctgatg gtaacgggtg cggccttacg
    27061 gcgtggactt ctcccggctt cacgatgtat cgctgtaccg actcataagt gatgaacgtg
    27121 gcgctgcaat tcacgttctg acactggtga taacgctctt ttgtcgtgtc agtgatatag
    27181 cggcttgtac gcgcatgtgc ggcatgctgg cataaaggac aatgaaacat cgcgagcacc
    27241 tcttccggtt ttgttgatgg tgccatttta gttaatttat ccttataaaa caaacagata
    27301 aaataaaaac atcactcatc atcttctgtt tcgtactcca catcagaaag cctgacctca
    27361 agctctaagg acgtcgtgaa gccgctatta ttcagaaaat gtgtcacctt agtgattgtc
    27421 cagtcctgct cgtctatgac acgcttaaag cctgacactt taaccggtgt ttccgtgtaa
    27481 atatctgccc gaccggtagc caggctgatg gagaactccg ccacaccccg ttgcagttta
    27541 tcccacttcg cctgagcggc gcgcatggcc tgtgctttcg tggcatatac cgtggtcagt
    27601 gcaaaaacgt tgtcagcctc accggccatg tattcacctt cgcgcgcttc cggtactttt
    27661 ggcgctttct tctgcgtgac cggtttcgct ttcgggtgct ccagtgcgcg caggtgtttc
    27721 tctttctttt tgcgtttcag ttttaccttc tgcttttgcg gcttcgggtc tttggtgtgt
    27781 aaccactttg ccgttacgcc ggtataggct ccacggtcag caatcgcaaa atgatggcgg
    27841 tcgccgtcgc tgcgggttat ggtgacctgc gggacttttt taccgctggc cgtcaccccc
    27901 tgccccgctt tgagaaacaa cagttttccc atttttaccg acacctcacc gccgttgcgt
    27961 tcagcaaggc gggtcaggaa ttttgcatca gactcctgcg actggtcgat gtgcgggatt
    28021 ttaattccgg ccagtgacgg agcgacactg gcttccagcc tgttacggga ggctatcgcc
    28081 tcaacaatcg caccgagcgt ggtgtcatgc caggagcctt cacggcggga attgagcgtc
    28141 ccgcgaaaat ctgcactccg ggcgcggatg gtgaccacat ccggcgcgcc ccggtgttca
    28201 acctcatcaa cggtaaattt ccctttgcat accagggcaa aacctttcca gccgatatac
    28261 accgtcagga cagcgccacg aaccggcagc ccgacctgcc cgtcggcatc gttcagttca
    28321 atatcaagct ggtcagcctc aaagccccgg ttatccgtca gggtcatgct catcagacgg
    28381 tcgctgatat tgccggtaat atccctgctg tcgagcatca gcatgtaatc cggcgtcagc
    28441 gtactgcctg catcaaatgt cagtgcatcc agcattatcc cgcccccgtc atacccgtga
    28501 atttagtcgc catactgcca gccttaccga tgagcgattc cgcctgttta ccgatatcgc
    28561 cataaagcgc ggccagtgat tcatcaacgc gggtgagcga cagcgtaaaa tcaatttttc
    28621 gtggtgtgcc gtctgcaaag aaaatactcc ctgtttcact caccctgctg atgacataca
    28681 tgccgtaaat catgccggtg ccatccagca acggccacgc ccgaccttcc tctgccatca
    28741 gcctgagcgt agtcatcgtc agctttccgc cggtcagttc aggataaagc acaccggcaa
    28801 gcgtgatgtt ttcatcgcca acaccgagaa actgaaaggc atcccgttta ccgatacggg
    28861 aatttgacgg ccagcgataa tctgattcac gctgcatggt ctggtgtggc agcgtctggc
    28921 gcataaaaac aaacatacct aacgcgagca tcatttttcg tcacctcctt aaccgtcatg
    28981 catcatgctg gcacgggcgc gcgcacgttt atcccgctcg tatttttcga gcgcatcctg
    29041 taactggcgg tcgagctgtg tccccggcgc agtaccaccc gtcaggctga tgtgatattc
    29101 gtttttactc tggtctacat aagagcggcc agccggtgcc gtgaccggct gataagcctg
    29161 ataacctgca taagagctgg tcgccggaat ataaccaccg ctgccatacg tggcggcttg
    29221 agttctggcg gcggtctggt caagtgtgtc tgactctttg ttgatgaccc cgagcttttc
    29281 cagtacccag tcaatgccgc tgcgcagttt gttgaacgca ttaagcggca gcatcagcgc
    29341 gtcagccagt gcctgcccga acatgacgcc cgtgtcacgg caacggttca gggtgtcctg
    29401 tgtggctttg accggggcaa tcaggttttt aaaccactgc cacgcggcct gtaacttttc
    29461 gcccagccag tcaaacaccg gcttaagtgg cgtgaacagt tcccccaccg gcgcaaatgc
    29521 cgctttcagc ccttccacca caccgccaaa gaatgcgctg acaggctccc agtatttacg
    29581 gataagcaac gccccggcga caatggcggc caccacggcc acaaccggcc agctaatcgc
    29641 cccgatggcc gtcataacgg cactgccaac cgtcgtgaag attgcaccca ttgcgcctgc
    29701 tgccgcgatg atggcattga tgccggtgat aaccggccag gcgacaagac caattgcacc
    29761 gatgatgcca gtaagcgcca gtgcgccacc ggcaatgagg ccgatggttg acgccagtga
    29821 tttgtttttc tggatccagc catcgagttt taacacatac tttgtggccg tctgtgtgag
    29881 attacgcagt gcgccttcct gctggtcaaa caggtcagtc cccaccgcct cataagcaga
    29941 ctgaaactcc ttaaagtcac cgccgaggtt atcctgcatg attttaacca gctcttccgt
    30001 tttaccgtcc gaggctttca gcgtggcggt cagcttatcc agttttccgc ttgctgccgc
    30061 tgccagtaaa acgttcgctg atttcagggc ttcctcacca aaaatggttt taaggtattc
    30121 ccccttctga gacgttccca gcttgtgttt atcaaagctg gcctgaatct ctttcagaat
    30181 ggtgaacaac ggacgcatat ttcccttttt gtccgaggtt ttaacgccaa gctctttgag
    30241 tgcatcccat gcttttccag tcggtgcctg taatcgggtg acaacggcac tgctccccgt
    30301 ccccgccatt gaccccctga tgttattgtc atgcagcaca cctgtcatgg ccgctgcctg
    30361 ttcaagactt acacctgccg tcctagcaac cggaccgagg taagtcagtg catcactgag
    30421 tccctgaaaa tcagccgccg acttattcat cgttgccgac aacacgtcgc ccacatggct
    30481 gacatcatca tttgacagtt gaaaggatgc cttagtcccc agcaacagtt gcgcgttttc
    30541 ttccatcgac cgctgattcg ccagtgccat attcagtgtg accggcgttg ccgcctgaat
    30601 agccgcagca tctccacccg ctttcgcaat gataatctgc gcaccggccg catcatccgc
    30661 cgaggcggcg gtattatcgc cgagctggcg cgcctgcttg cgtagtgcag ccatttcggc
    30721 ggagtctttt gccacaccga gcacggcctg caattctgag tttttctgcg caaactcata
    30781 accgggcatc agcaacttaa ctccggccat cgttcccgcc gccgcaatcc ccacaccggc
    30841 agcgcccact gaggccatat ttccggccag ttccttgcct gcctgataac gctgttttac
    30901 tgcgttaagt tttgcctgtt gcgcactgac acgcgccagc gcgtcacgct gacggttaag
    30961 ctgtgcggtg gtttcactga tacggttttt cagtccctgc tcatcacgtg caagattgcg
    31021 ggtattaatt cccacaacgg ccagttcccg ctgctggcgt ttaacggaat ccgtcaggcg
    31081 gttatatttc gcctgtaagt cctccgccgc acgcttcgcg gactccagca ctttcgcctg
    31141 agcacgtgtc ggacgttcgg tgtttttaaa ctgtgtggca agggcttcgg cctcctgccg
    31201 tgccttttca agtgcatgac cagtcacggc gagctgtgcg ctggtcttgc ggaatccctc
    31261 aatacgggat gcgtgaccgt tcagctcgcg cagtgatttt tgtgtttccc ggatatcccc
    31321 cgacagcgac ctgctcgctg tgcggatgga tttaaacggg cgggatgcct ggtcaacagc
    31381 tctgagcaat acctgtaatt ttacattgtt actcattcgt atttccgctt cgccggagcg
    31441 ccttttcgcg ccatgtgatg agttcggtca ggctcatggg atacagttct gatggcggcc
    31501 agtgaaatat cactgccaca tccgccatca ggtcatcgac cgacagattt ttcggaaacg
    31561 tcactgcacc gagttcggag acaaaaaacc gaccacctta ccggccagcg ccacaaggtc
    31621 aggcagttcc agcgcggcga cttcctgctc ggtcagcatc ggtgccgtca tgcgcggcag
    31681 caccttaatc agtgcatcga cttcggagtt cgcgaccgct gccagactga caccgcgcag
    31741 cgtcccggca ttaggtttca tcagcgtgac ctgttcgata acctgctcac cacgtttaac
    31801 cggattgtcc agggtaatga cattttcttt gttcatggtt ttctcacttc tgaatcaggg
    31861 ttaaccggtc agcctggctg accggatgaa aatcacaggc cgatatttcg gcggtgttgc
    31921 tccagccggt cgacgccgtt caccttctca atcatgttaa tggtgtcgat ttcgaccagc
    31981 tccttaccgt ccatcgtcag ccggaaatag gtgcagacca cggagatttt cgactcggtg
    32041 tcttctccct gtttcccctc gccggtgtcg atttctttct gacgtccacg catgaccacc
    32101 tcgacggcca ccgtttcgcc ggtatcgtcg cgctggtaag aaccggcaaa acgaatcggc
    32161 acggcatcca caccggttgc ggcgtaaagc tcccagataa ccgaatccgg gaagcccccg
    32221 agagaccact ccattgacag cgcatcgtca tcaaggccga ggtctaccgg tgcgctgccg
    32281 ttcattcccg caccgcgata gttttcgagc ttacgggtca gttttggcag cgtgacggac
    32341 tttgcaacgc cctgatagct gtagccgttc agaaagacgt tcattaactt gagtttgcgc
    32401 ggcattgcca tcagtcaggc tccttaattg ctgttaaccg aggtgaccag atttgccagg
    32461 tatttatcgg taatacgctg gcgcagggtc aggttttcaa gaggaggcac cggggtatag
    32521 tcgtagtcga tatacagttt tccggcctta agggtttccg catcgttgga ttcttcgctg
    32581 aaccagcagg tcgcatccac gatatagccg tttgttttca gctcacgaaa tttggcattg
    32641 atgccgtcaa cgatgtcgtg aatcagcgtt gcggtgatgg gcttgtccac cgcccacatg
    32701 tgcgcctcag ccatcgtgtc ggccagcacc tgcgcggtgc gggtgtagtt ttcaaagagg
    32761 aacagcgggt catcggagca ggtacggtta ccccagaagc ggaaaccgtc gcggcgaatc
    32821 agcgtagtga cgcctgactc gttaagcagg tcagcatcgg tgccggactc ctgcaaatcc
    32881 cagaagacgg atgcgctgat gccggtaaca ccgttcaccc cgacattgga cagcgtttta
    32941 tgccagccct gctcctggtc gattttagcg cgcagaccca gcgcacgggc ggtggcatac
    33001 gcggtggcgg tggtactggt gaccgtatcc catgcgagga aatccggcca gatgaccatc
    33061 agctcacgct ggctgaaatt ctgacggtag gctttcacct cggaaatagt tttacagccc
    33121 catgcgctga tatacccgaa agcgcgcagc ttctgacaga ctgatgccag tgcaacagcc
    33181 acctctttgg tatccagtcc cggcacgccg agaatacgcg gtttaacacc ggttaccgac
    33241 tccgccgcca gcagggcttt cagcccggtg tactgaccgt tttcgtcggt ggtgccgatg
    33301 atattggaaa cggtctgcgc gagtttcgtt tcttcgtcgt cgccggtgcc gtcttccacg
    33361 cgcacgacaa cggtgaccgg ttttgactgg tcggcgatag cctgcaacga tgccgccagc
    33421 gtgccttttt taccggcctt tgcaatcgcg ctctgcacat tggtaatcag caccggttta
    33481 ttgaggggaa aggtttccgc atccgcatcg ctggccgtgc agaccatgcc gacaatggca
    33541 gtggatacgg tggaaatgac gcgggtgccg tcgttaatct ccagcacctg cacgccgtga
    33601 tgatagtcac tcatccgttt aactccgtgg ttaatgggtg agtggtattt tctgttgtgc
    33661 agagcatgag acgctatttg acctggctgg tcagtggatg aaacaacaga taaagaaaag
    33721 gcggacaatc cgcccgcctg tcatgatttg tacacactca ttttccgact gacaatttac
    33781 ataacccaaa agttatcaaa tctgacagtc tgctttgagc aagaagcgga catttgcctg
    33841 tcagggtata tgccgctctg cggttattat ccagaatcaa taattcatct gagttg
//
LOCUS       DAZFJL010000035        33611 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFJL010000035 DAZFJL010000000
VERSION     DAZFJL010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.035"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            64..348
                     /gene="pduA"
                     /locus_tag="ACHJ8C_001698"
     CDS             64..348
                     /gene="pduA"
                     /locus_tag="ACHJ8C_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFO5551565.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAARNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            345..1157
                     /gene="pduB"
                     /locus_tag="ACHJ8C_001699"
     CDS             345..1157
                     /gene="pduB"
                     /locus_tag="ACHJ8C_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HFO5551566.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQGTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDRPSYI"
     gene            1176..2840
                     /gene="pduC"
                     /locus_tag="ACHJ8C_001700"
     CDS             1176..2840
                     /gene="pduC"
                     /locus_tag="ACHJ8C_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFO5551567.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPANQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNIKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            2850..3518
                     /locus_tag="ACHJ8C_001701"
     CDS             2850..3518
                     /locus_tag="ACHJ8C_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFO5551568.1"
                     /translation="MEINEKLLRQIIEDVLAEMQTSDKSVSFRAPVSATVSSVPDTEN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            3533..4051
                     /gene="pduE"
                     /locus_tag="ACHJ8C_001702"
     CDS             3533..4051
                     /gene="pduE"
                     /locus_tag="ACHJ8C_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFO5551569.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPSASIQSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTAQDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLERRYQAQICAAFVREAAE
                     LYVERKKLKGDD"
     gene            4061..5893
                     /locus_tag="ACHJ8C_001703"
     CDS             4061..5893
                     /locus_tag="ACHJ8C_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFO5551570.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSTLAETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGLTITPQELLTCPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGSNIGGMLEHVRQTMAELTNKPSHEIFIQDLLA
                     IDTSVPVNVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQKGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            5883..6233
                     /locus_tag="ACHJ8C_001704"
     CDS             5883..6233
                     /locus_tag="ACHJ8C_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFO5551571.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDQEKLIVHYKNLPASAPLFTLMYQQDNHARRSIGTNA
                     ARLVKGIPFRELHS"
     gene            6253..6528
                     /gene="pduJ"
                     /locus_tag="ACHJ8C_001705"
     CDS             6253..6528
                     /gene="pduJ"
                     /locus_tag="ACHJ8C_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFO5551572.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            6552..6974
                     /locus_tag="ACHJ8C_001706"
     CDS             6552..6974
                     /locus_tag="ACHJ8C_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFO5551573.1"
                     /translation="MKQSLGLLEVSGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSETP
                     ATTVISEIISCNLCLDPACQRQKGEPRSLCLHANKRGE"
     gene            6977..7609
                     /locus_tag="ACHJ8C_001707"
     CDS             6977..7609
                     /locus_tag="ACHJ8C_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFO5551574.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSTADYLRLFPE
                     QPISEKKLLMQPGQFAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IKGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            7606..8097
                     /gene="pduM"
                     /locus_tag="ACHJ8C_001708"
     CDS             7606..8097
                     /gene="pduM"
                     /locus_tag="ACHJ8C_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFO5551575.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQEIVHQY
                     ASLQIRYVDLPLLRQLAGNETSDRAAIQIHEALAWGLHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHRDRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            8101..8376
                     /gene="pduN"
                     /locus_tag="ACHJ8C_001709"
     CDS             8101..8376
                     /gene="pduN"
                     /locus_tag="ACHJ8C_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HFO5551576.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            8388..9395
                     /gene="pduO"
                     /locus_tag="ACHJ8C_001710"
     CDS             8388..9395
                     /gene="pduO"
                     /locus_tag="ACHJ8C_001710"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFO5551577.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQLRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETIVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPGA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            9392..10777
                     /gene="pduP"
                     /locus_tag="ACHJ8C_001711"
     CDS             9392..10777
                     /gene="pduP"
                     /locus_tag="ACHJ8C_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
                     /protein_id="HFO5551578.1"
                     /translation="MNNSELETLIRNILREQLVPATPRMQRNAIFQTVDEAVCAAHQA
                     FLRFQQCPLKTRSAIISAIREELMPRLTELAEESASETGMGNKEDKYLKNKAALNNTP
                     GIEDLTTTALTGDGGMVLFEYSPFGVIGSVTPSTNPTETIINNSISMLAAGNSVYFSP
                     HPGAKYVSLKLITTIEDIAFRCSGIHNLIVTVSEPTFAATEQMMAHPKVSVLAITGGP
                     GIVAMGMKSGKKVIGAGAGNPPCIVDETADIVKAAEDIINGASFDYNLPCIAEKSLIV
                     VASVADYLIQQMQSFGALLLNHEQTEKLRAICLPDGSANKKLVGKSPSALLEAAGLPL
                     PAKAPRLLIAVVDADDSWVTCEQLMPMLPIVKVNDFDSALALALKVEDGLHHTAIMHS
                     QNVSRLNLAARVMQTSIFVKNGPSWAGIGVGGEGFTTFTIATPTGEGTTSARTFARSR
                     RCVLTSGFSIR"
     gene            10788..11900
                     /locus_tag="ACHJ8C_001712"
     CDS             10788..11900
                     /locus_tag="ACHJ8C_001712"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFO5551579.1"
                     /translation="MKTFSLQTRLYSGTGSLNILSRFTNRHIWIICDVFLAHSPLIDT
                     LHQALPDSNRLSIFSEITPDPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGTGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGIAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRAAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLKTTCTLPTLTNALKEKKAEWS
                     VRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            11897..13240
                     /locus_tag="ACHJ8C_001713"
     CDS             11897..13240
                     /locus_tag="ACHJ8C_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO5551580.1"
                     /translation="MNVELTSCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARVDTFL
                     VNAAECEPMLKVDQQLMVQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVSVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTINGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGSLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLAVAKTVCEQCRLCTDLCPRHLVG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLTPWYQDAPLSEQVPQTEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            13243..13797
                     /gene="pduT"
                     /locus_tag="ACHJ8C_001714"
     CDS             13243..13797
                     /gene="pduT"
                     /locus_tag="ACHJ8C_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFO5551581.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSVASERAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            13797..14147
                     /gene="pduU"
                     /locus_tag="ACHJ8C_001715"
     CDS             13797..14147
                     /gene="pduU"
                     /locus_tag="ACHJ8C_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFO5551582.1"
                     /translation="MTTQHTAERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTVS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            14152..14595
                     /locus_tag="ACHJ8C_001716"
     CDS             14152..14595
                     /locus_tag="ACHJ8C_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO5551583.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     SMEQISLVKCWLREAGAHNVLVTSAVNNNGVTELFALLHTEEGCR"
     gene            complement(14696..15025)
                     /gene="tmaR"
                     /locus_tag="ACHJ8C_001717"
     CDS             complement(14696..15025)
                     /gene="tmaR"
                     /locus_tag="ACHJ8C_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO5551584.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(15197..16255)
                     /gene="yeeA"
                     /locus_tag="ACHJ8C_001718"
     CDS             complement(15197..16255)
                     /gene="yeeA"
                     /locus_tag="ACHJ8C_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO5551585.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLDNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(16453..16926)
                     /gene="sbmC"
                     /locus_tag="ACHJ8C_001719"
     CDS             complement(16453..16926)
                     /gene="sbmC"
                     /locus_tag="ACHJ8C_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO5551586.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(17045..18211)
                     /gene="dacD"
                     /locus_tag="ACHJ8C_001720"
     CDS             complement(17045..18211)
                     /gene="dacD"
                     /locus_tag="ACHJ8C_001720"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO5551587.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVIMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            18420..19847
                     /gene="sbcB"
                     /locus_tag="ACHJ8C_001721"
     CDS             18420..19847
                     /gene="sbcB"
                     /locus_tag="ACHJ8C_001721"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO5551588.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTTWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSENVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(19921..21279)
                     /gene="plaP"
                     /locus_tag="ACHJ8C_001722"
     CDS             complement(19921..21279)
                     /gene="plaP"
                     /locus_tag="ACHJ8C_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO5551589.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSLPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(21269..21331)
                     /gene="yoeI"
                     /locus_tag="ACHJ8C_001723"
     CDS             complement(21269..21331)
                     /gene="yoeI"
                     /locus_tag="ACHJ8C_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO5551590.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(21546..22475)
                     /gene="yeeY"
                     /locus_tag="ACHJ8C_001724"
     CDS             complement(21546..22475)
                     /gene="yeeY"
                     /locus_tag="ACHJ8C_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5551591.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(22521..23345)
                     /gene="yeeZ"
                     /locus_tag="ACHJ8C_001725"
     CDS             complement(22521..23345)
                     /gene="yeeZ"
                     /locus_tag="ACHJ8C_001725"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5551592.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(23428..23682)
                     /gene="yoeB"
                     /locus_tag="ACHJ8C_001726"
     CDS             complement(23428..23682)
                     /gene="yoeB"
                     /locus_tag="ACHJ8C_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFO5551593.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(23679..23930)
                     /gene="yefM"
                     /locus_tag="ACHJ8C_001727"
     CDS             complement(23679..23930)
                     /gene="yefM"
                     /locus_tag="ACHJ8C_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFO5551594.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLIDSIDSLKSGKGTEKDIIE"
     gene            24213..24263
                     /gene="hisL"
                     /locus_tag="ACHJ8C_001728"
     CDS             24213..24263
                     /gene="hisL"
                     /locus_tag="ACHJ8C_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO5551595.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    24239..24362
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            24409..25308
                     /gene="hisG"
                     /locus_tag="ACHJ8C_001729"
     CDS             24409..25308
                     /gene="hisG"
                     /locus_tag="ACHJ8C_001729"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO5551596.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPIDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            25314..26618
                     /gene="hisD"
                     /locus_tag="ACHJ8C_001730"
     CDS             25314..26618
                     /gene="hisD"
                     /locus_tag="ACHJ8C_001730"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO5551597.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCNKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALSTSRLIVTKDLAQCVTISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            26615..27685
                     /gene="hisC"
                     /locus_tag="ACHJ8C_001731"
     CDS             26615..27685
                     /gene="hisC"
                     /locus_tag="ACHJ8C_001731"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO5551598.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKVVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            27685..28752
                     /gene="hisB"
                     /locus_tag="ACHJ8C_001732"
     CDS             27685..28752
                     /gene="hisB"
                     /locus_tag="ACHJ8C_001732"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO5551599.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLRLQ
                     KAGYKLVMITNQDGLGTQSFPQADFEGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREALNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            28752..29342
                     /gene="hisH"
                     /locus_tag="ACHJ8C_001733"
     CDS             28752..29342
                     /gene="hisH"
                     /locus_tag="ACHJ8C_001733"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO5551600.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            29342..30079
                     /gene="hisA"
                     /locus_tag="ACHJ8C_001734"
     CDS             29342..30079
                     /gene="hisA"
                     /locus_tag="ACHJ8C_001734"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO5551601.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVATLLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            30061..30837
                     /gene="hisF"
                     /locus_tag="ACHJ8C_001735"
     CDS             30061..30837
                     /gene="hisF"
                     /locus_tag="ACHJ8C_001735"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO5551602.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            30831..31442
                     /gene="hisIE"
                     /locus_tag="ACHJ8C_001736"
     CDS             30831..31442
                     /gene="hisIE"
                     /locus_tag="ACHJ8C_001736"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO5551603.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLHQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVNDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(31628..32605)
                     /gene="wzzB"
                     /locus_tag="ACHJ8C_001737"
     CDS             complement(31628..32605)
                     /gene="wzzB"
                     /locus_tag="ACHJ8C_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO5551604.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGPAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGKTPEGAQKQLAKYIQQVDDQVN
                     EELEQDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKADDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(32752..>33611)
                     /locus_tag="ACHJ8C_001738"
     CDS             complement(32752..>33611)
                     /locus_tag="ACHJ8C_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFO5551605.1"
                     /translation="VVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGK
                     ALYDNLHPSRIVIGERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLA
                     MRVAYFNELDSYAESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLA
                     NYQSVPNNLISAIVDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGI
                     MKRIKAKGVEVIIYEPVMKEDTFFNSRLERDLATFKQQADVIISNRMAEELKDVADKV
                     YTRDLFGSD"
BASE COUNT         8530 a         8718 c         8490 g         7873 t
ORIGIN      
        1 gccattcttt ttcctcgctt ttttttatag tcatatcgtc aaaaaaatcc aggcgaggtt
       61 tttatgcaac aagaagcgtt aggaatggta gaaaccaaag gcttaactgc cgccatagag
      121 gccgcggacg ccatggtgaa gtcggcaaat gttttgctgg tcggctacga aaaaataggt
      181 tccgggctgg tgaccgtcat cgttcgtggt gatgtcgggg cggtaaaagc ggcaacagac
      241 gcgggtgccg cagctgcgcg taacgtcggc gaagtaaaag ccgtacacgt tattccccgc
      301 ccacataccg atgtcgaaaa aatcttacca aagggaatta acccatgagc agcaatgaac
      361 tggtcgaaca gatcatggcg caggttatcg ctcgcgtagc gacgccagag catgaggttc
      421 tcccgaaaac cactcatcaa atacgagaga cggctatggc agaaaaaagc tgcacgttaa
      481 cagaatttgt tggcaccgct attggcgaca ctgtaggtct ggtcattgcg aacgttgata
      541 gcgccctgtt ggacgcgatg aaacttgaaa aacgctatcg ttccattggc attctggggg
      601 cgcgtattgg cgcagggcca cacattatgg ccgctgatga agcggtaaaa gccaccaaca
      661 ctgaagttat tagcattgaa cttcctcgcg atactaaagg cggtgccgga cacggctcat
      721 tgatcctctt aggtggcaac gatgtttccg acgtgaaacg cggtattgaa gttgccctga
      781 aagaactcga tcggaccttt ggtgatgtct atgccaacga agccgggcat cttgaactgc
      841 aatacaccgc ccgcgccagc tatgcactgg aaaaagcgtt tggcgctcca ctgggacggg
      901 cttgcggcgt tattgtcggc gctccggctg cggtgggtgt tctgatggcc gacaccgcgc
      961 tgaagtccgc caatgtggat gtcgtggcat acagctcccc cgctcaaggc accagctaca
     1021 gcaacgaagt cattctgatt atctcgggtg actccggcgc agtccgccag gcggtcatct
     1081 ccgcacgcga aatcggtaaa accgtgcttt cgactctcgg cgatacaccg aaaaacgatc
     1141 gcccttccta catctgaatt ctctgaggca gatttatgag atcgaaaaga tttgaagcac
     1201 tggcaaaacg cccggtcaac caggacggtt tcgttaaaga gtggattgaa gaaggcttca
     1261 ttgcgatgga aagcccgaac gatcctaaac cgtcaatcaa aattgttaac ggcgtcgtta
     1321 ccgaactgga tggaaaaccc gccaatcagt ttgacctgat cgaccacttt atcgcccgct
     1381 acggtatcaa tctcgcgcgc gcagaagaag tcatcgcgat ggattcggta aaacttgcca
     1441 atatgttgtg cgatccaaat gtcaaacgta gcgatatcgt tccccttacc acagcaatga
     1501 cgcctgcaaa aatcgtcgaa gtggtttcgc agatgaacgt ggtagagatg atgatggcga
     1561 tgcagaaaat gcgcgcccgt cgaacaccat cacaacaggc tcacgtcacc aacatcaaag
     1621 ataacccggt acaaattgcc gccgacgcag ctgaaggcgc atggcgcggg tttgatgaac
     1681 aggaaaccac cgttgccgtt gctcgctatg caccgttcaa cgccatcgcc ctgctggtcg
     1741 gctcacaggt gggacgtccg ggcgtcttga cccagtgttc actggaagaa gccaccgaac
     1801 tgaaactcgg tatgttaggc cacacctgtt acgccgaaac aatttctgtc tatggtacag
     1861 aacctgtttt caccgatggc gatgataccc cgtggtcaaa aggatttctc gcgtcatcct
     1921 atgcttctcg cggcttaaaa atgcgcttta cttccggctc cggctctgaa gtacagatgg
     1981 gttacgcgga aggcaaatca atgctttatc tcgaggctcg atgcatttac atcaccaaag
     2041 ccgcaggcgt tcagggtctg cagaatggtt cggtaagctg tatcggtgtt ccatcagctg
     2101 tgccatcagg catccgcgca gtattagcgg aaaacttaat ttgctcatca ctggatctgg
     2161 aatgcgcctc cagtaatgac cagaccttta cccactcgga tatgcgccgt accgcacgtt
     2221 tcctgatgca atttctgcca ggtaccgact ttatctcttc cggtttttcc gctgtgccga
     2281 actacgacaa catgtttgct ggttcaaacg aagatgctga agacttcgat gattacaacg
     2341 tcattcagcg tgacctgaag gttgatggcg ggctgcgacc agtacgcgaa gaagatgtta
     2401 tcgcaatccg caataaagcc gctcgcgctt tacaggctgt ctttgccggt atgggactac
     2461 cgccaattac cgatgaagaa gtcgaagcgg caacctatgc ccacggttcg aaagatatgc
     2521 ctgagcgcaa tattgtggaa gacatcaagt tcgcccagga gatcatcaat aaaaaccgta
     2581 acagcctgga agtggtgaaa gccctggcgc aaggcggttt taccgatgtc gcccaggaca
     2641 tgctcaacat gcaaaaagca aaactgaccg gcgattatct ccatacctca gccatcatcg
     2701 tcgatgacgg acaagtgctc tctgcggtca atgacgtcaa tgattacgcc ggaccagcta
     2761 caggttaccg cctgcaaggg gaacgctggg aagagatcaa aaatattccc ggtgcacttg
     2821 atcccaacga aattgactaa gggtgagaaa tggaaattaa cgaaaaatta ctgcgtcaga
     2881 ttatcgaaga cgtgctggca gaaatgcaaa ccagcgacaa gtccgtttca tttcgcgcac
     2941 ctgtctctgc aactgtctct tcagtgccag ataccgaaaa ttttctgacc gaaattggcg
     3001 aagcccaaca aggcactcag caagatgaag tcattattgc tgtaggcccg gcgttcggcc
     3061 tggcgcaaac cgtcaatatc atcggtattc cgcataaaaa cattttgcgc gaagtgattg
     3121 ccggtatcga agaagaaggc attaaagccc gtgtgattcg ctgctttaaa tcgtccgatg
     3181 tcgcattcgt agcagtagaa ggagatcgtc ttagtggttc aggtattgct atcggtattc
     3241 agtcgaaagg tacaacggtt atccaccagc aaggtcttcc ccccttgtct aacctggaac
     3301 ttttcccaca agcgcctttg ttgacgctgg atacttatcg tcagattggc aaaaacgccg
     3361 cgcgttatgc gaagcgcgag tctccacaac cggtgccgac cctcaacgat caaatggcgc
     3421 ggcctaaata ccaggccaaa tcagccattt tgcatatcaa agagacgaag tacgtggtca
     3481 ctggcaaaaa accgcaagaa ctgcgcgtga cgttttaaca aaggaaacct tcatgaatac
     3541 cgatgcaatt gagtcgatgg tcagggacgt gttgaaccgc atgaacagtt tacaggatgc
     3601 agctcctgtt tctgcagtcc ccagcgcgtc aatacagagc gccaaagtga cggattaccc
     3661 gctggcaaat aaacatccgg aatgggttaa aacagcaacc aataaaacgc tcgacgattt
     3721 cactctggaa aatgtgctca gcgataacgt caccgcccag gatatgcgca ttacccctga
     3781 aacattgcgt attcaggccg cgattgccag agatgctggt cgtgatcgcc tggcgatgaa
     3841 tttcgaacgc gccgctgagc ttacctcagt accggatgat cgcatcctcg aaatctacaa
     3901 tgcgttacgc ccttatcgtt cgactaagca agaacttatt gccattgctg acgatcttga
     3961 acggcgttat caggcccaaa tttgtgctgc atttgtccgt gaagctgcgg aactttatgt
     4021 cgagcgcaaa aaactgaaag gcgacgatta aggacgaact atgcgatata tagcaggcat
     4081 tgatatcggc aactcatcaa cagaggttgc gctggcaacg ctcagtgcaa ctggagagtt
     4141 gtcgttcgtg agtagcaccc tggcggaaac caccggaatt aaaggtacgt tgcgtaacgt
     4201 ccatggtatt caggaggcgc tcgcacaggc aacgaaaaaa gtcggcatca atgttagcga
     4261 tatttcgctt attcgtatca acgaagcaac tccggtcatt ggtgatgtgg cgatggaaac
     4321 catcactgaa actatcatca ccgaatcaac catgatcggt cacaacccga aaactccggg
     4381 cggagtgggg ctgggcgtag gactcaccat cactccgcaa gagttattaa catgcccggc
     4441 ggacacaccc tacattctgg tggtgtcatc ggcatttgat tttgccgata tcgcaacgat
     4501 gataaacgcc tccgtacgag ccggatatca gctgactggc gttattctgc aacgcgacga
     4561 tggcgtactg gtcaataacc gcctggaaat tcctctccct attgttgatg aagtcctcta
     4621 catcgatcgt attccgctcg gcatgctggc tgcaattgaa gttgccgttc ccgggaaagt
     4681 tatcgaaacc ctgtctaacc cgtatgggat tgccaccgtt ttcgccctca atgctgaaga
     4741 gactaaaaat attgttcctg tcgcgcgggc gctaatcggc aaccgttcag cagtggtagt
     4801 gaaaacgcct tctggcgacg ttaaagcccg ttcgataccc gccggaaata tcgaactgct
     4861 ttctgccggg cgcacaacgc gggttgatgt agctgctggt gccgatgcca ttatgaaagc
     4921 ggtcggtgag tgcccaaaac tggaaaacgt gacgggagaa cctggctcca acatcggcgg
     4981 aatgctggaa catgttcgcc agactatggc tgaactgacc aacaaaccca gccacgaaat
     5041 ttttattcag gatttgctgg cgatagatac ctctgtcccc gtcaacgtaa ccggtggtct
     5101 ggcaggtgaa ttttcactgg aacaggctgt gggtattgcc tctatggtga aatccgaccg
     5161 tctgcaaatg gcgatgatcg ccagtgaaat taagcaaaaa ctgcatgttg atgttcaggt
     5221 cggaggcgca gaagcagaag ccgccattca gggggccttg actacccctg gaacgacacg
     5281 tccactggca attctggact taggtgcagg ctctaccgat gcctccatca tcaatcagaa
     5341 aggggaaata gttgccactc accttgcggg agcgggcgac atggtaacga tgattatcgc
     5401 tcgtgagctg ggcttaaatg atcgttatct ggcagaagaa atcaaaaagt acccgttggc
     5461 aaaagtagaa agcctgttcc acttacgcca tgaagatggc agcgttcagt tcttccctac
     5521 tcccctatcc cctcatgtat ttgcccgcgt ttgtgtggtg aaacctgacg aactggtgcc
     5581 tattcccggt gatttgacgc tggaaaaagt acgcgctgtc cgtcgcagtg cgaaagagcg
     5641 tgtcttcgta actaatgccc tgcgtgcgct gcgtcaggta agccctgccg gaaatattcg
     5701 cgacattccg ttcgtcgttc tggtaggtgg ctcttcgctg gatttcgaag taccgcaatt
     5761 ggtcaccgac gcgctggcac attatcgact ggtcgccggg cgggggaata tccgtggtag
     5821 cgaaggtccg cgcaatgcgg tagctactgg gctcctcatc gcctggcaca aggagtcgat
     5881 tcatggaaag taaagagcgt gcaccagcca tcgttgtcat ggagatcggt gactgtatca
     5941 ctacatggga tgaagtgctt cttggcattg aagaggaagg tattcccttt cgcattcaac
     6001 acattccgtc aggtgaagtt atcgatagcg cctggctggc ggctcgtcaa tctcccctgt
     6061 tggttggcat tgcctgcgat caggaaaaac tgattgtgca ttacaaaaac ttaccggcat
     6121 cagcgccact gtttacgctg atgtatcaac aagataacca tgccaggcgc agcatcggca
     6181 ctaacgctgc ccgcctggta aaagggattc ccttccggga actccactca taatccacag
     6241 gagaaacgca gtatgaacaa cgcactagga ctggttgaaa caaaagggtt agttggcgca
     6301 attgaagctg cagatgccat ggttaaatcc gccaacgtgc agctggttgg ctatgaaaag
     6361 attggttcgg gtctgattac ggtgatggtc cgtggcgatg ttggcgcagt aaaagccgcc
     6421 gttgatgcag gaagcgccgc cgcaagcgcc gttggcgaag tgaaatcctg ccatgtcatc
     6481 ccgcgcccac acagtgatgt tgaggctatt ttacctaaat cagcataacc ccattcgcta
     6541 aggagcacag cgtgaagcaa tcactgggat tacttgaagt cagtggtctg gcactcgcca
     6601 tcagttgcgc agatgctatg gcaaaagccg cgtccatcac gctggttggc cttgagaaga
     6661 ccaatggctc aggctggacg ctcatcaaaa tcaccggtga cgtcgcctcg gttaatgcgg
     6721 cgatcgcgac gggagccagt ttcgccggac agcgaaatgg cctcgtcgca cacaaagtga
     6781 ttgcccgtcc gggagacggc attctgcccc acacaactga agcattgact gtgacggttg
     6841 aaacaagcga aacaccagca acaaccgtca tctcagagat tatcagttgt aatttgtgtc
     6901 ttgacccggc atgtcagcgg caaaaaggtg agccccgctc actttgcctg cacgccaata
     6961 agcgagggga gtaacagtgg ataaacaaac cttgcaggaa acagtgagcc agattctgga
     7021 tgagatgcgc gagcgcccta tccctctggg tgtttccagt cgtcatattc atctttcaac
     7081 agcggattat ttgcgtcttt ttcctgaaca accgattagc gagaaaaagc tgttaatgca
     7141 acctggccaa ttcgctgctg aacaggttgt tacgctggag ggaccaaaag gtaaattgca
     7201 caacgttcgc ttgttggggc cgctacgcaa tgcaagccag gtggaaattt cccgtacgga
     7261 cgccagaacg ttagggatca ccgccccgct acgcatgtca ggcgatctca gcggcactcc
     7321 agggattcgt ttgataagcc catttgcgga actggagatc acatctggcg tcattgtcgc
     7381 acaacggcat attcatatgt ctccgctgga tgccctgatt tatggcgtta cgcatggcga
     7441 taaagtggcg gttgccatta aaggtgatga gcgacgactg atattcgaaa acgtcgctgt
     7501 tcgcgtctct ccggatatgc gtcttgaaat gcatattgat accgatgaag ccaacgccgc
     7561 aggggctgat agccctgacg tttttgccac actggtgtca gtgccatgaa taacatgctg
     7621 atgcaacgta ttgttgacga agtggttttt cgccttaaac aacgtgcggg aaaaacactc
     7681 gtgctgactg tttttcaact gcgagacgcc agcgttcaag agatagttca tcagtatgca
     7741 tcattacaaa tccgatatgt cgatctgccg ctgctgcgac aacttgcggg gaacgaaacg
     7801 tcagatcgag cagcaattca gatacacgaa gcgttagcct ggggattgca cattcaattg
     7861 tctttgcagc gtcatttcct gaacgccatt gaattgaaga ctctcgcccg tctaccgcta
     7921 agctggtgcg acgagcaagg gcaaccgatc taccttcatc gtgatcgact tttaagttat
     7981 gccgatattg cacagttaag ctcaggaata ctggtgttac agcgaaaatg ttgtgtgacc
     8041 gcactggctc gtgaagcggc aattacccga aatattcagt taatcaggca ggagtaacct
     8101 atgcatctgg ctcgagtcac tggcgcggta gtatccacgc aaaaatcacc ttctctgatc
     8161 gggaaaaaac tgctgctggt tcgccgggtc agtgctgacg gcgaactgcc tctcacgcca
     8221 ttaaccggcg acgaagttgc cgttgattca gtcggcgcag gcgttggtga gttggtactc
     8281 ctcagcagtg gttccagcgc cagacatgtg ttttccggcc ctaacgaagc catcgacctg
     8341 gcagtggtcg gcattgtcga tacgctttca cgctaaggag ttgaaagatg gcaatttata
     8401 cccgaacagg tgattcagga agtacgtctt tatttaccgg acagcgcgtc agcaaaactc
     8461 atctacgtgt cgaaacctat ggcacgctgg atgaactgaa cgcgacactc agtctgtgtt
     8521 attgcgccac cgccatcgaa agccatcgca tcttactgga agccattcaa caacagatat
     8581 tttggtttag cgctgaactc gccagtgaaa gcgaacagcc gtcagcgcag ctgcgttaca
     8641 tcggtacgga agaaattgcc gcgctggaaa acgcgatcga tagcgcaatg aacgccgtcc
     8701 ctccagttca cagcttcatt ctgcctggtc gatgtgaagc cgcaagccgc atgcatttcg
     8761 cccgcacggt ggctcgacgg gctgaaagac gtctggttga actgacaacg gaaaccatcg
     8821 tccggaatgt tttgctgcac tatatcaacc gactttccga ttgcctgtat gccctcgccc
     8881 gcgtggagga caatgttgct catcaaaacc tgatgattca ggaaatcaca aaacgttatc
     8941 acgaagccaa ccacacacca gcattgaagg aacgcacaat gccgctcact tttcaggatc
     9001 ttcaccagct cattcgtagt gcggcaatgc gcgcagatga actgcatatt cccgttgtca
     9061 tcagcattgt tgatgccaac ggaacagaaa gtgttacctg gcggatgcct gatgccctgt
     9121 tggtcagcag tgaactggca ccgaaaaaag cctggaccgc cgtggcgatg aaaacggcaa
     9181 cccacaaact tgctgatacc gtgcaacccg gcgcgccgct ttacgggctc gaaagccata
     9241 tgcaaggcaa agtcgtcact tttggtggcg gcttccccct ctggcgtgac ggaaaattgc
     9301 ttggcgggct tggcatcagc ggcggtagcg ttgaacaaga catggatatt gcccaaagcg
     9361 caatggcggc aattaacgtg ggagtaaacc aatgaataac tctgaattag aaacactgat
     9421 ccgcaatatt ctgcgtgaac aacttgtccc cgctacgcca cgaatgcaaa gaaacgctat
     9481 ttttcagact gttgatgaag cggtctgcgc agcacaccag gcctttttac gctttcaaca
     9541 gtgcccgctt aaaacccgta gcgccattat cagcgccatt cgcgaagaat taatgccccg
     9601 ccttacagaa ctggcagaag aaagcgccag tgaaacaggg atgggaaata aagaggataa
     9661 gtacctaaag aacaaagcag cactcaataa tacgccagga attgaagatt taaccaccac
     9721 tgcactaacc ggtgatggtg gcatggtttt gtttgaatac tccccgtttg gggttattgg
     9781 ctcggtcacg ccaagcacca accccacgga aaccatcatt aacaacagca tcagcatgct
     9841 ggcagcaggt aatagcgtct acttcagtcc acacccggga gccaaatacg tttcgttaaa
     9901 actgattacc acaatcgaag acatcgcgtt tcgctgtagt ggcattcaca acctgatcgt
     9961 taccgtatct gaaccaacct ttgccgccac cgaacagatg atggcgcacc ctaaagtcag
    10021 cgttctggcg attaccggcg gcccggggat cgtcgccatg ggaatgaaaa gtggcaagaa
    10081 agtgattggt gccggagcgg gtaacccacc ctgcattgtc gatgaaacgg cggatattgt
    10141 gaaagccgcc gaggacatca tcaacggcgc ttcatttgat tacaacctac cgtgtatcgc
    10201 tgaaaaaagc ctgattgtcg tggcgtcagt ggcagattat ttaattcaac agatgcaaag
    10261 ctttggcgca cttcttctta accatgaaca aacggagaaa ttgcgtgcaa tttgcctgcc
    10321 agacggtagc gccaacaaaa aactggttgg gaaaagccca tcagccttgc tggaagcagc
    10381 gggcctccct ttgcccgcaa aagcgccgcg cctactgatt gctgttgttg acgccgatga
    10441 tagttgggtt acgtgtgaac aactgatgcc aatgctgccc attgtgaagg tcaacgattt
    10501 tgacagcgct ctcgccctgg cgttgaaagt ggaagacggc ttgcaccaca cggcaattat
    10561 gcactcgcag aacgtctcac ggctcaacct tgccgcgcgc gttatgcaaa cctcaatctt
    10621 tgtcaaaaac ggcccctcct gggcgggtat tggcgtcggc ggcgaaggct ttacgacctt
    10681 taccattgca acaccgaccg gagagggtac aacgtcagca cggacttttg cccgctcacg
    10741 acgctgcgtg ttaaccagcg gcttttccat tcgctagaga aacggaaatg aaaacttttt
    10801 cactgcagac gcgtttatac agcggaacgg gaagtctcaa catactgagc cgtttcacga
    10861 ataggcacat ctggattatt tgcgacgtct ttctggcaca ctcaccactc attgacacgt
    10921 tgcatcaggc gctgcccgac agtaatcgtc tcagtatttt tagcgaaata acgcccgacc
    10981 caactatcca gaccgtggtg aaaggcattg cgcaaatgca gactttacgc ccggatgtgg
    11041 ttatcggttt tggcggcggt tctgccctgg acgccgcaaa agcgattgtc tggtttggtc
    11101 gccagtgtgg cattgaaatt gaaacctgcg tagccattcc taccaccagc ggtaccggtt
    11161 ccgaagtgac cagtgcttgt gtcatcagcg atcctgagaa agggattaaa tatccccttt
    11221 tcgataatgc actttatccg gatatcgcga tccttgatcc atctttggtc gtcagcgtgc
    11281 caccggcgat aacggccaat accgggatgg atgttttaac ccatgcgctt gaagcttatg
    11341 tttctccccg cgccagcgac ttcacggatg cactggtcga aaaagccgtg caaattgttt
    11401 ttcaatacct gcccactgca gtgaagaaag gcgattgtct tgccacccgt ggcaaaatgc
    11461 ataatgcatc gacgcttgca ggaatagcct ttagccaggc cggactcggt ttaaaccatg
    11521 caattgctca tcaactcggc ggacagtttc atctgccgca cggactggcg aatgcgctcc
    11581 tgctcacggc ggttatccgc tttaatgctg gcgatccgcg cgcagccaaa cgctacgccc
    11641 ggctggcgaa aacgtgccat ttatgcccgg acaacgccaa tgacaccgcc agcctgaatg
    11701 cacttattca acatattgaa cagcttaaaa caacctgtac gctgccgact cttaccaatg
    11761 cgctgaaaga aaaaaaagca gaatggtcag ttcgcatacc agatatggtt caggcggcac
    11821 tggcggatgc aacgttgcgt accaacccgc gtgcggctga tgcctccgcg attgcagaac
    11881 tgctcgagga gttgttatga acgtcgagtt aacctcctgc gacgaccaga ccatccgcga
    11941 gcgagtacaa gcagcaggtg tcgtgggtgc tggcggcgca ggttttccaa cccatattaa
    12001 gctacaagcg cgtgttgaca cgttcctggt gaatgcggcg gaatgcgaac caatgttgaa
    12061 agtggatcaa cagttaatgg tgcagcaagc cagccgcctg cttcgcggtg ttcactatgc
    12121 tatgaaagcc accggggcca gtagcggaat catcgcgctg aaagaaaaat atcaacgggc
    12181 aataaacgca ctcacaccac tgttaccacc ggatatccgc ctgcatattt tgcccgatgt
    12241 ttaccccgca ggagacgaag tgctgaccat ctggatggca actggtcgcc gcgttccacc
    12301 cgctgcatta cccgttagcg tcggtgtggt ggtcaataat gttcaaaccg tacttaatat
    12361 tgccagagcc gtcgagcaac agtatccagt gacgcgccgc acactcacta ttaatggtgc
    12421 agtggcgaaa ccaataacgg taaccgtccc catcggcatg tctttacgtg aagtcctggc
    12481 gctggcgggc ggcgcaacag tcgatgcgcc tggttttatc aatggcgggc cgatgatggg
    12541 aagtttgatc acatcgctcg acacccccgt cagcaaaacc actggcggat tgctggtttt
    12601 acccaactct cattcactga tccaacgtcg aatgcagaat gaccgcagcg tacttgccgt
    12661 cgcgaaaact gtttgtgagc aatgccgctt gtgtaccgat ctctgcccac gtcatttagt
    12721 cggtcacgaa ctggcacccc atttattagt acgtgcggtt aactaccaac agctcgctac
    12781 gccacaactg ttactgacgg cgctcacctg ttcggagtgc aacgtctgcg ccagcgtcgc
    12841 ctgtccggta ggaatttcac caatgcgcat aaaccgcctg cttaagcagg aattacgcgc
    12901 acaaaatctc cgctacgaag gtgccttaaa ccctgctgat cccatggcaa attatcgact
    12961 gattcccgta aaacggctgg taaccagact tggcttaacc ccgtggtatc aggatgcgcc
    13021 attgtctgaa caggtgcctc agaccgaaaa agtgacgtta ttgttacgcc agcacatcgg
    13081 tgccagcgca attgcgtgtg tacaaaaagg cgatcgcgtc gttcatgggc aatgtgtagg
    13141 tcaaattcca cacggcacat taggtgcccc gattcatgcc agtattgatg gcatggtcag
    13201 cgatgtgaca gaaaacgcaa ttacgcttgt gagaggttaa cgatgtcgaa agcggtaggt
    13261 attttagaac ttagcagtat cgcgaaaggg atggaaaccg gcgacgccat gctgaaaagc
    13321 gcaaacgttg agttactggt cagcaaaacg ctatgtcccg gaaaatttct gctgatgctc
    13381 ggtggcgatg tgggcgcggt gcaacaggcc atcaacagcg gagcgtccct tgctggtgaa
    13441 atgctggtcg acagtcttgt cctgccgaat attcatccca gcctcctttc tgcaatcagc
    13501 ggacttaacc atgttgaaca gcaccgggct gtcggcgtag tcgaaacctg gagtgtcgcc
    13561 gcctgcattg acgctgccga ttgtgctgtc aaagctgcga atgtcattct cgttcgcgta
    13621 catatggctt ttggcattgg tggtaaatgc tacatggtgg ttgctggcga tatttcagat
    13681 gttaataacg cggtctctgt cgccagcgag cgtgcggggg aaaaaggttt gttggtctat
    13741 cggtcagtga ttgcccgtcc acatgaagcc atgtggcgtc agatggtgga gggataatga
    13801 caactcagca cacagctgaa cggatgattc aggaatatgt ccctggtaag caggtcacgc
    13861 tggcacatgt catcgccaat cctgggaagg atctgtttaa gaaattgggt ttaccagaca
    13921 ccgtttctgc tattggcatt ctgacgatta cccccagcga agcatctatc atcgcatgtg
    13981 atatcgccac aaaatcaggc gctgtagaga tcggttttct tgatcgtttt accggtgccg
    14041 tagtgctgac tggtgatgtt tctgccattg aatatgcact tagccaggtt acccgtacgc
    14101 tgggtgaaat gatgcgcttt accgcctgcc ccattacgcg gacctgacgt tatgcaacgt
    14161 atgatgctta tcggccccag ccagtgcgga aaaacgtcac tcacgcaatg tctgcgtggc
    14221 gaaaatatcc attatcagaa aacgcaggca atcgtctggt ccccgaccac cattgataca
    14281 ccaggcgaat acctcgaaaa ccgcagcctc tacagtgcat tgttggtcag tgcctgcgaa
    14341 gcagacatta tcgcgctggt acttaatgcg gacgcaccgt ggtcaccttt ttcgccaggc
    14401 tttacctcgt caatgaatcg cccggtcatt ggagtagtaa ccaaagcaga tttagccagc
    14461 atggaacaaa tatctttggt gaaatgttgg ttacgggaag ctggagcgca caacgtgtta
    14521 gtaaccagtg cggttaataa caatggagtt acagaacttt ttgccttgct gcatacagaa
    14581 gaaggctgcc gctaatttcg ataggttctc agatgagaat gatgacggtg atacacagat
    14641 aagaatcaat gagcaatgag ggttgccggg caaccctcat tgaataaaac aggaattact
    14701 tcgcttcgcc gtttttcatt tcgcccatag ctttcagctt tttggagata tcgcggcgtt
    14761 ctttggagag ctcggcattt ttgatgatgt aatcgtcaac gcgatcttca tagtcacctt
    14821 tcatgctggc gatgatgccc tggattgctt caacgctcat ccctggcttg atgtaatcgc
    14881 tcaggttgtc cagcaacagg acgcgcttct ggttgtcacg gatctttttc tcaacgtcct
    14941 gaatttcacg ttgcagtttg ttcttacgac ggaacagacg aacaaattcc agtacgtcct
    15001 ggaatgaagg cttggtagtt tccattttta tacccctgat aatgtgagag tcggattcgt
    15061 ttaagcaacc gctattcgtt agggccaaca ttactcatag ctgccgcaga tgacaatgct
    15121 tttatccctt ttcactatca taccctttat ccttgctgaa tcgaagcagc agcaagatga
    15181 ttctgaagtt caggaattat ttgcgcaagg cccggcaaat cagattcgat agcaactcaa
    15241 gctgatgtgc cgtttccatg ttcagccaca cataaccata gattggtgtt tccacgactt
    15301 tcaggtcatg gtggttatcg agcaactgac gcagctcttc cacagcatcg ttcaattttt
    15361 ccgtattggc aaaaaccggc tgcggattac cttcgtacag cgcatgaaca aggctcagca
    15421 gtatttgctg catcatgtgc tgggtatcac gaagtttttg cgcgttcaat aacacgaaat
    15481 ggctggggcg cgtggcccag tatgcattga tttgcaactc cagcatacaa accagattgc
    15541 gattaatggt ctggatacct tcatatatcg atttgggaat acgggtttct ttgctggcgg
    15601 gcgcaatcag cccacgcatt ttcacggcat cggtcagcag tttttgtaga tggctttcca
    15661 gacgcgggcg ttcgagtaag ttcggtgaga atgcagattg atagacccga ttatactcgg
    15721 tcagactttt tgccagttga atgcgccagt ggatgaacgc ccgttgtggc cagataccgg
    15781 taaacaacat tgccagtaaa gagccgagaa tcacatcgcc gcttcgccat aacgccgtat
    15841 caatttcacc tgtcggggaa cccacaacaa ttgccagcgt caccccaatc aataaacctt
    15901 gatatggttt cttgcccagc gccagccaac cgcaaaggaa catggccgcc gcgcaccaga
    15961 ctaacatcag cggtaacgag attaactcca gttgcagagc gataagacct aaaatcgacc
    16021 ccaacaccgt accgccaata cgttcgaagg cacgagggac aacgttcccc cagaacgaga
    16081 ttggccccat aatcaccacc atggtgacca gcggccaggt gctttccggg atagtaaaca
    16141 ggcggataat gagaaaagtg agcaggaatg ccagcgcgac ccgagtacca tgcacaatgc
    16201 ggtaatggcg gtatacccgg atttcaaacg ggcttaatga cttatcggca cgcacgcaga
    16261 aactccgtaa tgaagattaa aagcgctcat aactcatctg tcggccagaa ggcgcgcctc
    16321 tgaattatcc gacggacaaa ccgggaacag tgtaatttcc cggcaggagc gaaaaaattg
    16381 aaaggcgcat cttactcttt tcgctttcaa aaaaagagtg gtcttcgcgt caacacaccg
    16441 ccctgagatg aattagtgat gttttggctg caccgcaaca tacatttcga tatcccagta
    16501 cccatcttcc gcgccattgt tcaagtaaac ctcgaagcag ggctttggta acatttcata
    16561 agcactgtcc tgcaagaggc tattaaagaa ctgataccag ggtttagcaa aatcatcacc
    16621 gactacacga gccaccgcca ccgcatactg accacctgaa atttctgtca gaatgacgcc
    16681 ctcactgttt tcggggagcg taaagttatt cggcaccgtc acgacggtgt cgcagcgtaa
    16741 tttttcggcg ggtgtttcat ctggattgtc gtaatagaca gcaacccact ccttcggcac
    16801 aatatttttg ctatctaccc acatcatcaa ctgctcaaag cctttcttta ccgtctgttc
    16861 ccacgggcca acgagatgga aacctgcaac ggtacgttta tcttcctgct taatctcgta
    16921 gttcatgacg cctccattaa cactgttttt atatacagta taattacaat attaaaacca
    16981 caaggaatga gtgttgatta tgcgagcaga ctcgcactcc tgccagtctg ctgcaaaaga
    17041 aaggtcaggc cttatggtgg aaataatcac tcaggcgaga aaacatgctg ccttccccga
    17101 cagattccag ggtaaccagc ggccagtgcg ccacctgttt atcacggtcg taaagttcaa
    17161 tttcccctac ccgctgatgg gcgctaattg gcgcggtgag ctctttacca tcaagggtat
    17221 atttggcttt gatatgtgga atttcggctt tcggtagcac catccagaac tcttgttccg
    17281 ttcccagggc gatattttct ttatcaccat accagatgcg ttccgtacca acctttttcc
    17341 cacggtgcaa aatttgcacc gtagtaaagt tttgttgacc ccaacgcagt aattttcttg
    17401 cctcttcctc acgacctttt gcgctgtcag cacccattat cactgcaatg agacgacgct
    17461 gcccatccac agccgaagca atgagattaa acccggcacc agaagtatga cccgttttca
    17521 ggccgtcaac attcatggtt ttatcccaca ataacccgtt acggttttgc tgggtaatac
    17581 cgttccaggt gaggcttttc tcactataca tatgataaaa ctcgggctcg ccgtggatga
    17641 tagcgcgaga aagcacagct aaatcataag ccgagctatg ctggccaggt gcatccagac
    17701 catgcactgt ttcaaaatgc gtatccttga gatgcagctt ctcggcatag ttgttcatca
    17761 tttcaacaaa ctgccgttgc ccaccggcaa tatagtcagc cagtgcaaca caagcgtcat
    17821 ttccggaatc cacaattaaa ccacggctta aatcacgtac cgatacgcga tcgccctctt
    17881 tcaaaaacat cagtgaagaa ccgacaaaca ctggattatc tttcgcccac gcatcgcgcc
    17941 ctacggtgac aatatcgtct ggcgtaatgc gatgactatc gatagcgcga tccacgacat
    18001 aacccgtcat cagctttgtc aggctggcgg gattgcgctg ttgatgctca ttacccgcgg
    18061 tgaggatctg tccggtggtg taatccatca atacccagga cccggcatga atctctggag
    18121 gctgaggtga aaaaggaatg ttttccgccg caaaaccaga cgataagtta aaaacgaaca
    18181 aagaagcagc aataataaga cggcgtttca acagcaaacc ctcaggagtt tcaaatagct
    18241 gttcttttta cggaaatact tatgaactgg ctggaataaa gtgcaagaaa atgtgactac
    18301 cctcgcattt ttatctgaca tgatctgttg ccactcgctg tcaaattgtt gcgctaaagc
    18361 tgattagcac ggtgatattt gatactctgg cagacagcag aaacaacgga tttaacctaa
    18421 tgatgaatga cggtaagcaa caatctacct ttttgtttca cgattacgaa acctttggca
    18481 cgcaccccgc gttagatcgc cctgcacagt tcgcagccat tcgcaccgat agcgaattca
    18541 atgtcatcgg cgaacccgaa gtcttttact gcaagcccgc ggatgactat ttaccccagc
    18601 ctggagcagt attaattacc ggtattaccc cgcaggaagc acgggcgaaa ggagaaaacg
    18661 aagccgcgtt tgccgcccgt attcactcgc tttttaccgt accgaagacc tgtattctgg
    18721 gctacaacaa tgtgcgtttc gacgacgaag tcacacgcaa cgttttttat cgtaatttct
    18781 acgatcctta cgcctggagc tggcagcatg ataactcgcg ctgggattta ctggatgtta
    18841 tgcgtgcctg ttatgccctg cgcccggaag gaataaactg gcctgaaaat gatgacggtc
    18901 taccgagctt tcgccttgag catttaacca aagcgaatgg tattgaacat agcaacgccc
    18961 acgatgcgat ggctgatgtg tacgccacta ttgcgatggc gaaactggta aaaacgcgtc
    19021 agccacgcct gtttgattat ctctttaccc atcgtaataa acacaaactg atggcgttga
    19081 ttgatgttcc gcagatgaaa cccctggttc acgtttccgg aatgtttggg gcatggcgcg
    19141 gcaataccac ctgggtggca ccgctggcgt ggcatcctga aaatcgcaat gccgtaatta
    19201 tggtggattt ggcaggagac atttcgccat tactggagct ggatagcgac acattgcgcg
    19261 agcgtttata taccgcaaaa gccgatcttg gcgataacgc cgccgttccg gttaagctgg
    19321 tgcatatcaa taaatgtccg gtactggccc aggcgaatac gctacgcccg gaagatgccg
    19381 accgactggg aattaatcgt cagcattgcc tcgataacct aaaaattctg cgtgaaaatc
    19441 cgcaagtgcg tgaaaaagtg gtggcgatat tcgcggaagc cgaaccgttt acgccttcag
    19501 agaacgtgga tgcacagctt tataatggct ttttcagtga cgccgatcgt gcagcaatga
    19561 aaattgtgct ggaaaccgag ccgcgtaatt taccggcact ggatatcact tttgttgata
    19621 aacgaattga aaaactgttg ttcaattatc gagcgcgcaa cttcccgggg acgctggatt
    19681 atgccgagca gcaacgctgg ctggagcacc gccgccaggt cttcacgcca gagtttttgc
    19741 agggctacgc tgatgaattg cagatgctgg cacaacagta tgccgatgac aaagagaaag
    19801 tggcgctgtt aaaagcgctt tggcagtacg cggaagagat cgtctaaaac aaaaaagccg
    19861 gagcgacaaa ctccggcttt ttgagattta ccggatgatt atgtcatccg gcttgtaaga
    19921 ttatgcaacg tcttcgtact gcggaaccgg gttacggaag ctcttggtga cgcaagccag
    19981 gtaaatcaga ccgatagcag cccagatcag acccagaacc attgagcttt cttccaggtt
    20041 aacccacagc gcaccaacgg tcagcgcacc acacatcggc aggaacagat actggaagtg
    20101 atctttcagc gtcttgttac gcttctcacg gatccagaac tgcgagatga ccgacaggtt
    20161 aacgaaggtg aacgccacca acgcaccaaa gttaatcagc gccgtagcca ttaccaggtc
    20221 gaagttgatt gccagcaggg caatcgcgcc aaccaggatg atgttcatcg ccggagtacg
    20281 ccatttcggg tgtacataac cgaagaagct tttcgggaac acgccgtcac gacccattac
    20341 gtacatcaga cgcgccacgc ctgcgtgcgc cgccataccg gacgccagta cggtaatggt
    20401 ggagaagatc agcgcgccaa cctggaacgc tttacccgca acgtacagca tgatttcagg
    20461 ctgtgacgca tccggatctt taaagcgaga gatatccggg aagtacagct gcaggaagta
    20521 agttgcaaag atgaagatca tgccgccgat cagcgcggtc aggaaaatcg cacgcgggat
    20581 cacgcgctct gcatctttgg tttcttccga caggttgctg atgccgtcaa aaccggtaaa
    20641 ggagaagcac aggattgtcg ccccggtaat catcgggata acatgcgcat caccagacca
    20701 gaacggacga gtgctcgcca gcgtaccagc gccttcacct tcaaatacgc cataaacaac
    20761 catgcccaga atcaccgcga tcagcactac ctgcaacacg acgattacag tgttgaagtt
    20821 cgctacggat ttcagactac gcaggttaaa ggcggtcata aaggccacca gcgccaccac
    20881 aaacatccac gatggaaggg aaggcaccag agcttcaaaa tagattttcg ccagcagaat
    20941 gttgatcatc ggcgcgaaca gatagtcgag cagagaagac caacccacca taaagccgac
    21001 agtcgggcta atggatttct gggcgtaagt gtatgcagag ccagcagaag gatagcggcg
    21061 aaccagcttc ccgtagctca gagccgtaaa caggatcgca atcaatgcga acgcataggc
    21121 tgtcggcaca tgaccatccg tgaggcctga aacgatacca aatgtatcaa acagcgtcat
    21181 cggctgcata taggctagac ccatcattac aaccggaact aacgtaagcg ttttacgcaa
    21241 ttccacgcga gaggtgtttg gagtaacgtt atgcgacatg gtcattctcc tttacggtga
    21301 taaccgtcgc gtaagcaaaa aattgcccca tttttttgga ttcctcagcg acaacaactg
    21361 tcgattttta gtaaatatct atccggtacg aagcccggcc tcttggtatg aatgattggt
    21421 ttgaagcaaa aaataaccga cgctaattaa acgtcggttt ttagtcattt tttgacagcg
    21481 gcgcattgtg cctaaatggg ggggaaatga caagagaatg agaggcttgt cataataatt
    21541 tttctttaaa tggctgattt tccgtcatca gattttgcat aaacaccggc gatcgcacta
    21601 tttgctaaaa tttcatctcg ccaccagccg ctggcaagcc ctgcggtctg ttcattccag
    21661 ccaatccaga caggttcaaa gagagtttgg gcgaccactt tcttctcgat taacgcgcca
    21721 ctatcgagaa aacgttgcgc cagataccgt ggtaaatagc cgcagccaag gccgctaatt
    21781 tgcagctcca gcttggtttt aaaatcgaaa acggtaatgg cttcttgttc gtcgagaagt
    21841 tgcgaggccg tagaggcgcc tgcctgcgca gtgtccccca ccacaatcgc ccgatatcgt
    21901 ttaatgatac gacggtttaa cggttcttct tccagggcta acggatgatg aggcgcgacc
    21961 gcaaagactt gttcaagatc gcccaaccgc gagaaaccaa actcactgga agatggtggc
    22021 tcatgcatcg cgccaacgat aatatccgct cgcccctgag tcagggcatc ccaggaacca
    22081 ccgagtacgc cattgataaa cttcaggcgc gtcacgctat gatgttgata aaaagcttca
    22141 atgagaggcg caagcagaga aaaaggaaac gtgtcgtcca cgccaatcac cagctcgttt
    22201 tcccagcctt catgcagttt gatcgcctgc ttttccagtt ctcgcacggt atgcagaact
    22261 tcccgccctt tctctaataa catttttccg gtacgggtga atttagcgcg gtgaccgcta
    22321 cgatcaagca attgaatatt aaggtcgctt tccagcttgt gaacggtgta actcagagcg
    22381 gacggcgtct tgtagagttt ggctgacgcc gccgcaaaac tgccttcttt ttctaaggca
    22441 tcgaggatca tcaacacatc cagcagtggt ttcatacacg cccccttgca gtgcatgcgg
    22501 cgtcctgctg gcgtgatcgt ttactccagc ggcattacca gcggatcggg atactggtat
    22561 tcaaatccca gttcattaca aatccgactg ccatcaataa tcttaccttt gccgctgtcc
    22621 agactatttc tgaactgcgg aggttccagc cccagtaaac gggccatctg cggatagaaa
    22681 acattacgcg cagggtgagc tggcgcacat atattataga tgtgtccgcc tttaggtgcc
    22741 tgtaacaaca gagtgatagc gccaataaca tcttctaaat ggactaaatt aacaccatgt
    22801 tcaccatcag gggcggtttt tccggcaaag aagcgtccgg gatgacgtcc cggtccgacc
    22861 agtcccgcaa gacgcaggat atcgaccgaa gtaccaggta aattgtgcag ccagtcttcg
    22921 agttcttcta atactcgtcc actgttggtt accggattac gcggggtggt ttctttcacc
    22981 gtgccttgcg cgtcgccata gacagatgtt gagctggtaa aaataatgcg gggaatacga
    23041 tgggccagcg cgctatccac taactcttgt accgcttgta aatagaactc atcgccgggg
    23101 ccgctacgac gtgccggaag cgtaatgacc agcgcatcgg catccatcag ggcatccaga
    23161 tcgtcagaat cgcaaactaa ctcaggctcc atgcgaagca gatagctatc aatgccactc
    23221 attcgggccg cttcgacacc atcttgtgtg gttttactcc cggtgacttg ccagcctcgc
    23281 gctgaaagtg acatcgccag cggcatgcct aaccacccta acccgacaat tgcgaccttt
    23341 ttcatccgtt atctcctgat acaccttaga tctataaggc tacgctagcg tatcaaaact
    23401 gacaattcat tctatgaatg aatctgttca ataatgataa cgacatgctg caatgagcag
    23461 tgaatcatca gtaaccgcgt ataccagacg gtgctcctct gtaatgcgtc gggaccagaa
    23521 acctgacaaa ttatgtttca ggggttctgg tttcccctta ccttcaaatg gtgttctgcg
    23581 ggtatctttg ataagttcat tgatcttttt aacaattcgc ttatctgttt cctgccagta
    23641 cagataatca tcccatgatt cctcagacca gattagtttc actcaataat gtccttttcc
    23701 gttcctttgc ctgatttcag gctatcgatt gagtcaatca atctccgggc gttagcgggg
    23761 gaacgcagta gataagccgt ctcttccagc gagttgtatt cttcgagtga catcagaaca
    23821 caagcctctc cattctgacg agtaatgagg atcggggcat gatcttcaac ggctttcatc
    23881 attgttgccg acaaattctg acgcgcttcg ctgtagctaa ttgtacgcat gtaaatctcc
    23941 tcttttgtac agttcattgt acaatgatgg gtgttaatta actatttatt aattagtttg
    24001 tagatcaaga cattgtcagc gagacgaaaa atcagacttt gctctttttg gtgctgtcaa
    24061 gttagaggac agtcctctta gccccctcct ttccccgctc attcattaaa caaatccatc
    24121 gccataaaat atataaaaaa ccccttgctt tctaacgtga aagtggttta ggttaaaaga
    24181 catcagttga ataaacattc acagagactt ttatgacacg cgttcaattt aaacaccacc
    24241 atcatcacca tcatcctgac tagtctttca ggcgatgtgt gctggaagac attcagatct
    24301 tccagtggtg catgaacgca tgagaaagcc cccggaagat caccttccgg gggctttttt
    24361 attgcgcggt tgttaacggt tcagacaggt ttaaagagga ataagaaaat gacagacaac
    24421 actcgtttac gcatagctat gcagaaatcc ggccgtttaa gtgatgactc acgcgaatta
    24481 ctggcgcgct gtggcattaa aatcaatctt cacacccagc gcctgatcgc gatggcagaa
    24541 aacatgccga ttgatattct gcgcgtgcgt gacgatgaca ttcccggtct ggtaatggac
    24601 ggcgtggtag accttgggat tatcggcgaa aacgtgctgg aagaagagct gcttaaccgc
    24661 cgcgcccagg gtgaagatcc gcgctacttt accctgcgtc gtctggattt cggcggctgc
    24721 cgactttcac tggcaacgcc gattgatgaa gcctgggatg gcccgctctc cttaaatggt
    24781 aaacgtatcg ccacctctta tcctcacctg ctcaagcgtt atctcgacca gaaaggtatc
    24841 tcttttaaat cctgcttact gaacggttct gtcgaagttg cgccgcgtgc cggactggcg
    24901 gatgcgattt gcgatctggt ttccaccggt gccacgctgg aagctaacgg cctgcgcgaa
    24961 gtcgaagtta tctaccgctc gaaagcctgc ctgatccagc gcgatggcga aatggaagaa
    25021 tccaaacagc aactgatcga caaactgctg acccgtattc agggtgttat ccaggcgcgc
    25081 gaatcaaaat acatcatgat gcacgcaccg accgaacgcc tggatgaagt catcgccctg
    25141 ctgccaggtg ccgaacgccc aactattcta ccgctggcgg gtgatcaaca gcgcgtagcg
    25201 atgcacatgg tcagtagcga aaccctgttc tgggaaacga tggaaaaact gaaagcgctg
    25261 ggtgccagtt caatcctggt cctgccgatt gagaagatga tggagtgatc gccatgagct
    25321 ttaacacaat cattgactgg aatagctgta ctgcggagca acaacgccag ctgttaatgc
    25381 gcccggcgat ctccgcttct gaaagcatta cccgcaccgt taacgatatt ctcgataacg
    25441 tgaaaacgcg tggcgatgaa gccctgcggg aatacagcgc gaagtttgat aaaaccacgg
    25501 ttaccgcgct gaaggtgtct gctgaggaaa tcgccgccgc cagcgaacgc ctgagcgacg
    25561 agttaaaaca ggcgatggcg gtggcagtaa agaatattga aaccttccac actgcgcaaa
    25621 aactgccgcc ggtagatgta gaaacgcagc caggcgtacg ttgccagcag gtcacgcgcc
    25681 cggtggcttc agtgggtttg tatattcctg gcggctccgc cccgctcttc tcaacggtat
    25741 taatgctggc aactccggcg cgtattgcag gttgcaataa agtggtgctg tgctcaccgc
    25801 cgccgattgc cgatgagatc ctttacgcgg cgcagctgtg cggcgtgcag gacgtgttta
    25861 acgtcggcgg cgcacaggcc attgccgcgc tggcgtttgg tacggaatct gtgccgaaag
    25921 tggacaaaat cttcgggccg ggtaacgcct ttgtcaccga agcaaaacgc caggtaagcc
    25981 agcgtctgga cggtgcggcg atcgatatgc ccgcaggccc gtcggaagtg ctggtgattg
    26041 ctgacagcgg cgcaacgccg gatttcgtgg cttctgattt gctctcccag gctgaacacg
    26101 gccctgactc acaggtgatt ttactgacac ctgatgctga tatggcgcgt cgcgttgccg
    26161 aggccgtcga acgccagttg gcagaactgc cgcgtgccga aaccgcacgt caggcactga
    26221 gcaccagccg cctgattgtg actaaagatt tagcgcagtg cgtaacgatt tccaaccagt
    26281 acggcccgga gcacctgatc attcagaccc gcaacgcccg cgaactggtc gatggcatca
    26341 ccagcgccgg ttcggtattt ctcggtgact ggtcaccgga atcggcaggt gattacgcct
    26401 ccggcactaa ccacgttctg ccgacttacg gttacaccgc cacctgttcc agcctcgggc
    26461 tggcagattt ccagaagcgc atgaccgtgc aggaactctc gaaagaaggt ttctccgcgc
    26521 tggcttcgac cattgaaaca ctggctgccg ccgagcgcct gaccgcccac aaaaatgccg
    26581 ttactttgcg tgttaacgcc cttaaggagc aagcatgagc accgtgacta ttaccgattt
    26641 agcgcgtgaa aacgtccgca acctgacgcc gtatcagtca gcgcgtcgtc tgggcggtaa
    26701 cggcgatgtc tggctgaacg ccaacgaata tcccactgcc gtggagtttc agctcactca
    26761 gcaaacgctc aaccgctatc cggaatgcca accgaaagtg gtgattgaaa attacgcgca
    26821 atatgcaggc gtaaaaccgg aacaggtgct ggtcagccgt ggcgcggacg aaggcattga
    26881 actgctgatt cgcgcttttt gcgaaccggg taaagacgcc atcctctact gcccgccaac
    26941 gtacggcatg tacagcgtca gcgctgaaac cattggcgtc gagtgccgca cagtgccgac
    27001 gctggacaac tggcaactgg acttacaggg catttccgac aagctggatg gcgtaaaagt
    27061 ggtctatgtt tgcagcccca acaacccgac cgggcaactg atcaacccgc aggattttcg
    27121 caccctgctg gagttaaccc gcggtaaggc gattgtggtt gccgatgaag cctatatcga
    27181 gttttgcccg caggcatcgc tggctggctg gctggcggaa tatccgcacc tggcgatttt
    27241 gcgcacactg tcgaaagctt ttgctctggc ggggcttcgt tgcggattta cgctggcaaa
    27301 cgaagaagtc atcaacctgc tgatgaaagt gatcgccccc tacccgctct cgacgccggt
    27361 tgccgacatt gccgcacagg cgttaagccc gcagggaatc gtcgccatgc gcgaacgggt
    27421 ggcgcaaatt attacagaac gcgaatacct gattgccgca ctgaaagaga tcccctgcgt
    27481 ggagcaggtt ttcgactctg aaaccaacta cattctggcg cgctttaaag cctccagtgc
    27541 ggtgtttaaa tctttgtggg atcagggcat tatcttacgt gatcagaata aacaaccctc
    27601 tttaagtggc tgcctgcgaa ttaccgtcgg aacccgtgaa gaaagccagc gcgtcattga
    27661 cgccttacgt gcggagcaag tttgatgagt cagaagtatc tttttatcga tcgcgatgga
    27721 accctgatta gcgaaccgcc gagtgatttt caggtggacc gttttgacaa actcgccttt
    27781 gaacctggcg tgatcccgga gctgctgagg ctgcaaaaag cgggctacaa gctggtgatg
    27841 atcactaatc aggatggtct tggaacacaa agtttcccgc aggcggattt cgaaggcccg
    27901 cacaacctga tgatgcagat cttcacctcg caaggcgtgc agtttgatga agtgctgatt
    27961 tgtccgcacc tgcccgccga tgagtgcgac tgccgtaagc cgaaagtaaa actggtggag
    28021 cgttatctgg ctgaacaagc gatggatcgc gccaacagtt atgtgattgg cgatcgcgcg
    28081 accgacattc aactggcgga aaacatgggc attaatggtt tacgctacga ccgcgaagcc
    28141 ctgaactggc cgatgattgg tgagcaactc actaaacgag accgttacgc ccatgtagtg
    28201 cgcaacacca aagagacgca aattgacgtc caggtgtggc tggatcgcga gggtggcagc
    28261 aagattaaca ccggcgttgg cttctttgat cacatgctgg atcagatcgc cacccatggc
    28321 ggtttccgta tggaaatcaa cgtcaaaggc gacctctata tcgacgatca ccacaccgtc
    28381 gaagataccg gcctggcgct gggcgaagcg ttaaaaattg ctcttggcga caaacgcggt
    28441 atttgccgct ttggttttgt actgccgatg gacgaatgcc ttgcccgctg cgcgctggat
    28501 atctctggtc gcccgcacct ggaatataaa gctgaattta cctaccagcg cgtgggcgat
    28561 ctcagcaccg agatgatcga gcacttcttc cgttcgcttt catacaccat gggcgtgacg
    28621 ctacacctga aaaccaaagg taaaaacgat caccaccggg tagagagcct gttcaaagcc
    28681 tttggtcgta ccctgcgcca ggccatccgc gtcgaaggcg acaccctgcc ctcgtcgaaa
    28741 ggagtgctgt aatgaacgtg gtgatccttg atactggctg cgccaacctg aactctgtga
    28801 agtctgccat tgcgcgccac ggttatgaac ccaaagtcag ccgtgacccg gacgtcgtgt
    28861 tgctggccga taaactgttt ttacccggcg ttggcaccgc acaagcagcg atggaccagg
    28921 tgcgtgagcg cgagctattt gatctcatca aagcctgtac ccaaccggtg ctgggcatct
    28981 gcttagggat gcaactgctc gggcgacgta gcgaagagag caatggcgtc gacttgctgg
    29041 gcatcatcga cgaagacgtg ccgaaaatga ccgactttgg tctgccactg ccacatatgg
    29101 gctggaaccg cgtttatccg caggcaggca accgcctgtt tcaggggatt gaagacggcg
    29161 cgtactttta ctttgttcac agctacgcaa tgccggtcaa tccgtggacc atcgcccagt
    29221 gtaattacgg cgaaccgttc accgcggcgg tacaaaaaga taacttctac ggcgtgcagt
    29281 tccacccgga gcgttctggt gccgctggcg ctaagttgct gaaaaacttc ctggagatgt
    29341 gatgattatt ccggcattag atttaatcga cggcaccgtg gtgcgtctcc atcagggcga
    29401 ttacggcaaa caacgcgatt acggtaacga cccgctgcca cgcttacagg attacgctgc
    29461 gcaaggtgcc gaagtgttgc atctggtgga tctgaccggg gcaaaagatc cggctaagcg
    29521 tcaaatcccg ctgattaaaa ccctggtcgc gggcgttaac gtcccggtgc aggttggcgg
    29581 cggtgtgcgt agtgaagaag acgtagcgac gttactggaa gcgggcgttg cacgcgtggt
    29641 ggttggctcc accgcggtga aatcaccaga aatggtgaaa ggctggtttg aacgttttgg
    29701 tgctgatgcc ttagtactgg cgctggatgt ccgtattgac gagcaaggca acaagcaggt
    29761 ggcggtcagc ggctggcaag agaactcggg cgtttcactg gaacaactgg tggaaaccta
    29821 tcttcccgtc ggcctgaaac atgtgctatg taccgatatc tcgcgcgacg gcacgctggc
    29881 aggttctaac gtctctttat atgaagaagt gtgcgccaga tacccgcagg tggcatttca
    29941 gtcctccggc ggcattggcg acattaacga tgttgctgcc ctgcgaggta ctggcgtgcg
    30001 cggcgtaata gttggtcggg cattactgga aggtaaattc accgtgaagg aggccatcgc
    30061 atgctggcaa aacgcataat cccgtgtctc gacgttcgtg atggtcaggt ggtgaaaggc
    30121 gtacagtttc gcaaccatga aatcattggc gatatcgtgc cactggcaaa acgctacgcc
    30181 gaagaaggtg cagatgaact ggtgttctac gatatcaccg cttccagcga tggccgtgtg
    30241 gtagataaaa gctgggtatc tcgcgtggcg gaagtgatcg acattccgtt ttgtgtggcg
    30301 ggtgggatta agtctctgga agatgccgcg aaaattcttt cctttggcgc ggataaaatc
    30361 tccattaact cccctgcgct ggcggacccg acgttaatta ctcgcctggc ggatcgcttt
    30421 ggcgtgcagt gtattgtggt cggcattgat acctggtacg acgccgaaac cggtaaatat
    30481 catgtgaatc aatataccgg cgatgaaagc cgcacccgcg tcactcagtg ggaaacactc
    30541 gactgggtag aggaagtgca aaaacgcggt gccggagaaa tcgtcctcaa catgatgaat
    30601 caggacggcg tgcgtaacgg ttacgacctc gaacagctga aaaaagtgcg tgaagtttgc
    30661 cacgtcccgc tgattgcctc cggtggcgca ggcaccatgg aacactttct cgaagccttc
    30721 cgcgatgccg acgttgacgg tgcgctggca gcttccgtat tccacaaaca aataatcaat
    30781 attggtgaat taaaagcgta cctggcaaca cagggcgtgg agatcaggat atgttaacag
    30841 aacaacaacg tcgcgaactg gactgggaaa aaaccgacgg actgatgccg gtgattgtac
    30901 aacacgcggt atctggcgaa gtgttaatgc tgggctacat gaacccggaa gccttagaca
    30961 aaaccatcga aagcggcaaa gtcaccttct tctcgcgcac taaacaacgg ctgtggacca
    31021 aaggcgaaac gtctggcaat ttcctcaatg tggtgagtat tgccccggac tgcgacaacg
    31081 acacattact ggtgctggcg aatcccatcg ggccaacctg ccacaaaggc accagcagct
    31141 gcttcggcga caccgctcac cagtggctgt tcctgcatca actggaacaa ctgctcgccg
    31201 aacgtaaatc tgccgacccg gaaacctcct acaccgccaa actgtatgcc agcggcacca
    31261 aacgcattgc gcagaaagtg ggcgaagaag gcgtggaaac cgcactggcc gcgacggtca
    31321 atgaccgctt cgaattaacc aacgaagcct ctgatttgat gtatcacctg ctggtgctgt
    31381 tgcaggatca ggatctggat ttaacgacgg taattgagaa cctgcgtaaa cggcatcagt
    31441 gagttgcggg ctgagcaggt gtgatgttgt tgtcacaccc ggagccattg cccgatgcgc
    31501 aagcttatcg tgcctacaca ttatctcaac acacgtaggc cggataagat gcgccagcat
    31561 cgcatccggc aaaaaaaacg ggcatggtgt caccaccctg cccgtttctc ttaaatgcac
    31621 aataatatta tttcgcgttg taattacgca gcgcgttacg cccaagcaca atccccgcgc
    31681 caaccatgcc gcccagcagc acagccagaa tcaaggtaat tgctttcttc gggctatcgc
    31741 gacgaatagg taacgtcggt ttcattacat agcggtaagc atgaatatca agatcatcag
    31801 cctttaagtt ttcaatatcc agcaggtttt gacgagtctg atagtagttt ggtgagaaca
    31861 ccaacggacg agtcgcctcg tgcttaatca tcgactccag tgcttcgctc cccaaaagga
    31921 acaacgtatc ttgcgtcaca tcttcagtct gctgaacctg tggctttgtc acctgcgcct
    31981 gattcgcata ctgcaacgct tcctgaatct gacggatacg cagatctttc tgttcttgag
    32041 cgaccacttc ctgcgtcttc agagaatcct gcaagttttt cattcgcagc gcgatgttgt
    32101 ctttcaggtc ttgttccagt tcttcattta cctggtcgtc aacttgctgg atatacttcg
    32161 ccagctgttt ctgcgcacct tcaggtgttt taccaacata agacaccgct aaaggtaatg
    32221 actgattttt aacggtaggc tcaatggtca gcttttctgc ttcttcctgg ttatccagcg
    32281 tttccgctaa tgctgaaaat gcagtgctgt aacgtccgat aagtgaagcc tgaatgtcgg
    32341 agactttcgg tactgccgga ccgtaaatca cattcatcgc gttggtataa ccagcgattt
    32401 gccccacatc aggttgagta acgatagctg ttgaagtcca cttttctttg gcaacggcca
    32461 gataccctat agccagggca atagccacaa tgacagaaat gatgatagtc atcttgccac
    32521 gccatagttg gaccagtaaa tcaatcaaat caatctgttc cggatcatgg ttttgcccag
    32581 aaacattatt attttctact ctcatcatta ccctaacgga cgaaataatt ttaagtcgcc
    32641 atagtttagc gataatcgcc atgaatgcta taggataaat gataaaaaaa atgaattatt
    32701 acaaagggaa cataagacaa tcagggcgaa aatcgccctg ataacaagat gttagtcact
    32761 gccaaagaga tcgcgggtgt agaccttatc tgccacatcc ttaagctctt ctgccatacg
    32821 gttggaaata atgacgtcgg cttgttgttt gaaggtggcg agatcacgtt ccagacgaga
    32881 gttgaagaat gtatcttctt tcatcaccgg ctcgtatatg atcacttcaa cgcctttcgc
    32941 cttgatacgt ttcataatcc cctgaatgga agacgcacgg aagttatctg acccgctctt
    33001 cataatcaga cgataaatac ccaccacttg cggtttgcgt gacaaaatgg catcggcaat
    33061 aaagtcttta cgcgtgcggt tagcatcgac aattgccgaa atcaggttat tcggcacaga
    33121 ctggtagttc gccagtaact gcttggtatc tttcggtaga caataaccac catagccaaa
    33181 cgacggattg ttgtaatggt tgccaatacg cggatcgaga caaacacctt cgataatctg
    33241 acgagtattc agacctaaac tttctgcata gctatccagt tcattaaagt atgctacgcg
    33301 catcgccaga taggtattag cgaaaagttt aatcgcttct gcttcggttg aatcggtgaa
    33361 cagggtcggg atattttgct taatcgcgcc ttcctgtaat aacgccgcaa aacgttctgc
    33421 gcgttccgaa cgctcaccga tgacaatacg tgacggatga aggttatcgt aaagggcttt
    33481 accctcacgg agaaattccg gggagaatat aatattttca gtacgatatt ttttatgcat
    33541 cgctgcggta aaaccaacgg gaaccgttga tttgatgacc ataaccgcat aagggtttat
    33601 ctcaactacg t
//
LOCUS       DAZFJL010000036        33502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFJL010000036 DAZFJL010000000
VERSION     DAZFJL010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.036"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(154..432)
                     /locus_tag="ACHJ8C_001739"
     CDS             complement(154..432)
                     /locus_tag="ACHJ8C_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5551606.1"
                     /translation="MSTFNTRKDRDRNGKAKPHKHSGIPTLTGKWRHPSMDCWVNYST
                     PGDHEWKRLYTTLRRRADDRRQCHRVMQGDDPEGIVWFPDCYPQIYYY"
     gene            complement(791..1081)
                     /gene="nadS"
                     /locus_tag="ACHJ8C_001740"
     CDS             complement(791..1081)
                     /gene="nadS"
                     /locus_tag="ACHJ8C_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO5551607.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(1082..1393)
                     /locus_tag="ACHJ8C_001741"
     CDS             complement(1082..1393)
                     /locus_tag="ACHJ8C_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5551608.1"
                     /translation="MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            1622..2530
                     /locus_tag="ACHJ8C_001742"
     CDS             1622..2530
                     /locus_tag="ACHJ8C_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5551609.1"
                     /translation="MALEKGIAELVEEFIAAGRPSSRQQSITQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRIIAVQYQLAPEHTFPAAHDDAQRGAELVRQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPIWRNDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(2594..3535)
                     /gene="fabY"
                     /locus_tag="ACHJ8C_001743"
                     /gene_synonym="yiiD"
     CDS             complement(2594..3535)
                     /gene="fabY"
                     /locus_tag="ACHJ8C_001743"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO5551610.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3580..4017)
                     /gene="dtd"
                     /locus_tag="ACHJ8C_001744"
     CDS             complement(3580..4017)
                     /gene="dtd"
                     /locus_tag="ACHJ8C_001744"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO5551611.1"
                     /translation="MIALIQRVTRASVTVEGEVAGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(4014..4886)
                     /gene="yihY"
                     /locus_tag="ACHJ8C_001745"
     CDS             complement(4014..4886)
                     /gene="yihY"
                     /locus_tag="ACHJ8C_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO5551612.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4880..5479)
                     /gene="yihX"
                     /locus_tag="ACHJ8C_001746"
     CDS             complement(4880..5479)
                     /gene="yihX"
                     /locus_tag="ACHJ8C_001746"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO5551613.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPDD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5620..6507)
                     /locus_tag="ACHJ8C_001747"
     CDS             complement(5620..6507)
                     /locus_tag="ACHJ8C_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO5551614.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQVGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMTSSANSLVVFDKQPDATCINPLTQAPNAINLTPEF
                     VTPDKPLVIETRWQFTPES"
     gene            complement(6497..7846)
                     /locus_tag="ACHJ8C_001748"
     CDS             complement(6497..7846)
                     /locus_tag="ACHJ8C_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5551615.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIASFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(7945..9378)
                     /locus_tag="ACHJ8C_001749"
     CDS             complement(7945..9378)
                     /locus_tag="ACHJ8C_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5551616.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(9448..10458)
                     /locus_tag="ACHJ8C_001750"
     CDS             complement(9448..10458)
                     /locus_tag="ACHJ8C_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFO5551617.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQQLKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(10491..11378)
                     /locus_tag="ACHJ8C_001751"
     CDS             complement(10491..11378)
                     /locus_tag="ACHJ8C_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO5551618.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKARGMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(11403..12281)
                     /gene="yihU"
                     /locus_tag="ACHJ8C_001752"
     CDS             complement(11403..12281)
                     /gene="yihU"
                     /locus_tag="ACHJ8C_001752"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO5551619.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNTQAVDALVTQG
                     ATAAQTPAEAAASAEFIITMLPNGDIVRQVLLGEKGVCETVSSDALVIDMSTIHPLQT
                     DALIRELQEKGINMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARKTGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            12454..13350
                     /locus_tag="ACHJ8C_001753"
     CDS             12454..13350
                     /locus_tag="ACHJ8C_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO5551620.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            13390..14178
                     /locus_tag="ACHJ8C_001754"
     CDS             13390..14178
                     /locus_tag="ACHJ8C_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5551621.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFTA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(14238..15503)
                     /gene="yihN"
                     /locus_tag="ACHJ8C_001755"
     CDS             complement(14238..15503)
                     /gene="yihN"
                     /locus_tag="ACHJ8C_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5551622.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEASSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(15605..16585)
                     /gene="yihM"
                     /locus_tag="ACHJ8C_001756"
     CDS             complement(15605..16585)
                     /gene="yihM"
                     /locus_tag="ACHJ8C_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO5551623.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKKSIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNN"
     gene            complement(16593..17303)
                     /gene="yihL"
                     /locus_tag="ACHJ8C_001757"
     CDS             complement(16593..17303)
                     /gene="yihL"
                     /locus_tag="ACHJ8C_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5551624.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KSRTRDIPETLVPLITVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(17520..19343)
                     /gene="typA"
                     /locus_tag="ACHJ8C_001758"
     CDS             complement(17520..19343)
                     /gene="typA"
                     /locus_tag="ACHJ8C_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO5551625.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            19716..21125
                     /gene="glnA"
                     /locus_tag="ACHJ8C_001759"
     CDS             19716..21125
                     /gene="glnA"
                     /locus_tag="ACHJ8C_001759"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO5551626.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            21299..22348
                     /gene="glnL"
                     /locus_tag="ACHJ8C_001760"
     CDS             21299..22348
                     /gene="glnL"
                     /locus_tag="ACHJ8C_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO5551627.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRITESIHKVAERVVTLVSMELPNNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            22360..23769
                     /gene="glnG"
                     /locus_tag="ACHJ8C_001761"
     CDS             22360..23769
                     /gene="glnG"
                     /locus_tag="ACHJ8C_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO5551628.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     SPSHMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            23881..23991
                     /gene="yshB"
                     /locus_tag="ACHJ8C_001762"
     CDS             23881..23991
                     /gene="yshB"
                     /locus_tag="ACHJ8C_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO5551629.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(24224..25597)
                     /gene="hemN"
                     /locus_tag="ACHJ8C_001763"
     CDS             complement(24224..25597)
                     /gene="hemN"
                     /locus_tag="ACHJ8C_001763"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO5551630.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLLFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(25786..26295)
                     /gene="yihI"
                     /locus_tag="ACHJ8C_001764"
     CDS             complement(25786..26295)
                     /gene="yihI"
                     /locus_tag="ACHJ8C_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO5551631.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(26414..26580)
                     /locus_tag="ACHJ8C_001765"
                     /pseudo
     CDS             complement(26414..26580)
                     /locus_tag="ACHJ8C_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26877..27509
                     /gene="yihA"
                     /locus_tag="ACHJ8C_001766"
     CDS             26877..27509
                     /gene="yihA"
                     /locus_tag="ACHJ8C_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO5551632.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     LVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(27891..30677)
                     /gene="polA"
                     /locus_tag="ACHJ8C_001767"
     CDS             complement(27891..30677)
                     /gene="polA"
                     /locus_tag="ACHJ8C_001767"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO5551633.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQPPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAARPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKEWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            31041..31973
                     /gene="yihG"
                     /locus_tag="ACHJ8C_001768"
     CDS             31041..31973
                     /gene="yihG"
                     /locus_tag="ACHJ8C_001768"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO5551634.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQEK"
     gene            complement(32014..33444)
                     /gene="yihF"
                     /locus_tag="ACHJ8C_001769"
     CDS             complement(32014..33444)
                     /gene="yihF"
                     /locus_tag="ACHJ8C_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO5551635.1"
                     /translation="MIRKSATGAIVALAVIWGGGTWYTGTQIQPGVEKFIKGFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKIVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDDSSLSNLDIEGKVEDIVLQFSPMNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQVNIINQGEVVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDMK
                     LPLNVATETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMLQLITIDNNTASLQLRY
                     TPGKVFFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         8450 a         8583 c         7991 g         8478 t
ORIGIN      
        1 ttttatattc aagaatattt tttgattggg tcttcatttg cttcatcagg atggtgtacc
       61 cgaaaaagtc agcagcttta ccgcaccaag gtttaaggtg aaatctacca ttcataacga
      121 ccggtcagtg ccgcaccaac cggtctttaa ttgttagtag tagtaaattt gtggataaca
      181 gtccgggaac cagacgattc cttccggatc atccccctgc attacccgat gacattggcg
      241 tcgatcatct gcgcgacggc gaagcgtagt atagagccgc ttccattcat gatcgcccgg
      301 tgtactgtag tttacccaac aatccattga agggtgacgc catttacctg taagtgttgg
      361 gatccctgaa tgcttatgcg gcttcgcttt accattacga tccctatctt tacgcgtatt
      421 aaacgtggac atttttagtc tcctgttaag agttctaaaa atgtgcttcc ttaaaatagt
      481 tcatcatttt cccttaattc gcatagcgtg cttttgcacg aatttccgat acgcggggac
      541 cattatgata gcgtcgatgc ccacacatcc aacaagaaca aatacaggga gtacgaaagc
      601 aaatacctga tgcttttttg cttccatcgc ctgcagtgcg ataacgacag cggatttttt
      661 ttaatcgcac catatgatgg cgacgttcag cacgattacg ggtcatagca aaactcctga
      721 acaggcggca ctatgccgct ttcataggag ttctgaatgc tggtgctttc tgtatggcat
      781 aaccatgcct ctaacggtta agtgcttgca gcacatgttg cggatcattg gcaatcgctc
      841 gcagcaaagc ctttgcaggc ccggtcggtg aacgtcgccc ctgctcccag ttacgcaacg
      901 tgtctacgct tacggaaata agatcggcaa atttagattg tgacaaccct gaagcgcggc
      961 gtatttcttt gattttcata gcatcaactt caaaagtccg tgaaggctgt cgcgttcctt
     1021 caataatttc attcatctgg gccatgctgt gagtcaaccg ctcaaaaagt gccttatcca
     1081 tttaccacct cgtatttaac aaccggagca ttgctttttc ctggggagac agatcatctt
     1141 taataccctt acgataaata agcagtaatc tgatctcata atgtttaacc tgatgaaagt
     1201 aaataaccct gacaccagca cgtttcccct ttcctcctga gacccatcga acctttctta
     1261 acccaccagt ttcaggtatt acctcaccat agtcaggatt caaggccagg aaaaactgaa
     1321 aacgactgaa ttcatcgtcg ttcagcagtt tttgaacatc ttcagtaaaa atttccgttt
     1381 cgatgaagag cataattata cgccattggc ttagagtaac aatccttttt gtacgtcatt
     1441 gacgtaccat tgaaaccact agcttactga tagtgctcca gcaaaaccac gtctctgccg
     1501 ggtttgtctt tacatttgcg aggcgctttc cagatttttc acgcccgtga atttccctgt
     1561 atccatccag caaaaacggg tgtataaaac tgtacattca tacagcaaaa tgagttctac
     1621 tatggcactg gaaaaaggaa ttgcagagtt agtcgaagag tttatcgctg cgggtcgccc
     1681 ctcatcgcgc caacaaagta ttacgcagcg cagagagggc tatatcgcca gcgctgtcct
     1741 tgcgggtgag acagaaacgc gggtagatat tcagactatc gaacttgaag ggatgacctt
     1801 acgaatagtg tcaccgctga atgccccgac attactcccg acgataattt attactacgg
     1861 tggctgtttt gttagcggcg ggttcgcaac tcacgacaac cagctgcgcc agttagccta
     1921 ttacgggcaa tgtcggatta ttgccgtcca gtatcagctg gcaccggaac atactttccc
     1981 cgccgcccat gatgatgcac aacgaggtgc agaattagtc cggcaacacg ccgaacgact
     2041 cggcgttgat aaacaacgaa taactcttgc cggtgacagt gcgggagggc atctggcgct
     2101 ggtaactgct ttgcgattga agcgtgctgg cgagtggcaa ccagcacaac tcatcttaat
     2161 ttatcctatg cttgatgcga ctgcgcattt cgaaagctac atacgcaatg gtcacgatta
     2221 catcatcaca cgcgacactt tactgagcgg ctttgagatg tacctgccag gcattgaacg
     2281 caggcatcct gaagccagcc ctatttggcg caatgatttc aatggcttgc cacctgtaca
     2341 tatcattacc gctgaatacg acccactttg cgatgaaggt gaagcgttgt atcaccgcat
     2401 gacagggcaa ggagtgcaat gcacctgcca acgctacctt ggcgttatcc acggattttt
     2461 tcagttaggt ggtatcagtg aggctgcgcg atcggcgcta cgggatgttg cctggagagc
     2521 gggaaggtaa ctccggacca gggcccagaa aaggttttca gattgaaaac cttttcagtc
     2581 aagtaaaacg cccctactct tcttcgtttc cgccctcttc atacggacca aatggcttcg
     2641 cgggcagaac gatatacgtg ccttcaaaca ctgcacccgg cgtctcgtcg ccaaagattt
     2701 ctacttgcat ctgcacccgt gcttttcgtc cgcgcgccag acggtcgaga tcgccactta
     2761 aggcaccaag gtcggctacc gcatggggtt ttccgctaat cggtttgcta tagcggatat
     2821 gcgcatccgc cagaataatc gttccgccga ggtggcgttc gcgcagcatc agccagataa
     2881 gcccccaacc agtgagcgtt gccagcgaga ataaactccc ggcaaacagc gtgtgatgcg
     2941 gattctgatt gcctgtttcc ggcatggtgg tgataaattt ttgcccggta tattgctgaa
     3001 tgcgcacacc cattttttca ctcagcggga tgtgttcata ccacgcttgc tggagctggg
     3061 cgcaccagtc gccgcgatga agaatatcat ccagagtggc aacaggtttt atcatcaaaa
     3121 aatggcgaat cggcgtggtg gttggcgtgg tgatttcacc ctgattaaca aaccccagct
     3181 tggcgaaaaa ctccaccgcg tcttcacggg cgctacaggt cacgcgctta acgccttcct
     3241 gacgtgccac cgactccagg gtcatcgcca tcagcgtgcc taaccctttg tcctgcacgt
     3301 cgggatgaac ggccataaag cgaatggacg cttcattgtc ggcattaata tacagtcggc
     3361 ctaccgctac cagattaccc tgctcgtcga cgaccatctg gtgatgcgcc atcgcatccc
     3421 acgcgtcgcg ttccgaacct tttggttgat gcaggggctt acgcaacatt tcccagcgaa
     3481 actgatagta acgctctaat tcttcttctg tttgtggaac ccgaaggtga tacatagcga
     3541 tactctctct tgtttcccgt gacaaccctg gaagctggct catacctgca accagaatgt
     3601 cacggggcca tcattgacca gcgatacctg catatccgca gcgaagcgtc ctgtttgcgt
     3661 gttcatctct tgctgacggc agcgttcgac gaaatagtca tataacgcct ctgcgcgatc
     3721 cggtgatgcg cctttggaga aacttgggcg catcccccgt tcggtatctg cggcaagggt
     3781 aaactgggaa accaccagta cactgccgcc cgcctgttgc acgttgagat tcatcttgcc
     3841 ttcggcatcg ctaaagatgc ggtagccgag cacacgctcg cacagacggt ttgctttctg
     3901 ttcgtcgtca tccttttcga cacccaataa caccaaaagt cccgcgccaa tttcacccgc
     3961 cacttctccc tccacggtga cgctggcacg ggttacgcgt tgaattaatg caatcatggt
     4021 tcgtcgtctt cttcttgttc agcagcttgt tttaatttgc ggtattcccc gagagtgaca
     4081 gtaatttccg cgccaagcaa gacgatacac catgtccagt agacccaaac aaagagaatg
     4141 gggatcaccg ccagcacacc gtaaatgagc tgatatgatg ggaacatggt gatataaagc
     4201 gcgaaacctt tctttcctgc ttcgaacagg agcgcggcga caaacgcgcc gacaatcgcg
     4261 tcgcggttag gtacacggat ggtaggaacg atgctgtaca gcagccagaa agagatccac
     4321 gacaacagca gcggaaaaat acgcaacaca ttatcgatga cagtattgag atcgctcgcc
     4381 cagcgcagag agagcagata ggagctgatc gccagactgg cccctgccaa cagcggcccc
     4441 agcgttaaaa tcatccagta cacggcaaac gagtaaattt tgggtcgcgc tcgtttactg
     4501 cgccagatgg tattcaacgc gctatcgatg gagtacatca acaataacgc cgtgacgatc
     4561 agcccgcacg ccccgacggc ggtcattttg ttggaattgg cgacaaactg ttcgatatat
     4621 cgctggataa catcgccagt agcaggcaga aagttggcaa aaataaagtg acgcaactga
     4681 atgctgacgt cggaaaacat gggaaaagcg gcgaaaagcg caaaaacaac ggcaaccagc
     4741 ggcactaatg agagcaacga cacataggca aggttacctg ccagggttgt catgttgtcc
     4801 tcatcaatgc gttgccagag tagttttagc caggcccata gtggacgggt acgatgcctg
     4861 gctttgtcct gaatggtttt tagcataaca ccttcgcgaa atagtccggg atggtggttt
     4921 tatctttcac cagaatacta gtaatgccta gctgattagc tccttctata ttatcggcgt
     4981 tatcgtcgaa aaagaccgta tcgtctggtg aaaaaccttc tgcctgcaaa acatgctgat
     5041 aaattcgtgc ttcaggtttg cgcataccca agtcttgcga cagatagata tggtcagcag
     5101 catcacggat ttccgggtat tcctccggcc agaaggtagt atgcaggcgg ttagtattgg
     5161 aaagcaccac cacgcgatgc ccctgctcac gcagtttatg catgatggcg atcacttccg
     5221 gtcgtagcgc aacaaatacc gcctgccagc cgtgagagaa ctgctcgtag cttagcggta
     5281 gagccatctc atgacacagc gcctctgcga acgcttcgtc gctaatttcc ccacgctcat
     5341 gctgatgaaa cgcctcgccc atgtggaaac tcttcttaag cgtagccagt ggaacacgcg
     5401 ttaaatcgct ccaggctccc agcacacggt taaagtcgat atcgacaatc acattaccta
     5461 aatcaaagat atagagcatt tttgcctcct ttcgcgccac aagaaattaa ctgtagcggg
     5521 aaaggatggg tttggctatg tggcaataag ggtaatcttg tgatgctgat tgcgaaaaag
     5581 cagagtaatt tcactggctt tttcgcaata gatgaatggt taagattcag gtgtaaattg
     5641 ccaacgagtt tcgatgacca gcggcttatc tggtgtcaca aattccggcg ttagatttat
     5701 tgcgttaggt gcctgagtca gcgggttaat acaggtggca tcgggctgtt tatcaaatac
     5761 caccagactg tttgcagaag aggtcatggt catcgcgagt ttaccaggcc acagtaactt
     5821 aaccttcacc ccgtcataaa aaccaaaaca gtcatcccag ggaccgactt gaggagagat
     5881 acgatttcca gttggcagtt catcactacc cgcttcttct tgccagtccg catcaaaaag
     5941 cacctgcaac gattctgtat tttgcggagt cagctttttc gcaaaccacg gatgccagcc
     6001 tgcggaggcc gggaatgtat cggcatatga atggacttcc agttgcaaaa ccagcgcatc
     6061 gttctccagc aaaaaagtct gaatcacttc cccttgccag ggccacggtg aagccagggg
     6121 catacgtaaa gtcagggaat ctgctgtttt gtcgatgatt tcccacgttg agtaacaagc
     6181 cataccatgc aacgcatgtg gtggtttatt ggcgggtaaa gaataacatt gcccaccagc
     6241 attcagcgtc gcattcccca gtcgccctgc ccacggcacc attgggaaac agccatactg
     6301 aaaagctctg cgctgcggct gccattggcg taatacttca gaaccaaatg cctttaacga
     6361 ggttatcctg gcaccatcat gcggataaat ctgcaggtga atattgcctt tttccagcgt
     6421 cactaactct cctacaggcc caggccatgg cgcgtcaaac gcctgttgta gcgccaggta
     6481 atgctcgttt tttcgttcag ttttcatgag attgttccct gatgtagcgg taagaagcgg
     6541 tttcttgttc atcggttggc tcaatcagcc agacgcaaac aaaggaaaga actgagacag
     6601 caaataaaaa cagagcgaca taccacggtt ttcctccccc tagcgccagc aaacttgagg
     6661 cgataagggg gcttagtccg ccaagaatcg cggaaaattg ataagcaaaa gaaaggccgg
     6721 tatatcgtac tttggtaccg aacatgcggg tgaaaagtgt tggctgcacg gcaaacatca
     6781 tcgtcggacc taagttgtag ccaaggatca tactgcacca gatgatgagc gtacttttgc
     6841 tatccagtaa taagaagaaa ggaaacgcga acaggacaca aaatcctgct cccgacaaat
     6901 acagactttt ttgacctata cgatctgata accatccaac caatggacaa ctgaaaatac
     6961 cgatcgccga ggcaatcaac atacccgtca ggggaatatc tcgcgacaat gctaattggc
     7021 tggaaatata gacaatacca aaggcgttaa taatattaga ggagacactt tccgccagcc
     7081 tcgcacccag tgccaataaa atatttcgtg gatgtttttt gaataattca atcaacggag
     7141 gaacggactt ttctttatta ttcgtcgttt tttgcttttg aaaatccagc gtttcttcgg
     7201 tatgcaggcg gataaacagc ccaacgatca acatcaccgc agaaagccaa aacggaatgc
     7261 gccatcccca ggagagaaac tgctccgaag taagaaaatg gttacagaga gcaaaaatac
     7321 cggttgccag cataataccc acagacgcag ccgtttgcgg gagtgaacct aaaaatccgc
     7381 gtcgggattc gggcgctgat tctatggtca tcaatgccgc tccgccgtac tcgccaccaa
     7441 gaccaaagcc ctgaatcaaa cgcagcacca ttaatacaag cggtgcccaa atgccaattt
     7501 cctgatacgt cggaataaaa ccaatcaaaa atgtggagca accgacaata gccagcgtcc
     7561 agataagtgt tatttttcgc ccgattttat cgccaaaatg accaaagacg atgccaccta
     7621 aaggacgagt cagaaaacca acaccaaagg tcgcaaagga agctataaga cctataaaag
     7681 gatcgctgtc tggataaaac agcggtgcaa ataccagtcc tgctgccgtg ccaaaaataa
     7741 agaagtcata ccattccatt aacgcgcctg cgaaactgcc cataacgaca cgtctcatgg
     7801 ctttaccgga aagcccttca cgcggtgcgg gtaacttttc agacataatt ttcctctcct
     7861 ggtaggcgag aaatctggga tacgaaccac taaacgtggg cagtttgaat taatgcgcat
     7921 cggcttaaac cgatgcgcat taaattattt cttcaattca gccagaacct ctgcgtcctc
     7981 agcatcaaca tttgcaccca gtggttccca attaatcaat acacaggtca ggaatcccag
     8041 gaatggcacg attgccaacc agtaaaacgc ttcagttttc aacgcacccc acagcatcgg
     8101 ccagaaaatg agtcctgcaa ttgcccctgt gcgcataata gcgcgggcaa atccgacccc
     8161 agtagggcga atagcgggag gataagagag tgtggcaatg gtcatgccca gcccaccagg
     8221 acccgctgaa tggaagaaaa taattgatcc caataaccct aaagacagcc agggattagt
     8281 cgttgccagt gcgcctaacg acagcaatgc taaggtcaca acagcaaatc catacatcga
     8341 ctgccggcga gtacccagtc gttggagaat aaacgagccg atcatcccgc ctgtcacccc
     8401 acaaagggca ttcacgacgg cagaaccggt taacgcgcca gtaagcccgc cacttattat
     8461 tcccgccaac gtaagaggaa gataaacacc tacggcgtta tattgccacg cctgcatggt
     8521 tgccacgaca cagccaagaa tggtgcgttt gcggtaacga tcattaaata agatgccata
     8581 tccaccactg tatttattct ctgctttttc cgagaatttc gcttctgttg tacccgatgc
     8641 cgcaacgcga gcctgaacac cataacgttt tcccagaatg tcgcacgctt cctggtaacg
     8701 cccgacgcgt gcggcccaca ttgcggattc accaataaag aagtaacgca aaatcatgaa
     8761 gatgactgca aaaatagctc cgatgaatat tccgtaacgc cacaattgtt cttcagcgat
     8821 acccgacaga agcagcggca aaagcaccaa ataaacaacg gttgtcgaaa cataccaggc
     8881 catttgccag aggttgaccg acgtgccggt tttcttcgac gttgaaccac gaagctctgc
     8941 cacggcattg gttgccaaag ggaagtcgat gcctaaacca aagcccatga tgacgcggca
     9001 caccagcacc caccagatac tgggagcaat agctaccgct gcagcaccga tggtgcacag
     9061 ccccatgcca ataataaatg cccgcttttg cccaaagcgg tttattactc ggttcgctaa
     9121 taaaccgcca attaacgcgg cgattaatac tgatgcattg acggttgcag caagacccgg
     9181 cgaaatgccg aattgagcgg caatgtattt attaccaaac cccaccatcg ccgcctggta
     9241 ggcatcaatc aatatgccgc ccaaagcaat aaaaataacg ccgatactgg tgttgattgc
     9301 agagttctta ttaacaatat caattacgtc ctgtggccga ctaattgaat aagttgcagg
     9361 actggtgatt tgactcatcg cagtgtcctt tttaattgtt gtagggtggc gctgacgtga
     9421 cacagcgcca gacagagatt tagaaaatca ttccggcgca aattgcagca tgacctttat
     9481 ggagtcattt tgctgcgcgc gttcgaatgc cgccgctgca tcctcaatgg caaataaatc
     9541 ggtaaccagt tcactggtat caaaccgccc atccgccagc cattcgataa cagcatcgaa
     9601 atcttcaggg atgtacatgc cagagttgag caagtcacgc tcgaaacgtt gcatcatcgg
     9661 taatggtatt tcacgcggcc cacttggtac gcccatgcac acgacggtgc ctccggcata
     9721 tacacgggta catgcatcgt taagcgtggc ctgcgctgca acgacatcaa tcgcccccgt
     9781 aaatcgcaca tctgcggcca gctcacctgg tgcccaaact tctgctgcgc ccagttttaa
     9841 cgccagcgcg cgtttggcag cgtcaggttc aatcacggta atcttacctg cccccataat
     9901 gcgcagtgcc tgcacgatgg ataagcctat cgttccggca ccaatcacca gtacgctttc
     9961 cagagaagct ttcggcattc ggttaacgca gtgacgagca caagccgccg gttcggcaaa
    10021 ggccaacact tttagcggta aagaggctgg tgcgacatgg caattacgcg caggaacaat
    10081 gtgttgtgag cgagcaaagc ccggtgcgcg aaaaccagca acctgcggtg gttcacaaag
    10141 attaaaacgt cctgctttgc aggctcggca ttccatgcaa gccatgatgg gatcgaccac
    10201 aacatgatcg cccggctgta catttttaac gtcgctgccc acttccgtaa cgcgcgctgc
    10261 aatttcatga ccggggacga caggcggttt ggcaaacgga tgaccgcctt tcagaacatg
    10321 cagatccgaa ccgcatatcc cgtagaacac gggcgcaatc cgtacttcgt gcggtttaag
    10381 ttgctggtgt ggtacttcaa catattgagt ggtgactttc ccaatatctg aaaataaaac
    10441 ctgggtaatt ttatccattg ttattctcca gaatcgatat ccatagggga ttagcgttgc
    10501 gcgatacctt catcgacaat ttcagccagc cgttgcaagc gtggaacgga gacatcgcgc
    10561 agcatggttt gtggatcttg tgcaccaaca accgacgccc aaaccgctcg tccagccagg
    10621 aagcccgacg caccaccttt catggcaata cttactgcac ggccaaaaat atcggcatca
    10681 acgccagaag aaagaattac ccacggcatc ttcatctgat cgttaagttg ctgacaggca
    10741 gcaagtaagg ttttttcatc gcctttgccc cctaatggca tctcggcttt gtaaagatca
    10801 gcctctgttc caccaagctc tgcggcggcg gcaacaatgg cgctttcacg atcaaagtcc
    10861 caaccgcgac gaggtggacg caccacaggt tcaataatgc taaccagccc agcactacgg
    10921 cagcgacgaa caaatttatc aaccattgcc agacgttcct ctgcgggttc atcttcacgc
    10981 cagagtacaa gtaatttcat tgccttagcc cccattccac gggctttatg cgggtcgacg
    11041 gacatatcta tttcaacgct atcaaccggg atgccattac ctgggataaa aagatcggca
    11101 gcgacaatca ggccgcaaga attagccaca gcgccctgct cgactatttg ttcaagacaa
    11161 aattgtttat ccgccagaac ggcagaagca taaggagaaa ggattcgggt cgcggcaact
    11221 ttaaaatcgg tgagtacact gtcggcgatg ggttttggct gccctgctgc cgcaaacatc
    11281 aggcgcatgg cctcacgctg atcgacagct aacatggcga atccaccacc tgggcgggag
    11341 atatctttca gcgtaaatgg ggtgaagtgg gatgacatag aaactcctgt taatagataa
    11401 aattaaagac cagcctgctg gcgaacctga tttagcaggg cgctccagtc ctgccgccct
    11461 ttgccgtctt gacttgctgc ggcgtaataa ctttctgctg caatccccat attgagcggc
    11521 gcccccgttt tccgagcgac gtcaactgca atacgcagat ctttcaatgc caggtccacc
    11581 atgaaggcgg gagaaagatc gcctttcagc actttgccag gccaggtggt cgtaaaatgt
    11641 cctttccctg ctgccgtacc actcatcacc ttaattgcga catcaagatt taatcccagt
    11701 gactcacata acacagcggc ttcggaggac agcgcattaa gcgcaatgct catatagttg
    11761 ttaattagct tcacgcggat ccccattcct gggccaccag cctcgaccag ctcattaccc
    11821 atgcacatga gaatcgggcg tgcacgcgca atctgctcat cagtgccgcc cgctaaaatc
    11881 agcaatgtgc catcaatcgc attcactgac gttctgccca cgggggcgtc catcatattg
    11941 atgccttttt cttgtaattc ccgtattaat gcatcggtct gtaagggatg aatagtcgac
    12001 atatcaatca ctaacgcgtc agatgaaaca gtttcacaga cgcctttttc ccccaacagc
    12061 acctgtcgaa caatgtcccc attgggaagc atagtgatga taaattcagc gcttgctgcc
    12121 gcttccgctg gggtttgtgc cgcagtggcc ccctgcgtaa ctagcgcatc gacggcctgt
    12181 gtattgacat caaatacctg taatgaatgc cctttttgca atagattgtt agccattggg
    12241 ctacccatct gccccagccc gagaaatgca atggttgcca tgaatactct cctgtctgac
    12301 attgacaaaa taatgacaat tgaaaattcg ttttcgtctg tttttgagca tatttgtaat
    12361 ttatgttcaa aaacacgaca cgcatcacta atttgatcat ttgtgtcatt taaattgacc
    12421 aaagacgaca aaattaaaca ggacaagaca aatatgacca gaattgcatg cgtgggtatc
    12481 acagttcagg acagaattta ttctttgccc actctgccgg agggtggcgg gaaatatcag
    12541 gcaaatcatt atcttgagat tggtggcggc ccggcagcaa cagccgctgt tgcaatcgca
    12601 aagcttggcg tggaggttga tttcatcggt cgcgtcggcg atgacagttg tggaaatacc
    12661 ttacttgcag aacttgaggg ttggggcgtc aataccgcat tttgccgccg atatcctaac
    12721 gcccgttcgt cgcaatcagc gatcctcgtt gatcagcatg gcgaacggat tatcgttaat
    12781 tatccaagtc ctgatctcgg tactgatgcc gaatggttag aagcgatcga tttctctcgt
    12841 tatgatctga ttctggccga tgttcgctgg catagcggta ctgaaaaagc attctcgcta
    12901 gcgcgccttg cgggtgtgac aacgctttta gacgccgata tgacaccgca ggatatttct
    12961 ccacttgtcg cgcttgccga tcacgccgtg ttctcaacac cagggctgaa acgcatgact
    13021 ggcctgcagt cacctgaaga aggtttgttc caggcaacga cacaaacagc aggtaaagtg
    13081 tatgttacgc tgggttctga aggatcgttg tggattgaag atggtcattt gtgtcaacag
    13141 gaggcatttt ctgtcaatgt ggttgatacc actggagcag gcgatgtttt tcacggcgca
    13201 ctcgcggtag cgttggcaga aaaaatgcca acaaaagagg ctattcgttt tgccagtgcg
    13261 gttgccgcga tgaaatgtac ccagcctggc ggtcgggccg gcattcctaa tcgtgagcaa
    13321 accgaatcat ttttgtcact ttatgcgtaa aatggtaaac atctaccgag gaggggcaag
    13381 ggagtattca tgagtatcat cgaagtgaca ggtaatccac gccatgacca actcgttcat
    13441 ctaatcgcag aacgcggtta tatgaatatt gaagaactgg cacaactgct ggatgtttca
    13501 acgcaaaccg tgcgccggga tatccgaaaa ctcagtgagc agggcttgat cacccgtcat
    13561 catggagggg ccgggcgcgt ctccagcgtc atgaataccg cttttgagca acgggaactt
    13621 tcgctcaccg ccgagaaacg ggcgatcgct gaggcagtcg ccgattacct tcccgaacgc
    13681 tgtaccgtct ttatcaccat cggtacaacc gtagaagccg ttgccagggc attactcaac
    13741 cggcgtgatt tacgcattat cactaatagc ctgcgtgtgg cacagattct ttataagaat
    13801 caggatattg aagtgatggt gccgggaggc actttacgcg ctcataacgg tgggattatc
    13861 ggcccaggag ccgtggattt tattgaaggc ttccgcgcag attatttaat caccagtata
    13921 ggtgccattg aacatgacgg caccctactg gaatttgatg tcaatgaagc gttagtcgcg
    13981 agaacgatga ttaaacatgc gcggaataca ttgttagtgg ccgatcatac gaagtttacc
    14041 gcgtctgctg ccgtttcaat tggcaatgca cggaatgtca gggctttttt tactgatgcc
    14101 ccgcctccca attctttctg ccagttgtta agtgaagaga atgttgaact ggtggttgcc
    14161 gagcaagaag tatcctgaaa ttgaaacttc ggttgatacg gttttcgttg gccggattaa
    14221 gcacatccgg ccactaatta agcttcagcc gccaacatct ctttcttacg ttggctaata
    14281 cgcttaacca gcattacgga aaccaccgca ccacagaatg cgaagcaggc cataatgcca
    14341 aacacgattt tgtagccaat aatccccgga tttaaatcca gaatgtagcc atacagactg
    14401 aagcagaaca tcgccggggc gtaaccaata aagctaccca acgccatcgc cgcgcctgtt
    14461 ttattttcag caatttttgc ttcgccgata ggtgcaaaaa atacggctcg ctgtgtgaag
    14521 actatagcgc caaagcccag cgtacatgcc atccctaaat agaccggcat actttcgtgc
    14581 ggcagcataa tcaacagtac gagcgcagcg gtactgatga taaaggtgta gcatagatat
    14641 ttactcggcg atttcaggat cttatctgaa atcatgccac caatcggtcc tccaatcatt
    14701 ttcagacagt attggttaat gatgccgtaa gcccccacca gagcaacggg caatagatag
    14761 atgtttttca ggaatggaat gaagaatgtt aggccacagt aaaccgcgta aacgaagaag
    14821 acgttgaaag cgataagcca gatggttttg tctctcagca ccgaggtcat actggtgttt
    14881 tgcgatgctc cgtcttcttt tttcacctca acggacgatg cctcttcttt gtcattcagg
    14941 acaaagaaaa taataatgcc tacggcaatc acaataaggg aatagaacca gatgcctgct
    15001 ttaaaaccca ataagccact gccaaaccag gtaaatactg ccaacgcaga aaatgccacc
    15061 acggtatcga caataccacg ccctgtttcg aagaagccaa acaaccgacc ttgttgttcg
    15121 ctattgccca atcgacttac cgacttgagc aagaccggcc agttcatcat gtcgcaagta
    15181 acaccaaata gcgcccagac aaagaggatg ccccaatacc ccggcattgt cgtcaaataa
    15241 acacccagta atcctgtcgc aatgagtgag aatgacatgg tgtatctgcg cggtagttta
    15301 tcggcaaaat aaatagacag aaaaaagccc actgtggtga caaatgagtt taccgacata
    15361 gcattaccaa tttgaccatt ggtcaaatgg aaatattcct gcatcgggat ataaaacgca
    15421 tctttcagcg acggtaattt ataaattgta ccgccacaca gtgttaatag gctaaataac
    15481 gcccatttct ttttcgtgag cataacattc cctaagcgat ttttaaaatc actgttttaa
    15541 aggcaataag ataccgacca ttcatatgga atggttttat taactgcgta tgagttaaaa
    15601 cttattagtt gttcagaaga tggtttgcct cttgtttgat ttgttgtagt acgttacgca
    15661 catgatttat ctgattaatt gcatcttctt tttctttacg taaccgcgca tccagtaaat
    15721 gattttctgg caatggtgtt tcactcatct ggcgataagg gatccacgga ttatcatctt
    15781 cgtcttcaaa gcggaacgcc ggtgcataat aatcaacctc ttcttccagg ccatatgccg
    15841 tcacctgcgg ctcatcatca cccaggcaga taaggtgcgt taataacgct ttgaagggca
    15901 tatccccctg acctgaaata caggctttat gtcccaggcc accctgttct ttaacgatca
    15961 aagcatcttt gatatggacc tgagtaatat gcggtgccat cgtttttaaa gcgtcgatgg
    16021 gatgctcatt tgcattgatc atgttcgcaa aatcaaacag taaggaaaga gactccatct
    16081 cactttcttt gaccagcgac accagctcat gactttttaa atcttcatgc tgctcgatcg
    16141 taaaagtcag gccgctgtcc tgatacgttt ctcgtacata ggcaatgtcg ttagcgataa
    16201 tcgataatac gtcgcgcaaa ttaccttcat aacgcggata aaaacgtacg gaggatgccc
    16261 cagttttcaa cgcgatggcg acggcttcgt caatagattt tttatctgag gcgctggttt
    16321 caatatgaat atcaaggttc agacggcggg ctttatcccc aaaggcagag agttctttat
    16381 cgtccatatt accaagagaa aaacgctctc catccagaac atggattttg acgccacgta
    16441 gattattttc acttgcgata tcaagtaaat cagcaggcaa cactctttcc agtcgcatat
    16501 ttaaatgaaa ggcataagca tgtaaataaa gaggaagagt gtcgacacgt tgtagaatct
    16561 ttcttgcgtt atttattgtc accataaaac acctacagat taatgatgag gtctacggcc
    16621 tcaaatcgcc aatattcaat atctatctgg acaatattgc cctgctggtc ggcgcgatgc
    16681 ttttcaatta agatcgctgg catacccgca gaaccgccaa gatacttgct gatatcgccc
    16741 ggcattctga cgggtttgaa aatcaatttt tttactaccg tctctttttg gtagcacttt
    16801 tcccagactg cagaaaatga gtggttttca agttgttcaa taaaacccgg ggcaacttca
    16861 ggattaatat atgtttcgtg atagaaaact ttatgtcctt ccagcgctcc ccagccggtg
    16921 atgcgataga gttcagttga tggtgtcact gtaatcagtg gcacgagcgt ctcgggaata
    16981 tctctggtac ggcttttctc ggtaaacccc caggaaggct cccgcccttg ctcaattgca
    17041 gcccgctgaa agctcgccga cagctctgga ctgtaattaa atcgcggctg ggtcacgaac
    17101 caccctttac gatccttacg gaagattttg gattctgcct cgaggttcaa caacgcctgg
    17161 cgcagcgtca tacgtttaat gcccagcagt tctcccagct ctctttctga agggagtttt
    17221 ccacccggcg tggtgatgcc atctttcaac caccgatcca gtttctcttt tgcattttct
    17281 acggtggatt gattctccgc cattgtttaa tccttttggt ttaaaccaga taaggcatct
    17341 tacgcttacg ccaaaaaaga aagcaattaa cggcgtatga ctttgcagat aattgtgaca
    17401 aagctctcta ttgacgtaat cacagactta taagggagat actcgcattg gtgtgcgctc
    17461 acgacaggca aaaaaaaacc tgccatcgct ggcaggtttt ttatgactaa aaaacgaaat
    17521 taatcgtctt tcggtgcgcg gttagcgcga cgacgatcgt tttccgtcag atgacgttta
    17581 cgaatacgaa tagaggtcgg agtcacttct accagttcgt catcatcgat gaactccaga
    17641 gcttgttcca gagtcatgcg gataggcgga accagaacaa cggcttcgtc agtaccggaa
    17701 gcacgcatgt tggtcagttt cttaccggtc aggcagttta cagtcaggtc gttagagcgg
    17761 ctgtgaatac cgataatctg accttcgtaa acttctgcac cgtgaccgag gaacagctta
    17821 ccacggtcct gcagaccgaa cagcgcgaac gcgaccgctt taccctgacc gttagagatc
    17881 agtacgccgt tctgacgctg gcccacttca cccggacgta cgtcgtcgta gtggctgaag
    17941 gtggagtaca gcagacccgt accggaagtc atggtcatga actcagaacg gaagccaatc
    18001 agaccacggc ttgggatcac gtagtcgaga cgtacgcggc ctttaccgtc tggattcatg
    18061 tttttcaggt cgcctttacg ttcgcccagc gcctgcatta cagaaccctg atgctgttct
    18121 tcaacgtcca gagtcacgtt ttcatacggc tcttgtttac gaccgtcgat ttcacggaag
    18181 ataactttcg gacgggatac cgccagttcg aaaccttcac gacgcatgtt ttcgatcaga
    18241 acagacaggt gcagttcgcc acgaccagaa acgcggaacg catcggcgtc ttcggtttct
    18301 tctacgcgca gcgcaacgtt gtgtaccagt tctttgttca gacgatccag gatctgacga
    18361 gatgttacga acttaccttc tttaccgcag aacggcgagg tgttaacgca gaagaacata
    18421 gaaacggtcg gctcatcaac ggagagtgcc ggcagcgctt caacgttttg cgtgtcgcaa
    18481 acggtgtcag aaatgttcag ttcgcccagg cccgtgatcg ccacgatatc gccagcttcc
    18541 gccagatcgg tttcgatacg ttccagaccg aggtggccca gcactttacc gactttcgcg
    18601 ttgcgggttt tgccttcgct atcgatgata gtaacctgct ggttcggctt cactttaccg
    18661 cgcttgatgc ggccaatgcc gataacgcca acatagctgt tgtaatcgag ctgagaaatc
    18721 tgcatctgga acggaccgtc aaggtcaacg tccggcgcag gaacgtggtc aacaatcgcc
    18781 tggtacagcg gggtcatgtc ttccgccata tcttcgtggt ccagacccgc gataccgttc
    18841 agcgcagaag cgtaaacgat cgggaagtcc agctgctcgt cggtcgcgtc gaggttaacg
    18901 aacagatcga atacctgatc cacaacccaa tcaggacgcg cgccagggcg gtcaactttg
    18961 ttgataacaa caatcggctt caggccgtaa gcaaacgctt ttttggttac gaagcgcgtt
    19021 tgcggcatcg ggccgtcaaa tgcgtcaacc accagcagca ctgagtctac catggacatt
    19081 acacgttcaa cttcaccacc gaagtcggcg tgccccgggg tatcaacgat gttgatacgg
    19141 taatcattcc atttgatagc ggtgtttttc gcgaggatgg taatcccacg ctctttctcc
    19201 aaatcgttgg agtccatcac gcgctcttgg gtttcggcac gagagtcgaa cgtaccggat
    19261 tgttggagca gcttgtctac cagggtggtt ttaccatggt ctacgtgcgc gatgatggcg
    19321 atattacgca atttttcgat cacaactttg cctcaggcat tagaaatagc gcgttattgt
    19381 acacggatta atcgcactac aaaacaggat cacaaacatc ctccgcaaac aagtattgca
    19441 gagtcccttt gtgatcgctt tcacggagca taaaaagggt tatccaaagg tcattgcacc
    19501 aatatggtgc ttaatgttcc cattgaagca ctatattggt gcaacattca catcatggtg
    19561 cagccctttt gcacgatgtt aagcatgata acgcctttta ggggcaattt aaaagttggc
    19621 acagatttcg ctttatcttt tttacggcga cacggcccat ttaattgcag atttcgttac
    19681 cacgacgaca atgaccaatc caggagagtt aaagtatgtc cgctgaacac gtactgacga
    19741 tgctgaacga gcacgaagtg aagtttgttg atttgcgctt caccgatacc aaaggtaaag
    19801 aacagcacgt cactatccct gctcatcagg tgaatgccga attcttcgaa gaaggcaaaa
    19861 tgtttgacgg ctcctcgatt ggcggctgga aaggcattaa cgaatccgac atggtgctga
    19921 tgccagacgc atccaccgca gtgattgacc cgttcttcgc cgattctacc ctgattatcc
    19981 gttgcgacat ccttgaacct ggcaccctgc aaggctatga ccgtgacccg cgctctattg
    20041 cgaagcgcgc tgaagattac ctgcgttcta ctggcattgc cgacaccgta ctgttcgggc
    20101 cagaacctga attcttcctg ttcgatgaca tccgtttcgg atcgtctatc tccggttccc
    20161 acgttgctat cgatgatatc gaaggtgcat ggaactcctc cacccaatat gaaggtggta
    20221 acaaaggtca ccgtccggca gtgaaaggcg gttacttccc ggttccgccg gtagactcag
    20281 ctcaggatat ccgttctgaa atgtgcctgg tgatggaaca gatgggtctg gtggttgaag
    20341 ctcatcacca cgaagtcgct accgcaggtc agaacgaagt ggctacccgc ttcaatacca
    20401 tgaccaaaaa agctgacgaa attcagatct acaaatatgt cgtgcacaac gtggcgcatc
    20461 gtttcggtaa gaccgcgacc tttatgccaa aaccgatgtt cggtgataac ggttccggta
    20521 tgcactgcca catgtcgctg tctaaaaacg gcgtcaacct gtttgctggc gacaaatacg
    20581 caggtctgtc tgaacaggcg ctgtactaca ttggcggcgt aatcaaacac gcgaaagcga
    20641 ttaacgccct ggcaaacccg accaccaact cttacaagcg tctggtcccg ggctacgaag
    20701 caccggtaat gctggcttac tctgcgcgta accgttctgc gtctatccgt attccggtgg
    20761 tttcttctcc gaaagcacgt cgtatcgaag tacgtttccc ggacccggcg gctaacccgt
    20821 acctgtgctt tgctgccctg ctgatggccg gtcttgatgg catcaagaac aagatccatc
    20881 cgggcgaagc catggacaaa aacctgtatg acctgccgcc agaagaagcg aaagagatcc
    20941 cacaggttgc aggctctctg gaagaagcac tgaacgaact ggatctggac cgcgagttcc
    21001 tgaaagctgg tggcgtgttc acggatgaag cgatcgatgc gtacatcgct ctgcgtcgcg
    21061 aagaagatga ccgcgtgcgt atgactccgc atccggtaga gtttgagctg tactacagcg
    21121 tctaatagtt gaagttgtac tacccggcgc aacaacgccg ggatttagtt gccgtggaaa
    21181 ctttcagccc atctctgcat gggctttttt ctccgtcaat tctctgatgc ttcgcgcttt
    21241 ttatccgtaa aaagctataa tgcactaaaa tggtgcaacc tgttcaggag actgctttat
    21301 ggcaacaggc acgcagcccg atgctgggca gatcctcaac tcgctgatta acagtatttt
    21361 gttaatcgat gacaacctgg cgatccatta cgccaacccc gccgcgcaac aactgctcgc
    21421 ccaaagctcc cgcaaattgt ttggtacgcc gttaccggaa ctgttgagct acttctcatt
    21481 aaatatcgag ctgatgcaag aaagtctgga ggcggggcaa ggttttaccg ataacgaagt
    21541 gacgctggtc atcgacgggc gctcgcatat cctttctgtg acggctcagc gtatgccgga
    21601 cggcatgatc ctgctggaga tggcaccgat ggataaccag cgccgcttaa gtcaggaaca
    21661 gctacagcac gcccagcagg ttgctgcccg tgatttagtg cgcggcctgg cacatgagat
    21721 taaaaatccg cttggcggtt tacgtggcgc ggcgcagcta ctcagcaagg cattacctga
    21781 cccgtcacta ctcgaatata ccaaagtgat tatcgaacag gcggaccggc tgcgaaatct
    21841 ggtcgaccgt ctgttggggc cgcagctgcc cggtacgcgt attaccgaaa gtattcacaa
    21901 agtggctgaa cgcgtggtga cgctggtgtc gatggaactg ccgaacaacg tgcggttgat
    21961 tcgggattac gaccccagcc tgccggaact ggcgcacgac ccggatcaaa ttgaacaggt
    22021 cttgctgaat attgtgcgca atgcgctaca ggcgctgggg ccggagggtg gtgaaatcat
    22081 tctgcgtacc cgcacagcgt ttcaactgac cttacacggc gagcgctatc ggctggcggc
    22141 gcggattgat gtggaagata acgggccggg cattccgccc catttgcagg atacgctgtt
    22201 ttacccgatg gtcagcggcc gcgaaggtgg caccgggctt ggcttatcca tcgcccgtaa
    22261 tttgattgat cagcattcag gcaaaattga atttaccagt tggccaggtc ataccgagtt
    22321 ctcggtttac ctgcctatca ggaaataaag gtgacgttta tgcaacgagg gatagtctgg
    22381 gtagtcgatg acgatagttc catccgttgg gtgcttgaac gtgcgctcgc tggagcgggt
    22441 ttaacctgta cgacatttga gaacggcgcg gaagtactgg aggcgctggc gagcaaaacg
    22501 ccggatgtgc tgctttcaga tatccgtatg ccgggaatgg acgggctggc gctgctcaag
    22561 cagattaaac agcgccatcc gatgcttccg gtcatcatta tgaccgcaca ttccgatctg
    22621 gatgctgccg tcagcgccta tcaacaaggg gcgtttgatt atctgcccaa accgtttgat
    22681 atcgacgaag ccgtggcgct ggttgagcgc gccatcagtc attatcagga acagcagcag
    22741 ccgcgtaatg ttcagcttaa cggcccaacg accgatatca tcggcgaagc gccagccatg
    22801 caggacgtgt tccgtattat cggtcggctt tcgcgttctt ccattagcgt gctgatcaac
    22861 ggcgaatccg gcactggtaa agaactggtc gctcatgccc tgcatcgcca cagcccgcga
    22921 gccaaagcgc cgtttatcgc gctcaatatg gcggctatcc cgaaggattt gatcgaatca
    22981 gaactgtttg gtcacgagaa aggcgcattt accggcgcga ataccattcg tcaggggcgt
    23041 tttgaacagg ctgatggcgg tacattattt ctcgatgaaa ttggcgatat gccgctggat
    23101 gtgcagacgc gtttgctgcg cgtgctggca gacggtcagt tttatcgcgt tggcggctat
    23161 gcgccggtga aagtagatgt gcggattatc gctgccactc accagaatct tgaacagcgg
    23221 gtgcaggaag gcaagttccg tgaggatctg ttccaccgcc tgaacgtcat ccgcgttcat
    23281 ctgccgccgc tgcgcgaacg tcgggaagat attccccgtc tggcgcgcca ttttttacag
    23341 gttgccgcgc gagagctggg cgtagaagcg aagttgctgc atccggaaac cgaagccgcg
    23401 ctgacgcgtc tggcgtggcc aggcaacgtg cgccagctgg aaaacacctg tcgctggcta
    23461 acggtgatgg ccgccgggca ggaagtgttg attcaggatt tgcctggcga actgtttgaa
    23521 tccaccgtag cagaaagccc ttcgcacatg cagccggaca gttgggcaac gctgttagca
    23581 cagtgggcag acagagcgct gcgttccggt catcaaaacc tgctttccga agcacagcca
    23641 gagctggaac ggacgttact gaccaccgcg ctgcgacata cgcaggggca taaacaggaa
    23701 gcggcgcggc tacttggctg gggccgcaac accctgacgc gtaagttaaa agagctgggg
    23761 atggagtgat tcacggcttg tgtgtaaaga ttgattattg agcgcaaatt gctggtattt
    23821 tgcgctttac tgttccgata agttcagtat gatcttgccc ggaaaacggg gagagtcatt
    23881 atgctggaat caataattaa tctggtatcg agtggcgcag ttgacagcca cacaccgcaa
    23941 actgctgttg ctgccgtgct gtgtgccgcg atgattggtc tgtttagctg aagctcgttg
    24001 tttacgccgg atgcggagaa agtcgtaggc ctgataagac gcaaccagcg tcgcatcagg
    24061 cgttgattgc cggatgcggc gtaaacgcct tatccggcct acaaaagcaa taatttcaac
    24121 agattttgca ggcctgataa gcgtagcgca tcaggcaatc ttgttctcag cacattgatg
    24181 gcgctttcgt tttctacttt gtaaacgaag cgccattcac aatttaaatc acccgagaga
    24241 actgctgcat ccgcgctttc tggcgcagat aggtatcaaa gcacatgcaa atgttgcgga
    24301 tcagcaagcg acctttcgcc gtcacctgta ttcccttctc atccacatcc accagcccat
    24361 cttttgctaa cggggcgagc aacttgagat cttccgcaaa gtaatcagcg aagagcaaat
    24421 cccactgttg ctcaataggg gcgtaatcca gacggaagtt gcagatgagc gacttaatca
    24481 catcgcggcg aatacagtca tcacgcgtta gcgcaatacc acgccacagc gcattgcctt
    24541 gttcatccac ttgctgatag tactgcttca actctttctg gttctgcgcg tagcagtcgc
    24601 caatcatgct gatggcggaa acgcccatcc ccagcagatc ggtatcgccc tgagtggtgt
    24661 agccctggaa gttacgatgc agcacgcctt cacgctgggc caccgccagc tcgtcatccg
    24721 gacgggcaaa gtgatccata ccgataaact gatagcccga ttgtgtcagg aaggcgatgg
    24781 tttcctgcag gatatcgagt ttttgctgcg gactcggcag gtcagcgtct ttgattttgc
    24841 gctgagcagc aaaaatggtc ggcaaatgcg cgtagttaaa gacgctcaga cggtcagggt
    24901 tcagttccgc cacacgcttc agggtaaagg cgaaactctc cggcgtctgt ttcggcaggc
    24961 cgtaaatcag gtcgatgttg gtggaggtaa aaccaatctc acgcgcatgg ttaagcagtg
    25021 caaagatgaa ctcttcatcc tgctcgcggt taaccagacg ttgcacttct ttgttgaagt
    25081 cctgcacgcc catgctcagg cgattaaagc cttcggcgcg taaatgatcg agtacatcca
    25141 gttcgatttc ccgcggatcg acttcgatcg aaatctccgc atcggcattg aactggaagt
    25201 tttcgcgcag cagcttcatc agacggctga tttgcgcttt attcagatac gtcggcgttc
    25261 cgccgcccca gtgcaattgg ctgacgtgac gcccggcgaa cagcggtgca cgatggacga
    25321 tttcttgctc cagcgcgtcc agatactgat cggccttgtg ctgctggcga gtaacaatct
    25381 tattgcaacc gcagaagtaa caaagcttat ggcagaacgg aatatgtacg tagagagata
    25441 atggacgctc aggatagcgc gccacggctt gtaaaaacgc ctgttcgccg aagtcttctg
    25501 aaaactccag cgcggtcggg tacgaggtgt atcgtggccc ggaatagtta tatttctgga
    25561 tcagggccag atcccagtcg atttgctgta cagacatgct cactccttcc gatggcgtct
    25621 ctggcggcta cggcgtaccg gcggatgccc gtttcgggtc atcagccggt tgcgcagcca
    25681 cttttgtcgc cgcgacaacc gctgtagttt aacgaataac cacaccagat aacatataac
    25741 cggaagggtt atcagcagaa cgggaaggcc cacggcggca atccgttagt tgccccgcag
    25801 cagacgcatc atgtcttctt gcttctcgtc ttcttcctct tcttcgtcat cgtcataaga
    25861 gaggccgagt ttctgcatca actcatcaat acgatccagt ttagcatcca cccaggattg
    25921 ctcttcggcg ctcagggttt cgcctgcttc cagacgttcc agcagcgcat ccagacgctc
    25981 atccgtttcc agtaactcca actccgcctg cggtgaaagc ataggtttct cactcttcgg
    26041 tttgtgctgt ttggtgactt tttcagccac gcccaatgga ataggggttt tactgccaat
    26101 acgtggatct tttggtgcgt tctggccttt gctgcctgac gtggtgttac cacccgctgc
    26161 gcggctgccc ggcgcatgac cacgacgttt tttctgacgc ttacggtcac gagcttcctg
    26221 atccagctcc tcgcgtgttt ttctacgcgc ttttgcatga cctttgctgc gtgagtttga
    26281 agatgatggt ttcataatgg ttcgtctttt agccgtttta ttcagtatag atttgcggcg
    26341 gaatctaaca gaaagcaagc aaagaaaaaa ggcgacagat tactctgtcg ccttttttcc
    26401 tgactcataa cccttaacgg gtcttacaat ccttgcaggc cgccaacaca ccctgttttt
    26461 ccattagccg gaacaatccg tgttgattcc atttccattt aattacgtct ccggacgttt
    26521 atcttcctga caatcctctg tcttcgcctc ctggcgctcc tgaccctcat cctgagtcat
    26581 tgttcctgtt agcgtcctcg ctctatgggt gctactttac gcctttgctt taaataaaca
    26641 atcaaccgcc atcagtattt gcacattttc agagttttat ataaaagcaa ttcactgatt
    26701 tatatagctt atttgacatg tgtcgcagaa ttttctctgc tatttcgcat aaagactatc
    26761 ggcaatgtct cacaaagcat atggctttta cttacaacgc tcacgccgaa aaatccgcct
    26821 tttattatgg ttcaggtata atcgccgcaa agctttgact gatggagacg accgctttga
    26881 ctaatttgaa ttatcaacag acgcattttg tgatgagtgc gcctgatatt cgccacctac
    26941 cttccgatac cggaattgaa gtggcttttg caggccgttc caacgcaggt aaatccagcg
    27001 cgctgaacac gctgactaac cagaaaagcc tggctcgcac ctcaaaaacc ccagggcgca
    27061 cccagcttat caacctgttt gaagtggctg acggcaagcg tctggttgac ttgcctgggt
    27121 acggttatgc ggaagtcccg gaagagatga agcgcaaatg gcagcgcgcg ctcggcgagt
    27181 acctcgaaaa acgtcagagc ctgcaaggtc tggtggtgct aatggatatt cgccatccgc
    27241 tgaaagattt ggatcagcag atgattgagt gggcggtaga cagcaatatc gccgtgctgg
    27301 tactgctgac caaagcggac aaactggcaa gcggcgcacg taaagcgcaa ttgaatttgg
    27361 tgcgtgaagc ggtgctggcg tttaacggtg atgtgcaggt tgaaacgttt tcttcgctga
    27421 agaaacaagg cgtggacaag ctgcggcaga aactggatac ctggtttagc gagatgcagc
    27481 ctgtagaaga aacgcaggac ggcgaataat tttcttgcct taatgcttgt gccggatgtg
    27541 gcgtatccgg cccgtaaatt caatagatta caattctacc gttggcctga taaacatggc
    27601 gtatcaggca ttacggatct tttctttcgc ccaataaaaa acgccccagt cattactgac
    27661 tggggcggct aaaatattca gccaaatccg attacgtgaa gtaaaaggtc tgaaagatag
    27721 aacatcttac ctctgtaccc tacgcgacta actttactct tttttgttca gttcagaaag
    27781 cattttttgt aatttttttt cagttgttgc ataggaattt ctaatagcca tcacaaaacg
    27841 tgacagctta tgttgcttac ttacgaaaaa agggcatgtt caggcgaatc ttagtgcgcc
    27901 tgatcccagt tttcgccact ccccacttcc accagtaacg gcacatccag acgggtacag
    27961 ttttccatca gttgatgaat ctgcttcgcg acggcatcga catcatcttt atggacttca
    28021 aataccagtt catcgtgtac ctgcatgatc atacgcacac gcggttgctc agcctgtagc
    28081 cacgcatcaa cggcaatcat cgcccgtttg ataatgtcgg cggcggttcc ctgcattggc
    28141 gcgttaatgg ctgcacgttc agccgctgca cgacgagcac cattgctgga tttgatatcc
    28201 ggcagataca gacggcgtcc gtccagcgtt tcaacgtagc cctgctcttt cgcctgagca
    28261 cgggtgcgtt ccatatactc cagcacgcca gggtagcgtt cgaagtaaag gtccatgtac
    28321 ttctgcgctt ctttacgcgg aatgttcaat tgccgcgcca gaccgaaagc actcatgcca
    28381 taaatcagac caaagttgat cgctttcgcg ctacgacgtt gctcgctggt gacggtttcc
    28441 agtggcaaac caaacacttc tgccgccgtt gcacggtgga tatcttttcc ttcggcgaat
    28501 gcggtcagca agcctttgtc acgcgagaga tgcgccataa tgcgcagttc aatctgcgag
    28561 tagtccgctg agacaatcac ataatcctct ggcgcaataa acgcctggcg gatacggcga
    28621 ccttcttcgt tacgcaccgg aatgttttgc aggttaggat cggttgacga taaacgtccc
    28681 gttgcggtta ctgcctggtg ataagaggta tgtacacgcc cggttttcgg gttgatcatc
    28741 aacggcagct tgtcggtgta ggtcgatttc agcttcgcca gaccacgata ctccagaatc
    28801 acttttggca acggatagtc cagcgccagt tcttccagta cctcttccga cgttgacggc
    28861 gcgccacccg gcgttttctt cagtggttta atgccctgtt tttcaaacag aatggtttgt
    28921 aactgcttgg tggaagaaag gttaaattct tcacctgcaa tttcatgagc tttcttttcc
    28981 agctcagcca gacgcaaggt gagctcttca gagtgattgt gcagcacttt cggatcgatc
    29041 ttcacaccgt tacgttcaat gcgtgaaagc accggcacca gcggcatttc gatattctcg
    29101 aagacgttca acggcccttt gtgtttttgc agatccggcc acattttcag atgcaactgc
    29161 aaggtgacat ctgcatcttc ggcggcgtaa cgtccggctt cttcgagggc aatctggtta
    29221 aaggtcagtt gatttttgcc tttacccgca atctcttcaa aagtgatggt tttgtgcttc
    29281 aaccaacgtt ccgcgaggct gtccatatcg tgacgtccgg caacgctatt gagaatgtag
    29341 gactccagca tggtatcaaa cgcaatccca cgtagttcaa tgccgtagtt cgccagaata
    29401 ccgcgatcgt atttcaggtt ttgcccgacc ttcagcgcct tttcatcttc cagcagcggt
    29461 tttagcaact cgagtgcacg ctcgcgagag atttgatcgg gcgcatcaag ataatcatga
    29521 gcaaccggaa tatatgccgc tacgcctggc tcgatagcaa aagaaagccc gaccaggtta
    29581 gcagagatgt tatcaaggct gtctgtttcg gtatcaaatg caaataccgg cgctttttcc
    29641 agcttcgcaa tccactcttt cagtgtttct tcatcaagga tagtgacgta gttgtcataa
    29701 gaaatcaccg ttgccgtcac ttctggcgct tcgtctgcaa cactggtttc ctgcggcctg
    29761 gctgcgggtt tcgccccttt agcctgtagc catttgcccg cttcgacatc agcagtccag
    29821 cgtttgaact catacttttt gaacagccct aacaactctt ccgccgccgg tggctgcact
    29881 tccagttgtt cacaggtcag ctccagttca acgtcggttt taatcgtcgc cagctggtat
    29941 gagagataag caacttcttt gttttgctcg agcttcgctg ccattgtttt cgcgccacgg
    30001 aagctcaacc cagcaatttt ttctggctcg gcatacagcg tatccagccc gccaagacct
    30061 tgcagcaatg cctgcgcggt tttttcaccg acgcccggta cgccaggaat gttatcagag
    30121 gagtcaccca tcagcgccag gaaatcgatg atcagttctg gcggcacgcc gtacttattc
    30181 accacctctt ccggtccgag gatggtattc gtcatggtgt tgataagcgt gatatttggt
    30241 gtcaccagct gcgccatatc tttatcgcca gtgctgatca gcaccggacg cccggctttt
    30301 tcggcttcgc gcgccagagt accaataacg tcgtccgctt ctacgccaga aaccgccagc
    30361 agcggcagtc ccatcgcttt aaccatcgcg tgcagaggtt cgatttgtgc tcgcagatcg
    30421 tccggcattg gcgggcgatg tgatttgtaa tgttcaaaca gttcatcacg aaaggttttt
    30481 cccttggcgt caaagaccac cgctgcatgc gtcggtttat attgcatgat cagactgcgc
    30541 agcatgttga ggacaccata catcgcaccg gtcggctcgc ctgcgctgtt agtcagcggg
    30601 ggaaacgcgt gatatgcgcg ataaaggtaa gatgaaccat ctacaaggat aagtggattt
    30661 tgggggatct gaaccataat gtccgtgcct gtttatcaga ttatgggtaa aggatgccac
    30721 agacaggatg aaaacatgag tttttcaccg cttttcgtga aaatcttttg tagatcttct
    30781 ggatcgctcg caaatcgtca tgtggataac tttgtgcata aattatttac atcgctttta
    30841 atttattatc atgcttttta cagcatgatg tttatctttg tttttcatgc agttaaaaat
    30901 aaagccatcc ctaataattg agatgatggg attttttcta atgtggatag atatgaatta
    30961 tttttctcct taaggatcat ccgttatttg ggtcgttttc tgctaaaatc tgcgccttgc
    31021 gcttctttag cgcactgtct atggctaacc ttttgaataa attcattatg acgagaatac
    31081 tcgctgcgat aacccttttg ctgagtatcg tattgaccat tttggtcact attttttgtt
    31141 ccgtcccgat catcattgcc gggattgtaa aactattgct acctgtgcca gtcatttggc
    31201 gaaaggtttc gcgtttttgt gattttatga tgtattgctg gtgtgaaggt ctggcggtat
    31261 tactgcatct gaacccacac ctgcaatggg aagttcacgg gctggaaggg ttaagtaaga
    31321 agaactggta tctacttatt tgtaaccacc gtagttgggc agatattgtc gtactgtgcg
    31381 tgctgtttcg taagcacatt ccgatgaata aatatttcct caagcagcag ctggcctggg
    31441 tgccttttct tggcctggcg tgctgggcgc tggatatgcc gtttatgaag cgttattccc
    31501 gcgcttattt attacgtcat ccggagcgac gtggtaaaga tgttgaaacc actcggcgtt
    31561 cttgtgaaaa gtttcgcctg catcccacca ctattgtcaa tttcgttgaa ggctcccgtt
    31621 tcacgcaaga aaaacatcag caaacccact ccacttttca aaacttgttg ccaccaaagg
    31681 cggcaggcat tgcaatggcg ctaaatgtac tgggtaaaca attcgataaa ctgcttaatg
    31741 ttacgctgtg ttatccggac aataaccgtc agccgttctt cgatatgtta agcggtaaac
    31801 tgacgcgaat tgtcgtacat gtggatttac agcctattgc cgatgagtta catggcgact
    31861 acatcaatga caaaagcttt aagcgccatt ttcagcaatg gctgaactca ttgtggcagg
    31921 aaaaagaccg gcttctgaca tcgttgatgt catcacagcg gcaggaaaag taagttttac
    31981 tcgcctctga atgagcagag gcgagtgagt attttaatga acaaaacgtc cggcacgaga
    32041 cataaattct tcttcgctca tctcctgacc gttaaaaaaa actttacccg gtgtataacg
    32101 cagttgcagc gaggcggtat tgttgtcaat cgtgattaac tgcaacatct gacctaatgt
    32161 catcatcccg ctgatttgtt tatcagcccg cttttgcgct ttttccgcat ccatcccttc
    32221 agataaatta agctgttttg cggtttctgt ggcgacatta agcggtaact tcatatcaaa
    32281 attgagcgat ttaatatctt tgtttgtgga tgatgaagat ttggctgggt cggcaatact
    32341 gatatccaga ttggcattta gttcgccgag tgcgttcttc catcttaccg gttgtttaat
    32401 ggtcggctca cttttttgta ataacggtaa atactctttg aacaatgcag cattcacttc
    32461 ttgcagagca acttcatcgt ttgctaactc agggtgtgca gcaagctgct tctgcatcgc
    32521 aatgttatac tggatgataa attggcgcac cgctgaggga tcaatagatt cagcaatcaa
    32581 tgaccactca ccactaccga gttgctgatt cccttttgtt aacttatcaa gttcgtaagt
    32641 cagattaaca ttgatataat ctttgtcttt aacgcgatcc agcctggttt ttgcaacgaa
    32701 agcatcgatt tgggcaacga cttccccctg gttgataata ttaacctgat taaatttcga
    32761 ctcgctttca ccaaccggaa atttcttttc ttctaatcgt gtcagagaat caatggtaaa
    32821 acttttcgcc gttactttgt tcattggtga aaattgcaga acaatatctt cgactttgcc
    32881 ttcaatatcc aggttagaca atgaactatc atcaccatta aaagtaaact ggccttcacc
    32941 aaaagaaaac tttccatact ctgcaggggc aacatctaat gtcgtggaga acgagccacc
    33001 aaacgcgaat cgcaatgtcg cttccacggg cgatttattt ttcgcggcga taaatagcgg
    33061 ttgtgtcagc tcattattca ctaagttcac ttttgccgcg gccagtgctg ggatgacatt
    33121 accatgcatt aacattgtga tgggcaacgg accgtgctca acatccacat caaatacaat
    33181 tttctggcct ggcttgatat tgagatcggg tgcaccgtta tcaaatgtca tttgcatttg
    33241 aaaacgggaa ttaaaaaagc ctttgtcaaa atttttataa cttaacgtca tatcgtaggc
    33301 atgttcacct ttctttttag catcgttaaa acctttaata aacttttcga caccaggctg
    33361 aatttgcgta cctgtgtacc atgtgccacc accccagatt acggctaacg caacaatggc
    33421 acctgtagct gacttacgta tcatataaat attccctgat aaaacagcaa aactttgaat
    33481 atccacttat gctgaaataa tg
//
LOCUS       DAZFJL010000037        33202 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFJL010000037 DAZFJL010000000
VERSION     DAZFJL010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.037"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..3084
                     /gene="yfaL"
                     /locus_tag="ACHJ8C_001770"
     CDS             <1..3084
                     /gene="yfaL"
                     /locus_tag="ACHJ8C_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO5551636.1"
                     /translation="INNTSFTNNYAEGYGGAIYTSSTTSPYLIDISVDDNYDQNNGVM
                     IDENNSASGYDHSATAAAGGFMYIGHSVAEFNIAADKTLVIGNTSNDGAIDSLAGTGV
                     IVKEGAGELVLNADNNAFTGEMSIQNGEVTLGRSDELMNVGDTHCQSDPQDCFGLMVG
                     STVHSEYQAELNVGNTQQTFVHSLTGFANGILNIDAGGNVTVNQGGFSGSIQGEGQLT
                     VAQDGSYLLTGAQSMALTGDIVVEDNAVLSLAGNQADLRAMQSDPQSIVLNGGVLDLS
                     DFTTWDGDSSYNDGLQISGSGGTVIGSNDVVDISSGDDLHIGGSDASQNGVYVVINAG
                     DQRVTLANNNGYLGNTQIASGTLEVSDNSQLGDTSYNRSVIFTDPQQHSEMDVTTDVD
                     TRSATTGQGRNIEMRADGEIHVEDGVDTQWGGLMADSTGQQLDSVSTLTKSGGGTLEL
                     TASGTATSAVRVEDGTLKGEAENIIPYVSSLWVGEDGVFETGQNQDIRSIDATSGGDI
                     DITDGTVLRLTQQDTNQALDASLFSGDGTLVNATDGVTLAGELNTNLETDSLTYLSDV
                     TVNGNLTNTSGAVSLQNGVAGNTLTVNGDYTGGGTLLLDSELNGDDSASDQLVLNGNT
                     AGNTAVVINPITGIGEPTSTGIKVVDFAADPAQFQNNAQFSLAGSGYVNMGAYDYTLV
                     EDNNDWYLRSQEVNPTPPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYFN
                     NLRAANQAFVMERRDHAGGDGQTLNLRVIGGRYHYTAVGQLAQHEDTSTVQLSGNLFS
                     GHWGDDGEWMLGIVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGA
                     WLDSWLQYVWFNNDVSEQDDGVDHYHSSGIIASLEAGYQWLPGHGVVIEPQTQVIYQG
                     VHQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEED
                     GSTISDDAVKQRGEIKVGITGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(3224..3946)
                     /gene="ubiG"
                     /locus_tag="ACHJ8C_001771"
     CDS             complement(3224..3946)
                     /gene="ubiG"
                     /locus_tag="ACHJ8C_001771"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO5551637.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4093..6720
                     /gene="gyrA"
                     /locus_tag="ACHJ8C_001772"
     CDS             4093..6720
                     /gene="gyrA"
                     /locus_tag="ACHJ8C_001772"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO5551638.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTSEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6869..8557
                     /gene="yfaA"
                     /locus_tag="ACHJ8C_001773"
     CDS             6869..8557
                     /gene="yfaA"
                     /locus_tag="ACHJ8C_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO5551639.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYSGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNVLMFATYQDKILAFSSSDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRQRIVVSARLLGFGYQRLVP
                     SFAGMRFEMGNDGWHSFLALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGGAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQALNKTRPAMVDVIPTDGIVPLYINPQGMAKLLRNETLT
                     SLPKNLDPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            8554..9177
                     /gene="yfaT"
                     /locus_tag="ACHJ8C_001774"
     CDS             8554..9177
                     /gene="yfaT"
                     /locus_tag="ACHJ8C_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFO5551640.1"
                     /translation="MRHGLLVLICWLYCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANEALKVHDSKWLKSNGLSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYIIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9201..12200
                     /locus_tag="ACHJ8C_001775"
     CDS             9201..12200
                     /locus_tag="ACHJ8C_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HFO5551641.1"
                     /translation="MGTGLANADDSLPSSNYVPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGKQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSVILWTDGLGVMTRGVTDGSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGRAYLRSEKSLYM
                     KWSMPTVYRMGDKPAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQN
                     IQQSGLLSAELQQNGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGDNALTLPEQASNI
                     RLQSSETPQEIFRNNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQM
                     IQDNRLRLMQLAGPGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHM
                     LDSYAEQADNMPLLHRALVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTSD
                     INDSLILDTPESPLADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHT
                     VVLLNSGGDATQAAAILSGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKH
                     KLTGGGEYWRWVGQGVPDILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRL
                     IPGEEEMSFTLQPVTSNEIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTT
                     WGISVNKPNAGKQQGQLLEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLP
                     PARYVRSYAPAQQSVAAGSEWTGMQVK"
     gene            12201..13850
                     /gene="yfaQ"
                     /locus_tag="ACHJ8C_001776"
     CDS             12201..13850
                     /gene="yfaQ"
                     /locus_tag="ACHJ8C_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO5551642.1"
                     /translation="MNWRRIFWLLALVTLPTLAEETPLQLVLRGAQHDQLYQMSSSGV
                     TKVSALPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADMWGQYWQQRQAPAWLASLPTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATALNRVLPVPTQVASGQCVLVDLFARYPLKNVTEEKST
                     TAVKPGVLNGRYRVTFANGNHMTFVSHGETTLLTVKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAEKAMTVAIRTFLQQNADREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWQQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPSGLDENYIETLTRQLLMD"
     gene            13855..14631
                     /gene="yfaP"
                     /locus_tag="ACHJ8C_001777"
     CDS             13855..14631
                     /gene="yfaP"
                     /locus_tag="ACHJ8C_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO5551643.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQTINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGAGAIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(14705..15889)
                     /gene="atoB"
                     /locus_tag="ACHJ8C_001778"
     CDS             complement(14705..15889)
                     /gene="atoB"
                     /locus_tag="ACHJ8C_001778"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFO5551644.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSTIDLGATVIKAAIERAKID
                     SLHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLNPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFE
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(15920..17242)
                     /gene="atoE"
                     /locus_tag="ACHJ8C_001779"
     CDS             complement(15920..17242)
                     /gene="atoE"
                     /locus_tag="ACHJ8C_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFO5551645.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVMPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(17239..17889)
                     /gene="atoA"
                     /locus_tag="ACHJ8C_001780"
     CDS             complement(17239..17889)
                     /gene="atoA"
                     /locus_tag="ACHJ8C_001780"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFO5551646.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(17889..18551)
                     /gene="atoD"
                     /locus_tag="ACHJ8C_001781"
     CDS             complement(17889..18551)
                     /gene="atoD"
                     /locus_tag="ACHJ8C_001781"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFO5551647.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYHLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(18747..20132)
                     /gene="atoC"
                     /locus_tag="ACHJ8C_001782"
     CDS             complement(18747..20132)
                     /gene="atoC"
                     /locus_tag="ACHJ8C_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFO5551648.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLNAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(20129..21955)
                     /gene="atoS"
                     /locus_tag="ACHJ8C_001783"
     CDS             complement(20129..21955)
                     /gene="atoS"
                     /locus_tag="ACHJ8C_001783"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFO5551649.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGNRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTRDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGSGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            22122..24971
                     /gene="rcsC"
                     /locus_tag="ACHJ8C_001784"
     CDS             22122..24971
                     /gene="rcsC"
                     /locus_tag="ACHJ8C_001784"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO5551650.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTIFDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVNKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLNKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(25171..25821)
                     /gene="rcsB"
                     /locus_tag="ACHJ8C_001785"
     CDS             complement(25171..25821)
                     /gene="rcsB"
                     /locus_tag="ACHJ8C_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO5551651.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LSKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(25838..28510)
                     /gene="rcsD"
                     /locus_tag="ACHJ8C_001786"
     CDS             complement(25838..28510)
                     /gene="rcsD"
                     /locus_tag="ACHJ8C_001786"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO5551652.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNENEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPEGKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETELFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENTQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            29249..30370
                     /gene="ompC"
                     /locus_tag="ACHJ8C_001787"
     CDS             29249..30370
                     /gene="ompC"
                     /locus_tag="ACHJ8C_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO5551653.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            30482..31537
                     /gene="apbE"
                     /locus_tag="ACHJ8C_001788"
     CDS             30482..31537
                     /gene="apbE"
                     /locus_tag="ACHJ8C_001788"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO5551654.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGAGKPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDNF
                     KTWMSPQFKSFLISEKN"
     gene            31611..32675
                     /gene="ada"
                     /locus_tag="ACHJ8C_001789"
     CDS             31611..32675
                     /gene="ada"
                     /locus_tag="ACHJ8C_001789"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO5551655.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPLQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRELLAKGESVTTSIINAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADSAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            32675..>33202
                     /gene="alkB"
                     /locus_tag="ACHJ8C_001790"
     CDS             32675..>33202
                     /gene="alkB"
                     /locus_tag="ACHJ8C_001790"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO5551656.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHHLCQR
                     AATAAGYPDFQPDACLINRYVPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVV"
BASE COUNT         8138 a         8167 c         8900 g         7997 t
ORIGIN      
        1 attaataata cctcctttac caataactat gcagaaggtt atggcggagc aatttatacc
       61 agcagcacca catccccgta tcttattgat atttctgttg atgataatta tgaccaaaac
      121 aatggtgtga tgattgatga aaataacagc gcctcgggat atgaccattc ggcgacggca
      181 gcggcggggg gctttatgta tatcggtcat agtgtggctg aatttaacat tgccgccgat
      241 aaaacgctgg taataggcaa taccagcaac gatggcgcga ttgattctct tgccggaacg
      301 ggagtcatcg tgaaagaggg agcgggagag ttagtcctca atgccgacaa taatgcgttt
      361 accggcgaga tgagtatcca gaatggcgag gtgacgttgg ggcgcagcga tgagttaatg
      421 aatgtcggcg acacgcactg tcagagcgat ccgcaggatt gctttggttt gatggtcggc
      481 agcactgttc attctgagta tcaggcagaa ctgaatgttg gcaatacaca acaaacgttt
      541 gtgcactcat taaccggttt tgctaatggc attttgaata tcgacgcagg cggcaatgtt
      601 acagtaaacc agggcggttt ttccggctcg attcagggtg aaggacagtt gacggtagcg
      661 caagatggca gctatctcct gacaggggcg cagtcgatgg cattaaccgg cgatattgtg
      721 gtggaagata acgccgtatt gtcgctggcg gggaatcagg ccgatttacg ggcaatgcag
      781 agcgatccgc aatcgatcgt gctaaatggc ggagtattgg atctctctga tttcaccacc
      841 tgggatggcg atagctcata caatgacggt ctgcaaatca gcggcagcgg ggggacagtg
      901 attggcagta atgatgtcgt tgatatcagc agtggcgatg atttacatat tggcggcagc
      961 gatgccagcc agaatggcgt ttatgtcgtc attaatgcgg gggatcagcg cgtaacgctg
     1021 gcgaataaca acggttatct cggtaatacg caaatcgcct ccggtacgct tgaagtcagc
     1081 gataattcgc aattggggga cacaagttac aaccgttcag tgatcttcac agatccccaa
     1141 caacacagtg aaatggatgt gacgactgat gttgacactc gctcggctac aacaggtcag
     1201 ggcaggaata ttgaaatgcg cgctgatggc gaaatacacg ttgaggatgg cgtagatact
     1261 caatggggcg ggttgatggc ggatagtacc gggcaacaac tggatagcgt aagcacgttg
     1321 actaaaagtg gcggcggtac gctggagttg accgccagcg gcactgcgac gtctgcggta
     1381 cgtgtggagg acggaacgct aaaaggtgaa gcggagaata ttattcctta tgtttcttca
     1441 ctgtgggtgg gggaagacgg tgtttttgaa accgggcaaa atcaggatat tcgttcaatc
     1501 gatgccacct ctggcggcga tatcgacata accgatggta ctgtgttgcg attgacgcag
     1561 caggatacga accaggcgct ggatgcctcg ctgtttagcg gcgacggtac gctggtgaat
     1621 gccaccgatg gcgtgacgct ggcaggtgag cttaatacca accttgaaac tgacagcctg
     1681 acttatcttt ccgacgtgac ggttaatggc aatctgacca atacgtccgg tgctgtcagt
     1741 ctgcaaaatg gcgtcgctgg caacacgctg acggtaaatg gtgattatac cggcggcggt
     1801 acgctactgc tcgatagcga attaaacggc gatgactcag caagtgacca actggtattg
     1861 aacggtaata ctgctggcaa cacggccgtg gtaattaacc ccattacggg tattggtgag
     1921 ccgacatcta caggtattaa agtagttgat ttcgcagctg atcccgcgca gtttcaaaac
     1981 aatgcgcagt tcagtctggc aggaagcggc tacgtcaata tgggggcgta tgactacacg
     2041 ctggtggaag ataacaacga ctggtatctg cgatcacaag aagtcaatcc gacgccgccg
     2101 cctgatccag acccgactcc agatcctgat cccacgccgg atcccgaccc aacccccgac
     2161 ccggaaccta cgcctgccta ccagccggtg ttgaatgcca aagttggtgg atattttaat
     2221 aacctgcggg cggcaaatca ggcatttgtg atggagcgac gcgatcacgc tggtggcgat
     2281 ggtcagacgc tgaatttacg cgttatcggc gggcgttatc attacacggc ggtagggcag
     2341 ttggctcagc atgaagacac ttctacggtg cagcttagcg gcaacctgtt tagcgggcac
     2401 tggggtgatg atggcgagtg gatgcttggg attgttggtg gctacagcga taaccagggc
     2461 gacagccgct cgaatatgac cggaactcgc gccgataacc agaaccacgg ttatgccgtt
     2521 gggctgacct caagctggta tcagcacggt aatcagaagc agggggcctg gttggatagc
     2581 tggctgcaat atgtgtggtt taacaatgat gtttccgaac aagacgatgg cgtggatcat
     2641 taccactcgt cggggattat cgcctcgctg gaagccggtt atcagtggtt accggggcat
     2701 ggtgtggtga ttgaaccgca gacgcaggtg atttatcagg gcgtgcatca ggatgatttt
     2761 accgccgcta accatgcgcg cgtgtcacaa tcgcagggtg atgatattca gacgcggctg
     2821 ggtttacaca gcgaatggcg tactgctgtt ggtgtcacgc caacgttaga tctgaattat
     2881 tatcacgatc cccatgcgac ggaaattgaa gaagatggca gcactatcag tgacgatgcg
     2941 gtgaagcaac ggggtgaaat aaaagtggga ataacgggca atatcagtca gcgagtttcg
     3001 ctgcgcggca gcgtggcgtg gcagaaaggg agtgatgatt ttgcccagac ggcagggttt
     3061 ttgtcgatga cagtgaaatg gtaatcgctg tctgaccggc actgttgccg gatgcggcgt
     3121 aaacgcctta tcaggcctac ggggagcatt tgtaggcctg ataagacgcg gcaagcgtcg
     3181 catcaggctt ggcgcggttg ccggatgaat ccaaacaaca acctcactta ttctgcgtgt
     3241 gcagcatata gttcacatcc acgccggggc cgagcttaaa ggtgttagtg atagggttgt
     3301 aatgcagccc ggtcatatgc cgttctttaa gactggtctg atccacccag cccagcaatt
     3361 ctgccggttt aataaacttc ttcacatcat gtgtaccttt aggcaccatg cgcaaaatat
     3421 attccgcacc aaccaccgcc atcagccatg acttgccgtt gcggttaagc gtcgagaaaa
     3481 agacatcgcc gcccggtttc accagttgcg cacaggctct gaccactgac tgaggatcgg
     3541 ggacgtgctc cagcatctcc atacaggtca ccacgtcata ctgtccggca tgttttgccg
     3601 cgtgctcttc cacggtttcc tgcacgtaat ccacctgaat gccgctttcc agtgcgtgca
     3661 gttttgccac ctgcaatggc tcaaagccca tatccagacc ggtcaccgtc gcgccctccc
     3721 gcgccatact ctcggccaga atgccgccgc cacaaccgac atcgagcacc tttttgccaa
     3781 ataaaccgcc agcacgctcg gcaatatagc ccagacgcag cgggttaatg cggtgcagcg
     3841 gtttgaactc accttcaaga tcccaccagc gggaggcgac ggcttcaaat ttagcgatct
     3901 cttcgtggtc tacgttgtgg tttaccggcg atttttcggc attcattggc acttctactc
     3961 cgtaattggc aagacaaacg agtatatcag gcattggatg tgaataaagc gtataggttt
     4021 acctcaaact gcgcggctgt gttataattt gcgacctttg aatccgggat acagtagagg
     4081 gatagcggtt agatgagcga ccttgcgaga gaaattacac cggtcaacat tgaggaagag
     4141 ctgaagagct cctatctgga ttatgcgatg tcggtcattg ttggccgtgc gctgccagat
     4201 gtccgagatg gcctgaagcc ggtacaccgt cgcgtacttt acgccatgaa cgtactaggc
     4261 aatgactgga acaaagccta taaaaaatct gcccgtgtcg ttggtgacgt aatcggtaaa
     4321 taccatcccc atggtgactc ggcggtttat gacacgatcg tccgtatggc gcagccattc
     4381 tcgctgcgtt acatgctggt agacggtcag ggtaacttcg gttccatcga cggcgactct
     4441 gcggcggcaa tgcgttatac ggaaatccgt ctggcgaaaa ttgcccatga actgatggcc
     4501 gatctcgaaa aagagacggt cgatttcgtt gataactatg acggtacgga aaaaattccg
     4561 gacgtcatgc caaccaaaat tcctaacctg ctggtgaacg gttcttccgg tatcgccgta
     4621 ggtatggcaa ccaacatccc gccgcacaac ctgacggaag tcatcaacgg ttgtctggcg
     4681 tatatcgatg atgaagacat cagcattgaa gggctgatgg aacacatccc ggggccggac
     4741 ttcccgacgg cggcaatcat taacggtcgt cgcggtattg aagaagctta ccgtaccggt
     4801 cgcggcaagg tatatatccg cgcccgtgcc gaagtagaag ttgacgccaa aaccggacgt
     4861 gaaaccatta tcgtccacga aattccgtat caggtaaaca aagcgcgcct gatcgagaag
     4921 attgcggaac tggtaaaaga aaaacgcgtg gaaggcatca gcgcgctgcg tgacgagtct
     4981 gacaaagacg gtatgcgcat cgtgattgaa gtgaaacgcg atgcggtcgg tgaagttgtg
     5041 ctcaacaacc tctactccca gacccagttg caggtttctt tcggtatcaa catggtggca
     5101 ttgcaccatg gtcagccgaa gatcatgaac ctgaaagaca tcatcgcggc gtttgttcgt
     5161 caccgccgtg aagtagtgac ccgtcgtact attttcgaac tgcgtaaagc tcgcgatcgt
     5221 gctcatatcc ttgaagcatt agccgtggcg ctggcgaaca tcgacccgat catcgaactg
     5281 atccgtcatg cgccgacgcc tgcagaagcg aaaaccgcgc tggttgcaaa tccgtggcag
     5341 ctgggcaacg ttgctgcgat gctggaacgt gcgggcgacg atgctgcgcg tccggaatgg
     5401 ctggagccag agttcggcgt gcgtgatggt ctgtactacc tgaccgaaca gcaagctcag
     5461 gcgattctgg atctgcgtct gcagaaactg accggccttg agcacgaaaa actgctcgac
     5521 gaatacaaag agctgctgga tcagattgcg gaactgttgc gtattcttgg tagcgccgat
     5581 cgtctgatgg aagtgatccg tgaagagctg gagctggttc gtgaacagtt cggtgacaaa
     5641 cgtcgtaccg aaatcaccgc taacagcgcc gacatcaacc tggaagatct gatcacccag
     5701 gaagatgtgg tggtcacgct ttctcaccag ggttacgtga agtatcagcc gctttctgaa
     5761 tatgaagcgc agcgtcgtgg cgggaaaggt aaatctgccg cacgtattaa agaagaagac
     5821 tttatcgacc gactgctggt ggcgaacact cacgaccata ttctgtgctt ctccagccgt
     5881 ggccgcgtct attcgatgaa ggtctaccag ttgccagaag ctactcgtgg cgcgcgtggt
     5941 cgtccgatcg tcaacctgct gccgctggaa caggacgaac gtatcaccgc gatcctgccg
     6001 gtgaccgagt ttgaagaagg cgtgaaagtc ttcatggcga ccgctaacgg tacggtgaag
     6061 aaaaccgtcc tcaccgagtt caaccgtctg cgtaccgccg gtaaagtggc gatcaaactg
     6121 gttgaaggcg atgagctgat cggcgttgac ctgaccagcg gcgaagacga agtaatgctg
     6181 ttctccgctg aaggtaaagt ggtgcgcttt aaagagtctt ctgtccgtgc gatgggctgc
     6241 aacaccaccg gtgttcgtgg tattcgttta ggcgaaggcg ataaagtcgt ctctctgatc
     6301 gtgcctcgtg gcgatggcgc aatcctcacc gcaacgcaga acggttatgg taaacgtacc
     6361 gcagtggcgg aatacccaac caagtcgcgt gcgacgaaag gggttatctc catcaaggtt
     6421 accgaacgta acggtttagt ggttggcgcg gtgcaggtag atgactgcga ccagatcatg
     6481 atgatcaccg atgccggtac gctggtacgt actcgcgttt cggaaatcag catcgtaggc
     6541 cgtaacaccc agggcgtaat ccttatccgt acttcggaag atgaaaacgt ggtgggtctg
     6601 caacgtgttg ctgaaccggt tgacgaggaa gatctggata ccatcgacgg cagtgccgcg
     6661 gaaggggacg atgaaatcgc tccggaagtg gacgttgacg acgagccaga agaagaataa
     6721 tttcatcgct tcatgccaaa agggagccat ctcccttttt tgaattgaaa agtccaggct
     6781 gtaaagtctg ggcttttgtc gtattagggc gcggtaaagt ttggctgtgc ccgtaaaaaa
     6841 tggctggcta tacacaagga atgtggcaat gagtggtgaa aaaaaggcga aaggctggcg
     6901 gttttatggc cttgttggtt ttggggcaat agcgctgcta tccgctggcg tatgggcatt
     6961 acaatactct ggcagtgggc cagaaaaaac gttgtcgcca ctggtggtgc acaacaatct
     7021 gcaaatcgat ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc
     7081 caaagatctc cttaccattc cgtttctcca cgatgttctg agcgaagatt tcgttttcta
     7141 ttatcagaat catgccgatc gtctgggcat tgaaggcagc attcgccgca ttgtctatga
     7201 acacgatctc acgctgaaag ataagctctt ttcgtcactc ttagatcagc ctgcgcaggc
     7261 ggcgctatgg cacgataaac aaggccatct ttctcattat atggtgctga tccagcgaag
     7321 tggtttaagc aagctgctgg agccattgct gtttgccgct accagcgaca gccagttaag
     7381 taaaacggaa atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg
     7441 ctataacggc aataacgtcc tgatgtttgc gacttatcag gacaaaatat tggctttttc
     7501 cagttcggat atgctgttta aagatgacca acaggatact gaagccacgg cgatcgcaag
     7561 tgatttgttg agtggtaaaa aacgctggca agccagcttt ggcctggaag agcgtactgc
     7621 cgaaaaaacg ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg ggtttggcta
     7681 ccagcgatta gtgccttctt ttgctggcat gcgcttcgaa atgggtaacg acggctggca
     7741 cagttttctg gcgttaaacg atgaatccgc cagcgtagat gccagtttcg attttacgcc
     7801 ggtatggaac agtatgcctg ccggagccag cttctgtgtg gcggtgccgt attcacacgg
     7861 tattgccgaa gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt
     7921 agacggtggc gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cactgtttgt
     7981 tggtcagttt gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa
     8041 tattggtgcg cacgaaagca aagcgccaga aggtgttttg ccggtaagcc agactcagca
     8101 gggcgaagcg caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc
     8161 gcaggcggcg caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat
     8221 gcaaaacaaa acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaagc
     8281 actgaataaa acccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct
     8341 ctatatcaat ccacaaggaa tggcgaaact gctgcgtaac gaaacgctga ccagtctgcc
     8401 gaagaatctc gatccggttt tttataacgc cgcacaaact ttattaatgc cgaagctgga
     8461 cgctttatct caacaaccgc gttatgtcat gaagctggcc cagatggaac ccggtgccgc
     8521 ctggcagtgg ctacccataa cctggcaacc gctatgagac acgggctgct ggtgctgatt
     8581 tgctggctgt attgtgttgt tgcccatagt gaaatgctaa atgtcgaaca atccgggctg
     8641 tttcgcgcgt ggtttgtgcg cattgcacag gaacagctcc gccaggggcc aagcccacgc
     8701 tggtatcaac aggattgcgc gggcctggtg cgatttgcgg cgaacgaggc gctgaaagtt
     8761 cacgacagta aatggctaaa aagtaacggt ttatctaacc agtatttgcc gccagagatg
     8821 acgctaacac ctgaacagcg tcaactggcg caaaactgga atcaggggaa tgggaaaacc
     8881 ggcccctatg tgaccgcgat taatttgatt cagtacaaca gccagtttat tggccaggac
     8941 ataaaccagg cactgcctgg cgatatgatt tttttcgatc agggcgatgc ccagcactta
     9001 atggtctgga tggggcgtta catcatctac cacaccggaa gcgccacgaa aactgacaac
     9061 ggaatgcgcg cagtcagtct gcaacaactt atgacatgga aggacacccg atggataccc
     9121 aacgattcca atcccaattt cattggcatt tatcgtttaa attttctggc gcgatagcag
     9181 cgtgcttgtc cctgtctctt gtgggaacag ggcttgctaa tgctgatgat tcgcttcctt
     9241 ccagtaacta tgtgccgccc gccgggggaa cattcttttt gctcgctgac agcagtttta
     9301 gcagcagtga agaggcgaaa gtgcgactgg aagcgccggg gcgtgattat cggcgctatc
     9361 agatggaaga gtacggcggc gtggacgttc gcctgtatcg tattcctgac ccgatggcat
     9421 ttttgcgcca gcagaaaaac ctgcatcgca ttgtggtgca accgcaatat ctgggcgacg
     9481 gactgaacaa tacgctgacc tggctgtggg ataactggta cggcaaatct cgccgcgtga
     9541 tgcagcgtac tttctcttct cagtcacggc agaatgtgac tcaggcgtta cccgaattac
     9601 agctcggtaa tgccattatt aaaccttccc gttatgtaca gaacaaccag ttttcaccgc
     9661 tgaaaaaata tccactagtg gaacagttcc gttacccact atggcaggct aaaccggtcg
     9721 agccgcaaca aggggtaaaa ctggaaggcg catccagcaa tttcatttcg ccgcagccgg
     9781 gcaacatcta tattcctctc ggcaaacaag agccgggact gtacctcgtc gaggcgatgg
     9841 tgggtgggta tcgggcgacg acggtggtgt ttgtttccga taccgtggcg cttagcaaag
     9901 tgtcaggtaa cgagcttctg gtgtggaccg cggggaaaaa acagggtgaa gcgaagcccg
     9961 gctcagtgat cttgtggact gacggtcttg gcgtgatgac ccgcggtgtg accgatggca
    10021 gcggtacctt gcagttacaa catatatcgc cagaacgttc atatattctg ggtaaggatg
    10081 ctgaaggcgg cgtttttgtc tccgagaact ttttctacga aagcgaaatc tacaacaccc
    10141 gcttgtatat ctttaccgat cgcccgctat atcgcgcagg cgatcgtgtc gatgttaaag
    10201 tgatgggccg cgagttccac gatccgttgc attcatcccc catcgtcagc gcaccggcga
    10261 agctttcggt gctggacgcc aacggcagtc tgttgcaaac cgtcaatgtc acgctggatg
    10321 cgcgtaatgg cgggcagggg cgtgcttatc tgcgttcgga aaaaagtctc tacatgaaat
    10381 ggagtatgcc aacggtgtat cgcatgggcg acaaaccggc agcaggactg tttatcttca
    10441 gtcagcagga taacgaaccg gtggcgctgg tgactaaatt tgcaggcgct gagatgcgcc
    10501 agacgctgac gctgcacaaa ggggcgaatt atatttcgct gacgcagaat attcagcaat
    10561 ctggcttgtt aagtgcagaa ctgcaacaaa atgggcaagt acaggacagc attagcacaa
    10621 aactgtcttt tgtggataac agctggcccg ttgaacaaca gaaaaacgtc atgctcggcg
    10681 gtggcgataa tgcgctgacg ttgcccgagc aggcgagcaa tatccggcta caaagtagcg
    10741 aaacgccgca ggagattttc cgtaacaatc ttgatgcatt agtcgatgaa ccgtggggtg
    10801 gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt tcgcttgccg
    10861 atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac cgtctgcggc
    10921 tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat ggcaatggcg
    10981 acgccttcct cacggcatgg gcatggtacg ccgactggca ggccagccag gcgctcggcg
    11041 taacgcagca accggaatac tggcaacata tgctcgacag ttatgccgag caggcggaca
    11101 acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg aatctgccgt
    11161 gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact aaaactgaag
    11221 atttctctga ggaagacacc agcgatatca atgacagcct gatcctcgat acacctgaat
    11281 ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa aaagcgcagt
    11341 tgaagtccac tgtgatgcca caggttcagc aatatgcgtg ggataaagca gcaaacagca
    11401 accaaccgct ggcgcacacg gttgtgctgc ttaatagtgg aggcgacgct acccaggcgg
    11461 ccgctatttt aagtggttta accgctgagc aatccaccat tgagcgcgcg ttggccatga
    11521 actggctggc gaaatatatg gcgacaatgc ctccagttgt gttgcctgcg cctgcgggcg
    11581 catgggccaa acataagtta accggaggag gcgaatactg gcgttgggtt ggtcagggcg
    11641 tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag gtccgctggc
    11701 gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa cgccagttgt
    11761 atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg accagcaatg
    11821 agattgacag cgatgcgctg tatctcgatg aaatcacgct gaccagcgag caggatgccg
    11881 ttctgcgcta cggtcaggtg gaagtaccgc tcccgccagg ggctgacgtt gagcggacaa
    11941 cgtggggcat ttcggtcaat aaacctaacg ctggaaaaca gcaggggcaa ttgctggaaa
    12001 aagcgcgcaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa ctgacgggaa
    12061 cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt ctgcctcctg
    12121 ctcgttatgt gcgttcctat gcacctgcac agcaaagtgt tgcggcaggg agtgaatgga
    12181 ccgggatgca ggtgaaataa gtgaactggc gcagaatctt ctggctattg gcgttggtta
    12241 ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt gcgcagcacg
    12301 atcaactgta tcagatgtcg tcatcaggcg tgaccaaagt ctcagcatta cctgacacgc
    12361 tcaccacgcc gttagggagt ttatggaaac tgtatgttta cgcctggctg gaggataccc
    12421 atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt tattgttgtc
    12481 aggctgggga gagcataacg cgcgataccg cgctggtacg ctcctgtgga ctctattttg
    12541 cgccgcaacg gttgcatatt ggtgcagata tgtgggggca atactggcaa cagcgtcagg
    12601 cacctgcgtg gttagcgtct cttccgacgc tgaaaccaga aacctcggta acggttaaat
    12661 cgctgcttga ttcattagcg acgttacccg cgcaaaacaa agctcaggaa gtcttactgg
    12721 atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt cgggtacggg
    12781 tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgtcagggt ggttttgctg
    12841 gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagt aaaacggtac
    12901 tgacccgtta tgcgacggcg cttaatcggg ttttgccagt gccgacgcaa gttgccagtg
    12961 ggcaatgtgt gctggtggat ctgtttgctc gttatccgct gaagaatgtt actgaggaaa
    13021 agagtacgac ggcagtcaaa ccaggcgtgc tcaatgggcg ctatcgtgtg acgtttgcca
    13081 atggtaacca catgacattt gtcagccacg gtgagacaac gttactgacg gtaaagggca
    13141 aactgaaatt acagtcgcat ctcgatcgtg aagagtatgt tgccagagtt ttggatcgcg
    13201 aggcgaaaag cacgcctcct gaagcagaga aagccatgac cgtggcgatt cgcacgtttt
    13261 tacagcaaaa cgccgatcgg gaaggcgatt gcctgaccat cccggacagc agcgctacgc
    13321 agcgtgtttc tgcttcgcca gctaccacgg gggcgcgaac gatgaccgcc tggacgcagg
    13381 atctcatcta tgcgggcgat ccggttcatt atcatggcag tcgcgctacc gaagggacgc
    13441 tttcatggca gcaagccacg gctcaggcgg ggcagggaga gcgttacgac cagatactcg
    13501 cgtttgctta tcccgacaac agccttagcc gctggggagc gccacgctca acctgccagt
    13561 tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg cgtatattac
    13621 aaggtgagac gggatacaac gaaccagacg tgtttgcggt ctgtcgttta gtctcaggtt
    13681 tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt acgcttcagg
    13741 atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat ccctccgggc
    13801 ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa tgctatgcga
    13861 aagatttttc ttccgttgtt actggtggcg ctttcgcctg tcgctcacag tgaaggtgtg
    13921 caggaagtcg agattgatgc accgctctct ggctggcatc cggcagaggg tgaagatgcc
    13981 agtttttcac aaacgatcaa ttacccggcg tcgtccgtca acatggcaga tgatcaaaat
    14041 atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa agttcagcag
    14101 ggtcggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatcaga tggttctttt
    14161 gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag cccagatggg
    14221 caaagccgac aaaaaatgca gttttactca acgccgggtg ctggggcgat tcgcgcacgt
    14281 ttacggctgg ttctttcgtg ggatacggac aataccgacc tcgatcttca tgtcgttacg
    14341 cctgacggtg aacatgcctg gtacggtaac accgtgctga aaaacagtgg cgcactggat
    14401 atggatgtca ctacagggta cggacccgag attttcgcca tgccagcgcc agttcacggc
    14461 cgttatcagg tgtatatcaa ctactatggc ggacgcagcg aaacggaatt aaccaccgcc
    14521 caactgacgc tgatcaccga tgaagggtcg gtcaatgaga aacaggaaac atttattgta
    14581 ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg attaatgggg
    14641 ttatctttac tgtcaatgtt caagaaaacg cccgatgact ttcgctatcg ggcgttttta
    14701 catgttaatt caatcgttca atcaccatcg caattccctg gccgccacca atgcacagtg
    14761 ttgccagccc cagcgtttta tcgcgtgcct gcattgcatg taatagtgtg accagaatac
    14821 gagcaccact ggcaccgata ggatgcccca gcgcgatggc cccgccgttg acattcactt
    14881 tctcaggctc aaagcccagg gttttcccaa cggcaaggaa ctgtgcagca aatgcttcat
    14941 tagcctcaat gagatcaata tccgccagtt gcagccccgc cagttgtaac gctttttgcg
    15001 tggcaggtac tggccccata cccatcaatg cggggggcac gccaccgctg gcataacttt
    15061 taatgcgtgc caggggattc aggcctgctg ccagcgccgc agattcttcc ataatcacca
    15121 gagcggcagc accgtcgtta atacctgacg cgttcccagc ggtgactgtt cctgctttat
    15181 cgaaggccgg gcgcaatgcg cctaacgctt cagccgtaga atccgctttc gggaattcgt
    15241 cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg atttcggctg
    15301 taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc gccagttcat
    15361 cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta atccccatat
    15421 gataaccatg ggtggcgcac atcagaccat cgcgcaggat tacgtcataa acctgtccgt
    15481 ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct aaactcatat
    15541 tttccatacc ccccgccaca atgctctgcg cctgacctgc ctgaattgcc tgggcggcaa
    15601 gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg cacactgttt
    15661 cggccagtcc gctttttaac agtgcctgac gcgccggatt ttgccccagc ccggcttgta
    15721 gcacgttacc cataatcact tcatcaacgt gtagtgaatc gatttttgca cgttcaatgg
    15781 cggctttaat tactgtcgcc cccaggtcga tggtgctggt ggaagcgagt gaaccgttaa
    15841 aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt ttatattcct
    15901 tctgtctgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg acgccggaga
    15961 agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg gcgattgcca
    16021 gtgctggcag cgcccagaac ggttgtgcca tgttcatcca ttgctcgccg taggcgatcg
    16081 ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggcatc acgaacggcc
    16141 cctgaataac ccagtgcccg ccgccagacg gaacggcgaa gttaatcagt gcagaactaa
    16201 aaaaggtcat gaccgggaag gtgtctttgt tcgcaacatt gatgaagaat tcggtaatga
    16261 gtccgcccag accggagtgc tccatcatca gttggatccc cgcgtagaag gggaattgca
    16321 ccagaatacc ggcggtactg cgtgctgccg cgctgatagc acgcatataa gccattggcg
    16381 ttttatgtag cagcagaccc gcaatcataa acatcaggtt gacggtattg atggtgatgt
    16441 tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg ccgatgatca
    16501 acgtcagaat acggctttct tccagtcgct cggacggtgg agcatctttc ggtagttgct
    16561 tttgaaaatc agcctcttcc atgagtagtt tcggatcgat actcaccacg tcagacggtt
    16621 ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgccaca gtgatgaaaa
    16681 tattaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata tgctcaaccg
    16741 ggttgcccgg tgttgcagcc aacagaggca ttgagccaga gaaaccgcca ccccaggtga
    16801 gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggt actcgccggg
    16861 cgacttcacg ggcgaacatt gcaccgacaa ccaaaccaaa tccccagttg atgacacaag
    16921 caactgaacc gaagaaagta accagcatga cgccctgtac gggcgtcttt gcggcggagg
    16981 cggcagtacg cagcaaactt ttcaccggcg cggagctggc aagggcatga ccggtaacga
    17041 tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccgtca ccccacattt
    17101 tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat gttagcaatg
    17161 tcagcaacat ggcaaagatc agtggatcgg gaagccaccg gctgacaaaa cgcgtcataa
    17221 aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg cagcgacttc
    17281 aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg caatttcggt
    17341 gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca gcatatgcac
    17401 cgcatgttgc gcagtgagtg gcatggtgca gcggcgcaaa atttttgctg aaccatcttt
    17461 ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat ccatcgcgcc
    17521 acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg cttcttcgtc
    17581 tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta gcgcaaatga
    17641 catggcgcta tcaaacatgg ctgcaccggg taaaatgccg cacggttgtc cgccagcgtt
    17701 caccagatcg ggatgcgctg ttgtgaccgg gcctaaaccg aggaagccgt tttccgattg
    17761 cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta aaccgattcc
    17821 taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa tacgttgttt
    17881 cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc accaggtgtg
    17941 acaatatggt caggttgcag ctcgccggtt tcgaccagtt catccggctc taccagcgtg
    18001 atatcagccg caagggctat cagggggtta aagttgcggg cgctaaggtg ataggtcagg
    18061 ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg cagcgggcgt
    18121 tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc tacgacggtg
    18181 ccgacaccag ttggggtgag aaaaccacca agtccagctc caccacagcg aatttgctcg
    18241 attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat gcgccgacct
    18301 gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc attgacgatg
    18361 agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt caggtcgcga
    18421 acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc cataaatccg
    18481 cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa tgtcatcaat
    18541 tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca gggaatagca
    18601 aggggagtgc cagaatgaga ttattgaatg ttgttattta actaattgaa tagaaaagga
    18661 taaattagat ggggattcat aaggagaatg agcggctatg cagaaaattg cgcactgtgc
    18721 aaatttctgc atagcaagtt ttggcgttat acatccgccg gatcgatacc gtattcctgg
    18781 agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg ggttcggttt
    18841 ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt aatttcctct
    18901 tttaaattac gctcaccgac aggggctgtt tttgcttcac cagcattaca gactggctga
    18961 cgaatctgcg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat cacgacggcg
    19021 cgttcaataa cgttggaaag ctctcgaata tttcccggcc atgaccaggc gttaagcagt
    19081 gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact gaatttttgc
    19141 aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg cggcagtatt
    19201 aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc tttcaccatt
    19261 gcctgcaagt cgcggttggt ggcagcaatg atgcggatat caacttttat ggtctgatgg
    19321 ccgccaatcc gttcaaattc ccgttcctgc agaatgcgta gtaatttggc ttgcagtacc
    19381 agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggcacg ttcaaataat
    19441 ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa cagttcactt
    19501 tcgagcaacg attccggcag tgccgcgcag ttgactttaa tgaacggccc ctttgcccgc
    19561 cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct ttcaccgcta
    19621 atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat gtccatcatc
    19681 gccgggctgt tggtgagaat gtgcccccat tgccagctgg tgctcagtgc ctggtgcaga
    19741 tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat taaattcaac
    19801 tcatcgagat caaacggttt gataacatag tcgaaggctc cgcagcgtag tgcttcgacg
    19861 gcggtttcca cttcagcata ggccgtcatc agaataacgg gtgtccgggt ctcatggctg
    19921 cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc catcaacacc
    19981 acgtcagggt gaatatcggc aaacaggtgt aatgctgtgc gcccgttgtt cgcacaatgt
    20041 gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt atcttcatca
    20101 tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc gggttgatcg
    20161 gtaaaataag cgtgaaggtt gcgccgtagc ccggcaaact ggcgacgcga atatcaccct
    20221 gatgggcatt aatgatgcgt tgacttaacg ccagaccaag cccggttcct gaggctttgg
    20281 tagtgaaaaa gggatcgaag atcttttttt gcagcgagag atcaatgcca ctgccgttgt
    20341 cctctatcga aatggcctgt tgcgagtcgc tatactgcca ggttcgaatg cgaattttcc
    20401 ctcgtgcact gatagcctgg acggcattga tcaggatatt cagtagtacc tgtttgagca
    20461 gttcacgatc ggcgttaatc gggcttaatt cattatccag ttcgcttatg aagtcgaccc
    20521 gcgcttgtac gccggcggtt tgtaccagta ccagagtttc ttcaaccaat gcattgaggc
    20581 tgacttgttg ccattgactg tggcgtggac gtgaaaattc gagcaattgc tgaataactt
    20641 tgttaattga atcgatttct ttgagtacta cggacagata ttcctgatgt attgggtcac
    20701 gggtttgttg gcgcaagatc tgtacataac cacgaatagc cgttaacgga ttacgtactt
    20761 catgcgccac gccagccatc agctcaccca gtgtggcgag gcgttctgct tgcgccatgc
    20821 ggcgctgggt ttctttgcgg gcagttaaat cagagaaaat caccaaagca cctatcattt
    20881 caccgtgcgt gttatgaata cgactggtcg tgacactgag ttcaatggtg cggtcacggc
    20941 ctggaaaact gatctccagt gctacatgtt cggtgccatg ttccagcgta tccagtacgg
    21001 gactgtagaa ctgagtattg tcgaacaaca tggagtaagg ctgccctacc agttcatgac
    21061 gttgatagcc agtgatgact tctgctgccg ggttcatggt ggttacatca ccctggcggt
    21121 caatggcaat gacgccatcg gcagcgtttt caataatcag atcgttaagt gttcgcgttt
    21181 cacgcagtgc ctgggcgagg ttgttaacac tctgactgat ttgccccatt tcaccgggca
    21241 attgtggtaa tcgagtggga atattttgtg ccagagtcga gaggccatcg gtgatgatat
    21301 ctatattggc gctcaggcga cgggagaaaa ggacaatcaa cagcaggctt atcagcaaac
    21361 cggcggtgag cacaatgata atcctcacat ccattttcca ggcctggcgg cgaatatctt
    21421 cggttaattc attggcccag atatagccga ggatttcacc attacgctca atgggaatca
    21481 ttgaattcaa aatatcgccg cgcacctgcc tgcctgaata aaccaaaggg gtatttgtac
    21541 gcatgacttc gcgaccaggg tgatctgcgg caatggtaac gcccacatta ttctgatata
    21601 gcgctgaagg cgcgtaggtg attatcgcat ccagcgtttt gttgtaataa ccagcgccga
    21661 tgccagggaa ggcgtgagtg atattttcgg taatgggggc aagttctgca tttaatgcgc
    21721 ggatacgctc ctcacgcggt aagtcgatgt agagatcgta gcgattgcct agcgcctgat
    21781 taagcaggtt gaccacggca gaaagttttt tctctttttc agataagact gctgaacgtc
    21841 cttccgtttc tacgatataa ccaatagtaa gcgttgggac aatgaccatc aggattgcca
    21901 tcaggatcat ttgattgcgt aagcggcgtg gataaatcca cttcatataa tgcatgctga
    21961 aatccactag tcttgtccgg tatatgacga ttatcagagg ttaaggtgat gatttctcgg
    22021 cggtgtatca tattccagag aagagagaac attgcggtaa cacgctttta ccgctacctt
    22081 aaccacactc catcggtcac ctgaggcgga gcttcgcccc tttgaaatac cttgcttctt
    22141 ttcgtacaac cctgaaagcc tcgcgctaca tgttcagagc attggcgtta gtgctctggc
    22201 tgttgattgc tttttcatcc gttttttaca tcgttaatgc gttacatcag cgagaatcgg
    22261 aaattcgtca ggaatttaat ctgagttccg atcaggctca gcgctttatt caacgcacct
    22321 ctgatgtgat gaaagagctg aagtacatag ctgaaaatcg cttatcggca gaaaacggtg
    22381 tgctttcccc gcgtggacga gaaacgcagg cggatgtgcc tgcgtttgaa ccgctgtttg
    22441 ccgactccga ttgttccgca atgagtaaca cctggcgagg ttctctggag tcattggcgt
    22501 ggtttatgcg ctactggcgc gataattttt ctgcggctta cgatctcaac cgggtatttt
    22561 taatcggcag cgataacctc tgcatggcca atttcggtct gcgtgatatg ccagtggaac
    22621 gcgataccgc gttaaaagct ttgcatgaac gcattaataa atatcgaaat gcaccacaag
    22681 atgatagtgg cagtaacctc tactggatca gcgaaggtcc gcgccctggc gtcgggtatt
    22741 tttacgcctt gacgccagtt tatctggcga accggttgca ggcgcttttg ggtgtcgagc
    22801 aaaccatccg gatggagaac tttttcttac ctggtacgtt gccgatgggg gttaccattt
    22861 ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc agaaagcaaa attaagggtg
    22921 atcctcgctg gatgcaggaa cgctcctggt ttggctatac ggaagggttc cgcgaactgg
    22981 tactgaaaaa aaatctgcca ccctcatcgc taagcatcgt ttattcagtg ccagttgata
    23041 aggtgctgga acgcattcgc atgttgatcc ttaacgcaat tttgctgaat gtgcttgccg
    23101 gagctgcatt gtttactctc gcgcggatgt acgagcggcg tattttcatt ccggcggaaa
    23161 gcgacgccct gcgactggaa gaacatgagc agtttaatcg caagattgtc gcctccgcgc
    23221 cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa tattttaagt aacgaactgg
    23281 cgcataccta tctcaatatg cttacgcatg aggaccgcca acggctgaca caaattatct
    23341 gtgggcagca ggtcaatttt gttgatgtcc tgaccagcaa caataccaat ctgcaaatca
    23401 gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat ttgtgtgctg gtggatgttt
    23461 cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc acaagcagcg gaacaggcga
    23521 gccagtcaaa atcgatgttc ctggccaccg tcagtcatga gctgcgaacg ccgctgtatg
    23581 gcattatcgg taacctggat ctgttgcaaa ccaaagagtt accgaaaggc gtcgatcgtc
    23641 tggtgacggc aatgaacaac tcttccagcc tgttgttgaa aattatcagc gatattctcg
    23701 atttctcgaa aattgaatca gaacagttga agatcgaacc gcgtgagttt tcaccgcgtg
    23761 aagtgatgaa ccacatcacc gccaactatt taccgctggt ggtacgcaag cagttaggct
    23821 tgtactgctt tattgaaccg gatgtgccag tggccttaaa tggcgacccg atgcgtttac
    23881 aacaggtcat ctctaacctg ctgagtaacg ccataaaatt caccgatacc ggctgtatag
    23941 ttttgcatgt tcgcgctgat ggcgattatc tctctatccg tgttcgcgat accggcgtgg
    24001 ggattccggc aaaagaagtg gtgcgtttgt ttgatccctt cttccaggtc ggaacgggcg
    24061 tacagcgtaa cttccagggg actggtctgg gtctggcgat ttgtgaaaaa ctgatcagca
    24121 tgatggacgg cgacatctcg gtagattcag aaccgggaat gggcagccag tttaccgtgc
    24181 gtattccgtt gtacggcgct cagtacccgc agaaaaaagg tgtggaaggg ttgagtggta
    24241 aacgctgctg gctggcggtc cgcaatgcgt cgctctgtca gttcctggaa accagtttgc
    24301 agcgcagcgg catcgtcgtt acaacatacg aagggcagga accgactccc gaagatgtgt
    24361 taattactga cgaggtagtg aacaaaaaat ggcagggcag agcggtagtg accttctgtc
    24421 gtcgccatat tggtattccg ctggagaaag cgccagggga gtgggtacac agtgtggctg
    24481 ctccgcatga gctaccggca ttgttggcgc gtatttattt gatcgagatg gagagcgacg
    24541 atcctgctaa cgctctgccg tcgacggaca aagcggtcag cgataatgac gatatgatga
    24601 ttctggtcgt ggatgatcat ccgattaacc ggcgtctgct ggcagatcag ttgggatcgt
    24661 taggctatca atgtaaaacc gcgaatgatg gcgtcgatgc gcttaatgta cttaacaaga
    24721 atcatattga tatcgtgctt agcgacgtca atatgccaaa tatggatggt taccgcttga
    24781 cgcaacgcat tcgtcagttg ggactgacgt tgccggtaat cggagtaact gctaatgcgt
    24841 tggctgaaga gaagcagcgg tgtctggagt ccggtatgga cagttgcctg tcgaagccgg
    24901 taacgctgga tgtgataaaa cagacgctga cggtatatgc cgagagggtc aggaaatcgc
    24961 gggaatcgta ggacggataa ggcgtttacg ccgcatccgg cattcagtgc aaatgccaga
    25021 tgcgacgctg acgcgtctta tctggcctac tttaatgtaa tctgttgaaa taatgggaat
    25081 cgtaggacgg ataaggcgtt tacgccgcat ccggcaatcg gtgcaaatgc cagataagac
    25141 actaacgcgt cttatctggc ctacaggtga ttagtcttta tctgccggac ttaacgtcac
    25201 tgaagagaga taattcagca gggcgatatc gttctcgaca cccagcttca tcatcgcaga
    25261 tttcttctgg ctactgatgg ttttaatact gcggttcagc tttttagcga tctcggtcac
    25321 caggaagcct tccgcaaaca ggcgcagaac ttcactctct tttggcgaga gacgcttgtc
    25381 accgtaacca ccagcactga ttttttccaa caggcgagaa acgctttccg gggtaaattt
    25441 cttccctttc tgcagcgcgg cgagagcctt cggcaaatcg gtcggtgcac cttgtttcag
    25501 cacgatccct tcgatatcca gatccaatac cgcactaaga atcgccgggt tgttgttcat
    25561 ggtcagaaca atgatcgaca ggcttgggaa atggcgcttg atgtacttga ttaaggtaat
    25621 gccatcgccg tacttatcgc caggcatgga gagatcggta atcaacacat gtgcatccag
    25681 tttcgacaag ttgttgatca gtgctgtaga gtcttcaaat tcgccgacaa cattcaccca
    25741 ctcaatttgc tcaagtgatt tgcgaatacc gaacaagact atcggatggt catcggcaat
    25801 aattacgttc atattgttca tgtaataggc taccttgcta cagcaagctc ttgacataac
    25861 tgtcaatgtc gctgatgtat ttttctattc ctggaacatc cttctcacga atcagatgtt
    25921 ccagcgtttc acataactgc ttgccgggta ccagatttag catggcaaat acgcctttaa
    25981 gacgatgagc cgtttgggct aacgcagcaa agtcactggt tgctgcttca gtatatagcc
    26041 tcttaacatc atccggtact gtgtctacaa agagcgcata atagccgctg gcatggagtt
    26101 gcgtattttc atctccgccc agaggcgatt ctgtcacctc ttcctgcgcc agttgcactt
    26161 caattaattg taagaccgct tcctgcatag cgttgctcat attgaaattg acgcacaatt
    26221 gaccaggccc aatttcccgt acgccagact catcatcgct taaaagcaag ccagaggcag
    26281 taagattaga cggattatcc gttaaaaaga tatcataatc ttgactaatt aatctttcat
    26341 cgggtgtgat acaggttgca ccccaatttt ctaactggcg agtgacaata ttccgaattt
    26401 ctgccgaagt aacatccacc attacgcaga catcatccag taatcgctct tcttcctctt
    26461 caacttccgg gtcagctgcg agcattttga tatgcacaga gtagcgtgta ccaagcccat
    26521 cccgcgtttt gatgtttaaa tgaccgccca gtttacgcgc cagttgatcg ctcagccaga
    26581 atgccagcgg gtccgccttg ccatagcgat cgttttgggt ctggttgata aacgggaagt
    26641 gcaaattatc catttcatga atgcttacgc cttctccagt gtccagaatg cggaacgtca
    26701 ggcggtcttc ggaggactca tcctgatcaa cctcaagggt gatttttccc aattgcgttg
    26761 aggtcacggc atattgcatc agtagtagca aaatacgtcg taaggcatcg cgatcgccgc
    26821 ggcgcatatc gtgtgctttc agatgattgt taatcagcag ttgcagacct ttacgcttga
    26881 tggcaggcaa cactgaaggc acaacttcat caattaaatc ctgcacggag aacagctccg
    26941 tctcactttt ccagctatcg tccgcaagca tgttcgctaa ctgtatttca tcgaccagcc
    27001 gcaccagcac atctgcctga ttcgccagtt gtttgccttc cggggcgttg agtttagccg
    27061 cgctctccgc cagggactgt gcgggttctt tcagcgcatc gccaatgttt ttcataaagg
    27121 tcatccgccc ctgctggttt ttctcataca gacgctgcgc ctgcttgagt ttcttgttta
    27181 ccagcacttc gcgatcctga tcgcgaataa tgaaaatttg tgtgcgcggc gcaacctggc
    27241 tgcgaaacat gcggatctca tacagctcgt tattgatcgt cgcctgaata atcccctgat
    27301 gctgttccgc catggtggtg atgttttgta gattcaaatg cggcagcaaa tgatcggcaa
    27361 ttttgttact tatgacagtg cggttcgatt cctgatcgtg aaccagcagg ccgagcggca
    27421 gcagtgagac tatctcttca ttgattgccc gtaaaatgcg caattcgtta ttgaccgccg
    27481 tgttggggac gctttctgta ctgcggctgg agaaatggcg gaatgtggta tagccaaata
    27541 acgccagcgc cagcaaaccg atgttcagca gcagtggcag cagaatgttt tgcaacgtat
    27601 ccagcagtaa ggtgccgtaa ggaacctgcc agaccaggcg catatcggta gagttgagtg
    27661 ccgaggagat ttcaatcttc gtactgttaa agtggatact gacgctatcc gtcccttctt
    27721 tctcattttc attgtttccc gttgccgtcg cgtctggctc aaggcggaaa ctgtccagcg
    27781 gcatacccgg tgggatcaaa tcattaatcg gcagatcaaa agccacgacc gttgccagat
    27841 gtcccggctg gttgaacgtg gtacgcaagg taaagtaatg accgttctgc caggccaggc
    27901 ggcgcatgtt agaaaaactt tcgcgttcat cgagggcgtt ggcctgttgt aacatctctg
    27961 cacgacgtga atcaacaatg tcgctgacgg tcgattcttt aaatccggag gtgagatctt
    28021 tgaggggcag ggttgagatc agcaccagac tgttatcctg accattcagg taatacatcg
    28081 accacggtac attttctgcg ccccacaatg tgtccagata ggtggacatc cgttgagtca
    28141 tctcaagcgt tgagttgtcg tgagagccaa atattaacgc ttccgttttg cggcgtggtt
    28201 tctccagata gtagacatcc tgtttcaggc gcgtctcttg taaaccttcg ccggaggagg
    28261 gggaggtcgt cgcggcaatg ttgtcgtaga tctgccaggt cacgtaacgc caggtatcga
    28321 cgcgcttttg gattgcgtgg gtaatgtcga caatctggta acttttatct ttcagccagg
    28381 cgttaacggc gctttgtacc attacaccca tcgtcaccag taacacaatg atcaacagta
    28441 aaaagaagcg ggtaatgctc cccggtagga gtgaaaagcg ggtcgtggcc gttgtctctt
    28501 tctgacgcat tcgtgtttat gacctgttaa aacttcgcga agtgataggg cagtataaag
    28561 ggtacagtgt gaaattccag actcttacct gattgatgat tctctattct cccgtgatat
    28621 ttatcatcct gcattggggg aaatagatgt acaagcgcca ttttgctgta caaataacct
    28681 acgaaaagcc caacgaaatt aattgcaaat aaacgaagat tgctggaaat tatgctgatg
    28741 ttaattattt gtgaaatagt taacaagagt tatagttttt ctgtggtagc acagaataat
    28801 gaaaagtgtg taaagaaggg taaaaaaaac cggatgcgag gcatccggtt gaaatagggg
    28861 taaacagaca ttcagaaatg aatgacggta ataaataaag ttaatgatga tagcggaaga
    28921 tattctagtt gcgagtgaag gttttgtttt gacattcagt gctgtcaaat aattaagaat
    28981 aagttattga ttttaacctt gaattattat tgcttgatgt taggtgctta tttcgccatt
    29041 ccacaataat cttaaaaagt tcccttgcat ttacattttg aaacatctat agcgataaat
    29101 gaaacatctt aaaagtttta gtatcatatt cgtgttggat tattctgcat ttttggggag
    29161 aatggacttg ccgactgatt aatgagggtt aatcagtatg cagtggcata aaaaagcaaa
    29221 taaaggcata taacagaggg ttaataacat gaaagttaaa gtactgtccc tcctggtccc
    29281 agctctgctg gtagcaggcg cagcaaacgc tgctgaagtt tacaacaaag acggcaacaa
    29341 attagatctg tacggtaaag tagacggcct gcactatttc tctgacgaca agtctgtaga
    29401 tggcgaccag acctacatgc gtcttggctt caaaggtgaa actcaggtta ctgaccagct
    29461 gaccggttac ggccagtggg aatatcagat ccagggcaac agcgctgaaa acgaaaacaa
    29521 ctcctggacc cgtgtggcat tcgcaggtct gaaattccag gatgtgggtt ctttcgacta
    29581 cggtcgtaac tacggcgttg tttacgacgt aacttcctgg accgacgtac tgccagaatt
    29641 cggtggtgac acctacggtt ctgacaactt catgcagcag cgtggtaacg gcttcgcaac
    29701 ctaccgtagc actgacttct ttggtctggt tgacggtctg aacttcgctg ttcagtatca
    29761 gggcaaaaac ggtagcccgg aaggtgaagg catgaccaac aacggtcgtg aagctctgcg
    29821 tcagaacggt gacggcgttg gcggttctat tacttatgat tacgaaggct tcggtatcgg
    29881 tgctgcagtt tccagctcca aacgtactga tgaccagaac ttcggtctga accgttatga
    29941 tgagcgttac atcggtaatg gcgaccgcgc tgaaacctac accggtggtc tgaaatacga
    30001 cgcgaacaac atctacctgg ctgctcagta cacccagacc tacaacgcaa ctcgcgtagg
    30061 caacttgggt tgggcgaaca aagcacagaa cttcgaagct gttgctcagt accagttcga
    30121 cttcggtctg cgtccgtccc tggcttacct gcagtctaaa ggtaaaaacc tgggtgtcat
    30181 caatggtcgt aactacgacg acgaagatat cctgaaatat gttgatgttg gtgcgaccta
    30241 ctacttcaac aaaaacatgt ccacctacgt tgactacaaa atcaacctgc tggacgacaa
    30301 ccagttcact cgtgacgctg gcatcaacac tgataacatc gtagctctgg gtctggttta
    30361 ccagttctaa tctcgattga tatcgaacaa agggcctgcg ggcccttttt tcattgtttt
    30421 cagcgtacaa acacagtttt ttggtgtact cttgcgaccg ttcgcatgag gataatcacg
    30481 tatggaaata agctttaccc gcgtggcact gctggctgcc gcgctcttct ttgttggttg
    30541 cgatcaaaaa ccacaacccg ccaaaaccca cgctactgaa gttaccgttc ttgaaggcaa
    30601 aaccatgggt accttctggc gtgccagcat tccgggcatt gacgccaaac gcagtgccga
    30661 acttaaagaa aagattcaga cccagctgga cgccgacgat cagctgcttt cgacctataa
    30721 aaaagattcc gcgctgatgc gctttaacga ctcgcaaagt ttatcgccgt ggccagtaag
    30781 tgaagcgatg gccgatatcg tcaccacctc gctacgcatt ggcgcgaaga ccgatggcgc
    30841 gatggatatt accgtcgggc cgctggtgaa tctgtggggc tttggcccag aacaacagcc
    30901 ggttcaaatt ccgagccagg aacagatcga tgcgatgaaa gccaaaaccg gcttacagca
    30961 cctgacggtc attaatcagt cgcatcagca atatctgcaa aaagacctgc cggatttata
    31021 tgtcgatctc tccaccgtcg gcgaaggtta tgcggcggat cacctggcac gcttaatgga
    31081 gcaggaaggg atttcccgct atctggtgtc ggtgggcggc gcgctgaaca gccgtggtat
    31141 gaacggtgct ggcaagccgt ggcgggtagc gattcaaaaa ccaaccgata aagaaaacgc
    31201 ggttcaggcc gtggtggata tcaacggtca tgggattagt acctctggca gctaccgtaa
    31261 ctattacgaa ctggacggca aacgtctttc tcatgttatc gatccgcaaa ccgggcgtcc
    31321 tatcgaacac aatctggtat ccgtgacggt gattgccccg acggctctgg aagccgatgc
    31381 ctgggatact ggcttaatgg tactcggacc ggagaaagcc aaagaggttg ttcgccggga
    31441 aggactggcg gtctacatga tcaccaaaga gggcgataat tttaaaacct ggatgtcgcc
    31501 gcagtttaaa agcttcctta tcagcgaaaa aaattaaagc gcaagattgt tggtttttgc
    31561 gcgatggtga ccgggcagac taacggctat ccttaaccag ggagctgatt atgaaaaacg
    31621 ccacatgttt aactgacgat caacgctggc aatctgtctt agcccgcgac ccgaacgccg
    31681 acggcgaatt cgttttcgcc gtgcgcacca cgggtatctt ttgccgtccg tcttgccgcg
    31741 ccagacatgc cttgcgggaa aatgtctcct tctacgcaaa tgccagcgag gcgcttgccg
    31801 ctggctttcg tccctgcaaa cgttgtcagc cagacaaagc caatcccctg caacatcgcc
    31861 ttgataaaat tacccacgcc tgccgacttc tggaacagga aacgcctgta acgctggaag
    31921 ccttagccga ccaggtggcg atgagtccat ttcatttgca tcggttgttt aaagcgacta
    31981 ccggaatgac gcctaaagcc tggcaacagg cctggcgcgc tcgccgtttg cgcgaattgc
    32041 tggcgaaagg ggagagcgtg actacatcta ttattaacgc cggattcccc gacagcagca
    32101 gttactaccg caaagccgac gaaacgctgg gcatgacggc taaacaattc cgtcacggtg
    32161 gcgaaaatct ggcggtgcgt tacgcgctgg ctgattgtga gctgggccgt tgcctggtgg
    32221 cagaaagtga gcgggggatt tgcgcgatat tactgggcga tgatgacgcg acactaatca
    32281 gcgagttaca gcagatgttt cccgctgccg acagcgcgcc tgccgatctg acgtttcagc
    32341 aacatgtgcg tgaagtgatc gccagcctca atcaacgcga tacgccgctg acgttaccgc
    32401 tggacattcg cggcactgct tttcagcaac aagtctggca ggcactgcgc acgatacctt
    32461 gcggtgaaac cgtcagttat cagcaactgg ctaatgccat cggcaaaccg aaagcggtac
    32521 gggccgttgc cagtgcctgt gccgccaaca agctggctat cgttatacct tgtcatcggg
    32581 tggtccgtgg tgatggcaca ctttccggtt accgctgggg cgtgtcgcgc aaagcgcaac
    32641 tgctgcgccg cgaagctgaa aatgaggaaa ggtaatgttg gatctgtttg ctgatgctga
    32701 accgtggcaa gaaccactgg cggctggagc ggtgattttg caccgttttg cttttaacgc
    32761 tgcggagcaa ctgatacgcg atattaatga cgttgccagc cagtcgccgt ttcgccagat
    32821 ggtcactcca ggcggatata ccatgtcggt ggcgatgacc aactgtgggc atctgggctg
    32881 gacgacccat cggcaaggtt atctctattc gcccattgat ccgcaaacaa ataaaccgtg
    32941 gcccgccatg ccgcagagtt ttcatcattt atgtcaacgt gcggctacgg cggcgggcta
    33001 tccagatttc cagccagatg cctgtttgat caaccgctac gttcctggcg cgaaactgtc
    33061 gctgcatcag gataaagacg aaccggatct gcgcgcgcca attgtttctg tttctctggg
    33121 cttacccgcg atttttcaat ttggcggcct gaaacgaaat gatccgctca aacgtttgtt
    33181 gttggaacat ggcgatgtgg tg
//
LOCUS       DAZFJL010000038        32960 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFJL010000038 DAZFJL010000000
VERSION     DAZFJL010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.038"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            90..1643
                     /locus_tag="ACHJ8C_001791"
     CDS             90..1643
                     /locus_tag="ACHJ8C_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFO5551657.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENIADLLHLDHLLVAECEEIDGVMNNARRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            1670..2140
                     /locus_tag="ACHJ8C_001792"
     CDS             1670..2140
                     /locus_tag="ACHJ8C_001792"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004126743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFO5551658.1"
                     /translation="MDITKILNTNRVILDMQATNKAEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVF"
     gene            2158..2472
                     /locus_tag="ACHJ8C_001793"
     CDS             2158..2472
                     /locus_tag="ACHJ8C_001793"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO5551659.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            2497..3588
                     /locus_tag="ACHJ8C_001794"
     CDS             2497..3588
                     /locus_tag="ACHJ8C_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO5551660.1"
                     /translation="MKENKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            3600..4451
                     /locus_tag="ACHJ8C_001795"
     CDS             3600..4451
                     /locus_tag="ACHJ8C_001795"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO5551661.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVALALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASAQLCVEGELGHLADAPRYDQAANADLMTQPTDVEP
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEIFHCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGAIGKA"
     gene            4532..5392
                     /locus_tag="ACHJ8C_001796"
     CDS             4532..5392
                     /locus_tag="ACHJ8C_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO5551662.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPA
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDTINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            5440..6372
                     /locus_tag="ACHJ8C_001797"
     CDS             5440..6372
                     /locus_tag="ACHJ8C_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO5551663.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            6353..7033
                     /locus_tag="ACHJ8C_001798"
     CDS             6353..7033
                     /locus_tag="ACHJ8C_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFO5551664.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CGEYGILR"
     gene            complement(7077..8786)
                     /gene="yicH"
                     /locus_tag="ACHJ8C_001799"
     CDS             complement(7077..8786)
                     /gene="yicH"
                     /locus_tag="ACHJ8C_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO5551665.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVADLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWQRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQHWGWPEL
                     PLTGDGNIQLTASGDIQVNVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(8907..10298)
                     /gene="xanP"
                     /locus_tag="ACHJ8C_001800"
     CDS             complement(8907..10298)
                     /gene="xanP"
                     /locus_tag="ACHJ8C_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO5551666.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            10578..11783
                     /gene="gltS"
                     /locus_tag="ACHJ8C_001801"
     CDS             10578..11783
                     /gene="gltS"
                     /locus_tag="ACHJ8C_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO5551667.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLILKKSMGWEVNFDMSLRDPLMLAFFATIGLNANLASLRAGGRVVGVFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(11817..13898)
                     /gene="recG"
                     /locus_tag="ACHJ8C_001802"
     CDS             complement(11817..13898)
                     /gene="recG"
                     /locus_tag="ACHJ8C_001802"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO5551668.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAVAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRIDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMTSFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(13904..14593)
                     /gene="trmH"
                     /locus_tag="ACHJ8C_001803"
     CDS             complement(13904..14593)
                     /gene="trmH"
                     /locus_tag="ACHJ8C_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO5551669.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWSTMQAAG"
     gene            complement(14600..16708)
                     /gene="spoT"
                     /locus_tag="ACHJ8C_001804"
     CDS             complement(14600..16708)
                     /gene="spoT"
                     /locus_tag="ACHJ8C_001804"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO5551670.1"
                     /translation="MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(16727..17002)
                     /gene="rpoZ"
                     /locus_tag="ACHJ8C_001805"
     CDS             complement(16727..17002)
                     /gene="rpoZ"
                     /locus_tag="ACHJ8C_001805"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO5551671.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(17057..17680)
                     /gene="gmk"
                     /locus_tag="ACHJ8C_001806"
     CDS             complement(17057..17680)
                     /gene="gmk"
                     /locus_tag="ACHJ8C_001806"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO5551672.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            17938..19620
                     /gene="ligB"
                     /locus_tag="ACHJ8C_001807"
     CDS             17938..19620
                     /gene="ligB"
                     /locus_tag="ACHJ8C_001807"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO5551673.1"
                     /translation="MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGNETRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRL
                     ISAVPQTVSGPLANSTLQGEIFLQRKGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPHLMTDRLKDLATAGFTLTQTYTRAVKNADEVAHVRNEWGKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLV
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARQQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(19617..20234)
                     /gene="yicG"
                     /locus_tag="ACHJ8C_001808"
     CDS             complement(19617..20234)
                     /gene="yicG"
                     /locus_tag="ACHJ8C_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO5551674.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIFAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(20485..21348)
                     /gene="yicC"
                     /locus_tag="ACHJ8C_001809"
     CDS             complement(20485..21348)
                     /gene="yicC"
                     /locus_tag="ACHJ8C_001809"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO5551675.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIIARETEGQALKVLIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            21475..22191
                     /gene="rph"
                     /locus_tag="ACHJ8C_001810"
     CDS             21475..22191
                     /gene="rph"
                     /locus_tag="ACHJ8C_001810"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO5551676.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            22257..22898
                     /gene="pyrE"
                     /locus_tag="ACHJ8C_001811"
     CDS             22257..22898
                     /gene="pyrE"
                     /locus_tag="ACHJ8C_001811"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO5551677.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(22935..23531)
                     /gene="slmA"
                     /locus_tag="ACHJ8C_001812"
     CDS             complement(22935..23531)
                     /gene="slmA"
                     /locus_tag="ACHJ8C_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO5551678.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QLLAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(23638..24096)
                     /gene="dut"
                     /locus_tag="ACHJ8C_001813"
     CDS             complement(23638..24096)
                     /gene="dut"
                     /locus_tag="ACHJ8C_001813"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO5551679.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(24074..25294)
                     /gene="coaBC"
                     /locus_tag="ACHJ8C_001814"
     CDS             complement(24074..25294)
                     /gene="coaBC"
                     /locus_tag="ACHJ8C_001814"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO5551680.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVNRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKALLGQLL
                     LDEIVTRYDEKNRR"
     gene            25466..26134
                     /gene="radC"
                     /locus_tag="ACHJ8C_001815"
     CDS             25466..26134
                     /gene="radC"
                     /locus_tag="ACHJ8C_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO5551681.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRIREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            26351..26587
                     /gene="rpmB"
                     /locus_tag="ACHJ8C_001816"
     CDS             26351..26587
                     /gene="rpmB"
                     /locus_tag="ACHJ8C_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO5551682.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            26608..26775
                     /gene="rpmG"
                     /locus_tag="ACHJ8C_001817"
     CDS             26608..26775
                     /gene="rpmG"
                     /locus_tag="ACHJ8C_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO5551683.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            26873..27682
                     /gene="mutM"
                     /locus_tag="ACHJ8C_001818"
     CDS             26873..27682
                     /gene="mutM"
                     /locus_tag="ACHJ8C_001818"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO5551684.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCEKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(27721..28200)
                     /gene="coaD"
                     /locus_tag="ACHJ8C_001819"
     CDS             complement(27721..28200)
                     /gene="coaD"
                     /locus_tag="ACHJ8C_001819"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO5551685.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(28208..29485)
                     /gene="waaA"
                     /locus_tag="ACHJ8C_001820"
     CDS             complement(28208..29485)
                     /gene="waaA"
                     /locus_tag="ACHJ8C_001820"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO5551686.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            29898..30956
                     /gene="rfaQ"
                     /locus_tag="ACHJ8C_001821"
     CDS             29898..30956
                     /gene="rfaQ"
                     /locus_tag="ACHJ8C_001821"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO5551687.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKITNFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRNSFTHLVPLQGGNVVESNLSVLTPLGLESLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            30953..32077
                     /gene="waaG"
                     /locus_tag="ACHJ8C_001822"
     CDS             30953..32077
                     /gene="waaG"
                     /locus_tag="ACHJ8C_001822"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO5551688.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDRVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            32070..32867
                     /gene="rfaP"
                     /locus_tag="ACHJ8C_001823"
     CDS             32070..32867
                     /gene="rfaP"
                     /locus_tag="ACHJ8C_001823"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO5551689.1"
                     /translation="MVELKEPFATLWRGKDPFGEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWAINPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQIRAKVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFGMPLREILSFERNLLNMASVKAEHIKERTQRKGL"
BASE COUNT         7913 a         8075 c         8844 g         8128 t
ORIGIN      
        1 gtgaaattat tctgctttat atttaccctc agagatggca actgaagctc ttccatatgg
       61 ggcttcttaa tctgtgataa gggtaaaaaa tgcaaatgat tacctcgcga caaaacagat
      121 tattacgatt ccttttacct cgaagggaat atacgactat tgttacgatt gccggctatt
      181 taaatgtttc ggaaaaaacc attcaacgtg atttacgttt acttgagcaa tggctgggac
      241 aatggagaat aaatgttgag aagcgtgctg gcgcgggtgt gatgttaagc gcggagaata
      301 ttgctgattt gctgcatctt gatcatttgt tggtggcaga atgtgaagag attgatggtg
      361 taatgaataa tgccaggcgc gttaaaatag cgtcgcagtt attaagtgaa acaccgaatg
      421 aaacgtcgat cagtaaattg tcagaacgct actttatcag cggagcctct attgttaatg
      481 acctgagagt aattgagtcc tggcttgcgc cgttggggtt atcgttgatc cgcagtccaa
      541 gtggtacgca tattgaaggt agtgaagggc aagtccgaca ggcaatggca ttactgatta
      601 acggcattat taaccataat gagccgcaag gtgtcgtgta ttcacgtctg gatcccggaa
      661 gctataaagc attagtccat tattttggag aagaagaggt gttatttgtc cagtcattgt
      721 tactggatat ggaaaatgaa ttaagttggt ctttgggaga accttattac gttaacattt
      781 ttactcacat ccttattatg atgtatcgca acacgcacgg gaatgcgtta tcaagagaag
      841 aagatcaaac cagacaatat gatgaaaata tctttaatgt tgccagtcag atgattcata
      901 agatagaaca acgaattgca catacattgc ccgatgatga agtctggttt atttatcaat
      961 atatcatttc atcaggtgtg gcgattgatg gacaaaaaga tgttagcatt atttcacata
     1021 tgcaggccag caatgaagcg cgtctgatta cctggcgttt aattacggta ttcagtgaca
     1081 tcgtggactg cgattttagt gaagacagcg cattatatga tggcttattg gtgcatatta
     1141 agccgctgat taaccgtcta aattatcgca ttcatatccg taatccattg ctggaagata
     1201 ttaaagcaga attagcggat gtctggcggt tgacgcaata tgtggtgaat caggtattta
     1261 aaacctgggg tgagaatgca gtgagcgagg atgaagtggg ttacctgacc gttcattttc
     1321 aggctgcgat ggagcggcaa attgcccgta aacgtgtatt actggtctgt tcaaccggaa
     1381 tcggaacttc gcatctactg aaaagccgta ttctgcgagc atttcctgaa tggacgattg
     1441 ttgatgttat ttcagcagcg aatttatcac aggttttgcc tgacaatatc gaactgatta
     1501 tttcgaccat taatttgcct acagtcacta tgccggtcgc ttatgttacc gcttttttta
     1561 atgatgccga tattaagcgg gtcactgaaa tggtgattac ggaaaaatta catcatgcga
     1621 cgtctcgggt cgttgaaatt taatgtcaga attttatgag gtatgacgaa tggacataac
     1681 aaaaatcctg aatacgaatc gcgttatttt agatatgcaa gcaacgaata aagcggaggc
     1741 gattgaagaa ttaactaatc ttctcaaaaa agatggtgcg atcgattgtc gggaaacctt
     1801 tattaaagat gtctggcagc gcgaagcgga aggctcgact ggttttgaaa atcatattgc
     1861 gatcccgcat ggtaaatctt ctgcggtaat taatactacg ctggcaattg gccgtacccg
     1921 ccaggatatt ccctgggaaa cgctggatgg cagtagcgtg cgctgtatta ttttatttgc
     1981 ggtacgtctt gaagatcaaa ataccaccca tatccgttta ctttcccagg tcgcgggcgc
     2041 gctggcggat gatgacatca tagaacaatt gttagttgag acgagtccgc aaaaaatcat
     2101 tgatctcttt agtcagtatg cacaatccga tgtcttctaa ataacaggga gtatgtcatg
     2161 aatattgtct gtgttgccgc ctgtacggcg ggtattgctc atacctatat tgctcgtgaa
     2221 aagttaatca aaggagcaaa agctctgggc cataccatta aagttgaaac ccagggaacc
     2281 attggtacag aaaatgaatt acaggcggca gatatctctg ctgctgacgt cgtgattctt
     2341 gctgtagatg tgaaaataaa aggagaagaa cgcttcacca ataaacgtat tgtgcgagta
     2401 aagacagaga tcgtcattaa atcgcccgtt caatttcttg aaaaggtcga aaaatcactg
     2461 ggaaacaaat gatccttata ctggagggat cttaatatga aagaaaataa aattccggtt
     2521 tcacaggaaa ttaaaaaaca tttattaacc ggcatctcgt ggatgatacc ccttattgtg
     2581 gccgccggga tatgtattgc gttgggacaa gttcttggcg gtacagatgt tggtgaaaaa
     2641 acaggaacaa tcccgtggat gcttaatcag attggcggct ggggaatggg gctgattgtg
     2701 ccgctgatta gcgcggcaat tgcctattct attgccgatc gtcccggatt tgccccgggc
     2761 ctgattgtcg gctttctctg cgggcaaatc cataccggat ttattggcgg tatgttgggt
     2821 ggtttcctgg tgggctacac cattttgcta cttaaacgct atatccgcct gccgcagtcg
     2881 atgcaaggac tcatgccaat catggtgttg ccggtattaa gcaccattat cggtgggctg
     2941 ttaatgatga cgctgattgg taaacccatc gcctggttac aagaagcact gattcatttg
     3001 ctcgagtcga tgcagggtgg atcgcgcttc ctgatggggg ccatacttgg cgcgatggca
     3061 accttcgatt tcggtggtcc ggtcaacaaa accatgtcgc tgttttccga tggcttgctg
     3121 gtcagtggcg tctacggacc agaagccgtg aaatttgtcg gctcaatcat ccctccgttt
     3181 ggcatcacac tttcgttctt actgacacgc cataaataca cccgtgcgga gcgcgaggcg
     3241 ctgaaagccg cgtttccgat ggggatctgc atgatcactg aaggagtcat tccgatcgcc
     3301 gcgcgtgacc tgctgcgagt agtgggatct tgtgtggtgg catcggcggt cgcgggcggc
     3361 ttaatcatgg tgtggggcgt ggagagtccg gttccgcatg gcggcatgtt tgttgtgcct
     3421 ctgttcaccc atcctctgct gttttgcctg gcgctgggta taggcacggt gatttgtggc
     3481 gtgatgcttt cgctctggaa gaaaccggta acggaacgcg acgaagagtt tgacgaactg
     3541 agcgatcaaa agctgaaaga tgaagagatc actttcaccc tcgaataacc ggaggcccta
     3601 tgtttgccga tatgaaaagt atggtgatga aagcctggca tgaacattac gccttgctgg
     3661 cgatcaactg tatgaatctg gaaagtgctc acgcagctat ccgcgtggcg gagaagaatc
     3721 gcgctccgat cattctcaat ctctatcagg ggcatctggc gcattttcct gcacccgtcg
     3781 cggcggcagt ggttaaaacg ctcgcggaat ctgcctcagt accggtcgct ctggcgcttg
     3841 atcatggcaa aaatccggac tgtattcgtc aggcgtttcg tgctggtttc agcggattaa
     3901 tgattgatgc ttctgctttt cccctggagg aaaacgtgcg ccagaccagg gcggtggtgg
     3961 aactcgccgc cagcgcgcag ctctgcgtgg aaggcgaact gggccatctg gcagatgcgc
     4021 cgcgttacga ccaggccgcc aatgcagacc tgatgacgca acccactgat gtggaaccgt
     4081 ttatcgcgca gacaggcatt gatctactgg ccgtctcggt aggcacggcg cacggcatgt
     4141 acgcgcctgg tgttgtacct gctattgatt ttcagcgtct ggcggagatt ttccactgct
     4201 cgagcgtgcc tctggcatta catggcggta gcggtacgcc gttcgatcag ctccagctct
     4261 gtacgactct gggggtagca aaaatcaacg tgggggcggc catttttgaa cgggggaaat
     4321 ccgcgctcct gcataccctc tgtcacgata tatccattga actggtggac gcgctgaaag
     4381 ctatggaact ggccttcgaa gaagcgatta caccgtattt acaggcgagc ggcgctatcg
     4441 gcaaagcctg attcattttc tgtcatggcc tttatgccag tgcaataacg caccggcagg
     4501 gcggactaca cgctgaaact ggagatttaa catgttagtt tcgatgaaag aattactgca
     4561 acctacccgc cagcacggct ttgccattgg cgcatttaat gtggcggata actgtttcct
     4621 tcgcgcagtc gtggatgaag cagaagcgac aaatactccg gcgattattg ccatccatcc
     4681 cagcgaacat gattttgtgg gcgatgcctt tttcgctcac gttcgcgaaa ttacccagcg
     4741 tagtccggtc ccgtttgtac tgcacctcga tcatggtgcc tctgtagagc atgttttgcg
     4801 tgcgattcgc tgcggattta cctcagtaat gatcgacggt tcgctgctgc cgtatgaaga
     4861 aaacgtggcg ctgaccgcag aagtggttcg cctcgctcac gcggttggcg tatcggttga
     4921 gggtgaactg ggaaccatcg gacaaacagg aacgtcggta gaaggtggtg tttcacaagt
     4981 gacctacaca gatccggcgc aggccgccga ttttattgct cgtacaggtg ccgacacact
     5041 ggctgtggcg attggtacag cgcatggcat ctatccaaaa gggatgcagc cgaagctgca
     5101 aatggacatt ttgcgcgaca ttgccggacg tattgatatt ccgctggtgc tacacggtgg
     5161 ttcggcaaac ccggacgcgg agattgctga atcagttacg ctcggcgtcg gcaaaatcaa
     5221 tatctccagc gatatgaaat acgcctattt ccagaaagtg cgtgaaattc tcgctaaaga
     5281 gagctggtgg gacccgaacg tcatctatcc ggatacgatt aacgcggcgc gggaagtcat
     5341 tcgccacaaa atgaaactgt tcggttcgct cggtaaagca tcactgtact aagccagaag
     5401 ggcggggtga cccgcctttt acgcaacaag gaacgagata tgcactatct ggggttggat
     5461 attggtggga ccaaaatcgc cgccgttgtc atggatgcgc atggctggga gattcgccgt
     5521 taccgctgcc cgacgcaaaa gtcgacatat caacaatttg tctcatgcgt tgtggcgctt
     5581 atcgagcaga ttaggcggga cgttcaacga ccgatgctga cagggatcgc cttacccggc
     5641 agtatctcgc cactcactgg cctgattaaa aacgcgaata ttcaggtgat taacggtcat
     5701 gcgttacagg ctgatttgca gcaattgctt gggcaaccgg tggtgatagc caatgatggt
     5761 aactgtttcg cgctatcaga agcttgcgac ggtgccgggc aagattatga cgtggtattt
     5821 ggtattacgc ttggtacggg ctgcggcggt ggcattgcca tcaagcaacg accgtttata
     5881 ggggcctggg gaaatgctgc cgaatgcggt catatcacgt tgccaggcta tacggagcag
     5941 gaagatggtc catcagtcag ttgctattgc ggcaaacaca actgcgtgga gtcgtttgtt
     6001 tccggcagcg gttttagtga acgctatcaa cagatgactg gtaacttgct cactcctgcg
     6061 gcgattgtca ccctggcaca acgtggtgat gcttgtgcca tgcagcaggt ggcacgtttt
     6121 cgccaacagc ttgcccgcac gctggcaacc atcgttaacg ttgttgaccc tggcgtgatt
     6181 gtgatcggcg gcgggctttc gaatgtggaa ctgcttatca ccgatctgaa cgcagaagtc
     6241 gctccactgg ttttcaccga tcaattcacc actcccattg taaaagcaca gcacggcgac
     6301 agtagcggaa tgcgtggcgc tgcctggctt gctatgcgca acggagaagc caatgaaacg
     6361 ttcacaaatt aattatgtta ttgataaagc acatgccatt gcgcagacct tccgcgtttg
     6421 tttgccagag ttcgcctatt ttacggtgga tgactggctg cagcgggaac gggataactg
     6481 gcaagaagtg gtcgacttac agctcggttg ggatatcacc gattttggtc gtggtgattt
     6541 taaccaaacg ggactaacac tgctaacgat gcgcaacggc gcgctggggt cggcggcata
     6601 tccaaaaccg tatgctgaga aaatgttgca gatccaacaa gatcagcaga ctccctggca
     6661 ttttcacacc cacaaaatgg aagatatcct caatcggggc ggtggagact tatgcatgca
     6721 gttggcctgg gccaacgaag ccaatctctg tgatgatcaa cgagtcatta ccgtgagtgt
     6781 tgatggtcag cggcgtacca tgaaacctgg tgaaacgctg gtgctaaagc cggggcaggg
     6841 catttgcctg ccgccgcgtc tgtatcatcg cttctgggcg gaaaaagcgt ttgttctcgg
     6901 ctgggaaatc tcgatggtaa atgatgacca acacgataat tattttcttg agccgggagg
     6961 acgttttccg gcgatagaag aagatgaacc ggtgaaatgg ttattgtgcg gtgagtatgg
     7021 aattttgcgc taataacggg gaacgctagc gctgatatcc gctagcgtcc ttatttttac
     7081 tgcaccggct ccgtcgacgt aacttcacca ctggaaacta cgcccgcatt catggtttga
     7141 gtcacttgct gctttgcggc attcacggca tgcaattgcc cgctaaccgt aggtttcagc
     7201 gggacattga cctgaatatc gccactggcg gtaagctgaa tattgccgtc gccagtcaac
     7261 ggtaattcag gccatcccca atgttgcaaa atattcaccg gcacaccgcg tccattcagg
     7321 ctgatatggg tctgacgttg tggcgtttgt gaaacactgg cggtggcttc cagaatgcct
     7381 ttttcagtaa atgcactcag ttcgctgata ttcaccgtgc tgctgttggc agttagcgcc
     7441 agcgacggac gacggacatc aacacgatta aacgttgcgg cggcggcatt cagattcgcc
     7501 gagccactcc agacgcccca tttatgatcg gtaaccagcg tcagattggc accgtaacca
     7561 tcgagcgcgg tgagctgcca cgggaagtca gggtcgatat caatgatcag attgcggctg
     7621 gcgctaaatc tcttcagttg caggctgttt aaccaaccgg gtgtcgtttc catccacaac
     7681 tgttgccagt ttttgggcaa ggtatattcc agtccggcaa ttgccgcatc atcgaggatc
     7741 agcgttttcc cgtcgcgttg ccagtttcct gacgttctga ccataccccc ttcccagcgg
     7801 ctggtgaact ggcgcagtgc tacgccctgc ggggaaaatt ccgcgtttat aatcggatca
     7861 aataaatgca gcgagccata aataaactcg ctggcgttca tcgacagttt accttcctgt
     7921 gtctgccagt catctttact gaaggtcatg ttgcgcaagc tgagatcgag gtcggccacc
     7981 gcccagtccg gaccttgcaa acgagcgtcg atgacttcca ggcgaccaat ctgcaacgac
     8041 gggacagagc gtaatggtgc aaagaagtcg gttagcgatt tttcgctttg tagacggata
     8101 tccgccatgc gcaggttttc cacttgccag ctgccgtcag cgttacgctg cgcgtttccg
     8161 gttaatgtcc cgcgggcgat gtcggcaccc aggttagtca gcgtaacgcg atcgttatca
     8221 atactgcctt caatcagtac attggtggca ggaacatcat tgagcgaaag cgatccggca
     8281 ctaaactgaa tctgtgcctt cgtacccagc actttaccgg cttctggtga ccacggaacc
     8341 acgccgccat ttacccgctg cgcgctcagt ttccattcgc tattcgggct attaaacgcc
     8401 atatcacgca gttgcagacg atcggctttg aacggtagcg gcgcggtctg gtcggtgaga
     8461 ttcagcgtcc cgttttccag caggatggta tcgacatggc gtggttcggt cagttgccga
     8521 ctgcttagcg caatgtcgac actttttgct accagggtcg cgggctggcc atcacggcca
     8581 aacgtgacgt tctccagcac gatatgagat ggcgcggaaa aacggtgatc catcgccccg
     8641 aaggccagat gatagtcgct attctcggaa acccatgcgc tgatatgttc tgctccccag
     8701 cgagtttgca gaagaaaata gaggccagcg atcaccacta acagagcgat gagaatgtag
     8761 agaagcagct tcccaataaa tttcatggtc ttccatcccg tgaaatgcac ataagggagt
     8821 tatgcacgat ttacgcgcaa tcctcaaggc gggaatggtg aaagaggtga gactgcggcg
     8881 gtaattatct taccgccgcc agtgaattac tgtttttctg gtgggaaaat cagattcagc
     8941 acgatggcag taataccgcc cgcggcaatc ccggaggaga gcaggttttt cagccattca
     9001 ggagcaaact gcaaaatcag cggctgctga gacacgccca gaccgaccgc cagcgacagc
     9061 gcgataatca gaatcgcccg gcggttcagc ggctcacgag aaacgatacg cacaccggag
     9121 gcggcgatgg tgccaaacat tacaagcgtt gcgccgccca gaaccggttc tggaatgtgt
     9181 tgtacaaaac cgctcactgc cgggaacaga cccagcacga tcaacatcag cgcgacgaca
     9241 aaaccgacat agcggctggc aacaccggtt aactggatca ccccgttgtt ctgcccgaag
     9301 caggagttcg ggaaggtatt aaataccgcc gagacaaacg agttcaggcc gtttgccagc
     9361 acgccgcctt tcagacgttt catgtacagc ggaccggaca ctggctgttc ggaaacgtca
     9421 gaggtcgccg tgatatcgcc aatggtttcc agcgaagtga tcataaagac cagcatcagc
     9481 ggcagcagca gactccattc aatgccaaga ccgtaataga gcggcgttgg caccataatc
     9541 agttcttgtg tcatcggttc gttgctttct ggcaacatgc ccataaacca cgccagcgca
     9601 tatccagccg ccatcgcaat caccagtgag gccacgcgta agtaagggtt acgttgacgg
     9661 ttaagcagga taattaaggc taagaccacg cctgccagca gcagattttt cggtgcgccg
     9721 aaggtgttat cgctcatggc tgcgtaaccg ccgccaatgg aggtcagccc aacctgaatt
     9781 agcgacaggc cgataatcat caccacaacg ccagaaacca gcggcgtaat aatgcggcgc
     9841 gccagatgca gaacgcggga gatcaccatc tcggtacaac ttgccagcat caacgtgccg
     9901 aacaaagctg ccatcatggt aggaacatca gcaccaccgg tcttcagcgc tgtgccgccc
     9961 ataatcagcg gagcaacaaa gttgaagctg gtgccctgaa tagacaacaa tccggagcca
    10021 accggacccc aggccttaat ttgaataatc gatgccacac cggaggcaaa cagcgacata
    10081 ctaataatgt gttgcgtgtc ttgtgccggt aaacccagcg cctggcagat taatagcgct
    10141 ggcgtgatca ccgcaacgaa catcgccagc aaatgctgac aggcggcaaa cagggtttga
    10201 ggaagcggcg gacgatcttc aagacggtaa atcagttcgc tgttttgagt ctgcgcaacc
    10261 ggttgcgcat tttctgactc gagggtggaa acagacatcg aaaacaatcc cgtggtagca
    10321 aagcgggcat tttagctgac cgaaagagaa aagcaaacgt ttgccacatc ctcatctaaa
    10381 tatttaaatg agataaatgt acttttttat ataactttta gttgttttct gcttaaaatc
    10441 catgccattt ttctattttc agctaagaat tcgaaccagg aaagcatgac ccgatactcg
    10501 tcatatttcg ccttactgca aatgcgcgat gaagtatgac gagtatgaaa gagtgatgcg
    10561 gacacaaagg agtaactatg tttcatctcg atactttagc aacgcttgtt gccgcaacgc
    10621 tgacgttgct gctcgggcgc aagttggtcc attccgtctc ctttttgaag aaatacacca
    10681 taccggaacc tgttgcgggt ggtttattag tggcgctggc gctgctgata ctgaaaaaaa
    10741 gcatgggctg ggaagtcaac tttgatatgt ccctgcgcga tccgttgatg ctggctttct
    10801 tcgccaccat tggcctgaac gccaaccttg ccagtctgcg agctggtggg cgtgtggtgg
    10861 gggttttcct gattgtggtt gttggcctgt tggtgatgca aaatgccatt ggcattggta
    10921 tggctagcct gttagggctt gatccgctga tggggctgtt ggccggttct attacgcttt
    10981 cgggcggtca cggtacaggt gctgcatgga gtaaattgtt cattgaacgt tatggcttca
    11041 ccaacgcgac agaagtggcg atggcctgtg caacgttcgg tttggtgctg ggcggcttga
    11101 ttggcggtcc ggtggcgcgc tatctggtga aacactccac cacaccgaac ggtattccgg
    11161 atgatcagga agttccgact gcgttcgaaa agccggatgt gggccgcatg atcacctcgc
    11221 tggtgctgat tgaaactatc gcgctgattg ctatctgcct gacggtgggg aaaattgttg
    11281 cgcaactttt ggctggtact gcttttgaac taccgacctt cgtctgtgta ctatttgttg
    11341 gcgtgattct gagcaacggt ctgtcaatga tgggctttta ccgcgtcttt gagcgagcgg
    11401 tatccgtgct gggtaacgta agcctgtcgt tgttcctggc gatggcgctg atggggctga
    11461 aactgtggga gctggcttcg ctggcgttgc cgatgctggc gattctggtg gtacagacca
    11521 tcttcatggc gttgtatgcc atcttcgtta cctggcgcat gatgggcaaa aactacgatg
    11581 cggcagtgct ggctgcgggt cactgtggct ttggccttgg tgcgacacca acggcaatcg
    11641 ctaacatgca ggcgatcact gaacgctttg gcccttcgca catggcgttc ctggtcgtgc
    11701 cgatggtcgg tgcgttcttt atcgatatcg tcaatgcgct ggtgattaag ctgtatttga
    11761 tgttgccgat ttttgccggt taaccgatga agcggcggtc ttctcaccgc cgctttttac
    11821 gcattcgagt aacgttctgt ttctggcatc cagcgttcta tcagggcttt tgcctgttgt
    11881 gggtaacgtt cgtgaatatg tcgcgccagg cgctgaactt ccgggatcat cgcctgatcg
    11941 cgcagtaaat ccgccacttt aaattcagca ttacccgtct gacgtgtacc taacaattcg
    12001 ccagggccgc gaatttccag atctttctgt gcaatcacaa aaccatcgtt gctgtcgcgt
    12061 agtacctgca ggcggatttg cgcggttttg gagagcggcg ttttgtagag cagcacgcag
    12121 tgagaagcca ccgcgccacg acccacgcgg ccacgtaact ggtgtaactg cgccagaccc
    12181 agacgctccg ggttttcgat aatcatcaga ctggcattgg gtacatcaac accgacttca
    12241 atgacagtag tggcaaccag caggtgtagc tcgccttgtt taaacgatgt catcaccgcc
    12301 tgtttctcgg cgggtttcat ccgtccgtgt accaggccaa cattcaactc tggtagcgcc
    12361 agtttcaact cttcccaggt cgcttccgcc gcctgcgctt ccagtaattc cgactcttca
    12421 atcaacgtac aaacccagta cgcctgacgc ccttcagtca tacaggcgtg gcgcacgcgg
    12481 tcaatgatat cgatacggcg ggtatcagga atagcgaccg tagtcactgg cgtgcggcct
    12541 ggtggcagct catctatcac cgaggtatcg agatcggcat acgcagtcat cgccagcgtg
    12601 cgggggatgg gcgtggcggt catgatcaac tgatgcggat ggaagccctg ctgctgacct
    12661 ttctcccata acgccagacg ctgatgcacg ccaaaacgat gctgttcgtc gataatcacc
    12721 agcgccaggc cgttaaactg cacctgttcc tggaaaatgg cgtgcgtacc aacaatcatc
    12781 tgcacctgac cgctggcgat ggcttcttgc tgagccagtc gtgctttacc tttctgtttt
    12841 ccggcgagcc agcctacttc gataccgagc ggtgcaaacc agttacggaa gttattggcg
    12901 tgctgctcgg cgagtaattc ggttggtgcc atcagggcca cctgtttgcc gtgggcaacc
    12961 gcacgcaacg cggcgagggc ggcgaccagc gttttaccgg aacctacatc gccctgcacc
    13021 agacgcatca tcggcacatc tagcgccata tcgcgctcga tctccgccac tacgcgtgcc
    13081 tgtgcgcccg ttggcttgaa cggtaaggcg gcgaggagtt tatttttcag cgcgtcattg
    13141 gcgctcagcg gctgagcatg aaaacgttgt gcaccggcac gtaacgccaa catgctgagg
    13201 ttgtgcgcca gcaactcttc cagaatcagc cggcgttgtg ccggatgctg cccggtttcg
    13261 agatcgctaa gctgtagcgt cggcggcggg cggtgcaaag tgcgcaacgc ttccggtagc
    13321 gtcatcattc cttgtgacag ttccggcggc aggagttctt caatggcgca tgtgtcgagc
    13381 agatccagcg cctggtcggt taatttacgc agcgtagcct gctttacgcc ttccgtagtt
    13441 ggataaaccg gtgtgagcgt ttcctgtaac tctggcgtgc tgagatcgcc ctgtacgcgg
    13501 tattccgggt ggatcatctc cgcaccatat ttgccgcgct ttgcttcgcc ataggccagc
    13561 acgcggcggc ctgtcgccag gctatttttc attgccgcat tgaagttgaa aaagcgcata
    13621 gtgagaatgc cggaaccgtc gctgatctgg caggtcatca tccgccgacc gccgaaggag
    13681 atattgcagt tcagcacttc gccttccacc gtggcataaa cacccggcag tagttcaccg
    13741 atggggtaga gatgtgtgcg atcttcgtag cgcagaggaa ggtgtaagag caaatcctgt
    13801 acggtatgca ggttgatttt cgccagcttg ttactaagcg ctgcgccaac gcccgttagg
    13861 gaactgagcg ggacagcatc taacaggcga cctttcatcg cgcttaccct gcggcctgca
    13921 tggtggacca ccagtcagca tcggcttcaa tctcgccttg ctgattaacg tgggggtagg
    13981 gcagaccttt gcgttttgcg actttcgcca gcaccggata gccgccttca aacagcaggc
    14041 gttgttgctc ggcttccggc aacatgctgt tttcacgcag gtacatgccc gcattttgcc
    14101 gctggcgctg ggcttcgtaa agaatgaggg ctgaggcaac ggaaacattc agcgactgca
    14161 ccatgccgat catcggaatg atgatatcct gatccgccag ggccaacgct tcctgcgtga
    14221 tgcccgtttt ctcctgtccc atcaaaatac aggtcgggcg agtgtaatca atttcgcgga
    14281 aatcaacagc gttatcagaa agatgggttg ccagaatctg catgccctgg cctttcagat
    14341 gagcgacggc atcgccaatg gtgcggtgcg ttttcacctg tacccagctg ttactacccg
    14401 cagctgccga agccatggtg cgcatccggc tgccaggcca gacagcatga acttcatgta
    14461 cgccaacggc atctgcggta cgaataatcg cagaaacgtt atgaggtttg tggacctgct
    14521 ccatgcagac ggtcagatca ggctgccgcc tggcgagcat ttcgcagatg cgtgcataac
    14581 gtgttgggtt cataaaacat taatttcggt ttcgggtgac tttaatcacg tctggcatca
    14641 cgcggatttt gcgcatgata ttcgccagat gcacacggtc acgggcggtc agacgaataa
    14701 aggcgctata gacgcgacca tctttctctt ccgtattcaa actttgaata ttcgaggtcg
    14761 tggtgttaat tgccgccgtc aggtttgcca gcgcgccctg atgattgaac atctccacct
    14821 tgatttcggt gatgaattcc tgcgccgtct ctttatccca ttcaacagcc ataaacttct
    14881 ctggctcttt ctggtagcca cggatgttac ggcaggattc atggtggatc accagacctt
    14941 taccggggct gacgtgggcg ataatcgggt cgccaggaat tgggcggcag catttcgcaa
    15001 aggtgatcag cacgccatcg gcacctttaa tgggcagatg tccgtgactt tgggttgccg
    15061 gtggaatgga ggcgtcccca tgttgcagat ttttcgcgac caccacgctc attgcgttac
    15121 caaggccgat ttctgccagc agatcgtcaa gcgttgccag cttcatgcgg tccagctcgc
    15181 gctgaatatt ttcctgcggg atttcattga gcttacggct accacccaac gcatggttga
    15241 gcagacgacg gcccaggctt acagaatcat cacgcttgag gtttttcagc aactgacgaa
    15301 ttttggcgcg cgctttcgag ctgacgacaa agttcagcca cgcggcattc gggcgagcac
    15361 ccggtgcggt aatgatttca acggtttgac cgctagtaag cggctgcgac agcgggtaag
    15421 gctggcggtc gacgcgtgcg cccacgcagg catggccgat atcggtatgc accgcataag
    15481 cgaagtcgac gggcgttgca ccggcaggca gttcgacaat gcgcccttct ggtgtgaaaa
    15541 cgtaaatctc atccgggaag agatcggatt taacgctctc gataaattca aacgagctac
    15601 cggcactctg ttgcagctcc agcaggcttt gcatccagcg ctgggcgcgt atttgcgcgg
    15661 tagtgctggt ttcgccgtgc tctttataag cccagtgcgc ggcaacaccc atctccgcca
    15721 tctgatccat atcttcggta cggatctgga cctcaaccgg cacgccgtgc gggccaatca
    15781 tcgaggtgtg caaagactga tagccgttcg cttttggaat ggcgatatag tctttcacgc
    15841 ggcccggacg cggcttgtac aggctgtgca tctggcccag cacgcgataa caggtgtcag
    15901 aatcattgac gatcacgcgg aaagcgtaga tgtccatgat tgagtgaaaa cgctgctctt
    15961 tgagcaccat tttgcagtaa atcgaataaa gatgcttttc gcgaccactg acgcggcacg
    16021 gtattcccgc ttcctgcaaa cgcccttcga tttcagaaag aatcttctgg atcatctctt
    16081 tacggttgcc gcgcgcggct ttcaccactt ctttgattac gcgataacgg ttgggataca
    16141 gcgcctcaaa acccagctcc tcgagttcgg ttttaatgtg gtggatacct aaacggtgcg
    16201 ccagcgggct gtaaatttcg agagtttcac gggcgatgcg gcgacgtttg tccgggcgaa
    16261 gtgagcccag cgtgcgcatg ttgtgggtac ggtcggcaag tttgatgaga atgacgcgga
    16321 tatcctgcac catcgccata atcatcttgc gaaagttttc ggcctgcgcc tctttcttat
    16381 cgcggaactt gagtttatca agtttcgaca ccccctctac cagctcggcg acgcttttac
    16441 caaaaagctg ttccatgtcc tggtaggtgg cgggtgtatc ttcaatcaca tcatgcagca
    16501 gcgccgccat cagcgtttca tagtcgagtt tcatctcggc cagaatgcag gcaaccgcca
    16561 ccgggtgcgt gatatagggt tcaccgcttg aacgtgtttg cccctcgtga gcatcacgtg
    16621 caacgagata cgcctgccgc agacgcttga tttggtcttc cggcaggtag ttttgaatca
    16681 gctgattcag gctttcaaac agatacaagg gcgacccgct ttgtgattaa cgacgacctt
    16741 cagcaatagc ggtaacggct tgtaattcag cggcttcctg ctcttgctgt tcctggcgtt
    16801 cgcgaacgtc gaggatctgg ttgttgatca gaccttcttc gatttcgcgc agcgcgatta
    16861 cagtggtttt atcgttttct tccggtacca gcggatcctt tccgcctacc tgcatctgac
    16921 gagcgcgacg cgcggcgacc agtaccaggt caaaacggtt accaattttc tctacagcgt
    16981 cctgaacagt tacgcgtgcc atacttaaaa agctccacag gtgaagaaat gactgggcat
    17041 gatactgaaa tcaggttcag tctgccaaca atttgctgat taaagcgtca tgacgctgct
    17101 tttggcggct catgcgcaga cgttcggcgc gaataatggt cttcaaatcg gtcaacgcgg
    17161 tatcgaagtc atcattcaca atcaggtaat catattcggc gtaatggctc atttctgcaa
    17221 cagcttgcgc catacgcttt gcaatgacct cttcgctgtc ctgaccgcga ccgcgtagac
    17281 ggcggtccag ttcgatttta gacggcggta aaataaagat actccgcgcg tgcggcatct
    17341 tctggcgaat ttgctgcgca ccctgccagt cgatatcgag aaaaacatcg acaccggtcg
    17401 ccagtacttg ctcaatggcc tcacgcgaag tgccatagta attaccaaaa acttctgcgt
    17461 gttcgaggaa cgcatctcta ctaatcattt ctttaaattc atcatgatta acaaagaaat
    17521 aatgttcacc gtggacttca ccaggacgcg gttggcgtgt ggtgtgtgaa acagaaacct
    17581 gggtgtcata caacggttgg gtttttaata aagcctgaat caggctggat ttacccgcgc
    17641 cactgggggc agaaacaata taaagcgtgc cttgagccat gagtatcttt cgtatgtgat
    17701 tagcgaaata aagcctacat acgagcttat tatacacggc gtggccatgt gacgtagccc
    17761 ttgctcacac tttttgcgca aagttgttgc attttgcgta gctgcttccg catttgcctg
    17821 tgattcactg caataaagac gattctcagg aagcgtcctc gcagactccg tttttgccac
    17881 tcggctttgc cttcaccacg ttgtatatct gcctgactat ctacagcgga ggtgatgatg
    17941 aaagtatgga tggcgatatt aataagtatc ttgtgctggc aatcatcggc gtgggcggtc
    18001 tgtccggcct ggtcgccagc cagagcacag gaagaaattt cccgcctgca acagcaaata
    18061 aaacagtggg acgatgacta ctggaaagaa gggaaaagtg aggtggaaga cggtgtttac
    18121 gatcagttaa gtgcccgtct tacgcagtgg caacgctgtt ttgggaacga gactcgcgat
    18181 gtgatgatgc cgcctttaaa cggtgcggtt atgcatccgg ttgcccatac gggtgtacgc
    18241 aaaatggcgg ataaaaacgc attaagtctg tggatgcgcg aacgtagcga tctttgggtg
    18301 cagccaaaag ttgatggcgt tgcggtaacc ctggtttatc gggacgggaa actgaacaaa
    18361 gcaatcagtc gcggtaatgg cctgaaaggc gaggactgga cgcagaaagt tcgcttaatt
    18421 tccgctgtgc cgcaaaccgt tagcgggcct ttagccaaca gcacgcttca gggggaaata
    18481 tttctccagc gtaaggggca tatccaacaa caaatggggg gaataaatgc ccgcgcaaaa
    18541 gttgctggcc tgatgatgcg ccaggggaat agcgatacgc taaactcact ggctgttttt
    18601 gtctgggcat ggccggatgg accgcattta atgaccgatc gtttaaagga tctggctacc
    18661 gcaggtttta ctttaacgca gacgtatacc cgcgcggtta aaaatgctga tgaggttgcg
    18721 cacgtacgca atgagtgggg gaaagcaaaa ttacccttcg tcaccgatgg cgtggttgtg
    18781 cgagcggcga aagaacccga atcccgtcat tggctaccgg gccaggcgga gtggctggtg
    18841 gcctggaaat atcaacctgt agctcaggtt gccgaagtga aggcgattca atttgcggtg
    18901 ggtaagagcg gtaaaatatc ggtggtggcg tcacttgtac ctgtcatgct ggatgataaa
    18961 aaagtccagc gggtgaatat tggttccgtc aggcgctggc aggagtggga tattgcgcct
    19021 ggtgatcaga tcctcgtcag ccttgccggt caggggattc ctcgcattga tgatgtggtc
    19081 tggcgcggag cagaacgtac aaaaccgaca ccaccagaaa accgctttaa ctcgttgacc
    19141 tgctactttg cttctgatgt ttgtcaggaa cagttcattt cacgcttagt ctggctgggg
    19201 tcaaaacagg ttctggggct tgatggcatt ggtgaggccg gctggcgcgc cctgcatcag
    19261 actcatcgct ttgagcatat cttttcctgg cttttattaa cgccagagca attacagaac
    19321 acgccgggga tcgcgaaaag taaaagtgcg cagctatggc atcagtttaa tctggctcgt
    19381 cagcagcctt ttactcgctg ggtgatggca atgggaatac cgctaacccg ggcggcgctt
    19441 aatgccagtg atgaacggtc ctggtcgcaa cttttattta gcacggaaca gttctggcag
    19501 cagctgccgg ggactggatc ggggcgcgcc agacaggtta ttgaatggaa ggaaaatgcg
    19561 caaatcaaga agctgggcag ttggctggct gcccagcaga tcacaggttt tgaaccttag
    19621 tgaccttcgt ggctgtagta aaagaccggt aatcccagct taagacgtag cgccagtaaa
    19681 cgggcaaaaa agccgaatac caaggtggag ataatcacca catcatggtt agaaacatag
    19741 tgttgcagcg cgatgtacag cacggcggag gcaaatgaga caccggcata caactctttc
    19801 tggaatacca gcgggatgcg tttacagaac atatcgcgca aaacgccgcc aaacacgcca
    19861 gtcgtcacag ccgcgacaac ggcaatgatc gggccgtgtc ccatatccag cgcgacctgt
    19921 gcgccgatga tagaaaagac caccagtccg agtgcatcga gcaccagaaa gactttacgc
    19981 aggtaaggca tcacgggagc aaagatagtg gttaacaccg cagcggtagc gacgataatc
    20041 acatattccg ggtgtttgac ccaaccgagc ggataatggc ccagcaggat atcgcgcaca
    20101 gaccctccgc caattgcagt ggcagtcgca ataataatta cgccaaatgt atccatgcgc
    20161 cgccgaccgg ctgccagcgc ccccgtcatc gcttcggcgg taatgccgac caaatacaaa
    20221 atgtgtaaca gcatgggctc cccctgattt actgcacgga agagtagcga tttgcgcgca
    20281 ctgtcacgat tgagattttt taaggtgaga agggattgat aatttgtagt tatgtgaaat
    20341 tgctgttttc tgtagtgtga atgcattttt ctaattttgt gataagtaaa atgcattaga
    20401 aaaagtatgg agcaattaaa tgtgaggacg aaattatatg cgtagagccg ctgtaattaa
    20461 caacggccta cggagatatg ttgtttactc gatattctga atctgctcgc gcatctgctc
    20521 aatcaacact ttcagctcga tggcagagtt tgtcacttcg gcattgatag acttcgacgc
    20581 aagagtgttc gactcgcggt tgaactcctg catcataaag tccagacgac gaccaacggc
    20641 ttcttttttc ttcagaatgt tgtaggtctc tttgacatgc gcttcgaggc ggtccagttc
    20701 ttcggcaacg tcaattcgtt gtgccagcag aaccaactcc tgctccagac ggttgttttc
    20761 cagttgcacc tgagcatctt ccagcttcgc taccagacgc tcacgctgcc attgcaggat
    20821 ttccggcata tgggcgcgga ctttgaccac ttcggcggtg acgccttcca gacgctgctc
    20881 aatcaacact ttcagcgcct gaccttcggt ttcgcgcgcg atgataaagt catccagggt
    20941 gccatccaac gccgcgagaa tttcagctgc aatcgcgtcg agatcttgct cctgggctgc
    21001 catcacgccc ggccagcgta gaatatcaac cgggttgatt tctccttcgt cgctctgcat
    21061 ttttacccag ttcgcggcag ttaccagctg tttagccagt ttttcgttga ggatcagctc
    21121 accttgcgcg ctaacatctg gctcatagcg cagagtacat tccactttac cgcgcgtcag
    21181 gcgagaacga atacgctcgc gaacgacagg ttcaaggcta cggaactgct ccggcagacg
    21241 aaagtaagtt tccagataac gctggtttac cgagcgcatt tcccaggttg cgctccccca
    21301 ttcacccttg atttcacgcc gggcgtaggc ggtcatactg cggatcatag acgttcctgt
    21361 ttataaaagg agaggtggaa ggattatagc catcgatgcc ttgtcaggat aggaataacc
    21421 gccggaagtc cgtataatgc gcagccacat ttgtttcaag ccggagattt caatatgcgt
    21481 ccagcaggcc gtagcaataa tcaggtgcgt cccgttaccc tgactcgtaa ctatacaaaa
    21541 catgcagaag gctcggtgct ggtcgaattt ggcgatacca aagtgttgtg taccgcctct
    21601 attgaagaag gcgtgccgcg cttcctgaaa ggtcagggcc agggctggat caccgcagag
    21661 tacggtatgc tgccacgttc tacccatacc cgtaacgcgc gtgaagcggc gaaaggtaag
    21721 cagggtggac gcacaatgga aatccagcgt ctgatcgccc gtgctcttcg cgcggcagta
    21781 gatttgaaag cactgggtga gttcaccatt acgctggact gcgacgtgct tcaggctgat
    21841 ggtggcacgc gtaccgcttc gattacgggt gcctgcgtgg cgctggcaga tgcgctacag
    21901 aagctggtgg aaaacggcaa gctgaaaact aatccaatga aagggatggt agccgcggtc
    21961 tctgtcggga ttgtgaacgg cgaagcggtt tgcgatctgg aatacgttga agactctgcc
    22021 gcagagaccg acatgaacgt agtgatgacc gaagacgggc gtatcattga agtgcagggg
    22081 acggcagaag gcgaaccgtt cacccacgaa gagttactca ccttgttggc gctggcccga
    22141 gggggaatcg aatccattgt agcgacgcag aaggcggcgc tggcaaactg atttttaagg
    22201 cgactgatga gtcgcctttt ttttgtctgt agaaaagtaa gatgaggagc gaaggcatga
    22261 aaccatatca gcgccagttt attgaatttg cgcttagcaa gcaggtgtta aagtttggcg
    22321 agttcacgct gaaatccggg cgcaaaagcc cctatttctt caacgccggg ctgtttaata
    22381 ccgggcgcga tctggcactg ttaggccgtt tttacgctga agcattggtg gattccggca
    22441 ttgagttcga tctgctgttt ggccctgctt acaaagggat cccgattgcc accactaccg
    22501 ccgtggcgct ggcggagcat catgaccttg acctgccgta ctgctttaac cgcaaagagg
    22561 caaaagacca cggtgaaggc ggtaatctgg ttggtagcgc gttacaagga cgcgtaatgc
    22621 tggtagatga tgtgatcacc gccggaacgg cgattcgcga gtcgatggaa attattcagg
    22681 ccaatggcgc gacgcttgca ggcgtgttga tttcgcttga tcgtcaggaa cgcgggcgcg
    22741 gcgaaatttc ggcgattcag gaagttgagc gtgattacaa ctgcaaggtg atctctatca
    22801 tcaccctgaa agacctgatt gcttacctgg aagagaagcc agaaatggcg gaacatctgg
    22861 cggcggttaa ggcctatcgc gaagagtttg gcgtttaacg aaactcgccg gatgaaaagt
    22921 catccggcgt catattactg caactgtgcg gcaattagcg gccagcgggc gtcaaaatca
    22981 tccgtcgggc ggtatttaaa ctcgctgcgg acaaatcgtg acagcatacc ttcacaaaat
    23041 gccagcaact ggcttgccag cagggtttca tcggtggtgt aaccttcacc ctcacgcatt
    23101 ctcttttcac gcaatacctg acgcaactgc gcttcaatac gctcgaacag ctggttgatg
    23161 cgcccttgca gacgatcctg ttcgaacatt agcgcatgac cagtgaggat gcgagtcagg
    23221 ccaggattac gctcaccaaa accgaggagc aacaacacga tcagacgcag gcgcgctgtg
    23281 gtgtctttct catctttcag aatcaggttg atacgagtaa tcaggctatc ttcgataaac
    23341 tcaatcaggc tatcgaacat gcgggtctta ctggggaagt ggcgatacag tgccgcttcg
    23401 gaaacgccga cagaggcggc cagttttgcc gtcgtgatac gttggcttcc atcgctggat
    23461 tccagcatca gcgccagaga ctgaagtatt tcctcgcgac ggttcctttt cgcagtttgt
    23521 ttttctgcca tgttacaaaa tacccctgaa aataagcact tgccaggcgg cacccacgct
    23581 atgaccgcaa acgaaatgtt tgcggctatg ttatgacgtt attcggatgc gtatgtgtta
    23641 ctgacgacca gagtgaccaa agccgccttc accgcggtcg gtggcgtcga aatcttccac
    23701 cagattaaat tcagcctgta ctaccggaac aaaaatcatc tgggcgatgc gttcgccagg
    23761 ttgaatggtg aagctgtcct gaccacggtt ccacacggaa atcatcaact ggccctgata
    23821 gtcagaatcg atcaatccta ccaggttacc aagcacgata ccgtgcttat gtcccaatcc
    23881 ggagcgcggc agcatcattg ccgccagtga aggatcggca atatgaatcg ccagcccggt
    23941 cggaaccagc gtagtgtcac ccggagccag ttctacggcg tcgtcgagac aggcacgcag
    24001 gtcaagtccg gcagagccag aggtggcata agtcgggagc ggaaattcct tcccaacgcg
    24061 cgggtccaga atcttaacgt cgattttttt catcataacg ggtcacgatc tcgtcgagta
    24121 ataattggcc aaggagcgct ttgcgctcaa gcggtaagac tttatctccg tcctgccaga
    24181 aaaggtgtaa tgcgttgttg tcgctgttaa atccttgagt tggctgggaa acatcgttcg
    24241 cgcagatcag atcaaggttt ttacggatac gtttttgccg ggcgtattct tccacattat
    24301 ttgtttcggc ggcaaatcca acgacgtagg gtcgatggtc ttttagtgcg gcaacgcctg
    24361 cgacgatatc ggggttttta accattttta ttgttaattc atcaccctgc gtggcctgct
    24421 ttttgatttt ttctggggcc acggtagctg cgcgataatc cgccacggcg gcgcaaccga
    24481 taaaaatatt ttgctgctgt acagaagcat tcacggcggc ttccatttcc agcgcggtca
    24541 tcacatcaac acggttaaca aacggtggcg tcggtagtga aaccggacct gataccagcg
    24601 tgacgttcgc gccacgacgg gctgcggcgg cggcgatagc aaaacccatc ttgccggagc
    24661 tgtgattaga gatataacgc acgggatcga gcggttcacg cgtcgggccg gcggtaatca
    24721 taatgttcag atgtttcagg tcgttgacgg gcgaaaaatg cgccaccgcc atatccacaa
    24781 tggttaacgg atcgagcatt cgcccagggc cgatatcacc acaagcctga ctgccactgt
    24841 ctggccccca gatgagcaaa ccacgggaag caagtacctc taaattatgc tgcgtggcag
    24901 cggcacggta catctgctgg ttcatggcgg ggagcacggc tacaggcgca ggtgtagcca
    24961 gacaaatcgt cgataccagg tcattcgcca ttccggcagc aacacgggca attaaatctg
    25021 ccgtggcagg ggcgagaatc actaaatcag cccatttacc cagctcaata tggcccatag
    25081 cggcttcggc tgccgggtcc agcagactgt cggaaacggg gtaaccagaa accgcctgca
    25141 agctaagtgg ggtgataaag gcttttgccg cttcggtcat ggctacgcgg acatcggccc
    25201 cgcgatcgcg caaacgacgc accagttcag gggttttata ggcagcaata ccgccgctaa
    25261 cgccgagaac gattttttta ccggccaggc tcatgatgat ttttcctgtt gggtgacacc
    25321 agaagttggc gattttatca caatactttt gttgtcgtgc cttcacctga gattcacttt
    25381 gcgaggcgct ttccaggatt gaaaactggc cgtcgattta aaggaacggc tatgacagga
    25441 tgcgagcacc acaaaggagg tgaaggtgaa aaacaatgca cagctgttga tgccgcgcga
    25501 aaaaatgctg aagtttggta ttagcgcctt aacggatgtc gagctgctgg cgctatttct
    25561 gcgtaccgga acgcgcggta aagatgtatt aaccctggca aaagagatgc tggagaattt
    25621 cggctctctt tatggcttgt taacctctga atatgagcaa tttagtggcg ttcatggaat
    25681 tggcgtggcg aaatttgccc agttaaaggg gattgctgaa ctggcgcggc gttactacaa
    25741 cgtacggata cgtgaagaaa gccctttact cagcccggag atgacgcggg aatttttaca
    25801 aagccagctc acgggcgagg agcgggagat ctttatggtg atctttctcg actcccaaca
    25861 cagggttata acgcatagcc gtcttttttc cggcacgcta aaccatgttg aagtccatcc
    25921 tcgggaaatt atccgcgaag cgataaaaat aaacgcctcg gcgctgatcc ttgcgcataa
    25981 tcacccttcg ggttgtgctg aacccagtaa agcggataaa ctcattactg aacggataat
    26041 aaagagttgt cagttcatgg atttacgcgt gctcgaccat atcgtgattg ggcgtggaga
    26101 gtatgtttct tttgccgaac gcggctggat ttaacccgct atgcgcgatc cttcgggatc
    26161 tttgtctgtt cgggacttga gcacatcgct gagtcagcgt atactacgcc acctttgaga
    26221 atctcgggtt tggcatttgg gcctggcaat cgagagttca catagaactg cgatgaccgg
    26281 gctgtaaagc ctgacgaggc gccaataccc catacgaagc tcgagctaat ttgatttttg
    26341 gagaatagac atgtcccgag tctgccaagt tactggcaag cgtccggtga ccggtaacaa
    26401 ccgttcccac gcactgaacg cgactaaacg ccgtttcctg ccgaacctgc actctcaccg
    26461 tttctgggtt gagagcgaga agcgttttgt caccctgcgc gtatctgcta aaggtatgcg
    26521 tgtaatcgat aaaaaaggca tcgatacagt tctggctgaa ctgcgtgccc gtggcgaaaa
    26581 gtactaagta cttagaggaa ataaatcatg gctaaaggta ttcgtgagaa aatcaagctg
    26641 gtttcttctg ctggtactgg tcacttctat accactacga agaacaaacg tactaagccg
    26701 gaaaaactgg aactgaaaaa attcgatcca gttgttcgcc agcacgtgat ctacaaagaa
    26761 gcgaaaatca aataattttt gctttgatgt aacgaaaaac ctcgccccgg cggggttttt
    26821 tgttatctgc ctgcccccat attgactgca tctgttcatt cctggagatg ctatgcctga
    26881 attacccgaa gttgaaacca gccgccgcgg catagaaccg catctcgttg gtgcaaccat
    26941 tcttcatgcg gtggtgcgca acggacgctt gcgctggccg gtttcagaag agatctaccg
    27001 tttaagcgac caaccagtgc ttagcgtgca gcggcgggct aaatatctgc tgctggagct
    27061 gcctgagggc tggattatca ttcatttggg gatgtctggc agcctgcgca tccttccaga
    27121 agaacttccc cctgaaaagc atgaccatgt tgatttggtg atgagcaacg gtaaagtgct
    27181 gcgctacacc gatccgcgcc gctttggtgc ctggctgtgg accaaagagc tggaagggca
    27241 caacgtgctg gcccatcttg gaccggagcc gcttagcgac gatttcaacg gtgagtacct
    27301 gcatcagaag tgcgagaaga aaaaaacggc gattaaacca tggctgatgg ataacaagct
    27361 ggtggtaggc gtagggaata tctatgccag cgaatctctg tttgctgcgg ggatccatcc
    27421 ggatcggctg gcgtcatcac tgtcgctggc agagtgtgaa ttgttagctc gggtgattaa
    27481 agcggtgttg ctgcgttcga ttgagcaggg tggtacaact ctgaaagatt ttctgcaaag
    27541 tgatggtaaa ccgggctatt tcgcgcagga attgcaggtt tacgggcgaa aaggcgagcc
    27601 gtgtcgggtg tgcggtacgc cgattgtggc gactaaacat gcgcagcggg caacgtttta
    27661 ttgtcggcag tgccagaagt aattgatgcg agccggatgg cataccatcc ggcataaacg
    27721 ctacgctaac ttcgccatca gcgcctgatg gacattctcc ggcaggaaat gggtgacatc
    27781 gccctgatgg cgcgccacct ctttcaccaa cgatgaagag ataaacgacc actctttcga
    27841 cggcataaga aacacacttt ccagttccgg cattaagtgg cgattcatat gcgccagctg
    27901 catttcatat tcaaaatccg ccaccgcacg caggccacga atcagcaccg tagcgtgttg
    27961 attacgggcg aagttcgcca tcaaatcact aaacccgacc acttccacgt tccccagatg
    28021 cgcggttgcc tgctgtgcca gctccacacg ctcttccagg gtaaacatcg gttttttact
    28081 ggggctggcg gcaatcgcca gaataacgtg atcgaacatt tgcgtggcgc gcgtcacgat
    28141 atcgatatga ccattggtaa tgggatcgaa agtacccgga taaatcgccc gtttttgcat
    28201 aacaacctca atgcgttttc ggtggcaggt aaggttccag cagttgaagc agacgctgta
    28261 gtgcgccctg gttttgatac agcacttcaa cggcatgacg gccatagaaa ctacggtaat
    28321 cggcgtcggt gagtaaagag gaaacctctt ttgcaagcgt agtggcatcg gtaacggtaa
    28381 tcagcccgct tgcctgctcc agccgcgcgc aaatgtcttt aaagttaaac gtatgcggcc
    28441 ccatcaatac cggaatagcg tgtgcggccg cttccagcgg attatgccca ccacgttcaa
    28501 ccagtgaacc gccaacaaag gcgagatcgg caatgccgta cagtaacatc aactcgccca
    28561 tcgtatcgcc aaccacaacc tgcgtgctgg tggaggggac ttcccctgaa gatcgtgtga
    28621 tatagcttag tccagcctgg cggacaaggt taatcgcatc cgggaagcgt tctggatgac
    28681 ggggtaccag gatgagcaat aaattcggga attgctgtaa caatgcctga tgtgcggcga
    28741 tcaccacgct ctcttcgcct tcatgagtgc tggtggcaat ccataccggg cggtgtggtg
    28801 cccactggcg gcgcagcgtc acggctttag cagccaactg cggcgttaca gaaatatcga
    28861 atttcaggct accggtaacg gtcacctgat tattttttgc gcccagcgcc acaaaacgtg
    28921 caccatcttc ttcattttgg gcggcaatca gcgtaatacg acgcagcaag cggcggacga
    28981 atttacccag tttggcataa cctgcggccg agcgggcaga gagtcgcgcg ttagcgatca
    29041 ccagcggaat tttacgttta tgtagcgccg caatcaggtt aggccatagt tcggtttcca
    29101 taatcaacac cagtttaggg tcgactttat tcaggaaacg gttgagtgca tcgggcaggt
    29161 catacggcag ataaacgtgc tgaacatcct tcccgaaagc cgattgtacg cgctccgaac
    29221 cggttggagt catggttgtt acggtaatcg gtaaatcagg ataacgatga cgcagtgcgc
    29281 gcaccaacgg gatcgccgcc agagtttcac cgacggagac ggagtgcagc ataatgccgc
    29341 ctggttttag cggatggcgg taaaaaccgt aacgttcacc ccagcgtttt cgataggccg
    29401 gagccttacg tccgcgcacc cagagccgta tccagatcag cggctgaata aggtagagaa
    29461 gggcggtgta aagcaattcg agcatagtaa atagctgact tatggatgtg ctggggattc
    29521 tatgtattta gctgtggctt taccattact tttcccattt ttgacttaaa tagcaattta
    29581 cccgccaatt cttgctgaac atctatactt tttttgtatt tttatgcgat tcattgattt
    29641 ctgcagtacg tgcaggaagg aatcgcagag caacagtaaa agaatatggt tgctaatgat
    29701 tttatttgat tgatttcttt attgaaaatc aacaatgtgc ttaaaaatca ttcgagtcgg
    29761 attgccgcga aagaagtgat acactagcgc gtcaatatca gtgcggtatc agtattcagg
    29821 tagctgttga gcctggggcg gtagcgtgct ttttctgctt caccttaatc ggataatctc
    29881 aacaaaaaga gttacttgtg gataagccat ttcgaagaat tttgctcatt aagatgcgtt
    29941 ttcatgggga tatgttatta actactcccg tcattagttc gctgaaaaaa aattaccctg
    30001 acgcaaaaat cgatgtgctg ctttatcagg acactatccc gatcctgtct gaaaatccag
    30061 agattaacgc gctctacggc ataaaaaata aaaaagcaaa agcctcagaa aaaattacca
    30121 acttttttca tctcatcaag gtattacgtg ccaataagta tgaccttatc gttaatctta
    30181 ccgatcaatg gatggttgct atactggttc gcttattaaa tgcccgtgtg aaaatttccc
    30241 aggattatca tcatcggcag tctgcttttt ggcgtaatag tttcactcat ttggtgccgt
    30301 tgcagggtgg aaatgtggtg gaaagtaact tatccgtgct gacgccattg ggacttgaat
    30361 cgttggtgaa gcagacaacc atgagttacc cgcctgcaag ctggaaacgt atgcgtcgcg
    30421 aacttgatca cgctggtgtt ggacaaaatt atgtggttat ccaacctacg gcgcggcaaa
    30481 tcttcaaatg ctgggacaac gccaagtttt ccgctgtgat tgatgcctta catgctcgtg
    30541 gttatgaagt cgttctgacg tccggcccag ataaagacga tctggcctgc gtcaatgaaa
    30601 ttgcgcaggg atgccagacg ccaccagtaa cggcgctggc cggaaaggtg acctttccgg
    30661 aacttggtgc gttaatcgat catgcgcagc tgtttattgg cgttgattcc gcaccggcgc
    30721 atattgccgc tgcagttaat acgccgctga tatcgctgtt tggtgcgaca gaccatattt
    30781 tctggcgtcc ctggtcaaat aacatgattc aattctgggc gggagattac cgggaaatgc
    30841 caacgcgcga tcagcgtgac cgaaatgaga tgtatctttc ggttattccg gcggcagatg
    30901 tcattgcagc tgtcgataaa ttacttccct cctccacgac aggtacgtcg ttatgatcgt
    30961 tgctttttgt ttatataaat attttccctt tggcggtttg cagcgcgatt ttatgcgtat
    31021 tgctcagaca gtcgccgccc gaggtcatca tgttcgggtt tatacccagt cgtgggaagg
    31081 cgaatgccct gatgtatttg aactgatcaa agtgccggtt aaatcgcata ccaatcacgg
    31141 gcgcaatgcg gaatattttg cctgggtgca aaaacattta cgtgaacacc ccgtcgatag
    31201 agtcgttgga ttcaacaaaa tgccggggct ggacgtttat tatgccgctg atgtttgtta
    31261 tgccgagaaa gtagcgcagg agaaaggctt tttctatcgc ctgacgtcac gttatcgcca
    31321 ttatgccgcc tttgagcggg caaccttcga acagggcaag ccgacacagc tgctgatgct
    31381 gacagataag caaatcgccg atttccagaa acattatcag actgaagcgg agcgttttca
    31441 tattctgcca ccggggattt atcctgatcg taaatatagc cagcagccag ccaatagccg
    31501 tgaaatcttc cgtaagaaga atggaataac cgaacaacaa tatttattgt tgcaggtcgg
    31561 ttcagacttc acgcgtaaag gtgtcgatcg ttccattgaa gcacttgctt cgttaccgga
    31621 ttcgctgcgc cacaacacat tactatatgt tgttgggcag gataaaccgc gaaaatttga
    31681 ggcgctggca gaaaaacgcg gcgtgcgcag taatgttcac ttcttctcgg ggcgcaacga
    31741 tgtctcggaa ttaatggcgg cggcggattt attacttcat cctgcctacc aggaagccgc
    31801 aggaattgta ctgctggaag cgataactgc aggattaccg gtactaacaa cagccgtttg
    31861 tggctatgcg cattatattg tcgacgccaa ttgcggcgag gctattgctg agccattccg
    31921 ccaggaaaca ttgaatgaga ttttacgcaa agcgttaacg caatcttcat tgcgccaggc
    31981 ctgggcggaa aatgcgcgac attatgctga tacacaagat ttatacagtc tgccagagaa
    32041 agcggcggac atcataacgg gtggtctgga tggttgaact taaagagccg tttgccacgt
    32101 tatggcgcgg taaagatcct tttggggaag ttaaaacctt gcagggtgag gtatttcgtg
    32161 aactggaaac tcgccgcact ctgcgctttg aaatggcggg caaaagctat tttctcaaat
    32221 ggcatcgcgg cacgaccctg aaagagataa tcaaaaattt actctcattg cggatgccag
    32281 tattaggcgc agaccgcgaa tggaatgcga ttcatcgact gcgggatgtc ggcgttgata
    32341 ctatgtatgg ggtggcattc ggcgaaaaag gcattaatcc gctgaccaga acctcgttta
    32401 ttattaccga agatctgaca ccaaccatca gtctggaaga ttactgtgct gactgggcga
    32461 tcaacccacc agatgttcgc gtaaagcgta tgcttattaa gcgtgtcgcg acgatggtgc
    32521 gcgatatgca tgctgcgggc attaaccacc gtgactgtta tatctgtcat ttcctgctgc
    32581 acttgccttt ttccggtaag gaagaggagt taaaaatttc agtaattgac ctgcaccggg
    32641 cgcagatacg tgcaaaagta ccgcgtcgtt ggcgggataa agatttgatt ggtttgtatt
    32701 tttcatcaat gaatattggg ctgacgcaga gagatatctg gcgatttatg aaggtttatt
    32761 ttgggatgcc tttacgtgag atattatctt ttgaacggaa tttattgaac atggcaagtg
    32821 ttaaggccga gcatattaaa gagcgaacac aacgcaaagg attataaaaa tattaatcag
    32881 tatgtactgg aactaacttc attatgacta tttattttat aaactgggtt gcagattatg
    32941 aattaaaaat gatccagtac
//
LOCUS       DAZFJL010000039        32775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFJL010000039 DAZFJL010000000
VERSION     DAZFJL010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.039"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(99..3248)
                     /gene="acrB"
                     /locus_tag="ACHJ8C_001824"
     CDS             complement(99..3248)
                     /gene="acrB"
                     /locus_tag="ACHJ8C_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO5551690.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTNYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3271..4464)
                     /gene="acrA"
                     /locus_tag="ACHJ8C_001825"
     CDS             complement(3271..4464)
                     /gene="acrA"
                     /locus_tag="ACHJ8C_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO5551691.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4606..5253
                     /gene="acrR"
                     /locus_tag="ACHJ8C_001826"
     CDS             4606..5253
                     /gene="acrR"
                     /locus_tag="ACHJ8C_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO5551692.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5381..8743
                     /gene="mscK"
                     /locus_tag="ACHJ8C_001827"
     CDS             5381..8743
                     /gene="mscK"
                     /locus_tag="ACHJ8C_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO5551693.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQVQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATQNGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VSKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQTLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNATEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGSVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            8804..8902
                     /locus_tag="ACHJ8C_001828"
                     /pseudo
     CDS             8804..8902
                     /locus_tag="ACHJ8C_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8955..9116)
                     /gene="rsmS"
                     /locus_tag="ACHJ8C_001829"
     CDS             complement(8955..9116)
                     /gene="rsmS"
                     /locus_tag="ACHJ8C_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO5551694.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9130..9657)
                     /gene="priC"
                     /locus_tag="ACHJ8C_001830"
     CDS             complement(9130..9657)
                     /gene="priC"
                     /locus_tag="ACHJ8C_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO5551695.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARATALVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9727..10104
                     /gene="ybaN"
                     /locus_tag="ACHJ8C_001831"
     CDS             9727..10104
                     /gene="ybaN"
                     /locus_tag="ACHJ8C_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO5551696.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGAKPRAILLILITFTISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10257..10808
                     /gene="apt"
                     /locus_tag="ACHJ8C_001832"
     CDS             10257..10808
                     /gene="apt"
                     /locus_tag="ACHJ8C_001832"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO5551697.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10937..12868
                     /gene="dnaX"
                     /locus_tag="ACHJ8C_001833"
     CDS             10937..12868
                     /gene="dnaX"
                     /locus_tag="ACHJ8C_001833"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO5551698.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAAIRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEDSIRPI"
     misc_feature    12201..12265
                     /gene="dnaX"
                     /locus_tag="ACHJ8C_001833"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12921..13250
                     /gene="ybaB"
                     /locus_tag="ACHJ8C_001834"
     CDS             12921..13250
                     /gene="ybaB"
                     /locus_tag="ACHJ8C_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO5551699.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13250..13855
                     /gene="recR"
                     /locus_tag="ACHJ8C_001835"
     CDS             13250..13855
                     /gene="recR"
                     /locus_tag="ACHJ8C_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO5551700.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRKENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13965..15839
                     /gene="htpG"
                     /locus_tag="ACHJ8C_001836"
     CDS             13965..15839
                     /gene="htpG"
                     /locus_tag="ACHJ8C_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO5551701.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETIISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16020..16664
                     /gene="adk"
                     /locus_tag="ACHJ8C_001837"
     CDS             16020..16664
                     /gene="adk"
                     /locus_tag="ACHJ8C_001837"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO5551702.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRAALEKILG"
     gene            16796..17758
                     /gene="hemH"
                     /locus_tag="ACHJ8C_001838"
     CDS             16796..17758
                     /gene="hemH"
                     /locus_tag="ACHJ8C_001838"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO5551703.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQYSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALEMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATSEHIEMMANLVAAYR"
     gene            complement(17755..18714)
                     /gene="aes"
                     /locus_tag="ACHJ8C_001839"
     CDS             complement(17755..18714)
                     /gene="aes"
                     /locus_tag="ACHJ8C_001839"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO5551704.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNVGAPEMATRAYRVPTKYGQVKTRIFYPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALLDGAQFFTAQL"
     gene            18866..20170
                     /gene="gsk"
                     /locus_tag="ACHJ8C_001840"
     CDS             18866..20170
                     /gene="gsk"
                     /locus_tag="ACHJ8C_001840"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO5551705.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20300..21976)
                     /gene="ybaL"
                     /locus_tag="ACHJ8C_001841"
     CDS             complement(20300..21976)
                     /gene="ybaL"
                     /locus_tag="ACHJ8C_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO5551706.1"
                     /translation="MHHAPPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22215..23435)
                     /gene="fsr"
                     /locus_tag="ACHJ8C_001842"
     CDS             complement(22215..23435)
                     /gene="fsr"
                     /locus_tag="ACHJ8C_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO5551707.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23653..25305
                     /gene="ushA"
                     /locus_tag="ACHJ8C_001843"
     CDS             23653..25305
                     /gene="ushA"
                     /locus_tag="ACHJ8C_001843"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO5551708.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKFTVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25343..25846)
                     /locus_tag="ACHJ8C_001844"
     CDS             complement(25343..25846)
                     /locus_tag="ACHJ8C_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5551709.1"
                     /translation="MKFDHLVDVSFHNFVFNGKFDFVELGQTMAWLEQNFVEPDSKND
                     MGNGFYIWLFGNVEFHFENNKLYMIWCDYLSQIHLGKAIRFDKGILNNVAELNVTRVF
                     NMLVNEGADLQIRRQSPHTLLIHVNRSGVTLWFEADEEQPGDPQNYMLMAFGLTHRDY
                     DARFQYS"
     gene            complement(25843..26643)
                     /locus_tag="ACHJ8C_001845"
     CDS             complement(25843..26643)
                     /locus_tag="ACHJ8C_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5551710.1"
                     /translation="MERITETLSIACADEIPDIALLRQTSREKVLHLHFASEAERYRE
                     EFGLVLKAQLDEFVVGMHSADPVIRIARLISLEEINRHQDFFEHCAKEYRKLATELIF
                     ALAEQLNVEMAENNPLVTFAPFKCNRKRKGKMGKWQYCFHGFHCTFENKKTEQNIEVP
                     LAYGFDFGDLDPYFFSGFIKSTPAWQPLPVAIYDDYHDGSRIIQQLLALGKLQKIPSP
                     IPQYTGVAAVDRSNVDIANFRSTLESRLHRCKLRWLLKHVKNSQRSER"
     gene            complement(26667..27146)
                     /gene="ybaK"
                     /locus_tag="ACHJ8C_001846"
     CDS             complement(26667..27146)
                     /gene="ybaK"
                     /locus_tag="ACHJ8C_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO5551711.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAASDLAKILDAKFADIARRD"
     gene            complement(27350..28144)
                     /gene="ybaP"
                     /locus_tag="ACHJ8C_001847"
     CDS             complement(27350..28144)
                     /gene="ybaP"
                     /locus_tag="ACHJ8C_001847"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO5551712.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            28277..28579
                     /locus_tag="ACHJ8C_001848"
     CDS             28277..28579
                     /locus_tag="ACHJ8C_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5551713.1"
                     /translation="MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIIN
                     AATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYRLIFKWVNGKAEELFLDPHNY"
     gene            28615..28956
                     /gene="ybaQ"
                     /locus_tag="ACHJ8C_001849"
     CDS             28615..28956
                     /gene="ybaQ"
                     /locus_tag="ACHJ8C_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO5551714.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(29014..31518)
                     /gene="copA"
                     /locus_tag="ACHJ8C_001850"
     CDS             complement(29014..31518)
                     /gene="copA"
                     /locus_tag="ACHJ8C_001850"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO5551715.1"
                     /translation="MSQTIDLTLDGLSCGRCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALVVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     NVPLADVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADIDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVNTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            31780..32712
                     /gene="glsA"
                     /locus_tag="ACHJ8C_001851"
     CDS             31780..32712
                     /gene="glsA"
                     /locus_tag="ACHJ8C_001851"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO5551716.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKVGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENTEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTIELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
BASE COUNT         8089 a         8599 c         8513 g         7574 t
ORIGIN      
        1 tcgtatgaga tcctgagttg gtggttcaat actccttaat gttcgtaggt tatgcataaa
       61 aaaggccgct tgcgcggcct tagtgattac acgttgtatc aatgatgatc gacagtatgg
      121 ctgtgctcga tatcttcatt cttgcggcta aagcggcggc gaaccaccac aaagaatacc
      181 ggaacgaaga agattgccag taccgttgcg gtcaccatcc cgcccattac accggtacct
      241 actgcgttct gcgcgccgga accagcacca gtactgataa ccagcggcat aacgccgagg
      301 ataaacgcca acgaggtcat caggattggg cgtaaacgca tccgcaccgc atcaagcgtc
      361 gcttcaatca gacctttacc ttctttatcc atcaagtctt tggcgaattc gacgataagt
      421 atcgcgttct tcgccgacaa accaatggtt gtgagcaggc ctacctggaa gtaaacgtca
      481 ttggtcaggc cacggaaggt ggcagccagc aacgcaccga taacccccag cggaacgact
      541 agcataacgg agaacggaat cgaccagctc tcgtacagcg cagccagaca caggaacacg
      601 acaatcaacg aaatcgcgta cagtgaaggt gcctggttgc cggagagacg ttcctgatag
      661 gacattcccg tccagtcata gccaacaccg gtaggcagtt tgctcgccag ttgttccatc
      721 agctccattg cttcaccggt acttttaccc ggtgccgcct ggcctaagat ttccatagat
      781 ggcaggccgt tgtaacgttc cagacgcggc gaaccgtact cccaacgaga agaggagaac
      841 gccgagaacg gcaccatctg accatcagca gcacgaacat accagtcgcc gatatcatcc
      901 ggcagcatac ggtatttcgc ttctgacatg acgtaaactt tcttcacacg accgcggtcg
      961 ataaagtcgt tcacatagct accgccccat gcagcgccca gagtggtgtt aatgtcgttg
     1021 atagaaacac ccagcgcctg cgctttttcc tggtcgatat caatcttaaa ctgcggggta
     1081 tcttccagac cgtttggacg tacgctggtc aacatatcag ggtgcttcgc tgcttctgca
     1141 agcaactggt tacgcgcctg agtcagtttt tcgtgaccaa ggccagcctg gtcaatcagc
     1201 tcaaagtcaa agccggttgc ggtacccagt tccacgattg cgggcaggtt aaaggcgaaa
     1261 accatcgcat ctttgatttg cgagaaagca cgtgttgcac gcatggtaat cgcttcaact
     1321 ttgttttctt cgcccggacg atcggcccag tccttcaagg aaacgaacgc aataccggta
     1381 ttctgaccac gtcccgcaaa gccgaagccg ttaacggcga acaccgactc aacgttgttc
     1441 ttttctttgg tcagatagta attcgttacc tcattgagca ctttctgcgt acgttcctgc
     1501 gttgcacctg ctggcagctg aaccatggtc ataaatacgc cctggtcctc atctggcaag
     1561 aaggagcttg gcagacgcac gaacagatag gccataccga ccacgatgat cagatacagc
     1621 accaggtaac gccccgtact gcgcagaata ccgcctacgc tgtcggtgta gtggtgcgtg
     1681 ctcttctcga acatgcggtt aaaccagccg aagaagcctt ttttaccttc cccgtgatcg
     1741 cctttggcaa tcggtttcag catggtggca caaagagccg gagtcaggat caacgccacc
     1801 agtactgaca gggccattgc tgaaacaatg gtaatagaga actgacgata aatagcaccg
     1861 gtagaaccgc caaagaaggc catcggtacg aataccgccg acagtaccat cgcgataccg
     1921 accagagcgc cctgaatctg ccccatcgac ttacgggtag cttcttttgg cggcaaacct
     1981 tcttccgcca tcacacgctc aacgttttct accacaacga tggcgtcatc caccaacagg
     2041 ccgatggcga gcaccatccc gaacattgtt agcgtgttta tcgagaagcc aaaggcggca
     2101 aggacggcaa aggtccccag caataccacc ggtacggcaa tggtcggaat caacgtcgcg
     2161 cggaagttct gcaggaacag atacataacc aggaacacga ggatgatcgc ttcgaccagc
     2221 gttttaacca cttcgtgaat agagattttc acgaacggtg tggtgtcata cgggtaaaca
     2281 attttcagac ccgacgggaa gaacggttcc atcttcgcca gttcagcacg gattgccgca
     2341 gcggtatcca gcgcgttcgc accggtcgcc agcttgatcc ccagaccgga agccggttgg
     2401 ccgttaaact ctgcgatgat gtcgtagttc tcaccaccca gctcaatttt cgccacatca
     2461 cgcagcagca cgcgggaacc atcctgattc actttcagca ggattttgcc gaactcttca
     2521 gtagaggtca gacgcgtctg agcaataata gaggcgttaa gctgttggcc tttcaccggc
     2581 ggcgtaccac cgagctgacc agctgcaacc tgagcgttct gtgctttgat ggcggtaatg
     2641 acatcaaccg gcgttagctg gaatttgttc agttcattcg ggttcatcca gatacgcatc
     2701 gcgtactgtg aaccgaacaa ctgaacgtca cccacgcccg acgtacggct gatggcatct
     2761 ttcatattcg ccgccacgta gtcggagata tcctcctgcg tcatggtgcc atcggtgttg
     2821 ataacgccga caaccatcag gaagctgctg gatgatttct caacgctcac cccttgctgc
     2881 tgtacttctt gcggcagcaa cggcatcgcc agctgcagtt tgttctgcac ctgaacctgc
     2941 gcgatatccg catcagtacc agactcaaag gtcagggtga tctgcacggt acccgtggag
     3001 tcactgttag aggacatgta catcaggtta tcgataccgt tcatattctg ttcgataacc
     3061 tgtgtcaccg tgtcctgcac tgttttcgca tcagcgccag ggtaggaggc ggagatcgtt
     3121 actgccggcg gtgcaatcgt aggatattgc gccaccggca gtttgaggat cgccagcccc
     3181 cctgccaaca tgatgataat ggcgatcacc cacgcaaaaa tcgggcgatc gataaagaaa
     3241 ttaggcatgt cttaacggct cctgtttaag ttaagacttg gactgttcag gctgagcacc
     3301 gcttgcggct tgctggttat tatcagcggt aacttcttgt gcttttacct ggacaccagg
     3361 acgcactttc tgcagcccac ttattactac gcgatcgcct gctttcagac cttctgtcac
     3421 cagccactta tcgccaatag cctggcttgc aacgatcgga cgggtttcca ctttgtcatc
     3481 cgcgccaacc accagtacgg tggcatcgcc acgcggcgta cgggttacgc cctgttgcgg
     3541 gactaaaata gcgtttggat taagcccttc ttccagacgt gcgcgcacga acatacccgg
     3601 cagcagagtg tgatccgggt tcgggaagat agcgcgtagg gtgatagacc cagtggtctg
     3661 atcaacggta acgtcagaga attccagcgt accgtcctgc gggaacttaa tgccgtcact
     3721 ggtgatcagc gacactttgg ctttgccgtt ctcttgtttc agcgtgccat tcgccagttc
     3781 ctgtttcagg cgcaggaagt cgttgctgga ctgggtcaca tcaacgtaga tcggatcaag
     3841 ttgctgcacg gttgccagcg cagtcgcctg accgttctgt accaatgcgc cttccgtcac
     3901 gtttgactta ccaatgcgac cactaatcgg agaggtgact ttggtgtaag ccagattgat
     3961 tcgcgcagtt tcaacggcag ctttcgccgc agttaccgca gcattcgcct gttgcgcatc
     4021 agccagagcc tgatcgtact cttgcttact gatgtactga gtaccgagca atttctgata
     4081 acgattcacc gtcaattgcg cgatattggc tgcagcctgg gctttcgcca gatcaccttt
     4141 cgcactgtcg tatgccgcct gataggtcgc aggatcaatc tgatagagag agacacctgc
     4201 ttcgatgtcg ctaccttctt tgaaattacg cttcaggata atcccgctaa cttgaggacg
     4261 aacttctgcg atccggtagg cactggtgcg acccggaagc tcggttgtga tctgcagagg
     4321 ttcagttttg actgttacta cgccaacggc gggcatctgc tggccacctt gttgggcctg
     4381 tttgtcgtca catcctgtta gggctaagct gcctgagagc atcagaacga ccgccagagg
     4441 cgtaaaccct ctgtttttgt tcatatgtaa acctcgagtg tccgatttca aattggtcaa
     4501 tggtcaaaag ttaataaacc cattgctgcg tttatattat cgtcgtgcta tggtacatac
     4561 attcacaaat gtatgtaaat ctaacgcctg taaattcacg aacatatggc acgaaaaacc
     4621 aaacaagaag cgcaagaaac gcgccaacac atcctcgatg tggctctacg tcttttctca
     4681 cagcaggggg tatcatccac ctcgctgggt gagattgcaa aagcagctgg cgttacgcgc
     4741 ggtgcaatct actggcattt taaagacaag tcggatttgt tcagtgagat ctgggaactg
     4801 tcagaatcca atattggtga actcgagctt gagtatcagg caaaattccc tggcgatcca
     4861 ctctcagtat taagagagat attaattcat gttcttgagt ccacggtgac agaagaacgg
     4921 cgtcgattat tgatggagat tatattccac aaatgcgaat ttgtcggaga aatggctgtt
     4981 gtgcaacagg cacaacgtaa tctctgtctg gaaagttatg accgtataga acaaacgtta
     5041 aaacattgta ttgaagcgaa aatgttgcct gcggatttaa tgacgcgtcg cgcagcaatt
     5101 attatgcgcg gctatatttc cggcctgatg gaaaactggc tctttgcccc gcaatctttt
     5161 gatcttaaaa aagaagcccg cgattacgtc gccatcttac tggagatgta tctcctgtgc
     5221 cccacgcttc gtaaccctgc cactaacgaa taaccctgaa tctgactcca ggaattttcc
     5281 tggacatttt cgtcgttgct attctggttc actgcgtcgt gatattcttg cggtttgact
     5341 ttttcaggtc gttcttcagg ttcagaaacc ttcattcatc atgactatgt tccagtatta
     5401 caaacgatca cggcattttg ttttttcagc atttattgct tttgtttttg tcttgttatg
     5461 ccagaacacg gcgtttgcac gggcgtcatc gaatggtgat ttaccgacaa aagcggacct
     5521 gcaggcacaa cttgactcac tgaataaaca aaaagatctt tctgctcagg acaaactggt
     5581 gcagcaggat ctgacagata cattagccac cctcgataaa atcgatcgcg taaaagaaga
     5641 gacagttcag ctacggcaaa aagtcgctga agcgccggaa aaaatgcgcc aggcgaccgc
     5701 ggcgttaaca gcacttagtg atgtcgataa cgacgaagaa acgcgcaaaa ttctgagcac
     5761 gctttcgttg cgccagctgg aaactcgcgt tgcccaggcg ctggacgatt tgcaaaacgc
     5821 acaaaatgat ctggcgtctt ataacagcca actggtttcg ttacagacgc agcccgaacg
     5881 cgtgcaaaat gcgatgtata acgcttcgca gcagctgcaa caaattcgca gtcgtctgga
     5941 tgggactgat gtcggcgaga ctgccttacg tcccagccag aaagtgttaa tgcaggtcca
     6001 gcaggctttg ctgaatgccg agattgacca gcagcgtaaa agcctggaag ggaacactgt
     6061 cttgcaggat accttgcaaa agcagcgtga ttacgtgacg gcgaacagcg cccgtctgga
     6121 gcaccagtta caactgttgc aagaagcggt aaacagcaag cgcctgactt taaccgaaaa
     6181 aacggcgcag gaagctgtct ccccggatga agccgcgcgt attcaggcta atccgctggt
     6241 gaagcaggaa ctggaaatta accagcagtt aagtcagcgt ctgattaccg cgactcaaaa
     6301 cggtaatcag ttgatgcagc aaaacattaa agtcaaaaac tggctggagc gtgcgctgca
     6361 atcggaacgt aatattaagg agcagattgc cgttctgaag ggcagtctgc tgttgtcacg
     6421 tatcctgtac cagcaacaac aaacactccc ctcggcagat gaactggaaa acatgaccaa
     6481 ccgcatcgcg gatttgcgtc tcgaacagtt tgaagtcaac cagcagcgtg acgcgctgtt
     6541 ccagagcgat gcgttcgtca gcaaactgga agaaggtcac accaacgaag tcaacagcga
     6601 agttcacgat gcattattgc aagtagttga tatgcgtcgc gaattgctgg atcagcttaa
     6661 caaacagttg ggtaaccagt tgatgatggc cattaacctg caaatcaacc agcagcagtt
     6721 aatgagtgtg tcgaaaaacc tgaaatccat cctgacgcag caaatttttt gggtgaacag
     6781 taaccgcccg atggactggg actggattaa agcgttcccg caaacgctga aagatgaatt
     6841 taagtcgatg aaaatcacgg tgaactggga aaaagcctgg cccgccgtat tcatcgcctt
     6901 cctcgctggt ttgccgctgc tgttgattgc cgggctgatc cactggcgtc tgggctggct
     6961 gaaagcgtat caacaaaaac tggcttccgc tgtgggttcc ctgcgtaacg acagccagct
     7021 caacacacca aaagcgatcc ttatcgacct gatccgcgca ctgccggtgt gcctgattat
     7081 tctcgctgtc ggcctgattc tgctgactat gcagctcaat atcagcgagt tattgtggtc
     7141 gttcagcaaa aaactggcga ttttctggct ggtgtttggc ctgtgctgga aagtgctgga
     7201 gaaaaatggt gttgccgtgc gtcactttgg tatgccggaa cagcagacca gccactggcg
     7261 tcggcaaatt gtccgcatca gcctcgcgtt gctgccgatc catttctggt ctgtggtggc
     7321 agaactctcc ccgctgcatc tgatggatga tgtgctgggg caggcgatga ttttcttcaa
     7381 cctgctgctg attgccttcc tggtgtggcc gatgtgccgc gaaagctggc gtgataaaga
     7441 gtcgcacacc atgcgactgg tcaccattac cgtgctgtcg attatcccga ttgcgttgat
     7501 ggtgctgacc gccacgggtt acttctacac cacgctgcgc ctctccgggc gttggattga
     7561 aaccgtttat ttggtgatca tctggaacct gctgtaccag acggtattgc gcggcttaag
     7621 cgtagcggcg cggcgtatcg cctggcgtcg tgcactggcg cgtcggcaga atctggtgaa
     7681 agagggcgca gaaggtgccg aaccgccgga agaacccacc attgcgctgg agcaggttaa
     7741 ccagcagacg ctgcgtatta ccatgttgct gatgtttgcg ctgttcggtg tcatgttctg
     7801 ggcaatttgg tctgatttga tcaccgtgtt cagctatctc gacagcatta cgctctggca
     7861 ttacaacgct actgaagctg gtgctgcggt ggtgaaaaac gtcaccatgg gcagtctgtt
     7921 gtttgcgatt atcgcctcaa tggtggcctg ggcgttgatt cgcaacctgc ctggtttgct
     7981 ggaagtgctg gtgctctcgc gactgaatat gcgccagggc gcgtcgtatg ccattactac
     8041 catccttaac tacatcatta ttgccgttgg tgcgatgacg gtgttcggat cgctgggcgt
     8101 ctcgtgggat aaactccagt ggctggcggc agcattatcc gtaggtcttg gttttggttt
     8161 acaagagatc ttcggtaact tcgtttccgg tttgatcatt ctgttcgaac gtccggtgcg
     8221 cattggcgac acggtaacca ttggtagctt ctcgggttcg gtcagcaaga tccgtattcg
     8281 tgcgacaacg attaccgatt tcgatcgcaa agaagtgatt atcccgaaca aagcgtttgt
     8341 taccgagcgt ctgatcaact ggtcgttgac tgacacgact acgcgtctgg tgatccgtct
     8401 cggcgtagct tatggctccg atctggaaaa agtgcgtaaa gtgttgctga aggcggcgac
     8461 tgagcaccca agggtgatgc acgaaccaat gccggaagtc ttctttacgg catttggtgc
     8521 cagcacgttg gatcatgagc tgcgtctgta tgtgcgtgaa ctgcgtgacc gtagccgtac
     8581 tgtcgatgag ctgaaccgta ctatcgatca gttgtgccgt gaaaacgaca tcaacattgc
     8641 ctttaaccag cttgaagtgc atctgcacaa cgagaagggc gatgaagtga cggaagtgaa
     8701 acgcgactac aaaggcgacg atccgacacc agcggttggg taataaccaa agggggcgac
     8761 atttagttgc cccgagatta ctaacaaagt gcgcgttgtt catgccggat gcggcgtgac
     8821 cgccttatcc ggcctacgaa accgcaagaa ttcaatatat tgcaggagcg gtgtaggcct
     8881 gataagcgta gcgcatcagg cagttttgcg tttgcccaca atcttagggg acatttagcg
     8941 gccccattta tttctcactt ttccgcctca tcatcgcgcg ttaatttctt ttcataatcg
     9001 cgttttacaa tatccagcgc gcgcagtacg gtgcgggctg ggatctgatt ttcttccagc
     9061 agcacaatca aatcgacggc cagtttgaca tcgtcagggg cattttccag tgacatactc
     9121 tctccattgc tagcgggtta aacgcgctaa cctgttttcg attttttcca gcgcatggcg
     9181 gcagcgtgcc aggcgcgctt cataggcttc cacttcacga tgcagcgtct gctgttccac
     9241 gagagcggtc gctcgcgcca gacgggctct acgttcggca accatctcac gtagccgccg
     9301 ctcaaaatcc tgatgctgaa tacgtttacg ctgccagcgg gcaattttcg gtggtgcgct
     9361 atcccactcg cgtagcgacc aggctgtggc ttcacgcgct atagcctcca gttgcgccgc
     9421 cagatgttct gccagccagg cgacttgcgg cagctgttgc tgctcaaccg catgacgaag
     9481 cgcagccagg ttatcgcccg cctcatcgag acaagcctgt agtgttgtcg cgcgggtctg
     9541 aaaaaggtgc ctgtcaaaac gggcacttag cgtggcaaat tgcgctaccg gggcacaacg
     9601 ctgacgcagt gtggcgagct gtccttccag tttttccagc agcagggcgg ttttcacgat
     9661 aagacctcaa tgaaaatgat aattgttatg ctaaagtagc cactctgtaa gctgacaaca
     9721 ctcaatatgc aacgaatcat tttaatcatc attggctggc tggcggtagt gctgggtacg
     9781 ctgggcgtgg tattaccggt attaccgacg acgccgttta tcctgctggc ggcctggtgc
     9841 tttgcccgtt cttccccgcg ctttcacgcc tggttgctgt accgctcatg gtttggcagc
     9901 tatctacgtt tctggcagaa acatcatgcg atgccgcgcg gcgcaaaacc acgggcgatt
     9961 ttgcttattt tgattacgtt tacgatttcg ctgtggttcg tccagatgcc gtgggtgcgc
    10021 atcatgttgc tggtaattct cgcctgtttg cttttttata tgtggcgaat tccggtgatt
    10081 gatgaaaagc aagaaaagca ctgaagcaca acaatcgcag ttgcaattat tgcgtacagc
    10141 cagtacattc tggcgttttc gagcacaggc gcaggcggtc aaaggttaaa caactgttac
    10201 ttttgatacg ttcaaaacgc gccgtgagta ccaccgtaac aagcaggcat acacttatga
    10261 ccgcgactgc acagcagctt gagtatctca aaaatagcat caaaagcatt caggactacc
    10321 caaaacccgg cattcttttc cgcgatgtca ccagcttact ggaagacccg aaagcttacg
    10381 ctctcagcat cgacttgctg gttgagcgtt acaaaaatgc gggcattacc aaagtggtcg
    10441 gcaccgaagc gcgtggcttc ttgtttggcg ctccggtagc tctgggtctg ggcgttggct
    10501 ttgtaccggt ccgtaaaccg ggcaaactac cgcgtgaaac catcagtgaa acttacgacc
    10561 tggaatacgg cacagatcag ctggaaatcc acgttgatgc catcaaaccg ggcgacaaag
    10621 ttctggtggt ggacgatctg ctggcaaccg gcggcactat cgaagcgacg gttaaactga
    10681 tccgtcgtct gggtggtgaa gtagctgacg ctgcgttcat tatcaacctg ttcgatctcg
    10741 gcggcgaaca gcgtctcgaa aaacagggca ttaccagcta cagccttgtc ccgttcccgg
    10801 gccattaatt atagtcagtc ttacgctgcc cacgctacgg gtagcacaag atgtgcattc
    10861 agcctcgccg ttctgacggg gctgtgttag cattacccct tcgtgaatcc accttccagc
    10921 gtttcagagc ctgccaatga gttatcaggt cttagcccga aaatggcgcc cacaaacctt
    10981 tgctgacgtc gtcggccagg aacatgtgct gaccgcactg gcgaacggct tgtcgttagg
    11041 gcgtattcat catgcttatc ttttttccgg cacccgtggc gtcggaaaaa cctctatcgc
    11101 ccgactgttg gcgaaggggc taaactgcga aaccggcatt accgcgacgc cgtgcggcgt
    11161 gtgcgataac tgtcgtgaaa tcgagcaggg gcgctttgtc gatctgattg aaatcgacgc
    11221 cgcctcgcgc actaaagtcg aagatacccg cgacctgctg gataacgttc agtacgctcc
    11281 ggcgcgtggt cgtttcaaag tttatctgat cgacgaagtg catatgctgt cgcgtcacag
    11341 ctttaacgca ctgttaaaaa cccttgaaga gccgccagag cacgttaagt ttttgctggc
    11401 gacgaccgat ccgcagaaat tgccggtgac gattttgtca cgctgtctgc aatttcatct
    11461 caaggcgctg gatgtcgagc aaattcgcca tcagcttgag cacatcctca acgaagaaca
    11521 tatcgctcac gagccgcggg cgctgcaatt gttggcacgc gccgctgaag gcagcctgcg
    11581 agatgcctta agcctgactg accaggcgat tgccagcggt gacggccagg tttcaaccca
    11641 ggcggtcagt gcgatgctgg gtacgcttga cgacgatcag gcgttatcgc tggttgaagc
    11701 gatggtcgag gccaacggcg agcgcgtaat ggcgctaatt aatgaagctg ctgcccgtgg
    11761 tatcgagtgg gaagcgttgc tggtggaaat gctcggtctg ttgcatcgta ttgcgatggt
    11821 acaactttcg cctgctgctc ttggcaacga catggccgcc attgagctgc ggatgcgtga
    11881 actggcgcgc accataccgc cgacggatat tcagctttac tatcagacgc tgttgattgg
    11941 tcgcaaagaa ttaccgtatg cgccagaccg ccgtatgggt gttgagatga cgctgctgcg
    12001 cgcgctggcg ttccatccgc gtatgcctct gcctgagcca gaagtgccac gccagtcctt
    12061 tgcgcccgtt gcgccaacgg cagtaatgac gccaacacag gtgccgccgc aaccgcaatc
    12121 agcgccgcag caggcaccga ctgtaccgct cccggaaacc accagccagg tgctggcggc
    12181 gcgccagcag ttgcagcgcg tgcagggagc aaccaaagca aaaaagagtg aaccggcagc
    12241 cgctatccgc gcgcggccgg tgaataacgc tgcgctggaa agactggctt cggtcaccga
    12301 tcgcgttcag gcgcgcccgg tgccatcggc gctggaaaaa gcgccagcca aaaaagaagc
    12361 gtatcgttgg aaggcgacca ctccagtgat gcagcaaaaa gaagtggtcg ccacgccgaa
    12421 ggcgctgaaa aaagcgctgg aacatgaaaa aacgccggaa ctggcggcga agctggcggc
    12481 agaagccatt gagcgcgacc cgtgggcggc tcaggtgagt caactttcgc taccaaaact
    12541 ggtcgaacag gtggcgttaa atgcctggaa agaggagagc gacaacgcag tatgtctgca
    12601 tttgcgctcc tctcagcggc atttgaacaa ccgcggcgca cagcaaaaac tggctgaagc
    12661 gttgagcacg ttaaaaggtt caacggttga actgactatc gttgaagatg ataatcccgc
    12721 ggtgcgtacg ccgctggaat ggcgtcaggc gatatacgaa gaaaaacttg cgcaggcgcg
    12781 cgagtccatt attgcggata ataatattca gaccctgcgt cgattcttcg atgcggagct
    12841 ggatgaagac agtatccgcc ccatttgatc gtaagcacag cttacgttcg tcatccttaa
    12901 cgtgattgag agagaaacct atgtttggta aaggcggtct gggtaacctg atgaagcaag
    12961 cccagcagat gcaagaaaaa atgcagaaaa tgcaggaaga gatcgcgcag ctggaagtga
    13021 ccggcgaatc tggcgcaggt ctggtaaaag tgaccattaa cggtgcacac aactgccgtc
    13081 gcgtagagat cgacccgagc ctgctggaag acgacaaaga gatgctggaa gacctggtgg
    13141 ctgcagcatt caacgacgcg gcacgtcgta tcgaagaaac gcagaaagaa aaaatggcct
    13201 ctgtatcctc cggaatgcag ctgccgcctg gctttaagat gccgttctga tgcaaaccag
    13261 cccgctgtta acacagctta tggaagcact gcgctgtctg ccgggcgttg gcccgaagtc
    13321 ggcgcagcgt atggcgttca cgctgcttca gcgcgatcgt agcggcggga tgcgtctggc
    13381 gcaggcgctc acccgggcga tgtcggaaat cggccactgc gccgattgcc gcaccttcac
    13441 cgaacaggaa gtctgtaaca tctgttcgaa tccgcgtcgt aaggaaaacg gtcaaatctg
    13501 cgtggtggag agtccggcgg acatctacgc cattgagcag acggggcagt tttcaggccg
    13561 ttattttgtg ttgatggggc acctgtcacc gctggacggc atcggtccgg atgatatcgg
    13621 gcttgatcgt ctggaacagc gtctggcaga ggaaaaaatc actgaagtga tcctcgccac
    13681 caaccccacg gttgaaggtg aagctaccgc taactacatt gccgagcttt gtgcgcaata
    13741 tgacgtggaa gccagccgaa tcgctcatgg cgtaccggtt ggcggcgaac tggaaatggt
    13801 cgatggcacc acgctgtcac actcccttgc cgggcgtcat aagattcgtt tttaagcaaa
    13861 cgagagcagg atcacctgct ctcgcttgaa attactctcc cttgtcccca tctctccctc
    13921 atcctgtttt taaccttaaa atggcattat tgaggtagac ctacatgaaa ggacaagaaa
    13981 ctcgtggttt tcagtcagaa gtgaaacagc ttctgcacct gatgatccat tctctctatt
    14041 ccaataaaga aatcttcctg cgtgagctta tctctaacgc ctccgatgcg gcggacaagc
    14101 tgcgtttccg cgcgctttct aacccggacc tgtacgaagg tgatggtgaa ctacgcgttc
    14161 gtgtctcttt cgataaagac aagcgcacgc tgaccatctc cgataacggc gtggggatga
    14221 cccgcgacga agtgattgac catctgggga ctatcgctaa atccggcacc aaatcattcc
    14281 tcgaatccct gggttctgac caggcgaaag acagccagct gatcggtcag tttggtgttg
    14341 gtttctactc tgcgtttatc gtggctgaca aagtgaccgt gcgtactcgc gcggcaggcg
    14401 aaaaaccaga aaatggcgtc ttctgggaat cggctggcga aggtgaatac actgttgccg
    14461 acatcaccaa agaagatcgt ggtactgaaa tcaccctgca tctgcgtgaa ggcgaagacg
    14521 agttcctcga tgactggcgt gtgcgttcca tcatcagcaa atattccgac catatcgcgc
    14581 tgccggtaga gatcgaaaaa cgcgaagaga aagacggcga aaccattatc tcctgggaga
    14641 aaatcaacaa agcgcaggcg ctgtggactc gtaacaagtc ggaaatcacc gatgaagagt
    14701 acaaagagtt ctacaaacac atcgcccacg actttaacga tccgctgacc tggagccaca
    14761 accgcgttga aggtaagcag gagtacacca gcctgctgta cattccgtct caggcaccgt
    14821 gggatatgtg gaaccgcgat cataaacacg gtctgaaact gtatgttcag cgtgtgttca
    14881 tcatggacga cgcagaacag ttcatgccga actatctgcg cttcgtgcgt ggtctgattg
    14941 actccagcga tctgccgctg aacgtttccc gtgaaatcct ccaggacagc acggtaacgc
    15001 gtaacctgcg caatgcgctg accaagcgtg tgctgcaaat gctggaaaaa ctggcgaaag
    15061 acgacgcgga aaaataccag accttctggc aacagtttgg cctggtactg aaagaaggtc
    15121 cggcggaaga tttcgctaac caggaagcga tcgccaaact gctgcgtttt gcttctaccc
    15181 ataccgattc ttctgcgcag accgtgtctc tggaagacta cgtttcccgc atgaaagaag
    15241 ggcaggagaa aatctactat atcaccgcag acagttacgc ggcagcgaag agcagcccgc
    15301 acctggaact gctgcgtaag aaaggcatcg aagttctgct gctttccgac cgcatcgatg
    15361 agtggatgat gaactatctg actgagttcg acggtaaacc gttccagtct gtgtctaaag
    15421 ttgacgagtc gctggaaaaa ctggctgacg aagttgatga gagcgcgaaa gaagcggaga
    15481 aagcactgac tccgttcatc gaccgtgtga aagccctgct cggcgagcgc gtgaaagatg
    15541 tccgtctgac tcaccgtctg accgatacgc cagcgatcgt ttcgaccgac gcggacgaaa
    15601 tgagcactca gatggcgaaa ctgttcgctg cggcgggcca gaaagtgcca gaagtgaaat
    15661 acatcttcga actgaacccg gatcacgtac tggtgaaacg tgcggcggat actgaagatg
    15721 aagcgaagtt cagcgagtgg gtagaactgc tgctggatca ggcgctgctg gcagaacgcg
    15781 gtacgctgga agatccgaac ctgtttattc gtcgtatgaa ccagctgctg gtttcctgat
    15841 gtcatgccgg atgaccttcg tgtcatccgg catttttctt ttcttcatct gcactttccg
    15901 caaattatct cgccattaac cgtttcagcc tcaggtgcct ttcttgaggc aatcgcctgt
    15961 tggtggtatc gtttatcgct ttttcaaaaa attcgacaca ttttaagggg attttcgcaa
    16021 tgcgtatcat tctgcttggc gctccgggcg cggggaaagg gactcaggct cagttcatca
    16081 tggagaaata tggtattccg caaatctcca ctggcgatat gctgcgtgct gcggtcaaat
    16141 caggctccga gctgggtaaa caagcaaaag acattatgga tgctggcaaa ctggtcaccg
    16201 acgaactggt gatcgcgctg gttaaagagc gcattgctca ggaagactgc cgcaacggtt
    16261 tcctgttgga cggcttcccg cgtaccattc cgcaggcaga cgcgatgaaa gaagcgggca
    16321 tcaatgttga ttacgttctg gaattcgacg taccggacga actgattgtt gaccgtatcg
    16381 taggccgtcg cgttcacgcg ccgtctggtc gtgtttatca cgttaaattc aatccgccga
    16441 aagtagaagg caaagacgac gttaccggtg aagaactgac tacccgtaaa gacgatcagg
    16501 aagagaccgt acgtaaacgt ctggttgaat accatcagat gactgcaccg ctgatcggct
    16561 actactccaa agaagctgaa gcgggtaaca ccaaatacgc gaaagttgac ggcaccaagc
    16621 cggtggctga agttcgcgca gctctggaaa aaattctcgg ctaatcaagc cgcagcagat
    16681 ctgctttcgg gcaggtctgc tcatcctcct caggcaatac ctcttacagc aattagttct
    16741 tcttcttcac ttttccgcta caattatcaa caagttgaat cgataagagg cggtaatgcg
    16801 tcagactaaa accggtatcc tgctggcaaa cctgggcacg cccgatgccc ccacacctga
    16861 agcggtaaaa cgctatctga aacaattttt aagcgacaga cgcgtggttg atacctcacg
    16921 gttgttatgg tggccgttgc tgcgcggcgt gattttgccg ctgcgttcgc cgcgtgtggc
    16981 gaagctgtat gcctctgtct ggatggaagg tggctcgccg ctgatggttt acagccgcca
    17041 gcaacagcag gcgctggcgc aacgtttacc ggagacgccc gtggcgctgg gaatgagcta
    17101 cggctcgcca tcactggaaa gcgccgtaga tgaacttctg gcagagcatg tagatcatat
    17161 tgtggtgctg ccgctttatc cgcaatactc ctgttcaacg gtcggtgcgg tatgggatga
    17221 actggcgcga attctggcgc gcaaacgtag cattccgggg atatcgttta ttcgtgatta
    17281 cgccgataac cacgattata ttaatgcact ggcgaacagc gtacgtgctt cttttgccaa
    17341 acatggcgaa ccggatctgc tgttgctctc ttatcatggc attccccagc gttatgcaga
    17401 tgaaggcgat gattacccgc aacgttgccg cacaacaact cgcgaactcg cttccgcact
    17461 ggagatggca ccggaaaaag tgatgatgac ctttcagtcg cgctttggcc gggaaccctg
    17521 gctgatgcct tataccgacg aaacgctgaa aatgctcgga gaaaaaggcg tgggtcatat
    17581 tcaggtgatg tgcccgggct ttgctgcgga ttgtctggag acgctggaag agattgccga
    17641 gcaaaaccgt gaagtcttcc tcggtgcggg cgggaaaaaa tatgaatata ttccggcgct
    17701 taatgccacg tcggaacata tcgaaatgat ggctaatctt gttgccgcgt atcgctaaag
    17761 ctgagcggta aagaactgag cgccgtcgag aagagcctcg tcggcggttt tcatcatccg
    17821 tgaataatgc aaaaaggcgt gcagcgtgcc tgggtagagt ttgaactcac agggctgctg
    17881 atgcgccgct aacgtctggt aaagcagacg gctgtcatcc agcagtggat cgaactccgc
    17941 cccggcaata aaacagggcg gaacttcgcg agtgagatca ttattaaaca ggcagtaata
    18001 tggcgactcg cggtccgcgt cgttgcttaa atacgcctct tcatacatct gcaaatcctg
    18061 ttgcgttaag ccatcccaga caccacccaa cagacgacga gtcacggaat cccgtaatcc
    18121 gtaaagccca taccacagca aaacgcccgc aactttaccg caatcgatct gtttatcgcg
    18181 caaccacaac gcactggcga gcgccagcat ggcacctgcg gaatcaccgg caaaaccaat
    18241 gcgggacata ttgatttgat aatcctccgc ctgctggtgg aaataacaac aggcagccac
    18301 aatttcctct atcgcttgcg gaaaacgcgc ttcaggtgaa agggtgtaat caatgccaat
    18361 caccgtacat tggctgtagc ttgccagcag gcgcatgatg cgatcgtggg tatcgagatt
    18421 gccgagaata aaaccgcctc catgcaaata aaatagcgtc gccgggctat ctggctgcgg
    18481 ataaaagata cgtgttttca cctgcccata ttttgttgga accctgtaag ctctggttgc
    18541 catttctgga gcgcccacat tccagaatcg gcgctcaagc gtgtaatact gtcgttgctc
    18601 agcaatcgtt cccgttgcag gccagggcgg taaatccggc tgaagagtat tcacaacggt
    18661 cttcatttca gcagagataa ggtccagaac gggtaattta ttttccggct tcatgcaaaa
    18721 actcctttca aattacgtca ttgtaaggaa ccactgccat gaaaatgcga tcccgcctgc
    18781 tgatattgaa actggctgcg tctcgcgcgc tcccgtcaga ttgtgttaac attcgccgct
    18841 cagttaacca cccgtaaaaa caaccatgaa atttcccggt aaacgtaaat ccaaacatta
    18901 cttccccgta aacgcacgcg atccgctgct tcagcaattc cagccagaaa acgaaaccag
    18961 cgctgcctgg gtagtgggta tcgatcaaac gctggtcgat attgaagcga aagtggatga
    19021 tgaattcatt gagcgttatg gattaagcgc cgggcattca ctggtgattg aggatgacgt
    19081 tgccgaagcg ctttatcagg aactaaaaca gaaaaacctg attacccatc agtttgcggg
    19141 tggcaccatt ggtaacacca tgcacaacta ctcggtgctc gcggacgacc gttcggtgct
    19201 gctgggcgtc atgtgcagca atattgaaat tggcagctac gcctatcgtt acctgtgtaa
    19261 cacctccagc cgtaccgatc ttaactatct acaaggcgtg gatggcccga ttggtcgttg
    19321 ctttacgctg attggcgagt ccggggaacg tacctttgct atcagcccgg gccacatgaa
    19381 ccagttgcgg gctgaaagta ttccggaaga tgtgattgcc ggagcctcgg ctctggttct
    19441 cacctcttat ctggtgcgtt gcaagccggg tgaacccatg ccggaagcaa ctatgaaagc
    19501 cattgagtac gcgaagaaat ataacgtacc ggtggtgctg acgctgggca ccaagtttgt
    19561 cattgccgag aatccgcagt ggtggcagca attcctcaaa gaccacgtct ctatccttgc
    19621 gatgaacgaa gatgaagccg aagcgttgac cggagaaagc gatccgttgt tggcatctga
    19681 caaggcgctg gactgggtag atctggtgct gtgcaccgcc gggccaatcg gcttgtatat
    19741 ggcgggcttt accgaagacg aagcgaaacg taaaacccag catccgctgc tgccgggtgc
    19801 tatagcggaa tttaaccagt atgagtttag ccgcgccatg cgccacaagg attgccagaa
    19861 tccgctgcgt gtctattcgc acattgcgcc gtatatgggc gggccggaaa aaatcatgaa
    19921 cactaacgga gcaggggatg gcgcactggc agcgttgctg catgacatta ccgccaacag
    19981 ctaccaccgt agcaacgtac caaactccag caaacataaa ttcacctggt taacttattc
    20041 atcgttagcg caggtgtgta aatatgctaa ccgtgtgagc tatcaggtac tgaaccagca
    20101 ttcacctcgt ttaacgcgcg gcttgccgga gcgtgaagac agcctggaag agtcttactg
    20161 ggatcgttaa gttatcgtcg gttcgcaggc cagataaggc gttcacgccg catctggcat
    20221 tttgctttca atgcctgatg cgacgctggc gcgtcttatc aggcctacat atccgcgcct
    20281 attatccggc aaccaccgtt taccccgtca ccacctcacc cgccggtggc gtttccagca
    20341 gttccagcat agtacgggca atttcacgct cacccatcac tacctgattc gcaccacgtt
    20401 cggtgatata cgccacttca tcgtcataat gggcgcgggc aataatctca atatccggat
    20461 ttttcgcgcg ggcagatgcc acaatctcgc ccgcttcata gccgttggga atcgtcagga
    20521 tcagccattt tgcacattcc agatgcgcca gttgcataat ttcttcgttc gccgcattgc
    20581 ccaatactgc gcggaccccg cgctcgcgca gctcatcaac acgggttcgt gacgtctcaa
    20641 tcaccaccag cggaatatca gaggcgagca atttctcccc cagcaggctg cctacacgac
    20701 cgtaacccac cagtagcgca tggttgcaaa tatccactgg gatctgcttc tcttcttcga
    20761 ttgcctcttc cagcgtctgc tcttccagcg tttcggtctt cgccagatat ttctccagta
    20821 gtgcgaacag taccgggttg agcataatcg acaggatcgc ccctgccagt accaggtttt
    20881 gtccggcctg cggcagtaaa ttcaatgcca ttcctagtcc cgccaggata aacgcgaact
    20941 caccaatctg cgccaggctg gcggcgatgg ttaacgccgt gcgctgggag tgaccaaaca
    21001 aacgcacaag gaacagggcc gcgaccgatt taccaaacag aataattgcc agcgtcgcca
    21061 gtactgccag cggttgctga atcagaatta acggatcaaa caacatcccg acggagacaa
    21121 aaaacagcac cgcaaacgcg tcgcgcaatg gcagtgtatc gtgggcggca cggtgactca
    21181 gttcagactc gttcagtacc atcccggcaa agaacgcacc gagtgcaaag gagacatcaa
    21241 acagctctac cgcaccaaag gcaatcccta acgccagcgc cagcaccgac agggtaaaca
    21301 gctcgcggga accggttgct gcgctgcgtg ccataatcca cggcaccaga cggcgaccta
    21361 ccagcatcat aatggcgata aatgcgatca ccttgccgat ggtgatcccc atatcgactg
    21421 caagagtggc aaagcccaca tcgccctgtt ccatcattcc tgctaccgcg ggtagcaata
    21481 ccagtgtcag gaccatcacc aggtcttcca caatcaacca accgatggcg atttgcccac
    21541 gctgactgtc aattaattgc cgttcttcaa gtgcgcgcag taacaccacg gtactggcgg
    21601 tggaaagaca taagccgaac acgataccgg tcattaacga ccagcccagc acggcggaga
    21661 gcgccatacc cagcagcgtc gccacggcta tctgggcgat cgcgccggga atggcgatgg
    21721 actttaccgc catcaaatcc ttgagcgaaa aatgcaaacc gacgccgaac atcagcaaaa
    21781 taacgcccag ttcagccagt tccggcgcaa gcttggtatc ggcaacaaag cccggagtga
    21841 atggtcctgc cagcacaccc gctaacagat atcccaccag aggagaaata cgtagtttat
    21901 tggccagcat gccgaggata aaggcgagca caaggccgcc aacaatggtg gtgataagcg
    21961 ggggggcgtg atgcattccg tctccttttc ctggtggtta ttgtccattt ttggccggga
    22021 aaaccaaaat tacaggtaat agtttatgac aatttcattg atgatgttta tgaataattg
    22081 ttgaattttg cagaaaaatg gaattagctg caaaaaaaag cacggatcgg aaaacagaag
    22141 gcggtttaac agaggaaagg cttatggcga ctgtaaaacg atgcagccaa agtttgcctt
    22201 tggctgcaat gaaatcagtc tttatgccgg ttatcaggca ggaatatggt caacatcccc
    22261 aatagtggca ggaaagcaca gattttatag actaactcta tgctggtgtg gtcggcgata
    22321 agccccagaa ctgccgcccc cagacctccc atgccgaaag caaaaccgaa aaatagtcca
    22381 gaaaccatac cgatacgtcc cggaagcagc tcctgggcat agaccagaat ggcagagaat
    22441 gccgaagcga ggataaatcc aataatcacc gttaaaaccc ccgtccagtg caggctggcg
    22501 tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag agccccaaat cacatatttc
    22561 cgtccaattt tatcccctac aggcccgcca atcaccgtac ctgccgcaac ggcaaacagg
    22621 aaggcaaaca aatgaagctg agcattctgg atagataatc cgaatttttg catcagataa
    22681 aaggtgtaat agctgctgat gctcgccata tagaaatatt tcgagaaaat gaggattaac
    22741 agaatgctga ccgccagaac aactttattg cgcggcagtg gattgataat cgtcgctttg
    22801 ggttttcctt tattcattcg gtgctgtgcc gagtaccaac ggctgatttg cgccaacacc
    22861 acgatcgcca gcagtgccgc aagcacaaac caggcaacgt tgcctttgcc ataaggcgcg
    22921 ataatcaccg ccgccagcaa ggggcccagg gaactgccaa agttgccgcc gacctgaaag
    22981 atagattgcg ccaggccatg acgcccgccg gaagccatac gggccacgcg agaagattcc
    23041 ggatgaaaga ccgatgaacc ggtaccgacc agcgccgctg ccagcagaac tgcgccgaaa
    23101 ctgcccgcca gcgccagcag caccagacca cttaaggtaa agcacatgcc aatcggcaac
    23161 gaccacggca tcggatattt atcggtccag tagccgacca ccggttgcag tagcgaagag
    23221 gcgagctgga aggtgagggt tatcatgcca atctgcataa atgtcagaga aaattctgac
    23281 tgaagcagtg gataaatcgc cagaatcagc gattggatca tgtcgttcag cagatgtgag
    23341 aggctgatag cacctaaaat accaaacgat gttcgggcct tagtcgttga cgcagccgcg
    23401 cccgccacag gctgggtttg ttcactcatt gccataggaa agtcactttt tcagggttgc
    23461 gatgtaaaga atgatcttat ttgtgattat tacccgacta acatacctgt atgcgccgcc
    23521 tgaaggaagt ctcaacgcgg aatacagaat ttctaatctg gatgcagatt tatcttcacc
    23581 ggacgcagac ttgtctatga tgtcgcgtca tactattttt caacacgttg aaatcaggtc
    23641 agggagagaa gtatgaaatt attacagcgg ggcgtggcgt tagcactgtt aaccacattt
    23701 acactggcga gtgaaactgc tctggcgtac gagcaggata aaacctacaa atttacagtt
    23761 ctgcatacca atgatcatca tgggcatttt tggcgcaatg aatatggcga atatggtctg
    23821 gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg ttgcggctga aggcggtagc
    23881 gtgctgctac tttccggtgg cgacattaac actggcgtgc ccgagtccga cttacaggat
    23941 gccgaacctg attttcgcgg tatgaatctg gtgggctatg acgcgatggc gataggtaat
    24001 catgaatttg ataacccgct caccgtatta cgccagcagg aaaagtgggc taagttcccg
    24061 ttgctttccg ccaatatcta ccagaaaagt accggcgagc gcctgtttaa accatgggcg
    24121 ctgtttaagc gtcaggatct gaaaattgcc gttattggcc tgacgacgga tgacacagcc
    24181 aaaattggta atccggaata tttcactgat atcgaatttc gtaagcccgc tgatgaagcg
    24241 aagctggtga ttcaggagct gcaacagaca gaaaagccag acattattat cgcggcgacc
    24301 catatggggc attacgacaa tggtgagcac ggctctaacg caccgggcga tgtggagatg
    24361 gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg gtggtcactc gcaagatccg
    24421 gtctgtatgg cggcagaaaa caaaaaacag gtcgattacg tgccgggtac gccatgcaaa
    24481 ccggatcaac aaaacggcat ctggattgtg caggcgcatg agtggggcaa atacgtggga
    24541 cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg ttaactacca gctgattccg
    24601 gtgaacctga agaagaaagt gacctgggaa gacgggaaaa gcgagcgcgt gctgtacacc
    24661 cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat caccgttcca gaacaaaggc
    24721 aaagcgcagc tggaagtgaa aataggcgaa accaatggtc gtctggaagg cgatcgtgac
    24781 aaagtacgtt ttgtacagac caatatgggg cggttgattc tggcagcaca aatggatcgc
    24841 actggtgccg actttgcggt gatgagcgga ggaggaattc gtgattctat cgaagctggc
    24901 gatatcagct ataaaaacgt gctgaaagtg cagccattcg gcaatgtggt ggtgtatgcc
    24961 gacatgaccg gtaaagaggt gattgattac ctgacagccg tcgcacaaat gaagccagat
    25021 tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg cgaaagacgg caaactgaac
    25081 gacctgaaaa tcaaaggcga accggtcgat ccggcgaaaa cttaccgtat ggcgacatta
    25141 aacttcaatg ccaccggcgg tgatggctat ccgcgccttg ataacaaacc gggctatgtg
    25201 aataccggct ttattgatgc cgaagtgctg aaagcgtata tacagaaaag ctcgccgctg
    25261 gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc agtaatgaac cccgtcgcgg
    25321 cgtcatgata tgacgccgcc atttatgaat attgaaatct tgcatcgtaa tcgcgatgtg
    25381 tcaaaccaaa tgccatcagc atataattct gcggatcacc aggctgttct tcatctgctt
    25441 caaaccaaag tgtcacgcct gagcgattga cgtggattaa gagtgtatgg ggcgattgac
    25501 ggcggatttg cagatcagcg ccttcgttaa caagcatgtt aaatacacgc gtaacgttca
    25561 actcagcgac gttattcaat attcctttat caaatcggat cgctttgccc aaatggattt
    25621 ggctcagata atcgcaccaa atcatatata gtttgttgtt ttcgaaatga aactcaacat
    25681 taccaaatag ccagatataa aaaccatttc ccatatcatt tttagagtcc ggctcaacaa
    25741 agttttgttc caaccatgcc attgtttgcc ccagttcgac aaaatcgaat tttccgttaa
    25801 agacaaaatt gtggaagcta acatcaacaa gatggtcaaa tttcatcttt cgctcctttg
    25861 tgaatttttt acatgcttca aaagccagcg taatttacat cggtgcaggc gactctccag
    25921 tgttgagcga aaatttgcga tgtcgacatt ggaccgatct accgcagcaa cccccgtgta
    25981 ttgtgggatc ggtgaaggga ttttttgtaa ctttcccagc gccagcagtt gttgaatgat
    26041 cctgctaccg tcgtggtaat catcgtaaat agccactggc agaggctgcc aggcgggggt
    26101 cgatttgata aatccggaaa aaaaatacgg gtccagatcg ccgaaatcaa agccgtaggc
    26161 gaggggaact tcaatatttt gctctgtttt tttattctca aatgtacaat gaaaaccgtg
    26221 aaaacaatac tgccactttc ccattttgcc ttttcgctta cggttgcatt tgaagggggc
    26281 aaaggtaact agcgggttat tttccgccat ctcaacgttc agttgttcag ccaatgcaaa
    26341 tataagctct gtcgccagct tacgatattc tttcgcgcag tgttcgaaaa aatcctgatg
    26401 gcggttaatc tcttcaagcg atattaatct ggcgatcctg ataaccgggt cggcagaatg
    26461 catgccaaca acaaattcat cgagctgtgc tttcagcacc agcccgaatt cctcacgata
    26521 cctctctgct tcgctggcaa aatgcagatg taataccttc tctctcgatg tttgccgaag
    26581 taaggcgata tccggtatct catctgcaca agcgatactt aatgtctctg taatcctttc
    26641 cacatctcat cttccgtgtg catcacttaa tcgcgacggg cgatatcagc aaatttggca
    26701 tcgagtatct ttgccaggtc gcttgccgcc agttcgatat ccagaccgcg cttgccgccg
    26761 gaaacataaa tagtggcaaa ttcttgtgcg ggggcgtcga taatcgttgg cagacgtttt
    26821 ttctgcccca gtgggctaat ccctccaacc agatatcccg tcgaacgctg cgcgaccatc
    26881 ggatcggcca tctcaacttt cttggcaccc agcgcttttg ctactttttt aagatccagt
    26941 tgacctgcga ccggcgtaac ggccacggca aggtgtttca tatcaccgtt cactgcgacc
    27001 agcagcgttt tgtagacctg atccggattc aaacctaatt ttttgacgac ttcatcgcca
    27061 aaattggttt cagccggatc gtgctcgtag gtatggattt gaaacgaaat cttgtttttt
    27121 tcgagtaatt taactgcggg tgtcatggta attttcctgc cttaaacaaa aaaacgaagc
    27181 aagcatacgc ctgatggcgg tccaaaaaaa agagtcatct tgcctaagag tattggcagg
    27241 atggtgagat tgagcgacaa tcgagttaca ccgtcgctta aagtgacggc ataataataa
    27301 aaaaatgaaa ttcctctttg acgggccaat agcgatattg gccatttttt tagcgcaaca
    27361 tctgcggcaa attcccttct ccatacaggt gtagtgcacc gactgcgacc acatagcgcc
    27421 ccggcggcat ggcgcgtaat ttatcccgcc aggcgagatt tcgctgatgc atcagcacat
    27481 cgtacagcga ctgactgaac gtattgggca gcgttatttc attattttgc ggcggcgcat
    27541 tcaaccacca gctcatcatt tgttgcagca accgtgcatt ggtgtgccag tgggtcagcg
    27601 tgtcgtccag cagcgccagt cctttgtcag ggagctggag caacatggca atctggtttt
    27661 cagccccttc cagttcaatc acgggtttat gttgttgctt cgccgcctgc aatagctggt
    27721 aatcgatacc gtattctgcc cgcagcccca gtttttgtgc ctgcgtcgcc tgaagaacca
    27781 tcgcgatttg ccacaacggt tgggtagaaa agagcgacgg agaaatgccc atctcctggc
    27841 taatgtgctg caggttttgg agttgctcct cgctaatgcg ctcttccagt gcctcgcagg
    27901 caggcaaatt ggcaaaaggc gtatcgctgg tggaaacatc cgcctcgacg atcagcgcat
    27961 cggcgttttt gagctttttg agcaaacggg tgggcagggg agccatatcg tggctaccca
    28021 tatgaatact gccaatcaga tgaaaatggc gattgccggg aagagtgata tcgatagcag
    28081 gccaggtgta atgattaccg cgcagcgcgg cccaaagcgt ttttacccgg tacaacagat
    28141 ccatacgacc tccctttgtg aaatatcatg ctagcgcgcg gggagggatg gcgcaaatgg
    28201 agttgattgt tgcacttgcg cacagtgctt gtgtgcatta caatgattga ataatggaca
    28261 aggagatgag agaggaatgg ctcaaaagaa aaatataagg agttttcgtg atgcctggtt
    28321 ggctgatttt tttgtccatt caacgccaca tcgaaagatc cctgctgaaa tccataccac
    28381 gttatcacga aagttggaca tcattaatgc tgccacttct cattgggatt tacgctcccc
    28441 tccggggaac cgatacgaag agttatccgg gaaattacag gaatactcct ccattagagt
    28501 gaataaacag taccgactga tttttaaatg ggttaatggc aaggccgaag aattgttttt
    28561 agacccacac aactactaaa acatagctgt cgtattgacg agaaaggtaa aactatgaaa
    28621 caggcaacaa gaaaaccgac gacacccgga gatattcttc tctatgaata tctggaaccg
    28681 ctcgatttga aaatcaatga gttagcagag ttgctgcatg ttcatcgtaa tagcgtcagt
    28741 gcactgatca ataacaatcg taaactcacc actgagatgg cattccgtct ggcgaaagtt
    28801 tttgatacca cagtcgattt ttggctaaac ctccaggcgg cagttgatct ttgggaagtt
    28861 gaaaacaaca tgcgtaccca ggaagaattg ggacggattg aaacagtggc tgaatatttg
    28921 gcacgccgtg aagagcgtgc aaaaaaggtc gcgtagaata atttccctat aacggatgcg
    28981 ccaccctaaa gcagcgcatc cgcaatgatg tacttattcc ttcggtttaa accgcagcaa
    29041 gcggttggcg ttactcacta cggtgatcga cgagagcgcc attgccgctc cggcaaccac
    29101 cgggttaagc agtgttccag tgaacggcca caaaataccg gcggcgaccg gaataccaat
    29161 gctgttgtag ataaacgcac cgagcaggtt ctgcttcatg ttgcgcagcg ttgcgcggga
    29221 aattgcgagc gcatccgcaa cacccatcag gctatggcgc atcagggtaa tcgccgcggt
    29281 ttcaatggca acatcactgc cgccacccat cgcaataccg acatccgcct gagccagcgc
    29341 tggcgcatcg ttaatgccgt cgcccaccat tgccacctga cgtccttcac tttgcagacg
    29401 tttgatcgct tcggctttac cgtccggcag cacgccggct atcacctcat caatccctgc
    29461 ttctttagcg atcgcattgg cggtggtcgg gttatccccg gttagcatca ccaggcgata
    29521 tcccgcttta tgcaggcgtt gcagcgccgc cacgctatca ctacgcaacg gatcgcgtac
    29581 agccagcagg gctaccgctt tcccgtcaac cgccagcagc acaggcgttg ccccttgcga
    29641 tgcctgagct gaaatatctg cttcaattgc tttggtattg acctgttggt catttaacag
    29701 cgcctgattg cccagcaata acgcatgacc ttcagcttca ccgctcacgc ccagcccgcg
    29761 caatgtgcgg aaaccgttga cctgcggtag ctgcatatca cctgctttat cgaggatcgc
    29821 ccgtgccaga gggtggctgg aaccttgctc cagcgccgcc gccagacgca atgcttgcgc
    29881 ttcatcaata tcagcaaatg tttttactgc gacaacctgc ggcttccctt cggtcagcgt
    29941 cccggtttta tcgaacacta cggtgtcgag cgtactggcg cgttgcagcg cgtcggcgtc
    30001 ccgtaccagc acgccaaact cagccgcccg cccgacgccg gaaataatcg acatcggcgt
    30061 cgccagtccc agcgcacacg gacaggcaat aatcagcacc gtggtggcaa tcaccagggt
    30121 atagacaatc tgcggtgccg gaccaaagaa ataccagatt gccgcactga tcagcgcaat
    30181 caccaccact accggcacaa atacggctga gattttatcc gccagttggc cgatttcagg
    30241 cttgctgctc tgggcctggc gcaccatgcg aatgatccgt gacagcgtag tatggctgcc
    30301 gaccgcacta gcacgaaaca gcacgctacc gtcctgcacc actgtcccgg catggacgct
    30361 atcgccttcg cctttttgct gcgggatagg ttcgcccgtc agcatcgctt catccagcca
    30421 cgcttcgcct tgagtaatct caccatctac cggcacacgg tcgccggtcg tcaggcgcag
    30481 taacatacct ggctgaacat ctgccagcgg cacgtttttt tcaccttcgt cggtgaccag
    30541 gcgcgccgtc ggtggggtta aatcgagtaa tttctccagc gccttagaag aacgctgacg
    30601 cgcgcgcgct tccagcatat ggccgagatt gatcagaccg ataatcatcg cgctggcttc
    30661 gtaataaaga tgtcgcgctt ccatcgggaa ccactgcggc cacaggttga cgctcatcga
    30721 atagagccac gccacgccag tacccagcgc caccagcgta tccatcgtcg ctgcaccgtt
    30781 cagcaggctt ttccatgcac tgcggtaaaa atggccgccg gcgaaaacca tcactgccag
    30841 ggttatcagg ccgataacca accacaggct gcggttgtca gcggtgacca tcatgttatc
    30901 gccgatcatc ccccagacca tcaccgggat acccaccacc agcgcgacaa ttgcctgcca
    30961 gcggaagcgc ttcatcgtag cgacggcggt ttcttgctgg cgctcgcggc gtttagcgtc
    31021 atcttcaatc gcttccgcgc cgtagcccgc tttttccacc gcctgcacta aatcttgtgg
    31081 ggaggcactg cccatcacca gcgcagtacg ctccgccagg tttacccgtg cctgagtgac
    31141 gcccggtacg ctttgcaacg cattttgtac gcgggtgaca cagctggcgc agctcatgcc
    31201 gctcagcagc aactgctggc tgtcatcgtc atcggcggtc gctgccggaa gcgcctcaga
    31261 aaccgctgtc agtgcttccg acgggattga tgactccgcc agcggtttag cctttgggtg
    31321 gcttacagat gcgtcataac ccgcttgttt gatggtttcg atcagctgtt ctgcactggc
    31381 agtcccggta acgtgcgctt cagtgataga cacatccgcc tgttcaacat ccgggcgctg
    31441 ttcgagactt tctttcacgc gtttaacgca gcgaccgcag gacaggccgt ccagggtcag
    31501 gtcgatagtt tgtgacataa aacactcctt taagacagtt ttgactggct gtgataaagg
    31561 ttaaaccttc cagcaagggg aaggtcaaga aattaataaa ccaggcgggt aaaagtccgt
    31621 aaagattaaa aaatcggctc gatttgcatc aggattagac atttatctct ttgttttcct
    31681 gtagttaagt tgcgggtgct aagttaaatc aggatgcatg aaaatcggca ccggggtgag
    31741 gaattacctc ccgcatctat aaaaaggagt taacaaaaga tgttagatgc aaacaaatta
    31801 cagcaggcag tggatcaggc ttacacccaa tttcactcac ttaacggcgg acaaaatgcc
    31861 gattacattc cctttctggc gaatgtccct ggtcaactgg cggcagtggc tatcgtgacc
    31921 agcgatggca acgtctatag tgcgggtgac agtgattacc gctttgcgct ggaatccatc
    31981 tcgaaagtct gtacgttagc ccttgcgtta gaagatgtcg gcccgcaggc ggtacaggac
    32041 aaagttggcg ctgacccgac cggattgccc tttaactcag ttatcgcctt agagttgcat
    32101 ggcggcaaac cgctttcgcc actggtaaat gctggcgcta ttgccaccac cagcctgatt
    32161 aacgctgaaa atactgaaca acgctggcag cgaattttac atatccaaca acaactggct
    32221 ggtgagcagg tagcgctctc tgatgaagtc aaccagtcgg aacaaacaac caacttccat
    32281 aaccgggcca ttgcctggct gctgtactcc gccggatatc tctattgtga tgcaatggaa
    32341 gcctgtgacg tgtacacccg tcagtgctcc acgctcatca atactattga actggcaacg
    32401 cttggcgcga cgctggcggc gggtggtgtg aatccgctga cgcataaacg cgttcttcag
    32461 gccgacaatg tgccgtacat tctggccgaa atgatgatgg aagggctgta tggtcgctcc
    32521 ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg gtgtaggtgg cggtattctg
    32581 gcggtcgttc ctggcgtgat gggaattgcc gcgttttcac caccgctgga cgaagaaggc
    32641 aacagtgttc gcggtcaaaa aatggtggca tcggtcgcta agcaactcgg ctataacgtg
    32701 tttaagggct gatcatgatg aacacggaag gtaataacgg taacaaaccc ctcggtctat
    32761 ggaacgtcgt ttcca
//
LOCUS       DAZFJL010000040        32749 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFJL010000040 DAZFJL010000000
VERSION     DAZFJL010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.040"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..327)
                     /gene="tccP2"
                     /locus_tag="ACHJ8C_001852"
     CDS             complement(<1..327)
                     /gene="tccP2"
                     /locus_tag="ACHJ8C_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
                     /protein_id="HFO5551717.1"
                     /translation="MINSINSFFSGLPRSISSAIRSSTFTVSQHKSTPNTVKTSSPFS
                     PSNSPASATTIFKVKNSYTESGLQRPTSYTQSSIEKNALHRPLPDVAQRLVQHLAEHG
                     IQPARNM"
     gene            complement(930..1017)
                     /locus_tag="ACHJ8C_001853"
     tRNA            complement(930..1017)
                     /locus_tag="ACHJ8C_001853"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(981..983),aa:Ser,seq:tga)
     gene            1444..2562
                     /gene="hyaA"
                     /locus_tag="ACHJ8C_001854"
     CDS             1444..2562
                     /gene="hyaA"
                     /locus_tag="ACHJ8C_001854"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO5551718.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            2559..4352
                     /gene="hyaB"
                     /locus_tag="ACHJ8C_001855"
     CDS             2559..4352
                     /gene="hyaB"
                     /locus_tag="ACHJ8C_001855"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO5551719.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFSEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALKLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            4371..5078
                     /gene="hyaC"
                     /locus_tag="ACHJ8C_001856"
     CDS             4371..5078
                     /gene="hyaC"
                     /locus_tag="ACHJ8C_001856"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO5551720.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            5075..5662
                     /gene="hyaD"
                     /locus_tag="ACHJ8C_001857"
     CDS             5075..5662
                     /gene="hyaD"
                     /locus_tag="ACHJ8C_001857"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO5551721.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEDVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALVQLAAWGIVP
                     QPTEELRCLNYDCLSMENYEGVRLRQYRMRLEEQG"
     gene            5659..6057
                     /gene="hyaE"
                     /locus_tag="ACHJ8C_001858"
     CDS             5659..6057
                     /gene="hyaE"
                     /locus_tag="ACHJ8C_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO5551722.1"
                     /translation="MSNDTPFNALWQRMLARGWTPVSECRLDDWITQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            6054..6911
                     /gene="hyaF"
                     /locus_tag="ACHJ8C_001859"
     CDS             6054..6911
                     /gene="hyaF"
                     /locus_tag="ACHJ8C_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO5551723.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCRVNGEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRHGEQLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINMTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            7045..8589
                     /gene="appC"
                     /locus_tag="ACHJ8C_001860"
     CDS             7045..8589
                     /gene="appC"
                     /locus_tag="ACHJ8C_001860"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO5551724.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            8601..9737
                     /gene="appB"
                     /locus_tag="ACHJ8C_001861"
     CDS             8601..9737
                     /gene="appB"
                     /locus_tag="ACHJ8C_001861"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO5551725.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            9750..9842
                     /gene="cbdX"
                     /locus_tag="ACHJ8C_001862"
     CDS             9750..9842
                     /gene="cbdX"
                     /locus_tag="ACHJ8C_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO5551726.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            9922..11220
                     /gene="appA"
                     /locus_tag="ACHJ8C_001863"
     CDS             9922..11220
                     /gene="appA"
                     /locus_tag="ACHJ8C_001863"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO5551727.1"
                     /translation="MKAILIPFLSLLIPLTPKSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLTKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNRKKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(11335..13515)
                     /gene="etk"
                     /locus_tag="ACHJ8C_001864"
     CDS             complement(11335..13515)
                     /gene="etk"
                     /locus_tag="ACHJ8C_001864"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO5551728.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKAGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITHILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PQVRSELDQAEEKLNAYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLASVIAQSDQKVLFI
                     DADLRRGYSHNLFNVSNEHGLSEYLAGKDEPNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRYVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(13535..13981)
                     /gene="etp"
                     /locus_tag="ACHJ8C_001865"
                     /gene_synonym="yccY"
     CDS             complement(13535..13981)
                     /gene="etp"
                     /locus_tag="ACHJ8C_001865"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO5551729.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(13969..15108)
                     /gene="gfcE"
                     /locus_tag="ACHJ8C_001866"
     CDS             complement(13969..15108)
                     /gene="gfcE"
                     /locus_tag="ACHJ8C_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO5551730.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTITGVHDLTETSRWIQTWPN"
     gene            complement(15154..17250)
                     /gene="gfcD"
                     /locus_tag="ACHJ8C_001867"
     CDS             complement(15154..17250)
                     /gene="gfcD"
                     /locus_tag="ACHJ8C_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO5551731.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAANWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(17250..17996)
                     /gene="gfcC"
                     /locus_tag="ACHJ8C_001868"
     CDS             complement(17250..17996)
                     /gene="gfcC"
                     /locus_tag="ACHJ8C_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO5551732.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGMVTIYLPGEQQTLSVGPVEN
                     VAQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWLAGRSVTDYLQDHTRLAGADKNNVMVITPEGEAVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(17993..18643)
                     /gene="gfcB"
                     /locus_tag="ACHJ8C_001869"
     CDS             complement(17993..18643)
                     /gene="gfcB"
                     /locus_tag="ACHJ8C_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO5551733.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNN
                     LLEVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGR
                     DVLCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(18750..19055)
                     /locus_tag="ACHJ8C_001870"
     CDS             complement(18750..19055)
                     /locus_tag="ACHJ8C_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5551734.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATAVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(19497..19709)
                     /gene="cspH"
                     /locus_tag="ACHJ8C_001871"
     CDS             complement(19497..19709)
                     /gene="cspH"
                     /locus_tag="ACHJ8C_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO5551735.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            19995..20207
                     /gene="cspG"
                     /locus_tag="ACHJ8C_001872"
     CDS             19995..20207
                     /gene="cspG"
                     /locus_tag="ACHJ8C_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO5551736.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            20218..20406
                     /gene="ymcF"
                     /locus_tag="ACHJ8C_001873"
     CDS             20218..20406
                     /gene="ymcF"
                     /locus_tag="ACHJ8C_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO5551737.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            20381..20611
                     /gene="ymcE"
                     /locus_tag="ACHJ8C_001874"
     CDS             20381..20611
                     /gene="ymcE"
                     /locus_tag="ACHJ8C_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO5551738.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            20601..20774
                     /gene="gnsA"
                     /locus_tag="ACHJ8C_001875"
     CDS             20601..20774
                     /gene="gnsA"
                     /locus_tag="ACHJ8C_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO5551739.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(20823..21896)
                     /gene="yccM"
                     /locus_tag="ACHJ8C_001876"
     CDS             complement(20823..21896)
                     /gene="yccM"
                     /locus_tag="ACHJ8C_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO5551740.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAINI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVSFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISMIVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKSCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVMGIMFAAIGYAMYAG
                     VWQSPVPEELYQRLIPQSPMIGH"
     gene            complement(21968..24711)
                     /gene="torS"
                     /locus_tag="ACHJ8C_001877"
                     /pseudo
     CDS             complement(21968..24711)
                     /gene="torS"
                     /locus_tag="ACHJ8C_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            24794..25822
                     /gene="torT"
                     /locus_tag="ACHJ8C_001878"
     CDS             24794..25822
                     /gene="torT"
                     /locus_tag="ACHJ8C_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO5551741.1"
                     /translation="MRVLLLLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQKAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGNPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVMTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(25795..26487)
                     /gene="torR"
                     /locus_tag="ACHJ8C_001879"
     CDS             complement(25795..26487)
                     /gene="torR"
                     /locus_tag="ACHJ8C_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO5551742.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            26617..27789
                     /gene="torC"
                     /locus_tag="ACHJ8C_001880"
     CDS             26617..27789
                     /gene="torC"
                     /locus_tag="ACHJ8C_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO5551743.1"
                     /translation="MRKLWNALRRPSARWSVLALVATGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            27789..30335
                     /gene="torA"
                     /locus_tag="ACHJ8C_001881"
     CDS             27789..30335
                     /gene="torA"
                     /locus_tag="ACHJ8C_001881"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO5551744.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFISPQDASARGIRHGDV
                     VRVFNVRGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            30332..30931
                     /gene="torD"
                     /locus_tag="ACHJ8C_001882"
     CDS             30332..30931
                     /gene="torD"
                     /locus_tag="ACHJ8C_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO5551745.1"
                     /translation="MTMLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNGLENSIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLDLLSHLHFSLGEGTVPARRIDGLRQKTLTA
                     LREWLPEFAARCRQYDSFGFYAALSQLLQVLVECDNQKG"
     gene            complement(31288..31593)
                     /gene="cbpM"
                     /locus_tag="ACHJ8C_001883"
     CDS             complement(31288..31593)
                     /gene="cbpM"
                     /locus_tag="ACHJ8C_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO5551746.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPSEIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(31593..32513)
                     /gene="cbpA"
                     /locus_tag="ACHJ8C_001884"
     CDS             complement(31593..32513)
                     /gene="cbpA"
                     /locus_tag="ACHJ8C_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO5551747.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFEPRKDWGKA"
     gene            32666..32749
                     /locus_tag="ACHJ8C_001885"
                     /pseudo
     CDS             32666..32749
                     /locus_tag="ACHJ8C_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7738 a         8304 c         8677 g         8030 t
ORIGIN      
        1 catattccgt gctggttgaa tgccatgctc tgcaagatgc tgcaccagac gctgagccac
       61 atcaggtaga ggccgatgta aagcgttttt ttctatactt gactgagtat aagatgtggg
      121 acgttgaagt cctgattctg tataagaatt ttttacctta aaaatagttg ttgcactggc
      181 aggactgttg ctgggagaaa aaggtgagct ggtttttacc gtgttaggtg tgcttttgtg
      241 ctgcgagaca gtgaaggtac tgctgcgtat agcacttgat atactgcgag gaaggccgga
      301 aaaaaaagaa ttaatgctat ttatcatata gataacctta taagagcttt attggatagt
      361 cttatttatc cgtttacatg aaatctacct tttgtgaaat tatcgtcaat attttactca
      421 aaatgatcgg ttgtaggaat aaactatttg atattattac gtttttagag ttgttttcaa
      481 tcaactatag atagggcttg gcgccagaat ggagaatatt taacgaggaa tagaagcttt
      541 gtatcagacg ggcttcccta aatagagaag ttaaataatc aggatgatgc taatgaatat
      601 cagggacaag gtgagtgtct tcatgttcag tgaaacaggg tgtcacaaaa aagattgggg
      661 agggctagag ccttctgcac gaaaatagtg atgcgacaga tctgagatgg ctttgtatcc
      721 ttctgcctgg ttcaggcctg gagcagtcca tgttttacaa aaatactcgg cagcgtaacg
      781 tgttgcaaga gcggcattag acgaggaacc tgaggttccg ctcatgaccg ataatctcat
      841 ccgaataaaa atataccaat tggtatacca agtttatacc aagagccaaa aaagcaaagg
      901 ggctaccatt tggtaacccc ttgattattt ggcggaagcg cagagattcg aactctggaa
      961 ccctttcggg tcgccggttt tcaagaccgg tgccttcaac cgctcggcca cacttccgga
     1021 atgacgcgca ctataaacat cccgatgcgg cgtgtaaacc cctaatttgt ttgtttgcct
     1081 gaaaaacagc caaaagtgca ttgatagcgt gaaataacag cagattgatc atttcatcac
     1141 catgaattcc ttctctttta ctcgtttagc aaccggctaa acatccccac cgcccggtaa
     1201 aaagaaaaat aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt
     1261 gtgcaaaagt ttcactacgc tttattaaca atactttctg gcgacgtgcg ccagtgcaga
     1321 aggatgagct ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac
     1381 gtcgcttgcc gaccatgtgc gcccggtgtc ctgccggtgt cgcaaggaga agagacgtgc
     1441 gatatgaata acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc
     1501 agctttctca aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca
     1561 ccaaagattg cctgggcgct ggagaacaaa ccgcgcattc cggtggtgtg gatccacggt
     1621 cttgaatgca cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac
     1681 gtcatccttt ccctgatttc cctcgattac gacgatactt tgatggctgc cgccggaacc
     1741 caggcggaag aagtctttga agacatcatc acgcaataca atggcaaata tatcctcgcc
     1801 gttgaaggta atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg
     1861 tttattgaga aactcaaacg tgccgctgcc ggagccagtg cgattatcgc ctggggaacc
     1921 tgcgcgtcct ggggctgcgt gcaggccgcg cgacccaatc cgacgcaggc aacgcctatc
     1981 gacaaagtca tcaccgacaa acctattatc aaagtacctg gctgcccgcc gatcccggat
     2041 gtgatgagcg ccatcattac ttacatggtg acctttgatc gtctgccaga tgtcgacagg
     2101 atgggccgtc cgttgatgtt ctatggtcag cgtatccacg ataaatgcta tcgccgtgct
     2161 cacttcgacg ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttac
     2221 tgcctgtaca aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc
     2281 tggaacgatg gcgtttcctt cccgatccag tcaggtcacg gctgcctggg ttgtgcggaa
     2341 aatggtttct gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc acaaatgggt
     2401 acccattcca ccgccgacac cgtcggttta accgcgcttg gcgtggtggc agcggctgtt
     2461 ggtgtgcacg cagtcgccag cgccgttgac cagcgcagac gtcataacca gcaacctaca
     2521 gaaaccgaac atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc
     2581 agggatacac catcaacaat gccggacgcc gcctggtggt cgacccgatt acgcgcattg
     2641 aaggccacat gcgctgcgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct
     2701 cctgcggcac catgtttcgc gggctggaga tcatcctgca agggcgtgac ccgcgcgatg
     2761 cgtgggcgtt cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg
     2821 tttacgccat cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca
     2881 acattatgct ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg
     2941 ggatggactg gatcgatgtg ttagatgcgc tgaaagccga cccgcggaaa acctccgaac
     3001 tggcgcaaag cctctcctca tggccgaaat catcccctgg ctatttcttc gacgtacaaa
     3061 accgcctgaa gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg
     3121 ggcacccgca gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg
     3181 aagctctcga tttccagcgt gaaattgtca aaatccacgc ggtctttggt ggtaaaaacc
     3241 cgcatccaaa ctggattgtc ggcgggatgc cttgcgccat taacattgac gaaagcggcg
     3301 cagtcggggc ggtcaatatg gaacgcctga acctggtgca gtcgatcatt acccgtacgg
     3361 cggacttcat taacaacgtg atgatccccg acgccttagc catcggccag ttcaacaaac
     3421 cgtggagcga aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc
     3481 cggatattgc caacgacttt agtgagaaaa gtctgctgat gcctggcggc gcggtgatta
     3541 acggcgactt caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt
     3601 ttgtcgacca cgcctggtat cgttatccca acgatcaggt ggggcgtcat ccgttcgatg
     3661 gcattaccga cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc
     3721 agctgaatga acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga
     3781 tggaagtggg gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg
     3841 ttgagtcggt cgatcgtatg atgtcggcgt tgaaactgcc gctttccggc atccagtcaa
     3901 cgttgggccg cattttgtgt cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt
     3961 atttcttcga caagctgatg accaacctga aaaacggcaa tcttgccacc gcttccacgg
     4021 aaaaatggga accagcaacc tggccgacag agtgccgtgg tgtcggtttt accgaagcgc
     4081 cgcgcggggc gttaggccac tgggctgcca ttcgcgatgg caagattgat ctctaccagt
     4141 gcgtggtgcc gaccacctgg aacgccagcc cgcgcgatcc caaagggcag attggcgctt
     4201 atgaagcggc gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatcctgc
     4261 gtactctgca cagctttgac ccgtgcctcg cctgttcaac acacgtgctg ggcgacgacg
     4321 gtagcgagct tatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga
     4381 aaagcgacaa cgttgtcagt cactatgtct ttgaagcacc cgtgcggata tggcactggc
     4441 tgacagtgtt atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac
     4501 cttccgtcag cggcgaggcg acgtatctgt tctatatggg ctacatcagg ttaattcact
     4561 tcagcgccgg gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg
     4621 gcaatcgtta ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg
     4681 gcgtgtggta tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag
     4741 gccataaccc catcgcccag gcggcgatgt tcggctattt cctgatgtca gtttttatga
     4801 tcatcaccgg ctttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc
     4861 gttatgtggt ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc
     4921 atcggctggg gatgtggctg attggcgcgt ttgtgatcgg tcatgtctac atggcgctgc
     4981 gtgaagacat catgtccgac gacacggtga tctccactat ggtcaacggc taccgtagcc
     5041 acaaatttgg caaaataagt aacaaggagc gttcatgagc gagcaacgcg tggtggtaat
     5101 ggggctgggc aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct
     5161 gtatgcccat taccactggc ccgaggatgt ggagattgtc gatggcggca cccaaggact
     5221 gaacttgctg gggtatgtcg aaagcgccag tcatctgttg attctcgatg ccattgatta
     5281 cgggctggaa cccggaacgc tacgaacgta tgccggagaa cgcattccgg cttatctcag
     5341 cgcgaagaaa atgagcctgc accagaacag tttctccgaa gtgctggcgc tggcagatat
     5401 ccgcggacat ctgccagcac atattgccct cgtcggtctg caacccgcga tgctcgacga
     5461 ctacggcggc agcctgagcg aactggcgcg ggagcaactg cccgctgcgg aacaggcggc
     5521 gctggtacag ctggcggcct ggggaattgt accgcaaccg actgaggagt tgcgctgtct
     5581 caattatgac tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgtc agtacaggat
     5641 gagactggag gaacagggat gagcaacgac acaccattta atgcgttgtg gcaacgaatg
     5701 ctggcgcgtg gctggacgcc ggtcagtgaa tgccgtcttg acgactggat tacgcaagcg
     5761 ccagacggcg tggtgttatt aagcagtgac ccgaaacgca cgccagaagt cagcgataac
     5821 ccggtaatga ttggcgaatt actgcgcgag tttcccgact atacatggca ggtagcgatt
     5881 gctgatcttg agcagagcga agccatcggc gatcgctttg gcgtctttcg ctttcccgcc
     5941 acattagtgt ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg
     6001 gaactgataa acctgatgcg cgggcttgtc gaaccgcaac aggagcgtgc ttcatgagcg
     6061 aaactttttt ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga
     6121 atccactgcc gatcacctgt cgggtgaatg gtgaaccgag tatggctgcc ctggagcaat
     6181 gtgctcacag cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac
     6241 gccagccgcc gctgggtgag gtactggcag tcgatctgtt aaatctcaac gccgacgatc
     6301 gtcactttat caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg
     6361 acgacagtga aagtgaaatt caggaggcga tcttctgcgg attatggcgg gtacgcagac
     6421 gtcacggcga acagttgctg gaagacaaac tggaggctgg ctgcgcaccg ctggcgttgt
     6481 ggcaggcggc aacgcaaaac gtcttgccga cagattcgct gttaccgccg cccattgatg
     6541 gcctgatgaa tggcctaccg ttggcccatg aattactggc acatgtacgt aaccccgacg
     6601 cgcagccgca cagcattaat atgacgcaat tacccatcag cgaagctgac cggctttttc
     6661 tctcacgtct ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct
     6721 atatcaacgc cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag
     6781 gcccgttact ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag
     6841 aagatttggt cgactctgcg cagcggctta gcgaggtatg ccagtggctg gcggaggggg
     6901 caccgacgta aaaagacggt aagtatcgct ttcaggctta tgaatatcac aatcggcgaa
     6961 tacctctggt cgtagagttt caggataaag agggagatct accattaacg ggttattttt
     7021 ctctcttcgc ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt
     7081 gcactgaccg cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg
     7141 ctggctatca tggaaaccat ttacgtggtc accggcaaaa caatctaccg cgatatgacg
     7201 cgcttctggg gtaagctctt cggtatcaac tttgctcttg gcgtggctac cggcctgacc
     7261 atggagtttc agtttggtac taactggtca ttctattcca actatgtggg cgatattttt
     7321 ggcgcaccgc tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg
     7381 ctgttcttct tcggctggca gcggctgaat aaataccagc acctgctggt gacgtggctg
     7441 gtggcgttcg gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa
     7501 tacccgaccg gtgcgcattt tgatatcgac accctgcgta tggagatgac cagtttcagc
     7561 gagctggtct ttaacccggt cagccaggtg aaatttgtgc acaccgtaat ggcgggatac
     7621 gtgaccgggg ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag
     7681 cgcgatgtcg cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc
     7741 ggtacgctgc aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa
     7801 ctggcggcga tggaagggga gtggcaaacg gaacctgcac ctgcaccgtt ccatgtggtt
     7861 gcctggccgg aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta
     7921 gggatcctcg ccactcattc attagataaa cccgtgccgg gtctgaagaa tttgatggct
     7981 gaaacctacc cacgcttgca acgcggacgt atggcgtggc tgttaatgca ggaaatatcg
     8041 caaggcaatc gtgagccgca tgtgttgcag gcattccggg agctggaagg cgacctgggt
     8101 tacggcatgt tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac
     8161 caggcggcga tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc
     8221 atggtgggct gtggttccct gctgctactg gtgatgctga ttgcgcttgt tcagacgctg
     8281 cgtggcaaaa tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgctg
     8341 ccgtggattg cgattgaagc cggatggttt atgaccgagt ttggtcgtca gccgtgggcg
     8401 atacaggaca tcttaccgac atactccgcg cactccgcct taaccacagg acaactggct
     8461 ttctcactga tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg
     8521 atgcagaaat atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa
     8581 caggggtaaa ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc
     8641 tgattggcgt gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct
     8701 gtctgctgcc gctggtggcg cgtaatgatg atgaacgccg gatagtgata aacagcgttg
     8761 gcgcgcactg ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgccg
     8821 cctggcccag agtgtatgca gcggcgtttt ccggctttta tgtggcgatg attctggtgc
     8881 tgtgctcgct gttcttccgc ccgctggcct ttgattatcg cggaaaaatc gccgatgcac
     8941 gctggcgtaa aatgtgggat gccggactgg tcatcggcag tctggtgccg ccggtagtct
     9001 tcggtattgc attcggcaac ttgctgctcg gcgtgccgtt tgctttcaca ccgcaattac
     9061 gcgtggaata tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggat
     9121 tgctcagtct tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg
     9181 gtgtgattca tctgcgttca cagctggcga ccaaacgcgc cgcactactg gtgatgctgt
     9241 gctttttgct ggcgggttac tggctgtggg ttggtattga tggctttgta ctgctcgccc
     9301 aggatgctaa cggtccttcc aatccgttaa tgaaactggt ggcagtgcta cctggtgcct
     9361 ggatgaataa tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct
     9421 gcccattgct gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg
     9481 cgtcattgat gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca
     9541 tgccgtcaag cgtgagtccg atctccagcc tgacgttgtg ggacagcact tccagtcagc
     9601 tgacgctgag cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca
     9661 ctctctggag ctactacaaa atgtgggggc gcatgacgac agaaactctc cgccgtaacg
     9721 aaaacgagct gtactaagga gcaggaacaa tgtggtattt actttggttc gtcggcattt
     9781 tgttgatgtg ttcgctctcc acccttgtgt tggtatggct ggacccgcga ttgaaaagtt
     9841 aacgaaggta agcctgatga ggcgcattag cgtcgcatca ggcaatcaat aatgtcggat
     9901 atgaaaagcg gaaacatatc gatgaaagcg atcttgatcc cattcttatc tcttctgatt
     9961 ccgttaacac cgaaatctgc attcgctcag agtgagccgg agctgaagct ggaaagtgtg
    10021 gtgattgtca gtcgtcacgg tgtgcgtgca ccaaccaagg ccacgcaact gatgcaggat
    10081 gtcaccccag acgcatggcc aacctggccg gtaaaactgg gttggctgac accgcgcggt
    10141 ggtgagttaa tcgcttatct cggacattac caacgccagc gtctggtagc cgacggattg
    10201 ttgacgaaaa agggctgccc gcagcctggt caggtcgcga ttattgctga tgtcgacgag
    10261 cgtacccgta aaacaggcga agccttcgcc gccgggctgg cacctgactg tgcaataacc
    10321 gtacataccc aggcagatac ctccagtccc gatccgttat ttaatcctct aaaaactggc
    10381 gtttgccaac tggataacgc gaacgtgact gacgcgatcc tcagcagggc aggagggtca
    10441 attgctgact ttaccgggca tcggcaaacg gcgtttcgtg aactggaacg ggtgcttaat
    10501 ttcccgcaat caaacttgtg ccttaaccgt aagaaacagg acgaaagctg ttcattaacg
    10561 caggcattac catcggaact caaggtgagc gccgacaatg tctcattaac cggtgcggta
    10621 agcctcgcat caatgctgac ggagatattt ctcctgcaac aagcacaggg aatgccggag
    10681 ccggggtggg gaaggatcac tgattcacac cagtggaaca ccttgctaag tttgcataac
    10741 gcgcaatttt atttactaca acgcacgcca gaggttgccc gcagtcgcgc caccccgtta
    10801 ttggatttga tcatggcagc gttgacgccc catccaccgc aaaaacaggc gtatggtgtg
    10861 acattaccca cttcagtgct gtttattgcc ggacacgata ctaatctggc aaatctcggc
    10921 ggcgcactgg agctcaactg gacgcttcca ggtcagccgg ataacacgcc gccaggtggt
    10981 gaactggtgt ttgaacgctg gcgccggctc agcgataaca gccagtggat tcaggtttcg
    11041 ttggtcttcc agactttaca gcagatgcgt gataaaacgc cgctgtcatt aaatacgcct
    11101 cccggagagg tgaaactgac cctggcagga tgtgaagagc gaaatgcgca gggcatgtgt
    11161 tcgttggcag gttttacgca aatcgtgaat gaagcacgca taccggcgtg cagtttgtaa
    11221 tgcataaaaa agagcattca gttacctgaa tgctctgagg ttgatgacaa acgaagaact
    11281 gtctgatgcg taggccggaa aaggcgttca cgccgcatcc ggccactttc agttttactc
    11341 tttctcggag taactataac cgtaatagtt atagccgtaa ctgtaagcgg tgctggcgcg
    11401 tttaatcaca ccattgagga tagcgccttt aatattgacg cctgcctgtt ccagacgctg
    11461 cattgacaag ctcacctctt tggcggtgtt caggccaaaa cgcgcaacca gcaggctggt
    11521 gccgacatag cgacccacga ccgcagcatc gctcactgcc agcatcggcg gcgtatcgac
    11581 aattaccaga tcgtaatggt cgttcgccca ttccagtaat tgacgcatcc gatcgcgcat
    11641 cagcagttca gacgggttag gtggcacctg accgcgagta atcacatcaa agcctccttt
    11701 gccaaaatgc tggatcactt tgttgggctc atctttacct gccagatatt cagacaaacc
    11761 atgttcatta ctcacgttga acaggttatg cgaataaccg cggcgtaagt cggcgtcaat
    11821 aaataacact ttttgatcgg actgggcgat caccgacgcc agagttgaac tgacgaacgt
    11881 tttaccactg tctggcgtcg caccggtgat catcagaatg ttattctccg tctccatcat
    11941 agcgaaatgc agactggttc gtagtgctcg tacggcttcc acagcagaat ccgccgggtt
    12001 atccaccgcc aggaagggga tatttttagt acgatggcgc tgctgattag aaaataaatt
    12061 tttcttacgc agacgggtgc gtttatccag ccactcggac atcgggatag tggcataaac
    12121 gctgatgccg tgctcttcca gttgttccgg tgcttctaca ccacgacgca acatcgcacg
    12181 cgccagcacg gcacccaccg aaataaacag gccaagaata aaaccaagca ccacattcaa
    12241 cgcttttttc ggtttcactg gctgcggctg agtgactgcc gggtcgataa tccgcacgtt
    12301 accaatggca ctggatttcg aaatactcaa ctcctgctgg cggttaagta attgcagata
    12361 taccgcacgg cccgcttcta cgtcacggct taaacgtagc acttcctgtt gagtggaagg
    12421 cattgccgat acccgcttat tcaggcgttt gcgttcttgc tccagcgtct ggcgtttttc
    12481 cagcagcgca cgataagttg ggtgatcttt cttatacagc tgggagatct ctgcctcgcg
    12541 gaaggtcagc tcattgagtt gattatcaac gttaacaatc tgctcaagaa cggctttggc
    12601 ttccagattc aagtctaccg aatcacgttg ttgacgatag gcattcagtt tttcctcagc
    12661 ctgatccaac tcactgcgta cctgcggtaa ctggcgctgc aggaactcaa gactttgcga
    12721 atcctgtgcc gcctgacgcg cgatattctg ctgcaaatag ttgttagcga tgctgttcag
    12781 tatatgagta atcagttgcg gatcgtcacc ggtcatggtg agttccagca tcccgctctc
    12841 tttactgcgc tcgctaacgg taaaggtttc ctgcagggcg ttaatcgctt ccagttcggt
    12901 gcgctggctc aggacaaact gtgttcctgc tttggcctta atgtccgcga tagtcagcgc
    12961 aacgccatct ttttccagac gctggccgac cataccattg acggtgaact cttcaccttc
    13021 cagcgtatag tggccgtttt ccccaaccgt cagcgtcagt tgctgatcct gaccattcag
    13081 ttgtggtata tgcatccagc tgatcgccag ctcacctggt ttctctttgg ttaatctcgc
    13141 ccagccgcga cccacaatcg gaaaatactt ctgctcaacg atgtcgcgca gattcagttc
    13201 agcaatggtt ttaccgagaa tcatgcgcga ttgcagcagt tggatctccg gtgcagactc
    13261 gggagatgag ttagggatca tatcgctcag gccgctgaga atggcgttgc cctgtttctg
    13321 ctcaacctgg accagagtat ctgcctgata aattggcgtg cttaacagcg agtaagcgac
    13381 agcgatcagc gtgaataacg cggtcacgct gataataaac ttacggtgat cccataactc
    13441 gccgaccaga cgaagcagat cgatctcatt ttcctgagtg ctgcctggtg gcgtattcat
    13501 atttttagtt gtcatcgtat tttataaact caaattaccg gctgaggcgc ttcgcccatt
    13561 cctgactggc gcgctccaac ataccgtaga catgttcaaa tgcgtcctga cttttacgat
    13621 agggatccgg gatctctttc tgttccagcc attgcccaaa cagcattgtc ttcccgcgga
    13681 cctcgggagc gattgcggta acctgagcaa tatgctctga ctccattgcc agaatcagat
    13741 cgtaatttcg tgccatttca gcagtgagct tacgtccggc atgtccttca agagatacgc
    13801 catgattagc agcaacatct gccgctgtcg catctgcagg gtgttttacc agaccatgaa
    13861 cacctgctga tttgactttt acgcctggca gacgtttacg cagcaagcgt tcgccaatgg
    13921 gcgaacggca aatgttgccg gtacaaacca ccaggattga gttaaatttt agttgggcca
    13981 tgtttgaatc caccgtgaag tctctgtcag gtcatgcacc ccggtaatcg tcggtaccag
    14041 cagagaaatc acacggttcc aacgagccag cggcgcagta gtgacataaa caatgtcgta
    14101 aggttcaagc tggaattccg ttccgagtat cattgccgat gcgtccttcg cattcagctg
    14161 ataaatattg gcgattttgc cattctgctt gttttgcgtt gcacgaataa caaaaatccc
    14221 cgtcgcgtcg gcgacatcct gattcatacc ctcagcgtta cccagcgctt ccgccagggt
    14281 cataccgctg cggtccattt tcagcgtgct ctgtttaccc acttcaccca tcacgaagac
    14341 tttcagatca tcgttgcgcg gaataaataa aatatcgccc gggtgcagca gtttgttctg
    14401 gcgcaagtcg ccacgctgca tcagcgcata gaggttcact ttggtcttta cgccattctg
    14461 agtgagcacc acgttgcgcc agtctgcatc tgcggtcaga cctcccgcgg cgttaatcgc
    14521 atccatgatg gtcagaggaa tattggtaat aggctgctga cccgatttgc tgacttcgcc
    14581 ggtgacgtac gctttttgcg agcggaacgc cgcgacgctg acatcgactt gcgggctttc
    14641 aatgactgaa tccagacgcg ctgtaatctc gttgcgtacc tgggtaagtg ttttcccggc
    14701 cactttcagg cgaccaatat aggggtagaa aatcgcacca tcggcgttaa cccagttgcc
    14761 ggtatcactg gcgctacggt attgccctgc gggggtggtt agttccgggt gatcccagac
    14821 cgtaaccatc aatacatccc caatgccgat gcgatattca tagttcgcga tttgctgctc
    14881 cagctccgga ttagcctgcg acataatcgg tttggcgcgc agctgatcaa tcaatcctgg
    14941 cgtgacggga tagatattga ccattttgtc taagtcatac tgattgtcag gtagttcaat
    15001 gacatccttg ttactggtcg ataaattctg accaggaaca agggtacatg ctgtcaaaga
    15061 cagaacagcc aatgtcagta ccgaaaactt aaaaatgttc tttttcattg gtttatattt
    15121 atcagtcatt gtaatgttat gaattacttc aggttattgt ccaaccggta cttctcgctc
    15181 tgccgtcatt ggatagagct ggtatttgcg tgacagcatt tgtccaccat cacgcgtcaa
    15241 tggtgtccac gaaaccaccg cgcggttgcg gttaggtcca atggtcatca agtcgaacgg
    15301 aatcgagata taaaaccctt tactaaagcc gccttcgccg tagtcatctt tagatacgtt
    15361 actgattgcc gcccataccc ctaccgccac gccgctgtca aagcgcttgg cgacgtcgat
    15421 cgttgcgcct ttatcttttg ccagatattg cccaacgcta agtttcatca gtacgccatt
    15481 gagcgtcggc gggttccagt aagccgtcac gaaacctgtg ggtgtggaat aatcggtgaa
    15541 gcgcatcatg ttgtcccagt cacgttgctt cacgtagtta acgtccacac ccagcgccca
    15601 gctggcatct agcgggcgat aaagcagctc ggaaccgaca ccggcgtaca tcgtttccag
    15661 ataaccgcca tacacctggc catagaaacc attgcctaag tcggcaaagt agttcgcctg
    15721 caagttgttg agataaacgt cattgcgaac gtaatcacgg atatgcgtgc gcacgcgggg
    15781 cagggtagag tccgcgggta atagtgaaga cttaaacttg tcgtagttat tgtaaatatt
    15841 ggtgaaaata ccgccatcaa gcagcaggtg gtcggtaaac cagtagcggg cgctggacat
    15901 cagccccagc tggaacatat agaaatcttc cgggccgccc agcgactgtg aaagtgttgg
    15961 gttgaaagag tagctaaagc gatcttcacg aatacgatag ccccgaccaa aggcagaaag
    16021 atcttcagct tcaacacgtt gttgttgcag tggctctgat tgaccaggcg ctgtaccggc
    16081 caactgtttg cgcaggctgg ctacgtcggt ttcggtagtc accatcgcca tatgctcgcg
    16141 cttttgcgtc acgctaatct tctcaacgcc ttgcggcagg ttattcacca gaatccggtt
    16201 ggcacgatcg accgcttcgc gagagtcacg gtatttgtat tgctgaccag acatatacag
    16261 cgtcttatcg cgtagctgaa tttccggtgc ttcaaaacct gcgttgtact tcagtgcggt
    16321 aagttggttt gctaccgtgg tgtactgcaa tccttcagat tccggcgcag gttgatatgc
    16381 cggttttggc gtatcgcgca gggcagggcg cagatcgttg aaattggttc gtaacgtgaa
    16441 gccaaacatc aacgtgttac cacgctcgta gctaaggtta agatctgccc agttggcagc
    16501 acgatacaca gcaccaacat tgaaatgact tgcctgcggc aatttgcccg caaaatcatt
    16561 ctggtaattg ttgccgtcgt attcgagttt cagacgcaga ggattccacg gcgtttgata
    16621 ctcaataccg ccaaaaatag aggctggacc acgaaagata tcgctaaagc tgatatctcc
    16681 cgcgtcgtga gactctgcgc gatggcaata tttatcgctt acccgacaaa acgggttggt
    16741 aatattgccc gcattaccgg cgtagcccca ggccatcccg agggtgaaat cgaacggtcc
    16801 ggcttgtttg ctggcgacca gatattcacc gtcgaacaga cccgtaccag caatatcacg
    16861 tttaccaaac gccacttgcg gtagccagta accttcttcc cacaggcgaa gtttaaaatc
    16921 gaatgatttg tctttgtatg actgatcgcc gctgaaatct tcccactggc tatatttgcg
    16981 tgtgcgcaca tccgtataac gaatggtgcc ttccagccat gggaacagcg ccacggacgt
    17041 ggagtagaaa cggtactgat cgttatcccg gtagttgacg ctgaattcac cttccggtgc
    17101 gatgcgcgca tttggcatct gcaacaatcc tgtgccgcca aagtctgatt gcgacgaacc
    17161 cagcggatcc gggtaggtta atacttcagc atgacaggca ctggagacgg caatggccag
    17221 gcagcttaaa agataagagt tcttcttcat cagtcaggaa cacgttgcgt taaaacagac
    17281 acaatctggt catttaaatc agcatatttt tcgggcagta catgcgcaga aaagcctaac
    17341 caaagctggc tgccaggcgg tggttccaca tgtcttttat tccacagggc aacaggggcg
    17401 acgacagcct caccctcagg cgtaatcacc ataacgttat tcttgtccgc gcctgcaaga
    17461 cgggtgtgat cttgcagata atcggtaacg ctacggcctg ccagccaggg caattgccca
    17521 gccccagaaa ccgcacccag cagagtaata gtcaccggac gctgtacggt atagagcgta
    17581 tagtccccga ccagcggggg attgctgttc tcatccactc gcacaaaatc agggtccagt
    17641 ttgaccggta agcgaccagt gatattgaga ttgagtaact gctggcgcac ggatttaatc
    17701 gttgccgcca catcgtcgtc agcttctgct tcccaggaag caagctgtgc catgacatgt
    17761 tggtagtctt taagcgcttt agctttcgct gcggagtcgg tcagcaaggc accaggccac
    17821 cagaggcgat cgcgcagttg cggttgtgtc accaactggg caacattctc tacggggcca
    17881 acggaaagtg tctgttgttc gccaggcaga taaatagtca ccattccttg cgcaaaggca
    17941 tggggtgtca ttacgtaaag gacgctggca atgaaatacg actgtaattt attcatggtg
    18001 cgggtttaag aaacgtcatt tctacgggaa taacccctgc gcctaacatt tgccgacttt
    18061 gacgaacctg gccagtagcc gaatcgaccc agaatgtgtt ttgccactgt ttttctggta
    18121 gcgtggaagt cacctcttcc tgccagacat tacacagcac atcgcgaccc gcaatattca
    18181 gcgtctctaa tccagcaaag gaaaaagtgg aactcagggt cgcagagcga aaatggttgt
    18241 cttcgctcca gagaatatcg cgcgtccagc ggctgccgtc ttttattgcc agcgcgttac
    18301 gcagagggtc ctgcccggaa tttgtcactt ccagcagatt attattaagt ttgacggttt
    18361 ttaaaagacg tccattgtgg gtaaccagca tggcgttatc ctgggataac catttgcttt
    18421 gttcttgttc ttgttctata tatcccagta ccacaaagat tcgttgccct tcattaaggc
    18481 gtaaatacat cgtggaataa ggcaacgcct ggatctgctg atccgctacg gtgatatcct
    18541 gattatcgaa taggctggcg cgaaatgtat cgaccatact ttgctggctg tgcgtacatc
    18601 ccgcaagaaa tagtgcgaaa atcgataaaa taagagggcg cacctttaat ccttgaaagg
    18661 ttaattaaaa aaatgcaaat ctctaaagcc cggtacgaat atcgcaaccg ggtatcattt
    18721 aactttaatt tcgacgcgtt aaagtggtat tagcgcgtag tggacgtggt ggttgaggtg
    18781 ttatgagaac cgccatcgcc actgtcattt gctgcaacaa ctgccaacgt cgccacacca
    18841 acagccgttg ccacaccgac agccgtcgcc gtaccgacag cgccagcagc cacgccacca
    18901 gtggtagctg cagtcgtggt tgcaccggta gtggtcgttg tggtgccagt tgtacccgtc
    18961 gttgcctcgg tcccgtttgc tgcttcaggg gcagcaaatg cagctggcgt cgtcaacatg
    19021 aaggccataa ggattgcaga aagcttgtgt ttcataactt ttcctttatt catcgcatgg
    19081 acaatacgta cgcttgagat caggattatc tactttgagt ataacatcct tgtgaaatct
    19141 ccctttcctc ataccgcttg cactatatac tgcgggtagg aaaggcgcgc agagggaaat
    19201 ataagattgt taacgatttc cctctggaaa tacgacttgt gagcataaga taaatcttag
    19261 aaaaatacaa attaaagata agaaaattgt tgagaaagct attaactatt gttggtaata
    19321 tagtttcaaa tggtactata actggctgtt aagtggcgtt tacaactatt aggtacagag
    19381 atattcggtt aaatggatta gtttcttgat gaaaaagtaa cttacgttat aatgtgtggt
    19441 gttgactatt gatctattcc aataaaacca gtcagtttta aacaagcagc gtcatattaa
    19501 gagagataaa catttgccgc tgttggtcca cgcaggccat ttacgcggca aaattccacg
    19561 cgtaatcctg gtataagcac ttctgcgtcg cggggagtga atgcggaaat atggacctgg
    19621 acttctttac gaccgtcgga ggggataatg aatcctttgc cgcttttgcg atcaaaggtt
    19681 ttgacaattc ctgtcatttt acgggacaaa caaattcctt actgaaaata ctgcgctgca
    19741 ctatacgggg ttaataaaat aaagccagcg atatttaaga ccgccggacg gctaaaataa
    19801 aatttgctta atctcaatta tcatgcgtta atagctgcgt cggtttgaaa gacagacagc
    19861 atacaaagta gtttactaaa gcagttctca ttatcaggca tcatcccctt cttttgagtc
    19921 tctctcctga acactaagta gtttctgtat taaagccctg tttgccgaaa ggcccaaaat
    19981 gaaggaagta aaatatgtct aataaaatga ctggtttagt aaaatggttt aacgcagata
    20041 aaggttttgg ctttatcact cctgatgatg gcagcaaaga cgttttcgtc catttcactg
    20101 ccatccagag caatgaattc cgcacgctga acgaaaatca gaaagttgaa ttttctattg
    20161 agcaggggca acgtggtccc gcggcagcga acgttgttac gctctaaggt tgccgttatt
    20221 actcaacatc tccatttccg ctgtccatgt tgccatggtt cacagtaccg cacatcggca
    20281 ttcgatgtga cggagcgaaa ccctttgggt gcaaagtgca ttttttgtaa atcgacgatg
    20341 atcacctttg ataacgtcgc gctgcaaata cgcactgacc atgcgccgct ggatttcaca
    20401 aaataatatc aggctccctc gtggagcctt ttttatatct gccttatttt tcttcaacgc
    20461 tgtatgtata gtaagcgata acctgttgat tattgaatct ttcggggaga tggcttataa
    20521 catttcttac ctgaccaggg taccgggaac caacacctta ctggcgtgtt gctgtctttt
    20581 aagaccagaa gaggttaaca gtgaatattg aagagttaaa aaaacaagcc gaaacggaaa
    20641 tcgccgactt tatcgcgcaa aaaatcgccg agctgaacaa gaatacaggg aaagaagtct
    20701 ctgaaattcg cttcaccgca cgagaaaaaa tgaccgggct tgaaagttat gatgtcaaaa
    20761 tcaaaataat gtgattttgt gaacatcacc ccgtgcgagg tgatgttccg cttgttgcta
    20821 atttagtgac caatcattgg cgattgcgga attaagcgtt ggtacaattc ctccggcacc
    20881 gggctttgcc atactcccgc atacattgcg taaccaatcg ccgcaaacat aatccccata
    20941 accagtagcg tcattaacca gccagacaac gcaaaggctt ttttatttgc cgcaggtttt
    21001 tgcagtgaaa aggtcaatgt tgaggctacc ggacatgact ctacgcaagt catacagccg
    21061 gtacattcca ctgttcgtac ctgaattaat ttatcgaccg ggatccgtga tgggcaactt
    21121 tttgcgcatt tgccacaatc gatacaactt tcggcattgc gacgaatctt aaacggcgat
    21181 aatagcgaaa ccacgcccat cagcgcgcca tatgggcaaa gataacgaca ccaggcatgg
    21241 cgaataaaca ggctggcaat cagcaaaacg atcatgctga ttaatgtcgc ggtccccata
    21301 tgacgaaaga aatcgagcat tttaacgtcc atcaccacgc tgtagggcga caacataaaa
    21361 tagtgaatcg cctgagcggg catcaataac gcgatataga gaaaaaaact caacagcaaa
    21421 tatttcacgc cgcgcagagg aatatccagc cagcggggaa ggacaaattg ccgaccaaac
    21481 agtttgttac cgagatcgcc gattaattca gaaagcgtac caaccgggca taaccatgaa
    21541 caaaaggcct ttttgagtaa tagactgatg acgataaaag agaccaataa cagcatcgcg
    21601 gcggcgtgga cggacggtaa ctgacctgtt gcaaggctat atttcagatt catcagaccg
    21661 gcaatcggta gccagccttc gatacctccc ggtctggcga caaatgtcgt actacctgcc
    21721 gtttcgtaat agcgcaccca ataccagaac gtgatggcaa tataaatatt aattgccaac
    21781 agtaataatt gcgtcgcttt acgccaggtc gtggcattgc gccagtcatt ccacggtaat
    21841 ttgccgcccg tcgtgcctgg ccgccgctgc cagcgggttc tttttttctc tgccatgatt
    21901 ttgccagtcc gttaagttgc ataccaaatg ccactattct agttgttctt aactggctga
    21961 tattgattca aatcgcgttc aggtctttct tattaaccat gcttccagag cggcaacact
    22021 gcgtgtaatt tcttcgtgtg gaaggggggc agataatggc tgctgctcca gttgtgcgca
    22081 ttgctggctg gcgctacgca ttcccagact cgagcaactg ctttttagct gatgtgcggc
    22141 acgctttatt ttctcgttat cctgactggc gcgggcaatg tcgatttcat cgagaagcgg
    22201 cagggcatgt tgtttaaata atatcagcca ttcgtggatc ttctccgccc ccattaaatg
    22261 agcatcttca ttgagttgcg atacatccag tggttgatcg ttattgactt gcagttggag
    22321 atagtgcgcc agtaattgac cgagcacttc acgcggcacc ggtttaggga taatcccgcg
    22381 aaatagcgaa ctggtacgct gtcgcagtgt ttcgtcaatg acatgggcgc taaagccaat
    22441 caaaaccagt gacggatatt gctgtgccag ttgccgggca agggtaatgc cgtcgatatc
    22501 cggcagatca aaatccacca gtgcggcagc aaacggttcg ctattttgca atgcctctat
    22561 tgcctgcgcg gcatttccta cggcaacgac ctgcgcacca ctggtgttca gcatttcgac
    22621 agtaattcgc tgggtgagcg ggttatcttc aattaacagc aaacgcaaat cgtccagacg
    22681 taccgcctga ttgactgttt tgggcacggg tgcagtggca atacgtaacg gcaagcgtaa
    22741 acaaaaacag cttccaacct ccggcgtgct ggtggcgctc agttcgccac ccatcgcctg
    22801 ggccaggcgg ctactgatag tcagccccag cccggtgccg ccgcgtttgc cgctaacctg
    22861 aacaaatggc tggaagattt cagccagttt cgcgggatca ataccgcagc cgctgtcttc
    22921 cacttcgacc agccattgtt cgccatcagt gcgactacgc aggataatgt gcccttcgtc
    22981 ggtgaaacgc agggcgttgc tcaacaggtt ggtgatgacc tgacgaatac gccgcggatc
    23041 gcccattaac gcagtcggta catcgtcggc aatttctgtt gccaggcgaa tcgggcgacc
    23101 tttcaccctt ccgctcatta attgcagggt actttccagc agcgggcgcg gttcaaaggg
    23161 ttcatcactg accgaaacat tcttgccgcc tgcttcgata gccgaataat cgagaatatc
    23221 gttgaggatg gtcagcaacg attcaccaga gtcggtaatc gcccgcaaat catcgcgctg
    23281 ggcgttaagt gcggggttat ctgccagcag ctgtgcggtg ccgagaatac cgtacagcgg
    23341 tgtgcggatc tcatggctca tcgctgccag aaacgccgat tttgcctggc tggctttttc
    23401 tgcttccgcc cgtgcctgtc ggtgttctat caccagttcc tgcaattcag ctgtacgcgc
    23461 tttgacctgc gccgccagct gttcgcggtg gcgattcagt gcatgaacac tgctgcgaaa
    23521 cgcatccatc agccgcccga tggtgtccag ttcccgcacg ccagcggttt ccgggaaagg
    23581 ggagtcgata tcaccatcca gcaagcgttg cagcgcctgc gtttgttctg caagagggcg
    23641 cgtgactgag cgataaacca cgcgccagag gatcagaatc agtgcgcaaa gagaaactaa
    23701 tcccagcaat aacaggctgt attgcccgcg tgcactggct ttttccagat gtgccagccc
    23761 gtgctgatta cgcagctcaa tggtgtcgac cagctgactg acttcgctac taaactgcgc
    23821 gaattgggcg atgttatttt gggcgagagt tttcaggtga ttgctgattt cactgtcctg
    23881 ctgatacagc gccagcaaat cgctatattg gctaacggta gttaacgttg ttgctacctg
    23941 agtacgaaca cccggatctt caatgcgtat ttgccgacgt tgcagaattt tcaccgcatt
    24001 attgagctgc ttttccagcg ttggcgcatt tttctggatc tgttccagcc ccagattcat
    24061 caccatttgc tgcacccgca gggcgctaag gcgcagttca ttcatctggt taacatactc
    24121 aagatcgata tcaatcagcc gatcgagtgc actttctgcg gcctgacgct gatgttgttc
    24181 gatcaaatcg taaatcccgg cctgggtcgc tccggcggag gttgccgcat tattcgcctg
    24241 accttgcgcc aggcgtgcga tctcatcggc ggcggcgact atccgctgac tgagttgctg
    24301 ttgttgctgg cgcagttgca aacgctgtcc caccagttcc ccttgctgac gtaacgaacg
    24361 ggagatctcc tgctcctgtt gttcaatagc ggtggtgtca aaaccttgtt cccgtaacgc
    24421 ttgcagcaac gcattaatct tcaggctttg tgcggtgagc attcgcccct gcgcctgcca
    24481 catcttttcg ttatcggcac tggtcaggtt ctgcgcggcg aaaagttccc aggcgctggc
    24541 ttcgctcaac tggcgcgcca tattcatggt aggaatcaat gcctgagtgt tgtctttttc
    24601 cacctggctg ataaagcgca ggttgtacca tcccaccagg gtactggtca gggttaacag
    24661 cgccatcagg gcaaagccca tccagagtct tcgggtcagg gttaaattca cggtcggtgc
    24721 actttgggtg aaaaaggttg agtcgcaaag cggaatgcat ctagcataaa gccttattat
    24781 tgatgaggct atcatgcgcg tactgctatt gttactactt tcccttttca tgttatcggc
    24841 attttcggct gataacctgt tgcgctggca tgatgcgcag cattacacgg tgcaagcctc
    24901 tacgccgctt aaagccaaac gcgcatggaa actgtgcgcg ctttatccca gcctgaaaga
    24961 ttcatactgg ttatcgttga actatggtat gcaggaggct gctcgccgct acggtgtgga
    25021 tttaaaagtg ctggaggcag gcggctacag ccagttggct acgcagaaag cacaaatcga
    25081 ccagtgtaaa cagtggggcg cagaggccat tttgctcggt agtagcacga cgtcatttcc
    25141 cgatctgcaa aagcaggtag caagtctgcc ggtgattgaa ctggtgaatg ctattgatgc
    25201 tccccaggtg aaaagccgcg ttggtgtgcc ctggtttcag atgggctatc aaccagggcg
    25261 atatctggtg caatgggcgc acggtaaccc actgaacgtg ctgttgatgc ccggccccga
    25321 taacgccggg ggcagtaagg agatggtaga ggggtttcgc gcagccattg ccggaagccc
    25381 ggtacgtatt gttgatattg cgctcggtga taacgatatt gaaatccagc gtaacctgtt
    25441 gcaggagatg ctggaacgcc atccagaaat cgacgtcgtt gccggaacgg ccattgcggc
    25501 agaggcggca atgggggaag ggcgcaacct gaaaacgccg cttaccgtgg tgtcgtttta
    25561 tctttcacat caggtgtatc gtgggttgaa gcggggaaga gtgattatgg cagccagcga
    25621 tcaaatggtc tggcaggggg aactggcggt tgagcaggct atcaggcaat tacaggggca
    25681 atcggtgtct gataatgtca gcccaccgat tttagtcatg acgccgaaaa atgccgaccg
    25741 cgaacatatc cgccgctcgc tgtcaccagg gggatttcgt ccggtttatt tttatcagca
    25801 cacatcagcg gctaagaaat aaccttcgcc atgttgtgtc accagtaaat ccgcactgag
    25861 tttatggcgt aaacgacgaa ttaacacatc gacggtgcgc aggtcagggt tttccacccg
    25921 acgcgcagag agcatacgca gcagacgttc acggctgaga atttcgcccg gattagtcac
    25981 aaatgccacc agcatttcat actctgcgcg ggtcagttta atcggctcgc catcccgctc
    26041 cagcgtatgg cgcgacacat tcaggcaata accggcaaag cgatagcagt tgtcctgagt
    26101 gtgcggttgc gcctgtcgcg cgaggtcgat tcgccagagc aagtttttca ctcgtaccac
    26161 cagttcgcgc agttccagcg gtttggtgac gtaatcgtct gcgcccattt ccagcccaac
    26221 aatacggtca atccgatcgc tgcgtccggt aaccagaata atccccaccg ttgagcgttc
    26281 tcgcagggcg cgggttaaca tcaggccatt ttcatcgggc aaattgatat ccagcagaat
    26341 caaatccacc ggctgattct gcataatttc ccgcagcccg gcaccgctcg ccgtaacgga
    26401 aacggtatac ccctcctgag tgaagtaaga ttgtaatcgc gcctgggtaa ccggctcatc
    26461 ttcaacaata acaatgtgat gtggcatcag agggttttac tcattctgtt catatctgtt
    26521 catattctgc cgtaagctgt tcatcctgac cagtgccgct gttcatattt gctcattaag
    26581 atcgcttcac taaaccataa ttttacaggg gctattatgc ggaaactctg gaacgcgcta
    26641 cgccgaccca gtgctcgttg gtcggtactg gcgctggtcg ctactgggat tgtgattggc
    26701 attgcgctga ttgtattgcc acacgttggg atcaaagtca ccagcacaac cgaattttgt
    26761 gtcagttgcc acagtatgca accggtgtat gaagaatata aacagtcggt gcatttccag
    26821 aacgcctccg gcgtgcgagc tgaatgccat gactgtcata tcccgccgga tattccaggc
    26881 atggtgaagc gcaaactgga agcgagcaac gacatttacc agacctttat tgcccactcc
    26941 attgatacac ctgagaaatt tgaagccaaa cgcgcggaac ttgccgagcg tgaatgggcg
    27001 cgaatgaaag aaaacaactc ggcaacctgc cgctcctgcc ataactacga tgcgatggat
    27061 catgcgaagc agcatcctga agcggcgcgt cagatgaagg tggcagcgaa agataatcaa
    27121 tcctgcatcg actgtcataa aggtattgcc caccagttac cggatatgag tagtggcttc
    27181 cgtaagcagt tcgatgagct gcgcgccagt gctaatgata gtggtgacac gctgtactcc
    27241 attgatatta agccgattta tgcggcgaaa ggcgataaag aagcgtctgg ttctctgctg
    27301 cctgcttcgg aagtgaaagt ccttaaacgt gacggcgact ggctgcaaat tgaaatcact
    27361 ggctggacgg aaagtgcagg acgtcagcgt gtactcaccc aattcccagg taaacgcatc
    27421 tttgttgcct cgattcgtgg tgatgtgcag cagcaggtga aaacactgga gaaaaccacc
    27481 gttgccgaca ccaataccga gtggagcaag ttgcaggcca ctgcgtggat gaagaaaggc
    27541 gacatggtga acgatatcaa accgatctgg gcttatgcgg attcgctgta caacggcacc
    27601 tgtaaccagt gccacggcgc accggaaata tcccactttg acgctaacgg ttggatcggc
    27661 acgctcaacg gcatgattgg ctttaccagc ctcgataaac gtgaagaacg caccttgttg
    27721 aaatatctgc aaatgaatgc gtctgacacc gcaggtaaag ctcacggcga taagaaggaa
    27781 gaaaaataat gaacaataac gatctctttc aggcatcacg tcggcgtttt ctggcacaac
    27841 tcggcggctt aaccgtcgcc gggatgctgg ggccgtcatt gttaacgccg cgccgtgcga
    27901 ctgcggcgca agcggcgact gaggctgtca tctcgaaaga gggcattctt accgggtcgc
    27961 actggggagc tatccgcgcg acggtgaagg atggtcgctt tgtggcagca aaaccgttcg
    28021 aactggataa atatccgtcg aaaatgatcg ccggattgcc ggatcatgta cacaacgcgg
    28081 cgcgtattcg ttacccgatg gtacgcgtgg actggctgcg taagcgccat ctgagcgaca
    28141 cctcccagcg cggtgataac cgttttgtgc gcgtgagctg ggatgaagcc ctcgacatgt
    28201 tctatgaaga actggaacgc gtacagaaaa ctcacgggcc gagtgccttg ctgaccgcca
    28261 gtggttggca atcgacgggg atgttccata acgcttcggg gatgctggcg aaagctattg
    28321 ccttgcatgg taatagcgtt ggtacgggcg gagattactc taccggtgct gcgcaggtga
    28381 tcttgccgcg cgtggttggc tcaatggaag tgtatgaaca gcaaacctcc tggccgctgg
    28441 tattgcagaa cagcaaaacc attgtgctgt ggggctccga tttgctgaaa aaccagcagg
    28501 cgaactggtg gtgcccggat cacgatgttt atgaatatta cgagcagttg aaagcgaaag
    28561 tcgccgccgg tgaaattgag gtcatcagca tcgatccggt tgttacatcc acccatgagt
    28621 atctggggcg cgagcatgtg aagcacattg cggttaaccc gcaaactgac gtgccgctgc
    28681 aactggcgct ggcgtatacg ctgtacagtg aaaacctgta cgacaaaaac ttcctcgcta
    28741 actactgtgt aggttttgag cagttcctgc catatctgct gggtgagaaa gacggtcagc
    28801 cgaaagatgc cgcatgggca gaaaaactga ccggcattga tgccgaaacc attcgtgggc
    28861 tggcacggca gatggcggcg aacagaacgc agattattgc tggctggtgc gtacagcgta
    28921 tgcagcacgg tgaacagtgg gcgtggatga ttgtcgttct ggcggcgatg ctggggcaaa
    28981 ttggcctgcc aggtggtggt tttggttttg gctggcacta taacggcgca ggcacgccgg
    29041 ggcgtaaagg cgttattttg agtggtttct ccggctctac gtcgattccg cctgttcacg
    29101 acaacagtga ctacaaaggc tacagcagca ccattccgat tgcccgtttt atcgatgcga
    29161 tcctcgaacc ggggaaagtg atcaactgga acggtaaatc ggtaaaactg ccgccgctga
    29221 aaatgtgtat ttttgccgga actaacccgt tccatcgcca tcagcagatc aaccgcatta
    29281 ttgaaggctg gcgcaagctg gaaacggtta tcgccataga taaccagtgg acctcaacct
    29341 gccgctttgc cgatatcgtg ctgcctgcga ccacgcagtt tgagcgtaac gatctcgacc
    29401 agtacggcaa ccactccaat cgtggcatca tcgccatgaa acaggtcgtg ccgccgcagt
    29461 tcgaggcgcg caacgacttt gatattttcc gcgagctgtg ccgccgcttt aatcgcgaag
    29521 aagcctttac cgaagggctg gacgagatgg gctggctgaa acgcatctgg caggaaggtg
    29581 tacaacaagg caaaggacgc ggcgttcatc tgccagcgtt tgatgacttc tggaataaca
    29641 aagagtacgt cgagtttgac catccgcaga tgtttgttcg ccaccaggca ttccgcgaag
    29701 atccggatct cgaaccgctg ggcacgccga gtggcctgat tgagatctac tcgaaaacca
    29761 tcgccgacat gaactacgac gattgtcagg ggcatccgat gtggtttgag aaaatcgaac
    29821 gctcccacgg tgggcctggc tcgcagaagt atccgttgca tctgcaatct gtgcatccgg
    29881 atttccgact tcactcgcag ttatgtgagt cggaaactct gcgtcagcaa tataccgtag
    29941 cgggtaaaga gccagtattt attagcccgc aggatgccag cgcgcgcggt attcgtcacg
    30001 gtgatgtggt acgcgtcttt aacgttcgtg gtcaggtgtt ggctggagct gtggtttctg
    30061 accgttatgc acccggcgtg gcgcgaattc acgaaggggc atggtacgat ccggacaaag
    30121 gcggcgagcc aggggcgctg tgcaaatacg gtaaccccaa cgtgttgacc atcgacatcg
    30181 gtacttcgca gctggcgcag gcgaccagtg cgcacactac gctggtggaa attgagaagt
    30241 acaacggaac agtggagcag gtgacggcgt ttaacggccc cgtggagatg gtggcgcagt
    30301 gcgaatatgt tcccgcgtcg caggtgaaat catgaccatg ctgacagcac aacagattgc
    30361 ctgtgtttac gcctggctgg cgcagttgtt ctcccgtgaa ctggacgatg aacaactgac
    30421 gcaaatcgcc agtgcgcaga tggctgaatg gttttcgttg ctgaaaagcg aaccgccgct
    30481 cactgcggcg gtgaatgggc tggaaaacag tattgccact ctgacagtac gtgacgatgc
    30541 ccgtctggaa ctggccgctg acttttgcgg cctgtttctg atgaccgaca aacaagcggc
    30601 gctgccgtat gcatcggcct acaaacagga cgagcaagag attaaacgct tgttagttga
    30661 ggcggggatg gaaaccagcg gcaatttcaa cgaaccggca gatcatctgg cgatctatct
    30721 cgatttgttg agtcatctgc atttttcgct gggagagggg accgttcctg cgcgaagaat
    30781 cgacggtttg cggcaaaaaa cactgacggc tctgcgggaa tggttaccag agtttgctgc
    30841 gcgttgtcga cagtatgaca gctttggttt ttacgcggca ctaagccagt tgttgcaggt
    30901 gttggtggag tgcgacaacc aaaagggata atgccgattt gtaggcctga taagacgcgt
    30961 taagcgtcgc atcaggcatt atggcgcagt tgccggatgc ggcgagaacg ccttatccgg
    31021 cctacgggaa cagtgctttt tgtgggcctg ataagatgcg ttaggcgtcg catcaggcat
    31081 tagtgcgcag ttgccggatg cggcgagaac gccttatccg gcctacggga acagtgcttt
    31141 ttgtgggcct gataagatgc gttaagcgtc gcatcaggca ttatggcgca gttgccggat
    31201 gcggcatgaa cgccttatcc ggcctacggg aacagtgctc tttgtaggcc tgataagacg
    31261 cgttaagcgt cgcatcaggc aacaaactca cgggtgagcc acaaaccggg aaagccgctg
    31321 gcgcagcagg cggttttcct gcttcaggtg cgcaatatca tccattaacg tcagcgccac
    31381 cgcgatcccc ggccagtcca gagccagttc atgacgcagg cgtaccgcgc gttgcaccac
    31441 aatggcggca tggtcgtcaa atacccaggt tgtctcctgg atctcactcg gttcaaccac
    31501 ccccaaaccg acaatttcat tcaactcctc ttcagagatg ccggtatgca ggcaaaattc
    31561 ggtaatagta aaagtcaccg taacattagc cattatgctt tcccccaatc tttacgtggt
    31621 tcaaaagacg actgggcgtc tgccagttgc tgccacagcg cggcagtgtt ttcatccggt
    31681 ttcggcggca tcacgatttt cagtaccgca tacagatcgc cggtctgttt tttgctcacc
    31741 agacctttgc ctttaacgcg caatcgttgc ccggcctggc tgccaggcgg gatagtcagc
    31801 aaaatgcttt ctttcagtgt tggaacggtg actttagtac ccagcgccgc ttcccacggg
    31861 ctaaccggca ccactatttc cagatcctgg ccgacaatat caaacagcgg atgtggcgca
    31921 atgtgaatca ccagccacag gtcgccattt ggaccgccgt tttcgcccgg cgtcccctgg
    31981 cctttcaggc ggatgcgttg accattgccg acgcccgccg ggatcttcac attcagcgtt
    32041 ttcgggattt cctgttcgat catgccaaag gcgttataaa ccggcaggtt atagctgatg
    32101 gtacgcttat gctcagtaag cgtttcttcg aggaataccg ccacttcgat ttcaatatcg
    32161 tggccgcgtg tggcggggcg ttgacggctc tggcgggcat gctgaccgaa aattgacgag
    32221 aagatatcgt caaaatcttc ggcgttaaaa ctctgaccgt cgccatgatg gaactgacgg
    32281 ttaaattgcg gatcgttgcg atgttgccac atctgatcat actcagcgcg acgttgttca
    32341 tcacttaaca cttcccaggc ttcggcgacc tctttgaagc gggcttcggc atccggttct
    32401 ttgctgacat caggatggta tttgcgggcc agtcgacgat aggcggtctt gattgtcttg
    32461 agatcgtccg tcggtttcac gcccatgatg gcgtaataat cctttaattc catagcgtta
    32521 tctcgcgtaa atcaacacaa attgaaggaa cccctgtaag gtaactccta taagtgtagg
    32581 gtaatcctca aaatttcata tgccaacaca gaatatgtta ttgaaatcat cgcggagggg
    32641 aggtcgcctc aaggtgggag gaacatattt tatttacctc actcctgaac ttttggctca
    32701 tgcttacaag ttaaacaata aggaaaggaa tgcacaactc gactattta
//
LOCUS       DAZFJL010000041        32140 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFJL010000041 DAZFJL010000000
VERSION     DAZFJL010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.041"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(69..1829)
                     /gene="ycaO"
                     /locus_tag="ACHJ8C_001886"
     CDS             complement(69..1829)
                     /gene="ycaO"
                     /locus_tag="ACHJ8C_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO5551748.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTIYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWSFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1958..2650
                     /locus_tag="ACHJ8C_001887"
     CDS             1958..2650
                     /locus_tag="ACHJ8C_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO5551749.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGERQLCPRCANP
                     EWTKASRAKRVT"
     gene            2849..3937
                     /gene="serC"
                     /locus_tag="ACHJ8C_001888"
     CDS             2849..3937
                     /gene="serC"
                     /locus_tag="ACHJ8C_001888"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO5551750.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGFRAVKPMSEWQLSDNAAYMHYCPNETIDGIAID
                     ETPNFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVVAMDKINQQKAELL
                     YEVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4008..5291
                     /gene="aroA"
                     /locus_tag="ACHJ8C_001889"
     CDS             4008..5291
                     /gene="aroA"
                     /locus_tag="ACHJ8C_001889"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO5551751.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNVLTALGVSYTLSADRTRCEIIGNGGPLHAKSALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFNGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCMRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDFIRITPPEKLKFAEIATYNDHRMAMCFS
                     LVALSDTAVTILDPKCTAKTFPDYFEQLARISQSG"
     gene            5461..6225
                     /gene="ycaL"
                     /locus_tag="ACHJ8C_001890"
     CDS             5461..6225
                     /gene="ycaL"
                     /locus_tag="ACHJ8C_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO5551752.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGINTDLAISSGLNAYKAATL
                     SDADAKAIANQGCVEMDSGNKVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYITSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6398..7081
                     /gene="cmk"
                     /locus_tag="ACHJ8C_001891"
     CDS             6398..7081
                     /gene="cmk"
                     /locus_tag="ACHJ8C_001891"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO5551753.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7192..8865
                     /gene="rpsA"
                     /locus_tag="ACHJ8C_001892"
     CDS             7192..8865
                     /gene="rpsA"
                     /locus_tag="ACHJ8C_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO5551754.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9025..9309
                     /gene="ihfB"
                     /locus_tag="ACHJ8C_001893"
     CDS             9025..9309
                     /gene="ihfB"
                     /locus_tag="ACHJ8C_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO5551755.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9515..11779
                     /locus_tag="ACHJ8C_001894"
     CDS             9515..11779
                     /locus_tag="ACHJ8C_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO5551756.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTVTLAFLTLFACVLAFIPV
                     KTVRYIVLTLLFFVWGILAAKQILWAGETLTGATQDAIVEIIATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQVWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGCWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAIWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPFILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCLPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     IDDGTNSILLTGDIEAPAEQKMLSRYWQQMQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11816..13564
                     /gene="msbA"
                     /locus_tag="ACHJ8C_001895"
     CDS             11816..13564
                     /gene="msbA"
                     /locus_tag="ACHJ8C_001895"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO5551757.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLMWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13561..14547
                     /gene="lpxK"
                     /locus_tag="ACHJ8C_001896"
     CDS             13561..14547
                     /gene="lpxK"
                     /locus_tag="ACHJ8C_001896"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO5551758.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAIAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGH"
     gene            14584..15816
                     /gene="ycaQ"
                     /locus_tag="ACHJ8C_001897"
     CDS             14584..15816
                     /gene="ycaQ"
                     /locus_tag="ACHJ8C_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO5551759.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVVRSPYLVLFSRLGDYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHAAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTRRVMPEWNDERDLVSQAEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYIPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     SQGLFTDCRAGWEIDPVA"
     gene            15868..16050
                     /gene="ycaR"
                     /locus_tag="ACHJ8C_001898"
     CDS             15868..16050
                     /gene="ycaR"
                     /locus_tag="ACHJ8C_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO5551760.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16047..16793
                     /gene="kdsB"
                     /locus_tag="ACHJ8C_001899"
     CDS             16047..16793
                     /gene="kdsB"
                     /locus_tag="ACHJ8C_001899"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO5551761.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKDLESVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLKRVRAEMR"
     gene            16947..17840
                     /locus_tag="ACHJ8C_001900"
     CDS             16947..17840
                     /locus_tag="ACHJ8C_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO5551762.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSAPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17817..18596)
                     /gene="elyC"
                     /locus_tag="ACHJ8C_001901"
     CDS             complement(17817..18596)
                     /gene="elyC"
                     /locus_tag="ACHJ8C_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO5551763.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPIADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPRKQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            18732..19517
                     /gene="cmoM"
                     /locus_tag="ACHJ8C_001902"
     CDS             18732..19517
                     /gene="cmoM"
                     /locus_tag="ACHJ8C_001902"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO5551764.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19514..20836
                     /gene="mukF"
                     /locus_tag="ACHJ8C_001903"
     CDS             19514..20836
                     /gene="mukF"
                     /locus_tag="ACHJ8C_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO5551765.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMVLR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20817..21521
                     /gene="mukE"
                     /locus_tag="ACHJ8C_001904"
     CDS             20817..21521
                     /gene="mukE"
                     /locus_tag="ACHJ8C_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO5551766.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            21521..25981
                     /gene="mukB"
                     /locus_tag="ACHJ8C_001905"
     CDS             21521..25981
                     /gene="mukB"
                     /locus_tag="ACHJ8C_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO5551767.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEFLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAVQLNQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QSVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPYQAS"
     gene            26242..28089
                     /gene="ldtD"
                     /locus_tag="ACHJ8C_001906"
     CDS             26242..28089
                     /gene="ldtD"
                     /locus_tag="ACHJ8C_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO5551768.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDVVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMTRDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTIETAETKPMDKQTTSRSKPAPVVRAAYENELVEAVKRFQTWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28271..28819
                     /locus_tag="ACHJ8C_001907"
     CDS             28271..28819
                     /locus_tag="ACHJ8C_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO5551769.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28846..29493
                     /gene="gloC"
                     /locus_tag="ACHJ8C_001908"
     CDS             28846..29493
                     /gene="gloC"
                     /locus_tag="ACHJ8C_001908"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO5551770.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29543..30733)
                     /gene="aspC"
                     /locus_tag="ACHJ8C_001909"
     CDS             complement(29543..30733)
                     /gene="aspC"
                     /locus_tag="ACHJ8C_001909"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO5551771.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAALHKELIVASSYSKNFGLYNERVGACTLVAADSETVERAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(30918..32006)
                     /gene="ompF"
                     /locus_tag="ACHJ8C_001910"
     CDS             complement(30918..32006)
                     /gene="ompF"
                     /locus_tag="ACHJ8C_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO5551772.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEESSLGKGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7839 a         7808 c         8627 g         7866 t
ORIGIN      
        1 attaaaaaaa cgccccgtaa tataactcag acgaatcatt aagcctacat tgcgtaggct
       61 atttgatttt attttgccca gaatgctgct ttggcgcgtt gcagcttttc gtaggccttc
      121 aacaacgact gatgtgcagc aaacgcgtgc agatcgctat ctactggttg caagccataa
      181 aacgccgctt cgccgctcat tgccgcactg gcggcttcca cggcatctgc gccgtacatg
      241 cgaacaaacg cattcagata ttgcagcggc tggcgatctt cttcctgcgc cagtaataac
      301 aacgtttgca ggcagcgata ataattggcg cgttccgggc tgaatactga tgagttaaat
      361 tccatcgtcc attcggtcca gaccagagcc tgttccagat cgccaccagc cagcgccagc
      421 atggctttta attcaccgat acgcagagtg taccagccgt tatccgaccc ggtcgccaga
      481 cccaacagtt cacgcacgcg ggtaaagtca tcaaaacctt cttcatccag ttgctcgatg
      541 aggttcaggt aatcttcctt ttcccactcg ctacctggta gcgaaagaat cgtttcacgt
      601 aaatggctgc ccatactgtt attcgccagc cacagatctt cagccggata aatatcggac
      661 atgccaggca cgataatacg gcaagcgtag acacccagat gctcgtaatc ggcaatataa
      721 acttctttat cttccttttt gaagatagcc atcagcgtgg cgaactcttc ttccgtggtg
      781 ccggagaagc tccagtcaac aaacggataa tcggcatcct gcttgaacag gtcccaggag
      841 attaaaccgc tggaatcgat aaagtgcgtt tcgaggttgg tatgttcagc gacttcttca
      901 tcatcgaagg ttggtggagt aaacacatcc agatctttca ggccacgacc ttgcagcagc
      961 tcggtcacgg tacgttccag tgcaacgcca aaatcaggat gcgcaccgaa tgaggcaaag
     1021 caggtaccgt tagcaggatt gaacagtacc acgcaaatca ccggatactg gccgccaagc
     1081 gaaccgtcat aagcgaagat cgggaaacct tccgcttcca gcgtttcgat cgcttcaact
     1141 actgctggat aacgtgccag cacgtctgcc gggatttccg gcaagctgat gctttcagca
     1201 ataatgcggt ttttcacgta acgttcgaaa acttcggaca acccctgaac gcgtgcttcg
     1261 ttacgggtat tgccagcaga cataccgtta gaaacgtaca agttaccaat gatattcatc
     1321 ggaatataaa tggtctgatt gtcggactgg cgcgtaaacg gtagaccgca aataccacga
     1381 tcttcgttac cggattgcag gtcaatcagc atactaccgg tcagttcatt ctccggatcg
     1441 taaaatgcgc gcagacggtc atcgagcagc ccttctggta catcgtcatt ttcggtcagt
     1501 gggaaccatt tttcgttggg ataatgcacg aatgggccgt tggcgatggt ttcgcccagc
     1561 cagaagtccg caaaaaagta gttggttgag agacgctcga aatattcacc gagggcagaa
     1621 gccagcgccg cttttttggt tgcgccttta ccgttggtaa aacacagtgc gcactctttg
     1681 tcgcgaatat gtacagacca gacgttaggc accggattca gccaggaggc ctcttcaatc
     1741 tgaaaaccga ggtctgaaag tttttgctgg aagcgtgcga tggaatcttc cagagcggca
     1801 tctttgccgg ggataaatgt ttgcgtcatg aaaatcactt tagtcgtacg gaaagcgcgc
     1861 aataatacgg gttttatctc aaaggcgcta tcaccgccgc catgccgatg aactgttggc
     1921 tatgctttta gcggataaac cacgataaga gcataaaatg aaggcgttcg atctccaccg
     1981 tatggcattt gataaagtgc cttttgattt ccttggcgaa gttgcactac gtagtcttta
     2041 tacctttgta ctggtttttt tgttcctcaa aatgaccgga agacgcggtg tgcggcagat
     2101 gtcgctgttt gaagtgttaa tcattctgac actgggttca gcggcgggag atgtggcgtt
     2161 ttatgatgat gtgccgatgg tcccggtact tatcgtcttt attactctgg cgttgttata
     2221 ccgcctggta atgtggttga tggcgcacag tgaaaaactg gaagatcttc tggaaggcaa
     2281 gccggttgtc attattgaag atggcgagct ggcctggtcg aaactcaata actccaacat
     2341 gacggaattt gagttcttta tggagctacg attgcgcggt gtagagcagt tggggcaggt
     2401 acgtctggcg attctcgaaa ccaacgggca aatcagtgtt tatttctttg aagatgacaa
     2461 ggtgaaaccg ggcttactta ttttacccag tgactgtact cagcgttaca aagtggtgcc
     2521 ggagtcggcg gactatgcct gcatccgttg cagtgaaatc attcatatga acgcggggga
     2581 aagacaatta tgtccgcgct gtgcaaatcc agaatggacg aaggcaagtc gggctaaacg
     2641 ggtgacctga cagccaaaac atcggttttt tgcaaataat ccgagagatt cttttgtgtg
     2701 acgcgtgcct catttttgcc ctcaatggtt ttactcattg cgatgcgtgt cactgaatga
     2761 taaaaccgat agccacagga ataatgtatt gcctgtggtc gcaattgatt gaccgcgggt
     2821 taatggcaac gcaacgtggt gaggggaaat ggctcaaatc ttcaatttta gttctggtcc
     2881 ggcaatgcta ccggcagagg tgcttaaaca ggctcaacag gaactgcgcg actggaacgg
     2941 tcttggtacg tcggtgatgg aagtgagtca ccgtggcaaa gagttcattc aggttgcaga
     3001 ggaagccgag aaggattttc gcgatcttct taacgtcccc tccaactaca aagtattgtt
     3061 ctgccatggc ggtggtcgcg gtcaatttgc tgcagtaccg ttgaatattc ttggtgataa
     3121 aaccactgca gattatgttg atgcgggtta ctgggcagca agtgctatca aagaagcgaa
     3181 aaaatactgc acaccaaatg tgtttgatgc gaaagtgacc gttgatggtt ttcgcgcagt
     3241 taagccaatg agtgaatggc aactctctga taatgctgct tatatgcatt actgtccgaa
     3301 tgaaaccatt gacggtatcg ccatcgacga aacgccaaac ttcggtaaag atgtggtggt
     3361 cgccgccgac ttctcttcaa ccattctttc ccgtccgatt gatgtcagcc gttatggtgt
     3421 aatttacgct ggcgcgcaga aaaatatcgg cccggctggc cttacaatcg tcatcgttcg
     3481 tgaagatttg ctgggcaaag cgaatatcgc ctgcccatcg attctggatt actccatcct
     3541 caacgataac gattccatgt tcaacacgcc gccgacattt gcctggtact tatctggcct
     3601 ggtcttcaaa tggctgaaag cgaacggcgg tgtggttgca atggataaga tcaatcagca
     3661 aaaagcagaa ctgctgtatg aggtgattga taacagcgat ttctaccgca atgatgtggc
     3721 gaaagctaac cgttcgcgga tgaacgtgcc gttccagttg gcggatagcg cgcttgataa
     3781 attgttcctc gaagagtctt tcgctgcggg cttacatgcg ctgaaaggtc accgagtggt
     3841 cggcggaatg cgtgcttcta tttataacgc catgccgctg gaaggtgtta aagcactgac
     3901 agacttcatg gttgagttcg aacgtcgtca cggctaatgt cgaaattttg cataatcccc
     3961 acagccagcc tgtggggttt ttatttctgt tgtagagagt tgagttcatg gaatccctaa
     4021 cgttacaacc catcgctcgt gtcgatggca ctattaatct gcccggttcc aagagcgttt
     4081 ctaaccgcgc tttattgctg gcggcattag cacacggcaa aacagtatta accaatctgc
     4141 tggatagcga tgacgtgcgc catatgctga atgtattaac agcgttaggg gtaagctata
     4201 cgctttcagc cgatcgtacg cgttgcgaaa tcatcggtaa cggcggtcca ttacacgcaa
     4261 aaagtgccct ggagttgttc ctcggtaacg ccggaacggc aatgcgtccg ctggcggcag
     4321 ctctttgtct gggtagcaat gatattgtgc tgaccggtga accgcgtatg aaagaacgcc
     4381 ccattggtca tctggtggat gcgctgcgcc tgggcggggc gaagatcact tacctggaac
     4441 aagaaaatta tccaccattg cgtttacagg gcggctttaa tggcggcaac gttgacgttg
     4501 atggctccgt ttccagccag ttcctcaccg cactgttaat gactgcgcct cttgcaccgg
     4561 aagatacggt gattcgtatt aaaggcgatc tggtttctaa accttatatc gacatcacac
     4621 tcaatctgat gaagacgttt ggtgttgaaa ttgaaaatca gcactatcaa caatttgtcg
     4681 taaaaggggg gcagtcttat cagtctccgg gtacttattt ggtcgaaggc gatgcatctt
     4741 cggcttctta cttcctggca gcagcagcaa tcaaaggcgg cactgtaaaa gtgaccggca
     4801 ttggacgcaa cagtatgcag ggtgatattc gctttgctga tgtgctggaa aaaatgggcg
     4861 cgaccatttg ctggggcgat gattatattt cctgcatgcg gggtgaactg aacgctattg
     4921 atatggatat gaaccatatt cctgatgcgg cgatgaccat tgccacggcg gcgttatttg
     4981 caaaaggcac caccacgctg cgcaatatct ataactggcg tgttaaagag accgatcgcc
     5041 tgtttgcgat ggcaacagaa ctgcgtaaag ttggtgcgga agtcgaagag gggcacgatt
     5101 ttattcgcat tacgccaccg gaaaaactga aatttgctga gatcgccact tataatgatc
     5161 accggatggc gatgtgtttc tcgctggtcg cattatcaga tacggctgtg acgattcttg
     5221 atccaaaatg taccgctaaa acttttccag actatttcga gcaattggcg cggataagtc
     5281 aatccggctg aatgaataac gggcgataac atagacaatc ctttcttttc aaagacgtcg
     5341 gcacattgcc ggcgtttttt ttcggacctt gtggggtatt tttattaatg gtagcgttaa
     5401 ttatcaatgt aacttgttga tacataatgt ttatatataa ttaatcaacg gatgattcac
     5461 atgaagaata ctaaattatt gctggcgatt gcgacctctg cagccttact gacaggatgt
     5521 cagaatacgc acggtattaa taccgatttg gctatcagct caggtctgaa tgcttataaa
     5581 gccgcaacat taagcgatgc tgatgctaaa gcaatcgcca atcagggctg tgtcgaaatg
     5641 gacagcggca ataaagtggc aagcaaatcc agcaagtacg ggaaacgtct ggcaaaaatc
     5701 gccaaagcat tgggtaacaa tattaatggc acgccagtca actataaggt ttatattacc
     5761 agcgacgtca acgcatgggc gatggcgaac ggttgtgttc gtgtctacag tggcctgatg
     5821 gacatgatga acgacaacga aattgaaggc gttctgggcc atgaactggg ccacgtcgcg
     5881 ttgggtcact cgctggctga aatgaaagct tcttatgcga tcgtggccgc acgcgatgcc
     5941 atttcagcta ccagcggtgt ggcttcccag ctttcccgct cacaattggg tgatatcgca
     6001 gaaggcgcta tcaatgctaa atactcccgt gataaagaat ccgaagcaga tgatttctct
     6061 tttgatctgt tgaagaaacg tggcatcagt acccaggggc tggttggtag ctttgaaaaa
     6121 ctggccagcc tggatggcgg tcgcacccag tccatgtttg attctcaccc accatcaaca
     6181 gagcgtgcgc aacacatccg tgatcgtatc gcctctggta agtaaatcat tgtcatcttt
     6241 cgggctggtc ttctgccagc ccgctataat tgcgcaataa atccccatct gaatacagac
     6301 aaaactggtt tttgcacaca acgttaacga tttgtggcgt cggcgcgtat aatgcgcgcg
     6361 gttatgttaa cggtacgcct gttttaagga gataaagatg acggcaattg ccccggttat
     6421 taccattgat ggcccaagcg gtgcagggaa aggcaccttg tgtaaggcta tggcggaagc
     6481 gttgcaatgg catctgctgg attcgggtgc aatttatcgc gtactggcac tagcggcatt
     6541 acatcaccat gttgatgttg catcggaaga tgcgctggtg ccgctggcat cccatctgga
     6601 tgtacgtttt gtgtcaacca acggtaatct ggaagtgatc ctcgaagggg aagatgtcag
     6661 cggcgaaatt cgtactcagg aggtggcgaa tgctgcttca caagtcgcgg cattcccacg
     6721 cgttcgtgaa gcattattgc gtcgccaacg cgcgtttcgc gaattaccag gcctgattgc
     6781 cgatgggcgc gacatgggaa cggtggtatt ccctgatgcg ccagtgaaaa ttttccttga
     6841 cgcctcctcg gaagaacgtg cgcatcgccg catgctacag ttgcaggaga agggctttag
     6901 tgttaacttt gagcgccttt tggccgagat caaagaacgc gacgaccgcg atcgtaaccg
     6961 agcggtagcg ccactggttc cggcagccga tgctttagtg ttggattcca ccaccttaag
     7021 cattgagcaa gtgattgaaa aagcgctaca atacgcgcgc cagaaattgg ctctcgcata
     7081 agcgaccgaa tttgcagtac ccccgttgca atggaatgac agcgggtatg ttaaacaacc
     7141 ccatccggca tggagccagg tggacgttaa atataaacct gaagattaaa catgactgaa
     7201 tcttttgctc aactctttga agagtcctta aaagaaatcg aaacccgccc gggttctatc
     7261 gttcgtggcg ttgttgttgc tatcgacaaa gacgtagtac tggttgacgc tggtctgaaa
     7321 tctgagtccg ccatcccggc tgagcagttc aaaaacgccc agggcgagct ggaaatccag
     7381 gtaggtgacg aagttgacgt tgctctggac gcagtagaag acggcttcgg tgaaactctg
     7441 ctgtcccgtg agaaagctaa acgtcacgaa gcctggatca cgctggaaaa agcttacgaa
     7501 gatgctgaaa ctgttaccgg tgttatcaac ggcaaagtta agggcggctt cactgttgag
     7561 ctgaacggta ttcgtgcgtt cctgccaggt tctctggtag acgttcgtcc ggtgcgtgac
     7621 actctgcacc tggaaggcaa agagcttgaa ttcaaagtaa tcaagctgga tcagaagcgc
     7681 aacaacgttg ttgtttctcg tcgtgccgtt atcgaatccg aaaacagcgc agagcgcgat
     7741 cagctgctgg aaaacctgca ggaaggcatg gaagttaaag gtatcgttaa gaacctcact
     7801 gactacggtg cattcgttga tctgggcggc gttgacggcc tgctgcacat cactgacatg
     7861 gcctggaaac gcgttaagca tccgagcgaa atcgtcaacg tgggcgacga aatcactgtt
     7921 aaagtgctga agttcgaccg cgaacgtacc cgtgtatccc tgggcctgaa acagctgggc
     7981 gaagatccgt gggtagctat cgctaaacgt tatccggaag gtaccaaact gactggtcgc
     8041 gtgaccaacc tgaccgacta cggctgcttc gttgaaatcg aagaaggcgt tgaaggcctg
     8101 gtacacgttt ccgaaatgga ttggaccaac aaaaacatcc acccgtccaa agttgttaac
     8161 gttggcgatg tagtggaagt tatggttctg gatatcgacg aagaacgtcg tcgtatctcc
     8221 ctgggtctga aacagtgcaa agctaacccg tggcagcagt tcgcggaaac ccacaacaag
     8281 ggcgaccgtg ttgaaggtaa aatcaagtct atcactgact tcggtatctt catcggcctg
     8341 gacggcggca tcgacggcct ggttcacctg tctgacatct cctggaacgt tgcaggcgaa
     8401 gaagcagttc gtgaatacaa aaaaggcgac gaaatcgctg cagttgttct gcaggttgac
     8461 gcagaacgtg aacgtatctc cctgggcgtt aaacagctcg cagaagatcc gttcaacaac
     8521 tgggttgctc tgaacaagaa aggcgctatc gtaaccggta aagtaactgc agttgacgct
     8581 aaaggcgcaa ccgtagaact ggctgacggc gttgaaggtt acctgcgtgc ttctgaagca
     8641 tcccgtgacc gcgttgaaga cgctaccctg gttctgagcg ttggcgacga agttgaagct
     8701 aaattcaccg gcgttgatcg taaaaaccgc gcaatcagcc tgtctgttcg tgcgaaagac
     8761 gaagctgacg agaaagatgc aatcgcaact gttaacaaac aggaagatgc aaacttctcc
     8821 aacaacgcaa tggctgaagc tttcaaagca gctaaaggcg agtaattctc tgactcttcg
     8881 ggatttttat tccgaagttt gttgagttta cttgacagat tgcaggtttc gtcctgtaat
     8941 caagcactaa gggcggctac ggccgccctt aatcaatgca gcaacagcag ccgcttaatt
     9001 tgcctttaag gaaccggagg aatcatgacc aagtcagaat tgatagaaag acttgccacc
     9061 cagcaatcgc acattcccgc caagacggtt gaagatgcag taaaagagat gctggagcat
     9121 atggcctcga ctcttgcgca gggcgagcgt attgaaatcc gcggtttcgg cagtttctct
     9181 ttgcactacc gcgcaccacg taccggacgt aatccgaaga ctggcgataa agtagaactg
     9241 gaaggaaaat acgttcctca ctttaaacca ggtaaagaac tgcgcgatcg cgccaatatt
     9301 tacggttaag tttttactca aacttgaacg cgagaaaagc acctgtcggg tgcttttttc
     9361 atttctctaa tctggaactg gaagctgcct cgcagagttt tgagcagttt tcaccctatc
     9421 gttaaattct tctgaacgtg cctcggggaa cgcaaaactc ccaccagaca accgttcaac
     9481 aaagttgcac actttctata aacagggagg gtgcatgaaa ataacgacag tcggcgtatg
     9541 cataatttgc ggaatttttc cgttgctgat tttgccccaa ttgcctggga cagtaaccct
     9601 tgcgtttctg actctcttcg cctgtgtact ggcatttatc cctgttaaaa ccgtccgtta
     9661 tatcgtgctg acgttgctgt ttttcgtttg gggcatatta gcagcaaagc aaattttgtg
     9721 ggcaggagaa accttaactg gcgcgacgca ggatgcaatt gttgagatca ttgctactga
     9781 cggcatgacc actcattacg gtcaaattac tcatctacaa ggtcgacgta tattccctgc
     9841 gccaggcctc gtactgtatg gcgaatatct tccgcaagcg gtttgtgccg gacaagtatg
     9901 gtcaatgaaa ctcaaagttc gtgcagttca tggtcaactt aatgatggcg gctttgatag
     9961 ccagcgttat gccattgccc agcatcaacc gctcaccggc cgttttctgc aggcaagtgt
    10021 cattgaaccg aattgtagcc tgcgtgcaca gtatctggcg tcactacaaa caacgctgca
    10081 accctatatg tggaacgcgg ttattcttgg attaggtatg ggggaacggt tatccgtccc
    10141 caaagaaatc aaaaatatca tgcgtgatac tggaacggcg catttaatgg cgatatcagg
    10201 attgcacatc gcttttgcgg cgttgctggc tgcaggactc attcgcggtg ggcaagtttt
    10261 tctgcctggg tgctggatcc actggcaaat gccattaatt ggtggaatct gctgtgctgc
    10321 tttttatgcc tggcttacgg gaatgcaacc tcctgcattg cgtaccgtgg tggcgcttgc
    10381 catttgggga atgcttaagt taagtggacg acaatggagt ggctgggatg tatggatatg
    10441 ttgtctggcg gcaattttgc tgatggatcc tgttgccatt ctctcgcaaa gtttatggct
    10501 ctctgccgct gcggtcgcgg cactgatttt ttggtatcag tggtttccct gtccggagtg
    10561 gcagctgccg ccggtattgc gcgcggttgt ttccctcatc catctgcaac tgggaatcac
    10621 cctcctgctt atgcccgtgc aaattgtcat atttcatggc attagtctga cctcgtttat
    10681 tgcaaatctt tttgcaattc ccctggtgac atttatcacg gttccgttga ttctcgccgc
    10741 gatggttgtg catttaagcg ggccgtttat tctggaacaa gggttatggt ttcttgccga
    10801 ccggtctttg gctttacttt tctgggggtt aaagagtttg ccggaagggt ggatcaacat
    10861 tgctgaacgt tggcaatggc taacattttc cccatggttc ttactggtgg tatggcgatt
    10921 aaacgcctgg cgaacgttgc cagcaatgtg tgtggctgta ggcttgctga tgtgcttgcc
    10981 gctgtggcaa aaacctcgac ctgacgagtg gcaagtgtac atgcttgatg tcgggcaagg
    11041 gctggcaatg gtcattgcgc gaaacggcaa agcgattctc tatgacacgg gactggcctg
    11101 gcccgaaggg gatagtgggc aacaactgat tatcccctgg ctccactggc ataatcttga
    11161 accggaaggc gttattttga gtcatgaaca tctggatcac cggggagggc tggactcaat
    11221 attgcacaca tggccgatgt tatggatccg aagtccgtta aactgggaac atcatcagcc
    11281 ctgtgtgcgt ggcgaagcgt ggcaatggca aggattgcgt ttcagcgcgc actggccttt
    11341 acaaggtagc aacgataaag gaaataacca ttcctgtgtg gttaagattg atgacgggac
    11401 gaatagcatt cttctaaccg gtgatattga agcccccgct gaacaaaaga tgctaagccg
    11461 ttactggcag caaatgcagg caacattact tcaggtacct caccatggca gtaatacctc
    11521 atcatcgttg ccattaattc agcgagtgaa tggaaaagtg gcactcgcat cggcatcgcg
    11581 ctataacgca tggcgactgc cctcaagcaa agttaaacat cgctatcaac aacagggcta
    11641 taagtggctt gatactccac atcagggtca aataacggtc aatttttcag cgcaaggctg
    11701 gcggattagc agcctcagag agcaaatttt acctcgttgg tatcatcagt ggtttggcgt
    11761 gccagtggat aacgggtaga atatgcggct atttcaacaa atgctggttt tttgaatgca
    11821 taacgacaaa gatctctcta cgtggcagac gttccgccga ctgtggccaa ccattgcgcc
    11881 ttttaaagcg ggtctgatcg tggcgggcgt agcgttaatc ctcaacgcag ccagcgatac
    11941 cttcatgtta tcgctcctta agccacttct tgatgatggc tttggtaaaa cagatcgctc
    12001 cgtgctgatg tggatgccgc tggtggtgat cgggctgatg attttacgtg gtatcaccag
    12061 ctatatctcc agctactgta tctcctgggt atcaggaaag gtggtaatga ctatgcgtcg
    12121 ccgcctgttt ggtcacatga tgggaatgcc ggtttcattc tttgacaaac agtcaacggg
    12181 tacgctgttg tcacgtatca cctacgattc cgaacaggtc gcttcttctt cttccggcgc
    12241 actgattact gttgtgcgtg aaggtgcgtc gatcatcggc ctgttcatca tgatgttcta
    12301 ttacagttgg caactgtcga tcattttgat tgtgctggca ccgattgttt cgattgcgat
    12361 tcgcgttgta tcgaagcgtt ttcgcaacat cagtaaaaac atgcagaaca ccatggggca
    12421 ggtgaccacc agcgccgaac aaatgctgaa aggccataaa gaagtattga ttttcggtgg
    12481 tcaggaagtg gaaacgaaac gcttcgataa agtcagtaac cgaatgcgtc ttcaggggat
    12541 gaaaatggtt tcagcctctt ctatctctga tccgatcatt cagctgatcg cctctttggc
    12601 gctggcgttt gttctgtatg cggcaagctt cccaagtgtc atggatagcc tgactgccgg
    12661 tacgattacc gttgttttct cttcaatgat tgcacttatg cgtccgctga aatcgctgac
    12721 caacgttaac gcccagttcc agcgcggtat ggcggcttgt cagacgctgt ttaccattct
    12781 ggacagtgag caggagaaag acgaaggtaa gcgcgtgatc gagcgtgcga ctggcgacgt
    12841 ggagtttcgc aatgtcacct ttacttatcc gggacgtgat gtgcctgcat tgcgtaacat
    12901 caacctgaaa attccggcag ggaagacggt tgctctggtt ggacgatctg gttcaggtaa
    12961 atcaaccatc gccagcctaa tcacacgttt ttacgatatt gatgaaggcg aaatcctgat
    13021 ggatggtcac gatctgcgcg agtataccct ggcgtcgtta cgtaaccagg ttgctctggt
    13081 gtcgcagaat gtccatctgt ttaacgatac ggttgccaac aacattgctt acgcaaggac
    13141 tgaacagtac agccgtgagc aaattgaaga agctgcgcgt atggcctacg ccatggactt
    13201 catcaataag atggataacg gtctcgatac agtcattggt gaaaacggcg tgttgctctc
    13261 tggcggtcag cgtcagcgta ttgctatcgc tcgagccttg ttgcgtgata gcccgatact
    13321 gattctggac gaagctacct cggcgttgga taccgaatcc gaacgtgcga ttcaggcggc
    13381 actggatgag ttgcagaaaa accgtacctc tctggtgatt gcccaccgct tgtctaccat
    13441 tgaaaaggca gacgaaatcg tggttgtcga ggatggtgtc attgtggaac gcggtacgca
    13501 taacgatttg cttgagcacc gtggcgttta cgcgcaactt cacaaaatgc agtttggcca
    13561 atgatcgaaa aaatctggtc tggtgaatcc cctttatggc ggctattgct gccactctcc
    13621 tggttgtatg gcctggtgag tggcgcgatc cgtctttgct ataaactaaa actgaagcgc
    13681 gcctggcgtg cccccgtacc ggttgtcgtg gttggtaatc tcaccgcagg cggcaacgga
    13741 aaaaccccgg tcgttgtctg gctggtggaa cagctgcaac agcgcggtat tcgcgtgggg
    13801 gtcgtatcgc ggggatatgg tggtaaggct gaatcttatc cgctgttatt gtcggcagat
    13861 accaccacag cacaggcggg tgatgaacct gtgttgattt atcaacgcac tggcgcacct
    13921 gttgcggttt ctcccgtgcg ttctgacgcg gtaaaagcca ttctggcgca acaccctgat
    13981 gtgcagatca tcgtaaccga cgatggttta cagcattatc gtctggcgcg tgatgtggaa
    14041 attgtcgtta ttgatggtgt gcgtcgcttt ggcaatggct ggtggttgcc ggcggggcca
    14101 atgcgtgagc gagcggggcg cttaaagtcg gttgatgcgg taatcgtcaa cggcggtgtc
    14161 ccccgcagcg gtgaaatccc catgcatctg ctgccgggtc aggcggtgaa tttacgtacc
    14221 ggtacgcgtt gtgacgttgc tcagcttgaa catgtggtgg cgattgcggg gattgggcat
    14281 ccgccgcgct tttttgccac gctgaagatg tgcggcgtac aaccggaaaa atgtgtaccg
    14341 ctggccgatc atcagtcttt gaatcatgcg gatgtcagcg cgttggtaag cgccggacaa
    14401 acgctggtaa tgactgaaaa agatgcggtg aaatgccggg cctttgcaga agaaaattgg
    14461 tggtatttgc ctgtagacgc acagctttca ggtgatgaac cagcgaaact gcttgcgcaa
    14521 ctaacctcgc tggcttctgg ccactagtta cgccgcggca gcgttcgatt gatggagtca
    14581 tgaatgtcgc tgccgcacct ctcccttgct gatgcgcgta atcttcacct tgccgcgcaa
    14641 ggcctgttaa acaaaccccg ccgtcgagcg tcgttggagg atattccggc aacgatctcc
    14701 cgcatgtcct tgctgcaaat cgataccatc aatattgttg tccgtagccc atatctggtg
    14761 cttttcagcc gtctgggaga ttatcctgcg cagtggctgg atgagtctct ggcgcgtgga
    14821 gaattaatgg aatactgggc gcatgaagcc tgctttatgc cgcgtagcga ttttcgtctt
    14881 attcgccacc gcatgctggc acctgaaaaa atgggctgga aatataaaga cgcctggatg
    14941 caggaacatg cggcggaaat tgcacagtta attcagcata ttcatgataa ggggccagta
    15001 cgttcagccg attttgagca tcctcgtaaa ggggcgagcg gctggtggga gtggaagccg
    15061 cataaacgac atctggaagg tttatttact gccggaaagg tgatggtgat tgaacggcgc
    15121 aacttccagc gcgtttatga tttaactcgc cgtgtcatgc ctgagtggaa tgatgagcgc
    15181 gatctcgttt cgcaagcaga agcagaaatc atcatgctgg ataacagtgc gcgtagcctg
    15241 ggaatatttc gcgaacagtg gctggcagat tactaccggc taaaacgtcc ggcactggcg
    15301 gcgtggcgtg aagcgagggc tgaacagcag caaatcattg ctgtgcatgt tgaaaaattg
    15361 ggtaatcttt ggctgcatgc tgatttgctg ccgctactcg agcgtgcgct cgccggaaag
    15421 ctcactgcaa cgcacagcgc ggtactttcg ccttttgatc ctgttgtctg ggatcgcaaa
    15481 cgcgcggagc agctttttga ttttagctac cggctggagt gctatatccc agcgccgaaa
    15541 cgccagtatg gctattttgt tctgccgtta ttacatcgtg ggcaattagt tgggcgaatg
    15601 gatgccaaaa tgcatcgcca gacaggcatc cttgaagtta tctctctgtg gttacaggaa
    15661 ggcattaaac cgacgacaac gctgcaaaaa gggttacgtc aggcgattac tgatttcgct
    15721 aactggcagc aggcaacgcg ggtgacatta ggacgctgct cgcaaggcct ctttactgat
    15781 tgccgcgccg gctgggaaat agaccccgtc gcataaatga ttatgataag atttatgcat
    15841 tcataacccg atccatctgg aggaactatg gatcatcgtc tgcttgaaat cattgcctgc
    15901 ccggtttgca acggaaaact ttggtataac caggaaaaac aagagcttat ttgcaaactg
    15961 gacaacctgg ctttcccgct gcgcgatggc attccagtgt tgctggaaac ggaagcccgc
    16021 gtgctgactg ctgatgagag taaatcatga gttttgtggt cattattccc gcgcgttacg
    16081 cgtccacgcg tctgcccggt aaaccattgg ttgatattaa cggcaaaccc atgattgttc
    16141 atgttcttga acgcgcgcgc gaatcaggtg ccgagcgcat catcgtggca accgatcatg
    16201 aggatgttgc ccgcgctgtt gaagccgctg gcggcgaagt atgtatgacg cgcgccgatc
    16261 atcagtcagg tacagaacgt ctggcggaag ttgtcgaaaa atgcgcattc agcgacgaca
    16321 ctgttatcgt taatgtgcag ggtgatgaac cgatgatccc tgcgacaatc attcgtcagg
    16381 ttgctgataa cctcgctcag cgtcaggtgg gtatggcgac tctggcggtg ccaatccaca
    16441 atgcggaaga agcgtttaac ccgaatgcgg tgaaagtggt tctcgacgcc gaagggtacg
    16501 cactgtactt ctctcgcgcc accattccgt gggatcgtga tcgttttgca aaagaccttg
    16561 aaagcgtagg cgataacttc ctgcgtcatc ttggtattta tggctaccga gcaggcttta
    16621 tccgtcgtta tgttacctgg cagccaagtc cgttagaaca tatcgaaatg ttagagcagc
    16681 ttcgtgttct gtggtacggc gaaaaaatcc acgttgctgt cgcccaggaa gttcctggca
    16741 caggtgtgga tacccctgaa gatctcaagc gcgttcgcgc cgaaatgcgc taatttcact
    16801 tcacgacact ccagccaatt ttgggaggag tgtcgtaccg ttacgatttt cctcaatttt
    16861 tcttttcaac aattgatctc attcaggtga catcttttat attggcgctc attatgaaag
    16921 cagtagcttt tatgagggta atctgaatgg aacagctgcg tgccgaatta agccatttac
    16981 tgggcgaaaa actcagtcgt attgagtgcg tcaatgaaaa agcggatacg gcgttgtggg
    17041 ctttgtatga cagccaggga aacccaatgc cgttaatggc aagaagcttt agtgcgcccg
    17101 gaaaagcccg acaactggca tggaaaacca ccatgctggc aagaagtggg actgtccgta
    17161 tgccgactat ttatggtgtg atgacgcatg aagaacaccc cggccccgat gtcctgctac
    17221 tggagcggat gcgtggtgtt tcggtggagg caccagcccg aacaccagaa cgctgggaac
    17281 agctcaaaga ccaaatcgtt gaagccttac tggcctggca ccgtcaggac agtcgcggtt
    17341 gcgtcggcgc ggtcgacaat actcaggaaa atttctggcc ctcatggtac cggcaacatg
    17401 ttgaagtgct atggaccacg ctcaatcagt tcaataacac cggtctaacg atgcaggata
    17461 agcggatcct gtttcgcact cgcgaatgtc tcccggcatt atttgaaggc tttaacgaca
    17521 attgtgtact gattcacggt aacttctgtt tacgtagcat gctgaaagat tcgcgcagcg
    17581 atcagttact ggcgatggtc ggaccgggac taatgctttg ggcaccgcga gaatacgaac
    17641 tgttccgact aatggataat tctctggcgg aagatttgct ctggagttat ctgcaacgcg
    17701 cgccagtggc ggagtcgttc atctggcggc gttggttgta tgtgttatgg gatgaagttg
    17761 cgcaactggt caacaccgga cgatttagtc ggcgtaactt cgatctggca tcaaaatcac
    17821 tcttgccgtg gctcgcctga cgaacctttc agccactgcc agattcggcc aagcgtttca
    17881 tagccaacgc ggtcgctatg catcaaccaa accggagaag ggattgcccg ttcccacgga
    17941 ttaagcggcg agtcgatggc caactgattc gccggggctg gtagcggatt taacccttcc
    18001 tgctgaaaaa agatcatcgc ccgcggcaga tgtgatgcgg aggtcaccag caggaaaggt
    18061 gcgtcaccaa tcgcctgttt cactgctgca gcttcttctt cggtatcttt tggcaaatcc
    18121 agggtgataa tttgcttgcg cggcacgccc agcgattgcg caactctggc acctacttcg
    18181 gctgtgctca ccgtattggt tttagctacg cctcccgtaa agatcagttt tgatcccgga
    18241 ttttcgcgcc ataagcgaat accttcattc aggcgtggca gactgttatt gattaaatta
    18301 gagctcggcg cccactgcgg gttccaggta taaccacctc cgagcaccac gatatagtcc
    18361 actttttgcg aattattcca tgtcggataa gtgctttcga tgggacgcaa caggcggtcg
    18421 gctatcggtt gcaaactcaa taacaagagc gccagccacc caatgctaat aaaaatcttc
    18481 ccggtttttt gaaaacggct aaaccacagc agcgccaggc cagcgccaat aatcaacaac
    18541 atcagcggaa gtggcagcaa catattgcca atcacttttt tcagtgtaaa aagcatcctc
    18601 tctggttcct tttttgacca tatagcaagg gatcgtcagc gatattacac cagacgggtt
    18661 cattctcgcc gtggctgtga caaaatagcg tctttatcag aaaacgtctt agccaaccgg
    18721 tggagaaagc aatgcaggat cgcaattttg atgatattgc ggaaaagttt tcccgtaaca
    18781 tttacggcac caccaaaggg cagcttcgac aggctattct gtggcaggat ctcgatcgcg
    18841 tgctggcgga aatgggcccg caaaaactgc gtgtgctgga tgctggcggc ggagaagggc
    18901 agaccgcaat caaaatggcc gagcgcgggc atcaggtcat tttatgcgat ctttctgcgc
    18961 agatgatcga ccgcgcaaaa caggcggcag aagcaaaagg tgtgagcgac aacatgcaat
    19021 ttatacattg cgccgctcag gatgttgctt cgcatttgga aacgcccgtt gatctgatat
    19081 tgttccatgc ggtgctcgag tgggtggccg atccccgcag cgtattacag accctgtggt
    19141 cagtgttgcg tccaggcggc gtgttgtcgt taatgttcta caatgcgcac ggcttgctga
    19201 tgcataacat ggtcgccggg aattttgatt acgtgcaggc gggaatgccg aaaaagaaaa
    19261 aacggacgct ttcgccagat tatccacgcg acccggcgca ggtttatctg tggctggaag
    19321 aagctggttg gcaaattatg ggtaagacag gcgttcgtgt gtttcatgat tatctgcgcg
    19381 agaagcacca gcagcgcgac tgctacgaag cattacttga attagaaacg cgttattgcc
    19441 gtcaggaacc gtatattacc ctggggcgtt atattcatgt caccgcgcgc aaaccgcaga
    19501 gcaaggataa agtatgagtg aattttccca gacagtcccc gaactggttg cctgggccag
    19561 aaaaaatgac ttctccatct cgctgccggt agaccgactc tcttttctgc tggcggttgc
    19621 cacgctgaac ggcgagcgtc tggatggtga gatgagtgaa ggcgagctgg tggatgcatt
    19681 ccgccatgtg agtgatgcgt ttgagcaaac cagcgaaacc atcggcgtgc gcgccaacaa
    19741 cgcgatcaac gacatggtgc gtcaacgtct gctgaaccgc tttaccagcg agcaggcgga
    19801 agggaacgca atttaccgtc tgacgccgct cggcatcggc attactgact attacatccg
    19861 tcagcgcgag ttttctacgc tgcgtctttc tatgcagttg tcgattgtgg cgggtgagct
    19921 aaaacgcgca gcagatgccg ccgaagaggg cggtgatgaa tttcactggc accgtaatgt
    19981 ctatgcgcca ctgaaatatt cggtagcaga aattttcgac agtattgacc tgacgcaacg
    20041 tctgatggac gaacagcagc agcaggtgaa agatgatatc gcccagttgt tgaacaaaga
    20101 ctggcgggcg gcgatctcca gctgtgaatt gttgctttca gaaacttccg gaacgctgcg
    20161 tgaattgcag gatacgctgg aagcggcagg cgacaaattg caggctaatc tgttgcgcat
    20221 tcaggatgcg acgatgaccc atgacgatct gcattttgtc gatcgtctgg tgttcgatct
    20281 gcagagcaaa cttgaccgta ttatcagttg gggccagcaa tccatcgact tgtggattgg
    20341 ctacgaccgc cacgtacaca aatttatccg taccgcaatc gatatggata aaaaccgcgt
    20401 ctttgctcag cggttacgtc agtcggtaca aacctatttt gatgagccgt gggcgctaac
    20461 ttatgccaat gccgatcgtc tgctggatat gcgtgacgaa gagatggtac tgcgcgatga
    20521 agaagtgact ggggaacttc ctgaggatct ggagtacgaa gagtttaacg agatccgcga
    20581 acagctggcg gcgatcatcg aagaacaact tgccgtgtac aaaaccagac aagtgccgtt
    20641 ggatcttggt ctggtggtac gcgaatatct gtcacagtat ccgcgtgcac gtcactttga
    20701 cgttgcgcgc attgttattg atcaggcggt acgtcttggc gtagcgcaag cagatttcac
    20761 cggactgcca gcgaaatggc agccgattaa tgattacgga gccaaggtac aggcgcatgt
    20821 catcgacaaa tattgaacaa gtgatgccgg ttaagctggc gcaggcgctg gcgaatccgt
    20881 tatttccggc gctggatagc gccttacgtt caggccgtca tattggcctc gacgaactgg
    20941 ataatcatgc attcctgatg gattttcagg aatatctgga agagttttac gcgcgttata
    21001 acgttgagct tattcgtgca ccggaagggt tcttctatct acgcccacgt tccaccacgc
    21061 tgatccctcg ttccgtcttg tcggaactgg atatgatggt cgggaaaatc ctctgttatc
    21121 tctatctcag cccggaacgg ctggcgaatg aggggatttt tactcaacag gaactgtacg
    21181 acgaactgct caccctggcc gatgaagcga aactgctgaa actggtgaac aaccgttcaa
    21241 ccggttcaga cgttgaccgt cagaagttgc aggaaaaagt acgttcttcg ctcaaccgtc
    21301 tgcgtcgttt aggcatggtg tggtttatgg gccacgacag cagcaagttc cgcattaccg
    21361 aatcggtgtt ccgcttcgga gccgatgtgc gtgctggcga cgatccccgt gaagcacagc
    21421 gtcgcctgat tcgtgatggt gaagcaatgc cgattgaaaa tcatctgcaa ctcaacgatg
    21481 aaaccgaaga gagtcagcca gatagcggag aggaagaata atgattgaac gcggtaaatt
    21541 tcgctcactg acgctgatta actggaacgg cttttttgcc cgaacttttg accttgacga
    21601 gctggtcacg acgctttctg gcggtaacgg ggcgggtaaa tccaccacca tggcggcgtt
    21661 cgttacggcg cttatccccg atctgaccct gctgcatttc cgtaacacta cggaagccgg
    21721 ggccaccagc ggttcgcgcg ataaaggtct gcacggtaag ctgaaagcgg gtgtctgtta
    21781 ttcgatgctc gacaccatta actcgcgcca ccagcgtgta gtggtcggtg tgcgtctgca
    21841 acaggttgcc ggacgcgatc gtaaagtgga tatcaaacca tttgccattc agggcctgcc
    21901 gatgtcggtg cagccgacac aactggtgac cgaaactctg aacgaacgcc aggcgcgcgt
    21961 gctgccgctt aatgagctga aagacaagct cgaggcgatg gaaggcgtgc agtttaagca
    22021 gttcaactcc attaccgatt accactcgct gatgttcgat ctgggcatca tcgcgcgtcg
    22081 tctgcgctca gcgtctgacc gtagtaaatt ctatcgtctg atcgaagcct cgctgtatgg
    22141 cgggatctcc agcgccatta ctcgttctct gcgcgactac ctgttgccgg aaaacagcgg
    22201 ggtgcggaaa gcgttccagg acatggaagc ggcgctgcgt gaaaaccgca tgacgctgga
    22261 agcgattcgt gtcacccagt cagaccgtga cctgtttaag catctgatca gcgaagccac
    22321 caactacgtg gcggcggact acatgcgtca cgccaacgag cgccgtgttc atctcgataa
    22381 ggccctggag tttcgtcgcg agctacatac ttcgcgtcag caactggcgg ctgagcagta
    22441 caaacacgtc gacatggcgc gagagctggc tgagcacaac ggtgccgaag gcgatctgga
    22501 agcggattat caggcggcca gcgatcacct gaacctggtg caaaccgcac tgcgtcagca
    22561 ggagaagatc gaacgctacg aagcggatct cgatgagctg cagatccgtc tggaagagca
    22621 aaacgaagtg gtggcagaag ccatcgaacg ccaggaagag aacgaggctc gtgcggaagc
    22681 tgccgaactg gaagtggacg agctgaaaag ccagcttgct gactaccagc aggcgctgga
    22741 cgtccagcaa acgcgcgcga tccagtataa ccaggcgatt gctgcgctta atcgtgccaa
    22801 agaactgtgc catctgccgg acttaaccgc cgacagcgct gccgaatggc tggaaacctt
    22861 ccaggcgaaa gagctggaag cgaccgaaaa aatgctctct cttgagcaga aaatgagcat
    22921 ggcgcaaacc gcgcacagcc agtttgagca ggcttatcag ctggtggtgg caatcaacgg
    22981 cccgctggcg cgtaacgagg cgtgggatgt cgctcgcgaa ctattgcgcg aaggggtcga
    23041 tcagcgtcac ctggcagagc aggttcagcc gttgcggatg cgcttaagcg aactggaaca
    23101 gcgtctgcgt gagcagcaag aagctgagcg tctgctggca gatttctgca aacgtcaggg
    23161 caagaatttt gatattgatg aactggaagc tctgcatcag gagctggaag cacgcattgc
    23221 ctctctttcc gatagcgtct ctaacgcccg agaagagcgg atggcactgc gccaggagca
    23281 ggaacagctg cagtctcgca ttcagagttt gatgcagcgt gcgccggttt ggctggcagc
    23341 gcaaaacagt ctcaaccagt tgagcgaaca gtgcggcgaa gagttcacct ccagccagga
    23401 tgtcaccgaa tttctgcaac agttgctgga gcgtgagcgc gaggcaattg ttgaacgcga
    23461 tgaagtaggc gcgcgcaaaa acgccgtcga tgaagagatc gaacgtttaa gccagccagg
    23521 tggttctgaa gatcagcgcc tgaacgcgct ggcggagcgt tttggtggtg tgctgctgtc
    23581 agaaatttat gacgacgtta gcctggaaga tgcgccgtac ttctcagcgc tgtatggccc
    23641 gtcacgccac gccatcgtgg tgccagatct gtcacaggta accgaacacc tggaaggctt
    23701 gaccgattgc ccggaagatc tctatctgat cgaaggggat ccgcagtcat tcgatgacag
    23761 cgtgttcagc gttgatgagc tggaaaaagc ggtggtggtg aaaatcgccg atcgtcagtg
    23821 gcgttattca cgtttcccgg aagtgccgct gtttggtcgt gctgcgcgtg aaagccgtat
    23881 tgaaagcctc catgccgagc gtgaagtgct ttccgaacgc ttcgccacgc tctcctttga
    23941 tgtacagaaa acccagcgtc tgcatcaggc gttcagccgc tttatcggca gtcatctggc
    24001 ggtggcgttt gagtctgacc cggaagcaga aatccgtcaa ctgaacagcc gtcgcgtcga
    24061 actggaacgg gcgttgagta atcatgaaaa tgataaccag cagcagcgta ttcagtttga
    24121 gcaggcgaaa gagggcgtta cggcgctgaa ccgcattctg ccgcgtctca acctgttggc
    24181 tgatgacagc ctggcggatc gcgtcgatga aatccgcgaa cgtctggatg aagcccagga
    24241 agccgcgcgt tttgttcagc agtttggcaa tcaactggcg aaactggaac cgatcgtttc
    24301 ggtattgcag agcgacccgg aacagttcga acagttaaaa gaagattacg cgtactctca
    24361 gcagatgcag cgcgatgccc gtcagcaggc gtttgccctg acggaagtgg tgcagcgtcg
    24421 tgcgcacttt agctactctg actcggcaga aatgcttagc ggtaacagcg atctcaacga
    24481 aaaactgcgt gaacgtctgg aacaggcgga agcggagcgt acccgcgctc gtgaagcgtt
    24541 gcgcggacac gcagtgcagt tgaatcagta caaccaggtg ctggcttcgc tgaaaagttc
    24601 gtatgacacc aaaaaagagc tacttaacga tctgcaacgt gaattgcagg atatcggcgt
    24661 gcgtgctgat agcggggcag aagagcgggc acgtattcgc cgtgacgagc tgcatgcgca
    24721 actgagcaat aaccgttcgc gccgcaatca actggaaaaa gcgctcacct tctgcgaagc
    24781 ggagatggac aacctgaccc gcaaactgcg caagctggag cgtgattact ttgagatgcg
    24841 cgagcaggta gtgaccgcga aagcgggctg gtgtgcagtc atgcgcatgg tgaaagataa
    24901 cggcgttgaa cgccgcttac accgtcgtga actggcttat ctctccgctg atgatttgcg
    24961 ttccatgtcg gataaggcgt taggtgcgct gcgtctggca gtggcagata acgaacatct
    25021 gcgcgatgtg ctgcgcatgt cggaagatcc aaaacgtccg gaacgtaaaa ttcagttctt
    25081 cgtggcggtt tatcagcatc tgcgtgaacg tattcgtcag gatattattc gtaccgatga
    25141 tccggtggaa gctatcgaac agatggagat cgaacttagc cgtctgaccg aagagttaac
    25201 ctcccgcgaa cagaaactgg cgatcagttc ccgcagcgtg gcgaacatta ttcgcaaaac
    25261 cattcagcgc gagcagaacc gtatccgtat gctcaaccag ggcttgcaga gcgtatcgtt
    25321 tggccaggtg aacagcgtgc gtctcaacgt gaacgtgcgt gaaacgcacg ccatgctgct
    25381 ggatgtgctc tctgaacagc atgagcaaca tcaggatctg tttaacagca accgtctgac
    25441 cttctcagaa gcgctggcga aactgtatca gcgtcttaat ccgcagattg atatggggca
    25501 acgcacgccg cagaccattg gcgaagaact gctggattac cgcaactatc tggaaatgga
    25561 agttgaggtt aaccgtggtt ctgatggctg gctgcgcgca gaatctggcg ctctgtcgac
    25621 cggtgaggcg attggtaccg gtatgtcgat tctggtgatg gtggtacaaa gctgggaaga
    25681 tgaatctcgc cgcctgcgcg gtaaagatat ctctccttgc cgcctgctgt tcctcgatga
    25741 agcagcgcga ctggatgctc gttctatcgc cacgctgttt gaattgtgtg aacgtttgca
    25801 aatgcagctc atcatcgcag cgccggaaaa tatcagtccg gaaaaaggca ccacctataa
    25861 actggtgcgt aaagtcttcc agaataccga acacgttcac gtcgtcggcc tgcgaggatt
    25921 tgcgccgcaa ctccctgaaa cgcttccagg aagtgacgaa gcgccttacc aggcgagtta
    25981 aaatgaaggc ggcagcaatg ccgccttttc tttttccgaa aactccgttt ctgcactaaa
    26041 aaagtggcac attacggcgc gcttttcttt aaacttcttt acattaggtt atgtaaaaac
    26101 gtatcggcgt ttatatactg aagataagtc tgatgagtaa caggcttgct cgtcatactt
    26161 tcgtgagtat tggcgttgta caggcaagtc gtaaaataac agcctggcta ttcagagtat
    26221 gataaaaaca gggggcaagg gatgttgctt aatatgatgt gtggtcgtcg gctgtcggca
    26281 atcagtttgt gcctggccgt aacattcgct ccactgttca atgcgcaggc cgatgagcct
    26341 gaagtaatcc ctggcgacag cccggtggct gtcagtgaac agggcgaggc actgccgcag
    26401 gcgcaagcca cggcaataat ggcggggatc cagccattgc ctgaaggtgc ggctgaaaaa
    26461 gcccgcacgc aaatcgaatc tcaattaccc gcaggttata agccggttta tcttaaccag
    26521 cttcaattgt tgtatgccgc acgcgatatg caacccatgt gggaaaaccg tgatgttgtt
    26581 aaagccttcc agcaacagct ggcagaggtg gcgattgccg gtttccagcc gcagtttaat
    26641 aaatgggtag agttactgac cgatcctggt gttaacggga tgacacgcga cgtggtgctc
    26701 tctgatgcga tgatgggcta tctccatttc attgcaaata ttccggtcaa aggcactcgc
    26761 tggctatata gcagtaaacc ttatgcgctt gcaacgccgc cgctttcggt gattaaccaa
    26821 tggcagctgg cgctggataa aggtcaattg ccgacgtttg ttgcaggact ggcaccgcag
    26881 catccgcaat atgcggcgat gcatgaatcg ttactggcct tactcagtga caccaaaccg
    26941 tggccccaac tgaccggcaa agcaacgttg cgcccaggac agtggagtaa cgacgtaccg
    27001 gcgttgcgcg aaatattgca acgtacaggc atgttggacg ggggaccgaa aattacgcta
    27061 cctggcgatg acacgccaac tgacgcggta gtcagcccat ccgctgttac tattgaaaca
    27121 gcagaaacta agccgatgga caagcaaact acgtctcgta gtaaacctgc gcctgtcgtt
    27181 cgtgccgcct acgagaatga actggtggaa gccgttaaac gttttcagac atggcaagga
    27241 ttgggagcag atggcgctat tggcccggca acgcgtgact ggttaaacgt aacgcccgcc
    27301 cagcgtgctg gcgtgttggc actcaacatc cagcgattgc gcttgctgcc aacagagctt
    27361 tctaccggga tcatggttaa cattccggcc tattcgctgg tctactatca gaacggcaat
    27421 caggtgctgg attcgcgagt cattgtcggt cgccccgatc gcaaaacgcc gatgatgagc
    27481 agtgccctta acaacgtggt ggtaaacccg ccgtggaacg tcccaccaac tctggcacgc
    27541 aaagatattc tgccgaaagt gcgcaatgat cctgggtatc tcgaaagcca tggctatacg
    27601 gtgatgcgcg gctggaacag cagagaagcg attgacccat ggcaggttga ctggtctaca
    27661 atcacggcct cgaatttacc gttccgcttc cagcaggctc caggcccacg gaactcgctg
    27721 gggcgctata aattcaatat gccgagttca gaggccattt atttgcatga cacgccgaac
    27781 cacaatctgt tcaagcgtga tacacgcgca ttgagctcag gctgtgtacg agtgaataaa
    27841 gcttccgatc tggcgaatat gctgttgcag gatgcaggct ggaatgacaa acgtatttct
    27901 gatgcgctga agcagggcga tacgcgttac gtcaatattc ggcagtcgat tccggtgaat
    27961 ctctactatc tgacggcctt tgttggtgca gatggtcgta cccagtatcg tacagatatt
    28021 tacaattatg atctgcctgc gcgatccagc tcgcaaattg tatcgaaagc ggaacaatta
    28081 atcaggtaaa tgaagtagtt cggggaaatc agttgtcgta ataattagca tgatcggggg
    28141 gcgattctct gtagcccccg tcactgctgg ggttgagtca tcttgacgtc tgctttacgg
    28201 gcggttaagg tgcctcttgt gcgccagaag tgcatataaa cgataacatt gacctgtaga
    28261 cttgattatc atggacaaat tcgacgctaa tcgccgcaaa ctgctggcgc ttggtggcgt
    28321 tgcactcggt gccgccatcc tgccgacccc ggcgtttgca acactctcta ccccacgccc
    28381 gcgcattttg acactcaata atcttcatac cggagagtca atcaaagcgg agtttttcga
    28441 tggcagaggc tatattcagg aagaattggc aaaacttaac cattttttcc gcgattaccg
    28501 cgcgaacaaa ataaagtcca tcgacccagg attattcgac cagttgtatc gcctgcaagg
    28561 gttgttaggc acccgcaaac cggtgcaact catttccggt tatcgttcca ttgataccaa
    28621 caatgaacta cgtgcccgca gccgtggagt agcgaagaaa agctatcaca ctaaaggcca
    28681 ggcgatggat ttccacattg aaggtatcgc gttaagcaat attcgcaaag ccgcgttatc
    28741 tatgcgcgca ggtggtgtag gatattaccc acgtagtaac tttgtgcata ttgataccgg
    28801 gccagcacgg cactggtagt aatcgcttaa cgaaacaggg gcagtatgaa ctatcgtatt
    28861 attccggtca ccgcattctc ccagaactgt tcattgatct ggtgtgaaca aacccgtctg
    28921 gccgcactgg tcgatcctgg cggcgatgcg gaaaaaatca aacaggaagt tgatgccagc
    28981 ggcctgacac tgatgcagat cctgctgact catggtcatc tggaccacgt tggcgcagcg
    29041 gcggaactgg cgcaacatta cggcgtgccg gtttttggcc cggaaaaaga agatgagttc
    29101 tggctgcaag gcttgcctgc gcaaagtcgt atgtttggtc tggaagagtg ccagccgctg
    29161 acgccagatc gttggctgaa cgaaggcgat accatcagca tagggaatgt gactttacag
    29221 gtgttacatt gccctgggca tacgccgggt catgtcgtgt tttttgatga tcgggcaaag
    29281 ctgctgattt ctggcgacgt tattttcaaa ggtggagtag ggcgcagtga cttcccgcgt
    29341 ggcgatcata atcaactgat ttcttcaatc aaagataaat tgctgccact gggggatgac
    29401 gtgacattta ttccgggtca cggaccatta tccacacttg gttatgagcg cctgcataat
    29461 cccttcctgc aagacgaaat gcccgtctgg taaaggcgca taaaaaagcc cgctttcaag
    29521 cgggcttcat cggttttaac gcttacagca ctgccacaat cgcttcgcac agcggcgcca
    29581 tgttatctgg tgtcatcccg gccacgttta cgcgaccaga agcaacagca tatacgccaa
    29641 actcttcgcg cagacgcagc acttgttctt ttgtcaggcc actgaaggag aacatgccgt
    29701 tctgtttgat gataaagctg aagtcgcgat ttgcgccttt ttcctgcagc gtattgacga
    29761 acaactgacg catacgctga atacgctggc gcatatcagt cagttcttgt tcccaaatcg
    29821 cacgtaacgc atcgttgctc aggatggtgg caacaacaga agcgccgtgt gctggtgggt
    29881 tagagtagtt agcacgaatc gccgctttca tttggctgaa tgcgcgttca acagtttcac
    29941 tgtcagcagc aaccagagta caagcgccaa cacgctcgtt gtacaggcca aagtttttag
    30001 agtaggaact ggcaacaatc agctccttat gcagagccgc gaaagcgcgc agtccttcag
    30061 catcttcttc cagaccacgg gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc
    30121 ctttctcaac ggagagttgt gccagtgtct gccattgttc cagcgtaggg tcgatgccgg
    30181 ttgggttatg gcagcagcca tggaacagca ctacgtcgcc agcctgggct tcgttcaggc
    30241 tgttaatcag tgcatcgaag tcaagagtgt gattttccgc atcataataa gcgtattcac
    30301 gaacttccag acctgcagag ttaaagacgc tcttatggtt cggccagctt gggttgctca
    30361 cccacacacg cttaacgctg gtattttttg ccaggaaatc ggcagccacg cgtagtgcgc
    30421 cagtgccacc cggagtctgt gccgtgcgag cacgtttgtc attgatcagg gcgctacctt
    30481 taccaaacag cagttcctga gtgcagcgac caaattcagg gatgccgtca atgccgaggt
    30541 aatttttggt ggtttcattt tcgagcagat actgttcagc ctttttcacg ctagtcagta
    30601 ccggggtttt acccgtctca tctttataga caccaatccc gaggttaatt ttgccgggac
    30661 gttcatcggc acgaaacaga tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa
    30721 tgttctcaaa catgacgagg ttccattatg gttacagaag ggaagtccgc tatcagggta
    30781 acgggagatt tacaaaattc caactattac tgatgaaaac gcaggctgtt tttgcaagac
    30841 gtgagattgc tctggaaggc ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt
    30901 aacaaagagg tgtgctatta gaactggtaa acgataccca cagcaacggt gtcgtcagaa
    30961 cctacgccca gtttgttgtc agaatcgatc tggttgatga tgtagtcaac ataggtggac
    31021 atgtttttgt tgaaatagta ggttgcgccc acttcaaagt agttcaccag atcaacatca
    31081 ccgatacctt ctacgtcttt cgctttagat ttggtgtaag cgatggacgg acgcagaccg
    31141 aaatcaaact ggtattgcgc aactaacaga acgtcttgcg ttttgttggc gaagccgctg
    31201 gtgtttgtaa atttattagt gatcggcgta gcgttacggg tttcaccgta gttcgctgcc
    31261 aggtagatgt tgttcgcgtc atacttcaga ccagtagccc actgttcagc ttttttgcct
    31321 ttgccaaggg aagactcttc ctgcaggttg gtacggtcag cggcaccata agcaccaacg
    31381 ataccaaagc cttcgtattc gtagctgata gaaccgccaa caccgtcgcc gttagagcgg
    31441 cgtgcagtgt cacgctcgtt tttccccagg tactgaacag caaagttcag accatcaacc
    31501 agaccaaaga agttggagtt acgataggta gcaacgccgc caacacgacc aacgaagaag
    31561 tcatcgctgt atgcagtatc accaccaaat tctggcagca tatcggtgta acccagtgca
    31621 tcataaacca caccgtagtt acggccgtaa tcgaaagaac caacgtcagc gtatttaaga
    31681 cccgcgaatg ccagacgcgt tttgttacca gtttgagcgt cagcgccctc agagttgtta
    31741 ccctggaagt tatattccca ctgaccataa ccggtcagat cggaattgat ttgagtttcc
    31801 cctttaaaac caagacgggc ataggtcatg tcgccattgc caccgtaact gttttcaccg
    31861 ttacccttgg agaaataatg cagaccgaca gctttaccgt acagatctac tttgttgcca
    31921 tctttgttat agatttctgc agcgtttgca gtacctgcta ctaacagagc agggacgatc
    31981 actgccagaa tattgcgctt catcattatt tattaccctc atggtttttt ttatgacacc
    32041 tgccactgcc gtcaataagt tctgtcaata aaaatttacg gaactattga tgagagtttg
    32101 gtgtctttat gtgtctgcag gcatctttcc attcaaacta
//
LOCUS       DAZFJL010000042        31712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFJL010000042 DAZFJL010000000
VERSION     DAZFJL010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.042"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(365..1561)
                     /gene="hemY"
                     /locus_tag="ACHJ8C_001911"
     CDS             complement(365..1561)
                     /gene="hemY"
                     /locus_tag="ACHJ8C_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO5551773.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1564..2769)
                     /gene="hemX"
                     /locus_tag="ACHJ8C_001912"
     CDS             complement(1564..2769)
                     /gene="hemX"
                     /locus_tag="ACHJ8C_001912"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO5551774.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDVLANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQAD
                     APAAAPQGE"
     gene            complement(2791..3531)
                     /gene="hemD"
                     /locus_tag="ACHJ8C_001913"
     CDS             complement(2791..3531)
                     /gene="hemD"
                     /locus_tag="ACHJ8C_001913"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO5551775.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIRDSLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3528..4469)
                     /gene="hemC"
                     /locus_tag="ACHJ8C_001914"
     CDS             complement(3528..4469)
                     /gene="hemC"
                     /locus_tag="ACHJ8C_001914"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO5551776.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4856..7402
                     /gene="cyaA"
                     /locus_tag="ACHJ8C_001915"
     CDS             4856..7402
                     /gene="cyaA"
                     /locus_tag="ACHJ8C_001915"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO5551777.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGTSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRREVLSQLVKEWEWDDSRLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQEHDAPLLQQYFS"
     gene            complement(7442..7762)
                     /gene="cyaY"
                     /locus_tag="ACHJ8C_001916"
     CDS             complement(7442..7762)
                     /gene="cyaY"
                     /locus_tag="ACHJ8C_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO5551778.1"
                     /translation="MNDSEFHRLADQLWLTIEEHLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSDETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7810..9139)
                     /locus_tag="ACHJ8C_001917"
                     /pseudo
     CDS             complement(7810..9139)
                     /locus_tag="ACHJ8C_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            complement(9136..10605)
                     /locus_tag="ACHJ8C_001918"
     CDS             complement(9136..10605)
                     /locus_tag="ACHJ8C_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO5551779.1"
                     /translation="MHYRNVVCLLSCSLFLSSAWGCRLDEPEHNIYQKQGKGVVYLRP
                     YEKTNLSLPQINYKRLRLLPNLLIDPTKLEDWETVPPATNLMTDVVYSGANATLPHYS
                     YYSDGRAILYAGEIVQNPPGTPPVDISSFQAWGDFAADKYSLYYEGKRTDSNQQLNRR
                     TLRQVEFNPQWKPDWLGLILRDKHYLYANGQRLDDPDTFTVLAQKSWDQRGKFSTAFN
                     PCLLAPFGPWDMLARTQTKIMINGEQLDADPDTFSVVRWMPGSLLTWRDKNGLQRKVL
                     DKENLAWDEDLTKHCLDFSLLEKKVFWRQGPDCKQEELPGLDPEQFHPISDAVAQYQD
                     SLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAEGWVNHSSLHVFES
                     DGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYVYSFDGAQLWRYRTADPKQVHLI
                     WKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            10794..10997
                     /gene="yifL"
                     /locus_tag="ACHJ8C_001919"
     CDS             10794..10997
                     /gene="yifL"
                     /locus_tag="ACHJ8C_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5551780.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11034..11858
                     /gene="dapF"
                     /locus_tag="ACHJ8C_001920"
     CDS             11034..11858
                     /gene="dapF"
                     /locus_tag="ACHJ8C_001920"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO5551781.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            11855..12562
                     /gene="yigA"
                     /locus_tag="ACHJ8C_001921"
     CDS             11855..12562
                     /gene="yigA"
                     /locus_tag="ACHJ8C_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFO5551782.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLARWIERV"
     gene            12559..13455
                     /gene="xerC"
                     /locus_tag="ACHJ8C_001922"
     CDS             12559..13455
                     /gene="xerC"
                     /locus_tag="ACHJ8C_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO5551783.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRNLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13455..14171
                     /gene="yigB"
                     /locus_tag="ACHJ8C_001923"
     CDS             13455..14171
                     /gene="yigB"
                     /locus_tag="ACHJ8C_001923"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO5551784.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEQEALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14255..16417
                     /gene="uvrD"
                     /locus_tag="ACHJ8C_001924"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14255..16417
                     /gene="uvrD"
                     /locus_tag="ACHJ8C_001924"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO5551785.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(16462..17364)
                     /locus_tag="ACHJ8C_001925"
     CDS             complement(16462..17364)
                     /locus_tag="ACHJ8C_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFO5551786.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(17447..18211)
                     /gene="yigE"
                     /locus_tag="ACHJ8C_001926"
     CDS             complement(17447..18211)
                     /gene="yigE"
                     /locus_tag="ACHJ8C_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO5551787.1"
                     /translation="MAHRLFIGKGMITLNLKRIFLTLTLLPLFAVAADDCAPSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENGVINPRIHPNVASRKIRNGVGINKQGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            18373..18429
                     /gene="ysgD"
                     /locus_tag="ACHJ8C_001927"
     CDS             18373..18429
                     /gene="ysgD"
                     /locus_tag="ACHJ8C_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO5551788.1"
                     /translation="MDTPSRFWLTILSSRINS"
     gene            18581..19531
                     /gene="corA"
                     /locus_tag="ACHJ8C_001928"
     CDS             18581..19531
                     /gene="corA"
                     /locus_tag="ACHJ8C_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO5551789.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(19660..20547)
                     /gene="rarD"
                     /locus_tag="ACHJ8C_001929"
     CDS             complement(19660..20547)
                     /gene="rarD"
                     /locus_tag="ACHJ8C_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO5551790.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVMVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(20599..21066)
                     /gene="yigI"
                     /locus_tag="ACHJ8C_001930"
     CDS             complement(20599..21066)
                     /gene="yigI"
                     /locus_tag="ACHJ8C_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO5551791.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            21231..22100
                     /gene="pldA"
                     /locus_tag="ACHJ8C_001931"
     CDS             21231..22100
                     /gene="pldA"
                     /locus_tag="ACHJ8C_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO5551792.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYIIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSDESSPFRETNYEPQLFLGFATDYNFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            22225..24054
                     /gene="recQ"
                     /locus_tag="ACHJ8C_001932"
     CDS             22225..24054
                     /gene="recQ"
                     /locus_tag="ACHJ8C_001932"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO5551793.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQELCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            24118..24738
                     /gene="rhtC"
                     /locus_tag="ACHJ8C_001933"
     CDS             24118..24738
                     /gene="rhtC"
                     /locus_tag="ACHJ8C_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO5551794.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(24799..25419)
                     /gene="rhtB"
                     /locus_tag="ACHJ8C_001934"
     CDS             complement(24799..25419)
                     /gene="rhtB"
                     /locus_tag="ACHJ8C_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO5551795.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            25530..26552
                     /gene="pldB"
                     /locus_tag="ACHJ8C_001935"
     CDS             25530..26552
                     /gene="pldB"
                     /locus_tag="ACHJ8C_001935"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO5551796.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            26560..27360
                     /gene="yigL"
                     /locus_tag="ACHJ8C_001936"
     CDS             26560..27360
                     /gene="yigL"
                     /locus_tag="ACHJ8C_001936"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO5551797.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPEII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFNYALYEPGLLEPEGVSKVFFTCDSHEKLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLQDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            27436..28335
                     /gene="bioP"
                     /locus_tag="ACHJ8C_001937"
     CDS             27436..28335
                     /gene="bioP"
                     /locus_tag="ACHJ8C_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO5551798.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALNE"
     gene            complement(28223..29176)
                     /gene="metR"
                     /locus_tag="ACHJ8C_001938"
     CDS             complement(28223..29176)
                     /gene="metR"
                     /locus_tag="ACHJ8C_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO5551799.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALESFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            29294..31555
                     /gene="metE"
                     /locus_tag="ACHJ8C_001939"
     CDS             29294..31555
                     /gene="metE"
                     /locus_tag="ACHJ8C_001939"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO5551800.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
                     LLGPITYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
BASE COUNT         7334 a         8131 c         8472 g         7775 t
ORIGIN      
        1 aaatattcac tcaggaagtt attactcagg aagtaaagag gattacagaa ttatctcata
       61 acaagtgtta agggatgtta tttcccggtt ctctgtggca taataaaaga gtagatgctc
      121 attccatctc ttatgttcgc cttagtgcct cataaactcc ggaatgacgc agagccgttt
      181 acggtgctta tcgtccactg acagatgtcg cttatgcctc atcagacacc atggacacaa
      241 cgttgagtga agcacccact tgttgtcata cagacctgtt ttaacgcctg ctccgtaata
      301 agagcaggcg tttttttata tatcagaaag gccccggagg tgcttgcctc cgggtgagaa
      361 agagctactg tggcgggttg ttctgcaacg ttaacatcaa accgtcgcga cgcatcgctg
      421 cggcttcttc cggcttgtgc agtctgtcca gcgcgtcggc aagccatgcg taatcgtagg
      481 cgtccggacg ttgtttcagc gctgcgcgga aggcgagcga tgcttcctgc cattctccgt
      541 gtttcatcag cgactgaccc agtgtgctcc acaacagcgg gcgatcgccc acgtttttga
      601 tctgttggcg cagcactttt tccagctgtt ccgggttatt ggttttcaga cgcgggatcg
      661 gcagcagcag gcgatcgtca tactggcgtt tcaggccatc gatgataatt tgctgggcag
      721 tatcatgatc gtcacactca ataagatgtt ccgccattgc cacctgcaag gccacctgat
      781 gacgcgtttt ccggctttgg tttttccacc agttacgcaa accttcacta ccgttatcgg
      841 cacgcgcctg atccatcagg ccaatccatg cctgttgttc cagcattgca cgatgttctt
      901 catcaccaac atgggctttc gccattgatg ggatgatatc cagcagcgaa ctccatgcac
      961 cagtgcggat atacgcctgt tccgccagac gtaatacttc cggatggcgt ggcgtaactt
     1021 ccagcagctt atccacgccg tgacgtgctg catggttttc attacgggcc agttgcagac
     1081 gtacacgggt gatttctacc ggaatggtgt cattgccggc cagctccgct gcgcgttcca
     1141 gatgttggtt ggcgcgtgct tcatcaccac gttgttgcgc ggcttcggct gccagtagat
     1201 agttcaccac cggttgttca gcgtgatcgg catttttcgc catcagcttt tcaacttgct
     1261 gataatcgcc ttccgccagt ttcagcagcg cctgttcggt ctgcttacgt gcacggcgac
     1321 gcttacgtcc gacaaaccac ccacgggtgt gcgcgcctgt gcggaagatt cgccgcagta
     1381 gccactcaat ggcaaacagc actaccatcg ccagaatcaa aataatcgcg aggcccgtga
     1441 cgctggtttc aatattgtag ttgtcggtct ggatcagcac gtaaccctga tggccggcaa
     1501 tcatcgggcc aaccacgatc cccgcaatca gcaacacaaa gagcaataac acttttagca
     1561 tggttattct ccttgcggcg cggctgccgg agcatcagct tgcggtgcag gtgcaggtgc
     1621 aggtgcaggt gcaggtgcag gtttagcttc cgttgtccct gctgccggtt gtgccagcag
     1681 gttacgcacg cgggtctgca tcaatttttc cagcatcgcc tggctttgca gggtttccgg
     1741 aagatccatc gagatatttt gctggcttaa ctggtccacc tcgtcgagga acgctttggt
     1801 ggtggcatca tcagtatcgt agtaagcacg tacccaggtg gagacgttct ccagcgcctg
     1861 gcgataagtc tcttcctggt gacgcggtac agcttgtgct gcgaccagca ggcgagagcg
     1921 aatattttcg cgcaaataga tatcctgatt tggcgctaac agcggtacgg cggtgtcatc
     1981 acgacggcga atcgtaatga agttgtccat aaagttctgc cagctttttt gcagattgat
     2041 acgccattcg ctgatggaac tggaaagctc ttcaccgtca gaatccatcg gcgaaccatc
     2101 gctgtcatta tcggccagac gcagattatc tacctgattt gaaagctgat taagcttaag
     2161 gatgataccg tcataatcca cctgcgatac cgcagaaagg ctggcgatat catcggtaat
     2221 tgcccggcga acggtaatca gactcgggtc attcatatcc gccaggctgg cgtctgcact
     2281 tttcagcaac gctgcagcgg tcgtgacgtc ctgatcgctc cacagcttcc gtccggcgag
     2341 tttcaccaga aaatcggcct gggccagcag ccaggtttta gcatcgctgc cggaaatggt
     2401 ggcgaccttt tgttggactt catccagctg ttttgccagc gtttcttgct gacgattcgc
     2461 ctgctcaagc tgtacagctt gttgcttgat aatgccttcc agctcggctt tttggctctc
     2521 ctgggctttt tgcagtgccg taagttggtt agccaggaca tcgctggtgg cagtctgatt
     2581 gacggcctgt tgtttacccc agccatacaa accgacgccc gccgccagag caatagcgat
     2641 agccaccgcg ctgagaatca atgcggtatt gttcttactc tttttttctg ttgcgacagg
     2701 ttgtgacgtg gtgtccacgg cctccctggt ctcttcaacc acggcggagg ttttttcttg
     2761 ttccgtcatt atggcttcct gttatgagag ttattgtaat gcccgtaaaa gcgcatcgtt
     2821 gtcagcgtta tcggcgacct taatgtcttg ccagcccagt tcccgggcga gtttcgccaa
     2881 acgctcactg acgaccaata gtcgacagcg tagtaaccag tgctcacgat accattgtgg
     2941 gatcagcgac cagagttgct gcaacatttc accgctggta acaacgaccg tcgtcacctc
     3001 gcgggattgc cagcgcatcg cttcttctgc accatcgtaa tggattgcgc atcgttgata
     3061 acattcacaa aaagtgacct cagcaccgcg cgccgtcagg gaatccctaa ttagctcacg
     3121 accgccattg ccacgtaata tcagcgcacg tttgcccgca atattttgta attcaggtaa
     3181 ttgtagcaag acttcgctga tttcccgatc ctgcgggtag agaatcttct gtccgcttac
     3241 ggtatgtagt gccagcgcgg tggtgcgtcc aatggcgaaa taagtaggta gtcggggcca
     3301 tttacgatct tgctgatgca gctgtgattg ggcaaaagca accgcgtgtt gcgagagggc
     3361 aaacaacaga tcgctctctc ccagcgctgc cagttgatca gcaagttgcg gtaattgtcg
     3421 acccggagaa aactcaatca gcggaaaatg ccaggccacc tgccccagtg tgcgcagacg
     3481 gctcactaac tcttctcctg cgggagacgg gcgggtgacc aggatactca tgccggggcg
     3541 tctccgttat agacttcagc gaggatctcg cgcgcgccgt tattcagtag ctcttctgcc
     3601 agcgaaatcc ccatttgttc ggcatcttgc ggcgcaccgc ggcgttcacc gcgaataatc
     3661 tgcgaaccgt ccggcgcgcc aaccaacgca cgcagccaga tttcgccatc aataagctcg
     3721 gcgtagctac caattggcac ctgacatccg ccttcgagac gggtattcat ggcgcgttcg
     3781 gcggtaacgc gcagtgcagt ttcgtggtga ttcagcgcgg cgagcagctc gcgagtgcgt
     3841 gcatcatcaa ggcggcattc aatgcccacc gcaccttgtc ctaccgccgg aagagaaatc
     3901 tcgggtggca acgcggcgcg aatgcgggac tccagaccta aacgttttag tccggctacg
     3961 gcaagaatga tggcatcata ttcgccgtta tccagtttgc tcagacgagt gccgacgttg
     4021 ccgcgcaggg agcggataat cagatccggg cggcgttcag ccagttggca ctggcgacgt
     4081 aaactggacg tcccgacgat actgcctgcc ggtaacgcat ccagactgtc atagttattg
     4141 gacacaaatg catcgcgagg atcttcgcgc tcgcaaatgg tgaccagccc cagaccttgc
     4201 gggaattcaa cgggcacatc tttcattgaa tgaacggcaa tatcggcgcg attttccagg
     4261 agcgcgactt ccagctcttt cacaaataag cctttcccgc ctactttcgc cagcggcgta
     4321 tcaagaatca catcgccgcg cgtcaccatc ggtaccagtt caacgaccag gcccggatgg
     4381 ctcgccatca acttgtcttt gacatagtgt gcctgccaga gtgcaagtgg gctttggcgt
     4441 gtggcaattc ttaaaacatt gtctaacatg cttgttaccg tcattatcat ccgtggtcca
     4501 tcctaacatc cttgccagag cgatgtcagt gttgtggtga aacgtagacg cctgcgcaaa
     4561 ccgtaaaatg aggtctggca gtggatcctg acaggcgctt tgagccttcg taataaggaa
     4621 tttacagaga ataaacggtg ctacacttgt atgtagcgca tctttcttta cggtcaatca
     4681 gcaaggtgtt aaattgatca cgttttagac cattttttcg tcgtgaaact aaaaaaacca
     4741 ggcgcgaaaa gtggtaacgg ttacctttga catacgaaat atcccgaatg ccgcgtgtta
     4801 ccgttgatgt tggcggaatc acagtcatga cgggtagcaa atcaggcgat acgtcttgta
     4861 cctctatatt gagactctga aacagagact ggatgccata aatcaattgc gtgtggatcg
     4921 cgcgcttgct gctatggggc ctgcattcca acaggtctac agtctactgc cgacattgtt
     4981 gcactatcac catccgctaa tgccgggtta ccttgatggt aacgttccca aaggcatttg
     5041 cctttacacg cctgatgaaa ctcaacgcca ctacctgaac gagcttgaac tgtatcgtgg
     5101 aacgtcagta caggacccac cgaaaggtga gcttccaatt actggtgtat acaccatggg
     5161 cagcacctcg tccgtagggc aaagttgttc ctctgacctg gatatctggg tctgtcatca
     5221 atcctggctc gatagcgaag agcgccagtt gctacaacgt aaatgtagtc tgctggaaag
     5281 ctgggccgcc tcactgggtg tggaagtcag cttcttcctg attgatgaaa accgcttccg
     5341 tcataatgaa agcggcagcc tggggggcga agattgtggc tccacccagc atatactgct
     5401 gcttgacgaa ttttatcgta ccgccgtgcg tctcgccggt aagcgtattc tgtggaatat
     5461 ggtgccgtgc gacgaagaag agcattacga cgactatgtg atgacgcttt acgcgcaggg
     5521 cgtgctgacg ccaaatgaat ggctggatct tggtggctta agctcgcttt ctgctgaaga
     5581 gtactttggt gccagccttt ggcagctcta caagagtatc gattccccat acaaagcggt
     5641 gctgaaaaca ctgctgctgg aagcctattc ctgggaatac ccgaacccac gtctgctggc
     5701 gaaagatatc aaacagcgtt tgcacgacgg cgagattgta tcgtttggcc tcgatccata
     5761 ctgcatgatg ctggagcgtg ttactgaata tctgacggct attgaagatt tcacccgtct
     5821 ggatttagtt cgccgctgct tctatttaaa agtgtgtgaa aaacttagcc gtgaacgcgc
     5881 ctgcgtaggc tggcgtcgtg aagtgttaag ccagttggtg aaagagtggg aatgggacga
     5941 ttctcgtctg gcaatgcttg ataaccgcgc taactggaag atagatcagg tgcgtgaggc
     6001 gcacaacgag ctgctcgacg caatgatgca gagctaccgt aatctgatcc gctttgcgcg
     6061 tcgcaataac cttagcgtct ccgccagccc gcaggatatc ggcgtgctga cgcgtaaact
     6121 gtacgccgcg tttgaagcat taccgggtaa agtgacgctg gtaaacccgc agatttcacc
     6181 cgatctctcg gaaccgaatc tgacctttat ttatgtaccg ccgggccgcg ccaaccgttc
     6241 aggttggtat ctgtataacc gcgcgccaaa tattgagtca atcatcagcc atcagccgct
     6301 ggaatataat cgctacctga ataaactggt ggcgtgggca tggtttaacg gcctgctgac
     6361 ctctcgcacc cgtttgtata ttaaaggtaa cggcattgtc gatttgccta agttgcagga
     6421 gatggtcgca gacgtgtcgc accatttccc gctgcgctta cctgcaccga caccgaaggc
     6481 gctctacagc ccgtgtgaga tccgccatct ggcgattatc gttaacctgg aatatgaccc
     6541 gacggcagcc ttccgcaatc aggtggtgca ttttgatttc cgtaagctgg atgtcttcag
     6601 ctttggcgag aatcaaaatt gcctggtggg tagcgttgac ctgctgtatc gcaactcgtg
     6661 gaacgaagtg cgtacgctgc acttcaacgg tgagcagtcg atgattgaag ccctgaaaac
     6721 cattctcggc aaaatgcatc aggacgccgc accgccagat agcgtggaag tcttctgcta
     6781 cagccagcat ctgcgcggct taatccgtac tcgcgtgcag caactggttt ctgagtgtat
     6841 tgaactgcgt ctttccagca cccgccagga aaccgggcgc ttcaaggcgc tgcgcgtctc
     6901 cggtcaaacc tggggcttgt tcttcgaacg gctgaatgta tcggtacaga aactggaaaa
     6961 cgccatcgag ttttatggcg ctatttcgca taacaaactg cacggcctgt cagtgcaggt
     7021 tgaaaccaat cacgtcaaat taccggcggt ggtggacggc tttgccagcg aagggatcat
     7081 ccagttcttc ttcgaagaaa cgcaagacga gaatggcttt aatatctaca ttctcgacga
     7141 aagcaaccgg gttgaggtgt atcaccactg cgaaggcagc aaagaggaat tggtgcgtga
     7201 cgtcagtcgc ttctactcgt cttcgcacga ccgtttcacc tacggctcaa gctttatcaa
     7261 ctttaacttg ccgcagttct atcagattgt gaaggttgat ggtcgtgaac aggtgattcc
     7321 gtttcgcacc aaatctatcg gtaacatgcc acctgccaat caggagcatg acgcgccgct
     7381 gttgcagcag tatttttctt gatgctaatg ccggaaagcg aggcttatcc ggcattagct
     7441 cttaacgaaa actgacggtt tctcccgcct gctgcgtcgc ggcctgttcc aataaatccc
     7501 agaaggtttc gtcgctgcga tcgcaaatcc actcatcgcc tttcagatca aaatggtagc
     7561 cgccctgttt ggttgccagc catacctggt gcagtggttc ctggcggttg ataatgattt
     7621 tgctgccgtt ctcaaaggta atggttagta caccgccgtt gatttcacag tcgatatcgc
     7681 tgtcgccatc ccagtcgtcc agatgttctt caatggtcag ccacagttga tcagccaggc
     7741 gatgaaattc actgtcgttc attgttgtat cctgttttta agtgatggcg gcagtatagc
     7801 ggcatggggt cagggcttca aagtttgctc gtctgctgcg ttctggcatg attcatccgt
     7861 caacggaata ataatgtctc tgttgtgaag ttcatgcaca ttctcttctc tgacgcgtgt
     7921 tttcgccggg cagggggagg gcgtaatcct tacctctgta tcggtccatg tataaacatt
     7981 gcgatcgtca taagcaaagc gatcgttttt agttagcggg ccgttgttag ttggaataat
     8041 ttgcacgctc ttgcgtgacc gtaattggtg gataaagtaa gcatgctgtt tgccgcgatt
     8101 aaatgagtga tcaatgacca ggtctggcga atccagcggg attatttcaa gctggtcttc
     8161 accaaaagtc gataattttg ttacatacag ccgatcttta tactgcgcaa tattgtcagt
     8221 aatcagatga aattgttcag gttcaatacc ttgtaggtct atccactggc tccactgacg
     8281 gtcgggcgtg agctgtttac gccagcgaac gcgactttcc cctacttcaa aagagacgtc
     8341 atcatctacg ggtatcgctt tgccagataa ttgaccaaag ggataacgat gcagaccctt
     8401 attgtcgcga aaaaccagca gtgaatgagg tatccagcga attatctcaa atgagtctgc
     8461 attcgccgta aggtgagcgc catttaaata aatattctgc ccactgcgga taagcagatc
     8521 attggaatac aaatttctgt ttacactcat aagacgtgga ttaatacccc accatctttt
     8581 tttctaatac agtaacatta ctgctgctcc cctgacgtcg accggacaag tagagatttt
     8641 tactatccac cagcgtggta gagttacctt cgatagcttt tagtgtcgcc agatcaacac
     8701 gtgacgcgcc gctattactt tcggtacgct gtccgtcaaa atagaggcta tatttatcga
     8761 ccgcgaagcg gcccatagcc tgaaagctgg caatatcgac agtgggtgtt cccggtggat
     8821 tgctaacgat tttgccacgc cagagaataa cgcggccgtc tgtcacccaa tggtattcct
     8881 cattttttat gcttatttca tcggtgggta tatagccttc caccaactcc cgctccccgg
     8941 atatatttgt cacaaggcgc aaatcggctc tgttcatctt cggaaattgg gcaatgattt
     9001 tctcatcctc cccataacct ttaccgtaag acataacacc gtgatcattg taatagtcga
     9061 accctatggc ctgtacagca ggcgtcagaa atatgtgccc caacaggatg agtgcaacag
     9121 tgattatttg accattcatt ttgttgcctc attgcgtggg gcaggaataa attcgccgtc
     9181 atcggtaatt tttccgtcca gatatcccgt tttgtaattt acgccatagc catatcccga
     9241 atgttgttcc ttccagatta agtgaacttg tttcggatca gcggtgcggt atcgccagag
     9301 ctgcgcgcca tcaaagctgt aaacgtagcg gttatcgcgg gcataccatc tgcgcaaaaa
     9361 gggatggtca ttaagatgtg cttcgcgttc atccggagag cggttatcca gtaagatcag
     9421 cggtccgtca gattcaaaca cgtgtaaact ggaatggttt acccaccctt cggcacgcgt
     9481 aagtaaatag ccgtggcgtt tcccggcgtt gaaacgtttg ttgataataa gattggggtc
     9541 atcgagtttt acgatctcca acttgcggtc accagactct gtttcgatga tggtataaag
     9601 cgaatcctga tactgggcga cagcatcact gatggggtgg aactgttcag gatcgagtcc
     9661 cggtaattct tcctgtttac agtcaggccc ctgacgccag aacactttct tttccagcag
     9721 agaaaaatcc agacagtgct ttgttagatc ctcatcccac gccagatttt ccttgtcgag
     9781 gactttacgc tgtaacccgt ttttatcacg ccaggtcaag agtgagccgg gcatccagcg
     9841 cacgacggaa aaggtgtccg ggtcggcatc cagttgttct ccgttgatca tgattttcgt
     9901 ctgtgtgcgg gctagcatat cccaggggcc aaatggggca agaaggcagg gattaaatgc
     9961 tgtagagaat ttaccgcgct gatcccatga cttttgagcc agtaccgtaa aggtgtcggg
    10021 atcatcaagg cgctgaccat ttgcgtaaag atagtgcttg tcacggagaa tcaaacctag
    10081 ccagtctggt ttccattgcg ggttaaattc tacctgacgc aacgttcggc ggtttagttg
    10141 ctggttgcta tcggtgcgtt tgccttcgta atagaggctg tacttatctg cggcaaaatc
    10201 accccatgcc tgaaatgacg agatatcgac tggcggtgtg cctggagggt tttgcacaat
    10261 ctcgccagcg tagagaatag cgcgaccatc gctatagtaa gagtaatggg gaagagttgc
    10321 gtttgcgccg ctgtagacaa cgtctgtcat gagattggta gctgggggca ccgtttccca
    10381 atcctccagt ttggttgggt caattagcaa attcggtaac agacgcagac gcttgtagtt
    10441 aatctgcggt agcgaaagat tggtcttttc ataagggcgc aggtacacca cgccttttcc
    10501 ctgtttctgg taaatattat gttccggctc atcaagccga catccccatg ccgacgataa
    10561 aaacaacgaa caagacaaca aacaaaccac attgcgatag tgcataaagc catcctggcg
    10621 cgaggtgccg atcacgaaac taccagcaaa acataaatcg ccacgagtaa gcgttatact
    10681 cgcagcattt cctcactttt cagacttcat aaagagtcgc taaacgcttg cttttacgcc
    10741 ttctcctgcg atgatagaaa gcagaaagcg atgaacttta caggcaatcc ataatgaaaa
    10801 acgtgtttaa ggcactcact gtattactta ctctcttcag cctgacgggc tgcggtctga
    10861 aaggtccgct ctatttcccg cctgcagata aaaacgcacc gccgccgacc aaaccggtag
    10921 agacgcaaac gcaatccacg gtgccggata aaaacgaccg cgccactggc gatggtccat
    10981 cccaggtgaa ttactaaaag tcagtttctg tacccgcgtg attggagtaa atgatgcagt
    11041 tctcgaaaat gcatggcctt ggcaacgatt ttatggtcgt cgacgcggta acgcagaatg
    11101 tctttttttc accggagctg attcgtcgcc tggcggatcg gcacctgggg gtagggtttg
    11161 accaattgct ggtggttgag ccgccgtatg atcctgaact ggattttcac tatcgcattt
    11221 tcaatgctga tggcagtgaa gtggcgcagt gcggcaacgg cgcgcgctgc tttgcccgtt
    11281 ttgtgcgcct gaaagggctg accaataagc gcgatatccg cgtcagcacc gccaacgggc
    11341 ggatggttct gaccgtcacc gatgatgatc tggtccgcgt aaatatgggc gaacccaact
    11401 tcgaaccttc cgccgtgccg tttcgcgcta acaaagcgga aaagacctat attatgcgcg
    11461 ccgccgagca gacaatctta tgcggcgtgg tgtcgatggg aaatccgcat tgcgtgattc
    11521 aggtcgatga tgtcgatacc gcggcggtag aaacgcttgg tcctgttctg gaaagccacg
    11581 agcgttttcc ggagcgcgcc aatatcggtt ttatgcaagt ggttaagcgc gagcatattc
    11641 gtttacgcgt ttatgagcgt ggggcaggag aaacccaggc ctgcggcagc ggcgcgtgtg
    11701 cggcggtggc ggtagggatt cagcaaggtt tgctggccga agaagtacgc gtggaactcc
    11761 ctggcggtcg tcttgatatc gcctggaaag gtccgggtca cccgttatat atgactggcc
    11821 cggcggtaca tgtctacgac ggatttattc atctatgaag caaccagggg aagaactgca
    11881 ggaaacactc acggagcttg atgaccgggc ggttgtcgat tatctgatta aaaatcctga
    11941 gttttttatc cgcaatgcgc gcgcagtaga agcgatacgt gtgccgcatc cggtacgcgg
    12001 caccgtttcg ttggtcgagt ggcacatggc ccgcgcacgt aatcatattc atgttctgga
    12061 agagaacatg gcgttgttga tggaacaggc tatcgccaac gaaggcctgt tttatcgtct
    12121 actctacctg cagcgcagtc tcaccgccgc cagcagtctc gatgatatgc tgatgcgctt
    12181 tcaccgctgg gcgcgcgatc tcggcctggc aggtgcgagt ctgcgcctgt ttccagatcg
    12241 ctggcgttta ggtgcgccgt cgaaccacac tcatctggca ttaagccgtc agtctttcga
    12301 accgctgcgt attcagcgtc tggggcagga acagcactat ctggggccgc ttaacggacc
    12361 ggagcttctg gtggtgctac cggaagcgaa agcggtggga tcggtggcga tgtcgatgct
    12421 gggaagcgat gctgatttgg gtgttgtgct gtttaccagt cgcgatgcca gtcactatca
    12481 acaagggcag ggaacgcagt tacttcatga aattgcactg atgttgccgg agcttctggc
    12541 gcgttggatt gaacgcgtat gaccgattta cacaccgatg tagaacgcta cctacgttat
    12601 ctgagcgtgg agcgccagct tagcccgata accctgctta actaccagcg acagcttgag
    12661 gcgatcatca attttgccag cgaaaatggc ctgcaaagct ggcagcaatg tgatgtgacg
    12721 atggtgcgta attttgctgt acgcagtcgc cgtaaagggc tgggagctgc aagtctggcg
    12781 ttacggcttt ctgcgctacg tagctttttt gactggctgg tcagccagaa cgaactcaaa
    12841 gccaacccgg caaaaggtgt ttcggcaccg aaagcgccgc gtcatctgcc gaaaaatatc
    12901 gacgtcgatg atatgaatcg actgttggat attgatatca acgatcccct cgccgtgcgc
    12961 gatcgggcaa tgctggaagt gatgtacggc gcgggtctgc gtctttccga actggtgggg
    13021 ctcgatatca aacacctcga cctggagtcc ggcgaagtgt gggtgatggg gaaaggcagc
    13081 aaagagcgcc gcctgccgat tggtcgcaac gctgtggcgt ggattgagca ctggcttgat
    13141 ttgcgcaacc tgtttggaag cgaagacgat gcgcttttcc tgtcgaaact gggcaagcgt
    13201 atctctgcgc gtaatgtgca gaaacgcttt gccgaatggg gcataaaaca agggctgaat
    13261 aatcacgttc acccgcataa attacgtcac tcgttcgcta cgcatatgct ggagtcgagc
    13321 ggtgatcttc gtggtgtaca ggagctgctg ggtcatgcca acctttctac cacgcaaatc
    13381 tatactcatc ttgattttca acaccttgcc tcggtgtacg atgcggcgca tccacgcgcc
    13441 aaacggggga aataatgcgt ttttaccggc ctttggggcg catctcggcg ctcacctttg
    13501 acctggatga taccctttac gataaccgtc cggtgatttt gcgcactgag caggaggcgc
    13561 ttacctttgt gcaaaattac catccggcgc tgcgcagctt ccagaatgaa gatctgcaac
    13621 gtctgcgtca ggcggtacgg gaagcggaac ccgagattta tcatgatgtg acgcgctggc
    13681 gttttcgctc aatagaacaa gcgatgctcg acgccgggct gagcgcagaa gaagccagtg
    13741 caggcgcaca cgcggcaatg atcaactttg ccaaatggcg tagccggatc gacgttccgc
    13801 agcaaacgca cgacacctta aaacagctgg cgaagaaatg gccgctggtg gcgatcacca
    13861 acggtaacgc ccagccggag ctgtttggtt tgggggatta ttttgagttt gtgctgcgtg
    13921 ctggcccgca cgggcgctcg aaaccgttca gcgatatgta ctttctggcg gcggaaaaac
    13981 tcaacgtgcc gatcggcgag atcttacatg ttggggacga tctcaccact gacgtgggtg
    14041 gggcaattcg cagcggaatg caggcctgtt ggatcagacc ggaaaatggc gatctgatgc
    14101 aaacctggga cagccgttta ctgccgcatc tggaaatttc ccggttggca tctctgacct
    14161 cgctgatata atcagcaaat ctgtatatat acccagcttt ttggcggagg gcgtcgcgct
    14221 tttccgccca acctattttt acgcggcggt gccaatggac gtttcttacc tgctcgacag
    14281 ccttaatgac aaacagcgcg aagcggtggc cgcgccacgc agcaaccttc tggtgctggc
    14341 gggcgcgggc agtggtaaga cgcgcgtact ggtgcatcgt atcgcctggt tgatgagcgt
    14401 ggaaaactgc tcgccgtatt cgattatggc ggtgacattt accaacaaag cggcggcgga
    14461 aatgcgccat cgtatcgggc aactgatggg taccagccag ggcggtatgt gggtcggcac
    14521 tttccacggg ctggcacacc gtctgctgcg tgcgcaccat atggacgcca acctgccgca
    14581 ggatttccag atcctcgaca gcgaagacca gctgcgcctg cttaagcgtc tgatcaaagc
    14641 catgaacctc gacgagaagc agtggccgcc gcgccaggca atgtggtaca tcaacagcca
    14701 gaaagatgaa gggctgcgtc cgcatcatat tcaaagctac ggtaatccgg tggagcagac
    14761 ctggcagaag gtgtatcagg cgtatcagga agcgtgtgac cgcgcgggct tggtggactt
    14821 cgcggagctg cttttacgtg ctcacgagtt gtggcttaac aagccgcata tcctgcaaca
    14881 ctaccgcgaa cgttttacta acatcctggt ggacgaattc caggatacca acaacattca
    14941 gtatgcgtgg atccgcctgc tggcgggcga tactggcaaa gtgatgatcg ttggtgatga
    15001 cgaccagtca atctacggct ggcgcggggc gcaggtggag aatattcagc gtttccttaa
    15061 tgatttcccc ggtgccgaaa ccattcgtct ggagcagaac taccgctcca ctagcaacat
    15121 tctgagcgcc gctaacgccc tgattgaaaa caacaacggg cgtttgggta aaaagttgtg
    15181 gaccgatggc gcggacggtg agccgatttc cctctattgc gcttttaacg aactcgacga
    15241 agcgcgtttt gtggttaacc gtatcaagac ctggcaggat aacggcgggg cgctcgccga
    15301 gtgcgccatt ctctatcgca gcaacgccca gtcgcgcgtg ctggaagaag cactgttgca
    15361 ggcgagtatg ccgtaccgta tttacggcgg gatgcgcttc ttcgaacgcc aggaaatcaa
    15421 agatgcgctc tcgtatttac gcctgattgc caaccgcaac gacgacgcgg cgtttgagcg
    15481 tgtggtgaat acgccaacgc ggggtattgg tgaccggacg ctggacgtgg tacgtcagac
    15541 atcgcgagat cgccagttaa cactctggca ggcatgtcgt gaactgttgc aggaaaaagc
    15601 cctcgccgga cgtgctgcca gcgccttaca gcggtttatg gagctgatcg acgccttagc
    15661 gcaggaaact gccgatatgc cgctgcatgt acagactgac cgggtaatta aagactccgg
    15721 cctgcgcacc atgtacgagc aggagaaggg cgaaaaaggt cagacgcgta tcgaaaactt
    15781 agaggaactg gtgacggcaa cgcgccagtt cagctacaac gaagaagacg aagatttaat
    15841 gccgctgcag gcattcctct cccatgcggc gctggaagcg ggcgaggggc aggcggatac
    15901 ctggcaggat gcggtgcagt tgatgacgct acactcggcg aaaggtctgg aattcccgca
    15961 ggtgtttatc gttggtatgg aagagggcat gttcccaagc cagatgtcgc tggatgaagg
    16021 cgggcgtctg gaagaagaac gccgtctggc ctacgttggc gtaacccgcg cgatgcagaa
    16081 actgacgctg acctacgcgg aaacccgccg tctgtatggc aaagaggttt accatcgccc
    16141 gtcgcgcttt atcggtgagt tgccggaaga gtgtgtggaa gaggtgcgcc tgcgcgccac
    16201 ggtaagccgc ccggtcagcc atcagcgtat gggtacgccg atggtcgaga acgacagcgg
    16261 ctataagctc ggccagcgcg tacgccacgc taagtttggt gaaggcacca tcgtcaatat
    16321 ggaaggcagc ggcgagcata gccgtttgca ggtggcattc cagggccagg gtattaaatg
    16381 gctggtggcg gcttacgccc ggttggagac ggtgtgacgt tgcggaatgc ggtgcgccat
    16441 accgcattcc gcatcagcac cttacttagc gcacttcttc acatcaatat tccagtcagc
    16501 accagatgca acctgatgtt tctgcgcgaa attatccata ttcagcgcca cggaaacatt
    16561 caataagctg ttcagataca ccatcggctg gccttccggc acatcgccga agcttgcgac
    16621 atacggcatt ttccctgcgt atttttgctg ggaaccttcg gaaatggtca cacacaacgt
    16681 gtcgttgagt ttgattcctg cctgattcag caactcgtcg ctgatattgc tccagacgtt
    16741 gccgtactga atatcgagaa tcgggatatt gcccttcact tcaccttttg ttgctttggc
    16801 tttctgataa gaaagttcga caacttttgc aggaagctct ggccccacct gctcgaacgt
    16861 gatcgccccg gaagccagac gtgcaccggt gtaggcatac acgtcacgac cgtggaaggt
    16921 atatgacttt tcggaacctt tcagacggtt ggttttttca tcgatttgcc ggacgctctc
    16981 aatacccagc gattccgcca ccaacgtgag cgtaccgtta tctggtgaaa cgaagtactg
    17041 accgtttttg gttttcagca ctaccgattt acggtctgta ccaacaccag gatcgaccac
    17101 gctaacaaac accgaacctt gcggccagta actggcagtc tgatacaggc ggtaagcgcc
    17161 ttcccagatg ttatacggtg gaatttcgtg tgttaaatca aatattttaa gattatggtc
    17221 aacgccgaaa gcgacccctt tcatcgccga gacggcgcca tctttcaggc taaaatccgt
    17281 ttgtaaaatc aaagcgttat ccgcataggc ggatgatgat cccagacacg ttaataaaaa
    17341 taacgttgcg agcgtttttt tcatttttgt ttcccttcat ttatctgatg attttccagc
    17401 tgtgtagcca gtcccagttt gtctggcctc ctcgagtgta caatgcttaa cctttccgct
    17461 cgacggaaat catggtgaca aaaggatagc gttgccacgg gattgccccg cctttcatat
    17521 acatatgtga aattgtgcca tcaagataaa gcaactgctc aacgttcagt ttcgctttgg
    17581 cataacaggc aaaatcataa aaatttgtcg cctgctggct caacaaaaac acggcgttcc
    17641 cttgtttatt aatcccaaca ccgttacgaa ttttacgtga ggcgacgttg ggatgaatac
    17701 gcggattaat tacgccgttt tccatcaaca ttggccctga ctgcaccgca aactgaatct
    17761 ctttactggt tttaaaggca tccagacgaa cgatgccgac tttatctccc gcgacataaa
    17821 acacgccgcc aggacgaata aagaaattcc cttcacctga agcgagattt aacgccacct
    17881 tctgctgacc gttttcgatg tacaaaccga gcggcgcata gctttcatca tagatgccgc
    17941 cgttcatcgc catctgcacc tgaccctgac tattaatatc cgccagcaga gcatgtaacg
    18001 ttccccacgc ttcgccattg gctttttgcc agtacatttt cacccgctct gtttgaggat
    18061 taacggtata cgcctgtacg gtcagcgtcg gatctgaggg tgcgcaatca tcagcggcaa
    18121 cagcaaacaa gggaagtaag gtgagggtga gaaaaatccg tttgagattc aaggtgatca
    18181 ttcctttacc aatgaatagc cgatgcgcca ttataggccc tggatgtggg atttttttat
    18241 cctgttagcg accttgacga gtaccaaaaa gcgcgaagtt caactattgt tctgtggtgt
    18301 tctgttgcgt gttgacggca aaattttgct ggcgtaacat gcgcgcacga tcactctaag
    18361 aggacattcg ccttggacac acccagtaga ttctggctca ctatcctgtc atccaggatc
    18421 aactcctaag gctatccctt tttgctgata gccttagcgg ttgtcagcga cctcaatttt
    18481 tcccgtcgcg ctgagtcagg ctgtttaatg gtctgaaacc caatttgttt ctgtgtgccc
    18541 accgaactgt ccgatatttt aagcattggg agtcccggtc atgctgagcg catttcaact
    18601 ggaaaataac cgactgaccc ggctggaagt cgaagagtca caaccccttg taaatgcagt
    18661 atggattgat cttgtcgaac cggacgacga cgagcgactg cgcgtacaat ctgaacttgg
    18721 ccagagcctg gcaacccgcc cggaactgga agacatcgaa gcatcggcac gtttctttga
    18781 agacgatgac ggcctgcata ttcactcctt cttcttcttt gaagatgcgg aagatcacgc
    18841 tggtaactcc actgtggcat ttaccatccg tgatggtcgt ctgtttactc tgcgtgagcg
    18901 tgaactgccc gcttttcgtc tgtatcgtat gcgtgcccgt agccagtcaa tggtggacgg
    18961 taacgcctac gagctgctgc tggatctgtt cgaaaccaaa atcgaacagt tggcagatga
    19021 aattgaaaat atctacagcg acctggagca gttgagccgg gtaattatgg aagggcatca
    19081 gggcgatgag tacgacgagg cgctctccac gctggcggaa ctggaagata tcggctggaa
    19141 agttcgcctg tgtctgatgg atacccagcg cgcgctcaac ttcctggtac gtaaagcacg
    19201 cttaccgggt gggcaactgg agcaggcgcg tgaaatcctg cgagatatcg aatccctgct
    19261 gccgcataac gaatccctgt tccagaaggt gaacttcctg atgcaggcgg cgatgggctt
    19321 tatcaatatc gagcagaacc gcatcatcaa aatcttctcg gtggtatccg tggtgttcct
    19381 gccaccgacg ctggtggctt ccagctacgg gatgaacttt gagtttatgc cagaactgaa
    19441 gtggagcttc ggctacccag gtgcgattat ctttatgatc ctcgcgggcc tggcaccgta
    19501 tctgtacttt aagcggaaga actggttgta actttacgat gtcgctgtga gtaagttagt
    19561 gtgctgacag cgacatttta gccgagggta tcgcagataa catacaaaga tagataagtt
    19621 agtgggtagg ctctttaaac ggcctaccca tgttaatgct taactttttc tacgctgcgt
    19681 ataaatcgca tccatcacaa aaattgccag cgccacccaa ataaaggcga aagttaccat
    19741 cttatcggca cccggttttt caccataaaa cgtcacagcc agcaggaaca tcagcgtcgg
    19801 accaatgtac tggaaaaagc ctaacgttga gagacgcagg cgtgtcgccg ctgcagtaaa
    19861 acacaacagt ggcacggtgg tgacaatacc ggcggcgatc agcagtaaat tcagcgacat
    19921 cgggttttgc cccatatgac tggttgagct gtcggcaata gcaaacaggt aaatcgccgc
    19981 cacgggcagc aaccacatag tttcgattaa catgccggtt tgcgcttcaa cagcaatctt
    20041 cttgcgcacc aggccgtaga aagcaaaact aaacgccagc cccagcgcga tgatcggcag
    20101 tgaaccaaaa gtccacaact gaaccatcac tccacatatc gccagaatca ccgccagcca
    20161 ttgcatccgg cggaatcgct cgccgaggaa aatcatcccc agcacaatgt tcaccagcgg
    20221 gttaataaag taaccaaggc tcgcttccag catatggtga ttgttcaccg cccagataaa
    20281 cagtagccag ttgccgccaa tcagcacggc agagactgcc agcataaaaa ttttctgtgg
    20341 cgtctgaatc agcgttttta aataggacca ctggcggcaa atgctcatca gcaccaccat
    20401 aaagaagaac gaccagatca cgcgatgcgt caggatctca tcggcgggca cgtagtaaat
    20461 cagtttgaag tacgctggcg caatacccca aataaaataa gcggcaagag cgagtaatac
    20521 gccctgccgc gtttgttttg catccatcgg gaatactcat ttttaattgg taacagcagt
    20581 ttacctgctt ttatgtcttc aacctaccat ataggtggcg gtggcactgg caatataaag
    20641 ctgttcttca ttgtgtaatt caacgcgggc gacggcgact ttattgcctg cacgcaacag
    20701 gctactggta gcagtaaaac gctcgcctct gcctgggcgc agataatcga cgcgaagatc
    20761 aatggtcccc atccgcgata gccgctggcg tagttcatct tcactgatgg tttcgtggcg
    20821 ggttaaggta cttcccacgc acaccagacc tgcggcgaca tccagcgccg acgcaatgac
    20881 cccgccgtgt aaaatgcttt gcgcccagtt gcccaccatc atcggctgat ttttaaaggc
    20941 cagctgtgcg aactcttttt cgtaacgctc cagttccatc cccaatgcgc ggttaaatgg
    21001 catgtgataa acaaacatct cacccactaa tttgagggct tgttcagcgg tcagtacggc
    21061 agacataaca tccttacact tcattagtta atgaaatgtt gattttatgc ttatttattg
    21121 ttggtttcta ctttaggaag ggataactaa cggctatgga gttaagtatg taaaatagcc
    21181 cgcagaaaaa tattcacctt atcaataatt cgttacggag aacacgagcg atgcggactc
    21241 tgcagggctg gttgttgccg gtgtttatgt tgcctatggc agtatatgca caagaggcaa
    21301 cggtgaaaga ggtgcatgac gcgccagcgg tgcgtggcag tattatcgcc aatatgctgc
    21361 aggagcatga caatccgttc accctttatc cttatgacac caactacatc atctacaccc
    21421 aaaccagcga tctgaataaa gaagcgattg ccagttacga ctgggcggaa aacgcgcgta
    21481 aggatgaagt aaagttccag ttgagcctgg cgtttccgct gtggcgtggg attttggggc
    21541 caaactctgt actgggcgcc tcctacacac agaaatcctg gtggcagctt tctaatagcg
    21601 atgagtcatc accgtttcgt gaaactaact acgaaccgca attgttcctc ggttttgcca
    21661 cggattacaa ctttgctggc tggacgctgc gcgatgtgga aatgggctac aaccacgact
    21721 caaacgggcg ttcagacccg acttcccgca gctggaaccg cctttatacc cgcctgatgg
    21781 cagaaaacgg taactggctg gtggaggtga agccgtggta tgtggtgggg aatacggacg
    21841 ataacccgga catcaccaaa tatatgggtt actaccagct taaaatcggc tatcacctcg
    21901 gtgatgcggt actgagcgcg aaagggcagt acaactggaa caccggttac ggcggcgcgg
    21961 agttaggctt aagctacccg atcaccaaac atgtgcgcct ttatactcag gtttacagcg
    22021 gttacggcga atcgctcatc gactataact tcaaccagac ccgcgttggt gtgggagtta
    22081 tgctaaacga cctgttttga tgaatggttg gcaaatctgg aatccagcat ccaggattac
    22141 cctctcagag actaaaagca ttgcagtttc tcgcgcaggc gctgaaaata gcgcctgttt
    22201 ttatttcagg caatcggggt taatgtggcg caggcggaag tgttgaatct ggagtccggg
    22261 gctaaacagg ttttacaaga aacctttggc taccaacagt ttcgccccgg ccaggaagag
    22321 attatcgaca ccgtactttc cggtcgcgat tgcctggtcg tcatgcccac tggtggcgga
    22381 aaatcccttt gctatcaaat ccctgcctta ttgctaaacg gccttaccgt ggttgtctca
    22441 ccgctgattt cgttgatgaa agatcaggta gatcaactgc aagccaacgg cgtggcggcg
    22501 gcgtgcctta actcgacgca aacccgcgag cagcaacttg aagtgatgac aggctgtcgc
    22561 accgggcaaa tccgcctgct ttatatcgcg ccggaacgcc tgatgctgga taactttctt
    22621 gagcatctgg cgcactggaa tccggtgtta ttagccgttg atgaagcgca ctgtatctcc
    22681 caatggggtc acgatttccg cccggaatac gctgccctcg gtcagctgcg tcagcgtttt
    22741 ccgacgctgc cgtttatggc gctgactgcc acagccgatg acaccacgcg ccaggatatc
    22801 gtgcgcctgc tggggctgaa cgatccgctg attcaaatca gcagttttga ccgtccgaat
    22861 attcgctaca tgctgatgga gaaattcaaa ccgctcgatc agttgatgcg ttatgtacag
    22921 gaacaacgcg gtaagtcggg gattatttac tgtaatagtc gtgcgaaagt agaagacacc
    22981 gctgcgcgcc tgcaaagcaa gggaattagc gcggcggcct atcatgccgg gctggaaaat
    23041 aatgttcgcg ctgatgtgca ggaaaaattc cagcgcgatg acctgcaaat tgtggtggcg
    23101 acggtagcgt tcggcatggg catcaacaag cctaacgttc gcttcgtggt gcattttgat
    23161 attccgcgca atatcgaatc ctattatcag gaaactggac gcgccgggcg tgatggcctg
    23221 cccgcggaag cgatgctgtt ttacgatccg gctgatatgg cgtggctgcg ccgttgtctg
    23281 gaagagaagc cgcaggggca gttgcaggat atcgaacgtc ataaactcaa tgcgatgggc
    23341 gcgtttgctg aagcgcaaac ttgccgtcgt ttggtattgc tgaactattt tggcgaaggg
    23401 cgtcaggagc tgtgcgggaa ctgcgatatc tgcctcgatc cgccgaaaca gtacgacggt
    23461 tcaaccgatg cgcagattgc cctttccacc attggtcgtg tgaatcagcg gtttgggatg
    23521 ggttatgtgg tggaagtgat tcgcggtgct aataatcagc gtatccgcga ctatggtcat
    23581 gacaaactga aagtctatgg catgggccgt gataaaagcc atgaacattg ggtgagcgtg
    23641 atccgccagc tgattcacct cggcctggtg acgcaaaata ttgcccagca ttctgcccta
    23701 caactgacag aggctgcgcg tccggtgctg cgcggcgaat cctctttgca acttgccgtg
    23761 ccgcgtatcg tggcgctcaa accgaaagcg atgcagaaat cgttcggcgg caactatgat
    23821 cgcaaactgt tcgccaaatt acgcaaactg cgtaaatcga ttgccgatga aagcaatgtc
    23881 ccgccgtacg tggtgtttaa cgacgctacc ttgattgaga tggcggagca gatgccgatc
    23941 accgccagcg aaatgctcag cgttaacggc gttggtatgc gcaagctgga acgctttggc
    24001 aaaccgttta tggcgctgat tcgtgcgcat gtcgatggtg acgacgaaga gtagtcagca
    24061 gcataaaaaa gtgccaggat gaagactccg taaacgtttc ccccgcgagt ctaatgtatg
    24121 ttgatgttat tcctcaccgt cgccatggtg cacattgtgg cgcttatgag ccccggtccc
    24181 gatttctttt ttgtctctca gaccgctgtt agtcgttccc gtaaagaagc gatgatgggc
    24241 gtgctgggca ttacctgcgg cgtaatggtt tgggctggga ttgcgttgct tggcctacat
    24301 ttgattatcg aaaaaatggc ctggctgcat acgctgatta tggtgggcgg tggcctgtat
    24361 ctctgctgga tgggttacca gatgctacgt ggtgcactga aaaaagaggt tgtttctgcc
    24421 cctgcgccac aagttgaact ggcgaaaagc gggcgcagtt ttcttaaggg cttactgacc
    24481 aatctcgcga accctaaagc gattatctac tttggttcgg tgttctcgct gtttgtcggt
    24541 gataatgtcg gcaccaccga acgctggggc attttcgcgt tgattattgt cgaaacactg
    24601 gcgtggttta ccgtcgttgc cagcctgttt gctctgccgc aaatgcgccg tggttatcaa
    24661 cgtctggcga agtggattga tggttttgcc ggggcgttat ttgccggatt tggcattcat
    24721 ttgattattt cgcgttaatg ccagacgcgt catcagagta agtcggataa ggcgtttacg
    24781 ccgcatccga cattattttc acgcatgcct cgccgatgct aacagcgctc ccaccagcat
    24841 aaataacgag ccgaaaatct tattcagcgc cttcatctgc tttggtcctt taatccatag
    24901 agcaatccgt tgagcaaggg tggcgtaacc gatcatcaca ataatatcga ccacaatagt
    24961 ggtgacgccg agcacgatat actgcatcag ttgtggctgc tgcggcatga tgaattgcgg
    25021 aaatagcgcc gccagaaaca caatactttt gggattggtg agattcacaa aaactgcgcg
    25081 ctggaacaaa tgtcgacgcg attgagtgga ggccagcgat ttaagatcaa ttgcgccagc
    25141 ggcgcgccat tgctgtattc ccagccaaat caagtaagct gcgcctgccc acttcaacac
    25201 ttcaaacgca atcactgagc gggaaaatag cgtccccaac cccacgccaa ccagcacaat
    25261 atgaatcgcc agtccggtct gaagcccagc aatagacgcc accgcgccgc gataaccgtg
    25321 gttgagcgag gtggtcatgg tgttgattgc gccagagcct ggcgacaggc ttaaaatgat
    25381 cgatgtcagc aggtaggcaa accaccattc taaggtcatg atgaactccc ggtgtgtcta
    25441 tttttgtgcc acaatacgct actgtcgcag cgttgtgtca ggcacgctta aaaaaacgat
    25501 tttacgtggt ttaagaggca gattacccga tgtttcagca gcaaaaagac tgggaaacaa
    25561 gagaaaacgc gtttgctgct tttaccatgg gaccgctgac tgatttctgg cgtcagcgtg
    25621 atgaagcaga gtttactggt gtggatgaca ttccggttcg ctttgtccgt tttcgcgcac
    25681 agcaccatga ccgggtggta gtcatctgtc cggggcgtat tgagagctac gtaaaatatg
    25741 cggaactggc ctacgacctg ttccatttgg ggtttgatgt tttaatcatc gaccatcgcg
    25801 ggcagggacg ttccggtcgc ctgttagccg atccacatct cgggcatgtt aatcgcttta
    25861 atgattatgt tgatgatctg gcggcattct ggcaacagga ggttcagccc ggtccgtggc
    25921 gtaaacgcta tatactggca cattcgatgg gcggtgcgat ctccacatta tttctgcaac
    25981 gccatccagg tgtatgtgac gccattgcgc taactgcgcc aatgtttggg attgtgattc
    26041 gtatgccgtc atttatggca cggcagatcc ttaactgggc cgaagcgcat ccacgtttcc
    26101 gtgatggcta tgcgataggc accgggcgct ggcgcgcgtt gccgtttgct atcaacgtac
    26161 tgacccacag cagacagcga tatcgacgta acttacgttt ctatgctgat gacccaacga
    26221 ttcgcgtcgg tgggccgacc taccattggg tacgcgaaag tattctggct ggcgaacagg
    26281 tgttagccgg tgcgggtgat gacgccacgc caacgcttct cttacaagct gaagaggaac
    26341 gcgtggtgga taaccgcatg cacgaccgtt tttgtgaact acgcaccgct gcgggccatc
    26401 ctgtcgaagg aggacggccg ttggtaatta aaggtgcgta ccatgagatc ctttttgaaa
    26461 aggacgcaat gcgctcagtc gcgctccacg ctatcgttga ttttttcaac aggcataact
    26521 cacccagcgg aaaccgctct acagaggttt aaatttctta tgtaccaggt tgttgcgtct
    26581 gatttagatg gcacgttact ttctcccgac catacgttat ccccttacgc caaagaaact
    26641 ctgaagctgc tcaccgcgcg cggcatcaac tttgtgtttg cgaccggtcg tcaccacgtt
    26701 gatgtggggc aaattcgcga taatctggag attaagtctt acatgattac ctccaatggc
    26761 gcgcgcgttc acgatctgga tggcaacctg atttttgctc ataacctgga tcgcgacatt
    26821 gccagtgacc tgtttggcgt ggtcaacgac aacccggaga tcattactaa cgtttatcgc
    26881 gatgatgaat ggtttatgaa tcgccatcgc ccggaagaga tgcgcttttt caaagaagcg
    26941 gtgttcaatt atgcgctgta tgagccggga ttgctggagc cggaaggcgt cagcaaagtg
    27001 ttcttcacct gcgattccca tgaaaaactt ctgccactgg agcaggcgat taacgctcgc
    27061 tggggcgatc gcgtcaatgt cagtttctct accttaacct gcctggaagt gatggcgggc
    27121 ggcgtttcaa aaggccatgc gctggaagcg gtggcgaaga aactgggcta cagcctgcaa
    27181 gattgtattg cctttggcga tggaatgaac gatgccgaaa tgctgtcgat ggcggggaaa
    27241 ggctgcatta tgggcagcgc gcatcaacgg ctgaaagatc tgcatccaga gctggaagtg
    27301 attggtacta acgccgaaga cgcagtgccg cattatctgc gtaagctcta tttatcgtaa
    27361 ttgttcttta tttggtcagt tgtcaacctg atacttcgct acaatggata ctcgttaatc
    27421 aaagagtttt ccattgtggc gctacttatc atcaccacga ttctgtgggc cttctccttt
    27481 agcttttatg gcgagtacct tgcggggcac gtcgatagct attttgcggt gctggtgcgc
    27541 gttggtctgg cggcactcgt ttttctgccg tttctgcgca cccgtggcaa tagcctgaaa
    27601 acggttggcc tgtatatgct ggtgggcgcg atgcagctcg gtgtgatgta tatgctgagt
    27661 ttccgcgcct atctctacct gacggtctcc gaactgttgc tgttcaccgt actgacgccg
    27721 ctctacatca cgctgattta tgacatcatg agtaagcgcc gtctgcgctg gggctatgct
    27781 tttagcgcct tgttggcggt gattggtgcc gggattattc gctatgatca ggtcaccgac
    27841 catttctgga ctggcttatt gctggtgcaa ctctccaata tcacttttgc catcggcatg
    27901 gtgggttaca aacgtctgat ggaaactcgc ccgatgccgc agcataacgc ctttgcgtgg
    27961 ttctatcttg gcgcgttcct ggtggcagtg attgcatggt tcttgctggg aaatgcgcag
    28021 aaaatgccgc aaaccacgtt gcaatggggc attctggtgt ttcttggcgt ggtggcttcc
    28081 gggattggct actttatgtg gaactacggc gcgacgcagg tggacgccgg aacgctgggc
    28141 attatgaata atatgcacgt tccggcaggg ctgctggtaa acctggctat ctggcaccaa
    28201 cagccgcact ggccaacgtt tattacaggc gcgctggtga tcctggcctc actgtgggtg
    28261 catcgtaagt gggtcgctcc gcgctcttca caaacggcag atgatcgcag gcgtgattgc
    28321 gcgctgaacg aataaacgct tctgtaatcg gctgacgctg ctcgccatcg cgcacggcgg
    28381 cgtacagtcg gctccacaag ccttcgccca gggttttggt gaccaccaga ccctggcgtt
    28441 caaaactctc caccacccaa tgcggtagtg cggcaatacc catccgtgcg gcaaccatct
    28501 gaatcaacaa taaggtgtta tcgacgcttt tcagtgacgg gctgacgcct gctggctgaa
    28561 gaaaatgcct ccagacatcc agtcggctac gctgcaccgg ataaatcaac agcgtttcgc
    28621 tggcgagatc ttccggtgta atacgcgttt ttgccgccag tggatggtca ggtgctaaca
    28681 ccagacgcac ttcatagtcg aacatcggcg aataatgcag gccactgcgc ggcagaatat
    28741 cggacgtcat caccagatcc agctcgccct gttgcaacgc aggctgcgga tcaaacgtta
    28801 cgcccgattt aaaatccatc tccacctgcg gccagttctt atggaaactt tctaatgcgg
    28861 gcgtcagcca ctgaatacag ctatggcact ctatggcaat gcgcaggcgc gtctgctgcg
    28921 gttcattgca ggcttgcagt gcctggctaa tttgcggcag cacctgattt gccagttgca
    28981 acaggatttc tccctgcggt gtaaagcgaa gcggctggct tttacgcaca aatagccgga
    29041 agccaaggcg ttgttccaga tcgctaaact ggtgagacag ggcggattgc gtttgatgca
    29101 acgtcgccgc agcggctgcg agtgagccgc agttccgcaa cgcttgtagc gttttcaggt
    29161 gttttacttc gatcatgaaa gtccttcact tcggcatgaa taatttgcgc ttgaggaata
    29221 tacagtaacc gccaattatg gatgtgtaaa catctggacg gctaaatact aaaaattcca
    29281 taaggggcat ataatgacaa ttcttaatca caccctcggt ttccctcgcg ttggcctgcg
    29341 tcgcgagctg aaaaaagcgc aagagagtta ttgggcgggg aactccacgc gtgaagaact
    29401 gctggcggta gggcgtgaat tgcgtgctcg tcactgggat caacaaaagc aagcgggtat
    29461 cgacctgctg ccggtgggcg attttgcctg gtacgatcat gtactgacca ccagtctgct
    29521 gctgggtaat gttccgccac gtcatcagaa caaagatggt tcggtagata tcgacaccct
    29581 gttccgtatt ggtcgtggac gtgcaccgac tggcgaacct gcggcggcag cggaaatgac
    29641 caaatggttt aacaccaact atcactacat ggtgccggag ttcgttaaag gccaacagtt
    29701 caaactgacc tggacgcagc tgctggagga agtggacgag gcgctggcgc tgggccacaa
    29761 ggtgaaacct gtgctgctgg ggccgattac ctacctgtgg ctgggtaaag tgaaaggtga
    29821 acagtttgat cgcctgagcc tgctgaacga cattctgccg gtttatcagc aagtgctggc
    29881 agaactggcg aaacgcggca tcgagtgggt acagattgat gaacccgcgt tggtactgga
    29941 actgccgcag gcgtggctgg acgcatacaa acccgcttac gacgcgctcc agggacaggt
    30001 gaaactgctg ctgaccacct attttgaagg cgtaacgcca aacctcgaca cgattactgc
    30061 gctgcctgtt cagggtctgc atgtcgatct cgtacatggt aaagatgacg ttgctgaact
    30121 gcacaagcgt ctgccttctg actggctgct gtctgcgggt cttatcaatg gtcgtaacgt
    30181 ctggcgcgcc gatcttaccg agaaatatgc gcaaattaag gacattgtcg gcaaacgcga
    30241 tttgtgggtg gcatcttcct gctcgttgct gcacagcccc atcgacttga gcgtggaaac
    30301 gcgtcttgat gcagaagtga aaagctggtt tgccttcgcc ctgcaaaaat gtcatgaact
    30361 ggcattgctg cgcgatgcgt tgaacagtgg tgatacggca gctctggcag agtggagcgc
    30421 tccgattcag gcgcgtcgtc actctactcg tgtacataat ccggcagtag aaaagcgtct
    30481 ggcggcgatc accgcccagg acagtcagcg tgcgaatgtc tatgaagtgc gtgctgaagc
    30541 tcagcgtgcg cgttttaaac tgcccgcgtg gccgaccacc acgattggtt ccttcccgca
    30601 aaccacggag attcgtaccc tgcgtctgga ttttaaaaag ggtaatctcg acgccaataa
    30661 ctaccgcacg ggcattgcgg aacatatcaa gcaggccatt gttgagcagg aacgtttggg
    30721 actggatgtg ctggtacatg gcgaggccga gcgtaatgac atggtggaat actttggcga
    30781 gcatctggat ggctttgtct ttacgcaaaa cggttgggta cagagctacg gttcccgctg
    30841 cgtgaagcca ccgattgtta ttggtgacgt tagccgcccg gcaccgatta ccgtggagtg
    30901 ggcaaaatat gcgcaatccc tgactgataa accggtgaaa gggatgttga ccggcccggt
    30961 gactattctc tgctggtcgt tcccgcgtga agatgtcagc cgtgaaacca tcgccaaaca
    31021 aattgcgctg gcgctgcgtg atgaagtcgc ggacctggaa gccgctggaa ttggcatcat
    31081 tcagattgac gaaccggcat tgcgcgaagg tttaccactg cgtcgcagcg actgggatgc
    31141 ctatctccag tggggcgtgg aggctttccg tatcaacgcc gccgtggcga aagatgacac
    31201 acaaatccac actcacatgt gttactgcga gttcaacgac atcatggatt cgattgcggc
    31261 gctggacgca gacgtcatca ccatcgaaac ctcgcgttcc gacatggagt tgctggagtc
    31321 gtttgaagag tttgattatc caaatgaaat cggtcctggc gtctatgaca ttcactcgcc
    31381 aaacgtaccg agcgtggaat ggattgaagc cttgctgaag aaagcggcaa aacgcattcc
    31441 ggcagagcgt ctgtgggtca acccggactg tggcctgaaa acgcgcggct ggccagaaac
    31501 ccgcgcggca ctggcgaaca tggtgcaggc ggcgcagaat ttgcgtcggg ggtaaaatcc
    31561 aaaccgggtg gtaataccac ccggtctttt ctcattacag cgacgtcttc ccagcatact
    31621 gcgtaaacca ttccagatat cagttcatac cctgtttaag aaaatcttct ttattccctt
    31681 ctcatgaaat aaatttcgtc atttccgcca tt
//
LOCUS       DAZFJL010000043        31034 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZFJL010000043 DAZFJL010000000
VERSION     DAZFJL010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.043"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            75..332
                     /gene="bhsA"
                     /locus_tag="ACHJ8C_001940"
                     /gene_synonym="ycfR"
     CDS             75..332
                     /gene="bhsA"
                     /locus_tag="ACHJ8C_001940"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO5551801.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            complement(414..1376)
                     /gene="ldtC"
                     /locus_tag="ACHJ8C_001941"
     CDS             complement(414..1376)
                     /gene="ldtC"
                     /locus_tag="ACHJ8C_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO5551802.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALQAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(1520..4966)
                     /gene="mfd"
                     /locus_tag="ACHJ8C_001942"
     CDS             complement(1520..4966)
                     /gene="mfd"
                     /locus_tag="ACHJ8C_001942"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO5551803.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEEINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAIQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTMRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(5094..6167)
                     /locus_tag="ACHJ8C_001943"
     CDS             complement(5094..6167)
                     /locus_tag="ACHJ8C_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO5551804.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVICKIF
                     NRLALPLFVLFVLMSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTCVKAVPLR
                     RHLLMASLLTVLAVGAWLFNISLLLSLVSIVVIMKLFYQYEQRFGMRSSSLLNVIGSN
                     TIAIYTTHRILVEIFSLSLIPQMNAVRWSPQVELTLLLIYPFVSLLICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPATVSYSR"
     gene            6429..7628
                     /gene="lolC"
                     /locus_tag="ACHJ8C_001944"
     CDS             6429..7628
                     /gene="lolC"
                     /locus_tag="ACHJ8C_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO5551805.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            7621..8322
                     /gene="lolD"
                     /locus_tag="ACHJ8C_001945"
     CDS             7621..8322
                     /gene="lolD"
                     /locus_tag="ACHJ8C_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO5551806.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            8322..9566
                     /gene="lolE"
                     /locus_tag="ACHJ8C_001946"
     CDS             8322..9566
                     /gene="lolE"
                     /locus_tag="ACHJ8C_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO5551807.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWHNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLKLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            9595..10506
                     /gene="nagK"
                     /locus_tag="ACHJ8C_001947"
     CDS             9595..10506
                     /gene="nagK"
                     /locus_tag="ACHJ8C_001947"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO5551808.1"
                     /translation="MYYGVDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADRRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLAERLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            10522..11343
                     /gene="cobB"
                     /locus_tag="ACHJ8C_001948"
     CDS             10522..11343
                     /gene="cobB"
                     /locus_tag="ACHJ8C_001948"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO5551809.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQTFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(11481..12269)
                     /locus_tag="ACHJ8C_001949"
     CDS             complement(11481..12269)
                     /locus_tag="ACHJ8C_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFO5551810.1"
                     /translation="MKKIIILLSLLILLPLTATSNPLIPIMKTLFTDVTGTVPDAEEI
                     AHKAELFRQQTGVAPFIVVLPDINNEASLRQNGKAMLAHAASSMSNVKGSVLLLFTTR
                     EPRLIMITNGQVESSMDDKHLGLLVENHTLAYLHADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQQENDPPGSTTTLGLFAWAVAFIVFAAFFNYTTRLYYALKFAVAMAV
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLWTSKR"
     gene            complement(12266..12727)
                     /locus_tag="ACHJ8C_001950"
     CDS             complement(12266..12727)
                     /locus_tag="ACHJ8C_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFO5551811.1"
                     /translation="MSQDSRVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFIAVGLLISMPSSKKTRRKRKSRP"
     gene            complement(12785..13831)
                     /gene="potD"
                     /locus_tag="ACHJ8C_001951"
     CDS             complement(12785..13831)
                     /gene="potD"
                     /locus_tag="ACHJ8C_001951"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO5551812.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGSAS
                     SIYEEYYQKLKAGR"
     gene            complement(13828..14622)
                     /gene="potC"
                     /locus_tag="ACHJ8C_001952"
     CDS             complement(13828..14622)
                     /gene="potC"
                     /locus_tag="ACHJ8C_001952"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO5551813.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDRTKGNTGDVK"
     gene            complement(14619..15476)
                     /gene="potB"
                     /locus_tag="ACHJ8C_001953"
     CDS             complement(14619..15476)
                     /gene="potB"
                     /locus_tag="ACHJ8C_001953"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO5551814.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            complement(15460..16596)
                     /gene="potA"
                     /locus_tag="ACHJ8C_001954"
     CDS             complement(15460..16596)
                     /gene="potA"
                     /locus_tag="ACHJ8C_001954"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO5551815.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            16846..18072
                     /gene="pepT"
                     /locus_tag="ACHJ8C_001955"
     CDS             16846..18072
                     /gene="pepT"
                     /locus_tag="ACHJ8C_001955"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO5551816.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(18121..19242)
                     /gene="roxA"
                     /locus_tag="ACHJ8C_001956"
                     /gene_synonym="ycfD"
     CDS             complement(18121..19242)
                     /gene="roxA"
                     /locus_tag="ACHJ8C_001956"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO5551817.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(19318..20778)
                     /gene="phoQ"
                     /locus_tag="ACHJ8C_001957"
     CDS             complement(19318..20778)
                     /gene="phoQ"
                     /locus_tag="ACHJ8C_001957"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO5551818.1"
                     /translation="MKKLLHLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(20778..21449)
                     /gene="phoP"
                     /locus_tag="ACHJ8C_001958"
     CDS             complement(20778..21449)
                     /gene="phoP"
                     /locus_tag="ACHJ8C_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO5551819.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(21618..22988)
                     /gene="purB"
                     /locus_tag="ACHJ8C_001959"
     CDS             complement(21618..22988)
                     /gene="purB"
                     /locus_tag="ACHJ8C_001959"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO5551820.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLEELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(22992..23633)
                     /gene="hflD"
                     /locus_tag="ACHJ8C_001960"
     CDS             complement(22992..23633)
                     /gene="hflD"
                     /locus_tag="ACHJ8C_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO5551821.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDGDALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(23669..24775)
                     /gene="mnmA"
                     /locus_tag="ACHJ8C_001961"
                     /gene_synonym="trmU"
     CDS             complement(23669..24775)
                     /gene="mnmA"
                     /locus_tag="ACHJ8C_001961"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO5551822.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKDGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDADRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(24829..25290)
                     /gene="nudJ"
                     /locus_tag="ACHJ8C_001962"
     CDS             complement(24829..25290)
                     /gene="nudJ"
                     /locus_tag="ACHJ8C_001962"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO5551823.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(25300..25953)
                     /gene="rluE"
                     /locus_tag="ACHJ8C_001963"
     CDS             complement(25300..25953)
                     /gene="rluE"
                     /locus_tag="ACHJ8C_001963"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO5551824.1"
                     /translation="MRQFIISENTMQKTSFRNHKVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAEMVEEPEWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGNYSLDNLANGEWRDATD"
     gene            26125..27375
                     /gene="icd"
                     /locus_tag="ACHJ8C_001964"
     CDS             26125..27375
                     /gene="icd"
                     /locus_tag="ACHJ8C_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO5551825.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVINFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWVEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(27459..27584)
                     /locus_tag="ACHJ8C_001965"
     CDS             complement(27459..27584)
                     /locus_tag="ACHJ8C_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5551826.1"
                     /translation="MEIKVQRLSLWMINTVFLLLPINNHQTNTIKLIFEMCSLDF"
     gene            complement(27637..28041)
                     /locus_tag="ACHJ8C_001966"
     CDS             complement(27637..28041)
                     /locus_tag="ACHJ8C_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO5551827.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIRGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(28262..28993)
                     /gene="bluR"
                     /locus_tag="ACHJ8C_001967"
     CDS             complement(28262..28993)
                     /gene="bluR"
                     /locus_tag="ACHJ8C_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO5551828.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQNTQDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGT
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            complement(29198..30409)
                     /gene="bluF"
                     /locus_tag="ACHJ8C_001968"
     CDS             complement(29198..30409)
                     /gene="bluF"
                     /locus_tag="ACHJ8C_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO5551829.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMRSDVTGILLFNG
                     SHFFQLLEGPEEQVQMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSSNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDEEIYTAD
                     LKSKALAFAMAHALKLGDKMISINLLPMTLVNKPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIMKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFSKAKLN
                     GIPSVAWPEKK"
     gene            30723..30959
                     /gene="ycgZ"
                     /locus_tag="ACHJ8C_001969"
     CDS             30723..30959
                     /gene="ycgZ"
                     /locus_tag="ACHJ8C_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO5551830.1"
                     /translation="MHQNSVTSDSAGAITCYFAKANLPTQLETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
BASE COUNT         7731 a         7680 c         7878 g         7745 t
ORIGIN      
        1 atcacatcta tcatttagtt atcgatcgtt aagtaattgc ttgcgacgtc attcatctgc
       61 ataaggccac tattatgaaa aacgtaaaaa ccctcatcgc tgcggcgatt ttaagctcca
      121 tgtcatttgc cagctttgcg gctgtcgaag ttcagtcaac gccagaaggc caacaaaaag
      181 tcggtactat cagtgctaac gcggggacaa atctgggatc gctggaagag cagctggcgc
      241 aaaaagcgga tgagatgggc gcaaaatctt tccgtattac ttctgtaacc ggtccaaata
      301 ccctccatgg aacagcagta atttataaat aagcattaac cctcattaat gcctgctact
      361 gctgattttt tccccgcgac atgccgtgtc gcggggattt ttttatccag gatttacaga
      421 gtttgtgggc tcacttgatg acggcggaca tccaccggca tcccggaacg gacatccatc
      481 acatgttgca tcacttcagc gtcagtttgt gctgcatctt taaatgattg cattgcgcta
      541 ttcagcgtaa ttggcagcag ctgcggatcg tcatcaatct tctctgacag cggctgatgt
      601 acttcaacca gacgcgcacc gtttggttcg gcagagactt ttatcggagt gttgatgata
      661 ttcactttgg tgcctggggt gacctggcta aagagtgttt tgatatcgtc atcccgcaga
      721 cgaatacagc cagaacttac ccgcatgcca atgccgaaat cggcgttcgt accgtggagc
      781 aaataaacgc cgccataggc cgccaggcga atcgcatgat ggcccattgg attatccggt
      841 ccagccggaa ctaccgcagg caactcaatt ccctgtgctt tataacgggc gcggatgttt
      901 gccgttggcg tccaggttgg gtttgcacgt ttgtctgaaa cggtggtcac cattgtcggc
      961 gtcagcgtgt caccacctaa ctgaccaatg ccgatgggat acacggttac cgaattttta
     1021 cccggcgggt agtaatagag acgcagttcc gcaatattga tcacaatgcc ttcgcgcggc
     1081 gcatctggaa gtagggtttg caacgggatc gttaacacgc tgcccgcgcg cggtacgtaa
     1141 ggatcaacgc cggggttagc ctgtaacaga gcgagaaagc cgacgttata ttttttggcg
     1201 atggcttcca gagaaccacc gtcattttcc accacatgaa atttgttttc gccaaccaga
     1261 cgactgcccg ctggcggcag cggccaggtg ttggcttttg cctgtagcgc cagcgccacg
     1321 gcagcggcga acgtaaaaaa cgttagccag cgagaaaaac gcgttttgat catcaccaaa
     1381 aatccataat aaatataagg ttattgtaat aaacagataa gcctgaatta tggatggtga
     1441 cagtgtcgga tagtgcaggg aagtgcaaag aatttgtaaa tgttgcagat gggggcgcag
     1501 aaacgccccc gatttaccat taagcgatcg cgttctcttc cagttcacgc ataaactggc
     1561 gtacccattc gatacgcgtt ttccgctcac tcaaatcctg aataaacttc aggcgcgtcg
     1621 gaccatcgag gcggtaatgc tgcggctgtt tttgcagcaa accaatcaac caggccggat
     1681 taacgtgatt cttctcggca aattcgatca cgccgccttt ctcattacct tccagcttcc
     1741 tgatccccag tttctgcgct tgctggcgca gacgggcaat atccagcagg gtacgcgccg
     1801 gatccggcag caggccgaag cgatcgataa gctcaacttt gatctcttcc agttcgtttt
     1861 ccgttttggc gctggcaata cgtttgtaga acgacagacg cgtgttcacg tcagggatga
     1921 aatcatcggg caatagcgac ggcatccgca gctcgacttc tgtttgctgg ctggtgagat
     1981 cttccagcga cggctcgcgt ccggctttca gcgcatcgac ggcgttttcc agcaactcca
     2041 tatacagcga gaaaccgatg gtttccattg agccgctttg ttcttcgcca agcagttcac
     2101 ctgcgccgcg gatctccaga tcgtgcgttg ccagcgcaaa acctgccccg agatcttcca
     2161 gcgaggcaat cgcttcaaga cgtttttgcg catcggtagt catcgctttt ggatgcggcg
     2221 tcagcagcca tgcatatgcc tggtggtgcg aacgcccgac gcgaccgcgc aactggtgca
     2281 actgtgccag accgaagtga tccgcgcgtt caatgataat ggtgttggcg gtaggaatgt
     2341 cgatcccggt ttcgataatg gtggtacaaa ccagcacgtt gaaacgttga tgatggaaat
     2401 cattcatcac ccgttccagt tcgcgctcac gcatttgccc gtgaccgatg gcaatccgcg
     2461 cttcaggtac cagttctgcc agccgttcgg cggctttctg gatgttttcc acatcattgt
     2521 agagataata aacctgtcct ccgcgcaaaa tttcacgcag gatcgcctcc cggaccacca
     2581 gactgtcata ctcacggaca aaggttttaa ctgccagacg acgggcgggc ggcgtggcga
     2641 taatcgacag gtcacgcatt ccgctcattg ccatattcag cgtacgtggg atcggcgttg
     2701 cagtaagcgt cagaatatcc acgttcgcgc gcatcgcttt aatgcgctct ttatgacgca
     2761 ccccgaagcg gtgttcttca tcgacaatca gcaggcctaa atctttaaac ttgacgtcac
     2821 tttgcagcag tttgtgcgta ccgatcagaa tatcgatttt cccttccgct acttccgcaa
     2881 ggatttgcgt ctgctctttg gcgctgcgga aacgggagat catttcgata cgtaccggcc
     2941 agttggcgaa acggtcgcgg aagttgtcgt aatgctgctg cgcgagaagg gtggttggca
     3001 ccagcaccgc cacctgcttg tggttatcta ctgccaggaa agcggcgcgc atcgccactt
     3061 ctgttttacc aaagccaaca tcgccgcaca ccagacgatc cattgccagc ggctgacaca
     3121 tgtcgctaag caccgcatta atggcctgcg cctgatccgg cgtggtttca aacgggaagc
     3181 tgtcgcagaa caactgatac tgctcgcgat cgtgtttaaa cgcgaagccc tctttggcgg
     3241 cgcgttgcgc gtagatatcc agcaattccg ccgccacatc acgcactttt tccgccgctt
     3301 tctgccgtgc gcgtgaccag gcatcgccac caagtttatg cagtggtgcg ttttcttctg
     3361 cgccacccgc gtaacggcta atcagatgca gtgacgacac cggaacatac agtttggcgt
     3421 cgttggcata ggtgagcatc aaatactcgc cagtaatgcc tccagcttcg agcgtggtca
     3481 ttccggcata acgaccgacg ccgtgctcca gatggaccac cggctgacca atatgcagct
     3541 ccgcaaggtt acggatcagt gtatcggggt tgatggcgcg gcgagaatcc tgacgacgac
     3601 gggcaacgcg ttcaccgagc agatcgcttt cgcaaatcag cgccaggtta cgcatcgtat
     3661 cgacaaaacc atgttcggca gcgccaatca tcagataacg cccacggtcg ctggcttcat
     3721 caagacgcat aatgcgttgc ggagcaattt taattcgcgc gagcagttca cccaacgctt
     3781 cacggcggcc ttcactttct accgagaaca ccaccggacc gtcgaaagac tcgaggaact
     3841 tacgcagcgc atccagcggc gctttttgtt gtgcctgaat ggccaggtct ggcagtttct
     3901 ggaaacctaa attggcattc gctgcttttg tcggtaaatg ttcagttttt agctgcacac
     3961 ggggccagtt tttcagctct gagaagagct catccacccg cagccagagc gattgtggtg
     4021 gcaacagtgg gcgcattggg tcgacgccgc gattctcaaa acgcgccagc gtgtcagcct
     4081 ggaaacgttc ggcactgttt tccagatcgc cagtattcac caacagcgta ttggcaggga
     4141 aataactgaa cagtggcggc agcggttcgc tgaagaatag cggctgccag tactcgatcc
     4201 cggcaggtaa tgtgccttta ctcacttgct gataaatatg ttccgggtcg cgcttcactt
     4261 cgaaggtatc acgccactgg ctgcggaaca gttcaattgc cgctttatcg gtaggaaatt
     4321 cgtgcgcggg aagcagattg atctcttcta cttcctccag cgtgcgctgg ctatcgacgt
     4381 caaacacccg caggctgtcg atttcatcat caaagaaatc aagacgataa ggcagctcac
     4441 tccccatcgg gaagagatcc agcaacgcgc cgcgcgtggc gtattcgccg tgctccatca
     4501 cctggtcaac atggcgataa ccggcgctgt ccagttgggt tcgtaatgca tctcgtgaca
     4561 ggcgctgacc ttttttcatc accagcgcat gaccgtggag aaagctgtgc gggcaaacgc
     4621 gctgcataag cgtattcacc ggaacaatca gtacgccacg ctgcatggtc ggtagctggt
     4681 aaagggtgga aagacgcgag gagataatgt cctgatgagg cgaaaaactg tcgtaaggga
     4741 gggtttccca gtccgccaga ttcatcacca tctgatcggt gaactggctg atttcatcat
     4801 gcaaacgcag agcattttgc atatctggtg caatgagtac caccggaccg gcgtgacgtt
     4861 cggcaatttc cgctaccagc gtcgcgcagg cagcgccggt taactcgccc agcagacgct
     4921 gctcacccgc tttgacgggc agcgtataac gatattgttc aggcataacg gttgtcagat
     4981 tctcgttagg atatgcctca acatatgagg gcatatctct gataagcaat gctgttcatt
     5041 atccgtaatc ggcatggttt agcaaattga atcgccccgg ctggggcgat ggcttagcgg
     5101 gagtaactga cggtagcggg cagagaaggc ggggagaaca acagatcgct gaatgcgcgc
     5161 tgtgaaagtt ttcttaccag caagcccgca acagtacaaa tcaacaaact gacaaagggg
     5221 taaatcagca ggagcgtcag ttcgacttgc ggcgaccagc gtaccgcgtt catttgcgga
     5281 atcaggctta agctgaatat ttctaccaga atgcgatggg tggtatagat agcaatggtg
     5341 ttggagccaa tcacattcag caggctggag gaacgcatac cgaaacgctg ctcgtactga
     5401 taaaacagct tcatgattac cacaatcgat accagcgaca acagcagcga gatattaaac
     5461 aaccaggcac cgaccgccag aacggtcagc aaagaagcca tcagcagatg gcggcgcaac
     5521 ggtaccgctt taacacaggt cattaatgtt gcgccaaacc atgcgccaag gctgtaataa
     5581 ggcaaattgc ggatcacact gttcattccc caccacggtg tgggtacgaa attaacagcc
     5641 acactcatca gtacaaacag aacaaataac ggcagggcca ggcggttaaa aattttacat
     5701 atcacgaaat agacaattaa cgcatacaga taccacaagc tggtgctggc ggtgatcatc
     5761 ccgtgcagga actcaccggt ggaatcggca taagcggcat tggaggcatt gcttaaatcg
     5821 cgctcaggtg ccagccactg gttcagcgca cttaacgcca gccactgcac cacgccccaa
     5881 agcgccagca cccagaagat gttccagatg cgtttatcga gacaatttcc ccacggtacg
     5941 ctgtcgatat agcggcgaat caaatagcca gagataaaga aaaaaaccgg catacgaaag
     6001 ggtgcaaggt aaagattgaa atagatccag catttgctca ggacttctga taacggatgc
     6061 tggaaagtgg tcagatgcgg ataaaaggta atgaccgagt gataaatcac caccagacaa
     6121 atacataacc ctttgatctg gttaatccat agctcttttt gtttcatcag gacaacacgc
     6181 tctttttatt taacatgcgg ttgatcctgc ggggcgagtc gcgtggtgta atgcgtatct
     6241 gtctgtattt ggttattttt cagataaaga tcaggccaca tggccgatga taaaatgagg
     6301 cactttagcc gattgattta cgggggcttt tcagattagc cctgacgatc acttacagtt
     6361 cagacgttta cccatcccgc tttcgcttat atactcgtgt ctttgctaac agcaaccaga
     6421 cggatttcat gtaccaacct gtcgctctat ttattggcct gcgttacatg cgtgggcgtg
     6481 cagcggatcg cttcggtcgt ttcgtctcct ggctttctac catcggcatt accctcgggg
     6541 tgatggcgct ggtcacagta ttgtcagtga tgaacggctt tgagcgcgag ctgcaaaaca
     6601 acatccttgg cctgatgcca caggcaattc tctcttctga gcatggctct cttaacccgc
     6661 agcaactccc ggaaacggca gtcaaactgg acggcgttaa tcgcgtcgca cctattacta
     6721 ccggtgatgt ggtactgcaa agcgcgcgca gcgtggcggt cggggtgatg ctgggtatcg
     6781 acccggcgca aaaagatcca ctaacgccgt atctggtcaa tgtgaaacaa actgacctcg
     6841 agccggggaa atataatgtc atcctcggtg aacagcttgc ctcacagttg ggcgtcaatc
     6901 gcggtgatca aatccgcgtg atggtgccat ctgccagcca gttcacgccg atggggcgta
     6961 ttccaagtca gcgcctgttc aatgtgattg gcactttcgc cgccaacagt gaagtcgatg
     7021 gctatgaaat gctggtgaat attgaggatg cctcgcgtct gatgcgttat ccggcaggca
     7081 atattaccgg ctggcgtttg tggctggatg agccgctgaa agtcgactca ttaagtcagc
     7141 aaaaactgcc tgaaggcagc aaatggcagg actggcgtga ccgtaaaggc gagctgttcc
     7201 aggccgtacg catggaaaaa aatatgatgg gcttgctgct gagcctgatt gtcgccgttg
     7261 cggcatttaa cattattacc tcgctgggac tgatggtgat ggagaagcag ggcgaagtgg
     7321 ccattcttca gactcagggg ctgactccgc gtcaaatcat gatggtcttt atggtgcaag
     7381 gggccagcgc tgggattatc ggtgcgatcc tcggagcggc gcttggcgca ctgcttgcca
     7441 gccagttgaa taatctgatg ccgataatcg gcgtcctgct tgatggcgcg gcgctgccgg
     7501 tcgctatcga gcctttacag gtcattgtta ttgcgctggt ggcgatggct atcgcgctgc
     7561 tgtctacgct ttacccttcc tggcgcgctg ccgccactca acccgctgag gctttacgtt
     7621 atgaataaga tcctgttgca atgcgacaac ctgtgcaaac gctatcagga aggcagtgtg
     7681 caaaccgatg tactgcacaa tgtcagtttc agcgtgggcg aaggtgaaat gatggcgatc
     7741 gtcggcagct ctggttccgg taaaagcacc ttgctgcacc tgttaggcgg gctggatacg
     7801 ccaacctccg gcgatgtgat ctttaacggt caaccgatga gcaaactgtc gtcggcggcc
     7861 aaagcggaac tgcgtaacca gaagctggga tttatttatc agtttcacca cctgttgccg
     7921 gattttaccg ccctggaaaa cgtggctatg ccgctgttga ttggcaagaa aaagcccgct
     7981 gaaatcaaca gccgtgcgct ggagatgtta aaagcggtgg ggctggagca tcgtgcgaat
     8041 caccgcccat ctgaactttc tggcggcgaa cgccagcgtg tggcgattgc ccgtgcgctg
     8101 gtcaataacc cgcggctggt actggcggat gaacctactg gtaacctcga tgcacgtaac
     8161 gccgacagca tcttccagtt gcttggggaa ttgaatcgct tgcagggcac cgccttcctg
     8221 gtggttactc acgacctgca actggcgaaa cgtatgagcc gccaactgga aatgcgtgat
     8281 ggacgtctga cggcggaact gagcctgatg ggggcggagt aatggcgatg cctttatcgt
     8341 tattgattgg cctgcgtttt agccgcggac ggcgacgcgg cggcatggtg tcgttgatct
     8401 ccgtcatttc taccattggc attgccctgg gcgtggcggt attgatcgtc ggcttaagcg
     8461 cgatgaacgg ctttgaacgc gaactgaata accgcattct ggcggtagta ccgcacggtg
     8521 aaattgaggc ggtagatcag ccgtggacca actggcagga agcactggat aacgtgcaga
     8581 aagtgccggg cattgccgcc gctgcgccgt atatcaattt caccgggctg gtggaaagtg
     8641 gcgcgaatct gcgcgcaatc caggtgaagg gcgttaaccc gcaacaggaa cagcgtctga
     8701 gcgcattacc ctcgtttgtt cagggggatg cctggcacaa ttttaaagcg ggcgaacagc
     8761 aaattatcat cggcaaaggc gtggcggatg cgctgaaagt gaagcagggc gattgggtgt
     8821 cgattatgat ccccaactcg aatcccgagc ataaactgat gcagccaaaa cgtgtgcgtt
     8881 tgcacattgc cggtattttg cagttgagtg gtcaactcga tcacagtttt gccatgatcc
     8941 cgctggccga tgctcagcaa tatcttgata tgggttccag cgtgtcaggt attgccctta
     9001 aaatgacgga tgttttcaac gccaataagc tggtacgcga tgcgggtgaa gtgaccaaca
     9061 gctatgttta tattaaaagc tggattggta cttacggcta tatgtatcgc gatatccaga
     9121 tgatccgcgc cattatgtat ctggcgatgg tactggtgat tggcgtggcc tgtttcaaca
     9181 tcgtctccac cttagtgatg gcggtgaaag acaagagtgg cgatatcgca gtattaagaa
     9241 cgctgggggc gaaagatggt ttaattcgcg ccatctttgt ctggtatgga ttgctggcag
     9301 ggctgttcgg cagcctgtgt ggtgtggtta tcggcgtggt ggtttcactt aaacttaccc
     9361 cgattattga gtggattgaa aagctgatcg gtcatcagtt cctctccagc gatatctatt
     9421 ttattgactt cttgccatcg gaattgcact ggctggacgt cttctacgta ctggtcacag
     9481 cattgttgct gagtcttttg gcaagttggt atccggcgcg gcgcgccagt aatattgacc
     9541 ctgcgcgagt ccttagcggc cagtaaaggc agtacattaa aacaaggagc ggcaatgtat
     9601 tacggggttg atattggtgg aacaaaaatt gcgctaggcg tgtttgatag cggtcggcag
     9661 ttgcagtggg aaaagcgggt gccgacaccg cgtgacagct atgacgcatt tttagatgca
     9721 gtgtgcgagc tggtagccga agctgaccgg cgttttggct gtaaaggttc tgtcggcatc
     9781 ggtattccgg gaatgccgga aacagaagat ggtacgctgt atgccgccaa tgtccctgct
     9841 gccagcggta aaccgctgcg tgccgacctg agcgcacgtc ttgatcgcga tgtacgcctt
     9901 gataacgatg ccaactgctt tgccctttct gaagcatggg atgatgaatt tactcaatat
     9961 ccactggtga tggggttgat tctcggcacc ggcgttggcg gcgggctgat tttcaacggt
    10021 aaaccaatta ccggtaaaag ctatattacc ggcgagtttg gccatatgcg tctgccggtt
    10081 gatgcgttaa ccatgatggg gctggatttc ccgttacgcc gctgcggctg tggtcagcat
    10141 ggctgcattg aaaattatct gtctggtcgc ggttttgcgt ggctgtatca acactattat
    10201 catcaaccgt tgcaggctcc tgagatcatt gcgctttatg atcaaggcga tgagcaggca
    10261 agggcgcacg ttgagcgtta tctggattta ttagcggttt gtctgggaaa tatcctgacc
    10321 attgttgacc ctgacctggt cgtcattggt ggtggcttat cgaatttccc ggcaatcaca
    10381 acgcaactgg cggaaaggtt gccacgtcat ctcttacctg tagctcgtgt tccgcgcatt
    10441 gaacgcgcgc gccacggtga tgcgggggga atgcgtggtg cggccttcct acatctaacc
    10501 gattaatcaa cagaggttgc tatgctgtcg cgtcggggtc atcggttaag tcgttttcgt
    10561 aaaaataaac gtcgcctgcg cgagcgtttg cgtcagcgta tttttttcag agataaagtg
    10621 gtgccggaag caatggaaaa accaagagta ctcgtactga caggggcagg gatttctgcg
    10681 gaatcaggta ttcgtacctt tcgcgccgca gatggcctgt gggaagaaca tcgggttgaa
    10741 gatgtggcaa cgccggaagg ttttgatcgc gatcctgaac tggtgcaaac gttttataac
    10801 gcccgtcgtc gacagctgca gcagccagaa attcagccta acgctgcaca tcttgcgctg
    10861 gctaaactgc aagatgctct cggcgatcgc tttttgctgg tgacgcagaa tatcgacaat
    10921 ctgcatgaac gcgcaggtaa taccaatgtc attcatatgc atggggaact gctgaaagta
    10981 cgttgttcgc aaagtggcca ggtcctcgac tggaccggag acgttacgcc agaagataaa
    11041 tgccattgtt gccagttccc ggcacccttg cgcccgcacg tagtgtggtt tggcgaaatg
    11101 ccactcggta tggatgaaat ttatatggcg ttgtcgatgg ccgatatttt cattgccatt
    11161 ggtacttcgg ggcatgttta tccggcggct gggtttgttc acgaagcgaa actgcatggc
    11221 gcgcacaccg tggaactgaa tcttgaaccg agtcaggttg gtaatgaatt tgccgagaaa
    11281 tattacggtc cggcaagcca ggtggtgcca gagtttgttg aaaagttgct gaagggatta
    11341 taaagcggga aaaattgcct gatgcgctac gcttgtcagg cctgtgaagg ggcgcaatca
    11401 attgaatttg cgggttttgt gggccggata aggcgttcgc gccgcatccg gcataataca
    11461 tgcgaacttt actgcctctt ttaccgttta ctcgtccata gtaacgcggc aataaacgta
    11521 cagacaccaa tcagccctgc ccagagtggc gatattctgg taatcgcaaa gctgttatcg
    11581 atatacaaac ataaagcctg ataacccata ttggcaacag ccatcgccac tgcaaatttt
    11641 aacgcataat aaaggcgcgt tgtgtagtta aaaaatgcag caaagacgat gaatgcaact
    11701 gcccaggcaa aaaggcctaa tgtcgtggta ctgccgggtg gatcattctc ctgctgttgt
    11761 ccgggatgcg gataatatgt cagtggcggc gtcggttgtt ttaatatctg tgcttgtaaa
    11821 acagccaatg cattattgat accctgatac cagagatccg catgtaaata agccagcgtg
    11881 tgattttcta ccagaaggcc gagatgttta tcgtccatac tgctttcgac ctggccgttg
    11941 gttatcataa ttaaccgtgg ctcgcgggta gtaaagagta acagaacact tccttttaca
    12001 ttactcattg aagatgccgc atgcgccagc atcgctttgc cattttgcct gagactggct
    12061 tcattattaa tgtcgggtaa aaccacaata aacggtgcaa ctccggtttg ttggcgaaaa
    12121 agctcggctt tatgcgcgat ctcttcagca tctggtacag taccggtaac atctgtaaaa
    12181 agagtcttca ttattggaat caatggatta ctggttgccg ttagaggcag gagtatcagt
    12241 aaactgagca atattatgat ttttttcatg ggcgggactt ccgtttcctg cgtgtctttt
    12301 ttgaggaggg catactgatt aataaaccga cggcgataaa aataacgccc ataatgccga
    12361 taaaccccca tttaaaaaaa ttaacccacg ggttatttat ctcagccttt tcaggatgtc
    12421 ctggagaata ataaacatta actttatcgc cttcgctgtc ttcataaaaa tcgctgccaa
    12481 tactcgaatt gaaaattagc gtgtaatctg gcgtcggacg aaaggcaacg acgggatacc
    12541 acgaaccatt tttacccgtg cgatgagaat gactggagtg ccagacggta tcaacaatga
    12601 cgccttcagt atgaatcgcg tctttgctat aggtgaactg gttataaaag ataacaacag
    12661 aaatgaggat cagtaccaga ccgataccga agaatatacg aaagaaaact ctactgtcct
    12721 gactcattag ccacatcctt gctaaataaa aaacgggcgg taataccacc gcccgcttgc
    12781 tgaattaacg tcctgctttc agcttctgat aatactcttc ataaatgctg ctggcggagc
    12841 ctacgtcatt ctgccattca ccatttttaa tggtttcagc atccgggtag agcgttttat
    12901 cgttcgccac ttccggactt aacagcttac gcgccgcaag gtttggcgtt ggataaccga
    12961 tagtttcagc aacctgtttt gccacatccg ggcgcagcag gaagttgatc aatttcagcg
    13021 cgccttcttt gtttttggca tttgccggga tcgccaggct gtccatccag aaaatgccgc
    13081 cttctttcgg ccacaccaca tcaattggcg tacccgcctg gcgtgcaacg aaagctgaac
    13141 cgttccagat catgccgagg ttaacttcgc cttccatata cgggttcgcc gggttatcgg
    13201 agttaaacgc cgcgacgttt ggcatcagtt ttttcagctc gttgtatgca gcttcaatct
    13261 ctttcggatc ggtggtgtta ccggagtagc ccagcttacg cagtgccatc tggaacactt
    13321 cacgggcatc gtcggtcagc agcaggctgc ctttgtactc aggcttccac agatcggccc
    13381 agctggtgac agatttcgga tccaccgcat caccgttaac gccaatcgct gttgcacccc
    13441 agatatacgg aatggaatag tcgttattcg ggtcaaacgg cttgttgagc atgtctggat
    13501 cgaggttgct gaaattgctt aacttcgact tgtcgatctt ctggatcatc ccttctttac
    13561 gcattttatc gacgtaatag gttgaaggaa ccaccaggtc ataggcaccg tctttgtacg
    13621 ttttcagctt cgcgtacatg gtttcgttcg actcgtaagt cgaatagata accttaatac
    13681 cggtttcttt ggtgaactgt tcaagcagtc ctggcggcac gtactcggtc cagttgtaga
    13741 aatacagcgt gttattgtca tcggcgtgag cggcgctcat gcccagtgcc agagcacccg
    13801 ccgcgagcag gtggcgtgac cattttttca tttaacgtcc cctgtgttac ctttcgttct
    13861 atcacgagca ataagctggc tggcaattac catcaccagc gacagcacca gtaatatggt
    13921 tgccagcgcg ttaacttccg gcgatacgcc gactttgacc atcgaataaa tttttaacgg
    13981 cagaatttca taactcggcc cggtgacaaa cgaagaaacc accacgtcgt ccatcgacag
    14041 ggtaaagctt aacacccagc ccgccgctac cgctggcatt gccagtggca gaatgatttt
    14101 tcgcagaatg gtaaattcgc tggcaccgag atctttcgcc gcttccagca ttcgcacgtc
    14161 aaaacctttc aggcgcgaat acactgtcac caccacaaat ggcaggcaga aggtgatatg
    14221 cgagaacagc agcgaccaga agccaagctg aatacccagc agcataaaca gcaccagcag
    14281 cgaaatcgcc atcacgatat ctggtgacat catcaccaca aacagcattc cgctaacgaa
    14341 cggcttacca cgaaagcgat aacggtacag cgcaactgcc gtcagtgaac cgataagcgt
    14401 agcaaacgtc gccgaaaaca ccgccattgt tagtgaatgc tgcgctgcct gtaacaggct
    14461 gtcgttgttc atcagcaggc tataccattt ggtggtaaaa ccctgccagt tgatgccaaa
    14521 gcgcgagctg ttaaaggagt tcacaatcaa aataatgatt gggatataca ggtacgcgta
    14581 gatagcggtc ataaaaccgc cgcgaagcag tcgaccgatc attcgagttc caccttctta
    14641 ttcagcaaac gagaagcgcg ccagtaaacc agcaacatca ggcccattac gatagtcagc
    14701 gtaatgctgg tggctgcacc aaacggccag tcacgaatat tgaggaactg taccttgatg
    14761 acgttaccga tcagcaggtt tttcgcaccg cccatcaggt cggacacata gaacaggccc
    14821 atcgctggca gcatcaccag cagacatccg gcaataattc ccggcatcgt tagtgggata
    14881 atgatgcgga taaaggtctg caacttgctg gcaccgagat cgcgcgccgc ctcaagcaac
    14941 ggcttatcca gtttttcgat actggagtac agcggcatca ccataaacgg cagcagaatg
    15001 taaaccagac cgataatcac cgcactaggc gtgaacatga tgcgaattgg tgtatcgata
    15061 acgcccagcc agagcaaaaa ctcgttgaga tagcctttgg tgctgaggaa aattttcagc
    15121 ccgtaaatac gaatcaatga gttggtccag aacggaacaa tcagcagaaa cagcagcagc
    15181 ggacgcacct tgtgtggcag cttcgccaga aaccaggcaa aaggatagcc cagcaccagg
    15241 caggcgaggg tggcgatcag cgccatattc agcgagtgca atagcacttc aaaatagagc
    15301 ggatcgagca gacgcgtgta gttatccagc gtaaagacca ttttgacgaa actggcgtcg
    15361 tcgcgggtca aaaagctggt gccaatgatc atcaggttgg gcagaaagac aaacaacaca
    15421 agccaaccga caatagtgac aatcactaca ttctggaact tacttgtgtt cttcatcagc
    15481 cagtacgacc tcccagcttt ctacccaatt aatggccatt ttttggtcga gagagtggtc
    15541 aaagtcagga tcgtcttcat tgaagaattc gctgaccatc accatcttgc cattttccag
    15601 ctcaacaacc gactccagcg tcatgccttt gtagttacgc tcgcgaacgt aaccaatcag
    15661 cccttcggcg tggttgtcgt cgttaatctc ttcaacacgt aagtcttccg ggcgcagcag
    15721 aacatgcagt ttttgccccg gttcaacggc gaagttaacg tagatattac attcgcggcc
    15781 ttcaacgttg gcgcgtacgc gctgctcgtc gagacgttcg atgacagtgg cgttaaacat
    15841 attgatttcg ccaataaacc cggcaacaaa caggtttttc ggctcttcgt agatttcacg
    15901 cggcgtgccg tcttgctcaa tgcgaccatc gcgcatcacc acgatcctgt ccgacatggt
    15961 aagtgcttct tcctggtcgt gagttacgaa cacgaatgta atgccaagct tacgctgtaa
    16021 cgctttcagc tcgttctgca tttgcttgcg cagtttgtaa tccagcgctg agagcgactc
    16081 atccagcaac aacagacgag gcttgttaac caccgcgcga gcaatggcga cgcgttgctg
    16141 ttgaccacca gagagctgat gcggtttgcg ttgagcgaag gtttccagct gcaccatccg
    16201 cagggcttcc atcacgcggg gcgtaatttc agcagcgggg gttttttgca tgcgcaaccc
    16261 aaaggccaca ttttcgaaca cggtcatgtg ggggaaaagt gcgtagcttt ggaaaacagt
    16321 gttcacatag cggttttccg ccggaacgtg ggtgatgtcc tcgttatcca gcatgatgcg
    16381 tccggaatca acagtttcca gacctgcaat caggcgaaga acggttgttt taccgcagcc
    16441 agaagggcca agcagcgtga ggaactcgcc attgttgata gtcagatcca gctggggaat
    16501 gacctcttta ccatcaaagc atttgcgaat tcccgccaat tgcaccagcg gtgaaagcga
    16561 actcggttgt ttattcaatt ttttactctg tcccatgtaa acgcaacgga tggcttaccg
    16621 atgcggggtt tgtggttaac caccttggtg actcttaatg agggcggtaa ttctacggca
    16681 aaccgcttga atcgccaatc tttgttgtga attactggct tagctttata ttcattaagg
    16741 taatgctgat aaatattccc gcttgcaggg ctaaaagtga cctgacgcaa tatttgtctt
    16801 ttcttgcttc ttaataatgt tgtcacaaaa agtgagggtg actacatgga taaactactt
    16861 gagcgatttt tgaactacgt gtctctggat acccaatcaa aggcaggggt gagacaggtt
    16921 cccagcacgg aaggccaatg gaagttattg catctgctga aagagcagct cgaagagatg
    16981 gggcttatca atgtgacctt aagtgagaag ggcactttga tggcgacgtt accggctaac
    17041 gtccctggcg atatcccggc gattggcttt atttctcatg tggatacctc accggattgc
    17101 agcggcaaaa atgtgaatcc gcaaattgtt gaaaactatc gcggtggcga tattgcgctg
    17161 ggtatcggcg atgaagtttt atcaccggtt atgttcccgg tgctgcatca gctactgggt
    17221 cagacgctga ttaccaccga tggtaaaacc ttgttaggtg ccgatgacaa agcaggtatt
    17281 gcagaaatca tgaccgcgct ggcggtatta caacaaaaaa acattccgca tggtgatatt
    17341 cgcgtcgcct ttaccccgga tgaagaagtg ggcaaagggg cgaaacattt tgatgttgat
    17401 gccttcgatg cccgctgggc ttacaccgtt gatggtggtg gcgtaggtga actggagttt
    17461 gaaaacttca acgccgcgtc ggtcaatatc aaaattgtcg gtaacaatgt tcacccgggc
    17521 acggcgaaag gagtgatggt aaatgcgctg tcgctggcgg cacgtattca tgcggaagtt
    17581 ccggcggatg aaagcccgga aatgacagaa ggctatgaag gtttctatca cctggcgagc
    17641 atgaaaggca ccgttgaacg ggccgatatg cactacatca tccgtgattt cgaccgtaaa
    17701 cagtttgaag cgcgtaaacg taaaatgatg gagatcgcca aaaaagtggg caaagggtta
    17761 catcctgatt gctacattga actggtgatt gaagacagtt actacaatat gcgcgagaaa
    17821 gtggttgagc atccgcatat tctcgatatc gcccagcagg cgatgcgtga ctgcgatatt
    17881 gaaccggaac tgaaaccgat ccgcggcggt accgacggcg cgcagttgtc gtttatggga
    17941 ttaccgtgcc cgaacctgtt cactggcggt tacaactatc atggtaagca tgagtttgtg
    18001 actctggaag gtatggaaaa agcggtgcag gtgatcgtcc gtattgccga gttgacggcg
    18061 caacggaagt aagcgaaaag ggatgcggca tgtgatgccg catccggctt aacttcaaac
    18121 ttacccttcg aagaaccaat acccgctatt gactagtgcc gcaagcatcg cgagaaatga
    18181 cggatcttcc agtgcatcac caaaattctc cgcggttagc gcaatgttgc tggcgagcgc
    18241 atccagtgcc ggacggtgtg gggaatcgat cttctcacca ttggcataca cgtcgtcgcc
    18301 aatgcgcaat acgcgcagac cccccaggcg caccagcaca tcaccttgtt tcagcgcatc
    18361 gtagatttca tccggctgat aaggcggctc tggcggcgcg atatccagtt catgacgtga
    18421 ctgggatata aactcgccaa accattgctt aaagtgttcc ggctggttga tcaattcgag
    18481 catcatctca cgcagtttat ccatctcttg cggcagaacg tccgcaggat gagcgcgagg
    18541 tggaacatcc ggatcgctgt agtagttgcc gcccagttca cgttgcagca cataatcggc
    18601 aaatccgcta atcagttccc gcgtatttgg tgcgcgaaaa ccgacggaat agttcatcgc
    18661 attttccagc gcgtagcctt catgcgggaa tcctggcgga atataaagaa tatcgccagg
    18721 ctccagctct tcatcgatga tggcttcgaa cggatcgacc tgtaacagat ccgggtgcgg
    18781 gcagtgctgt ttcatttgta gcttttcgcc cactcgccag cgacgacgac cggtaccctg
    18841 aatgataaac acgtcgtact gatcgagatg cgggccgacg ccgccgcccg gtacagaaaa
    18901 agagatcatc agatcatcaa tacgccagtc cggtagttca cggaacggtc gcattagcgc
    18961 ggcggtcggc tcatgccagt ggttcactgc ttgcactaac aatgaccagt tagtttcacc
    19021 gagatgatcg tagctttcga atggaccgtg gctgacctgc catttgccat cctgatgact
    19081 gaccagtcga ctgtcgactt cgctttccat cgccagaccc gccaactcgt ctggagagat
    19141 tgggtcaata aaattattaa agccgcgttt taacaccacc gggcgtttct gccagtgacg
    19201 ttcaagaaaa tcgggccagt taagagtgag ttggtattcc atgttaagca tccacaggct
    19261 ggtatctgca accgattata acggatgctt aacgtaatgc gtgaagtatg gacatattta
    19321 ttcatctttc ggcgcagaat gctggcgacc aaaaatcact tccatccgcg caccgcccag
    19381 catgctctct ccggcgacga ttttaccctc atattgctcg gtgatttcgc gggctaccgc
    19441 cagccccaca ccttgcccag ggcgtaaagt atcaacccgt tgaccacggt cgaaaatgac
    19501 ctctcgcttg cttaatggaa taccggggcc atcatcctcg accacaatat agagatgctc
    19561 gtcggtttgc cttgcagaaa tttcgacaaa ctcgaggcaa tatttacagg cattatccag
    19621 cacattgccc atcacctcga caaaatcgtt ctgctcacca acaaagctga tctctggcga
    19681 aatatcgaga gagatattga cccctttgcg ttgatacact ttgttcagcg ccgaggtgag
    19741 attgtccaac agtggggcga ccggatgcag ctcgcggcta agcaatgtcc cgccgcgcat
    19801 actggcacga tgcaggtagt agccaatttg ctgtgaaatg cggctgattt gctccagcat
    19861 taccggctca gcatcactga cgctcatctt ctcactacgc agagaacgca gcgtactttg
    19921 cagcaccgcc agtggcgttt tcagactatg ggtcaggtcg gtgagcgtcg tacgatattt
    19981 gtcgtaacgt tcgcgttcac tttttaacag tcggttcagg tttcgtacca gactggtcag
    20041 ttctcgcgtt gtggctggat tgagcaattc acggttatgt tcttccagtt cgcggacttc
    20101 ttttgccagg gcttcgatgg ggcgtaaact ccaccaggcg gcgacccaca gcagcgggat
    20161 cactaacagc agattggctg agagcacata gataaaccag ctccagacca tataggaact
    20221 ttttagctcc accggaatgg tatccaccac cacaatggtt aactttggca tccgcgatgt
    20281 tgccgggtag acgtttaccg ccaccgagtg ggtcatctcc gcgtcgtcat catcttcccg
    20341 cacttcctgc aactgttgct gtatcgaatg atctccactc agcaagaggc tggtatcgtt
    20401 aacatccgct tcaatttcat gaaaaccatt cgatttcagc cagtcaggct ggatcatctt
    20461 catcagccag ggcacgtcac gttgcgccca taaaagctgc ccgttctcat cataaattag
    20521 tgtcatcgtg gggctttgct tgtcgatatt ttcgggtaac tcgacatgca acttattgtt
    20581 ttcccacttc gcaagggtat agaacagatt gctctcgcca cgtaacagcc gaaacgtggt
    20641 tttatcgaaa ctgacgctat aaccgatcag cgcaaccatt ccgtaggcaa gcgaaagcac
    20701 caatactact gctgccgttg ctaacagaaa acgtacccgc agcgagagcg ggaaaaaaag
    20761 atgcagtaat tttttcatca gcgcaattcg aacagatagc cctggccgcg aacggtggta
    20821 atcacttctt gcggatattg tgcctgtatt tttttgcgca gacgtcccat cagtacatct
    20881 atggtatgac tttcccgcag ctccgcatcc gggtagagtt gtagcattaa cgaatctttg
    20941 ctgaccactt tgccattatt gcgtatcaac gtttccataa tggtgtattc gaacgcggtc
    21001 agtttgatca cttcgtcatt aatagataat tcacggcggg agagatcaac ctgaaacggt
    21061 ggtagcgaaa tgacctgtga agccagaccg ctattacgcc gcattaatgc ctgcattcgc
    21121 gccatcacct cttcaatatg aaacggttta gtcacgtaat catcagcacc ggcacttaat
    21181 acttcgactt tgtcctgcca gctctcacgg gcggttaata ccagaatcgg cagtgaaaca
    21241 tcattgctac gccagcggcg aatcagtgac agaccatcct cgtcaggcaa tccgagatcg
    21301 acaatcgcaa tatccggtaa atgttcattg agataataat cggcttcttt ggcatcttct
    21361 gcgtcatcga cctgatgacc agcatcctga atctgaactt taagatggtg acgtaacaac
    21421 gcattgtctt caacaaccag tacgcgcatt tttatttctc cctgtcttaa ttattaaaat
    21481 agtgtaacgc gattatgtgg ttatgggggt aaacattaaa taaaccagcg ggaaggggag
    21541 gtaaagtgaa aaaacaaaaa gtggataatc ttaataagca ggccggacag catcgccatc
    21601 cggcactgat ttgaggctta tttcagctca tcaaccatgg tgatggcgcg accaatatag
    21661 tttgccggcg tcatcgcttt caggcgggct ttctcttctt ctggcaacgc cagaccgtcg
    21721 ataaactgct tcatgccttc ggcgtcaacg cgcttaccgc gagtcagctc tttcagcttc
    21781 tcgtacggtt tttcgatgcc atagcgacgc ataactgtct ggattggctc agccagcact
    21841 tcccagttgt gatccagctc ctccagcaga tggtcacggt tcacttccag tttgcttacg
    21901 cctttcaggg tggattgata cgcaatcagc gcataaccga tacccacgcc gaggttacgc
    21961 agcacggtgg agtcggtcag gtcacgctgc cagcgggaaa ctggcagttt gcttgccagg
    22021 tgctgcaata ccgcgttgga aaggcccagg ttcccttcgg agttttcgaa gtcgatcggg
    22081 ttaactttat gcggcatggt ggaagaacca atctcaccag caatggtttt ctgtttgaag
    22141 tggttaaggg cgatataacc ccagacgtca cggtcaaagt cgatcagaat ggtgttgaag
    22201 cgcgcaacgc aatcaaacag ttcggcaatg tagtcgtgcg gttcgatctg ggtggtgtac
    22261 gggttccact gaatacccag cgaggtgacg aactcttcgc tgaactgatg ccagtcaact
    22321 tccgggtaag cggcgatgtg ggcgttgtag ttaccgacag cgccgttgat tttgccgagg
    22381 atctccacct ggttaagctg gcggtactgg cgctccatac ggtaggcgac gttagccatc
    22441 tctttaccaa tggttgacgg cgtggctggc tgaccgtggg tacgggacag cagcgggata
    22501 tcgcggtact gaacggcgag atctttaata ccatcaatca gttgacgcca gtacggcagg
    22561 atcacttcat cacgcgcggt tttcagcatt aatgcgtgag agaggttatt gatatcttcc
    22621 gaagtacagg caaagtggat gaattcagaa accgcatgca gttccgggat ctccgccact
    22681 ttttctttca ggaaatactc aaccgcttta acgtcgtggt tagtggtacg ctcgatggtt
    22741 ttgatgcgtg ctgcatcttc ttcgctgaaa ctggcgacaa ttgcatcaag gtaaccgatt
    22801 gcgtcggcag caaaagcagg aacttccttg atcgctgcgt gcgcggccag tttttgcagc
    22861 caacgtactt caacttgtac acggaatttc agcaaaccgt attcgctgaa aatcccgcgc
    22921 agcgcgctga ctttatcgcc gtagcgtcca tcgacagggg aaacggcggt cagtgaggat
    22981 aattccatag atcacaactc cggggttaaa tgagcaagaa tttgttttgc ctgagtggtc
    23041 aggcgattac gagaaaacat cagttgcaga cgaccgccgc cgacctggtg ccagagcacg
    23101 gcggcgcgaa tgcctgccag cagggttgcg cgaactttcg cctgcacctg tgggctttgc
    23161 agtacagcag gggaaccagt gacctgaatg cgcgggccaa gcgggctgat aacatcaaca
    23221 tagatggcag ccatcgcgct catcagcgtt tcggactgta aatcgaagtg ttcgagctgg
    23281 cgttgcaggc cgttgatccg gttgcccaga gtgtcgagcg cacctttcgc tgaggagagt
    23341 ttgcgctcaa gcaccatcag actgagtgtg tagcgggtta attcggcgtt taagccctgg
    23401 cgactgctgg cattgagtac gccgagcaag gtttccagcc cgacgcgcag gttggcttcg
    23461 ctaccgccaa aaaccgccag cgttgagctg gggttcatat cgataatact gttgagtgag
    23521 acgtgtagcg catcgccatc acaatgcccc tggtgagcga gttgttgcac caggcgtgcc
    23581 gactgacaaa taccggcaag ggcgagggtg atgtcatagt aattctttgc cacgttcact
    23641 gcttccttgt tttaagtaaa gataataatc agaccggcag cggcagacgc tgctcaataa
    23701 taccgccacc gaggcacact tcaccgttat agaaaacggc agactggccc ggcgtcacgg
    23761 cggcaaccgg ctcatcgaaa atcacttcaa tgcgatcagc gtccagcgcc ttgacggtgc
    23821 acgggatgtc ggtctggcga tagcgggttt ttactgtgca acgcatagta ccggtgaacg
    23881 gctcgcgatc gacccagtgc agctgctggg caatcagacc gacagacatc agacgcgggt
    23941 gttcatggcc ttgcgcaaca atcagaatgt tgttttcgac gtctttatcg accacatacc
    24001 acggttcttc ggtgccgtct ttggtgccac cgatacccag acctttacgc tgaccgaggg
    24061 tgtgatacat cagcccctgg tgctcaccaa tttcatcgcc atcgacggta atgattttgc
    24121 ccggttgcgc cgggagataa cggcccagga actcgcggaa tttacgctcg ccgataaagc
    24181 agataccggt agaatctttt ttcttcgcgg tgaccagacc gagatcttca gcaatcttac
    24241 gcacctgcgg tttttccagt tcgccgaccg ggaacaggct ttgcgcaatt tgctcatggc
    24301 tgagcgtata aaggaagtag ctctggtctt tattgctgtc caggccacgc agcaggcggc
    24361 tcttgccatc gacatctgcc cgacgtacgt aatgaccggt agcgatataa tcggcaccta
    24421 aatcttcggc ggcaaattcg aggaaagctt taaatttgat ctctttgttg cacagaatat
    24481 ccggatttgg cgtgcgaccg gctttatatt cggcaaggaa cagttcgaag acgttgtccc
    24541 agtactcggc agcaaagtta acggtgtgca gttcaatgcc gagcttgtcg cagacagcct
    24601 gggcatcagc cagatccgct gccgctgtgc aatattcctc accgtcgtct tcttcccagt
    24661 tcttcataaa caggccttcg acctgatatc cctgttgttg caacagccag gcagaaacgg
    24721 aggaatcgac accgccggac atgccgacga ttactttttt tgcggtttca gacattggat
    24781 cactcacgac attgaacttc aaggcggcgt attctatcac gcagcctttt agatgacacc
    24841 ctttgtaaaa ggccagttaa aatcgccaat catctccagc ggatagcgtt gcccgctttg
    24901 ataacaacga atactttccg ccaccagcgg cgaacgaaga tttgacgcct gtaaaatttc
    24961 ttcggcgctg acccaacggc agcagtcgat atcgctgtca tgaggctgcg tcgggcatat
    25021 ttgctcaagc tcaatggcaa agaggaaacg caaaaacggc gttttatcag gcgcaatcca
    25081 ctgatgcata cgaataaagt gttgcggctg cgcgctaatg ccggtttctt cccacagctc
    25141 acgggcggca gcttccacta aggtttcatc ggcttccagg tgcccggcag gttggttcca
    25201 taacgcttta ccattaatcg tctcttcaac gactaaaaat ttgccttctg cgtgcaccac
    25261 gcaagcaacg gtaacgtgcg gtttaaacat tttgcatcct taatctgtcg cgtctcgcca
    25321 ttcgccattg gcaagattat ccaaagagta attccccatc gcatagcgaa tcagtcgcag
    25381 cgtggggaaa ccaacatggg cggtcatgcg gcgcacctgg cgattacgtc cttcatataa
    25441 ggtgatcttc agccagctgg tgggaatact tttgcgctca cgaattggcg gattacgggg
    25501 ccataaccat tccggctcct caaccatctc tgcacctgcg ggcagggtag ggccatcatt
    25561 taaggttacg ccattgcgca acgcttcaag ggcgtcttgt gtgggaatac cttctacctg
    25621 tacataatag attttcccgg tacgtttacc cggctgggtt aaacgcgcct gcaaagcgcc
    25681 gttattggtc agcaccagta acccttcgct gtcgcggtcg agacgacctg ccgcatacac
    25741 cccctgaacc gggatgaatt cttttaatgt tttgcgtccg gcttcatcgg tgaactgcgg
    25801 aagaacatcg tagggtttat tgaacaggat cacacgcgtg ggctggtttt caggttttcg
    25861 tctggtagaa cgttgcgagc tgaatcgctt aaccttgtga tttctaaaag aagttttttg
    25921 catggtattt tcagagatta tgaattgccg cattatagcc taataacgcg catctttcat
    25981 gacggcaaac aatagggtag tattgacaag ccaattacaa atcattaaca aaaaattgct
    26041 ctaaagcatc cgtatcgcag gacgcaaacg catatgcaac gtggtggcag acgagcaaac
    26101 cagtagcgct cgaaggagag gtgaatggaa agtaaagtag ttgttccggc acaaggcaag
    26161 aagatcaccc tgcaaaacgg caaactcaac gttcctgaaa atccgattat cccttacatt
    26221 gaaggtgatg gaatcggtgt agatgtaacc ccagccatgc tgaaagtggt cgacgctgca
    26281 gtcgagaaag cctataaagg cgagcgtaaa atctcctgga tggaaattta caccggtgaa
    26341 aaatccacac aggtttatgg tcaggacgtc tggttgcctg ctgaaaccct tgatctgatt
    26401 cgtgaatatc gcgttgccat taaaggcccg ctgaccactc cggttggtgg cggtattcgc
    26461 tctctgaacg ttgccctgcg ccaggaactg gatctctaca tctgcctgcg tccggtacgt
    26521 tactatcagg gcactccaag cccggttaaa caccctgaac tgaccgatat ggttatcttc
    26581 cgtgaaaact cggaagacat ttatgcgggt atcgaatgga aagcagactc tgccgacgct
    26641 gagaaagtga ttaatttcct gcgtgaagag atgggcgtga agaaaattcg cttcccggaa
    26701 cattgcggta tcggtattaa gccgtgttcg gaagaaggca caaaacgtct ggttcgtgca
    26761 gcgatcgaat acgcaattgc taacgatcgt gactctgtga ccctggtgca caaaggcaac
    26821 atcatgaagt tcaccgaagg cgcgtttaaa gactggggct accagctggc gcgtgaagaa
    26881 tttggcggtg aactgatcga cggcggcccg tggctgaaag ttaaaaaccc gaataccggc
    26941 aaagagatcg tcattaaaga cgtgattgct gatgcattcc tgcaacagat cctgctgcgt
    27001 ccggctgaat atgatgttat cgcctgtatg aacctgaacg gtgactacat ttctgacgcc
    27061 ctggcagcgc aggttggtgg tatcggtatc gcccctggcg caaacatcgg tgacgaatgc
    27121 gccctgtttg aagccaccca cggtactgcg ccgaaatatg ccggacagga caaagtaaac
    27181 ccaggctcta ttattctctc cgctgagatg atgctgcgcc acatgggttg ggtagaagca
    27241 gctgacttaa ttgttaaagg tatggaaggc gcgatcaacg ccaagaccgt aacctatgac
    27301 ttcgaacgtc tgatggaagg cgctaaactg ctgaaatgtt cagagtttgg tgacgcgatc
    27361 atcaagaaca tgtaatctct acatgtgtta agtattgaaa cgggcgtata acacgcccgt
    27421 tgtttttata aatatgttaa ccgttataaa ataacgtatc aaaagtcaag tgaacacatt
    27481 tcaaatatca atttgatcgt attggtctgg tgattattta tggggagcaa taaaaagaca
    27541 gtattaatca tccataggga tagtctctgc acttttattt ccattatgct aatgccttac
    27601 tgaattatga agcatttttt aagctcccaa ctttagctag attaatggtt tattattttc
    27661 tacatcttca atatataaaa gcgtattatc aatggcgtag taactgcgtt tgttatgatt
    27721 aacatcagta acccaccgga aaacgccagc gcctgccagt gttgaacagt attcccgaaa
    27781 tgtagatttt ccgcgaatat gaagcaatgc ggcctctttt attttagctg ggttcttggt
    27841 cgtactaact tttaacaggt tcctggttcc tcttaataac aaaaccgtgt catcgtgagt
    27901 aataattctg atgttatccg tagccagata ataaatgtaa tgtgcaatac ggtgatgttt
    27961 taattctgaa taaaaccagg agaagttttg ctcttttctc acttgctcaa acatcttttg
    28021 aaaaacaacg acctgatcca tcaggataat aacctcttgt taggtgtgag actgcgtagt
    28081 gtgcacgatc ggttttacca cttcaatctg gtctgtcctt tggctgtgat atgtacaaag
    28141 tgtgaaaggg ggattacctg aattctcccg gtgagcattt tgcaacggac cagctccgct
    28201 acaaacgctg ttgtgggttc agattataac attctgtcta aggggcggga taaaggtgaa
    28261 tttagggggc atgaaagatg actttataac cttgctcacc ccagtgttgt aaaagttcgt
    28321 tttgccttct cgttggtgcc gtgcctgtcc agacaatcaa tgtttgcgtt gggaacagtt
    28381 ccgggcgcgg cgattcaatg ggatcagcaa gaacagaaat gtgccatcct gacaatgata
    28441 aacgccatgc ttccagccac aatcgtgctc tctcttcaac attccacgcc atcagaatgg
    28501 cctctttacc gggcttacgg cgcatttcga aaagtgaggc cgctgcgtat tcaattaatg
    28561 cgccgtcaaa catactgctc ataatgcggg aggtgttgtg atcaagcacg agacgctggc
    28621 gaacaggaag gtaaacatga ttaatcaatt gatctactgg gtactctcga cctagtgaaa
    28681 taattctcgc gcgtagtttg gcaggattag ccatacgaag aattgacatc atctcttctt
    28741 gcaagcggct ccagtcatct tgcgtattct ggctggtagt ttccagtaat gctttaactt
    28801 tgcctacagg aacgccatta cttatccaac gcttaatctc ttcgatgcgt tgtatgtctt
    28861 cttcatcaaa gagtcggtgt ccgccttcac tgcgctgtgg ttttaacaaa ccgtagcggc
    28921 gttgccaggc ccggagagtg acaggattaa tcccgcaacg ttcagcaaca tcaccaatgc
    28981 tgtaataagc cacaattcct ccttgcgatc ataaatctcc gtcgcctcta cacgacccaa
    29041 taatactttg tacaatatac gctaaaattg tacaaagtat aaataagatt tcagctaaat
    29101 tggatgaaac attattttta atgtggatta aattaaaaca taacgtattc attttcacga
    29161 tgatttccag aaatcatgcg aaataatgtg ctgaaaatta ttttttctcc ggccacgcaa
    29221 ctgaagggat accattcaat ttagctttgg aaaacagatc tccctgaaac atctcaattc
    29281 ctgcagattc aagccacatc cactcttctg gtgttgccac acccatagca gagacttgaa
    29341 tttcaagtga tgtacagcat ttcatgatcg cctgaataat tgcctgccgt ggtccgcttt
    29401 tatgaacatt ggtaatcaat tcctgactga ttttaattct gtcaggctgg aagcgtgaca
    29461 ggagtaacaa accagcaaaa cctgcgccaa aatgatcaat tgctacactg ataccggcag
    29521 cctttagcga tttaaccgct tcggcaaact catcaaaccg agatatgact tcactttcag
    29581 taaattcaac gatgatttgt tcaggcacca gagcattggc ctttatttca tcaagtaaaa
    29641 aagagactgc gtcaggtttg ttaaccaggg tcataggtaa tagattgatt gaaatcattt
    29701 tatcaccgag cttaagtgcg tgtgccatcg caaatgcaag cgccttactt ttgagatccg
    29761 ctgtgtagat ttcctcgtct ttacgctgcc caaccgctat agctgacggg ctgtcttcat
    29821 ttttttgcac aatggcttca aaagcgatta tccgccgcga taaagggtcg acaataggat
    29881 gaaaggcaaa gtggtcgttt atatttgatg aaagggtaca agaatcaagt tctttatcag
    29941 atccgtcagc gataaaaagc caggagtctt cagcaggaat ctcgaaatag gttgattgtt
    30001 cagttgcaag gacaaaagta cgaaaaaatt gtagcgctct gtcatcataa gttagctgaa
    30061 attttgatgt gcccttgtcg aatacggcct gtaaaacgtc atctcgctcg tgcaggcgta
    30121 aatcaaataa ttccattcct gctttgccaa aacggcgagc aggcgcgtaa tcgcacaaca
    30181 gttcaacaat attatagtgc cgtggatcct ggcatatggc ccgatatatc atttgaacct
    30241 gttcttccgg accttccaga agctggaaaa aatgagaacc attaaacagt aagatccctg
    30301 ttacgtcaga ccgcatgttc ctgcgatttg ctatcgaaac catctcttcg atttttttga
    30361 caggttcgtc gtcacgtata tggctacgat aaataagggt ggtaagcatt aacaatccag
    30421 ggtaatgggc gaggagagag taagacggta acagacatat cttcttgtgt ctttctttta
    30481 ataccaaaac ataaccgttt ctttacattg ataaaaagtg gagaaggttg aagacggttt
    30541 ggcgaaaaaa catgtatagg ttgtcattta aattatgcaa tatgttttat cgtaatgcat
    30601 tgttttatat gaattagcac taattgcgaa aaattaattt atcattctgt acacatattt
    30661 cgtacaagtt tgctattgtt acctcactta acattgatta acatttttaa cagaggcgta
    30721 gcatgcatca aaattcagtg acttcagatt ctgcaggagc aatcacttgt tactttgcaa
    30781 aagctaactt gcctactcag ctggaaactc tcggggaaat tgtgactgag attttgaaag
    30841 atggacgtaa tctgagtcga aagtcgcttt gtgccaaact tctttgccga ctggaacaag
    30901 cgacagggga agaggaacag aaacattata atgcactaat tgggttgctt tttgaataat
    30961 gcaacagcct gattatttaa caggttagct atgaagtcgt tatgaagaca tctgataatg
    31021 aaagtataaa atat
//
LOCUS       DAZFJL010000044        31016 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFJL010000044 DAZFJL010000000
VERSION     DAZFJL010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.044"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            333..899
                     /gene="mepS"
                     /locus_tag="ACHJ8C_001970"
     CDS             333..899
                     /gene="mepS"
                     /locus_tag="ACHJ8C_001970"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO5551831.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            1080..2636
                     /gene="pdeN"
                     /locus_tag="ACHJ8C_001971"
     CDS             1080..2636
                     /gene="pdeN"
                     /locus_tag="ACHJ8C_001971"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO5551832.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDN
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGLALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTFVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVVYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAEAQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPAHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEREATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            2718..4532
                     /gene="yejA"
                     /locus_tag="ACHJ8C_001972"
     CDS             2718..4532
                     /gene="yejA"
                     /locus_tag="ACHJ8C_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO5551833.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNVTTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSHPAVRPVYSLGIDTWWYDVNKATKLPSARQQGE"
     gene            4533..5627
                     /gene="yejB"
                     /locus_tag="ACHJ8C_001973"
     CDS             4533..5627
                     /gene="yejB"
                     /locus_tag="ACHJ8C_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO5551834.1"
                     /translation="MGAYLIRRLLLVVPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFFDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            5627..6652
                     /gene="yejE"
                     /locus_tag="ACHJ8C_001974"
     CDS             5627..6652
                     /gene="yejE"
                     /locus_tag="ACHJ8C_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5551835.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            6654..8243
                     /gene="yejF"
                     /locus_tag="ACHJ8C_001975"
     CDS             6654..8243
                     /gene="yejF"
                     /locus_tag="ACHJ8C_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO5551836.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIAAGETLALVGES
                     GSGKSVTALSILRLLPCPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEKNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSHGSIVFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(8247..8591)
                     /gene="yejG"
                     /locus_tag="ACHJ8C_001976"
     CDS             complement(8247..8591)
                     /gene="yejG"
                     /locus_tag="ACHJ8C_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO5551837.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(8925..10115)
                     /gene="bcr"
                     /locus_tag="ACHJ8C_001977"
     CDS             complement(8925..10115)
                     /gene="bcr"
                     /locus_tag="ACHJ8C_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO5551838.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPQRRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNILFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(10143..10838)
                     /gene="rsuA"
                     /locus_tag="ACHJ8C_001978"
     CDS             complement(10143..10838)
                     /gene="rsuA"
                     /locus_tag="ACHJ8C_001978"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO5551839.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            10988..12748
                     /gene="radD"
                     /locus_tag="ACHJ8C_001979"
     CDS             10988..12748
                     /gene="radD"
                     /locus_tag="ACHJ8C_001979"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO5551840.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIDDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKIDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIATRRCRE
                     CDTILVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTTFEQLFIRPHTRTPGIPLRWITAADVLAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            12873..13157
                     /gene="rplY"
                     /locus_tag="ACHJ8C_001980"
     CDS             12873..13157
                     /gene="rplY"
                     /locus_tag="ACHJ8C_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO5551841.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(13296..14303)
                     /gene="yejK"
                     /locus_tag="ACHJ8C_001981"
     CDS             complement(13296..14303)
                     /gene="yejK"
                     /locus_tag="ACHJ8C_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO5551842.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            14485..14712
                     /gene="yejL"
                     /locus_tag="ACHJ8C_001982"
     CDS             14485..14712
                     /gene="yejL"
                     /locus_tag="ACHJ8C_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO5551843.1"
                     /translation="MPQISRYSDEQVEQLLAELLNILEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            14732..16492
                     /gene="yejM"
                     /locus_tag="ACHJ8C_001983"
     CDS             14732..16492
                     /gene="yejM"
                     /locus_tag="ACHJ8C_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO5551844.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            16567..16643
                     /locus_tag="ACHJ8C_001984"
     tRNA            16567..16643
                     /locus_tag="ACHJ8C_001984"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16601..16603,aa:Pro,seq:ggg)
     gene            complement(16770..17698)
                     /locus_tag="ACHJ8C_001985"
                     /pseudo
     CDS             complement(16770..17698)
                     /locus_tag="ACHJ8C_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            18018..18665
                     /gene="narP"
                     /locus_tag="ACHJ8C_001986"
     CDS             18018..18665
                     /gene="narP"
                     /locus_tag="ACHJ8C_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO5551845.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDSGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(18700..19752)
                     /gene="ccmH"
                     /locus_tag="ACHJ8C_001987"
     CDS             complement(18700..19752)
                     /gene="ccmH"
                     /locus_tag="ACHJ8C_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO5551846.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYVRSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(19749..20306)
                     /gene="dsbE"
                     /locus_tag="ACHJ8C_001988"
     CDS             complement(19749..20306)
                     /gene="dsbE"
                     /locus_tag="ACHJ8C_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO5551847.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(20303..22246)
                     /gene="ccmF"
                     /locus_tag="ACHJ8C_001989"
     CDS             complement(20303..22246)
                     /gene="ccmF"
                     /locus_tag="ACHJ8C_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO5551848.1"
                     /translation="MMPEIGNGLLCLALGIALLLSGYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVNPQKTAPEAA"
     gene            complement(22243..22722)
                     /gene="ccmE"
                     /locus_tag="ACHJ8C_001990"
     CDS             complement(22243..22722)
                     /gene="ccmE"
                     /locus_tag="ACHJ8C_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO5551849.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(22719..22928)
                     /gene="ccmD"
                     /locus_tag="ACHJ8C_001991"
     CDS             complement(22719..22928)
                     /gene="ccmD"
                     /locus_tag="ACHJ8C_001991"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO5551850.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(22925..23662)
                     /gene="ccmC"
                     /locus_tag="ACHJ8C_001992"
     CDS             complement(22925..23662)
                     /gene="ccmC"
                     /locus_tag="ACHJ8C_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO5551851.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(23704..24366)
                     /gene="ccmB"
                     /locus_tag="ACHJ8C_001993"
     CDS             complement(23704..24366)
                     /gene="ccmB"
                     /locus_tag="ACHJ8C_001993"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO5551852.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAAVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(24363..24986)
                     /gene="ccmA"
                     /locus_tag="ACHJ8C_001994"
     CDS             complement(24363..24986)
                     /gene="ccmA"
                     /locus_tag="ACHJ8C_001994"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO5551853.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTGAA"
     gene            complement(24999..25601)
                     /gene="napC"
                     /locus_tag="ACHJ8C_001995"
     CDS             complement(24999..25601)
                     /gene="napC"
                     /locus_tag="ACHJ8C_001995"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO5551854.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(25611..26060)
                     /gene="napB"
                     /locus_tag="ACHJ8C_001996"
     CDS             complement(25611..26060)
                     /gene="napB"
                     /locus_tag="ACHJ8C_001996"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO5551855.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(26057..26920)
                     /gene="napH"
                     /locus_tag="ACHJ8C_001997"
     CDS             complement(26057..26920)
                     /gene="napH"
                     /locus_tag="ACHJ8C_001997"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO5551856.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(26907..27602)
                     /gene="napG"
                     /locus_tag="ACHJ8C_001998"
     CDS             complement(26907..27602)
                     /gene="napG"
                     /locus_tag="ACHJ8C_001998"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO5551857.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTAKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(27609..30095)
                     /gene="napA"
                     /locus_tag="ACHJ8C_001999"
     CDS             complement(27609..30095)
                     /gene="napA"
                     /locus_tag="ACHJ8C_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO5551858.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEDKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGSNMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYDVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(30092..30355)
                     /gene="napD"
                     /locus_tag="ACHJ8C_002000"
     CDS             complement(30092..30355)
                     /gene="napD"
                     /locus_tag="ACHJ8C_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO5551859.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFTGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(30345..30839)
                     /gene="napF"
                     /locus_tag="ACHJ8C_002001"
     CDS             complement(30345..30839)
                     /gene="napF"
                     /locus_tag="ACHJ8C_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO5551860.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            30948..>31016
                     /gene="yojO"
                     /locus_tag="ACHJ8C_002002"
     CDS             30948..>31016
                     /gene="yojO"
                     /locus_tag="ACHJ8C_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFO5551861.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKR"
BASE COUNT         7538 a         8502 c         7740 g         7236 t
ORIGIN      
        1 agcgcaaaaa tcattctcta agtaaatgaa tggattgcat gcgtttcact caatcgtact
       61 ttaattgacc aatcccgctt attaactttc tgtatcactt tttcttataa aaaatcatgt
      121 aaaaccgctc gccaagaccg caccaatcgg gtaatctcga actcgttttg cctcggcggt
      181 agattatcct cacagcatat aattttgtgc gttagtccac agatttggcc ttaaggaatt
      241 gtttcaacat gcccaggtaa ttagtctcgt gtcgcttggc atttttttat aacgatattt
      301 gtcgttaagg acttcaaggg aaaacaaaca acatggtcaa atctcaaccg attttgagat
      361 atatcttgcg cgggatcccc gcgattgcag tagcggttct gctttctgca tgtagtgcaa
      421 ataacaccgc aaagaatatg catcctgaga cacgtgcagt gggtagtgaa acatcatcac
      481 tgcaagcttc tcaggatgaa tttgaaaacc ttgttcgtaa tgtcgacgta aaatcgcgaa
      541 ttatggatca gtatgctgac tggaaaggcg tacgctatcg tctgggcggc agcactaaaa
      601 aaggtatcga ttgttctggt ttcgtacagc gtacattccg tgagcaattt ggcttagaac
      661 ttccgcgttc gacttatgaa caacaggaaa tgggtaaatc tgtttcccgc agtaatttgc
      721 gcacgggtga tttagttctg ttccgtgccg gttcaacggg acgccatgtc ggtatttata
      781 tcggcaacaa ccagtttgtc catgcttcca ccagcagtgg tgttattatt tccagcatga
      841 atgaaccata ctggaagaag cgttacaacg aagcacgccg ggttctcagc cgcagctaat
      901 aaaccgtttg gatgcaatcc cttggctatc ctgacgagtt aactgaaagc actgcttagg
      961 cagtgctttt ttgttttcat tcatcagaga aaatgatgtt tccgcgtctt gatccaggct
     1021 atagtccggt cattgttatc ttttaaatgt tgtcgtaatt tcaggaaatt aacggaatca
     1081 tgtttatacg cgctcccaat tctggacgta agctcctgct tacctgcatt gttgcaggcg
     1141 tgatgattgc gatactggtg agctgccttc agtttttagt ggcctggcat aagcacgaag
     1201 tcaaatacga cacactgatt accgacgtac aaaagtatct cgatacctat tttgccgacc
     1261 ttaaatccac tactgaccgg ctccagccgc tgaccttaga tacctgccag caggctaacc
     1321 ccgaactgac cgcccgcgcg gcgtttagca tgaatgttcg tacgtttgtg ctggtaaaag
     1381 ataataaaac attctgttca tctgcaactg gtgagatgga cattccactc aatgaattga
     1441 ttccggcgct cgacattaat aaaaacgtcg atatggcgat cttacccggc acgccgatgg
     1501 tgccgaacaa acccgcaatc gtcatctggt atcgcaaccc tttgctgaaa aatagcggcg
     1561 tgtttgccgc tctgaatctc aacctgacgc cttcactctt ttatagttca cggcaggaag
     1621 attacgatgg cctcgccctc attattggta atactgcgct atctaccttt tcttcacgtt
     1681 tgatgaacgt taatgaatta accgacatgc cggtccgtga aactaaaatt gcgggcatac
     1741 ctctgactgt tcggctttat gcagatgact ggacatggaa cgatgtgtgg tacgcatttt
     1801 tactgggtgg catgagtgga actttcgttg gacttctctg ctattacctg atgagtgtgc
     1861 gtatgcgccc aggcagagaa atcatgaccg ccatcaagcg cgaacaattt tacgtggtgt
     1921 atcaaccggt ggttgataca caagctttgc gggtaacggg cctggaagta ctgctacgct
     1981 ggcggcatcc agtagcagga gaaatacccc cggatgcctt cattaacttt gccgaagcgc
     2041 aaaagatgat tgtgccactg actcagcacc tgtttgagtt gattgcccgc gatgccgcag
     2101 aattagaaaa agtactgccg gtaggcgtca aatttggcat taacattgcg ccggcccact
     2161 tgcacagcga aagctttaaa gcggatatcc agaaactgct cacttccctg cccgcacacc
     2221 atttccagat tgtgctggaa attaccgagc gcgatatgct gaaagagcga gaagccacac
     2281 aactcttcgc ctggctgcat tcggtcggcg tagaaattgc tattgatgac ttcggcaccg
     2341 ggcacagcgc gcttatctat cttgagcgtt ttacgctcga ttatctgaaa attgatcgtg
     2401 gatttatcaa cgccatcggt acggaaacga tcacttcacc cgtacttgac gcggtgctga
     2461 cgctggcgaa acgtctcaat atgctgacag ttgctgaagg ggtcgaaacg ccagaacagg
     2521 cacgatggct aagcgaacgc ggcgttaatt tcatgcaagg ctactggatt agtcgcccgt
     2581 taccgctgga cgattttgtt cgctggctga agaaaccgta tacgccgcag tggtaaggtg
     2641 tgcttacgtc ccttattatt catagtgaaa gcatgccgga ttgcggctaa tgatgagtaa
     2701 aaggaaatcc gttgcagatg attgtgcgca tactgctgct gtttatcgct ctgttcacct
     2761 ttggtgcgca ggcgcaggct atcaaggaaa gctatgcctt tgccgtactg ggcgaacccc
     2821 ggtacgcatt taatttcaac cattttgatt atgtgaaccc cgccgcgcca aaaggtgggc
     2881 aaataacgtt gtctgccctc ggcaccttcg ataatttcaa ccgctatgca ctgcgcggca
     2941 atccgggcgc acgcaccgag cagctgtacg acacgctatt tacgacttcc gatgacgaac
     3001 caggcagtta ttacccgctg attgctgaaa gcgcacgcta tgctgacgat tattcctggg
     3061 tggaggtcgc tattaatccg cgcgcccgtt ttcatgatgg ttcgcccatt actgcccgcg
     3121 atgtagagtt tacttttcaa aaatttatga ccgaaggcgt gccgcaattt cgtctggtct
     3181 acaaaggcac caccgtcaaa gccattgcgc cgttaaccgt gcgcattgag ttagctaaac
     3241 ccggcaaaga agatatgctg agtctgtttt cgctgccggt atttccagaa aagtactgga
     3301 aagatcacaa acttagcgac ccgctcgcca cgcctccgct tgccagtggt ccgtaccgca
     3361 tcacgtcctg gaaaatgggg caaaatattg tctattcccg cgtgaaagat tactgggcag
     3421 caaacttacc ggtaaaccgt ggacgctgga atttcgacac cattcgctac gattattacc
     3481 tcgatgataa tgtcgccttt gaagcgttta aagcaggtgc ctttgatttg cgtatggaaa
     3541 acgacgccaa aaactgggcc acgcgctata ccggtaaaaa tttcgataaa aaatacatca
     3601 tcaaagatga gcaaaagaac gaatcagccc aggatacgcg ctggctggcg tttaatatcc
     3661 aacgtccggt attcagcgat cgccgggtgc gggaagcgat cactctcgcc tttgactttg
     3721 aatggatgaa caaagcgttg ttttacaatg cctggagtcg cacgaacagt tattttcaga
     3781 ataccgaata tgcggccaga aattaccccg acgccgcgga actggtgctt ctggcaccaa
     3841 tgaaaaaaga tctaccgcca gaagtcttca cgcaaatcta ccagccgccg gtatccaaag
     3901 gcgatggcta cgatcgtgac aacctgttaa aagccgacaa acttctcaac gaagcaggct
     3961 gggtgctgaa gggtcagcaa cgcgttaatg tcacaacggg tcagccactc agctttgaat
     4021 tattgcttcc cgcaagcagc aatagtcagt gggtattgcc gttccagcac agcctgcaac
     4081 ggctgggtat caacatggac attcgcaagg tggataactc gcaaatcacc aaccgcatgc
     4141 gcagtcgcga ctatgacatg atgccgcgcg tatggcgggc gatgccgtgg cccagttccg
     4201 atttacagat ttcctggtca tcggaatata tcaattccac ttataatgcc cccggcgtgc
     4261 aaagcccggt tatcgactcg ctgatcaacc aaattattgc cgcgcaggga aataaagaaa
     4321 aattactgcc gttggggcga gcactggatc gcgtattaac gtggaattat tacatgctgc
     4381 caatgtggta catggcggaa gaccgtctcg cctggtggga taaattctcc caccccgctg
     4441 tacgccctgt ttacagcctg ggtattgata cctggtggta tgacgttaat aaagcgacga
     4501 aactgccgtc agccagacaa cagggagagt agatgggcgc ttatctgatt cgccgtctgt
     4561 tgctggtggt cccaacgcta tgggcgatta tcactatcaa ctttttcatc gtgcaaattg
     4621 cgcctggcgg tccggtcgac caggccatcg ccgccattga gtttggcaat gccggagtat
     4681 tacccggcgc aggcggtgaa ggtgttcgcg ccagccatgc gcaaacgggc gtcggcaata
     4741 tcagcgacag taattaccgt ggcggacgcg gattagatcc agaagtgatc gccgagatca
     4801 ctcatcgcta cggtttcgat aagccgatcc acgaacgtta cttcaaaatg ctctgggact
     4861 acatccgttt tgattttggc gacagccttt ttcgcagcgc ctcggtgctg acgctgatta
     4921 aagacagtct gccggtctcc atcaccctcg gattgtggag cacgctgatt atctatctgg
     4981 tgtcgattcc gttaggcatt cgcaaagctg tttataatgg gagccgtttt gacgtctgga
     5041 gtagtgcatt tatcatcatc ggctacgcca ttccggcctt tttgtttgcc atcctgctga
     5101 ttgtcttctt cgcaggcggc agctatttcg acctgttccc tctgcgcggc ctggtttccg
     5161 ctaactttga ttcgctgccg tggtatcaga aaatcaccga ttatctgtgg catatcacgc
     5221 tgccggtgct ggcgacagtg attggcggct ttgcggctct gaccatgctg acaaaaaact
     5281 cattctttga tgaagtgcgt aagcaatacg tggtgacggc acgtgcgaaa ggggtaagtg
     5341 aaaaaaatat tctctggaaa catgtgttcc gcaacgccat gctgctggtg attgccggtt
     5401 ttccggcgac gtttatcagc atgtttttta ccggctcgct gctgattgag gtgatgtttt
     5461 cactcaatgg tcttggctta ctgggctacg aagcgaccgt ctcgcgcgat tatcctgtaa
     5521 tgtttggtac cttgtatatt ttcaccctga ttggcctgct gctgaatatt gtcagtgata
     5581 tcagctatac gctggtcgat ccgcgtatag actttgaggg acgttaatgt cgcgactcag
     5641 ccccgtcaat caggcccgtt gggcgcgttt tcgccataac cgtcgcggct actggtcgtt
     5701 atggattttc ctcgtcttgt ttggtttgag tttgtgttct gaacttatcg ccaacgataa
     5761 accgttgctg gtgcgttatg acggcagttg gtatttcccg ttgttgaaaa actacagcga
     5821 aagcgatttt ggaggcccgc tggcaagtca ggctgattat caggacccgt ggctgaaaca
     5881 acggctggaa aataacggct gggtactgtg ggcaccgatt cgctttggtg ctaccagtat
     5941 caactttgct accgataagc ccttcccttc tccaccctcc cggcaaaact ggctgggaac
     6001 ggatgccaat ggcggcgatg tgctggcgcg cattctctat ggcacgcgga tctcagttct
     6061 gtttggcctg atgctgaccc tctgctccag cgtgatgggc gtgctggcgg gggcgctaca
     6121 aggctattac ggtggtaaag tcgatctctg ggggcaacgt tttattgaag tatggtcggg
     6181 gatgccgacg ctgtttttga ttattttact ttccagcgtc gtacagccta acttctggtg
     6241 gctgttggca attaccgtct tgtttggctg gatgagtctg gtcggtgtgg tgcgggcgga
     6301 gtttttacgt acccgtaatt tcgactacat ccgcgcggcg caggcgcttg gcgtcagcga
     6361 tcgcagtatc atcctgcgtc atatgttgcc aaatgccatg gtcgctaccc tcactttttt
     6421 accgtttatt ttatgtagtt cgataaccac cctgacctca ctcgatttcc tcggcttcgg
     6481 tctgccgctc ggttcaccgt cactcggtga actgctgtta caagggaaaa ataaccttca
     6541 ggccccgtgg cttgggatca ccgccttctt atcggtggcg atattgttgt ctttgctgat
     6601 ctttattggt gaagccgtcc gcgacgcatt tgatcctaat aaggcggtgt agcatgacgc
     6661 aaactctgtt agcgattgaa aatttgtcgg tgggttttcg ccatcagcaa accgtacgta
     6721 cagtagtcaa tgatgtttca ctacagattg cggctggcga aacgctggcg ctggtaggtg
     6781 agtctggttc aggtaagagc gttaccgcgc tgtcaatttt acgcctgctc ccttgcccgc
     6841 cggttgaata tctctccggc gatattcgtt ttcatggcga atcgctgctt catgccagcg
     6901 atcaaacgtt gcgcggtgta cgcggtaata agatcgccat gatttttcag gagccgatgg
     6961 tgtcgttaaa tccattacat accctggaaa aacagcttta tgaagtactt tcactccacc
     7021 gcgggatgcg tcgggaagcg gctcgtggcg aaattcttaa ctgccttgat cgcgtcggta
     7081 tccgccaggc ggcaaaacgg ctgacagatt atccgcatca gctctccggc ggcgaacgcc
     7141 agcgggtgat gattgcgatg gcgctgttaa cgcgaccgga attattaatt gccgatgaac
     7201 caaccaccgc gctggacgtc tctgtccagg cgcagatttt acagctgttg cgcgaactgc
     7261 aaggcgagct gaatatgggt atgctgttta ttactcataa cctcagcatt gttagaaaac
     7321 tggcccaccg cgtggcggta atgcaaaacg gtcgctgtgt cgagaaaaat aacgccgcta
     7381 cgctgtttgc ctcacccact catccttaca cacaaaagct actcaacagt gaaccatctg
     7441 gcgacccggt gccattgcca gaacctgcct ctacgttgct agatgttgaa caacttcaag
     7501 ttgccttccc cattcgtaaa gggatcttga agcgcattgt ggatcataat gtggtggtga
     7561 aaaacatcag ttttacgcta cgggcgggtg aaacactggg tttagtgggc gagtccggtt
     7621 ccgggaaaag cacgacggga ctggcgctgc tgcgactgat taattctcat ggcagcatcg
     7681 tctttgacgg tcagccactg caaaatttaa atcgccgcca gctgttacct attcgtcatc
     7741 gcattcaggt ggtatttcag gatccaaact cctcactcaa cccacgactc aacgttttgc
     7801 aaattattga ggaaggctta cgggttcacc agccgacgct ttctgccgca caacgcgaac
     7861 aacaagtgat agccgtgatg catgaagtgg gattagatcc tgaaacacgc caccgttatc
     7921 cggcggagtt ctctggtggt cagcggcaac gtattgcgat tgccagggcg ttaattctta
     7981 agccctcgct gatcattctt gatgaaccaa catcatcact cgacaaaacg gtgcaggcgc
     8041 aaatattgac gctattgaaa tcattgcaac aaaagcatca actggcctat ttgtttatca
     8101 gccacgattt gcacgttgtc cgcgcgttat gtcatcaggt tatcgtactg cgacaagggg
     8161 aagtagtgga acaaggaccg tgcgcgcgcg tgtttgccgc accgcagcag gagtatacgc
     8221 gtcagctact ggcgttgagc tgacgcttaa aaaggattgt agtttgaaaa gggttcggca
     8281 attgccacac caaaattttt caatcggcat gttgcagcaa attcgtcctg gcgatttaca
     8341 aacaggcacg gctcaccttc acactccagc accgaacatg gaacttcgat gtcgcttaac
     8401 gcctggctta gtttgtacat caccgaccat gcattatcac catccgggct aagcaattta
     8461 agcgccacca ggctgttgtc accgcacggt ccattttgcg gaatcggttc aaccttcgaa
     8521 cctgcgaaac cagcagacca gcgatagttc tgcggcagtc gatggacgat tgagagttgt
     8581 aatgaagtca cttattttcc ccggaagtac atttcacttc acaatttgtt tacatcaatt
     8641 ttaacacatc atcaacaatc cgtctttata cagatcgtaa agattgatgc ctcgcctgcg
     8701 tggcctctat ggctctgatt taaataatat ctgcgggcgt ggtcacgttt gcggggctat
     8761 atgtacccgt ttctgcgatc taactcaacc tttttaacta caatgatgtg actttttaca
     8821 taaattgatt ttacataaaa taaacatata tcgggggaat gatagatttg tggttgacat
     8881 gcatcaacaa aggaagcctt ttagcttcct cgttgtgcaa tagatcaccg ttttttcggc
     8941 cgactggcgt acaaacagaa gagaatggag ctggttgcgc agaatgcaat tgaccaaatc
     9001 atcggccatg cagagttaaa ggtcgcaaga gaaagtaatg cgccaacaat tgcccctatg
     9061 ccgaaacgga aggttcctgc cagcgaagat gccgttcccg ccatatgggg aaactcatca
     9121 agaatgaccg ccatcgcatt ggatgacacc atcgacacgc agcccacaaa cgccgcaacg
     9181 ccaaccacca gcgaccagaa tcccagcccc agcagtgcac tgatgaccat ccacgctgcc
     9241 ataataaact gtatccacaa ccccgagcgg aacatattta acgcgccaat acggcggacg
     9301 aagcggctgt taaagatggt catcacgaac agaaaaagaa tgtttagcgc aaagtaataa
     9361 ccaaagtttt ccggcgcgac gtggttgatt tcaatgtaga caaacggtcc ggcgcttaag
     9421 aatgagaaca tcccggcaaa gctgaaaccg ctggccagca tataactcag gacacgctta
     9481 tgacggaaca gcgccgcaaa gttaccaata gtggtacgaa tgtgaaaagg ctggcgacgc
     9541 tgcggcggta aggtttcttt gatcagaaag aagatcattg ccgaagccag aatcgccgcc
     9601 acggcgagga tccagaagat gtaatgccag ctcagccaca ccagcaccca gccgccaact
     9661 atcggtgcca tcagcggtgc aatggtcgtc accagcatga caaacgacat catccgtgag
     9721 aactcttctt tcgggtagat atcacgcatc aaagcgttga taaccacgct ggccgcagcc
     9781 gcagccagcc cgtggaagaa acgcatcaca atcagctgat cgatggtttg tgccaacgca
     9841 cacgccaccg cggcggcggc aaacaccagc gtaccgccaa gaaccaccgg cttacgcccg
     9901 aagctgtctg ccatcggccc gtagattaac tgccccaacg caaagcccag aatataagta
     9961 ctgagggtca tctgcgtact gcccgccggt acaccaaact gcgctgaaat taccggtagc
    10021 gcaggcagat acatatcaat cgacagcggc atcaacatgg ccagcaggcc aaggataaaa
    10081 acaatagcaa acgacgaatg ctgtcgggtg gtcacaacgg gctcctgaaa gtcattgaaa
    10141 agttagacga cgctggcaat ttcttcttca gttaacggac gatattcacc gggggctaaa
    10201 tcagcatcca gcgtaatgcc gccaatacgt tcacgatgca gctcaaccac atggttaccc
    10261 acggcggcga acatgcgttt cacctgatga taacgccctt cgctgatggt cagacgaacc
    10321 tgcgttgggg taatcacttc cagcaccgcc ggcttagtga gatctttttc gttatgcagc
    10381 tgcacgcctt tagcaaattg ctctgccgta tcgtcagcta cgggtgattc cagcgttacc
    10441 agataggtct tctcgcaatg atggcgcggg gaagtaatgc ggtgcgacca ctgaccatca
    10501 tctgtcatta gtaccagacc ggtggtatcg atatccagac gccctgcagc gtgcagtttc
    10561 cacgctaccg gttcatcaag aaaatagagc accgttgggt gatccgggtc gtccgtggag
    10621 caaacatagc cctgaggctt attgagcatg aagtaacgtg gaccgtgttg ctgcgccagc
    10681 gggttgccat cgtaagcgac atcatgttca gggagcagtt tgaacgctgc attacggacg
    10741 atttcgccat cgacggtgac acgattgccg cggatttcac gcccggcaat agcacggcta
    10801 acgccgagtt gctgtgcgat aaatttatca agtcgcatgt gtgtgatttt gcctgtaaaa
    10861 aacggaggtc gggtaatgac ccgaaaatct gaactgttgt ctgcccagta tagcggtcta
    10921 ataacttccc tcaagggaaa aagatccgtg gcatactatt agcagaataa tctacctacg
    10981 cgagaccatg atttttacac tccgcccata tcagcaagaa gccgtggatg ccacgctcaa
    11041 ccattttcgt cgtcataaaa cccctgccgt aatcgtgctg cccaccggtg caggtaaaag
    11101 cctggtgata gcggaactgg cgcggctggc gcgtggtcgc gtgctggtgc tggcacacgt
    11161 taaagaactg gtggcacaaa accatgcaaa atatcaggcg ctggggctgg aagccgatat
    11221 ttttgccgcc gggctaaagc gcaaagagag ccacggtaaa gtggtatttg gcagcgtgca
    11281 gtcggtcgcc cgtaatcttg atgcctttca gggtgaattt tcgctgttga ttgtcgatga
    11341 atgccaccgt attggtgacg atgaagagag ccagtatcag caaatcctca ctcacctgac
    11401 caaagtgaat ccccacttac gcctgctggg gctgactgcc acgccttttc gactgggcaa
    11461 aggctggatc taccagttcc attatcacgg catggtacgc ggcgatgaga aagccctttt
    11521 ccgtgactgc atttatgagc tgccgctgcg ttatatgatt aaacacggct atctgacgcc
    11581 gccagaacgg ctggatatgc cagtagtgca atacgatttc agccgcttgc aggcacagag
    11641 taacggactg ttcagcgaag ccgatctcaa ccgcgagctg aaaaaacagc aacgcattac
    11701 gccacacatc atcagccaaa tcatggagtt tgctgcaacg cgcaaagggg tgatgatttt
    11761 cgccgcgacg gtcgaacacg caaaagagat tgtgggatta ctgcctgccg aagatgcagc
    11821 actgattact ggcgacacac ccggcgctga gcgcgatgtg ttaattgatg attttaaagc
    11881 ccagcgtttt cgctatctgg tcaacgtcgc ggtactgacc accggatttg acgccccgca
    11941 cgtcgatctt atcgccattc tgcgccctac cgaatcggtg agtctttacc aacaaattgt
    12001 cgggcgcggt ctgcgcctgg cccccggcaa gattgattgc ttaattcttg attatgcggg
    12061 taaccctcac gatctctacg cgccggaagt tggtacaccg aaaggcaaaa gtgacaacgt
    12121 tccggtacag gttttctgcc ctgcctgcgg ttttgccaac accttttggg ggaaaacgac
    12181 tgccgacggg acattgattg aacactttgg tcgtcgctgt cagggatggt ttgaagatga
    12241 cgacggacat cgcgaacagt gtgacttccg tttccgtttt aaaaattgcc cgcaatgtaa
    12301 cgcagaaaat gatattgcca cccgccgttg ccgcgagtgt gacaccattc tggttgaccc
    12361 ggatgatatg ttaaaagcgg cgctacgact gaaagacgcg ctggtattac gctgtagcgg
    12421 catgtctttg cagcatgggc acgacgaaaa aggcgaatgg ttgaaaatca cctattacga
    12481 tgaagacggc gcggatgtga gtgagcgttt ccgtctgcaa acgcccgccc agcgaactac
    12541 cttcgagcag ctttttatcc gcccgcatac gcgcacaccg ggcatcccgc tgcgctggat
    12601 caccgccgcc gatgtcctcg cccagcaagc cttattgcga cacccggatt ttgtcgtcgc
    12661 ccgcatgaaa ggccagtact ggcaagtgcg tgaaaaagtg ttcgattacg aaggtcgttt
    12721 tcgtcgggcg cacgaattac gcggttaatg gtagttttga ttgatgtata aggcgattga
    12781 gtatagaatc tcgcccgctt ttgcatacgc aaagcagatc acttacctgt tgctgggtcg
    12841 cctgtagcag gattaattta agagagaaag aaatgtttac tatcaacgca gaagtacgta
    12901 aagagcaggg taagggtgcg agccgccgcc tgcgtgccgc taacaagttc ccggcaatca
    12961 tctacggtgg caaagaagcg ccgctggctg tcgaactgga tcacgacaaa gtcatgaaca
    13021 tgcaagttaa agctgaattc tacagcgaag ttctgaccat cgttgttgac ggtaaagaaa
    13081 tcaaagttaa agctcaggac gtacagcgtc acccgtacaa accgaagctg ctgcacatcg
    13141 acttcgttcg cgcttaattg ctgaataagt tgtaaaaaac cccgctccgg cggggttttt
    13201 tgtctctgca aattatgcct gattacggta ttgctatttt ttgcaggcca gataaggcgt
    13261 tcacgccgca tctggcattc agaacacaaa accgattaat tgccgccaga cgtccggcgc
    13321 tgcaattgat cgcgcaagtt cggcggcgtg cctttaatgg tcagggtatc ggttgctgga
    13381 tcccagaaga tgcgttcacc taacagcatc gcatcaaagt taatagtcaa gccaccgccg
    13441 ctaccggcaa acttcgtcaa ctgacgcagt gtgctacggt ctgccgggaa gctctcttcc
    13501 agttcgtagc ctttttcagc cgcaaactcc gtgaagctca cttcgctgac gcctgccagc
    13561 tctttcgaca gcgattccag ttcaatctct tcacctgcct gcagctgctc attacagtag
    13621 ctataaacct gctggcgcac gttctgtcgc tctgctttat ccagttgtgc ttcggcagtg
    13681 aaatcatcga ccgcttgcaa cagcccacgg ttttgcgctt tcgcgtttaa cccttcgctg
    13741 gcaccgagga aatccataaa gaaatccgcc actttgcgcc ctactcgccc tttcaggaaa
    13801 gtgagatagc gggtcgactc tggattggtt tcccactcgg ttaaatcgat acgcgcaaca
    13861 atatccgcat gattgatatc aagataatgg gttgggttga tgtcgagatt ttcgttaaca
    13921 cgcatactgc tcaggttgct cagaactgcc accagcagat actccaccgc cagatagcga
    13981 tagtggcaaa acagcacgaa tccaccgtca gcaaaaggat atttcgctaa ttcgtcacgc
    14041 aggcgaccgg ttgccgcgcg gctaaatgcc agaaagtcct cttcgccctg acgctgtaag
    14101 cgcagcgtct gcgccagttc actctcttcg ctaaacaagc cgtaagcttt atttttggcg
    14161 ctatagaccc gatgcagttc tgccaccatc tcaacgacgg tttccgtcgg ttccagcaat
    14221 gaatcgcgca acaccagctc aaggttctgc tcatcacgct tgataagctg gtgcagagca
    14281 atctggttga tatccagact catgataaac tctcctttaa gaccgggcgg tattcaacca
    14341 ccgccggtta agcgacgcaa taaaagatgg cggtcatttt acagaaggcg gcgtaacgag
    14401 aatgatttaa cgcataaaaa agcagaaaaa aaaccgttgc tacggtaata tgttgccctt
    14461 tcatcaacta actgatttcg atttatgcca caaatttccc gctacagtga tgaacaggtt
    14521 gaacaattgc tcgcagagct gctcaacata ctggaaaaac ataaggctcc gaccgacctt
    14581 tccctgatgg tgttaggcaa catggtcact aaccttatca acaccagcat tgccccggcc
    14641 caacgccagg caattgccaa ctcttttgcc cgcgccttac agtcctctat caacgaagac
    14701 aaagcgcact aagggaaaca gataacaggt tatggtaact catcgtcagc gctaccgtga
    14761 aaaagtctcc cagatggtca gttgggggca ctggtttgca ctgttcaata ttctgctttc
    14821 gctcgtcatt ggcagccgtt acctgtttat cgccgactgg ccgacaacgc ttgctggtcg
    14881 catttattcc tacgtaagca ttatcggtca tttcagcttc ctggtgttcg ccacctactt
    14941 gctgatcctc ttcccgctaa cctttatcgt cggctcccag aggctgatga ggtttttgtc
    15001 cgtcattctg gcaacggcgg gaatgacgct attactgatc gatagcgaag tctttactcg
    15061 tttccatctc catcttaatc ccatcgtctg gcagctggtt atcaacccag acgaaaatga
    15121 gatggcgcgc gactggcagc tgatgttcat cagcgtgccg gttattttat tgcttgaact
    15181 ggtgtttgcg acgtggagct ggcaaaagct gcgcagcctg acgcgtcgtc ggcgcttcgc
    15241 gcgcccgctg gccgcattct tatttatcgc ctttatcgcc tcgcatgtgg tttatatctg
    15301 ggccgatgcc aacttctatc gcccgatcac catgcagcgc gctaacctgc cgctttcgta
    15361 cccgatgacg gcacgacgtt ttcttgagaa gcatggcctg cttgatgcgc aggagtatca
    15421 acgccgtctt attgagcaag gcaatccaga cgccgtttcc gttcagtatc cgttaagcga
    15481 actgcgctat cgcgatatgg gcaccgggca gaatgtgctg ttgattactg tcgatggcct
    15541 gaactactca cgcttcgaga agcagatgcc tgcgctggca ggttttgctg agcaaaatat
    15601 ttcgttcacg cgccatatga gctccggcaa cactacagac aacggcatct ttggcctgtt
    15661 ttatggcatc tcgccaagct atatggacgg cattttgtcg acccgtacgc ctgcggcgtt
    15721 aattactgcg cttaatcagc aaggctatca gctggggtta ttctcgtcag atggctttac
    15781 cagcccgctg tatcgccagg cattgttgtc agatttctcg atgccgagtg tacgcaccca
    15841 atccgacgaa cagaccgcca cgcagtggat caactggctg ggccgctacg cacaagaaga
    15901 taaccgctgg ttctcgtggg tctctttcaa tggcacaaac attgacgaca gcaatcagca
    15961 ggcatttgca cggaaatata gccgggcggc aggcaatgtc gatgaccaga tcaaccgcgt
    16021 gctcaatgca ctgcgtgatt ctggcaaact ggacaatacg gtggtgatta tcactgccgg
    16081 gcggggtatt ccgctgagcg aagaggaaga aacctttgac tggtcccacg gtcatctgca
    16141 ggtgccatta gtgattcact ggccaggcac gccggcgcag cgtattaatg cgctgactga
    16201 tcataccgat ctgatgacga cgctgatgca acgcctgcta catgtcagca cacctgccag
    16261 cgaatattcg caaggtcagg atttgttcaa ccctcaacgc cgccattact gggttaccgc
    16321 cgcggataac gatacgctgg caattaccac cccgaaaaag acgctggtgc tgaacaataa
    16381 cggtaaatac cgcacttaca acttacgtgg tgaaagagtg aaagatgaaa aaccacagtt
    16441 aagtctgtta ttgcaagtac tgacagacga gaagcgtttt atcgctaact gattaattat
    16501 aaatcagtta gcaaaatatc ttacttgcaa tcggtgtgga aaacggtagt attagcagcc
    16561 acgagtcggc acgtagcgca gcctggtagc gcaccgtcat ggggtgtcgg gggtcggagg
    16621 ttcaaatcct ctcgtgccga ccaaaaatcc caagaaaaaa ccaaccttca cggttggttt
    16681 ttttatatct gcatttaatt cgataaacag acagcgacac atcacggcct gtttattttc
    16741 tgttatcaga acgtccagac tacacccgcc tgagttgtgc tgctttcttc acgggagaaa
    16801 cgactatcga taccgagcgt caggcccgtc aatgtttgct caaagccagc tcccgcatca
    16861 gtgttcacgt tgttgcggct gttaattgcc gaactccaca tcgccgtatc gtaattaacg
    16921 ccttttacgc taccaagacg cccacgcacg gtgtccagtt ctgcatcaaa taccagcggt
    16981 tgtgctgccg ccatattcag cacatccgtg gttgaagggg gcatttgtac gcgattctct
    17041 gcgagactcc agctatgatt gccatgatcc agcaaggtat attcatacgt actgatatca
    17101 acaacgcctc cggcattgcc caacgtaaat gccgcatcac cgcgcccgtt gttaccagtg
    17161 taaggttatc tcctgctgcc aggctggcac cggtgcccgt cacgaatatt ttgaaatcac
    17221 ctgttgcttt aacctccagt agtcctccct tttcgataac ggcaaagacg gcaatcccat
    17281 ctttttcaac aattaagcgc ccacctttaa tcgtgggatc attcgccgcg ccataaacct
    17341 gtagcactcc attattgatc gtagtctggt ttaccgcgtc acccatttga acaatttgat
    17401 aaccatcatt taatactgag tattctgcgg tgccatttat ttcaacgtac tgatatcccc
    17461 cgttaatttc agtattacta cttacaccaa attcttatat atgctgttct ccgccaggat
    17521 tgattttcgt attattggcg gccccataaa ccagctggat atctttttcc aggacaacac
    17581 catcaactgt ctcaccatac tcaacagttg atgccaatgc tatgggtgaa tataacgctg
    17641 tagccaccag aacagatatt gcggaacgac aaagagaaac agaaccagat tggtgcatta
    17701 agctttcatc ctatgaaatt aattgctgtt aaaagcattg gggacagaaa atacccatag
    17761 ctccataccc ggagtcagtt ttaaaaaact gtttaaagaa atgcacaagt attgtgattg
    17821 attttttagt tgtttttcct gatgagaagc tgatgcaaaa ttccgtcttt ataatgaaga
    17881 tgatgccaaa gcgaacgaca aagttgtagt tttcactaca tgtccataca taaaatgggg
    17941 taacattcac gcgcctggta gcgttaccaa cgctacgctc aaacataatg attctaataa
    18001 aacctcagga gactactatg cctgaagcaa cgccttttca ggtgatgatt gtggatgatc
    18061 atccacttat gcgacgcggt gttcgtcagt tactggagct tgattctggc tttgaagtgg
    18121 tcgccgaagc gggcgacggc gcaagcgcga tcgatctggc gaatagactg gatatcgacg
    18181 tgatcttgct ggatctcaat atgaaaggca tgagtggcct ggataccctc aatgccttgc
    18241 gcagggatgg cgttaccgcg caaattatta tcctgaccgt atcagatgcc tccagcgatg
    18301 tctttgcact gatagacgca ggcgcagacg gttatctgtt gaaagacagc gacccggaag
    18361 tgttactgga agcgattcgt gccggagcga aaggcagcaa agtctttagc gaacgcgtca
    18421 atcagtactt acgtgaacgt gaaatgtttg gcgcggaaga agaccccttc agcgtgctga
    18481 cagaacgcga gctggatgtt ctgcacgagc tggcacaggg gctgtcaaat aaacagattg
    18541 cctcggtgtt gaatatttcc gagcagacag taaaagtcca tattcgcaat ctgctgcgta
    18601 aactcaacgt ccgctcacgc gtggcggcca ccattctgtt cctgcaacaa cgcggggcac
    18661 aataaaaata gcccgatggc atcaccatcg ggccactttt tatttactct cctgcggcga
    18721 caaatgttgc atcgcctgcg cgatactacg ttcgatcacc gcacggcgag tatcgttggc
    18781 aggtaagagt ttcaacatca tctcccacgc ggcaacggct tcgccaaatc gttgctgctc
    18841 aaaggcatta aatgcataca tgcttaacac acggatattg ctatggtccg ttctcaccaa
    18901 ctggcgcagc agttcgccgc cgaggcggtt gtcgttggga tcagatgaac gtgtcaacgc
    18961 ttcagcgtat cccagtgcag catcgctgtt tttcggatcg aggcgatacg cagtggcgta
    19021 tgcatcggtg gcgatactgg cgttacccag cgccatgcca acgcggccca acataatcca
    19081 gccttctata tctcccggat ttttttgcag ttgagtacgc atccccagcg caaggcgcga
    19141 catctcttct tcgttgagcg gatcggcttt cggatccagc gccctgtcca gtaacgccgg
    19201 agcctgtgcc gtggcctgct gccagatttt cacctgctga taattgccag tctggtagta
    19261 gctgacgcca gccacaatta acgccaccac aatacccggc agataaacaa catatccggc
    19321 acgcttacct tccggcacgc tttgttctgg aaacgcttcc ggcaccacgc gtacccgacg
    19381 ccgcgaacgg acgtatatga cccaaccgcc aatgccaata gccactactg gcagcaccca
    19441 cagcagcacg gtcagcggcg ttaacggcgg atcgtaagtg acgaagttgc cgtaacgcgc
    19501 caccatataa tcgacaatct ctttcttact tttgccttcc tgcatcagtt catacacttt
    19561 ctggcgcagg tcggtggcaa tcatcgagtt ggaatcggca atgctgttgt tctggcattt
    19621 cgggcagcgc agttcttcag tgagctgacg gaactgttgt tcctgtgctt catctttaaa
    19681 ctgcaacacg tcgatggtcg ccagcgctga gccggagatc atcagcatca gcacgcccaa
    19741 taaaaacctc attgtgcggc ctccttactg tatttctccc acagtggctt gatctcttct
    19801 tcccagacgc gaggattgag atcgcccgca tggcgatagc gaatgatgcc gttgccgtca
    19861 ataaggaacg tttctggcgc gccatagaca ccgagatcca gccctaacat gccgtcgcca
    19921 tcaaacaggc ttagcgcgta aggattgccc agctctttca gccagctgat tgccttctgg
    19981 cgatcgtctt tatagttcat gccgaccacg cggatgccct gcgcagaaag ctgattcaga
    20041 tattgatgtt ccgcacggca ggtcggacac caggtcgccc agacgttaag cagcaccggt
    20101 ttgccctgag tcagcacatc cgcctgataa aactgccccg gattgtccag tgattcgagg
    20161 cgaaacttcg gcactggctt gccaatgagc gccgattcca gattggtcgg atcatcccct
    20221 tcggcattac gcgccagctg ccacagcagc gccgcggcaa tcgccaggaa gataatcaac
    20281 ggaattaaca ataccttgcg cttcatgcag cctccggcgc agttttctgc gggttcacgc
    20341 gcttacgata gcgaggatca aacagacaca gcagtccgcc caacgccatc atcagcccgc
    20401 ccgcccaaat ccagcgaaca aatggtttgt agtaaagacg cacggcccac gcgccgtttt
    20461 ccagctcttc accgagtgcc gcgtacaggt cacgcgtgat gccaccgtca atcgccgctt
    20521 cggtcatcat cgacccggca gtgttgtaat aacgtttttc tgcatacagc actgtttccg
    20581 gcttgccatc tcgcgttacg ccgatagtcg ccacaccgcc acgccagttc ggaccggtca
    20641 cctctttgac atcacggaag gtgaagcgat attcatgaat atcgacgcta tcgccggact
    20701 tcatgcgcac atcacgctca acgctatagt tctgactaaa agcaatgcca acaattgtca
    20761 ctgccagccc caggtgagcc gccaccatcc cccaataact gaaggtggtt ttcgtgccgc
    20821 gtgaaatacg cagcgcagct tccgcaattg ccagcaccgc aatccagcag gccattgcca
    20881 ggccgagcac cgtcatcgcc acaactttgc tttcgaacag ccacggcaac agcagcgaca
    20941 gcaccagcgt agagatgaag gcgataatca acaaattgcg gattttacgt gggcgatcgc
    21001 gtccccagcg caccagaggg ccgacaccaa gcagtagcgc aaacggcacc atcagccagg
    21061 taaacatggt gttgaagaac ggttcgccaa tcgaaatact gcccagtccc agttgcttgt
    21121 gcaccagcgg cagcaacgtc cccagcaaca ccaccagcat cgcggcgacc agcaaaacgt
    21181 tgttcgctaa cagcaaagat tcccgcgacc acagcgcatt gtttacgcgt gagcgaactt
    21241 tgtgtccacg cgcggcaaac agcagcagcg aaccgccaat caccagcacc ataaaggcga
    21301 ggataaacat accgcgcgcc ggatcagacg caaacgcgtg taccgatacc agcacgccgg
    21361 aacgcaccag gaaagtcccc agcagacaca acgagaaggc actgattgcc agcagtaatg
    21421 tccacgcttt gaagctggcg cgttgttcag tgaccgccag tgagtgcatc agcgcagtcc
    21481 ccaccagcca cggcataaac gaggcgtttt ctaccggatc ccagaaccac cagccgcccc
    21541 agccgagttc gtaataggcc catgcggaac cgagcacaat gccgagcgtc aggaaaatcc
    21601 acgccgccag cgtccacggg cgagtaaaac gcgcataagt gctgtccaga cgcccactca
    21661 gcaaagaagc aatggcaaaa gcaaacgcca ccgagaaacc cacgtacccc atataaagca
    21721 gaggcggatg gaagatcagc cccggatcct gcaacagcgg gttaagatca cgcccttcaa
    21781 tcgggaaatt cggcagcgtg cgtgagaacg ggttagaggt aaagagaatg aacagcaaaa
    21841 agccgacact gaccatcccc attatcgcca gtacgcgggc aacaatatcc agcggaatac
    21901 gctgactaaa aatcgctacc gcaaaggtcc agccgctcat cagtagcacc cacagcagga
    21961 gcgagccttc atgcgcgccc caggtagccg ccacgcgata ccacaccgga agctgggtat
    22021 tggagttgct ggcaacatag gtgacggtga agtcgttgac cacgaaggca ttgaccagta
    22081 ccagaaatgc gccagccaca gacataaaca gcagccaggc aaacaagcgg gaagacgcca
    22141 tcatgcgcgc atctccgcgc gctacgcccc atagcggata cccggacagc agcagcgcaa
    22201 ttcctagcgc cagacacagc agcccgttac caatttccgg catcatgatg ctgggtcctt
    22261 ataaacactc gccgggcgac ggtgattggc ttccatcgct ttctcaactt ctggcggcgt
    22321 gtagttttca tcgtgtttcg ccagcacttc tttcgcgagg atatgattgc ctttttccag
    22381 ctcgccctgc accacaacgc cctgcccttc acggaacaga tccggcaaaa tgccttcgta
    22441 agagacatcc actgagcctt cagcgtcgta aatggtgaag gtcactttca gcgaattggg
    22501 atcgcgctgc acactacccg gcatcaccat cccgccaacg cgcagacgct gaccgacttc
    22561 cggcatttgc tgagtttcac gcttgccgta gagaatttcc cccggcgtat aaaagagatc
    22621 gatattcgag cgcagcgcat atagcaccag accgatagtc agcgccagcc ctgccaacac
    22681 ggcacaggca atccacaagc ggtttttacg gcgaatattc atgcagcctc ctgctgttgc
    22741 gcagcacgta aacgcgcctc acgcgcccgc tgttgcgcca cgccacgcag aattgcgcga
    22801 tgttgcatca ccgagtgcac gaccaaaacc accagcggaa taacggtcat caccaccgcc
    22861 agccagacaa aaaaggcgta accgcccatt gcgaaaaatt cattccagga agcaaatgca
    22921 ggggtcattt acggcctctt ttcagtatca gttcactcac ccacggacgg cgtttttcca
    22981 tcagcaaaat caaattacgc atccgcatca gcgtcagcgt ggcagacagc agcaggaaac
    23041 caaaaatcga ccagcgcagc ggcgaacgca tcgccggatc aatactttgc tgcatccgcg
    23101 ttgatccctg atgcagggtg ttccaccact ccacggagta atgaataatc ggcaggttca
    23161 ccacgccaat cagcaccagg atacctgccg cacggccagc cagacgacgg tcgtcgaagg
    23221 cgtgccacag ggcaatgaca ccaacataca gaaacagcag caccagttca gaagtcagac
    23281 gtgcatccca tacccaccag gtgccccaca tcggttttcc ccatgcagag ccggtaacca
    23341 gggcaataaa ggtaaacacg gcaccaatgg gggccatcgc cgccaccgcc aggttggcca
    23401 ttttcatctg ccagaccagg ccaataaacg ctgccaccgc cattgatgca taaatgccca
    23461 tcgaccagat cgccgcaggc acatgcaggt agataatgcg gtagctattt ccctgctgat
    23521 aatcagccgg agcaaagccg aacccccaga tccagccgac ggtaagcacg accacactgg
    23581 caattgccag ccacggtata aaccagccac agatttgata cagccgtggt gggatcgcca
    23641 gttgatgcag tgttttccac atagtttcga taccagactc gaacaaaaat cagtaatcca
    23701 gcgttattga atgctgattc gtaacgctgc cgccgtcgca aaaggactta atgtcgcggt
    23761 gcctgccagc aacgcgccta aaattgccag atacccatca acgggcaaat gcatagaagc
    23821 cgcgtccatc gcggcggtgg caaagatgag taatgggata gtcagcggta acaccagtat
    23881 gctgagcagc acaccaccgc gcttaagtcc cactgtcagc gccacgcccg gcgcaccaag
    23941 aaagccaagc gtaggggttc ccagcagtag cgttaacgcc atcacttgcc agccataaac
    24001 atccattccc agtagcattg ctaccagggg cgaaaggatg agtaacggca gaccggttac
    24061 catccagtgc gccatcacct tcgccagcac agcggcgggc aagggtaacg gcaacaacat
    24121 caattgttca agactgccgt cctgcaaatc gtcacggaac agtcgctcga gtgccagcaa
    24181 ggatgaaagc agcgcggcaa cccagataat gcccggcgca atacgcgcca gcagttgcgg
    24241 ctccggaccg atactgagcg gaaaaagggt aattacaatc aggaagaacc acagcgggtt
    24301 ggcgatttcg gcgctatggc gaaacgctac gcgcagctca agacggaaaa tgcgccagaa
    24361 catcatgcgg cccccgtttg cgtcagtgaa atgcggcgaa ttttactttc agcaacgttg
    24421 agcggctggt gggtagtcag aatcacaatc cccccctgct ccgtatgctg cgccatacgc
    24481 tgggtcagac gatcgacgcc gttaacgtca atcgcggtaa aaggctcgtc gagtatccat
    24541 aacgtggcac gggtcagcca cagacgcgct aaagcgacgc ggcgttgttg cccggcagaa
    24601 agttgattta caggaatatc ttcgaatccg gcaagcccgg cctgcgccag ggcttccaga
    24661 cattgtgcgg tatcgccatc gcgatgataa aagtgcagat tttctaacgc cgtcagccgg
    24721 gttttgatcc ccggctgatg gcctatccat aacaggtttt gatggtagct gtcgcgtacc
    24781 tgatgcaagg gctgcccttg ccagagaacc tcgcctgcgt cagggcgaga caaccccgtc
    24841 agcaaacgga gaagcgttgt cttccccgcg ccgttgctac cggtgatttg tacccactct
    24901 cctgcgttca gcgtaaatga taaaccacta aataaggttc gttcatcccg ctcgcaaagt
    24961 aactctctgg cttcaagcat acccacgcaa taaccctgtt aaaaacctgg ctcgacttca
    25021 cgcatgtcgg gcagcttgtg cgctatccct ttatggcaat caatacaggt ttgcccatct
    25081 ttcaccgcct ggtcatgcat cttcgcggca accgatttct gggcggttgt atccatatac
    25141 tcgaagttgt gacagttacg gcactcctgc gagttgttgt ccttcatgcg ccgccactca
    25201 ttctgtgcca tcgtcaggcg atgagcttca aatttctgcg gcgtgtcaat aacgccaaaa
    25261 attttaccat acagctcttt acttgctttg agcttgcgta tcatctttgg cacaaactcg
    25321 tgcggaacgt gacaatccgg acaggtcgca cggacgccgc tacggttgtt gtagtgcacg
    25381 gaatccatgt attcctgata caccgtgtta cgcatttcgt ggcagctaat gcagaactct
    25441 tcggtattgg ctttttccat cccggtgtta aagccgcccc agaagacgat gccgccgaca
    25501 aaaccgatca acagcagcgt ccctagcgcc agacggctgg gggtacgcca ccatttccac
    25561 aggcgcttaa tcagaccagg cttacggtca gaatttccca taataacctc ttatttcccg
    25621 taaccttttg atggggtaaa ggtattcccc acgattggcg cggtatcggc ctgcggtacg
    25681 tgacattgca gacagaaata acgacgcgga gccacttccg cgcccacttt gccgtcgctg
    25741 tccataaagt gagtaggact aatgcgcggc gcgccagtgg tgcgataact ttcgacaccg
    25801 tggcactgca agcagcggtt ggtattggtc gttacctgat aaccttcaac gctatgcggg
    25861 atcattggcg gctgattcac atagttcagc ggcatccgat cctgctcttt tggcatccga
    25921 atggcccctt cctgcgtccc ggatacttcc ggcgactggc taaaatcgac gccattagcc
    25981 gcccaaaccg cgccgcttac caccagggcc agcatcgccg tccattgaca cagcgctttc
    26041 ttcaggtcat ggcttttcat gatttcgctc ccgaactcca tcgtgtagtt attgtaaata
    26101 catcctcaga acagacatcc acgcagcgac cgcacgtcat gcaatcgcgg ctggtgacct
    26161 gcaccgggct ttgttcatcc agcaccgggg cacgtagcac atgcggttcc gggcaaacat
    26221 gaaaacaatc catacagcgg ttacatttct gccgatcggt ggccgcaacg gtaatcacac
    26281 ctttgctacc cagcacgcca tacagcgcgc caaccggaca aatgtgcccg caccagccgt
    26341 gttcaacgac cagtagatca aataaaaaca gcgcgagaat aagcagcgcg ccgctgccga
    26401 agcccattac caggctacgc cccatcaggg aaacggggtt gatccattcc cagatgagcg
    26461 tgccagttaa ggccgatccc accagaatga ccaccaacag cacgtagcga atatggcgag
    26521 ggatcgtcgc agactgattg aggtcaaatc gtctgcgtaa ccaatttgct aagtcggtaa
    26581 tcgggttcag cggacagacc cagctgcaaa ataatcgctt tcctgccagg gcgtagagca
    26641 cggtgataat caccgcaccg gtcaacgcca ccgtggcagg cagatgaccg ctggcaagac
    26701 tttgcagtgt catcagcgga tcggtcagtg gaacggtgtc gaataacagg ctactgctgt
    26761 agttgccgtg caagatccac acaccaaacc acggaccgct caaaaacatc cccagcacga
    26821 agaactgaca aaggcgacgt aacactaacc aacggtgact gcgccaccag ccttttttct
    26881 ccagcgcctc gcgcccggcg tcacgtttac gatttgccat tgttcccctc cagccagccg
    26941 aagcggtaat ggtgacctaa ctcccctttc gccagtgaca gcggtaacac cttgattgcc
    27001 ggttgttcca gcacgcacac tttttcgcac ttaccgcagc cggtacaggc gtcgctgtga
    27061 accgtcggca gaaagcgggc gtgcttagcg gtacgcgtgt tgcgctccag ctccagggtg
    27121 atggcctcat caattttcgg gcattcgcga taacaaacat cgcagcgcag cccctgaaag
    27181 ttgagacagt tttcctggtc caccagtacc gccagcccca tccgcgcgtc gtcgatcgat
    27241 tcgatctcac gatccagcgc accgctcggg cacactttgg cgcacggaat gtcctcacac
    27301 atttcgcaag gaatatcccg tgcgacaaaa tatggcgtgc ccgccgacag accagaggcc
    27361 agggtcgcca gttttaaggt gtcgtaaggg caagcctgaa cacactgacc gcaacgcaca
    27421 caggcactgg caaaggcgtt ctcgtttatg gctccgggcg ggcgcaaccg cacaccagat
    27481 gcgcgtgcgg tttgctgttg taaccccagc gccacaccca cggcagccag cccgcctgct
    27541 gtgcgaacga catcgcgcag aaagcggcgg cgaccatttt gaggtttcgc tgaccgggac
    27601 ataatggctt acaccttctc cagtttgacc gcgcacttct tgaagtccgt ctctttcgag
    27661 agcggatcgg tcgcatccag cgtcagtttg ttaaccagct gtgcggcgtc gaagaacggc
    27721 atgtacacca ggccctgcgg cggacggtta cgaccgcgcg tttcgacaat cgagatcact
    27781 tcgccacggc gagaaaccac tttcactttg tcgccacggc gcagatcgcg cgctttcgca
    27841 tccagcgggt gaataaacag aaccgcttcc gggaaggcgc ggtgcagttc cggtacacgg
    27901 cgagtcatac tgccggtatg ccagtgctcc aggacgcgtc cggtagagag ccacaggtcg
    27961 tactcttcat ccggtgcttc cgccgcaggt tcgaacggca gcgcgaagat caccgctttg
    28021 ccatccggtt taccgtagaa cttgtagcct tcgcccgctt tcacgtacgg gtcgttacct
    28081 tcgctgtaac gccactgcgt ttctttaccg ttcaccaccg gccagcgcag accgcgtgct
    28141 ttgtggtaat catcgaacgg cgcgagatcg tgaccgtgac cgcgaccaaa ccatgcgtac
    28201 tcttcgaaca gccctttttg cagatagaag cccagctcgc gggattcatc gttcagctga
    28261 tcttccgcca gttcggatac cgggaatttg ctcacttccg gagtggcata cagaacgtcg
    28321 tacagcgttt tgccgcgcag ttctggtttc ttcgccagca actcttccgg ccacacttct
    28381 tcagttttga agcgacggga gaactgaact aactgccaga gatccgattt tgcttcaccc
    28441 ggtgcctgta cctgctgacg ccagaactga gtacggcgtt cggcattacc gtaagcgccc
    28501 tctttctcta cccacattgc ggtcggcagg atcaagtcgg ctgccagcgc actgaccgtc
    28561 ggatacggat cggaaacgat gataaagttg cgcggatcgc gccagcccgg catacgctct
    28621 tcattaatgt tcggtccggc ctgcatgtta ttggtacaca tggtccagta aacattcagt
    28681 ttgccgtctt tcagcgcacg gtcttgtgcc accgcgtgca gaccgatttt cgccggaatg
    28741 gtgccactcg ggatattcca cttcttctcg caaatatcac gatgtttctc gttagtcacc
    28801 accatgtccg caggcagacg gtgagcaaag gtgcccactt cacgcgcagt accacacgcg
    28861 gaaggctgcc cggtcaggga gaacggaccg caacccggct gggaaatttt gccggtcagc
    28921 aggtgcaggt tgtagaccag gttgttggcc cacacaccac gggtatgctg gttgaagccc
    28981 atcgtccagt aggagatgac tttcttgttc ggatcggcat acagctgcgc cagttgttct
    29041 aactggtctt tcggcacgcc ggtcatttcg gcggtttttt ccagcgtata ttcagcaacg
    29101 aaggctttgt aatcttcaaa gctcatcggt tcggaggcgt cagaacccgg attcttcgct
    29161 gctttttcca gcggatgggt cggacgtaaa ccgtagccga tgtccgtcgc ccctttgcgc
    29221 aggttaacgt gtttgctgaa gaagtcctga tttatcgcat tgttttgaat gatatagttg
    29281 gcgatatagt tcaggatcac caggtcagat tgcggcgtaa agatgatgcc gttatccgcc
    29341 agctcgaagc tacgatgctg gtaggtagaa agcaccgcca cggtgacgtt ctggttagaa
    29401 agacgacggt tagtgatgcg cgaccagagg atcgggtgca tctccgccat gttggagccc
    29461 cacagcacaa acgcgtcagc ctgctcgatg tcgtcatagc agcccatcgg ctcatccata
    29521 ccaaaggtac gcataaagcc aactactgcc gacgccatac agtgacgcgc gttcgggtcg
    29581 atgttgttcg aacggaagcc cgctttgaac aacttggacg cggcataacc ttcccagata
    29641 gtccactgac cagaaccgaa cataccgatc gattccggcc ctttttcttt cagggcggtt
    29701 ttgaatttat cttccatcac atcgaaggcc tgatcccagg tgattggggt aaattcgcct
    29761 tctttgtcat atttaccgtt tttcatacgc agcagcggct gcgtcaaacg gtctttaccg
    29821 tacatgattt tgggcaggaa atagccctta atgcagttca ggccacggtt aaccggtgcg
    29881 tccgggtcgc cctgacaggc caccacacgc ccctgctgcg ttccgaccag aacgccgcaa
    29941 cccgtaccgc agaaacggca cggcgcttta tcccatttaa tggcttcctg ctgaccaaca
    30001 acggcgcggg caacgcccgg cacgctgaga ccggcagccg ccgcagcggc cgcaacggcg
    30061 ttagctttca taaagctacg acgactgagt ttcatggtgt ttcctcacct tgctcttcct
    30121 gctggtgata aaccagcgac accgccagca cgccctctac gttgcgtact gactcaatgg
    30181 tttggatcag cgtttcgctg tcttctgctt ccaccaccac aatcaactga ccgctcggcg
    30241 cgtcgctgac agcaacttca cagccggtaa aggcgttcag ttgggtgctg atgtctgaaa
    30301 ttcgttcgct tttggcctgc acgaccaggc tgcaaacttg ccagttagtg tgcatggaga
    30361 tactccgcag ttatggctga taccggacag ctggctgcac atgctccaca tccgttgcaa
    30421 agttggctat taagttgcgg ctggtagatc ccggagagcg tcgggcgaaa gataattgcc
    30481 attggctcac agctatcctg acagcggcgg cattcaactg actgatacgc caggcacgcg
    30541 tccccaatgg tgaactgcaa atcccaggcc ctggtgtggc gcggagaaaa tagcgattcg
    30601 gggcaagcct gcgcgcaggc gtaacagaag ctgcactcgt tatttttgaa attaacgctc
    30661 ggatagccac ccgcgccgcg ttgcagaatg ttgttttcac aagcattaat acaggcgtcg
    30721 cagcgggtgc aatgggtcag aaaatgagat tcatcaccgg accagggcgg acggataccg
    30781 ttactggctt tgcgccagcg accagtgagt atgccccgac gggatgcatc aatcttcaca
    30841 ttgaccttcc atcattaacg cgttctgaaa attgagagcg accaaataaa ccgcataatt
    30901 aataagccat ttttatagcc gctaagatat taaaggatgt gtcaaagatg cataccccga
    30961 tcggggtaaa acctgtagca ggatcaaaag agtggcggga agcgtggcaa aaacgg
//
LOCUS       DAZFJL010000045        30769 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFJL010000045 DAZFJL010000000
VERSION     DAZFJL010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.045"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(72..2519)
                     /gene="glgP"
                     /locus_tag="ACHJ8C_002003"
     CDS             complement(72..2519)
                     /gene="glgP"
                     /locus_tag="ACHJ8C_002003"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO5551862.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNDLQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2538..3971)
                     /gene="glgA"
                     /locus_tag="ACHJ8C_002004"
     CDS             complement(2538..3971)
                     /gene="glgA"
                     /locus_tag="ACHJ8C_002004"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO5551863.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3971..5266)
                     /gene="glgC"
                     /locus_tag="ACHJ8C_002005"
     CDS             complement(3971..5266)
                     /gene="glgC"
                     /locus_tag="ACHJ8C_002005"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO5551864.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5284..7257)
                     /gene="glgX"
                     /locus_tag="ACHJ8C_002006"
     CDS             complement(5284..7257)
                     /gene="glgX"
                     /locus_tag="ACHJ8C_002006"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO5551865.1"
                     /translation="MTQLAIGKPTPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIYG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAIMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNDRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALME
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7254..9440)
                     /gene="glgB"
                     /locus_tag="ACHJ8C_002007"
     CDS             complement(7254..9440)
                     /gene="glgB"
                     /locus_tag="ACHJ8C_002007"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO5551866.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWAIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNHTYCHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGAVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9713..10816)
                     /gene="asd"
                     /locus_tag="ACHJ8C_002008"
     CDS             complement(9713..10816)
                     /gene="asd"
                     /locus_tag="ACHJ8C_002008"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO5551867.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELANPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            11008..11601
                     /gene="yhgN"
                     /locus_tag="ACHJ8C_002009"
     CDS             11008..11601
                     /gene="yhgN"
                     /locus_tag="ACHJ8C_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO5551868.1"
                     /translation="MNEIISATVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11658..12998)
                     /gene="gntU"
                     /locus_tag="ACHJ8C_002010"
     CDS             complement(11658..12998)
                     /gene="gntU"
                     /locus_tag="ACHJ8C_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO5551869.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRVH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(13002..13529)
                     /gene="gntK"
                     /locus_tag="ACHJ8C_002011"
     CDS             complement(13002..13529)
                     /gene="gntK"
                     /locus_tag="ACHJ8C_002011"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO5551870.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RCNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13668..14663)
                     /gene="gntR"
                     /locus_tag="ACHJ8C_002012"
     CDS             complement(13668..14663)
                     /gene="gntR"
                     /locus_tag="ACHJ8C_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO5551871.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGILLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14756..15451)
                     /gene="yhhW"
                     /locus_tag="ACHJ8C_002013"
     CDS             complement(14756..15451)
                     /gene="yhhW"
                     /locus_tag="ACHJ8C_002013"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO5551872.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALVKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEILLFDLPPV"
     gene            complement(15574..16611)
                     /locus_tag="ACHJ8C_002014"
     CDS             complement(15574..16611)
                     /locus_tag="ACHJ8C_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO5551873.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTI
                     AQAKELFALAKSKGLIVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            16945..17433
                     /gene="yhhY"
                     /locus_tag="ACHJ8C_002015"
     CDS             16945..17433
                     /gene="yhhY"
                     /locus_tag="ACHJ8C_002015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO5551874.1"
                     /translation="MSEIVIRHAETRDHEAIRQIHAQPEVYYNTLQVPHPSEQMWLER
                     LTARPGIKQLVACIDGIVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17494..19227)
                     /gene="ggt"
                     /locus_tag="ACHJ8C_002016"
     CDS             complement(17494..19227)
                     /gene="ggt"
                     /locus_tag="ACHJ8C_002016"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO5551875.1"
                     /translation="MIKPTFLRRVAIAALLTGSCFSTVAAPPVSYGVEEDVFHPVRAK
                     QGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLISKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMQKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTIKLLEAKGQKVALKEAMGSTQSIMIGPDGELYGASD
                     PRSVDDLTAGY"
     gene            19347..19787
                     /locus_tag="ACHJ8C_002017"
     CDS             19347..19787
                     /locus_tag="ACHJ8C_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO5551876.1"
                     /translation="MKRLLLLTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19774..20517)
                     /gene="ugpQ"
                     /locus_tag="ACHJ8C_002018"
     CDS             complement(19774..20517)
                     /gene="ugpQ"
                     /locus_tag="ACHJ8C_002018"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO5551877.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGARYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKTVALAARELWTGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20514..21584)
                     /gene="ugpC"
                     /locus_tag="ACHJ8C_002019"
     CDS             complement(20514..21584)
                     /gene="ugpC"
                     /locus_tag="ACHJ8C_002019"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO5551878.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRSMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLAGRVNNEGTHFELDGGITLLLNGGYRQYAGRKM
                     TLGIRPEHIALSSRAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            complement(21586..22431)
                     /gene="ugpE"
                     /locus_tag="ACHJ8C_002020"
     CDS             complement(21586..22431)
                     /gene="ugpE"
                     /locus_tag="ACHJ8C_002020"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO5551879.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQEVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELMEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22428..23315)
                     /gene="ugpA"
                     /locus_tag="ACHJ8C_002021"
     CDS             complement(22428..23315)
                     /gene="ugpA"
                     /locus_tag="ACHJ8C_002021"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO5551880.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDAFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23413..24729)
                     /gene="ugpB"
                     /locus_tag="ACHJ8C_002022"
     CDS             complement(23413..24729)
                     /gene="ugpB"
                     /locus_tag="ACHJ8C_002022"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO5551881.1"
                     /translation="MKPLRYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADIATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(24938..25315)
                     /locus_tag="ACHJ8C_002023"
     CDS             complement(24938..25315)
                     /locus_tag="ACHJ8C_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFO5551882.1"
                     /translation="MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNK
                     YEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALFITLLFLKRNGLILAANPEFVEL
                     TVDVAAGKVSLEDIVTRLRQHGT"
     gene            complement(25312..25533)
                     /locus_tag="ACHJ8C_002024"
     CDS             complement(25312..25533)
                     /locus_tag="ACHJ8C_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO5551883.1"
                     /translation="MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEF
                     ERYQKAKLDAEFDAIMAIHGSEIMELADK"
     gene            complement(25621..26334)
                     /gene="livF"
                     /locus_tag="ACHJ8C_002025"
     CDS             complement(25621..26334)
                     /gene="livF"
                     /locus_tag="ACHJ8C_002025"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO5551884.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(26336..27103)
                     /gene="livG"
                     /locus_tag="ACHJ8C_002026"
     CDS             complement(26336..27103)
                     /gene="livG"
                     /locus_tag="ACHJ8C_002026"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO5551885.1"
                     /translation="MSQTLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(27100..28377)
                     /gene="livM"
                     /locus_tag="ACHJ8C_002027"
     CDS             complement(27100..28377)
                     /gene="livM"
                     /locus_tag="ACHJ8C_002027"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO5551886.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASEIR
                     WQWVFIGTAVVFFFQLVRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(28374..29300)
                     /gene="livH"
                     /locus_tag="ACHJ8C_002028"
     CDS             complement(28374..29300)
                     /gene="livH"
                     /locus_tag="ACHJ8C_002028"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO5551887.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMLGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(29348..30457)
                     /gene="livK"
                     /locus_tag="ACHJ8C_002029"
     CDS             complement(29348..30457)
                     /gene="livK"
                     /locus_tag="ACHJ8C_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO5551888.1"
                     /translation="MKRNAKTIIAGMIALTISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPTNQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
BASE COUNT         7450 a         8557 c         7699 g         7063 t
ORIGIN      
        1 tgtatagaaa taccgtgagt tccactctgt attacttcag cggaacacac ggtatttagc
       61 cgtatttgaa cttacaatct caccggatca atatgccaga tatgatcggc gtactctttg
      121 atcgtacggt cagaagagaa gtagcccata ttggcaatgt tcagcatcgc ttttgcggtc
      181 cactcttcct gacgctcgta gagttcgtcg actttatcct gacaatcgac ataactgcga
      241 taatccgcca gtacctggta gtgatcgccg aagttgatca gcgaatcaac cagatcgcga
      301 tagcgacccg gatcttccgg actgaataca ccgctgccga tttgcgtcag cacctgatgc
      361 agctcctcat ctttctcgta gtattcacgc ggtttgtagc cctgacgacg cagttcttcc
      421 acttcttccg ctgtgttacc aaagataaag atattgtcag caccgacatg atccagcatc
      481 tcgacattcg caccgtccag cgtaccgata gtcagcgcgc cgttaagcgc aaacttcatg
      541 ttactggtgc cggaagcttc cgtccccgcc agcgaaatct gttcagacag atcggccgcc
      601 ggaatgatca actgcgccag gctaacactg tagttcggga tgaacacgac tttcagcttg
      661 tcgccaatct gcggatcgtt gttgatcact ttcgctacgt cattgatcaa atgaataatg
      721 tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa aaatattcac gcgcggtacc
      781 cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg tgatcacgtg caacacattc
      841 atcaattgac gtttgtattc gtgaatacgt ttgatttgca catcgaacag cgctttcgga
      901 tttaccacca cattcagctg ctgggcgata tactccgcca gacgcttttt gttctccagc
      961 ttcgcctgat gcaccgcgtg attaaccatt gggaaatcac agtgttgttg cagatcatta
     1021 agcaggctaa ggtcggtgcg ccagttgcgg cccaggtgtt cgtccaacac ggctgaaagc
     1081 gatgggttcg ctaccgccaa ccagcgacgc ggcgtcacac cgttggtgac gttggtgaaa
     1141 cgacccggga agattttcgc aaagtcggca aatagcgatt gcaccatcag gttagagtgc
     1201 agctccgata caccgttaac tttgtggctc accacaaccg ccagccaggc catacgcaca
     1261 cgacgaccgt tggattcatc aatgatcgac gcccgtccca gcagatcggt atcgttcgga
     1321 tactgttcct gcaaggtttt caggaaatag tcgttgattt caaagatgat ctgcaggtga
     1381 cgcggcagaa ttttacccag catatcgact ggccaggttt ccagcgcctc gctcattagc
     1441 gtgtggttgg tgtaggagaa gacctgacaa cacacttcaa acgcgtcgtc ccagctaaat
     1501 tggtgctcat cgatcagcag acgcatcatc tcaggaatcg acagcaccgg atgagtatcg
     1561 ttgaggtgaa tcgcaatttt atccgccagg ttatcgtagg ttttatgcaa ctgataatgg
     1621 cggcttaaaa tgtcctgaat ggtcgaggaa accaggaagt attcctgacg cagtcgtagc
     1681 tcacgcccgg agtaggtgga gtcgtccgga tacagtacgc gagatacgtt ctcggagtgg
     1741 tttttatctt ccactgccgc gaagtagtcg ccctggttga atttaccgag gttaatttcg
     1801 ctactggctt gggcactcca caaacgcaac gtgttggtcg cgtcggtgtc gtaacctggg
     1861 attatctgat cgtaagctac tcccagaatc tcttcggttt caatccagcg cgttttttta
     1921 ccttcctgct gaatgcgacc gccaaaacgg actttatagc gcgtgttgtg gcgtttgaat
     1981 tcccacgggt taccgtattc cagccagtag tcaggcgact ctttctggct accgttaacg
     2041 atgttctgct tgaacatacc gtagtcatag cggatgccgt aaccgcgccc cggcaaccct
     2101 aacgtcgcca gagaatcgag gaagcaagcc gccagacggc ccagaccacc gttaccgagg
     2161 cccgggtcat tttcttcatc aatcagctct tcgagattta atcccatcgc ttccagtgcg
     2221 ccctgtacat cttcgtaaat tcctagcgac aacatggcgt tggaaagcgt acggccaatc
     2281 aaaaactcca tcgacaggta gtaaacctga cgagtttctt gcgacaactg ggcacggttt
     2341 gagcgtaacc agcgctccac gagacgatcg cgcacagcaa ataacgttgc gttcagccat
     2401 tcatgtttat tggcgacgac cggatccttg ccaatcgtaa acatcagctt gtaagcgata
     2461 gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat atgtaaacgg agcattcata
     2521 taggcgtttc ctgaaaacta tttcaagcga tagtaaagct cacggtacga cttcgccgcg
     2581 acctgccagc taaaatccat tgccatagcc tgacgttgca caaaccgcca cagtgaagga
     2641 cgggaccaca gtacaaaagc acgtcgaata gcccgtaaca gcgaccaggc attactatct
     2701 tcaaagacaa acccactggc gacgccatct gcaaggttct caagagaaca gtcagaaacc
     2761 gtatcagcaa gcccaccggt gcgccgcact aacggcagcg taccgtactt caatccataa
     2821 agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca gaatgacgtc cgcgccgccc
     2881 ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa cgcccacctg gccggggtat
     2941 tccgctgccg ccgcaaggaa accttcctgc agcaccggat cgcccgcgcc gagtagcgcc
     3001 agctgcccgc cctgctccag aagaccaggt aaggcttcca gcaccaggtc gagacctttc
     3061 tggctggtca gacggctcac cactgcaaaa agcggcactt tatcgtcaac cttaagcccc
     3121 attgcgattt gtaactggcg cttattttcc gctttatctt ccaacgtatc gcgggtgtaa
     3181 cgcgaggcca acagtaagtc cgtctctgga ctccagattt tctcgtccac gccgttcagt
     3241 acgccggaaa gacgcccttc acggtgacgc tgttgcaaca gaccttccat accgtaggca
     3301 aactgcggtt cggtgatctc gcgagcgtag gttgggctga ctgccgtaat gtgatcggca
     3361 tagtacagac cggccttcag gaaagagatt tgtccgttga attccagccc atgaatatta
     3421 aagaatgacc atggcaattg gatgtcattc atgtgatgtg cataaaacat gccttgatag
     3481 gccaagttgt gcacagtaaa caccgacttc gccggacgcc cgcgcgccgc cagatacgca
     3541 ggcgcaaggc ctgcatgcca gtcgtgcgca tgcaccacat caggacgcca gaatgggtca
     3601 agcccgctgg ccatttctgc cccaacccac cccagcagcg caaaacgcaa tacgttgtcg
     3661 gtataggcaa ataagttggt atcgtgatac gggcttcccg gacgatcata gagatgcggc
     3721 gcgtcaatca ggtatatgcc aaccccgttg taatgaccga acaaaagcgt gatatgtccg
     3781 gcgaaggtat cacgacggga tactacctgc gcatcggtca cgccacggcg aatatcggga
     3841 aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga tttgtgctgc gggtaatgcc
     3901 ccaataacat cagccagacc gccggtttta agcagcggga acatctctga acatacatgt
     3961 aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg tagcatttcg cgcgttacca
     4021 gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc ttcctctgcg ttttcaccaa
     4081 tcaccatgcc ttccggaata acacaagcac gatcgatgac gcagcggcgc agacggcacg
     4141 agcgacctac ccatacttcc ggtaacaata cggcggaatc aatgttgcag aatgaattca
     4201 cgcgaacgcg cgagaacaga acggactgca ccaccaccga accggagatc acacaaccgc
     4261 cggataccag tgagttaagg gtcatcccgt ggctaccgga gcgatcctgc acgaatttcg
     4321 ctggcggtaa tgattcattg taggtgcgaa ttggccaatt gcgatcgtac atatccagtt
     4381 ccggcaccac agaggccaga tcgaggttcg ctttccagta agcttccagc gtacccacat
     4441 cgcgccagta cggctcggca tccgggtcgg attgtacgca agagagcggg aacgggtgcg
     4501 cataggccag accggcttcg gtgatcttgg gaatcaaatc tttgccaaag tcgtggctgg
     4561 agttctcatc gcgatcgtct tcttccagca gttcatacag atagtcggcg tcaaagacgt
     4621 agatacccat actcgccaga gatttgctcg gatcgttcgg cattgacggc gggttagctg
     4681 gtttttcaac gaattcgata attttatcgt tctcatcaac cgccataacg ccaaacgcgg
     4741 aggcttcttc aatcggtact ggcatacaag caacggtgca acgtgcgcct ttttcgacgt
     4801 ggtcgataag catacgcgag tagtcttgct tgtagatatg gtcgcccgcc aggatcacca
     4861 cgtattccgc tttataacgg cggataatgt cgaggttttg ggtgaccgca tctgcggtgc
     4921 cgcgatacca gttttcccct ttcattctct gctgtgctgg cagcagatcg acaaactcgt
     4981 tcatttcttc attgaagaat gaccagccgc gctggatgtg ctgcaccaga gtgtgggact
     5041 ggtactgggt gatcacgccc atacgacgga tcccggagtt gatgcagtta gacagcgcaa
     5101 agtcgataat gcggaactta ccgccgaagt gtacggccgg ttttgctcgc ttattggtta
     5161 aatccttcag gcgggtacca cgtcctcccg ccagtatcag ggcaacagat ttcaatggca
     5221 gctggcgcgc caacattaag tgatcgttct tctctaaact aaccatgact aactcctttt
     5281 ttatcatctc tggaacacac acaatccgtg tgcaggtccc tgccagacag ccgtaatcac
     5341 tgggttatcc tctccagcga atgggggaat ggcgtgccac tccccagcag gtaaaacaat
     5401 ctctgttacc tcaagcgtgg cattaattgc gatcaaaaag cgatccgaga gcagaatttg
     5461 cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa ggttgagcat atcgatttag
     5521 ccaacggaca ttgccgtcgc cttcttccca ccagcgattc tccatcaaag cgggaatgcg
     5581 cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa ccactgcttg cctgcgacca
     5641 gtccaaccag gttaattggt tatcctgaca gtaggcattg ttattgccat gctggctgtg
     5701 accatgttcg tcaccggcca gtaacatcgg cgtaccctgg gagagcaaca acgttgttaa
     5761 cagggcgtga atgctgtcgc gccgccgttc aaccagatca agagtaccgc ctaacccttc
     5821 tttaccatga ttgttactgt aattgttgtt ggtcccgtcg cgattttctt ctccgtttgc
     5881 ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc gtaaaaccgt catgcgcggt
     5941 gacgagatta atcgcggcac tcggcagacg atcattacgt ttaaaaacat cgctggaggc
     6001 agcaaaacgc ccggcaaacg ctcccagagg taaatcataa tgcagccaga aacgacgggc
     6061 tgcatcgcgg aaatgatcgt tccactcggc aaacagcggc gggaaatttc ccacctgata
     6121 accaccagga gcgatatccc acggttcagc aattaacttc acctgcgaga gcaccgggca
     6181 gttctggata gcggtaaaca acggcgcatc ctgacgaaac tctggcgtac ggcccatgat
     6241 tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt tctacccaat aacgcaggca
     6301 ggcgctggca taatctacca ccgccggatg actcaaattg agcgtgttgc cacaaccggt
     6361 ccagttgtga taatcgccgt cttctcttat ccaataatag ctacggttgt cgatcccacg
     6421 cagcgagaat aacgggccgt cgaggtccag ttccgcacta tggttgagca cgatatcaag
     6481 aatgacttcg atacccgctt tatgcagtgc tttgattgca tcgcgaaact catccagcgc
     6541 cgtttctggc gagcaggcat acgccggatg cagcgcaaac atcgccaccg ggttgtaacc
     6601 ccagtagtta ctaagcccca tacgttgcag acgtggttca ctggcaaatt gtgccaccgg
     6661 cagcagttcc agcgcggtaa tgcccaactg tttcaaatag ttgatcatca ccggatgccc
     6721 cagggcttta taagtgccac ggatctcgac cggaatctcc gggtgcaggt acgttaatcc
     6781 tttgacatgg gcttcataaa tgatggtgct gccccacggc gtgcgcggcg gagcatcatc
     6841 ttcccagtca tagtgatcaa ccaccactac gcatttcggc gcaatggcgg cgttgtcgcg
     6901 atagtcaggt tcattatgac cggcgtgcag cagcgggtta tctttaaact ccccgtaaat
     6961 ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta aagcgatgcc cctcggcagg
     7021 ttgccagggg ccatgaacgc gataaccata acgcaaaccc gggcgcgcat ccggcagata
     7081 accgtgccaa atgtcgccac tgtgccctgg caagtcatag cgatgttcct ggccattggc
     7141 atcaaagaca cacagctcta cccgctcggc atgagcggag aaaagtgtga agttgacgcc
     7201 ctgaccgtcg taatgcgcgc cgaggggagt gggtttgcca atggcgagtt gtgtcattct
     7261 gcctcccgaa ccagccagat agtggccaga ggtggtagcg tcaggctcag tgaatgctga
     7321 cgaccgtggc tggcaatctc atcgctgtgt actgcgccgc cattgcctgc attactgccg
     7381 tgatagtgca tggaatcggt attgaggatt tcacgccatt tacccggctg gttaataccg
     7441 aagcgataat catgacgcgg caccggtgta aagttactgg caacgatgat ttcgttaccc
     7501 tctttatcgc gacgcacaaa gatcagcacc gagcgttctt tgtcatccac caccagccat
     7561 tcaaagccgt acgggtcaaa atccagttca tgcattgctt tatggtggca gtaggtgtgg
     7621 ttcagatcgc gcaccagacg ctggacaccg tggtgccagt tatcgccgcc ttccaacaga
     7681 tgccagtcga ggctggcgtc atggttccac tcgcggccct gggcaaattc gttccccatg
     7741 aacagtagtt tcttgcccgg gaatgcccac atccagccgt agtaggcgcg caggttagcg
     7801 aatttctgcc atgcgtcgcc cggcatccgg tcgagaatcg attttttacc gtggaccact
     7861 tcatcatgcg acaacggcag gacgaagttt tcagtgtagt tgtagagcat cccgaaggtc
     7921 agtttatcgt gatgatactg acgataaatc gggtcgagct tcatgtagtc cagggtgtca
     7981 tgcatccagc cgaggttcca cttgtaccag aagcccagac cgcccatatc ctgcggacga
     8041 gaaacgccag ggaaatcggt agactcctcc gccattgtca ctgcaccgga aacctgctca
     8101 ccaagaatac ggttggtatt acgcaagaat tcaatcgctt caagattctc gcgaccgcca
     8161 aattcgttcg ggatccactc cccctcttta cggctgtagt cgcgataaat cattgacgcc
     8221 accgcatcga cgcgcagcgc atcaatacca aaacgttcaa tccagtaaag tgcgttaccg
     8281 acaaggaagt tactgacttc acggcgacca tagttgtaga tcagcgtgtt ccagtcctga
     8341 tgatagcctt cgcgcggatc gctgtgttca tacaagttcg tgccatcaaa ttcggcaagc
     8401 gcaaagtcat cggtcgggaa gtggcctggc acccagtcga gaatcacgtt cagaccagct
     8461 gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag taccaaaacg gcgggtcggt
     8521 gcatacaggc cggttggctg ataaccccaa ctgccatcga agggatgctc gttaatgggc
     8581 agtagctcga gatgggtaaa gcccatccat ttagcataag gcaccagttg atcggccagc
     8641 tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac gccaggagcc caggtgaact
     8701 tcataaatag agattggcgc atcaaactga ttcgcttttt tgcgctcttc agtctgcaca
     8761 accttttccg gcagcccgca aataagagac gcggtttccg ggcgcatttg cgcttcgaag
     8821 gcataagggt cggacttcag acgcaagttg ccattggcat caatcatctc gtatttatag
     8881 agctgaccgt tatgcgcccc ggggataaac agttcccaga ttccgctctc tttacgcagt
     8941 cgcatcggat gacggcgacc gtcccagtag ttgaattgcc caaccaccga gacccgacgg
     9001 gcgtttggag cccagacaga aaaacgcgta cctgtgacgc catccatagt atctgcatgc
     9061 gcgcctaagg tttcatacgg acgtaggtga gtaccttcag ataatagcca ggcatccatt
     9121 tcctggatta gcgggccaaa acggtaagga tcatcaatca ggttttgctg accatgccag
     9181 acaacagcca actgatagcg gaaaaaattc ttacgtcgcg gaatgacacc gctaaagaat
     9241 ccccgtgagt cgagacactc cagttttgcg agtttgcgcc cggttttcgg ttcaatcgcc
     9301 cacacatcgg tagcgtcggg taaaagggca cggacttcca gtcccgcggt ggttttatgc
     9361 atccccagta cggaaaaagg atccgcaaaa tggcctgcaa ttagcgcgtt aatcacgtct
     9421 ctatcgatac gatcggacat gcttgtcttc ctgttttatt gtgtcacccc atccaactga
     9481 atttttgact tctggttgtg acattttttt gacctgaacg gcgcagcact ctgtgcatcc
     9541 tctctgcgtc gtcctcactt caggtaaggc tgtgaatact cgtgtattcc gccaccctta
     9601 aagaatagcc aatgctctat ttaactcccg gtaaatcatg aaacatctgc gcttactcct
     9661 gtattacgca ctagcagggg cggcatcgcg ccccagattt aatgaataaa gattacgcca
     9721 gttgacgaag catccgacgc agcggctccg cggcccccca cagcagctgg tcgcccacgg
     9781 taaaggctga caggaactcc ggtcccatat tcagcttacg cagacggcct accggcgtgg
     9841 tcagcgtgcc ggtaacggca gctggggtta gctcacgcat agtgatttcc cgatcgtttg
     9901 gaacaacttt cgcccacgga ttgtgcgcag ccagcagttc ttccacggtc ggaatagaca
     9961 catctttttt caatttaata gtgaatgcct ggctgtggca gcgcaatgcc ccgacacgca
    10021 cacataaacc atctaccgga attacggaag atgtgttgag gatcttgttg gtttccgcct
    10081 gccctttcca ctcttcgcgg ctctgaccgt tatcaagctg tttgtcgatc cacggaatca
    10141 ggctacccgc cagcggcacg ccaaagttat ctaccggcag ctcaccgcta cgggttaagg
    10201 ttgtgacttt gcgttcgata tcgagaatag cagaggacgg gttcgcgagt tcatcagcca
    10261 catggccata cagatggccc atttgggtta ataactcacg catatgtcgc gcaccaccgc
    10321 cggaagcggc ctggtaggtt gcaacggaca cccaatcaac aagatcattg gcgaataaac
    10381 cacccagcga catcaacatc aggcttacgg tacagttacc gccaacaaaa gtcctgatac
    10441 cattatttaa tccgtcggta atgacgtcct gattgacggg gtcaagaatg atgatggcgt
    10501 catctttcat gcgcagagat gatgctgcgt caatccagta accttgccat ccgctttcac
    10561 gaagctttgg atagatttcg ttggtataat cgccgccctg acaggtcaca atgatatcga
    10621 gggcctttag cgcctccaga tcaaaggcat cctgaagtgt gccagtggtt ccgccaaaag
    10681 acggcgcagc ctgaccaagc tgagaagtag aaaagaagac agggcgaatg gcgtcgaagt
    10741 cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac cataccgcgc cagccgataa
    10801 aaccaacatt tttcataagc gttttttcct gcaaagatgt gtgctgtata aatgtgccgg
    10861 tctcctcctg gcacatcttt caccatacaa aaagcagcca aagtcgcaag tgaaattaat
    10921 caatgatagc gaagccatca gtaatgcgac ttatcctgct ttgttagcac gcagaaagtc
    10981 cgcggcaatt atcagggaat ttgagttatg aatgaaatca tttctgcaac agttttattg
    11041 atcctgatta tggatccact cggaaaccta cctattttca tgtccgtact gaaacatact
    11101 gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc ttattgctct ccttgtgatg
    11161 ctggtgttcc tgtttgcggg cgagaaaatt ctggcatttc ttagcctgcg ggcagaaacc
    11221 gtctccattt ctggcggcat cattctgttt ctgatcgcca ttaagatgat tttccccagc
    11281 gcttcaggaa atagcagcgg gcttccggca ggtgaagagc catttatcgt gccgttggca
    11341 attccgctgg tcgccgggcc gactattctc gccacgctga tgttgttgtc tcatcagtac
    11401 ccgaatcaga tgggacatct ggtgattgct ctgctgctgg cctggggcgg cacctttgtc
    11461 atcctgctac agtcttcgct atttttacgt ctgctgggcg agaaaggggt gaacgcactt
    11521 gaacgcctga tgggattgat tctggtgatg atggcaactc agatgttcct cgacggcatt
    11581 cgaatgtgga tgaaggggta acgaggagag tttttcgaaa agagcccggt agcgtcacaa
    11641 ctaccgggcg aacaaactta actcaacagc tggaacgcaa tcatcccaac gatggcaccg
    11701 acagtgccga ggatggtttc catcatggtc caggttttaa gcgtttcggc ttcggtcgcg
    11761 ccggtaaatt taccgaacaa ccagaaaccg gcgtcgttaa cgtggctgac aacaatcgaa
    11821 ccaccggcga tacaaatcga cagcgccgcc atttgcgcac cggagtagtt cagttgttca
    11881 atgaccggca ttaccaatcc caccgccgtt aaacaggcaa cggtagcaga accctgaatg
    11941 atgcgcactg cagctgccag cacgaagcag gtgatggcaa tcggcaggcc catgccggtt
    12001 aacgcttcgc ccagtgccgg accgacacca gagtcaacca gcacctgttt gaacacgccg
    12061 cccgcaccaa tcaccagcag aatgatcccc gccggttgca gcgcgtgacc gcaaatctcc
    12121 atcactttgt cttttggcat accctgacgc attgccagac cgtaaatcgc caccaggcaa
    12181 gcaaccagaa tcgcggtaaa cggatgacca ataaactcga accattcgta agcagtagag
    12241 ccttctggca caaaacgcgc ggcaatggtt ttcagcccca ccagcaccag tggcaacagg
    12301 atcagcgaca ggctgaatcc gaaggacggc attttgcctt cgccgagatg cggttcgctg
    12361 atgtcgtcag gaatatgcaa ctccacgtag cggctgatga aattacccca cagcggcccg
    12421 gcaataatca ttcccggaat tgccgcgcac aggccaatca ggatcatcca gccaaagtcg
    12481 gcgttcatct gcgatgccag cagcattggc gctggcccag gcaccaggaa cgcagccgct
    12541 gccgccacgc cggcgaataa tgggattacc agcttcacca ggttcgtgcc ggtgtggcgc
    12601 gccattgaga aagcaacgct aatcagcaga acaatcgcca cttcaaagaa cagcggtagc
    12661 gcacaaacca gccccgcaag gccgatagca taatgcacgc ggctgtgacc gaaggatttg
    12721 agcattttga cggcaatctg atcgaccgcg ccggtttcat gtaagatctt accaaacatg
    12781 gctcccaggg cgacaaccac cgccaggaag ccgagggtgc ctcccatccc tttttccatc
    12841 gtcgctgcga ttttatcgag cggcatacca gaaaaaaggc cagcccccat ggacaccacc
    12901 attaaagcca ggaaagcgtg catacgcgcc ttcatgacta aaaacagcag cagtaaaaca
    12961 gaccctactg ctgttaaaac aagcgttaat gtagtcacta cttatttgcc ttttttaata
    13021 acctcaatgg tgcttgccac aacaccttcc agcggttgat caatatccac caccagtaca
    13081 tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact gcgtcaccag catttgggtt
    13141 ttaaagaaat ggcctttgcg cgctttcagg cggctttcaa tcacatcaaa atcgcctttc
    13201 aggtagatga aagagagatt aggattacct tcacgcagca agtcgcgata gtgttttttc
    13261 agtgcagaac agacgatcag cgatacttta ttggtgcgct gcatagcaaa cgcggcgtcg
    13321 ttcagcgcct gcaaccacgg tttgcgatcg tcgtcattca gcggttcgcc agacgccatt
    13381 ttttcgatat tgcagcgcgg atggaggaaa tcgccatcaa gaaacgcggc atgaagttga
    13441 tgcgccactt cactggcgac cgcagatttg ccgctgcccg atacgcccat caagacgtaa
    13501 atgtggtgat catggttagt cgtgctcaaa gtggtgcccc cacaatacaa gaattaaaaa
    13561 tgttacgggt aactgttatc ggtaacattg tccagccgga caatatcaga agcaatatcc
    13621 atccgtgcct taagtgtata agtgtgagct acttcaaatt tgtaggctta aatagatccg
    13681 cccggtgaca aggtgaaacc taaatctaac attttcggtg tcacagattc gccacgaata
    13741 cgcgccagca ggcgctcagc gccaatgctg cccatccgct cacgcggcgt cagcacactc
    13801 gccagccgtg gctccatcac ctgaccaatg tcatgaccgt ggaaaccggc aatcgccata
    13861 tcgtcaggaa ctttcaaccc cagacgctga cattcaaacg ccgcgccgac cgccaggtca
    13921 tcattagtac agaacacgcc atccagctgc ggatattccc gccgcgcctg gcgaatcagt
    13981 tcaataccgg aagagtaaga agaagattgc tcaaccatca cgctatacgg caccaggcct
    14041 gcatccagca tcgcctgttc gtatcccttc tgtttgatga tagtacgttc gtcgaggcgt
    14101 gcgccgagat aggcaatgtg gcgatgcccg cgagcaataa tggcggtggt catctggcgt
    14161 gccgcttcaa agttatcaaa accgacggcg atatcgaggc acggcgactt actgtccatc
    14221 aactccacca ccggaatccc cgccacttca atcatcttta aggtacgcgg cgtgtgggta
    14281 cgttcggtga ggatcaggcc gtcgatattc catgagagca ttgattcgag gcgttcttgc
    14341 tccatttccg gtttataacc gtagtgcgcc agcatggttt gataaccgtg cgcgtcggtg
    14401 acgctttcga ttccgcgtaa tacttccgcg aaaacctggt tggtgagaga aggtaacagg
    14461 atgccaatcg cccggctggt ggcgttagag agtatatcgg gcgcgcgatt gggaatataa
    14521 cccagttcat caagagcggc ggcaatcttg ccgcgtagag cgacggaaac ctgctccggg
    14581 ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta cacggtcagc cacatcctga
    14641 agtacgggtc ttttcttttt catcgtcctg aaggtacaaa agagatagat tctcttagtt
    14701 taacacggac gcatcacaac cttcccgatt cctcaggaag gtttgtgaga taaatttaga
    14761 ctggcggtaa atcgaacagc aggatttcac tgtcgctatc agcatggatg gaaatcgcct
    14821 gctcatccca gattgccaga ccatcgctgg tcgaggcttt cacgccgtta atggtgacat
    14881 tgcctttcac cacctggatc cagacgcggc gttcagcggc aatctgatgc accgactgct
    14941 catctttcac cagcgcccaa cgatacagtt ccatatcctg atgcactttc aatgaaccat
    15001 cacgcgcatc cggcgaaagc accagttgtt tgccctgcac ggcatcgaag cgacgctgtt
    15061 cataacgcgg cgtaatgccg ttttcttcag gcatgatcca gatctgatac agatgcagac
    15121 gctcggtgct gcttgggttg tactctgagt gacgaatacc cgtacccgca ctcataatct
    15181 ggaactcacc cgccggaacc tgctctttat tgcccatgct gtcctgatgc tcaacagtac
    15241 cttccagcac gtaggtcaaa atttccatat ctttatgcgg gtgagtgccg aagccctgcc
    15301 ctgcttcaat cacgtcgtcg ttaatcacgc gcagcgcgga gaagcccata aagttcggat
    15361 cgtaatagtt ggcaaaagag aaagtatgcc aggagtccag ccagccatga tttgcatgac
    15421 cacgttcatt tgctttgcgt aagtagatca tttgtattcg ccccctgaat gatttcgatg
    15481 ggcttagtgt ggacccgatc cgcctgggat gatagagggt gaaaattgac ccctctgttc
    15541 aaaaaaattg aactattcga ggggccatca aacttacttc gcgagagtca ctgtggaggg
    15601 agaagcttgc tcaaaaccgc gttcaaggat ttccagattg gtaagaactt cagattcctt
    15661 gacgtaattt ggcgcaccgt tggtgatggt ttgatacaac gcatcataaa cgcgcccata
    15721 atcgcccatc tctggcttca tctcttctct taccgtcacg ccctcgtcat tgacatactc
    15781 cagcacaccg accgaatcat ccgctgcgaa tcctggttcg cccggcataa tattagcctt
    15841 caggctggtt tcctgctggt cgataccgta tttaataaac gaacctttct taccgtgaac
    15901 gataaatttc ggataatcga ttttcaccag atggctggtt ttgacgatgg cttttagatc
    15961 gccataaaac agctgcgctt caaaggtgtc gtccggattg gctttattac gcaggctgcg
    16021 gatgtcataa gcgacgtgat ccgggcgacc gaacagagaa ataatctggt ccatcgtatg
    16081 cacaccaagg ccatagaacg cgccatcctg cggcagcccc ggtttggttt ctgccaccgg
    16141 gcggtaatag tcaaaatggc tttccacttc gacaatctct cccagcttgc cgctttcaat
    16201 cgcttttttc gctgtcagga agcaggaatc aaagcgacga ttctgatacg gcgtaacgat
    16261 cagccctttg cttttcgcca gtgcaaacag ctctttcgcc tgcgcaattg tcggagtgaa
    16321 cggtttttcg accagcacat ttttcccggc ttccagcgcg cgcttcgcgt actcgaagtg
    16381 gctgtccgcg tgggtgcaga cgacaaccag cttaacatcg ggatcgttta gcacttcgtc
    16441 gagatcgctg gtgaaatgga tatgggaata aatgggggcc tgttcttccg gcttcgcatg
    16501 gcggcgaaaa atatgcgcga catgccagct atccttgcgg ttaagtacat acggcagatg
    16561 gtaacgggtg gtgcttttgc cgaagccaat aaaggcgcag ttaatgacca tgatttcgtc
    16621 ctttttaagg tggttattta caccttagcg caaagcggac gtggttccta ctggagtgcg
    16681 cataacgaac acaagcactt cccgaggata aattgagaac gaaaggtcaa aaaaaaagcc
    16741 agcacccggc tggctaagta atactggaag caatgtgagc aatgtcgtgc tttcaggttc
    16801 tccgcgaggg tcttcctgat cgcgagacaa taataatcat tctcattcgc acttgtccaa
    16861 cactttttgc aaaaaaatgc atttgactcg catttgaaag tcaatgatgt tgaaagggac
    16921 atttacccca aagaggacaa aggaatgagt gagatagtaa tacgccacgc agaaacacgc
    16981 gatcacgagg ccatcaggca gattcacgcc cagccggagg tgtattacaa cacgctacag
    17041 gtgcctcatc cttcagaaca aatgtggctg gaacgactca ccgctcgccc tggcatcaag
    17101 cagttagtcg cctgtattga tggaatcgtc gtaggccatc tcaccattga cgtgcaacaa
    17161 cgcccacgcc gcagtcatgt tgccgatttt ggtatctgtg tcgactcccg ttggaagaac
    17221 cgcggcgtcg ccagcgccct gatgcgagag atgattgaaa tgtgcgacaa ctggttgcgg
    17281 gtagatcgta ttgagctaac ggtgttcgtc gataacgcgc cagcaattaa ggtctataaa
    17341 aaatttggct ttgagattga agggactggc aagaagtacg cattacgtaa tggtgaatat
    17401 gtcgatgcgt attatatggc gcgggtgaaa tagagaattt gcaatctacg tttgtcattt
    17461 aagccctcac ctaccgggag agggcttaag atattaatac cccgccgtta aatcatccac
    17521 cgagcgcggg tcggatgcac cgtacagctc accgtccggc ccaatcataa tgctttgtgt
    17581 actgcccatc gcctctttca gcgccacttt ctgacctttt gcttccagca gcttgatcgt
    17641 atccgggcta aacccttttt cgacacgcag ctcgtccggc aaccactgat ggtggaaacg
    17701 cggcgcattg gtcgcttcgg cgacgttcat tccataatcg atgctattca ccaccatttg
    17761 cagcacagtg gtgataatcc ggctaccgcc tgggcttccg gtgaccagcc aggttttacc
    17821 atccttgacc acaatggttg gtgacatcga cgacagcggg cgtttattcg gcccgacggc
    17881 gttggcatca ccgcccacca gcccgtaaac gttcggtacg cctggtttgg cggagaaatc
    17941 atccatttgg ttattaagca gaataccgct ctcgcccgcg acaatgcccg taccgaaggt
    18001 ggtattcagc gtataggtca ccgccacggc gttaccgtct ttatccacta ctgagtaatg
    18061 ggtagtttga ttactctcat aaggcgcaag cttgccagga cgaatttcgc tggatggctt
    18121 cgctttattg atatcgattt gatcggcaat agatttggca taggctttat tggtcagcgc
    18181 ctgccacggt actttgacaa aatccgggtc gccaagatat tccgagcggt cggcataggc
    18241 gtatttctca gcttctgcca tgatttgcat cgcgtcggca ctgccaaagc cgtacttctg
    18301 catatcgaag ttttccagaa tattgaggat ttgtacgata tgaataccgc cggaagatgg
    18361 cggtggcatg gagtaaacct gatacccgcg ataatcgccg cttatcggtg tgcgttcgac
    18421 cgctttatat gccgctaaat cttctttgct gatcaagcca ccgtttttct gcatctcctg
    18481 ggcaatctgt tccgctatcg tgcctttgta gaattcatcc gggccgtttt cggcaatcat
    18541 ctccaggctc tttgccaggt tcgcctgcac cagcttgtcg ccctttttca gcggttcgcc
    18601 ctctttccag aagatagctt tgctgttttc gtggttcggc agcacttcac tgccgtaggt
    18661 tttgagatcg tcagccagcg cgtcgttaac gataaaacca tcgcgtgcca gtttaaacgc
    18721 cggttgcacc actttattca acggcatggt gccgtattta tccagcgcca acgagaaacc
    18781 cgctaccgta ccgggtgtgc cggaagccag atgcgaagtg agtgattttt tgctgtccgg
    18841 gttgccctga tcatcgagga acatatcgcg ggtcgctttg gcgggtgcca tttcgcggaa
    18901 atcgatagcc gtggtattgc catttttcga gcggatcagc ataaaaccac cgccgcccag
    18961 attccctgcc tgcggatgcg ttactgccag cgcgtagccc accgccacgg cggcatcaac
    19021 ggcattcccg ccctccttga ggatatccac ccccacctga gtggcagtag catctacaga
    19081 cgccaccatt ccctgcttag cgcgtaccgg atgaaagaca tcttcctcca caccataaga
    19141 aacaggtggc gcggctaccg tactaaaaca acttcctgtg agcagagcag caatggccac
    19201 ccggcgtaaa aacgtcggtt ttatcatcgt tattctccag agattaaggg gcaaccccag
    19261 ctaagcctgg tatataactc tgaattaatc atcgttttgc cgggaagcga gtaaacttaa
    19321 agaatatctt cagaggagga tacgcgatga aacgacttct gcttttgacg gcactcctgc
    19381 cgtttgtcgg ctttgcacag cccattaata ctctgaacaa ccctaaccag ccgggatatc
    19441 agatccccag ccagcagcgg atgcaaactc aaatgcagac gcagcaaatc cagcaaaaag
    19501 ggatgctgaa ccagcaactg aaaacgcaaa cgcagctgca acagcagcat ttagaaaacc
    19561 agataaacaa taattctcag cgggtgttgc agtcacagcc gggggagcga aatcccgccc
    19621 ggcagcaaat gctgcccaac accaacggcg ggatgttaaa cagcaaccgt aatccggata
    19681 gttcgttgaa tcagcagcat atgctgccgg agaggagaaa cggcgacatg ctgaatcagc
    19741 ccagcacgcc gcagcctgac attccgttga aaactattgg gccgtaaagt tcggaccaat
    19801 cacgtcaatc gcatcggtac agatgcaatc cacgccccag cgcagcaact ctgccgcgcg
    19861 ctggggttta ttgacggtat aaaccagaat ccgtaatccg gcatctttca actgcatcac
    19921 tcgcgcttta tcgagcaact tatgattgag atgaatagag acgcagccca gtcgcgcggt
    19981 caattctcgc cagtcgtcgc gccattcatc aagcaataaa ccacgcggca gttccggtgc
    20041 tgcctgttgc gcggcttcta aagcatcaat ctcaaacgat gacagcagcg gcggcgtcat
    20101 accggtccac agttcgcgtg ccgccaacgc caccgttttg cccgttaatg gtccagtgcc
    20161 agtggtgggt ttgatttcga tattcgccat catcccgtgt tcgcggcagc gttccgccac
    20221 ctgcgaaagc aacggcagcg gctcaccttt aaacgctttg ctgtaccaac tgcctgcatc
    20281 cacgcgcagt aaatcctgcc agttcagctc gcccgcaacg ccccaaccgt tgctggtacg
    20341 ttcgagattg tcgtcatgga gcaggaagat ctcgccatct ttcgataact ttgcgtcaaa
    20401 ttcgatcatc ttatgaccgt atcttgcccc gacgtcgatt gccgccagag tgttttccgg
    20461 ggccagctta ccgccaccac gatgggcgac gatgcgggga taaggccagt tactcatact
    20521 cgttgtcctg tttcaccatc aaaaagatgc agctgatttt ccggcagatg cagccacagc
    20581 gtgctgcctg ccgtcgggcg ctcctgatgc gccagtcgca ccaccagctt ctgttcgccc
    20641 cagcgtccgt gcgccaggtt atctgcgccg aggatctcca gcgtgtccat caccagcggc
    20701 acaccacctt ctgcccgcga gcttagcgct atatgttccg ggcgaatgcc gagggtcatt
    20761 ttacgcccgg catactgacg gtagccaccg tttagcagca gcgtaatacc accatccagt
    20821 tcgaaatgcg taccttcgtt attcacgcgg ccagccagta ggttcatcgc cgggctgccg
    20881 ataaaactcg ccacaaacag gctggcgggc ttttcgtaca cttcaactgg cgtgccaatc
    20941 tgttcggcga cgccgccgtt catcaccatt actcgctggg cgagcgtcat cgcctcaacc
    21001 tgatcgtggg taacgtagag tgaagtcgtt ttcaggcgac ggtgcagctg ttgcagttca
    21061 agacgcatct gcacacgcaa cttggcatcg aggttagaga gcggctcatc aaacaggaac
    21121 actgccggct cacgcacaat cgcacgcccc atcgccacac gctgacgctg accgccggat
    21181 agctcacgcg ggcggcgctt aagcaggccg tccagctcca gaatgcgcgc cgcttcttta
    21241 acacgttcgg caatttgttg cttgcccatg ctgcgaattt tcagccccca cgccatgttc
    21301 tcctctacgc tcatatgcgg atagagcgcg tagttctgga acaccatcgc aatcccgcga
    21361 tctttcggct ccatttcggt gacacgcttg cggtcgatcc agatatcgcc cgtcgttacc
    21421 cgctccagtc cggccaccat tcgcaataac gtcgatttcc cgcagccaga cggcccgacc
    21481 atcacgataa attcgccatc cgccacatca agggtcagcg gtttaatgac ctgggttttg
    21541 ccatcccagc ttttggttac tgcctgtaat ttcagtcctg ccatcttatt tctcactatc
    21601 gaccaggccg cgcacgaagg cacgctgcat cactaaaaca atcactactg gtgggatcag
    21661 ggttaacaac atcgccgcca tcaccgagtt ccattccgtt gtgccttcgc ctgtggcgat
    21721 catcccttta atccctgcca cggtggtgcc gaggtcaacg tcggtaataa tcaacaacgg
    21781 ccacaaatac tggttccagc cgtaaataaa ggtgatcaca aacagcgccg ccagattagt
    21841 tttggagagc ggaaaaacga tgtcgcaaaa gaagcgcatt ggtgacgcac cgtcgatccg
    21901 cgccgcttcc atcagctcat ccggcagcgt cataaagaac tggcggaaca ggaaggtagc
    21961 ggtcgccgag gccatcagcg gcagcgttaa accggcgtaa ctgtcgagca tcttcaggtt
    22021 ggcgatgact tccaccgtcg ggaaaatacg cacttcaacc ggcaacatca gagtgataaa
    22081 aatcatccag aagaagaggt tacgtagtgg aaaacgaaac cagacaatgg caaatgccga
    22141 gagcatcgag acggtaattt tgccgagcgt aatgctaaac gccatcacaa agctgttaag
    22201 caacatccgc cagaacggcg cactattcgt gcctaccccg ttcacccaga tgttgtggat
    22261 gttttccagc agatgcgtgc cgggaataag cgtcatcggc gcggcgtaga cttcttgttt
    22321 atccagcgtc gccgcgacaa acgccacgta cagcgggaag aggatcaccg cgatcccgag
    22381 gatcagcatg gtatggctga atattgtcag ccacggacgg ttctcaatca ttggtaacgc
    22441 accttgcttt caacatagcg gaactgcacc accgtcagca cgatgacgag gaacatcaac
    22501 accaccgact gcgcggcaga ggaagccaaa tccagtccgg taaagccttc gcggtagatc
    22561 ttataaatca gcgtcgtggt ggcctgtact ggcccgccgg acgtggcggc gtcgatcacc
    22621 gggaaggtgt cgaagaatgc atacaccagg ttcaccacca acaggaagaa actcaccggg
    22681 gcgataagcg gcaacgcaat cttaaagaag cggcgaattg gacctgcgcc gtcgatggct
    22741 gcggcttcaa tcaacgaacg gggaatggat tgcagcgcgg catagaagaa caggaagttg
    22801 tagctgattt gcttccacac cgaggcaaac accaccagga acatcgcctg accgctgttt
    22861 tgcgcgtggt tccagtcgta gccgaactca gcgagaaaat gggtgatcag cccgcgacca
    22921 gggttaaaca gaaagatcca caatacggcg gcaacggcgg gagccacggc atacggcagc
    22981 agcatcaagg tttgatagaa acgactgccg cgcacgatgt actccaccag cgctgcgaag
    23041 aacagcgata ccagcaaacc gctgacggtg acaaaggtgc tgaattttat cgtcgtccag
    23101 aaggcgtcga gatagtagct gtcgtgaaac agcgtgacga aattatccag gccgacaaac
    23161 tggctggaga aaccaaacgg atcgacgctt tgtagcgagt accacaacgc ttcgcccgca
    23221 ggccagataa aaaagataac ggtgatgatg agctgcggcg cgaccagcaa ataaggcagc
    23281 cagcgcgagc ggaacaccgg acgggatgat gacattacag ttaattcctg aactgtgccg
    23341 gatgcgcttc gcttatctgg cctacacgcg atgccaggtc agataagcga aacgcatccg
    23401 acattacact gattaagact tcgtcgattt ctcaaagcgg cgcagtaact ggttcccacg
    23461 ctcaacggca gtatccagcg cctgctgtgg tgtcttctta ccggtccaca cgctctccag
    23521 ctcttcatcc acaatcacgc ggatctgcgg catgttgccc agacgcaaac ctttggtgaa
    23581 cggcaacggt ggcttgttca gcatctgacg cgtggcaata tccgctcctg ggtttttctc
    23641 gtaaaagccc tgctcacgcg tcaggtcata cgccgctttg gtgattggca gataaccggt
    23701 tttctgatgc cactcggcag cgttttctgg cttcgcgagg aagtcgagga acttcgccac
    23761 accggtatac gtttctttat ctttgccctg catcacccac aggctggccc cgccgataat
    23821 ggcgttttgt ggcgcatctt tcgcgtcagc gtcgtaaggc atcatgccta cgccgtagtt
    23881 gaatttggcg tactcgcgaa tgttggcaag agaaccggaa gaggcagtcg tcatcgcgca
    23941 atcaccgtta tagaacttct cggtggattc atccttacgc ccgacgtagc tgaaatcgcc
    24001 cttcttgttc atctcttcga gcatggcgat gtgtttcacc tgctccggct tgttgaactc
    24061 cagcaccgcg tctgtgccgt caaagccgtt gtttttgctg gcaaacggca gaccgttcca
    24121 ggcgctaaag ttttccagtt ggatccatcc ctgccagccg ctggcgtagc cgcacttcat
    24181 gccggaggct tttagttttg cggcatagtc cgccagatcc tgccaggttt tcggcggttg
    24241 ttccgggtct aatcctgctt tcttgaaggc gtctttgttg taatagagaa cgggggtcga
    24301 actgttgaac ggctgggaaa gtaagtgccc agttttgctg tcggagtagt aaccggaaac
    24361 cgtcggcaca aactgcgact catcgaattg aatccccgcc tctttaaaca cgtcatacac
    24421 cggtttaatg gctttcgacg ccatcatggt ggcggtgcca acttcataaa cctgcaaaat
    24481 agccggagcg ttgccggtac gaaatgcggc aatccccgcg cttaaattct gttcgtagtt
    24541 gcctttatag gtcggtacaa ttttgtaatc cgggttttcg gcgttaaaac gttgggccag
    24601 agaatccacc tctttaccca gttccccttc catagaatgc cagaacggaa tggtcgtcac
    24661 tgcctgtgca ttccccatta aagccagtcc gagcgccagt gctgaagctg tataacgtaa
    24721 cggtttcatt gattatctct cttgttgtac cgaatgcgcg aattcacgcg ttttatgctc
    24781 gcggagtaac atgacatgct cgaattacag aaaaataact attttgttac atttttaaga
    24841 tagtaaggtg tcagaaagat gacaaggcgg tgacggcgcg ggcgagggaa aatgggagat
    24901 ggggcacgga taaaaaacag aatggttaag aaccttgtca ggttccgtgt tgacgcaagc
    24961 gggtaacgat atcttctaac gatacttttc ccgccgccac atcaaccgtc aattccacaa
    25021 actctgggtt tgcagcaagt atgaggccgt tacgtttaag gaataatagc gtgataaaca
    25081 gtgcggtgcg cttattacca tcattaaaaa tatgaccgcg cgagattgcc aatagatgca
    25141 tcgccgccag cagccaaaga tcggaaacgc cttcatactc gtacttattc agtacccggt
    25201 acaaaagcgc ttctgctcgc cctggatcgg acattcctgt tacacctggt gtaacgctaa
    25261 gcaacttatc gtggaaataa attatctcct ccgcagagat aaactgaatg gtcatttatc
    25321 cgctaactcc ataatttcgc tgccatgaat agccatgatg gcgtcaaatt ccgcgtccag
    25381 tttagccttc tggtaacgtt caaactcttc tgcgctaata atcacagcgg ccttatgccc
    25441 acgcctcgtg atcgttaccg gagtacctgt cgccgctgtt tccagaacca atgcaaggtt
    25501 ctgtcgcgct tcgctgtagt tcatggtatg catgatatcc tccatattga gtacaattaa
    25561 attgtacaac taacaagtac aaaaaaggag tcattcgact cctttttact caaaatgtta
    25621 ttatccgcct aaatatgcac ttctcactgc ttcattcgcc agcagcgcgt cgccggtatc
    25681 ggagagcact acatgaccgt tttccagcac gtagccgcga tccgccagct ttagcgcctg
    25741 gttggcgttc tgctcgacaa ggaagatggt catcccctgc tcgcgcagtt gttcgatggt
    25801 gtcgaaaatt tgctggatga taatcggtgc cagcccgagc gatggctcat caagcaacaa
    25861 caaacgcggg ttgctcatca gcgcgcgacc aatcgccagc atctgctgtt caccgccaga
    25921 catagtgccc gctcgctgaa cacggcgctc atgcagacgt ggaaacagtt catagaccca
    25981 ctttatgcgc tcctggaact ggtcgcgttc ggcaaaaaag ccgcccatcg ccaggttctc
    26041 ttccaccgtc atccgcgaga agacacgacg cccttccggg acaatcgcta ccgcttcgcg
    26101 catgattttc gccgtctgcc agtcggtaat gtctttatca tcaaacacaa ttcgcccgct
    26161 ggtggcgcgc ggatcgccgc ataacgtgcc gagcaaggtg gttttccccg ccccgttcgc
    26221 gccaatgagg gtgacaatct cgccctgatt gatatgcagg ctcacctcat gcagcgcctg
    26281 gattttgccg tagtgggcgc tgactttgtc aaaggacaac atgacttttt ccatcttatg
    26341 cctcacctaa ataggcacgg atcacatccg ggttattacg gatctgctcc ggtgtaccgt
    26401 ttgccagcgg cgtcccctga ttgaccacgt aaattctgtc tgaaatcccc atcaccagct
    26461 tcatatcatg ttcaatcaac aagatagtgg tgttgtgatg gttacgcagt tcggcaatca
    26521 gctcatccag ctctttcgtc tctttcgggt taagacctgc tgccggttcg tcgagcatta
    26581 aaatctccgg ctgcgtcacc atacagcggg caatctccag acgacgctgg tcaccatagg
    26641 ccaggttact cgcctgacgg ttggcatgtt ccaacaaacc aatgcgctca agccaggtag
    26701 cggcgcggtc gagcgcttcg ctctgggcgc gacggaaaga gggggttttc aacaggccgg
    26761 agaacagccc agttttcagt tgctgatgct gtgccaccag caggttttca atcaccgtca
    26821 tttcacggaa cagacgtaca tgctggaagg tgcgcaccac gcccatgcgg gcaatttgct
    26881 gccccggtaa accttccagg tgctgatcgc gcagtaaaat ggtgccgccg gtgggtttat
    26941 agaatccggt caggcagttg aagaccgtgg tttttccggc accgttaggg ccgattaacg
    27001 agacgatttc ctgcgggtac agttcaagat tgacgttgtt caccgccagc aggccgccga
    27061 agcgcatcat cagaccgtta acagataata atgtctgact catgcctgct ctcctttcgc
    27121 tgcgccgttc ttcagcttga gttgcgggcg cgtcatgggc agcaagccct gcggacgcca
    27181 gatcatcatc agcaccatca aaccaccaag cattaacatg ctgtattcgt tgaaatcacg
    27241 catcaactcg cgcgatacca ccagcaaaac agccgccaga atcactgcaa attgcgagcc
    27301 cataccgccg agcaccacta tcgccagcac aaacgccgat tcggcaaaag tgaaggattc
    27361 cgggctgaca aagccctgac gcgcagcaaa cagcgttccg gcaaaaccag caaacgcggc
    27421 gctgatggta aaggcagtca gcttgatacg acgcgggctt aagcccagcg aacggcaggc
    27481 gatctcgtct tcacgcaacg cttcccacgc acggcccagc ggcatccgca gcaggcggtt
    27541 aatgacaaac aggcttaaca ccaccagcag caacgccacc aggtagagga agatgacgcg
    27601 atcggaggga tcgtatttca ggccaaagaa attactgaac gtgtcccagc cgccttcgcg
    27661 ggcggtacgg ctgaactcga gtccgaagag cgtcggtttc ggaatctgac tgataccgtt
    27721 cggtccgccg gtaatttcgg tgttattgag cagcaatatg cgcacaattt cgccgaaccc
    27781 gagggtaacg atcgccagat agtcaccgcg cagacgcagc accggaaaac cgagcaggaa
    27841 gcccgccgcc gccgccatca acccggcaat cggcagacag gtccagaagc ccagaccgta
    27901 atagtgattg agcagcgcaa aagtgtaagc gccgatggcg taaaagccgc cgtatcccag
    27961 caccagcaga ccagaaagac caacaaccac gttcagcccg agaccgagaa taatgtagat
    28021 catggtcaga gtggcgatat ccaccgtccc acgcgacacc ataaacggcc acgccactgc
    28081 aagcaccagc agcgccacga ggaacagttt ctgcttcacc gtggagccat caatcgcggg
    28141 cagaataaac ttcggcccgg aaacgctttt tagccctttc tggaaagcag gccgcacaag
    28201 ctggaagaaa aagaccaccg ccgtgccgat aaacacccac tgccaacgga tctctgaagc
    28261 cgtgtcgacc accagtttgg tgccatccag ctccagttgc acgcccataa agacgcccgc
    28321 cagcacaaag aacatagcgg cagagagcag cgccattgca atatgcatcg gtttcatact
    28381 ttctctacct ccgggcgacc cagaataccg gtcggcatca ccagcagcac caggatcagc
    28441 agggcgaatg agaccacatc tttatattcc gtactcagat aggcagaaga gagcgcctcc
    28501 gcaatcccca ggatcagccc gccaatcatc gcccccggaa tgctgccaat cccaccgagc
    28561 accgcagcgg taaaggcttt catcccggcc ataaagccga tgtaagggtt aattacgccg
    28621 tagaactgac cgagcagcac gcccgccact gccgccatcg ccgcgccaat cacaaaggtc
    28681 agcgcaatca cccggtcggt gttgatgcca agcaggctcg ccattttcag atcttccgcg
    28741 caggcacggc aggcgcgccc catacgggaa tagcgaatga aaatcgtcag cgccagcatg
    28801 gcgaggaaag taacaatcca tatcaccgcc tgcatggtgg taatagacgc agagaagttt
    28861 tcgctatggc ccaccaccca ctgaccgtta aacaggctgg gcagcgccac atcgcgcgaa
    28921 ccttcggtca ggctgacgta gttttgcagg aagatagaca tgccgattgc cgagatgagt
    28981 gcaatcaggc gcttagagtt acgcaccggg cgataggcta cccgttcgat actccagccg
    29041 taggcgctgg caatgacgat tgcgccgacg aatcccgccg ccaccagcaa ccagccggta
    29101 tcaatgccca gcatcatcag tgcggcgatg atcataaagg agacgtagct gccaatcatg
    29161 taaacctcgc cgtgggcgaa gttgatcatg ccgataatgc cgtaaaccat ggtgtagccg
    29221 atggctatca gcgcgtaggt actgcccagc gtgacgccgt taaacatctg ctgcaagaaa
    29281 tacaaaaact gctcagacat aaggtaacct ttctaaaccc gcccgttttt cacgaacggt
    29341 gggatgatca cttggctgcc gtggatgaac cgtcggcatg ccactggaag acaccaaaat
    29401 cgaatccctt aagatcgcct ttttcatccc agttcagcgg cccaattacg gtgtttgcac
    29461 cgttagcttt taaatctttc accagcgcca gcggctcatc gctgccggta cgttcaaggg
    29521 cagttgccag agattgcacc gccgcgtagg tgatccagac atagggcccg gacggatctt
    29581 tcttgtcggc tttcagcgca tcaacgatgc cctggtttgt cggatcctgg tcatagcgtt
    29641 ttggcatggt gaccaacatg ccttcagcgg catcaccggc aatgttcgac agcgacgcat
    29701 taccgacacc ttccggcccc ataaactggg ttttcaagcc aacggaacgg gcctggcgca
    29761 gcatctgccc catttccggg tagtaaccgc cgtagtaaac gaagtcgatg ttttcttttt
    29821 tcaggcgggc gatcagcgcg gagaaatctt tctctccagc ggtaatgccg tcgaagaaga
    29881 cgacgttggc gttagccgct ttcagcccgt cctgcaccga acgtgccagc ccttcgccat
    29941 actgttgttt gtcgtgaatg atggcgatgc gctggggctt caccgtctca agaatgtatt
    30001 ttgccgccgt tggcccctgg gaagagtcca gcccggcagt acgcatgatg tattgataac
    30061 cgcgttgggt cagctccgga ttggtcgctc ccggcgagat catcagaata ccctcgtctt
    30121 catagatatc tgatgcaggt tgggtagagg aggaacacag atgaccaata acatatttaa
    30181 tgccgtcgtt aacgattttg ttggcgaccg caacggcttg tttcgggtcg caggcgtcgt
    30241 catattccac gccaaccagt ttatcgccct taattccccc tttggcatta atgtctttaa
    30301 ttgcctgacg cgcgccgtta aattccatat cgccccactg ggcaatcggg ccggacatcg
    30361 cgccgacaac ggcgacttta atatcgtcag ccatagcggt gtgtgaaatt gtcagtgcaa
    30421 tcatccctgc gatgatagtt ttcgcattcc gtttcatagt caaaaatccc cattcgtgat
    30481 gttgtgttgc tttgttttta tgtgttaaca aatcagactg ttcttttttt atactgcact
    30541 gtttttacct gtctgattaa gggtttagcg cagtattttg tgatgatagc gattaaaatc
    30601 cctatttttc agtcgattaa gaacagataa tattctgaat ttattgatag ataaacagaa
    30661 aaaagcgccg ttgtcagcat aaaataacgg tacaaagggc agaataattc actaccattt
    30721 aggggattat gctggatatt tttcattctc taatgtttta actttgtaa
//
LOCUS       DAZFJL010000046        30718 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFJL010000046 DAZFJL010000000
VERSION     DAZFJL010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.046"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(160..1635)
                     /gene="ampG"
                     /locus_tag="ACHJ8C_002030"
     CDS             complement(160..1635)
                     /gene="ampG"
                     /locus_tag="ACHJ8C_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO5551889.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGISLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLM"
     gene            complement(1679..2257)
                     /gene="yajG"
                     /locus_tag="ACHJ8C_002031"
     CDS             complement(1679..2257)
                     /gene="yajG"
                     /locus_tag="ACHJ8C_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5551890.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2562..2879
                     /gene="bolA"
                     /locus_tag="ACHJ8C_002032"
     CDS             2562..2879
                     /gene="bolA"
                     /locus_tag="ACHJ8C_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO5551891.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            3223..4521
                     /gene="tig"
                     /locus_tag="ACHJ8C_002033"
     CDS             3223..4521
                     /gene="tig"
                     /locus_tag="ACHJ8C_002033"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO5551892.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            4767..5390
                     /gene="clpP"
                     /locus_tag="ACHJ8C_002034"
     CDS             4767..5390
                     /gene="clpP"
                     /locus_tag="ACHJ8C_002034"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO5551893.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            5516..6790
                     /gene="clpX"
                     /locus_tag="ACHJ8C_002035"
     CDS             5516..6790
                     /gene="clpX"
                     /locus_tag="ACHJ8C_002035"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO5551894.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            6978..9332
                     /gene="lon"
                     /locus_tag="ACHJ8C_002036"
     CDS             6978..9332
                     /gene="lon"
                     /locus_tag="ACHJ8C_002036"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO5551895.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            9523..9813
                     /gene="hupB"
                     /locus_tag="ACHJ8C_002037"
     CDS             9523..9813
                     /gene="hupB"
                     /locus_tag="ACHJ8C_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO5551896.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            10005..11876
                     /gene="ppiD"
                     /locus_tag="ACHJ8C_002038"
     CDS             10005..11876
                     /gene="ppiD"
                     /locus_tag="ACHJ8C_002038"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO5551897.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            12027..12398
                     /gene="ybaV"
                     /locus_tag="ACHJ8C_002039"
     CDS             12027..12398
                     /gene="ybaV"
                     /locus_tag="ACHJ8C_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO5551898.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            12504..12902
                     /gene="fadM"
                     /locus_tag="ACHJ8C_002040"
     CDS             12504..12902
                     /gene="fadM"
                     /locus_tag="ACHJ8C_002040"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO5551899.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWHGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(12954..13649)
                     /gene="queC"
                     /locus_tag="ACHJ8C_002041"
     CDS             complement(12954..13649)
                     /gene="queC"
                     /locus_tag="ACHJ8C_002041"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO5551900.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(13714..15414)
                     /gene="ybaE"
                     /locus_tag="ACHJ8C_002042"
     CDS             complement(13714..15414)
                     /gene="ybaE"
                     /locus_tag="ACHJ8C_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO5551901.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHLPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15514..16332
                     /gene="cof"
                     /locus_tag="ACHJ8C_002043"
     CDS             15514..16332
                     /gene="cof"
                     /locus_tag="ACHJ8C_002043"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO5551902.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKENPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLHEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            16485..16943
                     /gene="decR"
                     /locus_tag="ACHJ8C_002044"
     CDS             16485..16943
                     /gene="decR"
                     /locus_tag="ACHJ8C_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO5551903.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            16973..18745
                     /gene="mdlA"
                     /locus_tag="ACHJ8C_002045"
     CDS             16973..18745
                     /gene="mdlA"
                     /locus_tag="ACHJ8C_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO5551904.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRTMLAEAPVVIDGSDKVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMVGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            18738..20519
                     /gene="mdlB"
                     /locus_tag="ACHJ8C_002046"
     CDS             18738..20519
                     /gene="mdlB"
                     /locus_tag="ACHJ8C_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO5551905.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIDVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSTLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTLLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            20700..21038
                     /gene="glnK"
                     /locus_tag="ACHJ8C_002047"
     CDS             20700..21038
                     /gene="glnK"
                     /locus_tag="ACHJ8C_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO5551906.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            21068..22354
                     /gene="amtB"
                     /locus_tag="ACHJ8C_002048"
     CDS             21068..22354
                     /gene="amtB"
                     /locus_tag="ACHJ8C_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO5551907.1"
                     /translation="MKIATIKTGLTSLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTVPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNING
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(22403..23263)
                     /gene="tesB"
                     /locus_tag="ACHJ8C_002049"
     CDS             complement(22403..23263)
                     /gene="tesB"
                     /locus_tag="ACHJ8C_002049"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO5551908.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPDERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVSAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            23481..24053
                     /gene="ybaY"
                     /locus_tag="ACHJ8C_002050"
     CDS             23481..24053
                     /gene="ybaY"
                     /locus_tag="ACHJ8C_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO5551909.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            24243..25616
                     /locus_tag="ACHJ8C_002051"
     CDS             24243..25616
                     /locus_tag="ACHJ8C_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISEc13 family transposase"
                     /protein_id="HFO5551910.1"
                     /translation="MVLSDCYSWANEQFGHARLGDPRRTRRLVSLASSLAQHAGLSIV
                     KSSQSTAQVEGAYRLMRNPSISPEAIAEAGFTATARACEAHPLLLALEDTTTINFSHS
                     TAFDDLGNTTTNPKTRGLLAHSVLMYAPDSALPVGLIEQQRWSRATDTYGVKHQRKER
                     PYEEKESYRWQQASERMAERLGEIQKRVITVCDREADIWHYLHYKVSHGQRFVVRAAQ
                     NRRLEEAPGKLFELPEVLATAGSHTLNVMQKGGRAARQARMFISYSEVSIKNPDNSGQ
                     ALPLTYVCCREQAEDGACWHLLTSEKVACAADARRIVSHYERRWLIEEYHKAWKSGGT
                     CVESLRMQTRDNLERMMVIKAFIAVRVLGLRQGGISEETQNDSCEKILTPTEWKLLWV
                     KLEGKPLPSQMPTLKWACLKLAKLGRWHDSKRTGRPGWVVMWDGWFRLQDMVEGYLVM
                     KSLDQEI"
     gene            complement(25642..25953)
                     /locus_tag="ACHJ8C_002052"
     CDS             complement(25642..25953)
                     /locus_tag="ACHJ8C_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO5551911.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQLYRWN
                     Y"
     gene            26116..26212
                     /gene="ffs"
                     /locus_tag="ACHJ8C_002053"
     ncRNA           26116..26212
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHJ8C_002053"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            26332..26685
                     /gene="ybaA"
                     /locus_tag="ACHJ8C_002054"
     CDS             26332..26685
                     /gene="ybaA"
                     /locus_tag="ACHJ8C_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO5551912.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(26727..28277)
                     /gene="pdeB"
                     /locus_tag="ACHJ8C_002055"
     CDS             complement(26727..28277)
                     /gene="pdeB"
                     /locus_tag="ACHJ8C_002055"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO5551913.1"
                     /translation="MRTRHLVSLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDELAQGIITRLQKTPGEHIENNGIIYDILP
                     LPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(28441..28911)
                     /gene="ylaC"
                     /locus_tag="ACHJ8C_002056"
     CDS             complement(28441..28911)
                     /gene="ylaC"
                     /locus_tag="ACHJ8C_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO5551914.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(29027..29578)
                     /gene="maa"
                     /locus_tag="ACHJ8C_002057"
     CDS             complement(29027..29578)
                     /gene="maa"
                     /locus_tag="ACHJ8C_002057"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO5551915.1"
                     /translation="MSTEKEKMIAGELYHSADEALSRDRLRARRLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(29752..29970)
                     /gene="hha"
                     /locus_tag="ACHJ8C_002058"
     CDS             complement(29752..29970)
                     /gene="hha"
                     /locus_tag="ACHJ8C_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO5551916.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(29996..30370)
                     /gene="tomB"
                     /locus_tag="ACHJ8C_002059"
     CDS             complement(29996..30370)
                     /gene="tomB"
                     /locus_tag="ACHJ8C_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO5551917.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         7727 a         7397 c         8294 g         7300 t
ORIGIN      
        1 attagctgaa tcgtgtaaga aaaatagaat tcataaattg cgtcaataat ttccaaacgc
       61 taagccgcac aaaagaacaa atattaataa gcgatgctga tgtttattta gccgtaataa
      121 ttacggcgag tgattactac agctaaataa tattacagat tacatcagat gtgtttttcg
      181 tagcgccaga taatccagca aaccaccaag cacaacgcca gaaagggcga ctaaaacccc
      241 gacttccagc agcgcaggca ggaaagagaa gtgcgtcaaa tccagcgcgt ccatcgtcaa
      301 cagcagtaac cacacggcca acaggctgat gcccgccgcc agtgtccaca tggcaaaggc
      361 ataacctgca ggatattcgg tacgggagat aaagttgtca tttactcgtg tatattcaag
      421 cgtctggcgg caaaccagca gcaaaataag ccctggtacg gcagcggcga cggagaatag
      481 atagaatgtc gaccagccat gtgcttcaac aaaccaaccc gccacggggc cgacataaac
      541 tcgccctaca gcagaaagcg ctgagagcag ggcaaattga gtagcggaaa atgacttatt
      601 acacagcgtc attaacagcg cgacaaaggc tgatgtaccc atcccgccac agaggttttc
      661 gaaaaagacg gctgcaccca tgctgtagag atgcttatca gtaatcgaca gcagccagta
      721 accggcgtta gatgcacctt gtaaaatgcc gaaaatcagc agtgcacgga acagcgacag
      781 gcgctgcatc aaaatcccac cgtacaatgc gccaacaatg gtcgccagta agccaagcgt
      841 tttgttaacc acgcctactt cacccgcatc aaacccgacg ccgcgaatca aaaacgtggt
      901 tgtcaggctc atagcgaatg cgtcgcccag cttatacagc acgataagaa gcaaaataag
      961 ccaggcatta ttgcgaccaa agaaatctcg cagaggtgca acaaccgctt gttccagcgt
     1021 tttgggcaca ggaatggtgt cggtgggttc tggtgcaagc aacgtcgcga taatacaggg
     1081 gatcaacagc gccgccatca accagtacat gccctgccag cccagccatt tatctgccag
     1141 ccacagggct aagccgccgg aaaccagcat ccctaaacgg taacccagca cgctgattgc
     1201 cgcgcccgca ccacgttctt ctgccgggag cacatcggtt ttccacgcat cgaagacaat
     1261 atcctgggag gcagagcaaa aagcgatcac caccgccagt gccgccatcc agcggagctg
     1321 ggtgcctggt tcgagaaaac ccatcgccgc aatggcgact aataacagaa tttgcgtggc
     1381 gagcagccaa ccgcgccgcc gcccgaaaaa tgggggcgtg tagcggtcca tcagcggtga
     1441 ccagaggaat ttaaaaacgt aagcctggcc taccagagag aagaaaccaa tggttttgag
     1501 atcgatattc tcgaccgtca tccaggcctg taaggtgccg gacgtcaggg cgagcggtag
     1561 cccggaagca aaacccagga tcagcaatat ggctgaacgc ggctgttgaa aaatacgtaa
     1621 atattgactg gacatgggcg tctaaaggcc cggcttgcac cgggccagag ggcagaaatt
     1681 aacgcgcgtt ctgcttgatg aattcgtgga tgctggtgtc ctgagacata tcagcgatgg
     1741 tatcggtcag cacactgtta accgcatcgg cgatattttt gttggaggcc tggaatgcac
     1801 cttcaacgtt gtagctggca cgatagtttt tggtcatttt gttgccattc tgcgcggtag
     1861 cgatgatggc gatatccgct ttagtagcga tgttgtagcg cacgttgccc tgggacacgt
     1921 cagcatacag ttggctaacg atgatttgca gattaaccgg gccattcgga ccaaccatgt
     1981 aaccacgcgc ggtcatctgt ttttccagca cttcttgcag caggaaacgc agatcgcggg
     2041 aggcggtcag ggtaacgatt tgattatcgc gggtgacttt tgccagcgcc tgatcggtac
     2101 gctgatcggc accattaatg cttacggtga cgcccatcag gcttggatcc tgctgtggca
     2161 gtgtaatcgt cggggaaact tcaatagttg ttggcggttt tgcgcatcct gcaagcataa
     2221 acagagcaac taacgggaag aggatttttt tgaacatgtt cgggctctca gagactctta
     2281 agcgtgtttg gtaaaaattc ccgccatcat aacattgcca acggcgaggg gaagtgggta
     2341 aggcatgtaa attcatcatg ttgacgaaat aatcgcccct ggtaaaagaa acgctgatgc
     2401 gaggcctgtg tttcaatctt taaatcagta aacttcatac gcttgacgga aaaaccagga
     2461 cgaaacctaa atatttgttg ttaagctgca atggaaacgg taaaagcggc tagtatttaa
     2521 agggatggat gacatctcag cgttgtcgga ggagatattt catgatgata cgtgagcgga
     2581 tagaagaaaa attaagggcg gcgttccaac ccgtattcct cgaagtagtg gatgaaagct
     2641 atcgtcacaa tgtcccagcc ggctctgaaa gccattttaa agttgtgctg gtcagcgatc
     2701 gttttacggg tgaacgtttt ctgaatcgtc atcgaatgat ttacagtact ttagcggagg
     2761 aactctctac taccgttcat gcgctggctc tgcatactta cactattaag gagtgggaag
     2821 ggttgcagga caccgtcttt gcctctcctc cctgtcgtgg agcaggaagc atcgcgtaaa
     2881 aacgcatttg caactgtcgg cgcttttcca gtatgttgct aaagatttta tgaaaaacgg
     2941 cctgcgggcc gttttgtttt gtctggattt tgcgcttttt gcccagcatt cagacgaaaa
     3001 tcgcccggga attgtgaaaa aatacgcgac agcgcgcaat aaccgttctc gactcataaa
     3061 agtgatgccg ctataatgcc gcgtcctatt tgaatgcttt cgggatgatt ctggtaacag
     3121 ggaatgtgat tgattataag aacatcccgg ttccgcgaag ccaacaacct gtgcttgcgg
     3181 ggtaagagtt gaccgagcac tgtgattttt tgaggtaaca agatgcaagt ttcagttgaa
     3241 accactcaag gccttggccg ccgtgtaacg attactatcg ctgctgacag catcgagacc
     3301 gctgttaaaa gcgagctggt caacgttgcg aaaaaagtac gtattgacgg cttccgcaaa
     3361 ggcaaagtgc caatgaatat cgttgctcag cgttatggcg cgtctgtacg ccaggacgtc
     3421 ctgggcgacc tgatgagccg taacttcatt gacgccatca ttaaagaaaa aatcaatccg
     3481 gctggcgcac cgacctatgt tccgggcgaa tacaagctgg gtgaagactt cacctactct
     3541 gtagagtttg aagtttatcc ggaagttgaa ctgcagggtc tggaagcgat cgaagttgaa
     3601 aaaccgatcg ttgaagtgac cgacgctgac gttgacggca tgctggatac tctgcgtaaa
     3661 cagcaggcaa cctggaaaga aaaagacggc gctgttgaag cagaagaccg tgtgaccatc
     3721 gacttcaccg gttctgtaga cggcgaagag tttgaaggcg gtaaagcgtc tgatttcgta
     3781 ctggcgatgg gtcagggtcg tatgatcccg ggctttgaag acggtatcaa aggccacaaa
     3841 gctggcgaag agttcaccat cgacgtgacc ttcccggaag agtaccacgc agaaaacctg
     3901 aaaggtaaag cagcgaaatt cgctatcaac ctgaagaaag ttgaagagcg tgaactgccg
     3961 gaactgactg cagaattcat caaacgtttc ggcgttgaag atggttccgt agaaggtctg
     4021 cgcgctgaag tgcgtaaaaa catggagcgc gagctgaaga gcgccatccg taaccgcgtt
     4081 aagtctcagg cgatcgaagg tctggtaaaa gctaacgaca tcgatgtacc ggctgcgctg
     4141 atcgacagcg aaatcgacgt tctgcgtcgc caggctgcac agcgtttcgg tggcaacgaa
     4201 aaacaagctc tggaactgcc gcgcgaactg ttcgaagaac aggctaaacg ccgcgtagtt
     4261 gttggcctgc tgctgggcga agttatccgc accaacgagc tgaaagctga cgaagagcgc
     4321 gtgaaaggcc tgatcgaaga gatggcttct gcgtacgaag atccgaaaga agttatcgag
     4381 ttctacagca aaaacaaaga actgatggac aacatgcgca atgttgctct ggaagaacag
     4441 gctgttgaag ctgtactggc gaaagcgaaa gtgactgaaa aagaaaccac tttcaacgag
     4501 ctgatgaacc agcaggcgta atttacgtag cataacgcgc taaattcgca caaaggcccg
     4561 tcaccgccag gtggtgggct tttttttgtc atgaattttg catggaaccg tgcgaaaagc
     4621 ctctttcggt gttagcgtaa caacaaaaga ttgttatgct tgaaatatgg tgatgccgta
     4681 cccataacac agggactagc tgataatccg tccataaggt tacaatcggt acagcaggtt
     4741 ttttcaattt tatccaggag acggaaatgt catacagcgg cgaacgagat aactttgcac
     4801 cccatatggc gctggtgccg atggtcattg aacagacctc acgaggtgag cgctcttttg
     4861 atatctattc tcgtctactt aaggaacgcg tcatttttct gactggccag gttgaagatc
     4921 acatggctaa cctgattgtg gcgcagatgc tgttcctgga agcagaaaac ccagaaaaag
     4981 atatctatct gtacattaac tctccaggcg gggtgattac tgccgggatg tctatctatg
     5041 acaccatgca gtttatcaag cctgatgtca gcaccatctg tatgggccag gcggcctcga
     5101 tgggtgcttt cttgctgact gcaggggcaa aaggtaaacg tttctgcctg ccgaattcgc
     5161 gcgtgatgat tcaccagccg ttgggcggct accagggcca ggcgaccgat atcgaaattc
     5221 atgcccgtga aattctgaaa gttaaagggc gcatgaatga acttatggcg cttcatacgg
     5281 gtcaatcatt agaacagatt gaacgtgata ccgagcgcga tcgcttcctt tccgcccctg
     5341 aagcggtgga atacggtctg gtcgattcga ttctgaccca tcgtaattga tgccagaggc
     5401 gcaactgtgc cgctatactt atccagggcg gcacaacgct gtaagcggct tgcgcctgag
     5461 aatggcattt gcgtcgtcgt gtgcggcaca aagaacaaag aagaggtttt gacccatgac
     5521 agataaacgc aaagatggct caggcaaatt gctgtattgc tctttttgcg gcaaaagcca
     5581 gcatgaagtg cgcaagctga ttgccggtcc atccgtgtat atctgcgacg aatgtgttga
     5641 tttatgtaac gacatcattc gcgaagagat taaagaagtt gcaccgcatc gtgaacgcag
     5701 tgcgctaccg acgccgcatg aaattcgcaa ccacctggac gattacgtta tcggtcagga
     5761 acaggcgaaa aaagtgctgg cggtcgcggt atacaaccac tacaaacgtc tgcgcaacgg
     5821 agataccagc aatggcgtcg agttgggcaa aagtaacatt ctgctgatcg gtccgaccgg
     5881 ttccggtaaa acgctactgg ccgaaacgct ggcgcgcctg ctggacgttc cgttcaccat
     5941 ggctgacgca accacgctga ccgaagccgg ttatgtgggc gaagacgttg aaaacatcat
     6001 tcagaagctg ttgcagaagt gcgattacga cgtacagaaa gcgcagcgcg ggattgtcta
     6061 catcgatgaa atcgacaaga tttctcgtaa gtcagacaac ccgtccatta cccgagacgt
     6121 ttccggtgaa ggcgtacagc aggcactgtt gaaactgatc gaaggtacgg tagctgctgt
     6181 tccgccgcaa ggtgggcgta aacatccgca gcaggaattc ttgcaggttg atacctctaa
     6241 gatcctgttt atctgtggcg gtgcgtttgc cggtctggat aaagtgattt cccatcgtgt
     6301 agaaaccggc tccggcattg gttttggcgc gacggtaaaa gcgaagtccg acaaagcaag
     6361 cgaaggcgag ctgctggcgc aggttgaacc ggaagatctg atcaagtttg gtctgatccc
     6421 tgagttcatt ggtcgtctgc cggttgtcgc aacgttgaat gaactgagcg aagaagctct
     6481 gattcagatc ctcaaagagc cgaaaaacgc cctgaccaag cagtatcagg cgctgtttaa
     6541 tctggaaggc gtggatctgg aattccgtga cgaggcgctg gatgctatcg ctaagaaagc
     6601 gatggcgcgt aaaaccggtg cccgtggcct gcgttccatc gtagaagccg cactgctcga
     6661 taccatgtac gatctgccgt ccatggaaga tgtcgaaaaa gtggttatcg acgagtcggt
     6721 aattgatggt caaagcaaac cgttgctgat ttatggtaag ccggaagcgc aacaggcatc
     6781 tggtgaataa ttaaccattc ccatacaatt agttaaccaa aaagggggga ttttatctcc
     6841 cctttaattt ttcctctatt ctcggcgttg aatgtggggg aaacatcccc atatactgac
     6901 gtacatgtta atagatggcg tgaagcacag tcgtgtcatc tgattacctg gcggaaatta
     6961 aactaagaga gagctctatg aatcctgagc gttctgaacg cattgaaatc cccgtattgc
     7021 cgctgcgcga tgtggtggtt tatccgcaca tggtcatccc cttatttgtc gggcgggaaa
     7081 aatccatccg ttgtctggaa gcggcgatgg accatgataa aaaaattatg ctggtcgcgc
     7141 agaaagaagc ttcaacggat gagccgggtg taaacgatct tttcaccgtc gggaccgtgg
     7201 cctctatatt gcagatgctg aaactgcctg acggcaccgt caaagtgctg gtcgaggggt
     7261 tacagcgcgc gcgtatttct gcgctctctg acaatggcga acacttttct gcgaaggcgg
     7321 agtatctgga gtcgccgacc attgatgagc gggaacagga agtgctggtg cgtactgcaa
     7381 tcagccagtt cgaaggctac atcaagctaa acaaaaaaat cccaccagaa gtgctgacgt
     7441 cgctgaatag catcgacgat ccggcgcgtc tggcggatac cattgctgca catatgccgc
     7501 tgaaactggc tgacaaacag tccgttctgg agatgtccga cgttaacgaa cgtctggaat
     7561 atctgatggc aatgatggaa tcggaaatcg atctgctgca ggttgagaaa cgcattcgca
     7621 accgcgttaa aaagcagatg gagaaatccc agcgtgagta ctatctgaac gagcaaatga
     7681 aagctattca gaaagaactc ggtgaaatgg acgacgcgcc ggacgaaaac gaagccctga
     7741 agcgcaaaat cgacgcggcg aagatgccga aagaggcaaa agagaaagcg gaagcagagc
     7801 tgcagaagct gaaaatgatg tctccgatgt cggcagaagc gaccgtagtg cgtggatata
     7861 tcgactggat ggtacaggtg ccgtggaatg cgcgtagcaa ggtcaaaaaa gacctgcgtc
     7921 aggcgcagga aatccttgat accgaccatt atggtctgga gcgcgtgaaa gatcgtatcc
     7981 ttgagtatct tgcggttcaa agccgtgtca acaaaatcaa gggaccgatc ctctgcctgg
     8041 tagggccgcc gggggtaggt aaaacctccc tgggtcagtc catcgccaaa gccaccgggc
     8101 gtaaatatgt ccgtatggcg ctgggcggcg tgcgtgatga agcggaaatc cgtggtcacc
     8161 gccgtactta catcggttct atgccgggta aactgatcca gaaaatggcg aaagtgggcg
     8221 tgaaaaaccc gctgttcctg ctcgatgaga tcgacaaaat gtcttctgac atgcgtggtg
     8281 atccggcctc cgcactgctt gaagtgctgg atccagagca gaacgtagcg ttcagcgacc
     8341 actacctgga agtggattac gatctcagcg acgtaatgtt tgtcgctacg tcgaactcca
     8401 tgaacattcc ggcaccgctg ctggatcgta tggaagtgat tcgcctctcc ggttataccg
     8461 aagatgaaaa actgaacatc gccaaacgtc acctgctgcc gaagcagatt gaacgtaatg
     8521 cactgaaaaa aggtgagctg accgtcgacg atagcgccat tatcggcatt attcgttact
     8581 acacccgtga ggcgggcgtg cgtggtctgg agcgtgaaat ctccaaactg tgtcgcaaag
     8641 cggtaaagca gttactgctc gataagtcat taaaacatat cgaaattaac ggcgataacc
     8701 tgcatgacta cctcggtgtt cagcgtttcg actatgggcg cgcggataac gaaaaccgtg
     8761 tcggccaggt aaccggtctg gcgtggacgg aagtgggcgg tgacttgctg accattgaaa
     8821 ccgcgtgcgt tccggggaaa ggtaaactga cctataccgg ttcgctcggc gaagtgatgc
     8881 aggagtctat tcaggcggcg ttaacggtgg ttcgtgcgcg tgcggaaaaa ctggggatca
     8941 accctgattt ttacgaaaaa cgtgacatcc acgtccacgt accggaaggt gcgacgccga
     9001 aagatggtcc gagtgccggt attgccatgt gcaccgcgct ggtttcttgc ctgaccggta
     9061 acccggttcg tgccgatgtg gcaatgaccg gtgagatcac tctgcgtggt caggtactgc
     9121 cgatcggtgg tttgaaagaa aaactactgg cagcgcatcg cggcgggatt aaaacagtgc
     9181 taattccgtt cgaaaataaa cgcgatctgg aagagattcc tgacaacgta attgccgatc
     9241 tggacattca tcctgtgaag cgcattgagg aagttctgac tctggcgctg caaaatgaac
     9301 cgtctggcat gcaggttgtg actgcaaaat agtgacctcg cgcaaaatgc actaataaaa
     9361 acagggctgg caggctaatt cgggcttgcc agcctttttt tgtctcgcta agttagatgg
     9421 cggatcgggc ttgcccttat taaggggtgt tgtaagggga tggctggcct gatataactg
     9481 ctgcgcgttc gtaccttgaa ggattcaagt gcgatataaa ttataaagag gaagagaaga
     9541 gtgaataaat ctcaattgat cgacaagatt gctgcagggg ctgatatctc taaagctgcg
     9601 gctggccgtg cgttagatgc tattattgct tccgtaactg aatctctgaa agaaggggat
     9661 gatgtagcac tggtaggttt tggtactttt gccgttaaag agcgtgctgc ccgtactggc
     9721 cgcaacccgc agaccggtaa agagatcacc atcgctgccg ctaaagtacc gagcttccgt
     9781 gcaggtaaag cactgaaaga cgcggtaaac taagcgttgt ccccagtggg gatgtgacga
     9841 agttcaaggg cgcatctact gatgtgcctt ttttatttgt attcggtgac tttctgcgtc
     9901 ttgtgggctg acaattgccc ccgtttcttg tcacaatagg cctttgcgcg catcgatacg
     9961 ttgcgtgagg tacacagtca tctacagcgg agtgttgtta caccatgatg gacagcttac
    10021 gcacggctgc aaacagtctc gtgctcaaga ttattttcgg tatcattatc gtgtcgttca
    10081 tattgaccgg cgtgagtggt tacctgattg gcggaggcaa taactacgcc gcaaaagtga
    10141 atgaccagga aatcagccgt gggcaattcg agaacgcctt caacagcgag cgtaatcgca
    10201 tgcagcaaca gctgggcgat caatactccg agctggcagc gaacgaaggc tatatgaaaa
    10261 ccctgcgtca acaggtgctg aatcgtctga tcgacgaggc gcttctggat cagtacgctc
    10321 gtgagctgaa actgggtatc agcgatgagc aggttaaaca ggcgattttc gcgaccccag
    10381 ccttccaggt tgacggcaaa tttgataaca gccgctataa cggtatcctc aaccagatgg
    10441 ggatgaccgc cgatcagtac gcccaagcgc tgcgtaacca gctcacaacc caacagctga
    10501 ttaacggcgt tgccggtacc gattttatgc tgaaaggtga aaccgacgag ctggcggcac
    10561 tggtagctca acaacgcgtg gtgcgtgaag cgactatcga tgttaacgcg ctggcggcga
    10621 agcagcctgt gaccgaacag gaaattgcca gctactacga acaaaacaaa aacaatttca
    10681 tgacgccgga acaattccgc gtgagttaca tcaagctgga tgccgcgacg atgcagcaac
    10741 cggttagcga tgcggatatc cagagctact acgatcagca tcaggatcaa ttcacccagc
    10801 cgcagcgtac ccgttacagc atcatccaga ccaaaactga agatgaagcg aaagcggtac
    10861 ttgatgagct gaataagggc ggtgattttg ctgcattagc caaagaaaaa tctgccgata
    10921 ttatctctgc ccgcaacggc ggtgatatgg gttggttaga agatgccact atcccggacg
    10981 aactgaaaaa tgcgggtctg aaagaaaaag gccaactgtc tggtgttatc aaatcttcgg
    11041 tcggtttcct gattgtgcgt ctggacgaca ttcagccagc gaaagtgaaa tcgttagacg
    11101 aagtacgtga cgacattgcg gcgaaagtga aacacgaaaa agccctcgat gcgtactacg
    11161 cgctgcagca gaaagtgagc gatgcggcaa gcaacgacac cgagtctctg gccggtgcag
    11221 agcaagctgc cggcgttaaa gccactcaga cgggttggtt cagcaaagat aacctgccgg
    11281 aagagttgaa cttcaagcca gttgccgacg ctatcttcaa cggcggtctg gtaggtgaaa
    11341 acggcgcgcc gggcatcaac tctgacatca tcaccgtaga tggcgaccgc gcattcgtgc
    11401 tgcgcatcag cgagcacaaa ccggaagcgg tgaaaccgtt ggcagatgtt caggaacaag
    11461 ttaaggcact ggttcagcac aacaaagctg aacaacaggc gaaagtggat gctgagaaac
    11521 tgctggttga tttgaaagcc ggcaaaggtg cggaagctat gcaggctgcc ggtctgaaat
    11581 ttggcgagcc gaaaacctta agtcgttccg gtcgtgaccc gattagccag gctgcgtttg
    11641 cactgccgct gccagcgaaa gacaaaccga gctacggtat ggcgaccgat atgcaaggta
    11701 atgtagttct gctggcgctg gatgaagtga agcaaggttc aatgccggaa gatcagaaaa
    11761 aagcgatggt gcagggtatc acccagaaca acgcacaaat cgtctttgaa gctctgatga
    11821 gtaacctgcg taaagaggcg aaaatcaaaa ttggcgatgc gctggaacag caataatcct
    11881 gaagccgcct cgcaaaaaaa tgctttgcag ctgtaacaag aaaaggtcgc tttcgcggcc
    11941 ttttccattt ctgaacattg ccatttgttt actgttttca ctgccgttaa ggtgattcca
    12001 ctgttaacaa acaaggagaa aacagtatga aacacggaat taaagcactg ctcattaccc
    12061 tgtccctggc ctgtgccgga atgtctcata gtgctctggc ggcagctcct gcggcaaaac
    12121 cgacaacggt agaaaccaaa gcggaagctc ctgcagcaca aagtaaagca gcagttccgg
    12181 cgaaagccag tgacgaagaa ggcacccggg tcagcattaa taatgccagc gcggaagagt
    12241 tagcccgcgc gatgaatggc gttggcctga agaaagcgca ggcgattgtc agttatcgcg
    12301 aagagtacgg tccgtttaaa acggtggagg atctaaagca ggtgccgggg atgggcaatt
    12361 cgctggtgga acgtaatctg gcggtattaa ccctgtaatt aatttgcata gtggcaattt
    12421 tttgccagac tgaagaggtc ataccagtta tgacctctgt acttataaca gttcttataa
    12481 caacaacgta aggttattgc gctatgcaaa cacaaatcaa agttcgtgga tatcatctcg
    12541 acgtttacca gcacgtaaac aacgcccgct accttgaatt tctcgaagaa gcccgctggc
    12601 atgggttgga aaatagcgac agttttcact ggatgacggc ccataacatc gccttcgtcg
    12661 tggtcaatat caatattaac tatcgccgcc cggcggtatt aagcgacctg ttaaccatta
    12721 ccagtcagtt gcagcaatta aacggtaaaa gtggcatctt aagccaggtc attacactgg
    12781 agccggaagg acaggtggta gcggatgcgc ttattacgtt tgtttgtatt gatcttaaaa
    12841 cgcagaaagc attagctctg gaaggggaat tgcgcgaaaa gctggagcag atggttaagt
    12901 aaacgttttg tggtgccgga tgctcaagcc gcatccggcg acacccggaa taattacttc
    12961 aacccggttt tctgcttcat cgctgccatc accgtcggtt tatcggccag ataatgattc
    13021 aaaccgttgg cacgtaaatt acacgccgca caatgaccgc aaccgtcgcc tttaatgccg
    13081 ttataacagg tcagcgtttc gttacggact aaatccagct tgccgtaata atctgccagc
    13141 gcccaggttt ccgctttatc aatccacatc agcggcgttt caaaacgaat atctttcgcc
    13201 atgcccaaac tgacggcatg gtttagtgct ttcacaaact catcgcggca atccgggtag
    13261 ccggagaaat ccgtttcgca gacgccggta attacggctt ctgcttttac ctgatacgca
    13321 tatattgccg ccagcgtcag gaacaaaata ttacgccctg ggacaaacgt attcgggata
    13381 ccatcggctt caggttcata atcaggcacc ggaatgctgt cacgcgtcag gctgctgacc
    13441 gccagctcgt tgagcagcgt tacatcaagc accttatgcg cgcgtgcccc cagtttcagc
    13501 gccagttcgc gtgccacgtc gatttctgcg cgatggcgtt gaccgtaatc gaacgtcacg
    13561 caatggactt catcatattg ttgtaatgcc tgcaccagac aggtggtgga atcctggcct
    13621 ccactgaaca caacgacagc acgtttcata gataatcctg cttgaacaat aaaagcgtta
    13681 tggtaacgcc tgcgattaac ccggaccagc tattcattgc gacggcgcgg gaagccaggc
    13741 ttcggtaaat tcaaaccagc cgcgcggtgt cagtcgcaca ccgttcacgc cgggaggggc
    13801 gctaatgcga tagtgatagt tgaacagcgg cgtcagcgtc gcatctgcca ttaactggct
    13861 aaaaaccgct ttcagggcat taaatcggtt ttcctcatca ggcattactt gcactgcgtc
    13921 cagtgtcgat tgcaaatgtg catatgctgg agcgtcgaaa acatgtggcc acagtggatc
    13981 gcaacgcagc cattgctcca gggtatattc cggtgcttcg ccaattaatc tgtcgcccat
    14041 catgaggtct gcgtgtgcca gtagggtcgt atcgtcccag ttttttgcgt tatgaaaaat
    14101 aattgtaagt tcacaacctt ccgctgccag tgtcgcctgt aggcgttctg ccatggtatg
    14161 aagttctatc ggtaggtgat aaaccagcgt caaggttttc ggtagtttaa cttcatccgg
    14221 tacttgccaa tgcggaatag tccagcctgg cagtaatgca tgactggcgg tgatcaggtt
    14281 ttctccgact tctaacgttt gtaataaacc ggattgatga ataatggaga tcacttttcg
    14341 cgcctgccag agggagagtc ggggactttt gcgcaacgtt aaatagcaaa aacctaaact
    14401 gatgccacta ctgacctggc tgacccgttg cagctcctcc ggtttgccga tggtgatttg
    14461 cacgggatgc cgacaactgg ttcccaaatc tttttcgaaa agtggcggag ttatccagta
    14521 ctcaaccgct ttaagcagcg gatgacgtaa atggtaataa tcatggcttt ccaggcgcac
    14581 cagctccgct gtgaattgtg ttaagcgaaa aggaccagtg ccgatcagtg ggaattgcgg
    14641 atgcgccaga tggctgcaat agctcgccag tcggtgcgca agccagtaat cggggagatg
    14701 taaaaagaag gtcagacact gcggatgggt gacttcaata cgcttcacgc taataaataa
    14761 ttgatccagt gctggcagtt gtaacagcat caataatcgc tggtgtaagt gtgaggcttt
    14821 tactgcatcg ccgttatgcc agtgtagcgt tgaacgaaga taaaagtccc agcgtaaccc
    14881 gtcagtagag gtttcccagt gatgtgctaa atcgccaatc gggcgctgag tattattatc
    14941 gaagcgggtc aggccggaaa atatctgccc ggcgagatgc tgctcagcac ggccgggcaa
    15001 aaagcccggt tgtagcggtt cgagcgggcg atagtaggga atacgcaacg tgggcgtatc
    15061 gttttgccat tgtccgccca taaacggctg taacagagtg cgcagctcac ctggggccag
    15121 ttgcgccagc tccagcacat cttgctgctt tccggtttcc agcgcctgtt ccatcatcgc
    15181 attgcgtagc gattctggcg tgaccagaaa gcgtagttga ccgcgctttc cgcgtcctga
    15241 ctgcgcctgc cactccagcc atcccgcctc ctgtgcctgg cgtaacagcg tacgaacatg
    15301 gcgttcgctg caaaaacagc gttcggccag ttcgctgacg gtgacggttt gcggctttcc
    15361 ggcggaaggt tgccacagac gttgatactg gttaagacgg ttgagcagtc gcatataaac
    15421 ccggaacaat attatttaac tattcactat tacttccgta tatatcaggt gatactcaat
    15481 caccattaac cgtgtcacag agtggagaaa gaaatggctc gtctggcagc atttgatatg
    15541 gatggcactt tattgatgcc tgaccatcat ttaggtgaga aaaccctctc tactttggcg
    15601 cggctgcgtg aacgcgacat taccctcact tttgccacgg ggcgtcatgc gctggagatg
    15661 cagcatattc tcggggcgct atcgctggat gcatatttga ttaccggcaa cggaacgcgc
    15721 gtgcattctc tggaaggtga acttttacat cgtgatgatt tacctgcgga tgtcgcggag
    15781 ctggtgctgt atcagcaatg ggatacccga gccagcatgc atatcttcaa tgacgatggt
    15841 tggtttaccg ggaaagagaa ccctgcgttg ttgcaggcat ttgtctatag cggttttcgt
    15901 tatcagataa tcgatgtcaa aaaaatgcca ctcggcagcg tcaccaagat ctgcttctgt
    15961 ggcgatcacg acgatcttac acgcttgcag atccagctac acgaagcatt aggcgagcgt
    16021 gcacatttat gtttttccgc cacggattgc ctcgaagtgc tgccggtggg ctgcaataaa
    16081 ggcgctgcat tgacggtgct gacccaacat ttaggtttat cgttgcgcga ttgcatggcc
    16141 tttggtgatg cgatgaacga tcgcgaaatg ttaggcagcg tcggtagcgg atttattatg
    16201 ggcaatgcga tgccgcaact gcgcgcggag ctcccgcatt taccggtgat tggacattgc
    16261 cgaaatcagg ctgtctctca ctatttgacg cactggctgg actatccaca tctaccttat
    16321 tcccccgaat aacgagatcc cttccagcac cgggcaattg cccggttttt tttgcgttga
    16381 atttgtcatt ttgtgccgtg gtgtttaaac cgcacagaat aaattgtcgc gatttcacct
    16441 ttaaaataga attaaaagag aaaaaaattc tccgtggaag ggctatgtta gataaaattg
    16501 accgtaagct gctggcctta ctacagcagg attgcaccct ctctttgcag gcactggctg
    16561 aagccgttaa tctgacaact accccctgct ggaagcgtct gaaacggctg gaggacgacg
    16621 gtattcttat cggcaaagtc gccctgctgg atccggaaaa aataggcctc ggcctgaccg
    16681 cctttgtgct gataaaaacg caacatcaca gcagcgaatg gtattgccgc tttgtcacgg
    16741 tggttaccga aatgccagaa gtgctggggt tctggcgcat ggccggtgaa tacgattatc
    16801 tgatgcgcgt ccaggttgcc gacatgaaac gctacgacga gttttataag cgtctggtaa
    16861 acagtgtacc ggggctgtcg gacgtcactt ccagcttcgc gatggaacag attaaataca
    16921 ccacttcttt acccatcgaa taaatatcca gaatcaagtc aggacacaac gcgtgcgatt
    16981 atttgctcaa ttaagctggt atttccgtcg ggaatggcgt cgctatctcg gggctgtcgc
    17041 cttgcttgtc attatcgcga tgctgcaact ggttccgcca aaagtggttg gtattgttgt
    17101 tgatggcgtg acagaacaac actttactac cgggcagatc ctgatgtgga tcgccaccat
    17161 ggtgctgatt gccgttgtgg tttatctcct gcgttacgtc tggcgcgtat tgctgtttgg
    17221 tgcgtcttat caactggctg ttgaactgcg tgaagattat taccgccagc taagccggca
    17281 gcatcctgag ttttacctgc gtcatcgcac gggtgacctc atggctcgtg cgaccaatga
    17341 cgtcgatcgc gtcgtgtttg ccgccggaga aggggtgctg acgctggtgg attcactggt
    17401 aatgggctgc gctgtgttga ttatgatgtc tacgcaaatt agctggcagt tgaccttatt
    17461 tgccctgttg ccgatgccag tgatggcgat catgatcaag cgcaacggcg atgccttgca
    17521 tgaacgcttt aagctggcac aggcggcatt ttccagtctt aatgaccgca cccaggaaag
    17581 cctcaccagt atccgcatga tcaaagcctt tggtctggaa gatcgtcagt cggcgttatt
    17641 tgccgcggat gccgaagata ccggcaaaaa aaacatgcgg gtggcgcgta ttgatgctcg
    17701 tttcgacccg accatctata tcgcgattgg tatggcgaac ttgctggcga ttggcggcgg
    17761 tagttggatg gtggtacagg gcagtttaac gctgggccag ctcaccagtt ttatgatgta
    17821 tttaggtctg atgatttggc caatgctggc gctggcatgg atgtttaaca ttgtggaacg
    17881 tggcagcgcg gcgtatagcc gaattcgtac catgctggcc gaagcgccag tcgttattga
    17941 tggtagtgat aaggtgccag aaggtcgtgg tgaactggat gtgaatattc gccagtttac
    18001 gtatccgcag actgaccatc ctgcgctgga aaacgtcaat ttcgccctga aacccggtca
    18061 gatggtaggt atttgcgggc cgactggttc cggcaaaagt accctgttgt cgctcattca
    18121 gcgtcatttc gacgtcagcg agggggatat ccgctttcat gatattcctc tgacgaagtt
    18181 acaactcgat agctggcgta gccgcctggc ggtagttagc cagacaccgt tccttttttc
    18241 cgacaccgta gcgaacaaca tcgcgttggg ttgcccgaat gccacccagc aagagattga
    18301 gcatgtcgcg cggttagcca gcgtgcatga cgatattttg cgtttaccgc aaggttacga
    18361 tacagaagtg ggcgagcgcg gcgtgatgct ttccggtggg caaaaacagc gtatctccat
    18421 tgctcgtgcg ttattagtca acgcggaaat cctcatcctt gatgatgcgc tttcggcagt
    18481 agacggacgc acagagcacc agatcctgca taacctgcgt cagtgggggc agggaagaac
    18541 ggtaatcatc agtgcccatc gcctttctgc actgacggaa gccagtgaaa ttattgtgat
    18601 gcagcacgga catatcgccc agcgtggcaa tcatgatgag ctggcgcaac aaagtggctg
    18661 gtatcgcgat atgtatcgct atcaacaact ggaggcggcg ctcgacgacg ctccggaaat
    18721 tcgcgaggag gccgtcgatg cgtagtttta gccaactgtg gccgactctc aagcgtctgt
    18781 tagcgtacgg ttcaccgtgg cgtaaaccgt tggggattgc ggtcctgatg atgtgggttg
    18841 cggcggcggc agaagtcagt gggccgctgc ttatcagcta ttttatcgac aatatggtag
    18901 cgaaaaataa cctgccattg aaagtggttg cagggctggc ggcggcgtat gttgggctgc
    18961 aactgtttgc agccgggcta cattacgcgc agtcgctgct gtttaatcgg gcggcagtag
    19021 gcgtagtgca acagttgcgt accgacgtga tggatgctgc gttacgccag ccattaagcg
    19081 agtttgatac ccaacccgtc gggcaggtga tttcccgcgt cactaatgac actgaagtga
    19141 tccgcgatct ctacgttacc gtagtggcaa ctgtcctgcg tagcgccgca ctggtgggcg
    19201 cgatgctggt ggcgatgttc agcctcgact ggcgaatggc gctggtggcg ataatgattt
    19261 tcccggtggt gctggtggta atggtgatat atcagcgtta cagcacgccg attgtccgtc
    19321 gtgtgcgcgc ttatctggcg gatatcaacg acggctttaa cgaaatcatc aatggcatga
    19381 gcgttatcca gcagttccgt cagcaggcgc gatttggcga acgtatgggt gaggctagtc
    19441 gttcacacta tatggcgagg atgcaaaccc tgcgcctcga cggttttctg ctgcgtccgc
    19501 tgctgagtct gttttcatcg ctcattcttt gtggcttgtt gatgctgttt ggcttctccg
    19561 ccagcggcac cattgaagtg ggcgtgttgt atgcgttcat cagttatttg gggcgactta
    19621 acgaaccctt aatcgaactg accacgcagc aggcgatgct gcaacaggct gttgttgctg
    19681 gtgagcgcgt ttttgagctg atggacggac cgcgccagca atatggcaat gatgatcgcc
    19741 cgttgcagag cggcaccatc gacgtcgata acgtgtcatt tgcttatcgc gatgacaatc
    19801 tggtgctaaa gaacattaat ctctctgtgc cttcgcgcaa ctttgtagcg ctggtcgggc
    19861 ataccggcag tggcaaaagc accctcgcca gtttattgat gggctattac ccgctaacgg
    19921 aaggtgagat tcgccttgat ggtcgtccat taagtacgct aagtcacagc gcgctgcgcc
    19981 agggcgtggc aatggtgcag caagatccgg tggtgctggc ggataccttc ctcgccaacg
    20041 tgacgctggg acgtgatatc tctgaagaac gcgtctggca ggcgctggaa accgtgcagc
    20101 tggcggagct ggcgcgtagc atgagtgatg gtatttacac gctgcttggc gagcagggga
    20161 ataatctctc agtcgggcaa aagcaactgc tggcactggc gcgcgtgctg gtggagacgc
    20221 cgcaaatcct gatccttgat gaggcaaccg ccagcattga ctccgggact gaacaggcga
    20281 ttcaacatgc tctggcggcg gtgcgtgaac atactacgct tgtggtgatt gctcaccgct
    20341 tatcgactat tgttgatgcc gacaccattc tggtgcttca tcgtgggcaa gccgtggagc
    20401 agggcactca ccagcaactg ctggcggccc agggacgata ctggcagatg tatcaactgc
    20461 aacttgcggg cgaagagctg gcagccagcg tgcgtgaaga ggaatcattg agcgcctgaa
    20521 tagcgcaata tttcatcgtt ggtgcaaaaa tgtaacgcac tgtgcactgt catagtgcgt
    20581 tttcattttc aatcttctta acttcctgct ctctttctcg tttttcattt ctggcacacc
    20641 gcttgcaata ccttcttcgt gtagcagaac cattaccgaa ttctgaccgg aggggatcta
    20701 tgaagctggt gaccgtgata atcaaaccat tcaagctgga agacgttcgt gaagcgttat
    20761 cttccattgg tattcagggc ctgaccgtca ccgaagtgaa aggtttcggg cgtcagaaag
    20821 ggcatgccga gctgtaccgg ggggcggaat acagcgtcaa cttcctgcca aaagtaaaaa
    20881 ttgatgtggc gattgctgat gaccaactcg atgaagtgat cgatatcgtc agcaaagcgg
    20941 cttacaccgg aaaaattggc gacggcaaaa tcttcgtcgc tgaattgcaa cgcgtcattc
    21001 gtattcgtac cggcgaagcc gacgaagcgg cgctgtaatc tctggcacac agcaacagga
    21061 acgaaaaatg aagatagcaa cgataaaaac tgggcttact tcactggcga tgctgccggg
    21121 actggtaatg gctgcacctg cggtggccga taaagccgac aatgcgttta tgatgatttg
    21181 tactgcgctg gtgctgttta tgaccgttcc ggggattgcc ctgttttatg gagggctgat
    21241 tcgtggcaaa aacgtgctgt cgatgctgac gcaggtgacc gtgacgtttg cactggtctg
    21301 tattctctgg gtggtttacg gctactcgct ggcgtttggc gaaggcaaca acttcttcgg
    21361 caacattaac gggttgatgc tgaaaaacat tgaactgacg gcggtgatgg gcagtattta
    21421 tcagtatatc cacgtggcgt ttcagggatc gtttgcctgc attaccgtag gcttgatagt
    21481 tggggcgctg gcggaacgaa tccgcttctc agctgtgttg atttttgtgg tggtatggct
    21541 gacgctctct tacattccga ttgcgcatat ggtctggggc ggcggtttgc tggcttctca
    21601 cggggcactg gatttcgcgg gtggcacggt ggtgcacatt aacgccgcaa tcgccggtct
    21661 ggtgggcgcg tatctgatag gcaaacgcgt gggcttcggt aaagaggcgt ttaaaccgca
    21721 caacctgccg atggtcttca ccgggactgc tattctctat atcggttggt ttggctttaa
    21781 cgccgggtca gcgggcacgg cgaatgaaat cgcggcactg gcatttgtga atactgtggt
    21841 cgcaactgcg gcggcaattc ttggctggat cttcggtgaa tgggcgctgc gtggtaagcc
    21901 ttcactgctg ggagcgtgtt ctggcgcgat tgccggtctg gtcggcgtga cgccagcctg
    21961 tggttacatt ggggttggcg gcgcgttgat tatcggcgtg gtagctggtc tggcgggctt
    22021 gtggggcgtt accatgctca aacgcttgct gcgggtggat gatccctgcg atgtcttcgg
    22081 tgtgcacggc gtttgtggta ttgtcggctg tatcatgacc gggatttttg ccgccagctc
    22141 gctgggcggc gtgggcttcg ctgaaggtgt gacgatgggc catcagttgc tggtacagct
    22201 ggaaagcatc gccattacga ttgtctggtc tggtgtggtg gcatttatcg gctacaaact
    22261 ggcggatttg acggttggtc tgcgtgtacc ggaagagcag gagcgagaag ggctggacgt
    22321 caacagccac ggcgagaatg cctataacgc gtaacaagca ctgcaaaaaa cagccggacg
    22381 gttttcacct ccggctattt ttttaattgt gattacgcat caccccttcc tgaacggtcg
    22441 aggcaaccag cacgccgtcc tgggtataaa actcaccgcg cacaaagccg cgtgcgctgg
    22501 acgccgaggt gctttccacg ctatacagca gccattcatt caaattaaac gggcgatgga
    22561 accacatgga atggtcaatg gtggcaatct gaatccccgg ttcgagaaaa ccgatgccgt
    22621 gcggctgtag agctaccggc aggaaattaa gatcagaagc ataaccgagc agatactgat
    22681 gaacgcgcag atcatccggc acgctaccat ttgcgcggat ccacacctga cgatgcggtt
    22741 ctgcgacgtg acctttcagt gggttatgaa actccaccgg acggacttcc agcggacgat
    22801 cgcagatgaa tttatctttc agcactggcg gcagcaggtg cgccagcgat tgggcgattt
    22861 gcgtttctga aggcaggcca tcaggcgctg gcgcggacgg cattgttttt tgatgttcga
    22921 aacccgcttc tggtgcctgg aaagaggcag tcatataaaa aatcggtttg ccgttttgaa
    22981 tagcagaaac ccggcgggcg ctgaagctgt taccgtcacg cagcgtttcg acatcataaa
    23041 taatcggctt cttactatcg ccagggcgaa gaaagtagct gtgaaaagaa tgtaccagcc
    23101 gctcgtcagg gacggtctct tttgcagcat acaaggcctg acccacgacc tggccgccaa
    23161 atacctggcg taaacctaaa tcttcactct ggccgcgaaa gagtccttcc tcaatttttt
    23221 ccagatttaa caatgtcagt aaatttttta gcgcctgact catataactc tccagtaaca
    23281 aagctgccgc agcaagccaa agtgagttga gtataacgca aatttgccac tggtccgatg
    23341 ggtgcaatgg tctgaattac gggctaatta caggcagaaa tgcgtgatgt gtgccacact
    23401 tgttgacgtt actattttgt taaccactct tccggcgagg aaagttagcc cgctggtgca
    23461 ttgataataa ggagaagtga atgaaactcg tgcacatggc cagtggttta gcggttgcga
    23521 ttgcgttggc ggcttgcgca gataaaagcg cggatattca gacgccagca ccggctgcaa
    23581 atacgtctat ttcagcaaca caacaacctg ctatccagca accgaatgtc tccggtaccg
    23641 tttggatccg tcagaaagtc gcactgccgc ctgatgccgt gctgaccgtg acactttctg
    23701 acgcgtcgtt agccgatgcg ccgtcgaaag tgctggcgca gaaagcggtg cgtaccgaag
    23761 gtaaacagtc accattcagc tttgttctgc catttaaccc ggcagatgtt cagccgaacg
    23821 cgcgtatttt gttgagtgcg gcgattaccg tgaatgacaa actggtattt attaccgata
    23881 ccgttcagcc ggtgatcaac cagggcggaa ctaaagccga tctgacattg gtgccggtgc
    23941 agcaaaccgc cgtgccggtt caggctagcg gtggcgcaac gactaccgta ccttcaactt
    24001 caccaaccca ggtgaatccg tcttcggcag ttccagctcc aacgcaatat taagcgaagt
    24061 taaccctctc cgcccggctg tctcttatac acaaatcccc acccggggat ttgtgatgtc
    24121 tgtcagtctg ctttaggggg actctttcca tccggtagtt tccttcctcc ccagcagtga
    24181 ttttttgaac tttttcttca cccgttgatc ttctcttata tttcattttc ttcgggtctg
    24241 ttatggtact ttctgattgt tattcatggg ctaacgagca gtttggacat gcccgtctcg
    24301 gtgatccgcg tcgtacacga cgccttgtgt cactggcctc ttctctcgct cagcatgcag
    24361 gtctttctat tgtgaaatca tcccagtcca ctgctcaggt tgaaggtgcg taccgcctga
    24421 tgcgtaaccc gtctatatcc ccggaagcca ttgccgaagc cggatttacc gccactgcaa
    24481 gggcatgtga ggcccatccg ctgctcctcg cgctggaaga caccaccacc attaacttca
    24541 gtcacagtac ggcatttgat gacctgggga ataccaccac taacccaaag acgcgcgggc
    24601 tgctggctca ctctgttctg atgtacgccc ccgacagcgc cctgccagtc ggtcttatcg
    24661 aacagcagcg ctggagtcgt gccacggaca cctacggtgt taagcaccag cgaaaagaac
    24721 gtccctatga agaaaaagaa agttaccgct ggcagcaggc ttcggagcgc atggccgaac
    24781 ggctggggga gatacagaaa cgggtaatca ctgtctgtga ccgggaggca gatatctggc
    24841 attacctgca ttacaaagtc agccacgggc agcgcttcgt ggtgcgtgcc gcacagaatc
    24901 gccggctgga agaggcaccc ggcaagctct tcgaactgcc ggaagtcctg gcaaccgcgg
    24961 gaagccacac gctgaatgtg atgcaaaaag gcggacgggc tgcccgccag gcccggatgt
    25021 tcatcagtta cagcgaagtc agcataaaaa atcccgacaa cagcggccag gcgctcccgc
    25081 tcacgtatgt ctgttgccgg gagcaggcag aggacggtgc ctgctggcat ctgctgacgt
    25141 cagaaaaagt ggcgtgtgcc gccgatgcgc ggcgtatcgt cagccactac gaacgacgct
    25201 ggctgataga ggaatatcat aaggcgtgga aaagtggtgg tacatgtgtg gaatcgctgc
    25261 gaatgcagac ccgggataac ctcgagcgca tgatggttat caaggcgttt atcgcggtgc
    25321 gggtgctggg cctgcggcag ggcggtatca gtgaagaaac acaaaatgac agctgtgaga
    25381 aaatactgac gccgacagag tggaaactgc tgtgggttaa gctggaagga aaaccgcttc
    25441 cgtcacagat gccgacactg aaatgggcct gtctgaaact ggcgaaactg gggcgatggc
    25501 atgacagcaa acgcacaggt cgccccggtt gggtagtcat gtgggatggc tggttcagac
    25561 ttcaggatat ggttgagggt tacctggtga tgaagtctct tgatcaggag atctgatcaa
    25621 gagacagccg cccggagagg gttagtagtt ccagcgataa agctgcaagt cgatttgccc
    25681 gcttcccgac accatcacac cttctgccag taatgcctgt cgctgacgct gtaaatccgg
    25741 tccggtcagt gaaatggtgc cgtggcgatt aaccacccgg tgccagggca aggtgctgcc
    25801 ttcggggaga cgctttaaca caccgccaac ctggcgcgcg gcacggggcg atcccgccag
    25861 tttcgccaca tcaccgtaag tggtgacata gccttcggga atagcggcta cgatttgcca
    25921 gacgcgttgg ggaaatgaat cttctttttc catcttttct tcctgaggta atttttcagc
    25981 ataatctgga aaaacgcccg agtgaagccg cattgcgcaa gaaaccagca tctggcacgc
    26041 aatgggttgc aattagccgg ggcagcagtg ataatgcgcc tgcgcgttgg ttctcaacgc
    26101 tctcaatggg ggctctgttg gttctcccgc aacgctactc tgtttaccag gtcaggtccg
    26161 gaaggaagca gccaaggcag atgacgcgtg tgccgggatg tagctggcag ggcccccacc
    26221 catttctgcc gcccaccgtt tcgtcaaaaa atcccaacat ggctaaactt taaccacaac
    26281 tgacgtcgca agaattgtct ggctgcgcag tacgcttcgg aggtatgtct gatgaagtat
    26341 gttgatggtt ttgttgttgc cgttcctgcc gataaaaagg atgcctatcg ggaaatggcc
    26401 gctaaggccg cgccattatt taaagagttc ggtgcgcttc gtattgtcga atgctgggcc
    26461 agcgatgtac cggatggtaa agtgaccgat tttcgtatgg cggtgaaagc ggaagagaat
    26521 gaagaggttg tctttagctg gattgaatac ccttcaaaag aggtccgcga cgctgctaat
    26581 caaaagatga tgtcggaccc acggatgaaa gagttcggcg agtccatgcc gtttgacggc
    26641 aagcgaatga tctatggtgg attcgaatca atcatcgacg agtaggtagc gtgacgggct
    26701 gtgcggcgtg ccgtcagccc taaaattcac aaatgttgct cggcccagcg taagaaatcc
    26761 tctttcggta atgccttgct gtagagccag ccctgaccgt agtgcacgcc atgctggcgt
    26821 agccactctt cttgtttact ggtttcgatt ccctccgcaa ccattttcag tttcagtgtt
    26881 tttgccattt cgatgatatg cggcgtgaca tttttatatt ccagcgcatc aacgaaagat
    26941 ttatcgatct tcagaatgtc gacatccaga tcctgtaaat agcttaaact tgaataccct
    27001 gtaccaaaat catcgagata aatttcatgg cccgcctccc ggtagcgaga aattatcggg
    27061 gcactggttt tcggatcggc aaactcgcgt tcagtgagtt caagcgcgat ctgtctggga
    27121 ttaacctgat agtgattgat catttcacgc agcaattgcg ggattttttc cgaggtgagc
    27181 acggtggatt caagattgat cgaaatatgc tgctgaggat gctgacgcag ccagtcgccc
    27241 atatcttcaa agacgcttct tataatcagt agcgtcaatg gctcagaaag gcccgtttgc
    27301 tgtgccagcg gaataaaact atctggtgac aaccaactac cgtctgtctg cggccagcgc
    27361 gccagtgcct cagcaccgac aattttgcca ttggctaagg agacaatcgg ctgatagtgc
    27421 acgcaaatat cacgattttc gatagcatcc tgcagccgat gatgcggtga ctggatacgg
    27481 cgcaaaatac gcagcacaaa catcgctgcc agcaggccaa tcaccaatcc gagcggtaac
    27541 caaataaaga cttgccgatg ccagcctttc tgcaacattt tcgttgaagc ccaggtgatg
    27601 atcgaaatat tcatctccgg taagggcagg atatcgtaaa tgattccatt attttcgata
    27661 tgctcaccgg gtgttttttg tagcctggta ataattccct gagcaagttc atcgctgctg
    27721 gttatgacaa cgttatgggc attgccaata atggcggcat caatttgcca tgagctatag
    27781 ggaatgacat caataaagga agcagggtcg atcatgacga cataatgtgc cgttcccatg
    27841 gcgaccatgt aacggataat gcctaaatcg ttatgcgatg ttaaccagac acgataacca
    27901 tctttcgaaa ttttacctgg ctcggggaag gtatcgggcg ggctttcatg ctccagagac
    27961 gaacactggg gaacgttgtt atcgatataa accacttcct gaatatagcg ataactgtaa
    28021 gatacccgac gcatttccat gagatgggct tcgctacagg cagcgccttg ccatctttcc
    28081 agctcttgca gcgcatcttt cccttgtgtc gccaccttat tggcccgaat agcgacgcgg
    28141 gaggaatacg tattcagctc ttcaataaat gatgtttcta cctgctgatg ggccagccag
    28201 atgcttaagc cgacaggcag caatactgaa agaatcagta ctcccgaaat aaggctgacc
    28261 agatgtcgtg ttctcaccag catatcctta ttaacacagt gtagggaaag tatatctgat
    28321 tgtggaaaaa ggctggcagg agataaggag gtagagctgg tatttcatga agtgatgggt
    28381 ggaagcgatc gttgtcattt gaccaggcgt agagctttac gcctggtatg tacccctaac
    28441 ttaagatgtt gattcggcgc gagcgagggt aaaaatatcg taaaaagata gttcaccttt
    28501 acggacgatc attttttgca gttgttcttt atgaacatcc gcgacacgcg gagagttcag
    28561 gatggcttca accagcgcgg caggtacaaa gcgcgtgttg taccattcgc cgttatagca
    28621 aacgcggaaa tccagcacgt cgatatcttc atagcggtag cgtgggtaga catcgaaaaa
    28681 gaagaaacca ttaagcagga taaataatac aaccagcagc cagacagagc ctgaaagcgt
    28741 ttcggattgc aacatcaccg ccagggtagc aaaaaaagca acgtacatac cgataaacag
    28801 ccccggatgt ttacggataa aactgatact aaagcggggt ttgttgtcgc gtttttcgcg
    28861 ggtattcaga gactcaatcg tttctgtcag caggcgttgt atttcggtca tcgtttgcct
    28921 tcgtgatatc tgcaactttc cagggaacac cctattttaa cgtgggaata gtagagggga
    28981 agtaacagat ttgccacgat tttgcataac agttgagaaa agctggttac aattttttaa
    29041 tgattctggc tggattaccg cccacgacaa cgttgtccgg gacatctttt gtgacaactg
    29101 cacctgaggc taccacgacg ttatcaccaa tggtcacacc agggttaatg accgcacgtc
    29161 cgccaatcca gacgttatta ccgatggtga cgggtttccc cagttcagca ccgctattac
    29221 gtgctacagg gtcgatggga tgtgttgccg tgtagatatg aacgcctggt gccaacatac
    29281 agttatcacc aatgcgaata gggcagacat caagcatcac gcaatcgaag ttggcgaaaa
    29341 aattattgcc gagaaaaatg ttataaccat agtcacagcg aaacgttggc tcaatataag
    29401 cctctgtcac ctgaccgaat aaatcagcaa gaatttgctg gcgtaatgtg tgctcttccg
    29461 ccagggaatg attgtatcgg tgaataagac gacgagcgcg caggcgatcg cgagataacg
    29521 cctcatctgc cgagtgatac aactcaccag caatcatctt ttctttttct gtgctcatta
    29581 ctgacctccg gttgctgaaa atgcggcaac acagtaagac gaaaagtcag tgaagagaac
    29641 gttcccatta catctgtgtg atgaagatca catagggtat gtcttcatgg cgaaaaaaag
    29701 cataaaattc ttaataaaca gccggttata gctccgaaag cgagttattt attagcgaat
    29761 aaatttccat actgaggaag ggatcttgtc gtacagttta ttcatggtca attcggcgag
    29821 gcggtgatct gcagctgagt aaaataccgc cagttcatta tctgataatt cgtatttatt
    29881 tttctcgata acacgctcca gcgtgtcaat tgtctgacaa cgacgtaaac gcattaaata
    29941 atcggttttc gttaaaggtt tttcggacat acttctaccc atggttgtaa taattctaac
    30001 aagataaact cgcaggattc tctttcgtcg cattgacaaa acaacggaat agtcgattac
    30061 ctgacttccg ccatttctga agatcctgca tattaatacc ataactactg aacaacataa
    30121 aggtgtcatc cagatattcg tcgatctgct caatgagctt attgtcttca ttatacttaa
    30181 ttttgtaatt aagtgcgaag gtcgcaatat gctcaatcag ttcattcaac tggaggttga
    30241 tcgccgaggt tgggtcgtta acccagccat gattgctttc ttcaaggttt gcaaggcagt
    30301 catgatacag ggtttcacag agaaacttaa gctgtgcgat atcatgtctt ttgggtgagt
    30361 attcatccat aacgcgtccc cttcttagcg gttgaactaa cggacacctt tcgggatgga
    30421 aaaaacttac tgacctggac ttgccctctc gtttgttagc ttaactataa gccactcttt
    30481 gtaggttttc atcgcattat tacgaaaaat tacaattagg aaggaagcgg gacaggactc
    30541 actttttcat gctagcacca acccgcagca ggtaaaagca gtttgctcga actgttcata
    30601 acatttcaga attatcgcca gaagaaattg gtgacactaa taccaggatt gctctgaata
    30661 tgacgtaata accgaggaat gaataaagaa ttaccgcaaa taattaagaa tagcctct
//
LOCUS       DAZFJL010000047        30638 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFJL010000047 DAZFJL010000000
VERSION     DAZFJL010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.047"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(37..507)
                     /gene="ppdA"
                     /locus_tag="ACHJ8C_002060"
     CDS             complement(37..507)
                     /gene="ppdA"
                     /locus_tag="ACHJ8C_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO5551918.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISLIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(692..1486)
                     /gene="thyA"
                     /locus_tag="ACHJ8C_002061"
     CDS             complement(692..1486)
                     /gene="thyA"
                     /locus_tag="ACHJ8C_002061"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO5551919.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(1493..2368)
                     /gene="lgt"
                     /locus_tag="ACHJ8C_002062"
     CDS             complement(1493..2368)
                     /gene="lgt"
                     /locus_tag="ACHJ8C_002062"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO5551920.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(2519..4765)
                     /gene="ptsP"
                     /locus_tag="ACHJ8C_002063"
     CDS             complement(2519..4765)
                     /gene="ptsP"
                     /locus_tag="ACHJ8C_002063"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO5551921.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(4778..5308)
                     /gene="rppH"
                     /locus_tag="ACHJ8C_002064"
     CDS             complement(4778..5308)
                     /gene="rppH"
                     /locus_tag="ACHJ8C_002064"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO5551922.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(5661..5807)
                     /gene="ygdT"
                     /locus_tag="ACHJ8C_002065"
     CDS             complement(5661..5807)
                     /gene="ygdT"
                     /locus_tag="ACHJ8C_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO5551923.1"
                     /translation="MLSTESWDNCEKPSLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            5993..6682
                     /gene="mutH"
                     /locus_tag="ACHJ8C_002066"
     CDS             5993..6682
                     /gene="mutH"
                     /locus_tag="ACHJ8C_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO5551924.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAKRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQIERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            6751..7464
                     /gene="ygdQ"
                     /locus_tag="ACHJ8C_002067"
     CDS             6751..7464
                     /gene="ygdQ"
                     /locus_tag="ACHJ8C_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO5551925.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            7602..7820
                     /gene="ygdR"
                     /locus_tag="ACHJ8C_002068"
     CDS             7602..7820
                     /gene="ygdR"
                     /locus_tag="ACHJ8C_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO5551926.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            7928..8968
                     /gene="tas"
                     /locus_tag="ACHJ8C_002069"
     CDS             7928..8968
                     /gene="tas"
                     /locus_tag="ACHJ8C_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO5551927.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDFAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(9000..10193)
                     /gene="lplT"
                     /locus_tag="ACHJ8C_002070"
     CDS             complement(9000..10193)
                     /gene="lplT"
                     /locus_tag="ACHJ8C_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO5551928.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHILVALAACALAYGGAVVANIYIPKLAAARPGQSWNLISMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(10186..12345)
                     /gene="aas"
                     /locus_tag="ACHJ8C_002071"
     CDS             complement(10186..12345)
                     /gene="aas"
                     /locus_tag="ACHJ8C_002071"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO5551929.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTKALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYARAHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            12931..13962
                     /gene="galR"
                     /locus_tag="ACHJ8C_002072"
     CDS             12931..13962
                     /gene="galR"
                     /locus_tag="ACHJ8C_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO5551930.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETIGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(13969..15231)
                     /gene="lysA"
                     /locus_tag="ACHJ8C_002073"
     CDS             complement(13969..15231)
                     /gene="lysA"
                     /locus_tag="ACHJ8C_002073"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO5551931.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     AGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAAEGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            15353..16288
                     /gene="lysR"
                     /locus_tag="ACHJ8C_002074"
     CDS             15353..16288
                     /gene="lysR"
                     /locus_tag="ACHJ8C_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO5551932.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERIRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFHGENYISLSRIDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(16275..16967)
                     /gene="ygeA"
                     /locus_tag="ACHJ8C_002075"
     CDS             complement(16275..16967)
                     /gene="ygeA"
                     /locus_tag="ACHJ8C_002075"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO5551933.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(17096..18514)
                     /gene="araE"
                     /locus_tag="ACHJ8C_002076"
     CDS             complement(17096..18514)
                     /gene="araE"
                     /locus_tag="ACHJ8C_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO5551934.1"
                     /translation="MVTINTESALTPRPLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(18829..19590)
                     /gene="kduD"
                     /locus_tag="ACHJ8C_002077"
     CDS             complement(18829..19590)
                     /gene="kduD"
                     /locus_tag="ACHJ8C_002077"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO5551935.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(19620..20459)
                     /gene="kduI"
                     /locus_tag="ACHJ8C_002078"
     CDS             complement(19620..20459)
                     /gene="kduI"
                     /locus_tag="ACHJ8C_002078"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO5551936.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDDACVF
                     HMMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVK
                     DLR"
     gene            complement(20746..21927)
                     /gene="yqeF"
                     /locus_tag="ACHJ8C_002079"
     CDS             complement(20746..21927)
                     /gene="yqeF"
                     /locus_tag="ACHJ8C_002079"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO5551937.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVITQSNGQTLVVDTDEQ
                     PRTDTSAEALARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            22182..23411
                     /gene="yqeG"
                     /locus_tag="ACHJ8C_002080"
     CDS             22182..23411
                     /gene="yqeG"
                     /locus_tag="ACHJ8C_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5551938.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            complement(23728..23801)
                     /locus_tag="ACHJ8C_002081"
     tRNA            complement(23728..23801)
                     /locus_tag="ACHJ8C_002081"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23767..23769),aa:Gly,seq:ccc)
     gene            complement(23880..24635)
                     /gene="actS"
                     /locus_tag="ACHJ8C_002082"
     CDS             complement(23880..24635)
                     /gene="actS"
                     /locus_tag="ACHJ8C_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO5551939.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            25049..27346
                     /gene="xdhA"
                     /locus_tag="ACHJ8C_002083"
     CDS             25049..27346
                     /gene="xdhA"
                     /locus_tag="ACHJ8C_002083"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO5551940.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPEIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITSPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFIGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALHSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLIERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            27357..28235
                     /gene="xdhB"
                     /locus_tag="ACHJ8C_002084"
     CDS             27357..28235
                     /gene="xdhB"
                     /locus_tag="ACHJ8C_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO5551941.1"
                     /translation="MFDFASYHRATTLADAITLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELQGITQAEDGALRIGSATTFTQLIEDPVIQRNLPALCAAAASIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLELHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            28232..28711
                     /gene="xdhC"
                     /locus_tag="ACHJ8C_002085"
     CDS             28232..28711
                     /gene="xdhC"
                     /locus_tag="ACHJ8C_002085"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO5551942.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGMPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLFLATWAEGKEIRTLEGEAKGGKLSHVQLAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(28751..30529)
                     /gene="ygeV"
                     /locus_tag="ACHJ8C_002086"
     CDS             complement(28751..30529)
                     /gene="ygeV"
                     /locus_tag="ACHJ8C_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO5551943.1"
                     /translation="MELATTQSVLMQIQPTIQRFAGMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDADNRVQFVNQTALKTLGVVQNNIIGKPVRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANASDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KDVADELGIGIATLYRKIKKYELLNT"
BASE COUNT         7871 a         8041 c         7341 g         7385 t
ORIGIN      
        1 caatcaacac ttccagcaga gaaaaacctt gctcttttac aggcatcctt ctgtttctcc
       61 ttgctgacaa agccggagtc ttccccacgg cgaaaccact agccaccact cgcccgttga
      121 gtttttgaag cgaatatgcc cggcccatgc ggtattgcgc aggccaaaga aagcaagcga
      181 aggtgtcagg tcgctcattt cgacttcggg ccagcgcggc acaaagacca atggtgaact
      241 gccatgacag gtattcgccc cagcaacgga actcacaagg caccataacg tcccctccct
      301 gataagactg atactgtggt cgcggttatg ccagttggca tcttcacgta aatagagcaa
      361 atagtcccgc gcctggctgg cggtttgcca aagccgttgc gactgctgcc agtattgcca
      421 gccataaagc ccacttgcgc ttagcatgac cagaatcagc atcgcgacca gcgtttcaat
      481 cagcgtataa ccacgttgtg ttttcatgcc ggcagtatgg agcgaggaga aaaaaagacg
      541 agggccagtt tctatttctt cggcacatct tccggactat ttacgccgtt gcaggacgtt
      601 gcaaaatttc gggaaggtgt ctcgaagaat ttaacggagg gcaaaaaaaa ccgacgcaca
      661 ctggcgtcgg ctctggcagg atgtttcgta attagatagc caccggcgct ttaatgcccg
      721 gatgtggatc gtatccttca atctcaaagt cttcgaaacg gtagtcgaag atggattcgg
      781 gtttacgttt gataatcaac ttcggcagcg gacgcggttc gcggcttaat tgcagatgag
      841 tttgatccat atggttgctg tagagatgcg tgtcgccacc ggtccagaca aaatcaccca
      901 cttccagatc gcactgctgc gccatcatat gcaccagtaa cgcgtagctg gcaatgttga
      961 acggcaggcc gaggaagacg tcacaggagc gctgataaag ctggcaagag agtttgccgt
     1021 ctgccacata gaactggaag aatgcatggc acggtgccag cgccatttta tccagttcgc
     1081 ctacgttcca cgctgaaaca ataatgcggc gggaatccgg gtcgtttttc agctggttca
     1141 gtacagtagt gatctggtca atatgacgac catccggcgt tggccaggcg cgccactgtt
     1201 taccatacac tggcccgagg tcgccgtttt catcggccca ttcgtcccag atggtgacat
     1261 tgttttcgtg tagataagca atgttagtgt cgccctgcag gaaccacagc agttcgtgga
     1321 tgatggaacg caggtggcaa cgtttagttg tcaccagcgg gaatccatcc tgcaggttaa
     1381 aacgcatctg atgaccaaaa atggaaagcg ttccggttcc ggtacggtcg tttttctgtg
     1441 tgccttcgtc gagcactttt tgcatcagtt ctaaatactg tttcatggtt cctcaggaaa
     1501 cgtgttgctg tgggctgcga cgatatgccc agaccatcat gatcacaccc gcgacaatca
     1561 tcgggatgga aagaatttgc cccatgctga tgtactgcac ccaggcacca gtaaactgcg
     1621 cgtcgggctg gcggaaaaac tcaacaatga tgcgaaacgc gccgtaacca atcaggaaca
     1681 agcctgagac agctcccatt gggcgcggtt tacgaatata caggttgagg ataataaaca
     1741 gcaccacacc ttccagcagc agctcgtaaa gctgtgatgg gtggcgcggc agcacaccgt
     1801 aagtgtcgaa aatggattgc cactgcggat tggtttgcag cagcaaaata tcttctgtac
     1861 gggagccagg gaacagcatg gcaaacggga agttcgggtc aacgcggccc cacaattcac
     1921 cgttaataaa gttgcccagg cgcccggcac caagaccaaa cggaatgagt ggggcaataa
     1981 aatcagagac ctggaagaag gaacgtttag tacggcgggc gaagataatc atcaccacga
     2041 taacgccaat caggcctccg tggaaggaca tgccgccgtc ccatacacgg aaaagataca
     2101 gcggatcggc cataaactgc gggaaattgt agaacagaac ataaccaata cgtccaccga
     2161 ggaagacgcc gaggaagccc gcatagagta agttttcaac ttcatttttg gtccagccac
     2221 tgcccggacg attcgcccgt cgtgttgcca gccacattgc aaaaatgaaa cccaccagat
     2281 acatcaggcc gtaccagtga agcgccacgg gtcctattga gaaaatgacc ggatcaaact
     2341 ccggaaaatg cagatagcta ctggtcatct gtcaccacaa gttcttgtta tttcgctgaa
     2401 agagaacagc gattgaaatg cgcgccgcag gtttcaggcg ctccaaaggt gcgaataata
     2461 gcacaagggg acctggctgg ttgccggata ccgttaaaag atatgtatat gatccgcgct
     2521 ataacccccc gcgaatcagc ccgcccatgc cgcgacgctc cataaaggct gcaacctgat
     2581 ggcgaacttc ggtcgccagt tgcgcttcca gactacgctg cgcaagattt tctgcttcag
     2641 caaaatcaat gcgccgcagc aggtatttta cgcgcgctac agaacgtccg ttcatagaca
     2701 gatggcgata cccaagccca atgaggattg ccacgcacat gggatcgccc gccatttcac
     2761 cgcacaaacg gagatcgatt ccatgtattt ccgcttcccg ggcgatcatc gccagagctc
     2821 gtaacattgc aggatgaaga ctgtcataaa tgttcgccac ccgggtattg ttgcgatcaa
     2881 cggccaggat gtattgagtc agatcgttgg tgccaacaga gatgaaatcg acccgctttg
     2941 ccagatgcgg cagcataaat accattgacg gcacttccag catgatgccg atacgtggtt
     3001 taggaatttc gtaaccgatc atctcctcga cttcacgtcc ggcacgttca atcaggcggc
     3061 gtgcttcgtc aacttcatcg aggcttgtga ccatcggcaa cagaatattc aggttgcccg
     3121 tagcggcatt agcacgcagc atcgcccgca cctggatcaa gaagatctcc ggctgatcga
     3181 gcgtaatgcg aatcccacgc caacccaggc atggattctc ttcgctgatc ggcatgtaag
     3241 gcagctgctt atctgctccg acatccagcg tacgcaaggt gacgggttta tcattaaaca
     3301 tttgcagcat cccctgatac tgcgccacct gttcttcttc cgacggaaaa ccactttgca
     3361 gcatgaatgg gatttcagtg cgataaagtc ctatgccatc aatacggctg cccagttttt
     3421 cttcatgttc cgggcttaaa ccggcattga gcatgacttt aatgcgttcg ccgcttttta
     3481 actgggcggg taaattgacg tcatcttccg ccagacggct aagctcgatc tcttcactaa
     3541 ttagccgctg atattcttgc agcagtaccg gctccggatc gaccagcaat tcaccgcgat
     3601 aaccatcgac gatcagcgtc cgacgatgca gtaccgaagg ctgaatatcc gcgcccatca
     3661 cggtaggtat ccccagcgca cgtaccatga tcgcagcatg ggagttggca gcgccatcgc
     3721 gcacgacaac accgactaag cgatcctggg gcagctcagc aagcgttgtc gctgacagtt
     3781 catctgccac cagaatgaaa cgttccggcc aggcgttcgg cccttgatta gcgtcatcaa
     3841 gatgaaacag caatcgctga cccagcgcac gtaaatcgcc agcccgctct ttgagatagt
     3901 tatcgcttag cgcggcaaac tgttcggcaa atttttcaat gaccgttttt accgcccact
     3961 ctgccaccga gcctttatca acctcggcaa acaattcgcg acgcagccgg gtatccgaaa
     4021 gcaggtgtga gtaaagatcg aaaatagccg ccgtttcttt ttgtgcaccg gcagcaaagc
     4081 gtttgctata gcggcgaaac tcgttagccg cctcttccaa cgccccggtc agtcgttcgc
     4141 gttccagagc cggatccagc gttgatgcct gatacacctg ttccattaaa ggtaacgtgg
     4201 catcctgcca gccttcggca atcgccacac caggtgctgc cggtaatgca cggattcgcg
     4261 tctggcgata ttgcccaaac aaggcagtca actgcgactg agaaagaata gctgccatct
     4321 gggtggcaag cgtcaccagg aaggattctt cactttcgtc atactggcgc aactctcgtt
     4381 gctgtaccac cagtacacca agcaactggc gacgttgaat aattggtacg cctaaaaacg
     4441 cgcggaaacg ttcttctttt acggagggga tgtatttgaa gctggggtgc ttttgcgcat
     4501 ctgcaaggtt tatcggttcc gccagcctgc caaccaggcc gacgatccct tcatcaaacg
     4561 cgagcgttac agtgcgaccg cgtggttttt tcagtccccg ggtcgccatc aggtagtaac
     4621 aacgtcgatc atgatcggcc aggtagaccg aacagacctc ggtatccatc gcaagacaga
     4681 tgtcggtaac cagaatattt aacgcctcat tcaggcgtgg tgcgctggct accttttcga
     4741 ctatttcgcg caggcgagtg agcataattg gcgtgactta acctctttta cgtcgataag
     4801 cagatgcgtt ttgtggtttt ggcgtatttt cctgcagtga catcaccaca ctcgcgaact
     4861 ctttcattac cctacggtag acatcacgtt taaatgacac cacctgtctg actggatacc
     4921 agtaacttac ccatcgccag ccgtcaaact ctggtgtact gctggtttgc atattgattt
     4981 ctgcatcgcc gctcaccagc tgcaagagaa accatttttg tttttggccg atacaaaccg
     5041 gcttcgtgtc ccaacgcacc aaacgtttcg gtaatttgta gcgcaaccag ttacgcgttg
     5101 aagcaaggat tcgaacgtct ttgcggctta atcctacttc ttcaaacaat tcacggtaca
     5161 tcgcctgctc tgcggattct ccggggttga ttccgccttg cggaaattgc caggagtgct
     5221 gaccaaatcg ccgggcccac attacctgcc cctggcgatt acaaatcacg atacctacgt
     5281 ttgggcggta gccatcgtca tcaatcaccg gactacctca aaataaagct ttatatacga
     5341 atgattgttt catactccag gaagacggta aaccactctc tgcagggcat tacacactaa
     5401 taacaattga ataactcaca gttatgtgca gagttataaa cagaggaagg ggtggatagc
     5461 cgtttttatt caccttttct gtggatagag ttgtgaagaa ctacggaatt actacgggaa
     5521 aacccggagc attctgaata agacagatac agagatttta aaatattcat tatcagtcaa
     5581 tgcattacat gtgatttgag taataaaaaa gagtaattcg tgacccaggt cacacctctc
     5641 atttacgggt tggcgaaaga tcaaccaatg ccgtatttat ccacagaatg tgccactaag
     5701 ttaagcactg aaccactaaa aactggagtt tcgtcgcacg tcaaggctgt aaatggaaac
     5761 agtagtgaag gtttttcaca gttatcccag ctttctgtgg ataacatggt gtaagatcct
     5821 gtttattttc agtgaccaga tttggaaaac ccgttgcagt gttgcgcaac tcgattaccg
     5881 gcaacctaaa aagcaatata aatcagagaa ttgaacaacg catgtggaaa aagttacact
     5941 gcgaatattc ggcacttaat tgctgtttgt tttttaacca aggtatcatg acatgtccca
     6001 acctcgccca ctgctctctc ctcccgaaac tgaagaacaa ttgttagcgc aagcacaaca
     6061 actttctggt tatacattgg gagaactggc ggcacttgcc gggctggtta cgccggagaa
     6121 tttaaaacgc gataagggct ggattggcgt gttactggag atctggctag gtgccagcgc
     6181 agggagtaaa cctgagcaag attttgctgc tctgggcgtg gaacttaaaa ctatccctgt
     6241 ggatagtctt ggtcgtccgc tggaaacaac attcgtttgt gttgccccgt taacgggcaa
     6301 tagcggggtg acctgggaaa ccagccacgt gcgccacaag ctcaaacgcg tgctgtggat
     6361 accggttgaa ggcgagcgca gcatcccgct ggcaaagcgt cgcgtaggat caccgttgct
     6421 gtggagcccg aatgaagagg aagaccgaca gttacgcgaa gactgggaag aattaatgga
     6481 tatgattgtt ctcggtcaga ttgagcggat caccgctcgt cacggggaat atttacagat
     6541 acgaccgaaa gcagcgaatg cgaaagcgct gaccgaagct attggtgccc ggggcgaacg
     6601 gattctgacg ctgccgcgcg gattttattt gaagaagaat ttcaccagtg cgctactggc
     6661 ccgtcatttt ctgatccagt agccatcgct ttgacctgcc gctttccggg catataatta
     6721 ccgcttcatt tttttggcag ggctttttag atgttatttg catggataac cgatcctaac
     6781 gcctggcttg cgctcggtac actgacgctg ctggagatcg ttcttgggat cgacaatatt
     6841 attttccttt ctctggtggt ggcaaagctt cccacagcac aacgtgctca tgcgcgccgt
     6901 ctggggttgg cgggagccat ggtcatgcgt ctggcgctgc tggcatccat cgcctgggtt
     6961 acgcgcctga cgaatccgct ttttacaata ttcagtcagg aaatttccgc ccgtgatttg
     7021 atcctgcttc tgggtggctt gttccttatc tggaaagcca gcaaggaaat ccacgaatcc
     7081 atcgaaggtg aagaagaagg gctgaaaaca cgcgtttcat cattcctcgg cgctatcgtg
     7141 cagattatgc tgctggatat tatcttcagc ctcgactcgg tgattaccgc tgtgggtctg
     7201 tcagatcacc tgtttattat gatggctgcc gtggtgattg ccgtaggcgt gatgatgttc
     7261 gccgcgcgct cgattggtga ttttgtcgag cgccatcctt cggtaaaaat gctggcgctc
     7321 tctttcctga ttctggtggg ctttaccctg attctggaaa gtttcgacat ccacgtaccg
     7381 aaaggttaca tctacttcgc gatgttcttc tctattgcgg ttgaaagcct caacctgatt
     7441 cgcaacaaaa agaatccgct ctgatactcc gtacgctctc ctgcgggagg gcgtactcac
     7501 tcctgttacc ctcacagttt aagattttcc tgctttcaag atatataacg tcggtttata
     7561 aacagactat tatcataggt gggccaaatg aggataaccg gatgaaaaaa tgggcagtaa
     7621 taatttctgc agtcggactg gcgtttgctg tttccgggtg ttccagtgat tacgtcatgg
     7681 cgaccaaaga tggccgtatg attttgaccg atggaaaacc tgaaattgat gatgataccg
     7741 ggctggtgag ttatcacgat cagcaaggta acgcgatgca aattaaccgt gatgatgttt
     7801 cgcaaattat tgaacgttaa caaataaggt cagcatccgg ctggccttaa gatttttctc
     7861 tttccctttt cccttccctc tgccattttt atattcctta tgtcgtgatt ataaaaagga
     7921 aacggctatg caatatcacc gtatacccca cagttcgctg gaagtcagca cgctggggct
     7981 tggcacgatg acgtttggtg aacagaacag cgaagccgac gcccacgcac aactcgacta
     8041 tgccgtcgct cagggcatta accttatcga cgttgccgaa atgtacccag tacctccgcg
     8101 ccccgaaact caagggctaa ccgaaaccta cgtcggcaac tggctggcga aacatggcag
     8161 ccgcgaaaag ttaattatcg cctccaaagt gagcggaccg tcgcgcaata atgacaaagg
     8221 catccgcccg gatcaggcgc tggatcggaa gaatatccgc gaagcgctgc atgacagcct
     8281 caagcgtctg cagactgatt acctcgatct ttatcaggtg cactggccgc agcgcccaac
     8341 caactgcttc ggcaaactcg gttatagctg gacagatttt gcgcctgcgg tttcgctgct
     8401 ggatacactg gacgcactgg cagagtacca acgcgcggga aaaattcgtt atatcggcgt
     8461 gtcgaacgaa actgcatttg gcgtaatgcg ctacctgcat ctggcggaca aacacgatct
     8521 gccgcgtatt gtcaccattc agaaccccta cagcctgtta aaccgcagtt ttgaagtagg
     8581 tctggcagaa gtcagccagt atgaaggggt cgaactgctg gcctattcgt gcctgggttt
     8641 cggcacactg accgggaaat atctcaatgg tgcaaaaccc gctggcgcac gtaatacgct
     8701 ctttagtcgg ttcacccgct atagcggtga gcaaacgcaa aaagccgtcg cggcgtatgt
     8761 tgatatcgcc agacgtcatg gcctggaccc tgctcagatg gcgctcgcat ttgtacgccg
     8821 tcaaccgttt gttgccagca ctctgctggg cgcaaccacg atggagcagc tgaaaactaa
     8881 cgtcgaaagt ttgcatctgg agttgagcga agacgtatta gctgaaattg aagcggtgca
     8941 tcaggtttat acgtatcctg caccataaga ttaacgccgg ttaaaaccgg cgttaaatat
     9001 taatgacggc gctgccagat ccacagcgcc gttattgcca gcgcaaacag cgcaccgaaa
     9061 ccaatgccaa tgggcacgac cgggatgcct accattaccg ccagcgagta aatgcccagc
     9121 atcaacaaca tggcgctgtt ttcgccaagg ttttgtactg caatcgcatt gcccgccccg
     9181 acgctttttt taccccgctc ctgtagcaac gcattgagcg gaacgacaaa aaagcccccc
     9241 agcacgccaa tcagcatcaa caaagcatag gctggcagca gctcgtgttg cagggagaaa
     9301 atcagtacca ccacgccaat caaaatcccg gctggcatac agcgtgacac ggtttccagc
     9361 gtaactaact tcgctgccgc acctgcacca accacgatac caatcgctac catcgcgttg
     9421 agataggtcg gcgtagcgtt atcggtaata cccagcgcca ccggtaccca cagcaccaac
     9481 aggaaacgca gcgtgacacc cgctccccag aataaactgg tgcccaccag cgaaaaacgc
     9541 gtttcaccat tgcgccatag cgaggtgcag gcattcagga aactgcgggt catgctgatg
     9601 agattccagg actgccccgg acgcgccgcc gccagtttgg gaatgtagat attggcaacg
     9661 accgcgccac cgtaggccag tgcgcatgcg gccagggcaa ccagaatatg ccagtcagcc
     9721 agaacaccac cagctacgga accgagcaaa atcgccgcta tggtagacgc ttccattaaa
     9781 ccgttagctt ttactaactt actacccgtg gttaattcgc cgagaatacc gtatttcgcc
     9841 ggtgaatagg ctgcagcacc aacacccacc aacgtatagc cgagaaacgg attgatacca
     9901 aagcagatac tggctgcgcc cagtagcttc aggccgttgg caaacatcat cacccggcct
     9961 ttggcgaagc tatccgccac ctgcccgaca aacggcgcaa aaagaatgta agcacctaca
    10021 aacaccattt gcaggatggg ctggctccac tccggataga actgcgcttt cagtaacgcc
    10081 agagtggcaa acagtagcgc attatcgcca aacgcagaga gaaactgcgc cacgataacc
    10141 gctttcatcc ccttcgacca caacgaagtg ttagtgtgca ctgactcact catcgtgttg
    10201 ttccgcttcg tctacccagc ttttcaaagt gacaaagtca ggtttgccgc tgccaagtaa
    10261 tggcatctgt ttcagatagc gaatatcgcg cggtacagca agctccggca cgccgtgcgc
    10321 gcgggcatac tgttgcaact tatcgcgcgt cagttcgtta tctgtggtga aaagcaccag
    10381 tgcctcgcct ttgctggcat cgctcttaat cgcagtggca tggactttat ctggcgaaac
    10441 accaagtgcc agttgttcca ccatttccag cgacaccatt tcgcctgcaa ttttggcaaa
    10501 gcgttttgcg cggccctgaa tctgcacaaa gccctgctca tcaaaacgca caatatcgcc
    10561 agtgtcatac cagccgcgct ccatttcgcc gcgaacattc tcggcggtgg gcacttccag
    10621 tacacctggc ttctccaccc gcagatagcc gttcattatg ttcggccctt tcagttgcag
    10681 gcgtccgccc tcttcgatac caggaaccga caacaggcgc gcatccattc ctggtagaat
    10741 acgccctacc gtaccgggtt tcgccgccat cggtacgttg atagaaacga caggcgcgca
    10801 ttcggtcaca ccgtagcctt caaggatgcg caggccaaat ttatcctgcc aaagctgttt
    10861 agtactttct tgtagttttt ctgcgcctgc caccacatag cgtagacgat agaagtcata
    10921 cgggttggca aagcgcgcgt agtgaccgag gaaagtcgag gtaccgaaca acacggtgca
    10981 actgcggtca tacaccaact ccggcacaat gcggtaatgc agcgggcttg gataaaggaa
    11041 cacttctgca cctgtgagca gtggcgtaaa caggcctacc gtcagcccaa aggagtgaaa
    11101 cagcggtaac gccgacataa agcgatcgtt ggtggtgaag tcggcaatcg ttttaatctg
    11161 ctcgacgttc gccagaatgc ttttatggct atggacgacc cctttcggat ggccttcaga
    11221 accggaggta aagaggatca gcgcctcttc ttccggctgc tgttttacct gtgccagacg
    11281 cggcatcagc aaatgagcga aaatccacac tttgtcggca gtggtgacat ctgcttttaa
    11341 atcttccaga tagacccagc gcacctgagt aagttgctcc ggcagatgcc agagtttgcc
    11401 tttatcgaga aactgacggg aagtgaagat ggttttgatt tcagccgccg taatagcact
    11461 ggtcaaccct tttaccccgg cggtgtagtt catcattgcg ggaatgcggc gacgggcgat
    11521 ggccccaaaa atcactgccg cactgatgcc tgcattgggc agcattaagc cgatgcgttc
    11581 gccttcaaca ctgtattttt caaggatgcg tccaacaaac agcgttttcg taagcaattt
    11641 gcgataggag tccggggtaa agttgacgtc ttcgacacat ttcttcccgg ctccgaagcg
    11701 gtacattgcg ctcagtaaag attcgtacag cgtttcacgc gggcgcaccg ccatacgcgc
    11761 ctccatcatt atttgatgca gcatttcgcc agcgattttg cgacggtcac gggcacgcgg
    11821 cgcatccggc atctccacct gcgttggtgg caaaatatgc agagtaattt gcgggaacaa
    11881 gcgacgttta accagacctt tcaggcggct gaagtgcgta agttccgccc cttcaatacg
    11941 cacaggaata accgttgcac cagacttcgc cgcgacaaat cccgcgccat cgtagatttt
    12001 catcagcgag ccagtggtgg tgatgcgtcc ttcagggaaa atcaccactg gtcggccctg
    12061 ttccaccaga cgtaccagat gtttaatcgc cataggttgc gtcgggtcga gaggaacaaa
    12121 gtcgataaat gatttcagcc aacgcatata ccactgttgg cttattgagg tgtaaacggc
    12181 aaataccgga cgtacgggta aaaacaatgc cagcagtatg ccatcaataa aagagacgtg
    12241 attaggcgta attagaacgc gctcgccctt cagtgccttg gtgtcacccg taacgcgaac
    12301 gcgatacaaa acacggcaca aatttcgaaa aaagctaaaa agcattccaa ctccctttgc
    12361 tctgattcag taaaagcgaa tggagggaga ttacacgaga taaagaacgc gagcgacagt
    12421 aaattaggtg cgaaaaaaaa cctgcgcatc cgcgcaggtt ggtgcaagag acagggtacg
    12481 aagagcgtac cgaataatct caccaatcaa tacctctggg atcttgattg tggtctgcac
    12541 gacgactctt cgccagcgag aaaacgcaaa ggaatgaagg gaaatgcaac gaggtgtgta
    12601 aattgtcggt tactgttaca gattgatgac cggcaaaaaa aaacctgcgc atctgcgcag
    12661 gctggtgtaa ttcatgtgct caacccgaag ttgacttcac ctatcaatac ctctgggatc
    12721 accactttag caacctgaag ccaaacgcca ccagcggtca atcgcaacag cgtttcgcaa
    12781 agtgtaacca aaggtttgca atctcttttt ttgtcttact tatttgcgtt tggctcacat
    12841 tcccacaatg aaaacacgcc gccccttgaa ccaacggccg ttttccgtaa cactgaaaga
    12901 atgtaagcgt ttacccacta aggtattttc atggcgacca taaaggatgt agcccgactg
    12961 gcaggcgttt cagtcgccac cgtttcccgc gtcattaata attcacccaa agccagcgaa
    13021 gcttcccggc ttgctgtgca tagtgcaatg gagtctctta gctatcaccc gaacgccaac
    13081 gcccgtgcgc tggcgcagca gaccactgaa acgatcggtc tggtcgttgg tgatgtttcc
    13141 gatccgtttt tcggtgcaat ggtgaaagcg gtcgaacagg tggcttatca cactggtaat
    13201 tttttattga ttggcaacgg ttaccacaac gaacaaaaag agcgtcaggc cattgagcaa
    13261 ctgatccgcc atcgctgtgc tgcgctggtt gtccatgcca aaatgatccc ggatgccgat
    13321 ttagcctcgt taatgaaaca aatgcccggt atggtgctga tcaaccgtat cctgcctggc
    13381 tttgaaaacc gttgtattgc tctggacgat cgttacggtg cctggctggc aacgcgtcat
    13441 ttaattcagc aaggtcatac ccgcattggt tatctgtgct ctaaccactc tatttctgac
    13501 gccgaagatc gtctgcaagg gtattacgat gcccttgctg aaagtggtat tccggccaat
    13561 gaccggctgg tgacatttgg cgaaccagac gaaagcggcg gcgaacaggc aatgaccgag
    13621 cttttgggac gaggcagaaa tttcactgcg gtagcctgtt ataacgattc aatggcggcg
    13681 ggcgcgatgg gcgtgctcaa tgataatggt attgatgtac cgggtgagat ttcgttaatt
    13741 ggctttgatg atgtgctggt gtcacgctat gtgcgtccgc gcctgaccac cgtgcgttac
    13801 ccaatcgtga cgatggcgac gcaggctgcc gaactggctt tggcgctggc ggataatcgc
    13861 cctctcccgg aaatcactaa tgtctttagt ccgacgctgg tacgtcgcca ttcagtgtca
    13921 actccgtcgc tggaggcaag tcatcatgca accagcgact aaccgcagtt aaagcaattc
    13981 cagcgccagt aattcttcaa tggtctggcg acggcgaatc aaccgcgcct gaccattatc
    14041 aaacagaact tctggtaaca gcggacggct gttgtagttg gatgacattg atgcgccata
    14101 tgcccctgta tcatgcagta ccagataatc acccgccttc acttccggca aggcgcgggt
    14161 ttcgacattt cccccttcct gctgggtaaa gacatcgccc gattcacata acggcccggc
    14221 gacgacggtt tccaccgttg gcgcgtgttc cagagaacga ccctcagctg ccagggcact
    14281 gatatggtgg taactaccgt acattgccgg gcgcatcaga tcgttgaacc cggcatcaac
    14341 cagcacaaag tggcggctac ccatttgttt gacgctccgc acttgagtaa ttaatacgcc
    14401 agcctgcgct accagaaagc gacccggttc aatttccagt ttcacaggat ggcccaaatg
    14461 gcgggcgatt tgctcacgcg cggcattcca cagaccataa taatgttcgg tatcaaccgc
    14521 ctcttcaccc tgttgataag gaatagaaag cccaccgccc gcagaaatag cctgtaaatc
    14581 ctgaccgaac tcgaggacct gacgcaccat agcaccacat acctgttcca gatgggcata
    14641 atcgacgcca gaaccaatgt gcatgtgaat gccgaccagc tgtagatgat gacgttgtat
    14701 cacgtccagt gcggcgggca gatcggtgta ccagataccg tgcttgctgt tttcgccacc
    14761 ggtattggtt ttttggctat gcccgtgacc aaaccccgga ttaacacgca gccatacccg
    14821 atgccctgga gaaacctgac ccagttggtc gagcatatca acagaacccg cattcaccgg
    14881 aatttgcaat tcactgacgc gttcaagcgt cgcctgatcg ataacatctg ccgtaaaaac
    14941 aatatcatcg gggtgcgttt gcggattgta acccgccgcc agtgcacgct ctatttcgcc
    15001 taacgagacg gaatccactt ttacgccctg ctcacgcatt aagcgcaaaa tatgaatatt
    15061 ggaacaggct ttctgtgcaa agcgcaccac atcaaactgt ttcagcgctg caatctgccg
    15121 acgaataatt tgcgcatcgt agacccacac cgggcagcca aattctgcgg gcaaacgcag
    15181 cagattttcg gcggtgagat cggtatcagt gctgaacagt gaatgtggca taacaaactc
    15241 cagataagtg cttttttatg attacgccac atgataaaaa gaataaaaaa tatcgattta
    15301 tgtcgagtct atgcaaaaat gatatggatc accggaatgc gagagagcgc taatggccgc
    15361 cgttaactta cgtcatattg aaatttttca tgcggtaatg accgccggaa gcctgactga
    15421 ggcggcacac ctgctacaca cctcacagcc aaccgtcagc cgcgaactgg cgcgctttga
    15481 gaaggtgatc gggctgaaat tgtttgagcg catacgtgga cgattacatc ctaccgtgca
    15541 aggactgcgt ctgtttgaag aagtgcaacg atcctggtac ggactggatc gcattgtcag
    15601 tgccgcagaa agtctgcgcg agtttcgcca gggagaactg tctattgcct gcctgccggt
    15661 cttttcgcaa tcttttttac cgcagctcct gcaacccttt ctggcacgtt atcccgatgt
    15721 cagcttaaac atcgtgcccc aggaatcacc gctacttgaa gagtggctct cggcccagcg
    15781 tcatgattta ggactcactg aaacgctcca tacgcctgcg ggaacagaac gtaccgaatt
    15841 actctcttta gatgaagtgt gtgtgttacc tcctggccat ccgctggcgg taaaaaaggt
    15901 attaacgccg gatgattttc acggtgaaaa ctacatcagc ctttcccgta ttgacagcta
    15961 tcgccagttg ctggatcaat tgtttactga gaatcaggtt aaacgacgca tgatcgtaga
    16021 aacccacagc gccgcgtcag tctgcgcaat ggtacgggcg ggggtaggcg tttcggtagt
    16081 taacccactc accgcactgg attatgcggc aagcggttta gtggtgcggc ggttcagcat
    16141 tgcggttccg ttcactgtca gcctgatccg ccccctgcac cgcccgtcat cagcgctggt
    16201 gcaggcgttt agtgagcatt tacaagcggg attaccgaaa ctggtcactt ctcttgacgc
    16261 tattttgtcg tcagctacga cagcataaaa gcgacagcat cctcggcatg gatcgccgcg
    16321 gtatcaaaca caggcagaac actgcgctct tctggcacca gtaaaccaat ttctgtgcag
    16381 ccaaaaatga cgccctgtgc gccctgttct gcaaggcgag caatcacttg cgcataataa
    16441 gcgcgtgacg cttcggtaaa ttgccccaga cacagttctt caaaaataat ctgattaatt
    16501 tttgcccgct catccgcttc aggaataaga cagttgatgg aaaattgttc cgtcagccgc
    16561 ccgcgataaa aatcctgttc catggtgtaa cgtgtaccca gcagcgccac acgagtcatt
    16621 cctgccccgg taattgcacg tccagtggca tccgctatgt gtaagaaagg cagagagcaa
    16681 cgagactcaa tggcatccgc cactttgtgc atcgtattgg tgcataacac aataccttct
    16741 gcgcccgccc gctgtaagcc aagcgccgcc tcagccagaa tatccccggt tttgtcccac
    16801 tcaccgcgac gctggcactc ttctatttca tgaaaatcga cgctatgtag cagcacttgc
    16861 gcagagtgaa gcccaccaag ccgctgttta atgccttcat ttatcagacg atagtaagga
    16921 atagtggatt cccagctcat tcctcccagc aaaccaattg ttttcattcc tctctcctga
    16981 tgtgtgttac cgcaacaagg ctgaataagg aacgagacaa acgcctcaac ggccaggtgc
    17041 ccaatctcta ttaacgaaaa aagggccgga tgtacagcac atccggcccg tgaaatcaga
    17101 cgccgatatt tctcaacttt tcgcctgcca tcagtttgcg ctcgatatgt tccagcgtga
    17161 catttttggt ttccggaatg agccagaaag tgatgcccac aaacgcaatg ttcagcgcag
    17221 tgtagagcca gaacgtaccg gcagcgccaa tgctatcaag cagtgtcagg aaggtcgcgc
    17281 cgataatcat attcgacacc cagtttgtcg tcgttgagca ggtgatgcca aaatcgcggc
    17341 atttcagcgg ctgaatttca gagcacaaga tccacaccac tggcgcggcg ctcatcgcat
    17401 aaccggcaat acacatcatc gtcatgccaa cagagagcca ggacaagcca ctggaagccg
    17461 taccgttatc aaactgcatc aggcagtaac ccagcaccag agtgcctaac gccatcacgc
    17521 tgaaaccaat tttcagagcc ggcttacgcc ctgctttatc taccgtaaag accgcaatga
    17581 aggtagcgaa cataaaggtc agccccacga ccagagtcgc aatcatctgt tgttctgtgg
    17641 tcgtaaagcc cgccattttg aagatacgtg gcgcgtagta catgatgatg ttcataccgg
    17701 taaactgctg catcgcctgc aacaacatac cgagaaatac agcacgacgg acgttacggt
    17761 tgatcttaaa cagtgcccaa ccgccctgtt ttaacttcag gctttcacga atttcgttga
    17821 gttcttctcg cgctttttcc gacgtatcgc gcagcatacg caatacttct tccgcctcaa
    17881 tatgacgccc cttttccgcc agccagcgcg ggctatttgg caggaagacc accagaataa
    17941 tcagcagaac tgctggtaaa gcaagaaccc ccaacattgc gcgccagtta ccgctataac
    18001 tgaacgctgt atcggataaa aacgccagca cgatgccgag agtgaccatc aactggtaca
    18061 tgctgatcat cttaccgcga acgttttcac ttgccatttc agaaagatat agaggagcgg
    18121 tgtaagacgc gatcccgaca gcaatgccca acaccacacg agcggcgatt aacatctcta
    18181 cgctggtcgc aaaagcggac cctatcgaac cgagtacaaa caggatggcc ccagccatca
    18241 ggctgtattt acgccccagg cggaacgaca gccaaccatt aaacagcgca ccaattgctg
    18301 cgccgagcat catgctactg accacccatt cctgcaaacg actggtcagc acaaagtgat
    18361 cggtaatgaa cggcaacgct ccggcgatta cgccgatatc aagaccaaat aacaatcctg
    18421 cgactgcagc agctaccgaa acaaacatat tcatacgccg cgtatcccgc agaggacgtg
    18481 gcgttaaagc agattccgta ttgatagtga ccattttttc ctgccagcag agagtaagac
    18541 atagtgaaaa aatacgtgaa caattcacac aggtgtcagg tcggaaatag cataaatatg
    18601 gattaaattg ctacgacatg tcgttatgtg atggatattc caattttcaa attaagttga
    18661 attattgaga ttattattaa ctacctaatt ttaaagcaga taaaattcat aaagttcatc
    18721 aattgataat tgatatggat tatttcataa ccatgatatg gattatgatg atctacaggt
    18781 ataaaaaacc ctgccatgcg acagggtcat aaaagtaaga ggaatgaatt aacgcgccag
    18841 ccaaccgcca tccacggcaa tggtataacc attcacataa tctgaagcgc tggaggcaag
    18901 gaacaccacc ggccccatca ggtcactcgg cagtccccaa cggccagctg gaatgcggtc
    18961 gagaatttcc gcgctacgtt gttcatctgc ccgcagttgt tgagtattgt tggtcgccat
    19021 gtaacccgga gctatcgcat taacattaat gttgtgttta gcccattcgt tcgccatcaa
    19081 tcgcgtcacg cccatcacac cgctttttga tgcggtataa gaaggcacac ggatcccgcc
    19141 ctggaaggag agcattgacg cgatattgat aatcttgccg ccattgcctt gcgcgataaa
    19201 gtgtttcgcc gctgcctgag acatgaagaa tacgctcttg atattcaggt tcatgacatc
    19261 gtcccagtct ttttcgctga attcgagagc gtcttcgcgg cgaatcaatc cggcgttatt
    19321 caccaggata tcaatatgac caaactcagc taccgcgcga tccagcagtg ctggaatgcc
    19381 atcaatcttt cgcagatcgg cggtcaggct taaaaaacga cgccccagcg ccgtgacctg
    19441 cttgatggtt tcagtcggtt caacgatgtt aatgccaaca atgtcacagc ccgcttgcgc
    19501 cagccccaac gccatcccct ggcccagccc agtatcacaa ccagtgacga ccgcaacttt
    19561 accttcgaga gaaaatgcac ttaaaatcat aacaatacct tattcgttta tgcccacaac
    19621 tagcgcaaat ctttaacggc cacatggtcc atatcatcaa aaacctggtt ttcaccgacc
    19681 atgccccaga taaaggtata agctttggtt ccgacgccgg aatggatcga ccagctcggg
    19741 gagatcaccg cctgctcgtt atgcatcaca atgtgacgcg tttcttgcgg ttgccccatc
    19801 atgtggaaaa cgcaggcgtc atcatcatcc atattgaaat agaaataaac ttccatccgg
    19861 cgctcgtggg tgtgacacgg catggtgttc cacaagttac ctggagccag ctccgtcagc
    19921 cccatactca attggcaggt ttccagcaca tccgggacaa aatatttgtt aatcgtgcga
    19981 cggttactgg tgaggttatc gcctaacgtg actggagata cttcgtccgg tgtgactttt
    20041 ttggtgggat acgtcgtatg cgcgggtgcg caattgtaat aaaacttcgc cggagtggcg
    20101 gtatcaatac tggcaaagac aacttctttt gcacctttac caacatacag ggcgtcgcgg
    20161 tgaccgattt catagcattg accatcgaca gtaatcgtac cggcaccgcc aatattgata
    20221 acacctaact cgcgacgttc aaggaaatag cttacgccga gttgtttacc aacttccccg
    20281 ccaacggaaa ccgttttagt taccggcata atgccgccaa caataatacg gtcaatgtgg
    20341 ctgtaaacca tggtgtactc atcggcgaca aatacctttt caaccaaaaa ttcattgcgc
    20401 agcccctggg tatccagcgt ttttgcgtgc gcactgtgga tgctctgtct tacgtccaca
    20461 tcaacctccg ataatcagtg aagcgtgaaa atagaaaaca acaatgaaac actgtttcgt
    20521 tttctctatg aggggatgat atgcccatgg gatagggttt tcaattaaaa ataaaacaat
    20581 gtttcatttt caagttaagg attaaaaaaa gtgcagtgtc gatcacgaat atgtcatgag
    20641 aatggaaaaa tcagggagcc ctgaaaaggt atgtcgactc accagccatc attattcaac
    20701 agattaatga cggcagatat ttatcggtaa ggaggaaatg cagagctact cgtcacgttc
    20761 aatggtcaat gccacaccct ggcctccgcc gatacaaagc gttgccagtc ctttgcgggc
    20821 attacgtttc accatttcat gaacaagaga aaccaggatt cggcaaccgg aagcgcctat
    20881 cgggtgaccg agtgcgatcg caccaccatt aacattgacc cgacgctcat cccattcaag
    20941 catcttgcca accgaaagcg cctgtgcagc aaacgcttca ttagcctcga taagatcgac
    21001 atcagccaac tgccagccaa cacgctccag gcaacggcgg gtcgcataca ccggcgcaat
    21061 tcccatcaat gccggatcca caccaacgct ggcaaatgcg cggatgcggg ccagcacggg
    21121 taaattcaac gctcgtgctt tggcttcgct catcatcatt actgcagctg ccccatcgtt
    21181 tatggatgat gcattacccg ctgtcacaga accgagacta tcaaatgaag gatttaaacg
    21241 ggctaaggct tctgcgctgg tgtcagtgcg tggctgttca tcggtatcaa caaccaacgt
    21301 ctgaccgtta ctttgggtta ttaccgggac gatctcatct ttaaatcgtc cggcgtcaat
    21361 cgccgctcgc gctttttgtt gcgagctaag tgcgtaagca tcctgcaact gacggctgat
    21421 gccatattcg cgagccagat tttcggcggt gacaccaata tgataatcat tgaaggcatc
    21481 ccacaaccca tcatgcacaa gactatcaac caactggcta ttgccaagct gtgcgccagt
    21541 gcggctatca gtcagaacat gtggtgcgcg gctcatgttt tcctggccac cggcgatgac
    21601 aatatcagcc tcgccacact gtatcgcctg agtagccaga tgcagtgctt taagcccgga
    21661 accacaaacg tcattaatag tgattgcaga aacgctatta ggaagaccac ctttaatagc
    21721 cgattgtctt gccggattct gccctgcccc tgcagtcaac acctgaccaa gaatcacttc
    21781 atccaccgca tatgcaggaa cgccggtacg ttctattaac gctttgacga ccaggctgcc
    21841 aagttccacg gcggaatgac ccgctaacgc accacgaaag cagccgatag gtgtccgtaa
    21901 cgccccgaca atcacaacgt ctttcatcac aacctcgcac caattaacag tgcaatagta
    21961 aatgattgtt aattacagtt atcttaattg tttaaaaaaa gtgactatta tcacaaagga
    22021 aatacgcctg agtagtagtc agagacataa ctggcacgta aggtttgcaa ccactaactc
    22081 accaatagag aggtagatag ggcgttaatc tcccatactt aacctggttt atggtaaatt
    22141 gccctccatt ttgtttaatt tgtagatgat acgttcagat aatgtctaat atttggtcaa
    22201 aagaagaaac tctgtggagt ttcgcgctct acggcacagc cgttggtgca ggcacgctct
    22261 tccttcctat tcagttaggt tcggcggggg ctgtggtcct gtttattact gctctggtcg
    22321 cctggccttt aacatattgg ccacataaag ccttatgcca gttcatcctc tcatcgaaaa
    22381 catcagcagg tgaagggata acgggcgcgg taacacacta ctatggcaag aagattggta
    22441 atctgattac cacgctgtac ttcatcgcct tttttgtcgt cgtgttgata tatgcagtgg
    22501 caattaccaa ctcacttacg gaacagctgg caaagcatat ggttattgat cttcgcatcc
    22561 gtatgttggt gagtctgggg gttgtattaa tcctgaatct catttttctg atgggacgcc
    22621 atgcaaccat tcgggtaatg ggatttttgg tattcccatt gattgcctat ttcttatttc
    22681 tttctattta cctggtcggt agttggcaac ctgatctatt aacaacccag gtagagttca
    22741 atcagaatac ccttcaccag atatggatat cgattcccgt gatggttttc gcttttagcc
    22801 atacgcccat tatttctacg tttgccatag acagacgtga aaaatatggc gaacacgcta
    22861 tggataaatg caaaaaaatt atgaaagtcg cttatctcat catctgcata agtgtattgt
    22921 tctttgtctt tagctgcctg ctttctattc caccttcgta tattgaagcg gctaaagaag
    22981 aaggggttac cattttatcg gcgctttcta tgctgccgaa cgccccagca tggttgtcaa
    23041 tttccgggat tattgtcgca gtagttgcga tgtcgaaatc attcctgggt acgtactttg
    23101 gcgttattga aggtgccaca gaggtcgtca aaacaacatt acagcaggtt ggtgtaaaga
    23161 aaagtcgtgc atttaaccgc gcactatcaa ttatgttggt atcgctgatt accttcattg
    23221 tttgttgcat taacccgaac gcgatttcga tgatttacgc gatcagcggc ccgctcattg
    23281 ccatgatact tttcatcatg cctacgctgt caacgtatct catcccggcg cttaaaccct
    23341 ggcgttccat cggaaatctg attacgctga tcgtgggtat cctgtgcgta tcggtaatgt
    23401 tctttagcta atatccgttg tggatggttc aaaattgctt caattttgaa ccatcccagt
    23461 tttcataatg ttcatttgtt attattccgc tctatcactt ctctcgtaat attatataat
    23521 tatcagccag ttaaacacac aaagagttct gaacatctta aataagacta tttaagatac
    23581 atgacgattg cattgcgtaa acgctttttg tttacaaaaa aatggggaag tattacggcg
    23641 attgcgaaaa gtctgaagcg cttgaattaa gataatatag aaaatcagat acaacaaaaa
    23701 tggcaggtag atgatgtgaa gataaattgg agcgggcgaa gggaatcgaa ccctcgtata
    23761 gagcttggga agctctcgtt ctaccattga actacgcccg cttcgagatg cgtaaggcat
    23821 tataaacctt acgctctcct tagcaagtac cacgctgctg actgctgatt aattcgccat
    23881 cagcattttg gcttgctgcc ctgaggcggc aagtaacgta gcggatcaat tgccgttgca
    23941 cggtaacgaa tctggaaatg caggcgaaca gatgccgcat cagtgctccc catagtggcg
    24001 attttttgcc cagctttcac gctttgccca ttatttacca gcatcgtgtc attatgagcg
    24061 taagccgtaa tgtaatcttc actgtgttta atcatgatga gattaccgta gccacgcagc
    24121 tggttaccca catacaccac ctttcctgca cccgcggcgt aaataggtgt acctcgtgga
    24181 gctgagatat caatcccttt attgccgcca tctgctgtcg aatacggcat gataactttc
    24241 cctgtcgttg gccataacca acaacgttgc cctactggcg gccaggaaga tttcggtacc
    24301 gctgatgacg gtgtaaccga tgcggttttg gtcgttgatt tggcggttga tttacgtgta
    24361 ctactgctac ttttcgcccc acccagtttt agtttctgac ctacttcaat ggtgtaaggg
    24421 ggggaaatgc cgttcagtcg cgccagttct tttacgctgg ttcccgtggt gcgcgaaata
    24481 cgatatagcg tatccccccg tttcacggtg taaacggagc cggaatacgt tcctgtatcg
    24541 gatgatttgc tacccgaaca gcccgccaaa agcagtccaa ccgataacaa catcacgata
    24601 cccagagatt ttttattcag gcgtcccgca ctcaaaatct ttcctcgctt aaaaaacaag
    24661 acgccatatc atagcagcca gcctcttgat acccaatctc ttgcttctgg aaaaacgatg
    24721 taatagagaa agaggttaat ttcatttggc aaagggaagt atcatcagca aaatatttgc
    24781 tgtagtggta tcgaagttcc tgacttttac cctatttccc accttgatgc gtaataatcc
    24841 taatttatca cattgatttt atcgccactc atattgatac gtatcactgt ccctattcag
    24901 agtcctgttt cgtatcatat ctgtgttttt ttaacgtcat cacacttcct tcctccccga
    24961 acgcttatcg tcgtttctgg cgtaaatctt gcctgcttag actaaatctt tgccataaga
    25021 accaaatgtt caaatcgagg ggatttctat ggaagcgcgg gaagcaaccg ctacgggtga
    25081 atcatgcatg cgcgtcgatg ccattgctaa ggtcaccggg cgggcacgat atactgacga
    25141 ttatgttatg gcgggcatgt gttacgcgaa atatgtacgt agccctatcg cacatggtta
    25201 tgctgtaagt attaatgatg aacaagccag gagtttgccg ggcgtcctgg cgatttttac
    25261 ctgggaagat gtgccagaaa tcccattcgc cacggcaggg catgcctgga cacttgacga
    25321 aaacaagcgc gataccgccg atcgcgcact gctaactcgc catgttcgtc atcatggcga
    25381 cgccgttgcc atcgtcgtgg cccgcgatga actcacggca gaaaaagcgg cgcaattggt
    25441 cagcattgag tggcaagaat tacccgttat cacctcgcca gaagcggcgc tggcagaaga
    25501 cgctgcacca atccataacg gtggcaattt actgaaacaa agcacgatgt cgacgggtaa
    25561 tgtccaacaa acaatcgatg ccgccgacta ccaggtacag gggcactatc agactcccgt
    25621 tattcaacat tgtcatatgg aaagcgtgac atcgctggca tggatggagg atgactcgcg
    25681 aattaccatc gtttccagca cccagatccc gcacattgtt cgccgcgtgg ttggtcaggc
    25741 gctggatatt ccctggtcat gcgtacgagt catcaaaccg tttatcggtg gcggttttgg
    25801 taataaacag gatgtactgg aagagccaat ggcggcattc ctgaccagca aacttggcgg
    25861 cattccggtg aaagtttccc ttagccgtga agagtgtttc ctcgcaaccc gtacccgcca
    25921 cgcttttact attgacgggc aaatgggcgt gaaccgcgac ggaacattga aaggttatag
    25981 tctggatgtt ctgtctaaca ccggcgctta tgcatctcac gggcactcca ttgcttctgc
    26041 tggggggaat aaagtcgctt acctttatcc tcgttgtgcc tacgcttaca gttcaaagac
    26101 ctgctatacc aacctcccct cggctggtgc gatgcgtggt tatggcgcgc cacaagtcgt
    26161 atttgccgtt gagtctatgc ttgatgatgc cgcgacagcg ttaggtattg atcctgttga
    26221 aattcgttta cgcaacgccg cccgcgaagg agatgctaat ccgctcacgg gaaaacgtat
    26281 ttacagcgca gggttgccgg agtgtcttga aaaaggccgg aaaatctttg aatgggaaaa
    26341 acgccgtgca gagtgccaga accagcaagg caatttacgt cgtggcgttg gcgtcgcctg
    26401 ttttagctac acctctaaca cctggcctgt cggcgtagaa atagcaggcg cgcgcctgtt
    26461 gatgaatcag gatggaacca tcaacgtgca aagcggcgcg acggaaatcg gccagggtgc
    26521 cgacaccgtg ttctcgcaaa tggtggcaga aaccgtggga gttccggtca gcgatgtccg
    26581 cgtaatttcg acacaagaca ccgacgttac accattcgac cccggcgcat ttgcctcacg
    26641 tcagagctat gttgccgcgc ctgcgctgca cagtgcggca ctgttattaa aagagaaaat
    26701 catcgctcac gccgcagtca tgctacatca gtcagcgatg aatctgaccc tgataaaagg
    26761 ccacatcgtg ctgattgaaa gaccggaaga accgttaatg tcgttaaaag atttggcgat
    26821 ggacgctttc taccaccctg aacgcggcgg gcagctctct gccgaaagct ccatcaaaac
    26881 caccactaac ccaccggcgt ttggctgtac ctttgttgat ctgacggtcg atattgcact
    26941 gtgcaaagtc accatcaacc gcatcctcaa cgttcatgat tcgggccata ttcttaatcc
    27001 gctgctggca gaaggtcagg tacacggcgg aatgggaatg ggcattggct gggcgctatt
    27061 tgaagagatg atcatcgatg cgaaaagcgg cgtggtccgt aaccccaatc tgctggatta
    27121 caaaatgccg accatgccgg atctgccaca actggaaagc gcgttcgtcg aaatcaatga
    27181 gccgcaatcc gcatacggac ataagtcact gggtgagccc cccataattc ctgtagccgc
    27241 tgctattcgt aacgcggtga agatggctac cggtgttgca atcaatacac tgccgctaac
    27301 gccaaaacga ttatatgaag aattccatct ggcaggattg atttgaggat aacatcatgt
    27361 ttgattttgc ttcttaccat cgcgcaacca cccttgccga tgccatcacc ctgctggctg
    27421 acaatccgca ggccaaattg cttgccggtg gcactgacgt actgatacag cttcaccatc
    27481 acaatgaccg ctatcgccat attgttgata tccacaatct ggcagagctt cagggaataa
    27541 cacaggcgga agatggcgcg ctgcgaatcg gctctgcgac aacatttact cagctcattg
    27601 aagatcccgt aatccaacgc aatctcccgg cgttatgtgc tgcggctgca tcaatcgccg
    27661 ggccgcagat ccgtaatgtc gccacctacg gcggaaatat ttgcaacggt gccaccagcg
    27721 cagattctgc cacgccaacg ctaatttatg acgcgaaact ggagctccac tccccacgcg
    27781 gtgttcgttt cgtcccgatt aatggctttc acaccgggcc gggcaaggtg tctcttgagc
    27841 atgacgaaat ccttgtcgcc tttcattttc cgccacagcc gaaagaacac gcgggcagcg
    27901 cgcattttaa atatgccatg cgcgacgcaa tggatatttc aacaattggc tgcgccgcac
    27961 attgccgact ggataacggc aatttcagcg aattacgcct ggcatttggt gttgccgcgc
    28021 caacgccgat tcgctgccaa catgccgaac agactgcaca aaatgcgcca ttaaacctgc
    28081 aaacgctgga agccatcagc gaatcagtcc tgcaagatgt cgccccgcgt tcttcatggc
    28141 gggccagtaa agagtttcgt ctgcatctca tccagacgat gaccaaaaaa gtgattagcg
    28201 aagccgtcgc cgcggcgggg ggaaaattgc aatgaatcac agcgaaacaa ttaccatcga
    28261 atgcaccatt aacgggatgc cttttcagct tcacgccgcg ccaggaatgc cgctttcgga
    28321 actactccga gaacaagggc ttcttagtgt caaacaaggt tgctgcgtag gcgaatgcgg
    28381 tgcctgtacg gtgctggtcg acggcactgc gatagacagt tgcttattcc ttgcgacctg
    28441 ggctgaagga aaagagatcc gcacgctgga aggtgaagcg aaaggcggta aactttctca
    28501 tgtccaactg gcttatgcga aatctggtgc agtgcaatgc gggttttgta cgccgggcct
    28561 gattatggct accacggcga tgctggcaaa accacgcgaa aaaccattaa ccattacgga
    28621 aattcgtcgt ggactggcgg gaaatctttg tcgctgcacg gggtatcaga tgattgtaaa
    28681 tacagttctg gattgcgaga aaacgaagta aaaggatatc cggcctgaat tcaggccgga
    28741 ttctcagagg ttatgtgttt aacaactcat atttcttaat cttgcgatag agcgtagcaa
    28801 tgccgatgcc cagttcatca gcaacgtcct tcttgctgtt atgacgtgaa agcgcctcgc
    28861 ggatcatttg cttttccatc tcctccagcg ccgtgccgcc cgcatcatcg agtgacaggt
    28921 gcgcctcact gacctctgtt acatcacttt gctccgttgt gccattattc agcagatttg
    28981 gcggcaatag cgtgctgtcg ataacttcac ctgaaggaac aacgttaacc agatattcca
    29041 tcaaattgct taactcgcgc aggtttccgg gccaacgatg cttacgcaat atttcgacga
    29101 catcaggagc aatgccagga taaaccgatc ccagacgacg ggtatgcaga tgtaaaaagt
    29161 aatgcaccag taattcaata tcttcctgac gttcacgcag cggtggaaga gttatcggga
    29221 taacattaag tcggtagaag agatcttcgc ggaatttacc ttcggcaatg aactgggcca
    29281 aattctgatt agttgcagaa atgatgcgga tgtcgacttg tattgggcta ctggcaccaa
    29341 tcggcagaat ttcacgcgcc tcaatagcgc gcagtaattt agcctgcaac attaatggca
    29401 tatcaccaat ttcatcgaga aacagcgtgc ccgtattcgc cgcctgaatc aaccctgttt
    29461 taccgttggc agaagcgcca gtaaatgcgc ctttaacata accgaacagt tcgctctcca
    29521 gaagctgttc cggaatcgcg gcacagttga tagcaataaa gggtttattc cgtcttccgc
    29581 tcaacttatg gattgcacgg gcgacgactt ctttacccgt gccgctttca ccaaccacca
    29641 taacgctgga tgggctgggt gcaatacggc taatgagtcg ttttaattgc cgcataacac
    29701 ggcactcgcc aaccaattgt tcaatatgcg gttcatcaga tgcatttgct acagaaaaac
    29761 tggtatgcga ttggtgaaac gccattaaaa atagttgtcg gccctggatg ttatgcaact
    29821 gaccaatgat taattcactt ttatcatccc acgaaacaat atgttgcata tgaccatggg
    29881 taaaattact ctcaaatgtt aatggtctga aacgaacagg ttttccaata atattatttt
    29941 gtacaacacc gagtgttttt aaagctgtct gattaacaaa ctgaacacga ttatcagcat
    30001 caaccactaa cacgccctga tccatattat cgatcatggt cgcgaatatt ttactgatgt
    30061 tatctcctgg tccctgatcc tccagaagtt tagaaacaaa aatggtggat atatggcgaa
    30121 cgtaatcgga aaattcgcgt aaattatcac tgatatgttc ttgttgctcg tgggtaacgg
    30181 caatcaaact tatcacccca acacaacgat cctgtaaaat gacaggcgta cccagaaatg
    30241 ctttttcgcg gcaattttct ttactatcgc aaccttcaca aaggggatcg aagcgagact
    30301 gtgtcacaac tttttcagtt ttcgtttcca ggacgtggcg gagcaggcgt gagttgccgc
    30361 tcaactggcg gccaagaaac ttcccatacg cgcccgttcc ggcgacgcga cacaagtttt
    30421 catcaacgat ctcaacctca agctgcaaaa cgctggcaag cattccggca aaacgctgaa
    30481 ttgtcggttg aatttgcatc aatactgact gcgtagtagc aagctccata gctttacctt
    30541 ccagacttac ttaaaagtcg atcattgaag acgttgatgg ttcatagatc atgatgatat
    30601 taactcaggc aaaattggct ttgataaaaa cataagat
//
LOCUS       DAZFJL010000048        29854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFJL010000048 DAZFJL010000000
VERSION     DAZFJL010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.048"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..144
                     /gene="tuf"
                     /locus_tag="ACHJ8C_002087"
     CDS             <1..144
                     /gene="tuf"
                     /locus_tag="ACHJ8C_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO5551944.1"
                     /translation="VEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAK
                     VLS"
     gene            374..757
                     /gene="secE"
                     /locus_tag="ACHJ8C_002088"
     CDS             374..757
                     /gene="secE"
                     /locus_tag="ACHJ8C_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO5551945.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            759..1304
                     /gene="nusG"
                     /locus_tag="ACHJ8C_002089"
     CDS             759..1304
                     /gene="nusG"
                     /locus_tag="ACHJ8C_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO5551946.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEELFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1463..1891
                     /gene="rplK"
                     /locus_tag="ACHJ8C_002090"
     CDS             1463..1891
                     /gene="rplK"
                     /locus_tag="ACHJ8C_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO5551947.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1895..2599
                     /gene="rplA"
                     /locus_tag="ACHJ8C_002091"
     CDS             1895..2599
                     /gene="rplA"
                     /locus_tag="ACHJ8C_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO5551948.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDISEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAE
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     IRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2891..3388
                     /gene="rplJ"
                     /locus_tag="ACHJ8C_002092"
     CDS             2891..3388
                     /gene="rplJ"
                     /locus_tag="ACHJ8C_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO5551949.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3455..3820
                     /gene="rplL"
                     /locus_tag="ACHJ8C_002093"
     CDS             3455..3820
                     /gene="rplL"
                     /locus_tag="ACHJ8C_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO5551950.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4140..8168
                     /gene="rpoB"
                     /locus_tag="ACHJ8C_002094"
     CDS             4140..8168
                     /gene="rpoB"
                     /locus_tag="ACHJ8C_002094"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO5551951.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8245..12468
                     /gene="rpoC"
                     /locus_tag="ACHJ8C_002095"
     CDS             8245..12468
                     /gene="rpoC"
                     /locus_tag="ACHJ8C_002095"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO5551952.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYTIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHLINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12710..13843)
                     /gene="thiH"
                     /locus_tag="ACHJ8C_002096"
     CDS             complement(12710..13843)
                     /gene="thiH"
                     /locus_tag="ACHJ8C_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO5551953.1"
                     /translation="MKTFSDRWRQLDWDDIHLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAEAEYAELKQLGLDGVMVYQETYHEATYAHHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRP"
     gene            complement(13840..14610)
                     /gene="thiG"
                     /locus_tag="ACHJ8C_002097"
     CDS             complement(13840..14610)
                     /gene="thiG"
                     /locus_tag="ACHJ8C_002097"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO5551954.1"
                     /translation="MLHIADKTFDSHLFTGTGKFASSQLMVESIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFIVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14612..14812)
                     /gene="thiS"
                     /locus_tag="ACHJ8C_002098"
     CDS             complement(14612..14812)
                     /gene="thiS"
                     /locus_tag="ACHJ8C_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO5551955.1"
                     /translation="MQILFNDQPMQCVAGLTVHELLEQLDQQQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(14796..15551)
                     /gene="thiF"
                     /locus_tag="ACHJ8C_002099"
     CDS             complement(14796..15551)
                     /gene="thiF"
                     /locus_tag="ACHJ8C_002099"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO5551956.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLMA
                     LQQRLTGETLKDAVAQADVVLDCTDNMATRQEINATCVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLTLRRANGCPVCGGCNADPV"
     gene            complement(15544..16179)
                     /gene="thiE"
                     /locus_tag="ACHJ8C_002100"
     CDS             complement(15544..16179)
                     /gene="thiE"
                     /locus_tag="ACHJ8C_002100"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO5551957.1"
                     /translation="MYQPEFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLSAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16179..18074)
                     /gene="thiC"
                     /locus_tag="ACHJ8C_002101"
     CDS             complement(16179..18074)
                     /gene="thiC"
                     /locus_tag="ACHJ8C_002101"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO5551958.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGSAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEVFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDISLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18155..18255)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18307..18783)
                     /gene="rsd"
                     /locus_tag="ACHJ8C_002102"
     CDS             complement(18307..18783)
                     /gene="rsd"
                     /locus_tag="ACHJ8C_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO5551959.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDDYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKYPA"
     gene            18878..19651
                     /gene="nudC"
                     /locus_tag="ACHJ8C_002103"
     CDS             18878..19651
                     /gene="nudC"
                     /locus_tag="ACHJ8C_002103"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO5551960.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQLQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGEIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19691..20755
                     /gene="hemE"
                     /locus_tag="ACHJ8C_002104"
     CDS             19691..20755
                     /gene="hemE"
                     /locus_tag="ACHJ8C_002104"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO5551961.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVASILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20765..21436
                     /gene="nfi"
                     /locus_tag="ACHJ8C_002105"
     CDS             20765..21436
                     /gene="nfi"
                     /locus_tag="ACHJ8C_002105"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO5551962.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPSDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21479..22069
                     /gene="yjaG"
                     /locus_tag="ACHJ8C_002106"
     CDS             21479..22069
                     /gene="yjaG"
                     /locus_tag="ACHJ8C_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO5551963.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22256..22528
                     /gene="hupA"
                     /locus_tag="ACHJ8C_002107"
     CDS             22256..22528
                     /gene="hupA"
                     /locus_tag="ACHJ8C_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO5551964.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22541..23236
                     /gene="yjaH"
                     /locus_tag="ACHJ8C_002108"
     CDS             22541..23236
                     /gene="yjaH"
                     /locus_tag="ACHJ8C_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO5551965.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23238..23663)
                     /gene="zraP"
                     /locus_tag="ACHJ8C_002109"
     CDS             complement(23238..23663)
                     /gene="zraP"
                     /locus_tag="ACHJ8C_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO5551966.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGMGYGGCGGGGHMGMGHW"
     gene            23901..25277
                     /gene="zraS"
                     /locus_tag="ACHJ8C_002110"
     CDS             23901..25277
                     /gene="zraS"
                     /locus_tag="ACHJ8C_002110"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO5551967.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMMGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERASAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVELNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVN
                     ITRKDPQG"
     gene            25274..26599
                     /gene="zraR"
                     /locus_tag="ACHJ8C_002111"
     CDS             25274..26599
                     /gene="zraR"
                     /locus_tag="ACHJ8C_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO5551968.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSVDAETPAVSASQFSMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26596..27885)
                     /gene="purD"
                     /locus_tag="ACHJ8C_002112"
     CDS             complement(26596..27885)
                     /gene="purD"
                     /locus_tag="ACHJ8C_002112"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO5551969.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVRDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(27897..29486)
                     /gene="purH"
                     /locus_tag="ACHJ8C_002113"
     CDS             complement(27897..29486)
                     /gene="purH"
                     /locus_tag="ACHJ8C_002113"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO5551970.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGLDDAIMEEHQILPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DANEGSLMLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPSEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         6987 a         7752 c         8223 g         6892 t
ORIGIN      
        1 gtagagatgg taatgccggg cgacaacatc aaaatggttg ttaccctgat ccacccgatc
       61 gcgatggacg acggtctgcg tttcgcaatc cgtgaaggcg gccgtaccgt tggcgcgggc
      121 gttgtagcaa aagttctgag ctaattgccg ataacatttg acgcaatgcg cactaaaagg
      181 gcatcatttg atgccctttt tgcacgcttt cgtaccagaa cctggctcat cagtgatttt
      241 ctttgtcata atcattgctg agacaggctc tgttgagggc gtataatccg aaaagctaat
      301 acgcgtttcg atttggtttg cctcgcgatc gcggggtgaa aatgtttgta gaatacttct
      361 gacaggttgg tttatgagtg cgaataccga agctcaagga agcgggcgcg gcctggaagc
      421 gatgaagtgg gtcgttgtgg tggcattgct cctggtagcg attgtcggca actaccttta
      481 tcgcgacatt atgctgccgc tgcgtgcgct ggccgtagta attctgattg ctgcagcggg
      541 tggtgtcgcg ctgttaacga caaaaggtaa agcaaccgtt gcttttgccc gtgaagcgcg
      601 taccgaagtc cgtaaggtca tttggccgac tcgccaggaa acattgcaca ccacgctgat
      661 tgtggctgcg gttaccgcag taatgtcact gatcctgtgg ggactggatg gtattctggt
      721 tcgcctggta tcctttatca ctggcctgag gttctgagat gtctgaagct cctaaaaagc
      781 gctggtacgt cgttcaggcg ttttccggtt ttgaaggccg cgtagcaacg tcgctgcgtg
      841 agcatatcaa attacacaac atggaagagt tgtttggtga agtcatggta ccaaccgaag
      901 aagtggttga aatccgtggc ggtcagcgtc gcaaaagcga acgtaaattc ttccctggct
      961 acgtcctcgt tcagatggtg atgaacgacg cgagctggca cctggtgcgc agcgtaccgc
     1021 gtgtgatggg cttcatcggc ggtacttccg atcgtcctgc gccaatcagc gataaagaag
     1081 tcgatgcgat tatgaaccgc ctgcagcagg ttggtgataa gccgcgtccg aaaacgttgt
     1141 ttgaaccggg tgaaatggtc cgtgttaatg atggtccgtt cgctgacttc aacggcgttg
     1201 ttgaagaagt tgattacgag aaatctcgtc tgaaagtgtc tgtttctatc ttcggtcgtg
     1261 cgacccctgt agagctggac ttcagccagg ttgaaaaagc ttaacccagc gatcaaaaaa
     1321 gcggcgattt aatcgttgca caaggcgcga gattggaata caatttcgcg ccttttgttt
     1381 ttatgggcct tgcccgtaaa acgatttttt atatcacggg gagcctctca gaggcgttat
     1441 tacccaactt gaggaattta taatggctaa gaaagtacaa gcctatgtca agctgcaggt
     1501 tgcagctggt atggctaacc cgagtccgcc agtaggtccg gctctgggtc agcagggcgt
     1561 aaacatcatg gaattctgca aagcgttcaa cgcaaaaact gattccatcg aaaaaggtct
     1621 gccgattccg gtagtaatca ccgtttacgc tgaccgttct ttcactttcg ttaccaagac
     1681 tccgccggca gcagttctgc tgaaaaaagc ggctggtatc aagtctggtt ccggtaagcc
     1741 gaacaaagac aaagtgggta aaatttcccg cgctcagctg caggaaatcg cgcagaccaa
     1801 agctgccgac atgactggtg ccgacattga agcgatgact cgctccatcg aaggtactgc
     1861 acgttccatg ggcctggtag tggaggacta agaaatggct aaactgacca agcgcatgcg
     1921 tgttatccgc gagaaagttg atgcaaccaa acagtacgac atcagcgaag ctatcgctct
     1981 gctgaaagag ctggcgactg ctaaattcgt agaaagcgtg gacgtagctg ttaacctcgg
     2041 catcgacgct cgtaaatctg accagaacgt acgtggtgca actgtactgc cgcacggtac
     2101 tggccgttcc gttcgcgtag ccgtatttac tcagggtgca aatgctgaag ctgctaaagc
     2161 tgcaggtgct gaactggtag gtatggaaga tctggctgaa cagatcaaga aaggcgaaat
     2221 gaactttgac gttgttattg cttctccgga tgcaatgcgc gttgttggcc agctgggcca
     2281 ggttctgggt ccgcgcggcc tgatgccaaa cccgaaagtg ggtactgtaa cgccgaacgt
     2341 tgctgaagcg gttaaaaacg ctaaagctgg ccagattcgt taccgtaacg acaaaaacgg
     2401 catcatccac accaccatcg gtaaagtgga ctttgacgct gacaaactga aagaaaacct
     2461 ggaagctctg ctggttgcgc tgaaaaaagc aaaaccgact caggcgaaag gcgtgtacat
     2521 caagaaagtt agcatctcta ccaccatggg tgcaggtgtt gcagttgacc aggctggcct
     2581 gagcgcttct gtaaactaat gcctttacgt gggcggtgat tttgtctaca atcttacccc
     2641 cacgtatgct aacgaaagtt agcagctaaa aagatttgtt cgttggagcc tggcctatcc
     2701 aggcctccgt cgaagaccgc aggagtttcg caagaaactt aatcccctgc gtagacggtg
     2761 acagaacgct aagattattc ttttatattc tggcttgttt ctgctcaccg taattaagac
     2821 gctctctccg tttggaggag tgaagtgagt tccagagatt ttctctggca aacatccagg
     2881 agcaaagcta atggctttaa atcttcaaga caaacaagcg attgttgctg aagtcagcga
     2941 agtagccaaa ggcgcgctgt ctgcagtagt tgcggattcc cgtggcgtaa ctgtagataa
     3001 aatgactgaa ctgcgtaaag caggtcgcga agctggcgta tacatgcgtg ttgttcgtaa
     3061 caccctgctg cgccgtgctg ttgaaggtac tccgttcgag tgcctgaaag acgcgtttgt
     3121 tggtccgacc ctgattgcat actctatgga acacccgggc gctgctgctc gtctgttcaa
     3181 agagttcgcg aaagcgaatg caaaatttga ggtcaaagcc gctgcctttg aaggtgagct
     3241 gatcccggcg tctcagatcg accgcctggc aactctgccg acctacgaag aagcaattgc
     3301 acgcctgatg gcaaccatga aagaagcttc ggctggcaaa ctggttcgca ctctggctgc
     3361 tgtacgcgat gcgaaagaag ctgcttaatc gcagttatct ttttaacgca ttcgcttacg
     3421 tataaactta ttctgatatt caggaacaat ttaaatgtct atcactaaag atcaaatcat
     3481 tgaagcagtt gcagctatgt ctgtaatgga cgttgtagaa ctgatctctg caatggaaga
     3541 aaaattcggt gtttccgctg ctgctgctgt agctgtagct gctggcccgg ttgaagctgc
     3601 tgaagaaaaa actgaattcg acgtgattct gaaagctgct ggcgctaaca aagttgctgt
     3661 tatcaaagca gtacgtggcg caactggcct gggtctgaaa gaagctaaag acctggttga
     3721 atctgcaccg gctgctctga aagaaggcgt gagcaaagac gacgcagaag cactgaaaaa
     3781 agctctggaa gaagctggcg ctgaagttga agttaaataa gccaaccctt ccggttgcag
     3841 cctgagaaat caggctgatg gctggtgact ttttagtcac cagccttttt gcgctgtaag
     3901 gcgccagtag cgtttcacac tgtttgacta ctgctgtgcc tttcaatgct tgtttctatc
     3961 gacgacttaa tatactgcga caggacgccc gttctgtgta aatcgcaatg aaatggttta
     4021 agcgtgatag caacaggcat tgcggaaagt gttccatttt ccggtcaaca aaatagtgtt
     4081 gcacaaactg tccgctcaat ggacagatgg gtcgacttgt cagcgagctg aggaacccta
     4141 tggtttactc ctataccgag aaaaaacgta ttcgtaagga ttttggtaaa cgtccacaag
     4201 ttctggatgt accttatctc ctttctatcc agcttgactc gtttcagaaa tttatcgagc
     4261 aagatcctga agggcagtat ggtctggaag ctgctttccg ttccgtattc ccgattcaga
     4321 gctacagcgg taattccgag ctgcaatacg tcagctaccg ccttggcgaa ccggtgtttg
     4381 acgtccagga atgtcaaatc cgtggcgtga cctattccgc accgctgcgc gttaaactgc
     4441 gtctggtgat ctatgagcgc gaagcgccgg aaggcaccgt aaaagacatt aaagaacaag
     4501 aagtctacat gggcgaaatt ccgctcatga cagacaacgg tacctttgtt atcaacggta
     4561 ctgagcgtgt tatcgtttcc cagctgcacc gtagtccggg cgtcttcttt gactccgaca
     4621 aaggtaaaac ccactcttcg ggtaaagtgc tgtataacgc gcgcatcatc ccttaccgtg
     4681 gttcctggct ggacttcgaa ttcgatccga aggacaacct gttcgtacgt attgaccgtc
     4741 gccgtaaact gcctgcgacc atcattctgc gtgccctgaa ctacaccaca gagcagatcc
     4801 tcgacctgtt ctttgaaaaa gttatctttg aaatccgtga taacaagctg cagatggaac
     4861 tggtgccgga acgcctgcgt ggtgaaaccg catctttcga catcgaagct aacggtaaag
     4921 tgtacgtaga aaaaggccgc cgtatcactg cgcgccacat tcgccagctg gaaaaagacg
     4981 acgtcaaact gatcgaagtc ccggttgagt acatcgcagg taaagtggtt gctaaagact
     5041 atattgatga gtctaccggc gagctgatct gcgcagcgaa catggagctg agcctggatc
     5101 tgctggctaa gctgagccag tctggtcata agcgtatcga aacgctgttc accaacgatc
     5161 tggatcacgg cccatatatc tctgaaacct tacgtgtcga cccaactaac gaccgtctga
     5221 gcgcactggt agaaatctac cgtatgatgc gccctggcga gccgccgact cgtgaagcag
     5281 ctgaaagcct gtttgagaac ctgttcttct ccgaagaccg ctatgacctg tctgcggttg
     5341 gtcgtatgaa gttcaaccgt tctctgctgc gcgaagaaat cgaaggttcc ggtatcctga
     5401 gcaaagacga catcattgat gttatgaaaa agctcatcga tatccgtaac ggtaaaggcg
     5461 aagtcgatga tattgaccac ctcggcaacc gtcgtatccg ttccgttggc gaaatggcgg
     5521 aaaaccagtt ccgcgttggc ctggtgcgtg tagagcgtgc ggtgaaagag cgtctgtctc
     5581 tgggcgatct ggataccctg atgcctcagg atatgatcaa cgccaagccg atttccgcag
     5641 cagtgaaaga gttcttcggt tccagccagc tgtctcagtt tatggaccag aacaacccgc
     5701 tgtctgagat tacgcacaaa cgtcgtatct cggcccttgg cccaggcggt ctgacccgtg
     5761 aacgtgcagg cttcgaagtt cgagacgtac acccgactca ctacggtcgc gtatgtccaa
     5821 tcgaaacccc tgaaggtccg aacatcggtc tgatcaactc cctgtccgtg tacgcacaga
     5881 ctaacgaata cggcttcctt gagactccgt atcgtaaagt gaccgacggt gttgtaactg
     5941 acgaaattca ctatctgtct gctatcgaag aaggcaacta cgttatcgcc caggcgaact
     6001 ccaacctgga tgaagaaggc cacttcgtag aagacctggt aacctgccgt agcaaaggcg
     6061 aatccagttt gttcagccgc gaccaggttg attacatgga cgtatccacc cagcaggtgg
     6121 tatccgtcgg tgcgtccctg atcccgttcc tggaacacga tgacgccaac cgtgcattga
     6181 tgggtgcgaa catgcaacgc caggccgttc cgactctgcg tgctgataag ccgctggttg
     6241 gtacgggtat ggaacgtgct gttgccgttg actccggtgt aactgcggtt gctaaacgtg
     6301 gtggtgtcgt tcagtacgtg gatgcttccc gtatcgttat caaagttaac gaagacgaga
     6361 tgtatccggg tgaagcaggt atcgacatct acaacctgac caagtacacc cgttctaacc
     6421 agaacacctg tatcaaccag atgccgtgtg tgtctctggg tgaaccggta gaacgtggcg
     6481 acgtgctggc agacggtccg tccaccgacc tcggtgaact ggcgcttggt cagaacatgc
     6541 gcgtagcgtt catgccgtgg aatggttaca acttcgaaga ctccatcctc gtatccgagc
     6601 gtgttgttca ggaagaccgt ttcaccacca tccacattca ggaactggcg tgtgtgtccc
     6661 gtgacaccaa gctggggcca gaagagatca ccgctgacat cccgaacgtg ggtgaagctg
     6721 cgctctccaa actggatgaa tccggtatcg tttacattgg tgcggaagtg accggtggcg
     6781 acattctggt tggtaaggta acgccgaaag gtgaaactca gctgacccca gaagagaaac
     6841 tgctgcgtgc gatcttcggt gagaaagcgt ctgacgttaa agactcttct ctgcgcgtac
     6901 caaacggtgt atccggtacg gttatcgatg ttcaggtctt tactcgcgat ggcgtagaaa
     6961 aagacaaacg tgcgctggaa atcgaagaaa tgcagctcaa acaggcgaag aaagacctgt
     7021 ctgaagaact gcagatcctc gaagcaggtc tgttcagccg tatccgtgct gtgctggtag
     7081 ccggtggcgt tgaagctgag aagctcgaca aactgccgcg cgatcgctgg ctggagctgg
     7141 gcctgaccga cgaagagaaa caaaatcagc tggaacagct ggctgagcag tatgacgaac
     7201 tgaaacacga gttcgagaag aaactcgaag cgaaacgccg caaaatcacc cagggcgacg
     7261 atctggcacc gggcgtgctg aagattgtta aggtatatct ggcggttaaa cgccgtatcc
     7321 agcctggtga caagatggca ggccgtcatg gtaacaaggg tgtaatttct aagatcaacc
     7381 cgatcgaaga tatgccttac gatgaaaacg gtactccggt agacatcgta ctgaacccgc
     7441 tgggcgtacc gtctcgtatg aacatcggtc agatcctcga aacccacctg ggtatggctg
     7501 cgaaaggtat cggcgacaag atcaacgcca tgctgaaaca gcagcaagaa gtcgcgaaac
     7561 tgcgcgaatt catccagcgt gcgtacgatc tgggcgctga cgttcgtcag aaagttgacc
     7621 tgagtacctt cagcgatgaa gaagttatgc gtctggctga aaacctgcgc aaaggtatgc
     7681 caatcgcaac gccagtgttc gacggtgcga aagaagcaga aattaaagag ctgctgaaac
     7741 ttggcgacct gccgacttct ggtcagatcc gcctgtacga cggtcgtact ggtgaacagt
     7801 tcgaacgtcc ggtaaccgtt ggttacatgt acatgctgaa actgaaccac ctggtcgacg
     7861 acaagatgca cgcgcgttcc accggttctt acagcctggt tactcagcag ccgctgggtg
     7921 gtaaggcaca gttcggtggt cagcgtttcg gggagatgga agtgtgggcg ctggaagcat
     7981 acggcgcagc atacaccttg caggaaatgc tcaccgttaa gtctgatgac gtgaacggtc
     8041 gtactaagat gtataaaaac atcgtggacg gcaaccatca gatggagccg ggcatgccag
     8101 aatccttcaa cgtattgttg aaagagattc gttcgctggg tatcaacatc gaactggaag
     8161 acgagtaatt ctcgctcaaa caggtcactg ctgtcggggt aaaacccggc agcggattgt
     8221 gctaactccg acgggagcaa atccgtgaaa gatttattaa agtttctgaa agcgcagact
     8281 aaaaccgaag agtttgatgc gatcaaaatt gctctggctt cgccagacat gatccgttca
     8341 tggtctttcg gtgaagttaa aaagccggaa accatcaact accgtacgtt caaaccagaa
     8401 cgtgacggcc ttttctgcgc ccgtatcttt gggccggtaa aagattacga gtgcctgtgc
     8461 ggtaagtaca agcgcctgaa acaccgtggc gtcatctgtg agaagtgcgg cgttgaagtg
     8521 acccagacta aagtacgccg tgagcgtatg ggccacatcg aactggcttc cccgactgcg
     8581 cacatctggt tcctgaaatc gctgccgtcc cgtatcggcc tgctgctcga tatgccgctg
     8641 cgcgatatcg aacgtgtact gtacttcgaa tcctatgtgg ttatcgaagg tggtatgacc
     8701 aacctggagc gtcagcagat cctgactgaa gagcagtatc tggacgcgct ggaagagttc
     8761 ggtgacgaat tcgacgcgaa gatgggtgcg gaagcaatcc aggccctgct gaagagcatg
     8821 gatctggagc aagagtgcga acagctgcgt gaagagctga acgaaaccaa ctccgaaacc
     8881 aagcgtaaaa agctgaccaa gcgtatcaaa ctgctggaag cgttcgttca gtctggtaac
     8941 aaaccagagt ggatgatcct gaccgttctg ccggtactgc cgccagatct gcgtccgctg
     9001 gttccgctgg atggtggtcg tttcgcgact tctgacctga acgatctgta tcgtcgcgtc
     9061 attaaccgta acaaccgtct gaaacgtctg ctggatctgg ctgcgccgga tatcatcgta
     9121 cgtaacgaaa aacgtatgct gcaggaagcg gtagacgccc tgctggataa cggtcgtcgc
     9181 ggtcgtgcaa tcaccggttc taacaagcgt cctctgaaat ctttggccga catgatcaaa
     9241 ggtaaacagg gtcgtttccg tcagaacctg ctcggtaagc gtgttgacta ctccggtcgt
     9301 tctgtcatca ccgtaggtcc atacctgcgt ctgcatcagt gcggtctgcc gaagaaaatg
     9361 gcgctggagc tgttcaaacc gttcatctac ggcaagctgg aactgcgtgg tcttgctacc
     9421 accatcaaag ctgcgaagaa aatggttgag cgcgaagaag ctgtcgtttg ggatatcctg
     9481 gacgaagtta tccgcgaaca cccggtactg ctgaaccgtg caccgactct gcaccgtctg
     9541 ggtatccagg catttgaacc ggtactgatc gaaggtaaag ctatccagct gcacccgctg
     9601 gtttgtgcgg catataacgc cgacttcgat ggtgaccaga tggctgtgca cgtaccgctg
     9661 acgctggaag cccagctgga agcgcgtgcg ctgatgatgt ctaccaacaa catcctgtcc
     9721 ccggcgaacg gcgaaccaat catcgttccg tctcaggacg ttgtactggg tctgtactac
     9781 atgacccgcg actgtgttaa cgccaaaggc gaaggcatgg tgctgactgg cccgaaagaa
     9841 gcagaacgtc tgtatcgctc tggtctggct tctctgcatg cgcgcgttaa agtgcgtatc
     9901 accgagtatg aaaaagatgc taacggtgaa ttagtggcga aaaccagcct gaaagacacg
     9961 actgttggcc gtgccattct gtggatgatt gtaccgaaag gtctgcctta caccatcgtc
    10021 aaccaggcgc tgggtaaaaa agcaatctcc aaaatgctga acacctgcta ccgcattctc
    10081 ggtctgaaac cgaccgttat ttttgcggac cagatcatgt ataccggctt tgcatatgca
    10141 gcgcgttctg gtgcatctgt tggtatcgat gacatggtca tcccggagaa gaaacacgaa
    10201 atcatctccg aggcagaagc agaagttgct gaaattcagg agcagttcca gtctggtctg
    10261 gtaactgcgg gcgaacgcta caacaaagtt atcgatatct gggctgcggc gaacgatcgt
    10321 gtatccaaag cgatgatgga taacctgcaa actgaaaccg ttattaaccg tgacggtcag
    10381 gaagagaagc aggtttcctt caacagcatc tacatgatgg ccgactccgg tgcgcgtggt
    10441 tctgcggcac agattcgtca gcttgctggt atgcgtggtc tgatggcgaa gccggatggc
    10501 tccatcatcg aaacgccaat caccgcgaac ttccgtgaag gtctgaacgt actccagtac
    10561 ttcatctcca cccacggtgc tcgtaaaggt ctggcggata ccgcactgaa aacggcgaac
    10621 tccggttacc tgactcgtcg tctggttgac gtggcgcagg acctggtggt taccgaagac
    10681 gattgtggta cccatgaagg tatcatgatg actccggtta tcgagggtgg tgacgttaaa
    10741 gagccgctgc gcgatcgcgt attgggtcgt gtaactgctg aagacgttct gaagccgggt
    10801 actgctgata tcctcgttcc gcgcaacacg ctgctgcacg aacagtggtg tgacctgctg
    10861 gaagagaact ctgtcgacgc ggttaaagta cgttctgttg tatcttgtga caccgacttt
    10921 ggtgtatgtg cgcactgcta cggtcgtgac ctggcgcgtg gccacctcat caacaaaggt
    10981 gaagcaatcg gtgttatcgc ggcacagtcc atcggtgaac cgggtacaca gctgaccatg
    11041 cgtacgttcc acatcggtgg tgcggcatct cgtgcggctg ctgaatccag catccaggtg
    11101 aaaaacaaag gtagcatcaa gctcagcaac gtgaagtcgg ttgtgaactc cagcggtaaa
    11161 ctggttatca cttcccgtaa caccgaactg aaactgatcg acgaattcgg tcgtaccaaa
    11221 gaaagctaca aagtacctta cggtgcggta ctggcgaaag gcgatggcga acaggttgct
    11281 ggcggcgaaa ccgttgcaaa ctgggacccg cacaccatgc cggttatcac cgaagtaagc
    11341 ggttttgtac gctttactga catgatcgac ggccagacca ttactcgtca gaccgacgaa
    11401 ttgacaggtc tgtcttcgct ggtggttctg gattccgcag aacgtaccgc aggtggtaaa
    11461 gatctgcgtc cggcactgaa aatcgttgat gctcagggta acgacgttct gatcccgggt
    11521 accgatatgc ctgcgcagta cttcctgccg ggtaaagcga ttgttcagct ggaagatggc
    11581 gtacagatca gctctggtga caccctggcg cgtattccgc aggaatccgg cggtaccaag
    11641 gacatcaccg gtggtctgcc gcgcgttgcg gacctgttcg aagcacgtcg tccgaaagag
    11701 ccggcaatcc tggctgaaat cagcggtatc gtttccttcg gtaaagaaac caaaggtaaa
    11761 cgtcgtctgg ttatcacccc ggtagacggt agcgatccgt acgaagagat gattccgaaa
    11821 tggcgtcagc tcaacgtgtt cgaaggtgaa cgtgtagaac gtggtgacgt aatttccgac
    11881 ggtccggaag cgccgcacga cattctgcgt ctgcgtggtg ttcatgctgt gactcgttac
    11941 atcgttaacg aagtacagga cgtataccgt ctgcagggcg ttaagattaa cgataaacac
    12001 atcgaagtta tcgttcgtca gatgctgcgt aaagctacca tcgttaacgc gggcagctcc
    12061 gacttcctgg aaggtgaaca ggttgaatac tctcgcgtca agatcgcaaa ccgcgaactg
    12121 gaagcgaacg gcaaagtggg tgcaacttac tcccgcgacc tgctgggtat caccaaagcg
    12181 tctctggcaa ccgagtcctt catctccgcg gcatcgttcc aggagaccac tcgcgtgctg
    12241 accgaagcag ccgttgcggg caaacgcgat gaactgcgcg gcctgaaaga gaacgttatc
    12301 gtgggtcgtc tgatcccggc aggtaccggt tacgcgtacc accaggatcg tatgcgtcgc
    12361 cgtgctgcgg gtgaagctcc ggctgcaccg caggtgactg cagaagacgc atctgccagc
    12421 ctggcagaac tgctgaacgc aggtctgggc ggttctgata acgagtaatc gttaatccgc
    12481 aaataacgta aaaacccgct tcggcgggtt tttttatggg tgttattcag agggcaaagt
    12541 cttcacggaa aatattaaga aaggcagggc gaaacgagga agaagctttt cgccctggac
    12601 aacattcctg ctgacgggac taccataaaa aaacacaacg ctttcaacaa ccttgcgttt
    12661 ttcatcttcc gtagcggctc ggaataaatt tacgttctta cattctgtct catggccttt
    12721 gcgaggcgcg tcccagatag ctgtcccagt ctttccatac cggctgcaaa ccctgagcag
    12781 ttaacgcggc cgcaactgct tctggtctgc gatcgtcgtg cggtgagaac tgttccagct
    12841 cggggtgatt gtcggcatag ccacctggct gcgttttcga aaaagcgctg acgttattaa
    12901 ttgccagcgg aataacgcga tcgcgaaacc acggtgattc ccgcgtggag agtgacagtt
    12961 caatctccgg tgcaagcagc cggaaggcgc agatggtttg cactaactgg cgttcatcca
    13021 taatcgacgc aggctcaatg ccgccagtac acgggcgcag gcgtgggaag gagacagagt
    13081 agcggctttg ccagtagtgc tgttgcagcc atagcaaatg ttctgcaacc atatagcagt
    13141 caacgcgcca gctgtcggaa aggccaatta gcgcgccgag gcctatctta tcaatccccg
    13201 cacggcccag ccgatccggc gtttccagcc gccagaagaa gtcctgtttt ttacctttca
    13261 gatgatgatg ggcataagtc gcctcatgat atgtctcctg ataaaccatt actccatcga
    13321 ggccaagctg ttttaactcg gcatattccg cctccgccag cggttgcact tccatctgta
    13381 gtgaagagaa ctgttcacgc agggcaggga gatgacgacg aaagtaatcc atccccactt
    13441 tcgcctgatg ttcaccagtg actaatagca gatgttcaaa gcccatctcc cgtatagcgg
    13501 cgctttccct ggcaatatcc gcttcatcca gcgttttgcg cttgatgcgg ttgctcatag
    13561 aaaatccgca ataagtacag tcgttagcgc aaagattgga aagataaagc gggacgtaga
    13621 agctgacgac gttgccaaat cgctgacgag tcagacgttg cgctcgctgg gccagtggtt
    13681 ccagatagcc actggcggcg ggtgatagca atgccatcat gtcgtcgcgg gtgagttgcg
    13741 aggcatttag cgcccgctct acgtcagcag ccgttttgcc gttgatacgc aggtggatgt
    13801 catcccagtc cagttgtcgc cagcgatcgc tgaaggtttt catgccgatg cctccagaaa
    13861 tccagtcagc gggctggtgg catgagcaaa atgactgcgg ctgcccggtc cggactgacg
    13921 tgccagcagg cctgcttcta ccgccagacg aaatgccttc gccatgttga cgggatcgtc
    13981 cgcgacggca atcgccgtat tcactaacac cgcgtcggcc cccatttcca gcgcctgcgc
    14041 ggcatggctg ggaacgccga tgccagcatc gacaaccacc ggaacggtgg cctgctggat
    14101 aataatctcc agcatggcgc gggtttccag tccctgattc gagccaatcg gcgcgccgag
    14161 cggcatcacc gctgcacagc cgacttcttc cagacgttta cacaataccg gatcggcccc
    14221 gcagtaaggc agcacgataa atccctgttg taccagcgtt tcggcggctt tcagggtttc
    14281 gatgggatcg ggcaacagcc agcgggcgtc agggtgaatc tccaatttta gccagtttgt
    14341 gcctaacgct tcacgtgcca gatgggcggc gaaaatggct tcttccgcgg ttttcgcccc
    14401 ggaagtattc ggcagcaggg tcacacccgc cgcgataagc ggttcgagga tagcgtcgtt
    14461 gtgctggcgc aagtcgacac gtttcatcgc cagcgtcacc agttggctgc cggaagcgcg
    14521 aatagactcc accatcagtt gtgaagaggc gaattttccg gtgccagtaa acagatgtga
    14581 atcaaacgtt ttgtccgcaa tatgtaacat ctcaaccccc tgcaataact tgaaaaagca
    14641 ggatctggtc gccatcctgc acgatatgtt gcgcccattg ttcccgcggg acgatttgct
    14701 gattaatcgc tagtgccgca cctgcttgtt gttgatcgag ttgctccagc agttcgtgaa
    14761 cagttagtcc ggcgacacac tgcatcggtt gatcgttaaa caggatctgc attgcatcct
    14821 ccacataccg ggcaaccgtt ggcgcggcgc aacgtcaggc tgcgccactg gctcgattta
    14881 ccgtcgaaca gtcggagttc tcccgcaggc gtctctatac cgcttaataa cttaatcgct
    14941 tccagtgcct gtaaagtgcc cataaccccg accaccgggc caaccacgcc cgccgtgcgg
    15001 cagttgcgtt ctggctcctg gttatctggc cacaggcagc ggtaacatcc ctgctcccag
    15061 ggcggcgtca gtaccatcaa ctgaccgcca aatccgaccg cgctggcggt gataagcggc
    15121 gtgttgagtg ccacgcaggt ggcattaatc tcctggcgag tcgccatatt gtcagtacag
    15181 tcgagcacca catcggcttg tgcaaccgca tcttttaacg tctcacccgt taatcgttgt
    15241 tgtaatgcca tcagttgaat gtcgggattc aactgtgtca gtcgctgttg gctgacctgt
    15301 gatttcgggc gatcgatatc ttcagtggta aagaggattt gtcgttgcag attgcttaaa
    15361 tgcacatcgt cgtcatctgc cagtaccagc gtcccgacgc cagcccccgc caggtatagc
    15421 gcagcaggtg tacccagccc gccaagacca ataatcagca cctggctgtc gagcagtttt
    15481 tgctgcccgt ccagagcgat atcgtcgagc aggatttggc ggctataacg cataaagtca
    15541 cggtcattca tcgccaactc ctgcaatttc cagcagctgt gccgttgcca aacgccagtc
    15601 tgcggcttga gtaatggcgc tgacgacggc gatactgccg acacccgttg ctatcaccgc
    15661 aggcgcgcgc gccagactga taccgccaat cgccacggtg ggataatccg ccaggcgctc
    15721 aacatgccgt gccagctgtt ccagtccctg cggtgcagaa ggcatctgtt tggtttgtgt
    15781 cggaaacaca tgtcccagcg cgatataaga aggacgtgcc gccagcgcga cgtcgatctc
    15841 catatcgtca tgggtcgaaa cgcccagccg caggcctgcc gcgcggatgg cgctgagatc
    15901 ggtggcttgc aaatcttcct gccccagatg gacgccatac gcctgatgct taatcgccag
    15961 ccgccagtaa tcgttgataa acaatcgcgc gttatagcgg cggcccagcg caattgccgc
    16021 caccacatcg gcttctacct cttcatcgcg ccgatctttg atgcgtagct ggagagtacg
    16081 tacgcctgca tccaacagac gttcgatcca ctgtacgctg tcaaccaccg ggtacagtcc
    16141 taaacgaaag ggtacaggag gaaactcagg ctgatacatc acgcttcctc cttacgcagg
    16201 tagatttctc cgcctctggc gcggaagttc tccgacatat ccgccattcc cacttcaatg
    16261 gtttgcgcgg cggcgtaatc acgcacttcc tggctgattt tcatcgagca gaatttcggt
    16321 ccacacatgg agcaaaaatg ggcgacttta ccggactctt gcggcagggt ttcatcgtga
    16381 taagcgcggg cagtaaacgg gtcgagggcc agattaaact ggtcttccca gcgaaattcg
    16441 aagcgggctt tcgacatggc gttatcgcga atttgcgcgc ccggatgccc tttcgccagg
    16501 tcggcggcgt gagcggcaat cttataggtg ataagcccct gtttaacatc ttctttattt
    16561 ggcaggccca gatgctcttt cggcgttacg tagcagagca ttgcacaacc aaaccagcca
    16621 atcatcgccg cgccaatccc tgacgtgaag tggtcatagc ccggcgcgat atcggtagtt
    16681 agcggcccca gagtgtaaaa cggcgcttcg tggcagtgct ctaactcctc ggtcatattg
    16741 cggcgaatca tctgcatcgg cacgtggcct gggccttcaa tcatcacctg tacgtcatat
    16801 tcccaggcga ttttagtcag ttcacccagc gtatgcagct cggcaaactg tgcttcatcg
    16861 ttggcgtcct gaatagaacc ggggcgcagg ccgtcgccca gcgatagcga aatgtcataa
    16921 gcagcacaga tttcacaaat ttcgcggaag tgttgataga ggaaattttc ctgatgatgg
    16981 gagaggcacc atttcgccat aatcgaaccg ccgcgagaga cgataccggt caggcgtttc
    17041 gcggtcatcg gcacgtagcg cagcagcacc cccgcatgga tcgtgaagta atccacacct
    17101 tgctcggctt gttccagcag ggtgtcgcgg aagacttccc aggtaagatc ttcagcgatc
    17161 ccgttcacct tctccagtgc ctggtagatc ggcactgtac cgatcggcac cgggctgtta
    17221 cgcaaaatcc actcgcgggt ttcgtgaata tagcgaccag tggaaagatc catcacggta
    17281 tccgctcccc agcgtgtgga ccataccagt ttttccactt cttcttcgat ggaagaagtg
    17341 accgctgagt tgccgatatt ggcgttaact tttaccagga agttacggcc aataatcatc
    17401 ggctccgatt ccggatgatt aatgttggcc gggataatcg cacgtccggc agcaacttca
    17461 tcacggacaa attccgcagt gatattttcc ggcagacgtg cgccaaagct cattcccgga
    17521 tgctggtggc gtaaaacttc gctacgtatg cgctcgcgac ccatattctc gcgaatggcg
    17581 atgaattcca tttcgggcgt gatgataccc tggcgggcgt agtgcagttg ggtgacgcgg
    17641 cgtcctgctt tggcgcgttt tggcgttaat acgccgctaa aacgcagctc gtcgaggcca
    17701 tcatctgcca gccgcgcttt tgtgtaatcg gaactgcgca cggtaagttc ttcggtatcg
    17761 ccgcgcgcat cgatccacgg ctggcgtagt tttgccagcc cttgctgcac gttaatggca
    17821 atctgcggat cgccatatgg gccggaggtg tcgtagaccg gaatcgcttc gttttcttcg
    17881 tactgcggct gttctttgct gccgccaatt agcgtcgggc taagctggat ctcacgcatt
    17941 ggcacgcgca cgccgggttg tgtgccagtg atgtaaatgc gttttgagtt gggaaaggcg
    18001 gagccttcca gggtgtcgat aaaatgttgg gcctgggcgc gttgttcgcg gcgggtcagt
    18061 tttgttgcag acatagctca ttccaaaaag ttaaggacgt ggcttgtcag acgacggatg
    18121 aagcaagaga cgatcgccgc aaaggcgatg cgatagcaga ttaactcttg ttcccttcgc
    18181 aggtattagc ctgatcaggt tccgcggatc ccgaataaac ggtctcagcc agttaaggca
    18241 ctccgacaag aaattagccc cacaaacggg gcgttgaatg taaattacgc gttaacagcg
    18301 cagaactcaa gcaggatatt tgacgcgggc ggcgtcaagc accagcagaa tcagcttatc
    18361 ttccagcaca aagcgcgctt ccagtgcctc gccgatgtca gataaaacct gttgaaactc
    18421 aaggtaatta tcatgatcga tggcggtttc cagactggaa tcgtagtcat ccataatctg
    18481 ttgagtattg gcttcgagtt gtggccaaat cttcgcggcg cgtgcgagtt gcccattgcc
    18541 ttccagctta tgaagaatgc gctcataaat actgaaatgt ccggcagaca agtaatcgac
    18601 caggctctga caaaaatcat caagggcttt ttcgtttagc ctcatgtacg attctttgcc
    18661 aggcttaatg ccaaccagat tgtagtaagc cacgagcaga tgcttacgta catgtagcca
    18721 gcgatcaacc agtttgttac ttcctctgac gcgctccgtc aggttatcga gctggttaag
    18781 catgattgac tccgcaagtt tgtattcaaa aactgctcag tgagaaatgt aaaaaccatg
    18841 ttaaacatgc cagtgatgca aaggtagtgc aagagctatg gatcgtataa ttgaaaaatt
    18901 agatcacggc tggtgggtcg tcagccatga acaaaaatta tggttgccga agggagaatt
    18961 gccatatggc gaagcggcaa atttcgatct tgtgggtcag cgcgcactac aaatcggcga
    19021 atggcagggg gaacctgttt ggttagtaca actgcagcgg cgtcacgata tggggtcggt
    19081 acgtcaggtc attgatctcg atgttgggct gtttcaactg gccggacgtg gcgtgcaact
    19141 ggcggagttt taccgatcgc ataaatactg tggttactgc gggcatgaaa tgtatccaag
    19201 caaaactgaa tgggcgatgc tgtgcagcca ttgccgtgag cgttactacc cgcaaatcgc
    19261 cccctgcatt attgttgcca tccgtcgcga tgattcgatc ctcctcgccc agcatacccg
    19321 ccatcgtaac ggtgtccata cagtacttgc cggattcgtc gaagtgggtg aaaccctcga
    19381 gcaggcagtc gcgcgggaag tgatggaaga gagcggaatt aaagttaaaa acttgcgtta
    19441 cgtgacctcc caaccgtggc cgtttcctca gtctttaatg accgcgttta tggcggaata
    19501 tgacagcggc gagatcgtga tcgacccgaa agaattgctt gaggcgaact ggtatcgcta
    19561 tgacgatttg ccgttactcc cgccgcccgg caccgtagcg cgccgtctga tagaagatac
    19621 agtggcgatg tgtcgggcag agtatgagtg atgatacact gaccgcctga cgcactaagg
    19681 aacagccaaa atgaccgaac ttaaaaacga tcgttatctg cgggcgctgc tgcgccagcc
    19741 cgttgatgtc actccagtat ggatgatgcg ccaggcgggt cgctatctac cggaatataa
    19801 agccacgcgc gcccaggcgg gcgattttat gtcgctgtgc aaaaatgccg agctggcgtg
    19861 cgaagtgact ttgcaaccgc tgcgtcgcta tccgctggat gcggcgatcc tcttttccga
    19921 tatcctcacc gtgccggacg cgatgggttt agggctctat tttgaagccg gagaaggtcc
    19981 gcgttttacc tcgccagtca cctgcaaagc cgacgtcgat aaactgccaa ttccggaccc
    20041 agaagatgag ctgggttacg tgatgaacgc ggtgcgtacc attcgtcgcg aactgaaagg
    20101 cgaagtgccg ctgattggtt tttccggcag cccgtggacg ctggcgacct acatggtgga
    20161 aggcggcagc agcaaagcgt tcaccgtgat caaaaaaatg atgtatgccg atccacaggc
    20221 gctgcacgcg ctactcgata aactggcgaa aagcgtcact ttgtatctga atgcgcagat
    20281 taaagccggt gctcaggcag tcatgatttt cgacacctgg ggcggcgtgc ttaccgggcg
    20341 cgattatcaa cagttctcgc tttattacat gcataaaatt gttgatggtt tactgcgtga
    20401 aaacgacggt cgccgcgtac cggtcacgct gtttaccaaa ggtggcggac agtggctgga
    20461 agcgatggca gaaaccggtt gcgatgcgct gggcctcgac tggacaacgg acatcgccga
    20521 tgcgcgccgt cgcgtgggca ataaagtcgc gctgcagggt aatatggacc cgtcgatgct
    20581 gtacgcgccg cctgcccgca ttgaagaaga agtggcgtct atacttgccg gtttcggtca
    20641 cggcgaaggc catgtcttta accttggtca cggcattcat caggatgtgc cgccagaaca
    20701 tgcaggcgtg tttgtggagg cagtgcatcg cttgtctgaa cagtatcacc gctaaggagc
    20761 gattatggat ctcgcgtcat tacgcgctca acaaattgaa ctggcttctt ctgtgatccg
    20821 cgaggatcga ctcgataaag atccgtcgga tctgatcgcc ggagccgatg tcggatttga
    20881 gcagggcggc gaagtgacgc gagcggcgat ggtgctgctg aaatacccct cgcttgagct
    20941 ggtggagtat aaagtggccc gcatcgccac caccatgcct tacattcctg gttttctctc
    21001 ctttcgtgaa tatcctgcgc tgctggcagc gtgggagatg ctgtcacaaa agccggattt
    21061 agtgtttgtc gatggtcatg ggatctcgca tcctcgccgt cttggcgtcg ccagccattt
    21121 tggcttattg gtggatgtgc cgaccattgg cgtagcgaaa aaacggctct gcggtaaatt
    21181 cgaaccgctc tccagcgaac cgggcgcgct ggccccgctg atggataaag gcgagcagct
    21241 ggcctgggtc tggcgcagca aagcgcgctg taacccgttg tttatcgcta ccggccatcg
    21301 ggtcagcgtg gacagcgcgc tggcgtgggt acaacgctgc atgaaaggct atcgtctgcc
    21361 ggagccaacg cgctgggcgg acgcggtggc ctcggaacgt ccggcgttcg tgcgctatac
    21421 agcaaatcag ccctaattca ggtaaactgc ggataatttc cgtatttgag aactcaacat
    21481 gttacaaaac ccaattcatc tgcgtctgga gcgcctggaa agctggcagc acgtcacttt
    21541 tatggcctgc ttatgcgaac gtatgtaccc caattacgcc gtattctgtc agcaaaccgg
    21601 cttcggcgat gggcagattt accgccgcat tctcgatctc atttgggaaa cgctgaccgt
    21661 caaagacgca aaagtaaatt tcgacagcca actggagaaa tttgaagaag cgattccttc
    21721 agccgacgat ttcgatctgt acggcgttta tccggcaatc gatgcttgcg tggcgttaag
    21781 tgaactggtc cattcgcgtt taagtggcga aacgctcgaa cacgcggtgg aagtgagtaa
    21841 gacttccatc acgaccgtgg cgatgctgga aatgactcag gctggtcgcg aaatgagcga
    21901 tgaagagctc aaagaaaacc cagctgtaga gcaagaatgg gacattcagt gggaaatatt
    21961 ccgactttta gctgagtgcg aagaacgcga tatcgagctg ataaaaggcc ttagggcaga
    22021 cctgcgtgag gcgggtgaga gcaatattgg tataattttt cagcaataag accagaaaac
    22081 gtgatttaac gcctgatttg tcgtacctgg agtcttccct ttcgcccccc gtctggtcta
    22141 catttggggg gcgaaaaaaa gtggctatcg gtgcgtgtat gcaggagagt gctattctgg
    22201 catttccgtc gcactcgatg cttagcaagc gataaacaca ttgtaaggat aacttatgaa
    22261 caagactcaa ctgattgatg taattgcaga gaaagcagaa ctgtccaaaa cccaggctaa
    22321 agctgctctg gagtccactc tggctgcaat tactgagtct ctgaaagaag gcgatgctgt
    22381 acaactggtt ggtttcggta ccttcaaagt gaaccaccgc gctgagcgta ctggccgcaa
    22441 cccgcagacc ggtaaagaaa tcaaaattgc cgcagctaac gtaccggcat ttgtttctgg
    22501 caaggcactg aaagacgcag ttaagtaaga ttgcgtggca gtgaacagtt ttaacgaagg
    22561 ggtggtttca ccccttttgt ctttctggcg tcgatcattg atgctggctg gcgctctgtt
    22621 tctcactgcc tgtagtcaca actcttcact tcctcccttt accgccagtg gatttgctga
    22681 agaccagggc gcggtacgca tctggcgaaa agacagcggc gataatgtgc atcttcttgc
    22741 cgtgtttagc ccgtggcgca gtggcgatac cacgacgcga gagtatcgct ggcagggcga
    22801 taacctcacg ctcatcaata tcaatgttta cagcaaaccc ccggtgaata ttcgcgcgcg
    22861 ttttgacgat cgaggcgatc tgagctttat gcaacgcgaa tccgatgggg aaaagcagca
    22921 gctttctaac gaccaaatcg atttataccg ttatcgtgct gatcagatcc gccagattag
    22981 cgatgcctta cgtcagggga gagtcgtgct gcgccagggg cgctggcatg cgatggagca
    23041 gactgtgacc acctgcgaag ggcaaaccat taaacctgat ttagattcgc aggcgatagc
    23101 gcatatcgag cgccgccaga gccgctcttc tgttgatgtc agcgtggcat ggctggaagc
    23161 gcccgaaggc tcgcaattac tgttggtggc aaactctgat ttctgtcgct ggcaacccaa
    23221 cgagaaaacg ttctgattta ccagtggccc atacccatat gaccgccacc accgcagccg
    23281 ccgtaaccca tgcccattcc ggtaccgcgc ggaatacccg cttcagccat cgcaatatct
    23341 cgtttcaccc gtaactcatc taacgactga cgcaaattct ccatctcttt ggcgaccgcg
    23401 ttaattttgc tgctatccgg tgggttcgcg gctaacaggg cattgtattc ataacgcttt
    23461 gtcaccagtt gctgttgcag tgcgctgctt tgagcgtaaa agtcattatg gattttctgc
    23521 cacgctgtct gttgttcgct ggtcaaaggc gcggcatttt gctgccacat accgtgtccg
    23581 ccgtgagcaa atgccgatgt cgatcccatc gccattgctg aaagcgccat cattaccagg
    23641 gcaattttcg tgttccgttt catggttaat cctccagtgg ttgtcttact tcgggtattg
    23701 catctttcgt gccaacggcg aaacgctgat atgacgggta atctggcatg ataaacgagt
    23761 aaaaatgact cgcctgttgt gggtagcgag tcatttttac tcattgaaac ttaagccttt
    23821 gtgttacagc gcagggtaag cgctgataaa agatggcatg atttctgctg tcagaaaggg
    23881 atgagcaggc aaagaagaag atgcgtttta tgcaacgttc taaagactcc ttagctaaat
    23941 ggttaagcgc gatcctcccc gtggtcattg ttgggctggt ggggctgttt gcggtgacag
    24001 tgattcgcga ttatgggcgc gagactgccg ccgccagaca aacgctgctg gaaaaaggca
    24061 gtgtacttat ccgtgctctc gaatccggct cgcgcgtcgg catggggatg cgcatgcatc
    24121 atgcgcagca gcaggcatta ctggaagaaa tggccgggca gcctggtgta cgttggtttg
    24181 cggtcacgga tgaacaagga acaatcgtga tgcatagcaa ctccggcatg atgggaaaac
    24241 agctttattc cccgcaggaa atgcagcagt tacatccggg agatgaagaa gcgtggcggc
    24301 ggatcgatag cgcagacggc gagcctgttc tggaaattta tcgccagttt caaccgatgt
    24361 ttgctgctgg aatgcaccgg atgcgccata tgcagcagta tgccgcgaca ccacaagcaa
    24421 tttttatcgc tttcgacgcc agtaacattg tgagtgctga agatcgtgag cagagaaaca
    24481 ccctgattat cctcttcgcc ctggcgacgg tcttgctggc aagcgtgttg tcattcttct
    24541 ggtatcgccg atatctgcgc tcgcggcagc tattgcagga tgaaatgaag cgcaaagaga
    24601 agctggtggc actggggcat ctggcggcag gcgttgccca cgaaatccgt aacccacttt
    24661 cctcgattaa agggctggcg aaatactttg ccgaacgcgc gtcagcaggg ggagaagcgc
    24721 atcaactggc gcaggtgatg gcgaaagaag ccgaccgttt aaaccgcgtg gtaagcgagt
    24781 tgctggaact ggttaagcca acgcatctgg ctttgcaggc ggtggagctc aacacgctga
    24841 ttaaccactc attacagctg gtaagccagg atgcaaacag ccgggagatc cagttacgct
    24901 ttaccgccaa cgacacatta ccggaaattc aggccgatcc ggacaggctg actcaggtcc
    24961 tgttgaatct ctatctcaat gctattcagg cgattggtca gcatggcgtg attagcgtga
    25021 cggccagcga aagcggcgcg ggcgtgaaaa tcagcgttac cgacagcggt aagggaattg
    25081 cggcagatca gcttgaagcc atcttcaccc cgtacttcac caccaaagcc gaaggtaccg
    25141 gactggggct ggcggtcgtg cataatattg ttgaacaaca cggtggtaca attcaggtcg
    25201 caagccagga gggaaaaggc gcaacgttca ccctctggct tccggtcaat attacgcgta
    25261 aggacccaca aggatgacgc acgataatat cgatattctg gtggtggatg atgacattag
    25321 ccactgcact attttgcagg ctttgctgcg cggctggggc tataacgtcg cgctggcgaa
    25381 cagcgggcga caggcgctgg agcaggtgcg ggaacaggtt tttgatcttg tgctttgcga
    25441 tgtgcgaatg gcagagatgg acggcatcgc cacgctcaaa gagatcaaag cgttaaatcc
    25501 ggcaattccg gtactgatta tgaccgcgta ctccagcgtc gagacagcgg tagaggcgtt
    25561 gaaaaccggg gcgctggatt atctcatcaa gccgctggat ttcgataacc tgcaggcgac
    25621 gctggaaaag gcgctcgcac atacgcacag tgttgacgct gaaacgcctg cggtgtctgc
    25681 cagccagttc agtatggtcg gtaaaagccc ggcgatgcaa cacctgctca gtgaaatcgc
    25741 cctcgtcgcg ccatcggaag ccacggtgct gatccacggc gattccggca ccggtaaaga
    25801 gctggtcgcc agggcgattc acgccagtag cgcacgtagc gaaaaaccgc tggtaacgct
    25861 caactgcgcg gcgctcaacg aatccttgct ggagtctgaa ttgttcggtc acgaaaaagg
    25921 ggcgtttacc ggggccgaca aacgccggga agggcgcttt gttgaggcgg acggcggcac
    25981 gttgtttctc gatgaaattg gcgatatctc gccgatgatg caggtgcgtc tgctacgtgc
    26041 gattcaggag cgcgaagttc agcgtgtcgg cagcaaccag actatttcgg ttgatgtccg
    26101 gctgattgcg gcgacccatc gcgatcttgc cgcagaggtg aatgccgggc gttttcgcca
    26161 ggatctctac tatcggctga acgtggtggc gattgaagta ccatcgctgc ggcaacggcg
    26221 ggaagatatt cctctgctgg ctggccattt tcttcagcgc tttgccgaac gtaatcgcaa
    26281 ggcggtaaaa ggctttacgc cccaggcgat ggatctgctg attcattacg actggccggg
    26341 aaatattcgt gaactggaaa acgcggtgga acgggcggtg gtgctgctga ccggggaata
    26401 tatttccgaa cgcgagctgc cgctggcgat tgccagtacg ccgatcccgc tggtacaaag
    26461 tcaggatatt cagccgctgg tggaagtcga aaaagaagtg attctggcgg cactggagaa
    26521 aacgggcggc aacaaaaccg aagccgcccg tcagttaggg atcacgcgta aaacgctatt
    26581 ggcaaaactg tcgcgttagt tctgctcgcg ttcgatagcg cgccagccga tatctttccg
    26641 gcagaagcag tcgtcccaat ggatatcggt cattaaggca taagcgcgtt tctgtgcttc
    26701 tgccacggta tgacccagcg cggtaacgca cagtacgcgc ccgccgctgg tcactacctg
    26761 ctcgtcatcc gccagtttcg tgcccgcgtg gaacactttg ccgtctgcca cttcttccag
    26821 cggcaggccg tggatcacat cgccggtgcg gtaatcgccc ggatatccac ccgcagccat
    26881 caccacgccg agagaagcgc gttcatccca ctcggacgtt ttctcgtcca gcttgccttc
    26941 acaggccgcc aggcagagct caaccagatc ggacttcatg cgcagcataa tcggctgggt
    27001 ttccggatcg ccaaagcggc agttaaattc aataaccttc ggattgccct gtttgtcgat
    27061 catcaggccc gcgtagagaa aaccagtgta ggtgttgcct tccgccgcca tgcctttcac
    27121 ggttggccag atgatacgtt ccatcgtgcg ctgatgaacc tcatcggtca ctactggcgc
    27181 aggggagtaa gcgcccatcc cgccggtgtt cggcccggta tctttatcgc ctacgcgttt
    27241 gtgatcctgg ctggtggcca tcggcagcac atgctcgccg tccaccatca cgataaagct
    27301 cgcttcttcg ccgtcgagga actcttcgat aacaatgcga tgacccgcgt cgccaaacgc
    27361 gttgcccgcc agcatgtcgc gcacggcggc ttcagcttct tccagcgtca tcgccacgat
    27421 aacgcctttc ccggcggcca gaccgtccgc tttaatgacg attggcgcgc ctttctcacg
    27481 cagatacgcc agcgcaggtt ctacctcggt gaagttctgg tattccgctg tgggaatgtt
    27541 atggcgggcc agaaagtctt tggtgaatgc tttagaacct tccagttgtg cagcacctgc
    27601 ggttgggccg aagattttca gcccggcggc gcggaaggta tcgactacgc ctttcaccag
    27661 cggcgcttcc gggccgacga tggttagatc taccttttcg ttttgtgcga aatccagcag
    27721 cgccgggata tcggttacgc cgatagcgac gttctgtagc gcgggttcca gcgcagtgcc
    27781 tgcattaccc ggagcaacaa aaacagtttc aaccagcggc gactgagccg ctttccaggc
    27841 cagcgcgtgc tcgcgcccgc cgttaccaat cactaatact ttcatctatt gctccattaa
    27901 tggcggaagt ggcgcatgtc ggtgaagagc atcgcaatac cgtgctcgtc ggcggcggca
    27961 atcacttcgt catcacggat agaaccgcca ggctggatta cacaggtcac acccgcagcg
    28021 gcggcggcat caataccgtc gcggaacggg aagaatgcgt cagaagccat cgaggaacct
    28081 ttcacttcca ggccttcatc ggcagcttta ataccggcga ttttcgcgga gtacacgcgg
    28141 ctcatctggc ctgcgccaat accgatggtc atattgtttt tggcatagac gatagcgttg
    28201 gatttcacga acttcgccac tttccagcag aacagcgcat cacgcagttc ctgttcgctc
    28261 ggctggcgtt tggtgacgac gcgcagttct tccgcaccga ccattcccag atcgcgatcc
    28321 tgaaccagca gaccgccgtt cacgcgtttg aaatcaagac ccggaacacg ctcgccccac
    28381 tgaccgcagg tcaggacgcg tacgttctgt ttggcggcag tgattttcag ggcttcttcg
    28441 ctggcggacg gcgcaataat cacttcaaca aactgacgag aaatgatggc ctgtgcggtt
    28501 tccgcatcca gctcgcgatt aaaggcaatg atgccgccga atgcggaggt cgggtcggtt
    28561 ttgtacgcgc gatcgtaagc atcaagaatg gaattgccga tagccacgcc gcaagggttg
    28621 gcgtgcttca caatcacaca tgccggctcg gcgaactctt tcacgcactc cagcgccgca
    28681 tcggtatcgg cgatgttgtt ataagagagg gctttaccct gaacctgggt tgcggtagca
    28741 acggaggctt ctttgacatt ctcttctata tagaaggcag cctgctggtg gctgttctcg
    28801 ccgtaacgca tatcctgctt cttaatgaag ttcaggttca gcgtgcgtgg gaagcgaccg
    28861 gcggcttctt tgctttcacc gtgataggct gggaccatgc tgccgaagta gttggcaatc
    28921 atgctgtcgt aggcggcagt gtgttcgaag gctttgatgg caaggtcgaa acgggttgca
    28981 agcatcagcg atccttcgtt ggcatccatc tctttaataa tggcgtcgta gtcgctgctc
    29041 ttcaccacga ttgcgacatc tttatggttc ttggcggcgg agcgcaccat cgttgggccg
    29101 ccgatatcga tgttctctac cgcatcttcc agcgagcagc cttcacgagc aacggtctgg
    29161 gcgaacggat acaggttaac aaccaccata tcgataggca ggatctgatg ttcttccata
    29221 atggcatcgt ccaggccgcg acggcccaga atgccaccat gtactttcgg atgcagggtc
    29281 ttcacgcgtc catccatcat ctccgggaaa ccggtgtaat cggaaacttc ggttaccggc
    29341 agaccttttt ctgctaacag acgggctgtg ccccctgtag acagcagctc cacaccgcgt
    29401 gcggaaagtg cctgggcgaa ttcgacgata ccggctttgt cagaaacact gagcagagcg
    29461 cggcggactg gacgacgttg ttgcatggta aatcccctgg atttgactat tacagagagc
    29521 gttagctgaa tttttcgcga aaaactcagc taacgcccct aacggggcat ccttattttt
    29581 cgcccgcatt gtaacgaaaa cgtttgcgca acgctcgcga atttttctct ttcaatggtg
    29641 atcacaattt tgacagtgtt taccgtggga aaaatacaga aattacattg atgattgtgg
    29701 ataactctgt gcgtaaaaag gtataaagtg gggttttgct ggggaatgca gcagtcagtc
    29761 atttttatgc aatttttcta ttgcggcctg cggagaactc cctataatgc gcctccatcg
    29821 acacgacgga tgtgaatcac ttcacacaaa cagc
//
LOCUS       DAZFJL010000049        29799 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFJL010000049 DAZFJL010000000
VERSION     DAZFJL010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.049"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..480
                     /locus_tag="ACHJ8C_002114"
     CDS             <1..480
                     /locus_tag="ACHJ8C_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO5551971.1"
                     /translation="GQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAA
                     YLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAF
                     TLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSNHPKLQGIAQQNSFKHT"
     gene            636..2171
                     /gene="gadC"
                     /locus_tag="ACHJ8C_002115"
     CDS             636..2171
                     /gene="gadC"
                     /locus_tag="ACHJ8C_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO5551972.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDAKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKSKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2302..3621
                     /gene="digH"
                     /locus_tag="ACHJ8C_002116"
     CDS             2302..3621
                     /gene="digH"
                     /locus_tag="ACHJ8C_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO5551973.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPAMTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDMLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3999..4163
                     /locus_tag="ACHJ8C_002117"
                     /pseudo
     CDS             3999..4163
                     /locus_tag="ACHJ8C_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            4188..6587
                     /gene="dosP"
                     /locus_tag="ACHJ8C_002118"
                     /gene_synonym="pdeO"
     CDS             4188..6587
                     /gene="dosP"
                     /locus_tag="ACHJ8C_002118"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO5551974.1"
                     /translation="MKLTDADTAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPQYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCINEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGASFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWYDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNINIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGIGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGIETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(6777..7208)
                     /gene="osmC"
                     /locus_tag="ACHJ8C_002119"
     CDS             complement(6777..7208)
                     /gene="osmC"
                     /locus_tag="ACHJ8C_002119"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO5551975.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDVIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            7553..7768
                     /gene="bdm"
                     /locus_tag="ACHJ8C_002120"
     CDS             7553..7768
                     /gene="bdm"
                     /locus_tag="ACHJ8C_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO5551976.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            7870..8007
                     /gene="sra"
                     /locus_tag="ACHJ8C_002121"
     CDS             7870..8007
                     /gene="sra"
                     /locus_tag="ACHJ8C_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO5551977.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            8164..9861
                     /gene="maeA"
                     /locus_tag="ACHJ8C_002122"
     CDS             8164..9861
                     /gene="maeA"
                     /locus_tag="ACHJ8C_002122"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO5551978.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDYRRTSI"
     gene            9995..11005
                     /gene="adhP"
                     /locus_tag="ACHJ8C_002123"
     CDS             9995..11005
                     /gene="adhP"
                     /locus_tag="ACHJ8C_002123"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO5551979.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSASASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     ARIVQEKAGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            11191..11469
                     /locus_tag="ACHJ8C_002124"
     CDS             11191..11469
                     /locus_tag="ACHJ8C_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5551980.1"
                     /translation="MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLA
                     DIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDAYILDYEDYH"
     gene            11469..11753
                     /gene="yddM"
                     /locus_tag="ACHJ8C_002125"
     CDS             11469..11753
                     /gene="yddM"
                     /locus_tag="ACHJ8C_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO5551981.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(11982..12635)
                     /gene="fdnI"
                     /locus_tag="ACHJ8C_002126"
     CDS             complement(11982..12635)
                     /gene="fdnI"
                     /locus_tag="ACHJ8C_002126"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO5551982.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPIQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGL"
     gene            complement(12628..13512)
                     /gene="fdnH"
                     /locus_tag="ACHJ8C_002127"
     CDS             complement(12628..13512)
                     /gene="fdnH"
                     /locus_tag="ACHJ8C_002127"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO5551983.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEGNHE"
     gene            complement(13525..16572)
                     /gene="fdnG"
                     /locus_tag="ACHJ8C_002128"
     CDS             complement(13525..16572)
                     /gene="fdnG"
                     /locus_tag="ACHJ8C_002128"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15985..15987),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO5551984.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDDTLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKSTLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGIANGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            16805..17686
                     /gene="yddG"
                     /locus_tag="ACHJ8C_002129"
     CDS             16805..17686
                     /gene="yddG"
                     /locus_tag="ACHJ8C_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO5551985.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDQIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            17959..19347
                     /gene="narU"
                     /locus_tag="ACHJ8C_002130"
     CDS             17959..19347
                     /gene="narU"
                     /locus_tag="ACHJ8C_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO5551986.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            19442..23182
                     /gene="narZ"
                     /locus_tag="ACHJ8C_002131"
     CDS             19442..23182
                     /gene="narZ"
                     /locus_tag="ACHJ8C_002131"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO5551987.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMISYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDTVAGVAFPYFGGIENPHFRSVKHDPV
                     LVRQLPVKNLTLADGTVCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVT
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLSLKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDNGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGPTEGIQPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            23179..24723
                     /gene="narH"
                     /locus_tag="ACHJ8C_002132"
     CDS             23179..24723
                     /gene="narH"
                     /locus_tag="ACHJ8C_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO5551988.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQDEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYECQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            24723..25418
                     /gene="narW"
                     /locus_tag="ACHJ8C_002133"
     CDS             24723..25418
                     /gene="narW"
                     /locus_tag="ACHJ8C_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO5551989.1"
                     /translation="MQILKVLGLLMEYPDELLWECKDDALALIRRDAPMLTDFALDLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLNCRE
                     LPDYLPLYLEYLSVIPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     PLTSDSVTKQIRSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            25415..26095
                     /gene="narI"
                     /locus_tag="ACHJ8C_002134"
     CDS             25415..26095
                     /gene="narI"
                     /locus_tag="ACHJ8C_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO5551990.1"
                     /translation="MIQYLNVFFYDIYPYLCGTVFILGSWLRYDYGQYTWRAASSQML
                     DKRGMVLWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLT
                     LIGGAGLLVRRLTNPRIRATSSGADILILAILLIQCILGLTTIPFSAQHPDGSEMMKL
                     VGWAQSIVTFQGGASTHLDGVAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            26175..27068
                     /gene="yddE"
                     /locus_tag="ACHJ8C_002135"
     CDS             26175..27068
                     /gene="yddE"
                     /locus_tag="ACHJ8C_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO5551991.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKQNDDYRISLEQGTPGFEPPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALTAISKQIGCNGFFPFQIRPGKSETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLSHDGNVLRVKGHQGRALGRDGVIDVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIDF"
     gene            complement(27164..28009)
                     /gene="nhoA"
                     /locus_tag="ACHJ8C_002136"
     CDS             complement(27164..28009)
                     /gene="nhoA"
                     /locus_tag="ACHJ8C_002136"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO5551992.1"
                     /translation="MTPILNHYFARINWLGAAAANIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEDKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLMPDIMQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSALWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            28184..28753
                     /gene="yddH"
                     /locus_tag="ACHJ8C_002137"
     CDS             28184..28753
                     /gene="yddH"
                     /locus_tag="ACHJ8C_002137"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO5551993.1"
                     /translation="MSQFIPVELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVRGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(28757..28984)
                     /gene="pptA"
                     /locus_tag="ACHJ8C_002138"
     CDS             complement(28757..28984)
                     /gene="pptA"
                     /locus_tag="ACHJ8C_002138"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFO5551994.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADINDVIIRHLNSKDSSISISL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(29287..29493)
                     /locus_tag="ACHJ8C_002139"
     CDS             complement(29287..29493)
                     /locus_tag="ACHJ8C_002139"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO5551995.1"
                     /translation="MGQLMAKETADSRTEYVYDAADLLLEIRCRWSDARETDAPEIIC
                     FSYDRLGQLLTEETVQKFKIIHLT"
     gene            29508..29708
                     /locus_tag="ACHJ8C_002140"
     CDS             29508..29708
                     /locus_tag="ACHJ8C_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
                     /protein_id="HFO5551996.1"
                     /translation="MLKISAKWLREAGFEIGTGVTVKISKGCLILLADNNEVQELRRE
                     LYQVKQAIKGMRDGMFSVLNES"
BASE COUNT         7239 a         7663 c         7536 g         7361 t
ORIGIN      
        1 ggtcagattg gtactttcgc catcaacttc tcccgcccgg cgggtcaggt gattgcacag
       61 tactatgaat tcctgcgcct cggtcgtgaa ggctatacca aagtacagaa cgcttcctac
      121 caggttgctg cttatctggc ggatgaaatc gccaaactgg ggccgtatga gttcatctgt
      181 acgggtcgcc cggacgaagg catcccggcg gtttgcttca aactgaaaga tggtgaagat
      241 ccgggataca ccctgtatga cctctctgaa cgtctgcgtc tgcgcggctg gcaggttccg
      301 gctttcactc tcggcggtga agccaccgac atcgtggtga tgcgcattat gtgtcgtcgc
      361 ggcttcgaaa tggactttgc tgaactgttg ctggaagact acaaagcctc cctgaaatat
      421 ctcagcaacc acccgaaact gcagggtatt gcccagcaga acagctttaa acacacctga
      481 taacgtttaa cggtaacggt gtcccgaaac gaagccgttt cgggacaatt tccaaagtct
      541 gttcactggc attagcaacg gaaaatattg ttctgaatac gcttcagaac aaaacaggtg
      601 cggttccgac aggaataccg ttttaggggg ataatatggc tacatcagta cagacaggta
      661 aagctaagca gctcacatta ctcggatttt ttgccataac ggcatcgatg gtaatggctg
      721 tttatgaata ccctaccttc gcaacatcgg gcttttcatt agtcttcttc ctgctattag
      781 gcgggatttt atggtttatt cccgtgggac tttgtgctgc ggaaatggct accgtcgacg
      841 gctgggaaga aggtggcgtc ttcgcctggg tatcaaatac tctggggccg agatggggat
      901 ttgcagcgat ctcatttggc tatctgcaaa tcgccattgg ttttattccg atgctctatt
      961 tcgtgttagg ggcactctcc tacatcctga aatggccagc tctgaatgaa gaccccatta
     1021 ccaaaactat tgcagcactc atcattcttt gggcgctggc attaacgcag tttggtggca
     1081 cgaaatacac ggcgcgaatt gctaaagttg gcttcttcgc cggtatcctg ttacctgcat
     1141 ttattttgat cgcattagcg gctatttatc tgcactccgg tgcccccgtt gctatcgaaa
     1201 tggatgcgaa gaccttcttc cctgacttct ctaaagtggg caccctggtt gtatttgttg
     1261 ccttcatttt gagttatatg ggcgttgaag cttccgcaac ccacgtcaat gaaatgagca
     1321 accccgggcg cgactatcca ctggctatgt tactgctgat ggtggcggca atctgcttaa
     1381 gctctgttgg cggtttgtct attgcgatgg tcattccggg taatgaaatc aacctctccg
     1441 caggggtaat gcaaaccttt accgttctga tgtcacatgt ggcaccggaa attgagtgga
     1501 cggttcgcgt gatctccgca ctgctgttgc tgggtgttct ggcggaaatc gcttcctgga
     1561 ttgttggtcc ttctcgcggg atgtatgtca cagcgcagaa aaacctgctg ccagcggcat
     1621 tcgctaaaat gaacaaaaat ggcgtaccgg taacgctggt catttcgcag ctggtgatta
     1681 cttctatcgc gttgatcatc ctcaccaata ccggtggcgg taacaacatg tccttcctga
     1741 tcgcactggc gctgacggtg gtgatttatc tgtgtgctta tttcatgctg tttattggct
     1801 acattgtgtt ggttcttaaa catcctgact taaaacgcac atttaatatc cctggtggta
     1861 aaggggtgaa actggtcgtg gctattgtcg gtctgctgac ttcaattatg gcgtttattg
     1921 tttccttcct gccgccggat aacatccagg gtgactctac cgatatgtat gttgaattac
     1981 tggtcgttag tttcctggtg gtacttgccc tgccctttat tctctatgct gttcatgatc
     2041 gtaaaagcaa agcgaatacc ggcgtcactc tggagccaat caacagtcag aacgcaccaa
     2101 aaggtcactt cttcctgcac ccgcgtgcac gttcaccaca ctatattgtg atgaatgaca
     2161 agaaacacta accatgtctg aatggttata agacaaggga gcgataattc atcgctccct
     2221 ttttcgtgct tgttgtcgta ttgacttaac cgggataagt acgagaatga gcgcacatct
     2281 gtttaccgga aaccagcaca tatggatatc tgctcccgaa acgagaaatt agcgattaga
     2341 agaccagcga tactggttgc actggcactt ttactgtgta gttgtaaaag cacgcctcca
     2401 gagtccatgg tgacaccacc agcgggttca aagccaccag ccatgacgca acaatcgtca
     2461 caaccgatgc gtggcatctg gctggccacg gtgtctcgcc tcgactggcc accggtttcc
     2521 tcggttaaca ttagtaaccc caccagccgg gcacgtgtac aacaacaggc gatgatcgac
     2581 aaactggatc atctgcaacg tctcggcata aacacggtct ttttccaggt caagccggat
     2641 ggtaccgccc tgtggccatc gaaaattttg ccgtggtccg atcttatgac cggtaagatt
     2701 ggtgaaaatc cgggttacga tccgctgcaa tttatgctcg acgaagccca caagcgtggg
     2761 atgaaagtac acgcctggtt taacccctat cgcgtatcgg ttaatacgaa gcccggtact
     2821 atcagggaac tgaatagcac cctgtctcaa caaccggcca gcgtctatgt gcaacaccgc
     2881 gactggatca gaacgtccgg cgatcgcttt gtcctcgacc cgggcatccc tgaggttcag
     2941 gactggatca catcaatagt cgcggaagtg gtttcccgct atccagtaga tggcgtgcag
     3001 tttgatgact atttctatac tgagtcacca ggttcacggc taaatgataa cgaaacgtac
     3061 cgcaaatacg gcggcgcatt tgcgtcaaaa gcagactggc ggcgcaacaa tactcagcag
     3121 ttaattgcga aggtatcgca caccattaaa agcattaagc cggaagtcga atttggcgtt
     3181 agcccggcag gcgtgtggcg taaccgatca cacgatccgc tcggttccga tacccgaggc
     3241 gcggcagcct atgacgaatc ctacgctgat acccgtcgat gggtggaaca agggttgctg
     3301 gattacattg ctccccaaat ttactggcca ttctcacgga gtgccgcgcg ttatgacatg
     3361 ttggcaaaat ggtgggcgga tgtcgttaaa ccgaccagga cccgcctgta tatcggtatc
     3421 gccttctata aagtgggtga accttcaaag atagagccag actggatgat taacggcggc
     3481 gtaccggaac tgaaaaagca gctcgatctt aacgatgctg tgccggaaat tagcggcaca
     3541 atcttgttcc gtgaggacta tctgaataaa ccgcagacgc aacaagcggt cagctatctg
     3601 caaagtcgtt ggggcagtta aactcccatt cttactttgt aacaagccgg tgctttaccc
     3661 actggcttgt taccctcttg aactatccca ctagcaatca tgcttaaata cgtcaaatta
     3721 accacacaat taaccacact tagttaaaca gccataatcc ccctaataaa aatgtatgaa
     3781 tatgaaaaaa catacgatat tcgctgtcac cgaaaaataa ttacgatggg ctatactctt
     3841 ctgaagaaca gtagcgtaga ttgaaactgt cctaagtcgc ttatttcttt tcagtatatc
     3901 ttcatatttc aggagaatat ggtgcagtta gtcgatcccg cacgttgtgt ttggttgatt
     3961 ttggcctgcg gtttaaccat aaaggtcgac attattttaa agccgcaaac ggcgaggata
     4021 ttgccctctc actttccatc ggtgccgcca tgtttaatgg tcatcctgac tatgagcgcc
     4081 tcattcaaat agccgatgaa gctctgtata tcgccaaaag acgaggtaga aaccgtgttg
     4141 aactctgtaa agccagtctt tagatgcgcc aggatgcaga ggtaatcatg aagctaaccg
     4201 atgcggatac tgccgccgat ggcatttttt tccccgccct tgagcaaaat atgatgggcg
     4261 cggtgttaat taacgaaaat gatgaagtga tgtttttcaa ccccgccgca gagaagctct
     4321 gggggtacaa acgtgaagaa gtcattggca ataacattga tatgctgatt ccgcgggatt
     4381 tgcgtcctgc gcatcctcaa tacattcgtc acaaccgtga aggtggtaaa gcgcgcgtcg
     4441 aggggatgag tcgggagctg cagctggaga aaaaagacgg cagtaaaatc tggacccgtt
     4501 ttgcgctatc gaaagtgagc gccgagggga aagtttatta cctggcgctg gtacgggatg
     4561 ccagcgtaga aatggcgcaa aaagaacaga cccgacaatt gattattgcc gttgaccatc
     4621 tcgaccgacc ggtgattgtc ctcgatccgg aacgccatat tgtgcagtgc aatcgcgcat
     4681 ttaccgaaat gtttggttac tgcattaacg aagccagcgg tatgcagccc gatacactcc
     4741 tgaacattcc agaattccct gctgataacc gtattcgttt acaacagttg ctatggaaaa
     4801 ccgcccgcga tcaggacgaa tttctgctgt tgacgcgcac cggtgaaaaa atctggatta
     4861 aagcctctat cagcccggtt tatgacgtgc tcgcgcatct gcagaacctg gtaatgacat
     4921 tctcggatat caccgaagaa aggcaaatcc gccagcttga aggcaatatt ctcgccgcca
     4981 tgtgcagcag ccccccattt catgaaatgg gggaaatcat ttgtcgtaac atcgaatctg
     5041 ttctcaacga atcgcatgtt tcgctgttcg cgctgcgcaa cgggatgccg atacactggg
     5101 cgtcatcttc gcacggtgca gaagttcaaa atgcgcaaag ctggtcagcg accattcgtc
     5161 agcgcgatgg cgcgcctgcg gggatcctgc aaattaaaac ctcgtcagga gcagaaacca
     5221 gcgcctttat cgaacgcgtg gcagatatca gccagcatat ggccgcgctg gcgctggaac
     5281 aggaaaaaag ccgtcagcat attgaacaac tcatccaatt tgatccaatg accggtctgc
     5341 caaatcgcaa taacctgcac aattacctcg atgacctggt cgacaaagcc gtctctccgg
     5401 tggtgtatct catcggtgtt gaccatattc aggatgtgat tgatagcctt ggctatgcgt
     5461 gggccgatca ggcattgctg gaagtggtca atcgctttcg tgaaaaactc aaaccggatc
     5521 agtatctctg tcgtattgaa ggtgcgtctt ttgtcctcgt gagcctggaa aacgatgtca
     5581 gtaacattac ccagatcgcc gatgagctac ggaatgtggt cagcaagccg ataatgattg
     5641 acgataaacc attcccgctc accttgagca ttggcatcag ctacgacgtg ggtaaaaacc
     5701 gcgattacct gctctccact gctcacaatg caatggattt tattcgcaaa aatggcggta
     5761 acggctggca gttcttcagc ccggcgatga acgaaatggt aaaagagcgt ttggttttag
     5821 gcgcagcgct aaaagaagcg attagcaata accaacttaa actggtttat cagccgcaaa
     5881 tcttcgcaga aacgggtgaa ctgtacggca tcgaagccct tgctcgctgg tacgatcccc
     5941 tgcatggtca tgtgccccct tcacggttta ttcctctcgc agaagagatt ggtgaaatcg
     6001 aaaatattgg gcgctgggtc atcgcggaag cttgccgtca gttagcagaa tggcgtagcc
     6061 agaatattaa tatcccggcg ttatccgtta acttgtctgc gctgcacttt cgcagtaatc
     6121 agctgcctaa tcaggtatct gatgcaatgc acgcctgggg tattgacggc caccagctga
     6181 cggtggaaat cacggaaagc atgatgatgg aacacgatac cgaaatcttt aagcgcattc
     6241 agatcctgcg cgatatgggc atcggtttat cggtagatga ttttggtaca ggcttttccg
     6301 gattatcccg cttagtcagt cttccggtaa cggaaatcaa aattgataaa agttttgtcg
     6361 atcgttgtct gaccgaaaaa cgcatccttg ccttactcga agccattacc agcattgggc
     6421 aaagcctcaa tttaaccgtc gtggctgaag gcatcgaaac caaagagcaa tttgagatgc
     6481 tacgcaagat ccactgtcgc gttattcagg gatatttctt ttcccgcccc ctgcccgccg
     6541 aagaaattcc aggttggatg agcagcgtgt taccgctgaa aatctgacaa attcctctcg
     6601 cccgcactcg cgggttttta ttcaacgtga caccgtcact taaatactat gatttccgct
     6661 acgtttagtt ggacaacgac aacgcatagt catgtcagaa caatcccttc tcagcctgat
     6721 gtgattgtgc tgacgcatca ggcattcacg cgcctgacgc gtcatccggc aacgctttac
     6781 gatttcaact ggtaatccag cgtaatttcc gctttcagta cctgagaaac cgggcatcct
     6841 gcttttgctt tctggataat gacgtcaaag gtggaggcat caatacccgg caccgcaact
     6901 tcgctcttca gtgcgatttt agtaatcgca aaaccggcat ccactttatc cagcgagaca
     6961 tcggcggtgg tatcaatcga tgttggcgtg aatcccgctt cccccagcat taatgaaagc
     7021 gccattgaga aacatgcggc atgcgctgcg ccaatcagtt cttcagggtt ggttcctttt
     7081 tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt tcagcacgcc gctctcagta
     7141 gatactgttc ccttcccgcg tttgatatcg ccttcccagt gtgcctgacc tttcttatgg
     7201 attgtcattg ttgctctcct gtgggcttaa aaaggaaatt atagactacg tgagaaacag
     7261 cacgaggata agcaggataa tccgaataaa atccggcgaa aatataattc ctgcacttaa
     7321 attaaattcc cataatagat aaccggaaat atataactca agagatattg catcaatatc
     7381 aatcaactaa tcacaatgat aatacctatc aatatcatgg agttaaaata taaaaatcat
     7441 caaccaggac taatcttaac aacgaagcgg caaatatttg ccatgctgaa tgtgcgttat
     7501 aaatctgcga tccgtagcag acaccataaa tacacagaca cggagaatca ctatgtttac
     7561 ttattatcag gcagaaaatt caacagcaga acccgctctg gttaatgcca ttgagcaagg
     7621 ccttcgggca gagcacggtg ttgtcactga agatgacatt ctcatggagc tgaccaaatg
     7681 ggtggaagct tctgataacg acatcctcag tgatatctac cagcaaacga tcaattatgt
     7741 ggtcagtggt cagcacccta cgctttaagg tgctatgctt gatcggcaac ctaatttagg
     7801 ggtttagcac gtgtttcttc gctacggcga tgttgtcctt aaaactagca acaggattga
     7861 ggagttaaaa tgaaatcgaa ccgtcaggca cgtcatattc ttggactgga ccataaaatt
     7921 tctaaccagc gcaaaatagt taccgaaggt gacaaatcca gcgtggtaaa taatcccacc
     7981 ggcagaaaac gccccgctga aaagtaattc ataaccatca gtcctcaatg acgattaaac
     8041 accattgcct gcgcaatggt gtttttgttt ttatctgctt tatacttggt acttgacgcc
     8101 ctggcggtaa tgcaaagacg ataaaagccc ccagggatgg atattcaaaa aagagtgagt
     8161 gacatggaac caaaaacaaa aaaacagcgt tcgctttata tcccttacgc tggccctgta
     8221 ttgctggaat ttccgttgtt gaataaaggc agcgccttca gcatggaaga acgccgtaac
     8281 ttcaacctgc tggggttact gccggaagtg gtcgaaacca tcgaagaaca agcggaacga
     8341 gcatggatcc agtatcaggg attcaaaacc gaaatcgaca aacacatcta cctgcgtaac
     8401 atccaggaca ctaacgaaac cctcttctac cgtctggtaa acaatcatct tgatgagatg
     8461 atgccagtca tttacacccc aaccgtcggc gcagcctgtg agcgtttttc tgagatctac
     8521 cgccgttcac gcggtgtgtt tatctcttac cagaaccgcc acaatatgga cgatattctg
     8581 caaaacgtac caaaccataa tatcaaagtg attgtggtga ctgacggcga acgtattctg
     8641 gggcttggtg accagggcat cggcgggatg ggcattccga tcggtaaact gtcgctctat
     8701 accgcctgtg gcggcatcag tccggcctat acccttccgg tggtgctgga tgtcggcacc
     8761 aacaaccaac agctacttaa cgatccgctg tatatgggct ggcgtaatcc gcgtatcact
     8821 gacgacgaat actatgaatt cgttgatgaa tttatccagg ctgtgaaaca acgctggccg
     8881 gacgtactgt tgcagtttga agacttcgca caaaaaaatg cgatgccgtt acttaaccgc
     8941 tatcgcaatg aaatttgttc ttttaacgat gacattcagg gcaccgcggc ggtaacagtc
     9001 ggcacactga tcgcagccag ccgcgcggca ggcggtcagt taagcgagaa aaaaatcgtc
     9061 ttccttggcg caggttcagc gggatgcggc attgccgaaa tgatcatcgc ccagacccag
     9121 cgcgaaggat taagcgagga agcggcgcgg cagaaagtct ttatggtcga tcgctttggc
     9181 ctgctgaccg acaagatgcc gaacctgctg cctttccaga ccaaactggt gcagaagcgc
     9241 gaaaatctca gtgactggga taccgacagc gatgtgctgt cactgctgga tgtggtgcgt
     9301 aatgtaaaac cagatattct gattggcgtc tcaggacaga ccgggctgtt tacggaagag
     9361 atcatccgtg agatgcataa acactgtccg cgtccgatcg tgatgccgct gtctaacccg
     9421 acttcacgcg tggaagccac accgcaggac attatcgcct ggactgaagg taacgcgctg
     9481 gtcgccactg gcagtccgtt taatccagtg gtatggaaag ataaaatcta ccctatagcc
     9541 cagtgcaaca acgcctttat tttcccgggc atcgggctgg gtgttattgc ttccggcgcg
     9601 tcacgtatca ccgatgagat gctgatgtcg gcaagtgaaa cgcttgctca gtattcgccg
     9661 ctggtcctga acggcgaagg tctggtacta cctgaactga aagatattca gaaagtctcc
     9721 cgcgcaattg cctttgcggt tggcaaaatg gcgcagcagc aaggcgtggc ggtgaaaacg
     9781 tctgccgaag ctctgcaaca ggccattgat gataatttct ggcacgccga ataccgcgac
     9841 taccgccgta cctccatcta agcctgcgcc cggtagtgaa ggctaccggg caattttcct
     9901 ctcccttttt cagatctcat ccatactggg tagtggcgaa taaatctcat ttgcctcacc
     9961 tgctatgcag aacatcatcc gaaaaggagg aactatgaag gctgcagttg ttacgaagga
    10021 tcatcatgtt gacgttacgg ataaaacact gcgctcactg aaacatggcg aagccctgct
    10081 gaaaatggag tgttgtggtg tatgtcatac cgatcttcat gttaagaatg gcgattttgg
    10141 tgacaaaacc ggcgtgattc tgggacacga aggtattggt gtggtggcag aagttggccc
    10201 tggtgttact tcattaaaac caggcgatcg tgccagcgtg gcgtggttct atgaaggatg
    10261 cggtcattgc gaatactgta acagtggtaa cgaaacgctc tgccgttcag ttaaaaatgc
    10321 cggatacagc gttgatggcg ggatggcgga agagtgcatc gtggtcgccg attacgcggt
    10381 taaagtgccg gatggcctgg actcggcgtc ggccagcagc attacctgtg cgggggtcac
    10441 cacctacaaa gccgttaagc tgtcaaaaat tcgtccaggg cagtggattg ctatctacgg
    10501 tcttggcggt ctgggtaacc tcgccctgca atacgcgaag aatgtcttta acgccaaagt
    10561 gatcgccatt gatgtcaatg atgaacagtt aaaactggca accgaaatgg gtgcagattt
    10621 agcgattaac tcacgcaccg aagacgccgc cagaattgtg caggagaaag ccggtggcgc
    10681 tcacgcagcg gtggtgacag cggtagctaa agctgcgttt aactcggcgg tggatgctgt
    10741 ccgtgcaggt ggtcgtgtcg tggctgtcgg tctgccgccg gagtctatga gcctggacat
    10801 cccacggctg gtgctggatg gtattgaagt ggtcggttcg ctggtcggca cgcgtcagga
    10861 tctaaccgaa gccttccagt ttgccgccga aggcaaagtg gtgccgaaag tcgccctgcg
    10921 tccgttagcg gacatcaaca ccatctttac cgagatggaa gaaggcaaaa tccgtggccg
    10981 tatggtgatt gatttccgtc gctaagaggt ctttgctgcg actgccatgt tcgggtcgca
    11041 gcctgttttg cgagacgctt tccgcaattt cactcttctt cttttcctcc cccatctttc
    11101 ccctcacaat tcgcgaaaac gtagcgaaat ttgttgcgct gtacggaaca atagtgatag
    11161 tgttcctttc gagggaacaa ggatgaagag atgataatga atttcagaca caagggattg
    11221 cgtgacttgt ttcttctcgg caaaacctcc ggcgttatac cgacgcaagt caaacgatta
    11281 cgccaccgac tcgctgtgat tgatgcagca tgctgtcttg ctgatatcga tatgcccggt
    11341 taccgactac atccgttaag cggcgatcgc gatggaattt gggcgatatc tgtctcgggc
    11401 aactggcgaa tcacatttga attcgtcaat ggcgatgcat acatactgga ttacgaggac
    11461 tatcactgat gaaaatggcc aatcatcccc gcccggggga cattattcag gaatcactgg
    11521 acgaacttaa tgtcagcctg cgcgagtttg ccagagcaat ggaaattgcg ccctcaacgg
    11581 caagccgatt gctgaccgga aaagcagctt tgacgccaga aatggcaata aaactttccg
    11641 tggtgatcgg cagttcgccg caaatgtggc tgaatctgca aaatgcctgg agtctggcag
    11701 aggcagaaaa aacagtggat gtgtcgcgac tgcgccgtct ggttacgcaa taaaaaaatg
    11761 gcaccttata aagcgccatt tgaaactgat gacaaacgca acattgcccg atgcgctacg
    11821 cttatcaggc ctacacgttc actgcaatct attggatttg aacggtttgt aggccggata
    11881 aggcgttcac gccgcatccg gcatgaacaa agtgtacgtt gtctacaata aaaaaatggc
    11941 gccttttaca gcgccatttt cagcttaaag ctcaaaagag attataaccc ttcttcactc
    12001 tcttttttcg cttctgcctt ctcgatttca cgataccagc gcggatggtg tttcttcgcc
    12061 cagcgacggc tcaccttccc ttcgatcatc cctttaatcg atcctttcac ccagaatgcc
    12121 atatacatat ggatcaggat ggcgtggatc aggatgatac ccgcagccgc atggatcagc
    12181 aggctataac ggacaacctg tattgggaag tactgcgcaa agtacggacg ccagataatc
    12241 accccggtca ccagcagcac gaaaatcatg ctcatgattg accagaacat catcttttgc
    12301 ccggcgttgt acttaccgac atccgccact ttgtgctcat tgccttttaa tacttcgaca
    12361 atgttcaaca gccacggaat atctttctta tccgggatgt tgtgatgcac aaaacgcaca
    12421 aacataaaca tcagcgcgac gaaaatcgca atgccgaaga acgggtgcaa aatgcgcccc
    12481 atctgcggcg taccgaaggt ttgcgtcagc cattgcagcg tcgggaagaa gaacgaaatc
    12541 ccggacagcg ccaccaggaa gaagcaaatc accacggtcc agtgacaggc gcgatcaata
    12601 aatttggtgc gcacaatcat tttcgactta ctcatgattt ccctcctcgt catcgtccac
    12661 ttccttattc gggccaatac cgatgtagtg gaaaatcaac ccggcaaaag tggcaataaa
    12721 gccagccgct gccagcggtt tcaacgcgcc tttccacagg cttaccgagg tatcgatctt
    12781 cggatctttc ggcagaccat gatacagctc cggctgatcg gcatgatgca gcacgtacat
    12841 aacgtgcgta ccaccgaccc cttccgggtt gtagacgcca gcatgttcgt aaccacgcgc
    12901 tttcagtttc gctacgcgct gttccgccag ctccagcatc tccttcttgg tgccgaagtg
    12961 gatagccccg gtcggacagg ttttcacaca agccggttcc tggccgacgc tgacgcgatc
    13021 gacgcagagc gtgcatttat atacccggtt atcctctttg ttgaggcgcg gaatattaaa
    13081 cggacacccg gcaatgcagt aaccacagcc gatgcagttt tccgactgga aatcgacaat
    13141 cccgttagcg tactgaatga ttgcaccagc agacgggcat gccttcaggc agcccggatc
    13201 ttcacagtgc atacagccgt ctttacggat cagccactcc agcttgccgt tctgttcggt
    13261 ttcgctaaag cgcatcaccg tccaggactt ggcgctcaga tcggcggggt tatcgtaaac
    13321 cccgacgcag tgtcccactt catcacggat gtcgttccac tccgaacacg ccacctgaca
    13381 ggctttacag ccgatacagg tggaaacgtc gataagtttc gcgacttctg ctttgtaatc
    13441 acgcacctga gaaggcggcg tgatggagtt agttgcggac cttttgataa tgtcctgcgt
    13501 ttccatagcc atctgttcgc ccccttacgc cttctcgatg ttgactaaga acgctttata
    13561 ttccggcgtt tgcgagtttg catcaccgac attcggcgtc agagtgttag cgatataacc
    13621 tttacgcgcg acaccctcaa agccccagtg gattggaata cccaccgttt caacctgctg
    13681 accgtttaca ttcagcggtt tcagacggcg cgttaccaca gccaccgcgc ggataaagcc
    13741 acgcttgctg gagacagtga cacgatcgcc attggcaatg cctttcgccg ccgccagcgt
    13801 ttcgctgatt tccacaaact gttccggctg agcaattgcg ttgagcaatg cgtgcttggt
    13861 ccaggtgtgg aagtgctccg tcagacgata ggtcgtaccc acatacggga actgctcttt
    13921 tttacccatc cgcagtgcgt cttgttcata cagacgaaca accgggttag acaccacgtt
    13981 cgggtgcaac gggttagtgc ccagcggcgt ttcaattggc tcgtagtgtt ccgggaacgg
    14041 accttccgcc attttgttga tggcaaacag gcgtcccatc ccttccggct gcatgataaa
    14101 cggcccggtt ggcgtacccg gtgcggcatt gccgaagtcc ggaatatcgt tacccgtcca
    14161 cttgctaccg ttccactgga tcagcatccg tttcggatcc cacggtttac cgttgatatc
    14221 cgccgaagca cggttgtaga gtacgcgacg gttgagcggc cacgcccagg cccatcccag
    14281 cgtattcccc agaccggacg ggtcagagtt atcgcggtta gccatctggt tgccctgctc
    14341 ggtccagcta ccggtgtaga tccagcaaga agatgcggtt gtaccgtcat cacgcagatg
    14401 cgcaaagcta ctcagcaact gacctttctt cgcaatcagc acgccattag cgtcatagag
    14461 atcttccagc gcatagccgt tgttctcttt cgccacttcg tcagactgcg gttcgtgcgg
    14521 ctgcttgtag ttccagctca tcttcatcag cggttctacg cctttaccac cctcggcctg
    14581 gtacagctcg cgcagatgat ggtagatacc cgccagaatt tcgccgtcgt tacgtgcttc
    14641 gcccggcgcg tcctgacctt tccagtgcca ctgcaaccag cgaccggagt tagcgataga
    14701 accatcttct tcagcaaagc aggtcgaagg cagacggaat acttcagtct gaatagatgc
    14761 cggatcgaca tcgttcgact cgccgtggtt ctgccagaag gtagaggttt cagtcaccag
    14821 cggatcgata accaccatgt acttcagctt gctcaggcag ctcaccactt tgtttttgtc
    14881 cgggaaggac gcaaccgggt taaagccctg gcagaaataa ccggtgactt taccttcatc
    14941 catcatgttg aaatacttga tgacgtcgta ggtctggtcc cacttcggca gccagtcgta
    15001 gccccagttg ttctctttct gcgcggcatc gccgtagaaa gatttcatca ggctaacgaa
    15061 aaacttcgga tagttgctcc agtagttcac ctgatcagcc agcgtcgatt tcggcgtgtt
    15121 cgcttccaga tacgactgca aatcaacctg tttttctgac ggcagcgtca gataacctgg
    15181 caggctggta gagagcaggc ctaagtcagt caggccctga atgttggagt gaccacgcaa
    15241 tgcgttcacg ccgccaccgg ccatacccat gttaccgagc agcaactgga tcatcgccat
    15301 cgtacggatg ttctgcgcac ccacggtgtg ctgcgtccag cccagcgcgt acaggaaggt
    15361 ggttgtgcga tccggtgcgc tggtggaggc cagcacttca cacactttca ggaagtcggc
    15421 ttttggcgta ccgcaaatgt tttctactac gtccggcgtg tagcgggaaa cgtgcgcttt
    15481 cagcaggttc catacacagc gcggatgagt cagcgtgtca tcgcgtttcg catagccgtt
    15541 ttcatcgaac tgatagttcc aggacgactt atcgtattga cgtttttcag cgtcgtagcc
    15601 gctgaacaga ccgtcttcga aagcaaaatc atcacgcacc agcaggctgg cgttggtgta
    15661 atgcttaacg tattcggcgt tgattttgtt gttttcgatc aggtagcgca aaacgccaga
    15721 caggaacgta atgtccgtac cggaacgaat aggtgcgtaa atatccgcca cagaagcggt
    15781 acgcgtaaaa cggggatcga caacgatcaa ggttgcatcg ttgttgtttt tcgcttccat
    15841 cgcccagcgg aaaccgacgg gatgcgcttc agcagcgtta ccgcccatca ccattacgac
    15901 gttagcgttt ttgatatcca cccagtggtt ggtcatcgca ccgcgaccaa atgttggagc
    15961 aagacttgct accgttggtc cgtgtcagac gcgcgcctgg ttgtctaccg ccagcatccc
    16021 gagggagcgg gcaaattttt gagtcagcat cccggtttcg ttgctggcac cggaagcaca
    16081 cagcataccg gtagaaagcc aacggtttac cgttacaccc tgctcgttct tttcaataaa
    16141 gttagcgtca cggtcggctt tcatcagctt cgcaatacgg gagaatgctt cttcccagct
    16201 aatgcgctgc catttgtcag aacctggcgc acgatattcc gggtagcgca gacggttttc
    16261 gctgttgacg taatccagca aaccggcccc tttcgggcac agcgcaccac ggcttaccgg
    16321 atgatccggg tcaccttcaa tgtgataaat cgcttctctg gcgtttttcg cgccatcacc
    16381 caggctatac atcaatagcc cgcaacctac ggaacagtat gtgcaggtgt tacggatctc
    16441 tttcgcgcgt aataatttgt agtttcgcgc ctgagccagt gcttgcttcg gggcaaagcc
    16501 caatgctgct actgttgttc ccgccatacc gcccgcgcag attttaaaaa attgtctgcg
    16561 actgacgtcc attgctttcc tcttttttca ggggtattac tgcgcgagga aactaacagt
    16621 aaaattctga atctttttgc gcgaaatcaa aaagaacacg tgtttgccac aaaataatcc
    16681 gcctgcgcaa attgacctac ctcaatagcg gtagaaaaac gcaccactgc ctgacaggcc
    16741 agttaaaaaa aatgctataa aattcagctt aatttttaac ggtaagagag acaaaacagc
    16801 gagcatgaca cgtcaaaaag caacgctcat agggctaata gcgatcgtcc tgtggagcac
    16861 gatggtagga ttgattcgcg gtgtcagtga gggactcggc ccggtcggcg gcgcagctgc
    16921 tatctattca ttaagcgggc tgctgttaat cttcacggtt ggatttccgc gtattcggca
    16981 gatcccgaaa ggctatttac tcgccgggag tctgttattc gtcagctatg aaatctgtct
    17041 ggcgctttcc ttagggtatg cggcgacccg tcatcaggcg attgaagtgg ggatggtgaa
    17101 ctatctatgg cccagcctga caattctctt tgccattctg tttaatggtc agaaaaccaa
    17161 ctggctgatt gtaccaggat tattattagc cttggtcggc gtctgctggg tgttaggcgg
    17221 tgacaatggg ttacattatg atcaaattat taataatatc accaccagcc cattgagtta
    17281 tttcctggcg ttcattgggg cgtttatctg ggcagcctac tgcacagtaa cgaataaata
    17341 cgcacgcgga tttaacggaa ttaccgtttt tgtcctgcta acgggagcaa gtctgtgggt
    17401 ttactatttt cttacgccac aaccagaaat ggtatttagc acgcccgtca tgattaaact
    17461 catctctgcg gcatttacct taggatttgc ttatgctgca tggaatgtcg gtatattaca
    17521 tggcaacgtc accataatgg cggtaggttc gtattttacg cccgtccttt cctcagcgct
    17581 tgcagccgtg ctgctcagcg ccccgctgtc gttctcgttc tggcaaggcg cgctaatggt
    17641 ctgcggcggt tccctgctct gctggctggc gacacgtcgt ggctaaaata attgtcgggt
    17701 ctacggcccg gcagttaatc agataaattc tgacaatatt aaaaaataaa tttcaaatac
    17761 cagaaagtca ttttagttac atttagtaac acatatctgt ctgtttaatc cacattctgc
    17821 ccccgcagtt acgctttctt atctattctt ctaatcagca cttcacctga ttcatcctta
    17881 attctaataa ctggctattg cgccttacct cctgttttgt ctttttttca ttaccaatgt
    17941 gtgcatgtga ggaacaatat ggcactgcaa aatgagaaaa atagtcgtta tcttttacgc
    18001 gactggaaac cagaaaatcc ggccttctgg gaaaataaag gaaagcatat tgcacgaaga
    18061 aacctctgga tatcagtcag ttgcctactt cttgccttct gtgtctggat gctatttagc
    18121 gcagttactg ttaatctcaa taaaatcggt tttaatttca ctaccgatca actcttttta
    18181 ttaaccgcat taccctccgt ttctggcgca ttattgcgtg tcccctactc ctttatggtg
    18241 cctatattcg gtggacgccg atggacggtt tttagtactg caatcctgat tattccttgc
    18301 gtctggctcg gaattgccgt gcagaatccg aatactcctt ttgggatatt tatcgttatc
    18361 gctttgctat gcggttttgc aggtgcgaac tttgcttcga gcatgggcaa tatcagtttc
    18421 ttctttccaa aagccaaaca agggagcgct cttgggatta atggcggatt aggaaactta
    18481 ggtgtaagtg tgatgcagct ggttgcaccg ctggtcattt ttgtccccgt atttgccttt
    18541 ctcggcgtca atggcgtacc gcaggccgac ggttcggtga tgtcgctggc gaatgccgca
    18601 tggatttggg tgccattact ggcgattgcc acgatcgccg cctggtcagg gatgaatgat
    18661 atcgccagtt cacgcgcctc aattgccgac cagctccctg tcttacaacg cctgcatctc
    18721 tggctgctga gcctgcttta tcttgccacc ttcggttcgt ttatcggttt ttctgcgggt
    18781 tttgccatgc tggcaaaaac ccagttcccg gatgtgaata ttctgcgcct ggcgttcttt
    18841 ggcccattta tcggtgccat cgcgcgatcg gttggtggtg ctatttccga taaattcggc
    18901 ggcgtgcggg tgacgttgat caacttcatt tttatggcga ttttcagcgc cctgctgttc
    18961 cttaccttac cgggcacagg ctccggtaat ttcatcgcat tttacgccgt atttatgggg
    19021 ctgtttctga ccgcgggtct gggaagtggt tctactttcc agatgatcgc tgtcatcttt
    19081 cgccagataa caatttatcg ggtgaagatg aaaggcggta gtgatgagca agctcaaaga
    19141 gaagccgtca ccgaaacggc agccgctctg ggctttatct cagccattgg cgcagtgggc
    19201 ggctttttta ttccgcaggc gtttggcatg tcgctcaata tgaccggctc tccggtgggc
    19261 gcgatgaaag tgtttttaat cttctacatc gtttgtgtgc tactgacctg gctggtttat
    19321 ggtcggcgga agttcagcca aaaataagag atcgatgcct gacacgtctt atcaggctac
    19381 aaccgtgtcg catccgacaa ggcatcagat cccaattcat tcctggagca ggagttatgt
    19441 catgagtaaa cttttggatc gctttcgcta cttcaaacaa aagggcgaaa cctttgccga
    19501 tggtcacgga caggtgatgc atagcaaccg cgactgggag gacagctatc gccagcgttg
    19561 gcagttcgac aaaatcgtgc gttccaccca cggtgttaac tgtacaggct cctgtagctg
    19621 gaaaatctac gttaaaaatg gtctggtgac ctgggaaatc caacagaccg actacccgcg
    19681 cactcgccct gacctgccaa atcatgaacc tcgcggctgc ccgcgtggcg caagttactc
    19741 ctggtatctc tacagcgcta accgcctgaa atacccgctc attcgtaaac gactgattga
    19801 actgtggcgc gaagccctca agcaacacag cgatccggta ctggcgtggg catcgattat
    19861 gaacgatccg caaaagtgcc tgagctacaa acaagtgcgt ggacgcggtg gctttatccg
    19921 ctccaactgg caggagctaa atcagctgat tgccgctgct aacgtctgga ccatcaaaac
    19981 ctatggcccg gatcgcgttg ccggattctc accgattccg gcgatgtcga tgatttctta
    20041 cgccgccgga acacgctatc tgtcgctgtt aggtggcacc tgtttaagct tctacgactg
    20101 gtattgcgac ctgccgcccg cctcgccgat gacctggggc gagcaaaccg acgtaccgga
    20161 atctgccgac tggtataact ccagttacat catcgcctgg gggtctaacg taccgcagac
    20221 acgtacgccg gacgcccatt tctttaccga agtacgctac aaaggcacta aaaccatcgc
    20281 cattacccct gactactctg aagtggccaa attgtgcgac cagtggctgg caccgaaaca
    20341 aggcactgat agtgccctgg caatggcaat gggccatgtc attttaaaag agtttcatct
    20401 cgataatccc agcgattatt ttatcaacta ctgccgccgc tacagcgaca tgccgatgct
    20461 ggtaatgctg gaacctcgcg acgatggcag ctacgttccc gggcgcatgg tccgcgcatc
    20521 tgacctggtg gatggactgg gcgaaagcaa taatccgcag tggaaaaccg tggcagttaa
    20581 taccgcaggt gaactggtgg tgccgaacgg ttcgattggt tttcgctggg gagaaaaagg
    20641 caaatggaac ctggaatcca ttgccgccgg tacggaaacc gaattgtcgt taaccctgct
    20701 cggtcaacac gacaccgtcg caggcgtggc cttcccctac tttggcggca ttgaaaatcc
    20761 gcactttcgc agcgtaaaac atgatccggt actggtgcgc cagcttccgg tgaaaaatct
    20821 gacgctcgcc gatggcactg tctgtccggt ggtcagcgtt tatgatttgg tactggcgaa
    20881 ttacggcctc gatcgcgggc tggaagatga aaacagcgcg aaagattacg ctgaaatcaa
    20941 accttatacc cctgcctggg gcgagcaaat taccggcgtt ccgcgccagt atattgaaac
    21001 catcgcccgt gaatttgccg ataccgccca taaaacgcat gggcgctcga tgattattct
    21061 cggcgcaggt gttaaccact ggtatcacat ggacatgaac taccgcggga tgatcaatat
    21121 gctgatcttc tgcggttgtg tcgggcaaag cggtggcggc tgggcacact atgtcggtca
    21181 ggaaaaactg cgcccgcaaa ccggctggct gcctctggcc tttgcgctcg actggaaccg
    21241 accaccgcgc cagatgaaca gcacctcgtt tttctacaat cactccagcc agtggcgcta
    21301 tgaaaaagtc accgcgcagg aattgctctc accgctcgcc gatgccagta aatacagcgg
    21361 tcacctgatt gatttcaacg ttcgcgccga acgtatgggc tggctacctt ctgcgccgca
    21421 gctggggcgt aacccgctct ccctgaaagc tgaagccgac aaggccggat tatctcccac
    21481 agaatttacc gcccaggcgc tgaaatcggg cgatttacgc atggcctgcg aacagccgga
    21541 caacggcagc aaccatccgc gtaacctgtt tgtctggcgt tcaaacctgc ttggctcttc
    21601 cggcaagggc cacgagtata tgcagaagta tctgctgggt acggaaagtg gcattcaggg
    21661 ggaagaactt ggaccgacgg agggaatcca accggaagaa gttgagtggc aaactgcggc
    21721 gattgaaggc aagctcgacc tgctggtgac gcttgacttc cgcatgtcca gcacctgcct
    21781 gttctccgat atcgttctgc ccaccgccac ctggtacgaa aaagacgata tgaatacctc
    21841 ggatatgcat ccgtttattc atccgctttc tgcggcggtc gatccggcgt gggaatcacg
    21901 cagcgactgg gaaatctaca aaggtatcgc taaagccttt tcgcaagtgt gcgtggggca
    21961 tctcggcaaa gaaaccgacg tggtattaca accactgctg catgactccc cggcagagct
    22021 ttcacagccg tgtgaagtgc tcgactggcg caaaggcgag tgcgatctga ttccaggcaa
    22081 aaccgcgccg aatattgtgg cggtggagcg cgactaccct gctacctatg aacgctttac
    22141 ctcgctcggg ccattgatgg acaaacttgg caacggcggt aaagggattt cgtggaatac
    22201 ccaggatgaa attgatttcc tcggtaaact taattacacc aagcgtgatg gcccggcaca
    22261 ggggcgtccg ctgattgata ccgcgattga cgcatcagaa gtgattctgg cactggcacc
    22321 ggaaaccaac ggccatgttg cagtcaaagc gtggcaagcg ctgggcgaga tcaccggacg
    22381 cgaacatacc catctggcgc tgcacaaaga ggacgagaag attcgctttc gcgatattca
    22441 ggcgcagcca cgtaaaatta tctccagccc cacctggtct ggtctggaaa gcgatcacgt
    22501 ctcctataac gcgggataca ccaacgttca tgagttaatt ccgtggcgca cgctgtcggg
    22561 acgccagcaa ctctatcagg atcatccgtg gatgcgtgct tttggtgaaa gtctggtggc
    22621 ttatcgcccg cctatcgaca cccgcagtgt cagtgaaatg cgccagatac cgccaaacgg
    22681 cttcccggag aaagccctta acttcctgac accgcaccag aaatggggca ttcactcaac
    22741 ctacagtgaa aacctgctaa tgctgacgct ctctcgcggt ggaccgattg tctggatcag
    22801 cgaaaccgat gcccgtgaac tgaccattgt cgataacgac tgggtggaag tgtttaacgc
    22861 caatggcgcg ctgacggccc gcgcggtggt cagccagcgt gtaccaccgg gcatgaccat
    22921 gatgtatcac gctcaggaac gcattatgaa tattccaggt tcggaagtaa ccggcatgcg
    22981 cggcggcatt cataactcgg tcacccgcgt ttgtccgaaa ccaacgcata tgattggcgg
    23041 ttacgcgcag ctggcctggg gctttaacta ctacggcact gtcggctcta accgcgacga
    23101 attcatcatg atccgcaaga tgaagaacgt taactggctg gatgatgaag gtcgcgatca
    23161 ggttcaggag gcgaaaaaat gaaaatacgt tcgcaagtcg gcatggtgct taacctcgat
    23221 aaatgtatcg gctgccatac ctgttcggtg acctgtaaaa acgtctggac cggacgcgaa
    23281 ggcatggagt acgcatggtt taacaacgtc gaaaccaaac cgggcattgg ttatccgaaa
    23341 aactgggaag atcaggatga gtggcaaggt ggctgggtgc gtgatgtgaa tggcaagata
    23401 cgcccgcgtc tgggcagcaa gatgggcgta ataaccaaaa ttttcgccaa cccggtagtg
    23461 ccgcagattg acgattacta cgaacctttc accttcgact acgaacattt gcatagcgcg
    23521 ccggaaggca aacatattcc caccgcccgt ccgcgttcac tgattgacgg caaacggatg
    23581 gacaaagtga tctggggccc aaactgggaa gaactgctgg gcggcgaatt tgaaaaacga
    23641 gcccgcgacc gcaacttcga ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc
    23701 ttcatgatgt atctgccgcg cttgtgcgaa cactgcctta accccagttg tgtagcgacc
    23761 tgccccagcg gcgctatcta caaacgcgaa gaagacggca ttgtgctgat cgatcaggat
    23821 aaatgccgtg gctggcgttt gtgcattagc ggttgcccat acaaaaaaat ctacttcaac
    23881 tggaaaagcg gcaagtcaga aaaatgtatc ttctgttacc cacgaattga gtccggtcaa
    23941 ccgaccgtgt gctcagaaac ctgcgtgggt cgcatccggt atttgggcgt gctactttac
    24001 gacgccgacc gcatcgagga agcggcgagc accgagcgcg aagttgacct ctatgaatgc
    24061 cagtgcgaag tgttcctcga tccgcacgat ccctcggtga tcgaagaagc cctgaaacaa
    24121 ggtattccac aaaacgtgat tgacgctgcc cagcgttcgc ctgtctacaa aatggcgatg
    24181 gactggaaac tggcgctacc gctgcaccct gaatatcgca ccctgccgat ggtctggtac
    24241 gttcctccgc tgtcaccgat tcagtcctac gctgatgcgg gaggtttgcc gaaaagcgaa
    24301 ggcgtgctgc ccgccatcga aagcctgcgt attccggtgc aatatctcgc caatatgttg
    24361 agtgccggtg ataccggtcc ggtactgcgg gcgctgaaac ggatgatggc gatgcgccac
    24421 tatatgcgtt cacaaaccgt ggaaggcgtt actgatactc gtgccatcga cgaagtaggt
    24481 ctgagcgtcg cccaggtcga agagatgtat cgttacctcg ccattgccaa ctatgaagat
    24541 cgtttcgtca tcccgacaag ccatcgggaa atggcgggcg atgccttcgc agaacgcaac
    24601 ggctgcggtt ttacctttgg cgacggttgc cacggttcgg acagcaaatt caacctgttc
    24661 aacagtagcc gtatcgatgc catcaacatc accgaagtgc gcgacaaagc ggagggcgaa
    24721 taatgcagat cctcaaagtg ctcggcctgt tgatggagta cccagacgag ttgttgtggg
    24781 agtgtaagga cgacgcgctg gcgcttatcc gccgcgacgc gccgatgctc accgattttg
    24841 cactcgacct gctcaacgcc ccgctgctgg ataaacaggc cgaatggtgt gaagtgttcg
    24901 accgcgggcg caccacgtcg ctgctgctgt ttgaacatgt tcatgccgag tcccgcgatc
    24961 gcggccaggc aatggtggac ctgctggcgg agtatgaaaa ggtcggtctg caactgaatt
    25021 gtagggaact gcccgattat ctaccgctgt atctggagta tttaagcgta attccggacg
    25081 atcaggcgaa agaaggatta ctcaacgttg cgccgatcct cgccctgctt ggcggacgct
    25141 taaaacaacg cgaggcaccg tggtacgcgt tatttgacgc cctgttgcaa ctggcaggaa
    25201 gccccctgac aagtgacagt gtcacaaaac agattcgcag tgaagagcgt gacgacaccc
    25261 gccaggcact ggatgcggtg tgggaagaag aacaggtgaa gtttattgaa gataacgcca
    25321 cggcgtgtga cagctcgccg cttaatcaat atcagcgacg ctttagccag gatgtcgcgc
    25381 cgcagtatgt cgacatcagt gcgggaggtg ggaaatgatt cagtatctga acgtcttttt
    25441 ttacgatatc tacccgtact tgtgtggcac ggtttttatc cttggaagct ggctgcgcta
    25501 cgactacggg cagtacacct ggcgcgccgc ctccagccag atgctggata aacgcgggat
    25561 ggtgctgtgg tcaaacttgt tccatatcgg cattttgggg attttcttcg ggcacctgtt
    25621 cggcatgtta acgccacact ggatgtacgc gtggtttctg ccggtggcgg taaaacagca
    25681 gatggcgatg atcctcggcg gaatatgcgg ggtattgacg ctgattggcg gtgcggggct
    25741 actggtgcgt cgtttaacaa atccgcgtat cagggcgacg tcttctggtg cggatatcct
    25801 gatcctcgcc attttgttaa ttcagtgcat tcttgggtta accaccattc ctttttcagc
    25861 acaacatccg gacggcagtg aaatgatgaa gctggtgggc tgggcgcaaa gcattgtcac
    25921 tttccagggc ggtgcatcta cgcatcttga tggtgtcgcc tttatattcc gcgttcatct
    25981 ggtgcttgga atgaccatct tcctgctctt cccgttcacc cgactggtgc atgtctggag
    26041 cgcgccgttt gagtacttta cccgtcggta tcagattgtg cgttcgcggc gttaattctc
    26101 acgcgggccg gatagccctt cgctctccgg ccacttgcag gctatgatag ttctccagca
    26161 acaggagaac attaatgaaa ccgcaggttt accacgtcga tgcttttacc tctcaaccgt
    26221 ttcgcggcaa ttctgccggt gttgttttcc ccgccgataa tctcagcgaa gcgcaaatgc
    26281 agcttatcgc ccgcgagtta ggtcattcgg aaactgcttt tctgctgcac agcgacgaca
    26341 gcgatgtgcg catccgctac tttacgccaa cggttgaagt tccgatttgt ggtcacgcga
    26401 cagtagctgc gcactatgta cgtgcaaagg tgttaggttt aggaaattgc acggtctggc
    26461 aaacatcgct ggcaggaaaa catcgtgtga ctatagaaaa gcaaaatgat gattatcgta
    26521 tttcgctgga acaaggtact ccaggctttg agccaccgct ggaaggtgaa acacgtgcag
    26581 cgattatcaa cgcgcttcat ctcactgagg acgatattct gccaggcttg ccaattcagg
    26641 tggcaacaac aggacactca aaagtgatga ttccactgaa gccggaagtg gatatcgacg
    26701 ccctttcgcc tgaccttgct gcgctgaccg caatcagcaa gcagattggc tgcaatggtt
    26761 tcttcccgtt ccagatccgc ccaggcaaaa gcgaaaccga cggtcgcatg ttctcgcctg
    26821 cgattggtat tgtggaagat ccggtgaccg ggaatgccaa tggtccgatg ggagcatggt
    26881 tagtacatca caacgtatta tcccacgatg gcaacgtgtt gcgtgttaaa ggccatcagg
    26941 ggcgcgcatt ggggcgtgat ggtgtaattg atgtgacggt gacaattcga gataaccaac
    27001 cggaaaaagt taccatttcc ggcacggcgg tgattctatt ccatgctgaa tgggccattg
    27061 atttttgatg ccgttgcctg atgcgcttcg cttatcaggt ctacaaccgg gctaatgtat
    27121 gccggataag gcgtttacgc cacatccggc aacctgacat aaattatttt cccgcctccg
    27181 ggtgcgtatc aaacgccgcc atcaccagcg ctaactcatc cacagtaaag ccatgtttcg
    27241 catcatctac gcccagacca aactgttcct gcatcacggc atataacgac gccacatctg
    27301 gtagatttcg ctgctcaact gcgtgcccat tttcataatg ggtaaaatga aaattggtca
    27361 gcgttagctt gccaccgtcc ggcaaatggc gacacatcag caaatgatgg cgaaaatgcg
    27421 actgcggcca gagcgccgac cagaaattgc ccatcacata atcgctttgt tgctgctcgc
    27481 agagatcaaa acggtacatc gactgccagt gctgatgatg attaaactgc aatacccaat
    27541 catcaccctc ttgcaacaac cgatactctc cgtgtggcgt ggtttgcatg atatccggca
    27601 ttaaacgaat cggcgcggtt agcgtctgcc caccgaaacc gacatcagca atccattttt
    27661 ccccttccag ttccaccaac agcaaacgat gggtgcgcgg cggtaatgct ggcggatttg
    27721 ataacactac gcgacctaac aagctgcgaa cgttaaaccc cagctcgcgt aacacccgct
    27781 caaacacgcc attctgctca aaacagtaac cgccacgacg ggctatcacc agtttatctt
    27841 ccagcgattg atcatcaagc tgtatttccc tcggcagcaa aacgtcgaga ttttcaaacg
    27901 gaatggtgca attgtgtttc aggtgcaatg cacgcagcgt atcaatattg gccgcagcag
    27961 ctcccaacca gttaatacgg gcaaaataat gattcagaat gggcgtcatc aggcgtttcc
    28021 cttagtggtt ttctcaactt ataacctgga atagacgccg cactggctat tttgtgcgat
    28081 cgcggacatg catcacgttc gcaaatcgcc aatcccgtta atttcgttac ttattgcgtt
    28141 acgagctacg cttttaaaac acccttcata gcgaggcgat atcgtgagcc aattcattcc
    28201 cgtcgaatta caccatgcca gccgtctgtt aaatcatggc ccaactgtca tgatcaccag
    28261 tttcgatgag caatcccagc ggcgtaacat tatggcagca gcctggtcga tgcccgtaga
    28321 gtttgaaccg ccacgcgtgg cgattgtggt agataaatcg acatggacca gggagttgat
    28381 tgaacgtaac ggtaagtttg gcatcgttat cccgggcgtt gcggcgacta actggacgtg
    28441 ggcggtggga agtgtgtcgg gtcgtgaaga agataaattt aattgctacg gcattccggt
    28501 agtgagaggc ccggtgcttg gtttgccgct ggtcgaggaa aaatgtctgg cgtggatgga
    28561 gtgtcgcctg ctacctgcaa cttccgcgca agaaaaatac gatacgctat ttggcgaagt
    28621 ggtatcagca gcggcagacg cacgggtatt tgtcgaaggc cgctggcagt ttgatgatga
    28681 taagctcaat acgttgcatc atttaggtgc tggcacgttt gttaccagtg gtaagcgagt
    28741 tacggcaggt taagcattac atgctgtaac cgggtttctt tatcaaagcc tccatttggg
    28801 gcgcgatttc ggcatcccag atagcttgcc aggattctgg ttgaatctgc tgtagagaaa
    28861 tgcttatcga gctatcttta ctgttcagat ggcgaataat aacgtcgtta atatctgcag
    28921 caagcgctgc tttttgttgt tcgtccagtt cacgcggaaa acatttaatg tcgatgtgcg
    28981 gcatgattgt gctccttttg aaagatgtca gtaaaattaa catacattgt gaatgtcgtg
    29041 tgtccggtga tgcgttttta cgacaataat gcctacttga aattgtagat atttgcacta
    29101 caccctttta aataaagcat caacaaatcg tcaaattgca gaacatattc ttctgccaac
    29161 ataccattaa gaaaacatca agttcccgac ttgccaattt attaacgccc attatagacg
    29221 ctgaaacaag ccacgggcaa aggagatgcc aaaattaccg gcaatgcaga aaaactcaac
    29281 gaacttttag gttaaatgga taattttgaa tttttgcacc gtctcctcgg tcagcaactg
    29341 tcccagtctg tcgtaactga agcagataat ctccggtgcg tccgtttccc gggcatccga
    29401 ccatctgcag cggatttcca gtagcaggtc tgccgcatca tagacatatt ccgtacggct
    29461 atctgctgtc tcctttgcca tgagctggcc cactgcatca cgcgccagtg ctcaaaatca
    29521 gcgccaaatg gctgagagaa gcgggatttg agatcggcac gggcgttacc gtgaagatct
    29581 cgaaaggttg tctgatcctg cttgcggata acaacgaagt acaggaactg cgcagggaac
    29641 tttatcaggt taaacaggca attaagggga tgcgtgacgg gatgtttagt gtgctgaacg
    29701 agagttaaat aataaaaagc cgggaagatt taatttctta cccggctttt attttaatat
    29761 ttttcttaat ttcttttatc catacattaa actctttta
//
LOCUS       DAZFJL010000050        29549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFJL010000050 DAZFJL010000000
VERSION     DAZFJL010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.050"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            229..456
                     /gene="feoA"
                     /locus_tag="ACHJ8C_002141"
     CDS             229..456
                     /gene="feoA"
                     /locus_tag="ACHJ8C_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO5551997.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            473..2794
                     /gene="feoB"
                     /locus_tag="ACHJ8C_002142"
     CDS             473..2794
                     /gene="feoB"
                     /locus_tag="ACHJ8C_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO5551998.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGSRQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDVELLATRKSVSSCCAASTTGDCH"
     gene            2805..3041
                     /gene="feoC"
                     /locus_tag="ACHJ8C_002143"
     CDS             2805..3041
                     /gene="feoC"
                     /locus_tag="ACHJ8C_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO5551999.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLKQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLHEWWALR"
     gene            3274..3867
                     /locus_tag="ACHJ8C_002144"
                     /pseudo
     CDS             3274..3867
                     /locus_tag="ACHJ8C_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            complement(3896..4666)
                     /gene="bioH"
                     /locus_tag="ACHJ8C_002145"
     CDS             complement(3896..4666)
                     /gene="bioH"
                     /locus_tag="ACHJ8C_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO5552000.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEVVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVLMPFLRLYGYFDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPDEFCHLLVALKLRV"
     gene            4704..5387
                     /gene="gntX"
                     /locus_tag="ACHJ8C_002146"
     CDS             4704..5387
                     /gene="gntX"
                     /locus_tag="ACHJ8C_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO5552001.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPLPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLNARLRKHNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            5446..6021
                     /gene="nfuA"
                     /locus_tag="ACHJ8C_002147"
     CDS             5446..6021
                     /gene="nfuA"
                     /locus_tag="ACHJ8C_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO5552002.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            6385..7701
                     /gene="gntT"
                     /locus_tag="ACHJ8C_002148"
     CDS             6385..7701
                     /gene="gntT"
                     /locus_tag="ACHJ8C_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO5552003.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(7812..9896)
                     /gene="malQ"
                     /locus_tag="ACHJ8C_002149"
     CDS             complement(7812..9896)
                     /gene="malQ"
                     /locus_tag="ACHJ8C_002149"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO5552004.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGKQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEALAWWKLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMADPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(9906..12299)
                     /gene="malP"
                     /locus_tag="ACHJ8C_002150"
     CDS             complement(9906..12299)
                     /gene="malP"
                     /locus_tag="ACHJ8C_002150"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO5552005.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRGNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQSNRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            12922..15627
                     /gene="malT"
                     /locus_tag="ACHJ8C_002151"
     CDS             12922..15627
                     /gene="malT"
                     /locus_tag="ACHJ8C_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO5552006.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICDTMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFESAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            15694..15972
                     /locus_tag="ACHJ8C_002152"
     CDS             15694..15972
                     /locus_tag="ACHJ8C_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO5552007.1"
                     /translation="MKPESKETPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACRE
                     AQDILLDQRLFILDDEQYDAFLAALDAPITAERQAKINALMNRKSPWE"
     gene            15963..16448
                     /locus_tag="ACHJ8C_002153"
     CDS             15963..16448
                     /locus_tag="ACHJ8C_002153"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5552008.1"
                     /translation="MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSSLSRV
                     YVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAPESLPVVVLGRLAIDQAWAGKGL
                     GVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
                     E"
     gene            complement(16498..17526)
                     /gene="rtcA"
                     /locus_tag="ACHJ8C_002154"
     CDS             complement(16498..17526)
                     /gene="rtcA"
                     /locus_tag="ACHJ8C_002154"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO5552009.1"
                     /translation="MKRMIVLDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGVHQQTTLLRHDFY
                     PAGGGMVATEVSPVASFNSLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGTGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VMITKLTD"
     gene            complement(17530..18756)
                     /gene="rtcB"
                     /locus_tag="ACHJ8C_002155"
     CDS             complement(17530..18756)
                     /gene="rtcB"
                     /locus_tag="ACHJ8C_002155"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO5552010.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSVTQKTVKQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            18975..19589
                     /gene="rtcR"
                     /locus_tag="ACHJ8C_002156"
                     /pseudo
     CDS             18975..19589
                     /gene="rtcR"
                     /locus_tag="ACHJ8C_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(19571..20329)
                     /locus_tag="ACHJ8C_002157"
     CDS             complement(19571..20329)
                     /locus_tag="ACHJ8C_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO5552011.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTMPPASVMQVLKDHHIQLELC"
     gene            complement(20346..21176)
                     /gene="glpG"
                     /locus_tag="ACHJ8C_002158"
                     /pseudo
     CDS             complement(20346..21176)
                     /gene="glpG"
                     /locus_tag="ACHJ8C_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
     gene            complement(21221..21547)
                     /gene="glpE"
                     /locus_tag="ACHJ8C_002159"
     CDS             complement(21221..21547)
                     /gene="glpE"
                     /locus_tag="ACHJ8C_002159"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO5552012.1"
                     /translation="MDQFECINVTDAHQKLQEKEAVLVDIRDPQSFAMGHATQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            21737..23242
                     /gene="glpD"
                     /locus_tag="ACHJ8C_002160"
     CDS             21737..23242
                     /gene="glpD"
                     /locus_tag="ACHJ8C_002160"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO5552013.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     HKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYTWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLNRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(23296..23901)
                     /locus_tag="ACHJ8C_002161"
     CDS             complement(23296..23901)
                     /locus_tag="ACHJ8C_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5552014.1"
                     /translation="MNKIRLYIYPVILGFVIVPLLVWPTVITLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELLVEYALFFSVMCGIGWQFSRRTPPELKNRLHCCLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCQWRSKNRSHRKKWGDWVTCLSLAILSLGPLFNYSQ"
     gene            complement(23904..24671)
                     /locus_tag="ACHJ8C_002162"
     CDS             complement(23904..24671)
                     /locus_tag="ACHJ8C_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5552015.1"
                     /translation="MYPTPAMQALIEQVYHIFRRYPVPQKFVVCCEYCLSQQEQKALR
                     NTSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVVQGQFDNIHEVFSLRRIN
                     LASKENWREDEREILQQFACQYMTDWVSGDEAVELQYMLEMFFRADIALSPLLDAIIS
                     VPGFWSAASLACLLNTYRDGYIRDNQDDIDKAITTQTNTWASNNQSILKERAQQAIEN
                     PLKQSEQGTQYQAWEDEWMIDECLCAMYDASSESSGQ"
     gene            complement(24674..25384)
                     /locus_tag="ACHJ8C_002163"
     CDS             complement(24674..25384)
                     /locus_tag="ACHJ8C_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5552016.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQV
                     LDEGEKFAGAAGLEGLSRYDVTLNIGLTSSNVWPGFSLDTATISRLCACGADFGFDLY
                     ISDVPDVQCDLNTTNDFTVQFTAMFNPDERVIIAKRLLKKCDAWIEDVYIFQVFKEAW
                     QFQNDNSLRGFRDKQAELKLYARHYSVENCTEESCWDCNYCIRPSFSLSRSAIIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(25558..26688)
                     /locus_tag="ACHJ8C_002164"
     CDS             complement(25558..26688)
                     /locus_tag="ACHJ8C_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5552017.1"
                     /translation="MRIHTYWYRRYFILLIIIFSNVLSSIANAEDMGRERAYCYPGSP
                     SNNTTPASFSYNFGTIVVSDVNKNALGTVLPSQIWKVGTYKAYCNSLDDYEIYFSAVS
                     GIDPSGASGDHQGSDVFIPLTHEISVSTHIKLYNQNGTMTDKIVPFENYNTNYPGDRS
                     KPSNWASGTEGYIKIRIDKKIISDVSLSNVLLVSLYVSQIPTEHGPIPVFNAYIGNLN
                     IQVPQGCTINEGTSFTVNMPDVWASELSRAGAGAKPAGVTPVATTIPINCTNKDTDAV
                     MTLVFDGNISATRDTNGKQSIIQAQDNPDVGIMIMDSQQNSVDLNALATSVGVPFRLV
                     ENQAASAQTADVTFLALPVSTTGKVPAAGRYNALAILRVEYQ"
     gene            complement(26707..27483)
                     /locus_tag="ACHJ8C_002165"
     CDS             complement(26707..27483)
                     /locus_tag="ACHJ8C_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5552018.1"
                     /translation="MRQYRLLSPMIKVVIVLFSLLFSTAQAGLVIGGTRFAYPENQSS
                     ISVELKNTSDRDMLVKVAVSPDDARHLTGTVESLPSFSGTVFIATPPLFVLKPDKNTK
                     IRINRVGGSLPADRESLFILNVAALPTLENSHSVKTDNQLQIAVRNRMKIFYRPSNLS
                     EDPNMSYQKLRWARKNEIVTVYNPGPRYVTLYNLHADGKVMDGGMIAPFSHRQQSWCK
                     AQGVCEIAWQTLDIYNNVLPILKVKLNPSQLDVTGFVFSQ"
     gene            complement(27449..28021)
                     /locus_tag="ACHJ8C_002166"
     CDS             complement(27449..28021)
                     /locus_tag="ACHJ8C_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5552019.1"
                     /translation="MPGKQMLCCILISIISEGDMKIFISLFLFIISTSSFADDITHAG
                     VVRIEGLITEKTCIISDESKNFTVNMPDVPSSSVRSAGDVTEKVYFPITLTRCGSDVG
                     NAYIKFTGNTVSEDASLYKLEEGSVEGLALTIFDKNKGSISNDVKSMGFSLTPSVDNI
                     LHFFAAYKALKNNVQPGDANASVSFIVTYD"
     gene            complement(27972..28535)
                     /locus_tag="ACHJ8C_002167"
     CDS             complement(27972..28535)
                     /locus_tag="ACHJ8C_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5552020.1"
                     /translation="MNLIFSKYSVFFIFFAFFAFPLSGQANDSVVVTFTGILLAKSCD
                     ITTGSKDQSVKMGTYDANEFLNVGDVSPSQMFTINVQGCPTAKSTVYSSGVSANVRFT
                     GDADAINSALLRISAGADSATGLGIEILDNNDVAIAINGESGFRDLVLNENGDANLTF
                     KLRYKSTQENVHAGQANALLYFDIDYQ"
     gene            complement(28535..>29549)
                     /locus_tag="ACHJ8C_002168"
     CDS             complement(28535..>29549)
                     /locus_tag="ACHJ8C_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5552021.1"
                     /translation="YLSASQQTYWNTDKKDSLYQVGYNTSIKGIYLNVVWNYSKSPGT
                     NADKIVSLNVSLPISNWLSSTNDGRSSSNAMTATYGYSQDNHGQVNQYTGVSGSLLEQ
                     HNLSYNIQHGFANQDNSSSGSVGVNYRGAYGSLNSAYSYYNEGNQQINYGISGALVVH
                     ENGLTLSQPLGETNVLIKAPGANNVDVQRGTGISTDWRGYAVVPYATEYRRNNISLDP
                     MSMNMHTELDITSTEVIPGKGALVRAEFAAHIGIRGLFTVRYRNKSVPFGATASAQIK
                     NSSQITGIVGDNGQLYLSGLPLEGVINIQWGDGVQQKCQANYKLPETELDNPVSYATL
                     ECR"
BASE COUNT         7392 a         7735 c         7006 g         7416 t
ORIGIN      
        1 attatatttt cgctgcgata taatcttgag ccatatcaac attgagtcag attattattc
       61 aaaccaacat tcacacacat tttaagtatt gctgatagaa accattctca ttatcattgt
      121 actgttgatt atttaatctc tccttcgttg gcaaatcatc tggtctcatg tcgctgtcaa
      181 acgccccatg aggtagttat ccagttaatg agaaacaagt aggcacctat gcaatacact
      241 ccagatactg cgtggaaaat tactggcttt tcccgtgaaa tcagcccggc atatcgccaa
      301 aaactgcttt ctcttggcat gttacctggc tcctctttta atgtggtgcg cgtcgctcca
      361 ctcggcgacc ccattcatat cgaaacccgt cgtgtgagcc tggtattacg caaaaaagat
      421 ctggccttat tagaagtgga agcggtttcc tgttaatacg gtgataacaa caatgaaaaa
      481 attaaccatt ggcttaattg gtaatccaaa ttctggcaag acaacgttat ttaaccagct
      541 cactggctca cgtcagcgtg taggtaactg ggctggcgtt accgtcgaac gtaaagaagg
      601 gcaattctcc accaccgatc atcaggtcac gctggtggac ctgcccggca cctattctct
      661 gaccaccatc tcatcgcaga cctcgctcga tgagcaaatc gcctgtcact acattttgag
      721 tggcgacgca gacctgctga ttaacgtggt ggatgcgtct aaccttgagc gtaacctgta
      781 cctgacgctg caactgttgg aactcggcat tccctgcatc gtggcgctga acatgctcga
      841 cattgccgag aagcaaaata ttcgcattga aattgatgcg ctgtcggcgc gtcttggctg
      901 cccggtgatc ccgctggttt caacccgtgg tcgcggtatt gaagcactca agctggcgat
      961 tgatcgctat aaagctaacg aaaatgtgga actggtgcat tacgctcagc cgctgctcaa
     1021 cgaagcagat tcactggcaa aagtgatgcc ttccgacatc ccgctgaaac aacgtcgctg
     1081 gctgggcctg caaatgctgg aaggcgatat ctacagccgc gcctacgccg gtgaagcgtc
     1141 gcagcatctg gatgccgccc tcgcccgtct gcgtaatgag atggacgatc cggcgctgca
     1201 cattgccgat gcgcgttacc agtgcattgc tgccatctgt gatgtggtaa gcaacaccct
     1261 gacggcagaa cccagccgtt tcaccactgc ggtagataaa atcgtgctca accgtttcct
     1321 cggtctgccg attttcctct ttgtgatgta cctgatgttc ttgctggcta tcaacatcgg
     1381 cggggcgtta cagccgctgt ttgacgtcgg ctccgtagca ctctttgtgc atggtattca
     1441 gtggattggc tacacgctcc acttcccgga ctggctgact atcttcctcg cccaaggcct
     1501 gggcggtggt attaacaccg tgctgccact ggtgccgcag atcggcatga tgtacctgtt
     1561 cctctccttc cttgaggact ccggctatat ggcgcgtgcg gcgtttgtga tggaccgtct
     1621 gatgcaggcg ctgggcttgc cggggaaatc ctttgtaccg ctgatcgttg gtttcggttg
     1681 taacgtaccg tcagtaatgg gcgcacgtac gcttgatgca ccgcgtgaac gtctgatgac
     1741 catcatgatg gcaccgttta tgtcctgcgg cgcgcgtctg gctatcttcg cagtattcgc
     1801 ggctgccttc ttcgggcaga acggtgcgct ggcggtcttc tcgctgtata tgctgggtat
     1861 cgtaatggcg gtgctgactg gcctgatgct caagtacacc atcatgcgcg gtgaagcgac
     1921 gccgtttgtc atggagctgc cggtctacca tgtaccacac gttaaaagcc tgattatcca
     1981 gacctggcag cgtctgaaag gcttcgttct gcgtgctggt aaagtgatta tcatcgtcag
     2041 cattttcctg agcgccttca acagcttctc gctgagcggg aaaatcgtcg ataacatcaa
     2101 cgactcggcg ctggcttccg tcagccgggt gatcaccccg gtcttcaagc cgattggcgt
     2161 acatgaagat aactggcagg caacggttgg cctgtttaca ggtgccatgg cgaaagaagt
     2221 ggtggtgggt acgctcaaca ccctctacac cgcagaaaat attcaggacg aagagttcaa
     2281 tccggcggaa tttaacctcg gtgaagagct gttcagtgcg gtagatgaaa cctggcagag
     2341 cctgaaagac accttcagcc ttagcgtact gatgaacccc attgaagcca gcaaaggcga
     2401 cggcgaaatg ggtaccgggg cgatgggcgt gatggatcag aaattcggta gcgcagctgc
     2461 cgcatacagc tacctgattt tcgtcctgct gtacgtaccg tgcatctcgg tgatgggggc
     2521 tatcgcgcgt gaatcaagcc gtggctggat gggcttctcc atcctgtggg ggctgaatat
     2581 tgcttactca ctggcaacat tgttctatca ggtcgccagc tacagccagc atccaactta
     2641 cagcctggtg tgcattctgg cagttatcct gtttaacatc gtggttatcg gtctgctgcg
     2701 ccgcgcgcgt agccgggtag atgtcgaact gctggcaacg cgcaaatcgg taagcagttg
     2761 ctgtgcggcc agcaccactg gcgattgcca ctaaggacac gacaatggct tcgctcattc
     2821 aagtgcgtga tttactggca ttacggggcc gtatggaagc ggcccagatt agccagacac
     2881 tgaacactcc gcagccaatg attaacgcca tgctgaaaca gctagaaagt atgggaaaag
     2941 cagtgcggat tcaggaagaa cctgacggct gtctttccgg cagttgtaaa agctgcccgg
     3001 aaggaaaagc ctgtctgcac gaatggtggg cgttgcgtta accttgtttc actgcctgtt
     3061 ttgaaagccc ggtatgcgtt tgcatccggg cttttttgcg tgcggctttc cataaaaatg
     3121 caacccttgc agcgcgacgt aagttcctct gaaagcgact cgcagggatg aaaactcgct
     3181 aatgcacagg tgtggagtgg cgcgtagagt cgcggcattc aaataacagg tgaaggaacg
     3241 ccatgagcaa aaagcagagt tccaccccac acgatgcgtt acgccattgc cgcaatgcaa
     3301 aatcaccttg atgccggata caaaacattg ccgatggtgg tgccattgtt gttttatcac
     3361 ggtattgaaa gcccctatcc ctattcgctg tgttggctgg attgtttcgc cgctcccaac
     3421 ctggcaaggc agctttatgc ctctgcattt ccgctgattg atatcaccct catgcctgat
     3481 gatgaaatca tgctgcaccg acgtatggcg ctactggagt taattcaaaa acatattcgt
     3541 caacgcgacc tgatggggct ggtagagcaa atggcctgct tattaagtag tggatacgct
     3601 aatggcagac aaatcaaagg gctgtttaat tacatactgc aaaccggcga cgcggtacgt
     3661 tttaacgatt ttatcgatgg tgtggccgaa cgttcaccaa aacacaagga gagtttaatg
     3721 actattacgg aaagattgcg gcaggaaggg gaacaatcca aagccttgca tatagcaaaa
     3781 ataatgcttg aatccggagt ccctcttgca gacatcatgc gctttaccgg agtgtcagaa
     3841 gaagagttgg ctgcggcgag ccagtaaagt tctgtcccgc catttcgaaa gccacctaca
     3901 ccctcagctt caacgccacc agcagatgac aaaactcgtc cggatgtgaa ataaacggcg
     3961 catgtgccgc tttggcgaag atatatgatt cgctgtgagg ccaaagttta tccagcatcg
     4021 gcaccacttt gcgcggcacc agaccgtcga aatagccata caatcgcaaa aacggcatga
     4081 gcacgttttg cagcggctga cggagatcga ccgttttcag gatttccagc ccgccattaa
     4141 gcacgtcaac ctccggcatc ggtaacgcca gaacggtttt cttcaacgcc cgcgcatcct
     4201 ggcgcgccgt ttcagtaccc atggtttgta acgccaggaa ccgctccact gtacgctgaa
     4261 aatcatcact gagttgctgc tgaaatcccg ccagcacgtc cggttttatc cccggccact
     4321 cgtcgcgagc actaaaacaa ggtgacgacg ccacggtgac cagcgcctga acccgctcgg
     4381 gatgggttaa cgcaatctgg cttgccacca gcccgcccag actccagcct aaccaaatgg
     4441 ctttatctgg tgcctgtcgc agcacgactt cggccatatc agcaagtgat agcgcaccaa
     4501 atccccggct acgcccgaag ccgggcaggt caacaaggtg cagcgtaaaa tgcgagctaa
     4561 gttcctcgtc aatgcaacgc cacacttcgg cattcagtcc ccatccgtgc agcagcacaa
     4621 gatgaacatt cccctgacct ttggtctgcc accagatgtt attcatccgc tattgttctc
     4681 tttttactta caaggatgaa catatgctaa cagtaccggg attatgctgg ctatgccgaa
     4741 tgccactggc gttaggtcat tgggggattt gttcggtctg ctctcgcgcc gcccgtacag
     4801 ataaaacgtt atgcccacaa tgtggattac ccgccaccca ctctcatctt ccctgcggtc
     4861 gctgcctgca aaaaccgctg ccctggcaaa gactggtcac ggtcgccgac tatgcgccgc
     4921 cattaagtcc gctcattcac cagcttaagt tttcccggcg cagtgaaatc gccagcgccc
     4981 tgtcacgtct gttactattg gaagtcttac acgctcgtcg caccaccggc ttgcaactgc
     5041 ctgatcgcat catcagcgtt ccgttatggc agcgccgtca ctggcgtcgg ggatttaatc
     5101 agagcgattt gctgtgtcag ccgttatcac gctggctaca ctgccaatgg gatagcgaag
     5161 ccgtcacacg aacacgggcc accgcgaccc agcattttct taacgcccgg ctacgcaaac
     5221 acaacctgaa aaatgccttt cgccttgaat tgcctgtgca aggtcgccat atggtgattg
     5281 tggatgatgt cgttaccacc ggaagtaccg tcgcagagat tgcgcagttg cttttacgca
     5341 atggtgcggc gactgtccag gtctggtgcc tttgtcgaac cttgtagagc ctcgatgatg
     5401 ggcgtattat aaccaactaa aatagtcaac tattaggcca ttactatgat ccgtatttcc
     5461 gatgctgcac aagcgcactt tgccaaactg ctggcaaatc aggaagaagg gacacaaatc
     5521 cgcgtatttg tgattaaccc tggcacgcct aacgctgaat gtggcgtttc ttattgtccg
     5581 ccggacgctg tggaagccac cgacactgcc ctgaaatttg acctgctgac tgcgtatgtt
     5641 gatgagttaa gcgctcctta cctggaagat gcagagatcg attttgttac cgaccagttg
     5701 ggttcgcagc tgacgctgaa agcgcctaac gccaaaatgc gtaaagtggc tgacgatgcg
     5761 ccgctgatgg agcgcgtgga gtatatgctg caatcacaga tcaacccaca gcttgctggt
     5821 cacggtggtc gcgtttcgct gatggaaatc accgaagacg gttacgccat tttgcaattt
     5881 ggcggtggct gtaacggttg ttccatggtc gacgtcaccc tgaaagaggg gatcgagaag
     5941 cagttgctga acgaattccc ggagctgaaa ggtgtacgcg atctcaccga acaccaacgc
     6001 ggcgagcact cttactacta agttctctcc ccttttgtcg attgcccgat gtgacgctaa
     6061 agcgtcttat cgggcctgta cgttgcacga gattctgacg ttagcgcaga attacctcag
     6121 caaatccaat ctcttcgttt ccccacgata atatgaccaa cctctcataa tttaaattta
     6181 ccccgctctg gtgattctca agcacgtaat gttacccgta tcattctcat gggcaccaaa
     6241 cacactcata acatctgact acactaactc gttttagggg tcatcagccg tggtgccgta
     6301 acatctctgt tcccagttgg gacaaccagg attttccgtc agaaacctga cgttacccat
     6361 aacaaattaa aggccaggta aatcatgcca ttagtcattg ttgctatcgg tgtaatcttg
     6421 ttattactcc tgatgatccg cttcaaaatg aacggcttca tcgctctcgt cctcgtggcg
     6481 cttgctgttg gattaatgca agggatgccg ctggataaag ttattggctc catcaaagcc
     6541 ggtgtcggcg gaacgctcgg tagccttgcc ctgatcatgg gttttggtgc gatgctgggc
     6601 aaaatgctgg cagactgcgg tggtgcacaa cgtatcgcta ccacgctgat tgccaaattt
     6661 ggtaaaaaac acatccagtg ggcggtggta ctgaccggtt ttaccgttgg ttttgccctg
     6721 ttctatgaag tgggtttcgt gctgatgctg ccgctggtgt ttaccatcgc ggcttccgcg
     6781 aatattccac tgctgtatgt tggtgtacca atggcggctg cactgtctgt gacccacggc
     6841 ttcctgccac cgcatccggg tccgactgca attgccacca ttttcaatgc cgatatgggt
     6901 aaaactctgc tgtacggtac tattctggca atcccgaccg tgattctcgc cggtccggtt
     6961 tacgctcgcg tgttgaaagg tatcgataag ccgatcccgg aaggtctgta cagcgcgaaa
     7021 accttcagcg aagaagagat gccgagcttt ggcgtcagcg tctggacctc tctggtgccg
     7081 gtggttctga tggcgatgcg tgcgattgcc gaaatgatcc tgccgaaagg tcacgctttc
     7141 ctgccggtag cggagttcct cggtgacccg gtaatggcta cgctgattgc cgtgctgatc
     7201 gcgatgttca cctttggtct gaaccgtggt cgttcaatgg atcagattaa cgacacgctg
     7261 gtttcttcca tcaaaatcat tgcgatgatg ctgttgatta tcggtggtgg cggtgcgttc
     7321 aagcaggtgc tggtagacag cggcgtggac aaatacattg cttccatgat gcacgaaacc
     7381 aacatttctc cgctgctgat ggcctggtcg attgctgccg tactgcgtat cgcgctgggt
     7441 tccgcaaccg tcgcggcaat cactgcgggt ggtatcgcgg caccgctgat tgcaacgacg
     7501 ggtgttagcc cggagctgat ggttattgcg gttggttccg gtagtgtgat tttctctcac
     7561 gtgaacgatc cgggcttctg gctgttcaaa gagtacttta acctgactat cggcgagacc
     7621 atcaaatcct ggtcgatgct ggaaaccatt atctcggtgt gcggtctggt aggctgtctg
     7681 ctgctgaata tggtgatttg ataatactga tggccggatg cggcgtgaac gccttatccg
     7741 gcctacgatt cggcattaat tagtaggccg gataagacgc gtcaagcgtc gcatccggca
     7801 tcaaccgcac tctacttctt cttcgctgcc gctctgcgcc gtctgtccaa atccttcagc
     7861 aacttgttca caccatcatc ggcaaacatc gactcaagcg ttgcggaaag tttacgtcgc
     7921 cagtttttat actggtaact ggtgccggga atatttaccg gatctgccat atccagccag
     7981 tcttccggct gtagccctaa cagggcgctg ttgctatcgg cgatgtagcg ctgtaagcca
     8041 cgattcagcg tcggcgtcat cgacatcaac gatgccttat gcccggcacg ttttggcaga
     8101 caaccatatt tatgcagtgc atccagtagt ccttgcttcg ccagttcgcg atcctgatac
     8161 agaccgcgca gtaccacttc atccggatac aaccccagcg ttttgcccag cgttagatcc
     8221 ccgctctccc agtagccgcg tagcgtaggc aggtcgtgtg tcgccgcaac cgccatcgac
     8281 tgctctgggt acgctttcgg cgcgcggaat gtcttctcgt ggtcgttttc gaaatagagc
     8341 actttgtagg agtacacacc gctgctgcgc agcttaccga caatctctac cggtacggta
     8401 ccgagatctt caccaatcac catacagcga tgacgtttac tttcgagtgc cagaatcgag
     8461 agcagatcat ccaccggata gtgaacatac gcgccctgat ctgccgtctc gccatacggt
     8521 atccaccaca aacgcagcat cgacatcacg tgatcaattc gtaatgcgcc gcagttttgc
     8581 atattggcac gcaacagctc gataaacggt tcataggcac gcgcggtgat gatatgcggg
     8641 tccattggcg gcaatcccca gttctgcccc aacgggccga ggatatccgg cggcgcgcca
     8701 accgacgctt tcaggcaata cagttcacgg tcgcaccagg tttctgcccc accttctgct
     8761 acaccaacgg ccagatcacg atacaagcca atcggcattt catagccctg gcttatctcc
     8821 cagcaggcgg caaactggct gtaagccagc cactgcaacc agagataaaa atcgacgtca
     8881 tcacgatgtt cttcgcagaa ctggcgcact tctggtgaat ccacgttctg atacatctct
     8941 ggccatgctg gccagcccca gcgcatttcg tcctctttca cttgctgggc atgtagcgca
     9001 tcaaaggctg cctgccagaa caggctgtcg ccctgctctg caacaaactg gcgaaacgcg
     9061 gtcatctgct catcatcacg ttgcgcgaaa cctttccacg ccattcgtaa tgctgtcatt
     9121 tttagggcgg taaccgtgga gtaatcaacc cagtcggcat cccgcgcctg ttgcagcgtc
     9181 tgttgcgtgg tcggcaactt ccaccaggcc agagcctctt cgctaagatg gaaatcttca
     9241 acggcgttaa cgtcgatata aatcacattc agccaacggc gagaagacgg gctatacggg
     9301 ctggcacttt ccggatttgc cggatagagc gcatgaatcg ggttcagacc gatgaacgac
     9361 ccgccacgtt ttgccacatc caccagcatc gctttgagat cgccaaaatc cccaataccc
     9421 cagttttttt ccgatcgcag cgtataaagc tgaacgcagg caccccacag cttttgtttg
     9481 ttcagcaacg cctgcggttc gtaacagcgt ttcggggcga caatcacccg gcaatgcgcg
     9541 cgctggtcgt cctgggtaag cgtcagcgtg tgataacctt ccggcagctt cgtcggtaga
     9601 ttgaacgctt tgccccccgt cacatggcct ttgtactgct ttccttcttc ggtggtcagc
     9661 agccagctat attcgccgct gccctccacc accatcggca tttttttgcc gctggtataa
     9721 accatgacat tcgggactgg cgttaccgcc actttcgtgg cggtacgttg atgcatcgcg
     9781 tcaagcaaac gccgtttggt ttcggcgcta atcgactgcg gtttaccgtg ggcattgatg
     9841 taattggggc tgatccccgc cgccagcgcg gcattatcca gacgtttgct ttccatcgag
     9901 cttccttagc gttttgcctg ccagatacga gcctgataat cgcgaataga gcgatccgag
     9961 ctaaacatac cgcagcgggc ggtattgagg atcgccgcgc gagtccaggc ctcctggtcg
    10021 cggtacagca catccacctg cttttgtgcc tctacataag cggcgaaatc tgccatcacc
    10081 agatacggat cgccgccctg tttgccgata ctgtgcagca tctggtcgaa ggcatgctta
    10141 tcgccgtcgc tgtatttacc gctttccagc tctttcaata ctgcgtccag caccttatct
    10201 ttcttccgcc atttcaccgg gtcgtagcct ttggccagaa ttgccttcac ttgttccacg
    10261 gtatgaccaa aaataaagat attttcttca ccgactttct cagcgatttc aacgttcgca
    10321 ccatccagcg taccgacagt aagcgcacca ttgagcgcca gtttcatatt gccggtaccg
    10381 gaagcttctt tacctgcagt cgaaatttgt tcggagatat ccgccgccgg gatcagtttt
    10441 tccgccgccg aaacacaata atccggcaag aacaccacct tcaacttgtc gccaaccagc
    10501 ggatcgttgt tgatcacgtc agccactttg ttgatcgcaa agataatatt tttcgccagg
    10561 tagtagcccg gtgctgcttt cgcgccgaag aggaagacgc gcggtacgcg gttagactgc
    10621 gggttttcac gaatttcttt gtacaacgcc agaatatgca gcagattcag atgctggcgt
    10681 ttgtactcat gcagacgttt gatctgaata tcgaaaatcg cctgtggatt gatctcaata
    10741 ccggtacgaa ctttcacaaa ctctgccaga cggactttat tcgcctgctt gatctcgcga
    10801 tattgctgac ggaatttcgc atcatcagcg aatttttcca gattgatcag ttgatcgaga
    10861 tcgttagccc actctttttt cagtgattta tccaacagag ccgccagcgc cgggttgcac
    10921 tgtttgatcc agcgacgtgg ggtaataccg ttggtgacat tatggaattt gttcggccat
    10981 agctggtgat attccgggaa cagatctttc accaccagat ctgagtgcag cgccgcaaca
    11041 ccgttcaccg cgaaaccgcc aaccacacac aggttcgcca tatgcacttg tttgtcgtgc
    11101 accaccgcca gtttggccca cactttttca tcgcccggcc aggttttctc caccagcgtt
    11161 ttaaagcgag tattaatttc gttaataatc tgcatgtggc gcggcagtaa gcctttcacc
    11221 agtttcacat cccagcgttc cagcgcttct ggcatcaggg tatggttggt gtaagcgaaa
    11281 gttttgctgg tgatggccca ggcgtcatcc cagctcatct ggtgctcatc gatcagcacg
    11341 cgcagcagtt ctggaatcgc gatagttggg tgggtatcgt tcagctgaat aacttcgtaa
    11401 tccgccagtt cgtgcaattt acgccccgcc agatgatggc gacgcaaaat atccgctacc
    11461 gaacaggcac actggaagta ttgctgcatc aggcgcagct ttttaccggc agtatggttg
    11521 tcgtttggat agagaacttt ggtcagtttt tccgcgttga tgccctgctg ttcagcacgc
    11581 aggaaatcac catcgttaaa tttagtcaga tcaaacggat gcgcatgcgt cgcctgccac
    11641 agacgcagtg gctgcgccac gccattacga tagccgacaa cggggagatc ccacgcttga
    11701 ccagtaatgg taaattccgg ctcccagcgt ccgtctttcg tcactttacc gccaatccct
    11761 acctgcacat ccagtgcttc attgtggcgg aaccacggat agttaccacg atgccagtca
    11821 tccggcgctt caacctgttt gccatcgaca aatgactggc ggaacaagcc atattgataa
    11881 ttaaggccgt agccagtagc tgactgcccg acagttgcca ttgagtcgag gaagcacgcc
    11941 gccagacgtc ccagaccacc gttacccagc gccgggtcga tctcttcttc cagcaagtca
    12001 gtcaggttga tgtcataagc cttcaacgaa tcctgtacat cctgatacca gccgagattc
    12061 aacagattgt tgcccgtcag gcgaccaatc aaaaactcca ttgagatgta gttaacatgc
    12121 cgctgattcg ccaccggttt ggcgaatggc tgagcacgca gcatttcggc cagtgcttca
    12181 ctcactgcca gccaccactg gcgaggagtc atttcagccg cagaatttaa gccataacgc
    12241 tgccactgac gtgaaagcgc ttcctgaaat tgcttatcgt taaaaatagg ttgtgacata
    12301 ggagttccac ttttcttaga ttttcaacac aacgttatcg ctagtttgcc aggctcgatg
    12361 ttgaccttcc tcatcctgcg ggggattagg cagggaggag ttgcggggat gagcaaggaa
    12421 atgtgatctc gaccacttaa agctagtgca aaccacagga ttagcagcaa atcaatgcta
    12481 tacagcgcag aaaatctgta tctaagtgca aaaaatggcc gttgcgtatt ttcaaaaagc
    12541 ggaaggtaac tctataaatt aagtaaagga gtgaaacagt ttcacaagta aaatatcaaa
    12601 tgtgctccat ctcattctta atagatttat taagatcatc tttttagatg gcactttcat
    12661 cagaaatgaa gaagaaaccc ttgcttaaat gaaactgatg aacataagga aaataccgta
    12721 caacccagta ttcacgctgg atcagcgtcg ttttaggtga gttgttaata aacatttgga
    12781 attgtgacac agtgcaaatt cagacacata aaaaaacgtc atcgcttgca ttagaaaggt
    12841 ttctggccga ccttataacc attaattacg aagcgcaaaa aaaataatat ttcctcattt
    12901 tccacagtga agtgattaac tatgctgatt ccgtcaaaat taagtcgtcc ggttcgactc
    12961 gaccataccg tggttcgtga gcgcctgctg gctaaacttt ccggcgcgaa caacttccgg
    13021 ctggcgctga tcacaagtcc tgcgggctac ggaaagacca cgctcatttc ccagtgggcg
    13081 gcaggcaaaa acgatatcgg ctggtactcg ctggatgaag gtgataacca gcaagagcgt
    13141 ttcgccagct atctcattgc cgccgtgcaa caggcaacca acggtcactg cgcgatatgt
    13201 gacacgatgg cgcaaaaacg gcaatatgcc agcctgacgt cactcttcgc ccagcttttc
    13261 attgagctgg cggaatggca tagcccactt tatctggtca tcgatgacta tcatctgatc
    13321 actaatcctg tgatccacga gtcaatgcgc ttctttattc gccatcaacc agaaaatctc
    13381 acccttgtgg tgttgtcacg caaccttccg caactgggca ttgccaatct gcgtgttcgt
    13441 gaccagctgc tggaaattgg cagtcagcaa ctggcattta cccatcagga agcgaagcag
    13501 ttttttgatt gccgtctgtc atcgccaatt gaagccgcag aaagcagtcg gatttgcgat
    13561 gacgtttccg gttgggcgac ggcactgcag ctaatcgccc tctccgcccg gcagaatact
    13621 cactcagccc ataagtcggc acgccgcctg gcgggaatca atgccagcca tctttcggat
    13681 tatctggtcg atgaggtttt ggataacgtc gatctcgcaa cgcgccattt tctgttgaaa
    13741 agcgccattt tgcgctcaat gaacgatgca ctcatcaccc gtgtgaccgg cgaagaaaac
    13801 gggcaaatgc gcctcgaaga gattgagcgt cagggactgt ttttacagcg gatggatgat
    13861 accggcgagt ggttttgcta tcacccactg tttggtaact tcctgcgcca gcgctgccag
    13921 tgggaactgg cggcggagct gccggaaatc caccgtgccg ccgcagaaag ctggatggcc
    13981 cagggatttc ccagcgaagc tattcatcat gcgctggcgg caggcgatgc gctgatgctg
    14041 cgcgatattc tgcttaatca cgcctggagt ttgttcaacc atagcgaact gtcgctgctg
    14101 gaagagtcgc ttaaggccct gccgtgggac agtttgctgg aaaatccgca gttggtgtta
    14161 ttgcaggcgt ggctgatgca aagccagcat cgctacggcg aagttaacac cctgctggcc
    14221 cgcgctgaac atgaaatcaa ggacatcaga gaaggcacca tgcacgcaga atttaacgct
    14281 ctgcgcgccc aggtggcgat taacgatggt aacccggatg aagcggaacg gctggcaaaa
    14341 ctggcactgg aagagctgcc gccaggctgg ttctatagcc gcattgtggc aacctcggtg
    14401 ctgggtgaag tgttgcactg caaaggcgaa ttgacccgct cactggcgtt aatgcagcaa
    14461 accgaacaga tggcacgcca gcacgatgtc tggcactacg ctttgtggag tttaatccag
    14521 caaagtgaaa ttctgtttgc ccaagggttc ctgcaaaccg cgtgggaaac gcaggaaaaa
    14581 gcgttccagc tgatcaacga gcagcatctg gaacagttgc caatgcatga gtttctggta
    14641 cgcatccgtg cgcagctgtt atgggcctgg gcacggctgg atgaagccga agcgtcggcg
    14701 cgcagcggga ttgaagtctt gtcgtcttat cagccacagc aacagcttca gtgcctggca
    14761 atgttgattc aatgctcgct ggcccgtggt gatttagata acgcccgtag ccagctgaac
    14821 cgtctggaaa acctgctggg gaatggcaaa tatcacagcg actggatctc taacgccaac
    14881 aaagtccggg tgatttactg gcaaatgacc ggcgataaag ccgccgctgc caactggttg
    14941 cgtcatacgg ctaagccgga gtttgcgaac aaccacttcc tgcaaggtca atggcgcaac
    15001 attgcccgtg cacaaatctt gctgggcgag tttgaatctg cggaaattgt cctcgaagaa
    15061 ctcaatgaaa atgcccgcag tctgcggttg atgagcgatc tcaaccgtaa cctgttgctg
    15121 cttaatcaac tgtactggca ggccggacgt aaaagtgacg ctcagcgtgt gttgctggac
    15181 gcattaaaac tggcgaatcg taccggattt atcagtcatt ttgtcatcga aggcgaagcg
    15241 atggcgcaac aactgcgcca gcttattcaa ctcaatacgc tgccggagct ggaacagcat
    15301 cgcgcacagc gtattctgcg agaaatcaat caacatcatc ggcataaatt cgcccatttc
    15361 gatgagaatt tcgttgaacg tctgctaaat catccggaag tgccagagct gatccgcacc
    15421 agcccgttga cccaacgtga atggcaggtc ctggggctga tttactccgg ttacagcaac
    15481 gagcaaattg caggtgagct ggaagtggcg gcaaccacca tcaaaacgca tatccgtaat
    15541 ctgtaccaaa aactcggtgt cgcccaccgt caggccgcgg tacaacacgc gcagaaactg
    15601 ctgaagatga tgggatatgg ggtgtgagtt tgtatagcgt ttttatgtac actctttata
    15661 ctgtatataa aatcgcacta caggctcatg agtatgaaac cggaaagcaa agaaacacca
    15721 atcaatatca gggcgaaagc ctcacagcgt gacctgattg atatggcagc gaatctggtc
    15781 gcaaaatcgc gcactgattt tatgctggat gccgcatgcc gtgaagcgca agacatctta
    15841 ttagatcaac gactattcat ccttgatgac gagcaatacg atgcgtttct ggctgccctg
    15901 gacgccccca tcactgctga gcgtcaggcc aaaattaacg cgttgatgaa tcgtaaatcg
    15961 ccatgggaat aacggctcct actcccctaa cgtctgaaca taatctggct gacttttgct
    16021 gttccgatca cgggatgaat gaatggctaa aaaagaaagc gttaaaaaat cacagctcca
    16081 gtctgtctcg tgtttacgtt atctgtattg ccaatacccg ccaggtaatc ggctattact
    16141 gcctttctac aggcagtatt caacgtaatt tggcccctgg agccatgcga cgtaatgctc
    16201 ccgaatcgtt gcctgttgtc gtattaggca gactggcaat tgatcaagcc tgggcaggta
    16261 aagggctggg cgtcgcattg ttgaaagatg cagtctatcg cacaatgtcc atagctcaac
    16321 aggtaggggt tcgggcgctc attgttcatg ccttagacga ttcagtacgc aatttctatc
    16381 tgaagtatgc ctttgtacct tcaccattcc agtcacttac cttactgtat cccattactc
    16441 tggagtaatt tacctctacc ggatagtcac actgatgagt ctgatccggc aggggtttta
    16501 atcagtgagt ttggttatca tcacccgcgt tacgccatct gtttctatca aaccaaaccg
    16561 caccggcaag aaacgctcca ccacggcgat attggtcagc agatggcatg agggatgggc
    16621 gaccgtaaat tcccccgtac ccgccagcgc catcggtagc accagttggt cggcgagata
    16681 ttcccccacc gccgccgggc ttgccaggta gcgtttcacc tctttcacca actgtgccgc
    16741 gaccacctcg gcactgacgc gcttttcacc gacgacaaaa aagcgttcgg tgatattttc
    16801 actttcgact tcaagcgaga cggtattacc cggcccctgg tcgcgcggca ggttatgaat
    16861 attctgttca tgcaaggaaa aactacccgc cagtgtagcg atttcacgct cagcaacatg
    16921 gcgcggcaca ccagccagta gcacctctcc acgcatctgc acaatgttcc cgcgctcgcc
    16981 aagttgcaag ctattaaacg atgccaccgg tgagacttcc gttgctacca tgccgcctcc
    17041 ggcaggataa aaatcgtgac gtaacagcgt ggtttgctga tgaactccta ttttcgccag
    17101 cagcggctcc agcacccgac ggataaaatc cgcaggcggg gccgacgggt tatcggtgcc
    17161 gccgctcact tcaacacgcg aaggtccatc ggcaaaccac agcgcgggca gcaccgtttg
    17221 cagcaccagc gtacaacttc cggcgctacc gatagcaaag cggtaatcgc cgccgcgcac
    17281 ggtgccaggt cggaagacca gacgctgcga ccccagctca gcgccttcca ccgttgccct
    17341 acaaatttcc gcagccgctt ttaccgcggt cagatgttgg cgcaacagcc ccggtttcgc
    17401 ccgcccggca cgaatgccgg tgatggtaaa tggctggccg gttatcatcg acaggctcag
    17461 cgccgagcgc aggatctgcc cgccgccttc gccctgtgcg ccatccagca caatcatcct
    17521 tttcatcatt tatcctttta cgcacaccac ctgacgcagg gtatagataa cttccaccag
    17581 atcgctttgt gccgccatca ccgcatcaat atctttatac gccatcggga tttcgtcgat
    17641 cacctcggca tctttacggc actccacatg cgcggtggca cggatttgat cttccacact
    17701 gaacagtttt ttcgctttag tacggctcat tacccgcccg gcaccgtggc tgcacgaaca
    17761 gaacgactct tcatttccca gcccacggac gataaagctt ttcgccccca tcgaaccggg
    17821 aataattcca tattgacccg cacgcgcaga caccgcgcct ttacgcgtca catagatctc
    17881 ttcgccaaag tgctgttctt tttgcacata gttgtggtga cagttgatct cttccatcgc
    17941 cagcgtttgt ggctgtttta ccgttttctg cgtaacgctc tgcaacgccg ttaccacgtt
    18001 ttccatcatc gcatcgcggt taaggctggc aaaaagctgc gcccaggcca cggctttcag
    18061 gtaatcatca aagtattccg taccttccat aaagtacgcc aggtcacgcg atggcaacgt
    18121 ttcaagcgtt tcctgcatct ctttttgcgc cagatcgata aaataagttc cgatggcgtt
    18181 accaattccg cgtgaaccgg agtgcagcat aatccacacc tgatccgact catcaaggca
    18241 aatttcaata aagtggttac ccgtacccag cgttcccagg tgtttatagt tattagtgtt
    18301 aaggaaacgc ggatattttt gcgttaacca ctgataaccg gcctccagct cagcccattt
    18361 cgcatcgacg ttaacaggcg gattttccca tgcgccttta tcacgtttac aacgcccggt
    18421 agtacgtccg tgcggtacgg ccgtttcaat cgcctgacgc agctctgcca ggttttcagg
    18481 caggtccgcc gccgttaacg cggtacgcag tgcgttcatt ccacagccaa tatccacgcc
    18541 caccgccgcc ggaataatcg cccctttggt cgggatcacg ctaccaatgg tggaaccttt
    18601 acccaggtgt acatcaggca ttaccgcaat atgtttgaaa ataaatggca tcttcgcggt
    18661 attaataagt tgctgacgcg catcggcttc taccggcacg cctttggtcc acatttttac
    18721 cggagcattt tcagtggtca gtaattcgta attcattttg tttttctctt ttcgttgttt
    18781 gctgtcctga taattgcaac cgtcgtgcca gaaaatcaaa taagcatctt aattttttaa
    18841 ttcattgttt ttaaaaatat tatattttta cgtccgtaaa cggagatttc ccgcaaagcc
    18901 gatttaccga taatgaaatc tcgtctttta taaggatatc taagatgcgt aaaacagtgg
    18961 cttttggctt tgtcggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
    19021 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cttgccagga ttacgccaac
    19081 gccaggaaga tattgaaccg aacctggatt atgaagtgga gcgccacgcc tcactcactg
    19141 gcgacagcgt gcgttttaac accgaagcgc ggcgtgcctg gctggccttt gcgacctctc
    19201 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
    19261 cctttgccac tagcggacgc atcacactgg atacggtgga agatgagatc aaccgtctgc
    19321 gctataactg gcaggagagt cgcccctcca cgcttacggc gttgctgggc gcagaggcag
    19381 aaaacatcga tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgccagg
    19441 caaagtcgct ttctgctgcc ggacgtgaac tgtttgacat ttcgcgccag ggtaaagcca
    19501 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acatgggaag
    19561 caatgcagga tcagcacagc tccagttgaa tatgatggtc cttcagcacc tgcatcacgc
    19621 tcgcaggcgg catcgtgtcg gtgtagacag catcgaccat gctgatgcta cccatattga
    19681 ccattgcgtt acggccaaat tttgagtggt cgacaaccag cataacgtgg cgcgagttct
    19741 caatgatggc gcgtttggtg cgaacttcgt ggtaatcgaa ctccagtagc gagccgtcgc
    19801 tatcgatgcc gcttatcccc agaatgccga aatcgaggcg gaactgggag ataaaatcga
    19861 gcgttgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcaagaatga
    19921 tacggaaatc ctcttttacc atcagcgtgt tagcgacgtt gagattgttg gtcacaatgc
    19981 gtaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
    20041 taaacagcgt cgagccattg ggaatttgct ccgccacttt gcgggcgatg cgctcttttt
    20101 cttcggtctg ggtggccttg cgatcgtgcc acggcgtatt aaccgaactg gaaggcagcg
    20161 ccgcaccgcc atgatggcgc aggatcaggt tttgctctgc cagctcgttg agatcgcggc
    20221 gaatagtctg cgggctaacg gagaaatgct ctaccagctc ctcggtgctg acataaccct
    20281 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt gataaatccc
    20341 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
    20401 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
    20461 gcgacaatcc agatcaacgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
    20521 ggatcgcgtt cgccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
    20581 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
    20641 agagtaatga caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
    20701 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
    20761 aagtaacgcc agaactcaaa tttcagcgtc ggatcgaatg gctaggccag ccataacatc
    20821 acttcctgat cgccgagaat ttgcatggcg ataaacacca ccacgcaggc gatcatcatc
    20881 acccaggtta ccggacctgc gcgttcacgc aaggcagcaa agaaaggata acggcgataa
    20941 tgcaggccac tgtcggtatg accggactgc caactggcgg cgagataacg tggatctgcc
    21001 gggttttcga gaaaacgcgc cagctccgcc cgcacgcgtt cggcctggga ctcatccgcc
    21061 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
    21121 taatcaacaa acgcctgcgc cactcggggg ttagcaaaag aggtaatcat caacatcgtt
    21181 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
    21241 ctgcgggaaa ctggcgctgc caggcttcaa agccgccatc aatgctgtag accacatcgt
    21301 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
    21361 ccatcaccgg tgtgtcaaag tcgttatcac gcataaacgc gcccagtgtg tcgttagtta
    21421 aatggaaagc ctgggtcgca tgccccattg cgaaactctg tggatcgcga atatcgacca
    21481 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggt aacgttaata cattcgaact
    21541 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
    21601 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
    21661 acccaaagcg cgattctttg ctaacatgtt cgataacgaa catttatgaa ctttaacgaa
    21721 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
    21781 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
    21841 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
    21901 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctact
    21961 gaaaatggcc ccgcatatcg ccttcccgat gcgttttcgc ctgccgcatc gtccgcatct
    22021 acgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gcaagcgcac
    22081 cagcctgccg ggatcaaccg gtttgcgttt tggcgcaaac tcagtgttaa aaccggaaat
    22141 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
    22201 cgcccagatg gtggtacata aaggcggtga agtgcttact cgtactcgtg ccacatctgc
    22261 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
    22321 tacatggcag gcgcgcggcc tggttaatgc taccggcccg tgggtgaaac aatttttcga
    22381 cgacgggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
    22441 ggtgccgcgt gtgcataccc agaagcaagc ctacattctg caaaacgaag ataaacgtat
    22501 tgtcttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
    22561 caaaggcgat ccaaaagcag tgaagattga agagagtgaa atcaattacc tgttgaaagt
    22621 gtataacacg cactttaaaa agcagttaaa ccgggacgat atcgtctgga cctactccgg
    22681 cgtgcgtccg ctatgtgatg atgagtccga ctcgccgcag gccattaccc gtgattacac
    22741 ccttgatatc catgatgaaa acggcaaagc cccgctgctg tcggtattcg gcggtaagct
    22801 gaccacctac cgcaaactgg cggagcacgc gctggaaaaa ctgacgccat attatcaggg
    22861 cattggtccg gcgtggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
    22921 tgacgattac gccgcccgcc tgcgccgccg ttacccgttc ctgaccgaat cgctggcacg
    22981 tcactacgcg cgcacttacg gcagcaacag cgaactgttg ctcggcaatg caggggcgat
    23041 aagcgacctc ggggaagatt tcggtcatga gttctacgaa gcggagctga aataccttgt
    23101 ggatcacgaa tgggtccgcc gcgccgacga tgccctatgg cgtcgcacaa aacaaggcat
    23161 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggagt atacgcagca
    23221 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgtg aagtgcaacc gggctatttg
    23281 aattccttag actattcatt gtgaataatt gaataacggc cccagagata aaattgccaa
    23341 acttaagcac gttacccaat cgccccactt cttcctatgg cttctatttt tgctacgcca
    23401 ctggcaataa tatcccaacg caaaaccaag ctgtgcacta atcgggccga caaaatcagt
    23461 ccacggctgc ggccaaaaag gggaaaaatt caggggaagc cagacaaacg tcagaaagaa
    23521 atttcggata ttttccagca acgctgattt ctctggagaa atataaagaa tgaagttcga
    23581 gagaattaac cagaaataga ccggagaaaa gaccagacag caatgtaacc tgttttttaa
    23641 ttcaggaggc gttctgcggg agaattgcca accgatgcca cacattacac tgaaaaaaag
    23701 tgcatattca accaataact caagttgtaa ttcctgaaga gaaatacgca caaccaggag
    23761 cggaaaggag aatattattt cagccagaaa agttaacgtg aatataagta cggctaaggt
    23821 aataaccgtc ggccatacca ataatggtac gataacaaaa cccaaaatta cagggtaaat
    23881 ataaagtctg attttattca tctttattgt cctgatgatt cggatgatgc gtcatacatg
    23941 gcacataaac actcatcaat catccactcg tcttcccagg cctgatactg tgtgccctgt
    24001 tcggactgct taagcggatt ctcaatcgcc tgctgcgcgc gttctttcag gatggattga
    24061 ttattactag cccaagtatt agtttgagtg gtaatggctt tatcgatatc gtcctggtta
    24121 tctctgatat aaccgtcacg atacgtattc aataaacagg caagagacgc tgccgaccaa
    24181 aatccgggaa ctgaaataat ggcatcaagc aatggcgaaa gagcgatatc ggcacggaaa
    24241 aacatttcca gcatgtactg taattcgaca gcctcatccc cggataccca atcagtcata
    24301 tactggcaag caaattgctg caatatttcc cgttcatcct cacgccagtt ttctttactg
    24361 gcaaggttta tacgccgtag agaaaatact tcgtgaatat tgtcaaattg cccctgcaca
    24421 acaaactcca gtaaacgagg cagaaaataa cgaacctcat cgctgttttg cggatcaggt
    24481 cctggactac tattccaggc attgattaag gaatacggga tcgctcgtaa cgatgtattc
    24541 cgtaatgcct tttgttcctg ctgactcagg caatattcac aacaaaccac aaatttctgc
    24601 ggcaccggat aacgacgaaa aatatgataa acctgctcaa ttaatgcctg catggcagga
    24661 gtggggtaca taattacccc ctagcccgct gatcgcgtgt aaagggttga tagacaaaac
    24721 gagcattcgc cgcattcagg cgaataatgg cactacgaga gagggaaaag ctgggccgaa
    24781 tgcaataatt acagtcccaa cagctttcct ctgtgcaatt ttccactgag taatgacgcg
    24841 cgtagagttt cagttcagcc tgcttatcgc gaaatccgcg taaactgtta tcgttttgaa
    24901 attgccaggc ttctttaaac acctgaaaga tataaacatc ctcaatccag gcgtcacatt
    24961 ttttaagtag gcgctttgct atgatcaccc gctcatccgg gttgaacatc gccgtaaatt
    25021 gtacggtgaa gtcatttgtc gtgttcagat cacattgaac atctggtaca tcactgatat
    25081 agaggtcaaa tccaaagtca gcaccacagg cacaaagacg gctaatggtg gcggtatcga
    25141 gggaaaatcc cggccataca ttgctggacg ttaaaccaat atttaatgtg acatcatatc
    25201 gagataatcc ctcaagaccg gcagcaccgg cgaacttttc cccttcatcc aggacctgat
    25261 taatgagttg ctggacaatg ccttcatccc agtatgccga tttgcagttg gcaatcgtaa
    25321 tatccacgcc tgtgaaagga tcagtaccag cgaggcttgc aaataatgta ttaactaatt
    25381 ccatttttat tactcgcctt aatgcaacgt tttatacatc ggcacataac ctacattttc
    25441 caccaggttt ttcccctgta gaattaaaaa tatttttttg ttttacattc tgttcatccc
    25501 tattattgca ttttattttg cggtgatggc taaaccgatt ctgtactagc tcaaaaatta
    25561 ctgatattcc actcgcaata tagccagtgc attataacga cctgccgcag gcacttttcc
    25621 agttgtacta acaggtaaag ccaggaaagt aacatcagca gtttgcgcag aagcagcctg
    25681 gttttccacc aatctgaacg gaacgcctac tgatgttgcc agagcattta aatctacgga
    25741 gttttgctgg ctatccataa tcataatacc aacatcagga ttatcctgtg cctgaataat
    25801 gctttgtttc ccattggtat cacgtgtggc ggaaatgtta ccgtcgaata ccaacgtcat
    25861 taccgcatct gtatctttat tcgtacaatt aatcggaata gttgttgcta caggagtaac
    25921 accagcgggc ttcgctccgg caccagcccg gctcaattca ctggcccaca catccggcat
    25981 attaacagta aaactcgtac cctcattaat agtgcaacct tgcggaacct gaatatttaa
    26041 gtttcctatg taggcattaa agacagggat aggaccatgt tcggtaggga tctggctgac
    26101 atataatgac accaataata cgttacttaa tgaaacatca gatataattt ttttatcaat
    26161 cctgatttta atatatcctt cagtacctga tgcccaatta gatggtttac ttctgtcccc
    26221 cggataattg gtattataat tttcgaatgg cacaatttta tctgtcattg tgccattctg
    26281 attataaagt tttatatgag tagagacaga tatttcatgg gtgagtggaa taaatacatc
    26341 actcccttga tgatcaccac tggcaccaga cggatctatt ccagagacag cactgaagta
    26401 aatctcataa tcatcaagag aattacaata agccttatag gttccaacct tccagatttg
    26461 tgatggcaat acagtgccaa gcgcattttt gttgacatca gaaaccacta tagtaccaaa
    26521 attataagaa aaggatgcag gcgtagtatt attactcggt gaacccggat aacaatatgc
    26581 acgttctcgc cccatatctt cagcattagc aatagaagaa agaacatttg aaaatataat
    26641 aatcaataaa atgaaatatc ttctatacca ataagtatgt atacgcatat catgtcctca
    26701 gatatattat tgactaaata caaagccagt aacatccaac tgtgaaggat ttaattttac
    26761 tttcaaaatt ggcaatacat tattataaat gtccaatgtc tgccatgcta tttcacaaac
    26821 cccttgcgct ttacaccatg attgttgacg atggctaaat ggtgcgatca taccaccatc
    26881 cataacttta ccatcagcat gaaggttata gagtgtcaca tacctggggc ctggattata
    26941 aacggtaaca atttcatttt ttcgtgccca gcgtaatttt tgatatgaca tattggggtc
    27001 ttcagacaaa tttgatggcc gatagaatat tttcatacga ttgcggactg ctatttgcaa
    27061 ttgattatca gttttgactg aatgactatt ttccaacgtt ggtaatgctg caacattcag
    27121 tataaataaa ctttcccggt cggcaggtaa actgccacca acacgattaa ttcttatttt
    27181 tgtatttttg tcaggcttca aaacaaacag tggcggagtt gcgataaata ccgttccaga
    27241 aaatgagggc aaagattcaa ccgttcccgt caaatgacga gcatcatcag gggagactgc
    27301 aaccttgact aacatatctc tgtctgaagt atttttgagt tcaacagata ttgaggactg
    27361 attttccgga tacgcaaaac gcgttccacc gatcactaaa cctgcttgtg ctgtagagaa
    27421 taaaagggaa aataatacta tgactacctt aatcataggt gacaataaac gatactgacg
    27481 cattggcatc ccctggttgg acattatttt ttaatgcttt gtaagccgca aaaaaatgca
    27541 atatattatc aactgatggt gtaagtgaaa aacccatgct tttaacatca ttactaatac
    27601 tgcctttatt cttatcaaaa atcgtgagtg caagcccctc taccgagcct tcttccagct
    27661 tatataaact ggcatcttct gaaactgtat taccggtaaa ctttatataa gcgttgccaa
    27721 catcactacc acagcgggtt aacgttatgg gaaaataaac cttttcagta acatctcctg
    27781 cactccttac cgaactactg ggtacgtctg gcatattaac tgtaaaattt tttgactcat
    27841 cagaaataat gcaggttttt tcggtaatta acccttcaat acgaaccact ccggcatgag
    27901 tgatatcatc agcaaaagaa cttgttgata ttataaacaa aaataaacta atgaatatct
    27961 tcatatctcc ctcactgata atcgatatca aaatacaaca gagcatttgc ttgcccggca
    28021 tgtacatttt cctgagttga tttatagcga agtttaaacg tcaaattagc atcaccgttt
    28081 tcatttaaca ctaaatcacg aaaacctgat tcaccattaa tggcaatcgc cacatcattg
    28141 ttatccaata tttctattcc cagtccagtc gcactatctg caccggcgga gatacgcagt
    28201 aatgcgctat taatagcatc tgcatcacca gtaaaccgca cattggcgct cacaccagaa
    28261 ctataaacag tactttttgc agtcgggcat ccttgtacat taatcgtaaa catttgactc
    28321 ggagaaacat cacctacatt taaaaattca ttagcatcat aagtgcccat ttttaccgac
    28381 tgatctttac ttccggtagt aatgtcacag ctttttgcca acaaaatccc agtaaatgta
    28441 acaacaactg aatcattggc ctgccctgag agcggaaaag caaaaaaagc gaaaaaaata
    28501 aaaaatacag aatatttaga gaatattaaa ttcattagcg gcactccaga gttgcatagc
    28561 taacaggatt atccagttct gtttcaggga gcttgtaatt agcctgacat ttttgctgaa
    28621 caccgtctcc ccactggata ttaataacac cttctaaagg caatcctgag agataaagtt
    28681 gtccattatc gccgacaatc ccggtaattt gactactgtt tttaatctga gcgctggctg
    28741 tagcaccgaa tgggactgat ttattacgat aacgaactgt gaacaaacca cgaataccga
    28801 tatgagcagc aaactctgca cgaactaacg caccttttcc cggaataact tcagtggaag
    28861 tgatatccag ttcagtatgc atattcattg acataggatc taatgaaata ttattacgtc
    28921 tatattctgt tgcataagga acaactgcat atccacgcca gtcagtggat attcctgtcc
    28981 cccgctgaac atccacatta ttcgctccgg gcgcttttat caaaacatta gtttcaccta
    29041 atggttgact caacgtaaga ccattttcat gtacaacaag agcaccactg atgccatagt
    29101 ttatttgttg attaccttca ttatagtaac tgtaggcgga attcaaggaa ccatatgccc
    29161 cacgataatt aacaccaaca gaaccactac tgctattatc ctgattagca aaaccatgtt
    29221 gtatgttata actgagatta tgctgctcca acagagaacc agataccccc gtatattggt
    29281 ttacctgtcc gtggttatcc tgactataac catacgttgc agtcatggca ttcgatgatg
    29341 agcgcccatc attcgtggaa gataaccaat tacttatagg taatgagaca tttagcgaga
    29401 caattttatc cgcatttgtc cctggtgatt tactgtaatt ccacacaaca tttagataga
    29461 tacccttaat actggtgtta taaccaactt ggtataaaga atctttttta tcagtattcc
    29521 agtatgtttg ctgactagcc gataaatac
//
LOCUS       DAZFJL010000051        28870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFJL010000051 DAZFJL010000000
VERSION     DAZFJL010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.051"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            115..321
                     /gene="ybfA"
                     /locus_tag="ACHJ8C_002169"
     CDS             115..321
                     /gene="ybfA"
                     /locus_tag="ACHJ8C_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO5552022.1"
                     /translation="MELYKEYPAWLIFLRRTCAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            422..931
                     /gene="ybgA"
                     /locus_tag="ACHJ8C_002170"
     CDS             422..931
                     /gene="ybgA"
                     /locus_tag="ACHJ8C_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO5552023.1"
                     /translation="MNQQLFDDSTLIRIFALHELHNLKDRGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHRWQSLDDYYNQYRQRVIVLLSHPANRRDHTNVLMHAQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHAGVL"
     gene            928..2346
                     /gene="phrB"
                     /locus_tag="ACHJ8C_002171"
     CDS             928..2346
                     /gene="phrB"
                     /locus_tag="ACHJ8C_002171"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO5552024.1"
                     /translation="MTTHLVWFRQDLRQHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNVSPRQAELINTQLNALQNALAEKGIPLLFREVDDFAASVETVKQVCAENRITHLFY
                     NYQYEVNERARDVQAERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKFRSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQVLEGGPGSVWLNEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKAVHEPWKWAEKTGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(2388..3869)
                     /gene="dtpD"
                     /locus_tag="ACHJ8C_002172"
     CDS             complement(2388..3869)
                     /gene="dtpD"
                     /locus_tag="ACHJ8C_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO5552025.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYSD
                     NHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGF
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            4140..4883
                     /gene="ybgI"
                     /locus_tag="ACHJ8C_002173"
     CDS             4140..4883
                     /gene="ybgI"
                     /locus_tag="ACHJ8C_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO5552026.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            4906..5562
                     /gene="pxpB"
                     /locus_tag="ACHJ8C_002174"
     CDS             4906..5562
                     /gene="pxpB"
                     /locus_tag="ACHJ8C_002174"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO5552027.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAAGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLHVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPERDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5556..6488
                     /gene="pxpC"
                     /locus_tag="ACHJ8C_002175"
     CDS             5556..6488
                     /gene="pxpC"
                     /locus_tag="ACHJ8C_002175"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO5552028.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMRAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLRDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            6478..7212
                     /gene="pxpA"
                     /locus_tag="ACHJ8C_002176"
     CDS             6478..7212
                     /gene="pxpA"
                     /locus_tag="ACHJ8C_002176"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO5552029.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVFAQTLYQIGALAAITRAQGGVMCH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGERYGLVTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQYGRVKSITGEWAMVTAQTVCLH
                     GDGEHALAFARRLRATFAEKGIVVAA"
     gene            7248..8039
                     /gene="nei"
                     /locus_tag="ACHJ8C_002177"
     CDS             7248..8039
                     /gene="nei"
                     /locus_tag="ACHJ8C_002177"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO5552030.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKSYQSRLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDTPRLSYATRGQVDE
                     NKYHGALFRFKVFHRDGEPCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(8036..9082)
                     /gene="abrB"
                     /locus_tag="ACHJ8C_002178"
     CDS             complement(8036..9082)
                     /gene="abrB"
                     /locus_tag="ACHJ8C_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO5552031.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLALHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(9234..9629)
                     /locus_tag="ACHJ8C_002179"
                     /pseudo
     CDS             complement(9234..9629)
                     /locus_tag="ACHJ8C_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            complement(9756..11039)
                     /gene="gltA"
                     /locus_tag="ACHJ8C_002180"
     CDS             complement(9756..11039)
                     /gene="gltA"
                     /locus_tag="ACHJ8C_002180"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO5552032.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(11245..11352)
                     /locus_tag="ACHJ8C_002181"
     CDS             complement(11245..11352)
                     /locus_tag="ACHJ8C_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5552033.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            11733..12137
                     /gene="sdhC"
                     /locus_tag="ACHJ8C_002182"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             11733..12137
                     /gene="sdhC"
                     /locus_tag="ACHJ8C_002182"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO5552034.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPVTAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVFSLLAGVLVW"
     gene            12131..12478
                     /gene="sdhD"
                     /locus_tag="ACHJ8C_002183"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             12131..12478
                     /gene="sdhD"
                     /locus_tag="ACHJ8C_002183"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO5552035.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            12478..14244
                     /gene="sdhA"
                     /locus_tag="ACHJ8C_002184"
     CDS             12478..14244
                     /gene="sdhA"
                     /locus_tag="ACHJ8C_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO5552036.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            14260..14976
                     /gene="sdhB"
                     /locus_tag="ACHJ8C_002185"
     CDS             14260..14976
                     /gene="sdhB"
                     /locus_tag="ACHJ8C_002185"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO5552037.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            15432..18233
                     /gene="sucA"
                     /locus_tag="ACHJ8C_002186"
     CDS             15432..18233
                     /gene="sucA"
                     /locus_tag="ACHJ8C_002186"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO5552038.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQEKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            18248..19465
                     /gene="odhB"
                     /locus_tag="ACHJ8C_002187"
     CDS             18248..19465
                     /gene="odhB"
                     /locus_tag="ACHJ8C_002187"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO5552039.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPTLAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            19559..20725
                     /gene="sucC"
                     /locus_tag="ACHJ8C_002188"
     CDS             19559..20725
                     /gene="sucC"
                     /locus_tag="ACHJ8C_002188"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO5552040.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            20725..21594
                     /gene="sucD"
                     /locus_tag="ACHJ8C_002189"
     CDS             20725..21594
                     /gene="sucD"
                     /locus_tag="ACHJ8C_002189"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO5552041.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(21698..22420)
                     /gene="mngR"
                     /locus_tag="ACHJ8C_002190"
     CDS             complement(21698..22420)
                     /gene="mngR"
                     /locus_tag="ACHJ8C_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFO5552042.1"
                     /translation="MGRKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLTDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITPQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQFMENS
                     KYQFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            22590..24506
                     /gene="mngA"
                     /locus_tag="ACHJ8C_002191"
     CDS             22590..24506
                     /gene="mngA"
                     /locus_tag="ACHJ8C_002191"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFO5552043.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTAEQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLERSGETRTVQQDT
                     QPAKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVLAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            24524..27157
                     /gene="mngB"
                     /locus_tag="ACHJ8C_002192"
     CDS             24524..27157
                     /gene="mngB"
                     /locus_tag="ACHJ8C_002192"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFO5552044.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILYRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADEDGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEITHEAWQSRAVIR
                     YDMAVPRNLHERSARQSTGRVGVELAVTLSDNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNNSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGLAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGSFSC
                     RLSLFSYTGTPSTAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVVLRLFNPAESTTCEATVAFSREVISCTQTMMDESISTAERD
                     NLKELGAFLPGQSRTFSYRLG"
     gene            complement(27302..28699)
                     /locus_tag="ACHJ8C_002193"
     CDS             complement(27302..28699)
                     /locus_tag="ACHJ8C_002193"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HFO5552045.1"
                     /translation="MHHLSRLDKAFLRKGIQLSRANNIVSLSRPSGARARVLLPDELP
                     LEEKAVTQLLDFAEVKLPDHPGYVRQTCATPDFHPGNGVPVGAVVASTPDLVIPAAIG
                     TDISCGMRLLTTGLTLEQAEANKEIITQRLTQVLLQNERDVPLSARAFTALFDGGPAD
                     CMSALPSAGLWTRTNIPRMVQEIDRCIGLNEHNSHSRYAPPALLATDRALLRDPCLGT
                     PGSGNHFIELQVVDSVVDRHVAWQQGLKTGDIVVMIHSGSRDVGFYVGRRWMDRARAE
                     WPTGHKHPQSGLYGLAGGLAEEYLQAIGMAARYAWLNRVTIAEMVRVCLANLFHQDNS
                     HLVVDVPHNIILREHEMNIHRKGATPARVGNLALIPGSMGDYSWLAVGCGNPEWLWSC
                     SHGAGRSQRRQAMRSRATEESTLPWQCVTLREERRFEEAPAAYKDIGPVIEAQQEAGL
                     IQPAVRFRPWLTFKG"
BASE COUNT         7059 a         7196 c         7892 g         6723 t
ORIGIN      
        1 taagtggtcg gatgagtagt aaaattacac aaacagcggt actattttca tcagataaac
       61 gaattcattt ttccggtgct attcaccggg tagatacaaa agggggagaa aagtatggaa
      121 ctctacaaag aatatcctgc atggcttatc tttttacgcc gtacttgtgc ggttgcagcg
      181 ggcgttctgg cgctgccttt catgcttttc tggaaagacc gcgcccgctt ttatagctac
      241 ctgcaccgcg tctggtcgaa aaccagcgat aaaccggtgt ggatggatca ggccgaaaag
      301 gcaaccggtg atttttactg attgccaaac atgacgttta aagaaaaccg ccggtggaaa
      361 cgctggcggt tttctttttt cggcgtctac acttactcct gtaaaccgtt caggagcagt
      421 aatgaatcaa caactttttg atgacagcac ccttatccgc atttttgctt tgcatgagtt
      481 acacaatctc aaagatcgag gactaacgcg cggggcgctt ctcgattatc acagccgcta
      541 taaactcgtc tttctggcac attctcagcc ggagtaccgc aaacttggcc cgtttgtggc
      601 tgatattcac cggtggcaaa gtctggatga ctattacaac cagtaccgcc aacgcgtaat
      661 tgttttgctt tctcaccctg ccaaccggcg tgatcatact aacgttttga tgcacgctca
      721 gggatatttt cgcccacata ttgattccac agaacgccag cagctggcgg cgcttatcga
      781 cagttatcgc cgtggcgagc aaccgcttct tgcgccgctg atgcgtatca aacactatat
      841 ggcgctttat cctgacgcct ggctttcagg gcagcgttat ttcgaacttt ggccgcgtgt
      901 gattaacttg cgccatgcag gagttttatg actacccatc tggtctggtt tcgccaggat
      961 ttacgtcagc acgataatct cgcgctggct gccgcctgcc gcaattcgtc tgcgcgcgtg
     1021 ctggcgttgt atatcgctac accacgccag tgggcggcgc ataatgtgtc gccgcgtcag
     1081 gctgaactta tcaatacaca acttaatgcg ctgcaaaacg cgctggcgga aaaaggcatt
     1141 cctttattgt tccgcgaagt ggatgacttt gctgccagtg tcgaaacagt taaacaggtg
     1201 tgcgcggaaa atcgcattac ccatctgttt tataactatc agtatgaggt gaatgagcgg
     1261 gcgcgggatg tgcaggctga gaggacgctg cgtaacgtgg tgtgtgaagg atttgatgac
     1321 agcgtgatcc tgccgccggg ggcggtgatg actggcaatc atgagatgta caaagtcttt
     1381 acgcctttta aaaacgcatg gttgaaacgg ctgcgggaag ggatgccgga gtgcgtcgct
     1441 gcaccaaaat ttcgtagtag cggatcgata gagcccgcgc catccattac gctgaattat
     1501 cctcgtcagt ctttcgatac tgcgcatttc ccggtggaag aaaaagcggc gattgcgcaa
     1561 ttacgccagt tttgccagaa cggtgccgga gagtatgagc aacaacgaga ttttccggca
     1621 gtggaaggca ccagtcgttt gtccgccagc ctggcaacgg gcgggttatc gcctcgccaa
     1681 tgtttacatc gcctgttggc ggaacaaccg caggtgctgg aaggcggacc ggggagcgtc
     1741 tggcttaatg agctgatatg gcgcgagttc taccgccatt tgatgacgta ttatccttcg
     1801 ctgtgtaaac atcgtccgtt tattgcctgg acggatcgtg tacagtggca aagcaatccc
     1861 gcccatttaa aggcctggca ggagggcaaa acgggatacc cgattgtcga tgctgccatg
     1921 cgtcagctta acagcactgg ctggatgcat aaccggctac ggatgattac agccagtttt
     1981 ctggtgaaag atttgttgat cgactggcgc gaaggcgagc gatatttcat gtcgcagctg
     2041 attgatggtg atttggcagc taataacggt ggctggcagt gggctgcttc tacgggtact
     2101 gatgcagcgc cgtattttcg tattttcaac ccgacaactc agggcgagaa atttgaccgc
     2161 gaaggcgaat ttatccgcca gtggctaccg gagctgcgcg atgtaccggg gaaagcggtg
     2221 catgagccgt ggaagtgggc agagaaaaca ggtgtgacgt tggattatcc gcaaccgata
     2281 gtcgatcaca aagaggcaag gctgcgaacg ctggcagcgt acgaagaagc gaggaaagga
     2341 gcctgataaa acgcagcgag cgttttatca ggcatggtgc tggcttatta agactccagc
     2401 gccagcgcgc ggtttctgaa cttaagcgcc tgatacagcc aaatcatcag taccagtcct
     2461 acacacgcca gtgcgcccca ggtaatttga tcaaatactt caatatatgc attgatggag
     2521 tagttgatcg ccccggaagc atcaaacgaa gcctgcgatg tctgatcggc aatcacgccc
     2581 gccagatagt tcgcaatcgc gccggaaagc agcatataaa tgccggttaa tacgccggtc
     2641 acgccgggga tttcaatgcg cgtaatctgc gacatagcaa ccggatcgat aaacagttcc
     2701 gcaaagccca tcaccgccag gcctaacacc atcagtggca gagaagagtg accatacatt
     2761 gcggaccagc gggcgcttaa ggtcagaatg cagaatccgg cgctcatcag gccaaggcca
     2821 agagcaaatt ttccccagat gcgcacggta cgattacctg cgacgctttc tttaaccacc
     2881 cacgccagga acacaccgca cagcataact gcgaaggcat taatcgactg gaacatagca
     2941 gtcggaacgg tataaccaaa catatcgcgg ttaacgaagc ggtcgatata aaggctaatc
     3001 gagctacccc cctgttgtgc gaatgcccag aacaacatgc tgaagaaggt gagggtaaca
     3061 atcagcccca gctccttacg ctgtttttgg ttttctgctt tgcgataaat ttttgccaga
     3121 accccaaggc caatgatcgt cgcgacaatc aaggcgtata ccgaccactc tttccagaac
     3181 aacacggtaa tcagcgcagg cgttgccacc agcagaacca gtagccatcc ccagttgggc
     3241 aggagaaagt ttgtcgcacg cagtaccttt ttgttaacgc cgcgggtgtg tgtgaaatga
     3301 cgattgccac ataagaaaat gaccagaccc gcgatcatac ccaccgccgc caggccaaag
     3361 cccatcgccc aactgtactc ttcctgggcg aaaccacagg cgataggtgc gataatagac
     3421 cccacgttac ccgccgcata catcagcgag aaaccgccat cacgacgtgg atcggttggc
     3481 tcatacagct cgccgagcag gcaactgacg ttggatttaa acagaccata gccgcagacg
     3541 ataatcgcca gggacagata gaggaatgac ggatggatct cactggcacc cagcaccaca
     3601 tgaccgatcg ccatcaacaa cgcccccagc atcaccgcca tgcgattgcc gagaacttta
     3661 tccgccaaaa agccaccgag gattggcgtg acatatacca gcgaacagta ggcgctaaat
     3721 aactcgtagg cgtgattatc gctatatttt agttgattgg tgagatagag gatcagcagg
     3781 gcacgcatgc cgtaaaagct aaaatattcc cagatttgca gcgcaacgac atagtaaata
     3841 gcgcgcggtt gtgatgcgtg tttattcata ataattccac aattgactgc aacgtcacgt
     3901 aatagttgcg cggcgtgctt gtgtttcctt aaagttaaaa ctttgataca gatctgatta
     3961 tttatgcaat atgctgtctg attgcataaa tatacattag ctgaagcgtg atgatataaa
     4021 caatttgcgt cttttggcgt cgtgtttgtt tcttaatatg tcaaaagatt ggctatcgac
     4081 gaacaggcga ggtaacgcta tgttaacggt gtagctatca gaatacggaa ggcgcgaaga
     4141 tgaaaaacac cgaactggaa caactgatta acgaaaaact gaacagcgcg gcgattagtg
     4201 attacgcgcc gaatggtttg caggtggaag gcaaagagac ggtgcaaaaa attgttaccg
     4261 gtgtcactgc cagccaggct ttgctcgatg aggcagtgcg tctgggcgcg gatgcggtca
     4321 ttgtgcatca tggctacttc tggaaaggcg aatctccggt cattcgcgga atgaagcgta
     4381 accgcttaaa aacattgctg gcgaatgata tcaacctgta tggctggcat ttgccgcttg
     4441 acgcacatcc tgagctgggc aataacgcac agctggcggc gttactgggg atcacggtca
     4501 tgggcgaaat tgagccgttg gtgccgtggg gcgaactgac catgcctgtg ccgggactgg
     4561 aactggcctc ctggattgaa gcgcgtctgg gacgtaagcc gttatggtgt ggcgataccg
     4621 gacctgaggt ggtccagcgc gtcgcctggt gcacgggcgg cgggcaaagt tttatcgata
     4681 gcgccgcgcg ttttggcgtg gatgctttta ttactggcga agtttctgaa cagaccattc
     4741 attcagcccg cgagcaggga ttgcattttt atgctgcagg tcaccatgcc actgaacgtg
     4801 gtggtattcg cgcattgagc gagtggctga atgaaaatac cgatcttgat gtgaccttta
     4861 ttgatattcc taatcctgca taacgaataa tcagagggat cgaaagtgca acgagcgcgt
     4921 tgttatctga taggtgaaac ggcggtagta ctggaactgg aaccgccggt gacgctggct
     4981 agccagaaac ggatctggcg actggcacag cgtctggtgg atatgccgaa tgtggttgaa
     5041 gccattcctg gcatgaacaa tatcacagtg attttgcgta atcctgagtc gctggcgctg
     5101 gatgccatag agcgtttgca acgctggtgg gaggagagtg aggcgctgga gccggagtct
     5161 cgctttattg aaattccggt ggtttacggt ggcgcagccg gaccggattt ggcggtggtc
     5221 gcggcgcatt gtggattgag cgaaaaacag gttgttgaat tacactcctc cgtggaatac
     5281 gtggtctggt ttttaggttt tcaaccgggc ttcccgtatc tcgggagttt gccggaacaa
     5341 ctacacacgc cacggcgcgc tgaaccgcgc ttgcacgttc cagcaggttc tgtcgggatc
     5401 ggcgggccgc agaccggtgt ttatccgctg gcaacgccgg gtggctggca gttgattggt
     5461 catacctcac tcagcctgtt tgatccggag cgtgacgaac ccatcttatt acgtccggga
     5521 gacagcgtgc gctttgtacc ccagaaggag ggagtatgct gaagattatt cgtgcgggca
     5581 tgtataccac tgtgcaggat ggcggtcgtc acggttttcg ccagtcgggt atcagccact
     5641 gcggcgcact ggatatgcct gcgctgcgca ttgctaacct gctggtgggt aatgacgcca
     5701 acgcccccgc gctggagatc acgctcggtc agttaacggt tgagttcgaa actgatgggt
     5761 ggtttgccct gacgggtgcc ggttgcgaag cgcggctgga tgataacgcc gtctggaccg
     5821 gctggcgatt gccaatgaga gcaggccagc gtttaacgct taaacgcccg cagcacggga
     5881 tgcgcagtta tctggcggtc gcgggtggta ttgatgttcc gccggtaatg ggatcatgca
     5941 gcaccgatct caaagtgggg attggcgggt tggaaggccg tttactgagg gatggtgacc
     6001 gactcccgat tggcaaagcg aagcgtgatt ttatggaagc gcagggcgtt aaacagctgc
     6061 tgtggggcaa ccgcattcgc gccttgccgg ggccggaata tcatgagttc gatcgcgcct
     6121 cgcaggatgc attctggcgt tcgccctggc agcttagctc gcaaagtaac cgcatgggct
     6181 atcgcttaca ggggcaaatt ttaaaacgca ccaccgatcg cgaactgtta tctcacggtt
     6241 tgttaccagg tgtggtgcag gtgccgcata acgggcagcc cattgtgttg atgaacgatg
     6301 cgcaaaccac cggtggttac ccgcgtattg cctgtatcat tgaggctgat atgtaccatc
     6361 tggcgcaaat tccgctcggt cagccgattc attttgtcca gtgttcactg gaagaggcac
     6421 tgaaagcacg gcaagatcaa caacgttatt tcgaacaatt agcgtggcgg ctgcacaatg
     6481 aaaattgacc tgaacgccga tctgggcgaa ggctgcgcca gcgacgcaga actattaacg
     6541 ctggtttcct ctgccaatat tgcctgtgga tttcatgcag gcgatgcgca aaccatgcag
     6601 gcttgcgtgc gtgaagcaat aaaaaacggt gtcgcgattg gcgcgcaccc gagttttccc
     6661 gacagggaaa attttggtcg cagcgccatg cagctgccgc cagaaaccgt tttcgcccag
     6721 acgctgtatc aaattggcgc gctggcagcg attacccgtg cgcaaggcgg cgtaatgtgt
     6781 catgtcaaac cgcacggcat gttatacaac caggcggcga aagaggcaca actggcagac
     6841 gccatcgcca gagcggtata cgcttgcgat ccggcattga ttctcgtcgg gctggcagga
     6901 agcgagctga ttcgtgcagg cgaacgatat ggcctggtaa cgcgcgagga agtgtttgcc
     6961 gatcgcggtt atcaggcaga cggttcgctg gtgccgcgaa gccagccagg cgcgttgatt
     7021 gaaaacgaag aacaggcgct ggcgcaaacg ttggagatgg tgcaatacgg cagagtcaaa
     7081 agtatcaccg gcgaatgggc aatggtcacg gcgcaaacgg tctgcctaca tggcgatggc
     7141 gagcacgcac tggcgttcgc ccgccgatta cgagctacat ttgctgaaaa ggggattgtt
     7201 gtcgcagcat aaccccgctt aataaagaat gaaaaaagga tatcaccatg cctgaaggcc
     7261 cggagatccg ccgtgcagcg gataacctgg aggcggcgat caaaggcaaa ccactaactg
     7321 atgtctggtt tgccttcccg cagttaaaat cttatcaatc acgacttatc ggtcaacacg
     7381 ttacccatgt ggaaacgcgt ggtaaggcgt tgttaactca tttttccaac gacttaacgc
     7441 tctacagcca taatcagctt tacggtgtct ggcgcgtggt tgatacaggc gaagagccgc
     7501 agaccacgcg agtactgcgg gtaaaactgc aaacggctga caaaaccatt ctgctttata
     7561 gcgcctcgga tattgagatg ttgaccccgg aacaactgac cacgcatccg tttttacaac
     7621 gcgttggtcc cgatgtgctg gatccgaatc tgacgccgga ggtggtaaag gaacgattat
     7681 tgtcgccgcg ctttcgtaac cgccagtttg ctggattact gctcgatcag gcgtttctgg
     7741 caggacttgg caattatttg cgggtggaga tcctctggca ggttgggttg accggaaatc
     7801 ataaagcgaa agatctcaat gcggcgcaac tggatgcact cgcacacgcg ttactggata
     7861 ctccgcgact ttcttacgct acgcggggcc aggtggatga aaacaaatat catggggcgc
     7921 tgtttcgctt taaggttttt catcgtgatg gcgaaccttg cgaacgctgt ggcggcatca
     7981 ttgagaaaac cacgctgtca tcgcgcccgt tttactggtg cccaggctgc cagcactagg
     8041 ccgaacgctt cggcgcatag gttgaaataa accgtgcgat ggcaggcccc gtcagcaaaa
     8101 tactgaacag gcgcagggtt tgcatcgcca tgatgagcgc catatcggca ttgctccctg
     8161 cggcgatgac cgccacggta tcaagcccgc cggggctggt ggcgaggtag gcggtcataa
     8221 aatcaatatg cataaaccgg gtcagccccc acgccatacc cgcacaaata gccagcagag
     8281 caaaaatcga cagcaggatt tgtggcagtg ggcgcaatgc ccgcagtaat atttgcttat
     8341 cgaaaccaag accaatccgc cagccaattg ccatatatgc catcgccagc agccattccg
     8401 ggagttcaat cgtgatgagc tgaccagact ggagcactgc gcccgccagc atcgggatga
     8461 gcatcgtgcc ggaaggaaga cgtagcaagc atcccgccgt accggcaacg accgcgagca
     8521 aaatcgtaag caggagatta atgctcactg gcgggaacca gacgatctgc tggttaaccg
     8581 cttcagcgtt atcgcccagc atcatgcggg tgaccagaac agcggccccc gcgacgaaca
     8641 gcactcgcag atattgcata aacgccacca ggcgaatatc tgcgccgtaa tcttgcgcca
     8701 ttgcgaccat tgccgccgcg ccgccaggag aagaacccca ggccccggta tttccaggca
     8761 gtgagctata gcgcaccaat aaccaaccca cgatggcgct ggaaagcagc gtcaccagta
     8821 aaatcgcaag cacgatcggc cagttgacgg ccagcgtggt gagaatcgaa ccagtgaggt
     8881 tttgtgcaat catgcagcca agaatggctt gggcagcgag aaaagcggag cggggaagtt
     8941 gcagagtgac tccgcgcata ctgaagatga tcccggcgat catcgggcca agcaggagtg
     9001 ccgccgggag atgtaaagcg aggaagccaa tagaaaggag aagtgacaga acacataaca
     9061 ttccccactg caatacaggc attcttcgct tcctcattat tttatctttg gtgtatcgat
     9121 acagcataaa gaaagctggg tctggaaaaa ataacggtaa tcaaggaaaa ggggaaaatc
     9181 agcaataatc tgaatacgtt gccctttccg gctgaaaagg gcgtcgtgtt aaatcagtta
     9241 tagttaatct taaaaagtac cactgtatcg aagggacccg ccgcgatggg agtgccaggt
     9301 gtggacgata attcagccgt ataggttttt tcgtagatca gcatatcact ggtgaaatcg
     9361 gcgtattcag catatttatt gaatgtataa ggcgaggcgt tttcatccag tatacgtagc
     9421 ttcagaccat taccaatcaa caatgcttta tcttcttcaa ccaacgtttt ttcggtataa
     9481 aacgaggagc tgaccttaaa tccgtcggta cactggtcat tttgcgattt tgttgtcttg
     9541 atactgaatg ttttcgacat tgtgtggcgg cgaatatcca gtaagttaaa tttacccaaa
     9601 tcaatcacca gtgtttccgg tgaaatattt tttcaccatt attaatgggt atttgcatct
     9661 ggtttaattt ttacaatttt ttatgttcag ccatataaaa agaacccgct ctattaaagg
     9721 cgggtccgga aagtgaacgg cttagcaatc aaccattaac gcttgatatc gcttttaaag
     9781 tcgcgttttt catatcctgt atacagctga cgcggacggg caatcttcat accgtcactg
     9841 tgcatttcgc tccagtgggc gatccagcca accgtacgtg ccatagcgaa gatcacggtg
     9901 aacatagaag acggaatacc catcgctttc aggatgatac cagagtagaa atcgacgttc
     9961 gggtacagtt ttttctcgat aaagtacggg tcgttcagcg cgatgttttc cagctccata
    10021 gccacttcca gcaggtcatc tttggtaccc agctctttca gaacttcatg gcaggtttca
    10081 cgcattacgg tggcgcgcgg gtcgtaattt ttgtacacgc ggtgaccgaa gcccatcagg
    10141 cggaaagaat catttttgtc tttcgcacga cgaacaaatt ctggaatgtg tttaacggag
    10201 ctgatttctt ccagcatttt cagcgccgct tcgttagcac caccgtgcgc aggtccccac
    10261 agtgaagcaa tacctgctgc gatacaggca aacgggttcg cacccgaaga gccagcggta
    10321 cgcacggtgg aggtggaggc gttctgttca tggtcagcgt gcaggatcag aatacggtcc
    10381 atagcacgtt ccagaatcgg attaacttca tacggttcgc acggcgtgga gaacatcata
    10441 ttcaggaagt taccggcgta ggagagatcg ttgcgcgggt aaacaaatgg ctgaccaatg
    10501 gaatacttgt aacacatcgc ggccatggtc ggcatttttg acagcaggcg gaacgcggca
    10561 atttcacgat gacgaggatt gttaacatcc agcgagtcgt gatagaacgc tgccagcgcg
    10621 ccggtaatac cacacatgac tgccattgga tgcgagtcgc gacggaaagc atggaacaga
    10681 cgggtaatct gctcgtggat catggtatga cgggtcaccg tagttttaaa ttcgtcatac
    10741 tgttcctgag tcggtttttc accattcagc aggatgtaac aaacttccag gtagttagaa
    10801 tcggtcgcca gctgatcgat cgggaaaccg cggtgcagca aaataccttc atcaccatca
    10861 ataaaagtaa ttttagattc gcaggatgcg gttgaagtga agcctgggtc aaaggtgaac
    10921 acaccttttg aaccgagagt acggatatca ataacatctt gacccagcgt gcctttcagc
    10981 acatccagtt caacagctgt gtccccgttg agggtgagtt ttgcttttgt atcagccatt
    11041 taaggtctcc ttagcgcctt attgcgtaag actgccggaa cttaaatttg ccttcgcaca
    11101 tcaacctggc tttacccgtt tgttatttgg ctcgccgctc tgtgaaagag gggaaaacct
    11161 gggtacagag ctctgggcgc ttgcaggtaa aggatctatt gatgacgaat aaatggcgaa
    11221 tcaagtactt agcaatccga attattaaac ttgtctacca ctaataactg tcccgaatga
    11281 attggtcaat acttccacac tgttacataa gttaatctta ggtgaaatac cgacttcata
    11341 acttttacgc attatatgct tttcctggta atgtttgtaa caactttgtt gaatgattgt
    11401 caaattagat gattaaaaat taaataaatg ttgttatcgt gacgcagatc actgttcggg
    11461 ataaaacccg acaaactata tgtaggttaa ttgtaatgat tttgtgaaca gcctatactg
    11521 ccgccaggtc tccggaacac cctgcaatcc cgagccaccc agcgttgtaa cgtgtcgttt
    11581 tcgcatctgg aagcagtgtt ttgcatgacg cgcagttata gaaaggacgc tgtctgaccc
    11641 gcaagcagac cggaggaagg aaatcccgac gtctcctggt aacagaaagt taacctctgt
    11701 gcccgtagtc cccagggaat aataagaaca gcatgtgggc gttattcatg ataagaaatg
    11761 tgaaaaaaca aagacctgtt aatctggacc tacagaccat ccggttcccc gtcacggcga
    11821 tagcgtccat tctccatcgc gtttccggtg tgatcacctt tgttgcagtg ggcatcctgc
    11881 tgtggcttct gggtaccagc ctctcttccc ctgaaggttt cgagcaagct tccgcgatta
    11941 tgggcagctt cttcgtcaaa tttatcatgt ggggcatcct taccgctctg gcatatcacg
    12001 tcgtcgtagg tattcgccac atgatgatgg attttggcta tctggaagaa acattcgaag
    12061 cgggtaaacg ctccgccaaa atctcctttg ttattactgt cgtgttttca cttctcgcag
    12121 gagtcctcgt atggtaagca acgcctccgc attaggacgc aatggcgtac atgatttcat
    12181 cctcgttcgt gctaccgcta tcgtcctgac gctctacatc atttatatgg tcggtttttt
    12241 cgctaccagt ggcgagctga catatgaagt ctggatcggt ttcttcgcct ctgcgttcac
    12301 caaagtgttc accctgctgg cgctgttttc tatcttgatc catgcctgga tcggcatgtg
    12361 gcaggtgttg accgactacg ttaaaccgct ggccttgcgc ctgatgctgc aactggtgat
    12421 tgtcgttgca ctggtggttt acgtgattta tggattcgtt gtggtgtggg gtgtgtgatg
    12481 aaattgccag tcagagaatt tgatgcagtt gtgattggtg ctggcggcgc aggtatgcgc
    12541 gcggcgctgc aaatttccca gagtggccag acctgtgcgc tgctctctaa agtcttcccg
    12601 acccgttccc ataccgtttc tgcgcaaggt ggtattaccg ttgcgctggg taatacccat
    12661 gaagataact gggaatggca tatgtacgac accgtaaaag ggtcggacta tatcggtgac
    12721 caggacgcga ttgaatatat gtgtaaaacc gggccggaag cgattctgga actggaacat
    12781 atgggcctgc cgttctcgcg tcttgatgat ggtcgtatct atcaacgtcc gtttggcggt
    12841 cagtcgaaaa acttcggcgg cgagcaggcg gcacgtactg cggcggctgc cgaccgtacc
    12901 ggtcacgcac tgttgcacac cctttatcag cagaacctga aaaaccacac cactattttc
    12961 tccgagtggt atgcgttgga tctggtgaaa aaccaggatg gcgcagtggt cggttgtacc
    13021 gcactgtgca tcgaaactgg tgaagtggtt tactttaaag ctcgcgcgac agtgctggcg
    13081 actggcgggg cagggcgtat ttatcagtcc accactaacg cccacattaa cactggcgac
    13141 ggtgtcggca tggctatccg tgcaggcgta ccggtacagg atatggaaat gtggcagttc
    13201 cacccgaccg gtattgccgg tgcgggcgta ctggtcaccg aaggttgccg tggtgaaggc
    13261 ggttatctgc tgaacaaaca tggcgaacgc tttatggagc gttatgcgcc gaacgccaaa
    13321 gacctggcgg gccgtgacgt ggtagcgcgt tccatcatga tcgaaatccg tgaaggccgc
    13381 ggctgtgatg gtccgtgggg gccacacgca aaactgaaac ttgaccatct gggtaaagaa
    13441 gttcttgaat cccgtctgcc gggtatcctt gaactctccc gcaccttcgc tcacgttgat
    13501 ccggtgaaag agccgattcc ggtaattcca acctgtcact acatgatggg cggtattccg
    13561 accaaagtga ccggtcaggc gctgacggtg aatgagaaag gcgaagatgt ggttgttccg
    13621 gggctgtttg ccgttggtga aatcgcttgt gtatcggtac atggcgctaa ccgtctgggc
    13681 ggcaactcgc tgctggacct ggtggtcttt ggtcgtgcgg caggtctgca tctgcaagag
    13741 tctatcgccg agcagggcgc actgcgcgat gccagcgagt ctgatgttga agcgtctctg
    13801 gatcgcctga accgctggaa caataaccgt aacggtgaag atccggtggc gatccgtaaa
    13861 gcactgcaag aatgtatgca gcataacttc tcggtcttcc gtgaaggtga tgcgatggcg
    13921 aaagggcttg agcagttgaa agttatccgc gagcgtctga aaaatgcccg tctggatgac
    13981 acttccagcg agttcaacac ccagcgcgtt gagtgcctgg aactggataa cctgatggaa
    14041 acggcgtatg caacggctgt ttctgccaac ttccgtaccg aaagccgtgg cgcgcatagc
    14101 cgcttcgact tcccggatcg cgatgatgaa aactggctgt gccactccct gtatctgcca
    14161 gagtcggaat ccatgacgcg ccgaagcgtc aacatggaac cgaaactgcg cccggcattc
    14221 ccgccgaaga ttcgtactta ctaatgcgga gacaggaaaa tgagactcga gttttcaatt
    14281 tatcgctata acccggatgt tgatgatgct ccgcgtatgc aggattacac cctggaagcg
    14341 gaagaaggtc gtgacatgat gctgctggat gcgcttatcc agctgaaaga gaaagatccc
    14401 agcctgtcgt tccgccgctc ctgccgtgaa ggtgtgtgcg gttccgacgg tctgaacatg
    14461 aacggtaaga atggtctggc ctgtattacc ccgatttcgg cactcaacca gccgggcaag
    14521 aagattgtga ttcgtccgct gccaggttta ccggtgatcc gcgatttggt ggtagacatg
    14581 ggacaattct atgcgcaata tgagaaaatt aagccttacc tgttgaataa tggacaaaat
    14641 ccgccagctc gcgagcattt acagatgcca gagcagcgcg aaaaactcga cgggttgtat
    14701 gaatgtattc tctgcgcatg ttgttcaacc tcttgtccgt ctttctggtg gaatcccgat
    14761 aagtttatcg gtccggcagg cttgttagcg gcatatcgtt tcctgatcga tagccgtgat
    14821 accgagactg acagccgcct cgacggtttg agcgatgcat tcagtgtatt ccgctgtcac
    14881 agcatcatga actgcgtcag tgtatgtccg aaggggctga acccgacgcg cgccatcggc
    14941 catatcaagt cgatgttgtt gcaacgtaat gcgtaaaccc aggcctgata agacgcacaa
    15001 gcgtcgcatc aggcaaccag tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag
    15061 aaatgcagaa accgtaggcc tgataagacg cgcaagcgtc gcatcaggca accagtgccg
    15121 gatgcggcgt gaacacctta tccggcctac aaaagaaatg cagaaaccgt aggcctgata
    15181 agacgcacaa gcgtcgcatc aggcaaacag cgccggatgc ggcgtgaatg ccttatccgg
    15241 cctacaaaag aaatgcagga aatctttaaa aactgcccct gacactaaga cagtttttaa
    15301 aggttcctta gcggacgcag aaaacacgac aagtccgcaa caagtgaacc ccggcacgca
    15361 catcagatat gaaatgtgcg tggtagtatc cacggcgaag taagcataaa aaagatgctt
    15421 aagggatcac gatgcagaac agcgctttga aagcctggtt ggactcttct tacctctctg
    15481 gcgcaaacca gagctggata gaacagctct atgaagactt cttaaccgat cctgactcgg
    15541 ttgacgctaa ctggcgttcg acgttccagc agttacctgg tacgggagtc aaaccggatc
    15601 aattccactc tcaaacgcgt gaatatttcc gccgcctggc gaaagacgct tcacgttact
    15661 cttcaacgat ctccgaccct gacaccaatg tgaagcaggt taaagtcctg cagctcatta
    15721 acgcataccg cttccgtggt caccagcatg cgaatctcga tccgctggga ctgtggcagc
    15781 aagagaaagt ggccgatctg gatccgtctt tccacgatct gaccgaagca gacttccagg
    15841 agaccttcaa cgtcggttca tttgccagcg gcaaagaaac catgaaactc ggcgagctgc
    15901 tggaagccct caagcaaacc tactgcggcc cgattggtgc cgagtatatg cacattacca
    15961 gcaccgaaga aaaacgctgg atccaacagc gtatcgagtc tggtcgcgcg actttcaata
    16021 gcgaagagaa aaaacgcttc ttaagcgaac tgaccgccgc tgaaggcctt gaacgttacc
    16081 ttggcgcaaa attccctggc gcaaaacgct tctcgctgga aggtggtgac gcgttaatcc
    16141 cgatgcttaa agagatgatc cgccacgctg gcaacagcgg cacccgtgaa gtggttctcg
    16201 ggatggcgca ccgtggtcgt ctgaacgtgc tggtgaacgt gctgggtaaa aaaccgcaag
    16261 acttgttcga cgagtttgcc ggtaaacata aagaacacct cggcacgggc gacgtgaaat
    16321 accacatggg cttctcgtct gacttccaga ccgatggcgg cctggttcac ctggcgctgg
    16381 cgtttaaccc gtctcacctt gagattgtaa gcccggtcgt tatcggttct gttcgtgccc
    16441 gtctggacag actcgacgag ccgagcagca acaaagtgct gccaatcacc attcatggtg
    16501 acgccgcagt gaccgggcag ggcgtggttc aggaaaccct gaacatgtcg aaagcgcgtg
    16561 gttatgaagt tggcggtacg gtacgtatcg ttatcaacaa ccaggttggc ttcaccacct
    16621 ctaacccgct ggatgcccgt tcgacgccgt actgtactga tatcggtaag atggttcagg
    16681 caccgatttt ccacgttaac gcggatgatc cggaagccgt tgcctttgtt acccgtctgg
    16741 cgctcgattt ccgtaacacc tttaaacgtg atgtcttcat cgacctggta tgctaccgcc
    16801 gtcacggcca caacgaagcc gacgagccga gcgcaaccca gccgctgatg tatcagaaaa
    16861 tcaaaaaaca tccgacgccg cgcaaaatct acgctgacaa gctggagcag gaaaaagtcg
    16921 cgacgctgga agatgccacc gagatggtta acctgtaccg cgatgcgctg gatgctggcg
    16981 attgcgttgt agcagagtgg cgtccgatga acatgcactc tttcacctgg tcgccgtacc
    17041 tcaaccacga atgggacgaa gagtacccga acaaagttga gatgaagcgc ctgcaggaac
    17101 tggctaaacg catcagcacg gtgccggaag cggttgaaat gcagtctcgc gttgccaaga
    17161 tttatggcga tcgccaggca atggctgccg gtgagaaact gttcgactgg ggcggcgcgg
    17221 aaaacctcgc ttacgccacg ttggttgacg aaggcattcc ggttcgcctg tcgggtgaag
    17281 actccggtcg cggtaccttc ttccaccgcc acgcggtgat ccacaaccag tctaacggtt
    17341 ccacttacac gccgctgcaa catatccata acggccaggg cgcgttccgc gtctgggact
    17401 ctgtactgtc tgaagaagcc gtactggcgt ttgaatacgg ttatgccacc gcagaaccac
    17461 gcaccctgac catctgggaa gcacagttcg gtgacttcgc caacggtgca caggtggtta
    17521 tcgaccagtt tatctcctct ggcgaacaga aatggggccg gatgtgtggc ctggtgatgt
    17581 tgctgccgca cggttacgaa gggcaggggc cggagcactc ctccgcgcgt ctggaacgtt
    17641 atctgcagct ttgcgctgag caaaacatgc aggtttgcgt accttctacc ccggcacagg
    17701 tttaccacat gctgcgtcgt caggcgttgc gcgggatgcg tcgtccactg gtcgtgatgt
    17761 cgccgaaatc cctgctgcgt catccgctgg cggtatccag cctcgaagaa ctggcgaacg
    17821 gcaccttcct gccagccatc ggtgaaatcg acgagcttga tccgaagggc gtgaagcgcg
    17881 tagtgatgtg ttctggtaag gtttattacg acctgctgga acaacgtcgt aagaacaatc
    17941 aacacgatgt cgccattgtg cgtatcgagc aactctaccc gttcccgcat aaagcgatgc
    18001 aggaagtgtt gcagcagttt gctcacgtca aggattttgt ctggtgccag gaagagccgc
    18061 tcaaccaggg cgcatggtac tgcagccagc atcatttccg tgaagtgatt ccgtttgggg
    18121 cttctctgcg ttatgcaggc cgcccagcct ccgcctctcc ggcggtaggg tatatgtccg
    18181 ttcaccagaa acagcaacaa gatctggtta atgacgcgct gaacgtcgaa taaataaagg
    18241 atacacaatg agtagcgtag atattctggt ccctgacctg cctgaatccg tagccgatgc
    18301 caccgtcgca acctggcata aaaaacccgg cgacgcagtc gtacgtgatg aagtgctggt
    18361 agaaatcgaa actgacaaag tggtactgga agtaccggca tcagcagacg gcattctgga
    18421 tgcggttctg gaagatgaag gtacaacggt aacgtctcgt cagatccttg gtcgcctgcg
    18481 tgaaggcaac agcgcgggta aagaaaccag cgcgaaatct gaagagaaag cgtccactcc
    18541 ggcgcaacgc cagcaggcgt ctctggaaga gcaaaacaac gatgcgttaa gcccggcgat
    18601 ccgtcgcctg ctggctgaac ataacctcga cgccagcgcc attaaaggca ccggtgtggg
    18661 tggtcgtctg acccgtgaag atgtggaaaa acatctggcg aaagccccgg cgaaagagtc
    18721 tgctccggca gcggctgctc cggcggcgca accgaccctg gctgcacgta gcgaaaaacg
    18781 tgtgccgatg actcgcctgc gtaagcgtgt ggcagagcgt ctgctggaag cgaaaaactc
    18841 caccgccatg ctgaccacgt tcaacgaagt caacatgaag ccgattatgg atctgcgtaa
    18901 gcagtacggt gaagcgtttg aaaaacgcca cggcattcgt ctgggcttta tgtccttcta
    18961 cgtgaaagcg gtggttgaag ccctgaaacg ttacccggaa gtgaatgcgt ctatcgacgg
    19021 cgatgatgtg gtctaccaca actatttcga cgtcagtatg gcggtttcta cgccgcgcgg
    19081 cctggtgacg ccggttctgc gtgatgtcga taccctcggc atggcagaca tcgagaagaa
    19141 aatcaaagag ctggctgtta aaggccgtga cggcaagctg acggttgaag atctgaccgg
    19201 tggtaacttc accatcacca acggtggtgt gttcggttcc ctgatgtcaa cgcctatcat
    19261 caacccaccg cagagcgcaa ttctgggtat gcacgctatc aaagatcgtc cgatggcagt
    19321 gaatggccag gttgagatcc tgccgatgat gtacctggcg ctgtcctacg atcaccgttt
    19381 gatcgatggt cgcgaatccg tgggcttcct ggtaacgatc aaagagttgc tggaagatcc
    19441 gacgcgtctg ctgctggacg tttagtagtt taagcttcac ctgcactgta gaccgacgtg
    19501 gatagggtgt cggtctacgg tttaaaagat aacgattact gaaggatgga cagaacacat
    19561 gaacttacat gaatatcagg caaaacaact ttttgcccgc tatggcttac cagcaccggt
    19621 gggttatgcc tgtactactc cgcgcgaagc agaagaagcc gcttcaaaaa tcggtgccgg
    19681 tccgtgggta gtgaaatgtc aggttcacgc tggtggccgc ggtaaagcgg gcggtgtgaa
    19741 agttgtaaac agcaaagaag acatccgtgc ttttgcagaa aactggctgg gcaagcgtct
    19801 ggtaacgtat caaacagatg ctaatggcca accggttaac cagattctgg ttgaagcagc
    19861 gaccgatatc gctaaagagc tgtatctcgg tgccgttgtt gaccgtagtt cccgtcgcgt
    19921 ggtctttatg gcctccaccg aaggcggcgt ggaaatcgaa aaagtggcgg aagaaactcc
    19981 gcacctgatc cacaaagttg cgcttgatcc gctgactggc ccgatgccgt atcagggacg
    20041 cgaactggcg tttaaactgg gtctggaagg taaactggtt cagcagttca ccaaaatctt
    20101 catgggcctg gcgaccattt tcctggagcg cgaccttgcg cttatcgaaa tcaacccgct
    20161 ggttattacc aaacagggcg atctgatttg cctcgacggc aaactgggcg ctgatggcaa
    20221 cgcactgttc cgccagcctg atctgcgtga aatgcgtgac cagtcgcagg aagatccgcg
    20281 tgaagcacag gctgcacagt gggaactgaa ctacgttgcg ctggacggta acatcggttg
    20341 tatggttaac ggcgcaggtc tggcgatggg tacgatggac atcgttaaac tgcacggcgg
    20401 cgaaccggct aacttcctgg acgttggcgg cggcgcaacc aaagaacgtg taaccgaagc
    20461 gttcaaaatc atcctctctg acgacaaagt gaaagccgtt ctggttaaca tcttcggcgg
    20521 tatcgttcgt tgcgacctga tcgctgacgg tatcatcggc gcggtagcag aagtgggtgt
    20581 taacgtaccg gtcgtggtac gtctggaagg taacaacgcc gaactcggcg cgaagaaact
    20641 ggctgacagc ggcctgaata ttattgcagc aaaaggtctg acggatgcag ctcagcaggt
    20701 tgttgccgca gtggagggga aataatgtcc attttaattg ataaaaacac caaggttatc
    20761 tgccagggct ttaccggtag ccaggggact ttccactcag aacaggccat tgcatacggc
    20821 actaaaatgg ttggcggcgt aaccccaggt aaaggcggta ctacccacct cggcctgcca
    20881 gtgttcaaca ccgtgcgtga agctgttgct gctactggcg ctaccgcctc tgttatctac
    20941 gtaccagcac cgttctgcaa agactccatt ctggaagcta tcgatgcagg catcaaactg
    21001 attatcacca tcactgaagg catcccgacg ctggatatgc tgacggtgaa agtgaaactg
    21061 gatgaagcag gcgttcgtat gatcggcccg aactgcccag gcgttatcac cccgggggaa
    21121 tgcaaaatcg gtatccagcc gggtcacatt cacaaaccgg gtaaagtggg tatcgtttcc
    21181 cgttccggta cgctgaccta tgaagcggtt aaacagacca cggattacgg tttcggtcag
    21241 tcgacctgtg ttggtatcgg cggcgatccg atccctggct ctaactttat cgacatcctc
    21301 gaaatgttcg aaaaagatcc gcagaccgaa gcgatcgtga tgatcggtga gatcggcggt
    21361 agcgctgaag aagaagcagc ggcgtacatc aaagagcacg ttaccaagcc agttgtgggt
    21421 tacatcgctg gtgtgactgc gccgaaaggc aaacgtatgg gtcacgcagg tgcgatcatt
    21481 gccggtggga aagggactgc tgatgagaaa ttcgctgctc tggaagccgc aggcgtgaaa
    21541 accgttcgca gcctggcgga tatcggtgaa gcactgaaaa ctgttctgaa ataaatatct
    21601 gtaataagaa atagccctcg ccgcttccct ctacaggagg ggcgaagggc tgtcggtttc
    21661 gacatggttg gccatcttat gatggccttt tttatgctta tcgcgatgat tttcgctgcg
    21721 ctatcagggt aaatttatag tcatcggtat taaaagcgtt gcggctatat tcaaacaccc
    21781 ggccatcaac taaatatcca cgcgatactt tttcaagaat cggcttagtc tggctgatat
    21841 taagcagacg gctcatttct tcggttggca tcagaggaat gatttcctgt tcgctacgat
    21901 cgataaccat tttcttcact tcttcaataa attgatattt cgaattttcc ataaactgcc
    21961 aggtgagatc cgggaacaac gcaagcggca tccaggtttc ttccagcgcc attggctttt
    22021 gcttgcgata gcgcacgcgc ttcacatgcc acacacgatc ctgtggggtg atttgtagct
    22081 gttgctgaag aaaatcgtca gccggaatca cttcgaatat cagaacttca ctgtgtgtat
    22141 cgacgtgacg gtccgtcagt ttttcatcaa aactggttaa ctgaaaaatg tcgtaattga
    22201 cccgctcttc ttttacgtaa gtcccgctgc cctgaatact ttcgaggatc tgctgttcaa
    22261 ctaactggcg caaagcctga cgcaccgtaa cccggctgac gccaaactct gtttgtagcg
    22321 ctgattcagt gggtaacgca tcgccaggtt taagctcgcc acgcgcaatt tgctcacgaa
    22381 tgcgatcagc aatctgtcgg tataagggtt tccgtcccat gatttagcat ctcattaata
    22441 cgaatttagc cattatgccc gataaattca tcctgtaaat aatacaaata caatacaaat
    22501 aatttcaatc aagtgaaatt gatcacataa tggtgttgtt ttatcgggca cactggcgcg
    22561 actataaaaa cgatcaagtg aggatcatga tgaacctgac gactctgacc caccgcgatg
    22621 cgttgtgtct gaatgcgcgc tttaccagcc gtgaagaggc catccacgcg ttgactcaac
    22681 gtcttgctgc tctggggaaa atttccagta ctgagcaatt tctgaaagaa gtgtatcgcc
    22741 gtgaaagcct tggcccgacc gccttaggtg aagggctggc tgtgccgcat ggcaaaactg
    22801 ctgcggtaaa agaagcggcg tttgcggtcg ccacactcag cgagccgctt cagtgggaag
    22861 gcgtggatgg cccggaagcg gttgatttag tggtgttgct ggcgattccc cccaatgaag
    22921 cgggtactac gcacatgcaa ctgctgacag cgctgaccac gcgcctggcg gatgatgaga
    22981 tacgggcgcg tatacagtcg gcgacgacgc ctgatgaatt gctctcggcg ctggatgaca
    23041 agggaggcac gcaaccctca gcttcttttt ccaacgcgcc aactatcgtc tgcgtaacgg
    23101 cctgcccggc gggtattgct cacacataca tggctgcgga gtatctggaa aaagccggac
    23161 gcaaactcgg cgtgaatgtc tacgttgaaa agcaaggtgc taacggcatt gaagggcgtt
    23221 taacggcaga gcaactcaat agtgcaaccg cctgtatttt tgcggctgaa gtcgccatca
    23281 aggagagtga gcgttttaac ggcattcccg cgctttctgt gcctgttgcc gagccgattc
    23341 gccatgcgga agcgttgatc caacaagcac tgaccctcga gcgtagcggt gagacgcgta
    23401 ccgtacagca agatacgcaa ccggcgaaaa gcgtcaaaac ggagctgaaa caggcgctgt
    23461 taagcgggat ctctttcgcc gtgccgttga ttgtcgcggg ggggacggtg ctggcggtcg
    23521 cggtattact gtcgcaaatc ttcgggctac aagatttgtt taatgaagaa aactcctggc
    23581 tgtggatgta ccgcaaactg ggcggagggc tgctcgggat tttgatggta ccggtgctgg
    23641 cagcctacac cgcctattca ctggctgata aaccggcgtt agcgccaggc tttgcggctg
    23701 gacttgccgc caacatgatc ggttccgggt ttctcggtgc tgtcgttggc ggattgatag
    23761 ccggttatct gatgcgctgg gtgaaaaatc acttgcgtct tagcagtaaa ttcaatggat
    23821 tcctgacttt ttatctctac ccggtgctcg gtacgttggg agcgggcagt ctgatgctgt
    23881 ttgtggtggg ggaacctgtc gcctggatca ataactcgct taccgcctgg ctgaacggtc
    23941 tgtcaggaag taacgcgctg ttgctgggtg ccattctcgg ttttatgtgt tcctttgacc
    24001 ttggagggcc agtgaacaaa gccgcttatg cattctgcct gggcgcaatg gcgaacggcg
    24061 tttacggccc gtatgccatt ttcgcctccg tcaaaatggt ttcggcattt accgtaaccg
    24121 cttccacgat gctcgcaccg cgcctgttta aagagtttga aattgagacc gggaaatcca
    24181 cctggctgtt agggctggct ggtattaccg aaggggcgat cccgatggcg attgaagatc
    24241 cgctgcgggt tattggttcg tttgtgctgg gctctatggt aacgggcgct atcgtcggcg
    24301 cgatgaatat cggcctttcg acacccggtg ccggcatttt ctcgctcttt ttacttcatg
    24361 ataatggcgc gggcggtgtt ttggcggcaa ttggctggtt tggtgcggca ttggtggggg
    24421 ctgcaatctc gactgcaatt ctcctgatct ggcggcgtca cgcggttaag catggcaact
    24481 atctgactga tggcgtaatg ccataaacaa aaaggaaacg acgatgaaag cggtatctcg
    24541 cgttcacatc accccgcata tgcactggga tcgggagtgg tatttcacca ccgaagagtc
    24601 acgcattctg ctggtcaata atatggaaga gatcctgtac cgacttgaac aggacaacga
    24661 atacaaatat tacgtactcg acgggcaaac ggcgatcctc gaagattatt tcgcggtgaa
    24721 accggaaaac aaagaccgtg tgaagaaaca ggtagaagca ggcaagttga ttatcggccc
    24781 ctggtatacc cagaccgata ccacgattgt ttctgcggaa tccatcgtcc gtaatctgat
    24841 gtacggaatg cgtgactgcc tcgcgtttgg cgagccgatg aaaataggtt atttaccaga
    24901 ttcctttggc atgtccgggc aactgccgca tatttacaat ggatttggca ttacccgcac
    24961 catgttctgg cgcggatgtt ctgagcgcca cggtactgat aaaaccgagt ttttgtggca
    25021 aagcagtgac ggtagcgaag tgacggcgca ggtgctgccg ctgggctacg ccatcggtaa
    25081 gtacttacct gccgacgaag acggattacg caaacgcctc gacagttatt ttgatgtgct
    25141 ggaaaaagcg tcagtaacca aagaaatttt attgccgaat ggtcatgacc agatgccatt
    25201 gcagcaaaat atcttcgaag tgatggataa gctacgtgag atctaccctc aacgtaagtt
    25261 tgtgatgagc cgctttgaag aggtatttga gaagatcgaa gcgcagcgag ataatctggc
    25321 aaccctgaaa ggggaattta ttgatggcaa atatatgcgc gtgcatcgca ccatcggttc
    25381 tacgcgtatg gatatcaaaa ttgcccacgc gcgtattgaa aataagattg ttaatctgct
    25441 ggaaccgctg gcaacactgg cctggacgtt gggttttgaa taccaccacg gcttactgga
    25501 gaaaatgtgg aaagagatct taaaaaatca tgcccacgac agtatcggtt gttgttgcag
    25561 tgacaaagtt catcgcgaaa tcgtcgcccg cttcgaactg gctgaagaca tggcggataa
    25621 tctactgcgt ttctacatgc gcaaaattgc cgataacatg ccacagagtg atgccgacaa
    25681 actcgtgctg tttaacctga tgccctggcc gcgggaagaa gtgatcaaca ccactgtgcg
    25741 gctgcgcgct agccagttta atttgcggga cgatcgcggt cagcctgtac cgtattttat
    25801 tcgccatgcc cgtgagatcg atcctggcct aatcgatcgg caaatcgttc attacggtaa
    25861 ttacgatccc tttatggagt ttgatattca gatcaaccag attgtccctt ctatgggcta
    25921 tcgcacgctt tatatcgaag cgaatcagcc tggcaacgtg attgcggcaa aaagtgacgc
    25981 tgaagggata ctggaaaatg ctttctggca aattgcgctc aatgaggatg gaacgctaca
    26041 actggtagat aaagacagcg gtgtgcgcta tgaccgggtg ttgcaaattg aagaaagctc
    26101 tgatgatggt gatgaatatg actattcacc cgcaaaagaa gagtgggtaa ttacttcggc
    26161 gaacgcgaaa ccgcaatgcg agattactca tgaggcctgg cagagtaggg ctgttatccg
    26221 ctatgacatg gcggtgccgc gtaatctgca tgaacgcagc gcaagacaat ccactggcag
    26281 agtaggggtg gagttggctg tcactctgag tgataacagc aggcgcattg atgtggatat
    26341 caaccttgat aaccaggctg acgatcatcg tcttcgcgtc ctggtcccta caccttttaa
    26401 caccgacagt gttctggccg atacccagtt tggttcgcta acgcgccccg tgaacaacag
    26461 tgcaatgaac aactggcagc aagaaggctg gaaagaagcg ccggttccgg tatggaatat
    26521 gctcaactat gctgccttac aggaagggcg taacggtctg gctgtcttta gcgaagggct
    26581 acgtgaattt gaagtcatcg gtgaagagaa gaaaaccttt gccattacgt tgctgcgtgg
    26641 cgtgggctta ctggggaaag aagatctgct tttaaggcct ggacgtcctt caggaataaa
    26701 aatgccggtg cccgattcac aattacgtgg ctcctttagc tgccgtctga gcttatttag
    26761 ttatacaggt acgccttcga ccgccggtgt agcccagcag gcgcgcgcat ggctgacgcc
    26821 agtacagtgt tacaacaaaa tcccgtggga tgcgatgaag ttaaataaag ccggattcaa
    26881 tgtgccggaa agctatagct tgctgaaaat gccccctgtg ggatgcctga ttagcgctct
    26941 taaaaaagcg gaagaccggc aagaagtggt attgcgactg tttaatccgg cagaatcgac
    27001 aacctgtgag gcaactgtcg cgttcagtcg tgaggtgatt tcatgtacgc aaacgatgat
    27061 ggatgaaagc atcagcacag cggaaagaga taatttaaag gaactggggg catttttacc
    27121 ggggcagtca cggacattta gctaccgcct tggctgatta ttgcgctaaa agtgtatcta
    27181 aggggggcaa taatttcagg ccatcttcgg atggcctgag attgttgata aacaagtttg
    27241 ttagcttacg cagaaaccag atttgacctg cgtgagaacc gcaggccatt tatcatttca
    27301 gttacccctt aaacgttaac cacggtcgaa atctgaccgc tggctgaatt aatccggctt
    27361 cttgttgggc ttcgataacc ggaccaatat ctttataagc cgctggcgct tcttcaaaac
    27421 gtcgttcttc gcgcaatgtg acgcattgcc acggcagcgt tgactcttca gtcgcgcgtg
    27481 agcgcatggc ctgccgacgc tggctgcgtc ctgctccgtg actgcatgac cacagccact
    27541 ccgggttgcc gcaacctacc gcaagccacg aataatcgcc cattgaaccg gggattaacg
    27601 ccagattgcc cactcgtgcc ggtgttgcgc ctttgcgatg aatattcatt tcatgctcgc
    27661 gaaggatgat gttatgtggc acatccacca ccagatgcga attgtcctga tgaaagagat
    27721 tggcaagaca tacgcgcacc atttcggcaa ttgtgacgcg atttaaccat gcgtagcgcg
    27781 ccgccattcc tatcgcttgt agatattcct ccgccagtcc gcctgccaga ccatataacc
    27841 cgctttgtgg atgtttatgt ccggtgggcc attcagcacg ggcgcgatcc atccagcgtc
    27901 ttccgacgta aaagccaaca tctcgcgatc cactgtggat catcaccaca atatcgcctg
    27961 ttttcagccc ttgttgccag gctacatggc gatcaaccac gctatcaacg acctgaagtt
    28021 caataaaatg attacctgaa cccggcgtac ccagacaagg atcgcggagt aatgcgcggt
    28081 ccgttgccag cagggcgggt ggggcataac gcgaatgact gttatgctcg ttaagcccaa
    28141 tgcagcggtc aatctcttgt accattcggg ggatattcgt gcgtgtccag agacctgctg
    28201 atggcaaggc tgacatgcaa tctgccggtc cgccatcaaa aagcgcggtg aacgctcgtg
    28261 cggataacgg aacatcgcgc tcattttgca gtaacacttg tgttaaccgc tgagtgatta
    28321 tttctttatt cgcttcggct tgctcaaggg ttaatccggt cgtcagcagc cgcatgccac
    28381 aagaaatatc ggtgccgatg gcggcaggaa tgactaaatc cggcgtagag gcgaccaccg
    28441 cgcctacggg gacgccattg ccggggtgaa aatctggcgt cgcacaggtc tggcgtacat
    28501 aacctggatg atctggtagc ttcacttctg caaaatcaag aagttgtgtc actgcttttt
    28561 cttctagcgg caactcgtcc ggcaacagta cacgggctct ggctcctgat ggacgagata
    28621 atgaaacaat attgttggcg cgggaaagtt ggatcccttt gcgcagaaag gctttatcaa
    28681 gcctcgaaag atgatgcata acgctgtcct gaaatataga cactaaacac ccccctttat
    28741 tgcgagggta attttgtctt tccggtcagc agccagcaaa agggaaagaa aacgcgcgta
    28801 gtgtatgcag caatgacgat gaatacaaga taaaaatgcc gattaacaaa agggggttgt
    28861 tgttataaag
//
LOCUS       DAZFJL010000052        28442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFJL010000052 DAZFJL010000000
VERSION     DAZFJL010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.052"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..208
                     /locus_tag="ACHJ8C_002194"
     CDS             <1..208
                     /locus_tag="ACHJ8C_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5552046.1"
                     /translation="DHFIGPITIAGIVLLIIVAKFTGNLKLGSGEKSNRKTGNRNAHS
                     RSSSRNSFRGGGGSSGGGGSSGRW"
     gene            214..1086
                     /locus_tag="ACHJ8C_002195"
     CDS             214..1086
                     /locus_tag="ACHJ8C_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO5552047.1"
                     /translation="MRLIFILFTLWCLPGLAQQIAVPELRQQVTDITGTLSTSEQQSL
                     TQQLQDITHKTRAQVAVLVVPSTGDDSIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGNIIRSIMIPAFKKQKLAKGLELAIIALNNQLTSQHQY
                     SSNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGNNFCRAFKSSVCISTVYLIDL
                     FLFSDQTFSIAVFSFFFAFTIIMVFTWLCVPQKKASGRSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1146..2444)
                     /gene="eno"
                     /locus_tag="ACHJ8C_002196"
     CDS             complement(1146..2444)
                     /gene="eno"
                     /locus_tag="ACHJ8C_002196"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO5552048.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2532..4169)
                     /gene="pyrG"
                     /locus_tag="ACHJ8C_002197"
     CDS             complement(2532..4169)
                     /gene="pyrG"
                     /locus_tag="ACHJ8C_002197"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO5552049.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4397..5188)
                     /gene="mazG"
                     /locus_tag="ACHJ8C_002198"
     CDS             complement(4397..5188)
                     /gene="mazG"
                     /locus_tag="ACHJ8C_002198"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO5552050.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5260..5595)
                     /gene="mazF"
                     /locus_tag="ACHJ8C_002199"
     CDS             complement(5260..5595)
                     /gene="mazF"
                     /locus_tag="ACHJ8C_002199"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO5552051.1"
                     /translation="MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(5595..5843)
                     /gene="mazE"
                     /locus_tag="ACHJ8C_002200"
     CDS             complement(5595..5843)
                     /gene="mazE"
                     /locus_tag="ACHJ8C_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO5552052.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5921..8155)
                     /gene="relA"
                     /locus_tag="ACHJ8C_002201"
     CDS             complement(5921..8155)
                     /gene="relA"
                     /locus_tag="ACHJ8C_002201"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO5552053.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(8203..9504)
                     /gene="rlmD"
                     /locus_tag="ACHJ8C_002202"
     CDS             complement(8203..9504)
                     /gene="rlmD"
                     /locus_tag="ACHJ8C_002202"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO5552054.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LECFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9561..12317
                     /gene="barA"
                     /locus_tag="ACHJ8C_002203"
     CDS             9561..12317
                     /gene="barA"
                     /locus_tag="ACHJ8C_002203"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO5552055.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSLPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12548..13888)
                     /gene="gudD"
                     /locus_tag="ACHJ8C_002204"
     CDS             complement(12548..13888)
                     /gene="gudD"
                     /locus_tag="ACHJ8C_002204"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO5552056.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGTYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPLEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13909..15249)
                     /gene="gudX"
                     /locus_tag="ACHJ8C_002205"
     CDS             complement(13909..15249)
                     /gene="gudX"
                     /locus_tag="ACHJ8C_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO5552057.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVEAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGQALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15251..16603)
                     /gene="gudP"
                     /locus_tag="ACHJ8C_002206"
     CDS             complement(15251..16603)
                     /gene="gudP"
                     /locus_tag="ACHJ8C_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO5552058.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(17037..17486)
                     /gene="yqcA"
                     /locus_tag="ACHJ8C_002207"
     CDS             complement(17037..17486)
                     /gene="yqcA"
                     /locus_tag="ACHJ8C_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO5552059.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTMLS"
     gene            complement(17504..18286)
                     /gene="truC"
                     /locus_tag="ACHJ8C_002208"
     CDS             complement(17504..18286)
                     /gene="truC"
                     /locus_tag="ACHJ8C_002208"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO5552060.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMLVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18286..18615)
                     /gene="yqcC"
                     /locus_tag="ACHJ8C_002209"
     CDS             complement(18286..18615)
                     /gene="yqcC"
                     /locus_tag="ACHJ8C_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO5552061.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPLPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNNQPLPGAFAVAPYYEMALVTDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19237..19782)
                     /gene="syd"
                     /locus_tag="ACHJ8C_002210"
     CDS             complement(19237..19782)
                     /gene="syd"
                     /locus_tag="ACHJ8C_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO5552062.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19850..20698
                     /gene="queF"
                     /locus_tag="ACHJ8C_002211"
     CDS             19850..20698
                     /gene="queF"
                     /locus_tag="ACHJ8C_002211"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO5552063.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVCQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNNDFVPST
                     TRLVRQ"
     gene            20810..22174
                     /gene="ppnN"
                     /locus_tag="ACHJ8C_002212"
     CDS             20810..22174
                     /gene="ppnN"
                     /locus_tag="ACHJ8C_002212"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO5552064.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            22731..24020
                     /gene="sdaC"
                     /locus_tag="ACHJ8C_002213"
     CDS             22731..24020
                     /gene="sdaC"
                     /locus_tag="ACHJ8C_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO5552065.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPIIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            24078..25445
                     /gene="sdaB"
                     /locus_tag="ACHJ8C_002214"
     CDS             24078..25445
                     /gene="sdaB"
                     /locus_tag="ACHJ8C_002214"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO5552066.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            25557..26312
                     /gene="xni"
                     /locus_tag="ACHJ8C_002215"
     CDS             25557..26312
                     /gene="xni"
                     /locus_tag="ACHJ8C_002215"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO5552067.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHNEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ENHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(26417..27565)
                     /gene="fucO"
                     /locus_tag="ACHJ8C_002216"
     CDS             complement(26417..27565)
                     /gene="fucO"
                     /locus_tag="ACHJ8C_002216"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO5552068.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVIDKMDAAGLAWAIYDGVVPNPTITVVKEGLDVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(27593..28240)
                     /gene="fucA"
                     /locus_tag="ACHJ8C_002217"
     CDS             complement(27593..28240)
                     /gene="fucA"
                     /locus_tag="ACHJ8C_002217"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO5552069.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EANLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         6906 a         7595 c         6813 g         7128 t
ORIGIN      
        1 tgatcatttt atcggcccaa taaccattgc tggcatcgtt ctgctgatca ttgtggccaa
       61 atttactggc aatcttaagc tggggtcagg agaaaaaagc aacaggaaaa cggggaatcg
      121 taatgctcat tctcgctctt cgtcgagaaa ttctttccgc ggtggtggcg gttcttcagg
      181 aggcggcggt tcatccggcc gttggtgatc gtaatgcgct taatctttat tctattcact
      241 ttgtggtgcc ttcctgggct tgcccagcaa attgctgtgc cggaactgcg ccaacaggtg
      301 accgatatta ctggtacgtt aagcacttct gaacaacaat cactgacaca gcaactgcag
      361 gatattacac ataaaacacg ggcgcaggtc gctgtattag tagtgccgtc caccggagac
      421 gattccattg aacaatatgc tacaagagtt tttgacaatt ggcgtttagg agatgccaaa
      481 cgtaatgatg ggatactgat cattgttgcc tggtcggatc gtactgtccg catccaggta
      541 ggttatgggc tggaagaaaa ggtaaccgat gctctggctg gcaatatcat ccgtagcatc
      601 atgatacccg cctttaaaaa acaaaaatta gctaagggat tagagttagc tataatcgct
      661 ttgaataacc aactcacttc acaacaccaa tattcgtcta atccttcaga aagtgaatca
      721 gcgtcttcca gtgatcatta ctactttgct attttttggg tatttgcagt gatgttcttc
      781 cctttctggt tttttcatca aggcaataat ttttgtcggg catttaaaag cagtgtttgt
      841 atttcaacag tctatcttat agatttattc ctgttctccg atcaaacttt ttccattgct
      901 gtattttcct tcttttttgc tttcaccatc attatggtct ttacctggtt atgtgtccca
      961 cagaaaaaag catctggtag aagttatcat tcagacaaca gcggttccgg tggtggctca
     1021 agtggcggcg gatttagcgg cgggggcggt tcttctggcg gtggcggcgc gtccggtcgc
     1081 tggtaactca accataaaaa atgccagccc gaaggctggc atttttaaat cagataaagt
     1141 cagacttatg cctggccttt gatctcttta cgaccgttgt acggtgcttt ttcgcccaga
     1201 gcttcttcga tacgaatcag ctggttgtat ttagcaacac ggtcagaacg gctcatagaa
     1261 cccgttttga tctggcctgc agcagtacca acagccaggt cagcgatggt agcgtcttca
     1321 gtttcgccag aacggtgaga gataactgca gtgtagccag cgtctttcgc catcttgatt
     1381 gcagccagag tttcagtcag agaaccgatc tggttgaatt tgatcaggat ggagttagcg
     1441 ataccttttt cgataccttc tttcaggatc ttggtgttgg ttacgaacag gtcgtcacca
     1501 accagctgga ttttgtcgcc cagaactttg gtctggtatg cgaaaccatc ccagtcagat
     1561 tcgtccagac cgtcttcgat ggaaacgatc ggatactgtt tggtcagttc ttccaggaag
     1621 tgagtgaatt cttcagaggt gaatgctttg ttgccttcgc cagccagaac gtatttaccg
     1681 tctttgtaga attcagaagc tgcgcagtcc atcgccagag tgatgtcttt gcccagttca
     1741 taaccagcag ctttaacagc ttcagcgata acagccagag cttcagcgtt ggaacccagg
     1801 ttcggcgcat agccaccttc gtcaccaaca gcagtgttca tgcctttcgc tttcagaact
     1861 tttgccaggt gatggaaaac ttcagaaccc atgcggatgg cttctttcac agttttcgcg
     1921 ccaaccggct gaatcatgaa ttcctggata tcaacgttgt tgtcagcgtg ctcaccaccg
     1981 ttgatgatgt tcatcatcgg aaccggcata gagtatttgc ccggagtgcc gttcagttca
     2041 gcgatgtgct cgtacagcgg cataccttta gcagctgcag cagctttggc gttagccaga
     2101 gatacagcca ggattgcgtt cgcgccgaat ttggatttgt tttcggtgcc gtccaggtcg
     2161 atcatgatct tgtcaatgcc agcctgatct ttagcatctt tgccaatcag cgcctgagcg
     2221 atcgggccgt ttaccgcagc aacagctttg gttacgcctt tacccaggaa acgggatttg
     2281 tcgccatcgc gcagttccag agcttcacgg gaaccagtag aagcacctga cggagcagct
     2341 gccataccga cgaaaccacc ctccagatgt acttcggctt caacagtcgg gttaccacgg
     2401 gagtcgatga tttcacgacc gatgattttt acgattttgg acattaggtt ttcctcaagt
     2461 cactagttaa actgaaactc cagacaaaca acgcgtaccc agggtacgcg ttgccgttct
     2521 aactttttta cttacttcgc ctgacgtttc tggaactcgc tggcggcttt cacaaagcct
     2581 gcaaacagcg ggtgaccatc acgtggagta gaagtaaact ccggatggaa ctggcaagcc
     2641 acgaaccacg gatgattcgg aacttcgatg atctcgacca actgatcatc cccggaacgg
     2701 cccgcaacgc gcagacctgc atcttcaatc tgtttcaaca gcatgttgtt gacttcgtaa
     2761 cggtgacgat gacgctcaac aattgtcggc gcattgtaca gctggcgaac caggctatcg
     2821 tcaaccaact ggcactgctg tgcgccgaga cgcatggtac cgccgagatc gctcttctcg
     2881 ctacgaactt caacgttgcc gttttcatcg cgccactcgg taatcagcgc cacaaccggg
     2941 tacttacagt ctggcacaaa ttccgtagag ttggcgttct ccatgttggc aacatggcga
     3001 gcgtaatcaa ttaacgctac ctgcataccc aggcaaatgc ccagataagg aatattgttc
     3061 tcacgcgcaa aacgcgcggt cgtaatcatg ccttctacgc cacgatagcc gaaaccgcca
     3121 ggtacgagga ttgcgtccag acctttgagg atttcaacgc cgcgcgtttc aacatcttgt
     3181 gaatcgatca gtttgatgtt gacgctgaca cgattcttca gcccaccgtg tttcagtgct
     3241 tcgatcacgg atttgtatgc atccggcagc tcaatgtatt tgccgaccat accaatggtg
     3301 acttcactta ccggattcgc ttcttcgaag ataacctgtt cccattcgga cagattcgct
     3361 tccgggcagt ttaagctgaa tcgtttacaa atataatcgt ccagcccctg agatttcaac
     3421 aggcccggaa ttttatagat ggaatcgacg tctttcagag aaataaccgc tttttccgga
     3481 acattacaga acaatgcaat cttcgcacgt tcgttcgccg gaacggcgcg atctgaacga
     3541 caaatcagga tatcaggctg gataccgatt gaaagcagct cttttacaga gtgctgagtc
     3601 ggtttggttt tgacttcacc agacgctgcc atgtacggca ccagcgtcag gtgcataaac
     3661 agggtgtgct cacggccaat ttcaacagcc atctggcgaa tcgcttcgag gaacggcagg
     3721 gattcgatat cacctacagt accgccgatt tcgaccagta ctacgtcatg accttcgccg
     3781 ccttccagca cgcgctcttt gatagcgtta gtgatgtgcg gaataacctg tacggttgcg
     3841 ccgagatagt caccgcggcg ttctttacgc agaacgtcag agtagatacg ccccgtggtg
     3901 aagttgttgc ggcggctcat tttggtacga atgaaacgct cgtagtgccc caggtccagg
     3961 tcggtttcag cgccgtcttc agtaacgaac acttccccgt gttggattgg gctcatagta
     4021 cctggatcga cgttgatgta cggatccagt ttcatgatgg tcacattgag gccacgggct
     4081 tcaagaatgg ctgcgaggga ggctgcggca atgcctttac ccagagagga tacgaccccg
     4141 ccggtcacaa aaatatagtt cgttgtcatg ctgaacctga gaagttaggt tgaaaagacg
     4201 atggaataac caggacggga aagcagtata cccgaacatg acctgtgcca caaactttca
     4261 ttatccctcc tcttcgccag cgcactattg aaatcaggag tgagaaaata gcccctttgg
     4321 ggtaaatgtt tttgacgcaa atcaagcgct tgtcatttaa aaaatgacac aaatcgcgct
     4381 tgaccgcgta attcccttag agatcaattt cctgccgttt tacctgttgc cagacttctt
     4441 ccattgtttc gaggtcaaca cctgtcattt ccagtccacg cgcggcaaca atacgctcca
     4501 cttcgcgaaa acgacgctcg aatttttcgt tcgctttttg caatgcgatt tccgcttttg
     4561 tccctaaatg acgagccaga ttgaccgtgg caaacagcag gtcacccatt tcctcctcca
     4621 gtttagcctg gtcgacaaca gcctgccgcg cctcgtacat cacctcgtcg atctcttcgt
     4681 agactttatc gactaccgga ccaagcgtcg tccagtcgaa accaacgttg gcgcaacgtt
     4741 tctggatttt ttgcgcacgc attaaagccg gtaaactacg aggaatatcg tccagcgccg
     4801 aatgctgcgc tttctgcgcg cgctcttcgg ttttgatttg ctcccaacgg gcaagcactt
     4861 cactactatt ttcggcagaa ctgtcagcaa aaacatgcgg atggcgacgc tctaatttat
     4921 cgctaatagc agcgcaaata tcattaaagt caaagcgccc ttcttcctga gccatttgtg
     4981 cgtaaaacac cacctggaac agcagatcgc ccagttcacc gcgcagatcg tcaaaatctt
     5041 cacgggcgat ggcgtccagc acctcgtagg tttcttcaag ggtgtaaggc gcaatggtgg
     5101 caaatgtctg ctctttatcc cacgggcagc cgttttccgg gtcgcgcagg cgctgcatga
     5161 tggtgagcaa acggtcgatt tgattcattg aattatcctg aaaattgcgg gtctgtcagg
     5221 tggaaacctg tgaccagaat agaagtgagt tagtaacacc tacccaatca gcacgttaat
     5281 tttggcttta atgagttgta attcctctgg ggcaactgtt cctttcttcg ttgctcctct
     5341 tgcccgccag gcgatacttt ttacctgatc agctaacgct acgccatcac gttcctgacc
     5401 ggatagaaca acttcgaacg gatatccttt tgattgcgtt gtacaaggaa cacacagaca
     5461 catacctgtt ttgttgttgt acatgaacgg actcaggaca acagccggac gatgtccggc
     5521 ttgctcgcta ccttttgtcg ggtcaaaatc aacccaaatc agatcgcccg tatcgggtac
     5581 gtatcggctt accattacca gacttcctta tctttcggct ccccccagtc gatattctcg
     5641 tggaggtttt ccggcgtgat gtcgttgacc agttcagcaa gcgtaaatac gggctcttta
     5701 cgcactggct caataattaa tttgccatcc accaggtcaa tcttcacttc atcatcaata
     5761 ttcagattga gcgcctgcat taacgtagcc gggatccgca ccgccggtga atttccccaa
     5821 cgctttacgc tactgtggat cataaccctt tcctcaaacc gcgatcatat gtagatacag
     5881 tatatatcaa tctacattgt agatacgagc aagtttcggc ctaactcccg tgcaaccgac
     5941 gcgcgtcgat aacatccggt acctggttga gtttacccag cacgcggccc agcacttgca
     6001 ggttgtaaat ctcaatggtc atgtcgatgg tcgccagttg ctgtttggtg tcgctacggc
     6061 tggcaacgcc aagcacgttc accttctcgt tggcgagaat ggtcgtgata tcacgtaaca
     6121 acccactacg atcattagcc actacgcgga ccaccagcga atatccggcg gagtagctct
     6181 caccccatac cgcgtcaaca atgcgttctg gcgcatggga gcgcagttcc gccagttgtt
     6241 cgcaatcggc gcggtgtact gaaataccgc gtccctgagt aatgaaacca actatttcat
     6301 caccaggaat aggctgacag cagcgcgcga tgtggtgcat caggttaccg acaccttcaa
     6361 ccaccacacg accgttatct ttactgcggt tttgcggcgt gtagcttttt tgctgaagct
     6421 gtttcagcgc ggcggcgtcc tgctcttcgg cactcggctt attaaattgc gattgcagga
     6481 agttcaccat ctgattgaga cggatatccc cgccaccaat cgccgccagc aactcgtcga
     6541 catcattgaa gttgtaacgc ggcagcagat gtttttctgc ttctttcagg ctgatcccca
     6601 gatgttccag ctcgtcgtca aggatttgcc gcccagccag aatgtttttg tcacggtcct
     6661 gtttacggaa ccaggcgtga attttcgaac gcccacggct ggttgtgacg taaccgaggt
     6721 ttgggtttaa ccagtcacgg ctggggttcg gctgtttctg ggtgataatt tcaatctggt
     6781 cgcccatctg cagctggtag gtgaacggca caatgcgccc gccaattttt gccccgatgc
     6841 agcggtgtcc gacatcactg tggatgtggt aagcgaagtc cagcggcgtt gatcccgcag
     6901 gcaaatcaac gacatcacct ttcggcgtaa agacgtacac tcggtcgtca aagacctgac
     6961 tacgtacttc gtcgagcatt tcgccggaat cagccatctc ttcctgccac gcaatcagtt
     7021 tacgcagcca ggcaatccgg tcttcatgtc ccgaacgtgc gccgccagca gccgcgccct
     7081 ctttatattt ccagtgcgca gcaacaccca actctgcatc ttcatgcatc tgtttggtgc
     7141 ggatttggat ctcaacggtt ttgccacccg gccccagaac cacggtatga atggactgat
     7201 aaccgtttgg tttcgggtta gcgacgtaat cgtcaaactc atccggcagg tggcgatagt
     7261 gagtgtgcac tatccccagt gcggcataac aatcctgtaa acgctcggcg acaatacgta
     7321 ccgcacgcac atcaaacagc tcatcgaagg cgaggttctt tttctgcatt ttgcgccaga
     7381 tactgtagat gtgtttcgga cggccataca cttcagcttt aacgccttca gctttcatct
     7441 cagcgcgcag atggccgaca aactcttcga tatagtgttc gcggtcgaga cggcgttcat
     7501 gcagcagttt tgcgatgcgt ttgtattcgg tcgggtggag ataacggaag cagtaatctt
     7561 ccagctccca tttcagttgc ccaatcccaa gacggttcgc caacggcgcg tagatattgg
     7621 tgcactcttt tgccgccagt acgcgttcat cttccggcgc atcttttact tcgcgcaggt
     7681 gagcaatacg ctccgccagt ttgatgacca cgcagcgaaa atcatcgacc atcgccaata
     7741 acatccggcg aacgttatcg acctgttcgg aggaaacaga atcagtgtgc gtcgctttca
     7801 gctggcggat cgccgccata tcacgcacgc cgtgaataag gttaacgacc gacttaccga
     7861 cgctctcacg cagcacatct tcgctgacta cgttggcatc agccagaggg aacagcagcg
     7921 ccgcccgcag cgtgtcaatg tccatactta atgtcgagag gatctccacc atctcaacac
     7981 cacgccacaa taacagactg gcatccggat gcccctgcgt ctgttgcaga caatacgccc
     8041 aggtttcggc taagcactca cacgacttct ggctggtaat acccagactt gcgatccatt
     8101 tttccggatc aaattcacca gccttattga tatgtgcact tcttaccgca accatcgtcc
     8161 tctcctttag ggaccagacc tgccgaaatc ggcaaatcgt aactatttaa cgcgcgagaa
     8221 aagtaccatc gattccagat gtcccgtgtg tgggaacata tccagcatcg ccagtcgcgc
     8281 aatggtatat cctgctttta ataacgcttc gctatcccga gccagcgttg cagggttaca
     8341 ggatacataa actatacgaa taggttccag ttttataatt tgctgcataa cacctgcggc
     8401 acctgctcgc gccgggtcca acaacacttt atcgaagccg tttttcgccc acggctgctt
     8461 tgtgacatct tcttcaagat tttcgtgata aaacgtcaca ttctgtaagc cattaagacg
     8521 cgcattctgc tggccttttt ccaccagcgc cggaacacct tctacaccca ccacactggc
     8581 agcttgtgtc gccaatggca gtgtaaagtt gcccataccg cagaacagat ccagtacgcg
     8641 atcttcaggt tccacatcca gccattccaa cgcacgcgct accattttct ggttcacacc
     8701 cgcattgacc tgaataaaat cgcgcgggct aaaagttaag cgcaacccat ttgagtcata
     8761 ccagggcatc tcaccagaga cggtttcgag tatctcacta tcgggggcga gatacagatc
     8821 caggccttca gaatgcgaaa agcattccag tttttcgcga tctgccgaac ttagcggtgc
     8881 ggtatggcgc aaaatcatta gcgtgccgct ggttgcctgt accagttcaa catgaccaag
     8941 atggcgcata gcttgtaagc tgcccaggca tgccctgact ttgggcagca atgcttcaag
     9001 ttggggcact aaaatggggc attgtttcac gtcgacaatg tcactggagc ccgctttgcg
     9061 aaaccccatc tgaagttgct gtgttttcgg taagtagttc agacttaaac gcgcgcgacg
     9121 gcgatagccc cagggaacat cggcgatcac ttcagagact tcgtgtttca ttaatcgggc
     9181 gagtgccgca cttttgcttc gctgctgtaa atccacactg gcgtgttgtt gctgacagcc
     9241 gccgcatacg ccaaaatgag gacagcgtgg cgtttcgcgt tccgggctat cgcttaaccg
     9301 acgtacgact ttagcgcggg catactgttt tttatcttca gtaacagtaa cttccgcgtt
     9361 ttcctgcggc aataatccgg ggataaacaa cgttttgccg ttatgtcgcg ccacgccctg
     9421 accaaaagag tcgaggtcgt tgactgaaac ggttatgatc tgacgcgtcg tcgtgcgtcg
     9481 ttttgcagag tagaattgcg ccattggcga gactttctca atttaacagt gtgaccttaa
     9541 ttgtcccata acggaactcc atgaccaact acagcctgcg cgcacgcatg atgattctga
     9601 tcctggcacc gaccgtcctt attggtttat tgctgagtat ctttttcgtc gtgcatcgct
     9661 ataacgactt gcagcgtcaa ctggaagatg ccggtgccag tattattgag ccgcttgcag
     9721 tttctactga atatggcatg agcctgcaaa atcgcgaatc tatcggtcag ttaataagcg
     9781 tactgcatcg tcgccattcc gatattgttc gcgcgatttc ggtttatgat gaaaataacc
     9841 gactctttgt cacctccaat tttcatcttg atccctcatc aatgcagctc ggcagcaacg
     9901 tgccgtttcc tcgccagctc actgtcactc gtgacggcga tattatgatc ctccgcacgc
     9961 cgattatttc tgaaagttac tcccccgacg aatcgcccag tagcgatgcc aaaaatagtc
    10021 aaaatatgct gggatatatt gcgctggagc tggatcttaa atcggttcgc ttgcagcaat
    10081 ataaagagat ctttatttcc agcgtgatga tgctgttttg tatcggtatt gcgcttattt
    10141 ttggctggcg cttaatgcgc gatgtaaccg gtccgattcg caacatggtg aataccgtcg
    10201 accgcatccg tcgcgggcaa ctcgacagcc gggtggaagg atttatgctc ggcgagctgg
    10261 atatgctgaa aaacggtatc aactcgatgg caatgtcgct ggcggcttat cacgaagaga
    10321 tgcaacacaa tattgaccag gcgacgtccg atctgcgtga gacgctggag caaatggaga
    10381 ttcagaacgt tgagttggat ctggcgaaaa agcgcgccca ggaagcggcg cgtattaaat
    10441 ccgagtttct ggcaaatatg tcacacgagc tgcgtacacc actgaatggt gttattggct
    10501 ttacccgcct gacgctgaaa acagaattaa caccaacgca gcgcgatcac ctgaatacga
    10561 ttgaacgttc ggcaaataat ttgctggcaa ttattaatga tgttctcgac ttctcgaaac
    10621 tggaagccgg taagctgatt ctggaaagta ttccattccc actacgcagc acgctggatg
    10681 aagtcgttac cctgctggca cattcttctc acgataaagg gctggaactg acgctcaata
    10741 ttaaaagcga cgtgcctgat aacgtgatcg gcgacccact acgattacag caaatcatca
    10801 ctaacctggt agggaatgca attaaattca ccgagaatgg caacatcgat attctggtag
    10861 aaaaacgtgc gctgagtaat accaaagtgc agattgaagt gcagattcgg gataccggca
    10921 ttggtattcc tgaacgcgat caatcgcgct tattccaggc cttcagacag gctgatgcca
    10981 gtatttcccg ccgtcatggt ggcaccgggc tggggctggt gattacacaa aaactggtta
    11041 atgaaatggg cggcgatatt tcgttccata gcctgccgaa tcgtggttca actttctggt
    11101 tccacattaa tctcgatctg aacccgaaca ttattatcga agggccatcc acccagtgcc
    11161 tcgctggtaa gcgcctggcc tatgtcgaac caaactccgc agcagcacaa tgcacgctgg
    11221 atattttaag tgaaacgccg ctggaagtgg tttatagccc aacgttctcc gcgctgcctc
    11281 ccgcgcatta cgacatgatg ttattaggca tagcggtgac cttccgcgag ccgctaacaa
    11341 tgcaacatga gcgattagcg aaagcagtat cgatgaccga tttcctgatg ctggcacttc
    11401 cttgccatgc acaagtcaat gctgaaaaac ttaagcaaga tggtatcggc gcgtgtctgc
    11461 tgaaaccatt aacacctacg cgcctgttgc ctgccctgac ggaattttgt catcacaaac
    11521 aaaacacgct tttgcctgta accgatgaaa gtaagctggc aatgacagtc atggcggttg
    11581 atgacaaccc cgctaacctg aaacttatcg gcgcattgct ggaagatatg gtgcaacatg
    11641 tggaactttg cgatagcggg catcaggcgg ttgaacgggc gaaacagatg ccgttcgatt
    11701 taatcttaat ggatattcaa atgccggaca tggacggcat tcgggcatgt gagctcatcc
    11761 accagctccc acatcagcag caaacgccgg ttatcgcggt aacggcgcat gcgatggccg
    11821 ggcaaaaaga gaaactgctt ggcgcgggga tgagtgatta tctggccaaa ccgatcgaag
    11881 aagagcgatt gcataatttg ctgttgcgct acaagcctgg cagcggtatt tcctctcgcg
    11941 tcgtgacgcc cgaagtcaac gaaattgtgg tcaacccgaa tgcgaccctc gactggcaac
    12001 tggcactacg ccaggcagca ggaaaaaccg atttagcgcg cgatatgctg caaatgttac
    12061 tcgatttcct gcctgaagtt cgcaacaaag ttgaggaaca gctggttgga gaaaacccgg
    12121 aaggcctggt tgatttgatt cataaactgc atggcagttg cggctatagc ggtgtaccgc
    12181 gtatgaagaa tctctgccaa ctgatcgaac aacagctacg tagcggtact aaagaagaag
    12241 atttagagcc ggagctgctg gaactgttgg acgagatgga taatgtagcg cgcgaagcca
    12301 gcaaaattct cgggtaatgg acgtcttgtc gagtagcaac cggattttta tgacgctggt
    12361 ttcagattgg tgacaagtgc ctgtgtttat gccggatgcg gcgttaacgc cttatccggc
    12421 ctgcaaaacc aattaaattc aatgaattaa aaaatgatgt agccctgata cgtagcgcaa
    12481 caggcaattt tgcgtttggc atcagtctca aaccggctcc agatagagcc ggttttggtt
    12541 ttctgtctta acgcaccatg cacggacgct tgttatcgaa cgtccagccg gggatcaggt
    12601 actgcattcc catcgcatcg tcacgcgcgc caagcccgtg tttctgatac agctcgtgcg
    12661 ctttcatcac ttgatccata tcgatttcta cacccagccc cggtttttct ggcacctgaa
    12721 ccagaccgcc tttgatctct aacggttctt tggtcaggcg ctgattacct tcctgccaga
    12781 tccagtgcgt atcaatcgcg gtgattttac ccggtgcggc agcggcaaca tgggtaaaca
    12841 tcgccagaga aatatcgaag tggttgttgg agtgtgaacc ccaggtcagg ccaaattcat
    12901 gacacatttg cgccacacgt accgaacctt gcatcgtcca gaaatgcgga tccgcaagcg
    12961 ggatatcaac ggattgcaac gagagcgtat ggcccatctg ccgccagtcg gtggcgatca
    13021 tattggttgc ggtcggcagg ccagttgcgc gacggaactc tgccatcact tcacgcccgg
    13081 agaaaccttg ctccgcacca cacggatctt ctgcataagc cagcgaacct ttcaggtatg
    13141 taccgatttt aatcgcttcg ttcagcgacc aggcaccgtt aggatcgagc gtaatacgcg
    13201 cctgcgggaa acgttgcgcc agtgccacaa tagattcggc ttcttcttcc ccggccagta
    13261 caccgccttt cagtttgaaa tcgttgaagc catatttttc atacgccgct tccgccaggc
    13321 gcaccaccgc atccggcgtc atcgcttctt catgacgcag gcgataccag tcgcatgagt
    13381 catccggctg gctttgatac ggcagcggtg tagctttgcg attaccgacg aagaacagat
    13441 aaccgagcat ttcgacttcg ctacgctgtt gaccatcgcc cagcagcgat gccacgttta
    13501 cccccagatg ctgccccagc agatccagca ttgccgcttc tatcccggta acaacatgaa
    13561 tagtggtacg caaatcaaat gtctgcaaac cgcgcccacc ggcatcacga tcggcaaaag
    13621 tattacgcac cagcgtcaga acgtttttgt attcacccag cgttttaccc accaccagcg
    13681 gaatcgcatc ttccagcgtt ttacggattt tctcgccgcc gggaatttcc cctacgccag
    13741 tgtgaccgga attatctttg ataatcacaa tattacgcgt gaagaacggt gcatgtgcac
    13801 cactcagatt catcagcata ctgtcatgac ccgccaccgg gatgacctgc atttcagtaa
    13861 caacaggcgt cgtaaattga gaactcatat ttatgtcctt tttcagaatc aatgacggcc
    13921 gaaaacggga cgtttacggt caaaggtcca gccggggatc aggtactgca tcgggcctgc
    13981 gtcgttacgc gcaccgccag gcagacgttt ataggcctca tgtgcctttt gtacctgttc
    14041 ccagtccagt tccacgccca gaccgggcgc atcaggaacg gcaatttttc cgtttttaat
    14101 ctccagcgga tttttggtca gacgacaatc gccctcctgc caaatccagt gggtatcgat
    14161 agcggtagga ttacccggtg ccgccgcgcc cacatgggta aacatcgcca gagagatatc
    14221 aaagtggtta ttagaatggc agccccaggt cagcccccag tcgtcgcaaa gttgcgccac
    14281 gcggactgca ccggaaagcg tccagaagtg cggatcggca agtggaatat ctaccgcatt
    14341 gagcatcacc gcatgaccca tttcgcgcca gttggtggcg atcatgttgg tcgcgacggg
    14401 caagccggtc gcccgtcgaa actccgccat cacttcacga ccggagaaac cctgctcagc
    14461 gccgcacggg tcttccgcat aggtaagaac atcatttagc cctttgcaca gagaaatggc
    14521 ttcatcaagc agccaggcac cattgggatc aacggtaatc cgcgcatcag ggaagcgttt
    14581 cttcaatgca cgaacagtgt cgatttcttg ctcgccaggt aacacgccac ccttaagttt
    14641 gaaatcttta aagccatagc gatcctgtga ggcttccgcc agacgcacaa cggcttcgct
    14701 gttcatcgct ttctgatgcc gcaactgata ccactcatgg ttgcccggcg tattttccag
    14761 ataaggaaga tcggttttgg tccgatcacc gatataaaac agataaccga gaacggtaat
    14821 agcatcacgt tgcttgcctg gccctaacag ttcgcagacc ggaacattca gcgcctgacc
    14881 taacaggtca agcaaggcgg cttccagcgc cgccacggca ttaacgcgca attcaaaagt
    14941 ccaggcacct ttgccgaagg tatcaaaatc ggctgcctga ttacctttat gcacctgctg
    15001 aaccacttta ttcaggcgcg caacttcctg gcccagcacc atcggaatag cctcgaccag
    15061 tgtttgataa atcacctctc cgcccggcgc ttcaccaatg ccggtatgcc cggcattatc
    15121 ggtgagtacc acaatattgc gggtgaaata tgcgttatgt gcaccaccaa tattaagcaa
    15181 catgctgtca tgtccggcga ccggaatgac tttcatatca gtaataacag gactggattg
    15241 tgtcgtcatc ttattgcccc gcgacaggtt tcagctcaat acgcttgata tcgcccacca
    15301 gcaccaggta gctgagtacc gcgattaagg catgaacacc aacataaatc agcgccccat
    15361 taaaggagcc agtcgtgcca acgatataac caattgcgat tggcgtgacg atgccagaaa
    15421 tgttaccaaa catgttaaac aggccaccgg aaagaccgct gatctctttt ggcgcagtat
    15481 cagccattac agcccaaccc agcgcaccga tccccttacc gaagaaggcc agcgccataa
    15541 agccgatgat catccactca acgttgacgt agttgcagaa caccatcacc atcgacaaca
    15601 acatgcccat cacgatcggt gttttacgcg caatgttcag tgaacccgtg cgacgcatca
    15661 gccagtcgga aataatccca cccagcacac cgccgataaa accgcaaacc gccggaacgg
    15721 aagccacaaa gcccgctttc agaatcgaca tcccgcgcgc ctgcaccaga taaaccggga
    15781 accaggtaat aaaaaagtaa gtcagggcgt tgatacagta ctgaccgata taaacgccga
    15841 tcatcatccg tgacccgagc agctgtttga tctgccccca cttcacgcta aacggcactt
    15901 taactttggt gttttgctga tccatattga tcagcgcgcc acccgcggcg atgtactcca
    15961 gctctttctg atttacgccc ggatgttgat ttggctcgtg gatgactttc aaccagataa
    16021 agctgatgac aatgcccaga ccgcccataa agaaaaagac gtgtgaccag cccacttcat
    16081 gcgtcagcca gcccataatc ggcgcgaaga taaccgttgc gaagtattga gcggagttaa
    16141 aaatcgacac cgccgttccc ctttcctgcg ccggaaacca ggccgcaaca atgcgactgt
    16201 tgccggggaa agagggcgct tcagcaagcc cgaccaggaa gcgcagcgta aacagggcaa
    16261 cgataatgcc gaatccacta aagatatcga cgaagccttg cagcaaggta aacatcgacc
    16321 agataaagat cgaccagaag tagacgcgtt ttgaaccaaa acgatccagc aaccagccac
    16381 cagggatctg cccgataaca tacgcccatg agaaggcaga gaacacatag cccattccca
    16441 cgggatcaag gccgatatct ttggccattt ccgaaccggc aatagaaagc gtagcgcggt
    16501 cgccgtagtt gaaggatgtg acgataaaca acatcaccac tatccagtaa cgagcattcg
    16561 tgcgtttttc cacactgctc gcagcctgac ttaaagaact cattgttgca ctcctgaaaa
    16621 ttcgcgttag ccacgctcac tctggactgc gacatcgcca ggaaatcaga ggtgacgtag
    16681 ggtgtttttg ccgtttttat aggtcgttcg ccgaatacgg cgcgtgttta tatctggcaa
    16741 tagcagtata aaaagcgcgc catagcggct caccgtgcaa caacacaaca ttaatgcgtt
    16801 caatgaggcc cgattttggc attagccctg gacggtggaa tccacttcac ggaaatgaaa
    16861 acaagaacaa gaaaggaagg gttaaaacga agaaataaag aagagtatga aatggatcgc
    16921 ttgactccag gcaaacgcca gtaaaaatcc gcgctatgaa gcagttttta ctggcatttg
    16981 cctgaaaaga ttcgattcag caccgctaaa acggcattta ccgctcgctg aacatatcag
    17041 gacaacatcg tgccccaatg ttcaacccac ggatttgatt ccgtttccgg ttccgggttc
    17101 tcactggcgt caatcaacag catttcgcca acccgctgag cgctctgttc ctgcaacaag
    17161 gcatcgaact gtttgccgcc attgcagaaa ttcacataac tactgtcgcc gagcgcaatc
    17221 acgccataac gcagattcgg ctggaagccc agactatctt tgattccctg aaagagtggc
    17281 acaatgctat caggaaggtc gccctgcccg gtcgtggacg taaccaccag aacatactta
    17341 tcctgatagg gcagccagtc gcttaattca ggatcttcaa ataccgttgc tttgtggccc
    17401 tgcgcggtca gaatcgcttc cgcttcttcg gccactaaca gtgaattccc gtacatggtg
    17461 ccgacaaaaa taccaatttc cgccataccg ttttctccct ggattaggaa cttatctctc
    17521 catcctgacc cgatggcgca ctgaactcaa ccctttcatt ttcaggaagc agaccgcgcc
    17581 agccaaattg tgataacgcc tgcatccagg tgtcgtccag gcccgcgtga atagtcagcg
    17641 gctcgccagt aaaaggatgc gtcaatgaca actgactggc atgcagcatt aaccgctgaa
    17701 ggccaaaatg ttcagcaccg ctgcgattct ggcgtaaatc accatgtttg ctgtcgccaa
    17761 taatcgggtg gcgcaaatgg gcaagatggc gacggagctg gtgcttgcgt ccggttttcg
    17821 gctccagttc caccaggccg tagcgcgtgg tcgggtaacg tccggtcgct accagcattt
    17881 ctacggtcgc cagaccgcga taatgcgtca ctgctggctg cgggccttta tcttcgcggg
    17941 caaatttatc agcgattttg tccagttctt ccaccagtgg ataatccagc accgcttctt
    18001 ccatcaacca gccacgcaca atcgcatggt aacgtttctg gatttggtgc tgttcaaact
    18061 gttgtgccag cagccgtccg gcttcgctgg ataatcccat caacaacaca ccagaagttg
    18121 gtcggtccag acgatgagca gtaaaaacat gctggcctat ctggtcacgc acggtttgca
    18181 tgaccactac tttctcgtcg cgatccagcc agctgcggtg aaccagccag ccggagggtt
    18241 tatttaccgc aaccagccat tcatcctgat agagtatttc cagcattagc tcgcatcatc
    18301 cgcaaaaaga gcatccagtt tctccagctc agccagaata agcgcgcgtt gcggatgatc
    18361 agtcaccagc gccatttcat aatagggcgc aacagcaaaa gcgcccggta acggctggtt
    18421 gttatctaac aaatcgtgca ttcgcgggat cagcacccac tgcaaccact ccagtggttc
    18481 catggtgtcc ataaagaacg gttgagtact gttaaattga tgcggcaggg gttcatcgtt
    18541 tcgccagtgc tgatgttcac gcagtaacgc ttcaagcgcc tgcaactgga gacgaacgcg
    18601 gtcatgagtg gtcataaagc aacctcaata agaaaaactg ccgcgaagaa tagcaggaat
    18661 aaaaaaaggg agcactgtat tcacagtgct cccggttcgt ttcgcagcat tccggctact
    18721 tttgttgctc cctgctcatc cttgacaact tttcctctgg ccttgcggcc aatcgttcat
    18781 cctgaactat tgcttcctgc tcacaccacc ccgatgtgat acttcatcct gaagtgttcc
    18841 tggccttcct gacccaccga atcatcctga ccggttctca ttctccatcc tggaggtgtc
    18901 ctttaacgcg tcctgcgtca tcctcttcgc ttcatccaga agcctttccc tgaaacacca
    18961 tcctggtgtg tccttcagaa gtgtcatcat cctgatgttc acttcgttgt ctgactccct
    19021 gtcgacgaag atagaatcgt ctttttctcg aagtcttaca aggcgcttac agacaatgcc
    19081 ttaaggaaaa tttcatatga aagttaagaa ttaacaagca acatgttgtt tttaaatgag
    19141 aataacgatt cttatctttt taagtattcg cttatttcct ggcgatctcc tacagagtct
    19201 gtaagagatc tctcacaacc agatggggta tttggattac agaagaggct taagttgatt
    19261 aaggaattcc gcaagatttg aggctaaatg cgtccgttta cgtgttccca gcgtctcttt
    19321 gcacacttca ccggagagat tacacaccga gatgacctct aactcttctt ccagcgtcgc
    19381 gataaatagc gtgggcggca gttttagcct tttctgagtc accagatgac caatcagatt
    19441 ttcctgcacc cgacggaagt cgtcttcact ccaggtttgc agcaatgtca gtttgatatc
    19501 gccaaactgc gcgtgcatat ccccggcaaa ctgagtcgta taaaaggtat gaatcgtggg
    19561 ttgtatcaca atatcaaaag cgcgttcaac cgcgtttaca ttttgttccc cggtaaacgg
    19621 ctggggttgc cagtatacgg catcttcggt agttgaaata atgcacggtg aaggaacgcc
    19681 gtacagttcc tcacttaacg gccagctttt atgctcttca tgccacgcat cgcagtagcg
    19741 tgcagtaaaa tctttcaggg cttgtgcggt caaatcgtcc actaatttct ctcttcactt
    19801 aaaccagata cacttgtcgt ttagtttatc tggtttatga cggtgaaaca tgtcttctta
    19861 tgcaaaccat caggcacttg cgggcctgac acttggaaaa tcaaccgatt accgggatac
    19921 ctatgacgcc agcctactgc aaggcgtacc acgcagcctg aatcgcgacc cgctgggtct
    19981 gaaagcggat aacctgcctt ttcacggtac ggatatctgg acgctgtatg aactttcctg
    20041 gctgaatgcg aaaggtttgc cgcaggtcgc tgtcggtcat gttgaacttg attacaccag
    20101 cgtaaatctg attgagtcga agagttttaa gctctatctc aacagtttta accagacgcg
    20161 ttttaataac tgggatgagg tgtgccagac gctggagcgc gacttaagca cttgcgccca
    20221 gggtaaggtt agcgtggcgt tatatcgtct tgatgaactg gaaggccagc cgataggtca
    20281 tttcaatggc acctgcattg atgaccagga tatcactatc gataactatg aattcactac
    20341 tgactatctg gagaatgcca ccagcggtga aaaagtagtg gaagagacgc ttgtcagcca
    20401 cctgctgaaa tcaaactgcc tgatcaccca tcaaccagat tggggttcga tccaaattca
    20461 gtatcgtgga cgccaaattg acagagaaaa actgctgcgt tacctggtct ctttccgtca
    20521 tcacaacgag ttccacgaac agtgcgtgga acgcatcttt aatgacctgt tacgcttctg
    20581 ccagccagaa aaattgagcg tttacgcacg ttatacccgt cgtggcggtc tggacattaa
    20641 cccgtggcgc agtaataacg attttgtccc atcgaccaca agactggttc ggcaataaat
    20701 tttttctcaa ttttgcgtgc tggattcacg cagaaggttg tgaaaggtca gcaggcaggg
    20761 ctattgtaat caaagggaat gacgatattc gtcccataag gagtttttct tgattacaca
    20821 tattagcccg cttggctcca tggatatgtt gtcgcagctg gaagtggata tgcttaaacg
    20881 caccgccagc agcgacctct atcaactgtt tcgcaactgt tcacttgccg tactgaactc
    20941 cggtagtttg accgataaca gcaaagaatt gctgtctcgt tttgaaaatt tcgatattaa
    21001 cgtcctgcgc cgtgaacgcg gcgtaaagct ggaactgata aatcccccgg aagaggcttt
    21061 tgtcgatggg cgaattattc gcgctttgca ggccaacttg ttcgcggttc tgcgtgacat
    21121 tctcttcgtt tatgggcaaa ttcataacac cgttcgtttt cccaacctga atctcgacaa
    21181 ctccgtccac atcactaacc tggtcttttc catcttgcgt aacgctcgcg cgctgcatgt
    21241 cggtgaagcg ccaaatatgg tggtctgctg gggcggtcac tcaattaacg aaaacgagta
    21301 tttgtatgcc cgtcgcgtcg ggaatcagct gggcctgcgt gagctgaata tctgcaccgg
    21361 ctgtggtccg ggagcgatgg aagcgccgat gaaaggtgct gcggtcggac acgcgcagca
    21421 gcgttacaaa gacagtcgct ttattggtat gacagagccg tcgattatcg ccgctgaacc
    21481 gcctaacccg ctggtcaacg aattgatcat catgccagat atcgaaaaac gtctggaagc
    21541 gtttgtccgt atcgctcacg gtatcattat cttccctggc ggtgtgggta cagcagaaga
    21601 gttgctgtat ttgctgggaa ttttaatgaa cccggccaac aaagatcagg ttttaccatt
    21661 gatcctcacc ggcccgaaag agagcgccga ctacttccgc gtactggacg agtttgtcgt
    21721 gcatacgctg ggtgaaaacg cgcgccgcca ttaccgcatc atcattgatg acgccgctga
    21781 agtcgctcgt cagatgaaaa aatcgatgcc gctggtgaaa gaaaatcgcc gcgacactgg
    21841 tgacgcttac agctttaact ggtcaatgcg cattgcgcca gatttgcaaa tgccgtttga
    21901 gccgtctcac gagaatatgg ctaatctgaa gctttacccg gatcaacccg ttgaagtgct
    21961 ggctgccgac ctgcgccgtg cgttctccgg tattgtggcg ggtaatgtaa aagaagtcgg
    22021 tattcgcgcc attgaagagt ttggtcctta caaaatcaac ggcgataaag agattatgcg
    22081 tcgtatggac gacctgctac agggttttgt tgcccagcat cgtatgaagt tgccaggctc
    22141 agcctacatc ccttgctacg aaatctgcac gtaatctccc cttccccggt gacgtttgtt
    22201 gccgggtttt ctcgtttttg gtcacttact catcaactca tttcatttgt tatatgaatg
    22261 tttcttacca ccctcacgcg acaaatatca ccacagttaa tatgtcatac aatttatgtt
    22321 gaaacgcaaa cgtttcccta ttttcataaa ccacatttta tcgccataaa aaagaaatta
    22381 tcgccatgat taactaaaag tattgatttt ttcagttcaa cctacatata ttgcgcgccc
    22441 cggaaaaagt cagatgtcgt ttaatgggca aatattgccc ttaaattctc ttttactttt
    22501 gatttacaga gtaaagcgtt gggattatct atcttccaag tagattattg tatttgggat
    22561 caagatcact gatagataca taacttgtgt gtatctttcc gccctcaaat tattacggtg
    22621 gaaaatgatt aagccatcgc cgatagacag atttcatttt tacggtcagg caccttcccg
    22681 ggctgaactg gctaaaagct gaattatttg cattcctcca ggagaaatag atggaaacga
    22741 ctcaaaccag cacgattgcg tcgaaagact ctcgtagtgc ctggcgcaag acagacacca
    22801 tgtggatgct gggcctttac ggcacggcaa tcggcgcggg cgtgctgttc ctgccaatca
    22861 acgctggtgt tggcggtatg atcccgctga tcatcatggc tatccttgcg ttcccgatga
    22921 cgttttttgc tcaccgcggc ctgactcgct tcgtactgtc tggtaaaaac ccgggcgaag
    22981 acatcaccga ggttgtagaa gaacactttg gtattggcgc aggtaaactg attaccctgc
    23041 tctacttctt cgctatctac ccgatcctgc tggtttatag cgtggcaatc accaatacag
    23101 ttgaaagctt catgtctcac cagctgggta tgacgccacc gccgcgtgcg attctgtcgc
    23161 tgatcctgat cgtgggtatg atgaccatcg ttcgcttcgg tgagcagatg atcgttaaag
    23221 cgatgagcat tctggtattc ccgtttgttg gcgtactgat gctgctggct ctgtacctga
    23281 tcccgcagtg gaacggcgca gcactggaaa cgctgtctct ggacactgca tctgcaaccg
    23341 gaaacggtct gtggatgacc ctgtggctgg caattccggt aatggtgttc tcgttcaacc
    23401 actctccgat catctcttct ttcgccgttg cgaagcgtga agagtacggc gatatggcag
    23461 aacagaaatg ctctaagatc ctggcattcg cacacatcat gatggtgctg accgtaatgt
    23521 tcttcgtctt cagctgcgta ctgagcctga ctccggcaga cctggctgcg gctaaagagc
    23581 agaacatctc gattctgtct tacctggcta accactttaa cgcgccgatc atcgcgtgga
    23641 tggctccgat tatcgcgatt atcgctatca ccaaatcctt cctcggccac tacctgggcg
    23701 cacgtgaagg cttcaacggt atggtgatta aatctctgcg tggtaaaggt aagtctatcg
    23761 aaatcaacaa gctgaaccgt atcactgcgc tgttcatgct ggtaacgacc tggattgttg
    23821 ccaccctgaa cccgagcatc ctgggtatga ttgaaaccct gggcggccca atcatcgcga
    23881 tgatcctgtt cctgatgccg atgtacgcaa ttcagaaagt accggcaatg cgtaagtaca
    23941 gcggtcacat cagcaacgta ttcgttgtcg tgatgggtct gattgcaatc tccgcaatct
    24001 tctactctct gttcagctaa gtcctttcgc gccgctttcg ggcggcgctt cctccgtttt
    24061 aacgcgatgt atttcctatg attagcgtat tcgatatttt caaaatcggc attggccctt
    24121 ccagttctca taccgttgga ccaatgaaag cgggtaaaca atttaccgac gatctgattg
    24181 cccgtaacct gcttaaagac gtgacccgcg tggtggttga cgtgtacggc tcgctctctc
    24241 tgactggtaa aggccaccac actgatatcg ccattattat gggcctggcg ggtaacctgc
    24301 cggataccgt ggatatcgat tccatccctg gttttattca ggatgtgaat actcatggtc
    24361 gcctgatgct ggcaaacggt cagcatgaag tggagttccc ggttgatcag tgcatgaact
    24421 tccatgccga caacctttct ctgcatgaaa acggtatgcg cattaccgcg ctggcgggcg
    24481 ataaagtcgt ttacagccag acttactact ctatcggcgg tggctttatc gttgatgaag
    24541 aacattttgg ccagcaggat agcgcaccgg ttgaagttcc ttatccgtac agttcagcag
    24601 ccgatctgca aaaacattgt caggaaaccg ggctgtcact ctctggcctg atgatgaaaa
    24661 acgagctggc gctgcacagc aaagaagagc tggaacagca cctggcgaac gtctgggaag
    24721 tcatgcgtgg cggtattgag cgcggtattt ccaccgaagg cgtgttgcct ggcaaactgc
    24781 gcgttccacg ccgtgctgcg gcactacgcc ggatgctggt cagccaggat aaaaccacca
    24841 ctgacccgat ggcggttgtt gactggatca acatgtttgc actggcagtg aacgaagaga
    24901 acgctgctgg cggtcgcgtg gtgactgcgc cgactaacgg tgcgtgcggg attatcccgg
    24961 ccgtgctggc gtactacgac aagtttatcc gcgaagtgaa cgctaactca ctggctcgtt
    25021 acctgctggt agccagcgcc attggttctc tttataagat gaacgcctcg atttctggtg
    25081 ccgaagtcgg ctgccagggt gaagtcggcg tggcgtgctc aatggcggca gctggtctgg
    25141 cagaactgtt aggcgcaagc ccggcgcagg tatgcatcgc ggcggaaatc gccatggagc
    25201 acaacctcgg tctgacgtgt gacccggttg ccggacaggt ccaggtaccg tgcatcgagc
    25261 gtaacgccat tgcagcagta aaagcggtga atgctgcacg tatggcgctg cgccgtacca
    25321 gcgagccgcg cgtctgcctc gataaagtta tcgaaactat gtacgaaaca ggtaaagata
    25381 tgaacgccaa gtaccgcgaa acctctcgcg gcggcctggc aatgaagatc gttgcctgcg
    25441 attaatcgct ctccagaggc ctctttttgc gaggcctctt cccgatttct catccagcca
    25501 tagcctgttc tggcatcgaa tgttaccctt atcacctgat ctttaagggg gttatcgtgg
    25561 ctgttcattt gcttattgtc gatgcactga atcttattcg tcgcattcat gccgttcagg
    25621 gttcgccctg tgtcgaaacc tgccagcacg cgctcgatca gctcattatg cacagccagc
    25681 caacccacgc ggtcgccgtt tttgatgatg aaaaccgcag tagcggctgg cgtcatcagc
    25741 gtttaccaga ttacaaagcg ggtcgaccgc caatgccgga agagttgcac aacgagatgc
    25801 ctgcattacg cgccgccttt gagcaacgcg gcgtcccgtg ctggtccgcc agcggcaacg
    25861 aagccgatga cttagccgcc acgctggcgg tcaaagtgac acaggccggg catcaggcaa
    25921 cgattgtttc gacagataaa ggctactgtc agttactttc accgacatta cgtattcgtg
    25981 attacttcca gaaacgttgg ctggatgcgc catttatcga taaagaattt ggcgttcaac
    26041 cgcagcagtt gcccgattac tgggggcttg cggggatcag cagttcaaag gtaccgggtg
    26101 ttgcagggat cggaccaaaa agcgccacac agctactggt cgagtttcag agtctggaag
    26161 ggatatatga gaatctggat gcggttgccg aaaagtggcg caaaaaatta gaaaaccata
    26221 aagagatggc gtttctgtgc cgcgatattg cccgcttaca aaccgatttg catatcgacg
    26281 gcaatttaca gcaattgcgg ttggtaaggt aacggcgtgc cggatgcaaa cgattatcaa
    26341 cacattgaat ttaatgcact tatatgccgg ataagacgcg gctagcgtcg catccggcat
    26401 tatcacatcc gcgcaattac caggcggtat ggtaaagctc tacaatatcc tcaagcgttg
    26461 cttcacgcgg gttgccaccg gtacaaacat catccagtgc cgcctgcgcc agtgccggaa
    26521 tgtcttcctt gcgtacacca acatcacgta aatgtggcgg aataccgaca tcacggttga
    26581 gtgcaaacac cgcttcaaca gcggcattac gcgcctcttc caggctcata ccttccactt
    26641 tcacgcccat aacgcgcgcg atatcgcggt acttctcacc ggtaaagtca gcgttatagc
    26701 gcatgacatg cggtaacagg atggcatttg caacaccgtg tggcgtgttg taaaacgcgc
    26761 ccagtggatg cgccatacca tgcaccaacc ctaacccaac attcgagaat cccatacccg
    26821 caacatactg cccgagcgcc atttcttctc cggcatcctt atcaccagca accgatcctc
    26881 gcagcgcccc agcaatgatt tcaatcgctt taatgtgcag cgcatcggtt agcgcccacg
    26941 cgccacgggt aatatacccc tcaatagcat gagtgagcgc atcgacaccc gtcgcggctt
    27001 tcagcgctgg aggcatacca tccatcatgt cagcgtcaat aaacgccacc tgcgggatat
    27061 catgcggatc aacgcaaaca aacttgcgcc gtttctcttc gtcagtgatc acgtagttaa
    27121 tggtcacttc tgccgcagtg cctgctgtgg tggggattgc cagaatcggt acactgggtt
    27181 tattggtcgg ggaaagccct tccaggctac gcacatcggc aaactccggg ttgttgctga
    27241 taatgccaat cgctttacac gtatcctgtg gagaaccacc accaatagcg atcaggtaat
    27301 ccgcgccgct attctggaat acatcgagcc cttctttgac gacagtaatt gttgggttag
    27361 gtactacgcc gtcgtaaatc gcccatgcca gtcctgccgc atccatctta tcgatcactt
    27421 tcgccaccac gccgcattgc accagcgttt tatcggtgac gatcagcgcc ttctgataac
    27481 cacggcgttt cacttcatcg gttaaagctc caacagcacc tcgaccaaac catgccgttt
    27541 cgttcagaat cattctgtta gccatcatcc ttctccttgt tgctttacga aattactctt
    27601 caatccgtaa cccgtaggtt ttgaatttct ccagcactac ggcaatctct tcatcgctca
    27661 gcactggcac cgggtccgta atcgccaggg tcgtcaggta aagttgtgcc agcacttcaa
    27721 cttcatgcgc caaccataac gctttctcca gattcgcctc acaagcgata agcccatgat
    27781 gttgtaacaa agttgcctta cgatttttga gagccagcgc aacatgttca gaaagttcgc
    27841 gtgttccaaa ggtcgcataa ggcgcgcaag gaatagaatt accgccagcc gccgcaatca
    27901 tgtagtgaat cgcggggatc ggtcggttaa gaatggaaac tgccgtacaa tgaacggcat
    27961 gattgtgaac aaccgcgttg gcatccggtc tgctttgata ggccgccata tggaaacgcc
    28021 actcgcttga ggggagtttt ccttcctcat gtttgccgtt gccatcaata aagacaatat
    28081 gcgactccgt cagtttttca tatggaatac ctgtaggcgt aatcagcatc ccatcctgat
    28141 aacgtacact gacgttaccc gctgtcccct ggttcagccc caggcgagtc atttccaggc
    28201 aagtgtcaat aatctgacga gcaagtttat ttcgttccat tagctacctc tctctgattc
    28261 aaaacagggc aataatgttg ttcctttcac actattgaat tagccgttta attactcacc
    28321 atattcttcc tgattaacca gaaagaaatt cacaagctta tattttgtga cctggttcaa
    28381 ctaatcacag taaataactg cattttctct ttttataacc ccattaaaaa tgaccgctct
    28441 ta
//
LOCUS       DAZFJL010000053        28347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFJL010000053 DAZFJL010000000
VERSION     DAZFJL010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.053"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(44..193)
                     /locus_tag="ACHJ8C_002218"
     CDS             complement(44..193)
                     /locus_tag="ACHJ8C_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5552070.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            502..1641
                     /gene="wza"
                     /locus_tag="ACHJ8C_002219"
     CDS             502..1641
                     /gene="wza"
                     /locus_tag="ACHJ8C_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO5552071.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGESGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1647..2090
                     /gene="wzb"
                     /locus_tag="ACHJ8C_002220"
     CDS             1647..2090
                     /gene="wzb"
                     /locus_tag="ACHJ8C_002220"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO5552072.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSQETFTAVYALLERSARQWAQALNAEQV"
     gene            2093..4255
                     /gene="wzc"
                     /locus_tag="ACHJ8C_002221"
     CDS             2093..4255
                     /gene="wzc"
                     /locus_tag="ACHJ8C_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO5552073.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTIIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMVDQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4348..5187
                     /gene="wcaA"
                     /locus_tag="ACHJ8C_002222"
     CDS             4348..5187
                     /gene="wcaA"
                     /locus_tag="ACHJ8C_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO5552074.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5190..5678
                     /gene="wcaB"
                     /locus_tag="ACHJ8C_002223"
     CDS             5190..5678
                     /gene="wcaB"
                     /locus_tag="ACHJ8C_002223"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO5552075.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITIGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5675..6892
                     /gene="wcaC"
                     /locus_tag="ACHJ8C_002224"
     CDS             5675..6892
                     /gene="wcaC"
                     /locus_tag="ACHJ8C_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO5552076.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLLNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLSEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6867..8084
                     /gene="wcaD"
                     /locus_tag="ACHJ8C_002225"
     CDS             6867..8084
                     /gene="wcaD"
                     /locus_tag="ACHJ8C_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO5552077.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVVLAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8095..8841
                     /gene="wcaE"
                     /locus_tag="ACHJ8C_002226"
     CDS             8095..8841
                     /gene="wcaE"
                     /locus_tag="ACHJ8C_002226"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO5552078.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLKGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHRDAANF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTINNMELCADAKKVQWQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8857..9405
                     /gene="wcaF"
                     /locus_tag="ACHJ8C_002227"
     CDS             8857..9405
                     /gene="wcaF"
                     /locus_tag="ACHJ8C_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO5552079.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRKRVETE"
     gene            9432..10553
                     /gene="gmd"
                     /locus_tag="ACHJ8C_002228"
     CDS             9432..10553
                     /gene="gmd"
                     /locus_tag="ACHJ8C_002228"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO5552080.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEVAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10556..11521
                     /gene="fcl"
                     /locus_tag="ACHJ8C_002229"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10556..11521
                     /gene="fcl"
                     /locus_tag="ACHJ8C_002229"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO5552081.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11524..12003
                     /gene="gmm"
                     /locus_tag="ACHJ8C_002230"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11524..12003
                     /gene="gmm"
                     /locus_tag="ACHJ8C_002230"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO5552082.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVSEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            12000..13223
                     /gene="wcaI"
                     /locus_tag="ACHJ8C_002231"
     CDS             12000..13223
                     /gene="wcaI"
                     /locus_tag="ACHJ8C_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO5552083.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVANADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13226..14662
                     /gene="cpsB"
                     /locus_tag="ACHJ8C_002232"
     CDS             13226..14662
                     /gene="cpsB"
                     /locus_tag="ACHJ8C_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO5552084.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14791..16161
                     /gene="cpsG"
                     /locus_tag="ACHJ8C_002233"
                     /gene_synonym="manB"
     CDS             14791..16161
                     /gene="cpsG"
                     /locus_tag="ACHJ8C_002233"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO5552085.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVKRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16216..17610
                     /gene="wcaJ"
                     /locus_tag="ACHJ8C_002234"
     CDS             16216..17610
                     /gene="wcaJ"
                     /locus_tag="ACHJ8C_002234"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO5552086.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     FYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSCIEEMNGVPVVPLYDTPLSGVNRLIKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17612..19090
                     /gene="wzxC"
                     /locus_tag="ACHJ8C_002235"
     CDS             17612..19090
                     /gene="wzxC"
                     /locus_tag="ACHJ8C_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO5552087.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRINFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWSSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQISAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19560..20840
                     /gene="wcaK"
                     /locus_tag="ACHJ8C_002236"
     CDS             19560..20840
                     /gene="wcaK"
                     /locus_tag="ACHJ8C_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO5552088.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGHVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDTIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHIEDF
                     TASYAVQHWLDVAAQQKAVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFSTPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVKSVLERIGEAK"
     gene            20837..22057
                     /gene="wcaL"
                     /locus_tag="ACHJ8C_002237"
     CDS             20837..22057
                     /gene="wcaL"
                     /locus_tag="ACHJ8C_002237"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO5552089.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFYADVFIAHFGPAGVIAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAILDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            22068..23462
                     /gene="wcaM"
                     /locus_tag="ACHJ8C_002238"
     CDS             22068..23462
                     /gene="wcaM"
                     /locus_tag="ACHJ8C_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO5552090.1"
                     /translation="MPFKKLSRRNFLTASSVLAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPSGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLTMTGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPENQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPNFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNALSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIV
                     NVEKINFRLPERRE"
     gene            23637..24530
                     /gene="galF"
                     /locus_tag="ACHJ8C_002239"
     CDS             23637..24530
                     /gene="galF"
                     /locus_tag="ACHJ8C_002239"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO5552091.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSLIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            24903..25988
                     /gene="rfbB"
                     /locus_tag="ACHJ8C_002240"
     CDS             24903..25988
                     /gene="rfbB"
                     /locus_tag="ACHJ8C_002240"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO5552092.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KGLPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            25988..26887
                     /gene="rfbD"
                     /locus_tag="ACHJ8C_002241"
     CDS             25988..26887
                     /gene="rfbD"
                     /locus_tag="ACHJ8C_002241"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFO5552093.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALKKPEVAGLYHLVAS
                     GTTTWYDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            26945..27823
                     /gene="rfbA"
                     /locus_tag="ACHJ8C_002242"
     CDS             26945..27823
                     /gene="rfbA"
                     /locus_tag="ACHJ8C_002242"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO5552094.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKRFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            27828..>28347
                     /gene="rfbC"
                     /locus_tag="ACHJ8C_002243"
     CDS             27828..>28347
                     /gene="rfbC"
                     /locus_tag="ACHJ8C_002243"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFO5552095.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSCKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFLYKTTNYYAPIYEKSILWSDNKINIAWPLQ
                     GGFILSDKDRNAS"
BASE COUNT         7089 a         6829 c         7618 g         6811 t
ORIGIN      
        1 tatttaagaa atatcgcata tggagacgct ataataaata ttgtcaacct aaagaaactc
       61 ctaaaaacca tattgaatga cacttaatat aattcttaaa aatagccaat tgccgaattg
      121 ttaccttgcc tgctattccg ttagctgtaa cacttcctcc tgcattattg gagagccaat
      181 attcaattac cataatgcca ccttatattc ggaaattaat ttccgcaata accctttctc
      241 atcaacgact gtatgcagag agggtttgaa aaattaaact aaatcagtgt attggtagct
      301 aaaaagccag gggcggtagc gtgtctggat gcctgaaaga ccagtctgaa ttattctgtc
      361 aatagcctgc ggatgaaatc aattttttta ggactgatac cagttaaatt tttattcaat
      421 taactgcaag gtaaatgtgc atggcgacat tgctattaat agtgcacagg ataattactc
      481 tgccaaagtg ataaataaac aatgatgaaa tccaaaatga aattgatgcc attattggtg
      541 tcagtaacct tgataagcgg ttgcacagta cttccgggca gcaatatgtc gacgatgggg
      601 aaagacgtca tcaaacagca ggacgctgat ttcgacctcg acaaaatggt gaatgtttat
      661 ccgttaaccc cgcgcctgat tgatcaatta cgcccacgac ccaacgtagc gcgccccaat
      721 atgacgctgg aaagtgagat cgcgaattac cagtatcgcg tcgggccggg ggacgttctt
      781 aatgtcaccg tctgggatca cccggaactc accacgccag ctggtcagta ccgcagctcc
      841 agcgacaccg gcaactgggt acagccagac ggcaccatgt tctacccgta tatcggcaag
      901 gttcacgtcg tggggaaaac cctcgctgaa atccgcagtg atattaccgg gcgcttagcg
      961 acgtatatcg ctgacccgca ggtggacgtt aatatcgcag cattccgctc gcaaaaggcc
     1021 tatatctccg gtcaggtgaa taaatccggt caacaggcga tcaccaacgt gccactgacc
     1081 attctcgacg ctatcaacgc cgcaggtggc ctgaccgaca ccgctgactg gcgcaacgtg
     1141 gtgctaacac acaatggtcg tgaagagcgc atttctttgc aggcgctgat gcaaaacggc
     1201 gacctcaatc agaaccgact gctttacccc ggcgatattc tttacgtgcc acgtaatgat
     1261 gatctgaaag tgtttgtgat gggtgaagtg aagaaacaga gcaccctgaa aatggacttt
     1321 agcggcatga ccctgactga agccctgggc aatgctgaag gcatcgacat gaccacctcc
     1381 aacgccagcg gcatctttgt cattcgtccg ctgaaaggcg agagcgggcg taacggcaag
     1441 attgccaata tctaccagct ggatatgtcc gatgccacgt cgctggtgat ggcgacagaa
     1501 ttccgcctgc aaccttatga cgtggtgtat gtcaccaccg ccccggtttc ccgctggaac
     1561 cgtctgatca atcagttgct gccaactatt agcggtgtcc gttacatgac ggatacagcc
     1621 agcgacattc ataactggta atcgtcatgt ttaacaacat cttagttgtc tgtgtcggca
     1681 atatttgccg ttccccgacg gcggaacgct tactgcaacg ttatcacccg gagctaaaag
     1741 tggagtccgc tggactcggc gcgctggtcg gtaagggcgc tgatcctacc gccatcagcg
     1801 tcgccgcaga acatcaactg tctctggaag gtcattgcgc acggcaaatc agccgccgtc
     1861 tgtgtcgcaa ctacgacctg attttgacca tggaaaagcg ccatatcgaa cgcttatgcg
     1921 agatggcacc agagatgcgc ggcaaagtga tgctgtttgg tcactgggat aacgaatgtg
     1981 aaatccccga tccgtatcgc aaaagccagg aaacgtttac agcggtgtac gcattacttg
     2041 aacggtctgc ccgccagtgg gcgcaggcat tgaacgcaga gcaggtataa gaatgacaga
     2101 aaaagtaaaa caacatgccg ctccggtaac gggcagtgat gaaatcgata ttggtcgcct
     2161 ggttggcacc atcattgaag cacgctggtg ggtgattggc attaccgccg tattcgccct
     2221 ctgtgccgtg gtttacacct tcttcgctac gccgatatac agcgccgacg cactggtaca
     2281 aatcgaacaa agcagcggca attcgttagt gcaggatatc ggatcggcat tagccaacaa
     2341 accgcctgca tcggacgccg agatccagtt gattcgttcg cgtctggtgc ttggtaaaac
     2401 ggtggacgat ctcgacctcg atattgcagt gagcaaaaac acgttcccga ttttcggtgc
     2461 gggctgggag cgcctgatgg gacgtcagaa cgagacggta aaagtgacca cctttaaccg
     2521 tccaaaagag atggtggatc aggtgtttac gcttaatgtg ctggacaaca aaaactacac
     2581 tttgagcagc gacggcggct ttagcgctcg tgggcaagcg ggtcagatgc tgaaaaaaga
     2641 aggcgtcacg ctgatggttg aagccattca cgccagcccg ggcagtgagt ttaccgtcac
     2701 taaatactcc acgctgggga tgatcaatca actgcaaaac agcctgacgg taacagagaa
     2761 tggcaaagac gctggcgtac tgagcctgac ttataccggt gaagatcgcg aacagatccg
     2821 cgacattctt aacagcatcg cccgtaacta tcaggagcaa aatattgagc gcaaatcggc
     2881 ggaagcgtcg aaaagcctcg ccttcctcgc gcaacagtta ccggaagtac gtagccgcct
     2941 tgatgttgcc gaaaacaaac tgaatgcctt ccgtcaggat aaagattctg ttgatctgcc
     3001 gctggaagca aaagcggtgc tcgattcgat ggtgaacatc gacgcgcagt tgaacgaact
     3061 gacctttaaa gaggcggaaa tctccaagct gtacaccaaa gttcatccgg cgtaccgcac
     3121 gctgctggag aaacggcagg cgctggaaga cgaaaaagcc aaacttaatg gtcgcgtaac
     3181 ggcgatgccg aaaacccagc aggaaattgt ccgtctgacc cgcgatgtcg agtctgggca
     3241 gcaggtctat atgcaactgc tgaataaaga acaggagctg aaaatcaccg aagccagcac
     3301 cgtcggcgat gtgcgcattg ttgacccggc aatcacccag cctggcgtgc taaaaccgaa
     3361 gaaagggctg attatcctcg gggcgattat ccttggcctg atgctctcta tcgtgggtgt
     3421 gctgctgcgc tcgttgttta atcgcggcat cgaaagcccg caggtgctgg aagaacacgg
     3481 tatcagtgtc tatgccagca tcccgctgtc ggagtggcag aaagcgcgcg atagcgtcaa
     3541 aaccatcaaa gggattaaac gctataaaca gagccagcta ctggcggtgg ggaatccaac
     3601 cgatctggcg attgaagcca tccgcagtct gcgtaccagt ttgcacttcg cgatgatgca
     3661 ggcacagaac aatgtgttga tgatgaccgg ggttagcccg tcaatcggta aaacctttgt
     3721 ctgcgccaac ctggcggcgg tgattagcca gaccaataaa cgggtgttgt tgatcgactg
     3781 cgatatgcgc aaaggttaca cccacgagct gttgggcact aataacgtta atggcctgtc
     3841 ggaaattctg attggacagg gcgatattac taccgcggct aaaccgacct ctattgccaa
     3901 atttgacctg atcccgcgcg gtcaggtacc gccaaatcct tctgaactgt tgatgagcga
     3961 acgctttgcc gaactggtga actgggcgag taaaaactac gacctggtgt tgattgatac
     4021 gccgccgatt ctggcagtga ccgatgcggc aattgttggt cgtcatgtcg gaaccacgtt
     4081 aatggtggcg cgttatgcgg tcaacacatt gaaagaagtg gaaaccagtc tgagccgctt
     4141 tgagcaaaac ggtattccgg tgaaaggggt gattctgaac tccatcttcc gccgcgccag
     4201 cgcgtatcag gattatggct attacgaata cgaatataag tcggatgcga aataacgacg
     4261 cttgcattcg caccgccccg taggccggat aaggcgttca cgccgcatcc ggcaagcaaa
     4321 ccagctcata agccgggaga acaacctatg aaagacaatc cgctgatttc aatctatatg
     4381 ccgacctgga accgccaaca actggcgatt cgggcgataa aatcggtcct gcgccaggac
     4441 tacagtaact gggagatgat tatcgtggat gattgctcca cctcctggga acaactacaa
     4501 cagtacgtca ccgccctcaa cgatccgcgc atcacttaca ttcacaacga cattaacagc
     4561 ggggcgtgcg cggtacgtaa ccaggcgatt atgctggcgc aaggggaata catcaccggg
     4621 atcgatgacg acgatgaatg gacacccaac cgcctgagcg tcttcctcgc ccataaacag
     4681 caactggtca ctcacgcctt tttgtacgct aacgactacg tttgccaggg cgaagtctat
     4741 tcccaaccgg caagtttgcc gctgtatccc aaatcgccgt attcacgccg cctgttttac
     4801 aaacgcaata tcattggcaa tcaggtcttt acctgggcgt ggcgtttcaa agagtgcttg
     4861 ttcgataccg aactgaaagc ggcacaggat tacgacatct tcctgcggat ggtggtggag
     4921 tacggcgaac cgtggaaagt ggaagaggcg acgcagatcc tgcatattaa tcacggcgaa
     4981 atgcagatca cctcgtcacc gaaaaaattc tccggatact tccactttta ccgcaagcac
     5041 aaagacaaat tcgaccgcgc cagcaaaaaa tatcagctgt ttaccctcta ccagatccgc
     5101 aataaacgca tgacctggcg cactttgctg acgctgctgt cggtgcgtaa cggcaaacgt
     5161 ctggctgatg gtattcgggg gcgctaaaga tgctggaaga tctgcgcgcc aacagctgga
     5221 gtttacgccc gtgctgcatg gttctcgcct atcgcgtcgc tcatttttgt tcggtgtggc
     5281 gcaaaaagaa cgtcctcaac aatctgtggg cggccccgct gctggtgctg tatcgcatta
     5341 tcaccgaatg ctttttcggt tatgaaatcc aggccgccgc gaccattggc cgccgcttta
     5401 ctatccatca cggttacgcc gtagtgatca ataaaaacgt ggtcgcgggg gatgatttca
     5461 ccattcgtca cggcgtcact atcggcaatc gtggtgccga taacatggca tgtccacaca
     5521 ttggcaacgg cgtcgaactc ggtgccaacg tcattattct tggtgatatc acgattggta
     5581 acaacgtcac cgtgggcgcg ggcagcgtgg tgctcgactc tgtcccggac aacgcgctgg
     5641 tggtgggaga aaaagcgcga gtgaaggtaa ttaaatgaat attctgcaat ttaatgtgcg
     5701 actggcggaa ggcggggcag caggtgtggc gttagatctc caccagcgcg cgctgcaaca
     5761 ggggctggcg tcacattttg tctacggcta cggcaaaggc ggcaaagaga gcgtcagcca
     5821 tcagaactat ccgcaggtca tcaaacatac gccacggatg accgcgatgg cgaacattgc
     5881 gttgtttcgt ctgcttaatc gcgatctgtt cggcaatttc aatgagttat atcgcaccat
     5941 tactcgcaca ccgggcccgg tggttctgca ttttcatgtg ctgcacagct actggttgaa
     6001 tcttaacagc gtggtgcgct tttgcgaaaa agtgaaaaac cataaaccgg atgtcactct
     6061 ggtctggacg ctacacgacc attggagcgt taccggacgc tgcgccttta ccgacggttg
     6121 cgaaggctgg aaaacgggct gccagaaatg cccgacctta aataactatc caccggtgaa
     6181 gattgatcgc gcgcaccagc tggtggcggg taaacgccag ttattccgcg agatgctggc
     6241 actgggctgt cagtttattt cccccagcca gcatgtggct gacgctttca atagcctgta
     6301 cggtccaggg cgttgccgga ttatcaataa cggtattgat atggcaaccg aagcgattct
     6361 ggcggacttg cctccggtgc gcgaaaccca gggcaagccg aaaatcgcgg tggtggcgca
     6421 tgacctgcgt tacgacggta agactaacca gcaactggtg cgtgagatga tggcgctggg
     6481 cgacaaaatt gaactgcata cctttggtaa gttctcgccg ttcaccgctg gcaacgtggt
     6541 taatcacggc tttgaaactg acaagcgcaa gttgatgagc gcgctcaatc agatggatgc
     6601 gctggtattc agttctcgcg tcgataacta cccgctgatt ttgtgtgagg cgctatcgat
     6661 tggcgtgccg gtgattgcca cccatagcga cgcggcgcgt gaagtgttgc aaaaatccgg
     6721 cggtaaaacc gtcagcgaag aagaggtgct gcaactggtg cagttaagca aaccggaaat
     6781 cgcgcaggcg atatttggta ccacgctgtc tgagttcagc caacgcagcc gcgccgccta
     6841 cagtggacaa cagatgctgg aggagtatgt caacttctat cagaatctgt agctacctgc
     6901 tgctgccgct gatctacctg ctggttaacg tcaaaatcgc ccagcttggc gaaagtttcc
     6961 ccatcaccat cgtcactttt ttacctgttt tgctgctgct gtttttagag cgcatcagcg
     7021 ttaaaaaatt gatgattgcc ctggggattg gcgcgggact cacggcgttt aactatctgt
     7081 ttggtcagtc tttggatgcc agtaaatacg tcacttcaac catgctgttt gtctatattg
     7141 tgatcattat tggcatggtg tggagtattc gctttaaaac gatttcgcca cacaaccatc
     7201 gcaagatatt acgtttcttt tatctggtgg tcgggctggt ggtggtgctg gcggcggtgg
     7261 agatggcgca aattatcctc accggcggta gcagtattat ggagtcgatt tcgaaatatc
     7321 tgatttacag caacagctat gtgctgaatt tcattaaatt cggcggcaag cgcacgacag
     7381 cactttattt cgaaccggca tttttcgctc tggcattaat ctcaatttgg ctcagcatca
     7441 aacagtttgg tatcaaaacg cctaaaacag atgctatgat tctcgcaggg ataatattat
     7501 ccggatcgtt ttcaggggtt atgaccttta tcctttttta tttgctggag tgggcatttc
     7561 aatatctgaa taaagaggcg attaagaaaa agttaccgtt agcattgatt tctctggctg
     7621 tattcctggt tggtgtggta atcgcgttcc cttatatttc cacccgtctg ggcgatttag
     7681 gtacagaagg atcgtcatcg tattatcgta ttgtcggtcc gctggtgatg gtcggttatt
     7741 ctttaaccca tattgacggt gtagtcagat ttggctcact ttatgaatat gtcgcatcat
     7801 tcggaatatt taacggtgcg gatgtcggaa aaaccataga caatggtttg tatctgctga
     7861 ttatttattt ttcctggttc gcggtgtttt tatcgctgtg gtacatgggg aaagtgataa
     7921 aaatgatgat caacgctttt ggtgataacc gtaattttcg cgtgcaatta tatcttttta
     7981 ctccggtatc gctgtttttt accggttcga tatttagccc ggaatatgca tttttaatcg
     8041 tctgtccgtt tattttgcga aaagcgttaa atattacgag gtaagaataa gaacatgttg
     8101 cttagcataa tcactgtcgc gtttcgtaac ctcgaaggga tagtcaaaac acatgcctcg
     8161 ctggcgcatc tggcgcaggc ggacgatatc agcttcgaat ggattgttgt cgatggcggt
     8221 tccaacgacg gtacccgtga gtatctggaa aatctcaagg gtatctataa cctacgcttt
     8281 gtcagcgagc cagataacgg tatctacgac gctatgaaca aaggcattgc gatggcacaa
     8341 ggcaagttcg cgttgtttct caactcgggc gatatttttc atcgggatgc cgcaaatttt
     8401 gtccgtaagt taaaaatgca aaaagataac gtgatgatca ccggcgatgc gctgctggat
     8461 tttggcgacg ggcataaaat taaacgtagc gccaaaccgg gctggtatat ttaccacagc
     8521 ctgcccgcca gtcatcaggc gatatttttc ccggtgtccg gcttgaagaa atggcgttat
     8581 gacctggaat ataaagtttc ttccgattac gcgctggcgg ccaaaatgta taaagccggt
     8641 tatgcattta aaaaactcaa tggcctggtg tctgaatttt ctatgggtgg ggtatcaacc
     8701 atcaataata tggaattgtg tgctgacgcc aaaaaagtcc aatggcagat attgcacgtg
     8761 cctggctttt gggctgaatt atcctggcat ttacgccaac gtacgacctc aaagacgaaa
     8821 gccttatata acaaaagctg aatataaggg aaaacgatgc aggatttaag tggattctcg
     8881 gtgccgaaag ggttccgggg tggcaacgct attaaagtgc aattgtggtg ggcagtacag
     8941 gcaacaatat ttgcctggtc gccacaagta ttgtatcgct ggcgggcatt tttattacgt
     9001 ttattcggcg caaaaatagg aaaaaacgta gtcattcgtc cgtcagtaaa aattacctat
     9061 ccgtggaaat taacgttagg tgattacgcg tgggtcggcg atgaggtcaa tttatatacc
     9121 ctcggtgaaa taaccattgg cgcacattcg gtgatatcgc aaaaaagtta tttatgcact
     9181 ggtagccacg accatgcaag tcaacatttc accattaacg ccacgcctat tgtgattggc
     9241 gagaaatgct ggctggcaac cgatgtcttt gttgcccctg gcgtcacgat cggcgacggc
     9301 accgtcgtgg gtgcgcgaag cagtgttttt aaatcgcttc cggcaaatgt ggtttgtcgg
     9361 gggaaccccg cagtggtgat acgcaaacgc gttgaaactg aataaattca aaaaatacag
     9421 aggaataaga catgtcaaaa gtcgctctca tcaccggtgt aaccggacaa gacggttctt
     9481 acctggcaga gtttctgctg gaaaaaggtt acgaggtgca tggtattaag cgtcgtgcat
     9541 cgtcattcaa caccgagcgc gtggatcata tttatcagga tccgcacacc tgcaacccga
     9601 aattccatct gcattatggc gacctgagtg atacctccaa cctgacgcgc attttgcgtg
     9661 aagtgcagcc ggatgaagtg tacaacctgg gcgcaatgag ccacgttgcg gtttcttttg
     9721 agtcaccgga atataccgca gacgtcgatg cgatgggtac gctgcgcctg ctcgaggcga
     9781 tccgcttcct cggtctggaa aagaaaaccc gtttctatca ggcttccact tctgaactgt
     9841 acggtctggt gcaggaaatt ccgcagaaag agaccacgcc gttctacccg cgatctccgt
     9901 atgcggttgc caaactgtac gcctactgga tcaccgttaa ctaccgcgaa tcctacggca
     9961 tgtacgcctg taacggcatt ctctttaacc atgaatcccc gcgccgtggc gagactttcg
    10021 ttacccgcaa aatcacccgc gcaatcgcca acatcgccca ggggctggag tcgtgcctgt
    10081 atctcggcaa tatggattcc ctgcgtgact ggggccacgc caaagactac gtaaaaatgc
    10141 agtggatgat gctgcaacag gaacaaccgg aagatttcgt tattgccacc ggcgttcagt
    10201 actccgtgcg tcagttcgtg gaagtggcgg cggcacaact gggcatcaaa ctgcgctttg
    10261 aaggcacggg tgttgaagag aagggcattg tggtttccgt caccgggcat gacgcgccgg
    10321 gcgttaaacc gggtgatgtg attatcgccg ttgacccgcg ctacttccgt ccggctgaag
    10381 ttgaaacgct gctcggtgac ccgaccaaag cgcacgaaaa actgggctgg aaaccggaaa
    10441 tcaccctcag agagatggtg tctgaaatgg tggctaatga ccttgaagcg gcgaaaaaac
    10501 actctctgct gaaatctcac ggctacgacg tggcgatcgc gctggagtca taagcatgag
    10561 taaacaacgc atttttatcg ctggccatcg cgggatggtc ggttccgcca tcaggcggca
    10621 gctcgaacag cgcggtgatg tggagctggt attacgcacc cgcgatgaac tgaatctgct
    10681 ggacagccgc gcggtgcatg atttctttgc cagcgaacgt attgaccagg tctatctggc
    10741 ggcggcgaaa gtgggcggca ttgttgccaa caacacgtat ccggcagatt tcatctacca
    10801 gaacatgatg attgagagca acatcattca cgccgcgcat cagaacgacg tgaacaaact
    10861 gctgtttctc ggatcgtcct gtatctaccc gaaactggca aaacagccga tggcagaaag
    10921 cgagttattg cagggtacgc tggagccgac caacgagcct tacgccattg ccaaaatcgc
    10981 cgggatcaaa ctgtgtgaat cttacaaccg ccagtatgga cgcgattacc gctcggtcat
    11041 gccgaccaac ctgtatggcc cgcatgacaa cttccacccg agtaattcgc atgtgatccc
    11101 tgcattgctg cgtcgcttcc acgaggcgac ggcacagaat gcgccggacg tggtggtgtg
    11161 gggcagtggt acaccgatgc gcgaatttct gcatgtcgat gatatggcgg cagcgagcat
    11221 tcatgtgatg gagctggcgc acgaagtctg gctggagaac acccagccga tgctgtcgca
    11281 cattaacgtc ggcacgggcg ttgactgcac tatccgtgaa ctggcgcaaa ccatcgccaa
    11341 agtggtgggt tacaaaggtc gggtggtttt tgatgccagt aaaccggatg gtacgccgcg
    11401 caaactgctg gatgtgacgc gcctgcatca gcttggctgg tatcacgaaa tctcactgga
    11461 agcggggctt gccagcactt accagtggtt ccttgagaat caagaccgct ttcgggggta
    11521 atgatgtttt tacgtcagga agactttgcc acggtagtgc gctccactcc gcttgtctct
    11581 ctcgacttta ttgttgagaa cagtcgcggc gagtttctgc ttggcaaaag aaccaaccgc
    11641 ccggcgcagg gttactggtt tgtgccggga gggcgcgtgc agaaagacga aacgctggaa
    11701 gccgcatttg agcggctgac gatggcggaa ctggggctgc gtctgccgat aacagcaggc
    11761 cagttttacg gtgtctggca gcacttttat gacgataact tctccggcac ggatttcacc
    11821 actcactatg tggtgctcgg ttttcgcttc agagtatcgg aagaagagct gttactgccg
    11881 gatgagcagc atgacgatta ccgctggctg acgccggacg cgctgctcgc cagtaacgat
    11941 gttcacgcta acagccgcgc ctattttctc gctgagaagc gtgccggagt acccggatta
    12001 tgaaaatact ggtctacggc attaactact cgccggagtt aaccggcatc ggcaaataca
    12061 ccggagagat ggttgaatgg ctggcggcgc aaggtcatga ggtgcgggtt attaccgcac
    12121 cgccttacta cccgcagtgg caggtgggcg agaactattc cgcctggcgg tacaaacggg
    12181 aagagggggc cgccacggtg tggcgctgcc cgctgtacgt gccaaaacag ccgagcaccc
    12241 tgaaacgcct gttgcatctc ggcagttttg ccgtcagcag ttttttcccg ttgatggcgc
    12301 aacgtcgctg gaagccggat cgcattatcg gcgtagtgcc aacgctgttt tgcacgccag
    12361 gaatgcgcct gctggcgaaa ctgtctggtg cgcgcacggt gctccatatt caggattacg
    12421 aagtggacgc catgctgggg ctgggccttg ccggaaaagg taaaggcggc aaagtggcac
    12481 agctggcaac ggcgttcgaa cgtagcggac tgcataacgt cgataacgtc tccacgattt
    12541 cgcgttcgat gatgaataaa gctatcgaaa aaggcgtggc ggcggaaaac gtcatcttct
    12601 tccccaactg gtcggaaatc gcccgttttc agcatgttgc aaatgccgat gttgatgccc
    12661 ttcgtaacca gcttggcctg ccggataaca aaaaaatcat tctttactcc ggcaatattg
    12721 gtgaaaagca ggggctggaa aacgttattg aagctgccga tcgcctgcgc gatgaaccgc
    12781 tgatttttgc cattgtcggg cagggcggcg gcaaagcgcg gctggaaaaa atggcgcagc
    12841 agcgtggact gcgcaacatg caattttttc cgctgcaatc atatgacgct ttacccgcac
    12901 tgctgaagat gggggattgc catctggtgg tgcaaaaacg cggcgcggca gatgccgtat
    12961 tgccgtcgaa actgaccaat attctggcgg taggcggtaa cgcggtgatt accgcagaag
    13021 cccacacaga actgggacag ctttgcgaaa cctttccggg cattgcggtt tgcgtggaac
    13081 cggaatcggt cgaggcgctg gtggcgggga ttcgtcaggc gctcctgctg cccaaacaca
    13141 acacggtggc acgtgaatat gccgaacgca cgctcgataa agagaacgtg ttacgtcaat
    13201 ttataaatga tattcgggga taattatggc gcagtcgaaa ctctatccag ttgtgatggc
    13261 aggtggctcc ggtagccgct tatggccgct ttcccgcgta ctttacccca agcagttttt
    13321 atgcctgaaa ggcgatctca ccatgctgca aaccaccatc tgccgcctga acggcgtgga
    13381 gtgcgaaagc ccggtggtga tttgcaatga gcagcaccgc tttattgtcg cggaacagct
    13441 gcgtcaactg aacaaactca ccgagaacat tattctcgaa ccggcagggc gtaacactgc
    13501 accggccatt gcgctggcgg cgctggcggc aaaacgtcat agcccggaga acgacccatt
    13561 aatgctggtg ctggcggcgg atcatgtgat tgccgatgaa gacgcgttcc gtgccgccgt
    13621 gcgtaatgcc atgccgtatg ccgaagcggg caagctggtg accttcggca ttgtgccgga
    13681 tctacctgaa accggatatg gctatattcg tcgcggtgaa gtgtcggcgg gtgagcagga
    13741 tacggtggcc tttgaagtgg cgcagtttgt cgaaaaaccg aatctggaaa ccgcgcaggc
    13801 ctatgtggca agtggcgaat attactggaa cagcggtatg ttcctgttcc gtgccggacg
    13861 ttatctcgaa gaactgaaaa agtatcgtcc ggatattctc gatgcctgtg aaaaagcgat
    13921 gagcgccgtc gatccggatc tcgattttat tcgtgtggat gaagaggcgt ttctcgcctg
    13981 tccggaagag tcggtagatt acgcggtcat ggaacgcacg gcagatgccg ttgtggtacc
    14041 gatggatgcg ggctggagtg atgtcggttc ctggtcctct ttatgggaga tcagcgccca
    14101 caccgccgag ggcaacgttt gccatggcga tgtgattaat cacaaaactg aaaacagcta
    14161 tgtgtatgcc gaatctggcc tggtcaccac cgtcggggtg aaagatttgg tggtagtgca
    14221 gaccaaagat gcagtgctga ttgccgaccg taacgcggtg caggatgtga aaaaagtggt
    14281 cgagcagatc aaagctgatg gtcgccatga gcatcgggtg catcgcgaag tgtatcgtcc
    14341 gtggggcaaa tatgactcta tcgacgcggg cgaccgctac caggtgaaac gcatcaccgt
    14401 gaaaccgggc gaaggcttgt cggtacagat gcaccatcac cgcgcggaac actgggtggt
    14461 tgtcgcggga acggcaaaag tcactattga cggtgatatc aaactgcttg gtgaaaacga
    14521 gtccatttat attccgctgg gggcgacgca ttgcctggaa aacccgggga aaattccgct
    14581 cgatttaatt gaagtgcgct ccggctctta tctcgaagag gatgatgtgg tgcgcttcgc
    14641 ggatcgctac ggacgggtgt aaacgtcgca tcaggcgctg tttgtcggat gcggcgtgaa
    14701 cgccttatcc gaaccgcgat gtaaataacg acaaaaacaa aattggccgt ttcggtcagg
    14761 gccaactatt gcctgaaaaa gggtaacgac atgaaaaaat taacctgctt taaagcctac
    14821 gatattcgcg ggaaattagg cgaagaactg aatgaagata ttgcctggcg tattgggcgt
    14881 gcctatggcg aatttctcaa accgaaaacc attgtgttag gcggtgatgt ccgcctcacc
    14941 agcgaaacct taaaactggc gctggcgaaa ggtttacagg atgcgggcgt cgatgtgctg
    15001 gatattggca tgtccggcac cgaagagatc tattttgcca cgttccatct cggcgtggat
    15061 ggcggcatcg aagttaccgc cagccataac ccgatggatt acaacggcat gaagctggtg
    15121 cgcgaggggg ctcgcccgat cagcggtgat accggactgc gcgacgtcaa gcgtctggca
    15181 gaagccaacg acttcccccc cgtcgatgaa acaaaacgcg gtcgctatca gcaaatcaat
    15241 ctgcgtgacg cttacgttga tcacctgttc ggttatatca acgtcaaaaa cctcacgccg
    15301 ctcaagctgg tgattaactc cgggaacggc gcggcgggtc cggtggtgga cgccattgaa
    15361 gcccgcttta aagccctcgg cgcaccggtg gagttaatca aagtgcataa cacgccggac
    15421 ggcaatttcc ccaacggtat tcctaacccg ctactgccgg aatgtcgcga cgacacccgc
    15481 aatgcggtca tcaaacacgg cgcggatatg ggcattgctt ttgatggcga ttttgaccgc
    15541 tgtttcctgt ttgacgaaaa agggcagttt atcgagggtt actacattgt cggtttgctg
    15601 gcagaagcgt tcctcgaaaa aaatcccggc gcgaagatca tccacgatcc acgtctctcc
    15661 tggaacaccg ttgatgtggt gactgccgca ggcggcaccc cggtaatgtc gaaaaccgga
    15721 cacgccttta ttaaagaacg tatgcgcaag gaagacgcca tctacggtgg cgaaatgagc
    15781 gcccaccatt acttccgtga tttcgcttac tgcgacagcg gcatgatccc gtggctgctg
    15841 gtcgccgaac tggtgtgcct gaaaggaaaa acgctgggcg aactggtgcg cgaccggatg
    15901 gcggcattcc cggcaagcgg tgagatcaac agcaaactgg cgcaacccgt tgaggcgatt
    15961 aaccgcgtgg aacagcattt tagccgtgag gcgctggcgg tggatcgcac cgatggcatc
    16021 agcatgacct ttgccgactg gcgctttaat ctgcgctcct ccaacaccga accggtggtg
    16081 cggttgaatg tggaatcgcg cggcgatgta ccgctgatgg aagcgcgaac gcgaactctg
    16141 ctgacgttgc tgaacgagta atgtcggatc ttcccttacc ccactgcggg taaggggcta
    16201 ataacaggaa caacgatgac aaatctaaaa aagcgcgagc gagcgaaaac caatgcatcg
    16261 ttaatctcta tggtgcaacg cttttcagat atcaccatca tgtttgccgg actatggctg
    16321 gtttgcgaag tgagcggact gtcattcttc tacatgcacc tgttggtggc gctgattacg
    16381 ctggtggtgt tccagatgct gggcggcatc accgattttt atcgctcatg gcgcggtgtt
    16441 cgggcagcga cagaatttgc tctgttgcta caaaactgga cgttaagcgt aattttcagc
    16501 gccggactgg tggcgttcaa caatgatttc gacacgcaat tgaaaatctg gctggcgtgg
    16561 tatgggctga ccagtatcgg actggtggtt tgccgttcgt gtattcgcat tggggcgggc
    16621 tggctgcgta atcatggcta taacaagcgc atggtcgccg tagcggggga tttagccgcc
    16681 ggacaaatgc tgatggagag cttccgtaat cagccgtggt tagggtttga agtggtgggc
    16741 gtttaccacg acccgaaacc gggtggcgtt tctaacgact gggcgggcaa tctgcaacag
    16801 ctggtcgagg atgcgaaagc gggcaagatt cataacgtct atatcgcgat gcaaatgtgc
    16861 gacggcgcac gcgtgaaaaa actggtccat caactggcgg acaccacctg ttcggtgctg
    16921 ctgatccccg atgtctttac cttcaacatt ctccattcct gtatcgaaga gatgaacggc
    16981 gtaccggtgg tgccgctgta tgacacgccg ctttccgggg ttaaccgcct gataaaacgt
    17041 gcagaagaca ttgtgctggc gacgcttatt ctgctgctga tctccccggt gctgtgctgc
    17101 attgcgctgg cggtaaaact cagttcacct gggccggtta ttttccgcca gactcgctac
    17161 ggcatggatg gcaagccgat caaagtgtgg aagttccgtt ccatgaaagt gatggagaac
    17221 gacaaagtgg tgacccaggc gacgcagaac gatccgcgcg tcaccaaagt ggggaacttt
    17281 ctgcgccgca cctcgctgga tgaattgccg cagtttatca atgtgctgac tggcgggatg
    17341 tcgattgtcg gtccgcgtcc gcatgcggtg gcgcataacg aacaatatcg ccagctcatt
    17401 gaaggctaca tgctgcgcca taaagtgaaa ccgggcatta ccggttgggc gcagattaac
    17461 ggctggcgtg gcgaaaccga cacgctggag aaaatggaaa aacgcgtcga gttcgacctt
    17521 gagtacatcc gcgaatggag cgtctggttc gatatcaaaa tcgttttcct gacggtattc
    17581 aaaggtttcg ttaacaaagc ggcatattga tatgagctta cgtgaaaaaa ccatcagcgg
    17641 cgcgaagtgg tcggcgattg ccacggtgat catcatcggc ctcgggttgg tgcagatgac
    17701 cgtgctggcg cggattatcg acaaccacca gttcggtttg cttaccgtgt cgctggtgat
    17761 catcgcgctg gcagatacgc tttctgactt tggtatcgct aactcgatta ttcagcgaaa
    17821 agaaatcagt caccttgaac tcaccacgct gtactggctg aacgtcgggc tggggatcgt
    17881 ggtgtgcgtg gcggtgtttt tgttgagtga tgtcatcggt gacgtgttga ataacccgga
    17941 cctggcaccg ttgattaaaa cattgtcgct ggcgtttgtg gtgatccccc acgggcaaca
    18001 gttccgcgcg ttgatgcaaa aggagctgga gttcaacaaa atcggcatga tcgaaaccag
    18061 cgcggtgctg gcgggcttca cttttactgt ggttagcgcc catttctggc cgctggcgat
    18121 gaccgcgatc ctcggttatc tggtcaatag tgcggtaaga acgctgctgt ttggctactt
    18181 tggtcgaaaa atttatcgcc ccggtctgca tttctcgctg gcgtcggtgg caccgaactt
    18241 acgctttggt gcctggctga cggcggacag catcatcaac tatctcaaca ccaacctttc
    18301 aacgctggtg ctggcgcgta ttctcggcgc gggcgtggca gggggataca acctggcgta
    18361 caacgtggcc gttgtgccgc cgatgaagct gaacccaatc atcacccgcg tgttgtttcc
    18421 ggcatttgcc aaaattcagg acgacaccga aaagttgcgc attaacttct acaagctgct
    18481 gtcggtggtg gggattatca actttccggc actgctcggg ctaatggtgg tgtcgaataa
    18541 ttttgtaccg ctggtctttg gtgagaagtg gagcagcatt attccggtgc tgcaattgct
    18601 gtgtattgtg gggctgctgc gttcggtagg taacccgatt ggttcgctgc tgatggcgaa
    18661 agcgcgggtc gatatcagct ttaaattcaa cgtattcaaa acctttctgt ttattccggc
    18721 gattgttata ggtgggcaaa tggcgggcgc aatcggcgtc acgcttggct tcctgctggt
    18781 gcagattatc aacaccattc tgagttactt cgtgatgatt aaaccggttc ttggttccag
    18841 ttatcgccag tacatcctga gtttgtggct gccgttttat ctctcgctgc cgacgctggt
    18901 ggtcagttat gcgctgggca ttgtgctgaa agggcaactg gcgctgggga tgctgctggc
    18961 ggtgcaaata tccgcgggcg tgctggcgtt tgtggtgatg attgtgctgt cgcgccatcc
    19021 gttggtggtg gaagtaaagc gtcagttttg tcgcagcgaa aaaatgaaaa tgcttttacg
    19081 ggcggggtga atggttgttt tcggttccgg caacggttgc cggatgcggc gtaaaacgcc
    19141 ttatccgtcc tggggggctg ggcttggatc ttgtaggcct gataagacgc gccagcgtcg
    19201 catcaggcac tgaatgccgg atgcggcgta aaacgcctta tccggcctac ggttctgtgc
    19261 tcggatcttg taggcctgat aagaggcgtc agcgtcgcat caggcactga atgccggatg
    19321 cggcgtaaaa cgccttatcc gtcctacggc tctgtgctcg gatcttgtag gcctgataag
    19381 acgcgccagc gtcgcatcag gcactgaatg ccggatgcgg cgtgaacgcc ttatccgtcc
    19441 tacggttctg tgctcggatc ttgtaggtct gataagacgc gttagcgtcg catcaggcaa
    19501 aaaacggcaa cgaacacaac aaacacagcc tcacctggca tcaaatttat gaggaaaaaa
    19561 tgaaattact tattctgggc aaccacactt gcggcaatcg tggcgatagc gccatcctgc
    19621 gtggcttact tgatgccatc aatatcctca atccgcatgc cgaagtggac gtgatgagcc
    19681 gctatccggt cagttcttcc tggctgctca accgcccggt aatgggcgat ccgctgttcc
    19741 tgcaaatgaa acaacacaac agcgcggcgg gcgttgtcgg gcacgttaaa aaagtcctcc
    19801 gtcgccgcta ccagcaccag gtattgctct cacgcgtcac cgacactggc aagttgcgta
    19861 atatcgccat cgcccaggga ttcactgact tcgtgcgcct gctgtcaggt tacgacacca
    19921 ttattcaggt cggcggatcg ttttttgtcg atctctatgg cgtgccgcag tttgaacatg
    19981 cgctttgcac gtttatggcg aaaaagccgc tgtttatgat tggtcacagc gtcggcccct
    20041 tccaggatga gcaatttaac caactggcga actatgtctt tggtcactgc gacgcgctga
    20101 tcctgcgcga atcggtcagc cttgatttga tgaaacgcag caatatcacc actgcaaaag
    20161 tggaacatgg cgtcgatacc gcgtggctgg tcgatcacca cattgaagac ttcaccgcca
    20221 gctatgccgt tcaacattgg ctggacgttg ccgcacaaca gaaagcggtg gcaattaccc
    20281 tgcgcgaact ggcaccgttc gacaaacgtc tcggcaccac ccaacaagcg tatgaaaaag
    20341 cctttgccgg ggtggtcaat cgcattctcg atgaagggta tcaggtgatt gcgctctcca
    20401 cctgtacggg catcgacagc tataacaaag acgaccgcat ggtggcgctc aatctgcgcc
    20461 agcacatcag cgatcctgcc cgttaccacg tagtgatgga tgaactcaac gatctggaaa
    20521 tgggcaaaat tctcggtgcc tgtgaactca ccgtcggcac gcgcctgcac tcagccatta
    20581 tctcgatgaa tttctccact ccggcgattg ccatcaacta cgaacataaa tccgccggga
    20641 tcatgcaaca actggggcta ccagaaatgg cgattgatat ccgtcattta ttagacggca
    20701 gcctgcaagc gatggttgcg gataccttag gccagcttcc ggcactgaac acgcgactta
    20761 acgaagccgt cagtcgcgaa cgtcagacgg gaatgcagat ggtgaaatct gtgcttgaac
    20821 gcatcgggga ggcgaaatga aggtcggctt ctttttactg aaatttccgt tgtcgtcgga
    20881 aaccttcgtc ctcaatcaaa ttaccgcgtt tattgatatg ggatttgagg tggagattgt
    20941 cgcgctgcaa aaaggcgaca cacaaaacac ccacgcggca tggacgaaat acaaccttgc
    21001 tgccagaacc cgctggttac aggacgaacc tacgggcaaa gtggcgaaac tgcgccaccg
    21061 cgccagccag accttacgcg gcattcatcg taaaaatacc tggcaggcgc tcaacctcaa
    21121 acgctatggt gctgagtcgc ggaacctgat tttgtctgcc atttgcggcc aggtcgcaac
    21181 accattttat gccgatgtct ttatcgctca ttttggccct gcgggggtaa tcgcagcaaa
    21241 actacgggaa ctgggtatca ttcgcggcaa aattgccacc atcttccacg gtattgatat
    21301 ctccagtcgg gaagtgctca accactacac tcccgaatat caacaactgt ttcgccgtgg
    21361 cgacctgatg ttaccgataa gcgatctgtg ggccggaagg ctgcaaaaaa tgggctgtcc
    21421 gagggaaaaa atcgccgtat cgcgtatggg cgtggacatg acgcgcttta gcccgcgtcc
    21481 ggtgaaagcg cctgcaacac cgctggaaat catttccgtc gcacgcttaa ccgagaaaaa
    21541 aggcctgcat gtggcgattg aagcctgccg tcagttgaaa gagcagggcg tggcatttcg
    21601 ctatcgcatc ctcggcattg gcccgtggga acgacgcctg cgcacgctca tcgaacaata
    21661 tcaactggaa gatgtggtgg agatgccggg ctttaaaccg agccacgaag tgaaagcgat
    21721 cctcgacgac gcggatgtct tcctcctgcc atcggtaacg ggcgcggatg gcgatatgga
    21781 aggtattccg gtggcactaa tggaagcaat ggcggtcggc attccggttg tttcaactct
    21841 gcatagcgga ataccggaac tggtggaggc cgacaaatcc ggctggctgg tgcctgagaa
    21901 cgatgctcgc gcactggcgc aacgactggc ggcgtttagc caactggaca ccgacgaact
    21961 ggctccggtc gtcaaacgtg ctcgcgaaaa agtcgaacac gattttaacc agcaggtgat
    22021 caatcgagaa ctcgccagct tgctgcaggc tttatagagg agaacgtatg ccatttaaaa
    22081 aactctccag acgcaacttc ctgacggcaa gttctgtgct tgccttcctc catacccctt
    22141 tcgctcgtgc gcttcccgcc cgacaaagcg ttaacattaa cgactacaac cctcacgact
    22201 ggatcgcctc atttaaacaa gccttcagcg aagggcaaac ggtcgtcgtg cctgccggat
    22261 tggtttgtga caatatcaac actggcatct tcattccttc tggcaaaacg ttacacatcc
    22321 tcggaagcct gcgcggcaac ggcagagggc gatttgtctt acaggacggc agccaggtga
    22381 caggggagga gggaggcagt atgcataaca tcaccctgga tgtgcgcggt tctgactgca
    22441 ccatcaaagg gctgacgatg accggctttg gcccggtgac gcagatctat atcggcggca
    22501 aaaacaaacg ggtcatgcgc aacctgacca tcgataacct cactgtcagt cacgctaatt
    22561 acgccatctt acgccaggga tttcataatc agattatcgg tgccaacatc accaactgta
    22621 agttcagcga cttacagggg gacgctattg aatggaacgt ggcgattaac gacagcgata
    22681 ttttgatatc tgaccatgtc atcgagcgca ttaactgtac caacggcaaa atcaactggg
    22741 gcatcggcat aggccttgcg ggaagtacct acgataataa ttatccggaa aaccaggcag
    22801 tgaaaaactt tgtcgtggcg aatatcacgg gatcggattg tcggcagttg atccatgttg
    22861 aaaatggtaa acattttgtt attcgtaata tcaaagcccg caatatcacg ccgaatttca
    22921 gcaagaaagc aggcattgat aacgcgacag tcgctattta cggttgtgat aatttcgtga
    22981 ttgataatat tgaaatgatt aatagcgccg ggatgttaat cggctatggg gtaattaaag
    23041 gcaaatatct ctcgataccg caaaatttcc gagtgaataa tattcaactg gataacaccc
    23101 atcttgctta taaattgcgc ggcatccaaa tctccgccgg gaatgctctc tcctttgtgg
    23161 cgctgactaa cattgagatg aagcgtgcgt cgctggagtt acacaacaaa ccgcaacatc
    23221 tttttatgcg aaatatcaag gtgatgcagg aatcctcagt tggaccagca ttgagcatga
    23281 acttcgacat gcgcaaagac gttcgcggcg tctttatggc gaaaaaagaa acactgctgt
    23341 cacttgcaaa tgttcatgcg gtgaatgaaa aagggcaaag ctccgtcgat atcgacagag
    23401 ttaatcacca tattgttaat gtggaaaaga ttaactttag attgccggaa cggagggagt
    23461 agatttgcga ccattcctgg aaaaatggag ccatacttag gaacaatgct actgcaatcc
    23521 acaacgaagc ggcgtaacat cacaagtaat tcagtaatca attcagggta attgatgctg
    23581 gcgaaaaaaa tcgaacaagc tataattcag caaccatttt acaggtggat gaaataatga
    23641 cgaatttaaa agcagttatt cctgtagcgg gtcttgggat gcatatgttg cctgccacta
    23701 aggcgatacc caaagagatg ctaccgatcg tcgacaagcc aatgattcag tacattgttg
    23761 acgagattgt ggctgcaggg atcaaagaaa tcctcctggt aactcacgcg tccaagaacg
    23821 cggtcgaaaa ccacttcgac acctcttatg agttagagtc tctccttgaa cagcgcgtga
    23881 agcgtcaact actggcggaa gtacagtcca tttgcccgcc gggcgtgacc attatgaacg
    23941 tgcgtcaggg cgaaccttta ggtttaggcc actccatttt gtgtgcgcga cctgccattg
    24001 gtgacaaccc atttgtcgtg gtactgccag acgttgtgat cgacgacgcc agcgccgacc
    24061 cgctgcgcta caaccttgct gccatgattg cgcgcttcaa cgaaacgggt cgtagccagg
    24121 tgctggcaaa acgtatgccg ggtgacctct ctgaatactc ggtcatccag accaaagaac
    24181 cactggaccg tgaaggtaaa gtcagcctca ttgttgaatt tatcgaaaaa ccggatcagc
    24241 cgcagacgtt ggactcagac atcatggccg ttggtcgcta tgtgctttct gccgatattt
    24301 ggccggaact tgaacgcacg cagccaggtg catgggggcg tattcagctg actgatgcca
    24361 tcgctgaact ggcgaaaaaa cagtccgttg atgccatgct gatgacaggt gacagctacg
    24421 actgcggtaa aaaaatgggt tatatgcagg catttgtgaa gtatggacta cgcaacctga
    24481 aagaaggggc gaagttccgt aaagggattg agaagctgtt aagcgaataa tgaaaatctg
    24541 accggatgta acggttgata agaaaattat aacggcagtg aagattcgtg gcgaaagtaa
    24601 tttgttgcga attttcctgc cattgtttta tataaacaat cagaataaca acgagttagc
    24661 aataggattt tagtcaaagt tttccaggat tttccttgtt tccagggcgg attggtaaga
    24721 caattagcgt ttgaattttt cgggttaagc gcgagtgggt aacgctcgtc acatcgtaga
    24781 catgcatgca gtgctctggt agctgtaaag ccaggggcgg tagcgtgcat taatacctct
    24841 attaatcaaa ctgagagccg cttatttaac agcatgctct gaagtaatat ggaataaatt
    24901 aagtgaaaat acttgttact ggtggcgcag gatttattgg ttctgctgta gttcgtcaca
    24961 ttataaataa tacgcaggat agtgttgtta atgtcgataa attaacgtac gccggaaacc
    25021 tggaatcgct tgctgatgtt tctgattctg aacgctatgt ttttgaacat gcggatattt
    25081 gcgatgcagc tgcaatggca cggatttttg ctcagcatca gccggatgca gtgatgcacc
    25141 tggctgctga aagccatgtg gatcgttcaa ttacaggccc tgcggcattt attgaaacca
    25201 atattgttgg tacttatgtc cttttggaag cggctcgcaa ttactggtct gctcttgatg
    25261 gcgacaagaa aaatagcttc cgttttcatc atatttctac tgacgaagtc tatggtgatt
    25321 tgcctcatcc tgacgaagta aataataaag aacaattacc cctctttact gagacgacag
    25381 cttacgcgcc aagtagccca tattctgctt cgaaagcatc aagcgatcat ttagtccgcg
    25441 cttggaaacg tacctatggt ttaccgacca ttgtgactaa ttgctctaac aattatggtc
    25501 cttatcattt cccggaaaaa ttgattccat tggttattct gaatgctctg gaaggtaaag
    25561 gattacctat ttatggtaaa ggggatcaaa ttcgcgactg gctgtatgtt gaagatcatg
    25621 cgcgtgcgtt atataccgtc gtaaccgaag gtaaagcggg tgaaacttat aacattggtg
    25681 gacacaacga aaagaaaaac atcgatgtag tgctcactat ttgtgatttg ttggatgaga
    25741 ttgtcccgaa agagaaatct taccgcgagc aaattactta tgtcgccgat cgtccgggac
    25801 acgatcgacg ttatgcaatt gatgctgaga agattggtcg cgaattggga tggaaaccac
    25861 aggaaacgtt tgagagcggg attcggaaga cagtggaatg gtacctgtcc aatacaaaat
    25921 gggttgataa tgtgaaaagt ggtgcctatc aatcgtggat tgaacagaac tatgagggcc
    25981 gccagtaatg aatattctcc ttttcggcaa aacagggcag gtaggttggg aactacagcg
    26041 tgctctggca cctttgggta atttgattgc tcttgatgtt cactccactg attattgtgg
    26101 tgattttagt aatcctgaag gtgtggctga aaccgtcaaa aaaattcgcc ctgatgttat
    26161 tgttaatgct gcggctcata ccgcagtaga taaggctgag tcagaacccg aatttgcaca
    26221 attactcaat gcgaccagcg ttgaatcaat tgcaaaagcg gctaatgaag ttggggcctg
    26281 ggtaattcat tactcaactg actacgtatt tccgggaacc ggtgaaatac catggctgga
    26341 gacggatgca accgcaccgc taaatgttta cggtgaaacc aagttagccg gagaaaaagc
    26401 gttacaggaa cattgcgcga agcatcttat tttccgtacc agctgggtat acgcaggtaa
    26461 aggaaataac tttgccaaaa cgatgttgcg tctggcaaaa gagcgtgaag aattagcggt
    26521 tattaacgat cagtttggtg caccaacggg tgctgagctg ctggctgatt gtacggcaca
    26581 tgctattcgt gttgcactga aaaaaccaga agtcgcaggc ttgtaccatt tggtagccag
    26641 tggtaccaca acctggtacg attacgctgc gctggttttt gaagaggcgc gcaaagcagg
    26701 cattcccctt gcactcaaca agctcaacgc agtaccaaca actgcctatc ctacaccagc
    26761 tcgtcgtcca cataactctc gccttaatac agaaaaattt cagcagaact ttgcgcttgt
    26821 cttgcctgac tggcaggttg gcgtgaaacg aatgctcaac gaattattta cgactacagc
    26881 aatttaatag tttttgcatc ttgttcgtga tggtggagca agatgaatta aaaggaatga
    26941 tgaaatgaaa acgcgtaaag gtattatttt agcgggtggt tctggtacac gtctttatcc
    27001 tgtgactatg gctgtcagta aacagctgtt accgatttat gataaaccga tgatctatta
    27061 cccgctctct acactgatgt tggcgggtat tcgcgatatt ctgattatca gtacgccaca
    27121 ggatactcct cgttttcaac aactgctggg tgacgggagc cagtgggggc taaatcttca
    27181 gtacaaagtg caaccgagtc cagatggtct tgcgcaggca tttatcatcg gtgaagagtt
    27241 tatcggtggt gatgattgtg ctttggttct aggtgataat atcttttacg gtcacgatct
    27301 gccgaagtta atggatgtcg ctgttaacaa agaaagtggt gcaacggtat ttgcctatca
    27361 cgttaatgat cctgaacgct acggtgtcgt tgagtttgat aaaaacggta cggcaataag
    27421 cctggaagaa aaaccgttac aaccaaaaag taattatgcg gtaaccgggc tttatttcta
    27481 tgataacgac gttgtcgaaa tggcgaaaaa tcttaagcct tctgcccgcg gtgaactgga
    27541 aattaccgat attaaccgta tttatatgga acaggggcgt ttatccgttg ccatgatggg
    27601 gcgtggatat gcatggctgg acacgggaac acatcaaagt cttattgaag caagcaactt
    27661 tattgcaaca attgaagaac gtcagggact gaaagtttcc tgtccggaag aaattgctta
    27721 ccgtaaacgg tttattgatg ctgagcaggt gaaagtatta gccgaaccgc tgaagaaaaa
    27781 tgcttatggt cagtatctgc tcaaaatgat taaaggttat taataaaatg aacgtaatta
    27841 aaactgaaat tccagacgta ctgatttttg aaccgaaagt ttttggtgat gagcgtggtt
    27901 tcttttttga gagctttaac cagaaggttt ttgaggaagc tgtaggccgc aaagttgaat
    27961 ttgtccaaga taaccattcg aagtcttgta aaggtgtttt acgcgggctg cattatcagt
    28021 tagaacctta tgcacaaggg aaattggtgc gctgcgttgt tggtgaagtt tttgacgtag
    28081 ctgttgatat tcgtaaatcg tcaccaactt ttggcaaatg ggttggggtg aatttatctg
    28141 cagagaataa gcgccagtta tggatccctg aaggatttgc acatggattt ttggtgctga
    28201 gtgagacggc agagtttttg tataagacga cgaattacta tgcaccaata tatgaaaaaa
    28261 gtattctttg gtctgataat aaaattaata ttgcttggcc tcttcaaggt ggttttattc
    28321 tatccgataa agatcgtaat gcatcgt
//
LOCUS       DAZFJL010000054        28303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFJL010000054 DAZFJL010000000
VERSION     DAZFJL010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.054"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..233)
                     /locus_tag="ACHJ8C_002244"
     CDS             complement(<1..233)
                     /locus_tag="ACHJ8C_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO5552096.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYA"
     gene            complement(304..2418)
                     /gene="fusA"
                     /locus_tag="ACHJ8C_002245"
     CDS             complement(304..2418)
                     /gene="fusA"
                     /locus_tag="ACHJ8C_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO5552097.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2515..2985)
                     /gene="rpsG"
                     /locus_tag="ACHJ8C_002246"
     CDS             complement(2515..2985)
                     /gene="rpsG"
                     /locus_tag="ACHJ8C_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO5552098.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3082..3456)
                     /gene="rpsL"
                     /locus_tag="ACHJ8C_002247"
     CDS             complement(3082..3456)
                     /gene="rpsL"
                     /locus_tag="ACHJ8C_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO5552099.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3582..3869)
                     /gene="tusB"
                     /locus_tag="ACHJ8C_002248"
     CDS             complement(3582..3869)
                     /gene="tusB"
                     /locus_tag="ACHJ8C_002248"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO5552100.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGRISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3877..4236)
                     /gene="tusC"
                     /locus_tag="ACHJ8C_002249"
     CDS             complement(3877..4236)
                     /gene="tusC"
                     /locus_tag="ACHJ8C_002249"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO5552101.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4236..4622)
                     /gene="tusD"
                     /locus_tag="ACHJ8C_002250"
     CDS             complement(4236..4622)
                     /gene="tusD"
                     /locus_tag="ACHJ8C_002250"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO5552102.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRSWQQLNMQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQTGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4622..5344)
                     /gene="yheO"
                     /locus_tag="ACHJ8C_002251"
     CDS             complement(4622..5344)
                     /gene="yheO"
                     /locus_tag="ACHJ8C_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5552103.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5511..6323)
                     /gene="fkpA"
                     /locus_tag="ACHJ8C_002252"
     CDS             complement(5511..6323)
                     /gene="fkpA"
                     /locus_tag="ACHJ8C_002252"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO5552104.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6544..6762
                     /gene="slyX"
                     /locus_tag="ACHJ8C_002253"
     CDS             6544..6762
                     /gene="slyX"
                     /locus_tag="ACHJ8C_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO5552105.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6811..7401)
                     /gene="slyD"
                     /locus_tag="ACHJ8C_002254"
     CDS             complement(6811..7401)
                     /gene="slyD"
                     /locus_tag="ACHJ8C_002254"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO5552106.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7496..7696)
                     /gene="yheV"
                     /locus_tag="ACHJ8C_002255"
     CDS             complement(7496..7696)
                     /gene="yheV"
                     /locus_tag="ACHJ8C_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO5552107.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7706..9511)
                     /gene="kefB"
                     /locus_tag="ACHJ8C_002256"
     CDS             complement(7706..9511)
                     /gene="kefB"
                     /locus_tag="ACHJ8C_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO5552108.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTHFSWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9511..10062)
                     /gene="kefG"
                     /locus_tag="ACHJ8C_002257"
     CDS             complement(9511..10062)
                     /gene="kefG"
                     /locus_tag="ACHJ8C_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO5552109.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGC"
     gene            10193..12106
                     /gene="yheS"
                     /locus_tag="ACHJ8C_002258"
     CDS             10193..12106
                     /gene="yheS"
                     /locus_tag="ACHJ8C_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5552110.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPLQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQNRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12106..13128
                     /gene="yheT"
                     /locus_tag="ACHJ8C_002259"
     CDS             12106..13128
                     /gene="yheT"
                     /locus_tag="ACHJ8C_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO5552111.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPIDAAVIVSAPFMLEACSYHMEKGFSRIYQRYLLNLLKANAARKLAAY
                     PGTLPINLTQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13122..13340
                     /gene="yheU"
                     /locus_tag="ACHJ8C_002260"
     CDS             13122..13340
                     /gene="yheU"
                     /locus_tag="ACHJ8C_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO5552112.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13394..14263
                     /gene="prkB"
                     /locus_tag="ACHJ8C_002261"
     CDS             13394..14263
                     /gene="prkB"
                     /locus_tag="ACHJ8C_002261"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO5552113.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQSLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14318..14722)
                     /gene="yhfA"
                     /locus_tag="ACHJ8C_002262"
     CDS             complement(14318..14722)
                     /gene="yhfA"
                     /locus_tag="ACHJ8C_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO5552114.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15024..15656
                     /gene="crp"
                     /locus_tag="ACHJ8C_002263"
     CDS             15024..15656
                     /gene="crp"
                     /locus_tag="ACHJ8C_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO5552115.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15707..17797
                     /gene="yhfK"
                     /locus_tag="ACHJ8C_002264"
     CDS             15707..17797
                     /gene="yhfK"
                     /locus_tag="ACHJ8C_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO5552116.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQVLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPDKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVTLHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17860..19083)
                     /gene="argD"
                     /locus_tag="ACHJ8C_002265"
                     /gene_synonym="dapC"
     CDS             complement(17860..19083)
                     /gene="argD"
                     /locus_tag="ACHJ8C_002265"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO5552117.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGQARDFLYAGAEEGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAMAKVIGGK"
     gene            complement(19169..19732)
                     /gene="pabA"
                     /locus_tag="ACHJ8C_002266"
     CDS             complement(19169..19732)
                     /gene="pabA"
                     /locus_tag="ACHJ8C_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO5552118.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPECFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19764..20365)
                     /gene="fic"
                     /locus_tag="ACHJ8C_002267"
                     /pseudo
     CDS             complement(19764..20365)
                     /gene="fic"
                     /locus_tag="ACHJ8C_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
     gene            complement(20355..20522)
                     /gene="yhfG"
                     /locus_tag="ACHJ8C_002268"
     CDS             complement(20355..20522)
                     /gene="yhfG"
                     /locus_tag="ACHJ8C_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO5552119.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEALRRHYER"
     gene            complement(20627..21199)
                     /gene="ppiA"
                     /locus_tag="ACHJ8C_002269"
                     /gene_synonym="rotA"
     CDS             complement(20627..21199)
                     /gene="ppiA"
                     /locus_tag="ACHJ8C_002269"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO5552120.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21468..22649
                     /gene="tsgA"
                     /locus_tag="ACHJ8C_002270"
     CDS             21468..22649
                     /gene="tsgA"
                     /locus_tag="ACHJ8C_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO5552121.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22911..25454
                     /gene="nirB"
                     /locus_tag="ACHJ8C_002271"
     CDS             22911..25454
                     /gene="nirB"
                     /locus_tag="ACHJ8C_002271"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO5552122.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25451..25777
                     /gene="nirD"
                     /locus_tag="ACHJ8C_002272"
     CDS             25451..25777
                     /gene="nirD"
                     /locus_tag="ACHJ8C_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO5552123.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYDARVKDG
                     VVQLRG"
     gene            25903..26709
                     /gene="nirC"
                     /locus_tag="ACHJ8C_002273"
     CDS             25903..26709
                     /gene="nirC"
                     /locus_tag="ACHJ8C_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO5552124.1"
                     /translation="MFTDTITKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26728..28101
                     /gene="cysG"
                     /locus_tag="ACHJ8C_002274"
     CDS             26728..28101
                     /gene="cysG"
                     /locus_tag="ACHJ8C_002274"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO5552125.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEYGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6872 a         7561 c         7135 g         6735 t
ORIGIN      
        1 gcgtagtgac gggtcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
       61 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcac gagcagcacc gccgtaggtt
      121 ttagccagta cggtggtgat tgcagcggtc agagtagttt taccgtggtc aacgtggccg
      181 atagtaccaa cgttaacgtg cggttttgta cgttcaaatt tttctttaga cacggctata
      241 ttccttacta tagtgctctc cccttcagga gagagcacgg gactttggta ttaaccctta
      301 ggcttattta ccacgggctt caattacggc ctgagcaacg ttactcggcg cttcatcata
      361 cttcaggaat tccatagtgt atgatgcgcg acctttggtc agagaacgca gctgagttgc
      421 atatccgaac atttcagaca gcggtacttc agcgtggatc ttaacgccag taacttcaga
      481 ttcctgacct ttgagcatac cacgacgacg gctcaagtca ccgataacgt caccggtgtt
      541 ctcttcagga gtttctactt caaccttcat gatcggctca agcagaactg gtttcgcttt
      601 cttaaagcct tctttaaagg cgatagaagc agccagttta aacgccagtt cagaggagtc
      661 aacgtcatgg taagaaccga agtgcagacg aatacccatg tctactaccg ggtagcctgc
      721 cagcggacct gctttcagct gttcctggat acctttatca acggccggga tgtattcgcc
      781 agggattaca ccacctttaa tgtcgttgat gaactcgtag cctttcgggt ttgaacccgg
      841 ctccagcggg tacatgtcga taacaacatg accatactga ccacgaccac cagactgttt
      901 cgcgtgttta ccttcaacat cggtaacttt ctggcggata gtttcacggt aagcaacctg
      961 cggtttacct acgttcgctt caacgttgaa ttcacgcttc atacggtcaa cgatgatgtc
     1021 gaggtgcagt tcgcccatac ccgcgatgat ggtctggtta gattcttcgt cagtccatac
     1081 acggaaagac gggtcttctt tagccagacg gcccagagcc agacccattt tttcctggtc
     1141 agctttggtt ttcggttcaa ctgcgatgga gattaccggc tcagggaatt ccatacgttc
     1201 cagaatgatc ggcgcatccg ggtcacacag ggtgtcacca gtggttacgt ctttcagacc
     1261 gattgcagct gcgatgtcgc ccgcgcgaac ttctttgatc tcttcacgtt tgttagcgtg
     1321 catctgaacg atacgaccga aacgctcacg tgcagctttc acggagttca gtacggtatc
     1381 accagagtta accacaccgg agtacacacg gaagaaggtc aggttaccaa caaacgggtc
     1441 ggtagcgatt ttgaacgcca gtgcagagaa cggctcgtca tcacttgcgt gacgctcagc
     1501 cggagtgtct ttaccgtcgt ccaggatacc gttgatcgca ggtacgtcaa ccggggatgg
     1561 caggtaatca attaccgcat ccagcatcgc ctgaacacct ttgttcttga acgcagaacc
     1621 acaggttacc aggatgattt cgttgttcag aacgcgctga cgcagagcac ctttgatttc
     1681 tgcttcagtc agttcttcac cacccaggta tttttccatc agctcttcag aagcttcagc
     1741 tgcggattcg atcaggttct ggtgccattc gttagccagt tcaaccatgt ctgccgggat
     1801 atcttcgtat tcgaaggtta cgccctggtc agcgtcgttc cagttgatag ctttcatttt
     1861 caccaggtca acaacaccgg tgaaatgttc ttcagcacca atcgccagct gcagcggaac
     1921 cgggttcgcg cccagacggg ttttgatctg gttaacaact ttcaggaagt tcgcacccat
     1981 gcggtccatt ttgttaacga acgcaatacg cggaacttta tatttgtttg cctgacgcca
     2041 tacggtttca gactgcggct gaacaccacc aactgcgcag taaaccatta ccgcaccatc
     2101 gagaacacgc atggaacgtt ctacttcgat tgtgaagtca acgtgccccg gggtgtcgat
     2161 gatgttgatg cgatgcggct catactgctt agccatacca gaccagaatg cagtagtcgc
     2221 agcggaagtg atggtaatac cacgttcctg ctcctgctcc atccagtcca tggttgcagc
     2281 gccgtcatga acttcaccga ttttatggtt tacaccggtg tagaacagaa tacgttcggt
     2341 agtagtggtt ttaccggcgt cgatgtgcgc actgataccg atgttacggt agcgtgcgat
     2401 gggtgttgta cgagccattt gtttcctcgt ttatctttta ggcgttcaat ttaagtagcc
     2461 caaagcgggc tgcttactgg aagcgcccgc ctggtgacta aaactccgaa gggattacca
     2521 acggtagtgt gcgaacgcct tgttggcttc ggccatacgg tgaacgtctt cacgtttctt
     2581 aactgcagta cctttgtttt ctgcagcatc agaaagttcg ttcgccaggc gcagagccat
     2641 ggatttatca ccgcgtttac gagcagcttc aacgatccaa cgcattgcca gagcattacg
     2701 acgaaccgga cggacttcaa ctggtacctg ataagtagaa ccaccaacgc ggcgagactt
     2761 aacttctaca gtcgggcgca cgttttcgag agctacttcg aatgcttcca gttcagattt
     2821 accagagcgc tgagccaggg tctccagcgc gctgtatacg atagattcag cagtagattt
     2881 tttaccatct accatcagga tatttacaaa tttagccagc agttctgatc cgaacttcgg
     2941 atccggcaga attttacgct gaccaatgac gcgacgacgt ggcatggaaa tactccgttg
     3001 ttaattcagg attgtccaaa actctacgag tttagtttga catttaagtt aaaacgtttg
     3061 gccttactta acggagaacc attaagcctt aggacgcttc acgccatact tggaacgagc
     3121 ctgcttacgg tctttaacgc cggagcagtc aagcgcacca cgtacggtgt ggtaacgaac
     3181 acccgggagg tctttaacac gaccaccacg gatcaggatc acggagtgct cctgcaggtt
     3241 gtgaccttca ccaccgatgt aggaagtcac ttcgaaaccg ttagtcagac gaacacggca
     3301 tactttacgc agcgcggagt tcggtttttt aggagtggta gtatatacac gagtacatac
     3361 gccacgtttt tgcgggcatg cttccagcgc aggcacgttg cttttcgcaa ctttgcgagc
     3421 acgtggtttg cgtaccagct ggttaactgt tgccattaaa tagctcctgg ttttagcttt
     3481 tgcttcgtaa acacgtaata aaacgtcctc acacaatatg aggacgccga attttagggc
     3541 gatgccgaaa aggtgtcaag aaatatacaa cgatcccgcc atcaccaggc catctggctg
     3601 gagtgcttaa ccgtaagtct gacgaaatca gtatagtcaa tgggaatgat gtcgttcgaa
     3661 attcgaccag tcaaaccgcg ggcaataagg tcttcgttca gagcatagac cttaatgggg
     3721 gcattacgca gactttcaag gtagcggttg ccgtcaactg cggcagttac gccatcttgc
     3781 aataatagca gttcgtcacc ttcactgagc agacgcagca gcgcagcaaa atccgtcagc
     3841 cagggtgaac gatgtaatgt gtgcagcata aatgcctcaa aacctcaaaa taacatcgta
     3901 gttggcgagt tcgcggcgta aggcatctgc ttcgagcggc gtggcttcga caacaaaagg
     3961 tgtctgcgga tctaacccgc gttcgcgcag tgaagccgca caaacccagc actgttcaat
     4021 atcgtacaga cccaacaatt taaaagtggc aatgtaatca cgcgccagca ctgcatcggg
     4081 cttttgtcct ggcagcagct gaaaaacgcc atcagcaata aagaagacgg ccagatcgtc
     4141 agttaatgcg gaagttgcga gtaaagcatc taatccctcg cggccagccg cggtgccatg
     4201 aggcacagta gaaaaaacaa acgcaattcg tttcatcaga actgtaccac cctgtcacag
     4261 gtcagcgagg cttccgccag cgcgccaagt ccgcttaagg taaatcccgt ctgaaggttt
     4321 gacgaagcca gccccagtct tccggcttcc gtttcatcaa caacgccacg gcgtaatgcc
     4381 gccgctacgc agatattcag cgccacacca tgttgcatat tcaactgttg ccagctgcgc
     4441 acaaggtcaa attcgtcgct tgccggtgag gtcaattggt tagcgttata gaccccttcc
     4501 cgatagaaaa aaacgctgct taactcatgg ccttctgcta ttagcgcctg cgcaaactga
     4561 aaagcactac tcgcctgttg cgtaccgtat gctggcccgg tcaccacgat ggcaaaacgc
     4621 attacttatc ttgcccctgg aaatcaccgc tcttgaactg gcggatgtag agatagacag
     4681 tgtgtttgga gatgttcagg cggtcagcaa cctggttgat cgcgtcttta atatcgaaga
     4741 tccctttctc gtagagattt agcacgatct gacgattttt ggcgttatta gaaacattgc
     4801 gatcggcatt cacttcttcg atggtaaact ccagcgtttg ggtaaccaga tcttcaacag
     4861 aagaggcaaa gttgacgctt gaaccgacat ccggagtttc cggcggcaca aaagtgctca
     4921 taatctgcga gaagggaaca tcaagattca tattgatgca cagcagacca attacacgct
     4981 gttcgcggtt acgaatcgcg atagtcaggg acttcattaa tacgccgctt ttggcgcgag
     5041 taaagtagca tttagaaacg ctgctatccg ctcccgtcat atcgtgcagc atacgtagcg
     5101 ccaggtcagt aattggcgaa ccaatcttcc ggcctgtatg ttcaccgtta gcaatgcgaa
     5161 tggctgaaca ttttagatcc tgcaaagagt gcaaaacgat ttcacagtgg gagccaataa
     5221 gcatcgctaa cccgtccacc accgcttcgt aggatttcag aatatcaaaa tcggtctggt
     5281 caaaaggacg ttgatccagt aaatccaact cactggtttc gttggttaaa agcgacctgg
     5341 acatgaaaaa aaacactcct tttcaggagc ctgtcgttaa cttttcaggg caggctcatt
     5401 aatgatgcgg gtaactaaat taatacagcg gaggttccgc tttccagcac taattatatc
     5461 cggcctgtaa taaaaaaacc gccgcctggt caggcggcgg ttcttagtgc ttatttttta
     5521 gcagagtctg cggctttcgc atcagcttcc ggctttgcat cagccttcgg cgctggtttc
     5581 acatccagca gctctacgtc aaacaccagg gtagaattcg gtgggatccc cggaacaccc
     5641 gctttgccgt aagccagttc tggtggaata accagtttga tcttaccgcc tttcttgatg
     5701 ttcttcagac cttctgtcca acccgggata acaccgtcca gacggaaaga gagcggttca
     5761 ccacgggtgt aagagttgtc gaactcttta ccgtcgatca gcgtaccttt gtagttcact
     5821 acaacagtat cgctgtcttt cggggcttcg cctttaccgg cttctactac ctgataaacc
     5881 agaccagtgg aagaggtttt cacacctttc tctttggcaa atttctcgcg gtactcttta
     5941 ccttttgctt cgttatcagc cgcgtctttt tccatcttcg cctgagcaga agacttcacg
     6001 cgagcttcga atgcttgcag agtctgttcg atctcttggt cggaaagttt gctcttatca
     6061 gcaaatgcat cctgaacacc agcgatcagc tgatctttat ccagtttgat gcccagtttt
     6121 tcttgttctt taagagagtt ttccatgtaa cgacccagcg aagcacccag tgcataagct
     6181 gatttctgat cgtcattttt gaacgctgct ttgctgtcag cagttgtagc aggttttgca
     6241 gcttcagcag caaaagtgat tggtgcatgc agggcaacgg ccattgtggt cgccagcagc
     6301 gttactttaa acagtgattt catccatatc tccaggatcg gggcatctca ccccagggtt
     6361 aactattatc agaagggtac tataaagcgt tgtcgaacaa atctacatac agacacgccc
     6421 tattatcatc tattttcaga ctctttttgt ttaaattagt ttcgatgacc gcgaaatgag
     6481 tgctgtctcg ggcagcaaag ttaagtagaa tccgcggcga ccattcgaca aaagaggtga
     6541 atcatgcagg atttatcatt ggaagcacgc ctggcagagc tggagagccg actggctttt
     6601 caggaaatca ccattgaaga actgaacgtc acggtgaccg ctcatgaaat ggagatggcg
     6661 aaactgcgcg atcatctgcg tctgctgacc gagaagttaa aagccagcca gccgtcgaac
     6721 atcgcgtcgc aggctgaaga aacgcctccg ccacattatt gaggcgtaaa aaaagcgggg
     6781 attccccgct tttttgtcac cttttcgata ttagtggcaa ccgcaaccgc cgttgccttt
     6841 accgccacag cagccttcgc caccgtgttc atgaccgtga tcgtggccat gaccaccgca
     6901 gcaaccgtcg tggtcgtgat cgtggtggtg atcgtgcgcg ccgtgaacgt gaccatgagc
     6961 cagttcttct tcggtcgctt cgcgaatcgc cacaacttca acgttgaatt tcaggttctg
     7021 accagccagc atgtggttac catcaaccac tacgtgatcg tcttcaaccg cagtgatttc
     7081 aaccggtacc ggaccctggt cggtttcagc caggaaacgc atacctacct gcagttcatc
     7141 aacgcccata aatacgtctt taggaacgcg ctgcaccagg ttttcgtcgt actgaccgta
     7201 agcgtcgttc gcgccaacag cgacatcaaa tttgtcgcca acttcatgac cttccagcgc
     7261 tgtttccagg ccagagatca gggaaccgtg accatgcagg tagtccagcg gcgcactcac
     7321 cggagactca tcaaccaaca caccgtcttc tgtacgtacc tgataggcca ggctgaccac
     7381 caggtctttt gctactttca tgatatctcc tgagcatggg aagaatagtg gcgcagattg
     7441 tagcggaatt ctgcagccgt gtactcacta gcttaaaaaa actcggcgca tatcgctagt
     7501 ccggatgaaa aatcccaatc acttgctcat ctttgcggac gtgatcgcgg gcttctttgt
     7561 ctgcttctcg catctgatgt ccacacttaa cacattcaac gatatcaata ttattttcgc
     7621 gccacatcgc cattgaatcc tgtgcctgac aggccgggca ttttgcgccc gcaataaaac
     7681 gttttcggat tgccatcttt accctctact caaattcatc ccagccgtcc agctggcgtc
     7741 gttcttgttg catttcacgc tggaaaatct cttccagttc acgtcgggct tccctggcgc
     7801 gagaaatttg tacggtatca gcatgcatcg ggattaactc ccgcagcatt cgcatatcca
     7861 ggcggcgaaa atgcagttgc gcgcgctgcg cctgatgcgg atgcatgcca agcgtgacca
     7921 gcgtcttgcg ccccagctct aacgcactgg agaatgtttc acgggaaaac tgcgtcaccc
     7981 ctgcctgtaa taactcatgc gcttccacac gtccgcgcgc tcgcgcaaga atatgcaaat
     8041 gcggaaagtg ctgctggcat atttccacca gcttcatagt gtcttccggc tcgttacagg
     8101 taatgacgat agactcagcg gcctctgcac ccgcagaacg taaaagatct acctgcgtgg
     8161 cgtcgccgta ataaactttg tagccatatt tgcgcatcag gttaacggca ctgatatccc
     8221 gctccagtac agtaatgcgc attttatttg ccatcagcaa acgaccaatc acctgaccaa
     8281 aacgcccaaa gcccacgaca atgacctgcg gtttatcatc gttgacccat ggtttttcgt
     8341 cttcttcttc cggtccgtta aactggcggg atagccattt atcgaccagc ttcatcagca
     8401 gcggcgtggt catcatggaa agcgtcaccg tcaccagcaa cagcgccatc tggtcgccct
     8461 ggaataagcg ttgtgaagaa gcggtagaaa agaggacaaa ggcaaactca ccgccctgac
     8521 tcaacacgcc agcaaactgc atccgctcag aactgcgcac gccatacaat cgcgccagca
     8581 gatacagcac gagaattttc accgccacca gcacaaccac acttatcact acccacaaca
     8641 gatgggtata aagcaccccg aggttgagcg acatgccgac agagataaag aacaaaccga
     8701 gcagcaagcc tttgaaggga tcgatagccg tttccagttc atggcgatat tcactttccg
     8761 ccagtagcac acccgcaata aacgtaccga gcgccatcga caaccccagc gcatccataa
     8821 acaatgcgga acccaacacc agcagcagcg tcgcagcggt gaacacttcc cgcacgccag
     8881 aagctgcaat aaaacggaat accggacgca gtaaatagcg cccgccaatc agcatgccga
     8941 caaacgccag caccttcatg ccgatcttca tccagtcgaa atgttcgtct gccgaacccg
     9001 ccaacaacgg cactaacgcc agtgccggga tcaccgccag atcctgaaac agcagtaccg
     9061 agaaacctaa ctggccggat tcgctgcgat tcatcccttt ctcacgcatc aattgcaacg
     9121 ccattgcagt tgaagacatc gcaaggccaa tgccaccgac caccgccgcc tgccatgaga
     9181 aatgggtcag catcaataat cctgccagca acgccgcgct taacagcacc tgtgccgcgc
     9241 ctacgccaaa aatcgaacgc cgcagttgcc aaagcttgga gggattcaac tcaaggccga
     9301 tgataaacat caggaatacc accccgagtt ccgaaaagtg gaggatctca tcgacgtcgc
     9361 taataaaccc cagcccccac gggccaatcg caatccccgc cagcaaatat cccaacactg
     9421 caccaatacc cagccgcgat gccagcggca ccgcagccac cgccgcgaag agaaacagca
     9481 ctcctgcgag taaaaaatca gaaccttcca tcagcagcct cctggagaca gcggatttgc
     9541 cagccagtca ccgtaggctc tggcgtggct cgccagctcc tgtgcgcttt gccgtctcgc
     9601 ccagtaaata atgatggggc ttaaccaatg catccggcac atgcccgccg ccagttcaaa
     9661 ggggcgcaga acatcgctca tcgggtagcg attcaacgcg tcataacggt aagcactttc
     9721 cggctccccg gtggtaatca cgctacgcca gtactttccc gccagttggt ttccccccgg
     9781 tccgctggca aaaccacggc ttaatacccg gtccagccac tctttcagta gcgccgggca
     9841 gctataggta taaagaggat gctgaaagac aatcacctcg tgctcgcgca gtaatgcctg
     9901 ctcacgaggg atatcaataa aaaagtcggg atagtgcgcg taaaggtcgt gcacggtaac
     9961 attgctgagc tgcgtggccg gtttaagcag aacccggttt gccaccgagt cctgagattc
    10021 cggatgggca tacagcagca aaactttcgc tggctgagac atcatccccc tcccggtatg
    10081 gtttttgtgt atagtcgctg ttttgggcta ccattgcgcc cggtgcggca gctcgcccat
    10141 acattacatt atcataatga taagttaaca tagtctgaac atacggcgcc ttatgattgt
    10201 tttttcctcg ttacaaattc gtcgcggcgt gcgcgtcctg ctggataatg ccaccgccac
    10261 catcaaccct gggcagaaag tcggcctggt gggtaaaaac ggctgtggta aatctaccct
    10321 gctggcattg ctgaaaaatg aaatcagcgc cgacggcggc agctacacct ttccgggaag
    10381 ctggcaactg gcgtgggtga atcaggaaac accggcgtta ccgcaagcgg cgctggaata
    10441 tgtcattgac ggcgaccgtg aatatcgtca actggaagcg cagctacacg acgccaacga
    10501 acgtaacgac gggcacgcca ttgcgaccat tcatggcaag ctggatgcta ttgacgcatg
    10561 gagtattcgc tcccgtgccg ccagcctgct gcacggcctc ggtttcagca atgaacaact
    10621 ggagcgcccg gtaagtgatt tctccggtgg ctggcgtatg cgtcttaacc ttgcccaggc
    10681 gctgatttgc cgttcagact tgctgctgct cgacgaaccg actaaccacc tcgatctcga
    10741 tgccgttatc tggctggaaa aatggctgaa gagctatcag ggcacgctga tcctgatctc
    10801 tcacgatcgc gatttcctcg atccgatcgt tgataaaatt attcatatcg aacaacaaag
    10861 catgttcgag tacaccggca actacagttc gtttgaagta cagcgcgcca cccgtctggc
    10921 gcagcaacaa gcgatgtatg aaagccagca ggaacgcgtg gcgcatttgc aaagttatat
    10981 cgaccgtttc cgtgccaaag ccaccaaagc gaagcaggcc cagagccgca tcaagatgct
    11041 cgagcgtatg gagctgattg cccccgcgca cgtcgacaac ccgttccgct ttagcttccg
    11101 cgcgccggaa agcctgccta atccgttatt aaagatggaa aaagtcagcg caggctatgg
    11161 tgatcgcatt attctcgact cgattaaact gaatctggtc cccggctcgc gcattggtct
    11221 gttaggccgc aacggcgcgg gtaaatcgac attaatcaaa ctgttagccg gcgaacttgc
    11281 gccagtcagc ggtgaaattg gtctggcgaa agggatcaag ctcggctact tcgcccagca
    11341 tcaactggaa tacctgcgcg ccgacgaatc gcctcttcaa catctggcac gtttagcgcc
    11401 gcaggagctg gaacaaaaac tgcgtgacta cctcggcggc tttggtttcc agggcgataa
    11461 agtaaccgaa gaaacgcgcc gcttctccgg tggggaaaaa gcccgcctgg tgctggcatt
    11521 aattgtctgg cagcgtccga atctgctgct gctcgacgaa ccgaccaacc accttgacct
    11581 cgacatgcgt caggcactca ccgaagcatt aatcgagttc gaaggcgcgc tggttgtcgt
    11641 ctcgcacgac cgtcatttgc tgcgttccac cactgacgat ctctacctgg ttcacgatcg
    11701 taaagtcgaa ccgttcgacg gcgatctgga agattatcaa cagtggttga gcgacgtaca
    11761 aaagcaggaa aaccagaccg acgaagcgcc aaaagagaac gcgaacagcg cccaggcacg
    11821 taaagatcag aagcgtcggg aagcggagct gcgtgcgcaa acccagccac tgcgtaaaga
    11881 gattgcccgt ctggaaaaag agatggagaa gctgaacgcg caactggcgc aggcggaaga
    11941 gaaactcggc gacagcgaac tgtacgatca aaaccgtaaa gcggagttga ccgcctgcct
    12001 gcaacagcaa gccagcgcca aatccggcct ggaagagtgc gaaatggcat ggctggaagc
    12061 ccaggagcag cttgagcaga tgctgctgga aggccaaagc aactgatggc gcagataaca
    12121 acgaccgatg ccaatgaatt tagcagcagt gctgaattca cccctatgcg cggctttagc
    12181 aattgtcatc tacaaaccat gctgccgcgt ctgttccgtc gcaaggtgaa attcaccccg
    12241 tactggcagc ggctggagtt gcccgacggc gattttgtcg atctcgcgtg gagtgaagat
    12301 cctgcacagg cgcggcataa accgcgttta gtggtatttc acgggctgga aggcagtctc
    12361 aatagccctt acgctcacgg tctggttgag gcggcgcaaa agcgcggctg gctgggcgtg
    12421 gtgatgcatt ttcgcggatg cagcggtgaa ccaaaccgta tgcaccgcat ttaccattcg
    12481 ggcgaaaccg aagacgccag ttggttttta cgctggctgc aacgcgagtt tggtcatgcg
    12541 ccaacggctg ccgtcggcta ttcgctcggc ggtaatatgc tggcctgttt gctggcaaaa
    12601 gaaggcaatg atctcccgat tgatgcggca gtgattgtct ctgcgccgtt tatgctggag
    12661 gcctgtagtt accatatgga aaagggcttt tcccgcattt atcagcgtta cttgctgaac
    12721 ctgttaaaag ccaatgccgc gcgcaagctg gcagcctacc ccggaacact gccgattaat
    12781 ctcacgcagt taaaatcggt acgtcgcatc cgtgaatttg acgatctcat caccgccaga
    12841 attcacggct acgctgacgc tatcgactat tatcgtcagt gtagcgccat gccgatgctc
    12901 aatcggatag ccaaaccgac actgattatt cacgccaaag acgatccgtt tatggatcat
    12961 caggtgatcc cgaaaccgga aagtctcccc ccgcaggtgg agtatcagct gactgaacat
    13021 ggcggtcatg ttggctttat tggcggtaca ttacttcatc cgcaaatgtg gctggagtca
    13081 cgcattcctg actggttaac aacgtatctg gaggcgaaat catgttgatt ccgtggcaag
    13141 acctctcccc cgaaacgctg gaaaatttaa ttgaaagctt tgtgttacgt gaaggcaccg
    13201 attatggtga acatgagcgt acacttgaac agaaagtcgc cgacgtcaaa cgccagctac
    13261 agtgcggaga agcggtgctg gtatggtcgg agctgcacga aacggtcaat atcatgccgc
    13321 gcagtcagtt tcgcgaataa cctgcaaccg tcggcggacg agataataat gatagctaag
    13381 tcatggagtt actatgtctg ccaaacatcc ggtcattgcg gtaacaggat ccagcggcgc
    13441 ggggaccacc accaccagcc tcgcgtttcg taaaatattc gcgcagttaa atctgcatgc
    13501 agccgaggtg gaaggcgaca gttttcaccg ttatacccgc ccggaaatgg acatggcgat
    13561 ccgcaaagcg cgcgacgccg ggcggcatat cagctacttc ggccccgaag cgaacgactt
    13621 cggcctgctg gaacaaacct tcattgaata cggtcagagc ggcaaaggga aatctcgcaa
    13681 atatctgcat acctacgacg aagccgtacc gtggaatcag gtaccgggga cattcacccc
    13741 ctggcaatct ttaccggaac ccactgatgt actgttttat gaaggtttac acggcggcgt
    13801 agtcacgcca cagcataacg ttgcgcagca tgtggactta ctggtcggcg tggtgcctat
    13861 cgttaacctt gagtggattc aaaaactgat ccgcgacacc agcgagcgcg ggcactcacg
    13921 agaagcagtg atggactcgg tagtgcgttc aatggaagac tatatcaact acatcacacc
    13981 gcagttttcc cgcacccatc ttaatttcca gcgcgttccc accgtcgaca cctcaaatcc
    14041 gttcgcggca aaaggtatcc cgtcgctgga tgaaagcttt gtggtgatcc attttcgtaa
    14101 tctggaaggg atcgatttcc cctggctgct ggcgatgttg caaggctcat tcatttccca
    14161 catcaatacg ttagtggtgc ctggcggcaa aatgggtctg gcaatggaat taattatgct
    14221 gccgctggtg caacgattga tggaaggaaa gaaaatcgag taacgctgct attacgccgg
    14281 ataaaatact atccggcttc acaacgggat agttaaatca cgcggcaacc acttcatacg
    14341 agtgagtaat attcaccgct ttttccagca tcagcgccac tgaacaatat ttctcggcag
    14401 agagatcaac cgcacgtgca accgctgcgt ctttcaggtc gcgaccggta acgataaaat
    14461 gcagattaat gtgcgtaaac aggcgtggtg cctcttcgcg gcgttcagag gtcaatttta
    14521 cttcacaatc gaccacatcc tgacgccctt tttgcaggat agaaaccaca tcgatggcac
    14581 tgcaaccacc cgctgccatc aacaccattt ccatcgggct tggtgcttta tcgcctgagt
    14641 tgccgtccat taaaatctgg tgaccagagg cggattcgcc cagaaaagtt aacccttcga
    14701 cccacttcac tcgcgcttgc atttttgcta ctccactgcg tcaattttcc tgacagagta
    14761 cgcgtactaa ccaaatcgcg caacggaagg cgacctgggt catgctgaag cgagacacca
    14821 ggagacacaa agcgaaagct atgctaaaac agtcaggatg ctacagtaat atattgatgt
    14881 actgcatgta tgcaaaggac gccacattac cgtgcagtac agttgatagc cccttcccag
    14941 gtagcgggaa gcatatttcg gcaatccaga gacagcggcg ttatctggct ctggagaaag
    15001 cttataacag aggataaccg cgcatggtgc ttggcaaacc gcaaacagac ccgactctcg
    15061 aatggttctt gtctcattgc cacattcata agtacccatc caagagcacg cttattcacc
    15121 agggtgaaaa agcggaaacg ctgtactaca tcgttaaagg ctctgtggca gtgctgatca
    15181 aagacgaaga gggtaaagaa atgatcctct cctatctgaa tcagggtgat tttattggcg
    15241 aactgggcct gtttgaagag ggccaggaac gtagcgcatg ggtacgtgcg aaaaccgcct
    15301 gtgaagtggc tgaaatttcg tacaaaaaat ttcgccaatt gattcaggta aacccggaca
    15361 ttctgatgcg tctgtctgca cagatggcgc gtcgtctgca agtcacttca gagaaagtgg
    15421 gcaacctggc gttcctcgac gtgacgggcc gcattgcaca gactctgctg aatctggcaa
    15481 aacaaccgga tgctatgact cacccggacg gtatgcaaat caaaattacc cgtcaggaaa
    15541 tcggtcagat tgtcggctgt tctcgtgaaa ccgtgggacg cattctgaag atgctggaag
    15601 atcagaacct gatctccgca cacggtaaaa ccatcgtcgt ttacggcact cgttaatccc
    15661 gtcagagtgg cgcgttacct ggtagcgcgc cattttgttt cccccgatgt ggcgcagact
    15721 gatttatcac cccgatatca actatgcact tcgacaaacg ctggtgctat gtttgcccgt
    15781 ggccgttggg ttaatgcttg gcgaattacg attcggcctg ctcttctccc tcgttcctgc
    15841 ctgttgcaat attgcgggcc ttgatacgcc tcataaacgt tttttcaaac gcttaatcat
    15901 tggtgcgtcg ctgtttgcca cctgtagctt gctgacacag gtgctactgg caaaagatgt
    15961 tcccctgccc tttttgctga ccggattaac gctggtactt ggcgtcactg ctgaactggg
    16021 gccattgcac gcaaaattgc ttcccgcatc gctgctcgct gccattttta ccctcagtct
    16081 ggcgggatac atgccggtct gggaaccgtt gctcatctat gcgctgggca ctttgtggta
    16141 cggattgttt aactggtttt ggttctggat ctggcgcgaa caaccgctac gcgagtcact
    16201 aagtctgctg taccgtgaac tggcagatta ctgtgaagcc aaatacagcc tgcttaccca
    16261 gcacaccgac cctgataaag cgctgccgcc gctgctggtg cgccagcaaa aagcggtcga
    16321 tctaattacc cagtgctatc agcaaatgca tatgctttcc gcgcaaaata ataccgatta
    16381 caagcggatg ctgcgtattt tccaggaggc gctggatttg caggaacata tttcggtcag
    16441 tttgcatcag ccggaagagg tgcaaaagct ggtcgagcgt agccatgcgg aagaagttat
    16501 ccgctggaat gcgcaaaccg tcgccgctcg cctgcgcgtg ctggctgatg acattcttta
    16561 ccatcgcctg ccaacgcgtt ttacgatgga aaagcaaatt ggcgcactgg aaaaaatcgc
    16621 ccgccaacac ccggataatc cggttgggca attctgctac tggcatttca gccgcattgc
    16681 tcgtgtgctg cgcacccaaa aaccgctcta tgcccgtgac ttactggccg ataaacagcg
    16741 gcgaatgcca ttacttccgg cgctgaaaag ttatctgtca ctaaagtctc cagcgctacg
    16801 caatgccgga cgactcagtg tgatgttaag cgttgccagc ctgatgggca ccgcgcttca
    16861 tctgccgaag tcgtactgga tcctgatgac agtattgctg gtgacgcaaa atggctatgg
    16921 cgcaacccgt ctgaggattg taaatcgctc cgtgggaacc gtggtcgggt taatcattgc
    16981 aggcgtgacg ctacacttta aaattcccga aggttacacc ctgaccttga tgctgattac
    17041 caccctcgcc agctacctga tattgcgcaa aaactacggc tgggcgacgg tcggttttac
    17101 tattaccgca gtgtataccc tgcaactgtt gtggctgaac ggtgagcagt acatccttcc
    17161 gcgtcttatc gataccatta ttggttgttt aattgctttc ggcggtactg tctggctgtg
    17221 gccgcagtgg cagagcgggt tattgcgtaa gaacgcccat gacgctttag aagcctatca
    17281 ggacgcgatt cgcttgattc tgagcgagga cccgcaacct acgccactgg cctggcagcg
    17341 aatgcgggta aatcaggcgc ataacactct gtataactca ttgaatcagg cgatgcagga
    17401 accggcgttt aacagccatt atctggcaga tatgaaactg tgggtaacgc acagccagtt
    17461 tattgttgag catattaatg ccatgaccac gctggcgcgg gaacaccggg cattgccacc
    17521 ggaactggca caagagtatt tacagtcttg tgaaatcgcc attcagcgtt gtcagcagcg
    17581 actggagtat gacgaaccgg gtagttctgg cgatgccaat atcatggatg cgccggagat
    17641 gcagccgcac gaaggcgcgg caggtacgct ggagcagcat ttacaacggg ttattggtca
    17701 tctgaacacc atgcacacca tttcgtcgat ggcatggcgg cagcgaccgc atcacgggat
    17761 ttggctgagt cgcaagttgc gggattcgaa ggcgtaatac aggctaaacc atcggatgag
    17821 gtgttcacgc cgcatccgac atcttttgcc tgatgcgtct tatttacccc caatcacctt
    17881 cgccatcgcg tgggcgaaac gatgcatccc ttcatcgata tccgcatctt ccaccaccag
    17941 cgacggcgca aaacgcatca catcaggccc ggcattcagc accattacgc cttcctctgc
    18001 acccgcatac aggaaatcac gcgcctgacc tttgtactgt ggtttcagct ctgcgccaat
    18061 caacagcccc ataccgcgaa tatcgctaaa cacatcgtac tgctgatcga tcttctgcaa
    18121 atggtcaaca aaacgctggc gtttcgcctg aatgccttcc agcacttcag gggtattgat
    18181 gatatcaaac gccgcgcccg ctactgcaca ggccagagga ttaccgccgt aggtggaacc
    18241 gtgagaacca ggatgaaacg cggaagcaat ctccgccgtg gtcagcatgg cgctaatcgg
    18301 gaaaccgcca cctaacgctt tcgcagaggt cagaatatcc ggcgtcacgc cgtagtgcat
    18361 ataggcaaac agatcgccgg tccgccccat cccgcactgc acttcatcaa acaccaataa
    18421 tgcctgatgc tgatcacaca gctcgcgcaa gccctgcaaa aactctggcg tcgctgccgt
    18481 cacgccgccc tcgccctgaa tcggctcaac caccaccgca caggtatggt catccatcac
    18541 ggcttttact gcgtgaagat cgttaaaagg cacgtggatg atgtctgccg gttttggccc
    18601 aaagccgtcg gaatattttg gctgcccacc caccgaaacg gtaaacagcg agcgaccatg
    18661 aaaagcgtta tggaaggcaa taattttggt tttgaacggg ctatgacgca cacaggcgta
    18721 atggcgtgcc agtttaaagg cggtttcgtt agcttccgtg ccggagttca taaacaccac
    18781 gcgttcggca aacgttgcct caatcagttt acgaccaaga cgcagcgccg gttcattggt
    18841 gaaaacgtta ctgatatgcc acagagtttc gccctgggtt tttaacgcgt tcaccagcgc
    18901 aggatggcaa tggcccaacg ccgtaactgc aatgccaccc gcgaaatcga catactcctt
    18961 gccttgctga tcccagattc ggctcccctg accttttacc ggaataaact ctgccggagc
    19021 ataaatcggc agaatcactt catcgaaagt cgcgcgtgta attgctgttt gttcaattgc
    19081 catctcatga tcaccctgtt acgcataaat aaatatgaaa ttataatcac aaaatatgca
    19141 taaaaaatca ctgaatggca atcagaaatc agcgatgcag aaaattagcc agcagttgat
    19201 gtccttgttc gctaagaata ctttctggat ggaattgcac accttccaga tcccactggc
    19261 gatggcgaat ccccataatc tcgcgggttt cgctccaggc cgtcacttca aagcactccg
    19321 gtaatgagtc aggttccacc accagcgaat ggtagcgtgt cacggtgagt ggatttgcca
    19381 gccccttaaa tacgcccaca ccattatgtg taatcggcga ggttttgccg tgcatgacct
    19441 ttgcggcgcg cacaactttg ccgccaaatg cctgcgccat tgcctgatga ccgaggcaga
    19501 cgccaagaat cggcaagcgt ccggcatagt gacgaataac gtcaagggag atcccggctt
    19561 catctggcgt acaggggccg ggtgagatga caattttttg tggtttaagg gcgtcgatat
    19621 ccgccaacgt caacgcatcg ttgcgcttaa ccaacacatc cgcccccagt tcacaaaagt
    19681 actggtagag gttccaggta aaagaatcgt agttatcaat aagcaggatc atggcggctc
    19741 cggtacgaaa gaaccgcgct attttactca gattccccgg cttcgcttac cactttgcta
    19801 aatatcatct gcagcgcggt gagatccccc attgcccatt ctgatttgcc tgattccagg
    19861 cctctttttc gataccctgc cagctcagtt gatatccggc atgaatcgcc agttgctcga
    19921 agaagatccg ctgtgccaaa ccacttccca cccggaaggg atgcagcacg ttgatttcac
    19981 aatagtaatg cgccagccgc tcgacgaact tcgctttctc caggccaacc agataacctt
    20041 cttcctccag atcctgcatc agggcattgc cctctttttc gatataagca aagtggcaga
    20101 acggcgtatc gccctgataa atatcaactt cacgcagttg ccctgcccag tcgaaaatat
    20161 cctgatacaa ctggcgatga agagtaagca aatgcggtaa accccgaacc aacgggccaa
    20221 gttcaatggt cgcagcacgt agcgctgtca tttcgtaagc gacctgttcc agccgctgct
    20281 gctggcggat gttgagctta ttacgcatga tatcgagacc agggtagaga tatgggtcac
    20341 gcccttcgcc gaatttatcg ctcatagtgc ctcctcagcg cttcaaggcg cgctaaagcc
    20401 tctgcggcag taagagtgac taaaggcatc tcgacgcctt caagacggcg actggcctgg
    20461 aaattacgat tacgctgaag ctcccagaga cgggactttt gcttatcggt gagtttcttc
    20521 acttgatgcc tccctgaacg tgtccatttg ccacaagtat aagcggcaaa tccgggttac
    20581 gccgggagag cagaagcgcg ggcaagattg cccgcgcgag aaatcattac ggcaggactt
    20641 tagcggaaag gataactacc ggttttgacg gcacattctg gtacggacca acgtcatgag
    20701 tcggcacctg ggaaatctta tcggcaacgt ccatgccttt caccacttta ccaaataccg
    20761 cgtaaccgaa atcacgctga ccgtggtcaa ggaaggcgtt atcggcaacg ttgataaaga
    20821 actggctggt ggcgctgtct ttgtcagcgg tacgcgccat cgcgatggtg ccacgcgtgt
    20881 tgcgcaggcc gttatctgct tcatttttga ttggcgggtt tggttttttc tgctgcatct
    20941 gctcggtgaa accgccgccc tgaatcataa agccaggaat gacgcggtga aaggtcgtgt
    21001 tgttataaaa gccactgttc acataatcga caaagttttg cacagacact ggcgcttttt
    21061 gtttatccag ctccagttcg atgttaccag ctgaggttgt caacaatacg tgcgggtccc
    21121 ctttcgctgc cattgctgcg ggagaaagag cagaaagagc gaaaacagca gccatcgccg
    21181 ccagggtcga tttgaacatg agatttcctt aacaaacaga gcagaaaaaa agcaagtgaa
    21241 gagattctaa atagccttca agaacaaggc catcccttta cctaatttta cgtatctgaa
    21301 atatatgtaa cctgaggata acttttattc cgtgatcaaa atcacctctt aaatgcaatt
    21361 tagcaaccga ttgcaataaa acatttaatc agatcacaaa atcgcctaaa atcgcccgct
    21421 cgcggcatag cccgctatgc cattttattt ttgaacgcag gccaatcatg actaacagca
    21481 atcgcatcaa gctcacatgg attagctttc tttcctacgc actgaccggt gcgttggtta
    21541 ttgtcaccgg gatggtgatg ggaaatatcg ccgattattt caatctgcct gtttccagca
    21601 tgagtaacac cttcaccttc ctcaatgccg gcattttaat ctctatcttc ctcaacgcct
    21661 ggctgatgga aatcgtcccg ttgaaaacgc agttacgttt tggctttctc ctgatggtgc
    21721 tggcggttgc cggtttgatg ttcagccaca gcctggcgct gttctcggcg gcgatgttca
    21781 ttctcggggt ggtcagcggc atcaccatgt cgattggtac attcctggta acacaaatgt
    21841 atgaaggacg tcagcgcggt tcacgcctgt tatttaccga ctccttcttc agtatggccg
    21901 ggatgatttt cccaatgatc gccgcgtttc tgctggcacg tagcattgag tggtactggg
    21961 tttatgcctg catcgggctg gtgtatgttg ctatttttat tctgaccttc ggctgtgaat
    22021 tcccggcact gggcaaacat gcgccaaaaa cagatgctcc ggtagagaaa gaaaagtggg
    22081 ggatcggcgt actgtttctc tctgttgcgg cgctgtgcta catcctcggt cagttaggtt
    22141 ttatctcctg ggtgccggaa tatgccaaag gcctgggcat gagcctgaat gacgctggca
    22201 cgctggtaag taacttctgg atgtcgtaca tggtcggcat gtgggcgttc agctttattc
    22261 ttcgcttctt tgatttgcaa cgtatcctga ccgtactggc tggtctggct gcgattctga
    22321 tgtacgtctt taacaccgga acaccggcac atatggcgtg gtcaattctc gccctgggct
    22381 tcttctccag cgcgatctat accaccatca tcaccctggg ttcacagcag accaaagtac
    22441 cgtcgccaaa actggttaac tttgtcctga cctgcgggac catcggtact atgttgacct
    22501 ttgtggttac cggcccgatt gttgaacata gtggtccgca ggcggcgctg ctgacggcaa
    22561 acggtctgta cgctgtcgtc tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc
    22621 gtcagcataa caccctgacc tctcattaat tgttcatgcc gggcggcact atcgtcgtcc
    22681 ggccttttcc tctcttactc tgctacgtac atctatttct ataaatccgt tcaatttgtc
    22741 tgttttttgc acaaacatga aatgtcagac aattccgtga cttaagaaaa tttatacaaa
    22801 tcagcaatat accccttaag gagtatataa aggtgaattt gatttacatc aataagcggg
    22861 gttgctgaat cgttaaggta ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag
    22921 tcagactcgc aattatcggt aacggtatgg tcggccatcg ctttatcgaa gatcttcttg
    22981 ataaatctga tgcggccaac tttgatatta ccgttttctg tgaagaaccg cgcatcgctt
    23041 atgaccgcgt acacctctcg tcttacttct ctcaccacac cgccgaagag ctgtcgctgg
    23101 tgcgcgaagg cttctacgag aaacacggca tcaaagttct ggtcggcgaa cgcgctatca
    23161 ccatcaaccg tcaggagaag gtgattcact ccagcgccgg acgtaccgtt ttttatgaca
    23221 agctgatcat ggcgaccggt tcctacccgt ggatcccgcc aattaaaggt tctgatactc
    23281 aggactgctt tgtctatcgc accattgaag acctcaacgc cattgaatcc tgcgctcgtc
    23341 gcagcaagcg cggtgccgtt gttggtggcg gcctgttagg tctggaagct gctggcgcgc
    23401 tgaaaaactt aggcattgaa acccacgtta tcgaatttgc ccctatgctg atggcggaac
    23461 agcttgatca gatgggcggc gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg
    23521 ttcacaccag caaaaacact cttgagattg tgcaggaagg tgttgaagcg cgtaaaacca
    23581 tgcgttttgc cgacggcagc gaactggaag tcgactttat cgtcttctct accggtatcc
    23641 gtccgcgcga taagctggca acccagtgtg gtctggacgt tgctccgcgt gggggtatcg
    23701 tcatcaatga ttcctgccag acttccgatc cggatatcta cgccatcggt gaatgcgcaa
    23761 gctggaacaa ccgtgtattt ggtctggtag caccaggcta caaaatggcg caggtcgccg
    23821 ttgaccatat tcttggtagc gaaaacgcct ttgaaggtgc agaccttagc gccaagctga
    23881 aactgctggg cgtagacgta ggcggtattg gtgatgcgca cggtcgcacg cctggcgcac
    23941 gtagctacgt ttacctcgac gaaagcaaag agatctacaa acgcctgatt gtcagcgaag
    24001 acaacaaaac cctgctcggt gcggtactgg tgggcgatac cagcgactac ggtaacctgc
    24061 tgcaactggt gctgaacgct atcgagctgc cggaaaaccc ggattccctg atcctgccag
    24121 cgcactcggg tagcggtaag ccgtctatcg gtgttgataa actgccggac agcgcgcaaa
    24181 tctgctcctg cttcgacgtc accaaaggcg acctgattgc tgctatcaac aaaggctgcc
    24241 acaccgttgc ggcgctgaaa gccgaaacca aagccggtac gggctgcggc ggttgtattc
    24301 cgctggtcac tcaggtactg aacgcggaac tggcgaaaca gggtatcgaa gttaacaaca
    24361 acctgtgtga acactttgct tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag
    24421 gcattaaaac cttcgaagaa ctgctggcga aacacggcaa aggctacggt tgtgaagttt
    24481 gtaaaccgac cgtcggttcg ctgctggcct cctgctggaa cgaatacatt ctgaagccgg
    24541 aacatactcc gctgcaggat tctaacgaca acttcctcgc gaacatccag aaagacggca
    24601 cctactcggt gatcccgcgt tctccgggcg gtgaaatcac cccggaaggg ctgatggcag
    24661 taggtcgtat cgcgcgtgaa tttaatctct acacaaaaat caccggctct cagcgcctgg
    24721 cgatgtttgg cgcacagaaa gacgatctgc cagagatctg gcgtcaactg attgaagccg
    24781 gcttcgaaac cggtcatgcc tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggaa
    24841 gcacctggtg ccgctacggt gttggcgaca gcgtcggcct cggcgtggaa ctggaaaacc
    24901 gttacaaagg catccgtacg ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg
    24961 aatgttcaga agctcagggt aaagacgtgg gtattatcgc caccgaaaaa ggctggaacc
    25021 tgtacgtttg cggtaacggc ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata
    25081 tcgatcgcga aacgctgatc aaatatcttg accgcttcat gatgttctac atccgtactg
    25141 ccgacaaact gacgcgtacc gcgccgtggt tagaaaacct cgaaggtggc atcgattacc
    25201 tgaaagcggt gatcattgac gacaaactgg ggctgaacgc acatctggaa gaagagatgg
    25261 cgcgcctgcg tgaagcggta gtgtgtgagt ggactgaaac ggtcaatacc ccgtctgcgc
    25321 agactcgctt caaacacttc atcaacagcg ataagcgtga cccgaacgtg cagatggtgc
    25381 cagagcgcga acagcaccgt ccggcaacgc cgtatgaacg tatcccggta actctggtgg
    25441 aggacaacgc atgagccagt ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac
    25501 cggcgtctgc gcgctgttag gtgacgaaca agtagcaatt ttccgcccgt atcacagcga
    25561 tcaggtgttt gcgatcagca acatcgaccc gttcttcgag tccagcgtgc tgtcacgcgg
    25621 gttgattgcg gaacaccagg gcgagctgtg ggtcgccagt ccgctgaaaa agcagcgttt
    25681 tcgcttaagc gacggcttgt gcatggaaga cgaacagttt tccgtcaagc attacgacgc
    25741 gcgagtgaaa gacggcgtgg tacagctgcg cggttaatat tttaacggga ggcgcaatgc
    25801 cttccctttt tgcatggtcc tgtaataacc ttcggtatat tgcaggacat tttttaaact
    25861 ttttgtttta ttttttgttt ttatttttta aaggataatc aaatgtttac agacactatt
    25921 actaagtgtg cggctaacgc tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc
    25981 ttttgggtca gctccgcgat ggcgggcgcg tatgtgggtc ttgggatcat cctgattttc
    26041 acgctcggta atttgctcga cccgtccgta cgtcctctgg tgatgggcgc gacctttggt
    26101 atcgccttaa cgctggtgat tatcgccggt tctgaattgt tcaccggaca caccatgttc
    26161 ctcacctttg gggtgaaagc gggcaccatc agccacgggc aaatgtgggc aatcctgccg
    26221 caaacctggc tgggtaacct ggtcggttcc gtcttcgttg ccatgcttta tagctggggc
    26281 ggcggtagcc tgctgccggt agataccagc atcgttcact ccgttgcgct ggctaaaacc
    26341 actgcaccgg caatggtact gttcttcaaa ggtgcattgt gtaactggct ggtttgcctg
    26401 gcaatctgga tggcgctgcg cactgaaggg gcggcgaaat ttatcgctat ctggtggtgt
    26461 ttgctggcat ttatcgcgtc cggctacgag cactccatcg ctaacatgac gctgttcgca
    26521 ctctcctggt tcggcaacca cagcgaagcc tacacgctgg cgggcattgg tcataacctg
    26581 ctgtgggtga cgctgggtaa tactttatcg ggtgccgtat tcatgggatt gggttattgg
    26641 tatgctacgc cgaaagcgaa tcgtccggtt gcggacaaat ttaatcaaac tgaaacggct
    26701 gccggttaat tactaagggg tttttacgtg gatcatttgc ctatattttg ccaattacgc
    26761 gatcgcgact gtctgattgt cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg
    26821 ttagacgcag gcgctcgctt aacggtgaat gcattagcgt ttattccaca gttcaccgca
    26881 tgggcagatg caggcatgtt aaccctcgtc gaagggccat ttgatgaaag ccttctcgac
    26941 acctgctggc tggcgattgc agcgacagat gatgacgcgc ttaaccagcg cgtcagcgaa
    27001 gccgctgaag ctcgtcgcat cttctgtaac gtggtcgatg cgccgaaagc cgccagcttt
    27061 attatgccgt cgattattga ccgctcaccg ctcatggtag cggtatcctc tggcggcacc
    27121 tctccggttc tggcgcgcct gttgcgcgaa aaactcgaat cactgctgcc gttacatctg
    27181 ggccaggtag cgaaatacgc cgggcaatta cgcgggcgag taaaacagca gttcgccacg
    27241 atgggtgagc gtcgccgttt ctgggagaaa ttgttcgtta acgaccgcct ggcgcagtcg
    27301 ctggccaaca acgatcagaa agccattact gaaaccaccg aacagttaat caatgaaccg
    27361 ctcgaccatc gcggtgaagt ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg
    27421 acactgaaag gtctgcaaca aattcagcag gcagatgtgg tggtctacga ccgtctggtt
    27481 tctgacgata ttatgaatct ggtacgccgc gatgctgatc gcgttttcgt cggcaaacgc
    27541 gcgggatacc actgcgtacc gcaggaagag attaaccaga tcctgctgcg ggaagcgcaa
    27601 aaaggtaaac gcgtggtgcg tctgaaaggc ggcgatccgt ttattttcgg gcgtggcgga
    27661 gaagagctgg aaacactgtg caacgcgggt attccattct cggtggttcc gggtattacc
    27721 gcagcttctg gttgctctgc ctattcgggt attccgctca cgcatcgcga ttatgcccag
    27781 agcgtacgct taattactgg acacttaaaa accgggggcg agctggactg ggaaaacctg
    27841 gcggcagaaa aacagacgct ggtgttttat atggggctga atcaggctgc aactattcag
    27901 caaaagctga ttgaatacgg tatgcctggt gaaatgccgg tggcaattgt cgaaaacgga
    27961 acggcagtca cgcagcgcgt gattgacggt acgctcacgc agctgggcga actggcgcag
    28021 caaatgaaca gtccatcgct aattattatt ggtcgggttg ttggcctgcg cgataaattg
    28081 aactggttct ctaaccatta atttaaccaa ggccagagaa gtctggcctt tttcgtttac
    28141 aactctcttt atatattcag cagataaaaa cataacccct taataaatat tgtctgctaa
    28201 tgggttatat tcaccgttac agcgacttac aaaattaaat caatcgctca ctgacgctaa
    28261 ttgaatattc ctgtatcgtt cttaatgagc agtctgctta tat
//
LOCUS       DAZFJL010000055        28169 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFJL010000055 DAZFJL010000000
VERSION     DAZFJL010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.055"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            97..891
                     /gene="panB"
                     /locus_tag="ACHJ8C_002275"
     CDS             97..891
                     /gene="panB"
                     /locus_tag="ACHJ8C_002275"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO5552126.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            903..1754
                     /gene="panC"
                     /locus_tag="ACHJ8C_002276"
     CDS             903..1754
                     /gene="panC"
                     /locus_tag="ACHJ8C_002276"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO5552127.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(1836..2039)
                     /locus_tag="ACHJ8C_002277"
     CDS             complement(1836..2039)
                     /locus_tag="ACHJ8C_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5552128.1"
                     /translation="MEIKTRRETRQTLAQWFEEKGFQKGYKEGLQKVRLAQRLLSKGM
                     SREDVAEVTALSLAEIDKLINSN"
     gene            complement(2108..3064)
                     /locus_tag="ACHJ8C_002278"
     CDS             complement(2108..3064)
                     /locus_tag="ACHJ8C_002278"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO5552129.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMTFRMMRYAIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNNPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGRQEERQEVRQEVRQEVRQEVRQEV
                     IQEVRQEVRQEYALRFLSKGMSREDVAEMANLPLAEVDKLIN"
     gene            3338..3718
                     /gene="panD"
                     /locus_tag="ACHJ8C_002279"
     CDS             3338..3718
                     /gene="panD"
                     /locus_tag="ACHJ8C_002279"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO5552130.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3722..4951)
                     /gene="yadE"
                     /locus_tag="ACHJ8C_002280"
     CDS             complement(3722..4951)
                     /gene="yadE"
                     /locus_tag="ACHJ8C_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO5552131.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYVFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     DPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5015..5455)
                     /gene="yadI"
                     /locus_tag="ACHJ8C_002281"
     CDS             complement(5015..5455)
                     /gene="yadI"
                     /locus_tag="ACHJ8C_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO5552132.1"
                     /translation="MLGWVITCHDDRAQELLDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHETDSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSAFREHIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5560..6330)
                     /gene="yadH"
                     /locus_tag="ACHJ8C_002282"
     CDS             complement(5560..6330)
                     /gene="yadH"
                     /locus_tag="ACHJ8C_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5552133.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6327..7253)
                     /gene="yadG"
                     /locus_tag="ACHJ8C_002283"
     CDS             complement(6327..7253)
                     /gene="yadG"
                     /locus_tag="ACHJ8C_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5552134.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7362..8024
                     /gene="can"
                     /locus_tag="ACHJ8C_002284"
     CDS             7362..8024
                     /gene="can"
                     /locus_tag="ACHJ8C_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO5552135.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8065..8601)
                     /gene="hpt"
                     /locus_tag="ACHJ8C_002285"
     CDS             complement(8065..8601)
                     /gene="hpt"
                     /locus_tag="ACHJ8C_002285"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO5552136.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8807..11197
                     /gene="gcd"
                     /locus_tag="ACHJ8C_002286"
     CDS             8807..11197
                     /gene="gcd"
                     /locus_tag="ACHJ8C_002286"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO5552137.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEISGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVLRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11393..12943)
                     /gene="cueO"
                     /locus_tag="ACHJ8C_002287"
     CDS             complement(11393..12943)
                     /gene="cueO"
                     /locus_tag="ACHJ8C_002287"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO5552138.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     VIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHSGKFDFHHANKINGQAFDMNKPMFAVAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13109..13456
                     /gene="yacC"
                     /locus_tag="ACHJ8C_002288"
     CDS             13109..13456
                     /gene="yacC"
                     /locus_tag="ACHJ8C_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO5552139.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13562..14428
                     /gene="speE"
                     /locus_tag="ACHJ8C_002289"
     CDS             13562..14428
                     /gene="speE"
                     /locus_tag="ACHJ8C_002289"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO5552140.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTNQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            14444..15238
                     /gene="speD"
                     /locus_tag="ACHJ8C_002290"
     CDS             14444..15238
                     /gene="speD"
                     /locus_tag="ACHJ8C_002290"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO5552141.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTTEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSEDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15276..15638)
                     /gene="yacL"
                     /locus_tag="ACHJ8C_002291"
     CDS             complement(15276..15638)
                     /gene="yacL"
                     /locus_tag="ACHJ8C_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO5552142.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15813..18410)
                     /gene="acnB"
                     /locus_tag="ACHJ8C_002292"
     CDS             complement(15813..18410)
                     /gene="acnB"
                     /locus_tag="ACHJ8C_002292"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO5552143.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFDQLSQYTEKA
                     DGVIFQTAV"
     gene            18765..20522
                     /locus_tag="ACHJ8C_002293"
     CDS             18765..20522
                     /locus_tag="ACHJ8C_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO5552144.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEAQSTQ
                     QTAPDISAVTLPATVSVPPVTPAVVKSTFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSGQSWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKIITTTKKVVPVNQPANAPATQSN
                     TVSTSSPVVAEPAPTVITIEPANPDVVYIPNYNPNVVYGSWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNDWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGMPYHDQDMTKRFHQTDVKGGMSA
                     TPLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGNYDDFRDFS
                     HHQPLTQQQKDAARQRYQSASPEQRQAVREKIQANPQNQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQQRDAARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTARLNNE
                     QRSAVRERLSERGARRLER"
     gene            complement(20764..22188)
                     /gene="lpdA"
                     /locus_tag="ACHJ8C_002294"
     CDS             complement(20764..22188)
                     /gene="lpdA"
                     /locus_tag="ACHJ8C_002294"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO5552145.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22396..24288)
                     /gene="aceF"
                     /locus_tag="ACHJ8C_002295"
     CDS             complement(22396..24288)
                     /gene="aceF"
                     /locus_tag="ACHJ8C_002295"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO5552146.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMIFEVAGEAGAAAPAAKQEAAPAAAPAPAAGMKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENGAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24303..26966)
                     /gene="aceE"
                     /locus_tag="ACHJ8C_002296"
     CDS             complement(24303..26966)
                     /gene="aceE"
                     /locus_tag="ACHJ8C_002296"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO5552147.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27127..27891)
                     /gene="pdhR"
                     /locus_tag="ACHJ8C_002297"
     CDS             complement(27127..27891)
                     /gene="pdhR"
                     /locus_tag="ACHJ8C_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO5552148.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         6733 a         7658 c         6915 g         6863 t
ORIGIN      
        1 gccagacaac ggcggtgcga tacggataaa tcattgcccc caccaatgtg actgataaaa
       61 tgcgagacag cacaacatca tttatcagga tacgctatga aaccaaccac gattgcttca
      121 ttgcagaaat gcaagcagga caaaaaacgt ttcgcaacca tcaccgctta cgactatagc
      181 ttcgcaaaat tgtttgccga agaaggactt aacgtcatgc tggtaggcga ttcactgggc
      241 atgacggttc aggggcatga ctccacgcta ccggttaccg ttgccgatat cgcctatcac
      301 accgccgctg tgcgtcgcgg cgcgccaaac tgccttctgc tggctgacct gccatttatg
      361 gcgtatgcca cgccggaaca agcctttgaa aacgccgcaa cggtaatgcg tgccggagcc
      421 aacatggtca aaattgaagg cggtgagtgg ctggtggaaa ccgtacaaat gctgaccgaa
      481 cgtgccgttc cagtttgtgg tcatttaggc ctaaccccac agtcagtgaa tattttcggt
      541 ggctacaaag tccaggggcg cggcgatgaa gcgggcgatc aactgctcag cgatgcattg
      601 gcattagaag ctgctggggc acagctgctg gtgctggaat gcgtgccggt tgaactggca
      661 aaacgtatta ccgaagcact ggcgatcccg gttattggca ttggcgcagg caacgtcact
      721 gacgggcaga tcctcgtgat gcacgacgcg ttcggcatta ccggcggcca cattcctaaa
      781 ttcgctaaaa atttcctcgc cgaaacgggc gacatccgcg cggctgtgcg gcagtatatg
      841 gctgaagtgg agtccggcgt ttacccgggc gaagaacaca gtttccatta aggagtgacg
      901 ttgtgttaat tatcgaaacc ctgccgctgc tgcgtcagca aattcgccgc ctgcgtatgg
      961 aaggtaagcg cgtggcgctg gtgcccacca tgggtaacct gcacgatggc catatgaagc
     1021 tggtcgacga agccaaagcc cgcgccgatg tggtagtggt cagtattttc gttaacccga
     1081 tgcagtttga ccgcccggaa gatctggcac gttatccacg gaccttgcaa gaggactgcg
     1141 aaaagctaaa caaacgtaaa gtggatttag ttttcgcccc ttcggtaaaa gagatctacc
     1201 cgaacggtac agaaacccac acctatgttg atgttcctgg tctttccacc atgctggaag
     1261 gtgccagccg tccgggacat tttcgcggcg tttcgacgat tgtcagcaag ctgttcaacc
     1321 tggtccagcc ggacatcgcc tgcttcggtg aaaaagattt tcagcaactg gcgctgatcc
     1381 gcaaaatggt tgctgatatg ggctttgata ttgagattgt cggtgtgcca attatgcgcg
     1441 ccaaagacgg tctggcgctg agttcccgta acggttatct gacggcggaa caacgcaaaa
     1501 ttgcgcccgg tctgtacaaa gttttaagtt cgattgccga caaattgcag gctggcgaac
     1561 gggatctcga tgaaattatt actattgccg ggcaagaact gaatgaaaaa ggcttccgcg
     1621 ccgatgatat tcagattcgt gatgccgaca ccttgctgga ggtctctgaa aacagcaaac
     1681 gtgcagtgat tctggtagcc gcctggcttg gcgatgcccg cctgatcgac aacaaaatcg
     1741 tcgaactggc gtaatcctta tctggcgcta cggctgatgg cgccagttat tcattgccac
     1801 gttatctgta ggcaattatt catatcgtag acaacttagt tagagttaat tagcttatca
     1861 atctctgcaa gagataaagc tgtcacctct gcaacgtctt cccgagacat tcctttactc
     1921 agaagacgct gggccagtct taccttctgg agtccctctt tatatccctt ctgaaatccc
     1981 ttctcttcaa accactgcgc caacgtctgc ctggtttctc tccgtgtctt tatttccata
     2041 tgttccctta attatctggt cagttaacaa taacaattat ctggctataa ctgataatgc
     2101 agataactta gttaatcagc ttatcaacct cagcaagagg taaatttgcc atctctgcaa
     2161 cgtcttcccg agacatccct ttactcagaa aacgcagggc gtattcctgt cttacttcct
     2221 gtctaacctc ttgtattacc tcctgtctta cctcctgtct tacctcctgt cttacctcct
     2281 gtcttacctc ctgcctttcc tcctgtcttc ccttctcttc aaaccactgc gccagcgtca
     2341 tcatagactc ccctcccgtt tccctgtctc tcagcacacc ataaaacaaa tccgcttgtt
     2401 cagtatgacc gcgttgcagc atatagtttt gcatggcaac taactgactt ccgctagtgt
     2461 acccttcgtc aatcagcgtg accagttgct ccagcaataa cattaagtcg cgctggcgaa
     2521 tatgtttttg cagtagttcg agaatcgcaa tccgccgatg ttgcatgatt tcgtcatccg
     2581 gtgtgatggt gatatccacc agcgggaaag ggttgttgta aacgcgtcgc gccagctccg
     2641 gcgagtaaaa catatcaaac cagcacattg agagcggata aggtgtggcc tcgccctgat
     2701 aaaacagaat cggcaccacc agcggcagct tatcgtgatc cgcctccaga tgccggtgca
     2761 tggcggcaat ggcatagcgc atcatgcgaa aggtcatttt cttatccggc ttgctttgat
     2821 gttcaatcac cacatgcaaa taaccgggat taccctgcat ttgcacggaa taaagcacgt
     2881 cagtgctgtg ccctttcagg ctttcttcaa taaaactccc cgactcaaga tgcagcgtgt
     2941 cgaggtcaca cagttcgcgt agttccgctg gcaaatggat atccagaaag tcacgagccg
     3001 tttccgcatg cattaaaaat tgtttaaata ccgcgtcatg cggtgtggta ctcggtgcat
     3061 ccatcttccc tgccttccgt gttgtcttgc gtgaaggcag tatccagtca aggcgcgccg
     3121 tcgtcagccg cctctttatt gtttaccgag cagcgttcag agaaatttca attggttaca
     3181 ggcgttacat ggcacgcttc ggcacggtac gcaaactgct gatgcaaatg ccgtcagatg
     3241 agtagacact aaacaaaaat cgggcaatac tgcgtgagaa tttcctcaaa gcaggcatcg
     3301 cctgcttcgt taacgacagg gtagaaaggt agaagttatg attcgcacga tgctgcaggg
     3361 caaactccac cgcgtgaaag tgactcatgc ggacctgcac tatgaaggtt cttgcgccat
     3421 tgatcaggat tttcttgacg cagccggtat tctcgaaaac gaagccattg atatctggaa
     3481 tgtcaccaac ggcaagcgtt tctccaccta tgccatcgcg gcagaacgcg gttcgagaat
     3541 tatttctgtt aacggtgcgg cggcccactg cgccagcgtc ggcgatattg tcatcatcgc
     3601 cagcttcgtt accatgccag atgaagaagc tcgcacctgg cgacccaacg tcgcctattt
     3661 tgaaggcgac aacgaaatga aacgtaccgc gaaagcgatt ccggtacagg ttgcctgatt
     3721 gttatccctg cggctggtta ctcaccagcc gcgacatcgt ctccagcgaa tccgttctta
     3781 agatataaag tcgttttagt aacaacggat tatcccccgg ttttactttg cctttcatgg
     3841 ttgtcaccgc caggtgaaat ccggcatcgt ttgctgcctt cacggctttg tcattaaatc
     3901 cgccaaacgg atacgaaaga taccagacat gcggattaaa ttgcgccaga gcgcggcgtg
     3961 aacgtgcaaa atcaaacaga atattgtgct cactacggct cagtaatatg ggtcggcgat
     4021 aaccatccac ccgatgcaaa aaatgggtat gtgactggaa atcaaataca tcgcgaattt
     4081 cgttaagttc agaaacgctc ataaattgca gcgattttgg gtcccacttc tgcgggtgac
     4141 gtttgatgcg cgaggtaaca ataaacgccg tcgccttcat accatattgt ttcaacacag
     4201 gatacgcata gcggctcacc gacttgaggc catcatcaaa ggtaattacc accgctcgcg
     4261 cagggagatt gatcttattc ttcacgtaac cttccagctg cgccatgctc agtgtcgcgt
     4321 atcccctgtc gcgcagccag gccatctggt tattgaaagc gcgtaccgag gtggtcgtcg
     4381 aagtatggcg aaaacgggtg ttttcttcgt cgcgcaaaat atggtgatag gttagcactg
     4441 gcaggccatt atcgggttgg gcatccagcg cgctgatata ggccagtcga tcgccaatac
     4501 ggatctgata ccaggtttga tttaacctgt ctttcagttt atgcaaaatc gggtagcgca
     4561 aattgtccgc cagtactcca aatggcgcac tgcccacact cggcgcgtta taaaccggcg
     4621 tatctttcca ggttattaag ttctgattac tcagcggctt gttgaggtca cccaaaccat
     4681 cttcaacttt ttgtcgcccc tgaaccggct cgagatggcc tttatcgata aaccctttac
     4741 caaagccaaa attaaatacg taataacttg cggcagtggg ctccacggca atgatttgtc
     4801 cggcccgaat attccctacc gtcaccattt tgtcgccaat ttgcgcccag accgcggcat
     4861 tttcgatggt ttgcatataa cgggcaggta acgcggcact gacacttgcg gtaaacagca
     4921 tcagcaggag aataacagct tgtttgtaca tgaaaaaata accgagggtg aaacaacgct
     4981 tttattgtag caaaagcgtc atcagaatcg cgggttaata ctcatacaaa ttatgtttga
     5041 ggacgaaagg cggatttttt tgttgttggt gccaaagact actcacctcc ggcgcaccca
     5101 gttcgacaat atgctcgcga aacgctgaac tggtcatggt ttcacggcaa gccatcatct
     5161 gttcaattaa cggtaacgtg acaccagaaa tcacttcgca acgggagtgt ttgtggctta
     5221 ataatgaagc cacgcggtac ggtggcgctc cggctatatc ggttaaaaag ataacgccct
     5281 caccagagtc cgtttcatgc aaggcatcgc acatcatgcg gctgagcata ttagagctta
     5341 atccgcgcca gaaattcacg gcccggcact gaagaagtgc cccatgtttt ttctccagcg
     5401 catccagtag ctcttgcgcc cggtcatcgt gacaggtaat tacccaacct aacatcgcct
     5461 tcctccttag ccgaaaatca gtctaccctg accattgttg ataaagggtg atagctgtca
     5521 aaactcctct cccccaaatc cagaggagag gaaatagcct tagctacgca aaccacgtcc
     5581 acgttggatc agcgaccaac agatcaaata aaacgccaca ataaagacca ccagtacgcc
     5641 aaaggtagtg accagcggaa catcattgat accgaggaag ccgtagcgga aaccactgat
     5701 catataaacg attgggttca ggtgcgacag cccttgccag aacggcggca acaaagtcag
     5761 ggagtaaaac accccgccca aatacgtgag tggcgttaac acaaaggttg gcaccaggct
     5821 gatgtcatcg aacgttttgg caaacacgcc gttcagcaaa cccgcaaggg agaatagcac
     5881 cgcagtgagc accagggtta aggcaacgaa tacccacgaa tgcacctgaa acggcacaaa
     5941 aaacagtgaa attgccgtca ccaaaatacc aacaaacaga ccacgcgcca cgccaccacc
     6001 gacatatccg gcaataatga catgagtcgg aaccggcgct accagcagct cttcaatatt
     6061 acgctggaac ttggcaccaa aaaatgatga cgcgacgtta gcgtaggcgt tggtgatcac
     6121 cgacatcatg atcagccccg gtacgatgaa ctgcatgtag ctgaagccat gcatatcgcc
     6181 aatacgcgaa ccaatcaggt taccgaagat aataaagtaa agggtcatgg tgatgactgg
     6241 cggcaccagc gtctgcaccc agatacgcat aaagcgatgg atctctttcg cccagatgct
     6301 ttttagtgcc acccagtaaa gatgcatcat gcgcgatctc cttgtttttc attaaccagt
     6361 gaaacaaaca gctcttccag acggttagct ttgttacgca tacttaatac ctgaatgccc
     6421 tgctcactta actgcgtaaa tacgctgttg atcccctgct cacgcagcac ttcaacttcc
     6481 agcgtcgcgg tatcgaccag tcgatactga tagccatcga gcttcggtaa cgggcttttc
     6541 ggtgcgagat cgagaataaa ggtttccgat ttcagcttcg ccagcagcgc cttcatcgag
     6601 gtattttcca ccagctcacc gtgttgaata atgccgatat tgcggcacag catttctgct
     6661 tcttccaggt agtgtgtggt gagaatgata gtggtgcctt tgtcgtttaa atccttcaaa
     6721 aagccccaca ttgagcggcg aagttcgata tccacgcctg cggtcggttc gtcgagaatc
     6781 agtagtttag gttcatgcat taacgcacgg gcaatcatta aacggcgctt catcccgcca
     6841 gataacatgc gcgcacgttc gttgcgtttc ccccatagat cgagctgttt aagatacttt
     6901 tcgctgcgga tgtacgcttc tttgcgctcc acaccgtagt accctgcctg attcaccaca
     6961 atttgctgta cggtttcaaa cgggttgaag ttaaattcct gcggcaccag tcccaactga
     7021 cgtttagcgt tcacgacatc cttctcgaga tcgtaaccaa atacgctgac ccgcccggag
     7081 gttttattta ccagagagct gataataccg atagtggtcg atttcccggc cccgttcggc
     7141 ccgagaagcg cataaaaatc acccgcttcg acctgcaaat cgattccacg aagcgcctga
     7201 acgccgcctg gataggtttt tttaagctgt tgaagttcca gtgcaatggt cataaatttt
     7261 tacttacctt acgttcttac actttatctg tggtttaaat cgtcccggag ttgccctata
     7321 ttagccaaac gtaattattt ggttacaggt cgttaacctc catgaaagac atagatacac
     7381 tcatcagcaa caatgcacta tggtcaaaaa tgctggtgga agaggatccc gggttttttg
     7441 agaaactggc acaagcgcaa aaaccgcgct ttctatggat tggatgttcc gacagtcgcg
     7501 ttcctgcaga acgtttaacc ggtcttgagc cgggcgaact ctttgttcac cgtaatgttg
     7561 ctaacctggt cattcacacc gacctgaact gcctttccgt ggttcagtat gcagtggatg
     7621 tactcgaagt tgaacacatt attatctgtg gccactacgg ttgcggcggc gtacaagccg
     7681 cagttgaaaa cccggaactg gggcttatca acaactggct gctgcatatc cgcgatatct
     7741 ggttcaaaca tagctcattg ctcggcgaaa tgccgcaaga gcgccgtctg gataccttgt
     7801 gtgaactgaa cgtcatggaa caggtgtata acctgggcca ctccaccatt atgcaatcag
     7861 cgtggaaacg cgggcagaaa gttaccattc acggctgggc ctacggcatt cacgacggct
     7921 tgctgcgtga tctggatgtc acagcgacta accgcgaaac ccttgagcaa cgttaccgtc
     7981 acgggatctc caacctcaag ctgaaacacg ccaaccacaa ataaaaatgc catgccggat
     8041 gcaacacatc cggcaacttc acacttactc gtccagcaga atcactttgc cgatatacgg
     8101 cagatgacgg taacgctgtg cgtaatcaat gccgtaaccc accacaaact catccgggat
     8161 cgagaaaccg ataaattcta ccgggacgtt cacttcacga cgggacggtt tatccagcag
     8221 cgtacaaatc gccaacgact tcggttcgcg caggcttaag atctcacgca ctttcgacag
     8281 tgtattcccc gagtcgatga tatcttcaac aatcagcacg tccttgccac ggatatcttc
     8341 atccagatct ttgaggattt tcacatcacg ggtggtggac atgccgctac cgtagctgga
     8401 ggcggtcata aagtcgactt catgagatac ctgaacttca cggcacaggt ccgccataaa
     8461 cataaatgag ccacgcagca gacccaccag caccatatcg ctgccgctgt ctttgtaacg
     8521 ctcagtaatc tgacgaccca gttcggcgat acgcgcttta atctccgctt cggggatcat
     8581 tacttctaca gtatgtttca tatctataac catatgattt aaaaacaaat cacttcgcac
     8641 gggcaggttt tggcaccgca acaaagcgca agacacgcag tataccagta aaactattca
     8701 ttcgcggaat ctgttaataa agctcgaatt gtgatgacga tcacacatgt taaaacctat
     8761 aattaattgc tactaaaata ttaatgaatt gaaatggtgt ctctttatgg caattaacaa
     8821 taccggctcg cggcgattac tcgtcacgct aacagccctt tttgcagcgc tttgcgggct
     8881 gtatctactc attggcggag gctggctggt cgcgattggc ggctcctggt actaccctat
     8941 cgctggcctt gtgatgctcg gcgtcgcctg gatgctgtgg cgcagtaaac gcgccgcgct
     9001 ttggctgtac gcagccctgc tgctcggcac tatgatttgg ggcgtctggg aagttggttt
     9061 cgacttctgg gcgctgactc cgcgcagcga cattctggtc ttcttcggca tctggctgat
     9121 cctgccgttt gtctggcgtc gcctggtcat tcctgccagc ggcgcagttg ccgcactggt
     9181 ggtggcgctg ttgattagcg gtggtatcct gacctgggcc ggatttaacg atccgcagga
     9241 aatcagcggc accttaagcg ccgatgccac acctgccgaa gccatctccc ccgttgccga
     9301 tcaggactgg cctgcctatg gtcgcaatca ggaaggccaa cgcttttcac ctctgaaaca
     9361 aattaacgct gataacgtcc acaagctgaa agaggcctgg gtgttccgta ctggcgatgt
     9421 gaagcagccg aacgatccgg gcgaaatcac caatgaagtg acgccaatta aagtgggcga
     9481 caccctttac ctgtgtaccg cgcatcaacg tctgtttgcg ctggatgccg ccagcggtaa
     9541 agagaaatgg cattacgatc ctgagctgaa aaccaacgag tctttccagc acgtaacctg
     9601 ccgtggcgtc tcttatcatg aagccaaagc agaaactgct tcgccggaag tgatggcgga
     9661 ttgcccgcgt cgtatcattc ttccggtcaa tgatggtcgt ctgattgcaa ttaacgctga
     9721 aaacggcaag ctgtgcgaaa ccttcgccaa taaaggcgtg ctcaatctgc aaagcaatat
     9781 gccggacacc aaaccgggtc tgtatgaacc gacttcgccg ccgattatca ccgataaaac
     9841 catcgtgatg gccggttcag tcaccgacaa cttctcaacc cgcgaaacgt ctggcgtgct
     9901 ccgtggtttt gatgttaaca ccggggagct gctgtgggct tttgatccgg gcgcgaaaga
     9961 cccgaatgcg atcccgtctg acgaacacac ctttaccttt aactcaccaa actcgtgggc
    10021 accagcggcc tatgacgcga agctggatct ggtctatctg ccgatgggcg tgaccacgcc
    10081 ggatatctgg ggcggtaacc gcacaccgga acaggaacgt tatgccagct cgattctggc
    10141 gctgaatgcc actaccggga aactggcgtg gagctaccag accgttcacc acgacctgtg
    10201 ggatatggat cttccggcac agccgacgct ggcggacatc accgttaatg gtcagaaagt
    10261 gccggtcatt tatgctccgg cgaaaaccgg caacattttt gtgctcgatc gtcgtaatgg
    10321 cgaactggtg gttccggcac cggaaaaacc ggttccccaa ggcgcagcga aaggtgatta
    10381 cgtgacccct actcaaccgt tttctgaact gagcttccgt ccgacgaaag atttgagcgg
    10441 tgcggatatg tggggagcca ccatgtttga ccagctggtg tgccgcgtga tgttccacca
    10501 gatgcgctat gaaggcattt tcaccccgcc atctgaacag ggtacgctgg tcttcccggg
    10561 taacctgggg atgttcgaat ggggcgggat ttccgttgat ccgaatcgtg aagtggcgat
    10621 tgccaaccca atggcactgc cgtttgtttc gaaactgatc ccacgcggtc caggcaaccc
    10681 gatggaacag ccgaaagatg ccaaaggcac gggtacggaa tccggcatcc agccgcagta
    10741 cggtgtaccg tatggtgtca cgctcaaccc gttcctctca ccgtttggtc tgccatgtaa
    10801 acaaccagca tggggctata tctcagcatt ggatctgaaa accaatgaag tggtgtggaa
    10861 gaaacgtatt ggtacgccac aggacagtat gccattcccg atgcctgtac cggtgccgtt
    10921 caatatgggt atgccgatgc ttggcgggcc aatctccacg gcgggtaacg tgctgtttat
    10981 cgccgctacg gcagataact acctgcgcgc ttacaacatg agcaacggtg aaaaactgtg
    11041 gcagggtcgt ttaccagcgg gtggtcaggc tacgccaatg acctatgaag tgaatggcaa
    11101 gcagtatgtg gtgatctccg caggcggtca cggttcattt ggtacgaaga tgggcgacta
    11161 tattgtggct tatgcgctgc cggatgatgt gaagtaaggc ttgctcagat tgctgacaac
    11221 gtgcgatttg ttcatgccgg atgcggcgtg aacgccttat ccggcctaca aaacatttgc
    11281 aaattcaata cattgcgcgt aggcctgata agcgtagcgc atcaggcagt tttgcgtttg
    11341 tcatcagttt catgcccgga gagatccggg catatttccg aatacggtct ttttataccg
    11401 taaaccctaa catcatcccc gtatcttcat gctccagcag atggcagtgc gccatataag
    11461 catgttcttt cggtgcatcg tgattaaact tcaccagcac ttcgctgaca ttaccttcta
    11521 ccttaacggt atctttccag cctgcgcgat gcgtcgctgg cggtttgcca ttttctgaca
    11581 agatacggaa ctgcgtaccg tggatatgga acggatgcag catcatgtcg cccacgccag
    11641 agataaccca acgttcgtac tgccctttcg ccaccgcaaa catcggctta ttcatatcaa
    11701 acgcctgacc gttgattttg ttggcatggt ggaaatcaaa cttcccgctg tggttcatat
    11761 gattcatatt gccgtgcccc atatggccca tcatctggct gtgatccatc ccggccatcg
    11821 cctgatcgcc atatttctcc atcagcatct gcatccccat catatcgagc attgggtcca
    11881 tagaaagttg cagcttgcgt accgtcagcc cttccagcga aggtaacgca ggcaggctac
    11941 ttaatgtgtc tggcaaagca ccagaggcac taatggcaat cggctgaatc cgcattaccg
    12001 gatgcggctt gtcaaacggc gcaatcgcca tccccatctg gctgaccggc agcgtcacca
    12061 ggtcaaaggg tttgttgtcg ttaacctcca ccagcacttc aaaacgctcg cccatcagca
    12121 ctggcagctc gttcaccttc accggttcag gtagcaggcc gccgtcgctg gcaatcacat
    12181 aaagcgggcg attgtcgctg gtggcgaaat tgagcgagcg ggcattacag ccattgagca
    12241 aacgcaggcg cagccagcca cgcggggcag cgtgttgcgg gtagattaca ccgttggtca
    12301 gcaacgtatc gccaaaccaa cccacagcgg cggtcatcac atccagttga taatcaatct
    12361 gcccgtcagc gttaaatttt ttatcctgaa caatcaccgg gacatcatca ataccccact
    12421 gttttggcag cattaatttc aggatctcgt catcttcaat caccaccaaa ccagccagcc
    12481 ccatcgccac ctgtcgcccg gtcttgccat gttgatgtgg atggaaccaa caggtagcgg
    12541 caggttgatc aacgttcaac gtcaccgagc gcttgccacc tggcggaata attccctgcg
    12601 ggccgccgtc cacttcaccc ggtacttcca gcccgtgcca gtgcaacgtc gtctcttccg
    12661 tcagttggtt gtagatatca accgttaccg ctttgccgcg ctgtaatttc accgccggcc
    12721 ccagcagatt gccgttatag ccccaggtag ttgcggtttt cccgccaaag gtggactgac
    12781 ctgcgccaat agttaactga atgcgattac gggcatcggt cgtgagcaaa tcagggattg
    12841 gtaacgttgg gcgttccgcc gcaaatactg cgcggctcca cagcggcaag gctgaagcca
    12901 cacccagcgc gacggaatat ttcaagaaat cacgacgttg catagttatt tccttattct
    12961 taagcaggcg aaacaaaatc aaacgttgag catagtcctt ccccttacgg gaaggtcaag
    13021 ccgcagacac aatttaatcg acaataatgg tcgtcgcctc gctcgcagtg tgctaacgtt
    13081 tatcttcttt aagcccctgg tagaagcaat gaagacgttt ttcagaacag tgttattcgg
    13141 cagcctgatg gccgtttgcg caaacagtta cgcgctcagc gagtctgaag ccgaagatat
    13201 ggccgattta acggcagttt ttgtttttct gaagaacgat tgtggttacc agaacttacc
    13261 taacgggcaa attcgtcgcg cgctggtctt tttcgcccag caaaaccagt gggatctcag
    13321 taattacgac accttcgaca tgaaagccct cggtgaagac agctaccgcg atctcagcgg
    13381 cattggcatt cccgtcgcta aaaagtgcaa agccctggct cgcgattcct taagcctgct
    13441 tgcctacgtc aaataatccc tgataccttt ttgtagaaat attgaccgtg catccgcggt
    13501 caatgttagc tattatgttg cgccattttt ttacgggtgt taacaaagga ggtatcaacc
    13561 catggccgaa aaaaaacagt ggcatgaaac gctacacgac cagtttgggc agtactttgc
    13621 ggtagataac gttctgtatc atgaaaagac cgatcaccag gatctgatca tttttgagaa
    13681 cgccgcattt ggtcgcgtaa tggcgctgga tggtgtagta caaaccaccg agcgtgacga
    13741 gtttatctat catgagatga tgacccatgt tccgctactg gcccacggtc acgcgaaaca
    13801 tgtgctgatt atcggcggcg gcgacggtgc catgctgcgt gaagtaaccc gacataaaaa
    13861 cgttgagtca atcacgatgg tggaaatcga tgcgggtgtc gtgtcgttct gccgtcagta
    13921 tcttcccaac cataacgccg gtagttacga cgatccgcgc tttaaactgg tgatcgacga
    13981 tggtgtcaat ttcgttaatc aaaccaacca gacctttgat gtcattatct ccgactgcac
    14041 cgatcctatc ggtcccggcg aaagtctgtt tacttctgca ttttatgaag gctgcaaacg
    14101 ttgcctgaat cctggcggta tcttcgtcgc acaaaacggc gtctgctttt tacagcagga
    14161 agaggccatc gacagtcatc gcaaactcag ccattacttc agcgatgttg gcttttatca
    14221 ggcggcgatc ccgacctatt acggcggcat aatgaccttt gcatgggcga cagataacga
    14281 cgccttacgc catctctcaa ccgaaattat tcaggcgcgt tttctcgctt ctggcctgaa
    14341 atgccgttat tacaatccgg cagtccatac ggcagctttt gccttacccc agtatctgca
    14401 agatgcactg gcttcacagc cgtcctaagg agaagataag aaattgaaaa aactgaaact
    14461 gcatggcttt aataatctga ccaaaagtct gagtttttgt atttacgata tctgctacgc
    14521 caaaactacc gaagagcgcg acggttatat tgcttatatc gatgaactct ataatgccaa
    14581 ccgtctgacc gaaatcctgt cagaaacctg ttccattatc ggggccaata ttcttaacat
    14641 cgcccgccag gattacgagc cacagggtgc cagcgtcact attctggtga gcgaagaacc
    14701 ggtcgacccg aaacttatcg acaaaacaga acaccccggc ccactgccag aaacggtcgt
    14761 tgcccatctc gataaaagtc atatttgcgt acatacctac ccggaaagtc atcctgaagg
    14821 cggtttatgt accttccgcg ccgatattga agtctctacc tgcggcgtga tttctccgct
    14881 gaaggcgctg aattacctga tccaccagct tgagtccgat attgtgacta ttgattatcg
    14941 cgtgcgcggt tttacccgcg acattaacgg tatgaagcac tttatcgacc atgagattaa
    15001 ttcgattcag aactttatgt ccgaagatat gaaggcgctg tatgacatgg tggatgtaaa
    15061 cgtctatcag gagaatatct tccataccaa gatgttgctt aaagagttcg accttaagca
    15121 ctacatgttc cacaccaaac cggaagattt aaccgacagc gagcgccagg aaattaccgc
    15181 tgcgctgtgg aaagaaatgc gcgagattta ttacgggcgc aatatgcccg ctgtttaacg
    15241 tctcaggcgg agcacggagg ctccgcctga tttacttact tctgctgcac gaaattacgg
    15301 taagccgcca ccacctgaag aaaatcctca acgccgcaca gcgacaagct ctcttcatcg
    15361 tagtagttca tcccctcttc catttcatcg ccagcgaatt ccagttgatt ggcgcgaacc
    15421 atcacctctt caccatccat ccatagcgtg tattcatgcc ctgcccgttg ccaggaacgt
    15481 tcgctacctt tcaatgcgtg cgccgcttgt tctacttcat caagcaaggc caggttttct
    15541 ttcacctctt cattaaacca gtgcccgacc acttcatgcc ccatggacat gcgcaccttt
    15601 accactccgg taatatcgcg cagaaattcg taatccatag tgtgttcctc tgctcctggc
    15661 aatgcgccga aatcgcattg cgccattgat gtaattatcg cagccttacc ggagaaaaac
    15721 agcggaacgg cgatggttta gaaataaaaa atgcccggga atctcattca ccgggcattg
    15781 tgtcgtttat gcgcagcgcg tgcgcttact tattaaaccg cagtctggaa aatcaccccg
    15841 tccgcttttt cggtgtactg agaaagctgg tcgaagttca gataacggta agtatcaacg
    15901 gctgttttat ctacctgcgc cacgtaggtc tggtactctt ccggcgtcgg cagtttgcca
    15961 atcagcgccg caacagcagc cagttccgca gaagccagga agacattcgc gccagtaccc
    16021 aaacggttcg ggaagttacg ggtagaggtg gaaaccaccg tcgcaccgtc tgctacacgc
    16081 gcctggttac ccatacacag ggaacagcca gggatctcga tacgagcacc actcttaccg
    16141 aagacgctgt agtagccttc ttcggtcagc tgcgcagcgt ccatacgcgt tggcggtgcc
    16201 acccacaggc gggtcggcaa ctgacctttg tgcgcatcca gcagtttacc agcagcacgg
    16261 aagtgaccga tgttagtcat gcaagaaccg ataaatactt cgtcgatctt ctcgccctgt
    16321 accgcagaca gcggacgtgc gtcatccggg tcgttcggag cacagaggat tggctctttg
    16381 atatccgcca gatcgatgtc gatcactgcc gcgtattccg catctgcatc ggcttccagc
    16441 agctcaggat tcgccagcca tttttccatg ccctgaatac gacgctccag ggtacgacga
    16501 tcgccgtaac cttccgcgat catccacttc agcaggacga tgttagagtt caggtattca
    16561 atgatcggtt ctttgttcag cttgatggta caaccggcag cagaacgctc ggcagacgca
    16621 tcggtcagct caaatgcctg ctcaactttc agatccggca gaccttcaat ttccagaatg
    16681 cggccagaga agatgttttt cttacctttc ttctcaacgg tcagcagacc ttgtttgatt
    16741 gcatacagcg gaatggcatg taccagatcg cgcagggtga tacccggctg cattttgcct
    16801 ttgaagcgta ccagaacgga ttccggcata tccagcggca ttacgcctgt tgcggctgca
    16861 aatgccacca gaccagaacc cgccgggaaa gagataccga tcgggaaacg ggtatgggag
    16921 tcaccaccgg taccgacggt atccggcagc agcatacggt tcagccagga gtggattacg
    16981 ccgtcacccg gacgcagcga cacaccgcca cggttcataa tgaagtccgg cagcgtgtgg
    17041 tgcgtgttca cgtcaactgg cttcggatac gccgcggtgt ggcagaaaga ctgcatcacc
    17101 aggtcagccg agaaacccag gcacgccagg tctttcagtt catcacgggt catcgggccg
    17161 gtggtatcct gagaaccaac agaagtcatt ttcggttcac agtacgcgcc cggacgaatg
    17221 cctttcacgc cacaggcacg gcccaccatt ttctgcgcca gcgagaagcc acggtcgctc
    17281 tcagcgacat ctttcgcctg acggaacaca tcgctgtgcg gcaggccaag tgcttcacgc
    17341 gctttggtgg tcaggccacg cccgataatc agcggaatac ggccgccagc acgcacttca
    17401 tcaatcagca cgtcggtttt cagttcgaag gttgccagca gttcgccggt ttcgtggtta
    17461 cgcacttcac ctttgtacgg gtaaacgtca attacgtcgc ccatgttcag gttagagacg
    17521 tcgacttcga ttggcagtgc acccgcgtct tccatcgtgt taaagaagat gggtgcaatt
    17581 ttaccgccga ggcacaaacc accgccgcgt ttgttcggca catgtggaat atcatcgccc
    17641 ataaaccaca gaacggagtt cgtggcggat ttacgcgatg aacccgtacc cacaacgtca
    17701 ccgacgtacg ccagcgggaa acctttctgt tgcagagctt caatctgttt gatcgggcca
    17761 acaacaccag gctgatctgg ctcaatacct tcacgggcgt ttttcagcat cgccagcgcg
    17821 tgcagtggga tatccgggcg tgaccacgca tctggtgccg gagagaggtc atcggtgttg
    17881 gtttcgccag tgactttgaa gacggtaacg gtcagttttt cagccagcgc cgggcgattc
    17941 aggaaccatt cggcatccgc ccaggactgc attacctgct tcgcatattc gttgcctgct
    18001 ttggctttct cttctacgtc atagaagtta tcgaacatca gcagcgtgtg agaaagtgct
    18061 ttggcagcga tcggtgccag tttggcatca tccagcgcgt cgatcagcgg atgaatgttg
    18121 taaccaccct gcatagtgcc cagcagttca atggcttttt ccggagtcag cagcggggat
    18181 ttagcttcgc ctttggcgac agcagccagg aagcctgctt tgacataggc ggcttcatcg
    18241 acgccagggg gaacacggtt ggttaacaga tctaacagga attcttcttc gcccgcgggc
    18301 gggtttttca gcagctctac aagtgcggcc atttggtttg catccagggg tttgggcgca
    18361 atcccctcag cggcacgctc agctacgtgc ttacggtatt cttctagcac gacggttctc
    18421 ctcgctctca ttgtcatagt gcggcaggcg attctcttca cgctcctgtg agacagcagt
    18481 ttgtagggta aatgcccagt accgcaacgg gcagaatagc aggattttga cgatgtgtta
    18541 atctgtttac aaaaaagcaa cataaaaaag tggctgaatc gttaaggata gtctagagaa
    18601 tgtttccctc cgtaaaaact tcgttaattc gcatggtgat aatcaccttt ctcaacgaat
    18661 cccaagatta gtcaaaattt aaactacggc ctctttatac tcggattcac agcacctgcg
    18721 ggtggcagtt cgccgaccat tgcgatttcc ttgagatccg aattatgaaa atgactttgc
    18781 cgtttaaacc ccatgtgctg gcgctaattt gcagtgccgg gctttgtgcc gcctctgccg
    18841 ggctatatat aaaaagccgc acagtggaag cgcctgtgga agcgcaatcg acacaacaga
    18901 ctgcgcctga catctccgca gttacgcttc ctgcaacggt ttccgtgcct cctgtaacac
    18961 ctgccgtcgt caaatctaca ttcagcactg cacaaatcga tcaatgggtc gcgcccgtcg
    19021 cgctgtaccc cgactctctg ctttcacaag tgttaatggc atcaacctat ccggcaaacg
    19081 ttgctcaagc agtgcaatgg tcacacgata atccacttaa acaaggcgat gctgctattc
    19141 aggcggtatc tggccagtcc tgggacgcca gcgtcaaatc actggtggcc tttccacagt
    19201 tgatggcgtt gatgggcgaa aacccgcaat gggtgcaaaa cctgggcgat gcttttctgg
    19261 cgcagccgca ggacgtgatg gactcggtac agcgattgcg gcaactggcg caacaaaccg
    19321 gctcgctgaa gtcatcaact gaacaaaaga tcatcacgac aacgaaaaaa gtcgtaccgg
    19381 taaatcagcc cgccaacgcc cctgccacac aatcaaatac cgtttctacg tccagccccg
    19441 tcgtcgcaga acctgcaccg accgtaataa ccattgagcc agccaatccg gatgtggttt
    19501 atattccgaa ctacaaccca aacgtggttt acggcagttg ggccaatacc gcttatccgc
    19561 cagtttatct gccgccgcca gccggagaac cgtttgttga cagctttgtg cgcggattcg
    19621 gctatagcat gggcgttgct accacatacg cactattcag cagcatcgac tgggacgacg
    19681 atgatcatga ccatcatcat catgacgatg atgattatca tcaccacgat ggcggtcatc
    19741 gtgacggtaa tgactggcaa cacaacggcg acaacatcaa tatcgacgtc aacaatttca
    19801 accgtatcac cggtgagcat cttactgata agaatatggc atggcggcac aatccaaact
    19861 accgtaatgg tatgccttat catgatcagg atatgacaaa acggtttcac caaaccgatg
    19921 ttaaaggcgg aatgagcgcc acgccgctac ctgctccaac acgcgacagc cagcgtcagg
    19981 cggcagcaag ccagtttcag caacgaacac acgccgcccc cgtcattacg cgagataccc
    20041 agcgtcaggc tgcggcacag cggtttaatg aagcggaaca ctatggcaat tatgacgact
    20101 tccgcgactt cagccaccac caaccactga cccagcagca aaaggacgcc gctcgtcagc
    20161 gttatcagtc ggcttctcct gagcagcgcc aggcagtccg cgagaaaatt caggctaacc
    20221 cgcagaacca gcagcgaaga gaagcggcac gtcagcgtat tcagtccgcg tcgcctgagc
    20281 agcgccaggc ggttcgcgag aaaatgcaga ctaacccgca gaatcaacag caaagagatg
    20341 cagcacgtca gcgtatccag tctgcctcgc ctgagcagcg tcaggttttt cgagagaaag
    20401 ttcaggagag ccgcccgcag cgtctaaacg acagtaacca tactgcccga ttgaataatg
    20461 agcaacggtc agcggtacgc gaacggttgt ctgagcgtgg agcaaggaga ctggaaaggt
    20521 aaattgcagg cgtaaaaaag cggcgttgtt agccgcttaa gactgatgac aaacatcgaa
    20581 tcgcccgatg cgcaagctta tcgggcctac ataagccatg caatatattg aatttgcatg
    20641 attttgtagg tcggataagg cgtttacgcc gcatccggca tcaaccacga gcactttatc
    20701 aacaatctga agcggccaga atagccgctt cttaatttgc aggatgttcc ggcaaacgaa
    20761 aaattacttc ttcttcgctt tcgggttcgg caggtcggta atgctacctt cgaacacttc
    20821 tgccgccagg cccacagact cgtgcagagt cgggtgcgca tggatggtca gtgcgatgtc
    20881 ttcagcgtca caacccattt cgattgccag gccgatttca cccagcagct caccgccgtt
    20941 agtaccgaca attgcaccac cgatcacacg gtgagattct ttgtcgaaaa tcagcttggt
    21001 cataccgtct gcgcagtcag aagcgatagc acgaccagaa gcagcccacg ggaaggtggc
    21061 ggtttcgtag ctgatgcctt tctctttcgc ttctttctca gtcagaccta cccatgcaac
    21121 ttctggttcg gtataggcga tggacgggat aactttcgga tcgaagtagt gttttttacc
    21181 ggcgataact tcagcggcaa cgtgaccttc gtgaacacct ttgtgtgcca gcattggctg
    21241 accgacgata tcgccgatag caaagatgtg cggtacgttg gtacgcagct gtttgtcaac
    21301 gcggatgaaa ccacggtcgt caacttccac gccagctttg cctgcgtcga ggtttttacc
    21361 gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa cgctgcggtt cagcgggtgc
    21421 ttttttgcct tccatcgtca cataaatgcc gtcttctttc gcttcaacgg cggtaacttt
    21481 ggtttccagc atcaggttga atttcttgct gatacgcttg gtaaagactt taacgatgtc
    21541 tttatcagct gccgggatga cctggtcgaa catttcaacc acgtcaatct gtgaacccag
    21601 cgcgtggtat acggtgccca tttccagacc gatgataccg ccacccatta ccagcaggcg
    21661 ttctggtact tctttcagtt ccagcgcgtc agtggagtcc cagatacgcg gatcttcatg
    21721 cggaataaac ggcagttgaa tcgggcgaga acccgctgca atgatcgcgt tgtcgaagtt
    21781 gatcacagtt ttaccgttct caccttcaac ttccagggtg ttagccccgg taaatttacc
    21841 cagaccgttg accactttga ctttgcggcc tttcgccata ccagccagac caccggtcag
    21901 ctgattgatc actttctctt tccaggtacg aatcttgtcg atatcggttt tcggttcgcc
    21961 gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg ataacttttg ctacgtgcag
    22021 cagtgcttta gaagggatac agccgacgtt caggcaaaca ccgccaagag tgttgtaacg
    22081 ttctacgatt acggtttcca gacctaaatc agcgcaacgg aaggcagcgg agtaacctgc
    22141 ggggcctgcc ccaagtacca cgacctgagt tttgatttca gtactcatca tgacctctat
    22201 atatttatct ccggcgggtc tgacgttttt tttatgacgc ccattatcca ccgggtcgtt
    22261 ctatccgtcg gtattttaca aaattgttaa caatttttaa acaacaaacg gcaaccgatt
    22321 tgtctattcg ctaataatct caatacattc atgagattac cagaaaaaag ccggccgttg
    22381 ggccggctct tttatttaca tcaccagacg gcgaatgtca gacagcgtgt tgttaatgat
    22441 ggtaatgaaa cgggcaccat cagcaccgtc gatcacgcgg tggtcgaagg agagagaaat
    22501 cggcagcatc agacgcggca cgaactcttt accattccac accggctcca tcgcggactt
    22561 ggaaacgccg aggatagcca cttccggcgc gttcacaatc ggcgcgaagt gggtagtacc
    22621 caggccgccg atgctggaga tggtgaagca accgccctgc atttcgcccg ctgtcagctt
    22681 accgtcacgc gctttcttag aaatagtcat cagctcgcga gacagctcga tgatgccttt
    22741 cttgttgacg tctttgaata ccgggacaac cagaccgttc ggggtatcca ccgccacacc
    22801 gatgttgatg tatttcttca gggtcagacg ctgaccgtct tccgacagcg aactgttgaa
    22861 gcgaggcatc tgctcaagag ctgcagcaac ggctttcatg atgaagacaa ccggggtgat
    22921 cttcacatcc agcttacgtt tcgccgcttc ttcgttctgc tgtttacgga acgcttccag
    22981 ctcggtgata tcggttttgt cgaagtgagt aacatgcggg atcatcaccc agttacggct
    23041 caggttcgca ccagagattt tctggatgcg gcccagttcc acttcttcga tttcaccaaa
    23101 cttgctgaag tccaccttcg gccacggcag catgcctggg ataccgccgc cagttgccgc
    23161 cggagctgct tctgcacgtt tgatagcttc tttcacgtaa gcctgaacgt cttcgcgcag
    23221 gatacgacct ttacggccgg tgcccttcac tttcgccagg ttaacgccaa actcgcgtgc
    23281 cagacggcgg atcagcggag tcgcgtgaac gtaagcgcca ttttcagcaa attcagattt
    23341 gccttcggct tttgcagccg gagctgctgc cggggcttca gcttttgctg ccggagccgg
    23401 cgctgccgct tcctgcttcg ctggagctgc cgcaggcgct gcgccttcaa cttcgaagat
    23461 cataatcagc gaaccagttt tcactttatc gccaacgttg actttcagtt ccttcacgac
    23521 gcctgcaaac ggagccggga cttccataga agctttatcg ccttctacgg tgatcagtga
    23581 ctgttcagcg gcaactttgt cgcccacttt caccatcact tcggtcactt caacttcgtc
    23641 accgccgata tccggaacgt taacttcttt catgccagct gctggtgcag gggccgctgc
    23701 cggagccgct tcctgtttag ccgccggagc tgccgcgcct gcttcacccg cgacttcgaa
    23761 gatcataatc agcgagccgg tagacacttt gtcacccacg ttcactttga tctctttcac
    23821 ggtgcctgca aacggagcag gaacttccat agaagccttg tcgccttcta cggtgatcag
    23881 cgactgttca gcttcaactt tatcgccaac tttcaccagg atttcggtca cttcaacttc
    23941 gtcgctgccg atatccggaa cgttaacgtc ttttgctgcc gcagccgctg gtgctgctgc
    24001 cggagctgct tctttcttct cttctgcctg agcaggtgca gcgtctgctg caccgtcggc
    24061 ggaatcgaaa atcataatca gtgcgccggt ctgggtttta tcgccaacag agactttgat
    24121 ctctttaacg atacccgcct gcggagatgg tacttccata gaggctttgt cgccttctac
    24181 ggtgatcagc gactgttcgg cttcaacttt gtcgcccact ttgaccagga tctcggtgat
    24241 ttcaacttca tcagccccga tgtccggtac tttgatttcg atagccatta ttcttttacc
    24301 tcttacgcca gacgcgggtt aactttatct gcatcgatgt tgaatttggc gattgcgtca
    24361 gcaaccactt tcttatcgat ttcgccacgt ttagccagtt cgcccagcgc cgcaaccacg
    24421 acataagaag catcaacttc aaagtggtga cgcaggttct cacggctgtc ggaacgaccg
    24481 aagccatcag tacccagtac gcggtagtcg tcagccggta cgtaagtacg gacctgctca
    24541 gcgaacagtt tcatatagtc ggtagatgcc actgccggag cgtcgttcat cacctgagcg
    24601 atatacggaa cgcgcggagt ttccagcggg tgcagcatgt tccagcgttc acaatcctga
    24661 ccatcacgcg ccaattcggt gaaggaagtc acgctataaa cgtcagaacc tacgccgtaa
    24721 tctttcgcca ggatctctgc cgcttcacgg acgtgacgca ggatagaacc ggagcccagc
    24781 agctgaactt tacctttgct accttcaatg gtttcgagtt tgtagatacc tttacggata
    24841 ccttcctcag caccttccgg cattgccggc atgtggtagt tttcgttcag cgtagtgatg
    24901 tagtagtaaa cgttctcttg tttttcaccg tacatacgct ccagcccgtc atgcatgatg
    24961 acagcaactt cgtaagcgta agccgggtcg taagagatac agttcgggat agtcagcgac
    25021 tgaatgtggc tgtgaccatc ttcgtgctgc agaccttcac cgttcagggt ggtacgaccg
    25081 gaagtaccgc cgatcaggaa gccacgcgct tgctggtcac cagccgccca gcacagatcg
    25141 ccgatacgct ggaagccgaa catcgagtaa tagatgtaga acggaatcat cggcagattg
    25201 ttggtgctgt aagaggtcgc cgctgccagc caggaacaac ctgcgcccag ctcgttgatc
    25261 ccttcctgca gaatctgacc tttctcgtct tctttatagt aagcaacctg ctcgcggtcc
    25321 tgcggggtgt actgctgacc gttcgggctg taaataccaa tctgacggaa cagaccttcc
    25381 ataccgaaag tacgcgcttc gtcggcgatg atcggtacca gacgatcttt gatcgacttg
    25441 ttcttcagca ttacgttcag ggcacgaacg aaagcgatag tggtagagat ctctttgctc
    25501 tgctcttcca gcagcgcgcc gaagtcttgc aggctcggca gctccagctt ctcggtgaag
    25561 ttcggctggc ggcttggcag ataaccgtgc agtttctgac gttgagcgtg cagataggta
    25621 tgctcttcag aaccttccgg gaaggtgatg tacggcagtt tttcgatatc agcatcagat
    25681 accggcacat tgaaacggtc gcggatatga cgcacaccgt ccatgttcat tttcttaacc
    25741 tggtgcgcaa tgtttttacc ttcagccgcg tcgcccatgc cataaccttt aatggtatga
    25801 gcaaggatta ctgtcgcttt gcctttggtt tcctgcgctt tcttgaatgc agcgtagatt
    25861 ttcttcggat cgtgaccacc acggttcagt gcccagatct gctcgtcagt ccagtctgca
    25921 accagtgctg cggtttcagg atatttaccg aagaagtgtt cgcgaacata cgcaccatct
    25981 ttcgatttga aggtctggta gtcgccgtca acggtttcgt tcatcagctg gatcagttta
    26041 ccgctggtat ctttacgcag cagttcatcc caacggctac cccacatcac tttaatcacg
    26101 ttccagccag caccttcgaa gatgccttcc agttcgttga tgatcttgcc gttaccggtg
    26161 accgggccgt caagacgctg caggttacag ttgataacga agaccaaatt atccagtttt
    26221 tcacgggtag cgatggtgat cgcacctttg gattccggtt cgtccatttc accgtcgccg
    26281 aggaacgcgt aaacggtttg tttagaggta tctttcaggc cacggtgttc cagatatttc
    26341 aggaatttag cctggtaaat agcaccaatc ggacccagcc ccatagatac ggtcgggaac
    26401 tgccagaatt ccggcatcag tttcgggtgc ggataggaag agaggccatt gccgtgaact
    26461 tcctgacgga agttatccag ctgctcctga gtcagacgac cttccaggaa agcacgagcg
    26521 taaacgcctg gggagatgtg gccctggaag taaaccaggt cgccgccatc ctgctcgttg
    26581 cgtgcacgga agaagtggtt aaagcacaca tcataaatgg ttgcggaaga ctggaaggac
    26641 gccatgtggc cgcccagttc gaggtctttt ttcgacgcac gcagcaccgt catgatggcg
    26701 ttccagcgga tagctgaacg aatacggcgt tccagttcca gattacccgg atactccggt
    26761 tgttcttcaa cggggatggt gttgatgtag ttgctgatac cggtgcctgc ggctacgttg
    26821 acaccgcctt tgcgggcttc agcaagcagt tggtcgatca gatactgagc acgctcaaca
    26881 ccttcttcac ggatgaccga ttcgatcgcc tggagccagt cgcgagtttc gatcggatcc
    26941 acgtcatttg ggaaacgttc tgacatgggt tattccttat ctatctaata acgttgagtt
    27001 ttctggaacc tgtcccattg aactctcgcc ggaaagctca ataagacagg ttctacgttt
    27061 agttgccgcg cttttatatg cgcttgattt acaacatctt ctggataatt tttaccagaa
    27121 aaatcactaa ttctttcgtt gctccagacg acgcagagaa cgctcacggc ggctctcttc
    27181 acggcttctg tcgagcaaaa tttcttcgat aaaggccaga tggcgatgcg atgcttcgcg
    27241 cgcttcttcc ggcttaccgg ccataatcgc ttcaaatatg cgggtgcggt gactactcac
    27301 cagcggcagc atctcgcgac gcgaatagag caattcgaag ttctggcgga cattctgggc
    27361 caacatcggc tccatacacc ttagcagatg aagcagaacc acattgtggg ccgcttcggt
    27421 gacggcaatc tgatactgga gtacggcgtt tgattccgcg tccagatcgc cagactgctg
    27481 cgccagctct atggcatggt ggagttcacg gatgcgttcc ttgtcttcat cggtactacg
    27541 cagcgcggcg taataagcgg cgataccttc cagggcgtgt cgtgtttcga gcaagtcata
    27601 ctgtgattca ggatggtcgg agagcagctc caccagcgga tcgctgaagc tttgccatag
    27661 gctgctctgg acaaaagtgc cgccaccctg gcgacgaagc aacaagccct tcgcttcgag
    27721 acgttgaatc gcctcacgca aggagggacg ggagacgtcg aactgttttg ccagttcacg
    27781 ttccggtggg agtttttcgc ccgggcggag agtgccttcg aggatcaaaa actccagttg
    27841 ctgctcaatc acatcggaga gttttggttg gcggattttg ctgtaggcca tgagttcctg
    27901 tcttaagcca cttgccgaag tcaattggtc ttaccaattt catgtctgtg acgctaaagt
    27961 aacaaagtat tcaccttatg tccatacagg ttttgattga aatcatgaaa ctgtgcacat
    28021 tttaacaact tgacatatat aacgtttcaa agctgtaact atgcacaaat gtagacttta
    28081 cgtaggaaag gtttttttta acgattatta aactataaaa atccgaattg aaccgattca
    28141 cttaccaatt tatcgatttt taattcata
//
LOCUS       DAZFJL010000056        27947 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFJL010000056 DAZFJL010000000
VERSION     DAZFJL010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.056"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(182..1357)
                     /gene="ubiF"
                     /locus_tag="ACHJ8C_002298"
     CDS             complement(182..1357)
                     /gene="ubiF"
                     /locus_tag="ACHJ8C_002298"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO5552149.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAQPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRAPTSLIALHRHNDLQELELKGGETILAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLIRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFMMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1503..2927
                     /gene="miaB"
                     /locus_tag="ACHJ8C_002299"
     CDS             1503..2927
                     /gene="miaB"
                     /locus_tag="ACHJ8C_002299"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO5552150.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDHSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3080..4120
                     /gene="ybeZ"
                     /locus_tag="ACHJ8C_002300"
     CDS             3080..4120
                     /gene="ybeZ"
                     /locus_tag="ACHJ8C_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO5552151.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4117..4584
                     /gene="ybeY"
                     /locus_tag="ACHJ8C_002301"
     CDS             4117..4584
                     /gene="ybeY"
                     /locus_tag="ACHJ8C_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO5552152.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEAIETEIMLALGYEDPYIAEKE"
     gene            4674..5552
                     /gene="corC"
                     /locus_tag="ACHJ8C_002302"
     CDS             4674..5552
                     /gene="corC"
                     /locus_tag="ACHJ8C_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO5552153.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5577..7115
                     /gene="lnt"
                     /locus_tag="ACHJ8C_002303"
     CDS             5577..7115
                     /gene="lnt"
                     /locus_tag="ACHJ8C_002303"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO5552154.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPSPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVTVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSLNGIQLTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(7242..9119)
                     /locus_tag="ACHJ8C_002304"
     CDS             complement(7242..9119)
                     /locus_tag="ACHJ8C_002304"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFO5552155.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSDESWRLLSSIFLHSSFSHLLMNMFALLAVGTVAERILGKWRLLI
                     IWLFSGIFGGLISACYALRESEQIVISVGASGAIMGIAGAAIATQLASGAGTHHKNQR
                     RVFPLLGMVALTLLYGTRQAGIDNACHIGGLIAGGALGWLSARLVGQNRFVTEGGIIV
                     AVTLLLTGAIWLVQQQIDESVLQVRQSLREAFYPQEIEQERRQKKQQLVEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSLDEKLIYATSFEANAVSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDEKTLLVPGAGRGNIVRINTITHQKEDFPAGDARGVVSAMGFRPKNGEIIFAD
                     SQGISRIRAEDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADDRKIFVMAKNRLIQMDRTRSLDPQAIIRHPQYGDVACIPE
                     P"
     gene            9573..10481
                     /gene="gltI"
                     /locus_tag="ACHJ8C_002305"
     CDS             9573..10481
                     /gene="gltI"
                     /locus_tag="ACHJ8C_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO5552156.1"
                     /translation="MQLRKPATAILALALSAGLVQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNAERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            10651..11391
                     /gene="gltJ"
                     /locus_tag="ACHJ8C_002306"
     CDS             10651..11391
                     /gene="gltJ"
                     /locus_tag="ACHJ8C_002306"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO5552157.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            11391..12065
                     /gene="gltK"
                     /locus_tag="ACHJ8C_002307"
     CDS             11391..12065
                     /gene="gltK"
                     /locus_tag="ACHJ8C_002307"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO5552158.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            12065..12790
                     /gene="gltL"
                     /locus_tag="ACHJ8C_002308"
     CDS             12065..12790
                     /gene="gltL"
                     /locus_tag="ACHJ8C_002308"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO5552159.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRNKAPARAKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIIEDS
                     PKDAFFDNPKSDRAKDFLAKILH"
     gene            12908..13843
                     /gene="rihA"
                     /locus_tag="ACHJ8C_002309"
     CDS             12908..13843
                     /gene="rihA"
                     /locus_tag="ACHJ8C_002309"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO5552160.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVASGAVKPLMRNLIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLCESEEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(13888..14370)
                     /gene="ybeL"
                     /locus_tag="ACHJ8C_002310"
     CDS             complement(13888..14370)
                     /gene="ybeL"
                     /locus_tag="ACHJ8C_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO5552161.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            14605..17187
                     /gene="leuS"
                     /locus_tag="ACHJ8C_002311"
     CDS             14605..17187
                     /gene="leuS"
                     /locus_tag="ACHJ8C_002311"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO5552162.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPIADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            17202..17783
                     /gene="lptE"
                     /locus_tag="ACHJ8C_002312"
     CDS             17202..17783
                     /gene="lptE"
                     /locus_tag="ACHJ8C_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO5552163.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            17783..18814
                     /gene="holA"
                     /locus_tag="ACHJ8C_002313"
     CDS             17783..18814
                     /gene="holA"
                     /locus_tag="ACHJ8C_002313"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO5552164.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSTHTPLRALFDKH
                     RVWQNRRGMMSEALNRLSQSQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            18816..19457
                     /gene="nadD"
                     /locus_tag="ACHJ8C_002314"
     CDS             18816..19457
                     /gene="nadD"
                     /locus_tag="ACHJ8C_002314"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO5552165.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSMQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            19481..20092
                     /gene="cobC"
                     /locus_tag="ACHJ8C_002315"
     CDS             19481..20092
                     /gene="cobC"
                     /locus_tag="ACHJ8C_002315"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO5552166.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARLVLSDRQHPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILVVSHQGVLSLLITRL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            20352..20669
                     /gene="rsfS"
                     /locus_tag="ACHJ8C_002316"
     CDS             20352..20669
                     /gene="rsfS"
                     /locus_tag="ACHJ8C_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO5552167.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            20673..21140
                     /gene="rlmH"
                     /locus_tag="ACHJ8C_002317"
     CDS             20673..21140
                     /gene="rlmH"
                     /locus_tag="ACHJ8C_002317"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO5552168.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            21171..23072
                     /gene="mrdA"
                     /locus_tag="ACHJ8C_002318"
     CDS             21171..23072
                     /gene="mrdA"
                     /locus_tag="ACHJ8C_002318"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO5552169.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            23075..24187
                     /gene="mrdB"
                     /locus_tag="ACHJ8C_002319"
                     /gene_synonym="rodA"
     CDS             23075..24187
                     /gene="mrdB"
                     /locus_tag="ACHJ8C_002319"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO5552170.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            24198..25286
                     /gene="rlpA"
                     /locus_tag="ACHJ8C_002320"
     CDS             24198..25286
                     /gene="rlpA"
                     /locus_tag="ACHJ8C_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO5552171.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVPVPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            25426..26637
                     /gene="dacA"
                     /locus_tag="ACHJ8C_002321"
     CDS             25426..26637
                     /gene="dacA"
                     /locus_tag="ACHJ8C_002321"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO5552172.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            26747..27010
                     /gene="ybeD"
                     /locus_tag="ACHJ8C_002322"
     CDS             26747..27010
                     /gene="ybeD"
                     /locus_tag="ACHJ8C_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO5552173.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            27111..27752
                     /gene="lipB"
                     /locus_tag="ACHJ8C_002323"
     CDS             27111..27752
                     /gene="lipB"
                     /locus_tag="ACHJ8C_002323"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO5552174.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         6784 a         7315 c         7200 g         6648 t
ORIGIN      
        1 cgaggttcga atcctcgtac cccagccaca ttaaaaaagc tcgcttcggc gagctttttg
       61 cttttctgcg tatattcaat gccgaatgcg atgttgactc gtcttatcct tcaaagtcgg
      121 atgcgacgct gccgcgtctt atccgaccta cggttggcat gcatccagca atgttgtgag
      181 gctacaaccc taacgcatat ttcagcgcct gacgtttcaa cacgccagca cgctccgccg
      241 ccatcaaacc aagattacgc acaaaacgca gcggtggcag attattgctg aatccggcat
      301 aaaacagatc cataccactt tgcatcatga agttatccgc catacgccgc atctggtaac
      361 gcttgagcac aggataactg gcccacgctt cgccgtagct gcgggcgttc accagaacgt
      421 caatcagggc atcgacatca cgataaccaa gattcactcc ctgtcccgcc agcggatgga
      481 tggtatgcgc ggcatcgccc accagagcaa gccctggttg cacatactgc aacgcatggc
      541 gacgtatcag cggaaacgca ccagcggcaa gcggtgtcac gtaacccaga cgcgacggga
      601 aatgcttcgc gatttccgcc tgtagctgcg ccatattcat attctgcaac tggcgaatgc
      661 gcgccggaga gtcataccac accagcgatg cccagttatc aaacaacggc agaaacgcac
      721 gcggtccgtc cggggtaaat tgctgccagg tgctgtcgcc gggatcgttc tcacactgta
      781 cgctaatcaa catacacgac tgcgcatact gccaggcatg aacgccaatt cccgccatct
      841 gtcgcacctg cgaatttgcg ccgtcggcac caatcaccag cttcgcgagg atcgtttcac
      901 cgcccttcag ttccagctcc tgaagatcat tatggcgatg cagtgcaatc agcgaggttg
      961 gcgcacgtaa cgttactttc ggatgcgctt ccagcgcctg ccacagcgcc tgttgcagga
     1021 cagtgttttc caccatgtag ccaagcaacg gtagcttaag ttcagcggcg tcaaacacca
     1081 catgcgccgt ttcccactcc cacgtttcca gtctgcggta aggatggcaa cgcatagcct
     1141 gtactgcatc ccagacccct aaccctttaa gcaatgatac cgaagccgca ctaatcgccg
     1201 agatccgcac gtccggctgg ctgtcagcga caaacggtgc aggttgggca tgttcgataa
     1261 ccgttaccga aaatccgtgc tgagccagtc ccagcgccag tgcgccgccg accattcctc
     1321 cgccgacaat ggcaatttcc gttggttgat ttgtcatggt caatcatcct gttagcaata
     1381 tgctttaagt ttacaggatt tttaccccca agggctgata acgctcatac tggtcacaac
     1441 ggcagcaaag cattacacta tgcgccctgc attcctggct actatttcgc aagagcaagt
     1501 cgatgaccaa aaaactccat attaaaacct ggggctgtca gatgaacgag tacgattcat
     1561 cgaagatggc cgatctgctg gatgccaccc acggctatca actgaccgac gtggcggaag
     1621 aagcggatgt gctgctgctg aacacctgct caatccgcga gaaggctcag gaaaaagtct
     1681 tccatcagtt gggtcgctgg aaactgttaa aagagaagaa tccagacctg attatcggcg
     1741 tcggcggctg cgtggcatcg caagaaggcg agcacattcg ccagcgcgcc cactatgtcg
     1801 atattatttt tgggccgcaa acgctgcacc gtctgccgga gatgatcaac tccgtgcgcg
     1861 gcgaccacag cccggttgta gatatcagct tcccggaaat cgagaagttt gaccgtctgc
     1921 cggaaccgcg cgccgaaggg ccgaccgcgt ttgtctccat catggaaggc tgcaataaat
     1981 attgcaccta ctgcgtagtg ccttacaccc gtggtgaaga ggtaagccgt ccgtccgacg
     2041 atattctgtt tgagattgcc cagcttgctg ctcagggcgt gcgtgaagtt aacctgctcg
     2101 gccagaacgt gaacgcctgg cgcggtgaga actacgacgg caccaccgga tcgtttgccg
     2161 atctgctgcg tctggttgcg gcgatcgacg ggatcgatcg tattcgcttt accaccagcc
     2221 atccgatcga attcaccgac gatatcatcg aagtgtaccg cgacacgccg gagctggtga
     2281 gcttcctgca tctgccggta cagagcggtt ccgatcgcat tctgaacctg atgggacgta
     2341 cccatacggc gctggagtac aaagcgatca tccgtaaact gcgtgcggcg cgcccggata
     2401 ttcagatcag ctctgacttc atcgtcggct tccctggcga aaccaccgaa gacttcgaga
     2461 aaacgatgaa gctgattgcc gacgtcaatt tcgacatgag ctacagcttt atcttctctg
     2521 cacgtccggg tacaccagcc gccgatatgg ttgatgatgt tccggaagaa gagaagaagc
     2581 agcgtctgta tattctgcaa gagcgcatta atcagcaagc gatggcatgg agccgccgga
     2641 tgctcggcac cacccagcgc attctggtag aagggacttc acgtaagagc atcatggagc
     2701 tttccggtcg cactgaaaat aaccgcgtgg ttaacttcga gggcacgccg gatatgatcg
     2761 gtaaattcgt cgatgtagaa attaccgacg tctacccgaa ctctctgcgc ggtaaagtgg
     2821 tgcgaactga agatgaaatg ggtctgcgcg tggcagaaac accggaatcg gtgattgccc
     2881 gtacccgcaa agaaaacgac cttggcgtgg gttattatca gccgtaattc tcaggcccgc
     2941 cgttccggtg ggccttgttt tttctctctt tatccccatc tttatcgcaa tgcttgcgcc
     3001 tttttcctgt ggcgtgaata atttccttat aggccggtga atgttcagct tccggcggca
     3061 tacagataag aggaacggtt tgaacataga cactcgcgaa atcactctgg agccagcaga
     3121 caacgcacgt ctgttgagcc tgtgcggccc gtttgatgac aacatcaaac aactcgaacg
     3181 ccgtctcggc atcgagatca atcgccgcga taaccacttt aaactgaccg gccgtccgat
     3241 ttgcgtcacc gctgcggcag acattctgcg tagcctgtat gtcgatactg ccccgatgcg
     3301 cggtcagatt caggatatcg aaccggaaca gatccacctt gcgattaaag aagcgcgggt
     3361 actggagcaa agcgcggaga gcgtgccgga gtacggcaaa gcggtcaata tcaaaaccaa
     3421 acgcggcgta attaagccgc gcacgccaaa ccaggcgcag tacatcgcca atattctcga
     3481 ccatgacatt accttcggcg ttggcccggc gggtacgggt aaaacctacc tggcagtggc
     3541 tgcggcagtt gatgccctgg agcgtcagga aattcgccgt attctgctga ctcgtccggc
     3601 ggtcgaagcc ggtgagaaac tgggcttcct gcctggcgat ttaagccaga aagtagaccc
     3661 gtatctgcgc ccgctgtacg acgcgctgtt tgaaatgctg ggctttgaga aagtcgagaa
     3721 actgattgag cgcaacgtta ttgaagtcgc accgctggcc tatatgcgtg gtcgtacgct
     3781 gaacgacgcg tttatcattc ttgatgaaag ccagaacacc accatcgaac agatgaagat
     3841 gttcctgacc cgtatcggtt ttaactcaaa agcggttatc accggcgacg tcacacagat
     3901 cgacctgccg cgtaatacca agtctggttt gcgtcatgcc atcgaagtgc tggccgatgt
     3961 cgaagagatc agctttaact tcttccacag cgaagacgtg gttcgtcacc cggtggtggc
     4021 gcgtatcgtt aacgcctatg aagcctggga agaagccgaa caaaaacgta aagcggcgct
     4081 ggcagcagaa cgcaagcgcg aagaacagga acaaaaatga gtcaggtgat cctcgattta
     4141 caactggcat gtgaagataa ttccgggtta ccggaagaga gccagtttca gacatggctg
     4201 aatgcggtga tcccgcagtt tcaggaagaa tcggaagtga ctattcgcgt ggtcgatacc
     4261 gccgaaagcc acagtctgaa tctgacctat cgcggtaagg ataagccgac caacgtgctc
     4321 tccttcccgt ttgaagtacc gcctggcatg gaaatgtcgc tactgggcga tctggttatc
     4381 tgccgtcagg tggttgagaa ggaagctcag gagcaaggca aaccactgga ggcgcactgg
     4441 gcgcatatgg tggtgcacgg cagtctgcat ttgttaggtt acgatcacat cgaagatgac
     4501 gaagcagaag aaatggaagc catcgaaaca gagattatgc ttgctctggg ctatgaggat
     4561 ccgtacattg ccgagaaaga ataatttgcc aggcatttga ctggcagacg tccccgccgt
     4621 tggtgattaa cgacggcgtt ttgattaact aacatgagaa cccatacgac gccatgagcg
     4681 acgacaattc acacagtagt gacacgataa gcaacaagaa gggatttttc tccctgttac
     4741 tcagccaact tttccacggt gaaccgaaaa accgtgacga actgctggcg ctgatccgtg
     4801 attccgggca gaacgacctt atcgacgaag atacgcgcga tatgctcgaa ggggtgatgg
     4861 acatcgcaga ccaacgcgtt cgcgacatca tgatcccccg ctcccagatg attaccctga
     4921 aacgcaacca gacgctggac gaatgccttg atgtcatcat cgagtccgcc cactcacgtt
     4981 tcccggtgat tagcgaagac aaagatcaca ttgaagggat tctgatggcg aaagacttgc
     5041 tgccgtttat gcgcagcgat gctgaagcct tcagcatgga caaagtgtta cgtcaggcgg
     5101 ttgtcgttcc tgaaagtaag cgcgtagacc ggatgctgaa agagtttcgc tctcagcgtt
     5161 accacatggc gatcgtcatt gacgaattcg gcggggtttc cggtctggtg accattgaag
     5221 acatcctgga actgattgtt ggtgagattg aagacgagta tgacgaagaa gatgatatcg
     5281 acttccgtca gctgagtcgt catacctgga ccgtgcgcgc actggcttct attgaagact
     5341 tcaacgaagc gttcggcacc cactttagcg atgaagaggt tgacactatc ggtggtctgg
     5401 tgatgcaggc atttgggcat cttccggcgc gtggcgaaac catcgacatc gacggttacc
     5461 agttcaaagt ggcgatggcc gacagtcggc gtattattca ggttcatgtc aaaatcccgg
     5521 atgactcacc ccagccgaag ctggatgaat aaaaccgaaa ctggatagat aactacatgg
     5581 cttttgcctc attaattgaa cgccagcgca ttcgcctgct gctggcgtta ttattcggtg
     5641 cctgcggaac gctggccttc tctccttacg acgtctggcc tgcggcgatt atttcgctga
     5701 tggggcttca ggcgttgacc tttaaccgcc gtccactcca gtctgccgct attggctttt
     5761 gctggggatt tggcctcttt ggcagcggta ttaactgggt ctatgtcagc atcgcgacct
     5821 ttggcggaat gcctagcccg gttaacatct tcctggtggt actgctggcg gcgtatttgt
     5881 cgctgtatac cggactgttt gctggcgtgc tgtcgcgtct gtggccgaaa accacctggc
     5941 tgcgcgtggc gattgccgcc cctgctctct ggcaagtgac cgagtttctg cgcggttggg
     6001 tgctgactgg cttcccgtgg ttacagttcg gctatagcca gattgatggc ccgttaaaag
     6061 ggctggcacc gataatgggc gtggaagcca ttaacttcct gctgatgatg gttagtggcc
     6121 tgctggcact ggcgttggtc aaacgcaact ggcgtccgct ggtggtgacc gtcgtgctgt
     6181 ttgcccttcc cttcccgctg cgttacatcc agtggtttac cccgcaaccg gagaaaacca
     6241 ttcaggtttc gatggttcag ggcgatattc cgcaatcgct gaaatgggac gaaggccagc
     6301 ttcttaatac gctgaagatt tactacaacg caacggcacc gctgatgggc aaatcatcgc
     6361 tgattatctg gccggagtcg gcgataaccg atctggaaat taatcagcaa ccgttcctta
     6421 aagctctgga cggtgagttg cgcgataaag gtagctcgct ggtcaccggg attgtcgacg
     6481 cgcgtctcaa taaacagaac cgctacgata cctacaacac catcatcaca ctgggtaaag
     6541 gcgcaccgta cagctatgaa tcagccgatc gctataacaa aaaccatctg gtgccgtttg
     6601 gcgagtttgt cccactggag tcgatactgc gtccgttagc accgttcttt gatttgccga
     6661 tgtcgtcatt cagccgcgga ccatatattc agccaccgct gtcgttgaat ggcattcaac
     6721 ttactgcggc catttgctac gaaatcattc tcggcgagca agtgcgcgat aacttccgcc
     6781 cggataccga ctatctgctg accatctcca acgatgcgtg gtttggtaag tctattggtc
     6841 catggcagca cttccagatg gcgcgaatgc gtgcgctgga gctggcgcgc ccactgttgc
     6901 gcagcaccaa caacggcatt acggcggtga ttggcccgca gggtgagatt caggcgatga
     6961 tcccgcagtt cacccgcgag gtgttgacca ctaacgtgac accaaccacc ggactcacac
     7021 cgtacgcacg aaccggcaac tggccgctgt gggtgctgac ggcgctgttt ggttttgccg
     7081 ctgtactgat gagtctgcgt gctcgcaaac gttaatgtgc ctgatgcgcg acgcttatca
     7141 ggcctacgcg atttttacaa tatgttgaat tttcgcgatt ttgtaggata aagcgtactt
     7201 tttcaacacg ctctggggca tgattatcat gccctttttt cttacggttc agggatacaa
     7261 gccacatcgc catattgggg atgccgaata attgcctgcg gatcaagcga gcgcgtcctg
     7321 tccatttgga ttaacctgtt cttcgccata acaaatatct ttctatcatc cgctgaaaaa
     7381 cgaagatgat tcacgtagct ggcgggataa acgccaataa tctgcccggc attaatattg
     7441 agcaggatga cataaccttg caggccatat gacaccaacg ctaaatactg accatccggg
     7501 ctaataccct caacggaata aacgctcctg ctacaccatt gcgtcataat gctggcttgt
     7561 tgatcctcag ctcttatacg tgaaatcccc tggctatcgg caaaaataat ctcaccattt
     7621 tttggtcgga atcccatcgc agaaacgaca ccacgcgcat cacctgcggg aaagtcttct
     7681 ttctgatgcg tgatggtatt gatccgcacg atgttacctc tgcctgctcc cggaaccagt
     7741 aatgtttttt catccgggga aagtgccata acccaggcgc ctggtttacc gctcgtcccc
     7801 gtcccgtcgt atttctccag tgggatatcc gccacatgct gataagtcac cagatcaatc
     7861 gctgacacac tattactggt gcgattcatc acccaggctt ttgtgccatc acgcgagagg
     7921 ataaggctgt caggatgggc gccggtggta atcgactgaa taatctcccc cgtcgccacg
     7981 ttaatgaccg atactgcatt cgcttcaaat gaagtcgcat aaatcaactt ttcatccagg
     8041 cttaatgcca gcgatctgac gccctgatct ttacagccac cacaatgttt gactgacttt
     8101 tctttcgcta tgggcgctgt aaaggtatgc aggattttct tttgtccgag gtcgaaaaca
     8161 acaatgctgt tcgtgttttc aattgcggca tacaacgtat taccatcccg actgatcgcc
     8221 atatcatgga tatcggcaat ctcagcgagt aaatcaccac tggcctgctc acgacttacc
     8281 ggagcggata atgtttccct gagggcgttg cgctcctcca ctaactgctg ctttttttgt
     8341 cgtcgctctt gttcaatctc ctgcggataa aacgcttcac gcaggctttg cctgacctgt
     8401 aaaactgatt catcaatctg ctgctgcaca agccagatag ccccggtcag aagaagagtg
     8461 accgcaacaa taatcccgcc ttccgtaacg aatcggtttt gcccaactaa acgcgcgctc
     8521 agccaaccca acgcgccccc cgcaatcagg ccgccaatgt ggcaagcgtt atctattcct
     8581 gcttgccggg taccatacaa cagtgtcagc gccaccatac ccaacagagg aaatactcgc
     8641 cgctggtttt tatggtgtgt gcccgcacct gaagcaagct gtgttgctat cgcagcgccc
     8701 gctattccca taattgcccc ggatgcccca acgctgatga ctatctgctc actttcgcgt
     8761 aacgcataac aggcgctgat cagcccacca aagatgccgg agaataacca gataatcagg
     8821 agtcgccatt tccccagtat ccgttccgct actgtcccca ccgccaggag tgcaaacata
     8881 ttcatcagca aatgggaaaa actactgtgc agaaaaatac tgctcaataa gcgccaggat
     8941 tcatccgaca acgtgagaga agcaacgtta gcgccgagat ggatgagtgc ggattgatcg
     9001 acttcttcgt aactcatcca ttgtgcgagg taacaaaata tcccaacaca gagcgcaaaa
     9061 aggatcaggg ttattgcggg taattgaaca tttaacggct taaccgaaga tgcagacata
     9121 aggtgattct tccttgaaaa gactagtaaa aagcattttc ccgtgtgtag ttatcatccc
     9181 ggcaaacgtc ccacaccgaa aagttaacac tgtccgaaat atgacttttt caaactgaaa
     9241 ttacaatcgt aattaattca cccctaagta ttttttggca tgtaagttgc tttatttact
     9301 gtgtaagcaa tgaaatcagc cgcaacgcaa ccgggttgca ccaccacaaa tcagcggtag
     9361 caccaaaaag gtgcggttta agaattttgc ttcggaatgg tgcggcgcac ctcgcaaaaa
     9421 taaacaataa aacagcaata tctttacatt tatccaaact aaatgttacc taacaaacac
     9481 aacactgcac aataaagtcg cagatgataa caacacaaac actcacaacg ggtatccatg
     9541 cgtttttaac gcagaagata aaggagttgg atatgcaatt acgtaaacct gccacagcaa
     9601 tcctggccct ggcgctttcc gcaggactgg tacaggcaga tgacgccgcc ccggcagcag
     9661 gcagcacgct ggacaaaatc gccaaaaacg gcgtgattgt cgtcggtcac cgtgaatctt
     9721 cagtaccttt ctcttattac gacaatcagc aaaaagtggt gggctactcg caggattact
     9781 ccaacgccat tgttgaagca gtgaaaaaga aactcaacaa accagactta caggtgaaat
     9841 tgattccgat cacctcacaa aaccgtattc cactgctgca aaacggcact ttcgattttg
     9901 aatgcggttc taccaccaac aatgccgaac gccaaaaaca ggcggctttc tctgacacta
     9961 ttttcgtggt cggtacgcgc ctgctgacca aaaagggcgg cgatatcaaa gattttgccg
    10021 acctgaaagg caaagccgta gtcgtcactt ccggcaccac ctctgaagtt ttgctcaaca
    10081 aactgaatga agagcaaaaa atgaatatgc gcatcatcag cgccaaagat cacggtgact
    10141 ctttccgtac cctggaaagc ggtcgtgccg ttgcctttat gatggatgat gctctgctgg
    10201 ccggtgaacg tgcgaaagcg aagaaaccag acaactggga aatcgtcggc aagccgcagt
    10261 ctcaggaagc ctacggttgc atgttgcgta aagacgatcc gcagttcaaa aagctgatgg
    10321 atgacaccat cgctcaggtg cagacctccg gtgaagcgga aaaatggttt gataagtggt
    10381 tcaaaaatcc aattccgccg aaaaacctga acatgaattt cgaactgtca gacgaaatga
    10441 aagcactgtt caaagaaccg aatgacaagg cactgaacta attacaagaa ccaggggcgg
    10501 aaattccagc cctctcgatt gttacgtagc acggacagac tatacgcctg gtggtcgttc
    10561 cccatcgggc ctgaaaaccg caatacgctg ggtaacaatc ttcgagggta gcagttaacg
    10621 ctgctaccct cttttttctg gagtagattt atgtctatag actggaactg gggtattttt
    10681 ttacaacaag ccccattcgg caacaccacc tatcttggtt ggatctggag tggctttcag
    10741 gtgacgatcg ctttgtcgat ctgcgcctgg attatcgctt tcctcgtcgg ttcatttttc
    10801 ggcattttac gtaccgttcc aaaccgtttt ctctccggtc tgggtacgtt gtatgtcgaa
    10861 ctgttccgca acgtgccgtt gattgtgcaa ttctttacct ggtacctggt gatcccggag
    10921 ctgctgccag agaaaatcgg tatgtggttt aaggccgagc tggaccctaa tattcaattt
    10981 ttcctttcat ccatgctctg cctggggctg tttaccgccg cccgtgtttg cgaacaggta
    11041 cgcgccgcta ttcagtcgct gccgcgtggg caaaaaaatg ccgcgctggc gatgggtctg
    11101 acgctaccgc aggcttaccg ctacgtactg ctgcctaatg cttatcgcgt tatcgtcccg
    11161 ccgatgacct cagagatgat gaacctggtg aaaaactccg ccatcgcctc gactatcggt
    11221 ctggtggata tggcggcgca ggcgggtaaa ttgctcgatt actcggccca cgcctgggag
    11281 tcgtttaccg ccatcacgct ggcgtatgta ttgattaacg cttttatcat gctggtgatg
    11341 acgctggttg aacgtaaagt tcgcctgccc ggcaacatgg ggggcaaata atgtacgagt
    11401 ttgactggag ttccattgtt ccttccctgc catatctgct cgacgggctg gtgatcacgc
    11461 tgaaaatcac cgtcacggca gtggtgattg gtattttgtg gggcaccatg ctggcggtga
    11521 tgcgcttgtc cagctttgcg cctgttgcgt ggtttgccaa agcctacgtt aacgtattcc
    11581 gctcgattcc tttagttatg gttttgctgt ggttttacct gatcgtaccg ggttttctgc
    11641 aaaacgtgct gggattatca cccaaaaatg atatccgttt aatttcggca atggtggcgt
    11701 tttcgatgtt tgaagcggcc tactattcag agattatccg tgctggtatt caaagtattt
    11761 ctcgtggtca gtcgagcgcc gcgctggcgt tgggaatgac tcactggcag tcgatgaaac
    11821 tgattattct gccgcaggcg ttccgcgcga tggtgccgct gttgctcact cagggcatcg
    11881 tactgtttca ggatacctca ctggtgtatg tgttatctct ggccgatttc ttccgtaccg
    11941 cctcaaccat tggtgagcgt gatggtacgc aggttgagat gatcctgttt gccggatttg
    12001 tttatttcgt tattagcctt agcgcgtcgt tgttggtcag ctacttgaaa agaaggacag
    12061 cataatgatt accctgaaaa atgtttcaaa atggtatggt cactttcagg tgctgaccga
    12121 ctgctcaacc gaagtgaaaa aaggcgaagt tgtggtggtt tgcggcccgt ctggttccgg
    12181 caaatcaacg ctgattaaaa ccgtcaacgg cctcgaaccg gtgcagcaag gtgaaatcac
    12241 cgtcgatggc atcgtggtta acgacaagaa aaccgatctg gcgaaactgc gttcccgcgt
    12301 cgggatggtg tttcagcatt tcgagctgtt cccccatctg tcgattatcg aaaacctgac
    12361 cctggcgcag gtgaaagtgc ttaaacgcaa taaagcgccg gctcgtgcaa aagcattgaa
    12421 attgctggag cgcgtcgggc tttccgcaca tgccaataag tttccggcgc agctttccgg
    12481 cggtcagcag cagcgtgtgg cgatcgctcg cgcattgtgc atggatccta ttgccatgct
    12541 gttcgacgaa ccaacgtcgg cgctggatcc ggagatgatc aacgaagtgc tggacgtgat
    12601 ggtcgaactg gcgaacgaag gaatgacgat gatggtggtg acccacgaaa tgggctttgc
    12661 ccgtaaagtg gcgaatcggg tgatctttat ggacgagggt aaaattatcg aagactcgcc
    12721 gaaagacgct ttcttcgata atccgaaatc ggaccgcgca aaagacttcc tcgcaaaaat
    12781 cctgcattaa tcactctggc gcgcatactc aattgcgcgc catctcacgc tttctcgttt
    12841 cgtccctcgt atctgttaca tcccggcgtt acccttgtcg caaagaaaca cacaacaagg
    12901 agcaacaatg gcactgccaa ttctgttaga ttgcgaccca ggtcatgacg acgctatcgc
    12961 aatcgttctc gccctcgcct caccagagct tgatgtcaaa gcaattacgt cttccgccgg
    13021 aaaccagaca ccagaaaaaa ccttacgcaa tgttctgcgt atgctgacct tgcttaatcg
    13081 caccgatatt ccggtagcaa gcggtgcggt aaaaccgtta atgcgtaatt tgattattgc
    13141 ggacaacgtg cacggcgaaa gcggtctcga cggcccggca ttaccggaac cggcatttgc
    13201 accgcaaaac tgcacggcag tggagctgat ggcgaaaacg ctgtgtgaaa gtgaggaacc
    13261 tgtcaccatt gtttctaccg gaccgcaaac taacgttgcc ttgctgctca atagccaccc
    13321 ggaactgcat agcaaaattg cccgtatcgt gattatgggc ggcgcaatgg ggcttggtaa
    13381 ctggacgcct gcggctgaat ttaatattta cgttgacccg gaagcggcag aaattgtctt
    13441 ccagtcagga attccggtgg tgatggccgg tctggatgtt actcacaaag cacaaatcca
    13501 cgttgaagac actgagcgtt tccgcgcgat tggcaaccct gtttcaacca ttgttgccga
    13561 actgctggat ttcttcctcg aatatcataa agacgaaaaa tggggctttg tcggcgcacc
    13621 actgcatgac ccatgcacca tcgcctggct gttgaaaccg gagttgttta ccactgttga
    13681 gcgctgggtt ggcgtggaaa cacaagggaa atatacccag ggtatgacgg ttgttgatta
    13741 ttattatctg actggcaata aaccgaatgc caccgtaatg gtcgatgttg atcgtcaggg
    13801 ctttgttgat ttactggctg atcgtctgaa attttacgct taatcactcg ccggatgaca
    13861 tcacgccatc cggcgccact catttcatta cggctcgaac gggcgtctct ggaactgatc
    13921 atgaccacat ttcgggcata gcgtcagcac ttccggtgta tagatcggga gatggaagtg
    13981 acatttctcg cagaccagat ttcccagccc taccacttct ccgctgtgat aaaccccgtg
    14041 atgattgagg tcctggaaaa cttcgcgcca ttcaagctgc gttttatcgg tgatgtctgc
    14101 cagctcctgc cacaagcttt ctttaatcac ccgcataaag acgctgtcag actcttcttt
    14161 caggctctct tcatagctca tggcgaactc ttccaggtca cgtctgacag ctcgcgtcag
    14221 ctcatcgacc tcggttcgcg ttaactcccc tgtttttatt acgcgctcgc gcgcctgttc
    14281 caccagtgcg tcgatatcac gttcgccatt gcgcaggcgt tcgctcagtg acgcgaccag
    14341 ttcacggtaa tattgagcaa ccttgttcat cgtttcatct cccgggtcgt taactgacta
    14401 taataataga cgttctttgt tccttgcttt gctaatacgc ccacaaactc tgtaaattaa
    14461 tgtatgaata atccgggcat catttcaacg tcgagtgcgc gaaaggctgt tttggcgcgg
    14521 gcgtttgggc tatgctatgc ggatctgaaa aaccacatca acgctacatt tgtagccgta
    14581 ttgaaaacag gaccactggc tgccatgcaa gagcaatacc gcccggaaga gatagaatcc
    14641 aaagtacagc ttcactggga tgagaagcgc acatttgaag taaccgaaga cgagagcaaa
    14701 gagaagtatt actgcctgtc tatgcttccc tatccttctg gtcgactaca catgggccac
    14761 gttcgcaact acaccatcgg tgacgtgatc gcccgctacc agcgcatgct gggcaaaaac
    14821 gtcctgcagc caatcggctg ggacgcattt ggtctgcctg cggaaggcgc ggcggtgaaa
    14881 aacaacaccg cgccagcacc gtggacgtac gacaacatcg cgtatatgaa aaaccagctc
    14941 aaaatgctgg gctttggtta tgactggagc cgcgagctgg cgacctgtac gccggaatac
    15001 taccgttggg aacagaaatt cttcaccgaa ctgtataaaa aaggcctggt atataagaag
    15061 acttctgcgg tcaactggtg cccgaacgac cagaccgtac tggcgaacga acaagttatc
    15121 gacggctgct gctggcgctg cgataccaaa gttgagcgta aagagatccc gcagtggttt
    15181 atcaaaatca ctgcttacgc tgacgagctg ctcaacgatc tggataaact ggatcactgg
    15241 ccagacaccg ttaaaacaat gcagcgtaac tggatcggtc gttccgaagg cgtggaaatc
    15301 accttcaacg ttaacgacta tgacaacacg ctgaccgttt acactacccg cccggacacc
    15361 tttatgggtt gtacctacct ggcggtggct gcgggtcatc cgctggcaca gaaagcggcg
    15421 gaaaataatc ctgaactggc cgcctttatt gacgaatgcc gtaacaccaa agttgccgaa
    15481 gctgaaatgg cgacgatgga gaaaaaaggc gtcgatactg gctttaaagc ggttcaccca
    15541 ttaacgggcg aagaaattcc cgtttgggca gcaaacttcg tattgatgga atacggcacg
    15601 ggcgcagtta tggcagttcc gggtcacgac cagcgcgact acgagtttgc ctctaaatac
    15661 ggcctgaaca tcaaaccggt tatcctggca gctgacggct ctgagccaga tctttctcag
    15721 caagccctga ctgaaaaagg cgtgctgttc aactctggcg agtttaatgg tcttgaccat
    15781 gaagcggcct tcaacgccat cgccgataaa ctgactgcga tgggcgttgg cgagcgtaaa
    15841 gtgaactacc gcctgcgcga ctggggtgtt tctcgtcagc gttactgggg cgcgccgatt
    15901 ccgatggtga cgctggaaga cggtaccgta atgccgaccc cggacgacca gctgccggtg
    15961 atcctgccgg aagatgtggt gatggacggc attaccagcc cgattaaagc agatccggaa
    16021 tgggcaaaaa ctaccgttaa cggtatgcca gcactgcgtg aaaccgacac tttcgacacc
    16081 tttatggagt cctcctggta ctatgcgcgc tacacttgcc cggagtacaa agaaggtatg
    16141 ctggattcca aagcggctaa ctactggctg ccggtggata tttacattgg tggtatcgaa
    16201 cacgccatta tgcacctgct ctacttccgc ttcttccaca aactgatgcg tgatgcaggc
    16261 atggtgaact ctgacgaacc agcgaaacag ttgctgtgtc agggtatggt gctggcagat
    16321 gccttctact atgttggtga aaacggcgaa cgtaactggg tttccccggt tgatgctatc
    16381 gttgaacgtg acgagaaagg ccgtatcgtg aaagcgaaag atgcggcagg ccatgaactg
    16441 gtttataccg gcatgagcaa aatgtccaag tcgaagaaca acggtatcga cccgcaggtg
    16501 atggttgaac gttacggcgc ggacaccgtt cgtctgttta tgatgtttgc ttctccggct
    16561 gatatgactc tcgaatggca ggaatccggc gtggaagggg ctaaccgctt cctgaaacgt
    16621 gtctggaaac tggtgtacga gcacacagca aaaggtgatg ttgcggcact gaacgtcgat
    16681 gcgctgactg aagatcagaa agcgctgcgt cgcgatgtgc ataaaactat cgctaaagta
    16741 accgatgata tcggccgtcg tcagaccttc aacaccgcaa ttgcggcgat tatggagctg
    16801 atgaacaaac tggcgaaagc accgaccgat ggcgagcagg atcgcgccct gatgcaggaa
    16861 gcgctgctgg cggtggtccg tatgcttaac ccgttcaccc cgcacatctg cttcacgctg
    16921 tggcaggaac tgaaaggcga aggcgatatc gacaacgcgc cgtggccgat tgctgacgaa
    16981 aaagcgatgg tggaagactc cacgctggtc gtggtgcagg ttaacggtaa agtccgtgca
    17041 aaaatcaccg ttccggtgga cgcaacggaa gaacaggttc gcgaacgtgc tggccaggaa
    17101 catctggtag caaaatatct tgatggcgtt actgtacgta aagtgattta cgtacccggt
    17161 aaactcctca atctggtcgt tggctaagcg cgggaggaag cgtgcgatat ctggcaacat
    17221 tgttgttatc tctggcggtg ttaatcaccg ccgggtgtgg ctggcatctg cgtgatacca
    17281 cgcaggttcc ttccactatg aaggtcatga tcctggactc aggcgatccg aacgggccat
    17341 taagccgtgc ggtgcgtaac cagttacgtc tgaatggtgt cgagttgctt gataaagaaa
    17401 ccacgcgtaa ggacgttcca tccctgcgtt tgggtaaagt gagcatcgcg aaagataccg
    17461 catcggtatt ccgtaacggt caaacagcag agtatcagat gatcatgacg gtgaatgcga
    17521 ccgtgttgat ccccggccgt gatatctacc cgattagcgc caaagtcttc cgttcgttct
    17581 tcgataaccc gcaaatggcg ttagcgaaag ataacgaaca agacatgatc gtaaaagaga
    17641 tgtacgaccg tgctgccgaa cagctgattc gtaagctgcc aagcatccgt gctgcggata
    17701 ttcgttccga cgaagaacag acgtcgacca caacggatac tccggcaacg cctgcacgcg
    17761 tctccaccac gctgggtaac tgatgattcg gttgtacccg gaacaactcc gcgcgcagct
    17821 caatgaaggg ctgcgcgcgg cgtatctttt acttggtaac gatcctctgt tattgcagga
    17881 aagccaggac gctgttcgtc aggtcgctgc ggctcaaggg ttcgaagaac accacacttt
    17941 ttccattgat cccaacaccg actggaatgc gattttttcg ttatgccagg ccatgagcct
    18001 gtttgccagt cgacaaacgc tattgctgtt gttacccgaa aacggaccga atgcggcgat
    18061 caatgaacaa cttctcacac tcaccggact tctgcatgac gatctactgt tgatcgtccg
    18121 cggtaataaa ttaagcaaag cgcaagaaaa tgccgcctgg tttactgctc ttgcgaatcg
    18181 cagcgtgcag gtgacctgtc agacaccgga gcaggctcag ctgccccgct gggttgctgc
    18241 gcgcgcaaaa cagctcaact tagaactgga tgacgctgca aatcaggtgc tctgctactg
    18301 ttatgaaggt aacctgctgg cgctggccca ggcactggag cgtttatcgc tactctggcc
    18361 ggacggcaaa ctgacattac cgcgcgttga acaggcggtg aatgatgccg cgcatttcac
    18421 cccttttcat tgggttgatg ctttgttgat gggaaaaagt aagcgcgcgt tgcatattct
    18481 tcagcaactg cgtctggaag gcagcgaacc ggttattttg ttgcgcacat tacaacgtga
    18541 actgttgtta ctggttaacc tgaaacgcca gtctacccat acgccactgc gtgcgttgtt
    18601 tgataagcat cgggtatggc agaaccgccg gggcatgatg agcgaggcgt taaatcgctt
    18661 aagccagtcg cagttacgtc aggccgtgca actcctgaca cgaacggaac tcaccctcaa
    18721 acaagattac ggtcagtcag tgtgggcaga gcttgaaggg ttatctcttc tgttgtgcca
    18781 taaacccctg gcggacgtat ttatcgacgg ttgatatgaa atctttacag gctctgtttg
    18841 gcggcacctt tgatccggtg cactatggtc atcttaaacc cgtggaaacg ctggcgaatt
    18901 tgattggtct gacgcgggtc acaatcatcc ctaataatgt tcctccgcat cgtccccagc
    18961 cggaagcaaa cagcatgcag cgtaaacaca tgcttgaact ggcgattgcc gacaagccat
    19021 tatttactct tgatgaacgc gagctaaagc gcaatgcccc ctcttacact gcgcaaacac
    19081 tgaaagagtg gcggcaggag caaggaccgg acgtgccgct ggcgtttatt attggtcagg
    19141 attcattgct gacctttccg acctggtacg aatacgaaac gatactcgac aatgcacatt
    19201 tgatcgtctg tcggcgtcct ggttacccac ttgaaatggc acaaccacaa tatcagcaat
    19261 ggctggagga tcatttgaca cataacccgg aagatcttca ccttcagcct gccggtaaaa
    19321 tttatctggc tgaaacgccg tggtttaaca tctcggcgac catcatccgc gaacgtttgc
    19381 aaaatggtga atcgtgtgag gatttattac cggaaccggt actgacttac attaaccaac
    19441 aaggcttgta tcgctgatac ctgtcgttac ggagggcatg atgcgactgt ggttaattcg
    19501 tcatggcgaa acgcaagcga atgtcgatgg tctgtacagc ggtcatgcgc ccactcccct
    19561 gaccgcgcgc ggtattgagc aagcgcaaaa tctgcatacg ctgctacatg atgtttcctt
    19621 tgacctggtt ttatgcagtg aactggaacg ggcacagcat accgcgcgac tggttcttag
    19681 tgaccgccag caccccgttc atatcatccc tgaactcaat gaaatgtttt ttggcgactg
    19741 ggagatgcga catcatcgtg acctcatgca agaagatgcc gaaaactata gcgcgtggtg
    19801 caatgactgg cagcatgcta tccccacgaa cggtgaagga tttcaggcat tttcgcaacg
    19861 tgtggaacga tttatcgcaa ggcttagtga atatcagcac tatcagaata ttttagtcgt
    19921 cagccatcag ggcgtactga gtctgttaat tactcgttta attggtatgc ctgccgagtc
    19981 tatgtggcat ttccgcgttg accaggggtg ttggagcgcc attgatatca accaaaaatt
    20041 cgcgacgcta cgcgtcctca atagccgtgc catcggggtc gagaatgcat gacttttctg
    20101 tttttttacg ggtaagccgc aacgaccatt gacagccccg ggcaggctga tattctccgc
    20161 agccagactt tttccgccag acacgacttt gtagaaattg ttttacaaaa atggcgatgc
    20221 aatctgcggc gcggggtggg atgatagccc actttcgaaa gccgattcgg cgacaattgt
    20281 cccgaaatcg cctctggttc aggtatactg acagaccatt tttatctatt tgattcaccc
    20341 agggggaaaa cttgcagggt aaagcactcc aggattttgt tatcgacaaa attgatgacc
    20401 tcaaaggtca ggacatcatc gccttagacg ttcagggcaa atccagcatc accgactgca
    20461 tgatcatctg tacgggtacg tccagccgcc atgttatgtc cattgctgat cacgttgtgc
    20521 aggagtctcg cgcagcgggc ctgttaccgc tcggcgtaga aggtgaaaac agcgccgact
    20581 ggattgtcgt ggatttgggc gatgtgattg tccatgtcat gcaggaagag agccgtcgcc
    20641 tgtatgaact ggaaaaactc tggagttaat gcgtgaagct gcaacttgtc gccgtgggaa
    20701 cgaaaatgcc ggactgggta caaaccggtt ttaccgagta cctgcgtcgt tttccgaaag
    20761 atatgccctt cgagctgatt gaaattccgg ccggaaaacg cggcaagaat gcggacatca
    20821 agcgcatact cgacaaagag ggtgagcaga tgttggcggc cgcaggcaaa aaccgcattg
    20881 tcaccctcga tattccaggc aagccctggg atacgccgca gttagccgct gagctggaac
    20941 gctggaagct ggatggtcgc gacgtcagtc tactgattgg cgggcctgaa gggttgtcgc
    21001 ctgcctgtaa agcggcggcg gagcagagct ggtcgctgtc ggcacttacc cttccccatc
    21061 cgctggttcg cgtgctggtc gcagagagtc tgtaccgggc gtggagcatc accaccaacc
    21121 atccttatca ccgtgagtga taagtgagct ttgagtagaa aacgcagcgg atgaaactac
    21181 agaactcttt tcgcgactat acggcagagt ccgcgctgtt tgtgcgccgg gcgctggtcg
    21241 cctttttggg gattttgctg ctgaccggcg tgcttatcgc caacctgtat aatctgcaaa
    21301 ttgttcgctt taccgactac cagacccgct ctaatgaaaa ccgcattaag ctggtgccta
    21361 tcgcacccag ccgcggcatt atctacgacc gtaacggtat ccctctggct ctcaaccgca
    21421 ctatctacca gatagaaatg atgccggaga aagtcgataa cgtgcagcaa acgctggacg
    21481 ctttgcgcag cgtggttgat ctgaccgatg atgatattgc ggcattccga aaagagcgcg
    21541 cacgttcaca ccgtttcacc tctattccgg tgaaaactaa cctgaccgaa gtacaagtag
    21601 ctcgctttgc cgtcaaccag taccgttttc cgggtgttga agttaaaggc tataaacgtc
    21661 gttactatcc ttacggttcg gcgttgaccc acgtcatcgg ctatgtgtcg aaaatcaacg
    21721 ataaagacgt cgaacgcctg aataatgacg gcaaactggc caactatgcg gcaacgcatg
    21781 atatcggtaa gctgggcatt gaacgttact atgaagacgt gctgcacggt cagaccggtt
    21841 atgaagaggt tgaagttaac aaccgtgggc gtgttatccg ccagttaaaa gaagtgccac
    21901 cgcaagccgg acacgatatt tacctgacgc tggatctcaa actccagcaa tatattgaaa
    21961 cgctgctggc gggtagccgc gcagctgtgg tagtcaccga tccgcgtacc ggtggggtgt
    22021 tggcgctggt ttccacgcct agttatgacc caaacctgtt tgttgacggt atctccagca
    22081 aagattattc cgccttgctg aacgacccga atacaccgct ggtgaaccgc gccacacagg
    22141 gggtttatcc tcccgcgtct acagttaaac cctatgtggc ggtttcggca ttgagtgccg
    22201 gggtgatcac gcgcaatacg acgctgtttg accccggctg gtggcaactg ccagggtcgg
    22261 aaaaacgtta tcgtgactgg aaaaaatggg gccacgggcg tctgaatgtc acaagatcgc
    22321 tggaagaatc tgcggatacc ttcttctatc aggtggccta cgatatgggg atcgatcgcc
    22381 tctccgaatg gatggggaaa ttcggttatg gtcattacac cggtatcgac ctggcggaag
    22441 aacgttccgg caacatgcct acccgcgaat ggaaacagaa acgctttaaa aaaccgtggt
    22501 atcagggtga caccattccg gttggtatcg gtcagggtta ctggacggcg acccctatcc
    22561 agatgagtaa ggcactgatg atcctgatta atgacggtat cgtgaaggtt ccgcatttgc
    22621 tgatgagcac cgccgaagac ggcaaacagg tgccatgggt acaaccgcat gaaccgcccg
    22681 tcggcgatat tcattccggt tactgggagc tggcgaaaga cggtatgtac ggtgttgcta
    22741 accgccctaa cggtacggcg cataaatatt ttgccagcgc accgtacaaa attgcggcga
    22801 aatccggtac cgctcaggtc ttcggtctga aagcgaacga aacctataat gcgcacaaaa
    22861 ttgccgagcg tttacgtgac cacaaactga tgaccgcctt tgcgccatac aacaatccgc
    22921 aagtggctgt cgccatgatt ctggagaacg gcggcgcggg tccggcggtt ggtacgctga
    22981 tgcgccagat cctcgaccac attatgctgg gtgataacaa caccgatctg cctgcggaaa
    23041 atccagcggt tgccgcagcg gaggaccatt aatcatgacg gataatccga ataaaaaaac
    23101 attctgggat aaagtccatc tcgatcccac aatgctgctg atcttactgg cattgctggt
    23161 ttacagcgcc ctggttatct ggagcgccag cggtcaggat attggcatga tggagcgtaa
    23221 aatcggccag attgcgatgg gtctggtcat catggtggtg atggcgcaaa ttcctccacg
    23281 cgtttatgaa ggctgggccc cctatctcta tatcatctgt attattttgc tggtggcggt
    23341 agatgctttc ggtgccatct ctaaaggcgc tcaacgctgg ctggacctcg gtattgttcg
    23401 ttttcaaccg tcggaaattg ccaagatagc cgtaccgttg atggttgcac gctttatcaa
    23461 ccgcgacgtt tgcccacctt cattgaagaa tacagctatc gcgctggtgc tgatatttat
    23521 gcccacgctg ctggtggctg cacagcctga cctgggaaca tcaatcctcg ttgcgctttc
    23581 cggcctgttt gtactgttcc tctctggcct tagctggcgt ctgattggcg tcgcagtagt
    23641 gctggtagcg gcgttcattc cgattctgtg gttcttcctg atgcatgatt accagcgcca
    23701 gcgcgtaatg atgctcctgg acccggaatc agacccactc ggcgcaggct atcacattat
    23761 tcagtctaaa attgctattg gctccggcgg attacgcggc aaaggctggc tgcacggcac
    23821 tcagtcacag cttgaatttc tccccgaacg ccataccgac tttatcttcg cggtactggc
    23881 ggaagagctg ggattagtgg gcattttgat tctgctcgct ctctacattc tgctgatcat
    23941 gcgcgggctg tggatagccg ccagagcgca aaccaccttt ggtcgcgtca tggctggcgg
    24001 cttaatgctg atattattcg tttatgtctt cgtaaatatt ggtatggtaa gcggtattct
    24061 gccggttgta ggggttccgc tcccactggt cagttatgga ggatcggcgc taattgtgct
    24121 gatggctggg ttcgggattg taatgtcaat ccacacccac aggaaaatgt tgtcgaaaag
    24181 cgtgtaagag gtgcgcaatg cgtaagcagt ggctcgggat ctgcatcgcg gcaggaatgc
    24241 tcgcggcatg tacaagcgat gatggtcagc aacagacggt aagtgtaccg cagcctgcgg
    24301 tatgtaacgg ccccatagtt gaaattagcg gggcagaccc gcgtttcgaa ccactgaacg
    24361 cgacggcaaa tcaggattac cagcgcgacg gtaaaagcta caaaatcgtg caggatccgt
    24421 ctcgatttag ccaggcggga ctggcggcaa tctatgatgc cgaacccggc agtaacctga
    24481 cggcctctgg cgaagctttc gatccgacac agttgacggc ggcccatcca acgcttccaa
    24541 tccccagcta cgccagaatc actaacctgg ctaacgggcg aatgatcgtg gtgcgcatta
    24601 atgatcgcgg cccttatggc aacgaccgcg tgatttcgct ttctcgcgcg gcagctgacc
    24661 gtcttaacac gtcaaacaac accaaagttc gtatcgatcc gattattgtc gcccaggatg
    24721 gttcgctttc tggtcctggt atggcttgta ccacagtcgc caaacagact tacgccctgc
    24781 cagcacctcc cgatttaagc ggtggcgcgg gaacaagttc agtgcctgtc ccgcagggtg
    24841 acattcttcc ggtcagtaat tcgacgctaa aaagcgaaga tccgaccggc gcgccggtaa
    24901 ccagcagcgg tttcctcggc gcaccaacga ccttagcgcc tggtgtactg gaaggcagcg
    24961 aaccgacgcc tgcgccacag cccgttgtta cagctccgtc gacaacgcct gcaacctcac
    25021 ctgcaatggt gacaccgcaa gccgcctcgc aaagcgccag cggcaacttt atggtgcaag
    25081 tcggggccgt tagcgatcag gctcgtgcgc aacagtacca acagcaactg ggacagaagt
    25141 tcggcgtccc cggtcgcgta actcaaaatg gcgcggtctg gcggatccag cttggcccat
    25201 tcgccagcaa agccgaagcc agtaccttgc agcaacgttt gcaaaccgaa gcccaattac
    25261 agtcatttat taccaccgcg cagtagcgta aagcaggcat ctgatgtgtc aatttctgta
    25321 aatgacgtta acaaagtcac gcggaaagtc agatgcctgc cggtagtgca tttgctatag
    25381 tagggcactt tttttaattc catcacggat gtcgtagttc agaccatgaa taccattttt
    25441 tccgctcgta tcatgaagcg cctggcgctc accacggctc tttgcacagc ctttatctct
    25501 gctgcacatg ccgatgacct gaatatcaaa accatgatcc cgggtgtacc gcagatcgac
    25561 gcggagtcct acatcctgat tgactataac tccggcaaag tactcgccga acagaacgct
    25621 gatgtccgcc gcgatcctgc cagcctgacc aaaatgatga ccagttacgt tatcggccag
    25681 gcaatgaaag ccggtaaatt taaagaaact gatttagtca ctatcggcaa cgacgcatgg
    25741 gccaccggta acccggtgtt taaaggttct tcgctgatgt tcctcaaacc gggcatgcag
    25801 gttccggttt ctcagctgat ccgcggtatt aacctgcaat cgggtaacga tgcttgtgtc
    25861 gccatggccg attttgccgc tggtagccag gacgcttttg ttggcttgat gaacagctac
    25921 gttaacgccc tgggcctgaa aaatacccac ttccagacgg tacacggtct ggatgctgat
    25981 ggtcagtaca gctccgcgcg agatatggcg ctgatcggcc aggcgttgat ccgtgacgta
    26041 ccgaatgaat actcgatcta taaagaaaaa gaatttacgt ttaacggtat tcgccagctg
    26101 aaccgtaacg gcctgttatg ggataacagc ctgaatgtcg acggcatcaa aaccggacac
    26161 actgacaaag caggttacaa ccttgttgct tctgcgactg aaggccagat gcgcttgatc
    26221 tctgcggtga tgggcggacg tacttttaaa ggccgtgaag ccgaaagtaa aaaattgcta
    26281 acctggggct tccgtttctt cgaaaccgtt aacccactga aagtaggtaa agagttcgcc
    26341 tctgaaccgg tttggtttgg tgattctgat cgcgcttcgt taggggttga taaagacgtg
    26401 tacctgacca ttccgcgtgg tcgcatgaaa gatctgaaag ccagctatgt gctgaacagc
    26461 agtgaattgc atgcgccgct gcaaaagaat caggtcgtcg gtactatcaa cttccagctt
    26521 gatggcaaaa cgatcgaaca acgcccgctg gttgtgctgc aagaaatccc ggaaggtaac
    26581 ttcttcggca aaatcattga ttacattaaa ttaatgttcc atcactggtt tggttaaaaa
    26641 ttgaacactt gaaagtgtaa tttccgtccc catatactaa gcatcagtaa aaaactcccg
    26701 ccttctggcg ggagttgcta tttaattacg ttacgccgga gctgacatga aaaccaaact
    26761 taacgaactg cttgaattcc ctactccttt tacttacaaa gttatggggc aggcgttacc
    26821 tgagctggtt gatcaggtgg ttgaagtggt acagcgccat gcgccaggtg actacacccc
    26881 aacggtaaaa ccaagcagca aaggcaacta ccactcggta tctatcacta tcaacgccac
    26941 tcatatcgag caggttgaaa cactgtatga agaactgggc aaaatcgata ttgtccgcat
    27001 ggttctgtaa ctcgcttctc cgttacccgg ctcctggtcg ggtaactccc ctctcctcgc
    27061 tgtgatatac ttttccccca cttttactca ttctccacgg agacgccgtt ttgtatcagg
    27121 ataaaattct tgtccgccag ctcggtcttc agccttacga gccaatctcc caggctatgc
    27181 atgaattcac cgatacccgc gatgatagta cccttgatga aatctggctg gtcgagcact
    27241 atccggtatt cacccaaggg caggcaggaa aggcggagca cattttaatg ccgggtgata
    27301 ttccggtgat ccagagcgat cgcggtgggc aggtgactta tcacgggccg gggcaacagg
    27361 tgatgtatgt gttgcttaac ctgaaacgcc gtaaactcgg tgtgcgtgaa ctggtgacct
    27421 tgcttgagca aacagtggtg aataccctgg ctgaactggg tatagaagcg catcctcggg
    27481 ctgacgcgcc tggtgtctat gtcggggaaa agaaaatttg ctcactgggt ttacgtattc
    27541 gacgcggttg ttcattccac ggtctggcat taaacgtcaa tatggatctt tcaccatttt
    27601 tacgtattaa tccttgtggg tatgcaggaa tggaaatggc taaaatatca caatggaagc
    27661 ccgaagcgac aactaataat attgcaccac gtttactgga aaatatttta gcgctactaa
    27721 acaatcctga cttcgaatat attaccgctt aattccatac atcaatggcc caatttacac
    27781 tgggtcatta ctcataatac ctgctaatgc tttacatctc cttcgcgttt gtatattttc
    27841 tttatcactt gcagaaatgc gatgctacgc cttgctccgt agcccaccta attgattaac
    27901 tcattgaaat aattgaccat ggtgagcaaa agcgttccgt cccaatt
//
LOCUS       DAZFJL010000057        27752 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFJL010000057 DAZFJL010000000
VERSION     DAZFJL010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.057"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..1208
                     /gene="dhaR"
                     /locus_tag="ACHJ8C_002324"
     CDS             <1..1208
                     /gene="dhaR"
                     /locus_tag="ACHJ8C_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO5552175.1"
                     /translation="NSQAQTLLNIDKESLIGQNIRKGFVFPPILNEAITQRNKLSQKQ
                     IVLECRGEFIELMVTLRPLSDGSFLLFLHPLDKIRKIAQQQISTNANFTFDSLHAASG
                     GMKQVLLIARRAIKSISPILINGEEGVGKLSLAMAIHNESEQRDGPFISVDCQMLSPE
                     NILHELLGSDVGPSPSKFELAHNGTLYLDKVEYLSGEVQSVLLKVLKTGLVTRSDSHR
                     LIPVRFRLITCTSSSLREYVQQGAFSRQLYYEISMNEIEIPPLRKRREDLKQMIDDII
                     DKYQERTRKKMTITPDANSVLLEYRWPGNISEFKNRMEKVFINCNRLVLGLENIPLDI
                     RQNNSSGDDDIPHLTSLAELEMQAIEHTCRVCEWNLTKAAEVLKIGRTTLWRKLKIYN
                     LYPNVEHAD"
     gene            1495..2865
                     /gene="ibeA"
                     /locus_tag="ACHJ8C_002325"
     CDS             1495..2865
                     /gene="ibeA"
                     /locus_tag="ACHJ8C_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000397940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative intracellular survival FAD-dependent
                     oxidoreductase IbeA"
                     /protein_id="HFO5552176.1"
                     /translation="MEFYLEPARNIPVLATTEVLVVGGGPSGIAAAMSAAREGAATML
                     IERFGCFGGMMTTAGVESIAWWRHENTVESGGLAREIEETAKSMGASSPEPQSNSQAI
                     NAERFKLVADAMLEQAGVRRVLHITAVDVIKQGNNLLGVITESKSGRQAILANVIIDC
                     TGDADIAWFAGAPFTKREREELMCMTTVFSCANINKNAFMQNIKSTEPKYGDWGADEE
                     NKNWSYDVHESCRDMFSPYLGKVFAKGKSAGIIPKDVTLGGSWSTVTEYGDANYLNVV
                     SIPAVDCTDVFDLTRAEIEGRKQAMQAIEALRQFQPGFEQAQLKNFGMTVGTRESRHI
                     IGRVQLTENDICNEGRHADSIGVFPEFIDGNGHLKLPLEANYFQIPYGVMIPQQVENL
                     LVCGRAIDADNFAYATIRNMGCCIVTGEGAGTAAAIAIKNNTTVSQVDIQTVQERLQQ
                     NGVKVF"
     gene            2880..4283
                     /gene="nhaC"
                     /locus_tag="ACHJ8C_002326"
     CDS             2880..4283
                     /gene="nhaC"
                     /locus_tag="ACHJ8C_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFO5552177.1"
                     /translation="MREKPSFYVALTPIIFMMIVLVVGIGVMGWPASVCLLISAAFSC
                     IIAMAKLKYTWDEIQGFIIDKISAVMAPVLIVIFVGFMVATWSYAGTLPMLVYYGMLL
                     VAPAWLYAIAFFLNAVLSYVSGASWGSVASIGVALMGIGSGLHADLPILAAAIVTGAY
                     FGDKLSPLSDTTNLTSAVTKTKLYDLIKYLLWTTIPSTIISLLFFTWLGMIHTSTGSI
                     SPESMQMLVQLKELYKFGLLPLLPMLIVLCFTFLRLPTVPALLSSAFVAVLVGWLYQG
                     FPLAEGIKATMSGFRLTMITDSTTLLPSVKELLEQGGLNNQGGFLSFIICAMMFAGIL
                     TGTGMMDVTLQSAANKIKSAFGAILASGVLAIIINLLTGSDGLNKIVISELMMKKFED
                     LNLSPLVLARTLEDFGTMSAPIIPWSAAGLYMATTLGVPTFSYLPYCVFCFCSMIFAL
                     IYASTGFRLLRFNEAKA"
     gene            complement(4370..5281)
                     /gene="hypT"
                     /locus_tag="ACHJ8C_002327"
                     /gene_synonym="qseD"
     CDS             complement(4370..5281)
                     /gene="hypT"
                     /locus_tag="ACHJ8C_002327"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO5552178.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(5346..6518)
                     /gene="iadA"
                     /locus_tag="ACHJ8C_002328"
     CDS             complement(5346..6518)
                     /gene="iadA"
                     /locus_tag="ACHJ8C_002328"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO5552179.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            complement(6531..6992)
                     /gene="yjiG"
                     /locus_tag="ACHJ8C_002329"
     CDS             complement(6531..6992)
                     /gene="yjiG"
                     /locus_tag="ACHJ8C_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO5552180.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            complement(6989..7672)
                     /gene="yjiH"
                     /locus_tag="ACHJ8C_002330"
     CDS             complement(6989..7672)
                     /gene="yjiH"
                     /locus_tag="ACHJ8C_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO5552181.1"
                     /translation="MTQQGDAIAGELATEKVGVKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGTGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            7922..8475
                     /gene="kptA"
                     /locus_tag="ACHJ8C_002331"
                     /pseudo
     CDS             7922..8475
                     /gene="kptA"
                     /locus_tag="ACHJ8C_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
     gene            complement(8488..9666)
                     /gene="yjiJ"
                     /locus_tag="ACHJ8C_002332"
     CDS             complement(8488..9666)
                     /gene="yjiJ"
                     /locus_tag="ACHJ8C_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5552182.1"
                     /translation="MPSSTHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASAMASGLLILAMAWL
                     LPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMILIALTLLMPSKKHAIVPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALISTAQLFKLQVVTS
                     "
     gene            complement(9734..10594)
                     /gene="yjiK"
                     /locus_tag="ACHJ8C_002333"
     CDS             complement(9734..10594)
                     /gene="yjiK"
                     /locus_tag="ACHJ8C_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO5552183.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNLYIVSEPNR
                     FYRFTPQSSH"
     gene            complement(10659..10916)
                     /gene="ytiA"
                     /locus_tag="ACHJ8C_002334"
     CDS             complement(10659..10916)
                     /gene="ytiA"
                     /locus_tag="ACHJ8C_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO5552184.1"
                     /translation="MKEYLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            complement(10913..11680)
                     /gene="yjiL"
                     /locus_tag="ACHJ8C_002335"
     CDS             complement(10913..11680)
                     /gene="yjiL"
                     /locus_tag="ACHJ8C_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO5552185.1"
                     /translation="MTYSIGIDSGSTATKGILLADSVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVIPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            complement(11690..12841)
                     /gene="yjiM"
                     /locus_tag="ACHJ8C_002336"
     CDS             complement(11690..12841)
                     /gene="yjiM"
                     /locus_tag="ACHJ8C_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO5552186.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLGPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            complement(12957..14237)
                     /gene="yjiN"
                     /locus_tag="ACHJ8C_002337"
     CDS             complement(12957..14237)
                     /gene="yjiN"
                     /locus_tag="ACHJ8C_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO5552187.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRIAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNL"
     gene            14422..15342
                     /locus_tag="ACHJ8C_002338"
     CDS             14422..15342
                     /locus_tag="ACHJ8C_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO5552188.1"
                     /translation="MTNFTTSTPHDALFKSFLTHSDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRQLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFNEFISEITRRLPQQRERIMTIAERIHNDGYIKGEQRILRLFLQNGVDPEW
                     IQKITGLSAEQIQALEQPLPDREHDSWLEY"
     gene            complement(15334..15640)
                     /locus_tag="ACHJ8C_002339"
                     /pseudo
     CDS             complement(15334..15640)
                     /locus_tag="ACHJ8C_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            15818..15982
                     /locus_tag="ACHJ8C_002340"
     CDS             15818..15982
                     /locus_tag="ACHJ8C_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO5552189.1"
                     /translation="MEFHENKAKAPFIGFVQLWQAVRSWWRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
     gene            complement(16024..16351)
                     /gene="symE"
                     /locus_tag="ACHJ8C_002341"
                     /pseudo
     CDS             complement(16024..16351)
                     /gene="symE"
                     /locus_tag="ACHJ8C_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534835.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
     gene            complement(16566..17234)
                     /locus_tag="ACHJ8C_002342"
                     /pseudo
     CDS             complement(16566..17234)
                     /locus_tag="ACHJ8C_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme endonuclease
                     domain-containing protein"
     gene            complement(17228..17401)
                     /locus_tag="ACHJ8C_002343"
                     /pseudo
     CDS             complement(17228..17401)
                     /locus_tag="ACHJ8C_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845607.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(17446..18612)
                     /locus_tag="ACHJ8C_002344"
     CDS             complement(17446..18612)
                     /locus_tag="ACHJ8C_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO5552190.1"
                     /translation="MKNYKVITPLFPTYAQVKAMMKAVSGYSLKAVRNMITAIHEQTG
                     TPQKPVDWSEPDLWISERLTGEDADIARRIWDTDNHILNPRHSYGCYLFLNYPQFELM
                     ESTPDDTWQPTSRGQKFLQDDEKTLRSLDDQEGILQLLELLAGREMSRRADLLPEWQA
                     FLHQHSKFASASSVKSTLYSRLYNLIDRDMVNREGMSYRITDTGRAWLGKALPTRKSD
                     PRKELLEAVKRYNEQQKELLREQISTMNPYKFEQLVGQLLEAMGYEQVEVTKASGDKG
                     VDVIGQVQVGITTITEVVQVKRMQNTITRPYIDQLRGALPYHKAIRGTLITTGKFAAK
                     CAEAALFPGAAPITLIDGDRLLELLIENNVGIRRSNAVELLDVDLQLFDELEMD"
     gene            18798..18899
                     /locus_tag="ACHJ8C_002345"
                     /pseudo
     CDS             18798..18899
                     /locus_tag="ACHJ8C_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18914..19216
                     /locus_tag="ACHJ8C_002346"
     CDS             18914..19216
                     /locus_tag="ACHJ8C_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HFO5552191.1"
                     /translation="MSMVKHKRGTSSTLDVQHEAELKVLANKFDEDIDYSDIPSSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRLGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(19250..20206)
                     /gene="yjiA"
                     /locus_tag="ACHJ8C_002347"
     CDS             complement(19250..20206)
                     /gene="yjiA"
                     /locus_tag="ACHJ8C_002347"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO5552192.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEVLCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(20217..20420)
                     /gene="yjiX"
                     /locus_tag="ACHJ8C_002348"
     CDS             complement(20217..20420)
                     /gene="yjiX"
                     /locus_tag="ACHJ8C_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO5552193.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            complement(20538..22688)
                     /gene="btsT"
                     /locus_tag="ACHJ8C_002349"
     CDS             complement(20538..22688)
                     /gene="btsT"
                     /locus_tag="ACHJ8C_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO5552194.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLKVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            23065..24729
                     /gene="tsr"
                     /locus_tag="ACHJ8C_002350"
     CDS             23065..24729
                     /gene="tsr"
                     /locus_tag="ACHJ8C_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO5552195.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQVE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVILVVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQREASAVVKTVTPATPRKMAVADSEENWETF"
     gene            complement(24772..26043)
                     /locus_tag="ACHJ8C_002351"
     CDS             complement(24772..26043)
                     /locus_tag="ACHJ8C_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO5552196.1"
                     /translation="MSWLLIIPLMIVCLFLNIRVYLAMFIGIIAYFLFFSSVPIEIAV
                     QRLIAPTQSPSLLAIPFFILLGTLMSYTGIAERILHIANVLVGKMRGGLGVANILVST
                     MMGGVSASNLADAAMLSRMMVPEMERKGYNRAFAAAITAGGSLVTPIIPPGIALIIYG
                     LVADVSIGKMFIAGLVPGLLCAVVLMFTVYLVARKTNAKPSRESWPTGKEVVFSLGQA
                     WPALFLIFAVVGGIRANIFTPTEAGAVAVLIVLAIGFFIYREMRISHVVKALGETARA
                     TASVMLVIMASAALGWIFSMEHAGVAVANFVTSLTENKYMFLLIINILLLTLGMFLEG
                     NAILLILVPLLKPVVEHFGIDPVHFGIIMIFNLSIGAFTPPVGTVMLLVCNITQVSVG
                     NFFKQSLPLLAALLLMLMLVTYIPAISLFLV"
     gene            complement(26040..26513)
                     /locus_tag="ACHJ8C_002352"
     CDS             complement(26040..26513)
                     /locus_tag="ACHJ8C_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO5552197.1"
                     /translation="MRLFGKVAEFSAAFALFVLVVVTIGAVFMRYFIGQPLQWTEEMS
                     GMLMIWVVMLGGVVAERDRAHLTIPFLMEMLPGKLRRVIAVLVALLSIALLLYMAWLG
                     YRLAEMAQFKVTQILKVSWFWIDLAVPVGALATAIYTLYWLINDTKQSDSGDKMQ"
     gene            complement(26577..27548)
                     /locus_tag="ACHJ8C_002353"
     CDS             complement(26577..27548)
                     /locus_tag="ACHJ8C_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HFO5552198.1"
                     /translation="MSKIIAMLITVSTFFLPSIIQAKPISIKVAYENNPGEPLDVVMR
                     YWADLLNKKSNGEITLALYPSSQLGSKQDVTEQAMMGMNVITLSDVAFLADYEPDLGI
                     LFGPYLTDDPQKLFKIYESDWFKQKNESLKKKGIHVVMNNYLYGTRQIISKKPIRTVD
                     DLAGLKIRVPNNVMQIKAIQAMGATPTPMPLGEVYPALTQGVIDGVENPISVLQGQKL
                     YEQARYLTMVNYLTNTSVWIGGEAFFSTLSPEQLEMIHQTGYEAGVYSQKLTLKRDAE
                     MLKTMQAAGVEVIYPDTGPFQKKAREVYSQFPEWTPGLYETIQQQLQ"
BASE COUNT         7142 a         6711 c         7033 g         6866 t
ORIGIN      
        1 ttaattctca agcacaaaca ctactgaata ttgataaaga aagtttaatt ggtcagaaca
       61 tccgaaaagg attcgtattt ccgccgattt tgaatgaggc aattacacaa cgaaacaaac
      121 tatcgcaaaa gcaaattgtc ctcgaatgcc gtggcgaatt tattgaactt atggttaccc
      181 ttcgcccgtt aagcgatggt tcgtttttgc tttttcttca tccattagac aaaatcagga
      241 aaatagccca acagcaaata agcactaatg caaattttac ctttgacagt ttacatgcgg
      301 cttcaggtgg tatgaagcag gtattactta tcgctcgccg ggcaattaaa tccatctctc
      361 cgattttgat caatggcgaa gaaggtgtgg gaaaattgag tctggcgatg gcaatacata
      421 atgagagcga gcaacgtgat gggccattta tttctgtaga ttgtcagatg ctatcaccag
      481 aaaatatctt acacgaactt cttggctctg atgttggccc ttcgccatcg aaatttgaac
      541 tggctcataa tggcacctta tatctggata aagtcgaata tctatcaggg gaagttcaga
      601 gtgttttatt gaaagtattg aaaacggggc ttgttactcg ctcagacagc catcgtttga
      661 tccccgtacg ctttcgcctg attacatgta ccagtagttc tttacgtgag tacgtgcaac
      721 aaggggcttt tagccgacag ctatattatg agatctccat gaatgaaatt gaaattccgc
      781 cattgcgcaa acgtcgtgaa gatctcaagc aaatgattga cgatattatt gataagtatc
      841 aggagcgcac tcgaaaaaaa atgacaatca cgcctgacgc aaattcagtt ctgcttgagt
      901 accgttggcc tgggaacatc tccgagttca aaaatcgaat ggagaaggta tttattaact
      961 gcaatcgact tgtcctcggg ttagagaata ttcctctgga tatccgacaa aataacagta
     1021 gtggcgacga tgatatccct catcttactt cactggcaga attggagatg caagctattg
     1081 agcatacatg tcgtgtctgc gaatggaatc taactaaagc agctgaagta ttaaaaattg
     1141 gtcgtacaac attatggcgc aaacttaaaa tctataatct ctatccaaat gttgagcatg
     1201 cagattgatg cttttcaaat tgaaataatt ttaatgtcgt agtgtttcat tttggtacag
     1261 tgctttaatg gtattgaaat attgtgatgt gctccataaa tgccaatgtg gttattagcc
     1321 agggagacgg aaaatgtgtt tacactcctg aaataataaa aaacaggcaa agagagatga
     1381 tctccttgta tgtctgtatt agcatgatgt tgcttgttaa taattaaaaa tatcaacgcg
     1441 cttatataag cgcgggggat tgttttactc aattattgaa tacggagata aagtatggaa
     1501 ttttatctgg aacccgctcg taatatacct gtgctggcaa ccacggaagt gttagttgtt
     1561 ggtggtggtc catcgggtat tgccgcagca atgagtgccg ctcgtgaagg cgcagctact
     1621 atgctgattg aacgtttcgg ttgttttggc ggaatgatga caacggctgg cgtcgagtca
     1681 attgcctggt ggcgtcatga aaatacggta gagtcaggtg gactggcacg cgaaatagaa
     1741 gaaacggcaa aatcaatggg ggcgtccagc cctgagccgc aatcgaatag tcaggctatt
     1801 aacgccgagc gtttcaaact ggttgcggat gcaatgcttg aacaggcagg tgtgcgccgc
     1861 gtactacaca ttaccgccgt tgatgttatc aagcagggca ataatttact cggcgtaata
     1921 acagagagta aatctggtcg tcaggctatt ttggcaaatg tcattattga ctgtactggc
     1981 gatgctgata ttgcatggtt tgccggagca ccatttacta agcgtgaacg cgaagagcta
     2041 atgtgtatga caaccgtttt tagttgcgca aatataaata aaaatgcgtt catgcaaaat
     2101 attaagagca cggaacctaa atatggagac tggggggcgg atgaagaaaa taaaaactgg
     2161 tcttatgatg ttcatgaatc ttgtcgcgat atgtttagcc cttatctggg taaagtcttt
     2221 gcgaaaggaa agtcggcagg aattattcca aaagatgtga cattaggcgg ttcctggagt
     2281 acggtcaccg agtatggtga tgcgaattat ttgaatgttg ttagcatccc cgccgttgat
     2341 tgtacagatg tttttgacct gacgcgtgca gaaatagaag gtcgcaagca agccatgcag
     2401 gcgattgaag cgttgcgtca attccagcca ggatttgaac aggcgcaatt aaaaaatttc
     2461 ggtatgacgg tgggaacaag agaatcaaga catattattg ggcgagtcca gcttacggaa
     2521 aatgatattt gtaatgaggg acgtcatgcg gattcaatag gggtattccc tgagtttata
     2581 gatggaaatg gtcatcttaa attacctctt gaagcgaact attttcaaat cccttatggc
     2641 gtaatgattc cgcagcaagt tgaaaacctg ttggtttgcg gacgggcaat cgatgcagat
     2701 aatttcgcct acgcgacaat ccgtaatatg gggtgttgta ttgtcactgg agaaggtgca
     2761 gggactgccg ctgctattgc cattaaaaat aacactaccg tttcacaggt agatattcag
     2821 acggtacagg aacgcttaca gcaaaatggc gtaaaagtct tttaacaggg aataaagtta
     2881 tgagagaaaa acccagtttt tatgtcgcgc ttacaccgat catttttatg atgatcgtgt
     2941 tggttgttgg catcggtgtt atgggctggc ctgccagcgt ctgtttatta atttcagcgg
     3001 cgttttcctg cattattgca atggctaagc tgaaatatac ctgggatgaa atccaggggt
     3061 ttattatcga taagatttcg gcggtgatgg caccagtact tatcgttatt ttcgttggct
     3121 ttatggtcgc gacatggagc tatgcaggta cactgccgat gctggtttat tacggtatgt
     3181 tactggttgc gcccgcatgg ctttatgcca tagcgttttt tctgaacgcg gtattgtcct
     3241 atgtttccgg ggcatcatgg ggatctgtcg cttctattgg tgttgcatta atgggcatcg
     3301 gttccggctt acatgctgat cttccaattc tggcggcagc cattgttacc ggggcttatt
     3361 ttggcgacaa actttcaccg ttaagtgata ccaccaacct gacttcagcg gtaaccaaaa
     3421 ctaaacttta tgatctgatt aaatatctac tctggacgac catcccatca acgattatta
     3481 gcctgctgtt ttttacctgg ctgggaatga ttcatacttc tacgggctct atttcaccag
     3541 aaagcatgca gatgctggta cagctcaaag agttatataa gtttggtctg ttaccgctac
     3601 tgcctatgct tattgttctg tgctttacct ttttgcgttt gcctacagta ccggcattat
     3661 taagcagtgc gttcgtggcc gtattggttg gatggctgta tcaaggtttc ccgctggccg
     3721 aaggtattaa ggccacaatg agtggtttcc gtctgacgat gatcactgat tcaacgacgc
     3781 tgttaccttc tgtgaaagaa ctgcttgaac aaggcggcct gaataatcag ggcggattcc
     3841 tgtcatttat tatctgcgcc atgatgtttg ctggcatcct gacgggaaca ggaatgatgg
     3901 atgtcacatt acaaagtgcg gcaaacaaaa taaaaagtgc ctttggcgct attctggcaa
     3961 gcggtgtttt agcgatcatc attaacttac ttaccggttc tgatggtttg aataagattg
     4021 ttatatctga attgatgatg aagaaattcg aagatcttaa tttatcaccg ttagtcttag
     4081 cccgaacgct ggaagatttt ggcaccatga gcgcgccaat tattccgtgg tcagctgccg
     4141 gattgtatat ggcgacaaca ctaggcgtgc cgacgtttag ttacttgccg tactgtgtat
     4201 tctgtttctg ctcgatgata tttgcgttga tctatgcttc tacaggtttc cgtttactgc
     4261 gcttcaacga agcaaaggcc tgagttattc aagataataa atgcgtttgc cggatggatt
     4321 aatctccatc cggcgttaat gtcaaaattc catgttttgc agaataatgt cagctaagca
     4381 caatctccag ctcgcgcagt tcacgccaga agcgttcggc aactggattc atgcgggtat
     4441 tcatccggta tgcataggcc tgaatcggga tcaccagttc gtcccgattc agtacaacga
     4501 gctgcccgct gcgaatttct tgttggatgg catactccgg caaccaggca attccacatc
     4561 cgtcgagggc aacctgcttt aaaagctcgc tcatcgaaga gacaaaaaag gtgctgaaac
     4621 tcaactcact gtggcgcgtc agagtgcgat taatcaatcg ccccatgtag gagttgcggc
     4681 tgtaattcag taacggaaag tgtggctgag cgaggttaaa aagtgcttct ccgtgttcgt
     4741 cactggcgca gacggggaac aattgagatt caaataagcg aatgtggtca aacggcgctt
     4801 ccagcaaatc ttcgtcgtga aaggaaaaaa tacagtcact ttgcccttca cgcagtttat
     4861 cgaccgcttc atcgacatca atggcttcaa tcgcccaggt aaagagcggc ggcatctggc
     4921 tgataatgga cggtaacaga ccgagggaaa gagagtgtgc agcggcaatc ttgattttac
     4981 gttgcgcgta atcgctgccg ccacgcagct ctgccaggtt gctttctaac tgttgcaaca
     5041 gatggcggat ctgcgaatga aagattttcc cttgttccga gagttgcagc ggcgtcacct
     5101 ggcggttaaa caattcaacg ccaatcgcct gttccagcgc gcggatgcgg cggctgaatg
     5161 ccggttgcga gacgttgcga ctgactgccg cctgggaaaa attgcggcat ttttccagag
     5221 tcagaaaatc ataaagccat ttggtttcaa tattatgcaa aaccgcacca cagtcatcca
     5281 ttacgtttta ctctgtcagc cgcccttgcg ggcattctac gtccattcgg gcggctgaca
     5341 accgtttaag ccgtttcaaa cgttccttta acgcaggctt tgccgtcttt gaccatcagt
     5401 ttgccgcgag cgtatacctg ctcaatgcgc aattctggcg tcatgaccag taagtctgcg
     5461 tcattgccag gcagaatttc gcctttcccg gtcaggttaa ggaaaccggc tacgctactg
     5521 gtgagcgggc gcagggcatc gctgatactg aaatcatagt ctttaaccag cacctgcacg
     5581 gtttccagca acgtttcaaa accggcaaca ccgatatggg ttaaattccc ttcgtcatcg
     5641 aagaacggct ggctaccgtt gccgtcggag ctgagggtga cgcgtgccag cggaataccc
     5701 gcctgaacgg cgcgggcaat accttcggca ggggcgaccg gttcgtcaat gctgctggtg
     5761 atatcgatgg tgccgccttt gcgcgcgaac tccagcgcct gctcaaacaa cggaacgttg
     5821 cggttaacgt gggtcggcag cagcttgctg atcggcacat cgcagttttc cagaaggtca
     5881 tagacaggct gtaacgcctt tttgctgtcg cccatgtgga acacggtgac gccaggtttg
     5941 ccgccgagca aaccgccaac gcgggattcc gccgccatat tagccaggtg ataaacgtcc
     6001 ggtgcggcag aacggtgatc agagatggcg catttcacac caatcacgcg atcgataatc
     6061 gccacgtctt tttccacgga acccgtaata gtgcgggaag ggacatgata agcgccggtc
     6121 agcatccagg cactgatgcc ttcttcattg agcgcacggg tcttggcgag cagggattcc
     6181 gggtggcgag agatagagtc ggtgcccagc agaccaacca ctgacgtgac gcccgcttcc
     6241 gtcagacgac ttaacgccac ttccggcgtg cgcgtcgtgg gacctgcttc gccgccacca
     6301 ccaatcaaat ggacgtgttg atcaataaaa cctgggcaga ggatctgccc gctgagatcg
     6361 acaaccgtgc agtccggtac aatgtcagaa gggatgttgc tggcaacggc aataatcttg
     6421 ccgttagcga cgaggacatc gcaaattccc cgatcttccg gcgcatacaa atgcgctccc
     6481 tgcagcaggg taaaaccggc tgcggtataa tcaatcatgg taactccttg ttaaacaata
     6541 agctgcataa cccagatcga cagcaatgcg ttaatcacgc agaccgtaat gatatgcgga
     6601 taatatttgg cattcacttc ggctgtcccc aggcagcgac cgacattttg caccgggttg
     6661 cccattaaat acatcgcggg cagtaaaacg gtgacatcgt gaccggttaa tgcgccggcg
     6721 gtcgccagac tggcggcaac accgactgcg ccgcccatac tcattaatgc ggctaaaagg
     6781 acggttgccg cttcaccggg taatccccac agcgccatta cgggttcgca gatatgcccc
     6841 acccagtcga gcaggccggt aattttcagc gcctgaatga tgacaaatgc catcaccacg
     6901 tttggcagca ggttggttgt ggcgatagta aagccacggc gcgcgccatc gataaacata
     6961 tccatgacgt ttttgcggac ctgagttgtc atgcttgcgc tccttgcgtt gggttgcgac
     7021 gttcttcgaa gttaagccag acgcgcaaaa tgttggctcc gacaaattta aacaccagga
     7081 tgacggctaa aggtacgatt acggacgtgc ccagaaaggc aaacactgcg acgccggaag
     7141 agaagtagtt ggtgatgatg gcgctaccgc tggtttgata ggcggcgaat atcactttat
     7201 cacgttcggt aatttcccct tcctgcgcca gttctttggt cataccggcg gcggcatcgg
     7261 tgttttgcag gttagcaatc agcgccagtg aacagatgcc tggaatgccc aacaacggtt
     7321 ttagcacggg cgtcattaac tgctgcgcgg cgcgcaatcc gccaagacca tcggtaatcg
     7381 agataattcc cagagagaga atcactgacg gagccagttc cagcgcgaag agaaagccat
     7441 ctttagctcc agtgccacct gcgccgcgaa acgaggtggt agcgccattc gcaccaggca
     7501 gttgcccaaa cgatccgttc agaacggaaa agtcaaaaac gcgccaccag ctgtctgttc
     7561 cggaaaaaac accggaaaaa aagattatgg tgagaaaaaa ggccagatac cctttaacac
     7621 ccactttttc tgtcgccagc tccccggcta tagcatcccc ttgttgtgtc ataacaatcc
     7681 ccattgtttt tttgtgtatg tgtgttgctt gagccaggaa aattcctgga gctgaaaaac
     7741 cttatcagtc atgtgggtat ggggaaaatg caagtatgtt tcttgctatg cgttttttgc
     7801 atagcggaaa aatgacgctg tgtggcaaag gttctgtgca ggattgagtt gcagaaaatt
     7861 cataaaccca gtgacaatga catcagtaaa taaaaacaga aaattcaggg acgaaaacgc
     7921 gatggcaaaa tacaacgaca aagagctggc agagacgagc aagtttttaa gttttgtcct
     7981 ccgacataaa ccagaggcaa ttggcatcgt gctggaccgt gaaggatggg cggatatcga
     8041 taagctcata ctctgtgcac aaaaagcggg caagcggtta acccgcgcat tactggatat
     8101 cgttgtcgcc accagcgata aaaagcgctt tagttattcc agtgatggta cgtgtattcg
     8161 cgccgttcag gggcatttga cttcgcaggt cgccattggc ttcatcgaaa aaacaccgcc
     8221 acaatttctc taccacggta cggcaagccg ttttcttgac gagataaaaa acaagggctg
     8281 attgcagggg agcgccatta cgtgcatctc tctgctgatg aagcaacggc acgcaaagtc
     8341 ggggcaagac acggatcgcc agttatttta accgtcaaag cacaggaaat ggcgaaacga
     8401 ggcatccctt tttggcaagc ggaaaacggg gtctggctga catcaacggt tgcggttgag
     8461 tttcttgagt ggtaagggaa acactcctca tgaagtgacc acttgcagct taaataattg
     8521 cgctgtgctg ataagcgcag caataaacaa cgccgccgcg ccgcagagag tggcgctggc
     8581 aagcgccgat gttccattgc cgagcatact ggttagcgct gggccgagga tttgcccaat
     8641 gccgtaaatg agcgtcacaa agcctaaaag attgagattt cccggtacgc tcagttggcg
     8701 ggcgatagtc atcaccagag atgtcgtgcc cataaatgta ccgccaaaac caagactgct
     8761 gatgataagc aagagaggcg agtcgctggc gagagtaagc aacacactaa tagcctgcac
     8821 cagcaaattc gccgttaggc agggcaaagc cccccaacgt tttgctgccc atagccagcc
     8881 aaagcagcct ggcacaattg ataagccgac taacgtccag agatgggcgg ttaacaatgg
     8941 tgagcctgca tctttcgcca tgagcggcag gtaagtcgcg acgatgatat aaccaaatcc
     9001 cgccaggccg tacagaatag ccagtaacca ccagctcatt atctgttgtt ccgtttttgc
     9061 caatggcatt ggtacgatgg cgtgtttttt cgagggcatt aaaagcgtaa gtgcaatcag
     9121 tatcatgcca gaaagcgccc cggcgccttg ccataacgtt tgcgaggaga ggtcaaaatg
     9181 caggcctgcc agaacatatt cattgcccag tgcgatgcca atgccaacgc cagaaaacaa
     9241 agctgccagc acaaaaggat ggcgcgtgtg ctgcataatc agcgtcgaac caaaaatcag
     9301 cattccggcg ctggcgacac ccgccaggac gcgaatcaat aacaccagaa taaatggcag
     9361 caaccatgcc attgcgagga tcaacaaccc gctcgccatg gcagaagcca acagaaatgg
     9421 gcgcaggcgc gatggctggt gaaatgcgcc aaaagaaaat agcagactgc cagccagata
     9481 ccccgcatag ttgccgctgg caatccacga gagctggcta aatgaaaacg atccttccgc
     9541 catcatgacg ggcaacatag gcgtataaag gaagcggcct aaacccatac ctaaggtcag
     9601 aaccagcatc ccgaaaagcg cggtgctgaa agaaaagcgt tctacgggat gcgtggacga
     9661 aggcatgttt aaccttgttt gtcatttttg ttgttaacaa gttaataaca aaaaggtgaa
     9721 acatgccagc ctgttaatgc gatgattgag gggtaaagcg ataaaaacga tttggttcgc
     9781 taacgatata aagattacca gaggcatcca tcgcaacccc ttcggcctgt ttgatgttat
     9841 gcgacaaacc tcgactgcct ttggttagtg acatttcacc gataacctcg ccaaccagcg
     9901 ttacttcctg gagtgcgcga gattcatgag acagcaccag aagcgtattt ttttgttggt
     9961 taaattctgc gccggacaca tcatctaaag taaattgccg ttgtagtgcc ttgtctttgc
    10021 tgatgtgcaa ttcattgcta cttaacagcc cgttaacttt gtagacctca atcgggtttt
    10081 tctctttaaa aaaccagaaa gtgtgatcct ggcgagaata ggccaatcct tcaaaaccgc
    10141 aattggttgg ggattcctgc agcgggattt ttattttttt gaggattttt acttccgagt
    10201 ttggggtcag tgaaatcaca taaatggcat aatcgcgctc atcgctaatg acaaactgat
    10261 tatcgccaat gtattcgatc gtctcgagat ccttaacaaa atccagagga attgtgcgga
    10321 tcaaatcgcc attggtggtc atttcaacaa tggcggcagg tttgttgata gtgctaaaaa
    10381 gagtattgct ttgtgccgac caggtcagcg aagagatatt attcgttatg cctgcaattt
    10441 ctttgccgtc aatggtggcg tggtaattct gaaatgatgc tgcatgattg cttttacggg
    10501 cgcaagattg cacgaagaaa gtacacactg ccacaatgac aaaaagaatc acaataacga
    10561 agatgcgttt acttaacgaa atactttttg tcacggtaat ccctttaagg caaatcaata
    10621 agatatcgag aagtatacgt agcgatctta atgaatcctt aacgcggaga aatcccgtaa
    10681 tgtgcaatgc agcgccagcc accatcctgc tgacaataaa ctgattccgt atgcccaggg
    10741 atcacccatt gaatttcctc gccaggcgga caggtcagcc aaatgcataa cccgcagccg
    10801 ccgcgtaaat cacggggaat atcgctgacg cgaaaagtca tgcccgctgc ctgcaacgcc
    10861 ttgcgggttt gtatgacgcc gaccgtggag tgaaataaaa acagatactc tttcatgcgc
    10921 gttttctctg ccgctgaccg atcaccgccg cgccaattgc accagcgaat tgcgcatcag
    10981 ggtgagtttg taccggcatt cccagatggc tttccagcat gtgggtaaac ctttgacagt
    11041 ggctaacgcc accagtaaac agaatcggcg cttcacagga gagacgagca atgaaattgg
    11101 cgctccgccg cgccatcgcg ttaatcactc cggcgagaat tgcttctggc gctacgcccg
    11161 cagagcgcag gctgatcact tctgattcag caaacactgt gcacatactc gtgatggcgt
    11221 gcggtatgac attttcggta atgctgtcga gttgctcgac gctggtgcca agcgtgcgcg
    11281 agatcacctc caggaaacgc ccggtgcccg ccgcgcattt gtcattcatc aggaaatcgc
    11341 acaggttacc gtcatcatca agctgaatca ctttgctgtc ctgaccaccg atgtcgatta
    11401 ccgcgcgcgt tgctggcgca agaaaccgtg cgcccagccc gtgacaggag atctccgtca
    11461 cttgtttatc ggcaaaatcc accagttgcc gtccgtagcc ggtgagcgtc agaaacggcg
    11521 ttgtctctaa cccttcgcgc agagtttccc aggcttcagt aattgctgtt gccgggcgaa
    11581 agggtgttgg aacgaggaaa cggcgcgtaa tcacgctgtc tgccagtaag atccctttgg
    11641 tggcggttga gccggaatca atgccaatcg aatatgtcac tgccactcct tacagcatct
    11701 caataaaggc cgcgacacgg gtgctgagct gcccgacatc cgaggtggag tagtctgttt
    11761 caatagcgat ataaggaatg ttgtgctgct ggcgcacatg acgtttaatc gccagcgatt
    11821 ccaccgcgta ggtatggcac gcctgcaaaa tcacatcaac tacgccatcg acctgatatt
    11881 cttcaaccat ctggctgagc atttgcaggc gctgatcgtt cggcgaaaca caggagcagc
    11941 caattgccag atacttatcc gccagcgcgt cgtacacatc acccgtttcc gccacgcatt
    12001 gctcggtggc tttcgccccg gtgcagtttt cataaccgac aacccagccg ccattctctt
    12061 caatcgcgcg caccactttc tctgctgcgc cgccaattgg gcagccggtg attaaaatac
    12121 gtgggcgcgg gcccagtcgc tggccttctt cccactgctg acgaacgcgg gcggtcattg
    12181 catccagttc attgatcaac gcctctttat cgaaccggaa ggttgcgccg tagactactt
    12241 tcagaatgtc gctgccgcta agcgccggag gatttaactg ccccaaatga taaaagttgg
    12301 ccagtgcgcg acgttcgcgg tttttcagcg caatggcatc gcgcagagca tcttcgctaa
    12361 tctcgtgccc aaaacgttct tctaccgttt tttgcaagcg cagcatctcg gctttccata
    12421 atgcacgcga ggcatcgtcc ttaacgctgt ttggcaactg catcacatga acgggcttaa
    12481 attctgccat gtattcatac attttctttt tgccgtcgca ggtggtttca ccgaccacca
    12541 gatcagaaaa gtagaagtag gggcatttat cggttttgcc gaagccgtag ctgcttttaa
    12601 tcagcgggca gaggttgcgc ggcagatctt tctcggcttc ttcaatggtt tcatcagagg
    12661 tggaacatag cgaaaccaca accgcaccgg ctgccatcgg gatctcttgc ggcataaagg
    12721 tgcagtaggt gccaaccagc ggaatgccgc gctccttgag atccatgacg gtgagaaagc
    12781 ctttctggcg agcttcagag aactgatcga aaatggcggg tagatcggtg acaagtgaca
    12841 tgattttcct tccccgtacc acgggagata atgaaaagag gccacattat taccattctt
    12901 ttagtgtggt ttatttgatc tctctcgcgg ttagccactt agtttttcat ggatttctat
    12961 aaattgccga gggggaacaa ggccgggagc tgcgacagca aatacaaaat aagtccaata
    13021 caaccgccaa ccagcgtacc gttgacacgg ataaactgga gatctttgcc gatatttaac
    13081 tcgatttgcc gcgacatatc ccgtgcgtcc cagcttttta ccgtgtcgct gatatggcga
    13141 gtcaggaatg cggaaaactc aggcgcaatg cggtgtgcgg cttgttccag atgaccattt
    13201 aacgacgccc gcaaggcatc atcggcaatt aacgtttcgc caaaccattg acccgcccgc
    13261 gcgatacgtt ctttcacacg agaatcttca ctgttgatat ccgctttcag ccattcccgt
    13321 aaatcccccc acaattcact gagatagcgg ttaaaagctt catcttcttt cagatagctt
    13381 tttacggcat cggctcgcgc tgccatttcc ggatcgtttt tcagcttgtc gatcagggca
    13441 aaagtggcgc gatcaaacgc atggcggatc tgatgcgcac gatcgcggct gatatcatca
    13501 agcaaagaat tcaccgcgtc agaaaccaac tccgcgctat gttcgcccag ccattcagtg
    13561 ggcaaaattt tggctttcag tggatgctcg ctctccagcc agcgaacaat ttgctgggca
    13621 ataaacttgc gtgatttatc gcgctggaga agggcgatca actgtgcgat cagcgtatcc
    13681 agcagcacct gatgacgatc gtttttggtc atactctcca gcatcaacgc actggtgcca
    13741 gaaagatcga ctttatcaat cgcccggtgg accgcgcgct taagcaggcg ctgaatacgc
    13801 gcatcatcgg tcagttcgag aaaaccgctc atgatctgta acagatgctg accaacgcgg
    13861 cgggcgttct ccggctgact aaaccagttg ccaatcaaca acgccggttc gtgacgtcga
    13921 atcaatgcca ccagcgattg ggtatcgaga aatttttcct gcacgaactg gccgagattt
    13981 tcgccaatcc ggtctttatt acgcgggata atcgccgtat gacgagaaat gatcggaatc
    14041 ggcacgcggc gaaacagcgc caccaccgca aaccagtccg ccagcgcgcc gaccatcgcc
    14101 gcttcagcaa tcgccttcac gccgctcacc caaaaattgg gcggcaaaaa cagcgtaacg
    14161 acaaaggtag cagcggcgat aagcagtaaa gagagcgcca acattttggc gcgtctgagt
    14221 tcaatgagtt tattcatatc gtaagaatag agcctgacgg taagaaaacg caaaaagcga
    14281 gatatcttcc ataaaaatgt atcgtttgca gaagaataaa aatagcctgg aaaccgcctc
    14341 ggggaatagg aaatagccag gtgtgaatgg ctttgttagt cgctaaagtt gggccatctt
    14401 atctacaggt gacggatcgc catgacaaac tttacgacca gcacgccgca tgacgcatta
    14461 tttaaatcct ttctcacaca ctctgacacc gcgcgggatt ttatggagat ccacttaccc
    14521 aaagatttac gtgaactgtg cgatctcgat agcttaaaac tggaatccgc cagctttgtc
    14581 gatgaaaaat tgcgggcgct acattccgat attctgtggt cggtaaagac ccgtgaaggc
    14641 gatggctata tttatgtagt gattgaacat cagagccgtg aggatattca tatggccttt
    14701 cgtctgatgc gatattccat ggcggtgatg cagcgccata tcgaacatga taaacgccgg
    14761 cagctaccgc tggtcatccc gatgctgttt tatcacggta gccgtagccc ttatccctgg
    14821 tctttgtgct ggctggacga atttgccgac ccgactaccg cacggaagct ttataccgcc
    14881 gcgttcccgc tggtggatat cactgtcgtg ccagacgacg agattgtgca gcatcgcaga
    14941 gtcgccctgt tggagttgat ccaaaagcat attcgccagc gcgatctgat ggggcttatt
    15001 gaccaactgg tagtattact ggttacagag tgtgctaatg acagccagat aactgcgctg
    15061 ttaaattaca ttttactgac tggtgatgaa gcgcgtttta atgagtttat cagtgaaatt
    15121 acccgtcgac tgccacaaca aagggagcga ataatgacga ttgcagagcg aattcataat
    15181 gatggatata taaaagggga acagcgcatt cttcgattgt ttttgcagaa tggggtagat
    15241 cctgaatgga tacaaaagat taccggactt tcggcagagc aaatacaggc attagagcag
    15301 cccttgcctg accgtgagca cgattcatgg ctcgagtact aatatggaac tatcgctatg
    15361 tgcctccagc atatccagca cctgatccca ctgggtaatt tccaccggac gcgtcatcga
    15421 cggtactggc ggctgggctt tacgttgcgc gacaaaataa cccgagcggc tacggagtga
    15481 tgaggttcat cgtctccagc gtctgatacg cctgctgcac ggtgctgacg ccgtgctcct
    15541 gactcaagct acgcaccgac ggcaatttct ccccgtgacg atacagccct tgctcaatcc
    15601 gctcggcaag cagagttgcc agatgttgat aacgcgtcat gctgtattcc ttatttggac
    15661 catacagaga gaaaaaccag tacagatgag gctaaaaaac cggatgcaga tgctgtttta
    15721 acctgtctgt atgttaaata aaactaatat ttgcatctgt attgtgaact gccagaagac
    15781 ggatgatgaa gcctctgaaa gatgaggaag gcagcagatg gaatttcacg aaaacaaagc
    15841 caaagcgccg tttatcggtt tcgtacaact ctggcaagcg gtgaggagct ggtggcggca
    15901 aatgcaggcc cgacgggtgt tacagcagat gagtgatgag cggttgaggg atattgggtt
    15961 acgcagggag gatgtggagt gagagggcaa ttccccttcc cgtatgggga aggggagaga
    16021 aacttagcgt ggcgttttgc tactaatcac cccaataaaa tcctgcacct gcttttgttt
    16081 acgcgccgac agcttgcaca cctggcgcag tgactgcatc agttcgctct cttcggcggc
    16141 gggtggttgg gcggtgagga caatacagcc ttccatcact ttgacatcta ccgccgtgcc
    16201 ggtggcaaaa ccggcggttt ccagccactg acctttcagg gtgatggcgg gaatacggct
    16261 gtaatccggg tagcggctcg cataactcac ggttatttgc cggggagact tctgcttcaa
    16321 acggttgtgc aatagaatgc gtgtcagtca tgattactat tctcgtgaaa tagggattgt
    16381 gatgagcggt gcgggtgtgt tggcgcacat tcgcaccgcg ctaaatacct ggatatatca
    16441 tcagtaaata cagggaaagt ccagctaaaa atagaaaata ataaaagcgt tactggaaag
    16501 atctggaaga gaaattaacg ttaattcaga atggccggat aacaaagtgt atccggtctt
    16561 cataattaat ttaaagcact catatgatgt tctttaaaat tctctgcctg tgccagcaca
    16621 cccatataaa catcatcatt cgctactggc gggaacttat gcttgtgtag aagcagagta
    16681 agttcaactt tcggtttcgc tttaatatca tcgcgtttac tccagtcagg atatttcgat
    16741 gtgttgtcaa caacgctttt catctctttt tctagtgaca gcattttttc atcgtcatag
    16801 gtgaactgat atttatcgcg catatgagca agaatgtcga aaaaggtttt ttcttcaata
    16861 tcaatgccta agtcggccca tgtgcccatt ttggttttaa tatcatagat aatatcagtc
    16921 atttcctgac tgaatgtatc gaattcttca ccgttgagta catcatcttc tcgccgctca
    16981 ttataacgat ctataatcgc ctggaagcgg ccggtgaagt taatcccttg caactggttc
    17041 actttcttga agtcgctgat cgctttttcc agtaattttt gtaataactg gatcttcgtt
    17101 gccggaagtt tgatcttgtt aattcgcgcc agataatctt cgtcaaaaat atcgacaaat
    17161 tccgcttttt tgtcgccaga aaaataaatt tcttccatcc catcagcttt taacgcttcg
    17221 gcaatcattt tttgcggcgt gcttcaaact tatcgctgat gagtttctgg agcacgaggc
    17281 ttaacaccac gtgcttgtat tcggaggact caacgctacc acgaaattgg tttgcggtat
    17341 cacataatgt ttcttcagag cttttctttg ctttggcttt tttagttggt gctttgacca
    17401 cgatgaactc cgtattgtaa tggtagaggc ggcagatttc gccctttaat ccatctccag
    17461 ctcatcaaac aactgcaaat ccacatccag caattccact gcattgctgc ggcgaatgcc
    17521 gacgttattt tcgattaaca gttccagtaa gcgatcgccg tcgataagcg taatgggcgc
    17581 agcccccgga aagagcgcag cttctgcgca tttcgccgca aatttcccgg tggtgataag
    17641 cgtaccgcga atggctttgt ggtaaggcaa cgccccgcgc aactggtcta tatacgggcg
    17701 agtgatagtg ttctgcattc ttttgacctg caccacttcg gtaatagtgg tgatgccgac
    17761 ctgcacctga ccaatcacgt ccacgccttt atcgcctgag gctttggtca cttcgacctg
    17821 ctcgtagccc attgcttcca gtaattgacc gaccagttgc tcaaatttgt acgggttcat
    17881 cgtgctgatt tgctcgcgca acagttcttt ttgctgctcg ttgtaacgtt tcactgcttc
    17941 cagcagctct ttacgcggat cgctttttcg tgtcggtaac gcttttccaa gccaggcgcg
    18001 cccggtatcg gtgatgcgat agctcatgcc ttcacggtta accatgtcac ggtcgataag
    18061 gttatagaga cgggagtaga gcgtgctttt cactgaactt gctgacgcga atttcgaatg
    18121 ctgatgcaaa aaggcctgcc attccggcaa taaatcggcg cggcgcgaca tttctcggcc
    18181 agccagcagc tcaagcagtt gcagaatgcc ttcctgatcg tccagtgaac gcagcgtttt
    18241 ttcgtcatct tgcaggaatt tttgcccgcg cgaggtgggc tgccaggtat cgtctggcgt
    18301 gctttccatc agctcaaact gaggatagtt gagaaataag tagcagccgt agctgtggcg
    18361 cgggttcaat atgtgattat cggtatccca aatacgacgg gcgatgtcgg catcttcacc
    18421 cgttaagcgt tcgctgatcc acaggtcagg ttccgaccag tcaacgggtt tttgcggcgt
    18481 accggtttgc tcgtgaatgg cggtaatcat attgcgtacc gctttcaggg aatagcctga
    18541 gacggctttc atcatagcct taacttgcgc ataggtagga aaaagcgggg taatgacttt
    18601 gtagtttttc acggtaatcc cttaacgaga gcgaggtgtt tagtgtgcca tgtcaggcga
    18661 gaaattctat tggatgaatc aagtccaact cactcattgt caatttcatg gttgattatc
    18721 caaaacgtga tgctatttcc aacatcaacg ctataattgt agatacattt ttgtatatac
    18781 aattagggat ggcaactatg ccaatggagt ttgaatggaa tgcaaacaag gccaaaagta
    18841 atcgggtgaa acatggcatc cgctttgaag atgcggtgtt agtgcttgat gatccacaac
    18901 gaacggagcc gctatgagca tggttaaaca taaacgaggg acctcctcta cactcgatgt
    18961 gcagcatgag gccgaactga aggtgctggc gaacaaattc gacgaggata ttgattacag
    19021 cgacatccca tcttccagcg atgagcaatg gtctaacgca gaacgcggta agttttatcg
    19081 gccattgaaa actcaggctt cggtgcgtat tgatgctgat gtgatggaat ggttgaagcg
    19141 actaggtaaa ggctatcaaa ctcgcctgaa cgcaatttta cgtgaagcga tgctgcgcga
    19201 tcagaacaaa aaataattca ggccgctaca tggcggccat catccaaaat tatttcctca
    19261 accccgcaaa cgccgcccga atctcttctt ccggcagctg aatcccgata aacaccatcg
    19321 tactatgcgg tttttcatcg ccccacgggc ggtcccagtc ggcgctgtag aggcgttgga
    19381 cgccctggaa cagcaggcga ttaggttcgc cgtcaatcca tagcatccct ttgtaacgca
    19441 gcagtttatc cgccgattcc agcagcaggt tttccatcac ccgggaaact tcgctgatat
    19501 ctaccgggta atccagttcc accacaatcg acgaaatatc gttttgttta tccgcgataa
    19561 aatggaaacg cggtttggtg ctgacgacgt tttcttccag cataaaaccg ttggtgttga
    19621 acagcaggcc caggtcgatg tcgccgtggg tgacggtgta gaccggtgca cgggcgttga
    19681 tgcgtgccag acgttcacgc agtttttctg cttcgcctgc gacgtcggtt ttagtcagca
    19741 gaatacggtc ggcgtagcca acctgtgact gggcgatggt gaactggttc atctgctcat
    19801 cggcatgtac cgcatccacc agcgcaatca ctccgtccag caggtaacgc tggcataaaa
    19861 cttcgtggga gaaaaaggtc tgaataatcg ggccgggatc ggccatgccg gtgcattcaa
    19921 taaccagacg atcgaactga atattgccct tgtcgagatt gtccagcagg tcgagtagcg
    19981 cgtcctccag ctcgttggag cgcgaacaac agatgcagcc gttggtcagc gttttgatct
    20041 gcgtggcgcg atcgccaatc agttgatcat caacggagac ttcgccgaat tcgttttcaa
    20101 tcacggcaat cttgtagccg tgttgttcgt taagaatatg gcgcagcagg gtggtttttc
    20161 ctgcgcctaa aaaaccggtg agtagggtaa ctgcaatcgg gttcatgctc tctccattaa
    20221 caacagcgca taccgccttt accatcgccg ccgtagcgcg cgttctggcg ttcgcggaag
    20281 aattcttcat agctcatgta cggcttgtcg ggatggttgg tcttcatatg ctcgacgtag
    20341 ttgtcgtagt ctggaatgcc aatcaacatc ttcgccgcct ggccgagata tttttttgcc
    20401 tgtcctaagt taccaaacat tttgccttcc agatatgcgt aaagcccgga aggtccgggc
    20461 tggggatttg ccggatgcgg cgtaaacgcc ttatccggcc tacgatagac attttgtaag
    20521 ccggataagg caaccggtta gtggtgcgaa gagatcttca cgccgccttc cgggattgga
    20581 acgtacggtg tttctttgtc agttcgtttg tcgctgttac gcaccttcag ccaggttttg
    20641 aaaccgtaga agatgatgct gtacaccaca atcaggaaca gaatactcag gcctgcgttg
    20701 gtgtagttgt tcacaacgat gtggttcatg ttggcaatct gctgcgccgt caggtcagta
    20761 ccgttagcaa tcttctcttt gtactggctt gccatgtaga agaagccttc catctgcggg
    20821 ttggtgctga acagtttcag gcccagcgcc caggtggtgc agataagcag ccatacagcc
    20881 ggaacaacag ttacccagat gtattgggtg cgcttcatct taatcagtac aacggtgccc
    20941 agtaccagcg ccacggctgc cagcatctgg ttggagatac cgaacagcgg ccacaggctc
    21001 ttaacgccgc ccagcggatc gaccacgccc tgatacagca ggtagcccca cagacccaca
    21061 cagcccgcag taccgatgat accggcaacc agggagtcgg tttttttcag gaacgggatg
    21121 aagttaccca gcaggtcttg cagcataaag cggccagaac gggtacccgc atccagcgcg
    21181 gtcaggatga acagggcttc gaacagaata ccgaagtgat accagaagcc catgtcagcc
    21241 atcggcagca ctttgtggaa cacgtgagcg atacctaccg ccagcgttgg tgcaccacct
    21301 gcacggttca ggacagaagg ctcaccaatg tctttcgcgg tttgcaggat ctgctctggc
    21361 gaaatcacga agccccagga gctgacggtc gctgccgcgt gtgcggtaac gtctttcagc
    21421 tgtgccatga tgatcggcgc gttctcgcca cccatttcat gcaggttagg catggtgatg
    21481 ccaaggccag caggcggggt gttcatcgcg aagtaaagac ccggttcgat gatggacgca
    21541 gcaaccagcg ccataatcgc cacgaaggac tccatcagca ttgcgccgta gccgatgaaa
    21601 cgcgcgtcgg tttcgttagc cagcagtttc ggcgtcgtac cggaagagat cagcgcgtgg
    21661 aagccagata ccgcaccaca ggcgatggtg atgaacagga acgggaacag agcgcctttc
    21721 cacagcgggc cagtaccgtc aatgtactgg gtcatggcag gcattttcag ttccgggttc
    21781 agcaccacga tacccagcgc caggccgacg ataacgccga ttttcaggaa ggttgccaga
    21841 tagtcgcgcg gtgcgaggat cagccacact ggcagcagtg cggaaacaaa cgcatagcca
    21901 atcagcgcga aggtaatggt ggtgtcttta aaggtcagtg ccggacccca gtacggatcg
    21961 tgtgcaatca cgccaccgaa gtagatagag gcaaccagca gcacgatacc aatgacagag
    22021 acttcaccca cacgccccgg acgaataaag cgcatgtaga tacccataaa cagcgcaatc
    22081 ggtacggttg agcaaacggt gaagacaccc cacggacttt cggccagggc tttaaccaca
    22141 atcagcgcca gaacggcgag gatgatgatc atgattaaga aacagccaaa cagcgcgata
    22201 gtccccggta ctggccccat ctcttctttg atcatctcac caagagatgc gccattacgg
    22261 cgagaggaga taaacagcac cataaagtcc tgaaccgcac cggccagcac gacccccgcc
    22321 agcagccaca gcgtgccagg caggtagccc atctgcgcgg cgagaaccgg acccaccagc
    22381 ggaccagcac cggcgatagc ggcgaagtgg tgaccaaaca acacgtaacg gttggtcgga
    22441 acgtagttca gaccgtcgtt gttaataacc gcaggcgtcg cgcgcgtggg gtcgagtttc
    22501 atcaccttct gggcgatgta cagactgtag tagcgatacg ccaccagata caccgacaca
    22561 gaggcgacca cgatccacag ggcgctgacg tgctcccccc gacgtaatgc aactaccgcg
    22621 aggcagaatg caccgatgat tccgagaatc acccagggta tgtgcttgaa tatcttttta
    22681 gtatccatag taaaacctgg catgtattga ttaatagttg gccgaagccg ttctaggttt
    22741 gcgttgcgtt tgaggaggta aattgaccgc tatcctgcag tgatgttgcc agagttacgc
    22801 gcgcgtaaag taaggtaaat aactgagtgg ttattttagg gacgtaagcg gtcaggaatg
    22861 gcggtgagcg gcaaaagcgt cgtttaagta accataaata tgtgatttat atcacatatt
    22921 tatattgcga ataatttatg taaataatgg ctttttaaat tcagggggtg aataaaatta
    22981 ctcggcgtca tcttcgcggg atattcataa agtttttcct ttccaggccg aaaatcttgc
    23041 atcggtccac aggaaagaga aaccatgtta aaacgtatca aaattgtgac cagcttactg
    23101 ctggttttgg ccgtttttgg ccttttacaa ctgacttcag gcggtctgtt ctttaatgcc
    23161 ttaaagaacg acaaagaaaa tttcactgtt ttacaaacca ttcgccagca gcaatccacg
    23221 ctgaatggca gctgggtcgc gttgttgcag acgcgtaaca ccctcaaccg cgcgggtatc
    23281 cgctacatga tggatcagaa caatattggt agcggttcaa ccgttgctga gctgatgcag
    23341 agtgccagta tttcgctgaa acaggtggaa aaaaactggg cggattacga agcgttgccg
    23401 cgtgacccgc gtcagagcac cgccgcagcg gcagagatca aacgtaatta cgatatttat
    23461 cacaatgcgc tggcggagct gatacaactg ttaggtgcag gcaaaatcaa cgagttcttt
    23521 gatcagccga cccagggata tcaggacggt ttcgagaagc agtatgtggc ttacatggag
    23581 caaaacgatc ggctctatga tatcgccgtc agcgataaca atgcctccta cagccaggcg
    23641 atgtggattc tggtaggcgt gatgatcgtc gtactggcgg tcatcctcgt cgtctggttc
    23701 ggtattaaag cctcgttggt cgcgccaatg aatcgcctga ttgacagcat tcgtcatatt
    23761 gcaggcggcg atctggtgaa accgattgag gtggatggct ctaatgagat ggggcaactg
    23821 gcagagagtt tgcgccatat gcagggagag ctgatgcgta ccgtcggtga tgtgcgcaac
    23881 ggggccaatg ccatttatag cggtgccagc gaaatcgcca ccggcaataa cgatctctct
    23941 tcgcgcaccg agcaacaggc cgcttcgctg gaagagacgg cagccagcat ggagcaactg
    24001 accgcaacgg tgaaacagaa tgccgaaaat gcgcgccagg ccagccatct ggcgttaagt
    24061 gcttccgaaa cggcgcaacg cggcggcaaa gtggtggata acgtggtgca gaccatgcgc
    24121 gatatctcca ccagttcgca gaaaatcgcc gatattatca gcgtaattga cggcattgcc
    24181 ttccagacca atattctggc tttgaacgcg gcggttgagg ctgcgcgtgc gggtgagcaa
    24241 ggacgtggtt ttgcggtggt cgcgggagaa gtgcgtaatc tggcccagcg tagcgctcag
    24301 gcggctcgtg aaattaaaag cctgattgaa gactcggtgg ggaaagtgga tgttggctct
    24361 acgctggtcg aaagcgccgg ggaaacaatg gcggagattg tcagtgccgt gacccgcgtg
    24421 acggacatta tgggcgaaat tgcttctgct tctgatgagc agagccgtgg tatcgatcag
    24481 gttggcttag cggttgctga gatggaccgg gtaactcaac agaacgccgc gctggtggaa
    24541 gaatctgccg ccgccgccgc cgcgctggaa gaacaggcca gtcgcctgac cgaagcagtg
    24601 gcagtgttcc ggattcagca acagcagcaa cagcagcgtg aagcatcggc tgtggtaaaa
    24661 accgtgacgc cagctacgcc gcgcaaaatg gccgtggcag atagcgagga gaactgggaa
    24721 acgttttaac gtaatgatgc cggatgagta tttcatccgg cgtttttatt attaaaccag
    24781 gaacagtgaa atcgccggga tatatgtcac cagcattagc attaacagta gagccgccag
    24841 taggggcagg gattgtttaa agaaatttcc caccgaaact tgcgtgatat tacataccag
    24901 cagcatcacg gtacctaccg gtggcgtgaa tgcgccaatc gacaggttga agatcatgat
    24961 gatgccaaaa tgcaccggat cgatgccaaa gtgttcgacc accggtttta atagtggtac
    25021 caggatcagc aaaatggcgt ttccttccag aaacatcccc agcgtcagca gcaatatgtt
    25081 gataatcaac aggaacatgt atttgttttc ggtcagcgaa gtcacaaaat tcgccactgc
    25141 gacaccagca tgttccattg agaaaatcca tcccaatgcg gcgctggcca tgattaccag
    25201 catcaccgat gcggttgcgc gtgccgtttc gcccaatgct ttgacgacat gagaaatccg
    25261 catttcacga tagataaaaa agccaatcgc cagcacgatt agcaccgcta ctgcacctgc
    25321 ttctgttggg gtgaaaatgt tagcgcgaat accacccacc acagcaaaaa tgaggaacag
    25381 cgcaggccag gcctgtccca gcgagaacac cacctctttg cccgtaggcc agctttcacg
    25441 cgagggttta gcgttagttt tgcgggcgac aaggtagacg gtaaacatca gtacgactgc
    25501 gcatagcaaa ccaggtacca gtccggcgat aaacattttt ccgatagaga cgtcggcaac
    25561 cagaccgtaa ataatcagag ctattccagg cggtatgatc ggggtgacta acgagccacc
    25621 agcggtaatg gcggcggcaa acgcgcgatt gtagccttta cgctccatct ccggcaccat
    25681 catgcgcgac agcatcgccg cgtcagcaag gttagaggcg ctgacgccgc ccatcatggt
    25741 gctgacgaga atgtttgcca cgcccagtcc gccgcgcatt ttccccacca gaacgttggc
    25801 aatgtgcagg atgcgttcgg caatgccggt gtagctcatt agtgtgccga gcaagataaa
    25861 aaacggaatt gccagcaatg atgggctttg cgttggcgcg attagtcttt gcacggcaat
    25921 ttcaatcggt acgctggaga agaataaaaa gtaagcgatg atgccaataa acatcgccag
    25981 atagacccga atgttgagaa acaaacagac gatcattagc ggaataatca ataaccaact
    26041 cattgcattt tgtctccgct atcggattgt tttgtatcgt ttattaacca gtagagggta
    26101 taaatagccg ttgccagtgc gccgacaggg accgccagat cgatccagaa ccaggaaacc
    26161 ttcagaattt gtgtcacttt aaactgcgcc atttcggcta gtcggtagcc aagccaggcc
    26221 atataaagca gcagtgcgat ggagagaagg gcgaccagta ctgcaataac acgcctgagt
    26281 ttgcctggaa gcatctccat caggaaggga atggtcaggt gcgccctgtc acgttccgcc
    26341 accactccac ctaacatcac cacccaaatc atcagcattc ccgacatttc ttctgtccat
    26401 tgaagcggtt ggccgatgaa ataacgcata aataccgctc caattgtcac cacaaccaga
    26461 acgaatagcg caaaggcagc actaaattct gccactttgc caaaaagacg catgaaacct
    26521 cctgttagtg cgtgtcctgc ctaatagaga tgaggcagga cgctgagcat taatgcttat
    26581 tgcagttgct gttgaatggt ttcgtacaga cctggtgtcc attccgggaa ttgagagtaa
    26641 acttcgcgtg ctttcttctg gaatggtcca gtatcgggat agatcacttc gacgccggct
    26701 gcctgcatgg ttttcaacat ttcggcatca cgcttaagag tgagtttctg gctatagaca
    26761 cccgcttcat agccggtctg gtggatcatc tccagttgtt ctggtgaaag ggtgctaaag
    26821 aaggcttcac cacctatcca cactgaggta tttgtcaggt agttaaccat tgtcagatat
    26881 cttgcctgct catacagttt ttgcccttgc agcactgaaa ttgggttttc tacgccgtca
    26941 ataacgccct gggttaatgc gggataaact tcgcctaatg gcatgggagt tggtgttgcc
    27001 cccatcgcct gaatggcttt tatttgcatg acgttattag gaacgcgaat ttttaaacct
    27061 gcgagatcgt caacggtgcg aatgggtttt ttcgaaataa tctgtcgagt gccatagaga
    27121 taattattca ttaccacatg aattcccttt ttcttcagac tttcattttt ttgtttaaac
    27181 cagtcgcttt catatatttt gaacagtttt tgcggatcgt cagtcagata cggtccaaac
    27241 aaaatcccca aatcgggttc ataatcggca agaaaagcca catcactcaa ggttatgaca
    27301 ttcatgccca tcatggcttg ctcggtgaca tcctgttttg atcccaattg ggagctggga
    27361 tagagcgcaa gtgttatttc gccattactt tttttattta ataggtctgc ccagtaacgc
    27421 atgaccacat ccaggggttc tccagggtta ttttcatagg caacttttat tgaaataggt
    27481 tttgcctgga ttattgacgg taaaaaaaag gtactgacag taattagcat cgctattatt
    27541 ttgctcatct ttacttactc catgctaagg aaataaaact ccaacattgt tgtcgggagt
    27601 gaaaccttat gaaaaagcag tcattactga aaagtagtca gcgaatagca gaatggctgc
    27661 agatgtatca taaattcgtg caccgggtaa tactttgctg cttttaatcg gtttttaatg
    27721 cattttttga ttgtatatct taaagttatg ta
//
LOCUS       DAZFJL010000058        27716 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFJL010000058 DAZFJL010000000
VERSION     DAZFJL010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.058"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            69..1793
                     /gene="yhgE"
                     /locus_tag="ACHJ8C_002354"
     CDS             69..1793
                     /gene="yhgE"
                     /locus_tag="ACHJ8C_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO5552199.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNLSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMTPWRVEKALWD
                     FGYYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLATALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTADRLQGVLAVLVLLVWSLGY
                     FVSIVWRKGQNPLDLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LADNVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     NDDRVIVYGFDSARKEWDALDMSLLPRQITKEKLLAAAKDGKLGTRPKAWRDLVVDGE
                     RLDVNLNE"
     gene            complement(1856..2734)
                     /gene="hslO"
                     /locus_tag="ACHJ8C_002355"
     CDS             complement(1856..2734)
                     /gene="hslO"
                     /locus_tag="ACHJ8C_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO5552200.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(2759..3160)
                     /gene="hslR"
                     /locus_tag="ACHJ8C_002356"
     CDS             complement(2759..3160)
                     /gene="hslR"
                     /locus_tag="ACHJ8C_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO5552201.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(3171..3839)
                     /gene="yrfG"
                     /locus_tag="ACHJ8C_002357"
     CDS             complement(3171..3839)
                     /gene="yrfG"
                     /locus_tag="ACHJ8C_002357"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO5552202.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(3904..6039)
                     /gene="igaA"
                     /locus_tag="ACHJ8C_002358"
     CDS             complement(3904..6039)
                     /gene="igaA"
                     /locus_tag="ACHJ8C_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO5552203.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTSEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQEIR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            6359..6919
                     /gene="nudE"
                     /locus_tag="ACHJ8C_002359"
     CDS             6359..6919
                     /gene="nudE"
                     /locus_tag="ACHJ8C_002359"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO5552204.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     APDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(7085..9637)
                     /gene="mrcA"
                     /locus_tag="ACHJ8C_002360"
     CDS             complement(7085..9637)
                     /gene="mrcA"
                     /locus_tag="ACHJ8C_002360"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO5552205.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVVLSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRHDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            9757..10536
                     /gene="hofM"
                     /locus_tag="ACHJ8C_002361"
     CDS             9757..10536
                     /gene="hofM"
                     /locus_tag="ACHJ8C_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO5552206.1"
                     /translation="MAFKNWQIGLHLQQQEAVAVAIVRSAKECLLHRWWRLPLENDII
                     KDGRIVDVQRLANTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQMAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPES
                     VALCGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKVY"
     gene            10536..11075
                     /gene="hofN"
                     /locus_tag="ACHJ8C_002362"
     CDS             10536..11075
                     /gene="hofN"
                     /locus_tag="ACHJ8C_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO5552207.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVNAVLLQAEQQLARSLQITKPRLLERQQLREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSIAALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            11059..11499
                     /gene="hofO"
                     /locus_tag="ACHJ8C_002363"
     CDS             11059..11499
                     /gene="hofO"
                     /locus_tag="ACHJ8C_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO5552208.1"
                     /translation="MNMFFDWWFATSLRLRQFCWAVWLLMLVTLIFLSSTHHEESDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVYWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            11489..11893
                     /gene="hofP"
                     /locus_tag="ACHJ8C_002364"
     CDS             11489..11893
                     /gene="hofP"
                     /locus_tag="ACHJ8C_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO5552209.1"
                     /translation="MRVKRWLLAGITLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPHWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            11805..13043
                     /gene="hofQ"
                     /locus_tag="ACHJ8C_002365"
     CDS             11805..13043
                     /gene="hofQ"
                     /locus_tag="ACHJ8C_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO5552210.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KAALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTA
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            13444..13965
                     /gene="aroK"
                     /locus_tag="ACHJ8C_002366"
     CDS             13444..13965
                     /gene="aroK"
                     /locus_tag="ACHJ8C_002366"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO5552211.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVEIPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            14022..15110
                     /gene="aroB"
                     /locus_tag="ACHJ8C_002367"
     CDS             14022..15110
                     /gene="aroB"
                     /locus_tag="ACHJ8C_002367"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO5552212.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVILVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            15202..16488
                     /gene="damX"
                     /locus_tag="ACHJ8C_002368"
     CDS             15202..16488
                     /gene="damX"
                     /locus_tag="ACHJ8C_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO5552213.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKARNEEPELEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            16595..17431
                     /gene="dam"
                     /locus_tag="ACHJ8C_002369"
     CDS             16595..17431
                     /gene="dam"
                     /locus_tag="ACHJ8C_002369"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO5552214.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            17449..18126
                     /gene="rpe"
                     /locus_tag="ACHJ8C_002370"
     CDS             17449..18126
                     /gene="rpe"
                     /locus_tag="ACHJ8C_002370"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO5552215.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            18119..18877
                     /gene="gph"
                     /locus_tag="ACHJ8C_002371"
     CDS             18119..18877
                     /gene="gph"
                     /locus_tag="ACHJ8C_002371"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO5552216.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADILMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            18870..19874
                     /gene="trpS"
                     /locus_tag="ACHJ8C_002372"
     CDS             18870..19874
                     /gene="trpS"
                     /locus_tag="ACHJ8C_002372"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO5552217.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            20034..20939
                     /gene="yhfZ"
                     /locus_tag="ACHJ8C_002373"
     CDS             20034..20939
                     /gene="yhfZ"
                     /locus_tag="ACHJ8C_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO5552218.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLTHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            20956..21318
                     /gene="yhfY"
                     /locus_tag="ACHJ8C_002374"
     CDS             20956..21318
                     /gene="yhfY"
                     /locus_tag="ACHJ8C_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO5552219.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            21402..22565
                     /gene="yhfX"
                     /locus_tag="ACHJ8C_002375"
     CDS             21402..22565
                     /gene="yhfX"
                     /locus_tag="ACHJ8C_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO5552220.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVSDAVEQGTDVITVFTLDKAREISAAAVKTGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIQNLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGVTHTEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQRITETYLNAVDDSSIDYTL
                     PLAGEHPVSSAVVLCFRTQIFITRSDVVLVSGIHRGEPEIVGRYDSLGNPLEA"
     gene            22566..23792
                     /gene="yhfW"
                     /locus_tag="ACHJ8C_002376"
     CDS             22566..23792
                     /gene="yhfW"
                     /locus_tag="ACHJ8C_002376"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO5552221.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLETLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKMGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLNEFNTYPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSFRFAGDTL"
     gene            23789..24667
                     /gene="php"
                     /locus_tag="ACHJ8C_002377"
     CDS             23789..24667
                     /gene="php"
                     /locus_tag="ACHJ8C_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO5552222.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     ARGVRNVIEMTNRYMGRNAQFMLDVMHETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGLIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDFLLATFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            24678..25031
                     /gene="yhfU"
                     /locus_tag="ACHJ8C_002378"
     CDS             24678..25031
                     /gene="yhfU"
                     /locus_tag="ACHJ8C_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO5552223.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFDVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            25043..26347
                     /gene="yhfT"
                     /locus_tag="ACHJ8C_002379"
     CDS             25043..26347
                     /gene="yhfT"
                     /locus_tag="ACHJ8C_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFO5552224.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGILILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGMSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFLPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            26359..27444
                     /gene="yhfS"
                     /locus_tag="ACHJ8C_002380"
     CDS             26359..27444
                     /gene="yhfS"
                     /locus_tag="ACHJ8C_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO5552225.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTCGDLGLTP
                     GLNQPRITQRVEQVLADAFHAPAAALVQGAGTGAIRAALAALLKSGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQIIEAQQPDAALVQHTRQQPQDRYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAQALEVLRGLVLAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKLGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(27551..>27716)
                     /gene="yhfL"
                     /locus_tag="ACHJ8C_002381"
     CDS             complement(27551..>27716)
                     /gene="yhfL"
                     /locus_tag="ACHJ8C_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO5552226.1"
                     /translation="NKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISK
                     IIGGCGPTAQ"
BASE COUNT         6464 a         6874 c         7659 g         6719 t
ORIGIN      
        1 ctgaaaaaag caaaatacta ctgcccggat gcgataatta acccaccttt tgattctgga
       61 taaacaacat ggataacgtt gaactttcac cggcaacacg ctgggggatg attgctaccg
      121 gattacttca ggggttggtt tgctacctgc tgatcgcctg gcttgccgga aaaaatctca
      181 gttggattgt ttatggcgtg cctgcgaccg tcgcgttttc atccgtttta ctcttctccg
      241 tgatctcctt taaacagaag cgcctttggg gatggctggc gctggtgttt attgccacgc
      301 tgggaatgag cggctggctg aagtggcaaa ctgatggcat gaccccctgg agagttgaaa
      361 aggcgctttg ggattttggc tactatctgc tgctgatggc aatgttgttg ctaccgtgga
      421 tacaacaaag ccttcgcatc cgcaatgaca gtagccgcta tcgctacttt taccagtctg
      481 tatggcataa cgtacttata ttactggtga tttttctcgc caatggcctg acgtggctgg
      541 tgcttttact gtggagtgag ttgtttaaac ttgttggcat cacgtttttc aaaacgctct
      601 tttttgcaac cgactggttt atttatctca cgttaggtct ggctaccgcg ctggcggtga
      661 tcctcgcgcg aacacagtca cgtttaatcg actctattca aaagttgttc acgttaatcg
      721 ccacggggtt gctgccgtta gtatcattgc taaccctgat gtttattatc accctgccgt
      781 ttacgggcct gagcgcgatt tctcgccaca tctccgccgc tgggttgctg ttaacgctgg
      841 cctttttgca attgatctta atggctattg tccgcgatcc gcaaaaagcg tcacttccct
      901 ggacggggcc attgcgttgc ctgattaaaa ccgctttgct ggttgctccg ctgtatgtgt
      961 tcgtcgccgc ctgggcgtta tggctgcggg tcgctcagta cggctggact gccgaccgct
     1021 tgcagggcgt gctggcggtg ctggtgttac tggtatggtc gctggggtat tttgtcagta
     1081 tcgtctggcg taaagggcaa aatccccttg atcttcaggg caaagtgaac cttgcggttt
     1141 cgttattggt gttggtaata ctggtgcttc ttaattcgcc ggtgctggac agtatgcgca
     1201 ttagcgtgaa cagtcatatg gcgcgttatc agagcggcaa aaacacgcca gaccaggtaa
     1261 ctatctacat gctcgaacag agcggtcgct atggacgtgc ggcgcttgag tcgctgaaaa
     1321 gcgatgccga gtatatgaaa gacccgaaac gcgcgcggga tctgctgatg gcgttagatg
     1381 gagagcaaca tcttcaggaa caggtatcgg aaaaagtatt agccgataat gtgttaattg
     1441 ccccaggttc tggtaaaccg gatgcgacat tctggtcggc cttaatccag gatcgctata
     1501 acgtgatgac ctgtattgaa aaagacgcct gcgtgctggt agagcaagat ctgaatagtg
     1561 atggcagggc agagcggatc ctgtttgctt ttaatgatga tagagtcatt gtctatggct
     1621 ttgactcagc cagaaaagaa tgggacgcgc ttgatatgag tttacttccg cgccaaataa
     1681 cgaaagaaaa attactcgct gctgcgaagg atggaaagct ggggacgagg cctaaagcgt
     1741 ggcgcgatct tgtagtggat ggtgaaaggc tggatgtgaa tctgaatgag tgacaaagcg
     1801 catcaggtgt attagtcgta aaatccggca gagtcctctc tgccggacat actcattaat
     1861 gaacttgcgg atctgccgga gacgcgttgt tgcggatctc ggcaatatcc atcgcattga
     1921 acagatagtg gttaccgcag taatcacaat gcatgtcaat ttcgccatct tccgccagga
     1981 tgctatcgac ttcttcatca ggcagcgttt tcagcgcatc ggcgcaacgt tcacgcgagc
     2041 aggtgcattt gaactccaca tcctgcggat cgtaaaccgt cacctcttct tcgtgataca
     2101 aacgccacaa cacttcgttt gccggtaaag tcagcagttc ttcggttttg atggtttcgg
     2161 ttagcgttgc caggtggtca aagtcgtcct gctgggcatt ttgcgcaggc attacctgca
     2221 acaacattcc gcctgcagcc ggtttgccgt ctacgtcgcc ggtgcgaata aacaggcgcg
     2281 tcggcagctg ttcagaacgc ataaagtaat cttccaggca ggccgccagg gtatcacctt
     2341 ccagaccaac cacgccctga tagcgttcgc cttcgctcgg ggtgatggtg atcaccacgt
     2401 aaccattgcc gaccagcgtt ttcaggtcgg cattttctgg aatttcgccc tgcacgcgcg
     2461 ccacaccgcg catctgctgg ttgttgttac cgttaataac cgccagattc atcggaccgt
     2521 cgccttgcag ctgtacggtg atatcaccat caaacttcaa cgtagcggtt aacaggctgg
     2581 tcgcaaccag cagttctgcc agcacgttct taacgggctg cggataatcg tgattctcaa
     2641 ggatctgttg cagggtttcc gaaacggtta ccagttcgcc gcgaacggca aagttttcaa
     2701 acagatagcg atgtaattgg tcatgttgcg gcataatcat ctctcttgca ggtgacagtt
     2761 attcactgtc gccgtgttta aatcgtaaca ggtcgcggcg ctcttttttg tccgggcgtc
     2821 ggtccggatg cggcatggtt aaggcattaa gtttacgtgc cagcgccatt ttttcgcgtt
     2881 tctctacact ttccgcagtc tcttcataca gcaaggctgc ctcgctggcg gggcgacgct
     2941 gttcagtaat cgcctttaca atcaccgtgc gttcgtcatt tccctggcgc agagtgagcg
     3001 tggcattcag ctcgacgatt ttgcttggct tgctgcgctg cccgttgtaa tgcaccttac
     3061 cgccttcaat catttcacgg gccagcgcgc gggttttata aaaacgggca gcccatagcc
     3121 atttatccag tcgaacctca acagcaggtt tttctttcat ggcatctcct tcacattagc
     3181 gaggggatca ggcggcggta gtcattcagt gacggatggc gttgatactg tttctcggca
     3241 atcccggagt caggattagt cacaccgagg cagtaacgaa taccaaattg cgcggcagca
     3301 tcgagaatcg cttcgctgtc atcaataaac agcgttcttt cagctttaag acccgtagct
     3361 tcggccaccg catgccataa ccgctgatcc tctttcggat aaccaaatgt gtgggtggaa
     3421 agtaataaat caaggtgtgc gtccagaccg gtatgctcaa gttttaccgc caggttgtgc
     3481 ggatgcgcat tggtgagcaa aattcgctgt ttaccgctgg ctttcagtgc ctcaagaaac
     3541 ggaatggtat cttcacgcag tacggcacgc ggtcccatct cggtggtcat cgcacagata
     3601 tccagaccca gttgctcact ccagtaatca agacagtacc agtttagcgt atgctgtacg
     3661 tcgtgatatt gctggcgcat atattccatc gcttcctgtg gcgtaactcc gtttttcgcg
     3721 ccccatgttt caggcaccag cttttgccag aaatagttat cgaaggcgag gtcgagcaac
     3781 gtgccgtcca tatccagcag aacggtatct acgtcctgcc aggcaatgtt gatatgcatg
     3841 agggaaatct ccagagtgaa gcaatttgcg cgacagggta gcataacctg ccgcgcaaac
     3901 gtgttattcg ataaggcttt ctgaaggggt gatcagctgc gggttcaggc agctttcata
     3961 ataggcctga atcttcatca tgcgagtacg atgacgctgg taacgacgcc aggcctgtac
     4021 gccattataa atggcgctac ccagcatcgt cagtagcagc agcgtagtgc tgagatagcg
     4081 ccacaggccg gaacgatccg ggatcggatg aaggccaata tgctgcgtac cattggcgtc
     4141 agtgaagatt tttgtgacga taccttcggc gttaaacggc gtatgcatca gcatttgtgc
     4201 cagtttctgg aaagcgttcc actgttcttg cggcggatag tcgtaaagtg atgccgaagg
     4261 ccagggctga tcaacaaaat cgctgccttc gtcgctgaca atcaggaacc cgccgggggc
     4321 cggactgttt agtgattgtg ccgctcgcgc cgtttcatgc gtgataaacg gcgcggtaga
     4381 ggtggccacc aggttatcca gcgattccgc actcaccggg cgtaataaca cattcacgcc
     4441 atcgagtttc ccggcgttgg cgcgttttac cagcgcgtcc cagtctttac tgttgccgag
     4501 attgaccagc gcatttttca gtcgcacaca gtcatctttg gcagaacaca gatctgctgt
     4561 cttcagtaca atgtcgccaa aatcatcaag caataccata ccggattttt gaattgctga
     4621 acgtaatgag gcactgacgc gagattcatc ttccggttta gggtgcagct ggcgattaac
     4681 tgcttcggtc aatgccgtcg ctttgttgac cagttcagat tctggtaatg gcaatgagcg
     4741 ggcgtcgttc cagatgatct gcgagcagtc aaacggtaaa aaaggtgaat tggtttttgc
     4801 gctccaggtt ccggaagtgc gaatattaca cattcccgta ccgctaatac gcagtgtatc
     4861 gccaacccgc acgccagcgt cagccagttg ttttacgctg gtggcttcaa tggtctgtgc
     4921 gcctttcatc cacgagaggg tgaatttcag cggcatatcc agcgggatcc agaataacag
     4981 cataaacagc accaatagcg agcccgcagc aataatcgta ctgcgcagcc agtgctgtaa
     5041 cggaaagttt tttacttcat catgcagcga aagatatcgc ccctgacgca ctacgtggcg
     5101 gtcgagatag atatcgatat cggtttgttg accaagatcc tgagcaatgt atggctgcca
     5161 gtgtgcggga tagaccaggt cgataatacc gagtgaaata ttgttgatct gttcctgatc
     5221 gttttcgcca aacaatcccc aacgacgggg tgtaccgcgc agacaatgaa tttcccgcag
     5281 ggaggatttt gccgggggcg cgaatagccc ccacagacct gcgcccagca gcagtaacgc
     5341 gccgcctgcc agccacggaa caaagacatc cggggtaatc aggcagaaaa agaacatcag
     5401 gaaggaggcg acaatcagca acgcttcgcg cagcccgcgc ggacgactca gcgcatactc
     5461 ttcgtgcgtt tctttgcgaa tattgagcag ttctatttgc tcgctctctt ccccgcgaat
     5521 cgacgcctgc gttgacggaa caggttgcaa ggcatagccg cgaatttcct gcatgtactc
     5581 ctgcagcgta tgaccgttga gtgaaataac cagcggcagc gaatcggtat gaatcagttc
     5641 aacggtattc tcatcgttga tgtactgttc ccagaaaggc ggcaggtgga cttctaccga
     5701 atcgaggtag taacgccatt tattcggatc gtcggtagag atgccataac gcgtgatagc
     5761 gtgtgtcagc atcatgacgt tgttgctttc ggcattcagc gccagagaga tcggtgccgc
     5821 gctggctccc gttgggccag gcacctgtaa tacctgcgtc aggctctcaa gataattttc
     5881 aacggcgcta cgttcttcag atgtgagttt acgcgtttgc gcatccgcga aggcgttggt
     5941 ccagggcagc tgacgccgtc tggatcgcac ttttatcagc catcccgcaa gtagtgagca
     6001 ggccagcaaa gcagctaaaa aaatcacaat ggtgctcatg ctttccccat cttacttagt
     6061 ctgtcaggcg tggtcagtcg caccggacac aatacatgat tctgcggttg gctatcggca
     6121 agcgtggagt ttaacttgag catcattaag cacatcacag taaccgctga tcatagcaaa
     6181 ccctctgaag gcggaatatc agcaaattcc tggagttatt tcaacctatt gacttttaat
     6241 ttgatttgag gacgattatt tattaagtta cataaatgta aataacgcgc aattcacatt
     6301 gccgaaagcg tgtccgatat cgcacaataa ccgctgattt cacagcatag atcccacgat
     6361 gagcaaatca ttacaaaaac ccaccattct gaatgttgaa actgtagccc gttcccgact
     6421 gtttaccgtc gaaagcgtgg atctggagtt cagcaatggc gtgcggcgtg tttatgaacg
     6481 aatgcgtcca accaaccggg aagcagtgat gattgtgccg attgtggacg atcacctgat
     6541 cctgatccgc gaatacgcag tgggaactga atcctacgaa ttaggttttt cgaaaggatt
     6601 aattgatccg ggtgaaagcg tctttgaagc cgctaaccgc gagctaaaag aagaggttgg
     6661 atttggtgcg aacgatctga cttttttgaa gaagctcagc atggcaccgt cttacttttc
     6721 cagcaaaatg aatatcgtgg tagcgcaaga tctctacccg gaatcactgg aaggcgatga
     6781 gccagagccg ttaccacagg tgcgctggcc gctggcgcat atgatggatt tgctggaagc
     6841 ccctgacttc aacgaagcgc gtaatgtcag cgcgctgttc ctcgtgcgcg aatggttgaa
     6901 agggcagggg cgagtgtaaa tgcgtgaagt ttgcctgatg cacaacgctt atcaggctta
     6961 cgaagttcct gcaatttatt gaattttcaa ttttttgttg gccggataac gcgttcaccc
     7021 gcatccggca taaacaagtg cactttgtca gcaaactgaa aaaggcgccg aagcgccttt
     7081 ttaatcagaa caattcctgt gcctcgccat tatcgataat ggtcgttccc acctcgtgca
     7141 ccgcctgttg tgtcggctgc gtaccttcga tgaaatactc ttcgcggctg ttgccaccat
     7201 tagctaattg cccggtgctg cgatcgatat tcaccgtcac aatacccggt ggcggcgtca
     7261 gtggctgctc cggcacacct tcaagaacgg ctttcatata agcgtcccat gcaggctggg
     7321 cactcttggc accgccttcg taacctgaga tctgatcttt aatcgctccg gaagccgttg
     7381 tatgaccgag attacgacgg tgatcatcaa agccaatcca gaccgaggtc acaacgcccg
     7441 gaccgtaacc cgagaaccac gcatctttcg aactgttagt ggtacccgtt tttccgccta
     7501 tatcatgccg ttgtaaatca cgacctgctc gccagccagt accctgccag cctggttcac
     7561 caaaaatatt ggtgttcaag gcactcttaa tcaggaatgc cagcggtgtg ttgatgacgt
     7621 gcggtgcgta ctcctgcgcg ccggtcttcg ccactaacgc ctgatttgcc tgctccagct
     7681 gcggcattgg tacagaaaca ttctgctgtt cgcgggagat agcgacatct tcaacatcgt
     7741 tattttccag cacgttcgat ttctgcgtat caccgtaaat caccggaata tcgcattccg
     7801 ggcaggctac tttcggtttc gcttcgaaaa tcacgccgcc ctgatcgttt tcaattttgc
     7861 tgataaacca tgggtccacc aggaagccgc cgttcgccat gaccgcgtag ccgcgcgcca
     7921 cctgcattgg ggtgaaggaa gctgaaccca gcgccagcga ttcggtgtgg acaatgtttt
     7981 gtgccgggaa gccgaagcgt tgcagatatt ctgcagcgta gtcgacgccc atcgcccgca
     8041 ttgcgcgtac catcaccacg tttttcgact gacccagtcc ctgacgtaag cgaattggac
     8101 cggcatactg cggtggtgag ttcttcggct gccagtcaga accggcacct gcatcccagc
     8161 gggaaattgg cacatcgttc aacatacttg ccagcgtcag acctttatcc atcgccgcgg
     8221 tgtagaggaa cggtttgatg ttggaaccca cctgacgcag tgcctgggtg gcgcggttaa
     8281 acttgctctg attgaaatca aagccaccga ccagcgccat aacggcaccg ttttgcggat
     8341 tgatcgacac cagcgccgag ttcacttccg gcacttgcgc cagccaccat gcatcgccaa
     8401 cttgacgaac ccagatttgc tgacccgttt gcagaacatc ggtcacttta cgtggcgtcg
     8461 gtccctgctg agtatccgaa cggtaaggac gcgcccagcg aacaccttcc atactcaata
     8521 cgacggtcga cccgtctgcc agcatcgccg ttgcttcctg aggattggcg ctggtgactg
     8581 cggcaggcag cagcggacca taggtcggca gcgccttcag cgtatcggta attttgttgt
     8641 tgtcccacgc cgattcgccc actttccaca gcacatttgc cgggccgcga tagccgtggc
     8701 gcatgtcgta gtccagcacg ttattacgta ccgcctgctg tgcggcctgc tgcactttgc
     8761 gggtgatggt ggtgtaaatg cgataaccgt cttcataggc actttcgcca taacggttat
     8821 acatctcctg gcgcaccatt tcgctcaggt atggcgcaga gaaagcaatc tccggcgcgt
     8881 gatagttagc gttaatcgcc tcagtgcgtg tctgatcgaa ctgttgttgg gtgatatacc
     8941 cttcatccag catccgcgac agcacgacgt tacgccgcgc gacggcacga tccattgagt
     9001 agagcgggtt gaaggtggaa ggcgctttcg gcagcccggc tatcaccgcc atttcgttca
     9061 acgtcagttg gtcgacagtt ttaccgaaat agacttgtgc cgcagcaccg acaccatagg
     9121 cgcggtaacc gaggtaaatt ttgttcagat aaagctcgag gatctcgtct ttcgtcagca
     9181 actgttcaat gcgaatcgcg aggaagactt ccttaatctt gcgcatcagc gtgcgttctg
     9241 gactgaggaa gaagtttctc gccagctgct gggtaatggt actcgcccct tgtgacgcgt
     9301 gaccggagaa cagcgccacg cttgccgcac ggaagatccc caccgggtca acgccgtgat
     9361 gctcgtagaa gcggctgtct tccgtcgcga taaaggcttt caccatctcc ggcgggattt
     9421 gatccaacgt aaccggaata cgacgtttct caccgtattg agcaatcagc tcgccatcgg
     9481 cgctgtaaat ctgcatcgga atttgcaggc gaacatcttt taatgtcgcc acatccggca
     9541 gttgtggctc gatgtagcgg tataggccat aaatcgagcc tgctcccagc agaatgcaac
     9601 agactgcaag gatcaaaaaa tactttacga acttcactgg aaatttccca tttagtttca
     9661 tttgggcagt ttataaacaa acgcgcggta gtataaaggc aagccagacg cattgatata
     9721 cccgtcagag tgacgggtga taaggagatc atcacaatgg catttaagaa ctggcaaatt
     9781 ggtttgcatt tacaacagca agaagcggta gcggttgcta tcgtgcggag cgcaaaagaa
     9841 tgcttattgc accgctggtg gcggttgcca ctggagaacg acattatcaa agatgggcgg
     9901 attgttgatg tgcagcggct ggctaacacg ttgttaccgt ggagtcgtga actgccgcag
     9961 cgtcatcaca ttatgttggc gtttcccgcc agtcgcacat tacagcggtc atttccacgc
    10021 ccgtcgatgt ccctcggtga gcgggagcaa atggcctggc tgtccgggac gatggcccgt
    10081 gaactggata tggatccgga ctccctgcgc ttcgattaca gcgaagactc actcagtccc
    10141 gcctataacg tgactgccgc gcaaagcaaa gagctggcaa cgctgcttac tctggcagaa
    10201 aggttgcgtg ttcatgttag cgcgatcact ccggatgcca gtgcattaca gcgattcctg
    10261 ccttttttac cttctcatca gcaatgtctg gcctggcgtg ataacgaaca gtggctgtgg
    10321 gcgacgcgct atagctgggg gcgcaaactg gcggtgggga tgactagcgc gaaggagctg
    10381 gcggcagcgt tatctgttga tcccgaaagc gtcgcgctat gtggcgaagg cggatttgac
    10441 ccctgggagg cggtttctgt tcgtcagccg ccgctaccgc caccaggtgg agactttgcc
    10501 atcgcgctgg ggctggcgct tgggaaggtg tactgatgaa cccgccaatt aattttttgc
    10561 cctggcgaca gcaacgccgg actgcttttc ttcgtttctg gttgctgatg ttcgttgcgc
    10621 ctctgctgct ggccgtcggg ataacgctaa tactgcgtct gacaagcaat gccgaagcac
    10681 gcgtaaacgc cgttttgctt caggcggaac aacaactcgc ccgcagctta cagataacga
    10741 aaccacgttt gctggaacgc caacagttac gcgaacagcg tttgcaaagg cagcgccagc
    10801 gacaatttac ccgcgactgg caatctgcgc tggaagcact ggcggcgctt ttacccgaac
    10861 atgcctggct gacaacgata agctggcagc agggaacgct ggagatcaag gggctgacaa
    10921 caagcattgc cgcgttaaac gcgctggaaa cgtcactccg ccaggatgct tcttttcatc
    10981 tcaatcagag gggggccacg cagcaggatg cgcagggacg ctggcaattt gagtatcagt
    11041 taacaaggaa ggttagcgat gaacatgttc tttgactggt ggttcgccac atcactccgc
    11101 ctccgccagt tttgctgggc agtctggttg ctgatgttag ttacgctcat ttttctgtca
    11161 tcgacacacc atgaagagag cgacgcatta attcgactac gggcaagtca tcaccagcag
    11221 tgggccgcac tgtatcgcct ggttgacacc actcccttca gcgaggaaaa aacgctgccc
    11281 ttttcgccac tggattttca gttatccggc gcgcaactgg tttactggca tccatccgcg
    11341 cagggaggtg agttggcgtt gaaaacgctg tgggaagcag tgccgtcggc atttacacgg
    11401 ctggcagagc gcaacgtcag cgtgagccgt ttttcgttaa gcgtggaagg tgatgacctt
    11461 ttgttcacgc tacaactgga gacgccgcat gagggttaaa cgctggttgt tggcaggtat
    11521 tacattgtgc cttttaaccg gtatgcgtga cccttttaaa ccgccggaag atctatgccg
    11581 gattagcgaa cttagccagt ggcgctatca ggggatggta gggcgaggtg agcgcatcat
    11641 cggtgtaata aaagacgggc aaaaaaaatg gcgacgggtg cagcaaaacg acgtgctgga
    11701 aaacggctgg acaattttac agctgacgcc agacgcacta acgctgggaa ccgggacaaa
    11761 ctgcgaaccg ccacactggt tgtggcaacg gcaaggagat acaaatgaag caatggatag
    11821 ccgcactact gttgatgctg atacccggcg tacaggcggc aaagccgcaa aaagtgacgc
    11881 tgatggtgga tgacgttccg gtggctcagg tgttgcaggc gctggctgaa caggagaagt
    11941 tgaacctggt ggtttcgcca gacgtcagcg gtacggtgtc gttacattta actgacgttc
    12001 cctggaagca ggcactacaa actgtagtga aaagcgccgg attgataacg cgccaggagg
    12061 gcaacattct ctcggtgcat tccgttgcct ggcagaatga caatatcgcc cgccaggagg
    12121 cggagcagac gcgggcgcag gcaaatctgc cgctggaaaa tcgcaatatt actctgcaat
    12181 acgccgacgc cggagagctg gcgaaagcgg gggagaagct actgagtgcc aaagggagta
    12241 tgaccgtcga taaacgcacc aatcgccttt tgctgcgaga taacaaagcg gcgttaagcg
    12301 cgcttgaaca gtgggtagcg caaatggatc tgccggtcgg gcaggttgag ctgtcggcgc
    12361 atattgtcac cattaatgaa aaaagtttgc gtgagttagg tgtgaaatgg acgctggctg
    12421 atgcgcaaca cgctggtggc gttgggcaag tcaccgcgct tggtagcgac ctctccgtag
    12481 cgacggcgac aacgcatgtc ggttttaaca ttggacgcat caacggacgt ttactggatc
    12541 ttgagctttc cgcgctcgaa caaaaacagc agttggatat tatcgccagt ccgcgtctgc
    12601 tggcctctca tcttcagcct gccagcatta aacaggggag cgaaattcca tatcaggttt
    12661 ccagcgggga aagtggcgcg acgtcggtgg aatttaaaga ggccgtcctg gggatggagg
    12721 tcacgcccac ggtgttacaa aaaggtcgca tccggctgaa attacacatc agccagaacg
    12781 ttccggggca ggtgctacag caggcagatg gcgaagtgct ggcgattgat aagcaggaga
    12841 tcgaaacgca ggtcgaggtc aaaagcggag aaacgttggc gctgggcggc atttttaccc
    12901 gtaaaaataa atcgggtcag gatagcgtac cgttgcttgg cgacattccc tggttcgggc
    12961 aattatttcg tcatgacgga aaagaagatg aacgacgcga gttagtggtg tttatcacgc
    13021 cacgactggt ttccagtgag taaacagccg taaaagcggt aatgttttta cgctgaacgt
    13081 gtttcatcta tttgacgcgc gcaggtattt agcatacaag gagtaccgat ttgagagttg
    13141 gtgctcttcg ctgcctgcgt tccatgatga tgatttatca ttcaggcggc attttgctgt
    13201 cttttttacg ctaatcttac ccggtgattt atcgccagag cggtggtagc aaggcagcgc
    13261 gcttgcagcg accagatatg cagagggatg ggtgatttat tcagttgcca aacccgctgg
    13321 agtattgaga taattttcag tctgactctc gcaatatctt atgaggtttc agttcatgtc
    13381 ctgcggcgct ctctgagcga agcgggttta tcattaacga atagtcttag tagtaccgaa
    13441 aaaatggcag agaaacgcaa tatctttctg gttgggccta tgggtgccgg aaaaagcact
    13501 attgggcgcc agttagctca acaactcaat atggaatttt acgattccga tcaagagatt
    13561 gagaaacgaa ccggagctga tgtgggctgg gttttcgatt tagaaggcga agaaggcttc
    13621 cgcgatcgcg aagaaaaggt catcaatgag ttgaccgaga aacagggtat tgtgctggct
    13681 actggcggcg gctctgtgaa atcccgtgaa acgcgtaacc gtctttccgc tcgtggcgtt
    13741 gtcgtttatc ttgaaacgac catcgaaaag caacttgcac gcacgcagcg tgataaaaaa
    13801 cgcccgttgc tgcacgttga aataccgccg cgtgaagttc tggaagcgtt ggccaatgaa
    13861 cgcaatccgc tgtatgaaga gattgccgac gtgaccattc gtactgatga tcaaagcgct
    13921 aaagtggttg caaaccagat tattcacatg ctggaaagca actaattctg gctttatata
    13981 cactcgtctg cgggtacagt aattaaggtg gatgtcgcgt tatggagagg attgtcgtta
    14041 ctctcgggga acgtagttac ccaattacca tcgcatctgg tttgtttaat gaaccagctt
    14101 cattcttacc gctgaaatcg ggcgagcagg tcatattggt caccaacgaa accctggctc
    14161 ctctgtatct cgataaggtc cgcggcgtac ttgaacaggc gggtgttaac gtcgatagcg
    14221 ttatccttcc tgacggcgag cagtataaaa gcctggctgt actcgatacc gtctttacgg
    14281 cattgttaca aaagccgcat ggtcgcgata ctactctggt ggcgcttggc ggcggcgtag
    14341 tgggtgatct gaccggcttc gcggcggcga gttatcagcg cggtgtacgt ttcattcaag
    14401 tcccgacgac gttactgtcg caggtcgatt cctccgttgg cggcaaaact gcggtcaacc
    14461 atcccctcgg taaaaacatg attggcgcgt tctaccagcc cgcttcagtg gtggtggatc
    14521 tcgactgtct gaaaacgctt cccccgcgtg agttagcgtc ggggctggct gaagtcatca
    14581 aatacggcat tattcttgac ggtgcgtttt ttaactggct ggaagagaat ctggatgcgt
    14641 tgttgcgtct ggacggtccg gcaatggcgt actgtattcg ccgttgttgt gagctgaaag
    14701 cagaagttgt cgccgccgac gagcgcgaaa ccgggttacg tgctttactg aatctgggac
    14761 acacctttgg tcatgccatt gaagctgaaa tggggtatgg caattggtta catggtgaag
    14821 cggtcgctgc gggtatggtg atggcggcgc ggacgtcgga acgtctcggg cagtttagtt
    14881 ctgccgaaac gcagcgtatt ataaccctgc tcacgcgggc tgggttaccg gtcaatgggc
    14941 cgcgcgaaat gtccgcgcag gcgtatttac cgcatatgct gcgtgacaag aaagtccttg
    15001 cgggagagat gcgcttaatt cttccgttgg caattggtaa gagtgaagtt cgcagcggcg
    15061 tttcgcacga gcttgttctt aacgccattg ccgattgtca atcagcgtaa caacaagaaa
    15121 ggtcaggccg cttatcaagc ggtctattag cttcaggtta attgcaacgt ggtaagcatt
    15181 aaccttttag tggggtgtta aatggatgaa ttcaaaccag aagacgagct gaaacccgat
    15241 cccagcgatc gtcgtactgg tcgttctcgt caatcttctg aacgttctga gcgtactgaa
    15301 cgtggcgaac cgcagatcaa ttttgatgat attgaacttg atgacactga cgatcgccgt
    15361 ccgactcgtg cgcaaaaagc gcgtaatgag gaaccggaac tcgaagaaga aattgacgaa
    15421 tccgaagatg aaaccgtgga tgaagagcgc gtagagcgtc gtccacgtaa gcgcaaaaaa
    15481 gcagccagta aacccgcttc tcgtcagtat atgatgatgg gcgtcggcat tctggttctc
    15541 ctgctgttga tcatcggtat cggttctgcg ctaaaagccc cctcgacctc ttccagcgat
    15601 caaaccgcgt ctggcgagaa gagtattgat cttgctggca atgcgaccga tcaggcgaat
    15661 ggcgtgcagc cagcgccggg aaccacgtct gcggaaaata ctcagcagga tgtttctcta
    15721 ccgccgatct cttctacgcc gactcaaggg caaaccccgg cggcaacgga tggtcaacaa
    15781 cgtgttgaag tgcagggtga cctgaacaat gcgctgaccc agccacaaaa tcagcaacag
    15841 ttgaataatg tggcggtcaa ttccacgttg ccgactgaac ccgcgacggt cgcgcctgtt
    15901 cgcaatggca atgcatcgcg tgacacggcg aaaacgcaaa ccgctgaacg tccggccact
    15961 acgcgtccag ctcgccagca ggcggtgatt gaaccgaaaa aaccgcaagc aaccgtgaaa
    16021 acggagccga agccggtagc acagacgccg aagcgtactg aaccagctgc ccctgtggca
    16081 agcacgaagg caccggctgc gacttctacg ccagcaccaa aagagacggc gactacggct
    16141 ccagtacaga cggcatcccc ggcgcaaacc acggcaacac cagccgctgg agggaagacc
    16201 gcaggtaatg ttggttcgtt gaaatcggca ccgtccagcc attacactct gcagctgagc
    16261 agttcctcta actacgacaa cctgaacggt tgggcgaaga aagagaatct gaaaaactac
    16321 gttgtctatg aaacgacgcg taatggtcag ccgtggtatg tcctggtttc tggcgtgtac
    16381 gcttcgaaag aagaggcgaa aaaagcggta tctacattgc cagcagatgt ccaggccaaa
    16441 aacccgtggg cgaaaccgct gcgtcaggta caggccgatc tgaagtaatc aaggttatct
    16501 cccgcaatgg tttatcattg cgggcgttgc ctgaagcgct ggatgctgtc ggagctttct
    16561 ccacagccgg agaaggtgta attagttagt cagcatgaag aaaaatcgcg cttttttgaa
    16621 gtgggcaggg ggcaagtatc ccctgcttga tgatattaaa cggcatttgc ccaagggcga
    16681 atgtctggtt gagccttttg taggtgccgg gtcggtgttt ctcaacaccg acttttctcg
    16741 ttatatcctt gccgatatca atagcgacct gatcagtctc tataacattg tgaagatgcg
    16801 tactgatgag tacgtacagg ccgcacgcga gctgtttgtt cccgaaacaa attgcgccga
    16861 ggtttactat cagttccgcg aagagttcaa caaaagccag gatccgttcc gtcgggcggt
    16921 actgttttta tatttgaacc gctacggtta caacggcctg tgtcgttaca atctgcgcgg
    16981 tgagtttaac gtgccgtttg gccgctacaa aaaaccctat ttcccggaag cagagttgta
    17041 tcacttcgct gaaaaagcgc agaatgcctt tttctattgt gagtcttacg ccgatagcat
    17101 ggcgcgcgca gatgatgcat ctgtcgtcta ttgcgatccg ccttatgcgc cgctgtctgc
    17161 gactgccaac tttacggcgt atcacacaaa cagttttacg cttgaacaac aagcgcatct
    17221 ggcggagatc gctgaaggtc tggttgatcg ccatattccg gtgctgatct ccaatcacga
    17281 tacgatgtta acgcgtgagt ggtatcagcg cgcaaaattg catgtcgtca aagttcgacg
    17341 cagtataagc agcaacggcg gcacacgtaa aaaggtggac gaactgctgg ctttgtacaa
    17401 accaggagtc gtttcacccg cgaaaaaata attctcaagg agaagcggat gaaacagtat
    17461 ttgattgccc cctcaattct gtcggctgat tttgcccgcc tgggtgaaga taccgcaaaa
    17521 gccctggcag ctggcgctga tgtcgtgcat tttgacgtca tggataacca ctatgttccc
    17581 aatctgacga ttgggccaat ggtgctgaaa tccttgcgta actacggcat taccgcccct
    17641 atcgacgtgc atctgatggt gaaacccgtc gatcgcattg tgcctgattt cgctgccgct
    17701 ggtgccagca tcattacctt tcatccagaa gcctccgagc atgttgaccg cacgctgcaa
    17761 ctgattaaag aaaatggctg taaagcgggt ctggtattta acccggcgac acctctgagc
    17821 tatctggatt acgtgatgga taagctggat gtgatcctgc tgatgtccgt caatcctggt
    17881 ttcggcggtc agtctttcat tcctcaaaca ctggataaac tgcgcgaagt acgtcgtcgt
    17941 atcgacgagt ctggctttga cattcgactg gaagtggacg gtggcgtgaa ggtgaacaac
    18001 attggcgaaa tcgctgcggc gggcgcggat atgtttgttg ctggttcggc aatcttcgac
    18061 cagccagact acaaaaaagt cattgatgaa atgcgcagtg aactggcaaa ggtaagtcat
    18121 gaataagttt gaagatattc gcggcgtcgc ttttgatctc gacggtacgc tggttgacag
    18181 tgcacccggt cttgctgctg cggtagatat ggcgctgtat gcgctggagt tgcctgtcgc
    18241 aggtgaagaa cgcgttatta cctggattgg taacggcgca gatattctga tggagcgcgc
    18301 attgacctgg gcgcgtcagg aacgtgcgac tctgcgtaaa acaatgggta aaccgcccgt
    18361 tgatgacgac attccggcag aagaacaggt acgtatcctg cgtaaactgt tcgatcgcta
    18421 ctatggcgag gttgccgaag aggggacgtt tttgttcccg cacgttgccg atacgttggg
    18481 cgcgttgcag gctaaaggcc tgccgctagg cctggtcacc aacaaaccga cgccgttcgt
    18541 cgcgccgctg ctcgaagcct tagatatcgc caaatacttc agcgtggtga ttggtggtga
    18601 tgatgtgcaa aataaaaaac cgcacccgga cccgctgcta ctggtggctg agcggatggg
    18661 cattgctcca caacagatgc tgtttgttgg cgactcacgc aatgatattc aggcagcaaa
    18721 agcggcaggt tgtccatcag ttggcttaac ctacggatat aactacggcg aggctatcga
    18781 tctcagccag cctgatgtaa tttatcagtc tataaatgac cttctgcccg cattagggct
    18841 tccgcatagc gaaaatcagg aatcgaaaaa tgactaagcc catcgttttt agtggcgcac
    18901 agccctcagg tgaattgacc attggtaact acatgggtgc gctgcgtcag tgggtaaaca
    18961 tgcaggatga ctaccattgc atttactgta tcgttgacca acacgcgatc accgtgcgcc
    19021 aggatgcaca gaagctgcgt aaagcgacgc tggatacgct ggcgttgtat ctggcttgtg
    19081 gtatcgatcc tgagaaaagc accatttttg ttcagtccca cgtgccggaa catgcgcagt
    19141 taggctgggc attgaactgc tatacctact tcggcgaact gagccgcatg acacagttta
    19201 aagataaatc tgcgcgttat gccgagaaca tcaacgctgg tctgtttgac tatccggtgc
    19261 tgatggcagc ggacatcctg ctgtatcaaa ctaatctggt gccggtgggt gaagaccaga
    19321 aacagcacct ggaactgagc cgcgatatcg cccagcgttt caacgcgctg tacggcgata
    19381 tctttaaggt gccagagccg tttattccga aatctggcgc gcgcgtaatg tcgctgctgg
    19441 agccgaccaa gaagatgtcc aagtctgacg ataaccgcaa taacgttatc ggcctgctgg
    19501 aagatccgaa atcggtagtg aagaaaatca aacgcgcggt cactgactcc gacgagccgc
    19561 cggtagttcg ctacgatgtg cagaacaaag cgggcgtttc caacctgctg gatatccttt
    19621 ctgcggtaac gggccagagc atcccggaac tggaaaaaca gttcgaaggc aagatgtatg
    19681 gtcatctgaa aggtgaagtg gctgatgccg tttccggtat gctgactgaa ttgcaggaac
    19741 gttaccaccg tttccgcaac gatgaagcct tcctgcaaca ggtgatgaaa gacggcgcag
    19801 aaaaagccag cgcacacgct tcccgtacgc taaaagcggt gtacgaagcg attggttttg
    19861 tggcgaagcc gtaatttaac attaatgcca aaaaccgggg aaacccggtt tttttatcct
    19921 catttgcaat aatccgaaaa aatgtgaagc gcctcgccgt ttccacatct cgctgttgcg
    19981 ccttatcttc aaattcctga agatataaac attcatttta ttgaaaatat attatgcgtc
    20041 gaacgtttat caaaaaagaa ggcgtcgtta tcacgacact ggcccgttat ttgttgggtg
    20101 aaaagtgcgg taatcgactg aaaaccatag atgagctggc aactgaatgc cgttcatcgg
    20161 ttggcctgac gcaggccgcg ttgaaaacgc tggaatcaag cggagcgata cggattgaac
    20221 gccgtgggcg caatggcagt tatctggtcg agatggataa caaagcattg ctgactcatg
    20281 tggatatcaa caacgtggta tgtgcgatgc ccttgcccta tacccgtttg tacgaaggcc
    20341 tggcgagcgg attgaaagcc cagtttgatg gcattccttt ttactatgcg catatgcgcg
    20401 gcgcggatat tcgcgtggag tgtttactta acggcgtgta tgacatggcg gtggtttcgc
    20461 gactggcggc ggaaagttat ctcacgcaaa aaggattatg cctcgcgctg gagttgggac
    20521 cgcataccta cgttggcgag caccagttga tttgccgtaa aggcgagtcc gcaaacgtga
    20581 agcgcgtggg gctggataac cgttcggcag atcagaaaat catgaccgat gtcttttttg
    20641 gcgatagtga tgtggaacga gtcgatctct cttatcacga gagtttacaa cgcattgtta
    20701 aaggcgatgt tgacgcggtt atctggaacg tagtggcgga aaacgaactg accatgctgg
    20761 gattagaagc gacgccgctc acagacgatc cgcgattttt acaggccacc gaagccgtgg
    20821 ttttgacgcg agtcgatgat tacccaatgc aacaattgct gcgtgcagtt gttgataaac
    20881 atgccctgct tgctcatcaa cagcgagtag tcagtgggga acaggaaccc agttattaaa
    20941 cgaaaggcat ctgatatgga aaccagactc aacctgcttt gcgatgcagg cgttattgat
    21001 aaggacatct gcaaaggcat gatgcaggtc gtcaatgtac tggaaacaga gtgccatcta
    21061 ccggtgcgca gtgagcaagg aacgatggcg atgacacata tggcgagtgc actgatgcgc
    21121 agtcgccgtg gtgaagaaat agagccgctg gatgacgaat tgctggcgga actggcgcaa
    21181 tccagccact ggcaagccgt tgtgcaattg catcaggtat tgttgaagga atttgcactg
    21241 gaagttaacc cgtgtgaaga aggctatttg ctggccaacc tttatggatt atggatggct
    21301 gctaacgaag aggtttgaag ctcccgataa atccacgtcg tacaacctaa atattcaaaa
    21361 aaatgaatat ttaattcaag gaacagaaac gaggcaacaa gatgtttgta gaagcattga
    21421 aacgccagaa tccggcgctg atttccgccg cactaagttt gtggcagcag ggcaagatcg
    21481 ccccggacag ttgggtgatc gacgtggatc aagttctgga aaacggcaag cggctgattg
    21541 agacggcgcg gctttacggc attgaactgt atctgatgac caaacaattc ggtcgtaatc
    21601 cgtggctggc agaaaaactg ctggctctgg gctacagcgg cattgtggcg gtggattaca
    21661 aagaggcgcg agtcatgcgc cgcgccggtt tgccggtagc gcatcagggg catctggtac
    21721 aaatcccttg tcatcaggtt tctgacgccg ttgaacaggg aaccgacgtc atcactgtgt
    21781 ttaccctcga caaagcgcgg gaaatttctg cggcagcggt gaagaccgga cgtgtgcagt
    21841 ctgtgctgct caaagtttat agcgacgatg attttcttta tccggggcag gagagcggtt
    21901 ttgtacagca ttcgctgcac gaggtggttg ctgaaatcca gaatctgccg gggctacatt
    21961 tggcgggact tacccatttc ccttgcctgc tttgggatga ggctgccggg aaagttttgc
    22021 cgacaccgaa tcttcacacg ctggtacaag cacgggatca actggcgaaa tctggtattg
    22081 cgattgagca actgaacgcg ccttcagcga ccagctgcac gtcgctgcca ttgctggcgg
    22141 aatacggtgt gactcatacc gaacccggtc atgcgctgac gggtactatt ccggcaaatc
    22201 aacagggcga tcagcctgaa cgtatcgcga tgctctggtt aagtgaaatc tcccatcatt
    22261 tccgtggcga cagctactgc tacggcggcg gttactaccg tcgtggtcac gcgcaacatg
    22321 cgctggtgtt tacgccagaa aatcaaagaa ttactgaaac atatctcaac gccgttgatg
    22381 acagcagtat cgactacacc ctgccgctgg caggcgagca tccggtaagc agtgcagtgg
    22441 tgctctgttt tcgcacgcag atttttatca cccgtagtga tgtggtgctg gtgtccggta
    22501 ttcaccgtgg cgaaccagaa atagttggtc gttatgacag cctcggcaac cctctggagg
    22561 cgtaaatggc gcgatttgtg gtgttagtga ttgatagctt tggcgtaggg gcaatgaaag
    22621 atgtcacgtt ggtgcgtccg caggatgccg gggcgaatac ctgtggtcac atcctgagcc
    22681 agttgccgca tttgcagcta ccaacgctgg agacgctggg gctaatcaac gcattgggtt
    22741 atgcgcctgg cgatatgcag ccgtcagact ccgcaacctg gggcgtggca gagctgcaac
    22801 atgaaggtgg cgataccttt atggggcatc aggaaatttt aggtacgcgc ccgttaccgc
    22861 cgctgcggat gccctttcgt gatgtgattg accgtgttga gcaggcatta gtttccgctg
    22921 gctggcaggt tgagcgtcgt ggcgatgatc tgcaatttct gtgggtcaat caggcggttg
    22981 cgattggcga taatctcgag gcggatttag gccaggtcta taacatcacc gccaatctct
    23041 ctgtgatctc ttttgacgac gcaatcaaaa tgggtcgtat cgtgcgtgag caggtacagg
    23101 tcggtcgggt cattacattt ggtggcctgt taaccgacag ccagcgcatc ctcgatgccg
    23161 cagaaagcaa agaagggcgc tttattggca tcaatgcgcc gcgttctggc gcctatgaca
    23221 acggtttcca ggtcgtacat atgggctacg gtgtcgatga aaaagtgcag gtaccgcaaa
    23281 aactgtatga agcgggcgtg ccaaccgtgc tggtgggtaa ggtggcagat atcgtcagca
    23341 atccttatgg cgtgagctgg caaaacctgg tggatagcca gcggattatg gatatcaccc
    23401 tcaacgaatt taacacctat ccgacggcgt ttatttgtac caatatccag gaaaccgacc
    23461 tcgctggtca tgcagaagac gtcgcacgtt atgccgaacg tttgcaggtc gttgaccgta
    23521 accttgccag gcttgttgag gcgatgcagc cagatgattg cctggtcgtg atggcggatc
    23581 acggtaacga tccgaccatt ggtcacagcc accatacccg cgaagtggtg ccagtgctgg
    23641 tttatcagca aggactggtc gctacgcagc tcggtgtgcg caccacgctt tctgatgtgg
    23701 gggctaccgt gtgtgaattt ttccgtgcgc caccgccaca aaatggtcgc tcttttcttt
    23761 cctccttccg gtttgcagga gacaccctat gagttttgat ccgacgggct acacgctcgc
    23821 ccatgagcat ctgcatattg atctttccgg ctttaaaaac aacgtggact gccgccttga
    23881 tcagtatgcg ttcatttgcc aggagatgaa cgacctgatg gcccggggcg tgcgtaatgt
    23941 gattgagatg accaaccgtt acatggggcg caatgcgcaa tttatgctcg atgtgatgca
    24001 cgaaactggc atcaacgtgg tggcctgtac cggttattac caggacgcgt ttttcccgga
    24061 acatgtggcg acccgcagcg tgcaggaact ggcgcaggag atggtcgatg aaattgagca
    24121 gggtatcgat ggcactgacc tgaaagccgg gctcatcgcg gagattggct ccagcgaagg
    24181 gaagattacg ccgctggaag agaaagtttt tattgccgcc gcgctggcac ataaccagac
    24241 cggacgcccg atctccacgc atacgtcgtt cagcacgatg gggctggagc aactggctct
    24301 gttgcaagcc cacggagttg atctttcgcg cgtcaccgtc ggccactgcg atctgaaaga
    24361 caatctcgac aacattttga agatgatcga tctcggcgcg tatgtgcagt tcgacaccat
    24421 cggcaagaac agttactacc cggacgaaaa acgcattgcg atgctccatg cgctacgtga
    24481 ccgtgggttg ctgaaccgcg tcatgctgtc gatggatatt acgcggcgct ctcatttaaa
    24541 agccaacggt ggttatggct atgacttttt attggccacc tttattccgc aattgcgcca
    24601 gtcaggattc agtcaggccg atgtggatgt gatgttacgt gaaaatccct ctcaattttt
    24661 ccaataagga cagactcatg aaaaagattg gcgttgcagg cttacagcgt gagcagatta
    24721 aaaaaaccat tgaagcgacg gctcctggct gttttgacgt tttcattcac aacgacatgg
    24781 aagcggcaat gaaagtgaaa tccgggcaac tggattacta catcggcgcg tgtaataccg
    24841 gtgcgggcgc ggcattgtcg attgccatcg cggtgattgg ctataacaaa agttgcacca
    24901 ttgccaagcc gggcattaaa gcgaaagacg aacatatcgc caaaatgatc gctgaaggaa
    24961 aagtggcgtt tggcctttcc gttgagcacg ttgaacacgc gattccgatg ctgattaacc
    25021 atctgaaata aaaggcacga ctatggatct gtatattcag attatcgtgg tggcgtgcct
    25081 gacgggtatg acatcgcttc tggcgcatcg ctcggcggcc gtttttcatg acggtatccg
    25141 cccgatcctg ccacaactga ttgaaggcta tatgaaccgt cgcgaggcgg ggagtatcgc
    25201 ttttggtctg agcattggtt ttgtggcctc ggtggggatc tcttttaccc tgaaaaccgg
    25261 gctgctcaac gcatggttac tctttcttcc taccgatatc ctcggcgtac tggcgataaa
    25321 cagcctgatg gcgtttggtc ttggtgctat ctggggaata ttgatcctca cctgtctgtt
    25381 gccagtaaac cagctgctga ccgcgctacc ggtggatgta ttaggtagcc tgggggaatt
    25441 aagctcgccg gtggtttcag cttttgcact gttcccgttg gtggcgattt tctaccagtt
    25501 tggctggaag caaagtctga tcgccgccgt ggtggtactg atgactcgtg tggtggtagt
    25561 gcgctatttc ccacatctta accctgaatc catcgaaatc tttattggca tggtgatgct
    25621 gctggggatc gcaataactc atgacctgcg ccatcgtgat gaaaatgata tcgatgccag
    25681 cggtatgtcg gtgtttgaag agcgtacatc acggattatc aaaaacttac cgtatatcgc
    25741 catcgtgggt gcattgattg ctgccgttgc cagcatgaag attttcgccg gtagtgaagt
    25801 gtcgatcttc acactggaga aagcgtactc cgcaggcgta acgccggaac aatcgcaaac
    25861 actaatcaac caggcggcac tggcagaatt tatgcgcggg ctgggttttt tgccgttgat
    25921 tgccaccacc gcgttagcca ccggtgtgta tgcagttgcg ggctttacct ttgtttatgc
    25981 ggtgggctat ctctcgccga atccgatggt tgcagcggta ttaggcgcag tggttatttc
    26041 ggcggaagtc ttgctgcttc gttcgatcgg caaatggttg gggcgctacc cgtcggtacg
    26101 taatgcgtcg gataacatcc gtaacgccat gaatatgctg atggaagtgg cgttgctggt
    26161 cggttcgatc tttgcggcaa tcaaaatggc gggctatacc ggattctcta ttgcggttgc
    26221 catttacttc ctcaacgaat ccctgggccg tccggtacag aaaatggcgg caccggtcgt
    26281 agcagtaatg atcaccggca tcctgctgaa tgttctttac tggcttggcc tgttcgttcc
    26341 ggcttaagga aggcacctat gaagacgttt cctctgcaaa gcctgacgct tattgaggcg
    26401 cagcaaaagc agtttgcgct ggtggacacg atatgccgtc attttcctgg cagcgagttt
    26461 cttacctgcg gtgatttggg cttaacgccg gggctaaatc agccccgcat tacccagcgt
    26521 gtggaacagg tgctggctga cgcgtttcat gcacccgcgg cggcgctggt acagggcgcg
    26581 ggtactggcg cgatccgtgc ggcactggcg gctttgctca aatcggggca acgtcttctg
    26641 gtgcatgacg cgccggttta tccaacgacg cgggttatta ttgagcagat ggggctaacg
    26701 ctcattacgg ctgatttcaa tgatctgtcg gcgcttaaac aaatcattga agcgcaacaa
    26761 cctgatgcag cgctggtgca gcatacgcgc cagcagccgc aggaccgcta tgtgctggca
    26821 gacgtgctgg caacgttgcg cgcggcaggt gttccagcgt taaccgatga caactatgcg
    26881 gtgatgaagg tagcgcgaat cggctgcgaa tgcggggcga atgtgtcgac attttcctgc
    26941 ttcaagctgt ttgggccaga gggcgttggt gcggtggtag gcgatgccga tgttattagc
    27001 cggattcgcg ccacgctcta ttccggcggc agtcagattc agggggcgca ggcattagag
    27061 gtgttgcgtg gtctggtgct cgcgccagtg atgcacgcgg tgcaggcggg ggtatctgaa
    27121 cggttgctgg ctttgcttaa cggcggcgcg gtggcggaag tgaaaagcgc cgtcattgcc
    27181 aatgcacagt cgaaggtatt gattgtggag tttcatcagc cgattgccgc cagagtgctg
    27241 gaagaggcgc agaaactcgg tgccttgcct tacccggttg gtgcagagtc gaaatatgaa
    27301 atcccgccgc tcttttatcg actttccgga acgtttcgcc aggcgaatcc gcaactcgaa
    27361 cattgcgcga ttcgcattaa cccaaatcgc agcggtgaag agacggtgct gcggattttg
    27421 cgtgagagta ttgccagtat ttaatcttgt gcatgatgtt ttcgaaaaga atgcagaaat
    27481 aaatacgccc tcgggtaacc ccgagggcgt caggcaatta aaccggtgtt agccgatttc
    27541 tgtcagagac ttactgtgca gtaggaccgc aaccgccaat gattttagaa atagaaattg
    27601 ccgggtgcag caggtagtcg taagagcagt tcttatcacg gttttcaata tgaccagtac
    27661 atgcagttaa cgtagcgagt actgcgccta ccagtgcaac tttaataaat ttgttc
//
LOCUS       DAZFJL010000059        27445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFJL010000059 DAZFJL010000000
VERSION     DAZFJL010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.059"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            228..341
                     /gene="yhiY"
                     /locus_tag="ACHJ8C_002382"
     CDS             228..341
                     /gene="yhiY"
                     /locus_tag="ACHJ8C_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO5552227.1"
                     /translation="MMTTLLPVFTKPSPLALNALCAGRICHFLLIPDGRIR"
     gene            404..1471
                     /gene="yhiI"
                     /locus_tag="ACHJ8C_002383"
     CDS             404..1471
                     /gene="yhiI"
                     /locus_tag="ACHJ8C_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO5552228.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1468..4203
                     /gene="rbbA"
                     /locus_tag="ACHJ8C_002384"
     CDS             1468..4203
                     /gene="rbbA"
                     /locus_tag="ACHJ8C_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO5552229.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTAALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGGVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQAENAETA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPPELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4203..5327
                     /gene="yhhJ"
                     /locus_tag="ACHJ8C_002385"
     CDS             4203..5327
                     /gene="yhhJ"
                     /locus_tag="ACHJ8C_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5552230.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQADIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            5400..5675
                     /locus_tag="ACHJ8C_002386"
     CDS             5400..5675
                     /locus_tag="ACHJ8C_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO5552231.1"
                     /translation="MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            5672..6031
                     /locus_tag="ACHJ8C_002387"
     CDS             5672..6031
                     /locus_tag="ACHJ8C_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO5552232.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6151..6552)
                     /gene="nikR"
                     /locus_tag="ACHJ8C_002388"
     CDS             complement(6151..6552)
                     /gene="nikR"
                     /locus_tag="ACHJ8C_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO5552233.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(6558..7364)
                     /gene="nikE"
                     /locus_tag="ACHJ8C_002389"
     CDS             complement(6558..7364)
                     /gene="nikE"
                     /locus_tag="ACHJ8C_002389"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO5552234.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLALKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(7361..8125)
                     /gene="nikD"
                     /locus_tag="ACHJ8C_002390"
     CDS             complement(7361..8125)
                     /gene="nikD"
                     /locus_tag="ACHJ8C_002390"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO5552235.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(8125..8958)
                     /gene="nikC"
                     /locus_tag="ACHJ8C_002391"
     CDS             complement(8125..8958)
                     /gene="nikC"
                     /locus_tag="ACHJ8C_002391"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO5552236.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLIIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGAGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(8955..9899)
                     /gene="nikB"
                     /locus_tag="ACHJ8C_002392"
     CDS             complement(8955..9899)
                     /gene="nikB"
                     /locus_tag="ACHJ8C_002392"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO5552237.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(9899..11473)
                     /gene="nikA"
                     /locus_tag="ACHJ8C_002393"
     CDS             complement(9899..11473)
                     /gene="nikA"
                     /locus_tag="ACHJ8C_002393"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5552238.1"
                     /translation="MFSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(11584..12171)
                     /gene="acpT"
                     /locus_tag="ACHJ8C_002394"
     CDS             complement(11584..12171)
                     /gene="acpT"
                     /locus_tag="ACHJ8C_002394"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO5552239.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYNSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(12173..13402)
                     /locus_tag="ACHJ8C_002395"
     CDS             complement(12173..13402)
                     /locus_tag="ACHJ8C_002395"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFO5552240.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(13399..14130)
                     /locus_tag="ACHJ8C_002396"
     CDS             complement(13399..14130)
                     /locus_tag="ACHJ8C_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFO5552241.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGTRQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            complement(14130..14594)
                     /locus_tag="ACHJ8C_002397"
     CDS             complement(14130..14594)
                     /locus_tag="ACHJ8C_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFO5552242.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFSLELMAQTVGVWSGWHRRQQGKNSIQLGMVLGARELLCAAGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTEEELTTLFQQGAS"
     gene            complement(14591..15760)
                     /locus_tag="ACHJ8C_002398"
     CDS             complement(14591..15760)
                     /locus_tag="ACHJ8C_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFO5552243.1"
                     /translation="MIYISAVGMINALGNNLDDIAANLTRGVAPGMRPRAGWLQGHQQ
                     AVLAGVDGELPVIPEKFAAHRSRNNQILLAALAQIQPQVDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTNGQPSTTWHYAQQELGDPSRFLSHWLVLDGPAYTLSTSCSSSA
                     RAMISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGAAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            complement(15762..16346)
                     /locus_tag="ACHJ8C_002399"
     CDS             complement(15762..16346)
                     /locus_tag="ACHJ8C_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFO5552244.1"
                     /translation="MIKHTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAKG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPTSWTLRDNGNKRELRNASGKL
                     VTEIIYMNRQGKRVPISIEQHVFKYHITIQYLGD"
     gene            complement(16343..18661)
                     /locus_tag="ACHJ8C_002400"
     CDS             complement(16343..18661)
                     /locus_tag="ACHJ8C_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFO5552245.1"
                     /translation="MTNANALPPSKRPALLWGLVCLVMAAALLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPRVAQEWLTLLQKSAALGDIKG
                     PMDAASQQAWGAFFWQHRNGLIDPDTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTVATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSRGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKFTTSLEKA
                     KKDGLISKYRTIPLNSLARQEEDLHLLTTAAPTVTKALQNAGLTTVNPDLNAMPVKVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALLQEITSYYPCGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVITCGAIARLGWRKGLISLVPSVLSLGCGLAVLAISGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAITLAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            complement(18630..19235)
                     /locus_tag="ACHJ8C_002401"
     CDS             complement(18630..19235)
                     /locus_tag="ACHJ8C_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFO5552246.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWNQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFHVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGQ
                     TYLESIQLNDKQGDRTDIALIQHQLTPAQLTDDERQRFATQ"
     gene            complement(19232..19654)
                     /locus_tag="ACHJ8C_002402"
     CDS             complement(19232..19654)
                     /locus_tag="ACHJ8C_002402"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFO5552247.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKP"
     gene            complement(19658..21334)
                     /locus_tag="ACHJ8C_002403"
     CDS             complement(19658..21334)
                     /locus_tag="ACHJ8C_002403"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO5552248.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAT
                     TQQQLDSLLAEQPGVTLIRLAENAGKGAAVMRGLQAAAEAGFSHAVQVDADGQHAIED
                     IPKLLALAEQHPAVLISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKRATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPQDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTAARARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQLGRDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVN
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCETFADP
                     LLLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            complement(21325..21678)
                     /locus_tag="ACHJ8C_002404"
     CDS             complement(21325..21678)
                     /locus_tag="ACHJ8C_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFO5552249.1"
                     /translation="MKPHEIERHQAQANHLEIVLHLRADLFWFRGHFAVQPLLPGVAQ
                     IDWAMSYALTLLAPGWRFHSIQNIKFQSPLLPENRVTLTLNWQEERQILSFSYQRHDG
                     DARHTASSGKIRLCR"
     gene            complement(21665..23026)
                     /locus_tag="ACHJ8C_002405"
     CDS             complement(21665..23026)
                     /locus_tag="ACHJ8C_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFO5552250.1"
                     /translation="MKQTLPLARWLTAPRPDDTPIAWLNESTWTLGDLRHDVAQLICR
                     LQQQPGERWALCFENSYLFIVALLATLHAGKTPVLPGHNRVIQLNEQRELFDGVLSDS
                     ELNWQGSLLLVASSPQIATQSFTFAAIAPEAFIELFTSGSTGQPKRVIKPVHLLDREA
                     ELLAERLGARLAGCRVVGSVLPQHLYGLTFRVFLPMVLGLPLHAAMLWYVEQFAALSH
                     QHRYIFISSPAFLKRLDTQLSPPPVQVLLSAGGELPWQDVQHTASWLRVWPDEIYGST
                     ETGVIAWRYREQEQRRWLPFPGMQFQAEGDSFRLFSPLMAEDNGLLLDDLLQFSQDGQ
                     FHLMGRRGRIVKIEEKRISLQEVEQRLLALDGIREAAAVPVTRGGRQSIGVLLVLDDE
                     AHLQWQNGSGHSQEMTWRRLLRPTLEPVAIPRYWRVIDEMPVNSMNKRVYAQLQELFH
                     EAP"
     gene            complement(23023..23604)
                     /locus_tag="ACHJ8C_002406"
     CDS             complement(23023..23604)
                     /locus_tag="ACHJ8C_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5552251.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFVIWFGLAHNGLHWLLPLMALL
                     LLLRLRQTRRQAGPLQAVTQLVAVVGIALCVASFLLKTHQLLLFYPVVVNAVMLAVFG
                     GSLWSAMPIVERLARLQEPDLPEKGVRYTRHVTQIWCGFFIINGGIALFTALYADMSL
                     WTAWNGMIAYLLMGTLMAGEWLVRRQVMKRDRA"
     gene            complement(23609..23860)
                     /locus_tag="ACHJ8C_002407"
     CDS             complement(23609..23860)
                     /locus_tag="ACHJ8C_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO5552252.1"
                     /translation="MTEQQTVYQEVSALLVKLFEIDPQDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEA"
     gene            complement(23872..24129)
                     /locus_tag="ACHJ8C_002408"
     CDS             complement(23872..24129)
                     /locus_tag="ACHJ8C_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFO5552253.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIETDAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            complement(24104..24925)
                     /locus_tag="ACHJ8C_002409"
     CDS             complement(24104..24925)
                     /locus_tag="ACHJ8C_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFO5552254.1"
                     /translation="MSKLMSRLEWAWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILHQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPDRPFF
                     TVAVHGRVEIHKFYDATSQEPALAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            complement(24922..25644)
                     /locus_tag="ACHJ8C_002410"
     CDS             complement(24922..25644)
                     /locus_tag="ACHJ8C_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFO5552255.1"
                     /translation="MKFTLNITDWQALAPGLSEAQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPIVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPAKMPTWPYAVALVIEAGDDWQCETQPAIAGNETTLPQSMLFLQH
                     YLQNADTFSLSGERVQWRWSRR"
     gene            complement(25685..26743)
                     /locus_tag="ACHJ8C_002411"
     CDS             complement(25685..26743)
                     /locus_tag="ACHJ8C_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO5552256.1"
                     /translation="MYEQDSLSALDAITEAQRIAFAPMLFQTALCLRNAGVLSYLDRQ
                     GKHGATLADITEHSHINEYAASVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWSTIYPALSQLPDAARESWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            complement(26812..27189)
                     /locus_tag="ACHJ8C_002412"
     CDS             complement(26812..27189)
                     /locus_tag="ACHJ8C_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5552257.1"
                     /translation="MKWFAAIAVVGALAGCARTAPIDQVHSTVSAGHTQEQVKKAILK
                     AGVERKWIMSEAGQGIIKARQQSRDHSAEIRINYTASSYDINYENSQNLQASGGQIHK
                     NYNRWVRNLDKDIQLNLSAGADL"
BASE COUNT         6271 a         7417 c         7683 g         6074 t
ORIGIN      
        1 gataataacg ctatgtgata gtcatcaggt ttccctgtta cggaaacctg tgcaacagaa
       61 tctgtgaacc tgtacagaat atttttggaa aaaacaccaa cgaacggaag aacatcgcga
      121 tgtatcacaa gttgatggca atcaaataga caacatgaat ataactgtta gtatttaatt
      181 gatttcactt atgagtatgc gtgtttcatt tacggccagg aatgctgatg atgacaacat
      241 tactacccgt tttcactaag ccttccccat tggcgctcaa tgctctgtgc gctggtcgta
      301 tttgccattt ccttcttatc ccggatggaa gaatccgctg aacgtcgttc ggtggcagtt
      361 gtaacacgcg tcgattgctt acagggatta tggcgggtcg attatggata agagtaagcg
      421 ccatctggcg tggtgggttg tcggggcact ggcggtggct gccgtcgtgg cgtggtggct
      481 gttgcgcccg gcgggtgtgc cggaaggctt tgctgtcagc aatgggcgca ttgaagcgac
      541 ggaagtggat atcgccagca aaattgccgg gcgtatcgac accattctgg tgaaagaagg
      601 ccagtttgtt cgagaaggtg aagtgctggc gaagatggat actcgcgtgt tgcaggaaca
      661 gcgactggaa gccatcgcgc aaatcaaaga agcacaaagt gccgttgccg ccgcgcaggc
      721 tttgctggag caacgccaaa gcgaaactcg tgccgcacag tcgctggtta atcaacgcca
      781 ggcagaactg gactccgtag caaaacgtca tacgcgttcc cgctcactgg cacaacgggg
      841 ggcaatttct gcgcaacagc tggatgacga ccgcgccgcc gctgagagcg cccgagctgc
      901 gctggaatcg gcaaaagctc aggtttctgc ttctaaagcc gctatagagg cggcacgcac
      961 caatatcatt caggcgcaaa ctcgcgtcga agcggcacaa gccactgaac ggcgcattgc
     1021 cgcagatatc gatgacagcg aactgaaagc cccgcgtgac ggacgcgtgc agtatcgggt
     1081 tgccgagcca ggcgaagtgc tggcagcagg cggtcgggtg ctgaatatgg tcgatctcag
     1141 cgacgtctat atgaccttct ttctgccaac cgaacaggcg ggcacgctga aactgggcgg
     1201 tgaagcacgg ctgatcctcg atgccgcgcc agatctgcgt attcccgcga ccatcagttt
     1261 tgtcgccagc gtcgcccagt tcacgccaaa aaccgtcgaa accagcgatg aacggctgaa
     1321 attgatgttc cgtgtcaaag cgcgtatccc accggaatta ctccagcagc atctggaata
     1381 tgtcaaaacc ggattgccgg gcgtagcgtg ggtgcgggtg aatgaagaac ttccgtggcc
     1441 tgacgacctg gtggtgaggt tgccgcaatg acgcatctgg aactggttcc cgtcccgcct
     1501 gtcgcgcaac tggcgggcgt gagccagcat tatggaaaaa ccgctgcgct gaacaatatc
     1561 acactcgata ttccggcccg ttgcatggtc gggctgattg gaccggatgg tgtcggtaaa
     1621 tcgagcttgt tgtcgttgat ttccggtgcc cgcgtcattg agcagggcgg cgtgatggtg
     1681 ctgggcggcg atatgcgcga cccgaagcat cgccgcgacg tctgcccgcg catcgcctgg
     1741 atgccgcagg ggctgggcaa aaatctctac cacaccttgt cggtgtatga aaacgtcgat
     1801 tttttcgccc gcctgttcgg tcacgacaaa gcggagcggg aagtgcgaat taatgagctg
     1861 ctgaccagca ccggattagc accgtttcgc gatcgtccgg cagggaaact ctccggtggg
     1921 atgaagcaaa aacttgggct gtgctgtgcg ttaatccacg acccggaatt gttgattctt
     1981 gatgagccaa caacgggggt tgacccgctc tcccgctccc agttttggga tctgatcgac
     2041 agcattcgcc agcggcagag caatatgagc gtgctggttg ccaccgccta tatggaagag
     2101 gcggaacgct tcgactggct ggtagcgatg aatgccggag aagtgctggc aaccggtagc
     2161 gccgaagaac tgcggcagca aacgcaaagc gccacgctgg aagaagcatt tataaatctg
     2221 ttaccgcaag cacaacggca ggcgcatcag gcggtagtga tcccaccgta tcaagctgaa
     2281 aacgcagaga ctgccatcga agcgcgcgat ctgaccatgc gttttggttc cttcgttgcc
     2341 gttgatcacg tcaatttccg cattccacgc ggggagattt ttggttttct tggttcgaac
     2401 ggctgtggta aatccaccac catgaaaatg ctcaccggac tgctgcccgc cagcgaaggt
     2461 gaggcgtggc tgttcgggca accggttgat ccaaaagata tcgatactcg tcgccgggtg
     2521 ggctatatgt cgcaggcgtt ttcgctctat aacgaactca ccgtgcggca aaacctggag
     2581 ttacatgctc gtctgtttca tatcccggaa gcggaaattc ccgcgcgagt ggctgaaatg
     2641 agcgagcgtt ttaagctcaa cgacgtcgaa gatgtattgc cggagtcatt gccgctcggc
     2701 attcgccagc ggctatcgct ggcggtggcg gtgattcatc gcccggagat gctaatcctc
     2761 gatgagccga cttctggcgt cgatccggtg gcgagggata tgttctggca gttgatggtg
     2821 gatctctcgc gtcaggacaa agtgactatc ttcatctcca cccactttat gaacgaagcg
     2881 gaacgttgtg accgtatctc actgatgcac gctggaaaag tgctcgccag cggtacaccg
     2941 ccggaactgg ttgagaaacg cggagccgcc agtctggaag aggcatttat cgcttatttg
     3001 caggaagcgg cagggcagag caacgaagcc gaagcgccgc ctgtggtaca tgacactacc
     3061 cacgcgccac gtcagggatt cagcctgcgc cgtctgttta gctacagccg ccgcgaagcg
     3121 ctggaactgc gacgcgatcc ggtacgttcg acgctggcgc tgatgggaac ggtgatcctg
     3181 atgctgataa tgggttatgg catcagtatg gatgtggaaa acctgcgctt tgcggtgctt
     3241 gaccgcgatc aaaccgtcag tagccaggcg tggacgctca atctctccgg ttcccgttac
     3301 tttatcgaac agccgccgct caccagttat gacgagcttg atcgtcggat gcgtgcgggc
     3361 gatatcacgg tggcaattga gatccctcct aatttcgggc gcgatatcgc gcgtggtacg
     3421 cccgtggaac tcggtgtctg gatcgacggt gcgatgccga gccgcgccga aacggtaaaa
     3481 ggctacgtgc aggccatgca ccagagctgg ctacaggatg tggcgagccg acagtccaca
     3541 cccgccagcc aaagcgggct gatgaatatt gagacgcgct atcgctataa cccggacgta
     3601 aaaagcctgc cggcgattgt tccggcggtg atcccgcttc tgctgatgat gatcccgtca
     3661 atgctaagcg cccttagcgt ggtgcgggaa aaagagctag ggtcgattat caacctttac
     3721 gtgaccccca ccacccgtag cgaattttta cttggcaaac agctgccata catcgcgctg
     3781 gggatgctga actttttcct gctctgcgcc ctgtcggtgt ttgtgtttgg cgtaccgcat
     3841 aaaggcagtt tcctgactct caccctggcg gcgctgctgt atatcatcat cgccaccgga
     3901 atggggctgc tgatctccac ctttatgaaa agccagattg ccgccatttt cggtacggca
     3961 attatcacgt tgatcccggc gacacagttt tccgggatga tcgatccggt agcttcgctg
     4021 gaaggtccag gacgttggat cggcgaggtt tacccgacca gtcattttct gactatcgcc
     4081 cgcggaacgt tctcgaaagc gctggatctg actgatttgt ggcaactttt tatcccgttg
     4141 ctgatagcca taccgctggt gatgggccta agcatcctgc tgctgaaaaa acaggaggga
     4201 tgatgcgcca tttacgtaat atttttaatc tgggtatcaa agagttgcgc agtctgctcg
     4261 gtgataaagc gatgctgacg ctgattgtct tctcgtttac ggtatcggtg tattcgtcag
     4321 cgaccgttac gccaggatcg ttgaacctcg cgccaatcgc cattgccgat atggatcaat
     4381 cgcagttatc gaaccggatc gttaacagct tctatcgccc gtggtttttg ccaccggaga
     4441 tgatcaccgc cgatgagatg gatgccgggc tggacgccgg acgttatacc ttcgcgataa
     4501 atattccgcc taattttcag cgtgatgtcc tcgccggacg ccaggcggat attcaggtga
     4561 acgttgatgc cacgcgcatg agccaggcat ttaccggcaa tgggtatatc cagaatatta
     4621 tcaacggtga agtgaacagc tttgtcgcgc gctaccgtga taacagcgaa ccgttggtat
     4681 cgctggaaac acggatgcgc tttaacccga accttgatcc cgcgtggttc ggtggggtga
     4741 tggcgatcat caacaacatt accatgctgg cgattgtatt gaccggatcg gcactgatcc
     4801 gcgagcgtga acacggcacg gtggaacact tactggtgat gccgataacg ccgtttgaga
     4861 tcatgatggc gaaggtctgg tcgatggggc tggtggtgct ggtggtgtcg ggattatcgc
     4921 tggtactgat ggtgaaaggc gtgctgggcg taccgattga aggctcgatc ccgctgttta
     4981 tgctgggcgt ggcgctcagt ctgtttgcca ccacgtcaat cggcattttt atggggacga
     5041 tagcgcgttc aatgccgcaa ctggggctgc tggtgattct ggtgctgctg ccgctgcaaa
     5101 tgctttccgg tggttccacg ccgcgcgaaa gtatgccgca gatggtgcag gacattatgc
     5161 tgaccatgcc gacgacacac tttgttagcc tcgcgcaggc cattctctac cgtggtgccg
     5221 gattcgaaat cgtctggccg cagttcctga cgctgatggc aattggcggc gcgtttttca
     5281 ccatcgcact gctgcgattc aggaagacga ttgggacaat ggcgtaagga agggaaactt
     5341 gacttcatat tgtgatgtca tactattgat gtcataaagt gaagtcataa ggacatgcca
     5401 tgagaaccca ggtacaggct ttacggaaga aacaaaaaaa tacattggat caaatattta
     5461 aatctcctgt tcctcaaggg attaagtggt ctgatataga gtcactggtt aaagcattag
     5521 gcggagaaat taaggaagga agaggttcgc gttgtaagtt catactaaat atgagcgttg
     5581 cgtgtttcca tcggcctcat ccgtcgccag ataccgataa aggcgctgta gaaagcgtgc
     5641 gtgactggtt actaagtata ggggtaaaac catgatcaaa ctgaagacgc caaactctat
     5701 ggagattgcc gggcaacctg ctgttatcac ttacgtgcca gaactgaatg catttcgtgg
     5761 caaattcctg ggcttgtcag gttattgtga ttttgtctct gacagcattc aggggctgca
     5821 aaaagaaggg gagctttccc tgcgagagta tcttgaggat tgtaaagctg cgggtattga
     5881 gccgtatgcg cgtacagaaa agattaagac ctttacatta cgctatccag aatcgctgag
     5941 tgaacgcctt aataatgcgg ctgcgcagca acaagtctct gtaaatactt atattattga
     6001 aacgctaaat gagcgtttga atcatttata aattttagcg tcgtacatta cgcagaatat
     6061 ctgcctttac aggatctatc tatgcaactg aacttaattt tataaattaa gttcagttaa
     6121 tatcttttct ttcctcgttt tagaaataac tcaatcttcc ttcggcaggc actgcaaatg
     6181 cccgtgccgc acgccgcgct gggcgatgac gtcatcggca aaatgctgca cgtcgcccat
     6241 gtcacctttc aacacggcga tttccagaca gtcgtcgtga ttaatgtgca catgcagagt
     6301 agcaactgag agatcgtggt gatgatgctg ggtggagaca atacggctgg ctaagtcgcg
     6361 tttttcgtgt tcatacacat acgacagcac cgcgaaacct tgtgtgccgt gttgttgagt
     6421 ggcttcctgc gccagagcgc tacgcagaat atcgcggatc gcctcggaac ggttgttata
     6481 accacgacgc tggctcagac tgtccagcgt ctccagtaaa tcgtcatcaa gcgtgatggt
     6541 gactcgttgc atttgcgtta aaccttttct gtggtgcgac ggcgcacggg gaatgcgggt
     6601 aataccgcgt tttgtagcac acgtccggcg tcagaggaaa aggttaattt atctcccacc
     6661 acctgggttt cgacgatttg tccgttgtcc atcaccatca cccgctgaca gaagcgttcc
     6721 acaaggcgta agtcgtgggt gatgaacaag caggcggtgc caaactgttg ttgcagcttt
     6781 ttcagcaggc gaatgacgcc cgcctgtaac acgagatcaa ggttcgaaac cgcttcatcc
     6841 agaatcagca gtttcggttc gaccgccagc gcgcgtgcca ggcagacacg ctggagctgg
     6901 ccgccgctta actgcggtgg gcgtttgtcg agcacgctgt cgtcgagatc gaccgccttc
     6961 agcatttcac tggcgcgcgc cagttgttcg gcttttttca gcgatagcag gtggcgcatc
     7021 ggttcacgca ggatctcgcg cacggttttg cgcggattca cggcgctgat ggagtcctga
     7081 aacaccattt gaatatcacg gcggaacgct ttacgttgcg cgcgattgag cttcgccaac
     7141 ggttcgccgc gccagctaat atttccctgt gagggcgact ctaaccctac cagcagccgc
     7201 gcgagggtac tttttccgca gccgctgcgc cccagcaggg cgacggtttc accgcttttc
     7261 agggccaggg aaacgttatt cagcaccgcc tggtgttgat gtttgccgct aaatccaccg
     7321 tgcgcgtagt gatgggaaag gtcggagacg ttaagtaaag tcatgatgcc agctccatac
     7381 cgtagagggc gagatgcgcg gaaaccaggc tgcgcgttac cgcatgtttg ggggcgttaa
     7441 acagcgtttc tacatcgccc tgttcaacaa tcttaccgtg agacattacc gccacatcgt
     7501 ctgccagacg cgctaccacg cccatatcat gggtaaccag cagcattccc ggtgcttgtt
     7561 tttgcataat gctttccagc agatcgagga tgcgcgcctg tgctaccacg tcgagatcgg
     7621 tggtcggttc atcggcgatg ataaacggtg attcgcacag caccgccatc gcaatcatca
     7681 tgcgctgcaa catgccgccg ctcatctcga acgggtagag cttcagcacg cgcgcggcgt
     7741 tttccagccc caccgcttct atggcagcgg taagcgtagc gtcatcggcg ggtttcccca
     7801 gcgccaggca ggtttcacgc gcgtgggtgt gcatggtgtg cagcggatta aaggcactgc
     7861 gcgggttctg catgatggtg gcaattttga ttccgcgcag ggcgcaggga gaaaccggtt
     7921 ttccatcggc caaaatttcc cccgccgtct ggcgaacgcc agcaggcaga atgcccagcg
     7981 tcgcggcgca ggtcagcgac ttcccgctgc cactaccgcc gactaacgcc agcacacgcc
     8041 cacgttgcag ggttaacgat acgccgtgca ccagcggctg cgcggcctgt agcgcgatat
     8101 tacggagttc aatctgttgt ggcattagtg tgcgtgctcc gtcaccagat gagggtccag
     8161 atgatcgcgc agtgcgtcac ccaccaggtt aaaggccatc acgctgataa acagcgccag
     8221 ccccggccag aacatttgca gcggctgggt ccagatatac tgacgcgcgt cgttaatcat
     8281 cacgccccat tcggcggtcg gcgcggtcac gccgagaccg aggaaggaca tccccgcgac
     8341 gtgtagcatc atatgaccga tatccagcgt cgccagcacc agcagcgagg ggatcaccgc
     8401 acctgccaga tgatcaacaa acacccgcac atggcccgcg ccagaaagcc gtgatgccag
     8461 cacaaactcg cgctggcgca gtgaaatcac caggctacgc accatgcgtg cataccacgc
     8521 ccagtgcgac agggcgatgg ctataatcac attggtcaac ccggcgccga gcacgccgac
     8581 cataaagaac gacagaatcg aggttgggaa ggtcataaac atatcagcga cgcgcatggt
     8641 ggcctgatca acgcgcccgc caatcagccc ggcgctgccg ccaataatca gccctaatgt
     8701 cagcaccaac agcaggcagg ccattaccga gccgagcgac acgcgggtcg ctgccatcag
     8761 ccgcgagaaa atatcgcgcc ctaaatgatc ggtgcccagc cagtgctgcg catccggcga
     8821 gagcaggcgc gacggcaaat caatagcctg agggtcatac ggcagccacc actggctggt
     8881 gagcgcaatc agcgccagca gggcgataat gatcagcgcc aggcgaaccg accagcggga
     8941 ggagaggaaa aagttcacgc gtgcgctcct tcatgacgac gaatacgcgg gtccagcgcg
     9001 gcgttgagca aatcgacaat caaattacag accacaaaaa ccaccaccat catcagcgta
     9061 aagcattgga tcaccggata gtcacggtta aaaatcgccg acaccgcata gcgcccgacg
     9121 cccggccagg caaagatgtt ttcgataatc atcgtcccgc caatcagttc gccaatgtgc
     9181 atccccacgg cagtgatcat cggcagcgag gcattgcgca gaatatggcg gcgttcggtc
     9241 tgtttgtcat tcaggccgcg cagccgcgcc caggtcacat gacgctgacc ggcgacgtcc
     9301 agcatactgg cgcgcagcaa acgcgcgtta atcgccagcg acataaaggc gatggatacc
     9361 gcaggcaaaa tgatgtgctg ccagccgccg taacccatcg cgggtagcca ttgcagatac
     9421 accgaaaacg ccatcaccag taaaaacgcc agccagaagt taggcatcga cacgccgaga
     9481 aacgcgatga aacggacggc gaaatccggc agacgatcgc gatggcgcgc cgcccagata
     9541 ccgagcggca cggaggtgag cagaattaat accagcgccg cacctgcaag ttccagcgtg
     9601 gcgggcagga agttcagcat atcgtccagc accgggcgtt ggctggcgaa tgagatacca
     9661 aagtcaagat gcagcgcctt ccacaaccag gtgccgtact ggacatacag cggctgatcc
     9721 agccccagca tagtgcgggt agaggccagc atctccggcg tcggcggcag gttagacaga
     9781 cggagataat cgagcgccgg gtcaccggtg ccgaggcgca gcatcagaaa aatgatcacc
     9841 gaggcggcga gcaccatcgg gatcagcagc agaaagcggc gtaatacata acgcaacatt
     9901 aaggtttcac cggtttaatc tgttcgaacg ggatttcggt ggcgatcggc gcgtagggga
     9961 tgttacccag ttccggtttt gaaaccacca tcattgagat gtaactgata ggcagataaa
    10021 ccgcctcgtc atgcagacgg gtcagaatgt cgcgatacag cgcctgacgt tgcgtttcgt
    10081 catgggtcgc cagcacttcg ccgatctctt tatcaatcag cggcttgtcg gctaaacctt
    10141 gctgtgcctg gaagtcagcg tgcgacggta cgcgcattga actcaggaag gcgtgtggat
    10201 cgtatggcgc gccccaggtg cggtggaaaa tcatgccaaa acgaccgtcg cgctgacgag
    10261 catagatact gctctcttct tcgccaatca gcgagacatc tgcgccaatc tggcgcatat
    10321 cagcctgaat gatttccgcc atcgatttgc ttaacgcatc ggtgccgatg aacgacagtt
    10381 caatgcgcag cggctgacca tttttctcgc ggatgtcttt gcctgcaggc agcgtccaac
    10441 cggctttttc cagcaacgct ttcgctttct gcggatcgta ctggcgcggt ttcaggccga
    10501 ggttggcgta gggcacagaa ggggcaaaca gggtgtcggc gacctgctgg gtaccataca
    10561 acgcgttatc aatcagcgat tttttgttca ccgcgtaatt aagagcttca cgtactgcca
    10621 gctcgttggt gggggctttg gcggtattga gcgccagcat cacggtttcg atcggctgtg
    10681 acagttgagt gtggtaagcc gggttctggc taaagcgggc gaaggtatcg agcggtaata
    10741 acccttcgtt tccgtacagc aggtcgatat cgccagtttc aaacgccacc gcgcgggtag
    10801 tcgggtccgg gatcacgtta aaggtgatct ttttaatcgc tggcttttcg ccccagtagt
    10861 tttcgttacg gacgaagaca tcgtactgat tcagtttcga ttcctgtaaa acccacggcc
    10921 cggtgccaat cggcgcttta attccgttca tggtttcgtg gtttttaaac tgcgagggtg
    10981 cgataaaacg gaaaggacgg ggcagggcca gttcttgcag gaaaggatag taggcgcttt
    11041 tcagggtgat ttgcagctcg tttttgctga gtgctttgac atcaacaatc tggtttgcca
    11101 gctccagcca ggcgtgacgt tgacggttat cgagcactgc acggaagttt tctgccgccg
    11161 cctctgcatc aaacggttcg ccgttggaga atttcacgtc atcacgcagg gtgaaggtcc
    11221 aggttttacc atcttctgaa tgagtccagc tttttgccag ccacgggatc accgaaccgt
    11281 ctgcctgata tttcaccagc ggttcataaa ccatgctctg ggcgaacatt tggttaggcg
    11341 tgtaaaggtg cgggtttagt ggcccgacat tcaccggcca ggcggtggtg atttcatctg
    11401 gtgcagcggc atggacgata aaagacgcac aagccagcag cgcaaataga gtgcggcgga
    11461 gtgtagagaa catggtaacc ccaatggatt aaaatagatg gcggaaataa gtatgacgat
    11521 tttaagtatt cgtcatactg attacctgtt ctggatcaat agatgggcag tcgggtgggc
    11581 ggatcagtta actgaatcga tccattgcac actgtcggcg gtgagcgtaa agggggtagg
    11641 ggtgcagatc gccaggctta aattttctag ctgacaatgg ctgactgaca gcgaggagtt
    11701 ataggtgctg tctacgctga cgatttgcca ggcgctgccg ccacgctgtt taacgatggc
    11761 ttctttgcgc gtccagatgc gccagaacat ttccagttgc tgatcaggat gcacggcgtc
    11821 catctcggca tgttccccaa ggctgaatac ggcgttcgcc agccagcgcc agttggcgcg
    11881 cgggcgaatc acttcgatat cgcagccgac ttcgccttca tcgctcaaca gtagggcgat
    11941 atcgtcaccg ctatggctta agttgaacca tagcggcgtt tccggcgcga atgcaggttt
    12001 gccttgttcg ccatagatga tctccggtag cggggaaagc gtgtgcgaaa gcaatgcacg
    12061 ccccgccagc cagcgttcgc gtcgtggacc ttgcggtgct tgctcgcgta aacccggtgg
    12121 cagaggagct gcgcttaagg tcgaaacttt ccccagaact atccgataca tatcagggcc
    12181 aacgtttaat gatgatggat gtattaatgc cgccaaaggc aaagttgtta ctctgcaaaa
    12241 actcacaatc aaccttgcgg gcttcatgca tgatgtaatc cagcgcgcca caattcgggt
    12301 ctggtttatt caggtttagc gtaggcgcaa accaaccttc gcgcatcatt tgcagactca
    12361 tccaggcttc gagcgcgcca caggcaccaa gcgtatgacc aaaataactt ttcagcgagg
    12421 agagcggcac attatcaccg taaatggttg ctgtcgccag actttctgcc atatcaccgc
    12481 gatcggtcgc cgtaccatgc gctgaaatat agccgatatc ttgtgcgctt aacccggcga
    12541 ttttcagcga ttgctccata caatactgca tggtttcgcg ctgcggctgg gtaatatgtg
    12601 ccgcgtcgca gttagtcgca aagccgacga tttcaccgta aatggtcgcg ccgcgcgctt
    12661 tggcatgctc cagttcttcg agaatgagcg tacctgcgcc ttcaccaatc accagcccgt
    12721 cgcggttttc atcgaacggt gaaggtgtgg ttttcggcgc atcattgtgc tggctggtgg
    12781 caaaaagggt atcaaacacc gctgcttctg aagggcataa ctcttcggca ccaccggcaa
    12841 ccatcaccgt ttgataaccg tggcgaatgg cttcccacgc ataaccgatc gcctggctgc
    12901 cggaggtaca ggcgctggag gttgggatca cccgcccgcg caggccgaaa aagaggccag
    12961 tattaacagc ggtggtgtgc ggcatcatct gcacataggt ggtgccagtg atgttgttcg
    13021 tgtgcttttc tgtcagcatg gtggcgaact cgcttaccgg accggtactg ccggtcgatg
    13081 aaccgtacgc aatgcccgtt tcaccgctgg ttaatatcgg atcgccaatc aaccctgcct
    13141 gctctaacgc cagctcgctg gcacgggtcg acatttgcga cacgcggccc atcgcacgga
    13201 tacgcttgcg ggtgtagtgc tccggtagcg tgaaatcatc aacaggcgcg ccaagcaggg
    13261 tgtgcagacc atcgtagacc tgccactccg gcattttgcg caccgcattt tcatacgcca
    13321 gtaagcgtgc agaaacgtcc tgccagtttt caccaaaggc agtcacgccg cccattcctg
    13381 taatcactac gcgacgtgtc ataacatccc tccgttgatg gaaataacct ggcgggtgac
    13441 gtatcccgca atatcagaca ttaaataact ggcaagcccg gcaacttctt ccgcctggcc
    13501 catgcgtttc atggggatca tcgacattgc ctctttcagg gcgctctctt ccatttcgat
    13561 catcccggta tcaataagcc ctggcgcgat gcagttgacg gtgattttgc gcttcgcgag
    13621 ttcgatagcc agtgcttttg ttgcgccgat aattccggct ttggctgcgc tgtaattcac
    13681 ctggccgcga ttgcccatta cgccggaaac tgaggacagc gtgatgatgc gcccgccctg
    13741 gcgtgtaccg atcatcggca taatgcacgg ctgaataacg ttgtagaaac tgtcgagatt
    13801 ggtgtggatc accgcatccc agtcgtcatc gcttaaagcc ggaaaagcgg catcgcgggc
    13861 gatcccggcg ttactaacca cgccgtacca ggcaccgtgt tgcgcgattt cgtgctccag
    13921 cacttcccga cactgttcgc ggtttgcgac gtcgaaactg agcaaacgcc cgttaccccc
    13981 gttggcgaca atggcattca gcgtttcctg agcgcctgtg gcatcacggt gatagtgaac
    14041 gccaatgtta aaaccgtctg ccgccagttg gcaggcgatg gcgcgaccaa tgcctttgct
    14101 ggcaccagta accagaactg aacgactcat caggacgctc cttgttgaaa cagggtggta
    14161 agttcttcct cggtcggctg gaaggtgtta atgcggcccg tcgcctcgcc gtcgttgatg
    14221 ctgcactcaa aactgccgaa gcgatcatcc tgcatcagca gtttgacggt aatggtcagc
    14281 gtctggcctg cgggcagaat gccagcggcg caaagcaatt cacgcgcgcc cagtaccatc
    14341 cctaactgaa tactgttttt gccctgctgg cggcgatgcc agccagacca gacgccgaca
    14401 gtttgcgcca tcagttcaag ggaaaaccag ccggggagat tgccgtccgg gtcgagaaac
    14461 ggtgccagta cgccactggg cgaaaccgtc acgcggcaca ccgcgctgtc atcgctgacg
    14521 ctcaccacgt cttccagcag taacatgggc gcgtcgtggg gcagataagc gccgggggat
    14581 aaatagtggc tcacgaaacc ctcccgagca gaatgctggc gttattgccg ccaaaggcga
    14641 aggaattgga caggatcacc ggacgtgcca gcggctgcgg tttttcgata atgccgcacg
    14701 gtggcagcgt gggatcgcgc ggcgataggc tgaagtcctg agcgggcagc ggtaaatcac
    14761 gctgtaaaat caacatgctg attgccgcct cggtaatgcc cgccgcgccc agcgtgtgtc
    14821 cggtcaaatg tttggtggaa ctgcaaggta cgcgttcgcc aaacagggcg ttaaccacca
    14881 tcgattcaat ctggtcgttg agttgtgtgg cggtaccgtg caggttgata tagccgacgt
    14941 catctggcgt aagctgcgca tcggtcagcg cctggttaat ggcgcggatc gccccttcgc
    15001 cctgcggatg cggggccgaa atatggtaag cgtcgctcga ttcaccaacg cccagtaacg
    15061 cgatgggctg cggttcacgg gttaataaca tcagtcccgc cccttcgcca atggtgatac
    15121 ctgcgcggtc acgaccaaac ggctgacata aagttggcga gagcgattcg aggctgtgaa
    15181 aaccgttaat cggcatccgg ctcagggtgt ctgcgccacc cacaatggca gcatcgacca
    15241 gccccgcctc gataagcctg cgcccgctga tcatcgctct ggcgctggag gagcaggagg
    15301 ttgaaagggt ataggccggg ccgtcgagca ccagccagtg gctgagaaaa cgcgacggat
    15361 cgccgagttc ctgttgcgcg tagtgccatg tggtgctcgg ctgtccgttg gtgcgcaggt
    15421 taacgtgcgt gtcgccctca tgcaggccgg aggtgctggt gccaagtaca atcgcgatgc
    15481 gctcgcgacc atatttcgct atagcgtcat caacttgcgg ctggatctgc gccagcgccg
    15541 ccagcaggat ctgattgttg cgcgagcggt gcgcagcgaa tttttccggg ataaccggaa
    15601 gctcgccatc cacgccagcc aggaccgcct gttgatgccc ctgcaaccag cctgcgcgcg
    15661 ggcgcatgcc gggagcgaca ccgcgagtca ggttggctgc gatatcgtca aggttatttc
    15721 ccagggcgtt gatcatgccg acggcggaaa tgtaaatcat attagtcacc taagtattga
    15781 atggtgatat ggtatttaaa gacatgctgc tcaatgctga ttggcacgcg tttgccttgg
    15841 cgattcatat agatgatttc cgtgaccagc ttgccgctgg cgttacgcag ctcgcgtttg
    15901 ttgccgttgt cgcgaagcgt ccagctagtg ggaagttgcg gttgccaggc gctaatcggc
    15961 cagtggctga gcattacgtc agccagcacc tgacttgcgg gcggcagctg cgggacgacg
    16021 atggattgct cggcgcgtag accttttgca tcgtaggtca ccagaaacag gcgaatgccg
    16081 accgacgaca gcccagcaag ggtgattttc tggtcgtcgg cgttaagcat cactagcagc
    16141 gactgggttt tgccgttgaa gctgccggtc aagagttgct gggaattgac ggcgggtgaa
    16201 atccccggcg caggcagcgt gatgcgtgtg ccaggttgca gccacgcctg cgggcggcct
    16261 tcctgttccg gttgcgagtg gctacagccg gtgaggataa gcgtagcggt cagggcgaac
    16321 gctcgccaaa atgtatgttt gatcattttt ttgttctctt tttatcgggc atagccagcg
    16381 gcgaaagcag gaaggcggtg aaaataccgc tcaccagcac aatgccaaaa ctgctgatgg
    16441 cctgggtggc gctgaatacc agcataccca gcgtcagcaa ggtggtgagc attgccagcg
    16501 tgatcgccag tagggaagtt aacggtgtac cgcgcggatt actgaaaaac agcgtgtagt
    16561 tgataccgat gccaagcacc agcaccagcg ccagcagcga aaagagattc accgcctgcc
    16621 cgctaatcgc cagcaccgcc agaccgcagc ccagcgaaag caccgaaggt acaaggctga
    16681 taagcccttt gcgccagccg aggcgagcta ttgcgccgca ggtaatcacc gccagcgcca
    16741 ccagtaacaa gccggttaag acataacggt aaagtgcgaa caactcatca aaggtacttt
    16801 tgcgatcaac ccaggcaatg ccgcaagggt aatagctggt aatttcctgc aacaacgcgc
    16861 tacttttaac cccttcaacc ggcaccagta cgccgctttc gccgttttcc agcgtcagcc
    16921 acagcagacg ccagccttca ctggcggggc ttgccagcca ttcatcaacc ttcaccggca
    16981 tggcgttgag atccgggttc actgtcgtca gcccggcatt ttgcagtgct tttgttactg
    17041 tcggggccgc tgttgtcagc agatgtaaat cttcctcctg ccgcgcaagg gagttcagcg
    17101 gaatggtgcg gtatttgctg ataagcccgt cttttttcgc cttctccagc gaggtggtaa
    17161 atttctccag tcgccgtaat gtttgctgtg gggagtcgcc gtaaaccacg aaccattttt
    17221 gatcgacgct ctgcccggtc agggcggtaa tggctttctc ctgcgccaga atatgctgcg
    17281 gtagcgcctg taactgcgag atatcgtcat cgacgcgtag cattgacatc cccgccagcg
    17341 aaaacagcgc cagcgcgacg ggcagacccc gcgacagttt tttattgcgc cgccacgctg
    17401 ccagccagcg tagcatcagc gccatcgccg gaaccgggcg caccggcaga ccacggcaca
    17461 gccacggatg ccagaacagg acggtcagac aagaggcgct caacccgacg gcggcaaaaa
    17521 tcgccatctg gcggataccg gggaaggggg cgagcatcat aatcagatat gccgccacgg
    17581 tggtgagcag cgccagtagc aaggcattgc gtactttcgc caggctttgc cacggcgaaa
    17641 cgtcgttgcc gtgcaccatc cgctcggtga gataatagag cgtgtagtca gcggaaatgc
    17701 cgataacgct catgctcatc accagcgtca tcaggtgtaa ttcaccgaaa atcaataacg
    17761 tggcgaccgt tcccgccagc gcgccgatgc cgatggaaat cacgcaaagc aacaatgggc
    17821 gcaaagagcg gaacaccgcc acaatcagca atatcacccc cagcagcgtt gccacgccca
    17881 gggttgagat atcctgcttc gcctgttgac tggcgtaatc gctgtaaaat accgtgccgc
    17941 gcgagagcaa ctgcgcctgt gggtaacgcg ttttcagatc cttttccagc gtattcaggg
    18001 tcgtgatgag ctggtgggtt tgctgcatat caaacgacga tcccgccagt tcgccgtgca
    18061 gcagatacca gtagttgccc tggggatcct gcgtcaccag ccagccgtcc atcagccgca
    18121 aacgctggcc gtttttcgcc atcgctagct gcgagccgcg cattaacatc agcggatcgt
    18181 tttgcagctc tttgccgctt accccggaga acgcggaata aagctgggag aggatccact
    18241 gcgcctgcgc ttcgccgccg ttttgcaggc gggcgcgggt gtcggggtca atcaggccgt
    18301 tgcggtgctg ccagaaaaac gctccccacg cctgctggct ggcggcatcc attggccctt
    18361 taatgtcgcc gagcgcagcg gatttttgca gcagcgtcag ccactcctga gcgaccctgg
    18421 gattagcctc tttaccgggg ctgaccagcc acaccagttg gcggtccaga cgctgcataa
    18481 agccgtcatt cagcgccgga ggaatatcgc ccatcgcctg tttgggtaac atagccagca
    18541 cgctactgtt cagtcgtgat tgtggcagca ggatcagcaa cgccgccgcc atgaccaggc
    18601 agactagccc ccataacagc gcggggcgtt tactgggtgg caaagcgttg gcgttcgtca
    18661 tcggtcagtt gcgctggcgt cagttgatgt tggataagag cgatatcggt gcgatcgccc
    18721 tgtttatcat taagctgaat gctctccaga taggtttgcc cggcgagatc gatggtgttg
    18781 aaaattttgt ccagcggcgt ggtggtcggc gtcaggcgca gcgtccagcg gccttcgcct
    18841 ttgtcggcaa attcaacgtg gaagttttgt tccagcactt tgcgatcggc ctggaacagc
    18901 gcgcgcagca ggtggttaaa ctgaaacatc tgcgggttat tttccgcagt gatgatttgt
    18961 ggcggctgac cgttgatcac ctgcaccatg cgtttatcgt ccagcaatag ctgcatggga
    19021 aatggtgagg tttgattcca caataacccc tggtcgcggg cgatcaacat ctggccctga
    19081 gatcgcagcg gctgcggcag atctttaatc gtccgggttt gatcgaaatg ggcgcggatc
    19141 accggctgtt cggtaaaacg ttgttgaaga tcgtccaggg tcagggcgct cacaaacggg
    19201 ctaatcagca gcgccagcag cggtaaaaat ttcatggttt gactcccatg cgttcaaaca
    19261 gaatatcggg gctgacaaag cacagttcgc ggctttgctc gtcgaccgcg acctgaatgg
    19321 tgtatccggt ggtggcgcgt ttgcccgttt cggcatcgaa aatctgatag ccaatccgca
    19381 gacggttttc gaactcttcg atatgcgcac gcacgcggat gcgttgctca aaggtgagag
    19441 cgtggcgata tttcacccgc gcatcgacca ctggccacaa ataaccggac tctttcatct
    19501 gccgatagcc gtaattgaac tggttgagca gcgcttcgcg ggccacttca aagtagcgaa
    19561 aatagttacc gtgccaggcc acgcccatca tatcgacgtc gtgaaaggga atggtcagct
    19621 cgacttcagc ggtaaatcgg ggatcgttaa gcacccttta ctccttgtcc tgaatttccg
    19681 gcagttgcca gaaatcgaaa aaattaaacc agtcgagggg cgactggagc gcgtaatgtt
    19741 ccagacgcgc ggcgtagtgg tcgatagcgt tttgcagcgc ctgttggcgt tctccacgcg
    19801 gcaacagcag cgggtcggca aaggtttcgc agtgaatatg cagcttgccg tgctggcgta
    19861 gggcgaaaat cagattcacc gggcagcgca aaatagaggc gagaataaac ggcccctgtg
    19921 ggaaaggcgc aggctggccc ataaacggac tccagcagac acgccagtcg ccgccgcgtt
    19981 gcgggttgac ggcgatgcgg tcaccgacaa tcgccaccca ttcgccgtta tccagcttct
    20041 ctttcagcag gatggcggtt tccgggccga tatctgttac cggcatcagg ttaatgcctg
    20101 cctgaggagc catctcctgc attatctgtt taaagcgttg ggcgttttcg ctaaacacca
    20161 gcgcgttaat ggttttgtag ccctgaattt ttgccagcgc ccggcacact tccacatcgc
    20221 caagatgcga tgccagcagc aatttgccgc gcggatcgcg gacgtcaagc gccgcttctg
    20281 cgcctggcgc aaacagcaca tcgcgcccca gttgtagctc gccgcgccag ctggcgattt
    20341 tgtcgagcat ggcgttaccg aaacgtagaa agtgctgata actgttgagt tttgccgcct
    20401 gtggttgatg ctggcgcaca cgggcgagcc agtcttgcga tgctttgcgc gcacgagcag
    20461 cggtgagcca gtagacgccc accaccgggt aaagcagcgc gctaaacgcc gttcttccca
    20521 gcagacgcca gaccagcagc atcaggcgca ttccccataa tcctttcact tcactctgcc
    20581 gcgcccagtg gctggaagag cggcgcatca gcagtgaagg aatacgcggc aacatgccga
    20641 aaaacagacg cgtgtgcatg agcgagatgc ggacgttatc tttcagggca tcaaaatgcg
    20701 agagcccgtc ctgcggataa gtgacgcggg ttggcacgaa ataactggta tttccctgcc
    20761 agtagaggcg caccatcact tcggtgtcga aatccatccg cttgccgatg gtggcgcgtt
    20821 ttgccagttg cagagttggc gcaaccggat aaacgcgaaa gccgcacatg ctgtctttca
    20881 gttgcaggga gagcgtttcg atccataccc agacgtgggt gacccagcgc ccgtacagcc
    20941 gtgagcgggg aatggagtca tcgtaaattg gctggccgga gatcagcacc gcaggatgct
    21001 gttcggcaag tgccagcagt ttagggatat cttcaatcgc gtgctgaccg tcagcatcca
    21061 cctgtaccgc atggctgaac cccgcctctg ccgctgcctg taagccacgc atcactgccg
    21121 cgcctttgcc tgcgttttct gccaggcgaa ttaaggtcac gccaggctgt tcggcaagca
    21181 gactgtccag ttgctgttgc gtggtggcat cgctgccgtc atccaccaca atacagggca
    21241 gattaaatgg cttaagacgc gccagcacgc ccggcatcat cgcgccgtgg ttgtagcagg
    21301 ggatcaacac gcagggagaa aagtttaccg acataggcgg atcttcccgc tactggcggt
    21361 gtggcgggcg tcaccgtcgt gacgctgata gctaaagctc agaatctggc gttcttcctg
    21421 ccagttaagc gtgagcgtga cacggttttc cggcagcaac ggagactgga attttatgtt
    21481 ctgaatggag tgaaaacgcc agccaggcgc gagcagagtg agcgcgtagc tcatcgccca
    21541 gtcgatttgc gccacgccag ggagtaatgg ttgcacggca aaatgaccgc gaaaccagaa
    21601 cagatccgcc ctgagatgca gaacaatttc aaggtgattc gcttgtgcct gatggcgctc
    21661 gatttcatgg ggcttcatga aataactcct gtaattgcgc atagacacgc ttgttcatac
    21721 tgtttactgg catttcatca ataacgcgcc agtaacgcgg aatagcaact ggctccagcg
    21781 tcgggcgcag taatcgccgc caggtcattt cctggctatg cccgctgccg ttctgccatt
    21841 gcagatgagc ttcgtcatcc agcaccagca aaacgccgat gctctgacgc ccgccgcgtg
    21901 taacgggaac agccgccgcc tcgcgaatgc cgtccagcgc cagcagacgt tgttccactt
    21961 cctggagtga aatgcgtttt tcttcaatct taacgatgcg tccgcgacgc cccatcagat
    22021 ggaactgacc gtcctggcta aactgcaaga gatcgtcgag taacaggccg ttatcttccg
    22081 ccatcagcgg tgaaaagaga cgaaaagaat caccttcggc ctggaactgc ataccaggga
    22141 agggtagcca gcggcgttgc tcttgttcgc ggtaacgcca ggcgatgaca ccggtttccg
    22201 tgctgccgta gatttcatca ggccagacgc gcagccagct cgcggtatgt tgtacatcct
    22261 gccacggtag ctcaccgcca gcagacagaa gcacctgaac gggcggcgga gaaagctggg
    22321 tatccaggcg cttcaaaaat gccgggctgc tgatgaagat atagcgatgc tgatgactca
    22381 acgcggcaaa ctgttcgaca taccagagca tggcggcgtg cagcggtaat cccaacacca
    22441 tgggcaggaa aacgcgaaaa gtcaggccgt acaaatgctg cggcaatacg gaaccgacga
    22501 cacgacatcc ggcaagacgt gcgcctaatc gttcagccag cagttctgct tcacgatcca
    22561 gcaaatgaac aggtttaatc acccgtttcg gctgtcccgt ggaaccggag gtaaacagct
    22621 cgataaaagc ctctggcgct atagcggcaa aggtgaagga ttgtgtcgcg atttgtgggg
    22681 aacttgctac caacagcaat gaaccttgcc agttaagttc gctgtcgcta agcacaccat
    22741 caaaaagttc acgttgctcg ttaagctgga taacacggtt atgccccgga agcaccggcg
    22801 tttttccggc gtgtagcgtg gcgagcaggg caacgataaa cagatagctg ttctcaaagc
    22861 acagcgccca gcgttcaccc ggctgttgct gcaagcggca gataagttgc gcgacatcgt
    22921 gacgcaaatc gcccagcgtc caggtacttt cgttcagcca cgcaatgggt gtgtcatcag
    22981 ggcgtggcgc agtcagccag cgtgcaagcg gtaaggtctg cttcatgcgc gatctctttt
    23041 cataacctgg cggcgcacca gccattcacc agccattaac gtccccatca acaaataagc
    23101 aatcatgccg ttccaggcgg tccataacga catatcggca taaagcgcgg taaagagggc
    23161 aattccaccg ttaatgatga aaaagccgca ccagatttgc gtgacgtggc gggtatagcg
    23221 cacgcccttt tccgggaggt ctggctcctg caaccgcgcc agccgctcaa ctatcggcat
    23281 tgccgaccac agcgaaccgc caaaaaccgc cagcatgacc gcgttaacca ctactgggta
    23341 aaacagcagt agctggtgcg ttttcaacag aaagctggcg acacacaatg cgatgcccac
    23401 gacagcgacg agttgcgtga cggcttgtaa aggccccgcc tggcgacggg tctggcgcag
    23461 gcgcaacaaa agcagcaacg ccattagcgg caacagccag tgcaggccgt tatgcgctaa
    23521 gccaaaccaa atcacaaacg gccaggcgag cagcaataat ccagtcaaca gcttaatcat
    23581 cggtagcgag cgaatgcccg acatcagatc aggcttcttg tagcaggcgt tctacagcct
    23641 cgacgacatc ctggactgta cgcactgctt taaactcttc cggcttgatt ttcttaccgg
    23701 ttttcttttg caggtgcaca atcatgtcaa cggcgtcgat gctgtccagt tccagatctt
    23761 cgtacagtcg cgcttcaggt ttgatgtcct gcggatcgat ttcaaacagc ttaaccagca
    23821 gggctgagac ttcctgatag acggtttgtt gttctgtcat gatggctttc ctcaggcacg
    23881 ttgcgcagca atgaaagagg ccagagtggc gacggagaaa aagtgctgac gcatctcttc
    23941 gctttccgca gagagcacga tgccgtactc attttttact gccagtccca gttccagggc
    24001 atcgatagag tccagcccta aaccgtcgcc gaacagcggc gcatcggtct cgatatcatc
    24061 aggagtcaac tcgtccagat tgagggtgct gatgataagg tttttaattt ccagataaag
    24121 cgcttgcatt atttattcct gagagaggta aacaaccttg ttgtaattga agcaaaagat
    24181 gccggtttaa ctgccttgct gccagcgccg gttcttgtga agttgcatcg taaaatttat
    24241 ggatttcaac ccgaccatgg actgcaaccg taaaaaatgg ccggtcaggt ggaacgtcat
    24301 accatttact ttgtttatcc agcagatgct cgctacagcc gattgtcacg atgcgtaaat
    24361 cgctggcgca gcgcacggca atattcgccg ccccgcgctg cagcgtcatt ttttcaccgg
    24421 gcttcgttcg tgtgccttcg gggaaaatca ggatagtgtc accctgtgcc agtcgctgtt
    24481 ggcagcgggg taacagagtt tcagcttcgc tgttgattaa ataatctgcc gcccgcacga
    24541 cgccgccaag aaaaggattt tttaacagcg cgcttttcac caggcagtca gtttctggca
    24601 tgactgacgc cagcagcacg taatcgatca gcgtgggatg attggctacg acgaggcaac
    24661 cgcgttcctg atgtaagatt tcagcgccat caatacgata atccaacacg ccaagccctt
    24721 tcgcgacagt caaaaacagg cgaaaactgg cggcaatgct gcgacgcgcc aaacggcggc
    24781 ggcgggaagt atcccacacc aaaactaaca atatgttaaa ccacacgaca gaaagcagca
    24841 ggccgccgag accaaacagg gcaaaacata acccggtcat gaccagtcgc catgcccatt
    24901 ctaggcgcga catcagctta ctcatctgcg gctccagcgc cattgcacgc gctcgccaga
    24961 cagtgaaaat gtgtctgcat tttgtaaata gtgctgtaaa aacaacatgc tttgcggcag
    25021 tgttgtttca ttgcccgcaa tggctggctg tgtttcacat tgccagtcat cgccagcttc
    25081 aatcaccagc gcgacggcgt acggccaggt cggcattttt gcgggcagtt gcgggtgata
    25141 aaactcaggt agaaagccat caaaatcgac catcaacacg cgctggtatc ccgcttgtag
    25201 caggctaagc acttcacaca aaccttgctg gaaactatcg cgaccagccg aaagcgatga
    25261 cgaaacgatc ggctgtttgg ccgcaatggt caggttaccc acggaagagt tgtgtacgga
    25321 gagcgcgaag tccgtcggag aaagcgcctg ttcggttgcc agtgcatgaa caatgcggta
    25381 attgcgctcc agttcgccat gacgactggt atacaaaacg gcgtctggtt gatggcggcg
    25441 tagcattgcc agaccgcatt caacagccag tttgctcccg gagctgaggc ggcgggcggt
    25501 catcattggc agttcactta attttgccaa cggcgcggca gggtcaatcg cccacggctg
    25561 gcgcgaccat gcttgccatt gctgcgcttc gctaagtcca ggcgccagtg cctgccagtc
    25621 ggtaatattt aatgtaaatt tcatcaacgc gcgcccgcgg taaaccaaaa ttattaagtg
    25681 acgattattt cttttggcat atcaaaagtg tatgcccaac gcctaaatta tcgtggcgct
    25741 ctgctacctg gaatcctgct ttattcagat agtcgtaaaa tttctctgca ctatagaaac
    25801 ggctgttacc gtttgccatg caggtaaaat aaagtgagga cgcgttcagg ctaaacgatg
    25861 ctgcttcaaa ccgctgcgca tcccagaaca actccatgat gcaaactttt gcgccaggct
    25921 tcattacgct ggcaattttg ctgagtatgc taattatttg ttcgggtgaa aaacagtcta
    25981 aaaattggct catccaccag atatcagcct cgcccggtaa tggggcatcg cttagcatat
    26041 ccaccgcatg gaaatctatg cgatcagaaa aacctgcatt cgcgatattt tctttcgcta
    26101 gtacgatttg ttgtggcaga tctaataacg taacggcgat gttttcattg tatttgcagc
    26161 aacgcaaagc ccacttaccc gtattgccgc ctacatcgta caatgttgtg gggttgttgg
    26221 cgaatacata aggcagtgcg gcgttaaatg caccatctga atagtaatga tcaaacgcaa
    26281 accagctttc acgagcggcg tctggtaatt gcgaaagtgc cggatatatc gtcgaccagt
    26341 cgccaaatac ttttaacccg gaaggttttc cttcattgag tgaatcggcg aggaagaaga
    26401 gcccctggta acagacatct tgggtgaaat ccatattcac gcgagtcata gtgtcatgca
    26461 ggaggtaatg accgattttc gccaggtaat aaattccctc tttacaggta ataattcgcc
    26521 cgcttaagcc catatctaac agaacgctgg cagcatattc gttgatatgg ctatgttcgg
    26581 tgatatcagc cagcgttgcg ccatgtttgc cttgccgatc cagatatgaa agtacacccg
    26641 cattacgtag gcagagtgct gtttgaaaaa gcataggggc aaaggcaatg cgttgagctt
    26701 cggtaatcgc atccagtgcg ctaagagaat cctgttcgta catgttgggc acaacctgtt
    26761 aagagggaat taaattatta tcggaccgca acagcccgac tttaggaaga attataaatc
    26821 tgcgcccgct gacaaattca gttgaatatc tttgtcgaga ttgcggaccc agcgattgta
    26881 gtttttatga atttgtccgc ctgacgcttg taggttctgg ctattttcat aattgatatc
    26941 gtaactggat gccgtgtagt taatgcgtat ttccgctgaa tgatcgcgtg actgttgacg
    27001 tgctttaatt atgccttgtc cggcttcgga cataatccat ttgcgttcaa cgccagcttt
    27061 cagaatcgct tttttcactt gttcctgggt atgaccagcg ctgacggttg agtgaacctg
    27121 atcaattggc gcagtacgcg cgcaacccgc taaagcaccc acaacagcaa tagccgcaaa
    27181 ccatttcatg taatgtctca ttagaacccc attaaagtat tgtttttacg agtaaagcaa
    27241 gatttaactg taattgctgg catcactatt ctttatttag agaaaaaatt acttgtgaga
    27301 taagtcgtta agtgcacatc agagtaaaaa tctctgaaat gactaaatta ctgccagcgg
    27361 ggaccaacta taagtgagtg agaaaaatga ggcaataaaa ataacacaat gttatcagtg
    27421 cattctttga gaaggattta atcgc
//
LOCUS       DAZFJL010000060        27289 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFJL010000060 DAZFJL010000000
VERSION     DAZFJL010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.060"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(51..449)
                     /gene="mutT"
                     /locus_tag="ACHJ8C_002413"
     CDS             complement(51..449)
                     /gene="mutT"
                     /locus_tag="ACHJ8C_002413"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO5552258.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKVEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADGFPPANEPVIAKLKRVYAG"
     gene            complement(509..3214)
                     /gene="secA"
                     /locus_tag="ACHJ8C_002414"
     CDS             complement(509..3214)
                     /gene="secA"
                     /locus_tag="ACHJ8C_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO5552259.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(3276..3788)
                     /gene="secM"
                     /locus_tag="ACHJ8C_002415"
     CDS             complement(3276..3788)
                     /gene="secM"
                     /locus_tag="ACHJ8C_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO5552260.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(4019..4936)
                     /gene="lpxC"
                     /locus_tag="ACHJ8C_002416"
     CDS             complement(4019..4936)
                     /gene="lpxC"
                     /locus_tag="ACHJ8C_002416"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO5552261.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(5037..6188)
                     /gene="ftsZ"
                     /locus_tag="ACHJ8C_002417"
     CDS             complement(5037..6188)
                     /gene="ftsZ"
                     /locus_tag="ACHJ8C_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO5552262.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(6249..7511)
                     /gene="ftsA"
                     /locus_tag="ACHJ8C_002418"
     CDS             complement(6249..7511)
                     /gene="ftsA"
                     /locus_tag="ACHJ8C_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO5552263.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(7508..8338)
                     /gene="ftsQ"
                     /locus_tag="ACHJ8C_002419"
     CDS             complement(7508..8338)
                     /gene="ftsQ"
                     /locus_tag="ACHJ8C_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO5552264.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(8340..9260)
                     /gene="ddlB"
                     /locus_tag="ACHJ8C_002420"
     CDS             complement(8340..9260)
                     /gene="ddlB"
                     /locus_tag="ACHJ8C_002420"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO5552265.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLNDKQLAEISSLGLPVIVKPSREGSSVGMSKVVAE
                     NTLQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(9253..10728)
                     /gene="murC"
                     /locus_tag="ACHJ8C_002421"
     CDS             complement(9253..10728)
                     /gene="murC"
                     /locus_tag="ACHJ8C_002421"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO5552266.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(10782..11849)
                     /gene="murG"
                     /locus_tag="ACHJ8C_002422"
     CDS             complement(10782..11849)
                     /gene="murG"
                     /locus_tag="ACHJ8C_002422"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO5552267.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQSQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(11846..13093)
                     /gene="ftsW"
                     /locus_tag="ACHJ8C_002423"
     CDS             complement(11846..13093)
                     /gene="ftsW"
                     /locus_tag="ACHJ8C_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO5552268.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTNSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRR
                     LPMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCY
                     IANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGA
                     KLWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGE
                     LWGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKA
                     LEIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAI
                     MMLLRIDYETRLEKAQAFVRGSR"
     gene            complement(13093..14409)
                     /gene="murD"
                     /locus_tag="ACHJ8C_002424"
     CDS             complement(13093..14409)
                     /gene="murD"
                     /locus_tag="ACHJ8C_002424"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO5552269.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVCWVNDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLATRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(14412..15494)
                     /gene="mraY"
                     /locus_tag="ACHJ8C_002425"
     CDS             complement(14412..15494)
                     /gene="mraY"
                     /locus_tag="ACHJ8C_002425"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO5552270.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(15488..16846)
                     /gene="murF"
                     /locus_tag="ACHJ8C_002426"
     CDS             complement(15488..16846)
                     /gene="murF"
                     /locus_tag="ACHJ8C_002426"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO5552271.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFAGQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFLIKLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDCVLSVGK
                     QSHAISTASGVGEHFSDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(16843..18330)
                     /gene="murE"
                     /locus_tag="ACHJ8C_002427"
     CDS             complement(16843..18330)
                     /gene="murE"
                     /locus_tag="ACHJ8C_002427"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO5552272.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFTASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSAHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TDVNYHDSGATIRFSSSWGDGEIESRLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGYAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(18317..20083)
                     /gene="ftsI"
                     /locus_tag="ACHJ8C_002428"
     CDS             complement(18317..20083)
                     /gene="ftsI"
                     /locus_tag="ACHJ8C_002428"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO5552273.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLTLSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(20099..20464)
                     /gene="ftsL"
                     /locus_tag="ACHJ8C_002429"
     CDS             complement(20099..20464)
                     /gene="ftsL"
                     /locus_tag="ACHJ8C_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO5552274.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(20461..21402)
                     /gene="rsmH"
                     /locus_tag="ACHJ8C_002430"
     CDS             complement(20461..21402)
                     /gene="rsmH"
                     /locus_tag="ACHJ8C_002430"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO5552275.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(21404..21862)
                     /gene="mraZ"
                     /locus_tag="ACHJ8C_002431"
     CDS             complement(21404..21862)
                     /gene="mraZ"
                     /locus_tag="ACHJ8C_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO5552276.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSTNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(22464..23468)
                     /gene="cra"
                     /locus_tag="ACHJ8C_002432"
     CDS             complement(22464..23468)
                     /gene="cra"
                     /locus_tag="ACHJ8C_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO5552277.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(23550..23636)
                     /locus_tag="ACHJ8C_002433"
     CDS             complement(23550..23636)
                     /locus_tag="ACHJ8C_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO5552278.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(23648..24139)
                     /gene="ilvN"
                     /locus_tag="ACHJ8C_002434"
     CDS             complement(23648..24139)
                     /gene="ilvN"
                     /locus_tag="ACHJ8C_002434"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO5552279.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(24142..25866)
                     /gene="ilvI"
                     /locus_tag="ACHJ8C_002435"
     CDS             complement(24142..25866)
                     /gene="ilvI"
                     /locus_tag="ACHJ8C_002435"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO5552280.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHLQLKEAVEVLNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTHGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            25985..26023
                     /locus_tag="ACHJ8C_002436"
                     /pseudo
     CDS             25985..26023
                     /locus_tag="ACHJ8C_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26184..27128)
                     /gene="leuO"
                     /locus_tag="ACHJ8C_002437"
     CDS             complement(26184..27128)
                     /gene="leuO"
                     /locus_tag="ACHJ8C_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO5552281.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     ALGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQTWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         6560 a         7759 c         6531 g         6439 t
ORIGIN      
        1 ccagcgtcgc atcaggcatc atatgcaaat gtcggatgca gcgtgacgcc ttatccggca
       61 tacacacgct taagtttcgc aattaccggt tcattggctg gcggaaaacc atcggcatta
      121 agaccgacca gcgacatcca ctcaccgggt tgcccttctt taccccacgg ctccccttcc
      181 cagctttcga ccagccagaa ccacagagtt atatgcctgt ccgggaattc atattccagt
      241 ttttcaaata gcgaaaaatg ttgtggggta atcccgactt cttcctgaag ttcacgcacc
      301 accgcctgtt ccggcgtttc gcccatttca actttaccgc cgggaaactc cagtttattc
      361 gccatgtgcg catctgctgc gcgacgcgtt ataaaaattt cattgttctc gttgcgaata
      421 atacctaccg caatttgcag ctttttcatt gtcttaaacc tataaaaaag gcgcagaatc
      481 ctgcgccttt tacttcaact gttagctttt attgcaggcg gccatggcac tgcttgtatt
      541 ttttaccaga accacacggg caaggatcgt tacgtcctac tttgcgttca ccggtttgcg
      601 ccgccagtgc agctgcggca gcagagtcgt catcctgatg gctaagctgc tgcatttgcg
      661 ctaaacgctc ggcttccata cgacgctgtt gttccagctc ctcaacctct tcaggcatac
      721 gtacctgaac tttgctcagc gtgctgataa cttcatattt taacgactcc agcatcgctg
      781 caaacatgga gaacgattca cgtttgtatt cctgcttcgg atctttctgt gcatagccac
      841 gcaggtggat accctgacgc agatagtcca tcgcagccag gtgctctttc cacagagagt
      901 caagcgtttg cagcatgacg cctttctcga agtgacgcat catctcagca ccaaccactt
      961 cttctttacg ctgatacact tcgatggact gcgccagaat gcgctcacgc agcgtctctt
     1021 catgcagttc tggttcttta tccagccact cggcaattgg taaatcgagg tcgaaatcgt
     1081 tcttcagacg ttcctgcagc cccggaatat cccacatttc ttccagcgac tgtggcggaa
     1141 tgtaggcatc aatggtcgct ttgaacacat cttcacgaat gctgttgatg gtttcgctca
     1201 catcgctgac atccagcagt tcgttacgct gggagtaaat ggcgcgacgc tgatcgttag
     1261 ccacgtcatc atattccagc agttgcttac gaatgtcgaa gttacggctt tcaactttac
     1321 gctgggcgtt ggcaatcgct ttggtcaccc acgggtgctc aatggcttcg cctggcttca
     1381 tacccagttt acgcatcatg ccggatactc ggtcggaagc aaaaatacgc atcagcgcat
     1441 cttccatcga caggtagaaa cgggaagaac cagcatcccc ctgacgacca gaacgaccgc
     1501 gcagctggtt atcgatacga cgggattcgt gacgttcagt accgatgata tgcaggccac
     1561 ctgctgccag taccgcatcg tgacgtacct gccagtcggc tttaattttt tcaatttgct
     1621 ctgcagtcgg attttccagc gcggcaactt ctgcctgcca gctaccaccg agcacaatat
     1681 cggtaccacg acccgccatg ttggtcgcga tagtcaccgc agccgggtaa cctgcctgag
     1741 caacaatcgc cgcttcgttg gcatggaatt tggcgttcag gacgttgtgc ttaataccgg
     1801 ctttggtcag ttcatttgac accagctccg atttttcgat ggagattgta cccaccagca
     1861 ccggctggcc tttcgcagta cgttctttga tatcttcaat gatcgcctga attttttccg
     1921 cttcagtcat gtagaccagg tccggcagat ctttacgaat cattgggcgg ttggtcggaa
     1981 caacgacagt atccagctta tagatggagc tgaattcgaa agcttcggta tcagcagtac
     2041 cagtcatccc cgccagtttt tcatacagac ggaagtagtt ctggaaggtg atcgaagcca
     2101 gcgtttggtt ttcgttctgg atctgcacac cttctttcgc ttccacagcc tggtgcagac
     2161 catcggacca gcgacggccc tgcatggtac gaccggtgtg ttcgtcaacg atgataactt
     2221 caccatcttt aacgatgtag tcgacgtcac gggtaaacag cgcatgagcg cgcagcgccg
     2281 ccgttacgtg gtgcatcagc atgatgttgg ccggagagta cagagactct ccttcatcca
     2341 tgatgccttc tttaaccagc agttcttcaa tcaggaccag accacgttcg gtcaggttca
     2401 cctggcgaga tttttcatcc accgagaagt ggccttcgcc ctggaaggtt tcggagtctt
     2461 ctttttcctg acggatcagg tgcggaataa ttttattcac gcgtttatac atttccgagc
     2521 tgtcttctgc cgggccggaa atgatcagcg gtgtacgcgc ttcatcgatc aggatggagt
     2581 ccacttcgtc caccagcgca tagtgcagtt tacgctgtac acgttcttca ggactgaacg
     2641 ccatgttgtc acgcaggtag tcaaagccgt attcgttgtt cgtaccgtaa gtgatgtcag
     2701 cagcgtaggc ttcacgcttt gccggtgctg gcatgcccgg caggttgata ccgacagtca
     2761 ggccaaggaa ttcaaacagc ggacggttgt tttcggcgtc acgttgcgcc aggtagtcgt
     2821 tgacggtaac tacgtgcacg cctttaccgg ttagtgcgtt caggtaagca ggcagcgttg
     2881 cggtcagggt tttaccttca ccggtacgca tttcggcgat gcagcgttcg ttaagaacca
     2941 taccgccgag taactgaacg tcgaagtgac gcatgccaaa gacgcgctta cttgcctcac
     3001 gcaccacggc gaaagcttcc gggatcagat tttccagcac ttcgcctttt tccagacgcg
     3061 cacgaaactc tgcggttttc cctttcagtt cttcgtcgga gagtttttcc atctccggtt
     3121 ccatggcatt gatgatgttg accactttgc gcatccggcg cagggtgcga tcgttacgac
     3181 taccgaaaac tttagttaat aatttgatta gcataataaa atctcaaacg ccccgcgttg
     3241 cggagttaat aaaatgaagt aaaggtttat tgttgttagc tgaggcgttg agggccagca
     3301 cggatgcctt gcgcctggct tatccagacg ggcgtgctga attttgcttg tggggtaaaa
     3361 tgcgcataat caatgcgata acccttttca gacggcgtgc cttcctgggt cagaagcgcg
     3421 ctgagcgtat ccagtaatgc aagatgttgc gcctgaagag gcaaagattc ttcagcaacg
     3481 ggcagcgttt gcggtgccat tgcgaaggaa agatggcgga ttaccgtgcg aatggcatgt
     3541 tgatgccagt aatcaacgga atagttcgaa ttcgggcggc gcgtgttggc ttccagcaag
     3601 gccaattgac caaagttaac tttggctgaa ggctcgtggt tgcgggttgt cgcttttgcg
     3661 ggcgcgtttg gttcggcggc attgctgagt gcaggcaaac ctaaactcgc cgcgaccatc
     3721 cctaataaga gatgcggcca gaagtagcgt ttaccaaact gtcgccagcg cgtcagtatt
     3781 ccactcacgt tattgccatc ccgatcgtat tcaaacgtat ttagcgcaca aatcttatca
     3841 ttaaagcctg aagtccacag cacgaatgaa gataagttgt gcgtaaaagt gcttaagaaa
     3901 acagcgttcg caccgttttt tatagctctt ataaaattca aaaaaaaacg actggttcac
     3961 ctggccggag agagtgccag atttgccagt cgaattttat acgacagtat aaatgtcgtt
     4021 atgccagtac ggctgaaggc gctttgaagg ccaacggcag ttctgcgtcg tcctggaagg
     4081 tcacatattc ccaggcttcc tgttttgcaa ggacagcctg cagcagtttg ttattcagtg
     4141 catgaccaga tttataagcg gtaaatgcac caataatatt gtgaccacac atgaacaagt
     4201 caccgatcgc atcgagcatt ttgtgacgca caaattcgtc ttcaaaacgc aggccgtctt
     4261 cgttcagtac gcgataatcg tcaacaacga tggcacaatc gaagctgccg cccaggcaca
     4321 aaccacggga ctgcagatat tcgatatcac gcatgaaacc gaacgtacgc gcacggctga
     4381 tctggcgcat aaacgcatca gcggagaagt tcatcgcata gcgctggttg ctggaatcaa
     4441 tagccggatg gttaaaatcg atggtgaaat ccagagaaaa accattgtac ggcttaaatt
     4501 cagcccactt atcgccatct tcgacacgaa cagtctcttt aatgcgaaca aatttcttgg
     4561 cgcagttcaa ctcgtcgata ccggcgtcaa gcagcaggta tacaaacgga gcggcgctgc
     4621 cgtccatgat cgggatttcc ggcgcgttaa cttcgataac aatgttatcg atgcccaggc
     4681 ccgcgagagc agcattgagg tgctctacgg ttgaaatccg cacatcatgc tcgttgacca
     4741 gacacgtaca gagcatggta tcacgcacag atttggcatc ggccgggaaa tctaccggtg
     4801 gattcaagtc ggtgcgacga tagatgaccc cggtgttggc cggcgcaggg cgtaacgtca
     4861 gggtgacttt cttgccggta tgtaaaccga cacccgtcgc ctgaacgata cgtttaagtg
     4921 tcctttgttt gatcatcgta ttatctcgcc aaattaccta tccaaccgaa gtgtactata
     4981 cattcggcgg gccagtttag cacaaagagc ctcgaaaccc aaattccagt caattcttaa
     5041 tcagcttgct tacgcaggaa tgctgggata tccagataat ccggctcttt cgcagtttgc
     5101 ggcgcattgt cattcacgac tttagcaacc ggcttctgct cctgggtcag cggagccata
     5161 ccgtgctgct ggtagcgatc catcactggc tgctgaacct gcttattggt caccagagtg
     5221 atttcaggac gtttgtccat gccgatacct gtcgcaacaa cagttacgcg cagctcgtca
     5281 ttcatatccg ggtcaagaga agtaccgata accacggtcg cgttgtcgga agcaaatgca
     5341 cggatggtgt tacctaccgt ttcgaactca tccagacgca ggtcgaagcc cgctgtgatg
     5401 ttaaccagca cgccgcgtgc gccagacagg tcgatatctt ccagcagcgg agaagagata
     5461 gccatttcag cagcttcttc cgcacgatct tcaccgctcg ccacaccaga acccatcatt
     5521 gcgtagccca tttcagacat tacggtgcgt acgtctgcaa agtccacgtt catcaaaccc
     5581 ggacgagtaa tcagttcggc gataccttgc acagcgcctt tcagtacatc gttcgctgcg
     5641 ccaaacgcat ccagcaggga gatacctcgg cccagaactt tcagcagttt gtcgttcggg
     5701 atagtgatca gagagtccac atgcttggac agttcagtga tcccctgctc cgcgaatgcc
     5761 atacgcttct tgccttcaaa gttgaaaggc ttagtgacga cagcaacggt caggataccc
     5821 aaatcttttg ccacttcagc gacgactggt gctgcacctg taccggtacc accacccata
     5881 cccgcagcaa taaagaccat gtctgcacct tccagcgccg cacgcagtgc atcgcgatcc
     5941 tcatcagccg cattgcggcc aacttctgga ttagcgccag cgcccagtcc tttggtgata
     6001 ccgctaccga tttgaatcgt ctgtccaacc gctgttttac gcagcgcttg tgcatcggta
     6061 tttaccgcga agaattcaac accttcaatg cgctcacgca ccatgtgttc aacagcatta
     6121 ccgccgccgc cgccgacgcc gatgacttta atcaccgcgt cattggtaag ttccattggt
     6181 tcaaacatag tttctctccg atttgtgcct gtcgcctgag gccgtaatca tcgtcggcct
     6241 cataaaaatt aaaactcttt tcgcagccaa ctattgagtc gcttgatcca cgagccaact
     6301 gatgctgtaa cacgtttttc tacttcagct tcaccgttaa gatgtgactc tttcccatag
     6361 tgaagcaatc ccaccgccgt cgaataatac ggctcctgag cataatccgt taaaccggta
     6421 atgttcagcg gcgcgccgat acgcacttgc gtatgaaaca cgcgctgagc acaggctgca
     6481 agaccttcga tctgcgctgc gccaccggtt aatacaatgc ctgccgccag gtgatgttta
     6541 accccttgtt ggcgaagctt ttcctgcaac tgcaatatct cttcgttgac cagattgagc
     6601 agctcggtat agcgcggctc gatcacctct gccagggtct gacgttgtag actccgcggc
     6661 ggacgaccgc ctacgctcgg cacttccacg ctctcatctt ttccaacgat ggagcccagc
     6721 gcacaaccgt ggcgaacttt aatcgcttcg gcgtcacttg gcggtgtgcc aaaggcgtaa
     6781 gcgatatcac tggtcacgac attgccagca taaggaatta ccttcgtgtg gcgcaatgcc
     6841 ccaccggtat aaacggcgat atccattgta ccaccaccga tatcaacgac gcagacaccc
     6901 agttcacgtt catcttccgt caataccgaa taacttgatg ccagaccggc aaatatcagt
     6961 tggtcaactt tcagcccaca acgttcaacc gctttgacga tgtttttcgc catatcattg
     7021 tgacatgtga tcaggtgcac ttttgcctgc atccgcacgc ccgaaagccc taccggattc
     7081 ttgatccctt cctgatagtc aatcgcatac tcttgcggga tcacatgcaa cacacgatgc
     7141 tcatcgcgca cacgcaccga tttcgcggta tggacgacgt tttccacatc ttcttgcgtc
     7201 acttcttctt cagaaatagg caccatacca atttcattct ggcagctgat gtgcttacca
     7261 gaaagcgcca gatataccga agagatctga caatctgcca tcaattctgc ctggtcaatg
     7321 gcgcgttgta cgcacttgac cacggattcg aggtcgttca ccccaccttt atccatacca
     7381 cgcgacgggc aactacccac gccaatgata ttgaccatac cgtcgggcag aacttcccct
     7441 actaaagcgg caaccttcgc ggtaccaatc tccagtccta ctaccagttt tctgtccgtc
     7501 gccttgatca ttgttgttct gcctgtgcct gattttgttg ctgagtagat tcctctggcg
     7561 gcaagggcgc ccagcctact gccgctccag agtcataacg caaatcaacg tagctaatcc
     7621 gtttgccatc ggtttgcgcc tgctgctgta aaaccggata aagttctaca aagcgagcca
     7681 aacgtttcat cgtatcgccc cgaccaagat tgagcttaat atcgttattc agcgtcaact
     7741 gccaggaacg tcgcgcggtc atcgccgctt ccttcagagt aaatctgtcc tttgccagca
     7801 tctgccccat ttcgcgatag ccctgcaaca cttcattggc gctgccttcc gggccataca
     7861 gcattggaag cacctgcttg ctggtgcgat ctggcggcac gctgaaggtg tttccttccg
     7921 cgtctaccat atgttgatca ttccaccgcg caatcggcac atattcaacc agatgaatct
     7981 tcaattcatc aggccactgc tttctgacgc tcacctgctt aatccacggc aggcgttgtt
     8041 ctatttgcgt ctggatgatg ttgacatcct gggtcataaa ggtacctggc tcacccaatg
     8101 ccaggatcga ctgccggata tcgtcattac gcgtgtaatg gcgttcaccg gtcaacacca
     8161 gctttgagag cggcaggcgt tgcgcatctt ccatccagcc caacacgacc cagccgctca
     8221 ccaacactgt cgttaaaacg gtcagcagga aaaggatccc cgccagacgc gttccattat
     8281 tgcggcgaga agaaacctct tcttcgctgt ttcgcgtgtt cagagcagcc tgcgacatat
     8341 tagtccgcca gttccagaat tcgtactacc aactgcgaga agctcatacc tgcctgacgt
     8401 gccgccatcg gcaccaggct gtggctggtc atacccggtg aggtattggc ttccagcaga
     8461 taaaactgtc catcgctgtc cagcataacg tcaatacgtc cccatccttt gcaacctaac
     8521 gtcgtccatg ctttcagcac taatgcctgc aaattggcct cttgtgacgc ttccagacct
     8581 gcggggcaga aatactgtgt ctcatcagag agatacttcg cctcataatc atagaaggtt
     8641 ccggacggtt gaatacgtat tgacggtaaa atttcttcac cgagtatcgc aaccgtgaac
     8701 tccggcccac ttagccattt ttcaatcaat acttcttcat cgtgctgaaa tgccaatctt
     8761 aatgcatctt gtagagtatt ttctgctact acttttgaca ttcccacact ggaaccttcg
     8821 cggctcggct taacgataac cggcaaaccc agagaagaaa tttctgctaa ctgcttatcg
     8881 ttaaggcctt tttcaaactc tgcgcgggtt aacgctaccc acggcgcgac cggtaaaccg
     8941 gcaccctgcc acagaagttt gctgcgtagt ttatccattg aaagcgcaga tgccatcact
     9001 ccgctgccgg tataaggcaa gcccatcagc tcgagcatgc cctgcagcgt accatcttca
     9061 ccgccgcgac cgtgtagcgc gataaacact ttctgaaaac ccatcgactt cagttgcgtc
     9121 acgtcgactt ctttcgggtc gacaggatac gcgtcaatac cgccttcacg cagcccggct
     9181 aacaccgctg cgccagaatt cagagaaact tcccgctcag cggaggtccc gcccaacagg
     9241 accgcgattt tatcagtcat gttgttcttc ctccggagtt tgcggcttca gtttgatttc
     9301 agctaaagaa cgagcaattt ttccgatatt accagccccc tgaacgagaa tcaggtcgtt
     9361 accggttaat accggtgcca gcatctcggc tacctgcgcc gggtccggca ccagaatggg
     9421 atcaattttc ccacgtccac gaattgtgcg acacagcgaa cggctgtccg ctcccggaat
     9481 tggcgcttca ccagccggat acacttccag catcaacagg gtatcaacct gcgtcagcac
     9541 attggcgaaa tcatcataca ggtcgcgcgt acgggtaaaa cggtgcggct gaaacagcat
     9601 taccaggttt ttatccggcc agcctgcacg cgccgcttta atagtggcgt ccacttccgt
     9661 cgggtggtgg ccgtagtcat cgaccagcat tgccgtaccg cttttaccat tcactggctc
     9721 cagcgggaat tcaccgagga aatcgaaacg gcgccccgtc ccctggaagc tctccagcgc
     9781 acgcaaaata gcctcgtcgt caattccctc ttccgtagca accgcaaccg cagctgcggc
     9841 gttcagcgcg ttatgacgac ctggcgcatt cagggtgacg cgcatcggct ctttgtcctg
     9901 gcgcagcagc gtaaagtgcc cctgcgggcc aatctgctga taatcttcta cacgcacgtc
     9961 ggcatcttcg ctgaagccgt aagtcgtggt ctgacgcccc acacgcggta acaattcgcg
    10021 gatcaccgga tcatcaacac acatcaccgc acgaccgtaa aacggcaggt tgtgcagaaa
    10081 attaataaaa gtctgtttta aattctcaaa gtcgccctgg taggtatcca tgtggtcggc
    10141 ttcgatattg gtgacaatcg ccaccatcgg ttgcagatgc aggaacgatg catcactctc
    10201 atctgcttcg gcaatcaggt accgaccatg ccccaaacgc gcatgaaccc ccgccgcttt
    10261 taccagcccg ccgttaacga aggttgggtc gagccccgct tctgcgtaga tgctggaaac
    10321 catcgcggtg gttgtcgttt tgccgtgtgt tccggcaatg gcgatgccat gacgaaaacg
    10381 cattaactca gccagcattt cagcacgacg gatcaccgga atacgcgctt catgagctgc
    10441 aacaatttcc gggttatcgg cagaaatcgc gctggaaaca acgaccacgc tggcatcacg
    10501 tacgttttcc gggcgatggt tgaaataaat cgtcgctccc agattcatta actgctgcgt
    10561 gaccggattt ggcgctaaat cggaaccact gatctgataa ccttcattgg ccagaacttc
    10621 ggcaataccg cccataccgg caccaccgat gccgacaaaa tgtatgtgcc gaacgcgacg
    10681 catttcgggc acgatggaac gcagttttgc caattgttgt gtattcattc tttacgccat
    10741 taacttctta aattcatacg atgcaaaagg catcgctaca attacgcccg ggcagcccgg
    10801 ctcacttcat ttgccactcg ctcggtggca tccggaatgg atgcagcgcg ggcgcgttct
    10861 gccatggtta ataaggtttc tcgcgaccac ccggccaggg tgttggcgac agcatccacg
    10921 ctaagctgtg actgctcgat aattttggct gcgcccgctt tttccagcgg tagcgcattc
    10981 cagtattgct gacggtcttt atgttgaaac ggcacaaaca acgcaggtag tcctgccgcg
    11041 gcgatttcac tcaccgttaa cgcaccggag cggcaaacga cgacatccgc ccacgcatac
    11101 gccgccgcca tatcatcaat aaattccgtc actttatgct gcggttgccc cgcttcggca
    11161 tacgcctgtt caacggattg ttgcgaacct ttgccgctct gatgccagat agtgactgaa
    11221 tcacccagtt tcgcagcaac ctgcggcatt gtctggttaa gaatgcgtgc gccctgggaa
    11281 ccacccacta ccaacacacg aaccggacct tcacgtccag ccaaacgttg ctgcggcaac
    11341 ggcagcgcca gcacatcggt acgcaccggg ttacccacaa cttccgcatt aggaaaagca
    11401 cctggaaacg cctgcatcac tttggtagca atcttcgcca gccatttatt ggttaagccc
    11461 gcaataccgt tttgttcatg aagtacaacc ggaatgccta acgaccacgc ggccagacca
    11521 cctggacctg atacatagcc gcccataccg agcaccacgt caggtttgta cgctttcata
    11581 atcgcccgcg cctgacgcca ggcgttgaag atacgcagcg gcgcagctat cagtgctttt
    11641 ataccttttc cacgcagacc agagatacga atgaaatcaa tttcgatgcc atgttttggc
    11701 actaagtccg cttccatacg gtcggcagtc cccagccagc gaacttgcca accctgagcc
    11761 attagatggt gcgcaaccgc cagccccggg aatacatgtc caccggttcc gcctgccatc
    11821 accattaatc gcttcccttg accactcatc gtgaacctcg tacaaacgcc tgcgctttct
    11881 ccagacgcgt ttcataatca atacgcaaca gcatcatgat ggctgtcgac ataatcagta
    11941 agctcgaacc accgtaactg atcagcggca atgtcagacc tttggtcggt aacatccccg
    12001 ccgccgcgcc tacgttaacc agcgcctgga aactaaacca gataccaata gaacaggcga
    12061 gaaaaccgga aaaacggtgg tcaatttcta atgctttgcg gccaatcgac atcgcgcgaa
    12121 aagcgacgaa gaataccatt aaaagtgcca gcaccacacc gacatacccc agttcttcgc
    12181 cgataatggc gaaaataaag tcagtgtgcg cttccggcag atactccagt ttttgtaccg
    12241 agttacctaa accttgtccc cacagttcac cgcgaccaaa cgccatcaac gattgcgtta
    12301 actgatagcc gctgccaaag ggatcttccc acgggttcca gaatgcggta acacggcgga
    12361 tacggtacgg ttcggcgagg atcagcaaca caaccgctga aatgcccata ccgataatgg
    12421 caatgaactg ccacaatttc gctcccgcca ggaacaacat cgccagcgta gtcacaaaca
    12481 acaccaccac cgtaccaagg tctggctgtg ccagcagtaa cactgccaac accagaatta
    12541 cgcccatcgg tttcaggaag ccgcgcaggt tattacgtac ttcgtcgcct ttacgcacca
    12601 gatagttggc gatatagcaa aacagcgaca gttttgtcag ctccgcaggc tgaatacgca
    12661 gcaaaccgag atcgatccaa cgcgatgccc ctttaaccga gctacccact accagaacga
    12721 tcatcagcag gatgatagat ccgagcagca tcgtggcact gtagcgttgc cagaactcca
    12781 tcggtagacg acgcagcgta atgatcgcca gaatgaatgc caggatcaga tagacgccat
    12841 cacgcttcgc gaagaagaac ggatcgttgg ttaagcgttg ccctatgggc attgacgccg
    12901 aggtcaccat gataaagcca atcgccgcga ggccgaaggt cagccacagt aaggtgcgat
    12961 cgtacatgat caggctgttg gtatcttttt cccgcgagcc catcacccag ccctttagcg
    13021 ccgtggagat ccagaccaga atactgaatc ctggcaggcg cggcattttc aggcgaggga
    13081 gagataaacg catcaaccta actccttcgc cagacgggca aactcattgc ctcgttgttc
    13141 aaagttcttg aattgatcaa ggctggcaca ggccggagag agcagaacca tatcgcccgg
    13201 ctgaacacgc gtagcaagca agcgcatcgc ctgttccata gtttcggttt gttctgccac
    13261 ttccgggcgt agcgccgcca gctgcgcgcc gtcacgaccg aaacaataca gacgtacgtt
    13321 atcgccattc aggtaacgcg ccagtgggct aaagtccgcc gatttaccat cgccccccag
    13381 caacaaatgc agcgtgccgt ctacatgcag gccattcagc gccgcttccg tactgccgac
    13441 gttggtcgct tttgaatcgt taacccagca cacaccgtta tgctccagca caacttcaaa
    13501 gcgatgcggc agaccagtga atgtggttaa tgctttcagg ctgctggcac gcggtaaccc
    13561 tgcggcatct gccagcgcca gcgccgccag cgcattggtg tagttatgct gcccggaaag
    13621 tttcatttct ttcacgttca gcactttctc gcctttcacc cgcagccagg tttcgccctg
    13681 ctgatgattc aggtgatagt cgcccatgtt gacgccaaag ctgacgcagc gttcatccgc
    13741 accgcgaatc ggcattgtta aggcatcatc agcattaacc acgcaaactt ttgcgttttc
    13801 gtaaatgcgc agttttgctg cacgatactg ttgtaaacca aacggatagc gatccatatg
    13861 atcttcagtc acgttcagaa tggtcgctgc taccgcctgt aagctggagg tggtttccag
    13921 ctggaagctc gacagttcca gcacgtacag ttcgcactca tcatccaaca gcatcaacgc
    13981 aggcaggcca atattgccac ccacaccaac gttaaccccc gccgctttcg ccatttcacc
    14041 cactagcgtg gtgaccgtgc ttttgccgtt agaaccggta atcgccacaa tcggtgcttg
    14101 tgcttcacga cagaacagct cgatatcgcc aacgatttcg attccggcat cagcggcagc
    14161 gcttaaggat ggatgcgcca gtgcaatacc gggactggcg acaatcagat ctgccgccat
    14221 cagccattca tcattcagac cgcccgtgtg gcgttctacg gcttcgggta atttatccag
    14281 gccaggcggt gtcatacgcg tatccataac gcgcggcgtc acaccgcgag cgaggaaaaa
    14341 gtccacgcaa gaaagcccgg tgaggcccag gccgataatg acgacatttt taccctggta
    14401 atcagccatg attaacgtac cttcagcgtt gccagaccaa tcaggaccag catcagcgaa
    14461 ataatccaga aacgcacaat gacgcgcggt tccggccagc ctttcagttc atagtggtga
    14521 tgaatcggtg ccatgcggaa aatacgttgt ccgcgcagtt taaaggagcc gacctgcagg
    14581 atgacggaaa gcgtttctac cacgaacacg ccccccataa tcaccagcag gaattcctga
    14641 cgcagcagta cggcgataat acctaacgca ccacctaacg ccagcgaacc tacatcgccc
    14701 ataaagacct gcgccggata ggtgttaaac cacaggaagc ccagccctgc cccgactatc
    14761 gcggtacaga caatgaccag ttccccggcg tgtcgtagat acggtatgtg taaatagctg
    14821 gcaaagttca tgttgccggt cgcccacgcc accagcgcaa aaccaccggc gacaaatacg
    14881 gtcggcataa ttgccagacc gtcgagacca tcggtcaggt ttaccgcgtt gccagtaccc
    14941 acaatgacaa agtaagccag cagaatgtag aacagcccca gttgcggcat cacatcttta
    15001 aagaacggaa ccaccagctg cgttgcgggc gtgtctttgc cggcaaggta cagcgcgaag
    15061 gcaacaccca gtgcaatgac cgacatccag aaatacttcc aacgggcaat caggcccttg
    15121 gtgtctttac gcaccacttt gcgataatca tcaacaaagc caataacacc gtaacctacc
    15181 agcaccacca gtacgcacca gacgtacggg ttggaggggt aagcccacag cagtacggag
    15241 atcacaatcg ccgtcaggat cattatcccg cccatggtcg gcgtaccgcg cttactaaag
    15301 tgcgattccg ggccatcgtt acgaacgacc tgaccaaagg agagtttttg caaatgggca
    15361 atcatacgcg ggcccatcca caatgagatg aacagcgcgg tcagcaggct gacgatggcg
    15421 cgaaacgtca gataggaaaa gacgttaaag ccggaataat atttgaccaa atgttcggcc
    15481 agccaaacta acatgtccca ttctcctgta aagcgcgtac tacctcttcc atggcagcac
    15541 tacgtgaacc cttaactaaa atcgtaatta cctgttgctc agcaatcagt gatttaagac
    15601 gcgcgataag cgcagtttta tcggaaaaat gttcgccaac gccgctggca gtgctgatag
    15661 catggctttg tttacccaca cttaacacgc agtcaatacc agcagctttt gccgcttcgc
    15721 ccacctgtac atggcaggct tcgctttcag cgcccagttc cgccatatcg cccaccacca
    15781 gcacgcggta gcccggcatt tcagccagaa cctgaacagc ggcggtcatt gaaccgacat
    15841 tggcgttgta ggagtcgtcg agcagcaact ggttttctgc cagttttatg aggaacagac
    15901 ggcctggaac agctttcaga tttgccagcc ccgctttgat agcatcaagc gttgcgccca
    15961 cggacatgga gagcgcagcg gctgccagcg cattcgcaat attgtgacgc cccggcaacg
    16021 gcagcagaac atcgacgcta cctgttgggg tttgcagggt aaattccgta ccgtgcgagg
    16081 ttacatggat attggtggcg gtgaaatcgc tgttggcggc attaggtgag aaacgccaca
    16141 ctttgcgtga gccaattacg ctctgccagt tcaaccagtc gttgttgtcg gcgttcataa
    16201 tggcgatacc gttttccggc aggccgctaa agatttcacc tttcgctttc gcgacacccg
    16261 caagcgagcc aaaaccttcc agatgcgccg ctgccaggtt gttgaccagc gccgcttccg
    16321 ggcgagtcag actcacagtc caggcaattt caccctgatg gttcgcgcca agttcaataa
    16381 ctgcgtaatc gtattccggc gttaagcgca acagcgtcat cggtacaccg atgtcgttat
    16441 tgagattgcc tgccgtataa agtgtgttgc cgcactggct taaaattgcc gccgtcatct
    16501 ctttaacgga ggttttgccg gaggaacctg tcagagcaac cacgcgcgcc ggaacttgct
    16561 ggcgaaccca tgcagccagt tcaccaaacg ccagacgcgt atccttgacg attaactgcg
    16621 gcagatcaat atccagcgga cggctaacca gtagtgcgcc tgcgccgcca gctttcgcct
    16681 ggccggcaaa atcatgagca tcgaaacgtt cgcctttcag ggcaacaaac aggcagcccg
    16741 gcgtcagttt tcgcgtgtca gtggttacag catcaagggt aatatctgca ccttgcagtt
    16801 caccgttgag aatatcggta agttggctaa gggttacgct aatcatgcaa tcacccccag
    16861 cagacgcgcc accgtgacgc gatcggagta gtccagacgc tgattgccaa caatctggta
    16921 atcctcatgg cctttgcccg cgaccagtac cacatcattc tctttagcct gcataacggc
    16981 gcaagtcacc gcttcagcac ggccttccat cactttggca tatccggcat ctaacattcc
    17041 cgccagaata tcgttgatga tggcacgagg ctcttcggta cgcgggttat cgtccgtcac
    17101 caccgccacg tcagcaaact cttcagcaat tgcgcccatc agtggacgtt tgcctttatc
    17161 acgatcgcca ccgcagccaa agacgcacca cagcttgccc gcacagtgca ggcgcgccgc
    17221 ctgtaaggct ttttccagtg catccggcgt atgcgcgtaa tccaccacca ccgtcggttt
    17281 gcctggtgca gtgaacactt ccatacgtcc gcaaaccggt tgcagacgcg cggcggtttt
    17341 cagcaaatca gccagcggat agccgagtgc cagcagtgtc gccagcgcca gcagcaggtt
    17401 gctgacgttg aatgcgccca tcaggcgact ttcaatttcc ccatcgcccc aactcgagct
    17461 aaagcgaatg gtcgcaccgc tgtcgtgata gttcacgtcg gtcgctttca accagcgtcc
    17521 gtgacagttc ggattgatat gatcttccat tgataccgca accgcgtccg gcagttttgc
    17581 cagccagcgg cggcccactt catcgtcggc gttaataatc gcctgaccgc aatgatgtgc
    17641 agaataaagc agccatttcg cggcttcgta gtgttccata tcaccatgat aatcaaggtg
    17701 atcgcggctt aagttggtaa agaccgacgc cgtaaatttc aatgccgcca cacggtgttg
    17761 taccagcccg tgggatgaaa cttccattgc gcaaaaagtc gcgccctgat ccaccagccc
    17821 cgccagctca tgttgaacat cgactgccga accggttgta ttttctgtcg ggatcacttt
    17881 ccccagcagg ccgttaccaa cggtgcccat taccgcgctg gtttcgccaa gcagttggct
    17941 ccactgcgcc aacagctggg tagtcgtggt tttgccgttg gtgcccgtta cgcccacgag
    18001 acgtaaattg tcagagggtt catggtaaaa gcggcccgcc agtgcagata aacgctcgtt
    18061 gagctggctg agatagatga ccggtacgcc gtgcatttca cggatttcac cgtcggtcgc
    18121 ctcatctttc gcctctgcaa taatggcagc cacaccttgc gctatcgcct gcgggatata
    18181 tcgacgcccg tccgcctgat gacctactac agcaacaaag agatcgcccg ccgcagccac
    18241 acggctgtcg agtgtcatct ctcgcagtgc tcgcgaaggt gcgtctggca cccacggagc
    18301 aagaaggtcg cgcaaattac gatctgccac ctgtaccctc gccttgatta atcacaaatt
    18361 catttttatc gcccgttgtc agcgcatccg gctcgatgtt catggtacgc aatacgccgc
    18421 ccatgatggc accaaagacc ggcgcggaaa cggcgccgcc gtagtattta cccgcctgcg
    18481 gatcgttgat aacaacaacc agcgcgaagc gcggctgact cgcaggcgca acgcctgcgg
    18541 tataagcaat atatttattg atgtagcgac cgtccggccc gacctttttc gcggtaccgg
    18601 ttttaatggc gatacgatag cctttaatcg ccgccttcac gccgccgccg cccggtagcg
    18661 ccacgctttc catcatatgc accacggtgc gaacaatgga ttccgggaag acacgttcac
    18721 cgggaaccgg ggggtcaact ttggtaatcg acagtgggcg ataaatgccg tagctgccga
    18781 tagttgcgta gactcgcgct aactgtaacg gtgttaccat tagcccgtag ccgaaagaga
    18841 aggtggccct ctctatgtca gaccaccgtt gtttttgagg atataagcca ctgcgttctc
    18901 cgaccaaccc caaattggtc gcttttccca gtccaaaacg tgagtaagta tctactaacg
    18961 ctgaggacgg catcgctaac gccagtttgg aaacaccgac gttactcgac ttctgtaata
    19021 ccccggtcag ggttaattcg ctgtagcgcg ccacgtcttt gatttcgtgg ccgttaattc
    19081 gataaggaac ggtattgagt accgagtttt cccgcaccac gccacgttgc aacgcggtca
    19141 tcaccaccat cggtttaacc gttgagcccg gttcaaacac gtcggtgatg gtacggttac
    19201 gcatcgcttc tttcggcgtg ccgctcagat tatttgggtt gtatgacggg ctgttagcca
    19261 tcgccagcac ttcaccggtg ttgacatcca ccagcacggc gctaccagat tcagccttgt
    19321 taaaggccac cgcgttgttc agttcgcgat aaaccagcgc ctgcaggcgt tcatcaatac
    19381 tcagcgtcag gttgtgcgct gcctggctgt cagtagaaga aatatcttca attacgcgac
    19441 catagcggtc tttacgcaca atgcgctcac ccggctgccc ggtaagccat ttatcgaaac
    19501 tcttctcgac gccctcaatc ccttggctat cgacgttagt aaagccgatg aggtgagcag
    19561 tcacttcgcc ggatggataa taacggcgag attcttcacg cagatgaatc cccggcagtt
    19621 tcagtttttt gatgtagtcc gccatgtcag ggtttacctg acgcgccaga taaataaagc
    19681 gccctttcgg gttggcgtta atgcgggctg agagctgatc cagcggaata ttgagcgcgt
    19741 tagccagcgc cttccagcgg tcaccgacgc tgataccgcc agcgtcatgc acttctttcg
    19801 ggtcagccca aatcgctttt actggcacac tcaccgctaa cgggcgacca gaacggtcag
    19861 taatcatgcc gcgggaggtg gaaacttgct gaacgcgaag agaacgcatg tcgccctctt
    19921 tcaccagcat atccggggag ataacttgta accacgctac gcgtccgagc agaaaagcca
    19981 gcgccaggag aatacagccg cataacaacg caaaacgcca actgataaag ttggcatgtt
    20041 cttcctgacg ttttggtttc tgcgttttcg ccgctgcttt catgcgtcgc gtttatcctt
    20101 atttttgcac tacgatattt tcttgtgacg gatcaacatg ctgcatttgc agcttttccg
    20161 tggctatcct ttccacccgg ctatggtcgc cgagcgcatt ctcttcaagg atcaggttgc
    20221 gccattcaat gtctaaagca tctcgctcca gcaccagttg ttcgcgctga gcggtcagta
    20281 aacgggtatg gtgcgccgtg gttaccacgg tcaccgccgt caaaataatg caaatgaaca
    20341 ggcagagtgg cagcttccca aatcgcaaaa gatcgtcacc gataacgcca ggcaaggcat
    20401 ggcgctcgtg gcttcccatc gatcctttaa ctttgcttag agcttctgtc actctgctga
    20461 tcatgcgttc gtcctctctg caatacgcag aactgaacta cgggcacgag ggttctcagc
    20521 cacctcttct tcgcccggca tcaacttacc tagtgctcgc aactgacggc cccccagttt
    20581 tttgagctgc tcttctgtca tcggtaaccc tgccggaact tgcggaccgc ggctgttttc
    20641 acgcataaaa cgtttcacaa tgcggtcttc aagtgagtgg aagctgatga tcgaaagccg
    20701 cccccccggg gccagcacat tgagcgagct ttttagcgcc tgctctatct cctccagttc
    20761 actgtttacc caaatgcgca ccgcctggaa ggtacgggtc gcgggatgtt taaacttatc
    20821 tttcaccggc gttgcggcag ccacgacttc cgccagttct ttggtgcggg tcatcggctg
    20881 ttcgcggtta cgctcgacaa tagcgcgggc aatgcgtttg gcaaaacgct cttcaccata
    20941 ggttttcaat acccaggcga tatcggcttc ttctgcggtt tgtagccatt cagcggctga
    21001 ctgcccacgg gttgggtcca tacgcatgtc cagcggacca tcgcgcataa aggaaaagcc
    21061 gcgttcagca tcatcaagtt gcggtgaaga gacgccaaga tcgaggagaa tgccgtcgat
    21121 cttgccgata agatcgcgct cggcaacgta ttcgcccagc gcggagaaag gtccgtggat
    21181 gatggagaag cgcggatcat caatagtctt cgcaacggcg atagcctgcg ggtcgcgatc
    21241 gatcgccagc aaacgcccct cttcgccaag ctgcgagagg atcagacgtg agtggccacc
    21301 gcgaccaaaa gtcccatcaa tgtagatgcc atcaggacgg atattgaggc cattaacggc
    21361 ttcatccagc agcaccgtag tatgtttata gttttccatc attttataga gacaagtcct
    21421 gcagtcgctc cgataagtct ccggtagcca actgctctgc gtcgatatct tccttgacct
    21481 gttgatgcca ggttgtttca tcccacagct caaacttgtt gaactgtcca accagcatca
    21541 cttcttttgt cagcccggca tgttgccgca gtactggcgc gattaacaat cgacctgcgc
    21601 catccatctg acattcgctg gcatgaccta acagtaggcg ctgcacacgg cgctcaaccg
    21661 ggttcgtgct cgacagacgc gataattttt gctcgataat ttcccattca ggcagggggt
    21721 aaagcagcag gcacgggtga tgaatgtcaa tggtgcaaac catttgaccg gcagcgttct
    21781 caagcagctg ttcccgataa cgggtaggca ctgataagcg ccctttgctg tcgagattga
    21841 ctaacgttgc tccccggaac atgccagcct caccccttat tcccactttg ccccacaatt
    21901 tcccactgaa aggagtttac ggagcggagg aaaagcttgt caagccagtc acaaggcata
    21961 ccgaggtaaa aaacccgatg tttacggcta atatcagcgt tgtttatttt acgtccaacg
    22021 tgcgaagcaa attaacgtta tgaatagctg taagaaaaaa tccctcatcg tcatcaccgt
    22081 tttaaaatga ctcactattt tccacagcaa atataaagtg tcagtttgcg acgcgagcgg
    22141 cattttagga catatcttcc caggttaaca gtccctgttg cgtctgtgtg gcgcttgatg
    22201 ccagcgggtt tgcgcaaaag caaagaaaag tgtttgttta atcggcaatt catctctgca
    22261 tgtaacaaaa taatgcaaaa acgcgctatc gatatcactt aattattgtg aatttcagct
    22321 ggtgaggtaa ttaacgggca ggtttaaaag catttatcaa taatagccag gaatagtctt
    22381 atttacttta aggatattga tgtccattcc cgtactcaac gcgccagaga gaaattcacc
    22441 tggcgcgtat ttttgttcgc ggcttagcta cggctgagca cgccgcggcg atagagatta
    22501 cgtttaatgc gcgttaaacc aggttttggc ttacgcggtt catcgaggct cgccaggaca
    22561 atctccagca cacgttctgc gacatcgcgg tgacgttgag ccactgccag caccgggcac
    22621 tgtaagaagt cgagcagttc gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt
    22681 ttgccgtcgc gacgtagcgt gacatccatc accccttgca acagcgcaaa cgacgttgtg
    22741 aacagcgcct gcggcatcgg atgcgtttcc agccattttt cgaataactg ggcagctgcc
    22801 tcccgctcat agctgttggc ataaaggaaa tgcacttcgc gcggatcatc tttccaggca
    22861 gtacggaagc cttgttcacg caggaagctg acagaaagct ccggcagcgc gccaagataa
    22921 agcaccgtct cggcgggaaa cttacgtaac tcttccgcca gcatttcggc atcatcctga
    22981 tcggcaccaa ccacgctggt gaagtgttca cgatcgaggg cgcggtccag cgcgacaatc
    23041 gggaacgggt cgttagccca gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa
    23101 acaataatgg catcaacctg acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtcg
    23161 ggctgatctt ccgagcaggc aatcagcagt tgataacccc gttgccgcgc ctggcgttca
    23221 agatagttag cgatgcgggt atagctggtg ttctccagat cggggatcac aagaccaata
    23281 gaacgtgtgc gtccagcacg aagcccagct gccacggcgt tcgggtggta attgtgctca
    23341 cgcaccacag ccatgacttt ttcaacggtt ttgtcgctca cacggtattg cttcgctttg
    23401 ccgttaataa catagcttgc agtggtccgc gacactcccg ccagccgagc gatttcatcc
    23461 agtttcacaa ttgccccttg cgtaaaatgt aaaaaccata gccattgtac tggtatggat
    23521 taaattcatt aacatctaag cgcagaaaat taactgcggc aactgctttt attccaggtt
    23581 cccactgatt tcgcaaaaaa agcccaacgt gacatgttgg gctgcaaatt gcgcattgag
    23641 atcattctca acgcattatt ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa
    23701 cttccacaat tttcgccaca tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg
    23761 tgcctgctaa ttgaacggta taaagcgagg gtgtgacatc gataatttgc ccacggaata
    23821 tttccgtatt acgtttcact tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca
    23881 tgatttcccg ctcaacgtgc gcgccctgcc ccaactcgct cacgcgcaag acatcgacca
    23941 gcttgtgtaa ttgcttttcg atttgctcaa gcactttttc atcgcccacg gtctggatgg
    24001 tcatacgcga taatgtcgga tcgtcggttg gcgcaacggt caggctttca atgttgtagc
    24061 cacgctggga aaaaaggcca atcacgcggg ataacgcgcc tgattcattt tcgagtaaga
    24121 ctgataatat ccggcgcata atcaggttct ctccgttttg cttaaccaca tttcatccat
    24181 tccgcccccg cgaatctgca tcgggtagac gtgctcactg ccatcgacgg taacatcaac
    24241 aaacaccagg cgattattgc gcacctgttc cagcgcctcg ctaagtttgc tttccagttc
    24301 ttgcggatga gagatctgga tcccgacgtg accataggct tccgccagac ggacgaagtc
    24361 tggtaaggac tgcatataag attgtgaatg acggccggaa tagatcatgt cctgccactg
    24421 cttcaccatc cccagatagc ggttattgag attcaccacc agtacgggca actcatattg
    24481 caacgcggta gacagttcct ggatgttcat ctgaatactg ccgtcaccag tgacgcaaac
    24541 cacggtttct tctggcaacg ccattttgac gcccagtgcc gcaggtaaac caaaacccat
    24601 cgtgccgagg ccgccagagt tgatccagcg acgcggtttg tcgaatggat aataaagcgc
    24661 agcaaacatc tggtgctgcc cgacatcgga cgtcacgtaa gcgtccccat gagtcaaccg
    24721 ccaaagagtc tcgatcaccg cctgcggttt aatcttttca ctgtgagtgt catatttcag
    24781 gcactgacga gcgcgccact gttcaatctg ctgccaccag tcgcggatct catccagcgg
    24841 ttgatgggcg gattcttgcg acaggagttc aagcatttgt tctaggacct ggcgagcatc
    24901 ccccacaatc gggatatcag cagtcacggt tttagaaatt gaggttggat caatatcgat
    24961 atgcagaaca gtggcatttg ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa
    25021 acgtaccccg acggcgaaaa tcacatccgc gttatgcatc gtcatattgg cttcgtaggt
    25081 accgtgcatt cccaacatgc ccagtgcctg gcgatgcgtt gccggaaacg cccccagccc
    25141 catcaaagag gaaacaacgg gcagattcaa cacctccacc gcttctttca actgcagatg
    25201 gcagcccgcc gtgattgccc caccgcctac gtagacaacc ggtttttttg ccgccaccag
    25261 cgtttgcaga gcgcgcttaa tttgcccttt atgcccggag gtcgtgggat tgtaagaacg
    25321 catactgacc gactccggcc agacataggg taatttgttc gccggattta gaatatcttt
    25381 cggtaaatca acgaccacgg gtccaggacg accacttgcc gctagccaga aagccttttt
    25441 cagcacctgc ggaatgtctt ccgtttgctt aaccagaaaa ctgtgtttaa ccaccgggcg
    25501 agaaatcccc accatgtcgc actcctgaaa ggcatcataa cctatcaacg aggtcgctac
    25561 ctgcccggaa aggacaacta atggaatgga atccatataa gcggtggcaa tgccagtaat
    25621 cgcattggtc gcccctggac ccgacgttac cagcacgacg ccgacttccc cggtcgcgcg
    25681 cgccagacca tcggccatat gcaccgccgc ctgctcatgg cgaactaaaa catgatcgat
    25741 accacccacg gtatgcaatg catcgtaaat atcaaggacc gcgcctccag gataaccgaa
    25801 tacttgttta acgccctgat cgataagcga tcggacgacc atctcggctc cagacaacat
    25861 ctccatggcc tgcctcactg tttgacggaa aaaatgtgta aaacacatta accgtccgat
    25921 cttgcttaag caattggcag acagagacat aaacacgggg taataagaat aaacaggagg
    25981 aaaggtgaaa agaaacgata aaatcctcca ttacagagga tgaaattgat aataaatcgg
    26041 ctgaatccta caacttacag caatttctct atttttttaa ttaatcctaa aatagagagc
    26101 acaaacattc agaataaaat tctacattca ataaaaaatg cagaataaac cagacattga
    26161 tgtctgacct attctgcatt cagttagcgt ttgcaaattg agactaattg ctcttccatc
    26221 cactgatggc ctttatcgcg cccggcagct tcatgccagg agagataaca ggttctgctg
    26281 ttttgtttta acggcagcgg taatacctgt aactctaagg attcagcgaa ctcttcagcc
    26341 aaccaacgcg gcgcaatagc gaccagatgc gtttgcgaca ccacgctaag tacgctcatc
    26401 attgccatgc cctgatacgc gatactggct tgcttatcta ccgtgtcata ccaggtttga
    26461 ctaaatgatg cgaaacgatc gagcgaaacc gcagcgtgtt gttcgttata aacatcatgc
    26521 ttcagtaacg ggcctttaat tgttggatga tttttgctgg ctaccaacac catttcatct
    26581 ttaaataatg gcacactggt aaattcaggg cgatggaagt cttcataact aatcacaaac
    26641 tccgtttcct gataacgcag ctgatgttca gtgttctgat ttaatgaaga cttgaacata
    26701 acatgtatat ttggcgcaat ctgctcaatg tgattataaa tctgcgaggt cagaatgctg
    26761 tctaacgggc tgcaaacaca aagatgaaat acacgttcac tgctcgcggg ttcaaaacct
    26821 gaaccaggca attcattttg tactagttgc aatgcctgac gaactgagcc aaaaagttga
    26881 aatgcgcgag cagtcggttg aataccacgg ccataacgaa caaaaagctc gtcattaaac
    26941 atcaccttca ggcgtgcaac agcgttactg accgcaggtt gcgacattcc cagagcatga
    27001 gcggcacggg tgatgttttg ctcctgcatc acggcatcga aaacggttaa taagttgaga
    27061 tcgaccatgc gtagctgtgg cttgcttaac tccgccgtct ctggatgatc tgtttgtacc
    27121 tctggcatac ttaactccac tgtcacactt aactcccttt cccttattgg aatgcagaga
    27181 aataatcctc gagaatatga tttatcatgc atataaaata agaaaaagca aaatgagtaa
    27241 aattaggaaa acataaagat acaggttctg ataaaaacag aatccactg
//
LOCUS       DAZFJL010000061        26780 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFJL010000061 DAZFJL010000000
VERSION     DAZFJL010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.061"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(157..819)
                     /gene="fsa"
                     /locus_tag="ACHJ8C_002438"
     CDS             complement(157..819)
                     /gene="fsa"
                     /locus_tag="ACHJ8C_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO5552282.1"
                     /translation="MELYLDTSDVVAVKTLSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKSE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            950..1849
                     /gene="ybiY"
                     /locus_tag="ACHJ8C_002439"
     CDS             950..1849
                     /gene="ybiY"
                     /locus_tag="ACHJ8C_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO5552283.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMGLLQASHEAGIHTAVETC
                     LHVPWKYIAPSLSYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            1855..4287
                     /gene="ybiW"
                     /locus_tag="ACHJ8C_002440"
     CDS             1855..4287
                     /gene="ybiW"
                     /locus_tag="ACHJ8C_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO5552284.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQAKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            4433..5248
                     /gene="ybiV"
                     /locus_tag="ACHJ8C_002441"
     CDS             4433..5248
                     /gene="ybiV"
                     /locus_tag="ACHJ8C_002441"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO5552285.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLENKPPFNL"
     gene            complement(5448..7040)
                     /gene="ybiT"
                     /locus_tag="ACHJ8C_002442"
     CDS             complement(5448..7040)
                     /gene="ybiT"
                     /locus_tag="ACHJ8C_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO5552286.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            7259..8179
                     /gene="ldtB"
                     /locus_tag="ACHJ8C_002443"
     CDS             7259..8179
                     /gene="ldtB"
                     /locus_tag="ACHJ8C_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO5552287.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(8237..9355)
                     /gene="ybiR"
                     /locus_tag="ACHJ8C_002444"
     CDS             complement(8237..9355)
                     /gene="ybiR"
                     /locus_tag="ACHJ8C_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO5552288.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPVLQGVLSNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAG"
     gene            complement(9352..9819)
                     /gene="mntR"
                     /locus_tag="ACHJ8C_002445"
     CDS             complement(9352..9819)
                     /gene="mntR"
                     /locus_tag="ACHJ8C_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO5552289.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            10005..10133
                     /gene="mntS"
                     /locus_tag="ACHJ8C_002446"
     CDS             10005..10133
                     /gene="mntS"
                     /locus_tag="ACHJ8C_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO5552290.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            10405..11988
                     /gene="opgE"
                     /locus_tag="ACHJ8C_002447"
     CDS             10405..11988
                     /gene="opgE"
                     /locus_tag="ACHJ8C_002447"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO5552291.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGIFIFALGVIAIRRKKESEKARWNTFDSLCLVFSAATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(12038..12553)
                     /gene="ompX"
                     /locus_tag="ACHJ8C_002448"
     CDS             complement(12038..12553)
                     /gene="ompX"
                     /locus_tag="ACHJ8C_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO5552292.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(12606..12671)
                     /locus_tag="ACHJ8C_002449"
     CDS             complement(12606..12671)
                     /locus_tag="ACHJ8C_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5552293.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            12906..13793
                     /gene="rhtA"
                     /locus_tag="ACHJ8C_002450"
     CDS             12906..13793
                     /gene="rhtA"
                     /locus_tag="ACHJ8C_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO5552294.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKERKIKELDIN"
     gene            14093..14596
                     /gene="dps"
                     /locus_tag="ACHJ8C_002451"
                     /gene_synonym="pexB"
     CDS             14093..14596
                     /gene="dps"
                     /locus_tag="ACHJ8C_002451"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO5552295.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            15000..15746
                     /gene="glnH"
                     /locus_tag="ACHJ8C_002452"
     CDS             15000..15746
                     /gene="glnH"
                     /locus_tag="ACHJ8C_002452"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO5552296.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            15885..16544
                     /gene="glnP"
                     /locus_tag="ACHJ8C_002453"
     CDS             15885..16544
                     /gene="glnP"
                     /locus_tag="ACHJ8C_002453"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO5552297.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            16541..17263
                     /gene="glnQ"
                     /locus_tag="ACHJ8C_002454"
     CDS             16541..17263
                     /gene="glnQ"
                     /locus_tag="ACHJ8C_002454"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO5552298.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            17380..19605
                     /gene="ybiO"
                     /locus_tag="ACHJ8C_002455"
     CDS             17380..19605
                     /gene="ybiO"
                     /locus_tag="ACHJ8C_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO5552299.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVTELMENEDIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAPPAEPLPPGEPT
                     L"
     gene            complement(19602..20528)
                     /gene="rlmF"
                     /locus_tag="ACHJ8C_002456"
     CDS             complement(19602..20528)
                     /gene="rlmF"
                     /locus_tag="ACHJ8C_002456"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO5552300.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            20804..21064
                     /gene="mcbA"
                     /locus_tag="ACHJ8C_002457"
     CDS             20804..21064
                     /gene="mcbA"
                     /locus_tag="ACHJ8C_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO5552301.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTAIIYK"
     gene            21329..23611
                     /gene="fiu"
                     /locus_tag="ACHJ8C_002458"
     CDS             21329..23611
                     /gene="fiu"
                     /locus_tag="ACHJ8C_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO5552302.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGAIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQMIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            23827..24504
                     /gene="ybiX"
                     /locus_tag="ACHJ8C_002459"
     CDS             23827..24504
                     /gene="ybiX"
                     /locus_tag="ACHJ8C_002459"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO5552303.1"
                     /translation="MMYHIPGVLSPQDVARFREHLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPESYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSHYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            24578..24844
                     /gene="ybiI"
                     /locus_tag="ACHJ8C_002460"
     CDS             24578..24844
                     /gene="ybiI"
                     /locus_tag="ACHJ8C_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO5552304.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     LIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            25109..25369
                     /gene="ybiJ"
                     /locus_tag="ACHJ8C_002461"
     CDS             25109..25369
                     /gene="ybiJ"
                     /locus_tag="ACHJ8C_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO5552305.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(25472..25531)
                     /gene="ybiE"
                     /locus_tag="ACHJ8C_002462"
     CDS             complement(25472..25531)
                     /gene="ybiE"
                     /locus_tag="ACHJ8C_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO5552306.1"
                     /translation="MRLSANRQGNTCAERSVNA"
     gene            complement(25597..26682)
                     /gene="hcxB"
                     /locus_tag="ACHJ8C_002463"
     CDS             complement(25597..26682)
                     /gene="hcxB"
                     /locus_tag="ACHJ8C_002463"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO5552307.1"
                     /translation="MVSGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKVVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
BASE COUNT         6602 a         7090 c         6605 g         6483 t
ORIGIN      
        1 agcgtcgcat ccggcatttg ctccagacgc cagatgcggc gtgaacgcct tatctggcct
       61 acggttctga cgcagatttc caggcctgat agacgcggca ggcatcggtg cactccgcca
      121 cgctatcagc aatttatgag gatgcagaac gtccggttaa atcgacgttc tgccaaacgc
      181 cccttgccag tcctgctcaa acttcgctac ggcggcgtca acagccggat agctaatcat
      241 ctgttgtgcc acatccagcg gcagggtgat tgactcacac ccagccagta agcagtccag
      301 cgcctgacgc ggggttttga aactcgcggc taacactttc gcctgcggcg catgcatttt
      361 caataactgg tgtaagtcgg tcacagtctg aataccgcta ccgccctgag catcaatacg
      421 attaacgtaa ggcgcaacat attccgcacc tgccagcgcc gacagtagcc cttgtgctgc
      481 gccatatacc gcggttccca gcgtcggaat cccttccgat tttaacatct taatagctgc
      541 cagcccctca gcggtcaccg gaactttcac cacgatatcc gcaataatag aacgcaattt
      601 acgtgcgtca ttcaccatcc cttcagcagt ggtagccatt acctgggcaa atagacgccc
      661 ctgaccgccc atcgcctcgt gaagttgcgg aagcacaacc tccagcggtt ttttacccgc
      721 tgcgataatg cttgggttag tggtcacacc cgccagcgga aaaatacgtg acagcgtctt
      781 caccgcaaca acgtctgaag tatccagata cagttccata accatcctca aaatgtactt
      841 tttgtctgaa tgtagactat cacgggaaaa ctctctgaac agttgacgca catcaatcta
      901 actttcattc gaaagtattt taatgtttga acgaaacaga gaggcaacta tgattttcaa
      961 tattcaacgc tactcgaccc atgatggccc cggtatacgc acggtcgtat ttcttaaagg
     1021 ctgttcgctg ggctgccgct ggtgtcagaa cccggaaagc cgcgcccgca cgcaggatct
     1081 gctgtatgac ccacgactct gtctggaagg ctgtgagctg tgcgctaaga ccgcaccgga
     1141 agtgattgag cgcgcgttga atggtttgct tattcatcgg gaaaagttaa ccccggagca
     1201 ttttacggcg ctaaccgact gctgcccaac acaggcgtta accgtgtgtg gtgaagtgaa
     1261 aagcgttgag gagatcatgg caaccgtttt gcgcgataaa ccgttttacg atcgcagcgg
     1321 cggcggctta acactttctg gcggagaacc gtttatgcag ccggaaatgg cgatggggct
     1381 actgcaagcc agccacgaag ccggtattca tactgcggta gaaacctgtc tgcatgtgcc
     1441 gtggaaatat atcgcccctt ctctgtccta tatcgatctg tttcttgccg atttaaaaca
     1501 cgttgccgac gcgccgttta aacagtggac cgacggtaac gccgccaggg tgctggataa
     1561 cctgaaaaaa ctcgccgcag ccggcaaaaa aatcattatc cgcgtgccac tgattcaggg
     1621 ctttaatgcc gacgaaactt ctgtgaaagc cattaccgat tttgccgccg acgagctgca
     1681 cgttagcgaa attcattttc tgccctacca cacactgggc atcaacaaat atcacttact
     1741 taatctgccc tatgacgccc cggaaaaacc gcttgatgcg ccagaactgc tcgactttgc
     1801 ccagcagtat gcctgccaga aagggttaac cgcgacctta cgaggataac aaccatgacc
     1861 acactgaaac tggacacgct cagcgaccgc attaaagcgc acaaaaatgc gctggtgcat
     1921 attgtgaaac caccagtctg taccgagcgc gcgcagcact ataccgagat gtatcaacaa
     1981 catctcgaca agcccattcc ggtacgtcgc gcgctggcac tggcgcatca cctggcgaat
     2041 cgcaccatct ggatcaaaca tgatgaattg atcattggca accaggcaag cgaagttcgc
     2101 gccgcgccga tcttcccgga atataccgtc tcgtggatcg aaaaagagat tgatgatctg
     2161 gcagatcgtc ccggtgctgg ttttgcggtg agcgaagaga acaaacgcgt tctgcatgaa
     2221 gtgtgcccgt ggtggcgcgg tcagaccgta caggatcgct gctacggcat gtttaccgat
     2281 gagcaaaaag gtctgctggc gaccggaatc attaaagcgg agggcaatat gacctccggc
     2341 gatgcgcacc tggcggtcaa tttcccgcta ctgctggaaa aaggtcttga tggtctgcgt
     2401 gagaaagtgg cggaacgtcg ctcgcgcatc aatctgacgg ttctggaaga tctgcacggc
     2461 gagcaattcc tgaaagcgat tgatatcgtg ctggtggcag tcagtgaaca cattgaacgt
     2521 ttcgctgccc tggcgcgtga aatggccgca accgaaaccc gcgaaagccg tcgcgatgaa
     2581 ctgctgacga tagcagaaaa ctgcgatctt atcgcccacc agccgccgca gactttctgg
     2641 caggcgctgc aactgtgcta cttcatccag ttgattttgc agatcgaatc taacggtcac
     2701 tcggtatcgt ttggtcgtat ggaccagtat ctctacccat actatcgccg tgacgttgaa
     2761 ctgaaccaga cactggatcg cgaacacgcc atcgagatgc tgcatagctg ctggctgaaa
     2821 ctgctggaag tgaacaaaat ccgctccggc tcacactcaa aagcctctgc gggaagtccg
     2881 ctgtatcaga acgtcaccat tggcgggcaa aatctggttg atggtcaacc gatggacgcg
     2941 gtaaatccac tctcttatgc gatcctcgaa tcttgcggtc gcctgcgttc gactcagcct
     3001 aacctcagcg tgcgttacca tgcgggtatg agtaacgatt tcctcgacgc ctgtgttcag
     3061 gtgatccgct gcggcttcgg gatgccggcg ttcaacaacg acgaaatcgt gatcccggaa
     3121 tttattaaac tcggtattga accgcaggac gcttacgact acgcagcaat tggttgtatc
     3181 gaaaccgccg tcggtggcaa atggggctat cgctgtaccg gcatgagctt tatcaacttc
     3241 gcccgcgtga tgctggcggc gctggaaggc ggtcgtgatg ccaccagcgg caaagtgttc
     3301 ctgccacaag caaaagcgtt gtcagcgggt aacttcaaca acttcgatga agtaatggac
     3361 gcatgggata cgcaaatccg ttactacacg cgtaaatcaa tcgaaatcga atatgtcgtc
     3421 gacaccatgc tggaagagaa cgtacacgat attctctgct cggcgctggt cgatgactgt
     3481 attgagcgag cgaaaagtat caagcaaggc ggcgcgaaat atgactgggt ttctggcctg
     3541 caggtcggca ttgctaacct cggcaacagc ctggcggcag tgaagaaact ggtatttgaa
     3601 cagggcgcga ttggtcagca acagctggct gccgcactgg cggatgactt cgacggtctg
     3661 actcacgagc agctgcgtca gcggctgatt aacggtgcgc cgaagtacgg caacgacgat
     3721 gatactgtcg atacgctgct ggctcgcgct tatcagacct atatcgacga actaaaacag
     3781 taccataacc cgcgctatgg tcgtggtccg gttggcggca attattacgc gggtacgtca
     3841 tctatctccg ctaacgtacc ctttggcgcg cagacgatgg caacgccgga cggacgtaaa
     3901 gcgcataccc cgctggcaga aggcgctagt ccggcctccg gtactgatca tcttggccct
     3961 actgcggtca ttggctcagt gggtaaactg cctacggccg cgattctcgg cggcgtgctg
     4021 ctcaaccaga aactgaatcc agcaacgctg gagaacgaat ctgacaagca gaaactgatg
     4081 atactgctac gcaccttctt tgaagtgcat aaaggctggc atattcagta caacatcgtt
     4141 tcccgcgaaa cgctgctgga agcgaaaaaa catccggatc agtaccgcga tctggtagtg
     4201 cgtgtcgcgg gctattccgc cttcttcacc gcgctctctc cagacgctca ggacgatatc
     4261 atcgcccgta ctgaacatat gctgtaatct cctcagcccg gcggcgtcgt cgccgggcca
     4321 aatcattcac atcatcaatt tcagcctcgc tttcattcga aatataattt gtgctctgcg
     4381 tcacattgtt attacactgt tacggtttta ttttcgagcc aggagcacac ctatgagcgt
     4441 aaaagttatc gtcacagaca tggacggtac ttttcttaac gacgccaaaa cgtacaacca
     4501 accacgtttt atggcgcagt atcaggaact taaaaagcgc ggcattgagt tcgttgtcgc
     4561 cagcggtaat cagtattacc agcttatttc gttctttcct gagctaaagg atgagatctc
     4621 ttttgtcgcg gaaaacggcg cactggttta cgaacatggc aagcagttgt tccacggcga
     4681 actgacccga catgaatcgc ggattgttat tggcgagttg ctaaaagata agcaactcaa
     4741 ttttgtcgcc tgcggtctgc aaagtgcata tgtcagcgaa aacgcccccg aagcattcgt
     4801 cgcactgatg gcaaaacact accatcgcct gaaacctgta aaagattatc aggagattga
     4861 cgacgtactg ttcaagtttt cgctcaacct gccggatgaa caaatcccgt tagtgatcga
     4921 caaactgcac atagcgctgg atggcattat gaaacccgtc accagtggtt ttggctttat
     4981 cgacttgatt attcccggtc tacataaagc aaacggtatt tcgcggttac tgaaacgctg
     5041 ggatctgtca ccgcaaaatg tggtagcgat tggcgacagc ggtaacgatg cggagatgct
     5101 gaaaatggcg cgttattcct ttgcgatggg taatgctgcg gaaaacatta aacaaatcgc
     5161 ccgttacgct accgatgata ataatcatga aggcgcgctg aatgtgattc aggcggtgct
     5221 ggaaaataaa ccgccgttta atctttgatt atcgccaatt cagtttattg agaaagtgca
     5281 tgtcattata ccggatacgg cgtgaccgcc ttatttggca tacaaaatca tgaaaattca
     5341 atatattgcg atacactacc aggcctgata agcgtagcgc accaggcaat cttgcgtttg
     5401 tcatcagttt cagtagccct gaccatatca gggctactga taataactta ctcgatccct
     5461 ttactacgca ggtaatcttc gtaattaccg ctaaagtcga tcacgcgttc cggggtgatt
     5521 tccagaatac gggtcgccag ggagcttaca aactcacggt cgtgagaaac aaagatcagc
     5581 gtgccctgat acagttccag cgccatgttc agcgactcga tggattccat atccaggtgg
     5641 ttggtcggct cgtccatgat cagaatgttc ggcttctgca tcattaattt accaaacagc
     5701 atccgccctt tttctccacc ggaaagcact ttcgctggct ttttgatgtc gtcctggctg
     5761 aacagcaaac gaccgagaat gctgcgtacc gcctgctcat catcgccttc ctgcttccac
     5821 tgactcatcc attcgaacac ggtcagatca ttttcgaact catattcatg gtcctgggcg
     5881 tagtaaccaa tgcgcgcgtt ctcagaccat tttacggtgc cgctgtccgg ttgcagatcg
     5941 cccaccagcg ttttcagcag cgttgattta ccgacgccgt tggtacccag tacagccagt
     6001 ttttcaccca cttccagcag caggttgaga tttttaaaca gtggaccgtt atcaaaccct
     6061 ttggtcagac cttccacttc cagcgcgtta cggaacagtt tcttatcctg ctcaaaacgg
     6121 atgaacgggt tctgacggct ggaggctttc acctcttcca gtttgatttt atcaatctgg
     6181 cgcgcgcgcg aagttgcctg gcgagatttc gaggcgttgg cactaaagcg gctaacgaaa
     6241 gattgcagct cagcaatctg cgctttcttc ttggcgttat cagccagcag acgctcacgc
     6301 gcctgggtcg ccgccgtcat gtactcatcg tagttgcccg gataaacgcg cagctcgccg
     6361 taatccagat ccgccatgtg ggtacaaacc atgttaagga agtgacggtc gtgcgagatg
     6421 atgatcatgg tgctgtcacg ctcattcagc acctgttcca gccagcgaat ggtgtcgatg
     6481 tcgaggttgt tggtcggttc gtcgagcagg agaatgtccg gatcagcaaa cagcgcctgc
     6541 gccagcagca cacgcagctt ccagccagga gcaacttcac tcatcgggcc gtagtgctgt
     6601 tccactggaa ttcccacgcc aagcaacagt tcaccagcgc gagcttccgc agagtaaccg
     6661 tccatttcgc cgtatttcac ttccagatcg gccactttat agccgtcttc ttcactcatt
     6721 tccggcaaag catagatgcg gtcacgctcc tgcttcactt cccacaactc tttatgcccc
     6781 atgatcaccg tatccagcac agtgaactct tcaaaggcaa actgatcctg acgcagctta
     6841 ccaatgcgct cgttgggatc gagggaaacg ttacccaacg tcggctctaa gtcgccgccg
     6901 aggatcttca taaaggtgga tttaccacta ccgttcgcgc caatcaggcc gtaacggttg
     6961 ccgccgccga atttgacgga aatgttttca aacaacggct tactgccgaa ctgcatggtg
     7021 acgttactgg aaactaacac aggcgtatcc tgaaaagaga tatgacaaac cgcgtattat
     7081 gccataagtc tgaaagaaga tcctgctcct cgcttaaggt ctacactcaa gccaaaggca
     7141 aaaaatgtga tttcgtacac atctgatttc actgtgagct ggaatgaact tataatgcac
     7201 ttccaatact ctaatattct caacccaatg gcctgccagg cacaaaatct cgcttaacat
     7261 gaatatgaaa ttgaaaacat tattcgcagc ggccttcgct gttgtcggct tttgcagtac
     7321 cgcctctgcg gtaacttatc ctctgccaac cgacgggagt cgcctggttg gtcagaatca
     7381 ggtgatcacc attcctgaag gtaacactca gccgctggag tattttgccg ccgagtacca
     7441 gatgggactt tccaatatga tggaagcgaa cccgggtgtg gataccttcc tgccgaaagg
     7501 cggtactgta ctgaacattc cgcagcagct gatcctgccg gataccgttc atgaaggcat
     7561 cgtcattaac agtgcagaga tgcgtctgta ttactatccg aaagggacca acaccgttat
     7621 cgtgctgccg atcggcattg gtcagttagg caaagacacg cctatcaact ggaccaccaa
     7681 agttgagcgt aaaaaagcag gcccgacctg gacgccgacc gctaaaatgc acgcagagta
     7741 ccgtgctgcg ggcgaaccgc ttccggcggt cgttccggct ggtccggata acccgatggg
     7801 gctgtatgca ctctacatcg gtcgcctgta cgctatccat ggcaccaacg ccaacttcgg
     7861 tatcggcctg cgtgtaagtc atggttgtgt gcgtctgcgt aacgaagaca tcaaattcct
     7921 gttcgagaaa gtaccggtcg gtacccgcgt acagtttatt gatgagccgg taaaagcgac
     7981 caccgagcca gacggcagcc gttatattga agtccataac ccgctgtcta ccactgaagc
     8041 ccagtttgaa ggtcaggaaa ttgtgccaat taccctgacg aagagcgtgc agacagtgac
     8101 cggtcagcca gatgttgacc aggttgttct ggatgaagcg atcaaaaatc gttccgggat
     8161 gccggttcgt ctgaattaat cttcaaaacg taaagcaaag gcggactcat aatccgcctt
     8221 tttaatttga cagaccttat cccgccggga tcataaccaa caaaacatat cccaccagcg
     8281 ccgcccacaa cagcatcggt atcgaataaa gatgaaaacg ccaccagata cggcgatcgt
     8341 tcgccatccg taacgcaatc aaatttgcca gcgatccggg taataaacca aagcccccca
     8401 cgtttaccgc ccatgccagt aataaagacg gtggcacata attgagcagc aaaatggtac
     8461 tcggcacgtt actgatcacc tgcgataaac cgatcgccgt taaccataat ccgggttcgg
     8521 ataaatgact cacgttactt aacaccccct gcaaaactgg cagttgggtt agtaaatgga
     8581 cgtcgataaa catcgccata aacaccagca gcagcgtcca gtccacactg agcaccacac
     8641 gacgcgccag cagcgcaaag cccgccgcca caatcaccag tccccacagc tcttgtttta
     8701 actccagcgc cgtcagaaag acgatataca gccccagaca actccacacc agccgcggtt
     8761 tccactccgg cgtttgcacc cccgtatggt attgcagcgc ctttccaggg aaacaacacc
     8821 agcacaacag caggagtgtc agcatcattg cgccagccag cggtgccatt tgggcaataa
     8881 atccggcaaa cgaaagacca gaacgtcccc agataagaat attttgcggg ttgccaattg
     8941 gcgtcagtag cgaaccagcg ttgactgcca gcgcctcaaa aataatcagc cgattaaccg
     9001 ggatctcaca cagtcttttt agcgtgatag tcagcggaac aactataaac agcgcgacat
     9061 cgttggtcag aaaggtagaa agcagcgccg ccgccagcac cataaacatc gccagccgac
     9121 gctccgtagc aaagcggcgc accattttgc gccccagcac atcaaaataa ccgcttaact
     9181 ccacaccttt ggtcagcagc atcaggccgc ttaaggtgat gatggtgtgc cagtcgatag
     9241 cagcaggcca ggatttcggt gcaaaaggca cgaaaaagct taatccgata ccaacaagaa
     9301 ttaataactg aaaaaaacga tcaccttgca gcgtgcgtaa aaaaggcagg ctcatttggc
     9361 accgtgtttc tgggtaaaca aacgaaaagc gtcgagcgtc tcttcactaa catggtgctc
     9421 catgccttcc gcgtcgcgac gggcaatttc cggactgacg cccaacacca acaagaaatt
     9481 ttcgactatc tgatgtcgct cgcggctttc ctgcgccagc ttctctcctt ctgccgttaa
     9541 aaacacgcca cgccagggga tcatttcaat cagccccatg gttgccagcc gcttaagcat
     9601 tttagccacc gtcggttgcg aaactcccaa acgagcagcc atgtctacct gacgagcttc
     9661 ccccacttcc ctgatcaagt cagaaatcag ctcaacgtaa tcatcaataa gctcgcgccg
     9721 atgcgcctct ctgacctggc ggaacccttc aacgtgctct tccacgttca ctaactgcgt
     9781 cacttttttt gctgttggcg tacctgcgcg acgactcatt gtgcttcctc attatggtga
     9841 cgcataccta gcgttctcat ttcgtgcgca cattgtaaac cagagttgcg aaggtacaaa
     9901 aaattaacgt tttagcaata gctatataat atagcctgtg ctatatctgt atgtaatgca
     9961 atcatccctc aaggatcgac gggattagca agtcaggagg tcttatgaat gagttcaaga
    10021 ggtgtatgcg cgtgtttagt cattctccct ttaaagtacg gttaatgctg ctctctatgt
    10081 tgtgcgatat ggtcaacaac aaaccgcagc aagataaacc ttccgataaa tagcggcgtc
    10141 gcggtacgcc gcttcactcc tgcttttatg caggtacaac gcgttttgcc ctgaaaaacc
    10201 ccactttttg tcggatctgc aatccccttc gcaaaagatt tgttcgtcag tagttgacct
    10261 gaacggcagc tcgctctatc ttcttgcagc cctgcgtata ttgcggctcg cggatgcgga
    10321 cccttttccc ctcttcacgc actcttgcag gtattgaccc ttgacgccag ggtaagcaca
    10381 tggcgtttgt tacgatagtg gcatatgaat ttaaccctca aagaatcgct tgttacccgt
    10441 agccgggtat ttagcccgtg gactgcgttc tactttttac agtcgctatt aattaacctc
    10501 ggcttaggtt accccttcag tttgctctac accgctgcgt ttacggctat tttgcttttg
    10561 ctatggcgaa cattgcctcg cgtacaaaaa gttctggtcg gtatcagttc gctggtggcg
    10621 gcttgttatt tcccttttgc tcaggcctac ggcgcgccta acttcaatac attgctggca
    10681 ttgcactcca ccaatatgga agagtcgacc gaaatcctga cgatttttcc gtggtacagc
    10741 tacctggtcg gcatatttat ttttgcgctc ggcgtaatag caatcaggcg aaaaaaagag
    10801 agtgaaaaag cgcgctggaa taccttcgac agcctgtgcc tggtattcag tgcggcgaca
    10861 ttttttgttg ctcccgtgca aaacctggcc tggggcggcg tatttaaact gaaagatact
    10921 ggctatccgg tatttcgttt tgctaaggat gtcatcgtca ataataacga ggtgattgaa
    10981 gagcaagaac ggatggcaaa actttccgga atgaaagata cctggacggt cactgccgtt
    11041 aagccgaagt atcagaccta tgtggtggtg atcggagaaa gcgcgcgtcg cgatgccctc
    11101 ggtgcctttg gcggtcactg ggacaatacc ccgtttgcca gcagcgttaa cggtttgata
    11161 tttgctgact acattgccgc cagtggctcc acgcagaaat cgcttggctt aacgctcaat
    11221 cgcgttgtcg atggcaaacc acagtttcag gataactttg tcaccctggc aaatcgcgcg
    11281 ggcttccaga cctggtggtt ttccaaccag ggccaaatcg gcgaatacga taccgccatc
    11341 gccagcatcg ccaaacgtgc agatgaagtg tacttcctga aagaaggtaa ttttgaagca
    11401 gataaaaaca cgaaagacga agcgttactg gatatgaccg ctcaagtgct ggcgcaagag
    11461 cactcgcaac cgcagctgat tgttttacat ctgatgggct cacatccgca ggcctgcgac
    11521 cggacacaag gcaaatacga aacctttgtg caatcgaaag aaacgtcgtg ctatctctat
    11581 accatgacgc aaacagacga tttactgcgt aagctgtacg atcagttacg caacagcggc
    11641 agtagcttct cgctggttta cttttccgac cacggtctgg cctttaaaga gcgcggcaaa
    11701 gacgtgcaat accttgccca tgatgataag tatcagcaaa atttccaggt gccttttatg
    11761 gtcatttcca gcgacgataa agcgcatcgg gtgattaaag cccgccgctc ggccaatgac
    11821 ttcttaggct ttttctcgca gtggacggga attaaagcga aggaaataaa tatcaaatac
    11881 ccgtttatat ctgagaagaa agccgggccg atatacatca ccaacttcca gttacagaag
    11941 gtggattaca accatctcgg aaccgatatt ttcgacccga aaccttaaaa caaaaaaatc
    12001 cgccccgaga ggcggatttt ttatatcacc gaagtgatta gaagcggtaa ccaacaccgg
    12061 caatccaggt gcctacgtca acgctacgaa tacggctctg ctcgtaagag aagtccagag
    12121 caacgttttc catcgggttg aactgcagac cagcaccgta ggagaaaccg tagtcgctgg
    12181 tgtcgtgttt gtaggtcggg tattcagtgg tctggaattt accataaccc acacccacta
    12241 caccgtagat gcttgcccag tcgttaatgc ggtaagccgg accagcagtg atgccgtagt
    12301 actggttttt gttgtagtca ccagagcttg cagtacggct tttctcggtg taagtgaaag
    12361 aaccgatcac acccagcggg ctgttgtctt cttcatagcg gtatttcagg ttgaaaccgc
    12421 ccattttgtt catttggccc tgagcgtcgc tctgtgcgta accgccagtt acagtagaag
    12481 tcgcagctac ggaagtacct gcggtgaaag ccagaactgc ggccagtgct gaaagacatg
    12541 caattttttt cataaccacc tcaaatgtga ttcaaataag tcctaagttt taaatatatc
    12601 aaaaattaat gggaaactct tcgcgattta tgatgtctaa cgggccattt catgtaacag
    12661 aacgtttcca tacaccgcta tccatctaaa tttaaatcac tttttcagag aactgcgtaa
    12721 gtattacgca tgttttccct gtcattcatc cagattattc ctaatcacca gactaatgat
    12781 tccatcaatc ctggcgcatt ttagtcaaaa cgggggaaaa ttttttcaac aaatgctcaa
    12841 ccagcattgg gtatatccag tacactccac gctttactta ggtttagata tttgtgggag
    12901 aaaggatgcc tggttcatta cgtaaaatgc cggtctggtt accaatagtc atattgctcg
    12961 ttgccatggc gtctattcag ggtggagcct cgttagctaa gtcacttttt cctctggtgg
    13021 gcgcaccggg ggtcactgcg ctgcgtcttg cattaggcac gctgatcctc atcgcgttct
    13081 ttaagccatg gcgactgcgc tttgccaaag agcaacgatt accgctgttg ttttacggcg
    13141 tttcgctggg tggaatgaat tatctttttt atctttctat tcagacggta ccgctgggta
    13201 ttgcggtggc gctggagttc accggaccac tggcggtggc actgttctct tctcgtcgcc
    13261 cggtagattt cgtctgggtt gtgctggcgg ttcttggtct gtggttcctg ctaccgctgg
    13321 ggcaagacgt ttcccatgtc gatttaaccg gctgtgcgct ggcactgggg gccggggctt
    13381 gttgggctat ttacatttta agtgggcaac gcgctggagc ggaacatggt cctgcgacgg
    13441 tggcaattgg ttcgttgatt gcagcgttaa ttttcgtgcc aattggagcg cttcaggctg
    13501 gtgaagcact ctggcactgg tcggttattc cattgggtct ggctgtcgct attctctcga
    13561 ccgctctgcc ttattcgctg gaaatgattg ccctcacccg tttgccaaca cgcacatttg
    13621 gtacgctaat gagcatggaa cctgcgctgg ctgccgtttc cgggatgatt ttcctcggag
    13681 aaacactgac acccatacag ctgctggcgc tcggcgctat catcgccgct tcaatgggtt
    13741 ctacgctgac agtacgcaaa gagagaaaaa taaaagaatt agacataaat taaatttaca
    13801 tttctgcatg gttatgcata accatgcaga ttttctcgct acttttcctc tacatcgtct
    13861 ttatatatcg aattatgcaa aagcatattt attccgaaaa ttcctggcga gcaaataaat
    13921 aagaattgtt cttattaata tatctaactc attgagtttt tattggtttt gttttttcac
    13981 gcttgttacc actattaatg tgataggaac agccagaata gcggaacaca tagctggtgc
    14041 tatacttagt ctcgttaatt actgggacat aacatcaaga ggatatgaaa ttatgagtac
    14101 cgctaaatta gttaaatcaa aagcgaccaa tctgctttat acccgcaacg atgtctctga
    14161 cagcgagaaa aaagcaacag tagagttgct gaatcgccag gttatccagt ttattgatct
    14221 ttctttgatt accaaacaag cgcactggaa catgcgcggc gctaacttca ttgccgtaca
    14281 tgaaatgctg gatggcttcc gcactgcact gatcgatcat ctggatacca tggcagaacg
    14341 tgcagtgcaa ctgggcggtg tagctctggg gaccactcaa gttatcaaca gcaaaactcc
    14401 gctgaaaagt tacccgctgg acatccacaa cgttcaggat cacctgaaag agctggctga
    14461 ccgttacgca atcgtcgcta atgacgtacg caaagcgatt ggcgaagcga aagatgacga
    14521 caccgcagat atcctgaccg ccgcgtctcg cgacctggat aaattcctgt ggtttatcga
    14581 gtctaacatc gaataaatcc atcgctgatg gtgcagaact ttagtacacg ataaaagcgg
    14641 cttcctgaca ggaggccgtt ttgttttgta gcccgcctca acgcacttat ttagtgcatc
    14701 catctgctat caccagctgg ttaagtaaat tttttgtatc cacatcatca cacaatcgtt
    14761 acataaagat tgttttttca tcaggtttta cgctaaataa tcactgtgtt gagtgcacaa
    14821 ttttagcgca ccagtttggc gccccagaat ggtgcatctt caggctattg ccctataaat
    14881 cgtgcatcac gtttttgccg catctcgaaa aatcaatgag ttgcaaaact ggcacgattt
    14941 tttcatatat gtgaatgtca cgcaggggat cgtcccgtgg atagaaaaaa ggaaatgcta
    15001 tgaagtctgt attaaaagtt tcactggctg cactgaccct ggcttttgcg gtttcttctc
    15061 atgccgcgga taaaaaatta gttgtcgcga cggataccgc cttcgttccg tttgaattta
    15121 aacagggcga taaatacgtg ggctttgacg ttgatctgtg ggctgccatc gctaaagaac
    15181 tgaagctgga ttacgaactg aagccgatgg atttcagtgg gatcattccg gcactgcaaa
    15241 ccaaaaacgt cgatctggcg ctggcgggca ttaccatcac cgacgagcgt aaaaaagcga
    15301 tcgatttctc tgacggctac tacaaaagcg gcctgttagt gatggtgaaa gctaacaata
    15361 acgatgtgaa aagcgtgaaa gatctcgacg ggaaagtggt tgctgtgaag agcggcactg
    15421 gctccgttga ttacgcgaaa gcaaacatca aaactaaaga tctgcgtcag ttcccgaaca
    15481 tcgataacgc ctatatggaa ctgggcacca accgcgcaga cgccgttctg cacgatacgc
    15541 caaacattct gtacttcatc aaaaccgccg gtaacggtca gttcaaagcg gtaggtgact
    15601 ctctggaagc acagcaatac ggtattgcgt tcccgaaagg tagcgacgag ctgcgtgaca
    15661 aagtcaacgg cgcgttgaaa accctgcgcg agaacggaac ttacaacgaa atctacaaaa
    15721 aatggttcgg tactgaaccg aaataataac gctgcgcctg taaagcgcac tggcagttcc
    15781 ctctccccgc cggggagagg attaggggga agggcgcaaa cccgctccgg ggccattaat
    15841 taccctgaat ttgattattt acaccacggt aacaggaaca acatatgcag tttgactgga
    15901 gtgccatctg gcctgccatt ccgcttctga ttgaaggtgc caaaatgacc ctgtggattt
    15961 cggtcctcgg tctggctggc ggtctggtga tcggattgtt ggcaggtttt gcacgcacct
    16021 tcggtggttg gatagccaac cacgtcgcgc tggtctttat tgaagtgatc cgcggcacac
    16081 ctatcgtcgt ccaggtgatg tttatctatt tcgccctgcc gatggcgttt aacgatttac
    16141 gcatcgaccc gtttactgcg gcggtggtca ccatcatgat caactccggc gcgtacattg
    16201 cggaaatcac gcgtggtgcg gtgctgtcta ttcacaaagg ttttcgtgaa gcaggactgg
    16261 cgctcggtct ttcacgttgg gaaaccattc gctacgtcat tttaccgctg gcactgcgtc
    16321 gtatgctgcc gccgctgggt aaccagtgga tcatcagcat taaagacact tcgctgttta
    16381 ttgttatcgg cgtggctgaa ctgacccgtc aggggcaaga aattattgcc ggtaacttcc
    16441 gcgcccttga gatctggagc gccgtggcgg tgttctatct gattattacc ctggtgctga
    16501 gctttattct gcgtcgtctg gaaagaagga tgaaaatcct gtgattgaat ttaaaaacgt
    16561 ctccaagcat tttggtccaa cccaggtgct gcacaatatc gatttgaaca ttgctcaggg
    16621 cgaagtcgtg gtgattatcg ggccgtccgg ttccggtaaa tcgaccctgc tgcgctgcat
    16681 caacaaactg gaagaaatca cctccggcga tctgattgtc gatggcctga aggttaacga
    16741 tccgaaagtt gatgagcgcc tgattcgcca ggaagcgggt atggtgttcc agcagtttta
    16801 cctcttcccg catctgacgg cgctggaaaa cgtcatgttt ggcccgctac gcgtgcgtgg
    16861 cgcgaataaa gaagaggcgg aaaaactggc gcgtgagctg ctggcgaaag tcggtctggc
    16921 agagcgtgca catcactacc cttccgaact ttctggtggt caacagcagc gtgtggcgat
    16981 tgcccgcgcg ctggcggtga agccgaaaat gatgctgttt gatgaaccga cttccgctct
    17041 tgacccggaa ctgcgccatg aagtgctgaa ggttatgcag gatctggctg aagaagggat
    17101 gacgatggtg atcgtgaccc acgaaatcgg ttttgccgag aaagtagctt cgcgcctgat
    17161 ctttatcgac aaaggccgga ttgcagaaga tggcgatccg caggtgttga tcaagaaccc
    17221 gccgagccag cgcttgcagg aatttttgca gcacgtctct taacttctct gcctcccttt
    17281 cccccgaaag ggaggcacac cagattcctc tcatttaaaa tcgcccagcc tccagcatct
    17341 atacttatct ttttgctctg ttttctcact ggaggagtca tgcggtggat cctgttcatc
    17401 ctcttctgcc tgttgggcgc acctgcccac gcggtatcca tacccggcgt tacaaccaca
    17461 acgacaacgg actcaacgac tgaaccggcc ccggaaccgg atatcgaaca aaaaaaagcg
    17521 gcctatggcg cactggcgga tgtgctggat aatgacacct cgcgtaaaga gttgatcgac
    17581 cagttgcgca ccgtcgccgc gacgccccct gcggaacccg taccgaagat cgtgccgccg
    17641 acgctggttg aagagcaaac cgtgctgcaa aaagtcaccg aagtcagccg ccattatggt
    17701 gaagcccttt ccgcccgctt cggacaactt tatcgcaata tcaccggctc cccgcataag
    17761 ccgtttaatc cacaaacctt cagcaatgcg ttgacccatt tttcaatgtt agcggtatta
    17821 gtgtttggtt tttactggct gattcgcctg tgcgcacttc cgctgtatcg caaaatgggc
    17881 cagtgggcgc ggcaaaaaaa tcgtgagcgc agtaactggt tgcagcttcc ggcgatgatt
    17941 atcggggcgt ttattatcga cctgctgtta ctggcactga cattatttgt cggtcaggtt
    18001 ttaagcgaca acctgaatgc gggcagtcgc accatcgctt tccaacaaag tttgtttctc
    18061 aacgcctttg ccctgattga atttttcaaa gccgtactac gcctgatttt ttgcccaaac
    18121 gtggctgaac tgcgcccgtt cacgattcat gacgagaccg cccgttactg gagtcgtcgc
    18181 ctgagctggt taagcagcct gattggctat ggtctgattg tggctgtgcc gattatctct
    18241 aatcaggtga atgtacagat aggtgcgctg gcgaacgtca tcattatgct gtgcatgacc
    18301 gtctgggcgt tgtacctgat ttttcgtaat aaaaaagaga tcacccagca tttgctcaac
    18361 tttgcggagc attcgctggc ctttttcagc ctgtttatcc gcgcctttgc gctggtgtgg
    18421 cactggctgg caagcgctta ttttatcgtg ctgtttttct tttcgttgtt cgatccgggc
    18481 aacagcctga aatttatgat gggtgcaacg gtgcgcagcc tggcgattat tggtatcgca
    18541 gcatttgttt ccggtatgtt ttcccgctgg ctggcgaaaa ccatcaccct ctcgccacat
    18601 actcagcgta actatcccga gctgcaaaaa cggctgaatg gctggctttc ggcggcactg
    18661 aaaacagcgc gtattctgac agtctgcgtg gcggtaatgc tgctgttgag cgcatgggga
    18721 ttgttcgatt tctggaactg gctgcaaaac ggcgcggggc agaaaaccgt agatatcctg
    18781 atccgtatcg cactcattct tttcttctcg gcggttggct ggacggtgct cgccagtttg
    18841 atagaaaacc ggctggcttc ggatattcat ggccgcccgc tacccagcgc ccgtacgcgt
    18901 accctgctga cgctgtttcg taacgcgctg gcggtgatta tcagtaccat caccatcatg
    18961 attgtgttgt cggaaatcgg cgtcaatatc gcgccattgc tggcaggtgc cggggcatta
    19021 ggtctggcta tctcgtttgg ttcgcaaacg ctggtgaaag atattatcac cggggtattt
    19081 attcagtttg aaaacggcat gaacactgga gatttggtga ctatcgggcc gttgaccggc
    19141 acagtggaac ggatgtcgat tcgctccgtg ggcgtgcgcc aggataccgg ggcgtatcac
    19201 atcattccgt ggtcttcgat aaccaccttt gctaacttcg tccgcggtat tggttcggta
    19261 gtggcaaact acgatgttga tcgccatgaa gatgctgata aagccaatca ggcactgaaa
    19321 gatgcggtaa cggaattgat ggaaaacgaa gatattcgcg ggctgattat tggtgaaccg
    19381 aactttgccg ggattgtcgg cttaagcaat accgcgttta cactgcgtgt ttcgttcacc
    19441 acgctgccac tcaaacagtg gacggtacgc tttgccctcg acagccaggt gaaaaaacat
    19501 ttcgacctgg cgggcgttcg cgcgccagtg cagacttatc aggtgctgcc tgctccgggc
    19561 gcgcccccgg ctgaaccgct gccgccgggg gagccaacgc tttaacgttg gcgattaaca
    19621 aaacggcggc gctgctcgtc gttcataaag gtccaggcaa taaagcgact ctgcttttgc
    19681 ccctgggcca tctctttttt aaccaccttc accgcgccca cgtccgtcag agcacgatac
    19741 aacggcggta agttttcacc acgagatacc agcgaggtaa accacatcac ctgcttcgcg
    19801 aagcctttgc tctcttcaat catctttttg ataaaggcga cttcaccgcc ttcacaccac
    19861 aactcctgtt gctgaccacc aaagttcagt gcatcgtctt tgttcagccc caggttacgg
    19921 cgtttacgct cactacctgc ccgcgccgca gcggcggaat cgtggaatgg cgggttacac
    19981 aaggtcgcgt cgtattgctc gtttttatgg ataatgccgt taaaaatcgc cccactctct
    20041 ttttgccgac gcagacgaat ggcgcggtta agccccggat tagcactgat gatcgcctgc
    20101 gcactgctta acgcctggct gctggtttca ctaccggtaa aacgccagcc atattcatgt
    20161 acgccaatca gcggataaat acagttcgca ccaacgccga tatccagaat gctggcatta
    20221 gctggaattg ttccgcttgc ttcggccagt aaatcggcaa ggtgatgaat ataatccgcc
    20281 cggcccggta ctggtgggca gagaaaaccg tccgggatat cccagttcgc tacggcgtaa
    20341 aaatgggcca gcaacgcctt attgagcgcc ttcaccgcca gcggattggc aaagtctacg
    20401 ctttgctccc cggcgggtgt aagcgtgagg aattgcctga gttcaggatt gacctgacaa
    20461 agcgtggcga gatcgtagcg gctgtgatga cggttgcgcg gatgcaaccc cggtttctgg
    20521 gcggacatag cattctcctt taagcagcgg cgtaagatac ccgcataacg cgacagggta
    20581 aatatctggc tgcgtttatc atttctaaaa accttcacat ggctatggtt tatggcaatt
    20641 caccataaaa atcatcatca tgcatgtcat tgatttgtct tattttgtaa aaatatatcg
    20701 ttcaaaaaat aagcgcatct ctgtcaattt tttgcgtcat tttcatcgcg caggcgacct
    20761 acacttatct ggcactaccc gttgtattta tgaggatgtc attatgaaaa agtgcctcac
    20821 actactgatt gccaccgtcc tgagcggaat ctctctcacg gcttatgccg cgcaaccgat
    20881 gagtaacctg gacagcggtc agctacggcc cgccggtacc gtttcggcaa ctggcgcatc
    20941 aaacctaagc gatctggagg ataaactggc agaaaaagcg cgcgagcaag gcgcgaaagg
    21001 ttatgtcatt aattccgcag gcggaaatga ccagatgttc ggtactgcaa tcatctacaa
    21061 ataaccccac caacgtaact gcattcgcct ctggatgcag ttttcgtcca atttgtaagc
    21121 aaaaaacatc aaatatgaac gcaatgtaaa taaatgtatt tctttttcgc gcaatgggtg
    21181 atagaaaatc gcgccaagtg ataatgctta tcaaaattat tatcacttgc acggactcta
    21241 tcacgggatt aacagtggca tcgcatccgc agagatgctt tctcgtgaca gtgaaaattt
    21301 caccatataa gaaaaagtca cctgcaaaat ggaaaacaat cgcaatttcc ctgccagaca
    21361 atttcattcg ctcacgttct ttgccggtct ttgtattggt attacgcctg tggcgcaggc
    21421 actcgctgcc gaagggcaag ctaacgcgga tgacacgctg gttgtcgaag catcaacgcc
    21481 ttcgctttat gcgccacaac aatctgccga tccgaaattc tcgcgtccgg tagcggatac
    21541 tacccgcacg atgacggtga tttctgaaca agtgattaaa gatcagggtg caaccaacct
    21601 taccgacgcg ctcaaaaacg tccccggcgt gggtgcgttt tttgcgggtg agaacggtaa
    21661 ctccaccact ggcgacgcca tttatatgcg cggtgccgat acctctaaca gtatttatat
    21721 cgatggcatt cgcgatatcg gcagcgtctc gcgcgacacc ttcaataccg agcaggtcga
    21781 agtaattaaa gggccgtccg gcaccgacta cgggcgcagc gcgccgacag gctcgatcaa
    21841 tatgatcagc aaacagccgc gcaatgattc cggcattgac gcctccgcca gtattggcag
    21901 cgcctggttc cgccgcggca cgctggacgt caatcaggtc attggtgata ccactgcggt
    21961 gcgcctgaat gtaatgggcg aaaaaacgca cgatgccgga cgcgacaaag tcaaaaatga
    22021 gcgttacggc gtcgcccctt ctgtcgcttt tggccttggt acagcgaatc gtttgtatct
    22081 taattatctg catgtcaccc agcacaacac gccagacggc ggcattccga ctatcggttt
    22141 accgggttat tctgccccat ctgcgggaac agcagccctg aatcattccg gaaaagttga
    22201 tactcataac ttttacggca cagattccga ttacgacgat tcgaccaccg acaccgctac
    22261 catgcgtttt gagcacgaca tcaacgataa caccaccatt cgcaacacta cgcgttggtc
    22321 gcgcgtaaag caggattacc tgatgacggc gattatgggt ggagcgtcga atattaccca
    22381 gcccaccagc gatgtgaata gctggacgtg gtcacgcacg gcgaatacca aagatgtgag
    22441 taacaaaatt ctcaccaacc agaccaacct gacctcgacg ttctataccg gcgctatcgg
    22501 tcatgatgtc agtaccggcg tggaatttac ccgcgaaaca caaactaact atggcgttaa
    22561 tccggtgacg ttaccggcgg taaatattta tcatcctgac agcagcattc atcccggcgg
    22621 cctgacgcgc aacggcgcaa acgccaatgg tcagacggat accttcgcaa tttatgcctt
    22681 cgatacgctg caaatcaccc gtgattttga gctgaacggc gggatccgtc tggataatta
    22741 tcatactgaa tatgacagtg ctaccgcctg cggcggcagc gggcgcggtg ccatcacctg
    22801 ccctgctggc gtggcaaaag gttctccggt caccaccgtc gacaccgcca agtcgggcaa
    22861 tctggtgaac tggaaagccg gggcgctgta tcacctgacg gaaaacggca atgtctatat
    22921 taactatgcc gtttcccagc agccgccggg cggcaacaac ttcgcccttg cgcagtctgg
    22981 cagcggtaac agtgccaacc gcaccgattt taaaccacaa aaagccaaca ccagcgagat
    23041 tggcaccaaa tggcaggttc tggataaacg cctgctactt accgccgcgc tgttccgtac
    23101 tgatattgaa aatgaagttg agcaaaatga tgacgggact tattcgcaat atggtaagaa
    23161 acgtgtcgaa ggctacgaga tatccgtggc cgggaatatc acccccgcat ggcagatgat
    23221 tggcggctat acccagcaaa aagcaaccat caaaaacggc aaagatgttg cccaggatgg
    23281 ttcctcatcg ctgccgtata ccccggaaca cgccttcacc ttatggagcc agtatcaggc
    23341 aaccgatgat atttctgttg gtgcgggcgc acggtatatc ggcagtatgc ataaaggttc
    23401 agacggcgcg gtggggacgc cagcatttac cgaaggttat tgggtcgccg acgccaaact
    23461 ggggtatcgg gttaatcgca atctcgactt ccagctaaac gtctacaacc tgtttgatac
    23521 cgattacgtc gcctcaatca ataagagcgg ctaccgttat cacccgggcg agccaagaac
    23581 cttcttgctc acagccaata tgcatttctg attcagatgt ggggcgcaag gctcactaat
    23641 gttgatggcg agagcaggac tgcctgatgc gctgcgctta tcaggcctac aaggttgtgc
    23701 aatacactga attcacatca tttggtaggc cggataaggc gttcacgccg catccggcac
    23761 ctgagcacca ttgcctgctg ttaaaaatcg gtgtggggcg caggccccac tttttggaga
    23821 acttgtatga tgtaccacat tcccggcgtg ttatcgccac aggacgtcgc ccgttttcgc
    23881 gaacacctgg aacaagccga atgggtggat ggacgcgtca ccaccggcgc acaaggtgcg
    23941 caagttaaga acaatcaaca agtagacacc cgcagcgccc tgtacgccgc cctacaaaat
    24001 gaggtgctga acgcggttaa ccaacatgct ttgttctttg ccgcggcctt gccgcgtacc
    24061 ctttccacgc cgctgtttaa tcgctatcag aacaatgaaa cctatggttt tcatgtggat
    24121 ggcgcagtac gcagccatcc acaaaacggc tggatgcgca ctgacctttc tgccacgctg
    24181 tttttaagcg atccagaaag ctacgacggc ggcgaactgg tcgttaatga caccttcggg
    24241 caacatcggg taaaactccc ggcaggcgat ctggtgttgt atccctccag cagcctgcat
    24301 tgcgtgacac ccgtaacccg cggcgtacga gtggcatcat ttatgtggat ccagtcgatg
    24361 atccgtgatg ataaaaagcg cgccatgctg tttgaactgg acaacaatat tcagtcgctg
    24421 aaaagccact acggcgaaag tgaagagatc ctgtcgctgc tcaatcttta tcataatctg
    24481 ctgcgggaat ggtcggagat ctgacgcttg aactgcccga gttcgtccct atattagcta
    24541 cacttaactg tacaagtatt gatatgggga ggacgatatg gcatccggtt gggctaatga
    24601 tgacgccgtc aacgaacaga tcaacagtac aattgaagat gcgattgccc gcgctcgggg
    24661 tgaaattccg cgtggcgaaa gcctggatga atgtgaagag tgcggagccc tcatcccgca
    24721 agcccgtcgg gaagccattc ctggcgtgcg cttatgcatt cattgtcagc aggagaaaga
    24781 tttacaaaaa ccagcttata caggatataa tcgcagaggt tcgaaagaca gccagttacg
    24841 ttaactacaa ctggcgggat gtatagcgag tataatttgt attttgcgtg gcattccgtg
    24901 cctttaacgc tgtcgtgact gccacgctgc ataaaacaag tccataagtt caacaaataa
    24961 atgaattatc attatgttag atatcaatca atttatttga acaaggtggt caattctctt
    25021 cgattttatc tctcgtaaaa aaacgtgata ctcatcacat cgacgaaaca acgtcactta
    25081 tacaaaaatc acctgcgaga gattaattat gaaaactatc aatactgttg ttgctgctat
    25141 ggctctttca actctgtcat ttggcgtgtt cgcggcggaa ccggtaacgg catcccaggc
    25201 acagaacatg aataaaatcg gcgtggtttc tgccgatggc gcatccaccc tcgatgccct
    25261 ggaagcgaaa ctggctgaga aagccgcggc ggccggtgcc agtggataca gcattacttc
    25321 cgccaccaac aacaataaat taagcggtac tgcggtaatt tataagtaat tcgtaggacg
    25381 gataaggcgt ttacgccgca tccggcaaca gatgcctgat gcgacgctac cgcgtcttat
    25441 cagacctaca aaatccgaac cgttggctgg atcaggcgtt tacgctacgt tcagcacaag
    25501 tatttccctg tctgtttgcc gacagacgca tatgctctaa ccctcattga tcctatgtta
    25561 cccttgtttg cccgtccgcc actggacggg ctttttttag ctggctaact gctgacagaa
    25621 agcctgcaac gtctcttccg gcataccaat ctgccgcgct gcatcacaaa tggcctgcca
    25681 gcttcccgca tccagtggaa tcccctgctc ctgccgttcg cgacgcgtgt tcacttccca
    25741 ctcgcccggt agcaaaatcg gcttatcgtc atcatgcggc gaggctttca cccactcggc
    25801 aaaggcttcg gtctgtgcac tacaatccgg cgcgccgaac agttccgggt tgataatgat
    25861 agtggtcatg cagttgagaa tggcatcggg actggtttgt aacgtttcct gatgcgtcgt
    25921 tttaccgcca gaaagcgccc cgccaagaat ttcacacatc gctgcaaggg cgtagccttt
    25981 atgttcggca aaggtcaaca gcgaacccaa cggcgactcc tgcattaccg ccggattggt
    26041 cgtcggcacg ccgttaacgt caatcaggca acctggcggc acggggacgc ctttatgcca
    26101 ggcgacgcgg gttttgccaa atgcaatggc gctggtggcg taatcgagca acagcgggaa
    26161 attatcttta cgagggaaaa ccacacagaa cggattggtg ccaaagcggc tgtcgcgacc
    26221 gtggaacggc gcgaccattg gaataccgac cacgctaaca aagtggatag agacaaaccc
    26281 cgccgctgca cactgctccg cccagtaacc gatacggccg atatgatgcg agttatggag
    26341 cgccacggcg gcaatgccgt gctgatgcgc tttctcaatc cccagcgcca tcgcttcgtg
    26401 tgccgcgacc tgaccaaatg cgcgatcgcc atcgagcgtg accgccgccc ccgcctcttt
    26461 aacgactttg gcatgatggt taatttgcag atgcccctga ctccaggagc gcacatagct
    26521 tgggatcatg ccaataccat gtgaatcatg ccctgccagg tttgccgcga ttaaatgatc
    26581 ggcgactaat ttcgcttctt gttcctcact acccatctga cgaaatacag cctgaataaa
    26641 actgtgcagc gtctgagcat caaagcgatg accacttacc atggttaact cctggtgtgt
    26701 ttattttgtg gtttactctt cccccgaagg ctctgacggg aaccatgcag cctgataact
    26761 ccgggggtta caagcccgtt
//
LOCUS       DAZFJL010000062        26318 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFJL010000062 DAZFJL010000000
VERSION     DAZFJL010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.062"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            14..166
                     /gene="hokA"
                     /locus_tag="ACHJ8C_002464"
     CDS             14..166
                     /gene="hokA"
                     /locus_tag="ACHJ8C_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO5552308.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKKENYELAAFL
                     ACKLKE"
     gene            complement(143..214)
                     /locus_tag="ACHJ8C_002465"
     CDS             complement(143..214)
                     /locus_tag="ACHJ8C_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5552309.1"
                     /translation="MRNAVRKVGITSRRRLLLFQFAG"
     gene            complement(354..566)
                     /gene="cspA"
                     /locus_tag="ACHJ8C_002466"
     CDS             complement(354..566)
                     /gene="cspA"
                     /locus_tag="ACHJ8C_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO5552310.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(847..1137)
                     /gene="yiaG"
                     /locus_tag="ACHJ8C_002467"
     CDS             complement(847..1137)
                     /gene="yiaG"
                     /locus_tag="ACHJ8C_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO5552311.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1571..2281
                     /gene="yiaF"
                     /locus_tag="ACHJ8C_002468"
     CDS             1571..2281
                     /gene="yiaF"
                     /locus_tag="ACHJ8C_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO5552312.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2331..3305)
                     /gene="ghrB"
                     /locus_tag="ACHJ8C_002469"
     CDS             complement(2331..3305)
                     /gene="ghrB"
                     /locus_tag="ACHJ8C_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO5552313.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3409..4068)
                     /gene="yiaD"
                     /locus_tag="ACHJ8C_002470"
     CDS             complement(3409..4068)
                     /gene="yiaD"
                     /locus_tag="ACHJ8C_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO5552314.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4221..6554
                     /gene="bisC"
                     /locus_tag="ACHJ8C_002471"
     CDS             4221..6554
                     /gene="bisC"
                     /locus_tag="ACHJ8C_002471"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO5552315.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETIDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMLVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFADLSERWEKGGYTRFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLAP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQTRGIQD
                     GDTVRLWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6523..6963)
                     /gene="yiaC"
                     /locus_tag="ACHJ8C_002472"
     CDS             complement(6523..6963)
                     /gene="yiaC"
                     /locus_tag="ACHJ8C_002472"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO5552316.1"
                     /translation="MIREVQHSELPAILELWLESTTWGHPFIKSSYWRDCIPLVRDAY
                     LANAQNWVWDEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYHAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6960..7523)
                     /gene="tag"
                     /locus_tag="ACHJ8C_002473"
     CDS             complement(6960..7523)
                     /gene="tag"
                     /locus_tag="ACHJ8C_002473"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO5552317.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDIERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCHPGNKP"
     gene            7681..8379
                     /gene="yhjY"
                     /locus_tag="ACHJ8C_002474"
     CDS             7681..8379
                     /gene="yhjY"
                     /locus_tag="ACHJ8C_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO5552318.1"
                     /translation="MIIKKSGGRWQLSLLASAVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8608..9816
                     /gene="yhjX"
                     /locus_tag="ACHJ8C_002475"
     CDS             8608..9816
                     /gene="yhjX"
                     /locus_tag="ACHJ8C_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5552319.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLGAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTHLLE
                     TVGLEKTFVIWGAIVLVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDAISAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVIALVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10140..11831
                     /gene="eptB"
                     /locus_tag="ACHJ8C_002476"
     CDS             10140..11831
                     /gene="eptB"
                     /locus_tag="ACHJ8C_002476"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO5552320.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAREAAAN"
     gene            11923..11999
                     /locus_tag="ACHJ8C_002477"
     tRNA            11923..11999
                     /locus_tag="ACHJ8C_002477"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11957..11959,aa:Pro,seq:cgg)
     gene            12743..14350
                     /gene="dppA"
                     /locus_tag="ACHJ8C_002478"
     CDS             12743..14350
                     /gene="dppA"
                     /locus_tag="ACHJ8C_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO5552321.1"
                     /translation="MRISLKKPGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPKIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14657..15676
                     /gene="dppB"
                     /locus_tag="ACHJ8C_002479"
     CDS             14657..15676
                     /gene="dppB"
                     /locus_tag="ACHJ8C_002479"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO5552322.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15686..16588
                     /gene="dppC"
                     /locus_tag="ACHJ8C_002480"
     CDS             15686..16588
                     /gene="dppC"
                     /locus_tag="ACHJ8C_002480"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO5552323.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16599..17582
                     /gene="dppD"
                     /locus_tag="ACHJ8C_002481"
     CDS             16599..17582
                     /gene="dppD"
                     /locus_tag="ACHJ8C_002481"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO5552324.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNLVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDKCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17579..18592
                     /gene="dppF"
                     /locus_tag="ACHJ8C_002482"
     CDS             17579..18592
                     /gene="dppF"
                     /locus_tag="ACHJ8C_002482"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO5552325.1"
                     /translation="MSTHEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLIGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQK
                     PAV"
     gene            18817..19140
                     /locus_tag="ACHJ8C_002483"
     CDS             18817..19140
                     /locus_tag="ACHJ8C_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000563102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5552326.1"
                     /translation="MICSGPRNLAQELKAIGDQLQELQKKLIQGPNISQPRPLLTIET
                     PRHLGEQLNARRKELGIDLYTLELQTGISTSTLKRLFKDPEQVKFGSVFAVANVLGVK
                     LCIDE"
     gene            19125..19436
                     /locus_tag="ACHJ8C_002484"
     CDS             19125..19436
                     /locus_tag="ACHJ8C_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HFO5552327.1"
                     /translation="MHRRVKVLLYGQVVGELSQNDSGFLFQYAQDYHGPAISISLPVA
                     QRQFPSETLHPYFASLAPEGWLRQRYSQIQHRDENDLLGMLIDNGKNLLGAIQILPWE
                     E"
     gene            19436..20443
                     /locus_tag="ACHJ8C_002485"
     CDS             19436..20443
                     /locus_tag="ACHJ8C_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HFO5552328.1"
                     /translation="MANCRILLTPLNERDEQRGYSTQGLKRLSGTAKLTPRLGFTRTQ
                     FVQELPRQQKGMSISGYQPKLQLVLDEGEFRVVDHQGNFILKPSPADFPGLAENEHAT
                     MTLMSRLGFDVPVHGLLSFAPQSEEELEYAFVIRRYDRDYKGLPVHQEQLDGAMQITD
                     KYGKTGNDNEQYVSYETLARFLVAHVNDNIAFKIDLFRRIVYAWLLGNNDMHLRNFGL
                     VYSDGLTPTLAPVYDFVSVAPYPEYFYSNYLALPLLTREEGGRELAPGFHSDYGEYIG
                     QDFLLLGESMGLAPRLLEKLFQDIRKENAIVMETYEQSFMTQDHIQAVLQCYRHRLGL
                     L"
     gene            complement(20460..21731)
                     /gene="yhjV"
                     /locus_tag="ACHJ8C_002486"
     CDS             complement(20460..21731)
                     /gene="yhjV"
                     /locus_tag="ACHJ8C_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5552329.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLFYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASDFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYVTLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTRKINSRVLTLAICTFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLISKV
                     SQLKKLRGLKTWLILFYGILLCFSPLLKLIE"
     gene            22207..22314
                     /gene="ldrD"
                     /locus_tag="ACHJ8C_002487"
     CDS             22207..22314
                     /gene="ldrD"
                     /locus_tag="ACHJ8C_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5552330.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(22400..24079)
                     /gene="bcsG"
                     /locus_tag="ACHJ8C_002488"
     CDS             complement(22400..24079)
                     /gene="bcsG"
                     /locus_tag="ACHJ8C_002488"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO5552331.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLFVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDIPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSIFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQSELMDQKNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFLGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(24076..24267)
                     /gene="bcsF"
                     /locus_tag="ACHJ8C_002489"
     CDS             complement(24076..24267)
                     /gene="bcsF"
                     /locus_tag="ACHJ8C_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO5552332.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(24264..25835)
                     /gene="bcsE"
                     /locus_tag="ACHJ8C_002490"
     CDS             complement(24264..25835)
                     /gene="bcsE"
                     /locus_tag="ACHJ8C_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO5552333.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPGKITLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINSGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGCWTLVQHQEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLAQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            26108..26296
                     /gene="bcsR"
                     /locus_tag="ACHJ8C_002491"
     CDS             26108..26296
                     /gene="bcsR"
                     /locus_tag="ACHJ8C_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO5552334.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT         6435 a         6412 c         6835 g         6636 t
ORIGIN      
        1 acagggggct gggatgccgc agaaatatag attactttct ttaatagtga tttgtttcac
       61 gcttttattt ttcacctgga tgataagaga ttcactgtgt gaattgcata ttaaaaagga
      121 gaattatgag ctggccgcgt ttttagcctg caaattgaaa gagtaagagt cttcggcggg
      181 aagttattcc cacctttctt acggcgttgc gcattctcat tacacccaaa tttattcttc
      241 acaaaaataa cactagattc tattacgcga tcgattattt atttcctgaa aaacaaataa
      301 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      361 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      421 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacacg
      481 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      541 acgataccag tcattttacc ggacatagtg tattaccttt aataattaat tgtgcctttc
      601 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      661 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      721 accgttgatg tgcattaagc cacgcattgg tgggtgatgc aacaattatt tttcatattt
      781 atgattaatc ggttggaaaa cggtcctgtt atcaggaccg taaacagaaa taaagtggat
      841 aaaagtctat tccatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      901 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
      961 tgatacgccc aaaacccggg caaaatcatc gatttttaat cctgtccctt ttcgtaactg
     1021 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1081 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1141 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1201 ggaaaaacag acacagcggg gtgtagtcag ggcatactct gactatgatt cgattcagcc
     1261 agtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttac
     1321 cactcccggt ttgtaaagat ttcgaaaatc taacgttctg cgaaggcgta aatctctggc
     1381 tacaaaaaag gcaacggcaa tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1441 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1501 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1561 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1621 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1681 tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1741 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1801 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gttgtagaca gcgttaacgc
     1861 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaacggttc
     1921 gctgggtgtg ctggcgcaac agctgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     1981 tgcgttaaaa cagagtgatg acctgaaacc ggtctttgat caggcattca ccaaagtggt
     2041 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2101 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2161 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2221 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2281 aagagtaaaa gcccgagcga ttatcgggct ttactccacg cagtcgcggc ttagtccgcg
     2341 acgtgcggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2401 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2461 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2521 acatccagcc cggcggcgtg aatttcccct ttctgcaatg cagcaatcag tgcattttca
     2581 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2641 tgttctgcgc caaacagatg atgcgtctca tccgttaacg gcaggatcag gcaaacgaaa
     2701 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2761 tctttatggt ggcgacgtgc gttatagagg atgggcatgt tgaagccaaa gtgcgcacgt
     2821 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2881 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     2941 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3001 gtttctgtta atacggttgg tgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3061 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3121 tccagcaatg cggcatcaac attctcattt gaacccagca agccttcagc ttcggcaaaa
     3181 attgctgcat tttgttcgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3241 tgttcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3301 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3361 aaactgaaga atgccaggtg atttctgcat cacctgacat gaaagggatt acagtgggct
     3421 taaggtaatt tctacacggc ggttttgcgc tttaccttct gcggtgctgt tgctggcgat
     3481 tgggttagcc gggccaaggc cttgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3541 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3601 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagtac
     3661 cattgccacg ccggtcaggg tgttagcgcc cgccggtttc agggttgcgc tgctgctgtc
     3721 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3781 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataaccaac
     3841 gccgccgccc agagctgcgc ctgctgctgc gccaatgagc gcgcctttac cgcgatcttt
     3901 cttcgaagaa gagagcgcac caatacccgc gccaacgaga gagcccagac ctgcgccgat
     3961 agcagattta cctgcttcgc gttcgccggt gtaagggtta gttgtacagc cagataccgc
     4021 cagagcgcca ctcactacgg cggcaataag ataaacacgt ttcttcattg ttaatcctta
     4081 ataacctttt tattctttgc cacgggttcc gtggcgggag attatgccgc gtgaacatga
     4141 agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc aatacatcta
     4201 acctttcagg agtcttcggt ttggccaact catcctcacg atattccgtt ctgactgccg
     4261 cccactgggg gcctatgctg gttgaaactg acggcgaaac cgtgtttagc tcgcgtggcg
     4321 cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag gttcacagca
     4381 atacgcgggt gcgatttcca atggtgcgca aaggctttct tgcgtcaccg gaaaatccgc
     4441 aaggcattcg tgggcaggat gaatttgttc gcgtgagttg ggatgaggcg ctggagctta
     4501 ttcaccatca acataaacgc attcgtgagg cttatggtcc ggcatcgatt tttgctggtt
     4561 cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta caacgctata
     4621 tggcgctggc aggcggttat accgggcatc tgggggatta ttcgaccggc gcggcacagg
     4681 cgatcatgcc gtatgtcgtg ggtggcagcg aagtttatca acagcaaacc agttggccgc
     4741 tggtgctgga acatagcgat gtcgtggtgc tgtggagtgc taacccactc aatacgctga
     4801 aaattgcgtg gaatgcatcc gatgagcagg ggctttctta cttttccgca ctgcgtgaca
     4861 gcgggaaaaa gctgatctgc attgatccaa tgcgatcgga aaccatcgat ttctttggcg
     4921 ataaaatgga gtgggtggca ccacacatgg gcaccgatgt tgcgttgatg ctggggatcg
     4981 cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctggcgcgt tgcaccacag
     5041 gttatgccgt cttcgcctct tatttgctgg gcgagagtga cggtatagcg aaaaacgccg
     5101 aatgggcggc agagatttgt ggtgttggcg cagcgaaaat ccgcgagctg gcggctattt
     5161 tccaccaaaa tacaaccatg ctgatggcag gctggggaat gcagcgccaa cagtttggtg
     5221 agcaaaaaca ctggatgctc gtcacgctgg cggcaatgtt ggggcaaatc ggcacacccg
     5281 gcggcggttt tggtctttct taccattttg ccaatggtgg taaccccacg cgccgcgctg
     5341 cggtgctctc ttccatgcag ggcagcttgc cgggtggcac cgatgcggta gataaaatcc
     5401 ctgtcgcccg cattgttgaa gcactggaaa accctggtgg cgcatatcaa cataacggta
     5461 tggaccgaca tttcccggat attcgtttta tctggtgggc gggtggtgcc aactttactc
     5521 atcatcagga taccaatcgc ctgatccgtg cctggcaaaa accggagctg gtggtgatct
     5581 ccgaatgctt ctggacggct gcggcgaaac acgcggatat cgttctgcct gcgacaacct
     5641 cttttgagcg taatgatctc accatgaccg gtgattacag taatcagcat ctggtgccga
     5701 tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga ttttgatgtt tttgccgact
     5761 tgagtgaacg ttgggagaag ggcggttata cacgttttac ggaaggaaaa agtgagctgc
     5821 aatggctgga aacgttttat aacgttgccc ggcagcgcgg ggcaagccag caggttgaat
     5881 tgccgccatt tgctgagttc tggcaagcca accagttaat tgagatgccg gaaaacccgg
     5941 acagtgagcg gtttattcgc ttcgccgatt ttcgccgcga tccgcaggcg catccattaa
     6001 aaaccgccag cggtaagatt gaaatcttct cgcagcgtat tgccgattac gcttatccgg
     6061 attgcccggg gcatccaatg tggctggcgc cggacgaatg gcagggcaat gccgaaccgg
     6121 aacagttgca ggtactttct gctcatccgg cgcatcgtct gcacagccag ctgaattaca
     6181 gttctctgcg cgaattgtac gcggtggcaa atcgtgagcc tgtcaccatt catcctgacg
     6241 atgcccagac gcgcggcata caagatggcg atactgttcg gctgtggaac tcacgcgggc
     6301 aaattcttgc cggagcggtc attagcgagg gaattaaacc tggcgtgatt tgtattcacg
     6361 aaggcgcatg gccagatctg gatttaaccg ctgacggcat ttgtaaaaac ggcgcagtga
     6421 acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg ggtaatacgg
     6481 cgctggcatg gctggaaaaa tacaacggtc cggaactgac acttacagcg tttgaaccac
     6541 cggccagctc ataatccagg tgggtagttg ggtttcatcc tgccatgcgc aatcgacaat
     6601 gtgaaaaccc tgcgcatggt agaaatctat cgccggttga tttttttgat aaacctccag
     6661 catcagatgg ggatggcgct gctgcacata ctgcatcagc gccttaccaa taccgcgcct
     6721 gacggccttc ggtgcgacaa acattgctgc gaggaatcgg ccttccataa tgctgacaaa
     6781 accgagaagc ttgccgtctt catcccagac ccagttttgc gcgttggcaa gatacgcatc
     6841 gcgcaccagc ggaatgcagt cacgccagta actcgatttt ataaagggat gcccccaggt
     6901 tgtactttcc agccacagtt cgaggatcgc ggggagttct gaatgttgca cttcccgaat
     6961 catggtttat ttcccggatg gcaacagcag ccaaccacat gatcattcac cagcccacat
     7021 gcctgcataa aggagtaaca gattgtggta ccgacaaact taaaaccacg tttttttagt
     7081 gccttagata gggcgtcgga ggcgggtgta gatgtgggga tttcgctcaa cgttgtggct
     7141 tgtgtcacct gtggttgatg attgacaaac gaccagacaa agtcggcaaa cggttcgccg
     7201 ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat tttcccacga
     7261 tggcggataa tcccggcgtc ctgtaccagt ctttcgatat cctcttcctg cattgcggca
     7321 actttcaccg gatcgaactg atgaaagcat gcgcgatagt tttcgcgttt tttgaggacg
     7381 gtgatccacg ataacccagc ctgctgccct tcaaggcaga tcatttcgaa cagtttttta
     7441 ctgtcagttt caggcacgcc ccattcatta tcatggtagg caatataaag cgggtcctga
     7501 ctcacccagc cgcaacgttc catactttcc ctcgctatgt acccgatcgt aaaaatttca
     7561 gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct actatgcccg
     7621 atatccttca tacaatgaca ataatattgc cgaattcggc tcttccctgg agtcgtgtta
     7681 atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgcttgcgag cgcggtaatc
     7741 agtgcctttt ttctcaacac agcttacgcc tggcaacaag aatatatcgt tgatacgcaa
     7801 cccggacatt ccacagagcg ttacacctgg gatagcgatc atcaacctga ttacaacgat
     7861 attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt caatctggcg
     7921 gaagaaaccc ctgtggatgt gaccagcagt atgagtatgg gctggaattt tcctttatat
     7981 gagcaggtta caaccggccc ggtcgcggca ttacattacg atggcacaac tacctcgatg
     8041 tataacgagt ttggcgacag tactaccacg ctggccgatc cgttatggca tgccagcgtg
     8101 agtaccttag gctggcgtgt tgactcccgg cttggcgatc ttcgaccctg ggcgcaaatc
     8161 agctataacc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct gagccggatg
     8221 acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga tatgttgctc
     8281 aatcaaaata ttgctgccta tgctgcgcta tctcaggcag aaaataccac taacaatagc
     8341 gactatctgt atacgatggg ggttagtgcc agattttaac gttatagata caattgaaac
     8401 ccataaataa caactaatgt ggcgatagtg gggactaact tactaaataa taaatttggc
     8461 gaataattgt cgagtcattc attcctgaac taatgcattt cattccgttc tgatggcatt
     8521 tcatgccgtt tttccccagg cataaagcgc acttcgttat ggttgtcagc agagattttt
     8581 cctttttatt actgcaggaa tactgccatg acaccttcaa attatcagcg tactcgctgg
     8641 ctgacactca tcggtactat cattacccag tttgctctgg ggtcggttta tacctggagt
     8701 ctgtttaacg gtgcgctttc tgccaagctg ggggctcctg taagccaggt tgctttctct
     8761 ttcggcttgt taagtctggg gctggcaatt tcgtcttctg ttgcgggcaa attgcaggaa
     8821 cgttttggtg ttaaacgcgt cactatggct tccggcattt tgctgggatt aggtttcttc
     8881 ctgacagcac attccaacaa cctgatgatg ctgtggttaa gcgccggtgt gttggttggt
     8941 ctggcggatg gtgcgggtta cctgttgacg ctctctaact gcgtgaagtg gtttccggag
     9001 cgtaagggac ttatctctgc ttttgctatc ggttcttatg gtctgggcag cctgggtttt
     9061 aaatttatcg acacgcacct gctcgaaacg gtgggtctgg aaaaaacctt tgttatttgg
     9121 ggcgcgattg tactggtgat gattgtcttt ggcgcaacgt taatgaaaga tgcgccgaag
     9181 caggaagtga aaaccagcaa tggtgtggtg gagaaggact acaccctggc agagtcgatg
     9241 cgtaaaccgc agtactggat gttagcggtt atgttcctga ctgcgtgcat gagtggtctg
     9301 tatgtgattg gtgtagcgaa agatatcgct caaagtctgg cgcatcttga tgcaatttcc
     9361 gcagccaatg ctgtgacggt tatttccatc gccaaccttt ctggtcgtct ggtgctgggc
     9421 attctgtctg acaaaatcgc ccgtatccgt gttatcacca ttggtcaggt gattgcgctg
     9481 gtgggtatgg cggccctgct gtttgcacca ttgaatgcag tgacgttctt tgcagcgatt
     9541 gcctgtgtgg catttaactt tggtggcacg attactgtgt tcccgtcgct ggttagtgag
     9601 ttcttcggcc tcaataacct ggcgaaaaac tacggcgtca tttacctcgg tttcggtatc
     9661 ggcagcattt gtggttcgat tatcgcttca ctgtttggcg gattctatgt gactttctac
     9721 gtgattttcg ccctgctgat tctgtcactg gcgctttcta cgaccattcg ccagccagag
     9781 cagaaaatgt tgcgtgaggc gcatggctcc ctttaatcat ctgccgcgag ctactgggct
     9841 tcagcttcag tgtgcatcaa acgcatcaac gaaatgtata aacaataacg cgtaaaattt
     9901 gggccagtta gctggccctt tttactgctt tttgtaacgc taaagtctct tttcaaactt
     9961 gcatttttgt aaatttgtgc ttcgtgcaca ctctttcccc acactttttc cctttgctgt
    10021 ggtctactta ttcgcgcgtg tagattttac ttatctgact acctccgcac ttttcccctg
    10081 cctggcctga aaagccacta agcagggtgt tatcacctgt ttgtccaggg tttgtttgca
    10141 tgagatacat caaatcgatt acacagcaga agctgagctt cttgcttgca atctatattg
    10201 gcctttttat gaatggcgcg gttttttacc gccgcttcgg cagctatgcg cacgatttta
    10261 ccgtctggaa aggtatttct gctgttgttg aactggccgc caccgtactg gtgaccttct
    10321 ttttactgcg tcttctttcg ctgtttggcc gccgcagctg gcgtattctg gcatcgctgg
    10381 tggtgctctt ttccgcaggt gccagctatt acatgacctt ccttaatgta gtcattggtt
    10441 atggcatcat cgcatccgtc atgaccaccg atatcgacct gtcaaaagaa gttgttggtc
    10501 tgaactttat tctctggtta atcgccgtaa gcgcgttgcc tcttatcctt atctggaata
    10561 accgctgtcg ttacaccttg ctccgacaac tgcgaacccc agggcaacgt attcgcagcc
    10621 tggcggtcgt cgtactggca ggtattatgg tttgggcacc gattcgtttg ctggatatcc
    10681 agcagaagaa agtggagagg gcgacaggtg ttgatttgcc gagttatggc ggcgtcgtcg
    10741 cgaactctta tctgccatca aactggcttt cggcattagg tctttatgcc tgggcgcggg
    10801 tcgatgaatc ttccgataac aattctctcc ttaatcctgc gaagaagttt acgtaccagg
    10861 ctccacaaaa cgttgatgat acatatgtcg ttttcattat tggtgaaacc acgcgctggg
    10921 atcatatggg cattttcggc tatgaacgca ataccacacc gaagctggct caggagaaaa
    10981 atctggcggc gttccgtggt tactcctgtg atactgcaac caaactctca ctgcgttgca
    11041 tgtttgtacg tcagggtgga gcagaagata atccgcagcg cacattaaaa gaacagaaca
    11101 ttttcgcggt attgaagcag ttaggattca gttctgatct ctacgctatg caaagcgaaa
    11161 tgtggttcta cagcaatacg atggcggaca acattgctta tcgtgagcag attggtgcgg
    11221 agccacgtaa ccgtggcaaa ccggtagatg atatgttgct ggtagacgaa atgcagcaat
    11281 cgctggggcg caacccggat ggtaagcatc tgatcattct gcataccaaa ggctcacact
    11341 ttaactacac ccagcgttac ccgcgcagct ttgcgcagtg gaagccggaa tgtattggtg
    11401 ttgatagcgg ctgtaccaaa gcgcagatga tcaactcata cgacaactcg gtgacctatg
    11461 tggatcactt tatttccagc gtgattgatc aggttcgcga taagaaagcg attgtgttct
    11521 atgcagctga ccacggtgag tcaattaatg aacgcgagca cctgcacggc acgccgcgtg
    11581 aactggcacc gccggaacag ttccgtgtgc cgatgatggt ctggatgtcg gataaatatc
    11641 tggaaaatcc ggtcaatgcg caggcatttg cgcagctgaa aaaagaagcc gacatgaaag
    11701 tgccacgccg tcatgtagag ctgtacgaca ccatcatggg ttgtcttggc tatacttcac
    11761 cggatggtgg aattaacgaa aacaacaact ggtgccacat cccgcaggca agagaggcgg
    11821 cggctaacta agaggcctgc tgactttctc gccgatcaaa aggcattttg ctattaaggg
    11881 attgacgagg gcgtatctgc gcagtaagat gcgccccgca ttcggtgatt ggcgcagcct
    11941 ggtagcgcac ttcgttcggg acgaaggggt cggaggttcg aatcctctat caccgaccaa
    12001 attcgaaaag cctgctcaac gagcaggctt ttttgcatct gtaactcatg aggaagagaa
    12061 cccccggggg cggggaggtt cgactcgacg taggcctgat aagacgcgcc agcgtcgcat
    12121 caggcaaatg taccggccca ttgtcggatg cggcgcgacc gccttatccg atctacacac
    12181 cacacatttc cctgccaaat ttgcgcccca tatctcacta tccattaacg gatttgtgac
    12241 agaaaccatt gtcttttttt atatttgctt aaatcttttt ttgcgttact tatggctggc
    12301 gctggcattt tccgctgtgc gttttagcgt tcatggtatt aatcgctcag atatccacct
    12361 ttacctcaga tttttttcat taaatgcata aaacttaatc accaaatgtt gctaaatatt
    12421 aagctgattg ttctggttgt gttggcgtag cacaaatttc tctgctgcaa atagctgttt
    12481 gaagtttttt taatctttgt ttgtggattc tcaccgttgg tgtaaatccc ggttggctgt
    12541 attgacgttt tcacattctg ttgacagatt gtaggtcacg aggggcattt tatggaggat
    12601 ccgcactgtt acactgatgt taattagtac ggcatcccca cctcataacg ttgacccgac
    12661 cgggcaaaaa acaaaaaagg tcaggcagcg acaacccact gcaaagggtt aaaacaacaa
    12721 acatcacaat tggagcagaa taatgcgtat ttccttgaaa aagccaggga tgctgaagct
    12781 tggtctcagc ctggtggcta tgaccgtcgc agcaagtgtt caggctaaaa ctctggttta
    12841 ttgctcagaa ggatctccgg aagggtttaa cccgcagctg tttacctccg gcaccaccta
    12901 tgacgcctct tccgtaccgc tttataaccg tctggttgaa tttaaaatcg gcaccactga
    12961 agtcatcccg ggcctcgctg aaaagtggga agtcagcgaa gacggtaaaa cctatacctt
    13021 ccatctgcgt aaaggtgtga agtggcacga caataaagaa ttcaaaccga cgcgcgaact
    13081 gaatgccgat gacgtagtgt tctcgtttga tcgtcagaaa aacgcgcaaa acccgtacca
    13141 taaagtctct ggcggcagct acgaatactt cgaaggcatg ggcttgccag agctgatcag
    13201 cgaagtgaaa aaggtggacg ataacaccgt tcagtttgtg ctgactcgcc cggaagcgcc
    13261 gttcctcgct gacctggcaa tggacttcgc ctctattctg tcaaaagaat atgctgacgc
    13321 gatgatgaaa gccggtacac cggaaaaact ggacctcaac ccaatcggta ccggcccgtt
    13381 ccagttacag cagtaccaga aagattcccg tattcgctat aaagcgtttg atggctactg
    13441 gggcaccaaa ccgaagatcg atacgctggt cttctctatt actcctgacg cttccgtgcg
    13501 ttacgcgaaa ttgcagaaga acgaatgcca ggtgatgccg tacccgaacc cggcagatat
    13561 cgcccgcatg aagcaggata aatccatcaa cctgatggaa atgccggggc tgaatgtcgg
    13621 ttatctctcg tataacgtgc agaaaaaacc actggatgac gtgaaagttc gccaggctct
    13681 gacctacgcg gtgaacaaag acgccatcat caaagccgtt tatcagggcg cgggcgtatc
    13741 agcgaaaaac ctgatcccgc caaccatgtg gggctataac gacgacgttc aggactacac
    13801 ttacgatcct gaaaaagcga aagccttgct gaaagaagcg ggtctggaaa aaggtttctc
    13861 catcgacctg tgggcaatgc cggtacaacg tccgtataac ccgaatgctc gccgcatggc
    13921 ggagatgatt caggcagact gggcgaaagt gggcgtgcag gccaaaatcg tcacctacga
    13981 atggggtgag tacctcaagc gtgcgaaaga tggcgagcat cagacggtaa tgatgggctg
    14041 gactggcgat aacggggatc cggataactt cttcgccacc ctgttcagct gcgccgcctc
    14101 tgaacaaggc tccaactact caaaatggtg ctacaaaccg tttgaagatc tgattcaacc
    14161 ggcgcgtgct accgacgacc acaataaacg tgttgaactg tacaaacaag cacaggtcgt
    14221 gatgcacgat caggctccgg cactgatcat cgctcactcc accgtgtttg aaccggtacg
    14281 caaagaagtt aaaggctatg tggttgatcc attaggcaaa catcacttcg aaaatgtctc
    14341 tatcgaataa ttaaaagcca tacaagattg atggcaaacg caaaatgcct gatgcgctcc
    14401 gcttatcagg cctacgagag ttctgcaatg tattgagttt gcacgatttt gtaggtcgga
    14461 taaggcgttc acgccgcatc cggcatgaac aaagcgcact ttgtcaacaa tctgcatacc
    14521 cggtggcgct gtgcctttaa gtgcagcgcc atccggcagc aatacttcat tccctgtccg
    14581 acaagggcgg gaattgattt gtgagcaata cagacacgca gttccaggct gcgggtcatt
    14641 atagagaatc cgggttatgt tgcagtttat tctccgacgt ttgggactcg tcatccccac
    14701 gtttatcggt attacccttc tcacatttgc ctttgtccac atgatcccgg gcgatccggt
    14761 gatgatcatg gcgggcgaac gtgggatctc cccagagcgt cacgcgcaat tgctggctga
    14821 actcggctta gataaaccga tgtggcagca gtatctccat tacatttggg gcgtaatgca
    14881 cggtgattta ggcatttcga tgaaaagccg aattccggta tgggaagagt tcgtgccgcg
    14941 ctttcaggcc acgctggaac ttggcgtctg cgcgatgatt tttgcgaccg cagtcgggat
    15001 tccggttggt gtgctggctg cggttaaacg tggttccatt ttcgatcaca cagcggttgg
    15061 cctggcgctg accggctact cgatgccaat tttctggtgg ggcatgatgc tgatcatgct
    15121 ggtttcggtg cactggaacc tgacgcctgt ctccggtcgt gtgagcgaca tggtgttcct
    15181 cgatgattcc aatccgttaa ccggttttat gctgatcgac accgccatct ggggtgaaga
    15241 cggtaacttt atcgatgccg tcgcccatat gatcctgccc gccattgtgc tgggtactat
    15301 tccgctggcg gtcattgtgc gtatgacgcg ctcctcgatg ctggaagtgc tgggcgaaga
    15361 ttatatccgc accgcgcgtg ccaaaggtct tacccggatg cgggtgatta tcgtccatgc
    15421 gctgcgtaac gcgatgctgc cggtggtgac cgttatcggc ctgcaggtgg gaacattgct
    15481 ggcgggggcg attctgactg aaaccatctt ctcgtggccc ggtctggggc gctggttgat
    15541 tgacgcactg caacgccgcg attatccggt agtgcagggc ggcgtattgc tggtggcgac
    15601 gatgattatc ctcgtcaacc tgctggtcga tctgctgtac ggcgtggtga acccgcgtat
    15661 tcgtcataag aagtaagggg acatcatgtc acaggttact gaaaataaag tgattagcgc
    15721 accggtgccg atgaccccgt tacaggagtt ctggcactat tttaaacgca acaaaggcgc
    15781 ggttgtcggg ctggtttacg tcgtcatcgt gctgtttatc gcgatctttg ccaactggat
    15841 tgcaccctat aacccagcgg aacagttccg cgatgcactg ctcgccccgc cagcctggca
    15901 ggaaggcggc agcatggcgc acttgctggg cactgatgac gtggggcgtg atgtgctgtc
    15961 tcggctgatg tacggcgcgc gcttgtcgct gctggttggc tgtctggtag tggtgttatc
    16021 gctgattatg ggcgttattc tcggcctgat cgctggttac tttggcggtc tggtcgataa
    16081 catcatcatg cgcgtggtcg atatcatgct ggcgctgcca agtctactgc tggcactggt
    16141 gctggtggcg attttcggcc cgtcgattgg taacgccgcg ctggcgctaa ccttcgttgc
    16201 gctgccgcat tatgtgcgct taacccgcgc cgccgtgctg gtggaagtta accgcgatta
    16261 cgtcaccgca tctcgcgtgg cgggtgccgg ggcaatgcgc cagatgttta ttaacatctt
    16321 cccgaactgc cttgcgccgc tgattgttca ggcgtcgctc ggtttctcta acgccattct
    16381 cgatatggct gctcttggtt tcctcggcat gggcgcgcag ccgccaacgc cggagtgggg
    16441 caccatgctc tctgacgtgt tgcagttcgc gcaaagcgcc tggtgggtcg tgaccttccc
    16501 gggtctggcg atcctgctga cggtgctggc atttaacctg atgggtgacg gtctgcgtga
    16561 cgcgctcgat cccaaactga agcagtaaga ggttcgagat ggcgttatta aatgtagata
    16621 aattatcggt gcatttcggc gacgaaagcg caccgttccg cgccgtagac cgcatcagct
    16681 acagcgtaaa acagggcgaa gtggtcggga ttgtggggga gtccggctct ggtaaatcgg
    16741 tcagttcgct ggcaattatg gggctgattg attatccggg ccgcgtaatg gcggaaaagc
    16801 tggagtttaa cggtcaggat ttgcagcgta tctcagaaaa agagcgccgc aacctggtgg
    16861 gtgccgaagt ggcaatgatc ttccaggacc cgatgaccag ccttaacccg tgctacaccg
    16921 tgggtttcca gattatggaa gcgattaagg tgcatcaagg cggcaacaaa agtacccgcc
    16981 gtcagcgagc gattgacctg ctgaatctgg tcggtattcc cgatccggca tcgcgtctgg
    17041 atgtttaccc gcatcagctt tccggcggca tgagccagcg cgtgatgatc gccatggcga
    17101 ttgcctgtcg gccaaaactg ctgattgccg atgaaccgac cactgcgctg gacgtgacca
    17161 tccaggcgca aatcattgag ctgctgctgg agctacagca aaaagaaaat atggcgctgg
    17221 tgctgatcac ccatgacctg gcgctggtgg cggaagcggc acataaaatc atcgtgatgt
    17281 atgcaggcca ggtagtagaa acgggcgatg cgcacgctat cttccatgcg ccgcgtcacc
    17341 cgtatactca ggcattgctg cgtgcgctgc cggaatttgc tcaggacaaa gaacgtctgg
    17401 cgtcgttgcc aggtgtcgtt cccggcaagt acgaccgccc gaacggctgc ctgcttaacc
    17461 cgcgctgccc ctacgccact gacaaatgcc gtgctgaaga accggcgctg aatatgctcg
    17521 ctgacgggcg tcagtccaaa tgccattacc cacttgatga tgccgggagg ccgacactat
    17581 gagtacgcat gaggccacct cgcaacaacc gctgttgcag gctatcgacc tgaaaaaaca
    17641 ttatccggtg aagaaaggca tgttcgcgcc ggaacgtctg gtgaaggcgc tggatggcgt
    17701 ttcgtttaac cttgaacgtg gcaaaacgct ggcagtagta ggtgaatctg gctgcggtaa
    17761 atcgaccctc ggtcggttgc tgacgatgat tgaaacgccc accggcggcg aactgtatta
    17821 ccaggggcag gatctgctca agcacgatcc gcaggcgcag aagctacgtc ggcagaaaat
    17881 ccagattgtc ttccagaacc cttacggttc gctgaatccg cgtaaaaaag ttgggcagat
    17941 tctcgaagag ccgctgctga tcaacaccag cttaagcaaa gaacagcgtc gggaaaaagc
    18001 cctgtcgatg atggcgaaag tcggcctgaa aaccgagcat tacgaccgct atccgcatat
    18061 gttctccggc ggtcagcgtc agcgtatcgc cattgcccga ggtctgatgc tcgacccgga
    18121 tgtggtgatt gccgatgagc cggtttccgc gctggacgtc tcagtgcgtg cgcaggtgct
    18181 gaatctgatg atggatttgc agcaggaact ggggctatct tatgtcttta tctcccacga
    18241 tctgtcggtg gtggagcaca ttgctgatga agtgatggtg atgtacctgg gccgctgcgt
    18301 ggagaagggg acgaaggacc aaattttcaa taacccgcgc cacccgtaca ctcaggcgct
    18361 gctctccgcg acaccacgcc tgaacccgga cgatcgtcgc gaacgcatca aacttatcgg
    18421 tgaactgcca agcccgctga atccaccgcc gggctgcgcc ttcaacgccc gctgtcgtcg
    18481 acgcttcggc ccctgcaccc agttgcagcc gcagctaaaa gactacggcg gtcaactggt
    18541 agcctgtttt gctgttgatc aggatgaaaa tccgcagaag ccagctgtat aactctcccg
    18601 atgagatcag ctttcatgct gatctcatgt caaatatgac ctttttatgc tttatctatc
    18661 agttatgtgt cttttttgac ctttttcttt gctaatgggt tatatatgag ctaaattata
    18721 accattaatg aaaaatagtt catttgtgac acttaacgaa tttatctgac cataaatgat
    18781 cttgtttttg tcttttatca atcgaaaggt tctttaatga tctgctcagg accacgaaat
    18841 ctggctcaag agttgaaagc gatcggcgac cagctccagg agttgcagaa gaaacttatt
    18901 caggggccga atattagtca accacgcccc ctcctgacta ttgagactcc ccgtcatttg
    18961 ggggaacaac tgaacgcacg gcgcaaagaa ttgggcattg atttatacac acttgaacta
    19021 caaacgggga tctcaacgtc cacgttaaag cgacttttta aggatcctga acaggttaaa
    19081 ttcggcagtg tgttcgcggt ggcgaacgtt cttggagtga aactatgcat cgacgagtga
    19141 aagtgttgct ctatggtcag gtagtgggtg aactctctca gaacgacagt ggttttttgt
    19201 tccagtacgc ccaggattac cacggaccgg caatctcaat cagcctgccc gtcgcacagc
    19261 gtcagtttcc cagtgagacg ttgcatcctt atttcgcctc actggcgcca gaaggatggc
    19321 tgcgtcaacg ctacagccag atacagcatc gtgacgaaaa cgatctgctg ggaatgctta
    19381 ttgataacgg caagaatctg ctgggggcaa tccagatttt accttgggag gaataatggc
    19441 gaattgtcgt attctgttaa cgccgttgaa tgaacgtgac gaacagcgcg gatattcgac
    19501 acaggggttg aagcgactga gcggcactgc aaaattgacc cccaggctgg ggtttacgcg
    19561 aactcagttt gtgcaggaac ttccgcgtca acaaaaaggg atgagtattt ccggttacca
    19621 gcccaaattg caattagtgc tggatgaagg agagttccgc gtcgtagatc atcagggaaa
    19681 ttttatcctt aagccctctc cagccgactt tcccggcttg gccgaaaatg aacacgcaac
    19741 aatgacattg atgtctcgtt tgggatttga cgtacctgtg catggcctgt tgagttttgc
    19801 accacaatca gaggaagagc ttgaatacgc atttgttatc cggcgctatg accgggatta
    19861 caaggggctg ccagtgcatc aggaacagct tgatggcgcg atgcagatta cagataaata
    19921 tggcaaaacg gggaatgata acgaacaata tgtcagctat gaaacgctgg cccgattttt
    19981 agttgcccat gttaacgaca acatcgcatt taaaatcgat ctctttcgtc gcattgttta
    20041 cgcctggttg ctgggtaata acgacatgca tttacgaaac tttggtttag tgtactccga
    20101 tggtttgacg ccaacgttag cgccggtgta tgactttgtt tctgtcgctc cctatccgga
    20161 atatttttac tcaaactatc tggcgctgcc gttattgacg cgagaagaag gcggtcggga
    20221 actggctcct ggatttcaca gtgattatgg cgagtatatc gggcaggact ttttgctgtt
    20281 gggtgaatca atggggttag cgcccagatt gctggaaaaa ctctttcagg atatacgtaa
    20341 agaaaatgcc attgttatgg aaacgtatga gcagtccttt atgactcagg atcatatcca
    20401 ggctgtgctg caatgctatc gacatcgtct ggggctgctg tgagcagccc ccgttacttt
    20461 tattcaatca gcttcaatag cggcgaaaag cacagcaaaa taccataaaa caggataagc
    20521 catgttttta gcccgcgtag ttttttcagt tgtgaaactt tagagatcag gaaaaacgga
    20581 atgaggcacg acacaatgcc atataacggg cttcccaact gaaagaacac cagtaccgat
    20641 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagtgcagat cgccagagtc
    20701 agcacgcgtg agttaatttt cctggtatca ataattcggc ttaacagatt gagaataatg
    20761 cctttaatgg cctcgtggaa accgagataa atgccaaaga atgcagtcag tacggcaaag
    20821 atattcagca ccgtagaggt gatatgaatg atatgcccag ggatcacctg cgcggccagt
    20881 gccagtgccg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    20941 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgacataact aattcggtgg
    21001 gtacgcagcg cgagccgggt tgccagtact ttatccgctt cccgtttacg ataggcaata
    21061 ttcattgggt taagtacctg aataaatact gcggaaaaga agcaaaatgg aatagtaagc
    21121 aggacatcgc ggaaaaagtc ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    21181 tgcgggatca tcgcaaaacc gaacacgaca ataatcccca ctttgaccac caccattggc
    21241 ccggaaatct taaacagtaa tcgttcacca ccagacgcaa tcgccaccag tacggcgaaa
    21301 atggcgactt tataaaatag agattgagaa agatcggcat cggttaaacc gaaggttttc
    21361 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    21421 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    21481 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgt
    21541 actacccagg tggcaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    21601 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    21661 aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct gatcgtgttt cggtagtgtg
    21721 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    21781 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    21841 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactactagt
    21901 ctggagtcag cccggatatg agctgcaact attgcaaata aagaaattaa gctggaaggc
    21961 atcacgcaat gaggaagatt gccgggtgct gacggcaaaa agcaatcgta aaatcagcgg
    22021 caagccggaa gggttccggt gaggcgcaat gttgcggggg ctttatccct ggtggcattg
    22081 gttgctggag agagaaaacc cccgcacgtt gcaggtatgc acctgacaac atcacggggg
    22141 ctaatcttga acctagacca ctcaagaata gccgcgaaac gttgtcatta caacacaggc
    22201 ggctatatga cgctcgcaga actgggcatg gccttctggc atgatttagc ggctccggtc
    22261 attgctggca ttcttgccag tatgatcgtg aactggctga ataagcggaa gtaacgtgtc
    22321 atgcgggcgt caggctgccg taatggcaat ttgcgcccgg atcaggccgc agggggaaac
    22381 tctgcggcct ttttcgttct tactgcgggt aaggcaccca gtcgccgccg ttcaggcgaa
    22441 cgtacggttt atcctggtat tgaataacta ctgcatttga gttctcagag accggtgctg
    22501 tttgtggtaa cccactggtg agttttttcc agtcaacatt gtcttcggtg aaaatcttgc
    22561 catcaagaac gcgaaccacc agatcggaga tagccaggaa gctgctcggt tggtcgatga
    22621 caatcggagc cccctgatgt ggtgccttca tgcctaagaa tttcacccca acggggacgt
    22681 ctgtgataga cgggctaggg atatcacgta gaccagatac ctgcattctg tcgcccttca
    22741 gcgcgccgcc gtgttccggc accacgacca ccatcacttt acgacccgat ttctccagtt
    22801 cagtaaagaa ggcgtccagt tcatcaaaga atttctgcgc ccgcgctttg taatccgctg
    22861 ttttgctgac gcccggataa tggttgccgt catgcagtgg aagcgtgttg tagaacgtgg
    22921 cactacggct gtttttatct ttttcggtaa cgtccagcca gcggttaagc acggcggtat
    22981 cgtcataaac cggcgaacca tcaaagccca gcaaaataac cggcagattt ttttgatcca
    23041 tcaattcgct ctgcatgccg ccattttcgc gaacttcttt caaaaaaccg ccgaactggc
    23101 cgttatgtcc catcatcagg tgctgggtaa agcccagttt cgacaggtta tcaaacagat
    23161 agcagtcgtt atttgccggt tgatacagat tagtgtgcga agtctgcccg cagctggcgc
    23221 gcagtaaacg gatcgccgcc gggccactgt aggaggtggc ggagttaaag ttcttgaact
    23281 caatatcgaa atgcgaccac agtggatgcg acatcaatcc ggcggcttca atatccgacc
    23341 aggaaagcga acagatatta atcaccagta gttcaaatgg ctgagcatca gcgggcagcg
    23401 aagacgggaa gatcgattta cgtttcgcct ccgcgttata gaaattattc agccaggcgt
    23461 taaggtttgc tgttgttggc ggtgcagttt gtgcgggtat atcacccact accggtgcgc
    23521 cacccgtcgc cgcaacggtt gccgctgcgt taccacccgt cgttgttaca gtggtcgtcg
    23581 gttgaccggc tggccacaag gagaaacttg gtcccgccag ggtaagtacg ttcagccata
    23641 gcagaatggc aaccacaaaa acggtaatgc gaatccattg tgacaggaat aaccaggcca
    23701 cgaataaaac aaaaatggcc ccaatcatct gccagttaat aaagcgtgtg acaagatcga
    23761 ttaaataatc ggtactgaac cccgccacct gcgaaccctg gctcattatg ctttccgggc
    23821 caggcaacca ggtgtcatgc cagaacaaag caaagccgat cggcagggca atccagtggc
    23881 gcaagcgatg caggctgtag cgcggaatgg gcatcagcag aaacgcggca aacaccagat
    23941 tgaggagcgg atggaagtta agatatcccg cccacaacag gccgaactta accagaaaat
    24001 agaagttcca gccggaaagg ccgcgccagt attgccagag ggaagaaggc atggcggtat
    24061 tttgcgtaaa ttgagtcatt ttttggttgc cctggctttt tccgtgcggc gtaacgtccc
    24121 ggccggttta acataacgag gtttagcaaa gaacaaacgt aaggtgtcgc gaatgcgtcg
    24181 caaagagtgc cgcgccagat agcccagcgg gaaaaatatc agtgcgcaaa cgacaataat
    24241 ttcaatgata tcgctgatgg tcatcatgat gagcgctcca cagcatcatc taacagtcgc
    24301 attggttctg gtattcgtcg ccagtggcga ccatcgtgct cggcattgat gaccggttta
    24361 gaactttgcg ctaaaggcag cggcatgccc cattgttctg gggcaagcag gcgcatctgc
    24421 accagctcgg cactgatttg atcatcttca aaccagacca ttcggtttga gaaaatgtcg
    24481 ccagtaggca atgggaaaat atgattcaac gcggtatcca gatcgttaat ccgacagaat
    24541 gagagaaaca gcaccagccg attaccgcca atggtcatga tatcgccggt acggttaggg
    24601 cgacatagcg tcagggcttg ctcaacgcgg atacccggta ccggacgtag ggcaaccaga
    24661 acgcctttac cgtgggcagg taatagaggg ttattcatca tgttgttgac ggcattacag
    24721 aacacatccc acttctggaa accacgcagt ttgagcggct gggtcattga cagcaaggta
    24781 gtgatatctt ccggcacata gcgactaaac ttctgccctt gcacgctttc gatcatcgtc
    24841 agacagcggg agagtggcgc attccacggg ataaccatat ttgcaccgca ggccaataat
    24901 aaacgttcat cggtggcgcg caggctagcg gtattttccc gtacgaggat tttcatcgca
    24961 ctaccgcgct ggcgacgcag agtatgaatg ctgcgggcca gtggctcgat ttgcgcattt
    25021 tgctgtaaag aaaagaccac cgtcgccgcc tgagcggtac gggcttcatt gaacaggact
    25081 tcgttattgt tgaacagttg ccagtgttcc gatagcggcg gcgcaccttc cagtacagca
    25141 acattactca gaatgcgttt ttcgtcgctg cgtggttgga tttccgcctc ctggtgttga
    25201 actaatgtcc agcaaccatt ttgttgctga acgctaagct gctgacgggc gctgacccct
    25261 ttttcgttgc accagaaagc aatatccagc aaatgttggt cgccctgaaa acgcaaactg
    25321 gcaagaccaa aaagtgaacg gtattcctca agtaacaatg aaaattgttt atcgttatta
    25381 tttccggaat taattaccaa aagcgaacaa tggtttagcc ggctccattt attcatttta
    25441 tccaaccatg agcgaagccg ctcggcagga atgttttgcc atgcgttatt tgcgcaaaca
    25501 agaataaaaa ggtaattatg gggatcaata gaacactgca aatcacgggc caaatagtat
    25561 agaccttttt catgatttag cattgaaaat aatgttattt ttcccggccc ttgagaatca
    25621 tctaattgaa agattttcgc tggatcgctg tccatgctaa tgaccgcgac gtgtgcggtt
    25681 tcagcctggg atgcaattgt ttgattcgcc agactgatag catcttcatg gcgatcgacg
    25741 ttaaaccacc agacgccgcc tgctggcata tgtcgcagct catcccataa tgatgagata
    25801 ccgatagaga atacagggtc cacaatgtcc ctcatgtcaa cattttgtct gattatcagt
    25861 ttactagcga aagctaagaa ataaacctac cattgaaatt aaaacttatc agatttagca
    25921 tgtgaatcaa tttagcaggt cacaagacat gtgattttag tcttttcgtt tttaactttt
    25981 tcggttgcgt aacggaaagt caaaaagtga gcacattccc gtctcgccgt aaacaataaa
    26041 ccgacgaaat gctcacagtt agaattgtct catgaacggt acggttattt catagggatc
    26101 aagaaaaatg aataacaatg aaccagatac tctgcctgat cccgcgatag gctatatctt
    26161 ccagaatgat attttggcgt taaagcaggc attttcactg cctgatattg attatgccga
    26221 tatttcccaa cgcgaacagt tggccgcggc attaaaacgc tggccgttgc tggcagagtt
    26281 tgcgcaacaa aagtagggga ttggtgaatg gccgtact
//
LOCUS       DAZFJL010000063        26192 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFJL010000063 DAZFJL010000000
VERSION     DAZFJL010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.063"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..144
                     /gene="tuf"
                     /locus_tag="ACHJ8C_002492"
     CDS             <1..144
                     /gene="tuf"
                     /locus_tag="ACHJ8C_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO5552335.1"
                     /translation="VEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAK
                     VLG"
     gene            327..521
                     /gene="bfd"
                     /locus_tag="ACHJ8C_002493"
     CDS             327..521
                     /gene="bfd"
                     /locus_tag="ACHJ8C_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO5552336.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            594..1070
                     /gene="bfr"
                     /locus_tag="ACHJ8C_002494"
     CDS             594..1070
                     /gene="bfr"
                     /locus_tag="ACHJ8C_002494"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO5552337.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1067..1534)
                     /locus_tag="ACHJ8C_002495"
     CDS             complement(1067..1534)
                     /locus_tag="ACHJ8C_002495"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO5552338.1"
                     /translation="MDATLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCYPDGLADALWGAIIGYSTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTLL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1913..2224
                     /gene="rpsJ"
                     /locus_tag="ACHJ8C_002496"
     CDS             1913..2224
                     /gene="rpsJ"
                     /locus_tag="ACHJ8C_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO5552339.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2257..2886
                     /gene="rplC"
                     /locus_tag="ACHJ8C_002497"
     CDS             2257..2886
                     /gene="rplC"
                     /locus_tag="ACHJ8C_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO5552340.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2897..3502
                     /gene="rplD"
                     /locus_tag="ACHJ8C_002498"
     CDS             2897..3502
                     /gene="rplD"
                     /locus_tag="ACHJ8C_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO5552341.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3499..3801
                     /gene="rplW"
                     /locus_tag="ACHJ8C_002499"
     CDS             3499..3801
                     /gene="rplW"
                     /locus_tag="ACHJ8C_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO5552342.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3819..4640
                     /gene="rplB"
                     /locus_tag="ACHJ8C_002500"
     CDS             3819..4640
                     /gene="rplB"
                     /locus_tag="ACHJ8C_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO5552343.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4657..4935
                     /gene="rpsS"
                     /locus_tag="ACHJ8C_002501"
     CDS             4657..4935
                     /gene="rpsS"
                     /locus_tag="ACHJ8C_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO5552344.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4950..5282
                     /gene="rplV"
                     /locus_tag="ACHJ8C_002502"
     CDS             4950..5282
                     /gene="rplV"
                     /locus_tag="ACHJ8C_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO5552345.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5300..6001
                     /gene="rpsC"
                     /locus_tag="ACHJ8C_002503"
     CDS             5300..6001
                     /gene="rpsC"
                     /locus_tag="ACHJ8C_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO5552346.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6014..6424
                     /gene="rplP"
                     /locus_tag="ACHJ8C_002504"
     CDS             6014..6424
                     /gene="rplP"
                     /locus_tag="ACHJ8C_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO5552347.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6424..6615
                     /gene="rpmC"
                     /locus_tag="ACHJ8C_002505"
     CDS             6424..6615
                     /gene="rpmC"
                     /locus_tag="ACHJ8C_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO5552348.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6615..6869
                     /gene="rpsQ"
                     /locus_tag="ACHJ8C_002506"
     CDS             6615..6869
                     /gene="rpsQ"
                     /locus_tag="ACHJ8C_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO5552349.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7034..7405
                     /gene="rplN"
                     /locus_tag="ACHJ8C_002507"
     CDS             7034..7405
                     /gene="rplN"
                     /locus_tag="ACHJ8C_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO5552350.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7416..7730
                     /gene="rplX"
                     /locus_tag="ACHJ8C_002508"
     CDS             7416..7730
                     /gene="rplX"
                     /locus_tag="ACHJ8C_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO5552351.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNATTGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7745..8284
                     /gene="rplE"
                     /locus_tag="ACHJ8C_002509"
     CDS             7745..8284
                     /gene="rplE"
                     /locus_tag="ACHJ8C_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO5552352.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8299..8604
                     /gene="rpsN"
                     /locus_tag="ACHJ8C_002510"
     CDS             8299..8604
                     /gene="rpsN"
                     /locus_tag="ACHJ8C_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO5552353.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8638..9030
                     /gene="rpsH"
                     /locus_tag="ACHJ8C_002511"
     CDS             8638..9030
                     /gene="rpsH"
                     /locus_tag="ACHJ8C_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO5552354.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9043..9576
                     /gene="rplF"
                     /locus_tag="ACHJ8C_002512"
     CDS             9043..9576
                     /gene="rplF"
                     /locus_tag="ACHJ8C_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO5552355.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9586..9939
                     /gene="rplR"
                     /locus_tag="ACHJ8C_002513"
     CDS             9586..9939
                     /gene="rplR"
                     /locus_tag="ACHJ8C_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO5552356.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9954..10457
                     /gene="rpsE"
                     /locus_tag="ACHJ8C_002514"
     CDS             9954..10457
                     /gene="rpsE"
                     /locus_tag="ACHJ8C_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO5552357.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10461..10640
                     /gene="rpmD"
                     /locus_tag="ACHJ8C_002515"
     CDS             10461..10640
                     /gene="rpmD"
                     /locus_tag="ACHJ8C_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO5552358.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10644..11078
                     /gene="rplO"
                     /locus_tag="ACHJ8C_002516"
     CDS             10644..11078
                     /gene="rplO"
                     /locus_tag="ACHJ8C_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO5552359.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11086..12417
                     /gene="secY"
                     /locus_tag="ACHJ8C_002517"
     CDS             11086..12417
                     /gene="secY"
                     /locus_tag="ACHJ8C_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO5552360.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12449..12565
                     /gene="rpmJ"
                     /locus_tag="ACHJ8C_002518"
     CDS             12449..12565
                     /gene="rpmJ"
                     /locus_tag="ACHJ8C_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO5552361.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12712..13068
                     /gene="rpsM"
                     /locus_tag="ACHJ8C_002519"
     CDS             12712..13068
                     /gene="rpsM"
                     /locus_tag="ACHJ8C_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO5552362.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13085..13474
                     /gene="rpsK"
                     /locus_tag="ACHJ8C_002520"
     CDS             13085..13474
                     /gene="rpsK"
                     /locus_tag="ACHJ8C_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO5552363.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13508..14128
                     /gene="rpsD"
                     /locus_tag="ACHJ8C_002521"
     CDS             13508..14128
                     /gene="rpsD"
                     /locus_tag="ACHJ8C_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO5552364.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14154..15143
                     /gene="rpoA"
                     /locus_tag="ACHJ8C_002522"
     CDS             14154..15143
                     /gene="rpoA"
                     /locus_tag="ACHJ8C_002522"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO5552365.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15184..15567
                     /gene="rplQ"
                     /locus_tag="ACHJ8C_002523"
     CDS             15184..15567
                     /gene="rplQ"
                     /locus_tag="ACHJ8C_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO5552366.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15674..16042
                     /gene="yhdN"
                     /locus_tag="ACHJ8C_002524"
     CDS             15674..16042
                     /gene="yhdN"
                     /locus_tag="ACHJ8C_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO5552367.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLVGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELENRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16053..16478
                     /gene="zntR"
                     /locus_tag="ACHJ8C_002525"
     CDS             16053..16478
                     /gene="zntR"
                     /locus_tag="ACHJ8C_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO5552368.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMGHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16534..16752
                     /gene="arfA"
                     /locus_tag="ACHJ8C_002526"
     CDS             16534..16752
                     /gene="arfA"
                     /locus_tag="ACHJ8C_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO5552369.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTVLLLSGVC"
     gene            complement(16749..17159)
                     /gene="mscL"
                     /locus_tag="ACHJ8C_002527"
     CDS             complement(16749..17159)
                     /gene="mscL"
                     /locus_tag="ACHJ8C_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO5552370.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLREAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17289..18665)
                     /gene="trkA"
                     /locus_tag="ACHJ8C_002528"
     CDS             complement(17289..18665)
                     /gene="trkA"
                     /locus_tag="ACHJ8C_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO5552371.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18687..19976)
                     /gene="rsmB"
                     /locus_tag="ACHJ8C_002529"
     CDS             complement(18687..19976)
                     /gene="rsmB"
                     /locus_tag="ACHJ8C_002529"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO5552372.1"
                     /translation="MKKQRNLRSMAAQAIEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGKQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20028..20975)
                     /gene="fmt"
                     /locus_tag="ACHJ8C_002530"
     CDS             complement(20028..20975)
                     /gene="fmt"
                     /locus_tag="ACHJ8C_002530"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO5552373.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNIVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEDKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLAI
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTTTKAAPGTILEANKQGIQVA
                     TGDGILNLLSMQPAGKKAMSVQDLLNSRREWFVPGNRLA"
     gene            complement(20990..21499)
                     /gene="def"
                     /locus_tag="ACHJ8C_002531"
     CDS             complement(20990..21499)
                     /gene="def"
                     /locus_tag="ACHJ8C_002531"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO5552374.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21629..22753
                     /gene="dprA"
                     /locus_tag="ACHJ8C_002532"
     CDS             21629..22753
                     /gene="dprA"
                     /locus_tag="ACHJ8C_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO5552375.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKATLQVN
                     GVSIAVLGNGLNTIHPRSHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22725..23198
                     /gene="smg"
                     /locus_tag="ACHJ8C_002533"
     CDS             22725..23198
                     /gene="smg"
                     /locus_tag="ACHJ8C_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO5552376.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLSLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23227..23769
                     /gene="yrdD"
                     /locus_tag="ACHJ8C_002534"
     CDS             23227..23769
                     /gene="yrdD"
                     /locus_tag="ACHJ8C_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO5552377.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTSHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23774..24346
                     /gene="tsaC"
                     /locus_tag="ACHJ8C_002535"
     CDS             23774..24346
                     /gene="tsaC"
                     /locus_tag="ACHJ8C_002535"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO5552378.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCKAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24351..25169
                     /gene="aroE"
                     /locus_tag="ACHJ8C_002536"
     CDS             24351..25169
                     /gene="aroE"
                     /locus_tag="ACHJ8C_002536"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO5552379.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVFRAEELAK
                     LFAHTGSIQALGMDKLEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            25166..25423
                     /gene="yrdB"
                     /locus_tag="ACHJ8C_002537"
     CDS             25166..25423
                     /gene="yrdB"
                     /locus_tag="ACHJ8C_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO5552380.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMKLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25399..25953)
                     /gene="yrdA"
                     /locus_tag="ACHJ8C_002538"
     CDS             complement(25399..25953)
                     /gene="yrdA"
                     /locus_tag="ACHJ8C_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO5552381.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTHP"
BASE COUNT         6734 a         6184 c         6780 g         6494 t
ORIGIN      
        1 gtagagatgg taatgccggg cgacaacatc aaaatggttg ttaccctgat ccacccgatc
       61 gcgatggacg atggtctgcg tttcgcaatc cgtgaaggcg gccgtaccgt tggcgcgggc
      121 gttgttgcta aagttctggg ctaattacac gttaattagt tttgaattga aaagggcgct
      181 tcggcgccct ttttgctgcc cgtaatttga gctcgaaata tttaatcgta atttcactcg
      241 ctgaactata gcgcaaataa aattgtgcta ttgtaatcat aattattctc atttacactt
      301 tgcgcagaat ttgaacggga gcaattatgt acgtttgttt gtgtaatggt gtaagtgaca
      361 aaaaaattcg ccaggcagtc cgtcagtttc atccgcaatc tttccaacaa ttgcgtaagt
      421 ttattcctgt gggaaatcaa tgcggtaagt gcattcgcgc cgcgcgagaa gtgatgcagg
      481 acgagttgat gcagatgcca gaatttaaag agattgcctg aagcccacgc ttattttttg
      541 acatcccgct agccccatct acgctctaat agtggaagcg gagggactat aaaatgaaag
      601 gtgatgttaa gatcataaat tatctcaata aactattggg aaatgagctt gtcgcaataa
      661 atcagtattt tctccatgcc cggatgttta aaaactgggg cctgatgcgt ctcaatgacg
      721 tcgaatacca tgaatccatc gatgagatga aacacgctga taaatatatc gagcgtattt
      781 tgtttttaga aggaatcccc aacttacagg atttaggcaa actggggatt ggtgaagatg
      841 tcgaggagat gctgcaatcc gacctgcgat tagagctgga aggcgcgaag gatctacgtg
      901 aggctatcgc gtatgccgac agcgttcatg actatgttag ccgtgatatg atgatcgaga
      961 tccttgccga tgaagaaggt catatcgact ggctcgaaac tgagctggat ctcatcggta
     1021 aaatcggtct acaaaattac ctgcaatcac aaataaaagt taaagattaa cggccggcca
     1081 gcaaactatc ccagccaaca acgaaacccg cagccgccag aaatggccca aatggcagcg
     1141 ggttttttaa tgactctttt cctctcatga gcagaccaat aactacggct ccgcaggcga
     1201 atgaggcagc aaggaaaacc agtcgtggca ataaggtcca tgtatgccag gcaccaaggg
     1261 cggcgagaaa cttcacatca ccatagccta atccttcttt atgacgcagt atgcgatacc
     1321 cccagtaaat gacagcgaat gtgctgtaac caataatcgc cccccataac gcatcggcta
     1381 aaccgtcagg ataacaaacc tggtagaaca aaagacctga ccagagtaac gggcaggtaa
     1441 atctgtcagg taacaagcca tgctttgcat cccagaaaaa aagtagggca gataaacaag
     1501 cgtacaaaat cagaaaggga agggtagcgt ccataaaaca tcctctgtaa ttagagtatc
     1561 accatcgaat aactccgggt gctcagcaac agagcaatcg ctgatttgtt aacctggttc
     1621 cggtgaaaaa tgctatcttc acaaccttac aaatttcgtg gaatacagct cgcaatctgc
     1681 gcgttacgat taatttctac ccgatgcttg cgtcaggccc agatttgcgt ataatgcgcg
     1741 ggcttgtcgt agttgacagc aggttcaatc tgaacctcag ttagccgaat ttggctacct
     1801 aacaatgctc ccaatcgggg agctacgtaa gaacggttac actctcccat caatcgtaat
     1861 gggtctgagg agtaatcatt ttcgtttata aaataattgg agctctggtc tcatgcagaa
     1921 ccaaagaatc cgtatccgcc tgaaagcgtt tgatcatcgt ctgatcgatc aagcaaccgc
     1981 ggaaatcgtc gagactgcta agcgcactgg tgcgcaggtc cgtggtccga tcccgctgcc
     2041 gacacgcaaa gagcgcttca ctgttctgat ctccccgcac gtcaacaaag acgcgcgcga
     2101 tcagtacgaa atccgtactc acttgcgtct ggttgacatc gttgagccaa ccgagaaaac
     2161 cgttgatgct ctgatgcgtc tggatctggc tgccggtgta gacgtgcaga tcagcctggg
     2221 ttaatcaggt cattgagcga ttgagaggtt gaaacaatga ttggtttagt cggtaaaaaa
     2281 gtgggtatga cccgtatctt cacagaagac ggcgtttcta tcccagtaac cgtaatcgaa
     2341 gttgaagcaa accgcgttac tcaggttaaa gacctggcta acgatggcta ccgtgctatt
     2401 caggtgacca ccggtgctaa aaaagctaac cgtgtgacca agcctgaagc tggccacttc
     2461 gctaaagctg gcgtagaagc tggccgtggt ctgtgggaat tccgcctggc tgaaggcgaa
     2521 gagttcactg taggtcagag cattagcgtt gaactgtttg ctgacgttaa aaaagttgac
     2581 gtaactggca cctctaaagg taaaggtttc gcaggtaccg ttaagcgctg gaacttccgt
     2641 acccaggacg ctactcacgg taactccttg tctcaccgcg ttccgggttc tatcggtcag
     2701 aaccagactc cgggcaaagt gttcaaaggc aagaaaatgg caggtcagat gggtaacgaa
     2761 cgtgtaaccg ttcagagcct tgacgtagta cgcgttgacg ctgagcgcaa cctgctgctg
     2821 gttaaaggcg ctgtcccggg tgcaaccggt agcgacctga tcgttaaacc agctgtgaag
     2881 gcgtaaggag atagcaatgg aattagtatt gaaagacgcg cagagcgcgc tgactgtttc
     2941 cgaaactacc ttcggtcgtg atttcaacga agcgctggtt caccaggttg ttgttgctta
     3001 tgcagctggt gctcgtcagg gtactcgtgc tcagaagact cgtgctgaag taactggttc
     3061 cggtaaaaaa ccgtggcgcc agaaaggcac cggccgtgcg cgttctggtt ctatcaagag
     3121 cccgatctgg cgttctggtg gcgtgacctt tgctgctcgt ccgcaggacc acagtcaaaa
     3181 agttaacaag aagatgtacc gcggcgcgct gaaaagcatc ctgtccgaac tggtacgtca
     3241 ggatcgtctg atcgttgtcg agaagttctc tgtagaagcg ccgaaaacta agctgctggc
     3301 acagaaactg aaagacatgg ctctggaaga tgtgctgatc atcaccggtg agctggatga
     3361 gaacctgttc ctggctgcgc gcaacctgca caaggttgac gtacgcgatg caactggtat
     3421 cgacccggtt agcctgatcg ccttcgacaa agtcgtaatg actgctgatg ctgttaagca
     3481 agttgaggag atgctggcat gattcgtgaa gaacgtctgc tgaaggtgct gcgtgcaccg
     3541 cacgtttctg aaaaagcgtc tactgcgatg gaaaaatcta acaccatcgt actcaaagtt
     3601 gctaaagacg cgaccaaagc agaaatcaaa gctgctgtgc agaaactgtt tgaagtcgaa
     3661 gtcgaagtcg ttaacaccct ggtagttaaa gggaaagtta aacgtcacgg acagcgtatc
     3721 ggtcgtcgta gcgactggaa aaaagcttac gtcaccctga aagaaggcca gaatctggac
     3781 ttcgttggcg gcgctgagta agtcggagga gtaatacaat ggcagttgtt aaatgtaaac
     3841 cgacatctcc gggtcgtcgc cacgtagtta aagtggttaa ccctgagctg cacaagggca
     3901 aaccttttgc tccgttgctg gaaaaaaaca gcaaatccgg tggtcgtaac aacaatggcc
     3961 gtatcaccac tcgtcatatc ggtggtggcc acaagcaggc ttaccgtatt gttgacttca
     4021 aacgcaacaa agacggtatc ccggcagttg ttgaacgtct tgagtacgat ccgaaccgtt
     4081 ccgcgaacat cgcgctggtt ctgtacaaag acggtgaacg ccgttacatc ctggccccta
     4141 aaggcctgaa agctggcgac cagattcagt ctggcgttga tgctgcaatc aaaccaggta
     4201 acaccctgcc gatgcgcaac atcccggttg gttctactgt tcataacgta gaaatgaaac
     4261 caggtaaagg cggtcagctg gcacgttccg ctggtactta cgttcagatc gttgctcgtg
     4321 atggtgctta tgtcaccctg cgtctgcgtt ctggtgaaat gcgtaaagta gaagcagact
     4381 gccgtgcaac tctgggcgaa gttggcaatg ctgagcatat gctgcgcgtt ctgggtaaag
     4441 caggtgctgc acgctggcgt ggtgttcgtc cgaccgttcg cggtaccgcg atgaacccgg
     4501 tagaccaccc acatggtggt ggtgaaggtc gtaactttgg taagcacccg gtaactccgt
     4561 ggggcgttca gaccaaaggt aagaagaccc gcagcaacaa gcgtactgat aaattcatcg
     4621 tacgtcgccg tagcaaataa ttttagagga taagccatgc cacgttctct caagaaaggt
     4681 ccttttattg acctgcactt gctgaagaag gtagagaaag cggtggaaag cggagacaag
     4741 aagcccctgc gcacttggtc ccgtcgttca acgatctttc ctaacatgat cggtttgacc
     4801 atcgctgtcc ataatggtcg tcagcacgtt ccggtatttg taaccgacga aatggtcggt
     4861 cacaaactgg gtgaattcgc accgactcgt acttatcgcg gccacgctgc tgataaaaaa
     4921 gcgaagaaga aataaggtag gaggaagaga tggaaactat cgctaaacat cgccatgctc
     4981 gttcttctgc tcagaaggtt cgccttgttg ctgacctgat tcgcggtaag aaagtgtcgc
     5041 aggctctgga tattttgacc tacaccaaca agaaagcggc tgtactggtc aagaaagttc
     5101 tggaatctgc cattgctaac gctgaacaca acgatggcgc tgacattgac gatctgaaag
     5161 ttacgaaaat tttcgtagac gaaggcccga gcatgaagcg cattatgccg cgtgcaaaag
     5221 gtcgtgcaga tcgcatcctg aagcgcacca gccacatcac tgtggttgtg tccgatcgct
     5281 gagactctgg agactagcaa tgggtcagaa agtacatcct aatggtattc gcctgggtat
     5341 tgtaaaacca tggaactcta cctggtttgc gaacaccaaa gaattcgctg acaacctgga
     5401 cagcgatttt aaagtacgtc agtacctgac taaggaactg gctaaagcgt ccgtatctcg
     5461 tatcgttatc gagcgtccgg ctaagagcat ccgtgtaacc attcacactg ctcgcccggg
     5521 tatcgttatc ggtaaaaaag gtgaagacgt agaaaaactg cgtaaggtcg tagcggacat
     5581 cgctggcgtt cctgcacaga tcaacatcgc cgaagttcgt aagcctgaac tggacgcaaa
     5641 actggttgct gacagcatca cttctcagct ggaacgtcgc gttatgttcc gtcgtgctat
     5701 gaagcgtgct gtacagaacg caatgcgtct gggcgctaaa ggtattaaag ttgaagttag
     5761 cggccgtctg ggcggcgcgg aaatcgcacg taccgaatgg taccgcgaag gtcgcgtacc
     5821 gctgcacact ctgcgtgctg acatcgacta caacacctct gaagcgcaca ccacttacgg
     5881 tgtaatcggc gttaaagtgt ggatcttcaa aggcgagatc ctgggtggta tggctgctgt
     5941 tgaacaaccg gaaaaaccgg ctgctcagcc taaaaagcag cagcgtaaag gccgtaaata
     6001 aggagcgtcg ctgatgttac aaccaaagcg tacaaaattc cgtaaaatgc acaaaggccg
     6061 taaccgcggt ctggcgcagg gtacggatgt tagcttcggc agcttcggtc tgaaagctgt
     6121 tggccgtggt cgtctgactg cacgtcagat cgaagcagca cgtcgtgcta tgacccgtgc
     6181 agttaagcgt caaggtaaga tctggatccg tgtgttcccg gacaaaccga tcactgaaaa
     6241 gccgctggca gtgcgtatgg gtaaaggtaa aggtaacgtg gagtattggg ttgccttgat
     6301 tcagccgggt aaagtcctgt atgaaatgga cggtgttccg gaagagctgg cccgtgaagc
     6361 attcaagctg gcagcagcga aactgccgat taaaaccacc tttgtaacta agacggtgat
     6421 gtaatgaaag caaaagagct gcgtgagaag agcgttgaag agctgaacac cgagctgctg
     6481 aacctgctgc gtgagcagtt caacctgcgt atgcaggctg caagtggcca gctgcaacag
     6541 tctcacctgt tgaagcaagt gcgtcgcgat gtcgcacgcg ttaagacttt actgaacgag
     6601 aaggcgggtg cgtaatgacc gataaaatcc gtactctgca aggtcgcgtt gttagcgaca
     6661 aaatggagaa atccattgtt gttgctatcg aacgttttgt gaaacacccg atctacggta
     6721 aattcatcaa gcgtacgacc aaactgcacg tacatgacga gaacaacgaa tgcggtatcg
     6781 gtgacgtggt tgaaatccgc gaatgccgtc cgctgtccaa gactaaatcc tggacgctgg
     6841 ttcgcgttgt agagaaagcg gttctgtaat acagtacact ctctagatac gaataaacgg
     6901 ctcagaaatg agccgtttat tttttctacc catatccttg aagcggtgtt ataatgccgc
     6961 gccctcgata tggggatttt taacgacctg attttcgggt ctcagtagta gttgacatta
     7021 gcggagcact aaaatgatcc aagaacagac tatgctgaac gtcgccgaca actccggtgc
     7081 acgtcgcgta atgtgtatca aggttctggg tggctcgcac cgtcgctacg caggcgtagg
     7141 cgacatcatc aagatcacca tcaaagaagc aattccgcgt ggtaaggtca aaaaaggtga
     7201 tgtgctgaag gcggtagtgg tgcgcaccaa gaagggtgtt cgtcgcccgg acggttctgt
     7261 cattcgcttc gatggtaatg cttgtgttct tctgaacaac aacagcgagc agcctatcgg
     7321 tacgcgtatt tttgggccgg taactcgtga gcttcgtagt gagaagttca tgaaaattat
     7381 ctctctggca ccagaagtac tctaaggagc gaatcatggc agcgaaaatc cgtcgtgatg
     7441 acgaagttat cgtgttaacc ggtaaagata aaggtaaacg cggtaaagtt aagaatgtcc
     7501 tgtcttccgg caaggtcatt gttgaaggta tcaacctggt taagaaacat cagaagccgg
     7561 ttccggccct gaaccaaccg ggtggcatcg ttgaaaaaga agccgctatt caggtttcca
     7621 acgtagcaat cttcaatgcg acaaccggca aggctgaccg tgtaggcttt agattcgaag
     7681 acggtaaaaa agtccgtttc ttcaagtcta acagcgaaac tatcaagtaa tttggagtag
     7741 tacgatggcg aaactgcatg attactacaa agacgaagta gttaaaaaac tcatgactga
     7801 gtttaactac aattctgtca tgcaagtccc tcgggtcgag aagatcaccc tgaacatggg
     7861 tgttggtgaa gcgatcgctg acaaaaaact gctggataac gcagcagcag acttggcagc
     7921 aatctccggt caaaaaccgc tgatcaccaa agcacgcaaa tctgttgcag gcttcaaaat
     7981 ccgtcagggc tatccgatcg gctgtaaagt aactctgcgt ggcgaacgca tgtgggagtt
     8041 ctttgagcgc ctgatcacta ttgctgtacc tcgtatccgt gacttccgtg gcctgtccgc
     8101 taagtctttc gacggtcgtg gtaactacag catgggtgtc cgtgagcaga tcatcttccc
     8161 agaaatcgac tacgataaag tcgaccgcgt tcgtggtttg gatattacca ttaccactac
     8221 tgcgaaatct gacgaagaag gccgcgctct gctggctgcc tttgacttcc cgttccgcaa
     8281 gtaaggtagg gttactaaat ggctaagcaa tcaatgaaag cacgcgaagt aaaacgcgta
     8341 gctttagctg ataaatactt cgcgaaacgc gctgaactga aagcgatcat ctctgatgtg
     8401 aacgcttccg acgaagatcg ttggaacgct gttctcaagc tgcagactct gccgcgtgat
     8461 tccagcccgt ctcgtcagcg taaccgctgc cgtcaaacag gtcgtccgca tggtttcctg
     8521 cggaagttcg ggttgagccg tattaaggtc cgtgaagccg ctatgcgcgg tgaaatcccg
     8581 ggtctgaaaa aggctagctg gtaattgtca ccaattgaat cacgggagta aagacagatg
     8641 agcatgcaag atccgatcgc ggatatgctg acccgtatcc gtaacggtca ggccgcgaac
     8701 aaagctgcgg tcaccatgcc ttcctccaag ctgaaagtgg caatcgccaa cgtgctgaag
     8761 gaagaaggtt ttattgaaga ttttaaagtt gaaggcgaca ccaagcctga actggaactt
     8821 actctgaagt atttccaggg caaagctgtt gtagaaagca ttcagcgtgt cagccgccca
     8881 ggtctgcgca tctataaacg taaagatgag ctgccgaaag ttatggcggg tctgggtatc
     8941 gcagttgttt ctacctctaa aggtgttatg actgatcgtg cagcgcgcca ggctggtctt
     9001 ggtggcgaaa ttatctgcta cgtagcctaa tcggaggaaa aaatgtctcg tgttgctaaa
     9061 gcaccggtcg ttgttcctgc cggcgttgac gtaaaaatca acggtcaggt tattacgatc
     9121 aaaggtaaaa acggcgagct gactcgtact ctcaacgatg ctgttgaagt taaacatgca
     9181 gataataccc tgaccttcgg tccgcgtgat ggttacgcag acggttgggc acaggctggt
     9241 accgcgcgtg ccctgctgaa ctcaatggtt atcggtgtta ccgaaggctt cactaagaag
     9301 ctgcagctgg ttggtgtagg ttaccgtgca gcggttaaag gcaatgtgat taacctgtct
     9361 ctgggtttct ctcatcctgt tgaccatcag ctgcctgcgg gtatcactgc tgaatgtccg
     9421 actcagactg aaatcgtgct gaaaggcgct gataagcagg tgatcggcca ggttgcagcg
     9481 gatctgcgcg cctaccgtcg tcctgagcct tacaaaggca agggtgttcg ttacgccgac
     9541 gaagtcgtgc gtaccaaaga ggctaagaag aagtaaggta acactatgga taagaaatct
     9601 gctcgtatcc gtcgtgcgac ccgcgcacgc cgcaagctcc aggagctggg cgcaactcgc
     9661 ctggtggtac atcgtacccc gcgtcacatt tacgcacagg taattgcacc gaacggttct
     9721 gaagttctgg tagctgcttc tactgtagaa aaagctatcg ctgaacaact gaagtacacc
     9781 ggtaacaaag acgcggctgc agctgtgggt aaagctgtcg ctgaacgcgc tctggaaaaa
     9841 ggcatcaaag atgtatcctt tgaccgttcc gggttccaat atcatggtcg tgtccaggca
     9901 ctggcagatg ctgcccgtga agctggcctt cagttctaag gtagaggtgt aagatggctc
     9961 acatcgaaaa acaagctggc gaactgcagg aaaagctgat cgcggtaaac cgcgtatcta
    10021 aaaccgttaa aggtggtcgt attttctcct tcacagctct gactgtagtt ggcgatggta
    10081 acggtcgcgt tggttttggt tacggtaaag cgcgtgaagt tccagcagcg atccagaaag
    10141 cgatggaaaa agcccgtcgc aatatgatta acgtcgcgct gaataacggc actctgcaac
    10201 accctgttaa aggtgttcac acgggttctc gcgtattcat gcagccggct tccgaaggta
    10261 ccggtatcat cgccggtggt gcaatgcgcg ccgttctgga agtcgctggg gttcataacg
    10321 ttctggctaa agcatatggt tccaccaacc cgatcaacgt ggttcgtgca actattgatg
    10381 gcctggaaaa tatgaattct ccagaaatgg tcgctgccaa gcgtggtaaa tccgttgaag
    10441 aaattctggg gaaataaacc atggcaaaga ctattaaaat tactcaaacc cgcagtgcaa
    10501 tcggtcgtct gccgaaacac aaggcaacgc tgcttggcct gggtctgcgt cgtattggtc
    10561 acaccgtaga gcgcgaggat actcctgcta ttcgcggtat gatcaacgcg gtttccttca
    10621 tggttaaagt tgaggagtaa gagatgcgtt taaatactct gtctccggcc gaaggctcca
    10681 aaaaggcggg taaacgcctg ggtcgtggta tcggttctgg cctcggtaaa accggtggtc
    10741 gtggtcacaa aggtcagaag tctcgttctg gcggtggcgt acgtcgcggt ttcgagggtg
    10801 gtcagatgcc tctgtaccgt cgtctgccga aattcggctt cacttctcgt aaagcagcga
    10861 ttacagccga agttcgtctg tctgacctgg ctaaagtaga aggcggtgta gtagacctga
    10921 acacgctgaa agcggctaac attatcggta tccagatcga gttcgcgaaa gtgatcctgg
    10981 ctggcgaagt aacgactccg gtaactgttc gtggcctgcg tgttactaaa ggcgctcgtg
    11041 ctgctatcga agctgctggc ggtaaaatcg aggaataagt agcagatggc taaacaaccg
    11101 ggattagatt ttcaaagtgc caaaggtggc ttaggcgagc tgaaacgcag actgctgttt
    11161 gttatcggtg cgctgattgt gttccgtatt ggctctttta ttccgatccc tggtattgat
    11221 gccgctgtac ttgccaaact gcttgagcaa cagcgaggca ccatcattga aatgtttaac
    11281 atgttctctg gtggtgctct cagccgtgct tctatctttg ctctggggat catgccatat
    11341 atttcggcat cgatcattat ccagctgctg acggtggttc acccaacgtt ggcagaaatt
    11401 aagaaagaag gggagtctgg tcgtcgtaag atcagccagt acacccgcta cggtactctg
    11461 gtgctggcaa tattccagtc gatcggtatt gctaccggtc tgccgaatat gcctggcatg
    11521 caaggcctgg tcattaaccc gggctttgca ttctacttca ccgctgttgt aagtctggtc
    11581 acaggaacca tgttcctgat gtggttgggc gaacagatta ctgaacgagg tatcggcaac
    11641 ggtatttcaa tcattatctt cgccggtatt gtcgcgggac tcccgccagc cattgcccat
    11701 actatcgagc aagcgcgtca aggcgacctg cacttcctcg tgttgctgtt ggttgcagta
    11761 ttagtatttg cagtgacgtt ctttgttgta tttgttgagc gtggtcaacg ccgcattgtg
    11821 gtaaactacg cgaaacgtca gcaaggtcgt cgtgtctatg ctgcacagag cacacattta
    11881 ccgctgaaag tgaatatggc gggggtaatc ccggcaatct tcgcttccag tattattctg
    11941 ttcccggcga ccatcgcgtc atggttcggg ggcggtactg gttggaactg gctgacaaca
    12001 atttcgctgt atttgcagcc tgggcaaccg ctttatgtgt tactctatgc gtctgcaatc
    12061 atcttcttct gtttcttcta cacggcgttg gttttcaacc cgcgtgaaac agcagataac
    12121 ctgaagaagt ccggtgcatt tgtaccagga attcgtccgg gagagcaaac ggcgaagtat
    12181 atcgataaag taatgacccg cctgaccttg gttggtgcgc tgtacattac ctttatctgc
    12241 ctgatcccgg agttcatgcg tgatgcaatg aaagtaccgt tctacttcgg tgggacctca
    12301 ctgcttatcg ttgttgtcgt gattatggac tttatggctc aagtgcaaac tctgatgatg
    12361 tccagtcagt atgagtctgc attgaagaag gcgaacctga aaggctacgg ccgataattg
    12421 gtcgcccgag aagttacgga gagtaaaaat gaaagttcgt gcttccgtca agaaattatg
    12481 ccgtaactgc aaaatcgtta agcgtgatgg tgtcatccgt gtgatttgca gtgccgagcc
    12541 gaagcataaa cagcgccaag gctgattttt tcgcatattt ttcttgcaaa gttgggttga
    12601 gctggctaga ttagccagcc aatcttttgt atgtctgtgc gtttccattt gagtatcctg
    12661 aaaacgggct tttcagcatg gaacgtacat attaaatagt aggagtgcat agtggcccgt
    12721 atagcaggca ttaacattcc tgatcataag catgccgtaa tcgcattaac ttcgatttat
    12781 ggcgtcggca agacccgttc taaagccatc ctggctgcag cgggtatcgc tgaagatgtt
    12841 aagatcagtg agctgtctga aggacaaatc gacacgctgc gtgacgaagt tgccaaattt
    12901 gtcgttgaag gtgatctgcg ccgtgaaatc agcatgagca tcaagcgcct gatggatctt
    12961 ggttgctatc gcggtttgcg tcatcgtcgt ggtctcccgg ttcgcggtca gcgtaccaag
    13021 accaacgcac gtacccgtaa gggtccgcgc aaaccgatca agaaataatc ggggtgattg
    13081 aataatggca aaggcaccaa ttcgtgcacg taaacgtgta agaaaacaag tctctgacgg
    13141 cgtggctcat atccatgctt ctttcaacaa caccatcgtg actatcactg atcgtcaggg
    13201 taacgcgttg ggttgggcaa cagccggtgg ttccggtttc cgtggttctc gcaaatccac
    13261 tccgtttgca gctcaggttg cagcagagcg ttgcgctgac gccgtgaaag aatacggcat
    13321 caagaatctg gaagttatgg ttaaaggtcc gggtccaggc cgcgaatcta ctattcgtgc
    13381 tctgaacgcc gcaggtttcc gcatcactaa cattactgat gtgactccga tccctcataa
    13441 cggttgtcgt ccgccgaaaa aacgtcgcgt ataacgcttc gttttccagg tttgttggag
    13501 aaagaaaatg gcaagatatt tgggtcctaa gctcaagctg agccgtcgtg agggcaccga
    13561 cttattcctt aagtctggcg ttcgcgcgat cgataccaag tgtaaaattg aacaagctcc
    13621 tggccagcac ggtgcgcgta aaccgcgtct gtctgactat ggtgtgcagt tgcgtgaaaa
    13681 gcaaaaagtt cgccgtatct atggtgtgct ggagcgtcag ttccgtaact actacaaaga
    13741 agcagcacgt ctgaaaggca acaccggtga aaacctgttg gctctgctgg aaggtcgtct
    13801 ggacaacgtt gtataccgta tgggcttcgg tgccactcgt gcagaagcac gtcagctggt
    13861 tagccataaa gcaattatgg taaacggtcg tgttgttaac atcgcttctt atcaggttag
    13921 tccgaatgac gttgtaagca ttcgtgagaa agcgaagaag cagtctcgcg tgaaagccgc
    13981 tctggagctg gctgagcagc gtgaaaagcc aacctggctg gaagttgatg ctggcaagat
    14041 ggaaggtacg tttaagcgta agccggagcg ttctgatctg tctgcggaca ttaacgaaca
    14101 cctgatcgtc gagctttact ccaagtaaag cttagtacca aagagaggac acaatgcagg
    14161 gttctgtgac agagtttcta aaaccgcgcc tggttgatat cgagcaagtg agttcgacgc
    14221 acgccaaggt gacccttgag cctttagagc gtggctttgg ccatactctg ggtaacgcac
    14281 tgcgccgtat tctgctctca tcgatgccgg gttgcgcggt gaccgaggtt gagattgatg
    14341 gtgtactaca tgagtacagc accaaagaag gcgttcagga agatatcctg gaaatcctgc
    14401 tcaacctgaa agggctggcg gtgagagttc agggcaaaga tgaagttatt cttaccttga
    14461 ataaatctgg cattggccct gtgactgcag ccgatatcac ccacgacggt gatgtcgaaa
    14521 tcgtcaagcc gcagcacgtg atctgccacc tgaccgatga gaacgcgtct attagcatgc
    14581 gtatcaaagt tcagcgcggt cgtggttatg tgccggcttc tacccgaatt cattcggaag
    14641 aagatgagcg cccaatcggc cgtctgctgg tcgacgcatg ctacagccct gtggagcgta
    14701 ttgcctacaa tgttgaagca gcgcgtgtag aacagcgtac cgacctggac aagctggtca
    14761 tcgaaatgga aaccaacggc acaatcgatc ctgaagaggc gattcgtcgt gcggcaacca
    14821 ttctggctga acaactggaa gcttttgttg acttacgtga tgtacgtcag cctgaagtga
    14881 aagaagagaa accagagttc gatccgatcc tgctgcgccc tgttgacgat ctggaattga
    14941 ctgtccgctc tgctaactgc cttaaagcag aagctatcca ctatatcggt gatctggtac
    15001 agcgtaccga ggttgagctc cttaaaacgc ctaaccttgg taaaaaatct cttactgaga
    15061 ttaaagacgt gctggcttcc cgtggactgt ctctgggcat gcgcctggaa aactggccac
    15121 cggcaagcat cgctgacgag taaccagatc acaggttaag gttttactga gaaggataag
    15181 gtcatgcgcc atcgtaagag tggtcgtcaa ctgaaccgca acagcagcca tcgccaggct
    15241 atgttccgca atatggcagg ttcactggtt cgtcatgaaa tcatcaagac gactctgcct
    15301 aaagcgaaag agctgcgccg cgtagttgag ccgctgatta ctcttgccaa gactgatagc
    15361 gttgctaatc gtcgtctggc attcgcccgt actcgtgata acgagatcgt ggcaaaactg
    15421 tttaacgaac tgggcccgcg tttcgcgagc cgtgccggtg gttacactcg tattctgaag
    15481 tgtggcttcc gtgcaggcga caatgcgccg atggcttaca tcgagctggt tgatcgttca
    15541 gagaaagcag aagctgctgc agagtaatct gaagcaacgt aaaaaaaccc gccccggcgg
    15601 gtttttttat acccgtagca tccccactta tctacaatag ctgtactctt tttgttcatc
    15661 ccctggagta tttatgtggt tacttgacca gtgggcagag cgtcatatag cagaagcgca
    15721 agcgaaaggt gagtttgata acctggtagg tagcggcgaa ccattgatac tggatgatga
    15781 ttctcatgtg ccaccggaat tacgtgcggg gtatcgcttg ctgaagaatg ctggttgctt
    15841 accgccagaa cttgagaata ggagggaagc aatacagctt ctggatattc tcaaaggtat
    15901 ccgtcatgat gatccgcaat atcaagaggt tagtcgtcga ttgtcattac tggaattgaa
    15961 gctgcgacaa gctggattga gtaccgattt tttacgcggc gattatgctg acaagttgtt
    16021 gaacaaaatc aacgataact agtggagtat gtatgtatcg cattggtgag ctggcaaaaa
    16081 tggcggaagt aacacccgac acgattcgtt attacgaaaa gcagcagatg atggggcatg
    16141 aagtacgcac cgaaggtggg tttcgcctgt ataccgaaag cgatctccag cgattgaaat
    16201 ttatccgcca tgccagacaa ctaggtttca gtctggagtc gatccgcgag ttgctgtcga
    16261 tccgtatcga tccggaacac catacctgtc aggagtcaaa aggcattgtg caggaaagat
    16321 tgcaggaagt cgaagctcgt atagccgagt tgcagagtat gcagcgttcc ttgcaacgcc
    16381 ttaacgatgc ctgttgtggg accgcccata gcagtgttta ttgctcgatt cttgaagctc
    16441 ttgaacaagg ggcgagtggt gttaagagtg gctgttgatt ttttgcactg gcaggattac
    16501 actcgcgccg ttaaataacc aactggagtt tttatgagcc gatatcagca tactaaaggg
    16561 cagataaagg ataatgcgat agaagcatta ctacatgatc ccttattccg acagcgagta
    16621 gagaaaaata agaaagggaa aggcagttac atgcgaaaag gaaaacatgg caatcggggg
    16681 aactgggagg ccagtggcaa gaaagtaaat cactttttta ccactgtcct tctgctttca
    16741 ggcgtttgtt aagagcggtt attctgctct ttcagcaaat cacgaatttc tgtcagtaat
    16801 acttcttctt tagttggtgc aggtgcggct gctggttctt cttttttccg attcagtttg
    16861 ttgattagct taatcgccat aaagatggca aaggccacta tcagaaaatc aaagacgttt
    16921 tgaatgaaga caccgtaatg catcacaaca gcagggatat ccccttgggc ctcgcgtagc
    16981 gtgacagcaa actgtttaaa gtcgatcccg ccaattaata agcccagagg tggcatgatg
    17041 atatcggcaa ccagtgaaga tacaatcttc ccgaatgccg ctccgataat gacacccact
    17101 gccaaatcca ccacgttccc gcgcatcgca aattcgcgaa attctttaat aatgctcatg
    17161 ttattctccc tatgaagccg acaaccataa gtctaacaaa tgttaagcca ttttcctgcc
    17221 aggaatggat ttaattaatc cttaataatc aaagggaaat aataaggcgt cattagacgc
    17281 cttattaatt acaagaagaa aggacttggc tggaagagtc tttcgacgtc ggtaataaac
    17341 tttttatctg tgaggaacat aattacgtga tcgccttgct caatgcgcag attgtcattg
    17401 gcaatcatca catcgtttcc acgtaccacc gctccaataa tcgttcctgg cggtagcttg
    17461 atttcgtcaa tgactctgcc gacaacgcgt gaggtgcttt catcaccgtg agcaacggct
    17521 tcaatagctt ctgctacgcc gcggcgtaat gaggaaacac caacaatatc tgcttttcgc
    17581 acatggctaa gcaacgcaga aatagttgct tgttgtggtg aaatcgcaat atcgatcacg
    17641 ctcccctgaa ccagatccac ataagcgcga cgttggatca ataccatcac ctttttcgca
    17701 cccatacgtt tggcaagcat ggcggacatg atattggcct cgtcatcgtt ggtgacggca
    17761 ataaacagat caacttgatc gatatgttct tcggccagca gttcttgatc cgacgcatca
    17821 ccaaaaaaga cgatcgtatt ctgcaacttt tccgccagtt cggcagcgcg ctgctgatta
    17881 cgttcgatga gtttaacgct gtaatctttt tccagacgac gcgccagccc tgcaccgata
    17941 ttaccgccac caaccagcat gatccgctta tacggttttt ccagtcgctg taattcactc
    18001 atcaccgcac ggatatgctg tgaagcggca ataaagaaca cttcatcacc agcttcaaca
    18061 atggtcgaac cttgcggacg aatggggcga tcgtggcgga aaattgctgc cacacgagta
    18121 tcgatatgtg gcatatgttc gcgcatggtt gaaagtgcat taccaatcag cgggccgcca
    18181 taataggctt taaccacagc caggctgact ttaccctcag cgaagttcac cacctgcaat
    18241 gcgccgggat actcaatcag tcggtaaata ttatcgataa ccaactgctc tggtgcgatc
    18301 agatgatcaa tcggtacagc atctgaatga aatagcttat cggcatcgcg cacgtagtct
    18361 ggtgagcgga tacgagcgat gcgattaggg gtgttgaaaa gtgagtaagc tacctggcag
    18421 gcaaccatat tggtttcatc tgaactggtt acagcaacca gcatatcggc gtcgtcggca
    18481 cctgcctccc gcaatacgcg tggatgagag ccatgcccct gcacgacccg caggtcaaat
    18541 ttatcctgta aggtccgcag acgctcaccg ttggtatcga caacagtaat atcgttgttc
    18601 tcgccaacca ggttttccgc cagtgtgccg ccaacctggc cggcacccag aatgataatt
    18661 ttcatcagtc gcgacccgtt atctcatcac tttttgatta gcttagcgta aaagaagccg
    18721 tcgccctctt cggcaccagg cagattttgt ttacccggtt gctctggtgt tcctgtttcg
    18781 caaagttcgg catcagcggt acgttgcaaa aaggctttaa tctgcaggct attctcttcc
    18841 ggtaacaccg aacaggtggc atagaccagg gttccaccgg attttaaatg cggccaaatg
    18901 gcgtcgagaa tttcagactg caattgcgcg agttctggga tatcgcgatc gcgacgtaac
    18961 catttaatat ccggatggcg acgaatcacc cccgttgctg aacaaggcgc atctaataaa
    19021 atgcgatcaa actgttgctt gccacaccat tgggaagggt agcggccatc accttgtttc
    19081 acggtcgcct tcataccaag gcgttttaaa ttgtcgtaaa cgcgagagag gcgctgttcg
    19141 tcaatatcaa ccgcaacaac ctgcgcttct ggtgccacct caaggatatg cgttgtttta
    19201 ccgccggggg cagcacaaag atccagaatg tgttcaccgt tttgtggcgc aagccaggtc
    19261 atgcaacctt gtgctgatgc atcctgaacg gtaacccatc cgtcttcaaa accaggtagc
    19321 gcatgaacag gtgcaggtgt ttccagacgt acagcatcag ggtaatccgc atgcgggaaa
    19381 cctttcattc ctgcttcatc cagcaatgca agccagctgt cgcgggaatg atgcgtacga
    19441 ttaacgcgca gccacattgg cggacgctgg ttattggctt ctacgatgga ttgccactgc
    19501 tctggatacg ctttttgcag acgtttcagc aaccaggaag gatgcagata acgtgcatca
    19561 ctggcattaa actcggctaa taactcttct tgctgacgtt ggaactggcg taatacgccg
    19621 ttaatcaacc ctttaagttg cggacgctta attgcgacag caccttcaac cgtttcagcc
    19681 agcgcagcat gaggtggaat gcgggtataa agcagttgat acaaaccaac cataatcagg
    19741 taatgcacag tgcgctgttt gccggtcatc ggacgggcca ttaacttatt aatcagccag
    19801 tctaactgcg aaagcgtacg cagtacgcca aagcataact cctggagaag agctttatct
    19861 ttatcggaaa ctttttgctg gagcggtggc agaatgttgc tcagtgattg cccttgctca
    19921 acgacttgtt caatggcctg ggccgccatg ctacgtaaat tacgttgttt tttcataagt
    19981 ataaatataa agatgcccgg taagaccggg cttagaagag tggactatca ggccagacgg
    20041 ttgcccggta caaaccattc ccgacgagaa ttcaggaggt cttgcacgct catcgctttc
    20101 ttacctgcag gttgcattga gagcagattc aggatgccat cacctgtcgc tacctgaatg
    20161 ccttgtttat tggcttcaag gattgttcct ggcgcagcct ttgttgtcgt atcgatgaca
    20221 gatgctttcc agactttaac cggctgccct tcaatttcca gccagctcat tggccatgga
    20281 ttgaaagcgc gaatgcaacg ttcaagctgt gctgccgaaa gtgaccagtc aatacgcgct
    20341 tcttctttac tcaacttttc ggcgtaagtg acaagagttt cgtcctgaac ttctggtttc
    20401 gccgtgccgt ctgccagttg tttcaacgta gtaataagcc cttgtgggcc aagctctgcc
    20461 agtttgtcgt acagcgtacc actggtatct tctgcagtaa tcgggcagga gagcttatag
    20521 agcatgtcac cggtgtctaa accaacatcc atttgcataa tggtcacacc agtttctgca
    20581 tcacccgccc atagtgagcg ttggattggt gcagcaccgc gccagcgtgg cagcagtgaa
    20641 ccatgaacgt tgatacagcc aagacgcggt atcgccaaca ctgctttagg cagaattaaa
    20701 ccataggcga cgacgaccat aacatcagcc tgcagatcgg cgaccagttg ctgattttct
    20761 tgtggacgca gggaaacagg ttgaaaaacg ggcagacctt tgtcctcagc cagaacttta
    20821 accgggctgg gcatcagttt tttaccgcgt cctgccggtc ggtctggctg ggtaaacacg
    20881 ccaacgatgt tatgaccaga agacaacagc gcgtcgagat gacgcgctgc aaagtcaggt
    20941 gtacccgcaa aaataatacg tagtgattct gacacgttaa ttcttatcct taagcccggg
    21001 ctttcagacg atccagtttt tcaactttct gacgaatacg ttgttgtttc agcggtgaca
    21061 gataatccat aaacagtttg ccgaccaggt gatccatctc atgctgaata cagatggcta
    21121 acagaccgtc tgcttccagt tcaaatggtt taccgtcgcg gtccagggcg cgaattttaa
    21181 ctttctctgc gcgtggcact aaagcacgtt gttcagggat cgacaggcaa ccttcttcaa
    21241 tgcctgtttc gccgcttttt tctaaaagct ccggattgat taacaccagc cgttcgtcac
    21301 ggttttccga aacatcaatt acaatgatac gttgatggat atcaacctgg gttgccgcca
    21361 ggccaatacc ttcttctgcg tacatcgtct cgaacatatc atcgacgata cgctgaattt
    21421 ctgcattcac ttcttctacc ggtttagcaa ctttgcgaag ccgctcgtcc ggaatatgta
    21481 acacttgcaa aactgacata aatctccaga gatgtgttca ggagttagaa agattatttc
    21541 ttctattcta gacaaatccc cctctgattg acagcatcac tgaccaatcg caaagattgc
    21601 taaggctgct tatggcaggg agatagggat ggtcgataca gaaatttggc tgcgtttaat
    21661 gagcatcagc agcttgtatg gcgatgatat ggtccgtata gctcactggc tggcaaaaca
    21721 gtcgcatatt gatgcggttg tattgcagca aacagggctt acattgcggc aggcacaacg
    21781 ctttctgtca tttccgcgaa agagtatcga aagctcactt tgttggttgg agcaacccaa
    21841 ccatcattta atccctgcgg acagcgaatt ttatcctcct caacttctgg cgacgacaga
    21901 ttaccccggc gcactgtttg ttgaaggaga actgcacgcg ttgcattcat ttcagcttgc
    21961 cgtagtgggg agtcgggcgc attcatggta tggcgagcga tggggacgat tattttgcga
    22021 aactcttgcg acgcgtggag tgacaattac gagtggactg gcgcgtggaa tcgatggtgt
    22081 ggcgcataaa gcgaccttac aggtaaatgg cgttagcatt gctgtattgg ggaatggact
    22141 taataccatt catccccgca gccatgctcc actggctgcc agtctacttg agcaaggtgg
    22201 tgctctcgtc tcggaatttc ccctcgatgt tccacccctt gcttacaatt tcccacgaag
    22261 aaatcgcatt atcagtggtc taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag
    22321 tggttcgctg gtgacagcac gttgtgcgct tgagcagggg cgtgaagttt ttgctttgcc
    22381 aggtccaata gggaatccgg gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat
    22441 tcttgtgacg gaaccggaag aaattctgga aaacttgcaa tttggattgc actggttgcc
    22501 agacgcccct gaaaattcat tttattcacc agatcaggaa gacgtggcat tgccatttcc
    22561 tgagctcctg gctaacgtag gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc
    22621 cggccaacct gtgccagagg tagttactca actactcgaa ctggagttag caggatggat
    22681 cgcagctgta cccggcggct atgtccgatt gaggagggca tgccatgttc gacgtactaa
    22741 tgtatttgtt tgaaacctat attcacacag aagctgagtt gcgtgtggat caagacaaac
    22801 ttgaacagga tcttaccgac gcaggatttg atcgagaaga tatctacaat gccctgcttt
    22861 ggctggaaaa gcttgcggat tatcaggaag gactggcaga accgatgcaa ctggcctctg
    22921 atccactctc catgcgtatt tatacccctg aagagtgtga aagactggat gccagctgtc
    22981 gtggtttttt gcttttcctt gagcaaattc aggtgcttag ccttgagacc cgtgaaatgg
    23041 tgatagagcg agtgctcgcg ctggataccg cagagttcga cctggaagac ctgaaatggg
    23101 taatcctgat ggtgttgttc aatattccgg gctgcgaaaa tgcgtaccag caaatggaag
    23161 aattactctt tgaagtgaat gaaggtatgc tgcattaatc atttctttaa ttcagcataa
    23221 gtagttatgg cgaaatcagc actgttcacg gtgcgtaata atgagtcctg cccaaagtgc
    23281 ggggctgaac tagttattcg atccgggaaa cacggtccgt ttcttggatg ctcacagtat
    23341 ccggcgtgtg actacgtccg tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt
    23401 ctggaggggc aggtttgccc tgcatgtggc gcaaatctgg tattacgcca gggacgcttt
    23461 ggtatgttta ttggttgcag taactaccct gaatgcgaac ataccgaact tatcgataaa
    23521 ccggacgaaa cagcaattac atgcccccaa tgccggacga gccatctggt ccagcgccgc
    23581 tcccgttatg gcaaaacgtt tcactcctgt gatcgctacc cggagtgtca atttgccatt
    23641 aacttcaaac ccatagctgg agaatgccct gagtgtcatt atccgctact catcgaaaag
    23701 aaaaccgcgc agggtgtaaa acacttttgt gccagtaaac aatgtggaaa gccggtttcg
    23761 gcggaataat aacgtgaata ataacctgca aggagacgct atcgcagctg cgatagatgt
    23821 cctcaatgaa gaacgtgtca tcgcctatcc aacggaagcc gttttcggtg tcgggtgcga
    23881 tcctgatagc gaaacagcag tgatgcgact gttggagtta aaacagcgtc cggttgataa
    23941 ggggctgatt ctaatcgccg caaattacga gcagcttaaa ccctatattg atgacaccat
    24001 gctgactgac gcgcagcgtg aaactatatt ttctcgctgg ccaggtcctg tcacctttgt
    24061 ttttcccgcg cctgcgacaa caccgcgctg gttgacgggc cgctttgatt cgcttgctgt
    24121 acgagtcacc gaccatccgt tggtggttgc tttgtgcaag gcttatggta aaccgctggt
    24181 ttctaccagt gccaacttga gtggattgcc accttgtcga acagtagacg aagttcgcgc
    24241 acaatttggc gcggcgttcc cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc
    24301 agaaatccgc gatgccctga cgggtgaact gtttcgacag gggtaacata atggaaacct
    24361 atgctgtttt tggtaatccg atagcccaca gcaaatcgcc attcattcat cagcaatttg
    24421 ctcagcaact gaatattgaa catccctatg ggcgcgtgtt ggcacccatc aatgatttca
    24481 tcaacacact gaacgctttc tttagtgctg gtggtaaagg tgcgaatgtg acggtacctt
    24541 ttaaagaaga ggcttttgcc agagcggatg agcttactga acgggcagcg ttggctggtg
    24601 ctgttaatac cctcaagcgg ttagaagatg gacgcctgct gggtgacaat accgatggtg
    24661 taggcttgtt aagcgatctg gaacgtctgt cttttatccg tcctggttta cgtattctgc
    24721 tcatcggcgc tggtggagca tctcgcggcg tactactgcc acttctttcc ctggactgtg
    24781 cggtgacaat cactaatcgg acggtattcc gcgcggaaga gttggctaaa ttgtttgcgc
    24841 acactggcag tattcaggcg ttgggtatgg acaaactgga aggtcatgag tttgatctca
    24901 ttattaatgc aacatccagt ggcatcagtg gtgatattcc ggcgatcccg tcatcgctca
    24961 ttcatccagg catttattgc tatgacatgt tctatcagaa aggaaaaact ccttttctgg
    25021 catggtgtga gcagcgaggc tcaaagcgta atgctgatgg tttaggaatg ctggtggcac
    25081 aggcggctca tgccttcctt ctttggcatg gcgttctgcc tgatgtagaa ccggttatca
    25141 aactattgca acaggagttg tcagcgtgaa tcaggccatc cagtttccgg atagggaaga
    25201 gtgggacgag aataaaaaat gtgtatgttt tcccgctctc gtgaatggta tgaaactgac
    25261 atgtgcgatc tctggcgaga gtctggcgta tcgctttact ggagatacgc cagaacagtg
    25321 gttagcgagt tttcgtcagc atcgctggga cctggaagaa gaagcggaaa acttaattca
    25381 ggaacaaagt gaagatgatc aagggtgggt ctggttacct tgatccagat attcgtcctt
    25441 ccatttcacg tagttatttg cggaatagcg taacccagcc ttctcttcat cacttaacgg
    25501 gcggatctgt ttgacggggc taccgagata cagatatccg ctctccagcc gtttattttg
    25561 tgggaccaga ctacccgcac caatcatcac atcatcttct actattgcgc catcaagtaa
    25621 aattgagccc atcccaacca aaactcgatt gccaatggtg cagccgtgga gcatcacctt
    25681 gtgaccaaca gtgacatctt cgccaatggt taatgggttg ccatctgggt tgtacgagga
    25741 tttatgagtg acatgcaaca tactgccatc ctggatattg gtgcgtgctc cgatctgtac
    25801 ataatgtaca tctccacgaa tcacaacgag cggccagatc cccacatcat cagccagacg
    25861 aacgtcacca atcacgacgc tgctatcgtc gatcattacg cgctgaccga tttgtggaaa
    25921 aagatcgcgg tatgggcgta aaacatcaga catactcacc tcaacaataa atgatttcct
    25981 aatgactttg ggtgcattat tggccttgtg caagtctttt agtatgcaaa aaagcaccgt
    26041 tttgtgtgcg aatgcagcaa aaagggtgaa aaaacaacaa acagaaaaaa agatcaaaaa
    26101 agtacttgtg caaaaaattg ggatccctat aatgcgcctc cgttgagacg acaacgtgaa
    26161 acacttcaca agatggtcgt aacaacgaag ag
//
LOCUS       DAZFJL010000064        25758 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFJL010000064 DAZFJL010000000
VERSION     DAZFJL010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.064"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..372)
                     /gene="acpS"
                     /locus_tag="ACHJ8C_002539"
     CDS             complement(<1..372)
                     /gene="acpS"
                     /locus_tag="ACHJ8C_002539"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO5552382.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVII"
     gene            complement(372..1103)
                     /gene="pdxJ"
                     /locus_tag="ACHJ8C_002540"
     CDS             complement(372..1103)
                     /gene="pdxJ"
                     /locus_tag="ACHJ8C_002540"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO5552383.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVCILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLADAGIQVSLFIDADEDQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFATSLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1115..1843)
                     /gene="recO"
                     /locus_tag="ACHJ8C_002541"
     CDS             complement(1115..1843)
                     /gene="recO"
                     /locus_tag="ACHJ8C_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO5552384.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(1855..2760)
                     /gene="era"
                     /locus_tag="ACHJ8C_002542"
     CDS             complement(1855..2760)
                     /gene="era"
                     /locus_tag="ACHJ8C_002542"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO5552385.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(2757..3437)
                     /gene="rnc"
                     /locus_tag="ACHJ8C_002543"
     CDS             complement(2757..3437)
                     /gene="rnc"
                     /locus_tag="ACHJ8C_002543"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO5552386.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(3709..4683)
                     /gene="lepB"
                     /locus_tag="ACHJ8C_002544"
     CDS             complement(3709..4683)
                     /gene="lepB"
                     /locus_tag="ACHJ8C_002544"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO5552387.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(4699..6498)
                     /gene="lepA"
                     /locus_tag="ACHJ8C_002545"
     CDS             complement(4699..6498)
                     /gene="lepA"
                     /locus_tag="ACHJ8C_002545"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO5552388.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRGNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(6696..7175)
                     /gene="rseC"
                     /locus_tag="ACHJ8C_002546"
     CDS             complement(6696..7175)
                     /gene="rseC"
                     /locus_tag="ACHJ8C_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO5552389.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCSSRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7172..8128)
                     /gene="rseB"
                     /locus_tag="ACHJ8C_002547"
     CDS             complement(7172..8128)
                     /gene="rseB"
                     /locus_tag="ACHJ8C_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO5552390.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDIGSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGTAQ"
     gene            complement(8128..8778)
                     /gene="rseA"
                     /locus_tag="ACHJ8C_002548"
     CDS             complement(8128..8778)
                     /gene="rseA"
                     /locus_tag="ACHJ8C_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO5552391.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATTNN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(8811..9386)
                     /gene="rpoE"
                     /locus_tag="ACHJ8C_002549"
     CDS             complement(8811..9386)
                     /gene="rpoE"
                     /locus_tag="ACHJ8C_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO5552392.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9383..9538)
                     /gene="rseD"
                     /locus_tag="ACHJ8C_002550"
     CDS             complement(9383..9538)
                     /gene="rseD"
                     /locus_tag="ACHJ8C_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO5552393.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            9794..11416
                     /gene="nadB"
                     /locus_tag="ACHJ8C_002551"
     CDS             9794..11416
                     /gene="nadB"
                     /locus_tag="ACHJ8C_002551"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO5552394.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVGKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRIANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTKEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDISRRIPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSAGNHYINR"
     gene            complement(11401..12138)
                     /gene="trmN"
                     /locus_tag="ACHJ8C_002552"
     CDS             complement(11401..12138)
                     /gene="trmN"
                     /locus_tag="ACHJ8C_002552"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO5552395.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12270..13604
                     /gene="srmB"
                     /locus_tag="ACHJ8C_002553"
     CDS             12270..13604
                     /gene="srmB"
                     /locus_tag="ACHJ8C_002553"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO5552396.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(13637..14518)
                     /gene="yfiE"
                     /locus_tag="ACHJ8C_002554"
     CDS             complement(13637..14518)
                     /gene="yfiE"
                     /locus_tag="ACHJ8C_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5552397.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVISGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQPLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLHQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVVKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFLPG"
     gene            14621..15208
                     /gene="eamB"
                     /locus_tag="ACHJ8C_002555"
     CDS             14621..15208
                     /gene="eamB"
                     /locus_tag="ACHJ8C_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO5552398.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGITALSTFVLPQTQALSWIIGVSVLLAVIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(15263..15646)
                     /gene="grcA"
                     /locus_tag="ACHJ8C_002556"
     CDS             complement(15263..15646)
                     /gene="grcA"
                     /locus_tag="ACHJ8C_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO5552399.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            15951..16640
                     /gene="ung"
                     /locus_tag="ACHJ8C_002557"
     CDS             15951..16640
                     /gene="ung"
                     /locus_tag="ACHJ8C_002557"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO5552400.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHILKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGEKPI
                     DWMPVLPAESE"
     gene            complement(16688..17725)
                     /gene="yfiF"
                     /locus_tag="ACHJ8C_002558"
     CDS             complement(16688..17725)
                     /gene="yfiF"
                     /locus_tag="ACHJ8C_002558"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO5552401.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEESPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVLQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            17932..18351
                     /gene="trxC"
                     /locus_tag="ACHJ8C_002559"
     CDS             17932..18351
                     /gene="trxC"
                     /locus_tag="ACHJ8C_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO5552402.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            18420..19118
                     /gene="tapT"
                     /locus_tag="ACHJ8C_002560"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             18420..19118
                     /gene="tapT"
                     /locus_tag="ACHJ8C_002560"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO5552403.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPETLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSLDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTEAATGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            19150..21810
                     /gene="pat"
                     /locus_tag="ACHJ8C_002561"
     CDS             19150..21810
                     /gene="pat"
                     /locus_tag="ACHJ8C_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO5552404.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKSNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQALIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            21924..23279
                     /gene="pssA"
                     /locus_tag="ACHJ8C_002562"
     CDS             21924..23279
                     /gene="pssA"
                     /locus_tag="ACHJ8C_002562"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO5552405.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            23323..23646
                     /gene="yfiM"
                     /locus_tag="ACHJ8C_002563"
     CDS             23323..23646
                     /gene="yfiM"
                     /locus_tag="ACHJ8C_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO5552406.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YAQHQGMSRDRSATFGLMFSVGLGASKELWDSRPEGSGWSWKDFTWDVAGATTGYTVW
                     QLTRH"
     gene            complement(23643..24941)
                     /gene="kgtP"
                     /locus_tag="ACHJ8C_002564"
     CDS             complement(23643..24941)
                     /gene="kgtP"
                     /locus_tag="ACHJ8C_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO5552407.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(25267..25382)
                     /gene="rrf"
                     /locus_tag="ACHJ8C_002565"
     rRNA            complement(25267..25382)
                     /gene="rrf"
                     /locus_tag="ACHJ8C_002565"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(25483..>25758)
                     /locus_tag="ACHJ8C_002566"
     rRNA            complement(25483..>25758)
                     /locus_tag="ACHJ8C_002566"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         6204 a         6855 c         6417 g         6282 t
ORIGIN      
        1 aataattacc gtggcacaag catagtggcg ctcatcggca agcgttacat gcatatttac
       61 aacgccgagc ttttccgcca gttttaatgc ctcaccccat agccgtagcc gtggtttgcc
      121 gagctcatca ttgaatactt caaactgatt aaacgccaga ccattgcgga tccctgtgcc
      181 aaatgctttt gccgccgctt ctttcacagc aaaacgcttc gccagaaaac gcactggctg
      241 gtggtgcgtt ttccagattt cccattcgtt atcgcttaat acgcggcgtg ccaggcgctc
      301 accggatcgg gcgatcaccg cttctatgcg agcgatctct acaatatccg tacctaaacc
      361 taatattgcc attagccacg cgcttccagc atcagacgtt tcatttctgc caccgcatct
      421 ttcagtccgg tcatcactgc acgaccaata atggcatgac cgatattcag ttcatgcatc
      481 tcagggatgg cggcaatggc tttcacgttg tgataggtca gaccgtgtcc ggcgttaact
      541 ttcagaccga gactagtggc aaaggtcgcg gctttggcga tacgcgccaa ctcttgcgcc
      601 tgttcggcgt cagttttggc atcagcatag caaccggtgt ggatctcgat aaacggtgcg
      661 ccaacctctg ccgcagcttt gatctgatct tcatcggcgt cgataaacag agaaacctgg
      721 atcccggcat ctgccagacg tttgcaggca tcgcgcattt tttcacgctg ccctgcgacg
      781 tccaggccgc cttcggttgt tacttcctga cgcttttccg gtaccagaca gcaaaaatgt
      841 ggcttcgtct caacggcgat cgccagcatc tcttcggtca ccgccatctc cagattcatg
      901 cgggtatcca gcgtctggcg caggatgcac acatcgcggt cggtaatgtg gcggcggtct
      961 tcacgtaaat gcacggtaat gccgtccgct cccgcctgct cagcaataaa cgccgcctgc
     1021 accggatccg ggtaagcagt accgcgcgcg ttacgcagcg ttgcaatatg gtcaatgttg
     1081 acgcctaaca gtaattcagc catgacaatc ctcatcattc ataatgtgtt ttcaccgttc
     1141 gcttaggcat aaactgccgg aacagttccc tgctctttaa tggtttaccg ccaagatacg
     1201 gcttaagcgc catgcgggta aagcgtttcg cggcgcgcag tgtgtctgcg tcaggaaatt
     1261 cccgcgcgtt taacgctttt aactgccttc cggtgaacgt tttattgtcg ataacgacgc
     1321 ttgcgataaa ccctttttct tcgcgataac gatacgtcat ggtgtcatct accggctcgc
     1381 cgctacccgc acaatgggta aaattgacgc catagcccag atgcccgagc agtgccagtt
     1441 caaagcggcg caacgcgggt tctggcgtac cagtgacccc ggcaagagac tgaatgcagt
     1501 gcaagtaatc gaaaaagagt tcagagaagc gcgtctcgta ttccagtacg cgggagagaa
     1561 gttcgttgat gtacagaccg ctgtaaagcg tgataccgct taatggcagc gccagcgaga
     1621 cagcttcagc actgcgcagc gttttgactt cgccacgccc gccaaaacgt agcaagagag
     1681 gggtgaaagg ctgtaatgcc cctttcagag tagagcgttt agagcgtgcg cctttggcaa
     1741 ccagacgcac gcgacccgat tcctccgtga agacgtccag catcaggctg gtttcgctcc
     1801 acgggcgact atgcaggaca aatgcgcgct gccagccttc catcggagtt actcttaaag
     1861 atcgtcaaca taaccgagac tgcgcagtgc gcgttcgtcg tcggcccaac cggatttcac
     1921 ttttacccac agctcaaggt gaacaggcgc ttcgaacatt tcctgcatgt ctttacgcgc
     1981 ttcaatcccg atggttttga tcttggcccc tttgttgcca atgaccatct tcttctgccc
     2041 ttcacgctca acgagaatca aaccgttgat gtcataaccg ccgcgttcgt tagagacgaa
     2101 acgttcgatc tccacggtca cggagtacgg cagttcagcg ccgagaaaac gcatcagttt
     2161 ttcgcggata atttcagacg ccataaaacg ctgtgagcga tcggtgatgt aatcttccgg
     2221 gaagtgatgg gtcgcttcag gcagatgctt acgcacgatt gccgcgatag tgtcgacatt
     2281 cagcccggtt tcggcagaga tcggcacgat atcgaggaag ttcatctggc ttgccaggaa
     2341 ctgtaagtgt ggcagcagat cggctttttc ctgcacgttg tccactttgt tcaccgcgag
     2401 gattaccggc gctttgccat cacgcagctt gttgagcacc atttcatcgt ccggcgtcca
     2461 gcgggtgcct tctacgacaa aaatcaccag ctcaacgtcg ccaatggagc tactcgccgc
     2521 tttgttcatc agacggttaa tagcgcgttt ttcttccata tgcagacctg gggtatcaac
     2581 gtagatcgcc tgatacgcgc cttcagtatg gatccccaca atgcggtgac gagttgtttg
     2641 cgccttgcgg gaggtaatgg agatcttctg ccccagcagt ttgttcaaca atgtggattt
     2701 gccaacgttc ggacgtccga cgatagcaat aaatccgcag taacttttat cgatgctcat
     2761 tccagctcca gttttttcaa cgcctgttcg gcggcagcct gctcagcctt acgacggctt
     2821 gaacctgtgc caaccaccgg ttcactcagg ccgctgacct ggcagtggat agtaaattcc
     2881 tgatcgtgcg cttcgccacg tacctggact accagataag ttggcagcgg cagatggcga
     2941 ccctgcaaat attcctgcag acgcgttttc ggatcttttt gtttatcgcc tgggctaatt
     3001 tcgtccagac gagtttgata ccagttgagg attaatttct cgacggtttg aatatcactg
     3061 tcgaggaata cgccaccaat taatgcctcg acggtgtcag cgagaattga ctcacgacga
     3121 aatccaccgc ttttaagttc acctggccct aaacgtaagc actcacctaa ctcaaattcg
     3181 cgcgccagtt cagccagcgt attaccacgg accagcgtgg cgcgcatccg gctcatatcg
     3241 ccttcatcca cacgagggaa acggtgataa agcgcattgg cgataacgta gctcagaata
     3301 gagtcaccta aaaattctaa acgctcgtta tgtttactgc tggcactacg atgagttaat
     3361 gcctgctgca acagttcctg atgattaaaa gtgtagccca gcttccgttg aagccgatta
     3421 attacgatgg ggttcatgcg ataccaataa ataaatgcgt caacaattca gcacacgaaa
     3481 cagacctgat atacatggct ctgctaactg cttcactgca gtttcagttt accggtatat
     3541 ggggaccaac gctgtttcgt gtgccgtggc aacctggagg tgccaacctt aaacttcggg
     3601 ggaatattct atacacaacg acgggggatg tcgttagcca cgggagattt atctcataaa
     3661 taattcacgt tgtcgccata acggcgacaa cgtgaacgaa gatggctatt aatggatacc
     3721 gccaatgcga cttaagcgca caccagtcgg ccattcgcct tcttgcttat cgaagctcat
     3781 ccagatagcc gttgcccgac cgaccagatt cgcttcaggc acaaaacccc agtaacggct
     3841 gtccgcgctg ttgtcgcggt tgtcgcccat catgaagtat tgtcccggcg gaacaatcca
     3901 ggttgccagt tgttgccctg gctgctggta atacatcccc acctggtcct gcgcaatcgg
     3961 cactgtcagg atgcggtgcg tcacatcacc caatgtctct ttacgctcgg aaagacgaat
     4021 tccattttct ttggtttcgt ttttcggcac ttcaaagaat ccgctggtcg cttccccacc
     4081 attacggcgt gagaaggtct gaacgaaatc gctcggttcc acgttggagt aggtgaccgg
     4141 gagcgcgttt tcgcacgcct ggccggaact gcatcccggt tgaatcgtca gctcttttga
     4201 gaccggatca taagtgactt tatcgcccgg caaacccacc gcgcgcttga tgtaatcaag
     4261 ctttggatct tccggatatt taaagaccac gatatcgccg cgtttcggat gaccggtttc
     4321 gatcagcgtt ttctggtaaa taggatcttt aatgccataa gcaaacttct ctaccagaat
     4381 aaaatcaccg attaagagag tcggcatcat cgaacctgac gggatctgga acggttcata
     4441 aataaacgaa cgcacaatca atacgatagc cagtaccgga aaaacagaag caccggtttc
     4501 cagccagcca ggcttcggcg caaccttttt cagcgttgct ttatccagtg agtcaccggc
     4561 agccgcctgc gccgctgcct gacgttcccg ccgtttaggt gcgaaaaaga atttatccac
     4621 gcaccataaa atgcccgtca ccagtgtggc aatcaccaga atcagggcaa acatattcgc
     4681 catggcaact cctaagggtt atttgttgtc tttgccgacg tgcagaatgg cgaggaacgc
     4741 ttcctgcggc agctcgacgt taccgatctg cttcatgcgt ttcttacctt ctttctgctt
     4801 ctgcagcagc tttttcttac gactgatatc gccgccataa catttagcca gtacgttttt
     4861 acgcagctgt ttcacggtgg agcgcgcaat gatgtgcgta ccaatcgctg cctgaatggc
     4921 gatatcaaac tgctggcgcg ggatcagatc tttcatcttc tccaccagct cgcgaccgcg
     4981 gttttgcgaa ttaccacggt gggtgatcag cgccaacgca tcaacacgtt caccgttgat
     5041 taatacgtct acacgtacca tgtcggacgc ctggaagcgt ttgaagttgt aatccagaga
     5101 cgcataacca cgcgaggtag atttcaggcg atcgaagaag tcgagcacca cttccgccat
     5161 cgggatctcg tacgtcagcg ccacctgatt accgtggtaa accatattgg tctgcacgcc
     5221 gcgtttttct acgcacagcg taataacgtt gccgagatat gcctgcggta gcagcatgtg
     5281 acactctgca atcggctcgc gcagttcgta aatgttattt accgctggca gcttggatgg
     5341 actgtcgacg tagataactt ctcttgacgt ggtttcaact tcatacacta cagtcggcgc
     5401 agtggtgatc agatccagat cgtattcacg ttccagacgt tcctggatga tctccatgtg
     5461 cagcaggcca aggaagccgc agcggaaacc aaagcccagc gcgcttgagc tttccggctc
     5521 atagaacagt gaggcatcgt tcaggctgag tttacccagc gcgtcacgga aggcttcata
     5581 gtcgtcggaa cttaccggga acagaccagc atatacctga ggtttgactt tcttaaagcc
     5641 aggcagcgcc ttttctgccg gattacgcgc cagtgttaag gtatcgccta ctggagcgcc
     5701 gtggatatct ttaatcgcac ataccagcca gcctacttcg ccacatttca gttcagtacg
     5761 gtcaacctgc ttcggcgtga agatgcccag acgatcggcg ttataggtct gcccggtgct
     5821 catgactttc actttgtcgc ccttacgcag ggtgccgttt ttaatacgga taagtgaaac
     5881 aacgcccaga tagttgtcga accaggagtc gataattagt gcctgcaacg ggccttccgg
     5941 atcgccttcc ggcggcggaa tgtcgcgcac aagacgttcg agaacgtcct gcacgccaac
     6001 gccggttttc gctgaacagc gcaccgcgtc ggtggcgtcg atacctacga tatcttcaat
     6061 ttcttccgcc acgcgttcag gatcggctgc cggcaggtca atcttgttca gtaccggaac
     6121 gacttcgaga tccatttcca ttgcggtgta gcagtttgcc agggtttgcg cttcaacgcc
     6181 ctgcccggca tcgaccacca gcagtgcgcc ttcacaggca gccagcgaac gggaaacctc
     6241 ataggagaag tctacgtggc ccggggtgtc gataaagtta agctgatagg tttcgccatc
     6301 agacgctttg tagtccagcg tcacgctttg cgctttgatg gtaatgccac gctcacgctc
     6361 aagatccatg gaatcgagaa cctgcgcctc catttcacgg tcagacaggc caccgcagat
     6421 ctggataata cggtcagaca gcgtcgattt accgtggtca atgtgagcta tgatcgaaaa
     6481 gttacgtata ttcttcataa agtatgatta ttgtgcctta atgcccggtt aaccaggctt
     6541 ttagaagtcg ctgttctgag cttaacgtct gtattaatag aaacgccgca ttctacacta
     6601 caacattgag gcgaggaaat gttcataccg tatggattgt ggtatctgga aacgtcctcg
     6661 catttgttat gcaaaatgca acaaagccag tgaaatcact ggctcgcgtc ttccgaagat
     6721 gtttcaaatc gcacaaggcc aggcggcaat gccacgctta agatgatcgg ttgccattct
     6781 gcccgggcag caaacttacg cgaatagccg cgcgcaatca ggaacccgcc aataccgccg
     6841 aggatcgcac cacataatgc tgcgacgtca gaagcaaaga gtagctgaaa tagcgaagcg
     6901 atgaggaata atcccaccag cggcgacata taaaccagta atgcggagct aagcaggctg
     6961 ccttcggcga tccccaattc cactttttgc cccggcacta acggctcatc acagggtacg
     7021 acaatggtat gcgtggtttg cgggccaagt ttattcaaca cacggctacc gcaaccggcg
     7081 cgtgaagagc agctgctgca tgaggcttta acatcacaac tgaccagcgc ctgcccgttt
     7141 tcccaggaga cgacggtagc ccactctttg atcattgcgc tgtcccgaac ttaatattct
     7201 cggcaatgcg tttcgccgtt tgcggcggca gttcaccaac aatggtgatt tcggcgttat
     7261 cacgtacgct tgtactgacg gttctgcgtc cggtgcgcaa catctgatcg gtgctcgatg
     7321 gcgtagcgcg gttaacgttt accgagaagc tgaataatcc gtcggaatag agacgtgatt
     7381 cgataggcat gttgtccatc gtcggtagcg gacgtcgact actggaaact tcgctaaaac
     7441 cctgtggcag ccaggttggc gtccagctga atttagcttt ttcaccaaca ggaacagaaa
     7501 gcaacggcgg caaatttgcc ttcgccagcg tctgcatact gctgccgata tcctgattga
     7561 cgttaaaagc aatcacgcga aattgttcca gcgtttcacc atcgcgatca aggagatcaa
     7621 cccgcatcgg taatttcgat tcggtgtcca tccacacgat gtagctgtag cgtgtaccat
     7681 ctcgggcaac cacacgaatg acttcgcaaa gacgatcagc aatacgcgtg cgcccgacag
     7741 agataaagtc gtagtaagga gaaaggcgtt tgaaatcggt atagatgagc gatggcagag
     7801 aatcaacaat gtaatcgcca ttaagcgtga acggttcaag tcccggttca aaatagctga
     7861 tttcattgcc gcgctgtacc acttcccggc gcgggccatc catttgcaac aattgtgcaa
     7921 gaggacggtt atcgaggcgt gcatgtcgat aacgcagaga ctcaacaccc tgtttattga
     7981 tgctgataaa tgacagctcg taattcagtg actgactggc caggttcatc tgctgtaata
     8041 acgccccgga cgcgggagtg gccgaggcgt tagcagagaa taacaggcta cctgtcacta
     8101 atgacatggc aaaccaaagt tgcttcatta ctgcgattgc gttcctaaag tttgaattcc
     8161 tggcacctgt acagcggctt gctgagtttg tgcctgctca aactgaagct gttcagagtg
     8221 gagtcggcgt tgcagttcgt aatcctgcaa cattgcatta atgcgacgac gctgctcctg
     8281 tacctgctgc tgttgaccat tatttgtggt cgcttcagaa ggtactccca gactaaccgg
     8341 gctggcttta cccatcatcg gcagtgtatt aaatactggc gtttcgggct gctgggacgt
     8401 ttcagattgc ccattatagt gctggacgcc aacgataact gcaagcgata cgcatgctgc
     8461 tacgcccatt tgggtaagct gtgccgccca cggacgtact ttctgccaga atggcatttt
     8521 ctgccattga tgcggcgcag gctgggcttc cgggatcaat gtcgccggtt gacgtactgg
     8581 ctcttcttca atggcggcca tcacgcgtga agagatatcg aaatggagca cctcgggagt
     8641 atcaccccgc attgagtcac ggatcaagtg atagctttcc caggttttct gcatttctgg
     8701 gttatgagcc agttcgttaa gcagctcact atccagcgtt tcgccatcca ttaaagcgga
     8761 aagttgttct ttctgcatgc ctaataccct tatccagtat cccgctatcg tcaacgcctg
     8821 ataagcggtt gaactttgtt atcaatagct tccctcgctc ggaagatacg tgaacgcacc
     8881 gtacctaccg gacaatccat gatagcggct atctcttcat agctcaggcc atccagctcc
     8941 cgcaaggtta ttgccatgcg taaatcttcc gggagggact caatagttcg gaaaactatc
     9001 tgtctcagtt cttctgacaa cattaagttc tcagggttcg aaatttcttt caacgcgccg
     9061 ccactttcga agttttcagc ttcaatggca tccacatcac tggaaggtgg acgacgcccc
     9121 tgagcaacca ggtaattttt cgctgtattt acagcaatcc gatacagcca tgtataaaaa
     9181 gcgctatctc cccggaacga atccagcgca cgataggctt taataaaagc ttcttgtacc
     9241 acatcgggaa catcacccga cggcacatag cgggaaacca gactcgccac tttatgctga
     9301 tagcgcacta ccagtaagtt aaaggctttc tgatctccct tctggacccg ttcaaccagg
     9361 acctggtccg ttaactgctc gctcatccga ggtaaagtct ccccaaacca aatttccacg
     9421 cgctatcgaa acgccactcc attagctgca atttgagcaa gcaaagggtt agagtgtctc
     9481 gtttttgtaa agttccgtaa cgcatctgtt tttgtttgtc atgctgtaga cggatcatta
     9541 tctatcatta taagtctaca gaatctgaac atcgcattat ctgtgtagaa atgcccattt
     9601 aactgcctga agagtaaccc aacggccttt ttatttcacc acctaatcct ccaccagcca
     9661 gtaacttctc gttttctcgc cgccctgagg cagcgtgttt agtcactgta acaaatatta
     9721 aaatagcagg tgtttattcg cacaacatga tgttatgctg accaaaccgt gtttagtaaa
     9781 ttaaacaaag aaaatgaata ctctccctga acattcatgt gacgtgttga ttatcggtag
     9841 cggcgcagcc ggactttcac tggcgctgcg cctggctgac cagcatcagg tcatcgttct
     9901 aagtaaaggc ccggtaacgg aaggttcaac attttatgcc cagggcggta ttgccgccgt
     9961 gtttgatgaa actgacagca ttgactcgca tgtggaagac acattgattg ccggtgctgg
    10021 tatttgcgat cgccatgcag ttgaatttgt cgccagtaat gcacgatcct gtgtgcaatg
    10081 gctaatcgac cagggggtgt tgtttgatac ccacattcag ccgaatggcg aagaaagcta
    10141 ccatctgact cgtgaaggtg gacatagtca ccgtcgtatt cttcatgccg ccgacgccac
    10201 cggtagagaa gtagaaacca cgctggtggg caaggcgcag aaccatccga atattcgcgt
    10261 gctggagcgc agcaacgcgg ttgatctgat tgtttctgac aaaattggcc tgccgggcac
    10321 gcgacgggtt gttggcgcgt gggtatggaa ccgaaataaa gaaacggttg aaacctgcca
    10381 cgcaaaagca gtggtactgg caaccggcgg tgcttcaaaa gtttatcagt acaccaccaa
    10441 tccggatatt tcttctggcg atggcattgc tatggcgtgg cgcgcaggct gccggattgc
    10501 caatctcgaa tttaatcagt tccaccctac cgcgctgtat cacccacagg cacgcaattt
    10561 cctgttaacg gaagcactgc gcggcgaagg cgcttatctc aagcgcccgg atggcacgcg
    10621 ttttatgccc gattttgatg agcgtggcga actggctccg cgcgatattg tcgcccgcgc
    10681 cattgaccat gagatgaaac gcctcggcgc ggattgtatg ttcctcgaca tcagccataa
    10741 gcccgccgat tttattcgcc agcatttccc gatgatttat gaaaaattgc tcgggctggg
    10801 gatagatctc acgaaagaac ccgtgccgat tgtgcctgct gcacattata cctgcggtgg
    10861 tgtaatggtt gatgatcatg ggcgtacgga cgtcgagggt ttgtatgcca ttggcgaagt
    10921 gagttatacc ggcttacacg gcgctaaccg catggcctcg aattcattgc tggagtgtct
    10981 ggtctatggc tggtcggcgg cagaagatat cagcagacgt ataccttatg cccacggcgt
    11041 cagtacgtta ccgccgtggg atgaaagccg cgttgagaac cctgacgaac gggtagtaat
    11101 tcagcataac tggcacgagc tacgtctgtt tatgtgggat tacgttggca ttgtgcgcac
    11161 aacgaagcgc ctggaacgcg ccctgcggcg gataaccatg ctccaacaag aaatagacga
    11221 atattacgcc catttccgcg tctcaaataa tttgctggag ctgcgtaatc tggtacaggt
    11281 tgccgagttg attgttcgct gtgcgatgat gcgcaaggag agccgtggcc tgcatttcac
    11341 gctggattat ccggaactgc tcacgcattc cggtccatcg atcctttctg ccggcaatca
    11401 ttacataaac agataaaaag cctgggtcag cgccgtatac gcttcggaat agttctggtc
    11461 tggcccacga atgactaagc gatcgctaaa gcattctccc gcctgcgggg agaatgccag
    11521 cagcacccga tgcggcagtc gcgcttcgtt ttccgccaca tccgtccgca aacgtaaatg
    11581 ccagcccata cttaatgcca gttcagtaaa accattacca atctgctctg gcagcaccac
    11641 gcagaaaaat ccctcttcgg taatgcactc cgccgcacag gtcagcaacg atgggtgatc
    11701 aagcgtagtg gtatagcgag cctgttcccg ttgaggtgtc gagcactcta ctccctgctg
    11761 atagtaaggt gggttactga tgattaaatc gaagcgtact gtctgctgtg tgatccactg
    11821 ctgaatatcc gccgtatgga cgttaatccg ctctgcccac ggggactggt tgatattttc
    11881 ctgcgcctgc gcggcagctt cactttccag ttcaactgca tcaatcatta cgctgtcatc
    11941 ggttcgctgc gccagcatta atgccagcaa cccgctaccc gcgccgatat caaggcagcg
    12001 ttttacccca gccaccggtg cccatgcgcc cagcaaaatg ccatccgttc ccactttcat
    12061 cgcacaacga tcgtgagcaa caaaaaactg tttaaaagta aatccattac gacgaagcac
    12121 ggatgtagac tgtgacatga aaataaaacc ttgcaggaaa aacggcgata gcaccgggtg
    12181 agaacaatac ccgagaagcg atatccatac aaacagatga agattgcagc cgtaacgtct
    12241 ataatcagcg ccccacacag aggtagaaca tgactgtaac gactttttcc gaacttgaac
    12301 tcgacgaaag cctgctggaa gccctccagg ataaaggttt cactcgcccg accgccattc
    12361 aggctgccgc cattccgcct gcgctcgatg gccgtgatgt actcggttct gcgccgacag
    12421 gcaccggtaa aacggcggcg tatctgctgc cagcgttgca gcacctgctc gatttcccac
    12481 gtaagaaatc cggtccgccg cgtattttga tcctcacccc aactcgcgag ctggcgatgc
    12541 aggtggccga tcatgcccgc gaactggcga aacatacgca tctggatatc gccaccatca
    12601 ccggcggcgt agcctatatg aaccacgcgg aagtgttcag cgaaaatcag gacatcgtgg
    12661 tcgccacgac cggacgtctg ctgcaataca taaaagaaga gaacttcgat tgccgcgcgg
    12721 ttgaaacgct gatcctcgac gaagcagacc gtatgctgga tatgggcttc gcccaggata
    12781 tcgaacatat tgctggcgaa acgcgctggc gtaaacagac cctgctcttt tcggcaactc
    12841 tggaaggcga tgcgattcag gactttgccg agcgtctgct ggaagatccg gtggaggttt
    12901 ctgccaatcc gtccacccgt gagcgcaaaa aaattcatca gtggtattac cgcgccgatg
    12961 atcttgagca taaaaccgcg ttgctggtgc atctgttaaa acagccggaa gcgacccgct
    13021 caattgtgtt tgtgcgtaag cgtgagcgtg tgcatgagct ggcaaactgg ctgcgcgaag
    13081 caggcatcaa caactgctat ctcgaaggtg agatggtaca aggcaagcgt aacgaagcga
    13141 ttaaacgctt gaccgaaggc cgcgtaaacg tactggttgc taccgatgtt gccgcgcgcg
    13201 gtatcgacat tcctgacgtc agccacgtct ttaacttcga tatgccgcgc agtggcgata
    13261 cttatttgca ccgtatcgga cgtaccgcgc gcgccggtcg taaaggcacc gcgatttcgc
    13321 tggtggaagc ccatgaccat ctgctgctgg gcaaagttgg ccgttatatt gaagagccaa
    13381 ttaaagctcg cgtaattgat gagttacgtc cgaaaacgcg tgcgccaagc gagaagcaga
    13441 ccggcaagcc atcgaagaaa gtgttggcta aacgtgccga gaagaaaaaa gctaaagaga
    13501 aagagaagcc gcgggtgaaa aaacgccatc gcgacactaa aaatattggt aagcgccgta
    13561 aaccaagcgg aacgggcgtg ccaccgcaaa cgacagaaga gtaatctcaa tgccgggttt
    13621 aagcccggca ttgtttttat cccggcaaaa aacactcttc aacacactga ataaaagtgt
    13681 gcatcgccgg gctaaccgct tttccggcat ggtgagcgca catggccgta atggtttgcg
    13741 actgttcgcc aaaaggcaat tcaattaatt ctccgcactc caactctttt accaccgcaa
    13801 aacgcggcag gtaactgact ccgatatttg ccgcgacaca acgcttgatg ctttcgatac
    13861 ttataagctc aatggtgttt tccaccgtga tccgccgctg gtgcagcgtg ctctcaaata
    13921 tctgccggaa gacacattgt ggttcgttga taataaagct acaggcgtta tgtcttcccg
    13981 gctcagtaaa atcgacatct gcaatttgcg gtgaagccac cagcaccagc ggttgttcac
    14041 ccaactctcg tcggttcaaa gcatcatcat tccctacgcg ataaaaaacc ccgacatcgg
    14101 cctcatcatt cagtagcgca tcacggatca cgtaacagtt cagcgactgc aacgataaac
    14161 gcacttttgg agcgcgttgc cgaaaacgct gcaacacctg cggcattcga taagagagca
    14221 acgtttcgcc tgaaataacg cgtagttccc cgtccggatc cgactctttt ttggcagcct
    14281 cacgaagggt atccatcact ctggtgagtt cataaatgtg cggcagcagc ttttttcctt
    14341 cgcgggtaag gcacattcgt cgaccaattt tctcaaataa ctggactgag aactcctgct
    14401 caagctgctg aatatgaaaa gtcaccgtcg attgtgtgca gcacaatttt tgcgaagctc
    14461 gcaaaaagga accctcttcc accacggttt taagtgtaat aaaacggcgc agatccataa
    14521 ccccaaacct atcgaaaata tcgaatttag aatgtaaaaa cattcatttt tttaaatgtt
    14581 ccgtgtcggg tactgtctac caaaacagag gagataacaa gtgacaccga cccttttaag
    14641 tgctttttgg acttacaccc tgattaccgc catgacgcca ggaccgaaca atattctcgc
    14701 ccttagctca gccacaacgc acggatttcg tcagagtact cgcgtgctgg cagggatgag
    14761 tctgggattt ttaatcgtca tgttgctatg cgcgggaatt tcgttttcac tggctgttat
    14821 cgacccggca gcagtgcatc tgttgagttg ggcgggggcg gcatatattg tctggctggc
    14881 gtggaaaatc gctaccagtc caacaaagga agatgggctt cagacaaaac caatcagttt
    14941 ttgggccagc tttgctttgc agtttgtgaa cgtcaaaatc attttgtatg gcattacagc
    15001 actgtcgacg tttgtcctgc cgcaaacgca ggcgctaagc tggataattg gcgtcagcgt
    15061 tttgctggcg gtgattggga cgtttggcaa tgtgtgctgg gcgctggcgg ggcacctgtt
    15121 tcagcgattg tttcgccagt atggtcgcca gttaaatatc gtgctcgcgc tgctgctggt
    15181 ctattgcgcg gtacgcattt tctattaatg aaaaaagcgg aagaggtcgc cctcttccgc
    15241 ttagtaactt gctacttaag ccttacaggc tttcagtaaa ggtacgagcg ataacgtcgc
    15301 gctgctgttc cggagtcaga gagttaaagc gaactgcata accggataca cggatggtca
    15361 gctgcggata tttttccgga tgcttaactg catcttccag agtttcacga cgcagaacgt
    15421 ttacgttcag gtgttgacca ccttcaacgc gaacttctgg tttcacttct actggaactt
    15481 cacggtactc aatgtcaccc agtttgctta ctgcaaccac ttcgtcttct gcaaaacctg
    15541 ctttcgcaac gatgcaacgc gcttcgcctt tttcgctgtc cagcagccag aaagagttca
    15601 gcagatcgtc gttagcggct ttagtaatct ggatacctgt aatcatgtga tgcctccccg
    15661 gcaaaattat ttgatttgtt cagcctgtcg cggccaattg gtaaaaccat tgttgcttga
    15721 gtgtatatat actcctcaaa cacccttgaa tctttgattt aaatcaataa aaaccacaca
    15781 tcaagtatgg ttgcaaatgg attttattgt tttacatcaa cttatgcagg tgtgaaattt
    15841 taccaattta catttttttg cactcgttta agtctaaaaa atgagcatga ttttgttctg
    15901 tagaaagaag cagttaagct aggcggattg aagattcgcg ggagagcgag atggcaaacg
    15961 aattaacctg gcatgatgtg ctggctgaag agaagcagca accctatttt cttaataccc
    16021 tacagaccgt cgccagcgag cggcagtccg gcgtcactat ctatccgcca caaaaagatg
    16081 tctttaacgc gttccgcttt acagagttgg gtgacgttaa agtggtgatt ctcggccagg
    16141 atccttatca cggaccggga caggcgcatg gtctggcatt ttccgttcgc cccggcattg
    16201 cgacaccgcc gtcactgctg aatatgtata aagagctgga aaatactatt cccggtttca
    16261 cccgccctaa tcatggttat cttgaaagct gggcgcgtca gggcgttctg ctactcaata
    16321 ctgtgttgac ggttcgcgca ggtcaggcac attcccacgc cagcctcggc tgggagacct
    16381 tcaccgataa agtaatcagc ctgattaacc agcaccgcga aggcgtggtg tttttgttgt
    16441 ggggctcaca tgcgcaaaag aaaggggcga ttattgataa gcaacgccat cacatactga
    16501 aagcgccgca tccatcaccg ctttctgcgc atcgtgggtt ctttggctgc aaccattttg
    16561 tgctggcaaa tcagtggctg gaacaacgtg gcgagaaacc gattgactgg atgccagtat
    16621 taccggcaga aagtgagtaa atttgcagga aaacgccgga tggcagcgtt gccacccggc
    16681 tgatttatca ggctttattc tgacgccacc attcaccaag caaaacgccg gttgcaacag
    16741 agatatttag cccggcaacg ttgccagtac catcaatctt cacgcgcaaa tcgttcggat
    16801 cgcgtgcggc atccggtaac ccttcatatt cctgacccag caccagtacc attttcgctg
    16861 gcagactggt tttgaacagc ggtttcccct gctcgctgga agtggtcact acggtgtaac
    16921 ctgcctgacg gaaatcatcc agcacgttaa caatgttgtc gccagtaatc ggctgaacgt
    16981 gctctgcgcc gccttctgcg gtacggatag ccgcccccga ctccagcagt gccgcatcct
    17041 gcaccacaac gcctttcacg ccgaagtgcg cgcagctgcg catcatgcca cccaggttat
    17101 gcgggttaga ttcgttttcc agcgccagta cacaatcctg cgcgcctgcc tggcttaccc
    17161 actgcagcac ggttgtaccg ttacgctttt tgatcaggaa gcaaacgccg ccgtgatgtt
    17221 ccgtgcctga cgcttttgtc agttccgctt catctaccac atggtacgct ttgcggtttg
    17281 ctgccatcca gcgcaaggct tctttaaaac gcggcgttac actctggata aaccaggcgc
    17341 gaacaatcgc ttccggacgg ctctggaaca gtgcctgaca ggcattttcg ccgtagacgc
    17401 gggtttcttc cgcacgctga cgacgcaaca cttccggatc aataaaactt ttaccactga
    17461 tgccaccgtg atcggccttt tccggcgtct catcacccgg cgcgcgggaa actgtgcgcc
    17521 acggcgactc ttcccacttg cggtcacggg gctgactctg tttgtcatcg cgggcggggc
    17581 gacggccacc gtcagcacga gattttcctg gacgcccgcc ccctttcccg gtacgcgggt
    17641 tgtgggtacg tttatcagaa tcatcatcac tgcggacata catcactttg accttgccgc
    17701 ttttaccttt catttcatcg ttcatgcttt tctccaccag cgctgcgcga agcgcgcaga
    17761 ttacccgaag tccgcgcggt tcgccatgat ttcgtaccaa agcctgcgac tatcatacct
    17821 attgaataaa acagattgtt gtctggaaca atgtccccga taatatgtaa catattagaa
    17881 acataccggc gtcgttgccg ataagtctcc ttactcatcc cgaggttagt tatgaatacc
    17941 gtttgtaccc attgtcaggc catcaatcgc attcccgacg atcggatcga agatgcggca
    18001 aaatgcggac gctgcggtca cgacttgttt gacggagagg tgattaatgc gaccggtgaa
    18061 acgctcgaca aattgctgaa agatgatcta cctgtggtga tcgacttctg ggcaccgtgg
    18121 tgcggcccct gccgtaattt cgcaccaatt tttgaagatg tcgcgcaaga gcgtagcggt
    18181 aaagtgcgct ttgtgaaagt gaataccgaa gctgaacgtg aattgagcag tcgctttgga
    18241 attcgtagta taccgacgat catgattttc aaaaacggtc aggttgtcga catgcttaat
    18301 ggcgcagtac cgaaagcgcc gtttgatagc tggctgaacg aatctcttta atcttaccgg
    18361 ggcgcatctt gtgccccgtt ttctcctctg cgacaatggc gttttttcga cgctctctta
    18421 tgaccgaaaa cgctgttctc cagttacgcg ccgagcgtat tgcgcgcgca acacgtcctt
    18481 ttcttgcccg cggtaatcgc gttcgtcgct gccaacgctg tcttttgcca gagacattat
    18541 gtctctgttc gacaattacc ccagcacaag caaaaagtcg cttctgtttg ctgatgttcg
    18601 acaccgagcc aatgaagccc agtaataccg ggcgtctgat tgctgatatt ttgcctgata
    18661 ccgttgcgtt tcaatggtcg cgtaccgaac cctcgctaga tttgctggat ctggtacaaa
    18721 acccggacta tcagccaatg gtggtctttc ccgcctcgta tgctgatgag caacgggaag
    18781 tgatcttcac accacctgcc ggtaagcctc cgctatttat catgctcgat ggtacctggc
    18841 ctgaagctcg caagatgttt cgtaaaagtc cgtatctgga taatcttccc gtcatttccg
    18901 tcgatctttc ccggctttct gcctatcgcc tgcgtgaagc ccaggctgaa ggccaatatt
    18961 gtactgccga ggtagccatc gcactgttag atatggccgg cgataccgaa gccgcaacgg
    19021 gcttaggcga gcatttcact cgttttaaaa cgcgctatct ggcaggaaaa acgcaacatc
    19081 tgggtagcat cacagcagaa cagttagaaa gcgtttaaaa tcattcggtc acttctgcgg
    19141 gagaccggta tgagtcagcg aggactggaa gcactactgc gaccaaaatc gattgcggta
    19201 attggcgcat cgatgaaatc caatcgagca ggttacctga tgatgcgtaa cctgctggcg
    19261 ggaggcttta acggaccggt actcccggtg acgccagcct ggaaagcggt gttgggtgtg
    19321 ttggcctggc cggatattgc cagcttgccc tttacacccg accttgcggt tttatgtacc
    19381 aatgccagcc gtaatcttgc tcttctggaa gagctcggcg agaaaggctg taaaacctgc
    19441 attattcttt ccgccccggc atcgcaacat gaagatctcc gcgcctgcgc cctgcgccat
    19501 aacatgcgcc tgctaggacc aaacagtctg gggttactgg ctccctggca aggtctgaat
    19561 gccagctttt cgcctgtgcc gattaaacgc ggcaagctgg cgtttatttc gcaatcggct
    19621 gccgtctcca ataccatcct cgactgggcg caacagcgtg agatgggctt ttcctacttt
    19681 attgcgctcg gcgacagcct ggatatcgac gttgatgaat tgctcgacta tctggcccgc
    19741 gacagtaaaa ccagcgccat cctgctctat ctcgaacagt taagcgacgc acgacgcttt
    19801 gtttcggcgg cccgtagtgc ctctcgcaac aaaccgattc tggtgattaa aagcggacgt
    19861 agcccggcgg ctcaacgtct gttgaacacc acggcaggaa tggatccggc gtgggatgcg
    19921 gctattcagc gtgccggttt gttgcgggta caggatactc acgagctgtt ttcggcggtg
    19981 gaaaccctca gccatatgcg cccgctgcgt ggcgaccggc tgatgattat cagcaacggt
    20041 gctgcgcctg ccgcgctggc gctggatgcc ttatggtcac gcaatggcaa gctggcaacg
    20101 ctaagcgaag aaacctgcca gaaactgcgc gatgcactgc cagaacatgt ggcaatctct
    20161 aacccgctcg atctacgcga tgacgccagc agtgagcact atattaaaac gctggatatt
    20221 ctgctccaca gccaggattt tgacgcgctg atggttattc attcgcccag cgccgctgct
    20281 cccgcaacag aaagtgcgca agcattaatt gaagcggtaa agcatcatcc ccgcagcaag
    20341 tatgtttctc tgctgacgaa ctggtgcggc gagcactcct cgcaagaggc acgacgatta
    20401 ttcagtgaag ccgggctgcc gacctatcgc acgccggaag gaaccatcac tgcttttatg
    20461 catatggtgg agtaccgacg taatcagaag caactacgcg aaacgccggc gttgcccagc
    20521 aatctgacct ctaataccgc agaagcgcat cttctgttgc aacaggcgat tgccgaaggg
    20581 gctacgtcgc tcgataccca tgaagttcag cccatcctgc aagcgtatgg catgaacacg
    20641 ctccccacct ggattgccag cgatagcacc gaagcggtgc atattgccga acagattggt
    20701 tatccggtgg cgctgaaatt gcgttcgccg gatattccac ataaatcgga agttcagggc
    20761 gtaatgctct acctgcgtac agctaatgaa gtccagcaag cggcaaacgc tattttcgat
    20821 cgcgtaaaaa tggcctggcc gcaggcgcgg atccacggcc tgttggtgca aagtatggct
    20881 aaccgtgctg gcgctcagga gttgcgggtt gtggttgagc acgatccggt tttcgggccg
    20941 ttgatcatgc tgggcgaagg cggtgtggag tggcgtcctg aagatcaagc cgtcgtcgca
    21001 ctaccgccgc tgaacatgaa cctggcccgc tatctggtta ttcaggggat caaaagtaaa
    21061 aagattcgtg cgcgcagtgc gctacgccca ttagatgttg caggcttgag ccagcttctg
    21121 gtgcaggttt ctaacttgat tgtcgattgc ccggaaattc agcgtctgga tattcatcct
    21181 ttgctggctt ctggcagtga atttaccgcg ctggatgtca cgctggatat cgcgccgttt
    21241 gaaggcgata acgagagtcg gctggcagtg cgcccttatc cgcatcagct ggaagaatgg
    21301 gtagaattga aaaacggtga acgctgcttg ttccgcccga ttttgccaga agatgagcca
    21361 cagcttcagc aattcatttc gcgagtcacc aaagaagatc tttattaccg ctactttagc
    21421 gagatcaacg aatttaccca tgaagattta gccaacatga cgcagatcga ctacgatcgg
    21481 gaaatggctt ttgtggcggt acgacgtatt gatcaaacag aagagatcct cggcgtcacg
    21541 cgtgcgatct ccgaccctga taacatcgat gccgaatttg ccgtgctggt tcgctcggat
    21601 ctcaaagggt taggcttagg tcgacgctta atggaaaagt tgattaccta tacgcgagat
    21661 cacggactac aacgtctgaa tggtattacg atgccaaaca atcgtggcat ggtggcgctg
    21721 gcccgcaagc tcgggtttaa cgttgatatc cagctcgaag aggggatcgt tgggcttacg
    21781 ctaaatcttg cccagcgcga ggaatcatga gtaaggtact ggaaatgttg accacttaat
    21841 cgggactggt gttattattg cccgcttatg tcgtctgcat tgcacagagg acccttcaat
    21901 gaacagagaa gaaatgcact gtgatgttgt caaaatttaa gcgtaataaa catcaacaac
    21961 accttgccca actacccaag atttctcaat cagttgatga tgtcgatttc ttttacgctc
    22021 ccgccgactt ccgggagacg ttgctggaaa aaatagccag cgcgaagcag cgcatttgca
    22081 ttgttgccct gtatctcgaa caggatgacg gtggcaaagg cattctgaac gcgttgtatg
    22141 aggctaaaag gcagcgtccg gaactggatg tgcgggtgct ggtcgactgg catcgtgcac
    22201 aacgtggacg cattggcgct gcggcatcta acactaacgc tgactggtac tgccgcatgg
    22261 cgcaggaaaa tccgggcgta gatgttccgg tttatggcgt tccaatcaat acccgtgaag
    22321 cccttggtgt tctgcacttt aaaggcttta tcatcgacga tagcgtactt tatagcggtg
    22381 ccagcctgaa cgatgtttac ctgcatcagc acgataaata tcgctacgac cgttatcatc
    22441 tgatccgtaa ccgtaagatg tcagacatta tgtttgaatg ggttacacag aatattatga
    22501 atggccgcgg cgttaatcgt ctggatgatg ttaatcggcc aaaaagcccg gaaatcaaga
    22561 acgacattcg cctgttccgc caggagctgc gtgatgccgc ttatcatttc cagggcgatg
    22621 ccgacaacga tcagctttcc gtaacgccgc tagtggggct ggggaaatcg agtctgttga
    22681 acaagaccat tttccatctt atgccttgtg ccgagcagaa actaaccatc tgtacgccat
    22741 acttcaacct gccagcaatc cttgtgcgca atattatcca gttgctgcgc gaagggaaaa
    22801 aggtcgaaat tattgttggt gataaaaccg cgaacgactt ctacattccg gaagatgaac
    22861 ctttcaagat aattggcgca ttgccttatc tctatgagat caatctgcgt cgtttcctga
    22921 gccgtttgca gtattacgtc aatactgacc agctagtggt tcggttatgg aaagatgacg
    22981 acaacaccta tcacctaaaa gggatgtggg ttgatgataa gtggatgttg atcaccggta
    23041 ataacctgaa cccgcgcgcc tggcgtctgg atctggaaaa cgccattttg atccacgatc
    23101 cgcaactgga gcttgcgcca cagcgagaga aagaactgga gctgatccgc gagcatacca
    23161 ccatcgttaa gcactatcgc gatctgcaaa gtattgccga ttatccggtg aaggttcgta
    23221 aactcatccg tcgtttgcgc cgtatccgca tcgaccgatt aattagccgc atcctgtaat
    23281 cacaaccccg tcctgtacgg ggtttgtttt ggaggccacg ttttgcgtat tctttttgtc
    23341 tgttcgctat tgttactttc tggatgcagc catatggcta acgatagctg gagcgggcag
    23401 gataaagctc aacactttat cgcctcggcg atgctttccg ccgccggaaa tgaatatgca
    23461 cagcatcagg ggatgagtcg cgatcgcagt gccactttcg ggctgatgtt ctctgttggg
    23521 ctgggcgcgt caaaagaact ttgggatagc cgccccgagg ggagcggctg gagctggaaa
    23581 gattttacct gggatgtcgc cggtgcaact actggctata ccgtctggca actgacccgt
    23641 cactaaagac gcatcccctt ccctttgcga tgtagcatca aagaaaccag aaacgccacc
    23701 acggccatca aggtcacata ccagaagaag gctgtttcca ttcctattga tttcagcgac
    23761 aacgctacgt actccgccga accaccaaat atagcattgg cgaccgcata tgacagacca
    23821 acacctaatg cacgaacctg tgccgggaac atctcagcct tcagtattcc actgattgaa
    23881 gtataaaaac tcacgatcag cagggcacac atcaccagac caaaagcggc ataaggcgag
    23941 gaaacgtttt gtaatgctga gagaatagga acggtaaaaa tggctgccag cgaaccgaaa
    24001 cataacattg aggtacggcg accaatctta tccgacagcg cgccaatgag tggttgaata
    24061 agcatgaata caaacaatgc ggcagtcata atgccactcg ccacgttggc atgcattccc
    24121 gcagtattta ccagatactt ctgcatataa gtggtgaagg tatagaaaca aagggagccc
    24181 gcagcggtaa aaccgagaac catgatgaat gcacggcgat tgcgccataa tcctttcaga
    24241 gatccagctt cttttaaagc gcgcgtttct tgttgcgaag tttcatctaa ctgacgacgt
    24301 aaccacaacg ccacaacagc taacacagct cctaacgcga aaggaatacg ccatccccac
    24361 tctctgagtg cagcgtcttc catggtatgt tgtaaaacca cgacaaccag tagggctagc
    24421 agttgtccgc cgatcaacgt cacatactga aatgatgcgt aaaaaccttt gcgcccttca
    24481 acggcaactt cactcatata ggtggcgctg gtgccatatt ctccgccaac ggataatccc
    24541 tgaaataaac gagcgagaag caataatgcc ggagcccacg tacctatagt ttcataacct
    24601 gggaggcagg cgataaccag cgatccgaaa cacatcatac acaccgataa cagcatcgat
    24661 tttttgcgac catgtttatc ggctatgcgg ccaaatagcc aaccgcctat tgggcgcatc
    24721 aggaatcccg cagcaaaaac acctgctgtt tgtagtagtt gagtcgtcgt gttcccggaa
    24781 gggaagaaaa tgtgggcgaa gtagagtgaa cagaacgagt agacatagaa atcgaaccac
    24841 tcgaccagat tacctgaaga ggcccccaca attgcccaaa tgcggcgacg agtatcacta
    24901 cttgtcagtt tgctgtctgc cgttacagta ctttcagcca tgccattatg tctcctgccg
    24961 taatccgatg cttttgtcgg tcgcttttgt ttatttctat tggtaaagaa aatgttatac
    25021 atttgttgca tatcattatg caaccttaac cacgaattta gttagcagga aaatggttat
    25081 tgaggagctt aaggataaat ttctggtaag gaggacacgt atggaggtgg gcaagttggg
    25141 gaagccgtat tcgctgctga atctagctta tgtgggagta taagacgcgc cagcgtcaca
    25201 tccggcattt attccgcacc agcgcaaaaa ggccatccgt caggatggcc ttctgcttaa
    25261 tttgatgcct ggcagttccc tactctcgca tggggagacc ccacactacc atcggcgcta
    25321 cggcgtttca cttctgagtt cggcatgggg tcaggtggga ccaccgcgct aaggccgcca
    25381 ggcaaattct gttttatcaa cacgtctcgt tatgacctgt cgatttaatc tgtatcaggc
    25441 tgaaaatctt ctctctatcc gccaaaacat cttcggcgtt gtaaggttaa gcctcacggt
    25501 tcattagtac cggttagctc aacgcatcgc tgcgcttaca cacccggcct atcaacgtcg
    25561 tcgtcttcaa cgttccttca ggagacttaa agtctcaggg agaactcatc tcggggcaag
    25621 tttcgtgctt agatgctttc agcacttatc tcttccgcat ttagctaccg ggcagtgcca
    25681 ttggcatgac aacccgaaca ccagtgatgc gtccactccg gtcctctcgt actaggagca
    25741 gcccccctca gttctcca
//
LOCUS       DAZFJL010000065        25463 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFJL010000065 DAZFJL010000000
VERSION     DAZFJL010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.065"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(84..1367)
                     /gene="yeaH"
                     /locus_tag="ACHJ8C_002567"
     CDS             complement(84..1367)
                     /gene="yeaH"
                     /locus_tag="ACHJ8C_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO5552408.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKILPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1480..3414)
                     /gene="yeaG"
                     /locus_tag="ACHJ8C_002568"
     CDS             complement(1480..3414)
                     /gene="yeaG"
                     /locus_tag="ACHJ8C_002568"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO5552409.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            3850..4596
                     /gene="mipA"
                     /locus_tag="ACHJ8C_002569"
     CDS             3850..4596
                     /gene="mipA"
                     /locus_tag="ACHJ8C_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO5552410.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            4686..5540
                     /gene="yeaE"
                     /locus_tag="ACHJ8C_002570"
     CDS             4686..5540
                     /gene="yeaE"
                     /locus_tag="ACHJ8C_002570"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO5552411.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALIGLRENVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFALEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQKQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATVAHVQQNAAVLEVELSSAELVMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5591..6475)
                     /gene="yeaD"
                     /locus_tag="ACHJ8C_002571"
     CDS             complement(5591..6475)
                     /gene="yeaD"
                     /locus_tag="ACHJ8C_002571"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO5552412.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFHVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6559..7554)
                     /gene="gapA"
                     /locus_tag="ACHJ8C_002572"
     CDS             complement(6559..7554)
                     /gene="gapA"
                     /locus_tag="ACHJ8C_002572"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO5552413.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            7896..8309
                     /gene="msrB"
                     /locus_tag="ACHJ8C_002573"
     CDS             7896..8309
                     /gene="msrB"
                     /locus_tag="ACHJ8C_002573"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO5552414.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8351..8623
                     /gene="yeaC"
                     /locus_tag="ACHJ8C_002574"
     CDS             8351..8623
                     /gene="yeaC"
                     /locus_tag="ACHJ8C_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO5552415.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVTLTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            8993..10069
                     /gene="ydjL"
                     /locus_tag="ACHJ8C_002575"
     CDS             8993..10069
                     /gene="ydjL"
                     /locus_tag="ACHJ8C_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO5552416.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            10096..11475
                     /gene="ydjK"
                     /locus_tag="ACHJ8C_002576"
     CDS             10096..11475
                     /gene="ydjK"
                     /locus_tag="ACHJ8C_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5552417.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWSYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            11492..12535
                     /gene="ydjJ"
                     /locus_tag="ACHJ8C_002577"
     CDS             11492..12535
                     /gene="ydjJ"
                     /locus_tag="ACHJ8C_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFO5552418.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLTMAEQLGATVVI
                     NGVKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            12556..13392
                     /gene="ydjI"
                     /locus_tag="ACHJ8C_002578"
     CDS             12556..13392
                     /gene="ydjI"
                     /locus_tag="ACHJ8C_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO5552419.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALDKIKLFGSDGK
                     AE"
     gene            13397..14344
                     /gene="ydjH"
                     /locus_tag="ACHJ8C_002579"
     CDS             13397..14344
                     /gene="ydjH"
                     /locus_tag="ACHJ8C_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO5552420.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGRALTEIFTQA
                     KARQMIICADMIKPRLNETLNDICEALSYVDYLFPNFAEAKLLTGKEALDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSIGATTGVKNRKLVEQLLEEYEG"
     gene            14354..15334
                     /gene="ydjG"
                     /locus_tag="ACHJ8C_002580"
     CDS             14354..15334
                     /gene="ydjG"
                     /locus_tag="ACHJ8C_002580"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFO5552421.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNALKAE
                     GKIRAIGAANVDADHIREYLQHGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            15471..16229
                     /gene="ydjF"
                     /locus_tag="ACHJ8C_002581"
     CDS             15471..16229
                     /gene="ydjF"
                     /locus_tag="ACHJ8C_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5552422.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            16347..17705
                     /gene="ydjE"
                     /locus_tag="ACHJ8C_002582"
     CDS             16347..17705
                     /gene="ydjE"
                     /locus_tag="ACHJ8C_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5552423.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMHWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPPLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(17798..18439)
                     /gene="pncA"
                     /locus_tag="ACHJ8C_002583"
     CDS             complement(17798..18439)
                     /gene="pncA"
                     /locus_tag="ACHJ8C_002583"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO5552424.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLKQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRAHVINELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(18450..19466)
                     /gene="ansA"
                     /locus_tag="ACHJ8C_002584"
     CDS             complement(18450..19466)
                     /gene="ansA"
                     /locus_tag="ACHJ8C_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO5552425.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(19633..21489)
                     /gene="sppA"
                     /locus_tag="ACHJ8C_002585"
     CDS             complement(19633..21489)
                     /gene="sppA"
                     /locus_tag="ACHJ8C_002585"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO5552426.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSSAAREADSRWIGELWKNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            21650..22201
                     /gene="ydjA"
                     /locus_tag="ACHJ8C_002586"
     CDS             21650..22201
                     /gene="ydjA"
                     /locus_tag="ACHJ8C_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO5552427.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDNKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            22318..23361
                     /gene="selD"
                     /locus_tag="ACHJ8C_002587"
     CDS             22318..23361
                     /gene="selD"
                     /locus_tag="ACHJ8C_002587"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO5552428.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            23366..25312
                     /gene="topB"
                     /locus_tag="ACHJ8C_002588"
     CDS             23366..25312
                     /gene="topB"
                     /locus_tag="ACHJ8C_002588"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO5552429.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQARSTPVRRFRGIVASGGGA
                     VKKAPNKRVKKAAKPKTDNTDSAN"
BASE COUNT         6420 a         5932 c         6545 g         6566 t
ORIGIN      
        1 attgtaagct aatgtatgta ataaatgaga taatttacag tgagctaaaa atcagccagg
       61 cgataatgac ccggctgaca gttttagtct ttagctgttg cattttgttt atgaaacagt
      121 tcacggaaca ccggataaat atcatcctgg tcgcggatat gctgcatcgc aaagttgtcg
      181 aaagtagatt gcagatgctc atattctcgc cacagtgtct gatgcgcgcg acgtgtaatt
      241 tcgatatagc tgtaataacg aacaacgggt aatatttttt tcgccagaat ttcatggcaa
      301 agcggagagt catcggccca gttatcgccg tctgatgctt gggctgcgta aatattccac
      361 tgtgccgggt tataacgctc tttcactacc tcatccatca gtttcagggc gctggaaaca
      421 atggtgccgc cagtttcctg cgagtagaaa aactcatgtt catcgacttc tttcgcctgg
      481 gtatgatggc ggatgtatac gacttccacg ttcttatacg ttctgctgag gaacagatac
      541 agcagaatat aaaaacgttt agccatatcc ttagtggact gatccattga accggaaacg
      601 tccatcaggc aaaacatcac tgcctggctg gagggatcgg gccgtttttc gtagttcttg
      661 taacgtaaat cgaaagtgtc aatgaaagga acgcgttcaa ttttggcgcg caattcagca
      721 atctctttgc gcagccgttc ctcttccagc agttgcgcag gttcactgtt gctgatgatg
      781 gccaaattct cttccagtgc atgaagttcc cgccgcttgc ccgccgtcat ggctgtgcgc
      841 cgcgccagtg agttctgcaa tgaacgtaca acgctgatat tggccggaac gccgttagcg
      901 gtataacccg cccgatgcgt tttatattcg gtcagctggc gttgttggtt ttgtttcaga
      961 ttcggtaagg ccaaatcttc aaagagcaga tcaagatact catctttcga aatctgaaag
     1021 acaaattcat cctgaccttc accatcctgg ctggcctggc cctgaccact gccggaacct
     1081 ccgccaccac cctggggacg ttcaattcgg tcgttctgga cgaaatggtc attgcccgga
     1141 tgcacgcggt ggcgcagacc gccacgcccc tgatgaaaca tcggttcgct aatatcttcc
     1201 gtgggaatgg atacggactc gccgctgtcg acgtcagtca ccgaacgctt attaatggcc
     1261 tcggagatcg actgtttaat ttgcgcttta taacggcgta aaaaacgctg gcgattcacc
     1321 atgcttttgt ttttgccgtt cagacgccgg tcaataaacc aggtcatatg ccccccgtac
     1381 tacatttgcc aactttatgc tacgcctgtg ttgccgcttc tctggcccac ggttcgctgt
     1441 cgttgtaggc ccggtagcat gtgtaccggg ccagggttgt taagacgatt tacgtacgcg
     1501 caaataccat tcgcacagta aacgcacctg tttacgggtg tagccttttt ccatcatacg
     1561 gtcgacaaag tcgtcatgtt tcttttgctc gtcggttgac gttttggcgt taaacgaaat
     1621 aaccggcaac agctcctcgg tattggagaa cattttcttc tcaataaccg tgcgcagttt
     1681 ttcatagctg gtccagttcg gattgcgtcc gctgttattc gctctggctc gcagtacgaa
     1741 gttaacaatc tcgttgcgga aatcttttgg attactgatc cccgccggtt tctcgatttt
     1801 ctccagctcg gcgttaagag actcgcggtc aaacaactgc ccggtatccg gatcgcgata
     1861 ctcctgatcc tgaatccaga aatccgcgta ggtaacataa cggtcaaaaa tgttctgccc
     1921 atattctgaa taggattcaa ggtaggccgt ctggatctct ttgccgataa actcggcata
     1981 tttcgggatc agataacctt tcaggaactc cagatagcgt tctgcctgct cttgcgggaa
     2041 ctgctcgcgc tcgatctgct gttccaggac gtagaacaga tggaccgggt ttgccgccac
     2101 ttctacatga tcgaagttga acacgcggga gaggatctta aacgcaaaac gcgtcgacag
     2161 accgttcatc ccttcatcga caccggcgta gtcacgatat tcctgatacg acttggcttt
     2221 gggatcagtg tctttcaaac tttcaccatc ataaacccgc atctttgaat aaatgctgga
     2281 gttttctggc tctttcagac gggaaagaat agaaaaacgt gacagcgttt ccagcgtgcc
     2341 aggggcgcag ggggcgtgag tcaattcact gtgattaagc aatttctcgt agattttgat
     2401 ctcttcggaa atgcgcaagc aatacggcac cttcacgatg taaacacggt cgaggaaggc
     2461 ttcgttgttt ttgttattac ggaaagtgac ccattcggat tcgttcgagt gggcgagaat
     2521 aatcccgttg aacggcaggg cggagatacc ttccgtcccg ttgtagttac cttcctgggt
     2581 ggcggttaac aagggatgca gcacttttat cggtgcttta aacatctcga caaattccat
     2641 gatcccctga ttggcgcggc acagcgcacc ggaatagccg taggcgtccg ggtcattctg
     2701 cgcgtagtgt tcgagtttac gaatatcgac tttccctacc agggcggaga tgtcctggtt
     2761 gttctcatcg ccgggttctg ttttggcgat agcaatttgt tgcagaattg acggccagac
     2821 cttcactacc cggaacttag tgatatcgcc accaaattca tgcaggcgtt ttgccgccca
     2881 cggcgacatg atggtgccga gataacggcg aggaatgcca tactcttttt ccagaatctg
     2941 cgcatcttcc tgcggattga aaagacagaa cggatgatcg ttgaccgggc tacgctcacc
     3001 gttggcgctc aatacataaa tcggtacgag ctgcattaat gatttcagtc gctcagcaag
     3061 cgatgattta cccccaccca caggccccag cagatacagg atttgtttct tctcttccag
     3121 cccctgagcc gcgtgtttca gataagagac aatctgttca atcgcgtctt ccatgccgta
     3181 aaactcttca aacgccggat aacgtgcaat gacccggtta gaaaagagtc gagaaagtct
     3241 gggttcctgg gctgtatcga ccatgacagg ctcaccaata gccatcaata gccgctcagc
     3301 cgcgttggca taagcactgc gatcttgccg acaagtggta agaaactcct gcagtgtgaa
     3361 ctcttcgtcc ttggcagctt catatcgctg gcgatagtga tcgaatatat tcatggtatg
     3421 ccgtcctttc gttttttagc acaggttaag agccgttcat atgaatagtt gaggctcccg
     3481 gaagaggtaa actgaacgac gtctcttgct aaaacacagc aactctcatg ccattttatt
     3541 gacgatgaga acaacgtgcc cggtgataca ccttcaaaat taagcgtaga tggcaaatgg
     3601 aaaacttgcc tgcacggctt tcttaatttc aatggcatat caacaactca tccataaatt
     3661 ttgcataatt aatgtaaata atagggtcgc cggtaacgct aaattcattt ggctgtaagc
     3721 gtggtgtcat ctgcgtcagg aaaattaaac agttactata aaaaatgaaa acgtaaaaag
     3781 gttgggtttc gatgtattga cgggtaaact ttgtcgcccg ctaaacattt gtttaaggaa
     3841 tgattaattg tgaccaaact caaacttctg gcacttggag tgcttatcgc aacgtctgca
     3901 ggcgtagcgc acgctgaagg taaattttcc ctgggcgcag gcgtaggtgt cgttgaacac
     3961 ccatataaag attacgatac cgatgtttac ccagtaccgg taatcaacta tgaaggcgat
     4021 aacttctggt tccgtggctt aggtggtggt tactacctgt ggaatgacgc aacggacaaa
     4081 ctttcaatta ccgcttactg gtcgccgctt tacttcaaag ctaaagacag tggcgatcac
     4141 caaatgcgtc acctggatga ccgtaagagc accatgatgg ctggtctgtc ttatgctcac
     4201 tttacccagt acggttacct gcgtaccact ctggctggcg ataccctgga taacagcaac
     4261 ggcatcgtct gggatatggc ctggttgtat cgttacacca acggtggcct gaccgtgact
     4321 ccgggtattg gtgtgcagtg gaacagcgaa aaccagaacg aatactatta tggcgtatcg
     4381 cgcaaagagt ccgctcgcag cggtctgcgt ggctataacc cgaacgacag ctggagccct
     4441 tacctggagc tgagcgccag ctacaacttc ctcggtgact ggagtgttta cggtaccgcg
     4501 cgctacaccc gtctgtctga tgaagttact gacagcccaa tggtggataa atcctggact
     4561 ggcctgattt ctaccgggat cacctacaaa ttctgataat gcatttttcc agggcatttt
     4621 gtgcataaaa acagggcgta atgccctgtt ttgcattacg gtggtgcaat acctgggagg
     4681 aagcaatgca acaaaaaatg attcaattta gtggcgatgt ctcactgcca gccatagggc
     4741 agggaacatg gtatatgggc gaagatgcca gtcagcgtaa aacagaagtt gctgcactac
     4801 gcgcgggcat tgaactcggt ttaaccctca ttgataccgc cgaaatgtat gccgatggcg
     4861 gtgccgaaaa agtggttggg gaagcattaa tcggtctgcg tgagaacgtc tttctcgtct
     4921 ctaaagtcta tccgtggaat gctggcgggc aaaaagcgat aaatgcatgc gaagccagtt
     4981 tacgccgtct caatactgat tatctcgatc tctacttatt acactggtct ggcagtttcg
     5041 ctctcgaaga gactgtcgca gcgatggaaa aattgatcgc ccagggaaaa atccgccgct
     5101 ggggcgtttc taaccttgat tatgccgata tgcaggaact ctggcagctg ccggggggaa
     5161 atcagtgtgc cactaatcag gtgctttacc atctcggttc acgagggatt gagtacgatc
     5221 tacttccctg gtgccagaaa cagcagatgc cggtgatggc ttacagtcca ttagcccagg
     5281 ccgggcggtt gcgcaatgga ctgttaaaaa atgcggtagt caacgaaatt gcacatgctc
     5341 acaatatcag tgcggcacaa gtattgttgg cgtgggtaat cagccatcag ggtgtgatgg
     5401 cgattccaaa agcggcaact gtcgcacatg tccaacaaaa tgcggctgtg cttgaggttg
     5461 aactttcgtc agcggaatta gttatgctgg ataaagcgta tccggcacca aaaggaaaaa
     5521 ctgcgctgga tatggtgtga tgttgattcg tgccggatgc atcgcacccg gcacacaata
     5581 ttaacgcaaa ttaacgttta gcaacgcgaa tggattgcgc cagatgtgca ggtttctctt
     5641 tggtgacttt ttgcgtttct gaagcgtaag ccgtttctac acaaacaaag gtcttgtagc
     5701 catcatccgg catatcgccc atgctaactg aaagcgccgg tcctgggttc cagcctacaa
     5761 cgttcagatg atgctggtgg cctacggcga taatacgatt cagcgcttcg tcattaatta
     5821 cgctgcaatc ttgcggattc agatacacgc gatcggtacg gtcagggaag gtctgaatac
     5881 cgtcggtcag tacatcttct ttcgcgtcat ttactttatc aatgaagcga tcgcctagtc
     5941 cactgacgct taccttagcg atatcaccca cgttaaagta ggtatgcaga gcagaggtag
     6001 tttcaaattc gccgtgtgct tcaagatcga tttcacaggt tttacccacg tggaaatgcg
     6061 ctaacagcgt aaagtcgtgc ggccagaatt ttttcgtctc ttcgctttgt gtcaattcaa
     6121 aagtcagcgc tacaccatca gcatcttcac gatgtgattt cagcgtccac ggcaggttgc
     6181 gggcaaaacc gtgcgcaggc agaccttgtt gtgcagccgg accaaaccac ggccagcaaa
     6241 ccggtacgcc accgcgaata gcgacgccat ttttgaacgg tgtgttattg ctcaaccaca
     6301 gaacttcttc ttcacctgca ggtttccatg agagaaggtg tgcgccctgt aatgcaaaag
     6361 aggcttttac ctggggatga tcgaccacaa tgaggtccag ttcatccagt ttacgacggg
     6421 agaggacagg ggagatttgt tcgatgaccg gaagggcaaa aattttctta atcatgacgc
     6481 agtcctttaa cttcatttta tcaggtaaaa aaaagagcga ccgaagtcgc tctttttaga
     6541 tcacagtgtc atctcaactt atttggagat gtgagcgatc aggtccagaa ctttgttgga
     6601 gtaaccggtt tcgttgtcgt accaggatac cagtttcacg aagttgtcgt tcagagcgat
     6661 accagcttta gcatcgaaca cggaagtgca aacttcgccg ttgaaatcgg tagatactac
     6721 gtcatcttcg gtgtagccca gaacgccttt catttcgcct tcagcagcag ctttaacggc
     6781 agctttgatc tgctcgtaag ttgcagcttt ttccagacga acggtcaggt caactacgga
     6841 tacgttcggg gtcggaacgc ggaacgccat accagtcagt ttgccattca gttctggcag
     6901 tactttacct acagctttag cagcaccggt agaagacggg atgatgttct gggaagcgcc
     6961 gcggccgccg cgccagtctt tgtgagacgg gccatcaacg gttttctgag tagcggtagt
     7021 agcgtgaacg gtggtcatca gaccttcgat gatgccgaag ttatcgttga taactttagc
     7081 cagcggagcc aggcagttgg tggtgcagga agcgttggaa acgatgtcct ggccagcata
     7141 tttgtcgaag ttagcgcctt taacgaacat cggagtgttg tctttagacg gaccagtcat
     7201 aaccactttc ttcgcaccag cggtgatgtg tttacgagca gtttcgtcag tcaggaacag
     7261 accagttgct tcagcgacaa cgtcaacacc aacttcgtcc catttcaggt tagccggatc
     7321 acgttcagcg gtaacacgga tttttttacc gttaacgatc agatgaccgt ctttcacttc
     7381 aacggtaccg tcgaaacggc cgtgagtgga gtcatatttc agcatgtatg ccatgtaatc
     7441 agcgtctaac aggtcgttga ttgcaacgat ctcgatgtca gaacgtttct gagcagcacg
     7501 gaaaacaatg cgaccgatac ggccaaaacc gttgatacct actttgatag tcatatattc
     7561 caccagctat ttgttagtga ataaaaggtt gcctgtaaaa ttacaaaaac cttacgcagc
     7621 gtcaagcgga atcgtgtcaa tcattgcgac aaatcaatcc tgtgcctaag cattacgcga
     7681 ctgattcgcc tcactcttcc tttgggcttg agaccacatg gggtcggcgc cccgaatttt
     7741 aaagggcaat tacgataaaa atgtgatttt catcacgatt taggtgcagc catttcgtcg
     7801 agatcaagtt taggacaaaa ttttgccagt tggtgttaat gttttgttag aatcagtcac
     7861 gtaatgtgag cacgattaaa gtgagatgtg agcaaatggc taataaacct tcggcagaag
     7921 aactgaaaaa aaatttgtcc gagatgcagt tctacgtgac gcagaatcat gggacagaac
     7981 cgccatttac gggtcgttta ctgcataaca agcgtgacgg cgtatatcac tgtttgatct
     8041 gcgatgcccc gctgtttcat tcccaaacca agtatgattc cggctgtggc tggcccagtt
     8101 tctacgaacc ggtgagtgaa gaatctattc gttatatcaa agacttgtca catggaatgc
     8161 agcgcataga aattcgttgc ggtaactgcg atgcccatct gggccatgtc ttccctgacg
     8221 ggccgcagcc aacgggcgaa cgttattgtg ttaactctgc ttctttacgc ttcactgatg
     8281 gcgaaaacgg cgaagaaatc aacggttgaa aaaacgattc agcaacttat tccacaggag
     8341 cggaaaaatt atgaatcttg atgacattat caacagcatg acgcctgaag tataccagcg
     8401 tttgtcgacc gccgttgaac tggggaaatg gcctgatggc gttacgttga ccgaggaaca
     8461 aaaagagaac tgcctgcaac tggtgatgct gtggcaagcc cgccataata ccgaagcaca
     8521 gcatatgaca attgacacaa atggtcagat ggtcatgaag agcaaacagc agttaaaaga
     8581 agattttggt atctcagcaa agcctattgc catgtttaag taagtggttt atcccctttt
     8641 tgccgcggtt ataaagggga tagccaacaa tgaccccacg actcatctca attatctcgc
     8701 cccgacctgg ctaagtccca gtccgatctc ttgcagtcgc gttcacacct gaatggcttc
     8761 tcctgaactt aagtctcagg cctatttggc cgggaatccc tctcgaattt gctctttttg
     8821 cgtcccgcct tccatcaacc tgtaacagcc ccagccattt tctgcgtaat tgtgatccaa
     8881 gttgtaaaac gcagatcatt atctgtgttt tacagatttg atgttgacct caaaaatcat
     8941 gtgggtaagt atgatgacca tctaagcaaa acaaaacaca gaggatgacg caatgaaagc
     9001 actggctcgg tttggcaagg cctttggcgg ctacaagatg attgatgtcc cacaacccat
     9061 ttgtggcccg gaagatgtcg tgattgaaat taaagccgcg gcaatctgcg gcgcagacat
     9121 gaagcactac aatgtcgata gcggttcgga tgagtttaac tctatccgcg gccatgagtt
     9181 cgcaggttgt attgcgcagg ttggtgaaaa agtcaaagac tggaaagtgg ggcaacgcgt
     9241 cgtgtcggat aacagcggtc acgtttgcgg cgtttgcccg gcctgtgaac aaggtgattt
     9301 tctgtgttgt acagaaaaag taaaccttgg tctggataac aatacctggg gcggtggttt
     9361 ttccaaatat tgtcttgttc ctggtgaaat tctcaaaatt catcgtcatg cgttgtggga
     9421 aatccctgat ggtgttgatt atgaggacgc agccgtactt gaccctatct gtaatgccta
     9481 taaatccatc gcgcagcaat cgaaattcct tccaggtcag gatgtggtcg tcatcggcac
     9541 tggcccactc gggctgttct ccgtacaaat ggcgcggatt atgggggcgg taaatatcgt
     9601 cgtcgttggt ctgcaagaag atgtagcggt ccgcttcccg gttgcaaaag aactgggtgc
     9661 gacggcagta gtaaatggtt ctaccgaaga tgtggtggca cgctgccagc aaatttgtgg
     9721 caaagacaat ctggggctgg tgattgaatg ctccggtgcc aatatcgcat tgaaacaagc
     9781 catcgaaatg ctccgcccga atggggaagt ggtacgcgtt ggaatgggct tcaaacctct
     9841 tgatttctcg attaatgaca ttaccgcctg gaacaaaagc atcattgggc atatggccta
     9901 tgactccacc tcatggcgta acgctatcag gctattagcc agcggcgcta tcaaagtcaa
     9961 accgatgatc acgcatcgta tcggcctgtc gcaatggcgc gaagggtttg atgcgatggt
    10021 cgataaaact gcaatcaaag tgatcatgac ttacgacttt gatgaataac tactctcctg
    10081 aaggaacgtt atcgcatgga acagataaca aaaccgcatt gtggtgcacg gctggatcgc
    10141 ttaccggatt gccgctggca ttcgtcaatg tttgccatcg tcgcgtttgg cttgcttgtc
    10201 tgctggagta atgccgttgg tggcttgatc ctcgcgcagc tgaaagcgtt gggttggaca
    10261 gataattcca ccactgccac attctcagca atcacgaccg ccggaatgtt tctcggtgcg
    10321 ctggtgggcg gcatcatcgg tgacaaaacc ggtcgcagaa atgcgttcat cctctatgag
    10381 gccattcata ttgcttcgat ggttgtcggt gctttctcac cgaatatgga tttccttatt
    10441 gcctgccgtt ttgtgatggg cgttgggctg ggagctttac tggttacact gtttgctggt
    10501 ttcaccgaat atatgcccgg aagaaatcgc ggaacgtggt ccagtcgggt ttcctttatt
    10561 ggtaactggt catatccgct ctgttcattg attgcgatgg gactcacgcc gctgattagt
    10621 gcagagtgga actggcgggt acaactgctt atccctgcaa tattgtcact tatcgctacg
    10681 gcgctggcct ggagctactt tcctgaatcc ccgcgctggc tggaatcgcg cggacgttat
    10741 caggaagccg agaaagtcat gcggagtata gaagaaggcg tcatacgcca gacgggtaaa
    10801 cctttgccgc ctgtggttat tgctgatgac ggtaaagcgc cacaagcggt gccatattca
    10861 gccttactga caggagtatt actgaaacgc gtgatattag gctcttgtgt gctgattgcc
    10921 atgaacgttg tgcagtacac actaattaac tggttgccaa cgatattcat gacccaggga
    10981 attaatttaa aagactcgat tgttttaaat accatgagta tgtttggcgc gccatttggt
    11041 atttttattg ccatgctggt gatggataaa attccgcgta aaacaatggg tgtggggcta
    11101 ttaatcctga ttgcggtgct cggatatatc tattcactgc aaaccagtat gttgctcata
    11161 acgctgattg gtttcttcct gattactttc gtctatatgt acgtttgcta tgcctcggca
    11221 gtgtatgtcc ctgagatctg gccgacagag gccaaactcc gtggctccgg tctggcgaat
    11281 gcggtagggc gaatcagtgg tattgccgcg ccttatgccg ttgcagtgct gctcagtagt
    11341 tatggcgtaa cgggagtctt tattcttctg ggggctgttt caattattgt cgcaattgct
    11401 atcgccacca ttggaattga aaccaaaggt gtctccgttg aaagtttaag tattgatgca
    11461 gtagccaata aataatactg gtggattcaa aatgaaaaat tcaaaagcaa tattgcaggt
    11521 gccgggcaca atgaaaatta tttcagcaga aatacctgtg cctaaagaag atgaagtttt
    11581 gattaaagtg gaatatgtcg gtatttgtgg ttcagatgta catggttttg aatcaggccc
    11641 gtttattccg cctaaagacc caaatcaaga aattggcctg ggtcatgaat gcgccgggac
    11701 ggttgtggct gtgggaagcc gcgtgcgcaa atttaaaccg ggggatcggg taaatatcga
    11761 acctggcgtt ccttgcggtc actgtcgtta ctgtctggaa ggcaaatata acatttgccc
    11821 ggacgttgat tttatggcga cacaacccaa ctaccgcggc gcattaacgc actatctgtg
    11881 tcatccggag agctttactt acaaactgcc agacaatatg gacacgatgg aaggggcgct
    11941 ggtggagcct gccgcagtcg ggatgcatgc cgcgatgctg gcagatgtta aaccgggtaa
    12001 gaagataatt attctgggag cgggttgtat tggtttgatg acgttgcaag cgtgcaaatg
    12061 cctgggagca acggaaattg ccgtcgttga tgtgctggaa aaacgtctga caatggcgga
    12121 acagctcggc gcgacagtgg ttattaacgg cgtaaaagaa gacactattg cacgctgtca
    12181 gcaatttacc gaagacatgg gcgcagatat tgtttttgaa acagcgggtt ctgcggtcac
    12241 cgttaaacag gcaccttatc tggtcatgcg cggcggtaaa attatgattg ttggtactgt
    12301 acccggcgat tcggcaatca atttcctcaa aatcaatcgc gaagtcacta tccagacggt
    12361 attccgctat gccaatcgtt atccggtcac gattgaagct atttcttcag ggcgattcga
    12421 tgtgaaatcg atggtgacgc atatttacga ttatcgggat gtacaacagg catttgaaga
    12481 gtcagttaac aacaaacgcg acattattaa aggcgttatt aaaattagcg attaattaat
    12541 acgggagaac agaacatgct cgcagatatc agatattggg aaaacgatgc cacaaataaa
    12601 cattatgcaa ttgcccattt taacgtatgg aatgcagaaa tgttgatggg cgttatcgac
    12661 gcagccgaag aagcgaaatc accggttatt atttcttttg gcacaggttt tgtcggcaac
    12721 acctcatttg aagatttctc tcacatgatg gtatcaatgg cacaaaaagc aacggtgccg
    12781 gtaataactc attgggatca tggtcggagt atggagatta ttcataacgc ctggactcat
    12841 ggtatgaatt cattaatgcg tgatgcttct gcatttgatt tcgaagaaaa tattcgttta
    12901 accaaagagg ctgttgattt cttccatccg ctgggtattc cggtagaggc ggaattaggg
    12961 catgttggta atgaaaccgt ttatgaggaa gcgctggcgg gctatcacta taccgatcct
    13021 gaccaggcgg ctgaatttgt tgaacgtacg ggctgtgact cattggctgt cgccatcggc
    13081 aaccagcatg gggtttatac gtcagagcca caattgaact ttgaggtcgt caaacgcgta
    13141 cgcgatgctg tttctgttcc gctggttttg catggcgcat cagggatcag tgatgccgac
    13201 attaaaactg caatttcgtt gggtattgcg aaaatcaaca ttcatacgga gctctgtcag
    13261 gccgcaatgg tagcagtgaa agaaaaccag gatcagcctt tcctgcattt agaacgtgaa
    13321 gtgcgtaaag ctgtaaaaga acgggcattg gataaaatta aactgttcgg ttctgatggc
    13381 aaagcggaat aataatatgg ataatctcga cgttatttgt ataggtgccg ctattgttga
    13441 tattccattg caaccggtca gtaaaaatat ctttgatgtg gattcttatc ctcttgaaag
    13501 aatcgcaatg accaccgggg gggatgcaat aaatgaagca acaattattt ctcgtctggg
    13561 ccatcgcaca gcattaatga gtcgtattgg taaagatgcc gcagggcaat ttattctcga
    13621 ccattgccgc aaagagaata ttgatattca aagcctgaag caggacgtca gcatagatac
    13681 ctctattaac gttggactgg tgacggagga tggcgagcgg acctttgtca caaaccgtaa
    13741 cggcagtctg tggaaattga atattgacga tgttgatttt gcgcggtttt ctcaggcgaa
    13801 attattatca ctggccagta ttttcaacag tcctctattg gatggtagag cgctaacaga
    13861 aatttttacg caagctaaag cccggcagat gattatctgt gccgatatga tcaaaccgcg
    13921 gttgaatgaa acgctgaatg atatttgcga agcattgagt tatgtcgatt atctgtttcc
    13981 taattttgcc gaggcaaaat tactcaccgg gaaagaggca ctggatgaaa ttgctgactg
    14041 ctttcttgcg tgcggcgtaa aaacggtggt gattaaaacg ggtaaagacg gctgctttat
    14101 caagcgtggt gacatgacga tgaaggtgcc ggcggtcgca ggaataaccg ccatcgacac
    14161 cattggtgcg ggcgataact ttgcttcagg ttttattgca gcactgttag aaggtaaaaa
    14221 tctgcgtgaa tgcgcacgct ttgccaatgc aacggcggct atctcggttc taagcatcgg
    14281 cgccaccact ggtgtaaaaa acagaaagct ggtcgaacaa ttgctggaag aatacgaagg
    14341 ataatgaagg caaatgaaaa agataccttt aggcacaacg gatataacgc tttcgcgaat
    14401 ggggttgggg acatgggcca ttggcggcgg tcctgcatgg aatggcgatc tcgatcgaca
    14461 aatatgcatt gatacgattc ttgaagccca tcgttgcggc attaacctga ttgatactgc
    14521 gccaggatat aactttggca atagtgaagt tatcgttggt caggcgttaa aaaaactgcc
    14581 ccgtgaacag gttgtagtag aaaccaaatg cggcattgtc tgggaacgaa aagggagttt
    14641 attcaacaaa gttggcgatc ggcagttgta taaaaacctt tctccggaat ctatccgcga
    14701 agaggtagaa gccagcttgc aacgtctggg tattgattac atcgatatct acatgacgca
    14761 ctggcagtcg gtgccgccat tttttacgcc gatagctgaa actgtcgcag tgcttaatgc
    14821 gttaaaggcc gaagggaaaa ttcgcgctat aggcgctgct aacgtcgatg ctgaccatat
    14881 ccgcgagtat ctgcaacatg gtgaactgga tattattcag gcgaaataca gtatcctcga
    14941 ccgggcaatg gaaaacgaac tgctgccgct atgtcgtgat aatggcattg tggtacaggt
    15001 ctactctccg ctagagcagg gattgttgac cgggaccatc acgcgtgatt acgttccggg
    15061 cggcgctcgg gcaaataaag tctggttcca gcgtgaaaac atgctgaaag tgattgatat
    15121 gcttgaacag tggcagccac tctgtgctcg ttatcagtgc acaattccca ctctggcact
    15181 ggcgtggata ttaaaacaga gtgatttaat ctccattctt agtggggcta ctgcgccgga
    15241 acaggtacgt gaaaatgtcg cggcactgaa tatcaactta tcggatgcag acgcaacatt
    15301 gatgagggaa atggcagagg ccctggagcg ttaaatactc aacacgattc acctctgttt
    15361 ctgtgaaaac tgtgatgaaa atctgtgatt cagataggtt attttgaaag taaacatcgg
    15421 ttatgcgata attgcgctaa tgtgatgtaa aaagatacag gggtgtaatc gtggcggcaa
    15481 aagacaggat tcaggcaatt aagcaaatgg ttgccaacga taaaaaggtg acagtctcaa
    15541 atttgagtgg gatttttcag gtaaccgaag aaaccattcg ccgcgatctt gagaagttgg
    15601 aagatgaagg ctttttgacc agaacctatg gtggtgctgt tttaaataca gcgatgttga
    15661 cggagaatat ccatttttat aagcgcgctt catcgtttta tgaagagaag cagcttattg
    15721 cacgcaaggc actacccttt atcgacaata aaaccaccat ggcagccgat tccagtagta
    15781 ccgtaatgga attgctcaaa ttgttacagg accgtagtga cctgacgttg ctaaccaact
    15841 cggcggaagc gattcatgtt ctggctcagt cagaaattaa agtcgtttca acgggtgggg
    15901 aactaaacaa aaatacactt tcactgcaag gaagaattac taaagagatc atcagccgct
    15961 accatgtcga tatcatggta atgagttgta aaggtcttga tattaacagc ggcgcgctgg
    16021 actctaacga agcggaagct gaaatcaaaa agacaatgat ccgtcaggcg acagaagttg
    16081 cgttattggt tgatcactct aagtttgatc gcaaagcttt tgtccagtta gctgacttta
    16141 gtcatattaa ttacataata actgataaat caccgggtgc agagtggata gcattttgca
    16201 aagacaataa tatccagctg gtgtggtaat ttaagcacac attgtcaata ttatttgttc
    16261 ctgcgcctgg aaataaaatg atgataaaga tgcggcaagt gaatttgccg caattgctac
    16321 cttttttagc aaatccgagg cacccaatgg aacaatatga tcaaattggc gcaagactgg
    16381 accgcttgcc tttggcccgg tttcattatc gtatatttgg tataataagc tttagtctgt
    16441 tattaacggg gtttttgagt tactccggta atgtcgtctt agcaaagctg gtaagcaatg
    16501 gatggtcaaa taatttcctc aatgccgcct ttacctcggc attaatgttt ggttatttca
    16561 tcggctctct taccggtggg tttattggtg actactttgg gcggcgcagg gcgtttcgca
    16621 taaatcttct catcgtcggt attgcggcaa caggggccgc ttttgtccct gatatgcact
    16681 ggctcatctt ctttcgcttc ctgatgggaa caggaatggg ggcgctgatt atggttggct
    16741 atgcctcatt tacggagttt atccccgcga cggtgcgtgg aaaatggtcc gcgcggctct
    16801 catttgttgg taactggtcg cccatgctgt ctgcggcgat aggcgtggtg gttatcgctt
    16861 tttttagttg gcggataatg tttctgctgg gcggtattgg catactgtta gcctggtttc
    16921 tctcaggtaa atactttatt gagtcgccac gatggctggc agggaaaggg caaatcgcag
    16981 gtgcagaaag ccaacttcgt gaagtagagc agcaaattga aagagagaag agtattcgtt
    17041 tacccccgct tactttgaac cagagcaaca gcaaggttaa ggtaatcaag ggtaccttct
    17101 ggctgttgtt taaaggggaa atgttacgac gtacattagt cgcgattact gttttaattg
    17161 caatgaacat ttcgctttat accatcaccg tatggatacc gaccatattt gttaactccg
    17221 gcattgatgt tgataaatca atattaatga ccgctgttat tatgattggc gctccggtag
    17281 ggatatttat tgcggcatta attattgatc attttcctcg tcgattattt ggctctgcct
    17341 tacttattat tattgctgtg ttaggctata tctattcaat tcagactaca gagtgggcga
    17401 ttttaatcta tggcctggtg atgatcttct ttttatacat gtatgtgtgc ttcgcgtcgg
    17461 cggtttatat cccggaactt tggccaacgc atttacgcct gcgtggttcg ggtttcgtta
    17521 atgccgtcgg acggatcgtc gcagtcttca cgccctatgg cgttgcggca ttattaacac
    17581 attatgggtc gatcacggtg tttatggtgc ttggtgtcat gttagtgctc tgtgcgctgg
    17641 ttctctccat ttttggcatc gaaacgcgga aggtgtcgct ggaagagatt tctgaggtga
    17701 attaaatctt tcgttgatgg cgtcaattac cagatgtatc actgatgtgt tttatctggc
    17761 ctacaatgcc agtacagaat tgtaggccag cgtaaaatta ctcctgcgtt tcttcccagt
    17821 ctgccagcgt atatagcgtt gccccagctg ctgacatctc cataaacgcg tgcgcactgt
    17881 cctgtggctg gatatttacg ccacgacaac catcggtaat cacgttcacc ttatagccta
    17941 actgtaacgc gtccagcacg gtaaacttaa cgcaatagtc agtagccaga cccataacga
    18001 tcaattcatt gattacatga gcgcgcaacc agtcatcgag cgcagttttc tgccgacggc
    18061 cgttatcaaa aaaggcactg taactgtcaa ctaaaggatt ttcgccttta tggaacaccg
    18121 ctgcgatcgc tttttgcttc agtaacggat gtaattgtgc gccttcactg ttctgcacac
    18181 agtgatcggg ccagaaggtt tgtggcaaac cgtcgagttg gcccggcgta taaggctcta
    18241 caccgtgctg actggcaaaa ctgccgtgat tcgccgggtg ccagtcctga ctggcgataa
    18301 ccgcttcacc gcgcgactgg caccagtcaa tcaggcggtt agcgacatcc accgtactgt
    18361 caccttccgg cacggcgagc gcgccaccag cacagaaatc attttgtaaa tcgaccagta
    18421 acagggcgcg agggggcatt gcagtctcct taatcatccg gcgtcagttc gccacgcagg
    18481 ttttggctca tggccttgcg aatggtttca gtatcaagtt cctggctaag caggtaatgc
    18541 agtttggtta gcgtggcttc tacagtcata tctgcaccgc caataacgcc ggcatgggcg
    18601 agggcgttac cagtggcata accacccatg ttcactttac cggacataca ttgtgtcagg
    18661 ttgaccacca caataccgcg atcgctggct tcttgtaatt cctgcaggaa ggctttgttt
    18721 tgtggcgcat tacccacgcc atatgagcgc agaatcaatg ctttcacagg ttggcgcaga
    18781 aaattgcgca cgacgtcagc agaaatcccc ggataaatcg tcactacgcc aattggttgc
    18841 ggcgtgattg gatgaacgat caattcccct tcaccgtgcg gggcgggtgg cgtattcaaa
    18901 cgacgaatat ggatacctgc ttccagtaac ggaggaaggt ttggagaggc aaacgcatca
    18961 aaaccatcgg catgggcttt ggtggttcgg ttgccacgat acagtcgatt attaaaaaag
    19021 agagttactt cgttgatagg ataattcgcc gcaacgtaca acgcattcag tagattaatt
    19081 tgtccgtcag agcgtaactc agccagcggg atttgtgacc ctgtcacaat gaccggttta
    19141 ccgagattct cgagcatgaa cgacagtgca gaggcggtat acgccatcgt gtctgtaccg
    19201 tgcagaatga caaaaccatc ataatcgtca tagtgcgctt taatatcttc agcaatatgc
    19261 tgccagtctt ctggcgtcat atcagaagaa tccatcaatg gcgtatattc atgaatggtg
    19321 aaatctggca tctccgggcg atggaattcc ggcatcagcg ccagttggcg ctgtagatga
    19381 cctgacaccg gaatataacc ctgctcggaa cgctgcatcc cgatggtccc gcccgtgtag
    19441 gcgacgtaaa ttgatttctt ttgcatgata ttgatatgaa tgatatcgaa agagcaaaag
    19501 tatatacgtg aggcaggggg agcgcacctg atactatgag gataaggaaa tatgtggcga
    19561 gacagcggga agaggattcg tcgacgtaaa aagtaatgtg gccgacgatc tgtcggccac
    19621 ttagtacaag acttaacgca cgttggcgca ggtcaggcaa aacgcataac ggttttgtgg
    19681 gtcgttaaac gcggccagct tgtcgctttc acttttaacg gtagaggcta ccgaggccag
    19741 cggtgcaggt aacatggcct ggaacgcatc tggcaacatt gcccggacag aaccagacat
    19801 gttgtccatc actttgtcga agaaggtcgg ttcatcaacg tagtattcca gatgccactg
    19861 tttcactttt gccagctctg ctgctttggc gaccgcatca tcgaaatccc cgagactatc
    19921 gaccagcccg ttagcttttg catcctggcc ggtccagacg tggccctggg cgattttatc
    19981 aatctgctcc ggcgttgaat gacgcgcatc agcaaccagc gtgataaagc gtttatagcc
    20041 attctcaatg cttaactgca tcatctgctg cgcttccggc ggcagtgccc tggtgataga
    20101 aacatccgcc agcggtgaag ttgagacacc atcagtatga acaccaatcg aatccagact
    20161 attttctacg gtggtgatca cgccgaagat accgatagaa ccggtcaggg tgctggggtt
    20221 agccacaatg taattagctg gtgtggaaat ccagtaacca ccagatgccg ccatgccacc
    20281 catcgataca accacaggct tacccgctgc ccgggctgct gccagttcag cgcgaatcac
    20341 ttcagacgcg gtaacgctgc cgccgggact attaacacgc agaacaatcg ctttcacttt
    20401 cgggtcaagg cgagcgtcgc ggatttgtgc cgcagtggta tcaccgccaa cattcccctg
    20461 agtttcctcg ccatccataa ttgcgccatt agcgaagacg acaccgatac tgtcaccggt
    20521 atctgccggc gttttcaatg cgtaatcgta ataactgatg gcgcgataat ttttatcagt
    20581 tttactccag ccgaactctt tggtcagagt tttttcgatt tcggcactcg atgccagtgc
    20641 atcgaccagc ttgttttcca gcgcatattt cgcggtatcg ccaccggttt tggttaaacc
    20701 ctcaagcaac ccttgcgcgc cagggaatac ctgctgagca gggatctggc ggttagcagc
    20761 aacagtattc agatagtttt tccacagctc accaatccag cggctgtcag cttcgcgggc
    20821 tgccgacgac atatcatcac gaataaacgg ttcaacggca gatttatacg tacccacgcg
    20881 gaacacatgg gtggaaactt tcagcttatc cagtaacgat ttgtagtaca gaccgttggt
    20941 ggcaaagccg tgcagatcaa ccacgccttg cggagacagc caaattttat tggcgaaact
    21001 ggcgagataa tattgcccct ggctgtagtt ctcgccaacg gcataaaccg gtttcccgct
    21061 gtcacgaaac tctttcagag ctttgccgat gtactgcata gacggctggt cgccgcctgc
    21121 gaagtttttc agatccatca caataccggt gatattgcgg tcgtccttcg cctggcgaat
    21181 ggtgttgacg atatcaaaca gtgagttttc ctgcagacga tcggaactgg caccaagcag
    21241 ctggcggctt aatttactaa accgctgaga actgtcgggt ttatcgacga tcacaccaga
    21301 aatgtccagc agcagtgcgc cacgactggc cgtttctttc gaatccccac cactgacctg
    21361 catccagatc cccacaccaa ccagcacgag aaaaataaag aacaggttaa gtaccatttc
    21421 acggacgaaa ttcagcagac gccacgtcca tttaaaaaat ccggcaataa atcgccaaag
    21481 ggttcgcatg tattctccca acttaaggtc acacctgttt caggcgcaat agatgtggtt
    21541 atcgtaatga cccaaccggc acttgtcagc aggaattgcg gactgggctg taacaaaatc
    21601 cactctcgtg ttaattttgt gaataaatat catgacagga gttaatcaaa tggatgcact
    21661 cgaactattg atcaatcgcc gtagcgcctc ccgcctggct gaacccgcgc caacgggtga
    21721 acaactgcaa aacatcctgc gtgcgggtat gcgtgcgccg gaccataagt ccatgcaacc
    21781 gtggcatttc tttgtgattg aaggggaagg gcgcgagcgt ttcagcgctg tactggagca
    21841 gggggcgatt gctgccggta gtgataacaa agcaatcgac aaagcgcgta atgcgccgtt
    21901 ccgcgcaccg ctcatcataa cggtggtggc gaaatgcgaa gaaaatcata aagtcccgcg
    21961 ctgggaacag gaaatgtctg cgggatgcgc ggtcatggcg atgcaaatgg cagcagttgc
    22021 ccaggggttt ggcggcatct ggcgcagtgg tgcattaact gaaagtccgg tagtgcgtga
    22081 agcattcggt tgccgtgagc aggataaaat tgtcggtttt ctctacctcg gtacgccgca
    22141 gttgaaagca tctacgtcga taaacgtccc ggacccgacg ccgtttgtga cttatttctg
    22201 ataattgtcg cgaaactgtc tggattgtga gcaaaggcgc aggaattcag acactctcac
    22261 ttatcacttc atggaatgag ggttaccata cccggattgc attgacagga gatgtccatg
    22321 agcgagaact cgattcgttt gacccaatac agccacggcg ctggttgcgg ctgtaaaatt
    22381 tccccaaaag tgttggaaac catcctgcac agtgagcagg cgaagtttgt tgatccgaat
    22441 ttgcttgtgg gtaatgaaac ccgcgacgat gcggcggtat acgatctggg caatggcacc
    22501 agcgttatca gtaccaccga cttctttatg ccgatcgttg ataatccttt cgattttggc
    22561 cgcattgcgg cgactaacgc catcagcgat atctttgcta tgggcggcaa accgattatg
    22621 gcgattgcga tcctcggctg gccgattaac aaactttccc cggaaattgc ccgcgaagtg
    22681 accgaaggtg gacgctatgc atgccgtcag gcggggattg cgctggctgg cggtcactcc
    22741 atcgatgcgc cggagccgat ttttggtctg gcggtaacgg ggatcgtacc gaccgagcgg
    22801 gtgaagaaaa acagtaccgc acaagccgga tgcaaactgt tcctgacgaa accgctgggg
    22861 attggcgttc ttaccacggc tgagaaaaaa tcactgctga aaccagaaca tcagggactg
    22921 gcgacggaag tgatgtgccg gatgaacatc gcaggcgcgt cctttgccaa catcgaaggc
    22981 gtaaaagcga tgaccgacgt tacgggtttt ggtttactgg ggcatttaag cgaaatgtgt
    23041 cagggcgcag gtgtgcaggc gcgcgtcgac tatgacgcga tcccgaaatt gccgggggtt
    23101 gaagagtaca ttaagttggg cgcagtgcct ggcggcaccg aacgtaactt tgccagctac
    23161 ggtcatctga tgggtgaaat gccgcgtgaa gtgcgcgatc tgctgtgcga tccgcaaact
    23221 tctggcggtt tgctgctggc ggtcatgccg gaagcagaaa atgaggtcaa agctacagcc
    23281 gccgagtttg gcattgaact gacggcgatt ggcgaactgg tgccagcgcg cggcggtcgt
    23341 gccatggttg agatccgtta attcaatgcg gttgtttatt gccgaaaaac cgagtctggc
    23401 gcgcgccatt gctgatgtcc tgcctaaacc gcaccggaaa ggcgatggct ttatcgagtg
    23461 cggtaatggt caggtggtga cctggtgtat cggtcacctg cttgagcagg cgcagccaga
    23521 cgcctacgac agccgctatg cgcgctggaa tcttgcggat ttgccaattg tcccggaaaa
    23581 gtggcaatta cagccccgac cctccgtgac taaacaactt aatgtcatca aacgcttcct
    23641 gcatgaagcc agcgaaatcg ttcacgccgg agacccggat cgcgaagggc aactgctggt
    23701 ggatgaagtg ctggactacc tgcaactggc accggaaaag cgccagcagg tacagcgttg
    23761 tttgataaac gacctgaacc cgcaggcggt tgagcgggcg atcgatcgtc ttcgctccaa
    23821 tagcgagttt gtaccgctgt gcgtttctgc actggcgcgg gcgcgggctg actggctgta
    23881 cggcattaat atgacccgtg cgtataccat tctcggccgc aatgccggtt atcagggcgt
    23941 gctttccgtg ggacgcgtgc agacgcccgt actcggactg gtggtgcgcc gcgacgaaga
    24001 gattgaaaac ttcgtggcga aagacttctt tgaagtcaaa gcacatatcg tgacacctgc
    24061 cgatgagcgg tttaccgcta tctggcaacc gagcgaagcg tgtgaaccgt accaggatga
    24121 agaagggcgc ttgttacatc gtccactggc ggagcatgtg gttaaccgca ttagtggtca
    24181 accagctatt gtcaccagct ataacgataa acgggaatca gaatccgcgc cgctgccttt
    24241 ttcgctttcg gcgttgcaga ttgaagcggc taaacgtttt ggtctgagcg cgcagaacgt
    24301 gcttgatatc tgccagaagc tgtacgaaac gcacaagcta atcacttatc cgcgttccga
    24361 ttgccgctat ttgccagaag aacattttgc cggacgccac gcggtgatga atgctatcag
    24421 cgttcatgca ccggatctgt tgccgcagcc agtggtagat ccagatatac gcaaccgctg
    24481 ttgggatgac aaaaaggtcg atgcgcacca cgccatcatt ccgaccgcac ggagttctgc
    24541 gatcaacctg acggagaacg aagcgaaggt ctataacctg attgcccgtc agtacttgat
    24601 gcagttctgc ccggatgcgg tgttccgcaa gtgtgttatc gaactggata ttgccaaagg
    24661 caaatttgtc gctaaagccc gttttcttgc tgaagcaggc tggcgcacgc tgttaggcag
    24721 caaagagcgc gatgaagaaa acgacggcac gccgctgcct gtggtggcga aaggcgatga
    24781 attgttgtgc gaaaaaggcg aagtggtaga gcggcaaacc cagccgccac gccattttac
    24841 cgatgcaaca ctgctttcgg cgatgaccgg gatcgcgcgc tttgtgcagg acaaagatct
    24901 gaaaaagatc cttcgtgcga ccgatggtct ggggacagaa gcaacgcgtg ccgggattat
    24961 tgaattgttg ttcaagcggg gtttcctgac caaaaaaggg cgctatatcc actccaccga
    25021 cgccggaaaa gcgctattcc attcgctgcc agaaatggcg acgcgaccgg acatgaccgc
    25081 gcactgggaa tcggtgctga cgcaaatcag cgaaaagcag tgtcgctatc aggactttat
    25141 gcagccgctg gtggggacgc tttaccaact gattgatcag gctcgtagca ctccggtcag
    25201 acggttcaga ggaattgtgg catcgggtgg gggggctgtt aaaaaagcac ccaataagcg
    25261 tgtgaaaaaa gcggcaaagc ccaaaacaga taatacggat tctgctaatt aattcgtttt
    25321 ccactcttaa ataaagccaa tggtagatat tctgccattg gctttattgg ttatactcgt
    25381 catgcttcaa gttgcatgtg ctgcgtctgc gttcgttcag cccagtcact tacttatgta
    25441 agctcctcgg gattcactta ctt
//
LOCUS       DAZFJL010000066        25336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFJL010000066 DAZFJL010000000
VERSION     DAZFJL010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.066"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..227
                     /locus_tag="ACHJ8C_002589"
     CDS             <1..227
                     /locus_tag="ACHJ8C_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5552430.1"
                     /translation="SHNSHALDYIIKKAFTPRKTKTSTATHNVYSYNKEESTLNFQKI
                     GEAIFFGTDLVIKLKPNISASGELFICQKD"
     gene            231..1226
                     /locus_tag="ACHJ8C_002590"
     CDS             231..1226
                     /locus_tag="ACHJ8C_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFO5552431.1"
                     /translation="MNIVSEFLEKVTTIVESTAATNAAKVAQAISPTFFAAIALYVIY
                     LIYEITYAQRDILMNEVTKNIGAFALVGAFTYSAPYYSQFVIPFVMHAGSDLSAAVTG
                     GSGTATSVDNLWNMLSVTLNDFKNNELDTLEWYSFTDQLYIYLIWGVGYVGGLLLIYY
                     TTVFLTLSTFMVGILLSAGILFICFSLFASTRNMFSAWVGSCLNYILLNLFYSISFSF
                     VISFVEQTVPSSGNITLTTVVYFFMVTVISIFLVEQVGTLCSTLTGGVGINGLTSAAN
                     GFGGKVASGFMRASGLRSFTNGFASKMPNPFFNAGSRSASALVQGLNRGSKLKAG"
     gene            1232..1873
                     /locus_tag="ACHJ8C_002591"
     CDS             1232..1873
                     /locus_tag="ACHJ8C_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFO5552432.1"
                     /translation="MKDKVLSFLFAGIITITSPSIFASGIPVVDVAAIAKTVEEGLNR
                     AAEAARQLEQLKQQYEQTIRYAEEQKKRLEGFTDFSNGFDSASSYMKDSLSTITNSAK
                     SDLSSLRSQYDLSSNVAETQQKYDAILAKIKFYENFNNEMQERAKRLTTLQKEFASAD
                     TPQKKADLSNQLNTEKLTMELQLKQYDIAERQLENERKAQYETYVRQKRRDLS"
     gene            1875..2129
                     /locus_tag="ACHJ8C_002592"
     CDS             1875..2129
                     /locus_tag="ACHJ8C_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HFO5552433.1"
                     /translation="MNKSRNAIIASSIIFASLMLVGCKEKIYSVEYYSNNISEATKTL
                     EDCKKGTITDQNCDNARAALQQKQDSEYKKKVSEMRRRLD"
     gene            complement(2262..3071)
                     /locus_tag="ACHJ8C_002593"
     CDS             complement(2262..3071)
                     /locus_tag="ACHJ8C_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5710 domain-containing protein"
                     /protein_id="HFO5552434.1"
                     /translation="MNEPLEAELHSLFSFDIYPGASSEQNTGVKLAMARFYLNVPFEE
                     KDLAKQKGAQWDQEQRKWFVPQGKNPIYFIRWIKELNEHDYNVFSQRFYIAESYQSCW
                     RCKKTTPVFGIFLPRWYKYRDVIWGVDPAEWEDCILDEWYETSSPKGMEYFDSKKNMI
                     YRWLTSRVWWTDLTKIEIISTSALSRINEYSKLYYPSHSKTAKMKYYANHCCHCNAMQ
                     GDFMMFNEPGGVFFPVTYEQAEKIRFHEVNETIFAKASYSLIPEAGGFIDL"
     gene            3119..3754
                     /locus_tag="ACHJ8C_002594"
     CDS             3119..3754
                     /locus_tag="ACHJ8C_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5552435.1"
                     /translation="MKSIILSMVAATALLSGCADSKTQPVPESPLTSNDHQITLKIDT
                     VRTDLPDSSPVCLNGMNIYESLSERHPGLFAKNQNVIFRSAFFDDGQAISSETIRKIM
                     QSTPCVESQTTSAGSWEPATELIPRQAISGDTTLAEKDRTAGFTMRIAPEIERGNFHL
                     LLTTTDPIRKQTVEQNILLKPDQPLIVSGFTGVNADSQSENRIVIITPHVR"
     gene            3821..4120
                     /locus_tag="ACHJ8C_002595"
     CDS             3821..4120
                     /locus_tag="ACHJ8C_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HFO5552436.1"
                     /translation="MKSIILSMVAASAIALSAPVYASDPCASVLCLYGKAIGSGGGSE
                     CKSAEKDFFNIIKKKKGSIRWSKTFDARKAFLNQCTTADPAAISKIMSKFGRSWG"
     gene            4123..5358
                     /locus_tag="ACHJ8C_002596"
     CDS             4123..5358
                     /locus_tag="ACHJ8C_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HFO5552437.1"
                     /translation="MKKLIIIPTTLLLNACSHAPEPAQAKGGWYELNTTIEQVRADTY
                     YNGVILQQPVSQPNSTVQYSNDNYLKKEAINVTPAPRIAETVKPRSEQSATAPKNQGS
                     NNSFLKPTTNSITSSGEIKTAAKGATKQHSEQPAFSPAKPISSNNHQAQTAQKTDQQT
                     KPRPEQPPLMSKSGFLKKNANNAVPATTLTGPSTPEKQIYFVRHDGKGSLQKALKEIV
                     PSDWTAEISLEVTKTFRRTISWQGNDQWPYVLDKMLRNYGLTVIKNTEKRHLLIDFQQ
                     QKGKTSQKAPVSPDKTPASPVRTPVSSDKLPQKPLTPVTVPQKQTEKLTWNLKKGSTL
                     RDGLTEWASSSPCGNSTWNLVWDTPVNYRIDAPLIFRGNFESALKDTLMLYLSADKPL
                     YAFRNIAQCVITIKDTQQE"
     gene            5364..5801
                     /gene="pilM"
                     /locus_tag="ACHJ8C_002597"
     CDS             5364..5801
                     /gene="pilM"
                     /locus_tag="ACHJ8C_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFO5552438.1"
                     /translation="MGWFFSLLLIFLSIGSYYLTDNNNQTIQRMKLLPAKQEAIEFVH
                     FANVINDYLYKYPDKRNSGGTLTSEQIGITPVYDIHHIIYGKRVYIWSADTEGLMSAL
                     QQQTKHSAMLGRVKNKKIVDNQGNDMGVPVPSSIPEEAIVLIN"
     gene            5920..6318
                     /locus_tag="ACHJ8C_002598"
     CDS             5920..6318
                     /locus_tag="ACHJ8C_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5552439.1"
                     /translation="MQDNDDVSELDIATREVLILRCNLIQLASEYIKINPLKNTRIYT
                     DLIHRSADVIKNIEQGNIQNKHNYLSQDVKHTLVLYDLYKSDFEQCPGAKSLIPVREK
                     IVSLKELLSGANELDSIVRRPRRKKRSSRI"
     gene            6339..6923
                     /locus_tag="ACHJ8C_002599"
     CDS             6339..6923
                     /locus_tag="ACHJ8C_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO5552440.1"
                     /translation="MQLSAAVLAALISQCAPDVSPDTMNALIMTESGANPYVIANVSD
                     GTSKYFKDEKGAIEYAEKLTAENKRFSAGLTQIYSKNFPSLNLTNKTVFDPCTNIKAG
                     AAVLTDNYLRQKEGSSNQKILRALSLYYSGNESTGFIKEKKFNNTSYLERVIRNANNY
                     IVPSIREKTDESEIKPPNDEEKTSPDWDVFGDFS"
     gene            6923..7213
                     /locus_tag="ACHJ8C_002600"
     CDS             6923..7213
                     /locus_tag="ACHJ8C_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HFO5552441.1"
                     /translation="MKVKSTLQYFILAFFLSLTASVAYAGGLDTATNTMTELKTWAFG
                     FGGVCALCYLIYNIIMAFMEKKSWSDVGMALIYCSLAGGALYAGNWALELFK"
     gene            7284..7604
                     /locus_tag="ACHJ8C_002601"
     CDS             7284..7604
                     /locus_tag="ACHJ8C_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HFO5552442.1"
                     /translation="MDKVNEHVSESFMTYNGFNRPALVAGIPLMLLLFTAFFAVLTGF
                     PAIFLWGIKGIIIPVICALFLFIVKLACENDSNALRVIRLNLMGLLLKIRHRDLIIGY
                     SSVR"
     gene            7610..9967
                     /locus_tag="ACHJ8C_002602"
     CDS             7610..9967
                     /locus_tag="ACHJ8C_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFO5552443.1"
                     /translation="MHKIKLKNSDNMDVAREYPDYRFHITDSIIFTSDRKMLASLVVA
                     GIPFETENDNVLTNLFNSVKNFLIGLGKEGDLYLWTHLIKKRITIDGEWNFDNAFLSR
                     FSKKYLALFTSSAFYKSTWYLTFGIPYDDVDTGIERMNEMTQQAQKALQPFNASVLSV
                     HNTYISEVADYLSLLLNTEHNMIPLSSTPLSSSICDSEWYFGADVLELRNNESDSKKF
                     ATNYILKDFPIETTPGQWDFLLKQPYEFILTQSFIFESPTKTLKNIDSQLNKLQSAND
                     AAKTQQEELEAGKEAVAAGITLFGSLHCALTVFGDTPDQARSNGIKLSAEFITSGKGF
                     RFSRASLASPFVFFSHMPLNKRRPLDTRRTITNLACLMSFHNYSSGKKSGNPIGDGSA
                     IMPLKTVSDSIYWFNTHYSPPEKNVTGQKIAGHGMILGATGTGKTTFEAAASGFLQRF
                     DPLMFVVDFNRSTELFVRAYGGSYFTLQEGIYTGCNPWQLAEGPESPVWHRLLAFLKR
                     WTQVLARDNQGNPCSDEHGIELNAAVESIMRMPVEERRTALLLDIVSPELQTRLAKWC
                     DNGEYAWAVDSPRNTFNPLHHKKVGFDTTVVLDTKNGIHPACEPLLAVLFFYKEIMQR
                     GGNLMLSIIEEFWMPANFPMTQSMIKSALKAGRMKGEMMWLTSQSPEDAINCAIFAAL
                     VQQTATKILLPNPDAKWEGYKEIGLTEKEFEKLKELTKESRTMLIKQSGSSVFAKMDL
                     FGFDEFIPVLSGSETGLSIFDEIIAEKGDVAPDIWIPELLKRLNG"
     gene            10133..10867
                     /locus_tag="ACHJ8C_002603"
     CDS             10133..10867
                     /locus_tag="ACHJ8C_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virB8 family protein"
                     /protein_id="HFO5552444.1"
                     /translation="MENTNYYEHSKKEATKKKFEEKNEKKDYFKAIRDFERSEIEIIK
                     KKAKTFTILAIGEFVVICILGFAIASLAPLKTAVPFLVRVDNSTGYTDIAPQLSDAKE
                     SYQDVETKYFLSKYLINYEAYDWQTIQEQADTVKTMSSQKVFSAYDTMIRADSSPLNI
                     LKNNYKIKVQINSVILLRKDMAQVRFKKMVLDLSGKPAPGYRATEWISTISFDWDKDI
                     KTEKERLVNPLGLQVLSYQPDPEVIK"
     gene            10933..11634
                     /locus_tag="ACHJ8C_002604"
     CDS             10933..11634
                     /locus_tag="ACHJ8C_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HFO5552445.1"
                     /translation="MYGTPSERDGRIQTVDYNEQDVFNVRVKAGAQTTIKFGQDETIK
                     DVGIGDPEAWSVSVRDNTLFLRPKAEEPDTNVTVQTNKHIYPLYLISTTKQPTYILRF
                     NYPKPPSATVMTEKPFPCTDGGIINGHYQLKGDKSIFPYQVWDNGEFTCMRWTNKQEI
                     PVLYRVDADGNEHLVNGDRNKNTMVYYDVAENLRLRLGDQVADIRTSSIVNRPWNKKG
                     TSNGKTRVEKFSYEK"
     gene            11624..12763
                     /locus_tag="ACHJ8C_002605"
     CDS             11624..12763
                     /locus_tag="ACHJ8C_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFO5552446.1"
                     /translation="MKNKDDEKNNDAGNRGIIEVKGKAAPKKILILIILLIAALFVII
                     ILFKVLSREQVVQQTPLEKSDETLVTNTNNGVSLTTMMKNIEEKEKLDAANRRKAQEE
                     QEQKQADNAPSAPASQKADQTAVNVIANGTPQTASGDPNQPQPLPKSVRQLMGDTMVK
                     IDNQEPGEKNQERDDLQGSQYADGKVSPVLNRRYLLSAGTALSCVLKTKIVTSYPGIT
                     MCQLTRDVWSDNGEVLLARKGALLIGEQNKVMTQGVARVFVNWTTLKDENVNVRIGAL
                     GTDSLGASGLPAWVDNHFGQRFGGALLLSLLGDGLDILKNSTQQTGSNSNITYENTSD
                     ATKEMAKTTLDNTINIPPTAYINQGTVLSVIVPRNIDFSSVYELQ"
     gene            12782..13837
                     /gene="virB11"
                     /locus_tag="ACHJ8C_002606"
     CDS             12782..13837
                     /gene="virB11"
                     /locus_tag="ACHJ8C_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HFO5552447.1"
                     /translation="MNNIRDTSNTSQPRDNSKTARRYLDMTGIQSVLNIEHVTEISVN
                     KPGTIWFEGKNGWESKDAPDATFDNLMTLAKTLTNLSKIKIPLSHDNPIASVVLPGGE
                     RGQIIIPPATENNSVVISIRKPSLTRFTIDDYVRTGRFDNVRIATKHEAILTERQRYL
                     YELSRRPDGQSKAQFLREAVKDRLNFLIVGGTGSGKTTIAKAIADIFPPERRYITVED
                     VPEMSLPLHPNHIRLFYKKNTVEAKEIIEACMRLKPDHIFLAELRGNEAWSYLEALNT
                     GHEGSISTIHANNTYASFSRLASIVKQSDVGMTVDMDLIMRTIKTSIDVILFFNHTRM
                     TELYYEPEEKNRFLSTM"
     gene            13853..15811
                     /locus_tag="ACHJ8C_002607"
     CDS             13853..15811
                     /locus_tag="ACHJ8C_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFO5552448.1"
                     /translation="MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLK
                     WDTFHSVISTFSGNPQYKKLVFMAWFGFSVPLIFFAIFMLIVVIGIMPKKVIYGDARL
                     ATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQFIYFAGQQFLILYAPTRSGKG
                     VGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAETIDQAIKG
                     QIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDK
                     ERFHLEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFF
                     FKFMSAPDRTRGSIETNFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPD
                     ALITHEKIVNLFFSLLVNENCRELPEHNPDLKYQCLILLDEFTSMGKSEVIERAVGFT
                     AGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLYYPPKSKNALAKKISEEIG
                     VRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNIAIREII
                     TSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKA
                     GGKKVMVAGGNVITNPDLDNHDKTDVSE"
     gene            15858..16382
                     /locus_tag="ACHJ8C_002608"
     CDS             15858..16382
                     /locus_tag="ACHJ8C_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO5552449.1"
                     /translation="MRIKHSNMINMNMNMMKEAIDVLINSEKHILIIGETGTGKSTLL
                     AELLINDTDVKYFDFCDIYKRHEHLPLLKHHYLCDENFDYFDFLSAPEKTLVLNSVAI
                     GNNLRESKVVQFIVRFIKTARKRGKRLIVVTTPSDGGVQIQNLFDTVISLTRSHDEIG
                     CTVIIPVPELIKYE"
     gene            16375..18018
                     /locus_tag="ACHJ8C_002609"
     CDS             16375..18018
                     /locus_tag="ACHJ8C_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFO5552450.1"
                     /translation="MNKKLLCLLISSFLLSGCANFKKINANELAAERDAQKATEYAKK
                     IRNNPVIQDSTKQWINTTPIIEKQVKQSAPPCPIVINTRSDIELRQIAQRITQTCHIP
                     VRISADVWTYLNGTGTGSTQQMTGSIPAPDANGMVPLASVGSQPVKVSSGGTSLPELN
                     ISGLPALLQTVSSRLGISWKYDKGRITFFYLETRSFPITYMDSNVAYNSKVVSGTMSS
                     SGSTGSTSSGGMTGDASNTQTTTVEMKSSLYNDLKSEVSSMLTPGTGRMYLSTGSLTV
                     TDTPDVLDSVQEIVNKRNNEMSRQVVLNVEILSIKKSSQEQAGIDWNAVFNDGHLGLS
                     LGGSFGDAAENVITSGVSIVDGKLAGSKAFLKALSSQGSVSVVTQNSAVTKNLTPVPM
                     QIANQQGFIESVTTDSTANVGSSTSLNAATITTGFNMTLLPYIQPDSQNLQLLFSMSL
                     SDKPTFEVFESGGSKAQTPNVDLKTINQTIDLKSGQTVILSGFQQSNRKTSKQGVITP
                     SFFGLGGGINSEDGDTILVVLITPNVLNSGSSAVIKNVN"
     gene            18069..19379
                     /gene="pilO2"
                     /locus_tag="ACHJ8C_002610"
     CDS             18069..19379
                     /gene="pilO2"
                     /locus_tag="ACHJ8C_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFO5552451.1"
                     /translation="MSENNQNNRNFTSVIKNKRAFFSGLDWKTLPSEEKNARTFARKN
                     DAEYFLSCQYQDSENETKTMVAFIRKEDLPTGASSFWSLALMIKPLIEPDGYAICELG
                     DLYGFVSCVNNVLVNDVVGNKSQIMSALTTFLEFNETPEPGWKLYQPESWDISQALPS
                     LTLSALIDVKKPPKEAAFTRVSRKRQFMIYGGSAILAILLWNGITMYQEYREKEAAAE
                     AARLRLAKEMADKQAIQIAPPWQHLPEIKPFIDKCIDKWDALPLSIAGWRFDLAECST
                     SGNDGLLRTSYKELSGVTVEDFSTRIREIFQGTTTATFVLPEGSAGGFSLPVSFDVSP
                     DPITPDTLPQATDIQERLTTFAQKMRLKLTWQEIENTKTDEEGRPIILPWNEYELMIQ
                     TSTPPSILFANFHEPAVRFQYAGIKLEEGRLNYEIKGAFYVKNN"
     gene            19363..19857
                     /gene="pilP"
                     /locus_tag="ACHJ8C_002611"
     CDS             19363..19857
                     /gene="pilP"
                     /locus_tag="ACHJ8C_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFO5552452.1"
                     /translation="MLKIIKPTIISVCFFSSLAYATESESTPKDEIKYSSPTILQVEQ
                     IQAETVIYEAKLARQKAVSELRNLDLNGVASATPAITPSSGYSSLADTDIPAQRVAAK
                     SLRVIEIFGTPQQMSARISLLDGSVTDVKVGQSIPGTSYKVASITGNGVWIQNGTEKR
                     QLIL"
     gene            19882..21420
                     /locus_tag="ACHJ8C_002612"
     CDS             19882..21420
                     /locus_tag="ACHJ8C_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFO5552453.1"
                     /translation="MFFSDADYPGKDISDVVYVSISEKDDKPVQVYIADNSKGQRAVQ
                     AYIGTLNVKYPGQVNITSTTLDVIASRYQDSEKRSDSNSSQHLNSNQEKVISYLAKAN
                     KLGSSDLHITPGRDGSEFTYVEARVHGELEILDVIPRKEGLELLGAAYSGMSDVIKGT
                     QFDPAIPQDARIAENFLKPVNLFGARYSHYPCVGGVYAVFRLIKDDSEDIPTFEELGY
                     MPQQIQTIRRMLQRPEGIIVLSGPTGSGKSTTLRTASEAYLSTFGFNHNDNMRLPRKR
                     LFTIESPPEGRIPGAIQTAVRDSVDGWVDAIKSAMRLDPDAILNGEIRDHASALAAIK
                     ASMTGHLLLTTLHANDALNIIERLEMENIQARLIADPLLFIGLLSQRLVQKLCPYCKR
                     TYAEMADKLSTEERKLIEENCLPEQVRLRNLDGCEHCYKGVTGRTVIAEVVSPDARFF
                     QLYRQSGRLEAKSYWHYELGGITRNQHLLHLINHGLVDPLSAHFISPIDEDKYSMLPE
                     GTWR"
     gene            21411..22520
                     /locus_tag="ACHJ8C_002613"
     CDS             21411..22520
                     /locus_tag="ACHJ8C_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFO5552454.1"
                     /translation="MAIKDIFEKLNSIEIDFSGISHWLAKYTFRYSRISLYEDLSGML
                     NDGINVKRALTHLIDVETDYGKKTGSSATYYICCECLNALNNTGAISSALKKYIKEDE
                     YQLISSGEIRGDLAGGLVQAIKIVRSRSEMTMTLVMSMIYPSVLVFGCLANMYMVHDT
                     ITPVFLSLAPKERWTSSMRLLTDTSGFLIENGTYILCFCIVLFFLIRYSLARYTGPGR
                     QYLDYIPPWSLYKTFNGVSFLFNMASMLSIDIPVAKALERLEKNSTQNRWLLERIKEI
                     RRYVLLGQSLAMAMKSCGYDFPSKLCINKLLLHSENADNALMMSNYADKWLEEAKGKV
                     KSTGVWITVVSALIVFAFIVMMITALYDVSNVLQH"
     gene            22565..23122
                     /locus_tag="ACHJ8C_002614"
     CDS             22565..23122
                     /locus_tag="ACHJ8C_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFO5552455.1"
                     /translation="MSSINILNMRSVFSSLSARRKKEQDKGATLMEVLLVVGVIVVLA
                     ASAYKLYSMVQSNIQSSNEQNNVLTVIANMKSLKFQGRYTDSNYIKTLYAQGLLPSDM
                     IADTTGASAKNPWGGSVTITTSSDKYSFNVVEASVPKKNCMAMVNALRSSSAISKINN
                     TSTSTVDAATVCSSDSNTLTFSTDS"
     gene            23189..23671
                     /locus_tag="ACHJ8C_002615"
     CDS             23189..23671
                     /locus_tag="ACHJ8C_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO5552456.1"
                     /translation="MRSVLYILLSCISSLIFSLQPAYSATTTRFDACFAQAGQRYLID
                     PLLLKSIATVESSLIPDRINHNKEKGKILSTDYGLMQINSSHIPNLKKLGVIKDKSEL
                     IDNPCLNIQIGAWILATHFQKCGINWSCLGSYNAGFKESNEQKRIKYARYVYNKYMVR
                     "
     gene            23675..24310
                     /locus_tag="ACHJ8C_002616"
     CDS             23675..24310
                     /locus_tag="ACHJ8C_002616"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFO5552457.1"
                     /translation="MLNSIFNTAILCCANIVCQCYAYNEVKFRLNKLNVSYKTGTEKY
                     YCLILTTLAVLYLSLNQLSLIQSIIVIVFYAFLTLMACIDLLSFLLPRLYTVTFIFSG
                     LLYQTWNNNILSGLFCAMLMFFIMLFVRLYFAYKNGTESFGMGDVLLIAGTGVWFPTP
                     EIACSIVFIAVIGGIIFFTLGGLNKQKKYIPFGPFLCGGMFVYSLVPGILF"
     gene            24323..>25336
                     /gene="pilV"
                     /locus_tag="ACHJ8C_002617"
     CDS             24323..>25336
                     /gene="pilV"
                     /locus_tag="ACHJ8C_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFO5552458.1"
                     /translation="MKKTDKGVSLLEVLLVIGIMVMVIPKVYENIENHLNNVRWQNAA
                     EHANTYNTAVRNYVADNASTLLAGSLPKTITPATLIQKGYLKSGFSESNFGQSYITGI
                     AKNSKTSRLEALTCSNGGQSLSEAGMRSVASMIEGLGGYINSSKQAIGAGGGWSDTPS
                     NYGLNCATGHIAMALVGADLQESDRLYRYSITNRPDLNRMHTAIDMNSNNLNNVGTLN
                     GNAAALSGDISARNGTFSGAISGNTATTNGDITSNNGWLVTKNSKGWMNSTYGGGWYM
                     SDSSWLRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLEKTATAGTSCSPNGLVGRD
                     STGA"
BASE COUNT         7865 a         5482 c         5246 g         6743 t
ORIGIN      
        1 catctcataa tagccacgct ctcgattaca tcattaaaaa agcgtttaca ccacgcaaaa
       61 ccaaaacatc gacagcgacc cacaacgttt atagttacaa taaagaagaa tccacattaa
      121 atttccaaaa aattggagaa gcaatttttt tcggcacaga cctggtaatt aagctgaagc
      181 caaatattag tgcctctggt gaactcttca tctgccagaa ggactaagct atgaacattg
      241 tttcagaatt tcttgagaaa gtaacaacca ttgttgaatc cacagcggcg acgaacgccg
      301 ctaaggtcgc acaggcaata tctcctacat tttttgcggc tatagcctta tacgttattt
      361 atctaatata tgaaatcacg tatgcccaac gtgacatcct tatgaacgag gtgacgaaga
      421 atatcggtgc attcgccctg gttggtgcat tcacttattc agcgccttat tacagccagt
      481 ttgttattcc tttcgtaatg cacgctggct ccgacctttc tgctgctgtt actggaggtt
      541 caggaactgc aaccagtgtt gataatttgt ggaatatgct ttcagtaacg ttaaatgatt
      601 ttaagaataa tgagttagat actctggagt ggtatagctt cacagatcaa ctatacatat
      661 atctaatttg gggggttgga tatgttggcg ggctgctgct tatttattac accactgtat
      721 ttctaactct atcaacattc atggttggca ttctcttatc cgccggaata ttatttatct
      781 gtttttctct atttgcttcg acaagaaata tgtttagcgc atgggtaggt agctgtttaa
      841 attatatatt gctaaatctt ttttatagta tatcgttcag cttcgtcatc agctttgtag
      901 agcaaacagt cccatcaagc ggaaatataa ctctcacaac agtagtttat ttctttatgg
      961 tgactgttat atctatattc cttgttgagc aagtaggtac tctctgctca actcttactg
     1021 gcggggtagg tataaatggc cttacatcag ccgctaatgg ctttggaggt aaggttgcat
     1081 ccggttttat gcgagcatca ggactccgat ctttcactaa tggttttgcc agcaaaatgc
     1141 caaatccatt ctttaatgca ggaagtcgtt ctgcatcagc attagttcag ggattaaacc
     1201 gtggaagtaa acttaaagcg gggtgattaa catgaaagat aaagtattat catttctctt
     1261 tgctggaata attaccataa catcaccatc aatctttgca tctggtattc cagtagtaga
     1321 cgttgcagca atagctaaaa cagttgaaga agggctaaat cgtgctgctg aagcggcaag
     1381 acagcttgag caactcaagc aacaatatga acagaccata agatacgctg aagaacagaa
     1441 gaaaagactc gaaggcttca ctgatttcag caatggcttt gatagcgcat ccagctatat
     1501 gaaggattct ctatcaacaa taacaaatag tgcaaaatca gatcttagct ctttgcgatc
     1561 gcaatatgac ttatcaagca acgttgcaga gacgcaacaa aaatatgatg cgatcctggc
     1621 taagatcaaa ttctatgaaa atttcaacaa tgaaatgcag gaaagggcta agagattaac
     1681 cacgttacag aaggagttcg cgtcagcaga tactccgcaa aaaaaagctg acttatctaa
     1741 ccaattgaat acagaaaaat tgacaatgga gctacaattg aagcaatatg atattgctga
     1801 acggcaacta gagaatgagc ggaaagctca atatgaaaca tatgttcgcc aaaagagaag
     1861 agatttaagc tgatatgaat aagtcacgaa atgcaattat cgcctcatca attatcttcg
     1921 ccagtttaat gttagtggga tgtaaagaaa aaatatattc tgtagaatat tactcaaaca
     1981 acatcagtga agccactaaa actttagaag attgcaagaa aggtacaata accgatcaaa
     2041 actgcgataa tgctcgtgct gcacttcagc aaaaacaaga tagtgaatat aaaaagaaag
     2101 ttagtgaaat gagaagacgc cttgattaag gaatacataa aatgccaacg aagcactcta
     2161 tgatctaaaa cgaaaagaaa tcatgagtca aatgcttgga cgctccaata aataaactta
     2221 tgccatgata aaaaacccgc ctcgtgcggg ttttatcctg cttaaagatc tatgaaacct
     2281 ccggcttcgg gtattaaact ataacttgct ttggcaaata ttgtttcatt gacttcatgg
     2341 aatcttattt tctccgcctg ttcatatgtt acagggaaga acacgccccc tggctcatta
     2401 aacatcataa aatcaccttg cattgcgtta caatgacaac aatgattcgc gtaatacttc
     2461 atttttgctg ttttgctatg ggatggataa tataattttg aatactcatt aatccttgag
     2521 agagcagagg ttgaaattat ttcaattttc gttaaatcag tccaccatac tctactggtt
     2581 aaccaccgat aaatcatatt tttctttgaa tcaaaatact ccataccctt aggggaggat
     2641 gtttcatacc attcatctaa tatacaatct tcccattcgg caggatcaac tccccaaata
     2701 acgtctctat acttatacca acgaggtaaa aaaataccaa acacaggagt tgttttctta
     2761 cagcgccaac aagactggta actttccgca atataaaaac gttgcgaaaa aacattgtaa
     2821 tcatgttcat ttaattcttt tatccaccga ataaaataaa ttggattttt tccctgtgga
     2881 acaaaccatt tccgttgctc ttggtcccat tgcgcccctt tttgtttagc caaatccttt
     2941 tcttcaaacg gaacatttaa ataaaaccgc gccatagcca acttaactcc agtattctgt
     3001 tctgatgatg cacctggata gatatcaaaa ctaaacaaag agtgcaattc cgcctctaat
     3061 ggctcattca tacccgcctc gtgcgggttt tcttttgcct gaaatatgga ggaactccat
     3121 gaaatccatc attttatcga tggttgccgc caccgcatta ctgtcaggct gcgcggatag
     3181 caaaacacaa ccagtacctg agtcaccgtt aacctcaaat gaccaccaga taacgttgaa
     3241 aattgatacc gtgcgaacag acctgccgga cagttctcct gtctgtttga atggtatgaa
     3301 tatatacgaa agtctgagcg agcggcatcc aggcttgttt gccaaaaatc agaacgtgat
     3361 tttccgtagt gcattctttg atgacggtca ggcgatatcc tccgaaacaa tccgcaaaat
     3421 aatgcagagt accccgtgcg ttgaatcaca aacaacatct gccggaagct gggaacctgc
     3481 cacggaactg atcccccgtc aggcaatatc cggtgacact acactggctg aaaaagatcg
     3541 gactgctggc ttcacaatgc ggatagcacc ggagattgag cgtggaaatt tccacctgct
     3601 actcacaacc actgacccga tacggaaaca gactgtcgag cagaatattt tactcaaacc
     3661 ggaccagccc cttatcgtga gtggttttac tggagttaat gctgatagcc aaagtgagaa
     3721 ccggattgtc atcattacgc cacacgtgcg ttgattcccc tgatatttaa attacccgcc
     3781 tcgtgcgggt tttcttttgt ctgaaatatg gaggaacacc atgaaatcca tcattttatc
     3841 gatggttgcc gcaagtgcta tcgcactctc tgcccccgta tatgcgtctg atccgtgtgc
     3901 ttccgttctt tgtctgtacg gtaaagctat cggtagcggc ggcgggagcg aatgcaaaag
     3961 cgccgaaaag gacttcttta atatcataaa aaagaaaaag ggatctattc gctggagcaa
     4021 aacctttgat gcccgaaaag cgtttcttaa tcaatgcacg accgcagacc ctgcggcgat
     4081 ctcaaaaatc atgagtaagt tcggacgttc ctggggttaa ccatgaaaaa gctcattatt
     4141 attcccacca cattattgct gaatgcgtgt tcccatgccc ctgaacccgc acaggcaaaa
     4201 ggaggttggt atgaactgaa taccacaatt gagcaggtaa gagcagacac atactataac
     4261 ggggtgatcc ttcagcagcc cgtgtcacag ccaaacagca ctgttcaata cagcaatgat
     4321 aattatctca aaaaggaagc cataaacgta actcccgcgc ctcgtattgc agaaaccgta
     4381 aaaccacgtt ctgagcaatc agcgacagcg ccaaaaaacc agggcagcaa taatagtttt
     4441 ctgaagccga caacaaacag cataacttca tctggtgaga taaaaacagc agccaaagga
     4501 gcaactaaac agcattctga acaaccagca ttctctcccg caaagccaat cagtagcaac
     4561 aaccatcagg cacaaacggc acaaaagact gatcagcaga caaaaccgcg tcctgaacaa
     4621 ccaccgctaa tgtcaaaaag cggttttctg aaaaaaaacg ctaacaacgc tgttccggcc
     4681 accacgctta caggcccatc cacacctgaa aagcaaattt actttgttcg ccatgatggt
     4741 aaaggctcgc tgcaaaaagc attaaaagaa attgtcccat cggactggac ggctgaaata
     4801 tccctggagg taacgaaaac tttccgtcgc acgatatcct ggcagggcaa cgatcaatgg
     4861 ccttatgtcc tggataaaat gcttcgcaac tacggtctga cggtaattaa gaacaccgaa
     4921 aaaagacatc ttctgattga ttttcaacaa cagaaaggga aaacatcaca aaaagcacct
     4981 gtatctcctg acaaaacccc tgcatctcct gtcaggacac cagtttcctc tgacaagtta
     5041 cctcaaaaac cgctaacgcc tgttaccgtt ccgcaaaaac agacagaaaa actcacctgg
     5101 aatcttaaaa aaggttcgac gctgcgggat ggcctcacag aatgggcatc aagttcgccg
     5161 tgtggtaatt caacctggaa tcttgtatgg gatactcctg tgaactacag aatagatgct
     5221 cctcttattt tccggggaaa ctttgaatct gcattaaaag atacgctgat gctttatctt
     5281 tcagcagata aaccgttata cgcattcagg aatattgcac aatgcgtaat cacaattaaa
     5341 gacacacaac aggagtaatc gctatgggtt ggttcttcag cttgctcctg atattcctgt
     5401 caataggcag ttactatctg acagataaca ataaccaaac catacagcga atgaagttgc
     5461 ttcctgcaaa acaggaagca attgaatttg tccatttcgc aaatgttatc aatgactatc
     5521 tttataaata tccagacaaa cgtaattcag gaggaacgtt aacatctgag caaatcggta
     5581 taacacccgt ttatgatatt catcatatta tttacggtaa gcgagtttat atctggtctg
     5641 ccgatacaga aggtctgatg tctgctttgc agcagcaaac aaaacattca gcaatgttag
     5701 gaagagtaaa aaataagaag attgtcgata atcaggggaa tgatatgggc gttcccgttc
     5761 cttcctccat tccagaagag gctattgtac tcattaacta aatatcccct gacaagggga
     5821 ttctgaactg gagtctgcat atccgacaca tcattatgtg ggccggatat atcgctacgg
     5881 cgcggacttc agtccagaaa atctttaccg gagataccca tgcaggataa tgatgatgtc
     5941 agcgagcttg atattgccac tcgtgaagtc cttatcttac gatgcaatct tattcagctt
     6001 gcttctgaat atataaaaat aaatcctctt aaaaacaccc gaatctacac agatttaatt
     6061 catcgttcag cagatgttat caaaaatatc gaacaaggta acatccagaa taaacataat
     6121 tatctgtcac aggatgtaaa acatacgctt gttttatacg acctgtacaa atcagacttt
     6181 gaacaatgtc cgggggcaaa atcccttata cctgtcaggg aaaaaattgt ctcgctgaaa
     6241 gaacttcttt ccggcgctaa tgagctggac agtatcgttc gtcgcccccg caggaaaaaa
     6301 cgttcttcac gaatttaata tcaggagata aatcccttat gcagttatca gcggctgttc
     6361 tggcagcact gataagtcag tgtgcccctg atgtatcacc cgatacgatg aatgccctga
     6421 ttatgactga atcaggggct aatccatatg taatagcgaa tgtttctgac ggaacaagta
     6481 agtattttaa agatgaaaaa ggagctattg aatatgcaga aaaattaaca gcagaaaata
     6541 aaaggtttag tgcaggtctg acacagatat acagcaaaaa tttcccatca ctcaatctga
     6601 ctaataaaac agtttttgac ccgtgtacaa atataaaagc aggagcggca gtcctgacgg
     6661 acaattatct gagacaaaag gagggtagta gtaatcaaaa aattctccgc gctttatctt
     6721 tgtattattc gggtaacgaa tcaacaggat ttattaaaga aaagaaattt aacaacactt
     6781 cttatctgga acgggttatt cgtaacgcga ataactatat cgtgccatca atccgtgaaa
     6841 aaactgatga atcagaaata aaacccccga atgatgaaga gaaaacatca ccggactggg
     6901 atgtttttgg agattttagt taatgaaagt caaatcaact ttgcagtatt ttatcctggc
     6961 gtttttcctg tcactaaccg cctccgttgc ctatgcaggt ggactcgaca ccgcaacaaa
     7021 taccatgaca gaacttaaga catgggcatt tggctttggt ggtgtttgtg ctttgtgtta
     7081 cctgatctat aacatcatca tggcattcat ggagaagaaa agctggtctg atgttggaat
     7141 ggcgcttatc tattgttccc tcgctggcgg tgcactttac gccgggaact gggcgctgga
     7201 actcttcaaa taagtaatcc atatatacaa ctgtgccgca tatttacctt atgtgtgaat
     7261 tgcggctttt ttcatggtga gttatggaca aagtaaatga acatgtcagt gagtcattca
     7321 tgacttataa cgggtttaac cgtcctgcgt tagttgctgg tataccctta atgttgttat
     7381 tgttcacggc ttttttcgct gttctgacag gcttccccgc tattttcctt tggggtataa
     7441 aagggataat tattcctgtc atatgtgcgc tgtttctttt tatagtaaaa ctggcctgtg
     7501 aaaatgactc aaacgcacta agagtcatcc gtttaaacct tatggggctt ttgttaaaaa
     7561 tccgccacag agatttaatt attggttatt catctgtcag gtaacatata tgcacaaaat
     7621 caaattaaaa aacagtgaca atatggatgt tgctcgtgaa tatcccgatt atcgcttcca
     7681 tataacagat agtattatct ttacttctga caggaaaatg ctggcttcac ttgttgtagc
     7741 aggcattcca tttgagacag agaacgataa tgttcttaca aatttattta acagcgttaa
     7801 aaacttcctg attgggttag gaaaggaagg cgacctgtat ttatggactc atcttatcaa
     7861 aaaaagaata accattgatg gagaatggaa ttttgacaat gcttttttaa gcaggttttc
     7921 aaaaaaatac cttgctctgt tcacatccag cgcattctac aaatcaacat ggtatttaac
     7981 atttgggatt ccctacgatg atgttgatac cggaatcgag cgcatgaatg aaatgacaca
     8041 acaggcgcaa aaagcacttc agccatttaa cgcttctgtt ttatcagttc acaatactta
     8101 tattagtgag gttgcagact atttatcact attgctaaat acagaacata acatgattcc
     8161 tttatcatcc actcccttat catccagtat ttgtgattct gaatggtatt ttggtgctga
     8221 tgttcttgaa ctgcgtaaca acgaatcgga tagtaaaaag tttgcaacga attatatttt
     8281 gaaggatttc cctatagaaa cgacccccgg acaatgggat tttcttctca agcaacccta
     8341 tgaatttatt cttactcaat cgtttatttt tgaatcaccc acaaaaaccc tgaagaacat
     8401 tgattcgcaa ttaaacaaac tgcaatcagc taatgatgca gccaaaacac aacaggaaga
     8461 actcgaagct ggtaaagaag cggtcgcagc cggaattacg ttgtttggtt cactacactg
     8521 cgcattaacg gtattcggtg atacaccaga tcaggccaga agcaatggta taaagctctc
     8581 tgctgagttt attacttcgg gtaagggatt ccgcttcagt cgcgcctcac tcgcatcacc
     8641 ttttgttttt ttcagtcata tgccgctgaa taagaggcgt ccactggata cccgaagaac
     8701 aataaccaac ctggcctgtc tgatgtcttt tcacaattat tcctccggga aaaaatcagg
     8761 caatcctatt ggtgatgggt ccgccataat gccgttgaaa acggtgtctg acagcattta
     8821 ctggttcaat acgcattaca gcccaccgga aaaaaatgtt accggacaaa aaattgccgg
     8881 gcatggaatg attcttggcg caacaggcac agggaaaaca acatttgaag cagcagccag
     8941 cggttttctt caacgctttg accctttaat gtttgttgtg gatttcaacc gttctactga
     9001 gctttttgtc cgtgcatacg gaggaagcta tttcacgttg caggaaggca tttataccgg
     9061 ctgcaatccg tggcaactgg cagaaggccc ggaatctcct gtctggcaca gacttctcgc
     9121 atttctgaaa cgctggacac aggttctggc acgagacaac cagggcaacc cctgttctga
     9181 tgaacatggc attgaattaa acgctgccgt ggaaagcatt atgcgaatgc ctgtcgaaga
     9241 acgccggaca gcccttcttc tcgatatagt cagtcctgaa cttcagacgc gtctcgccaa
     9301 atggtgcgac aacggcgaat atgcctgggc ggtggattcc ccccggaata ccttcaatcc
     9361 actgcatcat aaaaaagtgg gatttgatac aaccgttgtc ctggacacaa aaaacggtat
     9421 tcatcctgcc tgtgaacctt tactcgcggt cctgtttttc tacaaagaaa tcatgcaacg
     9481 cggcggcaat ctgatgctct ccattattga agagttctgg atgccagcaa actttcccat
     9541 gacacaaagc atgattaaaa gcgcactgaa agcggggcgc atgaaaggtg aaatgatgtg
     9601 gttaacatca cagtccccgg aagacgccat aaactgcgca atttttgcgg cgctggtaca
     9661 acaaaccgca acgaaaattc tcctgcccaa tcccgatgca aaatgggaag gatataaaga
     9721 aattgggctt actgaaaaag agtttgaaaa actcaaagag ctgacgaagg agtcaaggac
     9781 catgctcatc aagcaatctg gctcttctgt tttcgccaaa atggatttgt ttggattcga
     9841 tgagtttatc cccgtgcttt ctggctctga aactggcctg tctatttttg atgagattat
     9901 tgctgaaaaa ggagatgtag ctcctgatat ctggatacca gaactcctga aacgcctgaa
     9961 cggttaattt ctctgtttta tacagaacgg aaaacaatat gaaaatcccc ctcataattg
    10021 cctccgcgct attaattagt gcatgttctc acacccccaa tcctgtgcag aaaaaagggg
    10081 gatggtttga gttaaacaca gcaattgaac agatcaaagc aggtgattat taatggaaaa
    10141 cacaaactat tatgaacatt caaaaaaaga agccaccaaa aagaaatttg aagaaaaaaa
    10201 cgaaaaaaag gattatttca aggccatacg tgactttgag cgcagtgaaa ttgaaatcat
    10261 aaagaaaaag gcaaaaacat ttaccattct cgccattggt gaatttgttg taatttgcat
    10321 tcttggtttc gcaatagcct cccttgctcc attaaaaacc gctgtaccgt tccttgtgcg
    10381 agtggataac agcactggat atactgatat tgcccctcaa ttatctgatg ctaaggaaag
    10441 ttatcaggat gttgagacta aatatttcct gtcaaaatat ctcattaact atgaagcata
    10501 cgactggcag accatccagg aacaggccga tacagtaaaa acaatgtctt cccaaaaggt
    10561 gttttcagct tatgacacca tgattcgtgc tgattcctca ccactgaaca ttctgaaaaa
    10621 caactacaaa attaaggtcc agattaattc agtaatcctt ctaaggaagg atatggcgca
    10681 ggtgcgtttc aaaaaaatgg tactggattt atctggtaaa cccgcccccg gatacagagc
    10741 aacagagtgg atttcgacga tttcttttga ctgggataaa gatatcaaaa cagaaaaaga
    10801 gcgccttgtg aatccgctgg gactgcaagt tctgagctac caacctgatc cagaggttat
    10861 aaaatgatgc ttaaacgcac tactgcgata gttcttttgt cgatgctaca agtacctggt
    10921 gctttagcag ctatgtacgg cacaccatcg gaacgagacg ggcgtattca gaccgtagat
    10981 tataacgagc aggatgtatt taatgtgcgc gtaaaagcag gcgcacaaac aacaattaag
    11041 ttcggacaag acgaaacaat aaaagatgtc ggaattggtg atccagaagc ctggtcagta
    11101 tcggtcaggg acaacactct gtttctgcgt ccaaaagcag aagaaccgga tacaaacgtt
    11161 accgttcaga ccaataagca tatatatcct ctttacctga tatcaaccac gaaacaaccc
    11221 acgtatattc tgcgttttaa ttatccgaaa ccaccgtcag ctaccgtaat gactgaaaag
    11281 ccatttccat gcactgatgg tggaataatt aatggtcatt atcaactcaa gggggacaaa
    11341 tcaattttcc cttatcaggt atgggataat ggcgaattta catgcatgag atggactaat
    11401 aaacaggaaa ttccggtcct ttatcgtgtt gatgccgatg gaaatgaaca tctggttaac
    11461 ggagacagaa ataaaaacac aatggtttac tatgacgtgg ctgaaaatct ccgtttgcgc
    11521 ctgggtgatc aggttgcaga tatcagaaca tcatcaatcg ttaatcgtcc gtggaataaa
    11581 aaaggtacgt caaacggaaa aacacgagta gagaagttca gctatgaaaa ataaagatga
    11641 tgaaaaaaat aatgacgcgg gaaatcgcgg aattatcgaa gtaaaaggta aagcagcacc
    11701 aaaaaaaata ttaatcctca tcatattact gattgccgcg ttatttgtga tcattatctt
    11761 gtttaaagtt ctgtcacgag aacaggtcgt tcagcaaaca cctcttgaga agtctgacga
    11821 gacactggtc acaaatacca acaatggcgt ttcactgaca acaatgatga aaaacatcga
    11881 agaaaaggaa aaactcgatg ccgctaaccg caggaaagca caggaggagc aagaacagaa
    11941 acaggcagac aatgccccgt cagcgccagc atcacaaaag gccgatcaaa cagccgtcaa
    12001 tgtgatagcc aacggcactc cccaaaccgc cagtggagat ccaaaccaac cacaaccatt
    12061 gccaaaatcc gtccgacaac tgatgggcga cacaatggtg aagatcgaca atcaggaacc
    12121 aggcgaaaaa aaccaggaaa gagatgattt acagggcagt cagtacgcag acggaaaggt
    12181 atcccctgtg ctgaaccgcc gatatctgtt atctgcggga acagcattat cctgtgtgct
    12241 caaaacgaaa atcgtaacct cttatcccgg tatcacaatg tgtcagttaa cacgagacgt
    12301 ctggtcggat aacggcgaag tgcttcttgc ccgtaaagga gcattactga tcggggagca
    12361 gaataaagtc atgacacagg gagtagcccg tgtgttcgtt aactggacaa cgctcaaaga
    12421 tgaaaatgtc aatgtgcgca ttggcgcact tggcactgac agtctgggcg cttccggcct
    12481 tcctgcatgg gtcgataatc attttggtca gcgttttggt ggtgcattgc tgctgtcatt
    12541 acttggtgat ggtttagata ttctgaaaaa cagcacgcaa caaacaggct caaacagcaa
    12601 tatcacctac gaaaacacat ctgacgcaac aaaagaaatg gcaaaaacaa cgctggacaa
    12661 tacgataaat attcccccca cggcatatat taatcagggg acggtattgt ctgttattgt
    12721 tccgcgaaat atcgactttt catccgtata tgaacttcag taattcagta aagcaattga
    12781 tatgaataac atcagagata ccagtaatac atcacaacca cgagataaca gtaaaaccgc
    12841 aagacgatat ctggatatga cgggtatcca gtctgttctt aatattgaac atgtcacaga
    12901 aatatctgtg aataaaccag gaacaatatg gtttgaaggg aaaaatggct gggaatctaa
    12961 agatgcgcca gacgcgactt ttgataattt aatgacactt gccaaaactc tgacgaactt
    13021 atcaaagata aaaataccac ttagccatga taatcccatt gcatcggtag ttcttcccgg
    13081 tggggaacgt ggacaaatca tcattccacc tgcaacagaa aataacagcg ttgtgatatc
    13141 tatccgtaag ccatcattaa cgcgctttac tatagatgat tatgtccgaa cagggcgttt
    13201 tgataatgtc aggattgcga cgaaacacga agccatcctg actgaacggc aacgctacct
    13261 gtatgaatta tcccgacgtc cagatggtca atctaaagct caatttttaa gagaggcagt
    13321 taaagaccgt cttaattttt taatcgtagg tgggactggc tccggtaaga caactatagc
    13381 aaaggcaatt gcagatatct ttccacctga acggcgttat ataaccgttg aagatgtacc
    13441 ggaaatgtct ctgccgctgc atccaaacca catccggctt ttttacaaaa aaaataccgt
    13501 tgaagcgaag gaaatcattg aagcgtgtat gcgattaaag ccagatcaca tctttcttgc
    13561 tgaacttagg ggtaacgaag catggagtta tctggaggcg cttaatacag ggcatgaagg
    13621 ctcaatcagt accattcatg ctaataatac ttatgcctca ttttcacgac tggcaagtat
    13681 tgtaaaacaa tctgatgttg gtatgactgt tgatatggac ctcatcatgc gcacgataaa
    13741 aacatcaatt gatgtcattc tttttttcaa ccataccaga atgactgaac tgtattacga
    13801 acccgaagaa aaaaatcgct ttttgagcac aatgtaaatt ctggagataa ctatggatgc
    13861 aaaaaagacg ggggggttaa ttctcttcct gttactgctt cttgtgggtg tgcttatagc
    13921 ctcaaactat ctcggtggat acacagcatt aagatattca tccgttgata tgtctttgct
    13981 caagtgggat acattccatt ctgtaatctc aacattttct ggcaacccac aatataaaaa
    14041 actcgttttt atggcatggt ttggattcag cgttccatta atattctttg ccatattcat
    14101 gttgattgtc gtgattggga ttatgcctaa aaaagtcatc tacggtgacg cccggctcgc
    14161 aactgatatg gacctgagta aatcaggttt tttcccggat aaaaaatcac cttacaaaca
    14221 tccaccaata ctcatcggta aaatgttcaa ggggagatac aaaaaacaat ttatttattt
    14281 cgccggacaa caatttttaa ttctttatgc cccgacacgt tctggtaaag gggtggggat
    14341 tgttatcccc aactgcgtaa actatccagg aagtatggtc atactggata tcaaacttga
    14401 aaactggttt ttatcagccg gattcaggca aaaagaatta gggcaagaat gctttctctt
    14461 tgcgccagcc ggatacgcgg aaactatcga ccaggccatt aaaggacaga tacgctctca
    14521 ccgctggaat cctcttgatt gcgtcagtcg cagtgattta cttcgtgaaa cggacctggc
    14581 aaaaattgct gcaatcctta tcccggcaag tgatgatcct atatggtctg attctgcacg
    14641 aaaccttttt gtcgggttag gactttacct gctggacaag gagcgttttc acctggaaca
    14701 gaaagccaaa ggtcataatg taccggacgt tcttgtttcc atcagcgcca tactgaaaac
    14761 gtcggttccc gatggcggca aggatctcgc cgcatggatg ggacaggaaa ttgaaaaccg
    14821 tagctggatt agcgataaaa caaaatcatt cttctttaag ttcatgtcag caccagatcg
    14881 cacaagggga agtatagaaa caaacttttc atcaccgctg agcatcttca gcaaccctat
    14941 tactgctgag gcgacgaatt tcagtgattt tgatatccgg gatattcgta aaaagcccat
    15001 gtcaatttat cttggtctga caccggatgc tttaatcact catgagaaaa ttgtaaacct
    15061 tttcttctca ttactggtta atgaaaactg tcgtgaacta ccggaacaca acccggacct
    15121 gaaataccag tgtcttattc tcctggatga atttacatca atgggtaaat cggaggtcat
    15181 tgaacgcgct gttggtttta cagcaggtta taaccttcgc tttatgttca ttcttcagaa
    15241 tgaaggacaa gggcaaaaaa gcgatatgta cggtcaggaa ggatggacca cattcactga
    15301 aaacagtgct gttgtgcttt actatccgcc gaaatcaaaa aatgctctgg ctaaaaagat
    15361 ttcagaagag attggggtgc gtgatatgaa aatatccaaa cgctcaatat ccagtggtgg
    15421 tggtaaggga ggaagttcac gtacacggaa tgatgatgta atagagcgcc cggttcttct
    15481 tcctgaagaa attgtgtctc tccgggataa gaagaataaa gccagaaata ttgcaatccg
    15541 tgaaattata accagtgaat ttagccgacc atttattgcc aacaaaataa tctggtttga
    15601 agaaccagag tttaaacgcc gcgtggatat cgcccgtaac aatcatgttg acattccaaa
    15661 cctgtttaca caagaagtca tggatgaaat agcaaaaatt gcagaaattt atctccctaa
    15721 agctggagga aaaaaagtca tggttgccgg aggcaatgtg attactaatc cagaccttga
    15781 taatcacgat aaaacagacg tatctgaata atcctcgcaa taaagatact attgattagc
    15841 aaagacagga ttctattatg agaataaaac actcgaatat gataaatatg aatatgaata
    15901 tgatgaaaga agccattgat gttttgataa actctgaaaa acatattctg attataggcg
    15961 aaactggaac tggaaaatca acacttttag cagaattgct aatcaatgac actgacgtta
    16021 aatattttga tttctgtgat atttataaac gccatgaaca ccttcccctt ctaaaacacc
    16081 attatctttg cgatgaaaat tttgattatt ttgacttttt atctgcacct gaaaaaacgt
    16141 tggttcttaa tagtgtagcc atcggtaaca acttacggga atcaaaagtt gttcagttta
    16201 ttgttcgatt tattaaaaca gccaggaaac gtggcaaacg tcttattgtt gtcacgactc
    16261 cttcagatgg cggcgtgcag atacaaaatc ttttcgacac agttatatcg ttaacccgct
    16321 ctcacgatga aataggatgc acagttatca ttccagtacc ggagttaatt aaatatgaat
    16381 aaaaaattgc tttgtttatt aatatcctct tttttattat caggctgtgc aaacttcaaa
    16441 aaaatcaatg ctaatgaact tgcagccgaa agagacgcac aaaaagcgac tgaatacgcg
    16501 aaaaaaattc gtaataaccc ggttatccag gactcaacaa aacaatggat taatacaacg
    16561 ccaattattg aaaagcaggt aaagcaatca gcgccgccct gcccgattgt gattaatact
    16621 cgttcagata ttgagctacg ccagatcgca caacgcatca cacaaacctg tcatatcccg
    16681 gtacgaatca gcgccgatgt ctggacctat ctgaacggta caggcactgg ctcaacacaa
    16741 caaatgacag gatcaattcc agcaccggat gcaaatggaa tggttcctct ggcgtcagta
    16801 ggcagtcagc cagtaaaagt ctcttctggc ggtacatcac tcccggaact gaatatctcc
    16861 gggcttcctg ccctactgca aaccgtatct tcacggctgg gaatatcctg gaaatacgat
    16921 aaaggtcgta tcacattctt ctatctggaa acgcgctcat tcccgatcac atatatggac
    16981 agcaatgtgg catacaactc aaaagttgtg agcggcacaa tgtcttccag tggcagtacc
    17041 ggctctacca gttctggcgg tatgactggc gacgcatcaa atacgcaaac tacaaccgtg
    17101 gaaatgaaat catcgctgta caacgacctg aaaagcgaag tttcctcaat gctgacacca
    17161 ggtacagggc gcatgtatct gtccacaggt tcactgacag taacagatac cccggatgta
    17221 ctggacagcg ttcaggagat tgtgaacaag cgcaataatg aaatgagtcg tcaggttgtg
    17281 ctgaacgttg aaatcctgag tatcaagaaa tcaagccagg aacaggcggg aatcgactgg
    17341 aacgcagtat ttaatgatgg tcatcttggt ctgagtcttg gtggctcatt tggtgatgcc
    17401 gccgagaatg ttattaccag tggtgtatcc atcgtggacg gtaagctggc tggctcaaaa
    17461 gcatttctga aagccctgtc gtcacaaggc agcgtcagtg tcgtaacgca aaactccgct
    17521 gtgactaaaa acctgacgcc tgtccctatg caaattgcca atcagcaggg cttcattgaa
    17581 agtgtcacaa ccgattctac cgccaatgtg ggatcatcca cgtcactgaa tgcggcaaca
    17641 ataacaaccg gattcaatat gactctgctt ccatacattc agccggattc acaaaacctg
    17701 caattgctgt tctcaatgag tctgtctgac aaacctactt ttgaggtgtt cgaaagtggc
    17761 ggcagtaaag cacaaacacc gaatgtggat ttgaaaacaa ttaatcagac tattgacctg
    17821 aaatcaggcc agacagttat tctgagtgga ttccagcaat caaaccggaa aaccagtaag
    17881 caaggtgtaa taaccccctc cttctttgga cttgggggtg gcattaacag cgaagacggc
    17941 gacacgattc tggtcgtttt gattacacca aatgtgctta attccggctc cagtgcagta
    18001 ataaaaaatg tgaactaatt aactgaaaac tccctgaaaa aacacaggga gtttttttgg
    18061 aggtcacttt gtctgagaat aatcagaata accggaattt cacttcggtt attaaaaaca
    18121 agcgtgcttt tttctccggt ctggactgga aaacgcttcc ttcagaagaa aagaatgcgc
    18181 gtacattcgc aagaaaaaac gacgctgaat attttctctc ctgtcagtat caggattcag
    18241 aaaatgaaac caaaacgatg gtggcattta tcagaaaaga ggatcttccc acaggggcgt
    18301 cctcattctg gtcactggca ctgatgataa aacccctcat cgaacctgac ggatacgcta
    18361 tctgcgaact cggtgatctt tatggctttg tttcctgcgt caataacgtt ctcgtcaacg
    18421 atgttgtagg taacaaaagt cagatcatga gtgctctgac aacatttctt gagtttaatg
    18481 aaacaccaga acccggctgg aaactatacc agcctgaatc atgggatatc agccaggcac
    18541 tcccctcact cactctgtct gcgttgattg acgtaaaaaa accgccgaaa gaggcggcgt
    18601 ttactcgcgt atcccgaaaa cgtcagttca tgatttatgg cggaagtgcc attctggcca
    18661 tactgctctg gaacgggatc accatgtacc aggaataccg ggaaaaagaa gctgcggcag
    18721 aagctgcacg tcttcgtctg gcaaaagaaa tggcagacaa acaggcaata cagattgccc
    18781 caccgtggca acatttacct gaaataaaac catttattga taaatgcatt gataaatggg
    18841 atgcactgcc cttatcgatc gccggatggc gttttgacct tgctgaatgc agcacatccg
    18901 gtaatgacgg gctgttacgc acatcatata aggaactgag cggcgttacg gtcgaggact
    18961 tcagcacacg gatacgagaa atattccagg ggacaaccac agcaactttt gtattgccag
    19021 aaggcagcgc cgggggattt tctcttcctg tcagttttga cgtttcgcct gatccaatta
    19081 ccccggacac attgccacag gcaacagata ttcaggaacg cctgacaaca tttgcacaaa
    19141 aaatgaggtt aaaactcacc tggcaggaaa ttgaaaacac aaaaacagat gaagaaggcc
    19201 gtccaataat tctcccgtgg aacgagtatg aattaatgat tcagaccagc accccgcctt
    19261 caatactttt cgctaacttt catgaacctg ccgttcgctt ccagtatgca ggaataaaac
    19321 tggaagaagg aagactgaat tatgagatta aaggtgcatt ctatgttaaa aataattaaa
    19381 ccaacgatta tttctgtctg ttttttcagt tcacttgctt acgcaacaga atctgaaagc
    19441 acgccaaaag acgaaataaa atattcatct ccgaccattt tacaggtaga gcagattcag
    19501 gctgaaacgg taatttacga agcaaagctg gcgcgtcaga aggctgtcag tgaattgcgt
    19561 aatcttgatt tgaatggtgt agcgtccgca acgccagcaa taacgccctc ttccgggtat
    19621 tcttctcttg cagataccga tataccagcg caacgtgtag ccgctaaatc acttcgtgtt
    19681 attgaaatat ttggtacacc acaacaaatg tcagcacgaa tttctctcct ggacgggtca
    19741 gtaactgatg tcaaggtcgg gcaaagtatt cctggtactt cttataaggt ggcatcaatc
    19801 actggtaatg gcgtctggat tcagaatggt acagagaaac gccagcttat tctttaatgc
    19861 tattgaaaga gacgacacat aatgtttttt tctgacgcag actatcccgg aaaagatatt
    19921 tctgatgttg tatacgtcag tatatctgaa aaagacgata aacctgttca ggtttatatc
    19981 gctgataaca gtaaaggaca gcgtgctgtt caggcttata ttggcactct gaatgttaaa
    20041 tatcccggac aggtaaatat aacctcgaca actcttgatg ttatcgcatc ccgctaccag
    20101 gacagcgaaa aaaggtctga ttcaaactca tcacaacact taaacagcaa ccaggaaaag
    20161 gtcattagct acctggcgaa agcaaacaaa ctgggaagtt ccgatttgca catcacacct
    20221 ggtcgggacg gctccgagtt cacttatgtc gaagcacgcg tgcatgggga actggaaatt
    20281 ctggatgtca tcccgcgtaa agaagggctg gaattgctgg gtgctgccta ttctggaatg
    20341 agtgatgtta tcaaaggtac gcagtttgac cctgcaatac cacaggatgc acgtatcgca
    20401 gagaattttc tgaagcctgt aaatcttttt ggggcgcgct attcgcatta tccgtgcgtg
    20461 ggtggtgtgt acgcggtctt ccgcctgatt aaagatgaca gcgaggatat tcccactttc
    20521 gaagaacttg gctacatgcc gcaacaaatc cagacaatca ggcgaatgtt acaacgcccg
    20581 gaaggaatta tcgttttatc cggccctaca gggtccggta agtcaacgac tctgagaacg
    20641 gcttcagaag cctatctgag cacgtttggt ttcaaccata acgataatat gcgtctcccc
    20701 cgaaaacgcc ttttcaccat tgaatcaccg ccggagggcc gtattcccgg cgcaatacag
    20761 acggcagtca gggattccgt cgatggctgg gttgacgcga ttaaatccgc tatgcgactt
    20821 gatccagacg cgatactgaa cggcgaaatt cgtgatcacg catcggcatt agcagcaatc
    20881 aaagcctcga tgacaggcca ccttctgttg acgaccttac acgccaacga tgccctgaat
    20941 attattgagc gccttgaaat ggagaatatt caggccaggc tgattgcaga tcctcttctg
    21001 tttatcggtc tgctgagtca acggctggta caaaaactct gtccttactg taagcgcact
    21061 tatgctgaaa tggcagacaa actgagtaca gaagaacgga aactcattga ggaaaattgc
    21121 ctgccggaac aggtccggtt gcgcaatctt gacggttgcg agcactgtta taaaggcgta
    21181 acgggaagga cggttatagc agaagtcgtc tcacccgacg cacgtttttt tcagttatat
    21241 cgtcagtccg ggcgtctgga agcaaaatca tactggcact atgaacttgg aggaattacc
    21301 cgtaatcagc acttattaca cctgattaat catggcctgg tagatccgct gtccgcacac
    21361 ttcatatccc ccattgacga agataaatac tcaatgctcc cggaggggac atggcgataa
    21421 aagatatatt tgagaaactc aattcgatag aaattgattt ctctgggata tcccactggc
    21481 tggcaaaata cacattcagg tattccagaa taagtttata tgaagattta tctggaatgt
    21541 taaatgatgg cataaatgta aagcgtgctc ttacacacct gattgatgta gaaacagact
    21601 acgggaagaa aacggggtct tctgcaacct actatatttg ttgtgagtgc cttaatgcac
    21661 tgaataacac aggggcaata tcttcagcat taaaaaaata tataaaggag gatgaatatc
    21721 aattaatatc cagcggtgaa attcgcggcg accttgccgg gggattagtt caggccataa
    21781 aaattgtcag aagtcgttct gaaatgacaa tgactcttgt tatgtcgatg atttatccat
    21841 ccgtactggt ttttggttgt ctggcaaaca tgtatatggt ccacgatacc ataacacctg
    21901 ttttcctttc tctcgcaccg aaagaacgct ggacaagttc tatgcgatta ctgacagata
    21961 cgtctggttt cctgatagaa aacggaacgt atattctctg cttctgcatc gtactgtttt
    22021 tcctgatcag atattcgctt gcacgttata caggaccggg ccgtcagtat cttgattaca
    22081 ttcccccctg gtcactttat aaaacattta atggtgtaag ttttctcttc aacatggcct
    22141 ccatgttaag tattgatatt cccgttgcaa aagcactgga aaggctggag aaaaacagca
    22201 ctcaaaacag atggctactc gaacgaataa aagaaatcag gagatatgtt ttacttggac
    22261 agtcacttgc aatggcaatg aaaagttgcg gttatgactt cccatcgaaa ttatgcatta
    22321 acaaactatt acttcattct gaaaatgctg acaatgcatt gatgatgtct aactatgctg
    22381 ataaatggct ggaggaagca aaaggaaaag tcaaatcaac aggagtctgg ataactgtag
    22441 tatccgcatt aattgttttc gcatttattg taatgatgat tactgctctc tacgatgtca
    22501 gtaacgtcct gcaacactaa tccctaatat ttttaacctc tcaataaata aggaattatt
    22561 tattatgtct tctattaata ttttaaatat gcgttctgtt ttttcttctc tttctgcacg
    22621 ccgtaaaaaa gaacaggaca aaggggcaac actgatggaa gtgttattgg ttgttggtgt
    22681 tattgttgta cttgcagcaa gtgcatataa actctattca atggtgcaat ccaacattca
    22741 atcatccaat gaacaaaaca atgtcctgac agtaattgcc aacatgaaat cactgaaatt
    22801 ccaggggcgt tatactgaca gtaactatat taagactctc tacgcgcaag gattactgcc
    22861 atccgatatg atcgcagata caactggcgc aagcgcgaaa aacccgtggg gcggctccgt
    22921 tacaatcaca acctcttcgg ataaatactc cttcaatgtt gttgaggcca gcgtaccgaa
    22981 gaaaaactgc atggcaatgg taaacgccct gcgttcttcc agcgcaatct ccaagattaa
    23041 caacacttcc acatcaactg tcgatgcggc aactgtttgc tcatctgaca gcaatacgct
    23101 gaccttcagc actgattcct gatttttcat gtgcatcgga taagcgcctt cgggcgctta
    23161 ttttttccat gtttacagga ggagtattgt gcgttctgtt ctttatatcc tgctatcatg
    23221 tatttcatca ttaatctttt cattgcaacc cgcatattca gcgacaacga cccgatttga
    23281 tgcctgcttt gcacaagccg ggcaacgtta tctgattgat cccctgcttc tgaaaagcat
    23341 tgccactgta gaaagttcac tcataccaga ccggataaat cacaacaaag aaaaaggaaa
    23401 aatattaagc accgattacg gactgatgca aattaattca tcacacatcc ccaacctgaa
    23461 aaaacttggc gtaataaaag acaaatcaga gcttattgat aacccctgtc tcaatataca
    23521 aattggcgcg tggattctgg caacacattt tcagaaatgt ggcataaact ggagttgtct
    23581 tggcagttat aacgctggtt tcaaagaaag taacgaacag aaaagaatca aatacgcacg
    23641 ctatgtctac aataaataca tggtaaggta acttatgcta aacagcatat ttaatacggc
    23701 gatactttgc tgtgcaaata tcgtctgtca atgctacgcc tacaatgaag taaaattccg
    23761 tttaaataaa cttaatgtat cttataaaac cggcacagag aaatattact gtctgatact
    23821 gacaacgctt gccgttctgt atctttcatt aaaccagcta tcactgattc aatctataat
    23881 agtgattgtt ttctatgctt tccttacatt aatggcttgt atagaccttc tttcatttct
    23941 actaccacgc ctttataccg tgacatttat cttctctggc ctgttatatc agacatggaa
    24001 taacaatata ttgtctggtt tgttttgtgc aatgctgatg ttcttcatta tgctgtttgt
    24061 aagactttac tttgcatata aaaacggaac ggaaagtttc ggcatgggag atgtgctact
    24121 tattgcagga actggcgtat ggtttccaac gcctgagatt gcctgttcaa ttgtatttat
    24181 tgccgttatc ggcggaatta ttttcttcac tcttggcgga ttaaacaagc aaaaaaaata
    24241 tattccattt ggtccgttcc tttgtggcgg aatgtttgtt tactcactcg taccgggaat
    24301 attattctga ggattaagag caatgaaaaa gacagataaa ggtgtatctc tccttgaggt
    24361 attacttgtc atcggcataa tggtgatggt tatccctaaa gtttatgaaa acattgaaaa
    24421 ccatctgaat aatgttcgct ggcagaatgc agcagaacac gcaaatacct ataacaccgc
    24481 tgttcgtaat tatgttgctg acaatgcatc aacactactt gctggctctt tacccaaaac
    24541 aatcacgcca gcaacactca ttcagaaagg ctatctgaaa tctggttttt ctgaatccaa
    24601 ttttggacaa agctacatta ccggtattgc aaagaacagc aaaacatccc gtctggaagc
    24661 cctgacctgt agtaatggcg gtcagtccct gtcagaagcc ggaatgcgct cggtagcaag
    24721 catgattgaa gggcttggcg gctacatcaa cagcagcaag caggcgattg gcgcgggtgg
    24781 cggctggtct gacacgcctt caaattacgg gctgaactgc gcgaccgggc atattgcaat
    24841 ggcacttgtt ggcgctgatt tgcaggaaag cgaccgttta tatcgttact ccataaccaa
    24901 tcgtcccgac cttaacagga tgcatacggc gattgatatg aacagcaaca atctgaacaa
    24961 cgtcggcact ttaaacggca acgccgccgc gctctccggc gatatcagcg cacgtaacgg
    25021 tacgttctct ggcgcaattt caggaaatac agccacaacc aacggcgata tcacttcgaa
    25081 taatggctgg ctggtcacaa aaaacagcaa aggctggatg aacagtacct atggtggcgg
    25141 ctggtatatg tccgattcgt cctggttgcg cagcgtgaat aataaaggga tttatactgg
    25201 cggccaggta aaaggtggaa ctgtacgcgc tgatggacgc ctctatacag gcgagtattt
    25261 acagcttgaa aaaacagcaa cggctggcac ttcctgctcc cctaacgggc ttgtaggccg
    25321 cgacagtaca ggggcg
//
LOCUS       DAZFJL010000067        25089 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFJL010000067 DAZFJL010000000
VERSION     DAZFJL010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.067"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(1..798)
                     /locus_tag="ACHJ8C_002618"
                     /pseudo
     CDS             complement(1..798)
                     /locus_tag="ACHJ8C_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            1322..3250
                     /gene="thrS"
                     /locus_tag="ACHJ8C_002619"
     CDS             1322..3250
                     /gene="thrS"
                     /locus_tag="ACHJ8C_002619"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO5552459.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            3254..3796
                     /gene="infC"
                     /locus_tag="ACHJ8C_002620"
     CDS             3254..3796
                     /gene="infC"
                     /locus_tag="ACHJ8C_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO5552460.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3893..4090
                     /gene="rpmI"
                     /locus_tag="ACHJ8C_002621"
     CDS             3893..4090
                     /gene="rpmI"
                     /locus_tag="ACHJ8C_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO5552461.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            4143..4499
                     /gene="rplT"
                     /locus_tag="ACHJ8C_002622"
     CDS             4143..4499
                     /gene="rplT"
                     /locus_tag="ACHJ8C_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO5552462.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4616..4740
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4622..4666
                     /gene="pheM"
                     /locus_tag="ACHJ8C_002623"
     CDS             4622..4666
                     /gene="pheM"
                     /locus_tag="ACHJ8C_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO5552463.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4950..5933
                     /gene="pheS"
                     /locus_tag="ACHJ8C_002624"
     CDS             4950..5933
                     /gene="pheS"
                     /locus_tag="ACHJ8C_002624"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO5552464.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5948..8335
                     /gene="pheT"
                     /locus_tag="ACHJ8C_002625"
     CDS             5948..8335
                     /gene="pheT"
                     /locus_tag="ACHJ8C_002625"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO5552465.1"
                     /translation="MKFSELWLREWVNPAIDSDGLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGDDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPIAVEAPDACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARIYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8340..8639
                     /gene="ihfA"
                     /locus_tag="ACHJ8C_002626"
     CDS             8340..8639
                     /gene="ihfA"
                     /locus_tag="ACHJ8C_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO5552466.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8740..9720
                     /gene="btuC"
                     /locus_tag="ACHJ8C_002627"
     CDS             8740..9720
                     /gene="btuC"
                     /locus_tag="ACHJ8C_002627"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO5552467.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGELWILPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISAQQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9782..10333
                     /gene="btuE"
                     /locus_tag="ACHJ8C_002628"
     CDS             9782..10333
                     /gene="btuE"
                     /locus_tag="ACHJ8C_002628"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO5552468.1"
                     /translation="MQDSILTTVVKDIDGKVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            10333..11082
                     /gene="btuD"
                     /locus_tag="ACHJ8C_002629"
     CDS             10333..11082
                     /gene="btuD"
                     /locus_tag="ACHJ8C_002629"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO5552469.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPANLAQAYGMNFRRLDIEGHRMLISTI"
     gene            11160..11624
                     /gene="nlpC"
                     /locus_tag="ACHJ8C_002630"
     CDS             11160..11624
                     /gene="nlpC"
                     /locus_tag="ACHJ8C_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO5552470.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQAAIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11871..12584
                     /gene="ydiV"
                     /locus_tag="ACHJ8C_002631"
     CDS             11871..12584
                     /gene="ydiV"
                     /locus_tag="ACHJ8C_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO5552471.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTVEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNFAG
                     ELLKYPFIELLINENYPHFNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQIIPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12647..14083
                     /gene="selO"
                     /locus_tag="ACHJ8C_002632"
     CDS             12647..14083
                     /gene="selO"
                     /locus_tag="ACHJ8C_002632"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO5552472.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVESGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLDDEEDKYRLW
                     FTDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDGLNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRVRLQQDEVTDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(14087..14278)
                     /gene="hemP"
                     /locus_tag="ACHJ8C_002633"
     CDS             complement(14087..14278)
                     /gene="hemP"
                     /locus_tag="ACHJ8C_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO5552473.1"
                     /translation="MRYTDSRKLTPETDASHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(14410..15456)
                     /gene="aroH"
                     /locus_tag="ACHJ8C_002634"
     CDS             complement(14410..15456)
                     /gene="aroH"
                     /locus_tag="ACHJ8C_002634"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO5552474.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQPRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(15613..16446)
                     /gene="ppsR"
                     /locus_tag="ACHJ8C_002635"
     CDS             complement(15613..16446)
                     /gene="ppsR"
                     /locus_tag="ACHJ8C_002635"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO5552475.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16779..19157
                     /gene="ppsA"
                     /locus_tag="ACHJ8C_002636"
     CDS             16779..19157
                     /gene="ppsA"
                     /locus_tag="ACHJ8C_002636"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO5552476.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(19214..20860)
                     /gene="fadK"
                     /locus_tag="ACHJ8C_002637"
     CDS             complement(19214..20860)
                     /gene="fadK"
                     /locus_tag="ACHJ8C_002637"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO5552477.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQILADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLPRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTVSGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(20917..21210)
                     /gene="ydiT"
                     /locus_tag="ACHJ8C_002638"
     CDS             complement(20917..21210)
                     /gene="ydiT"
                     /locus_tag="ACHJ8C_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO5552478.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(21207..22496)
                     /gene="ydiS"
                     /locus_tag="ACHJ8C_002639"
     CDS             complement(21207..22496)
                     /gene="ydiS"
                     /locus_tag="ACHJ8C_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5552479.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPIERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVSASNPHHYAVGVKEVIGLTPEQINDRFNVTGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22551..23489)
                     /gene="ydiR"
                     /locus_tag="ACHJ8C_002640"
     CDS             complement(22551..23489)
                     /gene="ydiR"
                     /locus_tag="ACHJ8C_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO5552480.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTEQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDISH
                     QCPTETVPYVTPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23509..24273)
                     /gene="ydiQ"
                     /locus_tag="ACHJ8C_002641"
     CDS             complement(23509..24273)
                     /gene="ydiQ"
                     /locus_tag="ACHJ8C_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO5552481.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     TAVEKIGFDLLIFGEGSGDLYAQQVGLLVGELLQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24589..>25089
                     /locus_tag="ACHJ8C_002642"
     CDS             24589..>25089
                     /locus_tag="ACHJ8C_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFO5552482.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKN"
BASE COUNT         6217 a         6010 c         6592 g         6270 t
ORIGIN      
        1 tacttactga caaagtatga ttgtctatat ataaaatcgc ggacatatga ctgacattgt
       61 ttgccatcat tcgacagaag aaaatatcta aagcaaagcc aaaatcctga ctcgcgataa
      121 cgcgactcct tgcaagagga tggcgaagta atgctctgaa ttccgcgtcc cggactaagg
      181 agacatgctg ctgtatggat tcaacagagg agtaatgttt aatcaggttg actttgcctt
      241 ttccatttct caatgaatca agggcgtatt gtaatgacgg atggcgacaa aaaatagtgt
      301 cagaaggaga ttgcgaaaaa atgcactatc attgagattt actgttggct gtatggtttt
      361 ttctctatga atttttttgg acagtttcat gcaatggtta cgtttttcgg cagttaactg
      421 ttcctgttca atataatcca gtatgcttcg caaatggtta gctttaatag gcgtggtttc
      481 caggaataag gaaatgattt cttttttata gtgtaatgta atgagtgtat tttctgcatg
      541 gatgggtgaa gtggcaaagt taatgataaa aagggcagtg gaataacaaa tttgtgaaat
      601 ttttcgacag cttgcggtaa attcctgagt aacacagtat gacagcatcg aacttaaata
      661 agaggtatca agtagggtga tgagctggcg cgcaaattga ttaataggct ctgatgaagg
      721 cgttagtatg tcgctatcga tgtgcattct aagaggagac tcataatgag ttctgataat
      781 gatatcgttt tcactcataa tctactcgca ttatatatgt ctggaggttt tgagtatgat
      841 gattaattat tattgccaga acgaaatgtg cacgaaaaat tgctataata attgtgtcac
      901 ttttatcatt gcgatatgac cacttcgtca ctgaatctca tgatcgttta aataccacca
      961 ccaaataacg caacgaactt tgctggattc agtttatgct gctgtaaatc cgctcgtagt
     1021 aaacctttca gaagctctgt gatgttatca gtggttcttt aagcgttttg ctggtgtact
     1081 cactacaaac gaattgcgaa tcgaatcaat gtgaaacgga aaggtacaat ctcccttttt
     1141 ttagtgttaa catcgttcaa ccgggtggaa ggtgagaggt taatgtcttt catttgtgta
     1201 cctgattgcg aaccaattta gcatttgttg gctagatggt ttcgcaatga actgttaata
     1261 aacaaatttt tctttgtatg tgatctttcg tgtgggtcac cactgcaaat aaggatataa
     1321 aatgcctgtt ataactcttc ctgatggcag ccaacgccat tacgaccacg ctgtaagccc
     1381 catggatgtt gcgctggaca ttggtccagg tctggcgaaa gcctgtatcg cagggcgcgt
     1441 taatggtgaa ctggttgatg cttgcgatct gattgaaaac gatgcacaac tgtcgatcat
     1501 tactgccaaa gacgaagaag gtctggagat cattcgtcac tcctgtgcgc acctgttagg
     1561 gcacgcgatt aaacaacttt ggccgcatac caaaatggca atcggcccgg ttattgacaa
     1621 cggtttttat tacgacgttg atcttgaccg cacgttaacc caggaagatg tcgaagcact
     1681 cgagaagcgg atgcatgagc ttgctgagaa aaactacgac gtcattaaga agaaagtcag
     1741 ctggcatgaa gcgcgtgaaa ctttcgccaa ccgtggggag agctacaaag tctccattct
     1801 tgacgaaaac atcgcccatg atgacaagcc aggtctgtac ttccatgaag aatatgtcga
     1861 tatgtgccgc ggtccgcacg taccgaatat gcgtttctgc catcatttca aactaatgaa
     1921 aacggcaggg gcttactggc gtggcgacag caataacaaa atgttgcaac gtatttacgg
     1981 tacggcgtgg gcagacaaaa aagcgcttaa cgcttacctg caacgcctgg aagaagccgc
     2041 gaaacgcgac caccgtaaaa tcggtaaaca gctcgacctg taccatatgc aggaagaagc
     2101 gccgggtatg gtattctggc acaacgacgg ctggaccatc ttccgtgaac tggaagtgtt
     2161 tgttcgttct aaactgaaag agtaccagta tcaggaagtt aaaggtccgt tcatgatgga
     2221 ccgtgtcctg tgggaaaaaa ccggtcactg ggacaactac aaagatgcaa tgttcaccac
     2281 gtcttctgag aaccgtgaat actgcattaa gccgatgaac tgcccggggc acgtacaaat
     2341 tttcaaccag gggctgaagt cttatcgcga tctgccgctg cgtatggccg agtttggtag
     2401 ctgccaccgt aacgagccgt caggttcgct gcatggcctg atgcgcgtgc gtggttttac
     2461 ccaggatgac gcgcatatct tctgtactga agaacaaatt cgcgatgaag ttaacggatg
     2521 tatccgttta gtctatgata tgtacagcac ttttggcttc gagaagatcg tcgtcaaact
     2581 ctccactcgt cctgaaaaac gtattggcag cgacgaaatg tgggatcgtg ctgaggcgga
     2641 cctggcggtt gcgctggaag aaaacaacat cccgtttgaa tatcaactgg gtgaaggcgc
     2701 tttctacggt ccgaaaattg aatttaccct gtatgactgc ctcgatcgtg catggcagtg
     2761 cggtacagta cagctggact tctctttgcc ttctcgtctg agcgcttctt atgtaggcga
     2821 agacaatgaa cgtaaagtac cggtaatgat tcaccgcgca attctggggt cgatggaacg
     2881 tttcatcggt atcctgaccg aagaatttgc tggtttcttc ccgacctggc ttgcgccagt
     2941 tcaggttgtt atcatgaata ttaccgattc acagtctgaa tacgttaacg aattgacgca
     3001 aaaactatca aatgcgggca ttcgtgttaa agcagacttg agaaatgaga agattggctt
     3061 taaaatccgc gagcacactt tgcgtcgcgt cccatatatg ctggtctgtg gtgataaaga
     3121 ggtggaatca ggcaaagttg ccgttcgcac ccgccgtggt aaagacctgg gaagcatgga
     3181 cgtaaatgaa gtgatcgaga agctgcaaca agagattcgc agccgcagtc ttaaacaatt
     3241 ggaggaataa ggtattaaag gcggaaaacg agttcaaacg gcgcgcccta accgtatcaa
     3301 tggcgaaatt cgcgcccagg aagttcgctt aacaggtctg gaaggcgagc agcttggtat
     3361 tgtgagtctg agagaagctc tggagaaagc agaagaagcc ggagtagact tagtcgagat
     3421 cagccctaac gccgagccgc cggtttgtcg tataatggat tacggcaaat tcctctatga
     3481 aaagagcaag tcttctaagg aacagaagaa aaagcaaaaa gttatccagg ttaaggaaat
     3541 taaattccgt cctggtacag atgaaggcga ctatcaggta aaactccgca gcctgattcg
     3601 ctttctcgaa gagggtgata aagccaaaat cacgctgcgt ttccgcggtc gtgagatggc
     3661 gcaccagcaa atcggtatgg aagtgcttaa tcgcgtgaaa gacgatttgc aagaactggc
     3721 agtggtcgaa tccttcccaa cgaagatcga aggccgccag atgatcatgg tgctcgctcc
     3781 taagaagaaa cagtaaggcc ttcaagtagc actgtctgtg gagcctttgg gcttcacagg
     3841 cattgttcgc ctttgtttcg tttattaaca atgcgaagtg gaagttatta aaatgccaaa
     3901 aattaagacc gtacgcggtg ctgctaagcg cttcaaaaaa accggtaaag gtggttttaa
     3961 gcacaagcac gctaacctgc gtcacattct gaccaaaaaa gcgaccaaac gtaaacgtca
     4021 cctgcgtccg aaagccatgg tttccaaagg cgatctgggc ctggtaatcg cgtgcctgcc
     4081 gtacgcataa gccgttaacg tttttaactt tttaattaga atatagatac aggagagcac
     4141 atatggctcg cgtaaaacgt ggtgttattg cacgcgcacg tcacaagaaa attttgaaac
     4201 aagctaaagg ctactacggt gcgcgttctc gcgtataccg cgttgccttc caggctgtta
     4261 tcaaagctgg tcagtatgct taccgcgacc gtcgtcaacg taagcgtcag ttccgtcaac
     4321 tgtggattgc gcgtatcaac gcagcagcac gtcagaacgg tatttcttac agcaaattca
     4381 tcaatggcct gaaaaaagcc tctgttgaaa tcgaccgtaa gatcctggct gatatcgcag
     4441 tattcgacaa agtagcgttc accgctctgg ttgaaaaagc gaaagcagct ctggcataag
     4501 ccagttgaaa gagggagcta gtctccctct tttcgtttca acgccatcaa aacattgact
     4561 tttatcgccg tagccttttc aataaaggtc ttttgaagag taccaaaagg taacgcaagc
     4621 aatgaatgct gctattttcc gcttcttttt ttactttagc acctgactcc aggaggctag
     4681 cgcgtgagaa gagaaacgga aaacagcgcc tgaaagcctc ccagtggagg ctttttttgt
     4741 atgcgcgttt tgaaaaataa aaagcctgtc tcatcaggcg taattgtcgc agccagtctg
     4801 gacacggcca gcgcggagaa accggagcgt acttaagtac gtgaggattt cgagcactgc
     4861 ccgggaccaa aatggcaagt aaaatagcct gatgggatgg gctctaagtc caacgaacca
     4921 gtgtcactac tgacacaatg aggaaaaaca tgtcacatct cgcagaactg gttgccagtg
     4981 cgaaggcggc cattagccag gcgtcagatg ttgccgcgtt agataatgtg cgcgtcgaat
     5041 atttgggtaa aaaagggcac ttaacccttc agatgacgac cctgcgtgag ctgccgccag
     5101 aagagcgtcc ggcagctggt gcggttatca acgaagcgaa agagcaggtt cagcaggcgc
     5161 tgaatgcgcg taaagcggaa ctggaaagcg cagcactgaa tgcgcgtctg gcggcggaaa
     5221 cgattgatgt ctccctgccg ggtcgtcgca ttgaaaacgg tgggctgcat ccggtgaccc
     5281 gtaccatcga ccgtatcgaa agtttcttcg gtgagcttgg ctttaccgtg gcaaccgggc
     5341 cggaaatcga agacgattat cataacttcg atgctctgaa cattcctggt caccacccgg
     5401 cgcgcgctga ccacgacact ttctggtttg acgctacccg cctgctgcgt acccagacct
     5461 ctggcgtaca gatccgcacc atgaaagccc agcagccacc gattcgtatc atcgcgcctg
     5521 gccgcgttta tcgtaacgac tacgaccaga ctcacacgcc gatgttccat cagatggaag
     5581 gtctgattgt tgataccaac atcagcttta ccaacctgaa aggcacgctg cacgacttcc
     5641 tgcgtaactt ctttgaggaa gatttgcaga tccgcttccg tccttcctac ttcccgttta
     5701 ccgaaccttc tgcagaagtg gacgtcatgg ggaaaaacgg taaatggctg gaagtactgg
     5761 gctgcgggat ggtgcatccg aacgtgctgc gtaacgttgg catcgacccg gaagtttact
     5821 ctggtttcgc cttcggaatg gggatggagc gtctgaccat gttgcgttac ggcgtcaccg
     5881 acctgcgttc attcttcgaa aacgatctgc gtttcctcaa acagtttaaa taaggcagga
     5941 atagattatg aaattcagtg aactgtggtt acgcgaatgg gtgaacccgg cgattgatag
     6001 cgacgggctg gcaaatcaaa tcacgatggc gggcctggaa gttgatggtg tagaaccggt
     6061 tgccggtagc ttccacggcg tggtcgttgg tgaagtggtt gagtgtgcgc agcatccgaa
     6121 cgctgacaaa ctgcgtgtga caaaagtgaa tgtcggcgac gatcgcctgc tggacatcgt
     6181 ctgcggtgcg ccaaactgcc gtcagggcct gcgtgtggcg gtagcgacca ttggtgctgt
     6241 tctgccgggt gatttcaaaa ttaaagccgc gaaactgcgt ggcgaaccgt ctgaagggat
     6301 gctgtgctcc ttctctgagc tgggaatttc tgacgatcat aacggcatta tcgagctgcc
     6361 tgcggatgcg ccgattggca ccgacatccg cgaatacctg aaactcgatg acaacaccat
     6421 cgaaattagc gtgacgccaa accgtgccga ctgcttaggt atcattggtg ttgcgcgcga
     6481 cgttgccgtg ctgaaccagc tgccgctggt tgaaccggaa atcgttccgg ttggtgcgac
     6541 catcgacgac acgctgccga ttgcagtaga agcgccggat gcctgcccgc gctatcttgg
     6601 ccgtgtggta aaaggcatta acgttaaagc gccaactcca ctgtggatga aagaaaaact
     6661 gcgtcgttgc gggatccgtt ctatcgatgc agttgttgac gtcaccaact atgtgctgct
     6721 cgaactgggc cagccgatgc acgctttcga taaagatcgc attgaaggcg gcattgtggt
     6781 gcggatggca aaagagggtg aaacgctggt attgctcgat ggtactgaag cgaagctgaa
     6841 tgctgacacc ctggtcatcg ccgaccacaa caaggcgctg gcgatgggcg gcatctttgg
     6901 tggcgaacac tctggcgtga atgacgaaac gcaaaacgtc ctgctggaat gcgctttctt
     6961 cagcccgctg tctatcaccg gtcgtgctcg tcgtcatggc ctgcatactg atgcgtctca
     7021 ccgttatgag cgtggcgttg atccggcact gcagcacaaa gcgatggaac gtgcgacccg
     7081 tctgctgatc gacatatgcg gtggtgaagc gggtccggta attgatatca ctaacgaagc
     7141 aacgctgccg aagcgtgcaa ccatcacttt acgtcgtagc aaactggatc gcctgatcgg
     7201 ccatcatatt gctgatgagc aggtaacaga cattctgcgt cgtctcggct gcgaagtgac
     7261 cgaaggcaaa gacgagtggc aggcagttgc gccgagttgg cgtttcgaca tggagattga
     7321 agaagatctg gtcgaagaag tcgcgcgtat ttacggctac aacaacatcc cggatgagcc
     7381 ggtacaggca agcctgatta tggggactca ccgtgaagct gacctgtcgc tcaagcgcgt
     7441 aaaaacgctg ctcaacgaca aaggctatca ggaagtgatc acctatagct tcgttgatcc
     7501 gaaagtgcag cagatgatcc atcctggcgt tgaagcctta ctgctgccaa gcccgatctc
     7561 tgttgaaatg tcagcaatgc gtctttccct gtggaccggc ctgctggcaa ccgtggtgta
     7621 caaccagaac cgtcagcaga atcgtgtacg cattttcgaa agcggtctgc gcttcgtgcc
     7681 agatactcag gcaccgttgg gtattcgtca ggatctgatg ttagccggtg tgatttgcgg
     7741 taaccgttac gaagagcact ggaacctggc aaaagagacc gttgatttct atgatttgaa
     7801 aggcgatctt gaatccgttc tcgacctgac cggtaaactg aatgaggttg agttccgtgc
     7861 agaagcgaat ccggcactgc atccggggca atccgcagcg atttatctga aaggtgaacg
     7921 tattggtttt gttggggttg ttcatcctga actggaacgt aaactggatc tcaacggtcg
     7981 cactctggtg ttcgaactgg agtggaacaa gctcgcagac cgcgtggtgc ctcaggcgcg
     8041 cgagatttct cgcttcccgg cgaaccgtcg tgacatcgcg gtggtggtcg cagaaaacgt
     8101 tcccgcagcg gatattttat ccgaatgtaa gaaagttggc gtaaatcagg tagttggcgt
     8161 aaacttattt gacgtgtacc gcggtaaggg tgttgcggag gggtataaga gcctcgccat
     8221 aagcctgatc ctgcaagata ccagccgtac actcgaagaa gaggagattg ccgctaccgt
     8281 cgccaaatgt gtagaggcat taaaagagcg attccaggca tcattgaggg attgaaccta
     8341 tggcgcttac aaaagctgaa atgtcagaat atctgtttga taagcttggg cttagcaagc
     8401 gggatgccaa agaactggtt gaactgtttt tcgaagagat ccgtcgcgct ctggaaaacg
     8461 gcgaacaggt gaaactctct ggttttggta acttcgatct gcgtgataag aatcaacgcc
     8521 cgggacgtaa cccgaaaacg ggcgaggata ttcccattac agcacggcgc gtggtgacct
     8581 tcagacccgg gcagaagtta aaaagccggg tcgaaaacgc ttcgcccaaa gacgagtaat
     8641 ctgatctaac taaaaaggcc gctctgcggc cttttttctt ttcactgtcg aagagtcacc
     8701 gtaaaatcaa cgccatgaca cttcagcaga acggatacca tgctgacact tgcccgccaa
     8761 caacagcgac aaaatattcg ctggttatta tgcctgtcag ttttgatgct gctggcgctt
     8821 ctcttaagcc tttgcgccgg tgaactatgg attttgccag gtgactggtt ttctcctcgt
     8881 ggcgaactgt tcgtctggca aattcgcctg ccacgtacgc tggctgtatt gctggttggt
     8941 gcggcgctgg ctatatccgg cgctgtaatg caggcgttgt ttgaaaatcc tctggcagaa
     9001 cctggactac ttggcgtctc taacggcgca ggcgtggggc ttatcgccgc ggtattgctt
     9061 gggcaagggc aactccccaa ctgggcgcta gggctgtgtg cgattgctgg cgcgcttatc
     9121 atcactttaa tactcttacg tttcgctcgt cgtcatcttt cgaccagtcg gttattgctg
     9181 gctggcgttg cattagggat tatctgtagc gcactaatga cgtgggctat ctacttttcc
     9241 acctctgttg atttacgtca gctgatgtac tggatgatgg gcggttttgg cggcgtagac
     9301 tggcggcaaa gctggctgat gctggcattg atccccgtgt tgttgtggat ctgttgtcag
     9361 tccaggccga tgaatatgtt agcacttggc gagatctcgg cgcagcaact gggtttaccc
     9421 ctgtggttct ggcgcaatgt gctggtggca gcgaccggct ggatggttgg cgtcagtgtg
     9481 gcgctggcgg gtgctatcgg ctttattggt ctggtgatcc cacatattct ccggctgtgt
     9541 ggtttaaccg atcatcgcgt attacttccc ggctgcgcgc tggcaggggc gagcgcattg
     9601 ctgctggccg atattgtagc gcgcctggca ttagctgccg cagagctgcc aattggcgtg
     9661 gtcaccgcaa cgttgggtgc gccggtgttt atctggttat tgttaaaagc aggacgttag
     9721 ccgcaaaaga cggtctatga ttaaaagcta agatttttac tgaccacacc caggagaaac
     9781 gatgcaagat tccattctga cgaccgtagt gaaagatatt gacggtaaag tgaccacgct
     9841 ggagaagtac gccggtaatg tgctgttgat tgtcaatgtc gcctcaaagt gtggcttaac
     9901 gccgcaatat gagcagttgg agaatattca gaaagcctgg gctgatcgtg gttttgtggt
     9961 gctgggattc ccgtgtaacc agtttctgga gcaagaaccg ggtagcgatg aagagattaa
    10021 aacttattgc accaccacat ggggggtgac gttcccgatg ttcagtaaga ttgaagttaa
    10081 tggcgaagga cgtcatccgc tgtatcaaaa attgattgcc gcagcgccga ctgcagtcgc
    10141 gccggaagag agcggattct atgcccgaat ggtcagcaaa ggtcgtgcac cgctgtaccc
    10201 tgatgatatt ttatggaatt ttgagaaatt tttagttggc agggacggat tggtcatcca
    10261 gcgtttttcc ccggatatga cgccggaaga tcccattgtg atggaaagca ttaaactggc
    10321 gttggcaaaa taatgtctat tgtgatgcag ttacaggatg ttgcggaatc tacccgcctg
    10381 gggccgcttt ctggcgaggt tcgggctggg gagatcctgc acctggtggg gccgaatggc
    10441 gcgggtaaga gtaccttact ggcgcgaatg gccggaatga ccagcggtaa gggaagcatt
    10501 cagttcgcgg ggcaaccact ggaagcatgg tctgcaacaa agctcgcgct gcatcgcgcc
    10561 tatctttcac aacagcagac gccgccgttt gcaatgccgg tctggcacta cctgacactg
    10621 catcagcacg ataaaacgcg taccgaacta ctgaatgatg tcgcaggggc gctggctctt
    10681 gatgacaaac tcggacgtag caccaatcaa ctttccggcg gtgaatggca acgcgtacgt
    10741 cttgctgcgg tggtgctgca aatcacacca caagccaatc ccgcaggcca attgctgctt
    10801 cttgatgagc cgatgaacag tcttgatgtt gcgcaacaaa gtgccttaga caaaattctg
    10861 agcgcgctgt gtcagcaagg actggcgatt gtgatgagca gtcacgatct caaccacaca
    10921 ttgcgtcatg cgcatcgggc gtggttgcta aaaggtggaa aaatgctggc cagtggacgc
    10981 agagaagagg tgctcacgcc ggcaaatctg gcgcaggcct atgggatgaa ttttcgccgt
    11041 ctggatatcg aaggtcacag aatgctgatt tcgaccatct gatttcatta gtatcttgaa
    11101 aatcgggtaa ttacgacgct aaattaatgc aagaataaaa aacagaggaa ccgtacggaa
    11161 tgcgtttctg ccttattttg atcacagcac tgtttctggc cgggtgtagc caccataaag
    11221 caccgccgcc aaatgccaga ctttctgatt cgattaccgt tattgccggt ttgaacgacc
    11281 agctacaaag ctggcatggc acgccgtatc gttatggtgg catgacgcgg cgcggtgtgg
    11341 actgttcggg atttgtggtt gtgacgatgc gcgatcgttt cgatttgcag ctgccccgtg
    11401 aaaccaagca acaagccgct attggcacgc aaattgataa agacgagttg ctgccaggtg
    11461 acctggtctt tttcaaaacg ggttccggac aaaatggttt gcatgtaggt atttacgata
    11521 ccaacaacca atttatccac gcctctacca gcaagggagt gatgcgttcc tcacttgata
    11581 atgtctattg gcagaaaaat ttctggcagg cgagacgaat ctagtatgtc gaaaaatggg
    11641 cggcgatgat acatcgccgt ccatttactc ctgaatacag tagtattttg cagacaaaaa
    11701 ttccgtaaat ggattattcg tttataatca ggataatcta aaaaaaggac ccctgaaaaa
    11761 gtggttaaca tggaacaaat gaaagttact ccttctgtaa ttgttatgtc ttatggcctc
    11821 atccctttta gccggatact gaaaaacatc cttcgagagg gaaggttacc atgaagattt
    11881 ttttggagaa tctttatcat tctgattgct atttcctgcc gataagagat aaccagcaag
    11941 accttgtcgg cgttgagtta attacccatt tctccagtga agatggtacg gttcgtatcc
    12001 cgacgagtcg ggttatagcg caacttaccg tggagcaaca ttggcaatta ttttctgaac
    12061 agttggaatt actaaaatca tgccaacact tttttattca acataaactc tttgcctggc
    12121 ttaatttaac gccacaagtg gctacattat tattagacag ggataatttc gcaggcgaac
    12181 tattaaaata tccgtttatt gaactgttga ttaatgagaa ttacccgcat ttcaatgaag
    12241 gaaaagataa tcgcgatctt ttatccttat cgcaaatgta tccccttgtg ttaggtaatt
    12301 tgggagcagg taatagcaca atgaaagcgg tgtttgatgg tcttttcacc cgcgtcatgc
    12361 tggataaaag ttttattcag caacaaatca cgcatcgctc cttcgaaccg tttattcgtg
    12421 cgatccaggc gcaaatttcc ccttgttgca attgcatcat cgcggggggc attgatacgc
    12481 cagaaatatt ggcacagatt atcccctttg atttccatgc tctccaggga tgcctgtggc
    12541 ctgcggttcc tataaatcag ataacgacgt tggttcagcg ataaccctcc tgtttgctgg
    12601 tgtttaagac gagagtaacc gtctacacta tcaaacagga ggatctatga ccctgtcttt
    12661 tattacccgc tggcgcgatg aactgccaga aacctataca gcactttccc ctacgccttt
    12721 aaataatgcc cggctgattt ggcataatac cgaactggct aacacgctga gtattccatc
    12781 gtcgctgttt aaaaatggcg caggtgtctg gggcggcgaa accctactgc ctggtatgtc
    12841 accacttgcg caggtttaca gtggtcatca gtttggcgtc tgggcgggcc aactgggtga
    12901 tgggcgcggc attttactcg gcgaacaact gcttgctgat ggcactacaa tggactggca
    12961 tctgaaaggt gctggcctga cgccttattc gcgaatgggg gatggacggg cggttttacg
    13021 ttcgacgata cgagaaagtc tcgccagtga ggcgatgcat tatctgggca ttccgacgac
    13081 ccgcgcttta agtatcgtca ccagcgattc gccagtgtat cgggaaacgg tggagtcagg
    13141 cgcgatgctg atgcgtgtgg caccaagtca tctgcgcttt ggtcatttcg aacattttta
    13201 ctatcgccgc gagccggaaa aggttcgtca gttagctgac tttgccattc gtcattactg
    13261 gtcacatctt gatgatgagg aggacaaata ccgtctctgg tttaccgatg ttgtcgcacg
    13321 taccgcctcg ttaattgccc aatggcagac ggtcggcttt gctcatgggg tgatgaatac
    13381 cgacaacatg tcgctgctgg ggctgacgct tgattacggg ccgtttggtt ttcttgatga
    13441 ttacgaaccc ggttttattt gtaatcactc ggatcatcaa gggcgttaca gctttgataa
    13501 tcaacctgcc gtcgcgttgt ggaatttaca gcgtctggcg cagacattgt cgccatttgt
    13561 tgccgtagat ggcctgaatg aggccctgga cagctatcag caggttttgt tgacgcatta
    13621 tggacaacgg atgcggcaga aactgggctt catgacggag caaaaagagg ataacgcgct
    13681 acttaatgaa ttattcagtc tgatggcgcg agagcgcagc gattataccc gcacattccg
    13741 catgctgagt ctgaccgagc agcacagcgc ggcgtcaccg ctacgtgatg agtttatcga
    13801 tcgtgcggca tttgatgact ggtttgcccg ttatcgggta cgtttgcaac aagacgaggt
    13861 taccgatagt gagcgtcagc agctgatgca aagcgttaac ccggctctgg tgttgcgcaa
    13921 ctggttggcg caacgggcga ttgaagcggc agaaaagggt gatatgacgg aattgcaccg
    13981 cctgcatgag gcgttgcgaa atcctttcag cgacagagat gatgactatg tcagtcgtcc
    14041 acccgactgg ggtaaacggc tggaagtcag ctgctcgagt taaaggctac ttggtcaaca
    14101 gcagcttgcc agcctgagtt ttacggagca gatattcttg cccgtcatga tcgataatca
    14161 gtttgccatc cggacctaac agtgtctggc tggtaattcg ccggatgggc tgcggggaag
    14221 cggtcttgtg actggcatcc gtttcaggcg tgagttttct gctatccgta taacgcataa
    14281 aaattaataa taatttatat aacaatgatt cttattatca atattatggc gggccagagc
    14341 aagtagtaat ttgttgagaa gacaacaagt tgcgcagaaa tgcccatccc gtcaggaatg
    14401 ggcacgcatt cagaagcgag tatctaccgc agaggcgagt ttttcgacca ggcgttcggt
    14461 atcttcccag cccagacacg ggtcggtaat ggattgaccg taagtaagcg gctgaccgcc
    14521 gacgattttt tgcgttcctt cgcgcaggaa actttccgcc ataattccag caatcgccgt
    14581 agagccattg cggatttgct gacaaatatc ctcacaaact tctaactggc gacggtgctg
    14641 cttctggcag ttaccgtggc tgaaatccac caccagatgt tcaggtaaat caaactcgtg
    14701 cagcgtatcg caggctgcgg cgatatcatc ggcatgataa ttcggttttt tgccgccacg
    14761 cataataatg tggccatacg ggttgccgct ggtctgatag atggtcatct gaccgttttt
    14821 gtctggtgag aggaacatat ggctggcgcg ggctgcgcgg atagcatcca cggcaatccg
    14881 cgtgttgcca tcggtaccat ttttaaaacc caccggacag gagagtgccg aagccatttc
    14941 gcggtggatc tgactttcgg tagtacgtgc gccaatcgcg ccccaactga ttaaatcagc
    15001 aataaactga ccggtcacca tatcgaggaa ctcagtcgcg gtagggacgc ccagctcatt
    15061 tacctgtaaa agtaatttgc gcgccagctc cagaccgtga tttacccgat agctaccgtt
    15121 taaatctgga tcggagatga gtcctttcca gccgacaacc gttcgtggtt tttcaaaata
    15181 ggtgcgcatt acgatttcca gccgtggctg gtactggttg cgtagcgact gcagacgggt
    15241 ggcgtactcc attgcagcgg tgagatcgtg gatcgagcag gggccaataa tgaccaacag
    15301 tcgcttatct tcaccattca gtattttttc aattctacgg cgggagtcgg tgacatgggt
    15361 ggcgacgcca ggcgttacgg gataccgtag cgcgagttcg gcgggcgtta ccaggctctc
    15421 aatacgcgca gtacggagtt cgtcagttct gttcattaca agtctcagaa atttgttgct
    15481 tctacggcag gactaccggg aagtgatgtg catcacgata aaccaatcgc agctgatttc
    15541 aagtgcgagg tgtgtcacac ctcgccgggt ggttagcatg ataacaaaaa aataagctaa
    15601 tgcactagtt ctctagtaca ttcggcggct aaggcccatg atatcgagga tcttggtggc
    15661 aatctcttct accgaataat tggtactgtt aatccacggg atctgatttt tacggtacaa
    15721 cgcttctact tccgcgactt ccatcctgca ctgacgaagc gaggcatagc gactgttctc
    15781 ccgacgttcc tcgcgaatcg ccgccagacg ttccgggtcg atagtcaggc cgaacaattt
    15841 gtgctgaagt ggtttgagcg acgcgggtag caccagatta tccatatcgt cggcaataaa
    15901 ggggtaattt gccgcgcgga taccaaattg cattgccaga tacagactgg tgggggtttt
    15961 accacagcga gaaacaccga gcaggatcac ctgagcctgg tccagattgc gcaacgaaat
    16021 accgtcatcg tgggcaaggg tgtaatcaat cgccgcaatg cgcgcatcat atttattgag
    16081 attattaggg ttaaggccat gggtacgatg agcaatcggc gttggatcca gtttcatctc
    16141 ttgttgtagc ggggcaacca gcgcctgaac gatatcctgg caaaagcctt cactttgcaa
    16201 gatgatagcg cgaatctccg gcaacacgat ggagtagaag accagcgggc gcacacctgt
    16261 ctggtgataa attgcgtcaa tctgatcctt cactgcccgt gcacggctct cattttcgac
    16321 aaacggcagc gtaatgctgc tgatagtgac gggaaattgt gacattactg cgtgtcctaa
    16381 tacctccgca gttattgccg taccatcaga aatataaaaa acgtggcgat caacagcatt
    16441 atccattttg ttcttcccgt gatgcagaca taattctatg aaagcataaa ttaaaaacgc
    16501 acagaagcgt agaacgttat gtctggttta taaaatgaaa cttaaatttt attttttatg
    16561 aaaacagcat ttcattttta tggtttcgtt tataccgatg gtttatgtgg aaattgtcga
    16621 agagagcaga tttgcgcaac gctgggatca gtcttaaaaa gtaaaaaaat atatttgctt
    16681 gaacgattca ccgttttttt catctggtta aatatgcaaa gataaatgcg cagaaatgtg
    16741 tttctcaaac cgttcattta tcacaaaagg attgttcgat gtccaacaat ggctcgtcac
    16801 cgctggtgct ttggtataac caactcggca tgaatgatgt agacagggtt gggggcaaaa
    16861 atgcctccct gggtgaaatg attactaacc tttccggaat gggtgtttcc gttccgaatg
    16921 gtttcgccac aaccgccgac gcgtttaacc agtttctgga ccaaagcggc gtaaaccagc
    16981 gcatttatga actgctggat aaaacggata ttgacgatgt tacccagctt gcgaaagcgg
    17041 gcgcgcaaat ccgccagtgg attatcgaca ctcccttcca gcctgagctg gaaaacgcca
    17101 tccgcgaagc ctatgcacag ctttccgccg atgacgaaaa cgcttcgttt gcggtgcgtt
    17161 cctccgctac tgcagaagat atgccggacg cttcttttgc cggtcagcag gaaactttcc
    17221 tcaacgttca gggttttgac gccgttctcg tggcagtgaa gcatgtattt gcttctctgt
    17281 ttaacgatcg cgccatctct tatcgtgtgc accagggtta cgaccatcgt ggcgtagcgc
    17341 tctccgccgg tgttcagcgg atggtgcgct ccgacctcgc atcttctggc gtgatgttct
    17401 ccattgatac cgaatctggc tttgaccagg tggtgtttat cacttccgca tggggccttg
    17461 gtgaaatggt cgtgcagggt gcggttaacc cggatgagtt ttatgtgcat aaaccgacac
    17521 ttgcggcgaa tcgcccggct atcgtgcgcc gcaccatggg gtcgaaaaaa atccgcatgg
    17581 tttacgcgcc gacccaggag cacggcaagc aggttaaaat cgaagacgta ccgcaggaac
    17641 agcgtgacat cttctcgctg accaacgaag aagtgcagga actggcaaaa caggccgtac
    17701 aaattgagaa acactacggt cgcccgatgg atattgagtg ggcgaaagat ggccacaccg
    17761 gcaaactgtt cattgtgcag gcgcgtccgg aaaccgtgcg ctcacgcggt caggtcatgg
    17821 agcgttatac gctgcattca cagggtaaga ttatcgccga aggccgtgct atcggtcatc
    17881 gcatcggcgc gggtccggtg aaagtcatcc atgacatcag cgaaatgaac cgcatcgaac
    17941 ctggcgacgt gctggttact gacatgaccg acccggactg ggaaccgatc atgaagaaag
    18001 catctgccat cgtcaccaac cgtggcggtc gtacctgtca cgcggcgatc atcgctcgtg
    18061 aactcggcat tccggcggta gtgggctgtg gtgatgcaac agaacggatg aaagacggcg
    18121 agaacgtcac tgtttcttgt gccgaaggtg ataccggtta cgtctatgcg gagttgctgg
    18181 aatttagcgt gaaaagctcc agcgtagaaa cgatgccgga cctgccgttg aaggtgatga
    18241 tgaacgtcgg taacccggac cgtgctttcg acttcgcctg cctgccgaac gaaggcgtgg
    18301 gccttgcgcg tctggaattt atcatcaacc gtatgattgg cgtccaccca cgcgcactgc
    18361 ttgagtttga cgatcaggaa ccgcagttgc aaaacgaaat ccgcgagatg atgaaaggtt
    18421 ttgattctcc gcgtgaattt tacgttggtc gtctgactga agggatcgcg acgctggggg
    18481 ccgcgtttta tccgaagcgc gtcattgtcc gtctctctga ttttaaatcg aacgaatatg
    18541 ccaatctggt cggtggtgag cgttacgagc cagatgaaga gaacccgatg ctcggcttcc
    18601 gtggcgcggg ccgctatgtt tccgacagct tccgcgactg cttcgcgctg gagtgtgaag
    18661 cagtgaaacg tgtgcgcaac gacatggggc tgactaacgt tgagatcatg atcccgttcg
    18721 tgcgtaccgt tgatcaggcg aaagcggtgg ttgaggaact ggcgcgtcag gggctgaaac
    18781 gtggtgagaa cgggctgaaa atcatcatga tgtgtgaaat cccgtccaac gccttgctgg
    18841 cagagcagtt cctggaatat ttcgacggct tctcaattgg ctcaaacgac atgacgcagc
    18901 tggcgctcgg tctggatcgt gactccggcg tggtgtctga actgttcgat gagcgcaacg
    18961 atgcggtgaa agcactgctg tcgatggcga ttcgtgccgc gaagaaacag ggcaaatatg
    19021 tcgggatttg cggtcagggt ccatccgacc acgaagactt tgctgcatgg ttgatggaag
    19081 aggggatcga tagcctgtct ctgaacccgg acaccgtggt gcaaacctgg ttaagcctgg
    19141 ctgaactgaa gaaataaaat aaatacccgg cggcgttttg tcgccgggtt tatgtgatcc
    19201 ctgaagatga aacttattca atctcttcac agacatcctg agttaatcgc cgcataatat
    19261 ctttccttaa caaaaacttt tgtattttac ctgagacagt gcgcggtagt ttttcgatta
    19321 ccacgatatg ttcaggatat ttatatttcg cgacccgttt acggctaaaa aaagccacca
    19381 cctcttccag cgataatgaa tgatgcggcg ctttcagcac gacataagcg catgaacgtt
    19441 cacctaaacg ttcatcgggc atcgcaacca cacaggcatc gtgaatttta ggatgctgca
    19501 ataaaatatc ttctacttca cggctgctaa tattttcgcc gccgcggaca ataatatctt
    19561 tcttgcgccc ggttattttt atatagccag cctcatccat gcggcagaga tcgccgctgt
    19621 aataccagcc ttcttcatcc agggcacggg cggttaattc aggttcatca aaatacccca
    19681 taaacacatt ggggccacgc gaggcttctt caccttcgca acctggcggt aaagtcttgc
    19741 gtgcgtcatc gaccacttta atctctacac ctgcggcagc gtaaccgtcg gtgtgcataa
    19801 agcgcggcaa aggatcgtcg agattcacca ccgcatgcgg cgaactttct gtggaaccat
    19861 aaacacttaa taatttaatg ccgcgctgct ggcattctcg cgccactttt ttggggattg
    19921 tggtacctcc gcaaagaaag aagcgcagcg ctgaaaggtc tgcgggttgt ttctctagta
    19981 aattcaaaag atcatagaca aacggcgttg cgccgagcat acaggtgcaa cgctgctgct
    20041 caagcagcgc gagacacgca tcaggagtga aaatatctaa caacacgctg cgagcaccaa
    20101 tcaaaaaagg tgctgttacg ccatgcagaa agcccgttgc gtgaccaagt ggcgcaggca
    20161 tcataaagac atcctgccag gtcagattta gccgcgcgca ataagcccgc tcactggcga
    20221 gaatattgtt atgcgttagc atcacaccct ttggcagacc ctcggttccg gaggtaaata
    20281 gcacagcggc taattcatcg ccgtgggtcg ttatcgccgt ggtcagagga gtattgtcgg
    20341 cgagaatctg acttaatgag agggaagagg tggcgggagc cagtttgtcc acgccgacaa
    20401 tttgttgtag ttgtggaagc tgattttgca gcggcaggat taaatctacc ggacgcgttt
    20461 gtttaaacaa cgtcggtgca aagaacattt ttgcctgaca cttattgagc acccacacca
    20521 gttctgcttc ccgccaggaa ggtaacagcg gcacggaaac cgcaccggtt ttcaggcagg
    20581 caagatagat aacggtaaat tcacaccagc ctggcagttg aaatgcgatg cgatcgcctg
    20641 attcaatacc cttcgctaac atccagtttg ccagacagct cgcggcgtga tcgagcgcgc
    20701 tataggtgta cgttgcacca tgattatcga ccacggcaat tttgtctggc atcgcacgag
    20761 cggtctgctg ccagtaatcg gccagcgaag catcgcccca tagcccttgc tgacgatacg
    20821 ccgcacgacg ttgttcgtta aacgttaatg tcactttcat tttggactct cgagacagaa
    20881 catcaggccc gagatgcggg cctgtgggat gcatgattag ccgtagcgaa aatcaatacc
    20941 gaaggtgcct gcgggatatt gccactgttc gagaatagtg ttaccgcaca gcacccgaca
    21001 ggtgccgcac tccagacaac cggcagaatc aaaatgaatg ttgccggcat catcctgctt
    21061 ataaagtccg gcagggcagg ctttcattaa tttacggaat tcattgatat cgggatttgc
    21121 cgccagaatg atatgcgggt ggccctcatc aacatggaat ttattgacgc ctaatttgat
    21181 gtcgacgtta accgtagcgt tctggctcat agcgcggttg ctcccttaat gccatctttc
    21241 agcaagttga tcagcccaat tttcttcgcg tgtcccatga tcattttgcg taccggctgg
    21301 tttggtttgc cgtcaatggt gaacatctcg ttcatgatgt cggcgaccat tcgtgggtat
    21361 tggctaaaca ggcgcgggtt ttccatcagc gccgggatct tgcggaaatg ctgcatatcg
    21421 cgcataacac aactttgttc cagctcacgt ttgtattgcg ccagactgct ggcggagaaa
    21481 tccgcgcgtt ctttggcggc gatcaccgtt gtggcggcag cctgagctga tgcaatggct
    21541 aaatccatgc cgcgaactgt aaaacccaaa ttcaggcaga agcctgcggc gtcaccaacg
    21601 atcatcacgc catcgttaac cagctgcggc accattgcca gaccgccctc cggcaccata
    21661 tgcgcggaat attcaagcag tttgccgccg ctaatcagcg ggcgaatggc ggggtgttgt
    21721 ttaaaatctt ccagcatttg cggcacgctt ttttgcgcat gggcgatatc acccaatcca
    21781 caaaccagcc ccaacgatac ggaatccttg ttggtataga ggaatccgcc gcccatcagg
    21841 ccgtcagaag gggaaccggc aaacagccag gcggcacctt cctcgcccgt aacattaaag
    21901 cgatcgttga tctgttctgg tgtcaggcct ataacctctt taacaccaac agcgtaatga
    21961 tgcggattgg aagcggaaac cattcccagc gagcggccaa gcatcgagtt aacgccatca
    22021 gccagaatca ccacattcgc ttcgagaata tcatccccgg cctggacgcc agtgaccttg
    22081 tttccttcac gaaccagcgc atcgacgcgt acgcccggga taaactgcgc gcccgcctgc
    22141 tcggcttgtt ccatcaacca cgggtccaga cgattacgca atacggtata agacgcgtgt
    22201 tgcggaacat ctggttgctc gcggtgaaaa tcgagggtaa cggcgctttc ttcggttaag
    22261 aaggagattt tctcgcgtgt gaccttgcgt tctatcggcg ctgatgctgc aaagcctgga
    22321 ataattgctt cgagtgtgtg ggcataaaga cgcccgccgg tcatgttttt acatccggca
    22381 ctgtcgccgc gttctatcac cagtacatcc agcccggctc gcgccatgac cagtgcggca
    22441 acgctaccgg caacgcccgc accgaccaca atggcatcaa atttgtcatc cgacatgcgt
    22501 tatgctccct tcccgctgcg tttacagcgg gagatgtggt tgaaagggaa ttagcgactc
    22561 aactggctaa tcagggcagg gacgactttg tagatatcgc ccaccagacc gtagtcggca
    22621 tagttgaaga ttggcgcatt tttatcttta ttaatggcga caatcacttt tgcgccgttg
    22681 ccgccaacca tatgctggat ctgcccggag atccccagcg tcaggtagag atcggatttc
    22741 agcaacacgc cggagacacc gatataacgt tcacgctcca tccagttctc gccttcggca
    22801 attggacgtg aacagccgac ttcagcattc agcactgccg ccagttcgtg gaccattttc
    22861 aggtcatcct gcgccgccag accacgaccg acgccaacca cacgttttgc tttgctcagg
    22921 tccacgctgc ttgccgcttt cgcacggcgt tcgcgacaga gaatttcatg acgcggggta
    22981 acataaggta ccgtttctgt cgggcactga tgagagatat cactggtgca cggctcctga
    23041 acaccgggtg caagggtaat gatcgccagc gggctgttga tcttttcctg agcgaacgct
    23101 aacccgccat acatccggtg ttcggcgcaa atgtgaccat cgacaatatc caccgccgtg
    23161 gcatcgttca ccagcgccgc attcagttgc acacttaacc gtgctgccag agctttacca
    23221 cgtttcgtcg cggccaacag caacatagcg gggtgtttat ctttcagcag ggcagcaatg
    23281 ctttcggcgt aattttcagt acgttgcagc gcgtcgtttt gctcaagaac ataaatgcat
    23341 tttggcccat aaggcataac tgcctgcgcc tgctcggtat tttgtacaat ggcatacacc
    23401 tgttggcccc attgctgagc gccgccaaac agttcagcat aacgttcagg attatcgcta
    23461 aagacccaga cgctgtttaa ttgactcatc gtttcttctc cgtaggcttc agttcagggc
    23521 ttttttcaga tgttccgcca gctcggcaat ggcctccggc gaatcgttat cgagaatgat
    23581 gtgcttacgt tctgtttgcg gcggtacgcg aatgccggtc agttcagcaa gtggtgcgct
    23641 ctggctccag ccaatatcac ttgcctgcca ctgatttacc ggttttttac ccgcgccgag
    23701 gatggctttc atcgaaggaa tacgtggtac gttaatatcg gaggtgacgc agagcacggc
    23761 tggaacagag agttcaataa cttcaacatc atcttcaagc gtgcgttcaa tcaccagtgt
    23821 attgccctga cgctgaatag cactcactgc attaatcacc ggaagttgca gaagttctcc
    23881 gaccagcaag ccaacctgct gggcataaag gtcgccggaa ccttcaccaa agatcagtaa
    23941 atcgaagccg atcttttcaa ctgctgtcgc cagcgccttt gcggtatcga gaggcagtgc
    24001 atgctcaagt tgtgcatcct gcaccatata caggctgtgc ggcccgcggg atagcacgtc
    24061 tttgcgcact ttcgagttct gcaacaaaga gccgccgacg gtcagcgcgg ctatctcatc
    24121 gtcatctgtt gcaagctggc ttgcagcttc aatggcattg agatcgaact ggctgatttt
    24181 ggcgtcggca ttgtcgaaat tcagggtgta ttctggagtg acaacaatgt cctgttcttc
    24241 aggcaccagc ttaaagcagg ttattatttt catgatttct cctgtgagtc gcatacggaa
    24301 ccatctccga tggaaggtaa aaatggcctg aaaatagtgt gcgatagcgg tgcggggttt
    24361 tcgacggagc aagctgtttt ccttgccaac aatcttgcaa aaaaaatgcg atcgctaaaa
    24421 caaaagtgca atttgcggca atatagttat tccatgaccg tgcaatcttg cattcagtgt
    24481 aaataatctt gtttagctct cgctaaacgc ggttgtataa ccgcaataca cgccgtattt
    24541 tatccggcat atattgtttt tgtttgctca tggaataggg aggttgtcat gtatcaacgc
    24601 tgttttgata atgccagtga aacgctgttt gtcgccggta aaacgccacg gctttcacgt
    24661 ttcgcattta gcgatgatcc aaaatgggag tctgggcatc acgttcatga caatgaaacc
    24721 gagctgattt acgtcaagaa aggggtagca aggtttacca tcgattcttc gttatatgtc
    24781 gcgcatgccg atgacattgt ggtgatagaa cgtggcagac tgcatgcggt ggcctctgac
    24841 gttaacgatc cggcaacgac gtgtacctgt gcgctgtacg gctttcagtt tcagggggct
    24901 gaggaaaatc agctactgca accgcattct tgcccggtaa ttgccgcagg gcagggaaaa
    24961 gaagtcatta aaaccttatt taatgagcta agtgtgattt tgccgcaaag taaaaatagc
    25021 caaacatctt cgttatggga cgcatttgcc tatacgttag caattcttta ctacgaaaac
    25081 tttaaaaat
//
LOCUS       DAZFJL010000068        24909 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFJL010000068 DAZFJL010000000
VERSION     DAZFJL010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.068"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..952
                     /gene="ompT"
                     /locus_tag="ACHJ8C_002643"
     CDS             <1..952
                     /gene="ompT"
                     /locus_tag="ACHJ8C_002643"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO5552483.1"
                     /translation="RAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGKT
                     KERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVD
                     RDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYSF
                     TARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFK
                     YSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYIEGAWNRVT
                     NKKGDTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1184..1852
                     /locus_tag="ACHJ8C_002644"
     CDS             1184..1852
                     /locus_tag="ACHJ8C_002644"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5552484.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFDPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVKGTVVMFSA
                     SGKK"
     gene            complement(1907..2280)
                     /locus_tag="ACHJ8C_002645"
                     /pseudo
     CDS             complement(1907..2280)
                     /locus_tag="ACHJ8C_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2287..2888
                     /locus_tag="ACHJ8C_002646"
                     /pseudo
     CDS             2287..2888
                     /locus_tag="ACHJ8C_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(2904..2980)
                     /locus_tag="ACHJ8C_002647"
     tRNA            complement(2904..2980)
                     /locus_tag="ACHJ8C_002647"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2944..2946),aa:Arg,seq:tct)
     gene            3251..4117
                     /gene="folD"
                     /locus_tag="ACHJ8C_002648"
     CDS             3251..4117
                     /gene="folD"
                     /locus_tag="ACHJ8C_002648"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO5552485.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            4119..4331
                     /gene="ybcJ"
                     /locus_tag="ACHJ8C_002649"
     CDS             4119..4331
                     /gene="ybcJ"
                     /locus_tag="ACHJ8C_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO5552486.1"
                     /translation="MATFSLGKHPYVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            4439..4960
                     /gene="ybcI"
                     /locus_tag="ACHJ8C_002650"
     CDS             4439..4960
                     /gene="ybcI"
                     /locus_tag="ACHJ8C_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5552487.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKAIPPRLLFAGIVLAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(4996..6381)
                     /gene="cysS"
                     /locus_tag="ACHJ8C_002651"
     CDS             complement(4996..6381)
                     /gene="cysS"
                     /locus_tag="ACHJ8C_002651"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO5552488.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMNDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            6555..7049
                     /gene="ppiB"
                     /locus_tag="ACHJ8C_002652"
     CDS             6555..7049
                     /gene="ppiB"
                     /locus_tag="ACHJ8C_002652"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO5552489.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKDPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            7052..7774
                     /gene="lpxH"
                     /locus_tag="ACHJ8C_002653"
     CDS             7052..7774
                     /gene="lpxH"
                     /locus_tag="ACHJ8C_002653"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO5552490.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHHQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7892..8401
                     /gene="purE"
                     /locus_tag="ACHJ8C_002654"
     CDS             7892..8401
                     /gene="purE"
                     /locus_tag="ACHJ8C_002654"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO5552491.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNEWRKAQTDEVL
                     ENPDPRGAA"
     gene            8398..9465
                     /gene="purK"
                     /locus_tag="ACHJ8C_002655"
     CDS             8398..9465
                     /gene="purK"
                     /locus_tag="ACHJ8C_002655"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO5552492.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRAAETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELS
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDLNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(9888..10781)
                     /gene="ybcF"
                     /locus_tag="ACHJ8C_002656"
     CDS             complement(9888..10781)
                     /gene="ybcF"
                     /locus_tag="ACHJ8C_002656"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO5552493.1"
                     /translation="MKTLVVALGGNALLQRGEALTAKNQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEVVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRRATPDELAPFAKADGSMGPKVMAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(10778..11593)
                     /gene="ylbF"
                     /locus_tag="ACHJ8C_002657"
     CDS             complement(10778..11593)
                     /gene="ylbF"
                     /locus_tag="ACHJ8C_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO5552494.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQALAVAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAAAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(11604..12863)
                     /gene="ylbE"
                     /locus_tag="ACHJ8C_002658"
     CDS             complement(11604..12863)
                     /gene="ylbE"
                     /locus_tag="ACHJ8C_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO5552495.1"
                     /translation="MFTSVAQANAAVIEQIRRARSHWLDVQPASSLISELNKGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNTVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(12873..14540)
                     /gene="fdrA"
                     /locus_tag="ACHJ8C_002659"
     CDS             complement(12873..14540)
                     /gene="fdrA"
                     /locus_tag="ACHJ8C_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO5552496.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAVMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSASQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAETVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            14857..15906
                     /gene="allD"
                     /locus_tag="ACHJ8C_002660"
     CDS             14857..15906
                     /gene="allD"
                     /locus_tag="ACHJ8C_002660"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO5552497.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QKGVAVVGISRMGHSGAISYFVQQAARAGLIGISLCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVNALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNTITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            15928..17163
                     /gene="allC"
                     /locus_tag="ACHJ8C_002661"
     CDS             15928..17163
                     /gene="allC"
                     /locus_tag="ACHJ8C_002661"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO5552498.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDATVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            17174..17959
                     /gene="allE"
                     /locus_tag="ACHJ8C_002662"
     CDS             17174..17959
                     /gene="allE"
                     /locus_tag="ACHJ8C_002662"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO5552499.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFT
                     LSEGGYLYCPPGSLMTFVNVQAEDSQIFLYKRRYIPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(18088..19233)
                     /gene="glxK"
                     /locus_tag="ACHJ8C_002663"
     CDS             complement(18088..19233)
                     /gene="glxK"
                     /locus_tag="ACHJ8C_002663"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO5552500.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTIFPDAHYICLPIADG
                     GEGTVEAMVAATGGNIVKLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELILHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQP
                     LAANGGNLARVASIEMNECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQLTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(19255..20556)
                     /gene="ybbY"
                     /locus_tag="ACHJ8C_002664"
     CDS             complement(19255..20556)
                     /gene="ybbY"
                     /locus_tag="ACHJ8C_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFO5552501.1"
                     /translation="MFNFAVGRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(20612..21973)
                     /gene="allB"
                     /locus_tag="ACHJ8C_002665"
     CDS             complement(20612..21973)
                     /gene="allB"
                     /locus_tag="ACHJ8C_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO5552502.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKDGKIAAIGQDLGDAKDVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHVSSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(22033..23487)
                     /gene="ybbW"
                     /locus_tag="ACHJ8C_002666"
     CDS             complement(22033..23487)
                     /gene="ybbW"
                     /locus_tag="ACHJ8C_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO5552503.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPGG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKNIG"
     gene            complement(23657..24535)
                     /gene="glxR"
                     /locus_tag="ACHJ8C_002667"
     CDS             complement(23657..24535)
                     /gene="glxR"
                     /locus_tag="ACHJ8C_002667"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO5552504.1"
                     /translation="MTLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTDASDIIFIMVPDTPQVEDVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(24635..>24909)
                     /locus_tag="ACHJ8C_002668"
     CDS             complement(24635..>24909)
                     /locus_tag="ACHJ8C_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO5552505.1"
                     /translation="GRCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRG
                     EPGTGEINYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
BASE COUNT         6258 a         6678 c         6157 g         5816 t
ORIGIN      
        1 gcgggcgaaa cttctgggaa tagtcctgac aacccctatt gcgatcagct cttttgcttc
       61 taccgagact ttatcgttta ctcctgacaa cataaatgcg gacattagtc ttggaactct
      121 gagcggaaaa acaaaagagc gtgtttatct agccgaagaa ggaggccgaa aagtcagtca
      181 acttgactgg aaattcaata acgctgcaat tattaaaggg gcaattaatt gggatttgat
      241 gccccagata tctatcgggg ctgctggctg gacaactctc ggtagccgag gtggcaatat
      301 ggtcgatcgg gactggatgg attccagtaa ccccggaacc tggacggatg aaagtagaca
      361 ccctgataca caactcaatt atgccaacga atttgatctg aatatcaaag gctggctcct
      421 caacgaaccc aattaccgcc tgggactcat ggccggatat caggaaagcc gttatagctt
      481 tacagccaga ggtggttcct atatctacag ttctgaggag ggattcagag atgatatcgg
      541 ctccttcccg aatggagaaa gagcaatcgg ctacaaacaa cgttttaaaa tgccctacat
      601 tggcttgact ggaagttatc gttatgaaga ttttgagcta ggtggcacat ttaaatacag
      661 cggctgggtg gaagcatctg ataacgatga acactatgac ccaggaaaaa gaatcactta
      721 tcgcagtaaa gtcaaagacc aaaattacta ttctgttgca gtcaatgcag gttattacgt
      781 aacgcctaac gcaaaagttt atattgaagg cgcatggaat cgggttacga ataaaaaagg
      841 tgatacttca ctttatgatc acaatgataa cacttctgac tacagcaaaa atggtgcagg
      901 catagaaaac tataacttca tcactactgc tggtcttaag tacacctttt aacaacgtta
      961 aatagatttt ctccccggtg gtattgtata gccccgggga atttgtttac cgttaaaagc
     1021 aaacaatatc ccctaatctt gtaagcattt aacctgcgtc ctggcgtgta acctcttacc
     1081 gcaacacaag actcaatata ttcaaattcg ttatgccgtg gcatcattta accccttgta
     1141 attcatcgct ataattgatt taattcacaa ataaaactat aacatggtga aactaatgaa
     1201 aaaaaacaca gatgatgggg ctaaaattta cacaccactt accctaaagc tttatgactg
     1261 gtgggttttg ggagtatcaa atcggcttgc atggggatgt cctacaaagg aacaccttct
     1321 tccacacttt ctggaacatt taggtaacaa ccatctggat attggtgttg gaactgggtt
     1381 ttaccttact cacgtacctg agagtagtct gatatcttta atggatttga acgaagctag
     1441 cctgaacgcg gcatcgacaa gggctgggga atcaaaaatt aaacataaaa ttagccatga
     1501 tgtttttgat ccttatcccg cggcgttaca tggtcaattt gattccattt ccatgtttta
     1561 ccttcttcac tgcctgcctg gaaatatatc tacaaaaagc tgtgtaatac gcaatgcggc
     1621 gcaggcctta actgacgatg gaactctata cggagccaca attcttggtg acggcgttgt
     1681 gcacaatagc ttcggtcaaa aactgatgcg catttacaat cagaaaggca tcttttcaaa
     1741 cacaaaagat tccgaagaag gcttaacaca tatactctca gagcatttcg agaatgttaa
     1801 aaccaaggtt aaaggtactg tagtaatgtt ttccgcttca gggaaaaaat agcatccagc
     1861 cgcagcacgt tcttgcgcac gacgtgctgc ggcataatcc caatgattac tctctgacag
     1921 ggttcacagg ccactcaata tcaggggcag ttgatgtatc aactcggttc agcaacaccc
     1981 gatacatctt ccaggcttcc agcaacgagg tttcttcctc cgtatccttt atccatgata
     2041 tgccgttcca cttctgaaac tcccctgccg gggataccag gtaacatttt ccggtaacgg
     2101 accgagctca gaaataaata acgcgtcccc tgatgccacg tcataaaccg ttttaccccg
     2161 atggtcttca acgagatgcc acgatgcctc atcactgttg aaaacaacca cgaagccagc
     2221 cggaatatct ggcggtgcaa tatcagtact gtttgcaggc aaacctgtat gaggtggaat
     2281 ggcacaagag acttacaaaa gaatcaggaa aaacgcctga ttgatgagag ccccgagcca
     2341 ctgaaatctg tcgtcaagtt tgcgctggta actgttctga gaaagtcgaa catcataaat
     2401 ctggagtggc aacagattga catgcagcga caagttgcca gggtgaatcc agaagatagc
     2461 aaatcaaacc acgccattgg tgtggcgctg aacgataccg cctctaaagt gttgcgtgct
     2521 caaacaggca agcatcacaa atgtgggtgt acataccagg gcggctaagc gagcagatgg
     2581 aacatcaatg cctgcgctca ggaaaatgcg cattgacagc aagacagcat ggctatcagc
     2641 ttgtcgtcgt gcaggaattg aagatttccg tttccatgac ctctggcata ccagggcaag
     2701 caggttgatt ccgtcaggcg ccccattatc agtgcttcag gaaatgggcg gatgggagtc
     2761 cagagaaatg attcgtagat atgctcacct tgcgcctaat catttgactg agcacgcgag
     2821 gaaaatagac gacatttttg gtgataatgt cccactctgg aattatagaa gaaataaaga
     2881 aggcgtaact gattgaattt taatggcgcg ccctgcagga ttcgaacctg cggcccacga
     2941 cttagaaggt cgttgctcta tccaactgag ctaagggcgc attgatacca cggtgtgctg
     3001 taatcgcgtg aattatacgg tcaacccttg ctgagtcaat ggcttttgaa ccagttgctg
     3061 aacaagtgta cgaccgcatc tgatgtagct cactatggcg gcaaaccaac gacggttatc
     3121 atgataggtg tgaatcagcc aggtacagaa ggttttactt ttcagaaaac gaaaactgac
     3181 agcgcgccac gcttctgaca aaataggcgc atccccttcg acctacgtaa cagatggaat
     3241 cctctctctg atggcagcaa agattattga cggtaaaacg attgcgcagc aggtgcgctc
     3301 tgaagttgct caaaaagttc aggcgcgtat tgcagccgga ctgcgggcgc caggactggc
     3361 cgttgtgctg gtgggtagca accctgcatc gcaaatttat gtcgcaagca aacgcaaggc
     3421 ttgtgaagaa gtcgggttcg tctcccgctc ttatgacctc ccggaaacca ccagcgaagc
     3481 ggagctgctg gagcttatcg atgcgctgaa tgccgacaac accattgatg gcattctggt
     3541 tcaactgccg ttaccggcgg gtattgataa cgtcaaagtg ctggaacgta ttcatccgga
     3601 caaagacgtg gacggtttcc atccttacaa cgtcggtcgt ctgtgccagc gcgcgccacg
     3661 tctgcgtccc tgcaccccgc gcggtatcgt cacgctgctt gagcgttaca acattgatac
     3721 ttttggcctt aacgccgtgg tgattggcgc atcgaatatc gtcggtcgcc caatgagcat
     3781 ggaactgctg ctggcaggtt gcaccactac cgtgacccac cgtttcacta aaaatctgcg
     3841 tcaccacgta gaaaatgccg atctactgat cgttgccgtc ggcaagccgg gctttattcc
     3901 tggtgactgg atcaaagaag gcgcaattgt gattgatgtc ggcatcaacc gtctggaaaa
     3961 tggcaaagtt gtgggcgacg tcgtgtttga agacgcggct aaacgcgcct catacattac
     4021 gcctgttccg ggcggcgttg gcccgatgac ggttgccacg ctgattgaaa acacgctaca
     4081 ggcgtgcgtt gaatatcatg atccacaggg tgagtaacat ggcgacattt tctttaggta
     4141 aacatccgta cgttgagctg tgcgacttgc tgaaactgga aggctggagc gaaagcggcg
     4201 cgcaggcgaa aatcgcgatt gccgaaggcc aggtgaaagt cgacggtgcg gttgaaacgc
     4261 gcaaacgctg caaaattgtc gccggtcaga cagtgagttt tgcaggtcac agcgtacagg
     4321 ttgttgcctg atccctttcc cggcctgtat caacaggccg gttctctttt cagacttttg
     4381 ccctctacgc cccgttcatc catgctgata gcatcgacat tttgccgaag cgaccatcat
     4441 gccaaccgtt attacgcacg ccgccgttcc cctatgcatt ggtttagggt tgggttcgaa
     4501 agccatccca ccgcgtctgt tatttgccgg aatcgtcctg gcgatgttgc ctgatgccga
     4561 cgtattatcg tttaaatttg gcgttgctta cggcaacgtt tttggtcatc gcgggtttac
     4621 ccattcgctg gtgtttgcgt ttgttgtccc gctgttatgt gtgctgattg gacgtcgatg
     4681 gttcagggct gggctgattc gctgctggct gtttttaacc gtctcattgc tatcgcacag
     4741 cttgctggat tcggtaacca ctggtggtaa aggcgttggc tggctatggc cgtggtcaga
     4801 tgaacgcttt ttcgctccct ggcaggtgat taaagtcgcg ccgtttgcat tgtctcgtta
     4861 caccacgccg tacgggcatc aggtgattat ttctgaattg atgtgggtgt ggttgccggg
     4921 gatgctactg atgggaatgt tgtggtggcg caggcgataa ccggatgcga aaactcgcat
     4981 ccggcaatag cgcaattact tacgacgcca ggtggtccct tgcgggccat cttccagcac
     5041 gatccccatc tcgttaagac ggtcacgcgc cgcatctgcc gccgcccagt ctttcgcttt
     5101 acgggcatcc agacgctgtt gaattaacgc ttcaatctca gccacttcgc tgtcgtctgc
     5161 ctgcgcaccg ctttgcagga atgcttccgg ttcttgctcc agcagaccca atacggcaga
     5221 aagtttacgc aggtgagacg ccattgcatt cgccgcagcc atatcttctg ctttcagacg
     5281 gtttacttca cgcgccatat caaacagtac ggaataggct tccggggtgt tgaaatcgtc
     5341 gttcatcgcc tcgataaagc gcgcttcaaa cgcttcgcca ccggcaggag caacggtttt
     5401 gtctgtgccg cgcagtgcgg tgtagagacg ttccagcgcc gcacgcgcct gtttcaggtt
     5461 ctcttcactg tagttcaact gactgcgata gtgacccgac atcaggaagt aacgcacagt
     5521 ttccgcatcg tagtatttca gcacgtcacg cacggtgaag aagttaccca gcgatttaga
     5581 catcttctcg cggtcaacca tcaccatacc ggagtgcatc cagtaattca catactggcc
     5641 atcgtgcgca caggtggact gggcaatttc gttctcgtgg tgcgggaaca tcaggtcaga
     5701 accgccgccg tggatatcaa agtggttacc caactgtttg cagttcattg ccgaacactc
     5761 aatgtgccag ccaggacggc ccgcgcccca cggagacggc cagctcggtt cgccctcttt
     5821 cgacatcttc cacagaacga agtccatcgg gttgcgtttg tcgtcgacca cgtcaacgcg
     5881 cgcgcctgcc tgcagctgat cgagatcctg acgcgacagc acgccatatg tcggatcggt
     5941 cgggacgtcg aacatcacgt cgccgttgtc cgccacataa gcgtgacctt tggcgatcag
     6001 ttgttcagtg agttcaataa tttcggcgat atggtgcgtc gcgcgcggct ccatatccgg
     6061 gcgcaaaatg ttcaaagcat caaaatcttt gtgcatttcg gcgatcatgc gatccaccag
     6121 cgccacaaag ctttcgccat tttcattggc gcgtttgatg attttgtcgt cgatatcggt
     6181 aatgttgcgc acatacttca gcttatagcc gaggaaacgc agatagcgcg caaccacgtc
     6241 aaaggcaaca aaggtacgcc cgtgaccgat atgacagaga tcgtaaacgg tgattccaca
     6301 cacgtacatg ccgacttccc cggcgtgaat aggcttaaat tcctcttttt ggcgtgtcag
     6361 agtattgaag atttttagca tcgaagattc cgtttagaca tgtgtgggta attgagttgc
     6421 gtataatacc catatttccc gcgcgaatca gcatacattg cgagatgatc cgatcgtgcg
     6481 gttatgctat aacaccaccc tatatatgac ccgaaccggg ttgaagcacc aatcaaacgg
     6541 aacaggatgc aaaaatggtt actttccaca ccaatcacgg cgatattgtc atcaaaactt
     6601 ttgacgataa agcacctgaa acagttaaaa acttcctgga ctactgccgc gaaggttttt
     6661 acaacaacac cattttccac cgtgttatca acggctttat gattcagggc ggcggttttg
     6721 aaccgggcat gaaacaaaaa gccaccaaag acccgatcaa aaacgaagcg aacaacggtc
     6781 tgaaaaatac ccgtggtacg ctggcaatgg cccgtactca ggcaccgcac tctgcaactg
     6841 cccagttctt catcaacgtg gttgataacg acttcctgaa cttctctggc gaaagcctgc
     6901 aaggttgggg ctactgcgta tttgctgaag tggttgaagg catggacgtg gtagacaaaa
     6961 tcaaaggtgt ggcaaccggt cgtagcggta tgcaccagga cgtgccaaaa gaagacgtta
     7021 tcattgaaag cgtgaccgtt agcgagtaat cgtggcgaca ctctttattg cagatcttca
     7081 tctctgcgtg gaagaaccgg cgatcaccgc cggttttctg cgttttttag cgggggaggc
     7141 ccgcaaggcc gatgcgctgt atattcttgg cgatctgttt gaagcctgga tcggcgacga
     7201 cgatcccaac ccacttcatc accagatggc cgcagcgatc aaagcggtat ccgattccgg
     7261 cgttccctgt tatttcattc atggcaaccg tgattttctg ctcggcaaac gctttgcccg
     7321 tgaaagcggc atgacgttat tgccggaaga aaaggtgctc gaactttatg gtcgtcgggt
     7381 gttgattatg catggcgaca cgctgtgcac cgatgacgcg ggttatcagg cttttcgcgc
     7441 caaagtccac aaaccctggc tgcaaacgct atttctcgcc ctgccgttgt ttgtgcgcaa
     7501 acgcattgcc gcgcgaatgc gcgcgaacag caaagaagcc aacagcagta aatcgctggc
     7561 gatcatggac gttaaccaaa acgcggtggt cagtgcgatg gaaaaacatc aggtgcaatg
     7621 gctgattcac gggcataccc atcgcccggc agtacatgaa cttatcgcca atcagcaacc
     7681 tgctttccgc gtcgtgctgg gtgcctggca tacggaaggt tcaatggtga aagtcacggc
     7741 ggatgacgtt gagctgattc atttcccgtt ttaaaaaacc cgcaactttg ctgatttcac
     7801 agccacgcaa ccgttttcct tgctctcttt ccgtgctatt ctctgtgccc tctaaagccg
     7861 agagttgtgc accacaggag ttttaagacg catgtcttcc cgcaataatc cggcgcgtgt
     7921 cgccatcgtg atggggtcca aaagcgactg ggctaccatg cagttcgccg ccgaaatctt
     7981 cgaaatcctg aatgtcccgc accacgttga agtggtttct gctcaccgca cccccgataa
     8041 actgttcagc ttcgccgaaa gcgccgaaga gaacggttat caggtgatta ttgcgggcgc
     8101 aggcggcgca gcgcacctgc ctggcatgat tgccgccaaa acgctggtgc cggtgctggg
     8161 cgtgccagta cagagcgccg cactgagcgg tgtcgatagc ctctactcca tcgtacaaat
     8221 gccgcgcggc attccggtgg gtacgctggc gattggtaaa gctggcgcgg caaacgcggc
     8281 gttactggca gcacaaattc ttgcgactca tgataaagaa ctgcaccagc gtctgaatga
     8341 atggcgcaaa gcacaaactg atgaagtgct ggaaaacccg gacccgcgag gtgcggcatg
     8401 aaacaggttt gcgtcctcgg gaacgggcag ttaggccgta tgctacgtca ggcaggcgaa
     8461 ccgttaggca ttgctgtctg gccggtcggg ctggacgctg aaccggcggc ggtgcctttt
     8521 caacaaagcg tgattaccgc tgagatcgaa cgctggccgg aaaccgcatt aacccgcgag
     8581 ctggcacgcc atccggcctt tgtgaaccgc gatgtgttcc cgattattgc cgaccgtctg
     8641 actcagaagc agcttttcga taaactccac ctgccgaccg caccgtggca gttacttgcc
     8701 gaacgcagcg agtggcctgc ggtgtttgat cgtttaggtg agctggcgat tgttaagcgc
     8761 cgcactggtg gctatgacgg acgcggtcaa tggcgtttac gcgccgctga aaccgaacag
     8821 ttaccggctg aatgctacgg cgaatgtatt gtcgagcagg gcataaactt ctctggtgaa
     8881 gtgtcgctgg ttggcgcgcg cggttttgat ggcagcaccg tgttttatcc gctgacgcat
     8941 aacctgcatc aggacggcat tttgcgcacc agcgtcgctt tcccgcaggc caacgcacag
     9001 caacaggcgc aagctgaaga gatgttgtcg gcgattatgc aggagctgag ctatgtaggc
     9061 gtgatggcaa tggagtgttt tgtcacccca caaggtctgc tgatcaacga actggctccg
     9121 cgtgtgcata acagtgggca ctggacacaa aacggtgcca gcatcagcca gtttgagcta
     9181 catctgcggg caattaccga tctgccgtta ccgcaaccag tagtgaataa tccgtcggtg
     9241 atgatcaatc tgattggtag cgatctgaat tatgactggc tgaaactgcc gcttgtgcat
     9301 ctgcactggt acgacaaaga agtccgtccg ggccgtaaag tggggcattt gaatttgacc
     9361 gacagcgaca catcgcgtct gaccgcgacg ctggaagcct taatcccact gctgccgccg
     9421 gagtatgcca gcggcgtgat gtgggcgcag agtaagttca gttaattgat gctcattttc
     9481 ttgccgcatg cggcgtgaac gccttatcag gcctaccgtt ccaacaccga ctgtaggcct
     9541 gataagatgc gccagcatcg catcaggcat ggtgtgccga ttgccggatg cggcgtgaac
     9601 gccttatccg gcctaccgtt ccgacaccgc ctgtaggcct gataagatgc gccagcatcg
     9661 catcaggcat ggtgtgccga ttgccggatg cggcgtaaac gccttatccg gcctaccgtt
     9721 ccgacaccgc ctgtaggcct gataagatgc gccagcatcg catcaggcat gtgccggacg
     9781 cggcgtaaac gccttatccg gcccaccgct ccaacaccga ctgtaggcct gataagacgc
     9841 gccagcatcg catcaggctg gatgtgccaa ttgcctggtg cccatcacta cagcgaaata
     9901 caagtccccg cttcgcccgc cagcgtctct tcaatccgcg ataacgcccc aatccacgcg
     9961 ggtttaccac ggcttctgac atagccactc accgccataa ccttcggccc catcgaacca
    10021 tcggctttgg caaatggcgc taactcatcc ggtgtggcac ggcgaatggc acgttgctgc
    10081 ggcgttcccc agttttcata taccgcatca gcatcggtga ggatcaccag tccatcggca
    10141 ttaatctgct cggcgagcaa cgcagcggcg agatctttat caatcaccac ttcactccct
    10201 gccccgtctt ccgtcacagg cacaccgccg ccgccactgc aaatcaccac atgcccctct
    10261 ttgagcaaca actcgatggc ttcgctgtcg agaattttac gcggttgcgg agatgccacc
    10321 acccggcgca agtatttacc atcacgtttc atctgccagc cgtaagccgc ttccagtgcc
    10381 tcttgttctt ctggctgata aaccggaccg ataaatttct ctggctgcaa aaacgccgga
    10441 tcatcaggcg aaacttcaat gcgcgtcagc actgtcgtga caggcggcat ctgcggctgt
    10501 gcgctcaagc tctgcgccag catatagcca atcatcccct ggctttccgc caccagtaca
    10561 tccagcggat acggttctac ctctttccac gccagattct gtaacgccag cagccccacc
    10621 tgcggtccgt tgccgtgaac aatcgccagc cgataagaac gggccaggcg cgccagcgcg
    10681 ggtacagcac tggcaatatt gcgatattga ttttttgccg tcagcgcctc accgcgctgg
    10741 agtaaggcgt tgcccccaag agccacaacc agtgttttca tggttttcct tgtaataatt
    10801 gttggccaag ccagaacccc agcagcgtat cggcccctga cgtatgcccc agcgccagca
    10861 gcgaatcgat cgccgctgcg gtacgtttcg ggcaactcag agcatgaaca aagtgcagga
    10921 gtggcgaggc gaaatatcct tgcgcggcat aacgtaaata actgacgctc accgcagtgg
    10981 taacgagttg aagattgtcg gaacaggcaa aaaacgggcg accggagcgc gcatctaaag
    11041 ccccataata ccaggccgcc agcagcattc cgctcagcgt gtcatcatga cttggcgtta
    11101 atccggggcc tttacccagc cagtgccgcc agtcggtctt aacgccattg agcgcggcct
    11161 gaaaacagtg acgaaactgg cgtaattcag caggcagcgg atcgcttgcc gccaatgcca
    11221 gtggcccgaa aagcccggtt tcctccgcgc gttgcatcca tgcaacggca agcgcttgag
    11281 gatgcgcagg cggcgtaata cgtagcaaac aataacgctg tggctgttta accgtgaaac
    11341 gcccgaggcg aatcccttgt gccacaacct gtggtcgttc attgccgcat aatccaccgc
    11401 ataacgcatc gaattgcgcg cggcgaagca cccatccgcc ggggccgaaa ccactcccct
    11461 gacgatgcag cgttaacagt tcgccgcttt ccgtcatcag gttaatcgcc cgccgccaca
    11521 ctcccgctaa ccgccaggac tggcgataat tcggtgcgct acggctggca agtagaggat
    11581 ggatgatcgt cattgtgcgt tcctcaacca atccccatgc tttcagccag tgcttccagc
    11641 gcctgttcaa agcacgccag cggtgcccgc acggtgcctg cgccaatctg cccgatcccc
    11701 gcctctttat gggcgatacc ggtattgatg agtggcgtaa tgccggtttc taccacgcga
    11761 cgaatgtcca gccccaggca cgccccctgg aaatcccagc cggggatctg caattgcata
    11821 ttgcgttcaa ggtaaatttc cgccatctct tcagataccg ctctggccgc ttccatgcca
    11881 cccgcaccga caaagcgcgt tacgccaggc gctgcgatca ttgccgcacc tccgatacca
    11941 aaggtttcgg taatcgcgct atcgcccata tccgggttcg cctgctcctg cgagaagccg
    12001 gtgaaaaaca gaccttgcgg agtgtttaca ggcgcagtaa accagcgttc acccagcccg
    12061 cttacccgaa tcccgaacat attgccgttg cgggtcatag ccgtaacgat gctgcctgcg
    12121 cggatcatcg cgcctgcatc catcgccgcc ttgcagtagg ccatcgcgag attgaggaag
    12181 aactgatcgg tcacgctgag gaaatccatc acttccgcaa tgtgctgttt atcatgatcg
    12241 aggcgagcaa tttgtggggc cagcgcacgc attaacagtg cagaggaagc aatattgcgt
    12301 tgatggaact catcgcccat ggtaatgccc tgcgccatca ttgccgtgag atcgataccg
    12361 cgctccatgc gccccagcgc cgcgcttaat actggcatta acacatcgcg catccagcga
    12421 tggcgagtca ggacatcttc gccgtaagcg ccaaaacgca tcactttgcc gataccttcg
    12481 ttgaggttgc agtacgcccg gttgccgtcg gtcacgttct cgaccaccag catcggcata
    12541 ctggcagaag taatgccacc cattggcccg acggtattca catggtgaca aggaatgaag
    12601 ttcacttccc cctgctccag tattgccagc gcctgcgctt catctttcgc ccagccttcg
    12661 aacagacatg cgcccacgca cgcccctttc atgggtccgg tcatctcctg ccagcgcatt
    12721 ggcggcccgg cgtgaagcag tgttttgccc ttgtttagtt cgctgataag tgaagaagcc
    12781 ggttgcacat ccagccagtg tgaacgagcg cgacgaattt gttcgataac cgcagcattg
    12841 gcttgcgcca ctgatgtaaa catgggaacc ccttattgca aacgttctaa taaacgagcc
    12901 agttttttat tgccgccggc gactggcgac cattggtaat gcacaaccgg tttgctggcg
    12961 ctttgtagct ccagcgcaaa gctgcgtaat ccgatgttaa tcacggcgac gttttccagt
    13021 aatgacggtg tgtgttgctg tgtggcaggc gagagcggat gaattaacgc cgcagccagc
    13081 aaggtggctt ccggtagcga actcactacc gcgatccccg catcttccag cgtggcgatt
    13141 tgctgcgagc ggcattgcgg gtcacgttca gtgcctgtca ccgtggcaat ggcatacagt
    13201 ggttgattgt ctgaacgcgc ggcacaggct ttttgccagg cgctcaccag cgaggcggca
    13261 ggatcggcgg tcgcaccgaa gccaatcacg acatcaagca gcaacacgcg cacttgcggt
    13321 ttagcgccga gatcggcaat taacaggttg cgtaaggtcg ggtcgatcat cggatgggga
    13381 cgcccgacgg tgtaaaaatc atcgcccagg tcgaggatct gatgtccgtc ggcgtccagc
    13441 atcatgccat gttgatgggt atcgtcggct tccacgccaa ggtgtccggc aagtaatccc
    13501 gccgcttcag cagccagcgt accgccggta tacaaaccgc aaataaatcc gctgctgaca
    13561 ggcgctatcg cgttacgtcg cgcagtgacg cgtgaaagca gacaagccag acgagcggcc
    13621 tcatccagcg aggaggcaaa ccagacattc tcgtcgcggg ccaccgccgg ggtataacct
    13681 aaaaacagcg ccaccgtcgg tttgccagtt gctttcatgg cattaacaat tttcagacgc
    13741 acagtttcgg caggtggttt tgaaacaaat gccagcactt cgcttttctc gtctgcactg
    13801 agcatttcca gcgccgttag cgcactgatg ccgcccactt cacggctgag atcgcgcccg
    13861 ccaagaccaa tcgcgtgagt aattccctcc cctgccagcg caatctgtga gcacagctcc
    13921 tgaatcccgg taccggaagc gcctatgacg ccaatgttgc cttccggcat cacgttagca
    13981 aaagccagcg gcgtggcggc aatcatcgac gtaccgcagt ctggccccat caccagcaag
    14041 cctttttccc gcgcgcgggt tttaagttgg atttcatctt ccagcgtgac gttatcggag
    14101 aacatcatca cgttgaggtt gcgatccagc gcctggttcg ccagctccgc cgcatactca
    14161 ccagccactg aaatcagcgc cagattggca tcgggtaatt tctgactggc actgtcccag
    14221 cgacgcacct gcgtcaacgc ctggctgctg cctgaaccct gcgccagttg ttttagcgcc
    14281 tcttcaagct gctgcataac cgcctgcgcg atccccgcat ccgccgcttc gctacgaatt
    14341 gccacgcaaa tatcgttcgg cgtggcgtta ttaaaatcgt catgccagaa acctgtagta
    14401 tctaataacg ctttattggc gggcgtaccc atcattacgg aaacatcatc aacattttct
    14461 gattcgctga gttttcgtga aataatcatt aaactgaccg aatcctgaaa acaccctttt
    14521 ttaataaagg cgtggatcat aacaactcct taaaaattgc acccatggca attgcacgag
    14581 cagtgttaat gtggcgatta cggtatcgat gacaacgagt aaataaggtg atgaatatca
    14641 cacgtggatt attaacaaaa gaattcaaat aaccgccaga ctattacgcg ttatttttta
    14701 ataagcatgg ttatttgaaa aaacaggtta aaagcacgaa gattatcaaa taaattctaa
    14761 ctatattttt ttgcctgtct ggatcacata atccagatat tttccctgta tgttaattgc
    14821 agtcatgctt cacaccatcg ttaaaaagga agacagatga aaatcagtcg ggaaacactc
    14881 caccagctaa tcgagaataa actctgccag gctgggttaa aacgtgagca cgctgcaacc
    14941 gtggctgaag tattggttta cgccgatgcc agagggatcc actctcatgg cgcggtgcgc
    15001 gttgaatact acgcagaacg catttcaaaa ggcggcacca accgcgaacc ggaatttcgt
    15061 cttgaggaaa ccggaccgtg ctcggcaatt ttacatgccg acaatgccgc cggacaggtc
    15121 gcggcgaaaa tgggtatgga acatgccatc aaaaccgccc agcaaaaagg cgttgcggtg
    15181 gtcggtatca gccggatggg tcacagcggc gcaatctctt attttgtgca gcaggctgcc
    15241 cgcgccggat tgattggcat ttcgctgtgc cagtccgatc caatggtggt gccgtttggc
    15301 ggcgcggaaa tttactacgg tactaaccca ctggcctttg ccgcgccggg agaaggcgac
    15361 gaaatcctta cctttgatat ggcgactacc gtacaggcat ggggaaaagt gctcgacgcc
    15421 cgctcgcgca atatgtctat cccggatacc tgggcagtcg ataaaaacgg tgcaccaaca
    15481 accgatccat ttgcagttaa cgccctgctc cctgccgccg gaccgaaagg gtatggcctg
    15541 atgatgatga ttgacgtcct ctcaggcgtc ttactcggct taccgttcgg gcgacaggtt
    15601 agttcgatgt atgacgattt acacgccggg cgtaatttgg ggcaattaca tatcgttatt
    15661 aacccgaact ttttctcctc cagcgaatta ttccgtcaac atcttagcca gaccatgcgc
    15721 gaattaaata ccattacccc cgcgcccggt tttaatcagg tttattatcc cgggcaagat
    15781 caggatatta aacaacgcaa agccgccgtc gaaggcatcg aaattgttga tgatatttac
    15841 cagtatttaa tttccgacgc gctttataac acgtcatacg aaacgaaaaa tccctttgcg
    15901 caataattat tgagacagga ctttcttatg attacacatt ttcgtcaagc catagaagaa
    15961 acgctgccct ggctttcctc ttttggcgct gacccaacgg gtgggatgac ccgtttactt
    16021 tattcgccgg aatggctgga aacccagcag caatttaaaa aaagaatggc agcaagcggg
    16081 ctggaaacac gtttcgatga agtggggaat ttatacggtc gcctgagtgg caccgaatat
    16141 ccacaggaag tggttctgag cggttcgcat atcgataccg tggttaacgg cggtaacctc
    16201 gacgggcaat tcggcgcgct ggcggcctgg ctggcaattg actggctgaa aacgcaatac
    16261 ggcgcgccgt tacgtacggt cgaagtggtg gcgatggcag aagaagaagg cagccgcttc
    16321 ccgtatgtct tctggggcag taaaaatatc tttgggctgg cgaatcctga cgacgtgcgg
    16381 aatatctgtg atgccaaagg aaatagtttt gtcgatgcga tgaaggcttg cggatttact
    16441 ctgccgaacg ccccactaac tccgcgtcag gatattaaag cctttgtcga actgcatatc
    16501 gaacagggct gtgtgctgga aagtaatggg cagtcaattg gcgtggtgaa tgcaattgtc
    16561 gggcaacgtc gctatacggt gacgctgaac ggtgaatcaa accatgcagg caccacgccg
    16621 atgggttatc gtcgtgatac ggtttacgct ttcagtcgca tttgccatca gtcagtcgaa
    16681 aaagcgaaaa agatgggcga cccgctggtt ctgacctttg gcaaagtaga gccgcgcccg
    16741 aatacggtga atgtagtgcc gggtaaaacc acgttcacca ttgattgtcg tcataccgat
    16801 gctaccgtgc tgcgcgattt cacccaacag ttagaaaacg acatgcgggc gatttgcgat
    16861 gaaatggata ttggtattga tatcgattta tggatggacg aagaacccgt gccgatgaat
    16921 aaggagctgg tcgccaccct gacagaattg tgtgaaagcg aaaaactgaa ttaccgggtg
    16981 atgcacagtg gtgccgggca cgacgcgcaa attttcgcgc ctcgcgtgcc gacctgcatg
    17041 attttcattc ccagcatcaa cgggatcagc cataacccgg cggaacgcac caatattacc
    17101 gaccttgccg aaggggtcaa aacgttggca ctcatgcttt atcaacttgc ctggcagaaa
    17161 taaggagtta taaatgggat atttaaataa cgtcaccggt taccgcgatg atttactggc
    17221 taaccgtgcg attgttaaac acggtaattt cgcactgtta acaccagacg gtctggtaaa
    17281 aaatattatt ccgggctttg aaaattgtga cgcgacaatc ctctccacgc caaagctggg
    17341 tgcctctttt gttgattatc tggtcacact gcatcaaaac ggcggcaacc aacagggctt
    17401 cggtggcgaa ggcattgaaa cgttcctgta tgtgatctct ggaaatatca ctgccaaagc
    17461 cgaagggaaa acatttacct taagtgaagg tggctatctt tattgcccgc caggctcctt
    17521 aatgacgttt gttaacgtcc aggccgaaga cagccaaatc tttttatata agcgtcgcta
    17581 tattccggtg gaaggtcatg cgccgtggct ggtttctggc aatgccagcg aactggaacg
    17641 cattcattat gaaggcatgg acgatgttat tctgctggat tttctgccca aagagttagg
    17701 ttttgatatg aacatgcata tcctctcttt tgcaccaggt gccagccacg gttatatcga
    17761 aacccacgtt caggaacacg gtgcctatat tctttccggt cagggggttt ataacctcga
    17821 caataactgg atcccggtga aaaaaggcga ttacatcttt atgggcgctt attctttaca
    17881 ggctggttat ggcgtagggc gtggtgaagc gttcagctat atttactcga aagattgcaa
    17941 ccgcgacgta gagatttaag tcataccact cgcctgatta tttttagcta tgttggattt
    18001 tgccacggtt tattccgtgg ctttttttgt agtcaattca ggtaaaaaaa gatgcgatat
    18061 aaatcgcatc cgctttaaag gtaatagtca gtttttaatt ccctgaccta ttttaatggc
    18121 gcaggcaata tttcgcgcac tgttgaagag attggtttca ccgctggcga gcacttcggc
    18181 taaaggagcc agacgtggca aaatgctgaa taccgcatca atgccgtact ggtgcaccac
    18241 ttccacgcca tcacccaata cgccagcaat cccaatcacc gggacattaa actgcttcgc
    18301 caccgacgcc acacccagcg gcgctttacc gcctgccgtt tgcgagtcga tgcgcccttc
    18361 cccggtgatc accagtgctg caccctgcac cgcctgcgca agattgaccg cattcaacac
    18421 aatctcaata cccggtttaa tatccgcatt gaggaatacc gccgccgcaa tgcccatacc
    18481 gcccgccgcg ccgccacccg ccatctggca gacgttgatt tcagttagct gttgtaaaac
    18541 acgggcgtaa ttttgcagcc cctgttccag ctcttcgacc atctccggcg ttgccccttt
    18601 ttgcgggcca aacaccgccg ccgcgccgcg tgcccctacc agcgggttat caacgtcaca
    18661 tgctacttca atatgacagt tcgccagacg cggatcgcat tcattcatct caatacttgc
    18721 cacgcgcgct aaattgccgc cgttagccgc cagcggctga ccgtcggcat caaggaaacg
    18781 cacaccgagc gcctgcgcca tgcccatacc gccgtcgacc gtcgcactgc cgccaatgcc
    18841 gagaataata tggcgaatgc cattatccag cgcatggaga attaactccc ccgtaccaaa
    18901 actggaggcc agcaacggat tacgcttttc aggtgcgacc agcatcaggc cacttgctgc
    18961 tgccatctca atcaccgccg ttttcccgtc gccggtaatg ccataaaaag cattcacttt
    19021 ttcgcccatc ggcccgcaga cttcaagctt cacgatgtta ccgcccgtcg cggcgaccat
    19081 cgcctccacc gtcccttcgc cgccatccgc tatcggcaaa cagatatagt gcgcatcggg
    19141 aaatatggtc gaaaacccgg ctttaattgc ctgacaacat ttttctgcac ttaagctctc
    19201 tttaaaagag tctggcgcaa tgacaatctt cattatcaat cctttttcaa caaattattc
    19261 gatgcgttcc cacggaataa gattatccat aagaacagcc agtaaaattc cgaccagtaa
    19321 gccgttactg agcagaggac gaagcgttaa tggaaggtct tgcagataca caggcggtaa
    19381 tgccattaaa aatatgccga caaataacgg caatgcgagt cgataaatat tgcgagcggt
    19441 aaacgttatt tgctggctaa acaccagcgc ggaaaagagt aaaggcagat aagaaaccag
    19501 catgaccgcg ctactcacgg gtaaagggat actgcaaaac agtcgcgtga gtgccggaac
    19561 cagtgccacc agcaggcaaa taacgctgcc ataaataaac gaacgccgcg tgtaatcgcc
    19621 agtctgggtt aataaaccaa tggatgaaac aaacggtgaa aatggaatta ctgccagcgg
    19681 cacggttatc agtgtcataa atccggtcgc cacaaagctg cgacgataac gcgtattccc
    19741 cgcgccctgc tgcggataaa aaacatccgt gccccgaatc gcaccgtagg tattgctgat
    19801 atttaccaga cctgtaatca ccgccgtcag aataatcccc ggcgacaaag caccgccact
    19861 gccgagcggg aaccactgcc agtgcaactc accggagagc gaatgcgaag aaggaaagca
    19921 aaagtaccac aacaaccagc cggttattgt accgaccagc aggccgtaac gggcaaaacg
    19981 ttgtggcagg aaaataatca tcgccagtac caggcacatc accgccaccg agagcgcgaa
    20041 cggcggtaat tgaattttaa aattcgggtc ggctatgcca aacggcagcc cgagcatacc
    20101 tttgaaaaag atagtggtca gttgcgcacc cagcatcaac ataaacaaga ccatcacaga
    20161 cggcgtaaat aaccgtgcca ggcgatggcc taatccgcta aaaccaatca acatcgtcag
    20221 cacgccggag agcgcaatcc ccaccgccag actggtagcg atatcgttga tcggtgtccc
    20281 gcgcgatgct tcgccaaggg tgatagtcag gatggttccc caccacaggc cgcccggccc
    20341 ctccataata gcgcgacgat gaccgcagaa cgcctgagcg aagcaggcca gtgcggtagc
    20401 aagaaaagca tattgcgtga gcgtaagcag gctactttgc ggcaactgaa aagcagaaag
    20461 tagcgtagga ggaaccacaa ccgtgttgca aaaaataaag aaaaaccact gaaatcctga
    20521 taataggctt tcgcggccca ctgcaaaatt gaacataaac acaccttaac cggagaatgc
    20581 ccgccacaag gacgggcatt gcagggccag attactgctg atgtttaagg ataaattgac
    20641 ctttcggcgc aacagggaag ccctgttcga tatcgtaaat cacatcacca cgtaagatgg
    20701 ttttcgtgat acgcgcgcca atagtacggc caacatacgg gctgactttg tggcgatatt
    20761 ccagatcgtc attggtaaga acatagctgc tattcggctg aatgaagacg aagtcggcat
    20821 cttttcctgg ggcgatacgg cctttttgct gcagaccgaa aatatctgct gcgttagtcg
    20881 ccattaattt gccgaacatt ggcagagaca ttccgcgttt ctgtaccgct tcatcgaaca
    20941 tcacgtccat gcagctttgc agaccagcga taccgcccca cgctttcatg atgttaccgg
    21001 ctttcatttc cggcgggcat ggagagtggt cggaaaccag gcagtctatt tcaccgttaa
    21061 acagtttttc ccacatgcct ttctggtttt ccagatcgcg gatcggcggt gaacacttcg
    21121 ccagagtacc gatttcttcg aactgatcgg tatccagcac aaagtaatgc gggcaggatt
    21181 cacaagtaac atcctgacct tcctgacgtg cacgagtcac ttcctcaaca ccttccgggc
    21241 tgctgacgtg gcaaacgtgc agacggcaac cagcgacttt cgccaggtac agtacgcggc
    21301 gaatcgcttc cacttcggta aataccggac gcgaagccac atagtcatgg gcagttacgc
    21361 gaccttcacg cttcgcttct tcgcccagtg cgtcacagat cagcgcgttt tcgcagtgca
    21421 ccagcaccgg ctggcctagt tcacccagct tctgcgcgcc tttgaagaat tgccagtcat
    21481 tgacgtcacg gaagtcgttg tcgataccgc gatcgccaca ggtcgcaacg aagcatttga
    21541 aaccgacaac acccacttca tccaactcat gcagacggtc aatgttgtaa gacaccaggc
    21601 caccgagttg tgccgcatcg atagtcagct ttcctttagc ggcatcgaac ttcagttcaa
    21661 ttgaggcgcg gtcaaccgtt gcaggcagct ggttgagcgg catttcgatc atggtggtga
    21721 taccaccttt tgctgctgcg cgagtaccgg tttcataacc ttcccagtgg ctgcgacccg
    21781 gttcagaaat atgggtgtgc gcatcaacca tgcccggcga aaccaccaga ccagacgcat
    21841 ccataacgtc ttttgcatcg cccagatcct ggccgatagc agcaattttt ccgtctttaa
    21901 cggcgatatc tacaacgcga gcttcgtttt ctaaaataac ggtgccgttt ttaatgatta
    21961 aatcaaaaga cataacaaac tccttgtaaa atacagattg gtttttaact caatgttcaa
    22021 tatcgtgatt aattaaccta tgtttttttg ttctcctgtt ttttctgctg ttgtacgttt
    22081 ctttaataag gcgtaggccg caaaggcgac gatgacgccg acaaaccatg aaacacgtga
    22141 taacggttcc ataaagggaa taaacttacc gccaagagat aaaataacgg ccaccagagt
    22201 tactgaaaac gcggtgaggt taaaaccgtt atcgtaatat ttataatcgc caggtgcggt
    22261 gtacagttca tcaagattaa tttgcccacg catcaccaca aaataatgcg ccatcatcac
    22321 accaattacc ggaccaagca ttccgccgat aatatcgagg aacagataaa tactgtcctg
    22381 attttccatt aatttccacg ggcagatcag caggctgata atactggcaa tcagtacgcc
    22441 gtttttatag gtcagtttcg tcggtgcaat ggcggcaatc tgataaccag ccggaataat
    22501 attaccggtg gcgttagtag agatagtcgt catcagaata accagtaccg caaagaacga
    22561 ggcgaacagg ctgtcccaac gctgaacaat atccagcacg ttccaggtgt cagcgccgta
    22621 atgaatactg gctccggcaa taatacatac cccggcgacc gcaaacagaa tataggccac
    22681 aactaaaccc agcgtttgcc ccagcgcctg ctcacgaaac gagtgggcgt tttgcgtaaa
    22741 gtcggatgcg ctcaccgccg gtgccgccca gaccgcaacc accgcgttta tcaccaccag
    22801 aaacaggaag ccactgtttt ctgctttctg aataccgccc ggaatgtagt caaagattgg
    22861 accgatcccg accagcgaaa tcgcccaaat cgccataccg ccgaaaacga tatagatgca
    22921 cgggttaaga atggcagtga atttatttaa aacttttccg ccgccaaaac caataccaac
    22981 gttaaccagc cagaagagta aaaaagtaat taagcccggt agagaaaggc ctaacagcgt
    23041 gaaatcgcca ccgagagtca aaaatcccgg ccagattttg ccaatcagaa tcaagcaggc
    23101 cagtgacccc gcgtaacatt gcaggccaaa ccacataatg gcagcaatac cgcctcttaa
    23161 taatccggga aacagtgcac cacgtacgcc gtaagaagca cgcaggatca tggcaaacgg
    23221 tacgccgtat ttactgcccg cagcaccgtt taataccatt accgcggcaa tgaaaaaggc
    23281 gctgaggata attgccagca taatactaaa ggtagacaag ccgagaataa aaaagccgcc
    23341 aaccatcaca taattgggaa cgttatgaac cgaacccatc cataaggtaa aatagttaaa
    23401 tgttttccag gtccgctgac tttgcgtttt cggcagtaga tcttcgctat agccgcgttg
    23461 ctggaataat tttctctgat gttccataaa tccctcttaa tttcacgtat cgaataacgt
    23521 tgttttaatc tagctatgag gaacaaataa aataaaaata ttatttttaa tactgaagca
    23581 acatctttgg ctgctttctt tttttaatat tgctggccga ttcattttct gatatcggcc
    23641 atttttattg cgggtatcag gccagtttat ggttagccat taattccagc gcctgcacta
    23701 acgcagagtg atccaactgg ctgccaccgt ttgccgcaca ggtattaaac aattcctggc
    23761 aggtcgcggt gtttggcagg ttcagcgcca gtgctttcgc actttgcagt gccaggttga
    23821 gatctttctg gtgcagcgca attttgaaac ctggattaaa ggtgcgttta atcatgcgct
    23881 caccgtgaac ttccagaata cgtgaggaag caaagccgcc catcagcgcc tggcgcacac
    23941 gtaccgggtc cgcaccggct tttgaagcaa acaacaaggc ttcagaaacc gcttcaatat
    24001 tgagcgccac gataatctga tttgccactt tgcaggtttg accgtcgccg ttaccgccca
    24061 cgagagtgat atttttaccg agcagttcaa acagcggttt tacacgttca aatactgctt
    24121 catcaccgcc aaccataatc gataacgtcc cttcacgcgc gccgatttca ccgccggaga
    24181 ctggcgcatc gagataatcg ccgcccagtt cattcacctg acgagcgaaa cgtttagttt
    24241 caatcgggga aatggagctc atatcaacaa tggttttgcc cttcagcgag gctttggtac
    24301 aaccattttc accaaacaga acgtcttcaa cctgtggcgt atccggcacc ataataaaaa
    24361 tgatgtccga tgcatctgtt acctggcgcg cagtttcaac actaacggcg cccagtgaca
    24421 gtaattcatc agcaaccgga ccaatggttg tgacatgcag ttgatgaccg gcacgcgcca
    24481 gattaatggc catcggtgta cccataatgc ctaagccaat aaatcccagt gtcataatta
    24541 acctctttta aatttcgctt ttcctgaatt cagacaacac gatcccctgc caacttagcg
    24601 gcctaaaaaa gcattgtctg aatagcgtta cgttttaacg gtacggatcc atccagcgta
    24661 aaccggcttc cgtggtggtt tggggtttat attcacagcc aacccaaccg ttgtagtcag
    24721 aattttcaat taccgtaaag agatattcgt aattaatttc tccggttccc ggttcgccgc
    24781 gatgcggatt atcagcaatt tgcaggtgac caattttatc ggcccactga gtcatggtat
    24841 tagttaattc accttccatc cgctgcatgt gataaatgtc atactgaatt ttcaaattgc
    24901 agcgaccaa
//
LOCUS       DAZFJL010000069        24671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFJL010000069 DAZFJL010000000
VERSION     DAZFJL010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.069"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(158..349)
                     /gene="yfgG"
                     /locus_tag="ACHJ8C_002669"
     CDS             complement(158..349)
                     /gene="yfgG"
                     /locus_tag="ACHJ8C_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO5552506.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            367..519
                     /locus_tag="ACHJ8C_002670"
     CDS             367..519
                     /locus_tag="ACHJ8C_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5552507.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMS"
     gene            701..2944
                     /gene="pdeF"
                     /locus_tag="ACHJ8C_002671"
     CDS             701..2944
                     /gene="pdeF"
                     /locus_tag="ACHJ8C_002671"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO5552508.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FKLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERISALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREVVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2983..4524)
                     /gene="ppx"
                     /locus_tag="ACHJ8C_002672"
     CDS             complement(2983..4524)
                     /gene="ppx"
                     /locus_tag="ACHJ8C_002672"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO5552509.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFHGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4529..6595)
                     /gene="ppk1"
                     /locus_tag="ACHJ8C_002673"
     CDS             complement(4529..6595)
                     /gene="ppk1"
                     /locus_tag="ACHJ8C_002673"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO5552510.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQSE"
     gene            complement(6766..7404)
                     /gene="purN"
                     /locus_tag="ACHJ8C_002674"
     CDS             complement(6766..7404)
                     /gene="purN"
                     /locus_tag="ACHJ8C_002674"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO5552511.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLITSAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7404..8441)
                     /gene="purM"
                     /locus_tag="ACHJ8C_002675"
     CDS             complement(7404..8441)
                     /gene="purM"
                     /locus_tag="ACHJ8C_002675"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO5552512.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8766..9392
                     /gene="upp"
                     /locus_tag="ACHJ8C_002676"
     CDS             8766..9392
                     /gene="upp"
                     /locus_tag="ACHJ8C_002676"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO5552513.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            9478..10767
                     /gene="uraA"
                     /locus_tag="ACHJ8C_002677"
     CDS             9478..10767
                     /gene="uraA"
                     /locus_tag="ACHJ8C_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO5552514.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISMLRPEEVVLDAEDADITDK"
     gene            10862..11563
                     /gene="hda"
                     /locus_tag="ACHJ8C_002678"
     CDS             10862..11563
                     /gene="hda"
                     /locus_tag="ACHJ8C_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO5552515.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11701..12060)
                     /gene="arsC"
                     /locus_tag="ACHJ8C_002679"
     CDS             complement(11701..12060)
                     /gene="arsC"
                     /locus_tag="ACHJ8C_002679"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO5552516.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEVLIQAMVENPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(12081..13544)
                     /gene="bepA"
                     /locus_tag="ACHJ8C_002680"
     CDS             complement(12081..13544)
                     /gene="bepA"
                     /locus_tag="ACHJ8C_002680"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO5552517.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPIVVQSSEDFYL
                     AKVRTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANDAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13757..14818
                     /gene="yfgO"
                     /locus_tag="ACHJ8C_002681"
     CDS             13757..14818
                     /gene="yfgO"
                     /locus_tag="ACHJ8C_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO5552518.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRPWATSIVLILFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQD"
     gene            complement(14917..15387)
                     /gene="bcp"
                     /locus_tag="ACHJ8C_002682"
     CDS             complement(14917..15387)
                     /gene="bcp"
                     /locus_tag="ACHJ8C_002682"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO5552519.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(15387..15959)
                     /gene="gcvR"
                     /locus_tag="ACHJ8C_002683"
     CDS             complement(15387..15959)
                     /gene="gcvR"
                     /locus_tag="ACHJ8C_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO5552520.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            16105..16983
                     /gene="dapA"
                     /locus_tag="ACHJ8C_002684"
     CDS             16105..16983
                     /gene="dapA"
                     /locus_tag="ACHJ8C_002684"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO5552521.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            17000..18034
                     /gene="bamC"
                     /locus_tag="ACHJ8C_002685"
     CDS             17000..18034
                     /gene="bamC"
                     /locus_tag="ACHJ8C_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO5552522.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSEWHELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            18247..18960
                     /gene="purC"
                     /locus_tag="ACHJ8C_002686"
     CDS             18247..18960
                     /gene="purC"
                     /locus_tag="ACHJ8C_002686"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO5552523.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            19128..19991
                     /gene="ypfJ"
                     /locus_tag="ACHJ8C_002687"
     CDS             19128..19991
                     /gene="ypfJ"
                     /locus_tag="ACHJ8C_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO5552524.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            20006..22021
                     /gene="tmcA"
                     /locus_tag="ACHJ8C_002688"
     CDS             20006..22021
                     /gene="tmcA"
                     /locus_tag="ACHJ8C_002688"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO5552525.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEERWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWDEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTANNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLYQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHSFELQRPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGYRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLMRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALFALDDVRTERLRDRITQWQFFH"
     gene            22095..22793
                     /gene="ypfH"
                     /locus_tag="ACHJ8C_002689"
     CDS             22095..22793
                     /gene="ypfH"
                     /locus_tag="ACHJ8C_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5552526.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(22891..23091)
                     /gene="ypfN"
                     /locus_tag="ACHJ8C_002690"
     CDS             complement(22891..23091)
                     /gene="ypfN"
                     /locus_tag="ACHJ8C_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO5552527.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(23119..24246)
                     /gene="dapE"
                     /locus_tag="ACHJ8C_002691"
     CDS             complement(23119..24246)
                     /gene="dapE"
                     /locus_tag="ACHJ8C_002691"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO5552528.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFINELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(24250..24606)
                     /gene="yffB"
                     /locus_tag="ACHJ8C_002692"
     CDS             complement(24250..24606)
                     /gene="yffB"
                     /locus_tag="ACHJ8C_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO5552529.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAATLMTEMPAIIKRPLLCAPGKPM
                     LLGFSESSYQQFFHEV"
BASE COUNT         5793 a         6108 c         6473 g         6297 t
ORIGIN      
        1 gatgtattta tttcgcagcc gaacaggatg aacttcagtc agaacaaaga cgtaaaattt
       61 aagatgaaag aatatctgcc gctttgataa tctcaaagag caaaagcatc cgacagcgag
      121 gtgccgttat gttctgtgag agtggagaag gtaaccgcta acgttgtacc ggtgattcga
      181 cggagagtgt agattgctga gcttcttttt tgctctgatg gtgctgccag gcaccgacgg
      241 acgagtagat aaaacggcca aagaagaaga taaagctgat gagcagtacg atacgggtca
      301 tgcgactgtt aaatcggtgt cgttttcgca tactggttgc ctgactcaca aaaggttcct
      361 tgaagtatgt cccacgctgt ggacggtact tacattaagg cacaacagga caaaatggtc
      421 aattcttcgt tatgtaaaaa agcgtcagtg gatacatatt ttaatgttat ggaagttaat
      481 ttaattattt acaatgatga tgtaataatg atgagttaac atgataaacg taataattca
      541 ttaatcaggg ttattttgtt tgatatatat caattggaac tttcatatga cacttagaat
      601 caatgctctc tatagtgatg aataatcatc attcgaagtc aggtgggatg cctgtctgaa
      661 tacacctcct tcaggatgtg ggggattctg ctgagcatct atgaaactga atgcaactta
      721 tataaaaata cgtgataaat ggtgggggct tccgctgttc ctgccttctt taatcttgcc
      781 cattttcgcc cacattaata ctttcgcgca tatttcttcc ggtgaagttt ttctctttta
      841 tctgccactg gcactgatga tcagcatgat gatgtttttc agctgggcgg cattgccagg
      901 gatcgcctta gggatttttg tccgcaaata tgcagagctg ggtttttacg aaacgctctc
      961 attaacggct aattttatta tcattatcat tctctgttgg ggcggttaca gggtctttac
     1021 tccccggcgt aacaacgttt cacatggtga tacccgttta atttcccagc gtatattctg
     1081 gcagattgtg tttcctgcaa cgctgtttct gatacttttc cagtttgccg catttgtagg
     1141 attactggcg agtagagaaa atctggtcgg cgtcatgcct tttaaactcg ggacattaat
     1201 caattatcag gccttactgg tgggtaatct gatcggtgtc ccgctgtgct acttcatcat
     1261 tcgtgtagtg cgaaatccgt tttatttacg tagttattat tcgcaattaa aacagcaggt
     1321 tgatgtcaaa gtcaccaaaa aagagttcgc actctggtta ctggcattag gtgctttact
     1381 gctgctgtta tgcatgccgt taaatgaaaa aagcacaatt tttagcacca actatacctt
     1441 gtcattattg ctgcccctga tgatgtgggg agcgatgcgc tatggttata agctgatttc
     1501 gctgctctgg gcggtcgtgt tgatgatcag catccacagc tatcaaaatt acattcccat
     1561 ttatcctggc tataccacgc agctgaccat aacctcctcc agttatctgg tattctcttt
     1621 tattgtcaat tatatggctg tactggcaac ccgtcagcga gcggtagtca gacgcattca
     1681 gcggcttgcg tatgtggacc cggtggttca tctgccaaat gttcgcgccc tgaatcgcgc
     1741 gttgcgtgat gccccctggt ctgcgctttg ttatttacgc atccctggca tggaaatgct
     1801 ggttaagaac tatggcatca tgctacggat tcaatacaag caaaaacttt ctcactggct
     1861 gtcacccttg ctggaaccgg gtgaggatgt ttatcagctt tcgggtaacg atctcgcgct
     1921 gcgactgaat acagaatcgc accaggagcg cattagcgca ctggatagcc atctcaagca
     1981 atttcgtttc ttttgggatg gaatgccgat gcaaccgcag attggcgtca gttactgcta
     2041 tgtgcgctcg ccagtgaatc atatctacct gctgctggga gagctaaata cggtcgccga
     2101 gctttccatc gtgaccaacg ccccggaaaa tatgcagcgt cgcggggcaa tgtatttgca
     2161 acgcgaattg aaagataaag tcgcgatgat gaatcggcta cagcaggcgc tggaacacaa
     2221 ccattttttc ctgatggccc agccgattac cggtatgcgt ggtgatgtct accatgaaat
     2281 tcttctgcgc atgaaaggtg agaatgatga actgatcagc cccgacagct tcttgccggt
     2341 cgcgcacgaa tttggtttat cgtcgagtat cgacatgtgg gtcattgagc atacgctgca
     2401 atttatggct gaaaacagag cgaagatgcc cgctcaccgt tttgcgatta atctgtcgcc
     2461 aacctcggta tgtcaggctc gttttcccgt tgaagtcagt cagctgctgg ctaaatatca
     2521 aattgaagcg tggcaactta tttttgaagt caccgaaagt aatgctctga ccaatgttaa
     2581 gcaggcgcaa atcaccttgc agcatcttca ggaattaggc tgccagattg cgattgatga
     2641 tttcggcacc ggctacgcca gctatgcgcg gcttaaaaat gtgaatgccg atctgcttaa
     2701 aattgacggc agttttatcc gcaatattgt gtcaaatagt ctggattatc agatagtggc
     2761 gtcgatttgc cacctggcgc gaatgaagaa aatgcgggta gtggcagagt acgttgaaaa
     2821 cgaagagatc cgcgaggtgg tgctctcttt ggggatcgat tatatgcagg gttatcttat
     2881 tggtaagccg caaccgttaa ttgatacgct gaatgaaatc gaacccattc gcgaaagtgc
     2941 ctgaataatg cgggccgaca tttctcgtcg gcccgcaaag cattaagcgg cgatttctgg
     3001 tgtactttct tcttcaattt tcaaccgcca gccagccacg ccttcccagt attcttgctc
     3061 cttttccaga tcaagcagta ccagcgcatt ctgactaaac cagtcatgcg gaaaacgcag
     3121 tgtccagtga ctgtcatcgg taatcagcgt caatgttggc ggtgtggtgg ttgcctgacg
     3181 ttgattattg agtaatacgc caaggcgcaa tagctgaatc agtggcagga actgtttctt
     3241 cttaaacaag gtaaagcgcg gcagatcgtc gagcttaatc gctttacggt gatagcgcac
     3301 cagcgtcgcc atcatcaact gctgttcctg attaaaaccc ggcaagtcgc tgttttgcag
     3361 aatataagcg gagtggcgat gcaaaccgct gtggttgata ttcaacccga cctcatgcag
     3421 catggcggcc catcgcagta gcgcctccag ttgcggatgc gccagcttcg gttgctgttc
     3481 ccgccactgt tcgtacattt gcatagtggt atccagcacc cgtcgggcct gttcactgtc
     3541 gatgtgatac tggttggcga ggctgctggc agtgcgacta cgcacatcct gatgacggaa
     3601 acgtccttcc atttcataca gtacgccttc gcgtaacgcc ccgtcagaaa ggcgcagttc
     3661 acggatggct aaagcatcaa acacaccgca taaaatcgcc agtccaggaa cgaagactgt
     3721 tttccgctct tcggaaagac ctggtaaact cagcgatgcg aaattacggt gccgtaaaac
     3781 ttcttttacc agtttttcca gacgttccgg ggtaattatc ccgtctttct cgcccatttc
     3841 cattaacact tcatgggcgg cttttatggt accggaagcg cccattgcca cgttccagcc
     3901 ctgaattcga aattgccagg ttaacgtttc cagtttttgt gctgccgcca tgcgagcgcg
     3961 ctgaaaattc tctttattga tgaccccgcc atgaaaataa agctgggcaa agctgacaca
     4021 acccatccgg cggctttcaa cgagaatcgg ttcgaaattt tcaccaatta ccagttctgt
     4081 agaaccgccg ccaatatcaa taaccagttt gcgacctttt tccggttggg tatgttccac
     4141 gcccataaaa atcagacggg cttcttcatt accggaaata atttcgatcg ggtagggaat
     4201 gactttttcc gcgcgtttca gaaagtcagt ggcgttcagc gcctgacgca gcgtatgggt
     4261 accaactata cagacgctgg caggagaaaa cccctgtagc ctttcggcaa atagcgacag
     4321 acagttcaaa ccgcgcgtca ttgcctcttc actcaacata ttatctggcc ccagcccgtc
     4381 cgccagatgc acccgctgtt tcaggcggcc aataatctgc atggcaccat ctaccacacg
     4441 ggctatgacc atgtgaaaac tgtttgaacc aagatcgacc gcagcaaact cctgcggacg
     4501 aggggattta tcgtgtattg gcatagggtt attcagattg ttcgagtgat ttgatgtagt
     4561 cgtaaatcgc caactgcgcc cgtactttgc ggcgattgcc gcggggaacg tagcgattac
     4621 tgagttcttt atcgatataa cgtgctttga ccgtatcgct gaacaatatg tcgatgatgt
     4681 ccagcacccg ctgcttcagg cgcggatcga gcagcggcgt cgccacttca atacgataat
     4741 caatattgcg cgtcatccag tcggcggaag aaaggtagac ctttttatcg ccgccatttt
     4801 caaaaatata aacccggtca tgttcaaggt aacggtcaac aatactgatg gcacgaatgt
     4861 tgtcgctaat gccttccaga ttggggatca gcgaacacat gccgcgaacc agcagattaa
     4921 ccggtacgcc ggagctggag gccgcataca gacgatcaac caggccttta tcgacaaggt
     4981 tatttagctt cagggtgata ccgctgggca gcccttgctg cgcgttggcg atttcgcggt
     5041 ccaccatttc atacaacagg cggcgggagt tttgcggcga caccattaaa taatcaaatg
     5101 tcaccggacg gtatgggttt tcaataaagt tgaacacccg ccgtacttcg ttggtgatgc
     5161 gcgcatcggc ggtcagcaac gaatagtcag tataaagacg cgcggttttt tcgttaaaat
     5221 tcccggtccc gatgtgtgcg taacgcacca cttcaccgtt ttctttacgt gaaatcagga
     5281 acagtttggc gtgaattttc agccccggcg cagagaagat aacgtgcacg cctgcttcgg
     5341 tcaggcgctt cgcccagtga atgttggctt cttcgtcgaa acgcgcctgt aactcaacca
     5401 ccacggtgac tttcttaccg ttgtgtgcgg cgtggatcat cgagtcgatg atgcgtgaat
     5461 ctttcgccac gcggtaaatg ttaattttaa tcgccagtac gctcgggtcg aacgaagcct
     5521 gacgcagcag ttccagtaca tgctcaaagg tatgataagg ataatagagc aacacatcgc
     5581 gttcgcgaat agcatcaaaa ccattgcgga actgggcttt atcaaaccaa atgtggcgta
     5641 aacgcggcag tggtttgttc accagattgg ctttgccgac attggggaaa ttaataaagt
     5701 ctttaaaatt atgataacga ccaccgggga cgatggagtc gtagcgggaa atagtcagtt
     5761 tttcgcgtaa cacttcaacc agcgcattgg gcatatcgcg ctgataaaca aaacgcaccg
     5821 gctcagcagt taaacgctgc ttgagactgg aagacatcaa ctccatcagg ctggcttcca
     5881 tctcatgcac taaatcgtat tcggcatcgc gggtcatctt cattgaatag gcattcagcg
     5941 cgtcataatc aaagaagcct ttgaaaatat catcgaggca gtaacgcaga atgttatcca
     6001 gaagaatcat cggcttgcgt cgacgtggcg cttctggcgg taaattcaca aagcgcggca
     6061 ctttatctga tgggatctcc agcagcgcgt aacggatggt atcgccacgg ataatttcca
     6121 ccgccagata ggtgtaatca tctttcagga actgcactaa gtcagtgtca ggattgatta
     6181 aaatcggcgt aatgtgctga cgcagatact gcttaaaata atgacgcagc cagttttgtt
     6241 gattgacgga gagctggcgt tcattaatca ggaagatctg gttgcgcgcc atctccagca
     6301 acagctcgtt gtagaggccg tcgaattcct gatcggcttt cagcacccgg gactgaattt
     6361 tgcccagtaa atggcgggaa tgagagttgg aaccttgttc ttcgctaata atgatgcgtc
     6421 gcttcagttc agcgaagcgg actttataga actcatcaag gttattggaa tagatcccca
     6481 ggaaacgcat cctttcaatc agcgggttag atttgtccgc cgcttcctga agcacgcgtt
     6541 cattgaacga taaccaactg agctcttttt cgatgtatag cttttcctga cccattacca
     6601 cttttactcc gttttattca cgggacactg cctggatatt atggcgagca atattggcag
     6661 atgtccaact atgccagaaa agggggaaaa aaactgctgg agcttgcgat ttacaggtga
     6721 attgaaaaac gccagcggca gagctggcgt tttaactacg gggcattact cgtcggcagc
     6781 gtagccctgc ggcggcagac gttgaccatc cagccacgcg gcgttttcgt gcattttcag
     6841 acgaccatcg gcaaaccagc taatcaccag tggataaata gcgtgttcct gggtttgcac
     6901 gcgggcggtg atgtcatctt ccgtatcacc agcaaatacc gggactttcg cctgtaaaat
     6961 aaccgggcca ccgtccagtt catcggtgac gaaatgcacc gatgtaccgt gctcctcatc
     7021 gccattttcc agcgcctgac gatgggtgtg taatccggga tatttcggca gcagagaagg
     7081 gtgaatgttc agcaaacgcc cggcatagtg ggagacaaac gccgggctga gaatgcgcat
     7141 aaaaccagcc agcacgacca catcgggtgc gtacatgtcg atttcatgaa tcaactcccg
     7201 gtcataggct tcacgactgt caaacgcgct ggtgatgagc gtatgcgttg caatacccgc
     7261 ctggcgggcg cgttcaaggc cgaacgcgtc ggccttattg ctgaaaactg cccgtacggt
     7321 gcctttaatt ttgttggttt tgcaggcgtc aataattgcc tgcaaattac ttccgttgcc
     7381 ggaaataagc accacaatat tcattattcg ataaccacgc gttgttcgga atcagaggct
     7441 ttgatgatac cgattttcca cgcgttttca ccgttggcat tgagcagggc gagagctttg
     7501 tccacttccg gagcaggcag ggcaataatc ataccgacgc cgcagttgaa ggtgcgatac
     7561 atttcatggc gctcaacatt acctgccgtt tgcagccagt tgaacacttc cggccactgc
     7621 caggaagatt catcaatcac tgcctgggta ttatctggca atacgcgcgg aatgttttcc
     7681 cagaagccgc cgccggtcag gtgcgcaatg gcatgcacat cgaccttttc aatcaactcc
     7741 agcactgact tcacgtaaat gcgggtcggt gccagcagat gatcggctaa tggcttacca
     7801 tcaagttcgg tggtttgcgg atcacaaccg ctgacttcaa gaattttgcg caccagcgaa
     7861 tagccattcg agtgcgggcc gctggagccg agtgcaatca gcacatcgcc gtcgctgact
     7921 ttagatccgt cgataatttc tgatttttct accacgccaa cgcagaaacc agcgacatcg
     7981 taatcttcac cgtgatacat ccccggcatt tctgccgttt cgccacccac cagtgaacag
     8041 cccgattgca gacaaccttc cgcgatgccg ctgatcaccg ctgaagcggt atcaacatcc
     8101 agttttccgg ttgcgtaata gtcgaggaaa aacagcggtt ccgcaccttg caccaccagg
     8161 tcattaacgc acatggcgac cagatcaata ccaatggtgt cgtgacgttt taagtccatc
     8221 gccagacgca gcttggtacc tacgccgtca gtgccggaaa ccagcacagg ttcacgatat
     8281 ttttgcggca atgcacacag tgcaccgaag ccgcccagac cgcccatcac ttccggacga
     8341 cgcgttttct tcactacgcc tttgattctt ccaaccagag cattacccgc gtcaatatca
     8401 acaccggcat ctttgtagct aagagaggtt ttatcggtca ctgcttgggt ccccacgcgt
     8461 tacttgcggt agaaaaataa aattcggcgc aattctaaca gggaaagcaa acgtttgcga
     8521 gactgcttta cacaaccttt ttgcacgtct tttccccagg cgcgcggcga aagaatactt
     8581 gtaccagggc aaaggatcgt tttcggatgg aataatcttc tttcataacc atttgaatat
     8641 aaaataactt tatctcaaac cgttatcatt ttgactaaag tcaacgaaaa taatattgcc
     8701 gccttgaaga aaggaggtat aatccgtcga ttttttttgt ggctgcccct caaaggagaa
     8761 agagtatgaa gatcgtggaa gtcaaacacc cactcgtcaa acacaagctg ggactgatgc
     8821 gtgagcaaga tatcagcacc aagcgctttc gcgaactcgc ttccgaagtg ggtagcctgc
     8881 tgacttacga agcgaccgcc gacctcgaaa cggaaaaagt cactatcgaa ggctggaacg
     8941 gcccggtaga aatcgaccag atcaaaggta agaaaattac cgttgtgcca attctgcgtg
     9001 cgggtcttgg gatgatggat ggtgtgctgg aaaacgttcc gagtgcgcgc atcagtgtcg
     9061 tcggtatgta ccgtaatgaa gaaacgctgg agccggtacc gtacttccag aaactggttt
     9121 ctaacatcga tgagcgtatg gcgctgatcg ttgacccaat gctggcaacc ggtggttccg
     9181 ttatcgcgac catcgacctg ctgaaaaaag cgggctgcag cagcatcaaa gttctggtgc
     9241 tggtagctgc accagaaggt atcgctgcgc tggaaaaagc gcacccggac gtcgaactgt
     9301 atactgcatc gattgaccag ggactgaacg agcacggata cattattccg ggcctcggcg
     9361 atgccggtga caaaatcttt ggtacgaaat aaagaataaa aattattaaa gccgacttta
     9421 agagtcggct tttttttgag taaagcgcct ataacacata atacagagga taatactatg
     9481 actcgccgtg ctatcggggt gagtgaaaga ccgccacttt tacagacaat cccgcttagt
     9541 ttgcaacatt tgttcgccat gtttggtgca accgtcctgg tgcccgtctt atttcatatt
     9601 aacccggcga ctgtactgtt atttaacggt ataggaacac tgctgtatct cttcatctgt
     9661 aaagggaaaa ttccggctta tcttggttcc agctttgcct ttatttcacc ggtattgtta
     9721 ctgttgccgt tggggtatga agtcgcgctg ggcggcttta ttatgtgcgg cgtgctgttc
     9781 tgcctggttt cttttatcgt gaagaaagcg gggaccggct ggctggacgt gctgtttcca
     9841 cctgcggcaa tgggcgcaat cgttgccgtc atcggtctgg agctggcggg cgtagctgcc
     9901 ggtatggcag gtttactccc ggctgaaggg caaacgccag actccaaaac catcatcatc
     9961 tccattacca cgctggcggt tacggtttta ggttccgtgc tgtttcgtgg tttcctggca
    10021 attatcccga ttttaattgg cgtgctggtg ggctacgcgc tctctttcgc aatgggaatt
    10081 gtcgatacca cgccgattat taatgctcac tggtttgcgc tgccaaccct ctatacgccg
    10141 cgcttcgagt ggtttgccat tctgactatt ctgccagcgg cgttagtggt tattgccgaa
    10201 cacgtagggc acctggtcgt aacggctaat atcgtcaaaa aagatctgct gcgcgatcca
    10261 ggtctgcacc gttcgatgtt tgctaacggc ttgtcgaccg tgatctccgg tttctttggt
    10321 tccacaccaa acaccaccta tggggaaaac atcggtgtga tggcgatcac ccgtgtttac
    10381 agtacctggg ttatcggcgg tgcggcgatt ttcgctatcc tgctttcctg cgtcggtaaa
    10441 ctggctgctg ctatccagat gattccattg ccggtgatgg gcggcgtttc gctgctgctt
    10501 tacggtgtca tcggtgcttc cggtattcgt gtgttgatcg aatcgaaagt ggattacaac
    10561 aaagcacaga acctgatcct gacttccgtt attttgatca tcggcgtcag tggcgcgaag
    10621 gtaaacattg gcgcggcgga gctgaaaggt atggcgctgg cgaccatcgt cggtatcggc
    10681 ttaagtttga tcttcaaact gattagtatg ctgcgccctg aagaagtggt gctggacgca
    10741 gaagacgcgg atataacaga caaataatgt accgaccggg cagtgttgct gcccggttca
    10801 aacatcatgg gattctgtgg taaacttctc gcgattttgt gaaatcctgg ttgaggtatc
    10861 tctgaacaca ccggcacagc tctctttgcc actttatctt cctgacgacg aaacctttgc
    10921 aagtttctgg ccgggggata actcctcttt actggccgcg ctgcaaaacg tgctgcgtca
    10981 ggaacatagc ggttacatct atctctgggc acgcgaaggc gcggggcgca gccatctgct
    11041 gcacgcggct tgcgcggaat tgtcgcagcg tggcgatgcg gtgggctatg tcccgctgga
    11101 taaacgcacc tggtttgttc cggaagtgct cgacggtatg gagcatttgt cgctggtctg
    11161 tattgacaac attgagtgta ttgccggcga tgagttgtgg gagatggcga ttttcgatct
    11221 ttacaatcga attctggaat cgggcaaaac acggttgttg atcaccggcg atcgtccacc
    11281 gcggcagttg aatcttggat taccggatct cgcgtcgcgt ctcgactggg ggcagatcta
    11341 caaattgcag ccactttctg atgaagataa gttgcaggcg ctacagttac gcgcgcgttt
    11401 gcgtggtttt gaactgccgg aagatgtggg gcgtttcttg ctgaagcggc tcgacagaga
    11461 aatgcgcacg ttgtttatga cgctggatca gctggatcgt gcgtcgatta ccgcgcaacg
    11521 caagctgacc attccgtttg tgaaagaaat tctgaagttg tagataaggt gtttattgtc
    11581 ggatgcggcg cgaacgcctt atccgaccta cgcgaagtta atccatagta ttcgtaggcc
    11641 ggataagacg cggcaagcgt cgcatccggc aatgtgcttc aactagcgat ggagcacatc
    11701 ttaacccacg atttccagta cctgctccgg tggacgacca atccgcgctt tgccattcgc
    11761 gaccacaatc gggcgttcca ttagcttcgg attctctacc atcgcctgaa tcagcacctc
    11821 ttcgctaagc gaactgtcag ccaggttcag ttctttataa agatcctctt tttggcgcat
    11881 caattcacga gcgctattca tccccagcat tttcagcaaa tcgcgcagcg ttgccgcatc
    11941 ggcgggtgtc tcaaggtaga gcactacttc tggctccacg ccgttttctt tcagcaggtt
    12001 cagcgtttcc cggctctttg aacagcgtgg gttatggtag atttttacct gtttggtcat
    12061 tgttcttcct ttaatgcgaa ttacatcttg gtataaggct taaagcgttc ctgcagctgg
    12121 cgcaactggt cgatgcgcgc atcgtaacgc gcttgttgca ggctgcctaa tttcacctgc
    12181 gaactggcgc tactcaacaa ggaaatagcc tgatcgagtc gcccggcgag cgcataacct
    12241 tctgctcgcg cagccagttc ctgatcgcgg ttatttagtg cggcttccgc ctgtgccagt
    12301 aaatcccagc cgttgctgtc atctttattg ttaaaagtgt agcgattcag aatattggcc
    12361 gcttcctgtg gttgtccccc ttgcagataa gcgttcgcca ggttgagctg caacaccgga
    12421 ttggtgcgca aatcgcgggc atttttcagg cgattgatag cgtcattggc tttgttttgc
    12481 ccaagatcga tatcggtagc cagatcgaga taccatgcgt tgccaggttc tgccgccagt
    12541 aacggttgca gcgttttacg cgcctcgtcg tatttattgg cttccatcgc ctgtaaagca
    12601 cgaccatatt gcgccgctcg ttgctgacga acgtttcctt tcgcccattc atccagcaaa
    12661 tcactggtga gttggttacg ccctgaattg tacatcccca gtgtgcgcac ttttgccaga
    12721 tagaaatctt ctgacgattg caccactatc gggcgcatct gattggcacg gttacgggca
    12781 tcagccagac ggctttccgg tagtgggtga gtgagcagaa tttctggcgg gcgcgaagag
    12841 taacgcgcct gatcgagtaa tttttcgagg aaggtcggca tcgcctgcgg atcgaatccc
    12901 gagcgttgca gcacctgaat accaatgcgg tccgcttcct gttcattttg ctgggtgaaa
    12961 ctgatcatcc cctggcgcgt tcccgccagt gtaccggtca gcgccgccat ccccgcctgt
    13021 ggactggcca tcgccagtaa aatagaacct aacgcgccga cccaggtcag cggcgcgctg
    13081 cgctgctgat cttccatcgc tcgcgccagg tgacgttggg tgacgtggga gatttcgtgc
    13141 gccataactg aagccagttg actttcgtta tcggaataac ggaacagggc agagtgcagc
    13201 accacgttgc cgccaaagaa agcaaaggcg ttaatttcat cgttgttgat cagaaagaaa
    13261 tgaaacggtg tcttaaccga attggcatgc gaaaccagac gcattcctag cgaattaatg
    13321 tattgtgtta acagcggatc attaattaac ggcgcgctgc cgcgtaactg gcggacataa
    13381 tagtcgccca tctgcatttc ctgaccaatg gaaagcgtgc ttcctgcgga ggttcccata
    13441 tccggcaagg tgtctgcgct gtcggcaaac gccggggcta cctgaccaat ggtcatagca
    13501 gcaatgaggg ttgcaaccag gttttttttc aactgcctga acataacctc tatcctgtat
    13561 ttctggagat agtcatttga ccgatactgt gaaatatcgt tcccaccgcg ccgcctttga
    13621 gtgtaacggc gaagattagc ccactcaaat gacttctcag tttcctgaat ccgtcataaa
    13681 aagcgaagtc ttttttgtga catttcgata caattgcgga tcgcaatccc gctcttgagg
    13741 ttcagggaag gtctgtatgc tcgaaatgtt gatgcaatgg tatcgccgcc gttttagcga
    13801 cccggaagcg attgccttgc tggttatttt ggttgctggt tttggcatta tctttttctt
    13861 tagcggccta ctcgcgccgt tgctggtggc tattgtgctg gcctatttgc tggaatggcc
    13921 gactgtccgc ctgcagtcta ttggctgttc ccgcccttgg gcgacatcta tcgtgctgat
    13981 cctgtttgtg ggcatcctgc tactgatggc gttcgtggtt ctgcctattg cctggcagca
    14041 gggcatctac ttaatccgcg atatgccagg gatgcttaat aaactttctg attttgctgc
    14101 cacgttaccg cgccggtatc cggcattaat ggatgcgggg attattgatg caatggctga
    14161 aaatatgcgt agccggatgc tgaccatggg cgattcggtg gtgaaaattt ccctcgcctc
    14221 gttggtcggt ttgctgacca ttgccgtcta tctggtgctg gtgccattga tggtcttctt
    14281 cctgctgaaa gacaaagagc agatgctgaa cgccgttcgc cgggtgctgc cgcgcaaccg
    14341 tggactggca ggacaggtgt ggaaggagat gaatcaacaa atcaccaact atatccgcgg
    14401 caaagtgctg gagatgatcg tggtagggat cgccacctgg ctggggttct tgctctttgg
    14461 gctgaactat tcgctgctgc tggcggtgct ggtcggcttc tcggtactta ttccgtacat
    14521 tggcgcattt gtggtgacca ttccggtggt tggcgtggcg ctattccagt ttggtgctgg
    14581 cacggaattc tggagctgct tcgcggtgta tctgattatt caggcgctgg acggcaacct
    14641 gttagtaccg gtgttgttct ccgaagcggt taacctgcat cctctggtga ttattttatc
    14701 ggtggtgatc ttcggtggtt tgtggggatt ctggggcgta ttcttcgcta ttccattggc
    14761 gacgctgatc aaagccgtga ttcacgcctg gcctgatggg caaatcgcgc aagactgacc
    14821 ttcagcgaaa aaattttaaa cgagtgttgc agggcagtga gaagatttga gatgtgctgg
    14881 ccgcaagcgc agccagcacg gaatggagca aagtaatcag gcgtgttctt tcagccagtt
    14941 cagcacaacg tcgtggtgat tgctggtttt gaaatcgtca aagacatgtt cgattttgcc
    15001 atcagcgtca atcaggaagc tgatgcgatg aataccatcg taggttttgc ccatgaagga
    15061 cttttcaccc cagacgccga attgttcgca cacctggtgg tcctcatcag acaggagcgt
    15121 aaagttaagc agctcttttt ccgcaaaacg ggagagtttt tcgggtttat cggtgctgat
    15181 acccagcaca tcaacgcccg cttttttcaa ctcatccatg ttatcgcgta agccgcaggc
    15241 ctgtacggta cagccggggg tcatggcttt cgggtagaaa taaaccagaa cacgctgtcc
    15301 ctggaagtcg gtcaaattaa cttgttctcc gtcttgatcc ggcaagctaa atttcggtgc
    15361 gatatcaccg gctttcagtg gattcattac ttaactccat cctgttcatc atgttgggaa
    15421 taattgacga cgttaatact gccttgtgca ttgagttctg tacatagggc tttgaacgct
    15481 tgctcaatat ttgctgcgtc cgcagatgcg gggctgtggg cggttatctg aatatgcaac
    15541 tgcgcagccc gttcattttc agcaggttgc gtgcgcgaca ccagctccgc aatgttcata
    15601 tgatgcgcgt cgaaaagtgc tgtgaagcgt tcaattaaat gcggggagtc tgccacatcg
    15661 acctgaaccc agacagatgc tggcattggc ggacgcggac gcgccgtcgt gcgcttcatc
    15721 acgattaaaa gatccagctc ggcacctttc aacggtaacg ttgattcaat cagagtaatg
    15781 gcattccatg aaccggaaag cagcatgata aacgtgaact cttctcccag catcgccagg
    15841 cgactgtctt caatattaca gccgcaacta ctgacatgac gggtgatggt gttcactatt
    15901 ccagggcgat cggcacccaa cgcagtgatc accagataat gttgcgatga cagtgtcaaa
    15961 ctggttattc cttcaagggg tgagttgttc ttaaggaaag cataaaaaaa acatgcatac
    16021 aacaatcaga acggttctgt ctgcttgctt ttaatgccat accaaacgta ccattgagac
    16081 acttgtttgc acagaggatg gcccatgttc acgggaagta ttgtcgcgat tgttactccg
    16141 atggatgaaa aaggtaatgt ctgtcgggct agcttgaaaa aactgattga ttatcatgtc
    16201 gccagcggta cttcggcgat cgtttctgtt ggcaccactg gcgagtccgc taccttaaat
    16261 catgacgaac atgctgatgt ggtgatgatg acgctggagc tggctgacgg gcgcattccg
    16321 gtgattgccg ggaccggtgc taacgctact gcggaagcca ttagcctgac gcagcgcttc
    16381 aatgacagcg gtatcgtcgg ctgcctgacg gtaacccctt actacaatcg tccgtcgcaa
    16441 gaaggtttgt atcagcattt caaagccatc gctgaacata ctgacctgcc gcaaattctg
    16501 tataatgtgc cgtcccgtac tggctgcgat ctgctcccgg aaacggtggg ccgtctggcg
    16561 aaagtaaaaa atattatcgg aatcaaagag gcaacaggga acttaacgcg tgtaaaccag
    16621 atcaaagagc tggtttcaga tgattttgtt ctgctgagcg gcgatgatgc gagcgcgctg
    16681 gacttcatgc aattaggcgg tcatggggtt atttccgtta cggctaacgt cgcagcgcgt
    16741 gatatggccc agatgtgcaa actggcagca gaagggcatt ttgccgaggc acgcgttatt
    16801 aatcagcgtc tgatgccatt acacaacaaa ctatttgtcg aacccaatcc aatcccggtg
    16861 aaatgggcat gtaaggaact gggtcttgtg gcgaccgata cgctgcgcct gccaatgaca
    16921 ccaatcaccg acagtggtcg tgagacggtc agagcggcgc ttaagcatgc cggtttgctg
    16981 taaagtttag ggagatttga tggcttactc tgttcaaaag tcgcgcctgg caaaggttgc
    17041 gggtgtttcg cttgttttat tactcgctgc ctgtagttct gactcacgct ataagcgcca
    17101 ggtcagtggt gatgaagcct acctggaagc ggcaccgctt gcggagcttc atgccccggc
    17161 tggaatgatt ttgccggtga cctccggcga ttatgcaatc ccggtgacca acggtagtgg
    17221 tgctgtcggt aaggcgctgg acattcgtcc accagcccag ccgttggcac tggtttctgg
    17281 cgcgcgtacc cagttcacgg gcgataccgc ttcattgctg gtggaaaatg gtcgtggcaa
    17341 tactctgtgg ccgcaggtgg ttagcgtgct gcaggcgaaa aactacacca tcacccaacg
    17401 tgatgatgct ggtcagacac tgaccaccga ttgggtacaa tggaaccgtc tggacgaaga
    17461 cgagcagtat cgtggtcgtt atcaaatctc tgttaagccg cagggttatc agcaggcggt
    17521 tacggttaaa ctgctgaacc tggaacaggc gggcaaaccg gttgcagacg cggcgtccat
    17581 gcagcgttac agcacggaga tgatgaacgt tatttccgcc ggtctggata aatctgccac
    17641 tgacgccgcg aacgctgcgc aaaaccgtgc ctccaccact atggacgtac aaagtgcagc
    17701 tgatgacacc ggtttaccaa tgctggtcgt acgcgggccg ttcaatgtgg tttggcaacg
    17761 tctgccagct gcgctggaaa aagtgggcat gaaagtgacc gacagcaccc gttcgcaggg
    17821 caacatggcc gtaacttata agccgctgtc tgacagcgaa tggcacgaac tgggcgcgag
    17881 cgatccaggt ctggcatccg gtgactataa actgcaggtt ggcgatttag ataaccgcag
    17941 cagcctacag ttcatcgatc cgaaaggtca tactctgact cagagtcaga acgacgcgct
    18001 ggtagctgtc ttccaggctg cgtttagcaa gtaaaaatac agggctggag tcatccggcc
    18061 cttttttctg atatgatacg caaacgtgtg cgtcgacaga aaaacgcgat tttagcggta
    18121 attcgcacga aatttgtttg tcggacgtag gtcggataag gcgttcacgc cgcatccgac
    18181 aaaacatccg gcacaccaga cagcaaaaga ttttaaaacg ttaattcaca cccaggagtg
    18241 ataaagatgc aaaagcaagc tgagttgtat cgtggtaaag cgaaaaccgt atacagcacg
    18301 gaaaacccgg acctgttggt gctcgaattc cgcaatgata cgtcagcagg ggatggcgcg
    18361 cgcattgagc agtttgatcg caaaggtatg gtgaacaaca agttcaacta cttcattatg
    18421 agcaaactgg ctgaagcggg tatcccgact caaatggagc gtctgctctc cgataccgaa
    18481 tgtctggtga aaaagctgga tatggtgccg gttgagtgtg tcgtgcgtaa ccgtgctgct
    18541 ggctctctgg tgaaacgtct tggaatcgaa gaaggtattg agctgaaccc gccactgttc
    18601 gatctgttcc tgaaaaacga cgccatgcac gatccgatgg tcaacgaatc ttactgcgaa
    18661 acctttggct gggtgagcaa agagaatctg gcgcgtatga aagagctgac ctacaaagcg
    18721 aacgacgtgc tgaaaaaact gttcgatgat gcaggtctga ttctggtcga cttcaagctg
    18781 gagtttggtc tgtacaaagg cgaagtggtt ctgggcgatg agttctcccc ggacggcagc
    18841 cgcctgtggg acaaagaaac gctggagaaa atggacaaag accgtttccg acagagcctc
    18901 ggtggcctga tcgaagccta tgaagccgtt gcccgccgcc tgggtgtaca gctggactga
    18961 tttttctgtt catcatcttg ccgtgctgct ggcacggcaa gacaaccgct ctcataagat
    19021 gtgcattgaa cgctcattcc tccgccattt catcccgctt tcctgtggta atcttcctct
    19081 gaaccaccta cgatcatttc tataatgaat ataatgttga ggtatctatg cgttggcaag
    19141 ggcgacgtga aagtgacaat gttgaagaca ggcgcaacag ctctggtggt ccgtctatgg
    19201 gcggtcccgg ttttcgcctg ccaagcggta aaggcgggct gattttactg atagtcgtgc
    19261 tggttgcagg ctactatggc gttgatttaa ccgggttgat gaccggacag ccggtttccc
    19321 aacagcaatc aacgcggtca attagcccta atgaagacga agccgcaaaa ttcacctcgg
    19381 tgattctggc aaccacggaa gacacctggg gacagcaatt cgagaagatg ggtaaaacct
    19441 atcagcaacc gaagctggtc atgtaccgtg gaatgacgcg taccggctgc ggggcgggac
    19501 agtccattat ggggccattc tattgcccgg cggatggcac agtttatatc gatctctcct
    19561 tctatgatga catgaaagac aaacttggcg cggatggcga ttttgcccag gggtacgtta
    19621 tcgctcatga agtcggtcat catgtgcaga aactgctagg catcgagccg aaagttcgtc
    19681 aactgcaaca aaacgcgacg caggcggaag taaaccgctt atctgtgcgt atggaactcc
    19741 aggccgactg ttttgccggt gtatgggggc atagtatgca gcagcaaggt gttctggaaa
    19801 ctggcgatct ggaagaggcg ctgaacgcgg cgcaggccat cggcgatgac cgtttacaac
    19861 agcaaagtca ggggcgagta gtaccagaca gtttcactca tggcacttct cagcaacgct
    19921 acagctggtt taaacgtggt ttcgacagcg gcgatccggc acaatgcaat acttttggta
    19981 aaagcattta acttccgggg cagggatggc tgaactgact gcgcttcaca cattaacagc
    20041 gcaaatgaaa cgtgaaggga tccgccgctt gctggtgttg agcggggaag agcgttggtg
    20101 ttttgatcat gcgcttaagt tgcgtgatgc cttacctggc gactggctgt ggatttcgcc
    20161 gcagccagat gctgaaaacc actgttctcc ctcggcacta caaactttac ttgggcgcga
    20221 gttccggcat gcggtattcg acgcccgcca cggctttgat gccgctgcct ttgcggcact
    20281 tagcggaacg ttgaaagcgg gaagctggct ggtgttgtta ctccctgtat gggatgagtg
    20341 ggaaaaccaa cctgatgccg actcgctgcg ctggagtgat tgccctgacc ctattgcgac
    20401 gccgcatttt gtccagcatt tcaaacgcgt acttacggcg aataacgacg ctatcctctg
    20461 gcggcaaaac cagccgttct cgttggcgca ttttactccc cgtactgact ggcaccccgc
    20521 taccggcgca ccacaaccag aacaacagca actcttacag cagctactga ccatgccgcc
    20581 gggcgtggca gcggtaactg ctgcgcgtgg gcgcggtaag tcggcgttgg cagggcaact
    20641 catttctcgt attgcgggca gtgcgattgt caccgcgccc gcaaaagcgg caacggatgt
    20701 actggcacaa tttgcgggtg agaagtttcg ctttattgca ccggatgcct tgttagccag
    20761 cgatgagcaa gccgactggc tggtggtcga tgaagccgca gctatacctg cgccgttgtt
    20821 gtatcaactg gtatcgcgtt ttcctcgaac gttgttaacc actacggtgc agggctacga
    20881 aggcaccgga cgtggttttt tgctgaaatt ttgcgctcgc tttccgcatt tacacagttt
    20941 tgaactgcaa cggccgatcc gctgggcgca gggatgcccg ctggaaaaaa tggttagtga
    21001 ggcactggtg tttgacgatg aaaacttcac ccatacacca caaggcaata tcgttatttc
    21061 cgcatttgaa cagacgttat ggcgaagcga gccagaaacg ccgttaaagg tttatcaatt
    21121 attgtctggt gcgcactacc ggacttcgcc gctggattta cgccgcatga tggatgcacc
    21181 aggtcaacat tttttacagg cggctggcga aaacgagatt gccggagcgc tgtggctggt
    21241 ggatgagggt ggattatctc aagaactcag tcaggcggta tgggcgggtt atcgtcgtcc
    21301 acggggtaat ctggtggccc agtcgctggc ggcgcacggc agcaatccac tggcagcgac
    21361 attgcgtgga cggcgggtca gccggatagc agttcatccg gcgcgtcagc gggaaggcac
    21421 agggcggcaa cttattgctg gtgctttgca atatacgcag gacctcgact atctttcggt
    21481 gagttttggt tacaccgggg aattatggcg tttctggcaa cgctgcggtt ttgtgctggt
    21541 gcgaatgggt aatcatcgtg aagccagcag cggttgctat acggcgatgg cgctgttacc
    21601 gatgagtgat gcgggtaaac agctggctga acgtgagcat taccgtttac gtcgcgatgc
    21661 gcaagctctc gcgcagtgga atggcgaaat gcttcctgtt gatccactaa acgatgccgt
    21721 cctttctgac gacgactggc ttgaactggc cggttttgct ttcgctcatc gtccgctatt
    21781 aacgtcgtta ggttgcttaa tgcgtctgtt acaaaccagc gaactggcat taccggcgct
    21841 gcgtgggcgt ttacagaaaa acgccagtga cgcgcagtta tgtaccacac ttaaactttc
    21901 aggccgtaag ctgttactgg ttcgccagcg ggaagaggcc gcgcaggcgc tattcgcact
    21961 tgatgatgtt cgcactgagc gtctgcgcga tcgcataacg caatggcaat tttttcactg
    22021 actccttcaa ttttctccca tggtcattgt gagtcagcgg cgtaaaatta atgacatcag
    22081 ttaaggagtt cgccatgaaa catgaccatt ttgttgttca aagcccggat aaaccagcac
    22141 aacagttgtt actgcttttc cacggtgtcg gggataaccc ggtggcaatg ggggaaatag
    22201 gcagctggtt tgcgccgctg tttcctgatg cgctggtggt gagtgtcggt ggcgcggagc
    22261 cgagcggtaa tccggcgggg cgtcagtggt tttcggtgca gggtattacg gaagataatc
    22321 gccaggcgcg tgtggatgcc atcatgccga cgtttattga gacggtgcgc tactggcaga
    22381 aacaaagcgg agtaggggct aacgccacgg cgctcatcgg tttctctcaa ggcgcgatta
    22441 tggcgctgga gagcattaaa gccgaaccgg gccttgcttc gcgcgtcatt gccttcaacg
    22501 gacgctacgc gagcctgccg gaaactgcgt ctacagccac gacaattcac ttgattcatg
    22561 gcggtgaaga tccggttatc gatctggcgc atgcggttgc ggcgcaggaa gcgttaatca
    22621 gtgccggtgg tgacgtgacg ctggatatcg tggaggattt aggccatgcg atcgacaatc
    22681 gcagtatgca atttgccctg gatcacttgc gttataccat tccgaaacat tatttcgacg
    22741 aagcgctaag cggcggtaag cctggcgatg atgatgtgat tgaaatgatg taataatagc
    22801 cgggtaggcc tgataagacg cgtgagcgcc gcatcaggca tcgtttacca ttgccggatg
    22861 cggcgttaac gccttatccg gccacttagc ttacttcttc ggttgatcct ttttcggcca
    22921 gtcgtcgtca tcgtcccact tatcgttgaa atcacgatgc gggggaagct ccggcttgtt
    22981 ggcgagaaat ttcttatggt cgacgcgctt gagatcttta atcacgttca gcaggacgcc
    23041 taccaaaaag acaatcacca gaatccacca atatttagcc agccagtcca tgcttatttc
    23101 ctcttgcaga accactcatc aggcgacgag ctgttccatg atacgttgat acatacgggc
    23161 aagtagctgc aggtcggcag cgttcacaca ttcattaatt ttatgaatcg tggcattgac
    23221 cggcccgagt tccaccacct gcgcccccat gcgggcaata aagcgcccgt cggacgttcc
    23281 gcctgtggtc agtagctgcg gtttaatttc attatagtgc tcaaccgcgt taacgaccgc
    23341 atccaccagt ttaccgcgcg cggtcaaaaa tggctgcccg gaaagccacc aatccaccgt
    23401 atagcgcagt tgatgttttt caagcagggc aagcacctgc gctttgatca tctcatccgt
    23461 cagttcggtg ctaaagcgga agttaaactg cacaaacagt tcacctggaa taacgttgtt
    23521 actgcccgtt cctgcctgaa tattggcaat ctgcatactg gtcgccggga agaattcatt
    23581 accctgatcc cactcaatag ccactaattc attaatgaaa ggtgctgcgc gatgtaccgg
    23641 attgtcagcc agatgtgggt aggcaacatg cccctgaacg ccatgaatgg taaggttgca
    23701 ggttaacgat ccgcgacgac catttttcac cacatcacct accacttcga tactcgacgg
    23761 ttcgccaacc agacagtaat cgagacgctc attacgcgcc attaacgctt cgacgacttt
    23821 taccgtaccg ttgtgggcac tagcttcttc atcagaggtg atcagaaatg ccagtcgccc
    23881 cgtatggttg ggatgttgtg cgacaaagcg ttctgccgct accaccatcg ccgccagcga
    23941 gcctttcata tctgccgcac cgcgcccgaa taacatgccg tcacgaatgg ttggttcaaa
    24001 tggcggattg atccaacggt cggcgtcgcc aggcggcacc acgtcggtat gcccggcaaa
    24061 ggctaacgtt tcaccctgcc cacgccatgc ccaaaaattc tgcgtatcgg caaagtccat
    24121 gcgttcaacg gtaaaaccga tcgcctgcaa acgttcaatc aacaaagcct ggcatcctgc
    24181 gtcatcagga ctcagggaag ggcggcgaat aagctgttgt gtcagctcaa taaccgggca
    24241 cgacatagac tacacctcat ggaaaaattg ctgataactg gattcactga aacccagcag
    24301 cataggctta ccaggcgcgc agagcaatgg acgtttgata attgcaggca tttcagtcat
    24361 taatgtcgcc gcagaggccg catcggtgat tttattgcgg gtggtttcgt ccagtttacg
    24421 ccaggttgta ccacgggtgt tgagtaacgc ttcccagcct aattcgttga taaaaccgtt
    24481 caataattcg ctgtccaatc catcgacgcg gtaatcatga aaacgatagt cgatgttatt
    24541 ggcttccagc caacggcgag cctttttaat ggtgtcacaa tttttgatgc cgtaaagtgt
    24601 aaccataatg aatccttttt cgatttatga gaataaatac tcatttaagg gtgaaaacat
    24661 tttgcctgca a
//
LOCUS       DAZFJL010000070        24519 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFJL010000070 DAZFJL010000000
VERSION     DAZFJL010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.070"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            91..1383
                     /locus_tag="ACHJ8C_002693"
     CDS             91..1383
                     /locus_tag="ACHJ8C_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5552530.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKNNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLDEPKNWQQLLDVAQKMNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRNLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLISELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            1404..2285
                     /locus_tag="ACHJ8C_002694"
     CDS             1404..2285
                     /locus_tag="ACHJ8C_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO5552531.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIEATFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            2272..3114
                     /locus_tag="ACHJ8C_002695"
     CDS             2272..3114
                     /locus_tag="ACHJ8C_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO5552532.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            3145..4197
                     /locus_tag="ACHJ8C_002696"
     CDS             3145..4197
                     /locus_tag="ACHJ8C_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO5552533.1"
                     /translation="MKKLVATAPRVAALVEYEERAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFINDDFNGEWQMFTPRPANAPRGIEFGKFQLGNMVVGDIIECGSEVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPITRRCDIARRHGADFCLNPIGADVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            4214..5002
                     /locus_tag="ACHJ8C_002697"
     CDS             4214..5002
                     /locus_tag="ACHJ8C_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO5552534.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAENPAQAYRDSLAWLRTC"
     gene            5012..6067
                     /locus_tag="ACHJ8C_002698"
     CDS             5012..6067
                     /locus_tag="ACHJ8C_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO5552535.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADNVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTAEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVTAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            6064..8331
                     /gene="ycjT"
                     /locus_tag="ACHJ8C_002699"
                     /pseudo
     CDS             6064..8331
                     /gene="ycjT"
                     /locus_tag="ACHJ8C_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
     gene            8328..8987
                     /gene="pgmB"
                     /locus_tag="ACHJ8C_002700"
     CDS             8328..8987
                     /gene="pgmB"
                     /locus_tag="ACHJ8C_002700"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO5552536.1"
                     /translation="MKLQGVIFDLDGVITDSAHLHFKAWQQIAAEIGISIDVQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     TDLRAQQIPVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIEAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            9001..10083
                     /locus_tag="ACHJ8C_002701"
                     /pseudo
     CDS             9001..10083
                     /locus_tag="ACHJ8C_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            10128..11033
                     /gene="ompG"
                     /locus_tag="ACHJ8C_002702"
     CDS             10128..11033
                     /gene="ompG"
                     /locus_tag="ACHJ8C_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO5552537.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGLWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(11084..12082)
                     /locus_tag="ACHJ8C_002703"
     CDS             complement(11084..12082)
                     /locus_tag="ACHJ8C_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5552538.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNLAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYSHV
                     YSVDTDNYGDSIALTNALIESGHKNIACLHAPLDVHVSVDRVNGYKQSLATHNIAVRD
                     EWIVDGGYTHETALKAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFQLIAGKPSPQNITVATHMSLK"
     gene            12238..13635
                     /locus_tag="ACHJ8C_002704"
     CDS             12238..13635
                     /locus_tag="ACHJ8C_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO5552539.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     SIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            13632..14693
                     /locus_tag="ACHJ8C_002705"
     CDS             13632..14693
                     /locus_tag="ACHJ8C_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO5552540.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            14841..16382
                     /gene="tyrR"
                     /locus_tag="ACHJ8C_002706"
     CDS             14841..16382
                     /gene="tyrR"
                     /locus_tag="ACHJ8C_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO5552541.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(16426..16932)
                     /gene="tpx"
                     /locus_tag="ACHJ8C_002707"
     CDS             complement(16426..16932)
                     /gene="tpx"
                     /locus_tag="ACHJ8C_002707"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO5552542.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            17051..18016
                     /gene="ycjG"
                     /locus_tag="ACHJ8C_002708"
     CDS             17051..18016
                     /gene="ycjG"
                     /locus_tag="ACHJ8C_002708"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO5552543.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVTTAQTIVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(17991..18719)
                     /gene="mpaA"
                     /locus_tag="ACHJ8C_002709"
     CDS             complement(17991..18719)
                     /gene="mpaA"
                     /locus_tag="ACHJ8C_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO5552544.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASHESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(19010..19648)
                     /locus_tag="ACHJ8C_002710"
     CDS             complement(19010..19648)
                     /locus_tag="ACHJ8C_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5552545.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(19719..20639)
                     /locus_tag="ACHJ8C_002711"
     CDS             complement(19719..20639)
                     /locus_tag="ACHJ8C_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5552546.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSGEYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSQAGSKWMSDDLYDRASSKDKRYHIV
                     EGANHMDLYDGKVYVAEAISVLAPFFEETL"
     gene            20777..21676
                     /gene="pgrR"
                     /locus_tag="ACHJ8C_002712"
     CDS             20777..21676
                     /gene="pgrR"
                     /locus_tag="ACHJ8C_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO5552547.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            22013..23626
                     /gene="mppA"
                     /locus_tag="ACHJ8C_002713"
     CDS             22013..23626
                     /gene="mppA"
                     /locus_tag="ACHJ8C_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO5552548.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIIPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKAAPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGSQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(23677..>24519)
                     /gene="ynaI"
                     /locus_tag="ACHJ8C_002714"
     CDS             complement(23677..>24519)
                     /gene="ynaI"
                     /locus_tag="ACHJ8C_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO5552549.1"
                     /translation="VIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFEKQQIK
                     KGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILS
                     NFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKIKTFDNRPLYVPNSLFSS
                     ISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQF
                     ADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPP
                     DQGR"
BASE COUNT         5990 a         5849 c         6572 g         6108 t
ORIGIN      
        1 gggatctgat tagtgaagtt gatatattgg gtaatgaaat aacactgcat ccctggcagg
       61 ttatgtggat taaataaaaa ggaacatttc atgattaaat caaaaatcgt gctgttatca
      121 gcactggttt cctgcgccct gatttcaggc tgtaaagaag aaaataaaaa taatgtatcc
      181 atcgaattta tgcattcttc ggtggagcag gagcgccagg ccgtcatcag caaattgatt
      241 gcccgttttg aaaaagaaaa cccaggtatc accgtaaaac aagtgcccgt ggaagaagac
      301 gcctataaca ccaaagtcat taccctttca cgtagcggtt cgctgccgga agtgatcgaa
      361 accagccatg actacgccaa agtgatggac aaagagcagc ttatcgatcg taaagcggtt
      421 gccacggtca tcagcaacgt cggtgaaggc gcgttctacg atggcgtact gcggattgtg
      481 cgcaccgaag atggcagcgc ctggaccggt gttcctgtca gcgcatggat tggcgggatc
      541 tggtatcgca aagatgtgct ggcaaaagcc gggctggatg agccgaaaaa ctggcaacag
      601 ttgctggatg tcgcacagaa aatgaacgat ccggcgaata aaaaatacgg tattgcgctg
      661 cctacagcag aaagcgtgtt gacggaacag tctttctccc agtttgcgtt atccaatcag
      721 gctaacgtct ttaacgccga aggcaaaatc acccttgata caccagagat gatgcaggcg
      781 ctgacctatt accgcaacct tgctgccaac accatgccag gttctaacga catcatggaa
      841 gtgaaagacg cttttatgaa cggcaccgcg ccgatggcga tttactccac ctatatcctt
      901 ccggctgtga ttaaagaagg cgatccgaaa aacgtcggtt tcgtggtgcc aaccgagaaa
      961 aattctgcgg tctacggcat gttgacctcg ctgaccatta ccgccgggca aaaggccgaa
     1021 gagacggaag cagcagaaaa atttgtcacc tttatggagc aggcggacaa cattgccgac
     1081 tgggtgatga tgtcgccagg cgcggcgctg ccggtgaaca aagcggtggt gactaccgcc
     1141 acctggaaag acaacgacgt tattaaggcg ctgggagaat tgccgaatca gctaatcagc
     1201 gaactgccaa atattcaggt ctttggcgca gtaggggata aaaactttac ccgcatgggc
     1261 gatgtgacgg ggtctggcgt ggtgagttca atggtgcata acgtcaccgt ggggaaagcc
     1321 gatctccctg gcacgctgca agcgagccag aaaaagctgg atgaactgat cgaacagcac
     1381 taaacccagg acaggaatcc gcaatgaaca ggcttttttc aggtcgttcc gatatgcctt
     1441 ttgcactgct gcttctcgcg cccagcttat tactgctggg cggtctggtg gcgtggccga
     1501 tggtgtcgaa tatcgaaatc agttttttac gtctgccgct taatcccaat atcgaggcaa
     1561 catttgttgg ggtgagcaac tatgtgcgta ttctctccga tccgggcttc tggcactcgc
     1621 tgtggatgac ggtctggtat accgcgcttg tggtggcggg cagcaccgtg ctcgggctgg
     1681 cagtggcgat gtttttcaac cgcgagttcc gcctgcgtaa aaccgcgcgt tcgctggtga
     1741 tcctctccta cgtaacgcca tccatttcgc tggtgtttgc ctggaaatac atgttcaaca
     1801 acggctacgg cattgtgaac tacctcggtg tcgatcttct gcatctctat gagcaggcac
     1861 cgctgtggtt cgataatccg ggcagtagct ttgtgctggt ggtgctgttc gccatctggc
     1921 gctacttccc gtatgccttt atctcgtttc tggcgatttt gcagaccatc gacaaatcgc
     1981 tgtacgaagc ggcagagatg gatggcgcga atgcctggca gcggtttcgc atcgtcacgc
     2041 tgcccgcaat tatgccggtc ctggcgacgg tggtgaccct gcgcaccatc tggatgttct
     2101 acatgttcgc ggatgtttat ctgctgacca ccaaggtcga tattcttggt gtctatctct
     2161 acaaaaccgc ctttgccttt aatgacttag ggaaagcggc ggcgatctcg gtggtgctct
     2221 tcatcattat tttcgctgtc attctgctga ccaggaaacg ggtgaatctc aatggcaaca
     2281 aataaacgca cactcagtcg aatcggtttt tactgcgggc tggcgctgtt tctcatcatc
     2341 acgctgtttc cgttttttgt gatgctgatg acctcgttca agagcgcaaa agaggcgatc
     2401 tcgctgcatc ctacgctgct gccacagcag tggacgctgg agcattacgt cgacattttt
     2461 aatccggtga ttttcccgtt tgtcgactac ttccgtaaca gcatggtggt gtcggtggtt
     2521 tcctccgtgg tggcggtatt tctcggcatt ctcggagctt acgcgctttc ccgcctgcgc
     2581 tttaaaggcc ggatgaccat caacgccagc ttttacacgg tgtatatgtt ctctggcatt
     2641 ttgctggtgg tgccgctgtt caaaatcatc accgcgcttg gtatttacga caccgagatg
     2701 gcgctgatca ttaccatggt gacgcaaacc ctgcccaccg ccgtgtttat gctgaaaagc
     2761 tacttcgaca ccatccctga tgagattgaa gaagcggcga tgatggatgg cctcaaccgt
     2821 ctgcaaatca tcttccgtat caccgtgccc ctggcgatgt ccgggctgat atccgtattc
     2881 gtctactgct ttatggtggc atggaacgac tatctgtttg cgtcgatttt cctctccagc
     2941 gccagcaatt tcaccttacc agtcggcctg aacgcgctgt tcagtacgcc tgattacatc
     3001 tggggacgga tgatggcggc ctcgctggtg accgcattgc cggtggtgat tatgtatgcg
     3061 ctttcggagc gttttattaa gagtggtttg accgccggtg gcgtgaaggg ctaaagcgac
     3121 caatttcttt acaaggagtt ttacatgaaa aagttagtag ccacagcacc gcgtgtagct
     3181 gcgctggttg agtatgaaga gcgggcgatt ttagccaatg aagtgaagat ccgcgtgcga
     3241 ttcggcgcgc cgaaacacgg gacggaagtg gttgatttcc gcgccgccag cccgtttatt
     3301 aatgacgact ttaatggcga atggcagatg ttcaccccgc gtccggcaaa tgcgccgcgc
     3361 ggcattgagt ttggcaagtt ccagcttggc aacatggtgg ttggcgacat tatcgagtgt
     3421 ggcagcgagg ttaccgacta cgcggtgggc gacagcgtat gcggctacgg cccgctttcc
     3481 gaaacggtca tcattaacgc agtgaataac tacaaactgc gcaagatgcc gcaaggtagc
     3541 tcgtggaaaa acgccgtctg ttacgacccg gcgcagtttg ccatgagcgg cgtgcgcgat
     3601 gccaacgtgc gcgtgggcga ttttgtggtg gtggtcgggc ttggcgcgat cggtcaaatt
     3661 gccattcaac tggcaaaacg cgctggcgcg tcggtggtga ttggcgtcga tcctattacc
     3721 cgtcgttgtg atattgcccg tcgccacggt gcggatttct gtcttaaccc cattggcgct
     3781 gacgtaggta aagagatcaa aacgctgacc ggcaagcagg gtgccgatgt gattatcgaa
     3841 accagtggct atgccgacgc gctgcaatcg gcactccgcg gtctggccta tggcggcacc
     3901 atctcctatg tcgcgtttgc caaaccgttt gccgaaggtt ttaacctcgg acgcgaagcc
     3961 catttcaata acgccaaaat tgttttctcc cgtgcgtgca gcgagccgaa cccggattat
     4021 ccgcgctgga gccgtaagcg tattgaagaa acctgctggg aactgctgat gaacggttat
     4081 ctcaattgcg aagatttaat cgatccggtg gtgacctttg ccaacagccc ggaaagctac
     4141 atgcagtatg tcgaccagca tccggaacag agcatcaaaa tgggcgttac gttttaatca
     4201 aaggaatgaa aaaatgaaaa tcggcacaca gaatcaggcg ttctttccgg aaaacattct
     4261 ggagaaattt cgttatatca aagagatggg cttcgatggt tttgagattg acggcaaact
     4321 gctggtcaac aacatcgaag aagtcaaagc ggcgatcaaa gaaaccggtt taccggtgac
     4381 caccgcctgc ggtggctatg acggttggat tggcgacttt atcgaagagc gtcgacttaa
     4441 tggcttaaag cagatcgaac gcattctcga agcgctggca gaagtgggcg gtaaaggtat
     4501 cgtcgttccg gctgcgtggg gcatgtttac cttccgctta ccgccgatga cctcgccgcg
     4561 cagccttgac ggcgatcgta aaatggtgag tgattccctg cgcgtactgg aacaggtcgc
     4621 cgcgcggact ggaactgtgg tgtatctcga accgttaaac cgctatcagg atcatatgat
     4681 caacaccctc gccgatgctc gtcgttacat cgtcgaaaac aatcttaaac atgtgcagat
     4741 tatcggcgat ttctatcaca tgaatatcga agaagataac ctggcgcagg cgttgcacga
     4801 caaccgcgac ttgctcggtc atgtgcatat tgccgataac catcgctacc agccgggcag
     4861 cggcaccctc gatttccacg cgctgtttga acagctgcgc gccgataact atcagggcta
     4921 tgtggtgtat gaagggcgta tccgggcaga aaatcctgcc caggcgtacc gtgattcgtt
     4981 ggcctggttg cgcacctgct aagaggtctt tgtgaaaagt gcaatgacaa gctctccgct
     5041 gcgtgtcgcg ataatcggcg caggccaggt ggcggataat gttcatgctt cgtactactg
     5101 cacccgcaac gatctggaac tggtggctgt ctgtgacagc cgcctttccc aggcgcaggc
     5161 gctggcagaa aaatacggga atgcatccgt gtgggacgat ccgcaggcca tgctgctggc
     5221 ggtgaaacct gatgtggtta gcgtctgctc acctaaccgt tttcattacg aacataccct
     5281 gatggcgctg gaagcgggct gccatgtgat gtgcgaaaaa ccgcccgcca tgacggcaga
     5341 acaggcgcgg gaaatgtgcg ataccgcgcg caaacagggc aaggtgctgg cctacgactt
     5401 tcaccatcgt tttgcactcg atacgcaaca gctgcgtgaa caggtgacca acggcgtttt
     5461 gggagagatt tacgttacca ccgcccgcgc cctgcgtcgt tgcggtgttc ccggctgggg
     5521 tgtctttact aataaagaat tgcagggcgg aggcccgctg atcgacatcg gcattcatat
     5581 gctggatgcg gcgatgtatg tgctgggttt tccagcggtg aaaagcgtga ctgcgcatag
     5641 ctttcaaaag atcggcacgc aaaagagctg cggtcaattt ggcgagtggg acccggcaac
     5701 ctacagcgtc gaagattcgc tgtttggcac cattgaattt cataacggcg gcattctgtg
     5761 gcttgagacc tcatttgcac tcaacatccg cgaacagtcg attatgaacg tcagcttttg
     5821 tggtgataaa gccggggcga cgctgtttcc agcacatatc tacaccgata acaacggtga
     5881 gttaatgacg ctgatgcaac gggaaatggc agacgacaac cgtcatctgc gcagtatgga
     5941 agcctttatc aaccacgtac aaggtaagcc cgtgatgata gccgacgccg agcagggata
     6001 catcatccag caactggtgg cggcgttgta tcaatccgca gaaacaggga cgcgtgtgga
     6061 attatgatca ggccagtaac gttaacggaa ccccatttca gccagcatac cctgaacaag
     6121 tatgcttcgc tgatggcgca ggggaacggc tatcttgggc ttcgcgccag ccatgaagaa
     6181 gattacacgc gccagacgcg cggcatgtat ctggcagggc tttatcatcg ggcgggtaaa
     6241 ggtgaaatca acgaactggt gaacctgcct gatgtcgtgg ggatggagat tgccataaat
     6301 ggtgaggttt tctcgttatc ccgcgaagcc tggcagcgtg agcttgactt tgccagtggc
     6361 gaattacgcc gcaacgttgt ctggtgtacc agtaacggcg cgcgttacac catcgtcagc
     6421 cgtcgctttg tttcggcaga ccaactgccg ctcattgcgc tggaaatcac tattacgcca
     6481 ctggacgctg acgcgttagt gctgatttca acaggtattg acgccacgca aaccaaccac
     6541 ggtcgccaac atctcgacga aacccagatg cgggtatttg gtcagcatct gatgcagggg
     6601 atctacacca cccaggatgg gcgcagtgat gtggccatca gctgttgctg taaggtgagc
     6661 ggtgatgtgc agcaatgcta taccgccaaa gagcgccgtt tactgcaaca tacctgcgcg
     6721 cagcttcacg caggtgagac gttgacgctg caaaaacggg tgtggatcga ctagcgggac
     6781 gacagacaag ccgctttaga cgaatggggc agtgcgtcgc ttcgtcagct tgaaatgtgc
     6841 gcgcagcaga gttacgacca acttcttgca gcatcaacag aaaactggcg tcaatggtgg
     6901 cagaaacgtc gtatcacggt aaacggcggc gatgcgcacg atcagcaagc gttagattat
     6961 gcgctttatc acctgcgcat catgacgcct gcccacgatg agcgcagcag cattgcggcg
     7021 aaaggtttaa ccggcgaagg ctacaaaggc cacgttttct gggatacaga agtatttttg
     7081 ctaccgttcc atctgtttag cgatccgacg gttgcccgaa gtttactgcg ttatcgctgg
     7141 cacaacttgc ctggcgcgca ggagaaagcg cgacgcaacg gctggcaggg cgctctgttt
     7201 ccgtgggaaa gcgcgcgtag cggcgaagaa gagacgccgg aatttgctgc cattaacatt
     7261 cgtaccgggc tgcggcaaaa agtggcctct gcgcaggcgg aacatcatct ggtggccgat
     7321 atcgcctggg cggttattca atactggcgg accacggggg atgaaagttt cattgctcat
     7381 gaaggcatgg cgctacttct ggagacggca aagttctgga ttagccgcgc ggtgagggtt
     7441 aacgaccgtc tggaaattca tgatgttatt gggccagacg aatataccga acatgtcaat
     7501 aataacgcct tcaccagcta tatggcgtat tacaacgtcc agcaggcgct gaatatcgct
     7561 cgtcagtttg gctgtagcga cgacgcgttt attcatcgtg cagaaatgta cctcaaagaa
     7621 ctgcggctgc cagaaattca gcccgacggc gttttgcctc aggatgattc gtttatggct
     7681 aagtcggtga ttaatctggc gaaatacaaa gaggcggcgg ggaagcaaac cattctgctg
     7741 gattattcac gcgcagaagt gaacgagatg cagatcctca aacaagctga tgtggtgatg
     7801 ctcaattaca tgctgccgga gcagttctca gcggcatcgt gtcttgccaa tctgcaattt
     7861 tatgaaccgc gcactattca cgactcgtca ttaagtaaag caatccacgg cattgttgcc
     7921 gcacgctgtg gcctgttggc gcaaagttat cagttctggc gggaggggac tgaaatcgat
     7981 cttggtgctg atccgcatag ctgtgatgac ggtatccacg ctgccgcaac tggcgctatt
     8041 tggctggggg cgattcaggg ttttgccggg gtgagcgtgc gtgacggtga attacatctc
     8101 aatccggcgt tacctgagca gtggcaacag ttgtctttcc ctctgttctg gcagggctgc
     8161 gaattacagg tcactctcga cgcgcagcgt attgcgattc gaacttctgc gcccgtttca
     8221 ctgcgtttga acgggcagct tatatctgtg gctgaagaat ctgttttctg tttgggtgat
     8281 tttattttgc ccttcaatgg gaccgccacc acgcatcagg aggatgaatg aaactgcaag
     8341 gggtaatttt cgatctggat ggagtaatca cagatagcgc acatctgcat ttcaaggcgt
     8401 ggcagcagat tgccgctgaa attggcatca gcattgatgt gcagtttaac gaatccctaa
     8461 aagggatcag ccgcgatgag tctctgcggc gcattctgca acacgggggc aaagagggcg
     8521 actttaaccc gcaggagagg gcgcaactgg cgtatcgcaa aaatctgctc tacgtccact
     8581 cactgcgcga actgacggtt aatgctgtgc tgcccggcat tcgctctttg ctgacagatc
     8641 tccgtgcaca acagatcccg gtagggctgg cttctgtctc cctcaatgcg ccgacgattt
     8701 tagatgcact ggagctgcgt gagtttttca ccttctgcgc ggacgcttcc caactcaaaa
     8761 attcgaaacc ggacccggaa atctttctcg ccgcctgtgc agggctgggc gtgccgccac
     8821 aggcatgtat cggcattgaa gatgcgcagg cgggcattga agccattaac gccagcggta
     8881 tgcgctcggt ggggatcggc gcgggcttaa ctggggcgca attattgttg ccctcaacgg
     8941 actccctgac ctggccgcgg ttatcggcct tctggcaaaa cgtatagcaa aggaatcaac
     9001 atggcacagc tttcgttaca acatattcaa aaaatctacg ataaccaggt gcatgtggtg
     9061 aaggacttca acctggaaat tgccgataaa gagttcattg tatttgtcgg tccgtcaggc
     9121 tgcggtaaat cgactacgct gcgcatgatt gccgggcttg agtagatcag cggcggtgat
     9181 ctgttgatcg acggcaaacg aatgaatgac gttccagcca aagcacgcaa tatcgcgatg
     9241 gtgttccaga actacgcgct gtatccgcat atgacggtct acgacaacat ggcgtttggc
     9301 ctgaagatgc aaaaaatcgc cagagaggtg attgatgagc gggtgaactg ggcggcgcaa
     9361 attctcggcc tgcgtgagta cctgaaacgc aagccgggcg cgctttccgg cgggcaacgt
     9421 cagcgagtgg cgcttgggcg ggcgattgtg cgcgaagcgg gcgtgttttt aatggatgag
     9481 ccgctctcta acctggatgc caagctgcgc gtgcaaatgc gcgcagagat cagcaagctg
     9541 catcagaaac tgaacaccac catgatctac gtgacccacg atcagaccga agcgatgacc
     9601 atggcgacgc ggattgtcat tatgaaggat gggattgttc agcaggtcgg tgcgccgaaa
     9661 acggtttata accaacctgc gaatatgttt gttgccggat ttattggatc accagcgatg
     9721 aattttattc gcggcacgat cgatggcgat aaattcgtta cggaaacgct taaattaacc
     9781 atacccgaag agaaattagc ggttctgaaa acacaggaaa gtttgcataa gcccatcgta
     9841 atgggaatac gcccggaaga tattcatccg gacgcgcaag aggaaaataa catttccgcc
     9901 aaaattagcg tggcggaatt aaccggtgcg gaatttatgc tctacaccac ggttgggggg
     9961 cacgagttag tggtccgtgc tggtgcgtta aatgattatc atgcaggaga aaatatcact
    10021 attcattttg atatgactaa atgtcatttc tttgatgcag aaacggaaat agcaatttgc
    10081 taaatacagg gggaaggcat tctcccagga taatacaagg aacaataatg aaaaagttat
    10141 taccctgtac cgcactggtg atgtgtgcgg gaatggcctg cgcacaggcc gaggaaaaga
    10201 acgactggca ctttaatatc ggtgcgatgt acgaaataga aaacgtcgaa ggttatggcg
    10261 aagatatgga tgggctggcg gagccttctg tctattttaa tgccgccaac gggctgtgga
    10321 gaatttctct ggcctattat caggaagggc cggtagatta tagcgcgggt aaacgtggaa
    10381 cgtggtttga tcgcccggag ctggaggtac attatcagtt cctcgaaagc gatgatttca
    10441 gtttcggtct gactggtggt ttccgtaatt atggttatca ctacgttgat gaaccgggta
    10501 aagacacggc gaatatgcag cgctggaaaa ttgcgccaga ctgggatgtg aaactgactg
    10561 acgatttacg tttcaacggt tggctgtcga tgtataaatt tgccaacgat ctgaatacga
    10621 ccggttatgc tgatacccgt gtcgaaacgg aaacgggtct gcaatatacc ttcaacgaaa
    10681 cggttgcctt gcgagtgaac tattatctcg agcgcggttt caatatggat gatagccgca
    10741 ataacggtga gttttccacg caagaaattc gcgcctattt gccgctgacg ctcggcaacc
    10801 actcggtgac gccgtatacg cgcattgggc tggatcgctg gagtaactgg gactggcagg
    10861 atgatattga acgtgaaggc catgatttta accgtgtggg tttattttac ggttatgatt
    10921 tccagaatgg tctttccgtt tcgctggaat acgcgtttga gtggcaggat cacgacgaag
    10981 gcgacagcga taaattccat tatgcgggtg ttggggtgaa ttactcgttc tgataatgtg
    11041 ctaaattgcc ggatgcgggg cgagcgccaa atccggcata tatttatttc aacgacatat
    11101 gcgtagcaac ggtaatattt tgtggtgacg gttttccggc aattagctgg aataataact
    11161 cgcagctttg ttgacctaac tcctgcgtcg gaacatcgat gccgcctggt gcaggcgtta
    11221 atataaacga cagcgtttca ttactataac ccaccaccgc taactgctgc ggaatagcaa
    11281 tgtttttctc tgccgccgca cgataaatac tcattaattt caggctgtca gtagcaaaca
    11341 ctgcctcagg caacggtgac tggcttaata attcccgtgc tgcttttaat gctgtctcat
    11401 gggtataacc gccgtcaaca atccattcat cacgcactgc aatattatgc gtagccaggc
    11461 tttgcttata accatttacc cgatccactg aaacatgaac atcaagcggc gcatgcaggc
    11521 aggcaatatt cttatgcccg ctttcaatta acgcattggt cagcgcaata ctgtcgccat
    11581 aattatcggt atcgacggaa taaacatggc tgtattgacc ttcaactttg ccaattacca
    11641 ccaccggaat atcatattta tcgagttggg caaaaaatga ctcatccgct ggcgaactta
    11701 gcataataat gcctttaatc attttctgct taattttgct ttcgcatttt tgtaagtctt
    11761 ctgccaggtt gtgcgacgtc tgtaatatca cgtcgaaacc ttcttcttcc gctttggcgg
    11821 tgatggcatg taaaacttcc gagaaaaacg gattacccgc cgtagttttg gtcgaacggg
    11881 tagaaatcac cataatggca tcaaaacccg aagaggtcag cgcgcgtgcc agcttgtttg
    11941 gttgatattg taattcttca atggcccgta acactttttc gcgcgcttcc ggggagatat
    12001 tggtttgctt attcagcacg cgtgatacgg tggattttga tacgcctgca accctggcaa
    12061 tatcataaat agtaggggac ataggtcagg aactccgtcc gaacgtgaat gctgcacatc
    12121 ttatggagtt cgcccgtcga tgacaacacg ggtagttgat aatcaatggc ctggccccca
    12181 cattcatatc cttacgaacg attttttttc tggtcttcag agcagcacag gacagcaatg
    12241 aagcgactta aaaatgaact caatgcgctg gtgaatcggg gtgtcgacag acatctgcgc
    12301 ctcgctgtaa ccggacttag ccgcagcggc aaaacagcgt ttatcaccgc gatggttaat
    12361 cagttgctca gtattcatgc cggagcacgt ttgccgctat taagtgcggt gcgtgaagaa
    12421 cgcctgctgg gcgtaaaacg cattcctcag cgtgactttg gcattccgcg cttcacctat
    12481 gatgaagggt tggcgcagtt atatggcgat cctcccgcct ggccgacgcc tacgcgcggc
    12541 gtcagtgaaa ttcgcctggc gttacgtttt aaatcgaacg attcgctgct gcgccacttt
    12601 aaggacacct ccacgctgta tctggagatt gtggattacc ccggcgaatg gttgctcgac
    12661 ctgccgatgc tggcgcagga ctatttaagc tggtcgcgcc agatgacggg cttactcaat
    12721 ggtcagcgcg gcgaatggtc ggcgaaatgg cgaatgatgt gcgaagggct ggacccgtta
    12781 gcgcctgccg acgaaaatcg cctggcggat attgccgctg catggaccga ttatctccac
    12841 cactgcaaac agcaggggct gcactttatt cagccaggac gctttgtttt gcctggcgat
    12901 atggcaggag cgcccgcgct gcaattcttc ccatggccag atgtcgatgc ctggggcgag
    12961 tccaaactgg cgcaggccga taaacacacc aacgccggaa tgttgcgcga gcgttttaat
    13021 tattactgcg agaaagtggt gaaggggttc tataagaatc attttctgcg ctttgaccgc
    13081 cagattgtgc tggtggattg cctgcaacct ctcaacagtg ggccacaggc atttaatgat
    13141 atgcgtctgg cgctgacgca gctgatgcaa agtttccact acgggcagcg taccctgttc
    13201 cggcgtttgt tttcgccggt tatcgataag ctattgtttg ctgccactaa agcggaccat
    13261 gtgaccatcg atcagcacgc taatatggtt tcattgctgc aacaactgat tcaggatgcc
    13321 tggcaaaatg cggcgttcga agggatcagt atggactgtc tggggctggc gtctgttcag
    13381 gcgaccacca gcggaattat tgatgttaac ggtgagaaaa tcccggcgct gcgcggtaat
    13441 cgactcagcg atggcgcacc gctcactgtt tatcctggcg aagttcccgc acgtttgcca
    13501 ggtcaggcat tctgggataa gcaaggcttc cagtttgagg cgtttcgtcc gcaggtgatg
    13561 gatgtcgaca aaccgctacc gcatattcgt cttgatgctg cgctggaatt tttaatagga
    13621 gataaattgc gatgaccgaa ccgttaaaac cacgtattga tttcgacggt ccactggagg
    13681 tcgatcagaa tccaaaattc agggcgcagc agacctttga cgaaaatcag gcgcaaaatt
    13741 ttgccccagc cacgctcgat gaagcgcagg aagaagaggg gcaagttgaa gctgtgatgg
    13801 acgcagcgtt acgtccgaaa cgcagcctgt ggcgcaaaat ggtgatgggc gggctggctc
    13861 tgtttggcgc aagcgttgtc gggcagggcg tgcagtggac aatgaacgcc tggcaaaccc
    13921 aggactgggt ggcgctgggc ggatgtgccg ctggggcatt gattatcggc gctggcgtag
    13981 gttctgtggt aacagagtgg cggcgcttat ggcgcttgcg acagcgcgcc catgaacgcg
    14041 acgaagcgcg cgatttgttg cacagtcacg gcacgggtaa aggccgtgca ttctgcgaaa
    14101 aactggcgca gcaggcgggc atcgatcagt cacatccggc tctgcaacgc tggtatgcct
    14161 caatccatga aacgcaaaac gaccgtgaag tggtcagttt gtatgcgcat ttggtccagc
    14221 cagttttaga tgcccaggcg cggcgcgaaa tcagccgttc ggcagcggaa tcaacattga
    14281 tgattgcggt cagcccgctg gcgctggtgg atatggcatt tatcgcctgg cgcaatctgc
    14341 gtttgattaa tcgcatcgcc acgctgtatg gcattgaact ggggtattac agccgtttgc
    14401 gcctgtttaa gctggtattg ctgaatatcg ctttcgccgg agccagcgaa ctggtgcgcg
    14461 aagtggggat ggactggatg tcgcaagatc tcgctgcgcg tttgtctacc cgggcagctc
    14521 aggggattgg tgctggactt ctgacggcac gactggggat taaagctatg gagctttgcc
    14581 gcccgctgcc gtggattgac gatgacaaac ctcgcctcgg ggatttccgt cgtcagctta
    14641 tcggtcaggt gaaagagacg ctgcaaaaag gcaaaacgcc cagcgaaaaa taatgcaata
    14701 ccgggtgctg accggatatc tttacgccga agtgcccgtt tttccgtctt tgtgtcaatg
    14761 attgttgaca gaaaccttcc tgctatccaa atagtgtcat atcatcatat taattgttct
    14821 tttttcaggt gaaggttccc atgcgtctgg aagtcttttg tgaagaccga ctcggtctga
    14881 cccgcgaatt actcgatcta ctcgtgctaa gaggcattga tttacgcggt attgagattg
    14941 accccattgg gcgaatctac ctcaattttg ctgaactgga gtttgagagt ttcagcagtc
    15001 tgatggccga aatacgccgt attgcaggtg ttaccgatgt gcgtactgtc ccgtggatgc
    15061 cttccgaacg tgagcatctg gcgttgagcg cgttactgga ggcgttgcct gaaccagtgc
    15121 tctctgtcga tatgaaaagc aaagtggata tggcgaaccc ggcgagctgt cagctttttg
    15181 ggcaaaaatt ggatcgcctg cgcaaccata ccgccgcgca attgattaac ggctttaatt
    15241 ttttacgctg gctggaaagc gaaccgcaag attcgcataa cgagcatgtc gttattaatg
    15301 ggcagaattt cctgatggag attacgcctg tttatcttca ggatgaaaat gatcaacacg
    15361 tcctgaccgg tgcggtggtg atgctgcgat caacgattcg tatgggccgt cagttgcaaa
    15421 atgtcgccgc ccaggatgtc agcgccttca gtcaaattgt cgctgtcagc ccgaaaatga
    15481 agcatgttgt cgaacaggcg cagaaactgg cgatgctaag cgcgccgctg ctgattacgg
    15541 gtgataccgg caccggaaaa gatctctttg cgtatgcctg tcatcaggca agccccagag
    15601 cgagcaaacc ttacctggcg ctgaactgtg cgtctatacc ggaagatgcg gtcgagagtg
    15661 aactgtttgg tcatgctccg gaagggaaga aagggttctt tgagcaggcg aacggtggtt
    15721 cggtgctgtt ggatgaaata ggtgagatgt caccacggat gcaggcgaaa ttactgcgtt
    15781 tccttaatga tggcaccttc cgtcgggttg gcgaagacca tgaggtgcat gtcgatgtgc
    15841 gggtgatttg tgctacgcag aagaatctgg tcgaactggt gcaaaaaggc gtgttccgtg
    15901 aagatctcta ttatcgtctg aacgtgttga cgctcaatct gccgccgcta cgtgactgtc
    15961 cgcaggacat catgccgtta accgagctgt tcgtcgctcg ctttgccgac gagcagggcg
    16021 tgccgcgtcc gaaactggcc gctgacctga atactgtact tacgcgttat gcgtggccgg
    16081 gaaatgtgcg gcagttaaag aacgctatct atcgcgcact gacacaactg gacggttatg
    16141 agctgcgtcc acaggatatt ttgttgccgg attatgacgc cgcaacggta gccgtgggcg
    16201 aagatgcgat ggaaggttcg ctggacgaaa tcaccagccg ttttgaacgc tcggtattaa
    16261 cccagcttta tcgcaattat cccagcacgc gcaaactggc aaaacgtctc ggcgtttcac
    16321 ataccgcgat tgccaataag ttgcgggaat atggtctgag tcagaagaag aacgaagagt
    16381 aagcgtgaat atgcctgatg gtgcaacacc atcaggcata ttaacttatg ctttcagtac
    16441 agccagagct gcttcgtaat ccggctcggt ggtgatttca tccaccagct ggctgaaaat
    16501 cacattgtca ttttcatcaa taaccacaac ggcacgcgct gccagacctt tcagtgggcc
    16561 atcagcaatt gccacaccgt aggcttgcag gaattcagcg ttacggaaag tggagagggt
    16621 gataacgttg ttcagacctt ctgcgccgca gaaacgagac tgggcgaacg gcagatcggc
    16681 agagatacac agcacaacgg tgttgtcgat ctcggttgcc agttggttaa acttacgtac
    16741 tgatgcggcg caaacaccgg tatcaatact cgggaaaatg ttcagcactt tgcgtttacc
    16801 cgcaaactga ccgagggtga cgtcagacag atcttttgcc acgagagtaa aagtctgcgc
    16861 tttgctaccc gcctgcggga tggaattggc gactgtaacc gggttgccct ggaaatgaac
    16921 ggtttgtgac atgattatct ttcctgttta catatagtta acgtcacacc tagtttatgc
    16981 taactgtcaa taacacagca aacgctattt gcgcttaatc cgcagaccac cgcgacaaca
    17041 aggagtaaag atgagaaccg ttaaggtatt tgaggaggcc tggcccttac ataccccgtt
    17101 tgtgattgct cggggaagtc gcagtgaagc gcgcgtggtg gtggttgaac tggaagaaga
    17161 gggtattaaa ggcaccggcg aatgcacgcc gtatccgcgt tatggggaaa gtgatgcctc
    17221 ggtaatggcg caaattatga gcgtcgtgcc gcaattagag aaagggctga cacgggagga
    17281 gttgcaaaaa atcctccctg ccggcgcagc acgtaatgcg ctggattgtg cattgtggga
    17341 tctggcggcg cgaaaacagc agcaatcgct ggctgatttg atcggcataa cgcttcccga
    17401 gacagtcacc acggcacaga cgatagtcat cggtacgcct gatcagatgg ccaatagtgc
    17461 atcaacactc tggcaggcag gcgcgaaatt actgaaagtg aagctggata accatcttat
    17521 cagtgagcgg atggtggcaa ttcgcacagc tgtgcccgat gcgacgctga tcgttgatgc
    17581 aaatgaatcc tggcgtgcag aagggttggc ggcgcgttgc cagctattgg cagatttagg
    17641 cgttgcgatg cttgaacaac cgcttcctgc gcaggacgat gcggcgctgg agaattttat
    17701 tcatccgtta ccgatttgtg ctgatgaaag ttgtcatact cgtagcaatc tgaaggcgct
    17761 gaaagggcgc tatgagatgg ttaacattaa gcttgataaa accggtggtc tgacggaagc
    17821 gctggcgctg gcgactgaag cgcgtgcaca aggttttcgt ctgatgctgg gctgcatgtt
    17881 gtgtacctct cgtgccatta gcgccgcttt accgctggtg ccgcaggtca gtttcgccga
    17941 tcttgacgga ccgacctggc tggcggtaga tgtggaaccg gcgcttcagt tcacgacggg
    18001 cgaattgcat ctttaggatg ccagcgcagc aagtttgcca tcgcaaagag atatttttcg
    18061 ctggcttcgt cggaggagat gggtggaaac tctgcggtga tgcaatgcaa gttgagatct
    18121 gcacaccaac tgccgaaaga gccaggcgtt tcataaccga cgctggttac cagaggcaat
    18181 tcaaacgcct gggccagcca ttcacctaat tcgctgtgtc tgggatcttc aatacaggcc
    18241 agcggatcgt ggaatgagac cacccaggca ggttgaatgc ggtgtataag ctggcacagc
    18301 gcctgggttt caggttcgga gccaggtttg tcacctgtca gtaatacaac atcacgttct
    18361 tcagcggcgc tgttccagcg ataaactgtt tcaccttcct tccagttcgc tgccggaaag
    18421 tttcggttta aatccacacc attagcattg gcccgtaacc ctaactggca gccgtcagga
    18481 ttcacacaca gcaccacatg atggcggcgc aaagaaggtg tcaaagtccg cagagcacag
    18541 gagagcgtga cgatcgaaga gttttcatca ccgtgagtgc cagccagaat caaaccactt
    18601 tcgtgactgg ctgcaggggc cggaaaccag attaacggcg cgcccaataa tgaacgtccg
    18661 taatgttctg ttccgggcgg aaatgcgccg cgttcggcgc gtgggcgagt tacggtcatg
    18721 atcgtctctg gttcaggaag tattacaggc agtgttgtgc aaattttgcg gattatcaaa
    18781 tccttttctt caacgctatg tcatcgatct tgtatcactt ttttgccgga agttgtttgt
    18841 attttcattg atgtgcagat attagaattg tcgtaatgtt atatacatgg cgtattttat
    18901 tcttactggt tctacacatg ttaatcgcat ctgattgatc agttcacctt aataagttta
    18961 ataaatcgta ctgcacatag tacagtacga tggtagctat ataattgatt tacatgaaga
    19021 agggcttatc accatcagtc ccgggttgat tgatgcctat attctcacca atgtgtttct
    19081 caggtttatc gatgatatct gtgattaatg ctgcgacact cttacgtgat acgatcgtac
    19141 ctttgaatgg ttcattgcgt gaggtaagct cataatcaat aatatcttca tcagtcagcc
    19201 aggcagggcg aagaattgta tattccagtc cagaagcctc aattgcatcc gcagccctgc
    19261 ggaatggttt taggggttca ccaataactg cattattcca ttcgccaaat tttcctggaa
    19321 cttcatcata aataccgagg gaaagaacaa agatcagacg tttaacatca caggctttca
    19381 tcgcagcgat cactgaattt gcctgaatat ctaaatcttc ccccgtaagg ttggcataca
    19441 caacatcctg tccctgcatg gcctgcttca gtgctgcatg attcagtaca tcccccataa
    19501 taatcttgct attagtggga tagggtttgt ggatttttgc tggctgacgg gcaaataaag
    19561 tttgtttaat ggtttgttta tctgccagtt gattaatcac atggcgggct atctgaccgc
    19621 cagcaccaag aatcagtaca tttttcatga aacttttccc agattattgg cagcaatatg
    19681 ccaggccata tcactattac ggatttgaat atgtttcatt acagagtttc ctcaaagaac
    19741 ggagctaata ctgaaattgc ttcagccaca tagactttgc cgtcataaag atccatatgg
    19801 tttgcgcctt caacaatgtg atagcgttta tccttgcttg atgctcgatc gtacaggtcg
    19861 tcactcatcc atttgctccc agcctggctg cccgccacaa tctgtatcgg ctgagtcagg
    19921 tacacttccg ccatatggta agcatcatag gtaataatct ggttaaggct gcgcaaagta
    19981 gcgtaacccg gtgctgttgg atactgcgcg cgaggggtgt ggtaatactc ccaggcctga
    20041 cgcagttctt cgttcggcgc atcggactcc ttcattggtg ccagtggcat agtggcgtat
    20101 tctccgctgc taatatcgct ggttcgggcg tttgaacccg cgtcaacgta tggtagggca
    20161 tcaatagatt tcacattgtt ttcccaacca ttacggaaca tcgaaccaat attgaccgca
    20221 ctaacagtac cgatggcctt gatgcggcga tcctgaattg cagcattggc tgtatatcct
    20281 gcaccggcac aaattcccat cgcaccaatt cgggtattgt cgacataaga aagcgttgtc
    20341 aggtaatcaa tcacggcact gacgtcttca gtacgaatgt atgggttttc taactgacgc
    20401 ggctcgccgc cgctttcacc ttgataagat gcgtcataag caatagtgac aaatcccttt
    20461 tccgccagtt tttcggcata ggttccggcc gtttgctctt taacgccccc acctgggtga
    20521 gataccacaa ttgccggata ctgacgggtt tcatcaaatt ttggagggaa atagatcact
    20581 gcagacaaag agatggttgg attattgctg ttagtgaagc tgactttatt attcatcatt
    20641 ccgttcctca tggagttgtc ggttcgtttt aacggttggt gatatcacta tagatattga
    20701 tcattaagtt gattagaccc aaaatcatga ttagacctat catttaaatg attaatagat
    20761 tgtatggaga tactgaatga agcgtgaaga aatcgctgat ctgatggcgt ttgtcgtcgt
    20821 tgcagaggag cgtagcttca ctcgcgcagc agcccgcctg agcatggcgc agtcagcttt
    20881 aagccagata gtgcgccgta tagaagaacg attgggattg cggcttctga cgcgaaccac
    20941 gcgcagcgtt gtacccactg aagcgggcga gcatcttttg tctgttcttg gccctatgtt
    21001 gcatgacata gattctgccc tgacatccct gagcgatctg cagaaccgcc catccgggac
    21061 aatacgtatt actactgtag aacatgcagc aaaaacgata ttgttaccag ctatgcgcac
    21121 attcctgaaa tcgcatcctg aaattgatat tcagcttacc attgattatg gtttgaccga
    21181 tgtcgtttct gaacgttttg atgcaggcgt ccgtctgggt ggggagatgg ataaagatat
    21241 gatcgccatt cgaatcgggc cagacatacc aatggctatt gttggctcac cggattattt
    21301 ttctcgccga agtgctccaa cgtcagtgtc acaattaata gatcatcagg caattaattt
    21361 gtatcttccc acatcgggta cagcaaatcg ctggagatta atacgcggtg gacgtgaagt
    21421 tcgtgttcgc atggaaggtc agcttttact gaatacgata gacctgatca ttgatgctgc
    21481 aattgatggg catggattgg cgtatctacc ttatgatcag gttgagcggg ctattaaaga
    21541 aaaaaaactg atacgtgttt tggataaatt cacaccagat ttacccggtt atcacctgta
    21601 ctatccacac cgtcgacatg ctggctcggc attctcatta tttatagata ggctgaagta
    21661 taaaggtgct gtttagcact acttgctgat acattaattt aattcttctc ttaacgtatt
    21721 ctcagttcct ttcaacgttt tggttatttt ttattcttcg cacaatggcg acagatgctg
    21781 attatgatac cgaaaacggg tttgaatgtg cgaagcccga acgtagtgtt cggagttcta
    21841 tgtgctttac cgcattttgg agactattat ttacactaaa tctgatttga tatattgata
    21901 cttaaaacat ttgatgcttc ctttgtcact tttttgatgg aagttgtttg catttcttta
    21961 aagcgaaaca aataattacg catcaatttt aatgtcggtt agagggaaac ttatgaagca
    22021 ctctgtttca gtcacgtgtt gtgcgctgtt ggtcagcagc atttctcttt cgtatgctgc
    22081 tgaagttccg agcggcacag tactggcaga gaagcaggag ctggtgcgcc acattaaaga
    22141 tgagccagcg tcgctggatc ccgctaaagc cgtgggcctg ccggagattc aggtcattcg
    22201 cgatctgttt gaaggactgg tgaatcagaa cgagaaaggg gagattatcc ccggcgttgc
    22261 gactcagtgg aaaagtaatg acaaccgtat ctggactttt accctgcgcg ataacgcaaa
    22321 atgggcggat ggcacaccgg taacggcgca agattttgtc tacagctggc aacgtctggt
    22381 ggacccgaaa acgctatcgc catttgcatg gtttgccgca ctggcgggaa tcaacaacgc
    22441 acaggcgatt attgatggta aagccgcgcc tgaccagctt ggcgtcaccg cagttgatgc
    22501 ccatactttg aaaatccagc ttgataaacc attgccgtgg tttgtgaatt taaccgctaa
    22561 ctttgccttc ttcccggtgc aaaaagccaa cgtagaaagc agtaaagagt ggacgaaacc
    22621 cggaaatctg atcggcaatg gcgcttatgt tcttaaagag cgcgtagtca atgaaaaact
    22681 ggtcgtagtg ccgaataccc attattggga taacgccaaa acggtactgc aaaaagtgac
    22741 cttcctgcca attaatcagg aatccgcagc cactaagcgt tatctcgcgg gggatattga
    22801 tatcaccgaa tccttcccga aaaatatgta tcagaagttg ttgaaggata ttccggggca
    22861 ggtttatacg ccgccgcagc tcgggaccta ttattatgcg tttaacacgc aaaaagggcc
    22921 gacggcagat cagcgcgttc gcctggcatt aagtatgacg atagatcgcc gcctgatgac
    22981 cgaaaaagtg ttggggacgg gcgaaaagcc agcgtggcat tttacaccag atgttaccgc
    23041 gggatttacg ccggaacctt cgccgtttga acagatgagt caggaagaac tgaatgcgca
    23101 ggcaaaaact ttgttgagcg cagctggtta tggttcgcaa aaaccgctga agttgacgct
    23161 tttgtataac acttcagaaa accatcaaaa aattgcgatt gctgtagctt cgatgtggaa
    23221 aaagaacctg ggcgtagatg ttaaattgca aaaccaggaa tggaaaacct atatcgatag
    23281 ccgtaatacc ggcaattttg atgttatccg cgcctcgtgg gtaggggatt ataatgaacc
    23341 ctccactttc ctgacattat taacgtcaac gcattccggg aatatttcac gctttaacaa
    23401 tccggcatat gacaaagttc tggcccaggc atcgacggaa aataccgtta aagcgcgtaa
    23461 tgccgattac aacgcggcag aaaaaatcct catggagcaa gcaccgattg caccaattta
    23521 tcaatatacc aatggacgat taatcaagcc gtggctgaaa ggttatccca ttaataaccc
    23581 tgaagatgtg gcgtacagtc ggactatgta tattgtgaag cattaatgtg atgggaactg
    23641 gcgttaccct tgtggataac gccagtgatg tctgatttag cgaccctggt ccggcggtgt
    23701 aatgttatcc atatacagcg tctggctggg gaaggcaaag tccgcgccgt gtgactgtac
    23761 aatatcgata atcttcaaat aaacgtcttg ctgtgcggca agccactcag cccataccgt
    23821 ggttttggta aagcaataaa ccataatatt taatgacgaa tcagcaaact ggttgaaata
    23881 aaccagtaag gtttgtcgct ggtcgatggc cgggtgattt ttcagcatct cacgtacggc
    23941 ttcgacaata acgcccactt ttgccgcatc ctcataacgt aaaccaatgg tcgtggtaat
    24001 gcgccggttg gtcattcgtc caggattttc tacgctgatc gacgaaaaca gcgagttcgg
    24061 tacgtacaat ggacgattat caaaggtctt aattttggta attcgccagc caatttccgc
    24121 tactgtacct tcgatatttc tgtccggtga acggatccag tcgccaatac tgaaaggacg
    24181 gtcgaaatag agcataatcc cggaaaagaa gttactcaga atatctttac cggccatacc
    24241 gacagccaga ccaccaatac caccaaaggt caacaagcca gaaaggctca tgccgaaatg
    24301 ttcgccataa agcagaacaa gcaccacaat aatggtgatt ttgataatgc gcgacatgat
    24361 ccgcgcactg gtgatatcgc gacctttttt aatctgctgt ttttcaaact gattaatcag
    24421 cagaaatagc ttaatcgtca gaataaccgc aatcagggac gtacagataa aatcgataac
    24481 gcctggggtg ataaatttga gtttatagtt ttctataac
//
LOCUS       DAZFJL010000071        24267 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFJL010000071 DAZFJL010000000
VERSION     DAZFJL010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.071"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            3..1016
                     /locus_tag="ACHJ8C_002715"
     CDS             3..1016
                     /locus_tag="ACHJ8C_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HFO5552550.1"
                     /translation="MLNEGDVVSKNSGMCGDVYIFDRGSNTSPQYSCAKIPKLIDGVS
                     DKEIYARFVNELKNQLKYYHHQYVHWVYDFKEVMGVPVALFRYWGSDLRKIIKSSSSS
                     DINKISIMAYLCAGLRHCYSKGLVSHQDLKPENIFIRNMINDFSVPVEQDVYNFPLVG
                     DFGLANASVDSDFFGGARPYMAPEQWERRPLSSKTDVFALGVILFEMMSGGFHPAGIK
                     LSDFWPKPIEGNTKKWTKAEPWKKWASNGGAISADVGVLVDGEILELVNKMLSSNPDN
                     RPDISVMIDDLLQLLRSRDMSSFVQVSHLISHYDSQASQVSLEESWPYLSQRWKMFKT
                     KFG"
     gene            complement(1167..2342)
                     /locus_tag="ACHJ8C_002716"
     CDS             complement(1167..2342)
                     /locus_tag="ACHJ8C_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5552551.1"
                     /translation="MLTDTQCRTAKPKEKLYRLNDFNGLYLEVKPNGKKAWRYRFKLN
                     GKSSMFALGEYPVVKLAEAREKCEQARKQVADGVSPTQARQLDKIRKVNDASNTFELI
                     AKEWLQMKDWAEITKTRRLDMLERVVFPAIGKLPVREITPHHILKILQETAKRGAPTV
                     AAEARRAISSVFELAVATLRADSDPVWPVRKAIPANKTQHKQALNPQQIGKLLSCFDN
                     SRGSYQVNYCMWLMWWTLARPAEATEAEWTEFDLDNALWTIPAARMKARREHVIPLPS
                     QAVNMLRTLQGLTGHRQHLFPGRDNPLGPMTSHSLRQLLKSLGWSGTYSPHATRTTGS
                     TRLNEMGYRPDAIEAQLAHADTNNVRRTYNHATYLDERKVMMQEWADMLDKWVAGFE"
     gene            complement(2557..3648)
                     /gene="ychF"
                     /locus_tag="ACHJ8C_002717"
     CDS             complement(2557..3648)
                     /gene="ychF"
                     /locus_tag="ACHJ8C_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO5552552.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAVRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(3765..4349)
                     /gene="pth"
                     /locus_tag="ACHJ8C_002718"
     CDS             complement(3765..4349)
                     /gene="pth"
                     /locus_tag="ACHJ8C_002718"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO5552553.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            4627..4905
                     /gene="ychH"
                     /locus_tag="ACHJ8C_002719"
     CDS             4627..4905
                     /gene="ychH"
                     /locus_tag="ACHJ8C_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO5552554.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(4960..6639)
                     /gene="dauA"
                     /locus_tag="ACHJ8C_002720"
     CDS             complement(4960..6639)
                     /gene="dauA"
                     /locus_tag="ACHJ8C_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO5552555.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPDSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNLEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(6764..7711)
                     /gene="prs"
                     /locus_tag="ACHJ8C_002721"
     CDS             complement(6764..7711)
                     /gene="prs"
                     /locus_tag="ACHJ8C_002721"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO5552556.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(7862..8713)
                     /gene="ispE"
                     /locus_tag="ACHJ8C_002722"
     CDS             complement(7862..8713)
                     /gene="ispE"
                     /locus_tag="ACHJ8C_002722"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO5552557.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLSTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMNELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     MPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(8713..9336)
                     /gene="lolB"
                     /locus_tag="ACHJ8C_002723"
     CDS             complement(8713..9336)
                     /gene="lolB"
                     /locus_tag="ACHJ8C_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO5552558.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTSDDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            9550..10806
                     /gene="hemA"
                     /locus_tag="ACHJ8C_002724"
     CDS             9550..10806
                     /gene="hemA"
                     /locus_tag="ACHJ8C_002724"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO5552559.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEERDDLQEALIRWLCDYHNLNEDDLRNSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQIFGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQEASEFMA
                     WLRAQSASETIRDYRSQAEQVRDELTAKALAALEQGGDAQTIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            10848..11930
                     /gene="prfA"
                     /locus_tag="ACHJ8C_002725"
     CDS             10848..11930
                     /gene="prfA"
                     /locus_tag="ACHJ8C_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO5552560.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            11930..12763
                     /gene="prmC"
                     /locus_tag="ACHJ8C_002726"
     CDS             11930..12763
                     /gene="prmC"
                     /locus_tag="ACHJ8C_002726"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO5552561.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            12760..13152
                     /gene="sirB2"
                     /locus_tag="ACHJ8C_002727"
     CDS             12760..13152
                     /gene="sirB2"
                     /locus_tag="ACHJ8C_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO5552562.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHIQPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            13156..13965
                     /gene="sirB1"
                     /locus_tag="ACHJ8C_002728"
     CDS             13156..13965
                     /gene="sirB1"
                     /locus_tag="ACHJ8C_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO5552563.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGEWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            14001..14855
                     /gene="kdsA"
                     /locus_tag="ACHJ8C_002729"
     CDS             14001..14855
                     /gene="kdsA"
                     /locus_tag="ACHJ8C_002729"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO5552564.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(15003..15110)
                     /locus_tag="ACHJ8C_002730"
     CDS             complement(15003..15110)
                     /locus_tag="ACHJ8C_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5552565.1"
                     /translation="MTLAQFAMISWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(15516..16616)
                     /gene="chaA"
                     /locus_tag="ACHJ8C_002731"
     CDS             complement(15516..16616)
                     /gene="chaA"
                     /locus_tag="ACHJ8C_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO5552566.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            16886..17116
                     /gene="chaB"
                     /locus_tag="ACHJ8C_002732"
     CDS             16886..17116
                     /gene="chaB"
                     /locus_tag="ACHJ8C_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO5552567.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVARAAVKHEYAKGDDDKWHKKS"
     gene            17274..17969
                     /gene="chaC"
                     /locus_tag="ACHJ8C_002733"
     CDS             17274..17969
                     /gene="chaC"
                     /locus_tag="ACHJ8C_002733"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO5552568.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHT
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNELLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(18013..18366)
                     /gene="ychN"
                     /locus_tag="ACHJ8C_002734"
     CDS             complement(18013..18366)
                     /gene="ychN"
                     /locus_tag="ACHJ8C_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO5552569.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQENNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            18551..19945
                     /gene="ychO"
                     /locus_tag="ACHJ8C_002735"
                     /gene_synonym="iatA"
     CDS             18551..19945
                     /gene="ychO"
                     /locus_tag="ACHJ8C_002735"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO5552570.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSVNFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSVEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(19946..20596)
                     /gene="narL"
                     /locus_tag="ACHJ8C_002736"
     CDS             complement(19946..20596)
                     /gene="narL"
                     /locus_tag="ACHJ8C_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO5552571.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(20589..22385)
                     /gene="narX"
                     /locus_tag="ACHJ8C_002737"
     CDS             complement(20589..22385)
                     /gene="narX"
                     /locus_tag="ACHJ8C_002737"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO5552572.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALIRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANTSGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQSDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            22724..24115
                     /gene="narK"
                     /locus_tag="ACHJ8C_002738"
     CDS             22724..24115
                     /gene="narK"
                     /locus_tag="ACHJ8C_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO5552573.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         5977 a         5933 c         6260 g         6097 t
ORIGIN      
        1 caattttgaa tgaaggtgat gtggtatcta aaaatagtgg tatgtgtggc gatgtttata
       61 tatttgatcg tggttctaat acaagccctc aatattcatg tgcaaagatc cctaaattga
      121 tagatggagt gtctgataag gaaatctatg ctcggtttgt taacgaactt aaaaatcaat
      181 taaagtacta ccaccatcaa tatgtgcact gggtatatga tttcaaagag gtaatgggag
      241 ttccggtagc actctttcga tactggggaa gtgacctcag aaagattatc aagtcctcct
      301 ccagttctga tattaacaaa atatccatta tggcatattt atgtgctggg cttagacact
      361 gctacagcaa aggacttgtt tctcatcaag atttaaaacc agaaaatata tttattcgaa
      421 atatgataaa tgatttcagt gttccagtgg aacaggatgt atataacttt ccgttggtag
      481 gtgattttgg cttggctaat gcatccgtgg attctgattt ttttggtggg gcacgaccct
      541 atatggcccc tgaacaatgg gaaaggaggc cgttatcttc taagactgat gtgtttgctt
      601 tgggggttat tttatttgag atgatgtcag gaggattcca tcctgcaggg atcaaactga
      661 gtgatttttg gcctaaaccg attgagggaa atactaaaaa atggactaag gctgaaccat
      721 ggaagaagtg ggcatcaaat ggaggggcta tctcagcaga tgttggtgtt ctcgttgatg
      781 gagaaatact agaacttgtt aacaaaatgc tttcatcaaa tcctgataat aggcccgaca
      841 ttagcgtaat gattgatgac ttactacaac tcttaaggtc acgagatatg agtagctttg
      901 tacaagtttc gcacttgatt agtcattatg attctcaggc ctcacaagta agtcttgagg
      961 aatcttggcc gtatctatct cagcgttgga agatgtttaa gacaaagttt gggtgaaatt
     1021 tgatagataa gagagctatt gtaagtatcc ctgcaaacga accatcattt ttagaggttc
     1081 tctggcgctc cgaggtccgc tcttggcaca aaagtggacc ctgaggaatg agttctatcc
     1141 tcgtggttca ctaagtgcag caagttctac tcgaaccctg ctacccattt gtccaacata
     1201 tcagcccatt cctgcatcat cactttacgc tcatcaagat aagtcgcatg gttgtatgtg
     1261 cgacgaacat tgttggtgtc agcatgagca agttgggcct caatagcatc aggtcgatag
     1321 cccatctcgt ttaagcgggt acttcctgtg gttctggttg catgtgggct ataagtaccg
     1381 ctccagccga ggcttttaag tagttgacgc agagagtgtg atgtcattgg acctagcgga
     1441 ttatccctgc ctggaaaaag gtgctgtcga tgcccggtta atccctgtag cgttctgagc
     1501 atgttgacgg cttgggaggg gaggggaata acatgttctc tgcgggcttt catccgcgct
     1561 gccggaatgg tccacagggc gttatcaaga tcgaactctg tccattcagc ttcggtggct
     1621 tctgcaggac gcgctaatgt ccaccacatg agccacatgc aatagttaac ctggtatgag
     1681 ccacgactgt tatcaaaaca gcttaatagc ttcccgattt gttgtgggtt taatgcctgt
     1741 ttgtgctgtg tcttattcgc cggaatcgcc ttacgcactg gccagacagg atcgctatct
     1801 gctctgagcg tcgccaccgc cagttcaaaa acggatgaaa tggcgcggcg ggcttcagcg
     1861 gcaacggtcg gagcgccacg cttagccgtt tcctgaagaa ttttaagaat gtgatgtgga
     1921 gtgatctctc tgactgggag cttaccgatt gcagggaaaa ccactcgttc gagcatatcc
     1981 agtcgccgcg ttttggtaat ttcggcccag tctttcatct gcaaccactc tttagcgatc
     2041 agctcaaagg tgttggatgc gtcgttgacc ttgcggatct tatctaactg gcgagcctgt
     2101 gtcgggctaa ctccatccgc gacctgcttt cgcgcttgct cgcatttttc gcgagcttcg
     2161 gcaagtttga ccaccgggta ctcacctagc gcaaacatgc tggatttacc gttgagttta
     2221 aaccgatagc gccatgcctt tttgccattg ggtttcactt cgaggtagag accgttgaaa
     2281 tcattgagtc gatagagttt ttctttcggt ttggcagtac ggcattgcgt atcagtgagc
     2341 ataacggggc cttgtttatt tattataccg ttattgtact cacttaaaat gaagatggaa
     2401 gaatgttgta ctcatccatg tactcatttt tgatagcgtt gttacgagat gagttgagac
     2461 attatgaaaa gaaaaatcca cgcaagtgcg tggattttat tgtgttttcc agtcctcatg
     2521 cgatttactg agatcgcatg agacaacaga agcgaattag acgttgaaca ggaagttcat
     2581 cacatcgcca tctttaacga tgtaatcttt gccttctgca cgcattttgc ctgcttcttt
     2641 cgcgccttgt tcacctttgt aagtgatgaa atcttcaaac gagatggttt gtgcacggat
     2701 aaagcctttt tcaaaatcag tatggatttt gcccgctgcc tgcggcgcgg ttgcaccaac
     2761 cggaatggtc catgcacgca cttctttcac cccagcggtg aagtaagttt gcaggttcag
     2821 cagcttataa ccggcacgga tcacacggtt caggcccggc tcttccagcc caagctcctg
     2881 cataaactcg tcacgctctt cgtcgtccag ttcggcaatg tctgcttcaa cagcagcaca
     2941 aaccggaact acaacagaac cttctttcgc tgcgatttca cgcacctggt caagatacgg
     3001 gttgttttca aaaccgtctt cgttgacgtt ggcgatgtac attgttggtt ttagcgtcag
     3061 gaagctcagg taacgaacag ccgctttctc ttcagcgctt aaatccagcg cacgcagcat
     3121 acctgcgttt tccagctggg gcaggcattt ttccagtacc gccagctcgg ctttcgcgtc
     3181 tttatcgcca cctttggctt tcttctgtac gcgatgaatc gcacgttcac aggtgtcgag
     3241 atccgccagc gccagttcgg tgttgataac ttcaatatca tccgccgggt taactttgcc
     3301 ggaaacgtga atgatgttgt cattttcaaa gcagcgaaca acgtgaccga tcgcttcggt
     3361 ttcacggatg ttggtcagga actggttacc cagaccttcg cctttcgatg cgccttttac
     3421 cagaccggcg atatcgacaa attccatggt cgtgggaagt gtacgctgcg gtttcacgat
     3481 ttcagccagt tgatccaggc gagggtcagg cattggtacg acgccagtgt tcggctcgat
     3541 ggtgcagaat ggaaagttgg ccgcttcaat accggctttg gtcagcgcgt tgaacagggt
     3601 agatttcccg acgttgggca aaccgacgat accgcatttg aatcccatga tttaaatcac
     3661 cttaatatct taataatcaa cctgttattg ctaacagatt gcagaaatgg aaataacttt
     3721 gcctattata cacggcactc ggcaaaaatg ccgcagacaa cgacttattg cgctttaaag
     3781 gcgtgcaatc ggttcgttgc tttggtcaag ccatctgtaa accacatttc agtacaacgc
     3841 gccgcttcgt cgatggcttc atcaattaac ttctgttcac taacaggcgg tttgcctaac
     3901 acaaaaccga caactttatt tttatcgccc ggatgaccga ttccgatgcg taaacggtga
     3961 aagttagggt tattacccaa tttactgatg atgtctttca gtccattgtg accaccatga
     4021 ccgccaccca acttaaattt ggcgacgcca ggaggcagat ccagttcgtc gtgggccacc
     4081 agaatttcgt ccggattaat gcggaaaaaa ctggccatcg ccgcaacggc tttgccgctg
     4141 agattcataa atgtagtcgg gactaacagg cggacatctt cgcctccaag ggtgactcgc
     4201 gaagtataac caaagaattt agcctcttcg cgcagcggag cgcgcaatcg ctctgccagt
     4261 aagtcaacga accaggcacc agcattatgt cgcgttgcgg cgtattcagc accggggttc
     4321 gccaggccga caatcaattt aatcgtcacg tttttttgtc ctgagtgtgt acataactgg
     4381 cgcgtagttt actggttgcg gccctgcttg acaaaaaact gcgtatcaaa tgcagataat
     4441 gcaattattg cctgagtgga ctattagaaa gtcaaggtgt tcaggcgttt atttgtaaag
     4501 ttttgttgaa ataagggttg taattgtgat cacacccgca cataacccac agggtgttgt
     4561 ctatacttta cacataagga agagggggat tccctgttac aacccagaaa gttccggagg
     4621 tgacatatga aacgcaaaaa cgcttcgtta ctcggtaacg tgctcatggg gttgggtctg
     4681 gtggtaatgg tggtcggcgt ggggtattca atcctcaacc agttaccaca gtttaatatg
     4741 ccccagtatt tcgcacatgg tgctgtgcta agtattttcg tcggtgcgat tctctggctg
     4801 gcgggtgccc gtgttggcgg gcatgaacag gtgtgcgacc gttactggtg ggttcgccac
     4861 tatgacaaac gttgccgccg tagcgacaat cgccgtcata gctaacaaat aatgcagttt
     4921 gctgaccagt cagtttgcgc tgactggtca atctcgtact tataaatccg ccatcgccgc
     4981 gcgacgattc gggaagaatg cgaggcgtcc cgggatcggt tgaatgcctg cgcgagccat
     5041 agtgcgcagt ggctggaatt ccaggttgca cacgcgcagt tcacatcctt cgggcagacg
     5101 cttcacaaaa cgctggaacg catcaagacc accagcatca agtaccggaa cggcatccca
     5161 cttcagaatc acaatccgtt tgccttcaag acgtgactcc aggtccgtga ataagccttc
     5221 tgcggcagca aaaaacagcg ggccaataac gcgcagaacc aggacatcgt ctggaacgtc
     5281 tacgactacc ggtgccaggc gagtcatacg tgcgatacga cgcataaaca gcagcgatgc
     5341 cagcacgatc cccacgctga tggcaataac catatcgaac agcacggtca gcgacatgca
     5401 cagcagcatg acgatgatgt catctttcgg tgcatgacgc agcaagtcga ccactttgtg
     5461 cgcttcactc atgttccacg ccaccatcaa cagcagggct gccatggcgg aaagcggcag
     5521 ccaggagagc agcggtgcca gtaccagcag ggcaagaata accagaatag agtggatcac
     5581 cgccgagata ggggaagttg ccccggcacg gacgttagcg gcagaacgcg cgatggcagc
     5641 tgtagcggta ataccaccaa agaacggggc gataatattc cccaatccct gtccaaccag
     5701 ttcgctgttc gccttgtgtt ttgtcccggt cataccatcc agcaccacgg cgcagagcag
     5761 agattcgatt gcgccgagca ttgccattga gaatgccgca ggcagcagtg tgcgaataga
     5821 atcccaggtt agcgtgaatt ctgaatcagg cagatcccac ggcagcacca gttgcggcag
     5881 cagttgcgga ataccgttac cctgagaacc atcggccagg acgtagtgga attgcgaacc
     5941 gatggtagca acatgtccgc cgagcaggtt aacaatcccc atcaccgcgc aaccagccag
     6001 caaggccgga aggtggccgg gtaaacgtat gcccagacgc ggccagaaaa ccagaatgcc
     6061 aagcgtcaca atgccaatgg ctgcatcacc cacattaatg gtcggcagcg ccataaataa
     6121 tgcgccgact ttttgtagat aatgttccgg gacatgggcc atttgcagac cgagaaaatc
     6181 tttaatctgc atggtaccga tggtgatccc gatacccgag gtgaaaccta aggtgacgga
     6241 aaccggaata tactcaatca ggcgaccaaa gcgtgccagc cccatcagaa tcaaaaagat
     6301 ccccgacagc aaggtcgcaa ccagcagtcc tgccagtcca aactgttgcg aaacgggata
     6361 gagaattacc acaaatgccg cagtcggacc ggaaacgcta aagcgtgacc cccccgtcag
     6421 agcaatgaca atccccgcaa cagctgcggt atataaaccg tactgggggg ccacaccact
     6481 accaatagcc aacgccatcg ccagcgggat agcaataatc ccgacggtta tcccggcaat
     6541 caggtcacgg gtaaaccgtg cggcagtata tttttctttc cagcaagcgt cgatcagagc
     6601 gcggaaaggc atcacatgtg aggaaaatat tttgttcaca ataatgtttc atccgtgagc
     6661 gcatcatctg tcaactaaat ggcaggtgaa ggaggcatag gtcatacaaa tggatattac
     6721 agacaaaaaa acccgccgca gcgggtcttt gagccgggtt cgattagtgt tcgaacatgg
     6781 cagagatcga ttcttcgttg ctgatacgac gaatcgcttc ggccagcata cctgacaggg
     6841 tcagagtacg cacgttcggc agtgatttga tttcatcgct cagcggaatg gtatcgcaga
     6901 caacgacttc atcaattaca gaattacgca ggttgttcgc cgcgttgcca gagaagatcg
     6961 ggtgagtcgc gtacgcaaat acacgtttag caccacgttc tttcagagct tcagcagctt
     7021 tacacagcgt accgccagtg tcgatcatat catcgaccag tacgcagtca cgacctgcaa
     7081 cgtcaccgat gatatgcatc acctgggaaa cgttcgcacg cggacgacgt ttgtcgatga
     7141 ttgccatatc ggtatcgttc agcagcttag cgatagcgcg ggcacgcaca acgccgccga
     7201 tgtccggaga aaccacaatt gggttatcca gattcagctg cagcatgtct tccagcagga
     7261 tcgggctacc aaatacgtta tcaaccggaa cgtcgaagaa accctgaatc tgttcagcgt
     7321 gcagatccac tgtcagcaca cggtcaacac cgacgctgga gaggaagtct gcaaccactt
     7381 tcgcagtgat tggtacacga gcggaacgga cgcgacggtc ctggcgcgca tagccaaagt
     7441 aggggataac agcggtgata cgacctgcgg aagcacgacg cagggcatca accataacga
     7501 ctaattccat caggttgtcg ttagtagggg cacaagtgga ctggatgatg aaaatatcac
     7561 caccgcgtac attttcatta atttgtacac tgacttcgcc gtcgctaaag cgacctacag
     7621 cggcgtcgcc gagtgaagtg tacaggcggt tggcaatacg ttgtgctagt tccggggtgg
     7681 cgttaccagc aaaaagcttc atatcaggca cgagaagaac ctcaggcatg cgtccattgg
     7741 tggaaagaat ctgccgaaaa ctgtgcgggc caggcatgat tctttccagg cggtgtatta
     7801 acgagcgcga tgcaacgtct ggaacaaggt gacgttgtca ccgaaactca gcttgcccgg
     7861 cttaaagcat ggctctgtgc aatggggaaa gattaacgcc tttcgccaca aagccattga
     7921 gccattccgg ggcttgctct agcacctggc gggcttcaga ctctgtatca aattcagcaa
     7981 agacacaggc ccctgtccca gtcaggcgcg acggggcgta ttctaacagc caggaaagca
     8041 ccgcatcaac ctcgcgaaaa cgttttcttg cgataacctc acaatcattg ctgaattcac
     8101 attttagcaa cgtttctatt gacctttttg gcgtattgcg cgggagttca ggatctttaa
     8161 aaatcaccgg tgttggaata cttacaccag ggtgcgccac cagataccac ttctctggcg
     8221 gatccaccgg cattagtatt tcaccaacgc cttcggcaaa cgcggcatga ccacgaacaa
     8281 agacaggaac atctgcgccc agcgtcagcc ccatttccgc cagctcattc atgcttagcc
     8341 cgcattgcca gagatgattt aacgccacca gaaccgtcgc ggcattggat gaaccaccgc
     8401 cgagaccgcc gcccatcggc aaacgcttgt caatgctgat attcgcaccg cttcccgtcg
     8461 aaagacgccc gctgtctgcc gcagttttca tcaacaacct cgctgcgcga acgatcaggt
     8521 tatcttcatg ttccacgcct tcaacgggcg ttaacagacg aatatcccca tcgtcacgaa
     8581 gctcaatgct gatggtgtcg ccgtaatcaa gaaactgaaa cagcgtttgc agcgtgtggt
     8641 aaccatccgc acgctgaccg gtaatgtata aaaacagatt aagttttgcc ggagagggcc
     8701 actgtgtccg cattatttca ctatccagtt atccattttt aacttgatgc gttgaccacc
     8761 gtcggtgagt tccatattgg ctggcatcgc aggttgagtt tttgtgtcat aaccaccgta
     8821 aacaactttc cagtttttgc cattctggct gtaggtgatt tcactcaggc ggtactggtc
     8881 gtcaagtttg tagtcagtcg catcacccgg taaacctaaa atccactggc gcaagctgtt
     8941 gagcggaatt ggcattccgg tcaatttgcc aatcatctct tcggcgtcat cggaggtata
     9001 acgctgacct ttattgtcga ctaactgcac gttacccggt tgagcattca gctccagttc
     9061 cgtgctgccc aatgggttag tcagcagcag acggtagcga tcctggccag tttgttgcca
     9121 gaagaaacgg gcgtacactt tttgttggtc ggaaatataa gcgaatgcac cgcgagtctg
     9181 atactgatta agattacgca cgtcttgctg gtgctgacgc cattgtggcg aatccgggct
     9241 tttgccagga cctttgggcg tggtaacgga acaggcagtg agcacaagag cagccagcgg
     9301 tagcaggcgg ataagacgaa aatcgggcag gggcataata atgacaagtc cttgagatac
     9361 gttgcagtta taacccttaa tgctagcgtt accgttagcc atcgtctatg ttcacgttgt
     9421 cttaattgcc agaatctaac ggctttcggc aattactcca aaagggggcg ctctctttta
     9481 ttgatcttac gcatcctgta tgatgcaagc agactaaccc tatcaacgtt ggtattattt
     9541 cccgcagaca tgaccctttt agcactcggt atcaaccata aaacggcacc tgtatcgctg
     9601 cgagaacgtg tatcgttttc gccggataag ctcgatcagg cgcttgacag cctgcttgcg
     9661 cagccgatgg tgcagggcgg tgtggtgctg tcgacgtgta accgcacaga actttatctc
     9721 agcgttgaag aacgggatga cctgcaagag gcgctcattc gctggttgtg tgattaccat
     9781 aatctcaacg aagacgatct gcgtaatagc ctctactggc atcaggataa cgatgcggtc
     9841 agtcatttaa tgcgcgttgc cagtgggctt gattctctcg tccttggcga gccgcagatc
     9901 ttcggtcagg tgaaaaaagc gtttgccgat tcgcaaaaag gtcatatgaa ggccagcgaa
     9961 ctggaacgca tgtttcagaa atctttctct gtggcgaaac gcgttcgcac tgaaacagat
    10021 atcggtgcca gtgctgtgtc tgtcgctttt gcggcttgta cgctggcgcg gcagatcttt
    10081 gaatcgctct ctacggtgac ggtactgctg gttggcgcgg gcgaaaccat cgagctggtg
    10141 gcgcgtcatc tgcgcgaaca caaagtacag aagatgatta tcgccaatcg cactcgcgaa
    10201 cgtgcccaaa ttctggcaga tgaggttggc gcggaagtga ttgccctgag cgatatcgac
    10261 gaacgtctgc gcgaagccga tatcatcatc agttccaccg ccagcccgtt gccgattatc
    10321 ggtaaaggca tggtggagcg cgcattaaaa agccgtcgca atcaaccaat gctgctggtg
    10381 gatattgccg ttccgcgcga tgttgagccg gaagttggta aactggcgaa tgcttatctt
    10441 tatagcgttg acgatctgca aagcattatt tcgcacaacc tggcgcaacg gaaagctgct
    10501 gctgtggagg cggaaactat cgtcgcccag gaggccagtg aatttatggc atggcttcga
    10561 gcacaaagcg ccagcgaaac tattcgcgat tatcgtagcc aggcagagca ggttcgtgat
    10621 gagttaactg ctaaagcgtt agcggccctt gaacagggcg gcgatgcgca aaccattatg
    10681 caggatctgg catggaaact gactaaccgc ctgatccatg caccaacaaa atcacttcaa
    10741 caggccgccc gtgacgggga taacgaacgc ctgaatattc tgcgcgacag cctcgggctg
    10801 gagtagcagt acatcatttt ctttttttac agggtgcatt tacgcctatg aagccttcta
    10861 tcgttgccaa actggaagcc ctgcatgaac gccatgaaga agttcaggcg ctgctgggtg
    10921 atgcgcaaac catcgctgac caggaacgtt ttcgcgcatt atctcgtgaa tatgcgcagt
    10981 taagtgatgt ttcgcgctgt tttaccgact ggcaacaggt tcaggaagat atcgaaaccg
    11041 cacagatgat gctcgacgat cctgaaatgc gtgagatggc gcaggatgaa ctgcgcgaag
    11101 ctaaagaaaa aagcgagcaa ctggaacagc aattacaggt tctgttacta ccaaaagatc
    11161 ctgatgacga acgtaacgcc ttcctcgaag tccgtgccgg aaccggcggc gacgaagcgg
    11221 cgctgttcgc tggcgatctg ttccgtatgt acagccgtta cgccgaagcc cgccgctggc
    11281 gtgtagaaat catgagcgcc agcgagggtg aacatggtgg ttataaagag atcatcgcca
    11341 aaattagcgg tgatggtgta tatggtcgtt tgaaattcga atctggcggt catcgcgtgc
    11401 agcgtgttcc tgctacggaa tcgcagggtc gtattcatac ttctgcttgt accgttgcgg
    11461 taatgccaga actgcctgat gcagaactgc cggacatcaa cccagcagat ttacgcattg
    11521 atactttccg ctcgtcaggg gcgggtggtc agcacgttaa caccaccgat tcggcaattc
    11581 gtattactca cttgccgacc gggattgttg ttgaatgtca ggacgaacgt tcacaacata
    11641 aaaacaaagc taaagcactt tctgtactcg gtgctcgcat ccacgctgct gaaatggcaa
    11701 aacggcaaca ggccgaagcg tctacccgtc gtaacctgct ggggagtggc gatcgcagcg
    11761 accgtaaccg tacttacaac ttcccgcagg ggcgcgttac cgatcaccgc atcaacctga
    11821 cgctctaccg cctggatgaa gtgatggaag gtaagctgga tatgctgatt gaaccgatta
    11881 tccaggaaca tcaggccgac caactggcgg cgttgtccga gcaggaataa tggaatatca
    11941 acactggtta cgtgaagcaa taagccaact tcaggcgagc gaaagcccgc ggcgtgatgc
    12001 tgaaatcctg ctggagcatg ttaccggcag agggcgtact tttattctcg cctttggtga
    12061 aacgcagctg actgacgaac aatgtcagca acttgatgcg ctactgacgc gtcgtcgcga
    12121 tggtgaaccc attgctcatt taaccggggt gcgagaattc tggtcgttgc cgttatttgt
    12181 ttcgccagcg accttaattc cgcgcccgga tacggagtgt ctggtggagc aggcactggc
    12241 gcggttgcct gaacaacctt gccgtattct cgatctcggg acgggtaccg gggcgattgc
    12301 gctggcgctg gccagcgagc gtccggactg cgaaattacc gctgtagatc gtatgccaga
    12361 tgctgtctct ctggcgcaac gtaatgccca gcatctggct atcaaaaata tccacattct
    12421 gcaaagcgac tggtttagcg cgctagccgg gcagcagttt gcgatgattg tcagcaatcc
    12481 gccgtatatt gacgagcagg acccacatct tcaacaaggc gatgtccgct ttgagccgct
    12541 cactgcgctg gttgcggcag acagtggaat ggcagacatc gtgcatatca tcgaacagtc
    12601 gcgtaacgcg ctggtatccg gcggctttct gcttctggag catggctggc agcagggtga
    12661 agcggtgcga caggcattta tcctcgcggg gtatcatgac gtcgaaacct gccgtgacta
    12721 tggggataac gagcgcgtaa cgctcggccg ctattatcaa tgacaagttt ttctacgttg
    12781 cttagcgttc atctcattag tatcgcgctt tctgttgggc tattgacctt acgtttctgg
    12841 ctacgttatc agaagcatcc acaggcattt gctcgctgga cgcgcattgt gccgccggtt
    12901 gtcgatacgc tgttattgtt aagcggcatt gcgttgatgg ctaaagcgca catccagcca
    12961 ttttccgggc aggcacagtg gctgactgaa aagctgtttg gagttatcat ttatattgtt
    13021 ttgggtttta ttgcactcga ttatcgtcgt atgcacagtc agcaggcgcg cattattgcc
    13081 ttcccgctgg cgttggtggt gctgtacatc atcattaaac tcgccaccac aaaagtaccg
    13141 ttactggggt aagtcatgag atcgttagct gatttcgaat ttaataaagc gccattgtgc
    13201 gaaggcatga tcctggcttg cgaagcaatc cgccgcgatt ttccctcgca agatgtttac
    13261 gacgaactgg agcgtctcgt tagtctggcg aaggaagaaa ttagccagct tctgccatta
    13321 gaagagcagc tagaaaaatt aatcgcgctg ttttacggcg aatgggggtt taaagcctca
    13381 cgcggcgttt atcgtctttc cgatgcatta tggctggacc aggtgttaaa gaatcgacag
    13441 ggcagtgcgg tatcattagg tgcggtttta ttatgggtcg cgaatcgtct cgatttgccg
    13501 ctgctgccgg tgattttccc tacgcagctg atattgcgca ttgaatgtcc ggatggcgaa
    13561 atttggctga ttaatccttt taacggtgaa tcgttaagcg aacatatgct ggatgtgtgg
    13621 ttaaagggaa atatcagccc gtcggcggaa ctgttttatg aagaccttga tgaagctgat
    13681 aacattgagg taatccgcaa attgctggat acactcaaag cttcgttgat ggaagaaaat
    13741 cagatggagc tggcgttacg caccagcgaa gctttattac aattcaaccc ggaagatccc
    13801 tatgaaattc gcgatcgcgg gttgatttat gcgcaactgg attgcgaaca cgttgcgttg
    13861 aacgatttaa gttatttcgt tgaacagtgt ccggaagacc cgatcagcga aatgatccgt
    13921 gcgcaaataa ataacatcgc gcataaacat attgtgctgc attaattaat cgacatttta
    13981 ctcaagatta aggcgatcct atgaaacaaa aagtggttag cattggcgac atcaacgtag
    14041 caaatgacct gccgttcgta ctgtttggcg gtatgaacgt gttggaatct cgcgatctgg
    14101 cgatgcgcat ttgcgagcac tacgtaaccg tgacccagaa actgggtatc ccttacgtgt
    14161 tcaaagcctc ttttgacaaa gccaaccgct cctccatcca ctcttatcgt ggaccgggcc
    14221 tggaagaagg gatgaaaatc ttccaggagt tgaagcagac ttttggcgtg aaaattatca
    14281 ccgacgttca cgaaccaagc caggcacagc ccgttgctga tgttgtagat gtgattcagt
    14341 tgccggcgtt tcttgctcgc cagactgacc tggttgaagc catggcgaaa accggtgcgg
    14401 taattaacgt caagaaaccg cagtttgtca gcccgggaca gatgggtaat atcgttgata
    14461 aattcaaaga aggcggcaac gaaaaagtga ttctttgcga tcgcggtgct aacttcggct
    14521 atgacaacct ggttgttgat atgctgggct tcagcatcat gaagaaagtg tctggtaatt
    14581 cgccggtgat tttcgacgtt acccacgcac tgcaatgtcg cgatccattt ggcgcagctt
    14641 ccggcggtcg ccgtgctcag gtggctgagc tggcaagagc cggtatggcg gtaggtctgg
    14701 cggggctgtt tattgaagcg catccggatc cggaacatgc gaaatgtgat ggtccatccg
    14761 cgctgccgct ggctaaactg gaaccgttcc tcaagcaaat gaaagcgatt gatgatctgg
    14821 tgaaaggttt cgaagaactg gataccagca agtaatcttt ttgtttgaaa aataaagtct
    14881 tagcgttctg cgttaagact ttttttatgg gtgccggata caaaaaaggc cgcaggctgt
    14941 tacccctgcg gccggtttcg ggcgcatatt gccatcacgg cagcctgacg cccgttttca
    15001 ccttacttcc ggttacgcca ccagccgaca atcgctgcgg taataattcc cgccaggatc
    15061 ggtgctgcca ggtcgtgcca ggaaatcatg gcaaactgcg cgagcgtcat atagccgcct
    15121 tgttgtaatg acaacatttt gcggctattc ttgaattgtt gatggttcaa gattagcccc
    15181 cgttctgttg tcaggtttta cctctcaacg tgcgggggtt ttctctttcc agcaaccaat
    15241 gccaccaggg ataaagcccc cgcaacattg cgcctcaccg gataacgccg gcttggtgtg
    15301 gatactacgt cgcaattcat cttcacttca tccctgaaat gtttgcattg aagagtacat
    15361 tccggctttt caacagctgt tgcagtggtt tcgtgcaggt tgtggtacag gctcgcaatt
    15421 ctgattaacg acctgtccag gtagtatgaa gaaatacaat aagcaggaat ttatattatc
    15481 tcccccgatt tccgtcatcg gaggagatat aactgtcagg caaatatcgt catcaaatag
    15541 gcggcaaaca gtgccagatg cgctgcgcca ttgagcacgt tagttcgtcc ggtggagaag
    15601 gagatatggc acagtactaa agaggccacc atcaccacca tttctggcgc accaagtgca
    15661 aactgcaatt cgttacccgt cataaaggca attagcgtga cgacaggtac ggtaagcgaa
    15721 atggttgcca acactgaacc aaagaacaga ttcatcgcgc gctggacctg gttattcaac
    15781 actgctttta acgcacctaa accttccggc gacagaatca acagagccac caggaagcca
    15841 gtaaaggcga caggcgcatt catgctgtcg agcaatgtct ccagcgggct ggcgttcatt
    15901 ttggtgactg caataacggc aatcagatgg acaatcaacc agatggcatg ccacaggctg
    15961 ctatgggcag acggtttacc gtggtgcggg tcgtcatcat cactgtcatc ttcgtgctcg
    16021 tagacaaaca aactttgatg tgttttggtc tggatcagca aaaatacgcc atacattgcc
    16081 gcagaaatta atgctaccag taacgcctga cctgttgaaa aattcgccgc aggcagagcc
    16141 attggaaata ccagtacgat tatcgccagg gggaacagcg caattaaata ctgcttgata
    16201 ccaaacagat tcatatattg ggtggcaaac ttgcgtccgc ccaacaataa tgaaaagcca
    16261 accagcccac cggtaacaat cataatgatt gaatagagcg tatcacgcat tagcgttggc
    16321 gcggcgtcgc cggttgccat taaagctgaa atcaaactga cttcaagaat aaccactgaa
    16381 aggctaagaa taagcgaacc gtaaggttct cccaggcgat gggctaatac gtccgcatga
    16441 cggacaacac taaaggcgct acttaaaata ccaataagcg caagaagatt gatggcaatg
    16501 accactggta gtgtctggct gcttccccac aggaacagca ctaccagcgc cagaaccggg
    16561 aaaataagcg aagtctcctt gtggcgggtt tttaccgcct cttgagcatt tgacattatg
    16621 gttatccctt tgcagatgaa tttatcgaaa atgtaaaaaa tgggtaggaa aaataacaga
    16681 aagtgtctgg atatcggtaa cattttacga atttttaccc cgctgtcgat tttttactca
    16741 tttgggcata aaaataagta atacgtttag acaatgtttg ttttagcact tcttaagaag
    16801 agtctgacat gaaaattctt atgttttggc aagagtagat attgttgacc acacttaatg
    16861 ttcaactttg taaaaggagt caacgatgcc gtataaaacg aaaagcgatc tgccggaaag
    16921 cgtaaagcac gtgcttccgt ctcatgccca ggatatctat aaagaagcgt tcaacagcgc
    16981 atgggatcaa tataaagata aagaagatcg gcgtgatgac gccagtcgcg aagaaacagc
    17041 gcataaagtg gccagggctg ctgtgaagca tgaatatgcc aaaggggatg atgataaatg
    17101 gcataaaaaa tcgtaaaacc ggtagcttag ttaaagctat tcgtgcggtg ttgccttgca
    17161 agtggtccgt ggattgcata ttgtcccgtt agtggtttca aaatgagcag taaaaatgtc
    17221 cggaagacac caaaaagttg tcgcagggaa gtatgcagtg gcggaggtgt aaggtgataa
    17281 cgcgtgattt cttgatgaat gccgattgta aaacggcatt tggtgccatt gaagaatcac
    17341 tcttatggtc agcagaacaa cgggcggctt cgctggcggc gacgctggct tgtcgacctg
    17401 atgagggacc ggtgtggatc ttcggttatg gatcgttgat gtggaatccg gcactggagt
    17461 ttaccgagtc gtgcaccggt acactggttg gatggcatcg cgcgttttgc ctgcgcctga
    17521 ccgccgggcg cgggactgcg caccagccgg gacgaatgct tgcactgaaa gagggcggac
    17581 gcaccacagg cgtcgcctat cgactgccag aagagacgct ggagcaggaa ctaaccctgc
    17641 tgtggaagcg cgagatgatt accggctgtt atctgccaac ctggtgtcag cttgatcttg
    17701 atgatggacg cacagtaaac gccattgtgt ttattatgga cccgcgacat acagaatatg
    17761 aatctgacac tcgcgctcag gtcatagcgc cgttgattgc ggcggcgagc ggtccgctgg
    17821 gaaccaatgc acaatacctg ttttcactgg aacaggagct tatcaaactg ggaatgcagg
    17881 acgatgggct gaatgaattg ctggtatcgg taaaaaaact gctggcggag aattatccgg
    17941 atggtgtgtt acggccggga ttcgcctgag taaactttcc tcatagtggg gcgtcagacg
    18001 ccccctcaaa cattaaaatg tgagcacttt atcggctgac agcgtccatt gcgccagttc
    18061 aacaagagta ccgatttcca ccccatcaat cagaggaagt gtactaatcc cgcgcccgtc
    18121 ggtacaggtt ttgcacaatt tcaccggtac attctgagcg gtaaggatct ccagcatttg
    18181 ctgaatgttg tagccttccc ctggtttttg cccgcgcaac ccggctgtga ccgcatcaga
    18241 catcaggaac agacgcagat ccagattgtt ctcctgctct cgtaacgcaa tggccagccg
    18301 caagctgtta aacaaggatt cgctcccgta aggtgcgcca ttggcaacga tcacgatttt
    18361 ttgcattatt tactcctgta ttcagggaat tagacactca tcttctatct tactgcttct
    18421 gcagcgtctg accaatcggt cacattttta aggattttcc tgaaagcgcg agaaaatacg
    18481 acaaaaggtg ccagtaatcg ttattcttta aggctatggt ttttcatttt taccggaagt
    18541 taccgacgtt ttgagccgtt tcgttcctcg cattattccg ttttatttac tcttgctggc
    18601 ggcaggcggt acagctaacg cacaatctac cttcgagcaa aaagcggcaa atccctttga
    18661 taataacaat gatggtctgc cggatttagg catggcacct gaaaatcatg atggggaaaa
    18721 acactttgct gaaattgtga aagatttcgg cgaaaccagt atgaatgata acgggcttga
    18781 tactggcgag caggcaaaag ctttcgcatt gggaaaggtc cgcgacgcgc ttagtcaaca
    18841 ggttaatcag cacgtagagt cctggctatc accgtgggga aatgccagtg ttgacgtcaa
    18901 agtggataac gaaggacatt tcaccggcag tcgtggaagc tggtttgtgc cgttacaaga
    18961 taatgatcgt tatctcacct ggagccagct tggtcttact cagcaggatg atgggctggt
    19021 gagcaatgtg ggcgttgggc aacgctgggc gcgcggcagc tggctggtgg gttataacac
    19081 tttttatgac aacttgctgg acgaaaatct tcagcgagca ggctttggcg ctgaagcgtg
    19141 gggcgaatat ttgcgactat cggtaaactt ttatcagccg tttgctgcat ggcatgaaca
    19201 gacagccacg caggaacaac ggatggcgcg cgggtacgac ctgacagccc ggatgcgcat
    19261 gccgttctat caacacctca ataccagtgt cagcgtagaa cagtattttg gtgatcgtgt
    19321 cgatttgttt aactctggta cgggttatca caatcccgtc gcgttgagtc tgggattaaa
    19381 ttatacccca gtgccattag tcactgtgac ggcccagcat aaacagggtg aaagtggcga
    19441 gaatcaaaat aacctcggac tgaatcttaa ctaccgcttt ggtgtaccgc tcaaaaaaca
    19501 actttctgcg ggcgaggttg ccgaaagtca gtcgttacgt ggtagtcgct atgacaatcc
    19561 gcagcgaaat aatctgccga ctcttgagta ccgacagcga aaaaccttaa cggtgtttct
    19621 ggcgacacct ccgtgggatc taaaacctgg cgaaacagtg ccgctgaaat tacaaatccg
    19681 cagtcgttac ggtattcggc aactgatttg gcagggcgat acgcagatat taagtttgac
    19741 gccgggcgca caagccaaca gcgtggaggg ctggacgctg atcatgcctg actggcagaa
    19801 cggggaaggg gcgagcaatc actggcgatt gtcggtggtg gtggaagata accaggggca
    19861 gcgtgtctcc tccaatgaga tcacgctaac gcttgtcgaa ccgttcgatg cattgtcaaa
    19921 cgacgaactg cgctgggaac cgtaatcaga aaatgcgctc ctgatgcacc cataccgctg
    19981 cttccacgcg agacttgagc ttcattttct tcagcatgtg cttgacgtgc acttttactg
    20041 tgctttcggt gatatccagg cggcgggcaa tcatcttgtt cggcaaaccc tgggcaatca
    20101 gcttgagtat atcgcgctcg cgtggggtta actggttaac atcgcgttca gtagtggcac
    20161 ggttagcgcg caagctggcg gccagaactg gcgttaatgc ttcgcttaat accatttcgc
    20221 cagcagctgc ctgatgcaat gctttcagca gatcttccgg ttccatatct tttaacagat
    20281 agccatccgc gccgcgtttc agtgcggtga ccacatcttc ttcatggtta gagacgctga
    20341 ataccacaat gcgccctgag agggactttt cgcggagttt atccagcgtt tccagaccat
    20401 tcatgccggg catattgaga tctaacagga tcagatcggg atcaagagat tccgccagtt
    20461 caataccctg ttcgccatta ctcgcttcgc caaccacggt gatatctggt gccatactga
    20521 taagctgttt tacgccagtt cgcagcatcg ggtgatcgtc gatcagcagg atagtagccg
    20581 gttcctgatt actcatgggt atctccttgg acgtctgtga aagttttttc gggaataaag
    20641 gtgacaacca cttcggtgcc acctgattca cgacggcgga cgcggcaatc gcctcgtaaa
    20701 ctttgcgcac ggtcgcgcat tattatcatg ccgtagtgat tgctgcggat ggcattttca
    20761 ggcacgccgc agccgttatc ctggacggtc agtttgacct gattatcgtt ttgcgccacc
    20821 gtcaccacga cctcactcgc ttgcgaatgt ttgagggcgt tacttaatgc ctcacgggca
    20881 atttgcaaca agtggattgc ctgatgcgaa ggcaccagac gaggcggcaa ttgataatcc
    20941 agcttcaccg ggaagccaaa tttggcgctg tactcttcgc aactcgcctc cagcgccgga
    21001 cgtaatccag gctcggtgag ctgcaagcgg aatgtggtga gcaattcacg caactgcgcc
    21061 caggatgcat tcagttcgtt acggatctga cttaacagtt cgcggctgct ttctggcagc
    21121 gcatcgccct gcatctgtaa acaactcacc tgcatcttca tgcaagagag agattgggca
    21181 atagaatcat gcagttcgcg cgcaatggtg gcacgctctt ccatcacgat caactgttgc
    21241 tgacgttcct gatgccgatc cagcgccagc gtggcggtga gttgttcaac cagggtatcc
    21301 accagttgtt gttgatcatg gctaagatga cgcccctgcg gcagggtcgc cagcaaaata
    21361 ccgtactgcg tatgagagtc agccagccgc cacttcaggg ttgtaccgcg atcgccaacg
    21421 ggtaatacgc cgcgcgggca gagctggcag cctttatcat cacaagtcat atctgactgg
    21481 caggtaaact cctgatgatt ctcttcatca tcagtgtcat acacccgcag ttcgatatca
    21541 cgtagcaggg ttaaattctg taatccgttg agtacaggtg acaggcgttc acacagcggg
    21601 gcgcgggaat gcaaacgtcg gttagcctgc cataaaaaag agaggatctg atttttatgc
    21661 tccagcccgg cggttttctc ctgaacccgc tgctcaagta cggcataact ttcggccagt
    21721 tctgcagaca tattgttcaa cgcagttcca agcatcgcca tttcgttgcg cccgctggtg
    21781 tttgcgcgtt gggtaaaatc gcgatggctg acggcactcg ccattgccag cagttgccgc
    21841 cacggttgta gcaatctcgc ccgcaaccag ataatagtga ataccagtaa aagtgccata
    21901 aataccgcca ttacccgatg gaccagcacc actgtctcaa tgcgcatttc cgtggtgcga
    21961 tcaaaaccag ataccagctg atcaagcccg gcaacaaact ggctgacatc cgctgacacc
    22021 gtttcgcggt tttgtgcacg tatcagtgca gggatcagtt cattacgcca gtaatcttgt
    22081 aaaccctgta attgcgccag ttgcccgtct cgttctgctg ctcgagtcaa ctcggcgcta
    22141 aatgctgttt gttccatctc tttaattaag ggcttgtctt tctcgcttaa tggcactgct
    22201 gccaacagac ggtaactttg catgcgcagc gatcccgctt tgttgatcgc atgggcgctg
    22261 ccctgaacgc cttgcaccag ccagccagaa accgccatcc ctgccagtcc aatagcagta
    22321 gaaagcaaca caataagcgc aacctgatta accagggtga gcggagagag acaacgttta
    22381 agcatgtaaa cctcttcctt caggctttaa atgagcaata accttaatga atgtgacgat
    22441 acattctgga atggcagtat tctcggctat tggctgaagt atacccatac ccggaaagag
    22501 ttactcctta tttgccgtgt ggttagtcgc tttacatcgg taagggtagg gattttacag
    22561 taccgtgaaa aatctcataa tttttatgaa atcactgtac tcactatgga taatgataaa
    22621 tatcaatgat agataaagtt atcttatcgt ttgatttata tcaaattgcc tttagctaca
    22681 gacactaagg tggcagacat cgaaacgagt atcagaggtg tctatgagtc actcatccgc
    22741 ccccgaaagg gctactggag ctgtcattac agattggcga ccggaagatc ctgcgttctg
    22801 gcaacaacgc ggtcaacgta ttgccagccg caacctgtgg atttccgttc cctgtctgct
    22861 gctggcgttt tgcgtatgga tgttgttcag cgctgttgcg gtgaacttac cgaaagtcgg
    22921 ttttaatttt acgaccgatc agctatttat gttgactgcg ctgccttcgg tttctggcgc
    22981 gttattacgt gttccatact cctttatggt tcctatcttt ggtggtcgtc gctggacggc
    23041 gttcagcacc ggtattctga ttattccttg cgtctggctg ggttttgccg tgcaggatac
    23101 ctccacgcct tatagcgtct tcatcatcat ttctctgctg tgcggctttg ctggcgcgaa
    23161 cttcgcatcc agtatggcaa acatcagctt cttctttccg aaacagaagc agggtggcgc
    23221 gctgggtctg aatggtggtc tgggcaacat gggcgtcagc gtcatgcagt tggttgctcc
    23281 gctggtggta tcactgtcga ttttcgcagt atttggtagc cagggcgtca aacagccgga
    23341 tgggactgag ctgtatctgg cgaatgcgtc ctggatatgg gtgccgttcc ttgccatctt
    23401 caccattgcg gcgtggtttg gcatgaacga tcttgctacc tcgaaagcct ccatcaagga
    23461 gcagttgccg gtactcaaac ggggtcatct gtggattatg agcctgctgt atctggcaac
    23521 cttcggctcc ttcatcggct tctccgcggg ctttgcgatg ctgtcaaaaa cgcagttccc
    23581 ggatgttcag attctgcaat acgctttctt cgggccgttt attggtgcgc tggcgcgttc
    23641 tgcaggtggt gcattatctg accgtctggg cggaactcgt gtcacgctgg tgaactttat
    23701 cctgatggcg attttcagcg gcctgctgtt cctgacctta ccgactgacg gacagggcgg
    23761 aagcttcatg gcgttcttcg cagtcttcct ggcgctgttc ctgaccgctg ggctgggtag
    23821 tggttccact ttccagatga tttccgtgat cttccgtaaa ctgacaatgg atcgtgtgaa
    23881 agcagaaggg ggttctgacg aacgtgcgat gcgtgaagcg gcaaccgaca cggcggcggc
    23941 gctgggtttc atctctgcga ttggcgcgat tggtggcttc tttatcccga aagcgtttgg
    24001 tagctcgctg gcattaacgg gttcgccagt cggcgcaatg aaagtatttt tgattttcta
    24061 tatcgcctgc gtggtgatta cctgggcggt atatggtcgg cattctaaaa aataaatcgt
    24121 aatatttgat tatcctttgc gcggcatgat gtcgcgcttt ttttatgcgt catttagtta
    24181 caacatacta atgttgtatg gcttatttcg ctggatttca ttaagagtca ttaatatgtt
    24241 acccatgggg aatactcctt aataccc
//
LOCUS       DAZFJL010000072        24152 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFJL010000072 DAZFJL010000000
VERSION     DAZFJL010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.072"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            20..703
                     /locus_tag="ACHJ8C_002739"
     CDS             20..703
                     /locus_tag="ACHJ8C_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO5552574.1"
                     /translation="MNLKKTLLSVLMILQLCLLVGCDYIEKASKVDDLVTQQELQKSK
                     IEALEKQQELDKRKIEHFEKQQTTIINSTKTLAGVVKAVKNKQDEFVFTEFNPAQTQY
                     FILNNGSVGLAGKILSIDAVENGSVIRISLVNLLSVPVSNMGFYATWGGEKPTDINAL
                     AKWQQLLFSTAMNSSLKLLPGQWQDINLTLKGVSPNNLKYLKLAINMANIQFDRLQPA
                     EPPQRKSKK"
     gene            747..5543
                     /locus_tag="ACHJ8C_002740"
     CDS             747..5543
                     /locus_tag="ACHJ8C_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO5552575.1"
                     /translation="MNKIFKVIWNPATGSYSVASETAKSRGKKSGRSKLLISALIVTS
                     AIFTPGAYAGLSLDGGDILESTPLTNYWLAIGQESEATTNNGGTDGVAMAIGLKAKAL
                     GAGSTAFGYDAQASGDRAIAFGQLTEASGNRTIAMGSGATATGDHSLALGGATKTLGM
                     YSVAIGRGATTDSDYALSMGYTAKANGLYSLAMGAESATSNDNAIAIGKKTQAQGVSS
                     IALGNASQASSNSSLAIGDSSEAGAENAIALGKLSNASKINSIALGSNSTASGEGSVA
                     LGENSFAGGINSLALGSQSNANGDNAVALGVGSVAAQDNTVSVGNSTTQRKITNMAAG
                     QIRNGSTEAINGSQLYGLSNSVALRLGGGAGVNEDGSINAPSYRLKSGIYNNVGEALL
                     GIDNDTLHWDKTNKAFSASYLAKNADGSLKERSDQNKIINVAKGTISATSTDVVNGSQ
                     LYDLQQDALLWNGTAFSAAHGSDTTSKITNVAKGTVSATSTDVVNGSQLYDLQQDALL
                     WNGTAFSAAHGTEATSKITNVEDGTISDTSKDAVNGSQLKETKDNVATNTANIADNTT
                     DIATNTANIATNTTDIATNTANIATNTTDIATNTADIATNASSITTLNADVDTLEKDA
                     LLWNGTAFSAKHGTEATSKITNVTAGDLTAGSTDAVNGSQLKTTNDNVSTNTTNITNL
                     TDSVGDLKDDSLLWNKTAGAFSAAHGTEATSKITNLLAGKVSSDSTDAINGSQLYGVA
                     DSFTSYLGGGADISDAGVLSGPTYTIGGTDYTNVGDALAAINTSFSSSLGDALLWDAT
                     AGKFSAKHGINNAPSVITDVANGAVSSTSSDAINGSQLYGVSDYIADALGGNAVVNAD
                     GSITTPTYAIAGGSYNNVGDALEAIDTTLDDALLWDETANGDNGAFSAAHGKDKTASV
                     ITNVANGAISATSSDAINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANTDYN
                     NVGEALDALDENALLWDATANNGAGAYSASHDGKASIITNVADGNIAEDSTDAINGSQ
                     LNATNMMIQQNSEIINQIAGDTSKNYIGDNGAGINYVRTNDSGLAFNDASAAGIGSTA
                     VGYNSVAEGHSSVAIGQGSYSGVETSIALGSESVSSRVIVKGSRNTSVSEEGVVIGYD
                     TTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYYHANS
                     TAEDSLAVGEDSLAMGAKTIVNGNAGIGIGLNTLVLADAINGIAIGSNARANHADSIA
                     MGNGSQTTRGAQTNYTAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQ
                     LKVTDAQVSQNTQSITNLNTQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGADAN
                     AQGKDSVAIGSGSIAAADNSVALGTGSVADEENTISVGSSTNQRRITNVAAGVNATDA
                     VNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDAVNYAQ
                     LKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGG
                     GTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            5955..7610
                     /gene="lldP"
                     /locus_tag="ACHJ8C_002741"
     CDS             5955..7610
                     /gene="lldP"
                     /locus_tag="ACHJ8C_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO5552576.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7610..8386
                     /gene="lldR"
                     /locus_tag="ACHJ8C_002742"
     CDS             7610..8386
                     /gene="lldR"
                     /locus_tag="ACHJ8C_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO5552577.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            8383..9573
                     /gene="lldD"
                     /locus_tag="ACHJ8C_002743"
     CDS             8383..9573
                     /gene="lldD"
                     /locus_tag="ACHJ8C_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO5552578.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            9621..10094
                     /gene="trmL"
                     /locus_tag="ACHJ8C_002744"
     CDS             9621..10094
                     /gene="trmL"
                     /locus_tag="ACHJ8C_002744"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO5552579.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPTEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(10147..10968)
                     /gene="cysE"
                     /locus_tag="ACHJ8C_002745"
     CDS             complement(10147..10968)
                     /gene="cysE"
                     /locus_tag="ACHJ8C_002745"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO5552580.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11048..12067)
                     /gene="gpsA"
                     /locus_tag="ACHJ8C_002746"
     CDS             complement(11048..12067)
                     /gene="gpsA"
                     /locus_tag="ACHJ8C_002746"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO5552581.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12067..12534)
                     /gene="secB"
                     /locus_tag="ACHJ8C_002747"
     CDS             complement(12067..12534)
                     /gene="secB"
                     /locus_tag="ACHJ8C_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO5552582.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12597..12848)
                     /gene="grxC"
                     /locus_tag="ACHJ8C_002748"
     CDS             complement(12597..12848)
                     /gene="grxC"
                     /locus_tag="ACHJ8C_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO5552583.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12989..13420)
                     /locus_tag="ACHJ8C_002749"
     CDS             complement(12989..13420)
                     /locus_tag="ACHJ8C_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO5552584.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13665..15209
                     /gene="gpmM"
                     /locus_tag="ACHJ8C_002750"
                     /gene_synonym="pgmI"
     CDS             13665..15209
                     /gene="gpmM"
                     /locus_tag="ACHJ8C_002750"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO5552585.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRTFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15243..16502
                     /gene="envC"
                     /locus_tag="ACHJ8C_002751"
     CDS             15243..16502
                     /gene="envC"
                     /locus_tag="ACHJ8C_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO5552586.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAYADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALSERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16506..17465
                     /locus_tag="ACHJ8C_002752"
     CDS             16506..17465
                     /locus_tag="ACHJ8C_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO5552587.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPHNPFRGVKLCKPKKPLEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(17452..18486)
                     /gene="waaH"
                     /locus_tag="ACHJ8C_002753"
     CDS             complement(17452..18486)
                     /gene="waaH"
                     /locus_tag="ACHJ8C_002753"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO5552588.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLIAQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNAQRVRYTEQSLYKYYLH
                     NTSVSRLNRQGNKNLNYQRHYIKITRLLEKLNRDYADRIVIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMYKRLISNVRSIKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(18725..19750)
                     /gene="tdh"
                     /locus_tag="ACHJ8C_002754"
     CDS             complement(18725..19750)
                     /gene="tdh"
                     /locus_tag="ACHJ8C_002754"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO5552589.1"
                     /translation="MKALSKLKAEEGIWMTDVPEPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITHAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(19764..20960)
                     /gene="kbl"
                     /locus_tag="ACHJ8C_002755"
     CDS             complement(19764..20960)
                     /gene="kbl"
                     /locus_tag="ACHJ8C_002755"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO5552590.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            21174..22106
                     /gene="rfaD"
                     /locus_tag="ACHJ8C_002756"
     CDS             21174..22106
                     /gene="rfaD"
                     /locus_tag="ACHJ8C_002756"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO5552591.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            22116..23162
                     /gene="rfaF"
                     /locus_tag="ACHJ8C_002757"
     CDS             22116..23162
                     /gene="rfaF"
                     /locus_tag="ACHJ8C_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO5552592.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            23166..24125
                     /gene="rfaC"
                     /locus_tag="ACHJ8C_002758"
     CDS             23166..24125
                     /gene="rfaC"
                     /locus_tag="ACHJ8C_002758"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO5552593.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAKNYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPRENLINLNSQAVLEKLSSL"
BASE COUNT         6098 a         6289 c         6247 g         5518 t
ORIGIN      
        1 atgaaaagga attcatccta tgaacctaaa gaaaacactg ttaagcgtgt taatgatatt
       61 gcaactttgc ttattggtag ggtgtgacta tattgaaaaa gcgagtaagg tcgacgacct
      121 cgttacacag caagagttgc aaaaaagcaa aattgaggcg cttgaaaaac aacaagaact
      181 cgacaagcgc aagatagaac actttgaaaa acaacaaact accatcataa acagtaccaa
      241 aacgctcgct ggtgtggtga aggcagttaa aaacaaacag gacgaatttg tctttacaga
      301 atttaacccg gcacaaaccc aatactttat tttaaataac ggctctgttg gtttggcagg
      361 gaaaatactg tctattgacg cagtagaaaa cggcagtgtt attcgtattt cactggttaa
      421 cttattaagt gttcctgtat caaatatggg tttctacgca acatgggggg gagaaaaacc
      481 caccgacatc aacgcattag caaaatggca gcaattgcta tttagtaccg caatgaactc
      541 ctccctgaaa ttattaccag gtcaatggca agacattaat ttgacgctaa aaggtgtctc
      601 gcccaacaac ctcaaatatc tgaaattagc catcaacatg gcaaatattc agttcgaccg
      661 tcttcaacct gctgaacctc cacagcgaaa aagcaaaaaa taaatgcagc ttctgcgctt
      721 atatcaagga atagagagca ctaataatga acaaaatttt taaagttatc tggaacccgg
      781 cgacaggcag ttacagcgtc gccagcgaaa cggcgaagag ccgtggtaaa aaatcagggc
      841 gcagtaaact actaatttct gcattaattg tcaccagtgc aatttttact ccaggagcat
      901 acgcaggact ctctctcgac ggcggtgaca ttctcgaatc gactccattg accaattact
      961 ggcttgctat tggtcaagag tctgaagcaa ccaccaataa tgggggaact gacggagttg
     1021 ctatggcaat aggattgaaa gccaaagcac tcggggcggg aagtacagca tttggctatg
     1081 atgcacaagc aagcggagat cgagcaatag cgttcggaca attaacagaa gcatcaggca
     1141 atagaacaat agcaatgggt agtggagcaa ctgccactgg tgaccattct ctggcgctag
     1201 ggggggcaac taagactcta ggtatgtatt ccgtggcaat aggccggggt gctactacag
     1261 atagcgacta cgcgctttct atggggtata cggcgaaggc gaatggacta tacagcctgg
     1321 caatgggtgc agaaagtgct acttcgaacg ataacgccat cgcgattggg aaaaaaacgc
     1381 aagcccaggg agtgagttct atagccctgg ggaatgcaag ccaggcatcg agcaactcca
     1441 gccttgcaat aggtgactca agtgaggccg gtgctgaaaa tgctattgca ctgggaaaac
     1501 tcagtaacgc aagtaaaatc aattcaatcg ccctgggtag taacagcact gcatctggtg
     1561 agggctccgt tgcgctggga gagaatagtt ttgctggagg cataaacagt cttgctttgg
     1621 gtagtcaatc caatgcaaac ggtgataacg cagttgccct cggtgtagga tctgtcgcag
     1681 cgcaagacaa taccgtttcg gtagggaaca gcacaacaca acgcaaaatt accaatatgg
     1741 ctgccggtca aataagaaat ggcagtactg aggccattaa cggttcacaa ctttatggac
     1801 taagtaactc agtcgcactc cgactcggag gtggtgctgg cgtaaatgaa gatggaagta
     1861 taaatgcgcc aagctacagg ctgaaaagcg gcatctacaa taatgtggga gaggcgcttt
     1921 tgggaatcga caatgacact ctgcactggg acaaaaccaa taaagcattt agtgccagct
     1981 atctcgccaa aaacgctgat ggctccctaa aggagcgctc agaccaaaac aaaatcatta
     2041 atgttgcaaa gggtactatt tccgccacca gcaccgatgt agtaaacggc tctcaattgt
     2101 acgacctgca gcaggatgcc ctgttgtgga acggcactgc attcagcgcc gcacacggta
     2161 gtgataccac cagcaaaatc actaatgttg ctaaaggtac ggtttctgca accagcaccg
     2221 atgtagtaaa cggctctcaa ttgtacgacc tgcagcagga tgccctgttg tggaacggca
     2281 cagcattcag tgccgcacac ggcaccgaag ccaccagcaa aatcactaat gttgaagatg
     2341 gtactatttc agacaccagt aaagatgcgg ttaacggttc ccagttgaaa gaaaccaaag
     2401 acaatgtggc caccaacaca gccaatatcg ccgataacac tacggatatt gccaccaaca
     2461 ctgccaatat cgccactaac accacggata ttgcaaccaa cactgccaat atcgccacta
     2521 acactacgga tattgcgact aataccgccg atatagctac aaacgctagc agtatcacta
     2581 ctctgaacgc ggacgttgat accctggaaa aagatgccct gctctggaat ggcacagcat
     2641 tcagcgccaa acacggcacc gaagctacca gcaagatcac caatgttacc gctggcgacc
     2701 tgactgctgg cagcactgac gccgttaatg gctcccagct caaaaccacc aacgataacg
     2761 tgtcgaccaa caccaccaac atcaccaacc tgacggattc cgttggcgac cttaaggacg
     2821 attccctgct gtggaacaaa acggctgggg cattcagcgc cgcgcacggc accgaagcca
     2881 ccagcaagat caccaactta ctggctggca aggtatcttc tgacagcacc gatgccatta
     2941 acggctcaca actttatggc gtagcggatt catttacgtc atatcttggt ggtggtgctg
     3001 atatcagcga tgcaggcgta ttaagcgggc caacctacac tattggtggt actgactaca
     3061 ctaacgtcgg tgatgctctg gcagccatta acacatcatt tagctcatca ctcggcgacg
     3121 ccctactttg ggatgcaacc gcaggcaaat tcagcgccaa acacggcatt aataatgctc
     3181 ccagtgtaat cactgatgtt gcaaacggtg cagtctcgtc caccagcagc gacgccatta
     3241 acggttcaca actttatggc gttagtgact acattgccga tgctttgggc gggaacgctg
     3301 tggtgaatgc agacggcagt atcactacac cgacttatgc catagcgggt ggaagttaca
     3361 acaacgtcgg tgacgcgctg gaagcgatcg ataccacgct ggatgatgct ctgctgtggg
     3421 atgagacagc caatggcgat aatggtgcat ttagcgccgc tcacgggaaa gataaaactg
     3481 ccagtgtaat tactaacgtc gcaaatggtg caatctctgc caccagcagc gacgcgatta
     3541 acggctcaca actctatacc accaataagt acatcgctga tgcgctgggt ggtgatgcag
     3601 aagtcaacgc tgacggaact atcactgcac cgacttacac cattgcaaat accgattaca
     3661 acaacgtcgg tgaagctctg gatgcgcttg atgagaacgc gctgctgtgg gatgcgaccg
     3721 ccaataacgg cgcaggtgct tacagcgcca gtcatgacgg caaggccagc attatcacaa
     3781 acgttgctga tggtaacatt gctgaagata gcaccgacgc tatcaacggt tctcagttga
     3841 atgcaaccaa catgatgatc cagcagaaca gcgaaatcat caatcagatt gccggtgaca
     3901 ccagtaaaaa ctacatcgga gataacggtg caggtattaa ctatgtacgt accaacgaca
     3961 gcggcttagc gttcaacgat gccagcgcag caggtattgg ctctaccgct gtgggttata
     4021 actctgttgc cgaaggccat agtagcgtgg ccatcggtca gggcagctac agcggcgttg
     4081 agacgagtat tgcgctgggt agcgaatccg tctccagccg tgtgattgtt aaaggttctc
     4141 gtaacaccag cgtatcggaa gaaggtgttg tgatcggtta tgacaccacg gatggcgaac
     4201 tgcttggcgc gttgtcgatc ggtgatgatg gtaaatatcg tcaaatcatc aacgtcgcgg
     4261 atggttctga agcccatgac gctgttactg ttcgccagtt gcaaaacgcc attggtgcag
     4321 tagcaaccac accaaccaaa tactatcacg ccaactcaac ggctgaagac tcactggcag
     4381 tcggtgaaga ctcgctggca atgggcgcga aaaccatcgt taatggtaat gcgggtattg
     4441 gtatcggcct gaacacttta gttctggctg atgcgatcaa cggtattgct atcggttcta
     4501 acgcacgcgc aaatcatgcc gacagcattg caatgggtaa tggttctcag actacccgtg
     4561 gtgcgcagac caactacact gcctacaaca tggatgcacc gcagaactct gtgggtgaat
     4621 tctccgtagg tagcgaagac ggtcaacgtc agatcaccaa cgtcgcagca ggttcggcgg
     4681 ataccgatgc ggttaacgtg ggtcagttga aagtaacgga cgcgcaggtt tcccagaata
     4741 cccagagcat tactaacctg aacactcagg tcactaatct ggacactcgc gtcaccaata
     4801 tcgaaaacgg cattggcgat atcgtaacca ccggtagcac taagtacttc aagaccaaca
     4861 ccgatggcgc agacgccaac gcgcagggta aagacagtgt tgcgattggt tctggttcca
     4921 ttgctgccgc tgacaacagc gtcgcactgg gcacgggttc cgtagcagac gaagaaaata
     4981 cgatctctgt gggttcttct accaaccagc gccgtatcac caacgttgct gccggtgtta
     5041 atgccaccga tgcggttaac gtttcgcaac tgaagtcttc tgaagcaggc ggcgttcgct
     5101 acgacaccaa agctgatggc tctatcgact acagcaacat cactctcggt ggcggaaatg
     5161 gcggtacgac tcgcatcagc aacgtttctg ctggcgtgaa caacaacgac gcggtgaact
     5221 atgcgcagct gaagcaaagt gtgcaggaaa cgaagcaata caccgatcag cgcatggttg
     5281 agatggataa caaactgtcc aaaactgaaa gcaagctgag tggtggtatc gcttctgcaa
     5341 tggcgatgac cggtctgccg caggcttaca cgccgggtgc cagcatggcg tctattggtg
     5401 gcggtactta caacggtgaa tcggctgttg ctttaggtgt gtcgatggtg agcgccaatg
     5461 gtcgttgggt ctacaaatta caaggtagta ccaatagcca gggtgaatac tccgccgcgc
     5521 tcggtgccgg tattcagtgg taatcatcca ttaacgaatg ggtaaacgcc acattgcctg
     5581 atgcgctacg cttatcaggc ctacggggtg attcagcact accctgtagg ccggataagg
     5641 cgttcacgcc gcatccggca agataaggcg caccagatca acaatctgag ggcaattctc
     5701 tgatgggaat tgcccttttc tttattacct ctctcccaga attggcccta ccaattcttc
     5761 gcttatctga cctctggttc acaattcccc aattaaaact cacatcaatg ttgctaatgc
     5821 ataacattta gttaactatc cattgtcatt atccctacac aacacaattg gcagtgccac
     5881 ttttacacaa cgtgtgacaa ggagatgagc aacagactca ttacacggcg tgtgtggact
     5941 gcaggagacc tgcaatgaat ctctggcaac aaaactatga tcccgccggg aatatctggc
     6001 tttccagcct gatagcatcg cttcccatcc tgtttttctt ctttgcgctg attaagctca
     6061 aactgaaagg atacgtcgcc gcctcatgga cggtggcaat cgcccttgcc gtggctttgc
     6121 tgttctataa aatgccggtc gctaacgcgc tggcctcggt ggtttatggc ttcttctacg
     6181 ggttgtggcc catcgcgtgg atcattattg cagcggtgtt cgtctataag atctcggtga
     6241 aaaccgggca gtttgacatc atccgctcgt ctattctttc gataacccct gaccagcgcc
     6301 tgcaaatgct gatcgtcggt ttctgtttcg gcgctttcct tgaaggagcc gcaggctttg
     6361 gcgcaccggt agcaattacc gccgcattgc tggtcggtct gggctttaaa ccactgtacg
     6421 ccgccggact gtgcctgatt gttaacaccg cgccagtggc atttggtgcg atgggtattc
     6481 cgattctggt cgccgggcag gtaacaggca tcgacagctt tgagattggt cagatggtgg
     6541 ggcgtcagct gccgtttatg accattatcg tgctgttctg gatcatggcg attatggacg
     6601 gctggcgcgg tatcaaagaa acctggcctg cggtcgtggt ggcgggtggt tcgtttgcta
     6661 tcgcacagta cctcagttcc aacttcattg ggccggaact gccagacatt atctcttcgc
     6721 tggtatcgct gctctgtctg acactgttcc tcaaacgctg gcaaccagtt cgcgtattcc
     6781 gcttcggtga cttaggggcg tcacaggttg atatgacgct ggctcacaca ggttacacgg
     6841 caggtcaggt gctgcgcgcc tggacaccgt tcctgttcct gactgccacc gtaacgctgt
     6901 ggagtatccc gccgtttaaa gccctgttcg cttcaggcgg tgcgctgtat gagtgggtga
     6961 tcaacattcc ggtgccgtac ctcgataaac tggttgcccg tatgccgcca gtggtcagcg
     7021 aagcgacagc gtatgccgcc gtgtttaagt ttgactggtt ctctgccact ggcaccgcca
     7081 ttctttttgc tgccctgctc tcgattgtct ggctgaagat gaaaccatct gacgctatca
     7141 gcaccttcgg cagcacgctg aaagaactgg ctctgcctat ctactccatc ggtatggtgc
     7201 tggcgttcgc ctttatctcg aactattccg gactatcatc aacgctggcg ctggcactgg
     7261 cgcacaccgg ccatgcattc accttcttct cgccgttcct cggctggctt ggtgtcttcc
     7321 tgaccggatc ggatacctca tctaacgccc tgttcgccgc gctgcaagcc actgcagcac
     7381 aacaaattgg cgtttctgac ctgttgttgg ttgccgccaa caccaccggt ggtgtcaccg
     7441 gcaaaatgat ctctccgcaa tctatcgcta tcgcctgtgc ggcggtaggc ctggtaggca
     7501 aagagtcaga tctgttccgc tttaccgtca aacacagcct gatcttcacc tgtatggtgg
     7561 gcgtgatcac cacgcttcag gcttatgtct taacgtggat gattccttaa tgattgtttt
     7621 acccagacgc ctgtcagacg aggttgccga tcgtgtgcgg gcgctgattg atgaaaaaaa
     7681 cctggaggcg ggcatgaagt tgcccgctga acgtcaactg gcgatgcaac tcggcgtatc
     7741 gcgtaattca ctgcgcgagg cgctggcaaa actggtgagc gaaggcgtgc tgcttagtcg
     7801 acgcggcggc gggacgttta ttcgctggcg tcatgacaca tggtcggagc aaaacatcgt
     7861 ccagccgctg aaaacgctga tggccgatga tccggattac agtttcgata ttctggaagc
     7921 ccgctacgcc attgaagcca gcaccgcatg gcatgcagca atgcgtgcca cgcctggcga
     7981 aaaagaaaag attcagcttt gttttgaagc aacgctcagc gaagacccgg atctcgcctc
     8041 acaagcggac gttcgttttc atctggcgat tgccgaagcc tcacataaca tcgtgctgct
     8101 gcaaaccatg cgcggtttct tcgatgtcct gcaatcctct gtgaagcata gccgccagcg
     8161 gatgtacctg gtacccccgg ttttttcaca gctgaccgaa caacatcagg cggtcattga
     8221 cgccattttt gccggtgatg ctgacggggc gcgtaaagca atgatggcgc accttagctt
     8281 tgttcacacc accatgaaac gattcgatga agatcaggct cgccatgcac gtattacccg
     8341 cctgcctggt gaccataatg agcattcgag ggagaaaaac gcatgattat ttccgcagcc
     8401 agcgattatc gcgccgcagc gcaacgcatt ctgccgccgt tcctgttcca ctatatggat
     8461 gggggggcat attctgaata cacgctgcgc cgcaacgtgg aagatttgtc agaagtggcg
     8521 ctgcgccagc gtattctgaa aaacatgtct gacttaagcc tggaaacgac gctgtttaat
     8581 gagaaattgt cgatgccggt ggcgctaggt ccggtaggtt tgtgtggcat gtatgcgcga
     8641 cgcggcgaag ttcaggctgc caaagcagcg gatgcgcatg gcattccgtt tactctctcg
     8701 acggtttccg tttgcccgat tgaagaagtc gctccggcta tcaaacgtcc gatgtggttc
     8761 cagctttatg tgctgcgcga tcgcggcttt atgcgtaacg cactggagcg agcaaaagcc
     8821 gcgggttgtt cgacgctggt tttcaccgtg gatatgccaa cgccgggagc gcgttatcgt
     8881 gatgcgcatt ctgggatgag cggcccgaac gcggcaatgc gccgctattt gcaggcggtg
     8941 acgcatccgc aatgggcgtg ggatgtgggc ctgaacggtc gtccgcatga tttaggtaat
     9001 atctcggctt acctcggcaa accgaccgga ctggaagatt acatcggctg gctggggaat
     9061 aacttcgatc cgtccatctc atggaaagac cttgagtgga tccgcgattt ctgggatggc
     9121 ccgatggtga tcaaagggat cctcgatccg gaagatgcgc gcgatgcagt acgttttggt
     9181 gctgatggaa ttgtggtttc taaccacggt ggccgccagt tagatggcgt actctcttct
     9241 gctcgtgcgc tgcctgctat tgcggatgcg gtgaaaggtg atatcgccat tctggcggat
     9301 agcggaatac gtaacgggct tgatgtcgtg cgtatgattg cgctcggtgc cgacaccgta
     9361 ctgctgggtc gtgctttcct gtatgcgctg gcaacagcgg gccaggcggg cgtggcaaat
     9421 ctgcttaatc taatcgagaa agagatgaaa gtggcaatga cgctgaccgg cgcgaaatcg
     9481 atcagtgaaa ttacgcaaga ttcgctggtg caggggctgg gtaaagagtt gcctgcggcg
     9541 ctggctccga tggcgaaagg gaatgcggct taagagttag atgaatatct gctatcctgc
     9601 ccccgcacta agggggcagt atgctaaaca tcgtacttta cgaaccagaa attccgccaa
     9661 atactggcaa catcatccgt ctttgcgcta ataccggctt tcgtctgcat atcatcgaac
     9721 cgatgggatt tgcctgggac gataagcgcc tgcgccgcgc ggggctggac tatcacgagt
     9781 ttaccgccgt tacgcgtcat catgactatc gcgcgttcct tgaagcagaa aatccccagc
     9841 gcctttttgc cctcaccacg aaaggtacgc ctgctcacag cgccgtaagc tatcaggatg
     9901 gcgattatct gatgttcggc ccggaaacac gcggcctgcc agcgagcatt cttgatgccc
     9961 tgcccactga acaaaaaatt cgtattccaa tggtgccgga cagccgcagc atgaacctgt
    10021 ccaatgcggt gtcggtcgtg gtgtatgaag cctggcggca gctggggtat ccgggagcgg
    10081 tgttgagaga ttagttactg taggccggat agatgattac atcgcatccg gcacgatcac
    10141 aggacatcag atcccatcac catactcaaa cgtatggtta atgccgttga aatgctgatc
    10201 catatccatt gacggtttat cgctatccgg cttgccgaca atgcgtgccg gaacgccagc
    10261 ggcagtagtg tgcggcggca ccggttgcaa caccacggaa cctgcgccaa tcttcgcgcc
    10321 gcgcccaact tcaatattac cgagaatttt cgcgcccgcg ccaatcatca cgccttcacg
    10381 aattttcggg tgacggtcac caccagattt acccgtaccg ccaagcgtca cagattgcag
    10441 aatcgatacg tcgttttcaa tcaccgccgt ttcaccaacg acgatgcctg tcgcgtggtc
    10501 aagcatgata ccgcgaccaa tttttgctgc cgggtgaata tcaacctgga acgttacaga
    10561 gacctggttt tgcagaaata ttgccagtgc gcggcggccc tgattccaca gccagtgacc
    10621 gatacgatag gcctgcaagg catgaaaacc tttcagatat aacaatggag tcgagtattt
    10681 atccaccgcc gggtcgcggg tacgcactgc ctgaatatca caggccgcag aagcgatcat
    10741 ttccgggtcg gcggcgtagg cttcttccac cacttcacgg atagcaatag ccggcataat
    10801 gggcgatgac agcttgttcg ccagcatgta gctcagtgca ctgccaaggt tttcgtgctt
    10861 gagtagcgtc gcgtggtaaa aactggccag cattggctca cagtccgcca gcgttctggc
    10921 ttcagcttta atattgttcc agacaatttc cagttcttca cacgacattg cttactccac
    10981 acgatgagat aatgaccggc ccgttctgcg cgggccgggt catagtggta acaaaggttc
    11041 cctgggttta gtggctgctg cgctcgtcct tgcgtgcacg acctagtaaa gtcaatgctg
    11101 cctcgcgcgc gttttttccg caatataata cttgataaat ttcctcggtt attggcattt
    11161 caacgccgaa gcgatgcgcc agttcgcgga cttctttcgt attgcggtag ccttccacca
    11221 cctgacctat cttttcctgc gcgctttgta catccatgcc ctgaccgagc atcatgccaa
    11281 aacggcggtt acgcgactgg ttgtcggtac aggtaagcac cagatcaccc agccccgcca
    11341 tgcccataaa ggtggcagga tcagcaccca gcgccgcgcc aagacgcgac atttcagcca
    11401 gtccacgggt gatcagcgcc gtacgcgcat tcgcgccaaa accgataccg tcggacatcc
    11461 ccgcgccaat ggcaatgacg tttttcaccg cgccgccaag ctgcacaccg atgaaatccg
    11521 ggttgctgta aacgcggaaa cttttgccac agtgcagcaa ttgctggagg tcatcggcaa
    11581 aggtctggtc cgtcgaggcc agcgaaatgg ccgtcggtaa acctgccgcc agttctttcg
    11641 caaacgttgg gccagagata accgccagcg gaatttgatc gcctaaggcc tcacgcgcca
    11701 cgtcctgtaa cagacgtccg gtttctgctt ccagcccttt ggtcgcccac accagacgcg
    11761 catcaggacg catcagcggt ttaatctggc gcagcacttc accaaagaca tggctgggta
    11821 cgacgacgag aatattacgg ctggctgcca gcgcagtggc gagatcgctt tcaagatgga
    11881 gcgtatcggg aaaaggcaca tcggggagaa acgcggcgtt acagcggtcg cgttcaagcg
    11941 ttgcgatatg ttcagggtca tggccccaga ggacaacctc gtggccattt cttgccaggg
    12001 taatggcaag agcggtgccg tacgagccgg caccgatcac agtcattgaa gcattacgtt
    12061 ggttcatcag gcatcctgat gttcttcagt accttcgcca gcctgctgct gcaaatagtt
    12121 catgaacagc gcatcgaagt taaccggcgc aaggttcagt tgcgggaatg taccgcggga
    12181 taccatgctg gtgatgcact cacgagcata cgggaacaga atgttcgggc agtatgcgcc
    12241 caggcaatgt gccatctggg tgccttcgat acccgcgatg gagaaaatac cgccctgctg
    12301 aacttcacac aggaacgcgg tttcttcgcc cagggaggcc gttacggtaa cacgcagtac
    12361 cacttcgtaa acgtcatctg ccagttgagt agatgccgta tccagatcaa gtttaacttc
    12421 tggttgccaa tctttctgga aaacgtgcgg cgcgttcggc gcttcgaaag aaatatcctt
    12481 ggtataaata cgttggatct ggaaagtcat ttcggtgttg ttttgttctg acatgtgtag
    12541 aaaaccctta agtgttgtcc ttaaatactg cgcaatgtaa tgccaacgtt cacacgttat
    12601 ttcagcaggg gatccagtcc accacgtgca tccaatgcat acaagtcatc acagccacca
    12661 atgtgctgtg cgtcaataaa aatctgtgga accgtggtgc gaccgctgcg tttgatcatc
    12721 tcttcacgct tagcggcatt gccatcgatc ggcaactcct gaaaactcac gcccttgctg
    12781 ctcagcagtg cttttgcacg atggcaatac gggcaggttt ctttggtata gatttcaaca
    12841 ttggccataa attagctcct gttttttacc ctgaaatttc atgttgcagg gcggcagcaa
    12901 aatggagtgt ccccagaagc ttatatcaat aagtcgctgg gggcctaatt gcagctaacg
    12961 gcctgcatca tgaaagacga caggtaaatt atttgccgcg caccaaaggc aagttttcgc
    13021 cagcccagcc agcgacgcct tctttcagta cgaatacttg cgcaaaacca gctttcgtca
    13081 gcgcgtttgc aggctcctgg cactgcatgc cagaaccatc taccacgata accggtttgt
    13141 ctttgtgctt ctcaagctca ccaacattgt tggctttgat ttcgctcggc aacaggttga
    13201 tagaacctgc gatatggcct ttacggaagt catcacgctg acgtaaatcc acaaccacag
    13261 cgtcttcttt gttgatcaga cgcgtagctt caccacgagt aatcaccttc actttcgagg
    13321 tcaggctctt aaacgtagtc acaagaaccg ccaccagtaa cgcgatccag gcgatactca
    13381 gtatgggatg acggccaaca aattgcataa tttcttgcat ggggggtaac aactcccgac
    13441 gtagtgatta aaaaaaccag gaaaggagta tacctgcgca gtgcggcaaa tacagccagc
    13501 gcgttaactg gaatgcaatt ttgcggggcg ctacgaaaaa aaagcgtaaa tctggcatct
    13561 gcttgccgcg agcggtcgta tattttgatc ttcagaggct attttatcga ttcagctgta
    13621 gtaaaattac gcaaattttg actcttgagt atgaggttgt cgcaatgtcg gtttctaaaa
    13681 aacctatggt actggtgatt ctggatggct atggctatcg cgaagaacag caggataacg
    13741 ccatttttag tgctaaaacc ccggtaatgg atgcactgtg ggccaatcgt ccgcataccc
    13801 tgatcgacgc ttccggtctg gaagtcggtc tgcctgaccg tcagatgggt aactccgaag
    13861 taggccacgt taacctgggt gccggccgca tcgtgtatca ggacctgact cgtctggacg
    13921 ttgaaatcaa agatcgcact ttctttgcta atccggtgct gactggtgcg gtagataaag
    13981 cgaaaaacgc aggcaaagca gtacacatta tgggtctgct ctccgcaggt ggtgtacaca
    14041 gccacgaaga tcacatcatg gcgatggtag aactggcagc tgaacgcggc gcagaaaaaa
    14101 tctacctaca cgcattcctt gacggtcgcg acactccgcc gcgcagtgct gaatcctcgc
    14161 tgaaaaaatt cgaagagaaa tttgccgcgc tgggtaaagg ccgcgtagcg tccatcattg
    14221 gtcgttacta cgcgatggac cgcgataacc gttgggatcg cgttgaaaaa gcttatgacc
    14281 tgctgactct ggcgcaaggt gagttccagg ccgataccgc cgttgccggt ttgcaggctg
    14341 cttatgctcg cgacgaaaac gacgagttcg tgaaagcgac cgttattcgt gcagaaggtc
    14401 agccagatgc ggcaatggaa gacggcgatg cgctgatttt catgaacttc cgtgctgacc
    14461 gcgcgcgtga aatcactcgt gctttcgtga acgctgattt cgatggcttc gcgcgtaaga
    14521 aagtggttaa cgtcgatttc gtgatgctga ccgaatacgc tgccgacatc aaaaccgcag
    14581 tggcttaccc acccgcttcc ctggttaaca ccttcggcga gtggatggcg aaaaacgaca
    14641 aaactcagct gcgtatttcc gaaaccgaaa aatatgccca cgttactttc ttcttcaacg
    14701 gtggcgtaga agagtcgttc aaaggcgaag atcgcattct gatcaactca ccgaaagtgg
    14761 ctacctacga tctgcaaccg gaaatgagct ccgcagagct gaccgaaaaa ctggttgcgg
    14821 caatcaagag cggcaaatac gacaccatta tctgtaacta tccgaacggc gacatggtag
    14881 gtcacaccgg agtgatggaa gcggcggtta aagcggttga agcgctggat cactgcgtgg
    14941 aagaagtcgc gaaagcggtt gagtccgtgg gtggacaact gctgatcacc gctgaccacg
    15001 gtaacgctga acagatgcgc gatccggcaa cgggtcaggc acacacagca cacaccaacc
    15061 tgccagttcc gctgatttac gttggtgata agaacgtgaa agcggttgaa ggcggcaaac
    15121 tttctgacat cgcgccgacc atgttgtcgc tgatgggtat ggaaatcccg caagagatga
    15181 ctggtaagcc gctgttcatc gtggaataat ccctccccat gaggggaaag gcgattaata
    15241 ccatgacacg ggccgtgaaa ccgcgcaggt ttgcaatcag gcccatcatc tacgccagcg
    15301 ttctgagcgc tggcgtattg ttgtgcgcct tttccgccta cgcggatgag cgtgaccaac
    15361 tcaaatccat tcaggctgac atcgccgcga aagagcgcgc ggtacgccaa aagcaacaac
    15421 aacgcgcaag cctgctcgca caattgaaaa agcaggaaga agcgatctct gaagccaccc
    15481 gtaaactgcg cgaaacgcaa aacacgctta atcaactgaa taaacagatt gatgagatga
    15541 acgcgtcgat tgccaaactg gagcagcaaa aagccgccca ggagcgcagc ctcgccgcgc
    15601 aactggatgc cgcgtttcgt cagggtgaac ataccggtat tcagctgatt ctcagcggtg
    15661 aagaaagcca gcgtgggcag cggttacagg cttatttcgg ctatctcaac caggcgcgac
    15721 aagaaaccat tgctcagttg aaacaaacgc gtgaagaagt cgctatgcag cgtgccgaac
    15781 tggaagagaa acaaagcgag caacaaacgc ttttatatga gcagcgcgcc caacaggcga
    15841 agctgaccca ggcgttgagc gagcgtaaga aaacactggc agggctggag tcttccatcc
    15901 agcaaggtca gcaacagttg agcgagctgc gcgccaacga atcccgcctg cgtaacagca
    15961 ttgcccgtgc ggaagctgcg gcgaaagcgc gtgctgaacg tgaagcgcgc gaagcccagg
    16021 cggttcgcga ccgccagaaa gaagcgacgc gcaaaggcac cacctacaag ccgaccgaaa
    16081 gcgaaaaatc gctgatgtcc cgtaccggtg gtctgggcgc accgcgcggt caggcattct
    16141 ggccagttcg cgggccgacg ctgcatcgct atggcgaaca gctacagggt gaattacgct
    16201 ggaaagggat ggtgattggt gcctctgaag gtactgaagt taaagcgatt gccgacggcc
    16261 gggtgattct ggctgactgg ctgcaaggct acggtctggt ggtggtggtt gagcatggta
    16321 aaggcgacat gagtctttac ggctataatc agagcgcact ggtgagcgtt ggttcgcagg
    16381 ttcgcgcggg ccagccaatt gcactggtgg gcagcagtgg cggtcagggt cggccttcac
    16441 tctatttcga aattcgccgc cagggtcagg cggtcaatcc acagccgtgg ttgggaagat
    16501 aagttttgtt tccatttcgt cgtaacgttc ttgcatttgc cgctctgttg gcgctctcct
    16561 cccccgtaat tgctggcaaa cttgccatcg tcattgatga ttttgggtat cgcccgcaca
    16621 acgaaaacca ggtgctggcg atgccttccg ctatctccgt cgctgtatta cccgattcac
    16681 cgcacgccag agagatggcg accaaagcgc ataacagcgg tcacgaagtg ttgattcatc
    16741 taccgatggc accactgagt aaacagccgc tggagaaaaa tacgctacgc ccggagatga
    16801 gcagcgacga aattgagcgc attattcgta gtgcggtcaa taacgtgccc tatgccgtgg
    16861 gaatcaacaa ccacatgggc agcaaaatga cctctaacct gtttggtatg cagaaagtga
    16921 tgcaggcgct ggagcgttac aatctttact tcctcgacag cgtaaccatc ggtaataccc
    16981 aggcgatgcg cgccgcgcaa ggcactggcg tgaaggtgat caaacggaag gtgttcctcg
    17041 acgattcgca aaatgaagcg gacatccgtg tgcaattcaa tcgcgcaatt gacctggcgc
    17101 gtcgcaacgg ttcaaccatt gccattgggc atcctcaccc ttcaacggta cgcgtgttgc
    17161 aacagatggt ttataacctg ccgccagaca ttacactggt gaaagccagc agcttgctga
    17221 atgaaccgca ggttgatact tctacaccgc cgaaaaacgc tgtgcctgat gcaccgcata
    17281 atccgttccg tggcgtgaaa ctgtgtaaac cgaagaaacc gctggaaccg gtttatgcta
    17341 atcgcttctt tgaagtatta agcgaaagca tcagccagag cacgctgatc gtttacttcc
    17401 agcatcagtg gcaaggctgg ggcaagcagc ccgaagcggc aaaacttaac gctagcgcaa
    17461 attaaaggcg ctacgcgtaa tgcgatggtg cgaccgcgtt ttgtcgcgcc attgccataa
    17521 gcggaaagac cacagtaacg cctgataacc cacctttatg ctgcgtacat tggaaatcag
    17581 gcgcttatac ataccggaag taaatatctc agcaatcatc cgttggcggg taataatatc
    17641 tggctcttta cgtaccgcat ggcaaacacg taatgcttcg taagttattt gttgatgaaa
    17701 ttccgggtaa atcactattc tgtctgcgta atcgcgattt aacttttcca acagtcgggt
    17761 aatcttaata tagtggcgtt gataattaag gtttttattt ccctgtctat tcaatcggct
    17821 cactgacgta ttatgcaaat aatatttata taacgattgc tcggtatatc gcacgcgttg
    17881 cgcgttaaac ataaattctg ttgtccagac aatatcttga tgatgtaatc cggcaataaa
    17941 tttaatgtta tttttaacaa tgacatcacg acgataaacc cccatccaga caacgtgagt
    18001 ccaacggcgg gaagaaagcc ccatccgcaa ccagtccggg ccggttaata cgccggttga
    18061 gcgaaggcga tcggtgggga tggactgcca ggtttctccc gtttcacgaa aacaccagtc
    18121 agcattgcac tgcgccacgt cgaggtcgtc ctctaacgcc atggtcatca gcgtttcgta
    18181 catggtggga tagacctcat cgtcagcatc gacaaaagcg acatatttgc ccgtcgccac
    18241 ctcgatccca cgattacgcg ccaccgatgc gccagcattc gcctgatgca acaaacgaac
    18301 gtgcggatag ttttctgcgt aatgctttgc tatttcaaca gaattatccg ttgaaccatc
    18361 gttaataata atgatttcca gagcagtcca ggtttgcgcg attaaagatt ccatgcaagt
    18421 acggaaatca tctcccgcat tatataacgg gataataaca ctgagtttat tggtgctgtt
    18481 catcataaat taccgacgac ctctgaagtt atcctgacgt tttacaggct ttgtattaag
    18541 aaactattaa gcctgtgaga tattattttt acaggtgaga gagtcacttc agggtagaga
    18601 gataatgaga acagctatat aagagatcag cgcaattacg aactctttat tacactgaca
    18661 ggattttaca tatttaacag attatcctca ggaaaaaggg ctggaatacc agcccttgtt
    18721 cgtgttaatc ccagctcaga ataactttcc cggactggcc cgaacgcata gcgtcaaagc
    18781 ccttctggaa atcatcgata gagaaacgat gggtaatgat cggcgagaga tcgaggccag
    18841 actgaatcag cgccgccatc ttgtaccagg tttcaaacat ctcacgaccg taaatacctt
    18901 taatgaacaa gcccttaaag atcactttgg tccagtcgat agacatatca gacggtggaa
    18961 tacccaacat cgcaatgcga ccgccgtggt tcatggtgtc gagcatggta cgaaacgctg
    19021 gcggcgcacc ggacatttcc agaccgacat caaagccttc ggtcatgcct aactccgcca
    19081 tcacgtcatt gagattttct ttggcgacgt taaccgcatg agtgataccc attttacgcg
    19141 ccagctcaag gcggtattca ttaacatccg tgatcaccac gttgcgtgca ccgacgtgtt
    19201 tcgccaccgc cgcagccata atacctatcg gacctgcacc agaaaccagc acatcttcgc
    19261 ccaccagatc aaacgatagc gcggtatgca cggcgttacc gaagggatca aaaatggaag
    19321 ccaggtcgtc ggagatattg tcggggattt tgaaggcatt gaacgccgga atcaccagat
    19381 attctgcgaa gcagcccgga cggttaacac cgacaccgat cgtgttgcgg cacagatggg
    19441 tacgaccgcc acgacagttg cgacagtgac cacaggtgat atggccttca ccagaaacgc
    19501 gatcgccaat cttgaagcct ttcacttcct gaccaatacc gaccacttca ccgacatatt
    19561 catggcccac gaccatcggc accgggatgg ttttttgcga ccactcatcc cagttataga
    19621 tgtgaacgtc agtcccgcag atggctgttt tacggatttt aatcagcaga tcgttatgtc
    19681 cgagttccgg ttcaggaacg tcggtcatcc agatgccctc ttccgctttc agtttggata
    19741 acgctttcat ctcacatcct cactcaggcg attacgccca gttgtttacc aatacgcgtg
    19801 aacgcttcta ccgcacgcgt aatttgctca ggggtatgcg ccgcagacat ctgggtacga
    19861 atacgcgcct gacctttcgg aacgaccgga tagaagaaac cggtgacgta aatcccctct
    19921 ttctgcagct cacgggcaaa tttctgcgcc actactgcat cgccaagcat gaccggaata
    19981 atggcgtgat cggctcccgc cagggtaaag cccgccgccg acatttgctc acggaactga
    20041 cgcgcgttcg cccacagacg gtcacgcagt tcgctgcccg cttcgaccat ctccagcact
    20101 ttgatggaag cggcaacaat ggccggtgcc aacgagttgg agaacaggta cggacgagaa
    20161 cgctggcgca gccactcaac cacttctttg cgcgccgcgg tataaccacc agaagccccg
    20221 cccagcgctt taccgagcgt gccggtgata atatcgaccc ggcccatcac atcgcagtat
    20281 tcatgggaac cgcgaccgtt ttcaccgaca aaaccgaccg cgtgagagtc atccaccatc
    20341 accagcgcat cgtatttatc ggcgagatcg caaacacctt tcaggttggc aatcacgccg
    20401 tccattgaga acacaccatc ggtggcaatc agcacatgac gcgcaccggc ttcacgcgct
    20461 tctttcagac gtgcttccag ctcctgcata tcgttgttgg cataacgata gcgtttagct
    20521 ttgcacagac gcacaccatc aataatagac gcgtggttca gtgcgtcgga gataatggcg
    20581 tcttccgcgc ccaacagcgt ttcgaacagg ccaccgttag catcaaagca ggaagagtag
    20641 agaatcgcat cttccatccc caggaaggcc gccagttttt gttcaagctc tttatggctg
    20701 tcctgagtgc cgcaaataaa acgcaccgaa gccatgccga aaccgtgaga atccattccc
    20761 gcctttgccg ccgcaatcag atccggatga ttcgccagtc cgagatagtt gttggcacaa
    20821 aagttaatga cgtggcttcc atcagccaca gtgatatctg cttgctgcgc agacgtaata
    20881 atgcgctctt ctttaaacaa cccttccgcc cgtgcggttt ccagatcgtt ggttaactgc
    20941 tgataaaatt ctccacgcat tgcgattctc cagacagggc aaattccagc acatattacc
    21001 caaacttata ggtcgcgacg agatgacgcg ttaacacttc tgcaaaattc aggataaata
    21061 acgtccagcc ccctcacttt tggtgaatgg ctgaagccaa cgtcatgtag tgatatgata
    21121 gaagtattcg tgtctgagat tgtctctgac tccataattc gaaggttaca gttatgatca
    21181 tcgttaccgg cggcgcgggc tttatcggaa gcaacatcgt taaagccctg aatgataaag
    21241 gcatcaccga tattctggtg gtggacaacc tgaaagacgg caccaagttt gtgaacctgg
    21301 tggatctgga tatcgcggac tatatggata aggaagactt cctgatccag attatggctg
    21361 gcgaagagtt cggcgatgtc gaagcgattt tccacgaagg cgcgtgctct tccaccaccg
    21421 agtgggacgg caagtatatg atggataaca actatcaata ctccaaagag ctgctgcact
    21481 actgtctgga gcgcgaaatc ccgttcctgt atgcctcttc cgcagccact tacggtggac
    21541 gcacctccga ctttattgaa tcccgcgaat atgaaaaacc gttgaatgtc tacggttact
    21601 caaaattcct gtttgatgaa tatgttcgcc aaatcctgcc tgaagcgaac tcacagattg
    21661 ttggcttccg ttatttcaac gtttatggac cgcgtgaagg ccataaaggc agcatggcga
    21721 gcgtcgcttt ccatctcaac acccagctta ataacggtga atcaccgaag ctgttcgaag
    21781 gtagcgagaa ctttaaacgt gacttcgtct atgtgggcga cgtggcagat gtaaacctgt
    21841 ggttcctgga aaatggcgtt tccggcatct tcaacctcgg caccggtcgt gcggaatcct
    21901 tccaggcagt agcagatgct acgcttgctt atcacaagaa aggccaaatc gaatacattc
    21961 cgttcccgga taaactgaaa ggccgctacc aggcgttcac tcaggcagat ctgacgaatc
    22021 tgcgcgcggc gggttacgat aaaccgttca aaaccgttgc cgaaggcgta acggaataca
    22081 tggcctggct gaatcgtgac gcataagagc tctgcatgaa aatactggtg atcggcccgt
    22141 cttgggttgg cgacatgatg atgtcgcaaa gtctctatcg cacgctccag gcgcgctatc
    22201 cccaggcgat aatcgacgtg atggcaccgg catggtgccg tccattatta tcgcggatgc
    22261 cggaagttaa cgaagcgatc cctatgcctc tcggtcacgg agcgctggaa atcggcgaac
    22321 gccgcaaact gggtcatagt ctgcgtgaaa agcgctacga ccgcgcctac gtcttaccta
    22381 actccttcaa atccgcatta gtgcctttct tcgcgggtat tcctcatcgc accggctggc
    22441 gcggcgagat gcgctacggt ttactcaacg atgtacgcgt gctcgataaa gaagcctggc
    22501 cgctaatggt ggaacgctat gtcgcgctgg cctatgacaa aggcgttatg cgtaccgcac
    22561 aagatctgcc gcaaccattg ttatggccgc agttgcaggt gagcgaaggt gaaaaatcat
    22621 atacctgtaa tcaattttcg ctttcatcag aacgtccgat gattggtttt tgcccgggtg
    22681 cggagtttgg tccggcaaaa cgctggccac actaccacta tgcggagctg gcaaagcagc
    22741 tgattgatga aggttatcag gtggttctgt ttggctcggc gaaagatcat gaggcgggca
    22801 atgagattct tgccgctttg aatacagagc agcaggcatg gtgtcggaat ctggcgggag
    22861 aaacacagct tgatcaagcg gttatcctga ttgcagcctg taaagccatt gttactaacg
    22921 attctggtct gatgcacgtt gcggcggcgc tcaatcgtcc gctggttgcc ctgtatggac
    22981 caagcagccc ggacttcaca ccaccgctat cccataaagc acgcgtgatc cgcctgatta
    23041 ccggctatca caaagtgcgt aaaggtgacg ctgcggaggg ttatcaccag agcttgatcg
    23101 acattactcc ccagcgcgta ctggaagaac tcaacgcgct attgttacaa gaggaagcct
    23161 gacggatgcg ggttctgatc gttaaaacat cgtcgatggg cgatgttctc cacacgctgc
    23221 ccgcactcac tgatgcccag caggcaatcc cagggattaa gtttgactgg gtggtggaag
    23281 aagggttcgc acagattcct tcctggcacg ctgccgttga gcgagttatt cctgtggcaa
    23341 tacgtcgctg gcgtaaagcc tggttctcgg ctcccataaa agctgaacgc aaagcgtttc
    23401 gtgaagcgct acaagcaaag aactatgacg cagttatcga cgcccagggg ctggtaaaaa
    23461 gcgcggcgct ggtgacgcgt ctggcgcatg gcgtaaagca tggaatggac tggcaaaccg
    23521 ctcgcgaacc gttagccagc ctgttttaca atcgtaaaca tcatattgca aaacagcagc
    23581 acgccgtaga acgcacccgc gaactgtttg ccaaaagtct gggctatagc aaaccacaaa
    23641 cccagggcga ttatgctatc gcacagcatt tcctgacgaa cctgcctaca gatgctggcg
    23701 aatatgccgt atttcttcat gcaacaaccc gagatgataa acactggccg gaagaacact
    23761 ggcgagaatt gattggttta ctggctgatt caggaatacg gattaaactt ccgtggggcg
    23821 cgccgcatga ggaagaacgg gcgaaacgac tggcggaagg atttgcttat gttgaagtat
    23881 tgccgaagat gagtctggaa ggcgttgccc gcgtactggc cggggctaaa tttgtagtgt
    23941 cggtggatac ggggttaagc catttaacgg cggcactgga tagacccaat atcacggttt
    24001 atggacctac cgatccggga ttaattggtg ggtatgggaa gaatcagatg gtttgtaggg
    24061 ctccaagaga aaatttaatt aacctcaaca gtcaagcagt tttggaaaag ttatcatcat
    24121 tataaaggta aaacatgcta acatccttta aa
//
LOCUS       DAZFJL010000073        24149 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFJL010000073 DAZFJL010000000
VERSION     DAZFJL010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.073"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..368
                     /locus_tag="ACHJ8C_002759"
     CDS             <1..368
                     /locus_tag="ACHJ8C_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5552594.1"
                     /translation="NKEAAIYSGINVTRLKIKVFMITAVLAGISGMILASRLYSGQPN
                     SALSYELDAIAAAVLGGTSLNGGYGTVVGTVIGALTIGVINNGMNLMNVPYFYQMVVK
                     GLVILVAVYFDVRNKRKRS"
     gene            390..1352
                     /locus_tag="ACHJ8C_002760"
     CDS             390..1352
                     /locus_tag="ACHJ8C_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO5552595.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIREAAQ"
     gene            1349..2395
                     /locus_tag="ACHJ8C_002761"
     CDS             1349..2395
                     /locus_tag="ACHJ8C_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFO5552596.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGVVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDEGETLGLHGRLAATPAEEVGYRTERTDDSIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVMRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSVTPRTP
                     HAAEGLTSHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKTYCFELGIVDGD
                     AEISALKAEIASYR"
     gene            complement(2487..3836)
                     /gene="accC"
                     /locus_tag="ACHJ8C_002762"
     CDS             complement(2487..3836)
                     /gene="accC"
                     /locus_tag="ACHJ8C_002762"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO5552597.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(3847..4317)
                     /gene="accB"
                     /locus_tag="ACHJ8C_002763"
     CDS             complement(3847..4317)
                     /gene="accB"
                     /locus_tag="ACHJ8C_002763"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO5552598.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(4722..5109)
                     /locus_tag="ACHJ8C_002764"
                     /pseudo
     CDS             complement(4722..5109)
                     /locus_tag="ACHJ8C_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240072.1"
                     /note="in Escherichia coli this inner membrane protein was
                     found to anchor the periplasmic catalytic oxidoreductase
                     YedY; sulfite oxidase activity not demonstrated; contains
                     heme; frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
     gene            complement(5295..6269)
                     /gene="yhdH"
                     /locus_tag="ACHJ8C_002765"
     CDS             complement(5295..6269)
                     /gene="yhdH"
                     /locus_tag="ACHJ8C_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO5552599.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRASEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGGWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASHILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            6421..8361
                     /gene="csrD"
                     /locus_tag="ACHJ8C_002766"
     CDS             6421..8361
                     /gene="csrD"
                     /locus_tag="ACHJ8C_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO5552600.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLLSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTND
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLCEIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRRRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            8666..9709
                     /gene="mreB"
                     /locus_tag="ACHJ8C_002767"
     CDS             8666..9709
                     /gene="mreB"
                     /locus_tag="ACHJ8C_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO5552601.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            9775..10878
                     /gene="mreC"
                     /locus_tag="ACHJ8C_002768"
     CDS             9775..10878
                     /gene="mreC"
                     /locus_tag="ACHJ8C_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO5552602.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGITQPTPQQPATGNAVTA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            10878..11366
                     /gene="mreD"
                     /locus_tag="ACHJ8C_002769"
     CDS             10878..11366
                     /gene="mreD"
                     /locus_tag="ACHJ8C_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO5552603.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11375..11968
                     /gene="yhdE"
                     /locus_tag="ACHJ8C_002770"
     CDS             11375..11968
                     /gene="yhdE"
                     /locus_tag="ACHJ8C_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO5552604.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSFHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            11958..13427
                     /gene="rng"
                     /locus_tag="ACHJ8C_002771"
     CDS             11958..13427
                     /gene="rng"
                     /locus_tag="ACHJ8C_002771"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO5552605.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13495..17295
                     /gene="yhdP"
                     /locus_tag="ACHJ8C_002772"
     CDS             13495..17295
                     /gene="yhdP"
                     /locus_tag="ACHJ8C_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO5552606.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGVRPLVAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGFLPAAVNEALSGSVAWDGKVGIELPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTQSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFARLTAEGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            17451..18896
                     /gene="tldD"
                     /locus_tag="ACHJ8C_002773"
     CDS             17451..18896
                     /gene="tldD"
                     /locus_tag="ACHJ8C_002773"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO5552607.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19024..19953)
                     /gene="aaeR"
                     /locus_tag="ACHJ8C_002774"
     CDS             complement(19024..19953)
                     /gene="aaeR"
                     /locus_tag="ACHJ8C_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO5552608.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            20136..20339
                     /gene="aaeX"
                     /locus_tag="ACHJ8C_002775"
     CDS             20136..20339
                     /gene="aaeX"
                     /locus_tag="ACHJ8C_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO5552609.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            20347..21279
                     /gene="aaeA"
                     /locus_tag="ACHJ8C_002776"
     CDS             20347..21279
                     /gene="aaeA"
                     /locus_tag="ACHJ8C_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO5552610.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            21285..23252
                     /gene="aaeB"
                     /locus_tag="ACHJ8C_002777"
     CDS             21285..23252
                     /gene="aaeB"
                     /locus_tag="ACHJ8C_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO5552611.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELISDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLTGMLHKYQHALTDS"
     gene            23344..23616
                     /gene="yhcO"
                     /locus_tag="ACHJ8C_002778"
     CDS             23344..23616
                     /gene="yhcO"
                     /locus_tag="ACHJ8C_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO5552612.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(23671..23934)
                     /gene="yhcN"
                     /locus_tag="ACHJ8C_002779"
     CDS             complement(23671..23934)
                     /gene="yhcN"
                     /locus_tag="ACHJ8C_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO5552613.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         5745 a         5891 c         6558 g         5955 t
ORIGIN      
        1 gtaataaaga agcagcaatt tattcaggga taaatgtcac ccgcttgaaa attaaagtct
       61 ttatgattac agccgtgctg gcaggtatca gcgggatgat ccttgcctca cgcctctatt
      121 ccgggcaacc gaattccgcg ttgagctacg aactggatgc tattgccgcg gctgtactgg
      181 gaggcaccag cctgaatggc ggctatggaa ccgtggtggg gacggtgatc ggcgcattaa
      241 ccatcggcgt tattaataat ggcatgaact taatgaatgt tccttatttt taccagatgg
      301 tggttaaagg attagtcatt cttgtagccg tttattttga cgtaagaaat aaaagaaaac
      361 gcagctaatc atttcaagga attaatcata tgagtaaggt cttcacaatt ggagaaattc
      421 tggttgaaat tatggcgagt aaaatcggcc agccatttga tcaaccaggt atctggaacg
      481 gcccttatcc cagtggtgcg cccgctattt ttatcgacca ggtgacacgt cttggcgttc
      541 cgtgcggcat cattagctgc gtggggaatg atggctttgg tgatattaat atacatcgtc
      601 tggctgctga cggtgtggac atcaggggga tttcagtctt acctctggaa gccaccggta
      661 gtgcctttgt tacttatcat aactctggcg atcgcgattt tatttttaat atcaagaatg
      721 ccgcttgcgg taaattatca gcacagcatg ttgatgaaaa tattctgaag gattgtaccc
      781 attttcatat tatgggttca tccctgtttt cattccatat ggtagacgca gtaaaaaaag
      841 cagtaaccat cgttaaagca aatggtggcg ttatttcttt cgacccgaat attcgtaaag
      901 aaatgcttga tattccagaa atgcgcgatg ccctccactt tgttcttgag ttaacggata
      961 tctatatgcc cagtgagggg gaagtgttgc tcttgtcgcc acactcgacg ccggagcgtg
     1021 caatcgctgg ttttcttgaa gaaggtgtta aagaggtgat tgtgaagcgc ggcaatcaag
     1081 gagccagtta ctactccgcg aatgagcagt ttcatgttga gagctatccg gtggaagaag
     1141 ttgacccgac cggtgcagga gactgctttg gtggcgcgtg gattgcctgc cgccagctag
     1201 ggtttgacgc ccatcgggca ttacaatatg ccaacgcctg cggtgcactg gcggtaaccc
     1261 gacgtggccc aatggaggga acgtctcgcc ttgcggaaat cgaagcgttc atccagcgcc
     1321 atgacatgtc cattcgggaa gcagcacaat gaaaaacgaa atttggtcgt ggattggcga
     1381 tctctcgcag gttgccggaa ttaaacacta tgagttacgt agtggcagag caaagggtac
     1441 agaagcgttt gatgtccgta ctggtgcggg actggcattt acggtggtaa aagatcgcgc
     1501 gctggatatt gcatgggcca gctacaagga cactgcgctc tcttttatca cccccaacgg
     1561 tgttgtcgcc cccgcttttt ttgagtctca gggcaacggg tttttacgta gcttttacgc
     1621 ggggttactg actacctgcg gacttagcta cattggcacg ccctgtgagg atgaaggaga
     1681 aacgctgggg ttacacgggc gtttggctgc gactccagca gaagaggtgg ggtatcgcac
     1741 agaacggacc gacgacagca ttgaatttgt catcaatggc aaagtacggg aaacacgatt
     1801 gttcggtgaa aatctgacgc tggaaaggac cattcgctgc cgttatggcg aaaatgtaat
     1861 gcgcattgaa gacaaggtga ctaatcatgg tttcacccgc cagccgctgc aaattcttta
     1921 tcactttaat tacggatggc ctttattatc gccacaggca gaaatcttgc tgtcggctaa
     1981 aagcgtaacg ccacgaaccc cgcatgcggc ggaagggtta accagtcatc tggagatttg
     2041 taccccacag cccggttttg acgagcaggt ttactatctc acgttgaata gcgacagtca
     2101 ggggatgagt aaagttgcgc tggtgaacgc cgaactgggg tggggaattt acgagaaatt
     2161 cgacaccatg cagctaccca actttatcca gtggaaaaat ttaggcgcgg gtgaatatgt
     2221 tatggggctg gaggtcagca atagttttcc tgacggtcgt gataaagaac gagcgcaggg
     2281 gcgtctgccc tttatagagc caggggaaac aaagacatac tgctttgagc tgggaattgt
     2341 tgatggcgat gctgaaatta gcgcgttgaa agcggaaatt gcaagctacc gttagctaac
     2401 ggcaataaaa acgccgggca gcgttaacag gcccggcgtt gattacgttc tttgctaagc
     2461 gcgatggaaa caccgcgcgt cacaaattat ttttcctgaa gaccgagttt tttctccaga
     2521 tagtggatat tagtgccacc atgctggaag ttctcgtcat tcatgatgcg gatctgcaga
     2581 tcaacgttgg ttttgatacc gtcgatgatc agctcctgca gcgcattctt catgcgagca
     2641 atcgccacgt cacggttttc accgtagcaa atcagcttac cgatcattga gtcatagtac
     2701 ggcggtacgg tgtagcccgc gtagatatga gactcccaac gtacgccaaa accaccaggt
     2761 gcgtggaaac gggtgatttt gcccggactt ggcaggaagg tgttcggatc ttcggcgttg
     2821 atacggcatt ccaccgcatg gccgcgaacg tgaacttctt cttgcttgat cgacagcggt
     2881 tgaccggcag cgatacgcag ctgttctttg atcaggtcaa cgccggtgat catttcagta
     2941 accgggtgtt ctacctgaat acgggtgttc atttcgatga aatagaactc gccgttttcg
     3001 aacaggaact cgaaagtacc tgcaccgcga tagccgatat caacacacgc tttagcgcaa
     3061 cgttcgccga tgtagcgacg cagttccggg gtaatgcccg gagccggtgc ttcttcgacc
     3121 actttctggt ggcggcgctg catggagcag tcacgttccg ccagatagat agcgttgccc
     3181 tgaccgtcag ccagtacctg aatctcgacg tggcgaggat tttccaggta tttctccatg
     3241 taaaccatat cgttgctgaa agctgctttc gcttccgcac gggtcatgga gatggattgt
     3301 gccagttcag cgtcgccgcg cactacgcgc ataccgcgac caccgccgcc gccggaggct
     3361 ttgataatca ccggataacc aatgcgttta gcaatggcgc ggtttttatc catatcgtcg
     3421 cccagcgggc cgtcagaacc cggtacgcaa ggaacgcctg cttttttcat cgccgcgatt
     3481 gcggatactt tgtcgcccat caggcgaatg gtttctgctt tcgggccgat gaagataaag
     3541 ccggagcgtt caacctgctc ggcaaaattg gcgttctcgg agaggaagcc gtaacccgga
     3601 tggattgcta ctgcgccggt gatttcagcg gcgctgatga ttgccgggat gttcagataa
     3661 ctttttactg acggagcagg gccgatacag accgtttcat ctgccagtaa tacgtgtttt
     3721 agatcgcgat ccgcactgga gtgcacagcg acagtcttga tgcccagttc tttacaggca
     3781 cgaagaatac gcaatgcaat ctcgccgcgg ttggcaataa caattttatc cagcatgttc
     3841 gcctcgttac tcgatgacga ccagcggctc gtcaaattct accggttgtc cactttcgac
     3901 cagaattgct ttcacggtac cggatttgtc cgcttcgatc tggttcatca ttttcatggc
     3961 ttcaacgatg cacagggtat cgcccacgtt gactttctga cccacttcga tgaacgcttt
     4021 tgcgtccggg cttggggtgc ggtagaaagt accaaccatc ggggaacgta cgatgtgacc
     4081 actgatttcc gctgctgctg gcgcttccat ggaaggaacg gtcgccggag cggctgcgtt
     4141 agattgagct ggctgctgca tcataggtgc agcgtaagct tgttgcatca cagggaaact
     4201 tgcggcagga gctgcacggc taatgcgtac tgactcttca ccttcagaaa tttccagttc
     4261 ggagatgcct gattcttcaa ccagctcgat cagtttttta atcttacgaa tatccatgag
     4321 tgggttccgt actctttgtt tagtgtgatt gtgacaggcg tttcaccgcc gtctgtaaag
     4381 cgtatgcccc gacagctgta tgcatagcga taaattccag caggccggga cgccggtcta
     4441 ttttgcccca gagtgtcaat gttagacttg acggacattg tgcagcgatc tgctgctatc
     4501 ctccggcaaa aaacaaaata taccttcgtt gcgccaatgt caccttttcc gcagcgcaaa
     4561 aactgcgtca gggaggacga ggtcgcacat tataacgatt tcgtagcaat tggcagctaa
     4621 atactggtct tatcagggaa gataatcaac agctaacatg taaataacct tcaacaccgt
     4681 gtaatttgca acaagccgca caattcacga aattagcgcc atcatcgacg gaacttctta
     4741 taacgtaagg tgaaaagctg taaagccagc cctgcgaaga tggggggctg caatgagata
     4801 atcttcacag accacaaata atgtagggat gccaggatcg cgacaagata gacgacatta
     4861 cgcagcggtt gctggggatt acccaatttt cactgcatcg tccggaaaga tgtgaatgct
     4921 aaagcacgca aaattaaccg actgacaatg ttccgcattc aataggttta agtggttaat
     4981 ttgtaatcaa ataaaatcag attattcccc ccatggtacc agaaatcagt aacagattaa
     5041 gcgtagcgtc gccaaggcaa agcaccataa acctaacagg cgacggaggc gtatcaataa
     5101 tggctgtttg gtggagtatc aatgcgcatc aatttatggt cagtcgaaat catgactttc
     5161 cagtttggat gatgttgtac aacttcatga ccaagatgtt cgacgaaagc tgtccggcta
     5221 accgcttttc ccgttaaccg attgaataat gatttcaaaa tgagttccct ctcttattat
     5281 tccctgctaa atggttagtt aaccttcacc agcgtgcgac cctggatctg gttattaatg
     5341 atggcctcgg caaacttcgg tgcctctgcc agagatatct cttttgccgc ctgggtatag
     5401 aatgattccg gtaaatcggc gaccagtcgc tgccaggctt gtgcgcggcg tgctggtggc
     5461 gtcattactg aatccacccc ttgcaaacgg acattacgca gaataaatgg catgaccgtg
     5521 gttggcagag taaaaccacc cgccagacca caggccgcca cgcagccgcc gtaattcatt
     5581 tgcgccagca cttttgccag cactttgtcg ccaacggtgt caattgcccc agcccagact
     5641 tgtttttcca gaggacggga ttcggcaaac tcatcgcgag gaagaatatg gctggcacct
     5701 aaacttttca gatattcatg ggtactttcg cgaccggaaa cagcaacgac ctgataaccc
     5761 aacttatgca gcagcgccac ggcggtactg ccgacgccac cactggcacc cgtcacgaca
     5821 atctccccat cctgcgggcg aacaccggca tcttccagcg ccatcacaca cagcatggcg
     5881 gtaaaaccgg cagtaccgat aatcattgct ttacgcgcgt ccagcccttg cggcatggca
     5941 accagccagc cacctttcac tcgcgcctgt tccgccagcc caccccagtg gttttcacca
     6001 acgccccagc cagtgagtaa cacctcctga ccggcatgaa aacgcggatc ttcgctggcg
     6061 cgtacagttc cggcaaaatc aatcccagga atcatcggaa aatttcggat gatttttccc
     6121 ttaccggtaa tcgccagcgc atctttgtag ttcaggctcg accagtgaac atcaaccgtg
     6181 acatcgcctt ccggcaggcg actttcgtcc agagtctgta ctgatgcgag agttttgccg
     6241 tcctgctgtt ctaaaagtaa cgcctgcata agtggtcctc atgtgcatga tggattggaa
     6301 aataatttct gaagactata ctcgctaatg gaaaagcaaa gccgatgaag cgcaataaat
     6361 tgcgagataa atctgatttg ctagtatgcc cgcttcctca ctatcggagt taacacaagg
     6421 atgagattaa cgacgaaatt ttcggccttt gtcacgctgc tcaccgggtt aacaattttt
     6481 gtgactttgc tgggctgttc gctaagtttc tacaacgcca ttcagtataa gtttagtcat
     6541 cgtgttcagg cggtggcgac ggcgattgat acccacctac tgtcgaatga cttcagcaca
     6601 ttaaggccac aaattaccga attaatgatg tcggcagata tcgttcgtgt agacctgctc
     6661 catggtgata agcaggttta taccctggcc agaaatggta gttatcgtcc ggttggcacc
     6721 aacgatctgt ttcgtgaact gagcgttccg ttgataaagc atccggggat gtcgctgcgt
     6781 ctggtttatc aggatccgat gggcaactat ttccattctt taatgactac cgcgccgctc
     6841 acgggggcga ttggctttat cattcttatg ctcttcctgg cggtacgctg gttacaacgg
     6901 caacttgccg ggcaagaatt gctggaaacc cgggctactc gtatcttaaa cggtgagcgt
     6961 ggctctaatg tgttgggaac catctatgaa tggccgccca gaaccagcag tgcgctggat
     7021 acgctgcttt gtgaaattca gaacgcacgc gaacaacaca gccgtcttga tacgctgatc
     7081 cgctcttatg ccgcccagga catgaaaacc ggcctcaata accgactctt tttcgataat
     7141 cagttagcaa cgttactgga agatcaagag aaagttggta cccacgggat cgtgatgatg
     7201 attcgtctgc cggatttcaa tatgttgagt gatacctggg ggcacagcca ggttgaagaa
     7261 cagttcttct ctctgacgaa tctgctatcg acatttatga tgcgctaccc tggcgcactg
     7321 ctggcgcgtt accaccgcag tgattttgct gcgctgttac cgcaccggac gttaaaagag
     7381 gcagagagca tcgccagtca gttaatcaaa gccgtcgata ccttgccgaa caataaaatg
     7441 ctcgatcgtg acgatatgat ccacattggt atctgtgcct ggcgtagtgg tcaggatacc
     7501 gagcaggtaa tggaacatgc agagtctgcc acgcgtaatg cgggattgca gggcggcaat
     7561 agctgggcta tttacgatga ctcgttgcct gaaaaaggac gcggtaatgt tcgctggcgt
     7621 acgcttatcg agcaaatgct cagtcgcggc ggcccgcgcc tttatcaaaa accggcggtt
     7681 actcgcgaag gccaggttca tcatcgtgaa ctcatgtgcc gcatcttcga tggtaatgaa
     7741 gaggttagct cggcagagta tatgccgatg gtcttgcagt ttggtttatc ggaagagtat
     7801 gaccgcctgc aaatcagccg tcttattcca ctattgcgtt actggccaga ggaaaatctg
     7861 gcgattcagg ttaccgttga gtcgctgatt cgcccgcgtt ttcagcgttg gctgcgcgat
     7921 acgttaatgc aatgtgaaaa atcgcaacga agacgcataa ttattgaact tgcagaggcc
     7981 gatgttggtc aacatatcag tcgcttacaa cctgttattc gtttagtgaa tgctttaggg
     8041 gtacgggtag ccgtcaacca ggctggtttg acgctggtaa gcaccagttg gatcaaagaa
     8101 cttaatgttg agttactcaa gctccatccg gggctggtca gaaacattga gaagcgaacg
     8161 gagaaccagc tgctggttca aagcctggtg gaagcctgct ccgggaccag cacccaggtt
     8221 tacgccaccg gcgtacgttc gcgaagcgag tggcagaccc tgattcagcg cggtgttaca
     8281 ggtgggcaag gggatttttt cgcgtcctca cagccacttg atactaacgt gaaaaaatat
     8341 tcacaaagat actcggttta acctgccgtt taatccgttt tcacgtagaa taacgcgcgc
     8401 tgcgtctcat gggagtgtgc ttgtctgctc gccagattgt tgcagcacat atgcagatga
     8461 atgaccttac gcggttgcaa acaggcgagg aatgctgctg atgcattaag cctttctgga
     8521 ctcaggcaga gatttgtaac aaaggaaacg aactgcacta attttcaccg tagcagatga
     8581 tttttgcgcc ttgtcgctgc tgcgtgtggt tggtaaagta agcggatttt cttttccgcc
     8641 ccagctttca ggattatccc ttagtatgtt gaaaaaattt cgtggcatgt tttccaatga
     8701 cttgtccatt gacctgggta ctgcgaatac cctcatttat gtaaaaggac aaggcatcgt
     8761 attgaatgag ccttccgtgg tggccattcg tcaggatcgt gccggttcac cgaaaagcgt
     8821 agctgcagta ggtcatgacg cgaagcagat gctgggccgt acgccgggca atattgctgc
     8881 cattcgccca atgaaagacg gcgttatcgc cgacttcttc gtgactgaaa aaatgctcca
     8941 gcacttcatc aaacaagtgc acagcaacag ctttatgcgt ccaagcccgc gcgttctggt
     9001 ttgtgtgccg gttggcgcga cccaggttga acgccgcgca attcgtgaat ccgcgcaggg
     9061 cgctggtgcc cgtgaagtct tcctgattga agaaccgatg gctgccgcaa ttggtgctgg
     9121 cctgccggtt tctgaagcga ccggttctat ggtggttgat atcggtggtg gtaccactga
     9181 agttgctgtt atctccttga acggcgtggt ttactcctct tccgtgcgca ttggtggtga
     9241 ccgtttcgac gaagctatca tcaactatgt acgtcgtaat tacggttctc tgatcggtga
     9301 agccaccgca gaacgtatca agcacgaaat cggttcggct tacccgggcg atgaagtccg
     9361 tgaaatcgaa gttcgtggcc gtaaccttgc agaaggtgtt ccacgcggtt ttaccctgaa
     9421 ctccaacgaa atcctcgaag cactgcagga accgctgacc ggtattgtga gcgcggtaat
     9481 ggttgcactg gaacagtgcc cgccggaact ggcttccgat atctccgagc gcggcatggt
     9541 gctcaccggt ggtggcgcac tgctgcgtaa ccttgaccgt ttgttaatgg aagaaaccgg
     9601 cattccagtc gttgttgctg aagacccgct gacctgtgtg gcgcgcggtg gcggcaaagc
     9661 gctggaaatg atcgacatgc acggcggcga cctgttcagc gaagagtaat cggatgcagg
     9721 caggggaagt gtctgtttac cctgcctggt ctgatacgag aatacgcata acttatgaag
     9781 ccaattttta gccgtggccc gtcgctacag attcgcctta ttctggcggt gctggtggcg
     9841 ctcggcatta ttattgccga cagtcgcctg gggacgttca gtcaaatccg tacttatatg
     9901 gataccgccg tcagtccttt ctactttgtt tccaatgctc ctcgtgaatt gctggatggc
     9961 gtatcgcaga cgctggcctc gcgtgaccaa ctagaacttg aaaaccgggc gttacgtcag
    10021 gaactgttgc tgaaaaacag tgaactgctg atgcttggac aatacaaaca ggagaacgcg
    10081 cgtctgcgcg agctgctggg ttccccgctg cgtcaggatg agcagaaaat ggtgactcag
    10141 gttatctcca cggttaacga tccttatagc gatcaagttg ttatcgataa aggtagcgtt
    10201 aatggcgttt atgaaggcca gccggtcatc agcgacaaag gtgttgttgg tcaggtggtg
    10261 gccgtcgcta aactgaccag ccgcgtgctg ctgatttgtg atgcgaccca cgcgctgcca
    10321 atccaggtgc tgcgcaacga tatccgcgta attgcagccg gtaacggttg tacggatgat
    10381 ttgcagcttg agcatctgcc agcgaatacg gatattcgtg ttggtgatgt gctggtgact
    10441 tccggtctgg gcggtcgttt cccggaaggc tatccggtcg cggttgtctc ttccgtaaaa
    10501 ctcgataccc agcgcgctta tactgtgatt caggcgcgtc cgactgcagg actgcaacgt
    10561 ttgcgttatc tgctgctgct gtggggcgcg gatcgtaacg gcgctaaccc gatgacgccg
    10621 gaagaggtgc atcgtgttgc caatgaacgt ctgatgcaga tgatgccgca ggtattgcct
    10681 tcgccagacg cgatggggcc aaagttacct gaaccggcaa cggggatcac tcagccgact
    10741 ccgcagcaac cggcgacagg aaatgcagtt actgcgcctg ctgcgcccac acagcccgct
    10801 gctaatcgct ctccacaaag ggctacgccg ccgcaaagtg gtgctcaacc gcctgcgcgt
    10861 gcgccgggag gacaatagtg gcgagctatc gtagccaggg acgctgggta atctggctct
    10921 ctttcctcat tgcgctgttg ctgcagatca tgccctggcc ggataacctg attgttttcc
    10981 ggccaaactg ggtgttactc atcttgttgt attggatcct ggccttgccc catcgcgtaa
    11041 atgtgggcac aggttttgtg atgggtgcca tactggatct gatcagcggc tcgacgcttg
    11101 gcgtacgcgt attggcgatg agcatcattg cttacctggt ggcgctgaaa taccagcttt
    11161 tccgcaacct cgcattatgg cagcaggcgc tggtcgtcat gttgctttcg ctggtggtgg
    11221 atattattgt tttctgggca gagtttttag tgattaacgt ctctttcaga ccggaagtgt
    11281 tctggagtag tgtagtcaat ggcgttctct ggccgtggat tttcttgctg atgcgtaaag
    11341 tccgtcagca gtttgcagtg caataaaggt ttctatgact tctctgtatt tagcttccgg
    11401 ttctccgcgt cgtcaggagt tacttgcgca acttggcgtg acctttgaac gtattgttac
    11461 gggcattgag gagcagcgtc agccgcagga gagcgcgcag cagtatgttg tgcgtctggc
    11521 gcgcgagaaa gcacaggcgg gtgtcgcgca aacggcgcag gatctcccgg tgctgggtgc
    11581 ggatactatc gttatcctga acggagaagt gctcgagaaa ccgcgcgatg cagagcatgc
    11641 ggcgcagatg ttgcgcaaat tatcgggtca gacccatcag gtgatgacgg cagtggcgtt
    11701 ggccgacagc cagcacattc tcgattgcct ggtggtcacc gatgtgactt tcagaacgtt
    11761 aacagacgaa gacatcgcgg gctatgtcgc cagcggcgaa ccgttagata aagcaggtgc
    11821 atacggtatt caggggctgg gtggctgttt tgtcaggaag ataaatggca gctttcacgc
    11881 cgtagtcggc ttaccgctgg ttgaaacgta tgaattatta agtaatttta acgcactgcg
    11941 tgagaaaagg gataaacatg acggctgaat tgttggtaaa cgtaacgcct tcggaaacgc
    12001 gagtggcgta tattgatggc ggtattctgc aggaaattca tattgaacgt gaggcgcgac
    12061 gcggaatagt aggcaatatc tacaagggtc gtgtaagtcg tgtacttccg ggtatgcagg
    12121 cggcttttgt agatattggg ctggataaag ccgcgtttct tcatgcatct gacatcatgc
    12181 cgcacaccga atgtgtggcg ggtgaagaac aaaagcaatt cacggtgcgc gacatctcgg
    12241 aactggtccg tcaggggcaa gatctgatgg tgcaggtggt gaaagatccg cttggcacta
    12301 aaggtgcgcg cctgaccacc gatatcacgc tgccttctcg ctatctggtg tttatgccag
    12361 gggcttctca cgttggggta tcccaacgta ttgaaagcga atcagaacgt gaacgcctga
    12421 aaaaagtggt cgcagagtat tgcgacgagc agggcgggtt tatcatccgt actgcagcgg
    12481 aaggggttgg cgaggctgaa ctggcctccg atgccgctta tctgaaacgc gtctggacca
    12541 aagttatgga gcgtaaaaaa cgcccgcaga cccgttatca gctgtacggc gaactggcgc
    12601 tggcgcagcg tgttctgcgt gatttcgccg acgccgaact cgaccgcatt cgcgttgact
    12661 cacgcctgac ttacgaagca ttgctggagt ttacctcgga gtacattccc gagatgacca
    12721 gcaagctgga gcattacacc ggacgccagc cgattttcga tctctttgat gtcgaaaacg
    12781 aaattcagcg agcgctggaa cgcaaagtag aactgaaatc cggtggttat ctgattatcg
    12841 accagaccga agcgatgacc accgtggaca tcaataccgg ggcgtttgtc ggtcatcgca
    12901 atctggacga caccattttc aataccaata ttgaagcgac gcaggctatc gctcgccagt
    12961 tacggttgcg caatctgggc gggattatca ttattgattt catcgacatg aataatgaag
    13021 atcaccgtcg ccgcgtgctg cactcgctgg agcaggcgtt gagcaaagat cgggtgaaaa
    13081 ccagcgttaa tggtttttcg gcgctggggc tggtggagat gacgcgtaaa cgcacccgcg
    13141 aaagcattga gcacgtactg tgtaacgaat gcccaacctg ccacggtcgc ggaacggtga
    13201 aaaccgtgga aacggtatgc tatgaaatca tgcgcgagat tgttcgtgtc caccatgctt
    13261 acgactccga ccgtttcctg gtctatgctt ctccggcagt agccgaagcc ttgaaaggcg
    13321 aagagtcaca ctcgctggcg gaagtggaaa ttttcgttgg caaacaggtt aaagtacaaa
    13381 ttgaaccgct ctataaccag gagcagtttg acgtcgtaat gatgtaaaca gatgctgggc
    13441 cgccatccgg caaagggttt ttgagtcaca tttttagcag acaaggagtg acgggtgagg
    13501 cgattgccgg ggattttact gcttactgga gccgcgctcg ttgtgatcgc tgccctgctg
    13561 gttagcggcc tgcgtattgc tttaccgcat cttgacgcct ggcgtccgga aatcctcaac
    13621 aaaatagaat ccgcgactgg catgccggta gaagccagtc agctctcagc cagctggcag
    13681 aattttggtc cgacgcttga agcgcacgac atccgtgcag aactaaaaga tggcggcgaa
    13741 ttttcggtta aacgcgttac tctggcgctg gatgtctggc agagcctgtt acatatgcgc
    13801 tggcagtttc gcgacctcac tttctggcag ctgcgctttc gcaccaacac tcctatcacc
    13861 agcggcggtg gtaatgatag cctggaagcc agtcacatca gcgatctgtt tcttcgtcag
    13921 ttcgaccatt tcgatctccg cgacagtgaa gtcagtttcc tgacgccatc cggtcagcgc
    13981 gccgagctgg cgatcccaca gctcacctgg ctgaacgatc cacgtcgaca ccgtgcggaa
    14041 ggcctggtaa gcctctccag ccttaccgga cagcacggcg tgatgcaggt gcgtatggat
    14101 ttgcgcgatg atgaggggtt gttaagcaat ggtcgcgtct ggctccaggc ggatgacatc
    14161 gacctgaagc cgtggctcgg taaatggatg caggacaata ttgcgctgga aacggcacag
    14221 ttctcccttg aaggctggat gacgatcgac aaaggcgatg taaccggcgg tgacgtctgg
    14281 ctgaaacagg gcggtgccag ctggttgggc gagaaggaaa cacatacgct gtcggtggat
    14341 aatctgactg cgcatattac gcgtgaaaat ccgggatggc agttctctat tcctgataca
    14401 cggatcacga tggacggcaa accctggccg agcggagcat tgacgctggc ctggataccg
    14461 gaacaggacg ttggcggcaa agacaataaa cgcagtgacg aactccggat tcgcgccagt
    14521 aatctggagc tggcaggcct ggagggcgta cgcccgctgg tcgcgaaact ttcacctgca
    14581 ctgggtgatg tttggcgctc cacacaaccg agcggcaaga ttaacactct ggcgctggat
    14641 atcccgcttc aggctgcaga caagacccgt tttcaggcgt cgtggagcga tctggcctgg
    14701 aagcaatgga aattattacc gggtgcggaa cacttctccg ggacgctttc cggcagcgta
    14761 gaaaatggtt tgcttaccgc gtcgatgaag caggcaaaga tgccttacga aacggtattc
    14821 cgtgcgccac tggaaatcgc cgacggccag gctacgataa gctggctgaa caatgacaaa
    14881 ggtttccagc tggatgggcg taatattgac gttaaagcca aagccgtcca tgcgcgcggc
    14941 ggttttcgtt acctgcaacc tgctaacgat gaaccctggc tgggtattct ggctggcatc
    15001 agtaccgatg atggttcaca agcctggcgc tatttcccgg aaaacttgat gggtaaagac
    15061 ctggttgatt acttaagtgg cgcgattcag ggcggtgaag cggataacgc gacgctggtt
    15121 tatggtggca atccgcaact cttcccctat aaacacaacg aaggtcagtt tgaagtgctg
    15181 gtaccgctgc gcaacgcgaa gtttgccttc cagccggact ggcctgcatt aactaacctt
    15241 gatattgaac tggactttat taacgacggt ttatggatga aaaccgatgg cgttaatttg
    15301 ggcggcgtgc gcgcgagtaa tctcaccgca gtgatccctg actactcaaa agaaaaactg
    15361 ctgattgacg ctgacattaa aggtccgggt aaagccgttg gcccttactt tgatgagaca
    15421 ccgctgaaag attccctggg tgcgaccctg caagaactcc agctcgacgg cgatgtgaat
    15481 gctcgcttac atcttgatat cccgctgaac ggcgagttgg taaccgcgaa aggtgaagtg
    15541 acgctgcgta ataacagtct gtttatcaaa ccactcgaca gcaccctgaa aaatttgagc
    15601 ggtaaattca gctttatcaa tggcgatctg caaagtgaac cactgacagc aagctggttt
    15661 aatcagccgt tgaacgtgga tttttccacc aaagaagggg caaaagccta ccaggtcgcg
    15721 gtgaatctca acggtaactg gcaaccagcg aaaaccggct ttctgcctgc agcggtgaac
    15781 gaagcattaa gtggcagcgt ggcgtgggat ggtaaagtgg gcattgagct gccttatcat
    15841 gctggcgcga cgtataacgt agagctgaac ggcgatttga agaatgtgag cagtcactta
    15901 ccttcaccgt tagccaaacc tgcgggtgaa ccactaccgg taaacgttaa ggttgatggc
    15961 aatctcaaca gctttgaatt aaccggacag gctggtgcgg ataaccattt caatagccgc
    16021 tggttgctcg gtcaaaagct gacgctcgat cgtgctattt gggcggcaga cagtaaaacg
    16081 ctcccgccgt tgccggaaca aagtggcgtt gaactcaata tgccgccgat gaatggtgcc
    16141 gagtggctgg ccctgttcca gaaaggcgcg gcggagagtg tcggtggtgc agcgagtttc
    16201 ccacaacaca taacgttacg tacgcctatg ttgtcgctgg gaaatcagca atggaataac
    16261 ctgagtattg tttcgcaacc gacggcaaat ggcaccctgg ttgaggcgca agggcgtgaa
    16321 atcaacgcca cgctggcgat gcgtaataac gcaccgtggc tggcgaatat caaatatctt
    16381 tattacaacc caagcgtggc gaaaactcgt ggtgattcaa cacagtcatc acctttcccg
    16441 acaacggaac gcattaactt ccgtggctgg ccagacgcac aaatacgatg cgcagagtgc
    16501 tggttctggg ggcaaaaatt cggtcgcatt gacagtgata tcaccatttc tggcgatacg
    16561 ttaacgctga ctaatggact gattgatact ggttttgcgc ggctcactgc cgagggtgaa
    16621 tgggttaaca atccggggaa tgaacgaacc tcgctgaaag gaaaactgcg cgggcagaaa
    16681 attgatgccg ccgcagaatt ttttggtgtc acgacgccca tacgccagtc gtcatttaat
    16741 gtggattacg atttacactg gcgcaaagca ccctggcagc cagatgaggc gacgttgaat
    16801 ggcatcattc atactcaact gggtaaaggc gaaattaccg aaatcaatac cggacatgcc
    16861 gggcaattgc tgcgcttatt gagcgtagat gccctgatgc gtaagctgcg ttttgatttc
    16921 agagacactt ttggcgaagg gttctatttt gactccattc gcagcaccgc gtggattaaa
    16981 gacggcgtta tgcacaccga cgacacgctg gtggatggcc tggaagcgga catcgctatg
    17041 aaagggtcgg taaatctggt acgtcgcgac ctgaatatgg aagcggttgt cgcaccagag
    17101 atttctgcga cggtcggcgt ggctgcggcc tttgcggtta accccattgt tggcgcggca
    17161 gtgtttgccg ccagtaaagt gctgggaccg ctgtggagca aagtctccat tttgcgctat
    17221 cacatttcgg gtccgctgga cgatccgcaa atcaacgaag tgttgcgaca accgcgcaaa
    17281 gaaaaagcgc aatgatttga cgagggcgcg taattgcccc aatctcatat gataatcgtt
    17341 gccaaaggcc aacgagccag aacataaccg taggccggac agggcgttca cgccgcatcc
    17401 ggcagccgta aaaaatcctc tactgcagta actaacgagt agcaaaaacg atgagtctga
    17461 acctggtaag tgaacaattg ctagcggcga acggcctgaa acatcaggac ttgttcgcga
    17521 tcctcggtca actggccgaa cgtcgccttg attatggcga tctctatttt cagtcgagct
    17581 atcacgaatc ctgggtttta gaagaccgta ttattaaaga tggttcttac aacatcgatc
    17641 agggcgttgg cgtgcgtgca atcagtggtg aaaaaaccgg atttgcttat gctgaccaaa
    17701 tcagcctgct ggcgctggaa cagagtgcac aagcggcgcg caccatcgtc cgtgatagtg
    17761 gtgatggcaa agtacagacg ctgggcgcgg tagagcatag cccgttatat acctcggtag
    17821 atccgctgca aagcatgagc cgtgaagaga agttggatat tctgcgtcgc gtcgataagg
    17881 ttgcccgcga agcggacaag cgcgtacagg aagtgactgc cagcctcagt ggtgtctatg
    17941 aattaattct ggttgcggcc accgacggca cactagcggc ggatgtccgt ccgctggtgc
    18001 gtctttccgt gagcgttttg gtcgaagaag atggcaaacg cgaacgcggt gccagtggtg
    18061 gtggcggtcg ttttggttat gagttcttcc ttgccgatct cgacggcgaa gtccgtgcgg
    18121 atgcgtgggc aaaagaagca gtgcgtatgg cgctggtcaa tctttctgcc gttgctgcac
    18181 cagcgggcac catgccggta gtacttggcg caggttggcc gggcgtgctg ttgcatgaag
    18241 cggtgggtca cggtctggaa ggcgacttca accgccgtgg cacttcagta tttagtggac
    18301 aggtcgggga gttggtggct tcagaactgt gtaccgtggt tgatgatggc acgatggtcg
    18361 atcgccgtgg ttcggtggcg attgatgacg aaggtacgcc aggccagtac aacgtgctga
    18421 ttgagaacgg cattctgaaa ggctacatgc aggataaact caacgcgcgt ttgatgggga
    18481 tgacgccgac tggcaacggt cgccgtgaat cctacgctca tctgcccatg ccgcgtatga
    18541 ccaacaccta tatgctgccg ggtaaatcga ccccgcagga aattattgaa tccgttgagt
    18601 acggtatcta tgcaccgaac tttggtggcg gccaggtgga tatcacctcc ggcaaattcg
    18661 ttttctccac ctcagaagcg tatcttattg aaaacggtaa agtaactaag ccggtgaaag
    18721 gcgcaacgtt gattggttcc ggtatcgaaa ccatgcagca gatttcgatg gttggcaacg
    18781 acctgaaact ggataacggc gtgggtgtct gcggtaaaga agggcaaagt ttgccggttg
    18841 gcgtgggcca gccaacgctg aaagttgata acctgactgt tggcggtact gcgtaataat
    18901 ttcattattt tcagaaggat aattaatcct tctccgtgta cgaacggtcc cctcgcccct
    18961 ctggggttag ggtgagggga acccgttggc acaggtttgt acaacgtaac agtacaatat
    19021 taattacttc tctttcccgc gcccgtgcat ctcctgaaac aatttaccga cctcaacaaa
    19081 ataatccgtt agcgagttga tcacgacttg taccttcagc ggcagcttat ctttttcggt
    19141 atataacgca taaaccgggc gtggatctga ctggtaacgt ggcagcagga tctccagttc
    19201 cccgcgattg atctcgttga tcacccacat caacggcacg taggcgatcc cggcacccgc
    19261 cgtcagccag cgcaccagcg tcatcggatc attagtcaca aatctccctt gcgggatcag
    19321 gcgagtcgag atcccttccg gtgcgatcag ttcaaattca ttgtcgggtc gcacgctgta
    19381 ttcaagccat gagtgactac ttaaatcggc gggtttttcc ggtatgccgt attgtgtgag
    19441 ataacttttt gcggcgcaca ccaccattgg catcgcgccc agacggcggg aaaacaggct
    19501 ggaatcttgc aacgcgccga cgcggatcac cacatcaaga ccgtcggcaa tcaggtcggg
    19561 ggcaggaatt ccggtaacca gattgacgct caaacctggg tattctttca gcatttttgc
    19621 tgtcagcccg gcgagaacat tttgtgccat agttgaagaa cagccaatgc gcagcgtccc
    19681 gatgggggtg ttattgaagg catacagttg ctcatgaaca tcctgcactt catggagcat
    19741 ccgacggcag ccctggtagt aaattctgcc ggcttcagtc aggccaatgc tgcgtgtact
    19801 acggtttaac agctttacct gtaactcatc ttccagtttt gacaccgtct gactgatgga
    19861 cgaaacgctc atctgtagct gtctggcggc ggcggtaaaa gagccaaatt caactacttt
    19921 ggcaaacacc gacatgcgtt ttagtcgttc cattattcac tctgacttaa aagtgattta
    19981 gatcacataa tatagataac agcataacag ttacgctaat atattaaata tcaatctaca
    20041 gcaatgttgc tctcgcccgg ctttccatgc ccttctctgc ggcgacagat gctgaaaata
    20101 ataacgcctg ctctctctat acaaccaagg tcaacatgag tctgtttccc gttatcgtgg
    20161 tgtttgggct gtccttccca ccgatatttt tcgaattgct tttatcactg gcgattttct
    20221 ggctggtgcg ccgggtactt gtgccaacag gtatttacga ctttgtctgg catccggcgt
    20281 tgttcaacac cgcgctctat tgctgcttgt tttatttgat atcgcgactg ttcgtttgag
    20341 gttgaagtga aaacactaat aagaaaattc tcccgtacgg ccatcacggt cgtattagtc
    20401 attctggcct tcatcgcaat ttttaatgcc tgggtctatt acaccgaatc cccgtggacg
    20461 cgtgacgcgc gctttagcgc tgacgtcgtt gcgatcgcgc cggacgtttc tggactcatt
    20521 acccaggtga atgttcatga taaccagctg gtgaaaaaag gacaggtact gttcaccatc
    20581 gaccagccgc gctatcaaaa ggcgcttgag gaagcgcaag ccgatgttgc ttattatcag
    20641 gtactggcac aggagaaacg ccaggaggcc ggacgtcgta accgtctcgg tgtgcaggcg
    20701 atgtctcgcg aagagatcga ccaggccaac aacgtactac aaacagttct gcatcagtta
    20761 gcgaaagcgc aggcgacccg cgatctggca aaactggatc ttgaacgtac agtcatccgc
    20821 gcgccagcag atggctgggt gaccaacctc aacgtctata ccggtgagtt tattactcga
    20881 ggatcaacgg cggttgcgct ggtgaaacag aactccttct atgtactggc ctatatggaa
    20941 gaaactaagc tggaaggggt gcgccctggg tatcgtgcgg agatcacgcc gcttggcagt
    21001 aacaaagtgc tgaaagggac tgttgatagt gttgccgcag gggtcaccaa tgccagcagc
    21061 acgcgtgacg acaaagggat ggcgaccatc gactctaacc ttgaatgggt acgtttggcg
    21121 caacgtgttc cggtacgtat tcgtctcgac aaccagcaag agaacatctg gcctgcgggc
    21181 accactgcta cggtggtagt cactggcaaa caagatcgcg acgaaagtca ggattcgttc
    21241 ttccgtaaaa tggctcatcg cctgcgtgag tttggttaat cacgatgggt attttctcca
    21301 ttgctaatca acatattcgc tttgcggtaa aactggcgac cgccattgta ctggcgctgt
    21361 ttgttggctt tcacttccag ctggaaacgc cacgctgggc ggtactgaca gcggcgattg
    21421 ttgccgctgg tccggccttt gctgcgggag gtgaaccgta ttctggcgct attcgctatc
    21481 gtggcttttt gcgcatcatc ggcacattta ttggctgtat tgccggactg gtaatcatca
    21541 ttgcgatgat ccgagcacca ttattgatga ttctggtgtg ctgtatctgg gccggttttt
    21601 gtacctggat atcctcgctg gtacgaatag aaaactcgta tgcgtggggg ctggccggtt
    21661 ataccgcgct gatcattgtg atcaccattc agccggaacc attgcttacg ccgcagtttg
    21721 ccgtcgaacg ttgtagcgag atcgttatcg gtattgtgtg tgcaattatg gcggatttgc
    21781 tcttttctcc gcgatcgatc aaacaagaag tggatcgaga gctggaaagt ttgctggttg
    21841 cgcaatatca attaatgcaa ctctgtatca agcatggcga tggtgaagtt gtcgataaag
    21901 cctggggcga tctggtgcga cgtactacgg cgttacaagg tatgcgcagc aacctgaata
    21961 tggaatcttc ccgctgggcg cgggccaatc gacgtttaaa agcgatcaat acgctatcgc
    22021 tgacgctgat tacccaatcc tgcgaaactt atcttattca gaatacgcgc ccggaattga
    22081 tctctgatac tttccgcgaa ttttttgaca cgccggtaga aactgcgcag gacgtccaca
    22141 agcagctcaa acgcctgcgg agagtgatcg cctggaccgg ggaacgggaa acgcctgtca
    22201 ccatttatag ctgggtcgcg gcggcaacgc gttatcagct tctcaagcgc ggcgttatca
    22261 gtaacacaaa aatcaacgcc accgaagaag agatcctgca aggcgaaccg gaagtaaaag
    22321 tagagtcagc cgaacgtcat catgcaatgg ttaacttctg gcgaaccaca ctttcttgca
    22381 ttctgggcac gcttttctgg ctgtggacgg gctggacttc cggcagtggt gcaatggtga
    22441 tgattgcggt agtgacgtca ctggcaatgc gtttgccgaa tccacgcatg gtggcgatcg
    22501 actttatcta tgggacgctg gcggcgctgc cgttagggct gctctacttt ttggtgatta
    22561 tccctaatac ccaacaaagc atgttattgc tgtgtattag cctggcagtg ctgggatttt
    22621 tcctcggtat agaagtacag aaacggagat tgggctcgat gggggcgttg gccagcacca
    22681 taaacattat cgtgctggat aacccgatga ctttccattt cagtcagttt ctcgacagcg
    22741 cattagggca aatcgtcggc tgtgtgctcg cgttcaccgt tattttgctg gtgcgggata
    22801 aatcgcgcga caggactgga cgtgtactgc ttaatcagtt tgtttctgcc gctgtttccg
    22861 cgatgactac caatgtggca cgtcgtaaag agaatcacct cccggcactt tatcagcagc
    22921 tgtttttgct gatgaataag ttcccggggg atttgccgaa atttcgcctg gcgctgacga
    22981 tgattatcgc gcaccagcgc ctgcgtgatg cgccgattcc ggttaacgag gatttatcgg
    23041 cgtttcaccg gcaaatgcgc cgcacagcag accatgtgat atctgcccgt agcgatgata
    23101 aacgtcgtcg gtactttggc cagttgctgg aagaactgga aatctaccag gaaaagctac
    23161 gcatctggca agcgccaccg caggtgacgg aaccagtaca tcggcttacg ggtatgctcc
    23221 ataagtatca acatgcgttg accgatagtt aagtcaaaac cggcgcccga agcgtcggtt
    23281 ttttcatggc tatacttagc gatgaacggc agaactcgcc gcgaaacgtg acggtggcaa
    23341 cagatgaata tttatacctt tgattttgat gagattgaga gtcaggagga tttttatcgt
    23401 gactttagcc aaacctttgg tctggcgaaa gataaggtac gcgatctcga ctcactatgg
    23461 gatgtgttaa tgaacgatgt cctgccgcta ccacttgaga ttgaatttgt tcatctggga
    23521 gagaaaacgc gtcgccgttt tggcgcgtta atattgctgt ttgatgaggc agaggaagag
    23581 ctggaagggc atttgcgttt taatgttcgt cattagcgca aaaaaagccc ccgaaccggg
    23641 ggcaatatcg tcggacaaga cgatgagggt ttatttgtac agttcagccg tagcgtgcca
    23701 ggtgtcacca ctacgagctt cagtaatctg gtaggccgtt gcgcctttct cttccgcttt
    23761 tttgttcagc atttcacgca tatccattgg cgaagacgcc acaccactta cggatacggt
    23821 cccgattgct tcacgatttt gtgcttgtgc agcatcaatg gagtcggcag cgaatgcacc
    23881 gaaagagaga acagaaagca cacttaatgc agcaacagtg gttttgattt tcatgatttt
    23941 tacctcgaca taatctttta gctgggcctt tgtttcgtga cccatatcac aaaatcaagt
    24001 atacactaat tattgaacta attaatacca cgctaactgt ttcagttgat actaagtgtt
    24061 aaaaatgtga attttaataa caaaaaagaa ttaaaaacac gatcatttat tgttaattat
    24121 agtattttca attagataat gaattttgc
//
LOCUS       DAZFJL010000074        23715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFJL010000074 DAZFJL010000000
VERSION     DAZFJL010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.074"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            258..2279
                     /gene="uvrB"
                     /locus_tag="ACHJ8C_002780"
     CDS             258..2279
                     /gene="uvrB"
                     /locus_tag="ACHJ8C_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO5552614.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(2471..3379)
                     /gene="yvcK"
                     /locus_tag="ACHJ8C_002781"
     CDS             complement(2471..3379)
                     /gene="yvcK"
                     /locus_tag="ACHJ8C_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO5552615.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     regulatory      3644..3788
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            3776..4765
                     /gene="moaA"
                     /locus_tag="ACHJ8C_002782"
     CDS             3776..4765
                     /gene="moaA"
                     /locus_tag="ACHJ8C_002782"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO5552616.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            4787..5299
                     /gene="moaB"
                     /locus_tag="ACHJ8C_002783"
     CDS             4787..5299
                     /gene="moaB"
                     /locus_tag="ACHJ8C_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO5552617.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            5302..5787
                     /gene="moaC"
                     /locus_tag="ACHJ8C_002784"
     CDS             5302..5787
                     /gene="moaC"
                     /locus_tag="ACHJ8C_002784"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO5552618.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            5780..6025
                     /gene="moaD"
                     /locus_tag="ACHJ8C_002785"
     CDS             5780..6025
                     /gene="moaD"
                     /locus_tag="ACHJ8C_002785"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO5552619.1"
                     /translation="MINVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHSLTDGDEVAFFPPVTGG"
     gene            6027..6479
                     /gene="moaE"
                     /locus_tag="ACHJ8C_002786"
     CDS             6027..6479
                     /gene="moaE"
                     /locus_tag="ACHJ8C_002786"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO5552620.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            6616..7320
                     /gene="ybhL"
                     /locus_tag="ACHJ8C_002787"
     CDS             6616..7320
                     /gene="ybhL"
                     /locus_tag="ACHJ8C_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO5552621.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            7527..8240
                     /gene="ybhM"
                     /locus_tag="ACHJ8C_002788"
     CDS             7527..8240
                     /gene="ybhM"
                     /locus_tag="ACHJ8C_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO5552622.1"
                     /translation="MESYSQNSNKVDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPQMYVAIRLLLLSMYGLSKDINKINTAISGVIYLFLISLVAIVVASLAP
                     VSAIIVVFSTAGTMFLISMLAGLLFKFDPGSHRFIIMMTLAGLALVIIVNATLMSERP
                     IWVISCLMIVLWSGIISHGRNKLLELTGKSHSEELWSPVRCAFVGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(8276..9232)
                     /gene="ybhN"
                     /locus_tag="ACHJ8C_002789"
     CDS             complement(8276..9232)
                     /gene="ybhN"
                     /locus_tag="ACHJ8C_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO5552623.1"
                     /translation="MSKSHPRWRLAKKLLTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGVIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVSYFFVVGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(9232..10473)
                     /gene="clsB"
                     /locus_tag="ACHJ8C_002790"
     CDS             complement(9232..10473)
                     /gene="clsB"
                     /locus_tag="ACHJ8C_002790"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO5552624.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVGP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(10470..11231)
                     /gene="ybhP"
                     /locus_tag="ACHJ8C_002791"
     CDS             complement(10470..11231)
                     /gene="ybhP"
                     /locus_tag="ACHJ8C_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO5552625.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            11364..11774
                     /gene="ybhQ"
                     /locus_tag="ACHJ8C_002792"
     CDS             11364..11774
                     /gene="ybhQ"
                     /locus_tag="ACHJ8C_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO5552626.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFVL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(11736..12842)
                     /gene="ybhR"
                     /locus_tag="ACHJ8C_002793"
     CDS             complement(11736..12842)
                     /gene="ybhR"
                     /locus_tag="ACHJ8C_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5552627.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMSVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(12853..13986)
                     /gene="ybhS"
                     /locus_tag="ACHJ8C_002794"
     CDS             complement(12853..13986)
                     /gene="ybhS"
                     /locus_tag="ACHJ8C_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5552628.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLIVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(13979..15715)
                     /gene="ybhF"
                     /locus_tag="ACHJ8C_002795"
     CDS             complement(13979..15715)
                     /gene="ybhF"
                     /locus_tag="ACHJ8C_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO5552629.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDAALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEIDINETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(15708..16703)
                     /gene="hlyD"
                     /locus_tag="ACHJ8C_002796"
     CDS             complement(15708..16703)
                     /gene="hlyD"
                     /locus_tag="ACHJ8C_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO5552630.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLVAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPNKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(16706..17377)
                     /gene="cecR"
                     /locus_tag="ACHJ8C_002797"
     CDS             complement(16706..17377)
                     /gene="cecR"
                     /locus_tag="ACHJ8C_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO5552631.1"
                     /translation="MNNSAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     SDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            17606..18967
                     /gene="rhlE"
                     /locus_tag="ACHJ8C_002798"
     CDS             17606..18967
                     /gene="rhlE"
                     /locus_tag="ACHJ8C_002798"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO5552632.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPTKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGRGQGGGRGQQQSRRAESGAKSGNAKPAEKPSRRLGDAKPAGEQQRRRRPRKPA
                     AAQ"
     gene            complement(19200..19400)
                     /locus_tag="ACHJ8C_002799"
                     /pseudo
     CDS             complement(19200..19400)
                     /locus_tag="ACHJ8C_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR domain-containing protein"
     gene            19504..21654
                     /gene="dinG"
                     /locus_tag="ACHJ8C_002800"
     CDS             19504..21654
                     /gene="dinG"
                     /locus_tag="ACHJ8C_002800"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO5552633.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IISDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            21682..22644
                     /gene="ybiB"
                     /locus_tag="ACHJ8C_002801"
     CDS             21682..22644
                     /gene="ybiB"
                     /locus_tag="ACHJ8C_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO5552634.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSYI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLMATGEAATISDGLARVNQAF"
     gene            complement(22823..23485)
                     /locus_tag="ACHJ8C_002802"
     CDS             complement(22823..23485)
                     /locus_tag="ACHJ8C_002802"
                     /inference="COORDINATES: protein motif:HMM:NF022337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFO5552635.1"
                     /translation="MFPKIPLWQIPLALLLSVIALFFFYLLFLALYSLIRGLTDKKLK
                     KESIHKTLHHAPDNTPNYAPPLFNYKATSKKSHTLQYDAFQFYQKKNFMSNRERRFFK
                     LIYDEFHQDFFVFSQVRMVDIIVPIFDKNTQYNSFIKAFRSISQYHIDFLIVNKNDFS
                     IICAIELDDYSHDLPDRVDRDQKVNMIFKFTGITLLRSRNLEELLNKIRHHIQHHNDE
                     IT"
BASE COUNT         5746 a         6082 c         6249 g         5638 t
ORIGIN      
        1 gcatctcttg ccataaaact gtcatcaatc atcttgacaa atgttaaaaa agccgttgct
       61 ttggggataa cccggtaagg ccggagtttt atctcgccac agagtaaatc ttgttcatga
      121 ttgacagcgg agtttacgct gtaacagaaa tattatggtg atgaactgtt tttttatcca
      181 gtataatttg ttggcataat taagtacgac gagtaaaatt acatacctgc ccgcccaact
      241 ccttcaggta gcgactcatg agtaaaccgt tcaaactgaa ttccgctttt aaaccttctg
      301 gcgatcagcc ggaggcgatt cgacgtctcg aagaggggct ggaagatggc ctggcgcacc
      361 agacgttact tggcgtgact ggctccggga aaaccttcac cattgccaat gtcattgctg
      421 accttcagcg cccaaccatg gtacttgcgc ccaacaaaac gctggcagcc cagctgtatg
      481 gcgaaatgaa agaattcttc ccggaaaacg cggtggaata tttcgtctcc tactacgact
      541 actatcagcc ggaggcctat gtaccgagtt ccgacacctt cattgagaaa gatgcctcgg
      601 taaacgaaca tatcgaacag atgcgtttgt ccgccaccaa agcgatgctg gagcggcgtg
      661 atgtggttgt ggtggcgtct gtttctgcga tttatggtct gggcgatcct gatttatatc
      721 tcaagatgat gctccatctc acggtcggta tgattatcga tcagcgagcg atcctgcgcc
      781 gactggcgga gctgcaatat gctcgtaatg atcaagcatt ccagcgtggt actttccgtg
      841 ttcgtggtga ggtgattgat atcttcccgg cagaatcgga tgacattgca cttcgcgtgg
      901 agctgtttga cgaggaagtg gaacgattgt cgttatttga cccgctgacc gggcagattg
      961 tttccactat tccacgtttt accatctacc cgaaaacgca ctacgtcacg ccacgcgagc
     1021 gcatcgtcca ggcgatggag gagatcaaag aagagctggc cgccagacgt aaagtgctat
     1081 tggaaaacaa caaactgctg gaagagcagc ggctgaccca gcgtacccag tttgatctgg
     1141 agatgatgaa cgagctaggc tactgttcgg ggattgagaa ctactcgcgc ttcctctccg
     1201 gtcgtggacc gggtgagcca ccgccgacgc tgtttgatta cttacctgcc gatgggctgc
     1261 tggtggtcga tgaatctcac gtcaccattc cacaaattgg cggcatgtat cgcggtgacc
     1321 gggcgcgtaa agagacactg gtggagtacg gcttccgctt gccatcagcg ctggataacc
     1381 gcccgctgaa atttgaagag ttcgaagcat tagcgccgca aaccatctat gtttcggcga
     1441 cgccgggtaa ttacgagctg gaaaaatccg gtggcgatgt ggtggatcag gtggtgcgtc
     1501 caacaggctt actcgacccg attatcgaag tgcggccagt ggcaacacag gtggatgatc
     1561 ttctttcgga gattcgtcag cgagcggcaa ttaacgaacg cgtactggtt acaactctga
     1621 ccaagcggat ggcggaagat ctcactgaat atctcgaaga acacggtgag cgcgtgcgtt
     1681 atcttcactc agatatcgat accgtcgaac gtatggagat tatccgcgac ttgcgtctgg
     1741 gtgagtttga cgtattggta gggatcaact tactgcgcga aggtctggat atgccggaag
     1801 tttcgctggt ggcgatcctc gacgctgaca aagaaggctt cctgcgttcc gaacgttcgt
     1861 tgatccagac cattggtcgt gcggcacgta acgttaacgg taaagcgatt ctctacggcg
     1921 ataagatcac cccatcaatg gcgaaagcga ttggcgaaac cgaacgtcgc cgcgagaaac
     1981 agcagaagta caacgaggaa cacggcatta cgccgcaagg cttgaacaag aaagtggtcg
     2041 atatcttggc gctggggcag aacattgcca aaaccaaagc gaagggcaga ggaaaatcgc
     2101 gaccgattgt tgagccggat aatgtgccga tggatatgtc gcctaaagcg ttgcagcaga
     2161 aaatccatga actggaaggg ttgatgatgc aacacgcgca gaatctggag ttcgaagaag
     2221 cggcacaaat tcgtgaccag ttgcatcagc tgcgtgagtt gttcattgcg gcttcgtgac
     2281 aggataacga agaagacaga tgacaaaagc aaaattgcct gatgtgctac gcttatcagg
     2341 cctacatgat ctctgcaata cattgaatta gcgcggtttg taggccggat aaggctttca
     2401 cgccgcatcc ggcataaaca acgcgcactt tgtcttcaat aggaagccgg aattttcctt
     2461 cggctttccg ttaacctaaa gcctgtaatg ccttttccag cgcgctatgc aacaactggc
     2521 ggtcatgacg atacggaata tcgctggcct ccagtacctc ctgaatcaca atccgctctt
     2581 tcaccgccga gacatccact tttggcccga cgatgaccgc atcaataacc tttttaccga
     2641 catactgctc cataattgcc agcttgcttt ccagcttcaa attagccgca ggtaaactca
     2701 actcacgccc cagattgccg atatagacca tcggcgctgg cgtgcggcgt aatgcctgtg
     2761 cgatttcctt cagcagcaga attggcatca ggctggtata aaaactgcca ggcccaataa
     2821 tgatgagatc cgcttcattg atagcgtgaa ccgcctcacg ggttgcgggt acattaggcg
     2881 ttaacaataa ctcttgaatc ggcgtagtta actggtcgat attgacctcg ccgtaaactt
     2941 catgcccctg atcgtcaatc gccatcagat caacaggatg ctccgacatc ggaatcaaat
     3001 gtgcgtccac tttcagcaga ttgcgaatca gattgatcgc ttcaagaggt cgcacgctaa
     3061 ggtgatccag tgccttcagc atcaggtttc caaggttgtg tccggaaagt tcgccattac
     3121 cgccgaaacg atattcaaac attgcggaag cgacgctggg ttcagttatc agctggttga
     3181 ggcagttgcg catatcgccc caggcaatgc cgccttctga gcggcgaata cgccccgtcg
     3241 agccaccgtt atcggtggtg gtgacgatac ccgttaaacg agaacccaaa gacgaaagtg
     3301 atgagagaac gcgtcccagt ccatgccctc cgccgagagc aacgacacga tcaaggtcag
     3361 ccagcgtacg attgcgcata tagtttcctg gagtcagatt atccgcgcta cagtagcgca
     3421 aattgcgggg aaacagcaat taacctgcca atattgagga tacaaactat tttcttctac
     3481 ctctaaagga cgatgcacgc tatgcctccc tgatgatgta tatcaaagta aaaccgccat
     3541 tttcccttat tctgtagcga aatagcacga tcatgacgct atatacatga ttacatagcg
     3601 aaagtgtgga tggtaaaaat ctcatttaca cgctagtatc ggcttaacca ctaaacactc
     3661 tagcctctgc acctgggtca actgatacgg tgctttggcc gtgacaatgc tcgtaaagat
     3721 tgccaccagg gcgaaggaag aaatgacttc gcctcccgta tctggaaagg tgtacatggc
     3781 ttcacaactg actgatgcat ttgcgcgtaa gttttactac ttgcgcctgt cgattactga
     3841 tgtgtgtaac tttcgttgca cctactgcct gccggatggt tacaagccga gcggcgtcac
     3901 caataaaggc tttctgaccg tcgatgaaat tcgccgggtt acgcgcgcct tcgccagtct
     3961 gggcaccgaa aaagtccgtc tgacaggtgg tgagccatct ttacgccgcg actttaccga
     4021 tatcatcgcc gctgtgcgag aaaacgacgc tattcgccag atagcggtca ccaccaatgg
     4081 ttaccgtctg gagcgcgatg tggcgaactg gcgcgatgct ggacttactg gcatcaacgt
     4141 cagcgttgat agccttgacg ctcgccagtt tcatgccatt accgggcagg ataaattcaa
     4201 ccaggtcatg gcaggaatcg atgctgcatt tgaggccggt tttgagaagg ttaaagtcaa
     4261 taccgtgctg atgcgtgatg ttaatcatca ccagctcgat acctttctga actggatcca
     4321 gcatcgtccc atccagctgc gtttcatcga actgatggaa acgggcgagg gcagcgagct
     4381 cttccgtaag catcacatct ctggtcaggt tctgcgtgac gagctactgc gtcgcggctg
     4441 gatccaccaa ttacgtcaac gcagcgacgg tcccgcgcaa gtcttttgtc atccggatta
     4501 cgccggagag attggcctta tcatgccgta tgaaaaagac ttctgcgcca cttgcaaccg
     4561 cctgcgcgtt tcctccattg gtaaactcca tctctgcctg tttggtgaag gcggcgttaa
     4621 cctgcgcgat ctgctggaag acgataccca gcaacaggcg ctggaagcgc gtatttcagc
     4681 ggcgctgcgg gagaaaaaac agacccattt cctgcatcaa aataacaccg gtattacgca
     4741 aaacttatcg tacattggtg gctaaaacgt caaaaggaga gatcagatga gtcaggtaag
     4801 cactgaattt atcccgaccc gtattgctat tcttacggtt tctaatcgtc gcggtgaaga
     4861 agacgatacc tccggtcact atctgcgcga ctcggcgcaa gaagcgggcc atcacgttgt
     4921 cgataaagcc attgtgaaag aaaaccgcta cgctattcgc gctcaggtat ctgcgtggat
     4981 cgccagcgac gatgtacaag tggtgttgat tacgggtggt actggcctga cggaaggaga
     5041 tcaggctccc gaagcgctgc tgccgttgtt cgaccgtgaa gttgaaggtt ttggtgaagt
     5101 gttccggatg ttgtcgttcg aagagattgg cacttccacg ttgcaatctc gtgcggtagc
     5161 gggcgtcgct aacaaaacgc tgattttcgc catgccgggt tcgaccaaag cgtgccgtac
     5221 cgcatgggaa aatatcattg cgccgcagct ggatgcccgt acgcgtccgt gtaatttcca
     5281 tccacatttg aagaaataag tatgtcgcaa ctgacccata tcaacgccgc tggcgaagcg
     5341 cacatggtgg atgtctccgc caaagcggaa accgtgcgtg aagcgcgcgc cgaagcattt
     5401 gtcaccatgc gcagcgagac gctggcgatg attattgatg gtcgccacca caaaggcgac
     5461 gtatttgcca ctgcgcgtat cgccggtatt caggcggcaa aacgcacctg ggatctgatc
     5521 ccgctgtgtc acccgctgat gctcagcaaa gttgaagtca atttacaggc cgagccggag
     5581 cacaatcggg tacgtataga aaccttatgc cgcctgaccg ggaaaaccgg tgtcgaaatg
     5641 gaagcgttaa ccgcggcctc cgtggcggcg ctgaccattt atgacatgtg caaagcggtg
     5701 caaaaagata tggtgattgg tccggtacgt ttgctggcaa agagcggcgg caagtcgggt
     5761 gactttaagg tggaagcgga tgattaacgt tctttttttc gcccaggtgc gcgagttggt
     5821 gggaacagat gcaaccgaag tggctgcgga tttcccaacc gttgaagcgt tacgccaaca
     5881 tctggctgcg cagagcgatc gctgggcgct ggcgctggaa gatggcaaat tgctggctgc
     5941 tgtcaaccag acgctggtga gttttgacca ttcgctgact gacggcgacg aagtggcttt
     6001 cttcccgccg gtaaccggag gttaagatgg cagaaaccaa aattgttgtc ggcccgcagc
     6061 cgttcagcgt aggagaagag tacccgtggc tggctgagcg tgacgaagac ggtgcggtag
     6121 tcacctttac cggtaaggtg cgcaaccata acctgggcga cagcgtcaaa gcattaaccc
     6181 tcgaacacta tccgggaatg acagaaaaag cactggcaga aatcgttgat gaagcgcgta
     6241 accgctggcc gctggggcgc gtcacggtaa ttcaccgcat tggtgaatta tggccgggcg
     6301 atgaaattgt ttttgtcggt gttaccagtg cgcatcgcag cagtgcgttt gaagccgggc
     6361 agtttattat ggattatctc aaaacccgcg caccgttctg gaagcgcgaa gccacgccgg
     6421 aaggcgaccg ctgggtagaa gctcgggaga gcgatcagca ggcggcaaaa cgctggtagt
     6481 ttattgttag ccggataagg caccgttgcg tccggcaatt tcaccgctca gtacattcgt
     6541 ttacgctgcg tcgatgcaca gcctcatcat tttgcagtat ccttaagata ttccttatat
     6601 cttcaggaga aagtcatgga cagattccca cgttctgatt caatcgtaca accccgggct
     6661 ggcttgcaaa cttatatggc gcaagtctat ggctggatga ccgttggctt gttgctgaca
     6721 gcatttgttg cctggtatgc ggctaattcc gcggccgtga tggagctgtt gtttactaac
     6781 cgtgtctttt taatcggtct gatcatcgcg caattagcat tggttattgt gttatcagcg
     6841 atgattcaaa agctgagcgc aggtgtaacg acgatgctct ttatgcttta ttcggcgctg
     6901 acgggtctta cgctttccag tattttcatt gtctataccg ctgcttctat cgccagtact
     6961 ttcgtcgtta ctgccgggat gttcggcgca atgagcctgt acggttacac cacgaagcgc
     7021 gatctaagcg gctttggcaa tatgctgttt atggcgttaa tcggcattgt gctggcatcg
     7081 ctggttaact tctggctgaa aagcgaagca ttgatgtggg cagtcaccta catcggcgtg
     7141 attgtctttg tcggattgac ggcgtatgac acccagaaac tgaaaaatat gggtgagcag
     7201 attgatactc gcgacacgtc gaacctgcgc aaatattcca ttcttggcgc gttaaccttg
     7261 tatctggact tcatcaacct gttcctgatg ttgctgcgga tcttcggcaa ccgccgttaa
     7321 ttctttttca tcatcaatat cccggccatg tgtcgggatt ttttttctta ttaaaaacga
     7381 cacttaacaa gtaccaggta taaaatatgc cttatggacg agcggaagcg ttttctgtat
     7441 ccataaatgc aaaagtattt tgtatgcgtg gttttaaatt tattgatggt gaatattaat
     7501 gtttttttct aaggacaact aaaagaatgg aatcatactc gcaaaatagc aataaagtag
     7561 attttcagca cgaagccagg atattaaacg gcatatggct cattaccgct ttgggtttgg
     7621 tggcaaccgc agggctggcc tggggtgcta agtatctcga aattacggca acaaaatatg
     7681 attcaccgca aatgtatgtt gctataagat tattattact ttctatgtat ggcttaagta
     7741 aagatatcaa caagataaat accgccatct cgggcgtaat atatttgttt ttaatctctt
     7801 tggtggcgat tgtcgttgcg agtttagcgc ctgtatctgc cattatcgtc gtgttcagca
     7861 ctgcgggaac gatgttttta atcagtatgc tggcaggtct attgtttaaa tttgatcctg
     7921 gttctcaccg ttttatcatt atgatgacgt tggcagggtt ggccctggta atcatcgtga
     7981 atgccacatt aatgagtgaa cgacccattt gggtaataag ttgcttaatg attgtcttat
     8041 ggtcaggcat tatctcgcat ggacgaaata agctccttga actgacagga aaaagccata
     8101 gtgaagaatt atggagtccg gttcgttgtg cttttgtagg tgcattaaca ctctattact
     8161 attttatcgg cttctttggg attcttgccg ctatagcgat aacgcttgtc tggcaaaggc
     8221 atacgcggtt ttttcattag ccgtaaatgc acatactaaa gaacttaact atacttcaca
     8281 tcgccgcttc atttttcgcc cgtagcttct tcgcctggct ttccagcaac aggtaacaga
     8341 tcagcgccag cagcagtggg ataaagtaat acagcacacg gtaagcgagc agggcggcga
     8401 taattgtgcc tttggaggta tgctccccag ccagtagcgc gataaacacc gcttccagca
     8461 caccaattcc cgccggaata tgcacgatga cgccagcaat actactaacc agcaacacgc
     8521 ccactacaaa gaaatagctt acgctttgac caagtaacag ccagataatc gctcccatta
     8581 ccatccagtt aacgctggaa atcagcatct gggcgagggc gaatttccac gaaggcagca
     8641 ccagcttttg ccctttgatg gtcatatggc ggtgcttcgc gaaagcgcaa aaccacaaat
     8701 aaaccgcgat aatcatcagt aagccaatac cgagaatacg cagcgtagtt tgatcgacat
     8761 accagtgatc cggcaactcc accacgcctg cggtaaagat aacccccgct agcaaaatat
     8821 agcctagcca gttggtggta atactgagcg aaaaaatccg cgtaatagtg ctgcccggta
     8881 accccagtcg ggagtacaaa cgatagcgca taccaatgcc gccgacccag gtactgagtg
     8941 tcaggttgaa ggcgtagcag ataaacgaca ccagcatcac ctggcgcttc gccagtttgt
     9001 gaccgcagta gaaacgggcg agcaggtcgt agcaaccgta aatcagataa ctgacgacca
     9061 ccagcccgac cgcactaagc agcgcaacgc gattgtagtc gcggatgacc ttccagacct
     9121 cttcccagtc cacttttttg gcgtagacca ccagcaacac aatcaccgcg ataaaaaaca
     9181 gccaggtgag gagtttcttt gctaagcgcc agcgcgggtg tgatttactc atcagggttt
     9241 tacccccgtg ttttcagttt ctacccgatc ctgcgtttcc attgtcggtt gtgcgggggg
     9301 accaacctgc gccagacgtg gcgtgtgcgc cggaagccag cccaccagcg ccgggaagtg
     9361 gcgtaaaaag tggaacgcca gcacgctttt ggtcaggttc caccaggtgc gtttcggcag
     9421 catggtctca tcgacctgct gacaatctgc ggcgataatg ccgttcagat tatcgcgcag
     9481 cgtctggtta aaattacgat cgtggatgat gacatttgct tcgagattca gtgacaaact
     9541 gagcggatcg agattactgg accctactgt cgcccagtga tcgtccatca atgccacttt
     9601 gccgtggagc gggcggcggc ggtactcaaa cacctgaacg ccgcctttaa ccagatagtt
     9661 atacagtaaa cgtgcgccga ctctgacaat cggcatatcc ggttcgccct gaatgatcag
     9721 tttgatccgc accccgcgcc gtgccgcttt acgcaaggcg tgtaaaaaac gatagccggg
     9781 gaagaagtag gcgttggcga taatcacttc ccgccgcgcc tgagtgagca ttttcagata
     9841 gtgacgttcg atatcatcgc gatgttcttc gttatcgcgc cagaccagca atacctgcgc
     9901 ttctcccggc tggcggttct cttccgcttt atgatgacgt cgccaccagc gtcgcgccgc
     9961 gctctgtcct ggcaggtttt ccagctcaaa ctggagaata tcttcgacaa tcggcccttc
    10021 gaggcgaacc gcgtaatcct gtttggcctc cgggccgtag ctggacatat gctcggcgga
    10081 gtaattcagc ccgccaataa aggcgatacg cgcgtcgatc accacaattt tgcgatgcat
    10141 ccggcgaaac acattggtgc gcataccaaa aaggcgaggg cggggatcgt agtagcggaa
    10201 caccacgcca gctgccgtca gttcattgac aaactcatcg ctgagatccg gcgaaccgta
    10261 gccatccagc aagacttccg cttttacccc gcgttgcgct gctgccaata gcgccgcatg
    10321 cagctgtttg ccgacgtcat cctcaaacca gataaacgtt tcaagaatga tgcgttcttg
    10381 tgcctcgcca atcgccttaa acaccgcggg ataatattgc tcgccgtttt ccagcaactg
    10441 gatcttattg ccttcgcgcc agctacattt cataaatgaa tctccgcact taaaggggca
    10501 tgatcagaaa ggtgtcgcca tgtccgcagc ggcaacgcgg ttggcgcgct ggcgctggca
    10561 tttttgacgt agatcctgtc cagtcgtagt agaggaaatt gcaccggaaa cgtgcgcgcc
    10621 gggcgtccgt gggcgcgggt aaaaatctca tccagcccgg cctgcacttt taacggatga
    10681 ttagcttttt gccgccagtc gttgaaatca cccgctacca ataccggttc gccgtccggt
    10741 agctcattca cccattcggc gagcatcgca agctgcgcct gacggtgcgc ctcacgcagg
    10801 cccagatgta cacacatcac atgaatcgct ttcccggtca tcggcggcac aatgcggcag
    10861 tagagcacgc cgcgcttttc cgcaccatcg accgaaacat cacgattctc ataatgttca
    10921 atgggataac gtgaaagaac cgcattaccg tgatgtcctt ccgggtatac ggcattgcga
    10981 ccgtaggcga aatcgctcca catggtgtcg gcgagaaact cgtagtgcga ggtatcgggc
    11041 cagttttcca catgcagcgg atgaacttcg tgcgcgccca tcacttcctg caggcaaaca
    11101 atatcggcgc tgacggtacg cacggcgtcg cgaagttccg gcaaaatgaa gcgtcggtta
    11161 aacgcggtaa agcctttgtg aatattgatg gtgagcacct tgaacgaaaa ttgttgtgtt
    11221 tgatcgggca taattttcct gtctttgcct cttatctcat tgaaatagtg tagtcggcgt
    11281 cacaaaaagg tgcggtctta cggaattttc cgtaaagttc ggtactctga ataagtagag
    11341 ataaattctt caggagagaa gccatgaagt ggcaacaacg tgttcgtgtc gcaactggcc
    11401 taagttgctg gcagattatg ttgcatttac tggtagtggc gctgctggtg gtgggctgga
    11461 tgagtaaaac tctggttcac gtcggcgtag gattatgcgc actgtattgt gtcacggtag
    11521 tgatgatgct ggtgtttcag cgccaccccg agcaacgctg gcgtgaggtg gcagacgtgc
    11581 tggaagagct gaccacgacc tggtattttg gcgcagcgct gattgttctg tggctgttgt
    11641 cccgcgtact ggaaaacaac tttgtgctgg cgattgcagg gctggcaatc cttgccggcc
    11701 cggcggtagt gtctttgctg gcgaaagata agaagttaca tcaccttacg tctaaacatc
    11761 gcgtacgccg ctgaccctgt cgtggccgtt atcaccagta gcggccacaa actattccac
    11821 acaatatcca gactcgcatc cttcaaataa atctgcttgg taatgtccgt aaagtggcga
    11881 atagggttaa tccacgtcag attttgcagc cacactggca tgttttccac cggcgaaacg
    11941 taaccggaaa ggagaatggc gggcatcata aagacaaaca cgccgataaa cgcctgctgt
    12001 tgtgttgaac agagtgatga aatcaacaga ccgaatccca ccagtgataa accgtaaatc
    12061 accatcgtga agtagaacag cgccagcgat ccggcgaagg ggatttgata cgcccagata
    12121 ccaatcgcca gcacaatggt ggcctggaag gtggcgacaa ttaatgccgg tacggctttg
    12181 ccgatgaata tctgccaggt ggtgaggggc gaaaccagca actgatcgag cgtaccttgt
    12241 tcacgttcgc gggcgacgga aagtgaagtg acgctcatta cgccgatagt ggtgatcatg
    12301 gcgatcagcg acggcaccac aaaccatttg tagtcgagat tcgggttata ccagttgcgt
    12361 accaccagct cgctgttgtt gggtttcggt tttccttcca gcagctcctg ctgataattt
    12421 ttgacgatct gttgcaggta gttggcggca atttgcgcac tgttggagtt acgcccgtcg
    12481 aggatcaact gcaacggcgc ggtctggaag gtatccagtt tgcgcgagaa gtcagccggg
    12541 aaacgcacca gtagtaacgc tttttgtgtg tcgatggtcg ggcggatctc ctgcgggctt
    12601 ttcagcagca gcacatgggt aaaggcgctg gcgcgggcaa aacgttgagt aagttccacc
    12661 gaatgctcgc cgttatcttc atcgtagatg gcgatggtgg cgttggtcac ttccagcgtg
    12721 gcggcgaacg ggaacaggat cacctgaatc agcacgggta aaatcagaat tgcgcgggtt
    12781 tgcggttcgc gcagcagcga ctgcaactct ttgcggatta acgtccataa gcgatgaaac
    12841 atgctcttct ccctaatcca gccgacgttt ggttttcagc cacgtcaggc cgataaacat
    12901 caccgccgaa gcgatcaaaa acagcacgtt cactatcagt accaccggaa tattcccggc
    12961 gaggaacagg ctttgcaggg tgctgacgaa ataacgtgcg ggaataatgt acgtcaccgc
    13021 gcggatcacc gcgggcatac tgtctatctg aaatataaag ccggaaagca taatcgacgg
    13081 cagaaaagcc gcgttcaggg ccacctgagc ggcgttgaac tggttgcggg taatcgtgga
    13141 aatcagcagc cccatcccca gggtactgag taaaaacagg ctggagataa aaaacagaat
    13201 cagcagcgac ccgcgatacg gcacgccgag aataaacacc gacaccagca tacacagcaa
    13261 catcgccagc atcccgagaa agtaataagg gatcagctta cacagcagca gctccgtgcg
    13321 ggtaatctcc gtagagagca gagcctccat ggtgccgcgt tcccattctc gcgccaccac
    13381 cagcgaggtg agaatcgcgc cgatgaccgt catgataatg gtcaccgcac cggggataat
    13441 aaagtgctgg cttatcgctg ccgggttaaa ccagtagcgg gtttgtacat caataagcgg
    13501 ttcaaaagtc tgcccgttat cctccgcgcg ctgcatttgc cagatctgcc agatcccttc
    13561 gacatacccc tgcacaaagt tagcggtatt cggctcactg ccgtcggtga tcacctgaat
    13621 cggtgcggtg gcgttggcgc gctccatctg ttcggcgaaa tccaccggaa taaccaccag
    13681 accgcgaatt ttccccgcct gcattttggc gatcagttcc tgacggttat cgctgatggt
    13741 ggcgtcgatg tagggcgaac cggtcatggt gtgggtgaaa tccagcgccg cttcgctacg
    13801 ctgttccagt aaaatcccga cgcgcagctt gctggagtcg aggttaatgc cgtatccaaa
    13861 aataaacagc agtagcagtg ggatcactac cgcaatcagc cagctactcg gatcgcgaac
    13921 gatctgccgc gtctctttga cgcacagcgc ccgcacgcga cgccaggaca ggatcggatt
    13981 actcattgct atgctcctta tcccagtcgt ggatcaactg aataaaggct tgctccatgg
    14041 tgggatcggg ttgctcatcg ttggccgact gcgctttcaa atcgtccggt gtgccgctgg
    14101 cgattaattt cccgcggtac accaggccga tgcggtcgca atattccgct tcatccataa
    14161 agtgggtggt gaccatcacc gtgacgcctt tctctaccat actgttgata tgtagccaaa
    14221 attcacggcg ggtgagggga tcaacgccgg acgtcggttc gtcgagaaac agaatgtccg
    14281 gttcatgcat cagcgaacag gccagcgcca gccgctgttt aaaacctaat ggcagttcat
    14341 cggtcgcgtg ggaggcgata cttttcaggc cgaacgcctc gctcatacgg gagatttttt
    14401 cgttctgcgc ccgaccgcgt aagccataca caccagagaa aaagcgtaaa ttctgttcga
    14461 ccgtcaggtt gccgtagagc gaaaattttt gcgccatata gccgagatgc tggcgcgctt
    14521 taccggaact ctctttcaga tccatgccca gcaccagcgc ctgaccagag gtcggtacca
    14581 gcaaaccgca catcatctta aaggtggtcg atttacccgc gccgtttggt ccgagcaaac
    14641 caaaaatctc cccgcgttta acggcaaagt tgacgtgatc ggtggcggca aaatcaccaa
    14701 atttcttggt tagttccttc gcttcgatca ccgtctcgcc gggtgtgcct tctaccgtat
    14761 gtaatattgc gcccagcggc gattccgagg ttccggcacc gcccagcaaa tcaataaacg
    14821 catcttcaaa acgcggcgta gtttcgttga tgtcgatttc cggcatcccg tcggcatggc
    14881 gaatatcgtc tggcgtggtc tcttttttga ggatcagacg taccgatttc ccctgaatca
    14941 tgccgtcgct gacctgcggc agtttcaatg cccgttgcaa cagtttgcgg ttgccctcgt
    15001 gcgggctggt catcagaaag ctgcgtccgg ccatagtttg agtcagggcc gtcggttctc
    15061 cctgatacag cagctcgcct tcgttcatca gtaacacgtc acggcactgc tcggcttcgt
    15121 cgagatacga ggtactccag aggattaaca tcccttcgcc cgccagctca tgcaccatct
    15181 gccacagttc gcgccgtgag atagggtcaa cgccgacgcc gggttcatcg agcagcaaca
    15241 ctttcggttc gcctaccagg gtacaggcca ggccaagttt ttgtttcatc ccaccggaga
    15301 gcttgcccgc caggcgtccg gtaaacgggc caagagacgt aaactccagc aggcgagcaa
    15361 aagtttgctt ccgtgcctcg ccggtgacgc tgcgcaaatc cgcgtacaga ttgaggttct
    15421 ccatcaccgt cagatcttca tacagaccaa atttctgcgg catatagccg agcacagcgt
    15481 gcagcgcagc gtcatttttg atcggatcaa agccaatcac cgtggcactg ccgctgtcgg
    15541 gtttcagtaa tcccgccagc atccgcatca gcgtggtttt gcccgcaccg tccggcccaa
    15601 ccagccccgt cacataaccg gcgtgaatgg tgcagtcgag cggtgcgacg gcgggcttgt
    15661 ccatgcccgg aaagcgtttt tccaggccgt tcagcgtgat aacggcatca ttcatgtcct
    15721 gcctcgtcac cgaattgcac cgttaccggc attccctggc gtaacgcatc atcggcgtcg
    15781 gtcaccacaa tacgcaggcg atagacgagg tcggtacgca gatccggtgt ttcgacggtt
    15841 ttcggggtaa attcagcagt cggcgaaacg aaaccaatct gcccgtgata cggtttgttc
    15901 ggacgaccat cggtataaag cagcactttg cgccccggct gggcctggtc aagattacgt
    15961 tcatcaacat aggcgcgtac ccacaccgga cgcgtcagcg agacggtaaa taccgtgcca
    16021 ccttcattaa gcaccgtgcc tggctctacc gcgcgcgtta acagcgtgcc atcagacgga
    16081 gcgaccagcg tcgaatcctg caaattcagc tccgcctgcg ccagttgcgc ctgcgcttgt
    16141 tcgaggctgg ctttcgcctg ggcgatgtcc tgttcgcggt taccggaacg gtactgacgc
    16201 aatttatcct gcgctgattt cagcgtggcc tgcgcctggt cgcgcgagga gcgggcattt
    16261 tccaggtcat ttgccgaaat ggtgcggctt ttccacagtc cttgctggcg gttatagaag
    16321 ttctgcgcat agtcataggc agcttgcgcc tgtttcaccg ctgcggcggc ctgagcgatt
    16381 tcttcatctc gatacccggc aagcatcagg tcatactgcg cctgagccac cgaaacgccc
    16441 gctttcgcct gcatcagggc aatctcatac ggcttgtgat ccagttctcc cagcacctgg
    16501 cccgctttga tcgcatcacc ctcgtccacc gccagcgact caacgcgccc cccaacacgg
    16561 aaactaagat tcaccgtacg aatatccacg ttgccataca gcgtcaggcc gttatcctgg
    16621 cggctttgat accaccagta gcctccggca accacggcgg caagtactac taccgccagt
    16681 ccgatcacga caggtttttt catcactaca aactcctttg cgataatcct tgcaaaatca
    16741 ggtcaatatg acaggtcacc gtctggttga tcagttcggt cttttcttca tcgaacgcgg
    16801 tccagccggt acgtaacaga atagtttctt tgccaagacg aaacgccaga atctcaccaa
    16861 tcaacgcatg ggtatgaagg atcatgcggg tatcgctagc gtcgcagccg gtccaggcgg
    16921 caatcaggcg tgtaaggtga ctgtgtagcg gactaatcac ctgttcgtgc accaggtggt
    16981 aggctgccgt gggagagagc tgctcacggg agataaattt gctgaggttg acggtgtcat
    17041 cctgggtgag cagcttaatc atgtttctgc aggcgcgaag gatcagttca cggatggcag
    17101 cccgatccgg ctgtggttgc gcgaacaaac gttcggcttc ttcggcgtgt ggacggaact
    17161 gctcgccaat aaaatcggca atccactggg cgcaggcgag gtacaaatct tctttcgaac
    17221 cgaagtagta ggtgatggct gcgatattct gcccggcctg ggcggctatc tcgcgggtgg
    17281 tggcgttcat tccatattca ccaaactgtg ccagtgccgc agcaatcagc tgttttttcg
    17341 cctgttcacc cttgattgtc atggcagaat tattcatcac acagtccatt cttaatcaaa
    17401 tgattgatta agattatgac tccatagggg agttgtccag tatggctaag aattttagct
    17461 acgccagtca cagggataat ttatgcgctg cgtcacaaaa actgctacac tccgctccct
    17521 catgacattg tggtttttgt cattttcctt ttcagtatct ccctgaaaac tacaccggta
    17581 acggtcgggg cggttcggag tagttatgtc tttcgattct ttgggtttaa gccctgatat
    17641 cctgcgcgcc gttgccgagc agggttaccg tgaacccacc cctattcagc agcaggcgat
    17701 ccctgcggtg ctggaaggcc gcgacctgat ggccagcgcc cagaccggta ccggtaaaac
    17761 agcgggcttt acgctgccgc tgttgcaaca cctgatcact cgccagccgc acgccaaagg
    17821 gcgtcgtccg gtgcgtgcgc tcattcttac cccgacccgt gaactggcgg cgcagattgg
    17881 cgaaaacgtc cgtgattaca gcaaatacct gaacattcgt tcgctggtgg tgtttggcgg
    17941 tgtcagcatt aacccgcaga tgatgaagct gcgcggcggc gttgatgtgc tggtggcaac
    18001 gccgggacgt ctgctggacc tggaacatca aaatgcagtg aagctggatc aggttgaaat
    18061 cctcgtcctt gatgaagctg accgcatgct cgacatgggc tttatccacg atatccgtcg
    18121 cgtgttaaca aaactgccaa cgaagcgcca gaacctgttg ttctccgcga ccttctctga
    18181 cgatattaaa gccctggcgg aaaaactgtt gcacaacccg ctggaaatcg aagtggcacg
    18241 ccgcaatact gcgtctgatc aggtgaccca gcacgttcac tttgtcgata agaaacgcaa
    18301 acgcgaattg ctgtcgcata tgattgggaa agggaactgg cagcaggtgc tggtgtttac
    18361 ccgtaccaaa catggcgcta accatctggc tgaacagctt aataaagacg gcatccgtag
    18421 tgcggcgatc cacggtaata aatcgcaagg tgcgcgtact cgtgcgctgg ccgattttaa
    18481 atcgggcgat attcgtgtac tggtggcaac tgacatcgct gcgcgcggtc tggatatcga
    18541 agagttgccg cacgtcgtca actatgagtt accaaacgta ccggaagatt atgttcaccg
    18601 tatcgggcgt actggccgtg cggcggcaac tggcgaagca ttgtcgctgg tgtgtgttga
    18661 tgaacacaaa ctgctgcgtg atatcgaaaa actgctgaaa aaagagatcc cgcgcattgc
    18721 gattccgggg tatgagccgg acccgtcaat caaagccgaa ccgatccaga acggtcgcca
    18781 gcaacgtggc ggcggtcgtg gacagggcgg tggtcgcggt caacagcaat cacgccgtgc
    18841 ggaaagtggt gcgaagtctg gtaacgcgaa gcccgcagaa aaaccgtctc gtcgtctcgg
    18901 cgatgccaaa ccggcaggcg aacaacaacg tcgccgccgt ccgcgtaaac ctgccgctgc
    18961 gcagtaatct tttatgccgg gctatgcccg gcatgagact gatgacaaac acaaaactgc
    19021 ctgatgcgct acgcttatca ggcctacgtg aactctgcaa tatattgaat gtgcatgttt
    19081 ttgtaggccg gataaggcgc tcgcgccgca tccggcattt cacaacaagc acttgtcagc
    19141 catttgcgag cgcggcaaaa agatttctct gttttaacgc ccacaagcca cccgatgcgt
    19201 tacttctccg cagccaattg tccgcgcaac accattaaaa ggtaccccag gcgattcttg
    19261 ccctgaccat taccgccatc ttcccagtaa gcatcatttt gcgtatgctc aaccagtttt
    19321 gcggacgcgg ttgccagcaa gagtgtacgc agttctgcat gttgttcgaa tttagcgcga
    19381 agcgctttac acatcatcct gctggttatt gcgagccgct ttccagaaac agaaaaacca
    19441 ttacccctga aaaccgaaaa atgccacaat attggctgtt tatacagtat ttcaggtttt
    19501 ctcatggcat taaccgccgc gcttaaagcg caaattgccg cctggtataa ggcgcttcag
    19561 gaacagatcc ccgactttat tccccgtgcg ccgcagcggc agatgattgc ggacgtcgcc
    19621 aaaacgctgg ccggagaaga agggcggcat ctggcgattg aagcccctac cggcgttggg
    19681 aaaacgctct cctatttgat ccccggcatc gccattgccc gcgaagagca aaaaacgctg
    19741 gtggtaagta ccgccaacgt ggcattgcag gatcagatct acagcaaaga tttaccgctg
    19801 ctgaaaaaga tcatttccga tcttaaattc actgccgctt ttgggcgtgg gcgctacgtt
    19861 tgcccgcgta atctgacagc gctcgccagc actgagccca cgcaacagga tctgctggcg
    19921 tttcttgacg acgaactgac gccgaacaat caggaagagc aaaaacgttg tgcgaagctg
    19981 aagggcgatc tcgacactta taaatgggat ggtctgcgcg atcatactga tatcgctata
    20041 gatgatgatc tctggcgtcg tttgagtacc gacaaagcca gctgcctcaa ccgcaactgt
    20101 tattactatc gcgaatgccc gttttttgtc gctcgtcggg aaattcagga agcggaagtg
    20161 gtggtggcaa accatgcgct ggtgatggcg gcgatggaaa gcgaagccgt attgcctgac
    20221 ccgaaaaatt tactgctggt gctggacgaa ggtcatcacc tgccggatgt ggcgcgggat
    20281 gcgctggaga tgagcgccga aatcactgcg ccctggtacc ggctccaact ggacctgttc
    20341 accaaactgg tcgccacctg catggagcag tttcgcccga agaccatccc accgctggcg
    20401 atccctgaac gtttgaatgc gcattgtgaa gagctgtatg agcttatcgc ctcgttaaac
    20461 aacattctca atctctacat gcctgccggg caggaggcag agcaccgttt tgcgatgggc
    20521 gaactgcctg atgaagtgct ggagatctgc cagcggctgg caaaactcac cgagatgctg
    20581 cgtggtctgg cggagttatt tcttaacgat ttaagtgaga aaaccggcag ccatgacatt
    20641 gtacgcctgc atcggttgat tttgcagatg aaccgcgcgt tggggatgtt cgaggcgcaa
    20701 agcaaactct ggcggctggc gtctctggcg caatcttccg gtgcgccggt gaccaaatgg
    20761 gcgacgcggg aagagcgcga agggcagcta cacctttggt ttcactgcgt gggaatacgt
    20821 gtcagcgatc agctggaaag gctgctgtgg cgcagtattc cgcacattat tgttacctcc
    20881 gcaaccttgc gttcgctgaa cagtttttcg cgtttgcagg agatgagtgg gctgaaagag
    20941 aaagcgggtg accgctttgt ggcgctggat tcgcccttta accactgtga acagggcaaa
    21001 attgttattc cccggatgcg cgttgagcct tccatcgaca acgaagagca gcacattgct
    21061 gaaatggcgg cctttttccg tgagcaggtg gagagcaaaa aacatctcgg tatgttggtg
    21121 ctgtttgcca gcgggcgtgc gatgcagcgc tttctcgact atgtgacgga tttacgcctg
    21181 atgttgctgg tgcagggcga tcagccacgt tatcgcttag ttgaactgca ccgcaaacgc
    21241 gttgccaacg gtgagcgtag cgtgctggtg ggtttacagt cctttgccga aggtcttgat
    21301 ctgaaaggcg atctgctcag ccaggtgcat atccacaaga tcgcttttcc gcccatcgac
    21361 agtccggtgg tgatcaccga aggtgaatgg ctgaaaagcc tcaaccgcta tccgtttgag
    21421 gtgcaaagcc tgccgagcgc ttcgtttaac ctgattcagc aggttgggcg actgattcga
    21481 agtcacggtt gctgggggga agtggttatc tacgacaaac gcttgctgac aaaaaattat
    21541 ggcaagcgac tactggatgc attaccggta tttccgatag agcaaccgga agtccctgaa
    21601 ggtatagtta aaaagaaaga aaaaacgaaa tccccacgcc gtcggcggcg ttaatgatgt
    21661 gagtcaggta aggagtcgta aatggactat cgcaaaatca ttaaagagat cgggcgcggg
    21721 aaaaaccacg cgcgcgattt agaccgggat actgcgcgcg gtctgtatgc ccatatgctc
    21781 aatggtgaag tccctgacct tgagttgggc ggcgtattga ttgcgctacg tatcaaaggg
    21841 gagggggaag cagagatgct cggcttttac gaagccatgc aaaatcacac catcaagctg
    21901 acgccgcctg caggcaagcc gatgccgatt gtcatcccca gttacaacgg cgcgcgtaaa
    21961 caggccaacc tgacgccgtt gctggcgatt ctgctgcaca aactcggttt tcctgtggtg
    22021 gttcacgggg ttagcgaaga tccaacccgc gtgctgaccg aaaccatttt cgaactgatg
    22081 ggtattacgc caacgttgca cggcgggcag gcgcaggcga agctcgacga acatcaacct
    22141 gtctttatgc ctgtcggcgc gttttgcccg ccgctggaaa aacaactggc gatgcgctgg
    22201 cggatgggcg tgcgtaacag tgcacatacc ctggcgaaac tggcgacgcc atttgccgaa
    22261 ggtgaggcac tgcgtctttc cagcgtttcg catccggaat acattggacg cgtcgcgaag
    22321 ttctttagtt atatcggcgg gcgggcgcta ctaatgcatg gtactgaagg tgaagtgtat
    22381 gctaatccgc agcgctgccc gcagatcaat ctcattgacc gtgaagggat gcgggtactg
    22441 tatgaaaaac aggacactgc tggtagcgag ttactgccac aagcaaaaga tccggaaacc
    22501 acggcgcagt ggattgagcg ttgccttgct ggcagcgaac cgattcccga atcactgaaa
    22561 atccagatgg cttgctgcct gatggctacg ggtgaagcgg caactatcag cgacggcctg
    22621 gcgcgcgtta atcaggcatt ttaattttta tctcagccgg atgagccatg tcatccggct
    22681 tttaccccac cgcaatatga aattcctgca tctttattga ccttcccgcg cccggcgtgc
    22741 agcataaaaa aacaacaaac acataacata aacagcaact atgtttaacc tcattggttg
    22801 tttttttgaa caataatatg agtcaagtaa tttcatcatt gtggtgctgt atatggtgcc
    22861 tgattttatt tagcaactct tctaagtttc ttgatcttag caaggtaata ccagtgaact
    22921 taaagatcat gtttactttt tgatctctat caactctatc aggaagatcg tgtgaatagt
    22981 catcaagttc aattgcacat atgattgaaa aatcattttt attaacaatc aaaaaatcta
    23041 tatgatattg agatatagac ctaaatgctt ttataaatga attatattga gtgttcttgt
    23101 caaatatagg aacgataatg tctaccattc taacctgtga aaacacaaaa aagtcctgat
    23161 gaaattcatc ataaatcaat ttaaaaaagc gacgttcgcg attgctcatg aaatttttct
    23221 tttgatagaa ctggaatgca tcatactgaa gtgtatgaga tttctttgat gttgctttat
    23281 aattaaagag aggaggggca taatttggtg tgttatctgg agcatggtgt aacgtcttat
    23341 gtatactttc tttttttagt tttttatctg tgagtcctcg tattaatgaa tataatgcca
    23401 aaaataaaag atagaaaaaa aatagagcta taactgaaag taacagggca agaggtattt
    23461 gccataaggg gattttggga aacataaaac gatcctacaa atgaaagggg cgatattata
    23521 tggtaaaaaa acgaagatta atacaatgaa tcggtaagat tttaaaagat atcaacgtta
    23581 atacactgta tttagatata gtattatgaa cagtttatat aaaaggatta taagaaggtc
    23641 gcgacaggaa aatatcctaa ggcgacctta gagtgaggag acagacatca tttgatgtga
    23701 ttatccggct taaca
//
LOCUS       DAZFJL010000075        23652 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFJL010000075 DAZFJL010000000
VERSION     DAZFJL010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.075"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            3..704
                     /gene="bcsQ"
                     /locus_tag="ACHJ8C_002803"
     CDS             3..704
                     /gene="bcsQ"
                     /locus_tag="ACHJ8C_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO5552636.1"
                     /translation="MTAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWARA
                     MLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICSGLQQLKASGRYQ
                     WILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINDFRIGS
                     QVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEFRSDALAAEEILTLANWC
                     LLNYSGLKTPVGSAS"
     gene            701..3319
                     /gene="bcsA"
                     /locus_tag="ACHJ8C_002804"
     CDS             701..3319
                     /gene="bcsA"
                     /locus_tag="ACHJ8C_002804"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO5552637.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLFHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIIL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPAQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            3330..5669
                     /gene="bcsB"
                     /locus_tag="ACHJ8C_002805"
     CDS             3330..5669
                     /gene="bcsB"
                     /locus_tag="ACHJ8C_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO5552638.1"
                     /translation="MKRKIFWICAVALGMSAFPSFMTQATPATQPLINAEPAVAAQAE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVELQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIVGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            5676..6782
                     /gene="bcsZ"
                     /locus_tag="ACHJ8C_002806"
     CDS             5676..6782
                     /gene="bcsZ"
                     /locus_tag="ACHJ8C_002806"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO5552639.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNLLDWTQNNLAQGSLKEHLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            6764..10237
                     /gene="bcsC"
                     /locus_tag="ACHJ8C_002807"
     CDS             6764..10237
                     /gene="bcsC"
                     /locus_tag="ACHJ8C_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO5552640.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVIAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTSDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HNSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAYIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPHAQWNSNIQE
                     LVNRLQNDQVLETANRLRENGKEAEAEAMLRQQPPSSRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPTQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            10319..12307
                     /gene="hmsP"
                     /locus_tag="ACHJ8C_002808"
     CDS             10319..12307
                     /gene="hmsP"
                     /locus_tag="ACHJ8C_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO5552641.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPPQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQQRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQESGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12490..13776
                     /gene="dctA"
                     /locus_tag="ACHJ8C_002809"
     CDS             12490..13776
                     /gene="dctA"
                     /locus_tag="ACHJ8C_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO5552642.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            13996..15492
                     /locus_tag="ACHJ8C_002810"
     CDS             13996..15492
                     /locus_tag="ACHJ8C_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO5552643.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPVQ"
     gene            complement(15588..16517)
                     /gene="kdgK"
                     /locus_tag="ACHJ8C_002811"
     CDS             complement(15588..16517)
                     /gene="kdgK"
                     /locus_tag="ACHJ8C_002811"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO5552644.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWRGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16749..17516
                     /gene="pdeH"
                     /locus_tag="ACHJ8C_002812"
     CDS             16749..17516
                     /gene="pdeH"
                     /locus_tag="ACHJ8C_002812"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO5552645.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIEDLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            17586..19646
                     /locus_tag="ACHJ8C_002813"
     CDS             17586..19646
                     /locus_tag="ACHJ8C_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO5552646.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FQLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLIYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(19687..21009)
                     /locus_tag="ACHJ8C_002814"
     CDS             complement(19687..21009)
                     /locus_tag="ACHJ8C_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5552647.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFAVMVPLAGLLADAFGRRKSMVVITTLIILFALF
                     AFNPLLGSGNPALVFVFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21402..22415)
                     /gene="yhjD"
                     /locus_tag="ACHJ8C_002815"
     CDS             complement(21402..22415)
                     /gene="yhjD"
                     /locus_tag="ACHJ8C_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO5552648.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22464..23363)
                     /locus_tag="ACHJ8C_002816"
     CDS             complement(22464..23363)
                     /locus_tag="ACHJ8C_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5552649.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARSLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGIAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
BASE COUNT         5524 a         6065 c         6606 g         5457 t
ORIGIN      
        1 ccatcaccgc cgcattagcc tggtcattac aaatgttggg agaaaatgtc ctggtggtcg
       61 atgcctgccc ggacaacttg ttgcgcctgt catttaacgt tgattttacc caccgtcagg
      121 gttgggccag agctatgctg gatggccagg actggcgtga cgccgggttg cgctatacct
      181 cgcagctcga tttgctgcct tttggtcagt tatccattga agaacaagaa aatccacagc
      241 actggcaaac ccggctgagc gatatttgct ccggcttaca gcaactaaaa gccagcgggc
      301 gttaccagtg gattttaatt gacttaccgc gtgatgcctc gcagataacc caccagctgc
      361 tgagtttgtg cgatcactcg ctggcaatcg tcaatgtgga tgccaactgc catatccgac
      421 tgcatcagca agcgctgccg gatggtgcac atattttgat taatgacttc cgtattggca
      481 gccaggttca ggacgatatt taccagcttt ggttgcaaag ccagcgccga ttgctgccga
      541 tgctcattca tcgtgatgaa gcgatggctg aatgtctggc agctaagcaa ccagtcggtg
      601 aatttcgcag tgatgcgctg gcggctgaag aaatactgac gctggctaac tggtgcctgt
      661 taaactattc cgggctgaaa acgccagtcg ggagtgcatc atgagtatcc tgacccggtg
      721 gttgcttatc ccgccggtca acgcacggct tattgggcgt tatcgcgatt atcgtcgtca
      781 cggcgcgtcg gctttcagcg cgacgctcgg ctgtttctgg atgatcctgg cctggatttt
      841 tattccgctg gagcacccgc gctggcagcg tattcgcgca gaacataaaa acctgtatcc
      901 gcatatcaac gcctcgcgtc cgcgtccgct ggacccggtc cgttatctta ttcaaacatg
      961 ctggttactg atcggtgcat cgcgcaaaga aacgccgaaa ccgcgcaggc gggcattttc
     1021 aggtctacaa aatattcgtg gacgttacca tcaatggatg aacgagctgc ctgagcgcgt
     1081 tagccataaa acacagcatc ttgatgagaa aaaagagctc ggtcatttga gtgctggggc
     1141 gcggcggttg atccttggta tcatcgtcac cttctcgctg attctggcgt taatctgcgt
     1201 tactcagccg tttaacccgc tagcgcagtt tatcttcctg atgctgctgt ggggggtagc
     1261 gctgatcgta cggcggatgc cggggcgctt ctcggcgctg atgctgattg tgctgtcgct
     1321 gaccgtttct tgccgttata tctggtggcg ttacacctcg acgttaaact gggacgatcc
     1381 ggtcagcctg gtgtgcgggc ttattctgct gttcgccgaa acgtacgcgt ggattgtgct
     1441 ggtgctcggc tatttccagg tcgtatggcc tctgaatcgt cagccggtgc cattgccgaa
     1501 agatatgtcg ctgtggccgt cggtggatat ctttgtcccg acttacaacg aagatctcaa
     1561 cgtggtgaaa aataccattt acgcttcgct gggtatcgac tggccgaaag acaagctgaa
     1621 catctggatc ctcgatgacg gcggcaggga agagtttcgc cagtttgcgc aaaacgtggg
     1681 ggtgaaatat atcgcccgta ccactcatga acatgcgaaa gcgggcaaca tcaacaatgc
     1741 gctgaaatat gccaaaggcg agttcgtgtc gatattcgac tgcgaccacg taccaacgcg
     1801 atcgttcctg caaatgacca tgggatggtt cctgaaagag aaacagctgg cgatgatgca
     1861 gacgccgcac cacttcttct cgccggatcc gtttgaacgc aacctggggc gtttccgtaa
     1921 aacaccgaac gaaggcacgc tgttctatgg tctggtgcag gacggcaatg atatgtggga
     1981 tgccactttc ttctgcggct catgtgcggt gattcgccgt aagccgctgg atgagattgg
     2041 cggtattgct gtcgaaacgg taaccgaaga tgcgcacact tctctgcgtc tgcaccgtcg
     2101 tggctacacc tccgcgtata tgcgtattcc gcaggcagcg gggctggcga ccgaaagtct
     2161 ctcggcgcat atcggtcagc gtattcgctg ggcgcgcggg atggtgcaaa tcttccgtct
     2221 cgataacccg ctcaccggta aagggctgaa gtttgctcag cgactgtgct atgtcaacgc
     2281 catgttccac ttcttgtcgg gcattccacg gctgatcttc ctgactgcgc cgctggcgtt
     2341 cctgctgttt catgcctaca tcatctacgc gccagcgttg atgatcgccc tgtttgtgct
     2401 accgcatatg atccatgcca gcctgactaa ctcgaagatc cagggcaaat atcgccactc
     2461 tttctggagt gaaatctacg aaacagtgct ggcgtggtat atcgcaccac cgacgctggt
     2521 ggcgctgatt aacccgcaca aaggcaaatt taacgtcacc gccaaaggtg gactggtgga
     2581 agaagagtac gtcgactggg tgatctcgcg gccctacatc ttccttgttc tgctcaacct
     2641 ggtgggcgta gcggttggga tctggcgcta cttctatggt ccgccaaccg agatgctcac
     2701 cgtggtcgtc agtatggtgt gggtattcta caacctgatt attctcggcg gcgcagtcgc
     2761 agtatcggta gaaagcaaac aggtacgccg atcgcaccgc gtggagatga cgatgcccgc
     2821 agcaattgcc cgcgaagatg gtcacctctt ctcgtgtacc gttcaggatt tctccgacgg
     2881 tggtttgggg atcaagatca acggtcaggc gcagattctg gaagggcaga aagtgaatct
     2941 gttgcttaaa cgcggtcagc aggaatacgt cttcccggct caggtggcgc gcgtgatggg
     3001 taatgaagtt gggctgaaat taatgccgct cactacccag caacatatcg attttgtgca
     3061 gtgtacgttt gcccgtgcgg atacatgggc gctctggcag gacagctacc cggaagataa
     3121 gccgctggaa agtctgctgg atattctgaa gctcggcttc cgtggctacc gccatctggc
     3181 ggaatttgcg ccttcttcgg tgaagggcat attccgtgtg ctgacttctc tggtttcctg
     3241 ggttgtatcg tttattccgc gccgcccgga gcggagcgaa acggcacaac catcggatca
     3301 ggctttggct caacaatgat gataacgcga tgaaaagaaa aatattctgg atttgtgcag
     3361 tggctctggg gatgagtgcg ttcccctctt tcatgacgca ggcgacgcca gcaacgcaac
     3421 cactgatcaa tgctgagcca gctgtagccg cccaggcgga gcaaaatccg caggtggggc
     3481 aagtgatgcc gggcgtgcag ggcgcagatg cgccagtcgt ggcgcagaac ggtccttccc
     3541 gtgatgtgaa gctgaccttt gcgcaaattg cgccgccgcc gggcagcatg gtgctacgtg
     3601 gcattaaccc gaacggcagc attgagtttg gtatgcgcag cgatgaagtg gtgacgaagg
     3661 cgatgctcaa cctcgaatac acgccatcgc catcattact gcctgtccag tcgcagttaa
     3721 aggtttatct caatgatgaa ctgatgggcg tgctgccagt gaccaaagaa cagttgggta
     3781 aaaaaacgct ggcacaaatg cccattaacc cgctgtttat tactgatttc aaccgtgtgc
     3841 ggctggagtt tgtcggccat tatcaggacg tgtgcgaaaa cccggccagc accacgcttt
     3901 ggctggatgt tgggcggagc agtggactgg atctgaccta tcagaccctg aatgtgaaga
     3961 atgacctgtc acacttcccg gtgccattct ttgacccgcg tgataaccgc accaacacct
     4021 tgccgatggt ctttgcgggt gcgccggatg ttgagctgca acaagcatct gccattgtcg
     4081 cctcgtggtt tggttcgcgt tctggctggc gtgggcagaa cttcccggtg ctctataacc
     4141 aactgccgga tcgcaatgcc attgtctttg ccaccaatga caaacgcccg gacttcctgc
     4201 gcgatcatcc ggcggtaaaa gccccggtga ttgagatgat taaccatccg cagaatcctt
     4261 acgtcaaact gctggtggtg tttggtcgtg acgacaaaga cttgttgcag gcagcgaaag
     4321 gtatcgctca gggtaacatt ctgttccgtg gtgaaagtgt ggtagtgaat gaagtgaaac
     4381 cgctgctacc gcgtaaaccg tacgatgcgc cgaactgggt gcgtaccgat cgtccggtca
     4441 cctttggtga actgaaaacc tatgaagaac aattacaatc cagcggtctt gagccagcag
     4501 cgattaacgt ttcgctaaac ctgccgccgg atctctacct gatgcgcagt accggcattg
     4561 atatggatat taattaccgc tacaccatgc cgccggtgaa agacagttcg cggatggata
     4621 tcagcctgaa taaccagttc ctgcaatcct tcaacctgag cagcaaacag gaggcgaacc
     4681 gcctgctgct gcggattccg gtattacaag gtttgctgga tggcaaaacg gatgtctcta
     4741 ttccagcgct gaaactgggc gcgaccaacc agctgcggtt cgactttgag tatatgaacc
     4801 cgatgccggg cggttcggtg gataactgca ttaccttcca gccggtgcag aatcatgtgg
     4861 tgattggtga cgactccacc atcgacttct cgaagtatta ccacttcatc ccgatgccgg
     4921 atctacgcgc ctttgctaac gcgggcttcc cgttcagccg gatggcggat ttgtcgcaaa
     4981 ccatcaccgt gatgccgaaa acgccaaacg aagcacagat ggaaaccttg ctgaataccg
     5041 ttggctttat cggtgcgcag acgggcttcc cggcgattaa cctgacggtg accgatgatg
     5101 gcagcaccat tcagggcaaa gatgccgaca tcatgatcgt cggtggtatc ccggacaaac
     5161 tgaaagatga caagcagatc gacctgttgg tgcaggcgac cgaaagctgg gtaaaaacgc
     5221 cgatgcgcca gaccccgttc cccggtattg tgccggacga gagcgatcgc gcggcagaaa
     5281 cccagtcaac gctgacctct tccggtgcga tggcggcggt gattggcttc cagtcgccgt
     5341 ataacgacca gcgcagcgtg attgcgctgc tggcagatag cccacgcggt tatgaaatgc
     5401 ttaacgatgc ggtgaacgat agcggcaaac gcgccaccat gttcggttcg gtcgcggtga
     5461 tccgcgagtc cggtatcaac agcctacgtg ttggcgatgt ttattacgta ggtcatctgc
     5521 cgtggttcga gcgcttgtgg tatgcgctgg caaaccatcc gattctgctg gcggtgctgg
     5581 cggctatcag tgtgatattg ctggcgtggg tactgtggcg tctgctgcgt attattagtc
     5641 gtcgtcgtct taacccggat aacgagtaat tgaagatgaa tgtgttgcgt agtggactcg
     5701 tgacgatgct gctgctggct gcctttagtg ttcaggcagc ctgtacctgg cctgcctggg
     5761 agcagtttaa aaaggattac atcagtcagg aagggcgcgt catcgacccc agcgacgcgc
     5821 gcaaaatcac cacctccgaa gggcaaagtt acggcatgtt ctttgccctg gcggctaacg
     5881 accgtgcagc tttcgataat cttctcgact ggacgcagaa caatctcgct cagggttctt
     5941 taaaagaaca tttgcccgcc tggctgtggg gcaagaaaga gaacagtaag tgggaagtgc
     6001 tggacagcaa ttcggcctcc gatggtgatg tctggatggc ctggtcgttg ctggaggcgg
     6061 ggcgtttgtg gaaagagcag cgttataccg acatcggcag cgcattgcta aaacgtatcg
     6121 cgcgggagga agtggtgacg gtgcctgggc tgggctccat gttgttaccg ggcaaagtgg
     6181 gttttgctga ggataacagc tggcgtttta accccagcta cctgccgccg acgctggcgc
     6241 agtatttcac ccgctttggc gcgccgtgga ccacgctgcg cgaaaccaat caacgtttat
     6301 tgctggaaac cgccccgaaa ggcttttcgc cagactgggt gcgctatgag aaagacaaag
     6361 gctggcagct aaaagccgaa aaaacattga tcagcagcta cgacgctatc cgcgtttaca
     6421 tgtgggtagg catgatgcct gacagcgatc cgcaaaaagc gcggatgctc aaccggttta
     6481 aaccgatggc gacattcact gagaaaaacg gttatccgcc ggaaaaagtg gatgtggcta
     6541 cggggaaagc gcagggtaaa ggaccggtcg gtttttctgc cgccatgctg ccctttttac
     6601 aaaaccgcga tgcgcaggcc gttcagcgcc agcgcgtggc cgataacttt cccggcagcg
     6661 atgcctatta caactatgtg ctgaccctgt ttggacaagg ctgggatcaa caccgtttcc
     6721 gcttctcgac aaaaggtgag ttattacctg actggggcca ggaatgcgca aattcacact
     6781 aaacatattc acgctttccc tcggtctggc cgtcatgccg atggtcgagg cagcaccaac
     6841 cgctcagcaa cagttgctgg agcaagttcg gttaggcgaa gcgacccatc gtgaagacct
     6901 ggtgcaacag tcgttgtatc ggctggaact tattgatccg aataacccgg atgtgattgc
     6961 tgcccgtttt cgttctctgt tacgtcaggg cgatattgat ggcgcacaaa aacagctcga
     7021 tcggctgtcg cagttagcgc cgagttcaaa tgcgtataaa tcgtcacgga ctacgatgct
     7081 gctttccacg tcggatggtc gtcaggcact gcaacaggca agattgcagg cgacgactgg
     7141 tcatgcggaa gaagctgtgg cgagttacaa caaactgttc aacggtgcgc cgccggaagg
     7201 tgacattgct gtcgagtact ggagtacggt ggcgaaaatt ccggcccgcc gtggcgaagc
     7261 gattaatcag ctaaaacgca ttaatgcgga tacgccgggc aacacgggcc tgcaaaacaa
     7321 tctggcgcta ttactgttta gtagcgatcg ccgtgacgaa ggttttgccg tcctggaaca
     7381 gatggcgaaa tcgaacgccg ggcgcgaagg ggcctctaaa atctggtacg ggcagattaa
     7441 agacatgccc gtcagcgatg ccagcgtgca ggcactgaaa aaatatctct cgatctttag
     7501 cgatggcgat agcgtagctg ctgcacaatc gcaactggca gaacagcaaa aacagctggc
     7561 cgaccctgct ttccgcgctc gtgcgcaagg tttagcggcg gtggactctg gcatggcggg
     7621 taaagccatt cccgaactac aacaggcggt gcgggcgaac ccgaaagaca gtgaggctct
     7681 gggggcgctg ggccaggcgt attcccagaa aggtgatcgc gccaatgcag tggcgaatct
     7741 ggaaaaagcc ctcgcgctgg atccgcacaa cagcaataac gacaaatgga acagtctgct
     7801 gaaagtaaac cgctactggc tggcgatcca gcagggcgat gccgcgctga aagccaataa
     7861 tcctgaccgg gcagaacggc tgttccagca ggcacgtaat gtcgataaca ccgacagcta
     7921 tgcagtgctt gggctgggcg atgtggcgat ggcgcgcaaa gattatcccg ccgccgaacg
     7981 ctattaccag cagaccttgc gtatggacag cggcaacact aacgccgtgc gcgggctggc
     8041 gaatatttac cgccagcagt cgccagaaaa agccgaagcg tatatcgcct cgctctctgc
     8101 cagccagcgg cgcagcattg atgatatcga acgcagcctg caaaacgacc gtctggcaca
     8161 gcaggcagag gcactggaaa accaggggaa atgggcgcag gcggcagcac ttcagcggca
     8221 acgactggcg ctggaccccg gcagtgtatg gattacttac cgactttcgc aggatctctg
     8281 gcaagccgga caacgcagcc aggccgatac gttaatgcgc aatctggcgc agcagaagcc
     8341 gaacgacccg gagcaggttt acgcttacgg gctgtacctc tccggtcatg atcaggacag
     8401 agcggcgctg gcgcatatca acagcctgcc gcacgcgcag tggaacagca atattcagga
     8461 gctggttaat cgactgcaaa acgatcaggt gctggaaacc gctaatcgcc tgcgagaaaa
     8521 cggtaaagag gcagaagcgg aagcgatgct gcgccagcaa ccgccttcct cgcgtattga
     8581 tctgacgctg gctgactggg cgcagcagcg acgtgattac accgccgccc gcgctgcata
     8641 tcagaatgtt ctgacgcggg agccaactaa cgccgatgcc attcttggtc tgacggaagt
     8701 ggatattgct gccggtgaca aagcggcggc acgtagccag ctggcgaaac tgcctgccac
     8761 tgataacgcc tcgctgaaca cacagcggcg cgtggcgctg gcacaggcgc agcttggcga
     8821 taccgcagcg gcgcagcaga catttaataa gttgatcccg caggcaaaat ctcagccacc
     8881 gtcgatggaa agcgcgatgg tactgcgtga tggtgcgaag tttgaagcgc aggcgggcga
     8941 tccaacgcag gcgctggaaa cctacaaaga cgccatggtt gcatccggtg tgaccacgac
     9001 gcgtccgcag gataacgaca cctttacccg gctgacccgt aacgacgaga aagatgactg
     9061 gctgaaacgc ggcgtgcgca gcgatgcggc ggacctctat cgccagcagg atcttaacgt
     9121 cactctcgag cacgattact ggggttcgag cggcaccggt ggttactccg atctgaaagc
     9181 gcacaccact atgttgcagg tggacgcgcc ctattctgac gggcggatgt tctttcgcag
     9241 tgatttcgtc aatatgaacg tcggcagttt ctccactaat gccgatggca aatgggatga
     9301 caactggggc acctgtacat tacaggactg tagcggcaac cgcagccagt cggattccgg
     9361 tgccagcgtg gcggtcggct ggcgaaatga cgtctggagc tgggatatcg gtaccacgcc
     9421 gatgggcttc aacgtggtgg atgtggtcgg cggcatcagt tacagcgatg atatcgggcc
     9481 gctgggttac accgttaacg cccaccgtcg gcccatctcc agttctttgc tggcctttgg
     9541 tgggcaaaaa gactctccga gcaataccgg gaaaaaatgg ggcggcgtgc gtgccgacgg
     9601 cgtggggcta agtctgagtt acgataaagg tgaagcaaac ggcgtctggg catcgcttag
     9661 cggtgatcag ttaaccggta aaaatgtcga agataactgg cgtgtgcgct ggatgacggg
     9721 ctattactat aaggtcatca accagaacaa tcgccgcgtc acaatcggcc tgaacaacat
     9781 gatctggcat tatgacaaag acctgagtgg ctactcactc ggacagggcg gttactacag
     9841 tccgcaggaa tacctgtcgt ttgccatacc ggtgatgtgg cgggagcgca cggaaaactg
     9901 gtcgtgggag ctgggggcgt ctggctcgtg gtcgcattca cgcaccaaaa ccatgccgcg
     9961 ttatccgctg atgaatctga tcccgaccga ctggcaggaa gaagctgcgc ggcaatccaa
    10021 cgatggcggc agcagtcagg gctttggcta cacggcgcgg gcattacttg aacgacgtgt
    10081 tacttccaac tggtttgtcg gcacggcaat tgatatccag caggcaaaag attacgcacc
    10141 cagccatttc ctgctctacg tacgttattc cgccgccgga tggcagggtg acatggattt
    10201 accgccgcag ccgctgatac cttacgccga ctggtaagtt ttcagatagc gcctctctta
    10261 atgccgctgc gatcgggtat actcgggcgg caatctggga tttccggggg gagacaattt
    10321 gcgcgtaagt cgctcgttaa caatcaagca gatggcaatg gtggcagccg ttgtcctggt
    10381 gttcgttttt attttttgca ccgttttgct gttccatctg gtccagcaga atcgctataa
    10441 cacggctacg caactggaaa gcattgctcg ctctgtccgc gaacccttat cttctgccat
    10501 tttgaaaggc gatattcccg aagcggaagc tattcttgcc agcattaaac cggccggcgt
    10561 ggtcagccgt gccgatgtag tgctgcccaa ccagttccag gcgctgcgta aaagttttat
    10621 tccagagcgt ccggtgccgg taatggttac tcgcctgttt gagctaccgg tgcaaatctc
    10681 gctgggcgtt tactcgctgg aacgtccggc aaacccgcag ccaattgcct atctggtgtt
    10741 acaggcggat tccttccgca tgtataagtt cgtgatgagc accctctcaa cgttagtgac
    10801 catttactta cttttgtcgt taatattgac ggtggcgatt agctggtgca ttaaccgcct
    10861 gattttgcat ccgttacgca atattgctcg cgaacttaac gccatcccac cccaggagct
    10921 tgttggtcac caactggcat taccgcgtct gcatcaggac gatgaaatcg gtatgttggt
    10981 gcgcagttac aacctcaacc agcaattgct gcagcgccat tatgaagaac agaacgaaaa
    11041 tgcgatgcgc ttcccggtgt cggatttgcc gaacaaagcc ttgctgatgg agatgctgga
    11101 gcaggtggtg gcgcgtaaac aaaccaccgc gctgatgatc atcacctgtg aaaccctgcg
    11161 tgatactgcg ggtgtgctga aagaggcgca acgagaaatt ctgctgctga cgctggtgga
    11221 aaaactcaaa tcggtactgt cgccacgcat gatcctcgcg cagattagcg gttatgactt
    11281 tgctgtcatt gccaacggtg tacaggaacc gtggcacgct atcacattag gtcagcaagt
    11341 gctcactatc atgagcgagc gcctgccgat tgaacgtatt caactccgtc cgcactgtag
    11401 cattggcgtg gcgatgttct acggcgatct caccgccgaa cagctttaca gtcgcgctat
    11461 ttctgcggca tttaccgctc gccataaagg caaaaatcag attcagttct ttgatccgca
    11521 gcagatggaa gccgcccagc agcggttgac ggaagagagc gatatcctta atgcactgga
    11581 aaatcatcaa tttgcaattt ggttacagcc acaggtcgag atgaccagcg gtaaactggt
    11641 cagtgcggaa gtgttactgc gtatccagca accggatggc agttgggacc tgcccgatgg
    11701 cttaatcgat cgcattgagt gctgtgggct gatggttacc gtcggtcact gggtgctgga
    11761 agagtcctgt cgattgcttg ctgcctggca agagagcggc attatgctgc ccttgtcggt
    11821 aaacctctca gcgctgcaac tgatgcatcc aaatatggtg gcggatatgc tggaactgtt
    11881 aacccgctat cgcattcagc cgggaacact gattctggaa gtgacagaaa gccgacgtat
    11941 tgacgatcct catgctgcgg tggcaatcct ccgtccgctg cgcaatgccg gagttcgggt
    12001 ggcgctggat gatttcggca tgggctacgc agggctgcgt cagctgcagc atatgaaatc
    12061 gttgccaatc gacgtactga aaatcgacaa aatgtttgtt gaaggtttgc cggaagatag
    12121 cagcatgatt gctgcaatta tcatgctggc gcagagcctg aacttacaaa tgattgccga
    12181 aggcgtggag actgaagcac aacgcgactg gctggcaaaa gcgggcgttg gtattgccca
    12241 gggcttcctt tttgctcgcc cactccctat tgaaatcttc gaagagagtt acctggaaga
    12301 aaagtagcta ccccaaactg attacaaaac tttaaaaagt gctggtttgt gcgagccagc
    12361 tcaaactttt taaccttttt gtttcaatta tgatccaggt acatttctgt gatgttgtct
    12421 gggtgttatt ttaaggccgc aggtacccca taaccttaca agacctgtgg ttttactaaa
    12481 ggacacccta tgaaaacctc tctgtttaaa agcctttact ttcaggtcct gacagcgata
    12541 gccattggta ttctccttgg ccatttctat cctgaaatag gcgagcaaat gaaaccgctt
    12601 ggcgacggct tcgttaagct cattaagatg atcatcgctc ctgtcatctt ttgtaccgtc
    12661 gtaacgggca ttgcgggcat ggaaagcatg aaggcggtcg gtcgtaccgg cgcagtcgca
    12721 ctgctttact ttgaaattgt cagtaccatc gcgctgatta ttggtcttat catcgttaac
    12781 gtcgtgcagc ctggtgccgg aatgaacgtc gatccggcaa cgcttgatgc gaaagcggta
    12841 gcggtttacg ccgatcaggc gaaagaccag ggcattgtcg ccttcattat ggatgtcatc
    12901 ccggcgagcg tcattggcgc atttgccagc ggtaacattc tgcaggtact gctgtttgcc
    12961 gtactgtttg gttttgcgct ccaccgtctg ggcagcaaag gccaactgat ttttaacgtg
    13021 atcgaaagtt tctcgcaggt cattttcggc atcatcaata tgatcatgcg tctggcacct
    13081 attggtgcgt tcggggcaat ggcgtttacc atcggtaaat acggcgtcgg cacactggtg
    13141 caactggggc agctgattat ctgcttctac atcacctgta tcctgtttgt ggtgctggta
    13201 ctgggttcaa tcgctaaagc gactggtttc agtatcttca aatttatccg ttacatccgt
    13261 gaagaactgc tgattgtact ggggacttca tcttccgagt cggcgctgcc gcgtatgctc
    13321 gacaagatgg agaaactcgg ctgccgcaaa tcggtggtgg ggctggtcat cccgacaggc
    13381 tactcgttta atcttgatgg cacatcgata tacctgacaa tggcggcggt gtttatcgcc
    13441 caggccacta acagtcagat ggatatcgtc caccaaatca cgctgttaat cgtgttgctg
    13501 ctttcttcta aaggggcggc aggggtaacg ggtagtggtt ttatcgtgct ggcggcgacg
    13561 ctctctgcgg tgggccattt gccggtagcg ggtctggcgc tgatcctcgg tatcgaccgc
    13621 tttatgtcag aagctcgtgc gctgactaac ctggtcggta acggcgtagc gaccattgtc
    13681 gttgctaagt gggtgaaaga actggaccac aaaaaactgg acgatgtgct gaataatcgt
    13741 gcgccggatg gcaaaacgca cgaattatcc tcttaatctc accacttatg cccgcactcc
    13801 cttccccgag tgcgggtgtt tgcgcataat tgacccctgt gcctgaactt accacctggg
    13861 tgagttcagg tttatttcac ttcttccgtg acattttcat gttcttgcgg tctaacacga
    13921 agtgttttta acgtcattta gactgaccga caacgggcag ttttttatta ccaggatagt
    13981 tgatcagggg ttcacatgca gggcacaaaa attcgacttt tagcgggcgg tttgctgatg
    14041 atggccactg ctggctatgt gcaggcagat gcgctccagc ctgatccagc atggcaacag
    14101 gggacgcttt ccaacggttt acagtggcaa gtgctgacta caccccagcg tcccagcgat
    14161 cgtgttgaaa ttcgcctgct ggttaatacc ggttcgctcg ccgaaagtac acaacagagc
    14221 ggttacagtc acgccatccc tcgtattgcg ctaacgcaaa gcggtggcct tgacgcagcg
    14281 caggcgcgtt cattgtggca gcaggggatc gaccctaaac gcccgatgcc gccggtaatt
    14341 gtctcttatg acaccacgct gtttaatctg agtttgccca ataaccgtaa cgacttgctg
    14401 aaagaggcgc tctcttatct ggcaaatgcc actggcaaac tgactatcac gccagaaacc
    14461 atcaaccatg cgttgcaaag tcaggacatg gtggcaacct ggcctgccga tactaaagag
    14521 ggctggtggc gttatcgtct gaaaggatca accttgttag gtcacgatcc tgccgatccg
    14581 ctgaaacaac ccgttgaagc ggaaaagatt aaagatttct atcagaaatg gtacaccccg
    14641 gatgcaatga cgctgctggt ggtgggaaac gtggatgcgc gctcggtcgt cgaccaaatc
    14701 aataaaacgt ttggcgaact gaaaggcaaa cgtgaaacac cggctccggt gccgacgctt
    14761 tctccgctgc gtgcggaagc ggtgagtatt atgaccgacg cggtgcgtca ggaccggcta
    14821 tctatcatgt gggatacgcc gtggcagccg attcgtgaat cagccgcact gctgcgctac
    14881 tggcgtgcgg acctggcccg tgaggcgctg ttctggcatg ttcagcaagc gttaagcgcc
    14941 agtaacagta aagacatcgg tcttggattt gactgtcgtg tgctgtatct gcgtgcgcag
    15001 tgtgccatca acatcgaatc accaaacgac aagctgaaca gcaaccttaa tctggtggcg
    15061 cgtgaactgg cgaaggttcg cgataaaggt ctgccggaag aagagttcaa tgcgttagtg
    15121 gcgcaaaaga aactggagct gcagaaactg tttgccgcct atgcgcgagc tgataccgat
    15181 attctgatgg ggcagcggat gcgttcgttg caaaatcagg tcgtcgatat cgcgccggag
    15241 caatatcaga aactgcgtca ggatttcctt aacagcctga cggtggagat gttaaatcag
    15301 gatctgcgtc agcagttgtc gaatgatatg gcgttaatac tgctgcagcc gaaaggcgag
    15361 ccggaattta acatgaaagc gttgcaggcg gcctgggatc aaatcatggc cccatcgact
    15421 gcggctgccg ccacatctgt cgccacggat gacgtacatc ctgaagtgac ggatattcca
    15481 cctgtacagt aatgcctgat ggtgatgagt tagcacaacc atcaggtatt tatggatgag
    15541 ctggatagag gggtaacact ctatccagcc ttttgcatat gctgctttta cgctggcatc
    15601 gcttcacgcg ggataatcgc gccgcgatac tgaataacgg tacttgcggt caggtgcccg
    15661 cgtttcgctg cgtcttccgc actgccgccc gtcaaacgta ccgccagata accggcactg
    15721 aaagagtcgc cagctgcggt ggtatcgatc actttttctt tcggcagttt taccgccgga
    15781 acatccacta acccttcacc agcaatggac accaggcaag aatccgcccc gcgtttcacc
    15841 accacttctt tcacgcccgc gttatgggtg cgcgcaatga cgtcttccac cggctgttga
    15901 ccccacagcg cgtcttcatc gtccagcgtc aggaaggcga tatccgtgca ttccagcatt
    15961 tgttggtaca cctgctgtgt ctcttctttg ctggcccaca ggcgcggacg atagttattg
    16021 tcgaaaatca cttttccgcc gttggcgcgg cattcgcgca gcagggaaag cagcttttcg
    16081 cggctggtcg ggcttaagat cgccaggcta atcccgctca ggtagagata atcgaaattc
    16141 gccagctctt cgcaaatcgc cgcggattgc tcactctcca gccagaattt ggcggcggct
    16201 tcgttacgcc agtagtagaa cgtacgttcg ccggtgctgt cggtttcaat gtagtaaagg
    16261 cccggcagac ggttttccat ccgttgggtc agggaagtat caacgttctc gccgcgccag
    16321 gcgtccagca tctgctggct aaaactgtcc gttcccagcg ccgttacgta atgaacggtt
    16381 aatgccgcag gatcgacctg acgggcgata tagacggaag tgttcagggt atcgccgccg
    16441 aaaccgcgct taacgtccgc gcctttctcg gaaagctcaa tcatgcattc gccaatcacg
    16501 gcaatctttt tggacatagt cgtgaacctg atctgtaaaa aattagcgtt agtttgcgct
    16561 gtggtggttt gctggtcaac catattaaaa cagtgttcca ttattttttt gagccagaac
    16621 gtattcctga aagattccgt tgtggagtgg ggaaatttac cccggccagg cggtaaagtt
    16681 ctgcccttgc gcgccgataa tctttgtcga gtccgggcag catcactttt aaacacagga
    16741 catctttgat gataaggcag gttatccagc gaataagcaa ccctgaagca agcatcgaga
    16801 gcttgcagga acggcgtttt tggttgcagt gtgagcgtgc ttacacctgg cagccgatct
    16861 atcaaacatg cgggcggtta atggccgtgg agctattaac ggtggtcacg catcccttga
    16921 acccttcgca acgcctgccg ccggatcgct attttactga aatcaccgtt agccatcgga
    16981 tggaggttgt gaaagagcag attgatttgc tggcgcaaaa agccgacttc tttgtagagc
    17041 acggcctgct ggcatcggtc aatattgatg gccctacgct catcgccctg cgtcagcaac
    17101 caaaaatcct gcgccagatt gaggatcttc cctggctgcg tttcgaactg gtggaacaca
    17161 tccgcctgcc gaaagattca acctttgcct cgatgtgtga atttggcccg ctgtggctgg
    17221 atgattttgg taccgggatg gcaaatttct ctgcactaag tgaagtgcgt tatgactaca
    17281 tcaaaatcgc gcgagaactg tttgtgatgc tgcgtcagtc gccggaagga cgcacactct
    17341 tttctcagct tttacatcta atgaatcgct attgtcgcgg ggtgattgtc gagggcgtag
    17401 aaacaccgga agagtggcgt gatgttcaga actcgcccgc attcgccgca caaggctggt
    17461 ttctttcacg cccggcaccg atagaaacgc tgaatacggc ggttctggcg ctataagctg
    17521 cctcattttc cgcctggctg gactatcttt aggactggca caggaagaat atgaggaagc
    17581 gaacgatgag caaggcaggc aaaataaccg ctgcgatttc aggggctttc ttgttgttga
    17641 ttgtcgtggc gatcattttg attgcaactt ttgactggaa tcgactcaaa ccgaccatca
    17701 accagaaagt ctctgcggag ttgaatcgtc cgttcgctat ccgtggcgat ctgggcgtgg
    17761 tgtgggagcg gcaaaaacaa gaaactggct ggcgcagctg ggtgccgtgg ccccatgtac
    17821 acgcggaaga catcattctt ggcaatccac cggatattcc cgaagtcaca atggtgcatt
    17881 tgccgcgagt agaagcgacg ctggccccgc tggcgctgct gaccaaaacg gtctggttgc
    17941 cgtggatcaa gctcgaaaag cccgacgcgc gcctgattcg cctctccgaa aagaacaata
    18001 actggacgtt taatctcgcc aacgatgaca acaaagacgc gaatgcaaag ccgtcggcgt
    18061 ggtcgtttca gctggataat attcttttcg atcaagggcg gatcgccatt gatgacaaag
    18121 taagcaaagc ggatctggaa atttttgtcg atccgttagg caagccgctg ccgttcagcg
    18181 aagttaccgg atcgaaaggt aaagcggata aagaaaaggt gggcgattac gtttttggcc
    18241 tgaaggcgca gggacgttat aacggtgaac cgctcactgg tacgggaaaa ataggcggta
    18301 tgctggcgct gcgtggcgaa ggaacgccgt ttccggtaca ggctgatttc cgctctggta
    18361 acacccgtgt tgcttttgat ggcgttgtga atgacccaat gaagatgggg ggtgtcgatt
    18421 tacggcttaa attttctggc gattcgctgg gtgatctcta tgaactgacg ggcgttctgc
    18481 tgcccgatac accgccgttt gaaacggatg gtcggctggt agcgaaaatc gacactgaaa
    18541 aatcgtcggt ctttgattat cgcggtttta atgggcgcat tggcgatagc gatatccacg
    18601 gttctctgat ctataccacc ggcaagccac gaccaaaact ggaaggtgat gtcgagtcgc
    18661 ggcaattgcg gctggcggac ctgggaccgt tgattggcgt tgattccggg aaaggggcag
    18721 aaaagtcgaa acggtctgaa cagaagaagg gtgaaaaaag cgttcagcct gcgggcaaag
    18781 tgctgcctta tgaccgcttc gaaaccgata aatgggacgt catggatgcc gatgttcgct
    18841 ttaaagggcg gcgcattgag catggcagta gcctgccgat tagcgatctt tctactcata
    18901 tcatcctcaa aaatgctgac ctgcgcctgc aaccgctgaa atttggcatg gcgggcggca
    18961 gcattgcggc gaatattcat ctggaaggcg ataaaaagcc gatgcagggg cgggcagata
    19021 ttcaggctcg tcggctgaaa ctgaaagaac tgatgcccga tgtagaactg atgcagaaga
    19081 cgctggggga aatgaacggt gacgcggaac tgcgcggtag cggtaactcg gtggcggcgc
    19141 ttttaggcaa cagtaacggc aacctgaaac tgttgatgaa tgacgggctg gtgagccgca
    19201 acctgatgga gattgttggg ctgaatgttg gcaactacat tgtcggtgcg atatttggtg
    19261 atgatgaggt gcgggtgaac tgcgcggcgg cgaatctgga tattgccaac ggcgtggcgc
    19321 gcccgcagat ttttgctttc gatactgaga acgcgttgat taacgttacc ggtacggcaa
    19381 gttttgcttc ggaacagctg gatttaacta tcgatccaga aagtaaaggg attcgcatta
    19441 tcacgctgcg ttcgccgctg tatgtgcgtg ggacgtttaa aaatccgcag gctggggtga
    19501 aagccgggcc gttgattgcc cgtggtgctg ttgcggcggc actggcaacg ctggttacgc
    19561 cagcggcagc attgctggca ctgatctcac cttccgaagg ggaggctaat cagtgccgga
    19621 caattttgtc gcagatgaag aagtgatgat agttgccgga tgtctgacat ccggcagtta
    19681 aaacgactac aacgactggt gccgcgtctc atgcgttaac agcagggcaa ttaacgtcag
    19741 tccggccatc gccgccagat ataaccccac cgcgcctaac ccatagttag tctgcaacca
    19801 ggccgcgata tacggcgcaa cagacgcccc gagaatcgac gatacgttat aggagaacga
    19861 tgctccggta taacggactt ccgtcgggaa cagctccggc agcagcgccc ccatcggacc
    19921 aaaggtcagg cccatcaaac tcaagcccag cagcaggaaa acaaacacca atgctggatt
    19981 accagaaccc aacagtgggt taaaggcaaa cagggcaaac agaatgataa gcgtagtgat
    20041 caccaccata cttttacggc gaccaaaagc atctgccagc agacccgcca acggaaccat
    20101 cactgcaaaa ccgataaccg ccatcatcaa catccacagt acttcgttac gcggtaaacc
    20161 aaggccaaca ggcgccgctg cggtactaaa ggtcatagag tagaccgtca tgatgtaaaa
    20221 cagcgtatag gttgccagca taatgaacgt acccagtacg gttacgcgaa catgtttggt
    20281 cagcagcgta cccagcggga tcttcacctg ttttttcgct ttagcgactt tctcaaacac
    20341 cggcgactca tgcagcgaca cgcgaacata caggccgata atgaccagca ccgccgagaa
    20401 gataaatggc acacgccagc cccagctcat aaactgctcg tcggtcagta gccaggaaag
    20461 cagcaagaaa gtgccgttgg caaagaagaa gccaatcggt gcgcccagct gcgggaagga
    20521 gccgtacaat gcacgtttgc gcggtggggc gttttcagtc gccagcagcg ccgcgccgcc
    20581 ccattcacca cctaagccca gaccctgacc aaatcgagcc agcgccagca gcagcggggc
    20641 gaaaatacca atcgtggcat agcctggcag cagaccaatc accacggtcg aaatccccat
    20701 cgttagcaac gaggcgacca gcgtcgcttt acgcccaacg cgatcgccaa aatgaccaaa
    20761 aacggcagag ccaatggggc gcgcgacgaa ggcgatggcg aaggtggcga gcgactgtag
    20821 cgttgctgcc gttggatcgc cctgcgggaa gaagatatgc ggaaacacaa taacggccgc
    20881 agtggcgtaa atgtaaaagt cgaagaactc aatggccgtg ccaatgagag aggcgacaag
    20941 gactttatta cgcgagttga tcggcgtgta ttcttgctcg tggtcgagtg ttgtggctgt
    21001 tgcttgcata atcttttctt attttttggc taacgaatag ccatattacg cacagcaaaa
    21061 gtgatatttc aatgtgtaac aaatcacgga aatggtcaaa aatcaggcaa aaaggggata
    21121 atgtttaagc cagtgatttt tcttctatga aatgcttcac ataattttat aaatggtgga
    21181 ttaaactggt tttaggttaa ataaaagtta ccaactggat ttatatgctg gggttatgag
    21241 ttggaatgaa attgtttgct cctgaataga tggctaaaga ggctctggga aagagtttca
    21301 agaggttatg tgtgcattat agccggatgc gatgctggcg catcttatcc ggcctacggg
    21361 gagccaggcc gaataaattc gtacgccgca tccggcctat tttaaggctg cgttttcccc
    21421 ggcattcgcg ggtcgtcttt atattcggcg gtggcaatcc acgccgcgca aaacagcgtc
    21481 aaacgggcga agaagtagaa aaatgccatc agccccagca cggaaccaaa tgctgcgcca
    21541 gagggtgatt tcatcaacga tggcagggtg taggtcatca cgattttaat cacttcaaaa
    21601 ccaatagcag ccagaaatgt tccgcggatc agggcttttt tgcgcggacg gtggcgcggc
    21661 aaccgccaga agatccagaa gaaaagcaga tagttggcga agatggaaat cgccaggcca
    21721 atcaaccgcc atgtcggttt cagccactca atgctgttca ggtgcagggc gctaataatc
    21781 atttgctgcg cagaaccggc aaccgaggtg atagaaagcg tcacaatcag cgcaatcaac
    21841 aaaccaatca gcgaaataaa atcacgcaga tattttaccc agaacttctc ctgatcttgc
    21901 ggcgagcgtt cccagacatc gcgtgactgg gcgcgaatcg cttcacgcag gttacccatc
    21961 cagttgatgc cggaataaag cgccactgcc agaccgacaa gccctacagt cgtacgctgc
    22021 tgaacggcgg tgttgatggt gtttttcaac gtggcggcta gcgtcggatc gctaatgttt
    22081 tgcagaattt tgtcgaagat atcctgtagc aacatcggat gggaggccag cacaaagccc
    22141 cctgcggcaa acgacaccat caaaatgggg atcatcgaca aaaatgagaa ataggtgata
    22201 gccgccccaa actggttacc cagccgatcg ttaaagcgtt ctgtcgcgcg aatcaggtgc
    22261 gcaataaccg gttggcgttg gactttctcg gcagtggtgg tgacggtttc cagcgcctgg
    22321 ctaactttgc cgcctttctc gctattatcc aactgcttaa tcggctcgtg ctccagatcc
    22381 tgggtgggac gtttgatctc gttttcctgc gtcatccgct cttttgtcct tctgtcgtgg
    22441 ttattcttaa aaagtatagt cggtcagtcc aggtgctctt tcattacgcc agccagccac
    22501 tgcataaaca ggtttacacg ccgggaaagc tcccgacgct gcggataaac cagtgaaagg
    22561 gacagcggct cggcacggta gccaggcaac acttcaataa gccgcccggc acgcagggct
    22621 tcgcgcacgg caatgcgcgg gatctgaata atccccagac cggcaatacc ggcggcgaga
    22681 tagttttcgc tactgtttac cgtcaacatg ccgccagact taaaccactg gacgccatta
    22741 acgctggcaa cctcaaaacc taacggatgt acacccaggt gcggtgtata acgcactatt
    22801 gcgtgtgaag tcagatcatc ggggctttgc ggataaccaa agcgcgtcag atagtgtgga
    22861 ctggcacagt tgaccatggt cagtttgccg agagaacggg cgataacgcc atcttccggt
    22921 aatgcgccag tgcgtatcac gcaatcaaaa ccatcatgaa gaatatctac cggacggtca
    22981 tggctactca gttccagctc aattcccgga tgcagataga gaaattccga caagcgcggc
    23041 agtaacaggc ttttcgcgat tcctgacggg atgtcgatgc gtaatttacc gctgatactg
    23101 gtggcatcct gttgaaacag accgtccagt tcgctgagat tactcaacac atcttttgcc
    23161 cgttgataat aggtcatgcc ttctggcgtg agtttgaccc gtcgcgtggt gcgctggaga
    23221 agctgggtgc caagttgatg ttccagtgcc tgtatctggc gcgaaacact tccctttggc
    23281 aaagcaaaga aatccgccgc gcgggaaaaa ctttccagct ccgcgacttt gatgaacaac
    23341 tgcattgcgt gaattttatc catttctttg accgattgtt gttcacaacg aaacagtagc
    23401 gcgcatatcg cattgtttat tgttttttct aacatctaat gccgggtttc acaatgttgt
    23461 catgatgaat gtggcgattt ctcagcagga aaagggcgga atggctttat gagaaaggtg
    23521 aattccctta aacagagcgt atttttcagc gattcctttt aaagtgaata gaagatgtat
    23581 tggctatttg tgcttatttt tatctcattg ccgtttgtta tttttgttta tggtcgcgga
    23641 atttcatttt ta
//
LOCUS       DAZFJL010000076        22991 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFJL010000076 DAZFJL010000000
VERSION     DAZFJL010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.076"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            73..1026
                     /locus_tag="ACHJ8C_002817"
     CDS             73..1026
                     /locus_tag="ACHJ8C_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5552650.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1067..2062)
                     /gene="pgl"
                     /locus_tag="ACHJ8C_002818"
     CDS             complement(1067..2062)
                     /gene="pgl"
                     /locus_tag="ACHJ8C_002818"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO5552651.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHLGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWGVVNAH"
     gene            2217..3035
                     /gene="ybhA"
                     /locus_tag="ACHJ8C_002819"
     CDS             2217..3035
                     /gene="ybhA"
                     /locus_tag="ACHJ8C_002819"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO5552652.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYRLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHNI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQNFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(3036..4094)
                     /gene="modC"
                     /locus_tag="ACHJ8C_002820"
     CDS             complement(3036..4094)
                     /gene="modC"
                     /locus_tag="ACHJ8C_002820"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO5552653.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDADKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVNQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(4097..4786)
                     /gene="modB"
                     /locus_tag="ACHJ8C_002821"
     CDS             complement(4097..4786)
                     /gene="modB"
                     /locus_tag="ACHJ8C_002821"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO5552654.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGLSFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDIKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4786..5559)
                     /gene="modA"
                     /locus_tag="ACHJ8C_002822"
     CDS             complement(4786..5559)
                     /gene="modA"
                     /locus_tag="ACHJ8C_002822"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5552655.1"
                     /translation="MARKWLNLFTGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5726..5875)
                     /gene="acrZ"
                     /locus_tag="ACHJ8C_002823"
     CDS             complement(5726..5875)
                     /gene="acrZ"
                     /locus_tag="ACHJ8C_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO5552656.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            6004..6792
                     /gene="modE"
                     /locus_tag="ACHJ8C_002824"
     CDS             6004..6792
                     /gene="modE"
                     /locus_tag="ACHJ8C_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO5552657.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AVTAQSGARLGLDEGKEVLILLKAPWVGITQDDAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6860..8332
                     /gene="modF"
                     /locus_tag="ACHJ8C_002825"
     CDS             6860..8332
                     /gene="modF"
                     /locus_tag="ACHJ8C_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO5552658.1"
                     /translation="MSSLQILQGTFRLGDTKTLQLPRLTLNAGDSWAFVGSNGSGKSA
                     LARALSGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDTPRCMQLAQQFGITALLNRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSELLEGVQMPEPDEPSVRHALPAHEPRIVLNNGVVSYNERPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGDFYRYALTKIN"
     gene            8594..9610
                     /gene="galE"
                     /locus_tag="ACHJ8C_002826"
     CDS             8594..9610
                     /gene="galE"
                     /locus_tag="ACHJ8C_002826"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO5552659.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9620..10666
                     /gene="galT"
                     /locus_tag="ACHJ8C_002827"
     CDS             9620..10666
                     /gene="galT"
                     /locus_tag="ACHJ8C_002827"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO5552660.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGETYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEDNQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10670..11818
                     /gene="galK"
                     /locus_tag="ACHJ8C_002828"
     CDS             10670..11818
                     /gene="galK"
                     /locus_tag="ACHJ8C_002828"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO5552661.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALFPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11812..12852
                     /gene="galM"
                     /locus_tag="ACHJ8C_002829"
     CDS             11812..12852
                     /gene="galM"
                     /locus_tag="ACHJ8C_002829"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO5552662.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILAEEYLPVDEGGIPHDGLKSVAGT
                     SFDFRNAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            13055..13807
                     /gene="gpmA"
                     /locus_tag="ACHJ8C_002830"
     CDS             13055..13807
                     /gene="gpmA"
                     /locus_tag="ACHJ8C_002830"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO5552663.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(13973..15025)
                     /gene="aroG"
                     /locus_tag="ACHJ8C_002831"
     CDS             complement(13973..15025)
                     /gene="aroG"
                     /locus_tag="ACHJ8C_002831"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO5552664.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCTDVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLANAVKARRG"
     gene            15341..15721
                     /locus_tag="ACHJ8C_002832"
     CDS             15341..15721
                     /locus_tag="ACHJ8C_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO5552665.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQSNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15835..16776
                     /gene="zitB"
                     /locus_tag="ACHJ8C_002833"
     CDS             15835..16776
                     /gene="zitB"
                     /locus_tag="ACHJ8C_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO5552666.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16773..17492)
                     /gene="pnuC"
                     /locus_tag="ACHJ8C_002834"
     CDS             complement(16773..17492)
                     /gene="pnuC"
                     /locus_tag="ACHJ8C_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO5552667.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17530..18573)
                     /gene="nadA"
                     /locus_tag="ACHJ8C_002835"
     CDS             complement(17530..18573)
                     /gene="nadA"
                     /locus_tag="ACHJ8C_002835"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO5552668.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVEMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVS
                     LNRMLDFAATLRG"
     gene            complement(18851..18926)
                     /locus_tag="ACHJ8C_002836"
     tRNA            complement(18851..18926)
                     /locus_tag="ACHJ8C_002836"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18891..18893),aa:Lys,seq:ttt)
     gene            complement(18960..19035)
                     /locus_tag="ACHJ8C_002837"
     tRNA            complement(18960..19035)
                     /locus_tag="ACHJ8C_002837"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19000..19002),aa:Lys,seq:ttt)
     gene            complement(19084..19159)
                     /locus_tag="ACHJ8C_002838"
     tRNA            complement(19084..19159)
                     /locus_tag="ACHJ8C_002838"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19124..19126),aa:Lys,seq:ttt)
     gene            complement(19163..19238)
                     /locus_tag="ACHJ8C_002839"
     tRNA            complement(19163..19238)
                     /locus_tag="ACHJ8C_002839"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19203..19205),aa:Val,seq:tac)
     gene            complement(19290..19365)
                     /locus_tag="ACHJ8C_002840"
     tRNA            complement(19290..19365)
                     /locus_tag="ACHJ8C_002840"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19330..19332),aa:Lys,seq:ttt)
     gene            complement(19368..19443)
                     /locus_tag="ACHJ8C_002841"
     tRNA            complement(19368..19443)
                     /locus_tag="ACHJ8C_002841"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19408..19410),aa:Val,seq:tac)
     gene            complement(19479..19554)
                     /locus_tag="ACHJ8C_002842"
     tRNA            complement(19479..19554)
                     /locus_tag="ACHJ8C_002842"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19519..19521),aa:Lys,seq:ttt)
     gene            complement(19718..20509)
                     /gene="cpoB"
                     /locus_tag="ACHJ8C_002843"
     CDS             complement(19718..20509)
                     /gene="cpoB"
                     /locus_tag="ACHJ8C_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO5552669.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGVAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(20519..21040)
                     /gene="pal"
                     /locus_tag="ACHJ8C_002844"
     CDS             complement(20519..21040)
                     /gene="pal"
                     /locus_tag="ACHJ8C_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO5552670.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYAKNRRAVLVY"
     gene            complement(21075..22367)
                     /gene="tolB"
                     /locus_tag="ACHJ8C_002845"
     CDS             complement(21075..22367)
                     /gene="tolB"
                     /locus_tag="ACHJ8C_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO5552671.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(22500..>22991)
                     /gene="tolA"
                     /locus_tag="ACHJ8C_002846"
     CDS             complement(22500..>22991)
                     /gene="tolA"
                     /locus_tag="ACHJ8C_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO5552672.1"
                     /translation="AADKKAAAEKAAADKKAAAAKAAAEKAAAAKAAAEADDIFGELS
                     SGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAG
                     KTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLD
                     FKP"
BASE COUNT         5714 a         6043 c         5705 g         5529 t
ORIGIN      
        1 gttggctaaa tgcagttaaa atttttaact gccagtcacc caaaatcgtc gcttccattt
       61 tccggagtaa taatgaagca tgaattatca agtatgaagg catttgtcat actggcagag
      121 tccagttcat ttaacaatgc tgctaaatta ctcaatatta cgcaacctgc attaacgcga
      181 agaataaaaa agatggagga agatttacac attcaactat ttgagcgtac aactcgcaag
      241 gttacgttaa caaaagcagg aaaaaggttg ctcccggagg cgcgggaatt aataaaaaaa
      301 ttcgatgaaa cgctttttaa tattcgtgat atgaatgctt atcatcgtgg tatggtgacg
      361 ttagcgtgta ttccgaccgc agtattttat tttttaccgc tggcaatcgg taaatttaat
      421 gagctgtatc ccaatattaa agtgcggatt ctggaacaag gcacgaataa ttgcatggaa
      481 tcggtgctgt gtaacgaatc agactttggc ataaacatga ataacgtcac aaattcatcc
      541 attgatttta ccccactggt caacgagccg ttcgtgctgg cctgtcggcg tgaccatcca
      601 ttggccaaaa agcagcttgt agaatggcag gagttagtgg gttacaaaat gattggcgta
      661 cgctcctcca gcggcaaccg actattgata gaacaacagc tagccgacaa gccctggaag
      721 ctggactggt tttacgaagt gcgtcatctt tcgacgtcat tagggctggt ggaggcggga
      781 ctggggattt cggcgcttcc tggtctggca atgccccatg cgccctattc ttcgattatt
      841 ggtatcccac tggtggaacc cgttatacgt cggacattgg ggattattcg ccgcaaagat
      901 gccgtgcttt ctccggcagc tgaacgcttt tttgccttgc tgattaattt gtggactgac
      961 gataaagaca atttgtggac caatatcgtc gaacgccaga gacatgcgtt acaggagata
     1021 ggttaataca ccggcgcagg agagactgcg ccggttaaat cagcggttag tgcgcgttga
     1081 ccactcccca cattggcccc tgcccaaccg catagcggcc tttttcatgc agtagcccct
     1141 gctcgccaac aatttcgtat accgagatgt ggtgagattt ttgcccggcg gcaatcagat
     1201 acttgccgct gtgatcaaca ttgaagccgc gcggctgggt ttccgttggc tggaagcctt
     1261 ctttactcaa cacgctgcca tcttccgaaa cgctgaaaac ggtaatcaga ctggcggtac
     1321 ggtcgcaggc gtataaatga cgaccatccg gggtgatatg aatatcagcc gcccaacggg
     1381 tgtcggagaa gttttccggc atcatatcca gcgtctggac acattcgata ttgccgtgcg
     1441 gatctttcag ttcccagaca tccactgagc tgtttaactc attgacgcaa tacgcatatt
     1501 gttcgtttgg atggaatacc atatgacgcg ggccggcccc ttcaacggta gtcacttccg
     1561 cagggtcctg cgctaccaga tgaccatcat cgctgaccgt aaacaggcaa atgcgatcct
     1621 gctttaatgc cggaacccac aacgtacggt tgtccggtga gatattggcg gaatggcaac
     1681 cgtccagccc ctcgaccaca tcgacgacgc ccactggcag gccatcttcc agacgcgtta
     1741 cgctcacgtt acccgcatta taagaaccga caaagacaaa ctgccccagg tgatcggtgg
     1801 aaatatgcgt cgggctcccc ggcagcgcag actctgcagc aaaggtcagc gcgccatcgt
     1861 ccggggcgat acgatacgcc aggacgcgaa actcagggcg aacaccaaca tagagatagc
     1921 gtttgtccgg gctgaccacc atcggctgca cctgccccgg cacatcgaca acctgtgtca
     1981 gcgtcagtgc gccttcatga ttcaggttcc agacgtgaat ttgctggctc tcagggctgg
     2041 cgatataaac tgtttgcttc atgaatgctc ctttgcattt agcaactgac tgtaacagct
     2101 aaaattagtc gcttttggcg gtgaatgctg aattaaaacg cagaattttg tccgatagtg
     2161 gtatcggtgt accattcgcg cagacgaaaa ttcttaacct taatctggaa tacgccatga
     2221 ccacacgcgt gattgctctc gacttagacg gcaccttatt gaccccgaaa aagaccctgc
     2281 ttccttcatc gatagaagcc ctggcccgcg ctcgcgaagc aggctatcga ttaatcatcg
     2341 tcacaggtcg ccatcacgtc gctattcatc ctttttatca ggcgctggcg ctggatacac
     2401 ctgctatttg ctgtaatggc acctatttgt atgattatca tgcaaaaacc gtgctggaag
     2461 cggacccaat gcccgttaat aaagccctgc aactcattga gatgctgaat gagcacaaca
     2521 ttcacggtct gatgtatgtg gatgatgcga tggtttatga gcacccgacc ggacatgtca
     2581 ttcgcacgtc taactgggcg caaaccctgc cgccggaaca gcgtccgact ttcacacaag
     2641 tcgcttctct ggctgaaacg gcgcaacaag ttaacgccgt atggaagttc gccctgacgc
     2701 acgatgacct gccgcaattg cagaattttg gtaagcatgt cgaacatgaa ctgggactgg
     2761 agtgtgaatg gtcctggcac gatcaggttg atattgcacg cggcggcaac agcaaaggta
     2821 aacgtttgac gaaatgggtt gaggcgcaag gctggtcgat ggaaaacgtc gtggccttcg
     2881 gcgataactt caatgatatt agtatgctgg aagccgctgg tacaggcgtg gcgatgggca
     2941 acgccgatga cgcagtaaaa gcgcgcgcca acattgtgat tggtgataac accaccgaca
     3001 gcattgccca gttcatttat agccacctga tttaatcagg cggttatcga cacactttta
     3061 atttgcgcat acagccacag gccaggtttg atcgccagtt catccctggc ccacgggctg
     3121 atacgcgccc acagcgtttt accgccgact tccagctcca cttccacctg gccgttgtcg
     3181 tcataactat taacaacttt tgcccgcaat acgttacgaa tgctggtttg ctgtggcggt
     3241 tgtaaaacca gcgagacatc ggaagcctga atgcggatgc gtagcgcagc ttgcagcggt
     3301 tcgtccagct tattgaccca caaatgctga tcgcccagcg ccagcgcggt catcgcgtaa
     3361 tgcggatgat gctcaagtac tgtcactttc agaatgctac tttgttgctc tttcggtagc
     3421 cacggattca tcacgctact accccagact tcttccagcg cgccaaaggc tttcacctga
     3481 ccgttttcca gtaccatcac tctgtctgcc agatggagga tctcatccag cgaatggctg
     3541 acatacaaca tcggaatgtt gatttcccgc gtcagacgtt gcagataagg caacagttcg
     3601 cgtttacgcg gaatatccag tgacgccagc ggttcatcca gcaacagcaa ttccggtgct
     3661 gtcagcaaag cccgaccaat cgccacgcgc tgtttttcgc ctccggacag gctgcctggt
     3721 aaacgatcaa gcaacggttc aatgcctaaa agcgccacca gcttatcgaa ctgattgacc
     3781 atgcttttcg ccatgccgta gcgcaaattg ccacgcactt tataatgcgg aaacagccgc
     3841 gcatcctgaa acacatagcc tacgcgacgc ttttctggcg tcaggcagat acctttatcg
     3901 gcgtcattta gtacccgacc attgaggaca atccgccctt tttgcggacg tgtcagccca
     3961 ctgatggcgt taatcagcga ggtttttccg gcaccagaga caccaaaaat agcggtgatg
     4021 ccattggcgg gcagcgtttc attaatcgtc aggcaatggt tgcccaacgt ctgggaaaaa
     4081 ttcagttcca gcatgattag cgccccgccc gctcacggct gattctggcc agccattctg
     4141 aaatcaacag ggagatcatc gccagcgcaa tagaaataat acacagtctc gccgcgccac
     4201 tttccccgcc aggggtctgg atcagggtat acatggcaga aggaatggtc cgcgtttcac
     4261 caggaatgtt cgacacaaag gtgatggttg cgccaaattc accgagcgaa cgagcgaatg
     4321 ccagtaccgt accaacaata attcccggca acgtcagcgg taaggtaata gtgaagaaga
     4381 cacgccagcg cccggcgccc aacgttcttg cggcctgctc cagtttgata tcaacacctt
     4441 ccagcgccag acgaatcgcc cgcaccatca gcggaaaaga catgacagcg gcagcgagaa
     4501 ccgcaccacg ccagctaaac gcgaaagaaa gaccaaacca gtcatacaga cgttcaccga
     4561 taaatccgcg ccgtcccatc gaaactaata ataggtaacc gacgaccacg ggcggtaaca
     4621 ccagcggtag atgcagtacg ctgtcgagca gagctttgcc cggaaacgtg caacgtacca
     4681 gtaaccaggc aaaaaagatc ccaaacggca ggctaaacag cacagccagg gaagaaactt
     4741 tcaggcttaa taaaactgcc tgccattctg gatcggtcag tatcattact tggttgtaaa
     4801 tccgtaacgt ttaaagattt ccgcagcctg cggccccttc agataatcgt aaaatgcttt
     4861 cactgtcgca ttgttatgcc cttccacaac agcaaccgga tattccactt ttttatgtga
     4921 atcttccggg aaggtggcaa ccacttttac ccctttgctg gcaactgcgt cagaaccgta
     4981 aacaataccc agcggcgctt cattgcgttc gaccaacgcc agcgcgccac gaacatcttc
     5041 cgccggagcc agtttcgggg agagcgtatc ccatgcgccc agtttttgca gtgcttcttt
     5101 cgcataaatg ccagcaggga catgttccgg atcaccaacc gcgaggcgac cgccattcag
     5161 cagcgaagtc cagttggttt tgctgtcgat ggtgaaatct ttctgctcgc tggctttcgg
     5221 tgctacaacg accaggctat tgccgagcag tgtctggcgc gaagccgtat cgatcgcttt
     5281 tttatcaacc gcataatcca tccatttctg atcggcagaa ataaacagat ccgcaggcgc
     5341 acccgcttca atctgacggg ccaaagtaga tgacgaagcg aaagaagaaa ccacatccac
     5401 gcctttctct tttttatact gcgtagcaat gtcctgcatt gcgttagtca gtgatgcggc
     5461 ggcgaacacc gtgattttcc cttcatctgc cagtgcattg ccagcaacag cgaaagagag
     5521 tgccgccccg gtaaacaagt tcaaccattt acgagccatt ggtaacccct taatctaacg
     5581 ttatgtaggc gtcaatataa cggcaagttc ggaattttcc cagcagttat tgctaacctt
     5641 tgtgaggtag atgagaaaaa atatcggcag aaaaagcagg aagttgagaa aaagaaaatg
     5701 cccgactaag cgggcattca gggaatcaat gattttgtcc gggctggtct tttttaccaa
     5761 caccagaaaa gatgttgaat acttcaccaa gaccgtaaat cagacccagg atgatggcca
     5821 tcacgacagg taccatgatt acggcgaata ccagactttt taataactct aacatggtca
     5881 actccagata tagtcatgag actattctaa ccgctaagca cagaaaagca ctcccctttt
     5941 gtgcggtcag ctttgcgtgg cgttcgtttt ccgtcacaat aagacttttg ccaggacatt
     6001 gttatgcagg ccgaaatcct tctcaccctt aagctccaac aaaaattatt cgccgacccg
     6061 cgccgcattt cgctactaaa acacattgcg ctttccggtt ccattagcca gggagcgaaa
     6121 gatgccggta tcagctataa aagcgcctgg gatgccatta acgagatgaa tcagttaagt
     6181 gagcatattc tggttgagcg cgcaacaggc ggtaaaggtg gaggcggcgc agtactgacc
     6241 cgctatggtc agcgactgat tcagctctat gacttactgg cgcaaatcca gcaaaaagcc
     6301 tttgatgtgt taagtgacga tgacgccctg ccgctgaaca gcctgctggc cgcgatctca
     6361 cgtttttcac tgcaaaccag cgcccgtaac cagtggttcg gtaccatcac cgcccgcgat
     6421 catgatgacg ttcaacagca tgttgatgtc ttactggctg atggaaaaac acgcctgaaa
     6481 gtcgcagtta ccgcacaaag cggcgcgcgt ctggggctgg atgaaggcaa agaagtgttg
     6541 atattgctaa aagcgccgtg ggtaggtatt actcaggacg atgcggtcgc gcaaaacgct
     6601 gacaaccaat taccgggtat tattagtcat attgagcgcg gcgcagagca gtgcgaagta
     6661 ttaatggcgc tacccgacgg gcaaacactg tgcgccacag tgccggtaaa tgaagcgact
     6721 tctcttcagc aaggacagaa tgtcacggcc tactttaatg ccgacagcgt gattatcgcc
     6781 acgctgtgct aagcgtgttg acaatttgtt atgaaacacg tatccctgtc agtaatcgct
     6841 gcacaaaatg ggatataaaa tgtcatcgtt gcaaattttg caaggcacgt ttcgtcttgg
     6901 cgacacaaaa acgctgcaat tgcctcggct aacgttaaac gcgggtgata gttgggcgtt
     6961 tgtcggttcg aatggaagcg ggaaatcggc gctggcccgc gcgctgtcgg gggaacttcc
     7021 gcttttgaaa ggtgaacggc aaagccagtt ttcccacatc actcgtctct ccttcgagca
     7081 attgcaaaag ctcgtcagcg acgaatggca gcggaataac accgatatgc tcggccctgg
     7141 cgaagatgac accgggcgta ccacggctga gatcattcag gatgaagtaa aggatactcc
     7201 gcgttgcatg caactggcgc agcaattcgg tattaccgcc ctcctcaacc gacgctttaa
     7261 atacctttcc actggcgaga cgcgaaaaac cctgctgtgt caggcgctga tgtcggagcc
     7321 tgacttgttg atccttgatg aaccgttcga tggcctggat gttgcctcac gtcagcagct
     7381 ggctgagcta ctcgcctcgt tacatcagtc cggtattact ctggtactgg tgctcaatcg
     7441 cttcgatgag atcccggaat ttgtccagtt tgctggcgtg ctggcggatt gcaccttagc
     7501 ggaaactggc gctaaagaag aactgctcca acaagcactc gttgcgcaac tggcgcatag
     7561 cgaactgctt gaaggtgtgc aaatgccgga gccggatgaa ccttcagtac gtcacgcctt
     7621 acccgcccac gaaccgcgca ttgtgctgaa caatggcgtg gtttcttata acgaacgccc
     7681 catccttaat aaccttagct ggcaggtgaa tccaggcgaa cactggcaaa ttgtcgggcc
     7741 aaatggcgcg ggaaaatcga cgttattaag cctgattact ggcgatcatc cgcaaggtta
     7801 cagcaacgat ttgacgcttt tcggacgacg tcgcggcagc ggcgaaacca tctgggatat
     7861 caaaaagcat atcggttacg tcagcagtag tttgcatctg gattaccggg tcagcactac
     7921 tgtgcgtaat gtgattcttt ctggctattt tgattcgatt ggcatttatc aggccgtttc
     7981 ggatcgccag caaaaactgg tgcagcagtg gctggatatt ctcggcattg ataaacgcac
     8041 ggctgacgct cctttccata gtctttcctg gggacagcag cgtctggcgc tgatcgtccg
     8101 tgcgctagtg aaacatccga cgttgcttat tctcgatgaa ccactacagg ggcttgatcc
     8161 gctcaatcgc cagcttatcc gccgttttgt tgatgtgctg attagcgaag gtgaaacgca
     8221 attgttgttt gtttcgcacc acgctgaaga tgcgcctgcc tgtattaccc atcgtcttga
     8281 gttcgtgccg gacggtgact tttatcgcta tgcgctgaca aaaataaact gagtcggtag
     8341 tgctgaccgt gccggaggcg gcgtaaacgt gttatccggc caacggtttg acgcatgcag
     8401 gcatgaaacc gcgtcttttt ttcagataaa aagcgcaatc attcataaac cctctgtttt
     8461 ataatcactt aatcgcgcat aaaaaacggc taaattcttg tgtaaacgat tccactaatt
     8521 tattccatgt cacacttttc gcatctttgt tatgctatgg ttatttcata ccataagcct
     8581 aatggagcga attatgagag ttctggttac cggtggtagc ggttacattg gaagtcatac
     8641 ctgtgtgcaa ttactgcaaa acggtcatga tgtcatcatt cttgataacc tctgtaacag
     8701 taagcgcagc gtactgcctg ttatcgagcg tttaggcggc aaacatccaa cgtttgttga
     8761 aggcgatatc cgtaacgaag cgttgatgac cgagatcctg cacgatcacg ctatcgacac
     8821 cgtgatccac ttcgccgggc tgaaagccgt tggcgaatcg gtacaaaaac cgctggaata
     8881 ttacgacaac aatgtcaacg gcaccctgcg cctgattagc gccatgcgcg ccgctaacgt
     8941 caaaaacttt atctttagct cctccgccac cgtttatggc gatcaaccca aaattccata
     9001 tgttgaaagc ttcccgaccg gcacaccgca aagcccttac ggcaaaagca agctgatggt
     9061 ggaacagatc ctcaccgatc tgcaaaaagc ccagccggac tggagcattg ccctgctgcg
     9121 ctacttcaac ccagttggcg cgcatccgtc gggcgatatg ggcgaagatc cgcaaggcat
     9181 tccgaataac ctgatgccat acatcgccca ggttgctgta ggccgtcgcg actcgctggc
     9241 gatttttggt aacgattatc cgaccgaaga cggtactggc gtgcgcgatt acatccacgt
     9301 aatggatctg gcggacggtc acgtcgtggc gatggaaaaa ctggcgaaca agccaggcgt
     9361 acacatctac aacctcggcg ctggcgtggg cagcagcgtg ctggatgtgg ttaatgcctt
     9421 cagcaaagct tgcggcaaac cggttaacta tcattttgca ccgcgtcgcg agggcgacct
     9481 tccggcctac tgggcggacg ccagcaaagc cgaccgtgaa ctgaactggc gcgtaacgcg
     9541 cacactcgat gaaatggcgc aggacacctg gcactggcag tcacgccatc cacagggata
     9601 tcccgattaa ggaacgacca tgacgcaatt taatcccgtt gatcatccac atcgccgcta
     9661 caacccgctc accgggcaat ggattctggt ttcaccgcac cgcgctaagc gcccctggca
     9721 gggggcgcag gaaacgccag ccaaacaggt gttacctgcg cacgatccag attgcttcct
     9781 ctgcgcaggt aatgtgcggg tgacaggcga taaaaacccc gattacaccg gcacttacgt
     9841 tttcactaat gactttgcgg ccttgatgtc tgacacgcca gatgcgccag aaagcaacga
     9901 tccgctaatg cgttgccaga gcgcgcgcgg taccagccgg gtgatctgct tttcaccgga
     9961 tcacagtaaa acgttgccag aactgagcgt tgcggcattg acggaaatcg tcaaaacctg
    10021 gcaggagcaa accgcagaac tgggggaaac gtacccatgg gtgcaggtct ttgaaaacaa
    10081 aggcgcggcg atgggctgct ctaacccgca tccgcacgga cagatttggg caaatagctt
    10141 cctgcctaac gaagctgagc gcgaagaccg cctgcaaaaa gaatattttg ccgaacagaa
    10201 atcaccaatg ctggtggatt atgttcagcg cgagctggca gacggtagcc gtaccgttgt
    10261 cgaaaccgaa cactggttag ccgtcgtgcc ttactgggct gcctggccgt tcgaaacgct
    10321 actgctgccc aaagcccacg ttttacggat caccgatttg accgacgccc agcgcagcga
    10381 tttggctctg gcgttgaaaa agctgaccag tcgttatgac aacctcttcc agtgctcctt
    10441 cccctactct atgggctggc acggcgcgcc atttaatggc gaagataatc aacactggca
    10501 gctgcacgcg cacttttatc cgcctctgtt gcgctccgcc accgtacgta aatttatggt
    10561 tggttatgaa atgctggcag aaacccagcg agacctgacc gcagaacagg cagcagagcg
    10621 tttgcgcgca gtcagcgata tccattttcg cgaatccgga gtgtaagaaa tgagtctgaa
    10681 agaaaaaaca caatctctgt ttgccaacgc atttggctac cctgccactc acaccattca
    10741 ggcgcctggc cgcgtgaatt tgattggtga acacaccgac tacaacgacg gtttcgttct
    10801 gccctgcgcg attgattatc aaaccgtgat cagctgtgcg ccacgcgatg accgtaaagt
    10861 tcgcgtaatg gcagccgatt atgaaaatca gctcgacgag ttttccctcg atgcgcccat
    10921 tgtcgcgcat gaaaactatc aatgggcgaa ctacgttcgt ggcgtggtga aacatctgca
    10981 actgcgtaac aacagcttcg gcggtgtgga catggtgatc agcggcaatg tgccgcaggg
    11041 tgcagggtta agttcttccg cttcactgga agtcgcggtc ggaaccgtat tgcagcagct
    11101 ttatcatctg ccgctggacg gcgcacaaat cgcgcttaac ggtcaggaag cagaaaacca
    11161 gtttgttggc tgtaactgcg ggatcatgga tcagctaatt tccgcactcg gcaagaaaga
    11221 tcatgccttg ctgattgact gccgctcact ggggaccaaa gcagtttcca tgcccaaagg
    11281 tgtggctgtc gtcattatca acagtaactt caaacgtacc ctggttggca gcgaatacaa
    11341 cacccgtcgt gaacagtgcg aaaccggtgc gcgtttcttc cagcagccag ccctgcgcga
    11401 tgtcaccatt gaagagttca acgctgttgc gcatgaactg gacccaatcg tggcgaaacg
    11461 cgtgcgtcat atcctgactg aaaacgcccg caccgttgaa gctgccagcg cactggagca
    11521 aggcgacctg aaacgtatgg gcgagttgat ggcggagtct catgcctcta tgcgcgatga
    11581 tttcgaaatc accgtaccgc aaattgacac tctggtagaa atcgtcaaag ctgtgattgg
    11641 cgacaaaggt ggcgtacgca tgaccggcgg cggatttggt ggctgtatcg tcgcgttgtt
    11701 cccggaagag ctggtgcctg ccgtacagca agctgtcgct gaacaatatg aagcaaaaac
    11761 aggtattaaa gagacttttt acgtttgtaa accatcacaa ggagcaggac agtgctgaac
    11821 gaaactcccg cactggcacc cgatggtcag ccgtaccgac tgttaacttt gcgtaacaac
    11881 gcagggatgg tagtcacgct gatggactgg ggtgcgactt tactttccgc ccgtattccg
    11941 ctttccgatg gcagcgtccg cgaggcgctg cttggctgcg ccagcccgga atgctatcag
    12001 gatcaggccg catttctggg ggcctctatt ggtcgttatg ccaaccgcat cgccaatagc
    12061 cgttatacct ttgacggtga aaccgtgacg ctttcaccaa gtcagggcgt taaccagctg
    12121 cacggcggcc cggaagggtt cgacaaacgt cgctggcaga ttgtgaacca gaacgatcgt
    12181 caggtgctgt ttgccctgag ttcagatgat ggcgatcagg gtttcccggg taatctcggc
    12241 gcgacggtgc aatatcgtct gaccgacgat aaccgtatct ccattactta tcgcgccaca
    12301 gttgataaac cttgcccggt gaatatgact aatcacgtct atttcaatct tgacggcgag
    12361 cagtctgacg tgcgcaatca caagttgcag attctggcgg aagaatatct gccggttgat
    12421 gaaggcggca ttccgcacga cggcctgaaa tctgtcgccg gaacctcttt tgatttccgc
    12481 aacgccaaaa tcatcgccag tgagtttctt gccgacgacg atcagcgcaa agtgaaaggt
    12541 tacgatcacg cgttcttgtt acaggccaaa ggcgatggca agaaagtggc ggcgcatgtc
    12601 tggtcagcag atgaaaaatt gcagctgaag gtctacacca ccgctccggc tctgcaattc
    12661 tactccggca acttcctcgg cggcacaccg tcgcggggaa ccgaacctta cgccgactgg
    12721 caagggctgg ctctggaaag cgaatttctg ccggacagcc cgaaccaccc tgaatggccg
    12781 caaccggact gcttcctgcg tcctggcgaa gagtattcca gcctgacgga atatcagttt
    12841 attgctcagt aatgttaagc cctcgtcatg agggctttat cttcatattg ttcaaatcac
    12901 cagcaaacac cgacatattt gcaactcaat attcacaaca accttacact gcgacactat
    12961 tttcgctatg gttatgcgta agcattgctg ttgcttcgtc acggcaatat aatgagaatt
    13021 attatcatta aaagatgatt tgaggagtaa gtatatggct gtaactaagc tggttctggt
    13081 tcgtcatggc gaaagtcagt ggaacaaaga aaaccgtttc accggttggt acgacgtgga
    13141 tctgtctgag aaaggcgtaa gcgaagcaaa agcagcaggt aagctgctga aagaggaagg
    13201 ttacagcttt gactttgctt acacctctgt gctgaaacgc gctattcata ccctgtggaa
    13261 tgtgctggac gaactggatc aggcatggct gcccgttgag aaatcctgga aactgaacga
    13321 acgtcactac ggtgcgttgc agggtctgaa caaagcggaa accgctgaaa agtatggcga
    13381 cgagcaggtg aaacagtggc gtcgtggttt tgcggtgact ccgccggaac tgactaaaga
    13441 tgatgagcgt tatccggggc acgatccgcg ttacgcgaaa ctgagcgaga aagagctgcc
    13501 gctgacggaa agcctggcgc tgaccattga ccgcgtgatc ccttactgga atgaaaccat
    13561 tctgccacgt atgaagagcg gtgagcgcgt catcatcgcc gcacacggta actcgttacg
    13621 tgcgctggtg aaatatctcg ataacatgag cgaagaagag attcttgagc tgaatatccc
    13681 gaccggcgtg ccgctggttt atgagtttga cgagaacttc aaaccgctga aacgctatta
    13741 tctgggtaat gctgacgaga tcgcggcgaa agcagcggcg gttgctaacc agggtaaagc
    13801 gaagtaaacg tcattcgttt aaaaatgaga aagccgactg caagtgagtc ggcttttttg
    13861 tttgtcgttg ctaacaatgg agcacattgc ctgatgcgac gctgcgcgtt ttatcaggcc
    13921 tccggtgatt aatcggatac gccactctga cggcgcatcc gacaattaaa ctttacccgc
    13981 gacgcgcttt tactgcattc gccagttgac gtaacagagc atcggtatca tcccagccaa
    14041 tgcaggcatc ggtgatgctc ttgccatagg ccagcggttc cccactctcg aggctctgat
    14101 tgccttccac cagatggctt tccaccatca cgccaataat ggccttttcg ccaccggcaa
    14161 tctgctggca aacgtcagta caaacatcca tctgcttttt gaattgtttt gacgagttag
    14221 catggctgaa atcgatcatc acctgtgctg gcaggcctgc tttgttcagc ccttctttta
    14281 cttcagcaac gtgcttcgcg ctgtagttag gctctttacc gccgcgcaga atgatatggc
    14341 aatcgccgtt accgctggta ttcacaatcg ccgaatgccc ccatttcgtt acggacagga
    14401 agcagtgcgg cgcaccggcg gcattaatgg catcgatagc cactttaatc gtaccgtcgg
    14461 tgccattttt gaaacctacc ggacaagaaa gaccagacgc cagttcgcgg tgcacctgcg
    14521 attcggtggt acgtgcacca attgcgcccc agctcatcag gtcagcgaga tattgtgggg
    14581 tgatcatatc gagaaactca cccgccgctg gcagaccgct gtcgttaata tcaagcagca
    14641 atttacgggc aatacgcaga ccgtcgttga tctggaagct gttatccata tgcggatcgt
    14701 taatcagccc tttccagccc accgtggtac gcggcttttc aaaatagacg cgcattacga
    14761 tttccagctc atctttcagc tcttcacgca gcgccagcaa gcgagtggca tactctttag
    14821 ccgcgacagg atcatgaatt gagcatgggc caatcaccac caacaggcga tcatcattac
    14881 ctttcaggat cttatggatc gcttttcggg catgggcgac cgtattcgcg gcattttcag
    14941 tagcggggaa tttttccagc aatgcgacag gaggaagtaa ctctttgatt tctttgatgc
    15001 gtaaatcgtc gttctgataa ttcatgtctg ttccagtgtt gccatactta tcttagtgaa
    15061 tgcaatactt ccaatctata tcttccgtca gaatgtgtaa acggggtttt acactatgaa
    15121 cggattaatc ctggaattag caagaaaaac gccagattgt cgcgaaaaac gagatctctc
    15181 ctacaatttc taactgtaac tcctttgttt gttaattatt tcaagattct ctgctgggta
    15241 tctttgcctg gctgaaatct taaaccattg ccttatattc acctgcaaat gcactgttgg
    15301 aagaggttat ccgacataac gaccataaca ggagcatcct atgaaaatga caaaactggc
    15361 cacactattt ttgactgcca ctctaagcct tgccagcggt gccgcactgg ctgccgatag
    15421 cggagcgcaa agcaataacg gccaggcaaa cgccgcagct gatgcgggcc aggtagcccc
    15481 tgatgcccgt gaaaatgtcg cgccaaataa cgtcgacaat aacggggtaa ataccggttc
    15541 tggcggcaca atgctgcatc cggatggttc ttcaatgaac aatgacggca tgaccaaaga
    15601 tgaagagcac aagaacacca tgtgcaaaga tggtcgctgc ccggatatca ataaaaaagt
    15661 ccaaaccggt gatggcatca acaatgatgt cgataccaaa accgacggta ccacgcagta
    15721 accctgcgaa atagccaata atgggagagc ggagtctctc ccttttaatt tttcccttct
    15781 aaaagcagta tgatgtcata aagttaagtg agaataatta agggatgact cgtcatggcg
    15841 cactcacact cacacacttc ctcacacctg ccagaagata ataatgctcg tcgcttgttg
    15901 tatgctttcg gcgtgactgc cgggtttatg ctcgtagaag tcattggcgg ttttctttcc
    15961 ggttctctgg cattgctggc cgatgcgggt catatgttga ccgatactgc cgccctgctt
    16021 ttcgccctac tcgcagtgca attttcccgt cgtcctccca ccattcgcca cactttcggt
    16081 tggctaagat tgaccacgct cgcggcgttt gtgaatgcca tcgccctggt ggtgattact
    16141 attttgattg tctgggaggc gatagaacgc ttccgcactc cgcgtccggt cgagggcggc
    16201 atgatgatgg caattgccgt ggctgggctg ctggcaaata tactttcttt ctggttactt
    16261 catcacggca gcgaagagaa aaacctcaac gtgcgagcag cggcactgca tgtgctcggg
    16321 gacctgctgg gttcggttgg ggctattatt gccgcgttga ttattatctg gaccggctgg
    16381 acacctgctg accccattct ctcaatactg gtgtcgcttc tggttctgcg tagcgcgtgg
    16441 cgactgttga aagatagcgt gaatgaatta cttgaaggtg caccggtatc gctggatatc
    16501 gccgaactga agcgtcgtat gtgccgggaa atcccggaag tccgcaatgt gcaccatgta
    16561 catgtatgga tggtaggcga gaagccggtg atgacgctgc atgtgcaggt gatcccgcca
    16621 cacgatcacg atgccttgtt agatcagatc caacactacc tgatggatca ctatcagatt
    16681 gagcacgcca ctattcagat ggaatatcaa ccttgtcatg ggccagactg ccacctgaat
    16741 gaaggggtgt ctggccattc acatcatcac cattaatggg acagcgcgcg tgaacctctt
    16801 tcgcgtgcgc tgttgatcca catccggctg ccgttgaggg caataaaggt caggatgatg
    16861 tactccagag acatggcgta aacgccctga agtgcaaaaa taacgacgct aatcacgtta
    16921 ataatcaccc acaacagcca gttttccaca tacttacgcg tcatcagaat cattgccacg
    16981 atagataaca ccatcatgca tgaatcccag aacgggaaag cgtccggctg tagttcaggc
    17041 atcgcaacct gtaatcctaa tgcttgcatg atcatgactg ccacgcgggt caaaaatgca
    17101 aacaccggat tgataaatac cgtcatcaga ccaatcgaaa caacgcaaac cgccaaccag
    17161 cttagcgcct tcggcaatgg caaccagcga attttcaact ccgcctcgtt ctgactggtt
    17221 tgtcgcgacc acgcatacca gccgtaaata ttcgcggcaa agaaaaacac ctgtaatagc
    17281 aggctggcat acagctgaat ctgaaagaaa ataatgccaa acaaggtgac gttgatcagg
    17341 ccaaagaagt agttactgat cttctccaga ctggcaagac caatacacag caacccggcg
    17401 atcgtgccta ccgcttcgat ccatgagaga tcataaccgc ctgcccctat tggtatatgt
    17461 accaggatat tctgcacact aaaaaaatcc atcgttcccc caaagcgtaa tgtgacgcct
    17521 tattatcaat tatccacgta gtgtagccgc aaaatccagc atacgattga gcgacaccag
    17581 ggccctctct cgcagccttt catcaacatg aacctcgtga ttgcttcctt cctgttctaa
    17641 tgcctctgcg atggcctgaa ggccattcat ggccatccac gggcaatgcg cgcagctgcg
    17701 acaggttgcg ccctcacccg cggttggtgc ttccagtaac tctttatctg gcaccgcctg
    17761 ctgcattttg tagaaaatgc cccgatcggt tgccacgata agccgctgat gtggcaatgt
    17821 tttcgcagca gcgatcagtt gactggtgga accgaccgca tccgccatct ctacaatagc
    17881 ttgcggtgat tctggatgca ccagtatggc agcatccggg tattcttctt gcaagcgggt
    17941 taacgcctga gtcttaaact catcatgcac aatgcaggca ccctgccagc ataaaatgtc
    18001 tgcgcccgtc tgtttttgca cgtaacgccc cagatgtttg tcaggtgccc agatgatttt
    18061 ttcacccaaa ctatccagat gatcaataag ttcgacggca atgcttgaag ttaccaccca
    18121 atctgcgcgc gcttttaccg cagcagaagt gttggcgtag acgacgacag tacgatcggg
    18181 atgggcatcg caaaatgcgt taaattcttc aacagggcag ccgagatcca gtgaacattc
    18241 agcctgaagt gtcggcatca gaattgtttt ttccggactg agaattttgg cggtttctcc
    18301 cataaatctc accccagcga ctaacaacgt agaagcggga tgctttgcac cgaagcgcgc
    18361 catttccaga gagtcagaaa tacagccacc ggtttcttct gccagttgtt gaatttcggg
    18421 atcggtatag tagtgggcaa ccatcaccgc attacgttct tttagtagac gttttatctt
    18481 ctcgcggtaa tacgcttttt catcaatgct taacggcgtc ggcttcgggg ggaaaggata
    18541 aatcgccgtg tctggatcaa acatcacgct catcttacca tctcgttcta caggcttaac
    18601 gttaaaaccg acttaagcgt gatattcgct attatcggcg taatttgttt tgtatactaa
    18661 acaagatagt cgaatggtgg ggaaaagtca ctcgaattac gtatgagatg ccgaatgcca
    18721 cactttgcgt cttattcctc cgacgtagag ttgtttacgc agcaaacgcg gagtatgtca
    18781 tcagggtcaa gaattgaaat tgagagatgc ctgaatagca aaaaagcgcc tttagggcgc
    18841 ttttttgcat tggtgggtcg tgcaggattc gaacctgcga ccaattgatt aaaagtcaac
    18901 tgctctacca actgagctaa cgaccccttg cgggattatg ctgctttaat cattcaggat
    18961 ggtgggtcgt gcaggattcg aacctgcgac caattgatta aaagtcaact gctctaccaa
    19021 ctgagctaac gacccatacg ggtgttacct gaaagatttc tactcggaac caatgtttaa
    19081 aattggtggg tcgtgcagga ttcgaacctg cgaccaattg attaaaagtc aactgctcta
    19141 ccaactgagc taacgacccg agtggtgggt gatgacggga tcgaaccgcc gaccccctcc
    19201 ttgtaaggga ggtgctctcc cagctgagct aatcacccga tactgcgctg gatactacaa
    19261 actacttcca ccgaacccac cgtcattagt ggtgggtcgt gcaggattcg aacctgcgac
    19321 caattgatta aaagtcaact gctctaccaa ctgagctaac gacccggtgg tgggtgatga
    19381 cgggatcgaa ccgccgaccc cctccttgta agggaggtgc tctcccagct gagctaatca
    19441 cccgatacta cgctggatac tacaactaac tatgttagtg gtgggtcgtg caggattcga
    19501 acctgcgacc aattgattaa aagtcaactg ctctaccaac tgagctaacg acccactttt
    19561 tcgttgcttc ggtttgtttg atatcccgtg gcaacggcgg catatattac tgatttcaga
    19621 cttgagcgca acaaaaattt cgatgtagat cactcaactg cttatgattc gcacgacacg
    19681 accagaaata atgcgacttc tggtcgtgtg ttatgcatta catcgcgttc agacgttttt
    19741 gcgcctgttt agcgccatca gtaccagggt atttactgat aacctgctgg tatacggctt
    19801 tcgcttttgc ggtgtcacct ttgtcctgca tgatgacgcc gactttaaac atcgcatctg
    19861 cagccttcgg tgacttcgga tagtttttca ctaccgaagc aaaataatac gccgcatcat
    19921 cttttttacc tttgttgtag tttaactgac ccagccaata attggcgttt ggcaggtaag
    19981 ttgaatcagg gtaatttttg atgaaattct gaaatgccac cattgcgtca tcctggcggg
    20041 atttatcctg caccagcgca atagctgcat tgtaatccgt gtttgcatca ccgcttttca
    20101 ccggcgcgcc agcattcgca gtaccagcat cagctgtcgg cgtcgttgat gccgccacac
    20161 cgctttgatc gccgctggtt gattgcgccg ctgcaccacc gctgctgagg ctgtcgatct
    20221 gcaacaggat ctgcttttgc cgctccacga cctgattcag ttgatactga ttttcctgaa
    20281 tctgaccacg cagggaatca atatcggatt gattatcaga gagttgttgc tggagttggg
    20341 ttaaaagctg gctgtgagca ttagaaatac gctcaagttg agtgacgcgg tcttcgaccg
    20401 agcctgagcc gacactactg attggtgcct gagcaaaagc ggcccagggg gccgctatac
    20461 caaccagtaa cgacagactc aatagttgat gtctgaagtt actgctcatg caattctctt
    20521 agtaaaccag taccgcacga cggtttttgg catacgccgc ttcgtcatga cccagtactg
    20581 caggtttttc tttaccgtaa gaaacgatgg agatctggtc agcagaaacg cctttaccct
    20641 gcagatacat cttaacagcg ttcgcacgac gttcacccag ggagatgttg tattccggag
    20701 taccacgttc gtccgcgtga ccttctacgg tgactttgta agacgggttg ctacgcagga
    20761 agtttgcgtg tgcatccagc atttgagcga agtcagaacg gatatcgtac ttgtccagat
    20821 cgaagtaaac gatgttgttc tgctgcagct gttgcatttg cagacgagcc tgctcttcgg
    20881 aagacatgtt gccgttgccg ccgttcgcat ccataccagt gccggcaccc agcatgcctt
    20941 cgctgccgtc attgctggcg ttcttgttgg aagaacatgc cgcaattgcc ataacaggca
    21001 gagcaatcat cagccctttc agcactttgt tcagttgcat ttcaatgatt cctttactat
    21061 tcaattaatt attatcacag atacggcgac caggcaggga atttgacctg tccatcagtt
    21121 gccggaagac gcgctttgaa acgcccatct gtagaaacca aattcagcac ggatcccatc
    21181 ccctgagaag agctgtagat taccatagtg ccgttaggtg ccagacttgg cgtttcatcc
    21241 aggaacgtgg acgacagaac ttgtacgcct cccgttgcca gatcttgttt ggcaatgtgc
    21301 tgctgcccac cgttggagct gaccattacc ataaatttac cgtcgctgct gacatccgca
    21361 tcctggttct gcgaaccttc ccaggtaata cgttgtggcg caccgccgtt gatattcact
    21421 ttataaacct gcggacgacc ggcctggtca gaagtaaatg ccaggttctg gctgtccggg
    21481 aaccaggtcg gttcggtatt gttactgcga ccatcagtta cctggcggat ctgaccagaa
    21541 gccaaatcca ttacgtacag gttcaggcta ccggttttcg acaaggcgaa tgccagtttg
    21601 ctgccgtctg gcgagaatgc aggtgcaccg ttgtgacgcg ggaatgaagc cacctgacgt
    21661 acagcgccat ttgccagcgt ctgaataacc agcgcggaac gaccgctttc gaaggtcaca
    21721 taagccagtt tagaaccgtc tggtgaccac gccggagaca tcagcggttg cggtgaacgg
    21781 tgaacgacaa actggttgta accgtcatag tcagatacgc gcagttcata cgggaactga
    21841 ccgccgttag tctgaacaac gtaggcaata cgggtacgga acgcaccttt aataccggtc
    21901 agtttttcaa atacttcgtc actggcggta tgaccagcat aacgcagcca ctgcttgttc
    21961 actttgtacg agttctgagc aagtacagta cccggtgcgc cgccagtgtc aacaagttga
    22021 taagcaacat tgtaagaacc atccggattc ggggtgacct gaccgacaac cacagcgtca
    22081 atgcccagcg cggaccatgc agctggttgt acttcctgcg cactacccgg ctgctgtggc
    22141 agacgagcac gatctaacgg attaaattta ccgctgttac gcaagtcagc agcaacgatg
    22201 ccgccaatat cttcaggtgc cgcaccaggc cccgcccact ggaaaggaac aacaccaata
    22261 ggacgaccgg aatctacacc gctgtcgatc acaatgcgga cttcagcatg cagaactgat
    22321 gcccacagta tgagaaaacc aaatgctact cgtaatgcct gcttcatcat atctccctta
    22381 tctggaccga tggcccacga taatttagca gaatgttaac aaactaaaat acacaaaact
    22441 accagaaccc cgtggcaacc ggtgcctgat gttgaccgtc ggaacagtca acatcgcgat
    22501 tacggtttga agtccaatgg cgcgtttttg aacacttcat atactgcctg gcttggtggt
    22561 ttcgggatct tcgcaagttt agctgctgcc aacgcagctt gacaaagtgc gggatcgcca
    22621 ccttcaggtt tgatatccag taacatacca tcgggtgcca gttttatgcg cagcgtacag
    22681 gttttgcctg cataggacga tgcgtcatag aacttacttt cgatagcaga tttaatctgc
    22741 ccggcatagt tattgatatc ggcccctgat gcgccattgt ttttagtatt accactcccg
    22801 gcaggcgaag cattgttccc tttcgcccct ccccccgttt tcggtgcatt cttaccagag
    22861 cttagctcac cgaaaatatc atctgcctct gcggcagctt ttgctgcagc ggctttttca
    22921 gctgctgctt ttgccgctgc cgcttttttg tctgcggcgg ctttttctgc cgctgctttc
    22981 ttatcagctg c
//
LOCUS       DAZFJL010000077        22640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFJL010000077 DAZFJL010000000
VERSION     DAZFJL010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.077"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..1830)
                     /gene="flhA"
                     /locus_tag="ACHJ8C_002847"
     CDS             complement(<1..1830)
                     /gene="flhA"
                     /locus_tag="ACHJ8C_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO5552673.1"
                     /translation="MSNLAAMLRLPANLKATQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEAKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVYVIGLDTPLERLL"
     gene            complement(1823..2971)
                     /gene="flhB"
                     /locus_tag="ACHJ8C_002848"
     CDS             complement(1823..2971)
                     /gene="flhB"
                     /locus_tag="ACHJ8C_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO5552674.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVALARPLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     AIISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(3180..3824)
                     /gene="cheZ"
                     /locus_tag="ACHJ8C_002849"
     CDS             complement(3180..3824)
                     /gene="cheZ"
                     /locus_tag="ACHJ8C_002849"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO5552675.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(3835..4224)
                     /gene="cheY"
                     /locus_tag="ACHJ8C_002850"
     CDS             complement(3835..4224)
                     /gene="cheY"
                     /locus_tag="ACHJ8C_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO5552676.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGLDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(4239..5288)
                     /gene="cheB"
                     /locus_tag="ACHJ8C_002851"
     CDS             complement(4239..5288)
                     /gene="cheB"
                     /locus_tag="ACHJ8C_002851"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO5552677.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSVPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(5291..6151)
                     /gene="cheR"
                     /locus_tag="ACHJ8C_002852"
     CDS             complement(5291..6151)
                     /gene="cheR"
                     /locus_tag="ACHJ8C_002852"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO5552678.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRALGLADFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVSLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(6266..6396)
                     /locus_tag="ACHJ8C_002853"
                     /pseudo
     CDS             complement(6266..6396)
                     /locus_tag="ACHJ8C_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
     gene            complement(6442..8103)
                     /gene="tar"
                     /locus_tag="ACHJ8C_002854"
     CDS             complement(6442..8103)
                     /gene="tar"
                     /locus_tag="ACHJ8C_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO5552679.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(8248..8751)
                     /gene="cheW"
                     /locus_tag="ACHJ8C_002855"
     CDS             complement(8248..8751)
                     /gene="cheW"
                     /locus_tag="ACHJ8C_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO5552680.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(8772..10736)
                     /gene="cheA"
                     /locus_tag="ACHJ8C_002856"
     CDS             complement(8772..10736)
                     /gene="cheA"
                     /locus_tag="ACHJ8C_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO5552681.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAREVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVDVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDLLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQRGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(10741..11667)
                     /gene="motB"
                     /locus_tag="ACHJ8C_002857"
     CDS             complement(10741..11667)
                     /gene="motB"
                     /locus_tag="ACHJ8C_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO5552682.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKHAEQAILHE
                     NAESQNEPVSALEKTEVAPQVSVPTMPSAEPR"
     gene            complement(11664..12551)
                     /gene="motA"
                     /locus_tag="ACHJ8C_002858"
     CDS             complement(11664..12551)
                     /gene="motA"
                     /locus_tag="ACHJ8C_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO5552683.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLEFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(12678..13256)
                     /gene="flhC"
                     /locus_tag="ACHJ8C_002859"
     CDS             complement(12678..13256)
                     /gene="flhC"
                     /locus_tag="ACHJ8C_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO5552684.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(13259..13609)
                     /gene="flhD"
                     /locus_tag="ACHJ8C_002860"
     CDS             complement(13259..13609)
                     /gene="flhD"
                     /locus_tag="ACHJ8C_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO5552685.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            14389..14817
                     /gene="uspC"
                     /locus_tag="ACHJ8C_002861"
     CDS             14389..14817
                     /gene="uspC"
                     /locus_tag="ACHJ8C_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO5552686.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRNVMQEETQSFLDKLIQDARYPVDKTFIAYGELSEHILEVCRKHH
                     FDLVICGNHNHSFFSRASCSAKRVITSSEVDVLLVPLTGD"
     gene            complement(14824..16248)
                     /gene="otsA"
                     /locus_tag="ACHJ8C_002862"
     CDS             complement(14824..16248)
                     /gene="otsA"
                     /locus_tag="ACHJ8C_002862"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO5552687.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNKFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLGDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTACGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(16223..17023)
                     /gene="otsB"
                     /locus_tag="ACHJ8C_002863"
     CDS             complement(16223..17023)
                     /gene="otsB"
                     /locus_tag="ACHJ8C_002863"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO5552688.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDTILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPKGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(17190..18176)
                     /gene="araH"
                     /locus_tag="ACHJ8C_002864"
     CDS             complement(17190..18176)
                     /gene="araH"
                     /locus_tag="ACHJ8C_002864"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO5552689.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(18191..19705)
                     /gene="araG"
                     /locus_tag="ACHJ8C_002865"
     CDS             complement(18191..19705)
                     /gene="araG"
                     /locus_tag="ACHJ8C_002865"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO5552690.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQIKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAITAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(19775..20764)
                     /gene="araF"
                     /locus_tag="ACHJ8C_002866"
     CDS             complement(19775..20764)
                     /gene="araF"
                     /locus_tag="ACHJ8C_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO5552691.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKASDIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            21561..22064
                     /gene="ftnB"
                     /locus_tag="ACHJ8C_002867"
     CDS             21561..22064
                     /gene="ftnB"
                     /locus_tag="ACHJ8C_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO5552692.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(22142..22393)
                     /gene="yecJ"
                     /locus_tag="ACHJ8C_002868"
     CDS             complement(22142..22393)
                     /gene="yecJ"
                     /locus_tag="ACHJ8C_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO5552693.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(22507..22593)
                     /gene="azuC"
                     /locus_tag="ACHJ8C_002869"
     CDS             complement(22507..22593)
                     /gene="azuC"
                     /locus_tag="ACHJ8C_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO5552694.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
BASE COUNT         5605 a         6095 c         5350 g         5590 t
ORIGIN      
        1 caacaaacgt tccagcggtg tatcgaggcc aataacatag acttcatctt tgccaggaaa
       61 ccactgctgg gtaatcgccc gtcccaacgc tacgcgcacg acggcggtta attcatgcgg
      121 atcgctttgg atgggcgcat gttccgccag cgtttcgaga atggtgcgca tatcgcgaat
      181 cggcactttt tcatcgagca gattttgcag cactttatgc agtgtggtaa gcgtgacgac
      241 gccaggaacg agatcttccg tcagctttgg catctcctgg gcgacgcgat ccagcagctg
      301 ttgcgcctcc tgacgaccaa acagctctgc ggcatgctgg ctaatgaggt ggttaagatg
      361 cgttgccacc accgtgctgg cctcaaccac ggtgtacccc tgaatctgcg cctgctcttt
      421 aagcgcactt tcaatccaga tagcattcag gccaaatgcc ggatcgacgg tcgcctcacc
      481 aggtaacgtc ccggcagcgg ttccagggtt aatcgccaac caacgccccg gataagcatc
      541 accactgcct atctccacgc ctttcatcaa aatgcgatag cgggcaggtt gcagatccat
      601 attgtcgcga atgtgtacca ctggcggcag aaaccccatc tcctgggcaa atttcttgcg
      661 gatactgcgt atacggccca acaactcacc atcctgctgg aaatcgacca tcgggatcag
      721 tcgataaccc acttccattc ccagagaatc ttccagttgt acatcgttcc acgtcgcttc
      781 gacaacggta ttattctctg ccatttttac cggttttgct tcggcaggcg ctttttgttc
      841 acgtccgcgg atccaccacg ccagccccag caatccggca gtgaacagca aaaataccag
      901 gttcggcatt ccaggcacca ggccgagtaa accgagcacg gcggcgctta ataacataac
      961 gctcggatta ctgaaaagct gattcaccat ctgctcgcca acatcctgat cggtgctgac
     1021 acgtgtaacg atgacgcccg cggcggtaga aatcaccagt gccgggattt gtgccaccag
     1081 accgtcgcca atggtcaata gcgtataact ttccgccgcg tgccccatgc tcatgccatg
     1141 ttgcagcaca ccgaccagca atccgccgac aacgttaatg accatgatga ggatcccggc
     1201 gatggcatcg ccgcgaacaa acttacttgc cccgtccatc gagccgtaaa aatcggcttc
     1261 ctgagtcact tcggagcggc gttttttcgc ttcatcttca ccaatcaatc cggcgttaag
     1321 gtcggcgtca atcgccatct gcttacccgg cataccatcg agaacaaagc gcgcgcccac
     1381 ttccgcaata cgcccggcac ctttggtgat aaccataaag ttgatgatca cgagaatgac
     1441 gaacaccacg ataccgatag cgaaattgcc accaacgagg aagtgaccga acgcttcgac
     1501 cacctttcca gccgccgccg cgccggtatg cccttccatc aaaatgatac gggttgaagc
     1561 cacgttaagt gccagacgca acagcgtggt aaacaacagg atggtcggaa acgcagcaaa
     1621 ctcaagcgtg cgctgggtaa acatcgccac cagcaacacc atgatcgaca aggcaatatt
     1681 gaaggtaaac agcaggtcga gtatgaatgc gggcagtggc agtaccatca tcgacaagat
     1741 caacaggatc aaaatcggtc cggcaaggat ctgccattgt gtcgctttca ggtttgcagg
     1801 caggcgcagc atcgcggcca gattactcat gggtcggttt ctcgttaata aaatccaggg
     1861 cttccggcac cggaagatga gtaggttgta cagggcgttg tccaccagcc agacgccagc
     1921 gtttcagttg ccagacccag gccagcactt ccgccaccgc ggcgtacagt tgacccggga
     1981 tttgttgacc gatctccgca tgtcgataca gcgcacgcgc cagcggcggc gcttcaagcg
     2041 tcgggacgtt attttcagcg ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt
     2101 tagcgaccac tttcggtgcg ctcattttgt tttcgtcata ctgcaacgct accgaatagt
     2161 gggtcgggtt attgacaatg acatccgctt tcggcacatc ggccatcatc cgacgccgtg
     2221 cggccgctcg ctgcatctga cggatccgcc ctttaacatg ggggtcgcct tcgctttgtt
     2281 tgaactcatc acgaatatcc tgccgtgaca ttcgcagctt tttcaggtgg ctgaagattt
     2341 ggaaaaagac gtcaaatccc accattggaa tgacaccaag caccaccagc agcgcgcata
     2401 gccctaccag atccatcgca ttacccatgg cggtaatcgg agactcggcc attaagcgca
     2461 tcatctgcgg ccagtgatgc cagagaaaaa accccgtcac gctgccaacc aggatggttt
     2521 tcaaaattgc cttaagcaac tccgcgccag tctgtgccga gaacatccgt ttaatgcccg
     2581 gtagcgggtt gagtttggaa aacttcggct gcaaggattt tccactaaat accagccccc
     2641 ccaacataac cggagaaata atcgccacca gcaccacgcc gctaatcagc ggtaacagcg
     2701 ccagcatggc ttctctgatc agcagaataa tctgcccgag gatcagattc gggtcattga
     2761 taatactgtg atcaaaatgc agccctgcgg agagcatgcc cgacaatgga cgggccagcg
     2821 ccacaccgcc aaaccagata acactaacgc ccaccagcaa aatcagcagt gaagtcagtt
     2881 cacgggaacg cgggatttgc ccctcttccc gcgctttttc tagtcggtga ggtgtggggg
     2941 cttctgtttt gtcgtcgctc tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg
     3001 taaatgatgc cagagcgcaa agtgttcaat ggtttgagta aggggcaaaa caggcgggat
     3061 ttagggcttt tgccacagca catcaggcat ggtgacatgt ttgtcgggta cggcgtaaac
     3121 gccttatcca accttgaaga tctgatgtga cgcggccacg tcacatcagg caatacaaat
     3181 caaaatccaa gactatccaa caaatcgtcc acctgatcct gactggctac cacaccggct
     3241 ttgctggtat cgacctgagg tccattaagc aaactctggt tttcacgttt tggacgcgac
     3301 tcctgttccg ggatgttttc caacagcacc atcagcaact gacgttcgat ctcctgaatg
     3361 acatccatca tccgcttaat gacctgtccg gtgagatcct gaaaatcctg cgccatcatg
     3421 atttccagca gttgcgcgtt agtaaagctg gtatgtgcgg ggacatctgc cagaaattgc
     3481 cgcgtgtctg ttaccagttc acgggcgtcg gcaaggtcaa tcggatcggc aaaccagtca
     3541 tcccaacgtt gggttaacgc ttttgctgat ttctccattt gatcctgatg cggttgtgac
     3601 gcctcaacac tgttcagcgc ccgctccgca gcctgggcgg tcatctgcac aacatagtac
     3661 aaacgatcgc gcgcatcggg gatagcttcc gcggcttcgg caatggcctg atccagcccc
     3721 agttcccgca aactgtcgcg cagcatacgc gtcaggctgc cgatgcgcgc aatgatatcg
     3781 ccagctgaat gctcgtcagc aggtttgatt gatggttgca tcatagtcgc atcctcacat
     3841 gcccagtttc tcaaagattt tgttgagttt ttcctccagc gtcgcggcgg taaatggttt
     3901 caccacatag ccactggccc ccgcttgcgc cgcagcaatg atgttctctt tcttcgcttc
     3961 tgcagtcacc attaacactg gcaatgccga catcgcgcca tccgcacgaa tcgttttcag
     4021 caactccagg ccatccatat tgggcatgtt ccagtcggag ataacaaatc cataaccgcc
     4081 tgcctgcaac ttattgagag cgtcgaggcc atcttccgct tcctcaacat tattgaatcc
     4141 cagctctttc agcaggttac gcactatgcg tcgcatggtg gaaaagtcat ccacaaccaa
     4201 aaatttaagt tctttatccg ccatttcaca ctcctgattt aaatacgtat cgcctgtccg
     4261 gcactaattt ttgccagcat ttgctgactt acctggctaa gatcgaccac ttcgcagaca
     4321 ccacccatat tgatggcctc gcgcggcatg ccgaacacca cgcaacttgc ttcgttttgc
     4381 gcaagggtcc atgcccccgc ctgacgcatc gccaacattc ccgccgcgcc gtcgttgccc
     4441 ataccggtta ggattacccc aaccgcatta cgccccgcct gtttggccac agaatggaac
     4501 aacacatcta ccgatggccg atggcgatta accgccgggc catcgtgaat tttgatttga
     4561 taatttgcgc cactacgcgc cagctccata tgccgatcgc ccggcgcgat ataggcatgt
     4621 cccggcaaga cgcgctctcc gtcttcggct tctttaaccc cgatctggca aagcttatta
     4681 agtctgtcgg caaaagagcg ggtgaaaccg ggcggcatat gctgggtaat taacagtgcc
     4741 ggactggaaa gcggcaacgg ttgcagtacg tgacgaattg cctcagttcc acccgttgaa
     4801 gcaccaatcg caatcagttt ttcagaactc aacaacggcc ctgccttcag cgttgtcggt
     4861 accgacaatg gcttatgtgc tgcaaggctc gcctttgctg ccgtacgcac cttttcagca
     4921 atcatttcgc tatatgccag cattccttcg cgaataccca gttgcggttt ggtgacaaaa
     4981 tctatcgccc ccagctccag cgcgcgcagc gtaacttctg accctttgcc ggtcagggaa
     5041 gaaaccatca caacgggcat tggacgcaaa cgcattaatt tttcgaggaa atccagtccg
     5101 tccatccgcg gcatttcaac atccagcgtc aatacatcgg gattgaattt cttaatcaaa
     5161 tcacgcgcaa ccagcggatc aggggcggtc gccaccattt ccatgtcgct atggctgttg
     5221 atgatttctg tcatgatctg gcgcatcagt gccgaatcat cgacagataa caccctgatt
     5281 ttgctcatcg ttaatcctta cttagcgcat acaccgtctg accacgcagc gtgaagcggc
     5341 gctcaaggtg gctaaagttt tcagagtgac ccgcaaacag caatccgtcg ggtttaagga
     5401 gcgaaacaaa gcggcgcaaa atctcctgct gggtagtttg atcgaagtag atcatgacgt
     5461 tacgacagaa gatcgcatca aacggccccg gcacggtgta ctgtttcgcc agcaaattca
     5521 gcggggcaaa atcaacatag ttcgccagct cctgacgcac gcgtaccagc ccttcatgcg
     5581 gtcccgtccc tcgcatgaaa taccgttgca gttgctgcgg cgtcaggttt ttcaactctt
     5641 catggcgata aataccgctt ctggcttttt ccagcacttc ggtgtcgata tcgctggcaa
     5701 acactttcca gcgtccgggc gcggtgccca atgtatcagc cagcgtcatc gctatgctgt
     5761 acggctcttc gccggttgaa gccgccgcgc tccatacgcg atactcgcca gaacggcgac
     5821 gtgcgtgatc cgcgagcaga ggaaaatggt gtgcctcacg gaaaaatgcc gtcagattcg
     5881 tggtcagcga attgataaac gcctgccact caccgctgtg ctgattagat tccagcaagt
     5941 tcaggtaatg accgaaatcc gccagtccca gcgcacgcaa acgacgaacc agtcggttgt
     6001 aaaccatgtc gcgtttatgg tcagccagaa cgatcccggc tcgttgatag atcaattgac
     6061 ttatccgccg aaaatgcgcg tcggaaagcg ccaggcgctc ggtcatctgt aacaataaag
     6121 acgtttgccc acagggcaga gatgaagtca tagcgccttc tcaatcactt caggatacca
     6181 ctggcgcaat ttgtaactgc gccgactcat gtcgtgctac ttcatgttct tcaagggtaa
     6241 ataccgccac acgcgacgaa agatggtcgg cctgattcag tcgttgcaaa tcgtcgcgaa
     6301 atgcccaaaa gacatgccgt tactgccaat ctgcaagatc ccgcagagaa tcaaaattaa
     6361 aaacagcgtg gtcgaaattc gaatacgatt aaacatcaac gctccccatc aggcggcaat
     6421 gaccgcttta gtaaatactc gtcaaaatgt ttcccagttt ggatcttgtt cagcaattcg
     6481 cagtcgtggc tgtgcaggtg gttgctcact ggcaggacgg gatggtgttt gcggtttatt
     6541 ggtgagtggg ctggctgcca gacggaacgc ggaaaccgct tgcgttaaac gactcgcctg
     6601 ttcttccagc gcagctgcgg cggcagctga ttcctgcacc agcgatgcgt tctgttgcgt
     6661 gacgcgatcc atttccgaaa ccgccaatgc gacttgatcg atgccacgac tctgttcatc
     6721 cgatgctgat gcaatctcgc ccataatgtc agtcacgcga gtgacagcat tgacgatatt
     6781 gttcattgtt tccccagcgc tttcgaccag caccgaaccg gtatcaacgc gcgagacgga
     6841 gtcttcaatg agggctttga tctcttttgc cgcctgtgca ctgcgactgg caagattacg
     6901 cacttcaccc gccaccacgg caaaaccacg gccctgttca cccgctcgcg cggcttcaac
     6961 agcggcattc agcgcgagga tattagtctg gaaggcaata ccatcaataa cgctgataat
     7021 gtcggcaatt ttcttcgaac tatcggcgat ctcatgcatc gttttcacta cgccatccac
     7081 cactttgccg ccgtgctggg cggtgtcgga ggcactttgc gccagttgcg aggcctggcg
     7141 ggcgttatcg gcgttttgct tcactgtcgc ggtgagctgc tccatgctgg cggcagtttc
     7201 ttccagcgcg gatgcctgct gttcagtacg ggaggaaaga tcggtgttgc ccgccgcaat
     7261 ttcacgggta ccggcataga tggcatctga accttcgcgc acatgagtga cggtgtcagt
     7321 caaagagcgt tgcatatgtg aaacgctctg cgccaggtcg cccatttcac tgcgcccgtc
     7381 aatggtcagg gtattcgcca ggttaccacc ggcaatttcg cgaatatgcg caataatttt
     7441 tgccagcggt gtaagcaaca tacggcgaat accgtaccac gccaccagca gaatcaatac
     7501 caccaccagc gcgataaccg ccagttgcca ctgggcaaat cggtaatcat ctgcgttgtc
     7561 agtgacgata tcgcgataca gtttttcact gctgagggcg tactgagcaa acgcttcgcc
     7621 cattgcattt tgcattccct gggttggctg agcgaaataa gctccagtat tgccataatc
     7681 aagataatca atcagttcag ttaacgctgt gtgatagttt ttatattttt catcaatatt
     7741 acgactggta gcgaccattt caggtaacgg tgccatgctt ttgaattttt tataatgagt
     7801 cgcggcctgg gccaatgttt tcctggcgct atcgagcaat tcaactttgg cattactttg
     7861 ctgattggat gaatccatca tcatccgcac cgctgaacga ctcaggttaa tgcgcgtttg
     7921 cagcattaaa tcccatgttg acgtcagctc gccctgctgt tcccgtaatt gattggaaac
     7981 cacaaagctc ttctggctat ggtgaaggga agaaaaaaac agactgccgg aaataagctg
     8041 taacagtgcg aataccccca gcaccattac caacagcgtg actacgcgga tacggttaat
     8101 cataaggcac cttcctgaaa acaagttgat ctcgttatcg gcaaggaggg gggaaacttt
     8161 attgctgatg ccacccgccg cgcaattgaa ataaaaaacc cgatgcgcag atcatcgggt
     8221 tcatttcaat tgaggaaatc gggagaatta cgccacttct gacgccgcgc tatctaacag
     8281 cgccatctct tcgctgttca gcagcttttc gatgttcacc agaatcaaca tgcggtcgcc
     8341 cagtgcgccc agtcctgtaa gatattcggt tgaaagcgtc acggcaaatt ccggtgccgg
     8401 acgaatttgc tccgccgtca atgaaagcac gtctgagacg ccgtcaacca cgatgccgac
     8461 cacccgctgt ccgagattca ggacgataac taccgtgttg tcgttatagt ccacatccac
     8521 ctggctgaac ttaattcgta agtcaacaat cggcacaata acgccgcgca gattcgtgac
     8581 gcctttgata aacgctggcg tgttcgcaat ccgtgtcacc tgatcgtagc cacggatctc
     8641 ctgcactttc agaatatcaa taccgtactc ttcatcacca agggtaaata ccagaaattc
     8701 ctggcctgac ggctcgctgg ccagctttgt tacattcgtc ataccggtca tattgttacc
     8761 tttttactca ttcaggcggc ggtgttcgcc atacgttgtt cgcggtttat cgcctgcaag
     8821 gcggagacat caacaatcag tgccacgctg ccgtcgccaa gaatggtcgc agcagaaatg
     8881 ccggggactt tgcgatagtt actttcaagg tttttaacca caacctggtg ttgaccaatt
     8941 aattgatcca ccagcaaggc gtagcggcga ccgccacttt gtaagatcac cacaattccc
     9001 tgggtggctt cggttttcgc gcccgcgacg ttgaacactt tccacagttc gacgatgggc
     9061 agatattcac cccgcacttc cagcacccgc tcgccgccag ccagtggatg cagatcggct
     9121 tcacggggtt gcagtgattc catgacagca ttcagcggca gaatgaaaac ttcatccgca
     9181 acgcgtacgg acatgccgtc aagaattgcc agcgtcagcg gcagtaaaat gcggatcgta
     9241 gtgccagtac cctgcttcga ctggatctcg acatgaccgc ccatctcctg gatattacgt
     9301 ttaacgacgt ccatgccgac gccacgcccg gagacgtcgg tgacctgctc cgccgtggag
     9361 aagccaggtg caaatatcag cattgcaact tcgtcgtcgc tcatgttttc gctgacggtc
     9421 agaccttgcg aggccgcttt tgccagaatt cgttcacggt ttagccccgc cccatcgtcg
     9481 gtcacttcaa tgcaaatgtt gccgcgctga tgttcggcag acagaattaa atttccgacg
     9541 ctgtttttac ctgcagcgag ccgtttttct ggcagttcaa taccgtgatc gaggctattg
     9601 cgtaccaggt gggtcagcgg gtcgataatg cgttcaatca ggctcttgtc gagttcagta
     9661 gaactgccca ccagcgtcag ttctacctgc ttgccgagtt ttcccgccag atcacgcacc
     9721 agccggggat agcgactaaa gacatattcc atcggcatca tgcgaatcga catcactgat
     9781 tcctgcaaat cacgagcgtt acgttgtaac tgccccatgc tggtaatcaa atcaccatga
     9841 ttaaccgggt ccagttcact ggagcgctga gcaagcatgg actgggtgat gaccagctcg
     9901 ccgacgaggt taattaatag atcaaccttt tctaccgcta cacggatgct ggtggattca
     9961 ctgctgcgcg tcgttttttc ccgctccacg cgaccggttg gcgcttgttc ggctgccagt
    10021 ttaagcactg gtggggtgga tatttttggc gagacgtcta ctgtttcaaa ggtaatctga
    10081 tcggcttcaa tcacaaaaca gagtaccgct gtgatgtcat cttcggcgat gtcgcccggt
    10141 aatattgccg agagagaatc cgcccctttc accacgtcag ttaacgttgt cagatgcccc
    10201 aactcttctt ccagcaggtc gacttcgcgg gccttcaggc gcgaaaggat aattcgtcgc
    10261 ggtgactgac tacgactctg ctcatcttgc ggttcacttt tggcaaccac acttaatcgg
    10321 gtcactgcag atggcgtttc gcctttcgct tctaatgcca gttgacgcaa ggcctggcag
    10381 atataatcga agctggcggc atccggctct tgcgactgtt tataagcgtc gagctgttct
    10441 tgcatgatgt ccttcgtttc caaaaacaga ttgataatgt cggtgttgag ttgcatctca
    10501 cctcgtctgg cttcatcgag caggttttcc atcagatgcg tggtttcctg caaaacgctg
    10561 aagccaaagg ttcctgcccc tcctttgatc gagtgggcag cccgaaagat ggcattcaac
    10621 tgttcggcat ctggcgcttc cggctgcaaa accagcagat gctgctccat atcagccaac
    10681 agttcgtccg cttcatcaaa aaatgtctga taaaaatcgc ttatatccat gctcacgctg
    10741 tcacctcggt tcggctgatg gcattgtggg aacactgacc tgtggtgcaa cctcagtttt
    10801 ttccagggcg cttactggct cattctggct ttcggcgttt tcatgcaaaa tggcctgttc
    10861 ggcgtgtttg ttcagtacca gcaggctgat gcgacggttg acggcatcat caggtccgcg
    10921 atcgcttaag cgcatcgttg ccgccatgcc gacgacacgt aacactttgc cgccattcaa
    10981 cccaccaacc atcagttcgc ggcgggatgc attggcacga tcggcagaaa gctcccagtt
    11041 gctgtaacct ttctcaccac tggcgtaggg aaaatcatcg gtatgacccg agaggctaat
    11101 tcggttggga ataccgttca gtacgggcgc gatggcgcgc agaatgtcgc gcatataggg
    11161 ttcgacgtcg gcactgccgg ttctgaacat cgggcgattc tggctatcga tgatctgaat
    11221 acgtagacct tcctggacca gatcgatttt gagatgtgga cgtaacgccc gcagtttcgg
    11281 atcggactca atcaactggt cgagatcgcc acgcaatttc cgcaatcgac tttgctccat
    11341 gcgttttttc agctcttcga tgttcggctg cttattcact tccccctggc tttgggtgta
    11401 atcatcaccc ccaccgggaa ttgggctttc actattagaa atgcgatcgc cgcccgtaac
    11461 cgcagtcgcc agtggagtcc ggaagtactc cgcaatctga atcagctctt ttggactgga
    11521 gatagagatc agccacatca ccagaaaaaa ggccatcatt gcggtcataa agtcggcata
    11581 agcaatcttc cacgatccat gtgctgcccc gtggcttttg gctttgcgtc gtttgacgac
    11641 aataatcgga tgcgcttgat tcttcatgct tcctcggttg tcgtctgttg ttgcggattt
    11701 ttcaccgcac gcacatgctc ttccagctca ataaacgacg gacgttcgct ggaatagagc
    11761 gttttgcgac caaactcaac ggcgataggc ggtgcgtaac cgttcagatt tgaaagcaga
    11821 gtgactttga cgcactgcat cattttgctg gtttcggcgc ttttctgacg taaaacactc
    11881 gctaatgggg aaataaatcc gtaagccaat aaaataccga ggaaagtccc caccatcgca
    11941 tgagcgataa gcgcccccag ctcggcggca ggacgatcgg ctgaacctaa cgcgtgaacg
    12001 acccccatta cagccgcaac aataccaaac gccggaagtg agtccccgac cagcgccaga
    12061 ctgttcgccg ggacttctgc ctcgctttcg tgcgtctcaa tctcttcatc catcagagct
    12121 tcgatttcga aggtgttcat gtgaccgctg ataatcaggc gcagataatc gacgataaat
    12181 tcaagcatga cgctatccgc aaggatgcgt gggtagctgg cgaagatctc gctctcacgg
    12241 ggattttcaa tatcgcgttc cagcgaaaac atccccatct gccgcgattt cgccatcagc
    12301 cgataaagca gagccagcag atccatatac attgctttgg tgtatttgga gcgacgaaac
    12361 agcaacggca gcgccttcag cgtgccttta atcgctttgc cattattgcc gacgataaac
    12421 gacccaatcc ctgcaccggc aataatcacc agttcagcgg gttgatagag tgctccaagg
    12481 cttccaccgg tcatcaaata accgccgaaa actgtaccga gaacaaccag gtaacctaat
    12541 aagataagca cgacatcatc cttccactgt tgaccatgac aggatgttca gtcgtcaggc
    12601 gttaacacgc gataggggca aaaaaaagca gcggtacgtc gttaccgctg ctggaatgtt
    12661 tcgccacacc gtatcagtta aacagcctgt actctctgtt catccagcag ttgtgggata
    12721 atatcggcag gattctggga aagtttacgt ctttttactg cccgggatgg cggctgacat
    12781 aagctgcagg caaagctgcc aacaggctgg tgagcatggg taataaaatt gccgccgcag
    12841 cagttgcagc tggaaagttg cagtaatcca ctttcaacaa accgcaccaa tgtccaggca
    12901 cgggttaatg ccagcagcgg tccttcttct gcttgcgggc actgttcaag gtataaacgg
    12961 taggctttga tcaccgcatc gacgccatta cacaagccag ttttcagtaa aaactgccat
    13021 gcattacaga acatcgaagc atgaacattt tgttcccagg tcataaacca gtcggttgaa
    13081 aatggcagca tgcctttcgg cggtgggctt ccgcgcagtt ctttatagag ttttatcaga
    13141 cgtccgcgac ttaactgtgt ttcgctttcc agcatctgca aacgagcgcc cagggtgatc
    13201 aattccattg ccagctgaat atcccgcgct tcctgaacaa tgcttttttc actcatgatc
    13261 aggccctttt cttgcgcagc gcttcttcag gctgattaac atcattcagc aagcgtgttg
    13321 agagcatgat gccggtatga atttgctgga gatcgtcaac gcgggaatct tgcgtcaact
    13381 gagtaatcgt ctggtggctg tcaaaacgga agtgacaaac cagttgattg gtttctgcca
    13441 gcttaaccat ttgcggaaga gtcagtgccg ctaacgttgt cgccatttct tcatttatgc
    13501 cgagacgaaa catagcggac gctttgtcct gaacaatcag acgctgtgca agtagtaaat
    13561 atgacaagtt gatgtcataa atgtgtttca gcaactcgga ggtatgcatt attcccaccc
    13621 agaataacca actttatttt tatgcggtct caccgcaccc cgtgatgtcg ccgggaagcc
    13681 ccggtaaaaa ataattagca ttagaatagt tgcgataagc tgcaataagc agacccacct
    13741 ttttggttta ataagtcctt acaaatagaa gggggtcttt acacttatct aagatttttc
    13801 ctaaatcgac gcaactgtac tcgtcactac acgcacatac aacggagggg ggctgcgatt
    13861 ttcaataatg tgtgatgcag atcacacaaa acgctcaatt acttaacata aatgtataag
    13921 tcatactttt gttttgggtg tatttcaatc tgttaaaaaa tttttcgcta cactggcaag
    13981 caaaaatgaa acaggaataa tcgaaatggg atgttgcgca cagttaaaat aactcaccgt
    14041 aaataatcat ctgctataaa taatcgcttt aatgcaatac cagataagcc atttttaaac
    14101 aaacacttac cgcacaacaa actaattaac tacaaagaaa ggcaacaatc aacctaatgt
    14161 taataaaatg ttttacatat attaaccact tgttcaatat gggaaatcag tacattattt
    14221 tcttataaat acagttgaaa tcatatgata accaagagca attgattaat tgtatttact
    14281 aatgaccaga ctaatattta cctgtttaac tgagttggga ttgcgtcgtt tctccattag
    14341 gagtaaagct ttaatgtcac ctgaagttca cagaataagg aacaggttat gagctatagc
    14401 aatattcttg tcgccgttgc ggttacaccg gaaagtcagc aactgctggc aaaagcggta
    14461 tctatcgcca ggccagtaaa gggacacatc agtttaatta ctctcgcttc cgacccggaa
    14521 atgtacaatc aattagctgc gccgatgctt gaagatttac gcaatgtgat gcaggaagaa
    14581 acgcaaagct ttcttgataa gttaattcag gatgcgaggt atcccgttga caagacgttt
    14641 attgcctacg gtgagttaag cgaacatatt ctggaagttt gtcgcaaaca tcatttcgat
    14701 ttggtgattt gcggtaatca caatcacagt ttcttttcgc gagcatcctg ctcggcgaaa
    14761 agagtgatta cctcaagtga ggtcgatgtg ctgttagttc cacttacggg agattaatcg
    14821 cacctacgca agcttaggaa aggtagcaac tttatcgcgc tgctggcttt ccgcgcttcg
    14881 cggaactatc tgctttaggt cgctaatgaa gcactcctgc cagtggttaa tatcgttttt
    14941 cacgataacg tccagcattt ctgcatgacg ggaaatacgt tccgccagcg acatagtcaa
    15001 tgcacgatcc agcgcagctg caacttcgtc acgatcgtag ggattaacaa ttaacgccga
    15061 cgttaactcg tttgccgctc ccgcaaattg cgaaagaaca agaacgcccg ggttggctgg
    15121 gtcctgagcg gcaacatact cttttgccac caggttcatt ccgtcacgca gtggcgtcac
    15181 taagcccacg tcagagtagc ggaatatttt catcagtaat ttacggtcaa aatgctgatt
    15241 caaataataa agcggcgtcc agcctaattg cccatattta ccattaattc gtccggcttc
    15301 attttcgagc tgatgacgaa tatcctgata ggcttgcaca tcaccacgag acgttggcgc
    15361 gatctgggta taacgaattt taccgtgatg ttgcggatat ttttccagta acgcttcata
    15421 ggcgagaaaa cgctccggca aacctttgga ataatccagt cgttcgacag aaaagatatt
    15481 ttgtacgttt ttcagttctg ctttgagttg cgccagtttt ggcggcagtg gaccggcagc
    15541 ctgtttcgct atttctttcg gctcaatgcc gatcgggtaa acttccgttc gaaacgcttt
    15601 gccgcaggcc gtatggcttt ttgcgctacg tgtcgtgacg cgggtcaggt tagaaaggca
    15661 atccaggaac gccagacgat cgttctccgt ctggaaaccc agcaaatcat aatcaccaag
    15721 ctgttcaagc aaggtgtcat atgtcggcag cgcgttgaag atttccggtg tcgggaaagg
    15781 aatatgcaga aagaaaccaa tgcgattatt cacgccccgt ttgcgtaatt catgcgcaaa
    15841 gggcaacagg tgataatcgt ggatccagat aatgtcatcg tcttgcaaca gcggcagtaa
    15901 tttatctgcc agcaacgcat ttacgcgtag atagccatcc caggcaggac gctgaaattg
    15961 caccagatcg agccgataat gaaaagcggg ccagagaacg gcattggaga atttgttgta
    16021 gtattcgtca aggtcctgtt cgctgaggtt aaaagaggcc cacgtaatgt tacccttttt
    16081 cacctttttt agcggctgat cctcattccc tgtttcaccg ctccagccaa accacagtcc
    16141 acctgcggct ttcagtgccc ccagtatgcc aacggcaagg ccaccggcac tggcggcgtg
    16201 ctcgtctggt ggtgcaatcc ggttagatac tacgactaaa cgactcatag tcatcactcc
    16261 tgttattttc tcttttttgt tgtaatgcgg tggttatcat ttcaagccag ctccagacat
    16321 ccggcacgcc cgccagtcgc catgatgcct gagttgcacc tgtgccaatt tttactgaca
    16381 ttccgcccag ccggttaacg actgcgaagc cagattcatc ggtcagatca tcgcccagaa
    16441 atacgggcgt tcgcccgata aagggagctt cctgcataaa ggcagcaatc gcctcacctt
    16501 tactggtacc tttcggtttg atctcgacaa cacactttcc ctgctgtaac gccatttgtg
    16561 gccagatctg agtaatacgt tgcgctaatg tcattaatgc gtcttcatgc tgcggagcct
    16621 ggcgataatg cagcgcaaaa gccatccctt tcgcctccag ctccgcgccg ggatactggg
    16681 cgatgaccgt atgcagttgc acgctaatat cacgcgcaat cgcatccggc agatgaacga
    16741 tatgcgtttt accattgatg tcacggcgct ccgccccatg cacgcccgct aacgggaagc
    16801 gataaggttt tgccagtgcg tcaagctcca ccattgagcg ccctgaaatc aatgccaatg
    16861 caccatcact tgcggttgcc agtagctgaa gtccttgcag aatagtgtca ggcacgacga
    16921 cctgatcggg atgcggtttg atttccgcca gcgttccatc gagatcaaaa aaccaggcat
    16981 atttcgcgga tagttcaggg gtttcggtta acggttctgt cacccggttc tcctccttct
    17041 cttttcttat tgtgttgcgc agaacgcgct ttacagacat gtaagtatag acagtgtgac
    17101 gggggtcgcc atttgcaacg aagaacagcc aaccgttgtg ggggctggct gtggtgggaa
    17161 aaaatgctaa attgttgtag aaaaaagcat cagacagtgc gtttcgcttt ttgcttgtaa
    17221 cggtcgaaaa tcaccgctgc cagcaggatt aagccgcgaa ctacgtactg tgcgaaagga
    17281 gagatattaa gcaggttcat ggcgttttcc acggtgccta aaattaagat acccgccacc
    17341 acatatgaga tttttccgat gccgcctttc agagaaacgc cacctaaaac gcaggcggag
    17401 atcacaatca gctcataacc aatcgacgtc attggctgcc cactggtcat acgtgaagcc
    17461 agaataattc cggctattgc tgataccagg ccagagagaa caaaaataat aattttggtg
    17521 cgaacaaccg gtacacccgc cagacgcgca gcctcttcgt tcccgccaat cgccagggta
    17581 ttacgaccaa aggtggtttt attcagcagc aagccaaaga tgatcagaca cgcgacggtg
    17641 agccagattg gcgcaggcag accgaaccag ttggcgtaac caagggcaaa gaagctttca
    17701 tcttcgatac cgaccgcttt accgtctgaa atgatgtacg ccagacctcg aacaatctgc
    17761 atcgttgcca gtgtcgtgat cagggcattt attttgagtt tggcgataac aaagccattg
    17821 accagaccac agagaatgcc cagcagcaac cccgctgcca cgccaatcca caggctttca
    17881 gtcagattaa taaccaccgc cgtggtgaca cccgcacagg caattacgga ggcgacagaa
    17941 aggtcaaagt caccggaagc aaggcagaac aacatgccac aagccaccat ccccgacatg
    18001 gaaattgcca ggcccaaccc tttcatatta atgaaggtgg caaaatttgg gacaaaaatg
    18061 gcacaggcga taaaaagcac cgcaaacacc accagcatgc cgtactgatc ccagatacgc
    18121 ccgaagctga atgacgactt aggtgcgcca gaacccgatg tagaaacaga agacatcata
    18181 ctctccttac tcaggcaact gcctggctga ctttaggcat cgcaaggctc agtgcctgac
    18241 gctcatctgc ctgctcgtgt aacaattcac cggcgatttc gccttcccgc atcaccacaa
    18301 tccggtcggc aacgccgagg acttcaggta agtcgctgga ggcaaacagc accgccacac
    18361 cctgcgccgc cagcgcataa atcacgttgt aaatttcatg cttagcacca acatcaatgc
    18421 cacgcgtagg ttcatccagc aaaatgacct tcatctcttc cgataaccag cggcccagaa
    18481 tggctttttg ctgatttcca cctgagagat tcataatcag ttgctcagcg cccggcgttt
    18541 tgatgttgag cgaacgaatg tggtgatcgg cattgttttc ttcccaaccg ttgttgatta
    18601 cacaaccgcc gagcacatgc ttgcgtctgg cactgatgtt gatattgtcg cgaacggagt
    18661 gcacaggaat aatgccttcc gctttgcgat cttccgggca gagcatcatg cctgcggtaa
    18721 tagcgtggct cggtttacga atatcgatcg gctgttggtc gatataaacc tgaccggcgg
    18781 tgatttgcgt accgccaaac atgcctttca ttaattcgct acgccctgcc ccgaccagac
    18841 caaacagacc gacaatttct ccactgcgaa ccgccagact tattggcgta cgcacgcctg
    18901 gtgctttcac agcatcaaga cgcagccgct cctcgccata actacgcggt tgccagccgt
    18961 agatatcgcc aatgtcgcgc ccgaccatcg cctgcaccag tgcgtcgtgg tcaacctgct
    19021 gcatatcggt aaaggttttg acataacgcc catctttaaa gacggtgatg gcatcgctga
    19081 gggcaaatat ttcttccata cggtgagaaa cgtataagat cacccgcccc tcttttcgca
    19141 gttcacgaat aacgcggaaa agattgtcga tttcgcgggc agagagggag ctggttggct
    19201 catcaaaggc gataattttg gcgttacgcg ccagcgcttt ggcgatttca accatctgcc
    19261 actgaccaat ggagagatat ttcagcggcg tgtccgggtc aatatccata ccaagatgtt
    19321 taatttgtaa acctgcctca taattcagca atgaacgatt cacaatgccg cctttatgcg
    19381 gcagctggcc gagatagatg ttttccgcga cggtcatttc cggcacgaga tgcagttcct
    19441 gataaataat cgccacaccc gcgttaagtg ctgcggtcgt gtcggaaaag gacatttcct
    19501 gcccattaat cactacagaa cccgtggttg gcgcatagtt tccgctgagg atttttaaga
    19561 gagttgattt tcctgcgcca ttttcaccca tcaacgcatg aacctgaccg gcatagcagt
    19621 caaaactaat atccgtcagc gccttaacgc cgggaaacgt tttaccgatg ccgcgaaatg
    19681 agagatacgg ggtagactgt tgcataacgt ctccgtgaat cactggtcgt tcacaccccc
    19741 tctgcatcat gcagagggga atttttccgg caaattactt accgcctaaa ccttttttct
    19801 ccagttcttc tttaaagttg tcacgcgtga tcagcaccac gtcggtaact tcggtgaatt
    19861 tggtcggttc aacacctttt gcaacccagt tgtaaagcat ttcgctggac ttataaccat
    19921 gtacgtccgg gcttggcagc agggaaccgt agaagccggt tgcctgtgcc ttagacagtt
    19981 cactcaccgc atccacaccg ttaataccga taccgatgat atcgctcgct ttaaagccct
    20041 gaccttccgt cgcgcgtacg ccgcccagca cggtgctgtc gttcataccg acaatcagcc
    20101 aatgtttaac ttccggatgt tgaaccagca ttgagttggc agcgtcaaat gcccccggga
    20161 tgtcgttaga tttggtaggt acctgataaa tttgtttttc cgggaatcct gcagctttca
    20221 gcgcatccat agaccccgta gtacggcgac gagccgtatc cagttcgttg gaggtaatcg
    20281 ccatcaccgc actttctttg acatcccagc cacgtttctg catctcttta tacagttcct
    20341 ggccctgacg ttcgccaatt ttggtcgctg ccatcatcac cagcggaacg gtatccattg
    20401 gcttaccttt ggcgttaaca aactggtcat ccacggcaat gactttcata tcgtagccac
    20461 gcgctttcgc cacgatggct gagccgagtt tcgggtccgg agtacaaata acgaaacctt
    20521 tcgcgccact ggcggccagg ctgtcgatag cgttcaatgt tttttcgcca tccggcacgg
    20581 caatcttaat aacttcaaac cctaaatcct tcccggcttt atcggcaaac ttccattcgg
    20641 tctggaacca cggctcttcc ggttgcttca ccaggaaacc gagcttcagg ttctccgcca
    20701 tagcggattg tgacataacg gctgccagac caatggctgc cagggcttta gtaaatttgt
    20761 gcatggttct ctccagcttt agtgtcgttt tgtgtagggc aaaaacgaat gacattcgtt
    20821 aaattaatcg gaaaacaaag cattaccttt taactaaaag ataagtgact gtgttgacat
    20881 agttttagcg agaaattaat tctccatagg agagcaatat cacatcgcag aattacagtg
    20941 agaacgtgca taaatttagc gggaaaagac ataaggaaaa gcgaatttgt cagacaaatt
    21001 atcgagtgca cagcagaata atccataaga ttagcctgga agtccttgtt gtctttggta
    21061 cccatgcggg atgtcttctt tttaaccagt caataggcct cattacctgg cgttgagttt
    21121 ttgcaatggt gtaataaccg caacttaaag atgtggaaaa tgcgcgtcat tcatttcgcc
    21181 attaattatc actgtactca ttaattaaca gaacacgtat aatgagagcc atatcgcaaa
    21241 aatgaaaaaa cgttttatat aatcattgct tcatcatgaa ttcaaattca tcgattaata
    21301 tcaacaagat acaaaaagca ccatcattaa aattcattac agttacattg atttcatcaa
    21361 tgaaatgtaa aaatatataa acttgatgat ttaagcattt tcttataccc gttcagacgt
    21421 tattcttatt tcagctcatc gtcagaattg actccacgat cacatttcgg accggcagaa
    21481 aggaattatt ctgcaaacag taattatggt gttttgattt atcttgcacc tctccacttc
    21541 tggatataag gatattaggt atggcaaccg ctggaatgct tctcaaactc aactctcaaa
    21601 tgaaccgcga gttttacgca tccaatctct accttcacct gagtaactgg tgttctgaac
    21661 agagtctgaa cggcaccgcc actttccttc gcgcccaggc acagagtaat gtgacccaaa
    21721 tgatgcgcat gtttaacttt atgaagagtg tcggcgctac ccccatcgtt aaagccattg
    21781 atgttcccgg tgaaaaactg aactctctgg aagaactgtt ccaaaaaacg atggaagaat
    21841 acgagcaacg ttcaagcacg ctggcgcagt tagccgatga agcgaaagaa ctgaatgatg
    21901 attcaaccgt caatttcctg cgcgatctgg aaaaagaaca gcagcatgat ggcctgttac
    21961 tgcaaaccat tcttgatgaa gtgcgcagtg cgaaacttgc tggtatgtgt cctgtgcaga
    22021 ccgaccaaca tgttctgaat gtcgtgtcgc atcagctgca ttgatcatac atcggcgcca
    22081 atgcattgcg ccgatgaagt ttttgagaaa ccgctgcctc attttttgaa gcagcggttt
    22141 tttaatggga ttcaccctgt ggggtaaact tgagttcaat caacgcgatg gctttttgga
    22201 ttgcccgcat ggtgaccggg tctgcggcgg cgggatggtt agtaaagtcg atattcttca
    22261 gctggctgga cattttttcg cgaacttcaa cgggcgcgat cacatcgaga acatccagaa
    22321 tttgtttgat aaccaactgg caagcaacca catcagaaac cagttcctga tcggcattca
    22381 gcggctggga catcgtaaac tcctgatagc attttgaaag ccgttatagt agcggcttca
    22441 catcttcagc gatagtcaca tccaccgtca tcagaacaca aaaaacctgc cggagcaggt
    22501 tttttgttat cggaacatat tgcctggcgg tacgtctttg aacgtcttgc aatagttatt
    22561 gaacatactt ttcaggatct tgcgcagttt catcgcggta ctccgaccat ttgttataca
    22621 ggtgttattg tctttacgtt
//
LOCUS       DAZFJL010000078        22624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFJL010000078 DAZFJL010000000
VERSION     DAZFJL010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.078"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..436)
                     /locus_tag="ACHJ8C_002870"
     CDS             complement(<1..436)
                     /locus_tag="ACHJ8C_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO5552695.1"
                     /translation="MLAKDKTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSIFRGFSTQIQEHLAMGVLLS"
     gene            complement(446..997)
                     /gene="ybcL"
                     /locus_tag="ACHJ8C_002871"
     CDS             complement(446..997)
                     /gene="ybcL"
                     /locus_tag="ACHJ8C_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFO5552696.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(1166..1498)
                     /gene="emrE"
                     /locus_tag="ACHJ8C_002872"
     CDS             complement(1166..1498)
                     /gene="emrE"
                     /locus_tag="ACHJ8C_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFO5552697.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWFFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(1843..2157)
                     /gene="fliE"
                     /locus_tag="ACHJ8C_002873"
     CDS             complement(1843..2157)
                     /gene="fliE"
                     /locus_tag="ACHJ8C_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO5552698.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2372..4030
                     /gene="fliF"
                     /locus_tag="ACHJ8C_002874"
     CDS             2372..4030
                     /gene="fliF"
                     /locus_tag="ACHJ8C_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO5552699.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPGGK
                     PLPLSNEQMKQIEALTREAMGFSEKRGDSLNVVNSPFNSSDESGGALPFWQQQVFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAVKTVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            4023..5018
                     /gene="fliG"
                     /locus_tag="ACHJ8C_002875"
     CDS             4023..5018
                     /gene="fliG"
                     /locus_tag="ACHJ8C_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO5552700.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5011..5697
                     /gene="fliH"
                     /locus_tag="ACHJ8C_002876"
     CDS             5011..5697
                     /gene="fliH"
                     /locus_tag="ACHJ8C_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO5552701.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMAESEETIIEEVEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARIQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5697..7070
                     /gene="fliI"
                     /locus_tag="ACHJ8C_002877"
     CDS             5697..7070
                     /gene="fliI"
                     /locus_tag="ACHJ8C_002877"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO5552702.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7089..7532
                     /gene="fliJ"
                     /locus_tag="ACHJ8C_002878"
     CDS             7089..7532
                     /gene="fliJ"
                     /locus_tag="ACHJ8C_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO5552703.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7529..8656
                     /gene="fliK"
                     /locus_tag="ACHJ8C_002879"
     CDS             7529..8656
                     /gene="fliK"
                     /locus_tag="ACHJ8C_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO5552704.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDIVSDAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TFFTKLTSAQLTTAQPDDAPGTPAQPLSPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8761..9225
                     /gene="fliL"
                     /locus_tag="ACHJ8C_002880"
     CDS             8761..9225
                     /gene="fliL"
                     /locus_tag="ACHJ8C_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO5552705.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            9230..10234
                     /gene="fliM"
                     /locus_tag="ACHJ8C_002881"
     CDS             9230..10234
                     /gene="fliM"
                     /locus_tag="ACHJ8C_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO5552706.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10231..10644
                     /gene="fliN"
                     /locus_tag="ACHJ8C_002882"
     CDS             10231..10644
                     /gene="fliN"
                     /locus_tag="ACHJ8C_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO5552707.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSGKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10647..11012
                     /gene="fliO"
                     /locus_tag="ACHJ8C_002883"
     CDS             10647..11012
                     /gene="fliO"
                     /locus_tag="ACHJ8C_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO5552708.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVMVDVEDARLVLGVTAGQINLLHKLPPSAPTDEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            11012..11749
                     /gene="fliP"
                     /locus_tag="ACHJ8C_002884"
     CDS             11012..11749
                     /gene="fliP"
                     /locus_tag="ACHJ8C_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO5552709.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11759..12028
                     /gene="fliQ"
                     /locus_tag="ACHJ8C_002885"
     CDS             11759..12028
                     /gene="fliQ"
                     /locus_tag="ACHJ8C_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO5552710.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12037..12822
                     /gene="fliR"
                     /locus_tag="ACHJ8C_002886"
     CDS             12037..12822
                     /gene="fliR"
                     /locus_tag="ACHJ8C_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO5552711.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            13112..13735
                     /gene="rcsA"
                     /locus_tag="ACHJ8C_002887"
     CDS             13112..13735
                     /gene="rcsA"
                     /locus_tag="ACHJ8C_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO5552712.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASSSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(13779..13967)
                     /gene="dsrB"
                     /locus_tag="ACHJ8C_002888"
     CDS             complement(13779..13967)
                     /gene="dsrB"
                     /locus_tag="ACHJ8C_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO5552713.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            14130..14357
                     /gene="yodD"
                     /locus_tag="ACHJ8C_002889"
     CDS             14130..14357
                     /gene="yodD"
                     /locus_tag="ACHJ8C_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO5552714.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14655..15470
                     /gene="yedP"
                     /locus_tag="ACHJ8C_002890"
     CDS             14655..15470
                     /gene="yedP"
                     /locus_tag="ACHJ8C_002890"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO5552715.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLHEANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEICQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FIARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGRRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(15467..17161)
                     /gene="dgcQ"
                     /locus_tag="ACHJ8C_002891"
     CDS             complement(15467..17161)
                     /gene="dgcQ"
                     /locus_tag="ACHJ8C_002891"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO5552716.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQMHQHPFSVIQVDLDHFKAINDRFGHQ
                     VGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(17332..17514)
                     /gene="yodC"
                     /locus_tag="ACHJ8C_002892"
     CDS             complement(17332..17514)
                     /gene="yodC"
                     /locus_tag="ACHJ8C_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO5552717.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(17593..18510)
                     /gene="yedI"
                     /locus_tag="ACHJ8C_002893"
     CDS             complement(17593..18510)
                     /gene="yedI"
                     /locus_tag="ACHJ8C_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO5552718.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            18683..19603
                     /gene="yedA"
                     /locus_tag="ACHJ8C_002894"
     CDS             18683..19603
                     /gene="yedA"
                     /locus_tag="ACHJ8C_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO5552719.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(19592..20062)
                     /gene="vsr"
                     /locus_tag="ACHJ8C_002895"
     CDS             complement(19592..20062)
                     /gene="vsr"
                     /locus_tag="ACHJ8C_002895"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO5552720.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(20043..21461)
                     /gene="dcm"
                     /locus_tag="ACHJ8C_002896"
     CDS             complement(20043..21461)
                     /gene="dcm"
                     /locus_tag="ACHJ8C_002896"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO5552721.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(21528..22223)
                     /gene="yedJ"
                     /locus_tag="ACHJ8C_002897"
     CDS             complement(21528..22223)
                     /gene="yedJ"
                     /locus_tag="ACHJ8C_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO5552722.1"
                     /translation="MELQHWQAQFENWLKNHHQHQDAAHDLCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSMAKNHPQRQRSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGLALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLGAELAGENEG
                     IDHKVIDAFSPAG"
     gene            complement(22263..22544)
                     /gene="drpB"
                     /locus_tag="ACHJ8C_002898"
     CDS             complement(22263..22544)
                     /gene="drpB"
                     /locus_tag="ACHJ8C_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFO5552723.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         5550 a         5931 c         5751 g         5392 t
ORIGIN      
        1 atgaaagtag aacgcccatt gctaagtgtt cctgaatttg cgttgagaag cctcggaaaa
       61 tagatgtttt tagttgctcc caacaatata ttaactcggg gttctgaggt aagcagaaac
      121 ctgtcgtgtt acgaatttga tcagttatcg cataaaattt ttgaaacttt tcaattagat
      181 caatgggata ataaagcatt tctgcaagat aaagacctgc ttctggataa ttcgcaatat
      241 aaaattcata tccacaggga aataatatta tctgattatt atcaacagtt agactatgcg
      301 tctcccaatt gataactttt tttccctgac ggatacgaca taaagctggc ataagaggct
      361 taaccctgtg aatctcatga tgtttatgca tccgtatttc ttcaatcttt aggttagtct
      421 tatcttttgc cagcatactc ttaccctact ttatctcata aactggtgtt atctcagcgg
      481 ttgcaatttt attggcatta agcatataac caactaacgc tccgctggaa ttagaatcca
      541 caggaatctt atcagttttt agagcccata ctttaaactg gtaatgatgg ggtttatctc
      601 ctttgggagg acatgctcca ccaaatcctg cataaccaaa atcatttcgg ccttgaacag
      661 caccagtcgg aagttttgta ccatcacgtc ttcctgcatc agtgggcaaa tatgttacag
      721 ttgctggaat attagcaaca gtccagtgcc accatccact gcctgtagga gcatctggat
      781 catacacagt tacggcaaag cttttggtgc cttcaggagc accagaccag gttaatgagg
      841 gcgatgtatt accaccttca cacccaaatc cagaaaatac atgagacgtt gtaagttgct
      901 ctcctgtttt tatttcatta ctagtgacct gaaatgctgc ggcctgcgca gaaaatgtta
      961 tgaatgccaa tacacttgaa acgataagtt ttttcataaa aacctcattg ttgtgaccta
     1021 tcgttatttt atttgatgtg ccttgatctc attatgcata aaggcgcaat gatcgcgcaa
     1081 atgcaatcat aattataaag gtgccccgaa cattagccat aatatgcaca aatcagattc
     1141 ctgttatctc aaggatgatt attttttaat gcggtgcact tcgtgacaac aagttaatga
     1201 ctaacacacc ggcacaaatc aacatcatgc ctatgatagc tggcaggtcc agtcgttgac
     1261 caaaaaaaaa ccaggaaagc aaactaatca gaacgatacc gactcctgac cagatagcat
     1321 aagcaatccc tgtaggtata taagccagcg tctgagctaa taaccagaat gatgcacaat
     1381 agcaaataat tgtgccaaca gagggccata accgtgtaaa tccctctgaa aacttcatta
     1441 aggttgtacc aatgacctct gcaagtattg cgccaccaag ataaatataa gggttcatag
     1501 catattcttt cctgttcata ctggagagaa ttgtactaca gtttgaacac aactcaccgt
     1561 tttcatcctg gtgtatgtgt atccaggggg ctctggcgtg gcaagagata aaggcaggaa
     1621 caataatgat cagaaggaac cccaggctaa cgaaagagtt cactcttcct gggagcaaag
     1681 cgaagacaaa acatgttatc gcgtttacgg atcgtggatg gcagaaaaca accaggatca
     1741 ggtatccatt ttaaaccaga aattgagtga gcttgcccca tccatgcccc acgctgtggg
     1801 ttcggatgta attaaacaag catagtcaac aggttacaaa acctacactt gcatggacat
     1861 cacttcctga tacgccgcca ccagcttatt acgcacctga atccccattt gcatagaaac
     1921 tgaggctttt tgcatatcgg tcatcacatc gtttaacgcc acgccgggtt caccgagagt
     1981 gaatttttct gcctgcgtgc gggcggctgt ttgtgtatcg cttatgcgat cgagcgcggc
     2041 gtgcagctgc ccggcaaaac taatggtcgg ttgcggcagt gattcctgcg cacgcgcact
     2101 catcgccgta gcctgtaact ggctgataac cccttcaatc ccctgtatcg ctgacatttt
     2161 cgtctcccgg ataatttctg ttagcaaagc ctaccagtaa gtcaataaga caaaggcgct
     2221 aaatagcaac aaaaaaacgg gtttattggc ggatagaaaa aaacgaaagc acaaataatg
     2281 ggagcgtcaa tttttcgagt ttgctgaccc gggagtgagt cttgttccac tttgccaata
     2341 acgccgtcca taatcagcca cgaggtgcgc gatgaatgcg actgcagccc agacaaaatc
     2401 tcttgagtgg cttaatcgcc tgcgtgcgaa tccgaaaatt ccattgattg ttgccggttc
     2461 cgcagcagtg gcggtcatgg tcgcactgat cctgtgggcg aaagcccccg actaccgcac
     2521 attattcagc aatctttccg atcaggatgg tggcgcaatt gtcagccaac tgacgcaaat
     2581 gaatattcct taccgcttca gcgaagccag cggcgctatt gaggttccgg cagataaagt
     2641 tcacgaactg cgcctgcgcc tggcgcaaca aggtttgccc aaaggcggcg cggtcggttt
     2701 cgaactgctc gatcaggaaa agtttggtat cagccagttc agcgaacagg tgaattatca
     2761 gcgggcgctg gaaggcgagc tttctcgtac catcgaaact atcggcccgg taaaaggggc
     2821 gcgcgtacat ctggcaatgc cgaaaccgtc tttattcgtc cgtgaacaaa aatccccttc
     2881 tgcatcggtg acggtaaatc tgttacccgg ccgcgcactc gatgaagggc aaattagcgc
     2941 tattgtacat ctggtttcca gcgcggttgc tggtctgccg cctggaaacg tcacgctggt
     3001 ggatcagggc gggcatctgt taacccagtc caataccagc gggcgcgatc ttaatgacgc
     3061 tcagttgaaa tatgccagcg atgtcgaagg ccgtattcag cggcgtattg aagcgatcct
     3121 gtcgcctatt gttggtaacg gtaatattca cgcccaggtc acggcgcaac tggacttcgc
     3181 cagtaaagaa caaacggaag aacagtatcg ccctaacggt gatgaatctc atgcggcgct
     3241 tcgttcacgc cagcttaatg agagcgagca aagcggttcc ggttatccgg gcggcgtacc
     3301 gggagcgttg tcgaatcaac cggcacctgc gaataacgcg ccaatcagca cgcctccggc
     3361 aaatcaaaat aaccgccagc agcaggcgag caccaccagc aatagtgggc cgcgtagcac
     3421 acagcggaat gaaaccagta actacgaagt cgatcgcacc attcgtcata ccaaaatgaa
     3481 cgtgggcgat gtgcaacgtc tgtcagtcgc ggtagtggtg aattacaaaa ccttgccagg
     3541 cggtaaacca ttgcctctca gcaacgaaca gatgaagcaa attgaagctc tgacccgcga
     3601 ggcgatgggc ttttctgaaa aacgcggtga ctcgctcaat gtcgtcaact cgccgttcaa
     3661 tagcagtgac gaaagcggcg gagcactgcc gttctggcaa cagcaagtgt ttatcgatca
     3721 gttgctggct gccggtcgct ggttgctggt actgctggtg gcgtggttgc tgtggcggaa
     3781 agcggtacgc ccgcagctaa cacgtcgcgc cgaggcggtg aagactgtgc agcaacaggc
     3841 gcaggcccgc gaggaagtgg aagatgcggt ggaagtccgt ctgagcaaag acgaacaact
     3901 ccaacaacgg cgcgctaacc aacgactggg ggcagaagtc atgagccagc gtatccgtga
     3961 aatgtctgat aacgatccgc gcgtggtggc gctggtcatt cgccagtgga taaataacga
     4021 tcatgagtaa cctgacaggc accgataaaa gcgtcatcct gctgatgacc attggcgaag
     4081 accgggcggc agaggtgttc aagcacctct cccagcgcga agtgcaaacc ctgagcgctg
     4141 caatggcgaa cgtcacgcag atctccaaca agcagctaac cgatgtgctg gcggagtttg
     4201 agcaagaagc tgaacagttt gctgcactga atatcaacgc caacgattat ctgcgttcgg
     4261 tattggtcaa agctctgggt gaagaacgtg ccgccagcct gctggaagat attctcgaaa
     4321 cccgcgatac cgccagcggt attgaaacgc tcaactttat ggagccgcag agcgccgccg
     4381 atctgattcg cgatgagcat ccgcaaatta tcgccaccat tctggtgcat ctgaagcgcg
     4441 cccaggccgc cgatattctg gcgttgttcg atgaacgtct gcgccacgac gtgatgttgc
     4501 gtatcgccac ctttggcggc gtgcagccag ccgcgctggc cgaactgacc gaagtactga
     4561 atggcttgct cgacggtcag aatctcaagc gcagcaaaat gggcggcgtg agaacggcag
     4621 ccgaaattat caacctgatg aaaacgcagc aggaagaagc cgttattacc gccgtgcgtg
     4681 aattcgacgg cgagctggcg cagaaaatca tcgacgagat gttcctgttc gagaatctgg
     4741 tggatgtcga tgatcgcagc attcagcgtc tgttgcagga agtggattcc gaatcgctgc
     4801 tgatcgcgct gaaaggggcc gagcagccac tgcgcgagaa attcctgcgc aatatgtcgc
     4861 agcgtgccgc cgatattctg cgcgacgatc tcgccaaccg tggtccggtg cgtctgtcgc
     4921 aggtggaaaa cgaacagaaa gcgattctgc tgattgtgcg ccgtcttgcc gaaactggcg
     4981 agatggtgat tggcagcggc gaggatacct atgtctgata atctgccgtg gaaaacctgg
     5041 acgccggacg atctcgcgcc accaccggca gagtttgtgc caatggccga gtcggaagaa
     5101 accattattg aagaggtcga acccagcctt gagcagcaac tggcgcaact gcaaatgcag
     5161 gcccacgagc agggttatca ggcggggatt gccgaaggtc gccagcaagg tcacgaacag
     5221 ggctatcagg aaggactggc ccaggggctg gagcaaggtc tggcagaggc gaaggctcaa
     5281 caagcgccaa ttcatgcccg gatacagcaa ctggtcagcg aatttcaaac tacccttgat
     5341 gcgcttgata gtgtgatagc ttcgcgcctg atgcagatgg cgctggaggc agcacgtcag
     5401 gtcataggtc agacgccaac ggtggataac tcggcactga tcaaacagat ccaacagttg
     5461 ttgcagcaag aaccgttatt cagcgggaaa ccacagctgc gcgtacaccc ggatgatctg
     5521 caacgtgtgg atgatatgct cggcgcaact ttaagtttgc atggctggcg cttgcggggc
     5581 gatcccaccc tccaccctgg cggctgtaaa gtctccgccg atgaaggcga tctcgacgcc
     5641 agtgtcgcca ctcgctggca agaactctgc cgtctggcag caccaggagt ggtgtaatga
     5701 ccacgcgcct gactcgctgg ctaaccacgc tggataactt tgaagccaaa atggcgcagt
     5761 tgcctgcggt acgtcgctac gggcgattaa cccgcgctac cgggctggtg ctggaagcca
     5821 ccggattaca attgccgctc ggcgcaacct gtgtcattga gcgccagaac ggcagcgaaa
     5881 cgcacgaagt agaaagcgaa gtcgttggct ttaacggtca acggctgttt ttaatgccgc
     5941 tggaggaagt ggaaggtgtc ctgcccggcg cgcgtgtata tgccaaaaac atttcggcag
     6001 aagggctgca aagcggcaag cagttgccgc tcggtccggc gttattaggt cgtgttctgg
     6061 acggtagcgg taaaccgctc gatggcctgc ccttccccga tacgacggaa accggtgcgc
     6121 tgattacccc gccatttaac ccgttgcaac gtacaccgat tgaacatgtg ctggacaccg
     6181 gcgtgcgccc aatcaatgcc ctgcttaccg tcggacgcgg gcagcgtatg gggctgtttg
     6241 ccgggtccgg cgttgggaaa agtgtgctgc tggggatgat ggcacgttac acccgcgccg
     6301 atgtcattgt cgtgggtttg attggtgaac gtgggcgcga agtaaaagat tttattgaga
     6361 atatcctcgg tgccgaaggg cgtgcacgct cagtggtgat tgccgctccg gcggatgttt
     6421 ctccgctcct gcgaatgcaa ggtgccgcct atgccacgcg cattgccgaa gattttcgcg
     6481 atcgtggtca gcatgtgttg ctgattatgg actccctcac ccgctacgcg atggcccagc
     6541 gtgaaattgc gctggcgatt ggcgaaccac ctgccactaa aggttatcca ccgtcggtgt
     6601 ttgccaaatt accggcactg gtcgagcgtg ccggtaatgg cattagcggc ggcggctcga
     6661 ttaccgcgtt ttataccgtg ctcaccgaag gcgatgacca gcaggacccc attgctgact
     6721 ccgcgcgggc aatcctcgac ggtcacattg tgctgtctcg ccgactggcg gaagccgggc
     6781 actatccggc tatcgatatt gaagcgtcga tcagtcgcgc aatgacggcg ttgatcagtg
     6841 agcaacatta cgcgcgagtg cgcaccttca aacagctgtt gtcgagtttt cagcgtaacc
     6901 gcgatctggt tagcgtcggc gcgtatgcca aaggcagcga tccgatgctc gacaaagcca
     6961 tcgccctgtg gccgcagctg gagggctatt tgcaacaagg catttttgaa cgcgcggact
     7021 gggaagcgtc tctccagggg ctggagcgta ttttcccgac agtatcatag cccaggagat
     7081 aacggcagat ggcagaacat ggtgcgctgg cgaccctgaa agatctggca gaaaaagagg
     7141 tagaggatgc agctcgcctg ctgggtgaaa tgcgtcgcgg atgtcagcag gcggaagaac
     7201 agctcaaaat gctgatcgat tatcagaatg aatatcgcaa taacctcaat agcgatatga
     7261 gtgccgggat gaccagcaac cgctggatca actatcagca gtttatccag acgctggaaa
     7321 aagccattac tcagcatcgc cagcaactta atcagtggac gcagaaagtt gacattgccc
     7381 tgaacagctg gcgagaaaag aaacaacgtt tgcaggcctg gcagacactg caggaacggc
     7441 aatccacggc ggcactgctt gcagaaaacc gcctcgatca gaaaaagatg gatgagttcg
     7501 cccagcgcgc cgccatgagg aaacctgaat gattcgctta gcgcccttga ttaccgccga
     7561 cgttgacacc accacattgc ctggcggcaa agccagcgat gctgcacaag attttctcgc
     7621 gttgttgagc gaagcattag caggcgagac aacaaccgac aaagcggccc cccagttgct
     7681 ggtggcaaca gataagccca cgacaaaagg cgagccgctg gtcagcgata ttgtttccga
     7741 cgcgcaacaa gcggatttac tgatccctgt ggatgaaaca ctgcctgtca tcaacgacga
     7801 acaatccaca tcaacaccgt taaccaccgc tcagacgatg acgttggctg cggtggctga
     7861 caaaaatacg acaaaagacg aaaaagcgga tgatcttaat gaagacgtca ccgcaagtct
     7921 gagcgccctt tttgcgatgt tgccgggttt tgacaatacg cccaaagtga ctgatgcacc
     7981 gtcaaccgtg ttaccggcag agaaaccaac gttcttcaca aaactgactt ctgcgcaact
     8041 cactacagca cagcctgatg acgcccccgg cacaccagct cagccattat caccgctggt
     8101 agcagaagcc cagagtaaag cggaagtcat cagcacgcct tcgccggtga ccgctgccgc
     8161 cagcccgcta atcactccac accagacaca gccactgccc accgtcgccg cgcctgtttt
     8221 gagtgcaccg ctgggttctc acgaatggca acaatcatta agccagcata tttcgctgtt
     8281 cacccgccag gggcaacaaa gtgcagagtt gcgtctgcac ccacaggatt taggtgaagt
     8341 gcaaatttcc ctcaaagtgg atgataacca ggcgcagatc cagatgattt caccgcatca
     8401 gcacgtacgc gccgccctgg aagcagcgct gccggtactg cgtacgcagc tggccgaaag
     8461 tggcattcag ttagggcaaa gcaacattag tggcgaaagc tttagtggtc agcagcaggc
     8521 cgcttcccag caacagcaaa gccaacgcac agcaaaccat gaacctctgg cgggggaaga
     8581 cgacgatacg cttccggttc ccgtctcttt acaagggcgt gtaacaggta acagcggcgt
     8641 tgatattttc gcctaacgtc agaggtagca ccgtaatccg cgtcttttcc ccgctttgtt
     8701 gcgctcaaga cgcaggataa ttagccgata agcagtagcg acacaggaag accgcaacac
     8761 atgactgatt acgcgataag caagaaaagc aagcgatcgc tttggatccc gattctggta
     8821 ttcattaccc tcgcggcctg tgccagcgca ggttacagct actggcattc gcatcaggtt
     8881 gccgctgacg acaaagcgca gcaacgcgtc gtgccctccc cggtcttcta tgcgctggat
     8941 acctttacgg tcaatttggg cgatgcggat cgcgtgcttt atatcggcat aaccctgcga
     9001 ctgaaagatg aagcaacccg ctcgcggctg agtgagtatt tgccggaagt ccgtagtcgc
     9061 ttgctgttac tgttttcgcg tcaggatgct gccgtactgg cgacagaaga aggcaagaaa
     9121 aacctgattg ccgagattaa aaccacactt tccaccccgc ttgttgccgg gcaaccgaaa
     9181 caggatgtca ccgacgtgct gtataccgct tttattctgc gataacgaca tgggcgatag
     9241 tattctttct caagctgaaa ttgatgcgct gttgaatggt gacagcgaag tcaaagacga
     9301 accgacagcc agtgttagcg gcgaaagtga cattcgtccg tacgatccga atacccaacg
     9361 acgggttgtg cgcgaacgtt tgcaggcgct ggaaatcatt aatgagcgct ttgcccgcca
     9421 ttttcgtatg gggctgttca acctgctgcg ccgtagcccg gacattaccg tcggggccat
     9481 ccgcattcag ccgtaccatg aatttgcccg caacctgccg gtgccgacca acctgaacct
     9541 tatccacctt aaaccgctgc gcggcacagg gctggtggtg ttctcgccga gcctggtgtt
     9601 tatcgccgtg gataacctgt ttggcggcga tgggcgcttc ccaaccaaag tggaaggtcg
     9661 cgagtttacc cataccgaac agcgcgtcat caaccgcatg ttaaagctgg cgctggaagg
     9721 ctatagcgac gcctggaaag cgattaatcc gctggaagtt gagtacgtgc gttcggaaat
     9781 gcaggtgaaa tttaccaata tcaccacctc gccgaacgac attgtggtta acacgccgtt
     9841 ccatgtggag attggcaacc tgaccggcga atttaatatc tgcctgccat tcagcatgat
     9901 cgagccgcta cgggaattgt tggttaaccc gccgctggaa aactcgcgta atgaagatca
     9961 gaactggcgc gataacctgg tgcgccaggt gcagcattca cagctggaac tggtggccaa
    10021 ctttgccgat atctcgctac gcctgtcgca gattttaaaa ctgaaacccg gcgacgtcct
    10081 gccgatagaa aaacccgatc gcatcatcgc ccatgttgac ggcgtcccgg ttctgaccag
    10141 tcagtacggc actctcaacg gtcagtatgc gttacggata gaacatttga ttaacccgat
    10201 tttaaattct ctgaacgagg aacagcccaa atgagtgaca tgaataatcc ggccgatgac
    10261 aacaacggcg caatggacga tctgtgggct gaagcgttga gcgagcaaaa atcaaccagc
    10321 ggaaaaagcg ctgccgacgc ggtgttccag caatttggcg gtggtgatgt cagcggaacg
    10381 ttgcaggata tcgacctgat tatggatatt ccggtcaagc tgaccgtcga gctgggccgt
    10441 acgcggatga ccatcaaaga gctgttgcgt ctgacgcaag ggtcagtcgt agcgctggac
    10501 ggtctggcgg gcgaaccgct ggatattctg atcaacggtt atttaatcgc ccagggcgaa
    10561 gtggtggttg ttgccgataa atatggcgtg cggatcaccg atatcattac cccgtccgag
    10621 cggatgcgcc gcctgagccg ttagtgatga ataaccatgc tactgtgcaa tcttccgcgc
    10681 cggtttctgc tgcgccactg ctgcaggtga gcggcgcact catcgccatt attgccctga
    10741 tcctcgctgc cgcctggctg gtaaaacggc tgggatttgc ccctaaacgc actggcgtta
    10801 acggtctgaa aattagtgcc agtgcttcgc tgggcgcgcg tgaacgggtc gtgatggtcg
    10861 atgtggaaga tgcacggctg gtgctcggcg ttaccgcagg gcaaatcaat ctgctgcata
    10921 aacttccccc ttcagcacca acggacgaga taccgcagac tgattttcag tcggtcatga
    10981 aaaatttgct taagcgtggc gggagatcct gatgcgtcgt ttgttttctg tcgcaccggt
    11041 ccttctctgg ctgattacgc ccctcgcctt cgcgcaactg ccgggtatca ccagccagcc
    11101 gctacctggc ggtggacaaa gctggtcgct cccggtgcag acgctggtgt tcatcacctc
    11161 gttgacgttc attccggcaa ttttactgat gatgaccagt ttcacccgca tcattattgt
    11221 ttttggttta ttgcgtaacg cgctgggaac gccctccgcg ccacctaacc aggtattgct
    11281 ggggctggca ctgtttttga ccttttttat tatgtcgcca gtgatcgaca aaatttatgt
    11341 cgatgcgtac cagccattca gcgaagagaa aatctcaatg caggaggcgc tggaaaaagg
    11401 ggcgcagccg ctgcgtgaat ttatgctgcg tcagacccgc gaggcggatt tagggttgtt
    11461 tgccagactg gcgaataccg gcccgttgca gggacctgaa gctgtgccga tgcgcatttt
    11521 gctcccggcc tacgtgacca gcgagttgaa aactgcattt cagataggct tcacgatttt
    11581 catccctttt ttgattatcg acctggtgat tgccagcgtg ttgatggcat tggggatgat
    11641 gatggttccc cctgccacca ttgctctgcc ctttaaactg atgctgtttg tactggtgga
    11701 tggctggcaa ttgctggtcg gttcgctggc gcagagcttt tacagctaga gaggcaaaat
    11761 gacacctgaa tcggtcatga tgatgggaac tgaagcgatg aaagtcgcgc tggcactggc
    11821 tgccccgctg ttgctggtgg cgctggtcac gggtcttatc atcagtattt tgcaggccgc
    11881 cacgcagatc aacgaaatga cgctgtcgtt tattccaaaa atcatcgccg tatttatcgc
    11941 cattattatt gccggaccgt ggatgctcaa tctgttgctg gattacgtcc gcaccttgtt
    12001 cactaacctg ccgtatatca tcgggtaggc cgtacaatga tgcaggtaac aagcgatcaa
    12061 tggctttcct ggttaagcct gtacttctgg ccgttactgc gcgtgctggc gctcatttct
    12121 accgcaccaa ttctgagcga acgcagcgtg ccgaaacggg taaaactggg tctggcaatg
    12181 atgatcacgt tcgctattgc cccatcatta cctgccaacg atgttcctgt tttttcgttc
    12241 tttgctctgt ggctggccgt gcagcagatc ctgatcggca ttgcgcttgg ttttaccatg
    12301 caatttgcct ttgccgcggt gcgaaccgct ggtgaaatta tcggtctgca aatggggctg
    12361 tcatttgcga cgtttgtcga tccggccagc catcttaata tgcccgtttt agcgcgtatc
    12421 atggatatgc tggcgttact gctgttcctg acatttaacg gtcatttatg gttgatttca
    12481 ctgctggtcg atacctttca caccctgcca attggtggcg aaccgttgaa cagcaatgcg
    12541 tttctggcac tcaccaaagc agggagtttg attttcctta atgggctgat gctggcgtta
    12601 ccgctcatta ctctgctgct gaccctgaat ctggcattag gtttactgaa tcgtatggcc
    12661 ccgcaattat ccatttttgt tattggattt ccattaactc tgactgtcgg tatctcttta
    12721 atggcggcat taatgccgtt aattgcgcct ttttgcgaac atttattcag tgagatgttt
    12781 aatttgctgg ctgatattat tagtgaattg ccattaatat aattccgtaa cgtttatcat
    12841 gctaccctaa ggattatccg aaaaataata cctacgaaca tcttccagga tactcctgcg
    12901 gcgaaatatt tgttttaagc tcactcacat atcgcaacat ttactttact ttaagacaat
    12961 tccaggcaaa ttatacaaca ctttacggga tagtaagtcc gcctgaaaaa ttgcgagagt
    13021 ggcgcattag gtgacccatg ttgttccgtt tagtcatgat gaaatattca ggtaagggga
    13081 attatcgtta cgcattgagt gagggtatgc catgtcaacg attattatgg atttatgtag
    13141 ttacacccga ctaggtttaa ccgggtatct gttgagtaga ggggttaaaa aaagagaaat
    13201 caacgacatt gaaaccgttg atgaccttgc catagcttgt gattcacagc gcccttcagt
    13261 ggtgtttatt aatgaggact gtttcatcca cgatgcttct agcagtcagc gcatcaagca
    13321 catcattaat caacatccca atacgttatt tatcgttttt atggcaattg ccaatgttca
    13381 ttttgatgaa tatctattgg tcagaaaaaa tttattgatc agttctaaat cgattaaacc
    13441 ggaatctctc gacgatattc ttggcgatat tctgaaaaaa gagacaacga taacctcgtt
    13501 tttaaatatg ccgacgttat cattgagccg aaccgaatcg agtatgttgc gaatgtggat
    13561 ggcaggtcag ggaaccattc aaatctccga ccaaatgaat atcaaagcca agaccgtttc
    13621 atcgcataaa ggtaatatta aacgtaagat caaaacgcat aataaacagg ttatctacca
    13681 tgtcgtccga ctgacggata atgtgactaa tggtattttt gtcaacatgc gctaacacat
    13741 tctgactggt ggtttcccac cagtcaggct gaataagatt actctgcttt ctcaacaaag
    13801 ataccgtcct gatgccctgc ttcattaaag aaccagatgc cgagcgggta gtcttccagc
    13861 gaaaccaggt acattgtgcc ttcactaaac tcctcaactg ccagtaccac gccaggacga
    13921 cgcggaccgc catccgtttt gactgttacc cgatcattca ccttcatcgt tttcctcctg
    13981 tggctttgtg ccagtgtaga acaatttcgt tttcctggca gcgccaggcg cgcgcgagtg
    14041 ctgattttct cgacggtcta tacttaagag atgccaggcg gacttaacaa ctggcggcaa
    14101 caacagagta acggttgcga ggaaagatga tgaaaaccgc aaaagagtac agcgataccg
    14161 caaaacgtga ggtcagcgtc gatgtcgatg ccctgctggc ggcgatcaat gaaattagcg
    14221 aaagcgaagt tcatcgcagc cagaacgatt ctgaacacgt tagcgtcgat ggacgtgaat
    14281 atcatacatg gcgtgaattg gcggatgcct tcgaactgga tatccatgac ttcagcgtct
    14341 ctgaagtgaa tcgttgaatg cacaataaaa aaatcccgac cctgaggggg tcgggatgaa
    14401 acttgcttaa gcaagaagca cttaaaaaat tcgttacacc aggaaatctg atgtgttcat
    14461 caccttatct gcaatttttt tcgctgacaa gaaaatattc gcgctatgaa tgattaacgc
    14521 tacttatgaa tagaaatgtg acgcgcgtca ccccttggtg gctctggtgt caacgggatc
    14581 gctcccgaaa aaatcatctt tgattatttg tgctgttttg agaatcatcc tggtaagggg
    14641 agtactgcgc gaccatgttt tcaattcaac aaccactact ggtttttagc gatcttgatg
    14701 gcaccctgct ggacagtcat agttatgact ggcaaccggc agccccctgg ctcagccgtt
    14761 tacacgaagc caatgttccc gtcattctct gtagcagtaa aacttcagcg gaaatgctgt
    14821 acttgcaaaa aatgttgggg ctacaaggct taccgctgat tgcagagaat ggcgcagtga
    14881 tccagcttgc tgaacaatgg caggatatag acggttttcc acgcatcatc tcaggtatta
    14941 gccatggcga aatctgccag gttttaaata cgctacgcga gaaagaacat tttaaattca
    15001 cgacttttga tgatgtcgac gatgcaacca ttgccgaatg gacgggatta agccgtagcc
    15061 aggcggcgct gacgcagctg catgaggcgt cggtaacgct aatctggcgc gacagtgacg
    15121 agcgtatggc acaatttatc gctcgtctga acgaactggg cttgcagttt atgcagggtg
    15181 cgcgcttctg gcacgtactg gatgcctctg ccggaaaaga tcaggctgcc aactggatta
    15241 tcgcgaccta tcaacaattg tcaggcagac gcccaaccac acttggcctg ggcgatgggc
    15301 caaacgatgc gcctttactg gaggtaatgg attacgcggt gattgtgaaa gggctaaacc
    15361 gtgaaggggt acatctgcat gatgaggatc cggcccgcgt ctggcgaacg cagcgtgaag
    15421 gaccggaagg ctggcgtgaa gggctggacc attttttctc cgcccgttaa gcgttatcgc
    15481 tcgcgaatac ccgattgcgc ccggcctgtt ttgcgaggta aagccgacgg tcggccaatg
    15541 actggagttg ttcaaaatca taatcaccgg tttcctcgct gctgcttacc cccagcgagg
    15601 cactgatgcg tatcgttgta ctcttggcga tcagcatctc tttttcattt aacttcaggc
    15661 gaatacgttc tgcgacctcc gcagcctgcg tcagattcgc gcctggcaga atcacacaaa
    15721 actcctcacc accgacccgc ccggcaacgt cctgcgcacg caaggaactg ctaattaatc
    15781 cggcagcatg agaaagaaca cggtcgccca cctgatgacc aaagcggtca ttaatcgctt
    15841 taaaatggtc aagatcgacc tgaatgacag aaaatggatg ttggtgcatc tgacacaatt
    15901 tagcgagcgg acgggctttt tcgaacagtg cgccacggtt atataaacgc gttaaggtgt
    15961 cgtgccacgc ctgccactgc aacgagcttt gcagaacata catgttgctg accatccggc
    16021 gaatcacata ccaggagagg agtaacatgg tggtaaagag cgcccacagc agggttaatg
    16081 caatgctgat actgccgaaa tcgccgcgca cgccttcgct tagcgtatgg acgcgcacca
    16141 gcacaccgtc gaaatggtcc agacgttccc agctaacata gcgactgtcc atacgaatgc
    16201 cgccccgcgt gtcatgttcc attgcctgcg ccagcaaggc cagttcacga ggatcaaaaa
    16261 tattccctgt tggatggtca ggattggaag aggtcaaaaa tctcagctta ctgtcataga
    16321 gctgatactc accatcgagg tttttatcga tggcgtttct taaaaattgc tgcatagtac
    16381 gcacgggaat actcatcccc agcacgccat accagtagtt attactgtct accggaacac
    16441 tgacggtaac ctgcggttca gtattgctgg cgtgttccgg ttgcgaagtg aaccagcgta
    16501 ccgcgcggtg acgattttct cgttgcgaat ggccgataaa ccagggttgg gtgacatagc
    16561 cgtaataacg cgttggtaca ttgcgcgtaa acaaggtcgg ctgcgtcgaa acgtaaaatc
    16621 cagcacgcga gacatacatc gcctgttcaa ccatcgacga ggagttgtgc gccagtcgca
    16681 gcaagtaacc aacttccagt gcagcggtaa tttcattgtc gaggctttca ttttcgcgag
    16741 acaggagatt cccctcgctg actaatgcat ccgatacgcc attgactggc agggtgcgcc
    16801 gtcggttgag ttcgatttgc caggcgtgct cgtcgcgatg ctgttcgaac tcggttacgg
    16861 cattacgcag agaggtgaaa tccagtggtg ccacgagagc ttcgcgcatg ccattacgca
    16921 aaaagatcag tttgtcgacg ttatactgca aatgcttatc gagcgcgttg gctacgtttt
    16981 ccagatgatt acgctggctg gagatatagg catcttccag caccaccact tcacgccagg
    17041 tgagcaaggt ggaaaaaagc aataccacga taaagcagag attaacgaca tgaccaggcc
    17101 ccaggcggcg tgcgagtttt ttcaaccagc tctggttttc catttttgtc tcgtgctgca
    17161 ccctgacgac tcccaacctg cttttttatg attctggcat aacccggcga tagcgtaccg
    17221 caaaacagcg ataattttat aatggtggca tgataagacg tgactatcat tcattgcagg
    17281 cattgggaca ccaggccgga tgtgatccgg cctggtgtta atgataccct ttcaaacttc
    17341 ttccgcagaa cgactcccct cccccggaac aagctcggtt tcatgaaaag cctcccgctt
    17401 gaccccgtaa ccgtcatacc aacgacactc aaccataccg ctggagtatc cagtgacaat
    17461 catccgcggg ccgccctctt taaccgtaac ttcctcacta accataaagc tcatactcgc
    17521 ctcctttttt cgagtgaaac ttgttcacct tagttgaaga tggcgaattt tgcataatag
    17581 ccagtgcgag tattaatgtg cctgaccgcg cattttcgct actgctttca ctcccagcac
    17641 cacgatgccg ccgatgataa atccaagaat cagatttaaa acagtcggta atatcatcgc
    17701 caccactgca ctttgctgcc cggcgatatg ttcaatagcg tgatgcagcg gcgcaatacc
    17761 atgtaccaca atcccgccgc cgacgaggaa catcgccagc gtgccgacaa tcgatagcgc
    17821 tttcatcagc cagggcgcaa taatcaataa ccctttacct aatgcctgca tcagcgcgct
    17881 ggatttttcc gccagccaat accccaggtc atcaatctta acgataaccc ctaccagacc
    17941 gtagacgccc acggtcacta ccagcgcgat gcctgaaagc accagcacct gattaagcaa
    18001 cggtgcctct gccacaatcc ccagcgtgat ggcgacgatt tccgcagaca aaataaaatc
    18061 ggtacgaatc gcccctttaa ttttgtcctt ttcaaacttc agcggatcct gcgccgccag
    18121 cttctccaga cgctgctggc tctgtgccgg atcttcttta tgtttacgcg cctccagcat
    18181 atgcagcact ttctctaccc cttcaaagca gagaaacgcg ccaccaatca tcaacagtgg
    18241 cgtaatcgcc cacgggataa acgcactgat gatcaacgcc agcggcacca gaatcacttt
    18301 attgatcagc gatcctttcg ccacgcccca gaccacggga agttcccggt tggcccgcac
    18361 gcctgaaact tgttgcgcat tgagcgataa atcatcccct aatacaccgg cggttttctt
    18421 cgccgccagt ttgcccatca cggagatatc gtccagcagt gtggcgatat catcgagcaa
    18481 cgtcagtaaa ctactcccgg ccaaaagcat tccctctcat ttttattgtt gaagggtgta
    18541 agtatgaagc aaaaacggca gctctgatac ttgcttcaca ggtcaacatt tatttaacaa
    18601 tcaagtgcaa ttatatctct cgccagcaga atgattttca cgtttactat gaaccacctt
    18661 tttatttccg ccgtgaggga ttatgcgttt ccgccagttg ttaccgcttt ttggcgcgct
    18721 gtttgcgttg tatatcattt ggggctcaac ctattttgtc attcggattg gcgtggaaag
    18781 ctggcctccg ttaatgatgg cgggcgttcg attcctggca gccggtattt tattgatggc
    18841 atttttgcta ctgcgcggac acaaacttcc cccgctacgt ccgctgctca atgccgcgct
    18901 gattggcctg ttattgctgg ctgtcggtaa tggcatggtg acggttgccg aacatcaaaa
    18961 tgttccttcc ggcatcgccg ccgtagtggt tgcaaccgtg cccctcttta ccctgtgctt
    19021 cagccgcctg tttggcatta aaacgcgcaa actggaatgg gtgggtatcg ccattgggct
    19081 tgccggaatc atcatgctca atagcggtgg aaatttaagc ggcaatccgt ggggcgcgat
    19141 tctgatttta atcggctcga ttagctgggc gtttggctca gtttatggct cgcgcattac
    19201 cttacctgta gggatgatgg cgggtgcgat tgagatgctg gcggcaggcg tggtgttaat
    19261 gatcgcatcg atgattgcgg gtgaaaaact gacggcgctc ccttccctgt caggcttcct
    19321 tgcggtcggc tatctggcgc tgtttggttc gattatcgcc attaacgctt atatgtattt
    19381 aatccgtaat gtcagtccgg ctctcgccac cagctacgcc tacgttaacc cggtggtcgc
    19441 ggtcttgctg ggtacgggac tgggtggcga aacgctgtcg aagattgaat ggctggcgct
    19501 cggcgtaatt gttttcgcgg tggtactggt cacgttggga aaatatctct tcccggccaa
    19561 acccgttgtc gcgccagtta ttcaggatgc atcaagcgag taaatgaatc ccctgcgtgt
    19621 cgatctgcgc gctggcacct tcgccgcaga tccactcttc cagacgctcg gtaagcgccg
    19681 catccgtcag cttctcgcgc ccacgtaacg cacactccca gacaatcagt acgcgccagc
    19741 cgagctcctg caagcgactg atatcgcggc gatcgcgttc aacattttta cctatcttct
    19801 ccagccagaa ctcagttcga gtcgcaggca ctttaaacag atagcagtga tgatgatgcc
    19861 agaagcagcc gtgggtaaat atcacgcagc gatattcatc aacgacaaaa tccgggcgtc
    19921 cgggcagact ggcgtcctga acgcgaaacg ccaggccctg cccggttaac aaactggcga
    19981 ggcgcttctc aatcgccgta tcacgcgtgg caatcgcgcg catatttttg ctgcgggtgg
    20041 ccttatcgtg aacgtcgacc atgttgtgcc tcttgctgac gcaacgccac cgcctgtttg
    20101 atttttggct caagcagttt tgccaccgcg gcaaagaccg gtacgaccac cgagttaccg
    20161 aactggcgat aggcctgagt gtccgaaacc ggaatgcgga atttcgcttc tcccggcgct
    20221 tcaaaaccca ttaagcgcgc acattcacga ggcgttaacc gacgtggacg atgttgctga
    20281 ttcagcggat cgtcaaagtc tttctcaccc gtggccatat cccaaccgcg atcgattaaa
    20341 atttccgcgc catctttgta ataacgagca gacagcgtac gcgtgacgct ttgcggattg
    20401 ttcggataaa ccattccata accgaagccg ttaccgcgtg cctgatgttt tttcgcataa
    20461 cgatagaggt acttccacag caccggcgtc aggatatatt tcgcctcgac catcgggtcc
    20521 agcagctgcg ccagcgtcac tcgctgtgca gggaaacatt cgctgatatc acgcagagta
    20581 aaatcggctt tcagattcag atcgcgacga aaacccacca gcacgatgcg ttcacggtgc
    20641 tgcggcagga aatgtttgcc gtcgatgatt ttcggatcgt ccggcccgtt atcttctgca
    20701 tcagccacgt catagcccag ttcgtccagc gtctgcatga tgatgcggaa cgtttttccc
    20761 ttatcgtgac tcttcaggtt tttgacattt tcgagcacaa acatcgccgg acgacgcgcg
    20821 tcgataatgc gcaccacatc aaagaacagc gtgccctggg tatcgcaggc aaaaccgtgc
    20881 gcccgcccga gcgagttctt tttcgatacg ccagccagcg aaaatggctg acaagggaaa
    20941 ccggccagta aaacatcgtg ttcaggaatg tgttgacgaa tatgttccgc cgccgcctca
    21001 tcactcacgc cttcttgatg actgagggtg atgtcgcgga tatcttcatt aaaatgatgc
    21061 gtcgccgggt cgcaataatg gttggcttta taagtgcgta ccgcatgttt gttccattcg
    21121 ctggtaaaca cgcactgtcc accaatcgat tcaaaaccgc gacggatgcc gccaattccg
    21181 gcaaatagat cgataaagcg aaacgcataa tgcggatgat gttccggtgg tttgggtaac
    21241 aacgtttgca gatgggcaaa ctctttctca cttaaacggt gccatgccga gtcattcgcc
    21301 agcgcacgtt ttaaaattgc cgcgctccag tgattctcac ccacaccatt aagctgcgcc
    21361 accagcgttt taacatcata aatttgcagc agtttctcca gcattgcctg tgcggcattc
    21421 ccggtgctgt atgaatcggt tactgatata ttttcctgca tagatttaac cggccatctg
    21481 agatggccga caggttaaca taattactgc cgtttagggc cacgcgctca gccagcgggt
    21541 gaaaacgcat ctatcacctt gtgatcgata ccttcattct cccccgccag ttcggcaccg
    21601 agcttcgcca taaactcgac taaaaattgc gcgttgtgct gtgccagctg cttgcctctt
    21661 gcggtttgca tggtttgcgg taatttcagt aactttgtct ggaaatgatc cagcgcgtag
    21721 cgtttatcat caagcgggcg atgctgtgcg aacggatctt cgccatcaaa cagcgccaag
    21781 cccaatgccc ctgaaacggc aaatacgcgc gccaggccaa tcgcccccag ggcttccagc
    21841 cgatccgcat cctggacgat tttagcttcc gttgttaagg gggcgatttg cgcgctgaaa
    21901 ctgtgagcgg caatggcatg acaaacggcc tcaattttct ccgccggaaa ttgcacgaac
    21961 tcttcacgga gcaggcgacg cgtctcttct gccgccagga ttgaagaacg ctgccgttgc
    22021 ggatgatttt tcgccatgct cacgatatcg tgaaaataac aggcggttaa aatcaccagc
    22081 atatcgacat cgtcatctgc cgccagtttc tgtgccgtag cccagacacg gcgaaaatga
    22141 cacaggtcat gcgccgcgtc ctgatgttga tgatggtttt tcaaccagtt ctcgaactgt
    22201 gcctgccagt gttgtaactc catacgcgct ccgttgccaa agagtcgcaa ccttagcagt
    22261 ttttattcat agcgtctgca acgtgcagga cgaggtcggg tgtaccatgc aatgccgcct
    22321 aacaaggcac caaccagggc cataaacaga aatccgacca atgctcctaa ccacttcccc
    22381 gcggtagatc ctaaatcact ttcaaatccc gacacggggg catcggggat ccggctcatc
    22441 acccagatgt atcgcgccat tgcccagacg aaatagccac accatgtata gaaagcccac
    22501 aaagccaatt tgccgccagg actgcgagag agtctctctt ccatctttgt cgaaccgtat
    22561 tccattttga gataacccgg aaaattcaga cttagaacta ttatgtgata aaaatcacat
    22621 tttt
//
LOCUS       DAZFJL010000079        21533 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFJL010000079 DAZFJL010000000
VERSION     DAZFJL010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.079"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(82..768)
                     /locus_tag="ACHJ8C_002899"
     CDS             complement(82..768)
                     /locus_tag="ACHJ8C_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFO5552724.1"
                     /translation="MSFMAFVTCLTIAFVAGFVVARKLTLRKVASSVIISKSQPVPSS
                     ATPPAAKASTRSYTPQKTYRDSRWQERDELFQGMVAEGILLEGKEWLITRCEKIYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VGVIELDDPSHERADRIIRDHWLDTIMTLARVPFVRASVTKLEEIPPVWDTRDKIRPL
                     VAAKKSEPLH"
     gene            complement(1331..2119)
                     /gene="phoH"
                     /locus_tag="ACHJ8C_002900"
     CDS             complement(1331..2119)
                     /gene="phoH"
                     /locus_tag="ACHJ8C_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO5552725.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(2747..4018)
                     /gene="efeB"
                     /locus_tag="ACHJ8C_002901"
     CDS             complement(2747..4018)
                     /gene="efeB"
                     /locus_tag="ACHJ8C_002901"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO5552726.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPRMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTLDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNAKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFDRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(4024..5151)
                     /gene="efeO"
                     /locus_tag="ACHJ8C_002902"
     CDS             complement(4024..5151)
                     /gene="efeO"
                     /locus_tag="ACHJ8C_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO5552727.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADIPQVKVTVTDKQCEPM
                     TITVNTGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKEGFENYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5209..6039)
                     /gene="efeU"
                     /locus_tag="ACHJ8C_002903"
     CDS             complement(5209..6039)
                     /gene="efeU"
                     /locus_tag="ACHJ8C_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO5552728.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFVLPPRAGATASRSV
                     "
     gene            complement(6259..7767)
                     /gene="putP"
                     /locus_tag="ACHJ8C_002904"
     CDS             complement(6259..7767)
                     /gene="putP"
                     /locus_tag="ACHJ8C_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO5552729.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPVVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(7926..8135)
                     /locus_tag="ACHJ8C_002905"
     CDS             complement(7926..8135)
                     /locus_tag="ACHJ8C_002905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5552730.1"
                     /translation="MMLQLCATVLNVTCVASLKMNEMLINEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            8190..12152
                     /gene="putA"
                     /locus_tag="ACHJ8C_002906"
     CDS             8190..12152
                     /gene="putA"
                     /locus_tag="ACHJ8C_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO5552731.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQSVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVELALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLQMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPALIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVLFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(12192..12830)
                     /gene="rutR"
                     /locus_tag="ACHJ8C_002907"
     CDS             complement(12192..12830)
                     /gene="rutR"
                     /locus_tag="ACHJ8C_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO5552732.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            13118..14209
                     /gene="rutA"
                     /locus_tag="ACHJ8C_002908"
     CDS             13118..14209
                     /gene="rutA"
                     /locus_tag="ACHJ8C_002908"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO5552733.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTNTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            14209..14901
                     /gene="rutB"
                     /locus_tag="ACHJ8C_002909"
     CDS             14209..14901
                     /gene="rutB"
                     /locus_tag="ACHJ8C_002909"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO5552734.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRNQP
                     QLQGKLLAKGSWDYQLVDELMPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSSTSFARIA"
     gene            14913..15299
                     /gene="rutC"
                     /locus_tag="ACHJ8C_002910"
     CDS             14913..15299
                     /gene="rutC"
                     /locus_tag="ACHJ8C_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO5552735.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            15307..16107
                     /gene="rutD"
                     /locus_tag="ACHJ8C_002911"
     CDS             15307..16107
                     /gene="rutD"
                     /locus_tag="ACHJ8C_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO5552736.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLVQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSRHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNMTDPETFNALLLNGLASLLHHREAAL"
     gene            16117..16707
                     /gene="rutE"
                     /locus_tag="ACHJ8C_002912"
     CDS             16117..16707
                     /gene="rutE"
                     /locus_tag="ACHJ8C_002912"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO5552737.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWREPPVSDETLREIYAQMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            16718..17212
                     /gene="rutF"
                     /locus_tag="ACHJ8C_002913"
     CDS             16718..17212
                     /gene="rutF"
                     /locus_tag="ACHJ8C_002913"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO5552738.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            17233..18561
                     /gene="rutG"
                     /locus_tag="ACHJ8C_002914"
     CDS             17233..18561
                     /gene="rutG"
                     /locus_tag="ACHJ8C_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO5552739.1"
                     /translation="MVMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKNVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQKP"
     gene            complement(18644..18817)
                     /gene="ymdF"
                     /locus_tag="ACHJ8C_002915"
     CDS             complement(18644..18817)
                     /gene="ymdF"
                     /locus_tag="ACHJ8C_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO5552740.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            19190..19786
                     /gene="wrbA"
                     /locus_tag="ACHJ8C_002916"
     CDS             19190..19786
                     /gene="wrbA"
                     /locus_tag="ACHJ8C_002916"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO5552741.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            19807..20034
                     /gene="yccJ"
                     /locus_tag="ACHJ8C_002917"
     CDS             19807..20034
                     /gene="yccJ"
                     /locus_tag="ACHJ8C_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO5552742.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(20072..21309)
                     /gene="agp"
                     /locus_tag="ACHJ8C_002918"
                     /pseudo
     CDS             complement(20072..21309)
                     /gene="agp"
                     /locus_tag="ACHJ8C_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
BASE COUNT         5101 a         5791 c         5498 g         5143 t
ORIGIN      
        1 agagctcgta cgaaagtgcg agcttttttt cgtatattgc atacaccggg gggatgagaa
       61 gccccgaccg gggatacctg gttaatgcaa cggttcactc tttttcgccg cgactaatgg
      121 gcgaattttg tcacgggtat cccagaccgg agggatttcc tccaatttag tgacagaagc
      181 tcgtacaaaa ggcacccgag caagagtcat aatcgtatcc aaccaatggt cacgaataat
      241 gcgatctgcc ctttcatgac tcggatcatc cagctcaata acaccgacta ttttattgtt
      301 cttctttgaa accagcacat aatccattgc catgccatta taaatagcaa agaaacgctt
      361 tctttcttct tcgctaaact cgggttgttc tggaaagttg atcaagcgcc ccagagagac
      421 ctggcaatgg atatggcaat aatgtccaaa ccatttctcc agattatcga aatagatttt
      481 ctcacaacgc gtaattaacc actcttttcc ttccaataaa atgccttcgg caaccattcc
      541 ctgaaataac tcatctcgtt cttgccaacg agaatcacga taggttttct gcggtgtata
      601 gctccttgtc gacgccttag cagcaggggg agttgcagat gaagggactg gttgcgattt
      661 agagatgatc accgatgagg caacttttcg gagtgtcaat tttctggcaa caacaaaacc
      721 ggcgacaaat gcaattgtca aacaagttac aaaagccata aagctcataa ttaattttca
      781 cctatccatg ttgattatat ttcattacca acgttactcg ctgtagcgtg ttacatgcca
      841 acaattcaac gatcaatata tagttagtat catgttttta ttattacaac cacccctatt
      901 ttgccattgc ggcttgcctt ccacaaaaaa taacaacaca aaacattagc caaagaatta
      961 attaagatta acctgacatt tttaatatta tccctaaaaa ataaaatcag gagaatatat
     1021 tcggtgttaa tcagtcagtc aaattttggc ccattgttga ttaaacctca tacgcaaaat
     1081 tggcaccaga cctcctgtac aggttgaatg gctggaaaaa gataggcaaa aaactctccg
     1141 cgctggcggc attacattgg tcgcgtgcta tgacgaaaaa tgcctggtaa aagtatctaa
     1201 accagaatta atttcagctt atcaacacgc tcaaagtcgc cccccagaac gttaacctga
     1261 agtgaaagtc tttgctgtta atgaaataaa acaaagcccg gttcgcccgg gctctgcgcc
     1321 gataatacac ttaactgtat gcatgtagcg tacgttggca aagtgccgaa cgtacgcaat
     1381 cctctttacc gaatcggacg atcccgacca tttcatcttc ttcgaaacgt tccagcgcgt
     1441 cacttaatcc ggagcgcacg ccgcgaggca aatcgcattg cgtgatatca ccgttgacga
     1501 taaccgtcac gttctctccg aggcgggtta aaaacatttt catttgcgcg gcagtcacat
     1561 tctgcgcctc gtcaagaatg acgactgcat tttcaaaggt acgtccacgc atataggcga
     1621 acggcgcaat ttccaccttc cctatttccg gtcgcaggca gtactgcata aaggaagccc
     1681 ctaagcgccg aaccagcacg tcgtagaccg ggcgaaaata gggagcaaac ttttctgcga
     1741 tatcaccagg taagaagcca agatcttcat cagcttgcag aactggacgg gtgacgataa
     1801 tcctgtcgac atccttatgt atcagggcct ctgccgcttt tgctgcgctg atccaggttt
     1861 ttccgcaccc ggcttcgccc gtggcaaata tcagctgctt actctcaata gccttcagat
     1921 agtgcaattg cgcttcattt cgcgcgagga tgggcgaggt atcgcgactg tcgcgggcca
     1981 taccaatggc ttctacgccg ctcatctgca caagcgaggt gaccgattct tcttcacgct
     2041 gcttatggct gcgcgaatcc cgtctcagca cacgttttgc ctcgcgacga gctttgatca
     2101 ctgctttttg tcttcccatg gagagcacct tgagttgttt gtattcatca cacgcgccgt
     2161 tggcagcgcg attatgcgca cgaacatcag agggttggct tccttgtaag ccatagtttg
     2221 cttttggata aaatgccgaa aaacggctac gcgcaccgtt tacggcgtcg gtaacacatg
     2281 aaaagaaagg atgaggttga aaatacaaag tgacgagatg actaccggag gagaaatctc
     2341 cgcgagtggt ggcgcgctga ttatctaaaa catgtccagt acaggacgtt accatccgcg
     2401 atctccatag tgactgacta tcactgccgg gaacttccgc tgctacttaa taagtacaac
     2461 agatctctca ttcattgcaa caatatattc acttatattt aaccataaaa caccatttca
     2521 gtgacattag tttctactgg aaagatgaca aagtgatgac agtgataaaa aaagctgtgt
     2581 gctttcagca ggattcgcag tactggtaaa agcttgccgg atgcggcgtg aacgccttat
     2641 ccggcctacc gaacccgcac cgattgtagg catgataaga cgcgcaagcg tcgcatcagc
     2701 gcacgaacgc ctggtgcggt gtaaacgcct tatccgccta aaaacattaa acccgcaata
     2761 atgcgcttcc gagataatcg ttcgcgtcct tcacacccgg cagcgcaaag aaatagccgc
     2821 caccgatagg tttgacatat tcctccagcg cctcgccatt caaccttttt tgtactgtca
     2881 ggaagccctt ttccagatcg tgttgatagc agacaaacag caaccccata tccagttgcc
     2941 cggagttggt gacgcccaac gaataactgt agccacgacg cagcatcagg ctggactcgc
     3001 tctccgccgt gcggggattc gccagccgga tatggctgtc cagcgcaatc accttccctt
     3061 ccggatcgct agcgtaatcg ggaacatcat gctcatgctg catgcccagc ggcgcaccgg
     3121 tttgcttatc acgatcaaat atcgtctgct gctctttcag cggcgttctg tcccaaaatt
     3181 ccactcgaaa ctgaatcaag cgtactgcct gatagctgcc accgattgtc cacgcaggct
     3241 cctgctgatc tgccgtcacc cacaccactt tttgcatcaa cttcgcattc tggctatcgg
     3301 gattggcggt gccgtcttta aagcccagca aatttatcgg cgtctctttg cctttactac
     3361 gcgctgcgtg gtccgaaata aacccttccc gcttccagcg cacgctgagc aaatccggcg
     3421 tgtgtttgat gatatcgcgc agcgcatgga tcaccgtgtc cagggtgttg gcgcaaattt
     3481 gtagcaacac atcaccatga cataacgccg catccagcga gtcgttaggg aaacgcgtca
     3541 tcttctgcaa cttttttggc atccgtggcg caaggccaaa gcgctcatca aacaatgagt
     3601 gacccaccga taacgtgatg gtgagattat cgggcgcaat gtagccgcca agaatgccgg
     3661 aatcgagtgg tggcaggcgc ggatttggcg tttctggtgc tgctccgccc tgagtcagaa
     3721 aagcaaaacg ctgagtcaac aagcgaaaca accgttcaag atcggcttta tcgctggcaa
     3781 gcacatcaaa cgccaccagc atcattgcag cctgttgtgg tgtcaggatc cctgcctgat
     3841 gctcaccata aaacggctgt ttctcattgc gtgcatccgg tgaaagcgta cccggcgcac
     3901 tttgcgtttt ttgtgcatga gcaaccggac aacttcccgc cagcgccagc gcaccaatcc
     3961 ctttcagtaa acgtcggcgt gacggttcat tcacgccgtt ttcatcttca tactgcataa
     4021 cgcttagtcc agccccagca caccgcgaag ttgcgccaga tcttccgcca gcgcagtaat
     4081 cggtcctttc agtgcattcc ggtcggcatc ggtcagtttg tcgtaatttt caaaaccctc
     4141 tttagtgcgg tatttcgcca gaatagtatc gacctttttg aagttggcat cgacttttgc
     4201 cagcagttcc ggattcgctt tttgtagttg tggacgcagc agatcgacaa ttttctgcga
     4261 gccttcaacg ttagcctgga aatcccacag atcggtgtgg ctgtagcgat cttcttcacc
     4321 gctgattttg ctggccgcca cttcctcaat cagtccggct gcaccgccga ccacttttga
     4381 aggagggaaa gccagttcac tgatgcgttt ttgcaaatcg accacatcgg tataaagctg
     4441 gtcagcgtac ttatccattc ctttggtggt gttatcgcca aacaatgctt tttccagacg
     4501 gtggaaaccg gtgaatttcg gatcagcggc tttttgctcg taatcatctt cacgggcgtc
     4561 aatgctgcca tccagatcgg agaacagttc agcaatcggt tcgatgcgct catagtgctg
     4621 gcgcgtcggc gcatacagtg ctttcgcttt ttcgatatcg cctgctttaa tagcgtcggt
     4681 aaaggctttg gtgtcggtca ccagttgcgt ggtttccgcc atgacatacg ctttatatgc
     4741 agtaattgca ccaccaagac ttaacagcgc atcactttgc gccgcatccg ccgttgcttc
     4801 acctttgacg atcaacttcc ctttcgggtt agtcagcaga ccgcaggtca tatcgtattc
     4861 gccaggctgt aaattcgccg tcattttctg gctaaaacca ggggcgatat tttcccgctc
     4921 ttccaccacc atcacgcctt tgaggatctc ccactccagc gccttctggc tgtggttctg
     4981 aataatgaac tgtgttttcc cggtgttaac cgtaatggtc atcggctcgc actgcttatc
     5041 tgtcacagtg actttgactt gcggaatatc ggcggcgtta gccataaaag cagaagagaa
     5101 cagcgcagcc acgctcaact gcaatgcgtt acggcggaag ttaatggtca tgacgagact
     5161 atccctttaa agtatgttgt aactaagcaa gagtttgcgt cgtatttgct acacggagcg
     5221 ggacgcagtc gcccctgcgc gtggtggcag aacaaatgcc accagcgccg ggatgagata
     5281 aataaaccag acggcgactt cgctgacgct cggtgcttcc tgatagccaa aaatcccttc
     5341 catcagtgtg ccaaacagcg agtgagttga gagcaccgcg ctcatatcga aggcgatttc
     5401 ctgaaagtgg ttccacaatc cggcttcatg aaatgcgcga atggcaccag ctgccagccc
     5461 tgcggcgacg aagagaataa acagactggt ccatttaaaa aatgcaccaa gattgaggcg
     5521 aataccgccc cagtagagca ggaagccaag caccacggct gtagcaagac caagcattgc
     5581 acccagcggc ggccagatcc cgacatcttg ttgaaatgcc gccagcagga aaaagaccga
     5641 ctccagccct tcccttgcaa cggcaaaaaa gaccatcatc accagcgccc agccatgatg
     5701 atttccacgc tgcaatgcgc tatcgaccgc ctgctccagt tgtactttga cgttgcgcga
     5761 cactttgcgc atccagaaaa ccatccaggt aaggatcacc acggcgatca ccgccacaat
     5821 gccttcaaac agttcctgtt ctttttgcgg aaattcgccg gtggtttcgt taatgaagat
     5881 acccaagccc aggcacaacg cagcggcaag caacacgcca atccacatta caccaatcca
     5941 tcggcctcgc tgggtacgct taagatagct ggcaatcaaa ctgacaatca gcgcggcttc
     6001 aagtccttcg cgcaacataa tgagaaacgg aacaaacatg ccacccaccc ttaaacgttt
     6061 ttctgagtca atctatgcaa agaaaagtaa atcgcagtga tagtgattat cattacgatg
     6121 aagaaaaata caagtggaat gtagtgataa tgatgagaag atgtaaaaat attttgagaa
     6181 aaaacgcaga gggcaatcac tgattgccct ctgcctgctt cctgttatga cgtgaaattt
     6241 ccggtaaaac gccttccctt acccttcctg caaccgtgac ggcggagccg aatgatagtg
     6301 cgcatcggcc tcggcaaagc gtttttgcat cgccgctgac ggcgctttac ccagcaaact
     6361 aaacactaca atcccgatac tgccgaagat aaagcccgga ataatttcgt acagacccag
     6421 ccagccgaac tgtttccaga cgataaccgt cagcgcaccg atgatcatcc ccgccagcgc
     6481 accgttgcgc gtcatgcgtg accacatcac cgagaacagc accaccggac caaacgctgc
     6541 gccaaagcct gcccacgcgt agctcactaa gcccagcacg cggttttccg ggtttgccgc
     6601 cagcgcaatc gccaccagcg ccaccaccag caccatcact cgccctaccc acaccagctc
     6661 tttctggctg gcctgtttac gcagaaacgc tttgtataaa tcttcggtaa tcgcactgga
     6721 acataccagc agctggcaac ttaacgttga cattaccgcc gccagaatcg ccgacagcaa
     6781 aatcccggca atccacgggt taaacagaat ttgtgccagt tcaataaaca cgcgctcggc
     6841 gttctggttt accgcacctg ctaccaccgg gtgctcgtta aagtaagcga taccaaagaa
     6901 gccgacagcc accgcccctg ccaggcagag gatcatccag gtcatactga tacgacgcgc
     6961 atggacaatg ctgtggtgag aatctgccgc cataaaacgc gccaggatgt gcggctgccc
     7021 gaagtaaccc agcccccaac ccatcagcga gataatggcg acaaaattca accctttgag
     7081 catatccacg ttttcaatgc tcttttgttt gattacttcc agcgagtcac caaagccacc
     7141 gacactgata atgacgataa ctggcgtcag gatcagggca aaaatcatca gactggcctg
     7201 tacagtgtca gtccagctca ccgcgaggaa accgccaata aaggtgtaaa ggatcgtcgc
     7261 cgcagccccc gcccacagag ccgtttcgta gctcatgcca aaggtacttt caaacagacg
     7321 cgcgcctgcc acaatgcctg aagcgcaata aatggtgaag aacagcaaaa taaccagcgc
     7381 ggagataatg cgcaaaatgc ggcttttatc ttcaaagcgc ccggtgaaat aatccggcaa
     7441 tgttaaggcg ttattgttgt attcggtatg cacacgcaac cgcccggcca ccagtttcca
     7501 gttgatccac gcgcctaatg tcagaccaat ggcgatccag ctttcggaaa tcccggaaag
     7561 aaaaacagcg cctggcaacc ccattaacag ccagccgctc atatccgacg cacccgccga
     7621 taatgccgtc acgaatggcc caagactacg accgcccaga atatagtcgt cgaagttttt
     7681 cgttgatcgc caggcaataa acccaatcaa tatcatgcca aagatataga cacaaaatgt
     7741 caccaacatc ggtgtgctaa tagccatcta aagtctccaa aaaattatta tcggcaatgt
     7801 cgaaacttgc cgttatatct gccaccggaa cggggtaaca gagtttatgt tttaccaggg
     7861 cgaccgtatc ctgccggaag cgctggttat tcacaatcga tttaacacac catttacatt
     7921 aaattttagt gctcagcgac actatttttc atcaggttgc actctctcac attttttgcg
     7981 gttgcacctt tcaaaaatgt taactgccgc agagaaaaag tctgagttat ttttttaatc
     8041 cctgtcatat cgatttcgtt tattaacatt tcattcattt ttaagcttgc tacgcaggtc
     8101 acatttaaca cggttgcaca aagttgcaac atcatggata tttcacgata acgttaagtt
     8161 gcacctttca gaacaacagg agtaatggca tgggaaccac caccatgggg gttaagctgg
     8221 acgacgcgac gcgtgagcgt attaagtctg ccgcgacacg tatcgatcgc acaccacact
     8281 ggttaattaa acaggcgatt ttttcttatc ttgaacaact ggaaaacagc gatactctcc
     8341 cggagctacc agcgctgctt tctggcgcgg ccaatgagag cgatgaagca ccgactccgg
     8401 cagaggaacc acaccagcct ttcctcgact ttgccgagca aatattgccc cagtcggttt
     8461 cccgcgccgc catcaccgcc gcctatcgcc gcccggaaac cgaagcggtt tctatgctgc
     8521 tggaacaagc tcgcctgccg cagccagtag ctgaacaggc gcacaaactg gcgtatcaac
     8581 tggccgataa actgcgtaat caaaaaaacg ccagtggtcg cgcaggtatg gttcaggggt
     8641 tattgcagga gttttcgttg tcatcgcagg aaggcgtggc gctgatgtgt ctggcggaag
     8701 cgttgttgcg tattcccgac aaagccaccc gtgacgcgtt aattcgcgac aaaatcagta
     8761 acggtaactg gcagtcacac attggtcgta gcccgtccct gtttgttaat gccgctacct
     8821 gggggctgct gtttaccggc aaactggttt ccacccataa cgaagccagc ctctcccgct
     8881 cgctgaaccg cattatcggt aagagcggtg aaccgctgat ccgcaaaggt gtggatatgg
     8941 cgatgcgcct gatgggcgag cagttcgtca cgggcgaaac catcgcggaa gcgttagcca
     9001 atgcccgcaa actggaagag aaaggtttcc gttactctta cgatatgctg ggcgaagccg
     9061 cactgactgc cgcagatgca caagcgtata tggtttccta tcagcaggcg attcacgcca
     9121 ttggtaaagc gtctaacggt cgtggtatct atgaaggtcc gggcatttca atcaagctgt
     9181 cggcgctgca tccgcgttac agccgcgccc agtatgaccg ggtaatggaa gagctttacc
     9241 cgcgtctgaa atcactcacc ctgctggcgc gtcagtacga tattggtatt aacatcgatg
     9301 ccgaagaggc cgatcgtctg gagatctccc tcgatctgct ggaaaaactc tgtttcgagc
     9361 ctgaactggc aggctggaac ggcatcggtt ttgttattca ggcttatcaa aaacgctgcc
     9421 cgttggtgat cgattacctg attgatctcg ccacccgcag ccgtcgccgt ctgatgattc
     9481 gcctggtgaa aggcgcgtac tgggatagtg aaatcaagcg tgcacagatg gacggccttg
     9541 aaggttatcc ggtttatacc cgcaaggtgt ataccgacgt ttcttatctt gcctgtgcga
     9601 aaaaactgct ggcggtgcca aatctaatct acccgcagtt cgcgacgcac aacgcccata
     9661 cgctggcggc aatttatcaa ctggcgggtc aaaactacta cccgggtcag tacgagttcc
     9721 agtgcctgca tggtatgggc gagccactgt atgagcaggt caccgggaaa gttgccgacg
     9781 gcaaacttaa ccgtccgtgt cgtatttatg ctccggtcgg tacacatgaa acgctgctgg
     9841 cgtatctggt gcgccgcctg ctggaaaacg gtgctaacac ctcgtttgtt aaccgtattg
     9901 ccgacacctc tttgccactg gatgaactgg tcgccgatcc agtcactgct gtagaaaaac
     9961 tggcgcaaca ggaagggcaa actggattac cgcatccgaa aattcccctg ccgcgcgatc
    10021 tttacggtca cgggcgcgac aactcggcag ggctggatct cgctaacgaa caccgtctgg
    10081 cctcgctctc ctctgccctg ctcaatagtg cactgcaaaa atggcaggcc ttgccaatgc
    10141 tggaacaatc ggtagcggct ggtgagatgt cgcccgttat taaccctgcg gaaccgaaag
    10201 atattgtggg ctatgtgcgt gaagccacgc cgcgtgaagt agaactggcg ctggaaagtg
    10261 cggttaataa cgcgccaatc tggtttgcca cgcctccggc tgaacgcgcg gcgattttgc
    10321 atcgcgctgc cgtgctaatg gaaagccaga tgcagcaact gattggtatt ctggtgcgtg
    10381 aagccggaaa aaccttcagt aacgccattg ccgaagtgcg cgaagcggtc gattttctcc
    10441 actactatgc cggacaggtg cgggatgatt tcgctaacga aacccaccgt ccattagggc
    10501 ctgtggtatg tatcagtccg tggaacttcc cgctggcgat tttcaccgga cagatcgctg
    10561 ccgcactggc ggcaggtaac agcgtgctgg caaaaccggc agaacaaacg ccgctgattg
    10621 ccgcgcaagg gatcgccatt ttgctggaag cgggtgtacc gccaggcgtg gtgcaattgc
    10681 tgcccggtcg gggtgaaacc gtgggcgcgc agctgacggg tgatgatcgc gtgcgcgggg
    10741 tgatgtttac cggttcaacc gaagtcgcta cgttactgca gcgcaatatc gccagccgcc
    10801 tggacgctca gggtcgccct attccgctca tcgctgaaac cggcggcatg aacgcgatga
    10861 ttgtcgattc ttcagcactg accgaacaag tcgtaataga tgtgctggcc tcggcgttcg
    10921 acagtgcggg tcagcgttgt tcggcactgc gcgtgctgtg cctgcaagat gagattgccg
    10981 accacacgtt gcaaatgctg cgcggcgcaa tggccgaatg ccggatgggt aatccgggtc
    11041 gcctgaccac cgatatcggt ccggtgattg atagcgaagc gaaagccaat atcgagcgtc
    11101 atattcagac catgcgtagc aaaggccgtc cggtgttcca ggcggtgcgg gaaaacagtg
    11161 aagatgcccg tgaatggcaa agcggaacct ttgtcgcccc gacgctgatc gaactggatg
    11221 actttgccga attgcaaaaa gaggtctttg gtccggtgct gcatgtggtg cgttacaacc
    11281 gtaaccagct gccggcgctg atcgagcaga ttaacgcttc cggctatggt ctgacgcttg
    11341 gcgtccatac acgtattgat gaaaccatcg cccaggtcac tggctcggcg catgttggta
    11401 acctgtacgt taaccgtaat atggtgggcg cagtggttgg ggtgcaaccg ttcggcggcg
    11461 aagggttgtc tggcaccggg ccgaaagcag gcggtccact ctatctctac cgtctgctgg
    11521 cgaatcgccc ggaaagtgcg ctggcggtga cgctcgcgcg tcaggatgca gagtatccgg
    11581 tcgatgcgca gttgaaagcc gcattgactc agccgctaaa tgcactgcgg gaatgggcgg
    11641 caaaccgtcc agaattgcag gcattatgta cgcaatatgg cgagctggcg caggcaggaa
    11701 cacaacgatt gttgccaggg ccgacgggtg aacgcaacac ctggacgctg ctgccgcgcg
    11761 agcgcgtgtt gtgtattgcg gatgatgaac aggatgcgct gactcagctc gccgccgtgc
    11821 tggcggtggg cagccaggta ctgtggccgg atgacgcgct gcatcgtcag ttagtgaagg
    11881 cattgccatc ggcagtcagc gaacgtattc aactggcgaa agcggaaaat ataaccgctc
    11941 aaccgtttga tgcggtgctc ttccacggtg attcggatca gcttcgcgca ttgtgtgaag
    12001 ccgttgccgc gcgggatggt gcaattgttt cggtgcaggg ttttgcccgt ggcgaaagca
    12061 atatccttct ggaacggctg tatatcgagc gttcgctgag tgtgaatacc gctgccgctg
    12121 gcggtaacgc tagtttaatg acgatcggtt aaacaggtag ccggaggatg ttacaacctc
    12181 ctccggcatc attagcgtgg tcgaatcccc tcaataataa tccgctgcac gttttcaacc
    12241 gtttgattga aaaatacctc atcgcgcaac gtcgcgcctg tcaccgcctc cacctgaggg
    12301 gcgaaatcgg cgtaatgttg agtggaagcc caaatcataa aaatcaaatg ctgcgggtca
    12361 atcggcgcga gtttgccgct tttaacccaa ccggcaatca gcgccgattt ctcatcaatt
    12421 aatgccttca aatcgcccgt cagttcatcc attaacagcg gtgcacctgc cagcatctcc
    12481 atacagaaca ggcgcgaagc ctgcggataa tcgcgggaaa cttccagctt cagacggatg
    12541 tactctttga tcgccgccag cggggcgaaa tcttctcgaa acgcttttaa cggtgccagc
    12601 cagatatcga gaatctgccg cagtacggct atatacagcg cctcttttga cggaaagtaa
    12661 tacagcagat tggtttttga aacacccgcc aactccgcga tctgctccag ccttgtgccg
    12721 tgaaaaccga attgtgaaaa agtgtccagt gctgcgctaa gaatcgcttt tttcttcgcg
    12781 cttactgcgc gcgaacgctt acccgttgtt ttcactgcgc cttgcgtcat gcgctctccc
    12841 ctcttgttga ttggcaacag gatagcaaaa cccccgcccg gcctcgacct tctgcactct
    12901 catcgcgcac gaatgcatgt tttatgtgca actgttttga ccgtttagtc cactttttac
    12961 cagacatctt agataacgca atattaacac attaataaca ttcacttttt aaaaactggc
    13021 atccgctttg caaacaagcc attacgcggc tgaaaaggat atgcaggatg cagcgccccg
    13081 tctgactttc actttacgag acgaagagag gttgatgatg aaaattggcg tattcgtacc
    13141 tattggcaac aacggctggc tcatttcgac ccacgcgccg cagtacatgc cgacatttga
    13201 actgaataaa gccattgtgc aaaaagcgga gcactaccat ttcgatttcg ccctgtcgat
    13261 gatcaaactg cgtggctttg gcggcaaaac tgagttctgg gatcacaacc ttgagtcgtt
    13321 caccttgatg gcggggctgg cagccgtcac ctcacgcatt cagatatacg cgacagctgc
    13381 caccttaacg ttacctccgg caatcgtcgc ccgtatggcc gcaaccatcg actccatctc
    13441 tggcgggcgt tttggcgtca acctcgtgac tggctggcag aagcccgagt atgagcagat
    13501 ggggatctgg cctggcgatg actatttctc ccgtcgttac gactatctca ccgaatatgt
    13561 tcaggtgctg cgcgacctgt ggggctcggg gaaaagcgat tttaaaggcg attttttcac
    13621 tatggatgat tgtcgcgtca gtccgcaacc gagtgtcccc atgaaagtga tctgcgccgg
    13681 gcaaagcgac gctggcatgg cgttctccgc ccggtatgcc gattttaact tctgtttcgg
    13741 caaaggcgta aatacaccca cggctttcgc cccgaccgct gcgcggatga aacaggccgc
    13801 agagcaaacc ggacgcgacg ttggctctta tgtgttgttt atggtgattg ccgacgaaac
    13861 cgacgatgcc gctcgtgcaa aatgggaaca ctacaaagcg ggcgcggatg aagaggcgtt
    13921 aagctggcta accgaacaaa gtcagaaaga tacccgctcc ggtacaaaca ccaacgtccg
    13981 tcagatggcc gatcccactt cggcggtaaa catcaatatg gggacgttag tcggttctta
    14041 cgccagtgtc gcgcgcatgt tagatgaagt cgcaagcgtg cctggtgccg aaggcgtgct
    14101 gttgaccttc gatgattttc tgtcgggaat cgaaaccttc ggcgagcgca ttcaaccact
    14161 gatgcagtgc cgcgcccatc tccctgcgct gactcaggag gtggcatgat gacgacctta
    14221 accgctcgac cggaagccat taccttcgat ccgcagcaaa gtgcgctgat cgtggtggat
    14281 atgcaaaacg cttatgccac gccaggcggc tacttagatc tcgccgggtt tgatgtctca
    14341 accactcgcc cggtcattgc caacattcaa accgccgtga ccgcagcacg agcggcaggg
    14401 atgctgatca tctggtttca aaatggctgg gatgaacagt atgtcgaggc tggcggcccc
    14461 ggctcaccaa attttcataa atcgaacgcc ctgaaaacca tgcgtaatca gccgcagctg
    14521 caggggaaat tgctggcgaa aggctcctgg gattatcaac tggtggatga actgatgccg
    14581 cagcctggcg atattgtgct gccgaagccg cgctacagcg gtttcttcaa tacgccgctg
    14641 gacagcattt tgcgcagccg tggaatacgc catctggttt tcaccggtat cgctaccaac
    14701 gtctgcgtcg aatcgacgct acgcgacggc ttttttctgg agtatttcgg cgtggtgctg
    14761 gaagacgcaa cacaccaggc ggggccggaa tttgcacaga aagccgcgtt gttcaatatc
    14821 gaaacctttt ttggctgggt cagcgacgtc gaaacgttct gcgacgcgct ttcttccacg
    14881 tcctttgctc gtatcgctta aggagtttaa cgatgccaaa atccgtaatt attcctgctg
    14941 gcagcagcgc accgctggcc cccttcgttc ccggcacgct ggctgatggc gtggtgtatg
    15001 tctccggtac gctggctttt gatcaacata acaacgtgct gtttgccgat gacccaaagg
    15061 cgcaaacccg ccacgttctg gaaaccatcc gcaaggtcat cgagacggcg ggtggcacaa
    15121 tggcggatgt gactttcaac agcatcttta ttaccgactg gaaaaattac gccgcgatta
    15181 acgaaatcta cgccgagttt ttcccgggtg acaaaccggc gcgattctgc attcagtgcg
    15241 gactggtaaa acctgacgcg ctggtggaaa tcgccacaat tgcgcatatc gccaagtgag
    15301 gccgcgatga aactttcact ctcacctccc ccttatgctg atgcgcccgt agtggtgttg
    15361 atttcgggtc ttggcggtag cggcagttac tggttaccgc aactggcggt gctggttcag
    15421 gagtatcagg tagtctgtta cgaccagcgc ggcaccggca ataatcccga cacgctggca
    15481 gaagattaca gtatcgccca gatggcagcg gaactgcatc aggcgctggt agccgcaggg
    15541 attgagcgtt acgcggtggt cggccatgcg ctcggtgcgc tggtgggaat gcagctggcg
    15601 ctggattatc ccgcgtcggt aactgtgctg gtcagcgtta acggctggct acgaataaac
    15661 gcccatacgc gccgctgttt tcaggttcgc gaacagttac tacatagcgg cggcgcgcag
    15721 gcatgggtgg aagcgcagcc gttgttcctc tatcccgccg actggatggc ggcccgcgca
    15781 cctcgccttg aggcagaaga cgcgctggca ctggcgcatt ttcagggcaa aaataattta
    15841 ctgcgtcgac ttaacgccct caaacgcgct gactttagtc gccatgcgga tcgtatccgc
    15901 tgcccggtgc aaatcatctg cgccagtgat gatctgctgg tgccatcagc atgttccagt
    15961 gaacttcatg ccgctctgcc cgatagccag aaaatggtga tgcgctatgg cggacacgcc
    16021 tgcaacatga ccgatcccga aacgtttaat gctctgttac tcaacgggct tgccagcctg
    16081 ttacatcacc gtgaagccgc cctgtaagga attgctatga acgaagccgt tagcccaggt
    16141 gcgcttagca ccctgttcac cgatgcccgc actcacaacg gctggcggga gccccccgtc
    16201 agcgatgaga cgttacggga gatttatgcc cagatgaaat gggggccgac atcagctaac
    16261 tgttctccgg cacggatcgt gtttatccgc actgcagaag gaaaagaacg tctgcgcccg
    16321 gcgctttcca gcggcaatct gcaaaaaacc ctgaccgcgc ccgtcaccgc tatcgtcgcc
    16381 tgggacagtg aattttatga acggttacca cagctgtttc ctcacggcga tgcccgcagt
    16441 tggtttacct ccagcccaca acttgccgaa gaaacagcgt ttcgcaacag ttccatgcag
    16501 gcggcctatc tgatcgtcgc ctgccgtgcg ctggggctgg ataccggccc gatgtcgggc
    16561 tttgaccgtc aatacgttga cgacgccttt tttgcgggca gcacgctgaa gagcaatctg
    16621 ctgattaata tcggctatgg cgattccagc aaactttttg cgcgcctgcc acgtctgtcc
    16681 tttgaagaag cctgcgggct gttgtaagga gcaaacaatg aacattgtcg atcaacaaac
    16741 ctttcgcgat gcgatgtcct gtatgggcgc ggcggtcaat atcatcacta ctgacggtcc
    16801 agccgggcgc gccgggttca ccgccagcgc cgtctgcagc gtgaccgaca cgccgcccac
    16861 attactggtg tgcctgaatc gtggggcgtc cgtctggccg gtattcaatg aaaatcgaac
    16921 gctgtgtgta aatacactta gcgccgggca ggagccgctt tcaaaccttt ttggcggcaa
    16981 aacgcccatg gaacaccgct ttgccgccgc ccgctggcag actggtgtga ccggatgccc
    17041 gcaactggaa gaggcactgg tttcgtttga ctgccgtatc agccaggtgg tgagcgtcgg
    17101 cacccacgac attttgtttt gcgccatcga agcgattcat cgtcacgcca caccctacgg
    17161 gctggtgtgg ttcgatcgca gttatcacgc gctgatgcgc cctgcttgtt aacctcctta
    17221 aggagacagc tgatggtaat gttcggtttt cctcactggc agttaaaatc gacctctaca
    17281 gagagcggcg tggtcgcgcc ggatgaacga ttgccgtttg ctcaaacggc agtaatgggc
    17341 gttcaacacg cggtggcgat gtttggcgcg acggtgttaa tgccaattct gatggggctg
    17401 gatcccaatc tttccatttt aatgtcgggg atcggcacgt tactgttttt ctttattacc
    17461 ggcgggcgcg tgcccagtta tctcggttcc agcgcggctt ttgtcggcgt ggtgattgcc
    17521 gcgacgggct ttaatggtca gggaattaat ccgaatatca gcatcgcgct gggcgggatt
    17581 atcgcctgtg ggctggttta taccgtcatt ggcctggtgg tgatgaaaat cggtacgcgc
    17641 tggattgaac gactaatgcc gccggttgtg acgggggcgg tagtgatggc gattggcctg
    17701 aacttagcgc ctatcgcagt gaaaaatgta tcggcttcgg cctttgatag ctggatggcg
    17761 gtaatgacgg tgctgtgtat tgggctggtg gccgtattta cccgtggcat gatccagcga
    17821 ttattgattc tcgtaggatt aattgtcgcc tgtctgctgt atggcgtgat gaccaacgtt
    17881 ttagggctgg gaaaagccgt cgattttact ctcgtcagcc atgctgcctg gttcggtctg
    17941 ccacattttt ccactcctgc ttttaatggt caggcaatga tgctgattgc gcccgtggcg
    18001 gtgattctgg tggcggaaaa cttaggtcat ctcaaggcgg tcgccgggat gaccggacgc
    18061 aatatggacc cgtatatggg gcgggcattc gtaggcgatg ggctggcgac gatgctttct
    18121 ggctctgtcg gcggtagcgg cgtaaccacc tacgccgaga atattggtgt gatggccgtg
    18181 acaaaagtct actcgacgct ggtgtttgtt gccgctgccg tcatcgccat gctgttgggc
    18241 ttttcgccga aatttggcgc gctgatccat actattccgg ctgcggtgat tggtggtgca
    18301 tcaattgttg tgttcgggct gattgccgtc gcaggcgcac ggatctgggt acaaaaccgt
    18361 gtggatttaa gccagaacgg taatttgatt atggtcgcag taaccttagt gctgggtgcg
    18421 ggtgattttg ctctcacgct gggcggtttt acgttgggag ggatcggtac agccaccttt
    18481 ggcgcgattt tactcaatgc gttgttaagc agaaggttgg ttgacgttcc accgccagaa
    18541 gttgttcatc agaagccata aacagaaaac cgccgggatt accggcggtg atggcttttt
    18601 gacgagctac agacaaaaat gcatgcgtca ttgattagcc cggctagttg tcgcttttac
    18661 cgtgactgct cttgccacct tttttacctg cttcagatgc gcgctgcggg tcatttttga
    18721 aattaccccc gctgtgctgt ccaccttttt tacctgcttc tgatgctctt tcgcggtctt
    18781 ctgcaaaatt gccggaaccg cctcgatggt ttgccattac tgacctccgt ctgggtatag
    18841 acatcatatt gcatagaatt aacgcggcgt atggcggctg ccatattttc gctacgtggg
    18901 actaagctta gtgaactgac gcgggcagac ccgcgtttgg taatatcctg caacagcttc
    18961 ttagcgagaa tagatatcat ctccaataat tatccataag ccgctcttat gattaaaaaa
    19021 acgcgatgag ataaaaatgt gtcattttgc gacaaaatta cgtgcttgtg aaagttgtta
    19081 taaatcaaat aagtggttgt gaaatttgca ctctgaaaag gacgtcttat ctttaaataa
    19141 gtggtagcga atcgctacgg aatagagata acacgaggag tggttagaaa tggctaaagt
    19201 tctggtgctt tattattcca tgtacggaca tattgaaacg atggctcgcg cagttgctga
    19261 gggtgcaagc aaagtggatg gcgcagaagt tgtcgttaag cgtgtaccgg aaaccatgcc
    19321 gccgcaatta tttgaaaaag caggcggtaa aacgcaaact gcaccggttg caaccccgca
    19381 agaactggcc gattacgacg ccattatttt tggtacacct acccgctttg gtaacatgtc
    19441 cggtcaaatg cgtaccttcc tcgaccagac gggcggcctg tgggcttccg gcgcactata
    19501 cggaaaactg gcgagcgtct ttagttccac cggtactggc ggcggtcagg aacaaaccat
    19561 cacatccacc tggacgaccc ttgcgcatca cggcatggtg attgtcccca ttggctacgc
    19621 agcgcaggaa ttatttgacg tttcacaggt tcgcggcggt acgccgtacg gcgcaaccac
    19681 catcgcaggc ggtgacggtt cacgccagcc cagccaggaa gaactgtcta ttgctcgtta
    19741 tcaaggggaa tatgtcgcag gtctggcagt taaacttaac ggctaatctt caacaggagg
    19801 atacgcatgc caactcaaga agcgaaagct catcacgtcg gtgaatgggc atctctgcgc
    19861 aatacgtcgc cggaaatagc cgaggccatt ttcgaagtcg ccgggtacga cgaaaaaatg
    19921 gcggaaaaga tttgggaaga aggtagcgat gaagtgttag tcaaagcctt tgctaagacc
    19981 gataaagatt cgcttttttg gggcgaacag accatcgaac gtaaaaacgt ttaaccagcg
    20041 actcccccgc ttcacgcggg ggagttttct gttatttcac cgcttcattc aatacgcgat
    20101 caaacttatc cattgggcaa aaaccgttgg catctatcgg gcaaccgctt aattccagcg
    20161 tcacgcgctg cgctggtgcc tgcagggtta acgcatcggc attacgtaac tgttccgcac
    20221 tctggtacac atattcaatt ttcatcaggt cgcgattggc tttgctgtca tgccaacgct
    20281 ggaaaacgat tttgccgcca atcggcgttc gttctggtca tgcaactgat acggtttgaa
    20341 atccagcgcc gttaacagag aggcaatgtt ggagtcgtgc ccaactaata ccgtgatttt
    20401 cggagcactg gtgcgatcgg tgaccagggc tttgtcgata taactgacca gcggtttcgc
    20461 aacattgcgc gccacttccg gtgaggtaaa caggctgtcc tggtaaccgt ttttcagctt
    20521 cgacaacacc ttccactgct ggtcagattt gatttctccc caggccacct gatccatcgg
    20581 aaaaccttcg tagtattgca aagtaaacgc atctaccagc gagttgccga ctttcagcgg
    20641 cccggaaacg cccggttctt gttgatactt cgcgctaaag gtatttttgc catctacaag
    20701 cgaacattgt tgcttctctt tacacgcagg agaatcttta tagttaacga tttgttccag
    20761 tagcttgtag ctgtcggtaa gctggagttt gctgagctct ttctccattg ccgccaccgc
    20821 ctgttcactg aatgcggcgg aatcatcggt gatcactggg ttaaaggtgg ggtccatagt
    20881 gcccattttt tcctggtgat gaacaggaat atcacaccca gggaatgcgc cagtaataaa
    20941 gaactgcgcg gtcgcgacgg tgcgttgcag actattggca taggcataaa cggtgtccgg
    21001 tggcgggcat tcccccgatt tcaccatccc ctgttctgcc agccattcac gcatataatg
    21061 gcccatatac acttcgagca cgcccccttt agtggtgagc tgcccaccgg ggacgtccca
    21121 ttcaggccat ttattcggcg tcgactgctc cagcacactg ccattgttcg ccagcggcgc
    21181 acgtaagtta tggcggctca tcatgagcac ttgctgtagc tgatagcctt ccggtacggt
    21241 ttgtgcctga gcatttgaag cgagtaaaac tatccctgcc acagttgcgg cgattagcgt
    21301 tttgttcatt cctcacacct cttttgttat caataattaa agagtgtgac agaaatatgt
    21361 catttttccg ggaacaggtt cgcaaaatgg ctcgttgaac gcctgttttt ctatattccc
    21421 ctttaaaaac aggcaagttt tgacgcaaac tatgcctgat tagccccggc ctcgtttacg
    21481 ccgctaatga ttacatcagt agctataaag aaaatcagcg gcgttaccat tac
//
LOCUS       DAZFJL010000080        21370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFJL010000080 DAZFJL010000000
VERSION     DAZFJL010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.080"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(87..791)
                     /locus_tag="ACHJ8C_002919"
     CDS             complement(87..791)
                     /locus_tag="ACHJ8C_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5552743.1"
                     /translation="MTIVEINHLQVTFAEKTAVSAASFSVNAGETFSLIGESGCGKST
                     ILRVIAGLQRDWHGQVSLFGHTIKPGMRFQGDLRRNVQMVFQDPYASLHPNHTLWRTL
                     AEPLKIRGEREIEKRVQTALEQVGLPFDTARRYPHQLSGGQRQRVVIARALLLRPQLL
                     LLDEPTSALDMSVQAEILNLLNQLKLEHQMTYLLVSHDADVIAHMSDRAALMSEGKIQ
                     RFFDRDAMEKGEHRMD"
     gene            complement(778..1614)
                     /locus_tag="ACHJ8C_002920"
     CDS             complement(778..1614)
                     /locus_tag="ACHJ8C_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5552744.1"
                     /translation="MTDSRLSINSLCVDYPESRVVNNVSFTLGNECLALVGESGSGKS
                     MTARALMGLVRKPGVVTADTLNILGRDALTLNARGWQQLRGNDIAMVLQDPRYALNPV
                     KTVKAQLEEALTLHQRLNRREKEEKINTAIQAVGLNTRVLQSYPRELSGGMGQRVMIA
                     IALINDPRVLIADEPTSALDVRLRNQILELLVTQCEQRQMAMLLISHDLPLVAQFCHR
                     VLVMYQGNKVDEMHAAALPTATHPYTRTLWTCRPNAQTYGQMLPTLDRTAMTPEKYHD
                     DC"
     gene            complement(1607..2440)
                     /locus_tag="ACHJ8C_002921"
     CDS             complement(1607..2440)
                     /locus_tag="ACHJ8C_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5552745.1"
                     /translation="MPMFLFLRRLRHSPAAFCGLIIILLLMLIALFAPWLAPHDPNWQ
                     DAAARLQAPSRQHWLGTDSYGRDLLSRLIYGSRPALGLVALVTIITLPVGLLIGILAG
                     YYGGWLERVLMRFTDVVMSMPRLILAFAFVAMLGPGLVNGALALALTTWPAYARQARS
                     EIQRLRHSDYLAAAEMMDIRGRRLLAGHILPLCLPSAIVRLALDLAGIILAAAGLGFL
                     GLGARPPMAEWGAMIADGMQVIFDQWWIAAAPGAIILIASLAFNLLGDGLRDVLEPQH
                     D"
     gene            complement(2440..3489)
                     /locus_tag="ACHJ8C_002922"
     CDS             complement(2440..3489)
                     /locus_tag="ACHJ8C_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5552746.1"
                     /translation="MSVVFSTRARWRGKRPLLALLQGLFTVALTLLGLLLITFALSAL
                     SPVDRVLQIVGDHASQSTYDQVRHQLGLDQPLAVQFWHYLMNLAHGDLGIASSTGQPV
                     LQDLLSAFPATIELATLALIVGAVVGVIAGVLCARYVGSPLDFTVRTLTLLGNSVPVF
                     WLGLLMLALFYARLQWSAGPGRLDDIYQFTIEPRSGFALIDTWLSGDREAFTNAINHL
                     ILPVLLLAYFSLASITRLTRSACLGEMNKEYILLARAKGASEMTILLRHVLPNIRGTL
                     LTVIALAYTSMLEGAVLTETVFSWPGIGRYLTTALFAGDTTAIMGGTLLIGVCFVLIN
                     NLTDLLVRLTDPRLR"
     gene            complement(3589..5157)
                     /locus_tag="ACHJ8C_002923"
     CDS             complement(3589..5157)
                     /locus_tag="ACHJ8C_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5552747.1"
                     /translation="MIKKLLPLLVLSTISATTVAATPPNTLVVAQGLDDIVSLDPAEA
                     NELSSIQTVPSLYQRVVQPDRNDPTKITPILAESWQADPAAKTLTIKLKSDAKFASGN
                     PVRPEDVIYSYVRAVTLNKSGAFILNVLGWQPENIASQLKKIDDHTVQVQWSADVSPA
                     LALNILSTPIASIVDEKLVAPNAKNNDFGNEWLKMHSAGSGAFKMRTYQPHQAIVMEA
                     NASSPTGAPKLKNVIIKNVPDPATRRLLIQQGDADMARDLGADQIDALQGKPGVKVMS
                     IASAEQNYLAFNTGNKDNPLMSNPAFWEAARWLVDYDGITKNLLKGQYFTHQSFLPVG
                     FPGALEETPFTFNPAKAKEILAKAGIKDPHFTLDVENKPPFITIAQSIQASFAQGGVK
                     VDLLPAAGSQVYSRVRAHQHQGAIRMWLPDYFDAHSNASSFAYNDGKSSTVAGLNGWK
                     IPELNKETLAAIAEPDSTKRLDLYKKMQQELQRSSPYVFIDQGKTQIVMRDNVQGYQQ
                     GLNADMVWFDQVTK"
     gene            complement(5532..7679)
                     /gene="fdhF"
                     /locus_tag="ACHJ8C_002924"
     CDS             complement(5532..7679)
                     /gene="fdhF"
                     /locus_tag="ACHJ8C_002924"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7260..7262),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFO5552748.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAENAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRVAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(7877..9343)
                     /gene="mdtP"
                     /locus_tag="ACHJ8C_002925"
     CDS             complement(7877..9343)
                     /gene="mdtP"
                     /locus_tag="ACHJ8C_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO5552749.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQFNDPQLDALIQRTLSGSHPLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQNKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETREFLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQTAPVVEKK"
     gene            complement(9340..11391)
                     /gene="mdtO"
                     /locus_tag="ACHJ8C_002926"
     CDS             complement(9340..11391)
                     /gene="mdtO"
                     /locus_tag="ACHJ8C_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO5552750.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTMQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEAVVRLTLWCI
                     VVSLYPTLLMTLIGVLWFPSRAIMQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHSITEGQCWQSDWRISESEAMTARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVVLERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPAKEQAQGAT
                     Q"
     gene            complement(11391..12422)
                     /gene="mdtN"
                     /locus_tag="ACHJ8C_002927"
     CDS             complement(11391..12422)
                     /gene="mdtN"
                     /locus_tag="ACHJ8C_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO5552751.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQASDTLRRTEPLLREGFVSAEEVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTLATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(12441..12716)
                     /gene="ytcA"
                     /locus_tag="ACHJ8C_002928"
     CDS             complement(12441..12716)
                     /gene="ytcA"
                     /locus_tag="ACHJ8C_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO5552752.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRQIIQRTNINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(12925..14910)
                     /gene="yjcS"
                     /locus_tag="ACHJ8C_002929"
     CDS             complement(12925..14910)
                     /gene="yjcS"
                     /locus_tag="ACHJ8C_002929"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO5552753.1"
                     /translation="MNNSRLFRLSRIVIAFTAASGMMINTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDALRGFIAPLLDEGILRDANGKIYYRANDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMAVRLDSAKA
                     AGKNISLNFNMNNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            complement(15183..16112)
                     /gene="alsK"
                     /locus_tag="ACHJ8C_002930"
     CDS             complement(15183..16112)
                     /gene="alsK"
                     /locus_tag="ACHJ8C_002930"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0019316 - D-allose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HFO5552754.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLYCEKKRTAEVIAPD
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQSRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPYQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQSCAKVP"
     gene            complement(16096..16791)
                     /gene="alsE"
                     /locus_tag="ACHJ8C_002931"
     CDS             complement(16096..16791)
                     /gene="alsE"
                     /locus_tag="ACHJ8C_002931"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HFO5552755.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHGMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKVAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            complement(16802..17782)
                     /gene="alsC"
                     /locus_tag="ACHJ8C_002932"
     CDS             complement(16802..17782)
                     /gene="alsC"
                     /locus_tag="ACHJ8C_002932"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HFO5552756.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSS
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGI
                     DPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            complement(17761..19293)
                     /gene="alsA"
                     /locus_tag="ACHJ8C_002933"
     CDS             complement(17761..19293)
                     /gene="alsA"
                     /locus_tag="ACHJ8C_002933"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HFO5552757.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVTNLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHDTVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNMAISRSLKDGGYKGAMGLFHEVDEQRTAENQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCRPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            complement(19420..20355)
                     /gene="alsB"
                     /locus_tag="ACHJ8C_002934"
     CDS             complement(19420..20355)
                     /gene="alsB"
                     /locus_tag="ACHJ8C_002934"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HFO5552758.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIGKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            complement(20414..21304)
                     /gene="alsR"
                     /locus_tag="ACHJ8C_002935"
     CDS             complement(20414..21304)
                     /gene="alsR"
                     /locus_tag="ACHJ8C_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose utilization transcriptional regulator
                     AlsR"
                     /protein_id="HFO5552759.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
BASE COUNT         5157 a         5585 c         5424 g         5204 t
ORIGIN      
        1 agagacagta taaagtgttc gggtggacaa aaaaacaact gtaaaaagac ctgaaagcag
       61 aatgaattaa tgcaatgtca ggttttttaa tccatccgat gttccccctt ttccatggcg
      121 tcgcgatcaa aaaatcgctg aatttttccc tcggacatca atgccgctcg atcagacata
      181 tgcgcgatga catcggcatc gtgactgacc agcagatatg tcatttggtg ttccagctta
      241 agttgattca acaaattaag gatttccgct tgtaccgaca tatctaacgc cgaggtgggt
      301 tcatccagca gaagaagttg tggtcgtaac aacagggcgc gagcaataac cacacgttgc
      361 cgttgaccac cggaaagctg gtgtgggtag cgacgagcgg tgtcgaaagg caagccaacc
      421 tgttccagtg cagtctggac gcgtttttct atttctcttt cgccacgaat tttgagtggt
      481 tcagccagcg ttcgccacag agtatgattg ggatgcaacg aggcataggg atcctgaaac
      541 accatttgca cattgcggcg gagatcgccc tgaaaacgca ttcctggttt gatggtgtgc
      601 ccaaacagtg acacctgacc gtgccagtcg cgctgtaatc cagcaattac gcgcaaaatc
      661 gtggattttc cgcagcctga ttcgccaatt aaactaaatg tctctccggc attaaccgaa
      721 aaactggcgg cagaaactgc ggttttctca gcaaaagtca cctgtaagtg attaatttca
      781 acaatcgtca tggtatttct ccggcgtcat tgccgtcctg tccagcgtcg gcagcatctg
      841 cccgtaagtt tgtgcatttg ggcggcaagt ccacagtgtg cgcgtgtaag gatgcgttgc
      901 tgtaggtagc gcagcagcgt gcatttcatc caccttattt ccctgataca tcaccagcac
      961 ccgatggcaa aattgggcaa ctaatggcag gtcgtgactg atcagcaaca ttgccatctg
     1021 acgttgttca cactgggtaa ccagcagttc cagaatctga ttacgcaggc gcacatccag
     1081 tgctgatgtt ggctcatcgg caatcaatac ccggggatcg ttgatgagcg caattgcaat
     1141 catcactcgc tgccccattc cgccagaaag ttcacgagga tagctttgca gtactcgcgt
     1201 attgaggccg acagcctgaa ttgcggtgtt tattttctct tctttctcgc ggcggttcag
     1261 acgttgatgg agcgtaagcg cctcttccag ttgcgctttt actgtcttta ctggattaag
     1321 cgcgtaacgc ggatcttgca ataccatcgc gatatcgtta ccgcgcaact gttgccatcc
     1381 acgcgcgtta agggtgagtg catcacgccc cagaatgtta agtgtgtccg cggttactac
     1441 acccggttta cgcaccagcc ccatcagggc gcgggcggtc atggacttac ctgaacccga
     1501 ctcaccgacc agcgccaggc attcattgcc taatgtaaaa ctgacattgt tgacgacccg
     1561 ggactccgga taatcgacgc agagactgtt gatactaaga cgtgagtcag tcatgttgag
     1621 gctccagaac atcgcgcaag ccatcaccca gcagattaaa ggccagactg gcaattaaga
     1681 taattgcgcc gggagcggcg gcaatccacc attgatcaaa aatgacctgc ataccgtcgg
     1741 cgatcatggc tccccattcc gccattggcg ggcgagcacc aagcccaaga aaacctaggc
     1801 cagcagccgc cagaatgata cccgctaaat ccagcgccag acggacaatg gcagatggca
     1861 ggcacaatgg taaaatatga ccagccagca gacgcctgcc gcggatgtcc atcatttctg
     1921 cggcggcaag ataatcactg tgtcgcagac gttggatctc actccgtgcc tgacgcgcat
     1981 aagcaggcca ggtggttaat gccagcgcca gtgcgccgtt aaccagcccc ggccccagca
     2041 tggcaacaaa agcaaaagcc agaattaaac gcggcattga cattactacg tcagtaaaac
     2101 gcatcagcac gcgttccagc cagccgccgt agtacccggc taaaatgcca atcagcagac
     2161 cgaccgggag tgtaatgatt gtcaccaatg ccaccagccc cagtgccggg cggctgccgt
     2221 agattaaacg tgaaagtagg tctcgcccgt aactgtcagt gccgagccag tgctgacgac
     2281 tcggtgcttg taaccgcgcc gccgcatcct gccagttagg atcgtgaggt gccagccacg
     2341 gcgcgaagag cgcaattagc atcaataaca ggataattat caacccgcaa aacgcggcgg
     2401 gtgaatggcg taaacggcgt aaaaagagaa acatcggcat cagcgtaacc tcggatcggt
     2461 cagccggacc agcaggtcgg ttaagttatt tatcaacaca aagcagacgc caatcagcag
     2521 cgttcctccc atgattgctg tggtatcacc cgcgaagagc gctgtagtga ggtagcgccc
     2581 aatacctggc catgaaaaga cggtttctgt cagtaccgcg ccttccagca ttgatgtata
     2641 ggcgagggcg ataacggtga gcaaggtgcc gcgaatgttg ggtaaaacat gacgtagcaa
     2701 aattgtcatc tcgctggccc ctttggcgcg cgccagcaga atatattctt tattcatttc
     2761 gccaagacag gcggaacgag tcaggcgcgt aatgctggcg agcgagaagt acgccagtaa
     2821 taaaaccgga agtatcaaat ggttgattgc attggtaaat gcctcgcgat cgccagaaag
     2881 ccacgtatca atcagcgcaa atccgctacg gggttctatg gtgaattgat aaatatcatc
     2941 cagcctgccc ggcccggcac tccattgcag tctggcgtag aacagcgcca gcatcagcag
     3001 accaagccaa aaaacaggga cggaatttcc cagtagagtt aatgttcgca cggtgaaatc
     3061 cagcggagaa ccgacataac gggcgcaaag tacacccgca atgacgccga caacagcacc
     3121 gacaataagc gccaacgttg ccagttcaat ggtcgccggg aatgctgata gcaagtcctg
     3181 gagtactggt tgcccggtgg agctggcgat gcccagatcg ccgtgtgcca gattcatcag
     3241 gtaatgccag aactggacgg ctaacggctg atccaatcct aactggtggc gtacctgatc
     3301 ataggtggac tggctggcgt gatcgccaac gatttgtagt acgcgatcga ccggtgagag
     3361 agccgaaagc gcaaaggtaa tcagcagcag gccgagtagc gtcagagcaa cggtaaacag
     3421 cccttgcaac agcgcgagca gagggcgctt gcctcgccat ctcgcgcgag tggaaaaaac
     3481 gacagacatg atctttccat acagtgataa atcagcggct ggcctgcaat acaggccagc
     3541 cctggtaccg cgcccggtaa cagggctgga cgcgagttgc atacagggtt atttggttac
     3601 ctgatcaaac cagaccatat cggcattcaa cccttgttga tacccctgca cattatcacg
     3661 catgacaatt tgcgttttgc cttgatcaat aaagacatac ggggaactac gttgtagttc
     3721 ttgttgcatt ttcttataca aatcaagacg tttagtgcta tcaggttcag cgatggccgc
     3781 cagcgtttct ttgttcagtt ccgggatttt ccagccgttt aacccggcaa cggtgctgga
     3841 tttgccatcg ttataggcga aagagctggc gttggaatgt gcgtcaaaat aatcaggcaa
     3901 ccacatacga atggccccct gatgctggtg ggcgcgaacg cgagagtaga cctgactccc
     3961 tgcggctggc agcaaatcaa cttttacacc gccctgagca aagctggcct gaattgactg
     4021 cgcgatggta ataaatggcg gtttattttc gacatcaagc gtgaagtgcg ggtctttgat
     4081 tccggcttta gcgaggatct cttttgcttt ggctggatta aaggtaaacg gtgtttcttc
     4141 cagtgctcca ggaaagccca cgggcaggaa actttgatgg gtaaaatatt gtccttttaa
     4201 gagatttttg gtgatgccgt cgtaatcgac caaccagcga gcggcctccc agaacgcagg
     4261 attactcatc agcgggttat ccttgttacc ggtattgaag gccaggtagt tctgttcggc
     4321 ggaggcaatg ctcatcactt ttacgccagg tttaccctgt aatgcatcga tttgatcggc
     4381 tcccagatca cgagccatat cagcatcacc ttgttggatc agcaggcgac gcgttgccgg
     4441 atcgggaaca tttttgatga tgacgttttt cagtttcggg gcgccggttg gtgagctggc
     4501 gttggcttcc atcacgatgg cctgatgcgg ttggtaagta cgcattttaa acgcgccgct
     4561 gccagcggaa tgcatcttca gccattcatt accaaagtcg ttatttttgg cgtttggcgc
     4621 aaccagcttc tcgtcaacaa ttgaggcgat tggtgtggag agaatattca gtgccagcgc
     4681 cgggctgacg tcggcactcc attgcacctg gacggtatgg tcatcgattt tcttcaactg
     4741 gctggcgata ttttccggct gccatcccag cacattgaga ataaacgcac cggatttatt
     4801 cagtgtgaca gcccggacat aagagtaaat cacatcttcc gggcgtaccg gattaccaga
     4861 ggcaaatttc gcatcgcttt tcagttttat ggttagcgtt ttggctgcgg ggtcagcctg
     4921 ccaactttcc gccagaatcg gagtgatttt cgtcggatca ttacggtccg gttgtactac
     4981 gcgttgatac agacttggta cagtctgaat acttgagagt tcattagctt ctgccgggtc
     5041 cagactcaca atatcatcca gtccctgggc tacaaccaac gtattaggcg gtgtagcggc
     5101 aacggttgtg gcggaaattg tgcttaaaac cagtaaaggt aatagttttt taatcatatg
     5161 cttcccaatc cctgaaatta ctttgaggtt attaatatgc tatcagacgg ggtttcgctg
     5221 cattaatcgc gccagaatat cccgttaata gaacgagtta aacggtcgat gatttctcac
     5281 cttaccgaaa ttttgtttat caaatgtaaa gtattattac gttaggccgc gatgagaatt
     5341 gagtaaagat aattctcatt tgcaatcgtg actggaatag aagaacaacc gagaagacgt
     5401 cgctaataac caggatgggc tggtttgact gcgcaggtat gtataaaaaa atccggaaac
     5461 agagagtcgt ttccggattg atacaaaggc gttgggtaat atcgccgctt ttaacgtgag
     5521 tttacgcggt attacgccag tgccgcttcg cgcaggcgag ttttcaactt gttgtactcg
     5581 tcaatcacgt actgctcggc ggcgcgctga tcggcgatcg gctcgacgcg cacggcgcag
     5641 tatttgtatt ccggcgtttt cgtaatcggg cttaagtttt cggtcaccag ctcgttacag
     5701 gcaccaatcc accactggta ggtcatgtaa atcgcccctt tgttcggacg atcgctgacc
     5761 tgcgcacggg tgataatttt gcctttacgc gagtgcaccc aaaccaatgc ctcatcttca
     5821 atacccagac gtttggcgtc ttcggtattg atttgtgcgt agccaggttc atcagccagc
     5881 gctgccagtg ccgcacagtt accggtcatc gaacggcaag agtagtgacc aacttcacgc
     5941 accgttgaca gtaccatcgg gtactcgtcg gtgagtttgt cgattggcgc tacccagtcg
     6001 caggtgaaga actgcgccag accgttcggg gtatcaaact tctctttaaa cagataagac
     6061 gtcccctgat cggcatctga agtatcgcgg caaggccact gaatgaagcc cagttcgccc
     6121 attttctcgt aagtcgcacc gtagaaatcc gggcacagat gacgcaactc gtcccagatc
     6181 tcctgggtgt tgttgtagtg catcggataa cccatacgag tggcaatttc actgatgatt
     6241 tgccagtccg ttttcagatc ccacttcggc tcaaccgcct tgaagaaacg ctggaagcca
     6301 cggtcagccg cagtaaacac gccttcatgc tcgccccacg acgttgacgg taaaatgaca
     6361 tccgccgccg acgcggtttt ggtcataaag atgtcctgaa cgataaccag ttccagatct
     6421 tcaaaggctt tacgtactgc cgacagttcc gcgtcagttt gcagcggatc ttcgcccata
     6481 atgtacgccg ctctcacttc accatgcgcc acgcggtgcg gcagctcgct gatgcgatag
     6541 ccggtatgag caggcaggct ttccacgccc caggctttgg cgaatttctc gcggttagcc
     6601 ggatctttca cgtactgata gcccggatac gtatccggca gcgcgcccat atcgcaggca
     6661 ccctgcacgt tgttctgacc acgaaccggg tttacacccg catgtggctt accgaggtta
     6721 ccggtcagca tcgcgaggct ggtcagagaa cgcacggttt ccacgccctg atagaactgg
     6781 gttaccccca tgccccacag gatggcggcg ttttccgcct gggcatacat ccgcgccgcc
     6841 tgacgaatct cactggcgct gacgccggtg atatcttcaa ccgactccgg cgtgtagcct
     6901 tcaacgattt tacgatactc ttcaaagcct tctgtacggg aagcgacgaa cgctttgtcg
     6961 tacagatttt cttcaataat gacatggccc atcgcattca acagcgcgat gttcgagccg
     7021 tttttcaatg caatgtgcat gtcagcaatg cgcgcagttt caattttgcg cggatcgcag
     7081 acgataattt tcgccccgtt acgtttagcg ttaattacgt gattcgccac gattgggtgg
     7141 gaatccgccg ggttataccc gaaaacgaac actaaatcgg tattatcaat ttcgttaata
     7201 gcattgctca ttgcgccatt accgaccgat tggtgcagac ctgcaaccga tgggccgtgt
     7261 cagacacgag cgcagcagtc aacgttattg gtaccaataa cggcgcgcgc aaatttttgc
     7321 attacatagt tggtttcgtt acccgtacca cgcgaggagc cggtcgtctg gatggcatcc
     7381 ggaccgtact tctctttgat ggcgctcagg cgctcggcaa cgtaattcag tgcctcatcc
     7441 caggaaacag gttcgagttt gccgccacgc tggcgacgga tcatgggggt tttcaggcgc
     7501 ggggtcagga tctgggtatc gttaatgaag tcccagccat aataaccctt cagacacagg
     7561 gtaccctggt tggttttccc ctgcgctgcc tccgcccgga cgattttgcc gttatcgacg
     7621 accaggttga ttttgcaacc tgatgcgcaa taggggcaaa ccgtgacgac ttttttcatc
     7681 ggtctcgctc cagttaatca aatcacgcat acgcgctctc gactacagta tgcatctttt
     7741 atgccacatt ttatttgggg tcattccctg atattacggg cactatttat tcaaaactct
     7801 gacgaaaaac aggctgtcgt cagttttgac gtgacgaaac gaaataccgc gtgacagcca
     7861 tcacgcggta gacattttat tttttctcga cgacgggagc cgtctgatac ccgccgccca
     7921 gcgatttcat caactgaatg ctttggatca cccggcggct gtccagcatc agtaatgaca
     7981 tctcctcggc aagcactggc aaccgggctt cggtggcctg taagcggctg gttaagccgc
     8041 gctgataggc ggcctcggct gcgcgctggg tgaagcgcgt tgcttctacg cgttcagcct
     8101 gcatctctcg ctcgtcgttg agcgtttgca gacgcgtgcc gttgacggca acgtcacgca
     8161 ccgcgttcag tactgactgg ttgtaacgtt caatcatcat gttgctggcg gcgcgagtgc
     8221 cttcgagatt ggcattcaac cgtccaccgt caaacagcgg caatttcaga cccgggatga
     8281 agttgaactg gcgactggtt tttttgaata aggtatccag gtggatggag tccagaccga
     8341 aaaacgcttt gatatcaaag ctcgggtaga acagcgcccg cgcggaatcc acctgatcta
     8401 atgacgcctg aacataccag cgcatggctt gcagatccgg gcgtctggcg agcaactcat
     8461 aagagagcgt tgccggaatg ccggtctgta ctcgtggtaa tgccaccggt ttgatctccg
     8521 gcatatcgct ggctcccgcg ccaatcaatg cacgcagaaa ttctcgcgtt tcagtgattt
     8581 gccctttgac ggcagcaatt tgtttatcga ccgccagaat ctgtgcccgc gcgccgtgga
     8641 aaggcacttg cgcttccaga ccgtgcgcca ctttattctg gtgcgctttc accgcgtaat
     8701 caatcacatc gcgagtttgt tctaacagat cgagcatctg atagctggcc tgcatactgt
     8761 aataaagctg cgctacgccc gtggtcagcg atagctctac tgctgcggtt tctgccagcg
     8821 cggcattatg cgcgccaatg gcggcagcaa ccgctgagcg atgcacaccc cacaaatcga
     8881 gatccagtcc ggcaaacaaa ccgactgtgg cttccgtata gtacggcccg tccataccca
     8941 gtgctggcgc atccatcgca taagggctta aaaagccgtt cgccgagacg cgttggcggt
     9001 tgagcatccc taacgctgcg acctgtaatt gtgaaccggc atctaacaaa tcggcctggg
     9061 actgcgcttt ttcttcccgc agtttcgctt cggcgagggg gtgtgaacca cttagcgtcc
     9121 gttggatcag cgcatccagc tgcgggtcgt taaattgttt ccaccactgc gcctgcggcc
     9181 agccggagct ggcaagatga atatcatcgg ccagtttgat ttgttccggt ttgagctgtt
     9241 gatgaggcgc agaatcctta cgtaccaggg cacagccgga aatcagcgtc gtgctgccga
     9301 gaatgctgca caacagcaga cgtgaaagtt gacgattgat cattgcgtgg ctccttgtgc
     9361 ctgttccttg gcgggcgtta atgccggggc tgtccagtct ggcaagcggg cgaaaagctg
     9421 gcagacgtgt tgctcctgtt ttaataaggt cggcagaatg gcctgcgagg cgggtgtttc
     9481 ttcaagcgtt atttgaggag aacggctgag tgcggttgcc agaccggcgg cgtatttctc
     9541 cagggcgtcg gcaaactgac ctgctctgtc cggctgtagt gcgttatcca gcgcctgcgc
     9601 cgagttccag gtggcatccc aggcgtgaag aatatcgcgg ccctgacgaa taaccgtttg
     9661 cgaacgttca atcagtaatg cgcgttcttc gctgtccagt tgacgctcca gcaccacgcg
     9721 ttggcacatc tcttcacagg cattaaacgc cgcatgcaga cctatgcgaa tttgcagata
     9781 agtgcgcaga gccgtgactt cctgctggcg tgggatccgc attactttac tcaacatacc
     9841 cagcgcgcca gccagttttt gcggcagcgt gcgcgcttcg ctttcaggcc agacaaaggt
     9901 gtaaatcacc gcggacacca cggtaccaat gatgataccc agggcgcgat cgcgaatttc
     9961 caccaggtcg tacaccgggc caaaaacgtt ttcgagcgtg gcgagcgcga aggtgaccac
    10021 catctgtgtg ccgatataag aagagcgttc agagctggtg gcaatccatg cgcccaacag
    10081 gaaaatcggt gccagcacaa acagcaattc gacaatattg tccagccagg gcatgaccaa
    10141 taacgtgaat aacagcgcca gaatcgcgcc gcaaaaggcc ccgccaaaac gcagcaccat
    10201 cttctggtac gacgaaccga cattgggatt agcgacgatc acgcaggtca gcatacaggt
    10261 gtgaatgcct tcccagtcca cgccgctgta gaaggtgtaa cagatcaaac aggcgagcag
    10321 cgtttttacc gcgtagcgca tatagtctgg attggtaaag gcatcggcga ccattgatgg
    10381 cggcttggct gcaggcgtag gcggcgtatt cgggtccatc tgacccagtt gcaataacgt
    10441 ctggcagata ttctccaggt tacattcccg tgccgtcatc gcttcacttt cactgatccg
    10501 ccagtcgctt tgccagcact gaccttcggt aatggaatgc tgcagcttgt tgatttctga
    10561 agctaatttt tgtcggaatt caataattgc ctgagaatca gcaaaagagg tgggatcgta
    10621 gcgattcagt gtcgagtaaa tgtaggttac cgttgccacg cagctttgcc accatgcgct
    10681 ctgggttcgc cagttggcat cgtccgcgag gcaaaagaca ttgagttttt gcagcgccag
    10741 cgcctctctt tcaatccgcg tttcgggtag cggtgcgagg ctgtccgtca ggtggctaat
    10801 ggcatcatca agccgatcat taagcgcctg atgcatctgc ataatggcac gactgggaaa
    10861 ccacagcacg ccgattaacg tcatcagcaa ggttggatag aggctaacaa cgatacacca
    10921 cagcgttaag cgcacgaccg cttccggata gtcgagcatg gcggggaagg tttgcccgta
    10981 aatagcgaca atggcgacgg cgaaaaagac cagccccaac cgatgggtgc gcatcaaaaa
    11041 catgcagccc atcaggatcg gcccggcgat gatcaatcgg atcaacggtt cgccatatga
    11101 ccatttgtag atcaaaaaca ggctgccgat ctccagcacc gtggcaacca caaacaagat
    11161 cgcgacaaat ttggtgtaaa acgcgttcga ctgaataccg taaaacagca ctgccagtga
    11221 taacgccaca aaagggatct caaaagtcat cgagatcaga atcaccagca ggcaacctac
    11281 ccagagttgc atagtctgtg gcacgcgccc tggtcgccgc tcgcttaact cttcatgaaa
    11341 gaacgccagc agcctgacca ccggtaatgg cagggagttg agcgcgctca ttattgcggc
    11401 tcaagattag cgactgccga agcgccgatg cggaacattt ccgggtcagg tttatcaacc
    11461 atgattttga ccggaaaacg ctgggcaacg cggacccagt taatagaacg agacactttc
    11521 ggcaggccgc ccagcaccag gccgccgtca tccggtagca cgccgtagcc aatcgaatcc
    11581 actttaccct cgaaggtttt gccgctgtcg ctcatcaggc gaatggttgc gagtgtaccc
    11641 gagcgaatat ttttcagatc agtttcgcgg aagttggcaa tcacgtacca gtgacgagtg
    11701 tcgattagag tgaaaatagg gcgcatcgca gatgcaaact gcccgacgga ggttttgagg
    11761 gaaatgaccc ggccatcaaa cggcgcgcga acggtcgcca tttccagatg cagtttggtc
    11821 agggcaatat ccgcttccac tgccgcacgc tgcgccacca gcgcatccac gccgctgacg
    11881 gcgctggcgg ctgactgcgc ctgcaacaat acggcattaa ggtccgcttc tgccgcacgc
    11941 tgtgcggttc ttgcacggtc aacctcttcc gcagaaacaa aaccttctcg cagtagcggt
    12001 tcggtacggc gtaacgtatc gctggcttgt ttcgcggcgg cacgggcttt ttctaccgtg
    12061 gcattaaccg agtcggcacc aaactgttgt gcgtcaacgc tacgctgggt gagcataatt
    12121 tgcttatcca gcgccgcgag ggaggcttca gctttcgcca gattggcttc gtacgggcgc
    12181 gggtcaatgc ggaacagcaa atcgccctgt ttcaccgcct ggttgtcggt gaccgccagt
    12241 tctacaatgc gaccgctgac ttccggcacc acatcaatgg tatctgctga cgcgtaagcg
    12301 tcattagttg atggcgcact gtctacgcgc cagataacga aaacaagggc aaccaacgcc
    12361 aacgcaacca ctaacagagc agggaattta ctgcgaggag cttttttcgg cgtactttcc
    12421 atgagcgcgt ctcaaataga ttagaaaaat gccagccaga acagcatggc gtagagagca
    12481 aaaagggcgg tataaataat tccgacaaat gccagattga tatttgtccg ctgaataatt
    12541 tgcctcgtga tgagcgtcaa aataaggctg gcaatggcgc agaaaaacca gctgggataa
    12601 taagcgccga tcaccgggat tgccggagat aatgagcatc ccgaaaccat gaagacggga
    12661 attatccagg ctgttttttt gagttgcatc gccatgcgag agagaactgt tggcatatta
    12721 aatacatcct gtctgctgcg aacaatatct gtagtgaata tcaatatcca ttgaaggcgt
    12781 cagtatggct attttctcgc tgaataaaaa tagctttatt cagccaccgc attcataact
    12841 tatatatttt atgtattaat ttgctgtata tatttttaaa ataaatgctt ttgggagctt
    12901 cagcgccccc aaaagcgagt gacattaatt tggggttacg atattcaccc acaaatcgaa
    12961 attatccaga caggcgataa tttcttccag tttcgcgcca ttgccaataa ttttggcttt
    13021 cttcgctttt accagatcgg ccatttttgc ctgtccggtc agcacggcgt gcagatcttc
    13081 acggctgatg tagaaagagg cgtcagcttg cggttgcagt gttttacggt agttaagcac
    13141 gctatcgttc agcgtcaggt tgaggttatc gccgttgttc atattgaagt tcaggctgat
    13201 atttttaccc gcggctttcg cgctatcgag gcgaacggcc ataaagtcga acagcatttc
    13261 gaccgacatc ccacgaatgg tgtccgggga accggtggtg ccgtggctga acttatgcac
    13321 cccttcgcgc agctctttcg cgccggtcag gtagaaaccg cgccatgtgg cggactcggc
    13381 ctgatagccc agctgttcaa agttattcgc ttgcagattc ttcgcgacct ggtcacccgg
    13441 gttggcggca atcacctgtt tcagcagttc tgccgaccag cgataatcac cttgcttgtt
    13501 cgcttcttgc gccaggttga tgacgcgggc agaaccgccc agcgcctgca cgtaacgttt
    13561 acccatctcc acctgaccat acggatgcag gttagccggg ttaccgtcgt aatagccaag
    13621 atagaagtta tacaccgcgc gggcgttgtg gctgacagaa ccgtaataac cgcggctggc
    13681 ccagttattg gcaagcgcag gcggcagctt aatcatgtcg ccgatttcat tcatggtgta
    13741 gccctggttc gccaggtgca gggtctggtc gtgaatgtac ttgatagtat cgcgatattt
    13801 accaatataa tcattgatat gcttattgcc ccagaccggc caggtgtgcg gcataaacag
    13861 tacttccgcg tcgttacccc acatatccaa cgtttcgttc agatactcgg tccacttgct
    13921 ggtgtcgcgg gttttcgcgc cgcgcagagt gtagaagttg tgcagggtat gcgtggcgtt
    13981 ctcggcggta cacagggctt tcagggccgg aatatagaag tgcatttcgg ctggcgcttc
    14041 gctacctggg gtcatcagga agtcaaactc caggccgtcg ataatcatct tctcgccagt
    14101 tctgacgata gttttagtcg gtgcaataat gctcgggtcg cccgttgcca gcgtcacgcc
    14161 aaggccatta cccacattgc cttgcgcgtt gtgcggcagt aacagaccgt aagagtagag
    14221 cgcacggcgg ctcatgatgt tacccgccag cacgttttcg ctgatggctt cgtccataaa
    14281 gccagctgga gcaatcacct gaactttgcc ggatttaaca tcggcttcag agatgatgcc
    14341 tttcacgccg ccatagtggt cggcgtggct gtgagtgtag ataacggcaa caatcggttt
    14401 ttgcggacga tgctggaagt aaaggtcaag tgcggctttt gcggcaggtg gcgtcaccag
    14461 tgggtcaata acaataatgc ctttctcacc ctcaacgaac gtaatgttag agatatcctg
    14521 gccgcgcacc tgatacattt tatcggtgac tttgaacagg cctgaaatgc cgttgatctg
    14581 cgactgacgc cacaggctgg ggtttacggt ttccggcgca gcggcattaa tatcaaattt
    14641 gtaatcattc gcgcggtagt aaattttacc gttcgcatca cgcagaatac cttcatccag
    14701 cagcggagcg ataaatccac gcagagcatc gtcaaaatcc tggcgatcgc taaacggtaa
    14761 tgatttggcg taattctgat taacctgttg ggtatattga gtggcagctt tcgcttcatc
    14821 tgttgcgtaa gcggtattta tcatcatgcc gctggcggca gtgaacgcaa taacaatcct
    14881 gctcaaacgg aataaccgag agttattcat ataaatctcc atttatcatt tatgtcgtaa
    14941 atatgtaatc catgcaaaat acgtcattta aagctggcgt tatcataatt ctgaaattgc
    15001 gactttaata agtggaagtg tgagcggaac gatccatttt attaggcatt tgtgctgaag
    15061 agggaaaatt acagtagaac tatcattgct gggaataata ttggctgttt tatgtaatta
    15121 ttatatatat tgtacctgtc gttggcacag cgggacattg cgctactgtg ccaacgcttt
    15181 actcatggga ctttagcaca ggactgtggc aaaaaacgtt gatgcgccaa tattgctgca
    15241 ccctgagcgc cattaaagtc agaagatgag gcggcaataa agcgcacgac ctgatacggc
    15301 agtggacggc gcaggtactt ttgggtcatg gcaatgagag tctcgcgtgg gaaggcgggc
    15361 atatccatca cgccaccacc cagaatcacc gcatcgggat cgaacagatt aatgctggtg
    15421 gcaatggctc gtgccgcgtt ttcaagcaga ctctggacga aaggggcgtt ttccgcatgg
    15481 acgaaaagat cgctcaatgg gtaatttcgg gactgttgtt cgtaccagcg tcttagcgcc
    15541 attccagagc aattggtttc caggcaccca ggattaccac acgcgcagtg ttgggtcata
    15601 tctcccaggg ggatatggcc cagttcgcct gccacaccgt gtgcacccgt ccacggcgca
    15661 ccgttcatcc acactgcgaa ccccatcccc gtaccgagat aggctgccag aaccagttgt
    15721 tgcgtaaggc ggttttctac tacgtcccag gagagttgca ggttaacgtc gcgggaaaac
    15781 tcaaccggac aattcagcgt attttcgagc ttatcggcga gatcatataa atctgccgct
    15841 gttaacggca ggttaggcgt agaaataatg gtgcgtttat ctttactgac cagcgccgga
    15901 aatcccatca ccagaccatg acagcgagcg ttaaagcgcc tgagttgctc gtcaatcatt
    15961 tcgccgatac ccgacaccag gtcgggagca ataacttctg cggtccgctt tttttcgcag
    16021 tatagcgttt caccttctgc tgtccgcaga caaaagcgga tatgcgttgc ccccatatcc
    16081 acgcccgcta cgacgttatg ctgtttttgc atgaggctgt acctcgcttt ttgcagccag
    16141 aatctgcgca gtcataattc tccatgcttc gtcgatattt tccgcatgat taaacaggcc
    16201 ggaagtgccg acgataaaga catccgcccc tgccgccatt agtttttcgt aagttgcctg
    16261 gttgcaggaa ccgtccacct caatttcgta ctccagacct tctcgttcac gccatgcctt
    16321 cagttcggca actttatcca gcatttcagg aatgaagggt tgtccggcaa aaccgggatc
    16381 gacagtcatg accgtaattt tatcagcctt atggatatag tatttcatgg cctcaaccgg
    16441 cgtttccgga ttaagaatca gccccacttt catgccatga cggcggattt catcaatcag
    16501 gcggaatgcc tgaccgttga tagtttccgg atgcaaagtg atgaaatcag ctcccgcacg
    16561 tgccagttga gcaatgtaat cctgcggacg cgttaccatc agatggcagt cgagcggttt
    16621 actcgccagt ttttttacct ggcttacgaa gaacggtgag agcgtcagat tcggcacaaa
    16681 gtggccgtcc atgatatcga tgtgaaagta atcggcatgg ctgtcgataa attcgatctg
    16741 ttctttaaat ttcagcagat ccatacacat taacgagggg gagattttca tgttcaattc
    16801 cttacttact gataagacgg tcaagggcga cagccgcgat aattaatccg cccatcacca
    16861 ccagttggta ataggtttgt acctgcaaaa tattcagacc gttgttgatg gtgccgatga
    16921 tcaacccgcc aatcaccaca gagaaaatgc gccccttgcc gccgaagaaa ctggtgccgc
    16981 caatgatggc gctggcaatg gcataggttt caaaacccat accggcaagc ggttctgcgg
    17041 caccgagtcg tgcagttgag acgacgcctg ccagacctgc acaaacaccg gagatgataa
    17101 acaccaccag gatgtggaat ttcacgtcaa tcccggaata gaacgccgag tttttgttgc
    17161 cgcccagtgc gtagatgttg cgtccgagcc gcatacgcgt tgtcagaaac caaaggatga
    17221 gcgcgacaat tagcgagaag ataacgggga caggtatccc aattacgctg gcggcaaaga
    17281 agttcacgaa gtcaaatgag aagccgtata ccgagttggc atcggagatc accagcgtga
    17341 tcccacggaa aatagcgttg gtgccaaggg tgataatgaa cgggtgtagt cccgtccagt
    17401 tgaccaggca gccgttgatc gcccccagtg cgccgccaac cagtacaccg ccaattagcg
    17461 ccgcgagaaa cggatcaata cctgccaaca tcagtttagc ggtcaccata ccggaaagcg
    17521 ccagaatcgc gccaaccgaa aggtcgatac cggcgaccag gatagcgaag aattccccca
    17581 tgccgatcag taccgtcacg gagctttgca caaaaatctg ggtaatatta ttggtggtca
    17641 gaaaatattc tgacgacagc gaaccaaaaa tggcgacgat gatcgccagg ataaaaaagg
    17701 tgccgtattt atcccagaac agcgcaaagt tgaacggttt cttctcgctc gcttcgcttt
    17761 ttactcttgt ggtaaagccc atgccataat ctcctcttcg ctcatgtcat cgcgattcgt
    17821 caggatttgc gtcagtcgtc cttcgcagaa cacggcgatg cggtcgcaga cggtgataat
    17881 ttcaggtagt tcagatgaca ccatcaggat gacttttccg tcgtccgcca gttggcgcat
    17941 cactttgtaa atttcggctt tcgcgccaac gtcgatgcca cgggtaggtt catcgaaaat
    18001 gatcacttcc gggcgacagc acagccattt ggagatcagg actttctgct ggttaccgcc
    18061 ggagagttca gtgatgttct ggtttaccga atgacatttc agcgccagca gttcgcgttg
    18121 attttcagcg gtacgttgct cgtcaacttc atgaaacaac cccatcgcgc ctttatagcc
    18181 gccgtctttc agactgcggc tgatagccat gttctgagcg atagaaaagt tggggaaaaa
    18241 gccgttatcc cggcggcttt cagtgatgta agccatccct tttttcacgg catccagggg
    18301 agaacgtgga gagatatctt tgccattaag acggatttct ccgccagcgc gtttatcaac
    18361 gccaaacagg cagttcatca gttcagtacg tccggaaccg accagtccgg caaagcctaa
    18421 tatttctccc cggcagacgc taaatgagat atcccggacc ttttttctgt cacggctggt
    18481 gacgttgcgc acctcaaaaa ccgtatcgtg cgcaaggttg ctgacattct ccttcatcgc
    18541 gttaaaacgg ttttgcaatt cgcggcctac catcagacgg acgatatcgt catttgacac
    18601 atcgcttacc atgccgctgc aaacgctgct gccgtctttc atcaccgtat agcggtcgca
    18661 aatacggcga atttccgcca acttatgcga gatatagacg atggccgtac cctctttacg
    18721 caactgattc atgatcagaa acagatagtc cacctcttta ttggtgagtg aggaggtggg
    18781 ttcatccatg atgatgactt tggcgtcgag catcagcgtt ttggcaattt ccagcatctg
    18841 cttgtggctg atagataagt tcgtcacttt ctcatctaaa tcaactttca agcccacgcg
    18901 taataacatc attgcggcgc ggacacgcat ttctcgccag tcgataatat tgacaccgca
    18961 gatttttttc gtcagatgac gaccaatata taaattttcc agtacggtta attcatcaat
    19021 aacgctgagt tcctgataaa taatcccgat accgagttgt gccgctaatt tatgatccag
    19081 tttgttatag ttaatgttat taatggtaat ggtgcctttg gtcggctcat gtatcccgga
    19141 taacactttc attagcgtag atttacccgc accattttct cctagtaatg catgtatttc
    19201 accaggataa accgttaaat taaccgactt taatgcgtga accggaccaa aggacttgcc
    19261 gatccccgcc atcgatatat atggcgtggc cataaataac ctctctgcac gaaatatcgt
    19321 attattgcct gatggctacg tttatcaggt ctacgttcag gttattttgt tggccggata
    19381 agacacttca ttagcatcat catccggcat cattcagttt tattgagtga ccaggattga
    19441 atcgaccagt ttaaattccg gtgctttatc cagcgggata accttgccgg atttctcagc
    19501 gtcaaccatc agcttcagac ccgttgcacc gatatccgcc gggttctggg caaccgtcgc
    19561 ggtcatttgt ccggcttcca ccattttgcg ggcttccgga atgccatctg taccgacgac
    19621 caacactttg cccgtttttc cagcgtttgc gacagcctga gcaacaccca ttgccatcgt
    19681 gtcattcgcg caatagatcg ctttaatatt cggattacgt tgcaacacgt tagttgcgac
    19741 atccagtgct ttaatgcggt cccagtcggc aggctggctg gcgacaagct tgatttggct
    19801 tgcttttttg aaggcttcgg tggcgccatt acgacgtgct tcgccggagg cgttaccggc
    19861 tttaccctca ataattgcaa cttcaccccc ttcggcaccc agcttgccaa taatgaacga
    19921 cgcgcctttc gccccgacgg caacgttatc ggtggtgaca aaaccttcca cattgccgcc
    19981 agcttttttc agattatcca tgtcgatttt ttcatcgaga ttgaccagat aaatgccttt
    20041 tttccatgcg cgggcgacag gcatgaccag gttcactgag gataatggtg cgaaggcgat
    20101 acctttgtaa tttttattac tgagatcttc aaataactgc aattgagatt gaaaatcgcc
    20161 ttctgaagga gaggcaaaaa tatcaacgct gacgcccagc gtttttgctt catcttcaat
    20221 gccttttttc atatctaccc aaaatggatt ggagagtgtt ttcaatacga cggcatattc
    20281 ggcggcagca aaagcgctgg ttgacaacat taagcccacg agtgtgccgc tgaaatattt
    20341 cagatattta ttcataatca tgttctcacg gtaaggggag aggtaactcc gcgatggcgg
    20401 aattaccttt ttcttatttc agcgcgcctg gtgagaagaa atcaacaatt gcgccggttt
    20461 tttgcatatt aatattagct tgttcaatgt tgagctgggc gacagagaca aaaaacgcgt
    20521 ccagcaaagt cagttgtaat attcttgccg aggcattacg acctaataac ggtgtttccg
    20581 gggccggtga gcaaataata taatcggcca gcttcgctat tggtgaatga tagctatgcg
    20641 ttatacaaat aatctttgcc ccgttctttt ttgccagttc tacagccgct tttacatcac
    20701 tggttcgccc ggaatgggtc accaccagca ccacatctcc ttcctgtaac aacgaagcag
    20761 acatcatcat gatgtgagcg tccggatagg cctggcagcg tacgccaatg cgcaaaaact
    20821 tgtgctgtac atcagcacag atagcatttg atcctccggc accgtacaaa tcccgctgtc
    20881 tggcctgata gaaaaagcgg gcggcacggt ggatctcatc aacgttgacg atcgactgac
    20941 cttccataat ggtgcgtaaa gtgatgttaa ataccttatt caccacatcc tgcggcgctt
    21001 catcaaaagc caactcggaa ggcaatactt gttctgactg agaaaaataa tcttccagcg
    21061 cactgcgtaa gttacgaaag ccgctaaacc ccagcagctt tgatacctta acgatcatcg
    21121 cttcagatac cgccagagct tctgcgacat ctttaattgc gggtgcacaa ctcaggttac
    21181 cgggtttgag taaccactcc acgatgcggc tttcgttttc tgtcattcct tcctgcttca
    21241 ttcgcaggta aggcgctaac ccgataccgt tcggaagcgc tgaatcaaac tctgactggc
    21301 tcatcacgtt ctcttgtcct tgaattgaag acgccactat atcaccacag caagaacaga
    21361 aaagatgctg
//
LOCUS       DAZFJL010000081        21204 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFJL010000081 DAZFJL010000000
VERSION     DAZFJL010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.081"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..349
                     /locus_tag="ACHJ8C_002936"
     CDS             <1..349
                     /locus_tag="ACHJ8C_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5552760.1"
                     /translation="LNNNYREKITLEQLSKKFRASVSYICHEFTKEYRISPINYVIQR
                     RMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQE
                     ILSEFPQPVSLAG"
     gene            complement(392..1543)
                     /gene="ydiO"
                     /locus_tag="ACHJ8C_002937"
     CDS             complement(392..1543)
                     /gene="ydiO"
                     /locus_tag="ACHJ8C_002937"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO5552761.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1557..3152)
                     /gene="ydiF"
                     /locus_tag="ACHJ8C_002938"
     CDS             complement(1557..3152)
                     /gene="ydiF"
                     /locus_tag="ACHJ8C_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO5552762.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(3299..4057)
                     /gene="aroD"
                     /locus_tag="ACHJ8C_002939"
     CDS             complement(3299..4057)
                     /gene="aroD"
                     /locus_tag="ACHJ8C_002939"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO5552763.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVIAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4088..4954)
                     /gene="ydiB"
                     /locus_tag="ACHJ8C_002940"
     CDS             complement(4088..4954)
                     /gene="ydiB"
                     /locus_tag="ACHJ8C_002940"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO5552764.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4966..6231)
                     /gene="ydiN"
                     /locus_tag="ACHJ8C_002941"
     CDS             complement(4966..6231)
                     /gene="ydiN"
                     /locus_tag="ACHJ8C_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5552765.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGEHKFSTRLNTIKHRG"
     gene            complement(6458..7672)
                     /gene="ydiM"
                     /locus_tag="ACHJ8C_002942"
     CDS             complement(6458..7672)
                     /gene="ydiM"
                     /locus_tag="ACHJ8C_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5552766.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRHLPVIKKTTSSTEHRCSIIDLASYSLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALLTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFILALFIGLRSRKETR
                     HHTLKENIAPGG"
     gene            complement(7772..8128)
                     /gene="ydiL"
                     /locus_tag="ACHJ8C_002943"
     CDS             complement(7772..8128)
                     /gene="ydiL"
                     /locus_tag="ACHJ8C_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO5552767.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGNSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8264..8371)
                     /gene="rprA"
                     /locus_tag="ACHJ8C_002944"
     ncRNA           complement(8264..8371)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHJ8C_002944"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8557..9669)
                     /gene="ydiK"
                     /locus_tag="ACHJ8C_002945"
     CDS             complement(8557..9669)
                     /gene="ydiK"
                     /locus_tag="ACHJ8C_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO5552768.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKSNK"
     gene            9883..12939
                     /gene="ydiJ"
                     /locus_tag="ACHJ8C_002946"
     CDS             9883..12939
                     /gene="ydiJ"
                     /locus_tag="ACHJ8C_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO5552769.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVTTVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            12936..13346
                     /gene="menI"
                     /locus_tag="ACHJ8C_002947"
     CDS             12936..13346
                     /gene="menI"
                     /locus_tag="ACHJ8C_002947"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO5552770.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13446..13634
                     /gene="ydiH"
                     /locus_tag="ACHJ8C_002948"
     CDS             13446..13634
                     /gene="ydiH"
                     /locus_tag="ACHJ8C_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO5552771.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14183..14551
                     /gene="sufA"
                     /locus_tag="ACHJ8C_002949"
     CDS             14183..14551
                     /gene="sufA"
                     /locus_tag="ACHJ8C_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO5552772.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMIGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14560..16047
                     /gene="sufB"
                     /locus_tag="ACHJ8C_002950"
     CDS             14560..16047
                     /gene="sufB"
                     /locus_tag="ACHJ8C_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO5552773.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYHAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDN
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPLREGKEVAVDAIFDSVSVATTYRKKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16057..16803
                     /gene="sufC"
                     /locus_tag="ACHJ8C_002951"
     CDS             16057..16803
                     /gene="sufC"
                     /locus_tag="ACHJ8C_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO5552774.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTSGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16778..18049
                     /gene="sufD"
                     /locus_tag="ACHJ8C_002952"
     CDS             16778..18049
                     /gene="sufD"
                     /locus_tag="ACHJ8C_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO5552775.1"
                     /translation="MAGLPNSSNALQQWNHLFEAEGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLDGLTNSQFVSIAGEIAPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTVRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            18046..19266
                     /gene="sufS"
                     /locus_tag="ACHJ8C_002953"
     CDS             18046..19266
                     /gene="sufS"
                     /locus_tag="ACHJ8C_002953"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO5552776.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLEMLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19279..19695
                     /gene="sufE"
                     /locus_tag="ACHJ8C_002954"
     CDS             19279..19695
                     /gene="sufE"
                     /locus_tag="ACHJ8C_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO5552777.1"
                     /translation="MAVLPDKEKLLRNFLRCTNWEEKYLYIIELGQRLPELRDEDKSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            19844..20848
                     /gene="ldtE"
                     /locus_tag="ACHJ8C_002955"
     CDS             19844..20848
                     /gene="ldtE"
                     /locus_tag="ACHJ8C_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO5552778.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(20911..21147)
                     /gene="lpp"
                     /locus_tag="ACHJ8C_002956"
     CDS             complement(20911..21147)
                     /gene="lpp"
                     /locus_tag="ACHJ8C_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO5552779.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         5530 a         5148 c         5355 g         5171 t
ORIGIN      
        1 tctgaataat aactatcgcg aaaaaatcac tttagagcag ttatcgaaaa aatttcgtgc
       61 cagcgtcagt tatatctgcc atgaatttac caaagagtat cgtatttccc ctattaacta
      121 tgttattcaa cggcgtatga cggaagcgaa atggtcactc actaatactg aattatcaca
      181 ggcagagatt tcctggcgtg tgggttatga aaatgtcgat cactttgcca aactgttttt
      241 gcgccatgtc ggctgttcgc ccagcgatta ccgcaggcaa tttaaaaact gttttgcgga
      301 acaagaaatc ctatctgaat ttcctcaacc ggtaagtctt gccggataaa tctggcggcg
      361 cagtttgtta agaagctgcg ccgcctgcag attatttgtt ctgataatct ttcaggatct
      421 gccgacctgc tacgtaaatc ataatttcgt ctgtaccgcc gccgatacgt tcacaacgga
      481 catcacgcca gaagcgggag acgcgcgcct catcggtata gcccagaccg cccatgattt
      541 gaatcgcatc atcaatgact tccattgcgg tacgtgcgca gtacagtttt gccagcgccg
      601 cgctggtgcg cagtgactga tgctgatcgg cttgccatgc cactttcaac accatgttgc
      661 gcatgttgtc gatcttaatc gccatcagcg ccagtttttc ctggatcatc tggttatgac
      721 caatgggctt accaaaagcg atacgctggt tggcatagcg ggcggcatct tcgaatgcgc
      781 attcggcaaa gccggtgctg cgcgcggcgt tgatcaggcg ctccatctca aagttgtaca
      841 tcacattgag gaaacccatt ccttcttcgc ccaccatgtc gctctcttca acttccacgt
      901 tgtcgagata gacttcgcag gtgctgagca tatgccagcc gattttatgc agtggattga
      961 ttttaatgcc gggtttactg gagtcgaccc accacagagt aaaggccttt ttcggatctt
     1021 tcggttgcgg atcgcgcgcc aacaccagca tatatggata ctctttcgca ccggtaataa
     1081 aggttttctg tccgttgatg taaaccttgc cgtttttacg cgtgtaagtg gtagtggcac
     1141 tgttgttgtc tgagcctgcg cctggctccg tcaatgccag ggcataggcg ggatcacccg
     1201 tttccagggt gctctctgcc gttttacgta gctgctctgc agaaccgaaa cggcgcatac
     1261 tgtgaataca ttgaccgttg gtaatcaaaa atgccggagc accgcatttt gacacttcca
     1321 tcagcgccag catttgggtg acgtaatccg cagggatacc accaaattct tccggcacgc
     1381 caagcatgga aataccgtta tccgccagtg cccgcataaa ctcacgcggg tatgtcccgt
     1441 tttgatcgca ggtgcggaaa tactcttccg gaaagttagt cgtaatcagt tcgcgaatac
     1501 tggccagcag cagttcttgt tcttcagtta aagaaaaatc catcattata ctcctgttaa
     1561 tgagccgctt caggcaggac aaaacccatc gccgcatcga taaataatct ttcgtccatc
     1621 agtttgagtt ctggcgaaat cactggggtg aaatccattt tgtcgagaat atctttttgt
     1681 aaatcgacgc caggagcgat ttcgattaaa tgcaggccgt cttcttttaa cgtgaatacg
     1741 gcgcgctcag tgatataacg aacatccagc cctcgctcga gagcgatttt tccgctgaaa
     1801 gttatttccg gtagttcccg aataaatttc ttcacccgtc cttcctggac gatatttaat
     1861 ttgccgtcgg taatttctgt ttttaaactg cccgcagtta atgtgccgca gaaaacgatt
     1921 ttcttcgaag tggcactgat atcaataaat ccaccggtgc ccatgatttt accattgaat
     1981 ttatgcacgc cgacgttacc gtgctggtcg acttcagcaa aactcaaata acaaacatcc
     2041 agaccaccac cgtgataaaa atcaaactgg gacgtcatat ccagaatggc gcgggtattc
     2101 acgttcgcgc caaaggcgat cccctgtgaa gtaataccgc caatcggacc tgtttctacc
     2161 gtcagaataa agtcatcagc acaaccttct tcccgggcga ccaggccaat gccgtcagca
     2221 ataccgacgc cgacattccc cactgcgcct ttgcgcattt cgaataatgc acgccgtgca
     2281 actaatttac gttggtttag gggcaggcta agtttggtgc tgtcatcaag agtgaagtca
     2341 ccagaaataa agcggttaac cggcgcaccg ccatacagtt gggtttgatc cggatcgacc
     2401 accacaatat ccaccagata acccggaata cggacagatt taggatgcag cgtggctttc
     2461 ttaaccattt tctgcacctg catcatcaca ataccgccgt tattgtgcac cgcctgggca
     2521 ataaccaatg cgtcgagata catcacctca tcttcaaaag tggcgtagcc ttcgctgtcg
     2581 caggtggtag cgcgaatgaa ggcgatatct ggcgcaatcg ctttgtaata gagatattct
     2641 ttgttatcaa actcgaccag tttaatcagg tcttctttag tgacttcatt cagtttgccg
     2701 ccttgctggc gtggatcgac aaatgttccg atgccaatat cactaataat accaggctgg
     2761 tgggctgcgg cggcgcgtaa ggtttgtgta agtacacctt gtggatagtt ataagcaata
     2821 attttatttt gttctgcgag atcagaaata cgcggcgatt gtccccagtg accgcataat
     2881 gcccatttca ccagaccttc ttgtgccagc ggactaatac cacggtcggc gcgatcgcca
     2941 agccctgttg gactaataat cgataaatta cgtggtgttt gagtctgttt atatttatca
     3001 gcaagagcag taattaacgt ggtggcttcc agaataccgc cgccagcgcc taacacacaa
     3061 agtgttgctt cgtcgggaat atagttcacc gcttcctgtg ccgacaggac cggcactcgt
     3121 ccattaatac gaggtggttt tacaggtttc attgttaatt cctgattcag gtgtgatttt
     3181 ccctacgcca gatggagaat tagcgcacag agactcacga aatattttta ttaaatacgg
     3241 ttaaaaatac tttttttagt tcggcgggag agggtattcc cgccgaaaat attattgctt
     3301 atgcctgatg taaaatagtt aataccgtac gcaaatcatt taccgagatt tgccctggcg
     3361 cagaggcttt ttttaccgca ccaaaagttg ccgccgaccc aaatacttca ccagccagac
     3421 gagaaattac gccagttttt gccatcgaca tcgtgatgat tggacgatcg gcatactgtt
     3481 cctgcatctc cagggtcgcg gcaagcaacg tcagcacatc gctggtactt tgcggcatca
     3541 gcgcaatctt aggaatatcg gcgtcgaagg actgcatttt gcgcagacgg gcaatgattt
     3601 cttcggcttc cggcgtttta tggaagtcat ggttggacat gacaactttc acatcatgcg
     3661 cgtgggcgta ggcgacggtt tctttaacct gatcatcgcc ggtaaataac tccagatcga
     3721 tcatatcaac caggccactg tcgatggctg cacgattgag tgcaatataa gcctcggtgg
     3781 aaatcgcctg ctcgccgcct tctttggcac tgcggaaggt aaacagcagc ggtttttctg
     3841 gcatggtttc acggagaatt tttgctgccg ctatgacaga ctccacattg gagagatcgg
     3901 catagtggtc cacacgccat tccagaatat caaagtccgc ttcacgatag gcgagagctt
     3961 cggatttcac gctggcgata tctttcgcca tcagcgagac gatgatttta ggtgcgcccg
     4021 taccaatgac gagatctttt acagttacgg ttttcatttt ttaccctttc tgcacgcggt
     4081 cagcctgtca ggcaccgaac cccatgacct gtttaacata ttccagaggg aaatctttgc
     4141 cagtccataa cgtgaactgt tcagcccctt gccacaacaa catgccgtat ccatcaatcg
     4201 ttttgcaacc cgcttgttgc gcctgctgca ataacttcgt catatgcggg ttatacacgc
     4261 attcagtgac cagaagtccc ggatgtaaca gactgatatc attaaccaat gattcattct
     4321 caaggggttt catacccact ttggtgccat tggttaaaat gtcggcggaa gccagggctt
     4381 cagcaaaggc ttgctgatcg gcgagatcgg tgactgtcac gacgcaatcg gtgttttcgt
     4441 taacccgctg cgcgaaggcg agggctttat cgaagaactc atcccgacgg ttaaagagtt
     4501 taatttcttt taaaccttca attgccccct gcgcgccaat tgccgttgag gcacctccgg
     4561 cccctaacag caccatcgtt ttgcctttga tatcaaaacc gctctcttta atggcgcgaa
     4621 tatggcccgt gccgtcggtg ttatagccac gcagatagcc atcatcattt acgatggtgt
     4681 tgatggcacc caccagtttg gctgctggcg ttaattcatc aacatattca cacgccagtt
     4741 gtttgttcgg cattgataca ccagttccgc gcattttgag ggcttttaat ccttcaattg
     4801 ctccgggaaa actatcgtta tccacttcaa aggccatata ggtaaatggc aatcccgctt
     4861 tttctaaggc tttattctgc atttcgggcg ataaactgtg gcggatggga taggccatca
     4921 acccaatcaa ttcgtatttt gcggtaacat ccataattaa ctcctttaac ctctatgctt
     4981 aatcgtgttt aaccgggtgc taaatttatg ctcgccaaac cgcacatcat tttcaggaat
     5041 aatgaaaacg cggtaatagc ggataaaaac aataattgcg gtaatcaggg ccagcagcgc
     5101 gaaagtaaaa tcgagaacaa tgatatattg caggccgatg ttcgacagat aaccggtaat
     5161 cagcggaata acaaagttag ccagtccacc catcatcata tatatactgg tgactttggc
     5221 tttgcttttt gggaaaaact ctgacatgac cgaaacgccg agctgtaaaa tgccgccagc
     5281 tgctgagaaa ccgataacaa aggctccggc attacacacc aatggagaag ggtacaggta
     5341 gataatggca gctgttattg ttgccagtgc agagttaaat acattagccc agatgggccg
     5401 gaccattttt ttcagcaggg cggcaaaaat aaagacacag accagcgaac ccatactgta
     5461 ataagagatg gtttttaatg cctcagcttc tgacatacca gcaaaagcca tcgcatattt
     5521 gggcatccac accacaatca cataaaaggt cgagaatgcg gctacaccga acagtactga
     5581 tgaaacacct tccagccaga ctaacggttt gctgttcatt tgcggtaatt cattagctac
     5641 gctggcgtcc accaactggc tggggaattt gcttttcaac agcatcagcg tgatcagtac
     5701 aaacagaata cccggaataa tcagcccata gccgtaccag atgttattga gcaacatata
     5761 gctcaccagc attgggtaga acatttgccc gaatgacacc atcgctttaa ccagtatgac
     5821 cgccgaacca gaggctttcg gaaagcattc cataagcgcg gggtagccac ccgtatccag
     5881 cgctgagtta gcgataccta cgcacactgc cagacagtag gcgagagtta aattcgggca
     5941 agcaggaata ccaaagaaga atagcagata cattattact gccattaata tcaccgcccg
     6001 acgaccaaac ttatcggaga tcactccgaa gaataaaata ctgatcaatc gccccaatcc
     6061 gataccggaa attaaatagg caatgcccgc gttgtcagtg gaaaactttt ctgccaaaga
     6121 tgtcatgttt tgggcaagcg taataacact aatgccgtgc aggaaatagc tgaagtaaat
     6181 acaaagaaca gccaggataa atggcgtgct gaaagcctta ttttgagaca ttttcacagc
     6241 tccttgctga aagataattt cgttaccgta cgtattgttc tccgacggtt tacataatta
     6301 ctatactgaa gataattttc cctgggtaca tggcataaag tttatgtttc caggaatatt
     6361 gcatgtcata gtttgttttt gatcgcgatt ttccttagtt agcgcagaaa gagttattta
     6421 tccgtaagat cttgcataac ctgaaaagaa tattgcatta cccacccgga gcgatatttt
     6481 cctttagcgt gtgatgccgc gtttctttgc gactgcgtaa gccaataaac agtgccagta
     6541 taaagccgat agcagcgatg gcggtatcaa accacataat atcggcaata cttctttgcg
     6601 aaagatgagc cgtaatcaac ggaatggtaa atgttgcaat gctgcccgca ctgtaataga
     6661 tccccgttgc ttttccttta gcgtagggaa aacgttccgc cattaacgtc aggccaattt
     6721 gtacaacacc gcctgcggag gtaaaaccga tgacgaaagc aaatattatc accacataaa
     6781 atgtgggatg caggcagacg gtgagcaggg cgataaatga gataaaggtg tacagcatca
     6841 gtaatgttgt tgggcgaacg gtattacgaa tgagtggagc agtaataaat acacacagca
     6901 gcgaacccac ggtgtagata ctgagtaatt tgatcgacat ggtgtatgac atgccagcga
     6961 caaattgtcc gtattgtgcc agccactggc taaccagata aaacgttgcc attgagatat
     7021 agccatatag ggaataactg gctaaatcga taattgaaca gcgatgttcc gtagagctgg
     7081 tggttttctt tatgacaggt aagtgacgac ccggatgggg tgggaacgta caacggtata
     7141 aaaacagagc gttaataaac ataatgcctg cggcaatcat aaaggaccaa ccgaaccaca
     7201 gttcagccca cactaacagg ctgataatta gcggtaataa aaactgtccg ctggaaacaa
     7261 atgctttaat taaaatattg gctgtacccg gtgagcgtgg aaaagcttcc atcaaactgg
     7321 gataggtgcc tgcatcgaga aaactgtttg ccattcccgc cagaaagcca aaaacataag
     7381 cgataatgat gttattggtg tgcaggatgc caaaaaagaa ggccatatag cagcacatcc
     7441 cgagcatgat aaaagggcgg cgaccaaagc gatcggataa taatcctgca aaaagcaaga
     7501 cacttaatcg accaatgccc agtgatgaga taactatcga gacgcccgcg gcattagtct
     7561 gccaaagcgt ctccagcgag gccatattca ggctcatcaa aatgacgccc ataccatgca
     7621 ccaggtaatt aaaatacaac ccaagtgcgg tagggtaata gggatttttc ataggttcta
     7681 cttagcaaag gtgctttaaa aagtgtagcg atgttgtgtc agtatccgtt cattattgtt
     7741 gtaacaacga caattccttt ctgtggagaa attaacaaag ccgctccaga tcgtgtgcat
     7801 ataattcagc ggctaccgat tgatagatct tccagtcaat aaaattgcca tcagggtaga
     7861 cttgttgaaa cgcggttaag tcggggaaaa aacgcatggt gttgttgccg atgcggttat
     7921 ttattttctc tatgatggca tgaagatgtt tttttcgttg ctgacgcata gccaacagtt
     7981 gctcgactac acaatcagga atggcgcatt tgccattttc ccactgacgc cagctttcac
     8041 tgttaccggt ctgggcaatc caggtcgcgc attcatcaat agtcatggca aaaatatggc
     8101 gaagtgcctg gagttcataa gcgttcatcg tgtaatggct gcatgaaata aacaataaaa
     8161 tataaataac attatcagcc tgctgacggc ttgaagagag tcacagtatc ttgtgcaaca
     8221 ttattggaaa gagtgagggg cgaggtagcg aagcagaaaa atgttaaaaa aaagcccatc
     8281 gtgggagatg ggcaaagact acacacagca attcgttgtt tcactcaggg gatttccatg
     8341 cttataaatc aatatgttga tttataaccg tgagctaata gtaggcatac ggactttttg
     8401 cgtcgatcag attcgtctca ttagtttaaa aactgtaaag aatttgcgac aggtactacc
     8461 agctgaatag cggtaagcag gaaaatatgg ggaagagcaa gcaaatatca attagcaggg
     8521 gttaacaatc aggcggcaga gccgcctgat gagaaattac ttattcgatt tctcgatttc
     8581 gccaagttct tcgagaattt cttccggttg gtccgtcggc ggtgggactt cttccaccca
     8641 cgcggcaaac agacgccagg aaacggctaa cagaaccgga ccaataaaca gaccgatcat
     8701 cccgaaagca atcaaaccac caataacgcc agagaggatc aggatcagcg gtaaatcggc
     8761 acccatgcga attaacattg ggcggatgac gttatcgagc gtgccaacca caccgctcca
     8821 aactaacaat accgttcccc aggtggtatc gccagtccag tagagccaga taatcgccgg
     8881 aatcagtacc ggcaacggtc caagctggac aaggcaggag aggatcatta acaccgttag
     8941 caaagttgca taaggtacgc cggatacggc gaggccgata ccgccaagca ctgcctgtac
     9001 taacgccgtc accaccacac ccagcgccac cgcgcggata gcctgtgccg ccagcagcac
     9061 ggcggcatct ccgcgaacgc ctgccagacg ggttgcaaaa tggcgaatgc cttgtgccac
     9121 ctgttcaccg cgccagtaca gcagggcgct gaagagaagc atcagcgcac aatgcaccat
     9181 aaagcgcccg atatgcgccg cctgcccaac gaaccaggtg gtggtggtgc caatataagg
     9241 acggactttc gccatgatcg ccgtcccccc catatccagc aaattgtgcc agcctgcata
     9301 tagcttcgcg ccaatcaccg gaatggtatt aagccacgcc agatcgggta acgtcatgtc
     9361 accactggaa atggctttaa ttagcgggcc gctgccgtcg acgatactgt taaccagcaa
     9421 agcaataggg atgataaaca ccatcactaa taacagcgtc atcaccagaa cggcgagaga
     9481 gcggcgacca aacatgatct tttgcaaacg taacaatacc ggccaggtgg cgataaccac
     9541 tgtaccggcc catgcaaagc cgagaataaa gggttgtaca atccacagac atgccacaat
     9601 catgatggct aaaaacagca ccgaaagcag aatttgtgcg acatccctgg gctgacgaac
     9661 atttaccata actacttttc accttttttg tgcgtcaaat cgtcgacgcg acgtgaacac
     9721 gcgaaactca cctgcataat gatgagcaat ttcagcggtt tttaacaggt cgattctgcc
     9781 cttaattctg atgggcgtgt aagaaaaaaa tgtggtacaa caactcaggc aacacgcaaa
     9841 cgattaatct ctcatcacag accgcaggaa agggtcaata taatgattcc acagatttcc
     9901 caggcacccg gcgtcgttca actggtgctt aattttttgc aagagctgga gcaacagggt
     9961 tttaccggcg atacggcgac aagttatgcc gatcgtctga caatgtcgac tgacaacagt
    10021 atttaccaac ttctccccga tgcggtggta tttccgcgtt caaccgcaga tgtagcgctg
    10081 atcgcccgtc ttgccgcgca ggaacgctat tcatcgttga tctttacccc ccgcggcggc
    10141 ggcaccggca ctaacggaca ggcgctcaac caggggatta ttgttgatat gtcccgccat
    10201 atgaaccgca tcatcgaaat taaccctgaa gagggctggg tgcgcgttga ggccggggtg
    10261 attaaagacc aactcaatca gtacctgaaa ccctttggct acttttttgc gccagaactt
    10321 tcgaccagta accgggcaac gctcggcggg atgatcaata ccgatgcatc cggtcaggga
    10381 tcgctggtct atggcaaaac gtcagatcac gtacttggcg tacgcgcggt gttgttgggg
    10441 ggcgatattc tcgatacgca acctttaccc gtcgaactgg cggaaacgct gggtaaatcc
    10501 aataccacaa tcgggcgaat ttataacacg gtttatcaac gttgccgtca gcaacgccag
    10561 ttaatcatcg acaacttccc caagcttaac cgctttctta ccggttacga tctgcgtcat
    10621 gtctttaacg atgagatgac cgagttcgac ctgacgcgca ttctgacggg ttcagaaggg
    10681 acgctggcct ttattaccga agcgcgactg gatattacgc gcttgcctaa agtacgccgt
    10741 ctggtgaacg tcaaatatga ctcttttgac tccgcgctgc gtaacgcgcc gtttatggtt
    10801 gaggcgcggg cgctttcggt agagacggtg gactcaaaag tgctgaatct ggcgcgggaa
    10861 gatattgtct ggcattccgt cagcgagttg attaccgatg tgcctgataa agagatgctc
    10921 gggctgaaca ttgtggaatt tgccggtgat gatgaggcac tgattgacga gcgagttaat
    10981 gcactctgtg tgcggcttga tgagctgatt gccagccaac aggcaggagt gatcggctgg
    11041 caggtgtgcc gcgagctggc gggtgttgaa cgtatctatg cgatgcgcaa aaaagccgtt
    11101 ggtctgcttg gcaatgccaa aggtgctgct aagccaattc cgtttgctga ggatacctgc
    11161 gtaccgccgg aacacctggc ggattatatt gctgaatttc gcgcgctgct cgacagccac
    11221 ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg tcttgcacgt ccgtccggca
    11281 ctggatatgt gcgatccgca acaagagatt ttgatgaagc aaatctctga tgatgtagtg
    11341 gcgcttaccg cgaaatacgg tggtttgttg tggggcgaac acggcaaagg ttttcgcgcc
    11401 gaatacagcc cggcgttttt tggtgaggaa ctttttgcag aactgcgtaa agtgaaagcg
    11461 gcgtttgatc cgcataaccg actcaacccc gggaagattt gcccgccaga aggtctcgat
    11521 gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat tcgatcgaca gatccccatt
    11581 gcggtacgcc agcagtggcg tggtgcaatg gagtgtaacg gcaacggttt atgcttcaac
    11641 tttgatgctc gtagtccgat gtgcccgtcg atgaagatca cccagaaccg gattcattca
    11701 ccgaaagggc gcgcaacgct ggtacgtgaa tggctgcgtt tgttggcgga tcgcggcgtt
    11761 gacccactca aactggaaca agaactgcct gaatccggcg tcagtttgcg gacgttaatt
    11821 gcccgcacgc gcaatagttg gcatgcgaat aaaggcgaat atgacttctc acacgaagtc
    11881 aaagaggcga tgtcgggctg tctggcctgt aaagcttgtt cgactcagtg ccccatcaaa
    11941 attgatgtgc cggagtttcg ctctcgtttc ctgcaactct atcacacccg ttatttacgc
    12001 ccgctgcgcg accacctcgt cgctacggtt gagagttacg caccgctgat ggcacgcgcg
    12061 ccgaaaacct ttaacttctt cattaaccag ccgctggtgc gcaaactctc ggaaaaacat
    12121 atcggcatgg ttgatttgcc gctgctgtcg gtcccctcgc tacaacaaca aatggtgggg
    12181 catcgctcgg caaacatgac gctggaacag cttgaagctc tcaatgcaga gcagaaagcg
    12241 cgcacggtgc tggtggtgca ggaccccttt accagctatt acgatgctca agtggtggcg
    12301 gattttgtcc gtctggtcga aaaattaggt ttccagcccg tgttactgcc attttcgcca
    12361 aatggaaaag cccagcatat caaaggtttt cttaatcgtt ttgcgaagac ggcgaaaaag
    12421 acggcggatt tcctcaaccg tatggcgaag ctgggtatgc caatggtagg cgtcgacccg
    12481 gcgctggtac tttgttatcg cgatgaatat aaactggctc tgggcgagga gcgtggcgag
    12541 tttaacgtct tactggcaaa tgaatggctg gcaagcgcac ttgagtcaca gccagtgact
    12601 acagtcagcg gtgaatcatg gtatttcttt ggtcactgta ccgaagttac cgccttgccg
    12661 ggcgcgccag cacaatgggc ggcaatattt gcccgttttg gcgcgaaact ggaaaatgtc
    12721 agcgtggggt gctgcggcat ggcagggact tacggacatg aagcgaaaaa ccataaaaat
    12781 tcgctcggga tctatgaatt atcctggcat caggctatgc agcgactgcc gcgtaaccgc
    12841 tgtctggcga ccggatattc ctgccgtagt caggtaaaac gggttgaagg cacaggggta
    12901 cgccatcctg tgcaggcttt actggagatt attaaatgat atggaaacga aaaatcaccc
    12961 tggaagcact gaatgctatg ggggaaggaa acatggtggg attgctggat attcgctttg
    13021 aacatattgg tgatgacacc cttgaagcga caatgccagt agactcacgg acaaagcagc
    13081 ctttcgggtt gctgcatgga ggtgcatccg tggtactggc cgaaagtatc ggttccgttg
    13141 ccggttattt atgtacagaa ggtgagcaaa aagtggttgg tctggaaatc aatgctaacc
    13201 acgtccgctc ggcacgagaa gggcgggtgc gcggcgtatg caaaccgttg catctcggtt
    13261 cgcgtcacca ggtctggcag attgaaatct tcgatgagaa agggcgtttg tgctgttcgt
    13321 cacgattgac gaccgctatt ttgtgacgtt gtcaccagaa aagtgtgacg catattgctt
    13381 ttggttatta tctgcacagt aaacggtggt atactgtgca ggtcttgtct aaaagcgagg
    13441 atgctatgtc tactcaactt gatcccaccc aactggccat tgaattttta cgccgtgacc
    13501 aaagcaatct ttcacctgcc caatatctca agcgtctgaa acaattagaa cttgaatttg
    13561 ctgacctgct tactctctct tctgctgaat taaaagaaga aatctacttc gcctggcgtc
    13621 tgggcgtgca ttgatcctga tttaatgtcg cgagttcgcc aaatcggcct ggattaatct
    13681 tcgtccaggt tcgaaggcct ttttaataca gcctatctac ccgcctgata acgttcaaaa
    13741 ataatacaaa tcgcagtttg tgttaaaaca gtgggttagc tttatgagat tatccttatc
    13801 gccccttcaa gagctaagcc actgtgagtg ccggagataa gcgccggatg gggtagaaac
    13861 ccttaagcct gtgtcgcaca gacttaaggg ttttcttatt tctgaacgct gctctttttt
    13921 acctatccct taaatagtca ttttcaatgc aactttttct ttttcatcac agccttaatg
    13981 ctccttagat aataatcatt atctaacaat gagataccta attcttagcg tgcctgttaa
    14041 cccacaaatc agggtctatg cttattaaaa gcaggtaaaa agcatgtttt acgctaaagc
    14101 ccctgcgttt gctgggttga actgataatc attatcacta acatgctgtt atacgctgaa
    14161 agcgatgaag tgaggtaaat cgatggacat gcattcagga acctttaatc cacaggattt
    14221 cgcctggcaa ggcttaacgc tgacacccgc agcggcgata cacatccgcg aactggtggc
    14281 aaagcagcct ggtatgatcg gcgtgcgctt aggcgtgaag caaacaggct gcgcgggctt
    14341 tggctatgtg ctcgacagtg ttagcgagcc ggacaaagac gatctgctgt ttgaacacga
    14401 cggcgcgaag ctgtttgtcc cgctgcaagc gatgcctttt attgatggca cggaagtcga
    14461 tttcgttcgc gaaggactta atcagatatt caaatttcac aaccctaaag cccagaatga
    14521 atgtggctgt ggcgaaagct ttggggtata ggcggtacta tgtctcgtaa tactgaagca
    14581 actgacgatg tcaaaacctg gaccggcggc ccgctgaatt ataaagaagg attcttcacc
    14641 cagttagcca ccgatgagct ggcaaagggg ataaacgaag aggtggtgcg cgcaatttcg
    14701 gcgaagcgta atgagccgga gtggatgctg gagtttcgtc tcaacgccta tcacgcatgg
    14761 ctggagatgg aagaaccgca ctggttgaaa gcgcactacg acaagctgaa ttatcaggat
    14821 tacagctact actcggcacc atcgtgcggt aattgtgacg acaactgcgc gtctgaacct
    14881 ggcgcggtgc agcaaactgg cgcgaacgcc tttttaagta aagaggtgga ggcggcgttt
    14941 gagcagttgg gcgttcccct gcgggaaggc aaagaggtgg cagtggatgc cattttcgac
    15001 tctgtttcgg ttgccactac ttatcgcaaa aaactggcgg agcagggaat tattttttgt
    15061 tcctttggcg aggcgatcca cgatcacccg gaactggtgc gtaaatatct cggcaccgtg
    15121 gtgccgggga atgacaactt ctttgccgca cttaatgcgg cggtagcctc tgacggtacg
    15181 tttatttatg tgcctaaagg cgtgcgttgc ccgatggaac tttccaccta ttttcgcatt
    15241 aacgcggaaa aaaccgggca gtttgagcgc accattctgg tggccgacga agacagctac
    15301 gttagctaca ttgaaggctg ttccgctccg gtgcgtgaca gctatcagtt acacgcggcg
    15361 gtggtggaag tcatcatcca taaaaacgcc gaggtgaaat attccacggt acaaaactgg
    15421 ttccctggcg ataacaacac cggcggtatt ctcaacttcg tcaccaagcg tgctttgtgc
    15481 gaaggcgaaa acagcaaaat gtcatggacg caatcagaaa ccgggtcagc gattacgtgg
    15541 aaatatccca gctgcatttt gcgcggcgat aactccattg gtgagtttta ctcagtggcg
    15601 ctgaccagcg gtcatcagca agcggatacc ggcaccaaga tgatccacat cggtaaaaac
    15661 accaaatcga ccattatctc gaaagggatc tctgccggac atagtcagaa cagttatcgc
    15721 ggcttagtga aaatcatgcc gacggcaacc aatgcgcgca atttcactca gtgcgattca
    15781 atgctgattg gcgctaattg tggggcgcat accttcccgt atgtcgaatg tcgcaataac
    15841 agcgctcagc tggagcacga ggcaacgaca tcacgtattg gtgaagatca actgttttac
    15901 tgcctgcaac gcgggatcag cgaagaagat gccatctcga tgattgttaa cggtttttgc
    15961 aaagacgtgt tctcggaact gccgctggaa tttgccgttg aagcacaaaa actcctcgcc
    16021 atcagtcttg aacacagcgt cggataagga ataaacatgt taagtattaa agatttacac
    16081 gtcagcgtgg aagataaagc tatcctgcgt ggattaagcc tcgacgttcg tcccggcgaa
    16141 gttcacgcca ttatggggcc caatggttcg ggcaaaagta ccttatcggc aacgcttgcc
    16201 gggcgagaag attatgaagt gacgagcggc acggttgagt ttaaaggcaa agatttgctt
    16261 gcgctgtcgc cggaagatcg tgcaggtgaa ggcatcttta tggccttcca gtatccggtg
    16321 gaaattcccg gcgtcagtaa ccagtttttc ctgcaaacgg cacttaatgc ggtgcgcagc
    16381 tatcgcggcc aggaaacgct cgaccgcttt gattttcagg atttgatgga agagaaaatc
    16441 gctctcctga agatgccgga agatttatta acccgttcgg taaacgttgg tttttccggc
    16501 ggcgagaaaa agcgcaacga tattttgcaa atggcggtgc tggaaccgga gttatgcatt
    16561 cttgatgagt cagactccgg gctggatatt gacgcattaa aagtggttgc cgatggcgtg
    16621 aactcgctgc gtgacggcaa gcgttcattc atcattgtta cgcactacca acgcattctc
    16681 gactacatca agcctgatta cgtccatgtg ctgtatcagg gacgaattgt gaaatccggc
    16741 gatttcacgt tggtcaaaca actggaggag cagggttatg gctggcttac cgaacagcag
    16801 taacgcactg caacagtgga atcatttgtt tgaagctgaa ggggcgaaac gctccccgca
    16861 agcacagcag catttacaac aattgctgcg taccggactg ccgacacgta aacatgaaaa
    16921 ctggaaatat acgccgctgg acgggctgac caatagccag tttgtcagca ttgcggggga
    16981 gatagcccca cagcagcgtg atgccttagc gttaacgtta gacgccgtgc ggctggtgtt
    17041 tgtcgatgga cgttacgtgc ccgcactgag cgatgcaact gaaggcagcg gatatgaagt
    17101 gagcattaac gacgaccgtc aggggttacc cgacgctatt caggcggaag tgtttctgca
    17161 tttgacggaa agcctggcac aaagcgtgac gcatatcgcc gtgaagcgcg gtcaacggcc
    17221 ggcaaagcca ttgctgttaa tgcatatcac ccagggcgtg gcaggtgaag aggtgaatac
    17281 tgcccattac cggcatcatc tggatctggc ggaaggtgcc gaagcaacgg tgatcgaaca
    17341 ttttgtcagc cttaatgatg ctcgtcactt caccggcgca cggttcacta tcaacgtcgc
    17401 agcgaacgcc catttgcagc atatcaagct ggcgtttgaa aacccgctca gtcaccactt
    17461 tgctcataac gatttgttgc tggctgatga tgccaccgca tttagccaca gtttcctgct
    17521 gggtggcgca gtgttacgac acaacaccag tacgcaactc aatggcgaaa acagcacggt
    17581 gcggatcaat agcctggcga tgccggtgaa aaacgaggtg tgtgatactc gtacctggct
    17641 ggaacacaat aaaggttttt gtaacagccg acagttgcac aaaactatcg tcagcgacaa
    17701 aggccgcgcg gtatttaacg gtttgatcaa cgtcgcgcag cacgccatca aaacggatgg
    17761 tcagatgacc aacaataatc tgctgatggg caaactggcg gaagtggata ctaaaccgca
    17821 gctggaaatc tatgcagatg atgtgaaatg cagccacggc gcgacggtgg ggcgtattga
    17881 tgatgaacag atgttctatc tgcgctcgcg cgggatcaat cagcaggatg cccagcagat
    17941 gatcatttac gcctttgctg ccgaactgac ggaagcactg cgtgatgaag ggcttaaaca
    18001 gcaggtgctg gcccgaatcg gtcaacggct gccaggaggt gcaagatgac tttttccgtt
    18061 gataaggtgc gggccgattt cccggtgctt tcgcgtgagg tgaacggttt gccgctggct
    18121 tatctcgaca gcgccgccag tgcgcagaaa ccgagccagg tgattgatgc cgaggctgag
    18181 ttttatcgtc atggctacgc ggcggtgcat cgcggtattc ataccttaag cgcccaggcg
    18241 accgagaaaa tggagaacgt gcgcaagcgg gcatcgctgt ttattaatgc ccgttcggcg
    18301 gaagagctgg tgtttgtccg cggcacgacg gaagggatca atctggtcgc caatagctgg
    18361 ggcaacagca atgtgcgggc gggcgataac atcatcatca gtcagatgga gcaccacgct
    18421 aacattgtcc cctggcagat gctttgcgca cgcgttggcg cagagctgcg tgtgatcccg
    18481 ctcaaccccg acggcacgct gcaactggag atgctaccta atctgttcga tgagaaaact
    18541 cgcctgctgg caattactca tgtctccaac gtgctgggaa cggaaaatcc gctggcggaa
    18601 atgatcacgc ttgcgcacca gcatggcgca aaagtgctgg tggatggcgc tcaggcggtg
    18661 atgcatcatc cggtggatgt tcaggcgctg gattgcgatt tttacgtgtt ttccgggcat
    18721 aaactgtatg gccccaccgg gattggcatt ctttatgtca aagaagcctt gttgcaggag
    18781 atgccgccgt gggaaggggg cggttctatg atcgccaccg tcagcctgag tgaaggcact
    18841 acctggacca aagcaccttg gcggtttgaa gccggtacac ccaataccgg gggcatcatt
    18901 ggtcttggcg cggcgctgga gtatgtttcg gcgctggggc ttaataacat agccgagtat
    18961 gaacagaatc tgatgcacta cgcgctatca cagctggaat ctgtaccgga tctcactctc
    19021 tatggcccgc aaaacaggct tggcgttatt gcttttaatc tcggtaaaca ccacgcctat
    19081 gatgttggca gttttctcga taattacggc attgctgtgc gtaccggaca tcactgcgca
    19141 atgccattaa tggcctatta caacgtccct gcgatgtgtc gggcatcgct ggccatgtat
    19201 aacacccatg aagaagtgga tcgtctggta actggattgc aacgtattca tcgtctactg
    19261 gggtaaaagg gaggctctat ggctgtattg ccggataaag aaaaattgct gcgtaacttt
    19321 ttacgatgca ccaactggga agagaaatat ctctacatta ttgaactggg acagcgtctg
    19381 ccagaattac gtgacgaaga caaaagcccg caaaatagca tccagggctg ccagagtcag
    19441 gtgtggattg tgatgcgtca aaatgcgcag ggaattattg aattacaggg cgacagcgat
    19501 gcggccattg taaaagggct tattgcggtc gtctttattc tttacgatca aatgacgccg
    19561 caggatattg tcaatttcga tgtgcgcccg tggtttgaaa aaatggcgct cacccaacat
    19621 ctcaccccat cacgctcaca aggtctggaa gcgatgattc gcgcaattcg cgccaaagcc
    19681 gctgcactta gctaaactaa agggacagct ttcatccggt tggctcacat tgccgggtgg
    19741 tcaatgttta cactggcctg cacaggagtg ttttggtttc aggtgaacat aagcgccatc
    19801 cgacgtttca gcgtgagtct ccggcaaata caggaggttt acaatgaaac gcgcgtcttt
    19861 gcttacactc acgcttatcg gcgcttttag cgccatccag gctgcctggg cggttgatta
    19921 tccgctacca ccaaccggca gtcgactggt tgggcaaaat caaacgtata cggtgcaaga
    19981 aggggataaa aaccttcagg ccatcgcccg acgttttgat actgcggcaa tgttgatcct
    20041 tgaagccaat aacactatcg ccccggtgcc aaaacccggt acgacgataa ctattccttc
    20101 acaactatta ttacctgatg cgccgcgtca ggggattatt gtaaaccttg cagagctgcg
    20161 cctttattat tatccgccgg gagaaaatat cgtgcaggtt tatccgatag gtattggttt
    20221 gcaggggctg gaaacgccgg tgatggaaac gcgtgtgggg cagaaaatcc ctaacccaac
    20281 ctggacgcct acggcgggca ttcgtcaacg ttcgctggag cgtggcatta aattaccacc
    20341 agtcgttcct gccgggccca ataacccact aggacgttac gcactgcgcc tcgcgcatgg
    20401 taatggcgaa tacctcattc atggcaccag tgcgccggac agcgtcggtt tgcgcgtcag
    20461 ttcgggctgt attcgcatga atgcaccgga tattaaagct ttattctcca gcgtgcggac
    20521 gggaacaccg gtgaaagtga tcaacgaacc ggtgaaatat tccgtggaac ctaacgggat
    20581 gcgttatgtt gaagtacatc gaccactatc ggcagaagaa cagcagaacg ttcagacaat
    20641 gccgtacacg ctgccagcag gctttacgca atttaaagac aataaggctg tagatcagaa
    20701 gttagtcgat aaagcgttgt atcgtcgggc agggtatccg gttgcggtga gcagtggagc
    20761 aactcccgca gccagcaatg cgccttcagt agagtcagcg cagaatgggg aaccagagca
    20821 agggaatatg ttacgcgcga cgcagtagct gtaaatagca ggcaaaaaaa tggcgcacaa
    20881 tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag ccatgttgtc
    20941 cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg aacgcattgc
    21001 gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt cagaagacag
    21061 ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca ggattaccgc
    21121 gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt taatctccat
    21181 gtagcgttac aagtattaca caaa
//
LOCUS       DAZFJL010000082        21089 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFJL010000082 DAZFJL010000000
VERSION     DAZFJL010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.082"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            130..462
                     /gene="hdeA"
                     /locus_tag="ACHJ8C_002957"
     CDS             130..462
                     /gene="hdeA"
                     /locus_tag="ACHJ8C_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO5552780.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKKASFKDKVKGE
                     WDKIKKDM"
     gene            578..904
                     /gene="hdeB"
                     /locus_tag="ACHJ8C_002958"
     CDS             578..904
                     /gene="hdeB"
                     /locus_tag="ACHJ8C_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO5552781.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            968..1615
                     /gene="yhiD"
                     /locus_tag="ACHJ8C_002959"
     CDS             968..1615
                     /gene="yhiD"
                     /locus_tag="ACHJ8C_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO5552782.1"
                     /translation="MTAEFIIRLILAVIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLGHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1667..2437)
                     /locus_tag="ACHJ8C_002960"
     CDS             complement(1667..2437)
                     /locus_tag="ACHJ8C_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFO5552783.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(2434..3426)
                     /gene="shuU"
                     /locus_tag="ACHJ8C_002961"
                     /gene_synonym="chuU"
     CDS             complement(2434..3426)
                     /gene="shuU"
                     /locus_tag="ACHJ8C_002961"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFO5552784.1"
                     /translation="MLKDSLSSARIFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(3475..4098)
                     /gene="chuY"
                     /locus_tag="ACHJ8C_002962"
     CDS             complement(3475..4098)
                     /gene="chuY"
                     /locus_tag="ACHJ8C_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFO5552785.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGITRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVNRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(4098..4592)
                     /gene="hutX"
                     /locus_tag="ACHJ8C_002963"
     CDS             complement(4098..4592)
                     /gene="hutX"
                     /locus_tag="ACHJ8C_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFO5552786.1"
                     /translation="MSHVSLQEFLKTEPDGTLEAIAEQYNTTLLEVVKNLPSPTVVSG
                     DKFDTVWDTVCEWGNVTTLVHTTDVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            complement(4605..5942)
                     /gene="hutW"
                     /locus_tag="ACHJ8C_002964"
     CDS             complement(4605..5942)
                     /gene="hutW"
                     /locus_tag="ACHJ8C_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFO5552787.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGIETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIALEKP"
     gene            complement(5962..6876)
                     /gene="chuT"
                     /locus_tag="ACHJ8C_002965"
     CDS             complement(5962..6876)
                     /gene="chuT"
                     /locus_tag="ACHJ8C_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ChuT"
                     /protein_id="HFO5552788.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTMVKRKKLFTALLALSWT
                     FSVTAAERIVVAGGSLTELIYAMGAGKRVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            7560..9542
                     /gene="chuA"
                     /locus_tag="ACHJ8C_002966"
                     /gene_synonym="shuA"
     CDS             7560..9542
                     /gene="chuA"
                     /locus_tag="ACHJ8C_002966"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFO5552789.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            9591..10619
                     /gene="chuS"
                     /locus_tag="ACHJ8C_002967"
     CDS             9591..10619
                     /gene="chuS"
                     /locus_tag="ACHJ8C_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFO5552790.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MRGDIREILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGHVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVTA"
     gene            complement(10676..11206)
                     /gene="dctR"
                     /locus_tag="ACHJ8C_002968"
     CDS             complement(10676..11206)
                     /gene="dctR"
                     /locus_tag="ACHJ8C_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO5552791.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTIAIIDFVALI
                     NGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHINYLV"
     gene            complement(11359..11925)
                     /gene="slp"
                     /locus_tag="ACHJ8C_002969"
     CDS             complement(11359..11925)
                     /gene="slp"
                     /locus_tag="ACHJ8C_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO5552792.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(12115..12492)
                     /locus_tag="ACHJ8C_002970"
                     /pseudo
     CDS             complement(12115..12492)
                     /locus_tag="ACHJ8C_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13108..13533)
                     /gene="arsC"
                     /locus_tag="ACHJ8C_002971"
     CDS             complement(13108..13533)
                     /gene="arsC"
                     /locus_tag="ACHJ8C_002971"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO5552793.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGITVRALLRKNVEPYEKLGLAEDKFTHDQLIDFMLQHPILINRPIVVTPQG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGNRLK"
     gene            14067..14150
                     /gene="dinQ"
                     /locus_tag="ACHJ8C_002972"
     CDS             14067..14150
                     /gene="dinQ"
                     /locus_tag="ACHJ8C_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO5552794.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(14204..15556)
                     /gene="gorA"
                     /locus_tag="ACHJ8C_002973"
     CDS             complement(14204..15556)
                     /gene="gorA"
                     /locus_tag="ACHJ8C_002973"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO5552795.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(15628..16470)
                     /gene="rlmJ"
                     /locus_tag="ACHJ8C_002974"
     CDS             complement(15628..16470)
                     /gene="rlmJ"
                     /locus_tag="ACHJ8C_002974"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO5552796.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATDIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            16673..18715
                     /gene="prlC"
                     /locus_tag="ACHJ8C_002975"
     CDS             16673..18715
                     /gene="prlC"
                     /locus_tag="ACHJ8C_002975"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO5552797.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVLTLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMELFKRFRGREPQLDAMLEHYGIKG"
     gene            18723..19475
                     /gene="rsmJ"
                     /locus_tag="ACHJ8C_002976"
     CDS             18723..19475
                     /gene="rsmJ"
                     /locus_tag="ACHJ8C_002976"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO5552798.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALSD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(19524..20993)
                     /gene="dtpB"
                     /locus_tag="ACHJ8C_002977"
     CDS             complement(19524..20993)
                     /gene="dtpB"
                     /locus_tag="ACHJ8C_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO5552799.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
BASE COUNT         5376 a         5061 c         5359 g         5293 t
ORIGIN      
        1 tgtattgaaa taaaaatatc tgattttgat attttccatc aacatgacat atacagaaaa
       61 ccaggttata acctcagtgt cgaaattgat tcgtgacggc tctttcactt tatagttgag
      121 gatattacga tgaaaaaagt attaggcgtt attcttggtg gtctgcttct tctgccagta
      181 gtgagcaatg cagcggatgc acaaaaagca gctgataaca aaaaaccggt caactcctgg
      241 acctgtgaag atttcctggc tgtggacgaa tccttccagc caactgcagt tggttttgct
      301 gaagcactga acaacaaaga taaaccagaa gatgcggttt tagatgttca gggtattgca
      361 accgtaaccc cagctatcgt tcaggcttgt actcaggata aaaaagccag ctttaaagat
      421 aaagttaaag gcgaatggga caaaattaag aaagatatgt aattccggga atgcgttaca
      481 tcgtatttcc ttgcatattg aacaggccgg aatatcttct ttaaaagcag ctattcctcc
      541 tgtttgtata taatctctat attgaatggg ttacaaaatg aacatttcat ctctccgtaa
      601 agcgtttatt tttatgggcg ctgtagcggc tttgtcactg gtgaacgcac aatctgcgct
      661 ggcagccaat gaatccgcta aagatatgac ctgccaggaa tttattgatc tgaatccaaa
      721 agcaatgaca ccagttgcgt ggtggatgct gcatgaagaa acagtatata aaggtggcga
      781 taccgttact ttaaatgaaa ccgatctcac tcaaattcct aaagtgatcg aatactgcaa
      841 gaaaaacccg cagaaaaatt tgtatacctt caaaaatcaa gcatctaatg acttgccgaa
      901 ttaataaggt acaagtaaaa aggagtagca agttgagcca tcttgctgct cctttttgca
      961 tttttatatg acagcagaat ttattatacg acttatactt gcggtaattg cctgtggcgc
     1021 tattggcatg gaaaggcaaa tgcgcggcaa aggagcaggg ttacgcacac atgtattaat
     1081 tggcatggga agcgccctgt ttatgattgt ttcgaaatat ggttttgctg acgtgctgtc
     1141 tttaggtcac gtcggactgg atcccagccg tattgctgct caggttgtga cgggcgtcgg
     1201 gtttatcggt gcaggtaaca ttctcgttcg taaccaaaat attgtaggtc tgacgacggc
     1261 agcggatatc tgggtgaccg ccgccattgg tatggttatt ggcagcggta tgtacgaact
     1321 gggtatttat ggttcagtga tgaccttgct ggtgctggaa gtcttccatc aattaacctt
     1381 ccgcctgatg aacaaaaatt accatttaca gctgacatta gtgaacggta atacggtatc
     1441 aatgctcgac tggtttaagc agcaaaaaat aaaaacggat ttagtttcgt tgcaggaaaa
     1501 tgaagatcat gaagtggtcg ccattgatat ccagcttcat gccacgacgt cgatagaaga
     1561 tctgttgcga ttattaaaag gtatggcagg ggtgaaaggc gtttcaatta gctaatctgt
     1621 atagttattt attcgcagga gtaccagtgc ggtactcctg ttggttttaa tgatcgagga
     1681 cgatcagtgg tgaatggttg gcaggatctt taagtacggt aatatccgcg ccgtagagca
     1741 ttgtcagcgc ctgctgtgtc aatacgtcct gcggcttacc atttgcgata acctttcctt
     1801 tttgcattaa tacaacacgg tctgcataac gcgcggcaag atttagatcg tgcaatacgc
     1861 agcaaacatt gaactgccgt tcgtgaacta actggcgtaa taaacgaaac aagtgctgct
     1921 gatggtgaat atccagcgcc gaagttggtt catcaagaaa taaccatttc ggtgagggag
     1981 taggttccca gagttgcact agcaggcgcg ctaattgcac gcgctgttgt tcaccaccgg
     2041 ataattgccg atagtcgcgg tttgctaacg cctgacagtc acataacgcc attatttgtg
     2101 ctgtttcatt atcctgatta ccagtacgat gcggatgccg ccccatctgg ataacttctt
     2161 gtacgctgaa tggaaacgcc atgtggctat tttgacgcat cacggcccgg tgtttcgcca
     2221 gttcagtaat cgaccattca tttaatggct tattaaacaa ccgacattcg ccgctatcag
     2281 gttgaagata gccggttagc tggcgaagca gcgtagattt tcccgcacca ttagggccga
     2341 ggatggcaac aatttcccca ccgggaaaag tcagcgagac attatcagtc agacgccgcc
     2401 cctgaaggct ataaaccaga ttctgggcgc tgatcatgtg atcctccggt tgcgcagaat
     2461 caaccacata aaataagggc caccaagcag gctggttaat aatcccacgg gcatttctgc
     2521 tggctgtacc agcgtgcggg caagcgtgtc tgccatcagc aataaacagg ctccggcgag
     2581 ggcggaacaa gggattagcc agcggtgatt tgccccggta gtcatgcgaa tcagatgcgg
     2641 gatcaccagg ccaataaagc cgataatgcc gcttaccgat acggcagcac caacgagcag
     2701 tgagctcact aacagtaatt gctggcgttt acgcttcacg ttcacgccga ggtagtgggc
     2761 ttcttcatca ccgagctgca gtaaattcag cgttccggcg agacaagttg ttgcgataat
     2821 ggccggtagg atgaatgaac tggcaaccaa taacgtcggc cattgcgcct gtccaagatt
     2881 gcccatcatc cacaatgtga gttgtcgtag ctgctgctca tcgccgatat agctcaggat
     2941 gccgatcgct gcgccgcaca gggcattgat ggcgataccg gcgagtaata attgcatcat
     3001 gctgccatcg cgatggcgct gcgtgatgag aaaaatgatg gtgcagacca ctaaacttcc
     3061 ggcaatggca aacaccattt gctcatagag caccagcacc actgggaagg agaagggcag
     3121 cacaatagca acgccaacca ttaatgcaga accgctactg acgccaagta atccagggtc
     3181 agccattggg ttgcgaaaaa gcccctgcat aattacccct gcggttgcca acgctgcacc
     3241 taccagcacc gccagcaaaa cccgtggtag acgaatattg agccagatat cgcggtattc
     3301 ctcatcaaaa agggcatcaa aactgatctt taacgctccc tgactggcac caaacagcac
     3361 cagagctagc aataatagtg atagccccat aaagatcctg gcggaagaga gtgaatcctt
     3421 gagcacgcaa aactccgtta cgacattacg gcgcggacat accgcgccgg gggatcacgc
     3481 cggtttcagg tccggttcaa tcagactgta gacctgctgg ttaagtgctg gcgcatttgc
     3541 cagttcatgg atatgtgcgg cgacatcagc acggttaaca aaaccatgac actcctgatt
     3601 ttgtatccgt tgtgctttgc ccgttgccgc accatcaagc agaccgccgg ggcggagaat
     3661 ggcataatca agctggctgg tttgcaacca gctttccgcc agcgtttttt cccgtaccgc
     3721 ctgaccaaac gcagcctttg cccgctctga taaaaacggc cagctatcgc cgcatcccag
     3781 tgacgtcacc agaatcatgc gtgtgatgcc tgctttctcg gcctcgtcaa tcactgttct
     3841 gtgcgccaga taatcctgag ctccgcccat tgttgagatg ataagcgcat ccgggccagc
     3901 tgcgcggcaa gcagccgcga ctacgctggc atcacaggcg tcgcctgtaa aaacctgtac
     3961 gccttgttgc gccagtttcg tggcggcatc ggcatgacga atgacagcaa cgaccggacg
     4021 ttgttccgcc agcgccagtt caagcgttct ggcacccaca ccttttccgc ctgcgccaaa
     4081 gagtagccac ggtgtcatca ggcgtgttcc tttaacgatg ctgccagagc atggaaggcg
     4141 ctgacttgtt cgctcaggag ctgacgatga tcatccctac cgaggaagat tttcagcatt
     4201 gcgctgcctt ccttattgaa aaagagaata gaggcggtgt ccatgcccat aaatttacgt
     4261 tcgatcaagg caatatgcgt gcagttttct gctttgatat gcccggacat gccgtgcttt
     4321 ccgcgtaggt tgaaatagcc gtggcgatgg aaacccgaag gcagttcgcc gctaaattcg
     4381 agtattacat cggtagtatg tactaacgtg gtgacgttgc cccattcgca gacggtatcc
     4441 cagacagtat cgaatttatc acctgacaca acagtcggag agggcagatt tttcactacc
     4501 tccagcagcg tggtgttgta ttgttcggcg atcgcctcca gcgtaccgtc tggctccgtt
     4561 ttcaaaaatt cctgtaacga gacatggctc ataaatcact cctgttatgg tttttcaagc
     4621 gcaattgctg gcgcattaag tacctgaatc agttcattaa gagactgcaa aatgttgctt
     4681 gcccagaaac gaccttcatt ggtcaaccgc agacacgttg acgcatcgcg gcttaagcct
     4741 ttttggtgcc actgggccag taacggcgcg agtttttcgg catgtggcgt aagttcgtcc
     4801 agcggtacac gcgccgtttc aatgcccgac tgcaaggtat gacgccactg cgcgttacgt
     4861 tcggcgttac gcatgatcat catcagcggt tttttgcctg ctgcgacgga ttcatgccag
     4921 gtctgaagat tgcgttcgtt catccaggag tacccattaa tcgaaccgcc ggctccggaa
     4981 ccaaaggcca gacaatcggc accttgttta atcagcaggt tatagagatt gcgttcgcgt
     5041 gtggtacggc cccagtggct gttactgatg cagcgccaac cggcatcgtc cataaaatca
     5101 cacccttgca ggtaaagatc gcgacgttct gccggagagg gcacggtagt acgcccattt
     5161 tccacggctt tgcccagcgg tgtattgggc aggacattga gcgcatagag atcgacgccg
     5221 tcgagaccga tatcgcgggc aatagccaga tcttctcccc aggtttgcgc atcctgaccc
     5281 ggcagaccaa acagcaggtc acagaccacc gcggcacgat cgcgtttgac caggctttcc
     5341 ataaacgcaa tagcggttgg gccatccgag gtgcgggcca ttttcttgcg gattttgctg
     5401 ttaaacgact gaataccaat tgagaagcgg tttgctcctg catcaaggca agcatcgatt
     5461 cgctcagcgt caaaattcag tacccggcct tcgatagtga tttcacaatc aggggccagt
     5521 ggcagctttt ctcgtaatgt ggtgatgatt ctggccaaat cgtgtgctga aagcgcggaa
     5581 ggcgtgccgc caccgaaata gaccgcgtgg atgggggccg actgatgcaa tacgctgtct
     5641 gcttccattt ctatttcacg aatcagggcg tcggtataat gagcacacgc atcttcatta
     5701 aaacggttct gataaaaacc acaaaacgtg cagtgtgtcg cacaaaaagg aatgtgtagg
     5761 taaaccagtc gcttacgtgg tgataccgtt tgattgatca tctcttgcca ggtctgtgcc
     5821 agttgctctt tggcaaccgg aatggcccca cgaaacggca tcatggcgcg cctgtcttta
     5881 aagggctgat caccgtctaa agcaaaatgt ggcgtgagat ccagggtgtt atttgtgttc
     5941 attcgtctga tttaaatcca tttattgcgg ccaaagctgc tggtgtaaag actcaacgac
     6001 atcagcaata cgcggaccca ttcccagaat caggttttga tcaacggtga taatgcgctg
     6061 gtttttccat gcggcagtgt gggtaattcc ggcaatagaa cgtagccgat taatatcgcc
     6121 atcgaccatt tgtgaggtga cgacaatcac ttcagggtta gccgcaatca gcgattccgc
     6181 gctgtagctt ttgtactgct ggtgggtagc gacgttttct gctccggcaa gcgaaagaat
     6241 ggcgtccgcg acgctgcctt taccggcaac ctgtggcgca cttccgcctg cggagagaat
     6301 aaacatggct ttaaccgggg cttttttggc cgccacgttt tgctgtactc gctccaggcg
     6361 ttggttgatc tgtgtcacca gcgcctcgcc ttgttcagga acctgtaacg tttttgccag
     6421 ttgacgaatg ttggcgtaca tctgctcaag ggtggctggt acacgcggca gagtgacgac
     6481 gttgactttt tgcgcccgca gttggtcgag cacaatttgc ggtcctgcat cctgccaggt
     6541 aattacgcta tccgggcgaa gtgacaaaat gccttcgctg ctgagttgtt tccagtaacc
     6601 aatatgcggc agtttggcgg tttctggtgg ataggatgtc gtttcatcga caccaaccac
     6661 gcgtttgcca gcgcccatcg cgtagattag ctccgtcagc gatcctcctg cgaccacgat
     6721 acgttcggca gccgttacgc taaaagtcca gctaagagcc agtaatgccg taaacaattt
     6781 ttttcgtttt accatggtct tcgcggcaga ggccacggct gaaatacata acgttagtgg
     6841 ggaaaaaaga aacggcctgg tgatgatcct tggcataatg tgagaactct tatcaaattg
     6901 agaatgatca tgattatcat ttgagttata tatgaaatac aaccgtcgat tcaatacgca
     6961 cggcgttaca agcgtattta tttttgattt ttatcaagat gcttcgtcag ctaatgttct
     7021 tgttaccgat taaatttcag cgcgggaaaa agctgagaac ccggagatat cgcggcttgc
     7081 agagcaaaga gaagagaaaa ttatcactgt taacgacaac gttttgtgat gaggttgtgg
     7141 ggggatagcc ataaacacag gatggttttc tggatgctgg atagatttca taaccccctg
     7201 tttattgtgt taatggcggt taaaaaaagc ttgatcataa aaaattgatc gataataatt
     7261 ctcattatca cgtttgagtt taacaaaata tgacatcatg gataacaaaa tcatatcttt
     7321 tgtaagggaa aacgcacagc actcttcact tcagacgtgg cgtactactt tgcctgacaa
     7381 agagggcgtg cttttgctga attatcagaa atattcggca attttacggg atatatacgc
     7441 taatagcttc ccgtggtgat atctaatcat ttgaattgta aataaattta tttttcgcca
     7501 gccaccccgg tattaaccca ggcagccagc attttttatt ttcctcatgg agaatcgcta
     7561 tgtcacgtcc gcaatttacc tcgttgcgtt tgagtttgtt ggctttggct gtttctgcca
     7621 ccttgccaac gtttgctttt gctactgaaa ccatgaccgt tacggcaacg gggaatgcac
     7681 gtagttcctt cgaagcgcct atgatggtca gcgttatcga cacttccgct cctgaaaatc
     7741 aaactgctac ttcagccact gatttgctgc gtcatgttcc tggaattact cttgatggta
     7801 ccggacgaac caacggtcag gatgtaaata tgcgtggcta tgatcatcgc ggcgtgctgg
     7861 ttcttgtcga tggtgttcgc cagggaacgg ataccggaca cctgaatggc acttttctcg
     7921 atccggcgct gatcaagcgt gttgagattg ttcgcggacc ttcagcatta ctgtatggca
     7981 gtggcgcgct gggtggagtg atctcctacg atacggtcga tgcaaaagat ttattgcagg
     8041 aaggacaaag cagtggtttt cgtgtctttg gtactggcgg cacgggggac catagcctgg
     8101 gattaggcgc gagcgcgttt gggcgaactg aaaatctgga tggtattgtg gcctggtcca
     8161 gtcgcgatcg gggtgattta cgccagagca atggtgaaac cgcgccgaat gacgagtcca
     8221 ttaataacat gctggcgaaa gggacctggc aaattgattc agcccagtct ctgagcggtt
     8281 tagtgcgtta ctacaacaac gacgcgcgtg aaccaaaaaa tccgcagacc gttgaagctt
     8341 ctgatagcag caacccgatg gtcgatcgtt caacaattca acgcgatgcg cagctttctt
     8401 ataaactcgc cccgcagggt aacgactggt taaatgcaga tgcaaaaatt tactggtcgg
     8461 aagtccgtat taatgcgcaa aacacgggga gttcaggcga gtatcgtgaa cagataacaa
     8521 aaggagcaag gctggagaac cgttccactc tatttgccga cagtttcgct tctcacttac
     8581 tgacatatgg cggtgagtat tatcgtcagg aacaacatcc gggtggcgcg acgacgggct
     8641 tcccgcaagc aaaaatcgat tttagctctg gctggctaca agatgagatc accttacgcg
     8701 atctgccgat taccctgctt ggcggaaccc gctatgacag ttatcgcggt agcagcgacg
     8761 gctacaaaga tgttgatgcc gacaaatggt catctcgtgc ggggatgact atcaacccga
     8821 ccaactggct gatgttattt ggctcatatg ctcaggcatt ccgcgccccg acgatgggcg
     8881 aaatgtataa cgattctaaa cacttctcga ttggtcgctt ctataccaac tattgggtgc
     8941 caaacccgaa cttacgtccg gaaactaacg aaactcagga gtacggtttt gggctgcgtt
     9001 ttgatgacct gatgttgtcc aatgatgctc tggaatttaa agccagctac tttgatacca
     9061 aagcgaaaga ttatatctcc acgaccgtcg atttcgcggc ggcgacaact atgtcgtata
     9121 acgtcccgaa cgccaaaatc tggggctggg atgtgatgac gaaatatacc actgatctgt
     9181 ttagccttga tgtggcctat aaccgtaccc gcggcaaaga caccgatacc ggggaatata
     9241 tctccagcat taacccggat accgttacca gtaccctgaa tattccgatc gctcacagtg
     9301 gcttctctgt tggttgggtc ggtacgtttg ccgatcgctc aacacatatc agcagcagct
     9361 acagcaaaca acctggctat ggtgtgaatg atttctacgt cagttatcaa gggcagcagg
     9421 cgctcaaagg catgaccact actctggtat tgggcaacgc cttcgataaa gagtactggt
     9481 cgccgcaagg catcccacag gatggtcgta acggaaaaat tttcgtgagt tatcaatggt
     9541 aatcatctgc cccgatattt cggggcattt atctggaagg aagagagaca atgaaccact
     9601 acacacgctg gcttgagtta aaagaacaaa atcccggaaa gtacgcgcgt gacatcgcag
     9661 gtttaatgaa tatcagcgaa gcagaactgg catttgcacg cgtcacgcac gacgcgtggc
     9721 ggatgcgcgg cgatattcgt gaaattctgg cggcgctcga aagtgttggc gagaccaaat
     9781 gtatttgccg taatgaatac gcagtccatg aacaagttgg tgcgttcaca aaccagcatt
     9841 tgaacggaca tgccggattg atcctcaatc cgcgcgcgct ggatttacgt ctgtttctca
     9901 atcaatgggc cagtgttttc cacatcaaag aaaacacggc tcgtggcgaa cgccagagta
     9961 ttcagttctt tgatcatcag ggcgatgcat tactaaaagt ttatgccacc gacaataccg
    10021 atatggcggc atggagtgag cttctggcac ggtttatcac cgatgagaat acgccgcttg
    10081 agttaaaagc cgttgatgcg ccagttgttc aaacgcgagc cgatgccagt gtggtcgagc
    10141 aagagtggcg ggcgatgacc gacgttcatc agttttttac gttgctcaag cgccacaacc
    10201 tgacgcgcca acaggcgttc aatctggtgg cagacgattt ggcctgcaaa gtatccaaca
    10261 gtgcgttggc gcaaattctt gaatctgcac agcaggatgg caatgaaatc atggtgtttg
    10321 ttggcaaccg tggctgcgta cagattttca ccggtgtggt agaaaaagtg gtgccaatga
    10381 aaggttggct gaatattttc aacccgacgt ttacccttca tctattagaa gagagcattg
    10441 ctgaagcctg ggttacccgt aaaccgacca gcgatggcca cgtaaccagc ctggaattgt
    10501 ttgcccatga tggtacgcag atagcgcaac tttatggtca acgtacagaa ggtgaacagg
    10561 agcaagcgca atggcgtaag caaattgctt cgttgatacc ggaaggcgtt actgcataac
    10621 gtataattaa cgggaggtct ggctgttgct gggcctcttt atattgttca atcgttcaca
    10681 ccagataatt aatatgctga tggcgtacca gctcattgat ccgcttaaga ttgaggatca
    10741 tcatgatatt gtgtttatgg cagtagaacg tttttagcga tattttcagt ttctccagaa
    10801 tatcttccgt tgtcatttga ttcgcaatac agctcagaac ctgatgctgc ttgcgcgaga
    10861 gggacacatc cgcttcaggc acagagcagt aactgccatt gatgagtgcg acaaagtcga
    10921 tgatggcgat agttcgcgga acaaaggtca ccggtacttt ccccaggcag cgtttaatat
    10981 taaaggggga gaaaataatt acatggacag gttttaatcg ttcaatttga gtgagaaaat
    11041 tagaatgaaa tacattattc attaatgtat caataatgac gaaggcattc tgatgcaaca
    11101 ttacgtcgat ctcttcttcg ttctgtatat gaacgacatt acctttactc agaatgtttt
    11161 tcatcgcggt gaaaaacatc gtatccctgg taattataag aaacataacc gactcctggt
    11221 acggacatta actttataat cagtttgcca aaggtaatta acaggacagg gcttataata
    11281 atagttagct tactgtgtat taatttgatg taatgcatga tgcagatgct gagaaagcat
    11341 ctgcatcttt cggtggtgtt atttgaccag ctcaggtgtt acctgactca ccgcattggt
    11401 gtagtaagga gccccccagc ccggttccgg ccagaatgca ccatagccgt aatcccacag
    11461 gttataggtg gtattaacca cttctctcaa atgccacacc tggatgccct gcatattcac
    11521 ttccaggaag ttatacggga ctttattgat aaagccaggt tgttcaccct gaatggtacc
    11581 gaggatggta acaaagtgat tacgatagtt cactggatca aggaagccgc tttgtctggc
    11641 gagcagtcgg ccctgatagt tggcttcaat atcaggcttc gcatagctat ccaacggtaa
    11701 tacagcgatt tctaacaacg tatccgtttt gccattgata acgttgataa ccttcccacc
    11761 aaagcgcgct tgttgaccaa catataaccc cggctggtta tgaacagcaa caaaactttt
    11821 ttgaatatca ggttggttat tgcctttgat attttgcgga attgaactac atgcggcaag
    11881 caaaaatgaa aggctgagga tgagtgcacc ttttgtcatg ttcatgttac tatccttatc
    11941 aacaaatatt aaaaccataa tagatatttc tttatagttt tcatctgatt ctgaggcgat
    12001 atgcctgata gtaacgtaaa cctgtctgat gtttatatct cttctgttta gttgagctca
    12061 gttaattttt ggacgtggca tgagtatcca ttgttccata atctatattc agaggttttt
    12121 aatcttttcg tgtctaatgt tatttatctg attaagtatt gattgtgtct tttgcggaag
    12181 tggtgtcgag cttatcgttt caatgttatt aataagactt acaagttgag cctcaacttt
    12241 gagtagcctg catttgtata tgtggtgttc gtatggcacg cattaatttg ttggcgttgt
    12301 tgcgctaaat caaattgttc agaggtgtat ccattctcca gagaatgggg aattaatggg
    12361 gtgttgttct tcccggttat cacaaggcca cttcggtgtg tctggcgttt taacattatc
    12421 ggtatttgtt gtgcagttat aaatatcacc gttatatgta tttattacac ctggggtaaa
    12481 gtcaattgac ataacattcc ttattcatca tttgttttat gctaagattt ttaaattgga
    12541 taattagaac aggaatggat tttcttctac atacagtaaa tataatgcat catatcttcc
    12601 tgatataatt acctttgact tcccttttat ttccatctgg taatatttga gtgcaaaaaa
    12661 ggcaataata gtatcgcaaa agaaaacgtt aacaactatc gaaaatgctc gttttaaaaa
    12721 gttatgatgt tttctgatag tgtgtgaagt gatatttttg tgatgtttta gatatgtaat
    12781 aataaaatgg gttaacgtat gttgaaatta tttattgtgt aaaccactga tagatcataa
    12841 tccagtacat ctttacagtg ctgcactgta ccgctgataa ctaagcagct cactgataca
    12901 ccatgctgga cgcgataatt ggaatctcac gcagggagta ctgtgtaaat agcaaaatcg
    12961 cctgatgcgc tacgactgtc aggcctacaa ataacataca attcatcgaa ttatcataac
    13021 gttgtaggtc ggataaaacg cgccagcgtc gcatccgaca taaataaagc gcatttttct
    13081 aacaacctgt gggggaatat agccgcttta tttcaggcga ttacccgcct catcaaccac
    13141 tttctcgcca tcttccttgg tgaacgcgcc tttttgcgca tctggcagaa tttccagcac
    13201 gacttctgaa gggcggcaca ggcgagttcc ctgtggcgtc accacaatcg ggcgattaat
    13261 cagaatcggg tgctgaagca taaagtcgat taactgatcg tgagtaaatt tatcttctgc
    13321 aaggcccagt ttctcatacg gctcgacgtt tttacgcagc aacgcgcgta cggtaatccc
    13381 catatcggca atgagtttta ccagctcatc gcgcgtcggt ggtgtttcca gataatagat
    13441 aatggtcggt tctgtaccgc tgttgcggat catctccagc gtattacgcg acgtaccgca
    13501 ggccgggttg tgataaatgg taatgttgct catatcagta tctcattacg atgtgaaaga
    13561 gaggtgtagc gccagcgcca ccagcgttac aaaccaaagc ggacaagtca aaaacacata
    13621 tggtttaacg aatatgtaag tgtagagaaa ggtgtaggtg tgaattaagc gttgggcatt
    13681 catttgaaag gaggtctgaa tcaggacgta aaaaaaccga cttcgcgtcg gtttttttac
    13741 tgttcagccc tgagttggtg gctctggctg gagtgagata gccatcatct aacgcatcaa
    13801 tgctaatacc atactgaaac tattgcaagg acatgctggt tttataaccc gcaagtactg
    13861 tatgattatc cagttagctc tgagacattt tcactctggc aatgcgcata aacgctttca
    13921 aagtcctggt ctgaagtacg ggtggtgccg ttaactgatg ctctggccgg agtgagatag
    13981 tcgtcatcta acaatgagac atgcgccgtg acaggcaatg gatgagtaag cggatgcatt
    14041 ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt cttggggcgt
    14101 taattgcgct gctggaactg atccgctttc tgcttcagct tctgaactga tagcggaaac
    14161 gtagctaagg gctaagagca cactactctt agccctttaa catttaacgc attgtcacga
    14221 actcttctgc cgctgtcggg tgaatggcaa ccgtgttgtc gaagtctttt ttggttgcac
    14281 ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaaa ccaatgccgt
    14341 gaataccgac aatcttctct tccggtccaa cgcataccag tttcatgcga cacggctggc
    14401 ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact ttcacctgat
    14461 cgtcgccgta ctgctcgcgc gcctgcggct ccgttaaacc aacagtacca atcggcggat
    14521 ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc ttgttattaa
    14581 acaggcgttc agagagacgg cgacctgctg caacagccac cggtgtcagt tccactgcac
    14641 ccgtgttatc gcccaccgcg taaatacctt caacgttggt attttgatat ttatcgacga
    14701 cgatatagcc tttttcgttg gttttaacgc cagcggcttc caggttgatg ttgtcattgg
    14761 caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga ccatcttcca
    14821 gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg gcgttggtgt
    14881 gcaactgcgg gccttcggcg ttcatcactt caaccagcgt ttcagagatc atcgggtcga
    14941 agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt cttcgcaccg aggccgttaa
    15001 tcacgcccgc cagttcaacg gcgatgtaac ccgcgccaac aaccgctaca cgctctggca
    15061 aagccggaag ggcgaagaag ccatcagaat caataccgta ttccacgccc ggaatatccg
    15121 ggtggctcgg acgaccaccg gtggcgatca gaatatgatc ggccgtgatg gtttcgccgt
    15181 ttacctccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca tcaacgttat
    15241 ttttaccgag cacgttctca taggaagtat gaatacggtc gatataggcg gtacggctgg
    15301 cgatcaacgt ttcccagttg aatttattga tagtggtatc aaaaccataa tccgggccgt
    15361 acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc ggcacacagc
    15421 caacatttac gcaggtgccg cccagctctt tggcttcaat cagtgcgcat ttctggccgt
    15481 acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg gcgatgtaat
    15541 catagtgttt agtcatgaca tattgtcctt atcgttgatt accgcgattg tagcgcgaga
    15601 cgtaataggt gccagcaatg gctgcaatta ctctggcacg atccagctta cggtggcgtg
    15661 cccggtgcct gccggaacca gtttgctgtg cagccacggc agcacgttat tcatctgctg
    15721 ttccagcttc cacggcgggt taatcacaat catgccagaa gcggtcatgc cacggcgatc
    15781 gctgtctggc agcaccgcca gttcaatttg cagaattttg cgaatgtcgg tcgcttccag
    15841 gtcgtggatc atgcgcttaa tttgctggcg cagcaccact ggataccaca atgcgtaagt
    15901 gccagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct gatagtcggt
    15961 tttcatttca tacggcgggt cgataaggat taaaccacgg cgggaaaccg gcggcagctt
    16021 ggccttaagc tgctggaaac cgtcggcttt ttcgacgcgc gcacggctgt ctttctgaaa
    16081 ttcagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca ggctgtcctg
    16141 ttcacgcagt agctggcgag caatcaacgg cgaaccgggg taataacgca actgcccgct
    16201 acggttgaag tgttttacca cattgatgta cgcctccagc tccgcaggca aatcgtcctg
    16261 ctgccagata cgggcgatac cttcgagata ttcgccggta cgctcggcat gttcgctgcc
    16321 taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt tatctttctc
    16381 tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg cgtggttgcc
    16441 agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa gtaaaaatcg
    16501 gcccgtattc ggggcgcaca aaagcctatc aggacagtat accgaaaaga ggccgccgcc
    16561 gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct acaaacatta
    16621 tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc taatgacgaa
    16681 tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg aacatgtcgt
    16741 tccagccgtg actaaggcgc tgaacgactg ccgcgaaaat gtggagcgcg tagtagcgca
    16801 aggggcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg acgatgtgct
    16861 ggggcgtatc ttctccccgg tcagccatct gaactcggtg aaaaatagcc cggaactgcg
    16921 tgaagcgtac gaacaaaccc tgccgctgct gtcagaatac agcacctggg tagggcaaca
    16981 tgaagggctg tataaggcat atcgcgacct gcgtgatggc gatcattacg ccacgctgaa
    17041 cacggcgcag aaaaaagcgg ttgataacgc actgcgcgac ttcgaactct ctggcatcgg
    17101 tctgccgaaa gagaaacagc agcgttacgg cgaaattgct acccgtcttt ctgaactggg
    17161 caaccagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac tcgttaccga
    17221 cgaagcggag ctggcgggga tgccagaaag cgcactggct gcggcaaaag cccaggctga
    17281 agcgaaagag ctggaaggtt atttgctgac gctggatatc ccaagctacc tgccggtaat
    17341 gacctactgc gacaaccagg ctctgcgcga agagatgtat cgcgcttata gcactcgtgc
    17401 ttccgatcaa ggtccgaacg ccggtaagtg ggataacagc aaggtgatgg aagagatcct
    17461 cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct tcaaatcact
    17521 tgccactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg atctggcaaa
    17581 acgcgcgcgc ccgcaaggcg aaaaagagct ggcgcaactg cgcgccttcg ccaaagccga
    17641 atttggcgtc gatgagttac aaccgtggga tatcgcttac tacagcgaaa aacagaaaca
    17701 gcacctctac agcatcagcg atgaacaact gcgtccgtac ttcccggaaa acaaagcggt
    17761 taacggcctg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag agcgtaaaga
    17821 tgttgatgtc tggcatccgg atgtacgttt cttcgagctg tatgacgaga acaacgaact
    17881 gcgcggcagc ttctacctcg acctgtatgc ccgtgaaaac aaacgcggcg gggcgtggat
    17941 ggatgactgc gtaggccaga tgcgtaaagc cgacggttcg ctgcaaaaac cggtcgcgta
    18001 tctgacctgc aacttcaacc gcccggtaaa tggtaaaccg gcgctgttta cccatgacga
    18061 agtgctcacc ctgttccacg agttcggtca cggcctgcac catatgctga cccgcattga
    18121 aaccgctggt gtttccggga tcagtggggt gccgtgggat gcggtcgaac tgccgagcca
    18181 gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctccg gtcactatga
    18241 aaccggcgaa ccactgccga aagagttgct ggataaaatg ctggcggcga agaactacca
    18301 ggcggcgctg tttatcctgc gccagctgga gttcggcctg ttcgatttcc gcctccatgc
    18361 cgagttccgt ccggatcagg gggcgaaaat cctcgaaact ctggcagaaa tcaagaaact
    18421 ggttgccgtg gtaccgtctc cgtcatgggg ccgtttcccg catgctttca gccacatttt
    18481 tgccggtggt tatgcggcag gttactacag ctacctgtgg gccgatgtgc tggcggcaga
    18541 tgccttctcg cgttttgagg aagagggcat tttcaaccgt gaaactgggc agtcgttcct
    18601 cgacaacatt ctgagccgtg gcggttcaga agagccgatg gagctgttca aacgcttccg
    18661 tggtcgtgaa ccacagctgg atgcgatgct ggagcattac ggcattaagg gctgatcatt
    18721 cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta tctgttctgg
    18781 cggcccgctg ggggctggaa cacgatgaag acaacctgat ggcgctggtg ttaacgccgg
    18841 agcatctgga actgcgcaag cgtgatgagc cgaaacttgg cggcatcttt gttgattttg
    18901 ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggagag gcggtggcga
    18961 aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc gcaggattag
    19021 ggcgcgatgc ctttgtgctg gcttcggtcg gctgccgcgt gcggatgctg gagcgtaatc
    19081 cggtggttgc cgcgttgctc gacgatggtc tggcgcgtgg ttatgcggat gcggaaatcg
    19141 gcggctggtt acaggaacgt ttgcagttga ttcacgcctc cagtctgacg gcgctgagcg
    19201 atatcacccc gcgtccgcag gtagtttatc ttgacccaat gttcccgcat aagcagaaaa
    19261 gcgcgctggt gaaaaaagag atgcgcgtgt ttcagtcgct ggtggggccg gatcttgatg
    19321 ccgatggatt gttggagcct gcgcggttgc tggcaaccaa acgcgtggtg gtgaagcgtc
    19381 cggactatgc gccgccgctg gcgaatgtcg ccacgccaaa cgcggtagtc actaaagggc
    19441 atcgctttga tatttatgcc ggaacgccgg tgtaaaagta aagcggggta gcgataacgc
    19501 taccccgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat gcgttttagc
    19561 cacggcacca tcaatagcat cactacggca acgcccagcg taaccaggcc aatcttacca
    19621 aacacgttgg tatagacggg caacgtctca agtggatcgg taatgttgtc tggtactgcg
    19681 gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag gaaccacatc
    19741 ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc caggccaagg
    19801 gcgctaataa acagttcacc taagctctgg aataagtaca ccagcacgat aaaccatggc
    19861 gatgtcagcc cctgggcatc cgcaaaccac attccagccg ctgcagccgt caaaaagccc
    19921 agtgagcaca taaacatgcc gagagtaaac ttcatcggca tcgagagatc tttgcctttg
    19981 ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac ccagaacggg
    20041 ttaagcgcct ggaagctgac cgggttgatg gaaaagccga gaatttcatg atgcacgttg
    20101 ttgatggcaa agaagttcag cgacgttggc atctgggcgt agagaatgta aaacaccacc
    20161 gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt atccagcttg
    20221 aatgcctgac gaaagaagat gatggtgacg acgatggaga gaacaatcag caccagattg
    20281 gcgacttcta cgttgtgcat cagccaggcg cagacgaaga tcatcaccac gctgccaagt
    20341 aatacgtaca acagtttact gaagctcatc ggccggaagt cgggttcaga accaatatct
    20401 ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc tgcaccgcac
    20461 aggttgtagg tgactgaata gccgaattta tcagcgatca caggggccag cgataacgct
    20521 atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc aagccgcgga
    20581 tctttcggcg ggtagcactt cgaaagcaag ctggctgggt tagctttaaa caggccgtta
    20641 ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag cgacatgccg
    20701 gtcatgaagt agccaatcgc cagcacaagt gctccgagaa caatggtgcg tttggttccc
    20761 agcaggtggt cgccaacata gccaccaatg gaaatgaggc catagaccag cgcagcaaaa
    20821 gcaccaaaag tgacaaaagc ctgctcttgc gagaacccaa gctgtttaac gaagaaaacc
    20881 gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac aaaaaagatc
    20941 atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt catatttatt
    21001 aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc gaaaaagttt
    21061 acatctgtca gagattctta atattgatg
//
LOCUS       DAZFJL010000083        20734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFJL010000083 DAZFJL010000000
VERSION     DAZFJL010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.083"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..1227)
                     /locus_tag="ACHJ8C_002978"
     CDS             complement(<1..1227)
                     /locus_tag="ACHJ8C_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HFO5552800.1"
                     /translation="MFTNVNVDCCKTPGCKNLGLLNSQDYVAQGKNILCRECGYLFPV
                     ISEQSLNIYRNIVNHSWRGLICQCSTCGGTSLKKYGYSAQGQRRMYCHHCEKTFITLE
                     HVITTPRGALLALMIEQGEALADIRKSLLLNSTGLSRELLKLAREANYKESRQCFPAS
                     DITLSTRAFRVKYNGSNNSLYALVTAEEQSGRVVAISTNYSPSAVEQHYQYTSNYEER
                     MSPGTLAHHVQRKELLTMRRDTLFDIDYGPAVLHQNDPGMLVKPVLPAYRHFELVRIL
                     TDEHSNNVQHYLDHECFILGGCLMANLQHIHQGRCHISFVKERGVAPATIDFPPRLFL
                     SGGVRNNVWRAFSNRNYSMAVCNLTGSKKVREMRHATLNSATRFIHFVENHPFLISLN
                     RMSPANVVSTLDILKHL"
     gene            complement(1356..2849)
                     /locus_tag="ACHJ8C_002979"
     CDS             complement(1356..2849)
                     /locus_tag="ACHJ8C_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5552801.1"
                     /translation="MQKTLMASLIGLAVCTGNAFSPALAAEAKQPNLVIIMADDLGYG
                     DLATYGHQIVKTPNIDRLAQEGVKFTDYYAPAPLSSPSRAGLLTGRMPFRTGIRSWIP
                     SGKDVALGRNELTIANLLKAQGYDTAMMGKLHLNAGGDRTDQPQAQDMGFDYSLVNTA
                     GFVTDATLDNAKERPRYGMVYPTGWLRNGQPTPRADKMSGEYVSSEVVNWLDNKKDSK
                     PFFLYVAFTEVHSPLASPKKYLDMYSQYMSAYQKQHPDLFYGDWADKPWRGVGEYYAN
                     ISYLDAQVGKVLDKIKAVGEEDNTIVIFTSDNGPVTREARKVYELNLAGETDGLRGRK
                     DNLWEGGIRVPAIIKYGKHLPQGMVSDTPVYGLDWMPTLAKMMNFKLPTDRTFDGESL
                     VPVLEQKALKREKPLIFGIDMPFQDDPTDEWAIRDGDWKMIIDRNNKPKYLYNLKSDR
                     YETLNLIGKKTDIEKQIYGKFLKYKTDIDNDSLMKARGDKPEAVTWG"
     gene            complement(2870..3631)
                     /locus_tag="ACHJ8C_002980"
     CDS             complement(2870..3631)
                     /locus_tag="ACHJ8C_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO5552802.1"
                     /translation="MSAKRRLLIACTLITAIYHFPAYSSLEYKGSFGSINAGYADWNS
                     GFVNTHRGEVWKVTADFGVNFKEAEFYSFYESNVLNHAVAGRNHTVSAMTHVRLFDSD
                     MTFFGKIYGQWDNSWGDDLDMFYGFGYLGWNGEWGFFKPYIGLHNQSGDYVSAKYGQT
                     NGWNGYVVGWTAVLPFTLFDEKFVLSNWNEIELDRNDAYTEQQFGRNGLNGGLTIAWK
                     FYPRWKASVTWRYFDNKLGYDGFGDQMIYMLGYDF"
     gene            4246..4398
                     /gene="mokC"
                     /locus_tag="ACHJ8C_002981"
     CDS             4246..4398
                     /gene="mokC"
                     /locus_tag="ACHJ8C_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO5552803.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRIGQTEVAVF
                     TAYESE"
     gene            complement(4503..5633)
                     /gene="dnaJ"
                     /locus_tag="ACHJ8C_002982"
     CDS             complement(4503..5633)
                     /gene="dnaJ"
                     /locus_tag="ACHJ8C_002982"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO5552804.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(5722..7638)
                     /gene="dnaK"
                     /locus_tag="ACHJ8C_002983"
     CDS             complement(5722..7638)
                     /gene="dnaK"
                     /locus_tag="ACHJ8C_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO5552805.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            8010..8414
                     /gene="yaaI"
                     /locus_tag="ACHJ8C_002984"
     CDS             8010..8414
                     /gene="yaaI"
                     /locus_tag="ACHJ8C_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO5552806.1"
                     /translation="MKSVITISASLAISLMLCCTAQPNDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASIYFKATRSASQTLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMTTLKIIGDD"
     gene            8440..9153
                     /gene="msyB"
                     /locus_tag="ACHJ8C_002985"
     CDS             8440..9153
                     /gene="msyB"
                     /locus_tag="ACHJ8C_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO5552807.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPERHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            9302..9868
                     /gene="satP"
                     /locus_tag="ACHJ8C_002986"
     CDS             9302..9868
                     /gene="satP"
                     /locus_tag="ACHJ8C_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO5552808.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGIFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            9927..10025
                     /locus_tag="ACHJ8C_002987"
                     /pseudo
     CDS             9927..10025
                     /locus_tag="ACHJ8C_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10073..10660)
                     /gene="mog"
                     /locus_tag="ACHJ8C_002988"
     CDS             complement(10073..10660)
                     /gene="mog"
                     /locus_tag="ACHJ8C_002988"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO5552809.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARREVSE"
     gene            complement(10775..11728)
                     /gene="tal"
                     /locus_tag="ACHJ8C_002989"
     CDS             complement(10775..11728)
                     /gene="tal"
                     /locus_tag="ACHJ8C_002989"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO5552810.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            12007..13437
                     /gene="yaaJ"
                     /locus_tag="ACHJ8C_002990"
     CDS             12007..13437
                     /gene="yaaJ"
                     /locus_tag="ACHJ8C_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO5552811.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYAIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSFPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPEIGRQLSPDAWDDVSQE"
     gene            13507..14283
                     /gene="yaaA"
                     /locus_tag="ACHJ8C_002991"
     CDS             13507..14283
                     /gene="yaaA"
                     /locus_tag="ACHJ8C_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO5552812.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISSLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(14333..14629)
                     /locus_tag="ACHJ8C_002992"
     CDS             complement(14333..14629)
                     /locus_tag="ACHJ8C_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO5552813.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPSPRHNKHNDHRGDHRPGPDKHHR"
     gene            complement(14843..16129)
                     /gene="thrC"
                     /locus_tag="ACHJ8C_002993"
     CDS             complement(14843..16129)
                     /gene="thrC"
                     /locus_tag="ACHJ8C_002993"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO5552814.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEYSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(16130..17062)
                     /gene="thrB"
                     /locus_tag="ACHJ8C_002994"
     CDS             complement(16130..17062)
                     /gene="thrB"
                     /locus_tag="ACHJ8C_002994"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO5552815.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(17064..19526)
                     /gene="thrA"
                     /locus_tag="ACHJ8C_002995"
     CDS             complement(17064..19526)
                     /gene="thrA"
                     /locus_tag="ACHJ8C_002995"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO5552816.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(19552..19671)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(19607..19672)
                     /gene="thrL"
                     /locus_tag="ACHJ8C_002996"
     CDS             complement(19607..19672)
                     /gene="thrL"
                     /locus_tag="ACHJ8C_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO5552817.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(19886..20572)
                     /gene="yjtD"
                     /locus_tag="ACHJ8C_002997"
     CDS             complement(19886..20572)
                     /gene="yjtD"
                     /locus_tag="ACHJ8C_002997"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO5552818.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAA
                     RWVAHGSGDIIDNIKVFKTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLHALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         5046 a         5367 c         4914 g         5407 t
ORIGIN      
        1 cagatgtttg aggatatcta atgtagaaac gacattcgca ggagacattc ggttcaatga
       61 tataaggaaa ggatggttct ccacaaagtg gataaaacgc gtcgcactgt tcaatgttgc
      121 atgccgcatc tcgcggactt tcttactgcc agtgagattg catacagcca ttgaataatt
      181 gcggttagaa aatgcacgcc agacattatt tcgtacccca ccactaagga ataatcgcgg
      241 tggaaaatca atggtggcgg gtgccacacc gcgctctttg acaaaggaaa tatggcagcg
      301 accttgatga atatgctgca aattagccat caggcagccg cccaatataa agcattcgtg
      361 atcaaggtaa tgctgaacgt tgttggaatg ctcatcggtc agtattctga ccagttcaaa
      421 atgacgatat gccggaagaa ccggttttac caacattccc ggatcgtttt gatgtaaaac
      481 tgccgggccg taatcaatat caaacaaggt atcccgccgc atagtaagta actctttgcg
      541 ctggacatga tgtgccagcg tccctggaga catacgctct tcatagttcg atgtgtattg
      601 ataatgttgc tctacggcag atggggagta attggttgag atggcaacca ccctgccgct
      661 ttgttcttct gcggtaacaa gagcataaag agagttattg ctaccattat acttgacgcg
      721 aaaagcgcgg gtactcaggg taatatcaga agcagggaaa cactgtcgac tttctttata
      781 gtttgcttca cgcgctaatt ttaacagttc acggctaagt ccagtgctgt taagaagtaa
      841 tgactttctg atatccgcaa gtgcctcccc ttgctcaatc atcaatgcta acagggctcc
      901 tcgtggtgtg gtaattacat gttccagagt gataaatgtt ttctcacaat gatggcaata
      961 cattcttctc tggccttgtg cagaatatcc atattttttg agggacgtgc ctccgcaagt
     1021 tgaacattgg caaatcaaac ctctccagga gtgattcaca atattacgat aaatattaag
     1081 cgactgttca gatatcactg gaaacaagta accacattca cggcataaaa tatttttacc
     1141 ctgtgcgaca taatcctggc tattcagcaa ccccaggttt ttacatcctg gtgttttgca
     1201 acaatcaaca tttacgttgg taaacatatc cactccctgc gaatagccgt atcttgatat
     1261 tgttcaacgc gaaaagaacc tgattcaatg cgtagcactg aatcaggcgg gcatagccct
     1321 aaatggcata cattacattt attatgatta tcaggttagc cccaggtcac cgcttctggt
     1381 ttatcacctc tggctttcat tagagaatca ttatcaatat cagttttata ttttaaaaac
     1441 ttaccatata tctgtttttc aatatctgtt tttttaccga tcagattaag tgtttcataa
     1501 cgatcagatt tcagattgta gagatatttc ggtttattat tgcgatcgat aatcatcttc
     1561 cagtcaccat cacggatcgc ccattcatcg gttggatcat cctggaatgg catatcaatc
     1621 ccgaaaatta atggcttttc gcgtttcaat gctttttgct caagaacagg aaccagcgat
     1681 tcaccatcga aagtacggtc tgtaggtaat ttgaagttca tcattttcgc taaagtaggc
     1741 atccagtcca gaccataaac gggtgtatct gaaaccattc cctgtggtag atgtttacca
     1801 tatttaataa tggctggaac acgaattccg ccttcccaaa ggttatcctt gcgaccgcgt
     1861 aatccatccg tttcccctgc caaattcagc tcatacactt tgcgcgcttc acgcgttacc
     1921 ggaccgttat cactggtaaa aataacgatt gtgttatctt cttcacccac agctttgatt
     1981 ttatccagca cttttccaac ctgtgcatcc agatagctga tattggcata atattccccc
     2041 acaccacgcc agggtttgtc tgcccagtcg ccataaaata aatcaggatg ctgcttctga
     2101 tacgcgctca tatattgtga gtacatatcg aggtattttt tgggcgaagc cagggggcta
     2161 tgcacttcgg taaaagcaac atagaggaag aaaggcttgc tgtccttttt gttatccagc
     2221 cagttgacga cttccgaact gacatactca ccgctcattt tatcagctcg tggagtgggt
     2281 tgcccgttac gtagccagcc tgtcgggtaa accatgccat aacgcgggcg ttctttagcg
     2341 ttatccagcg tggcgtcggt aacaaagccc gccgtattaa ccagtgagta atcaaagccc
     2401 atatcttgtg cttgtggctg atcggtgcga tcgccgcctg cattcagatg cagcttaccc
     2461 atcattgccg tgtcgtaccc ttgcgctttg agtagattag caatcgtgag ttcgttacgc
     2521 cctaaggcaa catctttgcc tgaaggaatc catgagcgaa ttccagtacg aaatggcatc
     2581 cggccggtta atagccctgc gcgtgaaggt gaacttaaag gagcaggggc atagtagtca
     2641 gtaaatttga ccccttcctg ggcaagcctg tcgatattag gtgttttaac gatctgatga
     2701 ccatatgttg ctaaatcgcc ataacctaaa tcatccgcca taataatgac taaattaggt
     2761 tgtttagcct ctgcggctaa ggcaggacta aaagcattcc ctgtgcaaac tgcaaggccg
     2821 atcaaactgg ccattaacgt tttctgcata attattccct gataaaaaat tagaaatcat
     2881 aaccaagcat ataaatcatt tgatcgccaa agccatcgta gcccagctta ttatcgaaat
     2941 aacgccacgt cacactggct ttccagcgag gatagaactt ccaggcaata gttaaaccgc
     3001 catttaaccc gttccggcca aattgctgct ccgtgtaagc atcgttcctg tccagttcta
     3061 tttcattcca gttagataaa acaaattttt cgtcaaataa cgtaaatggt aatactgctg
     3121 tccagccaac aacataacca ttccaaccat tcgtttgacc atatttagct gatacgtagt
     3181 caccagattg attatgcaat ccaatatacg gtttaaaaaa gccccactcg ccgttccagc
     3241 cgaggtaacc gaatccataa aacatgtcca gatcgtcacc ccatgagtta tcccattgac
     3301 catagatctt gccaaagaaa gtcatatcgg agtcaaagag tctgacatgc gtcatagcag
     3361 aaacggtatg attacgcccc gctacggcat ggttaagcac attgctttca taaaatgagt
     3421 aaaactcggc ttctttaaaa ttcactccaa agtccgcagt cactttccat acttcaccac
     3481 ggtgagtgtt aacgaagcca ctattccagt cagcataacc tgcatttatt gagccaaagg
     3541 accctttata ttctaatgaa gaatatgcag gaaaatgata gatagctgtt atcagggtac
     3601 acgcaataag aagtcgtctt ttggcagaca tataatatgt tctccattca ttgtttttat
     3661 gattccatag gaattaatac accagacaat tcatttttat taagaatgac caggccagta
     3721 tatttaaaga tgactatcga agcgtgatga agatcgcaga ttacttttgc gaacgggcgg
     3781 agggatatcg tgtttcagaa aatgtgtctg ttttaaacat caccctttga tttataggca
     3841 tttttcatga tgaacttagc attggctgct gaacgtattt ctttatctct ctatagtatt
     3901 acagtaacct ttaataatta atttccttta gccattcact tttgatagag gaaaacacct
     3961 ttttttgtga atccctggat atatttaagt taaataaatt gatgtattga aatattattc
     4021 cgcaaaaatg cgaggcgtct tcagaaaaag aattgataaa acagaagtga attaaaagcg
     4081 cagtataata gcagcgggtg cttgaggctg tctgtctcag gcattggctg aacggcagat
     4141 agagaaaagc cccgagtgat attttaccat caacccgagg ccccctatat gcagaacaca
     4201 tgtagagtgc ctcttactga ccgaaaggtc aaggagaaga gagcaatgaa gcagcataag
     4261 gcgatgattg tcgccctgat cgtcatctgt atcaccgccg tagtggcggc gctggtaacg
     4321 agaaaagacc tctgtgaggt tcacatccga attggccaga cggaggttgc tgttttcacg
     4381 gcttacgaat ccgagtaaga gcaacggcgg ggagtgatcc ccgccacttt acaggtgctc
     4441 gcatatcttc aacgcaccct ggtttttacc ctggcctgcc cacgggcagg cttttgggga
     4501 ggttagcgag tcaggtcgtc aaaaaacttc ttcacgccat caaagaagct ctttgaacgc
     4561 gggctgttgt gctcgccggt tgggccacca aagctttctt gcagctcttg cagcagctgt
     4621 ttctgcttct cgttcaaacc taccggtgtt tcgacaacaa cgcggcatag caaatcaccc
     4681 tgtgcgccac cgcggacaga cttgacgcct ttaccgcgca tacggaacag cttaccggtc
     4741 tgggtttcgc caggcacttt cagtttgacg cgaccatcaa gggtcggtac ttcgatttca
     4801 ccacccagcg ccgccatagc gaagttgatc gggacttcgc aatacaggtt gttgccttca
     4861 cgctcgaaaa tcgggtgctg tttaacctga acctgaacgt acagatcgcc tgccggtgcg
     4921 ccgtgttcac ccgcttcacc ttcgcccgca agacggatgc ggtctccagt gtccacccct
     4981 gccgggattt taacggacag cgttttgctg cgctcaacac gaccatgacc atgacatttg
     5041 ttgcacggat ctttgatcag cgtaccgcgg ccctgacagt gaggacaggt ctgctgcacg
     5101 gcaaagaaac cctggcgcat ctgcacctga ccagaaccat gacaggtcgg acaggtctgc
     5161 ggctgtgtac ctggttttgc accgctaccg tggcaaacgt cacactcttc cagagtcgga
     5221 atgcggatct ctttggtcac gccacgtaca gcttcttcga gggtgagctc catgttatag
     5281 cgtaaatcag caccgcgcgc cgcacgttga cgaccacgtc cgccgccaaa aatatcgccg
     5341 aaaacgtcac caaaaatatc gctgaagtct gcgccgccgc caaaaccgcc gccgcccatg
     5401 ccgccttgct caaacgcagc atgaccatac tgatcgtatg ccgcacgttt ttgcgagtcg
     5461 gtcaaaactt cataagcctc cttgatctct ttaaatttcg cctcggcctc tttgtcaccc
     5521 tggttacggt ccgggtggta tttcatggcc aggcgtttgt aggccttttt gatttcacgc
     5581 tcttccgctg ttttggaaac gcctaaaatc tcgtaataat cttgcttagc catctttttt
     5641 gatttgcccc tagatgaatg cacgggcgga gaggaaattc cccttcgccc gtgtcagtat
     5701 aattacccgt ttatagggcg attatttttt gtctttgact tcttcaaatt cagcgtcgac
     5761 aacatcgtca tctttcgcgt tgtttgcaga agcatcagca ccggcagtct gctgctgggc
     5821 atgttgctgc tgggcgattt ccatcagttt ctgggaaacc tgtgccagtt cctgcatttt
     5881 cgcttcgata gcggctttgt cttcaccttt cagagcagtt tccagtgcag tcagcgcaga
     5941 ctcgatagca gttttgtcgt cagccggcag tttgtcgcct gcttcttcaa cctgcttacg
     6001 ggtgctgtgc agcagatggt caccctggtt gcgagtctgt accagctctt caaacttacg
     6061 gtcagcttcg gcgttagctt ctgcgtcgcg taccattttc tggatttcat cttcgttcag
     6121 accagaagaa gccttgatgg tgatcttctg ctctttaccg ctgtttttat ctttcgcgga
     6181 aacgtgcagg ataccgtcag catcgatatc gaaggtaact tcgatctgcg gcatgccgcg
     6241 cggtgccggg ttgataccat ccaggttgaa ctgacccaga gatttgttat cagccgcacg
     6301 tttacgttca ccctgcagca cgtggatggt taccgcagac tggttgtctt cagcggtaga
     6361 gaacacctgg ctgtgcttgg tcgggatagt ggtgtttttc gcgatcagcg tcgtcatcac
     6421 accgcccatg gtttcgatac ccagagacag cggggtaacg tccagcagca gtacgtcttt
     6481 cacgtcacca gtcagaacac caccctgaac agcagcaccg attgctacag cttcgtccgg
     6541 gttaacgtct ttacgcggct ctttaccaaa gaattcagca actttcttct gaaccattgg
     6601 catacgagtc tgaccaccaa cgaggataac gtcgtcgata tcagatacgg acaggccagc
     6661 gtcctgcagt gcaactttca gcggctcaat ggaacggttt accagatctt caaccaggct
     6721 ttccagtttc gcacgagtca ctttgatgtt catgtgtttc ggaccggtcg cgtctgcagt
     6781 gatgtacggc aggttaacgt cggtctgctg agcggaagac agttcgattt tcgctttttc
     6841 tgccgcttct ttcaggcgct gcattgccag cggatcgttg cgcaggtcaa tgccctgatc
     6901 tttcttgaat tcttcaacca ggtagttgat cagacggctg tcgaagtctt caccacccag
     6961 gtgggtatca ccgttggtag ccagaacttc gaaggttttt tcgccgtcaa cttcgtcgat
     7021 ttcgataatg gaaatatcga aagtaccacc acccaggtca taaaccgcga tagtacggtt
     7081 gccagtacct ttgtccagac cgtaagccag cgcagctgcg gtcggttcgt tgatgatacg
     7141 ttttacttcc agaccagcga tacggcctgc gtctttggtt gcctgacgct gagcatcgtt
     7201 aaagtatgcc ggtacggtaa taacagcttc agttaccggt tcacccaggt aatcttcagc
     7261 ggttttcttc atttttttca gcacttcagc agagatctgc ggcggtgcca ttttctggcc
     7321 tttaacttcg acccatgcgt cgccgttatc agcagcaata attttgaacg gcatgatgga
     7381 aacatcacgc tgtacttctt cgtcctggaa gcggcggcca atcaggcgtt taatcgcaaa
     7441 cagggtgttt tgcgggttcg tcactgcctg acgtttagcc ggctgaccaa ccagagtttc
     7501 accatcctgg gtataggcaa tgatagaagg cgtggtgcga tcgccttcgg cgttctccag
     7561 tacacgagga gtggtgccat ccataatcgc tacacaagag ttggtagtac ccaggtcgat
     7621 accaattatt ttacccatct aaacgtctcc actatatatt cggtcatcat gtggttgtga
     7681 gtctgtaata ggggcgaaac gtctggtttc aactgcccaa tttcattttt ttgcagactc
     7741 actcactgcg gttgactact aaatggggtc gtaacccacg tcatcaaggg ggagatgcaa
     7801 aaattttttg tgcttttgcg cagaaatttc gcgatatggc aggacaagac gtgaaactga
     7861 caacaatttt attcatataa agaatgaatc gcagccaagg cattcatcaa aaaattgtaa
     7921 taaaaagaaa atattacgta cctgaaactt ctctttatca gcagtaaact agtttcatcc
     7981 ccttacccct ccccaccaag agaatcctta tgaaatccgt tattacgatt tccgccagcc
     8041 tggcgattag cctgatgctg tgctgcacgg cgcagccaaa cgaccataaa atcctcggcg
     8101 tcattgcaat gccgcgaaac gaaaccaacg atctggcgct gaaacttcct gtttgtcgca
     8161 ttgttaaacg catacaactc tcagccgacc atggcgattt gcagttaagc ggtgcatcga
     8221 tttatttcaa agccacccga agcgccagtc agaccctgaa tattccttca gaaataaaag
     8281 aagggcaaac cactgactgg atcaacatta acagcgataa cgacaacaaa cgctgcgtct
     8341 caaaaatcac cttttccggt catacagtga actcatcgga tatgaccacg ctgaaaatta
     8401 tcggcgacga ctaacgccag aatatagaaa ccacaaaaaa tgaatgttaa ttacctgaat
     8461 gattcagatc tggattttct ccagcattgt agtgaggaac agttggcaaa tttcgcccgg
     8521 ctgctcaccc ataatgaaaa aggcaaaact cgcctctcca gcgtactgat gcgcaacgaa
     8581 ctgtttaaat cgatggaagg acatcccgag cgacaccgcc gcaactggca gctgattgcc
     8641 ggagaattac agcattttgg tggcgatagt atcgccaaca aactgcgcgg acacggtaaa
     8701 ttgtatcgcg ccattttgct cgatgtttca aagcgattga agctgaaagc cgacaaagag
     8761 atgtctacgt ttgaaattga gcaacagtta ctggaacaat ttctgcgtaa tacctggaag
     8821 aaaatggacg aggaacataa gcaggagttt ctgcacgcgg tcgatgccag ggtgaatgag
     8881 ctggaagagc tgctgccgct gctgatgaaa gacaaattat tggcaaaagg tgtgtcgcat
     8941 ttgctttcca gccaattgac ccgcatttta cgcacccacg cagcaatgag cgtgcttggg
     9001 catggtttgc tgcgcggcgc gggtctggga ggccctgtgg gcgcagcatt aaatggggtt
     9061 aaagcggtca gcggcagtgc ctatcgcgtg acaattccag ccgtactgca aatcgcctgc
     9121 ctgcgccgga tggttagcgc cactcaggtc tgataccgga aaccggggaa aattttcatt
     9181 ttcccctcaa aagatcgtag acactgcccc actggctgat tattatgccg cgccctgaaa
     9241 acactacagt tattcaggga aattatttca ccattcattc gatgatgatt tttgaggaat
     9301 tatgggcaac actaagttgg ctaatccggc accgctgggc ctgatgggct tcggcatgac
     9361 caccattctg cttaacctgc acaacgtggg ttattttgct ctggacggta ttattcttgc
     9421 catgggcatt ttctacggcg gcatcgcgca aatttttgcc ggtctgctgg agtacaaaaa
     9481 aggcaacact ttcggtttaa ccgcattcac ctcttacggt tctttctggc tgacgctggt
     9541 tgcaattctg ctgatgccga aactggggct gaccgatgcg ccaaatgcac agttccttgg
     9601 tgtctacctg ggtctgtggg gcatatttac gctgtttatg ttcttcggca cgctgaaagg
     9661 cgcacgcgtt ctgcaattcg ttttctttag cctgaccgtg ctgtttgccc tgctggcgat
     9721 cggtaacatt gccggtaacg ccgcgatcat ccactttgcc ggctggattg gtcttatctg
     9781 cggtgccagt gcaatctatc tggcgatggg tgaagtgctg aacgagcagt ttggtcgcac
     9841 cgttctgccg attggtgagt ctcactaatt gttaagctcc ccccgctcga agggataaga
     9901 ttgttaacaa cgtgcgcttt gtttatgccg gatgcggcgt gaacgcctta tccggcctac
     9961 aaaatcgtgc aaattcaata tattgcaaga agatcgtagg cctgataagc gtagcgcatc
    10021 aggcagtttt gcgtttgtca tcagcctgat ccccccgcct ggggggattt ttttattcgc
    10081 taacttcgcg tcttgcactc ttcggtctga atgctgcaac cacttccggt gccgtttcaa
    10141 cgtatggccc ttccagcaac tgaatgcagt acggtacgct ggcaaaaata ccgtgcacca
    10201 caacgttacc ctcagcgtcc ttcacacctt ccagcgtctc tttaatagac ttcggttgac
    10261 cgggtaagtt aaggatcagc gcctgtttgc gaatcacccc cacctgacgc gaaaggatcg
    10321 cagttggtac aaaatgcagg ctgatctggc gcatctgttc accaaagcca ggcatctcgc
    10381 ggtccgctac tgccagcgtc gcatcgggcg ttacgtcacg acgcgccggg ccagttccgc
    10441 ccgtggtgag caccagatgg caactcattt catccaccag ctcacacaac gtttgctcga
    10501 tgatcgcctg ctcatcgggg attaagcggg tttccagttc aaacggcgtg gttagcgccg
    10561 atgtcagcca ttcttccagc gcagggatgc ctttatcctg ataaacgccg ctggatgcgc
    10621 gatcagagat ggaaactaag ccaatacgta aagtattcat caagattttt ccgtttaaac
    10681 agtctcgtta aacagaatga tacactgcga agggaatgac agacaggctt cagaagaggt
    10741 agcgtgaccg acttcccggt cacgctaaga atgattacag cagatcgccg atcatttttt
    10801 ccagtttttc ctggtcaaca gcaaacttac ggataccttc cgccagttta tctactgcca
    10861 ttggatcctg gttatgctgc cacaggaact cggactcagt gatacgcgcc ggacgcgctt
    10921 tcacttcgcc ggtgtaagac agtttacgtt cgatagcccc ttcgctctca gccagctctt
    10981 tcagcagcgc cggtgcgatg gtcagacggt cgcagcctgc cagttccaga atttcgccga
    11041 tgttacggaa gcttgcgccc atcaccacgg tttcataacc gtgctctttg tagtactggt
    11101 agatttcaga tacagaaacc acgcccggat cttctgccgg agcgtactct ttcttgtcgg
    11161 tattcgcttt gtaccagtca agaatacggc caacaaacgg cgagatcagg aacacgcccg
    11221 cttccgcaca agcacgagcc tgagcgaagg agaacagcag ggtcaggtta cagttaatac
    11281 cttctttttc cagctgttct gcagcacgga taccctgcca ggtagaagcc agtttgatca
    11341 gaatacgatc gttgctaatc cctgcatcgt tgtagagttt gatcaggcgt tttgctttcg
    11401 caattgacgc ttcggtgtca taggaaagac gcgcatcaac ttcagttgag atacggcccg
    11461 gaaccagttt caggatttcc agaccaatat ttacggccag tttgtcggtc gcgtccacga
    11521 tctgctgcgc gcgatcgttg ctctgctgtt tcgcccaggc gacagcatca tcaatcaact
    11581 tacggtattc cggaatctgc gctgcgttaa gaatgagaga agggttggtt gtggcatcct
    11641 gcggttgata cagcttcatt gccgcgatgt ccccagtgtc ggccactacg gtggtgtact
    11701 gacgaaggga ggtcaatttg tccgtcatga tagtatttct ctttaaacag cttgttaggg
    11761 ggatgtaacc ggtctgccct gatgatatca cgacgcaacg tcagcgcaac cgccgacaag
    11821 acggttatcg ccaggcgaga ctgtttcgga tttctgacac tgcgttgata tcgcccgcca
    11881 tttttataca aaacctcatg tatgctacgc agaagttatc aagtacctcg tagcgtatat
    11941 acttcttaaa caattggtaa cgtttacaca ggaaagtcat cgcgaccggc aataagaggg
    12001 atatgcatgc cagatttttt ctcctttatt aacagcgtcc tttggggatc ggtaatgatt
    12061 tacctgctct tcggcgcagg ttgttggttc acttttcgca ccggatttgt gcagtttcgc
    12121 tacatccgcc agtttggcaa aagtcttaaa aatagcattc atccacagcc aggcggttta
    12181 acctcatttc agtcattgtg taccagtctt gcagcgcgcg tgggtagcgg caacctggcc
    12241 ggcgttgcgc tggcaattac cgccggtgga cctggagccg tcttctggat gtgggttgcc
    12301 gcatttatcg gcatggcgac ctcgtttgcc gaatgttccc tcgcacagct ttataaagaa
    12361 cgtgatgcca atgggcagtt tcgtggcgga ccggcatggt atatggcgcg cgggctgggg
    12421 atgcgctgga tgggcgttct gttcgccgtc tttttgctca tcgcctacgc cataattttc
    12481 agcggagttc aggcgaacgc cgttgcgcga gccctgagtt tttcttttga ttttcccccg
    12541 ctggtaacag gtattattct cgctgtcttt gctctgctgg cgatcactcg cggtcttcat
    12601 ggcgtcgccc ggctcatgca ggggtttgtc ccgttgatgg cgataatctg ggtactgacc
    12661 agcctggtga tttgcgtaat gaatatcggg caacttcccc acgtcatttg gtctattttt
    12721 gagagtgctt ttggctggca ggaagctgca ggcggcgcgg cgggatatac cttaagccag
    12781 gcgattacta acggttttca gcgcagtatg ttttccaatg aggcgggaat ggggtcaacg
    12841 ccaaacgcgg cagcggcagc tgcgtcctgg cctccgcatc cggcagcgca aggaattgtc
    12901 cagatgattg gcatttttat cgacaccctg gtcatctgca cggcaagcgc catgctgata
    12961 ttactggcgg gtaacggcac aacctacatg ccgctggaag gtattcagct tatccagaag
    13021 gcgatgcggg tgctaatggg ttcctggggt gcggagtttg ttaccctcgt ggttattctg
    13081 tttgccttca gctccatcgt tgccaactac atttatgccg aaaacaatct cttcttttta
    13141 cgcctgaaca accctaaagc gatctggtgt ctgcggatct gcaccttcgc aacggtcatc
    13201 ggcggcacct tgctaagttt tccgctgatg tggcaactgg cagatatcat tatggcctgc
    13261 atggctatta ccaatttgac cgccatatta ctgctctcgc ctgtggttca taccattgcc
    13321 agtgattatc tacgccagcg taaactcggc gtgcgcccgg tgtttgatcc gttgcgttat
    13381 ccggagatcg gtcgccagct ttctccagac gcgtgggatg atgtttcgca ggagtaatca
    13441 caactatcga tcaactcatt ctcttttttt gctaaagtcg gcataaattt cctgcaagga
    13501 ctggatatgc tgattcttat ttcacctgcg aaaacgcttg attaccaaag cccgttgacc
    13561 accacgcgct atacgctgcc ggagctgtta gacaactccc agcagctgat ccatgaagcg
    13621 cggaaactga cgcccccgca gattagctcc ctgatgcgca tcagcgacaa actggcgggt
    13681 atcaacgccg ctcgctttca tgactggcag ccagatttca caccggagaa tgcccgccag
    13741 gcgattctgg cgtttaaagg tgatgtctac accggcttgc aggccgaaac cttcagcgaa
    13801 gacgatttcg attttgccca acagcatttg cgaatgcttt ccggcttgta tggcgtactc
    13861 cgcccgctcg atttaatgca gccttatcgt ctggaaatgg ggatccgtct tgagaatgcc
    13921 cgagggaaag atctgtatca attctgggga gatatcatca ccaacaagct gaacgaggcg
    13981 ctcgcagcac aaggcgataa tgtggtgatt aatctggcgt cagatgagta ttttaaatcg
    14041 gtgaagccga agaaattgaa tgccgagatt atcaagccgg tgttcctcga tgagaagaac
    14101 ggcaaattta agatcatcag cttctacgct aagaaagcac gcggtctgat gagtcgtttc
    14161 attattgaaa atcggctgac caaaccagag caactgactg gttttaatag cgaaggttac
    14221 ttctttgatg aagcttcctc cagcaatggc gaactggtgt ttaaacgcta cgagcagcgt
    14281 taagtttgta gtcaatgccg gataatgccg gatggcaatc cggcattgat atttagcgat
    14341 gatgtttgtc aggccccgga cgatgatcgc cacgatgatc attgtgcttg ttatggcgcg
    14401 gcgatggcgg cggtccatgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc
    14461 accagccgtg gtcgcgccag tgaccgccat cccagtaata gccacgatta tcacgatcgc
    14521 ctatctgtaa ttttactgac gggactaacg taatttccgc agcctgcgct gccatgggag
    14581 cgaccagaac cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct
    14641 tttatcattg cccgttatgg gtcgatatcc ggagattacg cgatgagaaa ggcggttgtt
    14701 attctccgca atcctaatct ctaagttacc gcatttattg agaatttctc ctttttccct
    14761 gtcgtttctc tccatatttt cttcacaaaa aagccgggct gcataaaagc aaacccggcc
    14821 tgattgagat aatgaataga tgttactgat gattcatcat caatttacgc aacgcagcaa
    14881 aatcggcggg cagattatgt gaaagcaagg gtaaatcagc tcgttctgcc agctcttttg
    14941 gcagatccaa cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg
    15001 cggtgccgag gaacaagcca tattcgcctg gattcaactg gtcacgcagc gcacgatacg
    15061 ctacggcagc gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt
    15121 gcgtggtttc atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc
    15181 ggaacaactc ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata
    15241 acgtcgcctg agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat
    15301 cgttcacgtt ggtcgcagca ataaaacgtt tcaccggcag accgagtgac ttcgccagca
    15361 gacccgccgt caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg
    15421 cttcctgcgg cagctgcgca actgcttcaa agtagtagca aatctgcgcc agcaaacggc
    15481 tgatgttaat cgagttagct gagtttaacc ccagcgccac tttcagctct tcatcatcaa
    15541 acgcctgctt caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga
    15601 tattgccgcc caatgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat
    15661 agaggataac cactttcaca ttcggtaaac cgtagaaagc atgagccact gccgctccag
    15721 tatcaccgga tgtcgcggtc agaatggtca ctggcttatc gcccgcaata tgggtcagca
    15781 tttgtgccat aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt
    15841 ccagacaacc gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcac
    15901 gtacgcgctc ttccaggatt tcctgcggga tttcatcacc aataaacgcc gagaggatct
    15961 tcgcactgcg ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgtatt
    16021 ccggcaggtc gtgcggaaaa aacagcccct gatttttgcc taacccctgg gttacggctt
    16081 gcgcaaagct gacctgctca ttgtgatctt tcagattgta gagtttcatt tagttttcca
    16141 gtactcgtgc gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca
    16201 ggtagttctt acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacacagag
    16261 cgaacaaagt cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcaaccg
    16321 cctgccgcgc ctgccggaag cctggcagta accgttcacg gtagggttca gcgataacat
    16381 ctttcatcag cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagcctg
    16441 ccagatgtcg cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc
    16501 tggcttctgc cgtcgagact ttaatccccg gatacgccag cacccacagc cactcatcaa
    16561 accctggcac ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa
    16621 gaaaacacgg tgccacgttg tcgtaatgaa tgctgccgga gatacgccct tccaactcgc
    16681 ccatcaaagc cagcaaacga gtgtcattaa gcggcttgcc gcagtgttca ttcatcgcca
    16741 tcagcgccgc gaccactgaa caggcgctgg agcctaagcc cgaaccgatc ggcatattct
    16801 tttccagagt catcgccact ggaatttgct tgccaagctc ctggcaaaaa cgctcccagc
    16861 actgataaac gatattttcc cgtggctctg acggcagctt atcggcaaag cgtccgaggt
    16921 tgttgagact gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat
    16981 caacgggtgt caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag
    17041 ccggggcata aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga
    17101 tcagcaaaga caccggcagc tgtaacgtca ttgcccgcac catatccgcg cagtaccaac
    17161 ggcagcggct gataatagtg gctatagaag gccagggcgt tttcgccatt tttcactttg
    17221 aacagcggat cattaccatc cacttcggca atcttcacgc ggcagacgcc atcttcatca
    17281 atattgccaa catagcgcaa aacttttcct tcatcacggg ccttcgccac acgcgcggca
    17341 aagagatcgt cgagctgtga cagattcgcc ataaaagcgg cgacatcacc ctcggcgtta
    17401 aactctgcgg gcagcacagg ttcaatttca atatccgcca gctccagttc acgtcccgtt
    17461 tcacgagcga gaatcaatag cttacgcgcc acatccatac cagaaagatc atctcgcggg
    17521 tccggttcgg tataacccat ttcccgcgcc agtgtggtcg cctcggagaa actcatgcct
    17581 tcgtctaact tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcatc
    17641 aattcatcac cagcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca
    17701 acgttggtgt catagaggaa tttacgccgc gatttttccg ccgcataacg caactgatgg
    17761 tagtaatcca tcgacgaggt gttggccttt ttgttcggcg taacaacgtg gaaaccttcg
    17821 cgcaggaagt cggcatattg atccgccact gcctggctgg aagtacagtc aacaatgacc
    17881 gggttcagca gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct
    17941 ttggcttgcg ccagttcttc ctgccagttt tccagattaa ggccatgtac attggtgagc
    18001 agtgccttcg agttagcaac accgcagaca cgtaagtcga tatgtttatt cttcaaccag
    18061 ctttgctgac gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca
    18121 aacacttcga taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg
    18181 gtcgcatcat cgttattgac cacgacagag attgagcgtt cagaagatcc ctgagcaatg
    18241 gcgacaatgt tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgt
    18301 aaggtgcgca taccatcacc taccaccgag ataatggcca gccgttccgt caccgccaac
    18361 ggctccagta agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagct
    18421 cgcacacagt cgctttgcgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta
    18481 atcagcacca cggaaatacg ggcgcgtgac atcgctgcaa agacgcgcgc cgccatgcca
    18541 accatccctt tcatccccgg gccggaaacg ctgaacattg ccatgttatt cagattggaa
    18601 atgcccttga ccggtaattc gtcttcatca cggctggcac caatgagcgt acctggtgct
    18661 tgaggatttc cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg
    18721 gtgcgggggt gaagaacttt agcgccgaag taagaaagct ccatcgcttc ctgataggac
    18781 atcgacttca acaacctcgc atcgggcacc tgacgcggat cgcaggtata aacaccgtca
    18841 acatccgtcc agatctcgca acaatcggcg cgtaaacagg ccgccagcac cgcagcggag
    18901 tagtcggaac cgttgcgtcc cagaaccacc agctcgcctt tttcattacc ggcagtgaaa
    18961 ccagccatca gcaccatgtg gtcagccgga atgcggcttg ccgcaatacg gcgggtggat
    19021 tcagcaatat caacggtaga ttcgaggtaa tgacccactg ccagcagttt ttcgaccgga
    19081 tcgataacgg taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac
    19141 attttctcgc cacggcaaat cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa
    19201 ctgatgccat gcaggacatg ttttatttgg gcaaattcct ggtcgacgaa agttttcaat
    19261 tgtgccagcg gaaatcccgg ctgggcggcg gcgagtcccg tcagaagttc ggcaaaaata
    19321 cgttcggcat cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc
    19381 gctaccagat ggttggtaat tttggcgggg gcagagagga cggtcgccac ctgcccctgc
    19441 ctggcattgc tttccagaat atcggcaacc cgcagaaaac gttctgcatt tgccactgat
    19501 gtaccgccga acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa
    19561 gcccgcactg tcaggtgcgg gcttttttct gtgtttcctg tacgcgtcag cccgcaccgt
    19621 tacctgtggt aatggtgatg gtggtggtaa tggtggtgct aatgcgtttc atggatgttg
    19681 tgtactctgt aatttttatc tgtctgtgcg ctatgcctat attggttaaa gtatttagtg
    19741 acctaagtca ataaaatttt aatttactca cggcaggtaa ccagttcaga agctgctatc
    19801 agacactctt tttttaatcc acacagagac atattgcccg ttgcagtcag aatgaaaagc
    19861 tgaaaaatac ttactaaggc gttttttatt tggtgatatt tttttcaata tcatgcagca
    19921 aacggtgcaa cattgccgtg tctcgttgct ctaaaagccc cagccgttgt tgtaaccagt
    19981 cgaccagttt tatgtcatct gccactgcca gagtcgtcag caatgccatg actcgttcgc
    20041 gtaaagcatg cagttgatgt tggtctgccg ttgtgtcact tttcgccggt tgttgtatta
    20101 atgttgctaa ttgatagcaa tagaccatca ccgcctgccc caggttgagc gaaggataat
    20161 ccgccaccat cggcacacca gtaagaacgt cagccaacgc taactcttcg ttagtcagcc
    20221 cggaatcttc gcgaccaaac accagagcgg catggctcat ccatgaagat ttttcctcta
    20281 acagcggcac cagttctact ggcgtagcgt agtaatgata tttcgcccga ctgcgcgcag
    20341 tggtggcgac agtgaaatcg acatcgtgta acgattcagc tagtgtcttg aaaactttaa
    20401 tattatcaat aatatcacca gatccatgtg cgacccaacg ggcggctggc tccaggtgtg
    20461 cctgactatc aacaatccgc agttcgctaa aacccatcgt tttcattgcc cgcgccgctg
    20521 ccccaatatt ttctgctctg gcgggtgcaa ccagaataat cgttatacgc atattgccac
    20581 tcttcttgac caaataaccg cgaacagggt gatcattgtc aacttattac gcgttgcgaa
    20641 tttacaaatt cttaacgtaa gtcgcagaaa aagccctttg cttagcttaa aaaaggctaa
    20701 actatttcct gactgtacta acgattgaat tgtt
//
LOCUS       DAZFJL010000084        20475 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFJL010000084 DAZFJL010000000
VERSION     DAZFJL010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.084"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(129..1232)
                     /gene="gldA"
                     /locus_tag="ACHJ8C_002998"
     CDS             complement(129..1232)
                     /gene="gldA"
                     /locus_tag="ACHJ8C_002998"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO5552819.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(1243..1905)
                     /gene="fsa"
                     /locus_tag="ACHJ8C_002999"
     CDS             complement(1243..1905)
                     /gene="fsa"
                     /locus_tag="ACHJ8C_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO5552820.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVKEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(1917..4418)
                     /gene="ptsP"
                     /locus_tag="ACHJ8C_003000"
     CDS             complement(1917..4418)
                     /gene="ptsP"
                     /locus_tag="ACHJ8C_003000"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO5552821.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPISLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPTAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVRAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGESVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            4727..5806
                     /locus_tag="ACHJ8C_003001"
     CDS             4727..5806
                     /locus_tag="ACHJ8C_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO5552822.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGVMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTEEKEDDLDLDFEIN"
     gene            5821..6141
                     /locus_tag="ACHJ8C_003002"
     CDS             5821..6141
                     /locus_tag="ACHJ8C_003002"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO5552823.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            6192..8489
                     /locus_tag="ACHJ8C_003003"
     CDS             6192..8489
                     /locus_tag="ACHJ8C_003003"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO5552824.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVIMRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DGQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQTSLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ESGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFAMPAGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            8455..9333
                     /locus_tag="ACHJ8C_003004"
     CDS             8455..9333
                     /locus_tag="ACHJ8C_003004"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO5552825.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDAAQARDVVKMNLQRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            9335..9676
                     /locus_tag="ACHJ8C_003005"
     CDS             9335..9676
                     /locus_tag="ACHJ8C_003005"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO5552826.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(9663..10514)
                     /locus_tag="ACHJ8C_003006"
     CDS             complement(9663..10514)
                     /locus_tag="ACHJ8C_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO5552827.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFIDTGAGAALVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQSQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10740..12473)
                     /locus_tag="ACHJ8C_003007"
     CDS             complement(10740..12473)
                     /locus_tag="ACHJ8C_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO5552828.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLGNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPEDQGKFDGNTEHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAT
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(12656..15307)
                     /gene="ppc"
                     /locus_tag="ACHJ8C_003008"
     CDS             complement(12656..15307)
                     /gene="ppc"
                     /locus_tag="ACHJ8C_003008"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO5552829.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(15609..16760)
                     /gene="argE"
                     /locus_tag="ACHJ8C_003009"
     CDS             complement(15609..16760)
                     /gene="argE"
                     /locus_tag="ACHJ8C_003009"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO5552830.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLAKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYDAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHKLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            16914..17918
                     /gene="argC"
                     /locus_tag="ACHJ8C_003010"
     CDS             16914..17918
                     /gene="argC"
                     /locus_tag="ACHJ8C_003010"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO5552831.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDVAFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVSQVQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            17926..18702
                     /gene="argB"
                     /locus_tag="ACHJ8C_003011"
     CDS             17926..18702
                     /gene="argB"
                     /locus_tag="ACHJ8C_003011"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO5552832.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRQLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTNEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            18763..20136
                     /gene="argH"
                     /locus_tag="ACHJ8C_003012"
     CDS             18763..20136
                     /gene="argH"
                     /locus_tag="ACHJ8C_003012"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO5552833.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYHLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKALEDLPLSELQKFSQVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
BASE COUNT         4669 a         5196 c         5665 g         4945 t
ORIGIN      
        1 aacgccttat ccggcctaca aaagtacgta aatttactat attgcagaga ttgcgtaggc
       61 ctgataagcg tagcgcatta ggcaattttg cgttcaaact cccggacaag ccgggagttt
      121 ggagtaggtt attcccactc ttgcaggaaa cgctgaccat actggtcggc taccagcagt
      181 gcggcgtaaa cctgatctgg cgtcgcgccg ccaggcatgt tgtggatggt ttcaccttct
      241 gcacatgccg cttctgccac aattcgcatt ttcgccggga catcttcttt aatatccagt
      301 tgagcgagag ttattggcaa acctaccgca tggctaagcg cagctacggt ttcgatttcc
      361 tccaccggcg cgttttccag aaccagctgc gtcagcgtac cgaatgccac tttttcacca
      421 tgataatagt gatgcgcgtc cgggatagcg gtcaggccgt tatgtaccgc gtgtgccgcc
      481 gccagcccgc cgctttcaaa accaacgcca ctcaaatagg tgttcgcttc aatcacgcgc
      541 tccagcgccg gagtcactac atgctgttcg gcggcaagca tcgctttttc gccttcttcc
      601 agcagggtgt tgtagcacag ttcagccagt gccagcgctg cctgagtgca cttgccaccc
      661 gccatggtgg tcgcgccgct acgggaacag gcacgcgctt caaaccaggt tgccagcgca
      721 tcgccgatac ccgccgctaa cagacgtgca ggtgcgccag cgacgatttt ggtgtcgaca
      781 atgaccatat tcgggttatt tggcaacagc agatagcggt caaactcacc ctcatcggtg
      841 tagataacag acaatgcgct gcacggtgcg tcggtagaag cgatagtcgg tgcgatcgct
      901 accggaacac ccatgaaatg tgccagtgct ttggcagtat cgagggtttt tccgccaccg
      961 ataccgagaa ttgcgccaca ctgcgcagtc tccgcgatgc cacgcagacg gtcgatctca
     1021 ttttgcgaac attcaccgcc aaacggcgca atttctacta ctagtccagc atctttaaag
     1081 cttttctcga cagtggattg agcaaaacct aaaacaaatt tgtcacccac cactaaccag
     1141 cgttccgcca gtggcttcag gtactcgccc agacgattaa ttacatctgc gccctggatg
     1201 tatttacctg gtgattgaat aatgcggtcc ataattactc ctttagagat gagtagtgcc
     1261 aaatgcggca ttccagtcgt gctcgaactt ctctatagct gactctaccg caggggtgtt
     1321 aagcatttgt tgcgctacat ctaagggcag tgtgatggat tcacatccag ccagcaaaca
     1381 atccagcgcc tgacgtggtg ttttaaagct ggcagccagc accatgcttt ctggcgcatg
     1441 catttccaac agcgcttgca gctcctgtac agtacgaatg ccgtcaccgc cctgggcatc
     1501 tacgcggtta acgtatggag caacgtattt ggctccagcc agcgccgcca gtaatccttg
     1561 cgcggcgctg tacactgcgg ttcccagcgt agtaatgcct tctttcttca gcattttaat
     1621 tgctgccaga ccttcagagg ttaccggaat tttaaccaca atgcccggaa tagcgtcgcg
     1681 caggcgtttc gcttctttca ccataccctg cgcgtcgcgg ctcatggtct gagcaaacag
     1741 aattccctca tcaccgatcg ctttttgaag gcgcggcagc acttcccaga tggactcctt
     1801 gctggcagcg ataatgctcg ggttagttgt caccccggca atcgggaata tgcgtgccag
     1861 acgttcgact tctgcgacgt tagcggtgtc cagatacagt tccatgatgt ttccttttac
     1921 agttccagtt catgttgcag caggctggcg atagcgtcgg cagaggcggc gttaaccagc
     1981 gcattacgga attcttcgtg catgatgcga cgagcgaggc gcgagaaaat gcgcatatgt
     2041 tgatcgcccg cggcgtgttt gttcagggtt aacataatga tgaattgcgc ttcatcatcg
     2101 ccccagcgca ccggcgcttg caaacgcgct acgctgatgg tggattgctc aatgtgttct
     2161 gatttgctgt ggggaatggc aaaactaaag cccagaccgg tagagaaaac ggcctcgcgc
     2221 gcccacaagt cggcttccag tttacgcgga tagcgacagc ggcccgccag cagcaggtta
     2281 tcggtcatgc ctttgagcac ttcttctttg ctgcgccagt cgctttccag tgtgatgcac
     2341 tcggcggtga ccagcggtgc gtcttgttgg gtcatgcgga attgcgccag caggtgttcc
     2401 acttccagcg aagtacggca ggccattgcc tggttgagca acttgcggca ctcacggcta
     2461 tcaagttgcg ccatccgcgc tttcgccgcc ggaattgatg gtgcgctcat gctgagttca
     2521 tccagcccta aaccgaccag caacggcagc acggaacctt tcgctcccag ttcaccgcac
     2581 agaccaatcc atttgccctg gcgatgcacc gcctgcacgg cgtaatcgag cgcccgcaag
     2641 aaggccggat tcaggctgtt gtagtgacga gtcaccttag cgttatcgcg atccactgcc
     2701 agtagatatt gcgtcaggtc attactacca atactaaaga aatcaatctc ttcgcagcat
     2761 tgatcgatga tgaacatcac cgacggcact tccagcataa tgccgagctg gatcttctca
     2821 tcaaacggaa tgtgttcgtt acgtagttgc tgtttggctt ctgccagttt ttcttttacc
     2881 cataagatct cttccattga ggagatcatc gggatcatga ttttcaggct gccgtgagca
     2941 gaggcgcgca ggatcgaccg tagttgtgtg gtaaacaacg acgcgtattc ttcataaata
     3001 cgcaccgcgc gataaccgag gaacgggttt gcctctgcgg gaatattcag ataatcaacg
     3061 ggtttgtcac cgccaatatc catagtgcgc acaataatgc tgcgtccgtt ggcggactcc
     3121 agcgcctgac aaaaaatgtt gtacagctcg ctttcgcccg gtgcactggt gcgatccata
     3181 tagagcattt cagtgcggaa taaaccaacg ccttccgcac cattgccgaa tgcggcctgc
     3241 gcttccacgg agtgagcgat gttagcggca atttcaatgc ggataccgtc agcggtacgc
     3301 gcttgttggg tcagccagac acgctgttgc tcacgcagag cgtcctgtac gcgggcttct
     3361 tgctgataat aacgggccac ggattcccct ggctcaacca caatcgcccc tgcgttgccg
     3421 tcgatataaa tcgtttgatg ttgccacgga gtaagggcat caatatccac acctaccagc
     3481 gtcggaatgt tgaacgaacg ggcaaggatc accgtatgtg aggtggtgcc gccacttttc
     3541 agtaatagac ctttgaggtg atttttatcc agttcgagga actggctggg ggtcagttca
     3601 tcagccatac aaatggcggg ctgcgtcagt ttgcccggtg ccgggaagcg ttgctcaccg
     3661 tagatttgct ggagtaactg gaagcagacg tcgcgtacgt ccagggcacg ttcttgcagg
     3721 taactgctgc tggaacgggc gaactcttcg caaaagtgat tcgcactggc aacaatcgct
     3781 tcggcgcagc ttaatccggc cctgacacct gccagtaaat gttcgcgcag ggaagtatcg
     3841 ccagccaggg atcggtgcgc ttccagaatc gcgctggtag caccgtcgct atccagcaga
     3901 cgaaactcta tgtttttcag taccagcgtc agaccgtttt ccagtgcgga ttgttcggcg
     3961 tcaacgcctt tggctgtggg aagattaccc agcgcattga gatctaaaga agagatcggc
     4021 gtcagaatgc cgcctgcgct accgctgcac acggtgcgag cacggataat ttgcggattc
     4081 agattggtca gtgaaatcgg cagtggttcc agttcgtcag attttacttc cgccagcggt
     4141 gcgtcgcagt gggggaattc atcgcgcagc cactggctta aacgctggtg cgcttcctgt
     4201 tcgtcggccc cggaaatcag taactggcag ttatcgccca ccagcgtatc ggtgccaatc
     4261 agcgcaaggg cgcttttggc gttgcccttg cggtcagtgc gcaggttatg ccactcaatt
     4321 tgtgatgaaa aagtattaca cagcgtttca acgtggcttg ccggacgcgc atgcacgccg
     4381 ttaggtagct cacaaataaa ttccacaatc agggccattg cctttctccc ggtacggttt
     4441 tctgtttaca ggataagagc gcacggcaac ggcctgccat gtgacaaatc tgccaaaagc
     4501 tggacaaatg taatgtaacc gtcaatttgc gacgcgtctc acaagacgcc gttttgcggc
     4561 gtacttccgg attatcgcaa gttatgaggc ggatcgcatt tttgtactga tattacaaaa
     4621 gtccagtaaa tggccttttt atccactgtt tgctccgcct gcgattgcct attgtttctt
     4681 ccatcaaaaa tatggacatg ggctttcgca agcccgggag cagcttatga atgagttggt
     4741 gcagatcctg aaaaataccc gtcagcattt aatgacgggc gtttcacaca tgattccctt
     4801 cgtggtatcg ggcggtattt tgctggcggt ttccgtcatg ttgtatggca aaggcgcagt
     4861 gccggatgcc gtagccgatc cgaatctgaa aaaactgttt gatatcggcg ttgcaggttt
     4921 gacgctgatg gtgcctttcc tcgccgctta catcggttat tccattgcag aacgttctgc
     4981 gctggctcca tgcgctatcg gtgcctgggt tggtaacagc tttggcgcgg gcttctttgg
     5041 tgcgctgatc gccgggatta tcggcggcat cgtggtgcat tacctgaaga aaattccagt
     5101 gcataaagtt ctgcgctcgg taatgccaat cttcatcatt ccaatcgtcg gcacactgat
     5161 taccgctggc gtcatgatgt gggggctggg cgagccggtg ggggcgttga ccaacagcct
     5221 gacccagtgg cttcagggga tgcagcaggg cagcattgtt atgctggcgg tgatcatggg
     5281 tctgatgctg gcgttcgata tgggcggccc ggttaacaaa gtggcctatg ccttcatgct
     5341 gatttgcgtt gctcagggtg tttataccgt ggtggctatt gccgctgttg ggatttgtat
     5401 cccgccgctg gggatggggc tggcgacgct gattggtcgt aaaaatttct ccgcagaaga
     5461 gcgcgaaacc ggcaaggcgg cactggtgat gggctgtgtt ggcgttactg aaggggcgat
     5521 tcctttcgcc gcagccgatc cgttgcgtgt cattccttcc atcatggtcg gttctgtttg
     5581 tggtgcggta actgcggcgc tggtcggtgc gcagtgctat gcaggctggg gtggtctgat
     5641 tgtgctgccg gtggttgaag gtaagttggg ttacatcgcg gcagtggctg tcggggcggt
     5701 tgtgacggca gtctgcgtga acgtgctgaa aagtctggcg cgtaaaaatg ggtcttcaac
     5761 tgaagagaag gaagacgacc tggatctgga ttttgaaatt aattaattga ggaacgagcc
     5821 atgacgaaaa ttatcgcagt aaccgcatgt ccttcaggtg ttgcccatac ttatatggcg
     5881 gcagaggcgc tggaaagcgc cgcgaaagcg aaaggctggg aagtgaaagt agaaacccag
     5941 gggtcgattg gtctggaaaa tgaactgact gcggaagacg tggcaagcgc cgatatggtg
     6001 attttgacca aagatatcgg catcaaattt gaagagcgtt ttgctggcaa gactatcgtg
     6061 cgtgtcaaca tcagcgacgc agtaaaacgt gctgacgcca ttatgagcaa aattgaagct
     6121 cacctggcac aaaccgctta agttcttatc ccgcccgcaa gggcgggttc gctttcccac
     6181 aggagttcct catgacgaat cgtatctctc gcctcaaaac tgcactgttt gccaataccc
     6241 gtgaaatctc gctggagcgg gcgttgcttt ataccgccag tcatcggcaa accgaagggg
     6301 agccggtgat aatgcgtcgg gcgaaagcaa cagcgtatat ccttgaacac gttgaaattt
     6361 cgattcgtga tgaagaactg attgccggta accgcaccgt aaaaccacgc gccgggatta
     6421 tgtcgccgga aatggaccct tactggctgc tgaaagagct ggatcaattc ccgacgcgtc
     6481 cgcaggaccg ctttgctatc agcgaagaag ataaacgtat ctaccgcgaa gagttgttcc
     6541 cgtactggga aaaacgttcg atgaaagatt tcatcaacgg gcagatgacg gatgaagtaa
     6601 aagccgcgac cagcacgcag attttcagca tcaaccagac ggataaaggc caggggcaca
     6661 ttattattga ttacccgcgc ctgctgaatc acgggctggg ggagctggtg gcacagatgc
     6721 agcaacattg tcagcaacag ccggagaatc acttttatca ggcagcgctg ttactgctgg
     6781 aagcctcgca gaaacatatt ttgcgttacg ccgaactggc ggaaacgatg gcggcaagct
     6841 gcactgatgg ccagcgtcgc gaagaactgc tgaccattgc ggagatctcc cgccataacg
     6901 ccgaacataa gccgcagacg ttctggcagg cgtgccagtt attctggtac atgaacatca
     6961 ttctgcaata cgaatccaac gccagttcgc tatcgttggg gcgcttcgac cagtatatgt
     7021 tgccgttcta tcagacatca ttaacccagg gcgaagatcc ggcattcctg aaagaactgc
     7081 tcgaatcttt atgggtgaaa tgcaacgaca tcgtgctgtt gcgctccacc agtagcgcgc
     7141 gttatttcgc cggtttccct accggctata ccgcactgct cggcgggtta accgagagcg
     7201 gacgtagcgc ggtgaacgtg ctttcgttcc tttgccttga cgcttatcaa agcgtgcaat
     7261 taccgcaacc gaacctcggc gtgcgcacta acgccttgat cgacactcct ttcctgatga
     7321 aaaccgcaga aaccattcgc ctcggcaccg gtattccgca aatctttaac gatgaagtgg
     7381 tagtgccagc gttcctcaac cgtggcgttt cgctggaaga tgcgcgcgac tattccgtag
     7441 tgggctgtgt ggaattatct attcccggca gaacctacgg cttgcatgac atcgcgatgt
     7501 ttaacctgct gaaagtaatg gaaatctgcc tgcatgaaaa tgaaggcaat gctgcgctga
     7561 cttatgaagg tttactggag cagatccgcg ccaagatcag ccactacatc accctgatgg
     7621 ttgaaggcag caatatttgt gatatcggtc accgtgactg ggctcctgta ccgctgctct
     7681 cgtcgtttat cagcgattgt ctggaaaaag gccgcgatat taccgatggc ggcgcgcgtt
     7741 ataacttctc cggcgtacag gggatcggta tcgccaacct gagcgattct ctccatgcgt
     7801 tgaaagggat ggtttttgag caacagcgtt taagttttga cgaattgctg tcggtattaa
     7861 aagccaactt cgcaatgcca gcaggcgaaa aagtccgcgc tcgcttaatt aaccgctttg
     7921 agaaatatgg taacgatatc gacgaggtgg ataacattag cgctgaactc ctgcgtcatt
     7981 actgcaaaga agtggaaaaa taccagaacc cgcgcggtgg ctacttcacg ccgggatcgt
     8041 ataccgtttc tgctcacgtc ccgttgggat cggtggttgg tgcgacgcca gacggccgtt
     8101 ttgccggaga acagctggca gacggcggct tgtcacccat gctgggccag gacgcacaag
     8161 ggccaacagc ggtactgaag tcagtcagta agctcgataa tacgctgctg tctaacggta
     8221 cgttgctgaa cgtgaaattc actccggcga ccctggaagg tgaagcggga ttacgcaaac
     8281 tggccgactt cttacgggcg tttacccagc ttaagttaca acatattcag tttaacgtgg
     8341 tgaacgccga cacgttgcgg gaagcgcaac agcgcccaca agattatgcc gggctggtgg
     8401 tgcgcgttgc cggatacagc gccttctttg tcgaactgtc gaaggagatc caggatgaca
     8461 tcatccgccg gacagcgcat cagctgtaac gttgtggaaa cgcgccgcga tgatgtggcg
     8521 cgcattttca acattcagcg ttattcactg aatgacggcg agggcattcg tacggtggtc
     8581 ttttttaaag gctgtccgca tctttgccca tggtgtgcta atccggagtc gatctccggc
     8641 aaaatccaga cggtgcgcag agaggcgaaa tgtctgcact gtgcgaaatg tttgcgtgat
     8701 gcggatgaat gcccctccgg ggcgtttgaa cggattggtc gcgatatcag ccttgacgcg
     8761 ctggaacggg aagtgatgaa agatgacatt ttctttcgca cgtccggcgg tggcgtcacg
     8821 ctttctggcg gcgaagtgtt aatgcaggcg gagtttgcga cccgtttttt acagcgactg
     8881 cggctatggg gtgtgtcatg cgccatcgaa actgccggag acgcgccagc cagcaagctg
     8941 ttaccgctgg cgaaattgtg cgatgaagtg ttgttcgatt taaaaattat ggatgcggct
     9001 caggcgcggg atgtggtgaa gatgaacctg caacgcgtgc tggagaatct gcgtttgctg
     9061 gtgagtgagg gtgtcaacgt gatcccgcgt ttaccgctga tccctggttt cacgctcagc
     9121 cgggagaata tgcagcaggc gctggacgtg ctgatcccgc tgaatatcag gcagatccat
     9181 ctgttaccgt ttcatcagta cggcgagccg aaataccgcc tgctggggaa aacatggtcg
     9241 atgaaagagg ttgctgcgcc gtcgtcagcc gatgtggcaa cgatgcgtga aatggcagaa
     9301 cgggccggat ttcaggttac cgtgggaggt taaaatggca tatctggtgg cagtaaccgc
     9361 ctgcgtaagc ggcgtggcgc atacttatat ggcggcggaa cggctggaaa agttgtgcca
     9421 gttagagaag tggggcgtca gcattgaaac tcagggcgcg ttgggaacgg agaatcgttt
     9481 agcggacgag gatatccgtc gggcggatgt tgctctgttg attacggata tcgagcttgc
     9541 cggtgccgag cgatttgaac attgccgcta cgtgcaatgc agcatctacg ctttcctgcg
     9601 tgagccgcag cgggtgatga gcgcggtacg caaagtgctt tctgcgccgc agcaaaccca
     9661 tcttattctg gagtagtcgg tttttctgtc agttggctgt ggtactgccg acgatattcc
     9721 gacggtgagc gttcggtatt tttacgaaac agacggcaga agtagttgct gtcgacaaag
     9781 ccgcaggcgt gcgccacttc ttttaccttc aggtcatagc ctttcaataa cgtcttagcg
     9841 tgctccagtc gcgtgtgatt caggtattcg ttaaaaccaa tggcccccgt tttttgaaac
     9901 aggtgcgaga ggtaatttgg cgaaatatag aatgcctgtg cgacagattc gcgggtaagt
     9961 gcggaggcat agcgttcgtc gatataatca cgaatagctt cgaatagagc ctggctgcgt
    10021 gaggcggtct gtatttggct gccgagcaaa tcgcggcagt ggctaagcag gctggcgacg
    10081 ataagtctgg ccgtttgctg ctcctgcgac tgcatttgca tttcattgag cgtttgtagc
    10141 agaaaagaac caatgcgcgg gccgcgtcgg gcgacgtgtt gcttcgccag attttgatat
    10201 tgtttgccat cccattgcac aacgctgaag ccgagttgct gtttgccaaa cagcacgcta
    10261 aaggtggtgg cgggggcttg ccattgtgga aaattccagc caccagcagg gacgtacagc
    10321 acatcgccgg gaactaacgc tgctccagcg ccggtatcaa taaactcacc ttccagcaca
    10381 atttccagcc gtggaaaatc gacctgatac gcaagatcgg gcaccgggcc gttgctgctg
    10441 gcaaagtaaa tctggcgcag ggacagcggg ccgttgatca ggcgggagag cagatagctg
    10501 acgtcgtgat acatgttatt cccttaggta tgtagacggg aaaacagtaa ctggcattat
    10561 gaaaaaaggc cacctgaaaa gtgtggcctg atgggcgtga gatgtaaaca ttgcggcagt
    10621 ttcagtcgac ttgtattgcc ggatgcggcg taaacgcctt atccggccta ccgccgcgat
    10681 ctgtaggccg gataagacgc gtcaagcgtc gcatccggca aataacatct ggctgataat
    10741 tactgattac ccacctgatc gccatacggc agagtgtcgt aatcgatcag tgcgtttttc
    10801 ttatacgggt taccaatcca gcgggtagtt tctttgaact gcggattcac cactgaacgg
    10861 gttggatcgt aaccgtcgta agataagccc gccaaatctg accaggtgtg gatcagttcc
    10921 gccaggctgt atttacgatc aacatcctgt gagaaatcac gcggatgagt cgcttgccat
    10981 ttttctgacg tccacagcag gaacggaatg gtgtacatat gacgcgtcgg gttgtcttca
    11041 ttacgcccct gagttttgtg cggcggcgtg tcgtaaacct cctcaccgtg gtcagagaaa
    11101 taaaccagga aaccgttcgg atcggtcgct ttaaagtctt taatcaggct ggcaaccacg
    11161 tgatcgttgt acaggttagc attgtcataa tcattatatg actccagctc ttctgcgttt
    11221 aatcccggtg gaacatgctc tgtattaccg tcaaatttgc cctgatcttc cggataacgg
    11281 tacttgtact tgatatgcgt acccagcaga tgaacgatga tcagtttctt cggcgcaggg
    11341 tccttcagca cttcctggaa cggcttcagc acgttggtgt catattcacg cgcactctgc
    11401 gtacgttgct ggttcatgta gtactgcttg tcggtctggc gcgaaaatac cgtcagcatc
    11461 gtattgcggg cggtcatcgt ctgctggttg gtgatccaga aggttttata acccgcctgt
    11521 ttcatcatgt tcatcagcga cggctgcgtc agatacagat ccgggttctt ttcattggca
    11581 aaggtcagcg cctgttgcag gatttcaatg gtgtacggac gagacgtaac cacgttatta
    11641 aacacggtca gattcggatc ggttttatgc agcgcatcca gctccggcgt ggtttcacgc
    11701 ggataaccgt acagactcat gcgtccacgc tgggtcgatt cgccaattac cagcactaag
    11761 gtgcgcggtt cgttacccga ttcatctttg aaattagcca gcggcggcaa ggcattattt
    11821 tcattcagta gctttgttag cgagtttagt tgctgacgat actgataata gccggtcagg
    11881 aattgccacg gtgcggcagg ctccatacgc gatgccaggt tacccaacgt tttctcaaac
    11941 ggcttgttct tgataaacgt attcatggcg atcggatgca gaatcaagcc ataaagcagg
    12001 gcaaaagaga caacataacg ccacggcttt ggaatataga ccgggcgcag gcgtgtccac
    12061 agcagaactg ccaccgccgt ataggccagc gcgataagca caattttcag gctgaaatac
    12121 tggcttaaat actcgctggc ttcgttggtg ttggtttcga acatcacaaa cagaacgctc
    12181 tgcgagaact cctgaccgta gatgacgtaa tagcacagcg ccgccagaga ggccgcccat
    12241 agcaccacgc cgatcactgc ggcaataatt ttaatccgct tcggaaagag gaataccggg
    12301 atcaaccaca gcgaactgaa taacagcgag tcgcgaatgc cgttagtgcc actataacca
    12361 ctgatgtaaa taatggcctg tagcagagta gagaaaaacc aaaagtagag cagtgcccaa
    12421 cccagggctt tccagctaaa aaggggttta gcctggactt ctgtggaatg catagtaaga
    12481 acctgtcttg aaaaaaatat cgccgaatgt aacgacaatt ccttaaggat atctgaaggg
    12541 atatccagat tttgaataaa atgcagacag aaatatattg aaaacgaggg tgttagaaca
    12601 gaagtatttc agaaaaccct cgcgcaaaag cacgagggtt tgcaggagag gaagattagc
    12661 cggtattacg catacctgcc gcaatcccgg caatagtgac cattaacgcc tgctcgacgc
    12721 gaggatccgg ttcctggcct tctttttctg cctggcggga gcggtgcagc aactcggcct
    12781 gcaatacgtt cagcgggtcg gtgtaaatat tccgtagctg aatagactct gcaatccacg
    12841 gcagatcggc catcaggtga gaatcgttgg caatcgccag caccactttg atgtcttctt
    12901 cttgcagatt gcgtaactct ttacctaacg gccacagtgc tttgtctacc aggcgttggt
    12961 catagtattc cgccagccac aggtctgctt tggcgaagac catctccagc atgccgagac
    13021 gcgtcgagaa gaatggccaa tcgcggcaca tggcttccag ttcgctctgt ttgccatctt
    13081 cgaccacttt ttgcagcgcc gtacctgcac ccagccaggc ggggagcatc aggcggtttt
    13141 gcgtccaggc gaaaatccac ggaatagcgc gcagtgactc gacgccgccg gtcgggcgac
    13201 gtttcgccgg acgtgaaccc aacggcagtt tacccaattc ttgttccggc gtagcggagc
    13261 ggaagtaagg cacaaaatct ttgttttcac gtacgtagcc acggtagaga tcgcaggaga
    13321 tgactgacag ttcatccata atgcgacgcc agctctcttt cggctcaggc ggtggcagca
    13381 ggttggcttc cagaatcgcc ccagtgtaca gcgacaggct gctgacggtg atttctggca
    13441 gaccatattt aaagcggatc atctcgccct gttcggtcac gcgcaggcca cctttcaggc
    13501 ttcctggcgg ttgtgacagc agcgccgcat gggcaggtgc gccgccgcga ccaatggaac
    13561 cgccgcgacc gtggaacaac gtcagctcaa tacccgcttt ttcgcaggtt ttgattaatg
    13621 catcctgtgc ctgatattgc gcccaggaag ctgccatcac gcccgcatct tttgctgagt
    13681 cggaatagcc aatcatcacc atctgtttgc cctgaatcag gccgcgatac cagtcgatat
    13741 tcagcagctg ggtcatgaca tcgttggcgt tgttcaggtc atcgagggtt tcaaacagcg
    13801 gagcaaccgg catcgcaaac ccgatacccg cttctttcag cagcaggtgg acagccagca
    13861 cgtcggacgg cgttttcgcc atcgagatca cgtaggcggc aatggagcct tgcggcgctt
    13921 cggcaatcac ctggcaggta tcgagcactt cgcgcgtttc ggcgcttggt tgccagttgc
    13981 gcggtagaag cggacgtttg gagttcagtt cgcggatcag gaacgcctgt ttgtcggctt
    14041 ctgaccagct ctcgtagtcg ccgataccga ggtagcgggt cagctcgccc agcgcttcgg
    14101 tatgacgcgt gctctcctga cggatatcaa tacgcaccag cggtacgccg aaacatttca
    14161 cgcggcgcag ggtgtcgagc agatcgccat tggcgataat acccatgcca cacgcctgaa
    14221 gtgactggta gcaagcgtag agcggttccc acagttcttc gttttgtgtc agcagacctt
    14281 ctggttttgg cagttcttcg cctttcaggc gcgcttccag ccatgcctgt gtcgccatca
    14341 ggcgagaacg caggtttttc atcagatagc gatacggttc tgcggcaccc tcttcgccaa
    14401 ccagcgccag cagttcaggg gtggcttcaa ccatcgacag ttcagaaacc agaacctgaa
    14461 tatctttcag gaacaaatcg gtggctttcc agcggctgag tagcaggacg tggcgggtga
    14521 tatcggcagt gacgttcggg ttgccgtcgc ggtcaccgcc catccacgag gtaaaacgga
    14581 ccggaacaaa ttcgacgggc agtttgtagc cgaggttctc ttccagttgt tcgtttagtt
    14641 cgcgcaggta attcggtacg ccttgccaca ggctgttttc tactacggca aagccccatt
    14701 tggcttcatc taccgggctt gggcgcagtt tacggatttc atcggtatgc catgactggg
    14761 cgatcaactg gcgcaggcgg cgcatcagct ggttgtgttc gtagtcggcg atatctttgt
    14821 tatcgagctg ttttaaacag gcgttcactt ccaccatttt gtggatcagt gtacgacggg
    14881 taatttcggt tgggtgagcc gtgaggacca gttccagcga cagcgattcc actgcttttt
    14941 tgatggtgtc ttcgctcagt tccggctggt ttttcagttt acgcagggtg cgggcgatca
    15001 cttccgggtt gctggcagct tcgcctttcg gcgaaatgct gtggtattgc tcagcggtgt
    15061 tggccaggtt caggaactga ctaaacgcac gcgcaacggg cagcagctcg tcgttcgaca
    15121 aattttgtaa ggtggtgagc aactcctggc ggttagcatc attgccagcg cgtgaagatt
    15181 tcgacaactt acggatagtt tctacgcgtt caagaatgtg ttctcccaac gcatccttga
    15241 tggtttctcc cagcactttg ccgagcatac tgacattact acgcaatgcg gaatattgtt
    15301 cgttcatatt accccagaca ccccatctta tcgtttgata gccctgtatc cttcacgtcg
    15361 cattggcgcg aatatgctcg ggctttgctt ttcgtcgtct tttataaagc cacgtaaaag
    15421 cgatgacgtc aaatgctgcg aaatcgcttc agcaaacgaa taaatagcag gaatttacgc
    15481 cattaaattc acgactcttt aaataagcgt aacttatggg aatgttaaaa aatcgcctcg
    15541 agtaacacca aaggtgtagg tcggataaga tgcgcaagca tcgcatccga cattgtttgg
    15601 cctacgtttt aatgccagca aaaatggtga atcacctggg tgatcagttc gcgggtgggc
    15661 ttgataaacc tcgtctccag atattcatca ggctgatgag cctgattaat tgagccaggc
    15721 cccagaacca gcgtcgggca taacgtttga ataaacggcg cttcggtaca gtaattcacc
    15781 acttcggtct ttgctccgag caatttctca accacttcaa ccagtttatg attcggtggg
    15841 cattcatagc cagggatcgg cggatgcagc tcgtcgaccg tcagacgacc cggccagcgt
    15901 tcgctcactg gagccaatgc atcgttgagc aaaccattaa gttcattgag tgtcatgcct
    15961 ggcagcggac gaatatccat atgcaactca cagcaagcac aaatacggtt agaagcgtcg
    16021 ccaccgtgga tatgcccgag gttgagcgta gggtatggca cggtaaacgc gtcgtagtga
    16081 taacgttctt tcaggttatc gcgcaattgc aaaatatgcc cgatggcgtc gtgcatcagt
    16141 tcgatagcgt taactccgcg tgctggatcg ctggagtggc ccgactggcc ctgaatacgg
    16201 atggcgttag agatatgacc tttatgcgcg cgtaccggtt gcagcgacgt tggctcgcca
    16261 atgatggcgc aatccgggcg cagggcggta gtttcggcaa aataacgcgc tccggccata
    16321 ctggtttctt catcggcagt cgccagaatg tagagcggtt ttgcaagctt cgtgacgtcg
    16381 acatcgcgta gcgcatcaag gataaacgca aaaaagcctt tcatgtcggc ggtgcctaag
    16441 ccgtaaagct tgccgtcatg ctccgtcaac gtaaatggat cgcgcgtcca gcgtccgtca
    16501 tcaaatggca ccgtatcggt atgccccgcc agcaacaaac cgccagcccc ctgtccgcaa
    16561 ctggccagca tattaaattt gttgcgagtt cctggcacag gctgtacttc cacattgaag
    16621 cctaaatcct taaaccagtc cgccagcaga gtgattaaat ctgcattgct ttgatcgagt
    16681 gcctcttccg tggcgcttat tgaaggtgtg gcaatcagag cacggtaaat ctcgataaat
    16741 ggcggtaatt tgtttttcat tgttgacaca cctctggtca tgatagtatc aatattcatg
    16801 cagtatttat gaataaaaat acactaatgt tgagcgtaat aaaacccacc agccgtaagg
    16861 tgaatgtttt acgtttaacc tggcaaccag acataagaag gtgaatagcc ccgatgttga
    16921 atacgctgat tgtgggtgcc agcggctacg ctggcgcaga gctagtgacc tatgtaaatc
    16981 gccatccgca tatgaacata accgctttga ctgtttcagc gcaaagcaat gatgcgggaa
    17041 agttaatctc cgatttgcat ccgcagctaa aaggcatcgt tgatctgccg ttgcagccga
    17101 tgtcggatat cagcgagttt agcccagggg tggacgtggt gtttctcgcc accgcccatg
    17161 aagtcagcca cgatttagcg ccgcaatttc ttgaagcagg ctgtgtggtg ttcgaccttt
    17221 ccggcgcgtt tcgtgttaac gatgtcgcct tctatgaaaa atattacggc tttacccatc
    17281 aatacccgga actgctggaa caggcagcct acggtctggc ggagtggtgc ggtaataaat
    17341 taaaagaagc gaacttgatt gccgtgccag gctgttatcc gacggcagca cagctggcgc
    17401 tgaaaccgtt gattgatgcc gatcttcttg acctcaatca gtggccggtg atcaacgcca
    17461 ccagcggcgt gagcggtgca ggacgtaagg cagcgatttc aaatagtttt tgtgaagtta
    17521 gcctgcaacc gtatggcgtc tttacccatc gccatcaacc agagatcgcc acacacctcg
    17581 gtgctgacgt tatcttcacc ccgcatctgg gcaatttccc gcgcggcatt ctcgaaacca
    17641 ttacctgccg cctgaaaccg ggtgtgagcc aggtgcaagt ggcgcaagcg ttacagcagg
    17701 cgtatgccca taaaccactg gtgcggctgt atgacaaagg cgttccggcg ctgaaaaatg
    17761 tcgttggact gccattctgc gatatcggtt ttgccgttca gggcgagcat ctgattattg
    17821 tggcgaccga agacaactta ctgaaaggcg cggcggcaca agcggtgcag tgcgccaata
    17881 ttcgtttcgg ctatgcggaa acgcagtctc ttatttaagg gtgcaatgat gaatccatta
    17941 attatcaaac tgggtggcgt actgctggat agtgaagagg cgctggaacg tctgtttagc
    18001 gcactggtga attatcgcga gtcacatcag cgtcagctgg tgattgtgca cggcggcggt
    18061 tgcgtggtgg atgagctgat gaaagggctg aatctgccgg tgaaaaagaa aaatggcctg
    18121 cgggtgacgc ctgcggatca gatagacatt atcaccggag cactggcggg aacggcaaat
    18181 aaaaccctgc tggcatgggc gaagaagcat cagattgcgg ccgtaggttt gtttctcggt
    18241 gacggcgaca gcgtcaaagt gacccagctt gatgaagagt taggccatgt tggactggcg
    18301 cagccaggtt cgcctaagct tatcaacagc ttgctggaga acggttatct gccggtggtc
    18361 agctccattg gcgtaacaaa cgaagggcaa ctgatgaacg tcaatgccga ccaggcggcg
    18421 acggcgctgg cggcaacgct gggcgcggat ctgattttgc tctctgacgt cagcggcatt
    18481 ctcgacggca aagggcaacg tattgccgaa atgaccgccg cgaaagcaga acaactgatt
    18541 gagcagggca ttattactga cggcatgatt gtgaaagtga acgcggcgct ggatgctgcg
    18601 cgcacgttgg gccgtccggt agatatcgcc tcctggcgtc atgcggaaca gcttccggca
    18661 ctgtttaacg gtatgccgat gggtacgcgg attttagctt aagttttgtt ggccggaggc
    18721 gcagctttcc ggcattgaat ttcaaaataa ggaaacagag ttatggcact ttggggcggg
    18781 cgttttaccc aggcagcaga tcaacggttc aaacaattca acgactcact gcgctttgat
    18841 taccatctgg cggagcagga tattgttggc tctgtggcct ggtccaaagc cctggtaacg
    18901 gtcggcgtgt taaccgcaga agagcaggcg caactggaag aggcgctgaa cgtgctgctg
    18961 gaagatgttc gcgccaggcc acaacaaatc cttgaaagcg acgccgaaga tatccatagc
    19021 tgggtggaag gcaaactgat cgacaaagtg ggtcagttag gcaaaaaact gcataccggg
    19081 cgtagccgta atgatcaggt agcaactgac ctgaaactgt ggtgcaaaga taccgttagc
    19141 gagttgctga cggctaaccg gcagttacaa tccgctctgg tcgagaccgc gcagaacaat
    19201 caggacgcgg tgatgccggg ttacactcac ctgcaacgcg cccagccggt gacgttcgct
    19261 cactggtgcc tggcctacgt tgagatgctg gcgcgtgatg aaagccgttt gcaggacgcg
    19321 cttaagcgtc tggatgtcag cccgctaggt tgtggcgcac tggcaggaac ggcctatgaa
    19381 atcgaccgtg aacagttagc aggttggctg ggctttgctt cggcgacccg taacagtctc
    19441 gacagcgttt ctgaccgtga ccatgtgttg gaactgcttt ctgctgccgc tatcggcatg
    19501 gtgcatctgt cgcgttttgc tgaagatctg attttcttta acaccggcga agcggggttt
    19561 gtggagcttt ctgaccgtgt gacttccggt tcatcattaa tgccgcagaa gaaaaacccg
    19621 gatgcgctgg agctgattcg cggtaaatgc ggtcgggtgc agggggcgtt aaccggcatg
    19681 atgatgacgc tgaaaggttt gccgctggct tacaacaaag atatgcagga agacaaagaa
    19741 ggtctgttcg acgcgctcga tacctggctg gactgcctgc atatggcggc gctggtgctg
    19801 gacggcattc aggtgaaacg tccgcgttgc caggaagcgg cgcagcaggg ttacgccaac
    19861 gctactgaac tggcggatta tctggtggcg aaaggcgtac cgttccgcga ggcgcaccat
    19921 attgtgggtg aagctgtggt ggaagccatt cgtcagggca aagcgttgga agatctgccg
    19981 ctcagtgagt tgcagaaatt cagccaggtg attggcgaag atgtctatcc gattctgtcg
    20041 ctgcaatcgt gcctcgacaa gcgtgcagca aaaggcggtg tctcaccgca acaggtggcg
    20101 caggcgattg cttttgcgca ggcgcggttg gggtaatttg tttatattgc tcccctgaaa
    20161 atggggagct taccacgcct acaaagtgtg acacttcgta ggcggataag gcgtttacgc
    20221 cgcatcaggc agaaatttgt cagcattctg gtgaccttaa atttaacgcc caaccagaac
    20281 cattatttgt cacattcctt tttattcttg cgtcttcttt accttcactc tccccataaa
    20341 aattatattt tcaatacatc agccaataca tactttaatt aattgaatat aattcatttc
    20401 tattgctgtg ttcattttta atgattaatg gagtttgtta tgtgattatt taacgtgttg
    20461 ttttaacgat gatgg
//
LOCUS       DAZFJL010000085        20211 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFJL010000085 DAZFJL010000000
VERSION     DAZFJL010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.085"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(83..1747)
                     /gene="glnS"
                     /locus_tag="ACHJ8C_003013"
     CDS             complement(83..1747)
                     /gene="glnS"
                     /locus_tag="ACHJ8C_003013"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO5552834.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1967..2728)
                     /locus_tag="ACHJ8C_003014"
     CDS             complement(1967..2728)
                     /locus_tag="ACHJ8C_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5552835.1"
                     /translation="MKGYNRLEQIMDYLKSHNLVTVDELVAVTNASPATIRRDLIKLD
                     EQGVISRTHGGVTLNRFIPTQPTTHEKMQRSLAEKHAIASAAASMVKAGDSVVLDAGT
                     TMIELARQITHLPLRVITSDLHIALFLAEFKQIEVTIIGGRIDDSSQSCIGEHGRKLL
                     QNTWPDVAFLSCNSWDLEKGITAPTEEKAALKRDLIAHASRKILLADSSKYGSWSLFN
                     IAHLNELTEIITDSQLDGQTRRTLASLSTRLIIAD"
     gene            complement(2730..3716)
                     /locus_tag="ACHJ8C_003015"
     CDS             complement(2730..3716)
                     /locus_tag="ACHJ8C_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-threonate 4-phosphate dehydrogenase"
                     /protein_id="HFO5552836.1"
                     /translation="MNNIIAVTMGDPAGIGPEIIIKSLSEAGLAGAPVVVVGCVRTLR
                     RIMAMNITPQAELRVIQKVCDAHFAPGVINVMDEPLANPDALTPGVVQAAAGDLAYRC
                     IKRATALALSGEVKAIATAPLNKEALHLGGHNYPGHTELLAHLTGSKEYAMVLYTDKL
                     KVIHISTHISLRKFLDTLNGERVKTVIRVANHFLKRVGIERPRIAVAGVNPHAGEHGL
                     FGTEEIEIIAPAIEAMQAEDIDVTGPCPPDTVFMQCHEGLFDMVVAMYHDQGHIPLKL
                     LGFYDGVNITAGLPFIRTSADHGTAFDIAWTGKAKSESMAVSIQLAMQISRE"
     gene            complement(3709..4944)
                     /locus_tag="ACHJ8C_003016"
     CDS             complement(3709..4944)
                     /locus_tag="ACHJ8C_003016"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HFO5552837.1"
                     /translation="MEKGITKPSILVVADDFTGANDAGVSLAQVGHTVDVAFEMPYRG
                     DASVWVINSDSRVMDPKQAAMKMTSLMSHLLLANNPPLMIKKIDSTLRGNIGAEIEAL
                     MQACGITGAVVAPAFPQAGRTTVDGECRVNGVRLTETEFASDPKTPVMSARIADIIRL
                     QTAIPCQPVTVSELSHLSREQPWIGVMDAQTDSDLDCIAAAVMQAKQPLLLVGSAGIC
                     DAVARRSTIMPPPTVLAIIGSMSEIAQRQIATLHSHPRITQIYVDVEHILAGNASDYD
                     ARIVQALQKGDHCIVHTCNDSVARHQIDTLCQRWQMSRAALGEKICRFLGELTRQVLL
                     RTMPDALYLSGGDVAMATASALGATGFRITGKVAQCVPYGHFLGGVWSRSVMTKAGGF
                     GDETTLHQVLNFIEEKCSE"
     gene            5114..6271
                     /locus_tag="ACHJ8C_003017"
     CDS             5114..6271
                     /locus_tag="ACHJ8C_003017"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFO5552838.1"
                     /translation="MVIINSTILSGAGAIPSLTSLLPDIRKMLLVTDRNIAQLDGVQQ
                     IRALLEKHCPQVNVIDNVPAEPTHHDVRQLMDAPGDASFDVVVGIGGGSVLDVAKLLS
                     VLCHPQSPGLDALLAGEKPTQRVQSWLIPTTAGTGSEATPNAILAIPEQSTKVGIISQ
                     VLLPDYVALFPELTTSMPAHIAASTGIDALCHLLECFTATVANPVSDNAALTGLSKLF
                     RHIQPAVNDPQDLRAKLEMLWASYYGGVAITHAGTHLVHALSYPLGGKYHLPHGVANA
                     ILLAPCMAFVRPWAVEKFARVWDCIPDAETALSAEEKSHALVTWLQALVNQLKLPNNL
                     AALGVPPEDIASLSEAALNVKRLMNNVPCQLDLQDVQAIYQTLFPQHQFKE"
     gene            6275..7162
                     /locus_tag="ACHJ8C_003018"
     CDS             6275..7162
                     /locus_tag="ACHJ8C_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HFO5552839.1"
                     /translation="MPKTIEGVLTAIVTPFTATGSLNIPALKVQVNRQLEAGNAIFCG
                     GTNGEFFVLNEQEKLSVTQTCVDEVAGRAPVVAHIGEISTRETIRLGQQIEKLGVDAV
                     SVIAPYFVPLKQEELIAHYSAIADALSIPMFLYNIPARTGNTIQPETARALASHPNII
                     GIKDSAGSDESLKGFLDAVRDIDGFNVLNGPDSLIHKGFVEGCSACISGLANIAPREI
                     NAIWSRFHAGDIEGSYEAQESVTGLRTDLYKVAFSPAAVKKALQLMGHDVGDSRYAVN
                     FTPEQISEIQQIIRHYNIN"
     gene            7222..7980
                     /locus_tag="ACHJ8C_003019"
     CDS             7222..7980
                     /locus_tag="ACHJ8C_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO5552840.1"
                     /translation="MFLWFMQPDIWLTLITLTFLEIILGVDNIIFLSLVVAKLPLSQQ
                     NMARKLGLSCAMIMRILLLISIAWLSHITQPLFTVLTLEISFRTLILLGGGLFLIYKA
                     IQEIREELSPAQEDEHGSGSRQLSLCGAIVQIMLLDIVFSLDSVITAVGLSQHIFIMI
                     AAVMIAVGVMMFAAKTIGDFVNATPSIKILALTFLLFVGVLLVADSLNIHIAKEYLYF
                     AIFFSLSVETLNIIRERKNKKVSTLIETHESHSH"
     gene            8016..9185
                     /locus_tag="ACHJ8C_003020"
     CDS             8016..9185
                     /locus_tag="ACHJ8C_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HFO5552841.1"
                     /translation="MSVALIRDGVIRPSENDSHVLTAMLPSSCPQNHAANILPLPDGA
                     LMCVWFAGTQEGIADISVWGSRLPAGGMQWSDAVKLSHDDTRSEQNPVLFLAPDNVLW
                     LLWTAQISGNQDTAIVRYRKSDDLGQTWGEIATLLDKPGTFIRQPITVLDNGNWLLPV
                     FYCRTQPGEKWVGNDDISAVKISADGGHSWRDVEVPQSLGCVHMNITMLHDGTLVALF
                     RSRWADNIYISHSVDNGESWSVPQATELPNNNSSIQVTTLASGELALVYNAMSAAGAV
                     ERRASLYDEIVDGDDSRKEPTAVGRSAFWGAPRAPMTVAISADGGKSWPWRRNLDEGD
                     GYCMTNNSLEKLNREFSYPSIKQSPDGTLHIAYTWWRQAIKYVRISPEWVKGQAS"
     gene            9182..9652
                     /locus_tag="ACHJ8C_003021"
     CDS             9182..9652
                     /locus_tag="ACHJ8C_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO5552842.1"
                     /translation="MIIGNLNHLSLAGLPAWVRNILLRPECSLSALSTREDGRWQPEG
                     CRWFCTLGTSDTQPAELRHTEYHHLWADIQVVITGCEGINAGTRPIARENDEERKPDL
                     FIAPSPENSVAITLHAGDFAVFMPEEPHQALCAIGTATSIRKAVFKVPRDMLEA"
     gene            9666..10946
                     /locus_tag="ACHJ8C_003022"
     CDS             9666..10946
                     /locus_tag="ACHJ8C_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO5552843.1"
                     /translation="MTMEMILALGILVLMIVLIMSDKMPFGAPPLLACLLLVVSGLST
                     VQQAFAGFVNPSVVMIAGFMVVMAALQKTRLISNVKSAMVSLVNKGSYRSYGLLLVIV
                     MLGASLAGTGATGYYVLILSLVSTIPYSKKLPTSKLMMPLGFATNHPLLPINLALLFG
                     VTATVLETAGFHQEISMGRFALVNLIMSAAFLAWSLIAYRFLPDHPIADASEDALAAR
                     EETFNALPAWKEYCTIAAFAVSVIGMMLMNILGNIAFVIPGLAGAFVLMIDVLDFKEV
                     RDHMGAPVILMMAGVIGVADALAGTGFTAMVGDAVAGVLGSGVSPFVFIVAFALLTST
                     CATFTGSNMGSVYIFAPIAIAACTSLGLNPTAAAIAVVISGWNGGYMPIDGMPAMILG
                     MGKYKLPEFWVFSVPMYLIRILALCAGAVFIFPM"
     gene            complement(11035..12981)
                     /gene="nagE"
                     /locus_tag="ACHJ8C_003023"
     CDS             complement(11035..12981)
                     /gene="nagE"
                     /locus_tag="ACHJ8C_003023"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO5552844.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            13314..14114
                     /gene="nagB"
                     /locus_tag="ACHJ8C_003024"
     CDS             13314..14114
                     /gene="nagB"
                     /locus_tag="ACHJ8C_003024"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO5552845.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            14174..15322
                     /gene="nagA"
                     /locus_tag="ACHJ8C_003025"
     CDS             14174..15322
                     /gene="nagA"
                     /locus_tag="ACHJ8C_003025"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO5552846.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLAEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            15331..16551
                     /gene="nagC"
                     /locus_tag="ACHJ8C_003026"
                     /gene_synonym="nagR"
     CDS             15331..16551
                     /gene="nagC"
                     /locus_tag="ACHJ8C_003026"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO5552847.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            16599..17351
                     /gene="nagD"
                     /locus_tag="ACHJ8C_003027"
     CDS             16599..17351
                     /gene="nagD"
                     /locus_tag="ACHJ8C_003027"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO5552848.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            17608..19272
                     /gene="asnB"
                     /locus_tag="ACHJ8C_003028"
     CDS             17608..19272
                     /gene="asnB"
                     /locus_tag="ACHJ8C_003028"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO5552849.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPFNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            19653..19729
                     /locus_tag="ACHJ8C_003029"
     tRNA            19653..19729
                     /locus_tag="ACHJ8C_003029"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19687..19689,aa:Met,seq:cat)
     gene            19739..19823
                     /locus_tag="ACHJ8C_003030"
     tRNA            19739..19823
                     /locus_tag="ACHJ8C_003030"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19773..19775,aa:Leu,seq:tag)
     gene            19847..19921
                     /locus_tag="ACHJ8C_003031"
     tRNA            19847..19921
                     /locus_tag="ACHJ8C_003031"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19879..19881,aa:Gln,seq:ttg)
     gene            19956..20030
                     /locus_tag="ACHJ8C_003032"
     tRNA            19956..20030
                     /locus_tag="ACHJ8C_003032"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19988..19990,aa:Gln,seq:ttg)
     gene            20046..20122
                     /locus_tag="ACHJ8C_003033"
     tRNA            20046..20122
                     /locus_tag="ACHJ8C_003033"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20080..20082,aa:Met,seq:cat)
BASE COUNT         4838 a         5272 c         5170 g         4931 t
ORIGIN      
        1 aacgccgcag agcgcggcgt ttgggataat ttgcatattt cttgccggat gcggcgtaaa
       61 cgctctatcc gacaacgtaa acttactcgc ctactttcgc ccaggtatca cgcagcccaa
      121 cggtgcggtt aaataccggt ttttccgccg tagaatggcg gctgtcgagg cagaagtaac
      181 cttcacgctc aaactggaat gctttacccg caaccgcatc tttcagcgac ggttcagcaa
      241 agccctgttt gatcaccagc gattccgggt taatcaccga caggaaatca tccgcagcac
      301 cagggttcgg cacgctgaac agacggtcat acaaacggat ttcaaccggc agcgcatgtg
      361 ccgcgctcac ccagtgaata acacctttga ctttacgacc atctgccgga tctttgctta
      421 aggtgtcggc gtcataagta cagaagatgg tggtgatatt gccttcggca tctttctcca
      481 cgcgttctgc cttaatgacg taagcattac gcagacgcac ttctttaccc agcaccagac
      541 gtttgtactg cttgttagct tcttcgcgga aatcggcgcg atcgatccaa atctcaccgc
      601 taaatggcac ctgacggctg cccatttccg gtttattcgg atggttcggc atggtaacca
      661 tttcgccttc gccctggtag ttttcgataa ccagtttcac cggatcgata accgccattg
      721 cgcgcggcgc attttcgttg agatcttcac ggatgcagga ttccagtgac gccatctcaa
      781 tggtgttgtc ctgcttggtc acaccaatgc gtttgcagaa ctcacgaata gaagccgcag
      841 tgtaaccacg acgacgcaga ccagaaatgg tcggcatacg cgggtcatcc cagccttcaa
      901 cgtgcttatc ggtcaccagc aggttcagct tacgcttgga catcacagtg tattccagat
      961 tcaggcgcga gaattcgtac tggcgcgggt gaacaggaat ggtgatgttg tccagtaccc
     1021 agtcgtacag acggcggttg tcctggaact caagcgtaca cagagagtgc gtaatacctt
     1081 ccagcgcatc gctgatgcag tgggtgaagt cgtacatcgg gtagatgcac cacttgttgc
     1141 cagtctggtg gtgttcagca aacttaatgc ggtacagcac cggatcgcgc atcacgataa
     1201 acggcgaagc catgtcgatc ttcgcacgca ggcaggcttt accttcttca aaaccgccgg
     1261 tacgcatttt ctcgaacagc gccaggttct cttcaacgct gcgatcgcgg tacgggctgt
     1321 ttttacccgg ctgcgtcaag gtgccgcggt attcgcggat ctgttccggc gtcagttcat
     1381 caacgtacgc caggccttta ttgatcagtt cgatcgcata ggcgtggagc tgatcaaaat
     1441 aatcggagga gtaacggacg ttaccagacc agtggaaacc taaccactct acgtcgtttt
     1501 tgatcgaatc gacatactca atatcttctt ttaccgggtt agtgtcgtcg aaacgcagat
     1561 tgcactggcc tttatagtcc tgggcgatcc cgaagttcag gcagatagat ttcgcatggc
     1621 caatatgcag atagccattc ggctccggcg ggaaacgggt gtgtactgtg gtgtgcttac
     1681 cactggccag atcttcatcg atgatctgac gaataaagtt agtcgggcgg gcttctgcct
     1741 cactcatcgt ggattcctca aagcgtaaac aacgtataac ggcgtatgat cttataagcg
     1801 ggacaggctg acaactgtta gttagagaaa aaatggtttt gcgtaagata ttggggcgtt
     1861 ttctgcgcta ctgccagcag atcctcgcgc tggaaacgta aggccaaacg tcgccagcct
     1921 gacgcattta ttaaattgcc ggatgagctg gccgcagagt catagattag tcggctatga
     1981 ttaaacgcgt cgacaaggac gcaagcgttc tacgcgtctg cccatcaagc tgactatccg
     2041 taataatctc tgtcagttca ttaagatgcg cgatattaaa cagcgaccat gaaccgtatt
     2101 ttgagctgtc agccaggaga attttgcgcg aagcatgcgc gataagatcg cgttttagtg
     2161 cggctttctc ttccgtgggt gctgtaatac ccttttcaag atcccagctg ttacagctta
     2221 aaaatgccac gtccggccag gtattttgca gcaatttacg accatgttcg ccaatgcagg
     2281 actggctgga gtcgtcgata cgtccgccga taatcgtcac ttcaatttgc ttgaattcag
     2341 ccaaaaaaag cgcgatatgc aggtcgctgg tgatcacgcg aagcggtaaa tgtgtaatct
     2401 gccgcgcaag ttcaatcatt gtcgtaccgg catcaaggac gacggaatcc cccgctttga
     2461 ccattgacgc cgctgcgctg gcgatggcat gtttttccgc caggctgcgc tgcattttct
     2521 catgggtagt cggttgggta ggaataaacc ggttgagggt cacaccgccg tgtgtacggc
     2581 tgataacacc ctgctcatcg agttttatca ggtctcgacg aatcgtagcc ggcgacgcat
     2641 tggttaccgc caccagctca tcaacggtga ccagattatg gcttttcagg taatccatga
     2701 tctgctctaa ccggttatat cccttcattt tattctcgcg aaatctgcat tgctaactgg
     2761 atggaaactg ccatgctctc agacttggct tttcctgtcc aggcaatatc aaacgcggtg
     2821 ccgtggtcgg cagaagtgcg aataaatggc aacccggcgg tgatattcac cccatcataa
     2881 aatccaagca gttttaaagg gatatgcccc tgatcgtggt acatagccac taccatatcg
     2941 aatagccctt catggcattg cataaacacg gtatctggcg ggcacgggcc agtaacatca
     3001 atatcttcgg cctgcattgc ttcaatcgca ggcgcgataa tctcgatttc ctcagtgccg
     3061 aataaaccat gttcccctgc atgtggattt acacccgcca cggcaatacg cggacgctca
     3121 ataccaacac gtttcaggaa gtggttcgca acacgaataa ccgttttgac cctttcacca
     3181 ttcaaggtat ccaggaattt acgcagtgag atgtgagtcg aaatatggat aaccttgagc
     3241 ttatcggtat agagcaccat cgcgtactct ttgctgccgg taagatgggc cagcaattcc
     3301 gtatgccccg gatagttatg cccgccaaga tgcagcgctt ctttattcag cggcgcagtg
     3361 gctatggctt tcacctcgcc tgataaggcc agagccgttg cacgtttgat acagcgatac
     3421 gctaaatcgc ctgccgcagc ctgtaccact ccgggtgtca gcgcatcggg gttagccagc
     3481 ggctcatcca tcacgttaat cacgccggga gcaaaatggg cgtcgcacac cttttgaatg
     3541 acgcgtaact ctgcctgcgg cgtgatattc atcgccataa tacgtcgcag cgtgcgcaca
     3601 cagcccacca ccaccaccgg ggcaccagca agcccggctt cgctcaggga tttaataatg
     3661 atctccgggc caatacccgc cgggtcaccc atcgttaccg caataatatt attcactaca
     3721 cttctcctcg ataaaattca gaacctgatg caacgtggtc tcatcgccaa acccaccggc
     3781 tttcgtcatc accgaacggg accagacacc gcccagaaag tgaccgtatg gaacgcattg
     3841 cgccaccttc cccgtaatac gaaatccggt cgcgcctaac gcactggctg ttgccattgc
     3901 gacatcacca ccagagagat aaagcgcatc gggcattgtg cgaagcagaa cttgccgggt
     3961 aagttcgcca agaaaccggc aaattttttc cccaagcgca gcgcgactca tctgccaacg
     4021 ctggcaaagg gtgtcgatct gatgccttgc tacagaatcg ttacaggtgt ggacaatgca
     4081 gtggtcgcct ttctgcaagg cctgcacaat acgcgcgtca tagtcactgg cattgcctgc
     4141 cagaatgtgt tcaacatcga cgtatatctg tgtgatacgt gggtgtgaat gaagtgtggc
     4201 gatctgccgt tgagcgattt cgctcattga accaataatg gccagcacgg tgggtggcgg
     4261 cataatcgtg gagcgccggg caacggcatc acaaatacca gcggagccga ccagcagcag
     4321 cggttgttta gcttgcatca cagcggcagc gatgcaatcc aggtcgctgt ccgtctgcgc
     4381 atccatcacc ccaatccacg gttgctcacg agacaaatgg ctgagctcag aaactgtaac
     4441 gggctggcat ggaatggccg tctgcaaccg aatgatgtcc gcaattcgtg cactcattac
     4501 cggggttttt ggatcgctgg caaactcggt ttccgttagc cgcacgccgt ttacccgaca
     4561 ctcaccgtcc accgtggtcc ttcccgcctg gggaaatgcg ggggcaacca ctgcgcccgt
     4621 tataccgcat gcctgcatca gggcttcaat ttctgcgccg atattgcctc gtagcgtgga
     4681 atctatcttt ttaatcatga gcggcgggtt attcgccaga aggagatgac tcatcagcga
     4741 ggtcattttc atcgcggcct gcttaggatc catcacccgg ctgtcgctat taatcaccca
     4801 tacgctggca tcaccgcgat aaggcatttc aaaggccaca tcaaccgtat gtcccacctg
     4861 cgccaggctt actcctgcgt cgttggcccc tgtgaaatca tcggcaacga cgagaatgct
     4921 cggcttagtg attccctttt ccatcatcac tccaggttgt tttatgataa caatgattat
     4981 attcaatcat gattgattaa atgtgagcaa gctcaagtta ttaatcactc gaataaatta
     5041 taaaattaga ccatgttacg taggctacgg cattttgaat gattgaattc aatcaccaga
     5101 agagaggtta tttgtggtaa ttatcaatag cacgatactg agcggcgcag gcgctatccc
     5161 ttccctgacg tcgctcttac ccgacatcag aaaaatgctg ctggtcactg accgtaatat
     5221 tgcgcagctc gacggtgtgc agcagattcg cgccttactg gaaaagcact gcccgcaggt
     5281 taacgttatc gataatgtgc ccgcagagcc cacgcatcat gatgtgcgcc agctaatgga
     5341 cgcccctggc gatgcctctt ttgatgtggt ggtcgggatc ggcggtggca gcgtgttgga
     5401 tgtggcgaag ctgctatcgg tgctttgcca tccacaatca ccggggctgg atgcgctgct
     5461 tgcgggtgaa aaaccgactc agcgggtgca atcatggttg attcctacaa ccgccggaac
     5521 cggctcagaa gccacgccga atgcgattct ggcaatccct gagcaaagca cgaaggtggg
     5581 tattatttcc caggtgctgt taccagacta tgtggcgctt ttcccggaac tgaccaccag
     5641 catgcccgcg catattgcgg cgtccacggg cattgatgct ctttgccact tactggagtg
     5701 ttttaccgcg accgtggcaa atccggtcag cgataacgcg gcgctgactg ggttaagtaa
     5761 acttttccgg cacattcaac ccgccgtgaa cgatcctcag gatctgcgcg caaaactgga
     5821 aatgctgtgg gcgtcttact atggcggcgt agcgataacc catgcgggca cgcatctcgt
     5881 tcatgcgctc tcctacccgt taggtggcaa atatcatctg ccgcatggcg tcgcgaatgc
     5941 catcttgctg gcgccgtgca tggcgtttgt tcgcccctgg gcggtcgaga aatttgcccg
     6001 ggtctgggat tgcattcccg atgcggaaac cgccctgagc gcggaagaaa aatctcatgc
     6061 cctggtgacc tggttacagg cattagtcaa tcaactcaag ctacccaaca atctcgcggc
     6121 tctcggcgta ccgccagagg atattgcctc tctgagcgag gcggcactga acgtgaagcg
     6181 ccttatgaac aatgtgccgt gccagcttga tctacaggac gtacaggcca tttaccaaac
     6241 actgtttccg caacatcaat ttaaggaata aatcatgccg aaaaccattg aaggtgtatt
     6301 aaccgctatc gtcaccccgt ttaccgcgac cggcagtctg aatatcccgg cgttaaaagt
     6361 gcaggtgaat cgtcaactgg aagcgggtaa cgcgattttt tgcggcggca ccaacggcga
     6421 attttttgtg ttgaatgaac aggagaagct cagcgttacg caaacctgcg tggatgaagt
     6481 ggctggtcgt gcgccggtcg tggcacacat tggcgagatc tctacgcgcg aaacgattcg
     6541 tctgggtcag cagattgaaa aacttggcgt agatgccgtg tcggtcattg ccccttactt
     6601 cgtaccgctg aaacaggaag agttgattgc gcattacagc gcaattgccg acgcactgag
     6661 cattccgatg tttctctata acatcccggc ccgcactggc aatacgatcc agccagaaac
     6721 cgcgcgtgct ctggcgtcgc accccaatat cattgggatt aaagacagcg ccggaagcga
     6781 cgaaagcctg aagggttttc tcgatgcggt gcgcgacatt gacggcttta atgtgctcaa
     6841 cggcccggat tcgctgatcc ataaagggtt tgtcgaggga tgttcagcct gtatctctgg
     6901 gctcgccaac atcgcgccgc gagaaatcaa tgccatctgg tctcgcttcc atgccggtga
     6961 tattgaaggg tcttatgaag cacaggagag tgtaacgggc ctgcgtaccg atctttataa
     7021 agtagcattc tccccggctg cggtgaaaaa agcgttgcag cttatggggc atgatgttgg
     7081 tgatagccgc tacgcggtta atttcacgcc tgaacagatc agcgagattc aacaaattat
     7141 tcgtcattat aacattaatt aaattaactc tattatccag cccgctataa agtttagata
     7201 aagagaataa aggattaccc catgtttttg tggtttatgc agccagatat ctggctcact
     7261 ttaattaccc tcacctttct ggaaattatt cttggggtgg ataatattat ttttctttca
     7321 ctggtggtgg caaaacttcc attatcacaa caaaatatgg cgcgtaaact gggtttaagc
     7381 tgcgcgatga ttatgcggat attattgctg atatccattg cgtggctttc acatattacc
     7441 cagccgcttt ttaccgtgct gacgctggag atttcgttcc gtaccttgat tctgctcggg
     7501 ggcgggctgt tcctgattta taaagcgata caggagatac gcgaagagct gtcgcccgcg
     7561 caggaagacg aacatggcag cggatcgcgg caactgtcgt tgtgcggtgc cattgtacag
     7621 ataatgctgc tcgatatcgt gtttagcctg gattcggtga tcaccgccgt cggcctttct
     7681 cagcatatat ttatcatgat tgccgccgta atgattgccg tcggtgtcat gatgtttgcg
     7741 gcgaaaacaa ttggtgattt cgttaacgcc acgccgtcaa taaaaatact ggcgttaacc
     7801 tttttattgt ttgtcggtgt attgctggtt gccgatagtc tcaacattca tattgccaaa
     7861 gagtatctct acttcgcgat atttttctca ttaagtgttg agacactcaa tattatccgt
     7921 gaaagaaaaa ataaaaaagt ttcgacattg atcgaaacgc atgaatcgca ctcgcattaa
     7981 taaatctatt accaacttta agatggagtc taactatgtc tgtagccctt attcgtgacg
     8041 gcgtcatccg tccatccgaa aacgattcgc atgtgcttac cgccatgctg ccctcgtcct
     8101 gcccgcaaaa ccacgcggcg aatattctgc ctttgccgga tggcgcgtta atgtgcgtct
     8161 ggtttgcggg aacgcaggaa ggtattgccg atatttctgt ctggggatcg cgattgcccg
     8221 ctggcggaat gcagtggagc gatgcggtca aactttccca cgatgacacg cgttcagagc
     8281 aaaacccggt gctgtttctc gcgccggata acgtgctgtg gctcctatgg accgcacaaa
     8341 tatccggtaa tcaggacacc gccatcgtcc gctaccgtaa gtcagacgac ctcggacaaa
     8401 cctggggcga gattgccacc ctgcttgata aacccggtac gttcattcga cagccaatca
     8461 cggtgcttga taacggcaac tggctgctgc ccgtctttta ttgccgtacc caacccggcg
     8521 aaaaatgggt cggcaatgat gatatcagcg cagtgaaaat ttccgctgac ggtggtcact
     8581 cctggcgcga tgtcgaggtg ccgcaaagtc tcggctgtgt tcatatgaat atcacaatgt
     8641 tgcacgacgg tacgttggtt gctctctttc gcagccgttg ggcagacaac atctatatca
     8701 gccattcagt ggacaacggc gagagttggt cggtgccgca agcaaccgaa ttgcccaaca
     8761 acaattcgtc tattcaggta acgacgcttg ccagcggcga actggcgctg gtgtacaacg
     8821 cgatgagtgc ggcaggcgca gtagaacgtc gcgcgtcgct ttatgacgaa atcgtcgatg
     8881 gcgacgacag ccgcaaagaa ccgacagccg tcggacgctc cgctttctgg ggtgccccac
     8941 gcgcgccgat gacggtagcg atttctgccg atggtgggaa aagctggccg tggcggcgca
     9001 atctcgatga aggcgacggc tactgcatga ccaacaattc cctggaaaag ctaaatcgtg
     9061 agttctccta ccccagcatc aaacaaagcc cggacggtac gctacatatc gcttacacct
     9121 ggtggcgtca ggccatcaag tacgtacgca tctcccccga gtgggttaag gggcaggcat
     9181 catgattatc ggtaatctca accatttgtc gctggcgggg cttcctgcct gggtgagaaa
     9241 tattctgctg cgcccggagt gttcgttatc ggcgttatcc accagagagg acggtcgctg
     9301 gcaacctgaa ggatgtcggt ggttctgcac gctgggaact tcagacacgc aacccgccga
     9361 actccgccat acggagtatc accatctatg ggccgatata caggtggtga ttactggttg
     9421 tgagggcatt aatgcgggaa cgcggcccat cgctcgcgaa aacgatgaag aacgtaagcc
     9481 cgatctgttt atcgccccat cccctgaaaa cagtgtcgcc atcaccctac acgccggtga
     9541 tttcgccgtc tttatgcctg aggaacctca ccaggcgctg tgcgcaattg gcacggctac
     9601 ttcaatacgc aaagcggtct ttaaagtacc gcgcgacatg ctggaagcct aaaaggagag
     9661 aaaaaatgac aatggaaatg atcctggcat taggaatact ggtcctgatg atcgtgctga
     9721 tcatgtcgga caaaatgcct tttggcgcac cgccactgct tgcgtgttta ttgctggttg
     9781 tatcgggact ttcgaccgtt caacaggctt ttgccggatt cgtcaatcca agcgtggtga
     9841 tgattgcagg gtttatggtg gtcatggcgg cgctacaaaa aacccggctt atcagcaacg
     9901 ttaaatctgc gatggtaagc ctggtcaata aaggcagtta ccgcagctat gggctgttgt
     9961 tagtcattgt tatgctgggt gccagtcttg ccggcaccgg agcgaccggt tattacgtgc
    10021 taattctgtc gctggtatcc accattccgt acagtaaaaa gctaccaaca tcgaaattaa
    10081 tgatgcccct gggatttgcc accaaccacc cgcttcttcc tatcaatctc gcgcttctct
    10141 ttggcgttac ggcgaccgta ctcgaaaccg cggggtttca tcaggagatt tcgatggggc
    10201 gctttgcgct ggtgaatctc atcatgtcag cggctttcct cgcatggagt cttattgcct
    10261 accgttttct tccggatcat cccattgcgg atgcttccga ggatgcgctg gcggcacgcg
    10321 aggaaacgtt taacgccctt cctgcctgga aagaatattg cactattgcc gcattcgctg
    10381 tcagcgttat cggcatgatg ctaatgaaca ttctgggaaa tatcgccttc gttattccgg
    10441 gtcttgcggg tgcgtttgtt ctgatgatcg atgtactgga cttcaaagaa gttcgcgatc
    10501 atatgggggc tccggtcatt ttaatgatgg cgggggtgat cggtgtagct gatgcactgg
    10561 cgggcaccgg ctttacggcg atggttggcg atgccgttgc aggtgtgctc ggttctggcg
    10621 tctcaccgtt tgtatttatt gtcgcctttg ctttgcttac cagtacctgc gccaccttca
    10681 cgggttcgaa tatggggtca gtatatattt ttgcccccat cgccattgcc gcctgtacta
    10741 gccttgggct taacccgacc gctgccgcga ttgcggtggt gatttctggc tggaatggcg
    10801 gctatatgcc tattgacgga atgccagcca tgatcctcgg aatggggaaa tacaaacttc
    10861 ccgaattctg ggtgttctct gtaccaatgt atctcatccg cattctggcg ctttgtgcag
    10921 gcgctgtttt tatcttccca atgtaatctc agacaaaaaa agcggcagag aagatccctg
    10981 ccgcttttta ttgttggatg cgacgctgcc gcgtcgcatc agacataaag cagattactt
    11041 tttgatttca tacagcggtg tttgacccgc cacaacatgg ccctgagctt taatgataag
    11101 gccgctgaaa tcgtcgatat tgctgcaaac caccgggcta atcatcgagc gagcgttagc
    11161 gttcaggtaa tccagatcca tttccagaat cggttgccct gcgcttacct gcgcgccctc
    11221 ttccaccaga cgtttaaagc ctttaccttc cagcgctacg gtgtcgatac ccatatggac
    11281 gacgatctcc gcgccttttt cggtttccag acagaatgcg tggttggtgt tgaagatttt
    11341 cacgattgtt cctgcggctg gtgatacgac gattttatct gtcggtttca ccgccacacc
    11401 gtcacccacc gctttgctgg cgaatgcttc gtcaggaacc tgatccagtg ccacaacatc
    11461 cccggtaatc ggcgatacca gctccgcgat agataccgcg tttggtacag cctgcggttt
    11521 tgctacagga gcggcagttg ccggagttgc ttcagctgac gcagcggcta ccggaccacg
    11581 ggcaacgact ttcttcatcg catcgccgat ggattctgct ttcgcgccaa caatcacctg
    11641 aatagtctgt ttgttcagtt tcactacccc agaagcaccc agacgtttac acatcgcatc
    11701 gttgacgcga gctgagtcaa ccacggtaag gcgcagacga gtaatacagg cgtcaatggc
    11761 tttcaggttg tcagtaccgc caaccgcagc aatatagttg gttgccagtt gattgagacc
    11821 ttcttcagta ttgctgttag cctcttcagt aacgatctcg tcttctttat cttcacgacc
    11881 cggcgttttc aggttgaaca tgcggataac caaactgaac accacgaagt agatagcgaa
    11941 gaagacaacc cccatcacca gcagcatcca gacgttctgg ctggcggccg gcaggttata
    12001 catcaacgcg tagtcgatag cccccgcaga gaaagagaag ccggcatgga tacccagcag
    12061 cgttgccaca aacaggctga taccggtcag cagtgcgtgc aggaggtaca gcagcggtgc
    12121 aaggaacatg aacaggaatt ccagcggctc agtcacaccg gtcaggaacg cagtaacagc
    12181 aacagaaagc agcatcccgc caaccatcgg acgacgctct ttcggtgctg cgaagtacat
    12241 cgccagcgct gcacccggca gaccgaacat cataatcggg aagaagccgg acatgaacat
    12301 ccccgcggtg ccgtcaccgg catagaagcg gttgatatca ccgtggaaaa ccgttcccgc
    12361 cgcgttggtg aattcaccaa tctggaacca ggcgatggtg ttcagcacct gatgcagacc
    12421 ggttgggatc agcagacggt tgatgaaacc aaagataccg gaacccagcg cgcccgcaga
    12481 aacgatccac tcgccgcctg catggatagc gtgctgtacc ggtggccaga cgtaaccaaa
    12541 aatggccgcc agcaccagac agaagaagcc ggtggctatc ggcacaaagc gtttgccgcc
    12601 gaagaagctc aggaagtccg gcagtttaat atcggaccaa cggttatagg ctgcgccacc
    12661 aaccagaccg gtaatgatac ccgccagtac acccatgtta atttctgggt tgatggtcac
    12721 catcgctttg gttaacacaa agtaacctac cgcacccgcc agtgccgccg cacctgcgct
    12781 gtctttcgac cagctggatg ccacgccgat ggcgaatatt aatgcgaggt tatcaaaaat
    12841 cgcaccgccc gcctgggcaa taaacgcaac gttaagtaaa tctggctgac cgaatcgcag
    12901 caacagcgcc gccaccggca gcaccgcgat agggagctgt aacgccctac cgagtcgctg
    12961 gaaaaaacct aaaatattca tcttattccc cctacgagaa ccctatttgg ctcgtttaaa
    13021 gccgtatttt tattttgctg caaattgtac tgccgatgtt ctgtaatcag attgttagat
    13081 catctgctac agagtgtgtg aaaatttaat tcgtatcgca aattaaacgc gtgtcttttg
    13141 tgagttttgt caccaaatat cgttatcatc actccctttt actggctaaa ccagaaaact
    13201 tattttatca ttcaaaaaat caggtcggat tgacgcctgt ctgcgcaaat ccaggttacg
    13261 cttaaagatg cctaatccgc caacggctta cattttactt attgaggtga ataatgagac
    13321 tgatccccct gactaccgct gaacaggtcg gcaaatgggc tgctcgccat atcgtcaatc
    13381 gtatcaatgc gttcaaaccg actgccgatc gtccgtttgt actgggcctg ccgactggcg
    13441 gcacgccgat gaccacctat aaagcgttag tcgaaatgca taaagcaggc caggtcagct
    13501 ttaagcacgt tgtcaccttc aacatggacg aatatgtcgg tctgccgaaa gagcatccgg
    13561 aaagctacta cagctttatg caccgtaatt tcttcgatca cgttgatatt ccagcagaaa
    13621 atatcaacct tctcaacggc aacgccccgg atatcgacgc cgaatgccgc cagtatgaag
    13681 aaaaaatccg ttcttacgga aaaattcatc tgtttatggg cggtgtaggt aacgacggtc
    13741 atattgcatt taacgaaccg gcgtcttctc tggcttctcg tactcgtatc aaaaccctga
    13801 ctcatgacac tcgcgtcgcg aactctcgtt tctttgataa cgatgttaat caggtgccaa
    13861 aatatgccct gactgtcggt gttggtacat tgctggatgc cgaagaagtg atgattctgg
    13921 tgctgggtag tcagaaagca ctggcactgc aggccgccgt tgaaggttgc gtgaaccaca
    13981 tgtggaccat cagttgtctg caactgcatc caaaagcgat catggtgtgc gatgaacctt
    14041 ccaccatgga gctgaaagtt aagactttaa gatatttcaa tgaattagaa gcagaaaata
    14101 tcaagggtct gtaattgtta tccctgccct ggctccttgc tcagggcatt attttttaaa
    14161 atcgggggtc agaatgtatg cattaaccca gggccggatc tttaccggcc acgaatttct
    14221 tgatgaccac gcggttgtta tcgctgatgg cctgattaaa agcgtctgtc cggtagcgga
    14281 actgccgcca gagatcgaac aacgttcact gaacggggcc attctctccc ccggttttat
    14341 cgatgtgcag ttaaacggct gcggcggcgt acagtttaac gacaccgctg aagcggtcag
    14401 cgtggaaacg ctggaaatca tgcagaaagc caatgagaaa tcaggctgta ctaactatct
    14461 gccgacgctt atcaccacca gcgatgagct gatgaaacag ggcgtgcgcg ttatgcgcga
    14521 gtacctggca aaacatccga accaggcgtt aggtctgcat ctggaaggtc cgtggctgaa
    14581 tctggtaaaa aaaggcaccc ataatccgaa ttttgtgcgt aagcctgatg ccgcgctggt
    14641 cgatttcctg tgtgagaacg ccgacgtgat taccaaagtg actctggcac cggaaatggt
    14701 tccagcggaa gtcatcagca aactggcaaa tgccgggatt gtggtttctg ctggtcactc
    14761 caacgcgacg ttgaaagaag cgaaagccgg tttccgcgcg gggattacct ttgccaccca
    14821 tctgtacaac gcgatgccgt atattaccgg tcgtgaaccg ggcctggcgg gcgcgatcct
    14881 cgacgaagct gacatttatt gcggtattat cgctgatggc ctgcatgttg attacgccaa
    14941 cattcgtaac gctaaacgtc tgaaaggcga caaattgtgt ctggttaccg atgccaccgc
    15001 gccagcaggt gccaacattg aacagttcat ttttgcgggt aaaacaatat actaccgtaa
    15061 tggactttgt gtggatgaga acggtacgtt aagcggttca tccttaacca tgattgaagg
    15121 cgtgcgtaat ctggccgaac attgtggtat cgcactggat gaagtgctgc gtatggcgac
    15181 gctctatccg gcacgtgcga ttggcgttga gaaacgtctc ggcacgctcg ccgcaggtaa
    15241 agtagccaac ctgactgcat tcacacctga ttttaaaatc accaggacca tcgttaacgg
    15301 taacgaggtc gtaactcaat aagagaaagt atgacaccag gcggacaagc tcagataggt
    15361 aatgttgatc tcgtaaaaca gcttaacagc gcggcggttt atcgcctgat tgaccagtac
    15421 gggccaatct cgcggattca gattgccgag caaagtcagc ttgcccccgc cagcgtaacc
    15481 aaaattacgc gtcagcttat cgaacgcggg ctgatcaaag aagttgatca gcaggcctcc
    15541 accgggggcc gccgcgctat ctccatcgtc accgaaaccc gcaatttcca cgcaatcggc
    15601 gtacggcttg gtcgtcacga cgccaccatc accttgtttg atctcagcag caaagtgctg
    15661 gcagaagaac attacccgct gccggaacgt acccagcaga cgctggaaca tgccctgctg
    15721 aatgccattg ctcagtttat tgatagctac cagcgcaagc tacgcgagct gatcgcgatt
    15781 tcggtgatcc tgccagggct tgtagacccg gacagcggca aaattcatta catgccgcat
    15841 attcaggtag aaaactgggg gctggttgaa gcactggaag agcgttttaa agtgacctgt
    15901 tttgtcggcc acgatatccg cagtctggcg ctggcagagc actacttcgg tgcaagtcag
    15961 gattgcgaag actccattct ggtgcgtgtc catcgcggaa ccggggccgg gattatctct
    16021 aacgggcgaa tttttatcgg gcgcaacggc aacgtcggtg aaattggcca tattcaggtc
    16081 gaaccgctgg gtgaacgctg ccactgcggc aactttggct gcctggaaac tatcgccgcc
    16141 aatgctgcca ttgaacaacg ggtgttgaat ctgttaaagc agggctacca gagccgtgtg
    16201 cctctggacg actgcaccat caaaactatc tgcaaagccg cgaacaaagg cgatagcctg
    16261 gcctcggaag tgattgaata tgtcggtcgt catctgggta aaaccatcgc cattgctatc
    16321 aacttattta atccgcaaaa aattgttatt gctggagaaa tcaccgaagc cgataaagtg
    16381 ctgctccctg ctattgaaag ctgcattaat acccaggcgc tgaaggcgtt tcgtactaat
    16441 ctaccggtgg tacgttctga gttggatcac cgctcggcaa tcggcgcttt tgcgctggta
    16501 aaacgcgcca tgctcaacgg tattttgctc cagcatttgc tggaaaatta atgtgctttt
    16561 atagtggcgc ttattgttgt caatattctg ggtagtccat gaccattaaa aatgtaattt
    16621 gcgatatcga cggcgtgctg atgcacgata acgtcgccgt accgggtgca gcggaatttt
    16681 tgcacgggat tatggataaa ggcctgccgc tggtgttgct gaccaactat ccttcgcaga
    16741 ccgggcaaga tctggcgaac cgctttgcca ctgctggtgt cgatgtaccg gatagcgtgt
    16801 tttatacctc cgcgatggcg accgccgatt tcctgcgtcg ccaggaaggc aagaaagcgt
    16861 atgtggtggg cgaaggcgca ctgattcatg aactgtacaa agccggtttc actattaccg
    16921 atgtgaaccc tgattttgtg attgttggcg aaacgcgttc ctacaactgg gacatgatgc
    16981 ataaagcagc ctatttcgtc gctaacggtg cacgctttat cgccaccaac ccggacaccc
    17041 acgggcgcgg tttttatccc gcctgtggcg cgttgtgtgc agggatcgag aaaatctccg
    17101 ggcgcaaacc gttctatgtt ggaaagccga gtccgtggat catccgcgcg gcattaaaca
    17161 aaatgcaggc gcattcggaa gaaacggtga ttgtcggcga taacttgcgt accgatattc
    17221 tggcgggctt ccaggccggt ctggagacga ttctggtgct ttctggtgtt tcgtcgctcg
    17281 acgatatcga cagtatgccg ttccgcccaa gttggattta cccatcggtc gctgaaatcg
    17341 acgttatctg aatagcaacc acgcctcagg gcgtggtttc ttatttttac ccatcattaa
    17401 aatcacccta ttcaataaaa cccgtcaaaa attatcgatc catcaataaa tatacccaaa
    17461 ggccctcccc tttttcatca atcagcaatc gccattacgt tttttatttt tccaccgcca
    17521 aatcgcttgc gcaccgtgct gctttgcggc atttttttaa acaagcaaac acaacaagca
    17581 acaaatacca ggttaacgga gaaggttatg tgttcaattt ttggcgtatt cgatatcaaa
    17641 acagacgcag ttgagctgcg taagaaagca ctcgagctgt cacgcctgat gcgtcatcgt
    17701 ggcccggact ggtccggcat ttatgccagc gataacgcca ttctcgccca cgaacgtctg
    17761 tcaattgttg acgttaacgc gggggcgcaa cctctctaca accaacaaaa aactcatgtg
    17821 ctggcagtaa acggtgaaat ctacaaccac caggcattgc gcgctgaata tggcgatcgt
    17881 taccagttcc agaccgggtc tgactgtgaa gtgatcctcg cgctgtatca ggaaaaaggg
    17941 ccggaatttc tcgacgactt gcagggcatg tttgcctttg ccctgtacga cagcgaaaaa
    18001 gatgcatatc tgattggtcg cgaccatctg gggatcatcc cactgtatat gggctatgac
    18061 gaacacggtc agctgtatgt ggcctcagaa atgaaagccc tcgtgccagt ttgccgcacg
    18121 attaaagagt ttccggcggg gagctatttg tggagtcagg acggcgaaat ccgttcttat
    18181 tatcatcgcg actggttcga ctacgatgcg gtgaaagata acgtgaccga caaaaacgag
    18241 ctgcgtcagg cactggaaga ttccgttaaa agccatctga tgtctgatgt gccttacggt
    18301 gtgctgcttt ctggtggtct ggattcctca attatttccg ctatcaccaa gaaatacgcc
    18361 gcccgtcgcg tggaagatca ggaacgctct gaagcctggt ggccgcagtt gcactccttt
    18421 gctgtaggtc tgccgggttc accggatctg aaagcagccc aggaagtggc aaaccatctg
    18481 ggcacggtgc atcacgaaat tcacttcact gtacaggaag gtctggatgc catccgcgac
    18541 gtgatttacc acatcgaaac ttatgatgtg accactattc gcgcttcaac accgatgtat
    18601 ttaatgtcgc gtaagatcaa ggcgatgggc attaaaatgg tgctgtccgg tgaaggttct
    18661 gacgaagtgt ttggcggtta tctatacttc cacaaagcgc cgaacgctaa agagttgcat
    18721 gaagagacgg tgcgtaaact gctggccctg catatgtatg actgcgcgcg tgccaacaaa
    18781 gcgatgtcag cctggggcgt ggaagcacgc gttccgttcc tcgacaaaaa attccttgat
    18841 gtggcgatgc gcattaaccc acaggataaa atgtgcggta acggcaaaat ggaaaaacac
    18901 atcctgcgtg aatgttttga agcgtatctg cctgcaagcg tggcctggcg gcaaaaagag
    18961 cagttctccg atggcgtcgg ttacagttgg atcgacaccc tgaaagaagt ggcagcgcag
    19021 caggtttctg atcaacagct ggaaactgcc cgcttccgct tcccgttcaa cacgccgacc
    19081 tcaaaagaag cgtatctgta ccgggagatc tttgaagaat tgttcccgct tccgagcgcc
    19141 gctgagtgcg tgccgggcgg tccttccgtc gcttgttctt ccgctaaagc gattgagtgg
    19201 gatgaagcgt tcaagaaaat ggacgatccg tctggtcgcg cggttggcgt tcaccagtcg
    19261 gcatataaat aagacgtgta atattgcccc ggagaaatcc ggggcaattt cagttactgg
    19321 ggcgcttgtt gattctgata tgcagtaagg aaatgttccg ggtttgccac atcgctgttt
    19381 gacaactcat tactgtaggc cagtatttca tagattaatt tcccaccact attgttgaat
    19441 tccgaatttc atcctgtatg acaaaagaat ggctgtaggg tggggaaaaa acacaaagta
    19501 aacaataatt gacgaatata gcgccatgct gttcgcaacc taaccaaaca gtcactttcg
    19561 agcaattttc cttgaaaaag aggttgacgc tgcaaggctc tatacgcata atgcgccccg
    19621 caacgccgat aaggtatcgc gaaaaaaaag atggctacgt agctcagttg gttagagcac
    19681 atcactcata atgatggggt cacaggttcg aatcccgtcg tagccaccat ctttttttgc
    19741 gggagtggcg aaattggtag acgcaccaga tttaggttct ggcgccgcaa ggtgtgcgag
    19801 ttcaagtctc gcctcccgca ccattcacca gaaagcgttg tacggatggg gtatcgccaa
    19861 gcggtaaggc accggttttt gataccggca ttccctggtt cgaatccagg taccccagcc
    19921 atattcttcg aataagcggt tcaccgcccg gttattgggg tatcgccaag cggtaaggca
    19981 ccggtttttg atgccggcat tccctggttc gaatccaggt accccagcca tcgaagaaac
    20041 aatctggcta cgtagctcag ttggttagag cacatcactc ataatgatgg ggtcacaggt
    20101 tcgaatcccg tcgtagccac caaattctga atgtgtcgaa tatgttcggc aaattcaaaa
    20161 accaatttgt tggggtatcg ccaagcggta aggcaccgga ttctgattcc g
//
LOCUS       DAZFJL010000086        19742 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFJL010000086 DAZFJL010000000
VERSION     DAZFJL010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.086"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..1535
                     /locus_tag="ACHJ8C_003034"
     CDS             <1..1535
                     /locus_tag="ACHJ8C_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFO5552850.1"
                     /translation="YYPGGNLLNASDKLFYLPFAFIIIVLLVVYLMTTRVFRRQFSEM
                     TDLVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIERANKLLSLISYD
                     QESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYA
                     QCCDIVTFNADLYLLLCRENVQTFTRKIATVNDFDSSFGYRNLRIHKSAICEPLQGEN
                     AWSYAEKLKLAISSIRNHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQ
                     PIVDINTRTILGAEALCRWVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFR
                     HFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERI
                     NEHFYLNIEQLRKQGVRVSIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKI
                     VRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLV
                     KE"
     gene            1543..2871
                     /locus_tag="ACHJ8C_003035"
     CDS             1543..2871
                     /locus_tag="ACHJ8C_003035"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO5552851.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKSVTGDEYNAKNAIFTVSEAMEHFSYLPTGLYVF
                     AYKKDVYFQVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(2918..4243)
                     /gene="rimO"
                     /locus_tag="ACHJ8C_003036"
     CDS             complement(2918..4243)
                     /gene="rimO"
                     /locus_tag="ACHJ8C_003036"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO5552852.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWRKICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            4456..4839
                     /gene="bssR"
                     /locus_tag="ACHJ8C_003037"
     CDS             4456..4839
                     /gene="bssR"
                     /locus_tag="ACHJ8C_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO5552853.1"
                     /translation="MFVDRQRIDMLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            4950..6065
                     /gene="yliI"
                     /locus_tag="ACHJ8C_003038"
     CDS             4950..6065
                     /gene="yliI"
                     /locus_tag="ACHJ8C_003038"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO5552854.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(6062..6688)
                     /gene="gstB"
                     /locus_tag="ACHJ8C_003039"
     CDS             complement(6062..6688)
                     /gene="gstB"
                     /locus_tag="ACHJ8C_003039"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO5552855.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            6934..8136
                     /gene="dacC"
                     /locus_tag="ACHJ8C_003040"
     CDS             6934..8136
                     /gene="dacC"
                     /locus_tag="ACHJ8C_003040"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO5552856.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(8183..8941)
                     /gene="deoR"
                     /locus_tag="ACHJ8C_003041"
     CDS             complement(8183..8941)
                     /gene="deoR"
                     /locus_tag="ACHJ8C_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO5552857.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(8999..9595)
                     /gene="ybjG"
                     /locus_tag="ACHJ8C_003042"
     CDS             complement(8999..9595)
                     /gene="ybjG"
                     /locus_tag="ACHJ8C_003042"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO5552858.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            9880..11112
                     /gene="mdfA"
                     /locus_tag="ACHJ8C_003043"
     CDS             9880..11112
                     /gene="mdfA"
                     /locus_tag="ACHJ8C_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO5552859.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            complement(11153..11437)
                     /gene="ybjH"
                     /locus_tag="ACHJ8C_003044"
     CDS             complement(11153..11437)
                     /gene="ybjH"
                     /locus_tag="ACHJ8C_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO5552860.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(11522..12337)
                     /gene="ybjI"
                     /locus_tag="ACHJ8C_003045"
     CDS             complement(11522..12337)
                     /gene="ybjI"
                     /locus_tag="ACHJ8C_003045"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO5552861.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFNQ"
     gene            complement(12337..13545)
                     /locus_tag="ACHJ8C_003046"
     CDS             complement(12337..13545)
                     /locus_tag="ACHJ8C_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5552862.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            13629..14165
                     /gene="rcdA"
                     /locus_tag="ACHJ8C_003047"
     CDS             13629..14165
                     /gene="rcdA"
                     /locus_tag="ACHJ8C_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO5552863.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSRFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERIAG"
     gene            complement(14341..16026)
                     /locus_tag="ACHJ8C_003048"
     CDS             complement(14341..16026)
                     /locus_tag="ACHJ8C_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO5552864.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            16296..16673
                     /locus_tag="ACHJ8C_003049"
     CDS             16296..16673
                     /locus_tag="ACHJ8C_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO5552865.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSHRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(16703..16960)
                     /gene="grxA"
                     /locus_tag="ACHJ8C_003050"
     CDS             complement(16703..16960)
                     /gene="grxA"
                     /locus_tag="ACHJ8C_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO5552866.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            17120..17407
                     /locus_tag="ACHJ8C_003051"
     CDS             17120..17407
                     /locus_tag="ACHJ8C_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO5552867.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNHPPQYSRSEREKDNDANH"
     gene            17391..18113
                     /gene="nfsA"
                     /locus_tag="ACHJ8C_003052"
     CDS             17391..18113
                     /gene="nfsA"
                     /locus_tag="ACHJ8C_003052"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO5552868.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            18174..19076
                     /gene="rimK"
                     /locus_tag="ACHJ8C_003053"
     CDS             18174..19076
                     /gene="rimK"
                     /locus_tag="ACHJ8C_003053"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO5552869.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            19164..19640
                     /locus_tag="ACHJ8C_003054"
     CDS             19164..19640
                     /locus_tag="ACHJ8C_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO5552870.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
BASE COUNT         5023 a         4710 c         5011 g         4998 t
ORIGIN      
        1 tttattatcc tggtggtaat ttattgaacg catcagataa acttttttat ctgccgtttg
       61 ctttcattat tatcgtattg ctggtggttt atttaatgac cactcgagtg ttccgtcggc
      121 aattttctga aatgacagat ttggtcaata cgctggcgtt tttgcccgac tctacggatc
      181 aaatcgaggc cctgaagatt cgtgaaggcg atgcgaaaga gattatcagc atcaaaaatt
      241 cgatcgcgga aatgaaagat gctgaaattg aacgggcaaa taaattgctc tcgctgatct
      301 cttacgatca ggaaagcggt tttattaaaa atatggcgat tattgagtcc aacaataatc
      361 agtatctggc tgtggggatc atcaaactgt gtggtctgga agctgtggaa gcggtgtttg
      421 gtgttgatga acgcaataaa atcgtcagga aattgtgtca gcgaattgcc gagaaatatg
      481 cgcaatgctg cgatatcgtg acatttaatg ccgatctcta tttactcctg tgccgggaaa
      541 atgtacagac gtttacccgt aagatagcga cggtaaacga ttttgacagc agttttggct
      601 accgcaatct gcgcatccat aagtctgcta tttgtgaacc tttgcagggg gaaaacgcct
      661 ggagttacgc agaaaagctg aaactggcga tttccagtat ccgcaaccat atgttctcag
      721 agtttatttt ctgtgatgat gcgaaactca acgaaataga agagaatatc tggattgcgc
      781 gtaatattcg ccatgcaatg gaaattggcg aactattcct ggtctatcaa ccgattgttg
      841 atattaacac ccgcaccatt ttgggcgcgg aggcgttgtg ccgttgggtg tctgcggagc
      901 gggggattat ttcaccgctg aagttcatta ccattgctga agatatcggg tttatcaatg
      961 agctgggcta tcaaattatt aaaaccgcga tgggtgaatt cagacatttt agtcagcgtg
     1021 cgtcgctgaa ggatgatttc ttactgcata ttaatgtttc gccctggcag ttaaacgaac
     1081 cacactttca tgagcgtttt actaccatca tgaaagaaaa tggcctgaag gcgaacagcc
     1141 tttgtgttga gatcaccgaa accgtgatcg agcgaattaa tgaacatttc tatctcaata
     1201 ttgaacaact gcgtaaacaa ggggtacggg tatcgattga tgactttggc accggtttgt
     1261 caaacctgaa acgtttttat gaaattaatc cagacagtat aaaggtggat tcgcaattta
     1321 ccggcgatat tttcggtact gcaggaaaaa ttgtgcgcat tattttcgat ctggcacgct
     1381 ataaccggat cccggtgatt gcggaaggcg tagagagcga agacgttgcg cgtgaattaa
     1441 tcaaattagg atgtgttcag gctcaggggt atctgtacca gaaacccatg ccattctccg
     1501 cctgggataa aagtggaaaa ttagtaaaag agtagtttac gtatgtccag aatcaataaa
     1561 ttcgtactta cagtcagtct gctgattttt atcatgattt cagcagttgc ctgcgggatc
     1621 tacactcaaa tggtaaagga acgggtgtat ggcctgaaac agtccgttat tgatactgct
     1681 tttgcggtgg caaatattgc tgaatatcgg cgtagtgtgg caattgatct gatcaacacg
     1741 ctaaacccta cggaggaaca gctgttggtt ggcttacgca cagcttacgc cgactcggtt
     1801 tcaccctctt atttgtacga tgtcggtcct tatctgattt ccagtgacga atgtattcag
     1861 gtaaaggagt tcgagaaaaa ttattgtgca gatattatgc aggttgttaa gtatcgacat
     1921 gtcaaaaata cagggtttat ctcttttgat ggtaaaacct tcgtctatta cctctatccg
     1981 gtaactcaca atcgtagtct gatatttttg cttggtctgg aacgtttttc tttactgtca
     2041 aaatcgctgg cgatggatag cgagaacctg atgttctccc tgttcaagaa cggtaaatcg
     2101 gtgaccggtg atgaatataa cgctaaaaac gccatcttca ccgtttcgga agcaatggag
     2161 cacttctcct atttgccgac cgggttgtac gtatttgctt ataaaaaaga cgtttatttt
     2221 caggtatgta cattgattat tttctttgcc gcattggtgg cagtgatatc gggtgccagt
     2281 tgcctctatc tggtacgcag agtgattaat cgtggtattg tggagaaaga agccattatt
     2341 aataaccatt ttgaacgcgt actggatggc gggcttttct tttcggctgc cgatgtcaaa
     2401 aaactctaca gtatgtataa ctcggcattc ctggacgatc tgaccaaagc gatgggcaga
     2461 aaatcttttg acgaagattt aaaagcgctg ccggaaaaag gcggttattt gtgcctgttt
     2521 gacgtcgata aattcaaaaa cattaacgac accttcggtc atttgctggg cgatgaagtg
     2581 ttgatgaaag tggtgaaaat ccttaaatca cagatcccgg tagataaagg taaagtttac
     2641 cgcttcggag gtgacgaatt tgcggtgatt tatactggcg gcacgctgga agagttgcta
     2701 tcgattctaa aagaaatcgt tcatttccag gtgggaagca ttaatttaag caccagtatc
     2761 ggtgtagcgc attcaaatga atgtactacc gttgaacgtt tgaaaatgct ggcggatgag
     2821 cggctgtata agagtaagaa aaacggcagg gcgcagatta gctggcagta atcattattc
     2881 gcaggccgga caaatgattt tgcccggcct gaattaatta aacccggcta ccccacaaat
     2941 cgtactcatc ggcgtgctcg actttcactc gcaggatatc acccggctta acgttggttt
     3001 caccattgag ataaaccgcg ccgtcgattt ccggtgcatc tgccatgctg cgaccaatcg
     3061 cgccttcttc gtccacttcg tcgataatca ccagaatttc acggcccact ttctcttgca
     3121 ggcgctcggc ggaaatttgc tgctgcaact gcatgaaacg gttccagcgt tcttctttca
     3181 cttcttccgg aacctggtca ggcagggcat tggcgtctgc accttcaacc gggctgtatt
     3241 taaagcagcc aacgcgatcc agacgtgctt ctttcaggaa gtcgagtagc atctggaaat
     3301 cttcttccgt ctcgccaggg aagccaacaa taaaggttga gcgtagggtc agttccgggc
     3361 agattttgcg ccactgtttg atgcgcgcca gttggcgatc tacagaaccc ggacgtttca
     3421 tcagtttgag aatgcgcggg ctggcgtgct gcaacggaat gtccagatac ggcaggattt
     3481 tgccctctgc catcagtggg atgacgtcgt ccacatgcgg ataagggtaa acgtagtgca
     3541 gacgtgtcca gatccccagt ttcgataact gttcgcacag gctgaccatg ctggttttta
     3601 ccggctcgcc gttgtggaag ccagtacgat gtttaacatc gacgccatac gcggaagtat
     3661 cctgcgagat caccagaatc tctttaacgc ccgcatcaac cagacgtttc gcttcactta
     3721 acacttcgcc aatcggacgg ctcaccaggt cgccgcgcat agacggaata atgcagaagg
     3781 tgcagcggtg attacagcct tcagaaattt tcagataggc ataatgacgc ggtgtcagtt
     3841 tcacaccttg ttctggcacc aggctcagga atgggttgtg tttcggtttt ggcacgtagt
     3901 gatgaacgtg ctccagaacc tgctcatagc tatgaggccc ggtgatttcc agcactttcg
     3961 ggtggacttc gcggatctga tcttcttttg cccccagaca accggtcaca attaccttgc
     4021 cgttttcatt caacgcttca ccaatggctt ccagtgattc ttgtaccgcg ctgtcaataa
     4081 agccgcaggt gttgacgatc accatgtccg catcatcata gctcggtacc acgtcataac
     4141 cttcagtgcg tagttcggtg agaatacgct ctgaatcgac aaggtttttc ggacagccaa
     4201 gggaaacaaa gccgattttc ggctggggag ttactttgct catagcttaa aaaatattca
     4261 gttacaggaa aggtcagggc agggattcta cagagttctg gataaaattt gtatcgcaat
     4321 ctcattcgct ggcggagttg aaggaaatgt aaattttgtt aattcggcgt gaagaattga
     4381 tcccggacag cattttgctc aaaaaacagc catactattt agttgcaaca aggctggaaa
     4441 gaggaggatc gaagtatgtt cgttgacaga cagcgaatcg atatgctgaa ccggttgatc
     4501 gacgcacgcg ttgacctcgc cgcatacgtg caactgagga aggcaaaagg atacatgtcc
     4561 gtcagcgaaa gcaatcatct acgagataac ttttttaaac tgaatcgcga actgcacgat
     4621 aaatcgctgc ggttgaatct tcatctggat caggaagaat ggagtgctct tcatcatgct
     4681 gaagaagcat tagcgacagc cgcagtatgt ttgatgagtg ggcaccatga ttgcccgact
     4741 gttattaccg tcaacgccga taagcttgaa aattgtctga tgagcttaac gctgagtatc
     4801 cagagcctgc agaagcacgc catgcttgag aaggcctgaa aactaagggg gagaaagcgt
     4861 ctcccccttc atgtttaagt ttttgtaaaa atgaatttgt tatctcctcc actgactacg
     4921 ctttaagcca gagtcaatcc ggaggcgtta tgcatcgaca atcctttttc cttgtgcccc
     4981 ttatttgtct ttcttccgct ctctgggcag ctcctgcaac ggtaaatgtc gaagtactgc
     5041 aagacaaact cgaccatccc tgggcgctgg cctttttacc cgataatcac ggtatgttaa
     5101 tcactctgcg cggcggcgag ttgcgtcact ggcaagcagg aaaaggatta tctgcgccgc
     5161 tttccggagt gccggacgtt tgggcgcacg ggcagggcgg cctgctggac gtggttttag
     5221 cgcccgattt tgctcagtcg cgccgcatct ggttaagtta ttccgaagtt ggcgatgatg
     5281 gcaaagccgg aactgctgtg ggttatggcc gcttaagtga tgatctctca aaagtgaccg
     5341 acttccgcac cgtttttcgc cagatgccaa aactgtctac cggcaaccat tttggcgggc
     5401 ggctggtatt cgacggtaaa ggttatcttt ttattgctct gggcgaaaac aatcagcgcc
     5461 cgacggcgca ggatctggat aaattacagg gcaaactggt gcgtctgacc gacctgggag
     5521 aaatcccgga tgataatcct tttataaagg aatctggtgt gcgcgccgag atctggtctt
     5581 atggcattcg taatccgcaa ggaatggcga tgaatccgtg gagtaatgca ctatggctga
     5641 atgaacatgg cccgcgcggt ggcgatgaaa ttaatatccc gcaaaaaggc aaaaactacg
     5701 gctggccgct ggcaacctgg ggaatcaact attcaggctt taagataccg gaggcgaaag
     5761 gggagatcgt cgccgggacc gagcaacctg ttttttactg gaaagattcg cccgccgtga
     5821 gcggcatggc cttctataac agcgacaaat tcccccagtg gcagcaaaaa ttatttattg
     5881 gcgcgctgaa agataaagat gtcattgtga tgagcgtcaa cggcgacaaa gtgacagaag
     5941 atggccgtat tttaacggac agagggcagc gaattcgtga tgttcgcact ggacccgacg
     6001 gttatttata cgttctcacc gacgagtcca gtggggaatt acttaaagtt agcccacgca
     6061 attagctaac gggaatcatc accactttgc gcactgcggg gcgttcagtg agttgctgat
     6121 accagcgttg cagatttgga cgcggtgtcc aggtcaggcc aacgttaaac aaattgtaga
     6181 tgaacggtgc gatagcgata tcgcccacac caaactcgtc gcctgagaac cattttactt
     6241 ttgccagttc cgcatcgagc agggcaaaca gggcgtcgca ctctttgcaa ctggcatcaa
     6301 tggcggcctg atcgcgctct tccggtggtg ttctgactaa tcccatcagg atcccgcgat
     6361 gagcattact gagcgtctgg tttgcccagt ccatccattt ttctgcttcc gcacgacgtg
     6421 ccggtgagtc gatccacagg cgtttttgcc cgtactgtgc cgccagatag cggacaatgg
     6481 cgtttgattc ccaaagaatg agatcacttt cgtcgtcacg caacaacggc accagcccgt
     6541 tagggttcat cgccagaaaa tcagcatcgt gatttatccc aaactcacgg cccgcgagaa
     6601 tttgctcata aggtagttcc agttcttcga gcgtcagcaa tacttttttt acgttggttg
     6661 aattattccg accccacagc gtaatcatac tcaccccttt tccgacaggc agccacaggc
     6721 tgaacaggac tccgatggtg aagtaaagtt aacttttaag caacagctgg caaaaaaatg
     6781 acaccagaag cagcgaagcg gcaggatatt gcataaactt taaaaacttt accaacttac
     6841 ggtttcttta agtttgtgtg tgcgttatta atcaccgaac ttatcatacg gcgatataac
     6901 gtattttttt gaatggatac tcgggtggca tttatgacgc aatactcctc tctccttcgt
     6961 ggtcttgcag cgggttctgc atttttattc ctttttgccc caacggcatt cgcggcggaa
     7021 caaaccgttg aagcgccgag cgtggatgcg cgtgcatgga ttttaatgga ttacgccagc
     7081 ggtaaagtgc tggcagaagg caatgcggat gagaaactgg atcccgcgag cctgactaaa
     7141 atcatgacca gctatgtggt tgggcaggcg cttaaggccg ataagattaa actcaccgat
     7201 atggtgacgg tcggtaagga tgcctgggcg acgggaaatc cggcactgcg tggttcatcg
     7261 gtaatgttcc tcaaaccggg cgatcaggtt tcggtggcag acttgaacaa aggtgtgatt
     7321 atccagtccg gtaatgacgc ctgtattgcg ctggctgatt acgttgccgg gagccaggag
     7381 tcatttattg gtttgatgaa tggttatgcc aaaaaactgg gtctgaccaa cactaccttc
     7441 cagacggtgc acggcctgga tgcgccgggg cagttcagta ccgcgcgcga tatggcattg
     7501 ctgggtaaag cattgatcca cgatgtgccg gaagagtacg ccattcataa agagaaagag
     7561 ttcaccttca acaaaattcg tcagcctaac cgtaaccgtc tgctgtggag cagcaatctg
     7621 aatgttgatg gcatgaagac aggaaccacg gcaggcgcgg gatataatct ggttgcttcg
     7681 gctacccagg gcgatatgcg tttgatctcc gtagtattgg gggcgaaaac tgaccgtatc
     7741 cgttttaatg agtctgagaa attattgacc tggggtttcc gcttctttga aaccgtgacg
     7801 ccaattaaac ctgatgccac ctttgtgact cagcgcgtct ggtttggtga taagagcgaa
     7861 gtgaatctcg gggcaggcga agcgggctca gtgaccatac cgcgtgggca gctgaaaaac
     7921 ctgaaagcga gctacacgtt aacggaaccg cagcttaccg caccgctgaa aaaaggtcag
     7981 gttgtcggga ccattgattt ccagcttaac ggtaaatcca ttgagcagcg tccgctgatc
     8041 gtgatggaaa atgtggaaga gggcggattc tttggtcgga tgtgggattt cgtgatgatg
     8101 aaattccatc agtggttcgg cagctggttc tcttaatttt gtgataaccg gatggcgcga
     8161 aacgtcatcc ggttatacgt cattaataca tcaacttaat acgctgcgtc tgcgcgtact
     8221 tcacatactc atcttccggg caacaatcgc tcaccacaat atcaaagcgt ttcaggtcac
     8281 ccatgcgtgc cggacgcacc ttgccaaatt tactgtggtc gacaaccagc acatgctttt
     8341 gcgccatcga catggcccag tgttttaccg gcaactcttc aagattaaaa caggtagcgc
     8401 ccttactgac atgcaccccc gccgcggaat aaaaagcgat atccgggcaa aaattattca
     8461 gcgtttgctg aaaatcgatg ggtttgaaaa tggcgttgct ggcgtgaaat tcaccaccgc
     8521 aaagaaacgc gcggcaatgg ggtttctctt tcagtgccag aaaggtattt agcgaataac
     8581 aaacggcggt gaaagggatt tcattatcaa tcgcttcaat aatccacggt gtggtggtgc
     8641 cacagtcaaa aaagagggtc tgatcgggtt ctaccagcgt cgcagccagt tttgccgccc
     8701 ggcgtttttc ttccaccagg cgggattttt gatcgcttaa caggtaatgg ctggcgctgc
     8761 gcggttccag aacaatatag ccgccgagca aaacgactgg cgcactgtgg ttgttcagat
     8821 cgcgacgaat cgtcatctcc gaaaccccaa gcagggcggc ggcgtcttta agatgtaact
     8881 tatcactgcg ttttaattct tgcagcagct gcccgatacg ctcttcgcga cgtgtttcca
     8941 taatccctct gaatagttat tgaagcgagc cgctcaatac tacacttttt agcagagatc
     9001 agtcacgcac ccagcctttg cggatcggta atgcaaaaca gaagcgatac cacgattgca
     9061 gacgttgata gagtttatgc cccatcgctt gccagataat ctgggcgcta aggcagccaa
     9121 tcatacctgc cagcaatcca ccgagcatat ccagcggcca gtggacgcca agataaacgc
     9181 gcgaccaggc aatgacgacg gccagcacca ttaaaagtga gccggaccac aggcgatgcc
     9241 agcataaaaa tgccagtgca aaggtgaaaa tcaccgtacc gtgatcgctc gggaatgagt
     9301 catccgccgc atgatgcagg aagttatagc cgatattttc gacaaagggt cggtcgtgcg
     9361 gaaaaagatg tcccatcgtc caggacacaa acaggctgac cgccagcgcg atagcgattt
     9421 ttatcaccag ttgccgttgt gctgtaagcc cccacaacca aagtactgcg gccagcaacg
     9481 gcaccacggt aatcaaatct ttagcaataa aaatcgccaa cgagatcatc cacggcgcag
     9541 agtctggcgt tgcgttaata agagagaata gagagagatt caaattttcc agcataactt
     9601 cccgacgcaa agtgattaaa agggagccaa tacaggcaag tcgttgagaa taaagtgcag
     9661 gttaaactgg gtaaagcggc atcgtcttat ttccctcaag cggcctgttt acggtaggtg
     9721 attgtaacgg gcaaaggtta aataaaactt aaagaaagcg tagctatact cgcaataatg
     9781 taagaatgtg cttaaccgtg gtttcagcta caaaattcgc tttctcgtta gctgcgcttt
     9841 tattaaactt tgcgcgatta ttattggcga agaaattgca tgcaaaataa attagcttcc
     9901 ggtgccaggc ttggacgtca ggcgttactt ttccctctct gtctggtgct ttacgaattt
     9961 tcaacctata tcggcaacga tatgattcaa cccggtatgt tggccgtggt ggaacaatat
    10021 caggcgggca ttgattgggt tcctacttcg atgactgcgt atctggcggg cgggatgttt
    10081 ttacaatggc tcctggggcc gttgtcggat cgtattggcc gccgtccggt gatgctggcg
    10141 ggagtggtgt ggtttatcgt cacctgtctg gcaatattac tggcgcaaaa cattgaacaa
    10201 ttcaccctgt tgcgcttctt gcagggcata agcctctgtt tcattggcgc tgtgggatac
    10261 gccgcaattc aggaatcctt cgaagaggcg gtttgtatca agatcaccgc gctgatggcg
    10321 aacgtggcgc tgattgctcc gctacttggt ccgctggtgg gcgcggcgtg gatccatgtg
    10381 ctgccctggg aggggatgtt tgttttgttt gccgcattgg cagcgatctc ctttttcggt
    10441 ctgcaacgag ctatgcctga aaccgccacg cgtataggcg agaaactgtc gctgaaagaa
    10501 ctcggtcgtg actataagtt ggtgctgaag aacggccgct ttgtggcggg ggcgctggcg
    10561 ctgggattcg ttagcctgcc gttgttggcg tggatcgccc agtcgccgat tatcattatt
    10621 accggtgagc agttgagcag ctatgaatat ggcttgctgc aagtgcctat tttcggggcg
    10681 ttaattgcgg gtaacttgct gttagcgcgt ctgacctcgc gccgcaccgt acgttcgctg
    10741 attattatgg gcggctggcc gattatgatt ggtctgttgg tcgctgctgc ggcaacggtt
    10801 atctcatcgc acgcgtattt atggatgacc gccgggttaa gtatttatgc tttcggtatt
    10861 ggtctggcga atgcgggact ggtgcgatta accctgtttg ccagcgatat gagtaaaggt
    10921 acggtttctg cggcgatggg gatgctgcaa atgctgatct ttaccgtcgg tattgaaatc
    10981 agcaaacatg cctggctgaa cgggggcaac ggactgttta atctcttcaa ccttgtcaac
    11041 ggcattttgt ggctgtcgct gatggttatc tttttaaaag ataaacagat gggaaattcg
    11101 cacgaagggt aaaaaaatgc ctgattgctt tgtgcgatca ggcattctcg aattaatggt
    11161 gatggtcgtc aatctggtgt tcgataacca tcccttcacc tacgctggca agatggcgaa
    11221 cataaggatg cgggcggtaa gccggagctg gcgcaggagc cacatagacg gtttgcggca
    11281 cagtcgcgac gctgaccgct tgtggaacgc tgactgaatc aggaaccaca accactttgt
    11341 atccactcgg cacatcgacg gtgacacttt gcgccatcgc cacaccagta aagcccatta
    11401 aaagcgcgcc caacagtaga caatttttca taatcatttc gcctgaaata atgcagcggg
    11461 ttaatttccg cgaattatgc aatcttcatt caggcattgt gttgccgggc gtgctgccgt
    11521 tttattggtt aaatggcgct tcgtgtttaa gcactttatc gatcacatcc agtacgcctt
    11581 cacggttatt ggagcctgcc cggtattttg ccgctgcgac gaccgcgctg ccggcatttt
    11641 ccattgcaaa actaaagcct gcctgacgca gcatctcaat atcgttaccg ccatcgccaa
    11701 agaccaccac ttcgctgtcg tctattcccc ataatttctg cagttggcga aggccattgg
    11761 ctttatgtac accggggata atcagatcga tgctgccgtt gccggtatgg accggtacca
    11821 tgatatcgcc gatggcctca tgtaatgctt tttgcacttg tgggatcagt tcgtcagaga
    11881 gattaaggcc aaatttaaag aagatatctt ctaagttgtc aaagttatcg acgtattcca
    11941 gacggtgata atacatttcc gccaccgttt tcatggcatc gtcatatttt ttgagggtat
    12001 aggcgctatt ttttccgcag gcaataattt ccacttccgg gcgcgtcagc aaatgttcta
    12061 cgacagtagt aaacgcatcc ttcgacagct cgccattaaa aacatctttg ccttcgctca
    12121 ctacccagcc gccgttttca gccacaaagg caatttcatt agcaatttca ggaaagaaag
    12181 agatcaactg ataatattga ttcccgctgg cgaccacaaa gcgaatgcct tgtgctttca
    12241 tttgctgata ctgagccata aaccgctcac ggttataggt tttttgatcg cttaagaaag
    12301 taccatccat gtctaccgca attaatttaa tgctcatcaa ctattctcca tcgccgtctg
    12361 cgttttggta tcgggtttgg cgacggcttt cgcgacaata gccgcgagaa taaccagcgc
    12421 cagtacaacc agcattgcac tacgtaatcc ataatgttcg ccgagatagc ccagcagcgg
    12481 cggcccgacg aggaaagcca gataaccggt cgtagctacc acgctgacgc gggttggtgc
    12541 atcggggccg gtatcgctgg cggcagaaat agtcagcggg aagcccaacg aggcacccag
    12601 tccccaaagt acaacagaca ccccagcgac ccaggcgcta tcgacaaaaa taatcagccc
    12661 aatacccaac gcccccatta gtgcactggc ccgaaccacg gcaacgcgac tgtaacggtc
    12721 aatgaaccaa ccgccggtaa agcgtccaac ggtcatcccc agggtaaaac cggcataaat
    12781 cagcgagccg gaagtagggc taaaaccgtg accatcaacc attaataagg gtaaccagtc
    12841 gttggcagaa ccttcggcaa aggccatcgc cagcaccaca acaccaatca acagcaactg
    12901 gatatcccga taaaaaggta cgcctttttc accatgctgg gtgccatcgg cagcattttt
    12961 gcccgtaccg tcagggattg cctgaatggc gatataaata ggtgcgatgc ctaccagcgc
    13021 cgccaataaa atatgcaccg ttgccggaac gccaaaggcc gtaagtgcca tcccgacacc
    13081 tgcgcctgcc agcgtgccca ggctataaaa accgtgcatc atcggtaaaa ccgtcttatt
    13141 catttctctc tcaacggcgg caccttcaac gtttatcgcc acttccgcag aaccaaaact
    13201 agccccaaag acgccgagac caacggcaaa gagcaagggc gatgtcagcc agagtgccag
    13261 acttaatatc atcatcccaa tcaatgcgca ggacatcgtc acaaggatga cattacgcgt
    13321 cccaaagcgt ttcactaacc acgccgagca gagaataccg ctcatcgaac cgatcgacag
    13381 accaaagaga acaccgccca tttcagcgat cgagacagag agaatatcgc ggatagcagg
    13441 cgtacgggtt gcccaggacg ccattaacag gcctggcaaa aagaagaaca taaacagcgc
    13501 ccaggttcgg cgtttcaatg cattacgtga agaatttacg gtcatagacc acgtcaaaat
    13561 aagaagaggg aagacaacat tagcaagact gtgtacattt gtacacaatt gcagagagag
    13621 gaaatgacat gcgtcgcgct aacgatccgc aacggcgaga aaaaattatc caggccacgc
    13681 tggaggcggt gaaactttac ggaatacatg ctgttacaca ccgcaaaatt gctacccttg
    13741 ccggggtgcc gttgggatcg atgacctact atttttcagg aattgatgag ttgttgctgg
    13801 aggcgttcag ccgttttact gagatcatgt cccggcaata tcaggcattt tttagcgatg
    13861 ttagtgatgc tccaggcgca tgccaggcta tcaccgatat gatctacagc tcacaggtcg
    13921 caacgccgga taatatggag ctgatgtacc agctctacgc gctggctagc cgaaaaccgc
    13981 tcttaaaaac ggtaatgcaa aactggatgc agcgcagtca acaaacgctc gaacaatggt
    14041 ttgaacccgg aaccgcccgc gcgcttgatg cgtttattga gggaatgacg ctgcattttg
    14101 tcaccgaccg taagccgcta tcacgcgagg agattttgag gatggttgag aggattgctg
    14161 ggtagtagat aaatttcagg caacaaaaaa cccatcaacc ttgaaccgaa atggcggggt
    14221 tgatgggctc cacaaaatgg ggacatcaaa gaaaagcagt ggcactaatt aagactgatg
    14281 ccctgcggaa aagttctgcg gttgtgcaaa aaaaatttca ttttcagggc aacttcagtt
    14341 ttatcctaat cctggccata ccatgacgat gattgtccct gccagcgtca gcaggacgtt
    14401 ggcgattgca taggtgcccg catagcccag cgccgggatg ttactgcgag ctgtatcact
    14461 gatgatctcc attgccggcg cgcaggtgcg tgcgcccatc attgcgccga ataacagtgc
    14521 gcggttcatt cgcaatacat aagcaccgaa caagaaacag ataaccactg gcacaagact
    14581 gacaatcaat ccggcaatca acatctgacc gccaatcgcg cccaggccgt tattaatacc
    14641 gctaccggcg ctcagaccaa cgcctgccat aaacaccatt aagccgaact ctttcaccat
    14701 gcttaatgca ccctgcggaa tgtaaccgaa ggtcgggtgg ttagcgcgca taaagcccag
    14761 cataattccg gcgaataaca acccggcagc gttccccatg ccgaaactga atgtgctgaa
    14821 ctggaaggtg atcatcccga tcatcagccc aataacaaag aaggcgcaga atgccagcaa
    14881 gtcagtgacc tggctgtgaa tcgagataaa gccgatgcga tcggcgatgg tttttacgcg
    14941 acgggcatcg ccgctgactt gtaaaacgtc acctttgtta agcacgacgt tgtcatctat
    15001 cggcatctca atctggctac gaatgacgcg gttaaggaag caaccgtgat cggtcaactt
    15061 cagttgtgcg agacgtttgc caacggcgtt atggttttta acgaccactt cttcagtgac
    15121 gatacgcatg tcgagaaggt cacgatcgaa aacttctttg ccgttacgga agctgggatc
    15181 gagtcgtgcg tgggcgtcgg gatagcctac caacgctatt tcatcgccca tttgtagcac
    15241 agcatcaccg tctggatttg ccagaatccc gttacgtcga atacgttcaa tgtagcagcc
    15301 agtttgtcga taaataccca gttcacgcag atttttgccg tcggtccagg ccaccagctc
    15361 cgggccgacg cggtaggcgc ggatcaccgg taaataaacc ttacggttgg catcagtgtc
    15421 caggccacgt tcgcgggcga tttgctgggc gctggtctgt aagtcctgat gctgcaattt
    15481 cggcaagtaa cgcgcaccaa caatcaaact caccagaccg attaaatagg ttaaggcata
    15541 cccgaggctc agattatcca gtgccagtga gagctgcctg ctttccatgc cggaatgacg
    15601 cagtgtatcg ccagcaccga ccagaaccgg tgtcgacgtc atagagccag ctaacatacc
    15661 ggccgtcagg ccaatatccc agccaaacag cttacctaac cctaaggcga tcaccagcgc
    15721 actgccaacc atcaccagtg ctaacattag gtaatttttc ccatcgcgaa aaaaaatgga
    15781 aaaaaagttc ggtccggctt cgaccccgac gcagaaaata aacagcataa agccaagatt
    15841 aagcgcgtcg gtgttaatgc tgaaatgttg ttggcctaat aacagcgata cgactaaaac
    15901 gccaatggaa ttacccagtt ggatcgaacc aagtcgtaat ttcccgagac atagcccaag
    15961 cgcgaggacc acaaataata acagaatgta attcccattt aacaattcgg cgacgtttat
    16021 attcacggag gctaacttct tgtttactag taagctgttg aaagaaatgg taatttacga
    16081 taatgttttt taccagaatt cagggcgcag attcattcag cgcacctaaa cgatagtaaa
    16141 gtaacaatat attttactag tgtaatcaca ttaggtatca acggctatat gaattgcgtt
    16201 ggcctatatt agcatggaat gcgaagcggc tttatcttac tgaacgccac actggcgaaa
    16261 aatgtgttcg atagacgcag tgtcaggagg aacgagtgaa acataaacaa cgttgggcgg
    16321 gggcaatctg ctgttttgtc ctcttcattg tggtgtgtct ttttctggcg acgcacatga
    16381 aaggcgcttt tcgggctgcc gggcatcctg aaatcggttt gctatttttc attcttcctg
    16441 gtgcagttgc cagcttcttt tcacatcgta gagaagtcct gaaacctctg tttggcgcaa
    16501 tgctggcggc accctgttcg atgctcatta tgcggctgtt tttttcaccg acgcgctcat
    16561 tctggcaaga gctggcatgg ttattaagtg cggtgttctg gtgtgcgctg ggggcattgt
    16621 gtttcttatt tatcagtagt ttgtttaaac cacagcacag aaaaaatcaa taaagccctc
    16681 aacgcgaggg cttgtcagac gatcaggcgt ccagattctc tttcacccat gcagcaaaat
    16741 cggtatagcc gccgatatgt tgctgatcga caaaaatctg cggcacggtt tctacgggtt
    16801 tacctgcctt ttgttgtaga tcttctttag tgatcccttc cgcacgaata tctacatact
    16861 gatactggaa atcatcgcgt tcattgctca atttctcagc cagatctttt gcacgcacac
    16921 agtaagggca acccggacga ccaaaaataa cggtttgcat tatttctctc ctcatagatt
    16981 tatgcctgta atcatcacgc taaaatgtat tcgctgaaag taggtttaac ctgttgcatt
    17041 aattgataaa agctataact gttaaacaca atacagtgaa aagttttaga ctgaaggctc
    17101 actttgcaga gggaagcgta tgcgcgcgat cggtaaattg cctaaaagcg tgttgatact
    17161 ggaatttatc ggaatgatgt tgctggcggt ggcgctgctg tcggtaagcg actccctgtc
    17221 gctacctgag ccattttctc ggccagaagt gcagattctg atgatttttc tcggtgtttt
    17281 gctcatgctt cccgctgcgg tggtggttat tcttcaggtg gcaaaacgtc ttgccccaca
    17341 gctgatgaac catccaccgc aatattcacg ttcagaaaga gaaaaagata atgacgccaa
    17401 ccattgaact tatttgtggc catcgctcca ttcgccattt cactgatgaa cccatttccg
    17461 aagcgcagcg tgaggcgatt attaacagcg cccgtgcgac gtccagttcc agttttttgc
    17521 agtgcagtag cattattcgc attaccgaca aagcgttacg tgaagaactg gtgacgctga
    17581 ccggcgggca aaaacacgta gcgcaagcgg cggagttttg ggtgttctgt gccgacttta
    17641 accgccattt acagatctgt ccggatgctc agctcggcct ggcggaacaa ctgttgctcg
    17701 gtgtcgttga tacggcaatg atggcgcaga atgcattaac cgcagcggaa tcgctgggat
    17761 tgggcggggt atatatcggc ggcctgcgca ataatattga agcggtgacg gaactgctga
    17821 aattaccgca gcatgttctg ccgctgtttg ggctgtgcct tggctggcct gcggataatc
    17881 cggatcttaa gccgcgttta ccggcctcca ttttggtgca tgaaaacagc tatcaaccgc
    17941 tggataaaga cgcactggcg cagtatgacg agcaactggc ggaatattac ctcacccgtg
    18001 gcagcaataa tcgccgggat acctggagcg atcatatccg ccgaacaatc attaaagaaa
    18061 gccgcccatt tattctggat tatttgcaca aacagggttg ggcgacgcgc taaaactgcc
    18121 acgtcgatgt atgatacgcg ggcttttgac caggtctgat agagaggtgc agagtgaaaa
    18181 ttgccatatt gtcccgggat ggaacgctct attcgtgtaa gcggctgcgt gaagccgcta
    18241 tacagcgcgg tcacctggtt gaaattcttg atccgctttc ttgctacatg aacataaatc
    18301 ctgcggcgtc ttctattcac tacaaaggcc gcaagttacc ccattttgac gcagtgatcc
    18361 cgcgtattgg caccgccatt accttttatg ggacggcggc actgcgccag ttcgagatgc
    18421 tggggagcta tccgctcaat gagtcggtcg ccattgcccg ggcgcgtgac aaattgcgtt
    18481 ccatgcaact gctggcgcgt cagggcatcg acctgcctgt cacgggcatt gcgcattcgc
    18541 cggatgatac cagcgattta atcgacatgg tcggtggtgc gccgctggtg gtcaagctgg
    18601 ttgaaggcac gcagggaatt ggtgtcgtgc tggcggagac gcgtcaggcg gcggaaagcg
    18661 tgattgacgc tttccgcggt ctgaacgcgc atattctggt gcaggaatat atcaaagagg
    18721 cgcaagggtg cgatatccgc tgtctggttg ttggcgatga agtagtcgct gcgattgaac
    18781 ggcgagcgaa agagggcgat tttcgttcca atttgcatcg tggcggcgcg gcaagcgtcg
    18841 ccagtatcac accacaggag cgtgaaatcg cgataaaagc cgcgcgaacg atggcgctgg
    18901 acgttgctgg tgtggatatt ctgcgtgcta atcgcgggcc gttggtgatg gaagtgaatg
    18961 cgtcgccagg gctggaagga atagaaaaaa ccaccggtat cgacatcgcg ggtaaaatga
    19021 tccgctggat cgaacgccac gctacgacag aatattgcct gaaaacgggt ggttagtcgc
    19081 aatcacatta ctgatcatgg ttttgcctgc gctttttgcg taagctgtgc cggtcttttt
    19141 atcggaagag gttgtacaaa attatgacat cgctggtcgt tcctggtctg gatacgttgc
    19201 gtcaatggct cgatgacctg gggatgagtt tttttgaatg tgataactgt caggctctgc
    19261 atctgcccca tatgcagaat ttcgacggtg tctttgatgc taaaatcgat ctgatcgata
    19321 acacgatcct gttttctgcc atggcggaag tccgaccttc agccgtattg ccgctggcgg
    19381 cggatttatc agccatcaat gccagttcgc tgaccgtgaa agcatttctt gatatgcagg
    19441 atgataatct gccaaagctg gtggtttgcc agtctttatc cgttatgcag ggcgtaacct
    19501 atgagcagtt tgcatggttc gtgcgtcaga gcgaagagca gatttcgatg gtcattcttg
    19561 aagctaatgc ccatcaactg ctgttaccga ctgacgatga agggcaaaat aacgttaccg
    19621 aaaactattt cctccactga taactccttt cgagcacgca gtcgctgccg cagtgactgc
    19681 gtgccgcaaa attatccgct gtttttaacc ttttattaaa gattaattca cttctcttgt
    19741 gc
//
LOCUS       DAZFJL010000087        19694 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZFJL010000087 DAZFJL010000000
VERSION     DAZFJL010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.087"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            95..637
                     /gene="yfaZ"
                     /locus_tag="ACHJ8C_003055"
     CDS             95..637
                     /gene="yfaZ"
                     /locus_tag="ACHJ8C_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO5552871.1"
                     /translation="MKKIALAGLAGMLLISASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNDGDEGYAAAVGGG
                     LQWQIGDSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLAGK
                     NGNRDNAIADGPYVGVNASF"
     gene            737..1939
                     /gene="yfaY"
                     /locus_tag="ACHJ8C_003056"
     CDS             737..1939
                     /gene="yfaY"
                     /locus_tag="ACHJ8C_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO5552872.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAIQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEEQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQSRQFSLTLSEQF
                     TGGLLALQLSRADAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGLEDEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2159..2941
                     /gene="yfaX"
                     /locus_tag="ACHJ8C_003057"
     CDS             2159..2941
                     /gene="yfaX"
                     /locus_tag="ACHJ8C_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO5552873.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWERATPTTITHPQRLYEELARIRRRGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            2956..4186
                     /gene="rhmD"
                     /locus_tag="ACHJ8C_003058"
                     /pseudo
     CDS             2956..4186
                     /gene="rhmD"
                     /locus_tag="ACHJ8C_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            4243..5532
                     /gene="yfaV"
                     /locus_tag="ACHJ8C_003059"
     CDS             4243..5532
                     /gene="yfaV"
                     /locus_tag="ACHJ8C_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5552874.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKKLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLTFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFINLFGAVGGFIAPILRVKAETLFS
                     SDAAGLLTLAAVEVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5550..6353
                     /gene="yfaU"
                     /locus_tag="ACHJ8C_003060"
                     /pseudo
     CDS             5550..6353
                     /gene="yfaU"
                     /locus_tag="ACHJ8C_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
     gene            complement(6394..6603)
                     /locus_tag="ACHJ8C_003061"
                     /pseudo
     CDS             complement(6394..6603)
                     /locus_tag="ACHJ8C_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6604..7575)
                     /gene="rpnE"
                     /locus_tag="ACHJ8C_003062"
     CDS             complement(6604..7575)
                     /gene="rpnE"
                     /locus_tag="ACHJ8C_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO5552875.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSEGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGEASPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLEGLQQGKLEGLQ
                     KGKLEGLREGMHEQAIKIALRMLEQGFEREIVLATTQLTDADIPNCH"
     gene            complement(7768..8958)
                     /gene="glpC"
                     /locus_tag="ACHJ8C_003063"
     CDS             complement(7768..8958)
                     /gene="glpC"
                     /locus_tag="ACHJ8C_003063"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO5552876.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(8955..10214)
                     /gene="glpB"
                     /locus_tag="ACHJ8C_003064"
     CDS             complement(8955..10214)
                     /gene="glpB"
                     /locus_tag="ACHJ8C_003064"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO5552877.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTEIHSGLESLRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLKVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWHWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHVAQQIAQRAGGQQ"
     gene            complement(10204..11832)
                     /gene="glpA"
                     /locus_tag="ACHJ8C_003065"
     CDS             complement(10204..11832)
                     /gene="glpA"
                     /locus_tag="ACHJ8C_003065"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO5552878.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGINAEAIEPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGEIQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEATLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12105..13463
                     /gene="glpT"
                     /locus_tag="ACHJ8C_003066"
     CDS             12105..13463
                     /gene="glpT"
                     /locus_tag="ACHJ8C_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO5552879.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13468..14544
                     /gene="glpQ"
                     /locus_tag="ACHJ8C_003067"
     CDS             13468..14544
                     /gene="glpQ"
                     /locus_tag="ACHJ8C_003067"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO5552880.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            14747..15397
                     /gene="inaA"
                     /locus_tag="ACHJ8C_003068"
     CDS             14747..15397
                     /gene="inaA"
                     /locus_tag="ACHJ8C_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO5552881.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(15451..15705)
                     /gene="yfaE"
                     /locus_tag="ACHJ8C_003069"
     CDS             complement(15451..15705)
                     /gene="yfaE"
                     /locus_tag="ACHJ8C_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO5552882.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(15705..16835)
                     /gene="nrdB"
                     /locus_tag="ACHJ8C_003070"
     CDS             complement(15705..16835)
                     /gene="nrdB"
                     /locus_tag="ACHJ8C_003070"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO5552883.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(16924..19209)
                     /gene="nrdA"
                     /locus_tag="ACHJ8C_003071"
     CDS             complement(16924..19209)
                     /gene="nrdA"
                     /locus_tag="ACHJ8C_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO5552884.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4531 a         4857 c         5356 g         4950 t
ORIGIN      
        1 agatcccttt tttcgctcat tgaatcctct gcgccaggca gtacaatccc gcagctttgg
       61 gaattttgat taattgcttg tgggaaaggg gataatgaaa aaaattgcgc tagcaggtct
      121 ggccggaatg ctgttgattt ctgcatcggt caatgcaatg agcatcagcg gccaggcggg
      181 taaagaatac accaatattg gtgtcggttt tgggactgaa tcgacgggcc tggcgttaag
      241 cggtaactgg acacataacg acgacgacgg tgatgtcgcg ggcgtggggc tggggttgaa
      301 tctgcctctc gggccattaa tggcgaccgt tggtggtaaa ggcgtttaca ctaacccgaa
      361 cgatggtgat gaaggttatg cagcggcagt tggcggtggt ttgcagtggc aaattggtga
      421 cagcttccgc ttgtttggtg agtattacta ctctccggat tcgctctcca gcggaattaa
      481 aagttatgaa gaagcgaatg ctggcgcgcg ttacaccatt atgcgtccag tcagcattga
      541 ggctggctac cgttacctga acctggcagg taaaaatggc aatcgtgata acgccattgc
      601 tgatggtccg tatgttgggg ttaacgccag cttctgacgc cttccggcgc ggcgttctgt
      661 cgcgccattc agtttcttac tacgtcctgt ctccctttgc gttatagtga ttcgataaca
      721 aaagcaggag ataaccatgt taaaagtgga aatgttatcc accggggatg aagtgttaca
      781 cgggcaaatc gttgacacta acgctgcctg gctggccgat tttttctttc atcaggggct
      841 gccattatct cgccgcaata cggtggggga taaccttgat gacttagtca ccattcttcg
      901 cgaacgtagt cagcacgccg atgtgctgat cgttaacggc gggctgggac cgaccagtga
      961 tgatttaagc gcactcgccg ctgcgacagc aaaaggtgaa ggcctggtgc tgcatgaagc
     1021 ctggctcaaa gagatggaac gctatttcca tgagcgggga cgagtaatgg caccgagcaa
     1081 ccgtaaacaa gcggagttgc ctgccagtgc tgaatttatt aataacccgg taggcactgc
     1141 ctgtggtttt gccatacagc ttaatcgttg cctgatgttc tttacccccg gcgtaccgtc
     1201 agaatttaag gtgatggtcg agcacgaaat cctgccgcgc ctgcgcgagc gtttttcttt
     1261 accacagccg ccggtttgcc tgcgtttgac tacctttggt cgttcggaaa gcgatctggc
     1321 acaaagcctg gacactctac aactgccgcc gggcgtaaca atgggctatc gctcctcaat
     1381 gccgattatc gaactgaaac tcaccggacc ggcaagcgag gaacaggcga tggaaaaact
     1441 gtggctggac gttaaacgtg ttgccggaca gagcgtgatt ttcgaaggca ccgaaggact
     1501 gcccgcgcag atcagtcgcg aattgcagag ccgccagttc agcctgacgc tgagcgagca
     1561 attcaccggt ggtttattgg ctttgcaact ttctcgcgca gatgctccat tgctggcgtg
     1621 tgaagtggtt ccttcacagg aggaaaccct ggcgcaaact gcgcactgga ttacagaacg
     1681 gcgggcgaac cattttgctg ggttggcact ggctgtttct ggtctggaag atgagcatct
     1741 caactttgca ctagccacgc cagacggcac tttcgctttg cgtgtgcgtt tcagcactac
     1801 gcgctacagt ctggctattc gccaggaagt ctgcgcaatg atggcgctta atatgctacg
     1861 ccgttggtta aacggccagg acatcgccag cgagcatggc tggattgagg ttgttgagtc
     1921 catgacctta tccgtctgat tccgccactc tgtgccggtt tactgcaccg gcacattccc
     1981 cctccggcaa aatttgtcca acctgttgta ctcaataaga aaaaatccgt gcgctgattc
     2041 acaaatttgt gcgtgatttc tccagtccgc tggacgagga gcgggatttg atgcgcttca
     2101 caattttact ccccctgagc aacgagactg attccagtat ctaagactgg agtccagtat
     2161 gttggaatca agcaaagttc ccgccctgac acgggctatt gatattctca atttgattgc
     2221 ccgcattggc ccctgtagtg ctgcgacgat cattgacaca ttggggatcc ctaaaagcac
     2281 agcctatttg ctgcttaatg aactcaggcg tcagcgtttt cttagccttg atcaccagga
     2341 aaatttttgt ttgtggacca ggctggtgga gctttccggt catgcgttaa gcaagatgga
     2401 tctccgggaa ctggctcgac cgcgtttgac gcaactgatg gataccaccg ggctgttatg
     2461 tcacctgggg attattgata acggcagcgc ttattacatt ttgaaagtgg agtcatcagc
     2521 cactatcagc gtgcgttccc atgaaggaaa aagcctttcg ctttatcgct ccggaatagg
     2581 taaatgcctg cttgcctggc aacctgcagc tgtacagcaa agtattatcg aagggctggt
     2641 ctgggaacgg gcgacaccca caacgattac tcatccacaa cggctctacg aagaactggc
     2701 gcgtattcgc cgccggggct ggagctatga caacggcgaa gattacgccg atgtccgttg
     2761 tgtcgctgcg ccggtgttta acgccaataa cgagcttacc gctgcgattt cggtggtcgg
     2821 tacccgctta caaatcaatg aagaataccg tgattatctg gcgggcaagg ccattgcctg
     2881 cgccagggat atttcacgtt tgttgggatg gaaaagtccc tttgacttac aggcctcata
     2941 acaatggaga acatcatgac cctaccaaaa atcaaacagg ttcgcgcctg gtttactggc
     3001 ggtgcgacag cagaaaaagg cgctggcggc ggtgattatc acgaccaggg ggcgaatcac
     3061 tggattgatg atcatattgc caccccgatg agtaaatacc gcgaatacga gcagtcccgc
     3121 cagtcatttg gcattaacgt tcttggcacg ttgattgttg aagttgaagc agaaaacggc
     3181 cagaccggat tcgctgtttc gacagccggt gaaatgggct gtttcgacag ccggtgaaat
     3241 gggctgtttt atcgtcgaaa aacaccttaa ccgtttcatt gagggtaaat gtgtcagtga
     3301 tatcaaactg atccacgatc aaatgctcaa tgccaccctg tattactccg gctctggtgg
     3361 cctggtgatg aatacgattt cctgtgtcga tctggctctg tgggatctgt tcggcaaagt
     3421 ggtcgggctt ccggtttata aacttttagg tggcgcagtt cgtgatgaaa ttcagttcta
     3481 cgccacaggt gcgcgtccgg atctggcaaa agagatgggc tttattggtg gcaaaatgcc
     3541 gacgcactgg gggccacatg atggtgatgc ggggatccgc aaagatgccg ctatggtcgc
     3601 ggatatgcgt gaaaaatgcg gtgaggattt ctggttaatg ctcgactgct ggatgagtca
     3661 ggacgtgaac tatgccacca aactggccca cgcttgcgcg ccctataacc tgaaatggat
     3721 cgaagagtgc ctgccgccac agcagtatga aggttatcgc gaactgaaac gcaacgcgcc
     3781 agcggggatg atggtcacca gtggtgagca ccacggcaca ctgcaatctt ttcgtacgct
     3841 ttcagaaacc ggtatcgaca tcatgcagcc ggatgttggc tggtgcggtg gcttaaccac
     3901 gctggtggaa attgccgcaa tcgccaaatc cagggggcaa ctggtggtgc cgcacggttc
     3961 gtctgtttac tcccaccatg cggtgatcac cttcaccaat acgccattca gcgaattcct
     4021 gatgaccagc ccggattgtt caacgatgcg tccgcaattt gatccgattc tgcttaatga
     4081 gccggttccg gtgaatggtc gtattcataa atcagtgctt gataaacccg gtttcggcgt
     4141 cgaactcaat cgtgactgta atttgaaacg cccctacagc cactaatcac tccgggcgtt
     4201 gctgtgacaa cgttaacgcc cacatattct aaggagcttt ttatgagcac cgctttgctt
     4261 gacgccgtgg tgaagaaaaa ccgtgcgcgt ttaattccgt ttatgttggc gctgtatgtg
     4321 ctggcgtttc tcgaccgttc gaatattggt tttgccaaac agacctacca gattgatacc
     4381 gggctgagta atgaagctta tgcgctggga gcaggcattt tctttgtggt atacgcgttt
     4441 ctgggtgttc cggcgaatct cttgatgcgc aaactggggg ccagaacctg gattggtacg
     4501 acaacactgc tgtggggatt tctttcggca gccatggcat gggccgatac tgaagcgaaa
     4561 tttctgatcg ttcgcactct gcttggtgct gcggaggctg ggtttttccc tggtatgatt
     4621 tatctcacct cgcaatggtt tccgcagcgt aatcgcgcca gcattatggg gctgttctat
     4681 atgggcgccc cgctggcgtt aacactggga tcaccgcttt ccggcgcgct gctggagatg
     4741 catggattta tggggcatcc cggctggttc tggatgtttg ttattgaagg attgttggca
     4801 gtcggcgctg gggtattcac attcttttgg cttgatgaca caccggagca ggcacgtttt
     4861 ctgagtaaac aagaaaaaaa gttgcttatc aatcaactgg caagtgaaga acaacagaaa
     4921 gtgacttccc ggctgagcga tgcgctgcgt aatgggcgag tctggcaact ggcgattatc
     4981 tatctgacca ttcaggtggc ggtttacgga ttaactttct tcctgccgac ccaggttgct
     5041 gcattgctgg ggacaaaagt gggctttacg gcgtcggtgg tcaccgccat tccgtgggtt
     5101 gcggccttgt ttgggacctg gcttattccg cgctattccg ataaaactgg cgaacggcgt
     5161 aatgtcgcag cgctgacatt actggcggcg ggcattggta ttggtctgtc cgggctgctt
     5221 tctccagtat tggcgatcgt agcgctgtgt gttgcagcca tcgggtttat tgccgtacag
     5281 ccagtgttct ggacgatgcc gacacagctt ctttccggta cggcgctggc tgcggggatt
     5341 ggttttataa acctgtttgg tgcagtgggc gggtttattg ccccgatcct gcgcgtgaaa
     5401 gcagaaacgt tattttccag cgatgcggcg ggattactga cgctggcagc ggtggaggtc
     5461 atcggttcgc tgattatttt cactctgcgt gtaaatcgca ctgttgcgca gaccgacgtg
     5521 gcacatcatt aaatagttta aggaacacga tgaacgcatt attaaccaat ccctttaaag
     5581 aacgtttacg caagggcgaa gtgcaaattg gtctgtggtt aagctcaacg actgcctata
     5641 tggcagaaat tgccgccact tccggttatg actggttgct gattgacggg gagcatgcgc
     5701 caaacaccat tcaggatctt tatcaccaac tacaggcggt agcgccctat gccagccacc
     5761 ccgtgatccg tccggtggaa ggcagtaagc cgctgattaa acaagtcctg gatattggcg
     5821 cgcaaactct actggtcccg atggtcgata ctgccgatca ggcacgtcag gtggtgtctg
     5881 ccacgcgcta tcctccctac ggtgagcgtg gtgttggggc cagtgtggca cgggctgcgc
     5941 gctggggacg cattgagaat tacatggcgc aagtcaacga ttcgctttgt ctgttggtgc
     6001 aggtggaaag taaaacggca ctggataacc tggacgaaat cctcgatgtc gaagggattg
     6061 atggcgtgtt tattggacct gcggatcttt ctgcgtcgtt gggctacccg gataacgccg
     6121 ggcacccgga agtgcagcga attattgaaa ccagcattcg gcggatccgt gctgcgggta
     6181 aagcggctgg ttttctggct gtggctcctg atatggcgca gcaatgactg gcgtggggag
     6241 cgaactttgt cgctgttggc gttgacacga tgctctacag cgatgccctg gatcaacgac
     6301 tggcgatgtt taaatcaggc aaaaatgggc cacgcgtaaa aggtagttat tgatatcaaa
     6361 ggcccatggg gatcggctgt gggcctgtgt taattagtgg ttattcgctg ccagatcggc
     6421 ttcgcttagc tgagtggccg cgagcacctg gtcacgatca atgccctgct ccagcatgcg
     6481 caaagcaatt ttaatggctt gttcatgcat gctttcctgt aaaccttcta atttaccttc
     6541 ttgtcggcca atttgatgtc cttcctgccg tagccgttca gcaatagtca ttaatctctc
     6601 cttttaatga cagttcggaa tatcagcatc agtgagttgg gttgtcgcca gcacaatctc
     6661 acgttcaaag ccctgctcca gcatgcgcaa ggcaatttta atcgcttgct catgcatgcc
     6721 ttcccgcaaa ccttctaatt tacctttctg caaaccctcc aatttacctt gctgcaaacc
     6781 ttctaattta ccttcctgcc agccaatttg atgtccttcc tgccgtagcc gttcagcaat
     6841 agtcattaat ctctcctttt gtagtggtga acgttcggca atctcctgaa taaagcgggt
     6901 gaaacgggag gtatcgccgc attgcagcag ataattaaac agtgtttgta gctggctgtc
     6961 attagtgaag cctctaacca aaagcgtggt gatcctgtcg accataccga ttaaatcgcg
     7021 gtcgcgaata tgcttttgaa tcagttccag cagagctata cgccgatgtt gcatgatctc
     7081 atcgtcaggt acgatggtga tatccaccaa cggaaaagct tcggtgtaca actgccgggc
     7141 aagttgcgga tcgtcaaact catccagcca gttgagtgag tacgggtagg gcgaggcttc
     7201 gccatgataa aacagcaatg gcaccaccag cggaactctg tcatagcctt tatccaggtg
     7261 acgctgcatg gcggcagtgg catagcgcat tagccgaaaa gccatattct tttctgcaga
     7321 gctttgatgt tcaatcacgc agtagatata gccgtcaccc tcgctggttt ccacggacca
     7381 caaaacatcc gagtagtaag cgcgtaaact tttttcaata aaactggtgg gttccaggcg
     7441 taaggtttgc aggttgcaaa gcttgcgcag cggctctggt agatgtattt ccagaaaatc
     7501 ccgtgcggtt tcgggtgtga acataaaggt tttaaacacc gcatcatgcg gggaggaggt
     7561 tgttgattcg gtcatggttg tccgtcacct gatatttatg atgacgcgac attacggtta
     7621 tcccaggcct ttgacatctg ccagtttcag cctttgcgag acgttttcag aggaattttc
     7681 actgcgttgc aacgcataca ttgggcacgg aatcgtgtac gcaaaaatgc ccggtaacaa
     7741 catgcttacc gggcatcaga aaggagttta agccagcgcc tgtgccagca gcgtaatcgg
     7801 atgttcgcag cgaagacttg tggacatctc aatctgccat ttacaggttt cgcagtcggt
     7861 gaccaccaga tctgcgccgc tctcttctat ctggcggaac agtggtgcgc cgatggcttg
     7921 tgaagtgggg tagttctctt ttttgaaacc gtaagttccc gcaataccgc agcactggga
     7981 atccagcacc gttaactcaa gccccgggat tttacgcaac agctctaggg tgtagagcgt
     8041 ccagcccatt ttttccatat ggcacggagt gtgataaacc actttcagcg gcagcggttt
     8101 cagcggtaac gttttgcctt cgtccagctt gcgccacagc cagcgggtcg ccagttcgat
     8161 atgatcgcgc aagcctttgt tgtcgacatt cagcacttcc gggtattcgt cgcgcagggc
     8221 aaatgtacaa gttgaggagg tggcaatcac cggaatgcct tttactccca cagcttcgcg
     8281 gatcgactct acattcgtaa ttgcctgttt gcgtgcttta tcggtaaaac cgttggcgat
     8341 cagcggtacg ccgcaacatt tttctttgct gagcagttgt acgccggtac ccatcgcgtt
     8401 gagcacttta attaaatctt tgcctagctg cggatggttg tagttaacga agcagccgtg
     8461 aaagaaagcg acctggtctt tatactgcgc ttgctgagcc gccacgctgc gataccagcg
     8521 acggaacgta ccgaaggagt atttcggtag cgtgcgacgg tggtcgattt ttaacgccgc
     8581 atcaagcaac tgccgcaccg gtttcagcga ggtagcagtg ttgacgatgg gggcgaacgg
     8641 cgtggaaacg ctacccatca ggtcggtatg actcaacaca aaattacgca gcgacgggcg
     8701 tgtggtgtca tatttcgccc gcgcgcgctg gataatatcg ccaatcttca catcggacgg
     8761 gcaggcgact tcacaacgtt tgcagttgat gcaatatttc agcgcctcgt catacagtgc
     8821 gccatctttt aaacgcagac gctcgccatc cggcccggct tgttttggcc ccggataacc
     8881 cggattcacc cggctcaccg ggcaggcggt ggtgcagacg gtgcacttaa tgcagttttc
     8941 gaagctggtg tcattcattg ttggcctcct gcgcgttggg caatctgttg agcgacatgt
     9001 aaagcactga cggcacaaac accgccgccg catccctggg cgatgggatc aaatccgccc
     9061 agcaccgaac cgatggcaaa caggttctca atggtttgcc ccgcctgcga cgggcgtagc
     9121 gcctcatcag tggttacacc gaactgctgc cacggctgcg gcgcaaaaaa atcgccctta
     9181 taccattcac cccgcgtagc ggtttgtagc acatcaaggc cgagaatcgg ctcgcgaatg
     9241 ccgtcacgtt ctgccaccag tccgccgcta aagaaactgc cgctcgcaag caccgcaaat
     9301 cgtgggcgca gcgggatatc ggcgtgattg cgcgtccaga tttcattcac tacaccattt
     9361 ttacaggtca cttttttcac ttcatcgccc ggcatccaca cgccaccctg gcgcacaaac
     9421 tggcgctgta actggttttg cagacgaatg cccagcacgg aaggcggcag cgttggcaaa
     9481 agcatcaatg aacaaggtag tttttcattc aaccaatgcc acagtttgtc atcggccagg
     9541 ccgaagcagg cgggcatcag gatcatttcg caggtattgg cgaccggaat aagtgcatca
     9601 agtaacaacg gccagttttc ttcattatca aggaagcggg caatattcac cgcccggaac
     9661 tcggtggcgt tattgcgcag cacatccagt tccggcagtt caatttcagc ggtttcaact
     9721 ttaagatcca attcgcgcaa tgaagccgcc gcaagatgcg cctgaaaatc catcaggccg
     9781 ctaattccca ctacacatat tttcttcgcg ggcagcggcc agacagggac ttctggcgaa
     9841 cttagccagg ttgagcgcag agtgcctagc ggtgtaatcc gctggtgagc cagctctacg
     9901 ctgccctgca attgcgcacc gctttcagcg attaatgcct gcgcctggca agcgagatcg
     9961 agcacgcgtt gtggcccgag aagagtgtaa ggatgggcag gtgcctgctg acgcaaagat
    10021 tccagtccac tgtgaatttc tgtcaccggt tgaccatccg gcagatggct cagcaaatcc
    10081 agcgatccag atgagaaatg cagcgcgctt tgaccacgag tgacaatggc acagcgcagg
    10141 ccgtgttttt gcagttgcag gccacagagt aatccggcga ggccgccgcc cataatgaca
    10201 gtatcaaagc gcatctttct gctccttctc cagaccacac aatccctgat aaacccagcg
    10261 ggtaaattcg ctttcgcgca gtgcatctcc ccaggcgatg ggttgcacgc ctttccagcg
    10321 ttcgtttagg aaggtggaaa gttgctcgat agattgcgcg gacgtcgtga cgttaaaacg
    10381 ttgcagcagt ccggcagcgc ggcaggcgca gagttcgccc tggcaggtgc ccatccccac
    10441 acgggtacga cggcgtaaat ccagcaggct attaacgttt aaattttcta ccgcgtactg
    10501 cacttcaccc gcagttaccg cttcgcactc acataccagg ctacggtgca gacggccttc
    10561 gctcagccag gcaggcgtgc gatcgccatg acgataaacc gcagaaccgc gcagcggggc
    10621 aggcagggag atgactttac gcaaggtagc ttcagcgggt tcctgtgaac caggaagtgc
    10681 cagatcggcg gtcgtacagg ggcgcgtgtt gcccagtttg cggcatacgg cgtcggtagc
    10741 ccattccgcc attagccgat aggtcatcag tttgccaccg gtgatggtga taaacccgtc
    10801 cagaccatcg cgttcagcat ggtcgagcag cacgatgcca cggctgacgt tacgcccgct
    10861 cggatcgtca tcgctggcaa ccagcgggcg tacgccagag taggcccgca aaatgcgcgt
    10921 tttcgccatc acgggggcca gtttttcccc ttcacgcagc agaatatcaa cttcttctgc
    10981 cgtcactcga ttatcgtcaa tctcgttgta atcaatacgt aaagaggtgg tgccaatcag
    11041 cgaaatggta tcgccaggca ccagaatatc ggcgtcggaa ggtttgcggc agcggttgat
    11101 cacatgctga ttaatgcggt gatccatgat cagcagcgat cctttcgccg ggaacatgcg
    11161 aatgcgcaga tcggcatatt cggcaatgtg ttgcccccag atcccggcgg cattaactac
    11221 gacaggcgca tgaagtgcct gaatttcgcc ggtgagatgg ttacgtacac gaacaccgca
    11281 caccgtcgcg ccttcacgaa tcagccccgt gacttcatga gcggtaagga taacggcacc
    11341 gtgttctttg gcatccagca tgtttgctgc tgtcagacga aatggatcaa cggtgccatc
    11401 cggaactttc accgcgccaa tcagtgccgg gttaacggca ggttcgataa tgcgcgcttg
    11461 ctgcggctca atggcttctg cgttgatccc tgcttcttcg caggcgcgga taaaagtggc
    11521 ctggaaggag aggtcatctt ccggcagggt gataaacagg ccgttggttg gttctacgca
    11581 gtgacgtgca atgcgtttca gaatctggtt ttcactaata cattcgcggg ccgattccgc
    11641 atcggttacc gcatagcgcg caccgctgtg cagcaggccg tggttacgcc cggtggcacc
    11701 ggttgcgatg tcgtggcgct caaccaaaat cacgcgcagc ccgcgcaggg cacagtcgcg
    11761 ggcaatcccg gctcccgttg cgccaccgcc aatgataatc acgtcacttg attgcgagtc
    11821 gcgagttttc attgttatcc ctctgaagtt cgttttttac catttagcca tagtaaaaac
    11881 atgaattgtt tgatttcgcg catattcgct cataattcga aagtgaaacg tgatttcatg
    11941 cgtcattatg aacatttcaa ccatcgtatt taataatgtg tgcggtaatt cacatttaat
    12001 ttatgaatgt tttcttaaca tcgcggcaac tcaagaaacg gcaggttctc ccactgaatc
    12061 aggctgttaa tcataaataa gaccacgggc cacggaggct atcaatgttg agtattttta
    12121 aaccagcgcc acacaaagcg cgcttacctg ccgcggagat cgatccgact tatcgtcgat
    12181 tgcgctggca aattttcctg gggatattct ttggctatgc tgcttactat ttggttcgta
    12241 agaactttgc gctcgctatg ccttatctgg ttgagcaggg attctcacgc ggtgatttag
    12301 gttttgccct ttcggggatc tcgattgctt atggattttc gaaattcatc atgggttcgg
    12361 tatcggatcg ctcgaatccg cgcgttttcc tgcccgcagg tttgattctg gcggcggcag
    12421 tgatgttgtt tatgggcttt gtgccatggg cgacgtcgag cattgcggtg atgtttgtac
    12481 tgttgttcct ctgcggttgg ttccagggga tggggtggcc gccgtgtggt cgtactatgg
    12541 tgcactggtg gtcgcagaaa gaacgtggcg gtattgtgtc agtgtggaac tgtgcgcaca
    12601 acgtcggtgg tggtattccg ccgctgctgt tcctgctggg gatggcctgg ttcaatgact
    12661 ggcacgcggc gctctatatg ccagctttct gtgccattct ggtggcactg ttcgcctttg
    12721 cgatgatgcg cgataccccg caatcctgtg gcctgccgcc gatcgaagag tacaaaaatg
    12781 attatccgga cgactataac gaaaaagcgg aacaggagct gaccgcgaag cagatcttca
    12841 tgcagtacgt gctgccgaac aaactgctgt ggtacatcgc cattgccaac gtgttcgttt
    12901 atctgctgcg ttacggcatc ctcgactggt caccgactta tctgaaagag gttaagcatt
    12961 tcgcgctgga taaatcctcc tgggcctact tcctttatga atatgcaggt attccgggca
    13021 ccctgctgtg cggctggatg tcggataaag tctttcgtgg taaccgtggg gcaaccggcg
    13081 tcttctttat gacactggtg accatcgcga ctatcgttta ctggatgaac ccggcaggta
    13141 atccaaccgt cgatatgatt tgtatgattg ttatcggctt cctgatctat ggccctgtga
    13201 tgctgatcgg tctacatgcg ctggaactgg caccgaaaaa agcggcaggt acggcagcgg
    13261 gttttaccgg gctgtttggt tacctcggcg gttcggtggc ggcgagcgcg attgttggct
    13321 acaccgtgga cttcttcggc tgggatggcg gctttatggt aatgattggc ggcagcattc
    13381 tggcggttat cttgttgatt gttgtgatga ttggcgaaaa acgtcgccat gaacaattac
    13441 tgcaaaaacg caacggaggc taatggcatg aaattgacgc tgaaaaacct tagcatggcg
    13501 atcatgatga gcactatagt catgggaagc agtgcaatgg cggcggacag caacgaaaaa
    13561 atagtcatcg cccatcgcgg tgccagtgga tatttgccgg agcatacgct gccagcaaaa
    13621 gcgatggcgt atgcgcaggg agcggattat ctggaacagg atttggtgat gaccaaagac
    13681 gaccatctgg ttgttctgca tgaccattat ctcgatcgtg ttactgatgt tgccgatcgt
    13741 ttcccggatc gggcgcgcaa agacggtcgt tactacgcga tagatttcac gctggatgaa
    13801 attaagtcgc tgaaatttac cgaaggtttc gatattgaaa acggtaaaaa agtgcagact
    13861 tatccggggc gtttcccaat gggtaaatct gacttccggg tgcacacctt tgaagaagag
    13921 attgaatttg ttcaggggtt aaaccactct accgggaaaa atatcggtat ctatccagaa
    13981 attaaagcgc cgtggttcca tcatcaggaa gggaaggata ttgcggcaaa aacgctggaa
    14041 gtgctgaaga aatatggtta caccggtaag gacgacaaag tttatttgca atgttttgat
    14101 gctgatgagc tgaagcgtat taagaatgag ctggaaccta aaatgggcat ggatctcaat
    14161 ctggtacagc tgattgccta taccgactgg aatgaaacgc agcagaaaca gccggacgga
    14221 agctgggtta attacaatta cgactggatg tttaagccgg gtgccatgaa acaggtggcg
    14281 gaatatgctg atggtattgg tccggattac catatgttga ttgaggagac atcgcagccg
    14341 ggtaatatca aactcactgg catggtgcaa gatgctcagc agaataaact ggtagtgcat
    14401 ccttataccg tgcggtcaga taaactgcct gaatacacta ctgatgtgaa tcagttatat
    14461 gatgctctgt ataacaaagc gggtgtaaat gggctattta ctgatttccc tgataaggca
    14521 gtaaaattcc ttaataaaga gtaaattaac taccggatgg cacttatgtg ctatccgata
    14581 ccactcttca ttaaagtctg tgggttatcc ttaattgcaa ttatttagag cgagatgact
    14641 tgttcccggc ataagatact cgtcaataca acacgtatta ttaagatttt cctcagataa
    14701 acatctatag tcattgtcgt taataaaaaa cgacaggcga tagggtatgg cagtttcagc
    14761 aaagcacgac gaatttaatc actggtgggc aacagagggc gactgggttg aagagcctaa
    14821 ctatcgccgt aatggtatga gcggggtgca atgcgtcgag cgcaacggca aaaagctgta
    14881 tgtaaagcgc atgacgcatc atctgtttca ttccgtacgt tatccgttcg gccgaccaac
    14941 gattgtccgt gaggttgcgg taattaaaga actggaacgg gccggtgtca tcgtgccgaa
    15001 gatcgttttt ggtgaagcgg tgaaaattga gggtgaatgg cgcgcgttgc tggtgactga
    15061 agatatggcg gggttcatca gcattgctga ctggtatgcc cggcatgcag tatcgcctta
    15121 ttctgacgaa gtacggcaag ccatgttgaa agcagtggcg ctggcgttta agaaaatgca
    15181 tagcgttaat cgtcagcatg gctgttgtta tgttcgccat atctatgtga aaacagaagg
    15241 taaggcagaa gctggttttc ttgatctgga gaaaagtcgt cgccgcttgc gtcgtgataa
    15301 agcgattaat catgacttcc gccagctgga aaaatatctg gagccgatcc ccaaagcgga
    15361 ctgggagcag gtgaaagcat attactacgc tatgtaatcg ttgtaggcca gataagacgt
    15421 ataagcgtcg tatctggcgt taaaaccaat tcacatctcg atttcaatat cgccttttgc
    15481 ccggcaacaa cagggcaaaa tttcccccgg ctgaataaaa gctaatggtt cggcaatcca
    15541 gtccacctgg cctgcgacca gtcgggtgcg acaggagccg cagtaacctt cgcggcactg
    15601 gtactcaacc gccacattgt gggattccag cgccgccaga agggaagggt gttcatcctg
    15661 gcacagcagt tgtgtgccag taatgcgcag ggtaacgcgg gccatcagag ctggaagtta
    15721 ctcaaatcgt cggtgtccac ttccgcgtca atctgcccga ccagataaga actgacttcc
    15781 acttcctgcg gagcaacctg cacgttatca gacaccagcc aggtgttgat ccacgggatc
    15841 gggttggagc gcgtctggaa cggcagatcc aaaccgactg cctgcatacg gatattggtg
    15901 atgtattcaa cgtactggca gaggatgtct ttattcagac caatcatcga accatcacgg
    15961 aacagataat ccgcccagtc tttctcttgc tgtgccgcct ggacaaacag gtcatagcac
    16021 tcctgcttac attcttcggc aatttccgcc atctccggat catccgcgcc gctgcgcagc
    16081 agattcagca tatgctgggt gccggtcagg tgcagggctt cgtcgcgggc aatcaggcga
    16141 ataattttgg cgttaccttc catcagctca cgttcagcga atgcgaaaga gcaggcaaag
    16201 ctgacgtaga aacggatcgc ttccagcgcg ttaacgctca tcaggcagag atacagtttt
    16261 ttcttcagct cgcgcaggct aacggttacg gttttaccgt taacggtgtg ggtgccttcg
    16321 cccagcagat gccagtagct ggtcatttcg atcagttcat cgtagtagct ggagatccct
    16381 tccgcacgtt tctggatctg ctcgttagtg acgatatcgt caaacacaac agacggatcg
    16441 ttaacgatat tacggatgat atgggtatag gagcgggaat gaatcgtttc tgagaacgcc
    16501 caggtttcta cccaggtttc cagttccggg atcgagatca gcggcagcag tgccacgttc
    16561 ggactgcgac cctgaatgga atccagcagc gtctgatatt tcaggttact gataaagatg
    16621 tgtttttcgt gttcaggcag tgcctggtaa tcgatacggt cgcgggagac gtcaacttct
    16681 tccggacgcc agaagaaaga gagctgcttt tcgatcagct tttcgaagat gtcatatttt
    16741 tgctgatcgt agcgagccac gttgaccggc tgaccaaaga acattggttc tttgagctga
    16801 tcatttttcg tctgtgaaaa ggtggtatat gccatgaatg tgtcctgtgg ttttattacc
    16861 cctcacccgt cccctctccc gcaaggggag aggggacggt ttggtgcaaa ggctggggga
    16921 atatcagatc ttacatgcgc cgctttcgca gccatcgtcc tggattgacg gcaccagatc
    16981 gtcctgcgcg tcttctgcgc cgtcacgggt gttctgataa tacagcgttt tgaccccgaa
    17041 tttgtaggca gtgagcaggt ctttcagcaa ctgctgcatc ggcacttttc ctgacgggaa
    17101 acgtgacgga tcgtagttgg tgttagcaga gatcgactga tcgataaatt tctgcatgat
    17161 acccaccagt tgcaggtagc catcgttacc cggcatttcc cacagcagct cataggcgtc
    17221 gtgcagatgc tcgtagtccg gcaccacctg gcgcagaata ccgtctttcg acgctttgat
    17281 gctgacgtaa ccgcgcggcg gttcaatacc gttagtggcg ttagagatct gcgaagaagt
    17341 ctcggacggc atcagggcag aaagcgtgga gttacgcagg ccgtgcgttt tgattgactc
    17401 acgcagtgct tcccagtcgt aatgcagcgg ctcattagcg atggcatcca gatctttctt
    17461 ataggtatcg atcggcagga tccctttcgc gtaagtggtt tcgttaaacc acgggcacgc
    17521 gccttgctct ttcgccagct cattagacgc tttcagcaga taatactgaa tggcttcgaa
    17581 ggttttatgc gtcaggttgt tggcgctgcc gtcggagtag cgtttaccgt gcttcgccag
    17641 gtagtaagcg aagttgatca caccaatacc cagcgtacga cgacccatcg ctccacgttt
    17701 ggcggccggg atcgggtaat cctgataatc cagcagcgcg tcgagtgcgc gtactgccag
    17761 aattgccagc tcttccagtt catccaggct gttaattgcg cccaggttga aagcagacag
    17821 cgtacacagc gcgatttcac cgttctcgtc gttgacgtcg ttcagcggtt tggtcggcag
    17881 ggcgatttcc aggcacaggt tagactggcg cactggcgcg atggccggat caaacgggct
    17941 atgggtatta cagtggtcaa cgttctgaat atagatacga ccggtagatg cacgttcctg
    18001 catcatcagc gagaacagct caacggcttt cacacgctgc ttgcggatgc tgtcgtcttt
    18061 ctcatatttg gtatacagac gttcaaactc ttcctgatcg gcgaagaacg cgtcgtacag
    18121 ccccggtacg tcggacgggc tgaacagagt gatatcttca cctttcagca gacgggtata
    18181 catcagtttg ttgatttgaa ccccgtagtc catatgacgc acgcggttgc cttccacacc
    18241 acggttgttt ttcaacacca gcaggctttc cacttccaga tgccacatcg ggtagaacag
    18301 cgttgccgca ccgccgcgca caccgccctg agagcaggat ttcaccgctg tctggaaatg
    18361 tttgtagaac ggaatacaac cggtatggaa cgcttcgcca ccgcgaatcg ggctacccag
    18421 cgcacgaata cgcccggcgt tgatgccgat cccggcacgc tgggaaacgt atttaacaat
    18481 cgcgctggag gtggcgttaa tggaatccag gctgtcgccg cactcgatca gcacgcagga
    18541 gctgaactga cgagtcgggg tacgcacgcc ggacatgatt ggcgtcggca gcgaaatttt
    18601 aaatgtggaa accgcgtcgt aaaaacgctt cacgtattgc aggcgcgttt cacgcgggta
    18661 gttcgagaac aggcacgcgg caaccagaat ataaaggaac tgggcgcttt cgtagatttc
    18721 gccggtcacg cggttctgta ccagatattt gccttccagc tgcttaacgg cagcataaga
    18781 gaaggtcata tcacggtcgt gatcgataaa cgtgtccatc tgcttgaact cttcttccgt
    18841 gtagtcttcc agcagatgat tatcgtattt gcccatctcg accattttca ccacatggtc
    18901 gtacagcgca ggcggctcaa actgaccgta ggctttttta cgcaggtgga agatcgccag
    18961 acgtgcggcg agatactgat aatccggcgc atcacgggag atcaggtctg cggcagcctt
    19021 gataatagtt tcgtggatgt cagaggtctt gataccgtca taaaactgaa tgtgggagcg
    19081 cagctcgacc tgggaaatcg aaacgttatg cagtccttct gccgcccaat ccagaacgcg
    19141 atggattttg tcgagattga tgcgctctgt gctaccgtcg cgctttgtca ccagcagatt
    19201 ctgattcatg tatgtcgtac ctgtttttca atagcttcat gcagactacc tgtagtgagg
    19261 gaagctgcta agaacactat atataggggg tatgtttggg attcactgca agatagtgtg
    19321 aaactggccc tcttgcaagt gcataacttt gtggataact caggaaggaa aaagtggctt
    19381 ccgcgcacct taggtcagac aaggtgtccg ggaaagtcaa tgggaagaga aaaatttgtt
    19441 aaaaataacc gttcggtgaa ttcttgaacg gtaaaatagg ataggtaaat tgatatagat
    19501 ggtttattaa tcttgttgct aatgattgcc aaaattgatc taggcacaag tcgtcacata
    19561 atctgatgta tcagttgtgg tcaatatata agcgagtgta agcgattgtt gagtcggaaa
    19621 tggttgtttt atattcggtt attgttaaat actaagccaa tttgttactt ttgtatttat
    19681 tataagaata ttat
//
LOCUS       DAZFJL010000088        19203 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFJL010000088 DAZFJL010000000
VERSION     DAZFJL010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.088"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            173..1333
                     /locus_tag="ACHJ8C_003072"
     CDS             173..1333
                     /locus_tag="ACHJ8C_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO5552885.1"
                     /translation="MSIKLRGGTWHCDFVAPDGSRVRRSLKISDKRQAQEFHDRLKAE
                     AWRVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWMLHFRETILSNITTEK
                     IMEAVDGMENRRHRLNWEMSRDRCLRLGKPVPEYKPKLASKGTKTRHLAILRAILNIA
                     VEWGWLDRAPKISTPRVKNGRIRWLTEEESKHLFAEIAPHFFPVVMFAITTGLRRSNV
                     TDLEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQGLHKRWVFVHTRPA
                     YRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWASWLVQSGVSLLALK
                     EMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNTAQEVNVVYLNVR"
     gene            complement(1348..1423)
                     /locus_tag="ACHJ8C_003073"
     tRNA            complement(1348..1423)
                     /locus_tag="ACHJ8C_003073"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1388..1390),aa:Thr,seq:cgt)
     gene            complement(1538..2791)
                     /gene="proA"
                     /locus_tag="ACHJ8C_003074"
     CDS             complement(1538..2791)
                     /gene="proA"
                     /locus_tag="ACHJ8C_003074"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO5552886.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSESILN
                     ANAQDVADACANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(2803..3906)
                     /gene="proB"
                     /locus_tag="ACHJ8C_003075"
     CDS             complement(2803..3906)
                     /gene="proB"
                     /locus_tag="ACHJ8C_003075"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO5552887.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            4195..5250
                     /gene="phoE"
                     /locus_tag="ACHJ8C_003076"
     CDS             4195..5250
                     /gene="phoE"
                     /locus_tag="ACHJ8C_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO5552888.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(5289..5690)
                     /gene="crl"
                     /locus_tag="ACHJ8C_003077"
     CDS             complement(5289..5690)
                     /gene="crl"
                     /locus_tag="ACHJ8C_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO5552889.1"
                     /translation="MTLPSGHPKSRLVKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(5748..6992)
                     /gene="frsA"
                     /locus_tag="ACHJ8C_003078"
     CDS             complement(5748..6992)
                     /gene="frsA"
                     /locus_tag="ACHJ8C_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO5552890.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(7084..7542)
                     /gene="gpt"
                     /locus_tag="ACHJ8C_003079"
     CDS             complement(7084..7542)
                     /gene="gpt"
                     /locus_tag="ACHJ8C_003079"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO5552891.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            7803..9260
                     /gene="pepD"
                     /locus_tag="ACHJ8C_003080"
     CDS             7803..9260
                     /gene="pepD"
                     /locus_tag="ACHJ8C_003080"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO5552892.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKAHGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(9317..9931)
                     /gene="prfH"
                     /locus_tag="ACHJ8C_003081"
     CDS             complement(9317..9931)
                     /gene="prfH"
                     /locus_tag="ACHJ8C_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO5552893.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(9928..11067)
                     /locus_tag="ACHJ8C_003082"
     CDS             complement(9928..11067)
                     /locus_tag="ACHJ8C_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO5552894.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAALFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRNSLGSIGGGNHFAELQQIDQIIDAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARINRQL
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(11258..11710)
                     /locus_tag="ACHJ8C_003083"
     CDS             complement(11258..11710)
                     /locus_tag="ACHJ8C_003083"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5552895.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITCIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            complement(11707..12762)
                     /gene="dinB"
                     /locus_tag="ACHJ8C_003084"
     CDS             complement(11707..12762)
                     /gene="dinB"
                     /locus_tag="ACHJ8C_003084"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO5552896.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVTLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKSDLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(12833..13756)
                     /gene="lafU"
                     /locus_tag="ACHJ8C_003085"
     CDS             complement(12833..13756)
                     /gene="lafU"
                     /locus_tag="ACHJ8C_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO5552897.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPSKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPENKVMQVSAMADQMLLDAKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRAR"
     gene            complement(13759..14622)
                     /gene="motA"
                     /locus_tag="ACHJ8C_003086"
     CDS             complement(13759..14622)
                     /gene="motA"
                     /locus_tag="ACHJ8C_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO5552898.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(14635..15351)
                     /locus_tag="ACHJ8C_003087"
     CDS             complement(14635..15351)
                     /locus_tag="ACHJ8C_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HFO5552899.1"
                     /translation="MLQDVFESEEFAATLVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWQELAEEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLAGRELEDQFVTQQMLQHALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(15371..15838)
                     /locus_tag="ACHJ8C_003088"
     CDS             complement(15371..15838)
                     /locus_tag="ACHJ8C_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HFO5552900.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWSVWHHYAGKGKPTTAAQTES
                     VEALDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            complement(15846..16907)
                     /locus_tag="ACHJ8C_003089"
     CDS             complement(15846..16907)
                     /locus_tag="ACHJ8C_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO5552901.1"
                     /translation="MNPALLATLGTLAETASLKADILPPVSSENAPAFTLPKMAVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQVTPQPRNVATSPVIQQL
                     TKAVVQNAAQRPTQQQELTPLPPQLQELISQLPQAKPEQQARLAIYASEDLHAIAPTQ
                     PRVSTQPARPKPELTRVTARPQVERKTEKVSDSEPVIARAVLQVKTPELVSDHQEIVA
                     KPATLSIDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(16904..17215)
                     /locus_tag="ACHJ8C_003090"
     CDS             complement(16904..17215)
                     /locus_tag="ACHJ8C_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HFO5552902.1"
                     /translation="MEKQRRQLFALVEAMNEALDKQRWRRLPALHQQLMRQFHDYAAA
                     ETDAAQLDTVKAQLYGAFNTLIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(17220..17612)
                     /gene="fliS"
                     /locus_tag="ACHJ8C_003091"
     CDS             complement(17220..17612)
                     /gene="fliS"
                     /locus_tag="ACHJ8C_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO5552903.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAR
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(17635..18951)
                     /gene="fliD"
                     /locus_tag="ACHJ8C_003092"
     CDS             complement(17635..18951)
                     /gene="fliD"
                     /locus_tag="ACHJ8C_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO5552904.1"
                     /translation="MINPRTIAQEIAYADVATQAANLQEKQTELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSATVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSVADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDTSNDSTSAPVATDVSSAQDAIIHLGS
                     ATGPEITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSTSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDEMAKNPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(19018..>19203)
                     /locus_tag="ACHJ8C_003093"
     CDS             complement(19018..>19203)
                     /locus_tag="ACHJ8C_003093"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5552905.1"
                     /translation="WTRRPDKTRQRRIRHCAPIAGCGVNALSGHEQHRLWAVLCFLRQ
                     AFFRQTGNVISACFSIY"
BASE COUNT         4553 a         5146 c         4810 g         4694 t
ORIGIN      
        1 acaaagacac tatcacgcag tggatacgaa gtggacgact tcaggccgca aagataaatc
       61 cagataaacc taaaagccca tatcgcattt gcaagtcaga ctgcattgcg gcgcttaagt
      121 ctgtaagaca caatagcgcg gtgaatgcgg ttgatgtgca ggaggttaaa gcatgtcaat
      181 caaactacgc ggtggcacgt ggcactgcga tttcgtcgcg ccagatggat caagagttag
      241 acgctcactt aaaatatcgg acaaaaggca agcgcaagaa tttcacgatc gtctgaaagc
      301 agaagcgtgg agagtaaaaa atctcgggga atcaccgaaa aagctattca aggaagcctg
      361 catacggtgg ctgcgtgaga aatcggataa gaagtccatt gatgatgaca agagcattat
      421 atcgttctgg atgttgcact tcagagaaac cattctctct aacataacaa cagaaaaaat
      481 aatggaggcg gtagacggga tggaaaaccg ccgccatcgc ctgaactggg agatgagccg
      541 ggacaggtgt ttgcggcttg gcaagccagt gccggagtat aaaccaaagc tggcaagcaa
      601 aggaacgaag acgcggcatc tggcaatact tcgcgctatt ctcaatatag ctgttgaatg
      661 gggatggctt gacagggcgc ccaaaatatc aacaccacgc gttaagaatg gacgaatcag
      721 atggcttaca gaggaggaat cgaagcacct gtttgcagaa attgctcctc atttcttccc
      781 tgtggtcatg tttgcaatca cgacaggcct tcgccgttcc aacgttacag accttgagtg
      841 gtcacaggtc gatctggata agaaaatggc atggatgcac cctgatgaaa caaaagctgg
      901 caatgcgatc ggagttcctc ttaacgaaac cgcatgccag atattaagaa aacagcaggg
      961 gctccataag agatgggtat ttgtccacac cagacctgcc taccgaagcg acggaacaaa
     1021 aacagcagcg gtaaggaaga tgagaaccga cagcaacaag gcatggaagg gagcgttaaa
     1081 gcgggcgggc attagcaact tccgcttcca tgacctgagg catacctggg caagctggct
     1141 ggttcagtcc ggtgtctctc ttcttgcact taaagagatg ggaggatggg aaactctcga
     1201 aatggttcaa agatacgccc acctttcagc cgggcatctc accgagcacg cgagcaaaat
     1261 cgatgcgatt ataagtcgca atggcacaaa tacggcacaa gaggtgaacg tggtttactt
     1321 aaatgtgagg taatttattg atttaaatgg tgccgataat aggagtcgaa cctacgacct
     1381 tcgcattacg aatgcgctgc tctaccaact gagctatatc ggccctgaaa ggacatgttc
     1441 acgaacgtga atcacggtgg acaaggttaa aactaaccgg gcgatgcgtc aatggccttg
     1501 tgaatcaaat ggctactttt gcatcacccg gttttattta cgcacgaatg gtgtaatcac
     1561 caatgccgat ccacttgtaa gtggtcagtg cttccagccc cattgggccg cgcgcgtgga
     1621 gtttttgtgt gcttaccgcc acttccgccc ccagtccaaa ctggccgccg tcggtaaaac
     1681 gcgtagaggc gttaacgtaa acagcggagg aatccacttc gttaacaaaa cgctgggcgt
     1741 tgcgcatatc gcgggtcagg atcgcatcgg agtgttgcgt gccgtgttca cgaatatggg
     1801 caatggcgtc atccagatcg ctaacgattt tgacgttcaa atctaatgac agaaactcat
     1861 cgtcatactc ttcggcttta acagccacca ccttcgcggg gcctgcctgc aactgcgcca
     1921 gcgcggctgc atcagcgtgt aacgtcacgc cgctttccgc catttgtttg cttaatgcgg
     1981 gcaggaagct atcagcgatg tttttattca ccagcaacgt ttctaccgta ttacatgtgc
     2041 tcggacgctg agttttcgcg ttgacaatta ctttcagggc ttcagcgatc tctgcacttt
     2101 catcaacgta aatatggcat acgcctatac cgcctgtgat caccgggatt gtcgactgct
     2161 cgcggcacag tttatgcaaa ccagccccac cgcgcgggat cagcatgtcg atgtatttat
     2221 ccatacgcag catttcactg accagcgcac ggtcaggatt atcaattgcc tgcacggcac
     2281 ccgccggtaa gccgcaggat ttcagggcgt cctgaatcac cgctaccgtt gcagcgttag
     2341 tgcgacaggt ttctttgccg ccgcgcagga tcaccgcgtt accggttttc aggcacaggg
     2401 aagcaacatc aaccgtcacg ttcgggcgcg cttcataaat cacgccaatc acccccagcg
     2461 gtacgcgacg acgctcaaga cgcaggccgc tgtccagtac gccgccatcg attacctgcc
     2521 ccaccggatc ggcgagattg cacacctggc gcacatcgtc ggcaatgcct ttcagccgtg
     2581 cgggcgtcag tgccagacgg tcaagcatcg cttcgctaag gccattggca cacgcgtctg
     2641 caacatcctg cgcgttagcg ttgaggatac tttcgctttg tgcttccagt tcatcggcga
     2701 ttttttccag cacacgattt ttttcgcggc tggagagttg cgctaattta tacgaggctt
     2761 gcttcgcggc aatgcccatt tgttccagca tcggccagct ccttaacggg taatcatgtc
     2821 atcacggtga acggcaaccg ggccgtattc atatcccagt attgcatcaa tttcttgcga
     2881 gtggtgtccg gcaatacggc gtaatgcatc gctgttgtaa cgactgacgc cgtgggcgat
     2941 atcgcgacct tcgaggttgc aaatacggat gacttcacca cgcgagaaat tgccagtcac
     3001 gcttttaatg cctttcggca acagggagct gccgcgttcc agaatggcgg cagttgcccc
     3061 ttcatctacc gtgatttcac ccgcaggcgg cgcaccgaaa atccagcgtt tacggttttc
     3121 aagcggagtc gcctgggcat ggaacagcgt accgacggaa atgccttcca tcacatcacc
     3181 aataacgccc ggcttgctgc ccgcggcaat aatggtgtcg atacccgcac ggcaagccac
     3241 gtcagcggcc tgcaatttgg tgctcatacc gccagttccg aggcctgaaa cgctatcacc
     3301 ggcaatcgcg cgcagtgcgt catcaatgcc gtacacatct ttaatcagtt ctgcctgcgg
     3361 attgctgcgc gggtcagcgg tatacaaacc tttttgatcg gtcagcagca acagtttatc
     3421 ggcacccgcc agaatcgccg ccagcgcaga aaggttatcg ttatcaccga ccttaatctc
     3481 tgcagtagcg acagcatcgt tctcattaat taccggaacg atattgttat cgagcaacgc
     3541 acgcagggta tcgcgggcgt tcaggaagcg ttcacgatct tccatatcag cacgggtcag
     3601 caacatctgc ccgacatgaa tgccgtaaat tgaaaacagc tgttcccaca gttgaatcag
     3661 tcgactctgc cctaccgccg ccagcagctg ttttgaagcg atagtcgctg gcaattccgg
     3721 gtaccccagg tgctcacgtc cggcggcaat cgcacctgac gtcacaataa caatccgatg
     3781 cccggcggca tgtaactgcg cgcactggcg aacaagttca acgatatggg cacggttcag
     3841 gcggcgcgat ccgcctgtta gcacactggt gccgagtttt accaccagcg tctggctgtc
     3901 actcatgatt ctctgccatt caattttagg aaaaaatgat atcaaacgaa cgttttagca
     3961 ggactgtcgt cggttgccaa ccatctgcga gcaaagcatg gcgttttgtt gcgcgggatc
     4021 agcaagcgta gcggcagttg tttacgcttt tattacagat ttaataaatt accacatttt
     4081 aagaatatta ttaatctgta atatatcttt aacaatctca ggttaaaaac tttcctgttt
     4141 tcaacgggac tctcccgctg aatattcgcg cgttaattaa aatcaggaat gaaaatgaaa
     4201 aagagcactc tggcattagt ggtgatgggc attgtggcat ctgtatccgt acaggccgca
     4261 gaaatatata acaaagacgg taataaactg gatgtctatg gcaaagttaa agccatgcat
     4321 tatatgagtg ataatgacag taaagatggc gaccagagtt atatccgttt tggttttaaa
     4381 ggcgaaacac aaattaacga tcaactgact ggttatggtc gttgggaagc tgagtttgcc
     4441 ggtaataaag cggagagtga tactgcacag caaaaaacgc gtctcgcttt tgccgggttg
     4501 aaatataaag atttgggttc tttcgattat ggtcgtaacc tgggcgcgtt gtatgacgtg
     4561 gaagcctgga ccgatatgtt cccggaattt ggtggcgact cctcggcgca gaccgacaac
     4621 tttatgacca aacgcgccag cggtctggcg acgtatcgga acaccgactt cttcggcgtt
     4681 atcgatggcc tgaacttaac cctgcaatat caagggaaaa acgaaaaccg cgacgttaaa
     4741 aagcaaaacg gcgatggctt cggcacgtca ttgacatatg actttggtgg cagcgatttc
     4801 gccatcagtg gagcctatac caactctgat cgcaccaacg agcagaacct gcaaagccgt
     4861 ggcacaggca agcgtgcaga agcatgggca acaggtctga aatacgatgc caataatatt
     4921 tatctggcaa ctttctattc tgaaacacgt aaaatgacgc caatctctgg cggctttgcc
     4981 aataagacgc agaactttga agcggtcgct caataccagt ttgactttgg tctgcgtcca
     5041 tcgctgggtt atgtcttatc gaaagggaaa gatattgagg gcatcggtga tgaagatctg
     5101 gtcaattatg tcgatgttgg tgctacgtat tatttcaaca aaaatatgtc agcgtttgtt
     5161 gattataaaa tcaaccaact ggatagcgat aacaaattga atattaataa tgatgatatt
     5221 gtcgcggttg gcatgaccta tcagttttaa tggatattgc cggatgtgat gcatccggca
     5281 catttcattc acgccgttaa cttcaccggt tcatcacgaa aatcatctgc cggttccagc
     5341 ttcagattca gcgtcgccag cagctcacgc agcttctcgt gaaattcacg cagggtgtgc
     5401 tccaatcgtt caaccacttc ggcatctttt atctgaacac tcgtccagtc gcctgcttta
     5461 tcgaacagac caaactggta actgtaggta aaacgggatt cctgcgcttc cagttccatc
     5521 caccagcccc agaactcacg cacttccggt gccggtttca cgttgacgca tacagccaga
     5581 caatcgaaaa agaatcgatt atcttcgcac ttaccttcac gaatatacgg gcctagtgcg
     5641 gtaaattttt tgactaatct gctcttcggg tgtccactcg gtaacgtcat tgcaatctcc
     5701 tgttgtgatg caactgtttt accaaattgg taaaatttag caaattttta acacaaacgt
     5761 ttttcgatcc agtctgtgat ttcctgaagg cctttgtcaa aattccgata caccgggtta
     5821 aatgggatct ctaataattt accgtcagca gatgacgacg tgattaagcg tgagtcctct
     5881 tccgggctga acggatcgtt cttccagtag cctgataaca ttggcgttgg gcagcgacgt
     5941 cccagcaatc cttgcacttt taatgaatag cgattcaact ccacgcgcaa cgcttcatcg
     6001 gaagcatcat gcatccctaa acgactggcc agaacgtcaa gatacatttc cggcacctgt
     6061 tgctggcact taaaatcact caacagggta tgaactaccg gaccaagaca agcaaccgct
     6121 ttcagacgtg gcgattcaag gtatgccaga cgcacggcaa cgttagcgcc gaaacggaaa
     6181 ccaaaggccg cgacgcgagt gtgatccacc cacggtacgt taggcagcgc ctttaaaacg
     6241 tgctgatgca acaggctgga gtcctgggtg agcttccatt ttgaagaaaa gcccaccgac
     6301 ggcatatcaa tagtcagcat agcaatgccg cgcggcgcaa aataacgttc atacaggctg
     6361 taatagtccg tctgcatcgc atccagacca ccacacatta atactgtcgg gaacgggcca
     6421 tcgcctttcg gcatatgcaa aaagccggtg atgggcgcac cgccgggtac ggtaaactcc
     6481 atctgccgca tcgtgcccgg cagacgctga gcggcctctt cataggcgcg gtttgacaaa
     6541 gcctgcgctt gctcggccag gtcatctcct ttcagatgag gataggcagc aatgttgtac
     6601 aacgtagccg catgcagcca gtgtcgacca ctgagttgcg gatcttcttc cgcacaggct
     6661 ttttgttgcc acaccatcgc ctgggtagcc cactcataaa tccagttgcc gccgcggtag
     6721 ccaatcaccg tatcgtataa atcatcgtcg gtacgttcgg catcgctcat cacaatacgc
     6781 gcctggacgt cgaggatttc acgggggtca atgccgcgcc agatccacat cagacggtta
     6841 atcatgcgat accagtgagg gatggtttta ccatcaagag ccgactgcac aggcagttgt
     6901 gcgccgtgac taaagcggcg gactagcgtc gaggtttcag gatgtttaaa gcggggtttg
     6961 aacagggttt cgctcaggtt tgcctgtgtc atggatgcag cctccagaat acttactgga
     7021 aactattgta acccgcctga agttaaaaag aacaacgccc ggcagtgcca ggcgttgaaa
     7081 agattagcga ccggagattg gcgggacgaa tacgacgccc atatcccacg gctgttcgat
     7141 ccaagtattt tgcgggatat caacaacata atcatcaacc agcggacgac cagccggttt
     7201 tgcgaagatg gtgacaaagt gcgcttttgg atacatttca cgaatcgcaa ccgcagtacc
     7261 accggtatct accaggtcat caataacgat gaagccttcg ccatcgcctt ctgcgcgttt
     7321 cagcacttta agctcgcgct ggttgtcgtg atcgtagctg gaaatacaaa cggtatcgac
     7381 atgacgaata cccagttcac gcgccagtaa cgcacccggt accagaccgc cacggcttac
     7441 ggcaataatg cctttccatt gttcagaagg catcagtcgg cttgcgagtt tacgtgcatg
     7501 gatctgcaac atgtcccagg tgacgatgta tttttcgctc atgtgaagtg tcccagcctg
     7561 tttatctacg gcttaaaaag tgttcgaggg gaaaataggt tgcgcgagat tatagagatc
     7621 tggcgcacta aaaaccagta tttcacatga gtccgcgtct ttttacgcac tgcctctccc
     7681 tgacgcggga taaagtggta ttctcaaaca tatctcgcaa gcctgtcttg tgttgacaac
     7741 attttctgct aaccctgtga cctgcaatac tgttttgcgg gtgatcgaca aggagactta
     7801 acgtgtctga actgtctcaa ttatctccac agccgctgtg ggatattttt gccaaaatct
     7861 gttctattcc tcacccgtcc tatcatgaag agcaactcgc tgaatacatt gttggttggg
     7921 caaaagagaa aggtttccat gtcgaacgcg atcaggtagg taatatcctg attcgtaaac
     7981 ctgctaccgc aggtatggaa aatcgtaaac cggtcgtctt gcaggcccac ctcgatatgg
     8041 tgccgcaaaa aaataacgac accgtgcatg acttcaccaa agatcctatc cagccttata
     8101 ttgatggcga atgggttaaa gcacacggca ccacgctggg tgcggataac ggcattggta
     8161 tggcctctgc actggcggtt ctggctgacg aaaacgtggt tcacggcccg ctggaagtgc
     8221 tgctgaccat gaccgaagaa gccggtatgg acggtgcttt tggcttacag agcaactggt
     8281 tgcaggctga tattctgatt aacaccgact ccgaagaaga aggtgaaatc tacatgggtt
     8341 gtgcgggagg tatcgacttc acctccaacc tgcatttaga tcgtgaagcg gttccagctg
     8401 gttttgaaac cttcaagtta accttaaaag gtctgaaagg cggtcactcc ggcggtgaaa
     8461 tccacgttgg cctgggtaat gccaacaaac tgttggtgcg cttcctggcg ggtcatgcag
     8521 aagaactgga cctgcgtctt attgatttca acggcggcac actgcgtaac gccatcccgc
     8581 gtgaagcctt tgcgaccatt gctgtcgcag ctgataaagt cgacgccctg aaatctctgg
     8641 tggataccta tcaggagatc ctaaaaaacg aactggcaga gaaagagaaa aatctggcat
     8701 tgctgctgga ctctgttgcg aacgataaag ctgccctgat tgcgaaatct cgcgatacct
     8761 ttattcgtct gctgaacgcc accccgaacg gtgtgatccg caactccgac gtggcaaaag
     8821 gtgtggtcga aacctccctg aacgtcggtg tggtgaccat gaccgacaat aacgtagaaa
     8881 ttcactgcct gatccgttct ctgatcgaca gcggtaaaga ctacgtagtg agcatgctgg
     8941 attcactggg taaactggca ggtgcgaaaa ccgaagcaaa aggcgcatat cctggctggc
     9001 agccggacgc taactctccg gtaatgcatc tggtacgtga aacctatcag cgtctgttca
     9061 acaaaacgcc gaacatccag attatccacg cgggcctcga atgtggtctg ttcaaaaaac
     9121 cgtatccgga aatggacatg gtgtctatcg ggccaaccat caccggtcca cactccccgg
     9181 atgagcaagt tcatatcgaa agcgttggtc attactggac gcttctgact gaactgctga
     9241 aagaaattcc ggcgaagtaa ttatttgatt tgctgccgga tggcgtttaa tcgccttccg
     9301 gcagtttcat ccttcattac ccttcgataa aggccatccc tgtaaacgtt cgtcgcgggt
     9361 tgccacgttc aatctggtga tggaacattc gccgctgcga tttcagcgcc gcgctatttt
     9421 cctgttgctg ttgttccagc ttccaggcaa tcagtaatcg cgccagccgt ttgttagcat
     9481 gctgactgcg ctctgactga accttcacgc taataccgga tgccagatgc gtggcgcgta
     9541 ccgccgagtc ggttttattg acatgttgac cgcccggccc tgacgaacgc agcgtctcat
     9601 aacggattgc atccgattgt tcctgctcat cagcggtaaa acgcccaatg cccagaaacc
     9661 agtttttgcg cccatgatga ggccgatacg gactcggaca aatccactgg atagtgccgc
     9721 accagctttc gcttagagcc catgcgttat cgccatccag agaaatcagc gccgaacgca
     9781 gtgtgtcaga gtagcgaccc gtttctgttt ccagcaccgt taccgcgacg tcctgtcggg
     9841 tagcttcttt aatcagcctg tccagtgctt ttttcactgc gagacaacat tcttccggcc
     9901 cctgagcaga ggagagttgt agcaagatca tccttttttc cctccactgt ttttgagcgt
     9961 cagcaccggg cgcagtcgcg cgacaggaat aattaaccca gccagcacca gacattgcac
    10021 cacgctttca gccgatttat aggcttgtgg cgcttcttca aagatgagtt gtttatcgcg
    10081 acaaattacc cgactgccaa gttcagtccg cgagagttgc gtcgccgtgt atttcgctgc
    10141 cagacgccct ttacactcgg tgcgccccca tttacgcccc gccccatgcg ccagcgagtg
    10201 gagcgttttt tcgttcgcaa caggtttaac cagccaggag taatcgccgc gtgaaccggg
    10261 aataatcacc agaccgttgt catccggcgt ggcgccttta cgatgcaacc agccctgttg
    10321 atcgccgatt tggcacgcgc taacaaagtt atgcgccaca tccagaaccg gacttccggt
    10381 ggccttaact tgttgcatta tgcgcaaagc gatcagctgg cgattaatac gcgcaaacgc
    10441 cagcgcatcg tcatgttccg caatataccg tagcgcgtcg tcactgcctt caggcaaacc
    10501 atgatgcgaa aacgaggcaa tatgccgctg taaaatagac tgccccaatc ccctcgagcc
    10561 gctatgaacc agcagttgca gatgctgcgc atccagacca gccagtgcaa acagttccgc
    10621 gtcgataatt tgatcaatct gttgcagttc tgcgaagtgg ttaccgccac cgatggagcc
    10681 aagcgagttg cgccacggat gctgtgcaaa tgctgacggc aggttttcct ccagccagct
    10741 ttcttcagca acgtcatcca gatctgataa tcgcttttca aacttatcgg cgttgtattt
    10801 gcgagcgaga atatctgttt gccatagcgc catgccgcag ccgatatcat tgccgaccag
    10861 tgccgggtaa aaacgaccaa cagagaataa cgctgcgcca atcgggtagc cgcgtccggg
    10921 gtgtaaatct ggcatcccta ctacgcgctg catgttgggt aaatttgccg tggtgtgtaa
    10981 ttgttggatc gctaaactct cgatccacag gtcatcagat gcgatggtaa ataccgcatc
    11041 ggataaggga cgaatatatt tgcccatgta ccattccatt tatgaataaa aatcaggaaa
    11101 tggcagacaa atccagggca agaaaaaaag aattaatcct ggaaaggtct gcaaagagtc
    11161 attgataaag gatttgtcat gatttacctc cttgcagttt gtggaataag aaaacgcgca
    11221 catactagac gggcgttatt ttcattgcaa gctggactta atgttgcggt ttatatcgca
    11281 tataaaaatt agtaaaccac gttccccggc attcaacgcg ctgctgctta actgtctgaa
    11341 aaccataacg ttcaaaaaag ggctttgcgg ttatgctggc gtccaccgta agttcggatt
    11401 cagacttaat caatggtttt aacaaagcgc tggcaacccc gcgtcgggtg tattccggtt
    11461 caacaaataa catatcgata taatgtccaa tgcaggtaat aaaaccaacc ggttgtgcat
    11521 taatgactgc aacccgcact tgtgatttcg cgagtttctc cttccagcga gattcgtcaa
    11581 tctgcgccca ggcggcaatt tgctgtggag aatagtgctg actcgccgtc atcgtaacag
    11641 ctcgaaggaa aatagcgcat agttgctgaa aatcaccagg ctgatagttt cttatttgta
    11701 tgttattcat aatcccagca ccagttgtct ttccatttgc gggtccagca acgtcacatg
    11761 cagccccacc agtcgcacac cgcgcccgcc gcggcgttca tcccaggttt tacgcgcggt
    11821 ggcgattaga tcagatttat tcagccgcgg ccagacgtgt tcctgggtgg tttgctgaaa
    11881 atcgtcgaac tttaatttca ccccctggcg ggcaatcagc aaatcaggtt ttacctttgc
    11941 caaacgacgc tccagttcag ggtaaagccg ctcgatgatc gcttcacatt cagaccagtg
    12001 atgaatatct tccgccatcg tacgttctac accgacggat tttcgcaacc tttcgctgtt
    12061 aacgtcgcgc tcgtcaatgc cctgactacg ctcccacaaa atgcggccaa atttgccaaa
    12121 acgtttgagc agagtcacca gatcacactt ttgcacatca ccgcaggttc gtagccccat
    12181 cgcttccagt ttagccgctg agactttgcc gacgccggga atttttgcca gcggtaaggt
    12241 ttgcagaaat gccggaacct cagccggagt aatcacaaac tgaccgttgg gtttattcat
    12301 gtcggaggcg attttggcga gaaacttgac cggcgcaacg cccgcagacg ccgtcagttg
    12361 cagctcgttg aagatcgtct ggcggatttc ctgggcgatg agggtcgcag aaccgtggca
    12421 atggacgcta tcggtgacat cgagataggc ttcatccagt gataacggtt caatgcgcga
    12481 ggtatagcgc gagaaaattt cgcggatatg atttgaggct tctttgtagg cgtcaaagcg
    12541 ccctggaagc aaggtgagat gcgggcataa tttgagcgcc atccctgtcg gcatagcgct
    12601 acgtacgcca aatttacgcg cgggataatt ggcggtgctg attacccccc gtcgttcgcg
    12661 gctgccgcca atagcaatag ggatatcgcg cagggcggga ttgtcgcgca tctccaccgc
    12721 tgcgaaaaag cagtccatat ccacatgaat gattttacgc attgctcacc tctcaacact
    12781 ggtaaagtat acagtgattt cagggtttga gaaatgcgta aagattcagc atttaacgcg
    12841 ctcgctgcga aagcacctgc tgcttatcca gcttttgcac cagcggctgc accactttat
    12901 ccccatgctg accaaagtat tgatacagcg tatcggacgc acttttggtc agcaccataa
    12961 tctcaatgcg ccggttgccc gcgctttgcg gatttttggc atccagcagc atctggtccg
    13021 ccattgcgct tacctgcatc actttatttt ccggcattcc ggcctcttcc agcacccgac
    13081 gagccgaaag cgcgcgatcg cccgaaaggt tccagttgtt gtagatattg tttttgtagg
    13141 ccatcgcatc ggtatgcccg gtaataataa ttttgttatc gagcgagtcg aacactggcg
    13201 caagctccac cagcaaagtt ttaaagaacg gcataatctt cgcgctgccg cgttcaaaca
    13261 tattgcggtt ctggtcgtct ttaatcagca cgcgtaatcc ctggggaaca atctccatct
    13321 ccagattcgc ttccatatgc gcatcacggg cgatggtatt gatactggtt gccagctcgc
    13381 caagctcggt ggcggatttc ttcttcaaca gcgccgtttt ctcgttgacg tcgcgcgctt
    13441 ttttctccgt ctcttccggc accgccacgg tggcaggttt tgacggatga gacgggctga
    13501 ttttatttaa cggcgacagc ccgccaccat tgaaaatcga ctgcccgtgc agcgcggcga
    13561 taatgctttc acgctcggat ttactgacgc tgttgacaat ccacagcgtc ataaatagcg
    13621 ccatcattgc caaagtaaag tcggcgaacg ccaccttcca cgccccgccg tgatgcccgg
    13681 catgattctt tttgatctgc cgccggacga tggtggtttt tgccccacgt tcgcggcggt
    13741 tgcccgtctt tctcatgctt attcttgctc cagcatcgca ttcacccacg cgtcgaggtt
    13801 ggcgaaggtc ggtttggacg ccagatgcag cagcttacgc ccggcgtcca ccgccagcag
    13861 cgtcggtttg ccgcctgcct gcgccaccag cacggtgcgc acgcattcca gcaaagaatg
    13921 ttccgcccgc gcctgttgtt ccatggcgtt ggcaagcggg tccatcaggc agtagcagat
    13981 aaacacgcct aaaaacgtcc ccaccagagc cgccgccact ttcaggccga tcagggcgat
    14041 agatccatca atcgactgca tggtgatgat gatgccgagc actgctgcac aaataccaaa
    14101 tcccggcatc gcttcggcgg tacgttgcag cgagcgtgac ggcgttaaca gcgactcttc
    14161 agcggtgtcc agttcctgat cgagaatgcc ttccagttca tgggcgtcaa tcttgcccat
    14221 cgccatcagg cgaaagttat cggcaataaa agtcaccagc cgcttctggg ttaacaccag
    14281 cgggtatttc tggaaaatgg tgctctcttc cggctgctca atatgttgat ccagcatacg
    14341 caggccgccg ttttgcacca tttcgagcaa ctcatacagg cacattaata gctgacgctg
    14401 aaactccggc ccgagctgtt ttttactgat cacgccttta atttgatggg caatctcttt
    14461 cagcacatgt ttcgggttcc caataatcat cgccccgaaa ccagcaccca aaataatgat
    14521 catttccgcc ggttgccaga tagccactaa ttgcccgccc gcttcaaaat aaccgccaaa
    14581 tacgcaaccg agcaccacca gtaaacctaa tattttttgc atggtttctt cactttaatt
    14641 cggatagaca aaatcacgaa ttttctgcgc gatctgttta ttcagttgac aaattcgggc
    14701 ctcggttagc cctaatacca gcgcaatctc tttgagattc atctcatgct ggtaatacat
    14761 ggagaggatc agttgctctt tttcgcttaa ttgtgaaagc gcgtgttgca acatttgctg
    14821 ggtgacaaac tgatcttcca gttcccggcc agcaagcgga atgcctggcc cttcgccgtt
    14881 cagcagttcg tccaggctgg cgagcgtttc cgccccttcc agttgctgat attcgagata
    14941 atcttcatgc gagatccctt cctcagccag ctcctgccac tccggttcgt gccccagttt
    15001 tttacgcgtt tcgcgaatca ggtctttcat ctgatgatat ttctgtcgca gctggcgcgg
    15061 tcgccagtcg agagcgcgca gctcatccag gatcgcaccg cgaatacggt gcacggcata
    15121 gcccgcaaaa ccttcgtccg gcagcccata gcgacgaatg gcgttcaaca gccccattaa
    15181 cgccgtttgt tccatatcct gacggtcaat aatgcaatta cactgcgggg caagttgccg
    15241 caccacttta cgcaccaacg gtaaataggc ctgcaagtag tggctctcct gctgcggagt
    15301 taataccagc gtggcagcaa attcttctga ctcgaataca tcctgtagca tgtttatatc
    15361 cgcctggtga ttactggaag accacttttt tcaccagcaa atctttatac ggtgcgctca
    15421 ttttgcgacg ctgaagatct ttctttaacg cgtcagaaat cagtttgcgg atggcagata
    15481 ttttcatgcc ccgcaaatct tcaaatttca tttccgtcag gcattcaacc gcaatacttt
    15541 gatacaacgg ctcctgatgc tttatttttt ccgcctcttt gtcactggcg accaccatca
    15601 ctaattccgc cgacaaataa tgatccgcgc cgttgttgtc atgcaaagtg acaatggttt
    15661 ccggcagcgt cacaaatacg ctcttgctct catccagagc ttccaccgat tccgtttgcg
    15721 cggcggtagt cggttttcct tttccggcat aatgatgcca gacactccat cctgctccag
    15781 cgccgaccac caacgccaga acggcgctga ccacgcctgc caccacgatt ttcttcatat
    15841 tgcctttaca ctttgatcaa aacggattcg ttctgttgaa attgaggcgc atcatcaagc
    15901 tgtaaggcgg cactcacctc ttcctgctga tggcccgact gttgttgctg ctgcgactgc
    15961 ccttcggagg agacctgtac gttcacctcc ataaaatttt gcgccgtcag atgctggcgg
    16021 agatcgtcgc taaactgttg taacgcgcgg caaacttcac tttggttcgc gccgatgtgc
    16081 accatcagtt tgccgttatc gagttgtacg gcgatctcaa gcttgccaag cgacggtgga
    16141 tcgagacgga tggtggagat ctgctgttgt ttgttgagct gaaagtggat ctgatctttc
    16201 aacagcgtcg tcagtttttc gcccagttcg tcgatcgaaa gcgtggctgg tttggcgaca
    16261 atctcctgat gatcgctgac cagctccggc gtcttaacct gcaacaccgc acgcgcaata
    16321 accggttcgc tgtccgacac tttttccgtt ttacgctcga cctgcgggcg cgcggtcaca
    16381 cgggttagtt caggtttcgg gcgagctggc tgtgttgaga cgcgcggctg cgtcggcgca
    16441 atggcatgta aatcttcact ggcgtaaatt gccagtctgg cctgctgttc gggtttcgcc
    16501 tgcggcaact gactgatcag ctcctgcaac tgcggcggca acggcgtgag ttcctgctgc
    16561 tgcgtcgggc gttgcgccgc gttttgcacc accgctttcg tcaattgctg gataaccggc
    16621 gatgttgcga cgtttcgcgg ctgcggcgtg acctgatgcg gcgcggcagg ttgcggaagt
    16681 aacagcgcca ttagcgcctg catcgccacc ggatcgttct cctgcgggcg cgtcgcctgt
    16741 tgctgactgg tttttgcgct atgaacgcgc tccgccaccg ccgcgaccgc cattttcggc
    16801 agggtaaacg cgggcgcgtt ttcactactc accggcggta aaatatccgc tttcagcgac
    16861 gccgtttccg caagtgttcc gagcgtggca agcaatgccg gattcatgac ttctccgaaa
    16921 gtaaattaac aattgagtag gccagtacgc cttcctgatg ccgctgatgc tgttccatac
    16981 gcgccttcaa cacttctgcg cgctgctcgc gccgtttaat cagggtatta aacgcgccgt
    17041 acaactgcgc tttcaccgta tccaactgcg ccgcatcggt ttccgccgcc gcgtaatcgt
    17101 gaaactggcg catcaactgc tgatgcagtg ccggaaggcg tcgccagcgt tgcttgtcga
    17161 gggcttcatt catcgcctcg accagtgcga aaagttgcct gcgttgtttt tccatcgtct
    17221 taacccagct tgcccgaaag cccttcccag ccttcgcgca ggttgcttaa gatcagcatc
    17281 acttcatcga ttttctctgc cgacagttcg ccgctggcct catacaatcg gtaaacgcag
    17341 tggtcataga ggcgcgaaag gttgaccacc agctcgccgc cagtttcaaa ctccagcgaa
    17401 ctggtgagcg cattgagaat gtcgatgcat ttattgatgc tctgggcttt cttctcgaaa
    17461 cgccgccctt caatatggct tctggcgcgt tccagctcgt ccatcaggcc agaaaacagc
    17521 accagcacca gttccagagg cgatgccgcc gcagtacgcg ccgcgagatc gatctcttta
    17581 tattgcgaat agccgtcctg tacttggtac atctttaggg atattccttc tgagttacgc
    17641 gaatgccgcc atgctggctt tcatggtgta cacctcgacc agcgtgttgg tgtactcctc
    17701 gagataacgg tcgtagttgg cgttataggt atcagtaagc tgatcgcctt cgtcctgaat
    17761 tttgctcatc tgatcgtcaa tgttctgctg gcgcagggtg atgatgccgt tgctggagtc
    17821 cgtgtaggtg ttaatcaggt cgtccatctg tgccaccatg ctgttgtcgc caacgaaaat
    17881 agaggtcaga ccgtcaggat ttttcgccat ttcgtcgttg aactggtcgg agtcaatctg
    17941 taagtggccg tgagaatcaa gcgtgatgcc atagtcaaca atcgacacgc cgttgtagct
    18001 ggcatgggcg atgtcatcga gctggttcgc cagtgaactg agtccggcgt cgcctgcaaa
    18061 taccccggcg ctggtactgt catcaccgtg agtggttagc gaatcaaccg tatcgatcag
    18121 ggtgttatag gcatcgacaa aggtctgcac tttctcctgg ctggcgctgg aatcttcgct
    18181 aatgttaaag gtggtgagat cgctgtcaga atcgctgact tcggtgaaag tcatggtgac
    18241 gccggggatc acatcatcaa acgtattgct gctgttggtg atctcaggcc ccgtggcgct
    18301 gccaagatga ataatcgcgt cctgagcgga ggaaacatcc gtggcaacgg gcgcgctggt
    18361 gctatcgttg ctggtgtcat gccccgttac gctcaccgaa aacgcgctct gcgccccggt
    18421 gctatccgag gtgagcataa tcgtggtggt gccgtcggtt tttaccagtg ccgccgacac
    18481 gcccggattg tcatcagagt cgttaatcgc attcaccagt tcactggcgt cgataaaacc
    18541 gtcgccgtca ccgttctgat ccgccaccga cagatcaata tccatggtgc tgtcgcccat
    18601 cgtcagttca aaagtgccgg tggcggaaaa ggcgtcgtcg cccatactga acgtggtttg
    18661 ctgcccctgc gccagttgct caacgaaaaa cgagtagctt cccgcctgtg cctgcgagtt
    18721 agcggaaacg gtcgccgagt cattattgct ggtggcggca aaagtcaccg ggccgtcggt
    18781 atcgctgttc agcgcgtcga cggcgctctg gaaatcgctc aacgctgagc tgagcgaatc
    18841 caggccgctg ctttcagcat ccagctccgt ttgcttttcc tgcaagtttg ccgcctgggt
    18901 ggcaacatcc gcatacgcga tttcttgcgc aatggttctt gggttgatca ttgatgactc
    18961 cggataacaa tgtcaggtta ttggcaaaag gcgtgccaat attttttatg tgaataatca
    19021 atagattgaa aaacaggcgg aaatgacgtt tccggtctgg cggaaaaaag cctgccgcag
    19081 aaagcacaac accgcccaca ggcggtgttg ttcgtggccg gataaggcgt ttacgccgca
    19141 tccggctatc ggtgcacagt gcctgatgcg acgctgacgc gtcttatcag gcctacgggt
    19201 cca
//
LOCUS       DAZFJL010000089        18844 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFJL010000089 DAZFJL010000000
VERSION     DAZFJL010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.089"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(46..954)
                     /gene="galU"
                     /locus_tag="ACHJ8C_003094"
     CDS             complement(46..954)
                     /gene="galU"
                     /locus_tag="ACHJ8C_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO5552906.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEDEMGIKK"
     gene            complement(1156..2169)
                     /gene="rssB"
                     /locus_tag="ACHJ8C_003095"
     CDS             complement(1156..2169)
                     /gene="rssB"
                     /locus_tag="ACHJ8C_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO5552907.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISQCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(2261..3166)
                     /gene="rssA"
                     /locus_tag="ACHJ8C_003096"
     CDS             complement(2261..3166)
                     /gene="rssA"
                     /locus_tag="ACHJ8C_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO5552908.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            3279..3737
                     /locus_tag="ACHJ8C_003097"
     CDS             3279..3737
                     /locus_tag="ACHJ8C_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO5552909.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDGGNIGFVSFVAQFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            3787..4629
                     /gene="purU"
                     /locus_tag="ACHJ8C_003098"
     CDS             3787..4629
                     /gene="purU"
                     /locus_tag="ACHJ8C_003098"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO5552910.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            4789..4873
                     /locus_tag="ACHJ8C_003099"
     tRNA            4789..4873
                     /locus_tag="ACHJ8C_003099"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4823..4825,aa:Tyr,seq:gta)
     gene            4908..4992
                     /locus_tag="ACHJ8C_003100"
     tRNA            4908..4992
                     /locus_tag="ACHJ8C_003100"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4942..4944,aa:Tyr,seq:gta)
     gene            complement(5009..6100)
                     /locus_tag="ACHJ8C_003101"
     CDS             complement(5009..6100)
                     /locus_tag="ACHJ8C_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HFO5552911.1"
                     /translation="MNLFNPDRLITFPADDPQLVKQLQSGTKVYLAKSGDHRYADGWA
                     FVKMAVLIIACFSCYLLALFQRQWGLYLLWYLAMMFCAMLLAVNVVHDASHDAFLRGK
                     KTNARLNRWVAFPMGLDPDCWRVRHVRFHHGFTNIEFYDPDTAENGILRQTPWQRWRP
                     FMRQQHHYWPLVAALTFPWYIWVVDWLDRAGFTPVTRHLALRGFAGWGYFLAGKLAHC
                     ALCLILPWLLSDFSFMTILLTYLLSQLLASLIFVTLIIGTHWAKGHTQLPPEEGKMPV
                     GRLAHTFVTTFDWTPQPAWLGYWLGGINLHLTHHLFPHWHHRHYPALSRIIAQIASQQ
                     GLDYQLLTLADLLRLQQQFLRRMGEKPKA"
     gene            complement(6087..7211)
                     /locus_tag="ACHJ8C_003102"
     CDS             complement(6087..7211)
                     /locus_tag="ACHJ8C_003102"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HFO5552912.1"
                     /translation="MSDLLFPIISMVLLVLAEAGVLQFTGRQKVDWLDVIFNINSGHM
                     MLWLFRCLEVLCYGLVVNHFSLGLFDNAPVILLWLFTILAWDFGFYWLHRWHHEMRPL
                     WAVHVVHHQGEHYNLSLGVRNSWYSSLTSIPFFMLLAMLGVPLQIFLAVSIIHYSWQF
                     FNHNALTPKLGWLEKVLITPSHHRVHHLNQKQYADTNYGGTFLLWDKLFGSYSAGPEY
                     DVGYGVKNSRLSCNPMRESNLPFLRLMGFRSFDKAIEGRYQSSPWAVGAGVILLFMLV
                     LGYIQQYGYGIEFITSEQAVLFVLLAAGSVALGGMSDGQHWGMILWCSVTLSLLYFIV
                     YMCSWSTLLWKGLVGILLAHCLLFVIGVGRKSLRKQYEPV"
     gene            complement(7204..7806)
                     /locus_tag="ACHJ8C_003103"
     CDS             complement(7204..7806)
                     /locus_tag="ACHJ8C_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002432219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFO5552913.1"
                     /translation="MSNSLLRLKQMLLGWGTVGVIYNFSDYLQGEGYQLTPSIIDNWI
                     SFSPSAVWLYLSFFFIVPLGYLSTPLCHLRWLARSMQISALVAGVFYLLWPTTMHYPS
                     FDQMGISAGALNRLIAIDSSQNCFPSLHAALTLLAVWAIAKKGNRWLTLMSVVWAIAI
                     AFSILQLRRHLFIDLVGGALLASICGWICATCEKRKTQYE"
     gene            complement(7803..9089)
                     /locus_tag="ACHJ8C_003104"
     CDS             complement(7803..9089)
                     /locus_tag="ACHJ8C_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F390 synthetase-related protein"
                     /protein_id="HFO5552914.1"
                     /translation="MIPLALLWRYFRTRKLHFTDREALENWQAKRLHQFRQNVLSHSP
                     WFQRYLTLPFNQWPMMDKALMMTHFDEMNTAGLKRDELLDCAMRSEQSRDFKPCVGRF
                     SVGLSSGTSGRRGLFVVSPHEQQMWAAGVLAKVLPDGLFAKERVALFLRADNNLYQSV
                     NNRWLSLDFYDLLAPFQAQLKLLQQRAPTIIVAPAQVLRALALEVMAGELTLKVKKVI
                     SVAEVLEPQDRELLRNVFHNVGEIYQATEGFLASTCRCGTLHLNEEFVHIEPQWLDEH
                     RFVPIVTDFTRTTQPIVRYRLDDVLVASEQPCSCGSATMAIARIEGRQDDQLQLPTRS
                     GDIQTVFADACSRVLAMTLPLTVDYRLLQTGSAQLTLIADCELWMLEHCREALNALFI
                     RQDIAVGQLIWSLEPQTVPVSFTAKRRRIVRQWGRE"
     gene            complement(9086..9892)
                     /locus_tag="ACHJ8C_003105"
     CDS             complement(9086..9892)
                     /locus_tag="ACHJ8C_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFO5552915.1"
                     /translation="MAKITAFETGWCTHISCIALKGAGLKVCKFPARAWLLEVGDKRW
                     LWDTGYSSWFEHYTQSGVFRLYRQVTPVYFDPQQALVRQLSATGLAAKDVDAIILSHF
                     HADHIAGLRDFPDVSCICSGEGWRQVRTLRGVTALRQAFVPGLIPENFDSSLRFIESF
                     ASVNLPAQLAPFECGYLLPESNGEIILVPLPGHAAGHIGAFVLTDNGWTLLAGDAAWA
                     PANYRELRGPSPLAHLVMSDSHAYYHTLGQLHQLWQNGIVEIHLCHEGDL"
     gene            complement(9870..10886)
                     /locus_tag="ACHJ8C_003106"
     CDS             complement(9870..10886)
                     /locus_tag="ACHJ8C_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO5552916.1"
                     /translation="MKILVTGATSGLGRNAVQYLLQSGESVVATGRNCEVGRSLTNAG
                     AQFVALDLTEASEDDCARLMDGCDAVWHCAAKSSPWGDKTAFWLANVKVTHTLAQAAG
                     RLNIPRFIHISTPAVYFDFQHHNDLPETYLARAFSSHYASSKYAAEQVIAGAVSRYSA
                     TRFILLRPRGLFGPHDQVIVPRLMQQLQQGGGCLRLPRGGDALLDLTFVQNVVYAMRL
                     ATNVEGLRSGSIYNISNHQPQQLSVMLDALLHQQLGLSYRIAAVPWSLLSLVARGLEL
                     YGKCVNQEPVITRYSAGTLCFDMTLSAQKAITELGYRPRFSMEQGIAITGQWLREHGK
                     NNRI"
     gene            complement(10883..11890)
                     /locus_tag="ACHJ8C_003107"
     CDS             complement(10883..11890)
                     /locus_tag="ACHJ8C_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HFO5552917.1"
                     /translation="MARPNQPTISAALKIIATGIALPPDQITSQQLDVQLNKPVGYVE
                     RRSGICWRYFARNDASQAELAAEALHNALVAAALNPTDIDLLICASAIAVQALPCSAI
                     HILKAAAMPAGVAGFDINSSCVSFISALEVAAGLLNCGAYRRIAIVSADIASRGIDWD
                     HEESSLIFGDGAACAIVERGDGRSGIIASLIEMYPEGSELCEIRAGGTRRNPRSGVSD
                     SDFLFHMQGKPLFRFASSLIEDYFSRLLQKSSLQLDDIGTVVPHQASHLSLEHMRKRL
                     RVPESALIDVYRFYGNQVAASIPTALHEAVISGRFTADKPVMLIGTAAGLTLAGMVLL
                     P"
     gene            complement(12002..12679)
                     /gene="narI"
                     /locus_tag="ACHJ8C_003108"
     CDS             complement(12002..12679)
                     /gene="narI"
                     /locus_tag="ACHJ8C_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO5552918.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(12679..13389)
                     /gene="narJ"
                     /locus_tag="ACHJ8C_003109"
     CDS             complement(12679..13389)
                     /gene="narJ"
                     /locus_tag="ACHJ8C_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO5552919.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFAEKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(13386..14924)
                     /gene="narH"
                     /locus_tag="ACHJ8C_003110"
     CDS             complement(13386..14924)
                     /gene="narH"
                     /locus_tag="ACHJ8C_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO5552920.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTICSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(14921..18664)
                     /locus_tag="ACHJ8C_003111"
     CDS             complement(14921..18664)
                     /locus_tag="ACHJ8C_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO5552921.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAREAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         4693 a         5127 c         4624 g         4400 t
ORIGIN      
        1 cgtcgattgc tcaacgccgt ttcgtggaaa acaccgatac ggatgttact tcttaatgcc
       61 catctcgtct tcaagccagg ctttaaattc cgtgccaagg gtgttatgac gaataccgta
      121 ttcaacgaag gcctgcatgt aacctaattt attaccgcag tcatggctct tccctttcat
      181 atgataggct tcaaccgttt ctttttcgat cagcatatca attgcgtcgg tgagctgaat
      241 ttcatcacca gctccaggag gggtttttgc cagcaacggc caaatatccg cgctaagtac
      301 gtaacgaccc acaatagcga gattagacgg cgcaacatcc gcttttggtt tttcaaccac
      361 gccaaccatc ggtacgcttt cacccggcgc taattcaacg cctttgcaat ccacaacgcc
      421 atatgcggtc acatcagcaa ccggttcaac catgatctgg ctatgacccg tttcatcaaa
      481 gcggcggatc atctctgcca ggttatcctg tgataaatcg gattcatatt catccagaat
      541 aacgtcaggc aaaataacag ccacaggttc atcacccact accgggtgag cacacaatac
      601 cgcgtgtccc aggcctttcg ccagcccctg acgaacttgc ataatagtca cgtgcggtgg
      661 acaaatagac tgcacttcat caagcagttg acgttttaca cgtttttcca gcattgcttc
      721 cagttcaaaa ctggtatcaa agtggttttc aatagagttt ttagatgagt gagtaaccag
      781 cacaatttca gtaatgccag ccgcaataca ttcattcacg acgtattgaa ttaatggctt
      841 atcgacaagt ggcagcatct ctttcgggat ggctttcgtc gccggcaaca tcctggttcc
      901 taatcccgct acggggataa cggctttttt gactttcgta ttaatggcag ccatttaaat
      961 tctcctggac tgttcgtgtt ttgaacgtgt tcatatttct gtatcgcatc ccagtatatc
     1021 agcaccccga aggggctaag ttctgaaaag gaggcgagtt agcataatta agacaattac
     1081 ttatacgaat agcgacgata gtaccaccag ccgacattag caggtaatgc aaatttagcc
     1141 cgtgttatcg tttgctcatt ctgcagacaa catcaagcgc agccgaccac cggttcccca
     1201 tatttggcat tgccaggcat cgcatcgctg gctcaattga ttcaaataag cgttacctaa
     1261 agtgcctaac ggaacaccat tactgatttg cacctgatgt tcgccggtat ttaacgtcgc
     1321 attcagaccc gcagaaacca gaatgagatt tttcagttcg cgatgataat agccaactaa
     1381 tagcggaaac tgccccggca gattggcctg acggagtaaa tggtttacct gcttcaataa
     1441 cgcgcccaac tctggcaacc gttgattttg gtgtgcaagc tgttcctgta ataatccgtt
     1501 aaacaatgcg cgtaataaca aggcagcaag tacgccatta tgtccagctc gggtgacatc
     1561 aaggcaataa aatgccagat cgttttccga aagtgcggca atatcaagca ccaggccggg
     1621 tttgtccgcg gcaactaatt gacgataatt aacccggcat tgggaaatca cctgctgaac
     1681 tggcggttgt agttcctgta ataatttcgc cgctgcagca gggttatcaa ccattgcatc
     1741 ccagtcgcga aaaagccttt cctcttcctc aacgcgggaa ttaaacatgc tgggatagag
     1801 acaggcaaaa accatctcgc gcaagcgatt cagatcttta accggtttca gcaaaacatc
     1861 ttcaacgccc agacgtaacg ctttggcaat atctgccata ttttcagtgg cagatatcac
     1921 cagaactggt gtctggtcac ctctgttgcg tatatgttcc agcagtttaa gcccgttcat
     1981 tcgtggcatc gcgatatcac atatcatcag gtctggagtg aaacctccca gcaactcaag
     2041 ggcatccacc ccatcagccg ccagtaccgt tgtcgctccc aatgaggaaa accacgaatc
     2101 cagaagcgag cgaaatacct gctcatcttc aacaatgaga atctgttttc cgaccaatgg
     2161 ctgcgtcatg ttctctcccc tgactggctt tactcaatag tggcacgcta ttgccaccta
     2221 cgcctgtcag aattaactta agtgtagata aaaattctgg tcaaatgttg gtgcgtacca
     2281 acggcaaaag ttcgtccatt ttcttttcca ctgccagctg tccggccgca atggcagcgt
     2341 gcgcgcgatg gaaatcaagc gtagatattt gcgggcaaac aggttgaatc agaatatcgg
     2401 gcggatcacc tgccatgcgg ttccttttaa ggcggttctc cagcacctgg atagaagtgg
     2461 tcataatctc tgttgccgtt ggcgctgtca ccgcccgacg tgtcgttatg ctgcctaacc
     2521 tttctttcag acgcgcatgc cacggcagag aatcaccatt ctcgctattt tcttcactga
     2581 cattaaagga gagcaaatct tgttgcatca aatgagcatc gtgctgcagg tcgaccgcta
     2641 tcacaatatc agcccccaat gcacgcgtga gggaaatagg aattgggtta actacagcgc
     2701 catcaaccag ccagtagccg ttatgcgcaa cgggtgccat tagtcctgga atactgcatg
     2761 atgcacgaat agcaagatgg agatcgcctt cagtaaacca taattcacgc ccagtactta
     2821 aattggtggc aacagccgcg aagcgacggg aacaattttc gatctctgtt tccggcatta
     2881 tttcgcgata ttgattgaag acacgctcgc cgcgcagtaa cccaccgcgc tgccaggaga
     2941 gatccatcag gcgtaaaaca tcccaataac tgaaagaggt cacccaatct tccagcgcag
     3001 ataatcgatc gcatgcatag gcagcgccca ccagcgaacc aattgaacat cctgcaacga
     3061 tatcaatttc aatacccact ttttttagcg cattaattac gccaatatgc gaccaacctc
     3121 ttgccgcgcc agatcccagc gccagcccta tttttatctt tctcattatt cccgcaagat
     3181 tcccctggaa tgcaatcgta gccacatcgc tactgctcgg ttaacatagt gctactcagg
     3241 cggttaatac gccgttattg tttccaggga gattatttgt gtctcagctt tgtccctgtg
     3301 gtagtgctgt cgagtatagc ctatgttgcc acccttatgt gtctggtgaa aaggttgcac
     3361 ctgatccaga acatctcatg cgttcgcgtt actgcgcttt tgtgatgcaa gacgcagatt
     3421 atttaataaa gacctggcat ccatcttgtg gagcagcggc gttacgtgcc gaattgattg
     3481 ccggatttgc gcatactgaa tggttgggat tgaccgtatt tgaacattgc tggcaggacg
     3541 gcggcaatat cggatttgtc agttttgttg ctcaatttac cgagggtggc aaaacaggtg
     3601 caataattga acgttcgcga tttttgaaag aaaacggtca atggtactat attgacggta
     3661 cacgtccgca gtttggccgc aacgatccct gcccttgtgg ttcaggtaaa aaatttaaaa
     3721 agtgctgcgg ccaataatgg ttgacggtac ggtttagcaa acactctcaa caaggttttt
     3781 ccagcaatgc attcactcca acgtaaagtt ctgcgtacta tttgtccgga ccaaaaaggt
     3841 ctgatcgcac gtattaccaa tatttgctac aagcacgagt taaatatcgt gcagaacaat
     3901 gaatttgtag atcatcgtac cgggcgcttt tttatgcgca cggaactgga agggattttt
     3961 aatgattcca ccctgctggc ggatctcgat agcgcattgc cagaaggctc cgtgcgtgag
     4021 ctgaatcctg ccggtcgtcg ccggatagtg attctggtca ctaaagaagc gcattgcctt
     4081 ggcgatttgt tgatgaaagc caattacggc ggcctggatg tcgaaatcgc ggcagttatt
     4141 ggtaaccacg atactttacg ttctctggtt gagcgttttg atattccgtt tgagctggta
     4201 agccatgaag ggttaagccg caacgagcac gatcaaaaga tggcggatgc cattgatgct
     4261 tatcaacctg actatgtggt gctggcgaag tatatgcggg tattaacgcc ggaatttgtg
     4321 gcacgcttcc cgaataagat catcaatatt caccattcat tcctgccagc gtttatcggc
     4381 gcacgtcctt atcaccaggc ctatgagcgt ggcgtgaaga ttattggcgc aaccgctcac
     4441 tatgtgaatg acaatctgga cgaaggccca atcatcatgc aggacgttat tcatgtcgat
     4501 catacctaca cagctgaaga tatgatgcgc gcaggtcgtg acgtcgagaa aaacgtctta
     4561 agtcgtgcgc tatacaaagt actggcgcag cgcgtctttg tttacggtaa tcggacgatt
     4621 attctttaat cgccagcaaa aataactggt tacctttaat ccgttacgga tgaaaattac
     4681 gcaaccagtt catttttctc aatgtaacac tttacagcgg cgcgtcattt gatatgatgc
     4741 gccccgcttc ccgataaggg agcaggccag taaaaagcat taccccgtgg tggggttccc
     4801 gagcggccaa agggagcaga ctgtaaatct gccgtcatcg acttcgaagg ttcgaatcct
     4861 tcccccacca ccatctcaag cattacctca aaatttgaat tacccctggt ggggttcccg
     4921 agcggccaaa gggagcagac tgtaaatctg ccgtcataga cttcgaaggt tcgaatcctt
     4981 cccccaccac catcacttga ttccattatc aagccttcgg cttctccccc attcgccgta
     5041 aaaactgctg ctgtagcctc aacaaatccg ctaacgtcaa caactgataa tccagacctt
     5101 gctggctggc aatttgtgca ataatgcggc ttagcgccgg ataatggcga tgatgccagt
     5161 gaggaaacag atgatgggta aggtgcagat taatcccgcc cagccagtag ccaagccatg
     5221 caggttgcgg cgtccagtca aaggtggtaa caaaggtatg cgccagtctg cccacgggca
     5281 ttttgccctc ttctggtggt aactgcgtat gtcccttcgc ccagtgcgtt ccaataatca
     5341 acgtcacaaa aatcaatgac gccagcagct ggctgagcag ataagtcagc aggatggtca
     5401 taaaactaaa atcagacaaa agccagggaa gtatcagaca cagcgcacaa tgcgccagtt
     5461 tcccggctaa gaaatatccc cagccagcaa agcctcgcag ggccagatgt cgcgtgaccg
     5521 gcgtaaaccc tgcgcgatcg agccagtcca ctacccagat ataccaggga aaggtgagcg
     5581 cggcgaccag tggccaatag tgatgttgct ggcgcataaa aggtcgccac cgttgccacg
     5641 gcgtctggcg aagaatgcca ttttctgctg tgtctggatc gtaaaattca atattggtaa
     5701 aaccgtgatg aaagcgaacg tggcgcaccc gccagcagtc cggatctagc cccataggaa
     5761 aagcaaccca ccggttaagt cgggcattgg tctttttacc acgcaaaaac gcatcatgcg
     5821 aagcgtcatg caccacattg accgctaaca acatggcgca aaacatcatc gcgagatacc
     5881 acaacagata cagtccccat tgacgttgaa acaacgccag taagtaacag gagaaacaag
     5941 caataatcaa caccgccatt ttgacgaatg cccatccgtc ggcataccga tgatcgcctg
     6001 atttcgccag ataaactttc gtaccgcttt gcagttgttt caccagttgt ggatcatcgg
     6061 caggaaaagt gattaacctg tcgggattaa acaggttcat actgtttcct tagcgattta
     6121 cgtcctacgc cgattacaaa taacaggcaa tgggccagca gaatacccac taatcccttc
     6181 cagagcagag tactccatga acacatatag acaataaaat agagcagtga caacgtcaca
     6241 gagcaccaca aaatcatacc ccaatgctgc ccgtcgctca tgccccccag cgcaacggag
     6301 cctgccgcca gcagaacaaa taatacggct tgctcgctgg taataaactc aatgccatag
     6361 ccatattgtt gaatgtagcc aagcaccagc ataaacagca ggatcacccc tgcccctacc
     6421 gcccatggag aactctggta gcgaccttca atcgctttgt caaatgagcg aaagcccatt
     6481 aagcgtaaaa atggcagatt actctcgcgc atcgggttac aggacaagcg actatttttt
     6541 acgccgtagc ctacgtcgta ttccggcccg gcactatacg aaccgaaaag tttatcccac
     6601 aacaaaaagg tgccaccgta attggtatcg gcatattgct tttgattcag atgatggacg
     6661 cgatgatgcg aaggcgtgat cagcactttt tccagccagc ccagtttcgg cgttagtgcg
     6721 ttatggttaa aaaattgcca actatagtga ataatagaaa ccgccaaaaa gatctgcaac
     6781 ggcacaccaa gcatcgccag cagcataaaa aacgggattg aggttaacga ggagtaccac
     6841 gagttccgca cgcctaatga caggttgtag tgttccccct gatgatgcac cacatgtacc
     6901 gcccacagcg ggcgcatttc gtgatgccag cgatgcagcc agtaaaagcc gaaatcccac
     6961 gccaggatag taaacaacca cagcaggatc accggcgcat tatcaaacag tccaaggctg
     7021 aagtgattga ccaccaggcc atagcagagc acttccaggc agcgaaataa ccacagcatc
     7081 atatgaccgg agttgatatt aaaaataacg tccaaccagt ccactttttg ccgcccggtg
     7141 aattgcaaca cgcctgcttc ggctaacacc aacagcacca tcgatatgat ggggaataat
     7201 aaatcactca tattgtgtct tccttttctc gcatgttgcg catatccaac cgcaaatcga
     7261 cgccagcaac gcgccgccta ctaaatcgat aaataaatgt ctgcgtaatt gcagaatcga
     7321 aaaggcgatt gctatcgccc agaccacgct cattagcgtc agccaacggt tccccttttt
     7381 ggcaatcgcc cataccgcca acagcgttag cgcggcgtgt aaggagggga aacagttctg
     7441 gcttgagtca atggcgatca gcctgttgag tgcgcccgcg gatatcccca tctgatcaaa
     7501 cgagggataa tgcatggttg tgggccagag aagataaaat acgcctgcca ccagtgcgga
     7561 aatttgcatt gaacgggcca gccagcgcag gtggcaaagt ggcgtggata aataacctaa
     7621 cggcacaatg aaaaaaaacg agagatacag ccacacagcg gagggggaaa acgaaatcca
     7681 attatcaatt attgacggtg ttaattgata tccctcacct tgcaggtagt cgctaaagtt
     7741 gtagataacc ccaacagttc cccagcccag cagcatctgt ttcaggcgta gcagtgaatt
     7801 gctcattccc gcccccactg acgcacaatg cgccgccgct tggctgtaaa agagactggc
     7861 acagtttgcg gttccagcga ccaaattaat tgcccgacag cgatatcctg gcgtataaac
     7921 aaggcattca acgcctcccg gcaatgctcc agcatccaca gctcgcaatc ggcgattaac
     7981 gtcaattgtg ctgacccggt ttgtagcagg cgataatcga ctgttagcgg cagtgtcatt
     8041 gccagcacac gactacaggc atcggcaaaa acggtctgaa tatcgccgga tcttgttggt
     8101 agttgcagtt ggtcatcctg ccgcccttcg atacgcgcaa tcgccattgt tgcactgcca
     8161 caggagcatg gttgttcgct tgccaccagc acatcatcca gtcgataacg cactatcggc
     8221 tgtgtggtgc gggtaaagtc tgtgacgatc ggcacgaaac ggtgctcatc cagccattgc
     8281 ggttcaatgt gaacaaactc ttcgttgaga tgtaacgttc cacaccggca ggtggaagcg
     8341 agaaagcctt ctgtcgcctg ataaatttcc cccacattgt ggaacacgtt gcgcagtaat
     8401 tcccgatcct gcggctccag aacttccgcc accgaaatca cttttttgac ctttagcgtc
     8461 aattcgccgg ccatcacctc cagcgccagt gcgcgtaata cctgagctgg cgcgacaata
     8521 atcgtgggcg ctcgctgttg gagaagtttg agttgcgcct gaaatggcgc tagcagatcg
     8581 taaaaatcga gacttaacca gcgattattc acgctttgat acaggttgtt gtccgcgcgc
     8641 agaaacagcg ccacccgctc tttggcaaat aaaccgtcgg gcaacacttt tgccagcaca
     8701 ccggcggccc acatctgttg ctcgtgcggg ctaacaacaa aaagcccacg acgacccgat
     8761 gtacctgatg aaagcccaac actgaacctg ccaacgcagg gtttgaaatc acgactctgc
     8821 tcactgcgca ttgcacaatc gagaagttcg tcgcgcttta aacctgcggt attcatctca
     8881 tcgaagtgcg tcatcattaa cgctttatcc atcattggcc actgattaaa gggcaacgtc
     8941 agataacgct gaaaccacgg gctatgagat aagacatttt gccggaactg atgcagcctt
     9001 tttgcctgcc agttttccag tgcctcgcga tcggtgaaat gcagcttgcg ggtacgaaag
     9061 tagcgccaga gtaaagccaa cgggatcata aatccccctc atggcacaaa tgaatctcca
     9121 ctataccgtt ttgccaaagc tgatggagtt gcccaagcgt gtggtagtag gcgtggctgt
     9181 cggacataac cagatgcgcc agtggtgaag gccctcgcag ctcccggtag ttagctggcg
     9241 cccatgccgc atcgccagcc agtaaagtcc agccgttgtc ggttaacaca aacgcgccaa
     9301 tatgtccggc agcatgtccg ggaagcggca cgagaataat ttcgccattg ctttctggca
     9361 gcaaatagcc gcactcaaac ggtgccagtt gcgcgggcaa attcaccgat gcgaaacttt
     9421 caataaagcg cagtgaggag tcaaaattct ccgggattag ccctggcaca aaggcctggc
     9481 gcagcgcagt gacaccacgc agagttctta cttgccgcca gccttcacca gaacagatgc
     9541 aggaaacatc agggaaatca cgcagaccag caatatgatc agcgtgaaaa tgcgacagaa
     9601 taatcgcatc aacatctttc gctgcgaggc cggtggcaga aagttgccgc accagcgcct
     9661 gttgcggatc gaaataaacc ggcgtgacct gacgataaag acgaaacaca ccagattgcg
     9721 tgtagtgttc gaaccacgaa gaataaccag tgtcccacag ccagcgttta tcgcccacct
     9781 ccagcagcca ggcgcgggcg gggaatttgc acactttcag ccctgcccct ttcagcgcaa
     9841 tacagctaat atgggtacac cagcccgttt caaatgcggt tatttttgcc atgttccctc
     9901 agccattgtc ccgtgattgc aataccttgt tccatcgaaa agcgcgggcg atatcccagc
     9961 tctgtaatgg ccttttgcgc actcagcgtc atatcgaaac agagtgttcc ggcgctgtat
    10021 cgtgtgataa caggttcctg attaacgcac ttcccgtaca gttccaggcc acgcgcgacc
    10081 agagaaagca gcgaccaggg aacagcagca atgcggtaac tcaagcccag ttgctggtgc
    10141 agcaaggcgt caagcatcac cgatagctgc tgcggctggt gattgctgat gttgtaaata
    10201 ctgcccgagc gtaagccttc aacgttcgtt gccagccgca tggcatacac cacgttttgc
    10261 acgaacgtga gatccaataa cgcgtcgccc ccgcgcggca aacgtaagca accaccgccc
    10321 tgttgcaatt gctgcatcag acgaggaaca attacctggt catgggggcc aaacaaacca
    10381 cggggacgca aaagaataaa tctcgtggca gaatatcgag aaacagcgcc agcaataacc
    10441 tgctcagcgg catatttact gctggcataa tggctggaga atgctcgcgc aagatacgtt
    10501 tctggcaggt cattgtgatg ttgaaaatca aagtaaaccg caggcgtgga gatatggata
    10561 aaccggggaa tattcagcct gcctgccgcc tgtgccagag tgtgggtcac tttgacattg
    10621 gcaagccaga acgccgtttt gtcgccccag ggcgaggact ttgccgcaca gtgccagacg
    10681 gcatcgcagc cgtccatcag gcgggcgcag tcatcctcgg atgcctcggt taaatcgaga
    10741 gccacaaact gtgcgcctgc gtttgtcagg cttctgccaa cctcacaatt tcgccctgtt
    10801 gccacaaccg actcacccga ttgcaacaaa tattgcacgg catttcgccc aaggccgctg
    10861 gtcgcccctg ttacgaggat cttcacggta aaagcaccat gccagccagc gttagtcccg
    10921 ctgccgtgcc aatcaacatt acaggtttat ccgctgtgaa acgaccgcta atcactgctt
    10981 catgcagcgc cgtggggata gaggccgcga cctgattgcc gtaaaaacga tacacatcga
    11041 tgagcgcaga ttccggcact cgcagccgtt tacgcatatg ttcaagggaa agatgactgg
    11101 cctgatgtgg aacgaccgtg ccgatatcat ccagttgcag gctgcttttt tgcagcaacc
    11161 gagaaaaata atcttcaata agcgaagaag caaaacgaaa cagcggtttc ccctgcatat
    11221 gaaaaagaaa gtcgctatca ctcacaccag aacgaggatt acgccgcgta ccgccggcgc
    11281 gtatttcaca cagttcgctg ccttcgggat acatttcgat caggctagcg ataatcccgc
    11341 tgcgcccatc gccccgctca acaatcgcgc acgccgcgcc atcaccaaaa attaatgaag
    11401 actcttcatg atcccagtcg atacctcgtg aagcaatatc tgccgaaacg atggcaatgc
    11461 gccgatatgc tccacagttt aataatccgg cagcgacttc aagagcggaa ataaagctca
    11521 cacagctact gttgatatca aacccggcaa cgcctgcggg catggctgcg gctttaagaa
    11581 tatgaattgc gctacagggt aatgcctgta cggcaatggc cgatgcacaa attagcagat
    11641 caatatcagt aggatttaag gctgccgcta ctaacgcatt atgtaaagcc tctgccgcca
    11701 gttcggcctg gctggcgtcg ttgcgggcaa aatagcgcca gcaaataccg gaacgtcgct
    11761 caacatagcc aactggctta tttaactgaa catccagttg ttgagaagtg atttgatcag
    11821 ggggtaaggc gatccccgtg gcgattattt ttaacgccgc ggagatcgtc ggttgattag
    11881 gtcgagccac ggtcagtgct ccttcactgc tccataggtg gaccaatcat atagatgaag
    11941 tacgtttccc tacaatccga aaaatcggca gtgtgtaacc accgatgttt ggtttagata
    12001 attagtgacg agcgcgcacc agctgatact tacgtgtcag atactccacc gggacgctcc
    12061 agatgtgtac cagacgcgag aacgggaaca gcaggaataa cgtcatcccc aacaccaggt
    12121 gcagacggaa gataaacgcc acgccatcaa gatgttgaga agcgccaccg tggaaggtca
    12181 ctaccgactg cgcccagcca accagtttca tcatctcgct accgtccata tgctgggcgg
    12241 agaacggaat ggtcagcagg cccagcgcgc actggataac gagcagcgac aggatcagga
    12301 tatccgctcc ggtagtcgtt gcacgcacgc gtgggctgaa cagacgacgt ttcaacaaca
    12361 gcacgccgcc aatcagacac agtacgccgc tggcaccacc agcaaacatc gccattttct
    12421 gttttacttc aatcggcagc caggcttcat acatccagtg cggcgtcagc ataccgaaga
    12481 agtgaccgac aaaaatcccc agaataccga tatggaacag gttcgacgcc agattcatcc
    12541 ctttgcgatc cagcatctgg ctggacgccg cacgccatgt gtactgcccg tagtcataac
    12601 gcagccagct accaatcagg aagaccgccc cggctatgta cgggtagata tcaaagaaga
    12661 acatattcag gaattgcatt agtgctgtcc tccggtggtg atattcagat attgcggcgc
    12721 gacggcaccg gcaaagcgac gctgatgagc agtgattgct gaatcaccgc agcctttttc
    12781 agcaaagaat ttaacctgct cttcttccca gactgcatcc agcgcctgcg gcgtatcgtc
    12841 gcgcgcttca tcggcaattt tttccgccac tttgtcgctg tcgattgcgg tattcgccag
    12901 tttcagcagc agatcaaaca gcacggcata acggctttca cgctgttgca gacgcgcgct
    12961 cagcaatgcc agaatcggcg caatatcttt caaaccctcc acggcttcgc tttgcggcag
    13021 ctgcgccagg tactccagat acagcggcag atggtcgggc aattcgcggc tgtttaactg
    13081 caagccgtgc tgctcgtact gcgccagcag atccaccatc gcctggccgc ggtcacggga
    13141 ttcaccgtga acatgttcaa atagcaacag tgacgtggcg cggccacggt cgaacagttc
    13201 gctgtactgc gcctgggcat cgagcggatc catcgtcgtt aaatcgcgca ggaaaatgcc
    13261 cagcgcatgg gcatcctctt ttggcagatt tttcgacgcg gcaatcgcct caaacatctc
    13321 ttgttgatgc tgccataagg cagcatccgg atattcaagg agacgcgata caatcacgag
    13381 ttcgatcatg ggtgcggctc cgttttgctg gttacatcga tggcatcgat acggcggctg
    13441 ttgaacagat tgaatttggt atctgaaccg tggcagccat caccaaaggt aaagccgcag
    13501 ccatttttct ccgggaaggc ttcccgcgcc agttcacgat gactgctcgg caccacaaag
    13561 cgatcttcgt agttggcaat agccagataa cgatacatct cctgtgcctg ggcttcggtc
    13621 agaccgacct cttccagcgc acgggtatcg actttaccgt caacggtttc agcacgtttg
    13681 taatgacgca tcgccagcat acgtttcagc gcgcgcagta ccggtttggt atcaccggcg
    13741 gtcagcagat tcgccagata ctgtaccgga atacgcaggc tttcgacgtc tggcagaatg
    13801 ccgttgctgc ccagttcacc cgcgtctgcc gcagactgaa tcggcgacag aggcggcacg
    13861 taccagacca tcggcagtgt gcgatattcc ggatgcagcg gcagcgccag tttccattcc
    13921 atcgccattt tataaactgg cgactgctgc gcagcctcaa taacgctcag cggaataccg
    13981 tctttaatcg cctgctcgat gactttcgga tcgttcggat cgaggaacac atccagctga
    14041 cgctggtaga gatctttctc gttctcggta cttgccgcac gttcaatcgc gtcggcatcg
    14101 tacagcagca cgccaagata acggatacga ccgacacagg tttctgaaca tattgtcggc
    14161 tggccggctt caatacgcgg atagcagaag atgcacttct cagacttacc gctcttccag
    14221 ttgaagtaga tttttttgta cgggcatccg gtgatgcaca tacgccagcc acggcattta
    14281 tcctggtcga tcaggacgat gccatcttct tcacgcttgt aaatcgcacc gctcgggcag
    14341 gtcgccacac atgccgggtt cagacagtgt tcgcacaggc gcggcaaata catcatgaag
    14401 gtgttttcga actggctata catcgccttc tggatgttgt cgaagttctt gtctttcgcc
    14461 agcttgtcaa actcaccgcc caggtcatct tcccagttcg gccctttttc gattttcgcc
    14521 atccgttcgc cggtaatcag tgaacgcgga cgggcaatcg gctgtgattt gctgccttcc
    14581 ggcgcggtat gcaggttctg ataatcaaaa tcgaacggct cgtaataatc gtcgatcccc
    14641 ggcagatgcg ggttagcgaa gattttaccc agcagcatgg cacggttacc catgcgcggc
    14701 tgcagtttgc cgttgatttt acggatccag ccgcctttgt atttttcctg gttttcccag
    14761 tcagtcggga agccctggcc cggcttggtt tccacgttgt tgaaccacgc gtattccacg
    14821 ccttcacggc tggtccagac gtttttacag gtcactgaac aggtgtggca gccgatgcac
    14881 ttatcgagat tcagcaccat gccgacttgt gaacgaattt tcattttacg ctctcctgta
    14941 cctggtcatt gccttcgcca tctaaccagt caatgttctt catcttacgc actacaacaa
    15001 actcatcgcg gttagaacct acggtgccgt agtagttaaa gccatatgcc agatgggcat
    15061 agccgccgat catatgcgtc ggtttcggcg tgatacgggt gaccgagtta tggataccac
    15121 cacgctgttg ggtaatttcc gaaccaggca ggttaacgat acgttcctgc gcgtggtaca
    15181 tcatggtcat ccctgccgga acacgctggc tgacaaccgc acgggcagtc agtgcaccgt
    15241 tgctgttgaa gacttcaatc cagtcgttat cggcgatacc cagctctttg gcatcggctt
    15301 cactcagcca gaccaccgga ccaccgcgac ctaaagtcag catcagcagg ttgtcgctgt
    15361 aggtggagtg gataccccac ttctggtgtg gcgtcaggaa gttgagcgct ttttccgggt
    15421 tgccattgga tttctggccc atcacttctt tcaccgaacg ggtgtcgatc ggcggacgat
    15481 aaaccagcag gctttcaccg aaatcacgca tccattggtg atcctgatac agctgctgac
    15541 ggccagagag cgtacgccac gggatcagct cgtgaacgtt ggtgtaaccg gcgttgtaag
    15601 aaacgtgttc atcttcaagg ccagaccatg tcgggctgga gatgattttg cgcggctgtg
    15661 cctgaatatc gcggaagcga atcttctcgt cttctttatt cagcgccaga tgcgtatggt
    15721 cacggccggt aaactcgctc agggcagccc aggcttttac agctacctga ccattggttt
    15781 ccggtgccag cgtcaggatc atctctgccg catcaattgc ggtattgagc atcggctggc
    15841 cttttgccgg accttccgct ttggtgtagt tgagcttacg caggagatcc atctcgctct
    15901 gggtgttcca ggcaatccct ttaccgccgt taccgatttt ctccatcagc gggccgatag
    15961 aggtaaagcg ttcgtaagtt gctggataat cgcgctctac gaccataatg tgcggtgcag
    16021 ttttacctgg gatcaggtcg cattcgcctt ttttccagtc tttcacatcc agcggttgcg
    16081 ccagttcagc ggcagagtcg tgctggatag gcagcgtgac gatgtcagtt tctttaccca
    16141 gatgaccaac gcacacttcg gagaatttct tcgcgatggc tttgtagatt tcccagtcgc
    16201 ttttcgcttc ccaggccgga tcgaccgccg cagacagcgg gtgaataaac ggatgcatat
    16261 ccgaagtatt catgtcgtct ttttcgtacc aggtcgccgt cggcaaaatg atgtcggaat
    16321 agagacaggt gctcgacaga cggaagtcca gcgtcaccac cagatccagc ttgccttcca
    16381 gaccgttgtc ctgccagtcc acttcttccg gcttcacgcc gccctgctga cccaggtctt
    16441 tgccctggat accgtgctcc gtccccagca ggtacttgag cataaactca tgacctttac
    16501 cggaagaacc gagcaggtta gaacgccaga tgaacaggtt acgcgggtgg tttttaccgt
    16561 tttccggctg ttccgcagcg aaacggatag aaccctcttt cagggatttc accgtatagt
    16621 ccaccggatt catcccggct ttttctgctt cgcgagcgat agtcagcggg ttagtgccta
    16681 actgcggcgc agacggcagc cagcccatgc gttctgcacg gacgttaaag tcgatcaagt
    16741 gtccggtata gcgagattta tccgccatcg gcgacagcag ttcttccgca gtcacggttt
    16801 cataacgcca ctggctggag tggttataga aataagaggt actgttcatg tgacgcgccg
    16861 gacgctgcca gtcgagggca aacgccagcg gctgccagcc ggtttgcgga cgcagttttt
    16921 cctgacctac atagtgcgcc cagccaccgc cgctctgacc gacacagccg cagaaaatca
    16981 gcatgttgat cagaccacga tagttcatgt ccaggtgata ccagtggttc agcccagcgc
    17041 cgacgataat catcgaacga ccgtgcgttt tatcagcgtt atcggcaaat tcacgggcga
    17101 tgcgaataat ctggctgcga gaaacgccgg taatctgctc tgcccaggca ggggtgtaag
    17161 ctttcacatc gtcatagctg gttgcacagt taacgtcgtt caggccacgt tccagaccgt
    17221 agtttgccag cgtcagatca taaacggtgg tcaccagggc ggtgctgcca tcagccagtt
    17281 gcaggcgttt caccggcagc ttatgcagca gcacgttttc cagttccact ttgttgaagt
    17341 gttccgtgcc atcgccacca aagtacggga agcccacctc agcgatctca tcctggctac
    17401 ccagcaggct cagttgcagc tcggtttctt cgccagtttt gccgtcgcgc tgctcaagat
    17461 tccatttgcc cttctcgccc cagcggaagc caatagaacc gttcggcgca accatttcac
    17521 cattggtatt aaaggcgaca gttttccatt ccggattgtt ttcctggccc agtgcatcca
    17581 ccagatcagc agcgcgcagc atacgacccg cagcgtagta accgtcgcgc tcttccagca
    17641 tcaccagcat cggcatgtcg gtgtagcggc gcacatagtc ggtgaaatac tggcttgggt
    17701 tgtcgaggtg gaattcacgc agcattacgt gacccattgc cagcgccatt gccgcatcgg
    17761 tgccctgttt cggtgccagc cacagatcgc acagtttggc gatttcagcg tagtctggtg
    17821 tgacggcaac agttttggtc cctttgtaac gcacttcagt aaagaagtga gcatccgggg
    17881 tacgcgtctg cggcacgttt gaaccccagg cgatgatgta gctggagttg taccagtcag
    17941 cagattccgg tacgtcagtt tgctcacccc aggtttgcgg agacgcagga ggcaggtcgc
    18001 agtaccagtc gtagaagctt aagcaagtac cgccaatcag cgagagatag cgcgcacccg
    18061 atgcgtaaga aaccatcgac attgccggaa ttggcgagaa gccagcgaca cggtccgggc
    18121 cgtaattttt gatggtgtaa acgttagatg cggcgatcag ttcgttcact tcctgccagg
    18181 aagaacgaac aaaaccaccg cgtccacgcg cctgcttaaa gcttttcgct ttatcggcgt
    18241 cttcaatgat agaagcccat gcctcaaccg gatcgctatg cagcgccttc gcttcacgcc
    18301 acattttcat caggcgtttg cgcatcatcg ggtatttcag gcggttagcg ctgtaaagat
    18361 accaggagta gctggcaccg cgcgggcagc cgcgaggttc atggtttggc aggtccgggc
    18421 gagtacgcgg atagtcagtc tgctgggttt cccaggtgac cagaccgttt ttgacgtaga
    18481 ttttccagct gcaggagccg gtgcagttta ccccgtgggt agagcggacg attttgtcat
    18541 gctgccaacg ctggcgatat ccatcctccc agtcacggtt ggtattgaga agctggccat
    18601 gcccatcggc aaaggtttca cccttctgtt tgaagtagcg aaaccggtcc aggaatttac
    18661 tcatcggttt tctcctgtgg gagcctgtcg gccctctgat aaatcgacat tgctaatgtt
    18721 taagggcaag gtaacgctct gaaacagcgt gggaattgat aacgatcaag agtgatgggg
    18781 atgaaaaata aagtaaattc ctggaatacc cctttaggat aattaacatt tatagcataa
    18841 caga
//
LOCUS       DAZFJL010000090        18804 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFJL010000090 DAZFJL010000000
VERSION     DAZFJL010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.090"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(14..928)
                     /gene="lafA"
                     /locus_tag="ACHJ8C_003112"
     CDS             complement(14..928)
                     /gene="lafA"
                     /locus_tag="ACHJ8C_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HFO5552922.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
     gene            1414..2256
                     /locus_tag="ACHJ8C_003113"
     CDS             1414..2256
                     /locus_tag="ACHJ8C_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFO5552923.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLETLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDGKQQNIIKTIPKKGYLCNKEY
                     VSFPESSPAKKLIITDQIQKTTPEEISSTTPPVPVRKKHKGLMGLALAAAVILVGSTV
                     GYSHLKSTPDAPQLVKESINSPRIKIFHLSSGKENNSVPLLSQILAPGKDKLDNLLSA
                     HNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLNT
                     VQKC"
     gene            complement(2315..3292)
                     /locus_tag="ACHJ8C_003114"
     CDS             complement(2315..3292)
                     /locus_tag="ACHJ8C_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HFO5552924.1"
                     /translation="MQVGLTSSSLATGGAHSAAVSSSTVASTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQSTALMQML
                     DKRAALSGGAVDRQLQPVLQGEARVTFHSPDLANLVHNPTPGTRMFSVSDGRQTQLSA
                     VMLSEDDSAGQYQTRLTNALRRVGVQVHQQADGISFSTTEKQWPDIERTLSVRTDGDK
                     SAFMPLKTFAEPSQAERLAQSLQQGGAGISQTLEGINQQRAQMAVQQEKARQLIDGMS
                     RFPQTENAVQASENLGGVLDSANHNYQVLLQAVNGQARISSQTVRNLLG"
     gene            complement(3309..4238)
                     /gene="flgL"
                     /locus_tag="ACHJ8C_003115"
     CDS             complement(3309..4238)
                     /gene="flgL"
                     /locus_tag="ACHJ8C_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO5552925.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGDLAHVVEQMATGKEILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLAVRDDLLEAANGTN
                     TADSLASLGQDIESLTESMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFGSNLDVLNTLNSLSQELQNPDVDPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEATLKTYSKVSELNLFSVL"
     gene            complement(4253..5629)
                     /gene="flgK"
                     /locus_tag="ACHJ8C_003116"
     CDS             complement(4253..5629)
                     /gene="flgK"
                     /locus_tag="ACHJ8C_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO5552926.1"
                     /translation="MDMINIGYSGASTAQVELNVTAQNTANAMTTGYTRQVAEISTIG
                     ASGGSPNSAGNGVQVDSIRRVSNQYQVNQVWYAASDYGYYSTQQGYLTQLEAVLSDDN
                     SSLSGGFDNFFAALNEATTSPDDSALREQVISEAGALSLRIDNTLDYIDSQSTEIISQ
                     QQAMVSQINTLTSGIASYNQQIAQAEANGDNASALYDARDQMVEELSGMMDVQVNIDD
                     QGNYNVTLKNGQPLVSGQQSSTIALETNADGTASMTLTFAGTISTMTTDTGGSLGALF
                     DYQNDVLTPLTDTINSMASQFADAVNNQLAQGYDLNGNPGEPLFIYDASNADGPLTVN
                     PDITADELAFSSSPDESGNSDNLQALINISTEPLEIANLGSVTVGQACSSIISNIGIY
                     SQQNQTEVDAASNVYSAAQNQQSSVSGVSMDEEAVNLITYQQIYEANLKVISAGAEIF
                     DSVLEMCS"
     gene            complement(5818..6117)
                     /locus_tag="ACHJ8C_003117"
     CDS             complement(5818..6117)
                     /locus_tag="ACHJ8C_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HFO5552927.1"
                     /translation="MKVNASGGIDGSDALMGPKVQANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMIVKQLSPKHK"
     gene            complement(6117..7217)
                     /locus_tag="ACHJ8C_003118"
     CDS             complement(6117..7217)
                     /locus_tag="ACHJ8C_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO5552928.1"
                     /translation="MQNWIKAAVIAVSLALPGVALAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMNATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTSVSVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVALHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHVRPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            complement(7232..7897)
                     /gene="flgH"
                     /locus_tag="ACHJ8C_003119"
     CDS             complement(7232..7897)
                     /gene="flgH"
                     /locus_tag="ACHJ8C_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO5552929.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPTTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FGHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADARISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            complement(7980..8765)
                     /gene="flgG"
                     /locus_tag="ACHJ8C_003120"
     CDS             complement(7980..8765)
                     /gene="flgG"
                     /locus_tag="ACHJ8C_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO5552930.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTQEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            complement(8844..9581)
                     /locus_tag="ACHJ8C_003121"
     CDS             complement(8844..9581)
                     /locus_tag="ACHJ8C_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HFO5552931.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGSGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLAKNPEGMLVTADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            complement(9581..10783)
                     /gene="flgE"
                     /locus_tag="ACHJ8C_003122"
     CDS             complement(9581..10783)
                     /gene="flgE"
                     /locus_tag="ACHJ8C_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO5552932.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVTCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFDASD
                     AAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPTTPQTIEFQTDAAAPIDLTVDYSTCTQYGSEFSVTTN
                     AADGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            complement(11046..11759)
                     /locus_tag="ACHJ8C_003123"
     CDS             complement(11046..11759)
                     /locus_tag="ACHJ8C_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HFO5552933.1"
                     /translation="MNTLALNAQSPQPTATEESNNVAASNTTSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPEGSSSVGPGQV
                     DFSINPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDVA"
     gene            complement(11759..12190)
                     /gene="flgC"
                     /locus_tag="ACHJ8C_003124"
     CDS             complement(11759..12190)
                     /gene="flgC"
                     /locus_tag="ACHJ8C_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO5552934.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            complement(12193..12528)
                     /locus_tag="ACHJ8C_003125"
     CDS             complement(12193..12528)
                     /locus_tag="ACHJ8C_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HFO5552935.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            12610..13347
                     /gene="flgA"
                     /locus_tag="ACHJ8C_003126"
     CDS             12610..13347
                     /gene="flgA"
                     /locus_tag="ACHJ8C_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO5552936.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRRWHDYRYTFKVYIPSQIATAAPCTKTPGVTLTSPGDIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMAKSLVARDTPLTANDVELKPYNVSAQRREVLMEPNDAI
                     GFSSKHALQPGRPITKEELISPVLVERDQPVMIVYQSAGITASMPGVALKNGRKGEMV
                     KVRNASSQRVISAMVAESGVVTTVSAE"
     gene            13428..13706
                     /gene="flgM"
                     /locus_tag="ACHJ8C_003127"
     CDS             13428..13706
                     /gene="flgM"
                     /locus_tag="ACHJ8C_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO5552937.1"
                     /translation="MKITPTMPGNHPTATMAGSSQPRKSTATTATTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSDKVAQMQAMLDSGSLQVDTEQLASDMLSFFQN"
     gene            13719..14147
                     /gene="flgN"
                     /locus_tag="ACHJ8C_003128"
     CDS             13719..14147
                     /gene="flgN"
                     /locus_tag="ACHJ8C_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HFO5552938.1"
                     /translation="MSIRLQQVKALLQGIRDDDALYDSLRELLQRQRICMIRRASEDL
                     LAVNDEITQHYEQLHGHSYQRHSLLKMLGVSVNRDGLAQVFAWLPAVQKAAAQQLWQR
                     LEQKAERCKAYNDKNGELLIRQYEFIQAFLGSEADFLYQG"
     gene            14275..15207
                     /locus_tag="ACHJ8C_003129"
     CDS             14275..15207
                     /locus_tag="ACHJ8C_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HFO5552939.1"
                     /translation="MSFIECYEPEYVRNFMARHPDSPLSVRKVWKNEIRLSLMLTEPE
                     SCEAVLNDACAFDLQLTYRPVEENAAELSARDAIVNQVILSTLTLPDLTPELSLYAIG
                     IVLSRASKMPGRDGDILARLTTLPQALADHAQKGTLQAQFAQLPPVPQLARQLMTLLG
                     SFTFDWSILPESPRKASLPLQVTLLTLHDANSKALLQQQLKVQWQTTWQQHFATAPWM
                     MRNWLIYRVYHEVIGQTDGADYCPLVCDFYLIRTLISLWTLDGSPLRQEDIFALFAMF
                     ERWRASENAVLIRQQIQAECSADPLLYAFSLLSC"
     gene            15254..17692
                     /locus_tag="ACHJ8C_003130"
     CDS             15254..17692
                     /locus_tag="ACHJ8C_003130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO5552940.1"
                     /translation="MQTLIKTLATARVKPFVSVVTPTWNRGAFLPYLLYMYRYQDYPA
                     DRRELIILDDSPQSHQHIIDRLTNGTPGSFNIRYIHHPEKLPLGKKRNMLNELARGEY
                     ILCMDDDDYYSPDKISYSIAMMQKHRALISGSDQIPIWYSHINRIFKSRSFGPHNILN
                     GTFCYHRNYLKKHRYDDDCNLGEEKSFTDNFSVNPLQLPGERTILCISHSHNTFDKDF
                     ILGASTPVNAALTDIVSDPLLRNAYLGLHNATHYQAIQHQAIDQIVLLNLDKRPDRLQ
                     QIREELTLLHIPPEKITRLAASENENGQRGRQQSHLQALHLAQQHGWQNYLLLEDDAV
                     ILKQEKHIQVLNALLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAYLV
                     NSRYYPQLAQQMNNDEHSLEDGWQPLLRTDKWLACYPSLCYQRPGFSDIEKKATDNIN
                     HYFNKLPVATKPSTLPIADTIGFFMETSFHYALYRPIITALQAQGQSCTLVINDRVFK
                     PFLDEMLATLKSIDDPQLKGMRLSEMQAHGQRVKCLVSPYHTPALSGISPVNVRAMYG
                     LAKETWNHADWNRFYQRILCYSHYSQQALAHFGSAKVVGNPRFDAWHNGTLDRALPEN
                     IQPDVRKPTILYAPTFGALSSLPHWAEKLGRLSGDVNLICKLHHGTCSRPEEAASLAL
                     ARRHLKQRTDSARHTLALLAKADYVLTDNSGFIFDAIHVDKRVILLDFPGMNDLLDGE
                     KSYSTAESADQRIREIFPVAHDMAELRYLLSEAFDWDAVQARLTKIRHHYCDAFMDGK
                     AGERAAMVIVEALG"
     gene            18228..18608
                     /locus_tag="ACHJ8C_003131"
     CDS             18228..18608
                     /locus_tag="ACHJ8C_003131"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5552941.1"
                     /translation="MGKDKVIEKKQWNGIPPEYTDRWREIYNNAAPGEGMHLSDACPV
                     CGHTELYRYYSLLKTQPRLLGGIMYKGPGSYWEWCGSYRSYEHMSGYVPISWVVELSG
                     IDHSKLTAIPDMIDIARRKNQYIS"
BASE COUNT         4177 a         5171 c         4980 g         4476 t
ORIGIN      
        1 tgtcggcccg aacttactgc agcaggctgg aaaccatgct ggacatgctg ttggactgtt
       61 tcagcatggt gataccggtc tgcatcagca cctgttgttt ggtcatattg gacgcttccg
      121 tcgcgtaatc ggtatccatg atgttgccga tagccacttc ggtattgtcc tgcatgctgg
      181 tcaggttgtt ggcggtatca ttcagacggt tgatagacgc acccagtttg gactggatct
      241 gcgaaacgtc atccatcgcg gtagaaatgc tggtgatcat ctggttagcc gagccagacg
      301 cagtcagttc agtaccgcca gaaacgcctg cggtgccagt ggtgtctgcc tgatccgcac
      361 taaaggagtt gctgatagca gaaaggtcag tcaccagcgt gttcagttgg cttgaaatat
      421 tcacggtcat ggtgtcggaa gattccgcgc ccacctggaa agttactgcg ctctggaaca
      481 gaccgtcagt accggtgttg ctggtgccgg aaacaccgaa caggttggtg ccgccgtaag
      541 tagtgttttg cagcatgtca gacatctgct ggcccagttc gtcgtattcg tcctgcatcg
      601 cctgcaggtc atcgtcgctg taggtgccgt tcgctgcctg agttgacagg tctttcatac
      661 gacccagcac gtcagacatt tcgtcaaaca tgctgtcggc ggtttgcagc atcgcggtgg
      721 catcattgat gttgctcagc gccacgccca taccgctgga ctgtgccgtc agacggttag
      781 caatttgttt gcctgcggcg tcatcggcag aagagttaat ccggttacct gtcgccaggc
      841 gttccataga agtagacaga gaagagctgc ttttgctgat tgcattaacg gcggccattg
      901 aggcgttatt ggtgttaata gataacatga tactgctcct tgggattaat cgttttcgtt
      961 tcgataccct gtaaaaacga cacctcggga gcaaacttaa aaccgccgtt aaaaaaattt
     1021 cactgcggtt tatctgatgc cacttttagc taaaaaaagc agagtttaaa ttcagataaa
     1081 aagattatta gcaatatctg ccacatggcc aggtatcaaa atatgtcttc aacaaatgct
     1141 tgatatagtt aagattgtcc tcaattaacg cgttttctca tcaatgtact gatgccaaaa
     1201 tttacattgc gttgcaaata gtacccattt ggctattgta cctgtcctgt catttcgtta
     1261 catttccacg ccatatagtg attaagaata atcctattac cataaaatat taattatcac
     1321 gcagaaaaat catcttctca gggacgtata agactgtgct ttatgcaaag cactatcagg
     1381 aaacatttat cccttgctaa ggtaaactca actatgagta ttattatcaa caactggcgg
     1441 atggacccgt cgcttaatgc cttaatacat tgcgaaacgg gtgaaacacg tcgtctcggc
     1501 gaataccatt ttattttgct ggaaacatta gcaaaaaatg ccgatgtggt tttatcgcga
     1561 tcttatttat gtgccgaagt atggaagaac cgtattgtcg gcggaaacag cctaccgacg
     1621 gcgatccatg cgttgcgcgt tgctatcgat gatgatggca aacaacagaa cattattaaa
     1681 acaattccga agaaaggtta cttatgcaat aaagagtatg tgtcttttcc ggagtcatcg
     1741 cctgcgaaaa aactgattat taccgaccag atacagaaaa caacgccaga agagatatct
     1801 tcgacaacac cgcctgtacc tgttagaaaa aaacataaag gactgatggg gctggcgttg
     1861 gccgcggctg ttatattggt tggctcaacc gtggggtatt cacacctgaa aagtacacct
     1921 gacgccccgc aactggtaaa agagtcaatt aacagtccaa gaataaaaat atttcatctt
     1981 agttctggaa aggaaaataa ttccgtaccg ttactgtcac aaatcctcgc accaggaaaa
     2041 gacaaactcg ataatttatt gtcagcgcat aatatgacaa tgacaaccta ttataaatat
     2101 gttcgtaacc gactggaaag cgatattgtt ctgcgtaacc agtgcaacgg cagctggcaa
     2161 ttaaccttta atgtggaagg ttggcagaac agcgatatta atagcgcgat gtatcagaat
     2221 ttagagaagt tgttaaatac tgtgcagaaa tgctgacaag tgccaatata tgccggatgc
     2281 gacgctaaac gcgtcttatc cggccaaccg ataattaccc caataaattc ctcaccgtct
     2341 ggctggaaat tcgcgcctga ccgttcaccg cctgcaataa aacctgatag ttatgattgg
     2401 cgctatccag cacgccgccg aggttctccg atgcctgcac cgcgttttcc gtttgcggga
     2461 agcgtgacat gccgtcgatc aactggcggg ctttttcctg ctgcaccgcc atctgcgcgc
     2521 gctgctgatt aatgccttct aacgtctggg aaatccccgc cccaccctgt tgcaaacttt
     2581 gcgctaaacg ctcggcctgc gacggttcgg caaaggtttt tagtggcata aacgccgact
     2641 tatcaccatc ggtgcgcacg cttaaggtgc gctcgatgtc aggccactgt ttttcggtgg
     2701 tggagaaact gatgccgtcc gcttgctgat gtacctgcac gcccacgcgg cgcagggcgt
     2761 tggtcaggcg cgtctggtac tgcccggcgc tgtcatcctc agacagcatc accgccgaaa
     2821 gctgggtctg ccgcccgtca gagacactga acatgcgcgt tcccggcgtg ggattgtgta
     2881 ccagattcgc cagatccggc gagtggaagg tcacccgcgc ttcgccctgc aacactggtt
     2941 gtaactggcg gtccaccgcg ccgccggaaa gcgcggcgcg tttgtccagc atctgcatca
     3001 gcgccgtact ctgcgcctgc ccgcccttac gctggctgtg acgatagtcg agcaactgct
     3061 gttccagttg cccgagatag tgatccgcct gctgcaacgt ggtgagctgg tcgttaagct
     3121 gtacgctgta acgctgcggg cgagtggtaa tcagcggcga ggccggatat tcactcgccg
     3181 tcgccgggag tttttgccgc accgcctgag tggacgcaac ggttgacgac gataccgcag
     3241 cgctatgtgc gccgccggtg gcgagtgatg aagatgtaag tccgacctgc atactgttcc
     3301 tgaaagagtt agagaacgct gaacaaattc aactcactca ctttcgagta agtcttgagg
     3361 gtagcttcca ttgccacttc cagtccggta aaggtgatcg acgctgggcc gtaatcgaga
     3421 tcctgcaatg aaccaatcaa ttcatcatta gatgtcgaga tatcggtctg tgcatcgctc
     3481 aacatcgaca tggtgttttg tgtctcgccg agcgacgcaa tcgaggcgtt gaggtcgtcc
     3541 gaggcggtgt ccaccacatc gacggcgttc tgaatatcgc tctgcacctg cggatctgcc
     3601 gggtcgacgt ccgggttttg cagttcctgc gacaaggaat taagcgtgtt aagcacgtct
     3661 aaattactgc cgaaaaaatc gctcgccgcc acgttggtgt ccacttccac accgttcgac
     3721 acggtagtct ggcgatggtc gctgttgccc tgatagctat agctgtcggt gacgccatca
     3781 ccgtcatcat ccacagccac aatcggcggc tgatcgttga tggtaccgcc aaacacgtaa
     3841 tgcccgtctt catcctgata gttcagcgcc gcgaccattg attcggtgag cgactcaata
     3901 tcctggccga ggcttgccag cgaatcggcg gtattggtgc cattcgccgc ttcaagcagg
     3961 tcgtcgcgta ccgccagcag gctgttgttg acgccatcaa gaatagactc ctgctggctt
     4021 aagcccgccg acgcggagtc gatattgctc tgatactgtt caatcgccga ttgctggcgg
     4081 tttaactggg tgatgcgcgt ggcggcgatc gggtcatccg acggctgcaa aatttccttg
     4141 ccggttgcca tctgctcgac cacatgcgcc agatcgccgg agagattatt aaaactttgc
     4201 gtcatcgaga cgtaggtttg ttgggtggtt actcgcattt tcgcgctccc gcttagctgc
     4261 acatttccag caccgaatcg aaaatctcgg ccccggcgga aatgactttc agattggctt
     4321 cataaatttg ttgataggtg atcaggttca ccgcctcttc gtccatgctg acgccgctga
     4381 cgctactctg ctggttttgc gctgcggaat agacattgga cgcggcatcg acttccgtct
     4441 gattttgctg actgtaaatg ccgatattgc tgatgattga cgagcacgcc tgcccgaccg
     4501 tcacactgcc gaggttggct atctccagcg gttcggtgga gatattgatc agcgcctgca
     4561 ggttgtcgct gttgccgctt tcgtccggtg aactggaaaa cgccagctca tcggcagtga
     4621 tatccgggtt aacggtcagc gggccatcgg cattgctggc gtcataaata aacagtggct
     4681 cgccggggtt accgttgaga tcgtagccct gcgccagctg gttgttgacc gcatcggcga
     4741 actgcgacgc catgctgttg atggtgtcgg tcagcggcgt cagcacatcg ttttgataat
     4801 caaacagtgc gcctaatgaa ccgccagtgt cggtggtcat cgtcgagatg gttccggcga
     4861 aggtcagcgt catacttgct gtaccatcgg cgttggtttc cagcgcgatg gtcgagcttt
     4921 gctgcccgct caccagcggc tgaccgtttt tcagggtgac gttgtagttg ccctggtcgt
     4981 caatattgac ctgcacgtcc atcattccgc tcagttcttc caccatctga tcgcgggcgt
     5041 cgtacagcgc ggaggcgtta tcgccgttgg cttcggcctg agcgatttgc tggttatagc
     5101 tggcgatgcc gctggtcagc gtattgattt gcgataccat cgcctgctgc tgactgatga
     5161 tttccgtgct ttgcgagtcg atgtaatcca gcgtgttgtc gatgcgcaag gacagcgccc
     5221 cggcttcgct gatcacctgc tcgcgcaggg cggaatcatc ggggctggtg gtcgcttcgt
     5281 taagggcagc gaaaaagtta tcgaagccgc cgctcagact gctgttatcg tcgctcagta
     5341 ccgcttccag ttgtgtgaga tacccttgtt gggtgctgta atagccgtaa tcgctggcgg
     5401 cataccatac ctgattaacc tgatactggt tggagacgcg gcgaatactg tcgacctgca
     5461 cgccgttacc ggcgctgttc ggcgaaccac cgctggcacc gatggtgctg atctccgcca
     5521 cctggcgggt gtagcccgtg gtcatggcgt tagcggtatt ttgcgccgtg acgttcagct
     5581 ccacctgcgc cgttgatgcg ccgctgtagc cgatgttaat catgtccatg ttgggtcctt
     5641 accggtaaat cgtgccggtt aaaagcggcg tgaagttggg ggttcttaag ttccgtaaac
     5701 cgggtgccaa atgccggatg cggcgtaaac gccttatccg gcctacatac gggcgactcg
     5761 taggcctgat aagacgcgac agcgtcgcat caggcatcgt gcaccgaacg ccgaagctca
     5821 tttatgcttc ggcgacaact gcttcacaat catcgccgcg atcccaatcc cgtgctgctg
     5881 cgccagggtg ttacacaact catcgtcata aaacccctgc atcatgcgca ccgaatcgct
     5941 gttaaacggg ttatctttcg aatcaaacaa ctcgttggtt ttacgcatct ctttcagcat
     6001 gttgcgtaaa aaaatcgcct caaactgctc cgcggcctgc ttaatatcat tggcctgaac
     6061 cttcggcccc attaacgcat cgctgccgtc aatcccgccg ctggcgttca ctttcatcag
     6121 atcacctcca ggtcagcatc cagcgcgccc gcttcatgca gcgcctgtaa aatcgacata
     6181 atgtcgtccg gcgtcgcgcc caaactgttc aacgcattga ccagcgtttt caggctactc
     6241 gactccggca aactcaccac gcccgggcgc acatgattca ccgcgatatt gctctgcggc
     6301 gttaccgccg tgcgcccgcc cgcaaaagca tttggctggc tgacgttgct ggtttcgtta
     6361 atcgagacgg tcaaactgcc gtgcgacacc gccgccgcgt gcagcgcaac gccatcgccc
     6421 atcaccaccg tgccggtacg cgagttaaac accacgcggg cgcggatttt ctctgcctgc
     6481 acctgtacat catccagttg cgacataaat gccacccgcg cgccggggct ggtgggcgcg
     6541 cgcacgctga cgctggtgga gctctgcgca gtagcaatgc cgccaaacga ggcgttaatc
     6601 gccgccgcaa tattgttggc atctttaaac gacggacgtt tcaggttgag ggtgatagtg
     6661 tcgcccatct gaaaatcgct ggggatctcg cgctctaccg ttgcgccgtt aggaattaac
     6721 ccggcggttg gcgtattaac cgttacgctc gaaccgctgg ccccggtggc attcatcccg
     6781 cccaccacca cgctgccctg cgccagcgca taaacttcgc catccgcgcc gtgcaactgg
     6841 gtcagcaaca aagtgccgcc acgaatactt ttggcatcgc caatcgacga aacagtgaca
     6901 tcaatggtct gcccgcgcga gtacatcggc ggcagcgtgg cgctcaccgc caccgcggcg
     6961 acgtttttca ccttcgggtc gattttattc ggcagttgca cgccaaactg gcgcagcatg
     7021 ttggtgatgg tctggttggt aaatttgacc tggtttttat caccggtgcc gtcgaggcct
     7081 accaccaggc tgtagcccac cagttggttt tcgcgcacac cctgtacgtt gaccagcgac
     7141 tccagcgatt gcgccagcgc cacgccaggc agcgccaggc tcacggcgat aactgcggcc
     7201 tttatccagt tttgcattgc actctccggc attaaatcgg gaacagagga tggttaaaca
     7261 accgcgtcag ccagcctgcg gcattggcgt cgcttaacgc gccgcgtccg gcataggaga
     7321 tgcgggcatc ggcgatccgc tgggaggaaa ccgagttgtc gttctggatg tcgtcggcgc
     7381 gcacaaggcc gcttaaacgg atgtactcat cgccctggtt gagggtcagc catttttcgc
     7441 cgcgaatttc caggatgccg ttgggctgca ccgcatggac ggaaacggtg atctccccgc
     7501 gcaggctgtt ctgctgctgg gaagtggcgc tgccgtcaaa gtcgcggttg gcgtcgatag
     7561 agccggaaag cttatctttg gtgtgtccaa aaatcgttgg cgcgccgata tcgacggtgt
     7621 tgcttttgcc gaagttggtt ttcgcctgtt tgctcgactg ggtggattct tcgaggatca
     7681 ccgtcaggat atcgcccacg cggtaagccc gccggtcggc ggttagcgac cagttgtagc
     7741 cggtttcaaa cacgcccccg gcacgcccgt ctgccggagg cggcgcttcg gtggttggtg
     7801 gcgcaaagta tgcgtcgtct tttttgacca gaatggcctg cgattcacac ccgctaagga
     7861 gtggaagcag cgccaccagc cagagataat ttttcacgtt ctgcctgttt taaatacagt
     7921 gagttatgtg ctcttgtagg tcggataaga cgcctcagcg tcgcatccga catttcgcct
     7981 tacagcgtct ggctgatgta ctgcaacata tcgtccgctg ctgacaccat tttggcgttc
     8041 atctcgtacg cgcgttgcac cgtaatcatc gccaccattt cgttgacgat atccacgttc
     8101 gagccttcga gcgcgttgtc ctgcaaggtg ccaagcccat cttcgcccgg cacgccttcg
     8161 gtcggctgac cgctggcggc ggtttccaga tagaggttgt cgccctcggc ggaaagcccc
     8221 gccgggttgg cgaagttcac cagcgtcagt tgccctaact cggtcacgtc gctgtcgccg
     8281 ggtaagatcg ccgtcaccgt accgtcctca ccaaacgcaa cattcgtcgc ccccgccgga
     8341 acgtcgattt ccggttgtaa cggcaaaccc tgcgagttag tcagcacgcc gtcggcgttc
     8401 acctgcaagt tgccatcgcg ggtataggcg atatcgccgt tagccttttg cacctgtaaa
     8461 aatccctgcc ccataatggc gacattcatc gcgttgtcgg tggtttcgac gttgccttcg
     8521 gtgaaggttt tctgagtgcc gacaatgcgt acgccgctac caaattgcag cccggtcggc
     8581 ataatgttgt tctgatcgag catcgcgccc ggcgcttcct gggtctggta aaacaggtcc
     8641 tgaaacatca cccggtcgcg cttaaagccg gtggtgttaa cgttggcgat gttgttggca
     8701 atcgcgctca tttcagcatc ctgcgccgag aggccggttt tgctgatcca tagtgctgca
     8761 ttcatggtgg taaattccgt gttatttttt ggccgcggtt gccggatgcg gcgtgaacgc
     8821 cttatccgac ctacgatgtg cggttacgag ccgcgcaata agcggttgcc cgcgtcgctg
     8881 atatcttcgg cggttttcat catcttgatc tgcgcttcga actggcggtt catggcgatg
     8941 gacgacatca tctcgctcac cgccgagacg ttgctgccct cgagaaaacc gccgctgact
     9001 ttgatgtttt catcgcgctg ggccggaacg ccgtcggcag tcaccagcat cccttcagga
     9061 tttttcgcca ggttcgcgac cgggatatcc accagcttca ggcggtcgat atccatcgtc
     9121 gcggtcacgt caccgtcatc gggcgtcacg gagagcgtgc cgtcgctgcc aaacgaagca
     9181 atggcgttgg gcggcagaat gatcggcccg ttatctccca gcaccagatt gccgtcgata
     9241 gtcagttgcc cctggtcatc ctgctgaatg ttgccgttgc gggtatacac ttcgttgccg
     9301 tttttatcct gcacagcaat gtaacctgaa ccctgaatcg ccacgtccag tgggcgttcg
     9361 gttttctccg ccacaccggt gctgtcgttc acgccgctgg ccccttcctg cgccatatag
     9421 cgcgtgtcga agccgccgcc tttcaccttg tcgttagtcg ccattgccat atcggcgcga
     9481 aaaccgttgg tgttgacgtt tgccaggttg ttggcgctga tctgctgctc gtacagggtc
     9541 tgagaagcac cgctcagggc ggtataaatc aaacgatcca ttacattgcc tggaacagcg
     9601 cgtcatcgag ctgcgtgctg gtggcgataa ctttggtgtt ggcctgatag ttacgctggg
     9661 cggtcatcag gttgactaac tcgctggtga tatcgacgtt agagctttcg agcacgcccg
     9721 acgacagcgt gccgcaggtg ccggaaccgg gaacgccaat caacggggtg ccggattcgc
     9781 cggtttgtac ccacgcggtg ccgctcaccg cttcgaggcc gttttcattc gggaacgtcg
     9841 ccagcaccac ctgcccctgc aacatgcgct cgccgttgct gtaggtagcg taaactttgc
     9901 catcgtcatc aacctgcaca ccgttctgag tggcagaggc gtagccgtcg gcggcgttgg
     9961 tggtgaccga aaattcagag ccgtattgcg tacaggtcga gtaatcgacg gttaaatcga
    10021 tgggtgcggc ggcgtcggtc tgaaactcaa tggtctgcgg cgtggttggc gaggtcagtt
    10081 tcccggtgtt cgggtcgaag gttaacgtcg tcgccggaac gccggtctgc tgctgaccgt
    10141 cgaaggtgta ctgcacttcc caggtgttat cgctggtttt ggtgaagtac tggcataccg
    10201 aatgttcgtt gcctaatgag tcgtataccg tggtggtgta gctgtcggtg tacgagctgc
    10261 tgttggtcgc gtcgaacggt acggtagtgc ggtcgatagc cgcatcgctg gcatcgaagt
    10321 tggcggtaaa ggtcaggctg ctgctcgcct gcgccggaat attaccggtt ttgatctgga
    10381 tatcggtgac tgtaccggtt tgcagggtgc ccgtgtcatc gaccggatag ccctgcaaat
    10441 aagcgcccga ggcgttgacg atatagccgt ttttgtcggt ttcaaacgat ccggcgcggg
    10501 tataagaaat gttgcccgcg ctgtcgcagg taacaaaaaa accgtcgtcg ttaatcgcca
    10561 gatccagcgc gttaccggtg gataccagcg aaccgccgcg cgaaatactt tgcgcggtgc
    10621 ctgccacgct gacgcccatc gcctgacttc cggcatacat ggctgaaaac tgggtggtca
    10681 tcgacttata gcctaccgtt ccggcgttgg cgatgttgtt actgatcccg tccagctgtt
    10741 cgttaacggc attcagcccc gttgcggcaa tttcataact cattgtttct tcctgttaat
    10801 tcagattttg gcagcgtgcc cgttgttttg ccggatgcgg cgtgaacgcc ttatccggcc
    10861 tacgaacggg tgcaaacata aaaatatgcg cgaactcgta ggcctgataa gacgcgttaa
    10921 gcgtcgcatc aggcatcttg caccgaacgc cggatgcggc gtgaacgcct tatccggcct
    10981 acaaaaccat gcagtttcat gtaggcctga taagcgaagc gcatcaggca atttggcacc
    11041 ggacatcacg ccacatcact gtcggcatcg ggtacgccaa actggctaat catggtgaac
    11101 ggtacttcgc ccacgcctgc aacgttaagc accggggagc tgccatcgag cgggatgcgc
    11161 acatcctcca cttcgccgga aacttcaatc ggcacctctt cttccccggt gttggtcacc
    11221 accgagacgt cgtaatcgcc cggcgggatg ccgtaatccg ccggattaat cgagaaatcg
    11281 acctggcctg gtccaacgga cgaactgcct tccgggatca gcgacaccgt atagtcatcg
    11341 cctgcggcat cggtaaagtg gagatcggcg gtggtacagg cgtcgtccag cgtggcgcga
    11401 ccgttaatgg tctggtcgct cacctgcaag gcggtggtct gcaccataat gtcgtcgcct
    11461 accagattgc cgagcgccat cacctgaatg ttgctcatca acaccgcgtt ggtattcgcc
    11521 tgcacgctca tctcttccat catcgccacc tgcgccattg acgaaagctg gtcgatatat
    11581 tcggtggcgt cggtcgggtc ggtcgggtcc tggttttgga tctcagcgac aaacagcgtc
    11641 aggaaagtat caacgggcga gctatcgctg ctgcttgtcg tgttgctggc cgccacgttg
    11701 ttgctttcct ccgtggcggt cggctgtggt gattgcgcat tcagcgccag ggtattcatc
    11761 aggcttctcc cagtttcagc aaactttgct gcatactttt cacgttgttg agcacatcga
    11821 cattggtttc gaaatcgcgc gaggccgaca tcatgtccgc catctgttcc accacgttga
    11881 cgtcgggata ccagacatcc ccgttagcat cggcgagcgg cgaatgtggt tcatagcgtt
    11941 tcaccgcccc gccggtttgc agcacgtcct gcacctgcac gctggcaccg tcaatggcat
    12001 ggcgatgcgt tcccgccagc aagctgtggt gatagaccgc cgcaaacacc gggctgcgcg
    12061 ctttatacgc ctgcgcctcg ctgctggcgg gtgcattggc attcgccagg ttactggcaa
    12121 cggtattcag acgtagcgtt tgcgccgtca tcgccgaacc ggaaatctga tagatatcag
    12181 taaatgacat gcttatttcc cttcaatggc ctctctgatg ccgctgattt gcagattcag
    12241 aaaggtcaga ctgctttgat aatccatact attttgtgaa aaccgcgcct gttcgctgtt
    12301 cagttccacg gtgttgccat cttccgacgg ctgcatcggc acacggtact ggacatccac
    12361 cgccgcgtta tttgcccgtt gcatctccct ggcaaaatca atatctttag ccttgaaatt
    12421 tggcgtatcg acgttcgcca gattcgcagt cagtagctcg gtcctctcaa gacgtaactt
    12481 cactgcctgc ggatgtactc ccaatgcctg ctgaaaactg atccccattt cctgcgtcac
    12541 tcctgcttca atccgcatac gctaagcaat cgccgtgcca ttttttaagt gactgaagag
    12601 aaagggttta tgagttttca attttgcagg cggaagtttt atttcctcct ggcgctggcc
    12661 ttaccgggct acgccgccgt ccatcccgtg cagcacagcg cgcgggaaca agtcaacgcc
    12721 caggtactga atgccgccag ccaaaaaatt gaatcgctgg cgcaacagcg gcgctggcat
    12781 gactatcgct acacctttaa ggtctacatt ccctcgcaga ttgcgaccgc cgcgccctgt
    12841 actaaaacac ccggcgttac gctgacttcg cctggggaca tcgccctcaa ccggatgaac
    12901 ttcaccgtca gttgcccaca aagctggcag atgaacgtag cggtgcgccc ggatgtactg
    12961 gtgccagtag taatggcgaa gtcactggtt gcccgcgata cgccgctcac cgcgaatgat
    13021 gtggagttga agccttacaa cgtcagtgcc cagcgccggg aggtgttgat ggaacccaat
    13081 gacgcaattg gtttcagcag taaacacgcg ctgcaaccgg gcaggccaat caccaaagaa
    13141 gaactgatat ctccggtgct ggtcgagcgc gatcaaccgg tgatgattgt ttaccagagc
    13201 gcggggatca ccgcctcaat gcccggcgta gcgctgaaaa atggtcgcaa aggggaaatg
    13261 gtgaaggtgc gtaacgccag cagccagcgg gtcatcagcg cgatggtggc ggaaagcggc
    13321 gtggtgacaa cggtaagcgc ggagtaattt gcgctggaga ttaaagtttt gccgcctgtt
    13381 aaccgcttta ccggagtaac ccacccaaat accgagacga ggttacgatg aaaatcaccc
    13441 cgactatgcc aggcaatcac ccgacagcca caatggcagg ctccagtcag ccgcgcaaaa
    13501 gcacggcgac caccgccacg acgctgagcg ccgacgacat tacccaggcc ggattgcagt
    13561 ctgctcagca aacgctgaac gaccagcaag agagcgatat cgacagcgat aaagtggcgc
    13621 aaatgcaggc gatgctggac tccggcagcc tgcaagtgga caccgaacag ttagccagcg
    13681 acatgctgag ttttttccag aactaagagg ccgatgacgt gagtatccgg ttacaacagg
    13741 tgaaggcgtt gttgcaggga attcgtgacg acgacgcact gtacgattcc ctgcgtgaac
    13801 ttctccagcg tcagcggatc tgcatgattc gccgcgccag cgaggatctg ctggcggtaa
    13861 acgacgagat aacgcagcac tatgagcagt tgcatgggca tagctatcag cggcatagcc
    13921 tgctgaaaat gctcggtgtt agcgtaaatc gtgacggtct ggcgcaggtg tttgcctggc
    13981 ttccggcggt acaaaaagcg gcagcacaac agttgtggca acgccttgag caaaaagcag
    14041 aacgctgcaa agcctataac gataaaaacg gcgagctgtt aattcgccag tatgaattta
    14101 ttcaggcttt tttaggcagc gaagcggatt ttctctatca gggataacga caattccttc
    14161 ccccatcgcg gaaacggatt ttccgcctaa tccccaatcc tttgtttttg attaaaaaaa
    14221 ttcccggcac gcttattgca catcctgcaa ggtaataaag actggaaact acctatgagt
    14281 tttattgaat gttacgaacc ggaatatgtg cgcaatttta tggcacgcca ccccgattcg
    14341 cctttatccg tccgcaaggt gtggaaaaac gagattcgcc tgagcctgat gctgacagag
    14401 ccagaaagct gcgaagcggt gctcaacgat gcctgcgcct ttgatttaca gctcacctac
    14461 cgcccggtgg aagaaaatgc cgcagaactc tccgcgcgcg atgcaatagt aaaccaggtg
    14521 attttgagta ccctcacgct ccccgattta accccagagc tttcactata tgccatcgga
    14581 attgtgctct cccgcgccag taaaatgccg ggcagagacg gagatattct tgcccgcttg
    14641 acgacgctgc cacaggcgct ggccgatcat gcacaaaaag gcacactaca ggcacagttc
    14701 gcccagctcc cgcccgtgcc acagctggcg cgccagttga tgacgctgct cgggagtttt
    14761 acgtttgact ggtcgattct accggaaagc ccacgcaagg cctcgctgcc gttgcaggtt
    14821 accttgctga cgctacatga cgccaacagc aaagccctgt tacaacagca gcttaaggtg
    14881 cagtggcaaa ccacctggca acagcatttt gccaccgcgc cgtggatgat gcgtaactgg
    14941 ctgatttacc gcgtttacca tgaggtgatt ggacagacag acggcgcaga ttattgcccg
    15001 ctggtgtgcg atttttatct tatccgcacg ttaatcagtt tatggacgct ggatggttct
    15061 ccgctgcgac aggaggatat tttcgccctc ttcgccatgt ttgaacgctg gcgagccagt
    15121 gaaaatgccg tgctgattcg ccaacaaatt caggctgaat gttccgcaga tcccctgctt
    15181 tatgcgtttt cactgctgtc ttgctaacgc tttctggccg gttttcccgg ccttatcgac
    15241 ttcgtaaact atcatgcaaa ccctcatcaa aacactcgcc accgcgcgcg taaaaccgtt
    15301 tgtcagcgtc gtcacgccaa cctggaaccg cggcgcattc ctgccgtacc tgctttatat
    15361 gtaccgctat caggattacc cggcagaccg ccgcgaactc atcatcctcg acgactcccc
    15421 gcaaagccat caacacatca tcgaccgcct gaccaatggc acgccgggat ccttcaacat
    15481 ccgctacatc catcacccgg aaaaactgcc gctgggcaaa aaacgcaata tgctcaacga
    15541 actggcgcgc ggcgaataca tcctctgcat ggacgatgac gactactact cgccagataa
    15601 aatctcttac tccatcgcca tgatgcaaaa acaccgggcg ctcatctccg gttccgacca
    15661 gatccccatc tggtacagcc acatcaaccg cattttcaaa agccgcagct ttggcccaca
    15721 taacatcctc aatggcacct tttgctatca ccgtaactac ctgaaaaagc accgttacga
    15781 cgacgattgt aatttaggcg aggagaaaag tttcaccgac aatttcagcg ttaacccgct
    15841 gcaactgccc ggcgagcgca cgattctgtg tatctcgcac agccacaaca cctttgataa
    15901 agactttatc ctcggggcca gcacgcctgt aaacgcggcg cttacggata tcgtcagcga
    15961 tccgctgcta cgcaacgctt atctgggtct gcataacgcc actcattatc aggcgatcca
    16021 gcatcaggcg atcgatcaga tcgttctgct gaatctggat aaacgcccgg atcgcttaca
    16081 gcagatccgt gaagaactga cattgctgca tattccgcct gagaagatca cccgccttgc
    16141 cgccagtgaa aatgaaaacg gtcaacgggg ccgccagcaa tcacacttgc aggcgctgca
    16201 tctggcgcaa cagcacggct ggcaaaacta tctgctgctg gaagatgacg cggttattct
    16261 caaacaggag aaacatattc aggtgctgaa cgcgctgctg gcgagcctgg cgaaaattcc
    16321 ctggcaggtg atgatcctcg gcggcgaaat ttcgcaggga acgatgctaa aaagtttgcc
    16381 tgggctggtg cacgcacggg actgccgcaa agtgtgcgct tatctggtga acagccgcta
    16441 ctacccgcag ctggcgcagc agatgaacaa cgatgagcat tcgctggaag acggctggca
    16501 gccgctgctg cgcaccgaca aatggctggc ctgttacccg agcctttgtt atcagcgacc
    16561 gggctttagt gatatcgaga aaaaagcgac tgataacatt aaccactact tcaataagtt
    16621 acccgtagcc accaagccat ctacattacc tatcgccgat accatcggct tttttatgga
    16681 aacctcattc cactacgcgc tctaccgccc aatcatcact gccctacagg cgcaaggaca
    16741 aagctgcacg ctggtgatca acgaccgcgt gtttaaaccg tttctggatg aaatgctggc
    16801 gacgctaaaa agcatcgacg atccacagct caaaggaatg cgcttatcgg aaatgcaggc
    16861 acacggtcag cgagtaaaat gcctggtcag cccctatcac acgcctgcgc tcagtggcat
    16921 ttcacccgtg aacgttcgcg ccatgtacgg cttagccaaa gagacgtgga accacgctga
    16981 ctggaaccgt ttttatcagc gcattttgtg ttacagccac tacagccagc aggcgctggc
    17041 gcatttcggt agcgcaaaag tggtcggcaa tccacgtttc gacgcctggc ataacggcac
    17101 gctcgatcgc gcgttgccgg aaaatatcca accagatgtt cgtaaaccta cgatactcta
    17161 tgccccaacg tttggcgcct taagctccct gccccactgg gcagaaaaat tgggccgctt
    17221 aagcggcgat gtgaacctga tttgcaaact gcaccacggc acctgctcgc gcccggaaga
    17281 agccgcatcg ctggccctgg cgcgcaggca tttaaaacag cgcaccgact ctgcccgcca
    17341 cacgctggcg ctactggcaa aggccgatta cgtgctcacc gataacagcg ggtttatttt
    17401 cgatgctatc cacgttgata aacgggtgat cctgctcgac tttccgggca tgaatgattt
    17461 gctcgacggt gagaaaagtt attccacggc ggagagcgct gaccagcgga ttcgcgaaat
    17521 atttccggtt gctcatgaca tggcagaact gcgttacttg ttgtcagagg cgtttgactg
    17581 ggatgcggtg caggcgcggc ttacgaagat tcgtcatcac tattgcgatg cgtttatgga
    17641 tggcaaggcg ggagaacggg cggcgatggt gattgtggag gcgctgggtt aaaccgcaga
    17701 agtgcctgat gcgcttcgct tatcaggcct acgcgctcct acaatagttt gaatttgtga
    17761 ggatttgtat gccggataag gcgtttacgc cgcatccggc ataaacagcg catttatcta
    17821 ccctgtgatt tcaaatacat ctctttatga ctggagagtc gataaaagtt gcacattttt
    17881 gatcatgagg gagagttgtc tttatgaatt ccgaaaagcc cccttcttcc taccttaacc
    17941 attctttttt acctgtattg gcagcaacat acctcacccg tgatttattt gtagtacttc
    18001 gcgggtatca actataactt catgcctgta aacgactaaa acgggagggc ggtagcgttt
    18061 ctgatgtgat atgaacccat tttgttaatc aattagttgc caggatgccc tgggccgcaa
    18121 aaatatagcc atgagataga ttttttgagg gaacccggag taaaggagtg ggaaacaaaa
    18181 tgatagctct gtttatattt cagataatgc attagaggat tggataaatg ggaaaagata
    18241 aagttataga gaaaaaacaa tggaatggga ttccacccga atacacggat agatggaggg
    18301 aaatttataa taacgctgct cccggagaag ggatgcattt atcagatgct tgtccagtat
    18361 gtggtcatac agaattatat cgttattatt ctctgttaaa aacgcaacca cgcttattag
    18421 gagggattat gtataaagga cctggaagtt attgggagtg gtgtgggtct tatcgttcct
    18481 atgaacatat gtcaggctat gtgccaatat cgtgggttgt ggagttatca ggaatagatc
    18541 attcaaaatt aacggccatt ccagatatga ttgatattgc aagacgtaaa aatcaataca
    18601 tctcttaaat attatctaat tagttttccc ggacaggcca agttggcgaa aatcgtgaaa
    18661 aatatcacaa tatccatcct gacttacacc tcttctggtg attaaaatca aacctatgga
    18721 atgacgaaaa agatggaaag gaaaagcata tcaagtaatc agtcaggaag atattcacaa
    18781 caatcacctg aatcagtaga tcga
//
LOCUS       DAZFJL010000091        18722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFJL010000091 DAZFJL010000000
VERSION     DAZFJL010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.091"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(88..1341)
                     /gene="avtA"
                     /locus_tag="ACHJ8C_003132"
     CDS             complement(88..1341)
                     /gene="avtA"
                     /locus_tag="ACHJ8C_003132"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO5552942.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQNYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(1519..3549)
                     /gene="malS"
                     /locus_tag="ACHJ8C_003133"
     CDS             complement(1519..3549)
                     /gene="malS"
                     /locus_tag="ACHJ8C_003133"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO5552943.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLMLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFAEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPNNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSKADLRTLVDSAHQRGIRIIFDVVMNHTGYATLADMQEYQFGALYL
                     SGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRYGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGVGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            3869..4693
                     /gene="bax"
                     /locus_tag="ACHJ8C_003134"
     CDS             3869..4693
                     /gene="bax"
                     /locus_tag="ACHJ8C_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO5552944.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(4801..5979)
                     /gene="xylR"
                     /locus_tag="ACHJ8C_003135"
     CDS             complement(4801..5979)
                     /gene="xylR"
                     /locus_tag="ACHJ8C_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO5552945.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(6057..7238)
                     /gene="xylH"
                     /locus_tag="ACHJ8C_003136"
     CDS             complement(6057..7238)
                     /gene="xylH"
                     /locus_tag="ACHJ8C_003136"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO5552946.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(7216..8757)
                     /gene="xylG"
                     /locus_tag="ACHJ8C_003137"
     CDS             complement(7216..8757)
                     /gene="xylG"
                     /locus_tag="ACHJ8C_003137"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO5552947.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALKKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(8835..9827)
                     /gene="xylF"
                     /locus_tag="ACHJ8C_003138"
     CDS             complement(8835..9827)
                     /gene="xylF"
                     /locus_tag="ACHJ8C_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5552948.1"
                     /translation="MKIKNTLLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            10193..11515
                     /gene="xylA"
                     /locus_tag="ACHJ8C_003139"
     CDS             10193..11515
                     /gene="xylA"
                     /locus_tag="ACHJ8C_003139"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO5552949.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            11587..13041
                     /gene="xylB"
                     /locus_tag="ACHJ8C_003140"
     CDS             11587..13041
                     /gene="xylB"
                     /locus_tag="ACHJ8C_003140"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO5552950.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVAAQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGVQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIATNPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            13210..13551
                     /gene="yiaB"
                     /locus_tag="ACHJ8C_003141"
     CDS             13210..13551
                     /gene="yiaB"
                     /locus_tag="ACHJ8C_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO5552951.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFIRVCQLLALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            13597..14034
                     /gene="yiaA"
                     /locus_tag="ACHJ8C_003142"
     CDS             13597..14034
                     /gene="yiaA"
                     /locus_tag="ACHJ8C_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO5552952.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(14075..15070)
                     /gene="wecH"
                     /locus_tag="ACHJ8C_003143"
     CDS             complement(14075..15070)
                     /gene="wecH"
                     /locus_tag="ACHJ8C_003143"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO5552953.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPITWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQSRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLYTRTICGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            15245..15544
                     /gene="ysaB"
                     /locus_tag="ACHJ8C_003144"
     CDS             15245..15544
                     /gene="ysaB"
                     /locus_tag="ACHJ8C_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO5552954.1"
                     /translation="MMMNAFFPAMALIVLVGCSTPPPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            15639..16550
                     /gene="glyQ"
                     /locus_tag="ACHJ8C_003145"
     CDS             15639..16550
                     /gene="glyQ"
                     /locus_tag="ACHJ8C_003145"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO5552955.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            16560..18629
                     /gene="glyS"
                     /locus_tag="ACHJ8C_003146"
     CDS             16560..18629
                     /gene="glyS"
                     /locus_tag="ACHJ8C_003146"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO5552956.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
BASE COUNT         4679 a         4579 c         4730 g         4734 t
ORIGIN      
        1 acgccttatc cggcctacaa attctaccaa attcaatata ttgcagaaat catgtaggcc
       61 tgataagcgt agcgcatcag gcaattttta gtgactttca gcccaggctc tttctatctc
      121 ttccgccaga atcttcacac ccgcctcaat tttctccggc tctggtacgt agttcatgcg
      181 catacattga tgcgtatgcg gccacggttt atccagtccc gggaagaagt tgtgccccgg
      241 caccatcagc acgccgcgtg ctttcaggcg ctggtagagc tgctcggtcg taatgggcaa
      301 atccttaaac catagccaga ggaaaatggc tccttccggt ttatgaatca ggcagcgatc
      361 ttccggtaaa tagcggcgaa tgatggcgat agtttcctga acacgctggt agtaaaacgg
      421 tttgatgact gtttcagaca ggcgcagcag atcgttacgc ttaatcattt cacacatcat
      481 cgccggacca ataccgccag gtgccaggct gataatgccg ttcatattgg tgatggcggt
      541 gatgattttt tcattggcga tgataatgcc gcagcgggag ccaggtagcc ccagcttgga
      601 aagactcatg cacagcacga tattcggatt ccatagcggg cgcgcttcgc tgaagatgat
      661 acccgggaac gggacgccat aagcgttatc aatcaccagc ggaatgccgt gttgattcgc
      721 cagcgcgtca agcttcagca actcttcgtc agtaatcaca ttgcctgttg gattcgtcgg
      781 ccgggagacg caaatcatcc cggtttcttc gccaatatgt agatgctcaa aatcgacgtg
      841 gtatttaaac tggccttccg gcagcagttc aatattcgga cgcgcagaga caaacagatc
      901 ttcttccagt ccggcgtcag cgtagccaat gtattccggt gccagcggga acagcacttt
      961 tttgacccga ccatcggcac ggcgtccggc aaacagatta aataagtaga aaaacgcgct
     1021 ctggctgccg tttgttagtg caatattctg tggttcgata tcccagccca acttctcgcg
     1081 cagcattccg gcaagcagtg tgagtagctc cgttttcccc tgtggaccgt cataattgca
     1141 cagtgcatca gtcgctttgc cactttccag catgtcggtc agtagcgtct ggaagtagtt
     1201 ctgcatttcc gggatctgcg ccggattacc gccgccgagc ataatcgcgc caggcgtgcg
     1261 taaaccgtcg ttcagatctt ccatcagcag cgtaatgccg gagtggcggg taaatttgtc
     1321 accaaaaagg gagaatgtca tagcgggata tctgtcgaac tctgtagcaa ggaaggtaac
     1381 aataacgcta catttgctgg ggtgcaaatc ggtctgttga agagtgagcg gggctttatc
     1441 ctgcaatgct gattagggct gacattttat cctggttggc ggcttcccga ggtactgacg
     1501 aacgggaagc cggaaaagtc actgttgccc agcccagatg accagcactt tatcgtcgcc
     1561 atgctcacga acaaagccgt agccctgctt cagcgaaagt gtcgtttgtt tgcccacgcc
     1621 aattgcggga tggcgggcgc ggaactggct gattttttgc cagtgcgcga cgctggcggc
     1681 agatttaccg ctaacatcct gccagttcat atccgaacgc gtaccttgca gcggatcaga
     1741 accggtagga ccgaacggac gcgaggattc atcgccataa aagatttgta ccgcgcctgg
     1801 cgctaataat aataactctg ctgctttgtc gcccccttca cggaacaggc gggtatcatg
     1861 cgacgagagg tagctcaaca cgttgaaatc ctgcaatttc tccgccattt gctgccaggt
     1921 cgtatccatc tgcgccagac aatcgactgc tttcgccgcc tgctcctgat aatcgaaatt
     1981 gatcatcgca tcgaagccgt agcgatagta gtcactttgc atcacgccgt ggccccaggc
     2041 ttcaccggtc atccagaaag gtttgtcatc taatgctttg tcggggttag cttttttcca
     2101 ttcgcgaagt gcagcgctgg cttcggtttt cagttgctgc caggcgggca actcaacatg
     2161 tttggcggta tcgacccgaa aaccatcaat cccatagtcg cggacccact gacttaacca
     2221 gtgggttaag taatcgcgag gggtaaagcc gtcgatgact ttagcgtggg tatccgtttt
     2281 gtttttatag aacactggca gaccagaagc ggtagttgat tcggttttga tatccggcaa
     2341 aaaggccagc gacatagtga gatcgtcgaa tccaggattg tcgtaatcgc cgatatccgt
     2401 tctgatccag ttttttcccc accatttatc ccagcctgtt ttgtcgctga aattaatgta
     2461 atcgttaaag ctatgccagg tttgcccggc ggcaggtttc cagtcgctcc agcgttcacc
     2521 cagcgttttt ttcagctcgt caccagaaag gtataacgcg ccaaactgat actcctgcat
     2581 atccgccagc gtggcatagc cggtgtggtt catcacgaca tcaaagataa tacgaatacc
     2641 gcgctgatgc gcgctatcaa ccagcgtccg tagatcggct ttactgccca tattggcatc
     2701 aagattcgtc cagtcctgtg tgtaataacc atgataggca taatgcggga aatcaccttt
     2761 tgtaccgccg ccgacccagc cgtgaatttg ctcaaatggg gcacttatcc ataaagcatt
     2821 gacgcccaac tgctggagat aatccagttt gttggtcagg ccgcgtaaat cgccgccgtg
     2881 aaaagtgcca atttccgcca taccgtcttt atgacgtccg taactctggt cattattggg
     2941 atcgccgttt tcgaaacgat ctgtcagcac aaagtaaacc gtggcgttat gccagtcgaa
     3001 aggggcggat gtgtcagttt ctgcccgttc cagcaggagt aaaccgttgc tggtggcagc
     3061 gggttgtaac gttatttgac cgttcttcac tatcgcaatt tgctggctgt aataatcccg
     3121 gacggcggct ccctccgcaa aagtggcgct gacatccagt gtgagcggta atccatccca
     3181 tttcgggcat tcacggacca ggcttgctac cggttcgacg gcgttctgga tggaaatcat
     3241 caatgttggc gtaccggagc gggtgtctag ttgcagcgta tattcgccgt ccctgaacaa
     3301 tcgccattga ggcggcgtgt tgctacaagg ttgcagggaa agcatctgat tgagttttat
     3361 cgcatccgca ggctgccagc actgttggtc aaaatttagc attagtggac gcgtaccttt
     3421 gggcaactgc gcgtggctga caaatgttcc cgttccctgt tcgctaaagg cagggaatcc
     3481 cggagaagtc cagctggcag caacggcgaa gccaggaagg agtgtcagaa aacaggcggc
     3541 gagtttcatg gcggatgagt ccttattgct gcgatttcag acagtttgcc agcgataagc
     3601 cagacaaaac tcatccctga gcggtaagtc cacaggatga gaagcaaggg tgagcgatcg
     3661 cgcgcaaaaa acggctgaat tttgcgataa accccacatt ttctgcgatt tagcgtcaat
     3721 ctgaatcgtt aactcgtgac atagtttcag atttggacta ttccttgggg tgctcttgac
     3781 agctattttt gatatgattt gagattcaac tctcaaattt gtaaaaaata taaggtgttg
     3841 gaatgattaa atccgaccag gagacctgat gattttgact cccatacgac gatatggggc
     3901 gatgattctt atgttactca ctctggtgtt ttcgagtgag gtgttagcga agacgcacac
     3961 aacaacagcg agtcaaaagt cccacttaac taaagctagt aataaacagg taagcagtaa
     4021 acaagagtat tctcgcaata gtgcaaagag tagttcactt cctgatttgc gaaaataccc
     4081 ttccggaaca ccaaggaaaa aggcgtttct ccggactgta atgccttaca ttaccagcca
     4141 aaatgcggcc attactgcgg aacgtaactg gctgatttca aaacagtatc agggccaatg
     4201 gtcacctgct gagcgtgcgc gtctgaaaga catcgccaaa cgctacaagg tgaagtggtc
     4261 tggtaatacg cgaaaaatcc cgtggaatac cttgcttgaa cgcgtagaca ttatccctac
     4321 cagtatggtg gcgacgatgg ctgcagcaga aagcggttgg ggaacgtcga agctggcgcg
     4381 caacaacaac aatcttttcg gcatgaaatg catgaaagga cgttgtacca atgcgccggg
     4441 taaagtgaaa gggtactcac agtttagttc tgtcaaagaa tcggtgagcg cctatgtcac
     4501 taacctcaat acgcacccgg cttactcttc gttccgtaaa tcgcgtgcac aactgcgtaa
     4561 agcggatcag gaagtgactg ccacagcgat gattcacaag ctgaagggct actcgaccaa
     4621 agggaagagt tataacaact acctgttcgc aatgtaccag gataaccaac ggttaatcgc
     4681 ggcgcatatg taattgcatt tcctcgagcc ttatccgact tgtcaggcgg ataaggcttt
     4741 tgctcgtatc aggtggttgt gctgagttac ctgatgcgac gccgataatt ctcatcatcg
     4801 ctacaacatg acctcgctat ttacatcgcg atactctttt ggcgttgtgt catatgcttt
     4861 tttaaaaaca gagtagaaat attgcagcga tggataaccg cacatttgcg atatctcgtt
     4921 aattgacaag gtagtagaaa tcagcaggct gcgcgctttc tccagctttt cggcatgaat
     4981 catggcgtga atggtttcgc ccacctcttc tttaaaacgc ttctcaagat tggagcgtga
     5041 tatgcccacc gcatccagta cctgatccac tttaatccct ttacaggcgt gattacgaat
     5101 gtaatgcatg gcctgaataa cggcgggatc ggtcagcgag cggtaatctg ttgagcgccg
     5161 ttcaatgacg cgaactggtg ggaccaaaat ccgctgtagc ggcatttctt ctttatctaa
     5221 taatcgatgc aacagttttg ccgcctgata gcccatttgc cgtgcgccct gagcgaccga
     5281 agaaagggcg acacgcgaca gatagcgggt cagttcttcg ttatcgatgc caatcacgca
     5341 taatttttcc ggtacgggaa tgtgtagatg ttcacatact tgcagaatat gccgcgcccg
     5401 ggcgtcagtg acggcaataa tcccggtttg cggtggcagc gtttgtaacc agtctgccag
     5461 gcggttttgc gcgtgttgcc agttctctgg cgcggtttct aacccctgat aaaccactcc
     5521 gcgatacttt tcttcggcga caagctgacg aaatgcatat tcgcgttcag tggcccaacg
     5581 tttaccgctt gattccggaa gaccataaaa agcaaagcgg ttaacgcctt tctcttttaa
     5641 atgcaaaaat gcgctttcaa ccagcgcata gttatcggtg gcaatgtaat gaacgggtgg
     5701 gtaactttcg gcaaggtgat acgagccacc aaccccaaca atggggacgt cgacatcagc
     5761 cagcgcttgc tcgatttgtt tgtcgtcgaa gtcggcaatg acgccatctc ctaaccagtc
     5821 cttgatttta tcaatgcggg cgcggaaatc ttcttcaatg aaaatatccc attccgattg
     5881 tgacgcctgt aaatattccc ctacgccttc tactacctgc cggtcatagg ctttattggc
     5941 attgaacagt aatgtgatgc ggtgacgttt agtaaacatg gttcttttcc tgctgaatca
     6001 tgcaaaaact caaaaccggt aatacgttac cggctttgag aaaattttta tcaaaatcaa
     6061 gaacggcgtt tggttgcgga gtccatccat actgccagca acagaatcgc acctttaacg
     6121 atatactgcc agaaggtcgg tacatccatc atactcatgc cgttatccag tgaagccatg
     6181 atgaatgccc ccattactgc tccagccacg ctcccgacgc cgcctgccag actggtgccg
     6241 ccaatcacac atgctgcaat tgcgtccagc tcggcgatat ttcccgcaga aggtgaacca
     6301 gcgccgagtc gagaactgag gattaatccg gcgatggcta ccattaatcc gttaatagcg
     6361 aacacggcaa gtttggtgcg ttcaacgtta atcccggaga gacgtgccgc ttccagattg
     6421 ccgccgatgg cataaatgcg tcgtccaaat gccgtccgcg ttgccataaa cattccgccg
     6481 agtaacagca acgtcagcag cagaacagga gtgggaacac cacggtaatc attcaacagc
     6541 cagattgcgc ctaatacgat gatagcggtt aaagcctggc gaccgactac tgcggtagag
     6601 gccggagact gcaaacccaa agcctgacgg cgcattcttc cgcgccattg ccagccaaca
     6661 aaagccatta agccaagcgc gccaatgatg aagccggtgc tggcaggtag atagctttgc
     6721 ccaatttgtg acatcgcggc gctggtgggg gaaacagtcg tgccgttggt gatgccaatg
     6781 agtatgccac gaaatgccaa catgcccgcg agggtgacaa taaatgaagg gactttgcgg
     6841 tacgcgaccc accatccgtt ccaggctccg agaagcagtc ccagaaccaa cgtcacaatg
     6901 atggtaagtg gcaaaggcca gcctagccag acgtcacaaa tcgccgcgac gccacctaac
     6961 agccccatca ttgagccgac ggaaaggtcg atttcagcag aaattatgac gaacaccatt
     7021 cctaccgcga ggatgccggt aatcgcggtt tggcgtaaca ggttggagac gttacgggcg
     7081 cttaagtagg caccatcggt ggtccaggta aagaacagca tgattgcgat gatagctgca
     7141 atcatcacga agacctgcaa attcagtgat ttcagcccgg aaaagccacc ggatgtcggt
     7201 acggctaatt tcacttcaga cggattgctt ttcgacatga tgttcgctcc tcaatgcggc
     7261 ttccatcacc tgctcctgag tcaggttatg atttatcagg ttggctttta gtttcccttc
     7321 atgcatcacc agtacacgat cgctaaggcc gagcacttca ggtaattcgg aagagatgac
     7381 aataacggca ataccctgct ggacgagttg gttaattaat ttgtagatct cgtatttcgc
     7441 gccaatatcg atacccctgg tgggttcatc aagaatgaga atgcgcgggt taagtaacag
     7501 acagcgagcg aggatcgctt tttgttgatt gccgccgctc aaacgtccaa tagcaaggtc
     7561 gggggacgac gttttaactt tgagttgctg gattgattcc agaatacatt tttgctctgc
     7621 cgcgtcatca agctggctaa tgccaccggt aaattttttg agtgcggcga gggtaatatt
     7681 tttaccaacc gccattaccg gaacgatgcc gtcgcgcttt ctgtcttcgg gtaccatcgc
     7741 aatcccctgg gcgatggctt gctgacagtt acgaatatct acctgtttgc cgtcaatata
     7801 aatttttcct tcccattgtc cgggccacac accaaacagg cactgaatag tctcggtacg
     7861 tccggcacca acgagtccgg cgatacccag tatttcgcca cgtttcaggg aaaacgagac
     7921 atcattaact cgtttaatat gacgattaac cggatgccat gccgtcagat gttcaatacg
     7981 taatatttca tctccggtgg tatgtggttc attagggtaa agcgcggtta actcccgccc
     8041 gaccatcatg gtgataatat cgtcttcact cattccagca gcatcacgcg tgccaatgtg
     8101 ctgaccgtcg cgaataacgc aaatcgtatc ggaaatcgct ttgacttcgt tgagtttgtg
     8161 cgaaatataa atacaggcga taccgtgctg ttgtagatcg cgaataatat ccagtaaaac
     8221 cgacgtttcc tgctcagtta atgaggctgt cggctcatcg agaattaaca aacgcacctg
     8281 tttattaagt gccttggcaa tttcaaccag ttgttgttgc ccaagtccta aatcgccaac
     8341 gcgggtatca ggtgaaatgg ataaactgac ctgtgcgagc agcttctgac agcgtagcgt
     8401 catcaggtca taatccataa tgccattatg ggttatttcg ttacccagga agatattttc
     8461 cagcacggtc agttctttca ccagcgccaa ttcctggtga ataatggcga tacctttgcg
     8521 ttcggtatcg cggatgtgac tcgcctgaat ctcttctccc gcaaaaataa tttcgccttc
     8581 gtaggaacca tggggataaa taccacacag cactttcatc agcgttgatt tacctgaccc
     8641 attttcccca cagagtgaga cgatttcgcc agcattcaac cgcaagctga cgttatcaat
     8701 cgccttcaca ctgccgaagg ttttggtaat gttcttcatt tcaagtagat aaggcataac
     8761 gactccacct aagccaatca attcacgcgg catggagaga aatcacgccc ccgctccgcg
     8821 ccggggcgta acgcttacag ctcgctctct ttgtggaatc cgtctttaac taccgtatct
     8881 ttgatgttgt ttttattcac atcgatcggt gtcagcaggc gggagggaac atctttcagg
     8941 ccattattca gtgtggtatc cgcttttggc tcctgaccat tgcccaactc aacggcaatt
     9001 tctgcggcag tatttgccaa caatgtaata ggtttataca ccgtcatagt ttgcgtaccg
     9061 gcagcaatac gtttaatacc tgcaagatcc gcatcctggc cggaaattgc tactttccct
     9121 gataaacctt gcgcgcttaa tgcctgaatt gcccctcctg cggtggcatc gtttgaggca
     9181 actacagcat caattttgtt attattggcg gttagcgcgt tttccataat tttcaatgcg
     9241 ttttccggta accagccatc aacccattgg tcaccaacga ctttaatttt tccggaatca
     9301 acataaggtt ttaatacttt catttgcccg gcgcggaaca gcttggcgtt gttatctacc
     9361 ggcgagccgc ccatcaggaa gtaattacct tgcggaacaa tatcgaccag ggcttttgcc
     9421 tgcagttcac cgactttttc attatcgaaa gaaatataaa aatcgatatc cgcatcgtta
     9481 atcatacggt cgtaagctaa tactttaata ccttcttgtt tggcttcttt tacaacgtta
     9541 cttaatacct gaccgttata aggaataatg acaagaacat cgacaccccg gttaatcatg
     9601 ttttcaatct gcgacatttg tgtttcttca ttgccatttg cagactgtac aaatactttc
     9661 gcgccgagag attctgcttt tttcacaaaa atatctcgat ctttttgcca gcgttcaaga
     9721 cggagatcat caatcgccat acctattttg acttctttgg cgtgtgccgc aacgttggta
     9781 agcagaagtg aggtgcaaag ggtgagtaga gtgttcttta ttttcatggt gtagggcctt
     9841 ctgtagttag aggacagctt taataagtaa caatcaccgc gataaacgta accaattttt
     9901 agcaactaaa caggggaaaa caattacaga tttttatctt tcgattacga tttttggttt
     9961 atttcttgat ttatgaccga gatcttactt ttgttgcgca attgtactta ttgcattttt
    10021 ctcttcgagg aattacccag tttcatcatc ccattttatt ttgcgagcga gcgcacactt
    10081 gtgaattatc tcaatagcag tgtgaaataa cataattgag caactgaaag ggagtgccca
    10141 atattacgac atcatccatc acacgcggca ttacctgatt atggagttca atatgcaagc
    10201 ctattttgac cagctcgatc gcgttcgtta tgaaggctca aaatcctcaa acccgttagc
    10261 attccgtcac tacaatcccg acgaactggt gttgggcaag cgtatggaag agcacttgcg
    10321 ttttgccgcc tgctactggc acaccttctg ctggaacggg gcggatatgt ttggtgtggg
    10381 ggcgtttaat cgtccgtggc agcagcctgg tgaggcactg gcgttggcga agcgtaaagc
    10441 agatgtcgca tttgagtttt tccacaagtt acatgtgcca ttttattgct tccacgatgt
    10501 ggatgtttcc cctgagggcg cgtcgttaaa agagtacatc aataattttg cgcaaatggt
    10561 tgatgtcctg gcaggcaagc aagaagagag cggcgtgaag ctgctgtggg gaactgctaa
    10621 ctgctttaca aaccctcgct atggcgcggg tgcggcgacg aacccagatc ctgaagtctt
    10681 cagttgggcg gcaacgcaag ttgttacagc gatggaagca acccataaat tgggcggtga
    10741 aaactatgtc ctgtggggtg gtcgtgaagg ttacgaaacg ctgttaaata ccgacttacg
    10801 ccaggagcgt gaacagctgg gccgctttat gcagatggtg gttgagcata aacataaaat
    10861 cggtttccag ggcacgttgc ttatcgaacc gaaaccgcaa gaaccgacca aacatcaata
    10921 tgattacgat gctgcgacgg tctatggctt cctgaaacag tttggtctgg aaaaagagat
    10981 taaactgaat attgaagcta accacgcgac gctggcaggt cactctttcc atcatgaaat
    11041 agccaccgcc attgcgcttg gcctgttcgg ttctgtcgac gccaaccgtg gcgatgcgca
    11101 actgggctgg gacaccgacc agttcccgaa cagtgtggaa gagaatgcgc tggtgatgta
    11161 tgaaattctc aaagcaggcg gtttcaccac cggtggtctg aacttcgatg ccaaagtacg
    11221 tcgtcaaagt actgataaat atgatctgtt ttacggtcat atcggcgcga tggatacgat
    11281 ggcgttggcg ctgaaaattg cagcgcgcat gattgaagat ggcgagctgg ataaacgcat
    11341 cgcgcagcgt tattccggct ggaatagcga attaggccag caaatcctga aaggccaaat
    11401 gtcactggca gatttagcca aatatgctca ggaacataat ttgtctccgg tgcatcagag
    11461 tggtcgccag gagcaactgg aaaatctggt aaatcattat ctgttcgaca aataacggct
    11521 aactgtgcag tccgttggcc cggttatcgg tagcgatacc gggcattttt ttaaggaacg
    11581 atcgatatgt atatcgggat agatcttggc acctcgggcg taaaagttat tttgctcaac
    11641 gagcagggtg aggtggttgc tgcgcaaacg gaaaagctga ccgtttcgcg cccgcatcca
    11701 ctctggtcgg aacaagaccc ggaacagtgg tggcaggcaa ctgatcgcgc aatgaaagct
    11761 ctgggcgttc agcattctct gcaagacgtt aaagcattag gtattgccgg acagatgcac
    11821 ggtgcaacct tactggatgc tcagcaacgg gtgttacgcc ctgccatttt gtggaacgac
    11881 gggcgctgtg cgcaagagtg cactttgctg gaagcgcgag ttccgcaatc gcgggtgatt
    11941 accggcaacc tgatgatgcc cggatttact gcgcctaaat tgctatgggt tcagcggcat
    12001 gagccggaga tattccgtca aatcgataaa gtattattac cgaaagatta cttgcgtctg
    12061 cgtatgacgg gggagtttgc cagcgatatg tctgacgcag ctggcactat gtggctggat
    12121 gtcgcaaagc gtgactggag tgacgtcatg ctgcaggctt gccacttatc tcgtgaccag
    12181 atgcccgcat tatacgaagg cagcgaaatt actggtgctt tgttacctga agttgcgaaa
    12241 gcgtggggta tggcgacggt gccagttgtc gcaggcggtg gcgataatgc agctggtgcg
    12301 gttggtgtgg gaatggttga tgctaatcag gcaatgttat cgctggggac gtcgggggtc
    12361 tattttgctg tcagcgaagg gttcttaagc aagccagaaa gcgccgtaca tagcttttgc
    12421 catgcgctac cgcaacgttg gcatttaatg tctgtaatgc tgagtgcggc gtcgtgtctg
    12481 gattgggccg cgaaattaac cggcctgagc aatgtcccag ctttaatcgc tgcagctcaa
    12541 caggctgatg aaagtgccga gccagtttgg tttctgcctt atctttccgg cgagcgtacg
    12601 ccacacaata atccccaggc gaagggggtt ttctttggtt tgactcatca acatggcccc
    12661 aatgaactgg cgcgagcagt gctggaaggc gtgggttacg cactggcaga tggcatggat
    12721 gtcgtacatg cctgcggcat taaaccgcaa agtgttacgc tgattggtgg cggggctcgt
    12781 agtgagtact ggcgtcagat gctggcggat atcagcggtc agcagctcga ttaccgcaca
    12841 gggggagatg tggggccagc actgggtgca gcaaggctgg cgcagattgc gacgaatcca
    12901 gagaaatcgc tcattgaatt gttgccacaa ctaccgttag aacagtcgca tctaccagat
    12961 gcgcagcgtt atgccgctta tcagccacga cgagaaacgt tccgtcgcct ctatcagcaa
    13021 cttctgccat taatggcgta aacgttatcc cctgcctgac cgggtggggg ataattcaca
    13081 tctatatatc tcagtaatta attaatattt agtatgaatt tattctgaaa atcatttgtt
    13141 aatggcattt ttcagttttg tctttcgttg gttactcgta atgtatcgct ggtagatatg
    13201 gagatcgtta tgaaaacctc aaagactgtg gcaaaactat tatttgttgt cggggcgctg
    13261 gtttatctgg ttgggctatg gatctcatgc ccattgttaa gtggcaaagg ctattttctt
    13321 ggcgtgttaa tgacagcaac ttttggcaat tatgcatatc ttcgcgcaga aaaactcggg
    13381 caactggatg atttttttat ccgcgtctgc cagttgctgg cgttaatcac tatcggtctc
    13441 ttgtttatcg gtgttttaaa cgcacctatc aatgcttatg aaatggtgat ttatcccatc
    13501 gccttttttg tctgcttgtt tggtcaaatg cgtttgtttc gctcggcatg agcaacataa
    13561 acctcttaca cattcaggaa tgaaaggaat actgtgatgg acaacaaaat atcaacctat
    13621 tcaccggcat ttagtattgt gtcatggata gctctcgttg gtggtatcgt tacctatctg
    13681 ttagggctat ggaatgcaga gatgcagtta aatgaaaaag gatattattt tgccgtactg
    13741 gtattaggac tgttttctgc agcgtcttat caaaagaccg ttcgggacaa gtatgaaggc
    13801 ataccgacca cctccattta ttatatgacc tgcctgactg tctttattat ctctgttgcg
    13861 ttactgatgg taggtctgtg gaatgcgacg ttattactca gcgagaaagg tttttatgga
    13921 ctggctttct tcttaagctt gtttggtgca gtagcggtgc agaagaatat tcgtgatgcc
    13981 ggaataaacc caccaaaaga aacacaggtt acccaggaag aatacagcga ataattcaca
    14041 taagcccggt tagtccaatg taaccgggct tttactaact cactaatctg tttctgtcga
    14101 ttcgttgtac cagcatagaa agtaacaaac tcgctgccaa cgtcgcgcaa aagatccaaa
    14161 taatatcgag tattggccag tttttaagct caatcccccg ggtgcgcagt gcatggataa
    14221 tcaatgcatg gaacccgtat atacctaacg aatggcggga gattaagcca agtccgcaaa
    14281 tggtacgcgt atacagcgtg tttttaacca gagtcaataa cgcgattgcg cagataaaaa
    14341 ccatcggccc acagtaaaga taccaggtat cggcaaaatt tccgcgccac tgtaattcat
    14401 ataatgtccc gcgagagata ataaaaaccc ccgtcgcaaa cagtgcggcg ctcacccacg
    14461 acagtgcttt atgctgtgtg tccatcatcc ctatagcgcg gcccaacatg ccataaagga
    14521 tgtagtaaaa agtatcgcca ttgatatata agttaattgg cagccattca aaaccgtcga
    14581 ttttctgcgg cacagtattt gggttagcga tgataccaat caccaccatt agcaccagca
    14641 acatttttcc gccgacgttc ttcacctgaa tcagcggtga aaccagataa atcactgcaa
    14701 ttgcgaagaa aaaccataaa tggtaaaaca ctggcttttg cagcaggttt ttcagcgcta
    14761 actccacatt gatggaggta aacagcgcaa tgtagagcag cgcgattgcg ctataaaaaa
    14821 acaggcataa gccgatacgc aagaaatggc gcgactgggc gctgcgttcg ccaaaaaaga
    14881 gatagccgga aatcatgaaa aatagcggca cgctgacacg agaggcagaa ttcagaacat
    14941 tggcgatatc ccaggtgatg gggctaacac tatgagcatt ggtcacatac caggtagtgg
    15001 tgtgaatcat caccaccatt aaacacgcta tccctcgcag gttatcaatc cagtaaattt
    15061 tgggctgcat ctgtgtctct gtatctggtt aaaaaaagtc tgaccgataa atcattcagt
    15121 tggcgcactg gaataatctg agttttatca ctacagctta tagaggctta aggaaattcg
    15181 taagatatca gccactatac cgatataaat aataagactc acctgcaaac cagacggtaa
    15241 tttaatgatg atgaacgctt tctttccagc aatggcgctt attgtgctag tgggttgttc
    15301 tacaccgcca cccgtgcaga aagcacaacg ggtaaaggtt gatcctctgc gttcgttgaa
    15361 tatggaagcg ttatgcaagg atcaggcggc aaaacgttat aacaccggcg agcaaaaaat
    15421 cgacgtcacc gccttcgaac agttccaggg aagctatgaa atgcgcggtt ataccttccg
    15481 gaaagagcag tttgtctgtt cttttgacgc agatggccat tttttgcatc tttccatgcg
    15541 ttaagccctg ctttttcccg tttcgtactg tatatcttcc atccagcggg tatactgatc
    15601 ccttccttta aatccacacg tatccagcac gaaataatat gcaaaagttt gataccagga
    15661 ccttccaggg cttgatcctg accttacagg attactgggc tcgccagggc tgcaccattg
    15721 ttcaaccatt ggacatggaa gtcggcgcgg gaacctctca cccaatgacc tgtctgcgcg
    15781 cgctggggcc agaaccgatg gcggctgctt atgttcagcc ttctcgtcgc ccgaccgatg
    15841 gtcgctacgg cgaaaacccc aaccgtttac agcactacta tcagttccag gtggtcatta
    15901 agccatcgcc ggacaatatt caggagctgt accttggttc tctgaaagag ctgggcatgg
    15961 acccgaccat ccacgacatc cgtttcgtgg aagataactg ggaaaacccg acgctgggtg
    16021 cctgggggct gggctgggaa gtgtggctga acggcatgga agtgacgcag ttcacttact
    16081 tccagcaggt tggtggtctg gagtgtaaac cggttaccgg cgagatcacc tatggtctgg
    16141 aacgtctggc aatgtacatt cagggcgtag acagcgttta cgacctggtc tggagcgacg
    16201 gcccgctggg taaaaccacc tacggcgacg tgttccatca gaacgaagtg gagcagtcca
    16261 cttacaactt cgaatacgcg gatgtggact tcctgttcac ctgctttgag cagtacgaga
    16321 aagaagcgca gcaactgctg gcgctggaaa atccgctgcc gctgccagcc tacgagcgta
    16381 ttctgaaagc cgcccacagc ttcaacctgc tggatgcgcg taaagccatc tccgtcaccg
    16441 agcgtcagcg ctatattctg cgcattcgca ccctgaccaa agcagtggca gaagcatact
    16501 acgcttcccg tgaagccctc ggcttcccga tgtgcaacaa agataagtaa gaggcggcta
    16561 tgtctgagaa aacttttctg gtggaaattg gcactgaaga gctgccacca aaagcactgc
    16621 gcagcctggc tgagtccttt gctgcgaact ttactgcgga gctggataac gctggcctcg
    16681 cacacggcac cgttcaatgg tttgctgctc cgcgtcgtct ggcgctgaaa gtcgctaacc
    16741 tggcggaggc gcaaccggat cgcgaaatag aaaaacgcgg cccggcaatt gcccaggcgt
    16801 tcgacgctga aggcaaaccg agcaaagcgg cagaaggttg ggcgcgtggt tgcggtatta
    16861 ccgttgacca ggctgagcgt ctgactaccg ataaaggcga atggctgctg tatcgcgccc
    16921 atgtgaaggg tgaaagcacc gaagcactgc tgccgaatat ggttgcgact tcgctggcga
    16981 aactgccgat cccgaaactg atgcgttggg gcgcaagcga cgtgcacttc gtgcgtccgg
    17041 ttcacaccgt gaccctgctg ctgggcgaca aagttattcc ggctaccatt ctgggtattc
    17101 agtccgatcg cgtgattcgc ggccaccgct ttatgggcga gccggaattc actatcgaca
    17161 atgccgatca gtatccggaa attctgcgtg agcgcgggaa agtcatcgcc gattacgaag
    17221 aacgtaaggc gaagattaaa gccgatgctg aagaagcagc acgtaagatt ggcggtaacg
    17281 ctgatttaag cgaaagcctg ctggaagaag tggcttcgct ggtggagtgg ccggtcgttc
    17341 tgaccgcgaa attcgaagag aaattccttg cggtgccgtc tgaagcactg gtttacacca
    17401 tgaaaggtga ccagaagtac ttcccggtgt atgcgaacga cggcaaactg ctgccgaact
    17461 ttatcttcgt tgccaacatc gaatcgaaag atccgcagca gattatctcc ggtaacgaga
    17521 aagtcgttcg tccacgtctg gcggatgccg agttcttctt caataccgac cgtaaaaaac
    17581 gtcttgaaga taacctgccg cgcttgcaaa ccgtgttgtt ccagcaacag ttggggacgc
    17641 tgcgcgacaa aactgaccgc attcaggcgc tggctggctg gattgctgag cagattggcg
    17701 ctgacgttaa ccacgctacc cgtgcgggcc tgctgtctaa gtgtgacctg atgaccaaca
    17761 tggtcttcga gttcaccgac acccagggcg ttatggggat gcactacgcg cgtcacgatg
    17821 gcgaagcgga agacgtagcc gttgcgctga acgaacagta tcagccgcgc tttgccggtg
    17881 atgacctgcc gtctaacccg gtagcctgtg cgctggcgat tgctgacaag atggataccc
    17941 tggcgggtat cttcggtatc ggccagcatc cgaaaggcga caaagacccg tttgcgctgc
    18001 gtcgtgccgc acttggcgtg ctgcgtatta tcgttgagaa gaacctcaac cttgatctgc
    18061 aaacgctgac cgaagaagca gtgcgtctgt atggcgataa gctgactaac gccaacgtgg
    18121 ttgatgatgt tatcgacttt atgctcggtc gcttccgcgc ctggtatcag gacgaaggtt
    18181 acaccgttga caccatccag gcggtactgg cgcgtcgtcc gactcgtccg gctgatttcg
    18241 atgcccgtat gaaagcggta tcgcacttcc gtaccctgga agcagctgca gcactggcgg
    18301 cggcgaacaa gcgtgtatct aacatcctgg cgaaatctga tgaagtgctg agcgatcgcg
    18361 tgaatgcctc tactctgaaa gagccggaag aaattaaact ggcgatgcag gttgtggtgc
    18421 tacgtgacaa gctggagccg tactttgcgg aaggtcgtta ccaggatgcg ctggtcgaac
    18481 tggctgagct gcgtgaaccg gtcgatgctt tcttcgataa agtgatggtc atggttgatg
    18541 acaaagaatt gcgtctcaac cgtctgacca tgctggagaa actgcgcgaa ctgttcctgc
    18601 gagttgcgga tatttcgctg ttacagtaat aatgccgtta ttaaatagcc tgccatctgg
    18661 caggcttttt ttatcgctaa ataatacagc aacctttaat aatcttctga tgaataaaga
    18721 tt
//
LOCUS       DAZFJL010000092        18700 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFJL010000092 DAZFJL010000000
VERSION     DAZFJL010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.092"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            248..940
                     /gene="yieP"
                     /locus_tag="ACHJ8C_003147"
     CDS             248..940
                     /gene="yieP"
                     /locus_tag="ACHJ8C_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO5552957.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
     gene            963..2390
                     /gene="mdtD"
                     /locus_tag="ACHJ8C_003148"
     CDS             963..2390
                     /gene="mdtD"
                     /locus_tag="ACHJ8C_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO5552958.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYAHKHMPNFTTARRRFDTTGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIAPRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNHVPSESE"
     gene            complement(2356..3348)
                     /gene="rbsR"
                     /locus_tag="ACHJ8C_003149"
     CDS             complement(2356..3348)
                     /gene="rbsR"
                     /locus_tag="ACHJ8C_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO5552959.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(3352..4281)
                     /gene="rbsK"
                     /locus_tag="ACHJ8C_003150"
     CDS             complement(3352..4281)
                     /gene="rbsK"
                     /locus_tag="ACHJ8C_003150"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO5552960.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPLSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQALHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKTLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            complement(4407..5297)
                     /gene="rbsB"
                     /locus_tag="ACHJ8C_003151"
     CDS             complement(4407..5297)
                     /gene="rbsB"
                     /locus_tag="ACHJ8C_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO5552961.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKVGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            complement(5322..6287)
                     /gene="rbsC"
                     /locus_tag="ACHJ8C_003152"
     CDS             complement(5322..6287)
                     /gene="rbsC"
                     /locus_tag="ACHJ8C_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO5552962.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            complement(6292..7797)
                     /gene="rbsA"
                     /locus_tag="ACHJ8C_003153"
     CDS             complement(6292..7797)
                     /gene="rbsA"
                     /locus_tag="ACHJ8C_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO5552963.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            complement(7805..8224)
                     /gene="rbsD"
                     /locus_tag="ACHJ8C_003154"
     CDS             complement(7805..8224)
                     /gene="rbsD"
                     /locus_tag="ACHJ8C_003154"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO5552964.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAVIIAEEIKQHNPQLHETLLTHLEQLQQHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            complement(8391..10259)
                     /gene="kup"
                     /locus_tag="ACHJ8C_003155"
     CDS             complement(8391..10259)
                     /gene="kup"
                     /locus_tag="ACHJ8C_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO5552965.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10482..11978
                     /gene="ravA"
                     /locus_tag="ACHJ8C_003156"
     CDS             10482..11978
                     /gene="ravA"
                     /locus_tag="ACHJ8C_003156"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO5552966.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGALVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQITDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            11972..13423
                     /gene="viaA"
                     /locus_tag="ACHJ8C_003157"
     CDS             11972..13423
                     /gene="viaA"
                     /locus_tag="ACHJ8C_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO5552967.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRNRLKDARVPQELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSAITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(13428..14420)
                     /gene="asnA"
                     /locus_tag="ACHJ8C_003158"
     CDS             complement(13428..14420)
                     /gene="asnA"
                     /locus_tag="ACHJ8C_003158"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO5552968.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            14572..15030
                     /gene="asnC"
                     /locus_tag="ACHJ8C_003159"
     CDS             14572..15030
                     /gene="asnC"
                     /locus_tag="ACHJ8C_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO5552969.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15120..15563
                     /gene="mioC"
                     /locus_tag="ACHJ8C_003160"
     CDS             15120..15563
                     /gene="mioC"
                     /locus_tag="ACHJ8C_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO5552970.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEETGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            15942..17831
                     /gene="mnmG"
                     /locus_tag="ACHJ8C_003161"
     CDS             15942..17831
                     /gene="mnmG"
                     /locus_tag="ACHJ8C_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO5552971.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARIGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVEKDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTSLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            17895..18518
                     /gene="rsmG"
                     /locus_tag="ACHJ8C_003162"
     CDS             17895..18518
                     /gene="rsmG"
                     /locus_tag="ACHJ8C_003162"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO5552972.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         4702 a         4866 c         4622 g         4510 t
ORIGIN      
        1 tgagattctc aggagaaccc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt
       61 ggtggcgcat tatagggagt tattccggcc tgacaagagg aaatttaaaa taattttccg
      121 accgcgcatt ttttcagcaa aagccccgtt aatgtgccat aaaacaagca atttgccgtt
      181 tttgcaaccg taatcacact tcctggtggc atactaagga ggaataaaaa gaaaaggaag
      241 attagtaatg cctttaagcg cacaacagtt agctgcacag aaaaaccttt cgtatgttct
      301 ggctgagaag ctggcgcagc ggatcttaaa aggtgaatat gaacccggca ccattttgcc
      361 tggtgagatt gagctgggcg agcaatttgg agtgagtcgt acagcggtac gcgaagcggt
      421 caaaacgtta acggcaaaag ggatggtttt accgcgaccg cgaattggta cccgagtcat
      481 gccacaatcg aactggaatt ttcttgatca ggaattgctt acctggtgga tgacagaaga
      541 gaactttcat caggtcatcg atcactttct ggttatgcgt atctgtctgg agccgcaagc
      601 ttgcctactg gcagcaacgg ttggcaccgc ggaacagaag gcgcatctca atacgttaat
      661 ggccgaaatg gcggcattaa aagagaactt tcgccgtgaa cgctggatcg aagtcgatat
      721 ggcctggcat gagcatatct atgaaatgag cgccaatccg tttttgacct cattcgcctc
      781 gctattccat tcggtttatc acacttactt cacgtcaatt accagcgaca cagtgataaa
      841 gctggatctg caccaggcga ttgtcgatgc gattatccaa agcgatggcg acgcggcatt
      901 taaagcttgt caggcgctgc tacgctcacc agataagtga taacccgata acaggacatt
      961 gaatgagcga taaaaagaag cgcagtatgg cgggtttgcc gtggatcgcg gcgatggcct
     1021 tcttcatgca ggcacttgat gccactattc tgaataccgc cttacccgca atcgctcata
     1081 gccttaatcg ttctcctctc gcgatgcaat cagccatcat cagttatacg ctgacggtgg
     1141 cgatgcttat tccggtaagc ggatggctag ccgatcgctt cggtacacgt cgcattttta
     1201 cccttgccgt gagtctgttc acgttgggtt ctctggcctg cgcactttcc aactcgctac
     1261 cacagctggt tgtcttccgg gttattcagg ggataggcgg cgcaatgatg atgcctgttg
     1321 ctcggctggc cttactgcgc gcttatcctc gtaatgaact tcttcctgta ttgaattttg
     1381 tcgccatgcc gggtctggtg gggccaattt taggccccgt tcttggcggc gtgctggtca
     1441 cctgggcaac ctggcactgg atatttttaa tcaatatccc cataggtatt gcgggccttc
     1501 tttacgcgca caaacatatg cccaatttca ccaccgcacg acgcagattc gataccactg
     1561 gctttttgct gtttggcctc agccttgttc tcttctcaag cggaatagag ctattcgggg
     1621 aaaagattgt cgccagctgg attgccttga cggtaattgt caccagcatc gggttactgc
     1681 ttctctatat tctccatgcg cgacgcacgc caaacccatt aatttcatta gatttattta
     1741 aaacccgcac tttctcgatc ggtatcgtag gcaatattgc accccgtctg gggaccggct
     1801 gtgtaccgtt ccttatgcca ttgatgttac aggtaggatt tgggtatcag gcgtttattg
     1861 ctggctgtat gatggcaccg acagcgttag gttccattat tgcaaaatcg atggttaccc
     1921 aagtcttacg tcgtctgggc tatcgccata cgttagtggg gatcacggtg attatagggc
     1981 taatgatcgc tcagttctct ttgcaatcac cagcaatggc gatatggatg ctgatcttgc
     2041 cgttgtttat attagggatg gctatgtcga cacaatttac cgcgatgaat accatcacac
     2101 ttgccgatct gaccgatgac aacgccagca gcggtaacag tgttctggcg gtcacgcagc
     2161 aactgtctat cagtttaggc gttgctgtaa gtgcggccgt ccttcgcgtt tacgaaggga
     2221 tggaaggcac aacgacagtc gaacaattcc actatacgtt tatcacgatg gggattatta
     2281 ctgttgcttc agcagcaatg ttcatgctac tgaaaacaac cgatggtaat aatttgatca
     2341 aaagacagcg taaatctaag ccgaaccacg ttccatcaga atcggagtaa gttgtaatcg
     2401 ttgttgctgt agggtcggct gggttatccg atggatgagt acatcaatcg ccagctcccc
     2461 cagttcatct ttcggttggt gtatagttgt taatggtggc gtcataaagc ttgccagttc
     2521 gatatcgtca tagccaatca ccgcgatatc ctgcggaacc tgtaactctg cctgatataa
     2581 cgcctggtaa acgccaacag ccatagcgtc atttccggta aagacagcct gaggacgcag
     2641 cggatgtgat aacagttggc gcatggcgtc aaacccgccg ttaaattcaa aatcaccagt
     2701 gacttcatag ccatcaggaa tgttgagacc cgcacgtttc atcgccgccc gataaccttc
     2761 caaccgcagg cgcgccggag ttttatccag cggaccggta atacaggcaa tacgggtatg
     2821 acctttatcg atcagatatt gcgttgctaa gtctccgccc agcaacgagt tatcctgaat
     2881 aagatcgctg tcgccatcga acggagccca gtccatcatc acggtaggca ctgtcggata
     2941 acgttgcatg atttcacgcg aaggctgatg cgtttcggtg cacagtaaca gcaagccatc
     3001 aacgcgtttt tgcatcagag tttccagatt gcgattcatc cgctgttcat cgccttcggt
     3061 attgcaaagg acaagactat aaccgcgttc gaagcagctg cgttcaacgc cacgcaccag
     3121 ttctgaatag aaaggattgg tactggcagt gatcaacatg ccaatggtat gtgtttgatt
     3181 gagtttgagg ctacgcgcca gagctgatgg cgcgtaattg agttctttaa tcgccgcttc
     3241 aactttggct gtaatcgctt cactgacgaa gcgatcttta ttgataacgt gagaaactgt
     3301 tgaggtagaa acgcccgcca ggcgggcaac atctttcatt gtagccaagc gtcacctctg
     3361 cctgtctaaa aatgcgtcga tctcttcacg ccacggtacg gaaggttgtg cgcctttacg
     3421 tgttacggca atcgcagcgg ctgcatgggc aaaacgaatc gcctctggca atgttttttc
     3481 ttccagcaac gccgtgatta acgcgccgtt aaaggtatct ccggcagcaa tggtatcgac
     3541 agcctgcacc cggaatccag gaacgcgctg accttcacca ttcacgctag cccatacacc
     3601 acgacttcct aaagtaatca gtacagtacg gatacctttt tcatgcagtg cctgcgccgc
     3661 cttcgctgca tcttcatcat tttcaacacg aataccggtg agcttttctg cttccgtttc
     3721 gtttggcgta ataatgtcca ccagcgccag aagttcgtca ggaagttcgc gagccggagc
     3781 cgggttaagc gcaacgatag tcttattttg atgggcgatt ttcgccgctg ccataacact
     3841 ttcgagtggt gattccagct gcattaataa tgctgacgcg ttggcaatac gctcacgttg
     3901 cgcttccacc agcgccgggg aaagggcagc gttggccccg gcatgaatac cgatgacatt
     3961 ctcaccttcg ccattaacaa aaatcagcgc cacacctgtt gattcgcctt tgatcacgct
     4021 gagcggagaa atatcgatgt tatcagtggc taattgctga cgtacgctct cgccaatgct
     4081 gtcatcaccc gtacaggcaa taaacgcgat attcgcaccg ctacgcccag cagccaccgc
     4141 ctgattcgcg cctttgccgc caaatgcaac ctgatagtgg ttaccggtta cggtttcgcc
     4201 tggagtagga aaagattgaa gattaagaat gtggtcagca ttaatgctgc caagaacaac
     4261 gaggctgcct gcgttttgca tattcgggat gtccataaaa tgcgccaccg tgttagggtg
     4321 gcgcgtgccc tgcttttctt tatattacac gttcatctgt cgatgacgta tttatgtcac
     4381 catcaggtca tacaacctga ttaaaactac tgcttaacaa ccagtttcag atcaaccgga
     4441 tacttagcct gaactttctc gcctttcagc actttatctg cggtttcgac gcctttcgcg
     4501 ccaatctgat cgggtagctg agcgatagtc gctgctagtt tgccatcatt caccgctttt
     4561 tcgccatccg gtgtaccgtc aaatccgacg accatcacat ccgatttacc ggcagtttgc
     4621 agtgcgcgca gcgcgccaag cgccatttca tcattctgcg cgaatacagc ctgaacatcc
     4681 ggatgagcgg tcaacaggtt ctgcattacg ttcaaacctt tggtgcgatc aaaatctgca
     4741 ggctggctgg caagaacatt aaacttgtga gcagcaacgg cctgctggaa accttcgcca
     4801 cgttcacggg ctgcggatgt accggcaatg ccttgcagct cgataacttt ggcaccttca
     4861 cccactttct tcgcgatgta atcaccggcg attttgccgc ccagtacgtt atcagatgca
     4921 atgtggctca ccacttcacc tttcgttgcc tggcggtcaa gagtgataac cgggatgttc
     4981 gcctggttag ccatcttcac agcattacct actgcgtcgg agtcggtcgg gttaatcagc
     5041 agaattttgg tgccgcggac ggttaagtcc tgcacgttcg ccagctcttt cgccgggttg
     5101 ttctgggagt ccagcaccac caggttatag ccaagtttat ccgcctcttt ctgcgcgcca
     5161 tctttcagcg atacaaagaa cgggttgtta agcgtggaga ccaccagcgc gatggtgtct
     5221 tttgccatcg cattcgcact gacggtggcg cttagcgcaa cagcggaaac cagggtagcc
     5281 agttttttca tgttcatatt caagatgtcc tgtagtcgtt attactgctt tttgttgtct
     5341 accagcaccg ccagcaaaat caccaccgct ttgacgatca tctggtaata ggaggaaaca
     5401 cctaacaaat tcaatccatt attaaggaag ccaagaatta atgcgccgat caacgtccca
     5461 acaatgcgac cttttccgcc cgccagactc gtaccgccca gaaccaccgc agcaatagca
     5521 tccagctcat agccagtccc cgccgtgggt tgtgcggagg agagacgcgc cacttcaatg
     5581 atcccggcca gcgatgccag cagaccacaa agagaataga cgatgatttt gattttattg
     5641 acgttgatac cagaaagacg cgttgccgct tcattgccgc ccagcgcgta gatgtaacgc
     5701 cccagacgcg tgtgatgcag catgtaccag gccgcgagga agacaatccc catgatccag
     5761 actggcgtcg gtacgcccag cggacgacca ataccaaacc agccaaacag atcggcgttc
     5821 tcagtaaagc cggtattcac tgggctacca ttggtataaa ccatggtcac gccgcgcagt
     5881 aaaagcatca taaccagcgt agcgataaac gcctggacgc gacctttcgc tacaatcacc
     5941 ccagttaccg caccaattgc ggcacctaac gcgagagcgg cagcgacagc caccagcgca
     6001 ttgacttcaa tgccgacgat agatgcagca actgcgccgg tcagcgccaa cagagaacct
     6061 accgacaaat cgatacccga cgtcaggatc accagcgtca tcccgaccgc cataatggcg
     6121 ttcaccgagg tttgctgaag aatattgaat aagttattga tggtgaaaaa gttcgggctt
     6181 aacgtcgaga caatcgcgat cagcaccagc agagcgataa gcgatttctg ctccatcagc
     6241 cacgctttcg tgaaataacg gcgaccagag acagtctggg ttgtcatttt tttactcctg
     6301 attcacgcga ttaagcttgc ccacagcggc agccattaac acttcctggg tggcctgctc
     6361 acgagtaaat tccccgctga gatgcccttc atgcatgacg atgatgcgat cgctcatgcc
     6421 taatacttct ggcatctccg atgacaccag aatgatgctc aagccatcgg ctttgaactg
     6481 gttaatcagt tgataaatct cttttttcgc gccgacatct acgccacggg taggctcatc
     6541 gaggatcaac actttagggc gtgtcatcag accacgggca atcgctactt tttgctgatt
     6601 gccaccggaa agcagaccaa ttgcctgttc catcgatggc gttttcacat taaacagacg
     6661 aatgaaatca ctcacagcct gctgttcatc ggcatgcttc aaactgccgc cagcgcggct
     6721 gaagtagcgc agcgcggtca gcgacatgtt ctcttttact gacatgccca gcactaaacc
     6781 gtcacgttta cggtcttcgg agatataaac aatgccgttt gccaggccat cctgcggcga
     6841 acgggtaacg acttcatgcc catccagggt gacgtaaccg cttgtgcgcg gcagtgcacc
     6901 gtagagcact ttcatcagtt cggtacgccc agcgcccatc aaaccagaga cgccaagaat
     6961 ttcgcctttg cgtaaagtaa aagagacatc gttaacgcca ggtccgcaga gattatcgac
     7021 tttcaggcgg atatctcccg gcgctttgtc caggtgcgga tattgatctt ccagcttgcg
     7081 gcccaccatc atctcaatca gagaatcttc ggtcagtgat gccacttcgc gctcagcaat
     7141 aaattgtccg tcacgaaaaa cggtaacgtc atcgcaaatc tcgaagattt ctttcatgcg
     7201 gtgggagata tagacaatac cgcggccttg cgatttcagc tcgcggatga cgcggaacaa
     7261 ggattcggtt tcggtatcgg tcagcgcatc ggtcggttca tccataatga tgactttcga
     7321 ctcaaaactc agcactttgg cgatttcaac catttgctgg tcaccgatgg aaagatcgcc
     7381 caccagcttg tcgcttttaa agcgcaggtt aagtttagcc agcaatttat ccgcttcggc
     7441 atacatggtt ttccagtcaa ttttgccaaa gcgattaaca aactcacgac cgaggaaaat
     7501 gttttcggca atggttaact gcgggatcag gttcagttcc tggtggataa tcccaatccc
     7561 ggcttcctgg gaagatttcg gcccggtaaa tgtcgtttct ttccccaacc ataaaagcgt
     7621 accggcatcg cgagtataga taccagtaag cactttcatc atggtggatt tacccgcgcc
     7681 gttttcgccc accagcgcca tcacgcggcc cggatagaca tttaacgctg cgcccgagag
     7741 ggcttttacg cccgggaagg ctttatcgat gcctttaagc tgaagtaatg cttccatgac
     7801 ggcctcagaa cgtcacgcca gcacagagaa tgatattcgc atacggagaa cattctccgc
     7861 tgcgaattac cgcctgactt tctgcggttt gttgtttgaa ttgttcatgc gtggtgtaac
     7921 gaatttcaat ggtgtttccc tggtgttgtt gcagctgctc aaggtgagtg agcaacgttt
     7981 cgtggagttg cggattatgt tgtttgatct cttccgcgat aatgacagcc tcgacctgca
     8041 tttcatttgt gacgacgccc agcacctgca taaaagaagg tacaccctgg gttaatgcca
     8101 tatcgatacg cgttgtactt ttggggatgg gtaaaccagc atcacacacc accagcgtat
     8161 cggtatgtcc cagtcgggag atcaccgatg aaatatcaga attaagaacg gtgccttttt
     8221 tcattttttt ctccatcagc gaaacgtttc gctgacccgc agtttaatcc tgactgagtt
     8281 gggaaaacca tcgtgacgtt acggaaatgt gatcaacctc gaaacgtttt acatggtgat
     8341 taaccatgaa aacaaaaacg cccccttatg gagaaaggag gcgtctggcg ttagatttcg
     8401 acctgagtac ccagttcgat aactctgttt ggcgggattt caaattgatc cggtgcccgc
     8461 agcgcattac gttgcagcag caagtacagc ttgccgcgca gacgcaaata ccacgggcgt
     8521 ttgccgagga tcaacgactc atgcgacata aagaaggagg tttccatcat ccggcaactt
     8581 aatccttcca gaccgcagcg gtggaaaact tcttccacgt ttggcgtttc tcgccaacca
     8641 taacttgcca ccacgcgcca gaaagtgggc gacagttgtt caatctgtac ccgacggacg
     8701 ttatggacat atggcgcgtc ttcggtgcgc agagttaaca gaatcacccg ctcatgcaat
     8761 accttgttat gtttcaggtt atgcatcagc gcaaagggaa tgacgttgat tgcacgcgac
     8821 atatacaccg cggtcccagg cacgcgaacg ggcggtgatt tctccagcga agcaatcatc
     8881 gcttccagag agttaccatg ttcatgcatc cgccgcagca agcggaaacg ctcgctcttc
     8941 caggtggtca tcacgataaa catcaccgta ccgaggctca atggcaacca gccgccggag
     9001 agcagtttat cgaggtttgc ggtgaacaag ggaatatcga cacaaaggaa agcaatcagg
     9061 atcagcgcaa caaaatactt attccagtgc cagttctgac gtgccacggt agtcgagaga
     9121 atagacgtca gcaccatggt tccggtcacc gcaatcccgt acgccgccgc caggttgctg
     9181 gagtgctcaa agctgacaat cacaatcacg accgcgacat agagcatcca gttcacaaag
     9241 ggaatataga tttgccctga ctccatttcg gaagtgtgaa taatgcgcat cggcgacaaa
     9301 tatcccagac gtaccgcctg acgcgtcaat gagaagacgc cagagataac cgcctgcgag
     9361 gcaattaccg tcgccagtgc ggcgatgatc agcagcggga tcagcgccca gtccggtgcc
     9421 aacaggaaga acgggttctt aatcgcttcc ggattcttta acaacagcgc tccctggccg
     9481 aagtaattaa gggttaagga aggcaatacg acggtgaacc acgccagacg aatagggaac
     9541 ttaccaaagt gccccatatc agcatacagc gcctcgaccc ccgtaatcga cagcaccact
     9601 gcccctaatg caataaaaga aaccgttttg tattcaagga agaaatgcac cgcccacatc
     9661 ggattcagtg catgcagcac ttccgggtta gcaataatgc tacgtaaccc cagccctgcc
     9721 agaatcaaaa accaggtcag catgatcggc gcaaacagct taccgaccat agccgtgcca
     9781 tgtttttgaa tcatgaataa taatgtgaga acgataattg agaggggaac tatccaggta
     9841 tccagctgcg gggcgacgat ttccagacct tcaatggcgg acatcaccga aatagcggga
     9901 gtaatgacga cttcaccata gaaaaagctg ccgccgatta gccccataat caccagcatt
     9961 gatgtggttc gcgccgacgt attacgcccg gcaagcgaca tcaacgtcag gatcccccct
    10021 tcaccggcgt tatctgcccg catcacgaag gtgagatatt taatggaaac cacaaagatt
    10081 agcagccaga agatcagcga taaaaagcca aacacggcat cgcgttcaac gccaaaacca
    10141 aactggccgg acaaacattc acgaagtgta tataacgggc tggtaccaat atcgccgtag
    10201 acaactccaa tcgccgcgag ggtaatcgcg ggcaatgatt gcttattatc agtgctcata
    10261 gactagtctt tcgttgaaat atgaaatgtg tgcttagtcc cttggcccac aaaaaagcgc
    10321 acagtatgca cgattaacgg caaaatcgta ctcctaaatg cggccacatt aacctggctc
    10381 aaagaaaaat acccctcctt tgagactatt ttagccacca gcaagaaacg tctatactcg
    10441 caatttacgc agaacttttg acgaaaggac gccacttcat tatggctcac cctcatttat
    10501 tagcggaaag aatttcccgc ctgagcagtt cgttggaaaa ggggctttat gaacgtagcc
    10561 acgccatccg cttgtgttta ttagcggcat taagtggtga aagtgtgttc ctccttggcc
    10621 caccaggtat tgccaaaagt ttgatcgccc ggcgcttaaa attcgccttt cagaatgccc
    10681 gcgcgtttga atatctgatg acccgcttct ccacgccgga agaagttttt ggtccccttt
    10741 ctattcaggc gctaaaagat gaagggcgct atgaacgttt aaccagcggt tacctgccgg
    10801 aagctgaaat cgtctttctg gatgagatct ggaaagcggg tccggcaatt cttaataccc
    10861 tgctcaccgc cattaacgag cgccagttcc gcaacggcgc actcgtagaa aaaatcccga
    10921 tgcgtctgct ggtggcggcc tccaacgagc tgccggaagc agacagcagt ctggaagcgt
    10981 tatatgaccg catgctgatt cgtctgtggt tagataaagt gcaggataaa gcgaatttcc
    11041 gctccatgct gaccagtcaa caggatgaaa atgacaatcc ggttcctgcc tccttgcaga
    11101 tcacagatga agaatatgaa cgctggcaga aagagattgg tgaaattacg ctgcccgatc
    11161 atgtatttga gctgattttt atgctgcgcc agcaactgga taaattaccg gatgcgcctt
    11221 atgtctcgga tcgtcgctgg aaaaaagcga tccgcttatt gcaggccagc gcctttttta
    11281 gcggtcgcag tgctgtcgcc ccggttgatc tcattttgct gaaagattgc ctgtggtatg
    11341 acgcgcaaag cctgaatttg atacaacaac aaattgatgt attgatgacc ggtcacgcct
    11401 ggcaacagca agggatgttg acccgcctgg gcgcgattgt gcaacgtcac ctgcaacttc
    11461 agcagcaaca aagcgataaa acagccttaa cggtaattcg tctgggcggc atttttagcc
    11521 gtcgtcagca gtatcaactc cctgttaacg ttactgcttc cactctgact ctgctgctgc
    11581 aaaaaccgtt aaaactgcat gatatggaag tggttcatat ctcctttgag cgtagcgcgc
    11641 tggaacagtg gctgagtaaa ggtggtgaga ttcgcggcaa actgaacggt atcggctttg
    11701 cccagaaact gaatctggaa gtcgatagcg cccaacatct tgttgtacgc gatgtaagtt
    11761 tacaaggcag tacgctagca cttccaggtt catcggctga aggtctgcca ggtgaaataa
    11821 aacaacaact ggaagagctt gaaagcgact ggcgcaagca acacgcttta ttcagcgaac
    11881 agcaaaaatg tctgtttatc cctggcgact ggttaggtcg cattgaagcc agcctacagg
    11941 atgtcggcgc acagattcgc caggcacaac aatgctaacg ctggatacgc ttaatgtgat
    12001 gctggccgtc agcgaagagg gattgatcga agagatgatc atcgcgctgc tggcctcacc
    12061 gcagctggcg gtcttctttg aaaaattccc acgactgaag gcggcaatca ctgatgatgt
    12121 tccccgctgg cgtgaggcgc tgcgtaatcg gctgaaagat gcccgagtcc cacaggaact
    12181 caccgaagag gtgatgtgct atcagcaaag ccagctcctc tccacgccgc agtttattgt
    12241 gcagctacca cagatcctgg acttactgca tcgtctgaat tccccatggg cagaacaagc
    12301 ccgacagttg gttgatgcta acagcgcgat cacttcagcg ttacacacac tttttctcca
    12361 gcgttggcgt ttaagcctga tcgtgcaagc aacgacgtta aatcaacagc tattagaaga
    12421 agaacgcgaa caactgttaa gtgaagttca ggaacgcatg acgctgagcg gacaacttga
    12481 accgattctc gcagataaca ataccgcagc tggtcgtctg tgggatatga gcgccggtca
    12541 gcttaaacgt ggcgactatc agttgattgt gaaatacggt gaatttctta acgaacagcc
    12601 ggaactgaaa cgcctggcag aacaactggg gcgttcccgg gaagccaaat caataccgcg
    12661 taacgatgcg cagatggaaa ccttccgcac catggtgcgc gaaccggcga cggttcctga
    12721 gcaggttgat ggtctgcaac aaagcgatga tattttacgt ctcctgccgc cagaactggc
    12781 gacactgggg ataacggaac tggagtatga gttttaccgt cggctggtgg aaaaacagtt
    12841 gctcacctat cgcctgcacg gtgagtcgtg gcgtgaaaaa gtgatcgaac gcccggtagt
    12901 acataaagat tacgacgaac agccgcgcgg accgtttatt gtctgcgtgg atacttccgg
    12961 ctcaatgggc ggctttaatg aacagtgtgc gaaagcgttc tgcctggcct tgatgcgcat
    13021 tgctctcgct gaaaaccggc gctgctatat tatgctattt tccaccgaga tcgtccgtta
    13081 tgagctttca gggccacaag gtattgagca agcgatccgc tttttaagtc agcagtttcg
    13141 tggcggcacc gatcttgcca gttgttttcg cgccattatg gaacgcttgc aaagcaggga
    13201 atggtttgat gccgatgcgg tggtgatttc tgattttatc gcccagcggt tgcctgacga
    13261 cgtgacgagt aaagtgaaag agttgcagcg ggtacatcag catcgctttc atgccgtggc
    13321 gatgtcggca cacggcaaac ccggcatcat gcgcattttc gatcatatct ggcgctttga
    13381 taccgggatg cgaagccgcc tgctcagacg ctggcggcga taaattatta cagcagagaa
    13441 gggacgctct cgcgaacagc tgccggccat acaccacact gaacctggcc gatatgtggc
    13501 agttgcagca gcagcatggt caaacgagac tgcccgatac cgccgccgat ggtctgcggc
    13561 atttcaccgc gcagcagcgc ctgatgccac tccagctcca ggcgatcttc gtcaccggtc
    13621 agcgccagct gatgcttcag cgtgtcggca tccacgcgga tccccatgga agaaagctca
    13681 aacgcatctt ccagtaccgg gttccacacc agaatatcgc cgttcagacc cgcatggccc
    13741 agctctgacg gggtgctcca gtcatcataa tccggtgcgc gcacgtcgtg acgatgacca
    13801 tcactcaact tgccgccaat cccgacgagg aataccgcgc caagatcttt cgcaatcgcc
    13861 cgctcacgcc ctttggcatc gagatccgga taacgagaca gtaactcctg gctgtgtacg
    13921 aaatggatct gatccggcag gaacggtgcc aggccaaact cttcgctaac cgcagcttcg
    13981 gttgctttaa ttcccgccca gatcgcctct accgtgcttt taagagtcga gaattgacgc
    14041 tcgccgtcgc ccattacgcg ttcccagtcc cactggtcta catagaccga gtgcaacggg
    14101 gaaagacggt cttcatcggg gcgaagggct ttcatgtgcg tgtacagccc ttcgcccgcg
    14161 ctgaagtcgt gttgccctaa ggtctgacgt ttccacttcg ccagtgaatg aaccacttcg
    14221 aactgggcat caggcagagc tttcactttt acctgcaccg ctttttcaca gcccgacaag
    14281 ttatcctgcg tgccatctcc cacacggcta agaatcggcg cctggacttc gatcagcccc
    14341 agacgttctt ccagttgacg agaaaagtga gatttcacga agctaatttg acgttgtttg
    14401 gcaatgtaag cggttttcat tttttatact cctgcgtcct gttgcttatg attaagcaac
    14461 aaaaaccgac acacatgcaa taatcattca ataaaaagcc tcgcgtactt ttgattcatt
    14521 aaaaaagaag gctaaaatag aatgaatcat caatccgcat aagaaaatcc tatggaaaat
    14581 tatctaatcg acaatctgga ccgtggcatc ctggaagcat taatgggcaa tgcgcgcacc
    14641 gcttacgccg aactggcgaa acaatttggc gtcagtccgg ggacgattca cgttcgagta
    14701 gagaaaatga agcaggcggg gatcattacc ggggcgcgta ttgatgtcag cccgaagcag
    14761 cttggttatg acgtaggctg ctttatcggc attatattaa agagcgccaa agactatcct
    14821 tccgcgctgg caaagctgga aagcctcgat gaagtcactg aagcctatta cacaaccggc
    14881 cactacagca tctttataaa agtgatgtgc cgttcgatcg atgctctcca gcatgtactt
    14941 atcaacaaga tccaaacaat tgatgaaatt cagtccaccg agacactgat cgtcctgcag
    15001 aacccgatca tgcgtaccat taagccctga tcggcttttt taatcccata cttttccaca
    15061 ggtagatccc aacgcgttca cagcgtacaa tacgctactc ttaataaagg tggcggttta
    15121 tggcagatat cactcttatc agcggcagca ccctcggcgg tgccgaatat gtagcagaac
    15181 acctggctga aaagctggaa gagacgggtt ttaccaccga aacgctgcac ggtccgctgt
    15241 tggaagattt atctgcctcg gggatctggc tggttatcag ctcaacccac ggtgccggag
    15301 atattccgga caacctttct cctttctatg aagcattgca ggaacagaag cccgatcttt
    15361 ctgcggtccg ctttggcgca atcggtattg gtagtcgtga atatgacacc ttttgtgggg
    15421 ctatcgataa actcgaggca gaactcaaaa attccggtgc aaaacagaca ggcgaaacac
    15481 tgaagatcaa cattcttgat cacgacattc cggaagatcc ggcagaagaa tggctgggat
    15541 cgtggattaa tttactcaaa taagtataca gatcgtgcga tctactgtgg ataactctgt
    15601 caggaagctt ggatcaaccg gtagttatcc aaagaacaac ggttgttcag tttttgagtt
    15661 gtgtataacc cctcattctg atcccagctt atactgtcca ggatcaccga tcattcacag
    15721 ttaatgatcc tttccaggtt gttgatctta aaagccggat ccttgttatc cacagggcag
    15781 tgcgatccta ataagagatc acaatagaac agatctctaa ataaatagat cttcttttta
    15841 atacccagga tcccaggtct ttctcaagcc gacaaagttg agtagaatcc acggcccggg
    15901 cttcaatcca ttttcatacc gcgttatgcg aggcaatcac catgttttat ccggatcctt
    15961 ttgacgtcat catcattggc gggggtcatg caggcaccga ggccgcgatg gccgcggcgc
    16021 gtataggtca acagactctg cttttgacac acaatatcga cactctgggg cagatgagct
    16081 gcaacccggc gatcggcggt attgggaagg gacatctggt aaaagaagtg gatgcactcg
    16141 gcggtctgat ggcgaaagcg atcgatcagg cgggtatcca gtttaggata ctaaacgcaa
    16201 gcaagggacc ggctgttcgc gctacccgag ctcaggcaga tcgtgtgctc taccgtcagg
    16261 cggtacgtac ggcgctggag aaccaaccga acctgatgat cttccagcag gcagttgaag
    16321 atcttattgt cgaaaaagat cgcgtggtcg gtgccgttac ccaaatggga ctgaagttcc
    16381 gtgccaaagc tgtcgtgctc accgttggga cgttcctcga cggtaaaatt catatcggtc
    16441 tggataacta cagcggtggc cgtgctggtg atccgccgtc cattccgctt tctcgccgtt
    16501 tgcgtgaact gccgctgcga gttggtcgtc tgaaaaccgg gacaccaccg cgtattgatg
    16561 ctcgaaccat cgactttagc gtactggcgc aacagcatgg cgataaccca atgccggtat
    16621 tctcgtttat gggcaatgcg tcccagcatc cccagcaggt gccgtgttat atcactcata
    16681 ccaacgagaa aacccatgat gtgatccgca gtaacctcga tcgtagccca atgtacgcag
    16741 gggtgatcga aggtgtcggc ccacgctact gcccgtcgat cgaagacaaa gtcatgcgct
    16801 ttgccgacag aaatcagcat cagatcttcc ttgaaccgga agggctgacc tctaacgaaa
    16861 tttatccgaa cggtatctcc accagcctgc cgttcgatgt gcagatgcaa atcgtccgct
    16921 ccatgcaggg gatggaaaac gcgaagatcg tgcgtccggg ttatgccatt gagtatgact
    16981 tcttcgatcc acgcgacctg aaaccgacgc tggagagcaa gtttatccag gggctgttct
    17041 ttgctggtca gattaacggc actaccggtt acgaagaagc cgctgcgcaa ggtttgctgg
    17101 ctggtcttaa cgctgctcgt ctgtctgctg acaaagaagg ttgggctccg gcgcgttctc
    17161 aggcatatct cggcgtactg gttgatgacc tgtgcacttt aggaaccaaa gaaccgtatc
    17221 gtatgtttac ctcgcgcgca gaatatcgtc tgatgctgcg cgaagataat gcggatctgc
    17281 gtttgactga aatcggtcgt gaactgggcc tggtggatga cgaacgttgg gcgcgcttta
    17341 acgagaaact tgagaatatc gagcgtgagc gtcagcgtct gaaatcaacc tgggtaaccc
    17401 cgtcggcgga agctgcagcc gaagtgaatg ctcacctgac tgcgccgctt tcccgtgaag
    17461 caagtggtga agatctgctg cgtcgtcctg aaatgactta tgaaaaatta acctcgctga
    17521 caccgtttgc ccctgcgttg acagacgaac aggcggcgga acaggttgag attcaggtta
    17581 aatacgaagg ttatatcgcg cgccagcaag atgagatcga aaagcagctg cgtaacgaga
    17641 ataccctgct accagcgacg ctggattacc gccaggtatc cggtctttct aacgaagtga
    17701 tcgccaaact taacgatcac aaaccggcct ctattggcca ggcttcgcgt atttctggcg
    17761 tcacgcctgc ggctatctcc attctgctgg tgtggctgaa aaaacagggt atgctgcgtc
    17821 gtagcgcata acgcattaaa aatgcctggt aagcacccgc ttaccaggca acgcatcaag
    17881 aacaggtaat caccgtgctc aacaaactct ccttactgct gaaagacgca ggtatttcgc
    17941 ttaccgatca ccagaaaaac cagcttattg cctacgtgaa tatgctgcat aaatggaata
    18001 aagcgtacaa cctgacttcg gtccgcgatc ctaatgagat gctggtacgc catattctcg
    18061 atagcattgt ggtggcaccg tatctgcaag gtgaacggtt tatcgatgtc ggcaccggac
    18121 cgggactgcc tggcattcca ctctctatcg tgcgtcctga agctcatttc acgttgttgg
    18181 atagccttgg taaacgcgtg cgtttccttc gtcaggtgca acatgagctt aaactggaga
    18241 atattgagcc agtacagagc agggtagaag agtttccgtc agagccgcca tttgatggcg
    18301 taattagccg cgcttttgcc tctctgaacg atatggtgag ctggtgccac catcttcctg
    18361 gtgagcaagg ccgtttctac gcgctgaaag ggcaaatgcc ggaagatgaa atcgctttgt
    18421 tgcccgaaga atatcaggtc gaatcagtgg ttaaacttca ggttccagcc ctggatggcg
    18481 aacgtcatct ggtggtgatt aaagcaaata aaatttaatt tttatcaaaa aaatcataaa
    18541 aaattgaccg gttagactgt taacaacaac caggttttct actgatataa ctggttacat
    18601 ttaacgccac gttcactctt ttgtatcaac aagataacgt ggcttttttt ggtaagcaga
    18661 aaataagtca taagtgaaaa tatcagtctg ctaaaaatcg
//
LOCUS       DAZFJL010000093        18621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFJL010000093 DAZFJL010000000
VERSION     DAZFJL010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.093"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(50..331)
                     /locus_tag="ACHJ8C_003163"
     CDS             complement(50..331)
                     /locus_tag="ACHJ8C_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO5552973.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVIAADSDSKRRIVLNRYD"
     gene            complement(594..1703)
                     /gene="apbC"
                     /locus_tag="ACHJ8C_003164"
     CDS             complement(594..1703)
                     /gene="apbC"
                     /locus_tag="ACHJ8C_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO5552974.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1835..3868
                     /gene="metG"
                     /locus_tag="ACHJ8C_003165"
     CDS             1835..3868
                     /gene="metG"
                     /locus_tag="ACHJ8C_003165"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO5552975.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4009..7805
                     /locus_tag="ACHJ8C_003166"
                     /pseudo
     CDS             4009..7805
                     /locus_tag="ACHJ8C_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615297.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7818..11576
                     /locus_tag="ACHJ8C_003167"
     CDS             7818..11576
                     /locus_tag="ACHJ8C_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFO5552976.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQGNELHISWGKVGTQGQSQVKNFAD
                     AVAAEKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAET
                     RAADKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKER
                     EDVMDQIIQEGGLEYATDVIIHLQQISIEWDYVNNNIVFLSSGISPDYLQQYSSFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     SPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     VASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAALAALAELLVQK
                     EENSWRIMLMTMLISQPTLAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAIV
                     VSPPWLSKKKKSPIPVLDLAPLNLESICTITDTEAKEFQTHWDWEPHKPGEGAKDFLY
                     SLGYRRWDFDTYKYIGASDSAIDAWEREDFATLIQMFKAHHAPYQGEWHLNSLPFLPM
                     QKAIKLWEFLSKEPHTAIKPVMLYLRLAGMSGFLHSFSRYPQEGFAVANYFAATELAP
                     AVARAFNKLKTLRQDASSWLLKYPQHAITGLLPAALGKAGEAQDNARAALRMLTENGH
                     QPLLQEIARRYNQPEVTDAMNALLALDPLDNHPTKIPTLPTFYQPSLWTRPLSKANAQ
                     SLPDSALLHLGEMLRFPQEEALYPGLLQVKDACTTDSLAEFAWDLFTAWQTAGAPSKE
                     SWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIA
                     QKLKFKALQERAKEKIANIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVS
                     FDESVKPFVRDASGSRLKDLPKPNKTDDETLAEEAVNRYKLLKKDVRTVAAQQISRLE
                     AAMCQRRRWTAEQFSLFLVEHPLVRHITQRLMWGIYDADNQLTSCFRVAEDGSFSDGQ
                     DTPFTLEQGNIGIPHVLEIPTMQAAEFAQLFSDYELLPPFRQLDRPWSELSDSEKSSG
                     DLQRWAGRQAASGRVAGLMNKGWQRGDVLDGGGYYSFYKAVDDGYVELSVTPGFCVGL
                     PVTEISDSQTIDHIHLYKQTSRKSVYPFSVLDDITASELINDIESLFD"
     gene            11589..14621
                     /locus_tag="ACHJ8C_003168"
     CDS             11589..14621
                     /locus_tag="ACHJ8C_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFO5552977.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQGNELHISWGKVSTNGQSQVKSFAD
                     TAAAEKAELKLIAEKVKKGYVGQVDSNANSHDASAKKVIVIAEKASINHQASTNGSPW
                     LADNDPIILPPNIARYALSHRLWPGETVKKPTKPEKQNLISRLANRTYHYYKNLTFDY
                     SSCSDEWADAISQAISLIKAQKTEIPARTMAVLTLLEICFCQDWNGETSSELLDEIVS
                     TFGIEYATETVIFWWQIDFDFDYKNEHLTLFINHADYSRDSYRRNRDKFSFRLRKHLS
                     LAEKEVWNNCIAKLLVALPDISVYRQPLIAILMPEIPEVAHEIVQRLHQLADIPQLEM
                     LKLVATDPITLEILENYQHIDVFNTYGTSWSATVLQEQGITALARLVPYAQHDSCGDV
                     LTWVNHPQAVSALIEATDNNKRCYERLIKSAENFPSATIAALAEALVKKDDKRKHTLL
                     VSLLNANPLLAEKLVMRLSPHAADLVQQCQQLLNSRNEDFANSEMLPVILVSPPWATT
                     NKNNDNSPVGKYLMKIPALPLFYQPSLWTRPKLKNSSKVLPDNALLHLGEMLRFPLEE
                     ELYPGLLQVKDACTTDSLAEFAWDLFTAWQTAGTPSKESWAFTALGVLGNDDTARKLT
                     PLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIAQKLKFKALQERAKEKIADIA
                     ESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVSFDETLKPFVRDVSGSRLKDL
                     PKPNKSDDESQANDAVNRYKLLKKDARTVAAQQVARLESAMCLRRHWSPENFQLFLVE
                     HPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYSTADDDLFTLPEGDISIGIPHVLE
                     ISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAERNASELTRWTGRKCPSGRVMGL
                     ANKGWMRGEPQDGGWIGWMIKPLGRWSLIMEIDEGFAVGMSPAELSAEQLLSKLWLWE
                     GRAESYGWGSNSTQEAQFSVLDTITASELINDIEALFE"
     gene            14631..18263
                     /gene="yehI"
                     /locus_tag="ACHJ8C_003169"
     CDS             14631..18263
                     /gene="yehI"
                     /locus_tag="ACHJ8C_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO5552978.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSITARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIVRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NKFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALNLPEKPEIANELVSL
                     ECSQTHFRSKEWLKVVADDPKAVKELARYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKAPPARPGYPIIEDQKLPAQQKARDEYWHTLLQTLMASQPQLAEE
                     VMPCLSTQAQAVVNSYLSAPLKPVIDSTDNSCLPEILVSPPWRSKKKMTAPRLDLASL
                     ELTPQVYWQPGERERLAATESARYFSTGSFTERMERKSGRVVLQELGLGDDVWLFRNY
                     ILPGKLDAARKSLVVQWHYSPRRVEEINNGWHSTEAKSAEQALRSGDVEALINIWEND
                     SYSHYRQEKSVWNLYLLAQLPREMALTCWLRINEKKHLFAGEDYFLSILGLDTLPGLL
                     LAFSHHPKQTFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWPALEQLLKLGPIEIYPA
                     RIPKAPDFWHPVMWSRPRLIKNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRSLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALIFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLETALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMAAEMRSEFAQLF
                     ADYEIMPPFRQLARRTVLLTPDESASNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGAYRLVLKFSPGFNHYSVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            18324..18621
                     /gene="yehK"
                     /locus_tag="ACHJ8C_003170"
                     /pseudo
     CDS             18324..18621
                     /gene="yehK"
                     /locus_tag="ACHJ8C_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
BASE COUNT         4986 a         4693 c         4500 g         4442 t
ORIGIN      
        1 atataaatga tttaagcttt ctaaatccga ttaaaaccag ggtggcaaat taatcgtagc
       61 gatttaacac gattcgacgt ttgctatcgc tatctgcagc aatcacttcc gtcgttacac
      121 ctttaactgg cgaagaaacc tgatgacggg tatcaacatt atacgttaac tgaccacaat
      181 gctgttgagg ttgaccatta accatagcca tactgcatgt tgggggactg atcctaccat
      241 taatcgttaa tgtagcagtt tctgaagaga acgcgggaga agccaatcca taggcaagaa
      301 aaataattcc tgacagccag tacttattca ttgaacgtta tccctgtagt aaaggttatg
      361 cctggaaaga atgagtgcat aacaaactat agctgtacat ccactacaca gccacgaagg
      421 atgataatga agcatcgccg gtacgatcaa tcgactttgt agagtttcgg acgaaggtcc
      481 gcagaatatt cgcagcatta aataagtgtt cattaatgtt gcaaaaacaa atacaccgca
      541 tcaatgtaac atctctgcgg tgtatgaaga ataaccagaa tggttaatac gcgttagacc
      601 gcgcggaagg aaatctcgcc tggaatgact tcaccctgcc agtagagctg agctgcaacg
      661 cggtcagcca gctggcggta gatagcggta aattcgctct caggacggct gataaccgtt
      721 ggcgtgcctt tatcgagatc ctcacgtaag gagatgtgta acggcatctg ccccagcagc
      781 tgcgtgtggt atttctcggc cagtttctct gcaccaccgg taccgaaaat cggttcatgg
      841 tgaccacagt tactgcaaat atgcacactc atgttctcga caataccgag caccggaact
      901 tcgactttct cgaacatcac aatgcctttt ttcgcatcga tcaacgcgat gtcctgcggg
      961 gtggtgacca cgactgcacc ggttactgga atgttttgtg ccagtgtcaa ttgaatgtca
     1021 ccggtacctg gcggcatatc aagcaccaga tagtcgagat ccggccacaa ggtttcctgc
     1081 aacatttgca ttaacgcctt gctcgccatt gggccacgcc acaccatcgc attgtcgtct
     1141 gtcaccagat aaccaattga attagttgcc aggccatgag acataatcgg tgccatatgg
     1201 gtaccgtccg gcgaagttgg acgctgattt tctgcgccca gcatggtagg gattgatggg
     1261 ccgtagatat cggcgtccag aatgccgact ttcgcccctt ccgccgccag tgccaacgcc
     1321 aggtttaccg ccgtggagga tttacccacg ccacctttac cggaactgac cgcgataatg
     1381 ttcttcacgc cgttaattcc cggctgattc ttcacgcgtt tcagagtggc tatgttatgc
     1441 gacagtttcc agtcgatagc tttcgctccg gtgatgcgca gcagttcggc gctacattgc
     1501 tcttttaact cttcgaacgc gctatgccag acgaatggca taaccagttc cacatgcagc
     1561 gtgtcgtcca tccaggcgac atggtgcaac gctttcagcg ttgtgaggtt atgcttcagg
     1621 gttgggtgct gaaaattggc cagtgtcccg gcgaccattg cgcgcaaggc ttctggtgat
     1681 ttggcctggg attgttcgtt catcccgact ccttttttgt atagataaac catcagttga
     1741 tagtttacct gaagaatata gagaagtact tacttaacat tttcccattt ggtactatct
     1801 aagccctttt cactattaag aagtaatgcc tactatgact caagtcgcga agaaaattct
     1861 ggtgacgtgc gcactgccgt acgctaacgg ctcaatccac ctcggccata tgctggagca
     1921 catccaggct gatgtctggg tccgttacca gcgaatgcgc ggccacgagg tcaacttcat
     1981 ctgcgccgac gatgcccacg gcacgccaat catgctgaaa gctcagcagc ttggtatcac
     2041 cccggagcag atgattggcg aaatgagtca ggaacatcag actgatttcg caggctttaa
     2101 catcagctat gacaactatc actcgacgca cagcgaagag aatcgccagt tgtcagaact
     2161 tatctactct cgtctgaaag aaaacggttt tattaaaaac cgcaccatct ctcagctgta
     2221 cgatccggaa aaaggcatgt tcctgccgga ccgttttgta aaaggcacct gcccgaaatg
     2281 taaatccccg gatcaatacg gcgataactg cgaagtctgc ggcgcgacct acagcccgac
     2341 tgaactgatc gagccgaaat cggtggtttc tggtgctacc ccggtaatgc gtgattctga
     2401 acacttcttc tttgatctgc cctctttcag cgaaatgctg caggcatgga cccgcagcgg
     2461 tgcgttgcag gagcaggtgg caaataaaat gcaggagtgg tttgaatctg gcctgcaaca
     2521 gtgggatatc tcccgcgacg cgccttattt cggttttgaa attccgaacg cgccgggcaa
     2581 atatttctat gtctggctgg acgcaccgat tggctatatg ggctctttca agaatctgtg
     2641 tgacaagcgc ggcgacagcg taagcttcga tgaatactgg aagaaagatt ccaccgccga
     2701 gctgtatcac ttcatcggta aagatatcgt ttacttccac agcctgttct ggccagcaat
     2761 gctggaaggc agcaacttcc gcaagccgac caacctgttt gttcacggct atgtgacagt
     2821 gaacggcgcg aaaatgtcca agtctcgcgg cacctttatc aaagccagca cctggttgaa
     2881 tcattttgac gctgacagcc tgcgttacta ctacactgcg aaactctctt cgcgcattga
     2941 tgatatcgat ctcaacctgg aggatttcgt tcagcgtgtg aatgccgata tcgtgaacaa
     3001 agttgttaac ctggcgtccc gtaacgctgg ctttatcaat aagcgttttg acggtgtgct
     3061 ggcaagcgag ctggctgacc cgcagttgta caaaaccttc actgatgccg ctgaagtgat
     3121 tggtgaagcg tgggaaagcc gcgaatttgg taaagctatc cgtgaaatca tggcactggc
     3181 tgacctggct aaccgctatg tcgacgagca ggctccctgg gtagtcgcaa aacaggaagg
     3241 tcgcgatgct gacctgcagg cgatttgctc aatgggcatc aacctgttcc gcgtgctgat
     3301 gacttacctg aaaccggtac tgccgaaact gactgaacgt gcagaagcat tcctgaatac
     3361 agaactgacc tgggatggta tccagcaacc gctgctgggc cataaagtga atccgttcaa
     3421 ggcactgtat aaccgtatcg atatgaaaca ggtggaagca ctggtggaag cctctaaaga
     3481 agaagtgaaa gccacagccg cgccagtaac tggcccactg gcagatgatc cgattcagga
     3541 aaccatcacc tttgacgact tcgccaaagt tgacctgcgc gtggcgctga ttgaaaacgc
     3601 agagtttgta gaaggttctg acaaactgct gcgcctgacg ctggatctcg gcggtgaaaa
     3661 acgcaatgtc ttctccggca ttcgttctgc ttacccggat ccgcaggcac tgattggtcg
     3721 tcataccatt atggtggcta acctggctcc gcgtaagatg cgcttcggta tctccgaagg
     3781 catggtaatg gctgctggtc ctggcgggaa agatatcttc ctgctaagcc cggatgccgg
     3841 tgctaaaccg ggtcatcagg tgaaataatc ccccttcaag gcgctgcatc gacagcgcct
     3901 tttctttata aattcctaaa gttgttctct tgcgattttg tctctctcta acccgcataa
     3961 atactggtag catctgcatt taactggata aaatcacagg gatgcagaat gagacacttt
     4021 atctatcagg acgaaaaatc acataaattc tgggcggtgg agcaacaggg aaataaactg
     4081 catatcagtt ggggaaagat cggtaccaac gggcaaaacc aggtaaaaag ttttgcagat
     4141 gctgcggcga cggaaaaagc ggaactgaag ctgattgccg agaaaacaaa aaaaggctat
     4201 gtggaaggcg attcagcaaa tgtgaatatc ccccccatca ccaaagccac tcctgaagtt
     4261 gaaacttctc ctgagagtaa aaaccaacgc ccctggctgg ctgatgatgc ggtcattcgg
     4321 ctaactgacg atattaatcg atttgctttt ccacatcgct ctcgccccag agagatcaat
     4381 tatctgcaca aagatggtca aatatggaag cgcatcgcga ataacaccag ggcatatgac
     4441 cccgacaata attaccgctc ttatcctgaa aattggcagc aagcgtttgc cgagttacaa
     4501 agtcgaattc tggacaatca acaaacaggg agtgcgcaat ctgatgcggc attgctctgg
     4561 agtttttgga actcttactc tgcagatgaa ctggtcgatg acttagtcat tcgctgtggt
     4621 ctggaaagtg ccgttgaaat cgcccttttt gctcttcaac tcaaatataa accagtaaaa
     4681 ggcgcagtca ccaccatcat tccacctggt cacaaagcag aatcgctacc gaattggcat
     4741 caacgtctat gtgatcatct ttcactcgct tcagaagatg agtggcaacg ctgtatagat
     4801 aaagtactcg ccgccattcc gacgctatcc cccgcacggc aaccttttgc ggcgttactg
     4861 ctccctgaac gcccggatat tgccaatgca atggctctac gctatgcaga ccaaaacgtt
     4921 ccagcgatga cctggttaag actggtggta accgatgacg ccgctctgac tacagcagaa
     4981 aaatacgact tcccaccgtt gtatcatgat tttcgcgctt atctggcaat gttgctggca
     5041 aataatgggg tgcgcggtgt aagccggata cttcttgagt ttacggaaga gcattctgac
     5101 aatcccactt atctcttcga gcgcatcagc gagactgaag atcttgtcaa atggctatgg
     5161 aaaacgaatc acccggatgc gattcaaatt ctgatcctcg gtgtgattgg caagaaaaag
     5221 cacctggaat acttaagcaa agcctgtcaa aaacatcccg ctgcggctct tgccgcttat
     5281 gctactttgc tggcaataca tgaagacaaa gagtggcgta aagcgcttgt caaactgatt
     5341 accgtcacgc cagagttagt cactgacgtc attcccaggg ttaatgctaa agcggcaggc
     5401 atcttatctg aatgtcgccc gcagccagta actgaagaat gtgaatatgc cactgtggat
     5461 atgttgccgg agctgtttgt cgctccaccg tgggtaataa ataagaaaaa gaatgtaata
     5521 cccgtatttg atttgccggt acttcccatc cctgccgtca ctgatatcac accaggaatt
     5581 acagagttaa tcagtcatac tgacatatcc agattcagtg aaatcgcgca atatcaggca
     5641 tcacagcaaa cgttatttac cgacctgcca ccgataaaaa aagaacgctg ggaaacgact
     5701 ttcatccctt taacaccaga acagcaaatc ctttggcgac tggggtttaa ggaatggcgt
     5761 agaaccggag aagaacaata cgaaaaaaaa taatgccaca aagcgtagtt gatgccctgt
     5821 tacgtttcga tttccccgcg ctgaaagccg agtttgcgca ataccataac aaaggaagca
     5881 gacactggca gttgtacgcg ctctgcttcc tacccactca gcatgctatt tcttttcttg
     5941 atcaaataat taatgaagag caattctccg gtgagagaga aattctggct atttttggtg
     6001 atgcggccat tcctgcgttt atgaaatgtc tgcaacgtaa gccacaacag ttgtggatat
     6061 tcaccctctt cctgggcgtc agcgaattag cattaccgat ggcgcagcga ttacagaaaa
     6121 aaatgtctgc tgaagatgcc cgaaaatggc tggctaactt cccgcgccat gccgccgccg
     6181 ggctacttcc tgtggccctg ggcaaaaaag gaaaagatcg tgattgtgcc cgccaggcgc
     6241 tccgcctgct ggtgaattta aaccaacgag aaacgattga ggaaatcgcc cgggggtata
     6301 accaaccaga tgttttagca gcgctggcaa cattatttga tagcgatccg cttgaggagc
     6361 atcccgcaaa aatcgcccca ctgcccggct tttatcagtt caccttatgg cgcagaccac
     6421 ggcttaaaag taacaacctg cctctgccag atgacgctat gtgccacctc ggcactatgt
     6481 tgagctttcc acgcgacatt accgcctacg ctggtctgga gatcatcaaa gacaccttca
     6541 cccgcgaatc actggctgac tttggctggg atctgtatac cgcctggacg gaggctggcg
     6601 ctcccgcaaa agaaaactgg gcatttactt cgcttgggat tttaggcaat gacgacaccg
     6661 cgcgtaaatt aaccccgctt atccgtgcct ggcctgggga atcccagcat aaacgggcgg
     6721 tttacgggct ggatgtgtta gccagtatcg gtagcgacat agcgctaatg ttgcttaatg
     6781 gcattgcgca aaagattaaa ttcgttgcat tacaggaaca tgccagcgac agaatcaata
     6841 tggtcgccga aaatcgcgga ctgacaatgg ctgaactgga agaccgcctg gctccagatt
     6901 tagggcttga tatcaacggc tcgctgatac tggatttcgg cccccgcaaa ttcaccgttg
     6961 gttttgacga aaccttaaaa cctgtagtgc gcgatgcaaa cggcaaagtc ctgaaagatt
     7021 tacccaaacc aaatcagagc gatgataaaa cactggcaac tgacgccgtt aacctcttta
     7081 aacaattgaa gaaagatgtg cgcgccattg ccagccagca gattgatcgt ctggagcagg
     7141 ctatgtgcca gcgcagacgc tggacggcag agcagttccg cctgtttctg gtggagcatc
     7201 cgctggtacg tcacttaacc cgacggctgt tatggggggt atatactgag gaaaacacgc
     7261 tgatcgcctg cttccgggtc gcagaagaca gcacttacag cgatgcgcag gatgagctat
     7321 ttacgttgcc agcaggaaac atcggtattc cgcatgtgct ggaaatatct cctgaatccg
     7381 ccgctgcatt tgggcaaatt tatgctgatt acgaaaagct tcctcccttt cgccaactcg
     7441 atcgtggtta ttaccacctt actgataatg aaagtgatac ccatgaattg atacgctggc
     7501 aaggacgtct ttgccaggcc gggcgcattg tcgggctgga acgtcgaggc tggcaacgtc
     7561 tggaagaaag cggcagcgtc tatgcgatgc gcaaatctac cccttatggc gctctcgaac
     7621 ttgaaacaga acatttttca ttaatttatg gcgaaacggg gtatagcgac ctgctacctg
     7681 ttgaaagcgt aaaaatcact tcaccagggg aacgctacag tacacagccc tcgctcacgt
     7741 tctccgcgct tgatgccatc accgccagcg aactcattaa cgacatcgaa tcactttttg
     7801 attaaaagga ctttgcaatg aaaacctata tttatcagga cgaaaaatca cataaattct
     7861 gggcggtgga gcaacaggga aacgagttgc atatcagttg gggaaaagtt ggtacccaag
     7921 ggcaaagcca ggtaaaaaat tttgctgatg ctgtggcggc ggaaaaagcg gaactgaagc
     7981 tgattgcgga gaaggtgaag aagggatatg tggagcaagc gaaggataat tctttgcaac
     8041 cttcccaaac ggtaacggac tctctcaagg tagcggactt atccaccatt attcaggaac
     8101 aaccctcttt cgtagcagaa acccgtgcgg ctgacaaaaa tacagatgct gttttaccgt
     8161 ggctggcgaa agatattgct gtcgtttttc cgcccgaagt tgtacacacc acgttaagtc
     8221 atcgccgctt tcccggagtt cctgttcagc aagcagacaa attgacccaa ttacgtcgct
     8281 tagcctgtag tgtgtcgcaa cgggataata aaacagccac atttgacttc agcgcctgtt
     8341 ctttagaatg gcaaaacacc gtcgcccagg cgatcagtca gatcgacggc ctgaaaacaa
     8401 cacagctacc atcacccgta atggctgtac tcacggcact tgaaatgaaa tgcacaagat
     8461 ataaagagcg tgaggatgtt atggatcaga ttattcagga aggcggtctg gaatatgcta
     8521 ctgatgtaat aatacatctt cagcaaatat ctattgaatg ggattatgtc aacaataata
     8581 ttgttttcct gtcgtctggc atttcacctg attacttgca gcaatattcc agctttgaat
     8641 tacgactacg taaacattta tcactggcgg aagagtctct ctggcaaaaa tgtgcacaaa
     8701 aacttattgc cgcaattcca catattccag aatggcggca accattaatt gctttgttat
     8761 taccagaaaa accagaaatt gcacatgaaa ttgcccagcg tctactgggg caaaaaaaat
     8821 caccctcgct tgagtggcta aaaatagtgg cgactgatga gcacattctt gcctcattag
     8881 aaaaatatca cgagccatat gccatttttg atgattacta ttgtggtgcg atatggtcag
     8941 ccaccgtatt acaggagcaa ggtgttgcag ccctgccccg atttgctccc tatgtcgcaa
     9001 gtgactactg cgccgatgtg ttgcgtcata tcaatcatcc gttcgcatta acactgctta
     9061 tacgtgtagc cgggcatact aaacgctgtc acgatcgaat gacgaaagcc tgcgctgcgt
     9121 tcccacacgc agcactggca gcactggcgg aacttctagt gcaaaaagaa gagaatagtt
     9181 ggcgcattat gctaatgacg atgcttatct cgcaacctac actagcagaa caggtcattc
     9241 cctggctttc gacacccgca gttgcagtgc tgaaatcatg ccagcaacaa ctgacacagc
     9301 cctcaaacca tgccagcgcc gatctactgc cggccatagt agtctcccca ccatggcttt
     9361 cgaaaaagaa aaaatcgccg attccggtgc ttgatttagc accgctcaac ctcgaatcca
     9421 tatgcacaat aaccgacaca gaagctaaag agtttcaaac tcattgggac tgggaaccgc
     9481 ataagccagg tgaaggtgct aaagatttcc tttactcgct ggggtatcgc cgctgggatt
     9541 ttgacacata taaatatatc ggcgcttctg actcggcaat tgacgcatgg gaacgtgagg
     9601 acttcgctac actcatccaa atgtttaaag cccaccatgc accgtatcag ggggagtggc
     9661 atctcaactc acttcccttt ttacctatgc aaaaagcgat taagctatgg gaatttctca
     9721 gcaaagagcc gcataccgcg ataaaaccag tcatgcttta tttgcgactg gcaggaatga
     9781 gtggttttct acactcgttt tcacgctacc ctcaagaagg ttttgcggtt gctaattatt
     9841 ttgccgcaac ggaactggca cctgccgtcg cccgcgcctt caacaaactc aaaacattac
     9901 gacaagacgc cagtagctgg ctgctgaaat acccgcaaca cgccattacc ggcctgctgc
     9961 ctgcagcgct cggcaaagcc ggtgaagcac aggataacgc ccgcgctgcc ttgcgtatgc
    10021 tcaccgaaaa cggtcatcag ccattgctgc aagaaatcgc ccggcgttat aaccagccgg
    10081 aagtaacaga cgcgatgaac gctctgcttg cgctcgatcc cttagataat cacccgacaa
    10141 aaatccccac tcttccgacc ttttatcagc catcgctctg gacgcgcccg ttatcaaaag
    10201 caaacgccca atcactgcct gatagcgcac tcctccacct cggtgaaatg ctccgcttcc
    10261 ctcaggaaga ggcactgtat ccgggattat tgcaggtgaa agatgcctgt accaccgact
    10321 cactggctga atttgcctgg gatctgttta ccgcctggca gaccgctggc gcgccgtcga
    10381 aagagagttg ggcgttcact gcgttaggcg ttctcggtaa cgatgacacc gcccgcaaac
    10441 tgacgccatt aatccgcgcc tggcctggcg aatctcagca taaacgtgcc accgttgggt
    10501 tggatatcct cgccgctatc ggtagtgata tcgccctgat gcagcttaac ggcatcgccc
    10561 agaaactgaa attcaaagca ttacaggagc gggcaaaaga aaaaatagcc aacattgccg
    10621 agagccgcga actcacggtg gcggagcttg aagatcggtt agcaccggat ctcggtctgg
    10681 atgataacgg ttcgttgctg ctggatttcg gcccacgcca gttcaccgtc agctttgatg
    10741 aatccgtaaa accgtttgtg cgcgatgctt ccggcagccg tctgaaagac ctgcccaaac
    10801 cgaacaaaac cgatgatgaa actctggctg aggaagcggt aaatcgctac aagttattga
    10861 aaaaagatgt ccgcacggta gctgcacaac agatttcgcg tctggaagct gccatgtgtc
    10921 agcgtcgccg ctggactgca gaacagttca gcctgtttct ggtggagcat cccctggttc
    10981 gtcatatcac ccaacgtctg atgtggggca tttatgacgc tgataatcag cttacttcct
    11041 gttttcgcgt ggcggaggat ggcagtttta gcgacggtca ggataccccg tttacccttg
    11101 agcagggcaa tatcggtatc ccgcatgtgc tggaaatccc gaccatgcag gctgctgaat
    11161 ttgcccaact gttctctgac tacgaactcc tgccaccgtt ccgtcaactt gatcgcccct
    11221 ggtctgagct ctctgattca gaaaaaagca gcggcgattt gcagcgctgg gctggtcgcc
    11281 aggcagcgag tgggcgcgtc gccgggctga tgaataaagg ctggcaacgg ggcgatgttc
    11341 tggatggtgg cggctattac tcattttata aagctgtgga tgatggctac gtcgaactga
    11401 gtgtgacacc cggcttctgt gttggattgc ccgtcaccga aatcagcgat agccaaacaa
    11461 ttgaccacat tcatttgtac aaacagacgt cgcgcaaatc ggtttatccc ttctccgtgc
    11521 tcgacgacat caccgccagc gaactcatta acgatattga atcactgttt gattaaaagg
    11581 atttagcaat gaaaacctat atttatcagg acgaaaaatc acataaattc tgggcggtgg
    11641 agcagcaggg aaacgagctg catattagct ggggtaaagt cagcaccaat gggcaaagtc
    11701 aggtaaaaag ctttgctgat actgcggcgg cggaaaaagc ggaactgaag ctgattgcgg
    11761 agaaggtgaa gaagggatat gtggggcaag tagactctaa tgctaactct catgatgcct
    11821 cagcaaagaa agtcattgtc atagcggaaa aagcctcaat aaatcatcag gcatcaacta
    11881 atggttctcc ctggctggca gataacgatc ccattattct tccgccgaat attgcccgct
    11941 atgccctcag ccaccgcctt tggccgggag agacagtaaa aaaacccacc aaacccgaga
    12001 aacaaaattt aatttcccgg ctggcgaatc gtacttatca ttattataaa aatctgactt
    12061 tcgattacag ctcatgctct gatgaatggg ctgatgcgat tagccaggca atcagcttaa
    12121 ttaaggccca aaagactgag atacccgcgc gtactatggc ggtacttacc ttactggaaa
    12181 tttgtttttg tcaggactgg aatggtgaaa cctcatccga gttgctggat gaaattgtaa
    12241 gcaccttcgg tattgaatac gcaactgaaa ctgtcatctt ctggtggcaa attgatttcg
    12301 attttgacta taaaaatgaa catttgactc tctttattaa tcatgcagat tacagcaggg
    12361 acagttatag aaggaataga gacaaattta gtttccgtct acgcaaacat ttatcgctgg
    12421 ctgaaaagga agtctggaat aactgtattg caaaattact cgttgcgtta ccagatatat
    12481 cagtctatcg ccaaccgtta attgctattt taatgccaga aatacccgaa gtggcccatg
    12541 agatcgtaca acgattgcac cagctcgccg atattccaca actggaaatg ttaaaactgg
    12601 tagcgacaga tcctatcacg cttgaaatac ttgaaaacta tcaacacatt gatgtgttta
    12661 acacctatgg taccagttgg tcagctacag tattacagga acaaggcata acggcactag
    12721 ctcgcctggt tccctatgct caacatgaca gttgcggcga tgtacttaca tgggtcaacc
    12781 atccccaggc tgtttcagcc ctaatagaag ccaccgataa taataaacgc tgttatgaac
    12841 gactaataaa aagtgccgaa aattttcctt ctgccacaat agcggcatta gccgaagcat
    12901 tagtaaaaaa agatgataaa cgcaaacata cattgctggt ttcattactc aatgcaaatc
    12961 ctttattggc cgaaaaatta gtcatgaggt tatcgccaca cgccgcggac ttagtgcaac
    13021 aatgccagca attactgaat agccgcaatg aagattttgc aaactcagaa atgctaccag
    13081 tgatattagt ctcgccgccc tgggcaacga caaataaaaa taacgacaac agtcctgtgg
    13141 gtaaatacct gatgaaaata cccgctttac cgttgtttta tcaaccctca ttatggacac
    13201 gcccgaaact caaaaatagc agcaaggtac tgccagataa cgccctcctc cacctcggtg
    13261 aaatgctccg cttccccctg gaagaggaac tgtatccggg attattgcag gtgaaagatg
    13321 cctgtaccac cgactcactg gctgaatttg cctgggatct gtttaccgcc tggcagaccg
    13381 ctggcacgcc gtcgaaagag agttgggcgt tcactgcgtt aggcgttctc ggtaacgatg
    13441 acaccgcccg caaactgacg cctttgattc gcgcctggcc tggtgaatcc cagcataaac
    13501 gcgccaccgt cgggttggat attctcgctg ctatcggtag tgatatcgcc ctgatgcagc
    13561 ttaacggcat cgcccagaaa ctgaaattca aagcattaca ggagcgggca aaagaaaaaa
    13621 ttgccgacat tgccgagagc cgcgaactca cggtggcgga gcttgaagat cggttagcac
    13681 cggatctcgg tctggatgat aacggttcgc tgctgctgga ttttggccca cggcagttca
    13741 ccgtcagctt tgatgaaacc ttaaaaccgt ttgtgcgcga tgtttccggc agccgcctga
    13801 aagacttacc taaacccaat aaaagcgatg atgaatcaca ggcgaacgat gctgttaacc
    13861 gctacaaatt gctgaaaaaa gatgcgcgta ccgtcgccgc ccagcaggta gcaaggctgg
    13921 aatccgccat gtgcctgcgt cgccactggt caccggaaaa ctttcagctc ttcctggttg
    13981 agcacccgct ggtccgccac ttaacccgcc gtctgatttg gggtgtttat agcgccgaaa
    14041 accagctgct ggcttgcttt cgcgtagcgg aagataacag ctacagcacc gctgacgatg
    14101 atcttttcac cctgccggaa ggcgatatct ctatcggcat tcctcacgta ctggaaatat
    14161 caccgacgga tgctgccgcc ttcgggcagc tttttgccga ctacgaactg ttaccaccgt
    14221 tccgccagct tgatcgtaac agttacgcgc tgacagaagc cgagcgcaac gccagcgaac
    14281 tgacacgctg gacaggcaga aaatgcccga gtggtcgggt aatggggctg gcgaataaag
    14341 gctggatgcg tggtgaaccg caggacggtg gctggatcgg ctggatgatc aaacctctgg
    14401 gccgctggtc gttaatcatg gaaatcgatg aaggttttgc ggtcggcatg tcgccagccg
    14461 aactcagtgc cgagcaactc ttaagcaagc tgtggctatg ggaaggcaga gcagaaagct
    14521 atggctgggg gagtaattca acacaggaag cgcagttctc cgtactcgat accatcaccg
    14581 ccagcgagct aattaacgat attgaagccc tgtttgaata aggaaagagc atggacaagg
    14641 aattaccgtg gctggcggat aacgcccaac tggaactgaa atataaaaaa ggcaaaacac
    14701 cactcagcca tcgcaattgg ccaggcgaac cggtgcctgt tatcactgaa agcatcattc
    14761 agacactggg tgatgaattg ctacaaaaag cagagaagaa aaaaaacatt gtctggcgtt
    14821 atgaaaactt ttcactggag tggcagtccg ccatcacgca ggccatcaac ttgatcggcg
    14881 aacacaaacc ctcaatcacg gcccggacaa tggcggcgct agcctgtatc gcgcaaaatg
    14941 atagccaaca gttgctcgac gaaatcgtcc aacaagaggg gctggaatat gcaactgagg
    15001 tggtgattgc acgccagttt attgtacggt gttatgagag tgatcctctg gtagtaacat
    15061 tgcaatatca gaacgaggac tatggctatg gttatcgctc agaaacctat aacaaattcg
    15121 atctccgatt gcgtaagcat ctctctctgg cagaggaaag ctgctggcag cgttgcgccg
    15181 acaaactcat tgccgcactg ccaggaataa ccaaagttcg ccgccctttt attgcgctga
    15241 acctcccgga aaaacccgaa atcgccaacg agctggtaag ccttgaatgt tcgcaaactc
    15301 attttcgctc aaaagagtgg ttaaaagttg ttgctgatga ccctaaggcg gtgaaagaac
    15361 tcgcacgtta ctggagccag gatatattta gcgatcgaga agccagctac atgtcgcatg
    15421 aaaaccactt cggctacgcg gcctgcgctg cccttttgcg cgaacaagga ctggcagccg
    15481 ttccgcgcct cgcgatgtat gcacataaag aagattgcgg cagtctgctg gtacaaatta
    15541 accatccgca agtcatccgc accttgctac tggtggctga taaaaacaaa cccagcctgc
    15601 aacgtgtggc taaatacagt aaaaacttcc cccatgcgac gctcgccgca ctggcagaac
    15661 tgctggcgtt aaaagccccc cctgcgcgcc ctggttatcc catcatcgaa gaccaaaagc
    15721 tgcctgcaca gcaaaaagca cgtgatgaat actggcatac gctgttacaa acgctgatgg
    15781 catcgcagcc acaactggca gaagaggtga tgccgtgctt aagtacacaa gcccaggcag
    15841 tggtgaatag ttatttatcg gcaccgctaa aaccggttat tgatagcacc gataatagct
    15901 gtctgcctga aatcctcgtt tcaccaccgt ggcgtagtaa gaaaaaaatg acagctccac
    15961 gtcttgattt ggcatcgctc gaattaactc cgcaagttta ctggcaacca ggcgaacgag
    16021 agagacttgc cgccacagag tctgcccgtt atttcagcac gggatctttt acggaacgca
    16081 tggaacgaaa aagtggtcga gttgtactac aggagctagg tcttggggat gatgtatggc
    16141 tgttccggaa ttatatactg cccggcaaac tggatgcggc gcgcaaatca ttagtggttc
    16201 agtggcatta ctccccgcgc agagtagagg aaatcaacaa tggctggcac tccacggaag
    16261 caaaatcagc cgaacaggca cttcgttccg gcgacgttga agcgttaatt aacatctggg
    16321 aaaatgatag ctactcgcat tatcgtcagg aaaagagtgt ctggaacctg tatttattgg
    16381 cacagttgcc gcgtgagatg gctttgactt gctggctgcg tatcaatgag aaaaagcatc
    16441 tgttcgcggg tgaggactat tttctcagta tcctcgggtt ggatacatta cctggcctgt
    16501 tattggcttt ttcacatcat ccaaaacaaa catttccgtt aattttaaat ttcggcgcaa
    16561 cagaactggc cctgcccgtt gcccgcgtct ggcgacgttt cgcggcgcag cgtgatctgg
    16621 ctcgccagtg gattttacaa tggccggaac atacggctac tgcacttatc cctcttgtct
    16681 ttacaaaacc cagcgataat agcgaagctg cattacttgc cctgcgttta ctgtatgaac
    16741 aagggcatgg cgaattactg caaaccgtgg caaatcgctg gcagcgtaca gatgtatggc
    16801 ctgccctgga gcaattactt aaactgggtc caatagaaat ttacccggca cgtattccaa
    16861 aagctcctga tttctggcat ccggtaatgt ggtccaggcc gcgccttatc aagaataatc
    16921 agcctgttac cgatgacgct ctggaaatta tcggcgaaat gctgcgcttt acccagggcg
    16981 gacgttttta tagcggactg gaacagctga aaacgttttg ccagccacaa acgctggcag
    17041 cttttgcctg ggatctcttc accgcctggc aacaagctgg tgcccccgca aaagacaact
    17101 gggcatttct ggcgttaagt ctctttggtg acgaaagcac ggctcgggat ctgacgacac
    17161 agatcctcgc ctggccacaa gaaggcaaat ctgcccgtgc tgtcagtggc ctgaacatcc
    17221 ttaccctgat gaataatgat atggcgctga tacagctgca ccatatatcc caacgggcga
    17281 aatcccgctc cttacgtgat aacgcggcgg aatttcttca ggtggtcgca gaaaatcgcg
    17341 ggctaagcca ggaagagtta gcggacagat tagtcccaac actgggtctt gatgatccgc
    17401 aggcgttgat ttttgatttt ggtccacggc aatttaccgt tcgcttcgat gaaaacctca
    17461 acccggttat ctttgatcag caaaacgttc gccagaaaag cgttccccgg ttgcgcgccg
    17521 atgacgatca actgaaagcc cccgaggcac tggcccgact aaaagggcta aaaaaagatg
    17581 cgactcaggt gagcaaaaac ctgctcccgc gtcttgaaac tgccctgcgc accacccgac
    17641 gctggtcgct ggcagatttt cattctctgt ttgttaatca tccctttacc cgcctggtta
    17701 cccagcgatt aatatggggg gtttatccgg caaatgaacc gcgtcgttta ctcaacgcct
    17761 ttcgtgtggc cgcagagggg gaattctgca atgcacaaga tgagcccatt gacctgcctg
    17821 cggatgctct gattggcatt gcccacccgt tagaaatggc agcagaaatg cgcagtgaat
    17881 ttgcacagct ttttgccgat tacgaaatta tgccgccttt tcgccagttg gcgcggcgca
    17941 cggtgctgct cacacctgac gagtcagcca gtaacagcct gactcgctgg gaaggtaaat
    18001 ccgctaccgt tgggcaactt atgggaatgc gatacaaagg ctgggagtca ggctatgagg
    18061 acgcatttgt ctatgactta ggtgcgtacc ggctggtcct taagttttca cccggcttta
    18121 accactacag tgttgatagc aaagcgctaa tgagcttccg ttctcttcgg gtttaccgtg
    18181 acaataaatc tgtcactttt gccgaacttg atgtgtttga tttgagtgaa gcgttaagcg
    18241 cacccgacgt tattttccat taaaacacag cgggcagtga tgcaactgcc cgttatcgac
    18301 acgacttacc tccgggaaaa gcgatgatcg tgcaaaaaga gctggttgct atttacgatt
    18361 atgagatccc tgtacctgaa gatccgtttt ccttcagact tgagatccat aaatgctctg
    18421 aattatttac aggttccgtc tatcgactgg agcgattccg gctacatcca acatttcatc
    18481 aacgtgatcg agaagatgcc gaccactaat aaatggtgcg ttgatttata taagagatga
    18541 gtttattgat gagcggaaat tacgaggtga atcacatgaa acagtaatag caatttttaa
    18601 ttatgaacta cagaatatat t
//
LOCUS       DAZFJL010000094        18445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZFJL010000094 DAZFJL010000000
VERSION     DAZFJL010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.094"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            150..659
                     /locus_tag="ACHJ8C_003171"
     CDS             150..659
                     /locus_tag="ACHJ8C_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5552979.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            631..2559
                     /locus_tag="ACHJ8C_003172"
     CDS             631..2559
                     /locus_tag="ACHJ8C_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO5552980.1"
                     /translation="MNISEQQLNNMMAAVSVALQPLVRVVPMTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNCMGNDQVRTVNLIKSARVGYTKMLLGVVGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRNVPCLKDLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCEEAQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLMFFSARGDEIPPPRSITFHIWTAYSPFT
                     TWIQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYC
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            2543..2749
                     /locus_tag="ACHJ8C_003173"
     CDS             2543..2749
                     /locus_tag="ACHJ8C_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO5552981.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            2746..4338
                     /locus_tag="ACHJ8C_003174"
     CDS             2746..4338
                     /locus_tag="ACHJ8C_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5552982.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEEKRTFTEFIREGVGVHAFNGEIFVQPVWDTESTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESDLDTEKAFEYIAGAPQGQKDNPLINILDKFSTWYDTNSVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIASRLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            4328..5833
                     /locus_tag="ACHJ8C_003175"
     CDS             4328..5833
                     /locus_tag="ACHJ8C_003175"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO5552983.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGILLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQDMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQSGIEAGLA
                     DELVNASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVAQARAILAAAAPQQPVASTQSEADRIMACEEANGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTALFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5870..6217
                     /locus_tag="ACHJ8C_003176"
     CDS             5870..6217
                     /locus_tag="ACHJ8C_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO5552984.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EASGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            6275..7303
                     /locus_tag="ACHJ8C_003177"
     CDS             6275..7303
                     /locus_tag="ACHJ8C_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO5552985.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEIQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKN
                     NITQGSGKEWSKQDRDTFDPTHDLDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYIAEDGIEKRYLPEGMLVLG
                     NTAADGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7355..7729
                     /gene="gpFI"
                     /locus_tag="ACHJ8C_003178"
     CDS             7355..7729
                     /gene="gpFI"
                     /locus_tag="ACHJ8C_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO5552986.1"
                     /translation="MATKEQNLKRLDELALILGREPDISGSAAEIAQRVAEWEEEIQS
                     SGEDVQVGDTVIRERETAAHDVREDTSGALTRIRVLTCLHLCGIDGETGESVEIADVG
                     RVILIMSSDAKTHVDGGMAVYA"
     gene            7722..8075
                     /locus_tag="ACHJ8C_003179"
     CDS             7722..8075
                     /locus_tag="ACHJ8C_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO5552987.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGGEVQGVFD
                     DPESLGFAGSGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            8091..8624
                     /locus_tag="ACHJ8C_003180"
     CDS             8091..8624
                     /locus_tag="ACHJ8C_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5552988.1"
                     /translation="MAIKGLEQAIDNLSRVRKNAIPAASAMTINRVATTAINQSSSQV
                     ARETRVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMPGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVVGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            8621..9016
                     /locus_tag="ACHJ8C_003181"
     CDS             8621..9016
                     /locus_tag="ACHJ8C_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO5552989.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDSWMESRIYPAMTAIPALADLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            9024..9776
                     /locus_tag="ACHJ8C_003182"
     CDS             9024..9776
                     /locus_tag="ACHJ8C_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5552990.1"
                     /translation="MSTPNPLEPVKGAGTTLWVYNGQGDAYANPLSDDDWLRLAKVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VTAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            9790..10221
                     /locus_tag="ACHJ8C_003183"
     CDS             9790..10221
                     /locus_tag="ACHJ8C_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO5552991.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTASPSMNEAVMQIEQEVLTTWPADAIARAED
                     VVLRLSGMSGAVHADTDSTEVAKNNALTDDDFSAGKSSTAS"
     gene            10248..10661
                     /locus_tag="ACHJ8C_003184"
     CDS             10248..10661
                     /locus_tag="ACHJ8C_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO5552992.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRHFYRTHYFLDTQLDMHFSGLTY
                     AVLSLFFCDPNMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            10642..13221
                     /locus_tag="ACHJ8C_003185"
     CDS             10642..13221
                     /locus_tag="ACHJ8C_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5552993.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALAYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQASESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTASFRANLQGAWNDRENARLGLAAATLQSDMEKAGELAARD
                     RAERDASQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYESTLKKPKSSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRRDL
                     WKAENQYAVLKEAATKRQLSEQEKSLLTHEKETLEYKRQLAELGDKVEHQKRLNELAQ
                     QAARFEQQQSAKQAAISAKARGLTDRQAQRESEEQRLRDVYGDNPDALAKATSALKNT
                     WSAEEQLRGSWMAGLKSGWGEWAESATDSFSQVKSVATQTFDGIAQNMAAMLTGSEQN
                     WRGFTRSVLSMLTEIFLKQAMVGIVGSIGSAMGGAFGGGASASTGTAIQAAAANFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQ
                     MRRAEGISFNQNNHVVIQNDGINGQAGPQLMKAVYDMARKGAQDEIQAQMRDGGVFSG
                     GRR"
     gene            13218..13547
                     /locus_tag="ACHJ8C_003186"
     CDS             13218..13547
                     /locus_tag="ACHJ8C_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5552994.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWTPPYAWRQIKVTCAAWSSRVRMLRVEFSA
                     EFKQVVN"
     gene            13547..14245
                     /locus_tag="ACHJ8C_003187"
     CDS             13547..14245
                     /locus_tag="ACHJ8C_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5552995.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            14251..14994
                     /locus_tag="ACHJ8C_003188"
     CDS             14251..14994
                     /locus_tag="ACHJ8C_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5552996.1"
                     /translation="MTQTESAILAHARRCVPAESCGFVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMEAT
                     GFYRISLPSAQPGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSAWRHRHWHVSAFTGIYNDLAAASACM"
     gene            14991..15572
                     /locus_tag="ACHJ8C_003189"
     CDS             14991..15572
                     /locus_tag="ACHJ8C_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5552997.1"
                     /translation="MNTAAEAIRALSMQMPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRQA"
     gene            complement(15763..16290)
                     /gene="sodC"
                     /locus_tag="ACHJ8C_003190"
     CDS             complement(15763..16290)
                     /gene="sodC"
                     /locus_tag="ACHJ8C_003190"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO5552998.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
     gene            16424..>18445
                     /locus_tag="ACHJ8C_003191"
     CDS             16424..>18445
                     /locus_tag="ACHJ8C_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO5552999.1"
                     /translation="MGKGGGRAHTPVEVKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKVKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQYLASVILENLPERPFNIRMVRETADNTSDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIAQYCDQTVPDGFGGTEPRMTFNAYLSQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNCDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLW
                     VIKTGLLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRILSIDAASRTLTLD
                     REVTLPETGAATVNLINGSGKPVSVAITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGASFEPQSGTLNSVIPPAVQHLT
                     VEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGSGENSRLVTTAITADT"
BASE COUNT         4217 a         4476 c         5865 g         3887 t
ORIGIN      
        1 atccatgtcc ggtttctctt caagttaact atatgaaaaa tataaaaaca ggtcttctgt
       61 gaaccggaca tgaacaaaaa acagacatgt aaaccggaca tgaccggttt tgttgtgatt
      121 gtgaggtgag agtttttgcg aggtgaggag tggctacgca gactgaagtt gccaggcatt
      181 taagtctgac cgatcgccag cttcgcagat tgcagaaatt gccgggtgcc ccgatatcga
      241 ataagcgagg gcaactggat ctggatgcct ggcgcgattt ttacatatcg tatctgagga
      301 gaagtaaaaa cgatgtgcct gatggcgata gcgaagacga ctatgaggag aaattgctta
      361 ttgccagatg ggaactgaca gcagaacagg ctgttacaca gcagttaaaa aatgaggtgt
      421 caaaaggaaa acttattgac accgggttct gtatttttgc cctcagtaag ctggcaatgg
      481 cgttatccag tacgcttgat tccatccctt tatccatgca gcgacagttt cctgatttaa
      541 caccgcgcca tcttgaccat ctgaaaaccc ttattgctaa gggggcaaat cagtgtgcgc
      601 gggcagggga taaattaccg gatttactcg atgaatatat cagagcaaca actgaataat
      661 atgatggctg ccgtttcggt tgcgctgcag cctctggtca gggttgtacc aatgacggca
      721 gttgaatggg ctgatcaaaa ttattatctg cctaaagaat cttcatatgg tgagggagaa
      781 tggaaaacgc tgccattcca gatcgccatt atgaactgta tgggtaacga ccaggttcgc
      841 acggttaacc tgattaaatc tgcccgtgtt ggctatacaa agatgttgct gggggtggtc
      901 gggtatttta ttgagcataa atcccgaaac agtctgcttt ttcagcccac ggattctgcc
      961 gctgaagatt ttatgaagtc tcacgtggag gcgacgattc ggaacgtgcc atgcctgaaa
     1021 gacctttccc catggctggg tcgtaaacat cgtgacaata ctctcacgct gaaacgcttt
     1081 tcatcgggcg tcggtttctg gtgcctgggc ggtgcagccg ccaaaaacta ccgtgaaaaa
     1141 tccgtggacg tggtctgcta tgacgagctt tcctcgttcg agccggatgt cgaaaaagag
     1201 ggctcgccaa ccctgctggg ggataagcgt attgagggct ctgtatggcc aaaatccatt
     1261 cgcggctcga cgcctaaaat caaaggtacc tgccagatcg aaaaagcggc caacgagtcg
     1321 gcgcatttca tgcgtttcta tgtgccctgc ccgcactgtg aggaggcgca gtatctgaaa
     1381 tttggcgatg aatccacgcc ttttggcctt aaatgggaga aggacagccc cgaaagcgtt
     1441 ttctacctct gtgaacatca tggctgcgtg atccatcagt ctgagcttga ccagagtaac
     1501 gggcgctgga tctgtgaaaa cacgggtatg tggacccgtg acggcctgat gtttttcagc
     1561 gcccgggggg atgaaattcc gccgccgcgc tccatcacgt tccatatctg gacggcgtac
     1621 agtccgttca ccacctggat acagatagtc tatgactggc tggatgcact gaaagatccc
     1681 aacggcctga aaacctttgt gaacaccacg ctgggcgaga cctgggaaga ggccgtgggc
     1741 gaaaaactcg atcaccaggt actgatggat aaggtggtgc gttacacggc ggcggtgcct
     1801 gcccgggtgg tttatctgac ggcgggcatt gactcgcagc gaaaccgttt tgagatgtat
     1861 gtctggggat gggcaccggg agaggaagcc tttctggtgg ataaaatcat cattatgggc
     1921 cgtcccgatg aggaagagac gctgttacgt gtggatgcgg cgatcaacaa aaaatactgc
     1981 catgcagacg gaaccgaaat gaccatttcc cgtgtctgct gggacaccgg ggggatcgat
     2041 ggtgaaattg tctatcagag gtcaaaaaaa cacggtgttt tccgggtgct gccggtaaaa
     2101 ggcgcatctg tctatggcaa gccggtgatc accatgccga aaacccgcaa tcagcggggc
     2161 gtgtatctgt gtgaagtggg gacggacacc gcaaaagaaa ttctctatgc ccgtatgaaa
     2221 gccgatccca ctcctgcgga tgaagccacg tcgtatgcca tccgttttcc tgatgatccg
     2281 gagatttttt cgcagacaga ggcgcagcaa ctggtggcgg aagagcttgt ggagaagtgg
     2341 gaaaaaggaa agatgcgtct gctgtgggat aacaaaaagc ggcgtaacga agcgctggac
     2401 tgcctggtgt atgcctacgc ggcattacgt gtgtccgtgc aacgctggca gcttgatctg
     2461 gctgtactgg caaaatcccg ggaagaagag acgacccggc caaccctgaa agaactggca
     2521 gcgaagctgt ccggaggagt gaatggttac agtcgctgaa ctacaggcgc tacgtcaggc
     2581 gcgccttgat ttattaaccg gtaaacgggt ggtgtctgtc cagaaagatg gtcgcagaat
     2641 tgaatatacg gcggcttctc tggatgagct taaccgggcg atcaatgatg cggagtcggt
     2701 actggggaca acccgacgtc gccgtcgtcc gctgggagtg aggttatgaa acgaacgcct
     2761 gtcctgattg atgtgaacgg cgttccgctt cgtgagagtc tcagctacaa cgggggcggc
     2821 gcaggatttg gcgggcaaat ggcggagtgg ttgccaccgg cgcagagtgc cgatgcagcc
     2881 ctgctgcctg cgttgcgtct ggggaatgcc cgggcagatg atctggtgcg caataacggg
     2941 atagcggcca atgcggtggc cctgcataag gatcatattg tcgggcatat gtttctgatc
     3001 agctaccgtc cgaactggcg ctggctgggg atgcgggaga ctgcggcaaa aagttttgtc
     3061 gatgaggtgg aggcggcctg gtcggaatac gccgaaggga tgtctggcga gatcgacgtg
     3121 gaagagaaac gcacgtttac ggaatttatt cgtgaaggtg tgggcgttca tgcgtttaac
     3181 ggcgaaatct ttgtgcagcc ggtctgggat acggagagca cgcaactgtt tcgtacgcgt
     3241 tttaaagccg tgagtccgaa acgggtggac acgccaggac acggtatggg gaaccgtttt
     3301 ctgcgggccg gtgtggaggt cgatcgatat ggtcgtgccg ttgcgtacca tatctgtgag
     3361 gatgattttc cgttctctgg gagtggacga tgggaacgga tcccgcgtga acttcccacc
     3421 gggcgtccgg ccatgctgca tattttcgag ccggtggagg acgggcagac ccgtggggcc
     3481 aatcagtttt acagcgtaat ggaacggctg aagatgctcg attccctgca ggcaacacag
     3541 cttcagtcgg ccatagtgaa ggcgatgtat gcagcgacga ttgaaagtga ccttgatacc
     3601 gaaaaggcct ttgaatatat cgccggtgcg ccgcaggggc agaaggataa tccgcttatt
     3661 aatattctgg ataagttctc cacctggtat gacacgaata gcgtgacgct gggcggtgtc
     3721 aaaattccgc accttttccc cggtgatgat ctgaaacttc agaccgcgca ggattcagac
     3781 aatggatttt cggcgcttga acaggcgctg ctgcggtata tcgccgccgg tcttggcgtt
     3841 tcctacgaac agttgtcccg tgattactcg aaggtcagtt attcaagtgc ccgcgcatcc
     3901 gccaatgagt cgtggcgcta ttttatgggg cggcgaaaat ttattgcgtc ccggctggcc
     3961 acgcagatgt tttcctgctg gctggaagag gcacttcttc gggggattat tcgtccgcca
     4021 cgggcacgtt ttgattttta tcaggcgcga tcagcctggt cacgggctga gtggattgga
     4081 gccggaagaa tggccattga cgggctcaag gaggttcagg aatcagtgat gcgcattgag
     4141 gccggactga gcacgtatga gaaagagctg gcgctgatgg gcgaggatta tcaggacatt
     4201 ttccgccagc aggtcaggga atctgcagag cgggaaaaag ccggactctc acgtccggtg
     4261 tggatagcgc aggcgtatca gcagcagata gcggagagtc gcaggccgga agaggagaca
     4321 acaccacgtg agacgtaatc tttcacacat tattgccgca gcattcaatg aaccgctgct
     4381 tctggagccc gcctatgcgc gggttttctt ttgcgcgctc gggcgcgaga tgggggcagc
     4441 aagtctttcg gtaccacaac agcaggtaca gcttgatgct cccggaatgc tggctgaaac
     4501 ggacgagtac atggccggag gtaaacgacc ggcccgtgtt taccgggtgg tgaacggtat
     4561 tgcggtactg ccggtgaccg gcacgctggt gcaccggctg ggggggatgc ggccattttc
     4621 cggaatgact ggctatgacg gcattgtcgc ctgtcttcag caggcaatgg cagatagcca
     4681 ggtgcggggc atactgctgg acattgacag tccgggcggg caggccgccg gcgcgtttga
     4741 ctgcgctgac atgatttacc gcctccgtca gcagaagccg gtctgggcac tgtgcaatga
     4801 cacggcctgt tctgcagcca tgctgctggc gtcggcctgc tcccgacggc tggttaccca
     4861 gacatcccgt atcggctcca ttggcgtgat gatgagccat gtcagctatg ccggtcatct
     4921 ggcgcaggcc ggtgtggata tcacgctgat ttactcaggg gcgcacaagg tggatggcaa
     4981 tcagtttgaa gccttaccgg cagaggttcg ccaggacatg cagcagcgca ttgatgcggc
     5041 gcgccggatg tttgccgaaa aagtggcgat gtttaccggt ctgtctgttg atgccgtcac
     5101 gggaacagag gccgccgttt ttgaaggtca gtccggcatt gaggccgggc tggcggatga
     5161 attagtcaat gcgtcggatg ccatcagtgt gatggccacg gcgctgaaca gtaatgtcag
     5221 aggaggcact atgccgcaat taactgcaac ggaagccgcc gcgcaggaga accagcgagt
     5281 gatggggatc ctgacatgcc aggaagcgaa aggacgtgaa cagcttgcca cgatgctggc
     5341 aggacaacag ggcatgagcg ttgcacaggc ccgggcgatt ctggccgcgg cggcaccgca
     5401 gcagccggtg gcatccacgc agagtgaagc cgatcgcatt atggcgtgtg aagaagcgaa
     5461 cggtcgtgaa caactggcgg caacgctggc ggcgatgccg gagatgacgg tggaaaaagc
     5521 ccgcccgatc ctggctgctt caccgcaggc ggatgccgga ccctcactcc gtgatcagat
     5581 catggcactg gatgaggcaa aaggggctga ggcgcaggct gaacaactgg ctgcctgccc
     5641 gggaatgact gtggagagcg cccgggctgt gctggctgcg ggatcaggta aggcagaacc
     5701 ggtctctgca tccacaaccg ccctgtttga acgcatcatg gcgaaccatt caccggcagc
     5761 ggtacagggt ggcgtgccac agacgtcagc agacggtgat gcggacgtga aaatgctcat
     5821 ggccatgcca tgaagtcagt gctgatcatc aacaggaggt ttttacaata tggtaacgaa
     5881 aaccatcact gaacagcgtg cggaagtacg tatttttgcc ggtaatgatc cggctcatac
     5941 cgccacaggc agcagcggga tttcctcggc aacaccggca ctgacgcccc tgatgctgga
     6001 tgaagccagc gggaaactgg tggtctggga cggacagaaa gccggtagtg cagttggcat
     6061 actggtactg ccgcttgaag gcacagagac ggtactgacc tattacaagt cggggacctt
     6121 tgcgacggag gcaatccgct ggcctgaaag tgtggatgaa cacaaaaagg caaatgcctt
     6181 tgccggcagt gccctgagtc acgcggcgct gccgtaacac gttatcaggc caccgcggtg
     6241 gcctgactga tttctgaatg aaaggaactg atttatggga ttgtttacga cccgccagtt
     6301 actcggttat accgaacaaa aagtgaaatt tcgtgcgctg tttctggagc tgtttttccg
     6361 ccgtacggtg aatttccata ccgaagaggt gatgctggac aaaattaccg gaaaaacgcc
     6421 ggtggcggcc tatgtctccc cggttgttga aggaaaagtg ctgcgtcatc gtggtggtga
     6481 aacccgcgtg ttacgtccgg gctacgtcaa gccgaaacac gaatttaatt accagcaggc
     6541 ggttgagcgc cttcctggtg aagatccggc tcaactgaac gatccggctt accgccgtct
     6601 gcgtatcatt accgataacc tcaaacagga agagcacgcg attgtccagg tggaagaaat
     6661 acaggcggta aatgctgtgt tgtatggcaa atatacgatg gaaggagacc agttcgagaa
     6721 aattgaggtc gattttggca ggtcgacgaa gaataacatc actcagggta gtggtaagga
     6781 gtggtcaaaa caggatcgtg acacgttcga tcctacacat gatcttgacc tctactgcga
     6841 ccaggccagc ggtcttgtga atattgccat tatggacggt accgtctggc gtcttctgaa
     6901 tggttttaag ctgttccgcg aaaaactgga tacccgtcgc ggttcaaatt ctcaactcga
     6961 aacggcagtg aaagatctgg gcgcagtggt gtccttcaag gggtattacg gcgatctggc
     7021 cattgtggtg gcgaaaacgt cttatatagc agaagacggt atcgaaaaac gttatctgcc
     7081 ggagggcatg ctggttctgg ggaatactgc tgcagatggg atccgttgtt acggtgccat
     7141 tcaggatgcg caggcgttgt ccgaaggtgt ggtggcctct tcccgttatc cgaaacactg
     7201 gctgacggtg ggggatcccg cccgtgaatt taccatgacg cagtccgcgc cgctgatggt
     7261 gttgccggac ccggatgagt ttgtggtggt acaggtgaaa taatccgtga gcgggggcga
     7321 aatgcccccg tgtctttttt cacagggggc tgatatggca acgaaagagc aaaatctgaa
     7381 acggcttgat gaactggccc tgattctggg gcgtgagccg gatatatccg ggagtgccgc
     7441 agagatagcg cagcgggtgg cggaatggga agaggaaata cagtcatccg gcgaggatgt
     7501 tcaggttggg gatacggtga tccgggagcg ggaaaccgcg gctcatgatg ttcgtgagga
     7561 tacatccggt gcgttaacgc gcatcagagt tctgacctgc ctccatctct gtggcattga
     7621 tggtgaaaca ggggaatccg ttgagattgc ggatgttggt cgggtgattc tgattatgtc
     7681 ctcagatgca aaaacacacg ttgatggcgg aatggctgtt tatgcgtgat tttcagaatg
     7741 cctttgatgc tgcccttgcc ggggtggaca gtacgattgt tgaagtgatg gggctctgtg
     7801 cacagttcac ctcgggggca cagtgtggcg gagaagttca gggggttttt gacgatccgg
     7861 agtcgctggg atttgccggt agcggggtcc gtattgaagg aagcagcccg tcattatttg
     7921 tgcggacgga tacggttcgt gctgtgcggc gtggtgacac gctgaccatt aatggtgaga
     7981 cgttctgggt ggatcgtgtt tctccggatg acgggggcag ctgttatctc tggctcaacc
     8041 gtgggcaacc acccgccgtt aaccggcgac gataaacgca gggtgaaatt atggcgataa
     8101 aagggcttga gcaggcgatt gataatctga gccgggttcg taaaaacgcc attccggcgg
     8161 cttctgcaat gaccattaac cgcgtggcca caacggcgat taatcagtct tcatcacagg
     8221 ttgcccggga aaccagggtg agacggaaac tggttaagga acggtccaga ctgaaacggg
     8281 ccacggtcag aaatccgaat gccagaatta tcgttaaccg cggtgatctc ccggtgatta
     8341 agctggggat caggatgccg gggcgtcgtc cggacagcat actcaaagcc ggtcagcatc
     8401 gttatcagcg ggcatttatt cagcgattaa aaaatggtcg ctggcatgtc atgcagcgtg
     8461 tggtcgggaa aaaccgttac cccattgatg tggtgaaaat cccgatggcg gccccactga
     8521 aacaggcgtt tgatgagaat gttgaccgta tccggcgtga acgtctgccc ggagaactgg
     8581 catacgcgct gaaacaacaa ctgaggattg cgataaaacg atgaaacata ctgatatccg
     8641 tgctgcagtg ctggatgcac tggagctgca tgaacacggg gcgacgctgt ttgatggtcg
     8701 ccccgttgtt tttgacgaag aggattttcc cgcggtcgcg gtttatctga cggatgcaga
     8761 gtataccggt gaagagctgg atgcagatac ctggcgggcc acactgcata ttgaggtgtt
     8821 tttaccagca caggtaccgg attcggagct ggattcgtgg atggaaagcc ggatttatcc
     8881 ggcgatgact gcgatcccgg cactggcaga cctgattacc acgatggtta cgcagggcta
     8941 tgagtatcgt cgtgatgacg atatggcgtt atggagttct gcggatctga cttattccat
     9001 tacatacgag atgtgaggac gatatgtcaa caccaaatcc ccttgagccg gtaaaaggtg
     9061 ccggtaccac cctgtgggtt tataacggtc agggtgacgc ctatgcaaac ccgttgtcag
     9121 acgatgactg gctgcgactg gctaaggtga aggatctgac gccgggcgag atgacggcag
     9181 aaccctacga tgataactac ctggatgatg aagacgcgga ctggaccgcg accgggcagg
     9241 ggcagaagtc tgcaggagat accagtttta cgctggcctg gaaaccggga gaagaaggtc
     9301 agaaagggct tataggctgg tttgaaagcg gggatgtgcg ggcctataaa atccgttttc
     9361 cgaacggcac ggtggatgtg tttcgtggct gggtcagcag tatcggtaag gccgtgacgg
     9421 cgaaagaagt gatcacccgc acggtgaaag tgaccaacgt gggcaaacct tctgtagcgg
     9481 aagaacgcag caaaattacg ccggtcactg cgattaaggt gacgccgaca tccggtacgg
     9541 tggcaaaagg gaaaacaacc accctgacgg tttcttttga gccggaaagt gcaaccgaca
     9601 agacgttcag agcggtttcc gccgatccgt cgaaagccac cattagtgtg aaagatatga
     9661 caattacggt aaacggcgtg gcgacaggta aggtgcagat ccctgtggtg agcggaaatg
     9721 gtcagttcgc cgcagtggct gaagtcaccg ttactgaagc gggcgctgca gggtaaacgg
     9781 aggtaataca tgtttctgaa aacagaacaa tttgaatata acggtgtgtc cgtcacgctt
     9841 tccgaattgt ctgcgctgca gcgtattgag catcttgccc tcctgaaacg gcgtgcagaa
     9901 caggcagaat ccagcggcaa cctgcaggta agcgtggaag atctcgtcag aaccggcgcg
     9961 tttctggtgg cgatgtccct gtggcataac catccgcaga aaacggcatc accgtcaatg
    10021 aatgaggctg tgatgcagat cgaacaggag gtgctcacca cctggcctgc ggatgccatt
    10081 gcccgggcgg aagatgtggt gttgcgtctg tccgggatga gcggggctgt tcatgcggat
    10141 actgacagca ccgaagtggc gaaaaataac gcgctgactg atgatgattt ttctgcggga
    10201 aagtcttcga cggcgagctg aattttgccc tcagactggc gcgtgagatg gggaggcctg
    10261 actggcgcgc catgcttgcc gggatgacat ccaccgaata tgccgactgg cgacattttt
    10321 accgtacgca ttattttctc gatacccaac tggatatgca tttttccggg ctgacgtacg
    10381 ctgtactcag cctgtttttt tgcgatccga atatgcatcc ctctgatttc agtctgcttg
    10441 tcccccggca tgaggaagag caggtggaga ggccggatga ggacaaaatg ctgatgcaga
    10501 aagcggcagg acttgccgga ggcgtccggt tcggtgggga cggagggcgc gatattttat
    10561 cgtctgcgga tgtggcggat gtcatggtgg atgatgccgc attaatgatg gcttcagcgg
    10621 ggattccggg aggtgtgaga tatgtcccag ccggttggtg atcttgttat tgacctgagt
    10681 ctggatgcgg tccgtttcga tgagcagatg agccgggtaa ggcgtcattt ttcaggtctg
    10741 gataccgacg ccagaaaaac cgccagtgct gttgaacagg gcctgagccg ccaggcgctg
    10801 gctgcacaaa aagccgggat ttccgtcggg cagtataaag cggccatgcg aaccctgccc
    10861 gcacagttta cggatatcgc cacgcagctt gccggtggtc agaatccctg gctgatcctg
    10921 ctgcaacagg gcggtcaggt gaaggactcc ttcggcggga tgatccccat gttcagggga
    10981 cttgccggtg cgatcaccct gccgatggtc ggggtcacct cgctggcggt ggcgacaggt
    11041 gcgctggcgt acgcctggta ccagggggat tccacgcttt cagcgtttaa taaaaccctg
    11101 gttctttccg gtaatcagtc cggactgact gccgatcgca tgctgacgct ctccagagcc
    11161 ggacaggccg cagggctgac gtttaaccag gcgagtgagt cactggcagc cctggtgaat
    11221 gccggtgtgc gtggtggtga acagtttgat gccatcaacc agagtgtcgc gcgttttgct
    11281 tctgcatccg gtgtggaggt ggacaaggtt gcagaggctt tcggaaaact gaccaccgac
    11341 ccgacgtcgg gactgatggc gatggcgcgc cagttccgta acgtgacggc agagcagatt
    11401 gcgtatgttg cacagctgca gcgttccgga gacgaggccg gggcattgca ggcggcgaac
    11461 gatatcgcca cgaaaggctt tgatgagcag acccgtcgcc tgaaagaaaa catgggaaca
    11521 ctggagacct gggcggataa aacagggaag gcattcaaat cgatgtggga tgccattctg
    11581 gatatcggtc gtcctgagtc ctcagcggat atgctcgcca gtgcacagaa ggcatttgat
    11641 gaggcggata aaaaatggca gtggtaccag agccggagcc agcgccgggg aaaaaccgcc
    11701 tctttccggg ccaaccttca gggcgcatgg aatgaccggg aaaatgcccg tctggggctg
    11761 gcagcggcca cgctgcagtc ggatatggaa aaagccggtg aactggccgc cagggaccgg
    11821 gccgaacggg acgcatcaca gctgaagtat accggagagg cgcagaaggc gtatgagcgt
    11881 ctgctgacgc cgctggagaa atataccgcc cgtcaggaag aactgaataa ggccctgaaa
    11941 gacgggaaaa tcctgcaggc ggattacaac acgctgatgg cggcggcgaa aaaggattat
    12001 gaatcgacgc tgaaaaagcc gaagtcgtca ggagtcaaag tgtcagccgg tgagcgtcag
    12061 gaagaccagg cgcatgctgc cctgctggcg cttgaaaccg agctccggac gctggaaaaa
    12121 cacagcggtg cgaatgagaa aatcagccag cagcgtcgcg atttatggaa agcggaaaat
    12181 cagtatgcgg tcctgaaaga ggcagccacg aaacggcagt tatctgagca ggaaaaatcc
    12241 ctgctgaccc atgagaaaga gacgctggag tacaaacgcc agctggctga gctgggagac
    12301 aaagttgaac accagaaacg gctgaatgag ctggcacagc aggctgcgcg gtttgaacag
    12361 cagcagagtg cgaagcaggc ggcaatcagc gcaaaagccc gcggactcac cgaccgtcag
    12421 gcgcagcggg agtcggaaga gcagcgcctt cgtgacgtgt acggtgataa tccggatgcg
    12481 ctggcgaagg ccacatctgc actgaagaac acctggtctg cggaggagca gcttcgtgga
    12541 agctggatgg ccggtctgaa gtccggctgg ggcgagtggg cagaaagtgc gacggacagt
    12601 ttttcgcagg ttaaaagcgt ggccacgcag acctttgacg gtattgcaca gaatatggca
    12661 gcgatgctga ccggcagcga acagaactgg cgtggtttca cccgttctgt gctctccatg
    12721 ctgacagaga tttttctgaa gcaggccatg gtggggattg tcgggagtat tggcagcgcc
    12781 atgggtggtg ctttcggtgg tggggcgtct gcctccacgg ggacggccat tcaggctgcg
    12841 gcggcgaact tccatttcgc gaccggggga tttacgggaa cggggggcaa atacgaacct
    12901 gccggtattg tccaccgcgg ggagtttgtc ttcacgaagg aggcaaccag ccggattggc
    12961 gtcggcaacc tgtatcgtct gatgcgcggg tatgcggaag gtggttatgt gggcggtgcc
    13021 ggaagtccgg cgcagatgcg gcgggcggaa ggcattagtt ttaatcagaa caatcacgtg
    13081 gtgattcaga acgacggtat caacggacag gcagggccgc agctgatgaa agcggtgtat
    13141 gacatggccc gcaagggggc gcaggatgag attcaggcgc agatgcgtga tggcggcgtc
    13201 ttttccggag gcaggcgatg aaaacatttc gctggaaagt gaagccggat atggaggtga
    13261 actcgcagcc atcggtgcgt gaagtgcgtt ttggtgacgg gtattcgcag cgtatggcgg
    13321 cggggctgaa tgctgacctg aaaacatacc gtgtgacgct ttccgtgacc cgggaggagg
    13381 cccgacatct ggaggcattc ctggcagagc acggtggctg gaaggcgttt ctgtggacac
    13441 cgccttatgc ctggcggcag ataaaggtga cctgtgccgc ctggtcatca cgggttcgca
    13501 tgctgcgggt tgaattcagc gcggagttta agcaggtggt gaactgatgc aggatattca
    13561 cgaagaaagt ctgaacgagt cggttaaatc agagcagtca ccgcgggtgg tactctggga
    13621 aatcgacctg acggtacagg gtggtgagcg gtattttttc tgcaatgagc tgaatgaaaa
    13681 aggggaggcg gttacctggc aggggcggca atatcaggca tacccgattg acggcagtgg
    13741 ctttgagatg aacgggaagg gcagcagtgc ccgcccgtcg ctgacggtgt cgaatctgtt
    13801 cggtctggtc accggaatgg cggaggacct gcagagcctg gtgggggcca cggtggtccg
    13861 ccgccgggtg tatgcccgtt ttctggatgc ggtgaatttt gtggcgggca atccggaagc
    13921 ggacccggag caggagctga gcgaccgctg ggtggtggag cagatgtcag agctgacggc
    13981 catgacagcc tcgtttgtgc tggcaacacc gacggagacg gacggagcgc tgtttcccgg
    14041 tcgcatcatg ctggcgaaca cctgtatgtg ggattaccgg ggagatgaat gcgggtataa
    14101 cggtcctgcg gtggcggatg agttcgacaa ccccaccacg gatatccgta aggacagatg
    14161 cagcaagtgc atgcgcgggt gtgagatgcg cggcatggtg gctaattttg gcggtttcct
    14221 ttccattaac aaactttcgc agtaaatcca atgacacaga cagaatcagc gattctggcg
    14281 catgcccggc ggtgtgtgcc tgcggagtcg tgcggcttcg tggtgagaac gccggagggg
    14341 gagcggtata tcccttgtgt gaatatctct gcagagccgg aggcgtattt tcgtattgca
    14401 ccggaagact ggctgcgggc agagatgcag ggggagattg tggcactggt ccacagtcat
    14461 cccggtggtc tgccctggct gagcgaggcc gaccggcggc tgcagataaa aagtgcactg
    14521 tcctggtggc tggtctgccg gggggaaatt cataaattcc gctgtgtgcc acatctgaca
    14581 ggacggcgct ttgagcacgg ggtgacggac tgttacacgc tgttccggga tgcctaccat
    14641 ctggcgggaa ttgatatgcc ggattttgag cgtgaggatg actggtggcg caacggtcag
    14701 aacctgtacc tggacaatat ggaggcgact ggtttttaca ggatttccct gccttccgca
    14761 cagcctggcg atatcctgct gtgctgcttt ggcgcatcgg tggccaatca tgccgccata
    14821 tactgcggca acggtgagct gcttcaccat ctgcctgaac aactgagtaa acgggagagg
    14881 tattccgaaa aatggcaacg acgaacgcat tcagcctggc gtcaccgcca ctggcacgta
    14941 tctgccttca cggggattta caacgatttg gccgccgcct cagcctgtat gtgaacacgg
    15001 cagcggaagc catccgtgcc ctgtcgatgc agatgccggg attccgccgt cagatgaacg
    15061 aaggctggta ccagatacgt attcgcggtg aggacacggc accggaggcg gtgtacgccc
    15121 gtcttcacga acagctgggt gagggaacgg tcatccacat tgtgccgcga ctggccgggg
    15181 ccggaaaggg tggactgcag attgtgctgg gggcggcagc catcgtgggc tctttcttca
    15241 ctgccggggc atcaatggcg ttatggggtt cagccctggc agccggtggt ttttctgcca
    15301 ccacgatgct gttttcactg ggggccagca tgattctggg tggtgtggct cagatgctgg
    15361 ccccgaaggc aaaaacaccg gattaccgcg caacggataa cggcagacag aacacgtact
    15421 tttcctcgct ggataacatg attgcccagg ggaacccgat gccggtgcct tacggtgaaa
    15481 tgctggttgg ctcccgccgt atatcccagg acatcagtac ccgtgatgaa ggcggtgacg
    15541 ggaaggtggt ggttatcggg cggcaggcat aaaagcgaaa aaatcccgca gtgctcacgg
    15601 acaggaactg cgggagagtt acgaagattg agtgtaagga attattctta tgtcacgaca
    15661 aaaacattaa ctcagagagg gaggatgtgc cgttcttttc agggagaaag gatttatcgt
    15721 cctgaggaat aaaggtaagg ggcccgcccc ttacctgact gattattgaa tgatgccgca
    15781 ggccattctc gcaccaccac cgcccagggg ctccggatgg tcatgatggt tatcaccgcc
    15841 agcatgaagc atgagagaac gccctttaat ctcttttaat gagttcagtc tcggggccag
    15901 gaccgggtag ttcgcttttc cgtcatgcgt cacgaacagc gcagggaggt cgcccaggtg
    15961 tccatccgga gaccaggggc caagatgttt gccggtgttt ttcgggtcaa agtgaccgcc
    16021 agccgataat gctgcgaccg gttttccgtc tttcagtgcc ggggcgcaat ttcctttttc
    16081 gtgcacatga aaaccatgaa tgccttcaga cagagagtga agggctggtg tgaacagcag
    16141 accgtagggg gtctcctgaa tggttatttt tccaatgctg acttcttttc cgtcagcact
    16201 gacaaggttc attgggactt cctgttctgc tgcgtatccg catgatgctg ctgtcagcat
    16261 ggcaatggca gcaatgattt tacatttcat aaaaccctca ttaattccgt taacagactg
    16321 agcttgctgg ttacagggta acaaacagcg ttctgatgat atcgcgcaat agctgtgcaa
    16381 tatcctatca ctgcgattaa taataccaat tgagaggaac attatgggta aaggtggcgg
    16441 cagggcgcac acgccggttg aggtaaagga caatcttaag tccacgcaga tgatgagcgt
    16501 gattgatgcc attggtgaag ggccgattga aggtccggtg aaggggctgc agagtatcct
    16561 ggtgaacaaa accccgctga cggacacgga cggcaatcct gtgatacatg gtgtgacagc
    16621 ggtctggcgc gccggggagc aggagcagac accacctgaa ggctttgagt cctccggggc
    16681 ggaaaccgca ctgggcgtgg aagtgacgaa ggtaaagccg gtgacgcgca ccattacgtc
    16741 cgcgaacatt gaccgcctgc gggtcacctt cggggtgcag tcactgttgg agaccacctc
    16801 aaagggcgac cgtaatccct cttctgtccg actgctgatt cagttgcagc gtaacggtaa
    16861 ctgggtgacg gaaaaggatg tcaccattaa cggcaagacc acctcacagt acctggcgtc
    16921 ggtgattctg gagaatctgc ctgagcggcc ctttaacatc cggatggtcc gggagacagc
    16981 ggacaacacc tcggaccagc tgcagaataa gacgctctgg tcgtcataca ccgaaatcat
    17041 cgatgtgaaa cagtgctacc cgaacacggc gattgtgggg ttgcaggtgg atgcggagca
    17101 gtttggcggt cagcagatga cggtgaacta ccatatccgc ggtcgcatca tccaggtacc
    17161 gtcaaactat gacccggaaa aacgcacgta cagcggcatc tgggacggca gcctgaaacc
    17221 ggcatacagc aacaacccgg cctggtgcct gtgggacatg ctgactcacc cgcgctacgg
    17281 catgggaaaa cgtctggggg cggcagacgt ggacaaatgg gcgctgtatg ccattgcgca
    17341 gtactgcgac cagacggtcc cggatggttt cgggggcaca gagccgcgga tgacctttaa
    17401 tgcgtacctg tcacaacagc gtaaggcgtg ggacgttctc agtgatttct gctcggcgat
    17461 gcgctgtatg ccggtatgga acggccagac gctgacgttc gttcaggacc gcccgtcgga
    17521 tgtggtgtgg ccgtacacca actgcgatgt ggtggtggat gataacggcg tggggtttcg
    17581 ctacagcttc agcgccctga aggaccgcca cacggcggtg gaggtgaatt acaccgaccc
    17641 gcagaacggc tggcagacct ccacggaact ggtggaagac ccggaagcca tactgcgcta
    17701 cgggcgcaac ctgctgaaga tggatgcgtt cggctgcacc agtcgcggtc aggcccaccg
    17761 tgccgggctg tgggtgataa agaccggact gctggaaacg cagacggtgg atttcacgct
    17821 cgggtcacag gggctacgtc acacacccgg tgacattatt gaaatctgtg ataacgacta
    17881 tgccgggacc atgaccggcg gacgtatcct gtccatcgat gccgccagcc gcaccctgac
    17941 actggaccgt gaggtgaccc tgccggagac aggtgccgcc acggtgaacc tgattaacgg
    18001 cagcggtaag ccggtgagcg tggccatcac tgcacacccc gcgccggacc ggatacaggt
    18061 cagcaccctg cctgatggtg tggagacata cggtgtatgg gggctctccc tgccgtcact
    18121 gcgtcgtcgc ctgttccgct gtgtctccat ccgggaaaac acggacggca cctttgccat
    18181 cacggcggtg cagcacgtac cggaaaaaga agccatcgtg gataacgggg ccagctttga
    18241 gccgcagtca ggcaccctga acagcgttat tccaccggca gtgcagcacc tgacggtgga
    18301 ggtgagcgcg gctgacggtc agtatctggc acaggcgaaa tgggacacgc cgcgggtggt
    18361 gaagggtgtg cgcttcagtc tgcgcctgac cagtggaagc ggtgaaaaca gccgcctggt
    18421 gaccaccgcc atcaccgcag acacg
//
LOCUS       DAZFJL010000095        18049 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZFJL010000095 DAZFJL010000000
VERSION     DAZFJL010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.095"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(80..1402)
                     /gene="hipA"
                     /locus_tag="ACHJ8C_003192"
     CDS             complement(80..1402)
                     /gene="hipA"
                     /locus_tag="ACHJ8C_003192"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFO5553000.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAAEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     QIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEIL
                     SDFARMIPAALDNVKTLLPTDFPENVVTAVETNVLRLHGRLSREYGIK"
     gene            complement(1402..1668)
                     /gene="hipB"
                     /locus_tag="ACHJ8C_003193"
     CDS             complement(1402..1668)
                     /gene="hipB"
                     /locus_tag="ACHJ8C_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO5553001.1"
                     /translation="MMNFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            1976..2734
                     /gene="tam"
                     /locus_tag="ACHJ8C_003194"
     CDS             1976..2734
                     /gene="tam"
                     /locus_tag="ACHJ8C_003194"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFO5553002.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYIADLGCGPGN
                     STVLLHQRWPAARITGIDSSPAMIAEARSALPDCLFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLSPLGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(2738..3652)
                     /locus_tag="ACHJ8C_003195"
     CDS             complement(2738..3652)
                     /locus_tag="ACHJ8C_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO5553003.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(3849..5300)
                     /gene="uxaB"
                     /locus_tag="ACHJ8C_003196"
     CDS             complement(3849..5300)
                     /gene="uxaB"
                     /locus_tag="ACHJ8C_003196"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO5553004.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQYRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(5527..6945)
                     /locus_tag="ACHJ8C_003197"
     CDS             complement(5527..6945)
                     /locus_tag="ACHJ8C_003197"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO5553005.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFVLTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLLAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEVVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(7084..7443)
                     /locus_tag="ACHJ8C_003198"
     CDS             complement(7084..7443)
                     /locus_tag="ACHJ8C_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO5553006.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGEALSEEQQR
                     YIVTVIYHWLVIQMNQP"
     gene            complement(7443..8369)
                     /gene="glsB"
                     /locus_tag="ACHJ8C_003199"
     CDS             complement(7443..8369)
                     /gene="glsB"
                     /locus_tag="ACHJ8C_003199"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO5553007.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(8433..9821)
                     /gene="sad"
                     /locus_tag="ACHJ8C_003200"
     CDS             complement(8433..9821)
                     /gene="sad"
                     /locus_tag="ACHJ8C_003200"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO5553008.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGVDDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKGRI"
     gene            9922..10803
                     /locus_tag="ACHJ8C_003201"
     CDS             9922..10803
                     /locus_tag="ACHJ8C_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5553009.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILTLVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHAAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDAPDSAKQGYQ"
     gene            10881..11000
                     /gene="yneK"
                     /locus_tag="ACHJ8C_003202"
     CDS             10881..11000
                     /gene="yneK"
                     /locus_tag="ACHJ8C_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO5553010.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEEQAPFLIVIKM"
     gene            11142..12332
                     /gene="ydeA"
                     /locus_tag="ACHJ8C_003203"
     CDS             11142..12332
                     /gene="ydeA"
                     /locus_tag="ACHJ8C_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO5553011.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAHSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGSAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(12357..13022)
                     /gene="marC"
                     /locus_tag="ACHJ8C_003204"
     CDS             complement(12357..13022)
                     /gene="marC"
                     /locus_tag="ACHJ8C_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO5553012.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            13234..13668
                     /gene="marR"
                     /locus_tag="ACHJ8C_003205"
     CDS             13234..13668
                     /gene="marR"
                     /locus_tag="ACHJ8C_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO5553013.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            13689..14072
                     /gene="marA"
                     /locus_tag="ACHJ8C_003206"
     CDS             13689..14072
                     /gene="marA"
                     /locus_tag="ACHJ8C_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO5553014.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNN"
     gene            14104..14322
                     /gene="marB"
                     /locus_tag="ACHJ8C_003207"
     CDS             14104..14322
                     /gene="marB"
                     /locus_tag="ACHJ8C_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO5553015.1"
                     /translation="MKPLLSAIATALILFSAQGIAEQTTQPVVTSCGNVVVVPPSQEQ
                     APFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(14353..15252)
                     /gene="eamA"
                     /locus_tag="ACHJ8C_003208"
     CDS             complement(14353..15252)
                     /gene="eamA"
                     /locus_tag="ACHJ8C_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO5553016.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHATRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMATILSLM
                     YLALVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            15447..16634
                     /gene="ydeE"
                     /locus_tag="ACHJ8C_003209"
     CDS             15447..16634
                     /gene="ydeE"
                     /locus_tag="ACHJ8C_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO5553017.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDTALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIVVAWVLMLKGIRARPWGQP
                     ALC"
     gene            16761..16856
                     /gene="mgtS"
                     /locus_tag="ACHJ8C_003210"
     CDS             16761..16856
                     /gene="mgtS"
                     /locus_tag="ACHJ8C_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO5553018.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            16856..16960
                     /gene="mgtT"
                     /locus_tag="ACHJ8C_003211"
     CDS             16856..16960
                     /gene="mgtT"
                     /locus_tag="ACHJ8C_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO5553019.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(17075..17965)
                     /gene="dgcZ"
                     /locus_tag="ACHJ8C_003212"
     CDS             complement(17075..17965)
                     /gene="dgcZ"
                     /locus_tag="ACHJ8C_003212"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO5553020.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTVRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKATN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
BASE COUNT         4669 a         4615 c         4189 g         4576 t
ORIGIN      
        1 caacataaac caagggagaa tccagtcgtt ggcggttcat gattgtcctg ctcattaaca
       61 acgaccaaaa ccccatatct tacttaatac cgtattctcg gcttaaccga ccgtgcaacc
      121 tcaacacatt ggtttcaact gccgtcacca cgttctccgg aaaatctgtc ggtaataaag
      181 tcttcacgtt atccagtgct gccggaatca ttctggcaaa gtcactcagg atttcatgca
      241 tctgcacttc cgggaatttc agcacctttg ctgtcgccag aaaatgtcgc ggataaattt
      301 tatcgattgc cgtttttttg cctttggatg cgttaagccc cattgccagt ttgagatcgc
      361 tgatgtgtat tcccgtaccg ccaaggaccg gaaatgccga aatgatgtca taaaatggtg
      421 tgagccgata actgccgcca gcctgaataa atacggagaa gttttttgca tggccatccg
      481 ttgcgccaat caaccactgg aagacctgaa acttcataaa atcatagcga tctttcagcg
      541 cctcgctgga ccccattaaa aaagccataa tttgcgcgat accggggcct ccatctgatt
      601 catatttcac cgatgaaggt aaaccgaatg tctggcacat atcctcctgt ggcaagcgaa
      661 gtaaaaccgt tcgctcagta ttccaacgcc tgtcaaaacg ttcgaccgct aacgcgcgca
      721 cccttcccgc tttaatgatt tctgcgtccg gaacattcaa cccgagttct ttcgccagca
      781 gcaaacagta atactcatta tcaacgcttt ggctaagatc gagcgtcgca ttgggctgtc
      841 tgatttcgcc aatcggcaat ttgataatgt gcgtcgttgg cgttattccc ttcggaatgc
      901 accagtcatt gcctattctg agcagcgcag tcttctcctg tgcgccagca accgagatgc
      961 gaaagtcatt ttcttctcta atcatgccta acgggatatc tgctttataa gccgttaata
     1021 cttcttcaag tctggcttca gtaagctttt cccatgccat tatcggacac gttacggttt
     1081 catcttcggg gattaacgtc acggcaccaa cgctgtctcg ccctatttct gacaataaat
     1141 caaacggttg tctggattta gcatgataac gtttaacgat ccggtcacgt acaatcgggc
     1201 tatcgggtaa caggttatcg aagaagttaa atacggcatc agaggtgata ttccccctct
     1261 gcaatggcag cgaaagtgac aacggtctgg cataacggct ggctaaccac tcagctgcat
     1321 acttaaaggt gtgcgcgccg ttggctaact tcgttaactc gcctacccgc tggttgttca
     1381 tccaagtgac tagtttaggc atcaccactc cagatcttgc tgttctgttg cttctggcga
     1441 ggcatttttc gcgtcgcata gcgtcattga gagttcaagc gactgtaaaa tcttaaaaaa
     1501 tgtcgtgagc gaggtattgt caggattgtt ttcgaaattg gaaattgtcg cctgcttaat
     1561 gccgattttt ttcgccaact cgctctgcgt ccagccgttt tgctggcgaa ccagtttcat
     1621 tgcattcgcc aattgcgttg ggctatagat cttctgaaag ttcatcatgt catacgtcca
     1681 cagcaaattt atccgcttaa ggggatatta tcagttttat cctttagtga ggataaagtc
     1741 aatctgcaaa cacatcttat ccgctctacg ggataaacgc tgcgatatcc gcgatcgagg
     1801 ataaaaagga ggatttaaaa aagcacatcc taatgcggtg gcttctttga ctaccggaat
     1861 ctgtaatctt aatgacctgg gcgcgtaaaa aacgtctgtt caatgggttg atgccgtgag
     1921 gcgaatttat cagttttatc tacaattggg gtaacgcgct gacgggagta aaaaaatgtc
     1981 tgactggaac ccctctttat atctgcactt tgccgctgaa cgatcgcgtc cggcggtgga
     2041 gctgcttgcc agggtgccgc tggagaatat cgaatacatt gccgatcttg gctgtggccc
     2101 aggtaacagc accgtccttc tacaccaacg ttggcctgct gccaggataa cgggcatcga
     2161 ctcatctccg gcgatgattg ctgaagcgcg cagtgctttg ccagactgcc tgtttgtgga
     2221 agcagatatc cgcaactggc aaccggaaca ggcactcgat ctgatttttg ctaatgcctc
     2281 actgcaatgg ctgcccgacc actacgaatt gtttcctcat ctggtttcgt tactcagtcc
     2341 gctgggcgtg ctggcagtac agatgccaga taactggctg gagccgaccc atgtgctcat
     2401 gcgcgaagtt gcctgggaac aaaactaccc ggatcgcggg cgtgagccgc tggctggcgt
     2461 tcatgcttac tacgatattc tgagtgaagc cggatgtgag gtcgatatct ggcgaaccac
     2521 ttactatcac cagatgccgt cgcaccaggc gattatcgat tgggtgaccg ccactggatt
     2581 acgtccgtgg ttacaggatc tgaccgagag cgaacagcag cattttctta cgcgctacca
     2641 tcagatgctg gaagagcaat atcccctcca ggagaacgga cagatattgc tggcatttcc
     2701 gcgtctgttt attgttgccc gccgtacgga gtaattatca cgtcagctgg taatggcgat
     2761 cgggaagaat tttcgctgga atttctgctt catcgttcat ctgtaacagg tcaatttcaa
     2821 tagcgttgca gatggcatcc agcggtaaat cattgttttc agtaccgaag ggatcttcca
     2881 gttcttccgc cagacaatcc agcgaaataa aagtgtagga aatcagcaca gagataaaag
     2941 gcgtcatgta atgcaggtcc acgaccagcg caaacggcag catgatacaa aacagataca
     3001 cggtgcgatg caaaatcagg gtgtaggcaa agggaattgg cgtataggca atgcgctcgc
     3061 atcctgccag gaccgctgaa atatcattaa gccgatcgtt gaggctaata aacaggatat
     3121 ctgaaagttg tccattgcgt cgctgaaccg ccaaccactc ccccattatt aacaagatac
     3181 ggttagctgg agagttcgaa gccagtacac gctgaagatc ttcagtcttg agataatgag
     3241 ccagcacttc cgcctgtggc tgtttgcgta atgtcatgcg taaacagtgg gcgaaggcga
     3301 tttgcagccg ggcaaactcc cttacacttg ccgaatccgg caatgtcgtt tttacctcgc
     3361 gcagtaacga ccgcgaggca atcatcaact gcccccaaag ttttcgcgct tcaacgtaac
     3421 gggcgtaacc ggcattatta cgaaaaccaa gaaaaatggc gatggcgaca ccgagaatgc
     3481 tgaacggtgc gagggtgaat ttaatgccca aatgcgtgta ccagggcagc atgaaaataa
     3541 cagcgataga aaagagaaaa ttgagtagta agcgcgagga tatcttggat aatactgaac
     3601 cgtgccagac aaaaatacgg cgcagccagt gttgttgtgg acgaacaatc atggttatct
     3661 tcaggcgtgg aaaagtcgcc ctattaaacg tgattacgat cacattctca agacactact
     3721 tacaaattac ctacaagctt acaacattaa caggtgctgc atgtggcacc tgttaatgat
     3781 aaaggcgcat aggatgttgt aactaatata gtgaaataag aaacccggtc gaaaccgggt
     3841 tcagagtatt agcacaacgg gcgtacagct tcgcgcatcc ctttttcgag aatcgcatcc
     3901 aggtcattag caacctgctc gaccagacca ggcacttgcg tcaggtcctg ctcccagtgg
     3961 tctttttctg ccaacacaat cgctaccagt tcctgggtcc cgataacacg gtcacgatac
     4021 tggctccaga gttgctgata acgttccagc cagtgtgcat cgtcctgtac cggatatgtt
     4081 tctccattac gctcaccgcg atagaacgca atcaatgccg ctaatgcgaa agtaaggcgc
     4141 gccggaagtg tgccgtttgc cttctgccct gccagcagct gcggcaggat gcgtgtgcgg
     4201 aacttggtca taccgttgag cgcgatagac agcagttgat gcttgatgta cgggttacgg
     4261 aaacgtccgg tgactgcact ggcgaaagac tccagctcat cgcgtggcaa atccagtacc
     4321 ggaataattt cttcgtagat ggctttttca acgaatgcgc aaatttcagc atcgttcatc
     4381 gcttcaccta cggtatccag ccctgcctgg aacgccaccg gcaccagcgc ggtgtgcgca
     4441 ccgttgagga ttgccacttt gcgttcttta tacggtttaa tatcgtcaac gatcagcacg
     4501 ttgagcggat atttgtccag acgaagttca gtcgctaagg atttcggccc ctgaatcaca
     4561 aacaggtaaa agtgttcagc ggtgtcgagg aaaccatcgt ggtagcccag ttcttcttcc
     4621 agtttagcca cttcatcacg cggataaccg gtaacgatac ggtccaccag cgtagaacag
     4681 aagctgttag cctgatccag ccattgaata aatgcttctg gcagtgccca ctcttgcgca
     4741 tagcgcagca ccagctcacg caacgcgtcg ccattatagt caatcaactc acacgggatg
     4801 atgatccaac ctttatccag cgcaccgttg aaatggctaa aacgttcgaa cagcagacgg
     4861 gtcagttttg ccggataact taccgctggc gcgtcatcga atttatcgcc cgcatggtag
     4921 ctgatacccg cttcggtggt gttagagaaa acaaagcgca tttccggatt gtgagccagt
     4981 ttcaggaatt catcgtattc actgtagacg ctgatttcac gattaaccga gcgaatcagg
     5041 cgcgcgtcgc tgaccgcttc acccttctca ttcaggccac ggataatggt ggtataaaga
     5101 ccatcctgcg tgctcagtga cggcgggaat gaagtttcaa tcggacgaac aacgaccacg
     5161 ccagaattca gatcggtgtg ctcattcagg agatcgattt gccagtcaac aaaggcgcgc
     5221 aggaagttac cttcaccaaa ctgaatgata cgttctggat actgtgcacc gggaaaatcg
     5281 cgacgattta gtgttttcac aatgggttcc cttctgatta gtcatacaac ctgtttgaat
     5341 tggtacgaca ggttagcaaa ctttaatacg ccgaacccct gttttgatca actcctgatg
     5401 attaatgagc agttttatga gaaaagtgtg gcgcggatca tggtttaatc gaggaaacag
     5461 gccttttctt ggatcataaa gtggtagaac atattgcatt taattcgctc tgagtaaata
     5521 aagatgtcag acaacttcct caccgtaacg catagtgctg gtacggttgc gcccatcttt
     5581 cttcgagcga tacagacacg tatcggcttc gaccatcaat ttattaaagt catcagtcag
     5641 cgttcggtac gatgcgcaac cactaccgac gccaatactt accgtgagat aaagcgtttt
     5701 ttgttgccag gtgaacggtt gaagttcaac gcctttacgg attttttccg ccattagcag
     5761 gccatcgaca ggattcaccg acggcactgc aacagcaaat tcttcgccgc ccattcgcgc
     5821 caccagcccc ttatcaccga caatcttctg aatatgctgg gcaaacacgc ttaacacttt
     5881 atcgccacat tcatgtccat agttatcgtt aatgcttttg aaatagtcga tatcaagcag
     5941 catgaccgtc agatgttgtg tctgtttcag cgatggactt ttcagcgcct cataaagacc
     6001 ggaccgtgag taaacctgag tcagaaaatc aaagtcggct cgcagcgcaa cttgcttcat
     6061 tagcgaattg atcgctgcca cgctaaagga aaccataatt gggcatatcg ccatcgtggc
     6121 aataccgaga cgtgcggaga acatttgcgg aatggagaac ggcgaaccga ccgagatatc
     6181 aatcaccgaa tttgccacca gtacgacttc caccgcaccg gtgacaaagg tcaacagaca
     6241 tgttacctgc ggcgtatagc gcactgcaca ccagattaat gcagggagcg gaaacgccag
     6301 actccccgct ccgccaatga ctaccgaggc aataaccgac acaataagcg cgatagcagg
     6361 catcatctgc tctgctttaa agcgcggcag tactccagga attgccaacg tcagcataca
     6421 aggcacgatc aacacgcccg ttgagaattg ctcactaaac cagtcggcaa gcaaaggcca
     6481 gaaatccaga ctatcaatac tgaccgaacc aatcgccccg acaatagcgc ataataatgc
     6541 ggcaagcaga cagtaattaa atagccgtaa ggcacttact ggctcatact tattttttcc
     6601 cagacgctta tcccgcacga ccagtaaggc gacggtaaca ataaacatca tattggagaa
     6661 attgatagcc agtgaaacca gcccccattc ggtggttatg gcatcataaa ccagcatcgc
     6721 cacataactg atcgcatagt aatgcagtcg attaagccag acatagcggg caaacacccc
     6781 cgccattacg ccattcagcg gccagaaaag agaaagttcg tgaaccagcc gtaactctgc
     6841 accaataaaa tagaacagcg tggttagcac aaaaatagcg atgctattac gtagcagatg
     6901 accttcctgg aacaaacgga aagttgaaat gggctgtaca tgcattaatt atctgtccat
     6961 acaattgcgg gtggcaatta tctcaacgca ataagtaaat tcttagaaag gatagcatgt
     7021 tcgctgacaa cggcggcaac taaagatacc taatgaatgc cattttatcg atttccgtaa
     7081 ttatcatggc tgattcatct gtataaccag ccagtgatag attaccgtca caatataacg
     7141 ttgttgctcc tcacttaacg cttccccctg aggaatgtta tgccacttag ccagacaacc
     7201 gcgacaacag gtcgcggtgg cgtgttgagc gataaaaacc ggatgcccgc gcatgggggt
     7261 ttgcttaccg tcgttagccg gtaatgccgg agcaagacgt ttagcgacaa aatccgccgc
     7321 atgttgttca atgactggcg cgcctttctc cagacaatac tgacgctctt tcacacccaa
     7381 acgaaagcga gagcgaaatt ttgaacggga taaacgcgcg aagagtggat cgagagactg
     7441 cattaataaa ctgaacgccc taattgtttc gtcaattgtt caaggacggc aatgcccgca
     7501 agcgagttac cggcatcatc cagttccgga ctccagactg cgatggccat ttcatgcgga
     7561 acaatcgcca caataccgcc accaacgcca gattttgccg gtagtcccac ccgccaggca
     7621 aactcccccg cgttctggta cataccgctg gtcgccatca gcgcgttaat ttgccgcgcc
     7681 tgcattggcg tcaccacagg ttcatcaata tgaatagctt tcccctgatt cgccagaaag
     7741 acaaacgtcc gggccagctc tacacagctc attttcagag cgcagtaatg aaagtagttt
     7801 tgcagaacgg ttgtcacgtc atgatggaaa ttgccaaacg acttcatcag ccaggcgata
     7861 gccgcatttc gcgcggaatg ttcaaattcg gaacgcgcta ccaccgtatc gtaggaaata
     7921 tcagacacac cacttaagcc gcgcacgact tccagcatac gttgccgagg agcgcttaat
     7981 cgcccttgca acatatcgca gaccaccagc gcaccggcat taataaacgg attacgcgga
     8041 ataccctgct ccatttccag ttgcactaaa gaattgaacg gtgatccaga cggatctttg
     8101 ccgacgcgtt gccagatttc ctcttcggag taatgacgca tggcgacaac gagactcagc
     8161 actttggaaa tagactgaat ggaaaaacgt tcttgcgcgt ctccggcctg aaaaagctgt
     8221 ccgtcaacgg tacagatagc aatccccaat cgggaaccgt ctaccgtagc cagcgccgga
     8281 atataatccg cgactttacc ctgaccaatg agcggccgca cttgccgcaa gatgttttct
     8341 aaaattgcat tatccatggc gactgccact ttctactcct ggaccgcagg tctgaaaaga
     8401 cctgcgagta tatcagagct gaatatgtag cgtcagatcc ggcctttcca caccgtctgg
     8461 atattacaga attcgtgtaa gccaaaatgg gaaagctcac gcccaaagcc actcttcttc
     8521 acgccaccaa aggcgactcg cgcatcgctg gcacaataac cattgataaa caccccaccg
     8581 cattccagac gtgccgccat ctgtctggcc tgtgtttcgt cggtggtgaa aatggtcgct
     8641 gaaaggccga actcactatc attagccagt tccagcgcat gttctgcatc tttcgcaatg
     8701 gtgattgccg caacggggcc aaacatttct tcccgaaacg cggtcatttc tggggtaaca
     8761 ttcgccagaa ccgttggcgg atagtagtta cctgccccag ccatcttttc cccgcccagt
     8821 aacaaacgcg caccctgcgc cagtgttttt tccacctgat gatgcagctc atcacgtaaa
     8881 tcaaaacgag ccattggtcc gagagcgttc tcttcatcac ggggatcgcc cattttcaag
     8941 gctgccgcag ctgccacaaa acgttcggta aatgccgaag caattccctc ttcgatgata
     9001 aagcgttttg ccgctgcaca aacctgtccg gtattctgat aacgtccggc taccgccgct
     9061 ttcaccgcca gttccagatc ggcatcgtta agcacaataa acggatccga accacccagt
     9121 tccagtacgc attttttcag tgccgctcca gcctgtgcgc caatagccgc tccagcacga
     9181 acacttccgg tcactgtgac agcagcaatg cgcgagtctt taatcatctg actgacaccg
     9241 tcgttgtcgg cattcagcca gccatatacg ccttgtggga tacccgcatc tttaaacacc
     9301 tgggcaatga gctgtgcaca gcccatcaca ttcggcgcat gtttaagtaa gtagccgtta
     9361 ccagcaagaa tgatgggaac agcgccacgc atcacctgcc ataacggaaa attccacggc
     9421 ataatcgcca gaatcgtccc caacggtcga tactcaataa ccgcctgctg attttccacc
     9481 agcgtaggtt ctgccttcag cattgccgga ccatgttctg cataccagtc acacaaattc
     9541 gccgatttcg ccacttcagc gcgcgcctgg ttgattggtt tgcccatttc acgggtgatc
     9601 atttgcgcca tttcttcgct acgggcgcgc agagccttac cgatatcacg cagtttttca
     9661 gcacgataat ctatatttgt ctcgcgccag tcgcgaaagc ctgctgccgc cagctgaagc
     9721 gcgttttcga tatcgtcaac accagcccac ggcagcacag aaagttgttc accggtggca
     9781 ggatttatcg aaattgcatg agttgccgga gtaatggtca tcggggtatc tcctttatga
     9841 gtcatggtat gaagatacgc agatttactc ttgctttaaa atgaataata ttaagccact
     9901 tattcacgaa tcgagaatgt tatggatctg acccaactgg agatgttcaa cgcggttgcc
     9961 gaagctggca gcattaccca ggctgcagca aaagtgcatc gcgtgccgtc gaatctcact
    10021 acccgtctac gtcagctgga aacagaacta ggggttgatc tgtttattcg cgagaatcag
    10081 cgtttacgtc tctcgcctgc cgggcataac tttttacgct atagccaaca aattctcacg
    10141 ttagtggatg aagcgcggag cgttgtcgct ggcgatgagc cgcaaggttt attttctctg
    10201 ggatcgctgg aaagcaccgc tgcagtgcgc attccggcca cgctggcgga atttaaccgt
    10261 cgttatccca aaattcagtt ttcactttcc accggccctt ccggcactat gctggaaggt
    10321 gtgctggagg gaaaactgaa tgcggcgttt attgatggac ccattaacca tgctgccatc
    10381 gacgggatac cggtataccg cgaggaacta atgatcgtca cgccacaagg acatgcgcca
    10441 gtaacccgtg ccagtcaggt gaatggcagt aacatttatg ccttccgcgc caattgttcg
    10501 tatcgtcgcc acttcgaaag ctggtttcat gctgacggtg ccgctccggg aactattcac
    10561 gagatggagt cttatcacgg gatgttggcc tgtgtggtcg caggagcagg cattgcgctt
    10621 attccacgct ctatgctgga aagtatgccg gggcatcacc aggttgaagc gtggccgtta
    10681 gctgagcaat ggcgttggtt aacaacctgg ctggtctggc gtcgtggtgc gaaaacgcgt
    10741 ccgcttgagg catttattca actgctggat gcgcctgact cggcaaaaca gggttaccaa
    10801 tgagctattt ttgatagttc atgcggctaa tatgctctat atagtgatgt tccgatgact
    10861 tatgactata tggggcaaat atggttacgc cagtaagcat cagcaattac atatctcttc
    10921 ctgatgattt tcccgttcgt aatattgcac ctcaagtaaa agaagaacag gcccccttcc
    10981 tgatcgtcat taaaatgtga tgttaacaac tctctgatgc gtgacagaac aaccgtcaga
    11041 gagatggaaa tgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag
    11101 atcatttcat cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt
    11161 cccgcaaagt agcgtggcta cgggtcgtta cgctggcagt cgccgccttt atcttcaata
    11221 ccaccgaatt tgtccctgtt ggcctgctct ctgacattgc gcatagtttt cacatgcaaa
    11281 ccgctcaggt cggcatcatg ttgacaattt atgcatgggt agtggcgcta atgtcattgc
    11341 cttttatgtt aatgaccagc caggttgaac gccgcaaatt actgatctgc ctgtttgtgg
    11401 tgtttattgc cagccacgta ctgtcgtttt tatcgtggag ttttaccgtt ctggtgatca
    11461 gtcgcattgg tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga
    11521 tccgtatggc tccggccggg aagcgagcac aggcattgag tttaattgcc accggtacag
    11581 cactggcaat ggtgttaggt ttacctctcg ggcgcattgt ggggcagtat tttggctggc
    11641 gaatgacctt cttcgcgatt ggtatcgggg cgcttattac ccttttgtgc ctgattaagt
    11701 tacttccctt gctgcccagt gagcattccg gttcattgaa aagcctcccg ctattgttcc
    11761 gccgcccggc attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca
    11821 cggcatacag ctatatcgag ccttttgtac aaaacattgc gggattcagc gccaactttg
    11881 ccacggcatt actgttatta ctcggtagtg cgggcattat tggcagcgtg attttcggta
    11941 aactgggtaa tcagtatgcg tctgcgttgg tgagtacagc gattgcgcta ttgctggtgt
    12001 gcctggcact gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt
    12061 tctgggggat cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg
    12121 caccagatgc caccgacgtc gcgatggcgc tattctccgg gatatttaat attggaatcg
    12181 gcgcgggtgc gttggtaggt aatcaggtga gtctgcactg gtcaatgtcg atgattggtt
    12241 atgtaggcgc ggtgcctgct tttgctgcgt taatatggtc aatcatcatt tttcgccgct
    12301 ggccagtgac actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa
    12361 tgatacgttt taatgatttc caggatgccg ttaataataa actgcacgcc catacatacc
    12421 agcaggaatc ccatcagacg ggagatcgct tcaataccac ccttgcccac cagccgcata
    12481 attgcgccag agctgcgtag acttccccac aaaataaccg ccaccaggaa aaagatcagc
    12541 ggcggcgcaa ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggaggcc
    12601 gagctaataa tcatcgcaat ggttcccgga ccggcggtac ttggcattgc cagtggtaca
    12661 aaagcaatat tggcactggg ttcatcttcc agctcttccg acttgctttt cgcttccggt
    12721 gaatcaatcg ctttctgttg cggaaagagc atccgaaaac cgataaacgc gacgattaag
    12781 ccgcctgcaa ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgcccg
    12841 gcgtaatacg ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga
    12901 ttacgttcgg cactgttcat gttgcctgcc aggccaagaa ataacgcgac agttgttaat
    12961 gggttagcta acggcagtaa caccaccagc cccaggccta ttgctttaaa caaatctaac
    13021 attggtggtt gttatcctgt gtatctggtt tatcagcgaa aagtataagg ggtaaacaag
    13081 gataaagtgt cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta
    13141 gcaaaacgtg gcatcggtca attcattcat ttgacttata cttgcctggg caatattatc
    13201 ccctgcaact aattacttgc cagggcaact aatgtgaaaa gtaccagcga tctgttcaat
    13261 gaaattattc cattgggtcg cttaatccat atggttaatc agaagaaaga tcgcctgctt
    13321 aacgagtatc tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc
    13381 cgctgcgcgg cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctgggg
    13441 gcactgaccc gtatgctgga tcgcctggtc tgtaaaggct gggtggaaag gttgccgaac
    13501 ccgaatgata agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgtgaa
    13561 caatgccatc aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg
    13621 gacgaagtgg caacacttga gcatttgctt aagaaagtcc tgccgtaaac gaaaaaagag
    13681 gtatgacgat gtccagacgc aatactgacg ctattaccat tcatagcatt ttggactgga
    13741 tcgaggacaa cctggaatcg ccactgtcac tggagaaagt gtcagagcgt tcgggttact
    13801 ccaaatggca cctgcaacgg atgtttaaaa aagaaaccgg tcattcatta ggccaataca
    13861 tccgtagccg taagatgacg gaaatcgcgc aaaagctgaa ggaaagtaac gagccgatac
    13921 tctatctggc agaacgatat ggcttcgagt cccaacaaac tctgacccga accttcaaaa
    13981 attacttcga tgttccgccg cataaatacc ggatgaccaa tatgcaaggt gaatcgcgtt
    14041 ttttacatcc attaaatcat tacaacaact agttgataac gtgacaacgt cactgaggca
    14101 atcatgaaac cacttttatc cgcaatagca actgcgctta ttctcttttc tgcgcagggc
    14161 attgcggaac aaaccacgca gccggttgtt acttcctgtg gcaatgtcgt ggttgttccc
    14221 ccatcgcagg aacaagcacc gtttgattta aatcacatgg gtactggcag tgataagtcg
    14281 gatgcgctcg gcgtgcccta ttataatcaa cacgctatgt agtttgttct ggccccgaca
    14341 tctcggggct tattaacttc ccacttttac cgctttacgc caccgcaagc caaatacatt
    14401 gatatacagc ccggtcataa tgagcaccgc accgaaaaat tgcagacccg ttaagcgttc
    14461 atccaacaat agtgccgcac ttgccagtcc aactacaggc accagtaacg ataacggtgc
    14521 aacccgccag gtttcatagc gtcccagtaa cgtcccccag atcccataac caacaattgt
    14581 cgccacaagc gccagataca tcagagacaa gatggtggcc atatcgatag taaccagact
    14641 gtgaatcatg gttgcggaac catcgagaat cagcgaggca acaaagaagg gaatgattgg
    14701 gattaatgcg ctccagatta ccagcgacat cactgccgga cgcgttgcgt gcgacatgat
    14761 ctttttattg aagatgttgc cacacgccca actaaatgct gccgccaggg tcaacataaa
    14821 gccgagcatc gccacatgct gaccgttcag actatcttcg attaacacca gtacgccaaa
    14881 aatcgctaag gcgatccccg ccaattgttt gccatgcagt cgctcaccga aagtaaacgc
    14941 gccaagcacg atagtaaaaa acgcctgtgc ctgtaacacc agcgaagcca gtccagcagg
    15001 cataccaaag ttaatggcgc agaacagaaa ggcaaactgc gcaaaactga tggttaatcc
    15061 ataccccaac agcaaattca gtggtacttt cggtcgtgcg acaaaaaaga tggccgggaa
    15121 agcgaccagc ataaagcgca aaccggccag catcagcggt ggcatgttat gaagccccac
    15181 tttgatgacc acaaaattta gcccccatac aaccactacc agtagcgcca acaccccatc
    15241 ttttcgcgac attctaccgc ctctgaattt catcttttgt aagcaattaa cttagctgaa
    15301 tttacttttc tttaacagtt gattcgttgg tcaccgttta cgacggaatt aatgcgcaaa
    15361 taagtcgcta tacttcggat ttttgccatg ctattccttt acatctctaa aacaaaacat
    15421 aacgaaacgc actgccggac agacaaatga acttatccct acgacgctct accagcgccc
    15481 ttcttgcctc gtcgttgtta ttaaccatcg gacgcggcgc tacgctgccg tttatgacca
    15541 tttacttgag tcgccagtac agcctgagtg tcgatctaat cggttatgcg atgacaattg
    15601 cgctcactat tggcgtcgtt tttagcctcg gttttggtat cctggcggat aagttcgaca
    15661 agaaacgcta tatgttactg gcaattaccg ccttcgctag cggttttatt gccattcctt
    15721 tagtgaataa cgtgacgctg gtggtgctct tttttgccct tattaactgc gcctactccg
    15781 tttttgctac cgtgctgaaa gcctggtttg ccgacaatct ttcgtccacc agcaaaacga
    15841 agattttctc aatcaactac accatgctca acattggctg gaccatcggt ccgccgctcg
    15901 gcacgctgtt ggtgatgcag agcatcaatc tgcccttctg gctggcagct atctgttccg
    15961 cgtttcccat gcttttcatt caaatttggg taaagcgcag cgagaaaatc atcgccacgg
    16021 aaacaggcag tgtctggtcg ccgaaagttt tattacaaga tacagcactg ttgtggttta
    16081 cctgctctgg ctttctggct tcttttgtta gcggcgcatt tgcttcatgc atttcacaat
    16141 atgtgatggt gattgctgat ggtgattttg ccgaaaaggt ggtcgcggtt gttcttccgg
    16201 tgaatgctgc catggtggtt acgttgcaat attccgtggg ccgccgcctt aacccggcta
    16261 acatccgcgc gctgatgaca gcaggcaccc tctgtttcgt catcggtctg gtcggtttta
    16321 ttttttccgg caacagcctg ctattgtggg gtatgtcagc tgcggtattt actgtcggtg
    16381 aaatcattta tgcgccgggc gagtatatgt tgattgacca tattgcgccg ccaggaatga
    16441 aagccagcta tttttccgcc cagtctttag gctggcttgg tgctgcgatt aacccattag
    16501 tgagtggcat agtacttacc agtctgccgc ctttctcgct gtttatcatc ttagcgttag
    16561 tgatcgttgt tgcgtgggtg ctgatgttaa aaggaattcg tgcaagaccg tgggggcagc
    16621 ccgcgctttg ttgatttaag tcgaacacaa taaaaattta attcagcctt cgtttaggtt
    16681 acctctgcta atatctttct cattgagatg aaaattaagg taagcgagga aacacaccac
    16741 accataaacg gaggcaaata atgctgggta atatgaatgt ttttatggcc gtactgggaa
    16801 taattttatt ttctgggttt ctggccgcgt atttcagcca caaatgggat gactaatgaa
    16861 cggagataat ccctcaccta accggcccct tgttacagtt gtgtacaagg ggcctgattt
    16921 ttatgacggc gaaaaaaaac cgccagtaaa ccggcggtga atgcttgcat ggatagattt
    16981 gtgttttgct tttacgctaa caggcatttt cctgcactga taacggatcg ttgacacagt
    17041 agcatcagtt ttctcaatga atgttaaacg gagcttaaac tcggttaatc acattttgtt
    17101 cgtcaataaa catgcagcga ttccttccgg tttgcttacc ctcatacatt gccctgtccg
    17161 ctcttccaat gaccacatcc agaggctctt caggaaatgc acgactcaca ccagcagtca
    17221 cggtaatgtt gatatgccct tcagaatgtg tgatggcatg gttatcgact aactggcaaa
    17281 ttctgacacc tgcacgacat gcttcttcat cattagtcgc tttgacaata atgataaatt
    17341 cttcgccccc gtagcgataa accgtttcgt aatcgcgcgt ccaactggct aagtaagttg
    17401 cgagggtgcg taatactaca tcgccgatta aatgcccgta ggtatcatta accaatttaa
    17461 atcggtctat atccaacaac attaaataaa gattcagagg ttcagcattg cgtaactgat
    17521 gatcgaagga ttcatcaaga acccgacgac ccggcaatcc cgtcagaaca tccatattgc
    17581 tacggaccgt aagcaaataa attttgtaat cggttaatgc cgcagtaaaa gaaagcaacc
    17641 cttcctgaaa agcgtcgaaa tgcgcgtcct gccagtgatt ttcaacaata gccagcatta
    17701 attcccgacc acagttatgc atatgttgat gggcagaatc cattagccga acgtaaggta
    17761 attcatcgtt atcgagtggc cccagatgat caatccaccg accaaactgg cacagtccat
    17821 aagaatggtt atccgttatt tctggcttac tggcatctct cgcgaccacg ctgtgaaaca
    17881 tacttaccag ccactggtag tgggcatcga tagccttatt gagatttaac aagatggcat
    17941 caatttccgt tgtcttcttg atcattgcca ctcctttttc acagttcctt gtgcgcgcta
    18001 ttctaacgag agaaaagcaa aattacgtca atattttcat agaaatccg
//
LOCUS       DAZFJL010000096        17830 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZFJL010000096 DAZFJL010000000
VERSION     DAZFJL010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.096"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            104..178
                     /gene="tnaC"
                     /locus_tag="ACHJ8C_003213"
     CDS             104..178
                     /gene="tnaC"
                     /locus_tag="ACHJ8C_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO5553021.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            399..1814
                     /gene="tnaA"
                     /locus_tag="ACHJ8C_003214"
     CDS             399..1814
                     /gene="tnaA"
                     /locus_tag="ACHJ8C_003214"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO5553022.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1905..3152
                     /gene="tnaB"
                     /locus_tag="ACHJ8C_003215"
     CDS             1905..3152
                     /gene="tnaB"
                     /locus_tag="ACHJ8C_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO5553023.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     IIIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDSTSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHAGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3285..4460
                     /gene="mdtL"
                     /locus_tag="ACHJ8C_003216"
     CDS             3285..4460
                     /gene="mdtL"
                     /locus_tag="ACHJ8C_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO5553024.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSALFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIAVLMLSLFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4435..5394
                     /gene="yidZ"
                     /locus_tag="ACHJ8C_003217"
     CDS             4435..5394
                     /gene="yidZ"
                     /locus_tag="ACHJ8C_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO5553025.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSRNPKIVWLRETIKNLYASMT"
     gene            5551..6300
                     /gene="yieE"
                     /locus_tag="ACHJ8C_003218"
     CDS             5551..6300
                     /gene="yieE"
                     /locus_tag="ACHJ8C_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO5553026.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            6322..6888
                     /gene="yieF"
                     /locus_tag="ACHJ8C_003219"
                     /gene_synonym="chrR"
     CDS             6322..6888
                     /gene="yieF"
                     /locus_tag="ACHJ8C_003219"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO5553027.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEINALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQSTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6942..8279)
                     /gene="adeP"
                     /locus_tag="ACHJ8C_003220"
     CDS             complement(6942..8279)
                     /gene="adeP"
                     /locus_tag="ACHJ8C_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO5553028.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMSVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8447..9112
                     /gene="yieH"
                     /locus_tag="ACHJ8C_003221"
     CDS             8447..9112
                     /gene="yieH"
                     /locus_tag="ACHJ8C_003221"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO5553029.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9179..9652
                     /locus_tag="ACHJ8C_003222"
     CDS             9179..9652
                     /locus_tag="ACHJ8C_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553030.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLILLVIIQ
                     VLPLFLLLSITTGAIPAMLTGVMVACLPEKIGSQKRYRCLVGGIGGVVITEIYCAVII
                     HIKDMASSALFENILSGENLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            complement(9734..10456)
                     /gene="yieK"
                     /locus_tag="ACHJ8C_003223"
     CDS             complement(9734..10456)
                     /gene="yieK"
                     /locus_tag="ACHJ8C_003223"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO5553031.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKMRRVNLAITAGSTPKGMY
                     EYLITLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGVDGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLIVIADKAAAAELALG"
     gene            complement(10471..11640)
                     /gene="yieL"
                     /locus_tag="ACHJ8C_003224"
     CDS             complement(10471..11640)
                     /gene="yieL"
                     /locus_tag="ACHJ8C_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFO5553032.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     TDNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPVLKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVVHGDLIAITYHSNALQSERQMY
                     VWTPPGYPGIGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNEPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(11667..13283)
                     /gene="bglH"
                     /locus_tag="ACHJ8C_003225"
     CDS             complement(11667..13283)
                     /gene="bglH"
                     /locus_tag="ACHJ8C_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFO5553033.1"
                     /translation="MFRRNIITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSRAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAIVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDRATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKYNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQTEIWW"
     gene            complement(13369..14763)
                     /gene="bglB"
                     /locus_tag="ACHJ8C_003226"
     CDS             complement(13369..14763)
                     /gene="bglB"
                     /locus_tag="ACHJ8C_003226"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFO5553034.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVVLWLTFNEINMSLHAPFTGVGLAEESCEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRILLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRITYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(14782..16656)
                     /gene="bglF"
                     /locus_tag="ACHJ8C_003227"
     CDS             complement(14782..16656)
                     /gene="bglF"
                     /locus_tag="ACHJ8C_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFO5553035.1"
                     /translation="MTELARTIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKVDALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPITFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLIPLIINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPGIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQSAQVAQQEKTPEVITPEQGGICSPMTGDIVPLIHVADTTFASGLLGKGIAI
                     LPSVGEVRSPVAGQIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVG
                     DKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQINAGEPLLSIIR
                     "
     gene            complement(16790..17626)
                     /gene="bglG"
                     /locus_tag="ACHJ8C_003228"
     CDS             complement(16790..17626)
                     /gene="bglG"
                     /locus_tag="ACHJ8C_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFO5553036.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRPGERINSS
                     GIEKEYALSSHELNGRLSELLSHMPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         4548 a         4464 c         4142 g         4676 t
ORIGIN      
        1 gtcccgaacg attgtgattc gattcacatt taaacaattt cagaatagac aaattccttg
       61 agtattatac tgtagcctcg tgtctttcga ggataagtgc attatgaata tcttacatat
      121 atgtgtgacc tcaaaatggt ttaatattga caacaagatt gtcgatcacc gcccttgatt
      181 tgcccttctg tagccatcac cagagccaaa ccgattagat tcaatgtgat ctatttgttt
      241 gctatatctt aattttgcct tttacaaagg ccatctctcg tttatttact tgttttagta
      301 aatgatggtg cttgcatata tatctggcga attaatggat actgcaaatg taatattcac
      361 agggatcact gtaattaaaa taaatgaagg attatgtaat ggaaaacttt aaacatctcc
      421 ctgaaccgtt ccgcattcgt gttattgagc cagtaaaacg taccactcgc gcttatcgtg
      481 aagaggcaat tattaaatcc ggtatgaacc cgttcctgct ggatagcgaa gatgtgttta
      541 tcgatttact gaccgacagc ggcaccgggg cggtgacgca gagtatgcag gccgcgatga
      601 tgcgcggcga cgaagcttac agcggcagcc gcagctacta cgcgttagcc gagtcagtga
      661 aaaatatctt tggttatcaa tacactattc cgactcacca gggccgtggt gcagaacaaa
      721 tctatattcc ggtactgatt aaaaaacgcg agcaggaaaa aggcctggat cgcagcaaaa
      781 tggtggcatt ctctaactat ttctttgata ccacgcaggg ccatagccag attaacggct
      841 gtaccgtgcg taacgtctat atcaaagaag ccttcgatac tggcgtgcgt tacgacttta
      901 aaggcaactt tgaccttgag ggattagaac gcggtattga agaagttggc ccgaataacg
      961 tgccgtatat cgttgcaacc atcaccagta actccgcagg tggtcagccg gtttcactgg
     1021 caaacttaaa agcgatgtac agcatcgcga agaaatacga tattccggtg gtcatggact
     1081 ccgcacgctt tgccgaaaac gcctatttca tcaaacagcg tgaagcagaa tacaaagact
     1141 ggaccatcga gcagatcacc cgcgaaacct acaaatatgc cgatatgctg gcgatgtccg
     1201 ccaagaaaga tgcgatggtg ccgatgggcg gcctgctgtg catgaaagac gacagcttct
     1261 ttgatgtgta taccgaatgc agaacccttt gcgtggtgca ggaaggcttc ccgacatatg
     1321 gcggcctgga aggcggcgct atggagcgtc tggcggtagg tctgtatgac ggcatgaatc
     1381 tggactggct ggcttatcgt atcgcgcagg tgcagtatct ggtcgatggt ctggaagaga
     1441 ttggcgttgt ctgtcagcag gcgggcggtc acgcggcatt cgttgatgcc ggtaaactgc
     1501 tgccgcatat cccggcagat cagttcccgg cacaggcgct ggcctgcgag ttgtataaag
     1561 tcgccggtat ccgtgcggta gaaattggct ctttcctgtt aggccgcgat ccgaaaaccg
     1621 gtaaacaact accatgcccg gctgaactgc tgcgtttaac cattccgcgc gcaacatata
     1681 ctcaaacaca tatggacttc attattgaag ccttcaaaca tgtgaaagag aacgcggcga
     1741 atattaaagg gttaaccttt acctacgaac cgaaagtatt gcgtcacttc accgcaaaac
     1801 tgaaagaagt ttaattaata ctacagagtg gctataagga tgttagccac tctattaccc
     1861 tacatcctca ataacaaaaa tagccttcct ctaaaggtgg catcatgact gatcaagctg
     1921 aaaaaaagca ctctgcattt tggggtgtta tggttatagc aggtacagta attggtggag
     1981 gtatgtttgc tttacctgtt gatcttgccg gtgcctggtt tttctggggt gcctttatca
     2041 ttatcattgc ctggttttca atgcttcatt ccgggttatt gttattagaa gcaaatttaa
     2101 attatcctgt cggctccagt tttaacacca tcaccaaaga tttaatcggt aacacctgga
     2161 acattatcag cggtattacc gttgccttcg ttctctatat cctcacttat gcctatatct
     2221 ctgctaatgg tgcgatcatt agtgaaacga tatcaatgaa tttgggctat cacgctaatc
     2281 cacgtattgt cgggatctgc actgccattt tcgttgccag cgtattgtgg ataagttcgt
     2341 tagccgccag tcgtattacc tcattgttcc ttgggctgaa gattatctcc tttgtaattg
     2401 tttttggttc tttcttcttc caggtcgatt actccattct gcgcgattcc accagcacca
     2461 ctgcggggac gtcttacttc ccgtatatct ttatggcttt gccggtgtgt ctggcgtcat
     2521 ttggtttcca cggcaatatt cccagcctga ttatttgcta tggaaaacgc aaagataagc
     2581 tcatcaaaag cgtggtcttc ggttcgctgc tggcgctggt gatttatctc ttctggctct
     2641 attgcacgat ggggaatatc ccgcgagaaa gctttaaggc gataatctcc tcaggcggca
     2701 acgttgattc gctggtgaaa tcgttcctcg gcaccaaaca gcacggcatt atcgagtttt
     2761 gcctgctggt gttctccaac ttagctgttg ccagctcgtt ttttggtgtc acgctgggat
     2821 tgttcgatta tctggcggat ctgtttaaga ttgataactc ccacgccggg cgtttcaaaa
     2881 ccgtgctgtt aaccttcctg ccacccgcgt tgttgtatct gatcttcccg aacggcttta
     2941 tttacgggat cggcggtgcc ggactgtgcg ccaccatctg ggcggtaata attcctgctg
     3001 tactggcaat caaagcccgc aagaagtttc ccaatcagat gttcacggtc tggggcggaa
     3061 atcttattcc ggcgattgtc attctctttg gcataaccgt aattttatgc tggttcggta
     3121 acgtctttaa cgtgttacct aaatttggct aaatccttca ggaagccagc cattcgctgg
     3181 cttcttgcct gtcaggaaat cacttatgtc caaatggcaa ctcgcctgat tctccttcac
     3241 cacgtatgct ttgcgtcacc ttactaatca ggacgcttta gcccatgtcc cgctttttga
     3301 tttgtagttt tgccctggtt ttactttatc ccgcagggat tgatatgtac ctcgttggtt
     3361 taccgcgcat cgccgccgat ctcaatgcca gcgaagcgca gttgcatatt gcgttctccg
     3421 tttatctggc ggggatggcg gctgcgatgt tatttgccgg taaagtggcc gatcgttcag
     3481 ggagaaagcc tgtcgccata cccggcgcgg cgctatttat tatcgcctcg gtgttctgtt
     3541 cactggctga aaccagcgcg ttatttcttg cagggcgatt tctacaggga ctgggcgcgg
     3601 gctgttgtta cgtagtggcg ttcgccattt tgcgcgacac gctggatgat cgacgtcggg
     3661 ctaaagtgct gtcgttactc aacggcatta cctgcatcat tccggtgtta gcgccggtgc
     3721 tcggacacct gattatgctt aaattccctt ggcagagtct gttctggacg atggcaacga
     3781 tgggcatcgc ggtactgatg ttgtctttgt ttattttaaa agaaacgcgc cctgcggccc
     3841 ccgcagcttc ggacaaacca cgagaaaata gcgagtcact gcttaaccgg tttttcctca
     3901 gccgtgttgt tatcaccacc ctcagcgttt cggtgatcct caccttcgtc aacacgtcgc
     3961 cagtattgct gatggaaatc atgggttttg agcgcggaga atacgccacc attatggcgt
     4021 tgaccgctgg cgtcagcatg accgtttcat tctccacgcc gtttgcgctg ggaattttta
     4081 agccacgtac gttgatgatc acctcacagg tgttattcct ggcggcgggg atcacccttg
     4141 ccgtttcacc ttcccatgcg gtttcgctgt ttggtatcac gctgatttgc gccggtttct
     4201 cggtaggttt tggtgtggcg atgagtcagg cgttagggcc gttttcatta cgcgcgggcg
     4261 tagccagctc gaccttaggt attgcgcaag tttgcggttc gtcactgtgg atttggctgg
     4321 cggcggtggt tggtatcggc gcatggaata tgctgatcgg gattctgatt gcctgtagca
     4381 tagtaagcct gttgctgatt atgttcgtcg cgcctggacg cccggttgcc gctcatgaag
     4441 aaatccatca ccacgcttga tctcaatctg ctgctctgtc tgcaactgct gatgcaggag
     4501 cgcagcgtga ccaaagcggc gaagcgaatg aacgtgaccc cttcggcggt gagtaagtcg
     4561 ctggcaaagt taagagcgtg gtttgacgac ccgctctttg tgaactcacc gctggggctg
     4621 tcgcccacac cgctaatggt cagcatggag caaaatctgg cggagtggat gcaaatgagc
     4681 aacctgctgc tggataaacc gcaccaccag acaccgcgcg gcctgaagtt tgagctggca
     4741 gcagaatcac cgctaatgat gatcatgctt aatgcgctgt cgaaacggat ctaccaacgt
     4801 tacccgcagg cgaccatcaa attacgtaac tgggattacg attccttaga tgccattact
     4861 cgtggtgaag tggatattgg tttttccggt cgcgaaagtc atccccgttc gcgggagctg
     4921 ttaagctcgc taccgttagc cattgattat gaagtgctgt ttagtgatgt gccctgcgtc
     4981 tggttacgca aagatcatcc ggcactgcat gaagcatgga atctggacac ctttttacgt
     5041 tatccgcata tcagcatttg ctgggaacag agcgatacct gggcgctgga caatgtgtta
     5101 caggagctgg ggcgcgaacg cacgattgct atgagcctgc cggaattcga gcagtcactg
     5161 tttatggcag cacagcccga caatctgcta ctggcgaccg cgccgcgcta ctgccagtac
     5221 tacaatcaac tccaccaact gccgttggtt gctcttcctc tcccgtttga tgaaagccag
     5281 caaaaaaagc tggaagtccc ttttaccctt ctgtggcata aacggaacag ccgtaatccg
     5341 aagatcgtct ggttacggga aaccattaaa aatctttacg cgtcgatgac ataaccgaat
     5401 cgtatgaaac gcgacccaat ttcacaatga aatgtaaaaa agttgtaata agcttatctg
     5461 aatcgaactt ttagccgctt tagtctatcc atcattccag taaatgatta ctcttgtatt
     5521 cataatggac cattaagcat ggagcgaaaa atggcgactc actttgcccg agggatttta
     5581 acggaaggac atctgatttc tgttcgtctc ccctcccagt gtcatcaaga agcccgaaac
     5641 attccccctc atcgtcaaag ccgttttctg gcgtccagag gtttactcgc agaactgatg
     5701 ttcatgctgt atggcattgg cgaattgccg gaaatcgtca ccctgccgaa aggaaaaccg
     5761 gttttcagcg ataaaaattt gccttcgttt tctatttcct atgccgggaa tatggtcggc
     5821 gtggcgttaa caaccgaagg tgaatgtggc ctcgatatgg aactacagcg tgcgacacgc
     5881 gggtttcata gcccacacgc gcccgataac cacacctttt ccagcaatga atcgctatgg
     5941 atcagtaaac aaaacgatcc taacgaagcg cgggcgcagc tcatcacgct gcgccggagc
     6001 gtgctaaaac taaccggtga tgttttgaat gacgatccgc gcgatctgca actactgccc
     6061 attgccggac gcctgaaatg tgctcatgta aatcatgtag aagcgttatg cgacgcggag
     6121 gacgtgctgg tgtggtccgt ggcggtcacg cccacgattg aaaagctcag tgtctgggag
     6181 ttagatggca aacacggctg gaaaagcctg cccgatattc acagccgcgc caacaatcct
     6241 accagccgga tgatgcgttt tgcccagctc tctaccgtga aggctttttc gccaaattga
     6301 tagacaacca caggagtcat catgtctgaa aaattgcagg tggttacgtt actggggagc
     6361 ctgcgcaaag gctcatttaa tggcatggtt gcacgtaccc tgccgaaaat tgctccggcg
     6421 agcatggaaa tcaatgcgtt accttccatt gccgacattc ccctgtatga cgctgacgta
     6481 cagcaggaag acggttttcc agcaacagtt gaagctctgg cggaacagat ccgtcaggct
     6541 gacggtgtgg tgatcgtcac gccggaatat aactactcgg taccgggtgg gctgaaaaat
     6601 gccatcgact ggctttcccg cctgccggat caaccgctgg ctggtaaacc ggtattgatt
     6661 cagaccagct caatgggcgt gattggcggc gcgcgctgtc agtatcacct gcgccagatt
     6721 ctggttttcc tcgatgcaat ggtgatgaac aagccagaat ttatgggcgg cgtgattcag
     6781 aacaaagttg atccgcaaac cggggaagtg attgatcaga gtacgctgga ccacctgacc
     6841 gggcaattga ccgcatttgg tgagtttatt cagcgagtta agatctaaat aaaaaacccg
     6901 ccagcaatca tgcatggcgg gtttttaacg cgctatcgat tttagtgagc gtcgataaag
     6961 acaatcttca ggataaacag cagcgcaacg atgattacgc acgggctaag gtcacgcaga
     7021 cgtccggtac caatcttcat cacgcagtag gagataaagc ccagcgcgat accttcagta
     7081 atcgagaagc tgaacggcat catcacagcg gtaataaacg ccggaacaga ttcagtaaga
     7141 tcctgccagt tcacgcgagc aagacttgag gtcatcaaca cgccaacgta aatcagcgca
     7201 ccagctgcag cgtagcctgg caccatcccc gccagcggcg acaggaagat aaccagcagg
     7261 aacagcagac caacaaccac tgccgtcaga ccggtacgac cgccaacgga aacgccggaa
     7321 gaagattcaa tatacgccgt aacagaagaa gtaccgataa acgaaccggt caccgaagag
     7381 atactgtcga catacagcgc ctgcttcatg cgcgggaatt tacctttctc atcagccaga
     7441 cctgctttat cggtcacgcc aatcagcgta ccggaggagt caaacaagtt gaccagcatg
     7501 aaagagaaaa tcacccctgc cagcccaagg ttaaacgacc cggctaaatc tacatgaccc
     7561 acgacactca ttacgctcgg cggcgcagaa acgatgccat tatagtggac atcacccagc
     7621 atccagccca gcagcgttgt caccacgatg gaaaccagca ccgccgcgtg aatattgcgc
     7681 gaggccagaa tggcgatgat gaagaagccg aggatcccca gcagcacgct gtgagaagtc
     7741 agattaccga tgctcaccag cgtttccggg ttagcgacaa tcacacctgc gtttttcagc
     7801 cccatcatgc caataaacag accgatacca ctggtgatgc ccacacgtag acttaccggg
     7861 atgttggcta tcatccagta gcgaacgcgg aaaatcgtca gtaacagcag accaatcgcg
     7921 ccccagaaga ttgcgcccat cccgacttgc cacggcaagc ccatcgcctg tacaacgaca
     7981 aaagcgaaga acgcattcag gcccattgcc ggtgccagtg caactggcag gttagcaaac
     8041 agccccatca taatactgcc gaatgcagca atcagacagg tcgtgacgaa gacggcgctg
     8101 gtatccatgc cagcaacgcc aagaatttgc gggttaacaa aaacgatgta aaccatcgtc
     8161 aggaaggtgg taaaaccggc gatcacttcg gtccgtgccg tcgtgccatg ttcgcgcagt
     8221 ttaaacacgc gttccagcat cccctggcca gaagcctggg ttgtgtgttg ttgactcatt
     8281 atctatttcc gaacaaggag ggaaaatccg tcgctatcgt ataccaaaat gagacaatat
     8341 ggacggctga agataacttt tttttcattg tatttgctta tacggcaacg attgcgtcat
     8401 gcgaattggc atgacgaaaa cgttaaacta acgataaagg aaagacatgt cccagataga
     8461 agcggtattt ttcgactgcg acggtacgct ggtcgatagc gaggtcattt gctctcgcgc
     8521 ctatgtcacg atgttcaggg aatttggcat tcacgttgac ctggaagaga tttttacccg
     8581 ttttaaaggc gtaaaactct acgaaatcat cgacatcatc agtaaagaac agggcgtgac
     8641 gatggtccag gccgatgccg agcacgttta ccgcgcagaa gtagcacgtc tgtttgatac
     8701 cgaacttgag gacatcgcag gagcgaacga attgctggca agcattaacg tgccgatgtg
     8761 cgtcgtctct aacggtccgg tcagcaaaat gcagcattcg cttggtaagc tcaacatgct
     8821 gcactacttc ccggaaaaac tgttcagcgg ctatgatatc cagcgttgga agcccgatcc
     8881 ggcattaatg ttccatgcgg caaaagcgat gaatgtgaac gttgaaaact gcattctggt
     8941 cgatgactct agcgccggtg cgcaatcagg gatagatgcc ggaatggaag tgttttactt
     9001 ctgcgctgac ccgcacaata aaccgatcgt tcacccgaaa gtcaccacct ttacccatct
     9061 ttcgcaatta ccggaactgt ggaaggcgcg tggttgggat attacggcat agttattcac
     9121 actcccttca cttactccac ttaaattggc acgcaaaggt aagtaaaggg agtttgatat
     9181 gtctgtttca cgtcgggtaa ttcatcacgg actgtatttt gcggttttag ggccgttaat
     9241 tggtgttctg tttcttgtcc tctacatatt cttcgcaaaa gaaccgctga ttcttttggt
     9301 aataatacag gtacttccat tgtttctctt attgtcgata actacgggag ctattcctgc
     9361 aatgttaact ggcgtaatgg tcgcctgtct gccggaaaag atcgggtcac agaaacgtta
     9421 tcgttgtctg gttggcggca taggtggagt cgttatcacc gagatctatt gcgctgttat
     9481 catacatatt aaggacatgg cttcctcagc gttatttgaa aacattcttt ctggtgaaaa
     9541 tctcgttgtc cgcatcatcc cagcattgct ggcaggcgtg gtgatgagca gaatcattac
     9601 acgtctaccc ggattggata tttcatgtcc tgaaacagac tctttaagtt aagtgggatg
     9661 ctttatctat ttgtcattaa atgttggaga acaggccgga gcgtaattca cacatccggc
     9721 cttattttta agcttaaccg agcgctaatt ctgcggcagc ggctttatca gcgatgacta
     9781 tcagcgaagg gtgcaactgc aatacggaag caggaacatc ttcggtaacc ggaccttgca
     9841 gaacattttt cagtgcctgc gctttacccg cgccactgac aattatcagc aagtttttcg
     9901 ccgccatgat gcttttcggc cccatcgtca cgtagctgtc aggcactaat gaaaagtcgc
     9961 cgcccaactc gccatgcgcc acaatatcaa ccatctctcc ctggatcggg aactccacgg
    10021 tttgctcatg gaaatgggtc gtatttggca gattgccaca aaaatgacca tctacaccta
    10081 atcccaacac caccaaatcc aggccgcctt cacgcgccag tttctgatca tgctcgcggt
    10141 agttatcaat agtgagcttc tggatattct cttctttgat ccctgcaggg gtgaaaaaca
    10201 gattacgcag attggtaatc gttacgcctt ccccttcttt accgcgaaat ggaatttcat
    10261 cgaaattgta gaaatagcag ttatcgtacc agggcttacc tttaaccaga gtgatgagat
    10321 attcgtacat gcctttgggc gtgctaccgg cggtaattgc caggttaacg cgacgcatct
    10381 ttgacatata accaagcaga tgatgtgccg cgacacggct catttcctgg taatcttcgg
    10441 taatgatcaa tttcatctta atgccttata ttatttgaat aatttctgta caaaggctgc
    10501 ataggctggt cgccagacat ccatttcatg gttcagaccg ggatattcct ggtaatcaaa
    10561 cttaattttt ttctgctcaa gctcagtttt cagcccggcg atatccttgc cggttacgac
    10621 atctttatcg cccacgacca cagtaaaatt acgtagttgc tggttaatag ctgccggctc
    10681 gttcagccgg gcagcgaccc cttcatctgg tacggtcgta gtggtaacac cactgaatgt
    10741 ggccagccag ccaaagcttt ccagatgatt cattcccgat accagtgcct gatacccacc
    10801 ttgtgaaagc cctgccagtg cgcgaccatc ggcatcttta cggacattaa aacgcttgct
    10861 aatcagcggg ataatatcgt tcatcagttc gcgatctgcc gctttagcat ttaacggata
    10921 aaagacttta cgtctctcct gaggaacgaa atcttcggga ataatgccct tcgcatcggt
    10981 ttcggtatcc gggatcacca ccagcatcgg tttaattttc ccttcagcca gcaggttatc
    11041 catgatttgc gggatacgcc cctgatcaat agcggaacgt ccggtatcac caaagccgtg
    11101 atagaaatag agcactggca aaggctcgcc tatgccgggg tatcctggcg gggtccagac
    11161 atacatctga cgttcggatt gcaatgcgtt ggagtggtaa gttatggcga tcaaatcacc
    11221 atgcacaaca gaacgtgtat ccagataact gcccggtacc agaatcatac tggagttaac
    11281 ctgacgctga ggcttagtca ttgccgtgcc tgtatcaatg ctgcgtacac catcaacatt
    11341 gaaaaaatat tcgtacaggt tgcctttcag gacaggtgtg cgccacgacc agactcccgc
    11401 ttcgtctttg gtcatcgggt gaatattgtc cggaaccgga acacccacca ctacggagac
    11461 attttttgcc ccaggggcaa agtagcgaaa ggtcacgctg ttatcggtat tgacctgagt
    11521 cacatactgt ttcaccggaa tagtgggtgc cgggctggct ggcatatccg ctgctatggc
    11581 ccattgagca gaaataccgc tggcaatagc cagcgttaaa gcagcaattt ttatattcat
    11641 cgtaatattc cttttattaa gttccattac caccagattt cagtctgggc accgacagca
    11701 aactggtcgt ctttattgtc ttcaaaggtg aagccatcca gttcattttc cagagctttg
    11761 atatacgtag cgtagaaacg aatttccggg cgcgaggtca acatactggt attgacttta
    11821 aaggtatgga agagcgtggt tttataacca gattcattat atttattgct attcgcatct
    11881 ttgttttgct gggtgaaata gcccagttca acacctgtct ggttatattg gtcccaaata
    11941 taggccgggc gaacaaccgc acgaatagat tcgaaatcag aatgtgcgcc tgtttcgtag
    12001 ctataaatat cgttaccgaa ggagtaaaca atcgcgttag ccacaatgaa atgatcgccg
    12061 atataagctt cgccctgcga agtcagacga actgcagttc cgcctgtatg atcaccataa
    12121 taacgaccat taaaggtagt aaatggactt gcaccagcat aacgaccaaa gttactggcg
    12181 atagagttat tcgcgaccag gaaggagaat tcgttgaagc cacctttatc aaatttctgc
    12241 gttaaagatg tgccaaacat ccaggtatct ttccagtcat aatacccgtt attatcctga
    12301 ttatcttttt ccgatgcgct ttcgtttgcc gtgacataac gaccactcac cattaaggtg
    12361 gccctatccc ataacgggat atctttatag cgtaaatcaa tagtattggt attaatctgc
    12421 tgcttgtttt gcaggctgcg atcgtaatca tcgacatctt cgcgaaccag cgcgatatca
    12481 attttacccg gaccgacttt ccagttttcc agacctacac ctgcggcagc atcggtacgc
    12541 tgcgttttcc agtcaagcat ctggatttca attttcggcg caccgtgttt acccacccag
    12601 aaatcagcct ctggcgcaaa gggcaggaaa cctttggtgg taacatacat atcagagaac
    12661 tgcataaagt tctcgccacc ggcgttatcg ccaaaccagc cagtagagta ctgctgaccc
    12721 acgttaccat ccatcatcac gatggcatca acccgtttgc cgttttcgtt gtagacacgt
    12781 tgttttaact gcaaatcaaa ccagccggag tattcgttac caaagcggcc caaagaacca
    12841 attgcccatg atttaggtga accatgagag gctgttcccc aaccagaacg gtagtagccg
    12901 ttgtaactaa agccgatttc atctttcaca aatttactga aatctttcag agtcatggaa
    12961 gaataaatcg agcctgtctc actggcattt ttctcttcat ttttaaccag tacagcatca
    13021 ggctttgcgg cacgactggt cgggaatgga ttggcggcat acccttgatc attcgtactt
    13081 acgctacgat tcaccgtcgc tggcgtatac tttttcttct cttcatcttt atattttttc
    13141 agttcgcttt gcgtttctct taacgccttt tctaatagct caaggcgttg ttccactgtt
    13201 aatgattcag ccaatgattg tgcggaaaag gctaacggtg ccattaataa gatggcagag
    13261 gtaataatat ttcgtctaaa cataatatcc ctttatggtg caaagaaaaa attaacgcat
    13321 cgcatccaga ctgttctgaa tgcgacgata attaaggtgc cttattgatt atttttttaa
    13381 tgatagcccc cgagttttga tcacttctgc ataccatccg aagcttttct tgcgcgttct
    13441 tgtgaggctt ccttcaccat tatcatcacg atccacataa ataaagccgt agcgcttaga
    13501 catttgtgaa tgagatgcac tgactaaatc aattggaccc caactggtgt accccataat
    13561 atccacacca tcggcaatcg cttcatttac ctgtaccagg tgatcgttta aataagtaat
    13621 tcgataatcg tcctgtatcg aaccatccgc ttcaacgctg tcttttgcgc ctaatccatt
    13681 ctcgacaata aataacggtt tttgataacg atcccaaagc gtatttaaca gaatccgtaa
    13741 tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat ttgggatcat
    13801 attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa cacaaccggt
    13861 catgtaataa ctaaaagaga tgaaatctac ggtatgtttt aaatcttctg cgtcactttc
    13921 agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc cgggatactg
    13981 accacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca tggcctgcaa
    14041 catatcctgt ggctggcagg tgagggggta aactagccca ccgagaagca tattgccaat
    14101 tttcgcttcg gggatcaggc tatgacaagc tttaactgcc cgcgcactgg caaccagttg
    14161 atgatggatc gcctgataaa cttccgcctc gcaactctct tctgccagcc ccacgcccgt
    14221 gaatggcgcg tgtaatgaca tgttgatttc attaaatgtc agccataaca caactttatg
    14281 ttggtagcgg gtgaagaccg tgcgggcgta atgctcgaag tgatcgatga ccgcccgatt
    14341 agcccaaccg ccgtagtttt tcaccagccc atatggcatt tcgtaatggg ataacgttac
    14401 cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa acgctaaccc
    14461 cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg caatggaaat
    14521 acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt aacggtgata
    14581 aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt ccatttttcc
    14641 cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt cctgccaggc
    14701 gccttccacc tgattggcag ctgttgcacc accccaaaga aatgtttctg gaaatgcttt
    14761 cataattaac tccttttatc gttagcgaat gatggataac agcggctcac cagcgtttat
    14821 ctgcgccgtg ccgtggggta atacgtccgt aaaatcatcg ctattactga ttaataccgg
    14881 cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa tcagccgatc
    14941 gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc cgtccagttt
    15001 tacggtgtcg ataccgacat gaatcaggat ctccacgcca tcatctgact caatgccaat
    15061 ggcgtgtaat gtggcgaaca acgaagcaat ttgccccgcc accggagaac gcacttcacc
    15121 aacagagggc agaatggcaa tacctttacc caacaggccg ctggcaaacg tggtatcagc
    15181 gacgtgaatg agcggcacaa tatctcccgt catcggtgaa cagataccgc cctgctcagg
    15241 tgtaataacc tctggtgttt tctcttgctg ggcaacttgc gctgactgac gtttagcggt
    15301 gatgaaatga agcattaccg taccgacaaa tgcgcaaccg atggcaatga caccaccaat
    15361 aacgctggcc catacggtga aatcaatccc cgttgacggg atggtttgca tgaaggtgaa
    15421 aatacctggc aaaccaaagg agtaaacttt cgtttgcgcg tagccaatga tggtggcccc
    15481 caatgcccca cttatgcagg cgataacgaa ggggtactta cgcggcaggt tgacgccata
    15541 tactgccggt tcggtgatac caaacagccc cgtcaacgcc gccgatcccg ctaccacttt
    15601 tttctgcgca tcgcgttcgc agaggaagac gccgagcgcc gccccgacct gcgccataat
    15661 ggcgggcatt aacagcggga tcatggtgtc gtagcccagc acggtgaagt tattgataat
    15721 taaaggtatc aaaccccaat gcagaccgaa catcacaaag atttgccaga agccgcccat
    15781 taccgcgcct gcaaatgcag gaaccgcctg ataaagccag agataaccgg cagcaatcag
    15841 ttcacttatc caggttgata gcggccccac cagcagaaat gtgatgggcg tgatgaccat
    15901 cagacatagc aagggtgtga agaaattttt gattgccgaa ggtaaccatg cattaagtcg
    15961 gcgttccaga atgctgcaca accaggcaga aaaaataatg ggaataaccg atgacgagta
    16021 attcaacaat gtgaccggaa tacccaggaa atccaggccc agcgcatcca ctttttgccc
    16081 gttctcgaag gcagtcagaa ttaatggatg cactaacgct ccgccaatca ccatggcagt
    16141 aaatggattg ccgccgaagc gtttccctgc ggtgtagccc aggattatcg ggaagaacca
    16201 aaacaaggca tcacttgcgc taaataaaat taaataagta ccactttgtt cggtcgtcca
    16261 ctgaaaagtg agtgccagag ccaacatacc tttcaggatc ccggttgccg ccatcaaacc
    16321 gatcagaggc gtaaaaatac ctgaaataac ataaacaaag cggtttaaca gattaccttt
    16381 atcatcattt tccggtgcct gttgcgcttt ttcgccaagg cctgccacgc tgttaaccgc
    16441 caggaagaca tcggccacat ggttacctat gacaacctga aactggccac cgctttccac
    16501 caccataata ataccggggg tctttttcag tacctcagct tgcgctttgc tttcatcctt
    16561 taacttaaaa cgtaatcgcg ttgcacaatg catcagactc acaatgttat ctgcgccccc
    16621 gactcctgcg actattgttc tggctaactc cgtcataact tgccctctac cgctttgcgg
    16681 caaaactcca aaaaaaaacc tgaaaaaaac ggcctgacgt gaatcaagca atttttttca
    16741 ggttttgccc gcttagtgcg gtaacaatcc tttattcagt aataatattt cagtgttctt
    16801 tgcgcacgcg ctctatattt atggctaaaa acataatctc tgcaggtgag attttacgtt
    16861 gatactgcaa accaataaaa atggcgatcc gctccgcaca ttgccatgct tgcgggtaat
    16921 tttgctttac tgcttgttgt aatgattcat cactatcgtt aatcgatgca tgttcaagaa
    16981 tacgccagga taaaaatttc agatgcgtaa ccagtcgctg ataactcaag ctttcttcct
    17041 ggtaattaag gctgaactga aattttatta attgcagcat ttctcgcatt aattgcgtga
    17101 cacctgcaac atcctccata tttccgctca tttgggcact gaccagatgc atggcaataa
    17161 agcccacttc atctttcggt aactgcacgc ccaaccgttt atcaataatg gttaacgctt
    17221 cttcccctag ctggaactct ttcgggtaaa gtcgctggat atcccacagc agcgggttgg
    17281 gcagtagcac gttttgctga aagcgtttaa tcgcaaactg gcaatggtca gttagcgaga
    17341 tataaatact gtcctgcaac tttcccagac gctcctgcgc cagagagata atacgatcac
    17401 aggttgccat cacctcaaga ggcatatgac ttaagagttc gcttaatcgc ccgttcagtt
    17461 catgactgct caaggcatac tctttttcta ttccacttga gttaattctt tcgcctgggc
    17521 gtttttgaaa gccaattccg cgccccatga cgactttttc ccgctgttga tcatcaataa
    17581 ccaccacaac attattgttg agaattttgg tgatttgcat gttcatagca aggacctttt
    17641 ttataaacaa aaaaacccga cttcaccagt attctctggt tatgtcaggt tttgcctgcg
    17701 aatgcagtaa caatccagtc attaattaat ggtttttata acgaacatcc aggttcgaaa
    17761 attaatttag ttgcgtgcag aaaaaatagc aatgctctat ttataaaagt atgaccatgc
    17821 tcgcagttat
//
LOCUS       DAZFJL010000097        17668 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFJL010000097 DAZFJL010000000
VERSION     DAZFJL010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.097"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            65..583
                     /gene="yfiR"
                     /locus_tag="ACHJ8C_003229"
     CDS             65..583
                     /gene="yfiR"
                     /locus_tag="ACHJ8C_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO5553037.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            573..1799
                     /gene="dgcN"
                     /locus_tag="ACHJ8C_003230"
     CDS             573..1799
                     /gene="dgcN"
                     /locus_tag="ACHJ8C_003230"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO5553038.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTACILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAENLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            1815..2297
                     /gene="yfiB"
                     /locus_tag="ACHJ8C_003231"
     CDS             1815..2297
                     /gene="yfiB"
                     /locus_tag="ACHJ8C_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO5553039.1"
                     /translation="MIKHLLAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     VLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITAP
                     "
     gene            complement(2374..2721)
                     /gene="rplS"
                     /locus_tag="ACHJ8C_003232"
     CDS             complement(2374..2721)
                     /gene="rplS"
                     /locus_tag="ACHJ8C_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO5553040.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2763..3530)
                     /gene="trmD"
                     /locus_tag="ACHJ8C_003233"
     CDS             complement(2763..3530)
                     /gene="trmD"
                     /locus_tag="ACHJ8C_003233"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO5553041.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHVQQQHKHDGMA"
     gene            complement(3561..4109)
                     /gene="rimM"
                     /locus_tag="ACHJ8C_003234"
     CDS             complement(3561..4109)
                     /gene="rimM"
                     /locus_tag="ACHJ8C_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO5553042.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4128..4376)
                     /gene="rpsP"
                     /locus_tag="ACHJ8C_003235"
     CDS             complement(4128..4376)
                     /gene="rpsP"
                     /locus_tag="ACHJ8C_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO5553043.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4513..5874)
                     /gene="ffh"
                     /locus_tag="ACHJ8C_003236"
     CDS             complement(4513..5874)
                     /gene="ffh"
                     /locus_tag="ACHJ8C_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO5553044.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6041..6832
                     /gene="ypjD"
                     /locus_tag="ACHJ8C_003237"
     CDS             6041..6832
                     /gene="ypjD"
                     /locus_tag="ACHJ8C_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO5553045.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6853..8139
                     /gene="yfjD"
                     /locus_tag="ACHJ8C_003238"
     CDS             6853..8139
                     /gene="yfjD"
                     /locus_tag="ACHJ8C_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO5553046.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8194..8787)
                     /gene="grpE"
                     /locus_tag="ACHJ8C_003239"
     CDS             complement(8194..8787)
                     /gene="grpE"
                     /locus_tag="ACHJ8C_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO5553047.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            8910..9788
                     /gene="nadK"
                     /locus_tag="ACHJ8C_003240"
     CDS             8910..9788
                     /gene="nadK"
                     /locus_tag="ACHJ8C_003240"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO5553048.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQQADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            9874..11535
                     /gene="recN"
                     /locus_tag="ACHJ8C_003241"
     CDS             9874..11535
                     /gene="recN"
                     /locus_tag="ACHJ8C_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO5553049.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSQLQEMT
                     ARYHLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYANELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            11684..12025
                     /gene="bamE"
                     /locus_tag="ACHJ8C_003242"
     CDS             11684..12025
                     /gene="bamE"
                     /locus_tag="ACHJ8C_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO5553050.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12087..12377)
                     /gene="yfjF"
                     /locus_tag="ACHJ8C_003243"
     CDS             complement(12087..12377)
                     /gene="yfjF"
                     /locus_tag="ACHJ8C_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO5553051.1"
                     /translation="MPGKIAVEVAYALPKKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDIVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12367..12804)
                     /gene="ratA"
                     /locus_tag="ACHJ8C_003244"
     CDS             complement(12367..12804)
                     /gene="ratA"
                     /locus_tag="ACHJ8C_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO5553052.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMSLVDGPFKKLIGGWKFTPLSQDACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            12975..13457
                     /gene="smpB"
                     /locus_tag="ACHJ8C_003245"
     CDS             12975..13457
                     /gene="smpB"
                     /locus_tag="ACHJ8C_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO5553053.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            13672..14034
                     /gene="ssrA"
                     /locus_tag="ACHJ8C_003246"
     tmRNA           13672..14034
                     /gene="ssrA"
                     /locus_tag="ACHJ8C_003246"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            14307..14555
                     /locus_tag="ACHJ8C_003247"
     CDS             14307..14555
                     /locus_tag="ACHJ8C_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO5553054.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(14663..15235)
                     /gene="cdtC"
                     /locus_tag="ACHJ8C_003248"
     CDS             complement(14663..15235)
                     /gene="cdtC"
                     /locus_tag="ACHJ8C_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015987614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytolethal distending toxin type I subunit CdtC"
                     /protein_id="HFO5553055.1"
                     /translation="MKTVIVLFVLLLTGCASEPANQRNLLTQFVGNNAPVDPEPSPVL
                     VNIRNVLTGGIIRNPVGSDFNVNNWVISEVKTNDLDLISAPGGHVQIKNPDGNECFAI
                     LNGQLAVAKQCSESDRNALFTFITSDTGAVQIKSIGSGQCLGNGESITDFRLKKCVDD
                     LGRPFDTVPPGLLWMLNPPLSPAIMSPLTS"
     gene            complement(15232..16053)
                     /gene="cdtB"
                     /locus_tag="ACHJ8C_003249"
     CDS             complement(15232..16053)
                     /gene="cdtB"
                     /locus_tag="ACHJ8C_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytolethal distending toxin type I nuclease
                     subunit CdtB"
                     /protein_id="HFO5553056.1"
                     /translation="MKKLLFLLMILPGISFADLSDFKVATWNLQGSNAPTENKWNTHV
                     RQLVTGSGAVDILMVQEAGAVPASATLTEREFSTPGIPMNEYIWNTGTNSRPQELFIY
                     FSRVDAFANRVNLAIVSNRRADEVIVLPPPTVVSRPIIGIRIGNDVFFSTHALANRGV
                     DSGAIVNSVFEFFNRQTDPIRQAANWMIAGDFNRSPATLFSTLEPGIRNHVNIIAPPD
                     PTQASGGVLDYAVVGNSVSFVLPLLRASLLFGLLRGQIASDHFPVGFIPGRGARR"
     gene            complement(16050..16763)
                     /gene="cdtA"
                     /locus_tag="ACHJ8C_003250"
     CDS             complement(16050..16763)
                     /gene="cdtA"
                     /locus_tag="ACHJ8C_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytolethal distending toxin type I subunit CdtA"
                     /protein_id="HFO5553057.1"
                     /translation="MDKKLIAFLCTLIITGCSNGIGDSPSPPGKNVELVGIPGQGIAV
                     TSNGATPTLGANNTEFPEVSIMSTGGALLTIWARPVRNWLWGYTPFDSVNFGENRNWK
                     VVDGKDAGTVKFVNVAQGTCMEAFKNGVIHNTCDDNSLSQEFQLLPSTNGNVLIRSSA
                     LQTCIRADYLSRTILSPFAFTITLEKCPGAKEETQEMLWAISPPVRAAKPNLIKPELR
                     PFRPLPIPPHDKPDGMEGV"
     gene            complement(17111..17380)
                     /locus_tag="ACHJ8C_003251"
     CDS             complement(17111..17380)
                     /locus_tag="ACHJ8C_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5553058.1"
                     /translation="MNVLKKLMQRLCGYGKHDDREHGGLLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            complement(17382..>17668)
                     /locus_tag="ACHJ8C_003252"
     CDS             complement(17382..>17668)
                     /locus_tag="ACHJ8C_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5553059.1"
                     /translation="GPVGATGPQGPKGDPGETQIRFRLGPGNIIETNSNGWFPDTDGA
                     LITGLTFLDPKDATQVQGLFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT         4584 a         4605 c         4019 g         4460 t
ORIGIN      
        1 tattttaatg agcattctga aaaaatatcg atgcatttcg agcgaagatg gtggggatcc
       61 ctgaatgcgt ttttctcacc gactgttctt acttctgatt ctcctgctga cgggagcacc
      121 aatactcgct caggagcctt ctgacgttgc gaaaaacgtg cgtatgatgg tttccggtat
      181 tgtcagttac acccgctggc ctgcgctatc agggccacca aagttatgta tcttttcatc
      241 ttcacgtttt agtaccgccc tacaagagaa tgccgcgaca tctttgcctt atctgcctgt
      301 catcattcac actcaacaag aggcgatgat ttcaggctgc aatggttttt attttggcaa
      361 tgagtcaccg acattccaga tggaattaac cgaacaatat ccgtcaaagg cgttattatt
      421 aattgccgaa caaaataccg agtgcattat tggtagcgcc ttttgtctga tcatccacaa
      481 taatgacgtc agatttgccg taaacctgga tgccttatcg cgtagcggtg taaaggtcaa
      541 cccggatgtc ttgatgctcg cacggaagaa aaatgatgga taacgataat tctcttaata
      601 agcgccccac gtttaaaaga gcattacgca acatcagtat gaccagcata tttatcacta
      661 tgatgctgat ctggttgctg ctttccgtga cctcggtgct gaccctgaaa cagtacgcgc
      721 aaaaaaacct ggcactgaca gcagcaacaa tgacttacag tctggaagca gcggtcgttt
      781 ttgccgatgg ccctgcagca actgaaacac tggcagcgct gggccagcaa gggcaatttt
      841 caactgcaga agtacgtgat aagcagcaaa atattctggc atcctggcat tacacccgta
      901 aggatccagg cgatactttc agcaatttca taagccactg gctcttccct gcccccatca
      961 ttcagccgat tcgtcacaat ggcgaaacca ttggcgaagt acgcttaacc gctcgcgaca
     1021 gttcaatcag ccatttcatc tggttttcgc tcgctgtcct gaccgcctgc attctgctag
     1081 cgtcaggcat tgcaattacc ctcacccgcc atttgcacaa tggcctggtg gaagcactga
     1141 aaaatatcac cgatgtcgtg catgatgtgc gttctaaccg caatttttcc cgacgagttt
     1201 cggaagaacg tatcgctgag tttcaccgct tcgctctcga cttcaacagt ctgctggatg
     1261 aaatggaaga gtggcagctt cgtttacagg ccaaaaatgc gcagcttcta cgtaccgcgc
     1321 tacatgaccc attaaccggg ctggctaacc gcgcagcgtt tcgtagcggc atcaacacgt
     1381 tgatgaacaa ttccgatgcc cgaaaaacgt cggcgttact atttcttgat ggcgataatt
     1441 tcaaatatat caatgatacc tggggtcatg cgacgggcga tagagtcttg attgaaatag
     1501 caaaacggtt agctgaattt ggcgggctgc gacataaagc ataccgcctg ggcggcgatg
     1561 aattcgctat ggtgctctat gatgtacagt cggaatctga agtgcagcag atatgctcag
     1621 cactgacaca aatttttaat ctcccgtttg atcttcataa tggccatcag actaccatga
     1681 cattaagcat tggttacgcg atgactattg agcacgcctc tgcggaaaat ttacaagagc
     1741 ttgccgatca caatatgtat caggccaaac accagcgtgc cgaaaagctg gtgagataac
     1801 aaggatatat cgatatgata aagcacctgc tagcacccct gattttcacc tcgctaatac
     1861 ttactggctg ccagtcccct cagggaaagt ttactcctga gcaagtcgcc gctatgcaat
     1921 cttatggatt tactgaatcc gccggcgact ggtcgctggg cttatcagat gccattctgt
     1981 tcgcaaaaaa tgactacaaa ttgctcccgg aaagccagca acaaatccaa accatggcag
     2041 ccaaattggc ctcgacaggg ctaacacatg cccgtatgga tggacacacc gacaactatg
     2101 gtgaagacag ttacaacgaa gtcttatcat taaaacgggc gaatgtcgtg gccgatgcat
     2161 gggctatggg tggacaaatt ccgcgcagca atctcaccac acagggttta ggaaaaaaat
     2221 atcccatagc cagtaacaag accgcccagg gccgcgccga gaaccgccgc gtcgcagtgg
     2281 tgattactgc cccttaaaac acgagcaaac agacaaaaaa agggccagcc aattggccag
     2341 cccttcttaa caggatgtcg cttaagcgaa atcttagtta agacgctctt tgatacgagc
     2401 agccttacca gtacgctcac gcaggtagta cagtttagct ttacgaacag caccacgacg
     2461 tttgacagaa atgctgtcaa ctaccggaga gtgagtctgg aagacacgct caacgccttc
     2521 gccgttggaa attttacgaa cagtgaatgc agagtgcaga ccgcggttac gaatagcgat
     2581 aaccacgccc tcgaatgcct gcagacgttt tttggaacct tcaacaaccc atactttcac
     2641 ttccacggta tcacccggac ggaaggaagg tacgtcctgc ttcatctgct cttgttcaag
     2701 ttgcttaata atgttgctca taatttaatc tcttatcctg ggtaaactga tatctcgggg
     2761 gcttacgcca tcccatcatg tttatgttgc tgttgtacgt gttccgtttt gaactccgcc
     2821 agcaaccttg cttgctcttc agtcagagcc aggttttcca gaagttcagg tcttctaagc
     2881 caggtacggc ccagcgactg tttcaaacgc cagcgacgta tctcggcatg gttgcccgac
     2941 agtaacactg gcggaacttc catcccttct aacacctcag gccgcgtata gtgcgggcaa
     3001 tccagcaatc cttcagcaaa ggaatcttcc gttgccgagg cttcatgtcc cagtactccc
     3061 ggaataaacc gggaaacgga gtcaatcagc gtcattgctg gtaactcacc accactgaga
     3121 acgtaatcgc cgattgacca ttcttcgtca atttcggttt ggatcacgcg ctcatctata
     3181 ccttcgtagc gaccacacac cagaatcaat ttttgattcg ttgccagttc gctgacgccc
     3241 gcttgatcaa gcttgcgtcc ctgtggtgac agataaatca cctttgcgcc ttcacccgcc
     3301 gcggcttttg ctgcatgaat ggcgtcccgc aagggttgca ccatcattaa catccccggt
     3361 ccgccgccgt aaggacgatc gtccacggta cggtgccggt catgcgtgaa gtcgcgagga
     3421 ctccagctct ggatgctcag caggccattt ttaactgccc ggccagttac cccgtaatcg
     3481 gtaattgcgc ggaacatttc aggaaacagg ctaattatgc caatccacat agcgccgtct
     3541 tttaccgttt atccggtggt ttaaaaacca ggatcccaat ctacttcgat tgaacgagta
     3601 gtgagatcga ctttcttgat aacctgccca tcgaggaacg gtacgagacg ttccttgata
     3661 ccaaacgcat ctttcaggtt tgccttaatg acgagaacgt cattagatcc ggtttccatc
     3721 atatctacga ctttaccgag atcgtagcct tcagtggtta ctacctggca gcccatcagg
     3781 tctttccagt agtagtcccc ctcttcaagc tgaggcaact gcgatgaatc cacgacaatt
     3841 tcacaattcg tcagcaggtt cgccgcatca cgatcgtcaa cgcctttcag cttgatgatc
     3901 atgtcctgat tgtggtgctt ccagctttcc agctggactt gctgccactg acccgccttc
     3961 tggataaacc agggctgata gtcaaaaatg ctttcggcgt cttcggtgga agaaaacact
     4021 ctgagccacc cacgaatacc gtaagacgaa cccatttttc ccaaaacgat gggatcaaca
     4081 ggtgcttgcg cggtgagttg tttgctcatc atgaccaccg tgacagatta agctgctttg
     4141 tttacttctt tgatcagcgc agcaacgcga tcagaaatag ttgcgccctg gccaacccag
     4201 tgagcgatgc gatccagatc caggcgagtg ccttcttctt tttcgctagc gattgggttg
     4261 aagaaaccaa cgcgctcgat gaagcgaccg ttgcgtgcat tacggctgtc agcgacaaca
     4321 acctggtaga acggacgctt tttagcgccg tgacgtgcta aacgaatagt taccataaca
     4381 tcctcttgtg tgaataaaac aaccggaccc catcgaggaa cggagtccgg tgtcatatta
     4441 aaagcccgaa aattttactg atttctggag aaaatgcaat cagcagttaa taactctgtc
     4501 gcataaggtc atttagcgac caggaaatcc cggcggcatc atccctttca tgcttctcat
     4561 catcttcgcc atcccgccct tcttcatttt cttcatcatg cgctgcatgt cgtcgaactg
     4621 tttcagaaga cggttaacgt cctgcacctg cataccgcaa ccggcagcga tacgacgctt
     4681 acgcgaacct ttgatgattt ctggcttagc gcgctctttc atcgtcatcg agttgatgat
     4741 ggcttccata cgcaccagca ctttatcgtc catctgcgac ttgacgttat ccgggatctg
     4801 ccccatgccc ggcaacttgc ccatcaggct ggccatgccg cccatatttt tcatctggcg
     4861 cagctgctca agaaagtcgt tgagatcgaa gccgtcgcct tttttcagct tgctggctaa
     4921 tttctctgcc tgcgcgcggt caaccttgct ttcgatatct tcgatcagtg acagtacgtc
     4981 gcccatgccg agaatacggg aggcaatacg gtccggatgg aatggctcca gcgcctcggt
     5041 tttctcgcca acaccgagga acttgatcgg tttgccagtg atgtgacgaa tagagagcgc
     5101 cgcaccgccg cgggcatcgc cgtccacttt ggtcaacact acaccggtaa gcggtaacgc
     5161 ttcattgaat gcttttgccg tattggccgc atcctgaccg gtcatggcat cgaccacaaa
     5221 cagggtttca accggattaa tcgacgcatg gacttgtttg atctcgtcca tcatcgcttc
     5281 gtcaacgtgc agacgaccgg cagtatccac cagcagcacg tcgtagaatt tcagtttggc
     5341 ttctttcagc gccgcgttaa cgatatctac cggcttctga ccaacatcag aagggaagaa
     5401 atcaacgccc acctgctctg ccagcgtctc aagctgtttg attgccgccg ggcgatagac
     5461 gtcggcagaa accaccagca ctttcttctt gtgcttctcg cgcaggaact taccgagttt
     5521 accaacgctg gttgttttac cggcaccttg caggcccgcc atcagcacga ccgcaggcgg
     5581 ttgcgcagcc aggttcaggg tctggttctc ttcgcccatc gccgcaacca gttcgttacg
     5641 gacaattttg acgaactcct gccccggcgt caggctctta ttaacttcat gaccaaccgc
     5701 tttctctttt acgcgattga taaactcacg cactaccggc agagcgacgt ccgcctccag
     5761 cagcgccatg cgcacttcgc gcagcgtatc ttttacgttg tcttcagtga ggcgtccacg
     5821 gccactgata ttgcgcagcg tgcgcgacaa acgatcggtt aaattatcaa acattgtctc
     5881 tcgcctgggg tggaaacggt tggccgcaat cgcgacacat catcagtatt ttgccgcagt
     5941 ataacatgaa ggcgtctttg ttgttatgca acggttggag cagcgttcac ctgacgctat
     6001 actgcttctc tttcttattg ctcaaactgt cgacattact atgcccgttt ttgctctgct
     6061 cgcgcttgtc gcctactccg tcagtcttgc gctgattgtt cccggtctgc tgcaaaaaaa
     6121 cggcggctgg cggcgcatgg ctattatttc tgcggtcatc gcgctggtct gccacgcaat
     6181 cgctctggaa gcccgcatcc tgcccgacgg tgatagcgga caaaacctca gcctgctgaa
     6241 cgttggttca ttggtcagtt tgatgatctg tacggtaatg accattgtgg cttctcgcaa
     6301 tcgtggctgg ttgctgctac ccattgtcta tgcctttgcg cttatcaacc tggcgctggc
     6361 gaccttcatg cccaatgaat acatcaccca tctggaagca acgcctggga tgctggtgca
     6421 cattggctta tcgctctttt cctatgccac gctaattatc gccgccctgt acgcgctgca
     6481 actggcgtgg attgattacc aactgaagaa caagaaactg gcgtttaacc aggaaatgcc
     6541 gccattgatg agtatcgagc gtaaaatgtt ccacatcacg cagattggcg tggtgctgct
     6601 aacgctcact ctttgcactg gcctgttcta catgcacaac ctatttagca tggaaaatat
     6661 cgacaaggct gtgctctcta tcgtggcgtg gtttgtctat attgtgctgc tgtggggaca
     6721 ttatcatgaa ggatggcgtg gacgtcgcgt cgtctggttt aacgttgcgg gtgcagtcat
     6781 tctgacactg gcctacttcg gcagccgaat tgtccagcag ttaatcagct aaacccagaa
     6841 aaggagtttc ccctggaaca catttctact actacgttga tcattattct gatcatcatg
     6901 gtggtcattt cagcctattt ttccgggtcc gaaaccggaa tgatgaccct caaccgctat
     6961 cgtctgcgac atatggcgaa acagggtaat cgctcggcca agcgcgtcga aaaattgctg
     7021 cgtaagccag accgcctgat aagcctggta ttaatcggca ataacctggt caatattctt
     7081 gcctccgcgc tcggcactat tgttgggatg cgtttgtacg gcgatgcggg cgtggcaatt
     7141 gcgactggtg tgctgacttt tgtcgtactg gtttttgctg aggtattgcc gaaaaccatt
     7201 gccgcgctgt acccggaaaa agtcgcttat ccgagtagtt ttctgctggc tccgctgcaa
     7261 attttgatga tgccgctggt ctggttgctg aatgctatca cccgtatgct gatgcgcatg
     7321 atgggtatca aaaccgatat cgtggttagc ggctctttaa gcaaagaaga gttgcgtacc
     7381 atcgtgcacg aatcacgctc acaaatttcc cgtcgcaatc aggatatgct gctgtcggtg
     7441 ctcgatctgg aaaagatgac cgtcgatgac atcatggtgc cgcgcagtga aattatcggt
     7501 attgatatca acgatgactg gaaatcgatt ctgcgccaac tctcccactc acctcacggg
     7561 cgcatcgtgc tctaccgtga ttcgctggac gacgccatca gtatgctgcg tgtgcgtgaa
     7621 gcctggcgac tcatgtcgga gaaaaaagag ttcaccaaag aaaccatgct acgcgccgcg
     7681 gacgagatct attttgtccc ggagggtacg ccgctcagca cacagttggt gaagtttcag
     7741 cgcaacaaaa agaaagtcgg cctggtcgtc aacgagtatg gagacattca gggactggtg
     7801 acggtcgaag atattctcga agagattgtc ggcgatttca ccacgtcgat gtcgccaaca
     7861 cttgccgaag aggtcacgcc gcaaaacgac ggttcggtga ttattgatgg cactgccaac
     7921 gtgcgagaaa ttaacaaagc ctttaactgg catctaccgg aagatgatgc tcgcacggtt
     7981 aatggcgtca ttcttgaggc actggaagag atccctgtcg caggcacccg cgtgcgtatt
     8041 ggcgagtacg atatcgatat tctcgacgta caggacaata tgattaagca ggtaaaagtt
     8101 tttcctgtaa aaccgctgcg cgagagtgtg gcggagtaac gaaaacggcc ctgcattcgc
     8161 atgcagggcc gtgaattatt acgaaaacag aaattacgct tttgctttcg ctacagttac
     8221 catcgccgca cgaatcgtac gaccattcag cgtataaccc ttctgcataa tgcccagtac
     8281 gttacctggc gcaacgtcat cagattccac cattgcgatg gcctgatgca cattcgggtc
     8341 cagcgggacg ttagtttcgg cgatcacttc aacgccaaac ttacgcacaa catccagcat
     8401 cgacttcagc gtcagctcaa taccttcaac catcgcggac atatccgggt tagctttatc
     8461 agccacttcc agcgcacgat ccaggctatc aatcaccggc agcaattcgt tgatgaattt
     8521 ctccagcgcg aatttgtggg ctttttcgat atccagttca gtacgacgac gcaggttttc
     8581 catttcggct tttacgcgca aaatgccgtc acgttcacgg gtctgggctt cagccagctg
     8641 agcttcgaga ttcgcaattt tttcatcgcg cggatccacc tgctcagcag aagcttctgg
     8701 ctcaactgcc tcaatctctt cgtgctgatc catgataatt tcttccgggg cttgcccctc
     8761 aggcgttttc tgttctttac tactcatgaa tttctccgcg tttttttcgc attcatctcg
     8821 ctaacttcgc ttattatggg gatcagtttc agggtttcaa gggaagcact cacattgtca
     8881 tcaatcttcg caacaaggac ctcggaaaaa tgaataatca tttcaagtgt attggcattg
     8941 tgggacaccc acggcacccc actgcactga caacacatga aatgctctac cgctggctgt
     9001 gcacaaaagg ttacgaggtc atcgttgagc aacaaatcgc tcacgaactg caactgaaga
     9061 atgtgaaaac tggcacgctc gcggagattg ggcaacaggc tgatctcgcg gttgtcgttg
     9121 gcggcgacgg taatatgctg ggcgcggcgc gcacgctcgc ccgctacgat attaaagtta
     9181 ttggaatcaa ccgtggcaac ctgggtttcc tgactgacct tgatcccgat aacgcccagc
     9241 aacaattagc cgatgtgctg gaaggccact acatcagcga gaaacgtttt ttgctggaag
     9301 cgcaagtctg tcagcaagat tgccagaaac gcatcagcac ggcgattaac gaagtggtac
     9361 ttcatcctgg caaagtggcg catatgattg agttcgaagt gtatatcgac gagatctttg
     9421 cgttttcgca gcgttccgat ggactgatta tttcgacgcc aacaggctcc accgcatatt
     9481 ccctctctgc gggcggtcct attctgacac cctctctgga tgcgattacc ctggtgccca
     9541 tgttcccgca tacgttgtca gcacgaccac tggtcataaa cagcagcagc acgatccgtc
     9601 tacgtttttc gcatcgccgt aacgacctgg aaatcagttg cgacagccag atagcacttc
     9661 cgattcagga aggtgaagat gtcctgattc gtcgctgtga ttaccatctg aatctgattc
     9721 atccgaaaga ttacagttat ttcaatacat taagcaccaa gctcggctgg tcaaaaaaat
     9781 tattctaatt ttacgccagc ctctttactg tatataaaac cagtttatac tgtacacaat
     9841 aacagtaatg gtttttcata caggaaaaca actatgttgg cacaactgac catcagcaac
     9901 tttgctatcg ttcgtgagct tgagattgat tttcatagcg gcatgaccgt aataactggc
     9961 gagaccggcg cgggtaaatc tattgcaata gatgccctcg gcctttgtct cggtggtcgc
    10021 gctgaagccg acatggtgcg taccggcgct gctcgtgccg acctgtgcgc ccgtttttct
    10081 ctgaaagata cgccagcggc cctgcgttgg ctggaagaaa accagcttga agacgggcat
    10141 gaatgtttgc ttcgtcgcgt aatcagcagc gatggtcgct cccgtggttt catcaacggt
    10201 acagctgttc ctctgtcaca actgcgcgaa ctgggtcagt tgctgattca gatccatggt
    10261 cagcacgctc atcagttact caccaaaccc gagcaccaaa aattcctgct tgatggctat
    10321 gccaatgaaa cctctcagct ccaggaaatg accgcacgtt atcatttgtg gcatcaaagc
    10381 tgccgtgacc tcgcgcatca tcaacagtta agtcaggaac gcgccgctcg tgcggagctg
    10441 ctgcaatacc aattaaaaga acttaacgaa tttaatccgc agcctggaga gtttgagcaa
    10501 atcgacgaag agtacaaacg tctggcgaac agcggtcaat tgctgaccac cagccagaat
    10561 gcattggcat taatggctga cggtgaagac gcaaacctgc aaagtcaact ttacacggct
    10621 aaacaactgg tgagcgaatt gattggcatg gacagcaaac tgtccggcgt acttgatatg
    10681 ctggaagagg ctaccatcca gattgctgaa gccagtgatg aactgcgcca ctactgcgat
    10741 cgtctggatc tcgatcccaa ccgactgttt gaacttgaac agcgcatctc aaaacagatt
    10801 tcgctggcac gtaaacatca cgtcagccct gaggcattgc cacagtatta ccagtcgcta
    10861 ctggaagaac agcagcaact ggacgatcag gccgactcac aagaaacgct cgcgctggcg
    10921 gtaacgaaac atcatcagca ggcactggaa acggcgcgcg cgttacacca gcaacgccag
    10981 cattatgcaa atgagttggc acagctgatc accgatagta tgcatgcact ctctatgcca
    11041 catgggcagt ttacgatcga tgttaaattt gacgagcatc acctgggcgc tgacggcgct
    11101 gatcgtattg agtttcgggt aaccaccaac ccaggtcagc caatgcagcc tattgccaaa
    11161 gtcgcatccg gtggtgagtt gtcccgcatc gcattggcaa tccaggtcat taccgcacgt
    11221 aaaatggaaa ccccggcact gatttttgat gaagtggatg tagggattag cggcccaaca
    11281 gcggcagttg tcggcaaact gctacgtcaa cttggcgaat caactcaggt gatgtgtgtt
    11341 acccacctgc cacaagtcgc gggatgtggt catcaacact attttgtcag caaagaaacc
    11401 gatggtgcga tgacagaaac gcatatgcaa tccctggata aaaaagcgcg gttacaagag
    11461 ttggcgcgcc tgcttggtgg cagtgaagtc acacgtaaca cactggcgaa tgcgaaagaa
    11521 ctgcttgcgg cgtaaacttt tttcctgctt cacggtcaga gtaaacagca aaacgccgta
    11581 agaccggaaa gcaaaaggtt ttaaagtgat gaaaggtcta ttatcatcgg catattacag
    11641 atgagccacg tactgctcgg gcccgaaaag gaatcaaatc actatgcgct gtaaaacgct
    11701 gactgctgca gcagcagtac tattgatgtt gaccgcaggc tgttccactc tggagcgagt
    11761 ggtttaccgt cctgacatca accaggggaa ctatctgacc gctaacgacg tatccaaaat
    11821 acgtgttggc atgacgcaac aacaagttgc gtacgcattg ggtacaccgc tgatgtccga
    11881 tccatttggt acaaatacct ggttctatgt cttccgccag caacctggtc atgaaggtgt
    11941 aacgcagcaa acgctgacgc tgacctttaa cagtagcggt gtgttgacca acattgataa
    12001 caaacctgcg ctgagtggta actaataata aagttgctct caaagacgtt aaaaaaggtg
    12061 ctcaatgagc accttttttc tgtctgttat ttattcgctg atttttctgc tcgttgcctg
    12121 cgaagctctt tcggatcggc aatgagagga cgataaatct ccacccgatc gccatcatgc
    12181 acaatatcgc tgagttttgc cggacggctg taaatgccga ctttgttttt agttaaatcg
    12241 atatcggtac gcaattccag caaaccactg gcgcgaatag cttcttcaac cgtcgcgccc
    12301 tcctgcaacg tcacgcgctg caagtactgc ttcttaggta gcgcataagc cacctcaacg
    12361 gcaattttac ctggcactgt agacctcttt tgcacgaaca gtaaaagcct gcaccatatt
    12421 agctgccagc tctttaaaca cacgaccaaa ggcgagttca atcaacttat tggtaaactc
    12481 aaagtcgaga tgaaactcaa tacggcacgc gtcctggctc aacggcgtaa acttccaacc
    12541 gccgatcaat ttcttaaacg gtccatcaac cagactcatg agaatacttt gattactggt
    12601 caactggttg cgggttgtaa acgttttgct gatcccagcc ttagacacat ctaccgcagc
    12661 ggtcatctgc ccaggagtgg actccagaat ccggcttcca gtgcaccctg gtaaaaactg
    12721 aggataagac tgaacgtcat tcactaactg atacatttgc tccgcgctgt agggtaccag
    12781 tgcggtccgg ctaatctgag gcataacaat ttccatcaac aaaaatccaa caaataatat
    12841 catttatcct gctgtaaaaa aaacgctatc ccggcgctgg gtaacatcgg gatcatgcta
    12901 agatagagcc ttgtcccccg caggattgat atggggtgtt ttcgatttca gattaccgat
    12961 gattcacgac gcttatgacg aagaaaaaag cacataaacc tggttcagcg accatcgcgc
    13021 ttaacaagcg cgcccgtcac gaatacttta tcgaagaaga gttcgaagcg ggacttgccc
    13081 tgcaaggctg ggaggttaaa tccctgcgcg caggaaaagc caatatcagc gacagctatg
    13141 tccttctgcg cgacggagag gcatttctgt ttggcgctaa catcacgcca atggctgtgg
    13201 cctccacgca tgtggtgtgc gatcctaccc gtacccgcaa gttacttctc aaccagcgcg
    13261 aactggactc attgtacggt cgcgtcaatc gagaaggcta taccgtagtg gcgctctccc
    13321 tgtactggaa aaatgcctgg tgcaaagtga aaatcggcgt cgccaaaggt aagaaacagc
    13381 acgataaacg ttcagatatc aaagaacgcg aatggcaggt ggataaagcg cgtatcatga
    13441 aaaacgccca ccgttaaacc tatataagcg atactaatca aatcctcacc gcgcctccct
    13501 atccggtggc gcgaatgaac atcttgtcga ctgtcaaatc tgacacagat gtagccatcc
    13561 cattgcttaa tcgaaaaaaa atcatactat gcaggcgcta attccaggat gataacgcct
    13621 tttgggctag tcatgctgct cataaatctg gtatacttac cgttacacat tggggctgat
    13681 tctggattcg acgggatttg cgaaacccaa ggtgcatgcc gaggggcggt tggcctcgta
    13741 aaaagccgca aaaaatagtc gcaaacgacg aaaactacgc tttagcagct taataacctg
    13801 cttagagccc tctctcccta gcctccgctc ttaggacggg gatcaagaga ggtcaaaccc
    13861 aaaagagatc gcgtggaagc cctgcctggg gttgaagcgt taaaacttaa tcaggctagt
    13921 ttgttagtgg cgtgtccgtc cgcagctggc aagcgaatgt aaagactgac taagcatgta
    13981 gtaccgagga tgtaggaatt tcggacgcgg gttcaactcc cgccagctcc accactttaa
    14041 gaaggactac aaccggacag tagcaataaa tacagccact tacggacaca agccagacag
    14101 tagtcggact gaaaaaagac aaaaatatac acgtgaaatg cacgtgcact tctaaaaaac
    14161 cccagttatc accgactggg gtttttcaat ttgtaactaa gggtaacaaa aacacctccg
    14221 ttttttttca tctcactcac cttgacactg tttatttgta cagttaaaaa taatactgta
    14281 tacaaacaca gtatagaggg acttttatgc gtattgagat ctgcatagcc aaagagaaaa
    14341 tgactaaaat gccaaacggt gctgtggatg cgttaaagga agaattaact cggcgcatca
    14401 gtaagcgtta tgacgatgta gaagtgatcg taaaagccac cagcaatgat gggctttctg
    14461 ttacacgcac agcagataag gattctgcaa aaacatttgt tcaggagact ctgaaagata
    14521 cctgggagtc tgctgacgag tggtttgttc actaataaac atgtaaaatc ggtaacggct
    14581 ggaaatcatt caatactcgc actatcgaaa agcttaccag ccaaccgcgg caagttgcat
    14641 acaacgtgcc gcgttttcca gatcagctcg ttaatggaga cattattgcc ggagataatg
    14701 gtggattcag catccagagt aaccccggcg gcaccgtatc aaaaggacgc ccaagatcat
    14761 caacacattt ttttaatctg aaatctgtaa tgctctctcc attccctagg cattgaccgc
    14821 ttcctattga cttgatttgc acagccccag tatcactggt tataaatgta aacaatgcgt
    14881 tacggtcgct ttcagagcac tgcttagcca ctgccaattg cccgtttaga atagcaaagc
    14941 attcattgcc atcaggattt ttaatctgaa catgccctcc cggtgccgat atcaaatcca
    15001 aatcattagt ctttacttca gatataaccc aattatttac attaaagtca ctaccaacag
    15061 gatttcggat tatcccccct gtaagaacgt ttctgatatt aaccaatact ggactgggtt
    15121 caggatctac aggggcatta ttgccgacaa actgagtaag aagattacgc tgatttgcag
    15181 gttcagaagc acaacctgtc agcagtaaaa caaaaagcac aataactgtt ttcatcttct
    15241 cgctcctctt ccaggaataa agccaactgg aaagtgatca gaggcaattt gccctcttaa
    15301 taatccgaat aacaacgagg ccctcaacag aggaagtaca aagctcactg aatttccaac
    15361 taccgcataa tcaagaacac caccactggc ttgcgttgga tctggtggag caataatatt
    15421 tacatgatta cgaatccccg gttcaagagt tgaaaatagt gtagccggtg aacggttaaa
    15481 atcgcctgca atcatccagt tagcggcctg tcttatagga tccgtttgtc tgttgaagaa
    15541 ctcaaaaaca ctatttacaa ttgctcctga atccacgcct cgattcgcca atgcatgggt
    15601 tgagaagaaa acatcattac caattctaat gccgatgatc ggtcgtgata caacagttgg
    15661 aggtggtaat acaatcacct catcagctct tctgtttgaa acaatagcaa gatttactct
    15721 gttagcgaat gcatcaacac gtgagaaata tataaacaac tcctgtggac gactattggt
    15781 tccggtattc cagatatact cattcatcgg aataccggga gtgctaaatt ctcgctcagt
    15841 caacgttgca gaagctggta ctgcccctgc ctcctgaacc atcaggatat caacagcacc
    15901 acttcccgta acaagttgtc ggacatgtgt gttccattta ttttctgtcg gtgcatttga
    15961 accctgcaaa ttccaggttg caactttaaa atcgcttaaa tctgcaaaag aaatacccgg
    16021 caaaatcatt aacaggaata ataatttttt catactccct ccattccatc aggtttgtca
    16081 tgaggtggaa ttggcaatgg tctgaatggt cttaactctg gcttaatcag atttggtttt
    16141 gccgctctga ctggtggact tattgcccat agcatttctt gcgtttcttc ttttgcacca
    16201 gggcatttct caagggtgat tgtaaaagca aacggtgaca gtatagttct gcttaaatag
    16261 tctgctctta tacacgtctg caaggcacta cttcttataa gcacattacc attagtagaa
    16321 ggtagtaact gaaactcctg agataacgag ttatcatcac aggtattatg tatcaccccg
    16381 tttttaaagg cctccatgca agtcccctgg gcaacattaa caaatttcac tgtgccggca
    16441 tctttaccat ccacaacctt ccagttccga ttctcaccaa aatttactga atcaaaagga
    16501 gtataccccc aaagccagtt acgaacaggt ctggcccaaa tagtaagcag cgccccacca
    16561 gtgctcatta ttgaaacttc aggaaactca gtgttgttgg ctccaagtgt tggagttgca
    16621 ccgtttgaag tcacagcaat accttgtcca gggattccaa ccaattctac attttttccc
    16681 ggaggggacg gtgaatcccc gatcccattc gagcaaccag taattataag tgtgcacaaa
    16741 aatgcaatta gttttttatc cactgtattt cctcctctgc accacctctt ctttataaaa
    16801 aaatagaaac aacaaagcaa cgagaatatt actaacaaga ttaatgagaa aatgtgaaac
    16861 cagaacaaaa aacaagttta ttataacacc tgttattatt catcaggttg cgaacagcat
    16921 actcaaatat aattctttat attccccaaa aactatgcaa tatgaccata acatctacgg
    16981 ttcagtagat actcacctgt ctggaaataa gggggcaaga acactccaga cttaaatagc
    17041 cataacaaat atccccctga catggcaaca caaaaccgga accggactcc ggttttgtga
    17101 agctgtcggg ttacttcatc ccgccaatat tttcccacgt cccgtcagca cgcagaattt
    17161 gcagcggtct taccacacac tgtatctgct ttttatccgc atccagtatc accacctgcg
    17221 tgattaccct gtcctgctcc gggataatgc cattctcatc tgactccaga atgtctgccg
    17281 gtcccagtcg cagctgtgct gtaagtaacc ccccgtgttc acggtcatca tgctttccgt
    17341 acccgcacag acgctgcata agttttttta gtacgttcat gtcattctcc tgttctgcct
    17401 gtatcactgc ccacttcatc aagcccctta acatcctgcc acggcccgtc accaaacctg
    17461 acctgcaaat gctgaaacag cccctgaacc tgtgtggcat ctttggggtc aagaaaggtc
    17521 agtccggtga tgagtgcgcc atctgtatcc gggaaccagc cattgctgtt tgtctcaata
    17581 atgtttcccg gccccagacg aaaacggatt tgtgtctccc ccgggtcgcc ctttggtccc
    17641 tgaggtccgg ttgcccccac cgggccag
//
LOCUS       DAZFJL010000098        17449 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZFJL010000098 DAZFJL010000000
VERSION     DAZFJL010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.098"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..526)
                     /gene="gloB"
                     /locus_tag="ACHJ8C_003253"
     CDS             complement(<1..526)
                     /gene="gloB"
                     /locus_tag="ACHJ8C_003253"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO5553060.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAISANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLRKLSALPDDTL
                     VCCAHEYTLSNMKFA"
     gene            560..1282
                     /gene="yafS"
                     /locus_tag="ACHJ8C_003254"
     CDS             560..1282
                     /gene="yafS"
                     /locus_tag="ACHJ8C_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5553061.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(1279..1746)
                     /gene="rnhA"
                     /locus_tag="ACHJ8C_003255"
     CDS             complement(1279..1746)
                     /gene="rnhA"
                     /locus_tag="ACHJ8C_003255"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO5553062.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGHEKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            1811..2542
                     /gene="dnaQ"
                     /locus_tag="ACHJ8C_003256"
     CDS             1811..2542
                     /gene="dnaQ"
                     /locus_tag="ACHJ8C_003256"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO5553063.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNATF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            2675..2751
                     /locus_tag="ACHJ8C_003257"
     tRNA            2675..2751
                     /locus_tag="ACHJ8C_003257"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2709..2711,aa:Asp,seq:gtc)
     gene            complement(2850..3620)
                     /gene="yafV"
                     /locus_tag="ACHJ8C_003258"
     CDS             complement(2850..3620)
                     /gene="yafV"
                     /locus_tag="ACHJ8C_003258"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO5553064.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESDSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNERVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADTHQATRIDAELSMMALREYREKFPAWQDADEFTFR"
     gene            3801..4247
                     /gene="ivy"
                     /locus_tag="ACHJ8C_003259"
     CDS             3801..4247
                     /gene="ivy"
                     /locus_tag="ACHJ8C_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO5553065.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     IIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(4290..6734)
                     /gene="fadE"
                     /locus_tag="ACHJ8C_003260"
     CDS             complement(4290..6734)
                     /gene="fadE"
                     /locus_tag="ACHJ8C_003260"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO5553066.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAVLGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRIANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSKHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEDLA
                     TKPVKLPEKVRKVEAA"
     gene            6974..7552
                     /gene="lpcA"
                     /locus_tag="ACHJ8C_003261"
     CDS             6974..7552
                     /gene="lpcA"
                     /locus_tag="ACHJ8C_003261"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO5553067.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            7668..8435
                     /gene="yafJ"
                     /locus_tag="ACHJ8C_003262"
     CDS             7668..8435
                     /gene="yafJ"
                     /locus_tag="ACHJ8C_003262"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO5553068.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(8406..9146)
                     /gene="dpaA"
                     /locus_tag="ACHJ8C_003263"
     CDS             complement(8406..9146)
                     /gene="dpaA"
                     /locus_tag="ACHJ8C_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO5553069.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            9458..10207
                     /gene="yafL"
                     /locus_tag="ACHJ8C_003264"
     CDS             9458..10207
                     /gene="yafL"
                     /locus_tag="ACHJ8C_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5553070.1"
                     /translation="MSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAKSRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LVENNHFLSSDHLHNITEVAIHRLEQQLGKPYIWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            complement(10264..10620)
                     /locus_tag="ACHJ8C_003265"
     CDS             complement(10264..10620)
                     /locus_tag="ACHJ8C_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO5553071.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVSTSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIIDVLEREFSAQM"
     gene            complement(10613..10891)
                     /locus_tag="ACHJ8C_003266"
     CDS             complement(10613..10891)
                     /locus_tag="ACHJ8C_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO5553072.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            complement(10996..13089)
                     /locus_tag="ACHJ8C_003267"
     CDS             complement(10996..13089)
                     /locus_tag="ACHJ8C_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO5553073.1"
                     /translation="MAKTTKSFLALLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGAGAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RHQLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKQPQAAEAEEK
                     SLETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVT
                     KDHILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQAGKVMLDS
                     VPVDPNMLNQFQITMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALS"
     gene            complement(13073..14212)
                     /gene="flhB"
                     /locus_tag="ACHJ8C_003268"
                     /pseudo
     CDS             complement(13073..14212)
                     /gene="flhB"
                     /locus_tag="ACHJ8C_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236920.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            complement(14202..14984)
                     /gene="fliR"
                     /locus_tag="ACHJ8C_003269"
     CDS             complement(14202..14984)
                     /gene="fliR"
                     /locus_tag="ACHJ8C_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO5553074.1"
                     /translation="MRTSDVTQLMDLALGLWFPFVRIMAFLRYMPVLDNSALTARVRI
                     ILSLALAIIITPLIPHPIPHDLLSLNSLILTVEQILWGMLFGLMFQFLFLALQLAGQI
                     LSFNMGMSMAVMNDPSSGASTTVLAELINVYAILLFFAMDGHLLLVSVLYKSFTYWPI
                     GNALHPQTLRTIALAFSWVLASASLLALPTTFIMLIVQGCFGLLNRIAPPLNLFSLGF
                     PINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            complement(14986..15258)
                     /locus_tag="ACHJ8C_003270"
     CDS             complement(14986..15258)
                     /locus_tag="ACHJ8C_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HFO5553075.1"
                     /translation="MLTVDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGVCGKWMIVQLHDLCIHLFTQAALLVH"
     gene            complement(15261..16013)
                     /gene="fliP"
                     /locus_tag="ACHJ8C_003271"
     CDS             complement(15261..16013)
                     /gene="fliP"
                     /locus_tag="ACHJ8C_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538309.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO5553076.1"
                     /translation="MKRRTQLTLGLALLALAPLALAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIALSLTLL
                     VMRPIWLNIYDHAVVPFENDQITLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GNAADQDLSIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            complement(16010..16381)
                     /locus_tag="ACHJ8C_003272"
     CDS             complement(16010..16381)
                     /locus_tag="ACHJ8C_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO5553077.1"
                     /translation="MSKQEDILAGDFGLTDEVAPVAKSADAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            complement(16374..17225)
                     /locus_tag="ACHJ8C_003273"
     CDS             complement(16374..17225)
                     /locus_tag="ACHJ8C_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO5553078.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPTLFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAELMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKAHNGLPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVITHWAYQLTFT
                     LAGDEESSFRILLDDAHTDFILNLIRHSEHSKPQQVAKSVNKPALIKEIIRSMPLTLN
                     VKIAELSLNVADLAQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAG
                     KNENNHE"
BASE COUNT         4210 a         4658 c         4493 g         4088 t
ORIGIN      
        1 aagcaaactt catatttgat aaggtatatt catgagcaca acataccaat gtatcgtcag
       61 gtaacgcact taactttcta agtgattgat acatttgtga tggtgtccct tcaaacaacc
      121 gaccacaccc accagaaaac agcgtgtcgc cgcaaaatag ataaggttta ctgaagtaac
      181 agatatgtcc taaagtgtga cccggtgtgg caattacact aaattcatgc cccaaaacga
      241 aggcagtttc gccatctttg actacctgtg ttgttccctt atcttgtgtc tcttgtggtc
      301 catacaccac aatttgcgga aacttttcca ccagttcttt tacgccgcca acgtgatcgt
      361 ggtgatggtg ggtgagaaat atggcctccg gttgccagtt attggcggaa atggcgttta
      421 atactggctc tgcgtccccg ggatcgacaa tcagacagcg acctgcttca tcattcaaaa
      481 cccagatgta attgtcatca aaggcgggaa tactgttaag attcatagat tacctctcag
      541 tgtgaaacgg aaggttgtga tgaaaccggc aagagtccct caaactgtcg tggctcctga
      601 ttgctggggc gatttgccct ggggagagct ttatcgcaag gcgctggagc gtcagctcaa
      661 cccgtggttc actaaaatgt atggttttca tctgcttaag attggcaatt taagcgcaga
      721 aatcaattgc gaagcgtgcg cggtttctca tcaagtgaat gtttctgcgc aaggaatgcc
      781 cgtccaggtg caggcggacc cacttcatct tccttttgcc gataaatccg ttgatgtttg
      841 tctactggca catacattgc cgtggtgcac tgatccgcat cgtttattgc gtgaagccga
      901 tcgggtactg attgatgatg gttggctggt gattagtggc ttcaatccca tcagtttaat
      961 gggattacgc aaacttgtgc cggtattgcg caaaacatcg ccctataaca gccggatgtt
     1021 tactctgatg cggcagctgg actggctctc tttgttgaat tttgaagtgc tacacgccag
     1081 ccgtttccac gttctcccgt ggaacaaaca cggaggaaaa ctattgaatg cgcatattcc
     1141 tgcgcttggt tgcttacaac ttattgttgc ccggaaacgg actattcctt taacgctaaa
     1201 tccgatgaaa cagagcaaga ataaaccgcg aattcgccag gcggttggtg ccacccggca
     1261 atgtcgtaaa ccacaggctt aaacttcgac ttgatagcct gtatcttcca gtgtgggatt
     1321 cattgccgca gcacgagcca gttcatcaca gcgttcgttt tccgggtgtc cggcatggcc
     1381 tttaacccat tcccatttga tttgatgctg ccccagagca gcatcaagac gttgccagag
     1441 atcgacattt tttactggtt ttttgtctgc tgttttccaa ccacgttttt tccagttatg
     1501 gatccactgg gtgataccct ggcggacata ctggctgtcg gtactcaaaa tgacttcgca
     1561 atgttctttt aacgcctcca gcgcgacaat agcggccatc aactccatac ggttgttggt
     1621 ggtgcgggtg tagccagcgc taaaggtttt ctcgtgtccg cgatagcgta aaatagcgcc
     1681 gtaaccccca ggtccaggat tacccagaca cgaaccatcg gtgaaaattt ctacctgttt
     1741 aagcatctct ggtagacttc ctgtaattga atcgaactgt aaaacgacaa gtctgacata
     1801 aatgaccgct atgagcactg caattacacg ccagatcgtt ctcgataccg aaaccaccgg
     1861 tatgaaccag attggtgcgc actatgaagg ccacaagatc attgagattg gtgccgttga
     1921 agtggtgaac cgtcgcctga cgggcaataa cttccatgtt tatctcaaac ccgatcggct
     1981 ggtggatccg gaagcctttg gcgtacatgg tattgccgat gaatttttgc tcgataagcc
     2041 cacgtttgcc gaggtagccg atgagttcat ggactatatc cgcggcgcgg agctggtgat
     2101 ccataacgca acgttcgata tcggctttat ggactacgag ttttcgttgc ttaagcgcga
     2161 tattccgaag accaatacct tctgtaaggt caccgatagc cttgcggtgg cgaggaaaat
     2221 gtttcccggt aaacgcaaca gcctcgatgc gttatgtgcc cgctacgaaa tagataacag
     2281 taaacgaacg ctgcatgggg cattactcga tgcccagatc cttgcggaag tttatctggc
     2341 gatgacaggt ggtcaaacgt cgatggcttt tgcgatggaa ggagagacac aacagcaaca
     2401 aggtgaaaca acaattcagc gcattgtgcg tcaggcaagt aagttacgcg ttgtttttgc
     2461 gacagatgaa gagcttgcag cacatgaagc ccgtctcgat ctggtgcaga agaagggcgg
     2521 aagctgcctc tggcgggcat aaatacctgt aaaaggcgct aaaaatagcg acttgggcga
     2581 tttttgcagc aaacgattca aaagatgaga aaaaccgttg acgaagggtg aggcaatccg
     2641 taatattcgc ctcgttccca acggaacaca acgcggagcg gtagttcagt cggttagaat
     2701 acctgcctgt cacgcagggg gtcgcgggtt cgagtcccgt ccgttccgcc aatattcaga
     2761 aagcccgaat cattactgat tcgggctttc gtcgtttaag ttccagagaa aaatagccac
     2821 gaaacttatc tcgtagccat attcaccctc taacgaaatg tgaactcatc cgcatcctgc
     2881 catgccggaa acttttctct atattcccgc aatgccatca tcgatagctc cgcatcaatg
     2941 cgcgttgcct gatgcgtgtc ggcagtagcg ataatctcgc cttgtggatt aatcacccgg
     3001 ctgtcaccgc gataatggca gccgttgcca tcgctgccga cgcgattaca ccccgctaca
     3061 tacgcctgat tctcaatcgc gcgggctgtc agcaatgcct gccagtgcag agagcgcggt
     3121 gcaggccagt tggcaacgta gatggcgagg tcataatcgt tgagattgcg cgaccacacc
     3181 ggaaaacgta agtcgtagca caccagcggt aaaatacgcc agccgcgcca ttccacaatc
     3241 actcgctcat tgcccgcttt ataatgcaga tgctcatctg ccatgcggaa cagatgacgc
     3301 ttatcataaa aatgtaccgt gccgcccggc tcaaccagca aaaagcggtt aaccgaatca
     3361 gactccgttt gtaatgcaac actgccagca atcagcgcat tgcactgctg cgctttagcg
     3421 gtcatccagt tcactacgtc atcttgtgct agcgacgaag ctgccgcttc catggcaaag
     3481 ccgctggtaa acatctccgg tagaacgatc acatcgcgcc cggtaatacc ttccagttga
     3541 cgatcaaaat ggcgcaggtt ggcaggacca tccatccaca ccagtggttg ctgcaaaagc
     3601 gtaatcttca aaccaggcac ggtgtacaac tcctttatgc gaagggtttt ataactctaa
     3661 caccttatca ggcagttgcc ttagcgcaga ataaattgat aacaaatgct gatattggaa
     3721 atatctgatt tgcaaattat cgtgttatcg ccaggcttta gggagttaat cacatgggca
     3781 ggataagccc gggaggaatg atgtttaagg caataacgac agtcgccgct ctggtcatcg
     3841 ccaccagtgc aatggcgcag gatgatttaa ccattagcag tcttgcaaag ggcgaaacca
     3901 ccaaagcggc gtttaatcag atggtgcaag ggcataagct gcctgcctgg gtgatgaaag
     3961 gcggtactta tactcccgca caaaccgtga cattgggaga tgagacgtat caggtgatga
     4021 gcgcgtgcaa accgcatgac tgtggctcgc aacgtatcgc tgtgatgtgg tccgagaaat
     4081 ctaatcagat gacggggctg ttctcgatca ttgatgagaa aacgtcgcaa gagaaactca
     4141 cctggttgaa tgtgaacgat gcgctttcta ttgatggtaa aacggtgctg ttcgcggcgt
     4201 tgaccggcag cctggaaaac catccggatg gctttaattt taaataatta gccgataaaa
     4261 aaacggagcc gaaaggctcc gttattcaat tacgcggctt caactttccg cactttctcc
     4321 ggcaacttta ccggcttcgt cgccaggtct tctggatcaa agtcatcaac gttaatacta
     4381 cgcagacggc tttcttcagc ttttaccaga atagcggctt catctttatc aatcagcccc
     4441 ttcgccagcg cgttgtgcgc cagttcatcc agacgggtaa acggcaggtt tttacccaac
     4501 tctttacaga tccgctgatg aatcgggtcg gcggcaatca catccaccag cgcctcttcc
     4561 agtaaaccaa ccgggttatg cttgctcggt gtcaggtact gaccgcgacc aatacgtgaa
     4621 cgggtggcgt tcggcacttg taaaatcttc gccaccttat gatccagctt gtcagacggt
     4681 gccagataat gacgtccggt cgggaagatc accacattca gcagcccggc aaccacgcgg
     4741 ttcgggaagt tttgcagtaa atcgtccatc gcctgttctg cctgatacag cgcatcctgt
     4801 acaccccagt gcaccagcgg caggtcggct tcattacggc cttcgtcgtc ataacgcttc
     4861 agaacggcag aggcgaggta aagctggctt aaaatatccc ccagacgggc cgagatgcgc
     4921 tcacgacgtt tcaggctgcc gcccagcacc gccatcgaga catcagaaag cagggcgagg
     4981 ttggcgctca ggcggttcag gtgctgatag tagcgtttag tggcatcgcc ggttggtgtg
     5041 ctgctggtta aaccgcgcgt caggcccagc cagaagctgc gaactttgtt actgccgacg
     5101 tggccgatat gtttgaacaa cagtttatcg aacgcgttga cgtcattgtt cttcgccgct
     5161 tccatctctt ccagcacgta cggatggcaa cgaatcgccc cctgaccgaa gatcatcatg
     5221 ctgcgggtca gaatgttggc cccttcaacg gtgatggcaa tcggtgcccc ctggtaagca
     5281 cgtgccagga agttgctctg accgagcata atgcctttac ccccggtgat atccattgcg
     5341 tcgataatcg actgctgccc gcggtgggta cagtgatact taacgatagc cgacagtacg
     5401 gcgggttttt caccgagcat aatgccgtag gtaatcagcg atgccgcagc atccatcacg
     5461 taggcgtttc cggcaatacg cgccagcggc tcttcaatcc cttccatctt accgatagag
     5521 attttgaact gacggcgaat gtgcgcatac gcgcccgttg ccagcgcaac cgatttcacg
     5581 ccgccggttg agttggaagg cagggtgatg ccgcggccta ccgagaggca ctccaccagc
     5641 atccgccagc cttgcccggc cattttcggc ccgccgatga tgtaatcgat cggcacgaac
     5701 acatctttac cgcgcgtcgg tccgttctgg aacggtacgt tcagcgggaa gtggcgccga
     5761 ccaatttcca cgcccggcgt ggtggtcggg atcagcgcac aggtaatacc taaatcttct
     5821 gcaccgccga gtaatttctc cgggtcggag agtttaaacg ccagaccaag cacggtcgca
     5881 atcggtgcca gcgtaatgta gcgtttgttc caggtcaggc gcatccccag cacctgctgg
     5941 ccctgccatt cgcccatgca gacaatcccg gtatccggaa tcgcgcccgc atcggaaccc
     6001 gcttccgggc tggtcagtgc aaagcaaggg atctcctgac cacgcgccag acgcggcaga
     6061 tagtgatttt tctgctcgtc agtgccgtaa tgttgcagca gctcgcccgg gcctaatgag
     6121 ttcggcacgc cgacggtaat cgccaggatc ccgctcacgc cggagagttt ttgcagcacg
     6181 cgagactggg cataagccga gaactccagc ccgccgtact cttttttgat gatcatcgcg
     6241 aagaaacgat gctctttaag gtacgcccat aactccggcg gcaggtccgc cagctcatga
     6301 gtaatctgga aatcattcgc aatccggcag gcttcttcta ccggaccgtc gagaaacgct
     6361 tgctcttcag cggtcaggcg cggctgcgga tagttatgca gctttttcca gtccggcttg
     6421 ccctggaaca agtcgccctc ccaccaggtg gtgcccgcat caatcgcttc tttctcagtg
     6481 cgcgacatcg gcggcatcac cttacggaaa ccgcgaaata ccggtgcgga aatcatcgac
     6541 ttacgcatag gcgcaaagtt aaatggcacg aggataatgg ccagaggcac tagtacccac
     6601 gccgaccaca gaccagcaac gccgaggacg gctgtccagg cgagcaaaat cagactgctg
     6661 ataaataagc tcacgcggtg atagaacaat gcgccgagca ggacaaccgt agcgagaata
     6721 ctcaaaatca tcataacgaa aagcccctta cttgtaggag gtctgaccac ttgtgatgat
     6781 atggttgtag tggatgtaaa aacatttagc aatgtgttta caatataatt acaacaaagc
     6841 tcacattgtt gctgttttta tccgcacttc aggtcaaaaa gtcctggtca tagcacctgc
     6901 ccgtacttct cgcttttggc ggtatccggt acactgcatt ttgtctatta catttatgct
     6961 gaaggatatc ctcatgtacc aggatcttat tcgtaatgaa ctgaacgaag cggcggaaac
     7021 gctggctaac tttttaaaag atgacgccaa tattcacgcc attcagcgcg cggcggtcct
     7081 gttagcagac agctttaaag ccggtggcaa agtgctttcc tgtggcaacg gcggctccca
     7141 ttgcgacgcc atgcactttg ccgaagagtt gaccggtcgc taccgtgaaa accgtccggg
     7201 ctacccggcg attgccattt ctgacgttag tcatatttcc tgcgtcggta acgattttgg
     7261 tttcaatgat attttctccc gttacgttga agcggtaggt cgcgaaggcg atgtactgct
     7321 ggggatctcc acttccggta actctgcaaa cgtgatcaaa gcgatcgcgg cggcgcgtga
     7381 gaaggggatg aaagtgatca ccctgaccgg taaagacggc ggcaaaatgg ctggcacggc
     7441 ggatatcgaa attcgcgtac cgcactttgg ttatgccgac cgcattcagg agatccacat
     7501 taaagtgatc catatcctga tccagttaat tgaaaaagag atggttaagt aaggtctttt
     7561 ctccctttca gggagagggc cggggtgagg gtaacagcct gaacctttgt actttttaat
     7621 caatcttcgc cgggggccat gcgctcccgc tgttgtggag gttacccatg tgcgaattgc
     7681 tcgggatgag cgccaacgtc cctaccgata tctgctttag tttcacagga cttgtacagc
     7741 gtggtggtgg aaccgggcca cataaagatg gctggggcat taccttttac gaaggtaaag
     7801 gctgtcgcac atttaaagat ccgcaaccca gctttaattc ccccatcgcc aaacttgtcc
     7861 aggactaccc gataaaatcc tgttcggtgg tggctcatat tcgccaggcg aatcggggcg
     7921 aggtggcgct ggaaaatact cacccgttta cccgcgagtt atgggggcgt aactggactt
     7981 atgcccataa tgggcaactg acgggctata aatcactgga aaccggcaac ttccgcccgg
     8041 tcggtgaaac cgacagcgaa aaagccttct gctggctcct gcataaatta acgcagcgtt
     8101 acccgcgcac accgggcaac atggcggcag tgtttaaata tatcgcctca ctggcggatg
     8161 aactgcggca gaagggcgtt ttcaacatgc tgctttctga cgggcgctat gtaatggcgt
     8221 attgctcgac taatttacac tggatcaccc gccgcgcgcc atttggtgtg gcaacgttgc
     8281 tggatcagga tgtggaaatc gacttcagct cgcagaccac accgaatgat gtggtcacgg
     8341 tgattgcgac acagccgctg acgggcaatg aaacctggca aaagattatg ccaggcgaat
     8401 ggcgcttatt ttgcctcggg gagcgtgtag tttgatgcca gctgtggttg cacaacttcg
     8461 tggcttaacg gcttgctgac cacgtaacgg ccattgacca cagaaacggt cggtggctta
     8521 cgggtttgct caaagtagtc gtagcccggc ttcagttgct cccagaagtc cttaaagttg
     8581 gaatatttat ggcgcttcat attggcgtcg gtcatgcgga acgggtaaat actcacttgc
     8641 acgctcggct gaccaaacac cagcgcacca gtaacgaact ggaagatctc atcaataccc
     8701 tgattggtca tcgcgtagca gccgatggaa acacaatcgc cgtggatcat caggtatttc
     8761 ccttcataac catgcgcacg gtcataggca ttggggaaac cgatattaat cgccttgtag
     8821 taacggctgt ctggttttaa ctgattacgc tggacgctat aaaacccttc cgggcttttg
     8881 aaatcgccct gacgctgttt tggccccaag ccgccggaat atttacagat tttatagctg
     8941 tcgagcagtt gatattgctc gcccattttg acgtagagat cgagcgtacg ttcttccttg
     9001 aagatctgga tgtagacagg ggatcccatc aactgctgct tatactcttt gctcactggc
     9061 gtggtcgagc tactgctgcc cagcaaaccg gcaaacgaaa cgcacgggat caacaacatc
     9121 gcaagaatta atgcgatttt acgcatactg cttattcctt gataaaacgg ttacacacgc
     9181 caggacggca aaatggatcc caaatcggaa tagtctggat tgggaaggct cacattatca
     9241 ccaaaagagt tttacgcaag cctgtcggcg cagggtttac aaatttagca caggggcagt
     9301 tttcaagagt ccgaaaagcg catggattgt agatttattt cttatttcct aagattatga
     9361 aaccgcaata gtaatatgca aataagcaat taataatccc gtttactcct ccggtctttt
     9421 aaaattctca ccttcattca gctggtttat gtccttcatg tcttcgttct tgcacggcag
     9481 atttctgcat ccaggcgtgt tttcgttatg cgttttgctc cctttactcg ccagcgccac
     9541 cacatcacat atctctttta gctacgccgc ccgccagcgg atgcaaaacc gtgcgcgttt
     9601 attaaaacag taccaaaccc atctgaaaaa gcaggccagc tatattgtgg aaggcaatgc
     9661 caaaagcaga agggcgctgc gccagcataa tcgggagcag ataaaacagc atccagaatg
     9721 gtttcctgcg ccgctcaagg cgagtgacag acgctggcag gcgttggtgg agaataatca
     9781 tttcttaagc agcgaccatc tacataacat caccgaagtg gcgattcacc gcctggagca
     9841 gcagctaggt aagccttata tctggggggg cacgcggcct gataaaggct tcgactgtag
     9901 cgggttggtt ttttatgcct acaacaagat ccttgaggct aagctcccgc gcacggccaa
     9961 tgagatgtac cactaccgtc gggcaacgat tgtggcgaat aacgacctgc gccggggaga
    10021 tttgctgttt ttccatatcc acagccgtga gattgccgat catatgggcg tgtatctggg
    10081 agacgggcaa tttatcgaat cgccacgcac gggggaaacc atacgggtaa gccggttagc
    10141 cgaacctttc tggcaggacc actttttggg tgcgcgcagg attttgacgg aagagacgat
    10201 tttgtaaatg ggtaatattg acgaactggc aaaaggtcgg atgccacgcg gcatccgacc
    10261 tgtttacatc tgggctgaaa actcacgttc taagacatca ataatgagct ggttttttga
    10321 aatggaatgt tgtctggcga cgatggtcag ccgcgaatca atgtgatgag gaacacgaag
    10381 cgtcaggcgt ttttgctctt cttcttcacg aaaaggggca ataccgtcct cttcacaatc
    10441 cgccatccac tcatccagcg cgctttcgcc ttcacgatat aatgcctcaa tacttgtgga
    10501 tacaaagtca caataaccgt ttacattgag gaacttgccg cgaaatgcgt tgatatcggc
    10561 ttcaaatgtc actgcggctg gttgaccttt aattgtgata atgttcgatg ctttattcat
    10621 aatctacccc tacacagtta acaaaccact ccttaagacc tgctactgcg cctttatcca
    10681 tcacattctt tggatgtggc tcatgtgtgc tgaatttggt cgttccaatc tgaaattttc
    10741 gtctggagcc gttgttattt tttattgttc cacccacagc tttaatgagt gcaacaacat
    10801 catcccattt aattccagat tttggcggct ccgtcattac atcgtcgaat gtttttctat
    10861 gacgagagcc tagcgcgctg accttaatca tactatcaat ccatgacgtc attgtatggt
    10921 gtcatcgtag tctctttgca gtgtgacgtc aattaatggt gtcaggtgct gcaaagcatg
    10981 gaaaagattc ctcaatcaac tcaacgcccc cataatcttc aactccagct cgtccggcac
    11041 ttcgttatac gacagcacat gcagccccgg cgcaaacaac cttgcgtagc gcgccagcaa
    11101 ggggcgcagc tgcggcggca ccagcagcac tggatctttc cccgccgctt tcatctgttc
    11161 cttcacctgc ggcatcgtga tctgaaactg gttgagcata ttcgggtcca ccggcacgct
    11221 atcgagcatc actttgccgg cctgttgcgc ctgattcacc acgttggtca gcagattttc
    11281 cagctcatta ttcagcgtat acaccgtcag ctcctgcttg cgaacgaacg gatgggttat
    11341 gctgcgccgc agcgccaggc gcacatcggc cgccagcaga atatgatctt tagtcaccgc
    11401 actactggcg accaacacgg tggcgatggt gacgatatcg cgcagggaaa cgccttcagt
    11461 cagtagcgcc cggtacactt tcagcaactg gctgtaattg agcgccgcgc tcaaatcttc
    11521 cgccagtcgc ggtgccatcg acgacaaacg gttatgcagc tgagtaatat cgtcatagtt
    11581 aaacaaatca ggaatatagc tgcgcacaat cttattcaca tgggtggcaa ttacgctggc
    11641 gctgtcgatc acctgatacc ccatattcag cgccttcgct ttctgcgcgg gctgaatcca
    11701 ggtgaccggc atcccgtacg ccgggtcgtt ccccagcacg ccgtctatct cgccgtaggt
    11761 ttcgctggag ggcagggcca tcagtttatc tgccggaata tccgcttcat cagccttaat
    11821 gccgttgatg aaaatggcgt actggctggg cttgaggcgg aagttttccc gaatgcggat
    11881 ctccggcagc agcacgccgt tgccgtcaga aatcacctgc cgcacgccgc gaatccgctg
    11941 ggtgagcggg ttaccctgtg ctttgtccac cagcgccacc agcttgtagc cgaggcttaa
    12001 actgatgggc tcgatcagcg gaatggtttc ccaactgacc tgttgctcgc tggtttcggt
    12061 gatggttcgg gtcagcgttt cgaggctttt ctcctccgcc tccgccgcct gcggctgttt
    12121 gctcatccgc cagccggtaa agccaagcag ggcgctgaac agcaaaaacg gcaggtgcgg
    12181 catccccggc accaccgcca gcacaaacat aattccggta gcggtgtaga gcaccgacgg
    12241 gcttgccagc aactggtggc gcacgtcatg ggcaatatcg ccattatcgc tgacgcgggt
    12301 gacgataatc gccgctgcgg tggagagcag cagggaaggg atctgtgcca ccagtccgtc
    12361 gccgatggtc atcaacacat attgctggaa ggcggcgtcg gcgctcaggt tgtatttgaa
    12421 gatcccgata cagacgccgc cgatcaggtt gatcgccaga atcatcatcc cggcgatggc
    12481 gtctccgcgc acaaacttcg atgccccgtc catcgcaccg tagaaatcgg cctcgctggc
    12541 aacatcttta cgccgggttt gcgcctgcgc ctggttgatc aatccggcgt taagatcggc
    12601 gtcaatcgcc atctgtttgc cgggcatcgc gtccagggta aagcgggcag aaacctcgga
    12661 aatacgctcg gcccctttgg tgacgacaat aaagttgatg atcatcagga tgataaacac
    12721 cacgaaaccg acgacaaagt tgccgccaat caccacctga ccaaatgact caatcacctt
    12781 acccgccgcg cccgcgccga gatgcccgtg cagcaacacc acgcgcgtag aagcaacgtt
    12841 aagcgtcagg cgcattaagg tggtgatcag caaaatggtc gggaacaggc tgaattccag
    12901 cggacgtttc gccgacacgg ccaccagcag caccatcacc gccagcacaa tgttgaaggt
    12961 gaacagaata tccagcagcg caggcggcag cggcagaata accatcgcca gaatacacag
    13021 gatcaccagc ggcacgccga gattgccgcc gcgcagtagc gcgaggaacg atttagttgt
    13081 tttcgccatc cagtttaagc acctctttgg gaatggaaat atgtctgttc agggccgggc
    13141 gcggctgtgc gccctcgcgc cagtgtttca tttgcaggac gtaggtcaga acgtgggcga
    13201 tggcccggta aagctgaaac ggaatttgct ggttcacctg ggtggtgtag taaaccgagc
    13261 gcgccagtcg cgggaactcg accacttcca cctgattctc agtggcgacc tagcgaatat
    13321 agagggcgat ttcatcggtg cctttcgcca ccacaaacgg cgcggcggcg cgcgactggt
    13381 catactgcaa cgccacggcg tagtgggtcg ggttggtgat caccacgttg gctttcggca
    13441 ccactttgcg aatttgcccc atcgccagtt gccgttgcag ccgccgcacg cgcgccttca
    13501 cttccggctt gccttcctga tttttgtact cttctttcag ctcctgtttg gtcatcttca
    13561 gccctttggt gaacagggct ttcgccagcg gcacgtcgat taacgcaaag aagataaaca
    13621 ggatgacaaa gttgcgcatc acgctggcgt agagcgataa cccatcattc atcgccagtt
    13681 tgaacggcag tgcctgcagg ccgagaaagg cggcaaagtt attgcgcagg ctaatccaca
    13741 gcatcaccag caccaccacc gacttcactg tcatctttcc cgtttccgcc agatgctcac
    13801 tgaaaaacag tcgcccaatc ccttttaacg ggctaatttt gctgaaatcc gggaggattt
    13861 ttttcggcaa gaacagccag ccgccgggca ccagcgagga gagcagcgcg gcggcgggca
    13921 tcggcagcaa ggtaagaata aacttgccga gaattaacag gtgatggcgc agaaactggc
    13981 cgatcacatc cgggttatta atctcctgcg cgtactgatg cacgctgata aaactctcgc
    14041 gcacaaaatc ggcataccac gggaagctgc tggagatcac cacaaacgcc gcaaacaggc
    14101 tcgccgccag ccccatatcc ttcgagcgcg gcagttgccc ctcttcgcgc gctttacgca
    14161 gcttttgcgc cgagggtttc tctgtttttt cttcgctgct gctatccgcc atagtgacct
    14221 ttcagcgcgt cgagttgctg caacacaaaa ttcgccagat gcagatagtg atccggcagg
    14281 ttgtagagca gggtggcgaa gcagaccagc cctgccagca tgttgatggg aaagccgagc
    14341 gaaaaaaggt tcagcggcgg cgcaatgcgg ttgagcaggc caaaacagcc ctgcacaatc
    14401 agcatgataa aagtggtcgg cagcgccagc agtgatgcgg acgccagtac ccagctaaag
    14461 gccagcgcga tggtgcgcag ggtttgcgga tgcagcgcgt tgccaatcgg ccagtaggtg
    14521 aagctcttgt acaacacgct taccagcagc agatgcccgt ccatggcaaa aaatagcagg
    14581 atcgcgtaga cgttgatcaa ctccgccagc acggtagtcg atgcgccgct gcttgggtcg
    14641 ttcatcaccg ccatgctcat ccccatattg aacgagagaa tttgcccggc cagttgcagc
    14701 gccagaaaca gaaactgaaa catcagcccg aataacatgc cccataaaat ttgctctacc
    14761 gtcaggatca ggctattgag cgaaagcagg tcgtggggaa tgggatgggg gatcaacggc
    14821 gtgatgatga tcgccagcgc cagcgacaaa ataatccgca cgcgcgcggt cagggcgctg
    14881 ttatccagca ccggcatata gcgcagaaac gccataatgc gcacgaaggg aaaccacagc
    14941 cccagcgcca gatccatcag ttgcgtaacg tcgcttgtgc gcatcttaat gaaccagcag
    15001 ggcggcctgg gtaaacaggt gaatgcacag gtcatgcaac tggacaatca tccattttcc
    15061 gcacacgccc agcaccacca gcgtgacaat cagccttggc aaaaagctca gggtctgttc
    15121 attaatctgc gtcaccgcct ggaacacgct caccagcagg ccgaccagca ggcttggcac
    15181 caccagtacc gaaaccagca aaatcactac tttaataccg ctggcgacaa tgtcggcggc
    15241 tacgtctacg gtcaacataa tcagcccagc cccagcccgc gaatgctggt ggtgagcgtg
    15301 ccgatggtca gcgaccagcc gtcaatcagc acaaacagca tcagtttaaa gggtagcgaa
    15361 acaatcagcg gcgacagcat catcatcccc atcgccatca atacgctggc gacaatcagg
    15421 tcgataacca gaaacgggat gtaaatcata aagccaatct ggaaggcggt tttcagctcg
    15481 ctcagtacat aggcgggcac cacgatggag agatcctggt cggcggcatt gcccttcgcg
    15541 ccgccgatgg tcatgatttg cgccatcgct tttttgtcgg tctgcgccag cataaagcgt
    15601 ttcagcggcg tcgcggcggt gcttaacgca tcggtcaggg taatctgatc gttttcaaac
    15661 ggcactaccg cgtggtcgta aatattgagc cagatggggc gcattaccag cagggtgagt
    15721 gagagcgcaa tacctatcag aatgcggttc ggcggcgttt gttgcagccc cagcgcctgg
    15781 cgcagcagcg acagcacgat aataaagcgc gtaaagcagg tcatcatcag caccatcgtc
    15841 ggcagcaaac cgacgagggt catcagaatc agtacctgaa ttttgacgct gtactcctga
    15901 gtgttgccgt gggtcaccac gttaagcagc gcaatatcgc caccctgggc cagcgccagc
    15961 ggggcgagcg ccagcagcgc caggcccagc gtcagttgag tacggcgttt catggtgcca
    16021 ggtcgttgat atcgcgggtg ttaaattcca gcacgcgcag gccgtacttc tcgttgacca
    16081 ccaccacttc cgctttgccc agcaggatat tgttcacttt aatatccagc ggctccccgg
    16141 cgagtttatc cagttcaatc accgtgtcgt cgtcgatgtt aagcaggtcg gctaacatga
    16201 tttccaccga cgacacttcc agcgttaagg tcaccggaat gcgtttcagt agcgtcatcg
    16261 agtcggaaaa tcggtcttcc aggcgggtga ccagcgtttc ggcgtcggcg gatttggcga
    16321 caggcgcaac ttcatcggtg aggccaaaat ccccggctaa aatatcttct tgcttactca
    16381 tggttgttct cattttttcc cgccagctcc gacaaaaaca gtttgtcttt gtcttccaca
    16441 atcagggcgc taaataattc agattgccca atcgccaccg ggaacgtttc tcccagcgag
    16501 atgggtaaaa tatcccccgc ttttatctgc gccagatccg cgacattcag cgacagctcg
    16561 gcgattttga cgttcagggt cagcggcata gagcggatga tttctttaat cagggccggt
    16621 ttattaacgg atttcgccac ctgctgcggt ttactgtgct cgctgtggcg gatcagattc
    16681 aaaataaaat cggtgtgggc gtcgtcgagc aaaatacgaa aactgctctc ctcatcgccc
    16741 gcaagggtaa aggttaattg atacgcccag tgggtaatta ccgtgctgct gtccagtttt
    16801 aatttcagcg caataccgga ggtttgcaga ttaaatatca gcgtgcagat atccagcgcc
    16861 agacggttct ttaatcgtgt ttcggttttg gtcggcaacc cgttatgggc tttagcctct
    16921 tcgagagacg actccagacc ataaaaattg cccagcagca ttaacaataa ggagcgatca
    16981 atatcaaacg ccagatgccc gacctgggaa accatcagtt cagcgttttt attaataata
    17041 tccatttcgc agccaatctt ttttagggtg atattgctgc gatgcttttt gagaaagtag
    17101 ctgccaagat gcgaatcaat tacgtcaaaa ttccccgtaa acagggtcgg caggcgatgg
    17161 taggggcgtc caagccgatt accttctaat ttaaagatgc ctggcgtttt actgtacttc
    17221 agcatggctt actttctctg ctgtaatggt atgggcgatt ttgtgcacgc aaatccggcg
    17281 gtgacacaag gtgaggtatt aaatagaggg ggagtgaaag gggcaaatta ttaatagggt
    17341 gaaaaaataa ttaaattatt aacgggcgag atatcgggca tcgtcataaa acattgccca
    17401 aactgtaggc ctgataagac gcgcagcgtc gcatcaggca tcggcacgc
//
LOCUS       DAZFJL010000099        17306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFJL010000099 DAZFJL010000000
VERSION     DAZFJL010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.099"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            13..531
                     /locus_tag="ACHJ8C_003274"
     CDS             13..531
                     /locus_tag="ACHJ8C_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO5553079.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            547..1086
                     /locus_tag="ACHJ8C_003275"
     CDS             547..1086
                     /locus_tag="ACHJ8C_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO5553080.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1181..2758)
                     /gene="guaA"
                     /locus_tag="ACHJ8C_003276"
     CDS             complement(1181..2758)
                     /gene="guaA"
                     /locus_tag="ACHJ8C_003276"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO5553081.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2827..4293)
                     /gene="guaB"
                     /locus_tag="ACHJ8C_003277"
     CDS             complement(2827..4293)
                     /gene="guaB"
                     /locus_tag="ACHJ8C_003277"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO5553082.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLSQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4455..5831
                     /gene="xseA"
                     /locus_tag="ACHJ8C_003278"
     CDS             4455..5831
                     /gene="xseA"
                     /locus_tag="ACHJ8C_003278"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO5553083.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQLKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANLRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPIVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     THQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQLRLTRQLVQQNPQSRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKVGETLTTRLGDGVVISEVSAVTKTRK
                     SRKKTSNP"
     gene            complement(5900..13951)
                     /locus_tag="ACHJ8C_003279"
     CDS             complement(5900..13951)
                     /locus_tag="ACHJ8C_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF823 domain-containing adhesin"
                     /protein_id="HFO5553084.1"
                     /translation="MSLTLNKILVLCALLISAMLPGWSWAESAWQDSSDTVGEFNGTV
                     PTADSASIPVYQGSVFLDPTKTHEVAFTAKPSEFNADVSVSKLLVTNPQDREGDIIAT
                     PRWENQTPPAVSLVWADAATPDTLLDPQPVADRSFCAQGLAGRSLVAWAQPDPQQTMP
                     LLYLLTSTGYPYESVLTLADQKVTLKIAPAQGDLISVSAAGYDESSGAAKMTVGGSIT
                     LTVTTKDCVGNVVGNIPFVIKRKDAENRQGVVNNTAPVKLGTTELTTTATEYRGTTDA
                     NGVATVTVTQANGPGVKTPLVASLAGIAQASETAVIFTVLTSPDVPQATMWGHMPDTL
                     KARDYTFSRPKLAAEVDNEDGTVNDHNETWSTFTWSGADKHCDILPGMRQFGALATVV
                     PTSVQDVAGWPMQGNFYWSSLAGMSGQHHAVDVSNRSEAQKPDDTTFIVSCVDKEAPD
                     VEPKLVLTPGSYDSTIKAMKVKVGEEASLRLTITDSKNNDQPLAYYYFSLHLDDGINR
                     KNQTDAAWETDPVQIDGGSNVRKVDAHTYEGITDANGEATLTLTQPGGVGVKTHITAR
                     MRSDFTASDEKDVIFTVITSPDTDKARMWGHMLGIIEANNIFKRPRLADETDNELGSV
                     RENNEDWALFDQNSSMQAECGLGHIPSQSSLHSLFAAHPANSIGTEYGWPTLQKAYLS
                     AVEETSHASVNLATGNIDTYSGFKQNYLSCSGNEMVAQIAATTDRDVSAGSRAQAKVG
                     DTITMTVRTFNALNNAPVPYTAFTITKDMGKNRQGQTTGFDDPTRGAIEMNGTLYGTS
                     QPSLVYAGTTDAQGFATVEIKQSQGVGLSTPLNIVPVNSYIPNTVNYNVIFTTLTSPD
                     AVGAQMWGHMDETITVDALTFARPRLAAEVFSPDGTLTENNEVWSRVSQANASSTSKG
                     GCGANMLPRRSQLSALYDANNGNGVQTVHGWPTQRQPYWSSSPADQVPHYYTIALNDG
                     ARTVGGSTAVYVSCLTTANNPASSITLEVVDPAQWNAAANAAKLKKGEMLQVKVTVKD
                     AQGNPLGDMPFTLKRGDGYTRSNEKHIAGSGDALVAPVVINSGLADETLLNDTAAAYS
                     AMTGSDGTKILTITRPDTHGTKTSLTAALYSDTTKKATLDTIFTVVTSPDSNKAKMWG
                     HMPETVTAADGTVFKRPLLLKELSSTSGRTAIAEENEDWAQFTQTQATSTSSNGCGSE
                     YVPSQAGLESLYEANRGNAMKTVQGWPVASSYLTSTTGSSSLEQRDFKAVNLSSGTSS
                     IIPSATKELLTCQTTPIVKASQIVLEAADPTKFDRMNNVVKAKKGEEAVLRVTTKDAQ
                     GNPVGNTAFTLKRNTSVNRANVSTTTSIASLAVTDAWGNTQDDFLSTTLVIYGVTGAD
                     GITTFTLKQDQTTGLKTELTAALDSSSSTKSTLPVVFTVLTSPDSPKAKFWGHMAETA
                     TGDDGLIYRRPLLRDENSATTSIGTLVEEGEAWSTFPSGQANDTSINGCGAEYVPTDN
                     ELRAIYAHQGSSALHDAIGWPVSRFYISNTVADTFTQTFTYDVVSLKTGDETQMPSSG
                     GALLSCRTTPVAVASQIIVEANDTAQFVKVDDTLSALKVKKGEDAVIRVVTKNAQGNS
                     VPNVPFILRREGSKNRQNAEMINKSITVINAAGASARMNSSSSLLYGVTGADGTTSFT
                     VKQDDSMGLVTNMYAQLYQPTIESNKLPVMFTVITSPDTPLASYWGHMPETFTTRSGI
                     AFKRPLLTAEHPAGQSTMANNESWLSLNTAAKNDVSKSDCGEPYQPLLSEFQELYSEH
                     PNGAIGTDLGLPLTNTWWAYDKIAYANVWYDQSINLSNGSSSRALSNTVAFVSCLVNP
                     HAVAASIEMTSTALDAEKTASNDGRPSATAAKGTAIRMTVIVRDSAGNLLPGANFNLI
                     RGTALDRAKNRLDSTYDDLTIVPVTPAGVNMSLYNNGAQALLTTGSDGKATFDVTQNE
                     TYGLATPLTATLMRDTTKSATMDVIFTVITSPNSPKAKYWGHMPDTFTSRAGVTFKRP
                     LLAAEATLGSSVSNNNESWSYLFYTNKVTPDCPVEYQPRLNELQGLYNDHPGGTILTD
                     LGLPITAGSGNWWTYEMSTTDALTWYYGVINLKTGQSTTTINGYALMLCLTQPHSAPA
                     SLTLSSTAYDEGRTASNGGTPTSSVKKGEMLPIVVTIKDANGNPVGGEGVTLKRVQAK
                     SRSGISVSSNTVDDLILDEVTPTSARISFNQNTSAWSGFTGSDGTITFNVTQNNTVGL
                     VTPFTASLARNPQVTANQDLIFTVVTSPDSAKANYWGHMPATLTAVNGAVFERPKLWS
                     ELTSTSGVGKINNNNEDWPYFTPTQKSDASVSPCEVARQPLFNDLSSLSARYPNNTFV
                     TETGWPAYYTWWAEDKSADGKDQSVDLRNGTLYTGSTKSFQPCLANARSTVSSVTLTS
                     TAFDAATQAAKVKKGEAMPVTVTVKDSAGNTVPNVEFTLKRGEASPRNAGATLYGDVV
                     AMDDLIVQPLSGSAVTLSESGNTISGMTGADGTASFTLRQDNTPGYKTPLTVTLANYA
                     SATDTLDAIFTVPTSPNVSSAHFWGHMADTVVVNGKSLHRPLLTTELPSGANPVSSPI
                     INYENWASAHIIDASKWDIARQCGSIENAPTYNELELLHTVFNSLGWPSSPSFPYLSS
                     QQCGMDEGTGAQDCSITLINKPGLVTCFQ"
     gene            complement(14072..15049)
                     /locus_tag="ACHJ8C_003280"
     CDS             complement(14072..15049)
                     /locus_tag="ACHJ8C_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family autotransporter-associated protein"
                     /protein_id="HFO5553085.1"
                     /translation="MKQDKRRGLTRIALALALAGYCVAPVALAEDSAWVDSGETNIFQ
                     GTIPWLYSEGGSATTDADRVTLTSDLKGARPQGSETDKRLYSGDKLTVSWEIGDTEGD
                     VDLGGLGDNAKTIDTIRWMSYKDAQGGDPKELVTKVTSYTLTDADRGRYIGIEITPTT
                     QTGTPNVGTALHLYDVSTASGGGSDSDNVAPGPVVNQNLKVAIFVDGTSINLINGSTP
                     IELGKTYVAKLYSDENKNGKFDAGTDADVTANYDFRWVLSGSSQQLGTSGGIVNSSFD
                     NNNLVIPATNDEARTNLNGPARDGKEALSIPTNGDGVQGYKLHIIYKHK"
     gene            complement(15104..17281)
                     /gene="sinH"
                     /locus_tag="ACHJ8C_003281"
     CDS             complement(15104..17281)
                     /gene="sinH"
                     /locus_tag="ACHJ8C_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HFO5553086.1"
                     /translation="MLRWKRCIILTFISGAAFAAPEINVKQNESLPDLVSQAAQQDEQ
                     TNKGKSLKERGADYVINSATQGFENLTPEALESQARSYLQSQITSTAQSYIEDTLSPY
                     GKVRSNLSIGQGGDLDGSSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRHN
                     FDKWLLGGNIFYDYDFTRGHRRLGLGAEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEE
                     RPARGWDIRAEAWLPSYPQLGGKIVFEQYYGDEVALFGTDNLEKDPYAVTLGLNYQPV
                     PLLTVGTDYKAGTGDNSDVSINATLNYQFGVLLKDQLDSDKVKAAHSLMGSRLDFVER
                     NNFIVLEYKEKDPLYVTLWLKADVTNEHPECVIKDTPEEAVGLEKCKWTINALINHHY
                     KIVAASWQAKNNAARTLVMPVIKENTLTEGNNNHWNLVLPAWQYSSDKAEQEKLNTWR
                     VRLALEDEKGNRQNSGVVEITVQQDRKIELIVNNIANVPEENNHSHEASAQADGVDGV
                     VMDLDVTDSFGDNTDRNGDALPEDNLTPQLYDAQDKRVTLTNKPCSTDNPCVFIAKQD
                     KEKGTVTLSSTLPGTYRWKAKAAPYDDSNYVDVTFLGAEIGGLNAFIYRVGAAKPSNL
                     IGKDKEPLPLNNTYRFVLWRDNNKDGVFQQVEKLTDEEMVQYDYKWEFTGKSINGEVG
                     AQANTSNEDIVIPATNREAAQTYGAQAGDGLQGYGLRVLYTKK"
BASE COUNT         4068 a         4603 c         4308 g         4327 t
ORIGIN      
        1 taacaaggaa tagtgatgaa atttaaaaaa tgtcttctgc ctgtggcaat gttagcgtca
       61 ttcactctgg caggatgcca gtcaaatgct gatgatcatg ccgccgatgt ttatcaaacc
      121 gatcaactga ataccaaaca agaaactaaa accgttaata ttatttccat tcttcccgca
      181 aaagttgccg tagacaactc ccaaaataaa cgaaatgcac aagccttcgg cgcgcttatt
      241 ggtgcagtcg ctggcggtgt aatcggccac aacgtggggt ctggcagcaa ttccggaacg
      301 acggcaggtg cagttggcgg cggagctgtt ggcgcggcag cgggttctat ggtgaatgat
      361 aaaaccttag tggaaggtgt ttctctaacg tataaggaag gcaccaaagt gtatacctcc
      421 acccaggtgg gtaaagagtg ccagtttacg acaggtttag ccgttgttat taccacgacg
      481 tataacgaaa cgcgtattca gccaaatacg aaatgtcctg aaaagagcta atgatcagga
      541 ggagtcatga agaaagtttt tctttgcgcc atcttagcct ccttaagcta tccggctatc
      601 gcctcatcat tgcaggatca actctcggct gtcgcagaag cggaacagca aggtaaaaat
      661 gaagagcaaa ggcagcatga cgaatgggtc gcggagcgca acagggaaat ccagcaagag
      721 aagcaacgtc gcgcaaacgc ccaggccgcg gctaataaaa gagcggcaac ggcagcggcg
      781 aataagaaag ctcgtcagga taaactggac gccgaagcca ctgcggacaa aaaacgcgat
      841 caaagttatg aagatgagct acgcagctta gagattcaga aacaaaaact ggcgctggcg
      901 aaagaagaag cccgcgttaa gcgagaaaac gaatttatcg atcaggaact gaagcacaaa
      961 gctgcgcaaa ccgatgtggt gcaatctgaa gctgacgcaa acaggaatat gactgagggc
     1021 ggtcgagatc tgatgaaaag cgtgggcaaa gcagaagaga acaaatcgga cagctggttt
     1081 aattaatcga tgttagtaac ttcaatccta tgattcgtga gtataaaaaa ccctctgtag
     1141 taacagaggg ttttgttcat tcatagtgca gggatcaaaa tcattcccac tcaatggtag
     1201 ctggcggctt gccgctgatg tcatacacca cgcgggaaat accgttcact tcattgataa
     1261 tgcggttgga aacgcgaccg aggaaatcgt acggcagatg cgcccagtgt gcggtcataa
     1321 agtcgatggt ttcgacagca cgcagagaga caacccagtc atacttacga ccatcgccca
     1381 ttacgccaac ggaacgtacc ggcaggaaga cggtgaatgc ctggctgact ttgtcgtaca
     1441 ggtccgcttt acgcagttct tcaatgaaga tggcgtcagc acggcgcagc agatcacagt
     1501 actctttctt cacttcaccc agaacacgaa cgccgaggcc tggtcccggg aacgggtgac
     1561 ggtagagcat gtcgtacggc aggcccagct ccagaccaat cttacgcact tcgtctttga
     1621 acagctcttt cagcggttca accagcccca tcttcatctc tttcggcagg ccgcccacgt
     1681 tgtggtgaga tttgatgacg tgcgctttac cggttgctga tgcggcagat tcgataacat
     1741 cagggtagat agtaccctgc gccagccact tcacatcttc cagtttcagc gcttcttcat
     1801 cgaatacttc aacgaaaacg cgaccgatga ttttacgttt tgcttccgga tcgttttcgc
     1861 cagccagcgc tgacaagaag cgatcttctg ccggtacgtg aacaatattc agaccaaagt
     1921 gatcgccaaa catatccaga acctgctctg cttcgttgag acgcagcagg ccgttgtcga
     1981 cgaatacgca agtcaggttt ttaccgatag cgcggtgcaa cagcattgcg gttacggagg
     2041 aatccacacc accagagagg ccgaggatga ctttatcgtc gcctacctgc tcgcggatgc
     2101 gagctacagc atcgtcgata attttcgctg gcgtccacag ggcttcacac tggcagatat
     2161 cacgcacaaa acgctccagc atgcgcatac cctggcgggt gtgagtcact tccgggtgga
     2221 actgtacgcc atagaagcgt ttttcttcgt tagccataat ggcaaacggg cagctttcgg
     2281 tgctggctac ggtgacgaag tcggacggga tagcggtaac tttatcgccg tggctcatcc
     2341 agacgtccag cagcggttta ccgtctgcgg tcagagcatc ttcaataccg cgaaccagtg
     2401 cgctgtcgtt tacgacttca acctgcgcgt agccaaattc acgttcgtta gaggcttcaa
     2461 cgtgaccgcc caactgcatt gccatggtct gcatgccata gcaaacgccg aataccggta
     2521 cgcctgcttc aaagacatac tgcggcgcac gcgggctgtt ttcttcggtg gtgctttccg
     2581 ggccgccgga aagaataatg ccgcttggat tgaagtcacg aatttgtgct tctgtcacat
     2641 cccacgccca cagttcgcag taaacgccca gctcacgcac gcggcgcgca accagttgag
     2701 tatactggga accgaagtcc agaatgagga tgcgatgctt atgaatgttt tccgtcattg
     2761 acgcttattc cgaggcaagt gaaacagata atataaatcg cccgacatga agtcgggcga
     2821 agagaatcag gagcccagac ggtagttcgg ggactcttta gtaatggtca cgtcgtgaac
     2881 gtggctttcc tgaatgcccg caccgctgat acgtacaaac tccgctttag tacgcagttc
     2941 gtcgatagta ccacagccgg tcagccccat acaggagcgc aggccgccca tctgctggtg
     3001 aatgatctct ttcaggcgac ctttataggc cacgcgacct tcgatacctt ccggcaccag
     3061 tttgtcggca gcgttgtcgc tctggaagta acggtcagaa gaacctttgg acatcgcgcc
     3121 cagggaaccc ataccacggt aagatttgta agaacggccc tggtagagtt cgatttcacc
     3181 cggagattct tcagtacctg ccagcatgga acctaccatc accgcgcttg cgccagcggc
     3241 gatagcttta gcgatatcgc ctgagaaacg gataccgccg tcagcgataa ccggaatgcc
     3301 ggtaccttcc agggcttcta ctgcatcagc aacggcggtg atctgcggaa caccgacacc
     3361 agtcacaata cgcgtcgtac agatagagcc agggccgata ccgactttaa ccgcgctgca
     3421 accggcttct gccagagcgc gagcgcccgc agccgttgcc acgttgccgc cgatgatttg
     3481 cagatccgga tatttagcac gggtttcacg gatacgttgc agtacacctt cagagtgacc
     3541 gtgagaggag tcgatcagca gaacgtcaac gcctgcggca accagcgcat caacacgctc
     3601 ttcgttacca gcgcccgcgc caaccgctgc acctacacgc agacggcctt gttcgtcttt
     3661 acacgcgttc ggtttacgtt ccgctttctg gaagtctttc acggtgatca tgccgatcag
     3721 gtggaattcg tcatcaacca ccagcgcttt ttcaacgcgt ttttcgtgca tttttgccag
     3781 cacaacttca cgggcttcac cttcacgcac ggtgaccaga cgttctttcg gcgtcatgta
     3841 aacgctaacc ggctggctca ggtcggtaac aaaacgtacg tcacgaccgg tgataatacc
     3901 caccagttcg ttttcttcgg taacaaccgg gtagcccgca aagccgttac gctcggtcag
     3961 ttctttcact tcgcgcagcg tcgtggttgg cagtacggtc tgcggatcgg ttaccacgcc
     4021 agattcgtgt tttttcacgc ggcgaacttc ttcagcctgg cgctcaatag acatgttttt
     4081 gtggataaag ccgataccgc cttcctgggc cagggcgata gccaggcgcg cttccgttac
     4141 ggtatccatt gctgcggaaa gcatagggat attcagacga atagttttcg tcagctgggt
     4201 gctgaggtca gcagtattcg gcagaacggt agaatgagca ggaacgagga gaacgtcgtc
     4261 aaacgtcaga gcttctttag cgatacgtag catgggcaat atctcgacca gagtggttaa
     4321 taaatattgc cgcggcatta tacagagcgt aaccgattgc atctacccct ttttgcaaaa
     4381 aatgcttgct atccccggag ggcgggttac tatcgaccga ataacctgct gatttagaat
     4441 ttgatctcgc tcacatgtta ccttctcaat cccctgcaat ttttaccgtt agccgcctga
     4501 atcaaacggt tcgtctgctg cttgagcatg agatggggca ggtgtggatc agcggcgaaa
     4561 tctctaattt tacacaacca gcctccggtc actggtactt tacgcttaaa gacgataccg
     4621 cgcaggtgcg ctgtgcgatg ttccgaaaca gcaaccgccg ggtcaccttc cgcccacagc
     4681 atgggcaaca ggttttagtt cgcgccaata ttacactcta cgagccgcgc ggcgattatc
     4741 aaattatcgt cgaaagtatg cagcccgccg gtgaaggttt actgcaacag aagtacgaac
     4801 agcttaaagc gaagttgcag gctgaaggtt tgttcgatca gcaacttaaa aaaccactcc
     4861 cctcccctgc gcactgcgtt ggggtgatta cctcaaaaac aggtgccgca ctgcacgata
     4921 ttttgcatgt gttgaaacgc cgcgatccgt ctctgccagt gataatctat cccactgccg
     4981 ttcagggcga tgacgcacca gggcaaatcg ttcgcgccat tgagctggcg aatcttcgca
     5041 acgagtgcga cgtattaatc gtcgggcgtg gcggtggctc gctggaagat ttatggagtt
     5101 ttaatgatga gcgtgtggcg cgggcgattt ttgccagtcg cattccgata gtcagcgccg
     5161 tcgggcatga aaccgatgtg acaattgctg actttgttgc tgatctacgt gcgccaacgc
     5221 cgtcagccgc cgctgaagtg gtgagccgaa atcagcaaga attgctacgt caggtgcaat
     5281 cgacccatca acggctggaa atggcgatgg attattatct cgccaaccgc acgcgtcgtt
     5341 ttacgcagat tcatcatcgc ttgcagcaac agcatccgca gctccggctg gcacgtcagc
     5401 aaactatgct cgaacgtctg caaaagcgga tgagctttgc gctggagaat cagcttaaac
     5461 gcgcgggtca gcaacagctg cgattaacac ggcaactcgt ccagcaaaat ccgcagtcgc
     5521 gtattcatcg cgcgcaaacg cgcattcagc aactggaata tcgtttagca gaaaccctgc
     5581 gcgcacagct tagcgccacg cgtgaacgtt tcggtaatgc agtaacacac ctcgaagccg
     5641 taagcccact gtcaacgctg gcgcgcggtt atagcgtcac cagcgccgct gatggcgcgg
     5701 tgttaaaaca ggttaagcaa gtgaaagtgg gtgagacact gaccacacga ctgggagatg
     5761 gtgtggtgat tagtgaagtc agcgccgtaa caaaaacccg caaatcacgt aaaaaaacgt
     5821 ctaatccgta gaccggataa gaggcgccag catcgcatcc ggcaataacc cccaggccac
     5881 gccagatccg ccatgctggt tactggaagc aggtcaccag acccggttta tttattagcg
     5941 tgatactaca atcctgtgct ccggtacctt cgtccatccc acattgttga gaggacaaat
     6001 aaggaaaact gggtgaagac ggccatccca gtgaattaaa aacagtatgc aacaactcta
     6061 attcattgta tgtcggtgca ttttctatgc tgccacactg ccttgcgata tcccatttac
     6121 tggcatcaat gatatgtgca gaagcccagt tttcgtagtt aataatcggt gaggatacgg
     6181 gattagcccc ggacggcaat tctgtcgtta acaacggtcg atgtagcgat ttaccattca
     6241 ccactaccgt gtctgccata tgtccccaaa agtgcgccga ggaaacattt gggctggttg
     6301 gtaccgtaaa aatagcgtcc agcgtgtctg tcgcggaagc gtaattagcc agagtaaccg
     6361 tcagtggcgt tttataacca ggggtattat cctggcggag cgtaaaactt gccgtcccat
     6421 ccgcgcctgt catgccacta atggtattac cgctttcact cagcgtaaca gccgaaccag
     6481 ataacggctg aacgatgaga tcgtccatcg ccaccacatc accatacaac gttgccccag
     6541 cgttacgcgg cgatgcctca ccgcgtttca aagtaaattc cacattcggc accgtattcc
     6601 cggcgctatc tttcaccgtc acggtaacgg gcatggcttc gccttttttc actttcgccg
     6661 cctgagtggc tgcatcaaac gctgttgagg taagtgtgac gctggaaacc gttgaacgcg
     6721 cgttagccag gcaaggctga aatgattttg ttgaacccgt atacagcgtg ccatttctca
     6781 aatcaaccga ctggtcttta ccatcggcgg atttatcctc tgcccaccaa gtgtaatacg
     6841 caggccagcc agtttcagtg acaaacgtgt tattcggata acgcgcagac aacgaactta
     6901 aatcattgaa tagtggctgc cttgctactt cacaagggct cacactcgca tctgatttct
     6961 gtgttggggt aaagtacggc cagtcttcat tattgttatt gatcttgccg acgccagaag
     7021 tcgatgtcag ttcactccac aatttcgggc gttcaaatac cgcaccattc actgccgtca
     7081 aggttgctgg catatgcccc cagtagttag ccttcgcaga atccgggctg gtcactaccg
     7141 tgaatatcag gtcctgatta gccgtaacct gcggatttcg cgccaatgaa gctgtaaacg
     7201 gcgttaccag accgacggtg ttattttggg taacgttgaa cgttatcgtg ccatcactgc
     7261 cggtgaagcc gctccatgcc gacgtattct ggttgaagga gatacgcgcc gatgtcgggg
     7321 tgacttcatc taaaatgaga tcatctacgg tattggagga tacagagatc cctgaacggc
     7381 ttttcgcctg cacgcgcttg agcgtaacac cttcgccgcc aacaggatta ccattggcat
     7441 ctttgatcgt caccacgatg ggaagcatct cacctttttt cacagatgac gtgggggtac
     7501 caccattgct ggcagttcgg ccctcatcat aggcagtcga tgacagcgta aggcttgctg
     7561 gtgccgaatg tggctgcgtc agacagagca ttaaagcgta tccattaata gtcgtcgtcg
     7621 attgtcccgt tttcaaatta atgacgccgt aataccacgt caacgcgtca gtggtgctca
     7681 tttcataagt ccaccaattt ccacttccgg cagttatagg caaccccaaa tctgtcagta
     7741 ttgtcccgcc tggatgatca ttatatagcc cctgtaactc attgagacgc ggctgatact
     7801 caaccgggca atccggcgta actttattgg tataaaaaag gtatgaccac gactcattat
     7861 tattacttac cgaggacccc aaggtcgctt ctgctgccag tagcggtcgt ttaaaggtca
     7921 cccccgccct actggtaaac gtgtcgggca tatgacccca gtattttgct ttcggtgagt
     7981 tcggactggt tatcacagtg aaaataacat ccatcgtggc actttttgtg gtgtcgcgca
     8041 tcaatgttgc agtgagcgga gttgccagcc cataagtctc gttttgcgtc acatcaaaag
     8101 tcgctttacc atcacttcct gtcgtcagaa gtgcctgtgc cccattatta tagagtgaca
     8161 tgtttactcc ggcaggggtg accggcacga tagtaagatc atcatacgta gaatctagcc
     8221 tgttttttgc acgatccaac gcagtcccgc gtatgagatt aaagtttgcg cctggcaaca
     8281 gatttcctgc gctatcccgc acaatcaccg tcatccggat cgccgtacct ttcgccgccg
     8341 ttgcactagg gcgaccatcg ttgctggccg ttttctccgc atccagtgcc gtggaggtca
     8401 tctctataga agctgccact gcatgagggt ttaccagaca ggacacaaat gccacggtgt
     8461 ttgataatgc cctggagctt gatccattcg ataaattaat agactgatcg taccagacgt
     8521 tcgcgtaggc aattttatcg taagcccacc aggtgttagt cagcggaagg cctaaatcgg
     8581 tccctatcgc accattagga tgttcactgt ataactcttg aaattcgctt aataacggtt
     8641 gataaggttc gccgcagtct gatttgctga catcattttt tgctgccgta ttcaaagaaa
     8701 gccatgattc attattagcc atggttgact gccccgctgg atgctcggca gtaagcaatg
     8761 gccgcttaaa ggcaattccc gaccgggtag taaacgtttc aggcatatgc ccccagtaac
     8821 ttgccagtgg cgtatccggg ctggttatga cggtaaacat gaccggtaat ttgttggatt
     8881 ctatcgtcgg ctgatacaac tgggcgtaca tattggttac caggcccata gaatcatctt
     8941 gttttaccgt aaaggaggta gtcccatccg cgccagtcac cccataaagc aggcttgaag
     9001 aactattcat cctcgcagaa gcgcctgccg cgttaattac cgtaatcgac ttatttatca
     9061 tctcggcatt ttgacggttc ttcgagcctt cacgtctgag aataaagggg acattgggca
     9121 cgctatttcc ctgcgcattt ttagtcacca cgcgaatcac cgcgtcttcg ccttttttca
     9181 ctttcaacgc actcaaggtg tcgtcaactt tgacaaattg cgccgtatca ttcgcctcaa
     9241 cgatgatctg actggcaacg gcgacaggcg tggtccggca gctcagtaat gcaccaccag
     9301 atgagggcat ctgcgtttca tctcctgtct tcagcgaaac gacatcatac gtaaaggttt
     9361 gagtgaacgt atctgcaacg gtattcgaga tatagaacct cgacactggc cagccaatag
     9421 catcatgtaa tgcgctactc ccctggtgcg cataaatggc cctaagttca ttgtcagttg
     9481 gaacatactc cgcgccacaa ccatttatcg aggtatcgtt agcctggccc gacgggaacg
     9541 tagaccaggc ctccccctct tccaccaggg taccgatact ggtggtagca ctattctcgt
     9601 ccctcagtaa aggccttcga taaataagac cgtcatcgcc tgtcgccgtt tcagccatat
     9661 gcccccagaa tttggctttt ggcgaatccg ggctggtaag caccgtaaag acaacgggaa
     9721 gcgtcgattt agtgctgctg gaggaatcca acgctgccgt gagttctgtt tttagcccgg
     9781 tagtctggtc ttgcttcaat gtgaaagtgg tgatgccatc agcccccgtc acgccataaa
     9841 taaccagggt cgttgaaaga aaatcatctt gagtatttcc ccaggcatcc gttaccgcca
     9901 gcgaagcaat cgacgtcgta gttgacacat tggccctgtt cacgctggtg tttcgtttga
     9961 gggtaaatgc ggtatttccc acagggttac cctgcgcatc tttagtggtc actcgtaata
    10021 cggcttcttc gccttttttc gctttgacaa cattattcat acggtcaaat tttgtggggt
    10081 ctgcagcttc aagaacaatt tggcttgcct taacaatcgg cgtcgtctgg caggtaagca
    10141 gttcttttgt ggccgatgga attatcgaac tggtgcctga actgagattg acggccttga
    10201 agtcacgctg ttccagacta ctgctgcctg tcgtgctggt aaggtaactt gaggcgacgg
    10261 gccagccctg aaccgttttc atcgcattgc cgcggttggc ttcatacaat gactccagcc
    10321 cggcctgaga gggaacatat tcactaccac aaccattaga ggatgtgctt gtcgcctgag
    10381 tttgggtaaa ttgcgcccag tcttcattct cttctgcaat cgctgttcga cccgacgtac
    10441 tggataactc tttcaacaat aatgggcgtt taaagaccgt tccatccgca gccgtcaccg
    10501 tttccggcat atgcccccac attttcgctt tattgctgtc cgggctggtg acaacggtaa
    10561 aaatggtatc gagggtcgct tttttcgttg tgtctgaata gagcgcggcg gtcagcgacg
    10621 tttttgtgcc atgggtatcc gggcgggtaa tagtgagaat tttagtgcca tcgctgccgg
    10681 tcatcgcgct gtatgctgcg gcagtatcat tgagcaaagt ttcatccgcc aggccactat
    10741 tgatcacaac cggcgcaacc agcgcatcac cgctaccggc aatgtgtttt tcatttgatc
    10801 gggtataacc atcgccacgc ttcagggtaa aaggcatatc acccaatgga ttgccctgag
    10861 catcttttac cgtcaccttc acctgtagca tttcgccttt tttcagcttc gccgcatttg
    10921 cagccgcgtt ccactgcgcc ggatctacca cttccagcgt aatgctgctg gcaggattgt
    10981 tagccgtcgt caggcaactg acataaaccg ccgtgctacc gccaacggtc cgcgccccat
    11041 cgttgagggc tatggtgtaa taatgcggta cctgatccgc gggcgaactg ctccagtagg
    11101 gctgacgctg cgttggccag ccgtgtactg tctgtacacc gttaccgtta ttggcatcat
    11161 agagcgcgct aagctgagaa cggcgtggga gcatattggc gccacagcca cctttactgg
    11221 tgctggaggc gttggcctgg ctgactcgcg accagacttc gttattttcc gttaatgtgc
    11281 cgtccggact gaacacttcc gcagccagtc ggggtcgggc aaaggttaaa gcatcgacgg
    11341 taatcgtctc atccatatgc ccccacatct gcgcgccaac cgcatccgga ctggtgagcg
    11401 tagtgaaaat cacattgtaa ttaacagtat tgggaatata ggagttcaca ggcacaatat
    11461 ttagcggcgt cgataagccc acaccttgcg attgcttaat ttccacagtc gcaaaaccct
    11521 gcgcatccgt agtaccggca tagactaaag acggttgcga tgttccgtag agtgtgccat
    11581 tcatctcgat tgcgccgcgg gttggatcat caaaacctgt agtctgcccc tgacgatttt
    11641 tccccatatc tttagtgatg gtgaacgcgg tatacggcac gggtgcatta ttcaaggcat
    11701 taaaggtgcg taccgtcatg gtgatcgtgt cacccacttt ggcctgcgct ctgctgcctg
    11761 cggaaacatc acgatcggtg gtcgcggcaa tctgggcgac catctcattg cccgaacaag
    11821 agagatagtt ttgcttaaag ccggaatagg tgtcgatgtt gcccgtggcc aggtttaccg
    11881 acgcgtgcga cgtctcttcc accgcactaa gatacgcttt ttgcaacgtt ggccagccat
    11941 attcggtgcc gatggaattg gctggatgag cggcaaacaa actgtgcaat gaactttggc
    12001 tcggaatatg acctaaacca cactcagcct gcatactgct attctgatca aacagcgccc
    12061 agtcttcgtt attttcccgc accgatccca gttcgttgtc ggtttcatcc gccagccgtg
    12121 ggcgtttaaa gatattgttc gcttcaataa tacccaacat atggccccac atccgcgctt
    12181 tgtcggtatc ggggctggtg atcaccgtaa aaataacgtc tttctcatcc gaggcagtaa
    12241 aatcactgcg cattctcgcg gtgatgtgag ttttcacccc cacgccgcct ggctgggtca
    12301 gcgtcagagt ggcctctccg ttagcatcag tgattccttc ataggtatgg gcgtcaactt
    12361 tgcgaacgtt actgccgcca tcaatttgta ccggatccgt ctcccacgcg gcgtcagttt
    12421 gattcttacg gttaatgccg tcatccagat gcagcgaaaa gtagtagtaa gccagcggct
    12481 gatcgttgtt cttgctatcg gtaatcgtca gacgtaaaga agcctcttcc cccaccttta
    12541 ctttcatcgc ttttatcgtc gaatcatagc tgcccggcgt cagtaccagc ttcggttcaa
    12601 catcgggcgc ttctttatcg acacagctca caataaacgt cgtatcgtcc ggtttttgcg
    12661 cttcgctgcg gttcgaaaca tccaccgcat ggtgctggcc gctcatcccc gccagtgatg
    12721 accagtagaa attaccctgc atcggccaac cagccacatc ctgcactgac gttggcacca
    12781 ccgtcgccag cgcaccgaac tgacgcattc cgggcaaaat gtcgcaatgt ttatccgcgc
    12841 cgctccaggt aaaggtggac caggtttcat tgtgatcgtt aaccgtaccg tcttcattat
    12901 cgacttccgc cgccagtttt ggtcgactaa aagtgtaatc acgggccttc agcgtatcgg
    12961 gcatatgacc ccacattgta gcctgcggga catccgggct ggtgaggacg gtaaagatca
    13021 ctgccgtttc actggcctga gcaatacccg cgaggctcgc gaccaacggc gtttttacac
    13081 cagggccgtt ggcctgggtg accgtaaccg tggcaacacc attcgcatcg gtagtaccac
    13141 gatattccgt cgcggtggtg gttaattcgg tcgtgcccag ttttacaggc gcagtattgt
    13201 tcaccacacc ctggcggttt tcagcatctt tgcgtttgat aacaaaagga atattcccca
    13261 caacattacc gacgcaatct ttggtggtga ccgttaacgt gatactgccg ccaaccgtca
    13321 ttttcgccgc acctgaggat tcgtcatagc ccgcagcgct gacggaaata aggtcaccct
    13381 gcgcgggcgc aatcttcagc gtcactttct gatcggcgag cgtcagaaca ctttcatagg
    13441 gatagcccgt cgaggtgagt aaatataaca gcggcatggt ctgctgggga tcaggctgcg
    13501 cccaggcgac cagcgaacgc ccggcaagtc cctgtgcaca aaacgagcga tctgcgactg
    13561 gctgtgggtc cagtaaggtg tccggcgtgg cggcatccgc ccaaactaaa gatacagccg
    13621 gtggcgtttg gttttcccaa cgtggggtgg caatgatatc gccttctctg tcttgcgggt
    13681 tggtgacgag caatttagag acgctaacat ccgcgttgaa ttcactcggt ttagcggtaa
    13741 acgctacctc atgcgtcttt gtaggatcca gaaagacact gccctggtag acgggtatgc
    13801 tggcgctgtc ggcggtgggc acggtaccgt taaattcgcc gaccgtatct gaagagtctt
    13861 gccatgcaga ttctgcccat gaccacccag gcagcattgc gcttatcagc aatgcgcata
    13921 acacaaggat tttattcagg gtcagggaca tgacagaaac tccaccggtt gttaaacgga
    13981 cgcttgtcgc atcaacatat tttgcttaat ccgggattaa gcaaaatatg cggaaacagc
    14041 attcccttgc gggaatgctg ctgagtacgc gttatttgtg tttgtaaata atgtgaagtt
    14101 tgtaaccctg taccccgtcg ccgttggtcg ggatggaaag tgcctctttt ccatcgcgcg
    14161 cagggccgtt aaggttggtt ctggcttcgt cgttggtcgc agggatgacc aaattgttat
    14221 tatcgaagct tgagttaacg atgccacccg aagtgccaag ctgttggctg ctgccagaaa
    14281 gtacccaacg gaagtcataa ttggcggtga cgtcagcatc ggtacccgca tcaaacttgc
    14341 catttttgtt ctcatccgag tacagtttgg ccacgtaggt tttgccaagt tcgattggcg
    14401 tgctaccgtt gataaggttg atactggtac catcaacaaa gatggcgact ttcaggttct
    14461 ggttaaccac cggccccggt gcaacgttat cgctgtcgct tccgccgccg ctggcagtag
    14521 aaacgtcata aagatgcagc gcagtcccga cgtttggcgt accggtctgc gtggttggcg
    14581 taatttcaat accgatataa cgaccacggt cggcatcggt aagagtgtaa ctggtcacct
    14641 tcgttaccag ctcttttgga tcacccccct gcgcatcctt gtagctcatc cagcggatag
    14701 tatcgatagt tttcgcgtta tcgcccaatc ccccaagatc cacgtcgcct tcggtatcac
    14761 caatttccca acttacggtt aatttatcac ccgagtaaag acgcttgtcc gtttcactgc
    14821 cttgcgggcg agcgcctttt agatcagaag tcaacgttac acggtcggca tctgtcgtag
    14881 cacttccccc ttccgaatag agccacggaa tggtcccctg gaaaatattg gtttcaccgc
    14941 tgtcgaccca ggcgctgtct tcagccagcg ccacaggtgc cacacaataa cctgccagtg
    15001 ccagcgctaa tgcgatccgg gtcagaccgc gtcttttatc ctgcttcatt gatgctctcc
    15061 ttaatgaacc ttcagcgact ggccgactgg ccttacgcca atcttatttt ttggtataca
    15121 gcacgcgtaa accgtatccc tgcaagccat ctcccgcttg tgcgccatag gtttgcgccg
    15181 cttcacggtt agtggccgga atcacaatgt cctcattgga agtgttcgcc tgtgcaccca
    15241 cttcaccatt gatgctcttg ccggtaaatt cccacttgta gtcgtactgc accatctctt
    15301 cgtcagtcag tttttcgacc tgctggaaga caccgtcttt attgttgtcg cgccacaaaa
    15361 caaatcgata agtgttgttg agcggcaacg gttctttatc tttacctatc aggttgctgg
    15421 gtttagccgc ccccacacga tagataaaag catttagccc accaatttct gccccgagga
    15481 aagtgacatc cacatagtta ctgtcatcgt agggcgcggc ttttgctttc cagcgatagg
    15541 tgccgggtaa ggtactggag agggtgacag tgcccttttc tttatcttgt ttggcaataa
    15601 aaacgcaggg gttatcggtc gagcagggct tgttggttaa cgtcactcgt ttgtcctgcg
    15661 cgtcgtaaag ctgaggcgta aggttatctt ccggcaacgc atcaccgttg cggtcggtgt
    15721 tatcgccaaa gctgtcggtt acatcgagat ccatcactac accatcaacg ccatctgcct
    15781 gagcgctggc ttcgtggctg tggttgttct cttctggcac gttcgcgatg ttattaacaa
    15841 tcaactctat tttacggtcc tgctgaacgg tgatttccac cacgccagag ttctgtcggt
    15901 tgcccttttc atcttccagc gccagacgta ctcgccaggt attgagtttt tcttgttcgg
    15961 ctttgtcaga gctgtactgc caggcgggca gcaccaggtt ccagtggtta ttgttaccct
    16021 ctgtcagggt attctctttg ataaccggca tcaccagcgt gcgggcagca ttgtttttcg
    16081 cctgccagga ggcagcaacg attttgtaat gatgattaat gagtgcgtta atggtccact
    16141 tacatttttc cagaccgacg gcttcttcgg gcgtatcttt aatcacgcac tcgggatgtt
    16201 cattcgtgac gtcggctttc agccacaaag tgacatagag gggatctttt tctttgtatt
    16261 ccagaacaat aaagttatta cgctcaacga aatcaagacg gctgcccatc agcgagtgcg
    16321 ccgctttcac tttatcgcta tccaattgat cttttagcag aacgccgaac tgataattaa
    16381 gagtggcatt aatgctgaca tcactgttat ctccggtccc cgctttatag tccgtcccaa
    16441 ctgtcagtaa cggcactggt tgataattca gtccaagcgt taccgcgtag ggatctttct
    16501 ccaaattatc cgtaccaaaa agggcgactt catcgccgta atattgctcg aagacaattt
    16561 tgccccccag ttgcggataa gaaggtaacc aggcttcggc acgaatatcc caaccgcgcg
    16621 cagggcgttc ttcataaaag tcgaaatctt cagagtcttt ccagtcagaa agtgggtgat
    16681 aatagttgcc tgagaatttt aaataatccg tccaggcttc ggcgcctaaa cctaaacggc
    16741 gatgtccacg ggtaaagtca taatcataaa atatatttcc acccaataac catttatcaa
    16801 aattatgtct tacaccaagc cctatatttc cgatcgtacg atcttctttt cgttgcgcag
    16861 aaaattggct gaaataaaca gtggtttgat tatcgtacca gggaacaaaa taatcgatgg
    16921 aactgccatc cagatcgccg ccctgaccaa tggagaggtt cgaacggacc ttaccgtagg
    16981 gagagagtgt gtcttcaata taagattgtg cggttgaggt gatttgactt tgcagatagc
    17041 ttctggcctg agattccagc gcctcagggg tcaagttttc aaacccttgc gtggcggagt
    17101 tgatgacgta atcggctccg cgctctttca gcgatttacc cttgttggtt tgttcatcct
    17161 gttgtgctgc ctggctaact aaatcaggta acgattcgtt ttgcttaaca tttatctctg
    17221 gcgccgcgaa agcagcacca gagataaatg ttagaataat acagcgtttc catcgcagca
    17281 tgcactttcc ctgtgacaat gataca
//
LOCUS       DAZFJL010000100        17136 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFJL010000100 DAZFJL010000000
VERSION     DAZFJL010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.100"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..1226
                     /locus_tag="ACHJ8C_003282"
     CDS             <1..1226
                     /locus_tag="ACHJ8C_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO5553087.1"
                     /translation="IPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAF
                     LSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNI
                     LDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKI
                     LVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIE
                     VSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCV
                     MLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNS
                     AQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTHNISLVMTEADKALR
                     EAKSEGGNKVIIHHI"
     gene            1407..2897
                     /gene="dgcJ"
                     /locus_tag="ACHJ8C_003283"
     CDS             1407..2897
                     /gene="dgcJ"
                     /locus_tag="ACHJ8C_003283"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO5553088.1"
                     /translation="MKLHRRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVPPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2940..3443
                     /gene="yeaK"
                     /locus_tag="ACHJ8C_003284"
     CDS             2940..3443
                     /gene="yeaK"
                     /locus_tag="ACHJ8C_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO5553089.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3444..3548)
                     /gene="yoaI"
                     /locus_tag="ACHJ8C_003285"
     CDS             complement(3444..3548)
                     /gene="yoaI"
                     /locus_tag="ACHJ8C_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO5553090.1"
                     /translation="MNDQMFVETLIITSSFFAIAAVLVLSVLLIERTG"
     gene            3718..4164
                     /gene="yeaL"
                     /locus_tag="ACHJ8C_003286"
     CDS             3718..4164
                     /gene="yeaL"
                     /locus_tag="ACHJ8C_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO5553091.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4121..4942)
                     /gene="nimR"
                     /locus_tag="ACHJ8C_003287"
     CDS             complement(4121..4942)
                     /gene="nimR"
                     /locus_tag="ACHJ8C_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO5553092.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGSLGQWARFFAMSERNLARLIVKETGVSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            5039..6220
                     /gene="nimT"
                     /locus_tag="ACHJ8C_003288"
     CDS             5039..6220
                     /gene="nimT"
                     /locus_tag="ACHJ8C_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO5553093.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSTMVVPLA
                     LNGFGWQGALLMLMSFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSIGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6275..6622
                     /gene="yeaO"
                     /locus_tag="ACHJ8C_003289"
     CDS             6275..6622
                     /gene="yeaO"
                     /locus_tag="ACHJ8C_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO5553094.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6644..6898)
                     /gene="yoaF"
                     /locus_tag="ACHJ8C_003290"
     CDS             complement(6644..6898)
                     /gene="yoaF"
                     /locus_tag="ACHJ8C_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO5553095.1"
                     /translation="MKIISFVLPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7081..8106
                     /gene="dgcP"
                     /locus_tag="ACHJ8C_003291"
     CDS             7081..8106
                     /gene="dgcP"
                     /locus_tag="ACHJ8C_003291"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO5553096.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            8139..8237
                     /gene="yoaK"
                     /locus_tag="ACHJ8C_003292"
     CDS             8139..8237
                     /gene="yoaK"
                     /locus_tag="ACHJ8C_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO5553097.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8240..8314
                     /gene="yoaJ"
                     /locus_tag="ACHJ8C_003293"
     CDS             8240..8314
                     /gene="yoaJ"
                     /locus_tag="ACHJ8C_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO5553098.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8373..8621)
                     /gene="yeaQ"
                     /locus_tag="ACHJ8C_003294"
     CDS             complement(8373..8621)
                     /gene="yeaQ"
                     /locus_tag="ACHJ8C_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO5553099.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8769..8951)
                     /gene="yoaG"
                     /locus_tag="ACHJ8C_003295"
     CDS             complement(8769..8951)
                     /gene="yoaG"
                     /locus_tag="ACHJ8C_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO5553100.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(8955..9314)
                     /gene="yeaR"
                     /locus_tag="ACHJ8C_003296"
     CDS             complement(8955..9314)
                     /gene="yeaR"
                     /locus_tag="ACHJ8C_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO5553101.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYIGYADEHSTEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9487..10125)
                     /gene="leuE"
                     /locus_tag="ACHJ8C_003297"
     CDS             complement(9487..10125)
                     /gene="leuE"
                     /locus_tag="ACHJ8C_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO5553102.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLFYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10252..10419)
                     /locus_tag="ACHJ8C_003298"
                     /pseudo
     CDS             complement(10252..10419)
                     /locus_tag="ACHJ8C_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            10420..10575
                     /locus_tag="ACHJ8C_003299"
                     /pseudo
     CDS             10420..10575
                     /locus_tag="ACHJ8C_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
     gene            complement(10668..11783)
                     /gene="rnd"
                     /locus_tag="ACHJ8C_003300"
     CDS             complement(10668..11783)
                     /gene="rnd"
                     /locus_tag="ACHJ8C_003300"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO5553103.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQALPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(11865..13550)
                     /gene="fadD"
                     /locus_tag="ACHJ8C_003301"
     CDS             complement(11865..13550)
                     /gene="fadD"
                     /locus_tag="ACHJ8C_003301"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO5553104.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(13755..14336)
                     /gene="yeaY"
                     /locus_tag="ACHJ8C_003302"
     CDS             complement(13755..14336)
                     /gene="yeaY"
                     /locus_tag="ACHJ8C_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO5553105.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSSTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(14376..15071)
                     /gene="tsaB"
                     /locus_tag="ACHJ8C_003303"
     CDS             complement(14376..15071)
                     /gene="tsaB"
                     /locus_tag="ACHJ8C_003303"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO5553106.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLALRDGDVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(15129..17039)
                     /gene="yoaA"
                     /locus_tag="ACHJ8C_003304"
     CDS             complement(15129..17039)
                     /gene="yoaA"
                     /locus_tag="ACHJ8C_003304"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO5553107.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTIDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
BASE COUNT         4220 a         4387 c         4078 g         4451 t
ORIGIN      
        1 tcatcccaca gatgaattac ctgatggttg ttgtggcttt gtttttcctt aacgccgtca
       61 ttttcctttt catgttaatg aagtatttca ctaacaaaca aattttacca acactcattt
      121 taagccttgc atttttaagt ggccttatct atttagttga aaccattgta attatccata
      181 aaccaatcaa cggcagtaca ctgatccaga caaagtcgaa tgatgtttct attttctata
      241 ttttccgcca actcagtttc atttgtttaa cctcgctggc gctcttttgt tatgggaaag
      301 acaacatcct tgacaacaat aagaaaaaaa ctggcattct gttgctggcg ctgattcctt
      361 ttttagtttt tccccttctg gcacacaatc tgagcagtta taacgctgac tattctttgt
      421 atgtcgtcga ttactgtccg gacaaccata ctgcgacctg gggaatcaac tatacaaaaa
      481 tattggtttg tttgtgggca tttttactgt tctttattat catgcgtaca cgattagcca
      541 gcgaactatg gccgttaata gcattattat gtctggcatc gctatgctgc aacttacttc
      601 tactgactct ggatgagtat aattacacca tctggtatat cagtcgcggg attgaagttt
      661 ccagtaaact gtttgttgtg tcttttctga tttataacat ttttcaggaa ctgcaactct
      721 ccagcaaact ggcagttcat gatgtactga ccaatattta taatcggcgc tactttttca
      781 acagcgtaga gtcattattg tcgcgacctg ttgttaagga cttttgtgtc atgctggttg
      841 atattaatca gttcaaacgc atcaatgccc aatggggaca tcgtgtgggt gataaagtgc
      901 tggtatcaat tgtcgatatt atccagcaaa gcatccgccc cgatgatatt ttagcgcgac
      961 tggagggtga ggtgtttggc ttgctattta ccgaactcaa tagtgcccag gcaaaaatca
     1021 ttgcggaacg tatgcgtaaa aatgtcgaac tcctgaccgg ctttagtaac agatatgatg
     1081 ttcctgaaca aatgaccatc agtattggca cggttttttc aacgggtgac acgcataata
     1141 tctcgcttgt catgacggaa gcagataaag ccttacgcga agcgaaaagc gaggggggca
     1201 acaaagtgat tattcatcat atttaagtgc aaaaatattc acagccatgc tttttgcgtg
     1261 gcttttgcat acaatttatt actattaccc ctaaatttcc cctccccagc ggcgcagaga
     1321 tgagtataat tagcgcccct gtgccaggcc gcaatcgaac tttatctggt tttctcgttt
     1381 cactaaccga aggagtgcca tttatcatga aattgcaccg tagaatgctc cggcatttta
     1441 tcgccgcaag tgtcattgtg ctgacatctt ccttccttat ttttgaactt gtcgccagcg
     1501 acagagcaat gagtgcctac ctgcgctata tcgtgcagag agcagactct tcctttcttt
     1561 atgataagta tcagaatcag agtattgccg cgcatgtgat gcgcgctctc gctgctgagc
     1621 agtcggaagt gccgccagaa cagcggcgcg ccatctgcga ggcttttgag tctgccaata
     1681 atacccatgg cttaaacctg actgcccata aatacccggg cttacgcggc acattacaaa
     1741 ccgcatccac tgactgcgac acaattgtgg aagctgcagc actattaccc gcttttgatc
     1801 aggcagtgga aggcaaccgc caccaggatg attacggttc aggtcttggg atggccgaag
     1861 agaaatttca ctattatctc gatctcaatg accgctatgt ctatttttat gagccggtta
     1921 atgttgaata ctttgcgatg aataactggt ccttcctgca gtcaggaagt attggtatcg
     1981 atcgcaaaga tattgaaaaa gtatttaccg ggcgtaccgt attatcgagc atttaccagg
     2041 atcagcgtac taaacagaac gtgatgagtt tgctgacgcc ggtatatgtc gcagggcagc
     2101 taaaagggat tgtgctgctg gatattaaca aaaacaatct gcggaatatc ttttacactc
     2161 atgaccgccc tctcctctgg cgttttctca atgtcacgct aaccgataca gattcggggc
     2221 gcgacattat catcaaccag agcgaagata atctgttcca gtatgtcagt tacgtccatg
     2281 acttaccggg cggcattcgt gtctcgttat ccattgatat tctttacttt atcacgtctt
     2341 catggaaaag cgttctgttc tggattttga cggcgttaat tttgctgaat atggtgcgga
     2401 tgcacttccg tttataccaa aatgtgtcgc gagaaaatat tagtgatgcg atgactggac
     2461 tgtataatcg caaaatttta acccctgaac tggaacagcg gttgcagaaa ctggtgcaat
     2521 ccggttcttc ggtgatgttt attgctattg acatggacaa gttaaagcaa ataaatgaca
     2581 ccctcggtca tcaggagggg gatttagcga ttacgttatt agctcaggcg attaaacagt
     2641 cgattcgtaa aagtgattat gccatccgac tcggtggcga tgagttctgc atcattcttg
     2701 tcgattcgac gccgcagatt gcagcacaac tgcctgaacg tatcgaaaaa cgtctgcaac
     2761 atatcgcgcc gcagaaagag atcggcttct cttccggtat ttacgcgatg aaagaaaacg
     2821 atacgttaca tgatgcgtat aaagcttccg atgagcgttt atatgtcaat aagcagaaca
     2881 aaaacagccg ttcatgataa ccttctgtag ttgtttgctt gtaatctcag gagcatgaaa
     2941 tgactgaaat ggctaaagga agcgtgacgc atcagcggtt aatcgcttta ttatcacaag
     3001 aaggtgctaa cttccgcgtc gttacccatg aagcggtagg taaatgtgaa gcggtgtcag
     3061 aaattcgtgg caccgcttta ggccaggggg ctaaggcact ggtatgtaaa gtcaaaggca
     3121 atggcgtaaa tcaacatgtt ctggcgatcc tcgctgccga tcaacaggcc gatctgagcc
     3181 aactcgccag ccatataggc ggattacgcg cttcgttggc cagcccggcg gaagtcgatg
     3241 aactcacagg ctgtgtcttc ggcgcgatcc cccctttcag cttccatcca aaactcaaac
     3301 tggttgccga ccccctactc ttcgaacgat ttgatgaaat cgccttcaat gcaggcatgc
     3361 tggataaatc cgttattttg aaaaccgcgg attatttgcg tattgcgcaa ccagaactgg
     3421 tcaatttccg ccgcactgcg taactagcct gtccgttcaa taagcagaac ggacaaaacc
     3481 agcacagcag caatggcaaa aaacgatgac gtgataatca gtgtctcgac gaacatttga
     3541 tcgttcatag tatgcccccg ggagatagct gttttcacgt tattgttaac gtacacaaat
     3601 ggcagtttga tgacagttcg tctgattttt tatgcaaaaa acgtaaagat tttctacttc
     3661 cttcctgcag caagcgtaaa gtaagcaggc ttattatttt ttggcaagga aaccacgatg
     3721 tttgatgtca ctttgctgat cctgctcggg ttagctgcgc tgggctttat cagtcataac
     3781 accactgtcg ccgtttcaat tctggtgttg atcattgtcc gcgtcacacc gttaagcacc
     3841 tttttcccgt ggattgaaaa acaggggcta agtatcggga taatcattct gacgattggc
     3901 gttatggctc ccatcgccag cgggacgcta ccgccctcaa ccctgattca ctcgtttctg
     3961 aactggaaat cgctggtggc aattgcagtc ggcgttattg tctcctggct gggggggcgc
     4021 ggcgtgacgt taatgggcag ccagccgcaa ctggtcgccg ggctgctggt gggcactgtt
     4081 ttaggcgtgg cgctgtttcg cggcgtaccg gttggcccgc ttattgccgc gggtctggtt
     4141 tcgctgattg tggggaaaca gtagttaatc ccgcaatata tcggcctggc gtttgcccca
     4201 ggcctttctt aaacatagta ataaaggccg ttgttgaatc ataccccagc gtatgagcaa
     4261 ctttctgtac agtatcgcct ttcactaatc cctgaagcgc cataatcagc tgtaactgct
     4321 gacgccactg gcgaaaactc acccccgtct ctttcactat cagtcgggca agattgcgtt
     4381 cactcatcgc aaaaaagcga gcccattgcc ccaatgaccc ccactcgacg ggctctttcg
     4441 ccatcatctc caccatcgcg cggattttag gatgagaaga aacgggtagg tgcagctgtt
     4501 gttgtggctg ctgagggagt tcatcaaaca ggacctgaat taatctgcgg gtcatcggct
     4561 cagcacgctg agtcgtcgtc ctgtttgcca gggttaaaat taattcccgg cataagggcg
     4621 atattttcaa tgtgcaacag gttgtcggca ttgtcactgc gctgggttcg ataaacagaa
     4681 aacagagttc agcgttagct gtcacttgat tgctatgttc cacccctccc ggtatccaaa
     4741 ccgcatattg tggcggcacc atccacaaag cattttccac cgtacaggta attgcaccat
     4801 gtagtgccag tatcaactgt cctttgcggt gctgatgtac aggactggtt aactcatccg
     4861 ttccggcgtg aatacaaaat gcgaccgcgg cttcatggtg tcggtcgggt tcatagccat
     4921 tgagattcaa cctgtgcatc attttgtccg aatttagcga taatttgtca ttttagcttg
     4981 attcaacata acaataaaaa cggtaaggta cagcctcgtt tgtaacaatg agaagcatat
     5041 gacctgttca acttcattaa gcggcaaaaa caggattgtc cttatcgctg gcattctgat
     5101 gattgccaca acattacgcg tcacctttac cggcgcagca ccgttactgg atacgattcg
     5161 ttccgcttac tcgctgacga cggcgcaaac cggcttattg accaccctgc cattattagc
     5221 ctttgcgcta atctcacctt tggctgcccc ggtggcgcga cgttttggta tggaacgtag
     5281 cctgtttgcc gcgttacttt tgatctgtgc tggtatcgca attcgctctc tcccttcgcc
     5341 ttacttatta tttggcggta cagcggtcat tggcggtggg attgcattag gcaatgtctt
     5401 actgccagga ttaattaaac gcgatttccc tcattccgtc gccagactta ccggcgcata
     5461 ttccctgaca atgggagctg cagcggcact gggatcgact atggtcgtgc cgctggcttt
     5521 gaacggtttt ggctggcaag gcgcgttgct catgctgatg tcttttcctc tgctggctct
     5581 ttttttatgg ctgccacagt ggcgaagtca acaacatgca aatttgagta cctcacgcgc
     5641 cttacatact cggggtatct ggcgttcgcc gcttgcctgg caggtaacat tgtttcttgg
     5701 gatcaactca ctggtctatt acgtgattat tggctggctt ccggcgattc tcatcagtca
     5761 cggctatagc gaagcacagg cgggttcact gcatggtttg ctgcaactag ccacagcagc
     5821 tcccggtttg ctgatcccac ttttcttaca tcatgtgaaa gatcagcgtg gtattgcagc
     5881 gttcgttgcc ttgatgtgcg cagtgggcgc ggttgggctc tgctttatgc cagcacacgc
     5941 gatcacctgg actctgcttt tcggttttgg ttccggcgca acaatgatac tggggttgac
     6001 gttcattggt ctgcgggcaa gttctgcgca tcaggcggcg gcactctcgg ggatggcaca
     6061 atccatcggg tatttgctgg cagcctgtgg gccgccgctg atgggtaaaa tacacgatgc
     6121 taacggtgac tggtccgtac cactgatggg tgttgcaata ctttcactac tgatggcgat
     6181 tttcggactt tgcgccggga gagacaaaga aattcgctaa tatccggtgc tatagtgacg
     6241 tggcaaatca tgcgtgaaag ggagaacaaa tacgatgaat attcagtgca aacgcgttta
     6301 tgatccggct gaacagagcg atggttatcg cgtactggtc gaccgcctct ggccacgcgg
     6361 tatcaaaaaa accgatttag cccttgatga atgggataaa gaaatcacgc cgtcaacgga
     6421 gctgcgcaaa gcctttcacg gcgaagtcgt cgattttgca acctttcgcg agcaatatct
     6481 tgcagaactg gcgcaacacg agcaagaagg aaagcggctg gcagacatcg ccaaaaaaca
     6541 gccgctgacc ctgctctact cagccaaaaa caccacgcag aaccatgcgc tggtgctggc
     6601 cgactggcta cgtagcttgt gattttagta cagcatccgg cggttatttt tcaccagccg
     6661 gatggtcccg tcgccacaat gcccattcat caatcgtttc accgcccggt aatttgcaat
     6721 tgttgctgac cccttgcgct gtctgcactg gaatgagcgt cccgcccttc tgctggcaat
     6781 agaccgacgc cggatttgcc ataccaatct gcggcggttt aggtgcttct ggctgagaag
     6841 aggttgaaca accagccaga accagcaagc aaggcagaac aaaactgata attttcattt
     6901 attgatctca catatttatc caggcttaga gtatcgcggt atcgttttgt tttgcagcac
     6961 tatttttatt acattcactc aaatcatatt acgccttgtt tcatctttgt tgatgatgtt
     7021 ttatcatgcc tgcaaagatt aaataatcag catttacccg ccgtatcctg gagttgtttc
     7081 gtgtcagatc agattattgc ccgcgtctcg caatcccttg ccaaagaaca gtcactggaa
     7141 agcctggtcc gacagcttct ggagatgctg gaaatggtca ctgatatgga gtcaacctac
     7201 ctgaccaaag tggatgtcga agcgcgcctg cagcatataa tgtttgcccg taacagccag
     7261 aaaatgcaca tcccggagaa ttttaccgtc tcgtgggatt actcgttatg caaacgcgcc
     7321 attgatgaaa actgcttttt cagcgatgaa gtccctgacc gttggggcga ctgtattgcg
     7381 gcacgcaatc ttggcatcac cacatttctg agcacgccaa ttcacttacc ggatggatca
     7441 ttctatggca cgctttgcgc cgccagtagt gagaagcgcc agtggagtga acgcgcggaa
     7501 caggttttac agttattcgc cggactgatt gcacaatata ttcaaaaaga ggcactggtt
     7561 gaacagctgc gcgaagccaa tgctgcgctg attgcgcaat cgtataccga ctcgttaacc
     7621 gggctaccga atcggcgggc gatttttgaa aacctgacga cgctgttttc tctcgcccga
     7681 catcttaacc ataagataat gatcgcgttt atcgacctgg ataacttcaa attaatcaac
     7741 gatcgttttg gtcataatag tggcgatctg tttctcattc aggttggcga gcgccttaat
     7801 acgctccagc aaaatggcga agttattggt cgtctcggcg gtgatgagtt tttggttgtt
     7861 tcactgaaca acgagaatgc ggatatttcg tctctgcgag aacgtattca acagcaaata
     7921 cgtggagaat atcacttagg tgacgttgat ttgtattatc ccggtgccag tcttggcata
     7981 gtagaagtcg atcctgaaac gaccgatgca gacagtgccc tgcatgctgc cgatatcgcg
     8041 atgtatcagg agaaaaaaca caaacagaaa acaccttttg tcgcgcatcc agcgctacat
     8101 tcctgaggcg tattcacatc cttttgattg gtgataacat gcgaatcggt attatttttc
     8161 cggttgtaat ctttattaca gcggtcgtat ttttagcatg gttttttatt ggcggctatg
     8221 ctgccccggg agcataaaga tgaaaaaaac aacgattatt atgatgggtg tggcgattat
     8281 tgtcgtactc ggcactgagc tgggatggtg gtaacgtcac atctagaaaa tagcaaaggc
     8341 tgcctgtgtg cagcctttgt gcaatttacg cgttaacttt taatctttct gtagataaac
     8401 agcacgacaa tcgcgccaat aacggcaacc acgaagctgc caaaattgaa gccatcgact
     8461 ttaccaaagc caaacagcgt gctgatccat ccgccgacta cggcaccgac tatccccagc
     8521 aggatagtca taaagaatcc accaccatct ttacctggca tgatccactt cgccagaata
     8581 ccggcaataa gcccaaaaat aatccatgac agaatgccca ttgtttcctc acttatctgt
     8641 tttgcattag cgggttagtc gctgataaaa agcatagcac aacatcggga gggcaagatt
     8701 tgtgacgagc atcacggagg atttttttgc gatggcgcag aaattgcgcc atcaacgatc
     8761 agtgataatt accaaccaca aacatcatgt tcgttttccg tgtcataaga acgcacggta
     8821 ttcaccagat cttttatcac ttcagccgcc acttctggca ccagcaaagt catcggcgtc
     8881 tctgtttcat aatcgacaga aacgccatta ctgttattgg tgacggtcac ggtatacgtt
     8941 gctttgccca tgattcattt cccgttatga atgacttttc gttgttgcgc gccttccatc
     9001 aggacttcag gagcaacgaa gaagtcaatg ttgaaataag tgtcgtcagt catggcttca
     9061 atgttgtgcc acttttctgg agggaacaca gcaaactgcc ccgcttcgat aaggatcact
     9121 tgatcaggct ctgtactgtg ttcatcagcg tagccgatat atttgaccgc cccatgcata
     9181 acggaaaggc gtgggtaaac ccccgggcgc gttcctttat caagatgacg ttcgaatatt
     9241 ccggcaggcg cagtttgttt attccagaaa ggcgttgagc gcgtatgaat ataattctgt
     9301 gggatttgca gcatcctttt ccctccttcg gtgaatgcgc tgaaaacggt ttatttcagc
     9361 cgtttcaggg tacgcctgat aatttgcatt ttaaatacca tttattggtt actatttagc
     9421 accgtatcag agaagaatca gggaggatta caggtgggaa gcccgcgaca acgcgggctg
     9481 aatgcatcag gactggagcg tcgccagtcg ggcggcgaaa cccacaaaca tcaaaccaat
     9541 cagtgagttg cccactttag ccagtttctt tttggtacgt atgtactgcg tgacaaaagc
     9601 accagaaata atcaggaagc tcaaatagca gaaactcacc agttccagcg tcgtcgccag
     9661 aataaagaat gaaattcccg tatgtggggc attaacatcg ataaactgta cgaaaaacga
     9721 cacatagaac aaaatggctt tcggattagt caggctcaaa attaacgcgc gtttaaaaat
     9781 ggcaccgtat tgaggctcat cggatttggc ctcgttattt ttacccttca gggtcgcgta
     9841 gagaatttta ctccccagat agaacaaata aaatgcacca agataacgta caatgttgaa
     9901 taatatcggg gtggtcttaa ttaatgtcgc cactccagcc catgccagaa acatcaatac
     9961 cgcatcgcca ataaatacac cacaggcagc aagataaccg cctttcatac cgctactgac
    10021 gctatttttg agtacaaaca gggtatttgg ccctggcacc aacacaataa aaatggcccc
    10081 aaccagatag gtccagtaat tcagaacccc gtattcagcg aacacattaa cctctttaat
    10141 tatctttcga tcatgcgcga ttaaaggtga atatgctaac caatctgtag cggcttagaa
    10201 aggagaaaat caggttttaa cccgatatca acccgataat tgaatcatta atcaggcatg
    10261 ctccagtgaa aaattcgggt agtgctcggc aaaatactgg cgtaaaaatt ctaccgttat
    10321 ccgtactttc gctgacgtcg ccagccttga aacataaacg gcccagacat tcgctggctg
    10381 gtaatattcc ggtagcactt gtactaaatg gccactggcg ggaaacagcc tgccgacatc
    10441 gatttttacg tggtccggaa aaacaccgaa ggcgaatatt cctcgccagg cggtagagtg
    10501 aatgaaggaa cagagaatga agtcgtcatt caggaatcgg tatttactcg tcgtggtgtc
    10561 gagcgcattt tgcgttaagc tcaaataagt tcatttgaac cgcctgctgt tggatttgta
    10621 gtttgccgaa tccggactga gcgcgcccag tccggcttcg gaagatttta ctgtggatat
    10681 tcctgcaata aattgtgtaa tgcttccgcc atcaactcac cacgccagcc ggaaatcagc
    10741 tccggcaaat tgttctgcgg tttcagtttc cagtgccagt tcagcagttg gttgatttgc
    10801 cgacgcgatg ccagcaattc ggcgctgatc ttgtgcgttt cgctgacgtc agtaatcagc
    10861 gacttaatcg ctttaaacgc tttacgataa cccggcatgt ccatcaggtt aagcatcggc
    10921 tgcggtaagg cctcttccgg caatgcctgc gctttttcca ccagcgccag caacgtttta
    10981 ccgtggaagc ggatttcgct accagataag cccaggctgt ccagttcgcc cagacttccc
    11041 ggcatataac gcgctaccga ccacaaatgc tcttcacgca cgacaaagtt caccgccaga
    11101 tcgcgctctc gcgccttgcg caatcgccag tcggctaaca gttgcagaca ggccagttgg
    11161 cgtgtgcgta actgccaggc attggtgata tcacgccagg cgtcttccgg cgcaacgact
    11221 tcctgacgac gcatttgcat caggcggcat tcatccagcg ccgcaggtag ccagccggag
    11281 gcctccgttt ctaccataag ctttgcggtg atcggtaaca gataccagac atccgccgct
    11341 gcgtattcac actgacgttc ggtcagcggt ctggccagcc agtcggtgcg cgattcactc
    11401 ttgtccagcg taacgccgga atactcttcc accatggaag cgaaacccca tgacatcggg
    11461 cgtccgcaga aggcagcaag gatttgcgtg tcaatcaagg gttgtggtaa ttcgccaaag
    11521 acattgagga acacttccag atcttcactg cctgcatgaa gaaattttgt gatggacgga
    11581 tcgcgcagga tcgctttcag cggtgaccag tcggtgatcc cgagaggatc aattagcgcc
    11641 agatgctcac catcaaaaag ttgaattaaa cccagctgcg ggtaataagt gcgcgtacga
    11701 acaaattcag tatccagggc tatcgccgga aaggcacgga cggcttcaca caaagaagcc
    11761 agcgcatcgt ccgtggtaat catttggtaa ttcaaattgt attctcttta gtgggcgtca
    11821 aaaaaaacgc cggattaacc ggcgtctgac gactgactta acgctcaggc tttattgtcc
    11881 actttgccgc gcgcttcgtc acgtaattct cgtcgcaaaa tttttccgac gttagatttc
    11941 ggtaactcat cacgaaactc caccagcttc ggtactttat agcccgtgag ctgacggcgg
    12001 caaaaggtca ccagtgactc ttcggtaagc gatggatctt ttttcacaac gaagattttc
    12061 accgcttcac cactggagcc ggaaggtacg ccaacagccg cgacttcctg tacgccagga
    12121 tgctgcatga cgacatcttc aatctcgttg ggatagacat taaaaccgga aaccagaatc
    12181 atgtcttttt tacgatcgac aatgcgcagg aatccttctt catccatcac cgcgatatcg
    12241 ccggtgtgta accagccatt tttgatgatt tcatcggtag catccggacg ctgccagtaa
    12301 cccagcatca cctgcggtcc tttgacacaa agctcacctg gttgacccgg tggtacttca
    12361 ttatcatcat catccaccag tttggcttcc gtcgacggca ccggtaaacc gatgctacca
    12421 ctatgataat caatatcata cgggttaacg ctgaccagcg gcgcacactc ggtcaggcca
    12481 taaccttcca gcagatactg tccagtcagt ttcacccaac gttctgccac cacttgctga
    12541 acaggcatcc ctccgcctgc ggaaagatgc agactggaga aatccagctg ctggaactct
    12601 ttattgttca gcaacgcatt gaacaaggtg ttaacgccag tgatagcggt aaacggatat
    12661 ttcgctaact cttttaccaa ccctggaata tcgcgcgggt tagtgataag caggttctgc
    12721 ccccctagtt cgataaacag caggcagtta atggtcagtg caaaaatgtg atacagcggc
    12781 agcgccgtca ccaccagctc tttgcccgga tgcaacagcg gaccataggt cgcgttaacc
    12841 tgttccaggt tcgccagcat attgcggtga gtcagcatcg cgcctttcgc cacaccagtg
    12901 gtgccgccgg tgtattgcag gaaagctaaa tcttccggca ccagttctgg tttgacgtac
    12961 tgcatccggt agccgttgtg cagggcgcta cgaaatgaaa tggcatctgg cagatggtat
    13021 ttcggcacca gacgcttgat gtatttaaca acgaaattga ctaccgtgcc ttttgccgta
    13081 gatagctggt cgcccatacg ggtcagaata acgtgctgaa cggcggtttt atcaaccact
    13141 ttttccagag tgtgggcaaa gttagacacg ataacaatcg ccgatgcgcc gctatcgtta
    13201 agctgatgct caagctcacg cggggtatac aacgggttaa cgtttacgac gatcatcccg
    13261 gcacgcaaaa tgccaaacag cgccaccgga tattgcaata agttaggcat catcaacgca
    13321 acgcgatcgc ctttcttcag ccccaaccct tgttgcaaat aagcggcaaa cgcgcgactg
    13381 cgttcttcca gtttgcggaa ggtcatcacc tcccccatat tcacaaacgc gggttgatcg
    13441 gcgtagcgcg cgaccgactg ctcaaacata tctaccagag attgataacg gtcagggttg
    13501 atctccgtcg gaacgtccgc gggataacgg ttaagccaaa ccttcttcaa ttcttcacct
    13561 ctaaaatgcg tgttcgtcgt catcgcaacc ccaaatgata tacatgcctt taacataata
    13621 ttaactcatc ataccagctt gataattacc caacgaaaag gttgcgaagc gcgtcactat
    13681 ttatttttat ctttaccgta agaatgcaga aacagcggac cagccgctgt ttctttttct
    13741 ttaaaaacaa gcaattattc agttacaaca gtttgtactc tcgcgggacc aggattatac
    13801 cagccccatc caccgtagcc atagggccag ccacgaccgc cataaaacca tggatcaatc
    13861 ggctgaggcg gcataatcac ctgctgggtt aaatgccaac gtttgtaacc cgttacttgc
    13921 atcaccataa acttataggg cgtgttgccg attttgccgt caaccgcacc ggtgattggt
    13981 ccaactaccg tgaccaattg tccacggaaa tccaccgggt ccagaaaacc gttcacatcg
    14041 gcataaatgc gaccgcgaga aggttctccc agcgtcggtc tggctccgct gtccagtggt
    14101 acggtggcaa tttccaggcg ggttttccct tgctggtttt gtaccgcaac cactttgccg
    14161 ccaaagcgag cctcctgacc aacgtacagc tgcggcgcac tcatcacccg aactaaatct
    14221 tgttgcggcg tggaactgct gcctttaatg gcgtccggca cagtgacaca accgctcagc
    14281 attagcgcaa acgtacctgc cagtatgcct ttgataacat tcttttgaac cgccatgatg
    14341 cgactccttt ttttctcagg gcatactctt aagattcatt ctttgcccgg aagtttcttc
    14401 catgcgacgt tgttacgtaa ataaactggt tcggcatgtt ccaccgccac cgttttaccc
    14461 tcggcaaaca tctggcacgc aatcggcagc atatcttcgg cggcaggcag taacacatcg
    14521 ccatcgcgca gggccaaccc gctctcttta ccgagatccg gccaggcttg ccagcccgtc
    14581 cctaccgtca cccattcgcc ggaaagctgt tgcattcgtt catgaaccag ttcgggtttg
    14641 agtacggctt cggtttcttc accatgccag ataccgtttt catcacgctg atattcggcc
    14701 cagtaaactt cgcccattcg cgcgtcaata gctgccagca cgcgggttgc gccgtttttg
    14761 cgccacgccc cttgcgccat cgtcattagc gtggagacgc caatcatcgg cagctctgcg
    14821 ccaagtgcca gcccttgcgc aatgccaata ccaatgcgca cgccggtaaa gctaccgggg
    14881 ccgcgcccgt aagccagagc gtttatgtca gtcagagaag ttccgctggt ggtcaggata
    14941 tcctgcacca tcggtaagat tcgttgagta tgttcacgag ggcaaagctc aaaatgagcg
    15001 ttgacagtac cgtcgttcca cagggcgaca gagcaggcct ctgtcgcggt atcgatagcc
    15061 agaattcgca tgggtcttcg tgcttagatc aataaaaagg cgcgcatcat accatactcc
    15121 gtaacaaatt acctggagga tggtatcgca aggaaacgaa ccgcacgggc aatgtcacgg
    15181 gtgcgcggcg ctggcggcag actggcgaga aacgtcgcgc cgtaagggcg catcaccagc
    15241 cgattatcgc aaatcaccag cacgccgcga tcgtcggcgt cgcgaatcag tcgccctact
    15301 ccctgcttga gagtaatcac cgcatccggc agttgtactt catcgaacgg gtcaccacca
    15361 cgcaaacggc aatcttccat gcgcgctttt aatagtggat catccggtga ggtaaacggc
    15421 aacttgtcga taatcaccaa tgacaatgta tcgccacgca catccacccc ttcccagaag
    15481 ctgctggttg ccacaagaag cgcgttaccg gcgctgacaa attgctgcaa cagttgccct
    15541 ttgctggttt ccccctgcaa caaaacggga agcgtcatgg tagcgcggaa ctgctcagcc
    15601 agatcgcgca tcatggcgtg cgaggtacaa agcataaaac aacgaccgtt gttagcttcg
    15661 atgatcggtc gcaacattgc cgccagttgc cgtgcggagc ccggttgatt ggtttgcggc
    15721 aggttgcgcg gcacacagag taacgcctgg cggctgtaat caaacgggct gggtaacagc
    15781 aacgactctg cctgttcaat gccaagccgc gaggtaaaat gatgcagatc gtcgttcacc
    15841 gacagcgttg ctgaggtaaa gatccagcta ccgggttttt gcgccattaa ctctttgaat
    15901 ttatccgcca cgctgagcgg cgtgagcgcc agagtaaaat ggcgcgaagt gcattcgtac
    15961 cagtagctgt agcccggctg attgatctct tttagccgtt tcagccgcgt acgatacaac
    16021 gtggcgcgct caaatgccgc atccagcaag gcggaacgcc ccagcgacag tttcgccacg
    16081 tcataacaaa gttccagggt gtcatcgagc agtaaaaacg cccgttgaat ttgcggatta
    16141 gctaacagtt cgcgcaggtt gccacgataa ccaggctcac cgagttgcag acgaaaatcc
    16201 tgcgcgctct gggcaaggcg gtcggcgcac ttttgtaact gctgagtatc ttttaattcg
    16261 gtgcgatagg cgatggtgat gtcttttgcc aggtcgagta gttgtcgact ggagagtgac
    16321 tgaccaaaat actggctggc aatgtcaggc agctggtggg cttcgtcgaa gatcatgacg
    16381 tcagcttccg ggatcagttc gccaaatcca ctctctttca ccaccatatc cgccagaaag
    16441 agatgatggt ttaccaccac cacatcggcg tccatcgctt ttttgcgtgc tttgaccaca
    16501 aagcaatctt tatacatcgg gcagtcgctg ccaaggcagt tatcgttggt gctggtgacc
    16561 agcggccacg cctgtgaatc ttccgccacg ctgacgcagg tgctgatatc accatcgatt
    16621 gtttgattag accaggagcg cagcaggatc acatcgctta agatttgtac cggcagatcg
    16681 ccccccgcca gcgcctgctg ttcgagacgt tcgaggcaga ggtagtttga gcgccctttc
    16741 agcaacgcca cgttgcccgt atatttcaac gcctttgaaa ccgttggcaa atcgcggctg
    16801 tagagctgat cctgcaacgc ttttgagccg gtcgagataa tgactttctt tttcgcccgc
    16861 agcgcagggg ccaggtaagc gtaggttttg cccgtaccgg ttccggcttc caccaccagc
    16921 ggctggcctt tttctatcgc ctgggtgacg gctaccgcca tctgtcgctg tggttctcgc
    16981 ggcttaaagc ctggtatcgc tttcgccagc tgaccgtctg gtgcaaaatc gtccgtcaca
    17041 ctaccccctg gtgtgtttat tttgtggttt actcttcccc cgaaggctct gacgggaacc
    17101 atgcagcctg cctccccctg gggttacaag cccgct
//
LOCUS       DAZFJL010000101        16794 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZFJL010000101 DAZFJL010000000
VERSION     DAZFJL010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.101"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            51..1283
                     /gene="rbsK"
                     /locus_tag="ACHJ8C_003305"
     CDS             51..1283
                     /gene="rbsK"
                     /locus_tag="ACHJ8C_003305"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO5553108.1"
                     /translation="MYRDTRREHIVRLLRNKDECSVSDLANYFSVTKETIRTDLAWLQ
                     KRGVVSRHHGGVSLKKHLMQNALSQQEYVDMSLLIKQQQQGIGYLTSLDDKGRRMVGK
                     VCVLGSFNVDIVAKVYRFPNEGETLVARETTMGPGGKGANQALAAHRAGAQIHFACKI
                     GRDQFNHFARNHIESIGIDSFTFYESELSTTGCAVIYVNDEGENMIAMSPGANHELSD
                     NDIIQLSHFIAESDVFIVQMENNLAATQLALKCAQKMQVTTILNPAPWSSDVATLLPF
                     VDIVTPNETEASAMSGMVIDNISDAVKAAKHIYNAGQCSVIITMGKRGALIYDGQQCA
                     HIPAFSAVAVDTTGAGDAFNGALAAALAKGESLVKAAWYASAFASLAVEKEGAANMPD
                     ISLVDARIQQQNVAIQTF"
     gene            1309..2232
                     /locus_tag="ACHJ8C_003306"
     CDS             1309..2232
                     /locus_tag="ACHJ8C_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HFO5553109.1"
                     /translation="MKTIEGIIPVMLTPFTADNKIDYLGLKRLIDWYIENGADALFAV
                     CQSSEMQYLTLEERVELASFVVEYSQQRVPVIASGHISDDLEQQIQELNAMAATGIDA
                     LVLVTNHLDPHNEGTTVFLRTLDALLMSLPESMALGLYECPAPYRRLLTDEELLYCAQ
                     SGRFQILKDVSCDLPTVTRRVQLTQHTPMTIVNANAAIAWEAMLAGSKGFCGVFTNFH
                     PDLYHWLWLEGKQHPEEAKQLAWFLSLSAVSENIGYPKNAKIFHQQRGTFDCVHCRVT
                     ADDVLKKYWGLEMVLKQIADAADFQRQQLKY"
     gene            2287..3240
                     /locus_tag="ACHJ8C_003307"
     CDS             2287..3240
                     /locus_tag="ACHJ8C_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HFO5553110.1"
                     /translation="MKRTLMSCAVASLLVSGSALAAWPEKPIQIIVPWGAGGNTDTVA
                     RLVAEGLQKELKVNVNVVNRTGGAGVVGHDAIAKAKADGYTLGIATVEITMMHHQGMT
                     ALTYKNYTPITRLAVNLGGVQVAANSQFENIVQMTDYIKANPGKLKASGSGLNSGWHL
                     NLIGMLDSMGLPPSAVTWVPSEGSSSALMELVSGGIDFTTSSPGEAKSMVDAKMVRNL
                     ATMAEKSAGLYKDLPVFQQATGYKYHLATWNALVVPAGTPEEVSQKLTAAMQAVFAQG
                     KLQEFATKQGFEVYPLYGKEMTTFMADEDARYGKLITKIKG"
     gene            3289..3726
                     /locus_tag="ACHJ8C_003308"
     CDS             3289..3726
                     /locus_tag="ACHJ8C_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HFO5553111.1"
                     /translation="MRIIPVTPLFLLIFAMFIFGWSITQYGGISQYGAGFMPCIISGL
                     LLFLAAIDVAIDIKHRHRNKVSLTLDEVKVLLLVITVVLLFVLLLDILGFVICSFLLL
                     FILMKIRGVRLLHSVIVSISASLIIWFVFARVLLVAFPEGILF"
     gene            3736..5223
                     /locus_tag="ACHJ8C_003309"
     CDS             3736..5223
                     /locus_tag="ACHJ8C_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HFO5553112.1"
                     /translation="MDILIQALTYINLNAILAIISATAFGLFVGAIPGLTATMAVALM
                     VPFTFFLDPIPALALMIAVGASSIYAGDIPGALLRIPGTPASAAYVADSNELVKQGKV
                     NRVLGLGLVSSVIGGVIGSLILMLAAPVLAKFALKFSSYEYTWLSLLGLTCATLVVGN
                     QPLKGMVTLCLGLLLACIGYDQISGVPRFTFGQISLLQGVNFIPAMIGLFAISGAIEY
                     YRDKISQVSGAVPQTQTGINLFKGMGQVIWSRKLNLGRSSIIGTLIGALPGAGADIAA
                     WISYAISKKFSRQQELYGHGSEEAIVDSSSSNNASLAGSWIPSLVFGIPGDSAAAIII
                     GVLYMKDMNPGPTLFLFQADKLYAVFILFLIANIALLPLATIAVSFIKRIIWIDKAIL
                     YPIILIFSIVGAFAIDNSGASVVVMLVMGVLGYWLQRKEYPVSPIILGMILGPMLEKN
                     LLSSMIKSNGEWLAFVERPVSMALAVCFFLVVALQGRNIYRSFSR"
     gene            5407..6171
                     /gene="yabI"
                     /locus_tag="ACHJ8C_003310"
     CDS             5407..6171
                     /gene="yabI"
                     /locus_tag="ACHJ8C_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO5553113.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLVTAVLLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(6285..6983)
                     /gene="thiQ"
                     /locus_tag="ACHJ8C_003311"
     CDS             complement(6285..6983)
                     /gene="thiQ"
                     /locus_tag="ACHJ8C_003311"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO5553114.1"
                     /translation="MLKLTDITWRYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQKKMHAIAHQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSSSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGIKG"
     gene            complement(6967..8577)
                     /gene="thiP"
                     /locus_tag="ACHJ8C_003312"
     CDS             complement(6967..8577)
                     /gene="thiP"
                     /locus_tag="ACHJ8C_003312"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO5553115.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWV
                     SVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRQMLLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLWQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGCHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMICCLGLVLLSQRLSKAIAPGTTLLQ
                     GWHDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKIPGRNVKTD"
     gene            complement(8553..9536)
                     /gene="thiB"
                     /locus_tag="ACHJ8C_003313"
     CDS             complement(8553..9536)
                     /gene="thiB"
                     /locus_tag="ACHJ8C_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO5553116.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     TEVVNVPGGWKNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYEDPRT
                     STPGLGLLLWMQKVYGDNAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAATNFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(9567..9666)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(9700..11355)
                     /gene="sgrR"
                     /locus_tag="ACHJ8C_003314"
     CDS             complement(9700..11355)
                     /gene="sgrR"
                     /locus_tag="ACHJ8C_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO5553117.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQETTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVISSLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     gene            11444..11575
                     /gene="sgrT"
                     /locus_tag="ACHJ8C_003315"
     CDS             11444..11575
                     /gene="sgrT"
                     /locus_tag="ACHJ8C_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO5553118.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(11683..12288)
                     /gene="leuD"
                     /locus_tag="ACHJ8C_003316"
     CDS             complement(11683..12288)
                     /gene="leuD"
                     /locus_tag="ACHJ8C_003316"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO5553119.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(12299..13699)
                     /gene="leuC"
                     /locus_tag="ACHJ8C_003317"
     CDS             complement(12299..13699)
                     /gene="leuC"
                     /locus_tag="ACHJ8C_003317"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO5553120.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(13702..14793)
                     /gene="leuB"
                     /locus_tag="ACHJ8C_003318"
     CDS             complement(13702..14793)
                     /gene="leuB"
                     /locus_tag="ACHJ8C_003318"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO5553121.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(14793..16364)
                     /gene="leuA"
                     /locus_tag="ACHJ8C_003319"
     CDS             complement(14793..16364)
                     /gene="leuA"
                     /locus_tag="ACHJ8C_003319"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO5553122.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(16456..16542)
                     /gene="leuL"
                     /locus_tag="ACHJ8C_003320"
     CDS             complement(16456..16542)
                     /gene="leuL"
                     /locus_tag="ACHJ8C_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO5553123.1"
                     /translation="MTHIVRFIGLLLLNASFLRGRRVSGIQH"
BASE COUNT         3895 a         4174 c         4447 g         4278 t
ORIGIN      
        1 cccgataaca atttcttttt tagtgataat aaaatcaaaa aggaagactt atgtatcggg
       61 atacccgtcg tgaacatatt gtccggttac ttcgcaacaa ggatgagtgc agtgttagcg
      121 atttagctaa ctatttttct gttaccaaag agacgattcg gacagattta gcgtggttgc
      181 aaaagcgtgg tgtagtgagt cgtcatcatg gaggcgtctc attgaaaaaa catttaatgc
      241 aaaatgcgct ttcgcaacaa gagtatgtcg atatgagttt gcttatcaag caacaacagc
      301 agggtattgg ctatttaacc tcgcttgatg ataaaggacg ccgaatggta ggtaaagttt
      361 gtgtgttagg atcatttaac gtcgatattg tggcgaaagt gtatcgcttc ccgaatgaag
      421 gcgaaacgtt agtggccagg gaaactacca tggggccggg gggtaaaggc gcgaatcagg
      481 cactagccgc tcatcgggca ggtgcgcaaa tacacttcgc ctgtaagatt ggtcgcgatc
      541 agtttaatca ttttgcccgc aatcatattg agtccattgg tattgattca tttacttttt
      601 atgagtctga actatctacc accggttgtg cggtcattta tgtaaatgat gaaggtgaaa
      661 acatgattgc tatgtctcca ggggcaaatc atgaattaag tgataacgat attattcaac
      721 tttctcactt tatagccgag tccgatgtat tcattgtaca gatggaaaat aatcttgccg
      781 ctactcaact ggcattaaaa tgtgctcaaa aaatgcaggt gacaacgatt ttgaatcctg
      841 caccgtggtc cagtgatgtc gccacattat taccttttgt tgatattgtg acgccaaatg
      901 aaaccgaagc atcggcgatg tcaggtatgg taattgacaa tatttctgat gctgtgaagg
      961 ctgcgaagca tatttataat gccgggcaat gttcagtaat tattacgatg ggaaaacgag
     1021 gtgcattaat ttatgacggg caacaatgtg cgcatattcc ggcattttcg gcagtcgctg
     1081 tagatacaac aggtgcgggt gatgcgttta atggcgcgct cgccgccgca ctggcgaagg
     1141 gagaatcact tgttaaagca gcctggtatg ccagcgcttt cgcttcgctg gcagtagaaa
     1201 aagaaggggc agccaatatg cctgatatct ctctggtgga tgccagaatt cagcagcaga
     1261 atgttgccat tcagactttc tgatgtttca ctgaacgaag gatgaaatat gaaaacaatt
     1321 gaagggatta ttccggtaat gctaacgcct tttacggcgg ataataaaat tgattacctg
     1381 ggtcttaaac ggctaattga ctggtatatc gaaaacggtg ccgacgcttt atttgccgtg
     1441 tgtcagtcca gtgaaatgca gtatttaact ttagaagagc gcgtggagct ggcgagcttt
     1501 gtggttgaat atagccaaca acgagtaccg gttattgcgt ccgggcatat ttctgatgat
     1561 ctggaacagc aaattcagga gctcaatgct atggcggcaa cgggcattga tgcgctggtg
     1621 ttagtgacga atcatcttga tccacataac gaaggcacca cggtattcct ccgtacgctt
     1681 gatgcgctat tgatgtcatt acccgaaagt atggcgctgg gtctgtatga atgcccggcc
     1741 ccgtatcgcc gcttattaac cgatgaggaa ctgctttact gcgcacaaag cgggcgtttt
     1801 caaatcttaa aagacgtcag ttgtgatcta cctacagtga cgcgacgggt gcaattaacc
     1861 cagcacacgc caatgacgat tgttaatgct aatgctgcca ttgcctggga ggcgatgctt
     1921 gcaggttcga aaggtttttg tggcgtcttt accaactttc atcctgactt gtatcactgg
     1981 ctttggcttg aaggaaaaca acaccctgaa gaggcaaagc aactcgcctg gtttttatcg
     2041 ctgagtgccg tcagtgaaaa tattggctat ccgaaaaatg ccaaaatatt tcatcagcag
     2101 cgaggaacat ttgactgtgt tcattgccgg gtaacggctg acgatgtgct gaaaaaatat
     2161 tgggggctgg agatggtgct aaaacaaatt gctgatgccg cagatttcca gcgtcaacaa
     2221 ctcaaatatt aatatccgac agcacttgct gttattaaaa aataattaag acaggagtct
     2281 acaaatatga aacgaaccct gatgagttgt gccgttgctt cactgctggt ttcaggttcc
     2341 gcgctggctg catggccaga aaaaccaatc caaatcattg tcccctgggg cgcgggagga
     2401 aataccgata ctgttgcacg cctggtggca gaagggctgc aaaaagagtt gaaagtgaat
     2461 gtcaacgtgg tgaatcgcac gggaggtgct ggggtggttg gccatgatgc cattgcgaaa
     2521 gctaaagcgg atggctatac cctgggaatt gcgacggtag aaatcaccat gatgcaccat
     2581 caggggatga cggcgttaac gtataagaac tacacgccaa ttacacggct ggcagtgaat
     2641 ctgggaggcg tgcaagtcgc agcaaattcg cagttcgaaa atatcgtcca gatgaccgac
     2701 tacatcaagg cgaatccagg taagttaaaa gcatcgggta gcgggttaaa ttccggctgg
     2761 catctaaact tgataggtat gctcgacagt atggggcttc cgccttctgc cgtgacctgg
     2821 gtgccttctg aaggttccag ttctgcactg atggaactgg tttccggtgg tatagatttt
     2881 acaacgtcat caccgggcga agcgaaaagt atggtcgatg ccaaaatggt gcgtaattta
     2941 gcgaccatgg cagaaaaatc ggcagggctt tataaagatc ttccagtatt ccaacaagca
     3001 actggctata aatatcattt ggcgacatgg aatgccttag tggttccggc gggtactcct
     3061 gaagaggtct cacaaaaatt aactgccgct atgcaggcag tgtttgcgca ggggaaatta
     3121 caagaattcg ctaccaaaca gggttttgaa gtttacccgc tgtatggcaa agagatgacg
     3181 acatttatgg cagatgaaga tgccagatac ggaaaattaa taaccaaaat caaaggttaa
     3241 tcagacaggg cggtgaatat atcgccgcca aattacaggg atagcattat gcgcataata
     3301 ccggtaacgc cgttatttct gctgattttt gccatgttta tttttggctg gagcataacg
     3361 caatatggag gcatatcgca gtatggcgct ggatttatgc cttgcatcat cagtggattg
     3421 ttgttgtttt tagcggctat tgatgtagcg atagatatta aacaccggca ccgtaacaaa
     3481 gtttctttaa cacttgatga agtgaaagta cttttactgg ttattacagt ggtactgctg
     3541 tttgtattat tgttagatat tctcggtttt gtaatttgct catttttgtt gctttttatt
     3601 ttaatgaaaa tacgaggtgt cagacttttg cattccgtta tcgtttcgat atcggcatct
     3661 cttattatct ggtttgtttt tgccagagtg ttgttggtcg cctttcctga aggaatcttg
     3721 ttttaaggat gcaatatgga tattttgatt caggcactaa cgtatattaa tttgaatgcc
     3781 attctggcca ttatttctgc tacggccttt gggttatttg tgggagccat tccaggattg
     3841 accgcaacga tggcggttgc gctaatggta ccttttacct tctttcttga tcctattccc
     3901 gctctggcat taatgattgc cgttggtgcg tcatccattt atgccggaga tatccccggc
     3961 gcacttttgc gtataccagg gacacctgct tcggcggctt atgttgctga ttcaaatgaa
     4021 ctggtaaagc aaggtaaggt gaatcgcgta ttgggacttg ggctggtgag ttcggtcatt
     4081 ggtggcgtga ttggatcact tattttgatg ttggctgcgc ctgtactggc gaaatttgcc
     4141 cttaaattta gttcttatga atacacatgg ctttctcttt taggcttaac ctgcgcgact
     4201 ctggttgtgg ggaatcaacc actgaaaggc atggtgacct tatgtttggg attattactg
     4261 gcgtgtatcg gttatgatca aatatccggg gtcccacgtt ttacgtttgg gcaaatcagc
     4321 ctgttacaag gggtgaactt tattcctgcg atgatcggtc tgttcgcaat atccggcgca
     4381 attgaatatt atcgtgacaa aattagccag gtaagtggtg ctgtgccaca aacgcaaacg
     4441 gggattaatt tattcaaggg aatggggcag gtgatttggt cacgaaaatt aaatcttggc
     4501 cgaagtagta ttatcggtac gcttatcgga gcattgcccg gcgctggagc ggatattgct
     4561 gcgtggataa gttacgccat ttcaaaaaaa ttctctcgcc agcaggagtt atatgggcat
     4621 ggatctgagg aggcaattgt tgattcctcg agcagtaaca acgccagtct ggcggggagt
     4681 tggatccctt cattagtttt cggtattccg ggcgattctg ccgctgcaat tatcatcggc
     4741 gtgctctata tgaaggatat gaatccaggg ccgacattat ttctgttcca ggcagataaa
     4801 ctctatgccg tatttatctt gtttctgatc gccaatattg cattactacc gttagccaca
     4861 attgcggtca gtttcatcaa acgcatcatc tggattgata aagcgattct gtacccgatt
     4921 attctgattt tcagtattgt cggtgcattt gctatcgata actcgggtgc ctctgtcgtt
     4981 gtcatgttgg tcatgggggt tcttggatac tggttacaac gtaaggaata cccggtttcg
     5041 cccattattc tggggatgat tcttgggcca atgttggaaa aaaacttatt atcttcaatg
     5101 attaagtcta atggtgaatg gctggcgttt gttgagcgcc cggtatctat ggcactggct
     5161 gtctgtttct ttttggttgt cgcgttgcaa ggacgcaata tctaccgttc atttagccgt
     5221 taatccgttg ttttcagacc atccaagttg tggatggtct gccttcccct aagtaaaatc
     5281 ctgcgtgact gctaaattta tactctaaat gattcgcgtt gcaggagtat attcatacaa
     5341 agaatagatt gacctgaata ccaccacttt gcacaatcca cgacgacttc atcaaccggg
     5401 cacattatgc aagcattgct ggaacacttt attacccaat ccaccgtgta ttcattgatg
     5461 gcggtggtgt tggtggcctt tctggagtcg ctggcgctgg tcggtttgat tctacccggt
     5521 acggtgctga tggcggggct gggtgcgctg attggcagcg gcgagttaag tttctggcac
     5581 gcctggctgg cagggatcgt cggttgcctg ctgggcgact ggatctcctt ctggctgggc
     5641 tggcgtttta aaaagccgtt gcatcgctgg tcatttctga agaagaacaa agcactactc
     5701 gataaaactg aacatgcgtt gcatcaacac agcatgttca ccattctggt cggtcgtttt
     5761 gtcggcccga cgcgtccgct ggtgccaatg gtggcaggga tgctggatct gccggtggcg
     5821 aaatttatta cgccaaatat tatcggctgc ctgttatggc caccgtttta cttcctgccg
     5881 gggattctgg cgggtgcggc gatcgatatt cctgccggga tgcagagtgg tgagtttaaa
     5941 tggttgctgc tggtgacggc ggtattgttg tgggttggtg gctggctgtg ctggcggtta
     6001 tggcgcagcg gtaaagcgac tgaccgtttg agtcattatt tgtcccgcgg tcgtttgttg
     6061 tggctgacgc cgttgatttc tgccatcggc gtggtggcgc tggtggtgtt aattcgccac
     6121 ccgttgatgc cggtgtatat cgatattttg cgtaaagtgg ttggggttta gaggatagcc
     6181 ttgtgcgggt tgcctgatgc ggcgcttgcc gcgtcttatc aggcctacaa aacgcactac
     6241 ccgtaggtcg gataaggcgt tcacgccgca tccgacagta catattaacc tttaattccc
     6301 aacagcgccg aagcactcgc cttaccgctc aacaactcat cggtcttacc ctgccaggcg
     6361 atacgcccgt cggcgaccac caccgagcgc gtggcgatcc gcgccgcatc ttccacactg
     6421 tgcgacacca tcaagagcgt cattttttgc tgttggcagc tcgaactcac cagcgtcagc
     6481 atctcctgac gtagcgccgg atcgagtgcg gaaaacggtt catcgagcaa taaaatgggc
     6541 tgttcgcgta ccagacaacg cgctaacgcc acgcgctgtc gctgaccgcc ggaaagctcg
     6601 ccaggtaacc gcgccattaa attatcaatc cccatctggt gggcaatcgc gtgcattttc
     6661 ttctgctgcg ccgcatttag tttcaacccc gggtttagcc ccagcccgat gttctgcgcg
     6721 accgtcaggt ggctgaacag gttgttctcc tgaaacagca tcgacaccgg acggcgtgac
     6781 ggcggcgtcg tggtgtgatc aacgccgtcg atagtcagcg aaccgctggc tggcgtcaga
     6841 aaaccggcga tcaaattcag cagggtactt ttacccgcgc cgcttggccc gaggatcgcc
     6901 acctgctcgc cgcgttccac cgttaagcta aaacgcatcg gcaaatggtg ataacgccag
     6961 gtgatatcag tcagttttaa catttcgccc cggtattttt tcaatcacgg taaacagcag
     7021 aaaacagagt agtagcagaa ttaacgcggt caccgcgccg tcctggctgc gataggagcc
     7081 aatttgctgg tagagataaa acggtagggt tcggaaatca tcgttaccga acaacgccac
     7141 cacgccaaaa tcaccaatcg acagtacaca ggcaaaggcc agcgcctgcg ccagtgggcg
     7201 tttcagggcg cgcagctcca ccacttttaa gcgtgaccag ccttcaatgc ccagcgactg
     7261 acacaacatg ctgtagcggg cggtgatatc gcgcatcggg ttttccagca ctttcagcgc
     7321 ataagggatg gccattaacg cattggtgaa aatcacgatg ccgtcagcag attgtggcag
     7381 gccgatggtg ttgttgagca gtaaaaagaa gcccgttgcc agcacaatcc ccggcatagc
     7441 gaggatcaac atgccgctca tctccagcgc ctggcccgcc agcatttttt gccgtgcccg
     7501 caattcgcga ctgctccata gcagcatcat ggtcagcacc acgcacaata cgcctgccgc
     7561 cagcgcaata cgcaacgagg tccacagcgc ctgccacagc accggttgtg ccagcacctc
     7621 tggcaactgg cgatttaccc catcgacaat caccgccagt aacggtggca gcaacagcag
     7681 cagcgccagc acaattaaca ccgtgtcgca aatgcggcta tgcagacggt cgtccgggtc
     7741 gtgccagcct tgcagcagcg tggtgccggg cgcaatggct ttacttagtc gctgactcaa
     7801 cagcaccagc ccaaggcaac atatcatctg gatcagcgcc agcatcgccg cgcgggcagg
     7861 atcgtagtcg taactcagcg cctgataaat tgccagctcg atagtggtcg cctgcggacc
     7921 gccgcccagc gatagcacgg tggcgaagct ggcgaaacag agcataaaga ttagtgcggc
     7981 aaccggcggg atttgtcgcc gtaaccacgg ccattcgacg aagcggaaga agtgacagcc
     8041 gcgcatccca agctgggcgg caagctgacg ttgttcgccg ggaatgtttt ccagtgcctg
     8101 gagtaataaa cggctagcca tcggcagatt aaaaaacaca tgggccagca aaatgccttg
     8161 caggccgtag ggcgaaaagg tccactccag cccaagtgat tgccagagcg aggccagcca
     8221 gccctggcga ccatagacgc taagaatgcc gaaaacggca accagcaccg ggaggatcaa
     8281 ggtcattgcg cacagacgca acagcatctg tcgcccagga aagcgcctgc gatagagggc
     8341 gcgggcgagg aatattgcgg gtacgacaga gagcagcgcc gagagaaacg cctgccagaa
     8401 ggagaagcgc accacatgcc acaggtagct gtcctgccag actgacaccc agtcaccctg
     8461 cggcgcgttc caccacaggg cgagaaacgc cgccagtgca accgctacca ccagcgtggc
     8521 ggcgcttacg cctggaatta accagccggg aattaacggc tgacggcgcg ttgccattcg
     8581 ctaatccatg cctgacgttg tgccgccact tcggcgggtg tgaactctag cgtggtggct
     8641 ggtttggtca attgttcaaa tcctgcaggc agagtgacgt ttgctaccgg atacatccag
     8701 ttgccggttg ggatcgcatt ctgaaaagcc ggagaaacca taaactggag gaatttttgc
     8761 gccagctctg gctgcttgct ggcagcggtg cgggcggcaa cttccacctg cagatagtga
     8821 ccttcgctaa agtttgtggc ggcgtagtta tctttcttct cttcgagaat gtgataagcc
     8881 ggagaggtgg tgtaactcag taccagatcg ctttcgcctt ttaaaaacag gccgtaggct
     8941 tcgctccagc ctttggtgac cgtgacagtt ttcttcgcca gtttctgcca ggcttgcggg
     9001 gcgttatcgc catagacttt ttgcatccat agcaacagac ccagccccgg cgtactggta
     9061 cgtggatctt cataaatcac ccgccagttc tgatcgctct caaccagttc tttcaggctt
     9121 tgcggcggat ttttcagttt gttcttgtca tagacgaagg cgaaatagcc atagtcaaac
     9181 ggcacaaagg tgtcattttt ccagccgccg ggaacgttta caacctctgt cgcgacgccg
     9241 cttttggcaa acaacccggt tttgctggcg gcgtccagca ggttgttatc cagccccagc
     9301 accacatcgg ctttgctgtt tttgccttcc atccgtagac ggttgagcag cgaaacgcca
     9361 tcttccagcg ccaccagttt cagttcgcaa ttacagtctg cttcaaaggc ttttttgact
     9421 accggacctg gtccccagtc agcggcaaag gaatcgtagg tataaacagt cagaacaggt
     9481 ttagcgaaaa caggcgctgt gcacagcaac agcaggggaa gacatttttt taacactttg
     9541 cacctcacaa aagagtggca aaggacttga gaaggagcct caaatccctt cgccggcgtt
     9601 atccggatca ggttcgacgg gtattttctc agcgcacgcg gctgcgtggc accccgttga
     9661 gaacggcgtt agtgtagtga ttttgttatc aaccagcaat catggatccg gtggtgcaaa
     9721 ccacgctgat ttaaaatcga accagccgag ggtgttcatg cgcaggccgc gcatactacg
     9781 ttgcccctga atgatcagcc agtggtggat aagtggcacc attgctttgc tggcgaccag
     9841 ttgttggcac cagttcgcca ggttcatttc gccattgcgc cagcgggcgg cgtcggcttg
     9901 ccagtcgatc ggaatacagt gttggagcag cggcacctcg cacaagtgcg cgaacagcga
     9961 aaaatccagc ggcagcgtaa agttggcgct gttaagccag atatcgctct cgatctctcc
    10021 ttcatgccac tgatcgtagc tgatctcttt gatttccagc gtgacctggt gacttgccag
    10081 aatctgctgc atgatcccgg caatcacccg atgctcgctg tgatcctgat aaaaggtcag
    10141 ggtgaggctt tccagaccag ccggtttttc gctctttatg gtgcgggcat ggtgccaacg
    10201 ggggagcagt ccataagccg ggaaccacag ttgctggtac tgttcctcag cgaaatagac
    10261 cagattcgtc ggagaaagca catagcttac ccagtctctg acttgctgat tcgcccctcg
    10321 atgggtgcgg ctgtcgaaca gtaaatagta gcaaccttcc tccaggcggc tttcaatctc
    10381 tttttcctcg ccctgtggac cttttagcat cagccctccg gctggctcgt cggcaatttc
    10441 cggcagaacc cagacgttaa cttcgtcgat taatgcccgg taaccgaaga agtcatcgaa
    10501 tgcctgaatt ttcagttgat tggtgctgtt gcggatcacc gcatacggac cggtgccgat
    10561 gggatggctg gcaaagttac tgagggtttc ccattcgcgc ggcaggatca ttgccggaac
    10621 ttgccccagc agtaacggca accagcggtc cggttgcgtg agatggatat ccagcgtcca
    10681 gggcgtcggc gacacaatgt cagcaatatg cgaatagagc ggaagcgtat tgattcgttt
    10741 taaagaggag atcacatcgt ccatttccag ttcacgacca tggtgaaaat ggacacctgg
    10801 acgcaaaaag aaacgccagt gaagcggtga tatttgctgc cagtggtggg cgatgtctgc
    10861 ttccagttcc ccattttcct catttatgcg cgttagcgaa ctgaagattt gccgggcgat
    10921 atgggtttca gaacggcgca atgcgctgcc aggtagcaga ttacgcaacg gacgatagta
    10981 gagcacgcgc aggatgtgcc gcccctggcg gaagctgcgg cccagatgag aaaccagcat
    11041 ttgccgcaca gtcgctttgt cgccaaccaa ctgcactagt tggtcgatac gatcctgctc
    11101 cagcaggtct tccgcccgct gttgctgaag cgccagcccg gtatagagga atgtcaggcg
    11161 tgaacgttta ccgcgcccga cttccgcttc ccacgtcagc cagccgcgat cctgcatggt
    11221 gttgagcagg gtgcgcatat gacgacgcga gcaactcagt aacgctgcca gttcgttgag
    11281 agtggtctcc tgagatttac cctcgcagca ttgccacagg cggatgaact gttgttgcag
    11341 acgagcagat ggcataaaag gggaactcct gcgcaaaaga cagcaatttt attttcccta
    11401 tattaagtca ataattccta acgatgaagc aaggaggtgc cccatgcgtc agttttatca
    11461 gcactatttt accgcgacag cgaagttgtg ctggttgcgt tggttaagcg tcccacaacg
    11521 attaaccatg cttgaagaac tgatgcaatg ggaggggagt cattctgact actgatttgc
    11581 ggacatcatg tgtgactgag tattggtgtt aatcgccacg ccagcagtga ttatctgctg
    11641 gcttttttct ttttggcgat tgagtgccgg acggtgtaca aactaacgca taaacgccgg
    11701 ttgcttctct tcataagacg caattgcgtc gtcgtgctgc agagtcaggc cgatgctgtc
    11761 cagaccgttc atcatgcagt ggcggcggaa ggcatcgatg gtaaagcgat aagttttctc
    11821 tcccgctttc acctcttgcg cttccaggtc tacatcaaaa tgaatccccg gattggcttt
    11881 caccagcgca aacagctcat ccacttctgc atcgcttaat ttcaccggca gcagctggtt
    11941 gttaaagcta ttgccgtaga agatgtcagc aaaactcggc gcaatcacca ccttaaaacc
    12001 gtaatcggtc aatgcccacg gcgcgtgctc acgcgaggaa ccgcagccga agttttctcg
    12061 tgccagcaaa atggaagcgc cctgatactg cgggaagttc agcacgaagt ccgggtttgg
    12121 ctgttggcct ttttcatcca gaaaacgcca gtcgttaaac agatgcgcgc caaaacccgt
    12181 acgggtcact ttctgcaaaa actgtttcgg gatgattgca tcggtatcga cattggcggc
    12241 atccaacgga accaccaggc ctgtgtgttt gataaatttc tctgccatgg tgtgctcctt
    12301 atttaatgtt gcgaatgtcg gcaaaatgac cggtcactgc cgcggcggca gccattgccg
    12361 ggctgaccag atgcgtgcgc ccgccgcgcc cctggcggcc ttcaaagtta cggttgctgg
    12421 tggaggcgca acgttcgccc ggattcaggc ggtcgttgtt catcgccaga cacattgaac
    12481 aacctggcaa gcgccattca aaaccagctt cgataaaaat tttgtccaga ccttccgctt
    12541 ccgcctgggc tttaaccgga ccggagccgg gaaccaccag cgcttgtacg cctggcgcga
    12601 ctttccggcc tttggcgatt tccgctgccg cgcgtaaatc ttcaatacgc gagttagtac
    12661 aggaaccaat aaacactttg tcgatagcca cttctgtcag cggaataccc ggtttcagcc
    12721 ccatataggc cagcgctttt tctgccgacg cgcgttcaac cggatcggca aacgaagccg
    12781 gatcgggaat attgtcgttc acagaaatca cctggcctgg attggtgccc caggtgacct
    12841 gcggcgaaat ctcttctgct tgcagagtga caacggtatc gaaagttgct ccttcgtcgg
    12901 tttgcagggt tttccagtag gcaacggcgt cgtcgaaatc tttgcctttc ggcgcatgca
    12961 gacggccttt aacatagtta aaggtggttt cgtccggtgc aaccagaccg gcttttgcgc
    13021 ccatttcgat tgccatattg cacagggtca tacgaccttc catgcttaaa tcacggattg
    13081 cttcgccgca aaattccacc acatgcccgg taccgcctgc gctgccggtt ttaccaataa
    13141 ttgccagcac gatatctttt gcggtaatgc ccggcgcggc tttgccctgg acttcaattt
    13201 tcatggtctt cgcccggccc tgtttcaggg tttgcgttgc cagtacgtgt tcaacttcgg
    13261 aagtgccgat accaaaggcc agtgcgccaa acgcgccgtg ggtggcggta tgtgagtcgc
    13321 cgcagacaat ggtcatcccc ggcaaggtca cgccctgttc cggccccatt acgtggacga
    13381 tcccctgata cgggtgattc aggtcataca gctcgacgcc aaattctttg cagtttttga
    13441 tcagctcctg catctggatg cgcgccattt caccgcaggc attaatgtct ttcgtctggg
    13501 tagagacgtt gtgatccatg gtggcgaagg ttttgcccgg ctgacgtacc gggcgaccgt
    13561 gggcgcgcag accatcgaac gcctgcggtg aggtcacttc atgcaccagg tggcggtcga
    13621 tatataacag cggggtttcg ttttcggctt cgtacactac gtgagcgtcg aataattttt
    13681 cgtataacgt cttagccatg attacacccc ttctgccaca tagcgggcaa tgatatcgcc
    13741 catttcatcg gtactaacgg cggcagcgcc acgggctaaa tccccggtgc gaatgccttc
    13801 ttctaatgcg cggttaatgg cgcgttcaat ggcggaagcc gcatcatcgg catccaggct
    13861 gtaacgcagc agcagcgcca gcgacagaat ttgtgcaatc gggttggcga tatttttacc
    13921 tgcgatatcg ggtgccgagc cgcccgccgg ttcatacaga ccaaaacctt gctcgttcag
    13981 gctggcggaa ggcaacatcc ccattgagcc agtgatcatt gcgcactcat cagacagaat
    14041 gtcgccaaac aggttggagc acagcaagac gtcaaactgt gatggatctt taatcagctg
    14101 catggtggcg ttgtcgatgt acatatgcgc cagttcgacg tccgggtatt ccgtggcgat
    14161 ctcgttaacg atctcccgcc ataaaataga ggattgcagc acgttggctt tgtcgattga
    14221 ggtgactttg tggcgacgct tgcgggctga ttcaaatgcg atgcgggcaa tacgctcgat
    14281 ctcaaaacgg tggtacacct cggtatcaaa cgctttttca tattgtccgc taccttcgcg
    14341 gccttttggc tgaccgaaat agatgccgcc ggtcagttcg cgcacacaca ggatgtcaaa
    14401 gccgtttgcg gcaatgtcag cacgcagtgg gcagaacgct tccagcccct gatacagttt
    14461 tgccggacgc agattgctga ataatttgaa gtgtttacgc aaaggcagca gtgcgccgcg
    14521 ttctggttgc tggtctggcg gcaaatgttc ccacttcgga ccacctaccg agccaaacag
    14581 cacggcatcg gcttgctcac aaccttcaac cgtcgcaggc ggcagcggct gtccgtggtt
    14641 atcaatggct gcgccgccta catcgtaatg gctggtggtg atacgcatcg caaatcggtt
    14701 gcgcacggca tccagcactt tcagcgcctg ggtcatcact tccggaccaa taccgtcccc
    14761 cggcaatacg gcaatatggt aattcttcga catcacacgg tttccttgtt gttttcgttg
    14821 tgttgagctt tgcgttgcaa ctctttttcg acttctgcgg cacgccagat attgttcagt
    14881 acgtgcacca tggctttggc ggaggactcg acaatatcgg tggccaggcc gacgccgtgg
    14941 aagcggcgac cgttgtagtt ggcgacaata tccacctgac ccagcgcatc tttaccgtgg
    15001 cctttggcgg tcaggctgta tttcaccagt tcgacgttat agtcagtgat gcggtttatc
    15061 gcctggtaga cggcatcgac cggaccgtta ccgttggcgg cttctgcttt gacttcttcg
    15121 ccacaggcca gtttgacggc ggcagtggca atatcgttag aacctgactg cacgctgaag
    15181 taatccagac ggaaatgctc cggctcttct tgctgcttac cgatgaaggc cagcgcctcc
    15241 agatcgtaat caaacacctg gccttttttg tccgccagct tcaggaaagc gtcgtacagg
    15301 ttgtctaaat tatattcact ttctttatat cccatctcat ccatgcgatg tttcaccgcc
    15361 gcacgccccg aacgagaggt cagattcagc tggatttggt tcagaccaat agattctggt
    15421 gtcatgattt cgtagttttc gcggtttttc agtacaccat cctggtggat accggaggag
    15481 tgtgcgaatg cgccgctgcc aacaatggct ttgtttgccg ggatcggcat attacaaatc
    15541 tggctaacta actggctggt gcgccatatc tcctggtgat tgatggcggt gtggacgttg
    15601 agaatatcct tacgaacttt gatcgccatg atgacttctt caagggaaca gttaccggca
    15661 cgctcgccga tcccgttcat tgcgccttct acctgccgtg ctccggcatg taccgccgcc
    15721 agtgagttgc cgaccgccag gcccaaatcg tcgtgggtat gtacggaaat aatggctttg
    15781 tcgatgttgg gtacgcgttc atacaggccg ctgatgattc cggcgaactc aaatggcatg
    15841 gtgtagccca cggtgtccgg aatgttgatg gtggtggcac cggcattaat cgccgcttcg
    15901 accactcgcg ccagatcggc aattggggtg cgtccggcat cttcgcaaga aaattcaaca
    15961 tcatcggtgt aattacgggc gcgtttcacc atatagatag cgcgttcgat cacttcgtcc
    16021 agcgtgctgc gcagcttggt ggcgatgtgc attggcgaag tggcaataaa agtatggata
    16081 cggaaggctt cggcgacttt cagggattcg gctgccacgt caatatcttt ttccacgcag
    16141 cgagctaacg cgcatacacg gctgttttta acctggcgag cgatggtctg cactgattca
    16201 aaatcacccg gcgaagagac ggggaaaccg acttccatca cgtcaacacc catacgctca
    16261 agggccagcg caatttgcag tttttctttc acactcaagc ttgcctgtaa cgcctgttca
    16321 ccgtcgcgca atgtggtatc gaaaataatg acttgctggc tcatggtttg ggtccttgtc
    16381 tctttttgag cgcctcgctt cgggcataaa aaaacccgcg caatggcgcg ggtttttgtt
    16441 tgactgcgtg ctggcttaat gctggatgcc gctcactcgt ctaccgcgca aaaaagatgc
    16501 gtttagtagt agtagaccga taaagcgaac gatgtgagtc attaaatcag ctccagatga
    16561 atgcgatatg cttttagagt tactggatac aaaaacggat gtcaaccctg aagcaataaa
    16621 aacgtcacgc cattgtgagt tcggcatccg taaaattagc taattgtgct gcggtggtta
    16681 aaacaagcga tattaatttc tgcttaaccg ccgatgcttt tcatcggttg acatatttca
    16741 gaataatttt ttgcatctaa tcaacgaggg aaagggggat aaaatgcacg cgtt
//
LOCUS       DAZFJL010000102        16722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFJL010000102 DAZFJL010000000
VERSION     DAZFJL010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.102"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(213..764)
                     /gene="yghD"
                     /locus_tag="ACHJ8C_003321"
     CDS             complement(213..764)
                     /gene="yghD"
                     /locus_tag="ACHJ8C_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFO5553124.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLTQHLAGRSPREKGMLLAAV
                     VFLFSAGYYVLIWQPLSERIEQQETMLQQLVAMNARLKSAAPDIIAARKSGTTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDVS
                     AAEKPGMVNVQRLEFGRGKVSGD"
     gene            complement(766..1944)
                     /gene="gspL"
                     /locus_tag="ACHJ8C_003322"
     CDS             complement(766..1944)
                     /gene="gspL"
                     /locus_tag="ACHJ8C_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO5553125.1"
                     /translation="MSSMLEIFFPLCAADPIRWQRHTPDVEHGIWPDVADERLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVPRKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSIPCGVMAGDAQRIICRIDECRGWS
                     AGRALAPVMFRAQLNEQNLPISLTVVGIAPEELSAWAGADAERLTVTALPAITTYGES
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALVVERGVTLWSVSEQVAQSRTQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTDLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAADYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(1941..2918)
                     /gene="gspK"
                     /locus_tag="ACHJ8C_003323"
     CDS             complement(1941..2918)
                     /gene="gspK"
                     /locus_tag="ACHJ8C_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO5553126.1"
                     /translation="MITLPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMARQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTILSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(2915..3520)
                     /gene="gspJ"
                     /locus_tag="ACHJ8C_003324"
     CDS             complement(2915..3520)
                     /gene="gspJ"
                     /locus_tag="ACHJ8C_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO5553127.1"
                     /translation="MRRARTGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLNHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHGGYLERLAWPLTDAAGSVKPTTQKLIPADSLRLQFHDG
                     TRWQESWSSVQAIPVAVRIILHSPQWGEIERIWLLRGPQLS"
     gene            complement(3517..3888)
                     /gene="gspI"
                     /locus_tag="ACHJ8C_003325"
     CDS             complement(3517..3888)
                     /gene="gspI"
                     /locus_tag="ACHJ8C_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO5553128.1"
                     /translation="MKRGFTLLEVMIALAIFALAATAVLQIASGALNNQQVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTIPLNTGNVLLQAVDIEVSLH
                     DDFSPVIQSRRAWFSAVGGQQ"
     gene            complement(3885..4448)
                     /gene="gspH"
                     /locus_tag="ACHJ8C_003326"
     CDS             complement(3885..4448)
                     /gene="gspH"
                     /locus_tag="ACHJ8C_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO5553129.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATASEPPAKKAAQAF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALEMQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERVP"
     gene            complement(4452..4907)
                     /gene="gspG"
                     /locus_tag="ACHJ8C_003327"
     CDS             complement(4452..4907)
                     /gene="gspG"
                     /locus_tag="ACHJ8C_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO5553130.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADARNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(4924..6147)
                     /gene="gspF"
                     /locus_tag="ACHJ8C_003328"
     CDS             complement(4924..6147)
                     /gene="gspF"
                     /locus_tag="ACHJ8C_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO5553131.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKDLIPVHIEA
                     RMNASAGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRMLIAM
                     SDALQASGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALADLRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(6147..7640)
                     /gene="gspE"
                     /locus_tag="ACHJ8C_003329"
     CDS             complement(6147..7640)
                     /gene="gspE"
                     /locus_tag="ACHJ8C_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO5553132.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSLELTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCCQSAPATDEEKRLLGITDAHAVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(7640..9700)
                     /gene="gspD"
                     /locus_tag="ACHJ8C_003330"
     CDS             complement(7640..9700)
                     /gene="gspD"
                     /locus_tag="ACHJ8C_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO5553133.1"
                     /translation="MFWRDMTLSIWRKKTTGLKTKKRLLPLMLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWGSKDAGLM
                     QFANGTQIPIGTLGAAISAAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPILPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(9730..10689)
                     /gene="gspC"
                     /locus_tag="ACHJ8C_003331"
     CDS             complement(9730..10689)
                     /gene="gspC"
                     /locus_tag="ACHJ8C_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO5553134.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLSQRQSLNTDKEHLRKIARAM
                     FWLMLLIISAKMAYSLWRYFSFSAEYTAVSASANKPLRADAKPFDKNDVQLVSQQNWF
                     GKYQPVAAPVKQPESAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKMERLSLAEEERPPVAVTSKKAASDEAKQAVAEPVVSAPV
                     EIPAAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARYDISIALR"
     gene            complement(10707..11117)
                     /gene="gspS2"
                     /locus_tag="ACHJ8C_003332"
     CDS             complement(10707..11117)
                     /gene="gspS2"
                     /locus_tag="ACHJ8C_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFO5553135.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISESGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(11183..11992)
                     /gene="pppA"
                     /locus_tag="ACHJ8C_003333"
     CDS             complement(11183..11992)
                     /gene="pppA"
                     /locus_tag="ACHJ8C_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFO5553136.1"
                     /translation="MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASIIDLDNQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(12139..16701)
                     /gene="sslE"
                     /locus_tag="ACHJ8C_003334"
     CDS             complement(12139..16701)
                     /gene="sslE"
                     /locus_tag="ACHJ8C_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HFO5553137.1"
                     /translation="MNKKFKYKKSLLAAILSATLLAGCDGGGSGSSSDTPHADSGSGS
                     LPDVKPDPTPNPEPTPDPEPTPDPTPDPEPTPEPEPEPVPTKTGYLTLGGSQRITGAT
                     CNGESSDGFTFTPGDKVTCVAGNNTTIATFDTQSEAARSLRAVEKVSFSLEDATELAE
                     SNDKKANAVSLVTSSNSCPADTEQVCLTFSSVTESKRFDSLYKQIGLTPDEFKKLVNE
                     EVENNAATDKAPSTHTSSVVPATTEGTSPDLNASFVSANAEQFYQYQPTEIILSEGRL
                     VDSQGYGVAGVNYYTSSGRGVTDENGNFSFSWGETISFGIDTFELGSVRGNKSTIALT
                     ELGDEVRGANIDQLIHRYSTAGKNDTRVVPDDVRKVFAEYPNVINEIINLSLSNGATL
                     DEGDQNVVLPNEFIEQFKTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQIQGVI
                     NKLWGVDKDYKSVTKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKNYWL
                     AFGEKRAWDKNELAYITEAPSLVEPENVTRDTATFNLPFISLGQVGEGKLMVIGNPHY
                     NSILRCPNGYSWNGGVNKDGQCTLNSDPDDMKNFMENVLRYLSNERWLPDAKSSMTVG
                     TNLDTVYFKKAGQVLGNSAPFAFHKDFAGITVKPMTSYGNLNPDEVPLLILNGFEYVT
                     QWGSDPYSIPLRADTSKPKLTQQDVTDLIAYLNKGGSVLIMENVMSNLKEESASSFVR
                     LLDAAGLSMALNKSVVNNDPQGYPDRVRQQREKGIWVYERYPFVDGKPPYTIDETTKE
                     VIWKYQQDNKPDDKPKLEVASWQEEVEGKQVTRYAFIDEAEYTTKESLDAAKAKIFEK
                     FPGLQECKDSTYHYEVNCLEHRPGTNVPVTGGMYVPRYTQLNLSADTAKAMVQAADLG
                     TNIQRLYQHELYFRTNGRKGERLSSVDLERLYQNMSVWLWNEIEYSYDSSKEDELGFK
                     TFTEFLNCYANNAYTKGTQCSAELKQSLVDNKMIYGDNTAKAGMMNPSYPLNYMEKPL
                     TRLMLGRSWWDLNIKVDVEKYPGAVSAEGENVTETISLYSNPTKWFAGNMQSTGLWAP
                     AQKEVTIKSNANVPVTVTVALADDLTGREKHEVALNRPPRVTKTYDLKANGEVKFTVP
                     YGGLIYIKGNSSTDESADFTFAGVVKAPFYKDGKWKNDLNSPAPLGELESDAFVYTTP
                     KKNLNASNYSNYTDGVAEFAKELDTFASSMNDFYGRDGESGNHRMFTYKALTGHKHRF
                     ANDVQISIGDAHSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLNVPGAT
                     EVANNVLALYMQDRYLGKMNRVADDITVAPEYLDESNGQAWARGGAGDRLLMYAQLKE
                     WAEKNFDIKTWYPEGTTLPAFYSEREGMKGWNLFQLMHRKARGDDVGDKTFGGKNYCA
                     ESNGNAADTLMLCASWVAQTDLSEFFKKWNPGASAYQLPGATEISFQGGVSSSAYSTL
                     ASLKLPKPEQGPETINKVTEHKMSVE"
BASE COUNT         3701 a         4759 c         4199 g         4063 t
ORIGIN      
        1 ggtcttgaca attttggcaa aacagattta tgatggaatt caacttacta gataactgaa
       61 agttctcgag tccgggcacc actattctaa agaacccgcc cacaaggcgg gtttttgctt
      121 ttggatctga caataacctt cacgaaaaaa cttagcttat aagggctgga ggaattactc
      181 tcgccacgtt aacgagagta attttattga tattaatctc ctgatacttt accccgtcca
      241 aattccagcc gctgcacatt caccatccca ggcttctcag cagcactgac atcaatttgt
      301 gtcacccgca gcgcatattt ttcatccagt gcgtttaacc agttcagcag atcattaaat
      361 accacaggat ctatccagac ctggatattc tccccgcgct cggctattcg cttaatgacc
      421 actgaatgcg ccgaagcact gtcgctgatg acccgcgata cctgtgctgg cgttgtcgtg
      481 ccggattttc gcgccgcaat aatatccggc gcggcgctct tcagtcgcgc gttcatcgcc
      541 accagctgct gcaacatcgt ctcctgttgc tcaatccgtt cgctcaacgg ctgccagatg
      601 agaacgtaat atccggcgct aaacaggaac actaccgctg ccagtaacat gcctttttca
      661 cgcggagaac gtcccgccag gtgttgtgtc agccagtgtt cgccacggct taactggcgt
      721 tcacgccatt gctgaaaata gtgaataaat ttatcgcgta acatgttatt tcctccgcaa
      781 cgttacgccg ccggaaaccg catcaccctc tttctgtaac gcgtcctgtt gcacaacata
      841 atctgccgcc agtgcgctac gcagtttgtc gaagctggca aagttcgcag cccgcagttg
      901 gaggtgaagc gtctggcgtt tttgatcaaa ggtgaaacca cgcatctcaa tgtcggaaag
      961 tgatgcggat ttcagggtac tggcgatcgc agacaactcg gcaagcagat cggtatcatc
     1021 agcctgtggg cgatattttt tcagcgccat cgtcacctgc gagcgtaaat tcacaatccg
     1081 cttctgctcc gggaatagcg ttaagaactg tttctccgcc tgggtgcggc tttgcgccac
     1141 ctgttcgctg acgctccata acgtcacgcc ccgttccact accagcgcaa ccagaatcag
     1201 caatatcggc agaatcatca cccgccagcg cgcccactgt tttcggtagc tgacacgagg
     1261 ctgccacggc cctgttagca ggttcccttc cgattcgcca taagtggtaa tggcgggcag
     1321 agccgtaacg gtcaggcgtt cggcgtccgc accagcccat gcagacagtt cttccggtgc
     1381 aatgccgacc acagttagtg aaatcggtaa attctgctca ttgagctgtg cgcggaacat
     1441 gaccggagcc agcgcccgcc cggcgctcca tccccggcat tcatcgatgc ggcagataat
     1501 ccgttgcgca tcgccagcca taaccccaca aggaatggac atccagtccg gcgcgacggt
     1561 agcgcgggtg atgccgtttg cctgcaacca ctgcgcaatg ttgcgcatat gctgctggtg
     1621 aatcacagct acggttgcca gttgctggtc gattttcaac ggggcgaaat gcagttcatc
     1681 gatatcctgg ttcagctctt cttccagcaa ggcgggcaga atcgtcggta tctgcttgcg
     1741 gggcacatca ggcagttcaa cctgccagac gctgatccat tcgccgggaa tgtagagtcg
     1801 aatcgcatca gtttgcagcc attgctggag acgttcgtca gcaacgtcag gccagatacc
     1861 gtgctccacg tcgggtgtat ggcgctgcca acggatggga tcagcggcgc aaagcgggaa
     1921 aaaaatctca agcatggaac tcactcactt tctcctgtct gatgccagag aacagaaaag
     1981 tgttgtgggc ccatgcggac aattaacgaa ttcatcgtca gttcaatctc attcacggtg
     2041 atatctgaac gcagccagaa gtaattgctg tccacgctca ggatggtttt tagctgtttt
     2101 ttagtacgct catcgacgtc tgcaagtagc ggctgtgcaa gaaactgatc gacatcttcc
     2161 cagcccttcg ccggacgttg ttgtaataat gcccgcgcct gaacagggct taaccacggg
     2221 tcaaacagcg cctcaagaat cacactttgc gtgacatcta atgtattgat gttgatttgc
     2281 tggcgggcca tcggcagcgc acagaccaac ggtttcagtt tttgataaag cccggcgtcc
     2341 attccctgca ccacgcgcat ctcgctgata tcagccagcg gttgattagc ggcgtagaac
     2401 ggcaccgagc gggcgagata ctcgctgtct tcacgaccca gacgcgtctg cacgctgcgg
     2461 tcttcgtcaa taaactccca caggctttcg gctatcagtt cggcccgata agcaggcaca
     2521 tcgaggcgcg agatcagggc aatcagttgt tgtaccgcga gcggacgcga cgccgtcgtc
     2581 ggctgagcga gggcattcag gttaaagcag gcctgtgcgt cacgcagagt gacggcgatt
     2641 tgcccctgcg gcagtgggaa aaaacgcggg ccagaagccc acggttgtgc cagatgcaca
     2701 cgcttttcat ttttcaggct cagactgagc gcgctcagcg ccaggctttc tgcactggcg
     2761 ctgtaccaca gcgcctgctg gtactcctgc tggctgcgcg ttcgcccaag ttgttgctgc
     2821 atccgcccgg aaagcgtgat ggtcaccagc atcataaccg ccagcaatac cagcaccacg
     2881 accagtgcca ttccgcgttt tggtggtaag gtgatcatga taattgcggc ccgcgtaaca
     2941 accagatgcg ttcaatttcg ccccattgtg gcgaatgcag gattatgcgt actgccacag
     3001 gaatcgcctg cactgatgac cagctctcct gccagcgcgt gccgtcgtgg aactgcaaac
     3061 ggagcgaatc cgccgggatt aatttttgcg ttgttggctt cacgctgcct gccgcatcgg
     3121 tcagtggcca ggctaaccgt tcgagataac caccatgaat gcggtaaccg acggtgagca
     3181 gattactgcg cggcagacgc atcaacggat taaccacgcc gccacgtaca aaacgcatcc
     3241 cttcactctc agacgccagc acgccagcgc ccgccagtaa cgctggttca cgctggccct
     3301 gatcgcctct taccggacgc ggcatcattt gtgtcagatc gtggttcaaa aaactcatcg
     3361 tttgctgcat gaggtttagt ttttgatcgt gtccggcgac ggcgctattc acgcgtgtaa
     3421 cgccgtttgt cacctgctgc gccatcagtg ccagtgaggc aaaaatggct atcgccacca
     3481 gcatttccag taacgtgaaa ccagtgcgag cccttctcac tgttggcctc ccacggcgct
     3541 aaaccaggcg cgtcgtgact gaatcactgg cgaaaagtcg tcatgaagac tgacttcaat
     3601 atccaccgcc tggagcagca cgttaccggt attcagaggg atcgttcgcc agtaccagcg
     3661 gcttcctgcc atatcactct cgccctggtg cctgaccgct cgttgttcac gggtcatcag
     3721 gtaaagcagc gcagtctggt tttcagctac ccagcccgct accgttttct cctcaagaac
     3781 ctgctgattg tttagcgcgc cgctggcgat ctgtaatacc gccgtggcag ccagcgcaaa
     3841 aatcgccagt gcgatcatca cttccagcaa cgtaaatccg cgcttcatgg caccctctca
     3901 tcacattgat tgagggataa cgcgccatcg tgcgccagtt ttaccgccca acatgcgttt
     3961 tgcgccgccg aataaaatcg cagcgtaaac ggcgtggcgg gttcaaaagg cgaaaaacgg
     4021 atctgcggtg tcttcttttt cgcctctttt tgcagctcca gttcaatatc atgcagcgtc
     4081 aggcgacgac gttgcatctc cagcgcatac tccttctgcc agatatcact gccgggttgt
     4141 aacagcatct gcacctgttt cggcaccgta acctgtgccg ataaccgggt cgcagaaacg
     4201 ggcaaccatt gcccttgacg acgctgcata aactggtaac ctggcgggtc gattagcaca
     4261 ccgagtgttt gcccttcgat cactgccctg tccttaaact gcgcaaagcg agtcagaaaa
     4321 gcctgcgccg cttttttcgc aggcggctct gaagcggtcg caaaagtctg caccacgccc
     4381 gcactggcaa ggccgataag gaaaatcacc agcatgattt ccagaagtgt gaatccgcgt
     4441 tcaggcactg attactgaaa ctcctgcaaa ttccagttac cgatatctgc gccagcgccc
     4501 tccccgtttt cctgaccatc tgcccccagg gtataaacat caaacagccc tttttcacct
     4561 gggctgagat actgataatc attgccccac ggatcctttg gcagtcgttt gatgtatcca
     4621 ccggtacggt agttgcgggc gtccgccata ttggccggtt gctggatcag cgcctcaagc
     4681 ccctgctcag tggtcggata acgcccgtta tccagtcggt acatatccag cgcattctcc
     4741 agcgccacga tatcgctgat ggctttttgc cgatcggctt tctctttgtt gcccaacagg
     4801 ttaggcacca ccagacttgc caggacgcca agaataacaa tcaccaccat cacttccagc
     4861 agggtaaaac ctgcccgtgg tttttgtgtg cgggataacg aattcatgtg ataactccgt
     4921 aaattacatt ccaaccatat tgttcagttg cagcatcggc tcgaggatgg cgatgacgat
     4981 aaacagcacc acgcccgcca tcatcaccac cagcgccggc tcaaacagcc ctaacgccag
     5041 ccccacctgg gtatcaaatt cccgttcctg gttgaccgcg gcctgttcaa gcatggtttc
     5101 cagctcgccg ctctgttcgc cggaggcgat catgtacagc atcatcggcg ggaacaggcg
     5161 cagatccgcc agcgcggcgc gcaggctgct tccttcgcgg acgcgatctg ccgccagcag
     5221 cagttgttgc tcgacatagc gatttgccga cacggcggcg gcggtctgaa tgccttccag
     5281 cagcggaaca ctgctggcgg tgaggatgct taatgtgcgg gaaaaacgcg ccgtattcag
     5341 tccgcgcgca acacgccccg tcacgggcag gcgcagcaag gttttatccc agcgcaggcg
     5401 catcgcagga tttttgagta accgttgccc cagcaccaga agccccagca aacccgccag
     5461 ccagtacacg ccgctggcct gtaacgcgtc gctcatagcg atgagcattc gggtggaggc
     5521 gggtagcgcg tgtccgagat gatcaaactg ttcgataatt ttcggcacca ctgccgtcag
     5581 caaaatagtg actacgcccg ttgccaccac cagcagaacc agcggataga gcatggcctg
     5641 cagcaggcgt gatttcagac gctgccgctg ttcggtgtaa tccgccaggc gattgagcac
     5701 cacgtcgaga tgcccggatt tttctccggc agcgaccatc gaacaaaaca gggagtcaaa
     5761 gacgcgggga tgttcgcgca ggctgtccga cagggtataa ccttcctgaa tccggctgcg
     5821 cagcgccatt ccgaggcttt ttacatgcag tttttcgctt tgctcactga ccgcctgtaa
     5881 gcaggtttcc agcggcattg ctgcctgcac cagcgttgcc agttgacgag tgaacagcgc
     5941 cagatctgcc gccgccacgc gacgatgtgc gtgccgccga cgctgcaaca ttccccctgc
     6001 cgatgcattc atccgggctt caatgtgcac gggaatgagg tctttaccgc gtaacaattg
     6061 acgggcatga cgcgcggaat ccgcctcaat catgcctttg gttttgcgac cattacgctc
     6121 cagcgcctga tagtaaaaca gtgccattac gcctccatgg ttacccgcag aacttcatcg
     6181 agagaggttt ctccggcgag cactttctca atgccgttac tgcggatacc cgcagagtgt
     6241 tgccggacat aacgttccag ctccagttcc ccggcctgac ggtggatcaa atcacgcaat
     6301 gtggcgtcca ccacaatcag ttcatggatg gcagtacgtc cgcgaaaacc tttgtgatta
     6361 caggcggggc agccctgcgg atggtacagc gtgacggcat gcgcatcggt aatccccagc
     6421 aggcgttttt cttcgtcagt ggcaggcgcg gactggcagc agtcgggaca cagcgtgcga
     6481 accagtcgct gcgccatcac gcccgtcaaa ctggaagaga gcaggaaagg ctccacgccc
     6541 atatcctgca aacgcgtgat cgcccccacc gccgtgttgg tatgcagcgt ggaaagtacc
     6601 aggtgtccgg tcagtgaagc ctgaacagcg atttctgcgg tttcggtatc gcggatttca
     6661 ccgaccatca ccacatccgg gtcctgacgc aaaatcgcgc gcaggccacg ggcgaaggtc
     6721 atgccgacgc gggtgttaac ctgcgtctga ccgatccctt caatcatgta ttcgataggg
     6781 tcttcaaccg tgagaatgtt acgcgagtgg ttgttcagct cctgcaatcc agcgtacagc
     6841 gtggtgcttt tgccggaacc ggtcggcccc gtcaccagaa aaatgccgtg cggtttgtgt
     6901 aacagctggc gcaactgcgc agtcagttcg agacttaaac ccagacgctc cagcgtcagg
     6961 cgggcctggt ttttgtccag cagtcgcagc accacccgtt ctccccaggc ggaaggcatg
     7021 gtagatacac ggacgtcaat cgcccgaccg cccagcagca gcgcaatacg accatcctgc
     7081 ggtacgcgct tttcggcgat atccagtcgc gccatcacct tgatacgcga gaccagtagc
     7141 gaggccagtt tacgaccggg gcgcaacatt tcatgtaatg tgccgtcaac acgaaaacgg
     7201 atcaccagac tcttttcaaa cgtctcaatg tggatatccg aagcgccttc tttgattgcc
     7261 tctgccagca tggcgttgat cagtttgatg atcggcgcat cgtcgtcact ttccagcaga
     7321 tcttccgttt ccggcagttc ttcagcgagg gtaaaaaagt cctcggcgga accgagatct
     7381 tccatcagct ggcgagcttc ggaagagtcc cgctgccaga ccgcattcag ccgctgttca
     7441 aattcggcct cgtcgatttg ccgcagcgta aagggcgcgt tcagcccccg ttgtagctcc
     7501 tgcaaaacag agagcgacaa cggatgaaca tggaggatct ccagcgacgc ttcgcaccat
     7561 gccaccaggc taaaccgacg gctgaaactg tagggcagac gcacggtgtt agcggtggtt
     7621 tcctgtgcta caggtaccat taacgcgttc tcccggcatt gaggaacgcg cgtacttccg
     7681 gcggtaaggc ctgattttgc gctggcaata tcggctgcgc ggtgtgcggc atcaggctta
     7741 agccttgctc atcgcggtag atttgctcag cgcgcatgta gttatatttg cgctgcgaca
     7801 cgccgtctgc cgccataccg tcacgcaaaa tggtcgggcg gataaacacc atcaggttac
     7861 gtttttcttt tttatccgcc gtcgatttaa acaggttacc aatcaacggg atatcaccca
     7921 gcagcggcac tttcgccacg ctttctcccg cctggtcgtc catcagaccg ccaagcacga
     7981 tcagctcacc atcgttagcc agcacggtgg ttttcagttt gcgctcgcca aacacgacat
     8041 cgaggctggt ctgcccttcc actttcgaga cttcctgctc aatcaccatc tgtaccgcgt
     8101 taccttcgtt aatctgcggc gtgactttca gcatgatgcc gacttttttc ctctctaccg
     8161 tattgaaagg attgctgtta ttggagccaa cggtagagcc ggttaatacc ggaacgtcct
     8221 ggcccaccat gaagaaggct tcctggttgt ccagcgtggt gatactcggc gtggagagca
     8281 cgttcgagct ggagtcgttt ttgaccgcct gtaccagcgc catccagtcg cctttcacca
     8341 cgccaaccgc cgtaccgcta aagccagaaa gaagctgagc gagcgtggag agatcgccgt
     8401 tagtatccgg atttatggtg gtagcaccgt tttcgctgat caccgtggaa cctttctgcg
     8461 gcttggctgc agaaatcgct gcccccagcg taccaatagg gatttgcgta ccgttggcaa
     8521 actgcattaa tccggcatct tttgaccccc actgcacgcc gaagttgata ttgctgcctt
     8581 cggcaacttc cacgattaac gcctcgacat gcacctgagc acggcgaata tccagttgtt
     8641 caatcacgct ttgcagcgac tgcatgatgt cctgcggcgc ggtaacaatc agggcattac
     8701 tgtgtttgct ggcggcgatg gagacaacct cacgcccgct accaaccgtg ccttccgcct
     8761 cttctttagc cgccgtgagc gtaccgctga cctgcttcag cacatcgacc agatcttcgg
     8821 ctttgctgta tttgagatag aacacctggc tgttgccgct gcgctccatt tctgaatcca
     8881 gccgacggat cagacggcgc attttgtctc gcgtggcggg gtcaccactg acaatcacgc
     8941 tgttggtgcg ttcatcggcg acaatttgag atttcagcgt tgctggctgg ttctcgccgc
     9001 tgtttttggt caggctttcc agcacgcggg caatttccga ggctgaggcg ttatccagcg
     9061 ggatcacctc ttcggtgcga ttacccgcgt gatccacgcg ctggatcact tccgtcaggc
     9121 gctccacaac tgaggcgcgt ccggtaagca taatcacgtt ggagggatcg taattaacaa
     9181 cgttgcctga gcctgcgctg tcaatcatct ggcgcagaat cggtgccagc tcgcgtaccg
     9241 acacattgcg taccggcacg actttggtga ccatttcatc gcccgcgtag ttgtcgctgc
     9301 cttcaccgac cagcggcagc ggctcgactt tcgcagcgct tgatttcacc accttcagca
     9361 cgtcgttttc catcggtacg accgcatacc cctgtgcttc cagcaggtta aggaataact
     9421 ggtaatactg gcgttcattg agcggagtca tagtgcgaat actcactttc ccctgaacgc
     9481 ccggccccat aatgatggtt ttattaaggt tagcgccgac ggtttcgatg aacgatttca
     9541 ggtcggtatc tttaaaatta gcggtgaaag tggcttcttc cgcccagacc ggtgaactac
     9601 acaatgccgc tgccagcatc agcggcagta aacgcttttt tgttttgagg ccagttgtct
     9661 tcttacgcca gatagacaac gtcatatcac gccaaaacac gatgaatgat tctcctggat
     9721 taaatgcggt tagcgcagtg cgatggaaat gtcgtagcgc gcacccttgc gtaaaaccgt
     9781 aagttgaatg gaatccattg aaggtaactg ccgcatcaga gcaatcattg ctcgtggatc
     9841 agtgaaatcc tgctgattta gcgcaatggc gatatcgcct tccttaaaac cgctggcatc
     9901 gaacagagaa cgatctgccc ccggtttcac tgcataaccg acaatcccct ccttacgcac
     9961 aggcgtaagc tggatatagt taaaaatttt ctgcggatct ttcgccagtg cctgacgcac
    10021 ggcagccggg atctccactg gcgcactgac gacaggttca gcaacagctt gctttgcttc
    10081 gtcactggcg gcttttttgc tggtcaccgc cacagggggg cgttcctcct ctgccagact
    10141 cagacgttcc attttcccct gatagcgcag catcacatgg tcgcggttga tttcctcaat
    10201 cactgcgttg tgagagccaa gccgttcacc ctgcaaatag acctgctgtt tgccgccttc
    10261 ttcaataacc gcgccgggtc tggcaccaaa ggcgatcccg cgcagcacca cattaagacg
    10321 cgtttctgcc acaggcgcag attcaggttg ttttaccggt gcggcgacgg gttgatattt
    10381 gccaaaccag ttttgttggc tgactaattg cacgtcattt ttatcgaacg gttttgcatc
    10441 cgcacggagc ggtttattcg ccgatgcgga aaccgccgta tattccgcag aaaaggagaa
    10501 atagcgccag agtgaatacg ccatttttgc agaaataata agcagcatca gccagaacat
    10561 cgcgcgtgca attttgcgca gatgctcttt gtctgtatta agagattgtc tctggctaag
    10621 agtgtgacga attttagtca gccattgaat gagataaatt cgtgcgtcac gaaaaacaac
    10681 ccgcgccaat ttatatctac ccgacgttat gctttgacta ttccacaggt ggtacgatcc
    10741 agtttccgct gatactggtt acctgtacgt gtatcattaa tcgttatgct gtaattaatt
    10801 ccctcggtga tcattaattt caccgttggg tcagcgcaca tttgtcgttg atagctggtt
    10861 aaaaaggcgt caggcgtctg cgtggtctga gtacccgatt cgctgataat ggtcattttt
    10921 accgtcgtgc cactactttg cgccagcact aaggtatatc ccgcagattt aatcggcagg
    10981 ttttggctga tattttgcgc ctgttttttc gccagtaaac tggcgttttc attatggctg
    11041 gcgcagccac ttaataatcc tgtaacgctc aacaatagcg atattaatac cctccctggc
    11101 atctgtttta tcgacatggt aaataatctc taaggttatt aataagagtt aaaatgtcac
    11161 tttgataatg acgtggttat cattaaaaca atgcctgtag ataaagtgtt gctataccgc
    11221 ccagacttaa acacggtcca aaaggcagtg tggttgatcc tctttttgta ataacggcat
    11281 atatcagacc gcagcatgat gcgattaaag ccacattggg tagcgacaac ggccccaccc
    11341 agccacctaa agcggcgaag agtaatacat cgcccatgcc taatgcttct ttacgcagaa
    11401 ctattccggc tatccagcgc agggagtaaa aagtgataaa ccccaccaga acgccggtga
    11461 ctgcgtcttg tagcgttaac ggactctgct gcgcccatgc cgcaatcagt ccggtccaca
    11521 atacgccctg agtaaaaaca tcgggcagcc attggttgtc gaggtcgatg atgctcgcgg
    11581 caatcagcca ggcggataat atcatcaccg ccagccccca tccactttct ggccacacca
    11641 gactcgccag caaaaaagcg agtgctgtca ataactcaac cagcggataa cgcttgctga
    11701 ttttcgcctg acagtcgcgg cagcgccctt tgagcatcaa ccatgagagc agcggaatat
    11761 tgtcacgaac gcggatagtc tgctggcaat gcggacagtg cgaacgcggt agcgcaaggc
    11821 ttatttttga ctgcgcactc ggcatttcac catgaaactc cgccatttgt tggcgcagca
    11881 tgatggggta acgccaaatc accacattca aaaaactgcc gatgattaat cctccgacgg
    11941 ttgccagtat gggcatcgcc gcggggtatt gctgaaaaac atcaaaaagc atggttaaag
    12001 gttgtttgtt gtaacttgcc ggatgcgggc ctgcgggtgt atgccatatg gctttcctcc
    12061 aggcccgata agctggcgca acccggtgcg ccttatttca tgccggatgc ggcgtgaacc
    12121 cttatccggc atacaggatt actcgacaga catcttatgc tcggtaacct tgttaatggt
    12181 ttccggtccc tgttccggtt tcggcagctt gagtgacgcg agcgtgctgt aagccgaaga
    12241 gctcacaccg ccctggaaac taatctccgt tgctcccggc aactggtaag cacttgcacc
    12301 cggattccat ttcttaaaga actccgaaag atccgtctgg gcgacccagg aggcacacag
    12361 catcagcgtg tccgccgcgt taccgttgga ttcggcgcag taattcttgc caccaaaagt
    12421 cttgtcgcca acatcatcgc cgcgtgcttt acgatgcatc aactggaaca ggttccagcc
    12481 tttcatccct tcacgctcgc tgtagaacgc aggcagggtt gtaccttctg gataccacgt
    12541 cttgatatca aagtttttct ctgcccactc cttcaactgt gcgtacatca gcagacggtc
    12601 acccgcaccg ccgcgcgccc aggcctgacc gttgctctcg tccagatatt ccggcgcgac
    12661 ggtaatgtcg tcagcgacac ggttcatctt accgagatag cgatcctgca tgtacagcgc
    12721 cagcacgttg ttcgccactt cagttgcacc cggtacgttc agcggtgttt ctgcagcgtt
    12781 atgaccgact tcgtgccaga tcaaccagtc gttcagcggc gtcgtcggca gcgtggtgct
    12841 gttcgtcgag aagctgctgt tcattaccgg ataacccgag tgcgcatcac cgatggagat
    12901 ctgcacatcg ttggcgaaac gatgcttgtg acctgtcaat gccttatagg taaacatccg
    12961 gtggttacca ctttcgccat cacgaccgta gaagtcattc atcgagctgg caaaggtatc
    13021 cagttcttta gcgaattctg ctacgccgtc agtgtaatta ctgtaattgc tggcgttcag
    13081 gttcttcttc ggcgtggtat agacaaaagc atctgactcc agctcgccca gcggggccgg
    13141 tgagttcaga tcatttttcc atttaccatc tttatagaac ggcgctttta ctacaccagc
    13201 aaaggtaaag tctgctgatt catccgttga gctgttgccc ttgatataaa tcagaccacc
    13261 gtaaggaacc gtaaacttca cctcaccatt ggctttcaga tcataggttt tagtcactct
    13321 tggcggacgg ttcagcgcaa cttcatgctt ctcacgtccg gtcaggtcgt cagccagcgc
    13381 cacggtgaca gtcacaggaa cgtttgcatt ggacttaatg gtgacctctt tctgagccgg
    13441 agcccacaga ccagtggact gcatgttacc cgcaaaccat ttggtcggat tcgagtacag
    13501 gctgatggtt tcagtaacat tctcaccttc agccgatact gctcccggat acttctcgac
    13561 atcaactttg atgttcagat cccaccagga acgccccagc atcagacgcg tcagcggttt
    13621 ttccatatag ttgagcggat agctcgggtt catcatgccc gctttggcag tattatctcc
    13681 gtagatcatc ttgttatcga caagcgattg tttcagctct gcggagcact gcgtgccttt
    13741 ggtgtaagca ttgttggcgt agcagttcag gaactcggtg aacgttttaa agcccagctc
    13801 gtcctccttg ctgctgtcat agctgtattc aatttcattc cacagccaga ccgacatgtt
    13861 ctggtacaga cgttccagat cgacgctgct cagacgctca cctttgcgac cattggtacg
    13921 gaaataaagc tcgtgctgat acaggcgctg aatgttggtg cctaaatctg ccgcctgcac
    13981 catcgctttc gcggtgtcgg cgctgagatt cagttgcgta tagcgcggaa catacatgcc
    14041 tccggttacc ggaacgttcg tgccaggacg atgttccagg cagttgacct cgtagtgata
    14101 tgtcgaatcc ttacactcct gtaatccagg aaacttctca aagatttttg cctttgcagc
    14161 atccagagat tcttttgttg tgtattccgc ttcatcaata aaggcataac gcgttacctg
    14221 tttgccctca acttcctcct gccagctcgc aacttccagt ttcggcttgt catcaggctt
    14281 gttgtcttgc tggtatttcc agataacttc tttcgttgtt tcatcaatgg tatacggcgg
    14341 ttttccatca acaaacggat aacgttcata tacccagata cctttttcgc gctgctgacg
    14401 aacgcgatcc ggataccctt gcggatcgtt gttcaccacc gatttgttca gagccattga
    14461 cagacccgcg gcatccagca gacgcacaaa actggacgcg ctctcttcct taagattgct
    14521 catcacgttt tccatgatca gcaccgagcc acctttgttc agataagcga tcagatcggt
    14581 cacatcctgc tgagtcagct tcggtttgct ggtatctgct cgcagaggaa tagagtaagg
    14641 atcgctaccc cattgtgtga cgtattcaaa gccattgagg atcaacagag gaacttcatc
    14701 tggattcaga ttgccatagc tggtcatcgg tttgaccgtg atgccagcga agtccttgtg
    14761 gaaagcaaac ggtgcactat ttcctaatac ctgacccgct tttttgaaat aaaccgtatc
    14821 caggttagtg cccacggtca tactgctctt cgcatccggc agccagcgtt catttgacag
    14881 gtagcgcaac acgttctcca tgaagttctt catgtcgtcc gggtcgctgt tgagcgtaca
    14941 ctgcccgtct ttattaacgc ccccgttcca gctgtaaccg ttcggacaac gcaaaatgct
    15001 gttgtagtgc gggttaccga taaccatcag tttgccctca ccgacttgcc ccagcgaaat
    15061 aaacggcagg ttaaaggtgg cggtatcgcg cgtaacgttt tccggctcaa caagagaagg
    15121 cgcttccgta atgtacgcca gctcgttttt atcccaggcg cgtttttcgc caaacgccag
    15181 ccagtagttt ttatcattac gcgccatcag aatcgggaat gccgcgttgg agatatttac
    15241 caccgcctga ccgcgcgcgt taccggtact gccatagaag ttggtggagt catggaagac
    15301 gtggaatttc gtcactgatt tgtaatcttt atccacaccc cacagcttgt tgataacgcc
    15361 ctgaatctgg ccgtcattaa cattgcgcgt tgtcagcgag aaccagcgag cctcgttaca
    15421 accgtcggtt ttcgcacaaa tcgcggtatc gatctctttg gcctgacccg tcttaaactg
    15481 ctcgataaat tcgttaggca gcacaacgtt ttgatcgcct tcatccagcg tcgcaccatt
    15541 ggataacgag agattgataa tctcgttgat cacgttggga tattcggcaa agaccttgcg
    15601 tacatcgtcc ggaacaacac gggtatcatt ttttccggcc gtcgaatagc ggtgaatcag
    15661 ctgatcgata tttgctccgc gaacttcatc gcccagttca gtcagcgcaa tggtcgactt
    15721 attaccgcgc accgagccca gttcgaaggt gtcgataccg aaagaaatgg tttcgcccca
    15781 gctaaaagag aaattaccgt tttcgtcagt tacaccgcga ccagagctgg tgtaatagtt
    15841 aacaccagca acaccatacc cctggctatc taccagtcga ccttcagaaa gaatgatttc
    15901 cgtaggttga tactgataaa actgttccgc attagctgac acgaagctag cgttgagatc
    15961 cggcgatgtt ccctctgtcg tagctggcac gaccgatgag gtatgggtgg atggcgcttt
    16021 atcagtcgca gcattgtttt ccacctcttc attgaccagc tttttgaact cgtccggtgt
    16081 cagaccgatt tgcttataca gtgagtcaaa acgtttactt tcggtcaccg aggagaacgt
    16141 caggcaaacc tgttctgtat ccgctggaca actgttactg gacgttacca gtgagacggc
    16201 attggctttc ttgtcgttgg actccgccag ttctgtcgcg tcctcaagac taaacgacac
    16261 tttttcaacc gcacgcaggc tacgcgcagc ttctgactgg gtgtcgaagg tagcaattgt
    16321 cgtgttgttc cctgccacac aggtgacttt gtcgcctggc gtaaaggtaa agccatcgct
    16381 ggattcacca ttacaagtag caccagttat ccgctggctt ccgcccagag tcagatagcc
    16441 cgttttcgtg ggaacaggtt ctggctccgg ttctggtgtc ggctctggat cgggtgtcgg
    16501 gtccggtgta ggttctgggt ccggcgtcgg ctccgggttt ggtgttggat caggtttcac
    16561 atccggcaaa gaccctgaac cagaatctgc atgtggcgta tcggaggatg accccgaacc
    16621 accgccatca caaccggcta acagggttgc gcttaaaata gccgctaaaa gcgatttctt
    16681 atatttaaat ttcttattca ttaataacgc aagtgacaaa ac
//
LOCUS       DAZFJL010000103        16648 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFJL010000103 DAZFJL010000000
VERSION     DAZFJL010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.103"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..385
                     /locus_tag="ACHJ8C_003335"
     CDS             <1..385
                     /locus_tag="ACHJ8C_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO5553138.1"
                     /translation="ALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINILFTSRQM
                     IIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALSLKSSLE
                     DILLEITSLVDNALDLAEITEEYSH"
     gene            404..1102
                     /locus_tag="ACHJ8C_003336"
     CDS             404..1102
                     /locus_tag="ACHJ8C_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO5553139.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEENQGVRMLEQHIRDAKTELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            1152..1811
                     /locus_tag="ACHJ8C_003337"
     CDS             1152..1811
                     /locus_tag="ACHJ8C_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO5553140.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDAL
                     LIALPGEEYTVAAVSCIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            1829..2227
                     /locus_tag="ACHJ8C_003338"
     CDS             1829..2227
                     /locus_tag="ACHJ8C_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO5553141.1"
                     /translation="MHILDSLLVFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAAALAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            2237..2875
                     /locus_tag="ACHJ8C_003339"
     CDS             2237..2875
                     /locus_tag="ACHJ8C_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO5553142.1"
                     /translation="MARKRKSRNNKNIGHGAISRIGRPHNPFEPRRNRYAQKYLTLAL
                     MSGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNSDICAHGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            2878..4041
                     /locus_tag="ACHJ8C_003340"
     CDS             2878..4041
                     /locus_tag="ACHJ8C_003340"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO5553143.1"
                     /translation="MLRHNVPVRRDLDRIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGVIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEELGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFDGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            4125..5750
                     /locus_tag="ACHJ8C_003341"
     CDS             4125..5750
                     /locus_tag="ACHJ8C_003341"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO5553144.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGASWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLDDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHILSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGIPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCKVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5867..6142)
                     /gene="yjfN"
                     /locus_tag="ACHJ8C_003342"
     CDS             complement(5867..6142)
                     /gene="yjfN"
                     /locus_tag="ACHJ8C_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO5553145.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(6291..6620)
                     /gene="bsmA"
                     /locus_tag="ACHJ8C_003343"
     CDS             complement(6291..6620)
                     /gene="bsmA"
                     /locus_tag="ACHJ8C_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO5553146.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            6802..7551
                     /gene="yjfP"
                     /locus_tag="ACHJ8C_003344"
     CDS             6802..7551
                     /gene="yjfP"
                     /locus_tag="ACHJ8C_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5553147.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLRDDRLAVGGASMGAMTALGITARHPTIKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFLQHL"
     gene            complement(7548..8303)
                     /gene="ulaR"
                     /locus_tag="ACHJ8C_003345"
     CDS             complement(7548..8303)
                     /gene="ulaR"
                     /locus_tag="ACHJ8C_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO5553148.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(8411..9475)
                     /gene="ulaG"
                     /locus_tag="ACHJ8C_003346"
     CDS             complement(8411..9475)
                     /gene="ulaG"
                     /locus_tag="ACHJ8C_003346"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO5553149.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGANVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GTLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADILRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9830..11227
                     /gene="ulaA"
                     /locus_tag="ACHJ8C_003347"
     CDS             9830..11227
                     /gene="ulaA"
                     /locus_tag="ACHJ8C_003347"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO5553150.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIERMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            11243..11548
                     /gene="ulaB"
                     /locus_tag="ACHJ8C_003348"
     CDS             11243..11548
                     /gene="ulaB"
                     /locus_tag="ACHJ8C_003348"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO5553151.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            11558..12022
                     /gene="ulaC"
                     /locus_tag="ACHJ8C_003349"
     CDS             11558..12022
                     /gene="ulaC"
                     /locus_tag="ACHJ8C_003349"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO5553152.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            12036..12686
                     /gene="ulaD"
                     /locus_tag="ACHJ8C_003350"
     CDS             12036..12686
                     /gene="ulaD"
                     /locus_tag="ACHJ8C_003350"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO5553153.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIGQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            12696..13550
                     /gene="ulaE"
                     /locus_tag="ACHJ8C_003351"
     CDS             12696..13550
                     /gene="ulaE"
                     /locus_tag="ACHJ8C_003351"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO5553154.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            13550..14236
                     /gene="ulaF"
                     /locus_tag="ACHJ8C_003352"
     CDS             13550..14236
                     /gene="ulaF"
                     /locus_tag="ACHJ8C_003352"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO5553155.1"
                     /translation="MQKLKQQVFEANMDLPHYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(14365..14640)
                     /gene="yjfY"
                     /locus_tag="ACHJ8C_003353"
     CDS             complement(14365..14640)
                     /gene="yjfY"
                     /locus_tag="ACHJ8C_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO5553156.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            14968..15363
                     /gene="rpsF"
                     /locus_tag="ACHJ8C_003354"
     CDS             14968..15363
                     /gene="rpsF"
                     /locus_tag="ACHJ8C_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO5553157.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            15370..15684
                     /gene="priB"
                     /locus_tag="ACHJ8C_003355"
     CDS             15370..15684
                     /gene="priB"
                     /locus_tag="ACHJ8C_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO5553158.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            15689..15916
                     /gene="rpsR"
                     /locus_tag="ACHJ8C_003356"
     CDS             15689..15916
                     /gene="rpsR"
                     /locus_tag="ACHJ8C_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO5553159.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            15958..16407
                     /gene="rplI"
                     /locus_tag="ACHJ8C_003357"
     CDS             15958..16407
                     /gene="rplI"
                     /locus_tag="ACHJ8C_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO5553160.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
BASE COUNT         3991 a         4007 c         4451 g         4199 t
ORIGIN      
        1 ggcgctggcg acggcgctgc aaactgtacc tgaacaaaat attgatgtaa caaatagcga
       61 aagcgcatta attattaaaa tgaatgatta tggcgatttg caaatcaata ttctttttac
      121 ttcccgccaa atgatcatcg aaacctttat ttgtccggta agtagtatca gcaatccaga
      181 tgaatttaat accttcttat taagaaatca gaagatgatg ccgttgtcat cggtagggat
      241 ctccagcgtg caacaggaag agtattacat tgttttcggt gcgttatcgc ttaaatcttc
      301 tcttgaagat atcctgcttg agataacttc gctggtagat aacgcattgg atctggctga
      361 aattacagaa gaatattcac actaattaca aggacgggta tttatgggga ttttaaaaag
      421 tttatttacg ctggggaaat cgtttatctc ccaggcggaa gagtccattg aagaaaacca
      481 gggcgtgcgc atgctggaac agcatattcg tgacgcaaaa actgaactcg ataaagccgg
      541 aaaatctcgc gttgatctgc tggcgcgagt gaaattaagt cacgataagc tgaaagattt
      601 acgtgagcgc aaagccagtc tggaagctcg tgcgctggaa gcgttaagca agaacgtgaa
      661 tccgtcgttg attaacgaag ttgctgaaga aatcgcgcgc cttgagaatc tcattaccgc
      721 tgaagagcaa gtgttgtcga atctggaagt ctctcgtgat ggcgtggaaa aagcagttac
      781 agcgacagcg cagcgtatcg ctcagtttga gcagcaaatg gaagtcgtta aagccactga
      841 agccatgcag cgtgcacaac aggctgtaac gacctctact gttggcgcat cttccagcgt
      901 ttcgacagcg gcagaatcct taaaacgcct gcaaacgcgt caggccgaac gtcaggctcg
      961 cctggatgct gccgcacagt tggaaaaagt cgcagacggt cgcgatctcg atgaaaagct
     1021 ggcggaagcc ggaattggcg gtagcaataa aagtagcgcc caggatgtat tagcaagact
     1081 gcaacgccaa cagggcgagt aattttttgc cagcctcact gcaaggctgg caactatttt
     1141 aaggataaaa tatgtctggt tttttccagc gtctgtttgg caaggataat aagcctgcta
     1201 tcgctcgcgg tccgttgggt ctgcatctca atagtggttt tacgctcgat acgttagcgt
     1261 ttcgtttgct ggaagatgca ttgctgattg cgctgccggg tgaggaatat accgtggccg
     1321 ccgtcagttg catcgatctg ggcgggggta gccagatttt ccgttactac acttcgggcg
     1381 atgagttcct gcaaatcaat accactggcg gcgaagatat tgatgacatt gatgatatca
     1441 agctctttgt ctatgaagag agctacggta tcagtaaaga gagtcactgg cgcgaggcga
     1501 tcaacgccaa agtgatgggg gcaatgacct taaactggca ggaaaaacgc tggcagcgat
     1561 tttttaacag cgaagaaccg ggaaatatcg aacccgtcta catgctggaa aaagtagaaa
     1621 atcaaaacca tgccaaatgg gaagtgcata attttaccat gggctatcaa cggcaagtga
     1681 ccgaagatac ttacgaatat ctgctgttaa atggtgagga atcttttaat gatctcggcg
     1741 agccggagtg gttattttcg cgcgcgctgg gggtcgatat cccgctgaca tcacttcata
     1801 ttattggtta attacaagga cgtttacgat gcatatactg gattcacttc ttgtctttag
     1861 cgcttatttt tttattggcg tggcgatggt gattattttc ctgtttatct actccaaaat
     1921 tacaccgcac aacgaatggc agttaatcaa aaacaataac accgcggcgg cactggcgtt
     1981 cagcggaaca ttgttggggt acgtgatccc cttatccagt gcggcaatca atgcggtgag
     2041 tattccagac tatttcgcat ggggcgggat tgctctggtg attcaattac tcgtttttgc
     2101 tggcgtcagg ctttatatgc ccgcattaag cgaaaaaatt attaatcaca ataccgcagc
     2161 aggaatgttt atgggaaccg ccgcgctggc tggcggtatt tttaacgcag cttgtatgac
     2221 atggtaatgg acgatcatgg ccagaaaacg caaatccaga aataacaaaa atatcggaca
     2281 tggggcaatc agtcgcattg gtagaccgca taatcctttt gagccgcgcc gcaatcgcta
     2341 cgcacaaaaa tacttaacgt tggcactaat gagcggtgcc gcttttttcg tattgaaagg
     2401 ttgtggcgat agtggcgatg tcgataacga cggcgacgga acgttttacg cgactgtgca
     2461 ggattgtatt gatgatggta ataattcaga tatctgtgct catggctgga acaacgccaa
     2521 agcggcgttt tatgccgatg ttccgaagaa tatgactcag cagaattgtc agtctaagta
     2581 cgaaaattgc tactacgaca atgttgaaca gagttggatc ccggtcgttt ctggattttt
     2641 gttaagccgg gttattcgta aagatcgcga tgagccgttc gtctataaca gcggcggatc
     2701 ctcatttgct tcccgccccg tctggcgcaa cacttccggt gattactcct ggcgctccgg
     2761 ttctggcaaa aaagagtctt actcttcggg cggctttacc accaaaaaag cgtcaaccgt
     2821 ttctcgcggc ggctatggtc gttcttccag cgcccgtggg cattggggag gctaatcatg
     2881 ctgagacaca acgttcctgt gcggcgggat ctggaccgga ttgccgccga taacggattt
     2941 gactttcata tcatcgacaa tgaaatctat tgggatgaga gtcgggctta ccgctttact
     3001 ctgcgccaga ttgaagagca gatcgaaaaa ccgactgcgg aactgcatca gatgtgcctt
     3061 gaggtggtgg atcgcgcggt taaagatgaa gagatcctga cgcaactggc gatcccgccg
     3121 ttgtactggg atgtgatcgc cgaaagctgg cgcgcacgcg atccttcgtt gtatggccgg
     3181 atggattttg cctggtgtgg taatgcaccg gttaagttgc tggagtacaa cgccgatacg
     3241 ccaacttcat tgtacgagtc ggcttatttc cagtggttgt ggctggaaga tgcccggcgc
     3301 agcggcgtta ttccgcgtga tgccgatcag tacaatgcca ttcaggaacg cctgatttca
     3361 cgttttagcg agctttacag tcgggaaccg ttttattttt gctgctgtca ggacaccgac
     3421 gaagacagga gtaccgtgct gtacttgcag gactgcgccc agcaggcagg gcaggagtct
     3481 cggtttatct acattgaaga gctcggtttg ggcgtcggcg gcgtactgac cgatcttgat
     3541 gataatgtca tccagcgtgc atttaagctg tatccgctgg agtggatgat gcgtgacgat
     3601 aacggtccgc tgctgcgcaa gcgtcgtgag caatgggtgg agccgttatg gaaaagtatc
     3661 ttgagtaata aagggctaat gccgctgctt tggcgcttct tccctggtca tcctaacctt
     3721 cttgcatcct ggttcgatgg tgaaaaaccg cagattgccg ctggcgaaag ctatgtgcgt
     3781 aaaccgattt actcgcgcga aggcggaaac gtcaccattt tcgacggtca gaataacgtt
     3841 gttgaccacg ctgatggtga ttacgccgat gaaccgatga tctaccaggc gtttcaacct
     3901 ctgccgcgat ttggcgatag ctacacactc atcggtagct ggattgtcga tgatgaagcg
     3961 tgcggaatgg ggatccgtga agataacacg ctgatcacca aagacacctc acgtttcgtt
     4021 ccgcattaca ttgctggata agaatgtttt agcaatctct ttctgtcatg aatccatggc
     4081 agtgaccata ctaatggtga ctgccattga tggagggaga cacagtgcac tggcaaactc
     4141 acaccgtttt taatcaacct ataccattaa ataacagcaa cttatacctg tctgatggcg
     4201 cgctttgcga agcggtaacg cgtgaaggtg ctagctggga tagcgatttt ctcgccagta
     4261 ttggtcagca gttaggaacg gctgaatccc ttgaactggg gcggctggcg aatgtgaatc
     4321 cgcctgaatt attgcgctac gatgcgcaag gacgccgtct ggacgatgtg cgttttcacc
     4381 ccgcctggca cctgctgatg caggcgctat gtaccaatcg ggtgcacaat cttgcctggg
     4441 aagaagacgc tcgctccggc gcatttgtgg cgcgcgcggc gcgttttatg ttacatgcgc
     4501 aggttgaggc tgggtcgtta tgtccgataa cgatgacctt tgccgccacg ccattgttgt
     4561 tacagatgtt acccgcgccg tttcaggact ggaccacgcc gctgctgagc gatcgctacg
     4621 attctcactt attgccaggt gggcaaaaac gtggcttgtt gatcggcatg ggaatgacgg
     4681 aaaagcaggg cggttccgat gtcatgagca acaccacccg cgcagagcgt ctggacgatg
     4741 gttcttatcg gctggtgggg cataaatggt ttttctcggt gccgcaaagc gatgcgcatc
     4801 tggtgttggc acagaccgcg ggcggtttgt cctgcttttt tgtgccgcgc tttttgcctg
     4861 acgggcaacg caacgcgatt cgcctcgagc ggctgaaaga taagctgggt aatcgctcta
     4921 acgccagctg cgaagtggag tttcaggatg ccattggctg gttgttgggg caggaagggg
     4981 aaggaattcg tctgatcctg aaaatgggtg ggatgacgcg ttttgattgt gccctgggta
     5041 gccatgccat gatgcgccgt gcattttcgc tggcgattta ccatgcacat caacgccatg
     5101 tttttggtaa tccattgatc caacagcctc ttatgcgtca tatcttaagt cgcatggcac
     5161 ttcagcttga agggcaaacg gcgttgctgt ttcgtcttgc gcgagcgtgg gaccggcgcg
     5221 ccgatgccaa agaagctctg tgggcgcgtt tatttacgcc tgcggcgaaa tttgtgatct
     5281 gcaaacgtgg cattccgttt gtggccgaag cgatggaggt gctgggcggc attggttatt
     5341 gcgaggagag cgagctgccg cggctttacc gggagatgcc ggtaaacagt atttgggaag
     5401 gttccggcaa tattatgtgc ctggatgtgc tgcgcgttct caataagcaa gcgggcgtat
     5461 acgacttatt gtcggaagca tttgtggaag tgaaagggca ggatcgctat tttgatcgcg
     5521 cggttcgtcg tttacagcag cagctgcgta agccagctga agaattgggg cgagagatta
     5581 ctcatcagct attcctgctg ggctgcggtg cgcaaatgtt gaaatatgcc tctccgccaa
     5641 tggcgcaggc gtggtgtaag gtgatgttag atacgcgcgg cggcgtacgg ttgtcagagc
     5701 agatccagaa tgatttattg ctgcgggcga cgggaggagt gtgtttgtaa gcgtatacga
     5761 ctgatgcgac gctggtttcg attaactaaa tgaaatattt taaaattgta ggccggacaa
     5821 ggcgctcgcg ccgcatccgg cattgttcat ttgctgtaga tgacactcac gcatacagta
     5881 tcgcctgtac gcgccagtta tcaggctgct ggtcttcgta catggtaata atgcggtacc
     5941 atgacgcacc ttgttcatcg gcctttaacg ccacgatacg ttccacgtcc tgcggatccc
     6001 ctgaaatatt gctaactgag atctgaccta tttcattcag gcccgtcacg cagtcagcac
     6061 tggcgaattc tgcagactgt gccgtggcgc tcgcaggata agtcactaat tgcagcgagg
     6121 gtgaggcaag taattgtttc atcgtcagct ccatttttcg tttccccaca tgttgcaggg
     6181 cattccatcg cggtaataaa cgcccgagct tccagccggt aatttattgt gcacaggtaa
     6241 acgcataggg acgcaaagca atgtaaagct gactatgtaa gttgtctgat ttatttacgg
     6301 tacaaaatgg cttgtgaata ccactgtcct gtcacgatgg tttcgtccac catgacgaca
     6361 acgtaataat cagcttttgc agcgacagct ttcgctctga tttctgctaa tgcgtcatcc
     6421 ggagaacccc gaaccatcgt gcttacgcta cctattcgct gtaacccttg cgtctggtcg
     6481 cggcgaatct cttgcggatg gtccgttact ggcggtgctg gctgtggcgt accttgcagt
     6541 gcgctacagg cacttaacat cagcaccaat aataaactgg caaaccggta aataacgcta
     6601 ttacgtttcc tgctaaccat agtgtagtgc cttattaatt taactttggg ggaatgttcc
     6661 cgaattgtta cctggtacgc gattttcgaa tgaaaacgtg acgaaagcgt aacccacatc
     6721 tcaacattat gaactaatac cgccagcaga aacttgtgct aaggatttct tcatgtcata
     6781 ccagacaaaa aggagagaca gatgattgaa atagaatcac gcgagctggc agatattccc
     6841 gttcttcatg cttatcctgt cgggcaaaaa gataccccgt taccgtgtgt gattttttat
     6901 cacggcttta cttcgtccag tctggtgtat agctattttg ccgttgcgct ggcgcaggct
     6961 ggtttgcggg tgatcatgcc ggatgcgccc gatcacggta gccgttttag tggcgacgca
     7021 gcgcggcggt taaatcaatt ctggcaaatc ttgctacaaa gtatgcagga attcactacc
     7081 ttacgtgcgg caatagccga agaaaactgg ctgcgtgatg accgtctggc agtcggtggc
     7141 gcgtcgatgg gcgcgatgac ggcactggga attaccgctc gccaccccac gataaaatgt
     7201 accgccagca tgatgggatc gggctatttt acatcgctcg cccgttcact gtttccaccg
     7261 ctgatacctg aaacggcagc acagcagaat gaattcaata acattgtcgc gccgctggca
     7321 gagtgggaag cgacaaacca cctggaacaa cttggtgaca gacctctact gctgtggcat
     7381 ggccttgacg atgacgtcgt gcctgccgac gaatcactac gtttgcagca ggccttaagc
     7441 gagacggggc gggataaact gctaacctgt tcatggcagc caggcgtgcg tcaccgcatt
     7501 acgcctgagg cgttagatgc tgccgtgaca tttttcctcc agcatcttta aacacgcaga
     7561 atgctgaccc cttgcgcttc cagctgttgc aggatttccg ggttagcatt tttgccggtg
     7621 atcagcatat cgatttgatc ggcacggcta aaaagcattc ccgcgcgttc gccaatctta
     7681 ctgctatcaa ccagtaccac cagtttccct accacgctca gcatcttctg ctctgccatt
     7741 gccgtcagca tatcggtttt atacaaccct tctgctgtca gtcctttgcc gctggtaaac
     7801 atccagtgcc cggcatagag actgttttcg ctgccttgcg ggcttaaagt gatggactga
     7861 cttttgttgt actgtccgcc cataatgatc acgctgtcat gctcctgatc gatcaggtaa
     7921 tttgccagcg gtagataatt agtgatgatt tgcactggct tgccacacat ttcccgccca
     7981 agcagaaacg cggtggagcc gcagttgatg actacgcttt cgcccggatt aaccagctgc
     8041 gaggccgctt tagcaatacg tactttttca tcgtgattct gcgcctgatg cagattcatc
     8101 ggcgtccagc gcgggcgctg ttgggtaata gcttctgcgc cattgcgcac ttttttcagt
     8161 ttgccgcttt cgtcaagttt attgatatcg cgtcgtgcag tggcaggcga aattcccaga
     8221 cgctcaacga ctttctcaac ggtcacaaag cccaattgtg cgagcatttc caggaggatt
     8281 tgatgtcttt gtgcttcagt catgagctat tccgataaaa tttgattttt ttagatgata
     8341 tttgaaatag ccaggaaata ctatgccgga tagcgcgagc atctccacgc tatccggtac
     8401 aagcgacaga ttacaggaac gacttgaacg gcagatctgg ttcaatagtg aagcaatcat
     8461 cgaaaccacg cggatagtgg tactcgaagt tgtctttatc cagcggccag gtaaatttgc
     8521 cgccgacctg ccagataaac ggcttgaagc catacttcag gcgatctttt ttcatctccc
     8581 acagcacgcg gatctcttgc ggatcggcct ggaagtttga ccagatatcg tggtggaacg
     8641 ggatcactac tttcgcattc agcgcttcgc ccatacgcaa gatatcagca ctggtcattt
     8701 tgtcggtaat accgcgtggg ttttcgccgt atgaacctaa agccacgtcg atctgatgct
     8761 cattaccatg ttttgcatag tagttagagt agtgggaatc gccgctgtga tacagggtac
     8821 cgcctggggt tttgaacaga tagttcaccg cgcgatcgtc catgccgtcc ggcagtacgc
     8881 cagctgcttt ttgatcggca ggcagggtga tcagtgccgt gcggtcgaaa gcgtcaagtg
     8941 catgaatttc aatgtctttt accttcacta cgtcgcccgg tttaacgacg atgcaacgct
     9001 ctttcggtac gccccagcca atccacaaat caacgcaggt tttcggtcca atgaacggta
     9061 cgtcatcagc acagttctgc atcacggcag cagcgacgtt aacgtcgata tgatcgttgt
     9121 gatcgtgagt cgccagtacc gcgtcgatct ggcgaatcgc aaacggatca agaacaaacg
     9181 gggtggtgcg caggtttggc tgcagttttt tcacgccagc catgcgctgc atctggtgac
     9241 cctgtttcat taacgggtta ccgtggcttt gtttgccagt accgcaccaa aaatcaacac
     9301 aaacgttggc accgccttct gatttcaacc agatcccggt acagccgagc caccacattg
     9361 cgaaagtgcc aggtgcgacc tgctcttgct caatttcttc attcagccag ctaccccact
     9421 ccgggaaagt gctcaggatc caggactcac gggtgatact tttcacttta ctcatcgcca
     9481 tttaccttcg tgatagttca attcgaatca atatgtgatt ggttttgatt aatcctggca
     9541 ctattttttc aggaaggcaa tgaccatttt ttgacttttt agcaggagag ttattgtgga
     9601 ttttgagtgt ggaaagatta aattgaatgt gtaatgcaat taactaaatg aatttaaatg
     9661 aataaatatt tcattatgtg aatcccattc tgtttatatg gaattatttg cgtgcgacgt
     9721 cacatttaat cataaataat cttgttgtga ttacttttga aaattagagt gagagcacaa
     9781 cattccgggt gtacgaaata cccggttacc tcttcttcag gagatcgtta tggagatcct
     9841 ctacaacatc tttaccgtgt tttttaacca ggtcatgacc aatgccccgt tgttgctggg
     9901 tattgtgacc tgtctgggtt acatcctact gcgcaaaagt gtcagcgtta ttattaaagg
     9961 tacgattaaa accataattg gtttcatgtt gttgcaggca gggtccggca tcctgaccag
    10021 caccttcaaa ccggtggtgg cgaaaatgtc cgaagtctac ggcattaacg gcgcaatttc
    10081 cgatacttac gcgtcgatga tggcaaccat tgaacgcatg ggcgatgcct atagctgggt
    10141 gggttacgcg gtgttgttag cgctggcgct gaacatctgt tacgtgctgc tgcgccgaat
    10201 taccggtatt cgcaccatca tgctgactgg tcacatcatg ttccagcagg cggggctgat
    10261 tgccgttacg ctgtttatct tcggctactc catgtggacg accattatct gcaccgcgat
    10321 tctggtttcg ctctactggg gcattacctc caacatgatg tacaagccga cacaggaagt
    10381 caccgacggc tgtggtttct ccattggtca ccagcaacag tttgcatcat ggattgccta
    10441 taaagttgcg ccgttcctcg gcaaaaaaga ggaaagcgtt gaagacctca aactgccagg
    10501 ctggctgaat attttccacg acaacatcgt ctccacagcg attgtcatga ctatcttctt
    10561 tggtgccatt ctgctctcct ttggtatcga caccgtgcag gcgatggcag gcaaagtgaa
    10621 ctggacggtg tacatcctgc aaaccggttt ctcctttgcg gtggcgattt tcatcatcac
    10681 tcagggcgtg cgcatgtttg tggcggaact ctctgaagca ttcaacggta tctcccagcg
    10741 cctgatcccg ggtgcggttc tggcgattga ctgtgcggct atttatagct tcgcgccgaa
    10801 cgccgtggtc tggggtttta tgtggggcac catcgggcaa ctgattgcgg ttggcatcct
    10861 ggtcgcctgt ggctcttcaa tcctgattat tccaggcttt atcccgatgt tcttctccaa
    10921 cgccactatc ggcgtgttcg ctaaccactt cggcggctgg cgcgcggcgc tgaagatttg
    10981 cctggtgatg gggatgatcg aaatctttgg ttgcgtctgg gcggtgaaac tcaccggtat
    11041 gagcgcctgg atgggcatgg cggactggtc gattctggca ccgccgatga tgcagggctt
    11101 cttctccatc ggtatcgcct ttatggccgt catcattgtc attgcactgg cttatatgtt
    11161 cttcgctggc cgcgcactgc gtgcagaaga agatgcagaa aaacaactgg cagaacaatc
    11221 tgcttaataa ggagttttga ttatgaccgt acgtattctg gctgtgtgtg gtaacggaca
    11281 aggcagttcc atgatcatga aaatgaaagt ggaccagttt ttaacccagt cgaacatcga
    11341 ccacacggta aacagctgcg cggttggcga atacaaaagc gagttgagcg gtgcggatat
    11401 catcatcgct tctacccata ttgcgggcga aatcaccgtg accggcaaca aatatgtggt
    11461 tggcgtgcgc aacatgctct ctcctgctga ttttggcccg aaactgctgg aagtgatcaa
    11521 agagcatttc ccgcaggacg tgaagtaagg acgcgccatg aaattacgtg attcgctggc
    11581 tgaaaataaa tctatccgcc tgcaagcgga agcagagaca tggcaggacg cagtgaaaat
    11641 cggcgttgat ctgctggtag cggcagatgt ggtagagccg cgttactacc aggcgattct
    11701 ggatgcggtt gaacagcacg gcccgtactt tgtgctcgca cccggcctgg caatgccgca
    11761 cggacgcccg gaagaggggg ttaagaaaac tggttttgcg ctggtgacgc tgaaaaagcc
    11821 gctggagttc aaccacgaag ataacgatcc ggtggatatc ctcatcacca tggcggcggt
    11881 tgatgccaac actcaccagg aagtgggcat catgcagatc gtcaacctgt ttgaagatga
    11941 agagaatttc gaccgtttac gtgcctgccg taccgagcag gaagtactgg atctcattga
    12001 ccgcaccaac gcggcagctt aagaaggaat tgaacatgtc attaccgatg ttacaagtcg
    12061 cgctggacaa ccagactatg gatagcgcct acgaaaccac tcgcctgatt gccgaagaag
    12121 tcgacattat cgaagtgggc accattcttt gcgtaggcga aggcgtgcgt gcggttcgtg
    12181 acctgaaagc gctgtacccg cacaaaatcg tactggctga cgccaaaatt gccgatgcag
    12241 gcaaaatcct ttctcgcatg tgcttcgaag ccaacgctga ctgggtgacc gtaatttgct
    12301 gtgcggatat caacaccgcc aaaggcgcgc tggacgtggc aaaagagttt aatggcgacg
    12361 tgcagatcga attgaccggc tactggacct gggaacaggc gcagcagtgg cgcgacgcag
    12421 gcattgggca ggtggtttat caccgcagcc gtgacgcgca ggccgcaggc gtggcatggg
    12481 gcgaagcgga cattaccgcg atcaaacgtc tttccgatat gggcttcaaa gtcaccgtca
    12541 ccggtggtct ggcgctggaa gatctgccgt tgtttaaagg cattccgatt catgtgttta
    12601 tcgctggtcg cagcattcgt gatgccgctt ctccggtgga ggccgcacgt cagttcaaac
    12661 gttccatcgc tgaactgtgg ggctaaggag cggatatgtt gtccaaacaa atcccgcttg
    12721 gcatctacga aaaagcgctc cccgctgggg agtgctggct ggaacgactg caactggcaa
    12781 aaacgttagg cttcgatttt gtcgagatgt cagtagatga aacggacgat cgcctttcgc
    12841 gcctcgactg gagccgcgag cagcgtctgg cactggtgaa tgcgattgtt gaaaccggcg
    12901 tgcgcgtacc gtccatgtgc ctttctgctc atcgtcgttt cccgctggga agcgaagatg
    12961 acgcggtgcg ggcccagggg ctggagatta tgcgtaaagc tatccagttc gcccaggacg
    13021 tcggtattcg cgtgatccaa ctggcgggct atgacgttta ctatcaggaa gccaataacg
    13081 aaacgcgtcg tcgtttccgt gacggcctga aagagagcgt tgagatggcg agccgcgcgc
    13141 aggtcacact ggcgatggag atcatggatt acccattgat gaactccatc agcaaggcgc
    13201 tgggctacgc acactatctc aacaatccgt ggttccagct ctacccggat atcggcaacc
    13261 tgtcggcgtg ggacaacgac gtgcagatgg agttgcaggc cggaatcggg catatcgtcg
    13321 cggtacatgt gaaagacacc aaacctggcg tcttcaaaaa cgtgccgttt ggcgaaggcg
    13381 tagtggattt cgaacgttgc ttcgaaacgc tcaaacagag tggctattgt gggccgtacc
    13441 tgattgagat gtggagcgaa acggcggaag acccggcggc agaagtagcg aaagcgcgtg
    13501 attgggtgaa agcgcgcatg gccaaagcag gcatggtgga ggcggcataa tgcaaaagct
    13561 gaaacagcag gtgtttgaag ccaacatgga tttaccgcat tacgggttgg tgacctttac
    13621 ctggggcaac gtcagcgcca ttgaccgtga acgcgggctg gtggtgatca agcccagcgg
    13681 tgtcgcctac gaaaccatga aagcggacga tatggtggtg gtcgatatga gcggtaaggt
    13741 ggtggaaggg gagtatcgtc cgtcttccga cactgcgacg catctcgaac tctaccgtcg
    13801 ttatccgtcg cttggtggta ttgtccatac ccactccact catgccaccg catgggcgca
    13861 ggcggggctg gcgatcccgg cgttaggcac tacgcacgcc gattacttct ttggcgacat
    13921 cccgtgcacg cgcggattaa gcgaagaaga ggtgcagggt gagtacgaac tgaataccgg
    13981 caaagtgatt atcgaaacgc tgggtgacgc cgagccgctg catacgccgg gaattgtggt
    14041 gtatcagcac ggaccgttcg cctgggggaa agatgcccac gacgcggtgc ataacgcggt
    14101 ggtgatggaa gaagtggcga aaatggcgtg gattgcgcgc agtattaatc cacaactcaa
    14161 tcacatcgac agcttcctga tgaataaaca cttcatgcgt aagcacggtc ccaacgccta
    14221 ttacgggcag aagtaaaaca cgcgctgcgg aaatttcctt cttcgggaga taactggtct
    14281 aattccgcag ccgtttttca aaaaaagccc cctgcgaagg gggcaaagca aactatggca
    14341 atgtttcgtt ggttatacct ggtgctagcg ataaatatcc gcgctggcgt gcatattgcc
    14401 gttactcccc ggttcccgca tcagaatcac atggtagtac gttgcgcctt gcgcatctgt
    14461 ctcttcattt aatgcctgac gtgcttcact ttcagtggcg aagttatggt tgatataaat
    14521 cacgcctaag ctttgcacat catccatatt tctggcctgg tggttattaa tttcaatggc
    14581 tgcccatgta tttgcactta gcaaaagcac agccagaagg gctaaaacac gactgaacat
    14641 agatacctcc ttgacggctg actttgtgtg ctctcctctt gtggtgatct tctgatttaa
    14701 ttttaatcaa tgataaagaa gttgatggtg accatttctg atgcagttgt tcaaaaaaac
    14761 accatgacga agtgtgatga actccaaatc agcggtttag aggttatttg cgaaagggga
    14821 gatttatttc ggctctgccc ttgagtttag cggggtatgt cagtactata acgcgtcatt
    14881 tttcagccga cctttaacac gttccttgcc tccccgggat tcggctgacc cagacaggag
    14941 gctgaataat ccgtaaggag caattcgatg cgtcattacg aaatcgtttt tatggtccat
    15001 cctgaccaga gcgaacaggt tccgggcatg atcgagcgct acactgctgc catcactggt
    15061 gcagaaggca agatccaccg tctggaagac tggggccgcc gtcagctggc ttacccgatc
    15121 aacaaactgc acaaagcaca ctacgttctg atgaacgttg aagctccgca ggaagtgatc
    15181 gatgagctgg aaactacctt ccgcttcaac gatgccgtta tccgcagcat ggttatgcgt
    15241 accaagcacg ctgttaccga agcatctccg atggttaaag cgaaagacga gcgccgtgag
    15301 cgtcgcgatg atttcgcaaa cgaaaccgct gatgatgctg aagctgggga ttctgaagag
    15361 taatttctga tgaccaaccg tctggtgttg tccggcaccg tgtgcaggac tccccttcga
    15421 aaggtcagtc catcaggaat tcctcactgc cagttcgtgc ttgagcatcg ttctgtgcag
    15481 gaggaagccg gctttcaccg gcaggcatgg tgccaaatgc ccgttattgt tagcggacac
    15541 gaaaaccagg ccattactca cagtataacg gtcggcagtc gcataaccgt tcaggggttc
    15601 atttcatgcc ataaggcaaa gaacggactg agcaaaatgg ttttgcatgc cgagcagatt
    15661 gaattgatag attctggaga ctagccatat ggcacgttat ttccgtcgtc gcaagttctg
    15721 ccgtttcacc gcggaaggcg ttcaagagat cgactataaa gatatcgcta cgctgaaaaa
    15781 ctacatcacc gaaagcggta agattgtccc aagccgtatc accggtaccc gtgcaaaata
    15841 ccagcgtcag ctggctcgcg ctatcaaacg cgctcgctac ctgtccctgc tgccgtacac
    15901 tgatcgccat cagtaatcgg tcacggtcca ttaatacgac tttgagagga taaggtaatg
    15961 caagttattc tgcttgataa agtagcaaac ctgggtagcc tgggtgatca ggtaaacgtt
    16021 aaagcgggct atgctcgtaa cttcctggta ccgcagggta aagctgttcc agctaccaag
    16081 aaaaacattg aattcttcga agcacgtcgc gctgaactgg aagctaaact ggctgaagtt
    16141 ctggcagcag ctaatgctcg cgctgagaaa atcaatgcac tggaaactgt taccatcgcg
    16201 tctaaagctg gcgacgaagg taaactgttc ggttccatcg gtactcgcga catcgctgac
    16261 gctgtaactg cagctggcgt tgaagtggct aagagcgaag ttcgtctgcc gaacggcgtt
    16321 ctgcgtacca ctggcgaaca cgaagtgagc ttccaggttc acagcgaagt attcgcgaaa
    16381 gtgatcgtaa acgtagtagc tgaataattc gttattcaac gagacgtaaa aacgccgacc
    16441 agtggtcggc gttttgcttt taagtgataa aaaccgcaga aacagacgtt ccctccataa
    16501 atctcttgct attcctttta taaaaagttc gcagtaccca tacctggccc ccagcctcgc
    16561 cgctggtagt tcgtgtcatg ttttttacgc gtaaaacacg acaaaagcat caatacagcg
    16621 atcgtctcac ttgtatgata ataaaaac
//
LOCUS       DAZFJL010000104        16497 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFJL010000104 DAZFJL010000000
VERSION     DAZFJL010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.104"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            247..630
                     /gene="iraD"
                     /locus_tag="ACHJ8C_003358"
     CDS             247..630
                     /gene="iraD"
                     /locus_tag="ACHJ8C_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO5553161.1"
                     /translation="MMRQSVQTVLPESTGNNTLSLRDSVCRDLFQLFSSPHSPLPILL
                     VSGMPEWQGHNQSDKLLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHELAISLEMM
                     LYHDDEPLTFDLVWQKGSWHRTMPQ"
     gene            complement(678..3059)
                     /gene="ptsP"
                     /locus_tag="ACHJ8C_003359"
     CDS             complement(678..3059)
                     /gene="ptsP"
                     /locus_tag="ACHJ8C_003359"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO5553162.1"
                     /translation="MVAIVIVSHSLRLAQGIEELALQMSGGDVPLAIAAGIDDPQNPI
                     GTDAIAIMSAIESVWSPDGVLVLMDMGSALLSTEMALELLSEEQRSAIYLLAAPVVEG
                     AMSAVTSAAAGLSVTEIIAEVDFALCAKQQQLMPSPPAGEVIPAISPVNHSGEWQTFC
                     WTICNPHGIHARPAASILKVSAQYSANIIVIKGDKRASTRSLNELAMLGVRCGDEIIF
                     QAEGTDASDALKAIETLAKNHFGEAHLLASSEIPLTETPVVSAPVDGAISGLSVQNGI
                     AIGPVKWFTCERPEITQRTVDSPQEELSRIESAIDIVVCELADKAAGPEGDIFAAHKM
                     MLEDPEINRQLQQRLAKGKQAEFAWLEVMQALAEQYCQAETLYLREREADIRDLTRQV
                     LNQLCGVSEQHFITTAPCILLANDLLPSQITSLNKAHILGICLHNGGTTSHTAILARA
                     MGIPAIVKAAITPQNVRDNDTVILDGETGRLWLQPDEVTRLDLLQRAEAWRQQRDRQL
                     ADAMLPAVTQGGRNISVLANIGDLQDIDAALSHGAEGVGLLRTEFLFHESATLPDEEE
                     QFRVYCSVARVFGDKPVTIRTLDIGGDKPLPSYPLPTEDNPFLGLRGIRLCLAHPQIF
                     IPQLRALIRAGKEYPTLQIMLPMVSTLEEVRAVKTLIHAQAKLLGLTAENLPALGIMI
                     EVPAAVMIADKLASEVDFFSIGTNDLTQYIMAADRGNSTVAKLVDYCNDAVINAIAMV
                     CQAGRNNEIPVSMCGEMAGDIQQTARLLTMGIDKLSASPSRLPALKAAIRTSH"
     gene            complement(3063..3695)
                     /gene="dhaL"
                     /locus_tag="ACHJ8C_003360"
     CDS             complement(3063..3695)
                     /gene="dhaL"
                     /locus_tag="ACHJ8C_003360"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO5553163.1"
                     /translation="MKIQNKHVIAWLESCAAHLTEQQDFLTALDRDIGDADHGLNMNR
                     GFSAVKATLPDIERQHIGNILKNTGMKLLSSVGGASGPLYGTLFIRASAAVGARTELS
                     LEEWLACLEEGIAGVIARGKAEQGDKTLCDVWVPVLHEAKKNLQAGMSPSLLLNTMVQ
                     DAATAVDNTINMQAKKGRASYLGARSVGHQDPGATSSWLMIKAMQEGFAG"
     gene            complement(3708..4772)
                     /gene="dhaK"
                     /locus_tag="ACHJ8C_003361"
     CDS             complement(3708..4772)
                     /gene="dhaK"
                     /locus_tag="ACHJ8C_003361"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO5553164.1"
                     /translation="MKKLINQIDSVVTEQMEGLIATWPHLQANYAPRYVWCKQTDNAV
                     ALISGGGSGHEPLHAGFVGMGMLTGACPGEIFTSPTPDQMIECAKAVDNGSGVLFFIK
                     NYTGDILNFETAVEMLHEEGIAVGTVIIDDDVAVKDSLYTAGRRGVAGTVFVEKIVGA
                     AALQGYNLGQCEQLGKDVNNATRSFGIALSACTVPAAGKPSFELADNEIEFGVGIHGE
                     PGIERRTLQDLNTLINSVIAQLLDNTPWRRTLRHWDRHAGGWIDASSMNESFDQNAEY
                     IVLINGLGSTPESELYGVARVFMCAAQRQGIKISRQLVGNYCTSLDMAGFSISLLKCT
                     PEFLQLWDAPVNTPALRWGC"
     gene            5098..6180
                     /locus_tag="ACHJ8C_003362"
     CDS             5098..6180
                     /locus_tag="ACHJ8C_003362"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HFO5553165.1"
                     /translation="MDKIIISPAKYIQGNGSLDNIATYAASLGTEPLIIADEFVTGLV
                     GDRVSQSFAKENITADFDIFCGECSQNEISRIREKFNQRKYNVVIGIGGGKTLDTAKA
                     VAYYQKIPVVVVPTIASTDAPTSSLAVIYTPEGQFSEYLFFPKNPDMVIMDTGVISAA
                     PVRLLVAGMGDALSTWFEARANQASGKATMAGGASTLAALAIARLCYTTLLEDGYKAK
                     VAVEQGVSTKAVENIIEANTYLSGIGFESSGLAAAHAIHNGMTQLEECHHCYHGEKVA
                     FGVLVQLVLENAPQEEIETVLNFCHSVGLPTNLHMLGVKEINKDKLRAVAEAATAEGE
                     TIHNMPFVVTAQDVLSAILTAHHLGL"
     gene            6246..7514
                     /locus_tag="ACHJ8C_003363"
     CDS             6246..7514
                     /locus_tag="ACHJ8C_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5553166.1"
                     /translation="MTSNWKRWITLALCGIAGSAIYKLPYLRETYYDAMQQATGATNA
                     ELGFLMTAYGLVNFLLYLPGGWAADRFSARKLMTFSLISTGISGFYYATFPSYTMICL
                     LHALWAVTTVFTFWAVCVRIIRTLGTSEEQGRLYGYWFLGKGLTSIVLGFLSVPVFAK
                     FGEGVDGLRATIIFYSVVTILAGVLAWFVCQDETHSEDKANFRLADMAFVLKMPTVWL
                     AGVVTFCMWSIYIGFGMVTPYLTQILHMGESEVAVASILRAYVLFAMGGLIGGQLADR
                     CASRTRFMIYAFIGMIVFTTVYFFLPGESRYVTIALANMVALGVFIYSANAVFFSIID
                     EVRIPAKVTGTAAGLISLLTYFPEIYCYTMVGNMVDRKPGIAGYQDVFLFMLVCAFIG
                     LIAALVLQRVNRKSKNQVNEITENNADCAS"
     gene            7532..8896
                     /locus_tag="ACHJ8C_003364"
     CDS             7532..8896
                     /locus_tag="ACHJ8C_003364"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5553167.1"
                     /translation="MVDMINESARQTPVIAQTDVLVIGGGPAGLSAAIAAGRLGARTM
                     IVERYGSLGGVLTQVGVESFAWYRHSGTEDCEGICREYEGRARALGFTRPEPQSISEV
                     IDTEGFKVVADQMITESGVEPLYHSWVVDVIKDGDTLCGVIVENKSGRGAILAKRIVD
                     CTGDADIAARAGAPWTKRSKDQLMGVTVMFSCAGVDVARFNRFVAEELKPTYADWGKN
                     WTIQTTGKEDPMFSPYMEDIFTRAQQDGVIPGDAQAIAGTWSTFSESGEAFQMNMVYA
                     FGFDCTDVFDLTKAEIAGRQQALWAIDALRHYVPGFENVRLRNFGATLGTRESRLIEG
                     EIRIADDYVLNQGRCSDSVGIFPEFIDGSGYLILPTTGRFFQIPYGCLVPQKVENLLV
                     AGRCISAGVVAHTSMRNMMCCAVTGEAAGTAAVVSLQQNCTVRQVAIPDLQNTLQQQG
                     VRLA"
     gene            9197..10738
                     /locus_tag="ACHJ8C_003365"
     CDS             9197..10738
                     /locus_tag="ACHJ8C_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFO5553168.1"
                     /translation="MHYLKKRFSLIELNVFIPAILFIAVIILCLTIYPQDTSQYINEI
                     HHFLTWEMGGIFLVMTFLVVLCCLWLAFSRYGDILLGQSGEKPDFSLLTWLGLIFTSG
                     TGGSLLYLASVEWIWIIQQPPFGAAAGSAQAARWASAYGMFHWGPSAWAWYLICAVPI
                     GWFMHVKKTNSLKVSDLCRGCLGARADGFCGHCVNFFYMFGLLGGAVTSLALGTPMIS
                     AVFCHVFHLDPAGQFINVMVIFIWTLVPLLILFFGLKKGVAWASNWNIRADILMLLAI
                     LICGPTAFILNQSIDGFGLMLQNFVAMSLSTDAIGRSGFPQMWTVFYFSWWVVYAIPF
                     GLFIARISKGRTIRQLIVCGTLAGSLGCMVFYMVLANFGLSLQTTHVIDFVPILNEQG
                     RGVVVSRLLEQLSASQVFLVAFGAIALISYITGHCTVGYALGFATQKRADSESEPAFW
                     NVAFWLIMTGIVAITLYLLDAQSLQPLQTVSILAGLPLCGVVFILLKSFLTQLAAEEK
                     TARDE"
     gene            complement(10794..11936)
                     /locus_tag="ACHJ8C_003366"
     CDS             complement(10794..11936)
                     /locus_tag="ACHJ8C_003366"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFO5553169.1"
                     /translation="MKIVISPDSFKECLPAWKVAEALATGWRKVLPGSQLVCLPVADG
                     GEGTLETLIHATDGTFYTKKVTGPLGESIHAQYGILGNQTTAVIELAQASGLELVSPV
                     QRSPLYTTSFGTGELILAALEHNIDTVILCLGGSATNDGGIGLMSALGASFTDAEGLS
                     VSVNGMGLAAIHHIDLQHLDPRLKNVKFIAACDVTNPLTGDNGATRVFGQQKGASADD
                     LEQLEQGMKNYARCIYRCCGKEVDTIPGSGAAGGVGAALMAFLDARLQPGISLVLEAI
                     QYTQHLKYAALAIVGEGKLDSQSLNGKAPVGAAKVAQMMGVPVIAIAGYIDDQLDLNE
                     LRQCGIEACFSVVNGPCNLPTALSQGENNLIRLGENLAGYFRAILS"
     gene            complement(12011..12898)
                     /locus_tag="ACHJ8C_003367"
     CDS             complement(12011..12898)
                     /locus_tag="ACHJ8C_003367"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO5553170.1"
                     /translation="MNIGFIGTGIMGKPMAYNLQQAGHTLYFSAHFESAPQELIGEHG
                     IVCSTPREVAQECEVIITMLPDTPHVEDVLFHPNYGVIHGLSHGKIVIDMSSISPVAT
                     KAFAQRIIAGGAEYIDAPVSGGEVGAKAGTLSIMVGGCEEVYLQIKPILELMGKNITL
                     VGNVGDGQTCKVANQIIVALNIEAVAEALLFASKSGADPARVREALMGGFASSRVLEV
                     HGERMIKGTFEPGFRISLHQKDLNLALENARLLNTPLPNTAMTQQLFSACAALGGKEW
                     DHSALIRALETQANFTIRK"
     gene            complement(12916..13698)
                     /gene="hyi"
                     /locus_tag="ACHJ8C_003368"
     CDS             complement(12916..13698)
                     /gene="hyi"
                     /locus_tag="ACHJ8C_003368"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO5553171.1"
                     /translation="MAKFAANLSMLFTEYPFIERFAQASHAGFHGVEYLFPYDFSADE
                     LVSQLHQHNLTQVLFNLPAGNWQEGERGIACHPGRAKEFQEGVSRAIEYALALNCSQV
                     NCLAGKHPGGYSHEQCHETLVENLRYAVDKLASHGIKLVLEAINTKDIPGFFVNNTRQ
                     ALNIIHDVNHPDFRYQYDIYHMQIMEGNIATTIKNNLNNIGHIQLADNPGRHEPGTGE
                     INYPWLLNYIDQIGYQGWIGCEYIPSTTTTESLRWLKNETQF"
     gene            complement(13711..15450)
                     /gene="gcl"
                     /locus_tag="ACHJ8C_003369"
     CDS             complement(13711..15450)
                     /gene="gcl"
                     /locus_tag="ACHJ8C_003369"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO5553172.1"
                     /translation="MARMRAIEAAVEILKKEGISVAFGVPGAAINPLYAAMKKSGGID
                     HILARHVEGASHMAEGYTRSQNGNIGVCIGTSGPAGTDMITGLYSASADSIPILCITG
                     QAPVGKLHKEDFQAVDIEAIATPVTKMARTILEAGQLPGIFQKAFWEMRSGRPGPVLL
                     DLPFDVQMTEIEFDIDLYQPLIPWQPQATRAQAVRALEMLNDAEKPVIIAGGGIINAE
                     ASELLREFVELTGVPVIQTLRGWGALSDDHPLMIGRMGCQAGHRYGNASYLASDFVFG
                     IGNRWANRHTGAIETYTEGRKFIHVDIEPAQIGRIFAPDLGIVSDAESALTLFIQVAR
                     DMKSRGELKDRSRWIAECAERKRTMLRRSDFDCNPIKPQRVYHEMNKVFGPETRYIST
                     IGLAQIAANQFLHVYRPRHWINACQAGPLGWTMPAALGAVKADPSVPVVAISGDYDFQ
                     FLIEELAVGAQFNLPYIHILLNNAYLGLIRQSQRAFDIDYCVQLSFENINAPEINGYG
                     VDHKAVVEGLGCKAIRVFASQDIAPALQEAQRLRDEFHVPVVVEIITERVTNIAMGPD
                     INKVTEFEEILDL"
     gene            15844..>16497
                     /locus_tag="ACHJ8C_003370"
     CDS             15844..>16497
                     /locus_tag="ACHJ8C_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFO5553173.1"
                     /translation="MNKESYHNDLKNKWKMFVKHGWVATNSTNHVMLRSWQKCLKHCD
                     PRHWNMPVKASGQTLQTIFSRNEEFIRISQRVVEDHFTLAGDDRLAFLIIDPHGWVLS
                     LNAAGDYSSQLRELGIESGMSWAEDGIGTNVYSLCRETNLYTQLEGAEHFSEQLHCYA
                     MSAAPVIDYYGNIHGYIVCIIETTAELVKLKTSYSCATEIANYIYIENEQKLINKVLC
                     "
BASE COUNT         4210 a         3736 c         4008 g         4543 t
ORIGIN      
        1 agaatatgtt aacgtatttc cgttatgttt aatgactttt gtcaaatgat acctttttaa
       61 attaaaagtc agtggatatt cttttctgta cagtgtttta tctttggcgg atcttataaa
      121 tcccttatga aagtgccagg gacgcaatga gcataaagga atgattatgg aatatgagca
      181 tcaacccagt ggtgtccggc agccagggca ggatacagat gccactaaac gtcaggagtg
      241 cgcaaaatga tgcgccaatc agttcagacg gtattacctg aaagtacagg aaacaatacg
      301 ttatcgctgc gtgattccgt atgccgggat ctctttcaac tttttagttc tccccattca
      361 ccgctaccaa tattacttgt ttcaggcatg cctgagtggc aagggcataa tcagagcgac
      421 aagcttctcc agagttggta ttgccgccag ttacgttccg cgcttttatt tcatgagcca
      481 cgtattgcgg cattgcaggt caatctgaaa gaagcctatt gccacgagtt ggccattagt
      541 ctggagatga tgctgtacca cgatgatgag ccgctaacgt ttgacctggt ctggcagaag
      601 gggagctggc atcgcacgat gccgcagtaa atgacgggaa acgcgatacg cgcaagacgt
      661 atcgcgttag gtcacaatta gtgggaggtt ctgattgctg ctttcaaggc cggaagccgg
      721 gacggactgg cactgagctt atcaatcccc atggtgagca gtcgtgctgt ttgctggata
      781 tctccagcca tttcgccgca catactgacc ggaatttcat tattacgtcc ggcctgacac
      841 accatagcaa tagcgttaat caccgcgtca ttacaataat cgaccagttt cgcgacggtg
      901 ctatttcctc tgtctgccgc cataatatat tgcgtcagat cgttggtgcc gatggaaaag
      961 aaatcgactt cactggctaa tttgtctgca atcatgactg cggcaggaac ctcaatcata
     1021 attcccagcg ccggaagatt ttctgcagta agtcccagca gttttgcctg agcatgtatc
     1081 aatgttttga cggccctgac ctcctcgagc gttgaaacca tcggcagcat aatttgcaac
     1141 gttgggtatt ctttcccggc cctgataagt gcgcgcaatt gaggaataaa tatttgtgga
     1201 tgcgccagac acaaccggat acctcgaagt cccaggaagg ggttgtcctc tgtgggtaaa
     1261 gggtatgaag gtagcggttt atcaccacca atgtccagag tccggatcgt gactggtttg
     1321 tcgccaaaaa ctcgggcgac agaacaatac acacggaact gctcttcttc atcgggtaat
     1381 gttgcagact catggaataa aaactcggta cgcagtaaac ccacgccctc agcgccgtga
     1441 gatagtgcgg catcaatgtc ttgcagatca ccaatattcg ccagcacgct gatgttacgc
     1501 ccaccttgcg tgacagcggg cagcatggca tcagctaact gacgatccct ttgttgccgc
     1561 catgcttcgg cgcgttgcag taaatccagg cgggtgacct catccggctg tagccacaaa
     1621 cggcctgttt caccatcaag aatgaccgtg tcgttatcgc ggacgttttg cggggtaatc
     1681 gccgctttca caatagcggg gattcccatt gctctggcaa ggatcgctgt atgtgaagtt
     1741 gttccgccgt tatgaagaca aatgccaaga atgtgtgctt tattcaggct ggttatctgt
     1801 gatggcaaca gatcgttggc taacaaaatg cagggcgcgg tagtgataaa gtgttgttca
     1861 cttaccccgc aaagctggtt cagtacctga cgtgtaaggt cacggatatc tgcctcgcgt
     1921 tcacgtaaat acagtgtttc cgcctgacaa tattgctctg ctaatgcctg cataacttct
     1981 aaccatgcga attccgcctg tttgcccttt gccagacgtt gctgtaattg ccggttaatc
     2041 tctggatctt caagcatcat tttatgggcg gcaaaaatat cgccttcagg gcctgccgct
     2101 ttatcggcaa gttcacaaac aacaatgtcg atagcagact cgattcgcga cagctcttct
     2161 tgcggagaat cgacggtgcg ctgcgtaatt tctggtcgtt cgcaggtaaa ccatttaaca
     2221 ggaccaatag cgataccatt ttgtactgac aagcctgata ttgcgccatc gacaggggcg
     2281 ctgaccaccg gcgtttccgt tagcggaatc tctgaagaag cgagcagatg agcttcacca
     2341 aaatgatttt tcgccaacgt ttcaattgcc ttgagagcgt cactggcatc ggtgccttcg
     2401 gcctggaaga tgatttcatc gccacagcgg acgcccagca ttgccagttc gttgagactg
     2461 cgggtgctgg cgcgtttatc gcctttgatg acgataatgt ttgccgaata ttgtgctgat
     2521 actttcagga tgctggctgc cggacgagca tgaatgccgt ggggattaca tatcgtccag
     2581 caaaaagttt gccattcacc ggaatgattg actggtgata ttgctggaat aacctcgcct
     2641 gcaggcgggg atggcatgag ctgctgctgt ttagcgcaca gcgcaaaatc cacttcagcg
     2701 ataatctccg tcaccgaaag ccccgctgcg gcagacgtta ccgctgacat ggcaccttcc
     2761 actacagggg cggcgagcag ataaattgca cttctttgct cttcagaaag cagctccagc
     2821 gccatctcgg tactaagcag cgcactgccc atatccatga gtaccagaac gccatccggt
     2881 gaccagaccg attcaatcgc agacataatg gcaatagcat cagtgccaat cggattttgc
     2941 ggatcatcaa tacctgcagc aatggccaga ggaacgtccc ctccagacat ctgtagtgct
     3001 aattcctcaa tcccctgggc gagacgtaga ctatgagaaa caataacaat ggcaaccata
     3061 atttatcccg cgaatccttc ctgcatagcc ttaatcatca gccaggagga ggtcgcgcct
     3121 ggatcctgat ggcccacact gcgagcgccg agataactgg cacgtccttt tttcgcctgc
     3181 atattgatgg tgttatcgac ggctgttgcc gcatcttgca ccatcgtatt cagcagaagg
     3241 gaaggcgaca ttccggcttg caggttcttt ttggcttcgt ggaggactgg aacccagaca
     3301 tcacacagtg ttttatcccc ttgttctgct tttcctcgtg cgataacgcc tgcaatccct
     3361 tcttccagac acgccaacca ctcttccaga cttaattctg ttctggctcc cactgccgca
     3421 gaagcgcgaa taaataatgt gccgtaaaga ggaccactgg caccgccaac gctggaaagt
     3481 agtttcatgc cagtattttt aaggatattg ccaatatgct gacgttcaat gtcgggcaac
     3541 gtcgctttca cggcactgaa accacgattc atattgaggc cgtggtcggc atcaccaata
     3601 tctctgtcaa gtgcggttag aaaatcttgt tgttcagtaa gatgtgcagc acaactctcc
     3661 agccaggcaa taacatgttt gttttgaatt ttcatataat gtccttatta acatccccaa
     3721 cgaagcgctg gcgtattgac cggggcatcc caaagttgca aaaactctgg ggtacatttc
     3781 agcaggctaa tggaaaatcc ggccatatct aatgatgtac aataattacc gaccaactgg
     3841 cggctaattt taattccctg gcgctgtgcg gcgcacataa ataccctggc tacaccatat
     3901 aactctgatt caggcgtgct gccaagtccg ttaattaaca caatatattc ggcattttga
     3961 tcgaatgact cattcattga tgatgcgtca atccatcccc ccgcatggcg atcccaatgg
     4021 cgtaatgtgc gtcgccaggg ggtattatcc agtaattgcg ctatcacgct gttgattaaa
     4081 gtgttcagat cttgcaaagt gcggcgttca atgccgggtt caccgtggat cccgacacca
     4141 aattcgattt cattatcagc cagttcaaat gagggttttc ccgcggcggg aaccgtacag
     4201 gctgacagtg cgatgccaaa agaacgggtg gcattgttaa catctttgcc aagttgctcg
     4261 cactgcccca ggttatatcc ctgaagtgca gccgcaccga caatcttttc aacaaaaact
     4321 gtacccgcaa caccgcgacg tccggctgta tacaggctat cttttaccgc tacatcatca
     4381 tcaataatga cggtcccaac ggcgatgcct tcttcgtgta acatctcgac tgccgtttcg
     4441 aaattcagaa tatcgccagt gtaatttttg atgaaaaaca gaacgccaga accattatca
     4501 accgctttgg cacattcgat catttgatct ggtgtgggcg atgtgaagat ttcaccagga
     4561 catgcaccgg ttaacattcc cataccgaca aaacctgcat gcagtggttc atggcctgag
     4621 ccgccgcctg aaatcagcgc cactgcatta tcggtttgtt tgcaccacac atatcggggc
     4681 gcgtaatttg cctgcaggtg tggccaggtg gcgatgagtc cttccatttg ttctgtgaca
     4741 acagaatcaa tctggttaat taatttcttc atagtcattc ctgttctgat aaattacgta
     4801 aatcgcgcta aatatgcttg cgagattaat tctccagccg caataaaaca aatcgcacga
     4861 taataagatt cataatgaaa cagttaattc atggtgttgt ttcaaaatga aaagggacca
     4921 gcgtggatat tgatatattg tgattaaggc atgaaaagta ttttttatta tcatctgcag
     4981 atgatgtttt ttacagcgcg atcataaaac ataatttttc gcgaatgaaa tgtttgccgc
     5041 cactggatgc aacgagtagg ttaacagtga agcaaaacaa gttacgcgag gaatgatatg
     5101 gataagatta ttataagtcc ggctaaatat atccagggaa atggttctct tgataatatt
     5161 gctacatatg cggcgagttt gggaacagaa ccattaatta ttgcagatga atttgtgacc
     5221 ggtctggtgg gtgacagagt aagccagagc tttgcaaaag aaaatattac tgcagatttt
     5281 gatatctttt gtggtgaatg ctcgcaaaat gaaatttctc gaattcgtga gaaatttaat
     5341 cagcgtaaat ataatgtcgt tattggtatt ggcggcggta aaacgttgga tacggcaaaa
     5401 gccgtagcgt attaccaaaa aattccggtg gtggtggtgc cgacaattgc ttccacagat
     5461 gcgccaacca gttctctggc agtaatttac accccggaag gtcagtttag cgaatatctg
     5521 tttttcccga aaaacccgga tatggtgatt atggatactg gcgtcatttc agccgcgcca
     5581 gtgcgtctgc tggtggccgg tatgggagat gcattgtcaa cctggtttga ggcgagagcc
     5641 aatcaggcgt caggaaaagc cacaatggca ggtggggcgt cgacactggc ggcactggct
     5701 attgcccgac tctgttatac cacgctactg gaagatggct ataaggcaaa agtcgcggtt
     5761 gaacaaggtg ttagcaccaa agcggtagag aatattatcg aagctaatac ctatcttagc
     5821 ggcattggat ttgaaagtag cggcctggcg gcagctcatg ccattcataa cggcatgacg
     5881 caactggagg aatgccatca ttgctaccac ggtgaaaaag tggcatttgg cgtcctggta
     5941 cagctcgtac tggaaaacgc accacaggag gaaattgaaa ccgtactcaa tttctgccat
     6001 agcgttggtt tgccgacaaa tcttcatatg ttaggcgtga aggaaattaa taaagataaa
     6061 ttacgggcag tggctgaagc cgcgactgct gaaggggaaa ctatccataa tatgccattc
     6121 gtggtcactg cgcaggacgt tctttccgca attttaaccg cgcatcattt aggactgtaa
     6181 aaataatacg gcctgtgcaa ggcaggccac taatgacatt tttaattctt cagtcgggaa
     6241 taaatatgac cagtaactgg aaacgttgga taacgctggc tttatgcggc attgccggaa
     6301 gtgcaatata taaattacct tatcttcggg aaacctatta cgacgcaatg cagcaggcta
     6361 ccggagcaac caacgctgaa ctcggttttt taatgacagc ctatggattg gtgaactttt
     6421 tattatatct acctggcggc tgggctgccg atagattttc tgcacgtaaa ttaatgacct
     6481 tttcattaat ttccaccggg ataagtggtt tttattatgc gaccttcccc tcgtatacta
     6541 tgatctgttt gttacatgca ctttgggctg ttacaaccgt atttacgttt tgggccgtgt
     6601 gtgtgcgaat tattcgtacc ctgggaacca gcgaagaaca aggtcgctta tacggttact
     6661 ggtttctggg taagggatta acctccattg tactgggatt tctttccgtg ccggtatttg
     6721 ccaaatttgg cgaaggtgtt gatggtttac gggcgacaat tattttttat tccgtagtga
     6781 ctattctggc aggtgtttta gcctggtttg tttgtcagga tgaaacgcac agcgaagata
     6841 aagcgaattt ccgtctggca gatatggcat tcgtgctgaa aatgccgacg gtttggctgg
     6901 caggtgttgt gaccttttgt atgtggtcaa tttatatcgg ctttggcatg gtcacgccgt
     6961 atctcacgca gatcctgcat atgggcgaat cggaagtggc agttgccagt attcttcgcg
     7021 cctatgtgtt atttgcgatg gggggattaa ttggcggaca actggccgac cgctgtgcgt
     7081 cacgcacgcg ttttatgatt tacgccttta tcggcatgat tgtctttact accgtttatt
     7141 tcttcctgcc cggtgagtcg cgctacgtca ctattgcgct ggcgaatatg gttgcgttgg
     7201 gcgtgtttat ttactctgcg aatgccgtgt tcttctcaat tattgatgag gttcggatcc
     7261 cggcaaaagt cacagggact gctgcgggtc tgatctcttt actgacctat ttcccggaaa
     7321 tctactgcta cacgatggtg ggcaatatgg tcgaccgcaa accgggtatt gccggttatc
     7381 aggatgtttt cctgttcatg ttggtttgcg cattcatcgg ccttatcgcc gcactggtcc
     7441 tccagcgtgt gaatcgtaaa agtaaaaatc aagtcaatga aatcactgaa aacaatgcgg
     7501 attgcgcatc ctgaatctga cgggggaacc aatggtggac atgattaatg aaagtgcacg
     7561 gcaaacgcca gtcattgcac aaacggacgt tctggttatc gggggcggtc cggcaggatt
     7621 atccgctgcc attgcggcag ggcggttagg tgccagaacc atgattgttg agcgctacgg
     7681 gtcgctaggc ggcgtattga cgcaggtcgg ggtagaaagt tttgcctggt atcgtcattc
     7741 ggggacggaa gattgtgaag ggatctgtcg tgagtatgaa ggccgcgcac gagcgctggg
     7801 tttcacacga ccagaacctc agtcaattag cgaagttata gatactgaag gatttaaagt
     7861 tgtcgccgat cagatgatta cggaatctgg cgttgagccg ttatatcact cctgggttgt
     7921 ggacgtgatc aaggacgggg atacgttatg cggtgttatc gtcgagaata aatcaggtcg
     7981 aggggcaatt ctggcgaaaa gaatcgtcga ttgcacgggg gatgctgata ttgccgctcg
     8041 tgcaggcgcg ccctggacga aacggagcaa ggaccaactg atgggcgtca ccgtgatgtt
     8101 cagttgcgca ggtgttgatg tggcacgctt taaccgtttt gttgcggaag aacttaagcc
     8161 gacctacgcg gattggggca aaaactggac gattcaaacc acgggtaaag aagacccgat
     8221 gtttagcccg tatatggagg atatttttac ccgcgcgcaa caggatggtg tgattccagg
     8281 tgacgcccag gcgattgccg gaacctggtc gaccttttct gaaagcggtg aggctttcca
     8341 gatgaatatg gtgtacgcct ttggttttga ctgtaccgat gtcttcgatt taaccaaagc
     8401 tgagattgcc ggaaggcagc aagcattatg ggcaattgac gcactacgcc actatgttcc
     8461 gggctttgaa aatgtacggt tacgcaattt tggtgccacg ctggggacgc gtgaatcacg
     8521 gcttattgag ggggaaatac gtattgctga tgattacgtc cttaatcagg ggcgttgttc
     8581 ggacagtgta gggattttcc cggaatttat tgatggttcc ggttatctca ttttgccaac
     8641 gaccgggcgt ttctttcaga tcccttatgg ttgtctggtg ccgcaaaaag tggagaacct
     8701 tttggtcgcc ggtcgctgta tttccgcagg cgtagttgca catacttcta tgcgtaacat
     8761 gatgtgttgt gccgttaccg gtgaggccgc aggtactgcc gccgtggttt cgctacagca
     8821 aaattgcacc gtgcgtcagg ttgctatccc tgatttgcaa aacacgctgc aacagcaggg
     8881 cgtacgtctg gcataacaac aaggcgcaga gagctgaaag cctgtctgcg ccttgcagga
     8941 gaaagaccat gagggatgat gacctggagc acttaagtgc caattactgg ataaccacag
     9001 ttctctgcgg cctctttttg gggatattcc tgttcatgct gcaacagcat cagcgtggtt
     9061 tagctctgta ttgtattgcg gctctttcac ttatttgcgc ctttttttct ttacgccttt
     9121 cccgacaatt gcaacacatg aaaaaggaac ggcgaaatat tcgacctcat tgaatgaaaa
     9181 cattcaggag actgaaatgc actatttaaa aaaacgcttt tctttgattg aactgaatgt
     9241 gtttattcca gcaatattat ttattgcggt aatcattctg tgcttgacga tatatccgca
     9301 agataccagt cagtatataa atgaaataca tcatttttta acctgggaaa tgggcgggat
     9361 ttttctggtc atgacttttc tggttgtact ttgttgtctg tggctggcct tctcacgtta
     9421 tggcgatatc ttgttaggtc agtcgggaga aaaacctgac ttcagcttgt taacctggct
     9481 tggacttatt tttacttctg gaacgggggg cagcttgtta tatctggcct ctgtagagtg
     9541 gatttggatc attcagcaac cgccttttgg cgcggcagca ggaagcgctc aggctgctcg
     9601 ttgggcctct gcttatggca tgttccattg ggggccgtcc gcatgggcat ggtatctgat
     9661 ttgtgccgtt cccattggtt ggtttatgca tgttaagaaa acgaactcat taaaggtcag
     9721 tgatttatgc cgtgggtgtc tgggggcacg tgcggatggt ttttgcgggc attgtgtgaa
     9781 ttttttctac atgtttggtt tgctcggcgg cgcggtaacg tctctggcgc tgggaacgcc
     9841 gatgatttct gccgtatttt gccatgtgtt ccatctggat cctgccgggc agtttatcaa
     9901 tgtcatggtt atttttatct ggacgctagt gccattatta attctctttt ttggacttaa
     9961 aaaaggtgtg gcatgggcca gtaactggaa tattcgtgct gacattttga tgctactggc
    10021 aatactgatt tgtggaccga cagcttttat acttaaccaa tcaattgatg gtttcggcct
    10081 gatgctgcaa aattttgtag cgatgagttt aagtaccgat gctattggtc gtagcggatt
    10141 tccacagatg tggaccgtat tttatttttc atggtgggtc gtgtatgcca tcccattcgg
    10201 tttatttatc gcccgtattt caaaaggaag gacgatccgg caattgattg tatgtggaac
    10261 tctggcaggt tcattgggat gcatggtttt ttacatggta ctggctaatt tcggcttatc
    10321 gttgcagaca actcatgtca tcgattttgt tcccatactt aacgaacaag ggcgaggcgt
    10381 tgtcgtttct cgtttactgg agcagctatc cgcaagtcag gtgtttttgg ttgcttttgg
    10441 ggctatagca ttaatttcat atattaccgg acactgtact gtgggttatg ccctcggttt
    10501 tgcgacgcaa aaacgggcag atagtgagag tgaaccggca ttctggaacg tggcattttg
    10561 gttgattatg actggaatcg tcgccatcac actctatctt cttgatgcgc agagtttgca
    10621 gccgctacaa acggtctcta tcctggccgg actaccgctt tgcggcgtag tgtttatttt
    10681 attgaagagt tttttgacac agcttgcggc tgaagagaaa accgcgagag atgaataatg
    10741 atgttcagga atgcccggta atttgcatta tcgggcattt taccgaacgg gaattaactc
    10801 aggatcgcac gaaaatatcc cgccagattt tctccaagac gaattaaatt attttctccc
    10861 tgactcagcg cggtgggtaa attgcaaggg ccgttgacga cagaaaaaca ggcttctatt
    10921 ccacactggc gtaactcatt cagatcaagt tgatcatcga tatacccggc aattgcgata
    10981 accggaacgc ccatcatctg ggcaactttg gctgcgccta cgggcgcttt gccattcagg
    11041 ctctgactat ctaatttccc ttcaccgaca atggccaatg ctgcgtattt taaatgttgg
    11101 gtatattgaa tcgcttccag cacgagcgaa atacccggtt gtaagcgagc atcgagaaaa
    11161 gccatcaagg ccgcgccaac gccgccagcc gccccagaac cgggtatcgt atcgacttct
    11221 ttaccacaac aacggtagat gcaacgggca tagtttttca ttccctgttc cagttgctca
    11281 aggtcgtcag cactggcccc tttttgttga ccgaaaaccc gggtcgcgcc gttatcgccg
    11341 gttagtgggt tggtgacatc gcaggctgca ataaatttca catttttcaa tcgtggatcg
    11401 aggtgctgta agtcaatgtg gtgaattgcc gccagcccca tcccattaac agagactgat
    11461 agcccttcgg cgtctgtaaa cgatgcgcca agtgccgaca tcaacccaat accgccatca
    11521 tttgtagcac tgccacccag gcatagaata acggtatcaa tattgtgttc cagggcggcg
    11581 agaataagtt cgccagtgcc aaacgacgtc gtataaaggg gagaacgctg gacaggagaa
    11641 accagttcca gccctgaagc ttgtgccagt tctatcactg cggtggtttg gttgcctaaa
    11701 ataccgtatt gcgcatgtat tgattcgcca agcggtccgg tgactttttt agtgtaaaac
    11761 gtaccgtcag tcgcatgaat gagtgtttcg agcgtgcctt cgccgccgtc agccacgggc
    11821 aaacacacca gttggctgcc aggcaggacc ttgcgccagc cggttgccag tgcttcagcg
    11881 actttccatg caggaaggca ttctttaaag gagtcggggg agataactat cttcatagca
    11941 ggtctctcaa aataggggaa tggggcaggg caatgagtga acgaagggaa ccggtggttc
    12001 ccttaattca ttatttacgg atagtgaaat tggcttgtgt ttcaagcgcc cgaattaacg
    12061 cagaatgatc ccactcttta ccaccgagcg cggcgcaggc gctaaataat tgttgcgtca
    12121 tcgccgtgtt aggaagcggg gtattcaaca ggcgagcatt ctccagtgcc agattcagat
    12181 ctttttgatg cagactaata cgaaaaccgg gttcgaatgt tcctttaatc atccgttcgc
    12241 catgaacttc cagcacgcga gaggaggcaa accctcccat cagcgcttca cgtacgcggg
    12301 cgggatcggc acctgattta ctggcaaaga gcagggcttc agccaccgct tcaatattca
    12361 gcgcaacaat aatttggtta gcgactttgc acgtttgacc atcaccgacg ttaccgacca
    12421 gagtgatatt tttacccatc aattccagaa tcggtttgat ctgcaaataa acctcttcac
    12481 atccgccaac cataatggaa agcgtcccgg ctttcgcgcc aacttcaccg ccagagacgg
    12541 gcgcatcgat atattccgcg cctcccgcga taatacgttg tgcaaatgct ttggtggcaa
    12601 ccggggaaat tgaactcata tcaatcacaa ttttcccgtg gcttaaaccg tgtattacgc
    12661 catagttggg atgaaaaaga acatcctcaa catgtggagt atcagggagc atggtgatga
    12721 tgacctcaca ctcctgcgcg acttcacgcg gtgtagagca aacaatgcca tgttcaccaa
    12781 taagctcttg tggtgcagat tcaaaatgcg ctgaaaaata aagagtatgt ccggcttgtt
    12841 gtaaattata agccatcggc ttacccatta tgccggtgcc gataaatcca atattcatga
    12901 taaatacctc agggattaaa attgagtctc gttttttagc cagcgtagac tttcagtcgt
    12961 tgtcgtagat ggaatatatt cgcaaccaat ccatccctga taaccaatct ggtctatata
    13021 atttaacaac cacgggtaat taatttctcc tgtccctggt tcatgacgcc cagggttatc
    13081 ggctaattga atatgtccaa tgttattcag gttgtttttg attgttgtag caatattccc
    13141 ttccatgatt tgcatatgat aaatatcgta ttgatagcga aaatcaggat gatttacatc
    13201 atgaataata ttgagagctt gtctggtgtt gttaacaaaa aaaccgggaa tatcttttgt
    13261 attaatggct tccagaacta actttatccc atgagaagcc aatttatcta ccgcatagcg
    13321 caaattttcg accagtgttt catgacattg ttcatgcgaa taaccacctg gatgttttcc
    13381 tgcaaggcaa ttaacttgtg aacaattaag ggccaatgca tactctattg ccctggaaac
    13441 accctcctga aattcttttg cccgaccagg atgacatgca ataccccgtt caccctcttg
    13501 ccagttaccc gcaggaagat taaacagaac ctgcgtcaga ttatgttgat gcagttgcga
    13561 aaccaactca tctgcggaaa agtcataggg gaaaagatac tcaacgccgt ggaatcctgc
    13621 gtgacttgcc tgtgcaaaac gttcaataaa tggatactcg gtaaagagca tagataaatt
    13681 tgctgcaaac tttgccataa tcctgtttcc ttataaatcg agaatttctt caaattctgt
    13741 gactttatta atgtcaggtc ccatagcgat attagtcacg cgttcagtaa taatttccac
    13801 aacaacaggt acgtgaaatt cgtcacgcaa acgctgggct tcttgcagtg caggcgcaat
    13861 atcctgtgat gcgaatacgc gaatcgcttt acatcctaat ccttcaacca cagctttatg
    13921 atcaacgcca taaccattaa tttctggagc attaatattt tcaaatgaca gttgaacgca
    13981 ataatcaata tcaaatgcac gttgtgattg acgaatcaaa ccaagataag cgttgttaag
    14041 taaaatatgg atatacggaa gattaaattg tgcgccgacg gctaattctt caatcaagaa
    14101 ttgaaaatcg taatcaccgg atattgccac aacgggaaca gatggatcag cttttaccgc
    14161 gcccagtgct gcgggcattg tccagcctag cggcccagcc tgacaggcat taatccagtg
    14221 gcgtgggcgg taaacatgta aaaattggtt tgcggcgatt tgcgccagac caattgtcga
    14281 aatatagcgt gtctccggtc cgaaaacctt attcatttcg tgatacacgc gctgcggttt
    14341 aattggattg cagtcaaagt ctgaacgacg aagcattgtg cgtttacgct cggcacattc
    14401 ggcaatccag cgactgcggt ctttcagttc tccacgtgac ttcatatcac gggcgacctg
    14461 aataaatagc gttaaggcgc tttcagcatc agaaacaatg cccagatccg gcgcaaatat
    14521 ccggccaatt tgtgcaggtt cgatatcaac atgaatgaat ttgcggcctt cggtgtaggt
    14581 ctcaatggct ccggtatggc ggtttgccca acggttaccg ataccaaaaa caaaatctga
    14641 ggccagataa ctggcgttac catagcgatg acccgcctga catcccatgc gaccaatcat
    14701 tagggggtga tcgtctgata aagcgcccca accgcgtaag gtttgaatga cgggaacgcc
    14761 agttaactcg acaaactcac gcaacaattc actcgcttcg gcgttaataa tgccaccgcc
    14821 agcgatgatg acgggctttt cggcgtcatt tagcatttct aatgcgcgta cggcctgggc
    14881 ccgtgttgcc tgaggttgcc aggggattag tggctgatag agatcgatat caaactcaat
    14941 ttcggtcatc tgaacatcaa aagggagatc taaaagaacc gggccagggc ggcctgaacg
    15001 catttcccag aaggctttct ggaatatccc cggcaactga cccgcttcga gaattgtgcg
    15061 agccattttg gttactggcg tggcgatagc ctcaatatcg acggcctgga aatcttcttt
    15121 atgaagtttt cctacgggag cttgaccagt aatgcagaga ataggaatgg aatccgcaga
    15181 ggcagaatac agcccggtaa tcatatcagt gcccgcaggg cctgatgtgc caatacacac
    15241 gccaatattg ccattctgac tgcgggtgta accttcagcc atatgcgagg cgccctcaac
    15301 atgacgggcc agaatatgat caataccgcc tgattttttc atcgcggcat agagtgggtt
    15361 tattgctgcg ccgggtacac caaaagcaac gctaatgcct tcttttttca gtatttctac
    15421 tgcagcttcg attgcacgca tcctcgccat acattatttc tcctgatatt tacgctgagt
    15481 tgaatagtca gtcaattttg gaatttaacg atgaaatatc tttgaggaaa taaaagggca
    15541 gaacaaattt cgctgcactg tttcacagtg aaacaactcg tatgcctgtg ttgtttcagt
    15601 ttgaataatt ataaatatca aacgcaaaga tttcagatgt tgttggcagt tttaaaattt
    15661 atctaatgta tcattcgttt tttattgctt taacggttat tatttctaat gtatgtgtga
    15721 ttacattgtt gataaggtct gtttttgtaa tgatccaggc tagatattat tgcgtttgat
    15781 caagcttact ggcatagcat tctgatacaa gttctgaaaa tgacttgaat ggatattatt
    15841 ataatgaata aagagtcgta tcataatgat ctaaaaaata aatggaaaat gtttgttaag
    15901 catggttggg tcgcgacaaa ttcgactaat catgtgatgc tgaggtcatg gcaaaaatgt
    15961 ctcaagcatt gtgaccctcg ccactggaac atgcctgtca aagcttccgg gcaaacattg
    16021 cagacgattt tttctcgtaa cgaagagttt attagaattt cacaaagagt tgttgaagac
    16081 catttcaccc tggctggtga cgaccggttg gcatttttga tcattgatcc acacggctgg
    16141 gttctatcgt tgaatgcagc aggcgattat tccagtcaat tgcgagagtt aggaattgaa
    16201 tctggtatgt catgggccga agatggcatt ggaaccaatg tatatagtct ttgtcgggaa
    16261 acaaatttat atacacaact ggaaggagca gaacatttta gtgaacagct acattgttat
    16321 gcgatgagcg ctgctccggt cattgattat tatggtaata tacatggata cattgtatgt
    16381 ataattgaaa ccaccgccga gcttgttaaa ttaaaaacat catattcatg tgcaacagaa
    16441 atcgctaatt atatctatat tgagaatgaa caaaagttaa taaacaaagt actttgc
//
LOCUS       DAZFJL010000105        16024 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZFJL010000105 DAZFJL010000000
VERSION     DAZFJL010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.105"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..521
                     /locus_tag="ACHJ8C_003371"
     CDS             <1..521
                     /locus_tag="ACHJ8C_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO5553174.1"
                     /translation="AMAISSVLLLGAARFLPALQRESLTNTRKLALEDEIWLRVFTVA
                     KHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREPVKESDQIGFRLKEHV
                     LETLRGATSCEGKDWDKVTNPDAIIIDTFQVVRQDVSGFSPVLTVNMHAASKADPQTV
                     VDASYSVTGSNL"
     gene            518..925
                     /locus_tag="ACHJ8C_003372"
     CDS             518..925
                     /locus_tag="ACHJ8C_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO5553175.1"
                     /translation="MNREKGVSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTLPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            910..1233
                     /locus_tag="ACHJ8C_003373"
     CDS             910..1233
                     /locus_tag="ACHJ8C_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO5553176.1"
                     /translation="MSASLRNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            1246..4614
                     /gene="recC"
                     /locus_tag="ACHJ8C_003374"
     CDS             1246..4614
                     /gene="recC"
                     /locus_tag="ACHJ8C_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO5553177.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWERGHLVEGLGE
                     AQAWQAPLWKALVEYTDELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSIIFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLSS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCHHTRMPFDPQNYQPGN
                     LQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNVLVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDVEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGADI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            4790..7678
                     /gene="ptrA"
                     /locus_tag="ACHJ8C_003375"
     CDS             4790..7678
                     /gene="ptrA"
                     /locus_tag="ACHJ8C_003375"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO5553178.1"
                     /translation="MPRSTWFKALLLFVALWAPFSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQKKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATMLARHVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            7671..11213
                     /gene="recB"
                     /locus_tag="ACHJ8C_003376"
     CDS             7671..11213
                     /gene="recB"
                     /locus_tag="ACHJ8C_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO5553179.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYKRLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLH
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFEPQWEPVLTEWITTVLLAPLNETGVSLNQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYATRPNAGLIDLMDEMFAGMTLEEA"
     gene            11213..13039
                     /gene="recD"
                     /locus_tag="ACHJ8C_003377"
     CDS             11213..13039
                     /gene="recD"
                     /locus_tag="ACHJ8C_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO5553180.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTPERAGQLSRLTG
                     THVPAGTGTDAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPNLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPNQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(13101..14432)
                     /gene="argA"
                     /locus_tag="ACHJ8C_003378"
     CDS             complement(13101..14432)
                     /gene="argA"
                     /locus_tag="ACHJ8C_003378"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO5553181.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            14664..15917
                     /gene="amiC"
                     /locus_tag="ACHJ8C_003379"
     CDS             14664..15917
                     /gene="amiC"
                     /locus_tag="ACHJ8C_003379"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO5553182.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
BASE COUNT         3762 a         3985 c         4498 g         3779 t
ORIGIN      
        1 ttgctatggc gatcagtagc gtattgttgc tgggggctgc acgctttctg cctgcgttac
       61 agcgtgaaag tttaacgaac acccgtaagc tggcgctgga agatgaaatc tggctgcggg
      121 tatttaccgt cgcgaagcat ctccagaggg cgggttattg ccatggcagc tgtacgggcg
      181 aagggctgga aattgtcgga cagggtgact gtgtcattgt gcagtgggat gcgaacagta
      241 atggtatctg ggatcgcgaa ccggtaaaag agtccgacca gattggattt cgtctgaagg
      301 agcatgtgct ggaaacgcta cgcggtgcga catcctgtga aggtaaggac tgggataaag
      361 tcactaatcc ggatgccatc attatcgaca cttttcaggt cgtacgtcag gatgtcagcg
      421 gcttctcgcc ggtgttgacg gttaatatgc acgctgccag caaggctgat ccgcaaaccg
      481 tggtggatgc cagctatagc gtgacaggat ccaacctgtg aaccgcgaaa agggtgtttc
      541 gtcactggct ctggtcctga tgctgctgat tttgggtagc ttgctattgc aaggaatgag
      601 tcagcaggat cgcagttttg cttctcgcgt gagcatggaa agtcagtcat tgcgccgcca
      661 ggccatcgtt cagtcggcgc tggagtgggg aaaaatgcac tcctggcaga cgctgcccgc
      721 agttcagtgc ttactgtatg ctgccaccgg tgcccgggtt tgtctgcgtt tactggcaga
      781 taatgaagcc ttattgattg ctggttatga aggcgtttcg ttgtggcgaa caggcgaagt
      841 catcgatgga aacattgttt tttcgccacg cggctggagc gatttttgtc cgctgaaaga
      901 gagggcgtta tgtcagcttc cctgaggaat caacaaggct ttagtctgcc ggaggtaatg
      961 ttggcgatgg tgttgatggt gatgattgtc actgcgttat cgggtttcca gcgaacatta
     1021 atgaacagtc ttgccagcag aaaccagtac caacagctct ggcggcatgg ctggcagcaa
     1081 acgcaactgc gcgcgatttc gccacctgct aactggcagg tcaaccgaat gcagacatcg
     1141 caggcgggat gtgtcagcat cagcgttacg ctagtttcac ccgggggcag agaaggcgag
     1201 atgacccgcc tgcattgccc gaatcgtcag tagtcaggag ccgctatgtt aagggtctac
     1261 cattccaatc gtctggacgt gctggaagcg ttgatggagt ttattgtcga acgcgaacga
     1321 ctggacgatc ctttcgaacc agagatgatt ctggtgcaaa gtactggtat ggcacagtgg
     1381 ctgcaaatga ccctgtcgca aaagtttggt attgcggcaa acattgattt tccgctgcca
     1441 gcgagcttta tctgggatat gttcgtccgg gtattaccgg agatccccaa agagagcgcc
     1501 tttaacaaac agagcatgag ctggaaactg atgactctgc tgccgcaact gttggagcgc
     1561 gaagacttta ccctgttgcg gcattatctg actgacgata gtgacaagcg aaaactgttc
     1621 cagctttctt caaaagcggc ggacctgttt gaccagtatc tggtctatcg tccggactgg
     1681 ctggcacagt gggaaagagg acatctggta gaagggttgg gagaagcaca ggcctggcaa
     1741 gcgccgttgt ggaaggcgtt ggtggaatat accgacgaac ttgggcaacc gcgctggcac
     1801 cgcgccaatc tctatcagcg ctttatcgaa acgctggagt ccgcgacgac ctgcccgccg
     1861 gggttacctt cgcgcgtctt tatatgcggt atttccgcgt taccgcctgt ttatctccag
     1921 gcgctacagg cgctgggtaa acatattgaa atccatctcc tgtttaccaa cccctgccgt
     1981 tattactggg gcgacattaa agatcccgct tatctggcga aactactgac tcgccagcgc
     2041 cgacacagtt ttgaagatcg cgaattaccg ctatttcgcg acagcgaaaa tgccgggcag
     2101 ctctttaaca gcgatggtga acaggatgtc ggcaacccgc tgctggcttc atggggcaag
     2161 cttgggcgcg actatattta tctcctttct gacctggaga gcagccagga gctggacgct
     2221 tttgtcgatg tgacgccaga taacctgctg cataatattc agtctgacat tctggaactg
     2281 gaaaaccgcg ccgttgctgg tgtgaacatc gaagagtttt cccgtagcga taacaaacgc
     2341 ccgcttgatc cactggatag cagtatcatc ttccacgttt gccatagccc gcagcgtgaa
     2401 gttgaagttt tacacgatcg cctgctggcg atgctggagg aagacccgac acttactccg
     2461 cgcgacatca tcgtgatggt ggctgatatc gacagctaca gtccgtttat tcaggctgtg
     2521 tttggtagcg cacctgcgga tcgttacttg ccttacgcca tttccgaccg tcgggcgcgg
     2581 cagtcgcatc ctgtacttga agcgtttatc agcctgttat cgctgccaga cagccgcttt
     2641 gtgtcggaag acgtgctggc attactggat gtgccggtgc tggcagcgcg gtttgacatc
     2701 accgaagaag ggctgcgtta tttacgtcag tgggtcaacg aatccggaat tcgttggggg
     2761 atagatgacg acaacgttcg cgagctggaa cttcccgcta ccggtcaaca cacctggcgg
     2821 tttggcctga cgcgcatgtt gctgggctac gcgatggaga gcgcgcaggg cgagtggcaa
     2881 tcggttctac cttatgatga atcgagcggc ttaattgcag aactggtggg gcatctgtct
     2941 tcactgctaa tgcagctaaa catctggcgt cgcgggctgg cgcaggagcg tccgctggaa
     3001 gagtggttgc cggtttgtcg cgatatgctc aacgccttct tcctgccgga tgcggaaacc
     3061 gaagcagcga tgacgctgat cgaacaacaa tggcaggcga ttatcgccga aggtttaggt
     3121 gcgcagtatg gcgacgcggt gccgctgtca ctattgcgtg atgaactggc acagcgcctg
     3181 gatcaagaac gtatcagcca gcgttttctc gccggaccgg ttaacatttg tactctgatg
     3241 ccaatgcgtt caattccgtt caaagtggtt tgcctgctgg gaatgaacga cggcgtttat
     3301 ccacgtcagc ttgcgccatt gggctttgat ctgatgagcc agaaaccgaa gcgtggcgac
     3361 cgtagccgtc gcgatgacga ccgctatctg ttcctggaag cgttaatttc cgcgcagcaa
     3421 aaactctata tcagctatat cgggcgttcc attcaggata acagtgaacg tttcccgtcg
     3481 gtactggtgc aggaactgat cgactacatc gggcaaagcc attatctacc gggcgatgaa
     3541 gcgcttaact gtgatgaaag cgaggcaagg gtaaaagcgc atcttacttg ccaccatacc
     3601 cggatgccgt ttgacccgca aaactaccag cccggtaact tacaaagcta tgcgcgcgag
     3661 tggctacctg cggccagcca ggctggtaaa gcacattctg aatttgttca gcccctgccg
     3721 tttaccttgc cggaaactgt gccgctggag acgctacaac gattctgggc acatccggtg
     3781 cgggcgtttt tccagatgcg tttgcaggtg aacttccgta ccgaagacag cgaaattcct
     3841 gacaccgaac cttttattct tgaagggctt agtcgttatc aaatcaacca gcagttatta
     3901 aatgtactgg ttgagcagga tgatgccgaa cgcttgttcc gccgattccg ggcggcaggt
     3961 gatttgccgt atggtgcctt tggtgaaatt ttctgggaaa cgcagtgtca ggagatgcag
     4021 cagcttgccg acagagtcat tgcctgtcgc cagccggggc agagtatgga gattgatctc
     4081 gcctgcaacg gtgtgcaaat aacgggctgg ttgccgcagg tgcagccgga tggcctgttg
     4141 cgctggcgtc cctccttatt aagtgtggcg cagggaatgc aactttggct ggaacacctt
     4201 gtctactgtg ccagcggtgg taatggtgaa agtcgccttt ttctacgcaa agacgtcgag
     4261 tggcgttttc cgccgcttgc agccgaacag gctttgcatt acctctcaca actgattgag
     4321 gggtatcgtg aaggaatgtc cgcgccattg ctggtgttac ctgaaagtgg cggcgcgtgg
     4381 ctaaaaacct gttatgacgc gcaaaacgat gccatgctgg atgacgattc cacgttgcaa
     4441 aaagcccgta cgaaattcct tcaggcttac gaaggcaaca tgatggtgcg tggcgaaggt
     4501 gctgatatct ggtatcaacg gctctggcgg caattaacac cagagacaat ggaggctatc
     4561 gttgaacagt cgcaacgttt cctgttaccg ctgtttcgct ttaatcagtc atgagtattg
     4621 tataaaaatt gcgcaatcta tccgcttgct ttatgatgcg caccagtcac ggactgatgg
     4681 ttatatagac ataggctgac tcgtgcagca caagattaaa ttctggcaga tgatttgcgt
     4741 taacctgttg aatctggcca gaaaataaag ttgattatga ggtccgtgaa tgccccgcag
     4801 cacctggttc aaagcattat tgttgtttgt tgccctttgg gcacccttta gtcaggcaga
     4861 aacgggatgg cagccgattc aggaaaccat ccgtaaaagt gataaagata accgccagta
     4921 tcaggctata cgtctggata acggtatggt ggtcttactg gtttctgatc cgcaggcagt
     4981 taaatcgctc tcggcgctgg tggtgcccgt tgggtcgctg gaagatcccg aggcgtacca
     5041 ggggctggca cattaccttg aacatatgag tctgatgggg tcgaaaaagt atccgcaggc
     5101 tgacagtctg gccgaatatc tcaaaatgca cggcggcagt cacaatgcca gcacggcacc
     5161 gtatcgcacg gctttctatc tggaagtcga gaacgatgcc ttacccggtg cggtagaccg
     5221 cctggccgat gctattgcag aacccttgct cgacaagaaa tacgccgaac gtgagcgtaa
     5281 tgcagtgaat gccgaattaa ccatggcgcg tacgcgtgac gggatgcgca tggcacaggt
     5341 cagcgcagaa accattaacc cggcacaccc cggttcaaag ttttctggtg gtaatctcga
     5401 aactttaagc gacaaaccag gtaatccggt acagcaggcg ctgaaagatt tccacgagaa
     5461 gtactattcc gccaatctga tgaaggcggt tatttacagc aataaaccgt tgccggagtt
     5521 ggcgaaaatg gcggcggaca cctttggtcg cgtgccgaac aaagagagca aaaaaccgga
     5581 aatcaccgtg ccggtagtta ccgacgcgca aaagggcatt atcattcatt acgtcccggc
     5641 gttgccgcgt aaagttctgc gcgttgagtt tcgcatcgat aacaattcag cgaagttccg
     5701 tagtaaaacg gatgaattga ttacctatct gattggtaat cgcagcccag gtacactttc
     5761 tgactggttg caaaagcagg gattagttga gggtattagc gccaattccg atcctatcgt
     5821 caacggcaac agcggcgtat tagcgatctc tgcgtcttta accgataaag gtctggcgaa
     5881 tcgcgatcag gttgtggcgg ccatttttag ctacctcaat ctgttacgtg aaaaagggat
     5941 cgataaacaa tacttcgatg aactggcgaa tgtgctggat atcgacttcc gttatccgtc
     6001 aatcacccgt gatatggatt acgtcgaatg gctggctgat accatgattc gcgttcctgt
     6061 tgagcataca ctggatgcag tcaatattgc cgatcggtac gatgctaaag cagtaaaaga
     6121 acgtctggcg atgatgacgc cgcagaatgc gcgtatctgg tatatcagcc cgaaagagcc
     6181 gcacaataaa acggcttatt ttgtcgatgc gccgtatcaa gtcgataaaa tcagcgaaca
     6241 aactttcgcg gactggcaga aaaaagccgc caatattgcg ctctccttac cggagcttaa
     6301 cccctatatt cctgacgatt tctcgctgat taagtcagag aagaaatacg accatccaga
     6361 gttgattgtt gatgagtcga atctgcgcgt ggtgtatgcg ccaagccgtt attttgccag
     6421 tgagcccaaa gctgatgtca gcctgatttt gcgtaatccg aaagccatgg acagcgcccg
     6481 caatcaggtg atgtttgcgc tcaatgatta tctcgcaggg ctggcgcttg atcagttaag
     6541 caaccaggcg tcggttggtg gcataagttt ttccaccaat gctaacaacg gccttatggt
     6601 taatgccaat ggttacactc agcgcctgcc gcagctgttc caggctctgc tggagggcta
     6661 ctttagctat accgctacgg aagatcagct tgagcaggcg aagtcctggt ataaccagat
     6721 gatggattct gcagaaaagg gcaaagcgtt tgagcaggcg attatgcccg cgcagatgct
     6781 ctcgcaagtg ccgtacttct cgcgagatga acggcgcaaa attttgccct ccattacgtt
     6841 gaaagaggtg ctggcctatc gcgacgcctt aaaatcaggg gctagaccag agtttatggt
     6901 tatcggcaac atgactgaag cccaggcaac aatgctggca cgccatgtgc aaaaacagtt
     6961 gggcgctgat ggttcagagt ggtgtcgtaa caaagatgtc gtggtcgata aaaaacaatc
     7021 cgtcatcttt gaaaaagctg gtaacagcac cgactccgca ctggcagcgg tatttgtacc
     7081 gactggctac gatgaataca ccagctcagc gtatagttct ctgttggggc agatcgtaca
     7141 gccgtggttc tacaatcagt tgcgtaccga agaacagttg ggctatgccg tgttcgcgtt
     7201 cccaatgagc gtagggcgtc agtggggcat gggcttcctt ttgcaaagca atgataaaca
     7261 gccttcattc ttgtgggagc gttacaaggc gtttttccca accgcagagg caaaattgcg
     7321 ggcgatgaag ccagaggagt ttgcgcaaat ccagcaggcg gtaattactc agatgctgca
     7381 ggcaccgcaa acgctcggcg aagaagcatc gaagttaagt aaagatttcg atcgcggcaa
     7441 tatgcgcttc gattcgcgtg ataaaatcgt ggcccagata aaactgctga cgccgcaaaa
     7501 acttgctgat ttcttccatc aggcggtggt cgagccgcaa ggtatggcta ttctgtcgca
     7561 gatttccggc agccagaacg ggaaagccga atatgtgcat cctgaaggct ggaaagtgtg
     7621 ggagaacgtc agcgcgttgc agcaaacaat gcccctgatg agtgaaaaga atgagtgatg
     7681 tcgccgagac actagatcct ttgcgcttgc ccttacaggg cgagcgcctg attgaagcct
     7741 ctgccggcac aggcaaaacc tttacgattg cggcgctcta tttgcgcctg ttacttggac
     7801 taggcggttc tgccgccttt ccccgcccgc tgaccgttga agaactgctg gtggtgacct
     7861 ttaccgaggc tgccacggca gaattgcgtg gtcgtatccg tagcaatatc cacgagttgc
     7921 gcatcgcctg tctgcgtgaa accaccgaca atccactgta caaacgcctg ctggaagaga
     7981 tcgacgataa agcgcaagcc gcgcagtggt tgttgttagc cgagcggcag atggatgaag
     8041 cggcagtctt tactattcac ggcttttgcc agcgcatgct caacctgaat gcctttgaat
     8101 ccggcatgct gtttgagcag cagctgattg aagatgagtc tctgctacac taccaggcct
     8161 gcgccgattt ctggcgtcgc cactgctacc cgctgccgcg tgaaattgcc caggtcgtct
     8221 ttgaaacctg gaaagggccg caggcgttgc tgcgcgatat taatcgttat ctgcaaggcg
     8281 aagcgccggt tatcaaagca ccgccgcccg atgatgaaac gctggcttcc cgccacgcgc
     8341 aaattgtggc gcgtatcgac gccgtaaaac agcagtggcg cgacgcggtg ggtgaactgg
     8401 atgcgctgat cgaatcttct ggtattgatc gacgcaagtt taaccgtagc aatcaggcta
     8461 aatggatcga caagatcagc gcctgggcag aagaagagac caacagttat cagttgccag
     8521 agtcgctgga aaaattctct cagcgtttct tagaagatcg cacgaaagcc gggggggaaa
     8581 ccccgcgaca tccactgttt gaggcgatcg atcaactgct tgcagaacca ttgtcgatcc
     8641 gcgatctggt gatcactcgc gcattggctg agatccgcga aacagtagcg cgtgaaaaac
     8701 gccgccgtgg cgaattgggt tttgatgaca tgttaagtcg gctcgattcc gcgctgcgta
     8761 gtgaaagcgg cgaggtattg gcagcggcga tccgtacgcg attcccggtg gcaatgatcg
     8821 atgaatttca ggataccgac ccccaacagt atcgaatttt tcgccgtatc tggcaccatc
     8881 agccggaaac cgcattgttg ctgattggcg acccgaaaca ggccatatat gcattccggg
     8941 gtgcggatat cttcacttat atgaaggcgc gtagcgaagt tcatgctcac tacaccttag
     9001 acaccaactg gcgttccgca ccgggaatgg tgaacagcgt taacaaactt ttcagccaga
     9061 ctgatgacgc gttcatgttt cgcgaaatac cgtttattcc tgtgaaatca gccgggaaaa
     9121 atcaggcgtt acgttttgtg tttaaaggtg aaacgcagcc tgcgatgaaa atgtggctga
     9181 tggaaggcga aagctgcggc gttggcgact atcaaagtac catggcgcag gtatgtgctg
     9241 cgcaaatccg cgactggtta caggctgggc agcgcgggga agcgttgctg atgaacggcg
     9301 atgatgcacg tccggtacgt gcttcggaca tcagtgtgct ggtgcgcagc cgccaggagg
     9361 ccgcccaggt gcgcgatgcc ttaacgttgc tggaaatccc ttccgtttac ctttcgaacc
     9421 gcgacagtgt ttttgaaact ctggaagcac aggagatgct ttggctgttg caggcggtga
     9481 tgacacccga acgtgagaac accctgcgca gtgcgctggc aacgtcaatg atggggctga
     9541 atgcgctgga tattgaaacg ctgaataatg acgaacatgc gtgggatgcg gtagtcgaag
     9601 agttcgatgg ttatcggcaa atctggcgca aacgtggcgt tatgccgatg ctgcgggcgc
     9661 tgatgtcggc gcgtaacatt gcagaaaact tgctggcaac agcaggcggt gagcggcgtc
     9721 ttaccgatat cttgcatatc agcgaattgc tacaagaagc cggcacgcag ctggaaagtg
     9781 aacatgcgct ggtacgctgg ttatcgcaac atatcctcga gccagacagt aatgcctcca
     9841 gccaacaaat gcgtctcgaa agtgataaac atctcgtgca gattgtcacg atccacaaat
     9901 cgaaagggct ggaatatcca ttggtctggc tgccgtttat caccaatttc cgcgtccagg
     9961 atcaggcgtt ctatcacgat cgccactcgt ttgaggctgt tctggatctt aatgctgcgc
    10021 cagaaagcgt cgacctcgcg gaggccgaac gtctggcgga agatctgcgt ttgctttacg
    10081 tggcgctgac gcgttcggtt tggcattgca gtctcggcgt tgcaccgctg gtgcgccgtc
    10141 gtggcgataa aaaaggtgat accgacgtcc accaaagtgc gctcgggcgt ttgctgcaaa
    10201 aaggggaacc gcaagatgcg gcagggcttc gcacctgtat tgaagcgtta tgcgatgatg
    10261 atattgcctg gcaaacggca caaactggtg ataaccagcc ctggcaggtt aatgatgcat
    10321 taactgcaga actgaatgcg aggacgctac aacgattgcc cggcgataac tggcgtgtca
    10381 ccagctactc cggtttgcag cagcgtggtc acggtatcgc tcaggatctg atgccacggc
    10441 tggatgttga tgccgcgggc gtggtcagcg tcgttgaaga accgacgtta acaccgcatc
    10501 agttcccgcg cggtgcgtca ccggggacat tcttgcacag tttgtttgaa gacctcgatt
    10561 ttacccagcc ggttgacccg aactgggtac aggaaaaact ggagctcggt ggctttgaac
    10621 cgcagtggga accggtgttg actgagtgga tcacaactgt cctcctggca cctctcaatg
    10681 aaacgggtgt tagcctgaat cagctttccg atcgcgataa acaggtggag atggagtttt
    10741 acctgccgat tagtgaaccg cttatcgcca gccagcttga tgcactaatt cgccagtttg
    10801 acccgctatc cgctggctgc ccgccgctgg agttcatgca ggtacgtggc atgttaaaag
    10861 gctttatcga tctggtgttc cgccacgaag ggcgttatta cctgctcgac tataaatcca
    10921 actggttggg tgaagacagt tcggcttaca cccaacaggc tatggcagcg gcaatgcagg
    10981 cacaccgcta tgatctgcaa tatcagcttt ataccctggc gctgcaccgt tatctgcgcc
    11041 atcgtattgc tgattacgac tatgagcgcc actttggcgg cgttatttat ctgttcctgc
    11101 gtggcgttga taaagaacat ccgcaacaag ggatctacgc gacccgaccc aacgccgggt
    11161 tgattgacct gatggatgag atgtttgccg gtatgaccct ggaggaggcg taatgaaatt
    11221 gcaaaagcaa ttactggaag ctgtggagca caaacagcta cgcccactgg acgtgcagtt
    11281 tgccctgacc gtggcgggag atgaacatcc tgccgtcacc ctcgcggcgg cactattaag
    11341 tcatgatgcc ggagagggac acgtttgttt gccgctttca cgactggaaa ataacgaggc
    11401 atcgcatccg ctgttggcga cctgtgtcag tgaaatcggt gagctacaaa attgggaaga
    11461 atgcttgctg gcttcacaag cggtcagccg gggagatgaa ccaacgccga tgatcctctg
    11521 tggcgatcgt ctttatttga atcgcatgtg gtgtaacgag cgcacagtgg cacgcttttt
    11581 caacgaagtg aatcatgcca ttgaggttga tgaagctcta ctggcgcaaa ccctggacaa
    11641 actttttcca gtaagcgatg aaattaactg gcaaaaagtt gcggcggcag tggcgctgac
    11701 acggcggatc tcggtgattt ccggcggtcc tggcaccggt aaaacgacca ccgtagcgaa
    11761 gttactggca gcgttaattc aaatggccga cggcgaacgc tgccgtatcc gtctggctgc
    11821 accaacgggt aaagctgccg cgcgattaac cgaatctctc ggcaaggctt tgcgacaatt
    11881 accgctgacc gatgaacaaa agaaacgcat tccggaagat gccagcactt tgcaccgatt
    11941 gctgggtgcg cagccgggta gccagcgttt acgtcatcat gccggtaacc cgctgcatct
    12001 tgatgtgctg gtggtagatg aagcgtcaat gatcgatctt cctatgatgt cgagactgat
    12061 agacgccttg cccgatcatg cgcgagtgat ctttctcggc gatcgtgatc aactggcctc
    12121 ggttgaggct ggggctgtgc tgggcgatat ctgcgcttat gccaacgcgg gctttacccc
    12181 cgagcgtgcc gggcagctaa gccgcctgac gggaactcac gttccggcag gaactggcac
    12241 agacgcggca tctttgcgcg acagcctctg cctgctgcaa aaaagctatc gtttcggcag
    12301 cgattctggc attggtcagt tagctgcggc gattaaccgt ggtgataaaa cggcagtgaa
    12361 aaccgttttt cagcaggatt ttactgatat cgaaaaacga cttttacaga gcggcgaaga
    12421 ttatattgcg atgcttgagg aagctcttgc gggttacgga cgttatctgg atctgctgca
    12481 agcgcgtgcc gagccgaatt taatcattca ggcgttcaat gagtaccagc ttttgtgcgc
    12541 cctgcgggaa gggccgtttg gcgtggctgg actgaatgag cgaattgagc agtttatgca
    12601 acagaagcgc aaaattcatc gtaatccgca ctctcgttgg tacgaaggcc gaccggtgat
    12661 gattgcccgt aatgacagcg cgcttgggtt gtttaatggc gatatcggta ttgcgctgga
    12721 tcgcgggcag gggacgcgcg tctggtttgc gatgccggac ggcaatatta agtctgtgca
    12781 accgagtcgc ttgccagagc acgaaacgac gtgggcgatg acggtacata aatcgcaggg
    12841 atcggagttc gaccatgcgg cgttgatttt accgaatcaa cgcacgccgg tagtaacgcg
    12901 agagctggtt tacaccgcgg tgacccgcgc gcgtcgccgt ctgtcgctgt atgccgatga
    12961 gcgcatatta agtgcggcaa tcgccactcg tactgagcgg cgcagtggtc tggcggcgtt
    13021 gtttagttcg cgggaataaa cgtaattgcc ggatgcgacg cacgagtgtt acgcatgtcg
    13081 catccgacga ttttcatcgc ttaccccaaa tccgccatca acactttgga tttacgctgg
    13141 tagttgtaca actgcttttt gctctcgggc agtaaatcaa tatccactgg ggtaaatcca
    13201 cgttcctgga accagtgaat actgcgcgtg gtcagcacaa acaatttgct taagccgctc
    13261 tgcttcgcct gagcggcaat gcgttccagc agaacctcgc cccgtgatga actgcggtaa
    13321 tccgggtgaa ctgccacaca ggccatttcc ccaatcttct cttccgggaa cggatagagc
    13381 gctgcgcagg caatagtcgt attatcgcgc tgaataatgg tgaatttgtc gatttccatc
    13441 tccagctgct cgcgagaacg gcgcaccaga ataccttgct gctccagcgg gcgaatcaac
    13501 tccagaatac cgccgatatc gttgattgtt gcgcgacgaa tctgctcggc gctttccatc
    13561 acaatctgcg taccgatacc gtcgcgtgag aacaactctt gcaacagcgc gccatcttcc
    13621 tgataactga ttaaatgaca gcgacgcacg ccgctgcggc aggctttcac tgcgccacgc
    13681 aaaaagcgca ccgtaccgga gttgtaatcg cctttctctt cctgggcttc tacccgtgct
    13741 tgcgcttcgt tagggaaaag ttcggagaca atatcaccgt cgtcattagt gacgccctgg
    13801 gaagagcaaa aaccaatcat tttttcagct ttcagtttga tggccagttg agtggcaatc
    13861 tcttccgagg tcagattaaa gctctcgcca gtgaccgaaa cagcgaccgg ccccattagc
    13921 actattgcac cgctgtccag ttgacgatgg atcgcgtctt catcaatccg ccggatacgc
    13981 ccgctatgac agtaatccac gccgtcatcg acgcccagcg gctgggcaat aataaaatta
    14041 ccactgacga cgttgatatg cgcgccctgc agcggtgtgt tattgagact catcgacagg
    14101 cgagcagtaa tatccagttg caatgttccc gctgcctgct tcaccagttc cagtgttttg
    14161 gcgtcggtca cacgtatatt cttgtgatac agcggttcgt ggtgatgtgc agccagattt
    14221 gcgtcgatct gcggacgtgc gccatagacc accaccagac ggatgccgag gctgtgcaac
    14281 aacccgatat cattaacgat actggagaaa ttctcatgct caatggcttc accgccgagc
    14341 atgatgacaa acgtttttcc ccggtgggta ttgatatagg gaaccgaatg gcggaatccc
    14401 tcgaccagct cggttttacg ttcctttacc acggtacacc tctttgcatg attattcgaa
    14461 attattgtat ttttattctg ttttttcgca gggtgcaagt gtaaatttta tgcgggagcg
    14521 agttttttat cagtaatacc gtgaatataa aaagaatgtt taccgtttta tagatgacag
    14581 attatgcgtc tttcgctaaa gtttccggtc aaattggtcg tttacttgtt acacagctta
    14641 gatttctttt gctcgggaga ggcatgtcag gatccaacac tgcaatcagc cgtcgtcgtt
    14701 tactgcaagg cgcgggtgcc atgtggctat tgagcgtaag tcaggtcagc ctggctgcgg
    14761 tcagccaggt cgtggcggtg cgcgtctggc ctgcgtccag ctacacccgc gtgacggtag
    14821 aatcaaatcg tcagctgaaa tataagcagt tcgcgttgag taatcctgaa cgcgtggtgg
    14881 tggatatcga agatgtaaac ctgaactcgg tgctcaaggg gatggctgcg caaatccgcg
    14941 ctgacgaccc gttcatcaag tcggcgcgcg tcgggcaatt tgacccgcaa accgtacgta
    15001 tggtttttga attaaagcaa aacgtaaaac cgcagctgtt tgcccttgcg ccggtcgccg
    15061 ggtttaaaga gcgtctggtg atggacctct atccggccaa tgcacaggat atgcaggacc
    15121 cgctgctggc gctgctggag gattacaaca aaggcgacct cgaaaagcag gtgccgcctg
    15181 cgcagagtgg accacagcca ggcaaggccg ggcgcgatcg tccgattgtc attatgctcg
    15241 accctggcca cggcggcgaa gactccggcg cggtggggaa atacaaaacg cgcgaaaaag
    15301 acgtggtatt gcaaatagct cgccgtctgc gctctctgat cgagaaagag ggcaatatga
    15361 aggtttacat gacgcgtaat gaagacatct tcattccgtt gcaagtgcgc gtagcaaaag
    15421 cccagaaaca gcgcgctgac ttgtttgttt ctatccatgc cgacgccttt accagtcgcc
    15481 agccgagcgg ttcatcggtg tttgcgctct caaccaaagg cgcaaccagt actgcggcaa
    15541 aatatctggc acaaacccag aacgcctcgg acttgattgg tggcgtaagc aaaagcggtg
    15601 accgctatgt cgaccacacc atgttcgata tggtgcagtc gctgaccatt gccgacagcc
    15661 ttaagtttgg taaagcggtg ctgaataagc tcggtaaaat caacaagctg cataaaaatc
    15721 aagttgaaca ggccgggttt gccgtactaa aggcaccaga tattccctcc attctggtcg
    15781 aaacggcgtt tatcagtaac gttgaggaag agcgtaaact gaaaacggcg actttccagc
    15841 aggaagttgc ggagtctatt cttgcgggaa ttaaagcgta ttttgccgat ggggcgacgc
    15901 tggcgagaag gggatgataa aaaggcgctg aatggcgctt tttttatggg agaccagaaa
    15961 caaaaaaaca cccgttaggg tgttcaataa ttggttgcgg gggccggatt tgaaccgacg
    16021 acct
//
LOCUS       DAZFJL010000106        16014 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZFJL010000106 DAZFJL010000000
VERSION     DAZFJL010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.106"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            45..166
                     /locus_tag="ACHJ8C_003380"
                     /pseudo
     CDS             45..166
                     /locus_tag="ACHJ8C_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            228..926
                     /gene="nleD"
                     /locus_tag="ACHJ8C_003381"
     CDS             228..926
                     /gene="nleD"
                     /locus_tag="ACHJ8C_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector zinc metalloprotease NleD"
                     /protein_id="HFO5553183.1"
                     /translation="MRPTSLNLVLHQSSTSSSMSDTDIESLVKASSVQWIKNNPQLRF
                     QGTDHNIYQQIEAALDKIGSTETGRVLLNAIESISRLKSETVVIHLNSSRLGVMAHRD
                     IDAENHRGTGSDFHCNLNAVEYPCGEGISVVDFHATIVFHELLHVFHNLNGERLKVES
                     SRPESQKYSPLLLEEARTVGLGAFSEEVLSENKFREEIGMPRRTSYPHDSALIHDDNT
                     VSLGFQQVRLHPLL"
     gene            complement(1023..1115)
                     /locus_tag="ACHJ8C_003382"
                     /pseudo
     CDS             complement(1023..1115)
                     /locus_tag="ACHJ8C_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504497.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1347..1403
                     /locus_tag="ACHJ8C_003383"
     CDS             1347..1403
                     /locus_tag="ACHJ8C_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5553184.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            1621..2280
                     /gene="yegL"
                     /locus_tag="ACHJ8C_003384"
     CDS             1621..2280
                     /gene="yegL"
                     /locus_tag="ACHJ8C_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO5553185.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFTIG
                     VQGADMKTLAQISVRQPLSLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            2277..3038
                     /gene="pphC"
                     /locus_tag="ACHJ8C_003385"
     CDS             2277..3038
                     /gene="pphC"
                     /locus_tag="ACHJ8C_003385"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFO5553186.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSSLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALAMWLP"
     gene            3035..4975
                     /gene="yegI"
                     /locus_tag="ACHJ8C_003386"
     CDS             3035..4975
                     /gene="yegI"
                     /locus_tag="ACHJ8C_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO5553187.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTMPSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYYGVPLISDAGNALEMDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDLLRQQLKKCTVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLARVWAMVMASVAPPALQLPLPDHFQPTGRPLPSGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILIPVLAAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRSVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKIAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(4988..6340)
                     /gene="yegD"
                     /locus_tag="ACHJ8C_003387"
     CDS             complement(4988..6340)
                     /gene="yegD"
                     /locus_tag="ACHJ8C_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5553188.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            6474..7322
                     /gene="alkA"
                     /locus_tag="ACHJ8C_003388"
     CDS             6474..7322
                     /gene="alkA"
                     /locus_tag="ACHJ8C_003388"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO5553189.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLHLPGCVDAFEQGVRAILGQLVSVAMAAKLTSRVAQLYGERLDDFPDYVCFPTPQR
                     LAVADPQALKALGMPLKRAEALIHLANSALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            complement(7432..10749)
                     /gene="dgcE"
                     /locus_tag="ACHJ8C_003389"
     CDS             complement(7432..10749)
                     /gene="dgcE"
                     /locus_tag="ACHJ8C_003389"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO5553190.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTIYEPQQAAAHSERAA
                     ISLDEQWRMIKENQLMMIAHGIASPRLPEASNLWLISLKLWSCESEIIDEQTFRRSFS
                     DPALSHALDQRVCHDFFQQAAKAVASKGLSIALPLSVAGLSSATLVNELLEQLENSPL
                     PARLLHLIIPADAIFDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     NAQPLDLLVNRSYFAIN"
     gene            11067..11708
                     /gene="udk"
                     /locus_tag="ACHJ8C_003390"
     CDS             11067..11708
                     /gene="udk"
                     /locus_tag="ACHJ8C_003390"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO5553191.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            11800..12381
                     /gene="dcd"
                     /locus_tag="ACHJ8C_003391"
     CDS             11800..12381
                     /gene="dcd"
                     /locus_tag="ACHJ8C_003391"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO5553192.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            12403..14256
                     /gene="asmA"
                     /locus_tag="ACHJ8C_003392"
     CDS             12403..14256
                     /gene="asmA"
                     /locus_tag="ACHJ8C_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO5553193.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLMA
                     AIEQINWQLQGADLPKQGIQGQGSFQVQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRISFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLAEQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(14324..15907)
                     /gene="yegH"
                     /locus_tag="ACHJ8C_003393"
     CDS             complement(14324..15907)
                     /gene="yegH"
                     /locus_tag="ACHJ8C_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO5553194.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGEMEYEV"
BASE COUNT         3879 a         4046 c         4213 g         3876 t
ORIGIN      
        1 ttttatccag tgtagtggat ttgttgcatg gatggagttg gtaacaaatt gtgtggttgg
       61 caattcttgg cggatcacgg aaatgggcaa tgtcgctgag gactggcaaa tggctatgat
      121 ccgctttatt atcgggtttg gtgaccgcct tgacggtcac ttttaaaaaa atcattttca
      181 cagagtggta aacaggctgc taataagtag cattctcagg agtcctgatg cgccctacgt
      241 ccctcaactt ggtattacat cagtcatcaa cgtcgagctc aatgtcagat acagatatcg
      301 agtctcttgt aaaagcatcg agcgttcaat ggataaaaaa taatccgcaa cttcgtttcc
      361 aggggactga tcataatata tatcagcaga ttgaagcagc actcgataag attggctcta
      421 cagagacagg gcgtgtactc ctgaatgcta ttgaatcaat atcccgactt aaatcagaaa
      481 cagtggtaat acacctcaac tcttccagac taggagttat ggcacataga gatatagatg
      541 ctgagaacca tcgggggact ggttccgatt ttcactgtaa tctgaatgca gttgaatatc
      601 cctgtgggga ggggattagc gtggtggact ttcatgcgac tattgttttt catgagttgc
      661 tccatgtttt ccacaattta aatggggagc gtttgaaagt tgagagttcc cgaccagaat
      721 cacaaaaata ctctccactt ttactcgaag aagccaggac tgttgggttg ggggcttttt
      781 cagaggaggt gctttcagaa aataaattcc gcgaagagat tgggatgccc cgtagaacct
      841 cctacccgca cgactcagct cttattcatg atgacaatac agtgagtctg ggattccaac
      901 aggtaagact gcatccattg ctttagctgt gtttcttgtg ctgaatatat attggtgggg
      961 caaacggagg cacacaaaac tttgcactgg attgcaaggc tttgtgctat tccgatagtg
     1021 gttcaggtgg agtaccccac cttttcatca agccagccca cccaccactg catcatttct
     1081 ctgcgcttat agagatactg agcatggttg taaattcagc aaacgacgca atgtgtttga
     1141 caaaaaatta gcgcaagaag acaaaaatca ccttgcgcta atgctctgtt acaggtcact
     1201 aataccatct aagtagctga ttcatagtga ctgcatatgt tgagttttgt cgcattttgc
     1261 agtctattat ttaatcgcgg attcgtgtaa tgcgatgatt tttaagtgat ttatattatt
     1321 tgtcatcctt taataaggaa agggtcatga tgaagctact catcattgtg gtgctcttag
     1381 tcattagctg gccagcttac taagactacc agggcggggg aaaccccgct ctaccctcac
     1441 tcctgaaatt atgtcactcc gcccttagtg tcaatcgacg gctctccgtt cttgcgaggc
     1501 gaatagcaaa atcttctgcg ccgccgggcc taattctgaa agtttcttcg tttgctctta
     1561 tttcattatt gcaatcggta tgataccgga cctcaataat tctctaagaa aggatttctt
     1621 atgagcgaac aaatcacgtt tgccaccagc gattttgcca gtaacccgga accacgttgc
     1681 ccctgcattt tgctgctgga tgtttctggc tcgatgagcg gcagacccat taatgaactt
     1741 aacaccggat tggttacctt tcgcgatgag ctgcttgccg attcgctggc cttaaaaaga
     1801 gtggaactcg gcattgtgac gttcggcccg gtgcatgtgg aacaaccctt taccagcgcc
     1861 gctaattttt tcccgcccat cctgtttgcc cagggcgata caccaatggg cgctgccatt
     1921 accaaagccc tggatatggt tgaggagcgg aaacgtgaat atcgtgccaa tggtatttcc
     1981 tattatcgtc cgtggatttt cctgatcacc gatggcgcac caaccgatga gtggcaggct
     2041 gccgccaaca aagtgtttca gggggaagaa gataagaagt ttgccttctt taccatcggt
     2101 gttcaggggg cggatatgaa gacgctggcc caaatcagcg tccgtcagcc tttgtctctg
     2161 cagggattac aattccgtga actgtttagc tggttatcca gttcgctgcg ttcggtttcc
     2221 cgctccacgc cgggaacgga agttgtgctg gaagcgccaa aaggctggac gtcagtgtga
     2281 gctggcgact ggtctatgca tcagccgttg gaacgtcgca tattagcgct gatttgcctt
     2341 gccaggatgc ctgccagatg caggttgcct ggctaaatga ccagcagcca ttattgtcgg
     2401 tatttgtcgc tgatggcgca ggcagcgtct cgcagggtgg cgaaggtgcg atgctcgccg
     2461 tcaatgaagc gatggcttat atgtcgcaaa aagtgcaggg cggggaattg gggcttaatg
     2521 atgtcctcgc aacagacatt gtgctgacca ttcgtcagcg actttttgcc gaggcggaag
     2581 ccaaagaatt agcggttcgc gattttgcct gcacgttttt gggattgatt tcatcagcta
     2641 atggcacctt gattatgcag attggcgatg gcggcgtggt ggtagatttg ggtcacggtt
     2701 tgcaactgcc gctcacgccg atggtgggtg aatatgccaa tatgacgcac ttcattaccg
     2761 atgaagatgc cgtttccagt ctggaaactt tcaccagcac tgagcgtgcg cataaagttg
     2821 cagcatttac ggatggtatt cagcgactgg cgttaaatat gctggataat tctccgcatg
     2881 tgcctttctt taccccgttt ttcaatggac tggcgtcagc aacgcaggaa caactcgatt
     2941 tattacctga attgttaaag cagtttttgt ccagcccggc ggtaaacgag cgtacggatg
     3001 atgataaaac gttggcactg gctatgtggt taccatgaaa cccactttat atactgctac
     3061 tggtgagtgc gttacgccag gccgtgaact gggcaaaggc ggcgaaggtg cggtttatga
     3121 tatcgaggag tttgtcgata gcgtcgccaa gatttatcac acgccgccac ccgccttaaa
     3181 acaggacaaa cttgccttta tggctgcgac agctgacgcg cagttgttga attatgtcgc
     3241 ctggccgcag gcaacgcttc acggtggacg aggcggaaaa gttatcggtt ttatgatgcc
     3301 aaaagtttct ggtaaagaac cgattcatat gatctatagc ccggcacatc gtcgccagag
     3361 ttaccctcat tgtgcgtggg attttctact ctatgttgcg cgcaatattg cttcatcttt
     3421 tgctactgtt catgagcacg ggcacgtcgt gggtgacgta aaccagaaca gctttatggt
     3481 aggtcgcgac agcaaagtgg tgttgattga tagcgactcc tttcagatta atgccaatgg
     3541 tacactgcat ttatgcgaag tcggcgtgtc gcattttacg ccgccagagc tgcaaaccat
     3601 gccatcattt gtcggctttg aacgcacaga aaatcacgat aattttggcc ttgcgttgct
     3661 gatatttcac gtcttgtttg gtgggcggca tccttattac ggtgtaccgc ttatctctga
     3721 tgcgggtaat gcgctggaga tggatattgc ccatttccgt tatgcctacg cgtcagataa
     3781 tcagcgacgt ggtttaaaac cgccgccacg atcgattccg ctgtcgatgt taccgggcga
     3841 tgttgaagcc atgtttcagc aggcattcac ggaaagtggc gtggcaaccg ggcgtccgac
     3901 ggcaaaagcg tgggtagcgg cacttgattt actacgccaa cagttaaaga aatgtaccgt
     3961 ttcggcaatg catgtttacc ccgctcattt gactgactgc ccgtggtgtg cgctggataa
     4021 tcaaggcgtt atctatttta ttgatctcgg tgaagaggtc attaccaccg gcggtgattt
     4081 tgtgctggcg agagtctggg cgatggtgat ggcatcagta gcaccgccag cattgcaact
     4141 gccattaccc gatcatttcc aaccgactgg caggccgctt ccttcaggcc tgttacggcg
     4201 cgaatacatc attctgattg agatcgcact gtcagcgtta tcgctgttgc tttgcggcct
     4261 tcaggcagaa ccgcgttata ttattttgat tcctgtgctg gcggctatct ggattattgg
     4321 cagcctgaca agcaaagcgt acaaagcaga aatccagcaa cgccgtgagg catttaatcg
     4381 tgcgaaaatg gactatgacc atttagtcag ccagatccag cagttgggcg ggctggaagg
     4441 ttttatcgcc aaacgggcga tgctcgaaaa aatgaaggac gaaattctcg ggttaccgga
     4501 agaagagaaa cgcgctctgg cagcacttca cgacaccgca agggaacggc agaagcagaa
     4561 gtttctggag ggatttttta ttgatgttgc ctctattccc ggcgttggcc ctgcgcgtaa
     4621 agcggcgtta cggtcctttg gtattgaaac agcagcggat gttacccgac ggagcgttaa
     4681 gcaagtaaaa ggttttggtg atcatctgac ccaggcggtt atcgactgga aagcgagctg
     4741 tgaacgccgt tttgtgttca ggccgaacga agcggtaacg cctgcagaca gacaagcggt
     4801 aatggcaaaa atagccgcca aacgacatcg gctggaatcg gcgctgactg tcggtgcgac
     4861 agagttgcag cgattccgcc ttcatgcgcc agcacggacc atgccgttga tggaaccgct
     4921 acgtcaggcg gcagaaaaac tggctcaggc gcaggcagat ttaagccgtt gctgagccat
     4981 ttttcaatta acgaaacacc acttccgccc agcgtgccag cccggcggtg acggagccaa
     5041 agtcatcgcc gcctgcaatc ggaatgcccg gcaactgttc tgccagcgct tttttaatca
     5101 gcggcgaacg ggcgctaccg ccggtcaggt agataacgtc cggtttttcc tgggcgttat
     5161 ccagcgccag ttgcacctgt tccagaatcc gcgccagtgg ctggctgagg gcgctttcca
     5221 gcccttgctg gctaatcagc gtagccagtt catcgctgat aaacggcagt gaggcgcggg
     5281 tttccgctac acttgaaagg gcgattttgc tctcttctgc gctacgcacc aggcgatagc
     5341 ttaaacgctg acgccagact ttctgtaaca gggccacttt ttccggttca cgggcatcgc
     5401 gtaccagatc gttaagcaga cgaccgttag cactactgta gaaatcactt tgcgcaggaa
     5461 cgtcgttgat ggcaaccgca ttccaccacg gcagaatcgg cagggcgatg cctttttcgg
     5521 tttcgccacc catgcccagt aacggcatca ggttcttaaa cgccagtgcg atatccagat
     5581 cgttaccacc aatacggcaa ccactatgac ccagcaggct ggcttcacga tcgagacgcg
     5641 aacgccattg cggtcccatc agcagcaatg aacagtcagt cgtaccaccg ccgatatcca
     5701 ctaccagcac tcgtttttct tcctgcaagg tggcttcgta atccagccca gccgcgaccg
     5761 gctcgtactg gaataccaca tccttaaacc cggcacgctt cgccgcgcgt tccagaatcc
     5821 cttgcgcctg ggtgtttgct tcatcaccgc ccagcccctg gaagttgatc gggcgaccaa
     5881 tcaccgcctg agtaattgcc tccggcagct gcgcctgcgc ctgttggcga atgtgcaaca
     5941 tcattgcgca gaccagatcc tcaaacagcg ctacctgctg cggttttaag ccgctggcac
     6001 cgaggaacga ttttggtgat ttcacaaacc acacttcttc tggatcatca atgtactgtg
     6061 ccagtgagga aagaccgaac tgcacgcttt tcgccgtaac atcgatatct tcttcgcggt
     6121 tataacgaat cgcccgacgc aacagcgcct gcgtttcatc gtcgtctgcc ggaacatcat
     6181 gatggcggta cagccattcg cttaccgctt cacgcgttgg cgcgcaaagc attgaaggca
     6241 gcagcgtgct gtcgttttcc atttttagca aatgcggttt accgtcgcgc atgaccgcca
     6301 ctgaacagtt tgctgtaccg taatcaaaac caataaacac gaaataatcc ccatgccggt
     6361 gaagaagggg cgtgacttta gcgaaatgtt gccgtcgcga caaccggaat atgaaagcaa
     6421 agcgtaacgt ctgaataacg tttatgctga aagcggatta acaaggagat gcgatgtata
     6481 ccctgaactg gcagccgccg tatgactggt cgtggatgtt gggatttctc gccgcccgtg
     6541 cggtgagcgg cgtggaaacg gtcgcggaca gttattatgc ccgtagtctg gcggtaggcg
     6601 aatatcgcgg cgtggtgact gctattccgg atatagcccg ccatactctg cacataaatt
     6661 taagtgcagg tttagaacct gttgccgctg agtgtctggc gaaaatgagc cgcctgtttg
     6721 atctgcaatg taacccgcag attgttaacg gtgcgttggg caagttaggc gcggcgcggc
     6781 caggattgca tttacctggt tgtgttgacg cttttgagca gggcgtgcgg gcgattttag
     6841 gccaactggt gagcgtggcg atggcggcaa aattgacctc cagagtggca cagctttatg
     6901 gcgaacggct ggatgatttt ccggattatg tctgcttccc cacgcctcag cggctggcag
     6961 tagccgaccc gcaggcatta aaagcgttag gtatgccgtt gaaacgggca gaggcgctga
     7021 ttcatctggc aaattcggcg ctggagggca ccttaccaat gacaataccg ggcgacgtag
     7081 aacaagcgat gaaaacgctg caaacctttc cgggtatcgg gcgctggacg gcaaattatt
     7141 ttgctttgcg tggctggcag gcgaaagatg tttttctgcc ggatgattat ctgattaaac
     7201 agcgatttcc gggaatgacg ccggcacaaa tccgccgtta tgccgagcgc tggaaaccct
     7261 ggcgttctta tgcgctgttg catatctggt atacggaagg ctggcaacca gacgaagcat
     7321 gacgttggtc gccggatgcg gtgcgagcgc cttatccggc ctggggaatg agcgatttgt
     7381 aggcctgata agacgcgtta gcgtcgcatc aggcaatggg caaacgctcc cttagttaat
     7441 cgcgaaataa cttctattca ccagcaaatc cagcggttgg gcattggcaa tcacatcacc
     7501 ataaatcaga tcgacgccga tgcccgaaag cgtatccatc actaagggta aaacgactgg
     7561 cccggcgata gtcttcatcc cgagccgctg agcgtgcccc tgaatgatcg taatcagcat
     7621 ctcatccatc aaattaccct gcacgttggc gcataactca ccatcaagta gcaggtaatc
     7681 tgccatattt gctttcagcg agttgaagat ttgcagatct cggcccacct gactgaatac
     7741 gatccgacat cccgccagcc gcagtttttg cacgctttct gcgtgatcga aaatcgcgtc
     7801 tgccggaata atcagatgta ataaccgtgc tggtagaggg ctattttcca gctgctcaag
     7861 cagttcattc accagcgtgg cgctactcaa accagcaacg gaaaggggaa gggcgatgct
     7921 taagccttta ctggcaaccg cttttgcggc ctgctggaaa aaatcgtggc atacccgttg
     7981 gtcaagagca tggctaagtg ccggatcgct gaagctacga cgaaatgttt gttcatcaat
     8041 aatctcgctt tcgcaactcc agagcttaag tgaaatcagc cacaaattac ttgcttccgg
     8101 gagccgtggc gaagcgatgc cgtgggcgat catcatcaac tgattctctt taatcatccg
     8161 ccactgttca tcaagcgata tcgccgcccg ttcgctatgt gcggcagctt gctgcggttc
     8221 gtaaatcgtc acccggcccc ggccaccatt tttggaggca taacaggcga tatcagcctg
     8281 cgacatcact tcagctgcct gatgattgtt gtcatcaatc aaggtaatcc cggcactggc
     8341 acctacccga tgcacacgtc cttcccatat aaagtgatag tcattcacgg cactgataat
     8401 gcgtgtagcg ataaaacgag cgctttcaac attgcagtct ggcagtagca gaccaaattc
     8461 atcaccaccg agccgcgcca gcacgtcgct ggagcgcagc atactcagca tcaacgacgc
     8521 cagttcgcgc agtaaagcgt cgcccgccgc atgcccggcg ctgtcattca ccgctttaaa
     8581 gcgatcaaga tcgataaaca ccagggcatg tcgctggtgt gtactgttta ccgtttgcag
     8641 caggatacgc agttgcttct caaaactggc gcgattggcg agatgcgtca gtgcatcatg
     8701 ggaggcgctg tagctcagct ggcgcagcat tttgcgtgat tcggtgacgt cctgaatcac
     8761 cagtaccgag cctatattgc tgccgtccag agtacttaac ggcgtaatac tgtaatgcac
     8821 gtcgtaactg ccgccgcttc ggcagtgcaa caccacatct tgttcaatcg cggaacgtga
     8881 ggtgtcagca ctataaatgt tctccattaa tggtccgttg tcgccaaaag taatatgcaa
     8941 caccgtcagg agcggaacac ctaacgcttc ttcctgcgtc cagccgctca tcttctccgc
     9001 cactggattc ataaaggtaa ttttcattgc catatcaata cagaccacgg cttcgccgat
     9061 ggagtccagc gtaatgtgca ggcgctcttt ttcctgaaac aatgcctcgt taagctgttt
     9121 aacctccgtc atatccatat taatgccgag caggcgttcg acttcacctt ctttattcag
     9181 tacccggtta gcgagggcgc ggatatggcg aatgccgtct ttcacggtaa tgcgaaattc
     9241 cagtttaaag ggagagcgcg attgcaatga atcgcgaatc actttttcgg cgtgctggcg
     9301 atcttccggc agcacgcact cgtaccacac ctgccagttc ggtttgatat gcggaggaat
     9361 ttcatacagc tcgaacatcc gcttatccca gctaaaaata ttcggcttca gctcccactc
     9421 ccagatgcca atcccgcccg cttcgttggc cagcgtgatg cgctccatca gttgctggtt
     9481 cacctgttcg gtgcgtttta gctcgttaat gtcttcaatc tgagcgataa aatagagcgg
     9541 cgtgccatcc gtgtggcgca ccagtgagac ggcaagcaac gcccagacaa catcgccatt
     9601 gcggttgtag tagcgttttt ccattgaata ggtgtttatt tcaccgctta tcagcttttc
     9661 aacctgttgc agatctttat tgagatcctc cggccaggtc agttgctgaa aggtgagtcc
     9721 gcgcagctct tcctgactgt aaccgagaaa ctggcagagc gctttgttgg attgcagcca
     9781 ttgtccctcg gtgcccacta atgccatgcc gatggcggaa tattccatcg cgttccgaaa
     9841 gcgggtttcg ctttcggaaa tgtgtttgcg ttccgcacga aaggcataca tcaccatcgt
     9901 catgatgttg gcgggcagca ggatcagcaa aaacggtagc cacggcatat gactcatcag
     9961 gtacgtacgc ggcgtagcaa gcagggaggg atccgcggcc atcatcagcg ataccatcat
    10021 cactgtggta aggaagatca aaaaggcttc cattcgtggc aggcgcacgg cgctccacat
    10081 caacagcaca ataatgaaag taaaaggcca cggcagatac agcatcgaaa gccagcttaa
    10141 cgtcagcgtg atggctaacg tgagcagcga ctcaaaaagc aaccttgggt tgcgatggcg
    10201 cagtagatag tgtggtttaa ataacaatcc cagcggcacc agtgccagag cgccgatgga
    10261 ttctgacagt acccatatca aaaatgccct gagaggatcg tctccgggcg tcagcaggac
    10321 aaccagaaca ccccctaaca gaggtggaac aatggcgctg ccgagtgcca gacgcagcca
    10381 gtcagccaga ttttgcaagg ggttatacca cggcagcaat ttacgtagca gcactgcccc
    10441 gaccacggct tcaacaatat tgatggtcgt ccaggtcatg ttcagcgagc tggtggaaaa
    10501 aagcaggatg gatgcggcga tatttcccag cgagcagctc agcgcaattc ccggccacat
    10561 gcgcccggca tggcgataaa acgccaccat catgatggag gtcgggaacc acagtggggc
    10621 gagttgggtg ccaaactgcg aaagctcaag tgagaaaagg gtgaagataa acgagactaa
    10681 acctaaactg accaggtgaa gcagcgggtg gggcagggca attaatacat gctgtgattg
    10741 tttgctcatt accgctttat ccgcaaggcc aggtcgccag tattcgtttc tgtgtttatg
    10801 ctagtacaaa agttttacat tttatatgcg aattgctcat aaagtgacgt aaaggcggat
    10861 aatttgcgca actgcgtata acatttttta ccttgtataa aactgatcaa cgtaatttgc
    10921 caccgggaat tcttcgtcaa aaattaactc tgagcgcgcg gcaacgctat tcgactggta
    10981 tcaggcggat gaaatcccta taattgccgc gtttggcgct tcgtcgcccc cttcctaaca
    11041 tccaggttaa tcaggtcgct aaatatatga ctgatcagtc tcatcagtgc gtcattatcg
    11101 gtatcgctgg cgcatcggct tccggcaaga gtcttattgc cagtaccctt tatcgtgaat
    11161 tacgtgagca agtcggtgat gaacacatcg gcgtaattcc cgaagactgc tattacaaag
    11221 atcaaagcca tctgtcgatg gaagaacgcg ttaagaccaa ctacgaccat cccagcgcga
    11281 tggatcacag cctgctgctt gagcatttac aagcgttgaa acgcggctcg gcaattgacc
    11341 tgccggttta cagctatgtt gaacatacgc gtatgaaaga aacggtgacg gttgagccga
    11401 agaaggtcat cattctcgaa ggcattttgt tgctgacgga tgcgcgtttg cgtgacgaac
    11461 ttaacttctc cattttcgtt gataccccgc tggatatctg cctgatgcgc cgcatcaagc
    11521 gtgacgttaa cgagcgtggg cgttcaatgg attcagtgat ggcgcaatat caaaaaaccg
    11581 tgcgcccgat gtttctgcaa ttcattgagc cttctaaaca atatgcggac attatcgtgc
    11641 cgcgcggcgg gaaaaaccgc atcgcgatcg atatattgaa agcgaaaata agtcagttct
    11701 ttgaataagc ttgataaatt gtgtaccgtt cagtgataac ctggtatgcc cttgacgtaa
    11761 ttggcgttaa gggagtgatg cacgaaagga gaaaatgcca tgcgtctgtg tgaccgagat
    11821 attgaagcct ggcttgatga aggccgtttg tcgatcaacc cacgtccgcc agtggagcgt
    11881 attaacggcg cgacggtgga tgtgcgcctg ggcaataaat ttcgtacctt ccgtggtcac
    11941 acggcagcgt ttatcgatct gagcggtccc aaagatgaag tgagcgccgc gcttgaccgc
    12001 gtgatgagcg atgaaatcgt gctcgacgag ggcgaggcgt tttatcttca cccaggagag
    12061 ctggcgctgg cggtgacgct ggagtcggtg acgctgccag ccgatctggt gggctggctg
    12121 gacgggcgtt cttcactggc gcgtctgggg ctgatggtgc acgtcaccgc gcaccgtatc
    12181 gatccgggct ggtctggctg cattgtgctg gagttttaca actccggtaa gctgccgctg
    12241 gcgctgcgtc cgggcatgtt aattggtgcg ctgagctttg agccgctctc cggcccggcg
    12301 gcgcgacctt acaaccgccg tgaagatgcg aaatatcgca accagcaggg cgcggtagcc
    12361 agccgaatcg ataaagacta attcaggccc attgaggata ccatgagacg atttctgacg
    12421 acgctgatga tactcctggt cgtgctggtg gccgggttat ctgcgttagt gttgctggtg
    12481 aatccgaatg atttccgcga ctatatggtc aagcaagttg ctgcacgtag cggttatcaa
    12541 ttgcagctcg acgggccact gcgttggcat gtctggccgc agcttagtat cctctccggg
    12601 cgaatgtctc tcaccgccca gggcgcaagc cagccactgg ttcgcgccga caacatgcgt
    12661 ctggacgtgg cgcttttacc actactgagt catcaactga gcgttaagca ggtgatgcta
    12721 aagggggcag tgatccaact gacgccgcag acggaagctg tgcgcagtga agacgctccg
    12781 gttgcaccgc gcgacaatac cttgccggat ctgtcagacg atcgcggatg gtcgtttgat
    12841 atatccagtc ttaaggtggc ggacagcgtt ctggtgttcc agcatgaaga tgacgagcag
    12901 gtgacaatcc gcaatatccg cctgcaaatg gaacaagatc cccaacatcg tggctcattt
    12961 gagttctccg ggcgggttaa tcgcgatcag cgcgatctca cgatatccct taacggtacg
    13021 gtggatgctt ctgattatcc gcatgattta atggcggcta ttgaacaaat taactggcag
    13081 ttgcagggtg ccgatttacc aaaacaaggt attcaggggc aggggagttt ccaggtccag
    13141 tggcaggagt cacataaacg cctttcattt aaccaaatta gtttgaccgc caatgacagt
    13201 acgctgagcg ggcaagcgca ggtcacgttg acagagaaac cggaatggca gctgagactg
    13261 caattcccgc aactgaatct tgacaacctc atccccctta atgaaacagc gaatggtgag
    13321 aacggtgccg cgcagcaagg gcagagccaa tcaacgttgc cgcgcccggt catttcttcg
    13381 cgtattgatg aaccggccta tcagggactg caaggcttta cggctgatat tttgttgcag
    13441 gccagtaacg tgcgctggcg cggaatgaat tttacagatg ttgccacgca aatgaccaac
    13501 aagtcgggtt tgctggaaat tactcaactg cagggcaaac ttaacggtgg acaggtttca
    13561 ctgccgggca cgctggacgc gacatcaata aatccgcgga taagtttcca gccacggctg
    13621 gaaaacgttg agattggcac cattctgaag gcgtttaact atccgatttc gttgaccgga
    13681 aagatgtcac tggctggtga tttctccggt gctgacatag atgccgacgc attccgccac
    13741 aactggcaag gacaggcaca tgtcgaaatg accgacacac gcatggaagg gatgaacttc
    13801 cagcagatga ttcagcaagc ggtagaacgt aatggtggtg atgtgaaggc cgctgaaaac
    13861 ttcgataacg tgacgcgtct tgaccgcttt accaccgatt tgacgttgaa ggatggcgtc
    13921 gtgacgttaa acgacatgca aggtcaatcg cctgtgctgg cgctgacagg ggaaggcatg
    13981 ttgaatctgg cggagcaaac ctgtgacacc cagtttgata ttcgggtcgt gggtggctgg
    14041 aacggggaaa gcaaactgat tgatttcctg aaagaaacgc cagtaccgct gcgggtttat
    14101 ggcaactggc agcaacttaa ttacagcctg caagtggatc agttactgcg taaacatcta
    14161 caggatgaag caaaacgccg tcttaacgac tgggccgagc ggaataaaga ttcccgcaat
    14221 ggcaaagatg tgaagaagtt gctggagaag atgtaggtta ccaggtgacc tgtcctgtag
    14281 gcctgataag acgcgtaaag cgtcgcatca ggcctacatg ccatcacacc tcgtactcca
    14341 tctcgccatc cttatgcagc ggaataatct gcaccttctg cacgcgatgg ctttccacct
    14401 gcaacgtttt aagcaaataa tcccccacct gaacttcttc gccaggtttt ggaatacgct
    14461 gcaaatattc catcaatagc ccggcaatgg tgtgatattc acgtttttca tccagcggca
    14521 gcggcacata ttgcaccaga tcttccagcg gcatatgacc attcgccgtc caggaaccgt
    14581 ccgcattctt ctgaatatca tggcgggcgt cgatctcttc cacttcattc ggtaagttac
    14641 cggcaatggt ttcagtgacg tcacttaatg tcacaatccc ttccaccgag ccaaactcat
    14701 ccaccacaaa agcaaagtgt gtgcgggcat tacggaactg ctccagggca ggtaacaacg
    14761 gcaaggtttc cgggaacacc agcggctgac gaatcaacac ccgcaggttg agcggttcgc
    14821 cgcgcagtga ctgttgtaac aggtcgataa cgtgaacaac accgagcaaa tcttctgcgt
    14881 catcgccatc agtaacaaca agccgcgtat gctgatttcg ctccagtaac tggcggatct
    14941 cgtcttccgg cgcgttgaga tcgatatgct caatatcgtg gcgcgacgtc ataatgctgc
    15001 tgacggtacg ctggttaaga ttaagtaccc gctcaatcat ccgccgttcc tgcggattaa
    15061 agatctgctg gttaccatga tccaccagca tggacgcggt ttcggcgtcc agttccgcat
    15121 cttctttttg cccgctaagc aggcgcatta ccgcttcggt agtccgctgg cgcagtgtct
    15181 ggttagcgga aagaaaacgt cgccggttaa agatagccaa ctgattgagc gcctcgatca
    15241 tcaccgagaa accaatggca gcgtacaggt agcctttcgg aatgacgaag ccgaaacctt
    15301 ccgccaccag gctaaagcca atcatcaaca ggaagcttaa gcagagaata acgatcgtcg
    15361 ggtgactgtt aacgaactgg gttaacggct tgctggccat caacatcagg ctgatagcga
    15421 taaccacggc ggccatcatg accagtaaat ggtcgaccat cccgacggcg gtaatcaccg
    15481 agtcgagtga gaagatggcg tccagtacca caatttgtgt caccaccccc cagaacttcg
    15541 cacctttgcg ttgggtgggg ttattgctgt ctttcccttc cagccgttcg ttcagctcca
    15601 tcgtggcttt gaacaacagg aagaaaccac caaacagcat gattaaatcg cgggcgctaa
    15661 aggtaaacga gcggaagctg aacagcggtt gagtcagggt gaccagccag gagattgacg
    15721 ccagcagtaa caggcgcatt aacatcgcca gcaacagccc ggtaacccgt gcgcggtcgc
    15781 gctgcttagg cggtagtttt tcggcgagga tggcaataaa gaccaggtta tcaatgccga
    15841 ggaccagttc gatcacaatc agcgtgatta acccagccca gatagacgga tcggcaatcc
    15901 attccatagt aagtacaaaa cctattcgtt gtatgacaaa tgtcacaatt cgatcatgga
    15961 taaatcgggg caaaaatgca atgttgcagc actatttatc tcatattatt tgag
//
LOCUS       DAZFJL010000107        15876 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFJL010000107 DAZFJL010000000
VERSION     DAZFJL010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.107"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..222)
                     /locus_tag="ACHJ8C_003394"
     CDS             complement(<1..222)
                     /locus_tag="ACHJ8C_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553195.1"
                     /translation="MSRSISEEFKDQKINDLLEDYIALEKQIEKTANLIINAIDETPA
                     RLDAVLSDKVNKIIQSSIDINKDINIAKSD"
     gene            complement(269..970)
                     /gene="stbB"
                     /locus_tag="ACHJ8C_003395"
     CDS             complement(269..970)
                     /gene="stbB"
                     /locus_tag="ACHJ8C_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StbB family protein"
                     /protein_id="HFO5553196.1"
                     /translation="MYLKLAIVNNSGNVGKSTIGQTLLKPRLEGSEIIKVETLNTDGT
                     NDEKLAAKEFDEIIKRIDDSECTIIDVGSSNIEQFLVQMNEYQGSHDLIDYFIVPVVR
                     EAKQQIDSIQTITTLMAMGVEQDRFKVIFNLAEKDTPINKQYSVFLADDVCKEIVGEN
                     PVVVYHNNIFNILNKSGLQYDDVYNDNRDFRSLIRSASSKEERQDLSNLRAVKMLMNG
                     FNSDLDVAFKNLNLV"
     gene            complement(976..1455)
                     /locus_tag="ACHJ8C_003396"
     CDS             complement(976..1455)
                     /locus_tag="ACHJ8C_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553197.1"
                     /translation="MNNGENKLLGSLLAQKVKRSKTGRIRERFAEIEEAQQQGIRNID
                     IVNALNDEGFDLTLKTFENILHRIRKERAEKKDVSHLLSNKEKTYQKAITIEDKNRKT
                     KQDNDILNAYLPVCFNNAKIAQQAIDNNVSIETIKSWNCANFVQVSNTLGNYIRNKR"
     gene            1785..2126
                     /gene="mobC"
                     /locus_tag="ACHJ8C_003397"
     CDS             1785..2126
                     /gene="mobC"
                     /locus_tag="ACHJ8C_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFO5553198.1"
                     /translation="MKKKTNKNVHVTFRLTEEEYAPFDRAIKELNISKSEFFRLLTIG
                     KINTYASDKRNIPEYKRCLSQLSWAGNNINQIAHRLNSDHLKGIISESLYKKVLNGLI
                     GIRDRLQEIAK"
     gene            2123..6334
                     /locus_tag="ACHJ8C_003398"
     CDS             2123..6334
                     /locus_tag="ACHJ8C_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFO5553199.1"
                     /translation="MIVRYGGGNDGIVDYLINGRKAERQYTRDELDHRVVLDGDLQTT
                     DKIIDSIENKSQERYLHITLSFHESHVSNEVLKAIVDDYKKLLMNAYHPDEYSFYAEA
                     HLPKIRHIQDNSTGELVERKPHIHIVIPKVNLITEKFLNPVGDVTKGHTIEQLDAIQE
                     FINNKYNLDSPKDYPRKDADYGKIISRVKGDLYKEHHSELKGELLSRIENEKIENYSV
                     FKDIVAEYGELRIRNAGKTNEYLAVKLPGDKKYTNLKSPLFRQNYIETRTLTLEKPTH
                     KEIEKRLNTWLNKTSYEIKHIFNQAEKTRELYKTLSPSQQIDFLQERIKEYDSREKLN
                     ERNSQQTSGRAGGYKSCPKKFARIRQSEATVGLSRMPQRGMVYGINGFTRPDSVSVLS
                     DISQRDLAEQLSQREHPGQDVRRDYDRQFTESGIKSLERSSFLCETMFQTLNEAAEKN
                     EIATMAEIRRNIDPVRFLSSAAERFNIIPAQHKIRTAKDGSPRFSVGNRNMNASDFLT
                     KHINLAWKDAKSFLLEVYSQQLENTPYTRYPTYRRLTYHEARERLNSLNLSEKTLRNT
                     IRFERGKLYNDLREMRRELKLIPREQRDIAVGVIVYKKLTTLERLSELDTEGRHIIRQ
                     YHADWHKDKDEMKALERLKSYLNFDEINAISADEPELSLQKAVDSQRRLEEAKKVNSK
                     LKDLVMDKQDSRIVYRDQESEKPVFTDKGNFVVAGKNPSKEEIGIMLEYSREKFGGVL
                     KLTGSEDFKKMCAEVAAEQDMKIILRPEQYQQMMLELKAELQGNKFEQVETQENSQES
                     ESRIEKGDALKEQATEQAQATEQTQATEQAQATEQTQATEQAQATEQAQATEQAQATS
                     SYDPGVITRANTLDSQMLSKGTNGEFGYLKSLDSDENEIWEVLGHVPGDSDDIFSVAS
                     FDNENDAKEFCNIVNELGIDRTQALIQEQLTTQHDQATAQVHNKQEIYCINFSRFHDL
                     NEGIVFHSKDAAIQCYEESKSSAIEKYESNYLNGDGFDTVVLMSKTVSTDELSSYPEG
                     ALVTFDRPFEIIANSYEEYRTPVYAVSFSKDEFSEDIKTFESLHDASEYKNKMLQEHG
                     LNQDDILITPVTREEIAFKGIKDAVNDANMAVMEQAGDSSRESPEEILASISANEHMI
                     SGLENFLVKDRSQFSSCNGDIVVEAEITRGEGGLYRLAVAGKHGLERGDAVARVDVTE
                     QQFAAITGKTPSEVLTGDQTSARVPVITGIHFSTRAIENVNKLEQQKDYVYFSTHEGL
                     NAEIKDFSSLKDAIEWGRVECELHDLNKRDTVIYRVESEHISQGIDAVMKNAERVERH
                     EIEKAQGRDCTPEDGKILEAIDRFEDKFRGEGLKFEREKAESDLLNHGFTREMAEDAL
                     GKQFVQAREDHQESQQQRDDSQDMH"
     gene            complement(6377..6817)
                     /locus_tag="ACHJ8C_003399"
     CDS             complement(6377..6817)
                     /locus_tag="ACHJ8C_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553200.1"
                     /translation="MKEYEKTALIYSLKESNTLVLRIILISILVVGVISGAIYLYNLA
                     ISLFDSNPTSVQFTFCGVGCFIALLLQWISNVDANYRERIGEQPSTIKNIVQYLFAAI
                     ITISFITMALTAILVLKDSSNIWLNITFIALLLSIFVAIISVWG"
     gene            complement(7062..7505)
                     /locus_tag="ACHJ8C_003400"
     CDS             complement(7062..7505)
                     /locus_tag="ACHJ8C_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000465834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553201.1"
                     /translation="MFFLYETYNFFYYLIKLIVIQPQYICVYMIFFFFNAGIAYSITN
                     DIEDQVCRWLLFVSMLHALMIPPAVIMPLIMPPKEILQETEKRQELHESIPKTCKLKA
                     LDAQQGGLFGVDKDEWVFPDNKSFYLPEKCRPENRITELAMMKEG"
     gene            complement(7651..7857)
                     /gene="hha"
                     /locus_tag="ACHJ8C_003401"
     CDS             complement(7651..7857)
                     /gene="hha"
                     /locus_tag="ACHJ8C_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO5553202.1"
                     /translation="MKTKQDWLFQLRRCSTLDTLEKIIEKNQNCLPPSELESFNAAAD
                     HRLAELTMNKLYDKVPPSVWKYVY"
     gene            complement(7976..8314)
                     /locus_tag="ACHJ8C_003402"
     CDS             complement(7976..8314)
                     /locus_tag="ACHJ8C_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553203.1"
                     /translation="MKFIILKKDKDSTIARNDSVFPVNNFFVVLTTVSSVANSFGIVS
                     SLAKSIGISFPTGNVIETCFFGMFSLLLAAFSFVSTMGLANAVPPIKNVFPLLFFVLV
                     LITNYNIYFR"
     gene            complement(8789..10945)
                     /locus_tag="ACHJ8C_003403"
     CDS             complement(8789..10945)
                     /locus_tag="ACHJ8C_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFO5553204.1"
                     /translation="MKLFIAEKPAVANDIVKALGGNFTRHDGWFESDNTIVTNCFGHI
                     IESQPPENYNPEYKEWKIETLPLRLYPVKYQPVESAAKQVKTIIELIRRADVTEIIHA
                     GDPDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANPKNNRDFRGLYLKAL
                     ARSVADAIYGLSMTRAYTIPAKTKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFY
                     YTMTGHFQRGADVIRANWKPGEFAPLTDRKLLDKTWANGTATSLAGKPATVEAAATDD
                     KKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQF
                     SEAPQIIDALKSVFDQPMDIDTTRKSKAFNSAKVTAHTAIIPTVSVPDVNALSTDERN
                     VYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGVENAGDDE
                     AEVENDDSAFDLLCKIRTGETVTTKEVVVNEKKTTPPPLFTEATLLAALVRVADFVTD
                     PVIKKLLKDKDRDKKDEHGGIGTPATRASILETLKKRNYITLEKGKLIPTDTGYALID
                     ALPDIAVNPDMTALWAEKQTLIENGEMTIEQFVDELYNDLIPMISNANSAEIKVSPSA
                     PSGQSQRLSSPCPSCGKQIVIRPKSYSCTGCEFKIWNEFSGKKITQAQAEKLIKSGKT
                     DLIKGFKKKSGGTYDAVLVLEDKKTGKLGFPARAKK"
     gene            complement(10950..11525)
                     /locus_tag="ACHJ8C_003404"
     CDS             complement(10950..11525)
                     /locus_tag="ACHJ8C_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO5553205.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKENPVKELPDIDESAHPF
                     HVCYGGYLLNPGVSDSDISRKIKDQTGFWLAAIDDTRMDCHSIAYYDIHTLPLISCGH
                     QKIVPFAALIKADECIISKISSYSGFAVTAFLRIKDWDIATNILNREGIFAFNGCEHR
                     FRQPVSEDNWQQAVSEERAIRCAKRLIQCKG"
     gene            complement(11695..13371)
                     /locus_tag="ACHJ8C_003405"
     CDS             complement(11695..13371)
                     /locus_tag="ACHJ8C_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HFO5553206.1"
                     /translation="MYVINQQTRDNNILTLHDVFKIYQNTGGLRLVKTEENEKYISII
                     ICGVFRIRKNKKNNKITLWGYKLRDKHTGVWTRRNSFPGKIFLFWSKKSAYDMDDWLK
                     YAISYIIKSLIEAGYSIEDKLYLFRVGDEEEHSESRYVSTLYPLSVYNSYSYGRYGII
                     HCLGKTASFNYPDNVQYISRHDLLEEIKNKIYHRALKIIGVDNELNASKALSKAIWIM
                     VDKKIFAQYSRASHCSIAYNSIRQALFEDYLDFSKRIHILDDIDFCGLWPMVGLLRQQ
                     HKKGQFMLSGKTKELYERLCFPYELSYGEFRSLRHVSLSLVYALIYNHDNASFRLTVR
                     LLRHPLIKNYPVRAIYWIIDYISIRAYPEKENDIYRICSKWLEYHRDLFKNIGFYDRN
                     TESRQTSRWEMETNQLCHAIDWLLAEERLIHKNQEWPSFWRLSDEWTRQVKNNVIPAT
                     QKWKGTGINWQKIDNCVNELITFDALCQEGQEMEHCVASYADWCASGEYIAISVLMDN
                     ERATLGLSRKEHDLTYQFDQMRGIRNQAVSRNMLIKGRHILKIINSSLKG"
     gene            complement(13466..13998)
                     /locus_tag="ACHJ8C_003406"
                     /pseudo
     CDS             complement(13466..13998)
                     /locus_tag="ACHJ8C_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542050.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            complement(14028..14681)
                     /locus_tag="ACHJ8C_003407"
     CDS             complement(14028..14681)
                     /locus_tag="ACHJ8C_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553207.1"
                     /translation="MIEQLQSDFLFWIFIYFLIVGLLIRNIHLTRKIKSIEKNKMFPA
                     EEYAVLDIEQHMSNKYIGRLVTRFMKRTNGAPLLIVFPEYDKGDNNEDKNSPPFQLVK
                     LTSNINNIKEIYSNDDLNCFLNNISVQKNLYTTHSGPSQAWIEHAYPLQQVTILLQGT
                     RHSEREDIISQLKIILSRLKNGENTGFEHDDDFGYSFQYDAAAVGKSSFFDEAAGRK"
     gene            complement(14693..15817)
                     /locus_tag="ACHJ8C_003408"
     CDS             complement(14693..15817)
                     /locus_tag="ACHJ8C_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO5553208.1"
                     /translation="MFQKIKIRKMTISRALDKYLKTVSIHKKGHLQEFYRVNVIKRHP
                     IAERYMDDITTVDIANYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIARVEWGT
                     CRMNPVELVRKPKISSGRDRRLTSGEERRLSRYFKEKNQALYVIFHLALETAMRQGEI
                     LSLRWEHVDLQHGVAHLPTTKNGAPRDVPLSRKARNYLQMLPTQLNGNIFSYTSSGFK
                     SAWRTALQELKIENLHFHDLRHEAISRFFELGTLNVIEVAAISGHRSLNMLKRYTHLR
                     AYQLVSKLDARRKQTSKIAPYFVPYPATVENRNGQVVVTLSDFDLETSAATKEQAIFH
                     ASVLLLRTLAQAAQRGERVPTPGELPTNIDERVMICPLTN"
BASE COUNT         4707 a         3112 c         3242 g         4815 t
ORIGIN      
        1 atcgcttttt gcaatattaa tatctttatt aatatcaatg ctgctttgta ttattttatt
       61 tactttgtct gacagaacgg catccagacg agcaggtgtc tcgtctatag cgttgataat
      121 taagtttgct gttttttcga tctgtttttc taaagcaata taatcttcaa gaagatcgtt
      181 tattttttga tctttaaact cttcagagat actacgagac attgtttttc tccaatttta
      241 tgaccgggta catcccggcc atattgtatc agacgagatt gaggttttta aaggcaacat
      301 caagatcgct gttgaatcca ttcatcaaca tttttactgc tcttaaattt gacaaatctt
      361 gccgctcttc ctttgaagac gctgaacgaa ttaatgaacg aaaatcacga ttgtcattat
      421 agacatcatc atactgtagg ccgcttttat ttaaaatatt gaaaatgttg ttatggtaaa
      481 caacaacagg attttcacct acaatttctt tacatacgtc gtcagctaag aaaactgaat
      541 attgtttgtt aataggggtg tccttttcag caagattgaa aattacttta aaacgatcct
      601 gttcaacacc catagccatt agtgttgtaa tggtttgaat tgagtctatt tgttgttttg
      661 cttctcgcac aacaggaacg atgaaataat caataaggtc atgacttccc tgatattcat
      721 tcatctgtac aaggaattgc tcaatatttg atgagcctac gtcaatgatg gtgcattcac
      781 tatcatcaat acgtttgata atttcgtcaa actcctttgc tgctaatttc tcgtcatttg
      841 taccatcagt gtttaatgtt tctactttta taatttctga cccttccaga cgaggtttca
      901 ataaagtttg accaatggtc gatttcccta cgtttccgct attatttaca attgctagtt
      961 ttaagtacat ttttcttacc ttttatttcg tatataattt ccaagagtat tgctaacttg
     1021 tacaaagtta gcacaattcc aagattttat tgtttctata gatacattgt tatctatagc
     1081 ttgttgagct atctttgcat tgttgaagca aactggcaaa tatgcattaa gtatatcatt
     1141 atcttgcttc gtttttctat ttttgtcctc aatggttatt gctttttgat acgttttctc
     1201 tttatttgaa agaagatgag aaacatcttt tttctcggcc ctttcttttc gtatgcgatg
     1261 caatatgttt tcaaacgtct ttaacgttag atcaaaacct tcatcattaa gagcattaac
     1321 aatatcaatg tttcttatgc cttgttgctg cgcttcttct atttcagcaa aacgctctct
     1381 tattcttcca gttttacttc gcttgacctt ctgagctaag agcgaaccaa gtagtttatt
     1441 ttctccattg ttcatttgtc agtctccagg acagtgaaat catgatgagc aattctatca
     1501 tgaacgtcaa gcaatttttg ctatgtatat gctatgtgtt tgctatgtat gtgctatgtc
     1561 attggttttt atggtgtgtt tttgctatgt attagctatg tttatggtgt gcatttgatg
     1621 tgtgaatgct atggaatcaa ataattttct caaacacagg cacgataccg attgtacaca
     1681 agaccgttcc ggtcatgtta caatcgtgtg ccaaaggggg atgccccttt gaaaccccga
     1741 acgacgcagc cgttcttatg cggttctacg aaccttgagg acatatgaag aaaaaaacca
     1801 acaaaaacgt tcatgtaaca ttcagactta ccgaagaaga atatgctccg ttcgataggg
     1861 ctataaaaga gcttaatatt agtaagtcag aatttttcag gctgcttact attggtaaaa
     1921 taaacacata tgcatctgat aaacgtaaca tcccagaata caaacgttgt ctttctcagt
     1981 tgagttgggc aggaaacaac ataaatcaaa tagcgcaccg attaaattca gatcatttaa
     2041 aaggtattat atcagaatcg ctttataaaa aggttttaaa tggactaatc ggtattcgtg
     2101 atcgccttca ggagatagct aaatgattgt cagatacggt ggtggtaatg atggtatcgt
     2161 tgattatctg ataaatggtc gcaaagcaga acgccagtac acgcgtgatg aacttgatca
     2221 tcgtgtcgtt cttgatggtg atttacagac tacagataag attattgatt ctattgaaaa
     2281 caaaagtcag gaacgttatt tgcacattac tctgtctttt catgaaagtc atgtgtcgaa
     2341 tgaagtgtta aaggctattg ttgatgatta taaaaagtta ttgatgaatg cttatcatcc
     2401 tgatgaatac tctttctatg cggaagctca tttgcctaaa attcgtcata tccaggataa
     2461 tagcaccgga gaacttgttg aaagaaaacc acatattcat attgtaattc ctaaagtaaa
     2521 tttgattact gaaaagttcc taaatccagt gggagatgtt accaaagggc atacaattga
     2581 acagcttgat gctatacagg aatttattaa taataaatat aatcttgata gccctaaaga
     2641 ctatccgcgt aaagatgcgg attacggaaa aattataagt agagttaagg gagatcttta
     2701 caaagagcat cactctgaat tgaaaggtga gttgctttcg cgtatagaga acgagaaaat
     2761 tgaaaattac tctgtattca aagatattgt tgcagagtat ggtgaacttc gtataagaaa
     2821 tgctggtaaa accaatgagt atctggctgt aaaattgccg ggagataaaa aatacacaaa
     2881 cttaaaaagt cctctttttc gtcagaatta cattgagaca aggacattaa cacttgagaa
     2941 accaacacac aaagagatag aaaagagact taatacctgg ttaaataaaa caagctatga
     3001 gattaagcat atttttaacc aggcggagaa aacaagggaa ctttacaaga cattaagccc
     3061 ttcacaacaa attgatttcc tgcaagagag gataaaagaa tatgactcaa gagaaaaact
     3121 taacgaaaga aattctcagc aaacgtcagg acgagcggga ggttacaagt cgtgtcctaa
     3181 aaagtttgcc agaatccgtc aatctgaagc aacagtcgga ttgtcacgta tgccccaacg
     3241 cggtatggtt tacggaatca atggattcac aagacccgac tctgtcagtg tattgtcaga
     3301 tatttcgcag cgtgatctgg cagagcaatt atcgcaaaga gaacatcctg gtcaggatgt
     3361 gcgacgggat tatgatagac aattcacaga gtcaggaata aaaagtcttg aacgctcttc
     3421 ttttttgtgt gaaacgatgt ttcagactct gaatgaggcg gcagagaaaa acgaaattgc
     3481 aacgatggca gaaattcgca gaaatattga tcctgtcagg tttctgtctt ctgccgccga
     3541 gcgttttaat attattccag cgcagcataa aatcagaaca gcaaaggacg gttccccccg
     3601 gttttctgtg gggaatcgta atatgaacgc atctgatttt ctgacaaagc atattaatct
     3661 tgcctggaaa gatgcgaaat cctttctgct tgaagtttat tcacagcagt tagaaaacac
     3721 gccatatacg cgctatccaa cctacagacg ccttacgtat catgaagccc gtgagcgtct
     3781 taactcactg aatttatcgg aaaaaacatt acgaaatact atcaggtttg agcgtggcaa
     3841 gctgtataat gatctgcgag agatgagacg cgagttgaag ttaataccac gtgaacagcg
     3901 tgatattgcc gtgggtgtga ttgtttacaa aaaactaacc acacttgagc gtctttctga
     3961 actcgacaca gaggggcgac acattattcg tcaatatcat gctgactggc ataaggataa
     4021 agatgaaatg aaagcccttg aacgtctcaa aagctatctc aacttcgatg aaatcaacgc
     4081 catttctgct gacgaacccg aactttcgct tcagaaagcg gtggattccc agcgccgctt
     4141 agaagaggcg aaaaaggtta actccaaact aaaagatctt gtgatggata agcaggattc
     4201 caggattgtt tatcgcgatc aggaatcgga aaagcctgtc tttaccgaca aagggaactt
     4261 tgtcgttgcg ggtaaaaatc cctcaaaaga agagatcgga ataatgcttg agtattccag
     4321 ggaaaagttt ggtggtgtac tcaaacttac cggctctgaa gatttcaaaa aaatgtgcgc
     4381 tgaagttgcc gcagagcagg acatgaagat tattttacgc ccggagcagt atcaacagat
     4441 gatgctggaa ttaaaagcag aacttcaggg taataagttt gagcaggtgg aaacacagga
     4501 aaatagccag gagtccgaat ccagaataga gaaaggtgat gcattgaagg aacaggccac
     4561 cgaacaggca caggccacag aacagactca ggccacagag caggcgcagg ccacagagca
     4621 gacgcaggcc acagagcagg cgcaggccac agagcaagca caggccacag agcaagcaca
     4681 ggccacatct tcatatgatc ctggtgttat taccagggca aataccctgg attcacagat
     4741 gcttagtaaa ggcacaaatg gtgaatttgg ctatttgaaa tctctggata gcgatgaaaa
     4801 tgagatttgg gaagtgctgg ggcatgtacc tggagacagc gatgatatct ttagtgttgc
     4861 cagttttgat aatgaaaacg atgcaaaaga attttgcaat attgttaatg aattaggaat
     4921 tgacagaaca caggctctga ttcaggagca gcttactact caacacgatc aggctactgc
     4981 gcaggtgcat aataaacagg aaatttattg tataaacttt agtcgcttcc atgatttgaa
     5041 tgaaggtatc gtctttcatt ctaaagatgc ggctattcag tgttatgagg aaagcaaatc
     5101 ttctgcaata gaaaaatatg aaagcaatta tttaaatggt gatggttttg atactgttgt
     5161 gcttatgtct aaaactgtct caaccgatga attatcctct tatccagagg gggcattagt
     5221 aacgtttgat aggccatttg aaataattgc caattcatat gaagaatacc gtaccccagt
     5281 gtatgcggtt tctttttcga aggatgagtt tagtgaagat attaagactt ttgagtcatt
     5341 gcacgacgca tctgaatata aaaataagat gcttcaggag cacgggctta accaggatga
     5401 tatcttaatc acacctgtaa caagagaaga aatcgccttt aaaggaatta aggacgctgt
     5461 taatgacgct aatatggcag tgatggaaca ggcgggggat tcgtccagag aatcaccaga
     5521 agagatactc gccagtattt cagctaacga gcatatgatc tccgggcttg aaaacttcct
     5581 ggttaaagac cgttctcagt tcagttcctg caatggggat atcgttgtgg aggcggaaat
     5641 taccaggggg gaaggtggtc tttatcgcct tgcggtcgct ggtaagcatg gcttagagcg
     5701 tggtgatgca gttgccagag tggatgttac ggagcagcaa tttgccgcga tcactggaaa
     5761 aacaccttct gaagttttaa cgggggacca aacatcggct cgtgttccgg taattacagg
     5821 tatccatttc agtacaaggg ctattgaaaa tgtgaataaa ctggaacaac agaaggatta
     5881 tgtctatttc tcaacacatg aaggtctgaa tgctgaaata aaagatttca gctctctcaa
     5941 ggatgctatt gagtggggcc gggttgaatg cgagctacat gatctcaata agcgcgatac
     6001 tgtcatttat cgcgtcgagt ctgaacatat ttctcagggg attgacgctg taatgaaaaa
     6061 tgctgaacgc gtggagcgtc atgaaattga gaaagcacag ggacgggatt gcacgccgga
     6121 agacggcaag attcttgagg caattgatcg ctttgaggac aagttcagag gggaaggact
     6181 gaagttcgaa agggagaagg ccgaatccga tttgcttaat cacggattca ctcgtgaaat
     6241 ggcagaagat gctcttggaa aacaatttgt ccaggccaga gaagatcatc aggaatcgca
     6301 gcagcagcgt gacgattcac aggacatgca ctaattaaga aaaaagcccg gttttcgggc
     6361 tttttaagaa aaaaagtcaa ccccaaacag aaataatagc tacaaaaatg gacagtaata
     6421 atgcaataaa agtaatgttg agccatatgt tggacgagtc ttttaagact aatatggctg
     6481 ttaatgccat tgtaatgaaa ctaatggtaa taatggctgc aaataaatat tgtacaatgt
     6541 tttttattgt acttggttgc tccccaattc tttctctgta atttgcatca acattagata
     6601 tccattgaag taatagagca ataaaacagc ctactccgca aaaggtgaat tgcacagacg
     6661 ttgggtttga atcaaacaac gagatcgcta agttatataa gtatatcgca cctgaaatca
     6721 ccccgacaac taaaatagag attagtataa tccgaagaac taatgtattg ctttccttta
     6781 aggaatatat taaagccgtt ttttcgtatt ctttcatgat ttaatccggt ctataagtat
     6841 aagaaccaac aaaactttct caacctcaac gttaacatca agacacctca cttcataaat
     6901 gatcgcctca gtatgtcaaa cagaaaaagg caaaaagcac tggcggacag acacggtagt
     6961 gtttcaaatg gattttttga caaaatgccg tagccagcga aaaaaagact aaacagaagg
     7021 gggatacaaa gaacaaacaa tttgaatatc ttcatagatc tttatccttc tttcatcatg
     7081 gctagttccg taatcctgtt ttctggacgg cacttttcag gcagatagaa tgatttattg
     7141 tcagggaata cccattcatc tttatcaacg ccgaataaac caccttgttg agcatccagt
     7201 gcctttaatt tgcatgtttt gggtattgat tcatgtagtt cctgcctttt ctctgtttcc
     7261 tgaagtattt cctttggtgg catgataagt ggcatgataa ctgcgggggg aatcatcaga
     7321 gcatgtaaca tacttacgaa taaaagccat cgacatactt gatcctcgat atcattggtg
     7381 atgctatatg ctatccctgc attgaaaaaa aagaatatca tatagacaca aatatattgt
     7441 ggctgaatga caattaactt tatcaggtag tagaagaaat tgtaggtctc gtagaggaaa
     7501 aacattcata ccacctataa cactattaag atagcctgcc agatatccat ttcagacagg
     7561 ctatatacag ggttcaattc attctatgaa aggtgtatag ggattgctga tgtttgtgtc
     7621 gatcaaaaat ctaatattaa tagatgaaaa ctaatataca tatttccaga cggatggagg
     7681 gactttatcg tagagtttat tcattgtgag ttccgcaagc ctgtgatcgg cggcggcatt
     7741 aaacgactca agctcagaag gggggaggca gttttggttt ttttcaataa tcttttcaag
     7801 tgtgtcgagt gttgagcaac gtcttaactg aaagagccag tcttgttttg ttttcattgt
     7861 acaattcctg aagaacatcc ttgccctata atagctacaa cgatgtttga tttctactgc
     7921 tgggtttatt gacgggagaa cgggcagaag aaaagccgct gccgcgcccc cgttcttatc
     7981 ggaagtaaat attataatta gtaattaaga caagaacgaa aaacaaaagc ggaaacacat
     8041 ttttaatggg ggggactgcg ttcgccagcc ccattgtact tacaaaactg aaagcagcca
     8101 gtaatagtga aaacataccg aaaaaacacg tctcaataac attgcccgtg ggaaaagata
     8161 ttccgataga tttagccagg gaagacacta ttccgaaaga gttagccacg gaagacacgg
     8221 tagttaaaac gacaaagaaa ttgttaacag ggaaaacaga gtcgtttctg gcaattgtgc
     8281 tatctttatc cttttttaaa ataataaatt tcatttatcc atccccctaa agcaacgtct
     8341 aataatctat gttatgcgca tcaatgagtg cacgtagatg ttctaactct ttctcttgca
     8401 tctggcatag atgatagaga ttatcacatt tcgtgcgata ttcagagcat tcagaaagaa
     8461 gactggctgt tctcaaatgt aacatcagga ttatcactaa aaaaactaat acactaacag
     8521 cgaaaagcaa caaaccattc accataaaat ctgtactctc cacgccgaga aacgaaacat
     8581 gagaattatc atcaaaataa acggatattt caaaggataa cgccctccag agaaaaggag
     8641 ggctgtagaa tcatttctcg atgagaaact catcaaggga acgacctcct gccagtgctt
     8701 cagcaatcgg gtttggcgct cgcccacgac cagacttggc tattaactga agcaattcct
     8761 gacctgaaaa gccatttctg ctttgctttc acttcttagc ccttgccgga aaacccagct
     8821 tccctgtttt cttatcctca aggacaagaa ccgcatcata cgttccaccg cttttctttt
     8881 taaatccctt aatcaaatcg gtttttcctg atttaataag tttttcggcc tgtgcctggg
     8941 tgattttctt gccagaaaac tcattccaga ttttaaattc acatcctgta caggaatagc
     9001 tttttggcct gataacaatc tgtttaccac aggaagggca aggagaggac aggcgttgtg
     9061 actgaccaga cggcgcgctg ggggaaactt ttatttcagc actgttcgca ttactgatca
     9121 tcggtatcag atcattgtac agttcgtcaa cgaactgttc gatggtcatt tctccgtttt
     9181 caatgagtgt ctgtttttcc gcccataatg ccgtcatatc tggattaaca gctatatccg
     9241 gcagagcatc aataagcgca tacccggtat ctgtcggaat aagttttcct ttttccaggg
     9301 tgatatagtt ccgttttttc agtgtttcca atatggaagc ccgcgtagct ggcgtaccaa
     9361 taccgccatg ttcgtctttt ttatcccgat ctttatcctt cagtaacttt ttaatcactg
     9421 gatcagtgac aaaatctgcg acacgaacaa gcgcggcaag taaagtggct tcggtgaata
     9481 atggcggtgg cgttgttttc ttctcattca caaccacttc ttttgtcgtc actgtttctc
     9541 ctgtgcgaat tttacagaga agatcaaaag cggaatcatc attttcgact tctgcttcat
     9601 cgtccccggc attttccaca ccaagaaacg cttcaaatcc gctgtctgta gttttcctgg
     9661 cacgggcata aaacgactca tcaccacact gaatggcgac ggatacttcc tgatatgcct
     9721 tttcaggcat gaactggaca agatagtgtt gtgcaattgc caggtaaacg ttgcgttcgt
     9781 cggtgctcag tgcgtttacg tccggtacgc taacggttgg gatgatggct gtatgcgctg
     9841 ttacttttgc gctgttaaat gccttgcttt tacgtgtcgt atcaatatcc attggctgat
     9901 caaacaccga cttcagggca tcgattattt gtggtgcttc actgaattgt tcgtctgaaa
     9961 gatatgaaca atctgaacgg ttgtaagtaa ttgctttgta tttttcgcgt agctgctgtg
    10021 tgatatccag cgttttttga gcagtcattt tgaatttttt gttcatgtac tgctgaagtc
    10081 gtaccaggtt aaagggcaac ggtgcagccg tttttttatc atctgttgct gccgcttcaa
    10141 ctgtggctgg ttttcctgca agtgatgttg ctgttccatt cgcccaggtt ttatcaagca
    10201 atttgcggtc tgtcagcggt gcgaactcac caggtttcca gttcgccctg ataacatcag
    10261 cgccgcgttg aaaatgtccg gtcatggtgt agtagaagct ggatttatga tttttattgg
    10321 cacgggtgcg atttacaatc aggccaagca cgggtgtctg gacccgtccg acagacaaaa
    10381 cacctttata gcctttagtt tttgccggga ttgtgtaagc acgcgtcatg gataaaccgt
    10441 atatcgcgtc ggctacagaa cgcgccagag ccttgagata gagtcctctg aagtcacggt
    10501 tatttttggg atttgccagt gcctttttca ctgccggaag ggtgttgtcg ttgatcagaa
    10561 cgcgcttaac gggttttgtg ttgcctgcat attccaggac ctcatcgaca agtagctgtc
    10621 cttcgtcatc tggatcgcca gcatgaataa tttcggtcac atcggcacgc ctgataagct
    10681 caataatcgt tttaacttgt tttgcggcgc tttctactgg ctggtatttt acgggataaa
    10741 gacgcaaagg gagtgtttca atcttccact ctttgtattc aggattatag ttttcggggg
    10801 gctgtgattc gataatatga ccaaaacagt tagtcacaat agtattatca ctttcaaacc
    10861 agccatcatg acgggtaaaa ttgccaccaa gcgccttaac aatatcattc gctactgcgg
    10921 gtttttctgc gataaaaagt ttcattttgt tatcctttac attgaattaa tcttttggca
    10981 caacggatag cgcgttcttc tgatactgcc tgttgccagt tatcttcact tacaggttgt
    11041 ctgaatctgt gctcacagcc attaaaggca aaaattccct cacggttaag tatattagtt
    11101 gcaatatccc agtctttaat tctcaaaaag gctgttacag caaaaccaga atacgaagaa
    11161 atttttgaaa tgatgcattc atcagctttt attaacgcgg cgaaaggaac tatcttttga
    11221 tgaccacacg aaatcagagg gagtgtgtga atatcataat aagctattga atgacaatcc
    11281 atgcgggtat catcaatagc tgccagccag aatcctgtct ggtcttttat ttttctgcta
    11341 atatctgaat ctgaaacacc aggatttaat aaatatccac cataacagac atggaagggg
    11401 tgagcagatt catcaatatc aggaagttct tttaccggat tttctttcca gtccttcaga
    11461 acaccgaatt caagagcatc cagcaaaaac tttcgttcaa atccatcaaa caatgcatat
    11521 tgcatgtaaa acctctcaaa gaagctctga tttgttgacc tcatggtgaa acagttcacc
    11581 attgcgtaag acaaacaggt aagaaaactt tctttcctta tctttcagcc agtttttata
    11641 ttcagaaagg ataaacgccc aatcatcggc gctgtaatga gattcgatga ctcattatcc
    11701 ttttagtgat gagttgatta tttttaaaat atgcctgcct ttaattagca tgtttctgga
    11761 aacagcctga ttacgaatac ctctcatctg gtcaaactga taggttaaat catgttcttt
    11821 tcgtgaaagt ccaagagtag ctctttcatt atccatcagg actgaaattg caatatattc
    11881 tccagatgca caccagtctg cataactggc aacgcaatgt tccatctctt gaccttcctg
    11941 acacagagca tcaaatgtaa ttaattcatt tacacagtta tcaattttct gccagtttat
    12001 cccggttcct ttccattttt gggttgcggg aataacgtta ttcttcactt gccgcgtcca
    12061 ttcatcagat agtctccaga aagaaggcca ctcctggttt ttatgaatta accgttcttc
    12121 tgctaacaac cagtcaattg catgacacaa ctgattcgtt tccatttccc agcgtgatgt
    12181 ttgcctggat tcagtattac gatcataaaa gccaatgttt ttaaataaat cacgatgata
    12241 ttcaagccat tttgagcata tacgataaat atcattttct ttttctgggt aagctcgaat
    12301 gctaatataa tcgataatcc agtaaattgc tcttacagga tagttcttga taagtggatg
    12361 gcgtaataat ctgactgtta atctgaatga tgcattatca tggttataaa ttaaagcata
    12421 tactaatgat aaactaacat gtcgtaaact tctgaactca ccataactaa gctcataggg
    12481 aaaacaaagt ctttcatata actcttttgt ctttcctgat agcataaact gacctttttt
    12541 atgttgttgt cttagaagac caaccatagg ccacaatccg caaaaatcta tatcatccag
    12601 aatatggatt cgctttgaaa aatccagata gtcctcaaat aatgcctgac gaattgaatt
    12661 gtaagctatc gaacaatgtg aagctctgga atattgtgca aatatctttt tatctaccat
    12721 tatccatatg gctttgctca atgcttttga agcattaagc tcattatcca cccctataat
    12781 ttttaatgct ctgtgataaa ttttgttttt tatctcttcg agtaaatcat gtctgctgat
    12841 atattgtacg ttatcggggt agttgaatga tgctgtttta ccaaggcaat gaataatgcc
    12901 atatcttcca taagaatacg agttatatac actgagtgga tatagtgtgg aaacataacg
    12961 actctcactg tgttcctctt catctccaac gcggaacaaa tagagtttgt cttcaattga
    13021 atatccagcc tcaatcaatg atttgatgat gtagctaatc gcatatttta accagtcgtc
    13081 catgtcgtat gcagattttt tagaccagaa taaaaaaatc ttaccaggaa atgaatttct
    13141 tctggtccat accccagtgt gcttatctct tagcttgtat ccccacaaag tgattttgtt
    13201 attctttttg ttttttctaa tacgaaaaac gccacaaatg ataatggaga tatacttttc
    13261 attctcctct gtttttacaa gacgaagccc ccctgtattt tgatagattt taaaaacatc
    13321 atgcaacgta agaatattat tgtcccttgt ctgttgatta attacataca tgccgccccc
    13381 tgttaaaaag aagcctttaa tattgcacaa agataaaaag acagaatcat ggcaaaccaa
    13441 aagaagaatg cattttcttg cccccttaat ttctgatacg ccattcccac ggtggaaccg
    13501 gatttgtatc agcccacaaa ccgcgttttt gtttctgcgc ttcccgttgc agagccggta
    13561 atgactcatc cgcgttgtaa cgttcatata cccatgcagc gccagactga accataaagc
    13621 ggctggcatc cgtgccgttc gtcgtgaaca catgaccgat aatgcgtccg tagcggtctt
    13681 tatgtgaata agagactgtc acctgtttac ctgcaaccag agttttaagc tgactagttg
    13741 accagcgtcc aaaaggttgt ttcttttcag gggcatcgat gtttatcaat cggactcgga
    13801 tcggaacttc atacacaaca atttttgcgg gtagtgtttt aatttctatg gtgtcgccat
    13861 cgagaacgcg aataactttc ccctgaagaa ttttctggtt aaatgtataa ggtattccac
    13921 ctgcatttgc agactgaaaa gcggtaagca gataaataaa tattgttaat aagattttca
    13981 taaaagatta acgttaattt gcctgtgcaa tggatagagc gttattctta ttttcttcct
    14041 gccgcttcat cgaaaaatga tgattttcct actgctgcgg catcatactg aaatgaatat
    14101 ccaaaatcat cgtcatgctc aaaacctgta ttttctccat tttttagtct ggaaagaata
    14161 atcttaagtt gactgattat atcttccctt tcactatgac gagtgccttg taagagaata
    14221 gttacttgtt gcaaaggata tgcatgttca atccatgcct ggctaggtcc agagtgcgtt
    14281 gtatataaat ttttttgtac cgaaatatta ttcagaaaac agtttaagtc atcattggaa
    14341 taaatttctt taatattgtt gatgttggat gttagtttta caagttggaa tggtggagaa
    14401 tttttgtctt cattgttgtc tcctttatcg tattctggaa acacaattaa caaaggtgca
    14461 ccattagttc tcttcataaa acgggttact agccgcccaa tatatttatt ggacatatgc
    14521 tgttcaatgt ccaggacagc atattcctcc gctgggaaca ttttgttttt ttcgatggat
    14581 ttaatttttc tggtaagatg aatgtttctg ataagcaaac ctactatcag gaagtaaata
    14641 aatatccaga ataaaaaatc actctgtagt tgttctatca taagtgaatc ctctaatttg
    14701 ttaatggaca aatcattacc cgttcatcaa tgtttgtagg aagttcgcca ggagtaggga
    14761 cgcgttctcc tcgctgggcg gcttgtgcca gtgttcgtag cagcaacacg ctggcatgaa
    14821 atatggcctg ttcttttgtg gctgcgcttg tttccaggtc gaaatcactc agagtcacaa
    14881 cgacttgccc gtttctgttc tcgaccgttg ccgggtaggg aacaaaatag ggagcgattt
    14941 tgctggtttg cttgcgtcgg gcatcgagtt tactgacaag ttgataagcc cgaaggtgtg
    15001 tgtatcgttt caacatgttc agggagcgat ggcctgaaat agcggcaacc tcaatgacgt
    15061 tcaatgtacc aagttcgaaa aatcggctga tagcctcatg acggaggtca tgaaaatgca
    15121 agttttctat cttcagttcc tgaagtgctg ttcgccaggc acttttgaat cccgaagagg
    15181 tataagaaaa aatattaccg ttaagctgtg tcggcagcat ttgcaggtaa ttacgggctt
    15241 tcctggatag cggaacatcg cgtggagcac cgtttttggt tgttggaaga tgagctaccc
    15301 catgttgcag atcgacgtgt tcccaacgca atgacaagat ttcaccttgt cgcattgctg
    15361 tttccagcgc cagatgaaaa atgacgtaaa gagcctgatt tttttctttg aaataccgcg
    15421 aaagtcggcg ttcttctcct gaagtcaggc gacgatcgcg cccgctactg attttgggtt
    15481 ttctgaccag ttcgacagga ttcatgcggc atgttcccca ttccacacgg gcgatattga
    15541 ataatgatga caacagggca agttcaagac ggacggtgtt tccggttatt tgacgccccg
    15601 ttcgtgggtt aatttgcgcc aaccgttgat cccgataatt ggcaatatca acagtggtaa
    15661 tgtcatccat atagcgttca gcaatagggt gacgtttaat cacatttacg cgataaaact
    15721 cctgtagatg gccttttttg tggatggata ctgtcttcag gtatttgtcc agcgcccggc
    15781 tgattgtcat tttgcgaatt ttgattttct gaaacatatc ccctccagac aaaaaagggg
    15841 ggaaggtaga gattcttgct tcgaaatgca atatca
//
LOCUS       DAZFJL010000108        15840 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFJL010000108 DAZFJL010000000
VERSION     DAZFJL010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.108"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(17..742)
                     /gene="phoU"
                     /locus_tag="ACHJ8C_003409"
     CDS             complement(17..742)
                     /gene="phoU"
                     /locus_tag="ACHJ8C_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO5553209.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVERQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAEKDSDK"
     gene            complement(757..1530)
                     /gene="pstB"
                     /locus_tag="ACHJ8C_003410"
     CDS             complement(757..1530)
                     /gene="pstB"
                     /locus_tag="ACHJ8C_003410"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO5553210.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(1621..2511)
                     /gene="pstA"
                     /locus_tag="ACHJ8C_003411"
     CDS             complement(1621..2511)
                     /gene="pstA"
                     /locus_tag="ACHJ8C_003411"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO5553211.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMIAAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2511..3470)
                     /gene="pstC"
                     /locus_tag="ACHJ8C_003412"
     CDS             complement(2511..3470)
                     /gene="pstC"
                     /locus_tag="ACHJ8C_003412"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO5553212.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(3557..4597)
                     /gene="pstS"
                     /locus_tag="ACHJ8C_003413"
     CDS             complement(3557..4597)
                     /gene="pstS"
                     /locus_tag="ACHJ8C_003413"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO5553213.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(4911..6740)
                     /gene="glmS"
                     /locus_tag="ACHJ8C_003414"
     CDS             complement(4911..6740)
                     /gene="glmS"
                     /locus_tag="ACHJ8C_003414"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO5553214.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(6902..8272)
                     /gene="glmU"
                     /locus_tag="ACHJ8C_003415"
     CDS             complement(6902..8272)
                     /gene="glmU"
                     /locus_tag="ACHJ8C_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO5553215.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHIIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGAGCVIKNSVIGDDCEINPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPIKKK"
     gene            complement(8626..9045)
                     /locus_tag="ACHJ8C_003416"
     CDS             complement(8626..9045)
                     /locus_tag="ACHJ8C_003416"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO5553216.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(9066..10448)
                     /gene="atpD"
                     /locus_tag="ACHJ8C_003417"
     CDS             complement(9066..10448)
                     /gene="atpD"
                     /locus_tag="ACHJ8C_003417"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO5553217.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(10475..11338)
                     /gene="atpG"
                     /locus_tag="ACHJ8C_003418"
     CDS             complement(10475..11338)
                     /gene="atpG"
                     /locus_tag="ACHJ8C_003418"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO5553218.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(11389..12930)
                     /gene="atpA"
                     /locus_tag="ACHJ8C_003419"
     CDS             complement(11389..12930)
                     /gene="atpA"
                     /locus_tag="ACHJ8C_003419"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO5553219.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKSILDSFK
                     ATQSW"
     gene            complement(12943..13476)
                     /gene="atpH"
                     /locus_tag="ACHJ8C_003420"
     CDS             complement(12943..13476)
                     /gene="atpH"
                     /locus_tag="ACHJ8C_003420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO5553220.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(13491..13961)
                     /gene="atpF"
                     /locus_tag="ACHJ8C_003421"
     CDS             complement(13491..13961)
                     /gene="atpF"
                     /locus_tag="ACHJ8C_003421"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO5553221.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(14023..14262)
                     /gene="atpE"
                     /locus_tag="ACHJ8C_003422"
     CDS             complement(14023..14262)
                     /gene="atpE"
                     /locus_tag="ACHJ8C_003422"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO5553222.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(14309..15124)
                     /gene="atpB"
                     /locus_tag="ACHJ8C_003423"
     CDS             complement(14309..15124)
                     /gene="atpB"
                     /locus_tag="ACHJ8C_003423"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO5553223.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(15133..15513)
                     /gene="atpI"
                     /locus_tag="ACHJ8C_003424"
     CDS             complement(15133..15513)
                     /gene="atpI"
                     /locus_tag="ACHJ8C_003424"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO5553224.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         3809 a         4299 c         3952 g         3780 t
ORIGIN      
        1 ggatttactt ggtgaattat ttatcgctat ctttttctgc cagcagttta tcgagctcgt
       61 caccaccgac gtgacggaaa tcctgcccct tcacgtagta gaagataaac tcacaaatat
      121 tctggcagcg gtcgccaata cgttcgatag aacgcgcaca gaataacgcg gtaagtacgc
      181 tcggaatggt acgcgaatct tccatcatgt aggtcatcag ttgacgaaca ataccttcgt
      241 actcctgatc gactttttta tcttcacgat aaatacgtac cgcttcgtca atgtccatcc
      301 gcgcgaacgc gtccagcacg tcgtgcagca tctggatggt atgacggccc agcgactcca
      361 gacttaccag caatggctga tgctgctggg agaatttctc cagtgcagta cggcagattt
      421 tgtccgccac gtcgccaata cgctccagct cggcaatggt tttactgatc accataacca
      481 gacgcaggtc gctcgccgtc ggctgacgtt tggcgataat gcgcacgcag gcttcatcga
      541 tcgccacttc catcatgttg acgttcttgt cgccttcgat gacgcgcttc gccagatcgc
      601 tgtcctggtt atgcatcgcg gtgattgcat cagaaagctg ccgctccacc atgccgccca
      661 tggtcatcac ctgcgtgcgg atgctttcca gttcggcgtt gaactggccg gaaatatgtt
      721 tattaagatt gagactgtcc ataacgcact cctgaatcaa ccgtaacgac cggtgatgta
      781 gtcttctgtt tgtttcttcg ctggcttggt gaacagatcg tccgtgttgc tgaactcaat
      841 caattcgccc aggtacataa acgccgtgtg gtcggaacaa cgcgcagcct gctgcatgtt
      901 gtgggtgacg atcaccacgg tgtaatcctg cttcagttcg gtgatcagct cttcaatacg
      961 cccggtagag atagggtcga gtgcagaaca cggttcgtcg agcagcagca cttccgggcg
     1021 aatggcgata ccacgcgcaa tacacaaacg ctgttgctga ccaccagaga gagagtaacc
     1081 gctctggtgc aatttatctt tggtttcgtt ccacaatgcg gctttggtca atgcccactg
     1141 cacgcgctcg tccatgtcag cacgggagag cttctcaaac agacgcacgc caaaagcgat
     1201 gttgtcgtag atggacattg ggaacggcgt cggtttctgg aacaccatgc ccactttcgc
     1261 acgcagcagg gcgatatcct gagagttggt caggatgttg tcgccatcga gcagaatttc
     1321 accttccgca cgctgttccg ggtacagttc aaacattttg ttgaaggtac gcagcagcgt
     1381 tgatttaccg cagccggacg gcccgataaa cgccgtaacc tggtttttgg cgatatccag
     1441 gttgatgttt ttcagggcat ggaatttgcc gtagtagaag ttcaaattac gaacctgaat
     1501 tttactcggg gcagtttcaa ccatactcat tgcaatcttt tcctcatcgt gcctgatgca
     1561 cttcgcgaat caggcttacc gtaggccgga taaggcgttt acgccgcatc cagcaaagtg
     1621 ttaaccgtgt ttattcttcg caaaaacaac gcgcgccaga atgttcagca gcagtacgca
     1681 cagggtaatg atcaacaccc cggcccaggc cagttgctgc cattccgcaa acgggctcat
     1741 cgcaaactta aagatcgtca ccggcaggtt ggcgatcggc tgcatcatgt ccgtgctcca
     1801 gaactggttg gagagcgcag taaacagcag cggtgcggtt tcaccagcaa tacgggcaat
     1861 cgccagcagg ataccggtca tgatcccgga caccgacgct ttcagcgtga tcgcggcgat
     1921 catcttccac ttcggcgtac ccagcgcata agccgcttca cgcaggctgt ccggcaccag
     1981 tttcagcatg ttctcggtgg tgcggataac aatcggcacc tgcaacagcg ccagcgcaat
     2041 cacgcccgcc cagccggaga agtgctccat ctgcgccacc acgatggtgt aaacaaaaag
     2101 accaaccaca atcgacggcg cggagagcag aatgtcgtta atgaagcgaa tcacttctgc
     2161 cagccaggat ttacgaccat attcagccag gtaaatcccc gccataatgc ccagcggcgt
     2221 accgaatacg gtagcccaca aaattaacag gccgctaccc gccagggcgt tcgccagacc
     2281 accgccttcc gtattgggtg gtggcgtcat ttcggtgaac agcgccagcg acataccgtc
     2341 gataccgcga gtgatggtgg acattaaaat ccagatcagc cagaacagac cgaaggccat
     2401 cgtcgccatt gagagcgtca gcgcaatacg gtttttgagg cggcgacgcg cctgcatttt
     2461 gcggcgggat tcagccagtg ccgcagtggt ttgcatttca accatagcca ttagcgtgcc
     2521 ccctcattct tagccaggcg cataatcata aacttcgatg cggcgaggac gatgaaggta
     2581 atcacaaaca ggatcaggcc cagttccatc agtgcagcga cgtgcagacc cgactctgct
     2641 tccgcaaatt cgttggccag cgcagaggtg atactgttgc ccggcatgta tagcgaggcg
     2701 ctgtcgagct ggtaggtgtt accgatgata aaggtcaccg ccatggtttc accgagcgcg
     2761 cgccccagcc ccaacataat gccgccgata acaccatttt tggtgaacgg aagaacgata
     2821 cgccagataa cttcccaggt ggtgcagcca ataccatagg ccgactcttt catcatcacc
     2881 ggggtttgtt cgaatacatc gcgcattacc gccgcaatgt acggaatgat catgatggca
     2941 aggatcacgc ctgccgcgag gataccgata ccaaaggccg ggccagagaa cagcgcgcca
     3001 acaatcggaa tattcgacat gatattgccg accggctcct ggaaataaac ggcgaacagc
     3061 ggcgcaaaga taaacaggcc ccacatgccg taaacgatac ttggaatggc agccagcagc
     3121 tcaatggcga tacccagcgg gcgtttcagc cagccaggcg caagctcggt caggaacaga
     3181 gcaataccaa aactcaccgg aacggcaatc agcagtgcga taaacgaggt taccagcgta
     3241 ccgtagatcg gcaccagcgc cccgtagata tcgttcggcg catcccactc tttggtccac
     3301 aggaaagcca gaccaaattt ctgaatgctc ggccaggatg agatgatcag agagacaata
     3361 atgccaccca acatcaatag cacaatcagc gccgccagtt ttaccagcac gctgaaaatt
     3421 atgtcgccct ttttacccgg tgggttaaaa gcaggcttgg ttgcagccat aagttactct
     3481 tcagttaaac gcgtttacaa agtcattttg taatgccgga tgcggcgtaa aacaccgtac
     3541 ccggcctgga gttttattag tacagcggct tgccgctact gtctttaata ttggtcttcc
     3601 acgcagcgcg aacctgttca actacgctat ccggcaggct ggcataatcc aggtcgttcg
     3661 cctgtttagc cccggttttg tatgcccagt cgaagaactt caacacttcg gtgccttgtt
     3721 ctggtttctt ctgatcttta tggatcagaa tgaacgtggt agaggtaata ggccatgcat
     3781 cttcgccttt ctggttggtc agatcctgag cgaatgtttt gctccagtct gcgccttttg
     3841 ctgcattagc gaagttttct tcggtcggac tcaccggttt gccatcagcg gagatcagtt
     3901 tggtgtacgc caggttgttc tgcttcgcgt aagcatattc aacgtaacca attgcacccg
     3961 gcagacgctg aacgaacgcg gcgataccgt cgttaccttt accgcccaga ccgatcggcc
     4021 attttacggt agagccagta ccaacgttgt ttttccactc ttcgttcact ttcgccaggt
     4081 agctggtgaa gacgaaggaa gtcccggagc catctgcgcg gcgtactaca gcaatgttct
     4141 gtgaaggcag tttcagaccc ggattcagtt tggcgatggc ttcatcatcc cacttcttga
     4201 ttttgcccag gtagatgtcg ccgagggttt taccatccag caccagttcg ccagacttca
     4261 gacctggaat attaaccgcc agcaccacgc cgccaatcac ggtcgggaac tggaacaggc
     4321 cttcctgagc cagtttttcg tcagataacg gcgcatcaga agcaccgaaa tcaacggtat
     4381 tagcgataat ctgttttacg ccaccggaag aaccgatacc ctggtagtta actttattac
     4441 cggtttcttt ctggtaagtg tcagcccatt tggcatacac cggcgcaggg aaggttgcac
     4501 ctgcacctgt caggcttgct tctgcaaaca cagagaaagc actcatcgat aaggtcgcgg
     4561 cgacaacagt tgcgacggtg gtacgcataa ctttcataat gtctcctggg aggattcata
     4621 aagcattgtt tgttggctac gagaagcaaa ataggacaaa caggtgacag ttatatgtaa
     4681 ggtttatgac agttttatga cagagagaga aagtctttag tctgatttaa ataagcgttg
     4741 atattcagtt aattacaaat attaataacg aagagatgac agaaaaattt tcattctgtg
     4801 tcagagaaaa agtagccgaa gatgacggtt tgtcacatgg agttggcagg atatttgatt
     4861 aaaaacataa caggaagaaa aatgccccgc ttacgcaggg catccattta ttactcaacc
     4921 gttaccgatt ttgccaggtt acgcggctgg tcaacgtcgg tgcctttgat cagcgcaacg
     4981 tggtaagcca gtagttgcag cggaacggtg tagaagatcg gtgcaatcac ctcttccaca
     5041 tgcggcatct caatgatgtg catgttgtca ctgctcacaa aacccgcatc ctgatcggca
     5101 aagacataca actgaccgcc acgcgcgcga acttcttcaa tgttggattt cagtttttcc
     5161 agcaattcgt tgttcggtgc aacgacgata accggcatat cggcatcaat caacgccagc
     5221 ggaccgtgtt tcagttcgcc agcagcgtag gcttcagcgt gaatgtaaga gatctctttc
     5281 agcttcaatg caccttccag cgcgattggg tactgatcgc cacggcccag gaacagcgcg
     5341 tgatgtttgt cagagaaatc ttctgccagc gcttcaatgc gtttgtcctg agacagcatc
     5401 tgctcaatac ggctcggcag cgcctgcaga ccatgcacga tgtcatgttc aatggaggca
     5461 tccagacctt tcaggcgaga cagtttcgcc accagcatca acagcacagt taactgggtg
     5521 gtgaatgctt tggtggaggc cacgccaatt tccgtaccgg cgttggtcat cagcgccaga
     5581 tcggattcgc gcaccagaga agaacccgga acgttacaga ttgccagcga accaaggtaa
     5641 cccagctctt tcgacagacg caggcccgcc agggtatccg cggtttcgcc agactgtgac
     5701 aaggtgatca tcaggctgtt acgacgcacg gcagatttgc gatagcggaa ttcagaggcg
     5761 atttcgacgt cgcacggaat acctgccagc gattcaaacc agtagcggga aaccataccg
     5821 gagttataag aagtaccaca ggcgaggatc tgaatatgct caaccttcga cagcagttca
     5881 tcggcgttcg gtcccagctc gcttaaatca acctgaccgt ggctgatgcg cccggtaagg
     5941 gtgtttttga tcgcgttcgg ctgttcgtag atctctttct gcatgtagtg gcggtaaata
     6001 cctttatcgc ccgcgtcata ttgcagattg gattcgatat cctgacgttt tacttccgcg
     6061 ccagttttat cgaagatgtt taccgaacgg cgagtgattt ccgcaatatc gccctcttca
     6121 aggaagataa agcgacgagt cactggcaac agcgccagct ggtcagaagc gataaagttt
     6181 tcgcccatac ccaggccaat taccagcgga ctaccagaac gtgccgccag cagggtatcc
     6241 ggatgacggg agtccatgat cactgtacca tacgcaccac gtagttgcgg gatagcacgc
     6301 agaactgcct cacgcagagt accgccttgt ttcagctccc agttcactaa atgtgcaatc
     6361 acttcggtgt cggtttcaga aacgaaggta tagccacgcg cttttaactc ttcacgcagc
     6421 ggttcatggt tttcgatgat gccgttatgc accaccacaa tgtgttcaga aacatgcgga
     6481 tgcgcgttag cttctgaagg ttcaccatgg gtcgcccagc gagtgtgagc aataccggtg
     6541 ccgccatgca gaggatgttc ttccgctgcc tgagccagca tctgtacttt accgaggcga
     6601 cgcaggcggg tcatatgacc ttcagcatcc accacggcca ggccggcaga gtcatatccg
     6661 cgatattcca gacgacgtaa accttcaaga aggatttcag ctacatcacg ttgcgcgatc
     6721 gcgccaacaa ttccacacat agtttttgat tccgatttat atcgttgtcg gtcaacctgt
     6781 atgcccgttt tttcgggcgc cccgagcctt gtagagagtg gggttatttt tatagttact
     6841 gcttgtgggc gggagattat gttatctcct catcccatat gaccggatta cccggccaga
     6901 attacttttt ctttatcgga cgacgccagc cttctttttg agtctgcggc acacggctga
     6961 tagccagggc attttcgccg acattacgcg tcacggttgt acccgcagca atggtcgcgc
     7021 ctttgcctac tgttaccggg gccaccagtt gagtgtcgga accgacaaac acatcgtcgc
     7081 cgataatagt cttaaattta ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga
     7141 tgttaacgtt atcgccaatt tccgcatcgc ccaggtaagt cagatgacca gctttcgagc
     7201 ctttacccag acgcgctttt ttcatctcaa cgaagttacc gacgtgtgca ccttccagca
     7261 actcagcacc aggacgcaga cgggcaaacg ggccaatagt acaggctgct gccagattcg
     7321 catcttccac gacggtatac ggattgattt cgcaatcatc gccaatcacg ctgtttttaa
     7381 tcacgcaacc ggcgccgatt ttcacgcgat gaccgagagt cacgttgccc tcgatgataa
     7441 cgttagtatc aatttcaaca tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg
     7501 ccggatcgcg cagcataacg cctgctaaca gcagtttttc agcctgttcg gactggtaaa
     7561 cgcgctccag tcgggagagt tgcaggcggt tattcacgcc ttctacttcg cttaaacgtt
     7621 gcggatgaac ggcgacgatt tcacgtcctt cctgatacgc cagcgcaatg atgtcggtga
     7681 tgtagtattc gccctgagca ttattgttgg tcagcttcgc cagccagcgt ttcatatctg
     7741 cgccgttggc aatcagaatg ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg
     7801 catctttgtg ctcaacaatg ccggtaactt tgccgttttc acgggtgata cgtccataac
     7861 cggtcggatc atccagtttt accgtcagca gaccaatgcc accctgcggt ttagcatcac
     7921 gcagacgctg gagtgtttcg acagagatca gcggcacgtc gccgtagagc attaaaatgt
     7981 cttcatcatc ggcaaagaaa ggtgcggcct gctgcatcgc atgacccgta cccagctgct
     8041 ctgcctgaag cacccagttc aggttgtcgt ctttcagcgc ctgcttaagc agatcgccgc
     8101 cgtgaccgta cacaaggtga acgtgcgctg cgcctaattc attcgcagca tcaatgatat
     8161 gctgaaccat cgctttcccg gcaagggtat gcagcacttt cggaagatcg gaatacatgc
     8221 gcgtgccttt gcctgcggca aggatcacta cgctcatagc attattcaac atacgcgtcc
     8281 tgactgtaat ttgagaacga atttaaaccg cttcaccttg aaaaaactac atttttttca
     8341 tcgtgaaatg gacagaggat aaattgttca ataacggatt atccccgctg acgaaagcgc
     8401 cattttcgac catcgtcacg ccttttgtct gcagaaaatt aaggcagtac cttcatttaa
     8461 gcgtcaggtg gatgtttttg ctcttatttc gatcaatgaa taaacgggaa aacaggggtg
     8521 ttttatatca tcaggatcta tgtgaacgct tttcaggacg ggtcaatacg cgtaaaaaaa
     8581 agccagcctg ttgccagact ggcttttgtg cttttcaagc cggtgttaca tcgctttttt
     8641 ggtcaactcg ataacgcgca gctgcgcgat cgctttggcc agttccgcag acgcctgagc
     8701 gtaatctacg tcgccgtgag agctgctaat gtgctcttca gccttacgtt tcgcttccat
     8761 ggctcgcgct tcgtcgagat cctggccgcg aattgcggtg tcggccagaa cggtcacgtt
     8821 gccaggctgc acttcaagaa tgccgccaga cagatagata aactcttcgt gaccgtgctg
     8881 tttcacgatg cgaatcatac caggcttaat ggcggtgagc agcggtgcgt ggccagggta
     8941 gatccccagt tcaccttcgc tacccgttac ctggattttc tcgaccagac cagagaacat
     9001 ttgttgctct gcgctgacga cgtccaggtg gtaagtcatt gccatatcac cctccgatta
     9061 aggcgttaaa gttttttggc tttttccaca gcttcttcga tggaaccgac catgtagaac
     9121 gcctgctccg gcaggtgatc gtattcgcct tccatgatgc ctttaaagcc acggatggtg
     9181 tctttcaggg agacgtattt acccggagaa ccggtgaata cttctgccac gaagaacggc
     9241 tgggacagga agcgctggat cttacgagca cgcgctacca ccagtttgtc ttcttctgac
     9301 agttcatcca tacccaggat ggcgataatg tctttcagtt cctgataacg ttgcaggatg
     9361 gactgaacgc cacgcgcggt gtcgtagtgt tcctgaccaa ccaccagcgg gtccagctga
     9421 cggctggtgg agtccagcgg gtcaacggcc gggtagatac ccagagacgc gatctgacgg
     9481 cttagtacca cggttgcgtc aaggtgcgca aaggtggttg ccggagacgg gtcagtcagg
     9541 tcatccgcag gtacgtatac cgcctgtacg gaggtgatag aaccggtttt ggtggaggtg
     9601 atacgttcct gcagaacgcc catctcttcc gccagggtcg gctgataacc taccgctgaa
     9661 ggcatacggc ccagcagtgc ggatacttcc gtaccggcca gggtgtaacg atagatgttg
     9721 tcaacgaaca gcagaacgtc acgaccttcg tcacggaatt tctcagccat ggtcagaccg
     9781 gtcagagcaa cgcgcagacg gtttcccggc ggctcgttca tctggccata caccagggat
     9841 actttgtcga taacgttgga gtcggtcatt tcgtggtaga agtcgttacc ctcacgagta
     9901 cgttcaccta cgcccgcaaa cacagagtaa ccggagtgct cgatcgcgat gttacgaata
     9961 agctccatca tgtttacggt tttacctaca ccagcaccac cgaacagacc aactttaccg
    10021 cccttagcga acggacacat caggtcaata actttgatac cggtttccag cagttcctga
    10081 gagtttgaca gctcttcgta ggaaggtgct gcgcggtgaa tcgcccaacg ctcttcttca
    10141 ccgatctcgc ctttcatgtc gaccggttca cccagtacgt tcatgatacg acccagagtc
    10201 gctttaccta ccgggacttc gatcgggtgt tcgaggtctt ttacatccag accgcgacgc
    10261 agaccgtcgg aggaacccat tgcgatggta cgcacgatac cgccgccgag ctgctgctga
    10321 acttccagca ccagacgctc attaccattt tgcacctcaa gagcatcgta cacgcgcggt
    10381 acggcatcct gagggaattc gacgtcaact acggcgccga ttacctggac aatctttcca
    10441 gtagccatct taaatcctct acgaaataac ctgtttaaac cgcggcggcc cccgagacga
    10501 tctcggtgag ttcctgagta atgctggcct gacgagcttt gttgtatacc aactgcagct
    10561 ctttaatcag gctgccgcca ttgtcggtcg cggctttcat cgccaccata cgggcggcct
    10621 gctcgctggc caggttttca accacgccct gataaacctg agattcgaca taacgacgca
    10681 gcagggtatc cagcaacgcc ttcggatcgg gttcgtacag gtaatcccag gatttatgtt
    10741 tcagatcatc atcatctgat gccggtaacg gcagcagctg gctgatggtc ggaacctgag
    10801 acatggtgtt aataaatttg ttgctgacaa tgtaaagctt gtccagacgg ccttcgtcgt
    10861 aggcctgcaa catcactttt accggaccga tcagttcgga cagggaaggg ttatccccca
    10921 tgccggtgac ctgggcaaca acattgccgc ccacggagtt gaagaacgac acgcctttcg
    10981 agccgatcat tgcgaggtcg cattgaacgc ctttgtcggt ccaggtcttc atttccgcca
    11041 gcagtttttt gaacaggtta atgttcaaac caccgcacaa accacggtcg gtcgacacca
    11101 ccaggtagcc cacgcgttta acgtcgcggt cttccaggta agggtgctta tattccagat
    11161 taccgtgtgc aaggtgacca atcactttgc gcatggtttc tgcataagga cggctggccg
    11221 ccatgcgatc ctgcgattta cgcattttgg aagcggcgac catctccatc gctttagtga
    11281 tcttttgcgt gttctggacg cttgcgatct tactacgtat ctcttttgcg ccggccatga
    11341 gcttctcctc aatgccttgc ggcctgccct aaggcaagcc gccagacgtt accaggattg
    11401 ggttgctttg aaggaatcga ggatgctttt cagcttgcct tcgatttcgt cgttgtagcc
    11461 accggtctgg ttgatctctt gcatcaacgg agcgtgatca cggtcgacgt aagccagcag
    11521 agcggcttcg aagctgccga ttttcgacag ttcaacatcc gccaggtaac cacgttctgc
    11581 tgcgaacaga accagagact gttgcgcaac agacatcggc gcatactgtt tctgcttcag
    11641 cagttcggtc actttctgac cgtggtcaag ctgcttacgt gttgcatcgt caaggtcgga
    11701 tgcaaactga gagaacgctg ccagttcacg atactgtgcc agagcggtac ggataccacc
    11761 ggacagtttt ttcatgatct tggtctgtgc tgcaccacca acacgggata cggaaatacc
    11821 cgggttaacc gcaggacgaa taccggcgtt gaacaggttg gtttccagga agatctgacc
    11881 atcggtaatg gagattacgt tggtcggaac gaacgcagaa acgtcacccg cctgagtttc
    11941 gataatcggc agcgcagtca gagaaccggt tttccctttc acttcacctt tggtgaaagc
    12001 ttcaacgtat tcggcgttaa cacgcgcagc acgctccagc agacgagagt ggaggtagaa
    12061 tacgtctccc gggaatgctt cacgtcctgg cggacgacgg agcagcaggg aaatctgacg
    12121 gtaagcaaca gcctgtttag acaggtcatc gtaaatgatc agcgcatctt caccgcggtc
    12181 acggaagtat tcacccattg cgcaaccagc gtacggtgcc aggtattgca gtgctgcgga
    12241 ttcagacgcg gttgctacca caacgatggt gttagccagt gcgccgtgct cttccagttt
    12301 acgtaccacg ttagaaatgg tggacgcttt ctggccgata gcgacataga tacatttgat
    12361 accggaatcg cgctggttga tgatggcatc gatagccagt gcggttttac cggtctgacg
    12421 gtcaccgatg atcaattcac gctgaccacg accgattggg atcatggagt caacagcttt
    12481 ataaccggtc tgtaccggct gatctacgga ctgacgttcg ataacgcccg gagcgattgc
    12541 ttctacagca gagaagccgt cgtgatccag cggaccttta ccgtcgattg gtgcacccag
    12601 agtgttaacc acacggccca gcaggccacg gccaaccgga acttccagga tacggccagt
    12661 acacttaact ttcatgcctt cggcaaggtc agcgtacgga cccataacaa ccgcacctac
    12721 agagtcgcgc tcgaggttca gtgcgatagc gtaacggtta cccggcaggg agatcatttc
    12781 accctgcata caatcggcca ggccgtgaat gcggataaca ccgtcactta cagaaacaat
    12841 agtaccttcg ttgtgagctt cactcacaac attgaactga gcaatgcgct gcttgatcag
    12901 ttcgctgatt tcggtggaat tcagttgcat gctccagtcc ccttaagact gcaagacgtc
    12961 tgcaaggcgc tcaagacgac cgcgtacgct gccatcaatg accatatcac ccgctcggat
    13021 gataacgcct gccattacag acttatcgat tttgcaattc agcttaactt tgcgtgacag
    13081 acgtttttcc atcgcagcag aaattttcgc gagctgttgt tcactcagtg cggcagcgga
    13141 aatgacgtct acctcagcgg tagcctcact cacggcacgc aggtgaataa actgctccag
    13201 aacatccggg agcgcgttaa gacgaccatt ttcagccatt acccgaatca ggttctgacc
    13261 gttttcgtcc agttgctcac cacaaactgc gataaacgac tcggcgagcg tttctggcgc
    13321 aagcgcgcca gagagaagct ctgccatttg ttcgtttttg gttacctcgg cggcaaacgc
    13381 cagcatgtcc tgccagcgtt ctacactttg gtgttcgacg gcaaagtcaa aagctgcttt
    13441 ggcgtagggg cgagctaccg taataaattc agacatcagc ccctccctcc ttacagttca
    13501 gcgacaagtt tatccacgat gtcgctgtta gcagcttcat ccacggaacg ttcgatgatc
    13561 ttctcggcgc cagcaacagc caggatagca acttgcttac gcagctcttc acgggcacgt
    13621 ttacgctcgg cttcaatttc cgcctgcgcc tgggccacga ttttagtacg ttcctgttct
    13681 gcctcagctt tcgcttcgtc cagaatctgc gagcggcgtt tgttcgcctg ctcgatgatt
    13741 acctgggctt ccgctttcgc ttttttcagc tggtcggtcg cgctggcctt tgcaaggtca
    13801 aggtccttat gtgctcgttc tgcggaagca aggccgtcag caatttcttt ttgacgtttt
    13861 tcgatggctg ccattaatgg cggccatacg tacttcatgc agaacagaac gaacaggaca
    13921 aacgcgatgg cctggccgag gattgttgcg ttaagattca cagcacaatg cctctattta
    13981 gttaacgttc tgatattgct ctttaaataa aagcaacgct tactacgcga cagcgaacat
    14041 cacgtacaga cccagaccta cagcgatcat cgggatagca tccaccagac ccataacgat
    14101 aaagaactga gtacgcagca gaggaatcag atcaggttga cgcgctgcgc cttccaggaa
    14161 tttacccccg aggatgccga taccgatcgc agcaccgatt gccgccagac ccatcatcac
    14221 agcggcagcc atgtacagca gatccatatt caggttttcc atgacagtct ccagtttgtt
    14281 tcagttaaaa cgtagtagtg ttggtaaatt aatgttcttc agacgccatc gacagataga
    14341 cgatcgtcag aaccatgaag atgaaggctt gcagcgtaat gatcaggatg tggaaaatgg
    14401 cccacggcac attcaggatc cactgtgacc accacggcaa cagaccagca atcagaatga
    14461 aaatcagctc accggcatac atgttaccga acagtcgcaa accgagtgaa actggtttgg
    14521 acagcaggct taccccttca aggattaagt tgacaggaat gaacgcccag tgattgaacg
    14581 gctgcagcgt caactctttc gtgaagccgc cgatgccttt cattttgatg ctgtagaaca
    14641 gaatcaggat aaatacgccc agtgccatag acagcgttac gttcacgtca gcagacggaa
    14701 ccacacgcaa tgcaggcaga cccagtacat gttcagcaat gtacggcagc aggtcgatag
    14761 gcagtaaatc catcaggttc atcaggaata cccagacgaa gatcgtcagg gccagcggag
    14821 caatcagctt gcttttgcca tggtacatgt ctttcacgct accattaaca aagccgatca
    14881 ccagctcaat cgcggtctga aacttacctg gcacgccgct ggttgccttt ttggctacac
    14941 tacggaataa aaccaggaac aacagaccca gcaccaccga gaagaacatg gagtcaatat
    15001 tgattgtcca gaaggtggct ggggggttat gtggatccac cagcgagaat gtacgcaggt
    15061 ccagctgaag gttattcagg tggtgtccta tgtaatcctg cggcgtcata ttttctgaag
    15121 ccatgatgcc ttttaccctt tgttgttaat tacagccggt gccagtatct gaaccaccag
    15181 caccaaaacc cacgtaacga tcagcggcaa gaataccgcc tttaaaaccg ccaacgccac
    15241 caccagtaac accaacatcg ccagaacttt gaaagcttcg ccaaatgcga atgtccaggc
    15301 cacccggcct ttcgctggtg tatgcgcctg gtgacgccag gcaaatatca taaacaaaac
    15361 gttaggcaga aagactgcca ggcccccgct tattgcagag acgccccaga aggggtcttt
    15421 gaggctgaac agcaatccac ttgctatcac caccagtaac tgaacgagca gaagcttccg
    15481 agcaacgttt cgactcacga gcgacacaga catcacgttt ttcactcctg ctcccttcga
    15541 ggtatgccgc gtgtcgtata aaactttctt taaggcttag agtcaagcat caaaaagcgg
    15601 tcaaattata cggtgcgccc ccgtgatttc aaacaataag tagccaaaag gtgaataaat
    15661 gtttaaatat ttttccagtg catacaattg cgacttttct gcaaaccctg ttcgatcatg
    15721 aaaaactgta aataacgcgt aaacactggt gataaagcgt gcttcagatc acatattgcg
    15781 catgttcgcg cacagcatat ttatttactt ggcaaatgat gcctttgcaa gtttatgata
//
LOCUS       DAZFJL010000109        15770 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFJL010000109 DAZFJL010000000
VERSION     DAZFJL010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.109"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..842
                     /gene="gatY"
                     /locus_tag="ACHJ8C_003425"
     CDS             <1..842
                     /gene="gatY"
                     /locus_tag="ACHJ8C_003425"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO5553225.1"
                     /translation="TKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAASMHAPVIIAG
                     TPGTFTHAGTENLMALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVMIDASH
                     LPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPVQAREFAE
                     ATGIDSLAVAIGTAHGMYARAPALDFSRLENIRQWVNLPLVLHGASGLSTKDIQQTIK
                     LGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIADCGCE
                     GRA"
     gene            871..2133
                     /gene="gatZ"
                     /locus_tag="ACHJ8C_003426"
     CDS             871..2133
                     /gene="gatZ"
                     /locus_tag="ACHJ8C_003426"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO5553226.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCADDSIPLAPETVAERAAVLCLAAESVATDCQ
                     REQLNYVIGTEVPVPGGEASAIQSVHITQVEDAANTLRTHQKAFIARGLAEALTRVIA
                     IVVQPGVEFDHSNIIHYQAQEAQALAQWIEKTKMVYEAHSTDYQTQTAYRELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSRCLAVIEDVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            2143..2595
                     /gene="gatA"
                     /locus_tag="ACHJ8C_003427"
     CDS             2143..2595
                     /gene="gatA"
                     /locus_tag="ACHJ8C_003427"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO5553227.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALITRE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            2626..2910
                     /gene="gatB"
                     /locus_tag="ACHJ8C_003428"
     CDS             2626..2910
                     /gene="gatB"
                     /locus_tag="ACHJ8C_003428"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO5553228.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHSIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGNIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            2914..4269
                     /locus_tag="ACHJ8C_003429"
     CDS             2914..4269
                     /locus_tag="ACHJ8C_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO5553229.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARNRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIVIGAIYLTGIFMTWRRARGFIKQEKAVLA
                     E"
     gene            4317..5357
                     /gene="gatD"
                     /locus_tag="ACHJ8C_003430"
     CDS             4317..5357
                     /gene="gatD"
                     /locus_tag="ACHJ8C_003430"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO5553230.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPADMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAMSVTAIDISSEKLALAKSFGAMQTFNSREMSAP
                     QIQGVLRDLRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            5463..6236
                     /locus_tag="ACHJ8C_003431"
     CDS             5463..6236
                     /locus_tag="ACHJ8C_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5553231.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(6296..7733)
                     /locus_tag="ACHJ8C_003432"
                     /pseudo
     CDS             complement(6296..7733)
                     /locus_tag="ACHJ8C_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695951.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
     gene            complement(7744..8046)
                     /locus_tag="ACHJ8C_003433"
                     /pseudo
     CDS             complement(7744..8046)
                     /locus_tag="ACHJ8C_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            8042..8591
                     /locus_tag="ACHJ8C_003434"
                     /pseudo
     CDS             8042..8591
                     /locus_tag="ACHJ8C_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397485.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
     gene            complement(8593..9534)
                     /locus_tag="ACHJ8C_003435"
     CDS             complement(8593..9534)
                     /locus_tag="ACHJ8C_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO5553232.1"
                     /translation="MNKEDDIRLDQKVRAAWMYYIAGLNQSEIASQLGTSRPVVQRLI
                     AAAKDEGIVSIGLHHPVANCLDYAQLLKEKYHLTDCNIVPVYSVERTLDSVTFGCYQL
                     MARYLQGDKPTIVGIGSGLTLKKTIRRIDFDSQNTRCVALISAMNEHGQCNYYDDVPL
                     LLARKIQGKYYQWPAPRYAQSREEHQMWSTNRLFRNVSAVACNSNVIFVGIGALGRQS
                     PIFKDGFINEQQLEELTMLGGVGEILGRFIDADGNVVESHINRMITSYDIRESQCPRI
                     AAACGEDKRPAILAALKGGWINGLVTDEHTARWLLTR"
     gene            9742..11110
                     /gene="dalD"
                     /locus_tag="ACHJ8C_003436"
                     /pseudo
     CDS             9742..11110
                     /gene="dalD"
                     /locus_tag="ACHJ8C_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227903.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047813 - D-arabinitol 4-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0051161 - arabitol metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinitol 4-dehydrogenase"
     gene            11124..12587
                     /gene="xylB"
                     /locus_tag="ACHJ8C_003437"
     CDS             11124..12587
                     /gene="xylB"
                     /locus_tag="ACHJ8C_003437"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001692898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO5553233.1"
                     /translation="MYLGIDLGTSEVKALVIDENNEIVATHSAPLTIQRPHPHWSEQS
                     PQAWWEATEYLMSTLREKCGQHWQAIKAIGLSGQMHGAVLLDEAGKVIRPAILWNDTR
                     SALECTELEEIAPELHQVAGNLAMPGFTAPKLLWVRNHEPQHFARIATVLLPKDYLRF
                     KMTGKKVSDMSDSAGTLWLDVEKRDWSDSLLQKCGLTRANMPELVEGCDVSATLEPCV
                     AERWGLTPSVMVAGGGGDNAVSAIGVGAVSPGDAFISLGTSGVLFVVTDAYRPAPQSA
                     VHAFCHVLPNLWHQMSVMLSAASCLQWFCRLVGVTETELLEEIAQLSPAEKASAPMFL
                     PYLSGERTPHNDPDARGMFWGLTHSSQRALMGYAVLEGVSFGIADGLRVLQESGTAIE
                     QCSLVGGGARSPFWAQLLADILNMPVVTHKGGETGGALGAARLACLAAGKPLTAVCEK
                     PEIYQTWHSDAERHQALMQRYQQFNALYNNDLNYRNQ"
     gene            12656..13933
                     /locus_tag="ACHJ8C_003438"
     CDS             12656..13933
                     /locus_tag="ACHJ8C_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbtT/DalT/CsbX family MFS transporter"
                     /protein_id="HFO5553234.1"
                     /translation="MSLTNKQWLGLPLNLVWGYIAIAIFMTGDGFELAFLSHYIKALG
                     FTPAEASFAFTLYGLAAALSAWVSGVVAEIITPQKTMFIGFVLWCIFHVLFLVFGLGQ
                     ANYALILLFYGIRGLAYPLFLYAFIVAVVHNVRSDSTSAALGWFWAVYSIGIGVFGSY
                     IPSFTIPVIGEMGTLWLALGFCVTGGILALVSMRKVQTPHHMQNLTTKEKFSELGRAV
                     TLLYTNRNILLSSIVRIINTLSLFGFAVIMPMMFVDELGFTTSEWLQVWAVFFFTTIF
                     TNIFWGVLAEKIGWMKVVRWFGCVGMALSSLAFYYMPQYFGHNYWMAMVPAVALGIFV
                     AAFVPMAAVFPALEPKHKGAAISVYNLSAGLSNFLAPAIAVVLLPYFSTIGVVIAYTT
                     LYIIAFIICPFIRVEQPGFSTQKLRAGEAATVS"
     gene            13978..14619
                     /locus_tag="ACHJ8C_003439"
     CDS             13978..14619
                     /locus_tag="ACHJ8C_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFO5553235.1"
                     /translation="MFNCKAVIFDMDGVIIDSEGLWRQAQQGALARWGASVSDEECIQ
                     LTKGKRLDEIARTWCEYCHLSITPTELEAEIRRLITTLIAEKGEAMPGVKNVLTFLCQ
                     SGYQLALATSSSYQVIDAVLTKLKIEHCFSVICSADDESHGKPHPAVYLSALRKLRLP
                     AADCLVIEDSLSGFKAAQAAGIKTIVVAEDFQQPCFNSAVVGYASLEELMATG"
     gene            complement(14700..15599)
                     /gene="yegS"
                     /locus_tag="ACHJ8C_003440"
     CDS             complement(14700..15599)
                     /gene="yegS"
                     /locus_tag="ACHJ8C_003440"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO5553236.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAVRFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDEIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEIIPTLVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         3787 a         3703 c         4171 g         4109 t
ORIGIN      
        1 cgacaaagca gatgctgaac aacgcgcagc gcggcggtta tgcggttccg gcattcaata
       61 ttcacaatct cgaaacgatg caagtggtgg tggaaaccgc tgccagcatg catgcgccgg
      121 tcatcatcgc cggaacgccc ggcacattta ctcatgccgg tacagaaaat ctgatggcgc
      181 tggtcagcgc gatggcgaag caatatcacc atccactggc gattcatctc gaccatcaca
      241 cgaaatttga cgatatcgct cagaaggttc gttctggcgt gcgctcagtc atgattgacg
      301 cctcgcattt gccttttgcg caaaatattt cacgggtcaa agaggtggtg gatttttgcc
      361 atcgctttga tgtcagcgtc gaagcggagc tggggcaact tggcggccag gaagatgatg
      421 tgcaagtcaa tgaagccgat gctttttata ccaaccccgt tcaggcgcgt gaatttgccg
      481 aggcaaccgg gattgattcc ctggcggtcg ccatcggcac ggctcacggt atgtatgcca
      541 gagcaccggc gcttgatttt tctcgactgg agaacattcg ccagtgggtg aatttaccgc
      601 tggtgctgca tggcgcgtca ggattatcga ctaaggatat tcagcaaacc atcaaactgg
      661 ggatatgcaa aatcaacgtt gcaacggagc tgaaaaatgc cttctcgcag gcgttaaaaa
      721 attacctgac cgagcacccc gaagcgaccg atccccgtga ttatttgcag tcggctaaat
      781 ctgcaatgcg cgatgtggtg agcaaagtga ttgccgattg tggctgcgag ggcagggcat
      841 aacgcacctg ccatttaaca aggaaaaaac atgaaaacgt taattgcccg gcataaagct
      901 ggtgaacata ttggcatatg ttcagtctgt tctgcacatc cgttggttat cgaagcggcg
      961 ctggcatttg atcgcaacag cacgcgtaaa gtgctgattg aagcaacgtc aaaccaggtc
     1021 aatcaatttg gcggttatac cggaatgaca ccggcagact ttcgcgaatt tgtttttgcg
     1081 attgccgata aagtcggatt tgctcgtgag cgtattattc tcggcggcga ccatctggga
     1141 ccaaactgct ggcagcaaga aaatgcggat gcggcgatgg aaaaatccgt cgagctggta
     1201 aaggcatatg ttcgtgccgg cttcagtaaa attcatcttg atgcgtcaat gtcctgcgct
     1261 gacgattcca taccgttagc gccagaaacg gttgctgaac gtgccgctgt gctttgtctg
     1321 gcggcggaaa gtgtggcgac tgattgccag cgtgagcaac tgaactatgt cattggcact
     1381 gaagttccgg ttccgggcgg tgaggccagc gccattcagt cagtacatat cacccaggtt
     1441 gaagatgccg ccaatacttt acgcacgcat caaaaagcgt ttattgcccg cggtctggca
     1501 gaggcgttaa cacgcgtgat tgccatcgtg gtgcagccgg gtgtggaatt tgaccatagc
     1561 aatattattc attatcaggc gcaagaagct caggcgctgg cgcaatggat agaaaaaacc
     1621 aaaatggttt atgaagcgca ttccaccgat taccagactc agacggccta tcgggaatta
     1681 gttcgcgatc attttgcaat tttgaaagtc ggtccggcat taacctttgc tttacgtgag
     1741 gcgatatttg cgctggcaca aattgagcag gaactcatcg cacccgaaaa tcgtagccgt
     1801 tgtctggcgg taattgaaga tgtgatgctt gacgaaccac aatactggaa aaaatattat
     1861 cgcacaggtt ttaacgattc attactggat attcgttata gcttgtcgga tcgtattcgt
     1921 tattactggc cgcatagccg gattaaaaat agcgtcgaaa cgatgatggt gaatctggaa
     1981 ggcgtggaca tcccactggg catgattagt cagtatcttc cgaaacaatt tgaacgcatt
     2041 cagtccgggg aattatcagc aataccgcat cagcttatta tggataaaat ttatgatgtt
     2101 ttgcgcgcct atcgctacgg ctgtgcggaa taaggacgat atatgactaa cctgtttgtt
     2161 cgtagcggaa tttcttttgt cgatcgtagc gaagttttaa cccatatcgg taatgagatg
     2221 ctcgcgaaag gtgtggttca tgatacctgg ccacaggcat taattaccag agaagcagaa
     2281 ttccctaccg ggataatgct tgagcagcac gccattgcaa taccgcattg tgaggcgatt
     2341 catgctaagt cgtcagccat ttatctgtta aggccaacaa ataaagttca ttttcagcaa
     2401 gcggatgatg ataacgacgt ggcggtatcg ttggttattg cgttgattgt ggaaaatccg
     2461 cagcagcaat tgaaactttt acgctgttta tttggcaagt tacaacagcc cgatatcgtc
     2521 gagacactaa tcactcttcc tgaaacccag ttaaaggaat acttcacaaa gtatgtttta
     2581 gattcagacg aataaatccc tctgtaacaa taaaaaggac tatttatgaa acgcaaaatt
     2641 attgtcgctt gcggaggcgc ggttgcgacc tctacgatgg cggcggaaga aattaaagag
     2701 ttgtgtcaga gtcatagtat tcctgttgaa ttaatccagt gtcgggttaa tgaaatagaa
     2761 acctatatgg atggcgtgca tttgatatgc accactgcca gggtggatcg tagttttggc
     2821 aatattccgt tagttcacgg catgcctttt gtttctggtg tcggtatcga agcattacaa
     2881 aataaaattc tgactatctt acaggggtga cctatgtttt cagaagtcat gcgttatatt
     2941 ctcgacctcg gccctacggt gatgctgccg attgtcatca ttattttttc taaaatatta
     3001 ggcatgaagg caggcgattg ctttaaagcg ggtctgcata tcgggattgg ctttgttggc
     3061 attggccttg tgattggctt aatgctggat tccattggcc cggcggcgaa agcgatggcg
     3121 gaaaatttcg acctgaatct gcatgtggtc gatgtcggtt ggccgggctc ttcacccatg
     3181 acctgggcgt cgcaaattgc gctggtggcg attccgattg cgattctggt taatgtggcg
     3241 atgttactga cccgtatgac gcgggtggta aatgttgata tctggaatat ctggcatatg
     3301 accttcaccg gcgcgttgct gcatctggca accggttcat ggatgatagg gatggcgggt
     3361 gtggtaattc acgcggcgtt tgtttataag ctcggcgact ggtttgcccg cgatacccga
     3421 aatttctttg agctggaagg cattgctatt ccgcacggta cgtcggcgta tatggggccg
     3481 attgcggtgc tggtcgatgc tatcatcgag aaaatcccag gcgttaaccg aattaaattt
     3541 agcgccgatg atattcagcg caaatttggc ccttttggcg aaccggtcac cgtgggtttt
     3601 gtgatggggc tgattatcgg catcctcgcg ggttacgatg tcaaaggtgt attgcagctg
     3661 gcggtaaaaa cggcggcagt tatgctgctg atgccacggg tgattaaacc catcatggat
     3721 ggtttaacgc ccatcgctaa gcaggcccgt aatcgtttac aggcgaagtt cggcggtcag
     3781 gagttcctga ttggccttga tccggcgttg ctgctgggac atacggcggt ggtatcggca
     3841 agcctgattt ttattccact caccatttta attgctgttt gtgtgccggg taatcaggtg
     3901 ctgccgtttg gcgaccttgc caccatcggc ttctttgtgg cgatggcggt cgcggtgcat
     3961 cgtggaaatc tgttccgcac cttaatctcg ggtgtcatca ttatgagcat caccctgtgg
     4021 atcgcgacgc aaactattgg tttgcacacc caactggcgg ctaatgcggg cgcgttaaaa
     4081 gccgggggta tggtggcttc aatggatcag ggcggttctc ccattacctg gttactgatt
     4141 caggttttct ccccgcaaaa tattcccggt ttcattgtta tcggcgcaat ttatcttacc
     4201 ggtattttca tgacctggcg tagagcgcgt ggttttatta aacaagagaa agccgttctc
     4261 gcagaataat ttttacctga gggggaggtt atccccctta acaatcagga gtttttatga
     4321 aatcagtggt aaatgatact gatggtatcg tgcgcgtagc agaaagcgtc attcctgaaa
     4381 ttaaacatca ggatgaggtg cgggtaaaaa ttgccagctc gggcttatgt ggttccgatt
     4441 tacccaggat atttaaaaat ggtgcacatt attatccaat aacgttaggc catgaattta
     4501 gtggctatat tgatgcggtg ggatccggtg ttgatgattt acaccccggc gatgcggttg
     4561 cctgtgtgcc gttattaccc tgttttactt gtccagagtg tctgaaaggg ttttattccc
     4621 agtgcgcaaa atatgatttt attggctcac ggcgtgatgg tggatttgcc gaatatattg
     4681 tcgttaagcg gaaaaatgtc tttgctctac ctgcggatat gcctattgag gatggcgctt
     4741 ttattgaacc gattaccgtt ggcctgcatg cgttccattt agcgcaagga tgtgagaata
     4801 aaaacgttat tattattggt gccggaacca ttggcctgct ggcaattcag tgcgctgtcg
     4861 cgctgggagc aatgagtgtg acggctatcg acattagctc agaaaaactg gcactggcaa
     4921 aatctttcgg tgcgatgcaa acatttaaca gccgtgaaat gagcgcgccg caaatacagg
     4981 gcgttttacg cgatctgcgc tttaatcagc ttatcctcga gacggctggc gtcccgcaaa
     5041 ccgtcgaact ggcggtagag attgccggtc ctcatgccca actggcgctg gtaggcacgt
     5101 tgcatcagga tctgcattta acatctgcaa cgtttggcaa aatattgcgt aaagagctga
     5161 cggttatcgg cagttggatg aactattcca gcccttggcc ggggcaggag tgggaaacgg
     5221 cgagccggtt gctgacagaa cgtaagttaa gcctggagcc attaatcgct caccgtggaa
     5281 gctttgaaag cttcgcccag gcggtgcgtg acatcgctcg taatgccatg ccgggcaaag
     5341 tgttgctcat tccctgaaac cgcgggccag cgtgatgcag gcccggtatt gtgcaaaaca
     5401 gatcattcac caatggtccc ccttcgttta cactagccac aattgaatat gggtaaatga
     5461 cgatgaattc attcgagcga aggaataaga tcatccaatt agtgaatgaa cagggaaccg
     5521 tgcttgttca ggatctggcg ggagtatttg ctgcctcgga agcgacaatc cgtgccgatt
     5581 tgcgctttct cgaacaaaaa ggcgtggtta cgcgctttca tggcggtgcg gcgaaaataa
     5641 tgtccggtaa tagcgaaacc gagacccagg aagtcgggtt taaagagcga tttcagctcg
     5701 ccagcgcgcc gaaaaacaga atagcgcagg cggcagtcaa aatgatccac gaagggatga
     5761 ccgttattct cgacagcgga agtacgacaa tgcttatcgc cgaaggatta atgaccgcca
     5821 aaaatatcac ggtgattacc aacagtctcc cggctgcgtt tgccctttcc gaaaataaag
     5881 acattactct ggtcgtctgc ggtggcacgg tccgccataa aacgcgatct atgcatggat
     5941 ctattgctga acgttctctg caggatatta atgccgattt aatgtttgtg ggtgctgatg
     6001 gtattgatgc ggttaatggt attacgacct ttaatgaagg ttattctgtt agcggtgcga
     6061 tggtcaccgc tgctaataaa gtgattgccg ttctcgattc atcgaaattt aaccgtcgcg
     6121 gttttaatca ggtactgcca atcgaaaaaa ttgacattat tattaccgat gatgctgtgt
     6181 ctgaggtgga taaactggca ttgcagaaga cacgggtaaa attaattacg gtgtagttta
     6241 ttatcaattt tgtgggcaat aaaaccggga acttcaagga gccccggttt tttatttact
     6301 cccgacagtt ttttgccagc gcctgtaatt gctgataagc ctcataccgc tgcttatgca
     6361 gcgtctggta attttgttgt ggataatatt gcgtatccac atgggtaaat tgctgcatgg
     6421 catcactcac cgaaggtgaa acttttccgg caacagctcc cagaattgcc gagcctaata
     6481 ataccggttc gctacacttc gtggtaatga ccggcagacc atactgccag aatttgccgc
     6541 accagtgggt gctgaccggc accgccgcaa atgacgatat ttttattgac tacgcccaga
     6601 gcggattgcg catcagaatt tgtcgcagac catagacgat gccacataac ccggcaacat
     6661 acagaacgag taaattatcc tgatcgcact ccatacccag cccgcaaatc acagctctgg
     6721 catgaggatc ggcgaagggg gcgcgattac cgagaaattc cggcaccaca tgtaaacctt
     6781 ttgccagcgc cacggcatcg gacggttgcg ccgtttttgc caggatttgg tcggcaagcc
     6841 ataccagtaa tggttgtccg gcctgctgag cttgtttatt tgcttcatca aacgcaggat
     6901 ggaacgacag taactgatcg atagccgcac cggcagcact ttgtccgcct tcgattaatc
     6961 ataacccagg aaccataacg ttatagtact gtccccagat gccgggaaca aaaagcggat
     7021 cggtaacgga ggccattgcc acagggcgtt cctggcgatg caatatgctg accgatacgt
     7081 gcaaattctt catcagctaa ctctgtcagg ccaatggtgt ggaagtagtc cggatcacag
     7141 cgattttcat gagccagcca cgtccatttg caggtcacgg tacaggccga acgagccagg
     7201 tcccccgtgg cgcgccaggt cagaaaatca gccagatcga agaaatatcc agcacgatcg
     7261 aagacttcag gcatattttc tttaagccac aggagcttcg gtgtttccat ttccggtgaa
     7321 atgatcccgc cgacgtattt cagcaccggg tgatgtgtgg cattgattcg ctttgcctgg
     7381 ttggtagcac gatgatccat ccagacaatg atgttttgct gcgcttcacc ttccgggctg
     7441 accggcaacg gttcaccatt tttatccagc accaccagag agcaggtagc atcaaagcca
     7501 atacccgcga cactctgtgc agagacatcc gcttttgaca atgcctcccg aatgcttgaa
     7561 caaaggacct gccagatttg ctgactggac tgttcgacgc ggctaccgct gcgtcgggtg
     7621 gtcgtgattt tgtgtgccgc gtgagcaagt aatgtgccct ggagatccaa agccccagcc
     7681 cggacgctgc ctgaacccac atcgacaccg atgacagttt tggttgcagt catagtaatg
     7741 cccttacaga tcaacgctgt ttggcaggat caccagatcg cgcacggtga cattttttga
     7801 acgggtgacc atgaacagca ccgactccgc tacttcgatc ggctgcatca ggctgccgtt
     7861 tgccagagcc tcatccattt tggctttcgg ccagtcgtcg tgcagagcgg taaccaccgg
     7921 acccggcaac accgcgccaa cgcggatgcc atattgtgaa acctggcggc gagttgtgtg
     7981 gacgaaggcc tgaaccgcaa attttgacac ggtgtagata ggttcccaga taactggcac
     8041 gacaccgtcg attatttctg ataactacac gggcgcgaaa ttattgaccg aacgcatttt
     8101 cgctaatagc cagaaacgtt acagcaaatc aggcgcgctg gtgtttgtcg gcgggcgtat
     8161 cagtgataac aataccgctg agcgtttacg cggttttgtc gatgctcatc aggcgattgg
     8221 gttaactgtt ccggataatc ataaactggt taccggctac tcttccgcac gagtggaagg
     8281 attattgaaa gacgtttttc actcctcagc gatgccgctt cacgggctgt ttgtcaactc
     8341 aaccatttct cttgaaggcg ttgtgcgctg gctggcgcaa acgggacaga cgggggccaa
     8401 tcaaccgccg atgggctgtt ttgactggga tccgtttgtt tctctgctgg ggcatgatat
     8461 cgacatggtg cgtcaggatg tgcccagcca tgctggagaa ggtgtctgag attatagatt
     8521 ccggtgacac cacggtgcgg cgcattgaga tctcaccgga attgctcccc agtctgaatg
     8581 tggttgcttg acttagcgag tcaataacca acgagcggtg tgttcatcgg tcaccaggcc
     8641 gttgatccat ccgcctttta acgccgcgag aatagccggg cgtttatctt caccgcaggc
     8701 ggcggcaatt cgcggacact gactttcgcg gatatcgtag ctggtgatca tccggttaat
     8761 atggctttcc accacattgc cgtcggcgtc gataaagcgc ccaagaattt ccccgacacc
     8821 accgagcatc gttaactctt ccagttgctg ttcattgata aagccatctt tgaagatcgg
     8881 gctttgccga cctaatgcac cgatgccgac gaatataaca ttggaattac aggcgacagc
     8941 agagacattg cggaacagtc ggttggttga ccacatttga tgttcttccc gtgactgggc
     9001 atagcgcggg gcaggccatt gataatattt gccctgaatt tttctcgcca gcagtagtgg
     9061 aacatcgtcg taatagttac attgtccatg ttcgttcatg gcgctgatca gtgcaacgca
     9121 acgggtattt tggctatcaa aatcaatccg tctgatggtt ttctttaagg ttaagccaga
     9181 accgataccg acaatagtcg gtttatcacc ctgtaaataa cgcgccatca gttgataaca
     9241 accaaatgtc acgctatcga gggtacgttc aacactgtat accggcacaa tattacagtc
     9301 ggttaaatgg tacttttcct ttaaaagttg cgcgtaatcg agacaattcg ccaccggatg
     9361 atgtaatcca attgacacaa tcccttcatc tttagcagct gcaattagcc gctggacaac
     9421 cggacgagac gtccctaact ggctggcgat ttcgctctgg ttaagtccgg cgatgtaata
     9481 catccacgcc gcccgtactt tttgatcgag cctgatgtca tcttctttgt tcacgtttcc
     9541 ctacctcaag attgtgtgcg gaatggaaaa attccacaac agatagcgtc attttggcga
     9601 cagctctgcc atgaggcaag tttttacctg gttttggtca tttgtaattt tatagatcat
     9661 ttgccctttt gtgatctgta gctcttattc gaatcaaatg ttcatttaca gtgcagccat
     9721 aacgtcaatg gagaatagac aatgaaagaa aaatacacat ggctgcatat tggtttaggt
     9781 tctttccacc gcgcacacca ggcgtggtat ctgcatcgcc tgctggtggg ggggggatgc
     9841 gcgctggagt attgcagcgg gcaatattcg taacgatgcc gaacatgttg tgcaggcgtt
     9901 gatgacgcaa aaaggccgtt atgtgctgga aacggttagc ccggaagggg agagcgtata
     9961 tgaagaaatc acctcaattc agaaattgtt gccctggcag gtcgatttac aaccgctgat
    10021 tgccgaaggt gctgacccga aaaccaaagt gattgccttt acggtgaccg aaggtggcta
    10081 ctacctgaac accagccata agctggaagt tacaaaccct gatttagctg ctgacctcaa
    10141 gggcgaatgc agaaccattt atggtgttat tacccgcatt cttgaagcac gtatggcaaa
    10201 taacgccgga ccgttgactc ttctgaactg cgataacgtg cggcataacg gcgagcgttt
    10261 ccatgatggc ctggtggagt tcttacaact tacgggtaaa cagaccgtta tcgactggtt
    10321 aagcgccaat gcgacctgcc cgaatactat ggtggatcgt attacgcccc gtccgtcgac
    10381 agaacttccg gaacgtatca aagcgcaaac cggtatgagt gacaaagccc cggtcatggg
    10441 cgaaaccttt atccagtggg tagtggaaga taatttccgc gatgtgcgcc cggcactgga
    10501 gaatgtcggc gtggagctgg tggattcggt tattccttat gaagaagcga aaattcgtat
    10561 tcttaactct tctcatagct gtatcgcctg ggcaggtacg ctggttggtc agaaatatat
    10621 ccacgaaagt acgctgactg attttattta tcagattgcc gatcgctacg taacggaaga
    10681 tgttattcct tgtctgggtg acaatggcat tgatcttgaa acttaccgtg atgtggtgct
    10741 caggcgtttt accaacccgc atattcagga cactaaccag cgcgttgccg ccgatggctt
    10801 ctctaaaatt ccggcgatga ttgcgccaac tttgcgtgaa tgttaccagc gcggcgtccg
    10861 tccaaatgct accgctatgt taccggcatt gttctatgtg tttatgcagc agtggcatct
    10921 cggcaaactg ccgtatgaat accaggacgg tattcttgat gcgccagcgg tacatgcgat
    10981 gttcgaatcc gcagacccga ttgccgttta tgccagcgac aaggcactgt tcggcgattt
    11041 aaccgactgt gccgattttg ccgcgctgct gcgcgaaaaa attgctgacg tctacgcgct
    11101 gattaattaa ccaggaggcg gttatgtatc tgggtatcga tctgggaaca tcggaagtta
    11161 aggcgctggt cattgatgaa aacaatgaaa tagtcgccac acatagcgcg ccgctgacta
    11221 ttcagcggcc ccatccacac tggtcagaac aatcgccgca ggcctggtgg gaggcgacgg
    11281 aatacctgat gtcaacgcta cgggaaaaat gcggtcagca ctggcaagcg attaaagcga
    11341 ttggcctttc tggtcagatg cacggcgcag tgctgctgga tgaggccggg aaggtgattc
    11401 gtccggcgat tttatggaac gatacccgca gtgctcttga gtgtacggaa ctggaagaga
    11461 ttgcaccaga gctgcatcaa gtggctggca acctggcgat gcccggattt accgcgccga
    11521 aactgttgtg ggtacggaac catgaaccgc agcattttgc acgtattgcg acggtgttac
    11581 tgccgaaaga ctatttgcgc ttcaaaatga ctggcaaaaa agtctccgat atgtcggatt
    11641 cggcgggcac gttgtggctc gacgtggaaa aacgtgactg gtcagactcg ctattgcaaa
    11701 agtgcggcct gacgcgggca aatatgccgg aactggtgga aggttgtgat gtctctgcca
    11761 cgctcgaacc gtgtgtagcc gaacgctggg ggctgacgcc ttcggtgatg gtggctggtg
    11821 gcggagggga taacgcggtt agcgccatcg gtgtgggcgc agtatctccc ggcgatgcgt
    11881 ttatttctct ggggacatcg ggcgttctgt ttgtggtgac tgatgcttat cgtccggccc
    11941 cgcaatcagc cgtccacgca ttttgccatg tgctgccgaa tttatggcat cagatgagcg
    12001 tcatgcttag cgccgcaagt tgcttgcaat ggttttgccg tctggtgggc gttaccgaaa
    12061 cagaattact cgaagagata gcccagcttt cgccagcaga aaaagccagt gccccgatgt
    12121 ttctgccata tctttccgga gaacggactc cccataacga cccggatgcc agagggatgt
    12181 tctggggact gacccattcc agccagcgag cactgatggg ctatgccgta ctggaaggtg
    12241 tgagttttgg tattgctgac gggctgcgtg ttctgcaaga gagtggaacg gctattgaac
    12301 aatgttcgct ggtgggggga ggggcgagaa gtccgttctg ggcgcaactg ctggcggata
    12361 ttctcaacat gccggttgta acgcataaag gtggggaaac cggtggtgcg cttggcgcgg
    12421 cgcgtctggc ttgtctagcc gcaggtaaac cgctgacggc ggtctgcgaa aaaccggaaa
    12481 tctaccaaac ctggcacagc gatgctgaac gccatcaggc tttgatgcaa cgttatcaac
    12541 agtttaacgc gctatacaac aacgatctta actatcgcaa ccaataacaa ctgtcattaa
    12601 tgctctccgg tggtgacacc ggagcggcgt tttgtaccct acaaacgagg tcactatgtc
    12661 gttaaccaat aaacaatggc tgggattacc tttaaatctg gtctggggat atatagcgat
    12721 tgctatcttt atgaccggcg atggttttga actggcattc ctatcgcact atattaaggc
    12781 gctgggcttt acgcctgcgg aagcttcatt tgctttcacg ctgtatggtc tggcggcggc
    12841 actctctgca tgggtatccg gcgtggtggc ggaaattatt acgccgcaaa agaccatgtt
    12901 catcggtttt gtactgtggt gtattttcca tgtgctgttt ctggtcttcg gtctggggca
    12961 ggcgaactat gcattaattc tgctgttcta cggcattcgt ggtctggcat atcctctctt
    13021 cctttacgcc tttatcgttg ccgttgtgca taacgtgcgc agcgacagta ccagtgccgc
    13081 gctgggctgg ttctgggcgg tttactccat tggtattggc gttttcggta gttacattcc
    13141 cagctttacc attccggtta tcggcgaaat gggcaccctg tggctggcgc tgggattttg
    13201 cgtaactggc ggcattcttg cgttggtttc gatgcgtaaa gttcagactc cgcatcatat
    13261 gcaaaatctg acgaccaaag agaagttcag cgagctcggc cgtgctgtca ccttactcta
    13321 caccaaccgc aacattttgc tctccagtat tgtgcgaatt atcaatacat tgtcactgtt
    13381 tggctttgcg gtgattatgc cgatgatgtt tgtcgatgaa ctgggcttta ctacgtccga
    13441 gtggctacag gtgtgggcgg tcttcttctt cacgacgatt tttaccaaca ttttctgggg
    13501 cgtactggca gagaagattg gctggatgaa agttgttcgc tggttcggct gtgtgggaat
    13561 ggcactttcc agtctggctt tctactacat gccgcaatac ttcgggcata actattggat
    13621 ggcaatggtg cccgccgttg cgctggggat ctttgttgca gcgttcgtgc cgatggcggc
    13681 ggttttcccg gcgctggagc caaaacacaa aggcgcggcg atttcggttt ataacctctc
    13741 tgccggtctg tctaactttc ttgcccctgc gatagccgtg gtgctgttac cttatttcag
    13801 tacaattggt gtggttatag cttatacaac cctgtacatt atcgcgttta ttatttgccc
    13861 gtttattcgg gttgaacaac caggattctc aacccagaaa ttaagagcag gtgaagccgc
    13921 gacggtttcc tgatggaggc aagcctggtg taatgccagg ctttaatgtg aggaacgatg
    13981 tttaattgca aagcagttat ttttgatatg gacggcgtca ttattgattc ggaaggatta
    14041 tggcgtcagg cgcaacaagg agcgctggca cgatggggtg ccagcgttag cgatgaagag
    14101 tgtatccaac tgacgaaagg gaaaaggctt gatgagatag cccgaacctg gtgcgaatac
    14161 tgtcatctgt ctattacgcc cacggaactg gaagcggaga tccgtcgcct gataaccacg
    14221 ttaattgctg aaaaaggcga ggccatgccg ggggtaaaga atgtgctcac ttttttatgc
    14281 cagtcaggat accaactggc actggcgacc tcttcatctt atcaggtgat tgatgcggtg
    14341 ctgacaaaac tcaaaattga acactgtttt tcggtgattt gcagtgctga cgacgaaagc
    14401 cacggcaaac cacatccggc agtttacctc tccgcactac gaaaattacg cctgcccgcc
    14461 gcagattgtc tggttataga agacagtctg agtggtttta aagctgcaca ggcagcggga
    14521 attaaaacga ttgttgtcgc cgaggatttt caacagccgt gctttaactc tgcggtagtc
    14581 gggtatgcat cacttgaaga gttaatggca acggggtgac tgcatgaaaa aattgtaagc
    14641 gcagaagaat ccagggatga tgccggcctg cggatcctgc ttctgcgttt gttttgtact
    14701 taccttaaca atggacaatc tggtggtaat cgacaacgca acgccgccgg aagtatttca
    14761 atatggaagt tttgcccact caacggttcg ccatcaagat taaaagtgat ttcgtgtggg
    14821 gcttgtatat caaaccacga cgaagctcct tcgataatat tcgggttatc ttcgtcagat
    14881 tttaaggttg atacgagagt cggaataatt tcatcgccgg taaaaatgcg cagttgcagc
    14941 aagccatcgt taattaatgc gttcgggcac aattgttgac cgccaccggc ctgacgcccg
    15001 ttaccaatac caatgaccag ggcgtcacct tgccagtgaa agttttcacc gcggatttca
    15061 caacggtccg gttgcagagt gtccatgcgc attaagccat gaatgatgta agagacgcca
    15121 cccagcgcgg cttttaattt ttccggcgtt tctgtggtaa tacgcgtccc aaacccgcct
    15181 gtcgccatat taataaaaca ggtttgtttg ttgacctgcg ccatatctat cgcaatggcg
    15241 ttaccggcaa ttgccagttt cagcgcctta tccagtgcct caggaatccc tacactagtg
    15301 gcaaaatcat tggcagttcc taatggtaaa attcccagcg cgggtatttc atccccctca
    15361 cattgaatca atgccgtaga aacttcatta atggtgccat cgccaccacc ggcaatcact
    15421 gttgcgacac ctaacttccg ggcctcctct acaaatcgta cggcatcacc tttctcccag
    15481 gtaacccgca catggatcgt cattccttcc tcacgcaaca gcataattgc ttcgcgcaag
    15541 ggtagattgt cagtactttt gccattaaga atcagtaagc tggcgggaaa ttctgccatg
    15601 atcgtatgtg cctttatgat tggtctgtag atagtgtaga gcagaaaaga aaaggtggag
    15661 tcagaacagg atgaaagtcg gaggataaaa agtcagttca ggtatgggga ttcagtgctt
    15721 accaccagga gcttacaatg gggacagcga atattaatgg tagccactga
//
LOCUS       DAZFJL010000110        15572 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZFJL010000110 DAZFJL010000000
VERSION     DAZFJL010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.110"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            12..1712
                     /locus_tag="ACHJ8C_003441"
     CDS             12..1712
                     /locus_tag="ACHJ8C_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HFO5553237.1"
                     /translation="MIIGIDLGTTNSLAAVWRNGQSELIPNALGKFLTPSVVCVDEDG
                     MVLTGEAARDLQLIKPQNCASNFKRMMGTSKTLKLGGREFRAEELSSLILRQLKEDAE
                     NYLCEEVTEAVISVPAYFGDMQRKATKAAATMAGLNVERLINEPTAAALAYGLHNKDD
                     EHQFLVFDLGGGTFDVSILELFDNIMEVRASAGDNFLGGEDIVDILIDAYCSRRDLPE
                     NIEWREPTLQRHLRIEAERVKRVLSVRDEATFSVEIEGRRYYWHLTTEKFEFLLQTFF
                     ERIHMPLERAIRDAKINISQLDQVVLVGGTTRMPLIRKLVTRLFGRIPAMHLNPDEVI
                     AQGAAIQAALKARHSELRDVVVTDVCPYTLGVDTSKSLGHTRESGYFAPVIERNRSIP
                     CSRVRAFYTAHDQQTEVNFKIYQGESRMVADNIFLAELSVNVPPKPAGDVRIDVRFTY
                     DINGILDVDISVPLTGAKNSLVIEQNPGALTAEQIQQSLSKLSLLKIHPRDEQINQAF
                     IARLDNLYQLTLAETRDWISNCSRHFSYLLEKQDPDQLADFRTKVEPTLDSLERERLQ
                     "
     gene            1709..3658
                     /locus_tag="ACHJ8C_003442"
     CDS             1709..3658
                     /locus_tag="ACHJ8C_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFO5553238.1"
                     /translation="MNCWEILDLQPGAEERDIKRRYAQLVKNCRPEDDPAAWQQLHDA
                     YEQALRYDDEDQWQEEEEEQPINMPRGIVYPRPVMTFRAPNTFSGHPLPDVAVKPEGE
                     FTWQAVLDMLCEENEATPEHAPMQLSKALSFLEKKDLSSRMCFEEALLLYLHRVFRPL
                     LTLAAAQTFRWHLVAGSRQPVIQTEINESCLLYYRIEKEIASYFYSTMSPSEELENGK
                     ALLSSYQQLHEDDRSHKWFKVALLNLINKYDLSDDYLSLIIELIGWSFQPDPHQFCWQ
                     VSEQFYVLFNLHKTGYEKILFALNLEQGKYQQSKKLTSYKRAAQQGNSVFQNDLGAMY
                     YIGEIIKKDFVQAKYWFEKSAGQGNNDALLNLALMYRDGKGVNKNPQKAISLYLNAAN
                     KNHPLAQHSLACMYRDGEGVEVDDEQAFKWCQKSAEQGYAEAQYHLATMYIDGRGVDV
                     DYQQVVHWLNLSAEQKNSKAEYTLGYMYYKGTEIPQDMTMAINLLKAAADHGDIDAAL
                     VLGQLYVHGREGYLPKDIHQAIAYLNQAQEGGEEQSAEILGMIYYQGFKEIAENSALS
                     FNMFFKAAYAGIKRAQRMLSIMYMEGDGTDKNYILSWAWGIISEAYHDLESNDWLPEV
                     RESLSLQDLSRAQRMARILKKRYQL"
     gene            3737..4039
                     /locus_tag="ACHJ8C_003443"
     CDS             3737..4039
                     /locus_tag="ACHJ8C_003443"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFO5553239.1"
                     /translation="MLAEMYLYGTKDTIKDENHALHWYEKAARLGATEAQHQTAAMYA
                     QGTGTKIDNKQAWMWLTIAGNNNPWIEKDALRCLMSEQDIQDAEQTAANCKRLLRI"
     gene            complement(4095..5438)
                     /gene="gdhA"
                     /locus_tag="ACHJ8C_003444"
     CDS             complement(4095..5438)
                     /gene="gdhA"
                     /locus_tag="ACHJ8C_003444"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO5553240.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            5674..5946
                     /locus_tag="ACHJ8C_003445"
     CDS             5674..5946
                     /locus_tag="ACHJ8C_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO5553241.1"
                     /translation="MSRALIAVVLAFPLITLANPHYHPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            complement(5912..6318)
                     /gene="nudG"
                     /locus_tag="ACHJ8C_003446"
                     /pseudo
     CDS             complement(5912..6318)
                     /gene="nudG"
                     /locus_tag="ACHJ8C_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
     gene            6405..7025
                     /locus_tag="ACHJ8C_003447"
     CDS             6405..7025
                     /locus_tag="ACHJ8C_003447"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO5553242.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVAILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(7034..8341)
                     /gene="ynjE"
                     /locus_tag="ACHJ8C_003448"
     CDS             complement(7034..8341)
                     /gene="ynjE"
                     /locus_tag="ACHJ8C_003448"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO5553243.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNEWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKFYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(8408..9061)
                     /locus_tag="ACHJ8C_003449"
     CDS             complement(8408..9061)
                     /locus_tag="ACHJ8C_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO5553244.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRHWVFSEIRAMAIPVVQVTHDLQDVPPDSPVLDMAQWSENYNKLR"
     gene            complement(9061..10596)
                     /locus_tag="ACHJ8C_003450"
     CDS             complement(9061..10596)
                     /locus_tag="ACHJ8C_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO5553245.1"
                     /translation="MATPLRYALIFLLWAMMAVIYAPLIPAALTLISLALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQLIVLDSLGYSRWQCLNWLLLPSVASALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWRGWRRTIPRVDGVRKP
                     ATPLLPGNTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVAAFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPALIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(10569..11735)
                     /locus_tag="ACHJ8C_003451"
     CDS             complement(10569..11735)
                     /locus_tag="ACHJ8C_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5553246.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEATAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPTALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKANTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(11745..12293)
                     /locus_tag="ACHJ8C_003452"
     CDS             complement(11745..12293)
                     /locus_tag="ACHJ8C_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO5553247.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRARLATHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(12293..13000)
                     /locus_tag="ACHJ8C_003453"
     CDS             complement(12293..13000)
                     /locus_tag="ACHJ8C_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO5553248.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAVLLAWALLPSVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(13014..13691)
                     /gene="ydjY"
                     /locus_tag="ACHJ8C_003454"
     CDS             complement(13014..13691)
                     /gene="ydjY"
                     /locus_tag="ACHJ8C_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO5553249.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(13696..14406)
                     /locus_tag="ACHJ8C_003455"
     CDS             complement(13696..14406)
                     /locus_tag="ACHJ8C_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO5553250.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIAALFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLSHPKNEG"
     gene            complement(14573..15379)
                     /gene="xthA"
                     /locus_tag="ACHJ8C_003456"
     CDS             complement(14573..15379)
                     /gene="xthA"
                     /locus_tag="ACHJ8C_003456"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO5553251.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPISVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
BASE COUNT         4032 a         3833 c         3947 g         3760 t
ORIGIN      
        1 agaggataat tatgattatc ggtatcgacc tgggtacaac taacagtttg gcagctgtct
       61 ggcgaaatgg acaaagtgaa ctgatcccca atgcgttagg taagttttta acacccagcg
      121 tagtttgtgt tgacgaagat ggtatggttt tgacagggga ggcagctcgt gacctgcaac
      181 tcatcaaacc acaaaactgc gcatcaaatt ttaaacgcat gatgggcaca tcaaagacgt
      241 taaaactggg cggacgggaa tttcgtgcgg aagaactctc atcgttgata ttacgtcagc
      301 taaaagaaga tgcagaaaac tatttgtgcg aagaagtcac tgaagcggtg attagcgttc
      361 ccgcatattt tggtgatatg caacgtaaag caacaaaagc cgctgcgaca atggcaggct
      421 taaatgtcga acgtttaatt aacgaaccaa ccgccgctgc actggcgtat ggtttgcata
      481 ataaggatga tgaacatcaa tttctggtat tcgatttagg tggtggtacg tttgatgtct
      541 ccatactgga gttgtttgac aatattatgg aagtacgtgc cagtgccggg gataactttt
      601 taggtggtga agatattgtc gatatcctga tcgatgcata ttgctcacgt cgggatcttc
      661 ctgaaaatat tgaatggcgt gaaccgacac tccagcggca tttacgcatc gaggctgagc
      721 gtgtcaaacg cgtgctgagt gtgcgagatg aagccacttt cagtgtggag attgaaggaa
      781 ggcgatatta ctggcatctg actacagaaa aatttgaatt tttattacag acctttttcg
      841 aacgaatcca tatgccactg gagcgggcaa ttcgcgatgc caaaattaat attagccaat
      901 tagaccaggt tgttcttgtg ggtggcacta cacgtatgcc actgattcgc aaacttgtga
      961 cacgattatt tggtcgtata ccggcaatgc atttaaaccc ggatgaagtc attgcccagg
     1021 gcgcggctat tcaggcagca cttaaagcgc ggcacagcga attacgagat gttgtagtga
     1081 ctgatgtttg cccttatacg cttggcgttg atacctctaa atcgctgggg catacccgcg
     1141 aatcaggcta ttttgcaccc gttattgagc gtaacaggag tattccttgt agtcgcgttc
     1201 gtgcctttta cacggcacat gatcaacaaa cagaggttaa ttttaaaatc taccaaggtg
     1261 aaagcaggat ggtggctgat aatattttcc ttgcggagtt gtcagtgaat gttccgccaa
     1321 aaccggccgg tgatgtgcgt attgatgttc gttttacgta cgacattaac ggtattcttg
     1381 atgtcgatat cagcgtgcca ctgacaggtg ctaaaaatag tctggttatt gaacagaatc
     1441 caggggcatt aacggcagaa caaatacagc agtcgttgtc caaattaagc ctgttaaaaa
     1501 ttcacccacg cgatgaacag ataaaccagg cctttattgc ccgtcttgat aatctctatc
     1561 aactcaccct ggcggaaacg cgagactgga taagcaattg ctcccgtcat ttcagttatc
     1621 tactcgagaa gcaggatcct gatcaacttg ctgattttcg gactaaagtt gaacctacat
     1681 tagactctct ggaaagagaa cgtctgcaat gaactgctgg gaaatattag atttacaacc
     1741 aggtgcggaa gaacgagata ttaagcgtcg ttatgcgcaa ctggtaaaaa attgccgccc
     1801 ggaagatgat cctgcagcct ggcaacaatt gcacgatgct tatgaacagg ctttacgtta
     1861 tgacgacgaa gatcagtggc aagaagagga agaagagcag ccaatcaata tgcccagggg
     1921 tattgtttat cctcgtcctg tgatgacatt tcgcgcaccc aacacctttt ctggccatcc
     1981 acttcctgat gtcgctgtta aaccagaggg ggaattcacc tggcaagccg tgctggatat
     2041 gctctgtgag gagaatgaag ctacccccga acacgccccc atgcaacttt ctaaggcgtt
     2101 atctttcctt gagaaaaaag atctcagctc acgaatgtgc tttgaagaag cgttattact
     2161 gtatcttcat cgggtatttc ggccattatt aactctcgct gctgcacaaa cttttagatg
     2221 gcatttagtt gcaggcagtc gtcaacccgt aatccagaca gaaattaacg aaagttgtct
     2281 tctttattat cgcatcgaaa aagaaatagc cagttatttt tacagtacga tgtcaccttc
     2341 tgaagagtta gaaaatggca aggcgctgtt gtcttcttat cagcaactac acgaagatga
     2401 cagaagtcat aaatggttca aagtggcttt attaaacctc atcaataaat atgatttatc
     2461 tgatgattat ttaagtttaa ttattgagtt aatagggtgg tcatttcaac ctgacccaca
     2521 ccagttctgc tggcaggtat cagaacagtt ttatgtattg tttaatctac ataaaacagg
     2581 atatgaaaaa atacttttcg ccttaaatct tgagcaaggg aaatatcaac aatcaaaaaa
     2641 attaacaagt tataaacgtg ccgcgcagca aggaaattcg gtgtttcaaa atgacttagg
     2701 tgcaatgtat tatattggcg agattatcaa aaaagacttt gtgcaggcaa aatattggtt
     2761 cgagaaatcc gcagggcaag gcaataatga tgcgttatta aatttggcgt taatgtatcg
     2821 tgatggtaaa ggtgtaaata aaaatccaca aaaagctatt tcattatatc taaacgcggc
     2881 taataaaaat catcctctgg cgcagcatag tttggcatgt atgtatcggg atggtgaagg
     2941 tgtggaagtt gacgatgaac aggcctttaa atggtgtcaa aaatcggcag aacaagggta
     3001 cgctgaagca caatatcacc ttgccacaat gtacatagac ggtcggggcg ttgatgtgga
     3061 ttatcaacaa gttgttcact ggctaaattt atcagcagaa cagaaaaata gtaaagcaga
     3121 gtatacgttg ggatatatgt attataaagg aacagagatc cctcaagata tgacaatggc
     3181 aattaatctg ttaaaagctg ccgctgacca tggagatatt gatgcagcct tagttttagg
     3241 tcaactttat gttcatggca gggaaggata cttgcctaaa gatatccacc aggccattgc
     3301 gtatttaaat caagcccagg aaggaggcga ggaacagtcc gccgagatat taggtatgat
     3361 ctattaccag ggatttaaag aaattgcaga aaactctgct ttaagtttta acatgttttt
     3421 taaagcggcg tacgctggaa ttaaaagagc gcaaagaatg ctttctatta tgtatatgga
     3481 aggggatggc acagataaaa attacatttt gtcatgggcg tgggggataa tttctgaagc
     3541 atatcatgat ttggagagca atgactggtt gccagaagtc agagaaagtt tgtcgttgca
     3601 agatttaagc cgggcgcaac ggatggcgag gattctgaaa aaacgttatc aattataaat
     3661 tattttcacg gaacagagag gcacaattca atggcttact ggtgcagcgc agtgtgggca
     3721 aatagaggcg atatttttgt tagctgaaat gtatctgtac ggtacaaaag ataccataaa
     3781 agatgaaaat catgcgctgc actggtacga aaaagcggcg cgtttagggg cgaccgaagc
     3841 gcagcaccag actgcggcaa tgtatgctca ggggacgggc actaaaattg ataataaaca
     3901 agcatggatg tggctaacaa ttgccggaaa taacaacccc tggattgaaa aagatgcgtt
     3961 gcgatgctta atgtctgaac aggatattca ggatgcagaa caaacggctg caaattgcaa
     4021 acgtctgcta aggatttaat taactcaggg aaaatcgtct ggttataagg aaaaccagac
     4081 gattgtaata acgattaaat cacgccctgg cttaacatcg catccgcaac cttcacaaaa
     4141 ccggcaatgt tcgcgccctg cacgtagttg gtttgctcac cttcaccacc atgctcaaca
     4201 caggcatggt ggatatccag catgatgtga tgcaaacgtg cgtcaacttt ctcggctttc
     4261 cagcccaggc gcgcagcgtt ttgcgccatt tccaggcccg atgttgctac accgccagca
     4321 ttagccgctt tacccggtgc aaacagtacg cctgcctgct ggaacagttc agtcgcttcg
     4381 atggtggtcg gcatatttgc cccttcggcg acggctttaa cgccattggc gataagctga
     4441 tgcgcggcgt caacatccag ttcgttctgg gtggcgcaag gcagggcgat atcaaccggt
     4501 acagaccacg gctgttggcc ttcgagatag accagaccaa attctttggc gtaatctgcc
     4561 actcgaccat cgcggctggc tttgatttcg ataagacgcg ccagtttttc tttcgtgaat
     4621 ccgctttcat caactacagt gccgctggag tctgacgcag tgatcactcg agcaccaaat
     4681 tccatcgctt tttcgatagc gtactgggcg acgttgccgg agccagaaac ggaaacgcgc
     4741 atcccttcaa aacccatacc gtggcgtttt agcattgctt ctgtgaaata aaccagaccg
     4801 tagccggtag cttccgggcg aataagactg ccgccaaatg aaaggccctt accggtgaag
     4861 acgcaggcgg tattgttgga gagctttttc atcatccccg ccataaagcc gacttcacga
     4921 ccaccaacgc cgatatcacc tgccggaacg tcagtatccg cgcccaggtg gcgatacagt
     4981 tcagtcatca gcgcctggca gaaacgcata acttcgcctt cgcttttccc tttcggatca
     5041 aagtcgctgc cgcctttacc accgcccatc ggcagggttg tcagggcatt tttaaaggtt
     5101 tgctcgaagc cgaggaattt gagaatggaa aggtttactg aagggtggaa gcgcataccg
     5161 cctttgtacg ggccgatggc agagctgaac tgcacacgcc atgcacggtt gacctgtacc
     5221 tggttgcgat cgtcaaccca taccacgcga aactggatca cgcgctccgg ttcaaccaga
     5281 cgctccagta atgacatctg gcgatatttt ggattttgtt caagaaaagg ccagagtgtg
     5341 gtcattactt cacgaacggc ttgcgcgaac tcggtttgat tcgggtcgcg cttttggaca
     5401 tggttgagga atgactccag agaatatgtc tgatccatag atataaaacc cttatatatt
     5461 aatacgattg tgcttatgtt gtttatgtca tgtgcttttg cagttttcga ctataccatc
     5521 aagaatgtga cggtaagcaa gaaaaaaatg acccaggaaa gcaaattaat aacgagagta
     5581 atctcataac caaatgcgat aactcagacg ctaaagtttt gccgcccggc taccgttatc
     5641 tgtagaagag acaataacgt taaggagttc cctgtgagtc gagcattgat cgccgtagta
     5701 ttagcatttc cattaattac actggctaac ccgcattatc atccggacgt ggaggtaaac
     5761 gtaccgccag aagtgtttag ttcaggcggg cagtcagcgc agccctgtac tcagtgctgc
     5821 gtttatcagg atcaaaatta ttctgaaggg gcagtaatta aagccgaagg aatactgctg
     5881 caatgccaac gggatgacaa aaccctaagc actaatccgc tggtctggcg gcgcgtgaag
     5941 ccataaacgc ctctaataat ggaatgtcag caggggccag cggatatcgc agcgcctctt
     6001 caggtgagca ccagaccagc gcctgatgtt catgtgcctg taacgtcccg tggaagtcgg
     6061 gtacgtgcca ggcatgaaga tggataatcc gccccgaaac ttctcgctga tggctggcaa
     6121 catagtcacc aacagttgct tcgatgccag ttcttcgttt aactcacgca ccagcgcctg
     6181 ccgctggctt tcatccggct cgactttacc accggcaaac tcccataatc ccgcctgatc
     6241 gctatgggca gggcgttgcg cgagtaaaat tttgccatca cgttcgatga tggcggcaac
     6301 aacttcaatc attttcataa attacacacg ggcagataag cgaaaaatgc cataatatcc
     6361 gcactgttgc tcaatcagaa aggctttata tttaaggagt gcctgtgcta gaccgccatc
     6421 ttcatccccg gattaaaccg ttgttgcatc agtgcgtgcg ggttcttgat aaaccgggca
     6481 ttacgccaga tggtttaaca ttagtgggat ttgccatcgg cgtgctggca ctgccttttc
     6541 tggcgctggg ctggtatctg gcggcactag tcgccatttt gttgaacagg ctgcttgatg
     6601 gtctggacgg tgcgctggcg cggcgcagag ggcttaccga cgcgggcggc tttctcgata
     6661 tttctctcga ttttcttttt tacgcgctgg tgccttttgg gtttatcctc gccgcaccgg
     6721 agcaaaacgc cctggcaggc ggctggctgc tgtttgcgtt tatcggcacg ggcagcagtt
     6781 ttctcgcttt tgctgcgctg gcggcgaaac atcagattga taatcctggt tatgcgcata
     6841 aatcgtttta ttatctgggc gggttaaccg aaggcagcga gacgattttg ctgtttgttc
     6901 tgagttgcct ctttccggcc tgttttccgt ggtttgcgtg ggtatttggt gcgttgtgct
     6961 ggatgacgac ctttacgcgg gtgtggagtg ggtatctgac gctgaagtcg ctccagcgtc
     7021 agtaattgca atgttattta ctgctgtccg ggccgcgttc accggttgct accggatttt
     7081 ttggatcgct gctccattcg taccagccac cgtcgtaaac ggagacgtta ttccagccca
     7141 ttgcgcgtgc gtacataaag gtttctgacg cgcgccagcc agtgccgcag tagaatgaaa
     7201 cttgctgatc tggtttgata ttccatgctt tccacatagc ggttatatca tcggcgctgc
     7261 gcatggtgcc atccgggtta tggaaatctt ccatatgcgt cgagtcgcta cctgcgtgtc
     7321 cccaacgtgc tccggctatt tcaccttttg gtttaatata gctgtaaccg ctggtcgtac
     7381 cgataaattc tggccacgaa cgaatgctca ccagcgatgc atcctggcga tgcagcagtc
     7441 cacgcgcttg ttccatatca agcatcagtt gcggttgtgc cgggatcttc acgccgaaat
     7501 ccggttccgc tttcactttc ggtggcgttc cgcgctcaac gggcagaccc gcgtcggacc
     7561 aggtttgcca gcctccgtcc agcaggcgca catctttcac gccggcataa agcataattt
     7621 gcgccacacg cgctgcagcg tatacgtcac gcccgtacag aatgaccgtg gtgtcatggc
     7681 gaatgccgtg ttttgccagc atcgctttca gttgttcatc agaaactttg ttccacagcg
     7741 gttcgctttc cacttcgttg gtgtcgatgt agtcagcgcc gggaatatgg ctgataaggt
     7801 aaaattttgg agcgccccag gccgcttcaa tgactttcca gtcaccggca ggtttcgccg
     7861 taacctcttt accttgttgc aggtcatgca gccactgcgg atataccagc tgttcaaagt
     7921 gcggcagttt ttgcagacgg gaaggttcgc ttagcgcgtc actcaggatg gagatatgcg
     7981 taaaacctgc tttttgcagt cgcgttttga cggcgtcgac atctttgtca ttaccgtaca
     8041 gcgccaccgg ggcatcggct ttcaggttat gttgcttgat ccactcgttg agctgttcgg
     8101 tgctcatttt gtcgagccag ctagcagaga ggtttaaggc ggcaggttca tgaccggaag
     8161 ggccatttaa ggtttgtggc cagccgttat aaaacgcgct ggggcgagta tcaatcgctt
     8221 tgccattttg ttgttgaagc tggtcaagtg taagaggctt cgccagttca gcggcccacg
     8281 aagaagcgca agccagccct aaagccattg ccagcgcggt catttgagaa acacgtttca
     8341 tcgaaaatcc cgacattgta gttttgagag cgccattctg aactgacgca tcgggaaaaa
     8401 ctttgcgtta tcgcagtttg ttgtaatttt ctgaccactg cgccatatcc agaacggggc
     8461 tatcaggagg aacatcctga agatcgtgcg tcacctgcac aacagggatc gccatggcgc
     8521 gaatttcgct gaacacccaa tggcgaaaat tatcgcgcag ggccacatca agacggctga
     8581 atggctcatc cagtagcaac gcttttggct gagcgagaag ggcgcgcagc agagcaacgc
     8641 gcgctcgctg accgccagac aaagtggcag gatcctgatg gaacgctccg tcaaggcctg
     8701 aacgctcaag tgcatcattc acggcattac gtcgggcatt ccccttaagt gtcgccggta
     8761 gcgccagcag taaattttgc ccgacgctga actggtcaaa taacagtgca tcctggaaaa
     8821 gaatgccaat ctgacgctgt gcggtgggta gcatgtcaat ccgttgctca ttaagccata
     8881 gctcccctgt acaagaaaac tgtccggcca gcgcaccaat catccacgaa aacagagtgg
     8941 atttcccgca gccggatggt cccattaacg tgacaatgtc acctttatct actgtaaagt
     9001 taacgtttgt cagcaagcgg ctttctggta aacgtagcga aacatttttc acgcagagca
     9061 ttagcggagt ccttgtctga cataacctac ccattttgcg actaacgcgg tcagggcaaa
     9121 aataataagc ggtaacagca gttgccataa agcctgggcg gcgagaatac cgttgctgcc
     9181 gccgctgctt aatgccaccg cttcagtggt gagcgtcgga aaacgccccg cgcctagcca
     9241 cagcgtcggc atatactgcg caatactgac tgaaaatccc accgcgaagg caatcagcgc
     9301 agggcgcaac atgagtgggc attttacgta aaagaagatt ttggcccgcg accagcccag
     9361 tgtttgcgca atcaaaatta accgtgaatc aatgcgctgc caggcgggtt gcaggataaa
     9421 cagcatccac ggcattaccc acagcagatg cccccagacc accgccgtcc agcttccatc
     9481 caggttcagc catagcgcca gcgtgtactg gcccgccacc agcggcagag cgggtaataa
     9541 aatgggcaac catagccaca actggcgacg ctgtggcccc cattccagcc acagcagtaa
     9601 caggagcaaa gcgatgaatg ccgccaccag ccccattgtc aggctgttga tgagcgcttc
     9661 actattgatc gtcgactgat ccgcgaggat cgccagcaga accacacaca gcacaccggt
     9721 taagggtaaa aaactcgcca gcgtattgcc tggcaataaa ggcgtggcag gcttacgaac
     9781 gccatctacg cggggaatag tacgtcgcca gccacgccat agcagatagc ccagcaaaac
     9841 gtaggcggcg agtaacagca tcagcagcag gctggcgagt gcacctttcg tttgttgatc
     9901 ggcgtcgccc tgagttaacc actgccagct aattaccgcc agcgtcgggg gattacctgg
     9961 cccaagaata atcgccacat ccacgaccga cagcgaccag gcgacaatcg ccagcatcgc
    10021 cattgccagc gcagacgcga cggagggcaa cagcagccag ttcaggcatt gccagcggct
    10081 gtagcccagc gaatccagca caatgagttg ctgcaacagc cgtttttcgc tcaatactgc
    10141 cgctaagatc cacagcagaa atgcgctttc tttcaccgcc agggtgaggc ccagcccaat
    10201 acccaatttg tccattggcg gagtgaaata cgggaaatag tcataaagca gccctccgtc
    10261 agcaaagagc agaagggcgc tggtggcaaa agccacatgg ggaatggcga gcagccacgg
    10321 cagacgggcg cacatacgct gccatttcgg ccctggccac agcgccacaa tcaccagcag
    10381 ggcaatcaac aacgccccga ccgccgcaat ggttgtcgac accagcgtcg ccagtaatgc
    10441 ctgcggtaac tgtggatcgg caaataacgc ctgccagtgc gtcaacgaca aagcaagcga
    10501 tatcagcgtg agagctgccg ggatcagcgg tgcataaatc accgccatca tcgcccacag
    10561 caaaaagatt aatgcgtacc gtaacggtgt agccattctt gttccagcgc atttacccaa
    10621 cctgcgtgcg gttcagccag taccggcggc agatcctgcg gcattctcga ttgcaatgtt
    10681 tcgcgctgcc cgtcaggcag tttttgcgga tcgagaacag aaggatcgcc ccagacagcg
    10741 ggatctgctt tacgcagttg cgcatcgggt gagagcagga aattggcaac taccttcgcc
    10801 gcagcactgg cattcgcgtt ggcaggaatg gtgacgaaat ggacattgcc aatcatcccc
    10861 tcgcgaaaac caaaactgta actgcttgca ggcaaatcgc cgctggcgat tttttgctgc
    10921 gcatgtgcag ggttaaaggt cagcgacagg cgcaatgtgt tagctttcag cagagcatcc
    10981 atccgcgcgg gcgaaggcgg gaaatccttt ccttcgcgcc acaaatacgg atgcagtgca
    11041 tcaagatatt gccacaaggg cgcagtgacg cgggcgaaag tcgcatcgtc cggcgcttct
    11101 tttaatgctg tgggatctgg cgttagcata atcagcaact gttcaagaaa cgccgtgccg
    11161 gtaaagtccg gtgggcgcgg ataggtaacc gtgccgggat tggctttagc aaactccagt
    11221 aaggcttgcg gcgtttgtgg tggctgtggc gtaacatcgc ggcgggcgat aaacgtcagt
    11281 tgtgcgccgc cccagggcga ttcagcccct tctgtaggca ctgaaaaatc ttcccgcacc
    11341 ggaagctgtg tgtcgacata gcgccagttg ggcagagtct ccgcccagcc cgtttgtaat
    11401 aaattggcct cttttaaggt gcggaagttt tcgccgttca cccagagcag atccaccgaa
    11461 ccgcctgttt tgcgtccggc agtagcttcg gtctgaatgc gcttcaccgc gtctgcggca
    11521 tccgcgagac gaacaatctt caggtttata gcgtaatggg ttttcatctc gccgctaacc
    11581 cagtcgagat agcggttaat tgcggtatcg ccgccccagg cgttaaacca gacggtttgt
    11641 cctttggcct cattttttat ttcctgccag tcactggcat gtgttgccaa agaaaacagc
    11701 gataacaatc ccagcaacca cccacaatgg cgcatcttct ctccttaaga acgtttttca
    11761 ggaatacggc acagcccctg agcgggaatg tccatggcag aattaaaacg ggctgacaga
    11821 ttttgcaggc ctatcaatgc cgtcagttcg gtgagcgcct gagcgtcaaa atgcgtagcc
    11881 agtcgggcgc gcagggcatc atcgaccgtt ggcggcgtta cgcttgcggc ttcggcgtac
    11941 tccagcgcca gccgttcttc atcgctaaag agcgggcttt gccgccagtc agccactgcc
    12001 agcagtttat cggtgctgcc ggtacgctcg gcgactttca tactggtgat atccacgcaa
    12061 aactcacaca ggcacatttg cgcaatgcgc gcgctgacaa gcgatcgtac taccggatcg
    12121 agcggtgagc gtttgcgctc cagccagccg acaaacatcg acaccagata aaagataaac
    12181 gggatccgcc cccaccagcg aatcgggctt agcacctcgc cgtaatggcg acgatgcagc
    12241 cacgcctgcg gacgtaaaat gaaaggaatt ttgctaagcg gcggtaaacc catcaggcat
    12301 tcctcttctg gcgctcaagc catattttct tcgccataaa aatcaccacg gtcagggcaa
    12361 acagaataaa cagtccggtg acaaaccaga aagtaccgcc tgttaacatg ctgcccgccc
    12421 aggaataaac aatagtcgcc ggtaattgac caagcccggt ggcgataaaa aacgagcgaa
    12481 aacgtattga agtcaaaccg gcagcatagc tgattggatc gaaagggaca aaaggcaata
    12541 accgacatac caggatggtg tgtttgccgt aacgagtgaa aaagccgtcc atactgtcaa
    12601 gtacggtttt gccggttaat ttttccacca cttcgcggcc catcactctg gcgataaaaa
    12661 agcacagtgc cgcgccggcc atcgaactgg tccacgacag taagccgccc cagaacgcac
    12721 caaacagcga cgcattggca aaggtgatca aaaacgcagg tagcggtgca gcaatggcct
    12781 gtaaaatcat caacaagaac gagacaactg ccgctagtgc gccgtaagac tgaataaagc
    12841 gttctatacc ctgttggtcc actgcggcaa acgccgcaac gctgcgattg ataaactcat
    12901 ggacgctggg aagcagcgcc catgccagca gcacagcgaa caacaggatg atgagggtta
    12961 tacggtagta ccagatttta cgcgattgca tcttcatcat ccgttatcct gctttattcg
    13021 gtgattttaa aggtaaccgt tgccagcgtg ttatccgccg ggagaactga ggcattgcct
    13081 ttgaatttca cttcgccgcg tttttccacc gcaccataag tgtatgttgc attgctgacg
    13141 atgccgaccg ggcagctatc cagacacacc aggcaacccg ttttcttctc ttctgctgcc
    13201 gttaaattac cgccaaagcg catgtccagt ttcttgccat tactgtcaac aatcacttca
    13261 tcgaaggaat acgcttttgc cgccccttgc cagttgaccg aaatatccag tttgctgcct
    13321 gtgacatgag tcgtttcttt attatccatc gtcatgtttt cgcccggcgt accacctgcc
    13381 tctttcaggg cttcataaaa tgctttcggg gtcgcataac ccataaacag cgatttatgt
    13441 ccgttggagc catctttaaa cacaataccg tgacgagtgt catcggtgag gaaacgacca
    13501 ttaatttgca ccagcatggt taccgtatgg ttattggcat cgacacgcag cggttggctg
    13561 ttcgaaagtc cgtcgtattc cacttttgca gccgcattct ctttttgatc gcagccgcta
    13621 agcccagcaa cagccagtaa gcagagtgca gccagtcctt ttttccatga cactgaataa
    13681 tgttgcaaca tcgttctatc cttcattttt tgggtgagaa agtgggctgc ggcgagaagc
    13741 agacagatcc acatgtttgt gtcgggcgta gagttttgcg agctggacga gaataaacag
    13801 tgccaggccc gccagacaga gttgtaaaat aaagcgcagc gtaatgcctt cattggcgag
    13861 atcgcttgcc atcacggtat aaataacaat accgggtagg gtcgtgagtg ccgaaataag
    13921 ggtataaggc cagaaggcga ttgtggttaa tccgtaagcg taattttgaa tattgtaagg
    13981 aaacaacggg attaagcggg tcagaataag aaaatcaata ccgttacgcg caatgccttt
    14041 ttcaatggcc tggaaggtat ggctatggcc aacgtatttc agcagtaaat cacgtcccag
    14101 ccagcgcgcc agcaggaatg agcacgagga ggccagcgtg gcggcaatta atgatagtag
    14161 tgtccctaag agcgggccaa aaactattcc tcccgcgatc accagtatgc ttcctggtaa
    14221 cagaaagagg gcggcaatga tgaataacag aatatagagg ctatagccga acaatccgct
    14281 ctggcgaatg agtgtctgta agtggggtaa atcggtgagc agatcgaata aaccgaaagc
    14341 gtggatagcg taaatgacca gcgcaaaaat aagacaggca aaaagaaatt tacgctcagc
    14401 gttcatcgtg gagtttccta tatcaaactt gataatagag aagtggtcag acaactaaaa
    14461 acgcagtgac ggtataacaa aggacggcag gcaacaaatc attactgaca gtttgttgat
    14521 ttttctcaag gttaattctc ctgacccagt ttgagccagg agatcagcta aattagcggc
    14581 ggaaggtcgc ccagacgggg gcatgatcgg acggtttttc catgctgcga atttcatagt
    14641 cgatgccggt ttctacgcaa cattctgcca gcggttggct ggcgagcagc aggtcgatgc
    14701 gcagaccgcg attatcgtca aaaccttttg agcggtaatc aaaccatgag aaacgatctg
    14761 ctgtttgcgg attcgcatgg cggaaggtat cgaccaaccc ccagctcatc agcctctcca
    14821 tccattcgcg ttcctccgga aggaaagaac attttccggt acgcagccag cgcttacggt
    14881 tctcttcgcc aatgccaata tccaggtcgc caggactaat attcatatcg cccataatca
    14941 gcaccggatt ttcacgcttg agttcggttt ccaggtagtt ttgcagattc tgataaaact
    15001 gcgccttcgc agggaatttt atcggatggt cgcggctttc accctgcggg aagtaaccat
    15061 tgatcacggt gacattgccc agcggcgagg ggatttccgc cataataatc cgccgttgcg
    15121 cctcttcgtc gtcaccagga aagccgcgac gcacggaaat cggcgtctct ttggtcagca
    15181 gcgccacgcc ataatggcct ttctgcccgt gataaaacac gttgtagccg agcttcgcca
    15241 cctcttcgag cggaaacata tcgtcatgaa cttttgtctc ctgcaggcca atcacatccg
    15301 gttggtgctt ttcgacgatg gcttcaagct ggtgaggtct ggcgcgcagg ccgttgatat
    15361 taaaagagac aaatttcata gtcgctgcca tttattcaga taaagagtgc gtggatggta
    15421 gcagaatttc gctttgctta ccgcctgttg ttaagtgatt tgatcgatgg ttgttaatga
    15481 tgtcaacgat ggcgcaaaaa atgcccgctt ttggtgcgcg ttgcgtcaga atggcgcagt
    15541 aatttccagt aaatttcgtc aatgatcaca at
//
LOCUS       DAZFJL010000111        15430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFJL010000111 DAZFJL010000000
VERSION     DAZFJL010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.111"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            45..1103
                     /locus_tag="ACHJ8C_003457"
     CDS             45..1103
                     /locus_tag="ACHJ8C_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO5553252.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKVELYGKMVGERIL
                     TDRESGEKGDNSQDTSYARVGVKGETQINPELTGYGQFELDLEASNRHNPDQTRLAYA
                     GLSYKDFGSFDYGRNVGVAYDAEAFTDMFVEWGGDSWAGTDLFMTNRTNGVATYRNTD
                     FFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTIDGFSFAGAYANSDRTDWQS
                     GDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGDVVNKTQNFEAVLQYQFDF
                     GLRPSIGYSYSKALDVAGYKDSDRLNYIEIGTWYYFNKNMNVYTAYQINLLDKSDYVL
                     AHGLNTDDQLAVGIVYQF"
     gene            1699..2550
                     /gene="hchA"
                     /locus_tag="ACHJ8C_003458"
     CDS             1699..2550
                     /gene="hchA"
                     /locus_tag="ACHJ8C_003458"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO5553253.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRRSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(2658..4016)
                     /gene="hprS"
                     /locus_tag="ACHJ8C_003459"
     CDS             complement(2658..4016)
                     /gene="hprS"
                     /locus_tag="ACHJ8C_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO5553254.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDNREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDALSLNKEVENLLDYLEYLSDEKDIRF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(4016..4687)
                     /gene="hprR"
                     /locus_tag="ACHJ8C_003460"
     CDS             complement(4016..4687)
                     /gene="hprR"
                     /locus_tag="ACHJ8C_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO5553255.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHTLNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4820..5233
                     /gene="hiuH"
                     /locus_tag="ACHJ8C_003461"
     CDS             4820..5233
                     /gene="hiuH"
                     /locus_tag="ACHJ8C_003461"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO5553256.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            5342..6346
                     /gene="msrP"
                     /locus_tag="ACHJ8C_003462"
     CDS             5342..6346
                     /gene="msrP"
                     /locus_tag="ACHJ8C_003462"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO5553257.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAAFSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            6347..6982
                     /gene="msrQ"
                     /locus_tag="ACHJ8C_003463"
     CDS             6347..6982
                     /gene="msrQ"
                     /locus_tag="ACHJ8C_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO5553258.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGLTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            7239..7889
                     /gene="zinT"
                     /locus_tag="ACHJ8C_003464"
     CDS             7239..7889
                     /gene="zinT"
                     /locus_tag="ACHJ8C_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO5553259.1"
                     /translation="MAIRLHKLTVALGVFIVSAPAFSHGHHSHGNPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYV
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(8559..8648)
                     /locus_tag="ACHJ8C_003465"
     tRNA            complement(8559..8648)
                     /locus_tag="ACHJ8C_003465"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8612..8614),aa:Ser,seq:cga)
     gene            8742..9539
                     /gene="mtfA"
                     /locus_tag="ACHJ8C_003466"
     CDS             8742..9539
                     /gene="mtfA"
                     /locus_tag="ACHJ8C_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO5553260.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDVGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFGQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            9640..9715
                     /locus_tag="ACHJ8C_003467"
     tRNA            9640..9715
                     /locus_tag="ACHJ8C_003467"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9673..9675,aa:Asn,seq:gtt)
     gene            10026..10905
                     /locus_tag="ACHJ8C_003468"
                     /pseudo
     CDS             10026..10905
                     /locus_tag="ACHJ8C_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="invasin domain 3-containing protein"
     gene            complement(11075..12127)
                     /locus_tag="ACHJ8C_003469"
     CDS             complement(11075..12127)
                     /locus_tag="ACHJ8C_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO5553261.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNVLKLEQTKLTLVTMTMQDHAILKEIKDLAK
                     NITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCNNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRSIYELPLSDDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDMPIVIVYGPKGEDKARALVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVIVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            12442..13758
                     /gene="shiA"
                     /locus_tag="ACHJ8C_003470"
     CDS             12442..13758
                     /gene="shiA"
                     /locus_tag="ACHJ8C_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO5553262.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISIMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            13860..15314
                     /gene="amn"
                     /locus_tag="ACHJ8C_003471"
     CDS             13860..15314
                     /gene="amn"
                     /locus_tag="ACHJ8C_003471"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO5553263.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         4168 a         3549 c         3352 g         4361 t
ORIGIN      
        1 ttattgatat attcatgaag attataatca caagggaata cataatgaaa agaaaagttc
       61 tggcaatgct ggtcccggcg ttattagttg ctggcgcagc aaatgcggct gaaatttata
      121 ataaaaatgg caataaagtt gagctatacg gcaagatggt tggtgagcgc attctgaccg
      181 accgtgaaag tggtgaaaaa ggcgataatt ctcaagatac ctcttatgcc cgtgtaggcg
      241 tgaaaggtga aactcagatc aaccctgaac tgactggtta cggtcagttt gaactagacc
      301 tggaagcatc aaacagacac aaccctgacc aaactcgttt ggcatatgct ggtttgagtt
      361 acaaagattt tggttcattc gactacggtc gtaatgtcgg tgtcgcgtat gatgcagaag
      421 catttactga tatgtttgtt gagtggggcg gtgactcatg ggctggtact gacctgttca
      481 tgaccaaccg taccaacggt gttgcaacct atcgtaacac agatttcttt ggcatggttg
      541 aaggtctaaa ttttgctctg caatatcagg gtaaaaatga aggtacaggc aactacaaag
      601 caaatggcga tggccatggt ttatctgcaa cttacactat tgacggtttc tcatttgctg
      661 gtgcttatgc aaactctgac cgtactgatt ggcagtctgg tgatggtaaa ggcgaaagag
      721 ccgaagtttg ggcgttgtca acaaaatatg acgctaacaa tgtctatgct gctgtaatgt
      781 acggtgaatc tcacaacatg aattccgacg acggtgatgt tgtgaacaaa acacaaaact
      841 tcgaagctgt tctgcaatat cagttcgact ttggtctgcg tccatccatc ggttactcct
      901 attctaaagc actggacgta gcaggttaca aagattctga tcgtctgaac tatattgaaa
      961 tcggtacctg gtattacttc aataagaata tgaacgtata taccgcttat cagatcaacc
     1021 tgcttgataa gagtgattat gttctcgctc atggactgaa caccgacgac caactggcag
     1081 ttggtatcgt ctaccagttc taatcagcca acgcgccgag gcgtgttact gtaaaacata
     1141 acaccagcct gagttcgtcc ctcatcgaac tcaggctttt tttattggca tttcatcgcc
     1201 atctgcggca cacgcaattg ccaaagcatc tcgatacagc cccttaccct gcccctcctg
     1261 gcacaatatc ctctaccagc aactcttcca gccaacaccc caaatgcatt tctttataaa
     1321 tttaatctgt aacaaaaccg cttcccatat agaaatattt gcaaaaatag tgcatgaaaa
     1381 aagctttttc cctaccagtc cgggatataa atattcatca gcaccacaat attcctcatc
     1441 actaaatctc tccccatcgc ccccaaacct ctgataatgg tctaaaatca ttgaggccac
     1501 ttgcgacgac gttactcaat aaggaaagtt tggcgtgaat aatattcaac accagtgcag
     1561 catcatcatt acatacttat gcccgccaac taccgctttc gatatattga ctcaattttt
     1621 cagcctggtt aaaaatagcg accagctcag tcgcaaatat agtgactacc ctaactaagc
     1681 aacaataagg aatacactat gactgttcaa acaagtaaaa acccgcaggt cgatattgct
     1741 gaagataatg cattcttccc ttcagaatat tcgcttagcc aatatacgag tcccgtctct
     1801 gatcttgatg gcgtggacta tccaaaaccg tatcgcggta aacataaaat tctggtgatc
     1861 gccgcggacg aacgttattt gccgaccgat aacggaaaac tgttctcgac cggtaaccat
     1921 ccgattgaaa cgttgctgcc gttgtatcat ctccatgctg caggtttcga attcgaagtg
     1981 gcgaccattt ccggtctgat gaccaagttt gaatactggg ctatgccaca caaagatgaa
     2041 aaagtaatgc cattctttga acagcataaa tcgttattcc gcaatccgaa gaaactcgcg
     2101 gatgttgtcg ccagcctcaa cgctgacagc gaatatgcag caatctttgt tcctggtggg
     2161 catggcgcac ttattggttt accagaaagc caggacgtgg ctgccgcttt acagtgggca
     2221 atcaaaaatg accgttttgt tatctccctt tgccacggtc cggcggcttt tctggcactt
     2281 cgccgcagcg ataacccact gaatggttat tctatttgcg cattcccaga cgccgcagac
     2341 aaacaaacgc cagatattgg ctatatgccg ggtcatctca cctggtactt cggcgaagag
     2401 ctgaagaaaa tgggcatgaa catcattaat gacgacatca ccgggcgagt acataaggac
     2461 cgtaaacttc tcaccggcga cagtcctttc gcagcgaatg cgttgggtaa actggcggcg
     2521 caggaaatgc tggcagctta cgcgagttaa tccctgttac attgtaatgt aagcgcataa
     2581 tcaatcgcag ccacgttaat actcacgacg tggctgcgtt ttttcacggt taacttatgg
     2641 cgtactgaag cccgatgtta atttctttgc ggtaacgtaa ttcgaaacac gttatgctta
     2701 ctgagatagt gatacgtagc acttcctcca tgtaattcgg caatcgcttt gactaaagaa
     2761 aggccaagtc cctgacctac ggaatgacgc gaattatctc cccgccaaaa tctacggaag
     2821 agtttttcag gctcattaat tttcgttcca ggactggcga tatcaatatt aagagaaccg
     2881 ttggcatcaa gaaaactggt tatatgaata cgcgattttt ctggcgaata tcgaatggcg
     2941 ttaacaataa gattcgataa cattcgttgc agtaaaattt tatccgcaaa gatttgttga
     3001 ttgcactcga ccttaaaacg aatatctttc tcgtctgaaa gatattcaag atagtccaaa
     3061 agattttcaa cttccttatt gagagaaagc gcatccagtt tcaccaaaac attgtttttg
     3121 tctgccctgg caagaaacag tatattctct gttaaccgcg aaatattttc cagctcttca
     3181 atgtttccgg caattgtttt ttgatattca gcgatacttc tggtttgact gagcgtaacc
     3241 tgattctgac ccagtaatgc attaattggc gttctaagtt catgagcgag atcgtcagca
     3301 aattgactaa ggcgctcaaa atctttgact aaagcatgat gcattttatt caacgcctgc
     3361 ccaagaggtt ttagttctct cggtaatgcg ctaacctcaa caggctcccg gttatcgtta
     3421 taattcagcg cttccgttac gccactcaac tttttgatct ctcgcaaccc cgttctgatt
     3481 aacaacggac ttaatactga gcacaatata atggcgacaa tgcaaattat aatgctatta
     3541 attttatact gttcaagcat attatgtctg gctgaagcca atttagccac agtaaccgtt
     3601 aatgatggcg aaacttcatc aatgttaatt cgtaaagcat ctatctctgt atcattaata
     3661 atgcttctgt aaatgcctgc tgcgctgatt gtctcactag caggtatgtt atttaacatg
     3721 tcatcactga catttgtccg gttaacaatt ttattgatgc catcaccatg aatgatcaaa
     3781 atatcctgac taacatccat catccggtta aagtacacag gtaacgtatc tggatttacc
     3841 ccatcaatta acaactgttt gatctgcgct gtccggttaa tgagtgttgt atcatcgcgc
     3901 catttcaact cacttgccag gccattatag agagtccata caattccagc gccagcaaca
     3961 gacagcagca atataaaaag caaggttaaa cggacggtta tagataatct tttcattatt
     4021 tttttaccgc tacgaatgaa tagcccatcc cccggattgt ggcgattagc ttttcaggaa
     4081 aaggatcatc aacttttgcg cggagcctgc gaatggcgac atccaccgta ttggtatcac
     4141 tatcaaagtt gattccccaa atttcactcg caataaccgt tctgggtata atctcgccag
     4201 ctctggaggc cagtagccaa agtaactgaa actccttgcg cgtcagtgta atactgatat
     4261 tgtccctgct cacactttga ctgacagagt ccatttttaa gccactgatt tctaatgttg
     4321 aattcagagt gtgatgttgc cttaattgtg cccgaaccct tgccagcaac tcagaaaatg
     4381 aaaaaggttt taccagataa tcatttgccc cactgtccag ccctctgact ctgtcatcga
     4441 cagaatccct tgcagtaagg caaataacag gggtttgctt tgccgttctc aacgtttgta
     4501 agatctgcca gccatccata cccggaagca taatatccag aatgatcaat gcataatcat
     4561 ccttcagcgc aagataaagc ccatctctgc catcagaaac ggcatcaatg acataacccg
     4621 cttcggaaag cccctgcgtt acccattcct gggtcctttg attatcttca ataagtagaa
     4681 tcttcatgaa aataatatgc cataaatcat atgttataga aacaacctgg ttcattacaa
     4741 aattgtaatg ctgctgtaag gttaccctgg acgttttttc gctatcctca aaactcattc
     4801 acatgacaag gatataaaca tgttaaagcg ttatttagta ctctcggtag caacggcagc
     4861 attttcatta ccttctctgg tttatgccgc acaacaaaac attcttagcg tgcacatttt
     4921 gaaccagcaa acaggaaaac ccgctgccga cgtgacagtc actcttgaaa agaaagcgga
     4981 taacggctgg ttacaactta ataccgccaa aacagataag gatggacgaa ttaaggcact
     5041 gtggcccgag caaactgcga ctactggcga ttaccgtgtc gtatttaaaa ccggggacta
     5101 tttcaagaaa caaaatcttg aaagtttctt ccctgagatt cccgttgaat ttcatattaa
     5161 taaagtgaac gagcattatc atgtgccttt gttactcagc caatatgggt attcaaccta
     5221 tcgtggcagt taatgcgtaa atatgtaaaa gctgatctct gctactgtta gggaggtgac
     5281 tccctgacag attaacagta aacggctctt gttggctaac gacaaaaaag tgtgatggct
     5341 tatgaaaaag aatcaatttt taaaagaatc agatgttacg gccgagtcgg tattctttat
     5401 gaagcgtcgg caggtgttaa aagcactggg catcagcgca gctgcatttt ctttgcctca
     5461 cgctgcgcat gccgatctgc ttagctggtt taaagggaac gatcgtccgc ccgcccccgc
     5521 cggaaaaccg ctggagttca gcaagcctgc cgcctggcaa aataacctgc cactgacgcc
     5581 agcagataaa gtctccggtt ataacaactt ctatgaattc gggctggata aagccgatcc
     5641 cgcggctaat gctggtagcc tgaaaaccga tccatggaca ctgaaaatca gcggcgaagt
     5701 ggcaaaacca ttgaccctcg atcatgatga tttaacccgt cgcttcccgc tggaagagcg
     5761 tatttatcgt atgcgctgcg tggaagcatg gtcgatggtg gtgccgtgga ttggttttcc
     5821 gctgcacaaa ttgctagcgc ttgccgaacc caccagcaat gcgaagtatg tcgctttcga
     5881 aacaatttat gcaccggaac agatgccagg ccagcaggac cgctttatcg gcggcgggct
     5941 gaaatatcct tatgtcgaag gattgcgtct cgacgaagca atgcatccgc tcacactgat
     6001 gaccgtaggt gtttatggca aggcgttacc gccacaaaat ggcgcgccgg tgcgactgat
     6061 tgtgccgtgg aaatatggct ttaaagggat taaatcgatc gtcagtatta agctgacccg
     6121 cgagcgtccg ccaaccacct ggaatctggc agcgcctgac gaatacggtt tttacgccaa
     6181 cgttaatccg catgttgatc acccgcgctg gtcacaggct accgaacgat ttattggttc
     6241 aggcggcatt ctcgatgttc agcgccagcc aacactactg tttaatggtt acgccgacca
     6301 agtggcatcg ctgtatcgtg gcctggattt gcgggagaat ttctaaatgc gtctgacagc
     6361 aaaacaggtg acatggctga aagtttgcct acatcttgcc ggattgttgc cgtttctctg
     6421 gctggtctgg gcgatcaatc acggtggact aggtgccgat ccggtgaaag atattcagca
     6481 ttttaccggt ctcactgcac tgaaattttt gcttgcggcc ttgttaatca cacctctggc
     6541 acgctacgca aaacagccgt tattgatacg cactcgccgc ctgttaggct tatggtgctt
     6601 tgcctgggcg acactgcatt taaccagtta cgcattgctg gagttgggcg tgaataatct
     6661 ggcgttatta ggtaaggagt taattacccg gccttattta acgttaggaa ttatcagctg
     6721 ggttattttg cttgctttag cgttcacttc aacccagtcg atgcagcgaa aactcggcaa
     6781 acactggcaa cagttgcata acttcgtcta tcttgtcgcg atcctggccc caatacatta
     6841 tctgtggtca gtgaagatta tttcaccgca gccactcatc tacgctgggc tggctgtact
     6901 gcttttagcc ttacggtata agaagttgct ttcccttttc aaccggttac gcaagcaggc
     6961 ccacaacaaa ttatctctat aacttcatat tgccgacaaa cttcgcgtta tcccggccgg
     7021 atgcggcgcg agcgccctat ccggcacaca cagatcgcat caagttatgt ttcgtttgtc
     7081 atcaaccttt catcaccaga ataatttatt ttatttcaat aagatagttc aatacgcctg
     7141 agaataccat gctctcattt cctaatgagt tgttgcattt ttcatatgtt acaatataac
     7201 attacacatc atgtacatta actctggagg aaattgtttt ggcgattcgt cttcataaac
     7261 tgactgttgc tttaggtgtc tttattgtta gcgctcctgc cttttcgcat ggtcatcatt
     7321 ctcatggcaa ccccttaaca gaggtcgaac aaaaagctgc taatggtgtt tttgatgata
     7381 ccaatgtaca aaaccgaacg ctcagtgact gggatggagt ctggcaatcc gtttatcctt
     7441 tactgcaaag tggcaaactt gaccccgtct ttcagaagaa agcggatgca gataaaacca
     7501 aaacatttgc tgaaattaaa gattattatc gcaaaggtta tgtgacagat atcgagatga
     7561 ttggcattga ggacggcatt gtcgaattcc atagaaataa tgaaacaaca tcctgtaaat
     7621 atgattacga tggatacaaa atactcacct ataaatcagg caagaaaggc gttcgttact
     7681 tattcgaatg taaagatcct gaaagtaaag cccctaaata tatacaattt agcgatcata
     7741 taattgcacc acgaaaatca tctcattttc acatttttat gggtaatgat tcccaacagt
     7801 ctttattgaa tgaaatggaa aactggccaa cgtattatcc ctatcagttg agtagcgaag
     7861 aagtggtcga ggagatgatg tctcattgat ttttttgcgc tgacttcagt tatttaacag
     7921 aacagtgcct agtagtaaca actataattt accaagacaa ataagtaaga aaaactcaga
     7981 cacgctcata cttgagtcgt gtctttttta ttatgtctgt agaaaaaaac tgagaaaaag
     8041 gcgaacaata aatacacaac tcagccatac agactggcag cgcaaatgtt tccaggagca
     8101 agtcatctgt catggtaaag ctaaccagaa atataggtta attttggtgt ttaaaccttt
     8161 tttacttgtt agtatgatac aaaggctttc aaaaaagctg ggaaattcaa caactcttgg
     8221 aaattatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt ttggctcaat
     8281 tattgacccc ttctctacgc aacttcagtt cccgccagca actttgcggc agtttcatag
     8341 gatcaatgtc taaaagaata atggtgaccg ataagaaaac gactgaataa ctgtagattt
     8401 tcgctcgaaa ccttcctgtc agacccatag cgaatcaagt gctgaatgtc acagtatcga
     8461 acagaaaaca gtgacgatct aacccttcaa gaatatccta cgattgttct gtttaggaaa
     8521 agcaaggcgg gaagtcagga gataagtcat tgataaagtg gcggagagag ggggatttga
     8581 acccccggtg gagttgcccc cactccggtt ttcgagaccg gtccgttcag ccgctccggc
     8641 atctctccgt tcagatggtt gccatgatgc caggaaattt ggcattttaa cagtccctgt
     8701 ccgtgcaatt ttgttcaagt gacgagtttg cgagcaaaac gatgattaag tggccctgga
     8761 aagtacaaga atcagcacat caaactgccc ttccctggca ggaagcacta tcgatccccc
     8821 ttttaacgtg tctgacagaa caggaacaaa gcaaattagt cgctcttgcc gaacgttttt
     8881 tacaacaaaa acggcttgtt cctttacagg gctttgagct gaattcatta agaagctgcc
     8941 ggatagcact tctattttgc ctgcccgttc tggagttagg actggaatgg ctggatggtt
     9001 ttcatgaagt cttaatttat cctgcgccat ttgtggtcga tgatgaatgg gaagacgatg
     9061 tcggtctggt gcataaccaa cgtattgttc agtcaggtca gagctggcag caagggccta
     9121 tcgttttgaa ctggttggat atacaagatt cttttgatgc ttctggtttt aacctgatta
     9181 ttcatgaagt cgctcataag ctggacaccc gtaacggcga tcgcgccagc ggagttccct
     9241 ttatttcgtt gcgtgaggtt gctggctggg aacacgatct tcatgctgca atgaacaaca
     9301 ttcaggaaga aatcgaatta gttggtgaga atgcggcgag cattgatgct tatgctgcca
     9361 gtgatcctgc tgaatgtttt gccgtacttt ctgaatattt ctttagcgcc ccagaacttt
     9421 ttgctcctcg tttcccttca ttgtggcaac gtttcggtca attttatcaa caagatcctt
     9481 tgcagagact gcatcacgct aatgatacag actcgttttc ggcgacgaat gttcattaat
     9541 taacaacttt gcagattaat taaccaattg aaatggctta tgaaatttag tgttgacaga
     9601 caaggtaccg ctaagtaata tgcgccccgt tctcacgatt cctctgtagt tcagtcggta
     9661 gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc
     9721 taaagattcg cttttttagc gcaatgtcac tgctcttagt tgagcattgc tttttaacgg
     9781 atagcgggtt tttgacatct taagcgccct caatcttcat ggttgagggc gttttgctat
     9841 gaacgccatc accattttcc cctcgattat aaaacttgag taattcagta gtctcccctc
     9901 ttgcaactca cacccaaaac tgcttaacga aaagttatta attttcaatc atattgctat
     9961 caggatctac tttttttcgc tgtgctagaa agggcgcatt tatgttagct cgttcaggga
    10021 aggtaaccgt gaaggttact ggcaactcaa gcaccgccca tgttgctagc tttatcgctg
    10081 atccatcgac tatcgccgcc accaacagtg atttaagtac cttaaaggca acggttgagg
    10141 atggaagtgg taacctgatc gaaggtctca ctgtgtactt cgccttaaaa agcggctcca
    10201 ccacattaac gtcattaaca gcagtgaccg atcaaaacgg aatcgcgaca acaagcgtga
    10261 aaggagcgat aacggggagc gtcactgtaa gcacagtcac aagcaaacag tagatatatc
    10321 gctagtggca ggcccagcag acgcctcgca gtccatcctt aagaacaacc agtcatcact
    10381 gaaaggggac tttaccgata gtgctgagct acatcttgtt ctgcacgata tatcaggcaa
    10441 tccgatcaaa gtttcttaag ggatggaatt tgtgcaatca ggtacaaacg tgccctatat
    10501 gaaaattagc gcaattgatt acagtcaaaa tatcaacggt gattacaaag ctactgttac
    10561 aggaggcgga gagggtatcg caacgctgat ccccgtattg aatggtgttc atcagacggg
    10621 cctgagtacc acgatagaat tcattagcgc ggaaaccaga ccaatgaccg gtacagtgtc
    10681 agtgaatggt gcaaacctac cgacagcttc attcccttcg cagggattta ccggggcgta
    10741 ttatcagttg aataatgaca gctttgcacc aggaaaaacg gcggctgatt atttgtttca
    10801 agctcggcct cctgggtagg cgttgatgct actggtaaag tgacattcaa aaatgacggt
    10861 gatagcaata ctgtaataat cacagcgccc ccaagatccg gtggtgctat ttaccaaaca
    10921 gttccgcatg aatcgcgttc ggtgtgacta tgatcatttt cggtgagtat tgctttgctc
    10981 caaaggctgg ttttctgtga tgattgacca gcctcagagt attgatgaca ctaccgttca
    11041 taacagatcg gcattactat gcataagtac tgcctcaaat tctcgccaaa acacttttta
    11101 atgcctttgc gaattcatcc agacttatat tgtcaatttt ttgcccaacg atgactgact
    11161 ccgaaacatc ggccattgcg ggccatcgcg ttttgtaatc agcataaatt gcaacaacag
    11221 gggtattata ggcacttgcc atatgtaata ttgaggtgtc aggagttatt gcaatataag
    11281 catcttttat gattgctgct gaacgtttaa tcgagggtga taaggataaa cggtagacat
    11341 tattataaca atccactaat gccctggctt tatcttcccc tttgggtcca taaacgatca
    11401 ctattggcat atctgtttca ctttgaattt ttgctattag attttctgca atcgataatg
    11461 aaaatgtacg ttcctgcgag ctaccttcta aattgagcgc aatgtaagat cctaacgatc
    11521 gcatttcctc gcgtacctca tcgagtacat catcgcttaa tggcaattca tatattgacc
    11581 tgactgccgg aaaacccgcc tcacgcatca taaatgccca tgtcatgggt acaggagccc
    11641 ggagattctg atccatccgg gacgcgttat tacacaaagg ggaatagcac ttcatggtta
    11701 aaccgacgac ctggaaattc gtttttgctt tcaactgact gataaatatc atcgttttca
    11761 ggttcttttt acgcatcgcc tctatacaaa gatccggcgt accgtattgc agtgttatgt
    11821 tcttcgctaa atcttttatt tcttttaata ttgcgtgatc ctgcatagtc attgtgacta
    11881 atgttaattt agtctgttca agtttgagca cattaaagac ttctaaatta attgtcgacg
    11941 taacaattaa tagatgctta attttatgca attcaagcgc ccgaataaca ggaaagatgg
    12001 ccatagcatc gccaatctga tcgggaatat gtatgacaac aaagtctgtt ttttcaatat
    12061 taaaattata agctttataa tcgtagtaac taaatgcaat acgtctcaac aatgatacta
    12121 aaaacataac taacctcgcc tccctactgg ttataatgca atgcagtcta tcagaatcat
    12181 cagggtgcaa ttttgtgcat atgcggactt ttatgtttca tatctcgaac ctgtgggtcc
    12241 tctgcttaat ccttaaacaa caccagcaac tcctgcgctt tcatcctcca tcgaattttt
    12301 catgttgccg ctaatcagcc ataaaatcat ttgcagatgc gctctgtcga ggtagtctca
    12361 taaggttcgt ttatagatcg acggcaatgt gagttacctt ttccatacta attataaaaa
    12421 gacagtacaa acaggatcat tatggactcc acgctcatct ccactcgtcc cgatgaaggg
    12481 actctttcgt taagtcgcgc ccgacgagct gcgttaggca gtttcgctgg tgccgtcgtc
    12541 gactggtatg attttctact ctatggcatt accgccgcac tggtgtttaa tcgcgagttt
    12601 ttcccgcaag taagcccggc gatgggaacg ctcgccgcat ttgccacctt tggtgtcggt
    12661 ttccttttcc gaccgctcgg cggtgtcatt ttcggtcact ttggcgaccg actgggacgt
    12721 aagcgcatgt taatgctgac cgtctggatg atgggcatcg cgacagcctt gattggcatt
    12781 cttccttcct tctctaccat tgggtggtgg gctcccattt tactggtgac gctgcgtgcc
    12841 atacagggat ttgccgtcgg cggcgaatgg ggaggcgcag cgttgctttc cgttgaaagt
    12901 gcgccgaaaa ataaaaaagc cttttacagt agcggtgtac aagttggcta cggagtaggt
    12961 ttactgcttt caaccggact ggtttcattg atcagtataa tgacgactga cgaacagttt
    13021 ttaagctggg gctggcgcat tcctttcctg tttagcatcg tactggtact gggagcattg
    13081 tgggtgcgca atggcatgga ggagtccgcg gaatttgaac aacagcaaca taatcaagcg
    13141 gccgcgaaaa aacgcatccc ggttatcgaa gctctgttac gacatcccgg tgctttcctg
    13201 aagattattg cactacgact gtgcgagttg ctgacgatgt acattgttac tgcctttgca
    13261 cttaattatt caacccagaa tatggggtta ccgcgcgaac ttttccttaa tattggtttg
    13321 ctggtaggtg gattaagctg cctgacaatt ccctgttttg cctggcttgc cgatcgtttt
    13381 ggtcggcgca gggtttatat cacaggcgcg ttgatcggaa cgttgagcgc atttcctttc
    13441 tttatggcgc ttgaagcaca atctattttc tggatagttt tcttctccat aatgctggca
    13501 aacattgcgc atgatatggt ggtgtgtgtg caacaaccga tgtttaccga aatgtttggt
    13561 gccagttatc gctacagtgg tgctggagtc ggttatcagg ttgccagtgt ggttggcggt
    13621 ggatttacac cttttattgc cgctgcactc atcacttact ttgccgggaa ctggcatagc
    13681 gtcgccattt atttgctggc tggatgcctg atttctgcaa tgaccgcttt gttgatgaaa
    13741 gacaatcaac gcgcttgata gcctggcgaa gatcatccga tcttcgcctt acacttttgt
    13801 ttcacattcc tgtgacatac tatcggatgt gcggtaattg tatggaacag gagacacaca
    13861 tgaataataa gggctccggt ctgaccccag ctcaggcact ggataaactc gacgcgctgt
    13921 atgagcaatc tgtagttgct ttacgcaacg ccattggcaa atatattaca agtggcgaat
    13981 tacctgatga aaacgcccgc aaacaaggtc tttttgtcta tccatcactg accgtaacct
    14041 gggacggtag cacaactaat ccccccaaaa cgcgcgcatt tggtcgtttt actcacgcag
    14101 gcagttacac caccacgatt actcgcccta ctctctttcg ttcgtatctc aatgaacaac
    14161 ttacgttgct gtatcaggat tatggtgcgc atatctctgt gcaaccctcg cagcatgaaa
    14221 tcccttatcc ttatgtcatc gatggctccg aattgacact tgatcgctca atgagcgctg
    14281 gtttaactcg ctacttcccg acaacagaac tggcgcaaat cggcgatgaa actgcagacg
    14341 gcatttatca tccaacggaa ttctccccgt tatcgcattt tgatgctcgc cgggttgatt
    14401 tttccctcgc acggttgcgc cattataccg gtacgccagt agaacatttt caaccgttcg
    14461 tcttgtttac caactacaca cgttatgtgg atgaattcgt tcgttgggga tgcagccaga
    14521 tcctcgatcc tgatagtccc tacattgccc tttcttgtgc tggcgggaac tggatcactg
    14581 ccgaaaccga agcgccagaa gaagccattt ccgaccttgc atggaaaaaa caccagatgc
    14641 cagcatggca tttaattacc gctgatggtc agggtattac tctggtgaat attggcgtgg
    14701 gaccgtccaa tgctaaaacc atctgcgatc atctggcagt gttacgcccg gatgtctggt
    14761 tgatgattgg ccactgtggc ggattacgtg aaagccaggc cattggagat tatgtccttg
    14821 cacacgctta tttacgcgat gaccacgttc ttgatgcggt tctgccgccc gatattccta
    14881 ttccgagcat tgctgaagtg caacgtgcgc tttatgacgc taccaagtta gtcagcggca
    14941 ggcctggtga ggaagtcaaa cagcggctgc gtacaggtac tgtggtaacc acagatgaca
    15001 ggaactggga gttacgttac tcagcttctg cacttcgttt taacttaagc cgggccgtag
    15061 caattgatat ggaaagtgca accattgccg cgcaaggata tcgtttccgc gtgccatacg
    15121 ggacactact gtgtgtttca gataaaccgt tacatggtga gattaaactt cccggccagg
    15181 ctaaccgttt ttatgaaggc gctatttccg aacacctgca aattggcatt cgggcgatcg
    15241 atttgctgcg cgcagaaggc gaccgactgc attcacgtaa attacgaacc tttaatgagc
    15301 cgccgttccg ataataagtt atgaaccttc ttatcagaaa tagatgtgaa ggattaagta
    15361 agacatctgg caaatagcct gcaataaacg cgggctgttt gcttccttgg gcagatgcga
    15421 gttttattat
//
LOCUS       DAZFJL010000112        15207 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZFJL010000112 DAZFJL010000000
VERSION     DAZFJL010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.112"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(48..1097)
                     /locus_tag="ACHJ8C_003472"
     CDS             complement(48..1097)
                     /locus_tag="ACHJ8C_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO5553264.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPRFNKELT
                     RKLFKLQEMLPFLNLHMSPERILQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGIIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            1196..2446
                     /locus_tag="ACHJ8C_003473"
     CDS             1196..2446
                     /locus_tag="ACHJ8C_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5553265.1"
                     /translation="MKHCCKNVVILMPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFLSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFV
                     GTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFP
                     ALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVA
                     IALLLTWRLKKRPPVEIPEAASSS"
     gene            complement(2450..3007)
                     /gene="dcrB"
                     /locus_tag="ACHJ8C_003474"
     CDS             complement(2450..3007)
                     /gene="dcrB"
                     /locus_tag="ACHJ8C_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO5553266.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADNQQKAQTTAENIINTLVIQ"
     gene            complement(3080..3745)
                     /locus_tag="ACHJ8C_003475"
     CDS             complement(3080..3745)
                     /locus_tag="ACHJ8C_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO5553267.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRHWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            3966..4211
                     /gene="tusA"
                     /locus_tag="ACHJ8C_003476"
     CDS             3966..4211
                     /gene="tusA"
                     /locus_tag="ACHJ8C_003476"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO5553268.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(4472..6670)
                     /gene="zntA"
                     /locus_tag="ACHJ8C_003477"
     CDS             complement(4472..6670)
                     /gene="zntA"
                     /locus_tag="ACHJ8C_003477"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO5553269.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNTNDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQASDEPQESRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGVFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRK"
     gene            complement(6744..7370)
                     /locus_tag="ACHJ8C_003478"
     CDS             complement(6744..7370)
                     /locus_tag="ACHJ8C_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO5553270.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            7511..7870
                     /locus_tag="ACHJ8C_003479"
     CDS             7511..7870
                     /locus_tag="ACHJ8C_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO5553271.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(7873..8142)
                     /locus_tag="ACHJ8C_003480"
     CDS             complement(7873..8142)
                     /locus_tag="ACHJ8C_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO5553272.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(8132..8728)
                     /gene="rsmD"
                     /locus_tag="ACHJ8C_003481"
     CDS             complement(8132..8728)
                     /gene="rsmD"
                     /locus_tag="ACHJ8C_003481"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO5553273.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAISFLAQKGTPNNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     EKGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            8878..10374
                     /gene="ftsY"
                     /locus_tag="ACHJ8C_003482"
     CDS             8878..10374
                     /gene="ftsY"
                     /locus_tag="ACHJ8C_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO5553274.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEHAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELSLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
                     AAEAAEEAVMVVSPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            10377..11045
                     /gene="ftsE"
                     /locus_tag="ACHJ8C_003483"
     CDS             10377..11045
                     /gene="ftsE"
                     /locus_tag="ACHJ8C_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO5553275.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            11038..12096
                     /gene="ftsX"
                     /locus_tag="ACHJ8C_003484"
     CDS             11038..12096
                     /gene="ftsX"
                     /locus_tag="ACHJ8C_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO5553276.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            12341..13195
                     /gene="rpoH"
                     /locus_tag="ACHJ8C_003485"
     CDS             12341..13195
                     /gene="rpoH"
                     /locus_tag="ACHJ8C_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO5553277.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            13467..14570
                     /gene="livJ"
                     /locus_tag="ACHJ8C_003486"
     CDS             13467..14570
                     /gene="livJ"
                     /locus_tag="ACHJ8C_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO5553278.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(14758..15141)
                     /gene="panM"
                     /locus_tag="ACHJ8C_003487"
     CDS             complement(14758..15141)
                     /gene="panM"
                     /locus_tag="ACHJ8C_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO5553279.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPDVSCWWMADAGV
                     EDRGVMTAFMRALGFTAQQGGWEKH"
BASE COUNT         3692 a         3907 c         4150 g         3458 t
ORIGIN      
        1 ggccactgtt agataaacgg tggccattgc ctgctcagga ggtgctatta acccggtaac
       61 cgacttttcg gtctgcctgg ccccagtaaa atcgccagtt tgctgccgcc tttggtggtt
      121 tccatccaga ttttacacac gctggttaat ggcaccgaaa gtagcatccc taccgggccg
      181 agcagccatc cccaaattaa caatgaaaga aataccacca tggtggacat ccccaggcga
      241 tggcccatca tccgtggttc taaaatattg ccgatgacca tatggaccac taaaaacaat
      301 gctccgacca gaatacattc gtaaatacca ttaaacagca gcacctgaat cattggcggt
      361 acggcggaaa ttaccgcacc gatattgggc acgtagttga gcaaaaacgc cagtactgcc
      421 cacatcagcg caaactgcac ccccatcagc tccagcccca gccagacgat tatgcctgtc
      481 cataaactga gtagcgtctt taacgccaga taatgcgaaa cgcctttaag tgcgcggtgt
      541 agtccggcga tgtgaatctg tggattattc aacgcgaaac gcattttgta agggacgtgg
      601 cgcacttcaa acagcataaa aactacggtc atcaccagca aaagcacgct cgccattgcc
      661 ccggaaagcc cggtcattag cgccgtggtg aaggtaacca ctttttccga gtccatccgc
      721 tgtagtattc gctccggcga catatgcaaa ttaagaaaag gcaacatctc ctgcaattta
      781 aaaagtttgc gcgtcagctc cttattaaac ctcggcagca tagagataaa ttcgttaaac
      841 gatgccgcca gtacgccgac cagcgcggtg agtgcgatca gcatcaccac aaccacaatc
      901 gtaatggcaa cggggcgttg taccccccga cgaataaacc aggtgacgag cgggttgaga
      961 acgatggcaa aaaacagcgc cagtaatagc tgcacaatga tatctgccgc tgcgtgaatg
     1021 cccgcgagga tcactaccag cgaggccaac ttgagcagaa tgtgcatgcc cgttttatcg
     1081 ggttgagggg tttccattgg ggcttccttg tgactttttg tattaagtgt agcgggagtc
     1141 acgccagcaa tattctgatt cttactgctg attttcacgt cagcacatgg taaaaatgaa
     1201 acactgttgt aaaaatgtgg tgatcctcat gcccgaaccc gtagccgaac ccgcgctaaa
     1261 cggattgcgc ctgaatttgc gcattgtctc cattgtcatg tttaacttcg ccagctacct
     1321 taccatcggg ttgccgctcg ctgtattacc gggctatgtc catgatgtaa tgggatttag
     1381 tgcattctgg gcagggttgg ttatcagcct gcaatatttt gccaccttgc tgagccgtcc
     1441 tcatgccgga cgttacgccg atttactggg acccaaaaag attgtcgtct tcggtttatg
     1501 cggctgcttt ttgagcggtc tgggatatct gacggcagga ttaaccgcca gtctgcccgt
     1561 catcagcctg ttattactgt gcctggggcg cgtaatcctt gggattgggc aaagttttgc
     1621 cggaacggga tcgaccctgt ggggtgttgg cgtggttggt tcgctacata tcgggcgggt
     1681 gatttcgtgg aacggtattg tcacttacgg ggcgatggcg atgggtgcgc cgttaggtgt
     1741 cgtgttttat cactggggcg gattgcaggc gttagcgtta atcattatgg gcgtggcgct
     1801 ggtggccatt ttgttggcga tcccgcgtcc gacggtaaaa gccagtaaag gcaaaccgct
     1861 gccgtttcgt gcggtgcttg ggcgcgtctg gctgtacggt atggcgctgg cactggcttc
     1921 cgccggattt ggcgttatcg ccacttttat cacgctgttt tatgacgcta aaggttggga
     1981 cggtgcggct ttcgcgctga cgctgtttag ctgtgcgttt gtcggtacgc gtttgttatt
     2041 ccctaacggc attaaccgta tcggcggctt aaacgtggcg atgatttgct ttagcgttga
     2101 gataatcggc ctgctactgg ttggcgtggc gactatgccg tggatggcga aaatcggcgt
     2161 cttactggcg ggggcggggt tttcgctggt gttccctgca ttgggcgtag tggcggtaaa
     2221 agcggttccg cagcaaaatc agggggcggc gctggcaact tacaccgtat ttatggattt
     2281 atcgcttggc gtgaccggac cactggctgg gctggtgatg agttgggcgg gcgttccggt
     2341 gatttatctg gcggcggcgg gactggtcgc aatcgcgtta ttactgacgt ggcgattaaa
     2401 aaaacggccc ccggtggaaa tacctgaggc cgcctcatca tcttaaaact tactgaataa
     2461 ccagcgtatt aatgatgttt tctgcggtgg tctgtgcttt ttgctgattg tcagcgggca
     2521 gcgtaatttg catggttagc agttgattac ccacgttacc cagaataacg gaagagtacg
     2581 ccgtctggcc tttcgcggag ataatgctgt ctaactgctg cattttgtga cctttcagct
     2641 caatggcttt attggttacc acttgcagct gcggatcgcg gctacgttgc tgatcttcca
     2701 gacgcttcgc cagcaccgcc agatcttctt tcggatcatc gcccatgatg acgatcactg
     2761 ctttctgccc ggtggcgtcg gaccagacat gcatgttatt ggcctgcgtt cccagcttac
     2821 cgctctggtc ggtcatatcc gctggcagcg agaaacttaa cttgccatca agcaggttga
     2881 cgggattccc ggtcgcgtta ctttctgcga ccgaaccctg cgccgtagcg ttagtgtctt
     2941 tatcatcaca ggccgcaagc cccataacca gcaggccaat tccgacatat ttaaccagat
     3001 tgcgcattga cttcttcctt tcgataaacg gccataacgg ctcattcatc catcttatca
     3061 caactctgat aacgaacctt taactcgcct gcaaagcgtt gatttcggat ttatctgcca
     3121 gtcttttcaa cagcatattg agtagtacgc catacattgg caggaagaaa acgatactga
     3181 ttaacacttt gaaacagtaa tcgaccagcg cgatttccat ccagtgttca gccataaagg
     3241 catccgggct acgccagaag gcaatgaaga agaaggccag cgtgtcgctg acgttaccga
     3301 acagtgtgga cgctgtcggt gccagccacc agtgacgact ctgacgcagg cggttaaaaa
     3361 cgtgcacgtc gaggatctgc cccagcgcgt aggccatgaa actggcggtg gcgatacggg
     3421 cgacaaacag gttgaagtgg gcgagcgcgc cgaatccttg ccatgacccc atataaaaca
     3481 gcgatgagat gacgtaggag atcagcaatg cggggatcat taccgcaaag atgatgcgac
     3541 gagccagcgg cgcgccaaaa atacgcacgg tcaggtcagt agcgagaaag ataaacggaa
     3601 agctaaacgc gccccaggtg gtatgaaaac caaaaatgga gacgggaagc tgaaccagat
     3661 agttactgga ggtgatcacc agaagatgaa ataacgataa ccagaacaac gccttatagc
     3721 gttgcgtttg cgagaaaacg ttcatattgt acctttttga ttaaccattg gggtgaggga
     3781 acccaatacg tacgacacgt ctgttacctt aacataataa catgctgacc cttttttcga
     3841 gccgccgcat gatactgctt tacgttgaca atgcaatggt taattttcac gcaatcgtta
     3901 acctggtttg cttacggact cacagggcgt aaactagcgc cgttttttta tgtgatgaga
     3961 agaaaatgac cgatctcttt tccagccctg accacacact cgacgcactt ggcctgcgct
     4021 gcccggaacc agtaatgatg gtgcgcaaga cagtgcgcaa tatgcagcca ggtgaaacgt
     4081 tgctgattat tgctgacgat ccggcgacga cccgtgatat tcctgggttt tgtaccttta
     4141 tggaacacga actggttgct aaagagacgg atggactgcc ttatcgttat ttgattcgta
     4201 aaggcggttg atgggggaag atgggcttcg atactgcctt tcccttaccc tctccccaga
     4261 gaggcgaggg gaccgatcgc actcaatgtt gctgcttgaa accggtttgt aggccggata
     4321 aggcgttcaa gctgcatctg gcgatacggt gcacaatgcc tgatgcgacg ctgtcgcgtc
     4381 ttatcatgcc tacgagttcg atgctctgtg taggccgtat aaggtgttca cgccgcatcc
     4441 ggcattgttg gaccgtagac tgatagcctc tttatttcct gcgcaataac cgtaacgcat
     4501 tcgccgttac cagcaccgtc gcacccgtat ctgccagcac cgccagccac aggccggtca
     4561 tccctaacag cgtcgtgacg aggaaaacgc ccttcagccc cagcgcaata gtgatgttct
     4621 ggcggatatt ggcgtgagtg gcgcgtgcca gttcaatcat ttgcaccaga ccgcgcaggt
     4681 ggttatgggt taatgctgca tcggcggttt ccagcgccac gtctgtgccg ctacccattg
     4741 caatcccgat ggcggcagct ttcatcgctg gcgcgtcgtt aataccatca ccgaccatcg
     4801 ccagtggcgc atgttgattc agctcggtca ccgctttaac tttatcttcc ggcaagaggc
     4861 ccgctttaaa ttccagcccc agttccccgg cgattgccgc tgctgcgcgt ggattatcgc
     4921 cggtgaggat cacccctttg acacccagcg cgttcagttc actgatagca gttgcagcat
     4981 cggcgcgcag ggtatcctgc aacgcaatga caccaagcac gtcatcatta cgtactacca
     5041 gcactaccgt ttgcccggcg ctttccagtt cattaatcaa accagcaaat gcatcagcag
     5101 gatgttttcc ggcagcgcat atcaacacgc gctcaccgtt aacctgcgct tcaatgccag
     5161 acccgaccag cgcccgctgt gattgggcgg tgggaatggc gagttcagca acctgtgctt
     5221 cgcgcacaat ggcctgtgcc agtggatgcg tcgcgccttg ctcgaccgcc gccgccagtg
     5281 tcagcagttc agattcacta atacccgttg ccggatgaat cgcggtaacg cgcggtttac
     5341 cgacggtcag cgtaccggtt ttatcaaacg ccacctgggt aacacgaccc agctgctcca
     5401 gcgccgcgcc gcctttaatc aacgccccac gacgcgctgc tgccgccagc ccggaggtaa
     5461 tcgccgcggg cgtggagata actaaagcac acgggcaacc aatcagcagc agcgtcagcc
     5521 ctttataaat ccactcctgc cagctggcgg caaacagcag cggcggcacc agcgttacca
     5581 gcagggcgac ggccataatc gctggcgtgt aaatacggct gaaacggtcg ataaaccgct
     5641 caataggtgc gcggcgctct tcggcttctt caatcagttt cagaatccgg tcaatggcgc
     5701 tggcacccgg ttctgacagc acttccagcg ttaccagacg gtctacgctg gtggcgcctg
     5761 caggaacttt atcgcccgtc gcgcgctcca caggaatgga ttctccggtc agggcacttt
     5821 catcaaaact ggcaaacggt gagagcagtt taccgtcggc aggcaaacgc ccacctgccg
     5881 caacttcaat cacatcgcca ggccgcaggc tgtttattgc cacctcttca cgctcaccgt
     5941 tacgcagacg cgtggcggtt tctggcttca gcgccattaa cgcgctaacg ccctgacgcg
     6001 cgcggctggc ggcccagcct tccagtcgtt caccaatcaa aaacagcagc aacaccatcg
     6061 cagcttcagc cgttgcgcca ataaacagcg caccaatagc ggctacgctc attaaggttt
     6121 caatggcgaa gtagctgcca gatttgatca gccgtaatgc ctgacgagca attgggtaca
     6181 gcccaaccag cgtggtcgcg ataaacgcca gttgaccaaa cggatgattg aactgctcca
     6241 gcccccaact gattgccatc atcacgataa gcgtaatcag cggcagattc tctttcaggc
     6301 gtgattcttg tggttcgtcg gaggcctgtt catcgcgcag ggaatagccc gctttttgca
     6361 ccgcagattc aacttgtgcc cgtatgtcat tgtcggcatc caccaccagt ttttcggtgg
     6421 cgaacaacac ctgtacctga ttcacgcctg caagctggcg cacggcattt tccactttgc
     6481 gcgcacaggc ggcgcagtcc ataccgctga ctttccagct atagcgggtg ccggagacgt
     6541 tttcagagag agttggcgtg ctggaacatg cgccgtcgca gcaacagtcg ttggtgttct
     6601 gtaccgtagt tagcggtttg aacgcagcaa attgaggggc tttcttgccg tgattgtcag
     6661 gagtcgacat ggcatcctcc ggttaagttt tttctcatta accgaaggat acactctgga
     6721 gtcgactcca gagtcaagtt ttatcagaga tacagcgagc ggacgatgag gaagtgacca
     6781 gcaaagtagc aggccgcggc aatggcgtta tccgcacgga agcgtcggcg atagtgacta
     6841 cccagccaga caaagttact gataaacagc aacgaagcgc cgacaaacgc agagagcgcc
     6901 ggagcggtcg gacggaagaa ccacagttca cctgccagcc acaccatcac cagcgtcatg
     6961 ccgataaacg tgcagatagg ccaacgatac tcttccagac gcgtccagat aatcgccagt
     7021 aacagcgcgc ccagaaccag cagcaccaac ggcagaggcc agaagaaaga gagcgtcatc
     7081 tgactggcga aatagatggt gtacagcagg tgcgagagga aaaacgcgcc gatggcgtac
     7141 atcagacgtt gacgtggcaa cagggttagc gcatcgccca gcagtgaggc acacagcccc
     7201 gccagcacca gatagctaat ggcgtcgaac atcggcgctt gccaggccag caacagcagg
     7261 agaagaaggg ttaacggttt aaacacccag cgttgccagg ttggcccacg atacgatgca
     7321 tccacagata gccatgcgga aagacagaca gcgataaacg accaaagcat cttagttcct
     7381 tgaattcgtt attttttcgt aagcggaatg cctacggtct tgtttccagt ttagtggcga
     7441 gggtggacgg atgacaacac cataactggc agggtatgct tatttccgca ttttccgatg
     7501 agggataaag atgagtaaac cacctctttt ctttattgtt atcattggct taatcatcgt
     7561 cgccgcatcg tttcgtttta tgcaacaacg acgggaaaaa gctgataatg atatggcacc
     7621 gctccagcaa aagctggtgg tggtgagcaa caagcgggaa aaaccgatta acgatcgccg
     7681 ttcgcgccag caggaagtga cgccggcagg caccagtatg cgctatgagg caagcttcaa
     7741 accgcaaagc ggaggaatgg agcagacgtt tcgcctcgac gcccagcagt accacgccct
     7801 gacagtgggc gataaaggta cgctgagcta taaaggaacg cgctttgtca gctttgtagg
     7861 cgaacaataa ggttattttt taactttaaa tttcttctgc cagaccagca gttcaaacac
     7921 gccgaaaagg aaaatccgca ccttttcggc ggtggtcatc tgcgggccat ctttcggtaa
     7981 agtggatttc aatagcgcca gctgcatacc atgcatcagc acggtaaaaa tcagcgccac
     8041 gttaacgaaa atattcagcg ggcgtgggaa gggatgcact aggttgagga ttaaaaatcc
     8101 ccaaacacag agcattaaca aacgaccaat attaatcagc atcactttct ccttgtgctt
     8161 cgcgttgata cagccgataa gccacctgac ccgccacttt ttcccgatgt aatgaccagt
     8221 ttgccggaac agtgggcaga cccttttcga cttcgctttc gacataaatc agggcttcgt
     8281 cagccagcca gccgttatct tccagtaaat ttatcgtctc ttctaacaag ccacggcgga
     8341 acggtggatc gacaaacacg atattattcg gtgtgccttt ttgcgccagg aatgaaatcg
     8401 cgttgctgtt caccacgcgt gcattgcctg cttttagtgt cgccagattc ttaattaact
     8461 gctgagaaac cgcacgatcc atctcaatca acgtggcccc cgcagcgtag cgcgataacg
     8521 cttccagccc cagcgcgccg ctcccggcga agcaatccag acattgggcg tcaacaatga
     8581 ccggagccag ccagttaaac aacgtttcgc gtacgcggtc ggtggtgggg cgcagacctg
     8641 gactatcagg aaccgggagt ttacggcctc gccactgccc gccaataatg cggatttggc
     8701 cgctgccgga atgattcggt tttttcatga taaattgctc aatccgccag ataacacata
     8761 cttgcaggcg gtgatgtgaa ttaagttaag tgatagacta tttcatcatt tttttagctg
     8821 ctatgtacat agcgttaacg ctgtgccatg aagcaacagc gaggagtgta gtcgcaaatg
     8881 gcgaaagaaa aaaaacgtgg ctttttttcc tggctgggct ttggtcaaaa agagcagacc
     8941 ccggaaaaag agacagaagt tcagaatgaa caaccagttg tagaagaaat cgttcaggcg
     9001 caagagcctg tgaaggcttc tgaacacgcc gttgaagagc agccgcaggc gcatactgaa
     9061 gccgaggcgg aaacttttgc tgccgacgtt gtggaagtta ctgaacaggt tgctgaaagt
     9121 gaaaaagcgc agcctgaagc ggaagtcgtt gcacagccgg aaccggtcgt agaagaaacg
     9181 ccggagccag tggctatcga acgtgaagaa ctgtcgttgc cggaagacgt caacgccgaa
     9241 gcggtttcgc cagaagagtg gcaggccgaa gcggaaaccg tagagattgt cgaagcggcg
     9301 gaagaagaag cggctaaaga agaaatcacc gacgaagagc cggaggcaca ggcgctggct
     9361 gccgaagcgg cagaagaggc ggtaatggtg gtttctccgg cagaagaaga gcagccggtg
     9421 gaagaaatcg ctcaggagca ggaaaaaccg accaaagaag gtttcttcgc gcgcctgaaa
     9481 cgcagcctgt taaaaaccaa agaaaatctc ggttccggat ttatcagcct gttccgcggt
     9541 aaaaaaatcg acgatgatct gtttgaagag ctggaagaac agctgttgat cgccgatgtg
     9601 ggcgtggaaa ccactcgtaa aattatcacc aatctgacgg aaggcgcatc ccgcaagcag
     9661 cttcgtgatg ccgaggcgct ctatggcctg ctgaaagaag agatgggcga gattctggcg
     9721 aaagtcgatg agccgctgaa tgttgaaggt aaaacgccgt tcgtgatcct gatggtgggc
     9781 gtcaacggtg tgggtaaaac cacgacgatt ggtaagctgg cgcgtcagtt tgagcagcag
     9841 ggtaaatcgg tgatgctggc ggcaggcgat actttccgtg cggcagcggt tgaacagctt
     9901 caggtctggg gtcagcgcaa caatattccg gtgattgccc agcataccgg tgcggattcc
     9961 gcttctgtta tcttcgacgc cattcaggcc gctaaagcgc gtaacatcga cgtcctgatt
    10021 gccgataccg ccggacgcct gcagaacaaa tcgcacctga tggaagagtt gaagaaaatc
    10081 gtccgcgtga tgaagaaact cgacgttgaa gctccgcatg aagttatgct gactattgat
    10141 gccagcaccg ggcagaatgc ggtaagccag gccaaactgt tccacgaagc cgttggctta
    10201 accggcatca cgctaacgaa actggacggc acggcgaaag gcggggtaat tttctcggtg
    10261 gctgaccagt ttggtatccc tatccgctac attggtgtcg gcgaacgtat tgaggatttg
    10321 cgtccgttta aggcggacga ctttatagag gcactttttg cccgagagga ttaacaatga
    10381 ttcgctttga acatgtcagc aaggcttatc tcggtgggag acaggcgctg cagggcgtta
    10441 cgttccatat gcagccgggt gagatggcgt ttctgaccgg tcattccggc gcagggaaaa
    10501 gtaccctcct gaagctgatc tgtgggattg agcgtcccag cgccgggaaa atctggttta
    10561 gcggccatga catcacgcgt ctgaaaaacc gtgaagttcc gtttctgcgc cgccagattg
    10621 gcatgatttt ccaggatcac catctactga tggaccgtac tgtctatgat aacgtggcga
    10681 tcccgctgat tatcgccggt gccagcggtg atgacattcg tcgccgggtg tcggcggcgc
    10741 tggataaagt cgggctactg gacaaagcga agaacttccc tattcagctt tccggcggtg
    10801 aacaacagcg tgttggcatt gcccgcgcgg tggtgaacaa gcccgcggta ctgctggcgg
    10861 acgaaccgac tggtaacctg gacgacgcgc tgtcggaagg cattttacgt ctgtttgaag
    10921 agtttaaccg cgttggggta accgtattga tggcaacgca cgacatcaac ctgatttcgc
    10981 gacgttccta tcgcatgctc accctgagcg atggtcactt gcatggaggc gtgggccatg
    11041 aataagcgcg atgcaatcaa tcacattcgg cagtttggcg ggcgtctgga tcgcttccgt
    11101 aaatcggtcg gcggctctgg cgacggcggt cgtaacgcgc cgaaacgcgc gaaatcctcg
    11161 ccaaaaccgg taaatcgcaa aaccaacgtt ttcaacgaac aggtacgcta tgccttccac
    11221 ggcgcattgc aggatctgaa aagcaaaccg ttcgccacgt ttttaacggt gatggttatc
    11281 gccatctccc tgacgctgcc cagcgtctgt tatatggtgt acaaaaacgt taaccaggcg
    11341 gcgacgcaat attatccgtc accgcaaatc actgtttatc tgcaaaaaac gctggatgat
    11401 gacgccgctg cgggcgtggt ggcacagttg caggccgagc aaggcgtgga gaaagtgaat
    11461 tatctttctc gtgaagacgc actgggcgag ttccgtaact ggtctggttt tggtggtgcg
    11521 ctggatatgc tggaagaaaa cccgcttccg gcagtagcgg tggtgatccc gaaactcgat
    11581 tttcagggga cggagtcact gaatacgctg cgtgatcgta tcacgcagat taacggcatt
    11641 gacgaagtgc ggatggatga cagctggttt gcccgcctgg cggcgttgac cgggctggtc
    11701 gggcgcgttt cggcaatgat cggcgtattg atggtggcgg cagtgttcct cgtcatcggt
    11761 aatagtgtgc gcctgagtat ctttgctcgc cgtgattcta ttaacgtgca gaaactgatt
    11821 ggtgcgacgg atggtttcat cctgcgtccg ttcctgtacg gtggcgcatt gctgggattt
    11881 tctggcgcat tgttgtcact aattttgtca gaaattctgg tgctgcgttt gtcatcggcg
    11941 gttgcggaag tggcacaggt tttcggaacg aagtttgata tcaatggctt atcgttcgat
    12001 gaatgcctgc tattgctgct ggtatgctcg atgattggct gggtggcagc gtggcttgcc
    12061 acggtacaac atttacgcca ctttacgcct gaataataaa agcgtgttat actctttccc
    12121 tgcaatgggt tccgtagcag ggaaagagac cccgttgtct cttcccggta tttcatctct
    12181 atgtcacatt ttgtgcgtaa tttattcaca gcgttgcatt gaacttgtgg ataaaatcac
    12241 ggtctgataa aacagtgaat gataacctcg ttgctcttaa gctctggcac agttgttgct
    12301 accactgaag cgccagaaga tatcgattga gaggatttga atgactgaca aaatgcaaag
    12361 tttagcttta gccccagttg gcaacctgga ttcctacatc cgggcagcta acgcgtggcc
    12421 gatgttgtcg gctgacgagg agcgggcgct ggctgaaaag ctgcattacc atggcgatct
    12481 ggaagcagct aaaacgctga tcctgtctca cctgcggttt gttgttcata ttgctcgtaa
    12541 ttatgcgggc tatggcctgc cacaggcgga tttgattcag gaaggtaaca tcggcctgat
    12601 gaaagcagtg cgccgtttca acccggaagt gggtgtgcgc ctggtctctt tcgccgtcca
    12661 ctggatcaaa gcagagatcc acgaatacgt tctgcgtaac tggcgtatcg tcaaagttgc
    12721 gaccaccaaa gcgcaacgca aactgttctt caacctgcgt aaaaccaagc agcgtctggg
    12781 ctggtttaac caggatgaag tcgaaatggt ggcccgtgaa ctgggcgtaa ccagcaaaga
    12841 cgtacgtgag atggaatcac gtatggcggc acaggacatg acctttgacc tgtcttccga
    12901 cgacgattcc gacagccagc cgatggctcc ggtgctctat ctgcaggata aatcatctaa
    12961 ctttgccgac ggcattgaag atgataactg ggaagagcag gcggcaaacc gtctgaccga
    13021 cgcgatgcaa ggtctggacg agcgtagtca ggatatcatc cgcgcgcgct ggctggatga
    13081 agacaacaag tccacgttgc aggaactggc tgaccgttac ggtgtttccg ctgagcgtgt
    13141 gcgtcagctg gaaaagaacg cgatgaaaaa attgcgcgct gctattgaag cgtaatttcc
    13201 gctattaagc agagaaccct ggatgagagt ccggggtttt tgttttttgg gcctctacaa
    13261 taatcagttt ccccctccgg caaaacgcca atccccacgc agattgttaa taaactgtca
    13321 aaatagctat tccaatatca taaaaatggg gtatgtttta gcagagtatg ctgctaaagc
    13381 acgggtagct acgtataaaa cgaaataaag tgctgcacaa caacatcaca acacacgtaa
    13441 taaccagaag agtggggatt ctcaggatga acataaaggg taaagcgtta ctggcaggat
    13501 gtatcgcgct ggcattcagc aatatggctc tggcagaaga tattaaagtc gccgtcgtag
    13561 gcgcaatgtc cggtccggtg gcgcagtacg gtgaccagga gtttactggc gcagagcagg
    13621 cggttgcgga tatcaatgct aaaggtggta tcaaaggcaa caaactgcaa atcgtaaaat
    13681 atgacgatgc ctgtgacccg aaacaggcgg ttgcggtggc gaacaaagtc gttaacgacg
    13741 gcattaaata cgtgattggt cacctctgct cttcatcaac gcagccagcg tctgacatct
    13801 acgaagacga aggcatttta atgatcaccc cagcggcaac cgcgccggag ctgaccgccc
    13861 gtggctatca gctgatcctg cgcaccaccg gcctggactc cgaccagggg ccgacggcgg
    13921 cgaaatatat tcttgagaaa gtgaaaccgc agcgtattgc tatcgtccac gacaaacagc
    13981 aatacggcga gggtctggcg cgtgcggtgc aggacggcct gaagaaaggc aatgctaatg
    14041 tggtgttctt tgatggtatt accgccgggg aaaaagattt ctcaacgctg gtggcgcgtc
    14101 tgaagaaaga gaatatcgac ttcgtttact acggcggtta ccacccggaa atggggcaga
    14161 tcctgcgtca ggcacgtgcg gcagggctga aaacccagtt tatggggccg gaaggcgtgg
    14221 ctaacgtctc gctgtctaac attgcgggtg aatcagcaga agggctgctg gtgaccaagc
    14281 cgaagaacta cgatcaggtt ccggcgaaca aaccgattgt tgacgcgatc aaagcgaaaa
    14341 aacaggaccc aagcggcgcg ttcgtctgga ccacctacgc cgcgctgcaa tctttgcaag
    14401 cgggcctgaa tcagtctgac gatccggcgg agatcgctaa atacctgaaa gcgaactcgg
    14461 ttgatacggt gatggggccg ctgacctggg atgagaaagg cgatctgaaa ggctttgagt
    14521 tcggtgtgtt tgactggcac gccaacggca cggcgactga tgcgaagtaa tcattaatcg
    14581 gcaactttgg gttgccgcca aattgctaat atcgagtacg ttgcttcatg ccggatgcgg
    14641 cgtaaacgcc ttatccggcc tacaagattc aaagaaatca gtaaattgca gcacacattg
    14701 taggcctgat aagcgtagcg catcaggcaa tatcactttt gaaatcaaac ttgacgatta
    14761 atgcttctcc cagccaccct gttgtgccgt aaatcccagt gctcgcataa acgctgtcat
    14821 cacgccgcga tcttccacgc cagcatccgc catccaccaa catgaaacgt caggattgtt
    14881 acgcaaaacc tcttccagta gatattgccc cacaccgcga cgtcgggtga cttcccgcac
    14941 gcgtagagaa tccagtgccc cctcggtgcc gcttaaggtt acccgcacgg cagcgagcag
    15001 gcgctcgtta aaacgcgcgg cgtagatacg gtggttatcg tcaacctgta aagaggaagg
    15061 ggaatactcc ggccagatct tttgcaggtc aatccggtcc tggtcgctaa agttttctaa
    15121 tcgaatgatg gtcagcttca tggggaaccc gtgtaaatca caaaagtgga accagtgtag
    15181 cgaaataatt taatcggagg ctttctc
//
LOCUS       DAZFJL010000113        15142 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFJL010000113 DAZFJL010000000
VERSION     DAZFJL010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.113"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            54..1412
                     /gene="chbC"
                     /locus_tag="ACHJ8C_003488"
     CDS             54..1412
                     /gene="chbC"
                     /locus_tag="ACHJ8C_003488"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO5553280.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVHRNWVIKLPDSVPTSVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            1463..1813
                     /gene="chbA"
                     /locus_tag="ACHJ8C_003489"
     CDS             1463..1813
                     /gene="chbA"
                     /locus_tag="ACHJ8C_003489"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO5553281.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            1821..2663
                     /gene="chbR"
                     /locus_tag="ACHJ8C_003490"
     CDS             1821..2663
                     /gene="chbR"
                     /locus_tag="ACHJ8C_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO5553282.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDIPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            2766..4118
                     /gene="celF"
                     /locus_tag="ACHJ8C_003491"
                     /gene_synonym="chbF"
     CDS             2766..4118
                     /gene="celF"
                     /locus_tag="ACHJ8C_003491"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO5553283.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSMNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELNVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHYDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            4131..4889
                     /gene="chbG"
                     /locus_tag="ACHJ8C_003492"
     CDS             4131..4889
                     /gene="chbG"
                     /locus_tag="ACHJ8C_003492"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO5553284.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPTVAKFVAEEGIALRIDRQPLSNDG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELHVLTSASLKYAIAERGYRLGSYHDV"
     gene            complement(4936..7197)
                     /gene="katE"
                     /locus_tag="ACHJ8C_003493"
     CDS             complement(4936..7197)
                     /gene="katE"
                     /locus_tag="ACHJ8C_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO5553285.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSKNYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDTNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQVAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIANNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIAEADSADGSFMDELLTLMTAHR
                     VWSRIPKIDKIPA"
     gene            7380..7643
                     /gene="cedA"
                     /locus_tag="ACHJ8C_003494"
     CDS             7380..7643
                     /gene="cedA"
                     /locus_tag="ACHJ8C_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO5553286.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQENEVEE"
     gene            complement(7690..9081)
                     /gene="tcyP"
                     /locus_tag="ACHJ8C_003495"
     CDS             complement(7690..9081)
                     /gene="tcyP"
                     /locus_tag="ACHJ8C_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO5553287.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(9214..9804)
                     /gene="ydjM"
                     /locus_tag="ACHJ8C_003496"
     CDS             complement(9214..9804)
                     /gene="ydjM"
                     /locus_tag="ACHJ8C_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5553288.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(9968..10636)
                     /gene="hxpB"
                     /locus_tag="ACHJ8C_003497"
     CDS             complement(9968..10636)
                     /gene="hxpB"
                     /locus_tag="ACHJ8C_003497"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO5553289.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVEQVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLLSLTELTAK
                     DLLG"
     gene            10783..11319
                     /gene="yniB"
                     /locus_tag="ACHJ8C_003498"
     CDS             10783..11319
                     /gene="yniB"
                     /locus_tag="ACHJ8C_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO5553290.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNVFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PLICIAAGYVFFSLLGFI"
     gene            complement(11360..12220)
                     /gene="yniA"
                     /locus_tag="ACHJ8C_003499"
     CDS             complement(11360..12220)
                     /gene="yniA"
                     /locus_tag="ACHJ8C_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO5553291.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGRDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(12326..12616)
                     /gene="ghoS"
                     /locus_tag="ACHJ8C_003500"
     CDS             complement(12326..12616)
                     /gene="ghoS"
                     /locus_tag="ACHJ8C_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO5553292.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(12717..13646)
                     /gene="pfkB"
                     /locus_tag="ACHJ8C_003501"
     CDS             complement(12717..13646)
                     /gene="pfkB"
                     /locus_tag="ACHJ8C_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO5553293.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            13932..14690
                     /gene="ydiY"
                     /locus_tag="ACHJ8C_003502"
     CDS             13932..14690
                     /gene="ydiY"
                     /locus_tag="ACHJ8C_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO5553294.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(14743..14850)
                     /gene="yniD"
                     /locus_tag="ACHJ8C_003503"
     CDS             complement(14743..14850)
                     /gene="yniD"
                     /locus_tag="ACHJ8C_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO5553295.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(15021..>15142)
                     /locus_tag="ACHJ8C_003504"
     CDS             complement(15021..>15142)
                     /locus_tag="ACHJ8C_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553296.1"
                     /translation="AVHIAIHHNHHKTVETYYAAINVISQSLSFSADELKTYL"
BASE COUNT         3840 a         3528 c         3811 g         3963 t
ORIGIN      
        1 ttcataaatc aataccgcaa tatttaaatt gcggttttaa agggtatttt tctatgagta
       61 atgttattgc atcgcttgaa aaggtactcc tcccttttgc agttaaaata ggaaagcagc
      121 cacacgttaa tgcaatcaaa aatggcttta ttcgcttaat gccgttaacc cttgcggggg
      181 ccatgtttgt attaattaac aacgtttttc taagctttgg ggaggggtcg tttttttatt
      241 ccttaggtat tcgcctggat gcctcaacca ttgaaacact taatggtctg aaaggtattg
      301 gcggcaacgt atataacgga acattaggga taatgtcttt aatggccccg ttctttattg
      361 gcatggcgct ggcagaagag cgtaaagtcg atgcgctggc ggccgggttg ttatccgttg
      421 cagcatttat gaccgtcacc ccatatagtg tcggtgaggc ctatgcggtt ggcgcaaact
      481 ggttaggtgg ggcgaatatc atctccggga ttattattgg cctggtggtg gcagaaatgt
      541 ttacctttat tgttcatcgt aactgggtca ttaagttacc tgatagcgtt ccgacatcgg
      601 tttcgcgctc tttctcagca ttaattcccg gttttattat tctttccgtg atggggatta
      661 ttgcctgggc gttgaatacc tggggcacca acttccatca gatcattatg gataccatct
      721 caaccccact ggcatcgttg ggtagcgtgg taggctgggc ctatgtgatc tttgttccac
      781 tgctctggtt cttcggtatt catggcgcgc tggcgctgac cgcactggac aacggcatta
      841 tgacgccgtg ggcgctggaa aatatcgcga cctatcagca atatggttcc gtcgaagcgg
      901 cgctggcagc cggtaagacc ttccatatct gggccaagcc gatgctggac tcctttattt
      961 tccttggtgg cagtggtgcg actttaggcc tgatcctggc tatctttatc gcttctcgcc
     1021 gtgctgatta tcgtcaggtg gcaaaactgg cgctgccgtc cggcatcttc cagattaacg
     1081 aaccgattct gtttggtctg ccaattatca tgaacccggt gatgtttatc ccgtttgtac
     1141 tggtacaacc gattctggcg gcaatcaccc tggcagcgta ctacatgggc attattccac
     1201 cggtgaccaa tattgcaccg tggaccatgc caaccggtct gggagccttc tttaacacca
     1261 acggtagcgt cgccgcattg ctggtcgcac tcttcaatct tggtatcgca acgttaattt
     1321 atctgccctt tgttgtggtc gctaacaaag cacaaaacgc gattgataaa gaagagagcg
     1381 aagaagatat cgccaacgcc ctgaaatttt aattgatgcc ggtacgggta atcgtgccgg
     1441 taagaataga gaggaacgat gtatgatgga tctcgataat attcccgata cgcaaacgga
     1501 agctgaagag ctggaagaag tggtgatggg gcttatcatc aactccggac aagcgcgtag
     1561 cctggcgtat gcggcactga aacaggcgaa gcagggcgat tttgccgcag caaaagcgat
     1621 gatggatcag tcacggatgg cattgaatga agcgcatctg gtacagacga aactgattga
     1681 aggcgatgct ggcgaaggta agatgaaagt gagtctggtg ctggtccacg ctcaggatca
     1741 tttaatgacg tccatgcttg cgcgtgaact gattactgaa ttaattgagc ttcatgaaaa
     1801 actgaaggca taaggagtcg atgatgcagc cagtgattaa cgcgccggaa attgccactg
     1861 cccgggaaca gcagttgttt aatggcaaaa acttccatgt gtttatctac aataaaactg
     1921 agagtatcag cggactgcat cagcacgact attatgaatt tactctggta ttaaccgggc
     1981 gttatttcca ggagattaac ggtaagcgcg tgttactgga acggggcgat tttgttttta
     2041 ttccgttagg ttcgcaccat caaagttttt atgaatttgg tgccacgcgt atattgaacg
     2101 ttgggatcag taaacgcttt tttgagcagc attacctgcc gttgttgcct tattgctttg
     2161 tagcttcgca ggtataccgg accaataacg cttttctcac ctatgtggaa acagtgattt
     2221 cttcattgaa tttccgcgaa acagggctgg aagagtttgt tgagatggtt actttttatg
     2281 tcattaaccg tttacgtcat taccgcgaag aacaggtgat tgatgatata ccgcagtggc
     2341 tgaaaagtac ggtagaaaaa atgcatgata aagagcagtt tagtgaatcg gcgctggaga
     2401 atatggtgac gttgtcagcc aaatcacagg aatatttgac gcgagcgact caacgatatt
     2461 atggtaaaac gccaatgcag attattaatg aaatccgtat taattttgcc aaaaaacaac
     2521 tggaaatgac caactattca gtgacggata ttgcgtttga ggccggttat agtagcccga
     2581 gcttgtttat taaaacgttt aagaaattaa cgtcctttac gcctaagagc tatcgtaaga
     2641 aattgactga atttaatcag taagtcgtta tacctgacaa ttcacatatc tgtcctgttg
     2701 ctggattatt tatgtccggg ggcagatatg ccagatatca gtattctgta ctgaagggag
     2761 aaattatgag ccagaaatta aaagtcgtca ctattggtgg cgggagcagc tataccccgg
     2821 agttactgga aggatttatt aagcgttatc acgaattgcc ggtcagcgaa ttatggctgg
     2881 tggatgtcga aggtggtaaa gcgaaactgg atattatttt tgatctctgc caacggatga
     2941 ttgataacgc tggcgtcccg atgaagcttt ataaaacgct ggatcgccgc gaagcattga
     3001 aagatgctga tttcgttact acccaactgc gcgttggcca attaccggcg cgggaactgg
     3061 atgaacgtat tccattaagt catggttatc ttggtcagga aactaacggc gcgggcggtc
     3121 tgtttaaagg tctgcgtacc attccggtga tttttgacat cgtaaaagat gtcgaagaac
     3181 tatgtccgaa tgcatgggtg attaacttca ctaacccggc gggaatggtc actgaagccg
     3241 tttatcgtca taccggattt aaacgcttta tcggtgtgtg taatattccg atcggcatga
     3301 agatgtttat tcgcgatgtt ctgatgctga aagacagcga tgatttatct atcgatctgt
     3361 tcggcctcaa ccatatggtg ttcattaagg atgtgctggt aaatggcaaa tcacgctttg
     3421 ccgaattgct tgatggtgtg gcatccgggc agttaaaagc atctggcgtt aaaaatattt
     3481 tcgatctgcc atttagcgaa ggcttaattc gttctatgaa tctgttgccg tgttcttatt
     3541 tgctttatta cttcaagcaa aaagagatgc tggctattga aatgggcgaa tactacaaag
     3601 gcggcgcacg agcgcaggta gtacagaaag tcgagaaaca actttttgag ctatataaaa
     3661 acccggagtt gaacgttaag ccgaaagaac tggaacagcg cggtggggct tattattctg
     3721 atgctgcgtg cgaagtgatc aacgctatct ataacgacaa gcaagcagaa cattacgtta
     3781 atatcccgca tcatgggcat attgataata ttccggcaga ctgggcggta gaaatgacct
     3841 gtacgctggg gcgcgatggc gcgacgccac atccgcgcat tacgcattac gatgataaag
     3901 tgatggggct gattcacacc atcaaaggct tcgagattgc tgccagcaac gccgcactta
     3961 gtggagaatt taacgatgtc ttactggcgc taaaccttag tccgttggtg cattccgatc
     4021 gcgatgctga gctgctggca cgcgagatga ttctggcgca cgagaaatgg ctgccaaatt
     4081 ttgccgactg catcgcagag cttaaaaaag cacattaacc gaggctgatt atggaacgct
     4141 tactgattgt taatgccgat gattttggct taagcaaagg tcagaactac ggcattatcg
     4201 aggcctgtcg caatgggatt gtcacgtcga cgacggcgct ggtgaatggg caggctattg
     4261 accatgcggt gcagttgagc cgtgatgaac cgagtctggc catagggatg cactttgtcc
     4321 ttactatggg caagcctctg acagctatgc cggggttaac ccgcgatggt gtgctgggaa
     4381 aatggatctg gcagttggca gaagaagatg ctttaccgct ggaagaaatt actcaggagc
     4441 tcgccagtca gtatttgcgt ttcattgagc tatttggacg caaacctacg catcttgata
     4501 gccatcatca tgtgcatatg ttcccgcaga ttttcccgac tgtggcaaag tttgtggctg
     4561 aagaggggat tgcgttgcgc atcgaccgtc agccgttatc taacgatggt gatttaccgg
     4621 ctaatctgcg cagttcgcag ggattcagta gcgcgtttta tggtgaagag attagcgagg
     4681 cactgttcct gcaagtgctt gatgattcca gccatcgcgg ggaacgttcg ttggaagtca
     4741 tgtgccatcc ggcgtttgtc gataatacga ttcgccagag tgcttattgc ttccctcggt
     4801 taacggaact gcatgtgctg acttcagcgt cgttgaaata tgcgattgct gagcgtggtt
     4861 atcggttggg tagttatcat gatgtgtaag taaggtggta aggtcactcc ccagggggcg
     4921 tgaccttatc catatttacg ccgggatttt gtcaatctta ggaatgcgtg accacacgcg
     4981 gtgtgctgtc atcagcgtta acagttcatc cataaaacta ccatcggcgc tgtcagcttc
     5041 cgcaatccct tcttcaccct ggtcagcgac cttgattgtt gctttaaact tgcgcgcgtc
     5101 tcctgccagc gcaatcggtt taaggtgttt gtaggcttcc atcagatagt agttggcatc
     5161 gccgttgtta gcgatatccg caatattgcc gcaagggaca atgactgcat cgaccgtcag
     5221 cgaaggcgca cctgcaaagg tagcagctat aggcaacacc gtaccgtcat ccgcagtcac
     5281 ttcccccatt cgggagtaga gcagtttggc atgaacgcct ttggccttca gtgccttgag
     5341 aatggccaga aggtctgccg atctcacttc atcattaagc aaaattgcta ccacgcgacc
     5401 tttcacatca ccgtcaggaa tggcgtacag acttaaggat ggatcctttt tcagaccgtt
     5461 gacgtccgga ggcggggtga tattcaactg gtcgtcagtc agttcgatac cgagattttt
     5521 cgccaccgcc tgggccagag tgagatcaat atgtgccagt tggtcaacaa cgcgctcacg
     5581 aatatacgga cgaactactt tgcttaactc aaaactgaaa ccatcgacaa tatggcgctg
     5641 ctcgaatggc gtctgactta gccagaacag acgcggatgg gaataatatt cgccaaacga
     5701 tggactgcgc tcgcgaactt tattgccttc cacgcgttcc tggtatgatt caaaaccgcc
     5761 gcgtttcggc cccggcggtg tttcgcgcgg ccagttatcg ttgatcgagt tcggttcgta
     5821 attcgccggg ttagtgtcga tccccatacg atgcatgccg tcacgctgga aattatggta
     5881 agggcaggtc gggcggttaa tcggaatttc atggaaattc ggcccaccaa gacgactgat
     5941 ttgtgtatcg gtataagaga acaaacgccc ctgcaataac gggtcgttgg tgaaatccag
     6001 accggggaca atatgccctg gatggaaagc cacctgttcg ttttcagcaa agaagttatc
     6061 cggattacga ttgagcacca ttttgccgac acgctgaacg ggcaccaatt cttccgggat
     6121 aagtttggtt ggatcgagaa gatcgaagtc gaacttgaat tcgtcttctt caggaatcaa
     6181 ctggaagccc agttcgtatt caggaaaatc gcctgcttca atcgcttccc acaactcgcg
     6241 gcggtggaag tccgggtcac gtccggttag tttttgtgct tcatcccaaa cgagtgaggc
     6301 tttacctgcc agtggtttcc agtggaaacg tacgaacgtt gccttccctt cggcattaat
     6361 caggcggaag gtatgaatac cgaagccttc catggtgcgg taactgcgcg ggataccgcg
     6421 atccgacatc gcccacatca cgttgtgcag agtttcaggt tgcagagaaa cataatccca
     6481 gaaagtatcg tgggcgcttt gcccttgtgg aattgcccag tgtggttctg gttttaccgc
     6541 atgaacaaaa tcggggaatt tatgcgcatc ctggataaag aagattggcg tgttattgcc
     6601 aacgaggtca aaaataccct cttcagtata gaacttggtg gcaaagccac ggatatcacg
     6661 cacggtatcg gcagagccag cgccgccctg aacggtagag aacctaacaa atactggggt
     6721 gattttgttc gtatctgaga ggaaatctgc tttggtgata tcgcttaagc ttttatatgg
     6781 ctggaaataa ccgtgagctg ctgatccgcg tgcatgaaca atacgttccg ggatgcgctc
     6841 atggtcaaag tgagtgattt tctcgcgcag aataaaatct tccagcagag ttggaccacg
     6901 gctaccggca cgcagtgagt tttgatcgtc ggcgatgcgc acgccctgat tagtggtcag
     6961 cgcataattt ttactgcctt tgcgtacgtc ttccagagaa ttaagttttt cgttacgcgt
     7021 atcaggggct ttcaggctcc ctggggcggt aggttgtgca ccaggcggtg ttggttcagc
     7081 cgctggacga tgagatccgt cttcaggtgc cagtgagtcc atccccggtt tcgcttcgct
     7141 ggaatcgtgt agtggtgact ggtgctgatg tgggttcttt tcgttatgtt gcgacattga
     7201 actcgtctcc ttaaattatt actgaaaggg ccgctgtttt ggtcaaaaaa ccctctaact
     7261 atagaccacc agccagatcc cgcttcggaa actattctgg aaacggtgtt atgaaataca
     7321 cgcaaagcgg gacgtacagg ggctaaatcg gctaaactac agtttttctg atttttaata
     7381 tgcgtttagt gaagccagta atgaagaaac cgctccgtca gcaaaaccgc cagattatta
     7441 gctatgtccc acgcacggaa cccgcgccgc cagaacatgc gataaagatg gattcgtttc
     7501 gagatgtctg gatgctgcgt ggcaaatatg ttgcgtttgt actgatgggg gagtcatttc
     7561 tgcgctcacc ggcgtttact gtgcctgaat ccgcccagcg ttgggcaaat cagattcgtc
     7621 aggaaaatga ggtcgaagaa taaaaaaaca cctgtcggga tgacaggcgt ttaacgtaac
     7681 aaatcaggat taacggtgtg ccagttcggc gtcgtcttcg ctatccagaa tggctttatc
     7741 ggtttgcttc agccactggc tggtcagcgt gccagcggtc atcgagccac taacgtttag
     7801 cgccgtacga cccatgtcga taagcggttc aacggagatt aacaatgcca ccagcgttac
     7861 cggcaggccc atcgcaggca gcacaatcag tgcggcgaaa gttgcaccac caccgacacc
     7921 ggcaacgcct gcggaactaa cggtgacaat accgaccagc gtcgcaatcc acatcgggtc
     7981 cagtgggtta atgcccaccg taggcgcaac catcaccgcc agcattgccg gatacaaacc
     8041 ggcacaaccg ttctgaccaa tggtcgcacc gaaagaggcg gcgaaactgg cgatggattc
     8101 aggaacaccc agacgacgcg tttgtgcttc cacattcagt gggatagacg cagcactgga
     8161 acggctggta aaggcaaacg tcagtactgg ccatacctta cggaagtact tcagcgggct
     8221 cacgccatta atgcccagca gaaggccatg cactgcaaac ataatcagca gaccgaggta
     8281 ggacgcaaca acgaaacttc ccagtttgat gatgtcttgc aggttagaac ctgcaaccac
     8341 tttggtcatc agcgccagaa caccgtaagg ggtcaactgc atgaccaggc gaaccagttt
     8401 catcacccag ctttgcaggg tatcgatagc ggttaagacg cgttcacctt tcggcgcatc
     8461 atccttcagc agtttcagcg cagctacgcc gaggaatgcg gcaaaaatta ccacgctgat
     8521 aattgacgtc ggattggctc cggtcagatc ggcaaacggg tttttcggga taaaggacaa
     8581 gaccagctgc ggaacgctca ggtcagagac tttaccaaca tagttacttt cgatggcgtt
     8641 cagacgtgca gtttctgcac caccctgaac cagaccttca gccgtcaaac caaacaggtt
     8701 ggtgaccagc acaccgacca gcgctgcaat cagcgtggta aacaaaagcg taccgatggt
     8761 cagaaaactg attttgccta actgagatgc gttatgcaga cgggcaaccg cgctcagaat
     8821 agaggcgaag actagcggca taacgatcat ttgcagcagt tgaacatagc cgttaccaac
     8881 aatgttaaac cactgtacag aatctttaag tacctggctg tcagaaccat aaatggtatg
     8941 cagggcaagg ccaaaaacca cacccatcac aagacccacc agcacttttt tcgccagact
     9001 ccactgtttg tggcgggttt gagccagcgc aaacagcagt acaacgaaca ccacgatgtt
     9061 cgcaattaat ggaaagttca tccccgttct cctgatctta ttatttctat cactgcgtaa
     9121 cagtcatagt gttggcgcaa gattaacaga actgtgagct ggctcttata ttcaaatgga
     9181 attgcttatt ccagaaaggc gatttttgcc tttttagtat tcaacctgat gcttaataag
     9241 ccgatgaaac tggatctgta aggtattgat catttgcgat gaccaacgaa cagcgctgtt
     9301 ttcgggtaat gaatggggca tccataccga ccagacaaat aatgccatgc agataaaacg
     9361 ttccagttgg ttgccctttt ggggaaccag gataggcaag cggaaacgcc agcggcatgg
     9421 ccagagcagg ggaacaccgg cgggtgtcag catatcggca agtatgtggc tcaaataacc
     9481 cagcaccata ccctgcaacg catcagccgg aatgaaccag ctttcaggaa ccttaaggta
     9541 aaaggttgcc agcagcgcaa ataccgccag cagactgtgg gtaaaaccac gatgcccaaa
     9601 agcgcgggcg atcggttttg atatccactt taatcgctgc ccaagaaacg actttgggtg
     9661 atcgatgtcc ggtaacaaac acgtcaggat tgcggaaggg acaatatgcc accagtcacc
     9721 ctgtgccagc acgggcgtca gctcggcatt tttggcaaat accgcacaag caatagaaaa
     9781 gagaaggtga ccttccgccg tcatgataaa actcactaaa ctgtcgatac gtacagtata
     9841 ggatttttat acagtgcacg gaagtggtga cggtgtaact ctttctctta aattggcgat
     9901 taatccgtca gttgaatgca aattcagata aagaaccagg ggcagtgtga tcactgcccc
     9961 ctggtcatta accgagaagg tcttttgcgg tgagttctgt cagcgataag agtttgacgt
    10021 ttgctaatac aaaacgtgga tcattttgcg cttctggcgc aggaacgacg atggaacgca
    10081 tgcgggctgc tttagaggcg atcatgccat ttaccgaatc ttccagtgcc acgcaggtca
    10141 gagggtcaac ccccagtttt gctgcgcagt cgagatatac ttgcggatgc ggcttgctgt
    10201 aaggcaattt ttcagccgag gcgagggcat cgaaactgtc gcgtaaatca aacatggtca
    10261 atactttttc cagcatatgc agcggtgacg cggaggccag ccccaccaat aaaccttgtt
    10321 ctttgcataa cgcaacggct tcacgcacac ctggcaataa tggacgtgtc tcttcaacca
    10381 gtgaaatggc acgggcaata acttgttcta ctacttcctg acggcttggc ccattccatg
    10441 gttgccgggc gtaccaaaga tcgaccacca tatcgatgcg taaacctaag gtgtccggca
    10501 gctcgttacg acgggagata tccaccccca ggcttgccat cacatccagt tcggctcgat
    10561 cccataaagg ttctgagtcg ataagtaatc catccatatc aaaaattgca gcaagaatct
    10621 gacgcggggt tgacattaca acctctcctt tagagcatta tatgtaaggt attaagccgt
    10681 gcttatgatt aagcgcctat catatcgcct ttaaagaatg ggcgaaaaca gatatctaca
    10741 ggtaaactaa gtgaaacgac gcgtcttaat caaggagaac tcatgacgta tcaacaagct
    10801 ggacgcattg ctgttttgaa aaggattttg ggttgggtga tttttatacc tgcattgatc
    10861 tctacattga tttctttgct gaaatttatg aatacccggc aggaaaacca ggaaggcatt
    10921 aatgcggtca tgctcgactt tactcatgtc atgatcgata tgatgcaggc gaatacgcct
    10981 tttttgaatg tgttctggta caactctccg acgcccaatt ttaatggtgg cgtgaacgtt
    11041 atgttctggg tgattttcat cctgattttt gtcggactgg cgttgcagga ttccggagcc
    11101 cggatgagtc gccaggcgcg cttcttgcgg gaaggtgttg aagatcaact cattctggaa
    11161 aaagccaaag gggaagaggg gctaacgcgt gaacaaattg agtcacgtat tgttgttccg
    11221 caccatacta ttttcctgca gtttttctcg ctgtatatcc tgccgcttat ttgtattgct
    11281 gcaggttatg tgttcttttc tctgcttggg tttatttaat caaaaggccg cacggagcgg
    11341 ccattatcct caacccatat catgctgcta ataatctatc cagtgactgc tgagcaataa
    11401 ccaaatgctg accgccaaat aaccttgcac gatttaacag cgtgtagagt tggtaaaccg
    11461 gttgacgttc aaggaaatcg gcaggtagcg gggatactga ctgatagccg tcatagattt
    11521 gtggcggctg ttcagtatgc agcggtaaca tcgccaggtc gcactctcgg tcaccccagt
    11581 aacaggccgg gtcgaaaatg tacgggccat ccggacccag cgcacagttg ccggaccata
    11641 aatcgccgtg caacagagaa ggctgcggtt gatgcgaggc gagacgctgc tgaatatgct
    11701 cgacgatggc atcgatattg ccgaaagcga tccctttttc tgccgccagt tccagctgcc
    11761 agccaatccg ttgttcagca aaaaaagttg accagcgacg ttgccaggtg ttgggctgtg
    11821 gagttgtgga gagcgcgtta tcgaaatcga ggccaaattg tggttggtcg ctccattgat
    11881 gtaaacgcgc aatttgctga ccaagaataa atgcactatg cgcatccagc gggcgaggtg
    11941 ggagataatc catcaccaga aaactgtagt cacggtcagc gccaactgcc caaaccttag
    12001 gcacggtgac ggttttacta cgcgacagta actccagttg gtcggcttcg gcggtaaaac
    12061 cgggaagcag ttccctttca tcacatttga cgaaaaagtc acgtcctgca tagcgcaaat
    12121 gccatgcggc atggacttct ccgccaggca gttcattacg cagttcgatt tcgccttcac
    12181 ctaactgctc gctcaaaaga cgactaattg cctgccacat gatgtctctc ccatgttgtc
    12241 tgccagatca taaggttagc gcaagaatgc ggtgtaaaaa cacgagctaa cgcacaccac
    12301 agactgatta ttgcccaaaa accatttatt ttctgttgct attccattcc tcccaggtcg
    12361 tgatggtgat ttcaggatct ttgccggttg cactttgggt aagccccgaa accagttctc
    12421 taatttcttc ttcactttgg gtactgataa gtccaaaagt gttagtcccc agctcatgga
    12481 tattgccatc atcatccgtc atggtgagca aaaaaccgtc gcgagtcagg taattattca
    12541 actcgttaat ttcagtcaat gtatcctcat gcaacatgac ggttattaca taacggacaa
    12601 gatcgccact ggccatagtt cacccctttg ttatcacatg acttttgagc atagtcggag
    12661 aaacgcgttg ccgacaggtt ggtggtgatt cccccaatgc tgggggaatg tttttgttag
    12721 cgggaaaggt aagcgtaaat tttttgcgta tcgtcatggg agcacagacg tgttccctgg
    12781 ttgagtgtgg ctgcactccc cgcagctacg ccaaaacgga ccatttcttc aagagaggca
    12841 ttttctgcca gtttcagtgt catcgcgccg accatgctgt caccagcgcc aacggtactc
    12901 tggcttttta ccggtggtgg caccacctga atgcagtttt cactatcaac gcccaacgcc
    12961 ccttgtggac ccagggaaac gacaacccgt ttggccttgc cgctattaac aatttcctgc
    13021 gcggctttgc ggacatcgtc tggctgggtg agttcgcgat tcaccagcgc actgagttct
    13081 ttttggttag gcttaaccaa ctcgatgtta ccaatcgcca gtgctgcact taacgcttcg
    13141 ccagaactgt cgatgatgca gcggatccct tgtttttgcg cagcggaaat cagttgggtt
    13201 aatttttcca gcttcacacc tggcggcagg cttccgctta tgaccagaat ggccccggat
    13261 tcaatttcca gaacttgctc ttcaagctgg cgaaactcat cttcatttaa tgccgcgcct
    13321 ggcataacaa aacgatactg ctcaccgctt gcttccacat gtacgtgtaa attctgccgg
    13381 gtccagtctt tggcttctac agtagcgacg gggacatttt catctgccaa cagtgaaacc
    13441 agatgttcgc cggtcgcacc acccgccggg aagatcgctg tggcactgcc tccaagatgg
    13501 gcaatggcgc gggcgacgtt gatgccgccg cctccgggtt cgaataccgg tgcggtacag
    13561 cgcagttttc cttcgggata aatttgcggg gtaattgttg cgctatcaag agagggcgca
    13621 agtgtcaacg tatagatacg taccatcatt tcctcctata ggctgatttc agtctggcac
    13681 cgaatcagcg gaaagtgaag aaattaacaa tatgatttat tggagcttta cttaaaattt
    13741 tgcagataaa tatatataaa taaaaatctg ttttcgccac atgcttattc aaaaaatgaa
    13801 ataagaatac attgctaagt tattcgcgcc tttttataat tcgttacctt tccagggacg
    13861 ctaatcttta tccttaagaa agtttccggg accgcgatgg tctctttttt tgttgtacgg
    13921 actcttgata aatgaagctt ttgaagacag ttcctgccat cgttatgttg gcggggggca
    13981 tgtttgcgtc actgaatgca gctgccgatg attccgtttt tactgtcatg gacgatcctg
    14041 ccagcgctaa aaaacctttc gaaggtaatc tgaacgcagg atatcttgca caatcaggca
    14101 atacgaaaag ctcctcactt acggctgaca ccaccatgac ctggtatgga caaaccactg
    14161 cctggtcgct gtgggggaac gccagtaata cttcttctaa tgatgagcgt tcttcagaaa
    14221 aatatgcggc gggcgggcgt agccgtttta atttaactga ttatgactat ttatttggtc
    14281 aggcaagctg gctgacagac cgttataacg gctatcgaga gcgcgatgtg ttgaccgcgg
    14341 gttatggtcg ccagtttttg aatggcccgg tacacagttt ccgctttgaa ttcggccctg
    14401 gtgtgcgtta cgacaaatat accgacaatg ccagtgaaac ccagccgctg ggctatgcct
    14461 ccggggctta tgcatggcag ttgactgaca acgctaaatt tacgcagggt gtttcggtct
    14521 ttggcgcgga agacacaacg ctgaactccg aaagcgcatt aaatgttgct attaatgaac
    14581 attttggact gaaggtggct tacaacgtta catggaactc tgaacctcca gagtctgcac
    14641 cggaacatac cgatcgtcgg acaaccttat cgttgggtta cagcatgtaa aattatctgc
    14701 cggaccgtgt tatgcggtct ggctgttttc gtttgtcact gctcagcccc aaatcattgc
    14761 agcccaacgt agcaacaaca tagcgccaca gattgccagt agcaccacca ccatcttcca
    14821 gtgtttttta tcaaagcgtt tagtcggcat accagtgtcc tgcatatctc ttgtgcgtga
    14881 taacggagtg taacaaaagg gacaggcatc aagggatttt ttgttattac agtcgtcggg
    14941 tggcgttgta cacctggtca ctgtttctgc attttatgaa aaaaattatg tatcgatata
    15001 caaatatcat aaatagcggg ttataaatac gtctttagtt catcagcact aaaactcagg
    15061 ctttggctga ttacatttat agcagcataa tatgtttcta cagttttatg atgattatga
    15121 tgaatggcta tatgaacagc tg
//
LOCUS       DAZFJL010000114        15139 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFJL010000114 DAZFJL010000000
VERSION     DAZFJL010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.114"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            1..976
                     /locus_tag="ACHJ8C_003505"
                     /pseudo
     CDS             1..976
                     /locus_tag="ACHJ8C_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533661.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            1320..2567
                     /gene="mdtA"
                     /locus_tag="ACHJ8C_003506"
     CDS             1320..2567
                     /gene="mdtA"
                     /locus_tag="ACHJ8C_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO5553297.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLIALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEDKATSREYAKKGARS"
     gene            2567..5689
                     /gene="mdtB"
                     /locus_tag="ACHJ8C_003507"
     CDS             2567..5689
                     /gene="mdtB"
                     /locus_tag="ACHJ8C_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO5553298.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            5690..8767
                     /gene="mdtC"
                     /locus_tag="ACHJ8C_003508"
     CDS             5690..8767
                     /gene="mdtC"
                     /locus_tag="ACHJ8C_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO5553299.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDDTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRARLPELQSTIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLVISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRVL
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRKKLAKEPGANLFLMAVQDIRVGGRQANASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQEDNGAEMNLIYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQAVTE"
     gene            8768..10183
                     /locus_tag="ACHJ8C_003509"
     CDS             8768..10183
                     /locus_tag="ACHJ8C_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5553300.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSQLAIAGLVAVGVVALVLYLLHAQNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFVF
                     ARVPSDTHQNVAISRRKRSVQ"
     gene            10180..11583
                     /gene="baeS"
                     /locus_tag="ACHJ8C_003510"
     CDS             10180..11583
                     /gene="baeS"
                     /locus_tag="ACHJ8C_003510"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO5553301.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLITMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSLEHDNNEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPVPPDGTRRPIMVNGAEVGAVIASPVERLTRNTDINFD
                     RQQRQTSWLIVALSTLLAALATFPLARGLLAPVKRLVDGTHKLAAGDFTTRVAPTSED
                     ELGRLAEDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TLASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     LSITVELPLERDLQREV"
     gene            11580..12302
                     /gene="baeR"
                     /locus_tag="ACHJ8C_003511"
     CDS             11580..12302
                     /gene="baeR"
                     /locus_tag="ACHJ8C_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO5553302.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            12482..12814
                     /locus_tag="ACHJ8C_003512"
     CDS             12482..12814
                     /locus_tag="ACHJ8C_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO5553303.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKVNGT
                     SQTVKDNT"
     gene            12961..14322
                     /gene="yegQ"
                     /locus_tag="ACHJ8C_003513"
     CDS             12961..14322
                     /gene="yegQ"
                     /locus_tag="ACHJ8C_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO5553304.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGELAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(14822..>15139)
                     /locus_tag="ACHJ8C_003514"
     CDS             complement(14822..>15139)
                     /locus_tag="ACHJ8C_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553305.1"
                     /translation="VKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDATHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         3487 a         4038 c         4054 g         3560 t
ORIGIN      
        1 agagttcgga ttaggcagag acagacgtat agcaatcact gaagctatac aggccaatat
       61 tgagttgcta tccgggaaca ggcgtgagtc actgatagac agaattaaag gcgctgacgc
      121 aatcactctt catgcgtggc ttgaccgata tgaaacaatc ctcagcgaga ggggtatcag
      181 gccgaaaact ctactcgact acgccagcaa aatcagggca atccgaagaa aattgccgga
      241 caaaccgctc gctgacatat caacgaaaga ggtggcagca atgctaaaca cctacgtagc
      301 agaaggtaaa gcggcttccg caaaattaat caggtcaacc cttgttgacg tttttcgtga
      361 ggcaatagcc gaggggcatg tggctacgaa tccggtaaca gcaacccgca cagcaaagtc
      421 agaagtaagg cgctcaaggc tgacagctaa tgagtatgtc gcgattcacc atgcagccga
      481 acctctccca atctggctga ggcttgcgat ggatttggcc gtcgttacag gtcagagagt
      541 cggcgatttg tgcaaaatga aatggtcaga cataaacgac aaccatcttc acattgaaca
      601 gggtaaaaca ggggctaagc tcgccattcc gctgacgcta acgattgacg cgctcaatat
      661 ctcattggcc gatacactat agaaatgcag ggaggtcagc ggcagtgaaa caataattgc
      721 atcaacgcat cacgaaccgc tttccccgaa aacagtatct aagtatttta caaaggcgag
      781 aaatgcatct ggactctcat ttgatggaga cccgccaaca ttccatgaac tgcgtagcct
      841 gtcggcgagg ctataccgga atcagattgg cgataagttt gctcaacgtc ttctcgggca
      901 taaatcagat tcaatggcgg cgcggtatag ggacagccga gggagagaat gggacaaaat
      961 tgaaatcaac aaatgatttt attttgatca ataatgacct atacactgta atttattgat
     1021 aattaaagat attttaaata tgcaacttac cttatttaag gccccttcct cgggaggggt
     1081 tttcccgttt cagcgtcccg ctgaaatcat cggcttacct cctttcgcca tgcacgtagt
     1141 ctatcgctaa cgcgtagata aaatagtttc atgtgttatt actggatgcg tgctcgcaaa
     1201 tgtgcccgcc attcagacga ttcccgatag tgtttcataa ttcctccatt tttctccctt
     1261 attggctggc tacactagta tcattccgcg aaacgtttca ggaagagaaa ctcttaacga
     1321 tgaaaggcag ttataaatcc cgttgggtaa tcgtaatcgt ggtggttatc gccgccatcg
     1381 ccgcattctg gttctggcaa ggccgcaatg actcccggag tgcagcccca ggggcgacga
     1441 aacaagcgca gcaatcgcca gcgggtggtc gccgtggtat gcgttccggc ccattagccc
     1501 cggttcaggc ggcgaccgcc gtagaacagg cagttccgcg ttacctcacc gggcttggca
     1561 ccattaccgc cgctaatacc gttacggtgc gcagccgcgt ggacggccaa ctgatagcgt
     1621 tacatttcca ggaaggccag caggtcaaag caggcgattt actggcagaa attgacccca
     1681 gccagttcaa agttgcatta gcacaagccc agggccaact ggcaaaagat aaagccacgc
     1741 ttgccaacgc ccgccgtgac ctggcgcgtt atcagcaact ggcaaaaacc aatctcgtat
     1801 ctcgtcagga actggatgcc caacaagcgc tggtcagtga aaccgaaggc accattaagg
     1861 ctgatgaagc aagcgtcgcc agcgcgcaat tgcaactcga ctggagccgt atcaccgcac
     1921 cagttgatgg tcgcgttggt ctcaagcagg ttgatgttgg taaccaaatc tccagtggcg
     1981 acaccaccgg gattgtggtg atcacccaaa cacatcctat cgatctggtc tttactttgc
     2041 cggaaagcga tatcgccacc gttgtacagg cacaaaaagc cggaaaaccg ctggtggtag
     2101 aagcctggga tcgcaccaac tcgaagaagt taagtgaagg cacgctgtta agccttgata
     2161 accaaatcga tgccactacc ggtacgatta aagtgaaagc tcgctttaat aatcaggatg
     2221 atgcgctttt ccctaatcag tttgttaacg ctcgcatgtt agtcgatacc gaacaaaacg
     2281 ccgtggtgat ccccaccgcc gcactgcaaa tgggcaacga aggccatttt gtctgggtac
     2341 tgaatagcga aaacaaagtc agcaaacatc tggtgacacc gggcattcag gacagtcaga
     2401 aagtggtgat ccgtgcaggt atttctgcgg gcgaccgcgt agtgacggac ggcattgatc
     2461 gcctgaccga aggggcgaaa gtggaagtcg tggaagccca gagcgccact accccggaag
     2521 ataaagccac cagccgcgaa tacgcgaaaa aaggagcgcg ctcctgatgc aggtgttacc
     2581 cccgagcagc acaggcggcc cgtcacgcct gtttattatg cgtcctgtcg ccaccacgct
     2641 gctgatggtg gcgatcttac tcgccgggat tatcggttat cgcgccctgc ccgtttcggc
     2701 gctgccggaa gtggactatc caaccattca ggtggtcacg ctttatccag gtgcaagccc
     2761 ggatgtcatg acctctgccg ttaccgcgcc gctcgaacgc cagttcgggc agatgtctgg
     2821 cctgaaacag atgtcgtcgc aaagttccgg cggtgcgtca gttatcactt tacagttcca
     2881 gctaacattg ccgctggatg tcgccgagca ggaagtgcag gccgcgatta acgctgcgac
     2941 caacctgcta ccgagcgatc tgcctaaccc gccggtttac agcaaagtga acccggcaga
     3001 tccaccgatc atgacgctcg ctgtcacctc aaccgccatg ccgatgacgc aggtggaaga
     3061 tatggtggaa acccgcgtcg cgcagaaaat ctcgcagatt tccggcgtag ggctggtcac
     3121 gctttccggc ggccagcgtc cggctgttcg cgtcaaactt aacgctcagg cgattgccgc
     3181 cctcggcctg accagcgaaa ccgtacgcac agctattacc ggcgctaacg ttaactcggc
     3241 aaaaggtagc ctcgacggcc cttcccgcgc ggtcacgctt tccgctaacg accagatgca
     3301 atccgccgaa gagtatcgcc agctaatcat cgcctaccag aacggcgcac cgattcgtct
     3361 gggcgatgtc gctactgtag agcaaggagc agaaaacagc tggctcggcg catgggctaa
     3421 caaagaacag gccattgtga tgaatgtcca gcgccagccc ggtgctaaca ttatctccac
     3481 cgccgacagc atccggcaga tgctaccaca gctcaccgag agtctgccga aatcggtgaa
     3541 ggtgacggta ctttctgacc gtaccaccaa tatccgcgca tcggtcgacg atacccagtt
     3601 tgagctgatg atggctatcg cgctggtagt catgattatc tacctgtttt tgcgcaatat
     3661 tccggcgacc atcattcccg gtgttgctgt accgctgtcg ttaattggca cctttgcggt
     3721 catggtgttt ctcgattttt caatcaataa cctgacgttg atggcgttaa ctatcgccac
     3781 cggattcgtg gtcgatgacg ccatcgtggt gatcgaaaac atttcccgct atatcgaaaa
     3841 aggcgaaaaa ccgttggcgg cggcgctcaa aggcgcgggt gaaatcggct ttaccattat
     3901 ctcgctgacc ttctcgctga ttgcggtgtt gatccccctg ctgtttatgg gcgatatcgt
     3961 cgggcgactg ttccgcgaat ttgctattac cctggcagtc gcgattttga tctcagcggt
     4021 ggtgtcgcta accctgacac cgatgatgtg cgcgcggatg ctcagccagg agtcgctgcg
     4081 taaacagaac cgcttctccc gtgcctcgga gaaaatgttc gacaggataa tcgccgccta
     4141 tggtcgtgga ctggcgaaag tgctgaatca tccgtggctg accttaagcg tggcgctcag
     4201 cacgctgctg cttagcgtgc tgctgtgggt gttcattccg aagggtttct tcccggtgca
     4261 ggacaacggc attattcagg gcactttgca ggcaccgcaa tccagctcct ttgccaatat
     4321 ggcccagcgg caacgccagg tcgcggacgt gattttgcag gatccggcag tgcaaagttt
     4381 gacctcattt gttggcgttg atggcactaa cccgtcgctg aacagtgcgc gtttacagat
     4441 caatctcaaa ccactggatg aacgagatga ccgggtgcaa aaagtcatcg cccgtctgca
     4501 aacggcggtg gataaagtgc cgggcgtcga tctcttcctg caaccaacgc aggacctgac
     4561 tattgatact caggtcagcc gcacccagta ccagtttacc ttgcaggcca cgtcactgga
     4621 tgcgctcagt acctgggtgc cacagttgat ggaaaaactc cagcagctgc cacagctttc
     4681 tgatgtctcc agcgactggc aggacaaagg gctggtggcg tatgtcaatg tcgacaggga
     4741 cagcgccagc cgtctgggga tcagcatggc ggatgtcgat aacgccctgt acaacgcgtt
     4801 tggtcagcga ctgatttcca ctatttatac tcaggctaac cagtatcgcg tggtgctgga
     4861 acacaacacc gaaaataccc caggcctcgc ggcgctggat accattcgcc tgaccagcag
     4921 cgacggtggc gtggtgccgt taagctcaat tgccaaaatt gagcagcgtt ttgcgccgct
     4981 ttccatcaac catctggatc agttcccggt aacgactatc tcctttaacg tgccggataa
     5041 ctattcgctg ggcgatgcgg tgcaagcgat tatggataca gagaagacgc tgaatctgcc
     5101 ggtggatatc accacgcagt tccagggcag caccctcgcc ttccagtcag cgctgggcag
     5161 cactgtctgg ctgattgtcg cggcggtggt ggcgatgtat atcgtgctcg gcattctgta
     5221 cgagagcttt attcacccga tcaccattct ctcgacgcta cccaccgcag gggtcggcgc
     5281 gctgctggcg ttaatgattg ccggtagcga actggatgtg attgccatta tcggcattat
     5341 tttgctgatc ggtatcgtga agaagaacgc catcatgatg atcgacttcg cgctggctgc
     5401 tgagcgcgag caaggcatgt cgccgcgcga agctatttac caggcttgtc tgttgcgttt
     5461 tcgtccgatc ctgatgacca ctctggcggc tctgcttggt gcgctgccgc tgatgttgag
     5521 taccggggtc ggcgcggaac tgcgtcgtcc gttaggtatc ggcatggtcg gcggtctgat
     5581 tgtcagccag gtgctgacgc tgtttaccac gccggtgatt tatctgctgt tcgaccgtct
     5641 ggcattgtgg accaaaagcc gctttgcccg tcatgaagag gaggcgtaag tgaagttttt
     5701 tgccctcttc atttaccgcc cggtggcgac gattttactg tcggtcgcca ttaccctgtg
     5761 cggcatactg ggtttccgta tgctgccagt cgccccgctg ccgcaggtcg attttccggt
     5821 gatcatggtc agcgcctcgc tgcccggtgc gtcaccagaa acaatggcgt cttccgttgc
     5881 cacgccgctg gagcgctcac ttgggcgcat tgccggagtc agcgaaatga cctccagcag
     5941 ttcgctcggc agcacgcgca ttattttgca gtttgatttt gaccgggata tcaacggcgc
     6001 agcgcgcgat gtgcaggcgg cgatcaacgc cgcgcaaagt ctgctaccca gcgggatgcc
     6061 cagccgtccg acctatcgca aagcgaaccc gtcggatgcg ccaattatga tcctcacgct
     6121 gacatccgat acttattcgc agggtgaact gtacgatttc gcctcgacac agctggctcc
     6181 gacgatttcg caaatcgacg gtgtcggtga tgtcgatgtt ggcggcagct cgctgcccgc
     6241 cgtacgcgtc gggctgaatc cgcaagcgct gtttaatcag ggcgtgtcgc tggacgatgt
     6301 gcgcaccgcc atcagcaatg ccaacgtgcg taaaccgcag ggggcgctgg aagatgatac
     6361 tcaccgctgg cagatccaga ctaacgatga actgaaaact gccgctgaat atcagccgct
     6421 gattattcat tacaacaacg gcggcgcggt tcgtctgggc gatgtggcga cggtgaccga
     6481 ctcagtacag gatgtacgta acgccgggat gaccaacgcc aaaccggcca ttttgctgat
     6541 gatccgcaag ctgccggaag ccaatattat ccagacggta gacagcatcc gcgcccgtct
     6601 gccggaatta caatccacca ttccggcggc gattgatctg caaattgccc aggatcgctc
     6661 ccctactatc cgcgcgtcgc tggaagaggt tgagcagacg ctggtaatct cagttgcact
     6721 ggtgattctg gtggtgtttt tgttcctgcg ttcgggtcgc gccaccatta ttcccgccgt
     6781 tgcggtgccg gtttcgctga ttggtacgtt tgcggcgatg tacctgtgtg gtttcagtct
     6841 caataatctt tcgttaatgg cgctcaccat cgccactggc tttgtggtgg atgacgccat
     6901 cgtggtgctg gaaaacattg cccgtcatct ggaagcgggg atgaaaccgc tacaggccgc
     6961 gctacaaggt actcgcgaag tcggctttac ggtgctgtcg atgagtctgt cactggtggc
     7021 ggtgttcctg ccgctgctgt tgatgggcgg attgccgggt cgcctgttac gcgagtttgc
     7081 cgtgacgctt tctgtcgcca ttggtatttc gttgctggtt tccctgacgt taacgccgat
     7141 gatgtgcggc tggatgctaa aagccagcaa gccgcgcgag caaaagcgac tgcgtggctt
     7201 tggtcgcgtg ttggtagccc tgcaacaagg ctatggcaag tcgctgaaat gggtgctcaa
     7261 tcatacccgt ctggtgggcg tggtgctgct tggcaccatt gcgctgaata tctggctgta
     7321 tatctcgatc ccgaaaacct tcttcccgga gcaggacacc ggcgtactga tgggcgggat
     7381 acaggctgac cagagtattt cgtttcaggc gatgcgcggt aagttgcagg acttcatgaa
     7441 aattatccgt gacgatccgg cggtggataa tgtcaccggc tttaccggtg gttcacgggt
     7501 gaacagtgga atgatgttta tcacccttaa accacgcggc gagcggagcg aaacggccca
     7561 acagattatc gatcgcttgc gtaaaaaact ggcgaaagaa ccgggagcga atctgttcct
     7621 gatggcggta caagatattc gcgttggcgg acgtcaggct aacgccagct accagtacac
     7681 attattgtcc gacgatctgg cggcactgcg tgaatgggag ccgaaaattc gcaaaaaact
     7741 ggcaacattg ccagagctgg ctgatgttaa ctccgatcag gaagataatg gcgcggagat
     7801 gaaccttatt tacgatcgcg acaccatggc gcgattggga attgacgtgc aagccgccaa
     7861 cagcctgtta aataacgcct tcggtcagcg gcaaatctcg accatttacc agccgatgaa
     7921 ccagtacaaa gtggtgatgg aagttgatcc gcgctatacc caggacatca gtgcactgga
     7981 aaaaatgttc gttatcaata acgaaggaaa agcgatcccg ctgtcgtatt tcgctaaatg
     8041 gcaaccggcg aacgctccac tgtcggtgaa tcatcaggga ttatcggcgg cctcgaccat
     8101 ttcgttcaac ctgccgaccg ggaaatcgct ctcggacgcc agtgcggcga tcgatcgcgc
     8161 aatgacccag cttggtgtgc cttcgacggt gcgcggcagt tttgccggaa cagcgcaagt
     8221 gttccaggag acgatgaact cacaggtgat cctgattatc gccgccatcg ccacggtata
     8281 tatcgtactg ggtatccttt acgagagtta tgtgcatcca ctgacgattc tctccaccct
     8341 accctcggcg ggcgtcggag cgctgttggc gctggagctg ttcaatgccc cgttcagcct
     8401 aattgcgctg atagggatca tgttattaat cggcatcgtg aagaaaaacg ccattatgat
     8461 ggtcgatttt gcgcttgaag cccaacggca cggtaacctg acgccgcagg aagctatttt
     8521 ccaggcctgt ctgctgcgtt ttcgcccgat tatgatgact accctggcgg cgctgtttgg
     8581 tgcgctaccg ctggtattgt cgggcggtga cggctcggag ttgcgccaac cattggggat
     8641 caccattgtc ggcggtctgg tcatgagcca gcttctgacg ctgtatacca cgccggtggt
     8701 gtatctcttt ttcgaccgtc tgcggctgcg tttttcgcgt aaacctaaac aagcggtaac
     8761 cgagtaaatg acagatcttc ccgacagcac ccgctggcaa ttgtggattg tggctttcgg
     8821 cttctttatg cagtcgctgg acaccaccat cgttaacacc gcgcttccct caatggcgca
     8881 aagcctcggg gaaagtccgt tgcatatgca catggtcatt gtctcttatg tactgaccgt
     8941 ggcggtgatg ctgcccgcca gcggctggct ggcggacaaa gtcggcgtgc gcaatatttt
     9001 ctttaccgcc atcgtgctgt ttactctcgg ttcactgttt tgcgcgcttt ccggcacgct
     9061 gaacgaactg ttgctggcac gcgcgttaca gggcgttggc ggcgcgatga tggtgccggt
     9121 cggcaggttg acggtgatga aaatcgtacc gcgcgagcaa tatatggcgg cgatgacctt
     9181 tgtcacgctg cccggtcagg tcgggccgct gcttggcccg gcgctcggcg gtctgctggt
     9241 ggagtacgca tcgtggcact ggatcttttt gatcaacatt ccggtgggga ttattggcgc
     9301 gatcgccaca ctgatgttaa tgccgaacta caccatgcag acgcggcgct ttgatctctc
     9361 cggctttctg ttgctggcgg ttggcatggc ggtattaacc ctggcgctgg acggcagtaa
     9421 aggtacaggt ttatcgcagc tggcgattgc tggcctggtc gcagttggcg tggtagcact
     9481 ggtgctttat ctgctgcacg cccaaaataa caaccgcgcc ctgttcagtc tgaaactgtt
     9541 ccgcactcgt accttttcgc tgggcctggc ggggagtttt gccggacgta ttggcagtgg
     9601 catgttgccc tttatgacac cggttttcct gcaaattggt ctcggtttct cgccgtttca
     9661 tgccggactg atgatgatcc cgatggtact cggcagcatg ggaatgaagc gaattgtggt
     9721 acaggtagtg aatcgctttg gttatcgtcg ggtactggtg gcgaccacgc tgggcctgtc
     9781 gctggtcacc ctgttgttta tgactaccgc tctgctgggc tggtactacg ttttgccgtt
     9841 cgtcctgttt ttacaaggaa tggtcaactc gacgcgtttc tcctccatga acaccctgac
     9901 gctgaaagat ctcccggaca acctggcgag cagcggcaac agtctgctgt cgatgattat
     9961 gcaattgtcg atgagtattg gcgtcaccat cgccgggctg ttgctgggac tttttggttc
    10021 gcagcatgtc agcgtcgaca gcggcaccac acaaaccgtc tttatgtaca cctggcttag
    10081 catggcgttt atcatcgccc ttccggcgtt tgtctttgcc agagtgccga gcgatacgca
    10141 tcaaaatgtc gctatttcac ggcggaaaag gagcgtgcaa tgaagttctg gcgacccggt
    10201 attaccggca aactgtttct ggcgattttc gctacctgca ttgtcttatt gatcacgatg
    10261 cactgggcgg tacgtatcag ttttgagcgc ggctttatcg attacatcaa gcatggtaat
    10321 gaacaacggc tgcaaatgct cggcgatgcg cttggtgagc agtacgccca gcacgggaac
    10381 tggcgcttcc tgcgtaataa cgatcgcttt gtatttcaga tcctacgttc actggagcat
    10441 gataacaacg aagataagcc cggccccggt atgccgccac acggctggcg cacacaattt
    10501 tgggtggttg atcaaaacaa caaagtgctg gttggcccgc gagcaccggt tccacccgac
    10561 ggcacacggc ggcccattat ggtcaatggt gcggaagttg gcgcggtgat cgcctcccct
    10621 gttgaacgac tgacccgtaa tactgatatc aattttgaca gacaacagcg gcaaaccagt
    10681 tggctgattg tggctttatc taccttatta gcggcgctgg cgacattccc actggcgcgc
    10741 ggtttgctgg ctccggtaaa acgactggtg gatggcacgc acaaactggc agcgggtgat
    10801 ttcactactc gcgtagcccc caccagcgaa gatgagttgg gcagactggc agaggatttc
    10861 aatcaactcg ccagcacact ggagaaaaac cagcagatgc gccgcgattt tatggccgat
    10921 atttctcatg aactgcgcac gcctttagca gtactgcgcg gcgaactgga agccattcag
    10981 gatggtgtgc gtaaattcac gccggagacg ctggcttctt tacaggcaga agtcggtaca
    11041 ctgacaaaac tggttgacga tctccatcaa ttgtctatgt ctgatgaagg cgctctcgcc
    11101 tatcaaaaag caccggtaga tttgatccca ctgctggaag tggcgggagg cgcatttcgc
    11161 gaacgattcg ccagccgtgg cctgaaattg caattttccc tgccagacag tattaccgta
    11221 tttggcgatc gcgatcgttt aatgcagttg ttcaataact tactggaaaa tagcctgcgc
    11281 tacactgaca gcggcggcag cctgaaaatc tctgccgagc agcacgacaa aacggtgcgc
    11341 ctgacctttg ccgacagtgc gccgggtgtc agtgacgatc agctacaaaa attgtttgaa
    11401 cgtttttatc gcaccgaagg ttcccgcaac cgcgccagcg gtggttccgg gctggggctg
    11461 gcgatttgcc tgaacattgt tgaagcacat aacggccgca ttattgccgc ccattcgcct
    11521 tttggcgggc ttagcattac agtagagtta ccgctggaac gggatttaca gagagaagta
    11581 tgaccgagtt accaatcgac gaaaacacac cgcgtatttt gatcgtggaa gatgaaccga
    11641 agctggggca gttgctcatt gattatctgc gtgctgcgag ctatgcgcca acgttgatta
    11701 gccacggcga tcaggtactg ccatatgtgc gccagacacc accggatctg atactgttgg
    11761 atctgatgct ccctggcacc gatggcctga cgctgtgtcg ggagattcgt cgtttttctg
    11821 acattccgat cgtgatggtg acagcaaaaa tcgaagagat cgaccgcctg ctcggactgg
    11881 agattggcgc agatgattat atctgcaagc cgtacagccc acgggaagtg gtagcgcgcg
    11941 tcaaaaccat tttgcgccgt tgcaaaccgc agcgcgagtt gcagcaacag gatgctgaaa
    12001 gcccgttgat tatcgacgaa ggtcgttttc aggcttcctg gcgcggtaaa atgcttgacc
    12061 tgacgcctgc agaatttcgt ctgctgaaaa cgctctctca tgaaccagga aaagtgttct
    12121 cccgcgagca gttgctgaac catctttatg acgactaccg cgtggtaacc gaccgtacca
    12181 tcgacagcca cattaaaaac ctgcgccgca agctggaatc tctcgacgcc gaacagtcat
    12241 ttatccgcgc cgtttacggc gtcggttacc gctgggaagc cgacgcctgc cgcatcgttt
    12301 aacattttta cctgccggag atggccttca gaccagatcc ggcaacatta tcccacgcat
    12361 ggtcagcaaa ctgacattat ctctccgccc tggcaggcac cagaaaatcg ctcatacttt
    12421 aattggcaaa cagtaccttt agatgctgtt ttcgatacgc aatttcaatc aaggagtcat
    12481 tatggctggt tggtttgaac tcagtaagag cagtgataat cagttccggt ttgtgctaaa
    12541 agcgggcaat ggtgagacta tcctcaccag cgagctgtat acctcaaaag cctctgcgga
    12601 aaagggcatc gcgtcggtgc gtagcaacag cccgcaagaa gaacgctatg agaaaaaaac
    12661 ggcaagcaat ggcaaattct atttcaatct gaaagccgcc aatcatcaaa ttatcggctc
    12721 cagccagatg tatgccaccg cgcaatctcg tgaaaccgga atagcctccg ttaaagtaaa
    12781 tggcacaagc cagacggtga aagacaatac gtaattacga tgccgggcgt aggttgttgg
    12841 cgtccggcgc tttaccagtt acccccacag cgctacaatg cccgccctta aagtgggggc
    12901 actcccctaa ccgcttcatc aggtgaagcg gctctgacct gtcatcagaa cgagagaatt
    12961 atgtttaaac cggaactcct ttccccggcg ggaacgctga aaaatatgcg ttacgctttc
    13021 gcttatggcg cagatgctgt ttatgcgggc cagccgcgtt actccctgcg tgtacgcaac
    13081 aacgaattca accacgaaaa tcttcagctc ggcatcaatg aagcccacgc gctggggaaa
    13141 aagttttatg tcgtggtcaa cattgcaccg cacaacgcca agctgaaaac ctttatccgt
    13201 gacctgaaac cggtggtgga aatggggccg gatgcgctga ttatgtccga tccagggctg
    13261 attatgctgg tgcgtgagca cttcccggaa atgccaatcc acctctcggt acaggctaac
    13321 gccgtaaact gggcgacggt gaaattctgg cagcaaatgg gtctgacccg cgtgatcctc
    13381 tctcgcgaac tgtcactgga agagattgaa gagatccgca atcaggtgcc ggatatggag
    13441 atcgaaatct tcgttcacgg cgcgctgtgc atggcctact ccggtcgctg cctgctctct
    13501 ggctatatca acaagcgcga cccgaaccag ggcacctgca ccaacgcctg ccgctgggag
    13561 tataacgtcc aggaagggaa agaagatgac gtcggcaaca tcgtacacaa gtacgagccg
    13621 attccggtgc aaaatgttga gccgacgctg ggtatcggcg cgccaaccga caaagtgttt
    13681 atgatcgaag aagcccagcg tccgggcgag tatatgaccg cgtttgaaga tgagcacggc
    13741 acttacatca tgaactcgaa agatctgcgc gccatcgccc atgttgaacg cctgaccaaa
    13801 atgggcgtgc attcgctgaa aatcgaaggc cgtaccaaat ctttctacta ttgcgcacgc
    13861 accgcgcagg tttaccgtaa agctatcgat gacgccgctg cgggcaaacc gttcgatacc
    13921 agcctgctgg aaacgctgga aggtctggcg catcgtggct ataccgaagg tttcctgcgt
    13981 cgtcataccc acgacgatta tcagaactac gaatacggtt attcggtttc tgaccgccag
    14041 cagtttgttg gtgagtttac cggtgagcgc aagggggagc tcgcggcggt agcggtgaaa
    14101 aataaattct ccgttggcga cagccttgag ctaatgacgc cgcaaggcaa cattaacttt
    14161 acccttgagc atatggaaaa cgccaaaggt gaagcaatgc cggtcgcacc aggcgatggt
    14221 tatactgtgt ggctcccggt gccgcaggat cttgagctaa attacgcgct gctgatgcgt
    14281 aatttctccg gggaaaccac gcgtaacccc cacggtaagt gattaatttc gattattttt
    14341 cccggatgga aaattcttag aaaccgatca catacagctg catttattac ggttatcatc
    14401 cgtttcgctg aaaaacataa cccataaaat gctagctgta ccaggaacca cctccttagc
    14461 ctgtgtaatc tcccttacac gggcttattt tttatgcata agccctatcc ctggtcaccg
    14521 tcttccattg accacatcga tagaatcctc cttcatagca cgatgccttt cacttatcga
    14581 catcgtgctc gcacaggttc cggttacgca cagccagaac gcgcatgttt gacgcttacc
    14641 aaaaaatgaa cctaaagcat tggaatattt ttgacatcat tttctgatgg ctgcataaaa
    14701 taaaaattct gctttagttt catctatctg tttgtcatta ttactcacat tcaataatgg
    14761 tgttgaagaa tatcccatca cagataaaat aaatatgtgc atggtagtct tgaatctatt
    14821 ctcactctcc acatttgaat gtcagacgag cgacgccatg taatcctgca ccttctgtct
    14881 tcaggtcaac tatctgcatt tttttgccct gggtaacaca gaaatgagtt gcatcatttt
    14941 ttactatatt ttctgcacca gatattctac ccctggctaa agaagcttcg gcttcggtgt
    15001 agtattggtt atcgagttta cgctgaatat tacttttata tgcaaggcca aatttaccga
    15061 tacttgtctc atcattatgc acagcacaac cagacataag aaaaacacta attaatgata
    15121 tagcagctat ctttttcac
//
LOCUS       DAZFJL010000115        15103 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFJL010000115 DAZFJL010000000
VERSION     DAZFJL010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.115"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(66..539)
                     /locus_tag="ACHJ8C_003515"
     CDS             complement(66..539)
                     /locus_tag="ACHJ8C_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO5553306.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHTYTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(641..1489)
                     /gene="yiaJ"
                     /locus_tag="ACHJ8C_003516"
     CDS             complement(641..1489)
                     /gene="yiaJ"
                     /locus_tag="ACHJ8C_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFO5553307.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            1690..2688
                     /gene="yiaK"
                     /locus_tag="ACHJ8C_003517"
     CDS             1690..2688
                     /gene="yiaK"
                     /locus_tag="ACHJ8C_003517"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFO5553308.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNHRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTEDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTSAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            2700..3164
                     /locus_tag="ACHJ8C_003518"
     CDS             2700..3164
                     /locus_tag="ACHJ8C_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO5553309.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRTIDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPACNKNATTAIRKIVVKVALAAL"
     gene            3187..4107
                     /locus_tag="ACHJ8C_003519"
     CDS             3187..4107
                     /locus_tag="ACHJ8C_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4862 family protein"
                     /protein_id="HFO5553310.1"
                     /translation="MKTNNAGYIIGAYPCAPSFHQRSEEEEKDFWRQLSETPDILGLE
                     QPCLENFHPLGDQWLLRHTPESWKFVVTAVMETMRRRSENSGFGLASSDEEQRQACVA
                     YYRHLFNKINSLQANKVLALELQAAPLATNPNVMQATDAFARSLKEIASWDWPCKLVL
                     EHCDAMTSSSPRKGFLPLENVLEVITDYDISICINWARSAIEGRNTTLPLTHTQMAKQ
                     AGKLGALMFSGTTLNGAYGEWQDLHAPFAPFCAESLMTTDHVRELFNVAESSTLHFAG
                     IKLLEINATADVHHRIEILRNGIHSLNESR"
     gene            4221..4688
                     /gene="yiaM"
                     /locus_tag="ACHJ8C_003520"
     CDS             4221..4688
                     /gene="yiaM"
                     /locus_tag="ACHJ8C_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFO5553311.1"
                     /translation="MKRILEGILAIIIAILSCIIFINIILRYGFHTSILSIDELSRLL
                     FVWLTFIGAIVAYMDNSHVQVTFLVEKLSPANQQRVSLLTHTLILLLCLGLAWGAIEK
                     TAQDWSNLSPILGVPVGLMYAAAIPTSLIIALLELRHLYRQFTNTPSRNQQGA"
     gene            4691..5968
                     /gene="yiaN"
                     /locus_tag="ACHJ8C_003521"
     CDS             4691..5968
                     /gene="yiaN"
                     /locus_tag="ACHJ8C_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFO5553312.1"
                     /translation="MTILIFIVSLLGAIAIGVPIAWALLLCGISLMFWMDIFDVQILA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSQRIVDLPMKLVGHRPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRQANYPVNRAAGLIGSGGIIAAIIPPSIPLIIFG
                     VSSGLSISKLFMAGIAPGIMMGVTLMVTWWWQAKRLNLPCQPKASLREVWQSLVSGIW
                     ALFLPIIIIGGFRSGLFTPTEAGAVAAFYALFVSVVVYREMTFSTLYHVLINAAKTTS
                     VVMFLVASAAVSAWLITIAELPMMVSELLQPLVDSPRLLFIVIMLAIMVISTVMDLTP
                     TVLILTPVLMPLVKEAGIDPVYFGIMFIINCSISLITPPVGNVLNVVCGVAKLKFDDA
                     VKGVAPYVMVLFMLLALFIFIPELITAPLKWMS"
     gene            5981..6967
                     /gene="yiaO"
                     /locus_tag="ACHJ8C_003522"
     CDS             5981..6967
                     /gene="yiaO"
                     /locus_tag="ACHJ8C_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFO5553313.1"
                     /translation="MKLRSVTYALLIAGVATFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNELLQEKTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFTGLSPVIN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKVSLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FYEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGSYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            6971..8467
                     /gene="lyxK"
                     /locus_tag="ACHJ8C_003523"
     CDS             6971..8467
                     /gene="lyxK"
                     /locus_tag="ACHJ8C_003523"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFO5553314.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCTAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSRGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGLEDEFTLNAVMGTWAVTSGITHGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFAEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGIYHNFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGYHARIKEHTL"
     gene            8464..9126
                     /gene="ulaD"
                     /locus_tag="ACHJ8C_003524"
     CDS             8464..9126
                     /gene="ulaD"
                     /locus_tag="ACHJ8C_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO5553315.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTRLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKEIRVKAFIAGRALAGAANPAQVAADFHAQIDSIWGGK
                     RA"
     gene            9119..9979
                     /locus_tag="ACHJ8C_003525"
     CDS             9119..9979
                     /locus_tag="ACHJ8C_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFO5553316.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMIETGVAIPSMCLSAHRRFPFGSRDDVVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTQQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     HSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            9973..10668
                     /gene="araD"
                     /locus_tag="ACHJ8C_003526"
     CDS             9973..10668
                     /gene="araD"
                     /locus_tag="ACHJ8C_003526"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO5553317.1"
                     /translation="MLEQLKADVLAANLALPVHHLVTFTWGNVSAMDETRQWMVIKPS
                     GVEYEVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRFAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRLMTAEEINGEYEYQTGEVIIKTFEERGLNP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(10715..12253)
                     /gene="aldB"
                     /locus_tag="ACHJ8C_003527"
     CDS             complement(10715..12253)
                     /gene="aldB"
                     /locus_tag="ACHJ8C_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO5553318.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(12361..13656)
                     /locus_tag="ACHJ8C_003528"
     CDS             complement(12361..13656)
                     /locus_tag="ACHJ8C_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFO5553319.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHTAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISVS
                     LSVSDNTARTDLRTIVKEGFAQEKRINNQQAVYVAHYPL"
     gene            complement(13786..14937)
                     /gene="yiaY"
                     /locus_tag="ACHJ8C_003529"
     CDS             complement(13786..14937)
                     /gene="yiaY"
                     /locus_tag="ACHJ8C_003529"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO5553320.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDVEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         3642 a         3961 c         3838 g         3662 t
ORIGIN      
        1 ttttagccgc atccggcata aacaaagcgc actatccctg cgttgagaaa cgccggaaaa
       61 acgtttcaaa caggctgctg ccgtagccga tgccgctgca ctttctcgac atccatgcac
      121 tgcaacgcct ttgtcgggca ggcttcaaca caggccggcc ccgtcgcccg atgccagcac
      181 agatcgcact taatcgccct cgcctttttg cgcgaagaaa cgacctccat cgcaccaaac
      241 gggcaagcca gcatacagct tttacagcca atgcaacgtg attgttcaac gaaaatatgc
      301 ccatgttcgc ggcttatcgc gtcaacaggg cacacatttg cgcacggtgc atcttcacac
      361 tgatgacagg ctactgccgt ggtgtaagta tgatctttaa tgacacgaat acgggaaata
      421 aactcgtctg gtgacaacgc agcgcaatcc tgattctcct gatgcgacac tgcgcaagcc
      481 acttcacagg tacggcaacc gatacatttc gtcgcatccg caataataaa ccggttcatg
      541 atcactcctt tgcactaaaa cagcagagtg ccagagagcg cgtccctact gctttgatcc
      601 aacgaaggaa atgttaaagt tttggcactt gtccaaaggg ttatgtaatc gcgccctggt
      661 cgtccctgac agtaaatccc agttcattag aaatagcctg cgcggtttca cgaagtggtt
      721 tcaggagatt tttctctccc acctgtttca gacgtgatgt cgaaagcgaa atcgacacgg
      781 cgtacggcac ccgcccatga atatcaaaca ccggaacagc aatacaggag acgccgagtt
      841 cgttttcttc tctgtccatc gccgctccac tttcacgaat gtgcgccagt tcgtcgaaca
      901 tcgcgggcag ctcggtaatg gtattgcggg ttaacggctg gatctcatgc tgatggcttt
      961 cccagtatga cttcacgtag tccgggtgac caaacgccat gtagatcttg cccattgcgg
     1021 aacagtagag cggcatatgc tggcctatat aggcacgggt tcgcagcatc ccggttgtgg
     1081 gttccagctt ataaatcaaa atagcgtgat cgtcttcgcg gctggagaag ttaatagttt
     1141 caccagtggc gatgttcaga gcctcaagat gcggagcggc gatatgaatg atattcagcg
     1201 aagatagcgc cttctgcccg acggcaataa atttggtggt caggcgataa ctccctgcag
     1261 cgggcgcggt ggtcacatag ccgcaggact gtaatccctg caataagcga tggacggtac
     1321 tcttatttaa accagccagc tccgaaagat gcgccaacgg acaaccgttt ggatagttgc
     1381 tcaaaatctc aatcagcatc aacccacgaa acagactctg gcttccggct gggcgctctt
     1441 tttcctgcgc catctcgttc tctttttttc ccatcacttc tttccccatt ttgtcgcgtc
     1501 ctgatggtag cgcaaagtgt gccgtagttc acgatctcga cagataattt ataaccactt
     1561 gatttttaag ttttttgaaa ttcatcaatc agattgcttt gttaaaaagt gatcgatata
     1621 tttgaaatca agtttcgcat attgaaattt taagccaaaa aagcgatcaa aaaaacaagg
     1681 aagcctggga tgaaagtgac atttgagcag ttaaaagcag cctttaatcg ggtcttaatt
     1741 tcacgcggcg ttgacagcga aacggctgac gcctgtgcag agatgttcgc ccgcaccacc
     1801 gaatccggcg tttattctca cggcgttaat cgtttccctc gtttcattca acaactggaa
     1861 aacggcgata tcattcctga tgcccaaccc aaacgtataa ccagcctcgg cgcaattgaa
     1921 cagtgggacg cccagcgttc gatcggtaac ctgacagcga aaaagatgat ggatcgcgcc
     1981 attgaactgg ctgccgatca cggtattggt ctggtggcac tacgtaatgc caaccactgg
     2041 atgcgcggcg gcagctacgg ctggcaggcg gcggaaaaag gctatattgg catttgctgg
     2101 accaactcca tcgccgtaat gccaccgtgg ggcgcaaaag agtgtcgcat cggcaccaac
     2161 ccgctgatcg tcgccattcc ttccacgccg atcaccatgg tcgatatgtc gatgtcgatg
     2221 ttctcttacg gcatgttaga agttaatcgc ctggctggtc gtcagctccc ggtagatggt
     2281 ggctttgatg atgagggcaa tttgaccaaa gaacctggcg ttatcgagaa aaatcaccgc
     2341 attttgccga tgggctactg gaaaggttcg ggcatgtcga ttgtgctgga tatgatcgct
     2401 accctccttt ctgacggcgc atcggttgcc gaagtcaccg aggacaacag cgacgaatac
     2461 ggcatttcac aaatttttat tgccattgaa gtggacaagc ttatcgacgg tcccacccgc
     2521 gatgccaaac tgcaacgcat catggattac gttaccagtg ccgagcgtgc tgacgaaaat
     2581 caggctattc gtttacccgg ccacgaattt actaccctgc tggcagaaaa ccgccgtaac
     2641 ggtattaccg ttgatgacag cgtgtgggca aaaatccagg cgttataagg agataagcca
     2701 tgatttttgg acatatcgcg cagcccaatc cgtgccgttt gcccgccgcc attgaaaagg
     2761 cgctcgattt tctgcgcacc atcgacttca acgccctgga accgggcgtt gtcgaaatcg
     2821 acggcaagaa tatttatgcg caaattatcg atttaaccac acgcgaagcg gtagaaaacc
     2881 gtccggaagt gcatcgtcga tatatcgata ttcagtttct ggcgtggggt gaagagaaaa
     2941 tcggcattgc tattgatacg ggaaataata aagtcagcga atcactatta gagcagcgcg
     3001 atattatttt ttatcacgat agtgaacatg aatcatttat agaaatgatt ccgggcagct
     3061 acgctatttt cttcccgcag gatgttcatc gcccggcatg taataaaaat gccactacgg
     3121 caatacgcaa gatcgtggtt aaagtggccc tcgctgcttt ataaaaaatg attactggag
     3181 cacataatga aaacgaataa tgccggttat attatcggcg cgtatccctg tgcaccttct
     3241 tttcaccaaa gaagtgaaga ggaagagaag gatttctggc gacaattatc tgagactcca
     3301 gacattttgg ggctggaaca accttgcctg gaaaattttc acccgctggg cgatcaatgg
     3361 ttactccgtc atacgccaga gagctggaaa tttgtcgtca ccgcagtgat ggaaaccatg
     3421 cgtcgccgca gcgaaaacag cgggtttggt ctcgcctcca gtgatgaaga acagcgccag
     3481 gcctgcgtgg cgtactatcg ccacctgttc aataaaatca actcattgca ggctaataaa
     3541 gtgctcgcac tggagctcca ggctgcaccg ttagcaacga atcctaatgt tatgcaagcg
     3601 acggacgctt ttgcccgctc attgaaagaa atcgccagtt gggactggcc gtgcaagctg
     3661 gtgctggaac actgcgacgc catgacatct tcttcccccc gaaaaggctt tttgccttta
     3721 gaaaatgtgc tggaagttat taccgattat gacatcagca tctgtatcaa ctgggcgcgc
     3781 tctgccattg aagggcgcaa tacaacgctg ccgctgaccc acacccaaat ggcgaaacaa
     3841 gccggaaaac tcggcgcgtt gatgttctct ggtacgacgc taaacggggc atacggcgaa
     3901 tggcaggatt tacacgcacc gtttgcacca ttctgtgcag aaagcctgat gaccacagat
     3961 catgttcgcg agttatttaa tgtagcagaa tcgtcaacat tgcattttgc tggcatcaag
     4021 ctactggaaa taaatgcgac ggctgatgtg caccatcgca tcgaaatatt acgtaacggc
     4081 attcactctc tgaatgaatc tcgttaaata gttccgctta tatatcactt tgaatatcta
     4141 attagccgtt ttctgataat tattacgaat attaacaaca ttagcgcata aaaaatattg
     4201 cataaccaga aggactcgtt atgaaacgga tactcgaagg cattcttgcg atcatcatcg
     4261 caatactgtc ttgtattatt tttattaata tcattcttcg atatggattc cacacaagta
     4321 tattatcgat tgatgaatta tcccgacttt tatttgtctg gttaaccttt attggcgcaa
     4381 tcgttgctta tatggacaac agccatgtgc aggttacttt tttagtcgaa aaactctcac
     4441 cggctaatca acaacgtgtt tcgctgctaa cccatacgct cattttattg ctgtgtctgg
     4501 ggctggcctg gggcgctata gaaaaaacag cgcaagactg gagcaactta tcgcccattc
     4561 ttggcgtccc cgttggctta atgtacgccg ccgctatccc caccagtctg attatcgcat
     4621 tgctggaatt gcgtcactta taccggcagt tcacaaatac ccccagccga aaccaacaag
     4681 gagcctgacg atgacaatcc ttatatttat tgtcagtttg ttaggggcta tcgcaattgg
     4741 cgttcccatc gcctgggcat tattactgtg cggcatttca ttgatgttct ggatggatat
     4801 ctttgatgtg caaattctgg cacaaacgtt ggtaaatggc gcagacagct tctccctgtt
     4861 agccatcccc ttctttgtac tggcgggtga gatcatgaac gcgggcggcc tgtcgcaacg
     4921 tatcgtcgac ttgccaatga aactggtagg ccacaggcca ggcggtctgg gttacgtggg
     4981 cgtgctggca gcgatgatca tggcaagcct gtccggttct gcggttgctg ataccgcagc
     5041 tgtcgcggcg ctgctggtgc cgatgatgcg ccaggccaac tatccggtaa accgcgccgc
     5101 cgggcttatc ggttccggcg ggattattgc ggccattatc ccgccttcca taccactcat
     5161 tatcttcggc gtctccagcg gactctccat cagcaaactg tttatggcgg gcattgcgcc
     5221 aggcatcatg atgggcgtca cgctgatggt tacctggtgg tggcaggcaa aacgactgaa
     5281 tttaccttgt cagccgaaag cctcactgcg agaagtctgg caatccttag tttccggtat
     5341 ttgggcgctc tttttaccga taattattat tggcggtttc cgttcggggt tatttacacc
     5401 gaccgaagca ggcgcggttg ccgccttcta cgctctgttc gtctcggtgg tggtctatcg
     5461 cgagatgacc ttctccacgc tttatcatgt gctgattaac gcggcaaaaa ccacatccgt
     5521 ggtgatgttc ctggtggcct ctgctgcggt atctgcatgg cttattacga tcgccgaatt
     5581 acccatgatg gtatcggagc tattacaacc gttggttgat tcgcctcgct tattattcat
     5641 cgtcattatg ctcgctatta tggtgatcag tacggtcatg gacttaacgc ctaccgtatt
     5701 aattcttaca ccagtattaa tgccattagt gaaagaagcg ggaatagatc cggtctattt
     5761 cggcatcatg tttattatca actgttcaat atcactgatt acgcctccag tcggtaatgt
     5821 tcttaacgtg gtatgcggcg tggcgaaact taaatttgac gatgcggtaa aaggcgttgc
     5881 gccttacgtc atggttttat tcatgttgtt agcattattt atttttatac cggagctcat
     5941 taccgctcct cttaaatgga tgagttaaaa ggaaatcata atgaaattac gttctgtaac
     6001 ctacgcttta ttaattgccg gagtggcgac gttcagcaca tcttctctgg cggcacaatc
     6061 attacgtttc ggttatgaaa cctcacaaac cgattcacaa catattgcgg cgaaaaaatt
     6121 taatgaatta ctgcaggaaa aaaccaaagg cgaactgaaa ttaaaactgt tcccggatag
     6181 cacccttggt aatgcgcagg cgatgatcag cggcgtgcgt ggcggcacta ttgatatgga
     6241 gatgtcaggc tctaacaact ttacgggact ctcgcccgtc atcaacctgc tcgatgtccc
     6301 gttcctgttc cgcgataccg ctcacgcgca taaaacgctc gacggcaaag tcggtgatga
     6361 cctgaaagtc tcccttgaag gtaaggggct gaaagtactg gcctactggg aaaacggctg
     6421 gcgcgatgtc accaactcgc gtgcaccggt caaaacgcct gccgacctga aagggttgaa
     6481 aatccgcacc aacaacagcc cgatgaatat cgctgcattt aaagtttttg gcgctaaccc
     6541 gatcccgatg ccatttgccg aagtctatac cgggctggaa acccgcacta tcgacgctca
     6601 ggaacacccg atcaacgtcg tctggtcagc aaaattttat gaagtgcaga agtacctttc
     6661 cctgacccat cacgcctatt ccccgcttct ggtggtgatc aacaaagcga agtttgatgg
     6721 cttaacgcct gagttccagc aggcactgat ctcatctgcg caggaagcgg gtagctatca
     6781 gcgcaaactg gtcgccgaag atcagcaaaa aatcatcgac ggtatgaaag aagcgggcgt
     6841 ggaagtcatc accgatctcg accgcaaagc ctttagcgat gcgctgggta cccaggtccg
     6901 tgacatgttt gttaaagatg ttccccaggg tgctgatctg ctgaaagccg tggatgaggt
     6961 gcaataaacc atgacgcaat actggctggg gttagattgt ggcggtagct ggctgaaagc
     7021 cgggctgtat gaccgagaag gccgggaggc gggcgtgcag cgcctgccgc tgtgcgcatt
     7081 aagtccacag ccaggctggg cagagcgcga catggcagaa ctgtggcaat gttgcactgc
     7141 tgtcattcgc gccctgctta cccattctgg cgtcagcggg gaacaaattg tcggtatcgg
     7201 catctccgcg cagggaaagg gcttatttct acttgataaa aacgacaaac cgctcggtaa
     7261 cgccattttg tcctcggatc gccgggcgat ggaaatcgtt cgtcgctggc aggaagatgg
     7321 catcccggaa aaactctatc cgctgacccg acaaacctta tggaccgggc atccggtgtc
     7381 gctgttacgc tggctgaaag agcacgaacc agaacgctac gcgcaaattg gctgcgtgat
     7441 gatgacgcac gactacctgc gctggtgttt aaccggcgtc aaaggttgcg aagagagcaa
     7501 tatttctgag tccaatctct acaacatgag tcgcggggaa tatgacccgt gccttaccga
     7561 ctggctgggg atcgctgaaa tcaaccacgc cctgccgcct gttgtcggat ctgccgaaat
     7621 ctgcggggaa atcaccgctc agacagccgt actgaccggt ctgaaagcgg gtacccccgt
     7681 tgtcggcggc ctgtttgatg tggtttccac cgcactctgt gccgggctcg aagacgaatt
     7741 caccctcaat gcggtgatgg ggacctgggc ggtgaccagc ggcataaccc acggtttacg
     7801 tgacggtgaa gcgcatccgt atgtctatgg tcgctacgtt aacgatggtc aatttatcgt
     7861 tcacgaagcc agccccacct cttccggcaa tctcgaatgg tttaccgcac agtggggaga
     7921 aatctcattt gctgagatta atcaggccgt tgccagtttg ccgaaagccg gtggcgatct
     7981 ctttttcctg ccgttcctgt acggcagcaa tgccggactc gagatgacca gtggtttcta
     8041 cgggatgcag gccattcaca cccgtgcaca cctcttacag gccatctatg aaggggtggt
     8101 gttcagccat atgacccatc tcaaccggat gcgcgaacgt tttactgatg tgcacaccct
     8161 gcgcgtcact ggcggcccgg cgcactccga tgtctggatg caaatgctgg cggacgtcag
     8221 cggtctgcgt atcgagctgc cgcaggtgga agaaaccggc tgttttggcg cggcacttgc
     8281 cgcccgcgtc ggcaccggga tttatcacaa cttcagcgaa gcccaacgtg acttacagca
     8341 cccggtacgc accctgctgc cggatatgac cgcccatcag ctttaccaac aaaaatacca
     8401 acgctatcaa catctcattg ccgcacttca gggttatcac gcccgcatca aggagcacac
     8461 attatgagtc gaccacttct gcaactggcc ctcgaccact catcacttga agccgcgcag
     8521 cgcgacgtga cgcggttaaa agacagcgtc gatattgtcg aagcaggcac cattctctgt
     8581 ttaaacgaag ggcttggcgc agtgaaagcc ttgcgcgaac agtgccctga caaaatcatc
     8641 gttgccgact ggaaggtcgc cgacgcgggt gaaacgctcg cgcaacaggc atttggcgca
     8701 ggcgctaact ggatgaccat catctgcgcc gcgccgctcg ccacggtaga aaaaggccac
     8761 gcgatggcgc aacgctgcgg cggtgaaatc cagatcgagc tgttcggcaa ctggacgctg
     8821 gacgacgccc gcgactggca tcgtattggc gtacgacagg cgatttatca tcgtggtcgt
     8881 gacgcgcagg ccagcgggca acagtggggc gaggccgatc tggcacgcat gaaggcgctt
     8941 tccgatatcg gccttgagct ttccattact ggcgggatta cccctgctga cctgccgctg
     9001 tttaaagaga tccgcgtgaa agcgtttatt gccggacggg cgctggcagg cgcagcaaat
     9061 ccggcgcaag tggccgctga ttttcatgcg caaatcgact ccatctgggg aggcaagcgt
     9121 gcgtaatcat ccgttaggca tttatgaaaa agcgctggcg aaagatctct cctggccgga
     9181 gcggctggtg ctggcaaaaa gctgtggttt tgattttgtc gaaatgtcgg tggatgaaac
     9241 cgacgaacgg ttgtcacgtc ttgactggag cgccgcacaa cgtgcttcgc tggtcactgc
     9301 gatgatcgaa acgggtgttg ctattccgtc aatgtgcctg tccgcacatc gtcgttttcc
     9361 ttttggtagc cgggacgacg tcgtgcgcca gcgggcgcgg gagattatga gtaaagcgat
     9421 tcgcctggcg cgcgatctcg gcattcgtac tattcaactg gcaggttacg acgtctatta
     9481 cgaggatcat gacgaaggca cccagcaacg gtttgctgaa gggctggcgt gggcggttga
     9541 acaggcagcg gcatcacaag tcatgctggc ggtggagatt atggacaccg cgtttatgaa
     9601 ctctatcagc aaatggaaaa agtgggacga aatgcttgcg tcaccgtggt tcaccgttta
     9661 cccggacgta ggcaacctca gcgcctgggg caatgatgtt cccgccgaac tgaaactggg
     9721 catcgaccgt atcgccgcca tccacctgaa agacactcaa cccgttaccg aacatagccc
     9781 cggacagttc cgcgatgtgc cgtttggcga aggctgtgtc gattttgttg gcatcttcaa
     9841 aacgctgcat gagctgaact atcgcggatc atttctgatt gaaatgtgga ccgaaaaagc
     9901 caaagagccg gtgctggaga ttattcaggc gcggcgctgg attgaagcgc gtatgcagga
     9961 ggccggattt atatgttaga gcaactgaaa gctgacgtgc tggcggcaaa cctggcgctt
    10021 cccgttcatc atctggtgac gttcacctgg ggcaatgtca gcgcgatgga cgaaacgcgg
    10081 caatggatgg tgattaagcc ttccggcgtt gagtatgagg taatgaccgc cgacgatatg
    10141 gtggtggttg agatagccag cggcaaggtg gtggaaggca gcaaaaagcc ctcttccgac
    10201 acgccaacgc atctggcgct ttaccgtcgc tttgcggaga ttggcggcat agtgcatacc
    10261 cactcgcgcc acgccaccat ctggtcgcag gccggacttg atctccccgc ctggggcact
    10321 actcacgccg attattttta cggagccatc ccctgcacgc gcctgatgac cgcagaagag
    10381 atcaacggcg agtatgaata ccagaccggc gaagtgatca ttaaaacctt cgaagagcgt
    10441 gggttgaacc cggcacaaat ccctgcggtg ctggtgcatt ctcacggccc gttcgcctgg
    10501 ggaaaaaatg ccgccgatgc ggtgcataac gccgtggtac tggaagagtg cgcctatatg
    10561 ggtctgttct cgcgccagct tgcgccacag ctccctgcga tgcaaaacga actgctggat
    10621 aagcactacc tgcgtaagca tggggccaat gcctattacg ggcagtagcc atacctgata
    10681 gtgcccggca atacacgccg ggcagccttt cacatcagaa cagccccaac ggtttatccg
    10741 agtagctcac cagcaggcac ttggtttgct ggtaatgctc aagcatcatt ttgtgggttt
    10801 cacggccaat gcctgattgt ttgtagccgc caaacgccgc atgtgccggg taagcgtgat
    10861 aacagttggt ccacacgcgc cctgcctgaa tgccgcgccc catcttatag gccagattac
    10921 cgttgcggct ccagacgccc gcgcccagac catattgcgt atcgttcgcc agctccagcg
    10981 cttcttccat cgttttgaag gtggttaccg ccagcaccgg gccaaaaatc tcctcctgga
    11041 acacgcgcat attgttctga ccaaacagaa tcgtcggttc gaggtagtag ccgtctttca
    11101 gttcgccttc cagcagctta cgtcgcccgc cggtgagcac atcagcgccc tcttttttac
    11161 cgatatcaat gtagttgagg atggtttcca gttgcccgtg agaaacctgc gcgcccattt
    11221 gcgtcacgct gtcgagcggg ttaccgctgc gaatgctttc gacacggcgg atagcgcgtt
    11281 ccataaagcg ttcgtagata gactcctgca ctaaagcacg gcttggacag gtgcaaactt
    11341 cgccctggtt aaaggcaaac agtgcaaagc cttccagcgc tttatcgaaa aaggcatctt
    11401 cttcatccat cacatcagca aagaagatat ttggcgattt gccgcccagc tccagcgtca
    11461 ccggaataat gttttgcgtg gcgtattgca taatttgttg gcccacttcc gttgagccgg
    11521 taaacgccac tttggcgatg cgtttcgagg tcgccagata ttcgccaatt tccccacctg
    11581 cgccgttgac cacgttcacc acccctggcg gcagtaaatc accgacgatt tccattagca
    11641 gcagtacaga aagcggagta agacgtgcgg gtttcagcac cacacagttg ccagccgcca
    11701 gcgcgggagc cattttccag ctcgccatca gcagcgggaa gttccacggg ataatctgcc
    11761 ccaccacgcc taacggttca tggaaatgat aggccacggt ttcgctatcc acttcactga
    11821 tcccaccttc ctgcgcccga atacacgagg cgaaatagcg aaaatgatca atcgccagcg
    11881 gcacgtcggc agcactggtt tcgcgaatgg gtttgccgtt atcccaggtt tctgccgtcg
    11941 ctaaaagttc aaggttttgt tccattcgat cggcaatctt aaacagaatc gccgcgcgat
    12001 cctgcaccga ggtgtgcgcc catttatctt tcactttatg cgcagcatcc agcgccagat
    12061 cgatgtctcg tttgcccgaa gacgccactt cgcacagtag ctgcccggtc accggcgtca
    12121 ggttctggta atactcgcca tcggcaggcg ctacccattc gccaccgata aagttgtcgt
    12181 agcgggcttt taatttgagg gggaaaccat actcgccggg cttaatctgt gctgaagggg
    12241 gattattggt catgatcgtc tccttgcggt gtgaggtata acaagggtag acgtgactgg
    12301 cgaaatcttc gccagtcggt aacagcttta cgacggctat cacgaattta cgggcaaaag
    12361 ctacagcggg tagtgtgcga cgtacactgc ttgctggtta ttaattcttt tctcttgcgc
    12421 gaatccttct ttaacaattg tccgtaaatc ggtccgcgcc gtattatccg agacgctcag
    12481 actcacacta atttcagcaa ctgtgtactc tttccccgga ttggcgagca tcacattcag
    12541 gagcgttacc tggcgcggat tcaggcgttt aagcacacca gagctaaaca gcacactatc
    12601 gagttttgcg cttcgttgtg taatttcatc gatatgctgc tgccagtttt gcagagcacg
    12661 tttaatgaca cccgcctgat attcaaggaa ataagtcaga tccataccat ctgattcagt
    12721 gtactggtag ctggcggcat atttgaccgg tgcagtatga agcaaacggc tgatggaaat
    12781 atatttaaag acatcgtagc ccgatttcag catataccag taaaacagcg cccgactggt
    12841 gcgcccgttg ccatcccgaa acggatgttc atgggccagc ataaaatgca gaatgcaggc
    12901 acggaccaac ggatgaatat acccttcatg gctgtttaac cactggcaaa ctttttccag
    12961 ccgttccggt aacaatgccg cagcgggcgg ttgatgcact atattgccat catagtcggc
    13021 gatcacaaca tcatctgttt cacgaaattc tcccggacga tatttcgcgt cattgattcc
    13081 acccatacca atggcatgta actggcgaat aagcgcaggt gtcagcggtt ctgcgagtaa
    13141 gtgcggaatt tccgccatca gccgggcatt tcccgcaatc atatgctcgt cttcagtgcg
    13201 tggcgaccgc cctgtttcca gcatttttct cgccaccagc gttgtggtat tcgcaccttc
    13261 cagctgggag ctggtgatcg gctcttccag gcgtaactgg cttaactcgg ctccggcccc
    13321 gcgtaaccgt tcagtctgat ctttcagcgc cagcgcggta gcacgtttgt cgacaagctc
    13381 gcacgcttct gcaatcaccg gggtaatgtt gaatccggcc tgttcacccg cttcattgca
    13441 ataattaatt cgttggatcg ccgcgttgcg cgccagacgg gtaagcgtcc acgccacatc
    13501 gacgttttct ccttttttga tgcggtactg caactcatca aaaggcagat aacgtccttt
    13561 ttcatcggta acccggttaa agcgcaggtg ggccaggagt tcctcacccg ataagtgtga
    13621 taaaggaata agtccgggag gtgatttgat agccatgtgc ttatctcaaa aaagcgcaat
    13681 attgagatag tgtatagcag gaaagagaaa ctatctcaaa aaaagcaagt tttgagatag
    13741 ttgaaaaaaa cggctggcaa cacgccagcc gcaggaagaa gggaattaca tcgctgcgcg
    13801 ataaatcgcc acaatttctt cgtgagttgc ctggatcggg ttagtaaagc cgcaggcatc
    13861 tttcagggca ttagtcgcca gaaccgcgaa atcttcttct ttcacgttca ggtcgcgtag
    13921 gcctgccggg atatccactt tcttcgccag ttcacggatg gcgttaatgc aggcttcagc
    13981 accttccacg tcgtttttac ctgtcacgtt cacgcccatt gcagcggcac aatcacgcag
    14041 acgtgcagcg gcgactttgc tgttgaatac ctgaacatgc ggcagcaaaa cggcgttaca
    14101 tacaccgtgt ggcaggttgt agaaaccgcc cagctggtgc gccatcgcat gaacataacc
    14161 cagagaagca ttattgaacg ccataccggc gaggaactgg gcataagcca ttgcttcacg
    14221 cgctttcgca ttactgccat cttcaacggc taacggcagg ttttcggcaa tcatggtcac
    14281 ggctttcagt gcacaagcgt cagtgatcgg cgtggcggca atagaaacat acgcttcgat
    14341 agcgtgcgtt aaggcatcca tacccgttgc ggcggtcagt gacttcggca taccaatcat
    14401 cagagaggag tcattgacag aaagcagcgg agttacatgt ttatccacaa tcgccatttt
    14461 gatgtgacgc gcttcgtcag tgatgatgca gaaacgggtc atttcagagg ccgtacccgc
    14521 cgtggtgttg atggcgatca tcggcagctg cggttttgca gagcggtcaa cgccttcata
    14581 atcgcggatg tcaccgccgt tggctgcaac cagcgcaata ccttttgcgc agtcgtgtgg
    14641 agaaccaccg cctaaagaaa tcacgctatc gcaattattt tctttaagta atttcaaacc
    14701 tgcagcgacg ttttccgtgg tggggttagg ttgggtgcca tcataaataa cgctaaaaat
    14761 attgcgttct tccagtgctt tttgcacatc acccgccata cccaatttcg ttaacatatt
    14821 gtcggtgaca attaaggtac gggtaaatcc ataatctgcc atcatattca ttgcatcatt
    14881 caatgaatca gcaccaataa cattcacgga aggaataaaa aaggttgaag ctgccataac
    14941 acactcctga aagtgtaaaa aagggcgggc agcttactga ggattttcat ttgaaaatat
    15001 gatcaacatt accgtttatt aaaatatttt taataaaaag cacgttacat tcatcaaata
    15061 acgtgccttc ttagcattaa taagaaattt tgtattaatt cat
//
LOCUS       DAZFJL010000116        15101 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFJL010000116 DAZFJL010000000
VERSION     DAZFJL010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.116"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(199..726)
                     /locus_tag="ACHJ8C_003530"
     CDS             complement(199..726)
                     /locus_tag="ACHJ8C_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO5553321.1"
                     /translation="MQHLKNITAGNPKTVAQYQLTKNFDVIWLWSEEGKNWYEEVSNF
                     QADTIKIVYDENNIIVGITRDASTLNPEGFSVVEVPDITANRRADDSGKWMFKDGAVI
                     KRIYTADEQQQLAESQKATLLSEAESVIVPLERAVRLNMATDEERSRLDAWERYSVLV
                     SRVDPANPEWPEMPQ"
     gene            complement(730..2760)
                     /locus_tag="ACHJ8C_003531"
     CDS             complement(730..2760)
                     /locus_tag="ACHJ8C_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5553322.1"
                     /translation="MSTKFKTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPV
                     PDAGQTGLIHEVWRHALNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLI
                     AVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVELTIDTTTVMATQDYVDDKIAEH
                     EQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARK
                     GLVQLSSATNSDSENLAATPKAVKSAYDNAEKRLQKDQNGADIPGKDTFTKNIGACRA
                     YSGALSTEAGNWTTAQFIEWLDSRGAFNHPYWMCKGSWSYANNKIITDTGCGDIHLAG
                     CVVEVMGTKSAITIRVTTPTTSSGGGTTSAQFTYINHGDGYSPGWRRDWNRQGDSMTG
                     TINQDGGSQNAYMSTALCSGTRGGKKYLRKFRGGEGDTIWHETVQGGVVRWATGNTDA
                     QEELSLSSAYGLRSRGEITSLSANGLRIAYGNYGFFIRNDGSSTYFMLTKSGDRLGTY
                     NNLRPLIINDATGAVSMGHGLSVTGDIVSSTKVRAGSGKKITVSSSNTSTKEAAFNLW
                     GNSSRPVVAELGDDAGWHFYSQRNTDNSITFAVNGQVSPSNYSNFDSRYVRDIRLGTR
                     VVQTMQKGVMYEKAGHVITGLGIVGEVDGDDPAVFRPIQKYINGTWYNVAQV"
     gene            complement(2771..3301)
                     /locus_tag="ACHJ8C_003532"
     CDS             complement(2771..3301)
                     /locus_tag="ACHJ8C_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO5553323.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVSIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWETSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNISRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            complement(3294..4202)
                     /locus_tag="ACHJ8C_003533"
     CDS             complement(3294..4202)
                     /locus_tag="ACHJ8C_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFO5553324.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELLWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLT
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELAAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            complement(4207..4554)
                     /locus_tag="ACHJ8C_003534"
     CDS             complement(4207..4554)
                     /locus_tag="ACHJ8C_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO5553325.1"
                     /translation="MTARYLGMNRSDGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTERSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(4551..5186)
                     /locus_tag="ACHJ8C_003535"
     CDS             complement(4551..5186)
                     /locus_tag="ACHJ8C_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO5553326.1"
                     /translation="MNTLANIQELARALRNMIRTGLVVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVTVKASTRVTLDTPEVVCT
                     NKLTTGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGITGSPL"
     gene            complement(5253..5705)
                     /locus_tag="ACHJ8C_003536"
     CDS             complement(5253..5705)
                     /locus_tag="ACHJ8C_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO5553327.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQAAMEFYGGKSPK
                     IASVHQFGLSEENRKDGKKIDYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(5698..6147)
                     /locus_tag="ACHJ8C_003537"
     CDS             complement(5698..6147)
                     /locus_tag="ACHJ8C_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5553328.1"
                     /translation="MRRALTDAVTVLKTSPEMLRIFVDNGSIASTLATSLSFEKRYTL
                     NVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSFDISISL
                     MLTERTLVSEVDGALHVKNIPEPPPPEPVTRPVELYINGELVSKWDE"
     gene            complement(6128..6373)
                     /gene="lysC"
                     /locus_tag="ACHJ8C_003538"
     CDS             complement(6128..6373)
                     /gene="lysC"
                     /locus_tag="ACHJ8C_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFO5553329.1"
                     /translation="MLCAGCTSAPPALTPVIVANACPKVSLCPMPGSDPETNGDLSAD
                     IRQLENALARCASQVKMIKHCQDENDAQTRQPAQGTD"
     gene            complement(6273..6698)
                     /gene="lysB"
                     /locus_tag="ACHJ8C_003539"
     CDS             complement(6273..6698)
                     /gene="lysB"
                     /locus_tag="ACHJ8C_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFO5553330.1"
                     /translation="MSKLMTVLVVLLSLAVAGLFLVKHKNASLRASLDRANNVASEQQ
                     ATISMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRPACTDASDCRQRLPESESLPDAGQ"
     gene            complement(6686..7111)
                     /locus_tag="ACHJ8C_003540"
     CDS             complement(6686..7111)
                     /locus_tag="ACHJ8C_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFO5553331.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVATWAILATVIC
                     VVAGGVGVYATEYVLERYGRELPPESLAVKIVTSLFLQPVPWCRRVAALVVMVVTFIS
                     LVAAGWIFTALIYLVASLFFRLIRTACRQRFEGREPCQS"
     gene            complement(7126..7623)
                     /locus_tag="ACHJ8C_003541"
     CDS             complement(7126..7623)
                     /locus_tag="ACHJ8C_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFO5553332.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(7623..7904)
                     /locus_tag="ACHJ8C_003542"
     CDS             complement(7623..7904)
                     /locus_tag="ACHJ8C_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO5553333.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            complement(7908..8111)
                     /locus_tag="ACHJ8C_003543"
     CDS             complement(7908..8111)
                     /locus_tag="ACHJ8C_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO5553334.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVETVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(8111..8620)
                     /locus_tag="ACHJ8C_003544"
     CDS             complement(8111..8620)
                     /locus_tag="ACHJ8C_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO5553335.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(8720..9463)
                     /locus_tag="ACHJ8C_003545"
     CDS             complement(8720..9463)
                     /locus_tag="ACHJ8C_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFO5553336.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIGGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDTRLLTETLELTATAD
                     MPDEVRAKLHKITGLLLRDGGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     SQPKAATRAPRKTRSVTPAKRGRPKKKAS"
     gene            complement(9467..10540)
                     /locus_tag="ACHJ8C_003546"
     CDS             complement(9467..10540)
                     /locus_tag="ACHJ8C_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO5553337.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRVRNAIIKRQSLDLIMAGFNGVRRAETSDRNS
                     NPMLQDVAVGWLQKYRNEAPARVMSKVTDEEGHTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNKEQDNSEMLAADVIISQKRIGNLPVV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIEENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTLAKATAEPGA"
     gene            complement(10599..11453)
                     /locus_tag="ACHJ8C_003547"
     CDS             complement(10599..11453)
                     /locus_tag="ACHJ8C_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO5553338.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEVETAFSALKQDVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            11627..13399
                     /locus_tag="ACHJ8C_003548"
     CDS             11627..13399
                     /locus_tag="ACHJ8C_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFO5553339.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNVANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAVERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            13399..14433
                     /locus_tag="ACHJ8C_003549"
     CDS             13399..14433
                     /locus_tag="ACHJ8C_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5553340.1"
                     /translation="MSKKKGKTPRPAAKKMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTPFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
BASE COUNT         3363 a         4319 c         3933 g         3486 t
ORIGIN      
        1 attccttcag tgttatctgg ttagcctaaa ccaagtcatt aaagtccgca ggatatcata
       61 tacataaaat gtattgatta gaatttgcac taataaaata atgctttact aaaatctact
      121 ccagacacag agcggcctgt cagattaggc tttactctgt gccatagata tgtaagctca
      181 caccagagct catacaactt attgcggcat ttccggccat tcaggatttg caggatccac
      241 acgactgacc agaacgctgt aacgctccca tgcatccagt cggctacgct cctcatctgt
      301 tgccatattc agcctgacag cgcgctccag cggcacaatc acggattcgg cttcggaaag
      361 caaagttgcc ttttgtgatt ctgccagttg ctgctgttcg tctgccgtat aaatccgctt
      421 aatcacggca ccatccttaa acatccattt acctgagtca tcagcacgtc ggttggcggt
      481 aatatcagga acctcgacaa cgctaaaacc ttcagggtta agcgttgaag catctctggt
      541 gatgccgaca attatattat tctcgtcgta aacaatcttt atcgtgtctg cctgaaaatt
      601 acttacttcc tcataccagt tttttccctc ttcggaccat aaccagataa catcaaaatt
      661 ttttgtcagt tgatattggg caacagtttt tggattaccc gcagtaatat tttttaaatg
      721 ctgcataaat tacacctgtg cgacgttata ccatgtgcca ttgatgtatt tttgtattgg
      781 tctgaatact gcggggtcat caccatcgac ttcaccgaca ataccaagcc cggtaattac
      841 gtgccctgct ttctcataca tcaccccttt ctgcatggtc tggacaactc gagtcccaag
      901 ccggatatcg cgtacataac gggaatcaaa attactatag ttagatggtg atacctgccc
      961 gttaacagca aaagtgatgc tgttatctgt atttctctga ctgtaaaaat gccagcctgc
     1021 atcatcacct aattcagcca ccaccggacg acttgagttt ccccacaaat tgaatgcggc
     1081 ttccttcgtg gatgtattac tgctgctgac cgtgattttt ttcccgctac cggcacgtac
     1141 tttggtactt gagacaatat caccagtaac actcaggcca tgccccattg atacagcacc
     1201 cgtggcatca tttataatca gtggtcttaa attattataa gtacctaatc tgtcacctga
     1261 tttagtcaac ataaaataag tgctgctgcc atcattcctg ataaagaatc cataattgcc
     1321 ataagcaatg cgcagaccat tagcactgag tgatgtaatc tcacctcttg aacggagacc
     1381 ataagcggag ctgagtgata attcttcctg agcatcagta ttaccagtcg cccagcgaac
     1441 taccccgccc tgtactgttt catgccagat agtgtctcct tctccaccac gaaactttct
     1501 gagatatttt ttgccgcctc tggtgcctga acataaggcc gtagacatat aggcattctg
     1561 gcttccgcca tcctgattaa tcgttccggt cattgagtcg ccctgacgat tccagtcacg
     1621 acgccagccg ggggagtagc cgtccccatg attaatgtaa gtgaattgcg cgctggttgt
     1681 accgccacca cttgatgttg tcggcgtggt cactcggata gtgattgcag atttagttcc
     1741 catgacctcg acgacacaac cagccaggtg gatatcacca catccggtat ccgtaatgat
     1801 tttgttattt gcatatgacc aggagccttt gcacatccag tacggatgat taaatgcacc
     1861 acgggaatcc agccattcaa taaactgagc ggttgtccag tttccggctt cagtgctcaa
     1921 agcgccgcta taagcacgac aggcaccgat atttttcgtg aaggtatcct ttcccggaat
     1981 atccgcaccg ttctgatctt tctgaagacg tttttcagca ttgtcatagg cagacttcac
     2041 tgcttttggc gttgccgcga ggttttcaga atcactgttg gtggcgctac tgagctggac
     2101 aagacctttt cgcgctgtgg tggcgtcctg tgcggtatat ttcccgttag ccaggtcata
     2161 tgcggcctta accgctttcg gcgttgcagc cagtgtttca gacgtactgt tggtcgcact
     2221 gcttaactga gtaaaacctt ttgcggtcag cgaggcgtca gggtgacgtc gtgactgttc
     2281 gtgctctgca attttgtcat caacgtaatc ctgcgtcgcc atcaccgttg tggtgtcaat
     2341 ggtcagctcc actgaggcca cactgctgac gatgatgacc atgcgacagg tctgcgaacg
     2401 tcctgagcct tcggcaaggg ctggcttata gctttcggcc atgttcgcca cggcaattaa
     2461 cgttcccgca tcatcgtaca ggccaagctc acgcatccag aaaccgccca cctccggcgg
     2521 aataaccagc tctgcgataa tataattact gtttcgtttg tcctggctga ttttgttcag
     2581 cgcatgtcgc cagacttcat ggataagccc ggtctgtccg gcatccggga caggcaattt
     2641 accaccgcca tccccgacgg ccatcgtggt aatgttgacc ttccgccctc ccggtgcggt
     2701 tgccgctgcc agctttgctg caccggcagt ggtgataacg gttttgaatt ttgtgctcat
     2761 tattcctcac ttatccgggg taaaccgtaa ttacatcgcc gtcgtaagcc acaccaccgg
     2821 cgaacaggta gccgggaatg tcgcgggaaa tgttcaggcc aataaggtga cggcttgcag
     2881 gtttggcatc agcaatcagc cgttccattt cctgatacat tgcctctgtg ataccgcttt
     2941 ccagtacacc gatatcaagc cggaaggtgc cgggcgggtc actggtttcc caccactccg
     3001 tcacgttgat gagatagccc agcggctcca ccacacgccg gattgcacct atagtgcctt
     3061 tatgacagtg gatgaaatag gcatcgcgga taacggcgcg tttggtcgct tccggccact
     3121 tttcatccca tctgtcgacc gaaaacgccc acgccagcca cggcagcaga tttgccgggc
     3181 aggtgtccgg gttccacagc tcacgaatac tgaccggtgt tttttcaatt tccgcacagg
     3241 cttttgcggc ggcgacctca agcggtgatg agccggtcgg cagcagtcgc gaatcactca
     3301 tccgagcctc cggtcacgac gcggtattcg gtacagaaag acgcctgcgt actgttgagc
     3361 acgatgtcgg ccagtggtgc ggccagctcg acacgctgca cgccttccac atgcaaagcg
     3421 gcataaatgg cagacagacg gatgtcgcgc cccagccggt gctgtgccgt gatgtacgct
     3481 tccagttttt tcacggcggc agcgcgtatg ggttcgcttt cgggaccagg gtaaaggtaa
     3541 agcgtggcgt ttatctggta ttcaacgatg gcggcagact gcacggtcac gcggtcggcc
     3601 accggcctga catcctcgcc attaagggcg ttacgcacca ccgccagcag gtcttcggat
     3661 gcgacaccgt tattttcacg tgacagcaca gagatggtga cgcaggccgg agacggactg
     3721 gtgacagaga tatccgcgac acgcccgtca gcactgcgac catgatactg ataggcaccc
     3781 accgacccgg cgacgcttaa gccttcaaag gcctgctgaa tacgcagacg ataatcggtg
     3841 tcagattcca tcacggccgg tgtcggcggg atggtcgaat catctgccgg ggtgatagtc
     3901 agacgcgtgg tgttgtaatt ggcaccaatc acatcaaggt cattaccggc tgcacaggcc
     3961 agcatcaccg cccgtgcagc ctcattcaca cgctgacgcc agagaagctc acgataagca
     4021 ttttcctcca gcagtttgac gagaggctcg gattccagcg tcagggtacg ggcgaccgcc
     4081 tcctgctggt cttccgggta aagggaaatc agtgtcgcct tgcgttcggc aagaatggtt
     4141 tcaaagtcca gctcctcaac cacatccggt gcgggtagct ggttcaggtc gataatcggc
     4201 atggtttcaa ctcacaggga tggttaacga aagtggctgg ccggtgtcgt tgtgctgacc
     4261 ggttaacgtg accgtcattc gcccgtcaaa actgcgctca gtggtgacgg atgacagggt
     4321 gacgcggggt tcccatttca gcaccgccat gtaacaggca accttaatct gcaactcaag
     4381 cgccggggtc tgcggctggt caatcattga tgccagtaac gagccgtaat cacgacgcat
     4441 cacccgtgag ccgaccggtg tgcgcaggat atcgccgata ctctggctga tatgctcaag
     4501 gtcagtgaca gtcaggccat cactgcgatt cattccgaga taacgtgctg tcataaagga
     4561 ctcccggtta tgccgccgct gtcgccgggg tgtttatggg tatgcagtac cttaccgttt
     4621 gatgagagtt caccgccggt gtgttcaatg ttgccgcgca tcgtcccgcc cttctgcact
     4681 tccagcgtgc cggtagtcag tttgttagtg cagaccactt ccggtgtgtc cagggtgaca
     4741 cgggttgacg ctttcaccgt gaccaccggc accgtggcag taacagaatc agaagccgtc
     4801 acgctggccg ttttaattcc gcttaccgta agtgcactgg tttcgggttc atactcaatc
     4861 accgccccgt cagggaaacg gatatgcagg gcatccgccg acgcagacgg cgcggggtta
     4921 tcgccggaat aaatccccgg cagaacgaac gccgtgtcaa gttcaccacc cacggccaga
     4981 atcagcacct gttcccccac ggaaggtgcc caccatgtgc gcgaacgccc ggcgcgatgg
     5041 gtcagccact gaagccagtc ggtgcacatg ccgccggtct gcacacggca gcgaccggcg
     5101 ttaaggtcag tttcgacgac aaggccggtg cgaatcatgt tgcgcagtgc gcgcgcgagt
     5161 tcctgaatat ttgcgagagt gttcataacg ggaaggatgc cgccgggtca taccggcggc
     5221 aatgtgacga tgaggtgtcg ggaatggcac aactaacggt cgaggtgagc caggataatc
     5281 tcttcaatca tctgcacatc ctcaccggta aagccgagca gaggacgcgc cggataatca
     5341 attttcttac cgtctttccg gttttcttcc gacagaccga actgatgcac actggcgatt
     5401 ttcggtgact tcccgccgta aaactccatt gctgcctgtt cagggctggc gcggatatgc
     5461 aaaaaacgac tggtgataag tttcgcaaac atttttcgct taacgcgacc ggtctttttt
     5521 ctggcgctct gctgctggcg tggtgcgtag ggtgtgccgt ccggggcttt ctgtgccatc
     5581 acccggcgct gctgactctg ccgcagacgt ttcgccagtt cggcgctcag tcgccgacgc
     5641 cctgacggtg acagcgattc aataagtccg gtcagccggt cttcaaaacg cttaaactca
     5701 ttcatcccac ttgctcacca gttcgccatt gatataaagc tccaccgggc gggtgaccgg
     5761 ctccggcggc ggaggttccg ggatattctt cacatgcagt gcgccgtcca cctcactgac
     5821 cagcgtgcgc tcggtcagca tcaggctgat gctgatatca aagctgctgt cattgttgat
     5881 gtccgcataa aacgtgaagc cctttttctg gccttcgtcg gtggtcatga tgtcgggctg
     5941 attttcccgc agccacgcca gcaccggcac gatgagcagg tcaaaatcac cggtaaagtc
     6001 ggtcacaatg acattgagcg tgtaacgctt ttcgaatgac agcgacgtcg ccagtgtgga
     6061 ggcaatactc ccgttatcca cgaatatccg cagcatctcg gggctggttt tcagcaccgt
     6121 gacggcatca gtcagtgccc tgcgcaggct gtcgggtttg agcgtcgttt tcgtcctgac
     6181 agtgtttaat catttttacc tggctggcac agcgtgccag cgcgttctca agctgccgga
     6241 tatcggcact taaatcgccg ttcgtctccg ggtcactgcc cggcatcggg caaagactca
     6301 ctttcgggca ggcgttggcg acaatcactg gcgtcagtgc aggcggggcg ctggtgcaac
     6361 cggcgcacag catcaggcag gtcagcaccg taccagcggc gaaaatcttc gttttcattg
     6421 agtaatctcg tgatggtttt ctcgcgctgt gcttcacgct tcgcggcgtt ctccagttcc
     6481 tgacgcagag ccacctgcgc cagctcgttt ttgtctgccc tggtgagcgc aacatgaagc
     6541 tgatttttca gcatggagat agtcgcctgc tgttcactgg cgacgttgtt cgccctgtcc
     6601 agcgaggcgc gcaggctggc atttttatgt ttcaccagaa acagaccggc caccgccagt
     6661 gataacaaca caaccagcac agtcatcagc tttgacatgg ttcccgcccc tcaaaacgct
     6721 gacggcaggc cgtacgtatc agccggaaaa acagcgacgc cacaagataa atcagcgcag
     6781 taaaaatcca cccggcagcg accagcgaga taaacgtcac caccatcacc accagagccg
     6841 ccacccgtct gcaccacggc accggctgca aaaacagcga cgtgacaatc ttcacggcca
     6901 gcgattccgg cggcagctcc cgtccgtagc gttccagtac atactcagtg gcatacacac
     6961 cgacaccacc ggcaaccaca cagataaccg tcgccagaat cgcccaggtg gcaacaaaac
     7021 tgacggccac gctctgcggg taaatcaggg acagtgccag catcagcgcc agcgacacgt
     7081 tcagcatcag tgaaagggat aatttcttca tggtgtttac tccgtttaag ccggtacgcc
     7141 gccagcggta cgccagacgg tgaccagttt ttccagtgaa tgctcacgct gaccgtaacc
     7201 ggctcccggc agggacgccc agatattgcg acagcgtgaa atggcgcgct caatgcgtcc
     7261 cgcccggatg tcatccagtg caccgcgttc gcggatcaac tgaatggcga gtctgtcctg
     7321 tgacaacgga ctgaaatccg gcagggcaag ctgtttgcgg taatgcggcc agaacaggta
     7381 aagctgctga tagcgaccgg aggccgtgga tttttcaccg cgacggttaa acaccttcgc
     7441 cggtcggcca tgtgcgaacg ggtggtcact gtagtcggtg aaaatttccg gcttcccgtc
     7501 cagtccggtg actatcacgt catagccccg gtttttcgtc agcggatgat tcgccgtccc
     7561 ttcggatacc gccagcatgt cgagaaaggc ggcgatattc tgatgcgtgt taattaccgg
     7621 cattacggtt tccccctgcc cttaaagcgg cgctgaatgg caatctcaat cacctgataa
     7681 ccggcgatac ccagcatgga gccgatgccg cacaccgcag gcagtgacag gtcaggaaac
     7741 tgcaccagaa caacaccggc aaccatcgag acaaaaccac cgagcaacat gcgcccgata
     7801 aacagacgcg gggtgatggg ttcaccaccg gcaagcacct tgccgacaac aatcagcacc
     7861 ccaatcatga aaagcgacag gacgcttttt tcttctgctg tcatgcgtta ctcccacaga
     7921 ttgacagttt cagccacggg cgcggtctga acgtcgggca gttcgacggc ggtgccgtgt
     7981 ggcagcaccg cccccagttc agccagtccc ggatttgcgg cgagcacagt ctcaaccacg
     8041 ccctcagtgc gcccgtaata ccggacacaa atggcgtcga gcgtgtcgcc ctgtagcgca
     8101 aaggtcttca tcagatttga ctcacgatgc agcgcggctt gtcctggatg cgcgccactg
     8161 cccagcgcat atcccgccac agctcatcaa tggtgctgtc aatgctgtcg gccttcttgt
     8221 cgcctttcgc actggcatcc acaccgcgat aacgttcata aagtgacgcg gtcgccatcg
     8281 cacacacggc gcgctcgtag taaaaaactt tgatgctttc accgtcgata tcgtctgccg
     8341 ggacgtcagc cagacgcgta aaaccggcgg caattttctg ttcgcggtac tcgtacagct
     8401 ccgcattcgt ctccgccatg cctgacttga tggcctcacg cagacgggcg ggggcgacgg
     8461 tctgctcaag gcgcatacgt tcccggacgc gcttcgggtc aatatcggga aaaaagaacg
     8521 tgtttttaat caccggctcg tcgcctgccg gttgcgggat gaccaccgta ccctcaccgg
     8581 acacgggagc ctcctttcgc ggaataatca gcgtcatcat gactacctca gaaaagtcgg
     8641 gcggtggacg ccggtacagt gtcaggtgat tcacccacac tgaccggcgt gccgccctgg
     8701 cgcggggcgc attcggttgt taactggctt tctttttcgg gcgtccacgt tttgccggtg
     8761 tcacgctccg ggtcttacgc ggggcgcggg tggccgcttt gggctgcgac tccggcttcg
     8821 gtttcagctc ccgctccagt cgttcaatct cttttttgac acctgcctga cagtcgagct
     8881 gtgtcgcacg ttgcaggtga gccagcgcac cggcggcatc accaccatca cgcagaagca
     8941 gaccggtgat tttgtgcagc tttgcgcgca cttcatcagg catgtcagcc gtggcggtca
     9001 gttcaagggt ctccgtcagc aggcgggtat ccacagactc accggcagcg tgggcgcgca
     9061 tggccgctag cgccacctcc tcggtgaaca tgtatggcgg ggtgcggcgg tgtttacccg
     9121 gcatggtcag accgtacttc agggcataac gggcaatctc cagcgcaccg ccaatatcgc
     9181 cggtatccag acgccacagc atgaccgtca tcagaatgtc atcctgtgca cctttgccct
     9241 gctccagcac gccgttcacc cacggcaacc agaacggcag cagttcgcgt tttttcgcgg
     9301 ccttcagctc ttttgaataa atcgctttca gtgtgcgctg gtctgcggcg agcttaacca
     9361 gcatctgctc atagacagtt gcatgtcgca gcggggcggc ttcccgctgc gcggtcatcg
     9421 ctgccgagac ccgcatcatg tggcgctgtg cgggactcgt catcggttac gctcccggct
     9481 ctgcggtcgc tttagccagt gtggagaaat caccgacctt aattttttcc accagacaac
     9541 cggcggcgta gtcctccacc acgtaatcaa tgttcattga ctcgtagttc tccacgcggt
     9601 cgagtttcgg gttttcctca atcacgcggc gatggctgtc atccatgtag tagatggaca
     9661 ggttttccag cttcgtgatg agcatcgcat ccgccgggaa gtacgggacg cgtaccactg
     9721 gcaggttgcc gatgcgtttc tggctgatga tgacgtcagc ggccagcatt tcgctgttgt
     9781 cctgctcctt gttgacgatg ggaaaatact tgtccgccag tagctgacgt cccacaatca
     9841 ccacaaggtc agggtcttcc tgataccacg gctcaatcag gttgttggtc gcatccatca
     9901 ccagtgcatc aaggctggca taatcaccgc ccttacccac gcggatgacc tcagaggtgg
     9961 tgtgcccttc ctcgtcagtg accttgctca tcacgcgcgc cggggcttca ttgcggtatt
    10021 tctgcagcca gccgaccgcc acatcctgca gcatcggatt actgttgcgg tcagaggttt
    10081 cggcacgcct cacgccgtta aaaccggcca tgattaaatc aagggactgg cgtttgataa
    10141 tggcgttacg gacacggagc tggaaatcct gataacgcgc ccacaggtcc agcgttttgt
    10201 agcggatata aaaatcgaag ttaatctggt cgcattcgta cttgtttgac gccagcttcg
    10261 agaagtcctt cggctgacgc tcggtgccac cggcggtgtc ggtggtgctg gcgatggagc
    10321 cggtgacacc gatgccaatt ttttcccctt tcatttcgct gaccggcaca atgttgatgc
    10381 gggtcagaaa gtcagaggac tcctgcatgg tgttcatcag ggtctgggtg accgacggtt
    10441 caacggtgaa ttttttcgac acatcaccgg cgtcgatgcc gttcagttcg gcaacacggg
    10501 acaggtaggc attaaattta aagcgggttt cctggcgcat agtttttcct gaaattaagg
    10561 gttaatcgtg aaggttttcc cggactgact gacgccggtc agcagttcgt catcagggcg
    10621 tcaccgccac caccggtggc cttgctgcgg cgctgctggg tcagactttc ggtgtggtcg
    10681 agactgtttt tcaggcgggt gaatgcctgg ctggtttcat ccgccctgtc agtcacatcc
    10741 tgcttaagtg cggaaaaggc ggtttccacc tcagcgaggc gctgctcagt ggcgctcagt
    10801 ttttcctgca catgttcagc aacagcggtc accgcttcat gcacgtcatt cagacgggcg
    10861 tcatcgctgg cctgtttgcg gccaaaaatg gatttcacct tttcggtcag ggcggtgaac
    10921 acggtttcag gcaggtcttc aaattccagc tcaacaggcg ttgccactga aatcaggttt
    10981 tcagggctta atttgaagcg gttcagaggg ttgtgttttg ccgtgcggca gaattccagg
    11041 tattccgtgc cgaggcttgc cgggtcatcg gtgacggcca gacccaccag ataacatttg
    11101 cctgtattgg caaagttcgg ctgaatttcc attgaggtat agaccttctg cgcggccttg
    11161 ttcatcgcga taaggtcatc ggtcggggtg attttcgcaa acagcgccca tttgcctttc
    11221 agcgccgaat catcgtcaat tttttcggcc ttcagttcgg ccacatcgcc ataacgttta
    11281 aaaatgccgt caggcaggat gccgcgcaga tgttccaggt taatgcggca accatagaca
    11341 cgcgggtcaa aggtttcggc catttcctga atatcctgcg cactgatgac acgcccgtca
    11401 caggtgtcac cctcaacgcc gatacgaaag aattttgaga ctttttttgc cattgtcagg
    11461 agtcctgaat agtgattaga ggagtcacat gtcggcatca gtttcccgac gatgcgcatc
    11521 ctccgccatc agtcccggat ggcttatcac tgacacaaca gcaccttagc gaatcgcggg
    11581 gcgcgactca gtagccttgc cgtgtattca tcacggcgag gtattcatga ccatcaccac
    11641 agacaccact cttttacacg acccgcgtcg tcaggcggcg ctgctgtact ggcagggatt
    11701 ttccgtgccg cagattgccg ccatgttgca gatgaaacgc ccgacggtgc agagctggaa
    11761 acagcgcgac ggctgggaca gcgttgcccc catcagccgt gtcgaaatga gtctggaagc
    11821 gcgactgacc cagctcatca tcaaaccgca gaaaaccggc ggtgacttca aggaaattga
    11881 cctgctcgga cgccagattg aacgactggc gcgggtcaac cgctacagtc agaccggcaa
    11941 cgaggcagac cttaatccga acgtcgctaa ccgcaacaaa ggcgggcggc gcaaaccgaa
    12001 aaagaatttt ttcagtgacg aagccatcga aaagctggag cagattttct ttgagcagtc
    12061 tttcgactat cagttgcact ggtatcgcgc cgggcttgag caccgcatcc gcgatatcct
    12121 gaaatcccgc cagattggcg cgacgtttta tttttcccgc gaggcgctgc tgcgcgccct
    12181 gaaaaccggc cataaccaga tttttctgtc ggccagtaaa acgcaggcgt atgtgttccg
    12241 cgaatacatc atcgcctttg cccgtctggt tgacgttgac ctgaccggtg acccgattgt
    12301 cctgggcaat aacggcgcaa aactaatttt tctcggcacc aactccaaca ccgcacagag
    12361 ccataacggc gacctgtacg tcgacgagat tttctggatc ccgaattttc aggtactgcg
    12421 taaggtggca tcaggtatgg cctcacagag tcacctgcgc tcgacctatt tctccacccc
    12481 gtccacgctg gcgcacgacg cctacccgtt ctggtcgggt gaactgttca accggggacg
    12541 cgccagcgcc gtcgaacgcg tggaaatcga cgtcagtcat aacgcccttg ccggtgggct
    12601 tctctgtgcg gacggccagt ggcggcagat tgtcaccatt gaggacgcgc tgaaaggcgg
    12661 ctgcacgctg ttcgacattg agcagctcaa acgtgaaaac agcgccgacg attttaaaaa
    12721 cctgttcatg tgtgaatttg ttgacgacaa ggcgtcggtg ttcccgttcg aggagctgca
    12781 acgctgcatg gtcgacacgc tggaagaatg ggaagactat gcgccgtttg ccgcgaatcc
    12841 gttcggctcc cgcccggtat ggattggtta cgacccgtca caccgtggcg acagtgccgg
    12901 atgcgtggtg ctggcaccgc cggtggtggc cggaggtaaa ttcagaatac ttgagcgtca
    12961 ccagtggaaa ggcatggact ttgccaccca ggctgaatcc atccgcaaac tcaccgaaaa
    13021 atacaacgtc gaatacatcg gaattgatgc caccggcctc ggtgtcggcg tgttccagct
    13081 cgtgcgctcg ttctatcccg ccgcgcgcga catccgctac acgccggaaa tgaaaaccgc
    13141 aatggtgctc aaggcaaaag acgtcatccg ccgtggctgt ctggaatatg acgtcagcgc
    13201 caccgacatc accagctcgt ttatggctat ccgcaagacc atgaccagca gcggacgcag
    13261 cgccacctat gaggccagcc gcagcgagga agccagccac gccgacctcg cctgggcgac
    13321 catgcacgcc ctgttaaatg agccactcac cgcgggtatc agcactccgc tgacatccac
    13381 cattctggag ttttactgat gagcaagaaa aaagggaaaa caccgcgacc agcggcaaaa
    13441 aaaatgaccg ccagcgcccc gaaaatggag gcattcacct ttggtgaacc ggtgccggta
    13501 ctcgaccgcc gtgacattct ggattacgtc gagtgcatca gtaacggcag atggtatgag
    13561 ccgccggtca gctttaccgg tctggcaaaa agcctgcgtg ctgccgtaca tcacagctca
    13621 ccgatttacg tcaaacgtaa tattctggct tcaacgttta ttccgcaccc gtggctttcc
    13681 cagcaggatt tcagccgctt tgtgctggat tttctggtgt tcggtaatgc gtttctggaa
    13741 aagcgttaca gcaccaccgg taaggtcatc agactggaaa cctcaccggc aaaatatacc
    13801 cgccgtggtg tggaggagga tgtttactgg tgggtgccgt ccttcaacga gccgacacct
    13861 ttcgcgcccg gctccgtgtt tcatctgctg gagccggata ttaatcagga gctgtacggc
    13921 ctgccggaat atctcagcgc ccttaactct gcctggctga atgagtcggc cacgctgttc
    13981 cgccgcaagt attacgaaaa cggcgcacat gccggataca tcatgtacgt cactgatgcc
    14041 gtgcaggatc gcaacgatat cgaaatgctc cgcgaaaaca tggtcaagtc gaaaggccgc
    14101 aacaacttta aaaatctgtt tctctatgcc ccgcagggga aagccgacgg cattaaaatt
    14161 atcccactca gtgaagtggc aacgaaggac gattttttta atatcaaaaa agccagcgcc
    14221 gctgacctgc tggacgcgca ccgtatcccc tttcagttga tgggcggcaa gccggagaac
    14281 gtcgggtcgc tgggtgatat tgagaaagtg gcaaaggtct ttgtccgcaa tgaacttatc
    14341 ccgttacagg acaggattcg ggaaataaac ggctggctcg gccaggaggt catccgcttt
    14401 aaaaactact cactggacac tgacaacggc tgaacatcgc cgcctgcggg cggctttttt
    14461 acaccccgtc atcacgccct cacacgctca ccaccgcaca aaacaccccg cagacacacc
    14521 aacgccccgg cgcacaatct aaacgccatc aagacgcgct cagacgctga aaaaataaaa
    14581 tcagcaccac cgccagcgcg cagtgctttc cccgcctcgc ccgcccgctt catgtggagg
    14641 ttttaatgcg gttgcatgac atctctgaat ccaaactagc tgtgatgtcg gacgccgaaa
    14701 acgtataatt ctgatacatg caaagctatg cacctaatgc atgcataact ctgatcttat
    14761 gaaatgacat aaagcagcat cacagaaggt gactttaaag ctttgtgttg tcactggaag
    14821 gaaatgaaga tatgagtgat aaattgagca gtgctatgtg atgggacaaa gataatctta
    14881 ccgaaacagc acctcctgtt catcttaagt gtaaatgtca tgagcaccaa acttctcact
    14941 acatccattt tttagcgata aaactccaac atgttataat aaccatgcat tcttactcaa
    15001 aacatgatac cttcaaaaca tttttcaata acactaacaa aaaacaaaat gaaacaccaa
    15061 aaagttaata tagtcaaaaa gaatggtgaa ttctctttaa g
//
LOCUS       DAZFJL010000117        14956 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFJL010000117 DAZFJL010000000
VERSION     DAZFJL010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.117"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(134..1186)
                     /gene="fbaB"
                     /locus_tag="ACHJ8C_003550"
     CDS             complement(134..1186)
                     /gene="fbaB"
                     /locus_tag="ACHJ8C_003550"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO5553341.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            1443..2720
                     /locus_tag="ACHJ8C_003551"
     CDS             1443..2720
                     /locus_tag="ACHJ8C_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO5553342.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWAGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            2717..3721
                     /locus_tag="ACHJ8C_003552"
     CDS             2717..3721
                     /locus_tag="ACHJ8C_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO5553343.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRISPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVNSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNTIDPALKAELDTVNQLDFNRYATALAKYRQQREAI
                     "
     gene            3718..4683
                     /locus_tag="ACHJ8C_003553"
     CDS             3718..4683
                     /locus_tag="ACHJ8C_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO5553344.1"
                     /translation="MSGARLHTLLPELTSRQPVMVIGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEDLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(4657..5403)
                     /locus_tag="ACHJ8C_003554"
     CDS             complement(4657..5403)
                     /locus_tag="ACHJ8C_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5553345.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRNGVLEHG
                     NILPGERNLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(5455..6273)
                     /locus_tag="ACHJ8C_003555"
     CDS             complement(5455..6273)
                     /locus_tag="ACHJ8C_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO5553346.1"
                     /translation="MQLRITSRKKFTVLLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQTFVDGIKETP"
     gene            complement(6338..7138)
                     /gene="thiD"
                     /locus_tag="ACHJ8C_003556"
     CDS             complement(6338..7138)
                     /gene="thiD"
                     /locus_tag="ACHJ8C_003556"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO5553347.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(7135..7923)
                     /gene="thiM"
                     /locus_tag="ACHJ8C_003557"
     CDS             complement(7135..7923)
                     /gene="thiM"
                     /locus_tag="ACHJ8C_003557"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO5553348.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMSAAVEQATRSQTPWTLDPVA
                     VGALDYRRRFCVELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENIASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLAQEVQA"
     regulatory      complement(7958..8054)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(8146..8418)
                     /gene="rcnR"
                     /locus_tag="ACHJ8C_003558"
     CDS             complement(8146..8418)
                     /gene="rcnR"
                     /locus_tag="ACHJ8C_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO5553349.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            8539..9363
                     /gene="rcnA"
                     /locus_tag="ACHJ8C_003559"
     CDS             8539..9363
                     /gene="rcnA"
                     /locus_tag="ACHJ8C_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO5553350.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSLGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            9582..9920
                     /gene="rcnB"
                     /locus_tag="ACHJ8C_003560"
     CDS             9582..9920
                     /gene="rcnB"
                     /locus_tag="ACHJ8C_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO5553351.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(10002..11036)
                     /locus_tag="ACHJ8C_003561"
     CDS             complement(10002..11036)
                     /locus_tag="ACHJ8C_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO5553352.1"
                     /translation="MEIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQIQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLESIPVSQLPATGN
                     KTTINSKKGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGTPVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(11052..13532)
                     /locus_tag="ACHJ8C_003562"
     CDS             complement(11052..13532)
                     /locus_tag="ACHJ8C_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO5553353.1"
                     /translation="MLRMTPLASAIVALLIGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLEDNQPLPGPYDIDIYVNKQWRGKYEIIVKDNPQETCLSREMIKRLGINTDSFASGK
                     QCLTFKQLIQGGSYTWDIGVFRLDFSVPQAWVEDLESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLQGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSIQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASHAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQYQGNETTAGVNL
                     TWNAPVATVNGSYSQSSAYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENYLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESKNYICQ"
     gene            complement(13548..14222)
                     /locus_tag="ACHJ8C_003563"
     CDS             complement(13548..14222)
                     /locus_tag="ACHJ8C_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5553354.1"
                     /translation="MKGLLALLISSMVLPAHAGIVIYGTRIIYPAEHKEVMVQLMNQG
                     NRSSLMQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKVTFKKLLINRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(14303..14845)
                     /locus_tag="ACHJ8C_003564"
     CDS             complement(14303..14845)
                     /locus_tag="ACHJ8C_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5553355.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDGAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTADGQYAIPFKAKYLKLT
                     DNSVNSGDVLSSLIMRVAQD"
BASE COUNT         3697 a         3757 c         3474 g         4028 t
ORIGIN      
        1 aaattaatca gaatgacagg caaaaaaaac ggcctcccgg gggaagccgt aacgaagtgc
       61 gcgtgttttt atgccggatg caacgtaaac gccttttccg tcctacgggg tggtgctgtt
      121 cgtatgaggc gcatcaggcg atagtaattt tgctatcgag ataaacgtcc tgcacggcgt
      181 taatcagttt cacgccgtca gccatcgatt tcttgaacgc cttacgtcca agaatcagcc
      241 ccattccgcc tgcgcgtttg ttgataaccg cagtacgcac tgcatcgctg aggtcagttt
      301 caccgcctgc agcaccgccg gagtttatca acccggcccg gcccatatag cagttagcta
      361 actgataacg caccaggtca atcgggtttt cactggtcag tttgctgtag actcgatcgt
      421 cggtataacc gtaattaatc gctttatagc cgccgttatt ttccgccatt ttttgtttga
      481 cgatatctgc acctatggtt gccgccagat ggttcgcctg accagtcagg tcggcggaaa
      541 catggtaatc aacgccatct ttcttaaagg cggagttacg caaataggcc cacagcactg
      601 tcaccatgcc cagctcgtgc gcacgttcaa aagccgcaga aatttcttca atctggcgac
      661 gtgactcttc cgagccaaaa tagatagtcg caccaaccgc caccgcgccc atgttgaacg
      721 cctgctccac gctggcatac agcgtttgat cgtaggtatt cgggtaactt agcgtctcgt
      781 tgtgattaag tttgacgagg aatggaatgc gatgcgcgta acgccgcgat accgatgcca
      841 gcacgccgta agttgacgcc acacagttac agcccgcttc gatcgccagt tcaacaatgt
      901 ttttcgggtc aaagtagagc gggttagcgg caaatgaagc tccggcagag tgctcaacgc
      961 cctggtcaac cggcagaata gaaagatatc ctgtgccagc cagacgcccg gtgttgtaca
     1021 acgtctgcat attacgtaac accgctggcg ggcgattatt gtcaatcatc acgcggtcta
     1081 cgtagtcatg tccggggaga taaagctggt cagaaggaat agtcatacaa cggtgctgta
     1141 aaaggttgtc ggcgtctttg ccaagcaact gcgcaatatc tgtcattact atgctcccgt
     1201 aaattccgat tggatatcgg ctatggattg tcctggcccg ccttttgcgg gcaatcataa
     1261 tcctggtcgt tacgggcgag tttttccatc ttttggatgt tttttcagcg ttttctgttg
     1321 gctcgattca tcagaaaaaa tgttaccgcg gtcaagtttt caccgcaaag gtatttaaaa
     1381 ggtattacta agtggtattg tcatcgcgta ccttacatta cctgtcatga aggaattaaa
     1441 agatgaaaac aacagcaaag ctgtcgttca tgatgtttgt tgaatggttt atctggggcg
     1501 cgtggtttgt gccattgtgg ttgtggttaa gtaaaagcgg ttttagtgcc ggagaaattg
     1561 gctggtcgta tgcctgtacc gccattgcgg cgatcctgtc gccgattctg gttggctcca
     1621 tcactgaccg ctttttctcg gcgcagaaag tgctggcggt attgatgttc gctggtgcgg
     1681 tgctgatgta tttcgccgcg caacagacca cttttgccgg gttcttcccg ttactgctgg
     1741 cctactcgct aacctatatg ccgaccattg cgctgactaa cagtatcgct tttgccaacg
     1801 tgccggatgt ggagcgtgat ttcccgcgca ttcgtgtgat gggcactatc ggctggattg
     1861 cctctggtct ggcatgtggt ttcttgccgc aaatgctggg gtatgccgat atctcaccga
     1921 cgaacatccc gctgctgatt actgccggaa gttctgctct gcttggtgtg tttgcatttt
     1981 tcctgcccga cacaccgcca aaaagcaccg gcaaaatgga tattaaagtc atgctcggcc
     2041 tggatgcgct gatcctgctg cgcgataaga acttcctcgt ctttttcttc tgttcattcc
     2101 tgtttgcgat gccactggcg ttctattaca tctttgccaa cggttatctg accgaagttg
     2161 gcatgaaaaa cgccactggc tggatgacgc tcggccagtt ctctgaaatc ttctttatgc
     2221 tggcattgcc gtttttcacc aaacgctttg gtatcaaaaa ggtattattg cttggtctgg
     2281 tcaccgccgc gatccgttat ggcttcttta tttacggcag tgcggatgaa tatttcactt
     2341 acgctctctt gttcctcggc attttgctgc acggcgtaag ttacgatttt tactacgtta
     2401 ccgcttacat ctatgtcgat aaaaaagccc ccgtgcatat gcgtactgcc gcgcaggggc
     2461 tgatcacgct ctgctgccag ggcttcggca gtttgctcgg ctatcgtctt ggcggtgtga
     2521 tgatggaaaa gatgttcgct tatcaggaac cggtaaacgg actgactttc aactgggccg
     2581 ggatgtggac tttcggcgcg gtgatgattg ccattatcgc cgtgctgttc atgatttttt
     2641 tccgcgaatc cgacaacgaa attacggcta tcaaggtcga tgatcgcgat attgcgttga
     2701 cacaagggga agttaaatga aaacagaacg tattctcggt gctctttatg ggcaggcgtt
     2761 aggggatgcg atggggatgc cctccgagct ttggccgcgc agtcgcgtca aagcacactt
     2821 tggctggatt gaccgttttc tgcctgggcc aaaggagaat aacgcagcct gttattttaa
     2881 ccgcgccgaa ttcaccgacg atacctcgat ggcgctgtgt ctggcggatg cgttactgga
     2941 acgtgaaggc aagatcgatc cggatctgat tgggcgtaat attctcgact gggcgctgcg
     3001 tttcgacgcc tttaacaaaa acgtactagg tccgacctcg aagattgcgc ttaacgccat
     3061 tcgcgacggt aaacccgttg ctgaactgga aaataacggc gtgaccaacg gcgcagcgat
     3121 gcgcatctcg ccattaggtt gtttgcttcc ggcgcgtgat gttgattcct ttattgatga
     3181 tgtggcgctg gcctccagcc cgacacataa atccgatctg gcggttgcgg gggcggtagt
     3241 catcgcatgg gcgatttctc gtgccattga cggagaaagc tggtcagcaa ttgttaattc
     3301 actgccttca attgcgcgac atgctcaaca aaaacgcatc actaccttca gcgcctcact
     3361 ggctgcacgt ctggagattg cgctgaaaat tgtgcgcaat gctgacggca ccgaatccgc
     3421 cagcgaacag ctttaccagg tcgttggcgc aggtaccagc actattgagt ccgttccgtg
     3481 cgccattgcg ctggttgaac tggcacaaac cgacccgaac cgttgcgccg tcctgtgcgc
     3541 taaccttggc ggcgacacag acaccatcgg tgctatggcg acggcaattt gcggcgcgtt
     3601 gcatggcgtt aatactatcg atcctgcgtt aaaggcggaa ctggatacag taaatcagct
     3661 tgatttcaac cgctatgcca cggcgctggc gaagtatcgc caacaacggg aggcgatatg
     3721 agcggcgctc gattacacac gctgctgcct gaattaacct cgcgtcagcc agttatggtg
     3781 attggcgcgg cggtcattga tgtgatcgcc gacgcttatg ccctcccctg gcgtgggtgc
     3841 gatatcgaac tgaaacagca gagcgttaac gttggcggct gtgcactgaa tattgccgtg
     3901 gcattaaagc gcctcggcat cgaagcgggt aatgccttgc cgctcggtca gggggtgtgg
     3961 gcggagatta ttcgcaaccg gatggcaaaa gaggacttaa tcagcctgat cgataacgcc
     4021 gaaggtgata acggctggtg tctggcgctg gttgagcctg atggcgaacg cacttttatg
     4081 tcattcagcg gtgtggaaaa tcagtggaat cgccagtggc tggcgcgatt aaccgttgcg
     4141 cctggcagcc tgctctattt ttccggttat caactggcct cgccctgcgg cgaattgtta
     4201 gtggaatggc tggaaaagct gcaagacgtg acgccgttta tcgattttgg cccacgtatt
     4261 ggcgatatcc cggatgcatt actggcgcgg atcatggcct gtcgaccttt agtgtcactc
     4321 aatcgtcaag aggctgagat tgccgccgaa cgttttgctt tatccgcaga gataacaaca
     4381 cttggcaagc aatggcagga gaaatttgcc gcgccgttga tcgttcgcct cgataaagaa
     4441 ggcgcatggt atttcagcaa cgacgcttct ggctgcattc cggcatttcc aacgcaagtt
     4501 gtagacacca ttggggcggg cgacagtcat gccggtggcg tacttgccgg gctggcctct
     4561 ggtctgccac tggcggatgc cgtattactg ggcaatgcag tggcgtcgtg ggttgtcggg
     4621 catcggggcg gtgattgtgc gccaacgcgc gaggaactac tcctcgcaca caaaaacgta
     4681 tagatcgctg cgacagtggc tgatgctgta ctcaataggc cgttgctgtt ggtcaagcgc
     4741 cacttgcttg atcaccagca ccggtatttt gctatctaac tgaatatgtg actgaaactc
     4801 ggcatccggc atccgggcgc taacgcggga acgcgtacgt tgtgggtaaa tgtgctggct
     4861 gcggaagtag tcataaagag aaatcccgat ggcatcaaca tcatgaatta aatgtgccgg
     4921 aacccacgat tcctcaatcg ataccgcctc ttcatcgaca tagcgaatac gcttaagcaa
     4981 aaagacatca cttcccgctt cgaccgccag ctgctgcgcg acttcttcgg gacatttcac
     5041 gacacgcttg ttaacccata gcgtatcggg ctttttcccg cgcaatacca cctgctgaga
     5101 aaacccccgc gcttctttca gcgagtattc gaagatgttg ttgatctgcg tgccgtaacc
     5161 acgcgaacgc gtcaccacgc cctcttcttc cagcgcctgc atcgccttgc gcaccgtaat
     5221 gcgcgaaacg ccggttaact gacttaaatt acgctcaccg ggcaaaatat tgccatgctc
     5281 cagcaccccg ttgcgcacgg cattttttac cgtttcggca aacttaatat aaagcggcgt
     5341 gttatccgcc gctaaaatac gctcgttcag ttgggcaatc agctgggtat gcgcttgttc
     5401 catttgtctt tttcctgccg gggagtctgg taggagtata tcactgcttc atatttaagg
     5461 cgtttcttta atcccatcaa cgaatgtctg caactcctcc accgtgccat taaacacatt
     5521 aaaatccacc ggaccattaa tgccatcgac ctgtccacgg tcggaatgct gccagaagcg
     5581 ccaggccatg ccgtcattgt ccgggcgacg ttgataatag tgagccaccc accacggata
     5641 ttcattgaaa tagcccgcca gattggtgtg ataaaaaacg gctcctgagt aaataattgg
     5701 cttttttccc gtacgttttt cgaccatttt tagccactga cttacccgct tgcgtaattc
     5761 ttttgccgat aattttcccc gttcttctac gtccagcacg gcagggaaat cgccttgtga
     5821 gaaatccacc gtttgcagaa ataatctcgc ctgaactgaa gcagataccg acggggaaaa
     5881 ataatgatac gccccgcgca gcaggccatt ttcgcggctt agttgccagt tacgcgaaaa
     5941 ataggggtcc accagctttt cgccttccgt cgccttaata aaggcaaatt gtaagcgaat
     6001 accgttatcg cgcatttttg ccacccgctg ccagtcgatc cgctcctgcc agcgcgaaac
     6061 atctattccg tgaatggtgt aactggcagg aatgcgaata gcgaaagatt taaccgggcg
     6121 ataaccgtag aagtgaatgt agtctttaat ctgaattgct gtcgaataga aaaagttcac
     6181 cgtctgacgc ggataaattg caacgatgga aattagccca agagcgcata ataaaacggt
     6241 gaattttttt cgactggtga ttctcaactg cattggtttc atccctgaat gtcagcacca
     6301 aaggctgaca ataaccaaag cagctatagt acggtgctca ccaccaggcg tggaagtggt
     6361 gaaccggacc aataccgtga ccaacttcca gcgtgtcggc ctgggctaat gctgatgaaa
     6421 gccagctttt tgcctcctgt acggtgtcag cccagtttgt atggcgcggg cgtagtgcag
     6481 ccaacgccgc agagagcgta caaccagtgc cgtgggtgtt tttggtcata atgcgcggtg
     6541 cggtaaaccg ttgttcaccc tcgcgggtaa acagccagtc cgggctttgc tcatcatcca
     6601 gatgaccacc tttcattagc actgcgccac agcccatcgc caacagcgat cgcccttgtt
     6661 ccagcatttc ctgttcggtg cgcgcgtgtg gcgcgtcgag caaggcggca gcttcgggca
     6721 agtttggcgt tattaatgaa acctgtggca ataatcgact gcgcagcgta gcaaccgccg
     6781 aaggtgaaag cagcgggtcg ccgctttttg ccagcataac ggtgtcgagt accacgtttt
     6841 ggatctgata acgttgcaac cgttctgcca ccgcttcaac aatatcggtt tccgccaaca
     6901 taccgatttt agtggtgtcg attcgcacat cgctgaacac cgaatcgagc tgggcggcga
     6961 caaaatcagg ctcaatgcga tacaccgact gtacgccacg ggtattttgc gccactagtg
     7021 cagtaataac tgagcaacca taagcgccaa gtgccgagaa ggttttaaga tcggcctgaa
     7081 tccccgcacc accactcgga tcagtaccgg caatcgtcag agcgttaatt cgtttcatgc
     7141 ctgcacctcc tgcgccaatt gccagagcgc atcaaggaaa tgtggaacaa aactacctgg
     7201 cccctcgctt ctggcgactg cgcgttctcc ggcttgtttc atccagtgac aggcagatgc
     7261 gatattttcc agcgtatcgc ctggtagcgc acaacaggca gcgacaaccg ccgatagtgc
     7321 acagccagtt cctaccactt tggtcattaa cggatcgcca ccgtgaatgc caatgatacg
     7381 atgtccatcg gtaacataat ccacctcgcc agtgaccacg acgattgcgc cagtttcccg
     7441 tgccagtgtt tgtgcagcgg gtatcgcgtt agctgcggca tcagtggtat ccactccccg
     7501 tccaccatta gcaacgccag ctaatgccat gatttccgaa gcattaccac gtatggcggt
     7561 tggcttatgg gacagaagtt ccacacaaaa acggcggcga taatcgagcg caccaaccgc
     7621 aaccggatca agcgtccagg gcgtttgtga tcgtgttgct tgctcaacgg cagcactcat
     7681 cgcttgcgct cgtggctgcg tcagtgtgcc aacgttaatc agcaacgcac tggcgatagc
     7741 cgcaaactga ctggcttctt cggtttcgat aaccatcgct ggcgatgcac cgagcgccag
     7801 caaggtattg gcagtaaagg tttgcaccac atcgttggtc atacagtgca caagagggga
     7861 atgttggtga aaaaggtgta acgcgtgcgc agattgcgct gaactcagca ggtcgacttg
     7921 catagtttgc tcctgccata acgtgaagaa gcaatgacct ggtggtccgt gacttcccta
     7981 cgctggcatt atccagatca ggtgatacgg gtatttctca gccttcacaa agaagggcac
     8041 cccgagtcgt taggttgcga taataaatcg cagaggagga tggtaatgtc cagcgcacgc
     8101 gttgtaaacg gggaaaaacg gcagttttac aatcgcgtta ataaattatt tgatataaga
     8161 atccagcacc ttcagaacga catccagatc ttcttctcgt tttagctcat ccccctggtg
     8221 aacgatgtgt tccgtcagat gacctttaat cacttcccgc atcagaccgt ttaccgcgcc
     8281 acggatagca gcaatctgtt gtaaaactgc agcgcattca tgcggttcgt cgagcatttt
     8341 cttgagcgcc acgacctggc cctgaatctt actggcacgc gctttcagtt tctgtttatc
     8401 acggattgta tgagacatgg caacacctgg ttaacaagaa tatgaaaaat catagcacta
     8461 ttaatctact ggggggtagt atcaggtact gggggggagt agaatcagat tgccgaatta
     8521 atactaagaa ttattatcat gaccgaattt acaacacttc ttcagcaagg aaacgcctgg
     8581 ttcttcatcc ccagcgccat cttacttggt gcgcttcatg gcctggaacc ggggcactca
     8641 aaaacgatga tggcggcgtt tatcatcgcc atcaaaggca ccattaaaca ggcggtgatg
     8701 ctcggactgg cagcaactat ttcgcatacc gcagtggtct ggttaattgc ctttggcggg
     8761 atggtgatca gcaaacgctt tactgctcaa tcagcagaac cgtggctcca gttgatttcc
     8821 gcagtgatca ttattagcac cgcgttctgg atgttctggc gtacctggcg cggcgaacgc
     8881 aactggctgg agaatatgca cgagcatgat catgagcatc atcaccacga tcacgaagat
     8941 caccacgacc atggacatca tcaccatcac gaacatggcg agtatcagga tgcccatgca
     9001 cgagcccatg ccaatgacat taaacgacgc tttgatggta gagaggtcac caactggcaa
     9061 attttgctat ttggcttaac cggtggcctt atcccctgcc cggcagcaat taccgtgctg
     9121 ttgatttgca ttcagttgaa agccctgaca ctgggcgcaa cactggtcgt cagtttcagc
     9181 ctcggcctgg cgttaacgct tgtcaccgta ggcgttggcg cagcaatcag cgttcagcag
     9241 gtcgcaaaac gctggagcgg atttaacact ctcgctaaac gcgctcccta tttttccagt
     9301 ctgttgattg gcttagttgg tgtgtatatg ggcgtacatg gcttcatggg cataatgcga
     9361 taactcaatc ctttaggctt gcatgttatc ttcagcccag gatgattctt aaatcagcta
     9421 tttccgctga ctctctgctc gctaaggatg cttttagggc atcctttcat ttacactttt
     9481 tacgaaatca tgggatcact aacaaaatat cgcttgtcag ttatattgta tggcaggaaa
     9541 gatatgcgac tgatattaca gatccccaaa gtggagagtt tatgaccatt aaaaataaga
     9601 tgttgctggg tgcgcttttg ctggttacca gtgccgcctg ggccgcacca gccaccgcgg
     9661 gttcgaccaa tacctcggga atttctaagt atgagttaag cagtttcatt gctgacttta
     9721 agcatttcaa accaggtgac accgtcccag agatgtaccg caccgatgag tacaacatta
     9781 agcagtggca gttacgtaac ctgcccgcgc ctgatgccgg gacgcactgg acctatatgg
     9841 gtggcgcgta cgtgttgatc agcgacaccg acggtaaaat cattaaagcc tacgacggtg
     9901 agatttttta tcatcgctaa aaaaaagccc cctcggagag aggggggaat gcagacacct
     9961 taatattttt attattagcc acttgctcgt cttgcttgtt attagtcgta tttcacgttg
    10021 attaatgcgg ttgcctccag tgcgccagat ttaactttgt ttgtatcgta gacgtagtaa
    10081 ctggccgtta tcggaattga aaccgttgta tctgtaagtt ttgactttgc cgcaatttct
    10141 tttaaatttg tatatccttt actgctgtta gtgatattta aaagtgtgac tggcgtacca
    10201 ttactttcaa gaataaatcc tacgccatta tcttccgcac ctttcaggat cgtgttgctg
    10261 ttggttaaca agtcagaact tgataaatac acctgcattt tccgtgatgt ttgattttgt
    10321 tgacctaata aatttttaca acgcagaata atatcccctt ttttactatt tattgttgtt
    10381 ttgttacctg tggctggtaa ttgtgaaaca ggaatactct ctaacgtaat taataaatcg
    10441 tccggtttgc aggttgtggt tatcgggttg tatgacaatg tcagattaaa ggaatagaca
    10501 cctgcatttt gtgccaatgc atttctgtag ctatttacac atgcatccca tgacttacca
    10561 cctaataaac acagtagaaa ttgtactgcg gcactcccgc gtgtcgatga tgaagcactg
    10621 gataatgtcg caactccatt aatcatcact gaaccaccgc cccccgcaga aacattcact
    10681 tttgcattac tggccgcagg tgtcaccgtt accgaataac cactggtgaa actggtctga
    10741 ttatttgaca acgtaatgtt gtttttattt gtccattcaa aatgtaaatt taatttaatg
    10801 gtatcgttat aaagtccaac aataatatcc gacgggctca taggggtgat agtatttgta
    10861 ctcacgcatg acgaatcatt aaaattatgc tcatatgtcg ccatttgtat ttgagtaagt
    10921 gcaaaatccc cctgaacaac caaattgtgc tgaacagata actcactata acatgccgca
    10981 tagctcacag gcataaccat catcattact ataaatagca ttatgcgaat ttccatagaa
    11041 ccacctgact attactggca aatataattt ttactttcat caatctcttt accgaaggtg
    11101 attgtgcatg aaagcccttg ttgcttatca attgccacat taaccgatgg cggtacttca
    11161 ttggtgcgaa taaataactg gcttccctgg ccgacaacac caatattatg accatggata
    11221 tcattgactt cataaccaaa cgttaatggt tgcccatccg ctcttaatgc ttttataaac
    11281 catggcttgc gctgatcggt atcaaaatta accagtacaa ccgcgccgcg ataaggggcg
    11341 gcaattttcc ggttgccacg taattctgct tcgctatcgc tttgtgacac atccaacatc
    11401 aggtaatttt cccgataagg tgtcattccg tcgtatacca ccactccatt acggtttgtt
    11461 gtgcgatatt tttgcccatt gacataagca tctttaattc ctggcgcatt catcacagca
    11521 aacgtttcag aaagacggtt tgccagatta acgccacccg accaggcgac aatgccccct
    11581 gaaacactgg ctccagcctg tcgataagca ctcgactgac tataactgcc attcactgtc
    11641 gcaaccggcg cgttccaggt taaattcacc ccagctgtcg tttcatttcc ctgatactga
    11701 taactcaggt tgaccccata gttaaactga tcccggcttc caacggttcc tgataatccc
    11761 gtattatttg aggcaaaccc ctgatcatca aacgtcgttg agttagacat atatatttgc
    11821 cgacgaggcg tcgtaacgtc atcaccccaa tcaaagggaa tcgatataaa aatattaaaa
    11881 cgtttctctt catgatgatt ctcgtcataa gcatggcttg ccgcgagggt atagcttatc
    11941 cttcgcaggt tgttggaata actcaactga taatccttac tgctgccgct acgcccccag
    12001 taatctcgcc ataacgtact taaggacaca gaaccccagc cttctggcaa tgactgactc
    12061 atattggcag aaaagctatt tttacggcca aaatcgttct ggtaataatc ggcaatgtca
    12121 tagatatcgt tttcatcacg gcgataatta tctttattgt ttgcccatac gtgatcatta
    12181 aatgtccggt aatcacgcga cgaataacgc caggccgcca gaccaaaacg tgtcgacgtt
    12241 tggctcacaa atttgttgta ggcaatttga taactttgcc cgtcaaacac atcgccgttg
    12301 tcttgtatac tatgcgactt cgtggcatcg acggaaatgg caccaatgcg tgtattccag
    12361 ccagtcccca aagtgaacgc gtaataatta ttcgcgacca tcgatccgcc atacagcgtc
    12421 aataagttat taaaaccata ctgataaccc gcctgaacaa aatcactttg tttgctcgcc
    12481 ccttcaatat ggctacgacc tgccgcaaaa tcatatttcg acacgccggg ttgcagcata
    12541 ttcggcactg ctgcataagg caccagatag gtggttaccg aaccgtccgc ctctttcacg
    12601 ctgacatcaa gatctgctcc acctccggcc aactgcaaat ctgtaatcgc aaacgggcca
    12661 ggaggaacct ctttctgata aaccacaaaa ccattctgtt caatagttac cagcgcgtta
    12721 ctctgagcaa tcccctgcac ccgtggcgta aaattttgtt tcgagttagg caacatctgc
    12781 atatcacgaa acaaccgcac accgctgaag cgaacagaat caaaaatatc gcttgatgtg
    12841 tacatatcac ccacacgaag cgtgccgaga agttgggcaa atccacgttc cagatacagg
    12901 gtattgcttt tccacacccc tggattgtta tttgttttac tgaaactggc atcagaatgc
    12961 agttgccacc cctgtaaatt taacccgctg ttaaaacgta catatgtact cttgctatta
    13021 ccggacgctt tataatcgct gtaatactga ctcacataat aagaggtata aaacgcatta
    13081 ataccccgct cccagttttc tggtggaaca tagccacttt ccagatcttc cacccaggcc
    13141 tgcgggacac tgaaatcgag acgaaaaact ccgatatccc aggtatagct cccgccctga
    13201 ataagttgct taaatgttaa acattgctta ccgctggcaa agctatcggt attaatgcct
    13261 aaccgcttga tcatttccct tgataaacat gtttcttgcg ggttgtcttt aacaatgatc
    13321 tcatatttcc cacgccattg cttattgaca taaatatcga tgtcatacgg ccccggcaag
    13381 ggctgattat cttcaagacg aatatttgca acctgctggt ctttcattcc acctatcatg
    13441 aaatgggtat caaaggtttc ttcagctgca taagcttcaa tgccgatcaa taacgctacg
    13501 attgctgatg caagtggggt cattctcaac ataggcagct cctgcaatta aattttgtca
    13561 ctaatataat tgccatggtc atcgataatg gtcagatgcc agttatttgc attattactt
    13621 ttgacattaa cactctgact ttctaagggg gcaatcataa tagtttcata attgactttg
    13681 acattattag ctttgacatc cgaaatcgtc acccaattag ctgagtcatt ttttatcacc
    13741 aatccattgc cactgcgatt tatcagtaac tttttaaagg tcactttatt taccggagca
    13801 atacccgctg gccggtaaaa caacttaatt ctgttttgca tcgcaaactt cagtgcattc
    13861 ttaccttctt gttctggact atttggtgga atatccagaa cattcagata aaaaatactc
    13921 tctttattag tgggtagttt attcggcata attttaattt ttacttgctg cccggaattt
    13981 gcccctattt ttgccactgg tggcgttaac atgaaaggaa cctgaatttt ttctggtggt
    14041 aatgacgtat cgccatcatc aatccacgcc tgcatcagcg aagaacggtt tccctggttc
    14101 atcaactgca ccatcacttc tttgtgctct gccgggtaaa taatacgcgt cccgtagata
    14161 acaataccgg catgtgcagg aaggaccata gaagaaatga gtaaagctaa taatcctttc
    14221 atcttaatgc ctcttaaatt aaaaggccgc catggagata gcggccatgt taattacctg
    14281 aagaatcttg caattttatt tattaatcct gcgccacacg cataatcagg gaagataaca
    14341 catcacctga attcactgag ttatctgtca gtttcaggta ttttgcttta aacggaatgg
    14401 catattgacc gtccgctgtt ttataactct cgatgttgaa aggtgcagaa acatccagga
    14461 catcgctgtt atcacctaaa tagagataat aacccacggt atcgttgcta ctggctatcg
    14521 ccttatcgtt tccggttatt ttatcgttgg taatcaacga gagttttaca ccagcaactt
    14581 ctttcggaca gctaattttt aacatgctgc tggttacacc gactgcacca tcataggtcg
    14641 agtatttagc tttaccttca aagaccgtag ttggtacagt tggcatatta atacttactg
    14701 aatcgccacc tgcttcgaat ttacacggag attctgcaat attcccttta atggttaatg
    14761 ttccggtatc aacgtctgcg gcaaataccc cagcgctcat aaaaaaagaa gaaaaaacag
    14821 cggcagcaat aattgaacgt ttcataatat gctccttaat aaaatctatt tttggttgtt
    14881 aagagtctgt atctcttgga aacaaatttc tcacaccaaa aaagttgccg caatgtaaac
    14941 acattgtttt attgat
//
LOCUS       DAZFJL010000118        14516 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFJL010000118 DAZFJL010000000
VERSION     DAZFJL010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.118"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..1077
                     /gene="emrK"
                     /locus_tag="ACHJ8C_003565"
     CDS             <1..1077
                     /gene="emrK"
                     /locus_tag="ACHJ8C_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO5553356.1"
                     /translation="GAYAYWSMELKDMISTDDAYVTGNADPISAQVSGSVTVVNHKDT
                     NYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARIQYQQ
                     SLEDYNRRVPLAKQGVISKEALEHTKDTLISSKAALNAAIQAYKANKALVMNTPLNRQ
                     PQVIEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVVPARQMWV
                     NANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNATGNW
                     IKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIAEMPDLASTVTSMPAYTS
                     KALVIDTSPIEKEISNIISHNGQL"
     gene            1077..2615
                     /gene="emrY"
                     /locus_tag="ACHJ8C_003566"
     CDS             1077..2615
                     /gene="emrY"
                     /locus_tag="ACHJ8C_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO5553357.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIILLTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2723..4051)
                     /gene="dsdA"
                     /locus_tag="ACHJ8C_003567"
     CDS             complement(2723..4051)
                     /gene="dsdA"
                     /locus_tag="ACHJ8C_003567"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO5553358.1"
                     /translation="MENAKMNSLIAQYPLVEDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKVFPETAAAGGIIESELVAIPAMQKRLEKEYHQPIAGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVNADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4069..5052)
                     /gene="dsdX"
                     /locus_tag="ACHJ8C_003568"
                     /pseudo
     CDS             complement(4069..5052)
                     /gene="dsdX"
                     /locus_tag="ACHJ8C_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            4973..5761
                     /gene="dsdC"
                     /locus_tag="ACHJ8C_003569"
     CDS             4973..5761
                     /gene="dsdC"
                     /locus_tag="ACHJ8C_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DsdC"
                     /protein_id="HFO5553359.1"
                     /translation="MASFNNEVLVFLAIEKARGINSTMPTSRVYWALKSSLDTLNQEI
                     LDIKNQELSGTLTLYSRPSIAQCWLVPALGDFTRRYPSISLTVLTGNDNVNLQRAGID
                     LAIYFDDAPSAQLAHHFLMDEEILPVCSPEYAHRHDLTNTVINLRHCTLLHDRQAWSN
                     DSGTDEWHSWAQHYAVNLPTSSGIGFDRSDLAVIAAMNHIGVAMGRKRLVQKRLASGE
                     LVAPFGDMTVKCHQHYYITTLPGRQWPKIEAFITWLREQVKTTS"
     gene            complement(5861..5935)
                     /locus_tag="ACHJ8C_003570"
     tRNA            complement(5861..5935)
                     /locus_tag="ACHJ8C_003570"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5900..5902),aa:Arg,seq:cct)
     gene            complement(6011..6943)
                     /gene="yfdC"
                     /locus_tag="ACHJ8C_003571"
     CDS             complement(6011..6943)
                     /gene="yfdC"
                     /locus_tag="ACHJ8C_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO5553360.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSVLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGIVLLGNIIGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            7237..7992
                     /gene="mlaA"
                     /locus_tag="ACHJ8C_003572"
     CDS             7237..7992
                     /gene="mlaA"
                     /locus_tag="ACHJ8C_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO5553361.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADSLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(8055..9395)
                     /gene="fadL"
                     /locus_tag="ACHJ8C_003573"
     CDS             complement(8055..9395)
                     /gene="fadL"
                     /locus_tag="ACHJ8C_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO5553362.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTQQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVAPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            9767..10051
                     /gene="yfcZ"
                     /locus_tag="ACHJ8C_003574"
     CDS             9767..10051
                     /gene="yfcZ"
                     /locus_tag="ACHJ8C_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO5553363.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            10232..11542
                     /gene="fadI"
                     /locus_tag="ACHJ8C_003575"
     CDS             10232..11542
                     /gene="fadI"
                     /locus_tag="ACHJ8C_003575"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO5553364.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARTEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHHSHQRAAQAWS
                     EGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLFDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDESKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            11542..13686
                     /gene="fadJ"
                     /locus_tag="ACHJ8C_003576"
     CDS             11542..13686
                     /gene="fadJ"
                     /locus_tag="ACHJ8C_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO5553365.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEVLARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQAVKLGLVDDVVPHSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRNIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPRGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYCGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCATHTIFASNTSSLPIGDIAAHAARPQQVIGLHFFSP
                     VEKMPLVEIIPHASTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIMPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGCKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCDRLVEMSERGESFWKTTATDLQ"
     gene            13889..14374
                     /gene="sixA"
                     /locus_tag="ACHJ8C_003577"
     CDS             13889..14374
                     /gene="sixA"
                     /locus_tag="ACHJ8C_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO5553366.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVGAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGQFNWQMSPCNLKMAKA
                     I"
BASE COUNT         3694 a         3423 c         3641 g         3758 t
ORIGIN      
        1 ggtgcctatg cctattggtc aatggaatta aaagacatga ttagtacaga tgacgcctat
       61 gtcacgggaa atgcagatcc aatttctgca caagtctcag gtagtgtcac tgtcgttaat
      121 cataaagata cgaactacgt tcgacaaggt gacattttag tttcactgga taaaactgat
      181 gccactatcg cactcaataa agctaaaaat aatctggcaa atattgttcg gcaaacgaat
      241 aaactatact tacaggataa acaatacagt gccgaagtcg cttcagcacg tattcagtat
      301 caacaatctt tagaagatta taaccgtcgt gtgcctttag cgaagcaagg ggttatttca
      361 aaagaagcgc tggagcatac caaagatacg ttaataagta gcaaagcggc attgaatgct
      421 gctatccagg cttataaagc gaataaagct ttagtaatga acacaccatt aaaccgtcag
      481 ccacaagtca ttgaagctgc agatgcaacc aaagaagcct ggttggcgct taaacgtacg
      541 gatattaaga gtccggttac cggttatatt gcccaaagaa gtgttcaggt cggcgaaaca
      601 gtgagtcccg gacaatcgtt aatggctgtc gtaccggcgc gtcaaatgtg ggttaatgcc
      661 aactttaaag aaacacaact cacggatgta cggattggtc aatcggtcaa tattatcagc
      721 gatctttatg gtgaaaatgt tgtgtttcat ggtcgggtga cagggatcaa tatgggaacc
      781 ggcaatgcgt tctccttatt acctgcacaa aatgcgacag ggaactggat caaaatcgtt
      841 cagcgtgtac cggttgaagt ttctctggat ccaaaagaac tcatggaaca ccctttgcgc
      901 attggtttat cgatgactgc aactattgat acgaagaatg aagacattgc cgagatgccc
      961 gatctggctt caaccgtgac gtccatgcct gcttatacca gtaaggcttt agttatcgat
     1021 accagtccga tagaaaaaga aattagcaac attatttcgc ataatggaca actttaatgg
     1081 caatcactaa atcaactcct gcaccattaa ccggtgggac gttatggtgc gtcactattg
     1141 cattgtcatt agcgacgttt atgcaaatgt tggattccac tatttctaac gtcgcaatac
     1201 cgacaatatc tggctttctg ggagcatcaa cagacgaagg cacctgggtt atcacctcgt
     1261 ttggcgtagc aaatgccatt gcgatccctg ttaccggcag gctggcacaa agaataggcg
     1321 aattaaggtt atttttactt tcagtaacct ttttttcgct gtcttcatta atgtgtagtt
     1381 tatcgaccaa tctagatgtg ctgatatttt ttagagtcgt tcaggggtta atggcggggc
     1441 cgttaattcc actgtcacag agtttattat taaggaatta tccaccagaa aaaagaacat
     1501 ttgctctggc attatggtca atgaccgtga ttatcgctcc gatatgtggg ccgatattgg
     1561 gcggctatat ttgcgataac tttagctggg gttggatatt tttaatcaat gtccctatgg
     1621 ggattatcgt cctgacatta tgcttaacct tacttaaagg acgagaaact gagacttcac
     1681 cggtcaaaat gaatctacca ggactgaccc tgttagtgct cggtgttggt ggcttgcaaa
     1741 ttatgcttga taaagggcgc gatctggatt ggttcaactc gagtacaata atactactaa
     1801 cagtagtatc agttatttct ctgatctctt tagtcatttg ggagtcgacc tcagagaatc
     1861 cgattcttga cctcagtttg tttaagtccc gtaatttcac cattggtatt gtgagtatca
     1921 catgcgcgta tttattttac tctggagcga tagttcttat gccgcagtta ctccaggaaa
     1981 cgatggggta taatgcgata tgggccggac ttgcctatgc gcccatcggt atcatgccgc
     2041 tattaatatc acctttgata ggacgttatg gcaacaaaat tgatatgcgg ttgttggtga
     2101 catttagttt tttgatgtat gcggtttgct attactggcg ttctgtgaca tttatgccaa
     2161 cgattgattt tacaggcatc attttgccac agttttttca gggattcgcc gttgcctgtt
     2221 tctttttacc cttaacaacg atttcgtttt caggcttgcc agataataaa tttgccaatg
     2281 cctcgagtat gagtaatttt tttcgcacct tgtcaggatc agttggtacg tcgttgacaa
     2341 tgacactgtg gggacgccgc gaatcgttac accatagtca gttgacagca accatcgatc
     2401 aatttaaccc cgtgtttaat tcatcgtcac aaattatgga taaatactat ggttcgcttt
     2461 caggagttct taatgaaatt aataatgaaa taactcagca gtcactttct atttctgcaa
     2521 atgagatttt ccgtatggcg gctattgctt ttatcttact tacggttttg gtttggtttg
     2581 cgaaaccgcc gtttacagcg aaaggcgttg ggtgatggaa aaagaggagg ctatagcagg
     2641 taaaacggca gaatcttaag tctgcgtcat ccgcagcatc gctcacccag ggaaaggatg
     2701 gcgatgctgc gttgaaacgt tattaacggc cttttgccag atattgattc atctcttctt
     2761 ccggcaccat tccacctccc gtcgcccaca ccagatgagt ggcattacgc agttgttctg
     2821 cgctgaaacc gtgcatctgt tggtaactta ctgatgcgca cacgcgctga ggcccggcca
     2881 tacccgccag tgccgaaggt tcaagacgaa tgccttcttc ctgcgccagc cagccaagca
     2941 tgtcatacat ggtttgatcg ctaagggtat agaagccatc cagcagacgc tccatcgccc
     3001 gcccgacaaa gcccgatgcg cgaccaaccg caaggccatc ggcagcggta aggttgtcga
     3061 tgccaatatc ctgaacagaa atctgatcgt gtaatccggt atggacgcct aataacatac
     3121 acggggagtg cgttggttcg gcaaaaaagc agtgaacatg atcgccaaac gccagtttaa
     3181 gtccgaatgc gacgccacca ggaccaccgc caacaccaca cggcagatag acaaacagag
     3241 ggttatcagc attgacgatg cgaccttgct gagcaaattg tgccttaaga cgctggccag
     3301 cgacggaata tccaaggaac aacgtgcggg aattctcgtc atcaataaag aaacagttcg
     3361 ggtcagactg cgctgcttta cgtccttcct cgacagcaac accataatct tgctcgtatt
     3421 ccacgacagt aacgccatga ctgcgcagtt tcgctttttt ccaggcgcgg gcatcagcag
     3481 acatatgcac cgttacttta aagccgatgc gggcgctcat aataccaatt gataacccca
     3541 gatttccggt tgatcccaca gcaatactgt attggctaaa gaactgttta aactccgggg
     3601 aaagcagttt gctgtaatca tcctcaagtg tcagcaaacc cgcttccaga gccagttttt
     3661 cagcatgggc caggacttca taaatcccgc cacgtgcttt tatggagccg gaaatcggca
     3721 aatggctgtc tttttttagt agcaattgcc cggcgatcgg ctggtgatat tccttttcca
     3781 gccgtttttg catagcagga atagcaacca gttctgattc aataatcccc cccgcggcag
     3841 ccgtttcagg aaatactttt gccagatacg gcgcaaaacg agacaggcgc gcatgggcgt
     3901 cctgaacatc ctgttcggtc aggccaacat aaggtaaacc ttctgccaat gaggtagtgc
     3961 caggattaaa ccaggtggtt tctttaagag caaccagatc ctctaccaaa ggatactggg
     4021 cgatgagcga attcatttta gcgttttcca tagtacgtct ctttgcgctt agatgataaa
     4081 tgacagcagg aatgtgcccg ccagagcgac gactgaagcg ataaatgttg ctgtcgtata
     4141 gtatttaaat gtttcattga gcgtagcgcc gcaatattgc ttcaccagcc agaaaagcga
     4201 gtccgtaacg atcgtgcagc caatcgcacc tgaaccgatg gcaattgcaa taatttccgg
     4261 gctgatgtcg ggatacagcg gcagcatggg tgcaacaatt gccgttgccc ccatcattgc
     4321 caccgtagcg gagcccactg cagcatgcag aataagtgcc accaaccagg ccagaagaat
     4381 cgggtgcata tgcatattgg agagaataac tgccagcgta tcagcgagac tgctgctttt
     4441 taaaatggcg ttgaatgcgc ctccggcccc gataatcagc aaaatattag caatagaacc
     4501 gaaaccattt tccgtatgtg tgagcatcgt ccccatgctc atatgctgac gtatacctaa
     4561 cacataatag gcgacaaata cggcgataaa catggcagtg atagggttgc caataaactc
     4621 aagcaaggta tacaaaccac tctcacgcgc catattcaat tcggcaatcg ttttaaccaa
     4681 catcagcgca atgggtagca gtatggtgaa taacgttgcg cctaatgacg gtagtgtttt
     4741 ttcatcgcga actttgagat cggcaaactc tgtgggtaca ggtttaaagg gcagtcgttg
     4801 ccccagaaat ttaaggaaaa gtgggccacc gatcagtgat gccatcagcc caaccagcaa
     4861 accgtagacg atcaccgaac cgatatctgc gcccagctta ttggcaacat ataaagcagc
     4921 cggatgtggg ggaaccacgc agtgcactgc catcaatgcg gtacacagcg gaatggccag
     4981 ctttaacaat gaggtattgg tttttttggc aattgaaaaa gccagaggaa tcaatagcac
     5041 gatgcccact tcacgtgttt attgggcgct aaaatcgtcg ctggataccc tgaatcagga
     5101 aattctggat atcaaaaatc aggagttatc gggaacgtta acgctgtatt cccggccctc
     5161 tatcgcccaa tgctggttgg tgcccgcatt aggtgacttt acacgccgat atccgtctat
     5221 ttcgctcacc gtgctcactg gtaatgacaa tgtcaatttg caacgtgccg gaatcgattt
     5281 ggcgatctac tttgatgatg cgccgtcagc gcaattggct catcactttc tgatggatga
     5341 agaaatcctg ccagtttgca gcccggagta cgctcataga catgatttaa ccaacacggt
     5401 aattaacctg cgtcactgta cgttgctcca tgacagacag gcatggagca acgactccgg
     5461 tacggatgaa tggcatagtt gggcgcaaca ttatgcggtt aatttgccaa catcttctgg
     5521 aattggcttt gatcgctctg atttagccgt tatcgccgcg atgaatcata ttggggtggc
     5581 gatgggaagg aaacgcctgg tacaaaaaag gcttgccagt ggtgagctcg tcgcgccgtt
     5641 tggcgatatg acggtgaaat gccatcagca ttattacatc accacattac cgggcaggca
     5701 gtggccaaaa attgaggcat ttattacttg gttaagagaa caggtaaaaa ccacttcata
     5761 atgaataaga ccacgagttc ctggtgtgcc ttattcggca ttgctagtgc gaatttaata
     5821 atttacggtt tttacgtggt tttagggata aacacaagaa tggtgtcccc tgcaggaatc
     5881 gaacctgcaa ttagccctta ggaggggctc gttatatcca tttaactaag aggacaatgc
     5941 ggcacgagta tacccgctaa tggactacgg agtaagtaca cagccgctcg attgcttaaa
     6001 ccctcgccat ttatgccggg tttttatcat ttttcttaat gttttccgca cgttctgctt
     6061 tttggcgtgc ttctgctttg cgcttgttgc tcatgtcgtt acgaatctgt gcatgactca
     6121 ttaacgcgaa gataaaggtg ccgccgcaga tgttccccgc taaagtaggt agtgcaaagg
     6181 gccagatgaa atcgctccag tgcagcgtgc cgttaaacac cagatagagg atttcaacag
     6241 aaccgacaac gatgtgggtg gtgtcaccca gggcaataag ccaggtcatc aatataatca
     6301 ccacaatctt tgccgcaccc gctgcaggaa acatccatac catagtggcg atcagccagc
     6361 cggaaatgat cgcgttggca aacatctcgc tgggggtgtt cttcatcaca tccatgccga
     6421 ttttgacaaa tgcatcgcga gtttcttcat tgaagatagg catatattca aatgcccacg
     6481 cagcaatacc tgtcccgata atattaccca gcagcacgat gccccataac cgcataagta
     6541 agccgacgtt actcattgtc ggtttttgca tgacgggtag taccgcagtt acggtgtttt
     6601 cggtaaataa ttgctggcgg gccattatga cgataataaa accaaaggta taaccgagat
     6661 tctccagcaa gacgctgccc ggcacgcctt ccagttcgac atgaaatatc ccttttgcca
     6721 gtagcgaagc tcccatcgac aggcccgccg caatggctga ccacagtagc gccattgcgt
     6781 cgcgttccag ctctttttca ccatcctggc ggatatgctc atgaattgcc atcgcccggg
     6841 aggggagtcg gtcttcatct atttctattt ttttaccgcg ctctttttct tcgctctcaa
     6901 cttcaatttc gtcgctgtgt tgatcaattt tgtcgttgtc catggtctct tctcttgaat
     6961 tagcacgtat agctaaagcg tagcggcttt tttgctcgca actggcggga gttactctga
     7021 aaatgtagaa aaggccgcgt ttgccttttt ctgtttctat tgaatcaagt agcctacagg
     7081 gcggcgatta ccaggctatg atcaaatcag caaatcaggg cgtctggaca tcagttgacg
     7141 tgctgttaca atcgcccaca cctaaacagg cggatacggt atcgttccgt catggatggc
     7201 aaactgcata agccataaaa aaacagggag acatttatga agcttcgcct gtcggcgctt
     7261 gctctgggaa ctacgcttct ggtggggtgt gcgagttccg gtacagatca gcaagggcgt
     7321 tctgacccgt tagaagggtt taaccgcact atgtacaact tcaacttcaa tgtattagac
     7381 ccgtatattg ttcgaccggt cgctgtcgcc tggcgtgatt atgttccgca acctgcgcgt
     7441 aacggtttga gcaactttac cggcaacctt gaagaacctg cggtgatggt taactacttc
     7501 ttgcagggcg acccttatca ggggatggtc cactttaccc gctttttcct gaataccatt
     7561 ttgggaatgg gcggctttat tgatgttgca gggatggcga acccgaaact gcaacggact
     7621 gaacctcacc gcttcggtag tacgcttggt cattatggtg tgggttatgg gccgtacgtt
     7681 cagttaccgt tctacggtag cttcacgctg cgtgatgacg gtggtgatat ggcggatagt
     7741 ctttacccgg ttctttcctg gctgacctgg ccgatgtctg tgggtaaatg gacgcttgaa
     7801 gggatagaaa ctcgtgcgca gttgttggat tccgacggtc tgctgcgtca gtcctccgat
     7861 ccttatatta tggtgcgtga agcgtacttc cagcgtcatg atttcatcgc taatggcggt
     7921 gaactcaaac cgcaggaaaa tccgaacgcg caagcgattc aggatgattt aaaagatatc
     7981 gattctgaat aagaaacaaa caaaaaaggt gagtcgcaaa actcaccttt tttcttaatt
     8041 aagctaacgc gttatcagaa cgcgtagtta aagttagtac cgaacagcca ggctttacct
     8101 tcagactcga actgatatgg gccttcgtta attttcacgc tctgaccgtg catataagaa
     8161 acaccaacgt cgactgaagc atctttatta aatgcgtaag tcgtacctgc actcagccag
     8221 aaacggtcct ggtccggaat ggagatagaa cgattctgtg ccggaactgg gctgtcatca
     8281 aaggcgatac cggtacggaa ggtccagtta tcatcgtagt aataagtggt acccaacgcg
     8341 atgcggtaag catctttaaa gccttcatgt ttctggaaca gcgtgtcgcc actcgttgag
     8401 gtcgctttca gctgctggaa ctgactccag ctggtgtaag ccaggctata gtgaattgcc
     8461 cactgcggag caacacggtt ataacccgac acttcccaca tttcaggcag gttcagcgtc
     8521 agataacctg actgcgttgc gccacctgtc gcggtaggaa ttggcaaacc gtagttatta
     8581 aatgcacgat taagatcgct gctgtagtta cctttgaagt caattttcac ttcagaacgg
     8641 taggtcagtg catagcggtt atttttatcc agttcataca ggattccggc gttccagcca
     8701 aaaccccact ggttaccgtt cagatgagcg attttggtat tactgtcgat accgttggcg
     8761 gtagctgcca atgcttgccc ttgctgagtt tgtccagcag gagattgcat aatctgaccg
     8821 gcaaccagct gccccagatc gcctgcgaaa cgttcaattt tcgcgcgagc gtagacggcg
     8881 ttgaaaccaa gaccaaagct ccatgcatta tttaagcgat acgcaccgct taagttcagg
     8941 ttcatggttt caaggtcggt tgtaccaccg acagaaccgc ctgcataagt atcgttaaac
     9001 tctgttgcca gaccatagtt agaggtaata gaagcgcccc aaccaaattg gtcgttaatc
     9061 ggtgcaacaa agtgcatgtt cggaacccat gccgtaggcg cgatgttatc ggctttcagg
     9121 ctacgaccag acggagacgt tccgctgata tttacatccg gatcaatata aaccgcaccc
     9181 gcagaaaatg tcgggcggtc aaacatggta atcaatgcgg ggttacggct aacgttacct
     9241 gcatcatcgg caattgcacc ttcccctgaa taagcccggc ccaggccaga ggaagaaaat
     9301 tcgtttaact gaaagcctgc cgaccaggcc tgggtggaga taagtgccac tgcgactgcg
     9361 agagcagact ttgtaaacag ggttttctgg ctcatgacca taacctcaat gatttatttt
     9421 tatacaaact atgttacgtg ctgtaacagg agcgcgaagt gtagggtctg aggtacatct
     9481 tagaaatcag accagtggcg agagtatagg tcggaccagc tggaatgttg caagtatgtt
     9541 tctatctttt ttcagatatg atctaagaaa cgcgatccag ctggcaaaat tgggaggtgg
     9601 cgcactcatg gattcgggtg aattttgttt tagatcattt ttaagtgtga tttcggtcac
     9661 ttatccgatt ttgcagaatt aacaactgga gcagcacttt ccggaagcgt aaaatatcca
     9721 caacgtttat ctggcacctt gggtgcacat acagaggaaa tcaacgatga gtaaatgtag
     9781 tgctgatgaa accccggttt gctgctgtat ggatgttggc accattatgg acaactccga
     9841 ctgcaccgcg tcttacagcc gtgtgtttgc aaaccgcgca gaagcagaac aaacgctggc
     9901 ggcgctgact gaaaaagccc gtagcgtgga atccgaaccg tgcaaaatca ccccgacttt
     9961 cactgaagag tctgacggtg tgcgcctgga tatcgatttc acttttgcct gcgaagctga
    10021 aatgctgatc ttccagctgg gtctgcgtta attcctctcg ggtacatacg cccctgtcag
    10081 ttgctggcag gggcgttttc ttttctcttc ttcttttctc tctgtgtcat agctcccact
    10141 ttcccttttc tccacttggc taaatgtaaa aaaatggtta agactatgat caggtcagac
    10201 cactttattt atttttttac aggggagtgt tatgggtcag gttttaccgc tggttacccg
    10261 ccagggcgat cgtatcgcca ttgttagcgg tttacgtacg ccttttgccc gacaggcgac
    10321 ggcttttcat ggcattcccg cggttgattt agggaagatg gtggtaggcg aactgctggc
    10381 acgcaccgag atccccgctg aagtgattga acaactggtc ttcggtcagg tcgttcagat
    10441 gcctgaagcc cccaacattg cgcgtgaaat tgttctcggt actggaatga atgtgcatac
    10501 cgatgcttac agcgttagcc gcgcttgcgc taccagtttc caggcggttg caaacgtcgc
    10561 agaaagcctg atggcgggaa ctattcgcgc ggggattgcc ggtggggcag attcctcttc
    10621 cgtattgcca attggcgtca gtaaaaagct ggcgcgcgta ctggttgatg tcaacaaagc
    10681 ccgcacaatg agccagcgcc tgaaactttt ttctcgctta cgtttgcgtg acttaatgcc
    10741 ggtgcctccg gcagtggcgg aatactccac tggattacgg atgggggaca ccgcagagca
    10801 aatggcgaaa acctacggca tcacccgaga acagcaagat gcactggcgc accattcgca
    10861 tcagcgtgcc gctcaggcgt ggtcagaagg taaactcaaa gaagaggtga tgactgcctt
    10921 tatcccccct tataaacaac cgcttgtcga agacaacaat attcgcggta attcctctct
    10981 cgctgattac gcaaagctgc gtccggcgtt tgatcgtaaa cacgggaccg taacggcggc
    11041 gaacagtacg ccgctgaccg atggcgcggc agcggtgatc ctgatgactg aatcccgggc
    11101 gaaagaatta gggctggtac ctctggggta tctgcgcagc tacgcattta ctgcgataga
    11161 tgtctggcag gacatgttgc tcggtccagc ctggtcaaca ccgctggcgc tggaacgtgc
    11221 cggattgacg atgagcgatc tgacattgtt cgatatgcac gaagcctttg ccgcccagac
    11281 gctggccaat attcagttgc tgggtagcga acgttttgct cgcgacgtac tggggcgtgc
    11341 acatgccact ggcgaagtgg acgagagcaa atttaacgtg cttggcggtt cgattgctta
    11401 cgggcatccc ttcgcagcga ccggcgcgcg gatgattacc cagacattgc atgaacttcg
    11461 ccgtcgtggc ggtggatttg gtttagtgac cgcctgtgct gcgggtgggc ttggcgcagc
    11521 aatggttctg gaggcggaat aatggaaatg gcatcagcgt ttacccttaa tgttcgtctg
    11581 gacaacattg ctatcatcac catcgacgta ccgggtgaga aaatgaatac cctgaaggcg
    11641 gaatttgcct cgcaggtacg cgccattatt aagcaactcc gtgaaaacaa agagttgcgt
    11701 ggcgtggtgt ttgtctccgc gaaaccagac aacttcattg ctggcgcaga catcaacatg
    11761 atcggcaact gcaaaacggc gcaagaagcg gaagtactgg cgcggcaggg gcaacagtta
    11821 atggcggaga ttcatgcttt gcccattccg gttatcgcgg ctattcatgg cgcttgcctg
    11881 ggcggcgggc tggagttggc gctggcgtgc cacggtcgtg tttgtactga cgatcctaaa
    11941 acggtgctcg gtttgcctga agtacaactt ggattgttac ccggttcagg cggtacccag
    12001 cgtttaccgc gcctgatagg tgtcagcaca gcattagaga tgatcctcac cggaaaacaa
    12061 cttcgggcga aacaggcggt aaagctgggg ttggtggatg acgtcgttcc gcactccata
    12121 ttgctggaag ccgctgttga gctggcaaag caggatcgcc catcttcacg ccctctacct
    12181 gtacgcgagc gtattctggc ggggccgtta ggtcgtgcgc tgctgttcaa aatggtcggt
    12241 aagaaaacag aacacaaaac gcaaggtaac tatccggcga cagaacgcat cctggaggtt
    12301 gtcgaaacgg gattagcgca gggcaccagc agcggctatg acgctgaagc acgggcgttt
    12361 ggcgaactgg cgatgacgcc gcaatcgcag gcgctgcgca atatcttttt tgccagtacg
    12421 gacgtgaaga aagatcccgg cagtgatgcg ccgcctgcgc cattaaacag cgtggggatt
    12481 ttaggtggtg gcttgatggg cggcggtatt gcttatgtca ctgcttgtaa agcggggctt
    12541 ccggtcagaa ttaaagatat caacccgcgg ggcataaatc atgcgctgaa gtacagttgg
    12601 gatcagcttg agggcaaagt tcgccgtcgt catctcaaag ccagcgaacg tgacaaacag
    12661 ctggcattaa tctccggaac gacggactat tgcggctttg cccatcgcga tctgattatt
    12721 gaagcggtgt ttgaaaatct cgaattaaaa caacagatgg tggcggaagt ggagcaaaat
    12781 tgcgctactc ataccatctt tgcttcgaac acgtcatctt taccgattgg cgatatcgcc
    12841 gctcacgctg cgcgacctca gcaagttatt ggcctgcatt tcttcagtcc ggtggaaaaa
    12901 atgccgctgg tggagattat tcctcatgcg agtacatcgg cgcaaaccat cgccaccacg
    12961 gtaaaactgg cgaaaaaaca gggcaaaacg ccaattgtcg tgcgtgacaa agccgggttt
    13021 tacgtcaatc gcattttagc gccttacatt aatgaagcta tccgcatgtt gaccgaaggt
    13081 gaacgggtag agcacattga tgccgcgctg gtgaaatttg gttttccggt aggcccaatc
    13141 caacttttgg atgaggtagg aatcgacacc gggactaaaa ttatgcctgt actggaagcc
    13201 gcttatggag aacgttttag cgcgcctgca aatgttgttt cttcaatttt gaacgacgat
    13261 cgcaaaggca gaaaaaatgg ccggggtttc tatctttatg gtcagaaagg gtgtaaaagc
    13321 aaaaaacagg tcgatcccgc catttacccg ctcattggcg cacaagggca ggggcgactc
    13381 tccgcaccgc aggttgctga acggtgtgtg atgttgatgc tgaatgaagc agtacgttgt
    13441 ctggacgagc aggttatccg tagcgtgcgt gacggggata ttggcgcggt atttggcatt
    13501 ggttttccgc catttctcgg tggaccgttc cgctatatcg attctctcgg cgcaggcgaa
    13561 gtggttgcaa taatgcaacg actggccacg cagtatggtt cccgttttac cccttgcgat
    13621 cgtttagttg agatgagcga gcgtggggaa agtttttgga aaacaactgc aactgacctg
    13681 caataagaag gtcaaagtta tatgaatccg cgctgaatgg cggagttttg ctcaaaatgt
    13741 aaacgcatat tgactatact tacgccattg aggtaaaaaa cagcgtttca ttcggtgaat
    13801 ggataaggca caatgccggc caccgttttc tttctctggt ttcagatgaa agaaaacggg
    13861 cgaatctggt taacaaaagc ggtgcaatat gcaagttttt atcatgcgtc acggcgacgc
    13921 agccctcgat gccgccagtg attcggttcg tcctctgacc actaacggtt gtgacgaatc
    13981 tcgcctgatg gcgaactggc tgaaaggtca aaaagtggaa atcgaacgcg ttctggtgag
    14041 cccgttcctg cgagccgagc aaacactgga agaagtaggg gattgtctga acctgccttc
    14101 cagtgcagaa gttctgccgg agttaacgcc ctgcggcgat gttggtctgg tcggtgccta
    14161 tttgcaggcg ttgaccaatg aaggtgttgc ttcggtactg gtgatctccc acttaccgtt
    14221 agtcgggtat ctggttgccg agttatgtcc gggcgaaacg ccgcctatgt ttaccacttc
    14281 agcgattgcc agcgtaacgc ttgatgaaag cggaaaaggc caattcaact ggcaaatgag
    14341 cccttgtaat ttgaagatgg caaaagcaat ttaagcaatc aatgcccggt tacgtgcgcg
    14401 tacaccgggc agtttctccg tcatacaatc attctttttc ttgtctaaat gttgttaata
    14461 tttaacaact ctcgatagtt attatatttt cccattattg aatcccgtaa taaaat
//
LOCUS       DAZFJL010000119        14178 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFJL010000119 DAZFJL010000000
VERSION     DAZFJL010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.120"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(95..1744)
                     /gene="pgi"
                     /locus_tag="ACHJ8C_003578"
     CDS             complement(95..1744)
                     /gene="pgi"
                     /locus_tag="ACHJ8C_003578"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO5553367.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGKVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      1981..2177
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2269..3618
                     /gene="lysC"
                     /locus_tag="ACHJ8C_003579"
     CDS             2269..3618
                     /gene="lysC"
                     /locus_tag="ACHJ8C_003579"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO5553368.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3871..4143
                     /locus_tag="ACHJ8C_003580"
     CDS             3871..4143
                     /locus_tag="ACHJ8C_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO5553369.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4144..5016)
                     /gene="rluF"
                     /locus_tag="ACHJ8C_003581"
     CDS             complement(4144..5016)
                     /gene="rluF"
                     /locus_tag="ACHJ8C_003581"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO5553370.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKSGDVVKVNGQLIEPRESEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5364..6311
                     /locus_tag="ACHJ8C_003582"
     CDS             5364..6311
                     /locus_tag="ACHJ8C_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO5553371.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQVKQKFGLKDVVVVSGNDEDEDTQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            6381..7187
                     /locus_tag="ACHJ8C_003583"
     CDS             6381..7187
                     /locus_tag="ACHJ8C_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5553372.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYESHKGYQFWPTDISSAKEINHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMAKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7197..7604
                     /locus_tag="ACHJ8C_003584"
     CDS             7197..7604
                     /locus_tag="ACHJ8C_003584"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO5553373.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLDKLVSIHNHDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNIDELCEHLTQIAKQTCVVWRQVATAEEDF"
     gene            7604..8098
                     /locus_tag="ACHJ8C_003585"
     CDS             7604..8098
                     /locus_tag="ACHJ8C_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO5553374.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            8164..8961
                     /locus_tag="ACHJ8C_003586"
     CDS             8164..8961
                     /locus_tag="ACHJ8C_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFO5553375.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIMLGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPSPQAISSTALDQLDD"
     gene            8973..9797
                     /locus_tag="ACHJ8C_003587"
     CDS             8973..9797
                     /locus_tag="ACHJ8C_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO5553376.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNLLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTPGADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9849..11078
                     /gene="sorE"
                     /locus_tag="ACHJ8C_003588"
     CDS             9849..11078
                     /gene="sorE"
                     /locus_tag="ACHJ8C_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO5553377.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAIGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            11460..12149
                     /gene="pepE"
                     /locus_tag="ACHJ8C_003589"
     CDS             11460..12149
                     /gene="pepE"
                     /locus_tag="ACHJ8C_003589"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO5553378.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKAAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12239..13870)
                     /locus_tag="ACHJ8C_003590"
     CDS             complement(12239..13870)
                     /locus_tag="ACHJ8C_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO5553379.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLPLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
BASE COUNT         3433 a         3574 c         3718 g         3453 t
ORIGIN      
        1 acctgtaggc ctgataagac gcgaccgcgt cgcatcaggc atcggttgcc ggatgcggcg
       61 tgaacgcctt atccggccta catatcgaca atgattaacc gcgccacgct ttatagcggt
      121 taatcagacc attggtcgag ctatcgtggc tgctgatttc tttatcatct ttcagctctg
      181 gcagaatacg gttcgccagt tgtttaccca gctccacgcc ccactggtcg aaggtgaaga
      241 tgttcaggat cacgccctga gtaaagattt tgtgctcata cagcgcaatc aacgccccca
      301 ggctgaacgg agtgatttca cgcagcagga tggagttagt cgggcggtta ccttcgaaca
      361 ctttgaacgg caccacgtag tcaagcgttg ccggatcttt accctgatcg cgatattcct
      421 gctcaaccac ttcgcgggat ttaccaaacg ccagcgcttc ggtctgagca aagaagttag
      481 acagcagttt ctggtgatga tcagagagcg ggttatgggt gatagccgga gcgatgaaat
      541 cgcacggtac cattttggtt ccctggtgga tcagctgata gaacgcgtgc tgaccgttcg
      601 tgccaggttc accccagata atcgggccag tctggtaatc cacaacctta ccgttacggt
      661 caacatactt accgttagac tccatattgc cctgctggaa gtacgccgcg aaacggtgca
      721 tatactggtc atacggcaga atcgcttcag tttccgcgcc aaagaaattg ttgtaccaga
      781 tgccaatcag cgccagcagt acaggcaggt ttttctcggc aggcgtggtg gagaaatgct
      841 tgtccatcgc gtgtgcgcca gaaagcagtt caacgaagtt atcaaagccg atggagagaa
      901 caatcgacag gccaatcgct gaccacaaag agtaacgacc gccgacccag tcccagaatt
      961 cgaacatgtt ggcagtatca ataccaaact cgccaacggc tttggcgttg gtggaaagcg
     1021 ccgcaaagtg tttcgcaacg tgtttctcat cacctgccgc tttcaggaac cagtcacgcg
     1081 cgctatgggc gttggtcatg gtttcctgag tggtgaaggt tttagatgct accaggaaca
     1141 gcgtggtttc cgggtttact tttttcagca cttccgcgat gtgagtccca tcgacgttag
     1201 aaacaaagtg catgttcagg tggtttttgt atggacgcag tgcttcagtc accatgtacg
     1261 ggccgaggtc agaaccgccg atcccgatgt tcactacgtc agtgattgcc ttgccggtat
     1321 agcctttcca ctcaccggaa ataatcgctt ccgagaaggt tttcatcttc tccagcactg
     1381 cgttgacttc cggcataaca tctttgccat caaccagaat cggggtattg ctacggttac
     1441 gcagcgctac gtgcagtacg gcgcggtttt cagtgcggtt gatcttctcg ccagagaaca
     1501 tcgacttaat cgcgcccgcc agatcgcact ctttcgccag atcttgtaat ttcgccagcg
     1561 tctcttcagt gatgcggttt ttggagtaat ccaccagcat ctgatcgtcg aaggttgcgg
     1621 agaacttaga aaaacgatcg ccgtctttag caaaaagatc ggcgatcgta acgtctttca
     1681 tttcatcgaa gtgtttctgt agtgcctgcc aggcagcggt ctgcgttgga ttgatgtttt
     1741 tcattagcaa tactcttctg attttgagaa ttgtgacttt ggaagattgt agcgccagtc
     1801 acagaaaaat gtgatggttt tagtgccgtt agcgtaatgt tgagtgtaaa cccttagcgc
     1861 agcgaagcat ttattagctg aactactgac cgccaggagt ggatgaaaaa tccgcatgac
     1921 cccatcgttg acaaccgccc cgctcaccct ttatttataa atgtactacc tgcgctagcg
     1981 caggccagaa gaggcgcgtt gcccaagtaa cggtgttgga ggagccagtc ctgtgataac
     2041 acctgagggg gtgcatcgcc gaggtgattg aacggctggc cacgttcatc atcggctaca
     2101 ggggctgaat cccctgggtt gtcaccagaa gcgttcgcag tcgggcgttt cgcaagtggt
     2161 ggagcacttc tgggtgaaaa tagtagcgaa gtatcgctct gcgcccaccc gtcttccgct
     2221 cttcccttgt gccaaggctg aaaatggatc ccctgacacg aggtagttat gtctgaaatt
     2281 gttgtctcca aatttggcgg taccagcgta gctgattttg acgccatgaa ccgcagcgct
     2341 gatattgtgc tttctgatgc caacgtgcgt ttagttgtcc tctcggcttc tgctggtatc
     2401 actaatctgc tggtcgcttt agctgaagga ctggaacctg gcgagcgatt cgaaaaactc
     2461 gacgcaatcc gcaatatcca gtttgccatt ctggaacgtc tgcgttaccc gaacgttatc
     2521 cgtgaagaga ttgaacgtct gctggagaac attaccgttc tggcagaagc ggcggcgctg
     2581 gcaacgtctc cggcgctgac agatgaactg gtcagccacg gcgagctgat gtcgaccctg
     2641 ctgtttgtcg agatcctgcg cgaacgcgat gttcaggcac agtggtttga tgtacgtaaa
     2701 gtgatgcgta ccaacgaccg atttggtcgt gcagagccag atgtagccgc gctggcggaa
     2761 ctggccgcgc tgcagctgct cccacgcctc aatgaaggct tagtgatcac ccagggattt
     2821 atcggcagcg aaaataaagg tcgtacaacg acgcttggcc gtggaggcag cgattatacg
     2881 gcagccttgc tggcggaggc tttacacgca tctcgtgttg atatctggac cgacgtcccg
     2941 ggcatctaca ccaccgatcc acgcgtggtt tccgcagcaa aacgcattga tgaaatcgcg
     3001 tttgccgaag cggcagagat ggcaactttt ggtgcaaaag tactgcatcc ggcaacgttg
     3061 ctacccgcag tacgcagcga tatcccagtc tttgtcggct ccagcaaaga cccacgcgca
     3121 ggtggtacgc tggtgtgcaa taaaactgaa aatccgccgc tgttccgcgc gctggcgctt
     3181 cgtcgcaatc agactctgct cactttgcac agcctgaata tgctgcattc tcgcggtttc
     3241 ctcgcggaag ttttcggcat cctcgcgcgg cataatattt cggtagactt aatcaccacg
     3301 tcagaagtga gtgtggcatt aacccttgat accaccggtt caacctccac tggcgatacg
     3361 ttgctgacgc aatccctgct gatggagctt tccgcactgt gccgggtgga agtggaagag
     3421 ggtctggcgc tggtcgcgtt gattggcaat gacctgtcaa aagcctgcgg cgttggcaaa
     3481 gaggtattcg gcgtactgga accgttcaac attcgcatga tttgttacgg cgcatccagc
     3541 cataacctgt gcttcctggt gcccggcgaa gatgccgagc aggtagtgca gaagctgcat
     3601 tttaatttat ttgagtaaac acagtatggc ccggaagaca ttatttcggg ccgtattgtt
     3661 tttcttgtca ttatgcccat aaataaacga gcctgtactc tgttaaccag cgtctttatc
     3721 ggagaataat tgcctttaat tttttatccg catctctaac taattatcga aagagataaa
     3781 tagttaagag aaggcaaaat gaatattatc agttctgctc gcaaacgaat tcccgtacac
     3841 tgaactctct ggctcactga gggcaccatc atggcactcc cccgtattac ccaaaaagag
     3901 atgactgaac gcgagcagcg tgaacttaaa acactgctgg atcgggcgcg gattgcgcat
     3961 ggtcgcgtac tgaccaactc cgaaaccaac agcattaaga aagagtacat cgataaatta
     4021 atggtcgagc gtgaggctga agcgaaaaaa gcccgccagt taaagaaaaa gcaggcttat
     4081 aaaccggatc ctgaggcgtc atttagctgg tcagcaaata cgtcaacgcg cggcaggcgc
     4141 tgattaacgc cccttctttt tacgccccgg cgaggtaaaa cgcttaccgt tggacgccgg
     4201 gcgaccgcct ttttccgccg ttttttccat cttaacgact ggacgtttga tgcctaccgt
     4261 tttcggtttg gccttcgctt taggttttgc ctcggaagag gaattttcaa tgagcttaaa
     4321 gagatcgatt aactcatcgt cggttaaatc gcgccactca cccagcggaa tgccgcttaa
     4381 gctgacgttc ataatgcgcg tacgttccag ctttttcact tcgtaaccaa agtgctcgca
     4441 catgcgacga atctgacggt tcagcccctg caccagggta atgcggaaga caaacggtgc
     4501 ttcttttttg actttgcact ttttagttac cgtcccgagg atcggtactc ccgcccccat
     4561 accacgaata aattcatcgg taatcggttt atcaaccgtc accagatact ctttctcatg
     4621 atcgttaccc gcacggagga tcttattcac cagatcgccg tgattggtga ggaaaatcag
     4681 cccctgggaa tctttatcca ggcggccaat cgggaacacg cgtttgctgt ggttaacgaa
     4741 atcgacaatg ttatcgcgct ctccatcttc ggtggtgctg acaataccga cgggcttgtt
     4801 cagggcgata agtaccaaat cttcggattc ccgaggctca atcaactgac cgtttacttt
     4861 cacaacgtcg ccggatttca cctgatcgcc aatggtggct cgcttgccat taaggaacac
     4921 attgccttgc tcgatatagc gatccgcttc gcggcgtgag caaattccgc tttcgctgat
     4981 gtatttattt aaacggactg atgagtcggg cagcataaat tctcctgtaa aacgcggaat
     5041 ataccgtacc tcttagcggg gaaaaacagg gttatggggc aacgtcgaaa ttaaatgtga
     5101 tctgccacgc cttttatcac aaaagtgtct ccggaagtac gatgcaaatt tgcctggcaa
     5161 aataaggata atcaacaaat tacgaatcgc tgcacacctg tgctgatgac tactacgcag
     5221 agaaacaaat gtgcaagaga aagcgctatt tccccccatg caaagagtga aaaatcgctt
     5281 tgaaaggcca tcatgatggg gttatatcag atcaagcggt gagaaagcgt atttgcacaa
     5341 atgtgcagaa ccaggaggcg gcaatggaaa acagtgacga tatccgtttg attgtgaaga
     5401 ttgcccaact ctattacgaa caggatatga cgcaggcgca aattgcgcgc gaactgggta
     5461 tttaccgcac caccatcagc cgcttgctta aacgaggccg cgatcaggga attgtcacca
     5521 tcgccatcaa ctatgactac aacgaaaatc tctggctgga gcagcaagtg aagcaaaagt
     5581 ttggcctgaa agacgttgtg gtggtgtcag gaaatgatga ggatgaagat actcaactgg
     5641 cgatgatggg gttacacggc gcgcaactgc tggatcgctt gctggaacct ggcgatattg
     5701 ttggtttttc ctggggtcgc gcggtgagcg cactggttga aaacttgccg caggcggggc
     5761 aatcgcggca gttaatttgc gtgccgatta ttggcggccc gtccggtaaa ctcgaaagcc
     5821 gctatcacgt aaacacatta acctacagcg cggcagcgaa gctgaaaggg gaatcgcatc
     5881 tcgcggattt tccggctctg ctggataacc cattaattcg taatgggatc atgcagtctc
     5941 agcactttaa aaccatctct gcctactggg ataatctgga tgtcgccctg gtgggaattg
     6001 gctcaccggc cattcgcgac ggcgctaact ggcatgcgtt ttatggtggt gaagagagtg
     6061 acgacctgaa tgcccgccag gttgctggcg atatttgctc gcgctttttt gatattcacg
     6121 gcgcaatggt tgaaacgaat atgagcgaaa aaacactctc tatcgaaatg aataaattaa
     6181 agcaggcacg gtattccatt ggcattgcca tgagcgaaga aaaatacagc ggaattgttg
     6241 gtgcactgcg tggaaaatat attaattgtc tggtaacgaa tagcagcaca gctgaactat
     6301 tactgaaata aaaatcttcc cgatgtttac cgtaataaaa attacggcgg gtccctatta
     6361 tctaaaatca ggagtctgtt atgcaaacgt ggttaaattt gcaggataaa atcattattg
     6421 tcactggtgg cgcatccggc attggtctgg cgattgtcga agaattatta gcacaaggcg
     6481 cgaatgtgca gatggtcgat attcacggtg gcgatggtca gtatgaaagc cataaaggtt
     6541 atcagttctg gccgaccgat atttccagcg ccaaagagat aaatcatacg gtagcagaaa
     6601 ttatccagcg ttttggtcgc atcgacggtc tggttaacaa cgccggggta aatttcccgc
     6661 gtctgcttgt cgatgagaaa gcgcctgccg ggcagtatga actcaacgaa gctgcattcg
     6721 aaaaaatggt caatatcaac cagaaaggcg tttttctgat gtcgcaggcg gtggcgcgac
     6781 agatggccaa acaacatgat ggcgtgattg tgaatgtttc ctcagaaagt gggctggaag
     6841 gctcagaagg ccaaagctgt tacgccgcga ccaaagccgc gctcaatagc ttcacgcgct
     6901 cctggtcgaa agagctgggt aagcacggta tccgtgtggt cggtatcgcg ccggggattc
     6961 tggaaaaaac aggactgcgc acgccggaat atgaagaagc gctggcgtgg acgcgcaata
     7021 tcaccgtcga gcagctgcgt gaaggctata ccaaaaacgc cattcctatt gggcgcgccg
     7081 gaagattagc agaagtggct gattttgttt gttatctgct gtctgaacgc gccagctata
     7141 tcaccggagt aaccactaac attgcgggcg gcaaaacgcg cggctaagga ggctttatgg
     7201 tcaatgccat tttttgcgcc cacggcaaat tagcctgcgc catgctggaa tcggttcaga
     7261 tggtgtacgg cgatgccagg gtcgaggcag ttgaatttgt gcccggcgag aacgccggag
     7321 acattgtggc aaagctggat aagttagtga gcattcacaa tcatgacgag tggctaatcg
     7381 ccgtggattt acagtgcggc agcccgtgga atgcggcagc gatgcttgcg atgcgcaatc
     7441 ctcgtctgcg ggtaatcagc ggcctttctc tgccgctggc gctcgaactg gttgataacc
     7501 aggacagcat gaatattgat gaactgtgtg aacacctgac gcaaatcgca aagcaaacct
     7561 gcgtcgtctg gagacaggtg gcaacagcgg aggaggattt ctgatgaaca ttacgctcgc
     7621 ccgcattgat gaccgcctga tccacggcca ggtcactacc gtctggtcta aagtggctaa
     7681 cgcccaacgc attattatct gcaatgacga agtttataac gatgaagttc gccggacatt
     7741 attgcgtcag gctgcgccac cgggaatgaa ggttaacgtg gtcaatattg aaaaagccgt
     7801 cgcggtttat cacaacccgc aatatcagga cgaaaccgtt ttttatcttt tcactcgccc
     7861 acaggatgcc ttagcaatgg ttcgccaggg ggtaaaaata ggtaccctga atattggcgg
     7921 tatggcctgg cgtcctggta agaaacagtt aaccaaagca gtttcattag atgacgatga
     7981 tattaacgca tttcatgagt taaataatct tggcgtcata ctggatttac gcgtcgtagc
     8041 gtcagatccc tcaatcaata ttatcgacaa aataaacgaa cagttaatcg caaattaaat
     8101 aatagcgcct gtgtacatat gctttaacag gcagggatga cgacacttaa aaggtgacaa
     8161 atcatggaaa taagtaccct acaaatcatt gctatatttc ttttttcctg tattgccgga
     8221 atgggcagcg tgctggatga atttcagact catcgtccgt taattgcctg tacggtgatt
     8281 ggtttaattc tcggtgattt aaaaaccgga attatgctcg gtggtacgct ggaattgata
     8341 gctctcggct ggatgaacgt cggcgcggcg caatctccgg attctgcact cgccagcata
     8401 atctccgcca ttctggttat cgttggtcag caaagcatcg ccaccggaat cgccatcgcg
     8461 ttgcctgtgg ctgcggcagg ccaggtgctg acggtgtttg cccgtaccat caccgtagtg
     8521 ttccagcacg cggcggataa agcagcggaa gaagcacggt ttcgcactct cgatattctg
     8581 catgtctccg cgcttggcgt gcaggcactg cgcgttgcta ttccggcact gattgtctcg
     8641 ctgttcgtca gcgctgatat ggtgagcaat atgctgagcg ccattcccga atttgtgacc
     8701 cgtggactgc agattgctgg cggttttatc gtggtggtcg gttacgccat ggtgcttcgc
     8761 atgatgggcg tgaaatattt gatgcctttc tttttcctcg gtttcctcgc aggtggctac
     8821 ctcgatctca gtctgctggc gttcggtggc gtcggcgtga tcatggccct gctctacatc
     8881 cagttaaatc cacagtggcg taaagctgaa ccctcccctc aggctatctc ctccaccgcc
     8941 cttgaccaac ttgacgatta atggagctca tcatggaaca gagaaaaatt acacgcagcg
     9001 atctggtgag catgtttctg cgctccaacc tgcaacaggc gtcctttaac tttgaacgta
     9061 ttcacgggct gggcttttgc tacgacatga tccccgccat caagcgactc tacccattaa
     9121 aagaggatca ggttgcggcg ctcaggcgac atctggtgtt ctttaatacc acgcctgccg
     9181 tgtgtggtcc ggtcatcggc gtcactgccg ctatggaaga agcgcgagcc aacggtgcag
     9241 aaatagatga cggcaccatt aacggcatca aagtcggcct gatgggaccg ctggcaggag
     9301 ttggcgatcc actggtctgg ggaacgctgc gcccgattac cgccgcgctt ggcgcatctc
     9361 tggcactttc tggcaacatt ctcggcccgc tgctgttctt ctttattttc aacgcggtgc
     9421 gtctggcaat gaaatggtat gggctgcaat tgggctttcg taaaggggtg aatatcgtca
     9481 gcgatatggg cggtaatttg ctgcaaaaac tgaccgaagg cgcatcgatt ctcgggctgt
     9541 ttgtaatggg cgtgctggtg accaaatgga catcgatcaa cgtgccgtta gtggtttcac
     9601 aaacgcctgg cgccgatggt tccaccgtca ctatgaccgt acagaacatt ctcgaccagc
     9661 tttgccctgg attgctggcg ctcggtttga cgctgctgat ggtacgtctg ctcaacaaaa
     9721 aaattaaccc ggtatggctg attttcgccc tgtttggctt agggattatc ggcaatgcgc
     9781 tgggcttcct gtcctgattc ttcgccccgg cacgactgcc ggggccatcg ctcaacatga
     9841 ggtggtttat gaaaacaact gctctgcgtc tttatggtaa acgtgattta cgcctggaaa
     9901 cctttgacct tcctgaaatg caggaggatg aaatcctcgc gacggtggtc actgacagcc
     9961 tgtgcctctc ttcctggaaa gaggccaatc tgggggaaaa ccataaaaaa gtacccgacg
    10021 atgtggcgac caatcccatc atcatcggcc acgaattttg cggcgatatt ctggccgtgg
    10081 gtaaaaagtg gcagcacaaa ttccagccgg gtcagcgtta tgtgattcag gccaacctgc
    10141 aactccccga ccgcccggat tgccccggct actccttccc gtgggtaggc ggcgaagcga
    10201 cgcatgtggt tattcccaac gaggtcatgg aacaagattg cctgctggca tacgacggcg
    10261 aaacctattt tgaaggctcg ctggttgaac cgctttcctg cgtgattggc gcgttcaacg
    10321 ccaactatca tcttcaggaa ggtagttata accacacgat ggggattcgc ccgcaagggc
    10381 gcacgctgat cctcggcggc accggcccga tggggctgct ggcgattgat tatgcgttgc
    10441 atggaccggt taacccgtcg ctgctcgtca ttactgatac cgacaacgac aaattgagtt
    10501 atgcacgcaa gcactatcca tcagaaccgc aaacactgat tcattatctc aatgccgccg
    10561 atgcagcatt tgatacgtta atggcactga gtggcggtca cggcttcgat gatattttcg
    10621 tctttgtgcc taatgaagga ctggtgactc tcgcctcttc cttgctggcg acagatggtt
    10681 gtctgaattt cttcgccgga ccgcaggata agcacttcag cgcgccaatt aatttctacg
    10741 atgtgcatta tgcatttacc cactacgtgg gcacatcagg cggcaatacc gacgacatgc
    10801 gcgcagcggt caaattgatt gaagagaaaa aagtgcaggc cgcaaaagtg gtaacacata
    10861 ttcttgggct gaatgccgcg ggcgaaacca cgcttgaatt gcctgccatc ggcggcggca
    10921 aaaagctggt gtataccggg aaatacctgc cgctgacgtc actcacgcag attcaggatc
    10981 aagcactggc ggcaattctg gcgcgtcatc aggggatctg gtccggtgag gcggagcaat
    11041 atctgctcac tcatgcagag gcaatttccc atgattaatc gcgctactta actctgcatg
    11101 tcgctggctg gtcgccgcgg caacttcgcg acctgatgtg cggctgattg ctgaagcggc
    11161 acaatgtgca cgagaaggat aaaaaaacat cagcgcctta aggggcgctc aaattggtgg
    11221 caaagtgcac attgtttatg ccggatacgg aatgaagcct tattcggcct acataatctt
    11281 gaaaattcaa tgtattgcag ttatctggta ggccaagtaa gcgtagcgca tcaggcaatc
    11341 ctgtgtttgt catcagtctc agcgcctgtg caatatgtga catcgtgagc catgtcagta
    11401 agtggttaaa ttcgcagtgg tagcattcct tccgtgtttt cttactaacc ggagaagtaa
    11461 tggaactgct tttattgagt aactcgacgc tgccgggtaa agcctggctg gaacacgcgc
    11521 tgccgctgat tgctgaacag ttgcagggtc gccgttctgc ggtatttatt cctttcgctg
    11581 gcgtaacgca gacctgggat gattacacag cgaaagcggc tgcggttctc gctccgctgg
    11641 gtgtttctgt caccggtatt catagcgttg tcgatcccgt tgccgcgatt gaaaatgcgg
    11701 aaattgtgat tgtcggcggc ggtaatactt tccagttgct gaaacagtgc cgcgagcgcg
    11761 ggctgctggc acccattact gacgtggtga aacgtggcgc gctgtatatt ggctggagcg
    11821 caggcgctaa ccttgcttgc ccaaccattc gtaccaccaa cgatatgccg attgtcgatc
    11881 cgcaaggttt cgatgcgcta aatctgttcc cgctgcaaat caacccgcac ttcaccaacg
    11941 cgctgccgga aggccataaa ggtgaaaccc gtgagcagcg tattcgcgaa ctgctggtcg
    12001 tcgcgccaga actgacgatt attggtctgc cggaaggtaa ctggatcaca gtaagtaaag
    12061 gtcacgctac gctgggtggc ccgaacacca cttatgtgtt taaggctggt gaagaagcgg
    12121 tgccgctgga agcgggtcac cgtttttaag tgaattgccg gatgcatgat ccggcatctt
    12181 gttcatagtg cctgatgcga cgctttacgc gtcttatcag gccttcaggg tcacggtatt
    12241 aatactcgtc ccgtccttca tcttcatccg gttgttccag cacactgtaa gccaccgaac
    12301 aaaacagcga gttcagacgc tgcatatctc ccagtaagcc taaatgtagc gaactggttt
    12361 caatactctg cacattttgc tgatgcaggc gatcgacgtg ggcgtgggaa tagcggcgat
    12421 taagaatgcg aaaacgatgt ttgctgcggc gcaaacgacg ggcgctggta acatcgccag
    12481 agaagaaaac cgacattgcc agttttaaat tactgagcaa ttgctcatag agcgcatcca
    12541 gttccttcaa gccatcaagc gaaaatgctc gccttgcagc cagcgatttg tcggcaattt
    12601 cgctgcccat gcgctcgacg atatcggagg cctgttcaag gttgagcgac atttcgatga
    12661 tctccgccca gcggcgcgac tcttcttctg ccagttcctc ttttggcatc cgcgccagat
    12721 acagcttaat ggcggtatag agaacgttga tgtcatcagc cagcttacgc agctctttct
    12781 cctgccgtgg ctcgccgtgc atcactttat tcagcccttc catcatctgt tccatggcgt
    12841 cgccaatgcg cagggtttcg cgggcagcgt tcgccagagc aagcgtgggc gtatccagcg
    12901 cgctgacatc caaatgttta ggccgtagct gggtatccag ttccggttca tcgcgaataa
    12961 tcgttttgca aaaccgtgcc atcgggtcaa cgaatggcaa catgaccagg cagcgtacaa
    13021 ggttgtagaa gacgtggaaa tagatcacca attccgcttt tggcaatggc aacttcccca
    13081 ttgtctctgc cagcaaatga acaaacggca ggataatcag gctacccacc agcttaaaca
    13141 gcaggctacc cagcgcgaca cggcgggctg cggcattggc ggcactgttg ttgagcatcg
    13201 ccagcaggcc ggaaccgagg tttgcaccaa tcaccagaca aagcgccact gggaaagaga
    13261 taatgcctgc ggcggtcaga gtcgcggtca gcagtacagc agcaaggctg gagtagctga
    13321 taatggcgaa catcgcacca atcagcgcat ccagcaaaat gtcgccagtc agtgaggcaa
    13381 agatcacctg aacgccgttt gcctgagtga tcggcgttac ggcctgcaca atcaactcca
    13441 gcgccagtaa aatcagccca agaccaatac cgacgcggcc cagttgcccg gcgcgtgact
    13501 gtttgcgtcc gaggaaaaaa atcacgccga taaaaataag taacggtgac agccaggata
    13561 aatcgaaggt gagaatacgc gccatcagcg ccgtcccgac atctgcaccc agcacaatca
    13621 ccagcgcagg cgcgagagca accagatcct gagcgacgaa cgaggtcacc agcatggtgg
    13681 tggcattact gctctgcacc agtgcggtaa cgccgatccc cgcgcaaaag gcgagcggct
    13741 ttttttcgac gctccggcta aggacagtac gcagacgcgc gccgaagacg cgcattacgc
    13801 ctgttcgaac aatatgagtc ccccagacca gcagggcgac ggcagaaagc aggtgaagca
    13861 aagttagcac ggaatcaggt tctccttttt gttatgcatt ttcctgttaa gatttttcta
    13921 atcacgttgt ttttatccag gcatttctct tgtatgtact ttcagtataa gagatttata
    13981 caagaaagca tttaaaaagg catttatata aaaatccacc tgtatctgtt gctatttctg
    14041 tcacactttc ccctgtcgtt tacaccagat aatcgttgtc acttttgtga cctctgagaa
    14101 gataaacaac gaaatattta agaccagatt ctgtgaatga aaataattaa gtaacaacgg
    14161 tattacggag attgagca
//
LOCUS       DAZFJL010000120        13922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFJL010000120 DAZFJL010000000
VERSION     DAZFJL010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.122"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..104)
                     /locus_tag="ACHJ8C_003591"
     CDS             complement(<1..104)
                     /locus_tag="ACHJ8C_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO5553380.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDHAIAYK"
     gene            complement(101..577)
                     /locus_tag="ACHJ8C_003592"
     CDS             complement(101..577)
                     /locus_tag="ACHJ8C_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO5553381.1"
                     /translation="MQVLNSQRKAFLDMLAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPQLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKVGSLIAKFKEAGGTVREIEV"
     gene            complement(581..916)
                     /locus_tag="ACHJ8C_003593"
     CDS             complement(581..916)
                     /locus_tag="ACHJ8C_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFO5553382.1"
                     /translation="MPHNPNTWPDWLELFQSWWRGDTPLGAVIMSIVMAGLRIAYFGG
                     GGGWKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMRVI
                     GNKFGGSKE"
     gene            complement(993..2045)
                     /locus_tag="ACHJ8C_003594"
     CDS             complement(993..2045)
                     /locus_tag="ACHJ8C_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO5553383.1"
                     /translation="MVNTVKISSCELINADCLEFIQTLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNRCNKESLRAYFPATERILFAEHYQGPYQPKNDGYAAKERELKQHIMA
                     PLISYFRGARAELGITAKQIAEATGKKNMVSHWFGASQWQLPNEADYRKLQALFSRIA
                     AEKFQEQQLEQPHHQLVASYDSLNRKYSELLDEFKSLRRYFSVSVSVPYTDVWTHKPV
                     QFYPGKHPCEKPADMLRQIINASSRPGDLVADFFMGSGSTIKAAMALGRRALGVELES
                     ERFNQTAKEVSELVGK"
     gene            complement(2196..2393)
                     /locus_tag="ACHJ8C_003595"
     CDS             complement(2196..2393)
                     /locus_tag="ACHJ8C_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO5553384.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            complement(2618..3181)
                     /locus_tag="ACHJ8C_003596"
     CDS             complement(2618..3181)
                     /locus_tag="ACHJ8C_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO5553385.1"
                     /translation="MNNVSVENLPSLTHQHLPVITTELLAELYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDELRELKHRNSLRVPVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFSQKQPPAAQNSLNENDGCALLIHFDKHGQVDFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRL"
     gene            complement(3540..3659)
                     /locus_tag="ACHJ8C_003597"
     CDS             complement(3540..3659)
                     /locus_tag="ACHJ8C_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004977456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO5553386.1"
                     /translation="MTRHIVRGYIPHASAKSGVGIGTPDIETVHNRAGGFFMR"
     gene            complement(3664..4050)
                     /locus_tag="ACHJ8C_003598"
     CDS             complement(3664..4050)
                     /locus_tag="ACHJ8C_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO5553387.1"
                     /translation="MARDIQQVMERWGAWAANNHEDVSWASIAAGFKGLIPPKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVYGMTFMSLARKLGCSDTCVGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(4040..4417)
                     /locus_tag="ACHJ8C_003599"
     CDS             complement(4040..4417)
                     /locus_tag="ACHJ8C_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5553388.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGECYRRDVALIVR
                     QQRLKLNLSGRLAIRIAAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQVVPGGRLGVKIYEITGDNDGA"
     gene            complement(4418..5476)
                     /locus_tag="ACHJ8C_003600"
     CDS             complement(4418..5476)
                     /locus_tag="ACHJ8C_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5553389.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIVPAVRQPLAEDKSLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGAGAMVLCWHCDNQLRDQTSESLGQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAVCNQVADALPESVLRRSLGLRADKIRSVYRESDIVPGEQSATSILKQR
                     TKNLAPLSHVHQQQIPPQEKTVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEDRHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            complement(5478..5756)
                     /locus_tag="ACHJ8C_003601"
     CDS             complement(5478..5756)
                     /locus_tag="ACHJ8C_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553390.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKYDWYQHDPCTEEQAEW
                     LIHNYRRRGYEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(5924..6079)
                     /gene="hokD"
                     /locus_tag="ACHJ8C_003602"
     CDS             complement(5924..6079)
                     /gene="hokD"
                     /locus_tag="ACHJ8C_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO5553391.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            6711..7619
                     /locus_tag="ACHJ8C_003603"
     CDS             6711..7619
                     /locus_tag="ACHJ8C_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5553392.1"
                     /translation="MKNDIKTLDLNLLKTFDALMDEGSVTRAAQRLSLTQPAVSGMLI
                     RLRDYFCDPLFVRTSHGMVPTLRAKELAMPVKQILTDIAILLKPSKFDPMTAELTYTI
                     VATDYAVKAVVVPLMAALKHRAPNIKISVRPVDNKRIYQQLSQGEVDLALVTPQTTPG
                     ELHGRALYEEDYVCIARRNHPLSANSEMTLEQFCKQEHILVSSEGSFSGVTDEALAKL
                     SLTRRVGMSVNSFQVVPDILQVTDMIAVVPHRMVLTNNDLIILPLPLKVPGFTKSMAW
                     HERTHRDPSHQWIRALCVEVSQGTGS"
     gene            7755..8261
                     /locus_tag="ACHJ8C_003604"
     CDS             7755..8261
                     /locus_tag="ACHJ8C_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HFO5553393.1"
                     /translation="MNAIHWPEGFVPGFTDNFVSNEMIISGLNINDVWPLLSQPLLWP
                     EYYKNSADVRFYNNKGPELENGVRFYFSTFGFPVEAQVVEFIPPTEGKPARLAWHGWS
                     GEKDTVQRLDVHHAWLLEELSGNRLRILTQETQNGVPAKDLANTRPNPMLNGHQEWLD
                     GLILAAKK"
     gene            complement(8490..8915)
                     /locus_tag="ACHJ8C_003605"
     CDS             complement(8490..8915)
                     /locus_tag="ACHJ8C_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO5553394.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLESKTGATRYLIS
                     VLARELVASGDIYNSGYGLFPSEQARKDWQNARKKLSRENLKKPSMVDPDLIWSLPDG
                     EIRRYDRHLNIICRECRESEVMQRILAFYQGNVRYFRRY"
     gene            complement(8956..10026)
                     /locus_tag="ACHJ8C_003606"
     CDS             complement(8956..10026)
                     /locus_tag="ACHJ8C_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFO5553395.1"
                     /translation="MANAWLRLWHDMPNDPKWRTISRVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDKDTDKDTDKEDQN
                     TMVHGVKNATNQAGNVQTVNPGQPAGTTPEADSAYALKADSGAVQQVMTARPEQSHQL
                     QQPEADSAIQREADRVVPEKTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWK
                     ARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRATTFLGPDRNFENPWLLPVSGT
                     NNQRCVNHISEPDTEIPPGFRG"
     gene            complement(10098..10523)
                     /locus_tag="ACHJ8C_003607"
     CDS             complement(10098..10523)
                     /locus_tag="ACHJ8C_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO5553396.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAEITQAYFELGMTFPELYD
                     DRHPEALARNTQKIFRWLDKDTPDAVKKIQALLPAIEKAMPPLLVARMRSHSSAWFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNIVVVH"
     gene            complement(10520..10822)
                     /locus_tag="ACHJ8C_003608"
     CDS             complement(10520..10822)
                     /locus_tag="ACHJ8C_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5553397.1"
                     /translation="MTLKEFIKSLRVGDAKKFAARLGVSPSYLSQMASGRAAISPTRA
                     LMIESATEGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
     gene            10920..11291
                     /locus_tag="ACHJ8C_003609"
     CDS             10920..11291
                     /locus_tag="ACHJ8C_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553398.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPEISPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            11312..11503
                     /locus_tag="ACHJ8C_003610"
     CDS             11312..11503
                     /locus_tag="ACHJ8C_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HFO5553399.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFARMRKERSKQ"
     gene            11505..11783
                     /locus_tag="ACHJ8C_003611"
     CDS             11505..11783
                     /locus_tag="ACHJ8C_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5553400.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
     gene            12136..12435
                     /locus_tag="ACHJ8C_003612"
     CDS             12136..12435
                     /locus_tag="ACHJ8C_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553401.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRAMRFKGCVKDFAATFWARKLDCIHNQFPLE"
     gene            12507..12725
                     /gene="ydfC"
                     /locus_tag="ACHJ8C_003613"
     CDS             12507..12725
                     /gene="ydfC"
                     /locus_tag="ACHJ8C_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO5553402.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMRQKINAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            12748..13155
                     /locus_tag="ACHJ8C_003614"
     CDS             12748..13155
                     /locus_tag="ACHJ8C_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553403.1"
                     /translation="MAFKDLPKEIQTIAATTLGDSLVKIDPAYTKKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENDANLRGRTIAHPYAHTLGSVDIKDPTNL"
     gene            complement(13133..13366)
                     /locus_tag="ACHJ8C_003615"
     CDS             complement(13133..13366)
                     /locus_tag="ACHJ8C_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553404.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
BASE COUNT         3533 a         3578 c         3205 g         3606 t
ORIGIN      
        1 tctttgtagg cgatggcgtg atcacggtaa tgattaaccg cccatgacag gcagacgatg
       61 atgcagataa ccagagcgga gataatcgcg gttactctgc tcatacctca atctctctga
      121 ccgttccgcc tgcttctttg aattttgcaa tcaggctgcc aactttatgc tcgaactgac
      181 cataaccagc gcccggcagt gaagcccaga tattgctgca acggtcgata gcctgacgaa
      241 tatcaccgcg atcaatcatt ggtaaagcgc cacgctcttt aatctgctgc agtgcaacag
      301 cgtcctggct tttcggagag aagtctttca ggccaagctg cttgcggtag gcatcccacc
      361 aacgggaaag aagctggtaa cgtccagctg cggttgattt aagttgcggg tttagcgtga
      421 caagtttgcg aggatgatcg gagtaatccg tgaatagctc accacctaca ataacgtcgt
      481 agccgtgatt gcgggttggc tgtcgcccgt tatccgttcc ttctgaccac gccaacatat
      541 cgaggaaagc tttacgctgg gaatttagta cctgcataaa ttactcctta gagccaccaa
      601 acttgttacc gattactcgc attgcagccc cacgaatagc atcgacaccg atcagcccca
      661 cgccgccacc aatagcaaca gaaagtgatt taggccatcc gacatactca agagcggatg
      721 caaaggtcag cgtcagagcg ccacagagca aaatctcgag cgtttttcgc ttccagccac
      781 caccaccgcc aaaataggcg atgcgcaaac cagccataac gatcgacata atcactgcac
      841 ccagcggtgt gtctccacgc caccagctct gaaacaactc cagccagtcc ggccaggtat
      901 ttgggttatg aggcatttcg tcatctctca cctcgcgata tttgcgggtg ctgtgttgga
      961 aataaaaagg ccacgcaacg tggccaccag aattatttcc ccaccagttc acttacctct
     1021 ttcgccgtct gattaaaccg ctctgactca agttcaacac ctaacgcccg acgccccagc
     1081 gccattgctg cttttattgt ggaaccggat cccataaaga aatcagcaac cagatcacca
     1141 ggtcgactac tggcattgat tatttgccgg agcatatccg ccggtttctc gcacggatgt
     1201 ttacccgggt agaactgaac gggcttatgc gtccagacat cggtataagg cacggagact
     1261 gatacggaga aatagcgccg gagagattta aactcatcca gcaattcaga atatttgcga
     1321 ttcagtgaat cataagatgc caccagctgg tggtgtggtt gttccagttg ttgttcctga
     1381 aacttctctg ccgctatacg ggaaaacagc gcctgtaact tccgatagtc agcctcattc
     1441 ggcaactgcc actgactggc accaaaccag tgggaaacca tatttttctt acctgtggct
     1501 tcggcaattt gttttgccgt tatacccagt tcggcacgag cacccctgaa atacgatatc
     1561 agcggtgcca ttatgtgctg tttgagttcc ctttcttttg ccgcatagcc gtcatttttg
     1621 ggctggtatg gcccctgata atgttcggca aacagaatgc gttctgttgc cgggaaatac
     1681 gcccgcaggc tttccttgtt gcacctgttc cagcgtcctg acggcttcgc ccagataatg
     1741 tgattcagca cattaaagcg ttcacgcatc atgatttcgg tgtcagatgc caggcgatga
     1801 ccacagaaca ggtagagact tccggcaggt ttcagtaccc gccagaattg cgccagacac
     1861 tgatccagcc atttcaggta atcatcgtcg cccttccact ggttatccca gccctcgggc
     1921 ttcactttaa agtatggcgg gtctgtgact atcagatcga cagagttttc cggtaaggtc
     1981 tggataaatt ccaggcaatc agcgttgatt aactcacaac tggatatttt tacagtatta
     2041 accatagatc aataagcact tctctgatag gctcataccg cttttgcgca aagcagatgg
     2101 gcctgaggtt tgcttgtgac cccaacgcat gagcagatgg ctgataggtg ccgctaacac
     2161 ccaccagccg cccattacca caaattaaaa agccttcact gcggaaggcg tctgtaacaa
     2221 ccgaactgat aatctgccag acccgccata acaagctggg tcagtattaa ctggcagcgt
     2281 tcgcgtgaaa ggtaagtatt ctgcgcaatt tccccgacgg tcgccggttc ggtgacgctt
     2341 aattcattaa acaccactct ggcggtttcg gtcatatcct gctgttttag catgtctttt
     2401 tcccttttct ggttaacgtg acataccagt aactcttgtc gaaaaagcca gcaagctgaa
     2461 agaccggtat tcgcaaccac cagcgcgttt aacgtcctgt gccacttttc gggcataaaa
     2521 aaacccgctc atcggcgggt ttaagctatg tggcgtagta accactctta acaggatatt
     2581 caacttttta cgatcgtaaa gcgttcggag aaaattttta aagccgcatc aaccgttcca
     2641 ccagttgttc tttacgggca acgatccagc cgtgttgctc cagataaaat ttaaaccgtt
     2701 ccagagtgca taccatcgca tcggcgggta ctttttccgt gaagtcgacc tggccgtgtt
     2761 tatcgaagtg gatcagtaat gcgcatccat cattttcgtt gagggagttt tgtgctgctg
     2821 gtggttgttt ttggctgaaa taacagtctt cgagtttttc gaacacttcc cacgcctgat
     2881 cggtttcgag catttttgcg tggcgggctg ctccgcgttc ggtccagagg atgagggagc
     2941 gaacgttacg ggcaattttc acaggaactc ttaaagagtt tctgtgcttc agttcccgta
     3001 attcatcgcc ttcgatcagg aaaaagtgct ttccttcaat aaaccgctct ttgtttctat
     3061 tgaaattatt tgtcaaacgc tggcgctctg ttccgtacag ctcagccaga agttctgttg
     3121 ttataacagg aaggtgttga tgtgtaagtg atgggagatt ttcaacagat acgttgttca
     3181 tgaatatgtc ctactgaata ttttgattta ccccttttga gagggtgacc gggcgctcaa
     3241 aaccgtcagt agtcggcggg cgtattccca ttgcaggtat tgtattagcc gcacgcccgg
     3301 tcataaacca agaattctgg acataaaaaa accgcatgtc tgtcgggcgc ggtaaccgct
     3361 actgaaaggt gttttgagca ccgtcaagaa atgtaatctg ctaacatgaa actgtcaata
     3421 cattcatgtt ccaatcgctg ggtggtgaac tgtgcagggt tggaactacc gttcgaaaca
     3481 tccggcgcac ctttcggtgc ccccacacag tccaccataa atcgcgaatg tgactgtgct
     3541 tagcgcataa aaaaaccgcc agcgcggtta tgcaccgttt cgatatccgg ggttccaatc
     3601 ccgacgccag attttgctga cgcgtgagga atatagcccc ggacaatgtg tctggtcaag
     3661 cacctacata attcgttcta cgtatctgtc catctccagc cggatatcaa gcatcatcaa
     3721 catgccatcg ataacccctt ccgccttctg caggcgctta ccaacacagg tatccgaaca
     3781 tccaagtttc cgtgccaggc tcataaaagt cattccatat acgtaataat ccaccagcaa
     3841 atcgtgcaag tcctgatttt tcttgttcaa ccgggccata cagccacaaa ttatcattgc
     3901 atcatcatca gaacactgag gacgtgattt cactttcggc gggattaatc ctttaaaacc
     3961 agcagcgatt gacgcccagg acacatcttc gtgattgttt gcagcccatg ccccccaccg
     4021 ctccataacc tgctgaatat cacgcgccat cgttatcacc tgtaatttcg taaatcttca
     4081 cgcccaaccg cccaccggga acgacctgac cgcgcacaat attgatttca tcaaactgct
     4141 cgtcgtctat gagtagtccg gcatgcgtca gcgcatccag tggtgctttc aggatattgt
     4201 ccaggtcgcg gcggcgctta tccggtggtt ctgcagcgat cctgattgcc agccttccgg
     4261 acaggttcag tttcagtcgc tgctggcgaa caattagcgc cacatcacgg cgataacact
     4321 caccggcttt tgatacaaaa tatgtgctgc cacgacgtcg ccagtaggtg ttcaccgtcg
     4381 gcgggtaagg caaaacaaat tctatgcgtt cagtcattca tgctttccac ttcaggacac
     4441 ccgaatttct cgcgtgcatt aaaaaacgaa tcagcaacaa cagctggctg ccgtgtctgt
     4501 cttcaaaatc ttttaccccg gcgtgcagtt cgttatgaca tttacggcac agcggaataa
     4561 caaacaaatc atcagccttt gttcccatcc ctcccagtcc atgaccaatg atgtgatgcg
     4621 gatcatctgc ctgattaccg cacgtcatgc atttctgcgt ttttacccag cgcgtgtata
     4681 caggcatctc ttcacgttgt ggtttctggc gctggagata ctgagccggt gactccggat
     4741 caacggcaat actgaccacc gtcttttcct gtggcgggat ttgttgctgg tggacgtgag
     4801 acaacggcgc aagatttttt gtgcgctgct tcagtatgct ggtggcgctc tgctctcccg
     4861 gtacaatgtc gctttcgcgg tacactgagc ggattttgtc cgcacgtaac cccagagaac
     4921 gacgtaatac tgactctggg agtgcatccg ccacctgatt gcagaccgcc caccaggata
     4981 attcagccag agataattcc cgctcctgcg tgccattcat tgcatggcgt atgacgtcaa
     5041 tcatccatgc tgacaaattt tggtgagcaa gttgcccgag tgattcggag gtctggtcac
     5101 gcaactggtt gtcgcagtgc cagcacaaca ccatcgcgcc agcgccataa cggtgaatga
     5161 cggtttcact gtggtgataa tcgccgtgtg gccactggca ggatttaaca tggcgcaaca
     5221 gccagtcaga cagtgcacca gcgccgccag cagcacgaat cacacgctca ttgctgaaaa
     5281 acggcagtaa tgatttatcc tccgccagcg gctggcgaac ggcagggacg atgccggacg
     5341 gcagaccgcg catgcttttc ggttccggct ccacaagcac tcgagggtta tggaatacct
     5401 gcatggattc acggcctggt ttaaggacca ccaacccaag ttccggtacc ggaacaggtc
     5461 gaagtaatac ccgcacgtta cctccagatg cgttgctgga atgtgcggga cggacgcggt
     5521 gggcgctcgg agtacggcag cctgacgtag attatccagt gacgataatc gaggctgagg
     5581 gctttcctaa actcataccc acgtctgcgg tagttatgga tcagccattc ggcctgttct
     5641 tcagtgcagg gatcgtgctg ataccagtca tatttgaatg catgagaacg ccgcccgtgc
     5701 ctgctggcag gggcggcaga gttatccgaa ttgtaaaatt tggtatcgtg cgccatctgt
     5761 tttctctgct ggcgcagcag gtgccagttg ttcaggctga cggatggatt gtaaaccaga
     5821 acgaccagaa aaaacaaaac ccgccgaagc gggttaagtg cgggtgcgtt gaggatgcct
     5881 gacacatcag cggtggcgag ggatttctcc cccgccgggt ctcttactcc tcaggttcgt
     5941 aagctgtgaa gacagcgacc tccgtctggc cggttcggat tcgtacctcg cagaggtctt
     6001 tcctcgttac cagtgccgtc actatgacgg ttaaacagat gacgatcagg gcgattagca
     6061 tcgccttttg ctgcttcata gcctgcttct ccttgacctt tcggtccgta agaggctaat
     6121 ctctatgtgt cgcatagata tggcctcaga ttaatgttaa acgtcttgca ggacgcgtaa
     6181 tgttaactgg ggcttttctc tatctgccgt tggtgttcat gcccgagaca gatagcctca
     6241 agcacccgca gcaattctac ttaactaccg ttacctcgcc aatatgaaat caatcagaaa
     6301 ggcgatccat aagaacaaca gcaagacaat aaattgccat tacagccgca atagccagcg
     6361 cacatttgag aaccagcaca acaacctcct gtattggatg cacactagtc cctatcaata
     6421 tgaggccgtc tcgtcagtga atcaatacaa ctattgggta tagtttctct gattttttct
     6481 ggggaaatgg ggctcaagaa ccagtcacca ccagcacttc ttttaatacg caaagtccga
     6541 ctcaagctaa ccttccagtc cgctttgtgc gaagaacgga cttatctgta caagggaact
     6601 atccagcaat ggaaatcacc caaaagaaaa aactcctggt tcccctcgac aaccttcatc
     6661 ggggcaatta gcctcaatca acatttttat atctgtcagg tgacctcatt atgaaaaatg
     6721 atatcaaaac cctcgacctt aacttactta agacctttga tgcgttaatg gatgagggaa
     6781 gcgtgactcg ggctgcgcag cgactctccc tgacgcagcc cgctgtcagt ggcatgctta
     6841 ttcgattacg tgattatttt tgcgatcctc tctttgttcg caccagtcac ggtatggttc
     6901 ccactctccg tgctaaagaa cttgctatgc cggtaaaaca aattctcacc gatatcgcga
     6961 ttctgctgaa accaagtaaa tttgacccca tgacggcaga actaacttat accattgtcg
     7021 caacggacta tgcagttaag gctgttgttg tcccattgat ggctgcgctg aaacatcgag
     7081 caccgaatat taaaatctct gtgcggcctg tagacaataa acgaatatat cagcagttat
     7141 ctcagggaga agttgatctg gctttggtaa caccacaaac cactcccgga gagctgcacg
     7201 gcagagcact ttatgaagaa gattatgttt gcatagcacg acggaatcat ccgttgtcag
     7261 cgaattcaga aatgactctc gaacaatttt gcaaacagga acatattctg gtgtcttcag
     7321 aaggtagctt ctccggtgtt acagatgaag cactggctaa gctcagtctg acacgacgag
     7381 taggcatgtc agttaatagt tttcaagtag ttcctgatat attgcaagtg actgatatga
     7441 tcgctgttgt cccccaccgc atggtcctga ctaacaacga tctaatcata ctccctttgc
     7501 ccctgaaagt tccgggcttt accaagagta tggcctggca tgaacgaact catcgtgacc
     7561 ctagccatca gtggattcgg gctttatgcg tagaagttag ccaaggtact ggctcctgat
     7621 ataatttatc ttattaagat tataagtaat cataattcgc cttataactt cattgcgcta
     7681 ataatcactg ccatcgtgtt gcggtcgtaa cgcagcaaac ccagtacaat taactaccgc
     7741 aattggaggc atttatgaac gctattcact ggccagaagg ctttgttccg ggatttactg
     7801 ataactttgt gtctaatgaa atgatcatct ccggtctgaa catcaatgat gtttggccac
     7861 tccttagtca gccattacta tggccggaat actataaaaa ttcagcagat gtgcgctttt
     7921 ataacaataa aggtccggag ctggaaaatg gcgtgcgctt ttacttcagc acatttggat
     7981 ttccggttga agctcaggtc gtggaattta ttccacctac tgaaggtaaa cctgctcgtt
     8041 tggcatggca cggatggtcc ggtgaaaaag atactgtaca acgcctagat gttcatcatg
     8101 cgtggctact ggaggaactc tcgggtaaca gactccgtat cctgacacag gaaacgcaga
     8161 acggagttcc agcaaaagac ctggcaaata ctcgtccgaa cccaatgctc aatggtcacc
     8221 aggagtggct tgacggcctg attctggctg caaagaaata attactactt ttgcacaaag
     8281 tattactgca gttacatttc ccccaccatc ttcataatat tttcatcttt actattgaac
     8341 ttgagcacag gtaaatcctg tgctcctctc taagtgacag cgcatggcca tatttgcaaa
     8401 tgccgtttct ggcacaaaga aaactaccac gctagctcta ccctgcgcca tgaaaatgtc
     8461 aattcacatc tgaactaatg ctctttaatc tagtaacgtc taaaatacct aacatttcct
     8521 tgataaaatg ccagtatgcg ctgcataact tcgctctccc ggcactcgcg acagattata
     8581 ttcaggtgcc tgtcgtagcg acgtatttct ccatctggta atgaccagat caggtccggg
     8641 tcaaccatag atggtttctt cagattttcc cttgagagtt ttttgcgggc gttttgccag
     8701 tccttacgag cctgttcaga cgggaataac ccgtaaccag agttgtatat atcgccactg
     8761 gcaaccagct ctctggcgag aacgctgatc agatatcttg tcgcacctgt cttggattcc
     8821 agttgccgta gcgtctcgcg cccactccgg cgtactagct caacaacctg ccctttaatt
     8881 ttttctcgct cttcttgtgt aaaaactttt gccacaagcc ctcctgaaaa ttacctcatg
     8941 accagaaata cacacttacc ccctgaagcc cggtggaatt tcggtatccg gttcagaaat
     9001 atgattcaca caacgctggt tgttcgtacc gcttaccggg agcaaccagg ggttctcaaa
     9061 attccggtcc ggtcccaaaa acgtcgtcgc tcgctgaaca aattccgttc ccgttttccc
     9121 ggtagccgcc aggtatcttg cgtaacgcct cacgccatcc agcatggcct ctggtggcac
     9181 cccctcgcgt aatctggcct tccaggcact gaaagcggat ttcttcgggt ttgccccggc
     9241 acgcaacggg tattcccgcc agacctgttc gaacacatcc ggataatcca ctcgtcccac
     9301 aggctgcccg gttttttccg ggactacccg atcggcttcc cgctgaatgg cggaatcggc
     9361 ttcaggctgc tgcagttggt gtgattgctc cggccttgcg gtcatcacct gctgcacagc
     9421 gcccgaatcg gctttcagcg catacgctga atcggcttcc ggtgtcgtgc ctgctggctg
     9481 accaggattg acggtctgaa cattccctgc ctggttcgtg gcgtttttta cgccatggac
     9541 catagtgttt tgatcttctt tatctgtatc tttatctgta tctttatctg tcgtgactcg
     9601 tcgtgacatg tgcgtgacat ttcgtgacgc gccgtgacaa tcgccatttt gttcccgctt
     9661 tctttccctc tctcgctgcg ccctcttgcg ctctgcagga gattttgcgg tttgcgaaat
     9721 attgccgttg tcctctttca gcacctggcg tttttcccat ccagtgatta aatcaccatc
     9781 aagtacccgc ccctgcatcg tctgtaaaat tgaatcaatt acctcttctg tcacgtcgag
     9841 cgcacttgcc aaatcttctg tcgtgacatc aatgtgacct cgcgtgacat ttcgtgacgc
     9901 gctcaccagg aggtggatat acacagccat cactgttgcg attggctgtc ctgacaccct
     9961 ggaaattgtt cgccacttag gatcatttgg catgtcatgc cataatctga gccaggcgtt
    10021 agccatactc acctcttctg ataccgaatc tttttactca cgaattgccg gaagcgatcc
    10081 ggtataaata ttgtcagtca atgcacaacc acaatgtttc ccgccgggcc accacgattc
    10141 atctggttga aaccagcgat cgccactgcg acaaaatcat cagcgtctct caccagtcgt
    10201 tcccgcgtct ccaccagctc ccgaaaccag gctgaactgt ggctgcgcat tcgggccacc
    10261 agcagaggag gcattgcttt ttcgatcgct ggtaacaatg cctgaatttt tttaaccgca
    10321 tcaggggtgt ctttatccag ccaacggaaa attttctggg tattacgagc cagcgcttcc
    10381 ggatgcctgt cgtcatatag ctccgggaac gtcataccca gctcaaaata agcctgggtt
    10441 atttcagctg ccggaacttt ttcgccgtcc ggatgcgccc aggcattcat cgccatgcgg
    10501 atgtgttcat gcttgatttt catgaatcaa ctccatcaga taagcatgca ctacaatcac
    10561 cttcagcatg aactacatgc gtttgcccca aacgaatgcc gctagcatac tcaggccaaa
    10621 taagctccca atcatggggt cgtagctccg ccctacttac ttggccttcc gtcgcagatt
    10681 cgatcataag ggcgcgggtt ggagatatag ctgctcgtcc ggacgccatt tgcgataagt
    10741 aagatggcga tacaccaagt ctggccgcga atttcttagc atcaccaacc ctcaatgatt
    10801 taataaactc ttttaatgtc ataccttcct cggtttagtg ttttcttggg agtttagtgt
    10861 ttaataaacc attaagtcaa gtatttgctt gtttagtgat tactaaagat aattaccaca
    10921 tgcagaaaaa agaaattcgc cgtttacgtc tcaaggagtg gtttaaagat aaaactctgc
    10981 cacccaaaga gaagagctac ctatctcaac taatgagtgg gagggcctcg tttggagaaa
    11041 aggctgccag aagaatagag caaacatacg ggatgccgga agggtatctg gatgcggaat
    11101 acgcagaaca accggagatt tctccaccac atgcagggtt aacgtctaat caactggaat
    11161 tattgcagat tttttcagcc ttccctgagg atgagcaacg ccagataatc agcgagttaa
    11221 agcagaaaaa agaatcaatg gaagatctca tagcgagatg gattgcggcg caaaaatgcc
    11281 gccgcgccta agttataaaa ccggaggaaa catgaataga gccctttcac caatggtttc
    11341 tgaatttgaa accattgaac aagaaaacag ttacaacgaa tggctgcgtg cgaaagtagc
    11401 aacgagcctt gcagatccgc gcccagcaat tccccatgac gaagttgagc gcagaatggc
    11461 agaacgcttt gctagaatgc gcaaggaacg gagcaagcag taaaatgtta cccgtgttat
    11521 ggcttgaaag cgcagatacc gacctagatg atataaccag ttatattgct cgtttcgaca
    11581 tagatgcggc tgaacgctta tggcagcgat taaggggttg tgtgctgccg ttatccgaac
    11641 atccgtattt atacccacca agcgacagag tacctggctt gcgtgagatt gtggcccacc
    11701 ctaactatat aattctatac cgcgtaacaa catcaagcgt tgaagtagta aacgtgatcc
    11761 acgcaagacg ccagtttccc taactttaac taccaataga aacataacaa ccgcaacgac
    11821 tttaccaaaa gcgttgtgtt tgttacgccc cacggtttag tttttgcttg acttaagttt
    11881 aatgtttatt aaactaaaaa taccaaccca ccccgcccca cagaacgcag ggcaatactt
    11941 cgagttaccc ggcagtggtc aggggttaag tagccagccc gaggcgtaag aacatgacgg
    12001 cagggttcaa ctttaataac tatgcagcag gtttttgttc cgctaccccg gcgttaaggg
    12061 gaaacagagg gtttctcagt gggcgaagtc aaacatcaga atggaaggca tcccgggatc
    12121 ggcaaagaag cggcgatggc gctttatatt gacatcagcg ccattgccgg acaggtaaga
    12181 gttatcagag cggtaactaa gcggtatgcg cctttacttc agaaagtctc tggtgagtgc
    12241 accgaagata ttgtcaacga tttcgtcatc gaactgcgag gactcatctt cagttacaag
    12301 gtgaccacaa tttttgcaga tggctcccgc gaaactgtca gagccatgcg gtttaaagga
    12361 tgtgtcaaag acttcgccgc cacattctgg gcaagaaaac ttgattgtat tcataaccaa
    12421 tttcctctcg agtaacagat ccctcagagg ataccacctc gcctgacgtg gttaaaagca
    12481 ggcaacgcta accacaagga gccgacatgc agaaacgaga acccgtcatc atcgcgccag
    12541 actataccga tgatgaactt tatgagtgga tgcgccagaa aattaatgca gcgcaggatc
    12601 tgaaatgggc caatgaagcc agggctaagc aggctgaaaa tctgtccgct ctggagcagg
    12661 atatcaccaa tctggaaaaa gcagcggcat taagcattgc cagaatgatt acatacccac
    12721 gttaatagct aaccaacgag gctaataatg gcatttaaag atttaccaaa agaaatccag
    12781 acaattgctg caacgactct cggtgatagt ctggtgaaaa ttgacccggc atacaccaaa
    12841 aaagaaacca tcgataatat ggttcgtaat gtgcgcaatg ctttttctgg gctatatggt
    12901 tctgataatc aaaagcagga aagcgatgtt aataaacggg taatttctgt ttgcgtgaat
    12961 ggccatgttc tttcatcaat caaaacagaa acggcgacag tcttcgattg cctttgcatt
    13021 gtacagagcc ttgttgatgc cctgtttcgt tcagtgaatt tagaaaatga tgcaaatctg
    13081 cgagggcgca caatagcaca tccatatgca catactttag gctctgtgga tatcaaagat
    13141 cccacaaatc tttaatgaaa tagttaacgc gaattgtact tgctctttca gttgctttca
    13201 gaatacgcgt tgaaactgct ggcggtaatt tggtattcca tttattaaaa tcatgcccgg
    13261 gaaagtactc ttcgaaaata cttttaactg cagactcgcc tattgaaatg ctgcttacca
    13321 tgcgattttg ataaaggcat ttagcaataa gagttgattt taacattcac cctcctgagg
    13381 gttggtaatt aaggagttct ccacgggtga agtggagtgc gtgcgccgga cacgggtgaa
    13441 catccggcac tgacagttta ctgaaaggat atttctctga aaagtcagag cataacgcga
    13501 aagcgcgcgg cgaggttgct ggttcataga tagcctgtcg ttaaattttc gtcgaccgtg
    13561 cgcttccggt tgtggcactc cgcgaaatgg cgcggcggta agtatggcgg ggttattcct
    13621 tccccgttga ggacaccggg ttgtcaggtt aaccatacgc ttaagtgaca accccgctac
    13681 aacgccctct gttatcaatt ttctggtggc gtttggcggt atcagtttta ctccgtgact
    13741 gctctgccgc cctttttaaa gtgaattttg tgatgcggtg aatgcggcta agcgcacgcg
    13801 gaacagttaa aaccaaaaac agtgttatgg gtggattctc tgtatccggc attaattgtt
    13861 aactggttaa cgtcacctgg aggcaccagg caccgcatca acaaagttca tttgtaaaaa
    13921 tg
//
LOCUS       DAZFJL010000121        13868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFJL010000121 DAZFJL010000000
VERSION     DAZFJL010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.123"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(82..1620)
                     /gene="cadC"
                     /locus_tag="ACHJ8C_003616"
     CDS             complement(82..1620)
                     /gene="cadC"
                     /locus_tag="ACHJ8C_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO5553405.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTTTPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLETRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLQSLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLDRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(2237..2312)
                     /locus_tag="ACHJ8C_003617"
     tRNA            complement(2237..2312)
                     /locus_tag="ACHJ8C_003617"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2277..2279),aa:Phe,seq:gaa)
     gene            complement(2419..2994)
                     /locus_tag="ACHJ8C_003618"
     CDS             complement(2419..2994)
                     /locus_tag="ACHJ8C_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5553406.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQSAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(3031..4728)
                     /gene="dsbD"
                     /locus_tag="ACHJ8C_003619"
     CDS             complement(3031..4728)
                     /gene="dsbD"
                     /locus_tag="ACHJ8C_003619"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO5553407.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHELNLSWQIKDGYYLYRKQIRITPEQAKIADVQLPPGVWHEDDFYGKSEIYRERLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVTNNATPPSASVPQQEQPV
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQAHLNF
                     TQIKTVDELNQALAEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(4704..5042)
                     /gene="cutA"
                     /locus_tag="ACHJ8C_003620"
     CDS             complement(4704..5042)
                     /gene="cutA"
                     /locus_tag="ACHJ8C_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO5553408.1"
                     /translation="MLDDKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(5158..6459)
                     /gene="dcuA"
                     /locus_tag="ACHJ8C_003621"
     CDS             complement(5158..6459)
                     /gene="dcuA"
                     /locus_tag="ACHJ8C_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO5553409.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(6578..8014)
                     /gene="aspA"
                     /locus_tag="ACHJ8C_003622"
     CDS             complement(6578..8014)
                     /gene="aspA"
                     /locus_tag="ACHJ8C_003622"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO5553410.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            8351..8827
                     /locus_tag="ACHJ8C_003623"
     CDS             8351..8827
                     /locus_tag="ACHJ8C_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO5553411.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            complement(8843..10099)
                     /gene="yjeH"
                     /locus_tag="ACHJ8C_003624"
     CDS             complement(8843..10099)
                     /gene="yjeH"
                     /locus_tag="ACHJ8C_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO5553412.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSEQLLLAELGTLALVWYIGTHGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            10375..10668
                     /locus_tag="ACHJ8C_003625"
     CDS             10375..10668
                     /locus_tag="ACHJ8C_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO5553413.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            10712..12358
                     /gene="groL"
                     /locus_tag="ACHJ8C_003626"
     CDS             10712..12358
                     /gene="groL"
                     /locus_tag="ACHJ8C_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO5553414.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            12496..12849
                     /locus_tag="ACHJ8C_003627"
     CDS             12496..12849
                     /locus_tag="ACHJ8C_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO5553415.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(12899..13768)
                     /locus_tag="ACHJ8C_003628"
     CDS             complement(12899..13768)
                     /locus_tag="ACHJ8C_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO5553416.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSAMELR
                     DLLIALESRLHQKHKLDAAARLQYEQASDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDDQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGNEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT         3619 a         3320 c         3484 g         3445 t
ORIGIN      
        1 taccgcaaca tattatacca acgggaacat acaaaaactc aacaacaaat attcccgagc
       61 atgaatcaac ccgggagtta cttattctga agcgagaaat ttgtcgagat aaggtacaac
      121 ataaggaaca gaagtctgga atataccatt ttcaatccag taaagggtgt ttgcccctgg
      181 gcgtaaatta aaggcggtga gatatgcatc agctgcttcc cggttcatcc ccttcatttc
      241 ataaaccttg ccaagcaaca cataatttag ccaggacatt tcaagatcaa tgccagtatt
      301 tatcgcctga taagactcat ctgttttacc ttttaccagg gcactgaccg cttttatttg
      361 atatataatg gacaggttgt tcaattccgg cagtgtaaca atgttatcta tttctgtgtt
      421 aagtgctgct aattgttttt cgtctaaagg atgttgagaa tggcgcacga tatcaactaa
      481 tgctttttct gctctcgcgt aggtaaattc tggggatgat tgaacaatct cacctaataa
      541 ttcactggca cgatccaatg atttatcatc accatgcagt aaataatcat gtgcctgata
      601 aaaattagtt aataacgcac cacgatgcgg caaaatttgc tggagcatct cctgcattcg
      661 ttgtggccac ggttggttta acgcttttga taaactctgg agcaaatcat tttgaatcgc
      721 cagctgatta ccgttagtga tgacataacg tttatccagc atagttgaac catctgcatt
      781 gtctaccaat tttaccgaca taaagcattg ttgagcacgg tattggcgct gattaacaaa
      841 cgcaatagat aatgttttac cggaactgct cggttcatca atgttgtagt tgattttgtc
      901 atgcaccata aaggtggaga aggtgttaag tgatgtcgcc accaaatcac ccacgcctat
      961 cgcgtaagag agctgatacg gagaactcca gctgttacaa ctcttattaa ccatattaat
     1021 gtcaatatcg cgtggattga gcaaaatgcg cgatttactc ataggaagac gtgtttcaag
     1081 acttgaaaac gctaccagtg ctacacagat acctaacgac aacaggaaaa aaaaccatac
     1141 ccaaaaggta gtgaatcgtt tgcttttaac tggggattgt tcaggtggcg ttgtggtgtt
     1201 ttgaatgtta agactgtggg agggagaatc tgtggcagga accgcctctg gtataggggg
     1261 aggcgaagat agcattattt cctctccctc ttcttcgctg taccagataa ccggcaccat
     1321 taatttatag ccgcgctttg gtacagtagc gatatagaca ggactatctt catcattatc
     1381 ttttaatgac ttacgtagtt ctgagatact ctgcgtcaca acgtgattgg tgacaatact
     1441 tctcttccag acattatcga taagttcatc cctgctaagt acttcgccac tgtgttgagc
     1501 aaagaaaacc agaagatcga ttaatctcgg ctcaagggta agttgacgcc cattgcggct
     1561 aatttggttt atggacggag taacaagcca ttcgccaacg cgaactacag gttgttgcat
     1621 aatagaaact cattcgaaaa gggaatgatg caatgataat tgccataacc tattttttcc
     1681 atctatagat gggtttattt acatattatt ggtgaatgca ggacgttatt tttacccgcc
     1741 ataaacttct tgattacata gtattacgaa aggattttac tgagaaccag aagtaatttt
     1801 ccttaccatc aaaattcatc atctttgcca aagaaaaatg ttcagaaaat aatccatgaa
     1861 aaattgtccg gagcgcttac tattttaatg gattgttagt ctttgcatga gcaaacggac
     1921 tgatgcattt ctctttgttc tcattcagaa aatctcatca gttggcgttc tgcccgatgt
     1981 tgtgctttat gggggcgtta cgctcctgat gacgtcattt gacgttcaac agcatcacgg
     2041 ggccgcacga catttcacgt cagtcagcgc tatagctcag aaacaaattt tcccgaattg
     2101 ggatatgccc gcaaattgct ggtgatgtgg ggagaatctg gttgagttcg gtagaattga
     2161 tttggcggca ggacgcttaa attgtggcgt cctgaaacga aaaacggacc tccgtggagg
     2221 tccgtttata tgaatttggt gcccggactc ggaatcgaac caaggacacg gggattttca
     2281 atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc gtaatctgca
     2341 catctcgtca acctaatttc agtaaaagcg attcaactgc ttaagattgc ggcaaatcgc
     2401 ttcttttctg tgtttctgtc aggtcagtgc accgccctgg cggcagcggg cgaagcgcag
     2461 gatatcttcc gccagccggt gtgcggtgtc gacatccgcc tggctacgat ttaccagcat
     2521 tcggctcagg cacccttcca gcaccagttc catctgcttt gctaccatcg ccggatcgtc
     2581 cacttccagc gtggttaaca gttcgtgggt aaagtcgtaa gccgcgcttt tttgctgatc
     2641 ggccaattga tgaatagggt ggccaggatc gggataaaac gtacaggcag cgataaacag
     2701 acagcccgga tagcggttgt ttttaacgca ctccgatagc gcctgataac gcgccagcag
     2761 tttttgctcg gcggattgcg tctcgtccag catcagctga cgacgccaga catctatctg
     2821 ttggctaaga tagcgcaggg cgtcgtagag gatcgcctct ttgtctggcc agaagcggcg
     2881 cagctcgtcc agtggataat ccacacgttc agcaaccatc tccagcgtgg tgttggcaat
     2941 cccttgtaat tctaataatt tcagggcttc tcccagtaca tcttcacgtt gcacgctatt
     3001 ttcctccgtc tttcccactg caagtgtcgt tcacggttgg cgatcgcgca aatgtgcgct
     3061 gaaggtttca gcatccataa agcccgtgac gcgcgcttgt ggatgctcct ggccttgtcc
     3121 gtcaaaaaag agaattgtcg gcaggccaag gacattaaga tgctttaaca gcgccacatc
     3181 ttgtgcgtcg ttggcagtga cgttggcctg aagtaagacc gtgtctgcta acgctttttg
     3241 cacctgcggg tcgctgaagg tgtatttctc gaactcttta caggcaacac accagtcggc
     3301 gtagagatct aacatcaccg gtttgccttt ggcttcagca agtgcctgat ttaactcatc
     3361 taccgttttg atttgggtaa agttgagatg cgcctgagtt tgcgcggtat gcgtcgcgcc
     3421 aaatgcccaa tcctggagtg ggcgtacgct aaccaatgct gccgccagca ggattatttg
     3481 caccacgcgc atccagccgc gtttggcctg taagctggtg ataaaggccc agccaaagaa
     3541 cgcgacacca agcgccgacc acaagcgtaa tccccatata tcaccaatca ctcgctccag
     3601 caggaagacc ggcagtgcga ggattacaaa accaaatgcg gttttgacct gttccatcca
     3661 cgggccgctt ttcggcagca ggcggttacc aaagacggta attagcatca gcggcaggcc
     3721 catgcccagc gcatagagat aaagcgtgcc gccgcccagc cacatgtttc cgctttgggc
     3781 gatatacagc agaatcgcgc taagcggtgc ggtggtgcat ggtgaacaga tcagcccggc
     3841 aatcgccccc ataacaaaca caccgccagg cgagccacct tgttggcgat tgctcattag
     3901 cgtgaggcgc gtttgcagcg aagaggggag ttgcagggta aacaagccaa acatcgacat
     3961 tgccagcaag gtaaagacga tagcgaggcc gataagcacg taaggatgct gtagcgctgc
     4021 ctggaactgt aaccctgcgg cggcaaccac cagacccagc gccgtgtagg tgagcgccat
     4081 gccctgcaca taaataaaag tcagcaacaa tgctctggcg gtggatagcc gctgtttacc
     4141 acccagcaca ataccggaaa ttagagggta catcggcagt acacatggcg tgaaggcgat
     4201 accgatacca atcaacaacg cccagagcgc ggaaaagggc aattgcgcga cgggctgctc
     4261 ttgctgcgga actgatgcag acggtggcgt cgcgttgtta gtaaccactt cgcttaacgg
     4321 aacggttttg gtttctggcg ggtaacaaaa accggcgtca gcacagccct ggtaggtaac
     4381 agtcaatgtc gcaccagcac ttgcctggtt gatggtcaca ggaagcgtca gccgctcgcg
     4441 gtaaatctcg cttttgccgt aaaagtcatc ttcatgccag acgcctggcg gtaactgcac
     4501 gtcggcaatt ttcgcttgtt ccggcgtaat gcggatctgt ttacggtaaa gatagtaacc
     4561 gtctttgatc tgccagctca gattaagttc atgttggttt tgctgaaaat caaaagcaaa
     4621 ggcttgatcc gcggggacaa attgtgagcg tcccggcgcg tcgaataatc cggcaaaaac
     4681 ggaagtgctg caaagtagca ggatcagcgt aaagatgcgt tgagccatga gaggtaatct
     4741 gtgtctccgt gtgtaacagg taaaaccaga agctccgggg tttgatacgg atgatgagac
     4801 ttcaggcatt ccagcagtgc ctgctggtga gatacggtgg tttttaaaat catctgcact
     4861 tcgtattcct gttccagttt accttcccag taatagagag aggtagcgcc aggaatcaag
     4921 gtcgcgcagg ccgccagttt ttctgccagc actttggcgg ctaaatcctg ggctgtcgct
     4981 tcatccggtg cggtacacag cactacgaca gacgcggtat tcgaactttt atcatcaagc
     5041 ataaacacct cgcaagaaca gatggaaccg caagagagag gtcactatac aacggacgag
     5101 gaaaggatgt tagtcggcgg gaaagaaagc ggggcgtgaa cgccccgcga tttgtcatta
     5161 cagcatgaag ctacccagca cgaagccgaa gcaaacggcc agggcaacac ccagagtacc
     5221 tgggatgaag aacggatggt tgaagacgaa tttaccgata cgagtggtac ctgtgtcatc
     5281 catctgtacc gcagcaacca gcgtcggata ggtcggcaga atgaacagac cagacacggc
     5341 tgcgaaagaa gctacagcgg tcagtggaga aacgttcagc gccagagcca tcggcatcag
     5401 tgcttttgcg gttgcagcct gagagtacag cagagcagaa gcaaagaaga agatgacggc
     5461 cagcagccac ggatgaccct gaatcacttc accagcggta tctttgatcc agtcgatgtt
     5521 gttggaaacg aaagtatcgc ccagccacgc aacacccagg atacaaatac aggcgctcat
     5581 acctgctttg aaggtgctgg agttgagaat gttgtcggta tcgactttac agataacggt
     5641 ggtcagagtt gcaacgctga gcatgatgat caggattgcg ttggtggtgt tcatcagcgg
     5701 tttttcaacc agacccatgc ttgggctgtt gatgattgca tagataacca cgccaactac
     5761 gcccagcagg aacagccaga cggacgtttt tgcaccggat ttgatttcaa tctgcttttc
     5821 accgcgcagt tcaaccaggc cctcttccag acgcttgcga taaatcggat cgtcagagag
     5881 tttggagttg aacagcatcg tgaccaggaa ggacatcacc agaaccgcca gcagggtgga
     5941 cgggatgacc acggagagca gatggaggta gctgatgcca tgaccttcca tcacggaaga
     6001 catgtaaacc actgccgctg agattggcga tgcggtgatc gcaatctgcg cggataccac
     6061 tgcagtagac agcggacggc aaggtttaac gccttgttcc ttcgcaactt cagcgataac
     6121 tggcagtgtc gccagagaga tgttgccagt accagcaaag atagtcagga aataggtcac
     6181 gatcggtgcg aggatcgtga tgtatttcgg gttacggcgc agcagctttt ctgtctgatg
     6241 aaccagatag tccagaccgc cagcaacctg catggcagaa atagcggcga taaccgccat
     6301 gataatggaa atgacatcga atgggatgtt accgggttta acgccaatag cggcaagaac
     6361 cagcaccccc aatccgcctg caaaaccaat acctattccc cccaatctgg cgcccaagaa
     6421 gatcgccagc aaaactatga tgagttctac aactagcata ttagccttcc ttgttttttt
     6481 aacaagttga tattaaattg ttatttttaa gttactgctc acaagaaaaa aggcacgcca
     6541 tctgacgtgc cttctttatt tgtactaccc tgtacgatta ctgttcgctt tcatcagtat
     6601 agcgttttgc tttgtaagcc gggtgcatca gattctgtac ggagaaaata tcgtcaagtt
     6661 ccgcttcagt caacagaccg cgttccagaa cgacttcacg tacactctta ccggtttcgg
     6721 cacagatttt acccacgatg tcaccgttgt ggtgaccgat gaacgggttc aggtaagtaa
     6781 cgataccgat agagttgtaa acgtaacctt cgcacacttc tttgttagca gtgatgccgt
     6841 taatgcattt ttccagcagg ttgtagcaag cgttggtcag aatgtgaacg gattcgaaca
     6901 tggcctggcc aatgactggc tccataacgt tcaactgcag ctgacctgct tctgctgcca
     6961 tggtaacagt ggtgtcgtta ccgatgactt tgaagcatac ctggttaacc acttccggaa
     7021 caaccgggtt tactttagct ggcatgatgg aagagcctgc ctgcagttcc ggcaggttga
     7081 tctcgttcag gccggcacgt ggaccagaag agagcaagcg caggtcgtta cagattttgg
     7141 acatcttcac agccaggcgt ttcagcgcgc cgtgaaccat aacataagcg ccgcagtcgg
     7201 aggtcgcttc gatcaagtct tcagccggta cgcatgggaa gccagtgact tcagccagtt
     7261 ttttcactgc cagcggagag tactctttcg gcgtgttcag accagtaccg attgctgttg
     7321 cgccaaggtt aacttccagc agcagttcag cggtacgttg gatgtttttc acttcttctt
     7381 tcagcaggat gctgaaggcg cggaattcct gaccgagggt catcggtact gcgtcctgca
     7441 gctgggtacg acccattttc aggatgtcct ggaattcgac agctttacgt tcaaagcctt
     7501 cacgcagttg gttaatcgca tctaccagtt taatcaggga agagtaaact gcgatacgga
     7561 aaccggtcgg gtaggcgtcg ttagtagact gacatttgtt aacatggtcg ttcgggttca
     7621 ggtactgata ttcacctttc tggtgaccca tcagttccag accgatattg gccagcactt
     7681 cgttggtgtt catgtttacg gaagtacctg cgccgccctg gtagacgtct accgggaact
     7741 gatccatgca ttttccgttg ttcaggactt catcacatgc ggcaatgatg gcattcgcta
     7801 cacttttagg aatggtttgc agctctttgt ttgccatagc tgcggctttt ttaaccatta
     7861 ccataccgcg aacaaattca ggaatatcac tgattttgtt gttgctgata tagaagtttt
     7921 caatcgctct cagagtgtga acaccatagt aggcatcagc tggaacttcc ctggtaccca
     7981 acagatcttc ttcgatacga atgttgtttg acatgtgaac cttctttttc aagctgccaa
     8041 tgatttgctt taaacacaca gaatatatgt ggtttcgaat gtttttcggc cgacgattat
     8101 cccctgcatc gaccgaatac ccgagatcat atgctgcttg aggatttcta ccgtaacctg
     8161 gatcacttta agtgtcggtt tttacccctt aattattaat ttgtgaaatg gatcaccgct
     8221 ttgggattac taccaaaaat agttgcgcaa acatcttgaa attttgctaa tgaccacaat
     8281 ataagctaaa cgcgattcgc aacccattca ggtagccggg tttaaccggc tgctattaca
     8341 ggagaaacct ttgcgctggt taccttttat tgccattttc ctttatgtct atattgagat
     8401 ttcaatcttt attcaggttg ctcatgtatt gggggtattg ctgaccctcg tgctggttat
     8461 attcacgtca gttatcggta tgtcactggt acgtaaccag ggctttaaga atttcgtgct
     8521 gatgcagcaa aaaatggcgg cgggtgaaaa cccagcggcg gagatgatta aaagtgtttc
     8581 gctgatcatt gccggtttgc tgcttttatt accgggcttt ttcaccgatt tcctcggttt
     8641 gcttctttta ttaccgccgg tgcaaaagca tctgacagtg aagttgatgc ctcatctgcg
     8701 tttttcccgt atgccaggcg gcggttttag cgccgggacc ggtggcggta atacttttga
     8761 tggtgagtac cagcgaaagg atgatgaccg cgaccgcctt gatcataaag acgatcgcca
     8821 ggattaatgt cgaaacgccg gattatgtgg ttatgccatt ttccggcgtt tttcgttttg
     8881 gcagaaacag ccataacccc gccagcatga tcagtgcgta gagacttttc cagccgacca
     8941 ttgccagtaa cagaacgcat aatagcccgc caaccactgc cagtagtcga taacgtcctt
     9001 gcaataattt acagcctgcc agcatgcata acagataaat cataataaag atgccattgg
     9061 cataaataat aagagcgtcc agattgatct ctaaagcatg aatcaccaac gtgctcacca
     9121 cgcagcagcc gagcaccgca ttgagggcat tattcggaat atggcgagaa gagaggcgtg
     9181 ccaggtagtg gtcaggatta tgttgcgcct gcgaccagac caggcgggcg aagctctgta
     9241 tataaatgtt gagactggca aagcaggcca gatagccaat tacgcaggca atccataacg
     9301 ctcctacacc gaacaactgc actacaattt tgggaagcga tgctgccgcc gccatttgtt
     9361 caccataggc gtcgaagtgt aagacgacta ccgtacagcc ccaatagact aatcctgcca
     9421 gcagcaggcc aatcatcaaa gcacgaggaa aatcacgctc tggattttta aattccgagg
     9481 caagatgggc aaatgcttcc agaccgacaa aacaccagaa catcactgat aacgcagcaa
     9541 ataacccggt aagttcgata tttcctggcg cagggaaggg gatattcgca ggtttgatat
     9601 cgcccgccca ccagatagcg acaatcagtg cgacgataag tccggcaata actgtttgta
     9661 gattagcact ggaacttgca ccgtgagtac cgatatacca caccagcgcc agcgtaccga
     9721 gttctgccaa caacagttgc tcgctatgcc agccaaacat tgcctggccg aacccggcgg
     9781 caatttgtag cgcggcaggc aaacccacgg gaatgaccga taaaaacaac cagccggtga
     9841 ctcgctcaag ccgcgaacca aacgccatac cgacgaagtg cgcgacaccg cctgcgctgg
     9901 gatagtggcg acccagaatc gcaaacacaa tcgcaatcgg gaacactaag ataatcaaaa
     9961 cgggccacgc ccacaggctg ttattgcccg ctaccagcgc agctaacgca ggaacggcaa
    10021 acacgccagt gcctaataat gacgtcgata gcaggccaat gccctgggcc agccccagtt
    10081 cttgtttgag tccactcatg ggttgatgtc cgattgcgcc caaattatgg gcaactgcgt
    10141 agattttcga tggtagcaca atcagattcg cttatgacgg tgatgaagaa atagcgatga
    10201 aatgtgaggt gaatcagggt tttcacccga ttttgtgctg atcagaattt tttttctttt
    10261 tcccccttga aggggcgaag cctcatcccc atttctctgg tcaccagccg ggaaaccacg
    10321 taagctccgg cgtcacccat aacagatacg gactttctca aaggagagtt atcaatgaat
    10381 attcgtccat tgcatgatcg cgtgatcgtc aagcgtaaag aagttgaaac taaatctgct
    10441 ggcggcatcg ttctgaccgg ctctgcagcg gctaaatcca cccgcggcga agtgctggct
    10501 gtcggcaatg gccgtatcct tgaaaatggc gaagtgaagc cgctggatgt gaaagttggc
    10561 gacatcgtta ttttcaacga tggctacggt gtgaaatctg agaagatcga caatgaagaa
    10621 gtgttgatca tgtccgaaag cgacattctg gcaattgttg aagcgtaatc cgcgcacgac
    10681 actgaacata cgaatttaag gaataaagat aatggcagct aaagacgtaa aattcggtaa
    10741 cgacgctcgt gtgaaaatgc tgcgcggcgt aaacgtactg gcagatgcag tgaaagttac
    10801 cctcggtccg aaaggccgta acgtagttct ggataaatct ttcggtgcac cgaccatcac
    10861 caaagatggt gtttccgttg ctcgtgaaat cgaactggaa gacaagttcg aaaacatggg
    10921 tgcgcagatg gtgaaagaag ttgcctctaa agcgaatgac gctgcaggcg acggtaccac
    10981 cactgcaacc gtactggctc aggctatcat cactgagggt ctgaaagctg ttgctgcggg
    11041 catgaacccg atggacctga aacgtggtat cgacaaagca gttaccgctg cagttgaaga
    11101 actgaaagcg ctgtccgtac cgtgctctga ctctaaagcg attgctcagg ttggtaccat
    11161 ctctgctaac tccgacgaaa ccgtaggtaa actgatcgct gaagcgatgg acaaagtcgg
    11221 taaagaaggc gttatcaccg ttgaagacgg taccggtctg caggacgaac tggacgtggt
    11281 tgaaggtatg cagttcgacc gtggctacct gtctccttac ttcatcaaca agccggaaac
    11341 tggcgcagta gaactggaaa gcccgttcat cttgctggct gacaagaaaa tctccaacat
    11401 ccgcgaaatg ctgccggttc tggaagctgt tgccaaagca ggtaaaccgc tgctgatcat
    11461 cgctgaagat gttgaaggcg aagcactggc aactctggtt gttaacacca tgcgtggcat
    11521 cgtgaaagtt gctgcggtta aagctccggg cttcggcgat cgtcgtaaag ctatgctgca
    11581 ggatatcgca accctgactg gcggtaccgt aatctctgaa gagatcggta tggagctgga
    11641 aaaagcaacc ctggaagacc tgggtcaggc taaacgtgtt gtgatcaaca aagacaccac
    11701 caccatcatc gatggcgtgg gtgaagaagc tgcaatccag ggccgtgttg ctcagatccg
    11761 tcagcagatt gaagaagcaa cttctgacta cgatcgtgaa aaactgcagg agcgcgtggc
    11821 gaaactggca ggcggcgttg cagttatcaa agttggtgct gctaccgaag ttgaaatgaa
    11881 agagaaaaaa gcacgcgttg aagacgctct gcacgcgacc cgtgctgcgg tagaagaagg
    11941 cgtggttgct ggtggtggtg ttgcgctgat ccgcgtagcg tctaaactgg ctgacctgcg
    12001 tggtcagaac gaagaccaga acgtgggtat caaagttgca ctgcgtgcaa tggaagctcc
    12061 gctgcgtcag atcgtcctga actgcggcga agaaccgtct gttgttgcta acaccgttaa
    12121 aggcggcgac ggcaactacg gttacaacgc agcaaccgaa gaatacggca acatgatcga
    12181 catgggtatc ctggatccaa ccaaagtaac ccgttctgct ctgcagtacg cggcttctgt
    12241 ggctggcctg atgatcacca ccgagtgcat ggttaccgac ctgccgaaaa acgatgcagc
    12301 tgacttaggc gctgctggcg gtatgggcgg catgggtggc atgggcggca tgatgtaatt
    12361 gccctgcacc tcgcagaaat aaacaaaccc ccgggcagaa atgtctgggg gtttttcttt
    12421 tggtcatctt tctagtataa gattcagaca cggacgacgc gagtggcgtc cagctcattg
    12481 attatgggga ataacatgca cgtaaaatac ttagcaggga ttgtcggtgc cgcgctactg
    12541 atggcgggtt gtagctccag caacgaattg agtgctgccg gtcagagtgt acgcattgtg
    12601 gacgagcaac caggtgcaga gtgtcagctg attggtactg ctacaggtaa gcaaagcaac
    12661 tggctttccg ggcaacacgg agaagaaggc ggttctatgc gcggcgcagc aaacgatctg
    12721 cgcaaccagg cggctgcaat gggcggtaac gtgatttatg gcatcagcag cccgtcgcag
    12781 ggaatgttgt ccagttttgt cccgacggat agccagatta tcggtcaggt ttataagtgc
    12841 ccgaactgat gtgatccaga ggctggatac ttccagcctc gtagtaaaac agatgagatt
    12901 atttcgcctg tggtgcagtt ttgctggtgg attgaacacg taagtgatgc agcgcgcgta
    12961 agcattgatc ctgagttagc ggttgggcgt tatttgccgc tacggtgcga acatagacct
    13021 gacatggcac acccgcgagt tttttcagtc gggggctgaa atcaagcatt cggcgagtga
    13081 tcgcctccac ttctttaccc ggcgttgctt cacgcgtttt caccactgcg ccgctaaatt
    13141 gttcattgcc actttcatct ttaaagcggt aaagcaccgc cagataatgg ttgaacaagt
    13201 tatgtttcca ttccggttgg gaataggtgt catattccag attgccattt tcctggcagt
    13261 caacgtcgta taacggcagg aaatctaaca gtgaggtaat acgtaatgcc agttcacgca
    13321 tttccgcatt attaattgca tgaatgattg ctcgcgccag catttcaatc tgcaactcat
    13381 tgacgactaa ctcacatttg ctaccgtcat gtagcgttaa tacgaaggta agattttcgc
    13441 cctgatcatc ggtaagttcc agcgtgttaa cccgacgatt aatatcggca tttttaagct
    13501 catcaaccag aatttctggg atattttctg ccattttctt aatgacttta tcgctagctt
    13561 gttcatactg aaggcgtgca gcagcatcca gtttatgctt ttgatgcaga cgactttcca
    13621 gcgcaatcag aaggtcgcgc aattccatgg cagacataaa gaacaacgat tctttattgc
    13681 gaggttcttt aattttcagc gccagtgcaa tgaaattatt actcttgcga ataacaccgg
    13741 tattaacacc cttgatgctt atggccatgt aggttctccc taaccatttc tcaattaaat
    13801 aattaatttt agtttataag ccagataaat gggcttggta gtaatagttg ttaaaataac
    13861 ataaatag
//
LOCUS       DAZFJL010000122        13850 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFJL010000122 DAZFJL010000000
VERSION     DAZFJL010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.124"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(94..846)
                     /gene="tcyN"
                     /locus_tag="ACHJ8C_003629"
     CDS             complement(94..846)
                     /gene="tcyN"
                     /locus_tag="ACHJ8C_003629"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO5553417.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGVAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(843..1511)
                     /gene="tcyL"
                     /locus_tag="ACHJ8C_003630"
     CDS             complement(843..1511)
                     /gene="tcyL"
                     /locus_tag="ACHJ8C_003630"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO5553418.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1526..2512)
                     /gene="dcyD"
                     /locus_tag="ACHJ8C_003631"
     CDS             complement(1526..2512)
                     /gene="dcyD"
                     /locus_tag="ACHJ8C_003631"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO5553419.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNTQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPSYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2617..3417)
                     /gene="tcyJ"
                     /locus_tag="ACHJ8C_003632"
     CDS             complement(2617..3417)
                     /gene="tcyJ"
                     /locus_tag="ACHJ8C_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5553420.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3505..4056)
                     /gene="fliZ"
                     /locus_tag="ACHJ8C_003633"
     CDS             complement(3505..4056)
                     /gene="fliZ"
                     /locus_tag="ACHJ8C_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO5553421.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPSTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSQPASDIY"
     gene            complement(4102..4821)
                     /gene="fliA"
                     /locus_tag="ACHJ8C_003634"
     CDS             complement(4102..4821)
                     /gene="fliA"
                     /locus_tag="ACHJ8C_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO5553422.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5140..6777)
                     /locus_tag="ACHJ8C_003635"
     CDS             complement(5140..6777)
                     /locus_tag="ACHJ8C_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFO5553423.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLSGFNVNGSADKASVAATADGMVKDGYIKGLTSSDGSTAYTKTTANTAA
                     KGSDILAALKTGDKITATGANSLADNATSTTYTYNATSNTFSYTADGVNQTNAAANLI
                     PAAGKTTAASVTIGGTAQNVNIDDSGNITSSDGDQLYLDSTGNLTKNQAGNPNKATVS
                     GLLGNTDAKGTAVKTTIKTEAGVTVTAEGNTGTVKIEGATVSASAFTGIAYSANTGGN
                     TYAVAANNTTNGFLAGDDLTQDAQTVSTYYSQADGTVTNSAGKEIYKDADGVYSTENK
                     TSKTSDPLAALDDAISSIDKFRSSLGAIQNRLDSAVTNLNNTTTNLSEAQSRIQDADY
                     ATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            7043..8449
                     /gene="fliD"
                     /locus_tag="ACHJ8C_003636"
     CDS             7043..8449
                     /gene="fliD"
                     /locus_tag="ACHJ8C_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO5553424.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIANSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDSKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            8474..8884
                     /gene="fliS"
                     /locus_tag="ACHJ8C_003637"
     CDS             8474..8884
                     /gene="fliS"
                     /locus_tag="ACHJ8C_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO5553425.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            8884..9249
                     /gene="fliT"
                     /locus_tag="ACHJ8C_003638"
     CDS             8884..9249
                     /gene="fliT"
                     /locus_tag="ACHJ8C_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO5553426.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9327..10814
                     /gene="amyA"
                     /locus_tag="ACHJ8C_003639"
     CDS             9327..10814
                     /gene="amyA"
                     /locus_tag="ACHJ8C_003639"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO5553427.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10848..11261)
                     /gene="yedD"
                     /locus_tag="ACHJ8C_003640"
     CDS             complement(10848..11261)
                     /gene="yedD"
                     /locus_tag="ACHJ8C_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO5553428.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMFDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11448..12653
                     /gene="yedE"
                     /locus_tag="ACHJ8C_003641"
     CDS             11448..12653
                     /gene="yedE"
                     /locus_tag="ACHJ8C_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO5553429.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12650..12883
                     /gene="yedF"
                     /locus_tag="ACHJ8C_003642"
     CDS             12650..12883
                     /gene="yedF"
                     /locus_tag="ACHJ8C_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO5553430.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12990..13658
                     /locus_tag="ACHJ8C_003643"
     CDS             12990..13658
                     /locus_tag="ACHJ8C_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO5553431.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     NRRPLVLSPEAAREWMRQEVGGKEASEIAASGCVTANQFTWHPVSCAVGNVKNQGAEL
                     IQPV"
BASE COUNT         3364 a         3592 c         3443 g         3451 t
ORIGIN      
        1 ttaatgagtt atgctgattt gttaagcagt tttatcacgt ttgaaatggc gtcccgcccc
       61 gactggtgaa tcgtcggggc tgattttttc ttattattgc agcagaaact tctccaggaa
      121 ctggcgggtg cgaggctgct gaggatcagc aaataacgct tttgctaccc cctgctcaac
      181 tatccgcccc tggtccataa aaatcgcccg gtccgcaaca tcccgggcaa agctcatttc
      241 gtgcgtcaca atcaccatcg tgcgcttttc ctgcgccagc tgacgaatgg tgttcaggac
      301 ttcgcccacc agctctggat ccagcgcaga cgttggctca tcaaacaaaa tcacttcagg
      361 acgcattgcc agagcacgcg caatcgcaac gcgctgctgt tgaccgccag acaaacgacg
      421 tggataactg gtttctttac cggccagccc aactttcgcc agcagctcgc gagcgcgcgc
      481 cgtggcctct tctttcggtt cgcctttgac gatcaccggc ccttcaataa tgttttccag
      541 caccgtacga tgcggaaaca gattaaagtt ctggaagaca aacccgacgt gctgacgcaa
      601 ctggcgaatc agcgattttt gctgacttaa tgaacgtgca gtatcaatag tgatatcgcc
      661 aacggtgatg gttcccgctt ccggttgttc cagcagattt atgctgcgta gcaacgtggt
      721 tttgccggaa ccactcggac cgataattgc cactacttcg ccaggcttca cctcaaggtc
      781 gataccatgc agcaccgtct gaccgtggaa ttttttcacc aggttcttaa cttcaatggc
      841 actcatttgg gttccctctc ctggcgatta agttgattct caaaatggtt ctgcaacgtc
      901 gataacacag tcgccataat ccagtagatg agcgaagccg ccaggtacat ggtgaacacc
      961 tccagcgtgc gcgaggtaat caactgcgcc tgacggaaca gctccggcac ctggattgtc
     1021 gcggccagcg acgtatcttt taccaggctg ataaagctgt tcgacagcgg cggcaacgcc
     1081 acccgcgccg cctgtggcaa aatcgcgcga cgcatggtct gccatggcgt cataccaata
     1141 ctggctgcag cttcccactg acctttatca atagaagaaa tggcggcgcg cagcgtttcg
     1201 gcagcatagg cggcagtgtt cagcgacaga ccaatcattg ctgacggaat gggatctaat
     1261 tcgataccaa actgtggcag tccgtaatag atcataaaca gttgtgcgat gagcggcgta
     1321 ccacgaaaaa tagagatata aaaacgcgcc agccagcgca cgggccagat tggcgacagg
     1381 cgcatcagcg cgagaataaa cccgagcagt aagccaaaaa acatgccgcc gatactgagt
     1441 tgcagcgtat acccagcccc tttcagcagg aacggcaagg aatcaataac cagttgtata
     1501 ctttcttgca tgagcgttcc cggagctaaa cgtggggatg ataggcgaac agcgcaggcg
     1561 cgccgccggt atgaataaac agaatcggcc cttcatcttt gaagcgtttc tgactgatac
     1621 cgtcaatcag ccccgccatc gcttttccgg tatacacagg atcaagcaga atgccttcca
     1681 gccgcgccag caatttcact gcttccatgc cttcgtcgtt cggcacgccg tagctaggtg
     1741 caaaatagtc atcccagagt aaaatttccg ctgatgcggt cagctccagt tctttcgcaa
     1801 tcgcctgttg gaggttaacc actttcggca attgatcggc aacggaacgc gacacggtca
     1861 cgccaatcag ttcgctttca ggcatcaggt gttccagccc aacagccagt ccggcgtgag
     1921 ttccggcact gcccgacgcg accaccaccg acgaaatatt aactgcccct tcacactgtt
     1981 gcgcaatttc cagcgcactc tccacataac ccagcgcgcc caaagcatta gaaccgccaa
     2041 ccggaatgac atacgggcga aagccttgtg cttcgactcg cgtcgccagc tcttccagtt
     2101 gggtattggg atcggtcagt gcgtcgcaca tttcaatctg ggtattgaac agatccagca
     2161 acaaacgatt gccgttggtt aaatagtttt ctgcggttgt gccaatagga ttttccagca
     2221 gcgccacgca gtgcagaccg agtttcgccg caactgcggc agtctggcgc acatggttag
     2281 actggatcgc cccggcagta atcagcgtat cggcaccttc acgcagagca tctgccgcga
     2341 gaaattccag cttacgtaat ttattgccgc ccattgccat gggagtgacg tcatcccgtt
     2401 tgatgaaaat ttcccgtcca agataatcag aaaagcgcgg cagatattcg agcggcgttg
     2461 gcgcgccgat aaactccagc cgtggaaaac gggttaaatt atgcagtggc ataacagcct
     2521 ccgatgtgtg ttgttgtgat tttcttatta tgcacgctga aaacgcgtaa ataaaaaagg
     2581 cgctagtgaa agcgcccttt tttgtcatta tgctgattat ttggtcacat cagcaccaaa
     2641 ccatttttcg gaaagggctt gcagagtgcc gtctttttgc atttccgcaa ttgcatcatt
     2701 cactgctttc agcaggtctt catttccttt acgcaacgcc acgccagact cctgacggga
     2761 gaatgcttca ccggttactg ccagcgtatc gttggttttc ttcaccagat ccagcgccgc
     2821 cagacgatca acgaggatcg catcgatacg gcctacgcgc agatcctgat atttggtcgg
     2881 gtcatcatca taggtacgca catcgacacc ctgaacattc tgacgcagcc actcttcata
     2941 gttggtgccc agaccgacac ccactttttt gcctttcaga tcagcagccg ttttaatggt
     3001 gccttcgtta ccttttttca ccagcgcctg aataccagaa atggtgtacg gggtagagaa
     3061 atcgtatttt ttcttgcgct cgtcagaaat ggtgacctga ttaatcacca catcaatgcg
     3121 tttagagtcc agcgacgcca gcataccgtc ccatttagtc ggttttagtg acgcctcaac
     3181 gccaagatgt tttgccagct gttgggcaaa ttccacttca aaaccggtta atttgccgtc
     3241 atccccctga aaactgaacg gcggataagt tccttccagc cctaccagca gcgtgccgcg
     3301 ctctttgact ttattaagca gaccttcatc tgcaaaactt ttaacgctca tgcccgcaac
     3361 cagcgccacg gccatcacac ccatcaatgc ctgacgtccc agatgtgcta atttcatatt
     3421 caccccgaat gttgttatgt ctgtttgcag tgtagagcca ttgtttgtaa acacaaaaac
     3481 aactccgcta catcttattc ttatttaata tatatcagaa gcaggttggc tggaggattt
     3541 ctgcacaagt ccggtacacg tttgacgctg atagtgttga tacttccgta acgcgatgcg
     3601 gtaattgttg gtgctggtgg agggcagcca cggggcgagg atttcatcaa ggaaaccgtc
     3661 ctgcagctga tcgagggaaa tattttgctc gtgtagatga ttccccaaac ggcgcagacg
     3721 aacgacatat tcacgcaccg taccagggct catttcagtt tgctcaaaaa gaaattgctt
     3781 aaagccgata atatcgaaaa aatcactctg cgttttgcaa tgtaaatcac cgcaaaatcg
     3841 gcacaatgcc gcccatgatt tttgttccgt ttgccagcca ttttcatcaa gcagcatgtc
     3901 caggcgggaa atctcgattt tattcactat tttgccgtcg cgaacgaggg taatgcggtc
     3961 gagtaattta cggcaatgcg cgcaatgggt ctggctgtgc ttaaagtctt taagatagcg
     4021 gcttaatggc cgtcttttca ggtgctgcac catcattaag aactcctggt agtcaaaatt
     4081 aaagtgcggc atttactgac gttataactt acccagttta gtgcgtaacc gtttaatagc
     4141 ctggctgtgt aactgactga cccgcgattc cccgacctcc agcaccgcgc caatctcttt
     4201 gagattcagc tcttcctggt aatagagggt taataccagt ttttcgcgct ctggcaacgt
     4261 ttcgatagct tccatcaccc gctggcgcag attactgtcc agcagttgtt gtagcgggtt
     4321 ttctcgctga tgatcatcag taaccagttc aatgctatcg ccgtgctctt cgcgccactc
     4381 atcgtaggag aagagctggc tgttattggt gtcgagcaac atttggcgat agtcggcaat
     4441 atcgatccct aaacgttccg ctacctcagt ttccgtggcg ttgcggccaa gttcctgctc
     4501 cagttgccct attgcctgtg ccacttcacg cgcgttgcgt cgcacgctgc gcggcaccca
     4561 gtcacggctg cgaagttcat ccagcatagc gccacggata cgctgcactg cgtaagttgt
     4621 aaatgccgtt ccttgtaggg cgtcatagcg ttcgacggca ttaagtaacc caatgccgcc
     4681 cgcctgtagc agatcgtcaa gttccacgct cgcgggcagt cgaacctgca ggcgcaatgc
     4741 ttcgtgacgc accagcggga cataacgctg ccacagcgag tgtttatcca ttacaccttc
     4801 agcggtatag agtgaattca cgataaacag ccctgcgtta tatgagttat cggcatgatt
     4861 atccgtttct gcagggtttt taatcggacg attagtgggt gaaatgaggg gttatttggg
     4921 ggttaccggt aaattgcggg cagaaaaaac cccgccgttg gcggggaagc acgttgctga
     4981 caaattacca ttcatgttgc cggatgcggc gtaaacgcct tatccggcct acaaaatccg
     5041 caaattcaat aaattgcaat tcaccttgta ggcctgataa gcgcagcgca tcaggcaatt
     5101 tggcgttgcc gtcagtctca gttaatcagg ttacgacgat tagccctgca gcagagacag
     5161 aacctgctgc ggtacctggt tagcttttgc cagcacggag ttaccggcct gctggatgat
     5221 ctgcgctttc gacatgttgg acacttcggt cgcatagtcg gcgtcctgaa tacgggactg
     5281 cgcttcggac aggttggtag tggtgttgtt caggttggtg accgcggaat ccagacggtt
     5341 ctggatagca cccaaggatg aacggaattt gtcgatggag ctgattgcgt cgtcaagcgc
     5401 agccaatgga tcggacgtct tcgatgtttt attctctgtg ctgtagacac catcagcgtc
     5461 tttatagatt tctttgcctg cgctattcgt gaccgtgcca tcggcttgcg agtagtaggt
     5521 tgaaacagtt tgagcatcct gggttaagtc atcccccgcc aggaaaccat ttgtagtatt
     5581 atttgcggca acagcataag tattcccacc ggtgttggcg gaatatgcaa tgcccgtaaa
     5641 tgcagatgct gaaacagtag caccttcaat ttttacagta cctgtattac cttcagctgt
     5701 aactgttaca ccagcctctg tcttgatggt tgttttaaca gcagtacctt tcgcatccgt
     5761 atttccgaga agcccagaaa cagttgcttt gttcgggttg ccggcctggt ttttagtcag
     5821 gttacctgtt gaatccagat aaagttgatc gccatcactt gaagtaatat tgcccgaatc
     5881 atcaatattt acattctgtg ctgtcccacc aatagtaact gatgcagctg tcgttttccc
     5941 tgctgcaggt atgagatttg ctgcagcatt cgtttggttt acaccgtcag ccgtatagga
     6001 gaaggtattg ctggttgcat tataagtata agttgtcgat gtcgcattat cagcaaggct
     6061 atttgcacct gttgcggtaa ttttatcgcc agtcttaagc gccgcaagaa tatcagatcc
     6121 ttttgctgca gtatttgctg tagttttagt atatgcagtg ctgccgtcag atgaagttaa
     6181 ccctttgata tatccgtctt taaccattcc gtcagctgtc gccgcgacac ttgccttatc
     6241 tgcgctacca tttacgttaa acccactcag ccccagcgta tctgagtcaa ttttctttag
     6301 atcaatagtg atagtctggc cgtcattcgc accaacctga attttcattg aaccgtcttt
     6361 cgccagtacg ttcacgccgt tgaactgagt ctggccagat acgcggtcaa tttcgtccag
     6421 acgagatttg atttcgtcct gaatggagtc cagatccgaa tcagagttag tcccggtaga
     6481 agcctgaacc gtcagctcac gaatacgctg taagttgttg ttgatttcgg acagcgcgcc
     6541 ttcggtggtc tgcgcaacag aaataccgtc gttggcgtta cgtgcagcct gagtcaggcc
     6601 tttaatatta gaagtaaaac ggttagcaat cgcctgacct gcggcgtcat ccttcgcgct
     6661 gttaatacgc aagccagaag acagacgctc gatagaactc gacagcgcag actggttctt
     6721 gttgatatta ttttgagtga tcagcgagag gctgttggta ttaatgactt gtgccatgat
     6781 tcgttatcct atattgcaag tcgttgatta cgtattgggt ttccacccgt cggctcaatc
     6841 gccgtcaacc ctgttatcgt ctgtcgtaaa acaaccttta gaattttttt cacaaacagc
     6901 cattttttgt tagtcgccga aatactcttt tctctgcccc ttattcccgc tattaaaaaa
     6961 aacaattaaa cgtaaacttt gcgcaattca ggccgataac cccggtattc gttttacgtg
     7021 tcgaaagata aaaggaaatc gcatggcaag tatttcatcg ctgggagtcg ggtcaggcct
     7081 ggatttaagt tcaatccttg atagcctcac cgccgcgcaa aaagcgacgc taacccctat
     7141 ttcaaatcag caatcgtcgt ttaccgctaa acttagcgcc tacggtacgc tgaaaagcgc
     7201 gctgacgact ttccagaccg ccaatactgc attgtctaaa gccgatcttt tttccgccac
     7261 cagcactacc agcagcacca ccgcgttcag tgccaccact gcgggtaacg ccatcgccgg
     7321 gaaatacacc atcagcgtca cccatctggc gcaggcgcaa accctgacca cgcgtaccac
     7381 cagagacgat acgaaaacgg cgatcgccaa cagcgacagc aaactcacca ttcaacaagg
     7441 cggcgacaaa gatccgatta ccattgatat cagcgcggct aactcatcgt taagcgggat
     7501 ccgtgatgct atcaacaacg caaaagcggg cgtaagcgca agcatcatta acgtgggtaa
     7561 cggtgaatat cgtctgtcag tcacatcaaa tgacaccggc cttgataatg cgatgacact
     7621 ctcggtcagc ggtgatgatg cgctacaaag ttttatgggc tatgacgcca gtgccagcag
     7681 caacggcatg gaggtctcgg tcgccgccca gaatgcgcag ctgacggtca acaacgtcgc
     7741 cattgagaac agcagcaaca ccatcagcga cgcgctggaa aacatcaccc tgaacctgaa
     7801 cgatgtcacc acgggcaacc agacgctaac catcactcag gacacctcca aagcgcaaac
     7861 ggcgattaaa gactgggtga atgcctataa ctcgctaata gataccttca gcagcctgac
     7921 caaatacacc gccgtagatg cgggagctga tagccagagt tctagcaatg gcgcactgct
     7981 cggcgactcc acgttgcgga cgattcagac gcagttgaaa tcgatgctga gtaataccgt
     8041 tagttcttcc aactataaaa cgttggcgca gattggtatc acgaccgatc ccagcgatgg
     8101 caaactggaa ctggatgccg acaaactcac cgctgcactg aaaaaagatg ccagcggcgt
     8161 aggtgcattg attgttggcg atagtaaaaa aaccggcatc acaaccacca tcggcagcaa
     8221 cctgaccagt tggctttcga caacgggcat tattaaagcc gctaccgatg gcgttagtaa
     8281 gaccctgaat aagttaacta aagactacaa cgccgccagc gatcgcattg atgcgcaggt
     8341 cgcgcgctat aaagagcaat ttacccaact ggacgtttta atgacctcgt taaacagcac
     8401 cagcagctac ttaacgcagc agttcgaaaa caacagtaat tccaagtaat caatattcat
     8461 cgggagacag gtcatgtacg cggcaaaagg cacccaggcc tatgcacaaa ttggcgtcga
     8521 aagcgccgta atgagcgcca gccagcagca gctggtcacc atgctatttg atggagtgct
     8581 gagcgcactg gttagagcgc gcctgtttat gcaggacaac aatcagcaag gcaaaggcgt
     8641 ctctttgtca aaagcgatca acatcattga gaacgggctg cgggtgagtc ttgatgaaga
     8701 gagcaaagac gaactaaccc aaaacttgat tgctctttat agctatatgg tcaggcgctt
     8761 gctgcaagcc aatttacgca acgatgtctc cgcagtcgaa gaagtggaag cattaatgcg
     8821 caatattgcc gatgcctgga aagagtcttt actctcccct tctttgattc aggacccagt
     8881 ctgatgaaca atgcaccgca tttgtatttc gcctggcaac aactcgtcga aaaaagccag
     8941 ctcatgttac gcctggcgac agaagaacaa tgggacgaac tcatcgccag cgaaatggcg
     9001 tatgtgaatg cggtgcagga gatcgcacac ctgactgaag aggttgcccc gtccaccacg
     9061 atgcaggagc agctccgccc gatgctgcgc ctgattctcg acaacgaaag caaggtaaag
     9121 cagttattac agattcggat ggatgaactg gcgaaactgg tcggtcagtc atcggtgcaa
     9181 aaatcggtgt taagtgccta tggcgatcag ggtggctttg tgctggctcc gcaggataac
     9241 ctcttttgaa tcttaaagag ccgatggctc gcgaataatc cgattacggc tacgcttcta
     9301 atattcccct tgaatggagt cgaagaatgc gtaatcccac gctgttacaa tgttttcact
     9361 ggtattaccc ggaaggcggt aagctctggc ctgaactggc cgagcgcgca gacggtttta
     9421 atgatattgg tatcaatatg gtctggttgc cgcccgccta taaaggcgca tcgggcggct
     9481 attcggtcgg ctacgactcc tatgatttat ttgatctagg tgagtttgat cagaaaggca
     9541 gcatccctac taaatatggt gataaagcac aactgctggc cgccattgac gccctgaaac
     9601 gtaatgacat tgcggtgctg ctggacgtgg tagtcaacca caaaatgggc gcggatgaaa
     9661 aagaagctat tcgcgtgcag cgtgtaaatg ctgatgaccg tacgcaaatt gacgaagaaa
     9721 tcattgagtg tgaaggctgg acgcgttaca ccttccccgc tcgtgccggg caatactcgc
     9781 agtttatctg ggatttcaaa tgttttagcg gtatcgacca tatcgaacac cctgatgaag
     9841 atggcatttt taaaattgtt aacgactata ccggagaagg ctggaacgat caggttgatg
     9901 atgaactcgg taatttcgat tatctgatgg gtgagaatat cgattttcgc aatcatgccg
     9961 tgacggaaga gattaaatac tgggcgcgct gggtgatgga acaaacgcaa tgcgacggct
    10021 ttcgtcttga tgcggtcaaa catattccgg catggtttta taaagagtgg atcgaacacg
    10081 tacaggaagt tgcgccaaag ccgctgttta ttgtggctga atactggtcg catgaagtcg
    10141 ataaactgca aacgtatatt gatcaggtgg aaggcaaaac catgctgttt gatgcgccgc
    10201 tgcagatgaa attccatgaa gcatcgcgca tgggtcgcga ctacgacatg acgcagatct
    10261 tcactgggac gctggtggaa gccgaccctt ttcacgccgt aaccctcgtt gccaaccacg
    10321 acacccaacc gttgcaagcc ctcgaagccc ccgtcgaacc gtggtttaaa ccgctggcgt
    10381 atgccttaat tttgttgcgg gaaaatggcg ttccttcggt gttctatccg gacctctacg
    10441 gtgcacatta cgaagatgtc ggtggtgacg ggcaaaccta tccgatagat atgccaataa
    10501 tcgaacagct tgatgagtta attctcgccc gtcagcgttt cgcccacggt gtacagacgt
    10561 tatttttcga ccatccgaac tgcattgcct ttagccgcag tggcaccgac gaatatcccg
    10621 gctgcgtggt ggtcatgtcg aacggagatg atggcgaaaa aaccattcat ctgggagaga
    10681 attacggcaa taaaacctgg cgtgattttc tggggaaccg gcaagagagc gtagtgacag
    10741 acgaaaacgg cgaagcgacc ttcttttgca acggcggcag cgttagcgtg tgggttatcg
    10801 aagaggtgat ttgaatccat ccccggcggc aagccgggga gatttcatta cggcagtggc
    10861 gtcggtaacg gcgctttatc cagcgcggcg gcacactctg ccgtcgggcg atccacacgc
    10921 tccatcgtca taccatcgta ctcaatggtg ttaccgtcac gctcaacctc gtacagctca
    10981 cgttttacgg taacgttagt gagatcatca aacatcaatg tcaatttacc cggcacggca
    11041 atcacccgct gccactgacg gcaatcgagg gtatcgccct ctttagtgac aatcaggctg
    11101 cctatcgctt ccggactgac cagcgcacgt tgcggccctt tggtctgcca gtagcccgcc
    11161 agccagtccg gcgcgggggt tttcacgaca ttgttatagt tttcgacttc ggcacagccc
    11221 gccagcaaca tgagcgcacc tgcaattgcg agttttttca tcattcgtcc tgcatgagaa
    11281 gaaaagagga ttgtggcatt aaagccctga tgtcgccagc ctttccaggt tcagagagaa
    11341 atttgatcta accattattt ttgtgaatta ttaccccgaa aataaatttg catggtgtat
    11401 gattcgcgac tcttgtttca aaccttctga gttcagaggc tacattcatg tcatggcagc
    11461 aattcaaaca cgcctggttg attaaattct gggcgcccat ccccgcggtc atcgcggcgg
    11521 gtattctctc cacttactat tttggcatca ctggcacctt ttgggctgtc acaggtgaat
    11581 ttacccgttg gggcggtcag ctcctgcagt tgttcggcgt ccatgctgaa gagtgggggt
    11641 attttaaaat tatccatctg gaaggatcgc cattaacccg catcgacgga atgatgatcc
    11701 tcggtatgtt tggcggctgt tttgccgcag cgctgtgggc caacaatgtc aaactgcgca
    11761 tgccgcgcag ccgtatccgc attatgcagg ccatcattgg cggtattatc gccggttttg
    11821 gcgcacgtct ggcaatgggc tgtaacctgg cggcgttctt taccggaatt ccacagttct
    11881 ctctgcacgc ctggttcttt gccatcgcca ctgccattgg ttcatggttt ggcgcgcgct
    11941 ttaccctgct gcccatcttc cgtattcccg tgaaaatgca gaaagtttct gctgcctcac
    12001 cgctgacgca aaaaccggat caggcgcggc gtcgttttcg tctcgggatg ctggtctttt
    12061 tcggcatgct gggctgggcg ctgctgacgg cgatgaacca gcccaaactg gggctggcaa
    12121 tgctgtttgg cgtcggcttt ggtttgctga ttgaacgtgc gcaaatctgc tttacttcgg
    12181 cgttccgcga tatgtggatc accggacgta cccatatggc gaaagcaatc attatcggta
    12241 tggcggtaag tgccatcggg atcttcagtt acgtacagtt aggcgttgaa cccaaaatca
    12301 tgtgggcggg accaaacgcg gtaattggtg gtttactgtt tggttttggc atcgtgctgg
    12361 caggcggctg cgaaaccggc tggatgtacc gcgcggtaga aggccaggtg cactactggt
    12421 gggtcggtct gggcaacgtg atcggctcaa cgattctggc gtattactgg gatgatttcg
    12481 ctccggcgct ggccaccgac tgggacaaaa tcaacctgct gaaaaccttt ggcccgatgg
    12541 gcggcctgct ggtgacatat ttgctgctgt ttgctgcgct gatgttgatt atcggctggg
    12601 aaaaacgctt cttccgccgt gcggcaccgc agactgctaa ggagatcgca tgaaaaatat
    12661 cgttcctgat taccgccttg atatggtggg tgaaccctgc ccttatccgg cagtcgccac
    12721 ccttgaggcg atgccgcaat taaaaaaagg tgagatcctg gaagtggtga gcgactgtcc
    12781 gcagtcgatc aataatattc cactggatgc gcgtaatcac ggctatacgg tgctggatat
    12841 tcagcaagac gggccgacca ttcgttattt aattcagaag taatccctca ttcccgctgt
    12901 ggcccacagc gggaacatcg ttgcctctga cacatagatt ttacccgccc cctctcctat
    12961 tcttttcagt atgatctcag gaggttgcta tgtgtggacg ctttgcccaa tcccaaacgc
    13021 gtgaagatta ccttgcgctt ctcgcggaag atattgaacg cgatattccc tacgatccag
    13081 aacccattgg cagatacaac gtcgcgccgg gaaccaaagt tcttctgctc agtgaacgtg
    13141 atgaacacct tcatctggat ccggttttct ggggatatgc tcccggatgg tgggataaac
    13201 cgccgctgat taacgcccgc gtagaaactg ccgcaaccag tcgcatgttt aaaccgctct
    13261 ggcaacacgg tcgggcaatc tgttttgccg atggctggtt tgagtggaaa aaagaaggcg
    13321 acaaaaaaca gccttatttt atctatcgcg ctgacggaca acctgttttt atggccgcga
    13381 taggtagcac accgtttgag cgtggtgacg aagccgaagg atttttgatc gtcactgcgg
    13441 cggcagatca aggtctggta gatattcata accgccgccc gctggtactg tcgccagaag
    13501 ctgcgcggga atggatgcgg caagaggttg gcggtaaaga agcctcagaa atcgcggcca
    13561 gtggctgtgt tacagcaaac cagttcacct ggcacccggt atcgtgcgcg gtgggtaatg
    13621 ttaaaaacca gggcgcggag ttaattcaac ctgtttgata agcctggaga ttatctacac
    13681 ctggatggac atcattgtac tatgccccgt acatgcccca tcacatcctc aacatcaaag
    13741 tattagagcc ttgcaaacca ttatgcgtct cagtttgtct catctatctt acgcaaaaaa
    13801 aaatagaact aatatcaagc ggcgctatat tgcgtattaa ataaaaccac
//
LOCUS       DAZFJL010000123        13473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZFJL010000123 DAZFJL010000000
VERSION     DAZFJL010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.125"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..609
                     /locus_tag="ACHJ8C_003644"
     CDS             <1..609
                     /locus_tag="ACHJ8C_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFO5553432.1"
                     /translation="KGILKENSFMMSKIVNKKYEKGLSLIESAMVLALAATVTAGVMF
                     YYQSASDSNKTQNAISEVMSATSAINGLYIGQTSYNGLNAQILLNSSAIPDNYKGDDH
                     ISNPFGGKLIPAPASTSNSSFGYYVQLDGLDKSACVSLATLNLGTSAKGYGINISSPA
                     DTTAFQSTASSGQAKNSAITPAEAANSCTKENSDNTVTYFMK"
     gene            656..1057
                     /locus_tag="ACHJ8C_003645"
     CDS             656..1057
                     /locus_tag="ACHJ8C_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin co-regulated pilus biosynthesis Q family
                     protein"
                     /protein_id="HFO5553433.1"
                     /translation="MRILIFFICLLVSNITHAQESTNKGSRLFSGNATSVAPQSSERT
                     VKDDKTDWRVIKGQTLYGVLSDWGQKSGWTVLWNTDYNYTLNASAVFKDMSLDDAVKK
                     LLESMGAVSPKVFIKFYNGNHVIVVTQSPEG"
     gene            1064..2722
                     /gene="bfpB"
                     /locus_tag="ACHJ8C_003646"
     CDS             1064..2722
                     /gene="bfpB"
                     /locus_tag="ACHJ8C_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bundle-forming pilus secretin BfpB"
                     /protein_id="HFO5553434.1"
                     /translation="MKLGRFSLFLLCPLLASCSGNGFYKDNQRVIDKNILHTDTSLLK
                     SKNKKYYKSSDMVSKTDSIYIGNSSFQTYHGEPLPGKLEGVHGIVLRSSAPLEFDEVL
                     SMIQDASGIPVVKQTTKDVISGGVSSKSLAAAVAEKMNRAAGVNSTDHFDNLMLGVSS
                     NHQIMDVNYQGALSSFLDKVAANYNLYWTYESGRIVFSNEETKRFSISILPGGKYTSK
                     NSISSDSNSSSGSSGSSGSSSSDSGAELKFDSDVDFWKDIENSIKLILGSDGSYSIST
                     STSSVIVRTSSANMKKINEYINTLNAQLERQVTIDVAIYNVTTTDSSNLALSLEALLQ
                     RNGGVLGSVSTSNFAATSGTPSLTGYLNGNGDSSNQVLLNLLAEKGKVSVVTSASVTT
                     MSGQPVPLKVGNDRTYISEIGTVLSQSSTSTTATTSTVTSGFLMNLLPQVADDGNILL
                     QYGVTLSELVGSNDGFDQATVNGTVIQLPNVDSTTFVQSSMLRNGNTLVLAGYEKKRN
                     ESVDQGVGTTSFKLLGGALSGSASRTVTVICITPRIIDLKASGE"
     gene            2719..3927
                     /locus_tag="ACHJ8C_003647"
     CDS             2719..3927
                     /locus_tag="ACHJ8C_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553435.1"
                     /translation="MIKDNLGVAVIGSKQYAVNLLWGSSQDTETTNQALNKSLALMSS
                     KLYSVIERFQGEQFAVGDKNIGHKRGLVTLLSAIDFDGSSFCGLFPTDNELWLVIGVD
                     KDGMVHFDKSFYSKDDAKKFFFDHVAYRYPWDRTYSPSDVGVGESKSITELSLIKGKK
                     LKEKGAGQLMPVLFFGAGIIICFVIIYNAIDSWLSGKDKDVINEVSPPVISEEVRNVP
                     WGGKSKPASLFSQCIKEMDSFRFLAASVPGWMPEDKVTCKDSNISFSAHRSGGLDIWY
                     EDPSLFFHSGNIPGITKNGNDIITLTWPLDTVRYDSLRLDVNRLNNTQEIIRYLSRQF
                     ENSWINIKFGQPVPDGNTERVDFSFSLNNDPRILLPILSEVNGLVITNIDYDFSGSNW
                     NVSGVFWGGA"
     gene            3929..4399
                     /locus_tag="ACHJ8C_003648"
     CDS             3929..4399
                     /locus_tag="ACHJ8C_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001815797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553436.1"
                     /translation="MKKILFTLLLVVSSVVNAEVEKSVDVSGENDIRKEEKKLPDVDL
                     EQYKDELLSYQQLQRKNMMLKLQAENQKLESEIKKNGSFVSYDTDDNTMHVMSIMTIP
                     KRGLCATIFYGGIRRVCKGDVINEDYVVSDITGNYVEIRMVNDISDTGRKIYLR"
     gene            4406..6013
                     /locus_tag="ACHJ8C_003649"
     CDS             4406..6013
                     /locus_tag="ACHJ8C_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFO5553437.1"
                     /translation="MMLNNTENNSGLTFERFKRNVSEIITGEGGELELTDEQRKYFLI
                     FRNGDFLVSSCHINHHLVQMLREIAARKGYPNLTIYEVNLKDIRLLYEASLRTVQKKT
                     QDLLPVEKRASTLLFECAEMRVSDLHIKVYDTEADIYIRKDGDMELLRQIESHTAHSI
                     LASLYNNADDADATYKINAYQAARIVASKSRLALPPVVQAVRLQFNPLGQGGRYLIAR
                     FLYTDKSEQRKEIDPTRFGFHRLHAESFSRMRALPIGINIISGPTGSGKSTTLKNLLE
                     LLYIEKKKKVNIISIEDPPEYEIEGTAQLPITNVETEAQRGEEYRKAITAALRSDPDI
                     IMPGEARDAEVINLLFTAAMTGHQVWTSLHANNALAIFDRLKDQGVDEFKLTDPELLT
                     GLVAQRLVRRLCDQCSITLTEYTDNGGDLSARDRKIISGHERTVRFPNPRPEKCCGDG
                     YNGRTIIAEVIEPDSKLLRLVSEGKREEAQHYWLTSLGGMALKEHAWLKIVSGEICVM
                     DAVNKTSGIDNISEERKKYLLSRNNEI"
     gene            6020..7078
                     /gene="bfpE"
                     /locus_tag="ACHJ8C_003650"
     CDS             6020..7078
                     /gene="bfpE"
                     /locus_tag="ACHJ8C_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bundle-forming pilus protein BfpE"
                     /protein_id="HFO5553438.1"
                     /translation="MKEKLNRLLFTSKTRMRVFSKLSRYLSNGVPVTFALAELYKFTS
                     DEGRKKDNPDAFALQRWLIAVRNGKTLAEAMEGWVPSDELSIISAGEISGNVHHALDD
                     IIYMNDTKKKVKGALAGIIYPVVLLLTTCLYLHIFGTQVVPAFSGILPVEKWQGAGRT
                     MYYLAVFVQNYLAITLLSVMMVMLLILATLSRWTGRLRLFFDRFIPWSIYKTIIGCGF
                     LLSLASLINAGIPVPEALRIIMKTASPWYKEKLVAIRYRMLNGDRNLGEALNSSGYIF
                     PSKQMIMDLRSYSALGGFDEMLNKLSVQWQDDSVAYITKQMSVLKNVAIIIMGLVFMW
                     IVSGMFSLQQQISDAARF"
     gene            7097..8053
                     /gene="bfpF"
                     /locus_tag="ACHJ8C_003651"
     CDS             7097..8053
                     /gene="bfpF"
                     /locus_tag="ACHJ8C_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bundle-forming pilus retraction ATPase"
                     /protein_id="HFO5553439.1"
                     /translation="MNIINELNFADLLISKDSYNFRFLEGQPYPICTVPANYNAEVQG
                     MMQELESIREVKGNEFFYLHLGVPYRVAVVQTINGSGFFLRKLKLPVPSLDSLGFSEP
                     FLNTLKLMGKKRGLLLVSGATGSGKSTTIYALLTYYVQKYGDIAISIEDPPEVPVQGN
                     YGNNQEGVWFQMDARELGGYENAMISAMRYNPRYIFLGEIRSAKTAKEAIRAAVNGHL
                     VVSTIHGSSVQGAIYALQQIASAEGEADLVRSIIADGLLGIVHQELRFTERGERSLSA
                     NILCNDGSASISSKIRSGKLELLSSEIEQQRIMLSHGKTLIT"
     gene            8060..8809
                     /locus_tag="ACHJ8C_003652"
     CDS             8060..8809
                     /locus_tag="ACHJ8C_003652"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO5553440.1"
                     /translation="MQESILLILVFIYATTIASFIWLAVERLPHQLKWVDDPVSDLTI
                     WSPGSKCNHCGEKIRWYYLIPVLGYLLCRGKCVYCLSKVPSRYPLTELFCGICCVIIF
                     IFFRDRMYDAVIVSLLFLCLVFLTLIDLRENWLPACVTYPLFWAGMIIPGFASSDDKI
                     IGAFAGFFIMYVSMKLVSAVRKEDVFAGGDVALATAAGAWLGIDKMPLFLILSSVIFI
                     FYSLPARFRGQVFVPMGPALSVSFFICLVYH"
     gene            8791..9237
                     /locus_tag="ACHJ8C_003653"
     CDS             8791..9237
                     /locus_tag="ACHJ8C_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO5553441.1"
                     /translation="MFSLPLITGIAACVINASSVNNIDPELLLSVMTVEGGTPGSVSV
                     NKNGSQDLGIMQINTHAWLKPISKSFFDGDESSAYNKLKDDACFNISVGAWILRHSIK
                     LEGGNVWEGVGRYHSATPEYKYRYIDKVKNVYGKMTYSQSTHKRNM"
     gene            9257..9796
                     /locus_tag="ACHJ8C_003654"
     CDS             9257..9796
                     /locus_tag="ACHJ8C_003654"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553442.1"
                     /translation="MFILGNKRKESGLSLIEASLVIILAGLVIVLIQSAISKIIKTAV
                     HENALHDISYVMSNIQDTLQTSCYEKSDDVCEKKITSVTLKFNTKKIKVDIKKTGKYV
                     AVYVLYTLSDNEDFGRYEDFGSMLLTHASAYYGRIDGKGAVNSVLGWWTVNSNSTIFS
                     GGDNNKNLFFYNNFRVNTL"
     gene            9793..10341
                     /locus_tag="ACHJ8C_003655"
     CDS             9793..10341
                     /locus_tag="ACHJ8C_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001815798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein J"
                     /protein_id="HFO5553443.1"
                     /translation="MIGKEKGLSLIEVMIGILFFSVISLSVYDHYNRSAEQHYINNIS
                     IVMCRYADALSYYLQEDDKVNNSSVSLSELVSSGYLPSMPEGASVGGYKIKMYVDNKD
                     NGLIALYGDEHYSLTIKSMKPFLGIRGASVEGDKIISTGGYYSLPVSGFTNFSTDNLR
                     GLMLIPSIQTSGQNCDRGGYDF"
     gene            10316..10819
                     /locus_tag="ACHJ8C_003656"
     CDS             10316..10819
                     /locus_tag="ACHJ8C_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553444.1"
                     /translation="MTEVDMIFEGRQQKGQSLIEIAMVIILSVLFISGGSYIMTLFKT
                     FSQSCVVATDMNSIRSIIASSTVKYKDKKYDASIITNNNRFNKYQVYFGDDGYTLISG
                     GSPVEIDEFYITCYLAKYNCVWIINQNNNLYAKHMSAVLSPELLSTYGISYSGDSGVT
                     GLLFKRY"
     gene            10822..11271
                     /locus_tag="ACHJ8C_003657"
     CDS             10822..11271
                     /locus_tag="ACHJ8C_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553445.1"
                     /translation="MLLFWCGFFSSIALILSLSQGYNERLYLDEQDRITKENIYVDYL
                     SVNADVLFDICATEQNRCNNIHTTLPEEFAAQLKEKLSNYDNSITPGFCINNGEIIVY
                     TSHNFNIERYKDSSLFNLSDVQSDIKTACSIHDYSGIAVIKMLNKNK"
     gene            11737..11865
                     /locus_tag="ACHJ8C_003658"
                     /pseudo
     CDS             11737..11865
                     /locus_tag="ACHJ8C_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11965..13193
                     /locus_tag="ACHJ8C_003659"
     CDS             join(11965..12280,12280..13193)
                     /locus_tag="ACHJ8C_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HFO5553446.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSPVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAVMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSLVSRCLRMSRAQLHVILRRTDDWMDGRRSRHTDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLGRKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     PESNGIAESFVKTIKRDNISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQRACNGLSDNRCLEI"
BASE COUNT         4043 a         2308 c         3089 g         4033 t
ORIGIN      
        1 aaggggattc ttaaggaaaa cagttttatg atgtctaaaa tcgtgaataa aaaatatgaa
       61 aaaggtctgt ctttgattga atcggcaatg gtacttgcac ttgctgcaac cgttaccgct
      121 ggtgtgatgt tttactatca gtctgcatct gattccaata aaacacagaa tgctatttcc
      181 gaagtcatga gtgctacttc tgcaattaat ggtttgtata ttggacagac atcttataat
      241 ggattaaacg cacaaatttt acttaattcg tctgcaattc cagataacta caagggagat
      301 gatcatattt ctaacccgtt tggaggtaaa cttattccgg ctccagctag cacaagtaat
      361 agctcatttg gttattatgt acaactcgat ggattggata aatctgcatg tgtcagtctg
      421 gcaactctaa acttaggaac atcggctaaa ggctacggta taaatatttc ttccccagct
      481 gacacgactg ctttccagtc gacggcttca agcggccagg caaaaaacag tgctattact
      541 cctgcagaag cagcgaactc ctgtaccaag gagaatagtg ataatacagt tacatatttc
      601 atgaagtaat ttacacagca cgcactgtat tgtgcgtgct tcatgggagg tgaatgtgag
      661 gattctaatc ttttttattt gtcttttggt atccaatatc actcatgctc aggaaagtac
      721 aaataaaggc agcaggcttt tttcaggaaa tgcaacgtca gtcgcaccac agtcaagtga
      781 aagaacagtt aaggatgata agactgactg gcgtgtaata aaaggacaga ctttatatgg
      841 cgtgctttct gactgggggc aaaagagtgg atggacagtg ctatggaata ccgattataa
      901 ctatacttta aatgcatctg cagtctttaa ggatatgtcg ctggatgatg cggtaaagaa
      961 attattagaa tcgatgggag ccgtgtctcc aaaggtcttt attaaatttt ataacgggaa
     1021 tcatgtcatt gtcgtgacac aatcgcccga aggataatta aaaatgaaac taggcaggtt
     1081 ttcacttttc ttattgtgtc cgcttctggc atcttgctcg ggtaatggtt tttataaaga
     1141 caatcagagg gtaatcgata aaaacatttt gcatactgac acctcattgc tgaagtcgaa
     1201 gaataaaaaa tattataaaa gcagtgatat ggtaagcaaa acggacagta tttatattgg
     1261 aaacagtagt ttccagacat atcatggtga acccctgcca ggcaaattag aaggtgtaca
     1321 cggaattgtt ttgaggtcaa gtgctcctct ggaattcgat gaagtgctct ctatgattca
     1381 ggatgccagt ggtattcctg ttgtaaaaca gaccacgaaa gatgtgatca gtggaggggt
     1441 gtccagcaaa agtctggcgg ctgctgttgc ggaaaaaatg aaccgtgccg cgggggtgaa
     1501 tagtactgac cattttgata atctgatgct tggggtatca tcaaaccatc agattatgga
     1561 tgttaattat cagggtgctc tttcttcgtt tcttgataaa gttgcggcaa actataacct
     1621 gtactggaca tatgagtctg gcaggatcgt tttttcaaac gaggaaacta aacgattcag
     1681 tataagtata ttaccaggtg gtaaatatac atcaaaaaac tctatcagtt cggacagcaa
     1741 tagcagttcg ggaagttcgg gaagttcggg aagtagttcc agtgattccg gggcagagtt
     1801 gaaattcgac tcagatgtgg atttctggaa agatattgag aattcgataa agttgatatt
     1861 aggtagcgac ggctcatatt caatatcgac aagtacatcg tctgttattg tcaggacatc
     1921 ttctgcaaac atgaagaaga taaacgagta cattaataca ctgaacgcac aacttgaaag
     1981 acaggttacc attgatgttg cgatctataa tgttacaaca actgacagca gtaatctggc
     2041 attgtctctg gaagctttgc tacagcgtaa cggtggtgtt ctgggctctg tttcgacctc
     2101 caattttgcg gcaacaagcg gaacaccttc actaacggga tatctgaatg gaaatggcga
     2161 ctcaagtaac caggttttac ttaacttact agccgaaaaa ggaaaagtca gcgtggtgac
     2221 atcggcatca gtaacgacga tgtcaggtca gccggtgcct ttgaaagtag gaaatgacag
     2281 aacatatata tctgagatag ggacggtgtt aagccagtca tcaaccagta caacagccac
     2341 tacatcaact gtcaccagcg gttttctgat gaatttgcta ccgcaggttg ctgatgacgg
     2401 aaatatactg ttgcagtatg gtgtaacact ttcagaacta gttggatcaa atgatggttt
     2461 cgatcaggca acagtgaatg gaacagtgat tcagttacct aatgttgatt ctacaacctt
     2521 tgtgcagtcg agcatgcttc gcaatggtaa tactcttgtt ctggcaggat atgagaagaa
     2581 aagaaacgaa agtgtggatc agggtgttgg gacaaccagt tttaaactct taggtggcgc
     2641 actgagtgga agtgcatccc gaacagttac tgtcatttgt ataaccccga gaattattga
     2701 tctcaaggct tctggcgaat gataaaggat aatctgggcg tagcggtaat tggttcgaaa
     2761 caatatgccg ttaatttatt gtggggaagc tctcaggata cagaaacaac aaaccaggct
     2821 ctgaataaga gcctggcgtt gatgtcctca aagctatatt ccgttattga acgatttcag
     2881 ggtgaacagt ttgctgtagg agataaaaat attggtcaca aaagaggcct ggtcacccta
     2941 ctgtctgcaa ttgattttga tggtagttct ttttgtgggt tatttcctac cgataatgag
     3001 ttgtggcttg ttattggtgt agataaagat ggaatggttc actttgacaa gtcattttac
     3061 agtaaggatg atgctaaaaa attttttttc gatcatgtag catatagata cccgtgggac
     3121 aggacataca gcccttcaga tgttggagta ggtgaaagca agagtattac cgagctttcc
     3181 cttattaaag ggaaaaaatt aaaggaaaaa ggtgctggtc aactaatgcc agtgcttttc
     3241 tttggcgctg gaattataat atgcttcgtc atcatatata atgctataga ctcatggtta
     3301 agtggtaaag ataaggatgt tattaacgaa gtgtcacctc cagtaatcag tgaggaagtc
     3361 aggaatgtcc cgtggggagg gaaaagcaaa ccagcttcat tgttttcaca atgtataaaa
     3421 gaaatggatt cattccgttt cctcgcagca tctgttcctg gttggatgcc tgaagataaa
     3481 gtgacctgta aggactctaa tataagcttc agtgctcatc gttctggcgg gctggatata
     3541 tggtatgaag atccatcctt gttttttcac tcgggaaata taccaggtat cacaaaaaat
     3601 ggtaatgata taataacatt gacatggcct ctggatacgg ttagatatga cagtttgaga
     3661 cttgacgtaa acagattgaa taatactcag gaaataataa gatacctttc acgtcagttt
     3721 gagaatagct ggattaatat aaaatttgga cagcctgtac cagacggtaa cacagaacga
     3781 gtggattttt ctttttcatt aaacaatgac ccaagaatct tgcttccaat tctttctgaa
     3841 gtaaatggac tggttattac taatattgat tatgattttt caggaagtaa ctggaatgtg
     3901 agtggtgttt tttggggggg ggcttagttt gaaaaaaata ttgtttactc ttttgcttgt
     3961 tgtttcttca gtcgtgaatg ctgaggtaga gaagagtgtt gatgtttctg gtgagaatga
     4021 tatcagaaag gaagaaaaaa aattgcctga tgttgatttg gaacagtata aagatgagtt
     4081 gctttcttac cagcagcttc agcgtaagaa tatgatgctt aaattacagg ctgaaaatca
     4141 aaagctggag agtgaaataa aaaaaaatgg atcatttgtc tcatatgata cagatgataa
     4201 caccatgcat gtaatgtcta taatgacaat tcctaaaaga ggattatgtg caaccatatt
     4261 ttatgggggc atacgcagag tctgtaaagg tgatgttatt aatgaagatt atgtggtgag
     4321 tgacattact gggaattacg ttgagatcag gatggttaat gacatatctg acaccggaag
     4381 gaaaatttat ttgaggtagg ttagtttgat gttaaacaac acagaaaata attcaggcct
     4441 gacctttgaa cgatttaagc gtaatgtatc tgaaatcatc acgggagaag ggggggagct
     4501 tgagcttact gatgaacaga gaaaatactt tctgatattc aggaatggtg attttcttgt
     4561 ctcttcatgc cacattaatc atcatctggt tcagatgtta cgtgagattg cagcaagaaa
     4621 agggtatcca aatctcacaa tttatgaggt gaacttaaaa gatatcagat tattgtatga
     4681 agcatctctc agaaccgtac aaaaaaagac acaggattta ttgccagtag agaagcgtgc
     4741 ttcaacgcta ctttttgaat gtgccgagat gagagtaagt gatttacata ttaaagtgta
     4801 tgatactgag gctgatattt atatacgtaa agatggagat atggaactct tgcgtcagat
     4861 tgagtctcat acggcgcatt ctatccttgc atcattatat aataacgcag atgatgcaga
     4921 tgcaacctat aaaattaatg cttaccaggc tgctcgcata gttgcttcaa aatcgagact
     4981 agcgttacct cctgtagttc aggctgttcg cctgcagttt aaccctcttg gtcagggtgg
     5041 gcgatatcta atcgcacgat tcttgtatac ggataaaagt gaacaacgaa aagaaataga
     5101 tccaaccaga ttcgggtttc atcgtttaca tgctgaatca ttttcaagaa tgagggcact
     5161 cccaattggt ataaatatta tctccgggcc gactggttcg ggtaaatcaa caacccttaa
     5221 aaatttactt gagttgttgt atattgaaaa aaagaaaaaa gtcaacataa tcagtattga
     5281 agatcctcct gagtatgaaa ttgaaggtac ggctcagtta cctataacaa acgttgaaac
     5341 agaggcgcaa aggggcgaag agtatcgtaa agccattaca gccgctttgc gttcagatcc
     5401 ggatataata atgccagggg aggccaggga tgctgaagta ataaatcttc ttttcactgc
     5461 agcaatgaca gggcatcagg tgtggacatc attacatgca aataatgcac tggccatatt
     5521 tgatcgatta aaagatcagg gtgtggatga atttaaactt acagatcctg aattgctcac
     5581 tggattggtt gcccagcgat tagtccggag attatgcgac cagtgtagta ttacattaac
     5641 agaatataca gacaatggtg gagatctatc tgccagggac agaaaaataa tttcagggca
     5701 tgaaagaact gtacgattcc ccaatcctcg acctgaaaaa tgttgtggag atggttataa
     5761 tggaaggaca atcatcgctg aagttattga acctgactca aaattactga ggttggtttc
     5821 tgaaggaaag cgtgaggaag ctcaacacta ttggttaaca tctcttggtg gtatggcctt
     5881 aaaagaacat gcatggttaa aaatagtttc tggtgaaata tgtgttatgg atgcagtaaa
     5941 taaaacttca ggaatcgaca atatttcaga agagagaaaa aaatatcttc tcagcagaaa
     6001 taatgagata tagttttcta tgaaagagaa attaaacagg ttgctattta cgtcgaaaac
     6061 gagaatgcgt gtttttagca aattatccag atatctaagc aatggtgtcc cagtaacgtt
     6121 tgctctggcg gagctttata agtttacgtc tgatgaggga agaaaaaaag ataatccgga
     6181 cgcttttgct ctacagagat ggttaattgc tgtaagaaat gggaaaacat tagctgaagc
     6241 gatggagggg tgggttcctt ctgatgaact gtcaataatt tccgccgggg aaatctcagg
     6301 taatgttcac catgcgttgg atgatatcat ttatatgaat gatacaaaaa agaaagtaaa
     6361 gggtgcactg gcaggaatta tttatccagt agtcctgctt ctgacaacgt gtctgtattt
     6421 gcatatcttt gggactcagg ttgttccggc tttttcaggc atcctgcccg tagagaaatg
     6481 gcagggagca ggcaggacta tgtactatct tgctgtattc gttcaaaatt atcttgctat
     6541 tacattgttg tctgttatga tggttatgtt attaatactg gcaacactat caagatggac
     6601 tggacgattg cgtctcttct ttgacagatt tattccctgg tcgatataca aaaccattat
     6661 aggatgtggt ttcctgttat cactagcatc gcttataaat gcaggtattc ctgtaccgga
     6721 agccttacga ataataatga aaacagcaag tccttggtat aaggaaaaat tagttgctat
     6781 aagatacagg atgttaaatg gagatagaaa tcttggtgaa gcccttaatt catctggtta
     6841 tatttttcca tcaaagcaga tgataatgga cctccgttct tactctgcgc ttggcggttt
     6901 tgatgaaatg cttaataaac tttcagttca atggcaggat gacagtgttg cctatataac
     6961 gaaacaaatg tctgttttaa aaaatgttgc tatcattatt atggggcttg tttttatgtg
     7021 gatagtatca ggtatgtttt cattacaaca acaaattagc gatgcggcaa gattctgatt
     7081 tttttggtgt agcataatga acattattaa tgaactcaac tttgcggatt tactaatctc
     7141 aaaggattca tataacttcc gttttctcga agggcaacca tatccaatat gcactgttcc
     7201 tgctaattat aatgctgaag ttcagggaat gatgcaagag ctcgagtcta tccgggaagt
     7261 aaagggaaat gagttttttt atcttcatct tggtgtgcct tatcgcgttg cggtcgtgca
     7321 aacaattaac ggttctggtt ttttcttacg aaagctgaaa ctcccggtac catcacttga
     7381 tagcctcgga ttttccgagc cattccttaa tacattaaaa ttgatgggga agaagagagg
     7441 attgcttttg gttagtggcg ctacaggctc aggtaagtca acaactattt atgctttgct
     7501 gacatattat gtccaaaagt acggtgatat tgctatttca attgaggatc caccggaagt
     7561 tcctgttcag ggaaactatg gtaataatca ggaaggcgtc tggtttcaga tggatgcacg
     7621 tgagcttggg ggatacgaaa atgccatgat ttcagcgatg cgttataatc caagatatat
     7681 tttcctgggg gagattcgta gcgccaaaac agcgaaagag gccattcggg ctgcagttaa
     7741 tggacatctt gtcgtttcaa caatacacgg aagctccgtt cagggggcga tttatgcatt
     7801 acaacagata gcttctgctg agggagaagc agatcttgtt cgtagtatca ttgctgatgg
     7861 attactgggg atagtccatc aggagttacg ttttacagaa agaggagagc ggtcactcag
     7921 cgcaaacatt ttatgtaatg atggctctgc atctatttct tcaaaaataa gaagtggaaa
     7981 actggaactc ctgagtagtg agattgagca acaacgaatt atgttgagcc atgggaaaac
     8041 actgattacc taaaatatta tgcaagagag tatattacta atattagttt ttatatatgc
     8101 gaccacaatt gccagtttta tttggctcgc ggtggagcgt ctgccacatc agttaaaatg
     8161 ggtggacgat cctgtttctg atttgacaat atggtcacca ggaagtaaat gtaatcattg
     8221 cggtgagaaa attcgatggt attatcttat tcctgttctc ggctatttat tgtgccgcgg
     8281 gaaatgtgtt tattgtctct ctaaagttcc cagccgatac ccattaacag agcttttctg
     8341 tggcatctgc tgtgttatta tatttatatt ttttagagac aggatgtatg atgcggtaat
     8401 cgtgtcgctt ctttttcttt gccttgtttt tcttactctg attgatttaa gagagaactg
     8461 gttaccggct tgtgttacgt atcctctgtt ttgggctggt atgattatcc ctggatttgc
     8521 atccagtgat gacaagatta tcggtgcatt tgccggtttt tttataatgt atgtctctat
     8581 gaagttggtt agtgcggtaa gaaaagagga cgtgtttgcg ggcggggatg ttgctcttgc
     8641 tacagctgcc ggagcatggt taggaataga taaaatgcct ttatttctca ttttgtcttc
     8701 agttatattt attttttact cccttcctgc aagattcaga ggtcaggttt ttgttcctat
     8761 gggaccagct ctttcagtat ctttttttat atgtttagtt taccattaat tacaggtata
     8821 gcagcatgtg ttatcaatgc cagttctgtg aataatattg atcctgaatt attactgtca
     8881 gttatgaccg ttgaaggcgg tactccaggt agtgtttccg ttaataagaa tggaagtcag
     8941 gatttaggta ttatgcagat aaatactcat gcatggttaa aacctatcag taagagtttt
     9001 tttgacggag atgagtcgtc tgcttataat aaacttaagg atgatgcctg ttttaatata
     9061 tcagtaggag cctggatcct tcgccattct attaaacttg agggtggtaa tgtatgggaa
     9121 ggtgtaggcc gatatcattc ggccacacca gagtataaat acagatatat agataaagtg
     9181 aaaaatgtct atggaaaaat gacctatagt caatcaacac ataaaaggaa tatgtaaaga
     9241 aaatcatggt tcgtctatgt ttatacttgg aaataaaaga aaagagtcag gtttgtctct
     9301 aatcgaagca tcattagtga ttattttggc tggactggtt attgtgctta ttcagtcagc
     9361 aatttctaaa attattaaga ctgctgttca tgaaaatgct ctgcatgata tttcatatgt
     9421 aatgtctaat attcaagata cattacagac gtcatgttat gaaaaaagtg atgatgtttg
     9481 tgagaaaaag ataacttcag taacattaaa gtttaacacg aaaaaaataa aggtagatat
     9541 aaaaaaaacg ggaaaatatg tggctgtgta tgttttgtat acactatcag acaacgaaga
     9601 ttttggcaga tatgaggatt tcggtagcat gttacttacg catgcaagtg cttattatgg
     9661 aagaattgat ggtaaaggcg ctgtgaattc tgttcttggg tggtggacag taaattctaa
     9721 cagcacaatc ttttcagggg gggataataa caagaatctt ttcttttaca ataatttccg
     9781 ggtgaatact ttatgatagg aaaagaaaaa ggtctttcat tgattgaagt gatgattggc
     9841 atcctttttt tttcagttat atctctttct gtatatgacc attacaacag aagcgcagaa
     9901 cagcattata ttaataatat ctctatcgtt atgtgcagat atgctgatgc gcttagctac
     9961 tatttacaag aagacgacaa agtaaacaat agtagtgtat cattatctga actggtgtct
    10021 tctggatatt taccatcgat gccagaagga gctagtgttg gcggatataa aattaaaatg
    10081 tatgtcgata acaaagataa tggtctgatt gcgttatatg gagatgagca ttattcactt
    10141 actattaaga gtatgaaacc atttctgggg attcgtggtg catctgtgga gggggataag
    10201 atcatatcta caggtggtta ttattccctg cctgtatccg gatttaccaa ttttagcact
    10261 gataatttaa gagggttaat gcttattcct tctattcaga catcaggaca aaattgtgac
    10321 agaggtggat atgatttttg aagggcgtca gcaaaagggg cagtctctta tcgaaatagc
    10381 aatggtcatt atcttgtctg ttctgtttat ttcaggtggt agttatatta tgaccctttt
    10441 taagactttt tctcaatctt gtgttgtagc aacagatatg aactctatca gaagtataat
    10501 agctagttcg actgttaaat ataaagataa aaaatacgat gcatcaatta taacaaataa
    10561 caatcggttc aataagtatc aggtctattt tggggatgat ggctatacgt taatatctgg
    10621 aggttctcca gttgagatag atgagtttta tattacctgc tacttagcaa aatataactg
    10681 tgtatggatt attaatcaaa acaataactt atatgcaaag catatgtctg ctgttctctc
    10741 accagagtta ttaagtacat atggcattag ttattcaggc gatagtggtg ttacagggct
    10801 tttgtttaaa cggtactagt tatgttatta ttctggtgtg gttttttttc atcaattgcg
    10861 ctcattttaa gtttgtctca aggttataat gaaaggttat atcttgatga acaagatcga
    10921 ataacaaaag aaaatatata tgtagattat ctttcagtga atgctgatgt tttatttgac
    10981 atttgcgcaa ctgaacaaaa cagatgcaat aatatacata caactctgcc ggaggagttt
    11041 gcagcacaac tgaaagagaa gttatctaat tatgataata gtattacacc tggattttgt
    11101 ataaataatg gggaaattat agtctataca tctcacaatt ttaatattga acgttacaaa
    11161 gacagttcat tgtttaatct ttccgacgtg cagagtgata ttaaaacagc ttgttcaata
    11221 catgattatt ctggtattgc cgttattaag atgctcaata aaaataaata gcgtcactgg
    11281 tactgtaagc ctcccagagt aatattgggt gccagatata ttactgatgg gaaataccac
    11341 ctccgatcat acattatgga taaggctata gcatagtcac tttatctgga gaagattaag
    11401 tataataagc catggatatt aggcgggcag acacaaacga cgtcagtatg agggttttgt
    11461 tttttattac aataatgctc aggtggtgac agacaataac ttatcgcaaa gaccccttca
    11521 gctctcggtt ccgggagtaa atactataca ttcttcgtca gtgattactg ctgagagtgt
    11581 tatgcacaat cgtgcagacc aatcggaacc tgtctgctga gcgatatcaa ctcggaagcg
    11641 tatctgcgcc atatttttaa tgtactgccg gaatgtactt ttaaccgtat tgataaatcc
    11701 cttctatgga acggagttat ctccaataaa taagcgtatt gcagtcggga aataatgctg
    11761 gcaatggtgt ttaattatat cgagtgtgat tacaatcgct ggcgtcgtca cagtgcctgt
    11821 ggcggtctca gccttgaaca atttgaaaaa cataatctcg cttagggtgt ccatagactg
    11881 gccccctgaa tctccagaca accagtatca cttaaataag tgatagtctt aatactagtt
    11941 tttagactag tcattggaga acagatgatt gatgtcttag ggccggagaa acgcagacgg
    12001 cgtaccacac aggaaaagat cgcaattgtt cagcagagct ttgaaccggg gatgacggtc
    12061 tcccccgttg cccggcaaca tggtgtagca gccagccagt tatttctctg gcgtaagcaa
    12121 taccaggaag gaagtcttac tgctgtggcc gccggagaac aggttgttcc tgcctctgaa
    12181 cttgctgccg tcatgaagca gattaaagaa ctccagcgcc tgctcggcaa gaaaacgatg
    12241 gaaaatgaac tcctcaaaga agccgttgaa tatggacggg taaaaaagtg gatagcgcac
    12301 gcgcccttat tgcccgggga tggggagtaa gcttagtcag ccgttgtctc cggatgtcgc
    12361 gtgcgcagtt gcacgtcatt ctcagacgaa ccgatgactg gatggatggc cgccgcagtc
    12421 gtcacactga tgatacggat gtgcttctcc gtatacacca tgttatcgga gagctgccca
    12481 cgtatggtta tcgtcgggta tgggcgctgc ttcgcagaca ggcagaactt gatggtatgc
    12541 ctgcgatcaa tgccaaacgt gtttaccgga tcatgcgcca gaatgcgctg ttgcttgggc
    12601 gaaaacctgc tgtaccgcca tcgaaacggg cacatacagg cagagtggcc gtgaaagaaa
    12661 gcaatcagcg atggtgctct gacgggttcg agttctgctg tgataacgga gagagactgc
    12721 gtgtcacgtt cgcgctggac tgctgtgatc gtgaggcact gcactgggcg gtgactaccg
    12781 gcggcttcaa cagtgaaaca gtacaggacg tcatgctggg agcggtggaa cgccgcttcg
    12841 gcaacgatct tccgtcgtct ccagtggagt ggctgacgga taatggttca tgctaccggg
    12901 ctaatgaaac acgccagttc gcccggatgt tgggacttga accgaagaac acggcggtgc
    12961 ggagtccgga gagtaacgga atagcagaga gcttcgtgaa aacgataaag cgtgacaaca
    13021 tcagtatcat gcccaaacca gacgggttaa cggcagcaaa gaaccttgca gaggcgttcg
    13081 agcattataa cgaatggcat ccgcatagtg cgctgggtta tcgctcgcca cgggaatatc
    13141 tgcggcagcg ggcttgtaat gggttaagtg ataacagatg tctggaaata taggggcaaa
    13201 tccagtccac attacgtggg taggatcagt tttctgcatt ccgtattggg tacaatgatg
    13261 ccggaaatct gcgaggctct atacctctct gattttcccc tttgtttatc actgtgctcc
    13321 agatttaatg ttgggggacg ttcatcttat tcccccgggg catgctaacg ccggcattta
    13381 cggtttctgt ctgttcttca gtaaaaataa aggaaacccc gctcatatag atactcccga
    13441 attattacaa aaaagaatta ttttttctgt gtg
//
LOCUS       DAZFJL010000124        13310 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFJL010000124 DAZFJL010000000
VERSION     DAZFJL010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.126"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            244..1341
                     /gene="mltA"
                     /locus_tag="ACHJ8C_003660"
     CDS             244..1341
                     /gene="mltA"
                     /locus_tag="ACHJ8C_003660"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO5553447.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGVDNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1418..2224
                     /gene="tcdA"
                     /locus_tag="ACHJ8C_003661"
     CDS             1418..2224
                     /gene="tcdA"
                     /locus_tag="ACHJ8C_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO5553448.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMNAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2275..2718)
                     /gene="csdE"
                     /locus_tag="ACHJ8C_003662"
     CDS             complement(2275..2718)
                     /gene="csdE"
                     /locus_tag="ACHJ8C_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO5553449.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPEELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2718..3923)
                     /gene="csdA"
                     /locus_tag="ACHJ8C_003663"
     CDS             complement(2718..3923)
                     /gene="csdA"
                     /locus_tag="ACHJ8C_003663"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO5553450.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNASDDKNIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKPELLEAMSPWLGGGKMVHEVSFEGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLTDYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4115..4342
                     /gene="ygdI"
                     /locus_tag="ACHJ8C_003664"
     CDS             4115..4342
                     /gene="ygdI"
                     /locus_tag="ACHJ8C_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO5553451.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4694..5611
                     /gene="gcvA"
                     /locus_tag="ACHJ8C_003665"
     CDS             4694..5611
                     /gene="gcvA"
                     /locus_tag="ACHJ8C_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO5553452.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5630..6025
                     /gene="ygdD"
                     /locus_tag="ACHJ8C_003666"
     CDS             5630..6025
                     /gene="ygdD"
                     /locus_tag="ACHJ8C_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO5553453.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6018..7118
                     /gene="rlmM"
                     /locus_tag="ACHJ8C_003667"
     CDS             6018..7118
                     /gene="rlmM"
                     /locus_tag="ACHJ8C_003667"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO5553454.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSSNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7162..7893)
                     /gene="fucR"
                     /locus_tag="ACHJ8C_003668"
     CDS             complement(7162..7893)
                     /gene="fucR"
                     /locus_tag="ACHJ8C_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO5553455.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIKLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVTTSLVTA"
     gene            complement(7951..8373)
                     /gene="fucU"
                     /locus_tag="ACHJ8C_003669"
     CDS             complement(7951..8373)
                     /gene="fucU"
                     /locus_tag="ACHJ8C_003669"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO5553456.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8375..9793)
                     /gene="fucK"
                     /locus_tag="ACHJ8C_003670"
     CDS             complement(8375..9793)
                     /gene="fucK"
                     /locus_tag="ACHJ8C_003670"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO5553457.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISTQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQILIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTTQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9902..11677)
                     /gene="fucI"
                     /locus_tag="ACHJ8C_003671"
     CDS             complement(9902..11677)
                     /gene="fucI"
                     /locus_tag="ACHJ8C_003671"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO5553458.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGKPTMKPHWEISQQEADACLTATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11710..13026)
                     /gene="fucP"
                     /locus_tag="ACHJ8C_003672"
     CDS             complement(11710..13026)
                     /gene="fucP"
                     /locus_tag="ACHJ8C_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO5553459.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     FGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         3219 a         3299 c         3498 g         3294 t
ORIGIN      
        1 gagcccgacg agctaccagg ctgctccacc ccgcgtccgt ggatgcgcac tatactctgc
       61 tcgcgttttc atgcaacctt ttttttaccc cgatcacgga tttggcatga ttttgaacaa
      121 aaacaaatca ctaaagccta ttttttgtgc aaaatttgcg tcagggagag gctgctcatt
      181 tcattaaacg cggtggtttg ttatcttcgt tgcgccttat tttttaacct gaagaagaga
      241 acaatgaaag gacgttgggt aaagtacctt cttatgggca cggttgtggc aatgcttgcc
      301 gcctgctctt ccaaaccaac cgatcgcgga cagcaatata aagacgggaa atttacccag
      361 cctttctctc tggtgaacca gccagatgcc gttggcgcgc cgattaatgc cggtgatttt
      421 gccgagcaaa ttaaccatat ccgtaattcg tcaccgcgtc tgtatggcaa ccagagtaat
      481 gtttataacg cggtgcaaga gtggctgcgc gcaggcggtg atacccgcaa tatgcgccag
      541 ttcggcattg atgcctggca gatggaaggt gtcgacaact atggtaacgt gcagtttaca
      601 ggctattaca cgccggtaat tcaggcgcgc catacccgtc agggcgagtt ccagtatccg
      661 atttatcgta tgccgccaaa acgtggtcgt ctgccgtctc gtgcggagat ctacgcagga
      721 gcgctgagtg ataaatatat tctcgcttac agtaactccc tgatggataa cttcattatg
      781 gatgtgcagg gtagcgggta tatcgacttt ggtgatggca gtccgcttaa ctttttcagc
      841 tatgcaggga aaaacggtca tgcctatcgc agcattggta aggtgctgat cgaccgtggc
      901 gaagtgaaaa aagaagatat gtcgatgcag gcgattcgtc actggggcga aacacacagt
      961 gaagcggagg ttcgcgagct gctggaacag aacccgtctt tcgtcttctt taaaccgcaa
     1021 tcttttgcac cggtgaaagg ggcaagtgcg gtgccgctgg ttggccgtgc ttctgttgcc
     1081 tccgatcgtt caattattcc gccaggtact accttgctgg cagaagtgcc gctgctggat
     1141 aataacggca aatttaacgg tcagtacgaa ttgcgtctga tggtggcgct ggatgtcggt
     1201 ggcgcaatca aaggccaaca cttcgatatc tatcaaggga tcgggccgga agccggacac
     1261 cgcgcaggtt ggtacaacca ctatggacgt gtctgggtgc tgaaaaccgc cccgggcgca
     1321 ggtaacgtct ttagcggctg atgtgatatt ctgtatgccg tttttacgga tatgacaggg
     1381 tgaggggtaa cctcaccctt tttaattctg aggtgctatg tctgtggtaa ttagtgatgc
     1441 atggcgtcag cgttttggtg gcacagcgcg tctgtatggt gaaaaagcat tgcaactgtt
     1501 tgctgacgcg catatttgtg tggttggtat cggtggtgtc ggttcctggg cggcggaagc
     1561 gctagcgcgc acggggattg gcgcaatcac gcttatcgat atggatgatg tgtgcgtcac
     1621 caataccaat cggcaaattc acgctctgcg cgataacgtc gggctggcaa aagcggaagt
     1681 catggcggag cgtattcgcc agattaaccc ggagtgccgt gtaacggtgg tggatgactt
     1741 cgtgacgccg gataacgtag cgcaatatat gaatgccggt tattcgtacg tgattgatgc
     1801 tattgatagt gtacggccca aagcggcgct gattgcttat tgtcggcgca ataaaatccc
     1861 gctggtcacg accggtggcg cgggtgggca gattgacccg acgcagattc aggttaccga
     1921 tctggcgaaa acgattcagg acccgttggc ggcgaagttg cgcgagcgcc tgaaaagcga
     1981 ttttggcgta gtgaaaaaca gtaaaggtaa gctcggcgtg gattgcgtgt tctctaccga
     2041 agcgctggtg tacccgcagt cagacggcac cgtctgtgcg atgaaagcca cggcggaagg
     2101 gccaaagcgg atggattgtg cctcaggatt tggcgcggcg acgatggtga ccgccacttt
     2161 tggctttgtt gcggtttctc atgcgctgaa gaagatgatg gcgaaagcgg cgcgtcaggg
     2221 gtaaacaatc aggtcggatg cgacgcgaac gtcttatccg acctggttct gtgcttaaac
     2281 ctgcttcgcc gcagcgataa tcgcctcgct taacgcattc aacccctggc tgcgtgaggc
     2341 gctaagttgc gcacgtaatc ccagctcatc aaacaatgcc agcggcgact gtgcctgcaa
     2401 ttcggcggcg gttttcccct caacggcagt caacaacacc gccaacaggc cgcgcacaat
     2461 gcgcccttca ctgtcgccaa agaaatgcat cttgccgttt tcagccacgg tatatcccag
     2521 ccagacgcgg ttttcgcatc cggcaatctc tttagcctgc gcttttaact cctctggcaa
     2581 agccggaagc tgttttccca gcatgatcaa ctggcgatat ttatcttccc attgcgtcaa
     2641 cggtgcgaag gtattgcgta acgtttctgc ggttacggtt gtgccgaacg gatgtccggc
     2701 gaattgcggg tttgtcatta atccaccaat aattccagcg cgcggtcaac ggcattcacc
     2761 agcgcatcca catcactctt tgtattatat ggcgcaaatg aggcgcgcag tgtgccggtt
     2821 acgcctaatt ctgccagtag cggctgagcg caatgctgcc cggcgcgcag ggcaataccg
     2881 tactccgcca gcagtgtcac catatcgctg tgatgaacac ccgcaaaatc aaaggccagc
     2941 aggctggaat cctggcagcg gaatgaacga aagcctggac gtttcgccag cgcttcttct
     3001 gcaagcgttg ctaagctacg gctccagctt tcggcctggt tgatatcgta atctgtcagc
     3061 cattccagcg ccgcgcttaa tcctatgacg ccagcgacat ttggcgttcc tgcttccagt
     3121 ttccacggcg cagattgagt cgtgaagcct tcaaaactca cttcgtgaac cattttgccg
     3181 ccgcccagcc agggcgacat cgcttccagc agttctggtt tgccatacag cacgccgatg
     3241 cctgtcgggc catacagttt gtgacctgaa aaagcataga aatcaatatc cagttgctga
     3301 acatccgcgg ggaaatgcac cgccccctga gcaccatcaa ccataaccac catcccggct
     3361 gaatgagcaa aagtaatcgc tctcgccaga tccgggcaac cgccagtgac gttcgacatc
     3421 tggcccaacg ccagaatccg actacgggga gtaatcagtt ctggcaacaa atcgacatcc
     3481 ggcagtcgct gcgcattaag cggcaatttc accactttgg cccccgtttg ttgggcgacc
     3541 atcagccagg ggacgaggtt ggcgtggtgt tctgccacac tgacaataat ttcatcgccc
     3601 ggttgcagac gcggacgggc atagcattgc gccaccatgt tgatggattc agtggtgccg
     3661 cgcgtccaga cgatattttt gtcatccgat gcattcagta attgtgccac tttttcccgc
     3721 gcagcttcgt aacgcgcggt caggcgttgg gcttcggcaa actggctgcg atggacgttt
     3781 ccggcgctca ggctataaaa ctgtcgggtg gcttcaacca cggcttcagg tttaagcgcg
     3841 gtcgcggcgc tgtcgagata gacgcccgca tcctgtagcg cgggaaactg ggcgcgaaac
     3901 tgcgcgggat taaaaacgtt catggtactc ctcggcttga tgcaccgatc gtgacgcaaa
     3961 attcactttg atacaaggag agtgctaacc atcagaatta tctggatatc ctggcgaagc
     4021 cgcgtcagta acatatgctg aatagtgtta ggcgttaatt ttagcctgtt aatcaatact
     4081 attaagattt attaatcgct ttaaggagaa gatgatgaaa aagactgccg caattatttc
     4141 tgcctgtatg ctgacttttg ccctgagcgc ctgttccggt tcgaactatg tgatgcacac
     4201 caatgacgga cgtaccatcg tctctgacgg caaaccacag actgataacg ataccggtat
     4261 gatttcgtat aaagacgcta atggcaacaa acagcagatc aaccgtactg acgtgaaaga
     4321 gatggtcgaa ctggatcagt aattcgcgat cgcaaggtaa aaaaaaagca ccgcaattag
     4381 gcggtgctac attaatcact atggacagac agggtaaatg tacaggaagt gaaaaaaggt
     4441 agctttgcta ccatggtctg aatcgcagac caattgcaaa cacaacaaca caacatcaca
     4501 accgtaagcc aaaagttcac cagaacacgc attccgataa aacttttcgt tccggctcag
     4561 gaagtgccgc cactataggt atttgctggt agaagctcaa cggacaattt ataatggctc
     4621 agattaaaaa aactaatagg ttacacagtg taatctaatt gttaaattca tttaacatca
     4681 aagtttaaaa gccatgtcta aacgattacc accgctaaat gccttacgag tttttgatgc
     4741 cgcagcacgc catttaagtt tcactcgcgc agcagaagag ctttttgtga cccaggccgc
     4801 agtaagtcat caaatcaagt ctcttgagga ttttctgggg ctaaaactgt tccgccgccg
     4861 taatcgttca ctcctgctga ccgaggaagg tcaaagctat ttcctcgata tcaaagagat
     4921 attttcgcaa ttaaccgaag cgacgcgtaa actccaggcc cgtagcgcta agggggcgtt
     4981 gacggtcagt ttactcccca gtttcgcgat tcattggttg gttccgcgac tttccagctt
     5041 taattcagct tatccgggaa ttgacgttcg aatccaggcg gttgatcgtc aggaagataa
     5101 gctggcggat gatgttgatg tggcgatatt ttatggtcgg ggcaactggc cggggctacg
     5161 ggtggaaaaa ctgtacgccg aatatttatt gccggtgtgc tcgccgctac tgctgactgg
     5221 cgaaaaaccc ttgaagaccc cggaagatct ggctaaacat acgttattac atgatgcgtc
     5281 acgccgtgac tggcagacat atacccgaca gttggggtta aatcatatca acgttcagca
     5341 agggccaatt tttagccata gcgccatggt gctgcaagcg gctatccacg ggcagggagt
     5401 ggcgctggca aataacgtga tggcgcaatc tgaaatcgag gccggacgtc ttgtttgccc
     5461 gtttaatgat gttctggtca gtaaaaacgc tttttatctg gtttgtcatg acagccaggc
     5521 agaactgggt aaaatagccg cctttcgcca atggatcctg gcgaaagccg ctgctgaaca
     5581 agaaaaattc cgctttcgtt atgaacaata atttacgtag ggtacgacca tgaccagccg
     5641 ttttatgctg attttcgccg ccattagcgg cttcattttt gtagctctgg gcgcttttgg
     5701 cgcgcatgtg ttaagtaaaa ccatgggggc cgttgagatg ggctggatcc agaccggcct
     5761 cgaataccag gcgtttcata cgctggcgat cttaggtctg gcggtggcaa tgcagcgtcg
     5821 catcagtatc tggttttact ggagtagcgt tttcctcgcg ttaggcacgg tgctgttcag
     5881 cggcagcctt tattgcctgg cgctgtccca tctgcgtttg tgggcgtttg tcacaccggt
     5941 tggcggcgtg agcttccttg cgggctgggc gttaatgtta gttggtgcta tccgtttaaa
     6001 gcgcaagggc gtaagtcatg aataaggttg tattgctgtg ccgtccgggc tttgaaaaag
     6061 agtgcgccgc agaaattacc gataaagccg gccagcggga aattttcggt tttgcccgcg
     6121 tgaaagagaa tgcgggttat gtcatttatg aatgttatca acctgatgat ggcgataagt
     6181 taatccgtga gctgccgttc agttcattaa tttttgcccg ccagtggttt gtagtggggg
     6241 aactcttgca gcatttgccg ccagaagatc gtattacccc cattgtcggc atgttacagg
     6301 gcgtagtaga gaagggcggt gaactgcgtg ttgaagttgc cgataccaac gaaagcaaag
     6361 agttgctgaa attctgccgt aaattcaccg tgccgctacg cgctgccttg cgtgatgcgg
     6421 gggtgctggc gaactatgaa acgccgaagc gtccggttgt gcatgtattc tttattgcac
     6481 caggctgttg ctatactggt tactcataca gcagcaataa ttcgccgttc tatatgggca
     6541 ttccgcgcct gaaatttccg gcagatgcgc cgagtcgttc cacgctcaaa ctggaagagg
     6601 catttcatgt gtttattccc gcggatgagt gggatgaacg cctggcgaac gggatgtggg
     6661 cggtggattt aggtgcttgc ccaggcggct ggacctacca actggtgaag cgcaacatgt
     6721 gggtttattc cgtcgacaac ggcccgatgg cgcaaagtct gatggatacc gggcaggtga
     6781 cgtggctgcg ggaagacggt ttcaaattcc gtccgacacg cagcaatatc tcctggatgg
     6841 tatgcgatat ggttgaaaaa ccggcgaaag ttgcggcatt aatggcgcag tggctggtta
     6901 atggctggtg ccgtgaaact atcttcaatc tcaaactgcc gatgaaaaaa cgctacgaag
     6961 aagtgtcaca caatctggcg tatattcagg cacagcttga tgaacatggc ataaatgctc
     7021 agattcaggc gcggcagttg tatcacgatc gcgaagaagt gacggtgcac gtccgccgta
     7081 tctgggctgc ggtgggtggt cgtcgcgacg agcgataaca ggtaaggccg gggcgtcgtc
     7141 tccggcctgc taccctttcc gtcaggctgt taccaaagaa gttgtaacct ggcgctcatc
     7201 agaaataatg tgcgttacct catccagatg cccgatgcgg gcttcccctg aacgattaaa
     7261 tttactttta tcaatcaata acaacgattg cgcggcacgt tttaataaca ttgatttgta
     7321 atcagcgttg atggcattgg agtcccacag tgcgccgctg ctatcgatcc cttcacaaga
     7381 aaaaataaac agatcgattt ccagcgattt aagttgggaa atcagcgagg gattgacgta
     7441 acagccatat ttgcgctcaa gtgtgccgcc ggaactgatc agtttaatgc gttcgcgttt
     7501 accgagttca tggcaaatcg gatggctatt ggtgaagacc tggatgttga tgtcaggcaa
     7561 ctggcgtgcc agataccagc aggttgaact ggcgtctaag gctatcacca tcccttcttc
     7621 aatccacgcg agcgcctcgc gcgcgatatc tgctttatgc gcataatggc ttttcagccg
     7681 gatgtgaaag ggatcgccac tgtcttgatt ttgacggtgg atatatttag cgcgcccatg
     7741 attgcgcaga attttaccct gcgtctgtaa ttcattgaga tcgcgacgaa tggtttcttt
     7801 actgaccttt agctgttcag agagagcttc cgtggtcagg ctggtatggt tcagcagcag
     7861 gtcgactatc gcttgctggc gtgccgcttt catatttttc accccatcat tcgataaccg
     7921 cccgcgtcat gcgggcgtac cggtatgaga ttacggtgtt accccttttt ttaaaagaat
     7981 attcccgtac ttcgctcgtt cgcctgtgat aacgatcgca aaggcttttt gcgcccgttc
     8041 ataaaacgca aaacgattga tgcggatgat gtcaggacag ggggcttgta gtgaaagcgc
     8101 attacgataa cgtcgttcta cttcaggatc gagagtgtca ccctctaccg ccgccatcat
     8161 caccagcggt ggtgcataac tgtccagctc aaataacggg ataatcgcct ggagcaagtc
     8221 gctcaccaac aggccatcag cgcggatcac ctgcggtccc atcgaatggg cgggaaagtg
     8281 agcatcggaa aaaataattt catctccatg ccccatctct gccagcactt tcaatagttc
     8341 gggagaaatt aacggcgaaa ttgttttcag catttcacac ctcctctata aattcaggtt
     8401 cagtttgcgg gtagaaataa cggtactgat aatgaatctg cgcgcgggct tcttccgggc
     8461 tgttaaattc ccctacgcca taccaaccaa acagcgcagc tcctgcgacg gtcgtttcgg
     8521 catcgtcgag aacttttacc ggaatatcaa gcatattggc tttaatctga ttccacaatg
     8581 tgttgcgact tcctccgccg actaacaata attcagaggc tttaaagtgc ccgatttttt
     8641 ccagcatctg tagattgcgc tgtaattgcg tggttaaccc ttccagcgcc gcgcgataga
     8701 aatgcccccg cgtggtatta agcgttacgc cttgccagcc agcgttctgg cacgacaata
     8761 aatcacactg cattttcact ccatctgcgc caggcgcgat caggcgagct tcttcaatca
     8821 atatttgcca gggagtttca gctgtccaga acagttttct cacccattcc agcacgccgg
     8881 atgccagcca ttgcatacct gggttataca accctgcctg gctatccagt tcgcaagtag
     8941 aaccggcgta ctgacttaac agcgaagtat caacctgggc gctgcggacc attaaaattt
     9001 cccatgtacc ggaagagagc acgggttcat tttgttcggc accagcgcca aaaagggcga
     9061 actgggtatc gtgacctgcg gaaatcaccg gtatgccaac gggtaagccg agcattgctg
     9121 cggcgctgtt ctgtagcgta ccaatctgtt cacccgcttc caccagacga gggaagagtc
     9181 ggcgtggaat accggtggct tgtaaaattt gcggactgaa atcgcgttgc tggatatcca
     9241 gcatctggct ggttccggcc atcgtaatat cagtagtgaa ttcgccggtt aaacggtggt
     9301 taatcagcga cgaaataaag agccaggcgt gcgcgcgttc cagcagttgt ggatgatttt
     9361 ctttcaacca caccaactta tataacgtat tgaaactaaa ggctccgact ccagaaatag
     9421 cctgcaactg ctgtgtggag atcaaccgtt caatattgtc cattaccgcg gctgttcgcg
     9481 gacatttcca gctaatgatc ggatagagca gattgccttg cttatctacc agagcgccat
     9541 ccacaccaaa ggtggtgacg gcgataccgc ggatgtggca atcagtcagt tcactattga
     9601 tttgccgaca gcaatcagca aagcgttgca aaatggcgtc taaagaccac tggtgccagg
     9661 tgttgttttc catcgcgata tcgctggcat taggcgttga ggcgcgggca acaatttttc
     9721 cctgccgatt aaccgcgatg gccctgacat tggtcgcgcc acagtcgagt accaggataa
     9781 cttcttgttt cataatcgct cctgcaatat agccggataa cattgcttat ccggctaacc
     9841 actcttgctt gttcaggccc ggtagccgga gcgaccgggc atcacatcag ggagtcatgt
     9901 attaacgctt gtacaacggg ccgtagttct ggcaagcgcg gtaatcctgg ccttcaatat
     9961 ccatgccgtg cgcggcccag gcagaaggac gatacacttt ggtctcttca acgttgtgca
    10021 tacataccgg gatacgcagc atggaggcaa gagtgataaa gtctgcgcca acgtggccga
    10081 tggtcagaac cccgtggtta gcgccccagt tcgccattac cgagtacaca tccgtaaacg
    10141 gccctttacc ggtgaggcgc ggtgcaaacc aggtggttgg ccaggttgag ttggtgcgtt
    10201 tgttgaggat gtcatgcaca tccttcggca attccacgct ccagccttcc gcgatttgca
    10261 gtaccggtcc caggcctttg atgatgttga cacgagtcat ggtgaacggg acgccgcctt
    10321 cggtaaggaa gcgggaagag taaccgccgc cacggaagta ttcgtggatc gccgggcacc
    10381 attcggtagc ggtgaggcaa gcgtcagcct cttgctgaga gatttcccag tgcggcttca
    10441 tcgtcggttt accttcgctg tcgcgttgtt tacaggagcc gtccagcgca gcagaaccgg
    10501 agttgatcaa atggatgatg ccgtgttctg ccagcccgtc cagtttatgc cccgttacac
    10561 gctcaattgc ttctggtgac cagtaggtac ggacatcggc aaatacctga gcggtgccgg
    10621 tgagctggtg gcccattaac attgccacgc cgttaagact gtcgttttcg gtcgccacga
    10681 caaagggttc gcgcacgcca ttccagtcaa atgaactgtt gaggatcgct tcggcggtgt
    10741 cgccattggg atattgatcg gtccagtgac gttgcccctg gaagcccgca gcgatggcgt
    10801 tgtagccaag tgattcttcc acgcggccaa tatcggccag tttgctgttg ccttgcatca
    10861 tgtcgcggat acacatcgcc atcagtaaac tttcgcgcag aactgcgcgg ctttgctcgg
    10921 catttcgttg atactgtttg ttattttcat cttcgccata gcggaagttt ttatcagccc
    10981 aggccagcgc catttccagt tcggcttcgt cgtaaatctt ctgatcgata cggcgacgca
    11041 gttcggtcat atccaccgcc tggactttca ttcccagcca ggattcaaag aagttgtgat
    11101 caacaataga accggcgata cccatcgaaa cgccgcctag cgacagatag cttttacctt
    11161 tcatgctggc gacggccaaa ccggcgcggg caaaacgcag cagtttttct tcaacatcgg
    11221 caggaatcga tgtgtcatcg gcatcctgaa cgtcatgacc gtaaatggag aatgccggga
    11281 tgcctttctg gctgtgagct gccagagctg ctgccaggta aacagcgccg ggacgttcag
    11341 tgccgttaaa gccccaaatg gctttcgggc gggttggatc catatcgatg gtttcactgc
    11401 catagcacca gcaaggggtt accgtaatgg tgaggcctac attctgactg ctgaattttt
    11461 cttcgcaagc agcggcttca gccatacccg cgatacaggt atcggaaatg acacactcga
    11521 cggcagctcc acaggcatgg cgcagtttct cggtcagcag tgcggccgtg gctttcgcca
    11581 tattcattgt ttgttcttca agcgactcac gaacacccat gcgacgaccg tcaataaccg
    11641 ggcggatacc aattttcggt aagctgattt ttttcattac agattcctca ctttattcgg
    11701 aaaatatgtt cagttagttg ccgtttgaga acggaaacgg gcaaagataa agatgactgc
    11761 gaagcagagt gcggggatca gttcagctgt ggggatgttg cccgcagcgt cactgacaaa
    11821 acccatgacc ggagtgacaa taccgccgcc aataatggtc ataacgatga aggacgaacc
    11881 gtatttggtg tcctggccga gattcttaat gcccagcgag aagattgttg ggtactgaat
    11941 cgacataaag gcactgcata aagtcagggc tattaaaccc acatgaccgc cagcgaaggc
    12001 tgagatcagg cacagtgcca tagcaattaa tgcgtaggct gccaggactt tgtgtggtgc
    12061 gaagcgactg atgagccagg taccggtgaa acgaccaata aagaagcaca ccatggttcc
    12121 ggttaaatag ttagcggcaa agcctgctgt catacctgga atttcttcta cagcgtagcg
    12181 aatcaaatag ctccagcagg ccgtttgtgc gccaacgtag cagaattgcg ctaatactgc
    12241 ccagcgccag tggcgaatac gcgccaggcg agaaagcgat gcggagaacg atccttgttt
    12301 ggcgtcactg tgattatcac tctgcaatgc cgggaatttc gtcagcatga tcagcagggc
    12361 gaccagtaac acgatagcca cgatgatcat ataaggtgtc tgtaccgata ataccaggct
    12421 gtgtttatac gcactcaatt gctctggaga cattttatcg agaacgtctt gcgattgatg
    12481 tggcacgtta gacaaaataa gactttgccc aaagacaacc gcgataattg cgccaaatga
    12541 gttaaatgtt tgcgcaagat ttaagcggaa gtgaccactg ctttccggcc ctaataccgt
    12601 aacaaaaggg tttgcggcag tttccagaca acctaatccg gctgcaataa taaataggcc
    12661 aactaaaaac aaggtgtagt tcattatttc tgcggcgggc cagaataatg cagcgccgaa
    12721 ggcatataaa aacaatccgg taataatccc tgctttataa ctgagttttt tcatcaatat
    12781 cccggcaggg attgggataa tgaaataacc aaagtaaaag gccgattgga tcaggccagc
    12841 ctggaaattt gtcagcgtaa aagcctgctg gaattgaggc aataaaatgt cgttaaggtt
    12901 attggctacc gcccaaagaa aaaacagtga gcacagcagt gcaaatggaa taatgtaact
    12961 tctgctttgc cctgcatctt tatctaccgc acggtaactc tgcgtttgta ttgatgtgtt
    13021 tcccatagca tcctcttagg ttcagaagct taagcacgta atcggttatg ttttcaaaag
    13081 tgcaaaacca gcgagttcgg gcttcatatc agtgaatatt tgcctgtctg aaatgaactc
    13141 atggattcat tttcaaataa aaaacgctct gtaatatgac ggcggtcaca cttattagcc
    13201 ggaaggtgac tttatgtgac taccatcact ttaataatcg aaatttcatg cccgtttcag
    13261 aaaataaatc ataaaaaaca caaaccagat gaccgtatta attgtttgtg
//
LOCUS       DAZFJL010000125        13168 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFJL010000125 DAZFJL010000000
VERSION     DAZFJL010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.127"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(140..2407)
                     /gene="adiA"
                     /locus_tag="ACHJ8C_003673"
     CDS             complement(140..2407)
                     /gene="adiA"
                     /locus_tag="ACHJ8C_003673"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO5553460.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESERYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKINESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFSTHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYHFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2606..3538)
                     /gene="melR"
                     /locus_tag="ACHJ8C_003674"
     CDS             complement(2606..3538)
                     /gene="melR"
                     /locus_tag="ACHJ8C_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO5553461.1"
                     /translation="MFQERESSMNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPH
                     IMPTSHWHGQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMA
                     IFNLPMHLFLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQL
                     AIDEIGLMLKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTI
                     NDVAEHVKLNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGF
                     RSSSRFYSTFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3797..5152
                     /gene="melA"
                     /locus_tag="ACHJ8C_003675"
     CDS             3797..5152
                     /gene="melA"
                     /locus_tag="ACHJ8C_003675"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO5553462.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTVEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKAGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            complement(5149..5799)
                     /gene="yjdF"
                     /locus_tag="ACHJ8C_003676"
     CDS             complement(5149..5799)
                     /gene="yjdF"
                     /locus_tag="ACHJ8C_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO5553463.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITRAPEAITP"
     gene            complement(5922..7568)
                     /gene="fumB"
                     /locus_tag="ACHJ8C_003677"
     CDS             complement(5922..7568)
                     /gene="fumB"
                     /locus_tag="ACHJ8C_003677"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO5553464.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDIQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRKAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(7646..8986)
                     /gene="dcuB"
                     /locus_tag="ACHJ8C_003678"
     CDS             complement(7646..8986)
                     /gene="dcuB"
                     /locus_tag="ACHJ8C_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO5553465.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(9557..10276)
                     /gene="dcuR"
                     /locus_tag="ACHJ8C_003679"
     CDS             complement(9557..10276)
                     /gene="dcuR"
                     /locus_tag="ACHJ8C_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO5553466.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAVTIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(10273..11904)
                     /gene="dcuS"
                     /locus_tag="ACHJ8C_003680"
     CDS             complement(10273..11904)
                     /gene="dcuS"
                     /locus_tag="ACHJ8C_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO5553467.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MHSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVNVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            12085..12315
                     /gene="yjdI"
                     /locus_tag="ACHJ8C_003681"
     CDS             12085..12315
                     /gene="yjdI"
                     /locus_tag="ACHJ8C_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO5553468.1"
                     /translation="MDQALLDGGYRCYTGENIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVTTVVKVIDMCPSGALKYRHK"
     gene            12327..12599
                     /gene="yjdJ"
                     /locus_tag="ACHJ8C_003682"
     CDS             12327..12599
                     /gene="yjdJ"
                     /locus_tag="ACHJ8C_003682"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5553469.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            12828..13124
                     /gene="ghoS"
                     /locus_tag="ACHJ8C_003683"
     CDS             12828..13124
                     /gene="ghoS"
                     /locus_tag="ACHJ8C_003683"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO5553470.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLSIMTVDDYFRQFYK"
BASE COUNT         3225 a         3157 c         3436 g         3350 t
ORIGIN      
        1 aaagcgcggg gtgggaccgc gcaaaaagca tgacattttt tgtttgcagc aatttttatg
       61 aaaacgttgc catacttaaa aggcctcaag ccaggcggcg taatgttatt taacaattac
      121 gccttcagcg gaatagtggt tacgctttca cacacataac gtggtaaata ccgtcaataa
      181 tttctgtccc ttctgtttcg tgttcaaatc cagggaaatg atggtcccag gattgcagcg
      241 agcgtaaata acttacttgc ggactgtttt tatcgccgaa gttttcacca gacagcagca
      301 tcgggattcc tggcggatac gggataactg agtttgccgc gatacgtcct ggcagatttt
      361 caatggatac cagttcgaca ttgttgtcga caatcgcgtt gtacgcttca cgcggggtga
      421 tttctgccac cggtaggccg gaataggctt cattcaaccg tgcgccaggg ttgttttctt
      481 tcagccaggc aaacatggtg tcacccagat cgtgaatccc catgttcgcg taagtgtcag
      541 gatattgttc aacaagttcc ggcatcacct gcgccagcgg tgtgttggca tcatagtggc
      601 gtttgaagga gcaaagggtg ttaaccagag tcccccattt cccacgggtc acgcccatag
      661 agaacaggaa cataatctgg aagtcagtgg tacgggtagg cacaatgccg tggcgaccaa
      721 gccaggctgt gaccagcgcc gccggaacac cggtttcttc cagttcacca tcttcaccca
      781 ttcccggagc aaggatgctg actttaatcg ggtcgagcat actccagtta tccggaatat
      841 ctttgaagcc gtgccagctt tcgcccggat gcatgaccca gcagtcctga acggtggtca
      901 gcagtttggt tggtgcgtca gcaaagtgat aggttttgcc ggtttgtggg tcggtgacga
      961 cttctttgtt ccacggtttg aagaaccagc taccgtcagc ggtgaactct ttatacagcc
     1021 gcgccatcgc ctgacggaaa tcaaccgctt cgtcgatcac ttcctgggtc agtgacaggc
     1081 cgctgttgcc gtccatcatc gacactgcca cgtcgttgga tgcgcagatg gcatacagcg
     1141 gggaggtggt ggcatgcatc atgtaggcct ggttgaagcg ggagaagtta atcgccccac
     1201 gaccttcacg tacatgaata taagaggcct gtgacagcgc attcagcagt ttgtgggtgg
     1261 agtgggtgga gaaaacggta ggaccgttgt gatcgccagg ttcgccgcgc atggcatagt
     1321 gatcggcata gatcgggttg aaacgtgcat agccgtacca ggcttcgtca aagtgcagac
     1381 gatcggaggt tttttccagc agatcctgtg cttctttagc gttataacac acgccgtcat
     1441 aggtgcagtt ggtcaccacg cagtaagacg gtttttgccc ggctttgtct ttagttagcg
     1501 ggctttcatt gattttcttc tgcaaggttt caggttgcat ttcctgcgga tagattggcc
     1561 cgataatgcc gtagcggttg cggcttggca ccatgtagac cggtttcgcg cctgtcagca
     1621 tcaagccttg ttcgatggat ttatggcagt tacggtcaac gaccacgaca tcgttatcgg
     1681 tcatgcaagc ctgcatgatg gtgcggttag agccggaagt accgacgact accgaccagg
     1741 agcgatcggc accaaatacg cgtgcggcat atctttcgct ttcgccaaat gcgccagtat
     1801 ggtcaagcaa agaaccgagg gaagtgcgtt cgatgcccat gtcggtgcgg aacagatttt
     1861 caccataata gtcatggtag aaacgtccgg cgggtgtttt ggtaaaacca acgccgccct
     1921 ggtggcctgg cgctgcccag gaatattcat ggatgtcact atatttcatc agtgcgctga
     1981 acagtggcgg caatagctgc tggcggtagc gggtcatcgc ggcaacggcg cgtccggcga
     2041 taaagtcggc ggtatcttcc agaatccagg cgaattcatc gacaagctcc agcagatccc
     2101 ggtccattgc ggcgagggct ttttcccgat cgcccaacag gaagaccggc acgttttgtt
     2161 ggcgctcatg aagcttaccg atcaattgtc tgacgttttg atgttcgtcc ggatgttcca
     2221 tttgatagct gaacatcagg cagtcaatgg cttcgtttga agagagaatg gcaaaaccat
     2281 catcaaagga ggtggattta atcacggtaa cattttgctg gcttaaagca tctgccagac
     2341 gctcaacggc gttaccgacc caagtgtctt gatggagaaa ctcgctttca acaattaata
     2401 ctttcatcat tgcttacccg gttatgaagg aatcttcgtt gcgggcaagt atcttccggc
     2461 cttttcgggc tgtaaagcgc gtgaaaaaca aacatttttg aaatatttat ttttataaac
     2521 aacacgttat gtgattattt taaattattt ttactgctta ttatcgacgg ctaaactatt
     2581 tttttggctg atactgatat cgtctttagc cgggaaacgt ctggcggcgc tgttggctaa
     2641 gtttgcggta ttgttgcggc gacatgccga catatttgcc gaacgtgctg taaaaacggc
     2701 tactcgaacg aaagcctgcc gtcagggcaa tatcgagaat acttttatcg gtatcgctca
     2761 gtaacgcgcg aacgtggttg atgcgcatcg cggttatgta ctgtttcatc gtcaattgca
     2821 tgacccgctg gaatatcccc attgcatagt tggcgttaag tttgacgtgc tcagccacat
     2881 cgttgatggt cagcgcctga tcatagtttt cggcaataaa gcccagcatc tggctgacat
     2941 aaaattgcgc atggcgcgag acgctgtttt tgtgtgtgcg cgaggtttta ttgaccagaa
     3001 tcggttccca gccagagagg ctaaatcgct tgagcatcag gccaatttca tcaatggcga
     3061 gctggcgaat ttgctcgttc ggactgttta attcctgctg ccagcggcgc acttcaaacg
     3121 ggctaagttg ctgtgtcgcc agtgatttga tcaccatgcc gtgagtaacg tggttaatca
     3181 ggtctttatc cagcggccag gagagaaaca gatgcatcgg cagattaaaa atcgccatgc
     3241 tctgacaggt tccggtatct gttagttggt gcggtgtaca ggcccagaac agcgtgatat
     3301 gaccctgatt gatattcact ttttcattgt tgatcaggta ttccacatcg ccatcgaaag
     3361 gcacattcac ttcgacctga ccatgccagt ggctggtggg catgatatgc ggtgcgcgaa
     3421 actcaatttc catccgctgg tattctgaat acagcgacag tgggctacgg gtctgctttt
     3481 cgtcgctgct gcacataaac gtatctgtat tcatggatga ctctctttcc tggaatatca
     3541 gaattatggc aggagtgagg gaggatgact gcgaacaggc gcacggtttt atttctctcc
     3601 ctggaggtgc gaggagagcg gatgagaaga ctggggtcga ttattcagct tttcaggtaa
     3661 tttattccca taaactcaga tctattgatg tgccacttag gatctgagtt tttgggaatg
     3721 ctcaaccggg aaaccggagg ttttctgcga tatcgcctgc catgatgaag tcattcaagc
     3781 aagccaggag atctgcatga tgtctgcacc taaaattaca tttatcggcg ctggttcgac
     3841 gattttcgtt aaaaatattc ttggtgatgt gttccatcgt gaggcgctga aaacggcgca
     3901 tattgccctg atggatatcg atcccacccg cctggaagag tcgcacattg tggtgcgtaa
     3961 gctgatggat tcagcagggg ccagcggcaa aatcacctgc cacacccaac agaaagaagc
     4021 cttacaggat gctgattttg tggtggtggc atttcagatt ggcggttatg aaccttgcac
     4081 ggtgactgat ttcgaggtct gtaagcggca tggtctggaa caaaccattg ccgatacgct
     4141 ggggccgggc ggcattatgc gtgcgctacg taccattccg cacctgtggc agatttgcga
     4201 ggacatgacg gaagtctgcc ccgatgccac catgctcaac tacgttaacc caatggcgat
     4261 gaatacctgg gcgatgtatg cacgctatcc gcatatcaag caggtagggc tgtgccattc
     4321 ggtgcaggga acggtggaag agctggcgcg tgacctcaat atcgatccgg ctacgctgcg
     4381 ttaccgttgc gcaggtatca accatatggc gttttacctg gaactggagc gcaaaaccgc
     4441 cgacggcagt tacgtgaatc tctacccgga actgctggcg gcttatgaag cagggcaggc
     4501 accgaagcca aatattcatg gtaatactcg ctgccagaat attgtgcgtt acgaaatgtt
     4561 caaaaagctg ggctacttcg tcacagaatc gtcagaacat tttgctgagt acacaccgtg
     4621 gtttattaag ccaggtcgtg aggatttgat tgagcgttat aaagtaccgc tggatgagta
     4681 cccgaaacgc tgcgtcgagc agctggcgaa ctggcataaa gagctggagg agtataaaaa
     4741 cgcctcccgg attgatatta aaccgtcacg ggaatatgcc agcacaatca tgaacgctat
     4801 ctggactggc gagccgagtg tgatttatgg caatgtccgt aacgatggct tgattgataa
     4861 cctgccacaa ggatgttgcg tggaagtggc ctgtctggtt gatgctaatg gcattcagcc
     4921 gaccaaagcc ggtacgctac cttcgcatct ggccgccctg atgcaaacca acatcaacgt
     4981 acagacgctg ctgacagaag ctattcttac ggaaaatcgc gaccgtgttt accacgccgc
     5041 gatgatggac ccgcatactg ccgccgtgct gggcattgac gaaatatatg ctcttgttga
     5101 cgacctgatt gccgcccacg gcgactggct gccaggctgg ttgcaccgtt aaggggtaat
     5161 cgcttccggc gctctggtga ttaagccaaa gcgccgtagc tggcggcaat gaaagcgggc
     5221 gaggaatatc accgtcgtta atgcgccaag cagcgcgcag aacatatcgg attgcgtgtc
     5281 ccattggtcg ccctgggtgc cgagaaaatc atccgctccc tggcccattg ctagtgccgc
     5341 ccaccactcg attaattcat acatcgcgct tatcgccagc gccacgcaac agaccagaaa
     5401 cgccaccatc ttacgtccgc gaacgtacat cccgcgaacg agaatttctc gcgccaccag
     5461 tgcaggcacc agcccctgga aaaaatgccc cagtttgtca tacggattac ggctcaaccc
     5521 cagccattcc tgcacctcaa aaccaacggg gactttcgcg taggtgtatt gcccgccgac
     5581 catcaggatg atggcgtgaa agaaaatgag cgtatagagc agcggcgtta acgggtaacg
     5641 tttagcggtg gcaagcagta gcggcacgac aataatcacc ggtgtcactt ccatcagcca
     5701 ggtgagtttg tcatgggccg aaatgccggt ataaatcagg attaacgtta gcgccagtgc
     5761 gccggtgtta agaattaacg gcttgagtgt gcgggtcatg gtgagttcgc aagtcaggga
     5821 aaatactgac tattgaccag taagcgtgaa attacaatcg caaaagaggt ggcccagggg
     5881 gatcacctgg cagcatgctg ccaggcgctg ggccgaagag gttacttagt gcagttcgcg
     5941 cactgtttgt tgacgatctg ctggaagaag tcgttacctt tgtcatcgac caggataaac
     6001 gccgggaaat cttctacttc gattttccag atagcttcca tacccagctc cggataagcg
     6061 acgcattcca gatgcttgat gctctgctgc gccagtaccg ccgccggacc gccgatgcta
     6121 ccgaggtaga agccgccgtg tttatggcac gcgtcggtaa cctgctggct gcggttacct
     6181 ttcgccagca tgatcatgct gccgccgtgg gattgcagca gatccacgta ggagtccata
     6241 cgacctgcgg tggttgggcc aagtgaacct gatggataac cggcaggcgt tttcgccgga
     6301 cccgcgtagt agatcgggtg atctttgatg tactgcggca gttctttacc ggcgtcaatc
     6361 agctctttca gcttggcgtg tgcaatatcg cgacccacga taatggtgcc ggtgagcgac
     6421 aagcgggtgg ataccgggta ttgcgaaagc tgggcaagga tctctttcat cgggcggtta
     6481 aggtcgactt tcactgcttc gccctcaccg gccttgcgca gttcttgtgg aatgtactgg
     6541 cctgggttgt gttccagttt ttcgatccag atcccttcgc ggttgatttt cgctttaatg
     6601 ttacggtcag cggagcagga gacgcccatg cctaccgggc aggatgcgcc gtgacgtggc
     6661 agacggataa cgcggatgtc gtgcgcgaag tatttaccgc caaactgcgc gccaagaccg
     6721 agtttctggg cctcttccag cagttcctgt tccagctgga tatcgcggaa cgcctgacca
     6781 tgttcgttcc cttccgtcgg cagttcatcg taatagtgag cgcttgctaa cttgacggtt
     6841 ttcaggttgg tttccgcaga cgtaccgcca atcacaaacg cgatatggta cggcgggcag
     6901 gctgcagtac cgagggtacg cattttctcg acgaggaagt ttttcagttt gccgggcgtc
     6961 agcagggctt tggtttcctg gtagagatac gttttgttgg cagagccgcc gcctttagcg
     7021 acacagagga atttgtactc atcgccatct accgcgtaca ggtcgatttg cgcaggcagg
     7081 ttcgtgccgg tgttgacctc tttatacatg tccagcggcg cgttctgtga atagcgcagg
     7141 ttatcttcga tataggtgtt atagacgcct ttcgacagcg cttcttcatc accgccaccg
     7201 gtccacacgc gctgaccttt tttaccgacg atgatcgcgg tgccggtatc ctggcaggtc
     7261 ggcagcacgc ctttggcggc gatttcggag tttcttaaga attgcagcgc cacgtacttg
     7321 tcgttttcgc tggcttctgg atcgtgaaga atagccgcaa cctgtttctg gtgtgccggg
     7381 cggagcatga aagaagcgtc gtgaaaggct tgctgcgcca gcagggtcag ggcttctggt
     7441 tccactttca ggatggtttc gccgtcgaag tcggcaacgc taacgtaatc ggaagtgagt
     7501 agatagtatt cggtattgtc tttccccatc gggaaaggtg cctggtagat aaagggtttg
     7561 tttgacatag cttccagcct gtaactctgt atttgttatt cgaaaatggc gtgccgcgta
     7621 gttacgggcg gcacgcaaag tgcatttata agaacccgta catcgcggcg aagatccagc
     7681 cgaagacgca cgatacgctc acaccaatca accccggcag aataaagctg tggttgatga
     7741 cgaagcgacc gatgtgggtg gtgccggaac ggtcaaactg aatcgctgcc agatcgctcg
     7801 gataagtcgg caggatgtaa taaccgtagc aagccggtgc tgaagccacg atgtatgccg
     7861 gatcaacgcc gatcgccagc gcgaccggaa caatcgccgc cagcgctgca gcctgagagt
     7921 ttacaaactt ggaaaccagc agcagaacaa tggcataggc ccacggatac tctttcacca
     7981 tttcacccag tacgccctga atttcagaca tatgcgcacc gaacatggtt tcagccatcc
     8041 atgcgatacc gtacaccgcc acgatggcga tcataccgga acggaaaact tcgttttttg
     8101 agatagacgc gggattggtt ttggtcagga taataatcag cgccccggtc agcagcataa
     8161 acatctgaat aaccagtacc atcgacagcg gtttgccgcc gaaggatgga cgcaggtccg
     8221 aatcagcacc aagaagggcg actacagcga ttgccccgag gaagatccac attgccagcc
     8281 agttgctttt cggcagtttt ttatccagca gcgtcgcggt atcaccgtaa acatactcac
     8341 ggttttccgg tacggagatg aatttttgga actcttcgtc tttatccaga tctttaccgc
     8401 ggaaccagct gaagataccg atcgccagga taccgattaa cgtcgatgga atggtgattg
     8461 ccagcaggtc gaggaactca agatggcgac catcaaaggt gacattaccc agcatcgcaa
     8521 ccaaagatac gaccgcaacc gacaccggac tggcgataat ccccatctgt gcaccgatag
     8581 aacttgccgc catcggacgt tccggacgga tgttgttctt aatagcaacg tcgtagatga
     8641 tcggcagaat ggtgtaaacc acatgacccg taccgcaaag aatggtcagc gtacaggtca
     8701 caaacggcgc gacaattgag acatatttcg ggttgcgacg cagcagcttc tcggcaattt
     8761 gcagcatgac atcaagaccg cccgaagctt gcaaggtcgc cgatgccgcc accaccgcaa
     8821 tgataaccag catgacatca accggtggtt tacctggctg aaggtggaag acgaagacca
     8881 gaatgaccag accgataccg cctaataaac ccagcgcgat accccccttt ctggcaccat
     8941 aaaacagaca tatcagtatt atgataagtt ggatagtaaa taacatgtgt gaaccctcgc
     9001 gataatccta tttaaatttt tgctgaatag atcacagtca cgttctgttt tgtatgaact
     9061 gttttcagag cctgactaaa tatccgtttg gtcgttaccg gcttgagcaa atacctcaca
     9121 gtgaatattg gctgattaaa gcagcggcaa atttatgcac tgtgataagc ggctttttca
     9181 gagagaaaga ctctctggcg atgggtttat agtttgtaca gcgcagatgt taatgttttg
     9241 tttgctgtta tcttattgat atttatcggt ctgttttttg ttctcagggc aggtatcatt
     9301 aggtttatag attaatctga tctacccatt tgtgggtaaa aatacacata atgtgggtga
     9361 cataatagtt aattaacttt tgttagcgtt ttgaaattaa aaacaccgtt catctgaaga
     9421 gatattaatt tttagcgatg atggagggat aattatattt gatctggcac aagttttact
     9481 gatgaacgat gtaacttgtg ccagggggat tttgcattac ggtaataatt attacttaca
     9541 gataaccagc gatccgttat tggcaatatt gtttcagtag tgagtagtgt tctgcctgaa
     9601 tacggtaacg gtaaaccgga cgccccgtga cgccataatg gatactggtg aacaatatgt
     9661 ggcagttgac cagccagata aggtatttac ggcaggaaac acgcgagatg ttaacctcgt
     9721 tagccagctc gtcggttgaa aactcatagt cctgatgcgc gtcaatccac tggcacagcg
     9781 tgcgtaacgt ctgcggcgtt aagccttttg gcaagcgacg aggatcctgc tcgttggagc
     9841 tgctgccgtg gattagctga tcaagctcgg cctggtcata atactgatgt ttttccagcg
     9901 ccattttctt ttgccgccag ccggtgagcg cctcttcaaa gcgggaagcc tggaagggtt
     9961 tgatcaggta atccacaaca ccgtaatgca gcgaatcttt aatggttact gcatcggcag
    10021 cggaggagat gacaatcaca tcacttttgc aacgcgcgtt atgcaggaca ggcagtaaat
    10081 cgagcccgtt ctctttttgc atatagatat ctagcaatat caggtcgata ggcgtatcgc
    10141 tattgaagat aatctctttg gctttctcca gcgtcgaagc cgttccacag cattgaaagc
    10201 ccgggatttg tgccacgtat cggcgattca gctccgcgac cattgcgtca tcatcgataa
    10261 ttaatacatt gatcatctgt tcgacctctc cccgtcccag ggtatctgga caaaaaattg
    10321 tgtgaaaatc ccgggttccg attccacggc gatgctgccg ccgagatttt ctacctgttg
    10381 tttgacaagt gctaaaccga cgcctcgctc gcttcctttt gtcgagacac ctttgtcaaa
    10441 aatgtgatcg attttgtcgg gtgcgatccc cggtccatca tcgttaactt cgcagtgcag
    10501 ccagccgtga cggtagtgca atgttacgct aatttcgcca ccgggttccg gccctaatgc
    10561 ctccagcgcg ttttctatca gatttcccaa cgtggtaatc agcgtcgcga cctgatcctc
    10621 actaccgctg tctggcagct ggctttcact gtttaaaatc agcgtatggc ctaaatcggt
    10681 cgcgcggtta atcttgctga ttaaaaaacc agcgataacc ggagatttga tcttacccag
    10741 cagagagcca atctcttcct gatagttatt ggctgttttg agaatgtaat cttccaactg
    10801 cttataactc ttcagatgca ataatccgag aatcacatgc aatttattca taaattcgtg
    10861 ggatcgttca cgaagtgcgt cagcatagtt gaccagaccg tcgagtcgct gcatcagttt
    10921 acgtacttca gttttgtccc tgaaggttga aatggcaccg atgataacgc cattactgcg
    10981 caccggaacg gtgttgatca gtaatagtcg gtccttaatc gtaatctctt cgtcgcggcg
    11041 tggagttccg tcgcgtaaca cttccgagac atctaccacc tgtgaccacg agtggcttag
    11101 cgtcgacagt ttctcatcgt cctgcgactt acggtaattc agcaattctt gtgcggcatc
    11161 gttgatcagc gtgacctcgc cgcgatcgtc cacggcaacg acgccttctt tgatggactg
    11221 caacatggcc tggcgttgct caaacagtgt ggagatttcg tagggttcca ggccgaaaag
    11281 gatttttttc agtacgttaa ccagaatgca ggtgccgatc agtccgacca gcatgccaaa
    11341 tagtaccgac cagataatgc tccagcgact gtcattgatc tgttgggtca cgcggcttaa
    11401 ctcaaggccg atcgccacta cgccaatttg tttatgattt tcatcgtaga tgggggtaaa
    11461 tacgcgtaaa gcctgcgcca gaaaaccgcg attgatagcg acattttctt cgccattcag
    11521 cgctttaagg atgtcatcac ctttaaatgg ctgaccaata cgctgggctt caggatgcga
    11581 gtagcgaaga ctgtgcatat cggtaacgac aataaacagc agatcgttgc gtttgcgcac
    11641 ggcttccgcg atggcctgga ttccactctc ctgcggtttt ttctgcaagc cctgacggat
    11701 ttccggcgag tcggcgaggg tacgcgctac tgccagagcc ttgttagcta gcccgtctcg
    11761 cgtcatatca ctgatttgcg agaagtaaat cagatgcacc accaatagca ccgagaacag
    11821 taccgcgctg accattaaga tcacagtggt actcaatttc atcggacgtt tgcgtaacat
    11881 acggtagggc aatgaatgtc tcatcagctt ccttgtgtga caaatttctt aagcattatc
    11941 tctgatgagg cgggtaattc aaagggagta agaatgattg gctatatagg ggaagagact
    12001 ctggcaacgg aaactgccag tgctgtatga agattccggg gctatgctta tagtgaaaac
    12061 catactgatg agagagggaa ggtcatggat caggcgctac ttgacggggg ttatcgctgt
    12121 tataccggcg aaaatatcga tgtctatttt aacactgcga tatgtcagca ttctggcaat
    12181 tgcgtacgtg gcaacggcaa gttatttaat ctcaaacgaa agccgtggat catgccggat
    12241 gaagtcgacg tcaccactgt ggtgaaagtg attgatatgt gcccaagcgg cgcgctgaaa
    12301 taccgtcata aataagcgag ggtaaaatgg aaatacgcga aggccacaat aaattttaca
    12361 ttaatgacga acaaggcaag caaatcgctg aaattgtctt tgtgccgacc ggagagaatt
    12421 tagcgattat cgaacatacc gatgtcgatg aaagcctgaa aggacaaggg attggtaaac
    12481 agctggttgc gaaagtcgtg gaaaaaatgc gtcgggaaaa acgaaaaatt atcccactat
    12541 gcccatttgc gaaacacgaa tttgataaaa cacgggagta tgatgatatt cgcagttgat
    12601 gggagagtac agagtcacga tatttttcat gctctccgcg gtgtgatgca ggagagcatc
    12661 tgaagggtag ggggatgcac aaagaatggt cagagagagc gtttttttgt cccaagtcat
    12721 cccctttact gagcaaaaaa aaaagaatat ctcctatatg agaatcatca ttcggggtta
    12781 ataagttttg cgtccccaga gcgttcaata ttgataggag tcatattatg gaaggtaaaa
    12841 acaagttcaa tacttatgtt gtttcttttg attatccatc atcttattcc tcagtgttct
    12901 taaggttaag atcattgatg tatgatatga atttctcctc tatcgtggct gatgaatatg
    12961 ggataccacg acaactgaat gaaaactcct tcgcaataac gacatcgtta gccgcaagtg
    13021 aaatcgaaga tttaatcagg ctcaaatgct tagacttacc ggatattgat tttgacctca
    13081 gcattatgac agttgatgac tatttccgtc agttttacaa gtagccagga aggataagaa
    13141 gaacataaaa aatggcacta ttctctaa
//
LOCUS       DAZFJL010000126        13102 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZFJL010000126 DAZFJL010000000
VERSION     DAZFJL010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.128"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            102..437
                     /gene="yjdM"
                     /locus_tag="ACHJ8C_003684"
     CDS             102..437
                     /gene="yjdM"
                     /locus_tag="ACHJ8C_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO5553471.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            597..1040
                     /gene="yjdN"
                     /locus_tag="ACHJ8C_003685"
     CDS             597..1040
                     /gene="yjdN"
                     /locus_tag="ACHJ8C_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO5553472.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVIKQQPTQ"
     gene            1173..1961
                     /gene="phnC"
                     /locus_tag="ACHJ8C_003686"
     CDS             1173..1961
                     /gene="phnC"
                     /locus_tag="ACHJ8C_003686"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO5553473.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSVGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGCSQQFDNERFDHLYRSINRVEENAKAA"
     gene            1986..3002
                     /gene="phnD"
                     /locus_tag="ACHJ8C_003687"
     CDS             1986..3002
                     /gene="phnD"
                     /locus_tag="ACHJ8C_003687"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5553474.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLNPAYAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKDNKGLNEQDKLAKTTAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3108..3887
                     /gene="phnE"
                     /locus_tag="ACHJ8C_003688"
     CDS             3108..3887
                     /gene="phnE"
                     /locus_tag="ACHJ8C_003688"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO5553475.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVIAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            3908..4633
                     /gene="phnF"
                     /locus_tag="ACHJ8C_003689"
     CDS             3908..4633
                     /gene="phnF"
                     /locus_tag="ACHJ8C_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO5553476.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGITLRRSQTRISARRAQAKECQRLEIPNLSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4634..5086
                     /gene="phnG"
                     /locus_tag="ACHJ8C_003690"
     CDS             4634..5086
                     /gene="phnG"
                     /locus_tag="ACHJ8C_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO5553477.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNINADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQNRHFQNLSETLITPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5083..5667
                     /gene="phnH"
                     /locus_tag="ACHJ8C_003691"
     CDS             5083..5667
                     /gene="phnH"
                     /locus_tag="ACHJ8C_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO5553478.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQPELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5667..6731
                     /gene="phnI"
                     /locus_tag="ACHJ8C_003692"
     CDS             5667..6731
                     /gene="phnI"
                     /locus_tag="ACHJ8C_003692"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO5553479.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTMEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6724..7569
                     /gene="phnJ"
                     /locus_tag="ACHJ8C_003693"
     CDS             6724..7569
                     /gene="phnJ"
                     /locus_tag="ACHJ8C_003693"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO5553480.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQN
                     EAKSQ"
     gene            7566..8324
                     /gene="phnK"
                     /locus_tag="ACHJ8C_003694"
     CDS             7566..8324
                     /gene="phnK"
                     /locus_tag="ACHJ8C_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO5553481.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVMELNLAVVIVTHDLGVARLLADRLLV
                     MKQGKVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8435..9115
                     /gene="phnL"
                     /locus_tag="ACHJ8C_003695"
     CDS             8435..9115
                     /gene="phnL"
                     /locus_tag="ACHJ8C_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO5553482.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGEEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGVSS"
     gene            9112..10248
                     /gene="phnM"
                     /locus_tag="ACHJ8C_003696"
     CDS             9112..10248
                     /gene="phnM"
                     /locus_tag="ACHJ8C_003696"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO5553483.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRQPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAM
                     CRARHIALASHDDATHAHVAQSHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10248..10805
                     /gene="phnN"
                     /locus_tag="ACHJ8C_003697"
     CDS             10248..10805
                     /gene="phnN"
                     /locus_tag="ACHJ8C_003697"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO5553484.1"
                     /translation="MTGKLIWLMGPSGSGKDSLLAELRQREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLSLIHQKEKHHACL"
     gene            10792..11226
                     /gene="phnO"
                     /locus_tag="ACHJ8C_003698"
     CDS             10792..11226
                     /gene="phnO"
                     /locus_tag="ACHJ8C_003698"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO5553485.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHQAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11228..11998
                     /gene="phnP"
                     /locus_tag="ACHJ8C_003699"
     CDS             11228..11998
                     /gene="phnP"
                     /locus_tag="ACHJ8C_003699"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO5553486.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDTIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFEAGFDGMEIEVA"
     gene            complement(12026..12355)
                     /gene="yjdP"
                     /locus_tag="ACHJ8C_003700"
     CDS             complement(12026..12355)
                     /gene="yjdP"
                     /locus_tag="ACHJ8C_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO5553487.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQRQVSDDRRKQYNDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12424..12873)
                     /gene="rpiB"
                     /locus_tag="ACHJ8C_003701"
                     /gene_synonym="alsI"
     CDS             complement(12424..12873)
                     /gene="rpiB"
                     /locus_tag="ACHJ8C_003701"
                     /gene_synonym="alsI"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HFO5553488.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEADGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
BASE COUNT         2916 a         3915 c         3654 g         2617 t
ORIGIN      
        1 tacccccgac aaactcaggg attttgtgta taattgcggc ctttttcggc aatctgccgt
       61 tttttggcgc ttttgccctg ctgacttttg aggaaatcca catgtcatta ccacactgcc
      121 caaaatgcaa ctccgaatac acctacgaag ataacggcat gtacatctgc ccggaatgtg
      181 cctacgaatg gaacgacgca gaacctgcac aggaaagcga cgagctgatc gttaaagatg
      241 ctaacggcaa tctgctggct gacggcgaca gcgttaccat cattaaagat ctgaaagtga
      301 aaggtagctc ttcgatgctg aaaattggca ccaaagtgaa aaacatccgc ctggttgaag
      361 gcgaccataa catcgattgc aaaatcgacg gttttggtcc gatgaaactg aagtctgagt
      421 ttgtgaaaaa gaactgattg tattgtgatc ggtaggccgg ataaggcgtt tacgccgcat
      481 ccggcaacgg tggcgactgc ctgatgcgac gctgaccgcg tcttatcagg cttccctctc
      541 atctgtataa atttcgaact acacttaacg ggcttctctt aactgaggtc accatcatgc
      601 cgttaagtcc ctacctctct tttgccggta actgttccga cgcgattgcc tattatcaac
      661 gtacgttggg cgcggaactg ctctataaaa tcagcttcgg cgaaatgccg aaatcagcgc
      721 aggacagcgc cgaaaactgc ccttccggaa tgcaatttcc cgataccgcc atcgcgcatg
      781 ccaacgtgcg cattgccgga agcgacatca tgatgagcga tgccatgccg tcaggaaaag
      841 ccagctactc cggctttacg ctggtgctcg attctcaaca ggtggaagaa ggaaaacgct
      901 ggtttgataa ccttgccgcc aacggaaaaa tcgaaatggc ctggcaggaa acgttctggg
      961 cgcatggctt tggcaaagtc accgataaat ttggcgtacc gtggatgatt aatgtcatca
     1021 aacaacagcc aacgcaataa cccgccggga ggcccgccct cccgcactgt catcgaattc
     1081 ccgttaactc ttcatctgtt agtcactttt aattaaccaa atcgtcacaa taatccgcca
     1141 cgatggagcc acttttttag ggaggctgca tcatgcaaac gattatccgt gtcgagaagc
     1201 tcgccaaaac cttcaatcag catcaggcgc tgcacgcggt tgatctgaac attcatcatg
     1261 gtgaaatggt ggctctgctt gggccgtcgg gttccggaaa atccaccctt ttacgtcact
     1321 taagcggttt gattaccggc gataaatctg tcggtagcca tatcgagctg ctgggccgca
     1381 ctgtccagcg cgaaggccgt ctggcccgcg atatccgcaa aagccgcgcc cataccggct
     1441 acatctttca acaattcaac ctggtgaatc gcctgagcgt actggagaac gtgctgattg
     1501 gcgcgctcgg cagcacgcca ttctggcgaa cctgttttag ctacttcacc cgcgagcaga
     1561 aacagcgcgc gttacaggcg ctgacccgcg ttggcatggt gcactttgcc catcaacgcg
     1621 tttccacact ctccggcggc cagcaacagc gtgtggcgat tgcccgcgcg ctgatgcagc
     1681 aggcgaaggt cattctcgct gacgaaccca tcgcctcgct ggacccggaa tcggcacgca
     1741 tcgtgatgga caccctgcgc gacatcaacc agaacgacgg cattaccgtg gtcgtcacgc
     1801 tgcatcaggt ggattacgcc ctgcgctact gcgaacgcat cgtcgccctg cgccaggggc
     1861 acgtcttcta cgacggctgc agccaacagt ttgataacga acgttttgac catctctacc
     1921 gcagcattaa ccgcgtcgaa gagaacgcga aagctgcctg acatccccat cattgaggaa
     1981 aacgaatgaa cgctaagata attgcctcgc tggccttcac cagcatgttc agcctcagca
     2041 ccctgttaaa tccggcatac gccgaagagc aggaaaaggc gctgaatttc ggcattattt
     2101 caacggaatc acagcaaaac ctgaaaccgc aatggacgcc gttcttacag gatatggaga
     2161 agaagctggg cgtaaaggtc aacgccttct ttgccccgga ctacgcgggc attatccagg
     2221 ggatgcgctt caataaagtg gatatcgcct ggtacggcaa cctgtcggcg atggaagcgg
     2281 tggatcgcgc caacggtcag gtcttcgccc agacggtcgc ggcggatgga tcgccgggtt
     2341 actggagcgt gttgatcgtc aacaaagata gcccgatcaa caacctgaac gatctgctgg
     2401 cgaagcggaa agatctcacc ttcggcaatg gcgatcctaa ctctacctcc ggcttcctcg
     2461 tccccggtta ctacgtcttc gctaaaaaca atatctccgc cagcgacttt aagcgcaccg
     2521 tcaacgccgg gcatgaaacc aacgccctcg ccgtcgctaa caagcaagtg gatgtcgcca
     2581 ccaacaacac cgaaaacctc gacaagctga aaacctccgc gccggagaag ctgaaagaac
     2641 tgaaggtgat ctggaaatca ccgctgatcc ctggcgatcc gatcgtctgg cgcaaaaatc
     2701 tttccgaaac cactaaagac aagatctacg acttctttat gaattacggc aaaacgccgg
     2761 aagagaaagc ggtgctggag cgcctgggct gggcgccgtt ccgcgcctcc agcgacttgc
     2821 aactggtgcc gattcgccag ctcgcactgt ttaaagagat gcaaagcgtg aaggacaaca
     2881 aagggctgaa cgaacaggac aagctggcaa aaaccaccgc cattcaggcg caactggatg
     2941 atttggaccg cctgaacaac gcgttaagcg caatgagttc ggtgagtaaa gcggtgcagt
     3001 aaatcgtagg tcggataaga cgccccggcg tcgcatccga caatgtgcag acgttgatgc
     3061 cggatgcggt gcaagcacct tatccggcct acagaccgga gccaaacatg caaaccatca
     3121 ccatcgcccc acccaagcgc agctggttct cgcttctgag ctgggccgtt gttatcgccg
     3181 tgctggtcgt ctcgtggcag ggcgcggaaa tggccccgct tacgctgatt aaagatggcg
     3241 gcaacatggc gacgttcgcc gccgacttct tcccgcccga tttcagccag tggcaggatt
     3301 acctcaccga aatggccgtc acgctgcaaa tcgccgtctg gggcaccgcg ctggcggtgg
     3361 ttctctccat ccccttcggc ctgatgagcg ctgaaaacct ggtaccgtgg tgggtctacc
     3421 aacctgtacg ccgcctgatg gacgcctgcc gtgccattaa cgaaatggtc ttcgccatgc
     3481 tgttcgtggt cgccgtcggc ctcggcccgt tcgctggcgt gctggcgctg tttatccaca
     3541 ccaccggcgt actttccaaa ctgctttccg aagcggtaga agcgattgaa cccggcccgg
     3601 tggagggcat tcgcgccacc ggtgccaaca agctggaaga gatcctttac ggcgtactac
     3661 cgcaggtcat gccgctgctg atctcctact ctctctatcg cttcgaatcc aacgtccgct
     3721 cggcgaccgt cgtcggtatg gttggtgcgg gcggaattgg cgtcacgctg tgggaagcga
     3781 ttcgcggttt ccagttccag caaacctgcg ccctgatggt gctgattatc gtcacggtca
     3841 gcctgctgga tttcctctct caacggttgc gtaagcactt tatctgatta gcgaggcatt
     3901 gatatctatg cacttgtcta cacatccgac cagctaccca acacgctatc aggagatagc
     3961 cgcaaaactt gagcaggagc ttcgtcaaca ctaccgctgc ggcgactatc ttcccgccga
     4021 gcagcaactg gcggcgcgct ttgaggtgaa ccgccacacc ctgcgccgcg ccatcgacca
     4081 actggtggaa aaaggctggg tacagcgccg tcagggcgtc ggcgtactgg tgctgatgcg
     4141 cccgttcgat tacccgctca acgcccaggc gcgttttagc cagaacctgc tggaccaggg
     4201 cagccatccc accagcgaaa aattgctatc ggtgctgcgc cccgcctccg gtcacgtcgc
     4261 tgacgcgctg gggataaccg agggggagaa cgtcatccac ctgcgcaccc tgcgtcgggt
     4321 caacggcgtc gcgctctgtt taatcgacca ctactttgcg gacctcaccc tctggccgac
     4381 gctgcaacgt ttcgacagcg gttcgctgca cgattttctg cgcgagcaaa cggggattac
     4441 gctacgacgc agccagacgc ggatcagcgc ccgccgcgcc caggccaaag agtgccagcg
     4501 tcttgaaatc ccgaatctgt cgccgctgct gtgcgtgcgc acccttaacc accgtgacgg
     4561 tgaaagcagc ccggcggagt actccgtcag cctgacgcgc gccgacatga ttgaattcac
     4621 tatggagcac tgaatgcacg cagataccgc gacccgccag cactggatgt ccgtgctggc
     4681 gcacagccaa ccggctgaac tggcagcccg cctgaacgcg ctgaacatca acgccgatta
     4741 tgaggtgatc cgcgccgctg aaactggcct ggtgcagatt caggcgcgaa tgggcggcac
     4801 cggcgaacgc ttttttgctg gcgacgccac gctcacccgc gccgctgtgc gtctgactga
     4861 cggcacgctc ggctatagct gggtgctggg gcgcgacaaa cagcacgccg aacgttgtgc
     4921 gctgattgat gcgctgatgc agcaaaaccg ccactttcaa aacttatcag aaacccttat
     4981 taccccgctg gacgctgacc gtatggcacg cattgccgcg cgtcaggccg aagtgaacgc
     5041 cagccgggtc gacttcttta cgatggttcg cggagacaac gcatgaccct ggaaaccgct
     5101 tttatgcttc ccgtgcagga tgcccagcac agttttcgtc gcctgttaaa ggccatgagc
     5161 gagccgggcg tgattgtcgc cctgcatcag ctcaaacgcg gctggcagcc gctgaatatc
     5221 gccaccacca gcgtgctgct gacgctggcc gataacgaca cgccggtgtg gctttctgcg
     5281 ccattaagta acgatatcgt cagccagagc ctgcgttttc ataccaacgc gccgctggtc
     5341 aatcagccgg aactggcgac cttcgcggta acagatgagg cgatttccag cgaacagctt
     5401 aacgcccttt ccagcggcac cgctgttgcg ccagaagcgg gcgcgacgct gattttacag
     5461 gtcgtcagcc tgagcggcgg gcgcatgctg cgccttactg gcgcgggtat tgccgaagaa
     5521 cgaatgatcg ccccgcagct gccggagtgc attctgcacg aacttaccga gcgcccgcac
     5581 ccgttcccgc tcggcatcga cctgatcctg acctgcggcg agcgcctgct ggctatcccg
     5641 cgaaccactc atgtggaggt atgctgatgt acgttgccgt gaaagggggc gagaaggcga
     5701 tcgacgccgc ccacgccctg caagagagcc gacgccgggg caataccgat ttgcctgaac
     5761 tgagcgtcgc ccagattgaa cagcagctta atcttgcagt agatcgcgtg atgaccgaag
     5821 gcggcattgc cgaccgcgaa ctggcggcgc tggcgctgaa acaggccagc ggcgataacg
     5881 tcgaagcgat tttcctgcta cgcgcctacc gcaccacgct ggcgaagctg gcggtaagcg
     5941 agccgctcga caccatggag atgcgtctcg aacggcgtat ttcagcggtt tataaagata
     6001 ttcccggcgg ccagctgctt ggcccaacct acgactacac ccatcgcctg ctcgatttca
     6061 ccctgctggc aaacggcgaa gcgccgacgc tgaccacagc cgacagcgaa caacagccgt
     6121 cgccgcacgt tttcagcctg ctggcgcgtc aggggctggc gaagtttgaa gaagatagcg
     6181 gcgcacagcc ggacgacatc acccgcacgc cgccggttta cccctgctca cgctcctccc
     6241 gcctgcaaca attaatgcgc ggcgacgaag gctatttgct ggcgctggcc tactccaccc
     6301 agcgtggtta cggacgcaat catccgttcg caggcgagat ccgcagcggc tatatcgacg
     6361 tgtcgattgt gccggaagag ctgggatttg cggtgaacgt cggcgaactg ctgatgaccg
     6421 aatgtgaaat ggtcaacggt tttatcgacc cgccggatga gccgccgcac ttcacgcgcg
     6481 gctacgggct ggtgttcggc atgagcgagc gcaaagcgat ggcgatggcg ctggttgacc
     6541 gcgctctaca agccccggag tacggcgagc acgcgacagg cccggcgcag gatgaagagt
     6601 tcgtgctggc gcatgccgac aacgtcgaag ccgcaggctt tgtctcgcac cttaaactcc
     6661 cgcactacgt cgatttccag gccgaactgg agctactcaa acgtctgcaa caggagcaga
     6721 accatggcta atctgagcgg ctacaacttt gcctacctcg acgagcagac caaacgcatg
     6781 atccgccgcg ccatcttaaa agcggtggcg atccccggtt atcaggtgcc gtttggcggg
     6841 cgcgagatgc cgatgccgta cggctgggga actggtggta tccagcttac cgccagcgtg
     6901 attggcgaaa gcgatgtgct gaaggtgatt gaccagggcg cggacgacac caccaacgcc
     6961 gtgtcgattc gtaacttctt taagcgcgtg accggggtga acaccaccga gcgcacggac
     7021 gatgcgacac ttatccagac gcgtcaccgc atacccgaaa cgccgctgac cgaagatcag
     7081 atagttatct tccaggtgcc gatccccgaa ccgctgcgct ttattgagcc gcgtgagacg
     7141 gaaacgcgca ccatgcacgc gctggaggag tacggcgtga tgcaggtgaa actgtatgaa
     7201 gatatcgccc gcttcggtca tatcgccacc acctacgcct atccggtgaa ggtgaacggg
     7261 cgctacgtaa tggacccgtc gccgatcccg aaattcgata acccgaaaat ggacatgatg
     7321 cccgccctgc aactgtttgg cgcggggcgc gagaagcgca tctatgccgt gccaccgttt
     7381 acccgcgtgg aaagcctcga tttcgatgat catccgttca ccgtccagca gtgggatgaa
     7441 ccatgcgcca tctgcggatc gacccacagt tacctcgacg aagtggtgct ggatgacgcc
     7501 ggaaaccgca tgtttgtctg ctccgatacc gattattgcc gccaacagaa cgaggccaaa
     7561 agccaatgaa tcaaccgtta ctttcggtca ataacctgac ccacctttac gcgccgggca
     7621 aaggctttag cgatgtctct tttgatttat ggccggggga agtgctgggc atcgtcgggg
     7681 aatccggctc cgggaagacc acgctgctga agtcgatctc cgcccgcctg acaccacagc
     7741 aaggggaaat tcgctacgag aaccgttcgc tgtacgcgat gagcgaggcc gaccgccgtc
     7801 gcctgctgcg caccgaatgg ggtgtggtgc atcagcatcc gcttgacggc ctgcgccgcc
     7861 aggtgtcggc aggcggcaat atcggcgaac ggttgatggc aaccggagcg cgtcattacg
     7921 gcgatattcg cgccaccgcg cagaaatggc tggaagaggt ggagattccc gccaaccgga
     7981 tcgacgacct gccgaccacc ttttccggcg gcatgcagca acgtttgcag attgcccgca
     8041 acctggtgac gcatccgaag ctggtgttta tggatgaacc cactggcggg ctggatgtgt
     8101 cggtacaggc gcgcctgctg gacctgctcc gcggtctggt gatggagctt aacctggcgg
     8161 tagtgattgt cacccacgat ttaggcgtcg cccgtctgct ggcggaccgt ttgctggtaa
     8221 tgaagcaagg gaaagtggtg gagagtgggt taaccgaccg ggtgctcgac gacccgcacc
     8281 atccgtatac ccagttgctg gtgtcatcgg ttttgcagaa ttgagctgat gccggatgcg
     8341 gcgtaaacgc cttatccagc ctacaaatgc gctccccgta ggtcggataa gacgcatcag
     8401 cgtcgcatcc gacacccgaa ccacgaggcg aaaaatgatt aacgtacaaa acgtcagtaa
     8461 aaccttcatc ctgcaccagc aaaacggcgt gcgcctgccc gtcttacaac gtgcctcgct
     8521 caccgtcaac gccggcgaat gcgtggtgct tcacggccac tccggcagcg gaaaatcaac
     8581 cctgctgcgc tcgctgtacg ccaactatct gcccgacgaa gggcaaatcc agatcaaaca
     8641 cggtgaggaa tgggtagacc tggtcaccgc gccagcccgc aaagtggtgg agatccgcaa
     8701 aaccaccatc ggctgggtga gccagtttct gcgcgtcatc ccgcgtattt cggcactgga
     8761 agtggtgatg cagccgctgc tcgataccgg cgtaccgcgt gaagcctgcg ccgctaaagc
     8821 cgcccgatta ctgacgcgcc tgaacgtgcc tgaacgcctg tggcacctgg caccatcgac
     8881 cttctccggc ggcgaacagc agcgcgtcaa catcgcccgt ggctttatcg tcgactatcc
     8941 gattctgctg ctggatgaac ccaccgcctc gctggacgcc aaaaacagcg ccgcggtggt
     9001 ggaattaatt cgcgaagcca aagcccgtgg cgcggccatc gtcggtatct tccatgacga
     9061 agccgtacgc aatgacgtcg ccgaccgcct gcacccaatg ggagtctctt catgattatc
     9121 aataacgtca aactggtgct ggaaaacgaa gtggtgcacg gttcactgga agtacaagat
     9181 ggcgaaattc gcgcctttgc cgaaagccag agccgccagc cggaagcgat ggacggcgaa
     9241 ggtggctggc tgctgccggg gctgattgag ctgcataccg ataatctgga taaattcttc
     9301 accccgcgtc caaaagtgga ctggcccgcc cactcggcaa tgagtagcca cgacgcgctg
     9361 atggtggcga gcggcatcac caccgtgctg gacgcggtgg cgattggcga cgtgcgcgac
     9421 ggcggcgatc ggctggagaa tctggagaag atgatcaacg ccatcgaaga gacgcagaaa
     9481 cgcggcgtca accgcgccga gcaccgtctg catctgcgct gcgaactgcc gcatcacacc
     9541 acgctgccgc tgtttgaaaa actggtgcag cgcgaaccgg tgacgctggt gtcgctgatg
     9601 gatcactcgc caggccagcg ccagttcgcc aaccgcgaga agtatcgcga gtattatcag
     9661 ggcaaatact cgctcaccga tgcgcagatg caacagtacg aagaggagca actggcgctc
     9721 gccgcacgct ggtcacagcc gaatcgcgaa tccatcgccg caatgtgccg cgcgcgacac
     9781 attgcgctcg ccagccacga tgacgccacg cacgcccacg tagcccaatc ccatcagctt
     9841 ggcagcgtga tcgccgaatt tcctaccaca ttcgaagcgg cagaagcctc gcgcaagcac
     9901 ggcatgaacg tactgatggg cgcgccgaat atcgtgcgcg gcggttcgca ctccggcaac
     9961 gtggcggcca gtgaactggc gcagcttggc ctgctggata tcctctcttc cgactactac
    10021 cccgccagcc tgctggatgc ggcgtttcgc gtggccgatg accagagcaa ccgctttacg
    10081 ctgccgcagg cggtgaggct ggtgaccaaa aacccggccc aggcactgaa tcttcaggat
    10141 cgcggggtga ttggcgaggg caaacgcgcc gacctggtgc tggcgcatcg caagggcaat
    10201 cacattcata ttgaccacgt ctggcgtcag ggtaaaaggg tgttctgatg acgggaaaac
    10261 tgatttggtt aatgggtccg tccggctccg ggaaagacag cctgctggcg gaactccgcc
    10321 agcgggaaca aacccagcta ctggtagcgc atcgctacat cacgcgtgat gccagcgccg
    10381 gaagtgaaaa ccatatcgcc ctgagcgagc aggagttttt tacccgcgcg gggcagaacc
    10441 ttctggcctt aagctggcac gctaacggtc tgtattatgg cgtcggcgtc gagattgacc
    10501 tctggctgca cgctggattt gacgtggtgg tcaacggctc acgcgcccat ctgccgcagg
    10561 cgcgggcgcg ctatcaatcg gcgctgctgc ccgtttgttt acaggtttcg ccagagatcc
    10621 tgcgccagcg cctggaaaac cgtggtcgtg aaaatgccag tgaaattaac gcccggctgg
    10681 cgcgcgccgc ccgctatact cctcaggatt gccatacgct caataatgac ggcagcctgc
    10741 gccagtcggt cgacacgctg ctgtcgctga ttcatcagaa ggagaaacac catgcctgct
    10801 tgtgaacttc gcccggcaac gcagtacgac accgacgcgg tttacgcgct gatttgtgag
    10861 ctaaaacaag cggagtttga ccaccaggcg tttcgcgtgg gctttaacgc caacctgcgt
    10921 gacccgaata tgcgctacca tctggcgctg ttggatggcg aggttgtcgg catgatcggc
    10981 ctgcatttgc agtttcatct gcatcatgtc aactggatcg gcgaaattca ggaactggtg
    11041 gtgatgccgc aggcgcgcgg tctgaatgtc ggcagtaaat tactggcatg ggcagaagaa
    11101 gaagcccgcc aggccggggc cgaaatgacc gagctttcca ccaacgtgaa gcgccacgac
    11161 gcgcaccgat tttacctgcg cgaaggctac gagcagagcc acttccgctt caccaaggcg
    11221 ctgtaacatg agcctgaccc tcacgctcac cggcaccggc ggcgcacagg gcgttccggc
    11281 atggggctgc gagtgcgcgg cctgcgccag agcgcgacgc tcgccgcagt atcgccgcca
    11341 gccatgcagc ggcgtcgtga agtttaacga cgcgatcacc ctgatcgacg ccgggctgca
    11401 cgatctcgcc gatcgctggt cgcccggatc gttccagcag tttttgctga cgcactacca
    11461 tatggatcac gtccaggggc tgttcccgct gcgctggggc gtgggcgaca caatcccggt
    11521 ctacggtcca ccggatgagc agggctgcga cgatctgttt aaacatccgg gcctgcttga
    11581 tttcagccac acggtggagc cgtttgtggt gtttgatttg caggggttgc aggtcacgcc
    11641 gctgccgctt aaccattcga aactcacttt cggctattta ctggaaacgg cgcacagccg
    11701 ggtggcgtgg ctatctgaca ccgcaggcct gccggaaaaa acgctgaaat ttttacgcaa
    11761 taatcagccg caggtaatgg tgattgattg cagccacccg ccgcgcgagg atgcaccgcg
    11821 taatcactgt gatttaaata ccgtgcttgc gctgaatcag gttatccgct cgccgcgggt
    11881 tattctgacc catatcagcc atcagtttga tgcgtggctg atggaaaacg cgctaccatc
    11941 ggggtttgag gcggggtttg aggcggggtt tgatgggatg gagattgagg tggcgtgagg
    12001 gaaaataccc gcgccggccc ctatatcaac gcccatactc atcttccatt ctccgctctt
    12061 catcttccag ttgccgacgc tcctgatcaa gctggcgctg gcgatcgtcc agctgcctgc
    12121 ggcgatcttc aaactggcgg cggcgatcgt tatattgctt gcgacgatcg tcgctcacct
    12181 ggcgttgcca accgtcgtcg cgcgaatctt catagtctcg cccacggtca gggttataag
    12241 cgtcgttaat cgcctgctga atattgccaa tggtgtcgtc gataatatcg gcctgggccg
    12301 gaacggtgga cagcgtgagc agggtgaata aaagaaatag cggaaagcgt ttcattagcc
    12361 aacctcaaaa agaaactcta tccacattag tcattactca tccatgcaag tagtggatga
    12421 atctcaattt ctccgctgct ctattgccgt aatcgcctcc acgcgttgtt gatgacgacc
    12481 gccttcgtac tgtgcgccca gccacgcatc gacaatcatt tttgccagtt cgaggccaac
    12541 tactcgtgaa ccaaaagcca gcacgttggt gtcgttatgc tgccgcgaaa gttgcgcgga
    12601 ataaggttcg ctacaaacga ccgcgcgaat tccggcaaac ttgttcgccg ctatcgaaat
    12661 accgacgcca gtgccacaaa tcaaaatccc gccatcagcc tctccgccag caacggccag
    12721 tgcgacttga ctggcgtaat gcggataatc agtacgctct gacgaccagg ttcctttatc
    12781 aatcacttca acgccacgct caactaaatg tgccactatt tcatgtttta aaatgaaacc
    12841 gacatgatca cagccaaatg caatcttttt cattcatctt ccccgcccgt gcaaaacttc
    12901 acaatcaaaa aacatttgtg atgtaaatga attaaaaaca cccaaaatcg atgaaatccg
    12961 ccttcccgat ctgttttaca gagttacatc tggcagcgca atagcgttaa agacactcac
    13021 tatccagcaa catctatcat ctaataaacc aggaaaacaa ataacatcat gtttttaaag
    13081 tgattaaatg aaattaaatt tt
//
LOCUS       DAZFJL010000127        13055 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFJL010000127 DAZFJL010000000
VERSION     DAZFJL010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.129"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..1099)
                     /locus_tag="ACHJ8C_003702"
     CDS             complement(<1..1099)
                     /locus_tag="ACHJ8C_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5553489.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     AGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFATIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEVDFETAMWTIPGEREPLEGVKHSHRG
                     SKMRTTHIVPLSRQALAILEKIKGLSGNRELIFVGDHDPHKPMSEDTVNKALRVMGYD
                     TKTEVCGHGFRTMACSSLIESGLWSRDAVERQ"
     gene            complement(1298..1382)
                     /locus_tag="ACHJ8C_003703"
     tRNA            complement(1298..1382)
                     /locus_tag="ACHJ8C_003703"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1346..1348),aa:Leu,seq:caa)
     gene            1578..2597
                     /gene="ahr"
                     /locus_tag="ACHJ8C_003704"
     CDS             1578..2597
                     /gene="ahr"
                     /locus_tag="ACHJ8C_003704"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO5553490.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2727..4229
                     /gene="yjgR"
                     /locus_tag="ACHJ8C_003705"
     CDS             2727..4229
                     /gene="yjgR"
                     /locus_tag="ACHJ8C_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO5553491.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTTSEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPTFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASIEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(4390..5472)
                     /gene="lptG"
                     /locus_tag="ACHJ8C_003706"
     CDS             complement(4390..5472)
                     /gene="lptG"
                     /locus_tag="ACHJ8C_003706"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO5553492.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5472..6572)
                     /gene="lptF"
                     /locus_tag="ACHJ8C_003707"
     CDS             complement(5472..6572)
                     /gene="lptF"
                     /locus_tag="ACHJ8C_003707"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO5553493.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6635..6685)
                     /gene="ytgA"
                     /locus_tag="ACHJ8C_003708"
     CDS             complement(6635..6685)
                     /gene="ytgA"
                     /locus_tag="ACHJ8C_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO5553494.1"
                     /translation="MSIIILATISTTQENK"
     gene            6839..8350
                     /gene="pepA"
                     /locus_tag="ACHJ8C_003709"
     CDS             6839..8350
                     /gene="pepA"
                     /locus_tag="ACHJ8C_003709"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO5553495.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8608..9051
                     /gene="holC"
                     /locus_tag="ACHJ8C_003710"
     CDS             8608..9051
                     /gene="holC"
                     /locus_tag="ACHJ8C_003710"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO5553496.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9051..11906
                     /gene="valS"
                     /locus_tag="ACHJ8C_003711"
     CDS             9051..11906
                     /gene="valS"
                     /locus_tag="ACHJ8C_003711"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO5553497.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRASAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIVDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11953..>13055)
                     /locus_tag="ACHJ8C_003712"
     CDS             complement(11953..>13055)
                     /locus_tag="ACHJ8C_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFO5553498.1"
                     /translation="VNFLLTIITLGIYGPWALIKCRRYIYQHVTLKGQPFSYKGTGGA
                     IFVSMLLIVVVYLLSISCFAGQHFALGLFLFALLICGIPCMAVKSLQYQANMTSLNDI
                     RFGFNCSMMRAWWVMLGLPVLLALVFWFALYLIAQVTTSIGGLFFNLVALSLLSAIGL
                     GVVHGITYSKWMPLLGNNATFGIHKFSIQVNVKECIKGCMLAILTMVPFIIVIGIMIA
                     PVFQQLMMMTMLGRSDAGSEFVLQYYPQIMASYFLYFVAILVFASYLYVTLRNLFLNN
                     LTLANGTIRFHSSVTAIGMLLRMLAVLMGSSITCGLAYPWLKMWMVSWIANNTHVQGD
                     LDSLELTNDDKPQDSGSLMWISRGIMPYVPFI"
BASE COUNT         3305 a         3369 c         3467 g         2914 t
ORIGIN      
        1 tctgccgttc cactgcatcc ctcgaccaca atcctgattc aatcagcgaa ctacacgcca
       61 tcgttctgaa accatgtcca cagacttccg ttttcgtgtc atagcccatc actcgtaaag
      121 ccttattcac cgtgtcctca ctcataggct tatgcggatc gtgatcaccc acaaagatca
      181 gctctcgatt cccactcaag cctttgatct tttccagaat agccagcgcc tgacgagaca
      241 aaggcacaat atgagtcgtc cgcatcttcg aaccacgatg agaatgctta acgccttcca
      301 gcggttcacg ctcaccagga atcgtccaca tagccgtttc aaaatctact tcagaccagc
      361 gagcaaaacg cagttcgctt gaacggatga aaactagtaa cgtgagttcg acagccagtc
      421 gggttaacgg tctgccagag tagtgatcga tgcggtgaag taattcagga atgcggttaa
      481 gttccaatgc agcacgatgc tgtcttttcg ccgtagcaac cgcaccggca atttcttgcg
      541 cggggttata atcgattagg ccgctctgca cagcaaagcg cataatcgcg gtagtgcgct
      601 gctgtaaacg ggcggcgact tcaagtcgcc cggatgactc gacagcttta atgggtacaa
      661 gaagatcccg agtcttaagc tccgcaatgt tccgcttacc gatggtagca aagagattat
      721 cttcaagact tttcaataca cgagcgctat gcgatgccga ccatttctga ttactggcgt
      781 gccagtctct ggcaaccact tcaaacgtta tcgcctcttg ttcctgctct actttaacgg
      841 ctttcttgtt ctcacttgga tcgacaccat tagctaacag cttacgggct tcatcccggc
      901 gtgcccgggc atccgccaac gacacttcag ggtatttccc cagcgccagc atcttttctt
      961 ttccagcaaa gcgataacgt agccgccaat atttagaacc attagggtga accagcaaaa
     1021 ccatgccttc accgtcagta agtttatagg ctttagcttc aggctttgcc gaacgaacct
     1081 tcacatcact cagagccatg atgagtatcc tttcaagggt tctgtgcggg tacaaacatt
     1141 atcgaaccgg gatatacccg cagatgtacc cacatcagta agttgatgta gattgaatca
     1201 ggttgactta ggttgagtga aaaagctgga aaagccttgc gtagcgcgga tttcaggcat
     1261 aaaaaaagac ctcaattgag gtctatttac atacttttgg tgccgaaggc cggactcgaa
     1321 ccggcacgta tttctacggt tgattttgaa tcaactgcgt ctaccgattt cgccacttcg
     1381 gcactgaaga ggtatgcgga aaacgttgtg aattatacct gtcacgcgcc accatgcaag
     1441 cgccagcttg ctcgcacctc gctaagtgct gaaattttca gcattgcata ccgcgatgtg
     1501 taacctttgt cacactccag gcaccccgcc ctgccatgct ctacacttct cagaccacac
     1561 cagagaagga ccaaaaaatg tcgatgataa aaagctatgc cgcaaaagaa gcgggtggcg
     1621 aactggaagt ttatgagtac gatcccggtg aactgaagcc acaagatgtt gaagtgcagg
     1681 tggattactg cgggatctgt cattccgatc tgtcaatgat cgataacgaa tggggatttt
     1741 cacaatatcc actggttgcc gggcatgagg tgattggtcg cgtagtggcg ctcgggagtg
     1801 ccgcgcagga taaaggtttg caggtcggtc agcgtgtcgg gattggctgg acggcacgca
     1861 gctgcgggca ttgcgatgcc tgtattagcg gtaatcagat caactgcgag caaggtgcgg
     1921 tgccgacgat tatgaatcgc ggtggctttg ccgagaagtt gcgtgcggac tggcaatggg
     1981 tgattccact gccagaaaat atcgacattg aatctgccgg accgctgttg tgcggcggta
     2041 tcacggtctt taaaccgctg ttgatgcacc atatcactgc caccagccgc gttggggtaa
     2101 ttggtattgg cgggctgggg catatagcta taaaacttct gcacgcaatg ggatgcgagg
     2161 tgacagcctt tagttctaat ccggcgaaag agcaggaagt gctggcgatg ggtgccgata
     2221 aagtggtgaa tagccgcgat ccgcaggcac tgaaaactct ggcggggcag tttgatctca
     2281 ttatcaacac cgtcaacgtc agcctcgact ggcagcctta ttttgaggcg ctgacctatg
     2341 gcggtaattt ccatacggtc ggtgcggtcc tcacgccgct gtctgttccg gcctttacgt
     2401 taattgcggg cgaccgcagc gtctctggtt cagcaaccgg tacaccttat gagttgcgta
     2461 agctgatgcg ctttgccgcc cgcagcaagg ttgcgccgac taccgaactg ttcccgatgt
     2521 cgaaaattaa cgacgccatc cagcatgtgc gcgacggtaa ggcacgttac cgagtggtcc
     2581 tgaaagccga cttctgatcc ctttgccggg tgcctgtcgc ccggcgttta aatctctttg
     2641 gaaggcgtct cgcaatctgc tgaatttagc ggtgaagagt gcgcagccgt tgcctatact
     2701 ccaggcagga aactggagga aatctcatga gcgaacccct gttaattgcc cgcacgccgg
     2761 acacagaact gtttttactg ccgggaatgg ctaaccgtca cgggctgatt actggcgcaa
     2821 cggggacggg taaaactgtc acgctgcaaa aactggcaga gtcattgtcg gaaatcggtg
     2881 ttccggtgtt tatggccgat gtgaaaggcg atctgaccgg catcgcgcag gcaggaacga
     2941 cctcggaaaa actgctcgca aggcttaaaa atatcggtgt caatgactgg caaccgcatg
     3001 ccaatccggt ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg cgggcgacgg
     3061 tttcggatct ggggccgctg ttgctggcac gactgttgaa tctcaacgat gtgcaatctg
     3121 gcgtgctgaa tatcatcttc cgcattgctg acgatcaggg attgttgctg ctcgacttta
     3181 aagatctgcg ggcaattacc cagtacatcg gcgataacgc caaatccttc cagaatcagt
     3241 acggtaatat cagtagtgca tcggttggtg ccatccagcg cgggctgttg tcgctggaac
     3301 agcaaggcgc agcacacttc tttggtgagc cgatgctgga tatcaaagac tggatgcgca
     3361 ccgatgccaa cggtaaaggc gttatcaata tccttagcgc cgaaaaactc taccagatgc
     3421 cgaaactgta cgccgccagc ctgttgtgga tgctttcaga gttgtatgaa caattgccgg
     3481 aagcgggcga tctggagaaa ccaaaactgg tgtttttctt cgacgaagcg catctgctgt
     3541 ttaacgatgc accgcaggta ctgctggata agattgagca ggtgataagg cttattcgct
     3601 caaaaggcgt gggcgtctgg ttcgtttcgc aaaacccgtc agatatcccg gataacgtgc
     3661 tcgggcagct cggtaatcgc gttcaacacg ctttgcgtgc ttttacgcct aaagatcaga
     3721 aagcagtgaa ggcagcggcg caaaccatgc gggccaatcc gacgtttgat accgaaaagg
     3781 caatccagga actggggacg ggcgaagcgt taatctcgtt tctcgatgcg aaaggaagtc
     3841 cttctgtggt ggaacgggcg atggtgattg cgccttgttc gcggatgggg ccggtgacgg
     3901 aagatgagcg caatggcttg attaaccact ccccggtgta tggcaagtac gaggatgacg
     3961 tggaccggga atccgcctat gagatgttgc aaaaaggctt tcaggccagt atcgagcagc
     4021 aaaataatcc tcccgcgaaa gggaaagagg tggcggtgga tgacggtatt ctcggtggat
     4081 tgaaggatat tttgtttggc actaccggac cacgcggcgg gaagaaagat ggtgtggtgc
     4141 aaacaatggc aaaaagcgcc gcccgccagg tgacgaatca gattgtgcgc gggatgttgg
     4201 ggagtttgct gggggggaga agaaggtaag ccaggtgcga tttgtctttg cctgatgcga
     4261 cgctggcgcg ccttatcatg cccacaccac tgcaatatat tgaattttca tgatttttca
     4321 ggcatgaacg aagcacactt tgctaacaat aataaaggga gctttcgctc cctttattcg
     4381 ttcatcaggt tacgattttc tcattaacag ccacaggctg attaagaaga agctggcgct
     4441 tggcaacagt gcgccgatga ttggcgggat gccgtaaacc aacgtcagcg ggccgaagat
     4501 ctggtccagt acgtagaaga caaaaccgaa gctaatacca gtgaccacac gcacgcccat
     4561 cggtacgcta cgcagtgggc caaagatgaa cgacagcgcc atcagcatca tcaccgccac
     4621 ggatagcggc tggaagattt tgctccacat gttgagctga taacgtccgg catcctgacc
     4681 gctcgacttc agatacttca catagttgtg caaaccgctg atagagagtg catccgggtc
     4741 cagcgccacc acgcccagtt tgtctggcgt gaggttggtt ttccaggtgc cgctcaccgt
     4801 ctgcgaaccg gtgatctgtt tcggattggt caaatcagat tcatcaacct gcgacagacg
     4861 ccaaacttta tgttccgggt caaacttcgc ggtagcggca tagcgtacgg attgcagacg
     4921 acgattctcg ttaaaggcat aaatactgat gccacctaac tcttcgtcac ctttaacccg
     4981 ctcaatgtag acgaagttgt tgccatcttt cgcccataaa ccttgctggg tagagagcaa
     5041 cgagccgcca tacatcgcct gcgcacggta gttacgcgcc atctgctcgc cctgcggcgc
     5101 gacccattca ccaatcgcca tcgtcagcaa gaccagcgga atggcggttt tcatcaccga
     5161 cagcgccacc tgcatacggg taaaaccaga agcctgcatc accaccagtt cgctgcgctg
     5221 cgccagcatc ccaagaccaa gcaacgcccc aagcagagcc gccatcggga agaagatttg
     5281 cacatctttc ggcacgctca gcaaggtata cattcctgcg cctaacgcgt cgtaactccc
     5341 ctgcccggct tttttcagct gatcgacaaa cttgataatg ccggaaagcg acaccagcat
     5401 gaacagcgtc atcatgatgg tggtgaaaat agttttaccg atatagcggt caagtacgcc
     5461 aaaaggttgc atcacaccgc tcctttacgc gaaaaactgg cgcgcaggcg gcggaccggc
     5521 acggtgtccc aaaggttgag aacaatcgcc aaagccagat aaatcaggtt aacggtccac
     5581 atccacagcg tcgggtccag cttaccttta ccgccgttcg atttcaggga ggtctggatc
     5641 aggaagaaca acagatacag cagcatggct ggcagcatcg atagtacgcg cccctgacgc
     5701 gggttaacca cgctcagcgg tacgaccata agtgccatca taaacacggt gaacaccaac
     5761 gtgatacgcc agttcagttc tgcgcgagca cgatcggtgt cggtgttcca caatgtgcgc
     5821 atgtccatct ggtcggtatc gttcgggtcg agcgccaccg cctggtgacc aatgatcgcc
     5881 tgataatcct ggaaatccgt aatgcggaaa tcacgtaaca gtgcagtgcc ttcgaagcgc
     5941 gttccctggt tgagagtgac gacctgggag ccgtcgcgca gctgggttaa atgtccggaa
     6001 tcggccacca ccacagaagg tcgtgcatta ccttttggtc gaatttgcgc gaggaacaca
     6061 tctttgaaat cgctgccgtc aacgctttcg atgaacagca ccgagctgcc attagtcgct
     6121 tgctggaatt gcccttgcgc cagcgccgcc atgccagggt tcgctttcgc ttctgctaac
     6181 acttcatcct gatgacgcga tgaccatggt cccgcccaca tcacgttaac cgccgcgacg
     6241 attgccgtga acaccgcaag gatcattgcc gctttcacca gcaccgcttt gctcagaccg
     6301 caggcatgca ttaccgtaat ttcactttcg gtatacagtt tgcccagcgt catcagcagc
     6361 ccgaggaaca ggcttaatgg caggataagc tgcgccattt ccggcacgcc caacccgaga
     6421 agggagagca ccaggttcgc cggaatatcg ccgtcaaccg ctgcgccgag gatcctcact
     6481 aacttttgac agaagaagat caaaagcaag atgaagagta tcgccagctg gcttttgagc
     6541 gtctcccgca ccagatatct tatgattatc actttaaata cgcccgtaaa aactcgtctt
     6601 ttgcaggatt ttagcttgtt tcatggctta aacgtcattt attctcttga gtcgtcgaaa
     6661 tcgtcgctaa gataattata ctcaacggat tcacctctca gattttgttc tgacgtgcca
     6721 atgccgtaat aacgttaaga ttaacacgaa gtcatcgcaa cagcggacat gagttacgaa
     6781 agcttgcaat tctatctgta gccaccgccg ttgtctttaa gattcaggag cgtagtgcat
     6841 ggagtttagt gtaaaaagcg gtagcccgga gaaacagcgg agtgcctgca tcgtcgtggg
     6901 cgtcttcgaa ccacgtcgcc tttctccgat tgcagaacag ctcgataaaa tcagcgatgg
     6961 gtacatcagc gccctgctac gtcggggcga actggaagga aaaccggggc agacattgtt
     7021 gctgcaccat gtgccgaatg tactttccga gcgaattctc cttattggtt gcggcaaaga
     7081 acgtgagctg gatgagcgtc agtacaagca ggttattcag aaaaccatta atacgctgaa
     7141 tgatactggc tcaatggaag cggtctgctt tctgactgaa ctgcacgtta aaggccgtaa
     7201 caactactgg aaagtgcgtc aggctgtcga gacggcaaaa gagacgctct acagtttcga
     7261 tcagttgaaa acgaacaaga gcgaaccgcg tcgtccgctg cgtaaaatgg tgttcaacgt
     7321 gccgacccgc cgtgagctga ccagcggtga acgcgcgatc cagcatggtc tggcgattgc
     7381 cgcagggatt aaagccgcga aagatctcgg caatatgcca ccgaatatct gtaacgctgc
     7441 ttacctcgcc tctcaggcgc gccagttggc tgacagctac agcaagaatg tcatcacccg
     7501 agttatcggc gaacagcaga tgaaagagct ggggatgcat tcctatctgg cggtcggtca
     7561 gggttcgcaa aacgaatcgc tgatgtcagt gattgagtac aaaggcaacg cgtcggaaga
     7621 tgcacgccca attgtgctgg tgggtaaagg tttaaccttc gactccggcg gtatctcgat
     7681 caagccttca gaaggcatgg atgagatgaa gtacgatatg tgcggtgcgg cagcggttta
     7741 cggtgtgatg cggatggtcg cggagctgca actgccgatt aacgttatcg gcgtgctggc
     7801 aggctgcgaa aacatgcctg gcgggcgtgc ctatcgtccg ggcgatgtgt taaccaccat
     7861 gtccggtcaa accgttgaag tgctgaatac cgacgctgaa ggccgcctgg tactgtgcga
     7921 cgtattaact tacgttgagc gttttgagcc ggaagcggtg attgatgtgg cgacgctgac
     7981 cggtgcctgc gtgatcgcgc tgggtcatca tattactggt ctgatggcga accacaatcc
     8041 gctggcccat gagctgattg ctgcatctga acaatccggt gaccgcgcat ggcgtttacc
     8101 gctgggtgac gagtatcagg aacagctgga gtccaatttt gccgatatgg cgaacattgg
     8161 cggtcgtcct ggtggggcga ttaccgcagg ttgcttcctg tcgcgcttta cccgtaagta
     8221 taactgggcg cacctggata tcgccggtac tgcctggcgt tctggtaagg cgaaaggcgc
     8281 aaccggtcgc ccggtggcgt tgctggcaca gttcctgcta aaccgcgccg ggtttaacgg
     8341 cgaagagtaa ttgtatcagg ctgtgttgtt attgtcggat gcggcgtgaa cgccttatcc
     8401 gacctacaca gcactgaact cgtaggcctg ataagacgcg acagcgtcgc atcaggcaag
     8461 gctgttattg tcggatgcga cgtgaacgcc ttatccgacc tacacacagc acttaacttg
     8521 taggcctgat aagacgcggc agcgtcgcat caggcgctgc ggtgtatacc tgatgcgtat
     8581 ttaaatccac cacaagaagc cccatatatg aaaaacgcaa cgttctacct tctggacaat
     8641 gacaccaccg tcgatggctt aagcgccgtt gagcagttgg tgtgtgaaat tgccgcagaa
     8701 cgttggcgca gtggtaagcg cgtactcatc gcctgtgaag atgaaaagca ggcttaccga
     8761 ctggatgaag ccctgtgggc gcgtccggca gaaagctttg ttccgcataa tttagcggga
     8821 gaaggaccgc gcggcggtgc gccggtggag atcgcctggc cgcaaaagcg cagcagcagc
     8881 ccgcgcgata tattgattag tctgcgaaca agctttgcag attttgccac cgctttcaca
     8941 gaagtggtag acttcgttcc ttatgaagat tctctgaaac aactggcgcg cgaacgctat
     9001 aaagcctacc gcgtggctgg tttcaacctg aatacggcaa cctggaaata atggaaaaga
     9061 catataaccc acaagatatc gaacagccgc tttacgagca ctgggaaaag cagggctact
     9121 ttaagcctaa tggcgatgaa agccaggaaa gtttctgcat catgatcccg ccgccgaacg
     9181 tcaccggcag tttgcatatg ggtcacgcct tccagcaaac catcatggat accatgatcc
     9241 gctatcagcg catgcagggc aaaaacaccc tgtggcaggt cggtactgac cacgccggga
     9301 tcgctaccca gatggtcgtt gagcgcaaga ttgccgcaga agaaggtaaa acccgtcacg
     9361 actacggccg cgaagctttc atcgacaaaa tctgggaatg gaaagcggaa tccggcggca
     9421 ccattacccg tcagatgcgc cgtctcggca actccgtcga ctgggagcgt gaacgcttca
     9481 ccatggacga aggcctgtcc aatgcggtga aagaagtttt cgttcgtctg tataaagaag
     9541 acctgattta ccgtggcaaa cgcctggtaa actgggaccc gaaactgcgc accgctatct
     9601 ccgacctgga agtggaaaac cgcgaatcga aaggttcgat gtggcacatc cgctatccgc
     9661 tggctgacgg cgcgaaaacc gcagacggta aagattatct ggtggttgcg actacccgtc
     9721 cggaaaccct gctgggcgat actggcgtgg ccgttaaccc ggaagatccg cgttataaag
     9781 atctgattgg caaatatgtc attctgccgc tggttaaccg tcgtattccg atcgttggcg
     9841 acgaacacgc cgacatggaa aaaggcaccg gctgcgtgaa gatcacccca gcgcacgact
     9901 ttaacgacta tgaagtcggt aaacgtcatg ccctgccgat gatcaacatc ctgacctttg
     9961 acggcgatat ccgtgcaagc gcccaggtgt tcgataccaa aggtaacgaa tctgacgttt
    10021 attctagcga aatcccggca gagttccaga aactggagcg ttttgctgca cgtaaagcag
    10081 tcgttgctgc ggttgacgcg ctcggcctgc tggaagaaat taaaccgcac gacctgaccg
    10141 ttccttatgg cgaccgtggt ggcgtggtta tcgaaccgat gctgaccgac cagtggtacg
    10201 tgcgtgccga tgtactggcg aaaccggcag ttgaagcggt tgagaatggc gacattcagt
    10261 tcgtaccgaa gcagtacgaa aacatgtatt tctcctggat gcgcgatatt caggactggt
    10321 gtatctctcg tcagctatgg tggggtcacc gtatcccggc atggtatgac gaagcgggta
    10381 atgtttatgt tggccgcaac gaagacgaag tgcgtaaaga aaataacctc ggtgctgatg
    10441 ttgcgctgcg tcaggatgaa gacgttctcg acacctggtt ctcctccgcg ctgtggactt
    10501 tctctacact cggctggccg gaaaacaccg acgcactgcg ccagttccac ccaaccagcg
    10561 tgatggtatc tggttttgac atcatcttct tctggattgc ccgcatgatc atgatgacca
    10621 tgcacttcat caaagatgaa aatggcaaac cgcaggtgcc gttccacacc gtttacatga
    10681 ccggcctgat tcgtgatgac gaaggccaga agatgtccaa atccaaaggt aacgtgattg
    10741 acccactgga tatggttgac ggtatttcgc tgccggaact gctggaaaaa cgtaccggca
    10801 atatgatgca gccgcagctg gcggacaaaa tccgtaagcg caccgagaag cagttcccga
    10861 acggcattga gccgcacggt actgacgcgc tgcgcttcac cctggcggcg ctggcttcta
    10921 ccggtcgtga catcaactgg gatatgaagc gtctggaagg ttaccgtaac ttctgtaaca
    10981 agctgtggaa cgccagccgc tttgtgctga tgaatacgga aggtcaggat tgcggcttta
    11041 acggcggtga aatgacgctg tcgctggcgg atcgctggat tctggcggag ttcaaccaga
    11101 ccatcaaagc gtaccgcgaa gcgctggaca gcttccgctt cgatatcgcc gcaggcattc
    11161 tgtatgaatt cacctggaac cagttctgtg actggtatct ggagctgacc aagccggtaa
    11221 tgaacggtgg aacagaagcc gaactgcgcg gtactcgcca tacgctggtg actgtactgg
    11281 aaggtctgct gcgcctcgcg catccgatca ttccgttcat caccgaaacc atctggcagc
    11341 gtgtgaaagt actttgcggt atcactgccg acaccatcat gctgcagccg ttcccgcagt
    11401 acgatgcgtc tcaggttgat gaagcggcac tggccgacac cgagtggctg aaacaggcga
    11461 tcgttgctgt acgtaacatc cgtgcagaaa tgaacatcgc gccgggtaaa ccgctggagc
    11521 tgctgctgcg tggttgcagc gcggatgcag aacgtcgcgt aaatgaaaac cgcggcttcc
    11581 tgcaaacgct ggcgcgtctg gaaagcatca ccgtgctgcc tgctgatgat aaaggtccgg
    11641 tttccgttac aaagatcgtc gacggcgcag aactgctgat cccgatggca ggcctcatca
    11701 acaaagaaga tgagctggca cgtctggcga aagaagtagc gaagatcgaa ggcgaaatca
    11761 gccgtattga gaacaagctg gcgaacgaag gctttgtcgc ccgcgcaccg gaagcggtca
    11821 tcgcgaaaga gcgtgagaag ctggaaggct acgcagaagc gaaagcgaag ctgattgaac
    11881 agcaggctgt tatcgccgcg ctgtaatact catagtgaaa acgaaggccg gagcatgctc
    11941 cggccttttt atttagataa acggaacata cggcataata ccgcgagata tccacattaa
    12001 cgaaccgcta tcctgcggtt tgtcatcatt ggttaactcc agcgaatcaa ggtcgccctg
    12061 gacatgggta ttgttagcaa tccagctaac catccacatt ttcagccagg gataagccag
    12121 accacaggta attgaggacc ccattaatac cgccaacatg cgcagaagca taccgatcgc
    12181 ggtaacagat gaatgaaaac gaattgtccc atttgccagc gtcagattat tcaaaaacag
    12241 attgcgtaac gtgacataga gataactggc aaaaacaagg atcgcgacaa aatacaggaa
    12301 ataagaagcc ataatctgcg ggtaatattg taatacaaac tcactgccag catcagacct
    12361 acccaacatg gtcatcatca ttaactgctg gaatactggt gcgatcataa taccaatcac
    12421 aatgatgaaa ggaaccatgg ttaatattgc gagcatgcaa cctttaatgc attcttttac
    12481 gtttacctga atagaaaact tatgaatgcc aaaagttgca ttattaccca gcaatggcat
    12541 ccatttacta taggttatac catggacaac acctagcccg atggcagaaa gcaaacttaa
    12601 tgcgactaag ttaaagaata accctcctat tgatgtcgtc acttgtgcaa taagatacag
    12661 tgcgaaccaa aaaactaacg ccaacagaac aggcagtcct agcataaccc accatgcacg
    12721 catcattgag caattaaagc caaagcgaat gtcattcagt gaggtcatgt tcgcctgata
    12781 ctgcagactc ttcactgcca tacaaggaat accgcagatc aagagagcaa acaggaataa
    12841 accgagcgca aagtgttgac ctgcaaaaca agaaatactg agaagataaa ccaccacaat
    12901 aagtaacatg ctaacaaaaa tggcaccacc tgttccctta taactaaatg gttgcccctt
    12961 gagtgtcaca tgttgataaa tataacgacg gcattttatc aatgcccacg gcccatatat
    13021 tcccagagta ataattgtca gtaaaaaatt cacaa
//
LOCUS       DAZFJL010000128        12776 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZFJL010000128 DAZFJL010000000
VERSION     DAZFJL010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.130"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            40..591
                     /locus_tag="ACHJ8C_003713"
     CDS             40..591
                     /locus_tag="ACHJ8C_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFO5553499.1"
                     /translation="MQNIAVVYYSGYGHTKLVAEKVAQGAHADLISIDNEGNITEQEW
                     EKLDAADAIIFGAPTYMGGAPWQFKKFADASSKAWFTRKWQDKVFGGFTNSASLNGDK
                     QVTLIYLQTLASQHGGLWVSLGQAPANVLASTREDVNNLGGSVGLLVQSPADAGADQI
                     PTGDLDTAVKYGERVATITVRLK"
     gene            complement(769..1761)
                     /gene="rpoS"
                     /locus_tag="ACHJ8C_003714"
     CDS             complement(769..1761)
                     /gene="rpoS"
                     /locus_tag="ACHJ8C_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO5553500.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(1824..2918)
                     /gene="nlpD"
                     /locus_tag="ACHJ8C_003715"
     CDS             complement(1824..2918)
                     /gene="nlpD"
                     /locus_tag="ACHJ8C_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO5553501.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(3103..3729)
                     /gene="pcm"
                     /locus_tag="ACHJ8C_003716"
     CDS             complement(3103..3729)
                     /gene="pcm"
                     /locus_tag="ACHJ8C_003716"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO5553502.1"
                     /translation="MVSRRVQALLDQLRAQGIQDELVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(3723..4484)
                     /gene="surE"
                     /locus_tag="ACHJ8C_003717"
     CDS             complement(3723..4484)
                     /gene="surE"
                     /locus_tag="ACHJ8C_003717"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO5553503.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(4465..5514)
                     /gene="truD"
                     /locus_tag="ACHJ8C_003718"
     CDS             complement(4465..5514)
                     /gene="truD"
                     /locus_tag="ACHJ8C_003718"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO5553504.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(5511..5990)
                     /gene="ispF"
                     /locus_tag="ACHJ8C_003719"
     CDS             complement(5511..5990)
                     /gene="ispF"
                     /locus_tag="ACHJ8C_003719"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO5553505.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYALGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(5990..6700)
                     /gene="ispD"
                     /locus_tag="ACHJ8C_003720"
     CDS             complement(5990..6700)
                     /gene="ispD"
                     /locus_tag="ACHJ8C_003720"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO5553506.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLVNHPRITVVDGGEERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(6719..7030)
                     /gene="ftsB"
                     /locus_tag="ACHJ8C_003721"
     CDS             complement(6719..7030)
                     /gene="ftsB"
                     /locus_tag="ACHJ8C_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO5553507.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNNDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(7223..7546)
                     /locus_tag="ACHJ8C_003722"
     CDS             complement(7223..7546)
                     /locus_tag="ACHJ8C_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO5553508.1"
                     /translation="MRNSHNITLTNNDSLTEVEDTTWSLPGAVVGFLSWLCALAIPML
                     IYGSNTLLFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLATVGIMFGALF
                     MWLLG"
     gene            complement(7596..8201)
                     /gene="cysC"
                     /locus_tag="ACHJ8C_003723"
     CDS             complement(7596..8201)
                     /gene="cysC"
                     /locus_tag="ACHJ8C_003723"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO5553509.1"
                     /translation="MALHDENVVWHSHPVTPQQREQHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(8201..9628)
                     /gene="cysN"
                     /locus_tag="ACHJ8C_003724"
     CDS             complement(8201..9628)
                     /gene="cysN"
                     /locus_tag="ACHJ8C_003724"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO5553510.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(9630..10538)
                     /gene="cysD"
                     /locus_tag="ACHJ8C_003725"
     CDS             complement(9630..10538)
                     /gene="cysD"
                     /locus_tag="ACHJ8C_003725"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO5553511.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRINLQSGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            10790..11827
                     /gene="iap"
                     /locus_tag="ACHJ8C_003726"
     CDS             10790..11827
                     /gene="iap"
                     /locus_tag="ACHJ8C_003726"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO5553512.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANIQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     NTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGITVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(11952..12104)
                     /locus_tag="ACHJ8C_003727"
     CDS             complement(11952..12104)
                     /locus_tag="ACHJ8C_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5553513.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            12166..>12776
                     /locus_tag="ACHJ8C_003728"
     CDS             12166..>12776
                     /locus_tag="ACHJ8C_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5553514.1"
                     /translation="MQMQHLMVGYPKYYQTADYALRLSVMADIATMRMKALHFWDKHG
                     ISAASEAFGVSCRTLYWWRQLLNKEGPEGLIPHSKAPLVRRKKHWHPDVLKEIRRLRT
                     ELPNLGKEQIFVRLKPWCEQRYLACPSVSTIGRMIAAAHDKMRMIPVRLGSRGKALLV
                     KKRSAKPRRPKHYRPVKTGELIGMDAIELRMGELRRYVITMID"
BASE COUNT         3078 a         3461 c         3166 g         3071 t
ORIGIN      
        1 tgcctcaaaa aatcattcat tagtaaaaag gatatttata tgcaaaacat agctgtggtt
       61 tattattcag gatatggtca cactaaactg gttgctgaaa aagtggcaca aggcgcccat
      121 gcagatttaa tttcgattga taacgaagga aatatcactg agcaggagtg ggaaaaactg
      181 gacgcagccg atgccattat cttcggtgct cccacctata tgggcggtgc gccatggcaa
      241 ttcaagaaat ttgctgatgc cagttccaaa gcgtggttta cacgtaaatg gcaggataaa
      301 gtcttcgggg gtttcaccaa cagcgccagc ctgaatggcg ataaacaagt tacgctgatc
      361 tatctgcaaa cgctggcttc acagcatggc ggtctgtggg tgagtttagg tcaggctcct
      421 gccaatgtgc ttgcgtcaac cagggaagat gtaaacaatc tgggcggttc agtcggtttg
      481 ctggtgcaat ctccagccga tgcgggtgca gaccaaatcc ctacgggcga tctggataca
      541 gccgtgaaat atggtgaacg cgtggcgaca attactgtgc gcttaaaatg atgattgcca
      601 aatgtgacgc tgacgcgtct atccggtctt ctacatctaa aaagtgccgt cccctggtca
      661 gtcagataaa gttttaacgc tgtacccgat aacagttcaa catgtaccat gtaccaaaag
      721 aaaaaaagcc agcctcgctt gagactggcc tttctgacag atgcttactt actcgcggaa
      781 cagcgcttcg atattcagcc cctgcgtttg caggatttcg cgcaaacggc gcaggccttc
      841 aacctgaatc tggcgaacac gttcacgggt gaggccaatt tcacgaccta catcttccag
      901 tgttgccgct tcgtacccca gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc
      961 gttcagctcg aacagccatt tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc
     1021 ttccggacca ttctcttttt catcggccag gatgtccagc aacgcttttt cggaatcacc
     1081 acccagcggg gtgtctaccg aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc
     1141 atcaactggc ttatccagtt gctctgcgat ctcttccgca cttggttcgt ggtccagctt
     1201 atgggacaac tcacgtgcgg ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg
     1261 caaacgaata gtacgggttt ggttcataat cgcccgttcg atcgtctggc gaatccacca
     1321 ggttgcgtat gttgagaagc ggaaaccacg ttccgggtca aacttctcta ccgcgcggat
     1381 cagccccagg ttgccctctt cgatcaggtc cagcaacgcc agaccacgat tgccataacg
     1441 gcgggcaatt tttaccacca gacgcaagtt actctcgatc atccggcggc gagaggcgac
     1501 atctccacgc agtgcgcgac gcgcaaaata aacttcttct tcggccgtta acagtggtga
     1561 ataaccaatc tcaccaaggt aaagctgagt cgcgtccagc acacgctgtg tggctccctg
     1621 cgataacagt tcctcttcgg ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt
     1681 ttcgtcaaaa acctcaactc cgttctcatc aaattccgca tcttcattta aatcatgaac
     1741 tttcagcgta ttctgactca taaggtggct cctacccgtg atcccttgac ggaacattca
     1801 agcaaaagcc tggttccgcc gatttatcgc tgcggcaaat aacgcagcgg gtttacggat
     1861 ttccccttgt aacgaatttc aaaatgcaag cgtgttgaac tggttccggt gctacccatg
     1921 gttgctattt tttgccccgc cttcacttct tgttgttccc ggaccagcat tgtgtcgtta
     1981 tgggcgtagg cactcaggta atcatcatta tgtttgatga taatcagatt accgtagccg
     2041 cgcagcgcgt taccggcata aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct
     2101 ttgctgcctg cgatatcaat ccccttgttg cccccctcag aagcgccaaa ggtttcgatc
     2161 actttgccct cagtcggcca gcgccaggtg gagataggcg tactggttga tgtactgctg
     2221 acagtcggct cggttgtgct tgctgttggt accgttacag gcgctgtgac cgtggtcgca
     2281 gttggcttgt tgttcggcaa cattttgtta gcactctgtt cacccgaaga ctcagaatac
     2341 gtaattgtcg gttgcgacgc aacagcaacg gtggaatttt gtgcaggctt gatcacaact
     2401 ccttgctctg ctgcgtcggc ctgggtaatg gcatttccgc ctgtgattgg cgtaccggaa
     2461 gcattaccca cctgtaaggt ctgaccgacg ttcagcgcgt atggtgcctg aatattgttg
     2521 cgctgagcaa ggtcacggaa atcgttgcca gtaatccagg cgatataaaa aagcgtgtcg
     2581 ccttttttca cggtgtatgt actgccgcta taactgcctt tcggaatgtt cccatactga
     2641 cggttgtaga cgattcgtcc gttttccatc tgtaccggct gctgagctac tggctgcaca
     2701 ggctggattt gcggttgttg agtagcctga atttgtggct gctgtaccgg ctgaatttgc
     2761 ggttgctgcg ctgtagacgt cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa
     2821 ttggtatttg cgggcgcatt gccattaacg gagctgaccg gtgccggtgg atttgaagtg
     2881 tcagaacagc ctgccagcca tagcgaaacc agtgacaatg ccgcaatgcg gcgaacggtg
     2941 aattttgggc ttcccgcgct catttatccc ccaggaaaaa ttggttaata accagtgaca
     3001 taattaccgt gcaaggcacc atactgaaca ctggaaaaga tgttcacgat acgctgactt
     3061 gcggcaaaat aaccaggaaa aatccaggta tttcctcacg ttttaagcca gctcaccctt
     3121 cactaaaggg acaaagcgca cggcctccac ggtatcgata ataaattcgc ctccccgacg
     3181 acgcacccgt tttaaatact ggtgctcctc ccctacgggt aagacgagaa tcccgccttc
     3241 gtccagctgc gtcattagcg cagttggaat ttcaggcggt gccgccgtaa caatgatagc
     3301 gtcaaacggc gcgcgtgcct gccaaccttg ccatccatcg ccatgacggg ttgaaacatt
     3361 atgtaaatca agatttttca ggcggcgacg cgcctgccac tgcaagcctt taatccgttc
     3421 aaccgagcaa acatgttgga caagatgcgc caggattgca gtttgatatc ccgaaccagt
     3481 gccaatttcc agcacccgcg actgcggcgt cagctcgagt aattcggtca ttcgcgccac
     3541 catatacggc tgcgaaattg tctgcccctg acctatcggc aaggcgatat tgtcccaggc
     3601 tttttgttca aacgcttcat caacgaattt ttcacgcggc acggcggcaa gtgcattcag
     3661 caccagctca tcctgaatac cttgcgcacg taattgatcc agaagtgctt gtacgcgtct
     3721 gcttaccatt gcgtgccaac tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg
     3781 ctatgcgcag ttaaatccac atgcagcggc gtgatggaaa catagccctc atctaccgca
     3841 gcaaaatcgg tccccggacc ggcatcacat ttaccgcccg gcgggccaat ccagtacagc
     3901 gtattgccgc gcggatcttg ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag
     3961 cgcgtcacgc gaataccttt gatttgatcc aagggtaaat ccggaacgtt aatattaaga
     4021 atacgcccgg tgcgcagcgg ctctttacac agtgcgcgca aaattgagca ggttaccgcc
     4081 gcggcagtgt cgtaatgttt atgcccgtca agcgagacgg caagcgccgg aaaacctaaa
     4141 tgacggcctt ccatcgcggc cgctaccgta ccggaataaa taacatcatc ccccagattc
     4201 ggcccggcgt taattccgga cacaacaatg tccgggcgcg ggcgcatcag agcatttacg
     4261 ccaagataga cgcaatcggt cggggttccc atttgcacag caatatcacc attttcaaag
     4321 gtaaacgtgc gcagggagga ttccagtgtc agagaattag aagcgccgct gcggttacga
     4381 tcgggggcga ccacctgaac gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata
     4441 ccgggtgcat gtaccccgtc atcattactc agcaatatgc gcataatcac ctgttgtgtt
     4501 gataagttcc ctgacaacgc tggttgcaaa acttcccgcc ggaagccaga aacggatttc
     4561 tacggtgacg tcatcccacc agttccagct taattgttgc ggatacagca gcatcgctct
     4621 gcgcgcggct tcaacttttt cgcgcaccag taaggcttgt aattcagttt ctgcggcgac
     4681 agctgcttgt tcgaatgcca acgcttcacg ctgagttccc cattcgccac tgcccggcaa
     4741 cgcagcggtt atcatcagct ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc
     4801 ggtggttgcg acaaaccagc taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac
     4861 ttgattaacg tctgcttttt tgaggcgctc agcaacaatc tgattaaaca acgcactgcg
     4921 ggctgccgac aaccaaaaac tccgtttatt gcgatcgcgc accggagtat tggtttgcgc
     4981 ccagcgcagc gcaccctgca agttgctacc gccaatccca aaacgttggg caccgaagta
     5041 gttcggtaca ccttttacgc aaatatcgat cagacgttgt tcaacgtcat cgcgattgct
     5101 cacttcgcgt agtaccaggg taaaggcgtt acctttcagc gcccctaaac gcagcttgcg
     5161 cttgtgccgc gcatactcca gcacctggca gccttccagt tgaaaggcgc tcagatcggg
     5221 catctctttg cccggcacgc gagcgcataa ccactgttcc gtaacagcat gtttatcttt
     5281 ttgcccagcg aagctgactt cacgggcatg aattttcagg aatttcgcca gtgcatccgc
     5341 cacaaaacgg gtattgcagc cgtttttgag gattctaacc agaatatgct caccttcacc
     5401 atcaggctca aagcccaaat cttccaccac cacaaagtct tccggattgg ctttcagcag
     5461 cccggtgcct tgcggtttac cgtggaggta agtgagatta tcaaactcaa tcattttgtt
     5521 gccttaatga gtagcgccac cgcttcacag gcaatccctt ccccacgtcc ggtaaatccc
     5581 agtttttccg tagtagtggc tttcacgtta acatcatcca tatggcagcc gagatcttcg
     5641 gcaataaaca cgcgcatttg tggaatgtgc ggcaacatct tcggtgcctg agcgatgata
     5701 gtgacatcga cgttgccgag ggcataacct ttcgcctgaa tacgacgcca ggcttcgcgc
     5761 aacagctcgc ggctgtcggc acctttaaat gccggatcgg tatccgggaa cagcttgcct
     5821 atatctccca gcgccgccgc gccaagcaat gcatcggtca acgcatggag cgccacgtcg
     5881 ccatcagaat gcgccagcag ccctttttcg taaggaatgc gtacgccacc aatgataatt
     5941 gggccttcac cgccaaaggc gtgtacgtca aaaccgtgtc caattcgcat tatgtattct
     6001 cctgatggat ggttcgggtg aggtaaaatt cggccagcgc caaatcctcc gggcgcgtta
     6061 ccttgatgtt atccgcgcgg ccttcaacaa gttgcggatg aaaaccgcaa tattccagcg
     6121 ccgaggcctc atcggtaatg gtagcgcctt catttagagc gcgcgtcagg cagtcatgta
     6181 acagctcacg agggaaaaat tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg
     6241 tatgagcaat ggcatttttg cccggttcgg cacgtttcat cgtatcgcgc accggcgcag
     6301 cgaggatccc tcccgtgcgg ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct
     6361 gatgcagaca aggacgagcg gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt
     6421 tcagacctgc cagcacggaa tcggcacgct cttcaccacc atcaacaacc gtgattcgtg
     6481 gatgattcac cagaggaagt tgtgcaaagc ggctatcgcc aggacttatg gcaatgacga
     6541 cacgtttcac cctgggatgc gccagcagcg catgcaccga gtgttcaaga atggtttgat
     6601 taccgattga gagatattgc ttaggacatt ccgtttgcat tcggcggcca aatccggccg
     6661 ccggaaccac ggcgcaaaca tccaaatgag tggttgccat gttaattccc gggctgattt
     6721 atcgattgtt ttgccccgca gactgtgcgc gctttgacgc gtcaggcacc agacgataaa
     6781 acgtttcgcc cggcctggtc atgctgagtt cattacgcgc acgctcttcg agcgcctcct
     6841 ggccgccatt gagatcgtca atttcggcaa aaagctgatc gtttcgcgct ttaagtttcg
     6901 cgtttgtagc ttgctgtgcc gccacatcat tattgacgcg agtatagtca tgtataccgt
     6961 tcttaccgaa ccacagcgaa tactgtagcc agaccagaat agccagcaac agcagcgtta
     7021 gtttacccat cctgccccct gaaaaacggc atcttcatcc catgcatccg aagacgactc
     7081 tacatcctct gttggggata ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt
     7141 tacgtatacc cagggcgtgc agaacataat ctcattatta gttacggatt gaattatgaa
     7201 cagaatagag aggaaagaac aattaaccca gtagccacat aaacagcgca ccaaacataa
     7261 tgccaaccgt cgccagagtg aaaacaatac tgtagcgtag ctttccgtcc atcaatgaat
     7321 gcagtgcaat ccccaccacc actgcgacgg gcatcagcgc cagaaagaaa ggccaggtgt
     7381 agataaaaaa gagcagcgtg ttagagccat aaatcaacat cggaattgcc aacgcgcaca
     7441 gccacgataa aaaaccaacc acagcgcctg gcagcgacca tgtggtgtct tcaacctcag
     7501 taaggctgtc gttatttgtt aatgtaatgt tgtggctgtt acgcatatct gatcctgtta
     7561 ctttgacgaa ccgggcatgg aaacccggcg gtgtctcagg atctgataat atcgttctgt
     7621 ctcaacagat ctaataattg ctgtaccaaa tttgttacta attgttcacc attgagatga
     7681 atttctgccg attcaggcgc ttcgtaaacg gaatctattc ccgtaaagtt gcgcagttca
     7741 ccggcacgcg ctttcttata taaacctttt ggatcgcggg cttcgcaaat cgccagcggc
     7801 gtatcgacaa acacttcgat aaagcgccct tctcctacgc gttcgcgaac catctggcgt
     7861 tcggcgcggt gtggcgagat aaatgcggtc agcaccacca gtccggcttc aaccatcaaa
     7921 ttcgccactt caccgacgcg acggatattc tctttacggt cggcatcgct aaaaccgaga
     7981 tcgctgcata atccgtggcg aacgttgtcg ccatccagca gatacgtact gacgccaagt
     8041 ttatgtaatg cctcctccag cgcccccgcg acggttgatt taccggaacc agagaggccg
     8101 gtaaaccaca gcacaacacc acgatgaccg tggtgctgtt cgcgttgttg cggagttact
     8161 gggtggctat gccagacgac gttttcgtca tgcagcgcca ttatttatct cccagtaaat
     8221 cgcgcgcacc ccaatgcggg aaatggcggc gaaccagggc attcaattcc agttcgaatg
     8281 cactgaattc agatggcgca gcagttgcct ggctaactgg ttcgtgcacc ataccggcac
     8341 ctacggtcac attactcagg cgatcgataa aaatcagccc accggtaacc gggttttgct
     8401 gataacgatc taacaccagt ggctcgtcaa aggtgagatc taccaggccg atgccgttca
     8461 gcggcaggtt ttcaacttcg cgttgggtaa ggttattgat atcaacctga taacgaatgc
     8521 catcaacacg agcacgcgtc ttcttaccgg caattttgat gtcgtaactc tggcccgggg
     8581 aaagcggctg ttccgccatc cataccacat ccaccgacgc gctctgcaca gccggtaacg
     8641 cttcgtctgc cgccagcagc agatcgccac ggctgatgtc gatctcatcc gtcagcacca
     8701 gggtgatcgc ttctccggca aaggcttctt cgcgatcgcc atcaaaagtc acgatccgcg
     8761 cgacgtttga ttccacacca gagggcagca ctttgacacg ttgcccgact tccacgcgac
     8821 cggatgccag cgttccggcg tagccacgaa aatcgagatt cggacggtta acgtactgca
     8881 ccgggaagcg cattggctga gcatccacca ctcgctggat ctccacggtt tccagcactt
     8941 ctagcagtgt cggaccgctg taccacggca tactttcact ttgcgaagcc acgttgtcgc
     9001 cttccagtgc ggagagcggc acaaagcgga tatccagatt acccggcagc tgcccggcaa
     9061 aagtcagata atcttcacga atacgggtga acgtctcttc actgtaatcc accagatcca
     9121 ttttgttgat cgccacgacc agatgtttga tccccaacag tgtggagata aaactgtgac
     9181 gacgggtttg atcgagcacg cctttacggg catcgatcag taagatcgcc agttcacatg
     9241 tcgatgcgcc agtcgccata ttgcgggtgt actgctcgtg ccctggggtg tcggcgataa
     9301 taaatttacg cttctcggta gagaaatagc ggtaggccac atcaatagtg atgccctgtt
     9361 cgcgctcagc ttgcaggccg tccaccagca gtgccagatc cagcttttcg ccctgggtgc
     9421 cgtgacgctt actgtcgtta tgcagcgatg agagctgatc ttcgtagatt tggcgggtat
     9481 cgtgcagcag gcgaccaatc agggtacttt tgccgtcatc gacgctacca caggtcagaa
     9541 aacgcagcag gcttttatgt tgttgcgcaa tcatccaggc ttcgacgccg ccttcattgg
     9601 cgatttgttg tgcaagtgcg gtgttcatct taaaaatacc cctgacgttt tttcagctcc
     9661 atagaccccg cctggtcgcg gtcaattacg cggccctgac gttcactggt ggtggaaacc
     9721 agcatctctt cgatgatctc cggcagtgtt tgcgcatttg actccaccgc gccggtcagc
     9781 ggccaacagc ccagtgtgcg gaaacgcacc atccgttttt taatcacttc gcccgattgc
     9841 aggttgatac ggttgtcatc aatcatcatc agcataccgt cgcgttccag aaccggacgt
     9901 tccgcagcga gatacagcgg aacaatgtcg atattttcca gccagatgta ttgccagata
     9961 tcctgctcgg tccagttaga gagcgggaag acgcggatgc tttcgccttt gttaatttgc
    10021 ccgttgtagt tgtgccacag ctccgggcgc tgatttttcg gatcccagcg atggaagcgg
    10081 tcacggaaag agtagattcg ctctttagcg cgggatttct cttcgtcacg gcgcgcacca
    10141 ccgaaggcgg catcaaaacc gtatttgttc agcgcctgtt tcaggccttc ggttttcata
    10201 atatcggtat gtttcgcgct gccgtgtacg aatggattaa tccccatcgc cacgccttcc
    10261 gggtttttat gcaccagcag ctcgcagccg taggctttcg ccgtacgatc gcggaactca
    10321 tacatctcgc ggaatttcca gccggtatcg acatgcagca acgggaaagg cagcgtacct
    10381 ggataaaacg ccttgcgcgc cagatgcagc atgacgctgg aatctttacc gatagagtag
    10441 agcatcaccg gatttgagaa ttctgcagcc acctcgcgaa taatgtggat gctttccgcc
    10501 tccagttgcc gcaggtgggt aagtcgtttt tgatccataa ccgttccttt gcaataccgc
    10561 tattttcttg ccatcagatg tttcgactat agggagcgta agagaacgaa tgaaattacc
    10621 aattagaatg agtagttcct taacggaata acgatttggc aaagctaata tcaaaaagtg
    10681 cttaaggcac cggatttcgg gcgtttagga agatttgaaa ttgttttagc gcagcggcag
    10741 tttcatacta tggcggtaaa aaaatttgca tggtatttaa ggactcacta tgttttccgc
    10801 attgcgccac cgtaccgctg ccctggcgct cggcgtatgc tttattctcc ccgtacacgc
    10861 ctcgtcacct aaacctggcg attttgctaa tatccaggca cgacatattg ctactttctt
    10921 tccgggacgc atgaccggaa ctcctgcaga aatgttatct gccgattata ttcgccaaca
    10981 gtttcagcaa atgggttatc gcagtgatat tcggacattt aatagtcggt atatttatac
    11041 cgcccgcgat aatcgcaaga gctggcataa cgtgacggga agtacggtga ttgccgctca
    11101 tgaaggcaaa gcgccgcagc agatcatcat tatggcgcat ctggatactt acgccccgct
    11161 gagcgatgct gacgccgatg ccaatctcgg cgggctaacg ttacaaggaa tggatgataa
    11221 cgccgcaggt ttaggtgtca tgctggaact ggctgaacgc ctgaaaaata cgcctaccga
    11281 gtatggtatt cgatttgtgg cgaccagcgg cgaagaggaa ggaaaattag gcgctgagaa
    11341 tttactcaag cggatgagtg acaccgaaaa gaaaaatacg ctgctggtga ttaatctcga
    11401 taacttaatt gttggcgata agctgtattt caacagtggc gtaaacacac ctgaagcagt
    11461 aaggaaatta acgcgcgacc gggcgctggc aattgcccgc agccatggaa ttgccgccac
    11521 cacaaatcca ggtttgaata aaaactatcc gaaaggcacc gggtgttgta atgacgcaga
    11581 aatattcgac aaagccggta ttactgtact ttcggtggaa gcgactaact ggaatcttgg
    11641 caataaggat ggttatcagc aacgcgcaaa aacagccgca ttccctgcgg gaaatagctg
    11701 gcatgacgta agactggata atcaacagca tattgataag gcacttcctg gaagaataga
    11761 acgtcgctgc cgtgacgtta tgcggataat gctacctctg gtgaaagagt tggcgaaggc
    11821 atcttaatgg gtttgaaatg attactccct tgcctgcagg aaatcaatgc caccagaaac
    11881 agagtataaa aaagggtgca ggagaccaaa caatcacaat tcagtacgcg gggttttacc
    11941 ccgcgtggtg gctacttctt cgattcgtaa gcgagaactg ccttaaactc cataccacgt
    12001 tctctgatac tcaactcaca caacgagtca cctaccatca acgtgaatcc cagtactgta
    12061 caacacagta cgatgattgc cacaagggca tattttgtca gcatgtctgt gtaagttcac
    12121 agagatattg caattgcctc cggataagta aggggagatt gcactatgca aatgcagcat
    12181 ctgatggttg gctatcctaa gtactaccaa acggccgatt atgcgttgag gctttcagtg
    12241 atggctgata tagcaacaat gagaatgaag gctctgcact tctgggataa acacggtatt
    12301 tctgcagctt ctgaagcctt tggcgtgtcc tgccgcacgc tttactggtg gcgtcagtta
    12361 ctgaacaagg aaggacctga ggggctaatc ccgcacagta aggcacctct ggtgcgacga
    12421 aaaaaacact ggcatcccga tgtgctgaaa gagattcgac gcctcaggac agagctgccg
    12481 aacctcggta aagagcagat ttttgttcgc ctgaagccct ggtgcgaaca gcgctatctg
    12541 gcctgtccga gtgtttccac catcggcaga atgatcgctg ccgcacatga taaaatgcgg
    12601 atgatacctg tgcgtctggg atcgcggggg aaggcactgc ttgtcaaaaa gcggtctgcc
    12661 aaaccccgca ggccaaagca ctaccgcccg gtaaagacag gtgagctcat tgggatggac
    12721 gccattgagc tcagaatggg cgaactgcgt cgctatgtca tcaccatgat cgacga
//
LOCUS       DAZFJL010000129        12758 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFJL010000129 DAZFJL010000000
VERSION     DAZFJL010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.131"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            169..1227
                     /gene="citC"
                     /locus_tag="ACHJ8C_003729"
     CDS             169..1227
                     /gene="citC"
                     /locus_tag="ACHJ8C_003729"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO5553515.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1242..1538
                     /gene="citD"
                     /locus_tag="ACHJ8C_003730"
     CDS             1242..1538
                     /gene="citD"
                     /locus_tag="ACHJ8C_003730"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO5553516.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1535..2443
                     /gene="citE"
                     /locus_tag="ACHJ8C_003731"
     CDS             1535..2443
                     /gene="citE"
                     /locus_tag="ACHJ8C_003731"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO5553517.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2454..3986
                     /gene="citF"
                     /locus_tag="ACHJ8C_003732"
     CDS             2454..3986
                     /gene="citF"
                     /locus_tag="ACHJ8C_003732"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO5553518.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRALLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYSGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGAAVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3990..4541
                     /gene="citX"
                     /locus_tag="ACHJ8C_003733"
     CDS             3990..4541
                     /gene="citX"
                     /locus_tag="ACHJ8C_003733"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO5553519.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGDILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4516..5394
                     /gene="citG"
                     /locus_tag="ACHJ8C_003734"
     CDS             4516..5394
                     /gene="citG"
                     /locus_tag="ACHJ8C_003734"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO5553520.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLNGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5445..6908
                     /gene="citT"
                     /locus_tag="ACHJ8C_003735"
     CDS             5445..6908
                     /gene="citT"
                     /locus_tag="ACHJ8C_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO5553521.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7022..7828
                     /gene="rna"
                     /locus_tag="ACHJ8C_003736"
     CDS             7022..7828
                     /gene="rna"
                     /locus_tag="ACHJ8C_003736"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO5553522.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGRTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQSNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8058..8468
                     /gene="rnk"
                     /locus_tag="ACHJ8C_003737"
     CDS             8058..8468
                     /gene="rnk"
                     /locus_tag="ACHJ8C_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO5553523.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8611..8700
                     /gene="yldA"
                     /locus_tag="ACHJ8C_003738"
     CDS             8611..8700
                     /gene="yldA"
                     /locus_tag="ACHJ8C_003738"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO5553524.1"
                     /translation="MSEALGITAFYIFIAAIIVAAVLYLEQRW"
     gene            8824..9252
                     /gene="uspG"
                     /locus_tag="ACHJ8C_003739"
     CDS             8824..9252
                     /gene="uspG"
                     /locus_tag="ACHJ8C_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO5553525.1"
                     /translation="MYKTIIMPVDVFEMELSDKAIRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(9372..10937)
                     /gene="ahpF"
                     /locus_tag="ACHJ8C_003740"
     CDS             complement(9372..10937)
                     /gene="ahpF"
                     /locus_tag="ACHJ8C_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO5553526.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLIGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(11108..11671)
                     /gene="ahpC"
                     /locus_tag="ACHJ8C_003741"
     CDS             complement(11108..11671)
                     /gene="ahpC"
                     /locus_tag="ACHJ8C_003741"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO5553527.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            12043..>12758
                     /gene="dsbG"
                     /locus_tag="ACHJ8C_003742"
     CDS             12043..>12758
                     /gene="dsbG"
                     /locus_tag="ACHJ8C_003742"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO5553528.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLSVAANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQ"
BASE COUNT         3003 a         3372 c         3247 g         3136 t
ORIGIN      
        1 caaaatttca cgccgaaata ctccttagga tgtatagcga aaggagaaaa agatatacct
       61 cgatcgcccc ctttctccca agtgaaaata aaaggttatc aatttgcaac attgaacaac
      121 attcgttgca aatcgataac aacatgcacc ttcaggatac tatttattat gttcggcaat
      181 gatattttca cccgcgtaaa acgttcagaa aataaaaaaa tggcggaaat cgcccaattc
      241 ctgcatgaaa atgatttgag cgttgacacc acagtcgaag tatttattac cgtaacccgc
      301 gatgaaaagc ttatcgcgtg cggtggaatt gccggaaata ttattaaatg cgttgctatc
      361 agtgaatccg ttcgcggtga aggactggcg ctgacattag ccactgaact gataaacctc
      421 gcctatgagc ggcatagcac gcatctgttt atttatacca aaaccgaata cgaggcgctg
      481 ttccgccagt gcggtttttc cacgctgacc agcgtacccg gcgtgatggt gctgatggaa
      541 aacagcgcca cgcgactgaa acgctatgcc gaatcgctga aaaaatttcg tcatccaggg
      601 aacaagattg gctgcattgt gatgaacgcc aatcccttta cgaatggtca ccgttatctg
      661 attcaacagg ctgcggcaca gtgcgactgg ttgcatctgt ttttagtcaa agaagattct
      721 tcacgcttcc cctatgaaga ccggctggat ctggtgttaa aaggcaccgc cgatattcca
      781 cgcctgaccg tgcatcgcgg ctccgaatac atcatctccc gcgctacgtt cccttgctac
      841 ttcattaaag aacagagcgt cattaaccat tgttacaccg aaattgatct gaagattttc
      901 cgtcagtacc tcgctcccgc gctgggtgta actcaccgct ttgtcggtac tgaacccttt
      961 tgtcgcgtta ccgcccagta caaccaggat atgcgctact ggctggaaac gccgactatc
     1021 tccgcaccgc ccatcgaact ggttgaaatt gagcggctgc gttaccagga gatgccgata
     1081 tccgcttccc gggtacgtca actgctggcg aaaaacgatc tcacggctat cgcgccgctg
     1141 gtccctgcag tcacgctgca ttatttgcag aacctgcttg agcactcccg ccaggacgcg
     1201 gcagctcgtc aaaagacccc cgcatgagaa acaggtgaaa aatgaaaata aaccagcccg
     1261 ccgttgcagg cacccttgag tctggggatg tgatgatacg catcgcccca ctcgatacgc
     1321 aggatatcga cctgcaaatc aacagcagcg ttgagaaaca gtttggcgat gcaattcgca
     1381 ccaccattct ggacgttctc gcccgctaca acgtgcgcgg cgtacagctg aatgtcgatg
     1441 acaaaggcgc actggactgc attttacgtg cacgactgga agccctgctg gcacgcgcca
     1501 gcggtatccc ggctctgcca tgggaggatt gccaatgatt tccgcttcgc tgcaacaacg
     1561 taaaactcgc acccgccgca gtatgttgtt tgtgcctggt gctaatgccg cgatggtcag
     1621 caactccttt atctacccgg ctgatgccct gatgtttgac ctcgaagact ccgttgcatt
     1681 gcgtgaaaaa gacaccgccc gccgcatggt ttaccacgcg ctgcaacatc cgctttatcg
     1741 cgatattgaa accattgtgc gtgtaaacgc gctggattcc gaatggggtg ttaacgacct
     1801 ggaagccgtc gttcgcggtg gtgcggacgt tgtgcgtctg ccgaaaaccg ataccgctca
     1861 ggatgttctg gatatcgaaa aagagatcct gcgtatcgaa aaagcctgtg gtcgtgaacc
     1921 cggcagcacc ggcctgctgg cggcgattga atctccgctg ggcattaccc gcgcggtaga
     1981 aatcgctcac gcttccgagc gtttgatcgg tatcgccctc ggtgcagaag actatgtgcg
     2041 caacctgcgt acagaacgct ccccggaagg aactgaactg ctgttcgctc gctgctccat
     2101 tttgcaggcc gcgcgctctg caggtattca ggcgttcgat accgtctatt ccgacgctaa
     2161 caacgaagcc ggattcctgc aagaagccgc ccacatcaaa cagctgggct ttgacggcaa
     2221 atcgctgatc aacccgcgtc agattgatct gctgcacaac ctctacgcac cgacccagaa
     2281 agaagtggat cacgcccgcc gcgtcgtaga agccgctgaa gccgccgctc gcgaaggcct
     2341 cggcgtggtt tccctgaacg gcaagatggt ggacggtccg gttatcgatc gcgcccgtct
     2401 ggtgctctcc cgtgcagaac tttccggcat ccgcgaagaa taaggcaatc aaaatgacgc
     2461 agaaaattga acaatctcaa cgacaagaac gggtagcggc ctggaatcgt cgcgctgaat
     2521 gcgatcttgc cgctttccag aactcgccaa agcaaaccta ccaggctgaa aaagcgcgcg
     2581 atcgcaaact gtgcgccaac ctggaagaag cgattcgtcg ctctggttta caggacggca
     2641 tgacggtttc cttccatcac gctttccgtg gcggtgacct gaccgtcaat atggtgatgg
     2701 acgtcatcgc gaagatgggc tttaaaaacc tgactctggc ctccagctcc ctgagtgatt
     2761 gccatgcgcc gctggtagaa cacattcgcc agggcgtggt tacccgcatt tatacctctg
     2821 gcctgcgcgg tccactggcg gaagaaatct cccgtgctct gctggcagaa ccggtgcaaa
     2881 tccactctca cggcggacgt gtgcatctgg tacagagcgg cgaactgaat atcgatgtgg
     2941 ctttcctcgg cgtcccgtcc tgtgatgaat tcggtaatgc caacggctac tccggtaaag
     3001 cctgctgcgg ctccctcggc tatgcaatgg ttgatgccga caacgcaaaa caggtcgtga
     3061 tgcttaccga agaactgctg ccttatccgc ataatccggc aagcattgag caagatcagg
     3121 ttgatctgat cgtcaaagtt gatcgcgttg gcgatgctgc aaaaatcggc gctggtgcta
     3181 cccgtatgac cactaacccg cgcgaactgc ttattgcccg tagcgctgcg gatgtgattg
     3241 ttaactctgg ctacttcaaa gaaggtttct ccatgcagac cggcaccggc ggcgcttcgc
     3301 tggcggtaac ccgtttcctg gaagacaaaa tgcgtagccg cgatattcgc gccgacttcg
     3361 ccctcggcgg tattaccgca acgatggttg atctgcacga aaaaggtttg atccgcaaac
     3421 tgctggatgt gcagagcttt gacagccatg ctgcgcaatc gctggcccgt aaccccaatc
     3481 acatcgaaat cagcgccaac cagtacgcta actgggggtc gaaaggcgca gcggttgatc
     3541 gtctcgacgt ggtggtactg agcgcgctgg aaattgacac ccagttcaac gttaacgtgc
     3601 tgaccggctc tgacggcgta ctgcgtggtg cttccggtgg tcactgcgat accgcgattg
     3661 cttctgcgct ttccatcatc gtcgcgccgc tggtacgcgg tcgtattccg acgctggtgg
     3721 ataacgtgct gacctgcatc accccgggct ccagtgtcga tattctggtc acagaccacg
     3781 gcatcgctgt taacccggca cgtccggagc tggcagaacg tctgcaggaa gcggggatca
     3841 aagtggtttc tattgagtgg ctgcgtgaac gtgcgcgtct gctgaccggt gaaccacagc
     3901 cgattgaatt caccgaccgc gtcgttgccg ttgtgcgtta ccgcgatggc tcggtgatcg
     3961 atgttgtgca tcaggtgaag gaataagcca tgcacctgct tcctgaactc gccagccacc
     4021 atgcggtgtc aattcccgag ctgctcgtca gccgggatga aaggcaagca cggcaacacg
     4081 cctggctcaa gcgccatcct gttccactgg tctcctttac cgtggttgcg cctgggccga
     4141 ttaaagacag cgaggtcaca cgccgaattt ttaatcatgg cgtgacagcc ttgcgtgcct
     4201 tagccgcaaa acagggctgg caaattcagg agcaggctgc actggtttcc gccagcgggc
     4261 cggagggcat gttgagcatt gccgccccgg ctcgcgacct caagctcgcc accattgagc
     4321 ttgaacatag tcatcctctc gggcggttat gggatatcga tgtcctgacg cccgaaggcg
     4381 acattctctc ccgccgcgat tattcactgc cgcctcgccg ctgcctgttg tgcgagcaaa
     4441 gcgcagctgt ttgcgcgcgt ggaaaaaccc atcaactgac cgatttactc aaccgcatgg
     4501 aggcgctgct aaacgatgtc gatgcctgca acgtcaacta aaaccacaaa gcttgcgacg
     4561 tcattaatcg atgagtacgc cctgctgggc tggcgcgcca tgctgactga agtcaacctg
     4621 tcaccgaaac caggcctcgt ggatcgcatt aactgcggtg cgcacaaaga tatggcgctg
     4681 gaagatttcc accgcagcgc gctggcgatt cagggctggt taccccgttt cattgaattt
     4741 ggtgcctgta gtgcagaaat ggcaccggaa gcggtactca acggattacg cccaatcggt
     4801 atggcttgcg aaggtgatat gttccgcgcc actgcgggcg taaacacgca taaaggaagc
     4861 attttttctt tagggctact gtgtgcggca attggccgtt tgcttcagct caaccagccg
     4921 gtaacgccaa caaccgtttg ttctacggcg gcaagtttct gccgtggcct gaccgatcgc
     4981 gaactgcgta ccaataattc acgactgacg gcaggtcaac ggttgtacca gcagcttggc
     5041 cttaccggcg cacgcggtga agccgaagcg ggttatccac tggtgatcaa ccacgccttg
     5101 ccgcattacc tcactctgct ggatcagggg ttagatcctg agctggcatt gctcgatacc
     5161 ttgctcctac tgatggctac caacggcgat accaacgttg catcgcgcgg tggcgagggg
     5221 ggcctgcgct ggctacagcg cgaggcacaa acattattgc aaaaaggggg cattcgaacc
     5281 cccgccgatc tcgattatct ccggcagttc gacagggagt gtatcgaacg aaatctcagt
     5341 ccaggcggca gtgccgacct gctgatcctt acctggtttt tagcacagat ttaattattt
     5401 aagcacttga taaatttgga aatattaatt ttcggagaac ccgtatgtct ttagcaaaag
     5461 ataatatatg gaaattattg gcgcccctgg tggtgatggg tgtcatgttt cttatccctg
     5521 tacctgacgg tatgccgccg caggcgtggc attactttgc agtgtttgtg gcaatgattg
     5581 tcggcatgat cctcgagcca attccggcaa cggcgatcag ttttattgcg gttactattt
     5641 gcgttattgg cagtaattac ctgctctttg atgccaaaga attagctgac ccagcgttta
     5701 atgcgcaaaa acaggcgctg aaatggggtc tggctggttt ttccagcacc acggtatggc
     5761 tggtatttgg cgcatttatt tttgcattag ggtatgaagt ttccgggtta ggccgtcgta
     5821 ttgccctttt cctggtgaaa ttcatgggca aacgcacgct gacgttgggt tatgcgattg
     5881 tcattatcga cattctgctg gcaccgttta caccgtccaa caccgcgcgt accgggggta
     5941 cggtttttcc ggtcattaaa aacctgccgc cgctgtttaa atcattcccg aacgatccgt
     6001 ccgcgcgtcg tattggcggc tatttgatgt ggatgatggt cattagtacc agtctgagtt
     6061 cgtccatgtt tgtcaccggt gcggcaccaa acgtgctggg tctggagttc gtcagcaaaa
     6121 ttgccggtat ccagattagc tggttgcagt ggttcctctg cttcctgccg gttggggtta
     6181 tcttgcttat cattgcgccg tggctttcct acgtgctgta caaaccggaa atcacacaca
     6241 gtgaagaagt ggcaacctgg gcgggtgatg aactaaaaac catgggtgcg ctgacgcgca
     6301 gagagtggac gctgattggc cttgtattgc tcagcttagg tttgtgggta tttggcagtg
     6361 aagtcattaa tgctactgcg gtaggtctgc tggcagtttc gctgatgctg gcgctgcacg
     6421 ttgtgccgtg gaaagacatt actcgctata acagcgcatg gaacacgctg gtcaacctgg
     6481 caacgctggt tgttatggcg aatggcctga cccgttctgg ctttatcgac tggttcgcca
     6541 acacgatgag tacgcacctg gaaggcttct caccaaacgc aacggtgatt gtactggttc
     6601 tggtgttcta ctttgcccac tacctgtttg ccagcctgtc tgcgcacacc gcaaccatgc
     6661 tgccggttat tctggccgtc ggtaaaggga ttccgggcgt accaatggaa caactgtgta
     6721 tcctgctggt gctgtctatc ggtatcatgg gctgtctgac gccttatgca accggtcctg
     6781 gggtgattat ttacggctgt ggctatgtga aatcaaaaga ttactggcgt cttggcgcaa
     6841 tcttcggggt gatttacatc tctatgctgc tgttggttgg ctggccgatt ctcgccatgt
     6901 ggaactaata cgttcgccaa acgctgaata aatcatcacg cccgccaggt aagcccactt
     6961 ggcgggcttt tttatgattt aatagataat attcagtgta tgtatgagtt ccacacccat
     7021 tatgaaagca ttctggcgta acgccgcgtt gctcgcggtt tctctgcttc ccttctcttc
     7081 tgccaacgcc gtggcgttgc aggcaaaaca gtatggcgat tttgatcgct atgtcctggc
     7141 cctctcctgg caaaccggat tttgccagag tcaacacgat cgaaatcgta acgaacgaga
     7201 tgaatgtcgc ctgcaaaccg aaacgaccaa caaagctgat tttttgaccg tacatggtct
     7261 gtggccagga ttgcctaaat cggttgctgc ccgtggtgtt gatgaacgcc gctggatgcg
     7321 cttcggttgc gctactcgcc caatcccgaa tctaccagaa gcgcgcgcca gccgaatgtg
     7381 ttcatcgccg gaaaccgggt tatcactgga aacggccgct aaactaagtg aagtcatgcc
     7441 aggagctggc ggacgttcct gcctggaacg ctacgaatat gccaaacacg gtgcctgctt
     7501 tggttttgat ccagacgcat acttcggcac gatggtacgc ctgaatcaag aaattaaaga
     7561 gagcgaagcc ggaaaattcc ttgcggataa ctacggtaga acagtgagcc gccgtgactt
     7621 tgatgccgcc tttgccaaaa gctggggaaa agagaacgtg aaagcagtta agctaacgtg
     7681 ccagagtaac cctgcgtatt taactgaaat tcagatctcg atcaaagctg acgccatcaa
     7741 cgctccgctt tctgccaact cattcttgcc acaaccacac ccaggcaact gtggcaaaac
     7801 ctttgtgatt gataaagcag gttattaatc ctctgattta caaaacacgc taattcgtct
     7861 tccataaacc atgacgatta agatttatac ataatgcgct acattgagga attccctgaa
     7921 tgtgacgcaa atcacttcaa agagtgagca acagccagta tcatcatgtg agttaaaccc
     7981 tcgccgcctg acggtgaggg ttttcttttg ggtatgttta tctgcgacac tcgcagtacc
     8041 gacgacatgg agtaaaaatg tccagaccaa ctatcatcat taacgacctg gatgccgaac
     8101 gcatcgatat tctgctggag caacccgcct atgctggttt gccaatcgcc gacgcgttaa
     8161 acgcagagtt ggatcgcgcc caaatgtgtt cgccagaaga gatgccacac gacgtggtga
     8221 caatgaacag ccgggttaaa ttccgcaatc ttagcgatgg cgaagtgcgt gtgcgcacgc
     8281 tggtgtatcc ggcaaaaatg accgacagca atactcagct ttccgttatg gctccggtag
     8341 gtgccgcact gctggggctg cgcgttggcg attccattca ctgggaactt ccgggcggcg
     8401 ttgcaaccca ccttgaagtg ctggaactcg aataccagcc agaagctgct ggcgactacc
     8461 tgctttaatc ccatctgcac aagcgataac tcgctttgcc cagaggatgc actcgcatcc
     8521 tctttccgct caaatatcaa ccagatttat cattttgctc ttaagcgcag cattctaatc
     8581 tgaactggtt attcatctgc ggaacctcat atgagcgaag ctctcggtat cactgcattt
     8641 tatatattca tagccgcaat tattgttgct gcagtgctat accttgaaca acgctggtag
     8701 cgcctggttt tatttatttt ccagctcaat agcaaattca gatcaaatca gcctcgcaaa
     8761 ttatagattc ataaccaata atgtgctgtt atgtatacat gcaaaaacaa ggagagaatg
     8821 cttatgtaca aaacaatcat catgccagtg gatgttttcg aaatggaatt gagcgacaaa
     8881 gcgattcgcc acgctgaatt cctcgcccag gatgacggag ttattcatct acttcacgta
     8941 ctaccggggt cagccagcct gagcctgcac cgttttgccg ctgatgtacg tcgttttgaa
     9001 gagcatctgc aacatgaagc agaagaacgt ttgcaaacga tggtcagcca cttcaccatc
     9061 gatccttccc gcattaagca acatgtccgt tttggtagtg tgcgggatga agttaacgag
     9121 ttagcgaaag aacttgatgc agatgtagta gtgattggtt cgcgtaaccc atcaatttca
     9181 actcatctgt taggttctaa cgcctcgagc gtaatccgcc acgccaattt gccggtgctg
     9241 gttgtgcgtt aattgccaga ataagtctcc cgccctgccc ggagcatccc tgcagggctt
     9301 tttaatgcaa taaaaaagcc gccaggtttg accccggcgg ctaagcaatt acaggtgaat
     9361 cttacttctt cttatgcagt tttggtgcga atcaggtagt caaaagcact cagagaggct
     9421 ttggcacctt cgccagtggc gatgatgatc tgcttgtacg gaaccgtcgt acagtcaccc
     9481 gctgcgaaca cgcctttgac gttggtttcg catttcgcat caatgataat ctcgcccatg
     9541 cgattacgtt cgactgcgcc ttccagccag ttggtgttcg gcagcagacc aatctggacg
     9601 aaaataccgg ccagttcgat gttgtgaata tcgccgctga cacgatcgcg atattccaga
     9661 cctacgactt tgctaccgtc gcctttcact tccgtggttt gcgcattcag aataatgtcg
     9721 acgtttttca ggctgcgtag tttgtcctgc agaacctggt cggctttcat ttctggcgca
     9781 aattccagca gcgttacgtg ctcaacgata cccgccaggt caattgccgc ttccacgccg
     9841 gagttaccgc cgccgataac cgctacgcgt ttgcctttaa acagcgggcc gtcgcagtgc
     9901 gggcagtagg tcacgccttt ggtgcgatac tgatcttcgc caggaacgtt catgttgcgc
     9961 cattttgcac cagtcgccac gataatgctg cgtgctttca gtaccgcgcc agaagctgtt
    10021 tcaatctgat gcaggccgcc ttcaaccgct gccgggatca gtttgctcgc gctctggctg
    10081 tcgatcacat caacgtcgta ttcatcaaca tgaactttca gcgcacctgc cagtttctgg
    10141 ccttcggtct tcggtacaga aatgtagttt tcgatatcaa cggtatcgag gatctgacca
    10201 ccaaaacgtt cgccaatcag accggtacgg atgcctttac gtgcggagta aattgctgcc
    10261 gctgcacccg ccgggccgga accaacgatt aatacgtcat aagcatcacg cttgttcagc
    10321 tcttctgccg cacgtttttc cgcaccagta tcaattttgg caacgatttc agtcaacgtc
    10381 atgcggccct gaccaaactc tttcccgttt acgaacactg ccggaacgcc catcacgttg
    10441 cgatcggtga tttcgttctg gaaggtgccg ccgtcaattg cagtgtgctt gatgcgcggg
    10501 ttcagtacgc tcatcaggtt cagcgcctgc accacgtccg ggcagttgtg gcaagagagc
    10561 gagtaatagg tttcgaattc aaaatcaccg tcaatatggc gaatctgctc cagcagagac
    10621 tgcgcttctt tcgacggatg accgccagtc cacaacaacg ccagtaccag cgaggtgaac
    10681 tcgtggccca gcggggagcc tgcaaaacgt ggtccctggt tggaacctgg gttggtgatc
    10741 aggaaagacg gcttacgcac tggcaagctg ttatcttctt taaaggtgac tttgtctgac
    10801 agttctgcga tttcagccaa cagttccttg atttctgccg atttagcgct gtcatccagc
    10861 gtggcaatta actcaacagg cttggtcaat ttctcaaggt aagccttgag ttgagttttc
    10921 atatttgtgt cgagcatgtt tatctcctgg gctttaaaca tcatcatgca agcggcctct
    10981 gcggctagct ggatgcagct tgcatcatgg tgcggggaag aaaatcgggt gccgcagcac
    11041 ccgaagaatt aggtgaattt ccgaagtatc tcgcgccgga gttagtagcg cgaaagacaa
    11101 aggaaattta gattttacca accaggtcca gagacggagc cagagttgct tcaccttctt
    11161 tccatttagc cgggcaaact tcacctgggt gagaagctac gtactgtgct gctttgattt
    11221 tacgcagcag gtcagacgcg tcacggccaa tgccttcagc ggtaacttcg attgcctgga
    11281 tgataccctg cgggtcaaca acgaaggtcg cacggtcagc cagaccttca tcttcacgca
    11341 tgttgtcgaa gttacgggtc agggcgccag tcgggtcgcc gatcatcgca tatttgattt
    11401 tagcgatggt ttcagagctg ctgtgccatg ctttgtgggt gaagtgagta tcggtagata
    11461 ctgcgtatac gtctacgccc agtttctgca attcttcgta atggtcagca acgtcaccca
    11521 gttcggtcgg gcatacgaaa gtaaagtcag ccgggtaaaa gaagaagacg ctccagcggc
    11581 cttcggtatc tttttcggtg atttcgatga attcgccgtt tttgaatgcc tggtttttaa
    11641 aaggtttaat tttggtgtta atcaaggaca tctatacttc ctccgtgttt tcgatgagat
    11701 gtaaggtaac caatttttgc cgattcggct aatgcgtttc cattatcaaa tcgataagtt
    11761 ttaccttaca accttcgtaa gacaactttg tgaactttgc accgccagcg gcgataaaaa
    11821 gtaaaatggc cccctttcca ggtgaccgtg taatctgaac taccctttgg tatcttcagc
    11881 gcctgcctgc aggctgaggt gttgccctta caaatgcaac aacgacatgg attacaacac
    11941 cctcataaat aaagggcaat cacctgatct aagctcttac ctatgacagt gataggttat
    12001 gccttttact cgacttttgc actgactgaa aaggacaaat taatgttaaa aaagatactt
    12061 ttactggccc tgcttcctgc aatcgccttc gcagaggaac ttcctgcccc agtaaaagcg
    12121 attgaaaaac agggtattac aatcatcaaa acgttcgatg cccctggagg aatgaaaggt
    12181 tatctcggta agtatcagga tatgggcgtc accatctacc tgactccaga tggtaagcac
    12241 gctatctctg gttacatgta caacgaaaaa ggtgaaaacc tgagtaacac gcttatcgaa
    12301 aaagaaattt acgcacccgc cggacgtgaa atgtggcaac ggatggaaca atcccactgg
    12361 ctcctcgacg gtaaaaaaga cgcgccggtc attgtctacg tcttcgccga cccgttctgc
    12421 ccatattgta aacagttctg gcagcaggct cgtccgtggg tagattctgg caaagtgcaa
    12481 ttgagaacat tgttggttgg ggttatcaag ccagaaagcc cggcgacagc agcggcaatt
    12541 cttgcctcca aagatcccgc aaaaacctgg caacaatatg aagcctctgg tggcaagctt
    12601 aagctaagcg tggctgcaaa cgtaagtaca gagcaaatga aagtgttaag cgacaacgag
    12661 aaactcatgg acgatctggg ggcaaatgtc acaccagcta tctattacat gagtaaggaa
    12721 aataccctgc aacaagccgt cggattaccc gatcagaa
//
LOCUS       DAZFJL010000130        12503 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFJL010000130 DAZFJL010000000
VERSION     DAZFJL010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.132"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            1..381
                     /locus_tag="ACHJ8C_003743"
                     /pseudo
     CDS             1..381
                     /locus_tag="ACHJ8C_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(650..1384)
                     /gene="cysH"
                     /locus_tag="ACHJ8C_003744"
     CDS             complement(650..1384)
                     /gene="cysH"
                     /locus_tag="ACHJ8C_003744"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO5553529.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRLAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1458..3170)
                     /gene="cysI"
                     /locus_tag="ACHJ8C_003745"
     CDS             complement(1458..3170)
                     /gene="cysI"
                     /locus_tag="ACHJ8C_003745"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO5553530.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3170..4969)
                     /gene="cysJ"
                     /locus_tag="ACHJ8C_003746"
     CDS             complement(3170..4969)
                     /gene="cysJ"
                     /locus_tag="ACHJ8C_003746"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO5553531.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     VTGTVNEIHTSPYSKDAPLAASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPTN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5288..5650
                     /gene="queD"
                     /locus_tag="ACHJ8C_003747"
     CDS             5288..5650
                     /gene="queD"
                     /locus_tag="ACHJ8C_003747"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO5553532.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPQGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5728..6999
                     /gene="ygcN"
                     /locus_tag="ACHJ8C_003748"
     CDS             5728..6999
                     /gene="ygcN"
                     /locus_tag="ACHJ8C_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5553533.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILHARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLTLEPSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLRSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRVWSGLMQDISRDLWDQGNTP
                     VPPLRQLLWRHLRRHGLWHLTGDVIRSLRCL"
     gene            6990..7250
                     /gene="ygcO"
                     /locus_tag="ACHJ8C_003749"
     CDS             6990..7250
                     /gene="ygcO"
                     /locus_tag="ACHJ8C_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO5553534.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQTAQRLINACPAGLFSLTPE
                     GGLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7267..7842
                     /gene="ygcP"
                     /locus_tag="ACHJ8C_003750"
     CDS             7267..7842
                     /gene="ygcP"
                     /locus_tag="ACHJ8C_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO5553535.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7990..8850)
                     /gene="ygcQ"
                     /locus_tag="ACHJ8C_003751"
     CDS             complement(7990..8850)
                     /gene="ygcQ"
                     /locus_tag="ACHJ8C_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO5553536.1"
                     /translation="MNIVLVTINQDHAAIASWLAAQDFSGCTLAHWQIEPQPMIAEQV
                     LDALVEQWQRTPAEVVLFPPGTFGDELATRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGADKNATLPSGMQQLNIVPGALPDWLISVENLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKMGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8847..9626)
                     /gene="ygcR"
                     /locus_tag="ACHJ8C_003752"
     CDS             complement(8847..9626)
                     /gene="ygcR"
                     /locus_tag="ACHJ8C_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO5553537.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEDAVLLETAADLRFAPE
                     FVARHIAEWQYQNPLDLIITGCQSSEGQNGQTPFLLAEMLVWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEITRETVAAEAPA
                     IQCLQLARPEQRRGATLIDGQTVAEKAQKLWQYYLCQRMQP"
     gene            complement(9604..11013)
                     /gene="ygcS"
                     /locus_tag="ACHJ8C_003753"
     CDS             complement(9604..11013)
                     /gene="ygcS"
                     /locus_tag="ACHJ8C_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5553538.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFMLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVDHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISESPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     RFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQATLLLLAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(11035..12489)
                     /gene="ygcU"
                     /locus_tag="ACHJ8C_003754"
     CDS             complement(11035..12489)
                     /gene="ygcU"
                     /locus_tag="ACHJ8C_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO5553539.1"
                     /translation="MSLSRVAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYVLEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAQATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPYADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
BASE COUNT         2867 a         3443 c         3206 g         2987 t
ORIGIN      
        1 agtgatatcg tcaatcactt cttcagccgc gcagcccggt tgttcccggt cggtatcagc
       61 cagataatca cagataacgg aaaagagttc ctgggaaact tcgacaaaac gctgcaggaa
      121 gccgctatca aacacctctg gacctatccc tacacgccaa aaatgaacgc tatctgtgaa
      181 cgttttaacc ggacgttaag agagcagttt attgaattta atgagatttt actctttgaa
      241 gatctggcgc tatttaacca gaagctggcg gaatatctgg tgctgtataa cagcaaaaga
      301 ccccacaagg cgctcgcact aatgacgccc gtggaatata ttttaaaaga gaataaaaat
      361 tgcaatatgt ggtggaccca tacaatgtct tgcctccctg caaagaagag actaacatcc
      421 gaattgctac gttcgaacgc gagcctcagg ttgatagaaa tatcgcctgg ggcttttgtc
      481 catctggaac ctcgcgaatg cttaacgcca gacagcctca agcacccaat gccattctat
      541 acctgttaat tctccctgcg acgttctcgt ttaaatgccg gaatgctgct cgcaaaataa
      601 aaacgccccc atcatttctg acagaggcgt ttaatttatc cggcaatatt tacccttcgt
      661 gtaatccaca ttcccttttt aagccaaaga aacgcgtttc ttcttccgcc atgccgggtt
      721 cccatttacg ggttgtatgg gtatcgccca ccgataaata tccttcatcc cataatgggt
      781 gatatttcag gccatgtttt tgcaggtact gataaatagt tcggttatcc cagtcgataa
      841 tcggcagcac tttaaatacg ccacgctgaa ttgccagcac cggtaaattg gcgcggctgc
      901 cggattgttc acggcgcagg ccagcaaacc aggtttgcgc attcagttct ttcagcgccc
      961 ggttcatcgg ttcgactttg ttgatgtcat tgtacttttc aatgccttca acgccctgct
     1021 cccacagttt gccgtagcgt gcttcctgcc aggcggcgct ttcggtagca cggtacactt
     1081 tcaggttgag cttgagtttg tccgttaact cgtcaataaa gcggtaggtt tccgggaaca
     1141 gatagccggt atcggtgagg atcactggaa tatccgggcg aatctgattc accaaatgca
     1201 gactcaccgc cgcctgaatg ccaaagctgg aagaaagtac atattcaccg ggcagattat
     1261 ccagcgccca ggctaggcgg ccttcagcgt ccagtttttc cagttgggcg ttagtttccg
     1321 ccagcgccag aatccggtct acctttggca gttcgttcag ggcgtttaga tcgagtttgg
     1381 acataagttc ctcactgttt gccttgcctg atgcaacgct tacacatctt atcaggccta
     1441 caagaccggg ctgatggtta atcccacaaa tcgcgcgccg gatcgagcac cgggcgaatg
     1501 atgcccgcac gcaccgtaaa gtcgccgaag ccttcacccg cttcgcgctc tttcgcccag
     1561 cgccctatca gttcatcaag cgacgccagg atttccggct cggtgatgtt ttctttatac
     1621 atccgtggga tacgtgtccc aatgcggttg ccgccaagat gcaggttgta gcgacccggc
     1681 gctttcccca ccaggcccac ttccgccagc atcgcgcgac cacaaccgtt cgggcagcct
     1741 gttacacgca tcacgatatg ctcatcgctg acaccatgtt tcgccattaa attatcgatg
     1801 ttgtcgataa aagacggcag gaaacgctcc gcttccgcca tcgccagcgg gcaagtcggg
     1861 aatgacacgc aggccattga gttttcacgc tgcggcgtga cggcattcat taacccgctt
     1921 tctttggcga tcttctcgat cttcgctttc tcgctttccg gcacgccggc gatgatcaga
     1981 ttctggttcg ccgtaatgcg gaaatcacct ttgtggatct ttgcgatctc cagcaagccg
     2041 gttttcagcg gccgccccgg ataatcaaga attcgaccat tttcgataaa cagcgtcagg
     2101 tgccagttat catcaatgcc cttaacccag ccaatacgat ctccgcgtcc ggtaaactca
     2161 tacgggcgaa tcggctcgaa tttgatcccc gcgcgacgtt caacttccgc tttaaacgtc
     2221 tcaaccccca cgcgctccag cgtgtatttg gttttggcat ttttacgatc ggttcggtta
     2281 ccccagtcac gctgagttgt cacgacggct tccgccaccg ccagcgtatg ctccagcggc
     2341 agatagccaa actcactcgc cgtgcgggcg taggttttct tgttgccgtg ttcgatggaa
     2401 agcccaccgc ccaccaacag gttaaagccc accagcttgc cgttttcggc gatcgccacg
     2461 aagttcatgt cgttggcgtg cagatcgata tcgttctgcg gcgggatcac taccgtggtt
     2521 ttgaacttac gcggcaggta ggtctgacca aggatcggct cttcatcagt agtggcgact
     2581 ttttcctgat cgagccagat ctccgcatac gcgcgggtac gaggcagcag atgttcagaa
     2641 attttcttcg cccattcata tgcttccgca tgcagctgcg actcgtaagg gttcgaggtg
     2701 cagagtacgt tacggttcat gtcgttggcg gtcgccagcg catcaagacc gaccgagtgc
     2761 agcatctggt gcaccggttt gacgttcttt ttcagaatgc cgtggaactg gaatgtctgg
     2821 cggttggtca ggcgaatgct gccatagatg gtgttttcac cggcaaattt gtcgatcgcc
     2881 tgccactgtt tagtggtaat caccccaccc ggcagacgac agcgaagcag catcgcgtgg
     2941 cgcggctcca gcttctgttc agcacgttcg gcgcgaatgt cgcggtcatc ctgctgatac
     3001 atgccgtgga agcggatcag caggaagtta tcgcccttaa agccgccggt cagaccgtcg
     3061 tttaaatctt ccgcaatggt gccgcgcagg tagttgcttt caagcttcat gcgctcggcg
     3121 tctgtcagtt ttccttcgac cactaaaggc cctggatgtt tttcgctcat tagtagacat
     3181 ctcgctgata acggcgctct acgcgcagct cacttaaaaa ttcatccgcc gcttcggtgt
     3241 ccatgccacc aaattcggca atcacttcca gaagtgcctg ctcaacgtct ttcgccatgc
     3301 gattagcgtc gccgcagaca taaatgtggg caccgtcatt gatccagcgc cacagctctg
     3361 cgccctgttc gcgcagtttg tcttgtacgt aaattttttc tttttgatcg cgcgaccagg
     3421 cgagatcgat gcgcgtcagc acgccttctt tgacgtaacg ctgccattcc acctggtaga
     3481 ggaaatcttc cgtaaagtgt gggttgccaa agaatagcca gtttttaccc ggcgcttcgt
     3541 cggcagcacg ctgctgcata aaggcacgga acggcgcgat gccggtgcca gggccaatca
     3601 tgatcaccgg ggtttccgga ttggttggca ggcggaagtt gtcgttatgt tcgataaaga
     3661 cgcggacttc accctcttct tccacacgat ccgcgaggaa gctggaggca ccaccggcac
     3721 gggcgcggcc ttccacgtcg taacgcacca caccaacggt aacgtgcact tcgttctcga
     3781 cttccgcctg cgaggaggcg atggaataca ggcgcggggt cagcgggcgc agcagattaa
     3841 tcagcgcttc ggcatccagt tgcgccggag agaaacgcac catgtcgaca atcggcgtcg
     3901 tcgcggcgta atgctgtaac ttcgctttat cgcccaccag cggcagcagc gtttcgctgc
     3961 gggtaagcgt ggcatagttc tcaacaatgt tggcggtgtt gacggtcagt tcgaagtgcc
     4021 actgtagcgc ttcgttcaga ggcaacgttt tgccctcgac ggtgacaggt tcatcgcctt
     4081 tcagccacag cagttcgaca agttctttca ccagtgccgg atcgttctga taccagacac
     4141 ccagcgcgtc acccggctgg taacgcaggc ccgagtcacc taagtcaatt tcgatatggc
     4201 gaacgtcttt ttcagagtta cgcccggtaa ttttctggtt aaccgaaagg ctggccgcca
     4261 gcggcgcgtc tttgctgtac gggctggtgt ggatttcatt taccgtgcca gtaacgacgg
     4321 attgcgaagg tgccgcgaca ggcgcacgcg atttaagcgc atcaaccacg cgggcgcgcc
     4381 actcgctggc agcagcctgg tattcaacgt cggcatcgac acggtcgagc aggcgttcac
     4441 cgcccagttc cgccagctta ctgtcaaaat ctttcccgga ctggcagaaa aattcataag
     4501 agctatcgcc gaggctaaac acggcaaacg cggtgttttc cagctttggc gcttttttgg
     4561 agaacaggaa cttatgcagc gcgacggctt cttccggcgg ttccccttcc ccttgcgttg
     4621 acgtcaccac gataagcagt ttttcgctgg cgatttgttt gaatttatag tcgcccgcgt
     4681 tcaccagctt aacgttcagt tttgccgcta ataaatcatc gcgtaatgct tcagcaaccc
     4741 gacgcgcatt gccagtttgc gaggcggaga taatagttat acccggcatt tctgcggcgg
     4801 gcgctggcgt cgctgcaagc gcagcaggct gctggttgag cacgccccag aaatagccag
     4861 aaacccaggc aagctgggta ggagttaaat cggtcgtggc cgcctgaagg cgtgccagtt
     4921 gctccgggtt caacggaagc aacgcggaag gtgggacctg tgtcgtcatg cgtcgttatg
     4981 ttccagtaag caaagctgtt tctgcgccct gtcagcgccc ataaaacaga agagaaggta
     5041 aggttaacgg ggtgaacggt gtggattaaa gacgggatag cgataactaa taaccaaatc
     5101 gactaacctg ttttagcaat agtctttaac aacaaaatag attaaccaac ccaatgaaaa
     5161 acaaatgaat attgcccatc aataagataa atcagcgatt ttgcgcaaca aagtcgtttt
     5221 agataatgcg aaaaaacagc ctttccggta ctctacggcg gttttatcgt catgtagaga
     5281 aattatgatg tccaccacgt tatttaaaga tttcaccttc gaagccgctc accgcttgcc
     5341 gcacgtccca caagggcata aatgtggtcg cctgcacggg cattccttta tggtgcgact
     5401 ggaaattacc ggggaagtcg atccgcatac gggctggatt atcgatttcg ctgaactgaa
     5461 agcggcgttt aaaccaactt atgagcgcct cgatcaccat tatctcaatg atattccagg
     5521 tctggaaaac ccaaccagcg aagttttagc aaaatggatt tgggatcagg ttaaacccgt
     5581 tgtgccgctg ttaagtgcgg tgatggtaaa agaaacctgc accgcaggtt gtatctatcg
     5641 cggcgaatga taagagtgtg tcggcggtca atttccctta agtaacgcta tgttagggtg
     5701 ttgtgttctg gatatctggg gcatgacatg gaagacgact gcgacattat tattatcggt
     5761 gccggaattg ctggcaccgc ttgcgcgtta cgctgcgcgc gagcgggttt atccgttttg
     5821 ttactggaac gagcggagat tcccggcagc aaaaatcttt ccggcgggcg tttatatacc
     5881 catgcgctcg cggaactcct cccgcaattt cacctgaccg cgccccttga acgacgcatc
     5941 actcacgaaa gtctttctct gttaacgcca gatggcgcaa cgacgttttc cagcttacag
     6001 cccggcggtg aatcctggag tgtattacgt gcacgattcg atccgtggct ggttgccgaa
     6061 gccgaaaaag aaggtgtcga atgcatcccc ggtgcgacgg tagatgcact gtatgaagaa
     6121 aacggcaggg tgtgtggtgt catttgtggt gacgatattc ttcacgcccg ttatgtggtg
     6181 ctggcagaag gtgccaacag cgtcctggcg gaacgccacg ggttagtgac tcgtcctgct
     6241 ggcgaagcga tggcattggg aatcaaagaa gtgctgacgc tggaaccatc cgctattgaa
     6301 gaacgttttc atctggagaa taacgaaggc gcagcgttgt tgttcagcgg cgggatctgt
     6361 gatgacttac ccggcggcgc atttctttat actaatcaac aaacgctctc gttagggatt
     6421 gtttgcccgc tctcctccct tacgcaaagt cgtgttccgg caagcgagct gctgactcgc
     6481 tttaaaacgc atccggcagt gcgcccgctt atcaaaaaca cggaatcact ggagtatggt
     6541 gcgcatctgg tgccagaagg tggcctgcgc agtatgccgg tgcaatacgc cggtaacggc
     6601 tggctgctgg tgggcgatgc gttgcgcagt tgcgtcaata ccggaatttc cgtgcgcggt
     6661 atggatatgg cgctaactgg cgcgcaggcg gcggcacaaa cgctgataag cgcctgccag
     6721 caccgcgagc cgcaaaatct gtttccgctt tatcatcaca acgtagagcg cagcctgctg
     6781 tgggatgttc tacagcgtta tcagcatgtt ccggcgcttt tgcaacgccc tggctggtat
     6841 cgggtgtggt ctgggttaat gcaggatatt tcccgtgatt tatgggatca ggggaataca
     6901 cctgttccac cgctgcgcca gttgctctgg cgtcatttac gtcgtcacgg cttgtggcat
     6961 ctgacgggcg atgttatcag gagtctgcga tgtctgtagc ccgtaatctc tggcgcgctg
     7021 ccgatgcgcc gcacattgtt ccggctgact ccgttgagcg ccagacggca caacggttga
     7081 ttaacgcctg tccagcaggt cttttttcgc tcacacctga aggtggctta cgtgttgatt
     7141 atcacggctg cctggagtgt ggcacctgcc gtttgctgtg cgacgaatca acactacaac
     7201 agtggcgcta tccgccttcc ggattcggca tcacctaccg ctttggataa ataataagga
     7261 taatttatgc ccctcttaca cctgctccgc cagaatccgg tgattgctgc cgttaaagat
     7321 aatgccagcc tgcaactggc aatcgactct gaatgccagt ttatttccgt gttgtacggc
     7381 aatatctgca ccattagtaa tatcgttaaa aagattaaga acgccgggaa atatgctttt
     7441 attcatgttg atttgctgga aggcgcgtca aataaagaag tggtgattca gtttttgaaa
     7501 ctggtaaccg aggcggacgg aattatcagt accaaagcct caatgctgaa agcagcaaga
     7561 gcggaaggtt ttttctgtat tcatcgactg tttattgttg attcgatttc gtttcacaac
     7621 attgataagc aagttgcgca atcgaatccg gattgtattg agatcctgcc aggctgcatg
     7681 cctaaagtgc tgggctgggt gacggagaaa atccgccaac cgctgattgc tggcgggctg
     7741 gtgtgcgatg aagaagatgc gcgtaatgcg attaacgcgg gtgttgtggc gctttccacc
     7801 acgaatactg gggtctggac gttagcgaaa aaattgcttt gacgggataa tcgtaacaag
     7861 ttgaatttgg tgtgatttgt aggctgcacg ccacatccga cattcagcgc ctgatgcgac
     7921 gcttgacgcg tcttatcagg cctacaagtc cccagcccag aaccatcggc aggatcatcc
     7981 ggcagcacgt tattggcaat ctgcgtgaat gtttgtcacc agtgcctcca gcaccacttt
     8041 ccagtcatca accacgccaa catctgcctg tgaaaatacc gctgcactgg cgtcatgatt
     8101 aatcgccacc acaaacttgc tattgcgtac gcccgccatt aacgctgctg cgccggaagc
     8161 gccaacaaca atacacacct caggtgccag cagatgtccg gaaataccaa tcactttttc
     8221 ggcatcaacg ccaccgttca tcactcgtgc ccgactgtag cccacttcgg ctcccatctt
     8281 ttctgccagc atagcgattt cctggttatc tgcctctccg ccctgcccca ccaccagcac
     8341 ccgtctggcc tcagctaatg ggtcacgggt aacatttttc aggttttcga cgctgataag
     8401 ccagtctgga agcgcgccgg ggacaatgtt cagttgctgc atcccagagg gcaacgtggc
     8461 gtttttatct gctcccgcct gacgtgccag cgaaaggcat agcgggcgtt tttctgtttg
     8521 cagcgtcgcc gtcaacgcat ttccccagtg ggatttgcgc acgcttacgg ttggaatatc
     8581 taacgaagtc acctggcaga tgcttgcacc atgtaaacgc caggccagtc gggtagccag
     8641 ttcatcgcca aacgttcccg gcggaaagag gacaacctcc gcaggcgttc gttgccactg
     8701 ttccaccagc gcatccagca cctgttccgc aatcatcggc tgtggttcta tttgccagtg
     8761 tgccagggta caaccgctga aatcctgcgc cgccagccag ctcgctattg cggcatggtc
     8821 ctgatttatt gtcacaagta cgatattcat ggctgcatcc tttggcataa atagtactgc
     8881 catagctttt gcgctttttc tgctaccgtt tgcccatcaa tcagcgtcgc tccgcgtcgt
     8941 tgctctggcc ttgccagttg caggcactgt atcgctggcg cttccgcggc aacagtttcg
     9001 cgggtaatct ccgctttccc cgccgccatt cgctggcgca ttcccggtac tggcaaggcc
     9061 acttcaccac actgacgcac ggcaatcacc gcaggcaagc gaacccggca acagcgcagt
     9121 ccatgttcag tacgctgttc gagggtgata aacggcgcgt cgagagtaaa gcgctccacc
     9181 tgggtgaagc agggccagac cagcatttcc gccagcaaaa atggcgtttg cccattctgc
     9241 ccttcgctgc tttggcagcc agtaattatc agatccagcg gattctggta ctgccattcg
     9301 gcaatatggc gagcgacaaa ttccggcgca aagcgcagat ctgccgccgt ctccagcaat
     9361 acagcgtcct caaacccaag tgccatgaga tagcgcagcc agtgcaacgc ccgttcatcc
     9421 cccatgctca acgcggttaa agacatcggc gtaccgtttt ttctctgcgc caacagcagc
     9481 gcggcggcag cctgttcatc agcaccgagt aaacttcgca gtagcgaaac atccggtccg
     9541 ctattaccct gagccgccgc ctgccactct ttttccgcca gcattccggc atccggttcg
     9601 gctttaaacg ctaacagaat gttcattcgc acctcctaca tttcccgccg ccaccagcgg
     9661 gagtgctttg gtttctggtg cccatagcca ggtcacaaca aaaccaacca acaacactgc
     9721 cgccaggagc aataaggtgg cttgcattcc ccactgcgcc agcacccacg gcagcaggcc
     9781 agtacttacc gccgccccca accgactcat ggcggtggca aaaccgacgc ccagcgagcg
     9841 aatgtcagta ggaaaacttt ccgcaggcaa aatgcccacc agattactga ctgccgaaat
     9901 ggtggtgctg aagagaacaa aaagcagcag cgttaatgaa ctgccggaag gcaaacaggc
     9961 catcacgacc agcgttgccg ccagcagcaa aaaacttccc agtaaaaatc tgcgatgcgc
    10021 taacaagtgg gtcaacacta accctaataa cgcgccaaca attaacaacg cattaagcat
    10081 caggctggca gtcagcgcat cttccagacc aatagtttgg gcgatggtcg gcagccaggt
    10141 ataaatcaca aaccatggga ttacgaggca gacaaagaag acgctgttaa acgccgtgcg
    10201 ccgccagtag cgcgaagaga acaaggtttt gatgtgttta tgggtcgccg ttgccacttc
    10261 atcgcccagt aaaacatgcg ggccaaaata gcgatgaacg atagcgtgag cttctgcaaa
    10321 acgcccctgg cgcagtagcc agcgtggcga ttccggcgtg ccccaacgta gcagcgtaat
    10381 cagcaaggct ggtaacgctg ctgatgccag cagccagcgc caggcctccg ggctttcgga
    10441 aataaagtga tgtccggcaa tacttgccag cacatagcct acggtccaca ccacactgaa
    10501 tgcgcccagc aaaataccgc gatggcggcg cggggaaaat tcagccagca aggtgtgatc
    10561 gactgagtaa tcgcctccca gcccaatgcc gatcaaaatg cgcaatccaa taagatgctc
    10621 tggcgtggtg gcaaaaaact gtaaaaacga agcaagcgta atcagcataa aactgaaggt
    10681 gaagattttt tgccgcccga tatggtcgga gatccatcca agaaccaggc tgccaagaaa
    10741 caaaccaagg agtgctgagc cgccaatcat tcccgccata aacggcgtca gttgcatggc
    10801 gggcgtaagc tgaataatgg cgtaaccaat gacgccgagg acataaccgt cggtcaggtg
    10861 cgcaccgaaa gtgagcgcag caatgcggca gtgaaaacgg ttaagtggta aatcatccat
    10921 tcgcaccggt gaagtgttca tctttacccc ttcattgtcc ggtaataaga acggcttctc
    10981 gcctgagaag ccgaaaaggc aacgccccgt catcgaggaa cgctgcctga cacattattt
    11041 ttcaatcgga tagatagtac ccgtattcat aatgccgtta ggatcgaact gctttttcag
    11101 cccttccagc aacgcccacg cgctgccgtg ttccagtttg ctccagtgaa cgcgatgttt
    11161 accgataccg tggtggtgca ccatcgaacc gccgagacga atggtttctt cgcagatgat
    11221 tttgttgagc ggattgtggt acttgtcgat ttcctcttcc ggcttacagt caacgacgtt
    11281 gtagtcgtag acgaagtaca tgttggtgcc gttctgatag ctatgagagg agtgaccgcc
    11341 cagcatggtg atgtcatcgg cgtacgggaa ctcggtacga atacggttaa taacgctttc
    11401 gtagatttcg tgaatgcagc tccagcagcc ggacacttca gtggtaaagc ccatgttgcc
    11461 ggttttgagg atctgcacac gttcggcagc cactttatcc ggtccccagt tcaggttgtt
    11521 aaaccaggtt tcgatcagct tgctgtccac gcgctggcat tgcgggtagc gggcaacgat
    11581 ttccgcaatc ccttcgcccg tagcctgtgc gatgcgcgga ttgccttcag ccataaagat
    11641 cagcacacat tttccgtcgg caaaatgggt gaagtgctgc gtaccatctt cagcgtcata
    11701 caggcgagcg atcgacggac gatacccttc caccatgatt tcacgcagga tgttgaagcc
    11761 ggttttcatg tcttccagca catagccata gaagagattg ttttccggcg taaatttgaa
    11821 gattttcact gtgacttcag tgatatagca tagtgcacct tcgttgccga tgatgatatg
    11881 acgaatgtcc gggccagccg cgcgacgtgg cacgttttta atgcgtgtga cggtgccatc
    11941 tgccagcacc gcttccagac caacgaccat atcctcgatt gcgccgtaga gtgtggaaaa
    12001 ctgcccgata ctgcgggtcg ccaccaggcc gcccatctgc gccagcggct ttgactgcgg
    12061 agaatgcccc gtggtgtaac ctttttcacg caacgcgttt tccagcacct ccagcggaac
    12121 gccacattgc gccgtggcct gcatattctc aatatcaata ttaatgattt gattcatggc
    12181 ggagccgtcg agcaccaccg agttttccac aacggtttcc agcccacctt cggtggcgga
    12241 agcaccggta cgtggcacac cgttaatttt gtgcgcattc ataaaattca gcacacggga
    12301 tacttgttct gtagaaccga gttttacgac cgctgccgga attggcaaag tataaatgcc
    12361 atgaatatcc ggaaatttac gaaaacggtc aatactgttc ttctttaata ctgtttcatc
    12421 ggtaattacg cgatctgcac caacaatttc ctttagctgg tcgacaatcg ctacgcgaga
    12481 taaagacata gtaattcctt cct
//
LOCUS       DAZFJL010000131        12313 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFJL010000131 DAZFJL010000000
VERSION     DAZFJL010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.133"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..569)
                     /locus_tag="ACHJ8C_003755"
     CDS             complement(<1..569)
                     /locus_tag="ACHJ8C_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5553540.1"
                     /translation="MTEKTLASPKPSEIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFVLLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIPIPIWILIILLLI"
     gene            complement(570..2069)
                     /locus_tag="ACHJ8C_003756"
     CDS             complement(570..2069)
                     /locus_tag="ACHJ8C_003756"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO5553541.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEKPDENVPAKLEVKNLSHPSLFKEV
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYHGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            complement(2130..3020)
                     /locus_tag="ACHJ8C_003757"
     CDS             complement(2130..3020)
                     /locus_tag="ACHJ8C_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO5553542.1"
                     /translation="MKNIKLLGSAMLVSLALFSQSSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLHGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDH
                     LNGKPVEKMIPVQPGVYTGK"
     gene            complement(3056..3910)
                     /locus_tag="ACHJ8C_003758"
     CDS             complement(3056..3910)
                     /locus_tag="ACHJ8C_003758"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HFO5553543.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFSKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQQVEIPLVLHGASGLSEQDV
                     QLCIRHGICKVNVATELKIAFVDALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
     gene            4252..5082
                     /locus_tag="ACHJ8C_003759"
     CDS             4252..5082
                     /locus_tag="ACHJ8C_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5553544.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            5114..6049
                     /locus_tag="ACHJ8C_003760"
     CDS             5114..6049
                     /locus_tag="ACHJ8C_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO5553545.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFARLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKHGTLHAQHVAGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            6142..6384
                     /gene="yhdT"
                     /locus_tag="ACHJ8C_003761"
     CDS             6142..6384
                     /gene="yhdT"
                     /locus_tag="ACHJ8C_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO5553546.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVSAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            6374..7825
                     /gene="panF"
                     /locus_tag="ACHJ8C_003762"
     CDS             6374..7825
                     /gene="panF"
                     /locus_tag="ACHJ8C_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO5553547.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVDTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            7837..8718
                     /gene="prmA"
                     /locus_tag="ACHJ8C_003763"
     CDS             7837..8718
                     /gene="prmA"
                     /locus_tag="ACHJ8C_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO5553548.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            9047..10012
                     /gene="dusB"
                     /locus_tag="ACHJ8C_003764"
     CDS             9047..10012
                     /gene="dusB"
                     /locus_tag="ACHJ8C_003764"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO5553549.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCVHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            10038..10334
                     /gene="fis"
                     /locus_tag="ACHJ8C_003765"
     CDS             10038..10334
                     /gene="fis"
                     /locus_tag="ACHJ8C_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO5553550.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            10420..11304
                     /gene="yhdJ"
                     /locus_tag="ACHJ8C_003766"
     CDS             10420..11304
                     /gene="yhdJ"
                     /locus_tag="ACHJ8C_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO5553551.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLITK"
     gene            11388..11567
                     /gene="yhdU"
                     /locus_tag="ACHJ8C_003767"
     CDS             11388..11567
                     /gene="yhdU"
                     /locus_tag="ACHJ8C_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO5553552.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(11570..12232)
                     /gene="acrS"
                     /locus_tag="ACHJ8C_003768"
     CDS             complement(11570..12232)
                     /gene="acrS"
                     /locus_tag="ACHJ8C_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO5553553.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHKTNELS
                     VI"
BASE COUNT         3179 a         2976 c         2872 g         3286 t
ORIGIN      
        1 tttatcagca ataagatgat taatatccat ataggaatag gtatatagag aaattctcca
       61 ttccccagcg ccagaaaagc atcatcttta atcatgatgg gcataccatc ggtcactata
      121 taagcgatac cacggaagat ccccatagta gcaacggtga caataaacga agggatcccc
      181 gctatagccg tcaagccacc atttatcatc cccagagcca gtgctgcaat aatggataat
      241 gggaccaata aaacaaatgg aattccccat gaagccccaa gcgcagcaaa tgtgccaatc
      301 aatgcaatgg ttgaaccgac cgataaatca atatcgccaa gcaaaagcac ataagtcatt
      361 ccataggcgg taattgcaat tatcgaaacc tgaagaataa tattagtaat gttattggcg
      421 gtaaaaaaac tgtcattaat aaaactgaac agaatgatta acaccaccat tccactgagg
      481 ataccaccat attgcctgaa gactttttta aaattatttg caatgggaat ttccgaaggt
      541 ttcggactgg caagagtttt ctctgtcatt taacgtactc ctgttgcagc ggtcattacc
      601 tgtgtctgcg ttaaattttc acatgaataa atatcggcaa tcgttttatt acgcattacc
      661 accagacgat cgctaagtgc catgacttct ggcagttcag aggacaccag aataattgct
      721 gtccctgccg cagccagttg acgtatcacg ttatagattt caaattttgc gccgacatcc
      781 actccgcgcg ttggctcatc gagcaataaa atacgcggag ttttctccag ccacttcgat
      841 aacacgattt tctgttgatt accgccgctc agtgctccca ctgcctgatg cacagaactc
      901 actttagtgt tcattctggc aattgattgc agcgtcttcg cctcttcttg tcggtgattg
      961 atgagtccca tccgcgaagc gttatattga aacgccacat ggtgcgtgtt catattgacg
     1021 tcgtgcatca gcactaaccc ttcttccttg cggttttcgg tcacaaaagc aatgccatga
     1081 tcaatagcct gtttgggatt agcgatcctg acttccttac catcaatcaa cacagtcccg
     1141 tgatacgaaa cgaggccaaa tagcgccttc atcacatcgg aacgcccggc ccccaccagg
     1201 ccaaaaaatc cgaggacttc ccccggacgc acagcgaaac tgacctcttt aaaaagcgaa
     1261 ggatgggaaa gattttttac ttccagtttt gcaggaacat tttcatccgg tttttcccca
     1321 agacgtgctg gccaaatatt tttcatttcc cgaccaacca tttgggcaat caagtgctgg
     1381 atatcactgt ttttcttttc atgcgtagcg atatactcac cgtcgcggat caccgaaata
     1441 tcatctgaaa tccgcataat ttcatccatg cgatgagaga tataaataac cgcacagcct
     1501 cgctgcttta atttttcaat aatgttaaac agaatctccg cttcattatc acttagcgag
     1561 gatgtcggtt catccataat cacgacatcc gctgcatagc ttaaggcttt ggcaatttct
     1621 accagttgcc tatgtgccat cgacatattg ccgaccattt ccagagggtc tagtgggatg
     1681 cccagttccg cgaggaggtt ctggcaatct gcgagcattt ttttatcgtt aatgatgcca
     1741 aaacgtcgcg gttcattatt ggcataaata ttttccgcca cggtcatatt attactcagg
     1801 ctcagttcct gaaaaataat cgcaatccct aactggcgag aatgctgcgg attattaatg
     1861 gtgatggttt caccacgtag tgttatcgtt ccctgggttg gcggataaac accggccagt
     1921 attttcatta atgtcgattt accggcacca ttctcgccta ataaagtatg cacccgccct
     1981 ttacgtaaag agagtgtaat atcttttaat gcctgaacat gaccaaatgt cttcgcaata
     2041 tgcgaaacat caagaataac atccgtcata atgacctcgg tgtcacaaca aaacgccaat
     2101 aacgttttgt tgtgaggtga agatatttac tatttacctg tataaacgcc gggttgtacc
     2161 ggaatcattt tttctaccgg tttaccattg aggtgatcaa cagcggcatc aatggcttta
     2221 gcccccattt gatcgggata ttgccgaatc acggccgcaa acgttttttc actgtcaaca
     2281 gcatcacgcg cttctttcat gccgtcaaaa ccaatgattt tgatgttgtc gttatgcgca
     2341 gattttgctg cgataaccgc tgccagcgcc gcatcatcac caaagccaaa cagaccattc
     2401 aggttggcat ttgcctgcat aatgttttgc gcagtcgtta acgcttcagc ccgcgtaata
     2461 ccaggctgtt cagcaacaat tttaatatcc ggtgtatcgc ttaacccttt cttaaaacca
     2521 tcaacgcgcg caaccaccga ttgtagtgcc ggataagaaa taatggctac agtaccttta
     2581 ccatgcaaaa gtttacccat taacttgcct gcttcaacgc ctcccgcata gttatcggtc
     2641 gcaacatgcg atacgaccgg aacgccttcc gcagcgacgt cgacggtgat aacgggaata
     2701 ccggccttat cggctttaat tatcgctgcc tggacgcctt tcgaatcaac cggagagata
     2761 ataatggcat ccactttttt agtaataaaa tcctcaacat cagaaagttg tttgtttaaa
     2821 tcctggttgg caatagagac acttaaattg acattgtctt tttttgcctc ttctttcatg
     2881 gcattcgcaa gatcaatata gaatggatgt tgttgtgtta ataatgacgc accaatttta
     2941 tactcttttg ccaacgaact ttgcgagaac aatgccaggc tcaccaacat agctgaacca
     3001 agtaatttaa tatttttcat aaacattcct cattgttttt gtagggtaca acgtattaaa
     3061 tttttccttc actaccacag acgccaataa tacgagttac gacttctttc atggcggcct
     3121 ttgcgggctg catatagtgg cgaggatcat ttgccgcagg attgttgtag aaatatcctt
     3181 ttaacgcatc gacaaacgca attttcaatt ctgtcgcgac atttacttta caaataccgt
     3241 ggcgaataca gagctgaacg tcctgttccg ataagcccga agcaccatgc aacaccagcg
     3301 gaatctccac ttgctgacgg atctgttcca gccgcacaaa atccagtcgt ggttcatgcg
     3361 catataaacc atgtgctgtg ccgattgcta ccgccaggga gtcaatttgg gtacgttgaa
     3421 caaactctgc agctgcggct ggtgaggtat aagcggaatc tttttcatcc accagcagat
     3481 cgtcctcctg gccgccaaga cgccctagct cggcctcaac gctaacatca aagcgatggc
     3541 aaaaggtggt aacttcttgc actcgcgcaa tgttgtcttc aaatggcaaa tgggagccat
     3601 caatcatgac agaacggatc cctgcgcgaa ccttgctaaa aatgtcatca ctctcttcat
     3661 gatggtcgag atgcaggact aacggtaggt tgtactcgcg agccagtgat tgtactaacg
     3721 aaatcaaact tcccacgccg ccataacgat aagtacccgg tgttcccgcc agcataacgg
     3781 gagagcgaag ttgtgcagcg ctctcaacaa ctacctgtaa agtttcaagg ttatgaatgt
     3841 tgaaagcagg gacggcatat ccctctgact gggctttttt cagcatgttt ttgcttgaaa
     3901 tgatgtacat cgctgctcct gttatttgtc gttcgcctta caaaatgaag aaagagaatc
     3961 ttcactttca ttttgaaagc aaactagcac agcaaaagcg gcaaaaagcg tgatgcatgt
     4021 cgaatatcat caaaataact tacaaaaaat aacttaattt gttttttact ttgcaagtga
     4081 aaccaatgac acctctgcat aaatatgaat atattcattt atttttcaat gtattaagct
     4141 attttcgttg cgatttttga cccatgattt tcttttcgaa ttgtcgatat tattgaagga
     4201 taactttcaa agaaaaatac aaggggctat agtgttacgc attaaaggcc aatgaagagt
     4261 gttaacgtgc cgagaaaatc gtctaaccta ctgaaacgca gattaaaaat tgcagaaatt
     4321 gttgccgccc agggtgaaat caaagttgat gacttatctg cgcaacttgg tgtctctggc
     4381 gtgacgatcc gcggcgatct cagttatctg gagcaacagg gatatttaaa gcgatcattt
     4441 ggcggtgcta ttgcgacacc actctcctct gaaccagaag cgatacaacc gatcgcgcca
     4501 ttgtctctgg cacaacaaat ggaaatagca cgacattgcg cacggttaat tcgcgatcgt
     4561 gacacgctat ttcttggaca tgggacaatc tgccgtaaga tcattccatt gctcagcgaa
     4621 gtgaaaaaac tgcgtctgat caccaacgat cccgaacatg cactgctggc aaatcagttt
     4681 attgatggcg aaataatact ggctggcagt gaaatgctgc gccccgacac cgcactggcc
     4741 ggaaaaccgc ttgaacaggc acttcagcat ttcaccatta cacatagcat tctggaaatc
     4801 agccatctgg atgctgatgg tactttaaat atcaacgttc ccagactcgc cgctgcctgg
     4861 cagctctgtt tcgatcatgc ccagaataag acggttatcg ttgccgccga tcctgcgcca
     4921 tcaacggctg ctatcgggca tttgcatcag gcagaaaatg tgatcgtcag ccgcagcatt
     4981 aacgaacaat atcagcaaca attgcagtcg gctgatttcg ttatccttta taccagtaat
     5041 gagtgcttta cctggtccaa tcgcgaaagg cttcaggaat gagaggggtt ttgtgacaac
     5101 aactatctgc gctatgggcg aattgctggc cgagtttttg tcccgcaacc cacatcaaaa
     5161 attcactcag cctggggagt ttatcgggcc atttcccagc ggtgcgccag caatttttgc
     5221 tgctcaggtg gcaaaactgt cccatcgggc catcttcttt ggatgtgttg gtaatgatga
     5281 ttttgcccga ctcattatag agcgtctccg tcatgaaggt gtcattaccg atgggatcca
     5341 tgttatgaac aatgccgtca caggtacggc gtttgtgagt tatcaaaatc cccagcagcg
     5401 ggatttcgtc tttaatatcc ctaacagcgc ctgcggtttg tttactgccg agcacattga
     5461 taaggatctg cttaaacagt gtaaccatct gcatattgtg ggctcatcgt tgttctcatt
     5521 tcgcatgatc gatgtcatgc gtaaagcaat aacgacgatc aaatcggctg gcggcaccgt
     5581 ttctttcgat cccaatattc gcaaagagat gctgagcatt cctgaaatgg cgcaggctct
     5641 cgattatttg attgaatata cggatatttt tatccccagc gaaagcgaac tccctttctt
     5701 cgcgcgtcac aaaaatctgt cagaggaaca gattgttagc gatcttctcc acggcggcgt
     5761 aaaacatgtg gcgataaaac gcgcccagcg tggggccagc tattacaagc ttaaacacgg
     5821 tacattacac gcccagcatg ttgcaggtca cgatatcgaa attatcgatc caacgggtgc
     5881 aggcgactgc tttggcgcaa cgtttatcac tcttttctta tccggtttcc cggcacacaa
     5941 ggcgctgcaa tatgcaaatg ccagcggcgc gctcgccgta atgcggcaag gtccgatgga
     6001 agggatatcc tcactggcag acattgaaga ctttttgcag cagcactgaa cggcaatttt
     6061 ctgtcagatc attttccctg cctgaagact tgccgtagaa ttgcccgttt ctcgtatccc
     6121 cccatgagga ggaccccttc cgtggacact cgttttgttc aggcccataa agaggcgcgc
     6181 tgggcgctgg ggctgaccct tttgtatctg gcggtatggt tagtatccgc ttacttacct
     6241 ggcgttgccc ccggttttac cggctttccg cgctggtttg agatggcctg catcttgacg
     6301 ccgctgctgt ttattggact gtgctgggcg atggtgaaat ttatctatcg cgatatccca
     6361 ctggaggatg acgatgcagc ttgaagtaat tctaccgctg gtcgcctatc tggtggtggt
     6421 gttcggtatc tctgtttatg cgatgcgtaa acggagcact ggcaccttcc ttaatgagta
     6481 tttcctcggc agccgctcta tgggcggtat tgtgctggcg atgacgctca ccgcgaccta
     6541 tatcagtgcc agttcgttta tcggcgggcc tggagctgct tataaatacg gcctgggctg
     6601 ggtattgcta gcgatgattc agcttcctgc agtctggctt tcgctcggta ttctcggcaa
     6661 gaagttcgca attcttgcgc gccgctacaa tgcggtgacg ctgaacgata tgctgtttgc
     6721 ccgctaccag agtcgtcttc tggtatggct ggcgagtttg agtttgctgg ttgcgttcgt
     6781 tggtgcgatg accgtgcagt ttatcggcgg tgcgcgcctg ctggaaaccg cggcgggtat
     6841 tccttatgaa accgggctgc tgattttcgg tatcagcatt gcgttgtata ccgcctttgg
     6901 cggctttcgc gccagcgtgc tgaacgacac catgcaaggg ctggtgatgc tgattggcac
     6961 cgttgtgctg cttattggcg tggtacatgc cgcaggtggt ttaagtaatg cggtagacac
     7021 gctgcaaacc atcgatccgc aactggttac gccacaaggc gctgacgata ttctgtcgcc
     7081 tgcctttatg acgtcgttct gggtactggt gtgctttggc gttataggcc tgccgcatac
     7141 tgcggtgcgc tgtatctctt ataaagacag caaagccgta catcggggga tcattatcgg
     7201 tacgattgta gtcgcaatcc tgatgttcgg tatgcatctg gccggagcgt taggtcgggc
     7261 ggtgatcccc gatctcaccg taccggatct ggtgatcccg acgttaatgg taaaagtgct
     7321 gccaccgttt gccgccggga tcttcctggc tgcaccgatg gctgcgatca tgtcgacaat
     7381 taacgcccaa ctgctgcaaa gttccgctac gatcattaaa gatctctatc tgaatatccg
     7441 tccggatcaa atgcaaaacg agacgcgtct gaagcggatg tcggcggtga tcacgttggt
     7501 tctcggtgcg ttgctgctgc ttgccgcctg gaagccgcca gaaatgatca tctggctgaa
     7561 tttgctggcc ttcggtgggc tggaagccgt tttcctgtgg ccgctggtgc tgggtcttta
     7621 ctgggaacgt gccaacgcca aaggcgcgct aagtgcgatg atcgttggcg gcgtgctgta
     7681 tgccgtactc gcgacgctga atattcagta cctgggcttc caccctatcg tgccctcgtt
     7741 actactaagt ttgctggctt tcctggtcgg aaaccgtttc ggtacatccg tcccgcaagc
     7801 taccgttttg actactgata aataaagagt tttgccatgc cttggatcca actgaaactg
     7861 aacaccaccg gcgcaaacgc ggaagatctt agcgatgcgc tgatggaagc gggtgccgtt
     7921 tctatcactt ttcaggatac ccacgatacg ccagtatttg agccgctgcc gggcgaaacg
     7981 cgcctgtggg gcgacaccga tgtgattggt ctgttcgacg ctgaaaccga tatgaacgac
     8041 gtggtggcga ttctggaaaa ccatccgctg ctcggcgcgg gcttcgcgca taaaatcgaa
     8101 caactggaag ataaagactg ggagcgcgaa tggatggata atttccaccc gatgcgcttt
     8161 ggtgaacggc tgtggatctg cccaagctgg cgtgatgtgc cggacgaaaa cgccgtcaac
     8221 gtgatgttag acccagggct ggcgtttggt acgggtaccc atccaactac ctctctgtgc
     8281 ctgcaatggc tcgacagcct cgatttaacc ggtaaaacag tcatcgactt tggctgcggt
     8341 tccggcattc tggctatcgc ggcgctgaaa ctgggtgcag caaaagccat tggtattgat
     8401 atcgatccgc aggcgatcca ggccagccgc gataacgccg aacgtaatgg cgtttctgat
     8461 cgtctggaac tctacttacc gaaagatcag ccagaagaaa tgaaagccga cgtggtggtc
     8521 gctaacatcc ttgcaggccc attacgtgaa ctggcaccgt taatcagcgt cctgccagtt
     8581 tcaggcggtt tgctgggcct ttccggtatt ctggcaagcc aggcagagag cgtttgcgaa
     8641 gcttatgccg acagcttcgc tctggacccg gtcgtggaaa aagaagagtg gtgccgcatt
     8701 accggtcgta agaattaacc ttcgcatcgc cgtatggcga cgcagagcga agtgcgagcg
     8761 atgtcacaaa aggcacgtta atttgccgat tatttacgca aatttgcgtg ccaaaatttt
     8821 cattcataaa gaaaaattga gaacttactc aaatttcttt gagtgtaaat tttagtcact
     8881 actttctaat atgatgattt ttatgagtaa ttatcgcgct acgctcattt taaatgcaat
     8941 tctttgatcc atctcagagg attggtcaaa gtttggcctt tcatctcgtg caaaaaatgc
     9001 gtaatatacg ccgccttgca gtcacagtat ggtcatttct taactcatgc gcatcggaca
     9061 atatcagctc agaaatcgcc tgatcgcagc gcccatggct ggcattacag acagaccttt
     9121 tcggacgttg tgctacgaga tgggagccgg attgacagta tccgagatga tgtcttctaa
     9181 cccacaggtt tgggaaagcg acaaatctcg tttacggatg gtgcacattg atgaacccgg
     9241 tattcgcacc gtgcaaattg ctggtagcga tccgaaagaa atggcagatg cagcacgtat
     9301 taacgtggaa agcggtgccc agattattga tatcaatatg ggttgcccgg ctaaaaaagt
     9361 gaatcgcaag ctcgcaggtt cagccctctt gcagtacccg gatgtcgtta aatcgatcct
     9421 taccgaggtc gtcaatgcag tggacgttcc tgttaccctg aaaattcgca ctggctgggc
     9481 accggaacac cgtaactgcg aagagattgc ccaactggct gaagactgtg gcattcaggc
     9541 tctgaccatt catggccgta cacgcgcctg tttgttcaat ggagaagctg agtacgacag
     9601 tattcgggca gttaagcaga aagtttccat tccggttatc gcgaatggtg acattactga
     9661 cccgcttaaa gccagagctg tgctcgacta tacaggggcg gatgccctga tgataggccg
     9721 cgcagctcag ggaagaccct ggatctttcg ggaaatccag cattatctgg acactgggga
     9781 gttgctgccc ccgctgcctt tggcagaggt taagcgcttg ctttgcgtgc acgttcggga
     9841 actgcatgac ttttatggtc cggcaaaagg gtaccgaatt gcacgtaaac acgtttcctg
     9901 gtatctccag gaacacgctc caaatgacca gtttcggcgc acattcaacg ccattgagga
     9961 tgccagcgaa cagctggagg cgttggaggc atacttcgaa aattttgcgt aaacagaaat
    10021 aaagagctga cagaactatg ttcgaacaac gcgtaaattc tgacgtactg accgtttcta
    10081 ccgttaactc tcaggatcag gtaacccaaa aacccctgcg tgactcggtt aaacaggcac
    10141 tgaagaacta ttttgctcaa ctgaatggtc aggatgtgaa tgacctctat gagctggtac
    10201 tggctgaagt agaacagccc ctgttggaca tggtgatgca atacacccgt ggtaaccaga
    10261 cccgtgctgc tctgatgatg ggcatcaacc gtggtacgct gcgtaaaaaa ttgaaaaaat
    10321 acggcatgaa ctaattcagg ttagctaaat gcttgattaa aaaggcgcta ctcggcatgg
    10381 ggaagcgcct tttttatagg tgtcacaaag ggagtgacca tgagaacagg atgtgaaccg
    10441 acccgatttg gtaatgaagc aaagaccatc atccagggtg atgcattaac cgaacttaaa
    10501 aagctccccg ccgaaagtgt cgatctgatc tttgccgacc caccgtataa catcggtaaa
    10561 aattttgatg gtctgatcga agcctggaaa gaagatctgt ttatcgactg gctgtttgaa
    10621 gtgattgcag agtgccatcg cgttctgaaa aagcagggca gcatgtacat catgaatagc
    10681 acggaaaaca tgccctttat cgatctccag tgccgcaagc tttttaccat caaaagtcgc
    10741 atcgtctggt catatgacag ttctggagtg caggcgaaaa aacactacgg ctccatgtac
    10801 gaacccatcc tgatgatggt gaaagacgca aagaactaca cgttcaacgg tgatgctatt
    10861 ctggtagaag ccaaaaccgg atcgcagcgc gcgttgatcg attatcgcaa aaatcctcca
    10921 cagccttaca atcatcaaaa agtaccgggt aacgtctggg attttcctcg cgtgcgttat
    10981 ttgatggatg aatatgaaaa ccacccgacg caaaaaccgg aagccttact gaaacgcatt
    11041 attctcgcct cttccaaccc aggcgatatc gttctcgacc cgtttgccgg tagctttact
    11101 accggtgccg tagccatcgc cagcgggcga aaattcattg gtattgagat caacagcgag
    11161 tacatcaaaa tggggcttcg acggctggat gtcgcgtcgc attactctgc ggaagaactg
    11221 gcgaaagtga aaaaaagaaa gaccggcaac ctgtcaaaac gaagccggtt aagcgaagtt
    11281 gaccccgatc tcattacaaa gtaaaggaag tgtaagcctg gttttcagat tattcatttc
    11341 gtgtatattt cctgccagac ttggttaaac atgcacaggc tcaggtaatg attcgcaagt
    11401 attggtggct cgtcgttttc gctgtcttcg tttttctgtt tgatacttta ctgatgcagt
    11461 ggattgaact gctggcaaca gaaacagaca aatgccgcaa tatgaactca gttaatccac
    11521 taaaactggt taactgtgac gaactgaatt ttcaggacag aatgtgaatt tatatgacac
    11581 ttaattcatt cgttttatga attaatttcg ttatgttttc atctggcatg aacattctta
    11641 atacgttatc gaccagagcg ggggcttgtt tataaagatc ataacccgcc atattcatta
    11701 accagttttg aacaattccg ctgaaggcac catcaataat aatcatcaca acatctaaat
    11761 cgagattatt tgctacacaa ccttgttgct gacacgcctg caatacttcg cggagagtct
    11821 gcggattaaa gcccatcttt tcgcgtatca ctccctcggc cagcatctca tcattaaatt
    11881 cacatttgtg atataagatt ttcagcaacg cctgctggcg gggaattttg gcaatatatt
    11941 gcaaaccgac aatcaatttt tcacgcaatt gctgaaacgg gtcatgctct aacccagcgg
    12001 tcaagtgatc ctggattaac tcccgcaatg aaggctgttg caaccacatc tcattaaaca
    12061 gttgagtctt gttttcgaag tgccagtaga tagcgccacg cgtaacgtta gcagcgtcgg
    12121 caatgtcgtt gagcgtcgtc ttgcttacgc catgctgcgc aaactgggcg atggcagttt
    12181 caatcagttc ttgccgggtc ttcagggctt cagctttggt tctttttgcc atgattaatt
    12241 attcaggaaa taaatatatt cgacacagga tgagaaaata gcgaaggtta atctatcacc
    12301 taatgtgtat tta
//
LOCUS       DAZFJL010000132        12242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFJL010000132 DAZFJL010000000
VERSION     DAZFJL010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.134"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..411)
                     /gene="yhaJ"
                     /locus_tag="ACHJ8C_003769"
     CDS             complement(<1..411)
                     /gene="yhaJ"
                     /locus_tag="ACHJ8C_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO5553554.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGA"
     gene            516..1217
                     /gene="yhaK"
                     /locus_tag="ACHJ8C_003770"
     CDS             516..1217
                     /gene="yhaK"
                     /locus_tag="ACHJ8C_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO5553555.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASASEVLLLSTQPGVSYS
                     EHNLSKDKPLTRMQIWLDACPQRENPLIQKLALNMSKQQLIASPEGGMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1240..1404
                     /gene="yhaL"
                     /locus_tag="ACHJ8C_003771"
     CDS             1240..1404
                     /gene="yhaL"
                     /locus_tag="ACHJ8C_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO5553556.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1537..2847)
                     /gene="cyuA"
                     /locus_tag="ACHJ8C_003772"
     CDS             complement(1537..2847)
                     /gene="cyuA"
                     /locus_tag="ACHJ8C_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO5553557.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AISDAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVTEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGNWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(2875..4206)
                     /gene="cyuP"
                     /locus_tag="ACHJ8C_003773"
     CDS             complement(2875..4206)
                     /gene="cyuP"
                     /locus_tag="ACHJ8C_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5553558.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKIPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4481..5845)
                     /gene="tdcG"
                     /locus_tag="ACHJ8C_003774"
     CDS             complement(4481..5845)
                     /gene="tdcG"
                     /locus_tag="ACHJ8C_003774"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO5553559.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(5917..6306)
                     /gene="tdcF"
                     /locus_tag="ACHJ8C_003775"
     CDS             complement(5917..6306)
                     /gene="tdcF"
                     /locus_tag="ACHJ8C_003775"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO5553560.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6320..8614)
                     /gene="tdcE"
                     /locus_tag="ACHJ8C_003776"
     CDS             complement(6320..8614)
                     /gene="tdcE"
                     /locus_tag="ACHJ8C_003776"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO5553561.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPLDFDTNIATTITAHDAGYIDQPLEKIV
                     GLQTDAPLKRALHPFGGINMIRSSFHAYGREMDSEFEYIFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRRALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGMDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPIRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(8648..9856)
                     /gene="tdcD"
                     /locus_tag="ACHJ8C_003777"
     CDS             complement(8648..9856)
                     /gene="tdcD"
                     /locus_tag="ACHJ8C_003777"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO5553562.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLNASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(9882..11213)
                     /gene="tdcC"
                     /locus_tag="ACHJ8C_003778"
     CDS             complement(9882..11213)
                     /gene="tdcC"
                     /locus_tag="ACHJ8C_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO5553563.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11235..12224)
                     /gene="tdcB"
                     /locus_tag="ACHJ8C_003779"
     CDS             complement(11235..12224)
                     /gene="tdcB"
                     /locus_tag="ACHJ8C_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO5553564.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
BASE COUNT         3138 a         3330 c         2922 g         2852 t
ORIGIN      
        1 cgcccccgcc agcacttcgg tgatgattgc cagttgggta ttggcttttg ccgccagttt
       61 gtcgattaac gggaaaaagg caggtgtcgg taccagcgcc tcggtcacaa tggtgagatg
      121 cgtttcccaa ccacgcgcga gggcttccgc atcggtagtc agtttatctg cggcttccag
      181 caaaacgcgc cctcgttcca gcaacatcct cccgacattg gtgaatttgg tgcgatggcc
      241 cgagcggtca aacagcacca catccagctc ttcttccagt ttttgcatgg tgtagctaag
      301 tgcggaaggc acgcgtccca gctcatccgc cgccgccgca aaactgcccc ggcgatcgat
      361 cgcatccata acccgtagtg cttccagcgt taatgccctt tctttggcca tttcgttctc
      421 attcaggaaa tttgaacata ccgggcagaa tatctggcta acaatgtagc gtccagcccc
      481 ctaccataaa aggaagtaaa gagaggtcaa gaattatgat tactacccga actgccaggc
      541 agtgtggaca agcagactac ggatggttgc aggcccggta tactttttcc tttggacact
      601 acttcgaccc gaaattgtta ggctatgcct ccctgcgcgt gcttaaccag gaagtgctgg
      661 ccccaggtgc cgcctttcag ccgcgaacct atcccaaagt cgatatttta aatgtgattc
      721 tggatggaga agctgagtat cgcgacagcg aaggcaatca tgttcaggcc agcgccagtg
      781 aggtgttgct gctctctacc cagccgggtg tcagctatag cgaacacaat ctcagcaaag
      841 acaaaccgtt aacgcgaatg cagatttggc tggatgcctg cccgcagcga gagaatccgc
      901 tgattcaaaa gctggcgctt aatatgagca agcagcaatt aatcgcctcg ccagaaggtg
      961 ggatgggaag cctgcaattg cgccagcaag tgtggctgca ccatatcgtg ctcgacaaag
     1021 gcgaaagtgc gaatttccag ttgcatgggc cacgcgcgta tttgcaatcg attcacggga
     1081 aatttcatgc gcttacgcat catgaagaga aagcggcgct aacctgcggt gatggggcgt
     1141 ttattcgtga cgaggctaac attacgctgg ttgccgattc cccactgcgc gctttgctga
     1201 tagatttgcc tgtctagttg tttttacagg gagatgatga tgagtaaaaa atcggccaaa
     1261 aagcgtcagc cagtgaagcc cgtggtggcg aaagaacctg ctcgcaccgc caaaaatttt
     1321 ggctatgaag agatgttgag cgagctggaa gccatcgtcg cggatgctga aacgcgttta
     1381 gccgaggatg aagctaccgc gtaaagacag gtgccggatg cggcgtaaac gccttatccg
     1441 gcctacaaaa cccaacaaca tcaacaaatt gtgcaattcg taggccagat aagacgcgcc
     1501 agcatcgcat ccggcacaat acccaaaacc tggcgtttat ctggccttgc tcgccataat
     1561 ctcgataatc tgccgatccg tttgctgcat cgaatggctt gctaacgcac acaggttggc
     1621 aatcgactgc tcaacatcat gcgccacaat cccttcattg ccggtcacgg cggtatcatc
     1681 cagcgccatt aacaccgctt tccacgcagc cgaagcactg gtcgaaacct tcatcgcgca
     1741 gctgttcgat gcaccatcgc aaatcatgcc actgacatcg ccgatcatac tgctgatcgc
     1801 catcgagatg gtttcataac gcccatccac cagccatgcc atcccggcag cggcccccat
     1861 tgctgcggtc gttgcggcac acagcgcaga caaacgcggt aactggttat ggatgtaaat
     1921 cgcgctcaaa tgcgaaagca tcagcgcacg cgccagccgt tcatcatccg ctccgaagtg
     1981 ttctgctacc accaccacgg gcattgttgc ggtgatcccc tggttacccg agccggagtt
     2041 actcattgcc ggaagcgtag cgccgcccat acgcgcatcg gatgccgcgc tggtacgaat
     2101 cacaatggat gaagagagat ctttcgccag caaaccgcgc gcgcactgtt tttccagcgt
     2161 cgcaccaata tgcagccccc agttaccgct caaaccttcc tgcgataacg cacaatttaa
     2221 cttcgcagaa tcgagaataa agcggatcgc cgcaaacggg acttcattga cgaacttcag
     2281 gatctcagcc agcgtcgtcc tggaaagcac cgtcagcggc gactcttgct cgccctctgt
     2341 cacacacgcc tgctgggtaa acaccacacc attgtgcgtc tcaatatgca caatgttggt
     2401 atgcccgccg acgatggtga cacacgccca cttctcaccg ttccagactt tggcgcgtga
     2461 gaagaggatt tcatcgcaag gttcctggat cttaactgag actttccccg ccgccagcag
     2521 tgctttggca tcggaaattg cctgcgcagt tgcgtctttc agcacttcca gcccggcgtt
     2581 agcatttcca cctaacgctc ccagcgccgc cgcaattggc agccccacca ttcctgtacc
     2641 gggaacggtg acacccagac cattcttcat cagatttggc gaaacccagg cttctacacg
     2701 ttcaaccgga ccttccagtt cagccgcagc aaccgccgcc gccagcgcca gtgaaatcgg
     2761 ttcagtacac cccagcgccg gttttacttc ctcctgaacg gcgaggatgt aacgctgcca
     2821 taacggattt aaagtcgaat caaacatatt aaaaacctta aaatttcagg taaatcaaga
     2881 aaatgccagg aacggagaaa cacaaagcaa caaaccagtg acgataatca ggtacagaga
     2941 catccctttg tatttgtgca atgccggtac tttgtaaacc agccacgccg ggatcaggca
     3001 ccctaccatg ccgaaaatcg ggctacagat agaggtgaag ctcaacaccg gggcgttcag
     3061 tacgatggcg ctccaggcca gcaaaatggc gaaaatcatg atgccgcgct gaacgagatt
     3121 ttcgttaatc ttctcggcag gtatcttgcg acgcaggatg ttcattacga tcccttgcgt
     3181 tgcttcgcga aagcctaaat agacgccaaa gaatgccgtc attacagcaa agatattgag
     3241 aatgacgctg accactttca cccacgctgc gccgtcaccg ctaataaact gtgcggcaat
     3301 cgccagcgcg gaaatattct gctcgtaggc ttttaccgct tcgtcatgtc ccatcgccag
     3361 cgtgaatgac acggcgtaga aaaagacggt gacaaacaaa atgccaaacg cgatattcat
     3421 tgcccgcaat gctttatgcc gcgccacttc aattgatttt tcccgcgaac gataagagat
     3481 caccatcggg cttaacgtct ggataaacag aatcgacgtc agggtaaacg gcagcgtaat
     3541 aatggcattt ttcaccagca gccccagcgg cggtagcgaa ccgacgttat acagatgcca
     3601 cattcccacc atcgacacgc ccagcgccgc gaccaccagc agcttggtca gcaccatgcc
     3661 ggtcgaaatt ttgaataaca atttctcgcc gcgtgaggag atcgccacca gaatgcaaat
     3721 cagtaccaga ccataaaagg gactgtctga cagcaaccct tccgtcacgc cgaaggtatg
     3781 caggtaggaa gcactatcgt tggtgatggc ggtggaatag acgaacatcc aaatcaccag
     3841 cattacgaaa tagagtgcac ctaacaggat gccccagttt ttacctaaat aaccgctgat
     3901 gacgctcggg taatctttac actccggtga ttctgccagc gtattaataa acaaccgctg
     3961 aaacagatac attgccgggt aaccaatcac cgatgagagc aaaaataccc acaatcccat
     4021 caaaccgacc tgcaccggga gaaaaacaat ccccgcgcca atcgccatcc cgatactcat
     4081 aatcacccag ccggtgtcag tactgtcgaa tttgatcgct tctcgccact cgctctcact
     4141 cattccagca cgacccgccg gggtcgaagc gtctgcaatg acgcctttat tcgatgcaat
     4201 ttccataatt tctcgctcaa tattttgtag ggcttattat ttttttccga gccgcatcaa
     4261 ggcgatatgc ggtctacgtt tttgcaggcg agttattaga ataaagaaat gatacgcgcg
     4321 aggtcagaga aaatcttcac aatcaagact ttgaaataac ggagatggat aagaattttc
     4381 tactaaatta atcgcaagag aatattcatt ctctatttgc ggcagatcac aaaaaaaagg
     4441 tgcacatttg tgcacccaag gatgaaagct gacagcaatg tcagccgcag accactttaa
     4501 tagccagtcc tccgcgtgat gtttcgcggt atttatcgtt catatctttg ccggtttcat
     4561 acatcgtctc gatcacttta tcgagtgaaa cacgcggtgc cgaggtgcgg cgcatcgcca
     4621 tccgcgcggc gtttactgct ttcacggcat taatggcatt acgttcaatg cacgggattt
     4681 gtacctgtcc ggcaaccgga tcgcaggtca gcccaaggtt atgctccatc gcgatttccg
     4741 ccgcattgca tacctgcgcc ggactgccgc ccagtagttc agttaaccct gccgccgcca
     4801 ttgaacaggc cacgccaatc tccccctgac agccgacttc cgcgccagag atggaggcgt
     4861 tcattttata cagcgcgcca attgcccccg ctgccagaaa atagcgggca attgaccgct
     4921 cgtttaccgg acgacggaac ttatcgtaat aagccagcac tgccggaata atgccgcacg
     4981 caccgttagt cggtgccgtt accacgcgcc cgccagctgc gttttcttca ctaaccgcca
     5041 gcgcgtacat gttgatccag tcgatgacat tcatcggatc gttagagatg ttatcgctgg
     5101 aaaccagttg acgacgcagc gctacggcac ggcgcggcac attgagcgga ccaggcagca
     5161 cgccttcagt attcattcca cgttcaatac cgtcatgcat cacttgccag atacgggcaa
     5221 accctgcgtc aatttccgct ttactgcgca gcgccagctc gttgtgcatc atcaggccag
     5281 atatagacag gccgttgtaa tcacacattt tcagcagttc acctgctgag tggaaatcgt
     5341 aaggtacggg tgtttcgaca tcgtgcgaca ggccgaagtg ttcttcttcg acaataaacc
     5401 cgccgccgac agagtaatag gttttgctta atagctcttc ctgtcccttc caggcagtga
     5461 tccgcattcc gttctcatgg cgaggcaaca tttcgggatg gaagataatg ttctttgcta
     5521 caggaaaatc aacaatatgc gcgccagaag ccaccggcaa ccgcccgctg cgcgtcacta
     5581 actctataaa tgcagggatc tcatcaatga caacatcctg cggactgttt cctgccagtc
     5641 ccatgatgat ggcgacatcc gtggcatggc ctttgcccgt cagtgacaac gacccgtaca
     5701 gatcgaccac aatatggctc gtcgcggtta ataagccgct actttccagc cgatcaataa
     5761 aactttttcc ggcattcatt ggccccactg tatgcgaact ggagggacca atcccaattt
     5821 tgaaaatatc gaatgcacta atcatatcca caccctcgga ttgccgttca gtgaagtgga
     5881 gcggaacgac ctagcgaccg tcccgctcac gaggccttac gcactacgta ctgcgatggc
     5941 ttcaatttcc aacttcacat ctttcggcaa acgcgcgacc tgcacacagc tccgggtcgg
     6001 gtaggtcgcc tgatgttcat cgaagaactg cttatagact tcattgatgg tggcaaaatc
     6061 attcagatcg gtgatgaaca cggtcatctt gatgatatcg cccacactca gcccggcagc
     6121 aaccacgatc gctttgacgt tttcgaggct taaacgcgcc tgatcttgca catcagccgg
     6181 gatctcaccg gtctgtgggc aaaccggaat ttgcccggag gtgaagacca tgctgcctaa
     6241 atcaacgccc tgaacataag ggccgattgc gcctggggca cgttgcgttt cgataatctt
     6301 tttcatacat cctccggcgt cagagcgcct gggtaaaggt acgtgaaata acatcctgtt
     6361 gctgttcacg ggtcagtgcg ttgaagcgca cggcgtagcc agagacacgg attgtcaggt
     6421 taggatattt ttccgggtgc tcgatggcat ccagcagcat ttcccgattc attacgttga
     6481 cgttgaggtg ttgaccgcct tcgacatccg cttcgtggtg gaaataccca tccagcaggc
     6541 cgacaaggtt cgttttacgt atcggatctt ctttgcccag cgccgcaggg acgatggaga
     6601 aggtgtacga gatcccatct ttggcgtagg tgaatggcag tttcgccacc gacgtcaatg
     6661 aggctacggc gcctttgcga tcacgaccgt gcatcgggtt agcgcccggc gcgaacggtg
     6721 ttccggcgcg gcgaccgtcc ggcgtgttcc cggttttctg accgtacacc acgttagagg
     6781 tgatagtcag aatcgattgg gtcggcacag cattgcgata ggttggcaaa gctttaattt
     6841 tcttcataaa gcgttcaacc agatcgcagg caatgctgtc tacgcgctcg tcgttgttgc
     6901 catactgcgg atattcgccg tcaatctcga aatccaccgc caggccgttt tcatcacgga
     6961 ttggtttcac acgagcatat ttgatggcag acagggagtc cgttgccacc gacaggcccg
     7021 cgatgccgca cgccatggtg cgatagacat cacgatcatg tagcgccatc agcgaagctt
     7081 cgtagctgta cttgtcgtgc atgtagtgaa tgatattcag cgcgctgatg tactgtaccg
     7141 ccagccagtc catgaagtgg tcgaggctat ccatcacttt gtcgtagtcc agcacgtcat
     7201 ccatcaacgg agccgttttc gggccaacct gaatcttcag cttctcgtcc attccgccgt
     7261 tgattgcgta cagtagcgtt ttcgccaggt tagcgcgtgc tccaaagaac tgcatttgct
     7321 taccaatcac cattgggctg acacaacagg caatcgcgta atcgtcgcta ttgaagtcag
     7381 tacgcatcag atcgtcgttt tcatactgca aagaggatgt gacgatagac acctgcgcgg
     7441 catatttttt gaaggcaatc ggcaaggctt ccgaccacag aatagtcagg ttaggttccg
     7501 gtgcaggccc catagtgtgc agggtgtgta aatagcggaa ggagtttttg gtcaccaggg
     7561 ttcgaccgtc cagccccatc ccgccgatca cttccgtcgc ccagatcggg tcgccggaga
     7621 acagcgaatc aaattccggc gtacgcaaga agcgcaccat acggatcttc atgatgaagt
     7681 ggtcgatcag ttcctgtgcc tgctgctcat tgagtacgcc tgctttaaag tcgcgctcaa
     7741 tgtagatatc gaggaacgat gccgtgcgcc ccagcgacat cgcgccgcca ttttgcgatt
     7801 tcactgccgc caggtaagcg aagtagagcc actgcaccgc ttcctgtgca ttctgcgccg
     7861 gacgggagat gtcaaaaccg tatttcgccg ccatttcttg aatctgcaac agcgcacgac
     7921 gatgttccgc cagctcctca cgcagacgga tcgtagcttc gagatcttcg cctttttcca
     7981 gacgggactg gagatcggca aactgcagtt cgcgttcacg taccagataa ctgatgccgt
     8041 aaagcgccac gcggcggtag tcaccgatga tacgcccacg accataacca tctggtaaac
     8101 cggtcagtac accagattta cggcagcgca gcatatccgg tgagtaaaca tcaaatacgc
     8161 cctgattatg ggttttacgc agatcggtaa agatgtattc aaattcgctg tccatttcac
     8221 gaccatacgc gtggaatgaa cttctaatca tattaatgcc gccgaacgga tgcagcgcac
     8281 gctttaatgg cgcatccgtt tgcagaccca ctattttttc cagtggctgg tcaatatatc
     8341 ctgcatcatg agcggtaatt gtggtggcaa tattggtatc gaaatctaac ggcgcgtgag
     8401 tcgcattttc gatacggatg ccttccatga ctttttccca caattccgtg gtggcaggcg
     8461 tcgcttcggc gagaaaagat tcatcgcctt cataaggtgt atagttatgt tgaataaaat
     8521 cgcggacatt aatttcgttt ttccagtccg tacctttaaa gccaagccat gcgtcggcgt
     8581 acagcttatc gctggtatca atatctacct tcatgaaaaa taatctctct acaatacttc
     8641 aactaaatta tgcaaattct gcgggcgcgt tcactttgcc taaatgaatg gcatccaaag
     8701 caatcatttt ttcttcgtta gtcggaatta cggcgcaaat gacacgcgca ttttcactgg
     8761 aaacaattcg ctctccaaaa gagttagagc gattattcat ttctgtatca atctctacgc
     8821 ctaatacagc taaatgttcc atgaccagac gacgaattaa acttgaattc tctcctattc
     8881 cgccggtgaa tataattcca tccaggcgat gtaatgaagc agcgtgtccg gcaatatgac
     8941 gggcaattcg gtgaacaaag gttttaattg ccagttgcgc gcgttcgtga ccttcatgcc
     9001 aggctttttc cagaacacgt aaatccgaag aaaggccgga aatacctaat aatcccgact
     9061 ctttattcac tacgcgttcc aggtccccca ggctctggtt ggtttggctg gcgacccagg
     9121 acatcgcccc gaagtcgaca tcaccactgc gggtacccat catcaagcct tccagcggcg
     9181 tcattcccat ggaagtgtca acactctgac cgttgcgaac cgcgcagatt gacgcgccat
     9241 tgccaagatg cgccacaacc aggccggagt catcttccgc cagattcagc agagaatgtg
     9301 cgcgctggga aacatagcgg tgcgacgtgc cgtggaaacc ataacggcgt acacctaact
     9361 cttcataata tttccacggc aggccgtata aataagcttc cggagccatc gtctggtgga
     9421 aactggtatc aaataccgcc acctgagtta cgcccgggaa taattgctgc gccgattcaa
     9481 taccacttaa attggcgtaa ttatgcaggg gggccagtgg agaaacgcga cggatattat
     9541 caatgacttc atcggtaata atggcggact cggtaaaaat actgccgccg tgagcgatgc
     9601 ggtggccaat taaggccaca ctgtcattta aattccgttt ttccagttca aatgcaattg
     9661 ccttcaatgc accttcgtag ctgtggtgag ccagcggtgc tggctctccc ccatttacgg
     9721 ataagaatgc attttccgag ttaataccgt cggcaatacc tgacattaat acttcacagt
     9781 cgctggcatt gagtacggaa aacttaatcg aagacgaacc acagttaata accaaaacaa
     9841 ccggaaattc attcatctct tttctcatcc tgagttacgg attaaaacag tttgtatacg
     9901 atgttcagga tggtcagcag accaatcacg gtaacaaaca cgttatccag acgaccacgg
     9961 tatttcgcca gagacggcgc tttacggatg gcatacatcg gcaacaggca cagcagtgat
    10021 gcgataatcg gtgcgcccat ggcttcaatc aggtcgagga tgttcgggtt ggcgtaggca
    10081 acaacccagg tggagcccat aatgaaaatc atgctgatag tattcagttt acccagcgac
    10141 actttggttt tgtcaccttt ataaccgaac ttcagaatca aaccattcag cccttccagc
    10201 gtccccagat agtgaccgaa gaaagatttg aaaatagcca cgagtgcgat gatggaagcg
    10261 gcatattcca gtgtaatcgc aaacgttgtt ttggtaccgg tcatggacgc aaagtggtta
    10321 gccagataag aaagcactgg aatattctgc gctttcgctt cagccatatt cgccggagac
    10381 agcgtaaaca ggcagctaaa ggcaaagaac atcaccactg caaccatcag catactggcg
    10441 cgagagatga tttgtgaaca tttacgctcg gtgaagtcgc gaccaaaatc tttctcgtac
    10501 tcttcacgtt tagaaaccac gaaggaagag acgattggcg agaagttaaa ggagaaaacc
    10561 atgatggaaa tccccagcca gacggtgatc aggataccgt catgaccagt taacgacagc
    10621 gaaccgaggt caacctggtc gataactgca gagttccagt aagggatcag cgaaagagaa
    10681 atcagcacca ggctggcgat aaacggccat accagatagc tcatcacttt aaccatcaga
    10741 tccttaccaa accagatgac gaaagccatc agcagcaaca ggaacagcgc cacaaagccg
    10801 cgattcagcg gtgcaaagcc gagctggttt tcccagaacg tcataaaggt attggtaata
    10861 gtaacgccat aaatccacaa cagtgggcaa atcgcgaaga agtacaggaa cgtgataacc
    10921 acgccgccag ttttaccaaa atgctcttcc accgtttccg taatgttgcc ggaagggtta
    10981 gagccagaaa gacacagacg cgccagtgcc cggtggcaat aaaacgcgat ggggtatgct
    11041 aataccaaca tcagaagaat cgggatcagt ccgccaaaac ctgcgcggat agggaagaac
    11101 agcaccccgg cgccgattgc cgtaccaaac aagcctaacg tccatgtggt atctgattta
    11161 cgccaggacg attgttttgt ctggctggat acaatgctat ctgaagtact catatcctat
    11221 cctcaacgaa ttaattaagc gtcaacgaaa ccggtgattt gagagacgcg agaaagatcg
    11281 atattgccgc cggaaataat actgacggtt tttctgtttt gaatatattg gtctaattta
    11341 ccgcttaata atgcagcaca tgccagagcg cctgcgcctt cggtgacgac tttattgcgc
    11401 tgaattaagg caatcatact gtttctgatt tcgtcttcgc tgaccagcac gatgtcatcg
    11461 actaattcac gaacgatttc gtaagttaaa ttacccgggc gggagacatc acaaccatcc
    11521 gccagggtgc cggtagttcg gtgcgtagtt atttctccgg agtggaaaga agccgccatg
    11581 ccgtgaacgt tttcagactg tacgccaata acacggatgg tcgggttgat agatttaatt
    11641 gccaccgcaa taccagcaat taaaccgccg ccgccaattg gcacaatcac gttatcgaca
    11701 tcatagagat cttccataat ttccagacca atcgttccct ggccagcaat cactttcgga
    11761 tcatcgtaag gtgggataaa aatacggcct tccatttcga caatttcgct cactttagca
    11821 atagtgtcgt tgaagttatc accatgcaaa acgacttctg cggagtagtc gcacgttgcc
    11881 gcgactttgg atttcggcgc accttttggc atcaccactt taccgtcaat acccagcatc
    11941 gcacaggaga gcgaaacccc ttgcgcatgg ttgcccgcag agcaggccac tacaccttta
    12001 cgtttttccg catcagtcag tgaacttaat ttattaaatg cgccacgaat tttaaatgaa
    12061 ccggtacgct gcatgttttc gaatttgagg aatatttcac ctttgcaacg ttcactaaaa
    12121 tagttggagc gaggcatacc tgttttataa attcgcccag ccagtcgttg ttttgcttca
    12181 atgatgtcat caatagcaac cgggagatcg tatgtaatat gcattataaa acctcttagc
    12241 ct
//
LOCUS       DAZFJL010000133        12180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFJL010000133 DAZFJL010000000
VERSION     DAZFJL010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.135"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            67..450
                     /locus_tag="ACHJ8C_003780"
     CDS             67..450
                     /locus_tag="ACHJ8C_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaA family stress response protein"
                     /protein_id="HFO5553565.1"
                     /translation="MSVLYIQIRRNQITVRDLESKREVSGDAAFSNQRLLIANFFVAE
                     KVLQDLVLQLHPRSTWHSFLPAKRMDIVVSALEMNEGGLSQVEERILHEVVAGATLMK
                     YRQFHIHAQSVVLSDSAVLAMFKQK"
     gene            complement(514..957)
                     /locus_tag="ACHJ8C_003781"
     CDS             complement(514..957)
                     /locus_tag="ACHJ8C_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO5553566.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVH
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTERSEVDDLGKPYPLLNLAYVGV"
     gene            1114..2043
                     /gene="metA"
                     /locus_tag="ACHJ8C_003782"
     CDS             1114..2043
                     /gene="metA"
                     /locus_tag="ACHJ8C_003782"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO5553567.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            2312..3913
                     /gene="aceB"
                     /locus_tag="ACHJ8C_003783"
     CDS             2312..3913
                     /gene="aceB"
                     /locus_tag="ACHJ8C_003783"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO5553568.1"
                     /translation="MTEQATTIDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISKTASIRDADWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNHQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSRGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            3943..5247
                     /gene="aceA"
                     /locus_tag="ACHJ8C_003784"
     CDS             3943..5247
                     /gene="aceA"
                     /locus_tag="ACHJ8C_003784"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO5553569.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            5372..7096
                     /gene="aceK"
                     /locus_tag="ACHJ8C_003785"
     CDS             5372..7096
                     /gene="aceK"
                     /locus_tag="ACHJ8C_003785"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO5553570.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTGAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDRFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPLPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRFV"
     gene            complement(7113..7937)
                     /gene="iclR"
                     /locus_tag="ACHJ8C_003786"
     CDS             complement(7113..7937)
                     /gene="iclR"
                     /locus_tag="ACHJ8C_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO5553571.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSNHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGIR"
     gene            8137..11820
                     /gene="metH"
                     /locus_tag="ACHJ8C_003787"
     CDS             8137..11820
                     /gene="metH"
                     /locus_tag="ACHJ8C_003787"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO5553572.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPAEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPTELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANTQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKMLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKVSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAE"
     gene            11880..>12180
                     /locus_tag="ACHJ8C_003788"
     CDS             11880..>12180
                     /locus_tag="ACHJ8C_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553573.1"
                     /translation="MIKIDEIDFFAFEGCSESEVTELLGKKPTGYNDWMINIKTGVEW
                     VFYDGRYWLDFRKSKEGIRCISCSILYHDKSKGRIDVLFSEGIADRGLKNTGGVLI"
BASE COUNT         2985 a         3095 c         3260 g         2840 t
ORIGIN      
        1 aaaaaccccg gttaataagg ccggggtttt ttgcatatca attatttgca tgatgaaggg
       61 aatctcatgt cagttctgta tatccaaatt cgtcgtaatc aaattactgt tcgcgatctt
      121 gaaagcaaac gtgaagtgtc aggagatgct gccttcagta accagcgcct gttaatcgcc
      181 aatttctttg ttgcagaaaa agttctgcaa gatcttgttc tgcaactcca cccacgttca
      241 acctggcatt cttttttgcc agcaaaacgt atggatattg ttgtgagcgc gctggaaatg
      301 aatgagggcg gtttgtcaca ggttgaggaa cgcattcttc atgaagtggt cgcaggggca
      361 acgttaatga aatatcgcca gttccacatc catgcgcaat cagtggtact cagtgatagt
      421 gcggtcctgg caatgtttaa gcagaaataa tcatgtcacc attggtgggt acgaaaccta
      481 aagttcagcc caccgggatg agaaaaaaac cgcctacacc cccacatatg ccagattcag
      541 caacggatac ggtttcccca aatcgtccac ctcagagcgt tccgtaacct taaagcccac
      601 cttcttatag aacccaaccg cctgctcatt ttgctcatta acattggttg tcagttccgg
      661 ggccattgag agcgcatgct tcaccagcat ccggcccacg ccgcagccgc gcacatcagg
      721 atcgataaac agcgcatcca tatgctgccc acttaacaac ataaacccaa ccggctgatc
      781 ccgctcatta accgcgaccc acaacggtgc ttccggcaga aaggaatgca ccagctcttc
      841 cagctcggcc cgatactctg ctgataaaaa atcgtgagtg gcatcgacag aacgacacca
      901 aatcgcaacg agttcctccc cctcctcatg ccgtgagcgt cgaatactaa taaccatttt
      961 ctctcctttt cgtcattctt atattctaac gtagcctttt ccttgaaact ttctcacctt
     1021 caacatgcag gctcgacatt ggtaaatttt ctggttatct tcagctatct ggatgtctaa
     1081 acgtataagc gtatgtaatg aggtaatcag gttatgccga ttcgtgtgcc ggacgagcta
     1141 cccgccgtca atttcttgcg tgaagaaaac gtctttgtga tgacaacttc tcgcgcgtct
     1201 ggtcaggaaa ttcgtccgct aaaggtgctt atccttaacc tgatgccgaa gaagatcgaa
     1261 actgaaaatc agtttctgcg cctgctttca aactcacctt tgcaggtcga tattcagctt
     1321 ttgcgcatcg attcccgtga atcgcgcaac acgcccgcag agcatctgaa taacttctac
     1381 tgtaactttg aagatattca ggaacaaaac tttgacggtc tgatagttac cggcgcgccg
     1441 ctgggcctgg tggagtttaa tgatgtcgct tactggccgc agatcaaaca ggtgctggag
     1501 tggtcgaaag atcacgtcac ctcgacgctg tttgtctgct gggctgtaca ggccgcgctc
     1561 aatatcctct atggtattcc taagcaaacc cgcaccgaca aactctctgg cgtttacgag
     1621 catcatattc tccatcctca tgcgcttctg acgcgtggct ttgatgattc attcctggca
     1681 ccgcattcgc gctatgctga ctttccggca gcgttgattc gtgattatac cgatctggaa
     1741 attctggcag agacggaaga aggggatgca tatctgtttg ccagcaaaga taagcgcatt
     1801 gcctttgtga cgggccatcc cgaatatgat gcgcaaacgc tggcgcagga atatttccgc
     1861 gatgtggaag ccgggctaga cccggatgta ccgtataact atttcccgca caatgatccg
     1921 caaaatacac cgcgagcgag ctggcgtagt cacggtaatt tgctgtttac caactggctc
     1981 aactattacg tctaccagat cacgccatat gatctacggc acatgaatcc aacgctggat
     2041 taatcttctg tgatagtcga tcgttaagcg attcagcacc ttacctcagg caccttcggg
     2101 tgcctttttt atttccgaaa tacacctcag caagtgaata aattttattt atattgttat
     2161 caacaagtta atatgtattt ttaattaaaa tggaaattgt ttttgatttt gcattttaaa
     2221 tgagtagtct tagttgtgct gaacgaaaag cgcacaacgg tccttcgttc acagtgggga
     2281 agttttcgga tccatgacga ggagctgcac gatgactgaa caggcaacaa caatcgatga
     2341 actggctttc acaaggccgt atggcgagca ggagaagcaa attcttactg ccgaagcggt
     2401 agaatttctg actgagctgg tgacgcattt tacgccacaa cgcaataaac ttctggcagc
     2461 gcgcattcag cagcagcagg atatcgataa cggaacgttg cctgatttta tttcgaaaac
     2521 agcttccatt cgtgatgctg actggaaaat tcgtggtatt cctgcggact tacaagatcg
     2581 tcgggtagag ataactggcc cggttgagcg caagatggtg atcaacgcac tgaacgccaa
     2641 tgtgaaagtc tttatggccg atttcgaaga ttcactggca ccagactgga acaaagtgat
     2701 cgacgggcaa attaacctgc gcgatgcggt taacggcacc atcagctata ccaatgaagc
     2761 aggcaaaatt tatcagctca agcccaatcc agcggtgttg atttgtcggg tacgcggtct
     2821 gcacttgccg gaaaaacatg tcacctggcg tggtgaggca atccccggta gcctgtttga
     2881 ttttgcgctc tatttcttcc acaaccatca ggcactgttg gcaaagggca gtggtcccta
     2941 tttctatctg ccgaaaaccc agtcctggca ggaagcggcc tggtggagcg aagtcttcag
     3001 ctatgcagaa gatcgcttta atctgccgcg cggcaccatc aaggcgacgt tgctgattga
     3061 aacgctgccc gccgtgttcc agatggatga aatccttcac gcgctgcgtg accatattgt
     3121 tggtctgaac tgcggtcgtt gggattacat cttcagttat atcaaaacgt tgaaaaacta
     3181 tcccgatcgc gtcctgccag acagacaggc agtgacgatg gataaaccct tcctgaatgc
     3241 ttactcacgc ctgttgatta aaacctgcca taaacgtggt gcttttgcga tgggcggtat
     3301 ggcggcattt attccgagca aagatgaaga gcgcaataac caggtgctca acaaagtaaa
     3361 agcggataaa tcgctggaag ctaataacgg tcacgatggc acatggattg cccacccagg
     3421 ccttgcggat acggcaatgg cggtattcaa cgacattctc ggctcccgta aaaatcagct
     3481 tgaagtgatg cgcgaacaag acgcgccgat tactgccgat cagctgctgg caccttgtga
     3541 tggtgaacgc accgaagaag gtatgcgcgc caacattcgc gtggctgtgc agtacatcga
     3601 agcatggatc tccggcaacg gctgcgtgcc gatttatggc ctgatggaag atgcggcgac
     3661 ggctgaaatt tcccgtacct caatctggca gtggatccat catcaaaaaa cgttgagcaa
     3721 tggcaaaccg gtgaccaaag ccttgttccg ccagatgctg ggcgaagaga tgaaagtcat
     3781 tgccagcgaa ctgggcgaag aacgtttctc ccgggggcgt tttgacgatg ccgcacgttt
     3841 gatggaacag atcaccactt ccgatgagtt aattgatttc ctgaccctgc caggctaccg
     3901 cctgttagcg taaaccacca cataactatg gagcatctgc acatgaaaac ccgtacacaa
     3961 caaattgaag aattacagaa agagtggact caaccgcgtt gggaaggcat tactcggcca
     4021 tacagcgcgg aagatgtggt gaaattacgc ggttcagtca atcctgaatg cacgctggcg
     4081 caactgggcg cggcgaaaat gtggcgtctg ctgcacggcg agtcgaaaaa aggctacatc
     4141 aacagcctcg gtgcactgac tggcggtcag gcgctgcaac aggcgaaagc gggtattgaa
     4201 gcggtctatc tgtcgggatg gcaggtagcg gcggacgcta acctggcggc cagcatgtat
     4261 ccggatcagt cgctctatcc ggcaaactcg gtgccagctg tggtggagcg gatcaacaac
     4321 accttccgcc gtgcggatca gatccaatgg tccgcaggca ttgagccggg cgatccgcgc
     4381 tatgtcgatt acttcctgcc gatcgttgcc gatgcggaag ccggttttgg cggtgtcctg
     4441 aatgcctttg aactgatgaa agcgatgatt gaagccggtg cagcggcagt tcacttcgaa
     4501 gatcagctgg cgtcagtgaa gaaatgcggt cacatgggcg gcaaagtttt ggtgccaact
     4561 caggaagcta ttcagaaact ggtcgcggcg cgtctggcag ctgacgtgac gggcgttcca
     4621 acccttctgg ttgcccgtac cgatgctgat gcggcggatc tgattacctc cgattgcgac
     4681 ccgtatgaca gcgaatttat taccggcgag cgtaccagtg aaggcttctt ccgtactcat
     4741 gcaggtattg aacaagcgat cagccgtggc ctggcgtatg cgccatatgc tgacctggta
     4801 tggtgtgaaa cctccacgcc ggatctggaa ctggcacgtc gctttgcaca agccatccac
     4861 gcgaaatatc cgggcaaact gctggcttat aactgctcgc cttcgttcaa ctggcagaaa
     4921 aacctcgacg acaaaactat tgccagcttc cagcagcagc tgtcggatat gggctacaag
     4981 ttccagttca tcaccctggc aggcatccac agcatgtggt tcaacatgtt cgacctggca
     5041 aacgcctatg cccagggcga gggtatgaag cactatgttg agaaagtgca gcagccggaa
     5101 tttgccgccg cgaaagatgg ctataccttc gtatctcacc agcaggaagt gggtacaggt
     5161 tatttcgata aagtgacgac cattattcag ggcggcacgt cttcagtcac cgcgctgacc
     5221 ggctccaccg aagaatcgca gttctaagca accgataaac gttgctgact gtaggccgga
     5281 taaggcgttc acgccgcatc cggcaatcag cgcctgatgt gacgcttacg cgtcttatcc
     5341 ggcaattctc tgctcccgac gagggcaaca aatgccgcgt ggcctggaat tactgattgc
     5401 acaaaccatt ttgcaaggtt tcgacgctca gtatggtcga ttcctcgaag tgacttccgg
     5461 ggcgcagcag cgtttcgagc aagccgactg gcatgctgtc cagcaggcga tgaaaaaccg
     5521 tattcatctt tacgatcatc acgtgggtct ggtggtggag caactgcgct gcattactaa
     5581 cggccaaagc acgggcgcgg catttttact gcgtgttaaa gagcattaca cccggctgtt
     5641 gccagattac ccgcgcttcg agattgcgga gagctttttc aactccgtgt actgtcggtt
     5701 atttgaccac cgctcgctta ctcccgagcg gctttttatc tttagctccc agccagagcg
     5761 ccgctttcgt accattccac gcccactggc gaaagacttt caccccgatc acggctggga
     5821 atctctgctg atgcgcgtta tcagcgatct gccgctgcgt ctgcgttggc agaataaaag
     5881 ccgtgacatt cattacatcg ttcgccatct gacggaaacg ctggggacag acaacctcgc
     5941 cgaaagtcat ttacaggtgg cgaacgaact gttttaccgc aataaggcag cctggctggt
     6001 gggcaaacta ataacgcctt ccggcacgtt gccattttta ttgccgatcc accagacgga
     6061 cgacggcgag ttatttattg atacctgcct gacgacgacc gccgaagcga gcattgtttt
     6121 tggctttgcg cgttcttatt ttatggttta tgcaccgctg cccgcagcgc tggtcgagtg
     6181 gctacgggaa attctgccag gtaaaaccac cgctgaattg tatatggcta tcggctgcca
     6241 gaagcacgcc aaaactgaaa gctaccgcga atatctcgtt tatctacagg gctgtaatga
     6301 gcagttcatt gaagcgccgg gtattcgtgg aatggtgatg ttggtgttta cgttgccggg
     6361 ttttgatcgg gtatttaaag tcatcaaaga caggttcgcg ccgcagaaag agatgtctgc
     6421 cgctcacgtt cgtgcctgct atcaactggt gaaagagcac gatcgcgtgg gccgaatggc
     6481 ggacacccag gagtttgaaa actttgtgct ggagaagcgg catatttccc cggcattaat
     6541 ggaattactg ctccaggaag cagcggaaaa aatcaccgat ctcggcgaac agattgtgat
     6601 tcgccatctt tatattgagc ggcggatggt gccgctcaat atctggctgg aacaagtgga
     6661 aggtcagcag ctgcgcgacg ctattgaaga gtacggcaac gccattcgcc agcttgccgc
     6721 tgccaacatc ttccctgggg atatgttatt taaaaacttt ggcgtcaccc gtcacgggcg
     6781 cgtggtgttt tatgattacg acgaaatttg ctacatgacg gaagtgaatt tccgcgacat
     6841 cccgctgccg cgctacccgg aagacgaact tgccagcgaa ccgtggtaca gcgtctcgcc
     6901 gggcgatgtt ttcccggaag agttccgtca ctggttatgt gccgacccgc gtattggtcc
     6961 gctatttgaa gagatgcacg ccgacttgtt ccgcgctgat tactggcgcg cactacaaaa
     7021 ccgcatacgt gaagggcatg tggaagatgt ttatgcgtat cggcgcaggc agcggtttag
     7081 cgtccgattc gtataaatca gaggcgaaaa agtcagcgta ttccaccgta cgccagcgtc
     7141 acttccttcg ccgctttaat caccatcgcg ccaaactcgg tcacgcggtc atcggtaata
     7201 cgtgaaatcg gtccggaaat tgaaattgcg gcaaacggtt cgcggtgctc atcgaaaata
     7261 cacgctgcaa ggcaacgcag ccccagcgca tgttcctcat cgtcaaatga ataaccccgt
     7321 ttgcgcgttt gggcgagatc ttcttttaaa tgcacaggag acaccagcgt cgcgtgggta
     7381 taggcatgta accctttgcg gtgcagcagc ttcgtcacct gttcttcgct cagttgggcc
     7441 agaaaggctt tacccgcacc ggaagcgtgc atcggcaatt taccgccgat aggcgcggac
     7501 atacgcatca ggtgagtaca ctgtacctgg tcgataataa tcgcttcgtg attgctttga
     7561 tcaagaaccg ccatattgac cgtttcgcca gactcttcca ttagattgcg caggataggg
     7621 tgaacaatcg ctaacaaatt acggctctgg agaaagctgc tgccgaccat aaaggcgtgt
     7681 gcgccgatcg cccagtgacc cagttcgccg acctgacgga caaagccctg ctgttgcatc
     7741 gtggttagca ggcggtgggt cgtggaattg ggtaacccgg cttgctgtgc cagttccgtg
     7801 agtgccacac tgccattgga ttcggcaatc cactccagta atttcaggcc acgcgttaaa
     7861 gactgaacct gtccagtcgc tggtgcggtg gcaacggcgg gttttctgcc gcgttttgcg
     7921 ggaatgggtg cgaccatgac agtctccttt ttctgtatcg tggaaatcat tttcattttt
     7981 attatcagcg aatgcaatag ttgctgaaca gatccgatga gttaatgttg aacaaatctc
     8041 atgttgcgtg gtggtcgctt ttaccacaga tgcgtttatg ccagtatggt ttgttgaatt
     8101 tttattaaat ctgggttaag cgtgtcggga gcaagtgtga gcagcaaagt ggaacaactg
     8161 cgtgcgcagt taaatgaacg tattctggtg ctggacggcg gtatgggcac catgatccag
     8221 agctatcgac tgaacgaagc cgattttcgt ggtgaacgct ttgccgactg gccatgcgat
     8281 ctcaaaggca acaacgacct gctggtgctc agcaaaccgg aagtgatcgc cgctatccac
     8341 aacgcctact ttgaagcggg cgcggatatc atcgaaacca acaccttcaa ctccacgacc
     8401 attgcgatgg cggattacca gatggaatcc ctgtcggcgg aaatcaactt tgcggcggcg
     8461 aaactggcgc gcgcctgtgc tgatgagtgg accgcgcgca cgccggagaa accgcgctac
     8521 gttgccggtg ttctcggccc gaccaaccgc acggcgtcta tatctccgga cgtcaacgat
     8581 ccggcattcc gcaacatcac ttttgaccag ctggtggcgg cttatcgaga atccaccaaa
     8641 gcgctggtgg aaggtggcgc ggacctgatc ctgattgaaa ccgttttcga cacccttaac
     8701 gccaaagcgg cggtctttgc ggtgaaaacg gagtttgaag cgctgggcgt tgaactgccg
     8761 attatgatct ccggcaccat caccgacgcc tccgggcgca cgctctccgg gcagaccact
     8821 gaagcgtttt acaactccct gcgccacgcc gaagcgctga cctttggcct gaactgtgcg
     8881 ctggggccag acgaactgcg ccagtacgta caggaactgt cgcgtattgc ggaatgctac
     8941 gtcaccgccc acccgaacgc cgggctgccc aacgcctttg gtgaatacga tctcgacgcc
     9001 gacacgatgg caaaacagat acgtgaatgg gcagaagcag gctttctcaa tatcgtcggc
     9061 ggctgctgcg gcaccacgcc acaacatatt gcagcgatga gtcgtgcagt agaaggatta
     9121 gcgccgcgca aactaccgga aattcccgta gcctgccgct tgtccggcct ggagccgctg
     9181 aacattggcg aagatagcct gtttgtgaac gtgggtgaac gcaccaacgt caccggttcc
     9241 gctaagttca agcgcctgat caaagaagag aaatacagcg aggcgctgga tgtggcgcgt
     9301 cagcaggtgg aaaacggtgc gcagattatc gatatcaaca tggatgaagg gatgctcgat
     9361 gccgaagcgg cgatggtgcg ttttctcaat ctgattgccg gtgaaccgga tatcgctcgc
     9421 gtgccgataa tgatcgactc ctcaaaatgg gacgttattg aaaaaggtct gaagtgtatc
     9481 cagggcaaag gcattgttaa ctctatctcg atgaaagagg gtgtcgacgc ctttatccat
     9541 cacgcgaaac tgttgcgtcg ctacggtgcg gcagtcgtgg taatggcttt tgacgaacag
     9601 ggccaggccg atacccgcgc acggaaaatc gagatttgcc gtcgggcgta caaaatcctc
     9661 accgaagagg ttggcttccc ggctgaagat atcatcttcg acccgaacat cttcgcggtc
     9721 gcaactggca ttgaagagca caacaattac gcacaggact ttatcggcgc gtgtgaagac
     9781 atcaaacgtg aactgccgca cgcactgatt tccggcggcg tttctaacgt gtcgttctcg
     9841 tttcgtggca acgatccggt gcgcgaagcc attcacgcgg tgttcctcta ctacgctatt
     9901 cgcaatggca tggatatggg gatcgtcaac gccgggcaac tggcgattta cgacgacctg
     9961 cctaccgaac tacgcgacgc ggtggaggat gtgatcctca accgtcgcga cgatggcacc
    10021 gagcgtttac tggagcttgc cgagaaatat cgcggcagca aaaccgacga caccgctaac
    10081 acccagcagg cggagtggcg ctcgtgggaa gtgaataaac gtctggaata ctcgctggtc
    10141 aaaggcatta ccgagtttat cgagcaggat accgaagaag cccgccagca ggctacgcgt
    10201 ccgattgaag tgattgaagg cccgttgatg gacggcatga acgtggtcgg cgacctgttt
    10261 ggcgagggca aaatgttcct gccgcaggtg gtgaaatcgg cgcgcgtaat gaagcaggcg
    10321 gtggcgtatc tcgaaccgtt tattgaagcc agcaaagagc agggtaaaac caacggcaag
    10381 atggtgatcg ccaccgtgaa aggcgatgtc cacgacatcg gtaaaaatat cgttggcgtg
    10441 gtgctgcaat gtaacaacta cgaaattgtc gatctcggcg tgatggtgcc tgcggaaaaa
    10501 attctccgta ccgctaaaga agtgaatgct gatttgattg gcctttcagg gctgatcacg
    10561 ccgtcgctgg acgagatggt taacgtggcg aaagagatgg agcgtcaggg cttcaccatt
    10621 ccgctattga ttggcggcgc gaccacctca aaagcgcaca cggcggtgaa aatcgagcag
    10681 aactacagcg gcccgacggt gtatgtgcag aacgcatcgc gcaccgttgg tgtggtggcg
    10741 gcgctgcttt ccgacactca gcgcgatgat ttcgttgctc gtacccgcaa ggagtacgaa
    10801 accgtgcgta ttcagcacgg acgaaagaaa ccacgcacac caccggtcac gctggaagcg
    10861 gcgcgtgata acgacttcgc ttttgactgg caggcttaca cgccgccggt ggcgcaccgt
    10921 ctcggtgtgc aggaagtcga agccagcatc gaaacgctgc gtaattacat cgactggacg
    10981 ccgttcttta tgacctggtc gctggccggg aagtatccgc gcattctgga agatgaagtg
    11041 gtaggtgttg aggcgcagcg gctgtttaaa gacgccaacg acatgctgga taaattaagc
    11101 gccgaaaaaa tgctgaaccc gcgtggtgtg gtgggcctgt ttccggcaaa ccgtgtgggc
    11161 gatgacattg aaatctaccg tgacgaaacg cgtacccatg tgattaatgt cagccaccat
    11221 ctacgccaac agaccgaaaa aaccggcttc gctaactact gtctcgctga cttcgttgcg
    11281 ccgaaggttt ccggcaaagc ggattacatc ggcgcatttg ccgtgactgg cgggctggaa
    11341 gaggacgcac tggctgatgc ctttgaagca cagcatgatg attacaacaa aatcatggtg
    11401 aaagcgcttg ccgaccgtct ggcggaagcc tttgcggagt atctccatga gcgtgtgcgt
    11461 aaagtctact ggggctatgc gccgaacgag aacctcagca acgaagagct gatccgcgaa
    11521 aactaccagg gcatccgtcc ggcaccgggc taccctgcct gcccggaaca tacggaaaaa
    11581 gccacaatct gggagctgct ggaagtggaa aaacataccg gtatgaaact cacggaatcc
    11641 ttcgccatgt ggccgggtgc atcggtttcg ggttggtact tcagtcatcc agacagcaag
    11701 tattacgcgg tggcgcaaat tcagcgcgat caggttgaag actatgcccg ccgtaaagga
    11761 atgagcgtta ccgaagtcga gcgttggttg gcaccgaatc tggggtatga tgccgagtaa
    11821 gtgataacac cggggtattt gccccggttt ttaatgtcat aaataatgga ttattgaaca
    11881 tgataaaaat tgatgaaata gattttttcg ctttcgaagg atgttcagag tccgaagtta
    11941 ctgaactatt aggaaaaaaa ccaacaggtt ataatgattg gatgattaat ataaaaacag
    12001 gtgtggagtg ggtattttat gatggtcgtt attggttaga ttttcgtaaa tccaaagagg
    12061 gtataaggtg tatctcatgc agtattctgt atcacgataa aagtaaaggg cgtatcgatg
    12121 tcttatttag tgaaggtata gctgacagag gtttaaaaaa tacgggtgga gtgttaatcc
//
LOCUS       DAZFJL010000134        11796 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFJL010000134 DAZFJL010000000
VERSION     DAZFJL010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.136"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            147..1373
                     /gene="mdtG"
                     /locus_tag="ACHJ8C_003789"
     CDS             147..1373
                     /gene="mdtG"
                     /locus_tag="ACHJ8C_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO5553574.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            1456..1830
                     /gene="msyB"
                     /locus_tag="ACHJ8C_003790"
     CDS             1456..1830
                     /gene="msyB"
                     /locus_tag="ACHJ8C_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFO5553575.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAENANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQDENTLHEWDEGE
                     FQLEPPLDTEEGRTAADEWDER"
     gene            complement(1831..2058)
                     /gene="yceK"
                     /locus_tag="ACHJ8C_003791"
     CDS             complement(1831..2058)
                     /gene="yceK"
                     /locus_tag="ACHJ8C_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO5553576.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(2231..4774)
                     /gene="mdoH"
                     /locus_tag="ACHJ8C_003792"
     CDS             complement(2231..4774)
                     /gene="mdoH"
                     /locus_tag="ACHJ8C_003792"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO5553577.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDTASQ"
     gene            complement(4767..6302)
                     /gene="mdoG"
                     /locus_tag="ACHJ8C_003793"
     CDS             complement(4767..6302)
                     /gene="mdoG"
                     /locus_tag="ACHJ8C_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO5553578.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            6696..7853
                     /gene="mdoC"
                     /locus_tag="ACHJ8C_003794"
     CDS             6696..7853
                     /gene="mdoC"
                     /locus_tag="ACHJ8C_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO5553579.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(7861..9342)
                     /gene="clsC"
                     /locus_tag="ACHJ8C_003795"
     CDS             complement(7861..9342)
                     /gene="clsC"
                     /locus_tag="ACHJ8C_003795"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO5553580.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(9284..9817)
                     /gene="ymdB"
                     /locus_tag="ACHJ8C_003796"
     CDS             complement(9284..9817)
                     /gene="ymdB"
                     /locus_tag="ACHJ8C_003796"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO5553581.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(9912..10223)
                     /gene="ymdA"
                     /locus_tag="ACHJ8C_003797"
     CDS             complement(9912..10223)
                     /gene="ymdA"
                     /locus_tag="ACHJ8C_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO5553582.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGRTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(10344..10676)
                     /gene="csgC"
                     /locus_tag="ACHJ8C_003798"
     CDS             complement(10344..10676)
                     /gene="csgC"
                     /locus_tag="ACHJ8C_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO5553583.1"
                     /translation="MNALLLLVALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQLIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(10735..11193)
                     /gene="csgA"
                     /locus_tag="ACHJ8C_003799"
     CDS             complement(10735..11193)
                     /gene="csgA"
                     /locus_tag="ACHJ8C_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO5553584.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(11234..11689)
                     /gene="csgB"
                     /locus_tag="ACHJ8C_003800"
     CDS             complement(11234..11689)
                     /gene="csgB"
                     /locus_tag="ACHJ8C_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO5553585.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         2932 a         3051 c         2793 g         3020 t
ORIGIN      
        1 gggtatccgg cattaccaca gcaaatcccc ctgatttagc gataaaagct ctctggattg
       61 cgccccctgg aagtcgggcg cataattagt gtgcttatct tttcttctta tgttcaccgc
      121 gcctggcgca ccaacagcgg attgctatgt caccctgtga aaatgacacc cctataaact
      181 ggaaacgaaa cctgatcgtc gcctggctag gctgttttct taccggtgcc gccttcagtc
      241 tggtaatgcc cttcttaccc ctctacgttg agcagcttgg cgttaccggt cactccgccc
      301 tgaatatgtg gtccggtatt gtcttcagca ttacattttt attttcggcc atcgcctcac
      361 cgttttgggg tggactcgcc gaccgtaaag gccgaaaact catgctatta cgctctgctc
      421 tcggcatggg catcgtgatg gtgttgatgg gactggcaca aaatatctgg cagtttttga
      481 tcctacgggc gcttcttggt ttacttggcg gatttgtccc caacgctaat gctcttatcg
      541 ccacacaagt accgcgtaat aaaagcggct gggcgctggg tacgctctcc acaggcggcg
      601 ttagcggcgc gttgctcggc ccaatggctg gcggtttgct cgccgatagc tacggcttac
      661 gtccggtatt ctttattacc gccagtgtgc tcatactctg ctttttcgtc accctgttct
      721 gcatcagaga aaaattccag ccggtcagca aaaaagagat gctgcacatg cgggaagtgg
      781 tgacatcact taaaaacccg aaactggtac tcagcctgtt tgtcactacg ttaatcatcc
      841 aggtggcgac gggctcaatt gcccccattc tgacgctgta tgtccgcgaa ctggcgggta
      901 acgtcagtaa cgtcgccttt atcagtggca tgatcgcctc ggtgccaggc gtggcggctc
      961 tgctaagtgc accacgactc ggcaaacttg gcgatcgaat cggacccgaa aagatcctga
     1021 ttacggcgct gatcttttct gtactgctgt tgatcccaat gtcttacgtt cagacgccat
     1081 tacaacttgg gattttacgt tttttgctcg gtgccgccga tggtgcacta ctccccgccg
     1141 tacagacact gttagtttat aactcgagca accagatcgc cgggcgtatc ttcagctata
     1201 accaatcgtt tcgtgatatt ggcaacgtta ccggaccatt gatgggagca gcgatttcag
     1261 cgaactacgg tttcagagcg gtatttctcg tcaccgctgg cgtcgtgtta ttcaacgcag
     1321 tctattcatg gaacagtcta cgtcgtcgtc gaatacccca ggtatcgaac tgatttttcg
     1381 cctttcatac ttgcaaaagc ggagaatcag ctatcctttt ccctgaaacc tcatcaactc
     1441 aaagggagaa tcgtgatgac catgtacgca acgcttgaag aagccattga cgctgcacgc
     1501 gaagaatttc ttgcagacaa ccccggcatc gacgccgaaa acgcgaatgt gcaacagttc
     1561 aatgcccaaa aatacgtttt gcaggacggc gacatcatgt ggcaagttga gttttttgcc
     1621 gacgaagggg aagaaggtga atgtttaccc atgcttagcg gtgaagctgc gcaaagtgtc
     1681 tttgacggcg actatgatga gattgagatc cgccaggagt ggcaagatga gaatacatta
     1741 catgaatggg acgaggggga atttcagctt gagccaccgc tggataccga ggaaggacgc
     1801 actgcagctg atgagtggga tgaacgttaa tcactcatac gggccatgat gtatgtcgat
     1861 cggcagcagt aaagtatcga aaaccaggga gaatggcaga tcaaggatcg tgacgtaacg
     1921 ccaggcggag tcacgcacgt cccattgcac accgggataa tattggttgc catgcccctg
     1981 ccccggtata gtgcggctaa taatgctgcc acagccactc agcaaggtca ccatgatgct
     2041 caccacaatt aaacgcattt gatacctctt ccgtaaaaaa ataccggcat aacgccggta
     2101 tttttattgg ctcgttgatt ttgccagatg tggtgtgaac gccttatccg acctacaaaa
     2161 tcttgcaaaa tcaacacatt gcaggagcga tgtaggcctg ataagcgtag cgcatcagac
     2221 aactacgttt ttattgcgaa gccgtatccg gtttacgcaa tgccagtgga ttgagcttta
     2281 tcccttcgta ataactcacc catgaagaat atctctccgg ggaattccag acgcggaaat
     2341 gcagacgggc catcgtcacc ggatcgctta gcagcaccag gcgacgatcg cgattcagct
     2401 tctctggcgt ctcgttcagc gcctgttcaa cgtggcggtc acgggcgatt tccagcacct
     2461 tgctggcgcg gtgacgcgcg gtcgccattg cggttgccag agcgttaaat gacgggttaa
     2521 acaccgcgtg cataaaacca tcatcaaggg agcgttgacg attcatctca aggaaccgat
     2581 cggtatcaac cagcacctgc ggcggtgaat actcttccgg gatcaggaac agtttccagc
     2641 gtttggttcg cagaccaacg gtggcacggc tggaaatcac cgaaacaaac ggtgacagga
     2701 tcaacgagaa gacaatcggt gccagccaga acagaaaacg cagatccaac cacgccatac
     2761 caactgccca cactaaccct aacagcagct gtgagccgtg gcgtttgaat gcttcgcccc
     2821 aggaagtgga gtcatcatca cgctgcggtg aattccacac cacttcccag ccaaggaacg
     2881 cgctgacgac gaagaccgta tggaacaaca tgcgtacggg agccagcagc acggaaaaca
     2941 gcacttccag cagcaacgat aatgtaacgc gccagaagcc gccgtattct ttcgttcctt
     3001 tgcaccagat aagcaaaatg ctcaatagct tcggcaggaa caacagcacc atggtcgaag
     3061 caaaaagtgc aatcgccagc tcaggacgcc actgcggcca taccgggaac aactgccgtg
     3121 gttgcaggaa gtattgcggt tcggtcaacg catgtactac ctgcaatgca gtagagagcg
     3181 cgaggaacat aaaccacagc ggagcggaga gataagacat cacgcccgtc aggaacaccg
     3241 cacggtgaac cgggtgcata cccttcacca ggaacagacg gaagttcatc aggttaccgt
     3301 ggcaccagcg gcggtcacgt tttagctcat caagcaagtt aggcggcaat tcttcataag
     3361 aacccgggag atcgtaagca atccagaccc cccaacctgc acggcgcatc aacgccgctt
     3421 ccacgaagtc atgtgacagg attgaaccgg caaaagaacc ttcgcccggc agcggagcca
     3481 gtgcacagtg ctcgataaat ggtttcacgc ggataatcgc gttatgcccc cagtagtgcg
     3541 actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag tggcccatac acgcgggtcg
     3601 cgaactgctg acagcgcgca tacagcgtat ccatgccgga cgctttcggc gacgactgaa
     3661 tgatcccagc gttcgggttg gcttccatca ggcgcaccag gccgcacaaa caatcaccgg
     3721 tcattaccga gtcagcatcc agcaccacca tgtagctgta ctggctgccc cagcgacggc
     3781 agaagtcatc gatattaccg cttttacgct tcacgcgacg gcggcggcgg cgatagaaaa
     3841 tctgaccttc gccaccgact tcagcgataa gctccatcca ggctttttgc tctgcgacgc
     3901 agatatccgg gttataactg tcactaagaa tgtagacatc aaaatgtttg gcattcccgg
     3961 tggcttttac tgattcccac gttgcacgca agccagcaaa aacacggttc acgtcttcgt
     4021 tacagatagg catgatcaac gccgtgcgat gctccgggtt taatggttca tcgccaactg
     4081 ttgacgcgga tatactgtat ttatcgcgac caataagcag ttgcaggaag cccatcaacg
     4141 ccgtccagaa tccggcggac acccaacaga acagtaccgc aaagaggatc aggataccgg
     4201 tttgcagcat ataaggcaga agctgcataa aggaaaccca cacatcctga ccaaccatat
     4261 ccataggatt aatcagcgcc cacccctgat aaggaagaat ggtcttcata taccaggtcg
     4321 cgacaacagt ttgcgcgagc gtcaggatca acagaatgta acggcggatg gtaccgacgg
     4381 tacgccactt ttgctcactc tcctgctctt ctttggtcaa acgagccaga tagcgcggcg
     4441 tcacatcgcg tccacgcagg cgatcccaga aacggcctac cgggttggta cgccacgggt
     4501 cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt tagctgatcg cgcccttcgt
     4561 cgtctttaat taactgtcca tcagcaagtg aatctggcca ggcttgttcc agacgcgcct
     4621 ttaccgagcc ttgcggggag tcatcctccc gcgcccaagt gcggtgttcg gcatccagcg
     4681 cctgatgaac ggcgcggata tcagtcttcg gcaatgccgc tttctcgctt gcggcgatgg
     4741 gcattgcgtc aatgtactca gttgtcttat tcattggcag gtaactggta gctccaggtt
     4801 tcactcaacg tctgatcggc attcaccagc gcagcacgca tttcagtggt tttcttggca
     4861 tctttcactt tcacacgcat caccagacgc caacctttag taaccgggtt ataacgcacc
     4921 gtgctttcaa ctatctcacc attatcacca atgctggttt gcgctgtgac cggggtatcc
     4981 tctggcagtt ttttcatctc agcgccggta aaatcgacca caaaggcgat agtaccgtca
     5041 ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg aacgacgcgt ttgttgcacc
     5101 catgcgttat ctggcgcatg cagtttgtct tcatcacggc tgaaggtgat ggtgtattta
     5161 aagttcatct ctttacccgg ctccggcagc tgatccggcg tccagtaagc gacgatgtta
     5221 tcgttggttt catcgttggt tggaatttcc accagctcaa cgctgccttt gccccactcc
     5281 cctttcggag tcacccatgc gcttggacga agatcgtaac gatcatcgag atcttcaaag
     5341 cgggagaaat cacgaccacg ctgcaataga ccgaagcctt gcgggttttc catcgagaag
     5401 ctgctgaccg ctaaatgttt cgggttattc aacggacgcc agatccattc gccattacca
     5461 gcatggatag acagaccgtt agagtcgtgc aactccggac gatagttatt tgcaggcgac
     5521 ggttggttcg gcccaaacag gaacatactg gttaacggtg caacccccag tttgccgact
     5581 ttatcgcgca gatagatttt cgactgcaca tccacaaccg tgtcacgtcc tggcataact
     5641 acgaatttgt aagcacctgt cgcgcgcggc gagtcaagca atgcataaat ggttaaacgt
     5701 ttatcagtcg gttttggacg ctcgatccag aactctttga agcgtggaaa ttcttcaccc
     5761 gatggcaagg cggtatcaat tgccaggccg cgtgcagaaa ggccataaac ctgacctgca
     5821 ccaatcacgc ggaaatagct ggccccgagc atgctgacga tttcatcgtt tttatctttg
     5881 ctgttgatcg ggtaaagcac tttgaaaccg gcaaaaccaa ggtcttttac cgtgtctttg
     5941 tcatgctgaa catcgccgaa agtgaaataa tccgggctgt atttgattcg tttgactgcg
     6001 gtggcagtca cttcatttat tttgaccggg gtatcgaagt acataccctg atggtagaac
     6061 tcgagtttga atggggtctt cagattgttc cagtacgctt tgtcatgatt aaactggatc
     6121 tgctgatagt ccgcgtattt catatcgcgg aaaacggagg gcaagttgct tttgggcgcc
     6181 tcatagcctt tcccggctaa ggattgagct tgctttgcga catcatcaat actgaaagcc
     6241 cagcttgaag atgtatacag ggttaacatt actgcagcac tcaaccaacg cattttcatc
     6301 atttgtagtt tatgtttcat aattagtaag cacttccccc tttgtgtgct tatatcgatc
     6361 cgatctattt taatggataa cctgccattc cgacaacaga atcccggtat cgttcagcac
     6421 actggctcat ggattaagca cctgattgca accgttttca ctttccgtgc ttctcctaaa
     6481 gacagaagca tcgacttcgt gtagggtttg ccagcaaatt tatttatcaa ccgtcttaca
     6541 gacgcttcca ttctggttag tggtagcgat gatgctatgc ataaaaacct tgaagcctgc
     6601 gcctggcgcg gtcaggtcgg tgtcgctatg gcaacaagcc taaaggcata acccgacagg
     6661 gataagacga aaaatccgag attagttaac catatatgaa tccagtaccc gcgcaacgtg
     6721 aatatttcct cgactccatc cgcgcctggc tgatgttgtt agggatccct tttcatattt
     6781 ctttaatcta ttcgagccat acatggcatg tgaatagcgc cgaaccgtca ttgtggctga
     6841 ccctttttaa tgacttcatc cactcgttcc gcatgcaggt gtttttcgtt atatccgggt
     6901 acttttccta catgcttttt ttacgctatc ccttgaaaaa atggtggaaa gtacgtgtcg
     6961 aacgtgtagg gatcccgatg ttaacagcca tccccttact gacattgccg caatttatta
     7021 tgctgcaata cgtcaaaggg aaagcggaaa gttggcctgg gctgtcattg tatgacaaat
     7081 ataatacgtt ggcctgggaa ttaatatcac acctgtggtt tttactggtg ttagtggtca
     7141 tgacgacgct gtgcgtatgg atatttaaac gcatcagaaa taatttagaa aattctgata
     7201 aaacgaataa aaaattctcg atggtaaaat tatcggtgat ttttttgtgc ctcggcatcg
     7261 gttatgcggt aataagaaga acgattttta ttgtgtatcc acccattctg agtaacggca
     7321 tgttcaattt tattgtcatg caaacgttat tttatttgcc gttctttatc ctcggcgcac
     7381 tggctttcat tttccctcat cttaaagcct tgtttaccac gccgtctcgt ggctgtaccc
     7441 ttgcagcagc attggcgttt gtcgcttatt tactcaacca gcgctatggc agtggcgatg
     7501 cctggatgta cgaaaccgag tcggtgatca ccatggtcct cggtctgtgg atggtgaatg
     7561 tggtcttctc ctttggccac cgtttgctta acttccagtc agcgcgggtg acttattttg
     7621 ttaacgcatc gctgtttatc tatctggttc accacccgtt aacgctgttt ttcggcgcat
     7681 acattacacc gcacatcacc tccaactggc ttggttttct ctgtggtctg atattcgttg
     7741 tagggattgc gataattctg tatgaaattc atttacgcat cccgttactg aagtttttgt
     7801 tctctggtaa accggttgtc aagcgtgaga acgataaagc accagcccgt taagccacgc
     7861 ttataataac cattccacgg gcaatatcga cgccagtctg accataaccc gcttccagaa
     7921 actggtggcg ggttcttttt tgagaataat ctctttcttt gcatgacgat caacccagtt
     7981 gatccgtccc cacctgtcca gacggagctg ccaggccgca tcatactggc tctgaataaa
     8041 gcgtttatca attaactgtg ccagcgtttc gctctctatc acgaagccca tttcagtatt
     8101 gagcaatgtt gaacgcggat cgaaattgaa agagccgata aacaccgttt taccatcgat
     8161 gctaaaggtt ttagcatgca ggctggcacc ggaattaccg gttatgccgc gatcatgtaa
     8221 cgtactactt tgttcacgcg tcggcttgag ttcatataat tccacgccat agcggagcaa
     8281 ttttttgcgc cagcgcgcgt atccggcatg gacgacagca acatcgttag cggcaagaga
     8341 attggttagg atcgcaatct ttaccccttt tctcaccatc cgtaagagtt gcgccacacc
     8401 tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg cgttcactgg gtgagcccat
     8461 gatatcgaac aggcgctgcg gtaacagtga atgccgtttt gccttgccct cccctttcgc
     8521 cggatcatca cttaataaac gtgttttcgc ccagataagc ggcaacgttc catcaaccag
     8581 atgatttata aacgggctgg attccatttt gcgtaaataa cgatgcgtca tggcatcgtt
     8641 gtgccaggat gctggtaact cgatgcgatc cgccatttca ccttccggga catccagcac
     8701 ctgctgtaag ggtgaaaccg atttgcaata ccagtagcgg gcgaaatcat cggcaacgtc
     8761 ctctacaact ggtcctattg ccatgacatc taaatccgaa aaaagtggct cttcccctgc
     8821 accaaaatag gcatcaccaa tatttcgccc ccctaccaag gtaaccacgc catcgacagt
     8881 gaaactttta ttgtgcatac ggcgattgag acgggaaaag tcggtgatat aaccaagcgg
     8941 acgtagcaag cgaaacgaga aaggattaaa aagccggact tcaatgcggg gatgactgtc
     9001 aagcaagcgt aaaatgtcgt caagtccggg tgtattgttg tcgtccagca acaaacggac
     9061 gcgaacgcca cgctttgctg cggctaacag ggcggaaaac agtaaccgac ccgacatatc
     9121 gtcctgccag atgtaatact gaacatccag cgtatgctct gccatttcgg ccagacgata
     9181 ccgggcggca aacgcatcca gacttttctc cagagggaaa aggccacact gaccgggatg
     9241 ttgcgaacac agtggcagca ccgcgctcgc cagccggggc aaatcattca tctccttgtt
     9301 gggtaaggag tctttcgtag aggtgggcgt tttcttcatc ataacagaca aagtatacct
     9361 gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt ttttactgcg atttcagccg
     9421 ctgccgcacg agggtaacca taaaccccag tactaattgc aggaaaagcc actgacgtat
     9481 agctgtttgc cgccaccagt cgtaggctat tgagataggc atcctgcaaa agctggtctt
     9541 cgttttgttc accaccacgc cagactggcc cgacggtgtg cactacggct ttagcgggaa
     9601 gatcgcctgc aagcgtaata acggcatgcc ccgtagggca atcgccctgc tgctgcctga
     9661 cttttaaaca ggcatccagc agggccggac ccgctgcgcg atgaatggcc ccatcgacgc
     9721 cgccgcctcc cattaatgac ggattagccg cattcacaat cacatcaacg gccagtttgg
     9781 taatatcacc ctgcacaaca tgaatacgcg ttttcataac gcctccttac accttcttgt
     9841 ttcttaagtg tacagcaggc tttttggtta aatcgcattt ttctgctcag caacgtgagt
     9901 tgttcagaaa cttagttata ttcgatgttc attacagcca gctttttctg tttatcaaga
     9961 tactgcactt ttactgaggc tatttgcgca tttttgacat tgccgctcat taacgctttg
    10021 ctggaatagg ttctgcccgg aacagagcca ttctgtgggc agttcatttc tacgggtgat
    10081 gactgggtgc tgacgtcaca tgccggttcc acaatttggc cataaaaatg gatcacgccc
    10141 ccttgcgcgg tgcttcctgc aatggcaata aaaggaaaaa acaaactgcc gagcataaga
    10201 gagtttaaaa aaggtctgaa catttattct atttcctcaa tgattaatca tccttgaggg
    10261 ttgtgttatc catactttca ggttggctga ttcgcagcag accattctct ccaggttcat
    10321 cttatgctcg atatttcaac aaattaagac ttttctgaag agggcggcca ttgttgtgat
    10381 aaatgaagtg actgtccatc agaaacagta acaactattt tcacccgatc gtccggggaa
    10441 atatttaaac tcaacttcgt caaagcaatg agttgattag caggcaatga aagggtcttt
    10501 tcttgcttcg tctgactttg ccctgaactg ccttcgcgca gggacaatat ttgtactctg
    10561 cacagacaag attgagtaag agtgacttca ggaataatgg tgtacatatc cccttgctgg
    10621 gtcgtattaa aggttatctg actggaaagt gccacaagga gtaataacgc attcatattc
    10681 ttctcccgaa aaaaaacagg gcttgcgccc tgtttctttc atactgatga tgtattagta
    10741 ctgatgagcg gtcgcgttgt taccaaagcc aacctgagtg acgttaacgc tggagttaga
    10801 tgcagtctgg tcaacagcag caccgttacc gccaccgaac tgtttaacag tcatagtaga
    10861 atctttacca ttccactgat caagagtagc gctgttgccg aaaccacgct gagtcagatc
    10921 gattgagctg tcatcagaac cttggccaac atctgcgccg ttaccgccgc cgtgctgggt
    10981 aatggtcaga tcagagttac gggcgtcagc ttgcagagca agagcagagt taccgccacc
    11041 gtactggtaa atattcagct ctgaattcgg gccgctgtta ttaccgccac caccgtggtt
    11101 gccaccgccg ccgccgtact gaggaacaac acctgccaga gcgctaccgg agaatacgat
    11161 tgctgcaatt gctgctactt ttaaaagttt catgtaaaac ccccatcgga ttgatttaaa
    11221 agtcgaatgg aaattaacgt tgtgtcacgc gaatagccat ttgcgactgt ctctgcacta
    11281 caattgccgt tttttgagta ccatactgtg taatatttgc tttattacca gaacctttct
    11341 ggataatcat cgcagtatta ccataagcac cttgcgaaat actggcatcg ttggcactgc
    11401 ccgcctgatc aatatatgca aggttataat ctcctgtctg atcaatcttt gcccggttgc
    11461 tactaccttc ttgcgcaaca accgccaaaa gttttgagcc tccctgccgt aactgagcac
    11521 tattattagt cccagcttga ccaattatgg ctgcctgatt aaatgaagac ttactcaatt
    11581 catttaccgc aaagttatat tctgaattag ctaaatcata acctgctgcg gctgcaatcc
    11641 caggcgcacc cagtattgtt aacatcataa ataacaattt gtttttcatg ttgtcaccct
    11701 ggacctggtc gtacatttaa gaaattaaat catttcaact tggttgttaa cgcaacctgt
    11761 attttgttaa cgctgcgtta cgatggaaag tatgtc
//
LOCUS       DAZFJL010000135        11458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFJL010000135 DAZFJL010000000
VERSION     DAZFJL010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.137"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..407
                     /locus_tag="ACHJ8C_003801"
     CDS             <1..407
                     /locus_tag="ACHJ8C_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFO5553586.1"
                     /translation="IYDWAGEYRNHPVVVGDKERPTMEHNEVKKSLKACLRGCTKKEL
                     SKINSKAELVHKLATLHAEIAWIHPFQDGNGRSIRLFLQIVATTMGYEFDMEKLDGDV
                     RNKRAYHYAVRRAIHDSNRNLIALISRAIKEL"
     gene            complement(404..601)
                     /locus_tag="ACHJ8C_003802"
     CDS             complement(404..601)
                     /locus_tag="ACHJ8C_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553587.1"
                     /translation="MSKKVITIQVRGGHAGAKPVRRSKLEQSVNRSLRASFSLEGNHI
                     TNTSWSKMSQAARFLTRVAVA"
     gene            complement(778..3060)
                     /locus_tag="ACHJ8C_003803"
     CDS             complement(778..3060)
                     /locus_tag="ACHJ8C_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO5553588.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELCDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWTHQLKGQRMRWHEALLIAVGEVNKGRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVRKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVSVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWSDPEVVRALRGALKHGRRTPSRQ
                     QRNGSPLKPHEIAPSARLTRSERMQITRIRVDLAQNGIRPQRWELEALARGATVNYDG
                     KKFTYPVVDEWRWYIVDGRR"
     gene            complement(3050..3325)
                     /locus_tag="ACHJ8C_003804"
     CDS             complement(3050..3325)
                     /locus_tag="ACHJ8C_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFO5553589.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLSVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNVQVEQLSKRCSEAFGSYGR"
     gene            complement(3322..3546)
                     /locus_tag="ACHJ8C_003805"
     CDS             complement(3322..3546)
                     /locus_tag="ACHJ8C_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HFO5553590.1"
                     /translation="MPDNVDFIQEQQTELLERQINAARVKHCGASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRTA"
     gene            complement(3549..3848)
                     /locus_tag="ACHJ8C_003806"
     CDS             complement(3549..3848)
                     /locus_tag="ACHJ8C_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFO5553591.1"
                     /translation="MSCSHSVVLLNNALKIAVMENGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVINLLVKRAVFHKQISSVDELTKLTTELASYCADVSRKLNDKRC"
     gene            complement(3848..4072)
                     /locus_tag="ACHJ8C_003807"
     CDS             complement(3848..4072)
                     /locus_tag="ACHJ8C_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFO5553592.1"
                     /translation="MHTVSKNQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(4136..4636)
                     /locus_tag="ACHJ8C_003808"
     CDS             complement(4136..4636)
                     /locus_tag="ACHJ8C_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFO5553593.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(4806..5078)
                     /locus_tag="ACHJ8C_003809"
     CDS             complement(4806..5078)
                     /locus_tag="ACHJ8C_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFO5553594.1"
                     /translation="MDAENYVIQYPLDAVHVDKFADLLGKPKTAVSEMVKANKLPIIE
                     LRDPCKPKARAGEKWVFIPEFNRAVREAFYNRPVEQRDAWLLWMGL"
     gene            5215..5508
                     /locus_tag="ACHJ8C_003810"
     CDS             5215..5508
                     /locus_tag="ACHJ8C_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5553595.1"
                     /translation="MKLSEKIKALREAEGLSQSKFCEIIELPLSTLKKYEGGNFEPGG
                     TALLKITMHPTFQKYALWLMTDKTAPDAGQIAPALAHIGPESTESNHSAKRIG"
     gene            5578..6558
                     /locus_tag="ACHJ8C_003811"
     CDS             5578..6558
                     /locus_tag="ACHJ8C_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5553596.1"
                     /translation="MAIKKLDDGRYEVDIRPRGRDGKRIRRKFERKAEALAFERYTIA
                     NASQKEWGGQRADRRTLSELLDIWWKYHGQNHEHGTKEFNHLLKTISGIGDIPVSRMS
                     KRALMDYRSMRLRDGISAATINRDMYRLSGMFTKLIQLDEFSGQHPIHGLPPLAEANP
                     EMTFLEKAEIEKLLNVLAGDDLLVALLCLSTGGRWTEVATLKPAQITNCRVTFLKTKN
                     GKKRTVPISEELEKKVKEEASAKLFKVDYEKFCGILRRVKPDIPPNQATHILRHTFAS
                     HFMMNGGNIIALQQILGHASIQQTMAYAHLAPDYLQNAVALNPLKGGVTL"
     gene            complement(6745..7245)
                     /gene="cpxP"
                     /locus_tag="ACHJ8C_003812"
     CDS             complement(6745..7245)
                     /gene="cpxP"
                     /locus_tag="ACHJ8C_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO5553597.1"
                     /translation="MRIVTAAVMASILAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            7395..8093
                     /gene="cpxR"
                     /locus_tag="ACHJ8C_003813"
     CDS             7395..8093
                     /gene="cpxR"
                     /locus_tag="ACHJ8C_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO5553598.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALSLNPGRQEATFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            8090..9463
                     /gene="cpxA"
                     /locus_tag="ACHJ8C_003814"
     CDS             8090..9463
                     /gene="cpxA"
                     /locus_tag="ACHJ8C_003814"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO5553599.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGEGKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(9568..10242)
                     /gene="yiiM"
                     /locus_tag="ACHJ8C_003815"
     CDS             complement(9568..10242)
                     /gene="yiiM"
                     /locus_tag="ACHJ8C_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO5553600.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTDLGLEGDEQ
                     AEKKIHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKLEDFS
                     RRLWGK"
     gene            complement(10391..11375)
                     /gene="kdgT"
                     /locus_tag="ACHJ8C_003816"
                     /pseudo
     CDS             complement(10391..11375)
                     /gene="kdgT"
                     /locus_tag="ACHJ8C_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
BASE COUNT         2978 a         2937 c         2723 g         2820 t
ORIGIN      
        1 atatttatga ttgggcaggt gaatacagaa accatcccgt tgttgttgga gataaagagc
       61 gaccaacaat ggaacataat gaagtcaaaa aatcattaaa agcctgtctt agaggttgca
      121 caaaaaaaga acttagcaaa ataaattcta aagcagagtt agttcataaa ctagctactc
      181 tccatgcaga aatcgcatgg atccacccct ttcaagatgg taatggtcgt tcaatacgat
      241 tatttcttca gattgttgca actactatgg gttatgaatt tgatatggaa aagctagatg
      301 gagatgtgcg aaataaacgt gcgtaccatt atgctgtaag acgcgcaata cacgacagta
      361 acagaaatct gattgccctt atttcaaggg caatcaaaga gctttaagca acagctactc
      421 tggtcagaaa acgtgcagcc tgcgacattt tagaccatga agtgttggta atgtgattac
      481 cttccagaga aaacgaagca cgcagggaac gattaacact ttgttcaagt tttgaacgac
      541 gaacaggctt agcacctgcg tgcccacctc ttacttgtat cgtgataacc tttttgctca
      601 tattagtctc tctagtggtt aagaacatca taaatcaaca gctaatgata accattatca
      661 tctggaatca tcatagccgg ctggcgattc tacgcctgat tgttcatatc tacaacaact
      721 actttataca tacaaatcta cttacctacc aataacagtg ttcacatctt caacttttca
      781 ccgcctacca tcaactatat accaccgcca ctcatcaaca accggatacg tgaatttttt
      841 cccatcataa tttacggtcg cgccacgcgc cagcgcctca agctcccatc gctgcggcct
      901 gataccgttc tgagcaaggt caacgcggat acgggtaatt tgcattcttt ccgaccgggt
      961 cagtctggcc gatggtgcaa tttcatgtgg ttttaacggg cttccgtttc tttgctgacg
     1021 acttggcgtt ctccgaccgt gttttaatgc acctctgagc gccctcacga cctccgggtc
     1081 actccattcg ataacaccgt catcaaccag attaagcact gctgcggcgt gttcagaagg
     1141 tgtgggagcc ggtaacgaag tatcaccacc ggtgagtttt ccacagttat tgacaggact
     1201 ccgaggcgcg gcgatgccgc tttttaaagt caaaggctca acgaccgaaa ctttcggcac
     1261 aatgcgccag tccgtcgttc tggtgatatg aatatgacgc gcgccgagat gcggcgcgta
     1321 aatgccgacc accctctcga cctcttcctc gtactcgtta acgtcatccg acgggctacg
     1381 ggcgaccctg acagtctgac aatcgcgcgg gacatttgcc ccaccctgcg cgctgatata
     1441 caacgcaaaa tcaccactgt ctgcggcggc gcgtgcagcc tcgacgcgct cgtcaaactc
     1501 atcagcaatg ctgacgccgc gcggcaattt gcgtagttca cggtaagccc ccattgtcgg
     1561 cagaccaacc gttttaaatt gcgggatgcg ccacgttgac gcccatgcgg taacagccgc
     1621 agcagtgtct ttcagcggcc tgccggtatc gttatcgagc tgaccatcca gtgcatagcc
     1681 gtcgatattt tttgagatgt atttcgcgat ataccccgca gcaccgcccc ggttaaggtg
     1741 ttttgcctga aaacggtttc gcgcggctcc tctttcgtcg ccatcctctt tgagcgcata
     1801 gcgacgcatg atttcgataa tctggttacg ctggcgcgga ttacaaaaaa gcatcatatg
     1861 ccagtgcggc gttccgtcgt ggtgtggctc gacgacacgc aaaccgtaga cctgtaaatc
     1921 attatccttg aatgccgtgc gcatcaggct ccagatacgg cagagataac gctgcgcatc
     1981 ctttggatta aatgcctcat cgttccagcc gtgatttagc tggacggttt tactttcgcc
     2041 ttttctgacc tgacgtgtcg ggtgatactt tgacggcgcg gtcagcgtga taaacatccc
     2101 cacatcaccc tctgctgcgg cgtaacgctc aataccggca atggtgttca tcagctccat
     2161 ccggcgaatt tcaggattag aaatactgcc catcacctta ctgataaggt cgatgcgctc
     2221 gccggtttcc ctgttttcga gatcacacga tttaagaaat tccagatttg cctggcggcg
     2281 tgcacgcaca tcacgaatgg catgtttact ggcataagga gaacggcctt tattgacctc
     2341 cccgacagca atcagtaacg cctcatgcca gcgcatacgc tggcctttaa gctgatgggt
     2401 ccaccactca tcgttaaaca gacgggcaat ggcagaatat gcctgcctcg tggtcatctg
     2461 ccctttacgg tattttttcc agtagagagg ggaaatattg aaagcacgtg cagcaccagc
     2521 aacatgacca tagaggtgag cctgcgcctc atccgtaaac agcgattctt tttcgccatg
     2581 cgcatccacc caggcatcgc agagttcctc atacatcatg aaaagctgcg atgagatacg
     2641 ggcggcaaac tttttcagct ccttgtcatt catccccggc agacgcgcat aatggtcacg
     2701 ctctgccaga aacagcaacg acgcgtcggt gttcatttca tggcgctgat tcacacgctc
     2761 aatgcgcggc cataaacgac gctgaaaagt ggatgtgaga aaataaaacc cgtgcaccgg
     2821 gcttttatta cgccggatgt agtcatagcg tgaagtaaac agcgagcgca aaaagtaagg
     2881 caggcggtta atcgtggata aaacaccttg cacctgacgc atctcgtcac gtgtaagggg
     2941 tctttcgcgc ccgacagcct cgcgtggcgc gttccatgca taagcaccgg taaacgcctt
     3001 accggtgcct gcggcaaatg ctgacggagg gacaaaacgc ccggaggctt taacggccat
     3061 atgagccaaa agcctctgaa caacgcttgc tgagttgctc aacctgcacg tttaaatcag
     3121 caaaagactt tgcgcttccg gtcagaatat cgtgatgcat caggccggaa acgagctggc
     3181 ttaatttcgg gtaataacca accaccgaca gccattcctg accggcgttt ttaccgcttt
     3241 ccgctctctt tttctcgtgg agaataaact gaaagctgtc actggtaacg acataacgtt
     3301 cgccaatttc aatacgaata ctcatgccgt tctccggtaa tgtttgtttt ttgcttcaaa
     3361 gactgactga caggaaacac aacgcgtggc tgacggataa gccgcacgac gggcagcagg
     3421 tattggcgcg tcacactctt cgcaaaccag cgcagaagca ccgcaatgtt ttacccttgc
     3481 cgcgttaatc tggcgctcca gtaattcagt ctgttgttcc tgaataaaat ctacgttgtc
     3541 cggcattatc agcacctttt atcgttaagt ttcctggata catcagcgca ataactggca
     3601 agttctgtcg ttaattttgt cagttcatcc acggaggaaa tttgcttgtg gaatacagcg
     3661 cgtttaacaa gtaaattgat cacatcagac aggaggttta attcattctg ataaatcgcg
     3721 ataacagatt cagttatgtc gcgtttttct ttatcaagac aaagttgaat aagagacaaa
     3781 tcaccatttt ccataacggc gatttttaag gcgttattca gtaatacaac tgaatgagaa
     3841 caggacatca aagcacctcc ccgcgagaca atccgatatt gtgaaatttt tccgactcct
     3901 gactgagcag ctcgactatc tccacgcggg ataactccgc ctttgttatg tggcgaatca
     3961 tggcgtcaag atgagaagaa aagcgcgtcg cagcgtcggc ctgtgcttcg gttctggcct
     4021 gttgcagcag taatgcgtat ttaccgcact gatttttaga aactgtatgc atgactttct
     4081 ccaggcaaaa agaagccccg cacgattaag tgcgttaaaa actctggtta attacttaat
     4141 gcagatattg ctctggtttt accgacgtca gaattgtcgg tgcatactca aacaggctga
     4201 ataattcacg taatgcacgg aataaagcat cacgccagta acatgattct tcattaattc
     4261 gccagtatgg ctggttgaat tctttttcag tcaatccggc atgcataaat aaagtacgac
     4321 gctgactgac tgttaaaaaa ctaatatatg catactcact tgcgccaacc tgacggcgtt
     4381 ttgagaatgc cccacgcaat tcatcaattg cacaaaccag ccgttcacgt tcgacgtcgt
     4441 tcatttcttc aaaacgcatc gttgcgtgac gctgttttaa ctgcgcatgg aagcaaaccg
     4501 ttagccgttc gcgttccatc atctgattat aataatcaca tgtatcctgc cagcgaggga
     4561 cagcaagatg cttgccaatt atccggcgca tagctgctgg ctgtttttca acgagattga
     4621 gcgtcatcac tgtcatttcc agaccctccg gcttttcaga aaggtcagag ccttttttaa
     4681 cggactctgt tttttggtgc ggataatgat tcccttgcgt cccttcccgt gggtgattgt
     4741 gaagtcaatc gccctggggc tttcgttacg cagtaactga gcaatacaac gcggctcatt
     4801 cataatcaca accccatcca caaaagccat gcatcacgct gttcaaccgg tcggttataa
     4861 aacgcctcac gtacagcgcg attaaactca ggaatgaaaa cccatttttc accggcacga
     4921 gccttcggtt tgcaaggatc acgcaattca ataattggta atttatttgc cttcaccatt
     4981 tcactgacag ctgtctttgg cttccctaat aaatcagcaa atttatccac atgaaccgca
     5041 tcaagcggat actgaatcac ataattttca gcgtccatat atggtaccct cataggatcc
     5101 agccctttct aaaccactca aaaccgttta gacgctggtt tattctcaaa tcaatggaac
     5161 ctatataggt tccagttttg agggaattta gtccctatat aggcaccatg tcaaatgaaa
     5221 ttaagcgaaa agattaaggc cttgcgtgag gctgaagggc taagccaatc aaaattctgt
     5281 gaaatcatag agttaccgct aagcacactt aaaaaatatg aaggaggaaa ctttgaaccc
     5341 ggtggcacag ctttgctaaa aatcactatg catcccacat tccaaaaata tgctctatgg
     5401 cttatgacag ataaaaccgc gccggacgca ggacaaatcg caccggctct cgcgcacatt
     5461 gggccagagt caacagagtc caaccactcc gcgaaaagga ttggctaact ctatataaag
     5521 attacatttt caccatttgc taccaagatg gtgaatacag cgccggaggg ctttcttatg
     5581 gcaattaaga agctcgatga tggtcgctat gaagtggaca ttagacctcg cggtcgcgac
     5641 ggaaaacgca tccgcaggaa attcgaaaga aaagctgaag cactagcatt tgagcgatac
     5701 acaatcgcca atgccagtca gaaagaatgg ggaggccagc gagcagaccg ccggactttg
     5761 agtgagttgc tggacatctg gtggaaatat cacgggcaaa accacgagca tggaacaaaa
     5821 gagtttaatc atctactcaa aaccatcagc ggcataggtg atataccagt gagcaggatg
     5881 agcaaaaggg ctttgatgga ttatcgttcc atgcgactac gtgatggtat cagtgccgca
     5941 acgataaacc gtgacatgta ccgattatcc ggcatgttca caaaattaat tcaattggat
     6001 gaattttccg ggcaacaccc aattcacgga ctgccgccac tggcggaggc caaccctgaa
     6061 atgacgttcc tggaaaaagc agaaatcgaa aaactgttaa atgttttggc tggtgatgac
     6121 ttacttgtcg cgcttttatg tctgagcact ggaggaagat ggacggaagt tgccacgcta
     6181 aaaccagcac agattacaaa ttgcagggtt accttcctga aaaccaaaaa cggtaaaaag
     6241 cgaaccgtgc cgatttctga ggaactggag aaaaaagtta aagaggaggc cagcgccaaa
     6301 ttattcaaag ttgattatga gaaattttgc gggattttac gcagagtgaa acctgatata
     6361 ccacccaatc aggcaaccca catcctgcgg catacattcg caagccattt catgatgaat
     6421 gggggcaata taatcgcact gcaacagatt ctgggacatg cgagcattca gcagacgatg
     6481 gcctatgcgc accttgcgcc tgactatctg caaaatgccg tcgctctgaa tccactaaaa
     6541 ggcggagtga cgttataaat ttccccctct gagtgtccac atagtgtcca cactcaagaa
     6601 attttgaaac cgctccaaac ccttacagct ttcgataagt acctgttttt acttttgatt
     6661 gcatgtaagt aattgataaa aaaaaccccc acatcatgtg ggggaagaca gggatggtgt
     6721 ctatggcaag gaaaacaggg tttactactg ggaacgtgag ttgctactac tcaatagctt
     6781 caacgatgaa cttttttgcc attgcgtcac gtcgcgcaac tgctccattc gttgttgatg
     6841 tttctcgttt aaaaccgctt gctgctccgg cgttaacagg cgatacattt ggttgcggac
     6901 ttttgccatc tcaacctgac gagcaatttg ctcattcgcc attttttctg cctgtgcgcg
     6961 cacagcgttt tcatcaaaat tttctgcggt gacaaggcga tgcattgtct ccagttcgct
     7021 aacattaaca ggaggctgtt cgtgccgggc ctgttgcata agatctcgca tctgctgacg
     7081 ctgatgttcg gttaaactta tgccgtcgaa catatggctc tgcgtactgc gctgcgtaag
     7141 ttcttcaccc ggatgccagt tatcgcctga accgacttca gcagcgtggc ttaatgaact
     7201 gactgccagt attgaggcca tgacggcagc ggtaactatg cgcatcattt gctcccaaaa
     7261 tctttctgtc gcgattcaac gatagagagt ttacgattca ggctgcaaac atgcgtcagg
     7321 gggtgtaaaa caacgtaaag tcatggatta gcgacgtctg atgacgtaat ttctgcctcg
     7381 gaggtattta aacaatgaat aaaatcctgt tagttgatga tgaccgagag ctgacttccc
     7441 tattaaagga gctgctcgag atggaaggct tcaacgtgat tgttgcccac gatggggaac
     7501 aggcgcttga tcttctggac gacagcattg atttactttt gcttgatgta atgatgccga
     7561 agaaaaatgg tatcgacaca ttaaaagcac ttcgccagac acaccagacg cctgtcatta
     7621 tgttgacggc gcgcggcagt gaacttgatc gcgttctcgg gcttgagctg ggcgcagatg
     7681 actatctccc aaaaccgttt aatgatcgtg agctggtggc acgtattcgc gcgatcctgc
     7741 gccgttcgca ctggagcgaa caacagcaaa acaacgacaa cggttcaccg acactggaag
     7801 ttgatgcctt aagtctcaat cctggtcgcc aggaagccac cttcgacggg caaacgctgg
     7861 agttaaccgg tacggagttt accctgctct atttgctggc acagcatctg ggtcaggtgg
     7921 tttcccgtga gcatttaagc caggaagtgc tgggcaagcg cctgacgcct ttcgatcgcg
     7981 ctatcgatat gcacatttcc aacctgcgtc gtaaactgcc ggatcgtaaa gatggtcacc
     8041 cgtggtttaa aaccctgcgt ggtcgcggct atctgatggt ttctgcttca tgataggcag
     8101 cttaaccgcg cgcatcttcg ccatcttctg gctgacgctg gcgctggtgt tgatgttggt
     8161 tttgatgtta cccaagctcg attcacgcca gatgaccgag cttctggata gcgaacagcg
     8221 tcagggtctg atgattgagc agcatgtcga agcggagctg gcgaacgatc cgcccaacga
     8281 tttaatgtgg tggcggcgtc tgttccgggc gattgataag tgggcaccgc caggacagcg
     8341 tttgttattg gtgaccaccg aaggccgcgt gatcggcgct gaacgcagcg aaatgcagat
     8401 cattcgtaac tttattggtc aggccgataa cgccgatcat ccgcagaaga aaaagtatgg
     8461 ccgcgtggaa ctggtcggtc cgttctccgt gcgtgatggc gaagataatt accaacttta
     8521 tctgattcgt ccggccagca gttctcaatc cgatttcatc aacttactgt ttgaccgccc
     8581 gctattactg ctgattgtca ccatgctggt cagtacgccg ctgctgttgt ggttggcctg
     8641 gagtctggca aaaccggcgc gtaagctgaa aaacgctgcc gatgaagttg cccagggaaa
     8701 cttacgccag cacccggaac tggaagcggg gccacaggaa ttccttgccg ccggtgccag
     8761 ttttaaccag atggtcaccg cgctggagcg catgatgacc tctcagcagc gtctactttc
     8821 tgatatctct cacgaactgc gcaccccgct gacgcgtctg caactgggta cggcgttact
     8881 gcgccgtcgt agcggtgaag gcaaggaact ggagcgtatt gaaaccgaag cgcaacgtct
     8941 ggacagcatg atcaacgatc tgttggtgat gtcacgtaat cagcagaaaa atgcgctggt
     9001 tagcgagacc atcaaagcca accagttgtg gagtgaagtg ctggataacg cggcgttcga
     9061 agccgagcaa atgggcaagt cgttgacggt taacttccca cctgggccgt ggccgctgta
     9121 cggcaacccg aacgccctgg aaagtgcgct ggaaaacatt gttcgtaatg ctctgcgtta
     9181 ttcccatacg aagattgaag tgggctttgc ggtagataaa gacggtatca ccattacggt
     9241 ggacgacgat ggtcctggcg ttagcccgga agatcgcgaa cagattttcc gtccattcta
     9301 tcggaccgat gaagcacgcg atcgtgaatc tggcggtaca ggtttggggc tggcgattgt
     9361 agaaaccgcc attcagcagc atcgtggctg ggtgaaggca gaagacagcc cgctgggcgg
     9421 tttacggctg gtgatttggt tgccgctgta taagcggagt taaactccgc atttgtaggc
     9481 aggataaggc gtttacgccg catccggcat ttgagcagga tgcctgatgc gacgctgata
     9541 gcgtcttatc aggcctacac tccggggtta ttttccccac aaacggcggg aaaaatcttc
     9601 gagcttgccg ctcagtcggc gcttttgcat cgttcgcgtc cagcttttcg ataaccctgc
     9661 ggcggagagt aaacggtgat actgatcgtc atcaaacggc atatgccatg cgatggcggc
     9721 agcctcctgc acggtcacat cgctgacacg ggaaaccaac tccagcggcg catcagcaga
     9781 taccaaccct ggtgcaatta cgctatacaa ccaccccact ttacccgtgt tttgcatcaa
     9841 ctgcgctata tcgctgatat caaaatggta attgagttta tagcaaggtg agcgtggctg
     9901 gctgacctga attaatgcct ctccccagcg gaaaatatca ccaatataaa cattactttc
     9961 cgtcagaccg tcggttgaga gattttcgcc aaacgcaggc gcaacaaaca gctccgcctg
    10021 ttccggaaat tcccgcgccc agtagagata atgctcacga ggataatgac acagcgctct
    10081 gtctggcccg ccgtggatct ttttctccgc ctgttcgtca ccttccagcc ccaggtccgt
    10141 cagcatcaac tcaccatcaa cctggatttt ggcaattgcg ctgggtttgc cttcgggata
    10201 agcctgaatc ttgcctgtgt atacatcaac cggatatcgc atctgttccc tctccacgag
    10261 aaatttcccc attagaccat atttatttta tgtcaggcgt gctgttttcg cggtaagtat
    10321 ccaggcagaa acgcaattga aaaccggacg aacgttagtt catccggctg tggggattaa
    10381 ggaagttggt ttatttcacc gtacctaaaa tttcgatttt cgccgctctg gctttgactt
    10441 tacgtgacca gatagaggta agaattggca ccagaatcga agtcacaatg accgccgtcg
    10501 cgaccagcga agttgctgcc ggagccatcg gtttaaacgc aggcaccatt tcagcaatca
    10561 gcacaggtgt cgctaccgcg gcccctgcgg aactggaagc ggcaattccg gcagtgccat
    10621 cgccaccgcc aatcaattta tcggcgataa tcaacggaat accggtcacg ataattactg
    10681 ccacacccaa caggctcccc agcaaacctg tctgggcaat tacagtcaaa tcaatggtat
    10741 tgcccagtgc gaaggcaaag aatggaataa gcgtctgcac cgctttgctg aaaaattctc
    10801 gtaactcagg gtcaaggttc cccagggcaa agcccaccag gaacggcaat actgcgccga
    10861 cgaaaacatg cggttcaaac gaggcaatcc cggcagtgcc cagaataatc atcgtcatga
    10921 gcggcccgga ctccaacgac atcaacacaa atgccccagc ttcttctttt gtgccgtact
    10981 gctgcatgat ggaagcgtaa agtccgccgt tggtcatatc catcgccgcc accagcgcca
    11041 acgttgaaag tccggcaaaa aatccaactt caacaccatg ttccggaata atgcgcgagg
    11101 caattgccgc aaccacccac gcgacggcaa ttttagttac caccagcgta ccggatttac
    11161 gcagtaccgt tcccgtcgcg cttaatttta ttgacgcccc catgcaaaaa aaccacaccg
    11221 ccagaatggg caccgtaccg gtaatcatcc cgttggtgaa tgatccaaaa tatttccccg
    11281 cccccggcga gaaggtgtgg cacagtgcgc caaggaatag cgggacgagc atcatccccc
    11341 cccgggattt tctcaatcga gcgttttatc tgcatttcca tcacctgtcg gcatattttt
    11401 aagggctgat gcgtcattcc tcaacggagc atcagtgttg atggaaacga agataaac
//
LOCUS       DAZFJL010000136        11446 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFJL010000136 DAZFJL010000000
VERSION     DAZFJL010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.138"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            133..267
                     /locus_tag="ACHJ8C_003817"
     CDS             133..267
                     /locus_tag="ACHJ8C_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO5553601.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTEKGKGKWKIITA
                     "
     gene            246..350
                     /gene="speFL"
                     /locus_tag="ACHJ8C_003818"
     CDS             246..350
                     /gene="speFL"
                     /locus_tag="ACHJ8C_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFO5553602.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            785..2983
                     /gene="speF"
                     /locus_tag="ACHJ8C_003819"
     CDS             785..2983
                     /gene="speF"
                     /locus_tag="ACHJ8C_003819"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO5553603.1"
                     /translation="MSELKIAVSRSCPDCFSTHRECVNIDKSNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLELNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFKFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     EADAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2980..4299
                     /gene="potE"
                     /locus_tag="ACHJ8C_003820"
     CDS             2980..4299
                     /gene="potE"
                     /locus_tag="ACHJ8C_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO5553604.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESECANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSADEGYFPKIFSRVSKVDAPVQGMLTIVIIQS
                     GLSLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4356..5996)
                     /gene="pgm"
                     /locus_tag="ACHJ8C_003821"
     CDS             complement(4356..5996)
                     /gene="pgm"
                     /locus_tag="ACHJ8C_003821"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO5553605.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(6022..6567)
                     /gene="seqA"
                     /locus_tag="ACHJ8C_003822"
     CDS             complement(6022..6567)
                     /gene="seqA"
                     /locus_tag="ACHJ8C_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO5553606.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6752..7516
                     /gene="ybfF"
                     /locus_tag="ACHJ8C_003823"
     CDS             6752..7516
                     /gene="ybfF"
                     /locus_tag="ACHJ8C_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5553607.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGILARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7656..7949
                     /gene="ybfE"
                     /locus_tag="ACHJ8C_003824"
     CDS             7656..7949
                     /gene="ybfE"
                     /locus_tag="ACHJ8C_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO5553608.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            8089..8619
                     /gene="fldA"
                     /locus_tag="ACHJ8C_003825"
     CDS             8089..8619
                     /gene="fldA"
                     /locus_tag="ACHJ8C_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO5553609.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8829..8915
                     /gene="uof"
                     /locus_tag="ACHJ8C_003826"
     CDS             8829..8915
                     /gene="uof"
                     /locus_tag="ACHJ8C_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO5553610.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8908..9354
                     /gene="fur"
                     /locus_tag="ACHJ8C_003827"
     CDS             8908..9354
                     /gene="fur"
                     /locus_tag="ACHJ8C_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO5553611.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9438..9764)
                     /gene="chiQ"
                     /locus_tag="ACHJ8C_003828"
     CDS             complement(9438..9764)
                     /gene="chiQ"
                     /locus_tag="ACHJ8C_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO5553612.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9814..11220)
                     /gene="chiP"
                     /locus_tag="ACHJ8C_003829"
     CDS             complement(9814..11220)
                     /gene="chiP"
                     /locus_tag="ACHJ8C_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO5553613.1"
                     /translation="MRAFSGKRSTLALAIAGVTAMSGFMAIPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAVFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDDGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFILEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2828 a         2837 c         2810 g         2971 t
ORIGIN      
        1 ttattaattt attattaaac ctgccccgaa aattattatt tggcaggttt aatttcttaa
       61 tgaaaataca acccacaaaa ttgcaacatt taaacaacaa aacagcatct aatatggcgc
      121 tattcatcat taatgattta ttatttggga tatgatcgtt ttttgttgat cttcttcaca
      181 gattatagcc atttcatgga tagaataacc ctaccttcaa ctgacacaga aaaaggtaaa
      241 ggtaaatgga aaataataac cgcttaatgc ctcatataag gcggacaacc catatcatga
      301 agtttgccca tcgtaatagt tttgactttc atttctttaa tgcccgctag tcttctgact
      361 aaagggcacc ccagcgtaca ggtctccctg actttaagca ttacaggtta atacctgtat
      421 ccctcggtgc tcatatactg ctaacccttt caaactctaa ataattcgag tcgcagcata
      481 ggcaacttga agtatgacga gtatatactc taaataattc gagttgcagg aaggcgacaa
      541 gcgagtgaat ccccaggagc ttacatgagt aagtgactgg ggtgagcgaa cgcagacgca
      601 gcacatgcaa cttgaagtat gacgagtata gccagttacc gggctggtct gggttattgc
      661 atctgcaaaa agcaaactac tgatttattt atcagcggtg gggttttgct tttttccggc
      721 gtgatcaatt tctcctttga gaaattgagg acctgctatt acctgaaata aagagatgaa
      781 caaaatgtca gaattaaaaa ttgccgttag ccgttcttgc ccggattgtt tttccactca
      841 tcgtgaatgc gtgaatattg acaaaagtaa ttatattgac gttgccgcca ttattttatc
      901 agtcaatgat gttgaacgtg gaaaactcga tgaaatagac gctactggct atggcattcc
      961 tgtttttatt gcaacggaaa atgaagaacg tgtaccagca gaatatctgc cacgtatttc
     1021 tggtgtcttc gagcattgcg aatcacgaaa agagttttat ggacgtcagt tagaaaccgc
     1081 tgccagccat tatgaaacgc aactgcgccc gcccttcttc cgcgcactgg tcgattatgt
     1141 caatcagggc aatagcgctt ttgactgccc gggtcatcag ggaggtgagt ttttccgtcg
     1201 ccatccggca ggcaatcagt tcgtggaata ttttggcgaa atgctcttcc gctccgactt
     1261 gtgtaatgcc gacgttgcaa tgggcgatct actgattcat gaaggtgctc catgcattgc
     1321 acagcaacat gcggctaaag tgtttaatgc cgataaaacg tactttgttt taaacggcac
     1381 ctcctcctcc aacaaagtgg ttttaaacgc cctgctgaca ccgggtgatc tggtgctgtt
     1441 tgaccgcaat aaccataagt ctaaccatca cggggccttg ctacaggctg gtgcaacacc
     1501 ggtttatctg gaaacagcac gtaacccgta tggttttatc ggtggcattg atgcgcactg
     1561 cttcgaagaa agttatctgc gtgaactgat tacagaagtt gcaccgcaac gggcaaaaga
     1621 agcgcgtccg ttccgcctcg ctgtgattca gttaggcacc tacgacggta ctatttataa
     1681 cgcccgccag gtggtggata aaatcggtca tctgtgtgac tacatcctgt ttgactcagc
     1741 atgggtcggc tatgaacagt ttattccgat gatggcggac tgttcgccgc tgctgctgga
     1801 gcttaatgag aacgatccgg gtattctggt tacgcaatct gtgcataaac aacaggctgg
     1861 tttttctcag acttcacaaa ttcataaaaa agacagccat atcaaagggc aacagcgtta
     1921 tgtaccgcac aaacgcatga acaacgcctt tatgatgcac gcctccacca gcccgttcta
     1981 tccgctgttt gccgcactgg atatcaacgc caaaatgcat gaaggtgtca gcggtcgtaa
     2041 tatgtggatg gattgtgtgg ttaacggtat taatgcccgc aaattgatcc tcgataactg
     2101 ccagcacatt cgcccgttcg ttcctgaact ggtggatggt aaaccctggc agtcgtatga
     2161 aacagcgcaa attgcggttg atctgcgctt ctttaaattt gtacccggtg agcactggca
     2221 ttcttttgaa ggctacgcag agaaccaata ctttgtcgat ccgtgcaaac tgttgctgac
     2281 aactccaggt attgatgcac gtaacggcga atatgaagcg ttcggtgtac ccgcaacgat
     2341 tcttgctaac ttcctgcgcg aaaatggcgt agtgccggaa aaatgcgatc ttaactccat
     2401 cctcttcctg ctgactccgg cagaagatat ggccaaactg cagcaacttg ttgccctgct
     2461 ggtacgcttc gaaaaactac tggaagctga tgcgcctctg gcggaagtac tgccttccat
     2521 ctacaaacag catgaagagc gctacgccgg ttataccctt cgtcagttgt gccaggaaat
     2581 gcatgatttg tatgcccgcc acaacgtgaa acaactgcaa aaagagatgt tccgtaagga
     2641 acacttccca cgcgtcagca tgaatccgca agaagccaac tacgcctact tacgcggtga
     2701 agtggagctg gttcgtctgc cggatgcaga aggccgtatc gctgccgaag gtgcgcttcc
     2761 ttatcctccg ggtgtgctgt gtgttgttcc tggtgaaatc tggggtggtg ctgttttgcg
     2821 ttacttcagc gctctggaag aaggcatcaa cctgctgcca ggttttgcac cggagctgca
     2881 gggtgtctat atcgaagaac atgatggtcg taagcaagtt tggtgctatg tcatcaagcc
     2941 tcgtgatgcg caaagcaccc tgttgaaagg ggaaaaatta tgagtcaggc gaaatcgaac
     3001 aaaatgggtg tcgttcagtt aaccatactg acgatggtca acatgatggg ctccggtatc
     3061 atcatgctgc cgacaaagct tgccgaagtc gggacaatct caattatctc ctggctggtg
     3121 acagccgtag gctcaatggc actggcatgg gcattcgcaa aatgcggtat gttcagccgt
     3181 aaatcaggag gtatgggcgg ttatgccgag tatgctttcg gtaaatccgg taactttatg
     3241 gcgaactata cctacggcgt ctcgctgctg attgctaacg tcgcgattgc tatttcggcg
     3301 gttggttacg gcaccgaact gctcggcgcg agtttgtcgc cagtgcagat tggtcttgcg
     3361 accatcgggg tgctgtggat ttgtaccgtg gctaactttg gtggtgcgcg cattaccggg
     3421 caaatcagta gcattaccgt gtggggggtc attattccgg tggtaggcct gtgcattatc
     3481 ggctggttct ggtttagccc gacactttac gttgattcct ggaatccgca tcatgcaccg
     3541 ttcttcagtg cggtaggttc ttccatcgct atgacgctgt gggcttttct tggtctggag
     3601 tctgagtgtg cgaatactga tgtagtggaa aacccggaac gtaatgtgcc aatcgcggta
     3661 ctcggcggta cgttaggtgc ggcggtgatt tatatcgtct ccaccaacgt gattgccggg
     3721 attgtgccaa atatggagct ggcaaattca acggcaccat ttggtctggc cttcgcgcag
     3781 atgttcacgc cggaagtggg taaagtcatt atggcgctga tggtgatgtc ctgctgcggt
     3841 tcgctgcttg gctggcagtt caccattgcc caggtgttta aatcttctgc cgatgaaggg
     3901 tacttcccga aaattttctc ccgtgtgagc aaagtcgatg caccagtaca gggaatgctg
     3961 accattgtca tcatccagag cggattatca ctgatgacca ttagcccgtc gctgaacagt
     4021 cagttcaacg tgctggttaa cctggccgtg gtgaccaata tcatcccgta tattctgtca
     4081 atggcggcat tagtcattat tcagaaggtc gctaatgtgc ccccctcaaa agcgaaagtt
     4141 gcaaactttg ttgcttttgt tggcgcgatg tacagctttt atgcgctgta ctcatccggg
     4201 gaagaagcca tgctatacgg ttccatcgtg accttcctcg gttggacact gtatggtctg
     4261 gtctcaccac gctttgaact gaaaaataaa cacggttaat taagcaaagc aaaaaagggc
     4321 gatcttccga ccgccctttt tcttatttga tatgtttacg cgtttttcag aacttcgcta
     4381 acaatctcaa ccgcttcttt ctcaatctgc ttgcgatgtt cttcaccaag gaagctttcg
     4441 cagtagatct tatatgcgtc ttccgtgcct gacggacgcg cggcgaacca gccgttgtca
     4501 gtcatcactt tcagaccgcc gatagaagcg ccattacccg gtgccgccgt cagacgcgcg
     4561 gtgatcgggt cacctgccag ggtgctggcg ctcaccattt ccggagacag cttagacaac
     4621 gccgcttttt gtgcggaagt cgctgctgcc tgcaaacggt tatagctcgg cgcaccaaag
     4681 cgttttgcca gttcgttata gtgttcctgc gggttcttac cggtgacagc ggtgatttcc
     4741 gccgccagca gacacataat gatgccgtct ttatcggtgg accacggcgt gccgtcgaaa
     4801 cgcaggaagg aagcgcctgc actctcttcg ccgccaaagc cgaagctgcc atcgaacaaa
     4861 ccatcaacaa accatttgaa acctaccggt acttctacca gcttacggcc caaatcattg
     4921 accacacggt cgatcatcgc agatgaaacc agcgttttac cgacagcaac atctttgccc
     4981 cactgcggac gatgctggaa caggtagttg atcgccaccg ccaggtagtg gttcggattc
     5041 atcaaacctg ccggagtgac gataccgtga cggtcataat ccgggtcgtt agcaaacgcc
     5101 agatcgaact tatcacgcag tgccagcagg cccgccatcg cacactcgga ggagcagtcc
     5161 atacggatcg caccgtcttt atcgaggtgc ataaagcgga aggtttgatc gacctgatcg
     5221 ttaacgatgg tcaggttgag gttgtaatac tcgccaatgc gcttccagta ttcgataccg
     5281 gaaccaccca gcggatcaac gcccagcgtc aggcccgctt tctgaatcgc cgccatatca
     5341 acgatatcgg ccagcccttc cacaaacggc tgcaccagat cctgctcttt cacatgaccg
     5401 gatgccatcg cttcgtcgag ggagatacgc ttcacgcctt tcaggccatc agccagcagt
     5461 gcgttggctc tgtcttccac cactttagtg acgttggtat cagccgggcc accatttggc
     5521 ggattgtact tgataccacc atcttccggc gggttatggg acggtgtaat cacgataccg
     5581 tctgccagcg ggccaccttt tttattgtga accagaatgg cattggaaat ggcaggtgtc
     5641 ggagtgaagc cattgttttc ctgcacaatg acatcaacgc cgttcgctgc cagcacttcc
     5701 agtacggaaa tgaacgcagg ctcggacagg gcgtgagtat ctttacccac atagcaaggg
     5761 ccagtgatgc cgtttttcgc acgttcttca gcaattgcct gagcgatcgc cagaatgtgc
     5821 ggctcgttaa agctgtggcg cgctgcactg ccacggtgac cggaagtacc gaatttcacc
     5881 gcgtgctccg cattccctgc ttctggtttc agtacataat attgcgccgt cagttgggcg
     5941 acgttaatca aatcactctg ttgtgcaggt tggcctgcac gattgtggat tgccattgct
     6001 ttgtcctttg tctgcaacgt tttagatagt tccgcaaacc ttctcaatca attccgccgg
     6061 gaattgcatc gactgcatga tgtgttcgat catgctgcat ttacggccgg tgttggtgtt
     6121 ggtgatcacc caatacggcg taccaggcac atgtttcggc ttggtctgat taccattttt
     6181 cagcagcgtt tgttcatctg ccgcaaagta aacgcgtgta cgaccgtgca acgattccgt
     6241 tgcttcggca aacgcctggg cgtcaagaga atatagtgta gacaacagca gcataaagcg
     6301 attgaccgct cgcttttgct ctgcgtattc gtccgaaagc agaagttcac gcattgcgcg
     6361 aaccttgtct ttaatcgttt tgaccggctt cgcttcgacg atagcaggtg acgcaacgcg
     6421 aacctctttc gtcaccggag cagcaggctg tgatgcggcg gaaaatttca acatacgccg
     6481 taaaatgtcg gatgcgctct cgccgatatg cttagtgtgg ctggcaatat agctgtagag
     6541 ttcatcatca acttcaatcg ttttcatctt aatccagtgc agtgtcttag ctgaatacaa
     6601 gtcgccagga gtatatgaat aaatcccggc agcggatagc gtcaagcctg gcttgcagaa
     6661 aaactcggaa taaaccttat ttgttgcagc cctgtggcac aatggtcaac ataataccct
     6721 aacccgacaa cgcgaaaaaa agaactttgc catgaaattg aatatccgcg cgcaaactgc
     6781 acaaaaccag cacaataatt ctcccatcgt tcttgtccat ggtctgtttg gcagcctcga
     6841 caaccttggc atactggctc gcgatctggt aaacgatcac aatatcatcc aggttgatat
     6901 gcgtaaccac ggtctttcac cgagagatcc ggtaatgaat tacccggcga tggcgcagga
     6961 tcttgttgat accctggatg cacagcagat cgacaaagca acatttatcg gtcactccat
     7021 gggtggtaaa gcggtaatgg ctcttactgc actagccccc gatcgcattg acaagctggt
     7081 ggcgattgat attgcgccgg tcgattatca cgttcgccgt catgatgaga ttttcgcggc
     7141 catcaacgca gtcagtgaat cagacgcaca aacacgccag caagcagcgg caataatgcg
     7201 ccagcatctt aatgaagaag gggtgattca gtttctgttg aaatcttttg ttgacgggga
     7261 gtggcgcttt aacgtgccag tattgtggga tcagtatccg catattgtgg gttgggagaa
     7321 aatcccggca tgggatcatc ctaccctgtt tatccctggc ggcaattctc cgtatgttag
     7381 cgagcagtac cgtgatgatt tactggcaca atttccacag gctcgggcgc atgtgattgc
     7441 aggcgcgggt cactgggtcc atgctgaaaa accggatgcg gtattacgcg ctatccgtcg
     7501 ctatctcaat gattaacgca actgattaaa aacccagcgt ccgcgtcgct ttccgcgcgt
     7561 ggacgttggc gtgactcatc gcgctgatgt attatggcgc gctatccatc cgggcaggcg
     7621 cctggctgat tgtttccccc gaagtcacca agatcatggc caaagaacaa acggaccgta
     7681 cgacattaga tctgttcgcg cacgagcgtc gaccgggacg accgaaaact aatccgcttt
     7741 cgcgcgatga gcagctgcgt attaataaac gcaaccaact aaaacgcgac aaagtacgtg
     7801 gccttaagcg tgtcgaactg aagctgaacg cggaagctgt cgaggcactg aacgagctgg
     7861 cggaatcgcg caatatgagc cgtagcgagc tgatcgaaga gatgttaatg cagcaactgg
     7921 cggcgctgcg tagtcagggc atcgtttaaa tttccacttt catgtagcac agtgtgcagt
     7981 cctgctcgtt tgctgattcc gcaagactgc ctgttctgct atgattgcct ttatccgtgg
     8041 gcaattttcc acccccattt caataagttt caagaggtta tttcactcat ggctatcact
     8101 ggcatctttt tcggcagcga caccggtaat accgaaaata tcgcaaaaat gattcaaaaa
     8161 cagcttggta aagacgttgc cgatgtccat gacattgcaa aaagcagcaa agaagatctg
     8221 gaagcatatg acattctgct gctgggcatc ccaacctggt attacggcga agcgcagtgt
     8281 gactgggatg acttcttccc gactctcgaa gagattgatt tcaatggtaa actggttgcg
     8341 ctgtttggtt gtggcgacca ggaagattac gcagaatact tctgcgatgc attgggcacc
     8401 atccgcgaca tcattgaacc gcgcggcgca actatcgttg gtcactggcc aaccgcgggc
     8461 taccacttcg aagcgtcaaa aggtctggct gatgatgacc actttgtcgg tctggctatc
     8521 gacgaagatc gtcagccgga actgaccgct gaacgtgtag aaaaatgggt taaacaaatt
     8581 tctgaagagc tgcatctcga cgaaattctc aatgcctgat gtgatgcggc gtagactcat
     8641 gtctacgccg tattaataga taatgccaat caaaataatt gctacaaatt tgtaactttt
     8701 gctgttgtac ctgtacaatg tcccggtgtt caagtggcct tgccgttgta aatgtaagct
     8761 gtgccacgtt tttattaaca atatttgcca gggacttgtg gttttcattt aggcgtggca
     8821 attctataat gatacgcatt atctcaagag caaattctgt cacttcttct aatgaagtga
     8881 accgcttagt aacaggacag attccgcatg actgataaca ataccgccct aaagaaagct
     8941 ggcctgaaag taacgcttcc tcgcttgaaa atcctggaag ttcttcagga gccggacaac
     9001 catcacgtca gtgcggaaga tttatacaaa cgtctgatcg atatgggtga agaaattggt
     9061 ctggctacgg tatatcgcgt actgaaccag tttgacgacg ctggtatcgt cacccgccac
     9121 aattttgaag gcggtaaatc cgtatttgaa ctaacacagc aacatcacca cgatcacctg
     9181 atctgcctcg actgcggtaa ggttatcgaa tttagtgatg attccatcga agcgcgtcag
     9241 cgtgaaatcg ccgcaaaaca tggcattcgc ctgactaacc acagtctcta tctttacggt
     9301 cactgtgccg aaggcgattg ccgcgaagat gagcacgcgc acgaaggcaa ataagccagc
     9361 ctgaacgaga aaagccaacc tgcaggttgg cttttttata caagggaaat aagaaaagcg
     9421 agagttacag ctctcactta tttgttatta ctgcgaattt cctgccagac cttatcacaa
     9481 tcggctttca ccgcctggtc attaccggtt tgcgttgcgc gcagacactg ctgataatcc
     9541 agtacgcgta cactttcctg gtcagcaaac tgctgatgtt gtttctcttt cttcagcacg
     9601 tttaacacac tctggcaggc ttcaattttt tccggcgaac cttgcgcggt gttgatacag
     9661 gcgctgtaag cctctttcag acgactgtct tcctgcggtg cggttgattg cgcacaagct
     9721 accagccccg atgccatcat ggcgatgaga atcagttttt tcatagcaaa ttcctcaaac
     9781 gtgcggcagg cacaaaacct gccgcgtcgg gcatcagaag atggtgaatg gtgcgattac
     9841 catgaatttc acgtcgcgct catcctggaa aatgttgccg taaccaccgc cccagcttgg
     9901 gatgtcggag tggttgtcat attcggtgaa atgcagtttg aacatcgtgc ccttggcacg
     9961 accgtcctga atggtgtaaa ccgcatccag gctgtaggca gactcttcaa tagtccggtt
    10021 cttgtcgtag tacgcatccg gattgctctg ccaggtcgca ggtttagcat cccatgcgta
    10081 aacgtaggaa gcgccgatgg cgaagcctgg aagattccag tttttcaggt catacatcgc
    10141 accgaagaag accgcttttt cgccgttggc gttgaagtcg gaacggttat cccaccagat
    10201 atccaggcga ccgttcgagg aagcgtaggt tggagtcata cgttgcagga agtatccttg
    10261 ctgaccgtca gccttaaccc aggtaccttc gaggcgcaaa tccactacgt cagccgcccg
    10321 gtaaccaaag gtcaacgcct gcagccaggc ggtgccgtca taaagatcgt tgacgctgcg
    10381 atcgtcaact ttatcgcgcg taccatagaa ctggtagctg gtggttaacg ggctaccggc
    10441 gatatcaaat ttgtagctgg ctttggcaaa atattgatcg atataccctt cggcctgacc
    10501 aaacgccgct tccagtatga agttattttt gaagtcgtac ttcgccccaa gggagtgtag
    10561 ataatcaact ttggtggttt tatcgttctg ataaaactca tccatttcca gatgccacgg
    10621 tgctttgtat tcgttggtcc acatgtagga gaaactcaac gcaccagcat cgccgtcatc
    10681 aaaattcgcc ccggcctccg ccccctgata agtacccggc ataaagctcc agtgtggcgc
    10741 taacagcgtc tgaccggttg gctgaatata acctgccctc gcccaaaccg gaccgtattt
    10801 aaatttggcc gctgctttat acaggcttat accgctttta tcgccggacc agtcttcgtc
    10861 ataggcttta ttactttttg aaaacgcgat ttcgttcggg tgagagctgt cgccgttttc
    10921 cgccatttca atcgccgtaa acacggcaat atcaaggccg aacatatcag cagcataacc
    10981 agactgaaaa tcgaggttgg cgttccaggt ggagtgagaa aggttggttt tgtatttatc
    11041 gccgtcggta acatctttac ggtcgcgttc acgctgccag taatagatac cgccggttaa
    11101 ggttgaatcg tcgatgaatc cttcggcgcg agcctccgga attgccataa agcccgacat
    11161 agctgtaaca ccggcgatag ccagcgccag cgtactacgt ttgccactaa acgcacgcat
    11221 gggttaatcc tctttgacgt ataaattgct gcaccaaagg tgaacagcga aaaaaataaa
    11281 aattaaaaaa gtagcgttga gaaaagtctc gctgactaca ggaacagact atttttcgcg
    11341 acgcgaatta tcaatctctt tctgtaacca gaatgtaaga acatgagcaa ccgcacatat
    11401 tttgatgatt tttgttacaa ggcttaagtt tgcagttgaa aggctt
//
LOCUS       DAZFJL010000137        11439 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFJL010000137 DAZFJL010000000
VERSION     DAZFJL010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.139"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            8..610
                     /gene="yebB"
                     /locus_tag="ACHJ8C_003830"
     CDS             8..610
                     /gene="yebB"
                     /locus_tag="ACHJ8C_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO5553614.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAVGVETPQPELTEAV"
     gene            complement(612..1133)
                     /gene="ruvC"
                     /locus_tag="ACHJ8C_003831"
     CDS             complement(612..1133)
                     /gene="ruvC"
                     /locus_tag="ACHJ8C_003831"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO5553615.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1168..1908)
                     /gene="yebC"
                     /locus_tag="ACHJ8C_003832"
     CDS             complement(1168..1908)
                     /gene="yebC"
                     /locus_tag="ACHJ8C_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5553616.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRIVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1937..2389)
                     /gene="nudB"
                     /locus_tag="ACHJ8C_003833"
     CDS             complement(1937..2389)
                     /gene="nudB"
                     /locus_tag="ACHJ8C_003833"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO5553617.1"
                     /translation="MTDMAYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDASAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2382..4154)
                     /gene="aspS"
                     /locus_tag="ACHJ8C_003834"
     CDS             complement(2382..4154)
                     /gene="aspS"
                     /locus_tag="ACHJ8C_003834"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO5553618.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGYFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILERTGAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4464..5030
                     /gene="yecD"
                     /locus_tag="ACHJ8C_003835"
     CDS             4464..5030
                     /gene="yecD"
                     /locus_tag="ACHJ8C_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO5553619.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RANGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVKEILNAL"
     gene            5027..5845
                     /gene="yecE"
                     /locus_tag="ACHJ8C_003836"
     CDS             5027..5845
                     /gene="yecE"
                     /locus_tag="ACHJ8C_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO5553620.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTAKNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            5898..6293
                     /gene="yecN"
                     /locus_tag="ACHJ8C_003837"
     CDS             5898..6293
                     /gene="yecN"
                     /locus_tag="ACHJ8C_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO5553621.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6334..7077
                     /gene="cmoA"
                     /locus_tag="ACHJ8C_003838"
     CDS             6334..7077
                     /gene="cmoA"
                     /locus_tag="ACHJ8C_003838"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO5553622.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7074..8045
                     /gene="cmoB"
                     /locus_tag="ACHJ8C_003839"
     CDS             7074..8045
                     /gene="cmoB"
                     /locus_tag="ACHJ8C_003839"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO5553623.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(8210..10639)
                     /gene="torZ"
                     /locus_tag="ACHJ8C_003840"
     CDS             complement(8210..10639)
                     /gene="torZ"
                     /locus_tag="ACHJ8C_003840"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO5553624.1"
                     /translation="MTLTRREFIKHSGIAAGTLVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPV
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAAQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYIRYGDFRADPVK
                     NALGTPSGKIEIYSRTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHKGAWPDLENGLCKNGSANVLTADISSSQLANACAGNSALVYIEKYTGNALK
                     LTAFDQPTVQA"
     gene            complement(10664..>11439)
                     /locus_tag="ACHJ8C_003841"
     CDS             complement(10664..>11439)
                     /locus_tag="ACHJ8C_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO5553625.1"
                     /translation="RQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNK
                     AQKDGETCIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELA
                     TVTPGTDLTVVDASGKQPIVRLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVN
                     GEWQADEYGNQWRQASLQGALIDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTV
                     NTWPSIAKGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2577 a         3044 c         2840 g         2978 t
ORIGIN      
        1 tgacaacgtg aatattaatt atcctgctga atatgaaatt ggtgatatcg tctttacatg
       61 tataggtgct gccttatttg gtcaaatatc agctgcatca aattgctgga gtaatcacgt
      121 cgggatcatt atcggtcata acggtgaaga ctttctggtt gcagaaagcc gtgttcccct
      181 ctcaaccatc actacgctat cccgttttat taaacgctct gctaatcaac gctatgctat
      241 aaagcgatta gacgccggac taacagaaca acaaaaacaa cgaattgttg aacaggttcc
      301 ttcccggcta cgcaaacttt accacaccgg ttttaaatac gaatcttcgc gccagttctg
      361 ttcaaaattt gtttttgata tttataaaga ggcgctatgt attccggtgg gtgaaataga
      421 gacgtttgga gaattgttaa atagcaatcc gaatgcaaaa ctcactttct ggaaattctg
      481 gttcttaggt tctattccgt gggagcgtaa aaccgtcacg ccagccagtt tgtggcatca
      541 tccgggtttg gtgttgattc acgcggtggg agttgaaacg cctcagcctg aactgaccga
      601 ggcggtataa cttaacgcag tcgccctctc gccaggttca gtcgcgattc gctcatttgc
      661 atcgcattct gactaacgtg gcagtgggtg atggcaatcg ccagcgcatc ggcggcatcc
      721 gcctgtggat tagcgggcag tttcagcaag gtgcggacca tatgctgcac ctggcttttt
      781 tcggcactac caatacctac cactgtttgc tttacctgac gtgccgcata ttcaaatacc
      841 ggcaattcct gattcaccgc cgccacaatc gccacgccgc gcgcctgccc cagtttcagg
      901 gctgagtcag cgttcttcgc cataaagacc tgttcaatgg cgaaataatc aggctggaat
      961 tgggtgatga tttccgtcac gcccgcatag atgagcttca gacgagacgg taaatcatcc
     1021 actttggtgc gtatgcatcc gctacccagg taggacagtt gcctgcctac ctggcggatg
     1081 acgccatagc cggtcacgcg cgaacccggg tcaatgccga gaataatagc catcacgcgt
     1141 ctccgttttg ctgtttagca ggcctcatca gagagtcgct gcaacctcat cagagatttc
     1201 accgttatgg taaacttcct gcacgtcgtc gcaatcctcc agcatatcga tcagacgcat
     1261 cagtttcggt gcggtttctg catccatatc agctttggtg gacgggatca tggaaacttc
     1321 cgcgctgtct gctttcagac ctgccgcttc cagagcgtcg cgtactttgc ccatttcttc
     1381 ccatgcagtg tagacatcaa tcgcgccgtc atcataggtc acaacgtctt cagcaccggc
     1441 ttccagggct gcttccatga tggtgtcttc atcgcctttc tcgaaggaga tcacgccttt
     1501 tttgctgaac aaataagcta cggaaccatc agtaccgagg ttaccgccac atttgctgaa
     1561 tgcatgacgc acttcagcaa cgatacggtt gcggttgtca gacagacatt caatcatgat
     1621 tgccgtgccg ccaggaccgt aaccttcgta gatgatggtt tccatgtttg catcatcatc
     1681 accgcccaca cctcgtgcaa ttgcgcggtt cagagtgtca cgggtcatgt tgttagacag
     1741 tgctttatca attgctgcac gcagacgcgg gttagcgtcc ggatcaccac cgcccagctt
     1801 agccgcggtt accagctcac gaatgatttt agtgaagatt ttaccgcgct tagcatcctg
     1861 cgcagcttta cgatgtctgg tgttggccca tttactatga cctgccataa aaatatctcc
     1921 agatagccct gcctgttcag gcagcgttaa ttacaaactg ttcaatcgcc tgccggttgc
     1981 tccaggactt agtgagcgcc gccgcagcag acgcatcaag ccacttgtaa gccagatgtt
     2041 cagtgaaaac gatctggcgc tcgtgcggaa gcgcaagaca gaaccatgat tccgtattac
     2101 gcgtcacgcc cggcgcatag cgatgacgta aatgtgaaaa aatttcaaac tctaccgtgc
     2161 gctgacagtc aattaaggtc agttgttcag cgacaacatc aatggtgacc tcttccttta
     2221 cttcgcgcat ggcagcttgc ggcgcggttt ccccctcttc cacgctgccg gttaccgact
     2281 gccagaaatc gggatcgtca cgccgctgca acatcagcac ccgtttcgta tcttgtgcgt
     2341 agatgaccac taagatcgaa acgggacgct tataagccat atcagttatt ctcagccttc
     2401 ttcacaacct gaatgctcag ctcagccagt gcagtcgggt tagcaaagct cggtgcttca
     2461 gtcatcagac acgctgccgc cgtggttttc gggaaggcga taacgtcacg gatattgtcg
     2521 gtgccggtca gcagcatcgt cagacggtca agaccgaatg ccaaacctgc gtgcggcgga
     2581 gtaccgtatt tcagggcgtc gagcaggaag ccgaatttct cgcgctgttc ctcttcgttg
     2641 atacccagaa taccaaacac cgtctgctgc atatcaccat tatggatacg cacagaacca
     2701 ccgcccactt cgtaaccatt gattaccata tcgtaagcgt tagccaccgc attttccggt
     2761 gcagctttca gttctgctgc cgtcatgtct ttcggtgagg tgaacggatg gtgcattgcc
     2821 gtcaggccgc cttcaccgtc gtcttcaaac atcgggaagt cgataaccca cagcggtgcc
     2881 catttgcttt cgtcggtcag accaaggtct ttacccactt tcaggcgcag tgcccccatc
     2941 gcgtcggcaa caattttctt gttgtcggca ccgaagaaaa tcatatcgcc atcttgcgcg
     3001 ccagtacgct ccaggatggc ttcaatgatt tctgcattga ggaacttcgc taccgggctg
     3061 ttgatacctt ccagaccttt cgcgcgttcg ttaactttga tgtaagccag acctttcgcg
     3121 ccgtagattt taacgaagtt accgtattcg tcgatctgct tacgggtcaa cgatgcgccg
     3181 cccggaacac gcagagcggc aacacggcct ttcggatcgt tcgccggacc tgcaaatact
     3241 gcaaactcaa cagatttaag cagatcggca acgtcggtca gttccatcgg gttacgcaga
     3301 tccggtttat cagaaccata acggcgttct gcttctgcaa aggtcattac cgggaaatag
     3361 cccagatcca cacccttcac ttccagccac agatgacgca ccagcgcttc catcacttca
     3421 cgcacttgcg gcgcggtcat gaaagaagtt tccacatcga tctgagtaaa ttcaggctga
     3481 cggtcagcac gcaggtcttc gtcacggaag catttaacga tctgatagta gcggtcaaag
     3541 ccggacatca tcagtagctg tttgaacaac tgcggggatt gcggcagcgc gtagaattta
     3601 cctttgtgca cacgagaagg caccaggtag tcacgcgcgc cttcaggcgt ggctttggtc
     3661 agcatcggag tttcgatgtc gaggaagccg tggtcatcca taaaacggcg caccaggctg
     3721 gtgattttag cgcgggtttt caggcgctga gccatttccg ggcgacgcag gtcgaggtag
     3781 cggtatttca gacgcgcttc ttcggtgttg acgtggttag agtcaagcgg cagaacatct
     3841 gcacggttga tgatagtcag cgaggacgcc agtacttcga tttcgccagt cgccatatcg
     3901 cggttaatat ttttttcatc acgcgcacgt acggtgcccg tgacctgaat gcagaactca
     3961 ttacgcagtt cagaggccag ctttaacgcg tccgcacgat ccggatcgaa aaatacctgc
     4021 acgatacctt cgcggtcgcg catatcgatg aagatcaggc taccaagatc acgacgacgg
     4081 ttgacccaac cacacagagt cacctgctgc cccacgtggg acaaacggag ctgtccacaa
     4141 tattctgtac gcatgagata tcccttaact tagctgccgg cggatgcccc ctgctgcgca
     4201 ggtgaccaag tcgcagcgtt agctgtatgt cacaactgaa tgaaaaaagg cggctattat
     4261 actggaaatt ctgccgcacc gtaagagcct ggcccgcgct ggaacgcctc gttaccactt
     4321 tatatcgggc ctgaaatcag actctacgcc agtttgctat aaaggtgttg cccgaactca
     4381 taaaaattaa caaaatttgt cgttccgcca tcggctaatc gcattaaggt gagaggcacg
     4441 attttgtttt gtcaggagtc atcatgcttg aacttaatgc taaaaccacc gcgctggtgg
     4501 tgattgattt acaagaaggc atcttgcctt ttgccggtgg tccgcatact gccgatgagg
     4561 tggttaatcg ggcagggaag ctggcggcga aatttcgcgc gaacggtcag cccgtgtttc
     4621 tggtgcgcgt tggttggtct gccgattacg ccgaagcatt aaaacagccg gttgatgccc
     4681 cctcccccgc aaaagtgttg cccgaaaact ggtggcaaca tcctgctgca ttaggtgcaa
     4741 ccgacagcga tatcgaaatc atcaaacgtc aatggggtgc gttttacggt acggatctgg
     4801 agttgcaatt acgccgccgg ggtatcgata caatagtgtt atgtgggatc tcgaccaata
     4861 tcggtgttga atccaccgcc cgcaatgcct gggaactcgg ttttaatctg gtgattgccg
     4921 aagatgcctg tagcgccgct agcgccgagc agcacaataa cagcattaat catatctacc
     4981 cgcgtatcgc ccgtgtgcgt agcgttaaag agatcctcaa cgcgttatga tttacatcgg
     5041 tttgccacaa tggtcgcatc ctaaatgggt gcggttgggg atcaccagcc ttgaagagta
     5101 tgcccgccac tttaactgtg tggagggtaa caccacgctt tatgccctgc cgaaacccga
     5161 ggttgtcctg cgctggcgtg agcagaccac agatgacttc cgcttctgtt ttaagtttcc
     5221 ggcgaccatt tcgcatcagg cagcattacg gcattgcgat gatttagtga ctgaattttt
     5281 gacccgcatg tcaccgttgg ctccgcgcat tggacaatac tggctgcaac tgcctgccac
     5341 attcggccca cgggagctgc ctgcgctttg gcattttctc gattctcttc ccggtgaatt
     5401 taattatggg gtggaagtcc gccatccaca gtttttcgcc aaaggggaag aggaacaaac
     5461 gcttaatcgc ggtttacatc agcgcggcgt taatcgggtg attttagaca gccgcccggt
     5521 tcatgcagca cgtccacaca gtgaagctat tcgcgacgct caacgaaaaa aacctaaagt
     5581 tccggtacat gctgtactga cggcgaaaaa tccactgatc cgttttatcg gtagtgatga
     5641 tatgacgcaa aaccgggaat tatttcaggt ctggttacaa aaattagcac agtggcatca
     5701 gaccactacg ccttatcttt ttttacatac gccagatatt gcccaggccc cggaactggt
     5761 acataccctg tgggaagact tacgtaaaac gcttccagag atcggagcag ttccggctat
     5821 tccacagcaa tcttctcttt tctgaatttg ccacctatca tagacaggtg ccatcggcca
     5881 ttttaaaggg agtttgtatg gtaagcgcgc tgtatgccgt tttaagtgcg ttgttattaa
     5941 tgaagttctc ttttgatgtc gttcgcctgc gaatgcagta ccgcgttgcc tatggcgacg
     6001 gcggttttag cgaactgcaa agcgctattc gcattcatgg taacgcggtg gaatatattc
     6061 ctatcgcgat tgtgttgatg ctgtttatgg aaatgaatgg cgcagaaacc tggatggtgc
     6121 atatttgcgg catcgttttg cttgctggtc gtctgatgca ttattacggt tttcatcacc
     6181 gtctgttccg ctggcgacgt tctggcatga gcgcgacctg gtgtgcgctg ttgctgatgg
     6241 tgctggcgaa tctttggtat atgccctggg agttggtttt ctccctgcgt tagcgcacaa
     6301 tacgccactt tctttttccc ggatttttac gttatgtctc accgcgacac gctattttct
     6361 gcccctatcg ccagactggg cgactggacc tttgatgaac gggtagctga agtcttcccg
     6421 gatatgatcc agcgttccgt tcccggctat tccaatatta tttccatgat tggtatgtta
     6481 gccgagcgct tcgttcaacc tggtacgcag gtttacgatc tgggttgttc tctgggcgcg
     6541 gcgacgctct cggtgcgtcg caacattcat catgataatt gcaaaattat tgccatcgac
     6601 aactccccgg cgatgattga acgctgccgt cgtcatattg acgcctataa agcccctacg
     6661 ccagtagacg ttattgaagg tgatattcgc gatatcgcca ttgaaaacgc atcgatggtg
     6721 gtgctgaatt ttaccctgca attcctggaa ccttccgagc gccaggcgtt actggataaa
     6781 atttatcaag ggctgaaccc gggcggtgcg ctggtgcttt cggaaaaatt cagtttcgaa
     6841 gatgccaaag ttggtgaact gctgttcaac atgcaccacg actttaaacg tgccaacggt
     6901 tacagcgaac tggagatcag ccagaaacgc agcatgctgg aaaacgtgat gctgaccgat
     6961 tccgtggaaa cccataaagc acgcctgcat aaagccggtt ttgagcatag cgagctgtgg
     7021 ttccagtgct ttaactttgg ttcactggtg gcattaaaag cagaggacgc tgcatgatcg
     7081 actttggtaa cttttattct ctgattgcca aaaatcatct ttcacactgg ctcgaaacgc
     7141 tgcccgcgca gattgctaac tggcagcgcg agcagcagca cgggctgttt aagcagtggt
     7201 ccaacgcggt ggaatttctg cctgaaatta aaccgtatcg tctggattta ttgcatagcg
     7261 taaccgccga aagcgaagag ccactgagcg ccgggcaaat taagcgcatt gaaacgctga
     7321 tgcgcaacct gatgccgtgg cgcaaagggc cgttctcact gtatggcgtc aacatcgata
     7381 ccgaatggcg ttccgactgg aaatgggatc gcgttctgcc ccatctttct gatttaaccg
     7441 ggcgcaccat tcttgatgtt ggctgcggga gcggttatca catgtggcgc atgattggcg
     7501 caggcgcgca cctcgcggtg gggatcgacc ctacgcaact gtttctgtgc cagtttgaag
     7561 cggtgcgtaa actactgggt aacgatcagc gcgcacattt gttaccgtta ggtattgaac
     7621 aacttccggc actgaaagcc tttgataccg tcttttcgat gggcgtgctt tatcatcgtc
     7681 gttcaccgct ggagcatctc tggcagttaa aagaccaact ggtgaatgaa ggcgaactgg
     7741 tgctggaaac gctggttatt gatggcgacg aaaacacggt gctggtaccg ggcgatcgtt
     7801 acgctcaaat gcgtaatgtc tatttcattc cttccgcgct ggcgctgaaa aactggctga
     7861 agaagtgtgg ttttgttgat attcgcattg cagatgtgag cgttaccacc acagaagagc
     7921 agcgacgcac cgaatggatg gtcaccgagt ctctggccga ttttctcgac ccgcatgatc
     7981 cgggtaaaac ggtggaaggt tatcctgcgc ctaaacgcgc ggtgctgatt gcgcgcaagc
     8041 cgtaaaggtc tggtaatact gccggatgcg gcgtgaacgc cttatccggc ctacaaagtc
     8101 ttgctaattc aatatattgc aggggctatg taggcctgat aagcatagcg catcaggcag
     8161 ttttgcgttt gcataacctc agcgcccgtt tccgggcgct tttcacgtct tatgcctgaa
     8221 cagttggctg atcaaacgcc gttaacttca gcgcattgcc cgtatatttt tcgatataca
     8281 ccagcgcaga gttaccggca caggcattcg ccagctgcga gctggagata tccgccgtta
     8341 acacgttcgc actaccgttt ttacacaagc cattttccag atccggccat gcacctttat
     8401 gcacgcatac cacgcctttt ttgatcccgt cagtcaccac agcgcctgtc aggatctgac
     8461 cgcgcttgtt ccagacacgc accagatcgc cattcgcaat accaaagcga gcagcatctt
     8521 cggtgtgaat agtgattggt tcacgatcgg caaccgcata ttttttacgc agttccgcat
     8581 agttaagctg actgtgtaaa cggtgtgccg gatgtgcggt cagaagctgc aactgcttct
     8641 cgtcggcggt acccttccac tcatcaggcg caagccaggt tgggtgtgcc gggcaatcct
     8701 tatagccaaa tttttccagc gttctggagt aaatctcaat tttgccgctt ggtgtaccca
     8761 gcgcattttt caccggatcg gcgcggaaat caccataacg aatgtactgt tcgttcttct
     8821 cgctgcggcg catttcgatc agtttatttt gctgccagaa ggcattaaac atcggcatag
     8881 tcacgcgttg cgcacgggca cctttctgag cggcatcata gaaaaatttc agccacgcca
     8941 tttcatcttt accttcggta tagatctctt ttccgccagg tttgagtaat tcagcaagat
     9001 cggcaaacac gtcaaaatcg ttgcgcgctt caaattgcgc agcgacagcc tgcttcatcg
     9061 gcacaatatg ctggttgctg taatcaccgg tcatcgtcag gtcattgcgc tcaaacgatg
     9121 tggtgatcgg taatacgata tctgcgtgtt tggctgctgc ggtccagtag cattcagaaa
     9181 cgacgatcat ctccggtttc tgccatgctt taatcagacg attagtatcc tggtgatggg
     9241 taaagttacc gccacccgcc caccagatca ttttgatatt tgggtacgtt tgctctttac
     9301 cgttatgctg atatttgccg ccaggatttt ccagcgcatc gacgatacgg gcaacaggaa
     9361 tcgccgtcat tccgccatca tcagcggcct cgctggcctg tccggcaatc gccgcagaca
     9421 tttctggtag cacgccgcca acacgcgtcg ggttgccacc gttggagtaa tgataagaga
     9481 aaccaaaccc gccgcccggt gtaccaatct gccctaacat cgccgccagc gtcaccagca
     9541 tccagtgttt ctgttcacca tattgctggc gctgaattcc ccagcctgcc atcagcattg
     9601 tgcgatttgc cgccatcagt tcagcgagtt tgactatctg cgcttccggt acgccagtaa
     9661 tttccgccgc ccaggccgcg ctcttcggcg tattatcgct cttacctgtc agatactctt
     9721 caaactgcgg atacccggta gtgtattttt caaggaacac tttatcgtgt ttgccttgtg
     9781 tcatcagggt atgcgcaatc cctaacatca gtgccacatc ggtgcccata ttcggtgcga
     9841 tccaggtggc attatcgcca aagaattcga tggtttcgga gcggatagga tcaatggcaa
     9901 tcactggttt gccagatttt ttcagctgat ggaagtattc cagcccttgc tcatcggtac
     9961 tgctccaggc aattttgagc gtgttcagcg ggttcatccc ccacagcacc accacctggc
    10021 tgttttccag aataagcggc caggaggtct gctgttcata cacctctaca gaaccgacca
    10081 catgcggcat gatcacctgt gccgctccgg ttgaataatc gccgctatgc ccggaataac
    10141 cgcccgccag gttcatataa cgttgcagta aggtttgcgc tttatgcaac acgccagaag
    10201 aacgccagcc ataagatccg gcaaaaatgg ccgatggtcc gttagctttg cgaatgcgat
    10261 cgtgttgttc atgaatcagt tttaatgctt gttcccagct cacctgtaca taggtatctt
    10321 cgccacgacc tttcaccggt tgcagtggat tatcgagata gctttttctc accatcggat
    10381 gctgaatacg cgccgtggtg tgtacctgat ccgccgccgt agactgtaag gagttcggta
    10441 tggttttcgc cagcgcgcct gtcgaagaaa caatcttgcc gtccttcact tctacgttca
    10501 tcgctcccca acggcccgca gtgagaattt tgccgccctt ctcttctgcc catgcgggta
    10561 gcggtgcagc cgatgtcacc accagcgtcc ctgccgcaat accgctatgt ttaataaatt
    10621 cacgtcttgt taatgtcata acttcctccc tgatcaacga ggatcactgt ttctcggtaa
    10681 tatctttggc gttgtactgg aagtaccgcg tcaaaatgtc cagttcgttt tcgctcatgc
    10741 tggttcgcgc acccattcct ttggcaatgg acggccatgt attgacggtg taatggtcgg
    10801 cggcaatagg ggcatggcag ccagcgcaat aggtatcgtc aagtttttca gcgtattgcc
    10861 atagcggttt acggtccgct aatgcgggat cgataagcgc cccctgcaaa gacgcctgac
    10921 gccattgatt gccgtattcg tcagcctgcc attccccgtt taccgttagc gccttgatac
    10981 cttcttcact taatgtggcg agcgccagcc gttggcctgc cgccaggtag agcgtgtttt
    11041 cactgccctg catttgataa ccctgcaacc gaacaattgg ctgtttgcca ctggcatcaa
    11101 cgacggtaag atcggtacca ggagtcacgg tagccagttc gcctatgcga gaagttttga
    11161 aaggataaat atgtgcgcca ttagtaactg aagtagcggc ctgactttcc agctcatgcg
    11221 ccgcgttgtc atccattttt atttctggcg gaaaatgggc aatgccttta tggcaatcga
    11281 tacaggtttc gccgtccttt tgcgctttat tatgcatttt ctgcgcagat tcactttgcg
    11341 aggcaatatc catggcatca aaagaatggc aactacggca ggttgcagag tcagttgctt
    11401 ttaattcttt ccatactgtt tcggccattt cctggcgat
//
LOCUS       DAZFJL010000138        11416 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZFJL010000138 DAZFJL010000000
VERSION     DAZFJL010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.140"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(12..797)
                     /locus_tag="ACHJ8C_003842"
     CDS             complement(12..797)
                     /locus_tag="ACHJ8C_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5553626.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            1115..2392
                     /locus_tag="ACHJ8C_003843"
     CDS             1115..2392
                     /locus_tag="ACHJ8C_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO5553627.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIIFYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRNTNNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVVQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGYWQDTLPAAEAYRNMWL
                     MGMAALGMVIIFTFLLFQKIRTADSAPAMASSK"
     gene            2419..3897
                     /locus_tag="ACHJ8C_003844"
     CDS             2419..3897
                     /locus_tag="ACHJ8C_003844"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO5553628.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRQMLFDVQMPGTILGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCHEMNHFAKHVIITGGGSNSDLLMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
     gene            4062..4982
                     /locus_tag="ACHJ8C_003845"
     CDS             4062..4982
                     /locus_tag="ACHJ8C_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO5553629.1"
                     /translation="MSARVWVLGDAVVDLLPESQGRLLQCPGGAPANVAVGIARLGGK
                     SAFIGKVGDDPFGRFMYQTLSTENVDTRYMSLDPQQRTSIVAVGLDEQGERNFTFMVR
                     PSADLFLQPGDLPAFGPGEWLHLCSIALSAEPSRSTAFLAMEKIRQAGGNISFDPNIR
                     SDLWQSEALLRKYLDRALSLANIAKLSEEELLFISGESQVQQGAYSLVQRYSLTLLLI
                     TQGKNGVLVYFQGQFIHYPAKPVSVVDTTGAGDAFVAGLLAGLADSGIPTNTRQLERI
                     IAQAQICGALATTAKGAMTALPRQHDLPSQ"
     gene            5150..6667
                     /locus_tag="ACHJ8C_003846"
     CDS             5150..6667
                     /locus_tag="ACHJ8C_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO5553630.1"
                     /translation="MYKKTTLAVLIALLTGATTVHAQTDISSIESRLAALEQRLKNAE
                     SRAQAAEARAKTAELQVQKLAETQQQNQLTTQEVAQRTVQLEQKSAENSGFEFHGYAR
                     SGLLMNDAASSSKSGPYLTPAGETGGAVGRLGNEADTYVELNVEHKQTLDNGATTRFK
                     AMLADGQRDYNDWTGSSSNLNIRQAFAELGALPSFTGAFKDSTVWAGKRFDRDNFDIH
                     WLDSDVVFLAGTGGGIYDVKWNDTFRSNFSLYGRNFGDLDDIDNNVQNYILTMNHYAG
                     PFQLMVSGLRAKDNDDRKDANGDLIQTDAANTGVHALVGLHNDTFYGLREGTAKTALL
                     YGHGLGAEVKGIGSDGALLSEANTWRFASYGTTPLGSGWYVAPAILAQSSKDRYVKGD
                     SYEWVTFNTRLIKEVTQNFALAFEGSYQYMDLKPKGYQNHNAVNGSFYKLTFAPTLKA
                     NDINNFFSRPELRLFATWMDWSSKLDDFASNDAFGSSGFNTGGEWNFGVQMETWF"
     gene            6728..8098
                     /locus_tag="ACHJ8C_003847"
     CDS             6728..8098
                     /locus_tag="ACHJ8C_003847"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFO5553631.1"
                     /translation="MDFDKIAQSLLPLLGGKENIASAAHCATRLRLVLVDDTLADQHA
                     IGQIDGVKGCFRNSGQMQIIFGTGVVNKVYAAFIQVAGISESSKADTARLAAQKLNPF
                     QRIARLLSNIFVPIIPAIVASGLLMGLLGMVKTYGWVNADNAIYILLDMCSSAAFIIL
                     PILIGFTAAREFGGNPYLGATLGGILTHPALTNAWGVAAGFQTMNFFGFEIAMIGYQG
                     TVFPVLLAVWFMSIVEKQLRRFIPDALDLILTPFLTVVISGFIALLIIGPAGRALGDG
                     ISFVLSTLIAHAGWLAGLLFGGLYSAIVITGIHHSFHAIEAGLLGNPAIGVNFLLPIW
                     AMANVAQGGACLAVWFKTKDTKIKAITLPSAFSAMLGITEAAIFGINLRFVKPFIAAL
                     IGGAAGGAWVVSVHVYMTAVGLTAIPGMAIVQPTSLVNYIIGMVIAFAVAFSLSLLLK
                     YKTDEE"
     gene            8098..9507
                     /locus_tag="ACHJ8C_003848"
     CDS             8098..9507
                     /locus_tag="ACHJ8C_003848"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO5553632.1"
                     /translation="MSMPSQMPAILQAVMKGQSKALADAHYPCWHLAPVTGLMNDPNG
                     FCWSGGRYHLFYQWNPLACDHKYKCWGHWSSTDLLHWQHEPLALMPDKEYDRNGCYSG
                     SAVNNQGVLTLCYTGNVKFDDGSRTAWQCLATENNQGGFDKLGPVIPLPDGYTGHVRD
                     PKVWKHNSQWYMVLGAQDKEKRGKVLLYSSVDLNTWSFHGEIAGNGLNEIDNAGYMWE
                     CPDLFALDGEYILLCCPQGMVREHERYLNTYPCAWLHGQFDYETGKFTHGAFSELDAG
                     FEFYAPQTMEAPDGRRLLVGWMGVPDGEEMLQPTRKHHWQHQMTCFRELSFQKGKLFQ
                     MPIRELAQLREAEHFWQGKADHAPHVAIERLEMDIIPSGELYLNFGNALALHLNDDGI
                     QLQRRSLAGQEKLTRYWRGSVTSLKILCDSSSVEIFINNGEGVMSNRYFPHHPASLIL
                     QGESDVTLHYWSLRACMVE"
     gene            9533..10540
                     /locus_tag="ACHJ8C_003849"
     CDS             9533..10540
                     /locus_tag="ACHJ8C_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5553633.1"
                     /translation="MRKTKRVTIKDIAELAGVSKATASLVLNGRGKELRVAQETRERV
                     LSIAQQQHYQPSIHARSLRDNRSHTIGLVVPEITNYGFAVFSHELETLCREAGVQLLI
                     SCTDENPSQETMVVNNMIARQVDGLIVASSMLHDNDYQKLSEQLPVVLFDRHMNGSTL
                     PLVITDSVSPTAALVADIARSHPDEFYFLGGQPRLSPTRDRLEGFTQGLQQAGVTLQP
                     EWIIHGNYHPSSGYEMFAALCARLGRPPKALFTAACGLLEGVLRYMSQYNLLDSKIHL
                     ASFDDHYLYDSLSVRIDTIQQDNRQLAFHCFELISQLIEGETPSPLQRYLPASLQKRY
                     R"
     gene            complement(10625..11296)
                     /gene="queE"
                     /locus_tag="ACHJ8C_003850"
     CDS             complement(10625..11296)
                     /gene="queE"
                     /locus_tag="ACHJ8C_003850"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO5553634.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKEDATRLCIDTCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT         2954 a         2619 c         2758 g         3085 t
ORIGIN      
        1 accgttatga attagcgcac taaataaccg ccatcaacca ctaataaatg tccgttgaca
       61 taattcgatg ccggacttgc gaggaatacg gctgcaccca ttaaatcctg agtatcaccc
      121 caacggtttg ccggaatatg atcaagaaca cgctggttag tttctggatt actacgggtc
      181 gccagcgtaa tatcggttgc ataataacca ggagcaatac cattcacctg aatattatat
      241 tggcctaatt catcgcaata ggctttggtg aatccggcaa gagcatgttt agtggcagaa
      301 taagcaggtg accattgacc acctaagtaa gagaacaatg aacagatatt aatgatttta
      361 ccgcttttct gcgggatcat aatttttgcc gcttcgtagc ttaactcgaa tgcggcggtc
      421 aggttcacgt caatcatcgg gtcccagtcg gcacgaccaa agtccagcac cttattcagc
      481 ttacaaatac cggcattgtt aaccagaata tctacggtac cgaagcgctc acagcaggca
      541 gcgataatct tctgcggcgc gccttctgcg gtgatatcca cctgcataaa atcaacttcg
      601 acaccctgtt tttcaatcat ttcctttgtt tcgccgttat ctttgacgaa actaggaata
      661 aagacatttg cgccagcttt ggccaacgcc atggcaaatg cctggcctaa accgctattc
      721 ccgccggtaa caattgctgt tttacctttc agggagaaaa aatccattga gaacgcattg
      781 agagattcga ttgacatagt tagctccaat ttccgtaagg caaaaaaaaa gaaaggtaac
      841 ttcccccgcg agagggaagt tacctttctc attatctctg gtaactggag ataattttta
      901 gcataaggac ataactgcta atgtgatcgt aatcacagtg tgatgttcgt attgtcaaat
      961 aatttaaaaa agcagaagcg tgattactct cacacaacac ctctgctttc catgttattt
     1021 cttattgcaa ttactagaga cctgagaaaa gtaatccacc tgaaagggcg gcttactttt
     1081 ctcttttttt gtctattaca aatgggaata cgatatgcaa cacaactcat atcgccgttg
     1141 gataaccctc gcgataatta gttttagcgg cggcgttagt ttcgacctgg cttatttacg
     1201 ttatatttat caaattccca tggcaaaatt tatgggattc agcaataccg agataggttt
     1261 aataatgagt acctttggta ttgcggccat tattttctat gcccctagcg gcgttattgc
     1321 cgataaattt tcacatcgca aaatgattac ttccgcgatg atcattaccg gattactggg
     1381 tctgttaatg gcaacatatc caccgctgtg ggtaatgctc tgtattcagg tcgcctttgc
     1441 gataacgacg attttaatgc tgtggtcggt gtcgattaaa gccgcatcgt tgctgggcga
     1501 tcatagcgag caagggaaaa ttatgggctg gatggaaggg ctgcgcggcg tcggtgtaat
     1561 gtcgctggcg gtgtttacca tgtgggtctt ttctcgcttt gcaccggatg acagcgccag
     1621 cctgaaaacg gtcattatca tctacagtgt ggtttacatt ctgctgggca ttttgtgttg
     1681 gttttttgtt agcgataaca acaacctgcg caataccaat aacgaagaaa aacagtcatt
     1741 ccagcttagc gacatcctgg ccgttttgcg catcagcacc acctggtatt gcagcatggt
     1801 gatttttggt gtcttcacca tctacgccat tctgagttac tccaccaact atctgaccga
     1861 aatgtacggc atgtcgctgg tggctgcgag ctacatgggg attgtgatca acaaaatctt
     1921 ccgcgcgctg tgcggcccac ttggcggcat tatcaccacc tacagtaaag tgaaatcccc
     1981 tacccgcgtg gtccaaatcc tttccatcat cggtttgctg gcgttgacag ccctgctcgt
     2041 cacgaactct aacccgcaat cggtcgcgat ggggattggc ctgattttat tactgggatt
     2101 cacctgttac gcctcacgcg ggctgtactg ggcctgtcct ggcgaagcga gaacaccgtc
     2161 ttacattatg ggcaccacgg taggtatttg ttcggtgatt ggatttctgc cggatgtctt
     2221 cgtttacccg attatcggct actggcaaga caccctgccc gctgcagaag cctaccgcaa
     2281 catgtggcta atgggcatgg cggcgcttgg catggtgatc atctttacct ttttgctgtt
     2341 ccaaaaaatt cgtactgctg atagcgcccc cgcaatggct agcagcaagt aagccaggaa
     2401 aattacagga gaaaaatcat gtcgaaaaaa tacatcatag ggattgatgg cggaagtcag
     2461 agcacaaaag tggtgatgta cgatctggaa ggtaatgtgg tttgcgaagg caaaggctta
     2521 ttgcagccga tgcacacgcc agatgccgat accgcagaac atcctgacga cgatttatgg
     2581 gcatcattat gctttgccgg ccacgatttg atgagccagt ttgccgggaa taaagaagat
     2641 attgtcggca ttggcctggg ctcaatccgt tgctgccgtg cgttattgaa agccgatggt
     2701 acgccagccg cgccgttgat tagctggcag gatgcacgcg ttacacgccc ttacgaacac
     2761 actaatcctg acgtggccta tgtcacctct ttttcgggtt atctgacgca tcgcttaacc
     2821 ggcgagttta aagacaatat tgccaactat tttggtcagt ggccggtgga ttataagagc
     2881 tgggcatgga gcgaagatgc cgcggtaatg gagaagttta atatcccccg ccagatgctt
     2941 tttgacgtgc aaatgccggg caccattctt gggcatatca caccacaagc tgcgctggcg
     3001 acacatttcc cggctgggct gccggttgtt tgtaccacca gtgataaacc ggtagaagct
     3061 ctgggagccg ggttgctgga tgatgaaaca gcggtaattt ctcttggcac ttacatcgcg
     3121 ttgatgatga acggcaaagc actgccgaaa gatccggtgg cgtactggcc gattatgtct
     3181 tctattccgc aaacattgct gtatgaaggt tacggtatcc gcaaaggcat gtggacagtg
     3241 agctggctgc gcgacatgtt aggcgagtcg ttaattcagg atgccaaagc acaggatctt
     3301 tcaccggaag atttactcaa taaaaaagcg tcttgtgtgc cgccaggctg taatggtctg
     3361 atgacagtgc tggactggct gaccaatccg tgggaaccgt acaaacgcgg aattatgatc
     3421 ggctttgatt ccagtatgga ttacgcatgg atatatcgtt cgatactgga aagcgtggcg
     3481 ctgacgctga agaataatta cgacaatatg tgtcatgaaa tgaatcactt tgcaaagcat
     3541 gtgatcatta ctggcggcgg ttcgaacagc gatctgctta tgcagatttt tgccgacgta
     3601 ttcaaccttc ctgcacgacg taatgccatt aatggctgtg caagccttgg ggcggcgatt
     3661 aatacggcgg tagggcttgg tctgtatccg gattacgcaa cggctgtcga caaaatggtt
     3721 cgcgtgaaag atatcttcat accgattgag agcaatgcca aacgctacga cgcaatgaat
     3781 aaaggcattt tcaaagacct aaccaaacac accgatgtaa ttctgaaaaa atcgtatgaa
     3841 gtgatgcatg gtgagttagg gaatgcggat tcaatccaga gctggtcgaa tgcctaaatg
     3901 agataaacag tgccgggtag atgattaatc ccggcatatt cacgtttcca ctacaaaata
     3961 ccgggtaacc actgctgaat attgttctct cctgagtagc gtttatgctt taattgctaa
     4021 accgatttaa ttggtttagc aattagtata ttggagcctt tatgagcgca agagtatggg
     4081 tactcggtga tgcggttgtt gatttattac ccgaaagcca ggggagacta ctacagtgtc
     4141 ctggcggggc gcctgctaat gttgcagtcg gtatcgcaag gctggggggg aaaagtgcct
     4201 ttattggcaa agttggcgat gatcctttcg gtcgctttat gtatcagaca ctgagtacag
     4261 aaaatgttga tacacgttat atgtctcttg atcctcaaca acgcacctca attgtggctg
     4321 taggacttga tgagcaagga gaaagaaact ttacctttat ggtacgccca agtgccgatc
     4381 tttttttaca acctggtgac cttcctgcat ttgggccggg tgaatggctc catctttgtt
     4441 ccattgcgct cagtgcagaa ccttcccgaa gtaccgcatt tctggctatg gagaaaatac
     4501 gtcaggctgg cggaaacatc agttttgatc ccaatatccg cagcgatctc tggcagagtg
     4561 aagcgctatt aaggaaatac cttgatcgcg cactttcgct ggcgaatatc gctaaattgt
     4621 ccgaagaaga gttgctattc atcagtggcg aaagccaggt tcagcaaggc gcatattcat
     4681 tagtacaacg ttattcgttg actttattgc ttattacaca aggaaaaaat ggcgtacttg
     4741 tgtattttca ggggcagttt atccactatc ccgccaaacc tgtttctgtc gtcgatacga
     4801 ccggggcagg agatgctttt gtcgctggat tacttgccgg tctggctgat tctggaatac
     4861 caacaaatac cagacagctt gaacgaatca ttgcacaagc tcagatttgt ggtgctctgg
     4921 cgaccacggc taaaggcgcg atgaccgcct taccccgaca acacgatctc ccttcacaat
     4981 agtgatcctg ctgttgtatc tgtaacccga tttgtcgtgc ctgtcacatt tactaaatcg
     5041 gtttagcgaa aataattgct ctggctaaaa agtcagcttt actatcaata cctaaaccgg
     5101 tttagcattt tagcattttt tataattctc tgatgggacg cgacaaatta tgtataaaaa
     5161 aacaactttg gcagtgttaa ttgctttgct gaccggtgct acaacggtac atgcgcaaac
     5221 ggatattagc agtatcgaat ctcgactggc ggcgttggaa caacgtttaa aaaatgcgga
     5281 atcccgcgcc caggcggcag aagcaagggc caaaacagct gaattacagg ttcagaaact
     5341 ggctgaaaca caacaacaaa atcagctaac aactcaagaa gtagcacaga gaacagttca
     5401 gctcgaacag aaatccgcag aaaacagtgg ttttgagttt catggctatg cccgttccgg
     5461 gttactgatg aatgatgccg cttccagtag caaaagtggg ccgtatctga ctcccgcagg
     5521 tgaaactggt ggagctgttg gccgtctggg aaatgaagcc gatacctatg tcgagttaaa
     5581 tgtagaacat aaacaaacac tggataacgg tgcgaccaca cgctttaaag caatgttggc
     5641 tgacggacaa agagattaca acgactggac tggcagctcc agtaacctga atatccgaca
     5701 ggcttttgcc gaactgggcg cattaccaag ttttaccgga gcattcaaag acagtactgt
     5761 ctgggctggt aaacgctttg atcgcgacaa ttttgatatt cactggttag actccgatgt
     5821 cgtattttta gcgggaacgg gcggcggtat ctatgacgta aaatggaacg atacattccg
     5881 cagtaacttt tctctctacg gacgtaattt cggcgatctt gatgatatcg acaataacgt
     5941 tcagaactac atcctcacca tgaatcatta tgcaggcccc ttccagttga tggttagcgg
     6001 attacgggca aaagataatg atgatcgaaa agatgccaat ggtgatctca ttcaaactga
     6061 tgctgcaaat actggcgtac atgcgttagt tggtctgcac aatgacactt tctatggcct
     6121 gcgtgaaggg acggcaaaaa cagcactgct atatggccat ggcctgggtg cggaagtcaa
     6181 agggattggc tccgatggcg ctctgctgtc tgaggcgaat acctggcgct tcgcatctta
     6241 cggcacaaca cctctgggaa gcggttggta tgttgcgcca gcaattctcg cacaaagcag
     6301 taaagatcgt tacgtcaaag gcgatagcta cgaatgggtg accttcaata cacgtctgat
     6361 caaagaggta acacagaatt ttgctctggc ctttgagggt agctatcaat atatggatct
     6421 gaagccaaag gggtatcaaa accacaacgc cgtaaacggc agcttctata aactcacctt
     6481 tgctccaact ctaaaagcta acgatatcaa taatttcttt agccgtccgg agcttcgcct
     6541 gtttgccacc tggatggact ggagcagcaa acttgatgat tttgccagca atgacgcttt
     6601 cggcagcagt ggtttcaata ctggcggaga gtggaatttt ggtgtccaaa tggaaacctg
     6661 gttttaatac cagacagcct cacggatgtg aggcttttac tgaaataaca atgaattaga
     6721 gggttatatg gatttcgata aaatcgccca atcgttgctt cctttattag gtggtaagga
     6781 gaacatcgcc agtgcagcac attgcgccac tcgcctacgg ctggtactgg ttgacgacac
     6841 acttgccgat caacatgcca ttggccagat tgatggagtg aaaggttgtt ttcgtaattc
     6901 agggcaaatg caaatcatct ttggcaccgg cgtggttaat aaagtctatg ctgcctttat
     6961 tcaggttgca ggtattagcg aatccagtaa agcagatacc gctcgactcg ccgctcaaaa
     7021 actcaatcct tttcagcgaa tagcacggct actttctaat atttttgtcc ccatcattcc
     7081 agccattgtt gcttctggtt tattaatggg gctcctggga atggtgaaaa catatggctg
     7141 ggtgaatgcc gataatgcga tttatatcct gctggatatg tgcagttcag ccgcatttat
     7201 tattctcccc atccttattg gctttactgc tgctcgtgaa tttggtggaa atccttatct
     7261 tggcgcgaca ttaggaggga ttctgactca cccggcactc acaaatgcct ggggcgttgc
     7321 tgcgggattt cagacaatga acttcttcgg ttttgaaatc gccatgattg gctatcaggg
     7381 aacggttttc cccgtgcttc tggcagtatg gtttatgagc atagtggaaa aacagttacg
     7441 caggtttatc cctgatgctc tggatctcat tctgacgcca tttctgactg tcgtcatttc
     7501 tggctttatc gctcttttga ttattggtcc ggcaggacga gcgttaggtg acggtatctc
     7561 tttcgttctc agcacgctga ttgcacacgc aggctggtta gcagggttgt tattcggcgg
     7621 gctatattca gcgattgtta ttacgggtat tcatcatagc ttccacgcaa ttgaagcagg
     7681 acttttaggt aaccctgcaa tcggtgttaa tttcctgctg cctatttggg caatggcaaa
     7741 cgttgcgcaa ggcggtgcat gtctggcggt atggtttaaa accaaagata caaaaattaa
     7801 agcaatcacc ctaccctcgg ccttttccgc aatgttaggg attactgaag ccgctatttt
     7861 tggtatcaac cttcgttttg ttaaaccttt tattgcggcc ttaattggtg gcgctgccgg
     7921 cggtgcctgg gttgtctctg ttcacgttta tatgactgcc gttggtctga ccgcaatacc
     7981 cggcatggca atcgttcagc caacatcgtt ggttaattat attattggaa tggtgattgc
     8041 tttcgctgtc gcttttagcc tctctttatt actcaaatac aaaacggacg aggagtaatg
     8101 tcgatgcctt cacaaatgcc cgcgatttta caggcagtga tgaaaggtca gtccaaagca
     8161 ctggcagatg cccactatcc ttgttggcat ctcgctccag tcaccggact aatgaacgat
     8221 ccaaatggct tttgttggtc tggaggccgc tatcatctgt tttatcaatg gaatccactg
     8281 gcttgtgatc ataagtacaa atgttggggg cactggagtt cgacggattt attgcactgg
     8341 cagcatgaac cattagcgct aatgcccgat aaagagtacg accgtaatgg ttgttactca
     8401 ggaagcgcgg ttaataatca gggcgttctc acactttgct ataccggtaa tgttaagttt
     8461 gatgatggca gcagaacggc atggcaatgc ctggccaccg aaaataacca gggcgggttt
     8521 gataaactag gccctgtcat accgttacct gatggttaca ccggacatgt tcgtgatcca
     8581 aaagtctgga agcacaacag ccaatggtac atggtgcttg gcgcgcaaga taaagagaaa
     8641 cgggggaaag tgctgctgta ttcctctgtc gatctgaata cctggtcttt tcatggagaa
     8701 attgctggta acggtctgaa tgaaatagat aacgcaggat acatgtggga atgtcctgat
     8761 cttttcgccc ttgatggtga atacattctc ctctgttgcc cccagggaat ggtgcgcgaa
     8821 catgagcgct atctgaatac ttatccctgc gcctggctac atggacagtt tgattacgag
     8881 acaggcaaat ttacgcatgg cgctttctca gagctggatg cgggatttga attctatgca
     8941 ccacagacga tggaagcccc ggacgggcga cgattgctgg tcggctggat gggggttcct
     9001 gatggcgaag agatgttgca gccaaccaga aagcatcatt ggcagcatca aatgacctgt
     9061 tttcgcgaac tctcattcca gaagggaaaa ttgtttcaga tgccaatcag ggaactcgcg
     9121 caactgcgtg aagctgaaca cttctggcaa ggaaaagcgg atcatgcgcc gcatgtcgca
     9181 attgaacgcc tggaaatgga catcattccg tcgggtgagc tgtatctgaa ttttggcaat
     9241 gctctggcac tgcatcttaa tgacgatggt attcaactac agaggagaag ccttgcaggg
     9301 caagagaaac taactcgtta ctggcgagga agcgtaacgt cactgaagat tctctgtgac
     9361 agttccagcg tagagatatt catcaataat ggtgaaggtg taatgagtaa tcgttatttt
     9421 ccccatcatc cagcttcgct gatattacag ggtgagtctg acgtcacgtt acactactgg
     9481 tcgctacgcg cctgcatggt agaatgaggg ttttgaatta cggatcctcg tcgtgagaaa
     9541 aaccaaacgc gttactatta aagacattgc tgaactggcg ggagtgtcga aagcaaccgc
     9601 cagtctggtt ctcaatggtc gtggcaaaga gttacgtgtt gcgcaggaaa cgcgtgagcg
     9661 tgtgctgagt attgcccaac agcagcatta tcagcccagc attcatgccc gctcattacg
     9721 ggacaaccgc agtcatacca ttggtcttgt cgttcctgaa atcactaact acggatttgc
     9781 agtattttcc catgaactgg aaacattgtg tcgggaagcg ggcgtccaac tcctcatatc
     9841 ctgtactgat gaaaatcctt cacaggaaac aatggtggtg aacaatatga ttgcccgcca
     9901 ggttgatggg ttaatcgtcg catccagtat gctgcatgat aatgactatc aaaaacttag
     9961 cgagcaatta cctgttgtgt tgttcgatcg ccacatgaac ggcagtacat tgccattggt
    10021 cattactgac tctgtttctc ccaccgccgc tctggtggca gatattgcaa ggagtcaccc
    10081 ggatgaattc tattttctgg gcggacaacc ccgtttgtca ccgactcgcg atcgactgga
    10141 aggatttaca caaggcttgc agcaagccgg agtaacgcta caacctgaat ggattattca
    10201 cggtaattat catccgagct caggatatga aatgtttgcc gcgctatgcg ctcgtttagg
    10261 acgtccccct aaagccttat tcactgccgc ttgtggatta ctggaaggcg ttctgcgcta
    10321 tatgagccaa tataatttac tcgacagtaa aatacacctg gcgagttttg atgatcatta
    10381 cttgtatgat tcactttcgg taagaatcga cacaatacaa caggataatc gccaacttgc
    10441 ttttcactgc tttgaactta tttcgcaact gattgaaggt gaaacgccat ccccattaca
    10501 gcgttatctt cctgccagtc tacaaaaacg ctaccgataa tcccgtcact atcttgcatc
    10561 gcccctgtaa aacggggcga ttattttcat cctgaacgtt aaatattaat gatgaataat
    10621 caaatcaggc aatattcaaa tatttatgtg tctgcatcga caaacgccag ttgcgcgcaa
    10681 tgcaggtatc gatacataaa cgcgtagcat cttccttctg gctaattggc tgaagtgcaa
    10741 tgactcgcgg tttatcatcg gtcagcgtcg ccagcaattc gtccagtgct tcgatatcac
    10801 gtacgcgtcc aactgggtgt ttgatttcgt tagctcgctc cagggcttgc gacaatactt
    10861 catagccgcc gcgcatgttc agctttggcg atacggtaac ccaggtattc ggtgtgcagc
    10921 gtacctcatg agtaccactg gtttcgatct ggcagctaaa accgttcttt tcgagcaggt
    10981 cagtcagtgg cagcaaatca tgaatgcaag gctcaccacc cgtaatcacc acgtgacgcg
    11041 cggtgtatcc ctgacggcca atgacagcca gcaaatcttc actgctcgcc gccccccact
    11101 tatcactctc tttggtcttc gccagaatgc tgaaaaggga gacttcccga tcctcaagct
    11161 tttcccaggt gtgtttggtg tcgcaccagg cacagccaac cggacatccc tgtaaacgaa
    11221 taaaaatggc ggggacgccg gtaaagtaac cctcaccttg cagggtctgg aacatctcgt
    11281 taatcgggta ctgcatagca ttctctgtga agtggataat tgttaattat tgcagatcct
    11341 gccacaacaa tcatgtctta ttaacattct gttacaggca ggtttgatgc aaaaattggc
    11401 tcagatcatt aagaat
//
LOCUS       DAZFJL010000139        11401 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFJL010000139 DAZFJL010000000
VERSION     DAZFJL010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.141"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(94..867)
                     /gene="uxuR"
                     /locus_tag="ACHJ8C_003851"
     CDS             complement(94..867)
                     /gene="uxuR"
                     /locus_tag="ACHJ8C_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO5553635.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNADSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1083..2543)
                     /gene="uxuB"
                     /locus_tag="ACHJ8C_003852"
     CDS             complement(1083..2543)
                     /gene="uxuB"
                     /locus_tag="ACHJ8C_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO5553636.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2624..3808)
                     /gene="uxuA"
                     /locus_tag="ACHJ8C_003853"
     CDS             complement(2624..3808)
                     /gene="uxuA"
                     /locus_tag="ACHJ8C_003853"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO5553637.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4257..4364
                     /locus_tag="ACHJ8C_003854"
                     /pseudo
     CDS             4257..4364
                     /locus_tag="ACHJ8C_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4537..5439)
                     /gene="fimH"
                     /locus_tag="ACHJ8C_003855"
     CDS             complement(4537..5439)
                     /gene="fimH"
                     /locus_tag="ACHJ8C_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO5553638.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSSFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(5459..5902)
                     /locus_tag="ACHJ8C_003856"
     CDS             complement(5459..5902)
                     /locus_tag="ACHJ8C_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5553639.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGATDSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(5975..6505)
                     /gene="fimF"
                     /locus_tag="ACHJ8C_003857"
     CDS             complement(5975..6505)
                     /gene="fimF"
                     /locus_tag="ACHJ8C_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO5553640.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNEIPLNAPSSAISWTPLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(6515..9151)
                     /gene="fimD"
                     /locus_tag="ACHJ8C_003858"
     CDS             complement(6515..9151)
                     /gene="fimD"
                     /locus_tag="ACHJ8C_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO5553641.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAQLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(9217..9942)
                     /gene="fimC"
                     /locus_tag="ACHJ8C_003859"
     CDS             complement(9217..9942)
                     /gene="fimC"
                     /locus_tag="ACHJ8C_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO5553642.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMVVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESAVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(9979..10518)
                     /gene="fimI"
                     /locus_tag="ACHJ8C_003860"
     CDS             complement(9979..10518)
                     /gene="fimI"
                     /locus_tag="ACHJ8C_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5553643.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAEACR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSSPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPVNWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(10583..11131)
                     /gene="fimA"
                     /locus_tag="ACHJ8C_003861"
     CDS             complement(10583..11131)
                     /gene="fimA"
                     /locus_tag="ACHJ8C_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO5553644.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AAHTNVLALQSSAAGSATNVGVQILDRTGVALTLDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT         2759 a         3015 c         2741 g         2886 t
ORIGIN      
        1 aatttacgca taatatattc tgcagaacat aattaactat tcagggaaca tacatagtgc
       61 aaagcggtta aactttgcac cagaaatgag ggatcaggcg tcgactttat ccagcggcca
      121 tgaatcaaac agatagccat cgaaatagat gtcatcaaca ttagagaact ccagcaagcg
      181 ctgcttcaca ttctccagat gctgccacat tgccagcttc gccgctcggg catctttttt
      241 gattaacgcg gcgaggatct gtttgtgatc gcccaaccac tctttgcgat acaggctgtc
      301 atccaggtgg ctgtgcaact ggatccacat tggattgttt tcccgccact gccaggactg
      361 acggaataac tccaccagca tgctgttatg cgttgcttcg gcaatagcga gatggaactg
      421 catgtcaccg ctttcgctgc tgcccggcgc gctggaagcc agttcacgct cttccagttg
      481 caatgcctgg cgcattttga cgatatcttc gcgggtagcc tgcagggcgg caaactcggc
      541 gatgttactt tccagtaact gccgcgcctg taacagctca aaaggaccgg catcgttgca
      601 gacgttggca tccggactgt ctgcgttgtg gctgcctgag ctatcaagaa catagatacc
      661 tgcaccccga cgcacttcca ccagcccttt gatctccagc atgatcagcg cttcacgtac
      721 caccgtccgc gtgacatcaa gcatttctgc aatttcacgc tccggtggca gccgttcgcc
      781 aggattgtac ggcgtcttta tgatcagatc gcggatcatc gccccgactt cctggtaagg
      841 tctttgcgca gaggtggcag atttcatggt gttgtgtccg ttaggtgcgg ttaatcaaat
      901 ttttttccgg atgcggcgtg aacgccttat ccgacctaca acgggcaatt ttacggcacc
      961 cgttaagctc aaactatacc attagcactg gatgttggca cgtacaggtg ggcaaaaaaa
     1021 cgaaaactgg tcaaccaaat attagtttga ttgaccagta agtctgtagg gtaatcagtt
     1081 agttacagcg cagccacaca ctcgcgcgca ccgcgctcac acagctgctg atatgccgcc
     1141 gtcacagcgc caacaaagtc ggcgttctgc ggcagatcat cggcaaagat accgctcagg
     1201 cccagcagtg ctttcacgcg gtctgcaccc tgatactgcg cgttgatctt ctggaactcc
     1261 gccagcatcg ggtcaaccac gtcaatggca ttaccctgct catccacgcc ctgggtgtag
     1321 cgcatccagc ccgccacgcc cagcgccagg tgacgccagc tgccgccgtt ttgcaggtgc
     1381 agacgcacag ggtccagcag acgctgcggt aacttctggc tgccgtccat cgcaatctgc
     1441 caggtgcggt gacgcagaga cgggttgctg aaacgctcga tcagcagcgt cgcataggcg
     1501 ttcaggtccg taccttccgg catcgacagc gttggcgctt gttcctgcat catcagggca
     1561 aaggccgctt tgcgataagc cgggttagtc atggtgtcgg caatagtttc atagccgccg
     1621 aggtaaccga ggtacgccag gaaagagtgg ctaccgttca gcatacgcag cttcatcatt
     1681 tcgaacggta caacatccgc aacaaactgc gcgccgactt tatcccagtc cggacgacca
     1741 ttaacgaagt tatcttcaat tacccactga cggaacggtt cgcaggcaat ggcgcacggg
     1801 tcgtaaacac ccagctggtc agcaatttcc tgtaaggttt ctggcgtcgc cgccggaacg
     1861 atgcggtcaa ccatggtgca cgggaaggtg acgttctctt caatccatgc cgccagttgc
     1921 gggtcacgcg cctgagccag cccaagaacg gcaaccttcg ccacatgacc gttttcacgc
     1981 acgttatcgc aggacatcac cgtaaacgct ttcagccctt tttcacgacg cagacgcagg
     2041 gcttctacga tgtaaccaat cgcagacttc ggcgcagtcg ggttttcgag atcgtgcttg
     2101 atcagcgggt tattgagatc cagctgaccg cttgccgcat cagcgcagta gcctttttcc
     2161 gtgaccgtca gagagacaat cgccgtctgc ggacgcgcca tcgcgttgag aataccttcg
     2221 caaccgtcga tttccggatg cagcgcttct ttcatcgaac cgataatttt cagctcggtg
     2281 ctttccgcgc ctttttccgc cacggtgtac agcagttgct gttttttcag gttttcgatc
     2341 agcacgcggt cgttgcctgg catcaggtta acttcgcaga taccccagtc gctgtcggta
     2401 ctttccagca gatgatgggt atacagcgcc tggtgcgcgc ggtgaaacgc cccgcaaccg
     2461 agatgcacaa tgcgtgattc cagacgagaa tgatcccatg acgggcgggc aaccggcaga
     2521 ttgctgtcaa caatagtagt catcgtaaac tccagaatcc gtcttcagag agtgacggca
     2581 aggaagaagg aaccggcacg gcgcgcagcc atgccggtgg gtattaacgg ctaaagaaag
     2641 cgcgctggat cgccagttcg acaccgcgaa cttcggccag gcctttcaga cgaccaattg
     2701 cggagtaacc aggattggtt ttcttcttca ggtcgtccag catctgatga ccgtggtccg
     2761 gacgcatcgg gatcaggtct tctttgcctt ccgctttacg acggtgttct tcttcaacaa
     2821 tcgctttcac cacttcgtac atatcaacgt caccgttcag gtgcgccgct tcgtggaagg
     2881 ttttcgggtt atcttcacgc atggtggagc gcagatgggt gaagtaaata cgcgggccga
     2941 actgcttgat catatcaacc agatcgttgt cagcacgcac gccgtaggaa ccggtgcaca
     3001 tggtgaaacc gttcgccatg ctgtttacgg tatcaaccat ccactgcata tcttcaatgg
     3061 tggaaacaat gcgcggcagg ccgaggatcg ggcgcggcgg atcgtccggg tgaaccgcca
     3121 tacggacgcc aacttcttcg gcaaccggaa taatggcttt caggaagaca gcaaagtttt
     3181 cgcgcagttt ggctttgtcg atatctttgt acagctccag gtgcttacgg aactggtcga
     3241 gggtataacc ttcttccgcg cccggcagac cggcgatgat gttacgggtc agacgcgctt
     3301 tgtcttcgtc gctcatagtg gcgaagcgtt cagcggcctg agcaatttct tcttcggtgt
     3361 aatccgcttc cgcgcctgga cgcttcagga tatgcatttc gaatgcagcg aattcgatct
     3421 ggtcgaagcg cagagctttg gagccgtctg gcagcacgta ttcgaggtca gtacgggtcc
     3481 agtcgagcac cggcatgaag ttgtagcaca cggtgcgaat gccgcactgc gccaggttgc
     3541 gcagggtctg ctgatagtta gcaatccact gctcatagtt gccagtgtgg gttttgatat
     3601 cttcgtgaat tggtacgctt tctacgacag accataccag gcctgcgtct tcaacgatcg
     3661 ccttgcgttt gaggatctct tcaacggacc atacttcgcc gttcgggata tggtgcagcg
     3721 cggtaaccac gccagttgcg cccgcctgac ggacatcagc taaagaaacc ggatcgtttg
     3781 ggccgtacca gcgccaggtc tgttccatga tttttcctct ttcacatagt gggttcaaca
     3841 gcgaagagac gcggaacccc ggagttgttt aggtatccgg atgaacaacc cggccacagc
     3901 cgtagcgtcg catcggatga gatggcgtat aagttctacc ttttggtaag acatgtcaac
     3961 ccgaattcta aaattggtta accacatcac aagaatttca ctctcctggt tgttgctgca
     4021 aattgcagca aagggtaatc gatggttatt gatcagagga tgtgacattc atcgcaacaa
     4081 tggttgacca atttacataa catgtcgccc aaaataacaa cggttcaacc aggtaaggta
     4141 aaacgttatg catgtgctta acattctctg ggtggtattc ggcattggcc tgatgctggt
     4201 actgacaatg accgcaggtt cagacacatt ccctacatta gcaatgcctc ttagattact
     4261 ttgacctgtc agtaaaagac acgttgaaaa cctggggtct gctggagctg gtcaactccg
     4321 tggttgggct gattatggtg ttgattatta gcatggtagc gtaaaagaac accagagcct
     4381 gccaatggca ggctctgttc ggattgtcgg caaagtgcgc tttgtttatg ccagatgcga
     4441 cgctgacgcg tcttatctgg cctacaaagg gctaacgtgc aggttttagc ttcaggtaat
     4501 attgcgtacc tgcattagca atgccctgtg atttctttat tgataaacaa aagtcacgcc
     4561 aataatcgat tgcacattcc ctgcagtcac ctgccctccg gtacgtgcgt aatttgccgt
     4621 taatcccaga cttaccgccg aagtccctac tgctcctaac gataccgtgt tattcgctgg
     4681 aataatcgta ccgttgcgcg tcaactgtac gccgacgccc tgtgcaggtg aaaacgacgc
     4741 ggtattggtg aaaatcgagt tgcccgcatc tgcggttgtg ccggagaggt aataccccag
     4801 gttttggctt ttcgcacaat aaacggtaag aggaatcggc actgaaccag ggtagtccgg
     4861 cagagtaacg gtgacatcac gagcagaaac atcgcagccg ccagtgggca ccaccacatc
     4921 attattggcg taaatattcc acacaaactg gaaatcatcg ctgttatagt tgttggtctg
     4981 tcgcaaaata agcacggcaa ttaatgagcc agctttaatc gccactcccc ccgcactgct
     5041 cacaggcgtc aaataaagcg ccaccggcca cggcttatcc gttctcgaat tataaacaac
     5101 ccgcggcgtt tcgctggtag tagggaaagg atagctactg ccactatatt ttacggtccc
     5161 ggaaaaacta gataacacgc cgccataagc cgagcctcgt tgcagtgtga cataatctgt
     5221 aatggtttcc gggtaatcgt tatggcaaaa gatttgcgtc gaaagatcca cgaccaggtt
     5281 ttgccccaca ttcacggcag gcgcaaggtt tacataaaca ttggcgctgc caccgccaat
     5341 agggattgcg gtaccattgg cggttttaca ggcgaatgac caggcattta ccgaccagcc
     5401 catcagcagt acagcaaaca gggtaataac tcgtttcatt acaatcatct ctttgggttc
     5461 agctgtaggt ataggtgatg ctaatcactg cctgaatggt tccctgagtg gctccgccat
     5521 ttaccgtcaa tgctctgacc tgtaacggga agtgcgctga ttgtgaggaa tcatccacct
     5581 gaactgtttt ggttgcgcca gtattcaatg tgttgccact gtcatcctgt agctctaact
     5641 ggatgttttg cgcggtcccc tggtttttat aatatccggt actgtcggtt gccccgctga
     5701 agctggcagt gaccctcgac gttcccaccg gacaattagt caactcaagc gcaacatcat
     5761 gccaggccga tgccgccccg gcagacatca gactgaaaga ataaagatcg ccgagatcta
     5821 ccgtggcatt ggtggtggaa accgtacacg gtttggcgac gaccttaccg ttcaccgtga
     5881 tggtgacatc ggctgcctgt atcgtcgcac ttgcgaacgc caacattgcc gccaataaat
     5941 acccacgttt gcaccatttc atgagcatct ccagttactg atattcaaga gtgaaggtag
     6001 ccgtggcatt gatatgcccc gcagtgacag gcacctgtgt cgccattagc cgggcgtaaa
     6061 aattcaacgt atttggttta cccggcgtca ggggcgtcca cgaaatcgcg gacgatgggg
     6121 cattaagggg tatctcattt tgctgctcat tcagaagctg tattcccagt cccgcagccg
     6181 ctgacaccgt attttcaagt gcaagcaggt tggcattgtg gctatctgca acgccggtaa
     6241 acccaacctt tacggcagaa acagcgttac cacagggtga cagcaaaata cgaaatggaa
     6301 cgacaggagt cgtcgcgcca atgttgttaa attgcttcgc cgcgttttcc atcagatcaa
     6361 cagtaaaatt ggttgattca gcggccacac tacagccatt atctctgaca tagccgcgga
     6421 tagtaatcgt gctatccgca gccaaagcgt gacttaccgc cagccacaaa aaagcgcaaa
     6481 gaagataaaa aggtttgttt ctcatcacgc ctccttaacg acattcagct gatagctggg
     6541 ttaataactg ctgctgactc tctggtggca gttgataatt ggcgacacag tgagcatttt
     6601 cctcttctcc ccatttcacc tgaacttttc ccgctaaagg cattccgctg aggtaaacct
     6661 gaccattatc cgcaacaatg ccgctactct ggctactctc tgatgtcacc atcgccccaa
     6721 acggcagcgg cttattattg tgggttagcg tcatgagcag ttttatccca acgcgcgctt
     6781 taaactctgc tcgcacgatc gccccacggg tgggaacaac gttagcgacc gcattatcta
     6841 aatcgacgtt atcagccagg gtattggtat ccagcgccac tctgttttcc cgatattcag
     6901 tggcataagg cagcacggca taaccccgcc agtcggtacg cactcctgtc tggttttcga
     6961 cttttgcatc ttttgcgcca ggcgctttaa caagcaccac cgtatcgttt aacggctgcc
     7021 ccagcgttac gccattggca tgagccagta ccccaccgct gactccgtaa tagagctgct
     7081 taatatcatc gctatggctg taaccgatat tggcattgcc gtaaccaccg cgataattca
     7141 gcgtggcgta gcctgtactt ccgctatttc catcgcctcc cccggcataa ccggtttgca
     7201 cgctatagct gaggttgttg tcttccagca acgtaccgta tacaccggcc agattggtca
     7261 tccgaccgtt gagatcgtgt gacatgctgt agctggcact ggcatgtcgc cactgagatt
     7321 tactgtcaga acgcagccag tggctgaaag gaatattgac gttaagagct aacatctgat
     7381 cccgtccttt ttgccaggcg tttttcgtca ggctatagct gagcgtccag ttaatatctt
     7441 cgaacgcagt atttaatcca gcctggaatt gctcatcgac attactcgtt ccccaataag
     7501 tttgatggct accactcaaa tacagtgttg atgtgcgccc gagttgctga gtaacggtga
     7561 gttgtaattt cccgcgtttg ttataagcga gattgtaata gtcggtgaat ttcggcttaa
     7621 cctgaataac tccatcctgt gtttcgatgt tgtagccatt cattcgactg tatgttgtat
     7681 cagcgaaatt aaaataccca ctggtcgaat aacggtaacc cactaactga atattcgtgc
     7741 ctgactcatt gagcgatttg ttataaagaa aacgcaccga ttgtccgtca tgctgactgt
     7801 catcgggaag tgtggaatta gcctgcgtca tatccacaga cagagcgccc agtgccccca
     7861 tatttttccc gataccgaaa ttaaacgcac gataacgatc cgccagttgc gttccaccat
     7921 atattgtcca gccagccgga aggccgtgga gtaatgtact ctggaaaaag cggggttttt
     7981 cctgttgcgc atttccacta cggtattctc ctgccgtaat ggaataacgt gtatgccctt
     8041 cacgctgcaa aagcgggact gacgaatagg gtacggtaaa aatctgcgta ctgccgtcag
     8101 cctctttaat cgttacctgc aagtcaccac tattacctgc ggcatagata tcgttgatgg
     8161 taaaaggccc cggtggcacc gtactattat aaatgtcata cccattttgt ttaatagtga
     8221 cctgtgcagt accacgagca ataccgtgga tcaccggggc aaatcctctt tggctatcgg
     8281 gtaacatatt gtcatctgag gccaattgtg cgccgcgaaa gttaatacca tcgaaaatat
     8341 cgccctgagt ataaccatca cccagcgtca gccgggaacg taacggaatt atgtctcgct
     8401 caagccaggt attgatatgc tgccatttat ttttgctacc tgatgaactg tcgctactgt
     8461 tataactcca ggtggtattg tcgcgtaaac gccacgcacc aatatttaac ccactctgta
     8521 ggtttaaata tgcataatgg ctgttacccc caatccgatt ctgtacacta tttccgctga
     8581 aattataatt gagcaatccg gcattaatac cgggatccca taactcagga ggaatataac
     8641 cacgcgcgcg attactcata aatgcctgag ggatcgtcag gttgagtcgc tgctgaccca
     8701 catccagttg cgcagtagcg tcatggatca ttgaggttaa tggcacgcag gcatcatccg
     8761 ccagcagatt cataccggag acagaagccg tattcagccc catactggcg agttgcgcgc
     8821 gtgtcaggca gggaacaatc ccttgttcgc tgtcgcccgt attaaatgtg acatcacgcg
     8881 ttgccatata accattattc aaatagatat caacacgata cgtccctggc ggtaattctt
     8941 gcccattctc aaaacgcgat aaatcagcca cagcctgggg atcatccgct aaaaaacgcg
     9001 ggttaaaata gagttcggca gatgacaaag gtgcctgtgc ggcaaaagca caggcaacaa
     9061 agagccggac aaaaaaacca gccaaacgat gcttacgaat atgcaagcat tgtgtgtttc
     9121 gctggtaaag tcttaaattc agatatgaca ttattcctcc ggtttaaatc acccggcagt
     9181 caattctttt tattcaggcg aaaaaatccc cctgcgttat tccattacgc ccgtcatttt
     9241 gggggtaagt gcgccataat catttattgt tcggtaagta atattgcttc ctgcatcaga
     9301 aggcaattta accgcgcttt cgcccattgg aggcactaat gcattttcaa gaacccgagt
     9361 tccggcattc aactctgtta ccgtcaggta atagggtgtc gggttgatca gcgtcagaga
     9421 attcgcgcta cgacgaaatc ttaatttttc tgcggcctga tcgggtggca acgctaattt
     9481 agccggacga tagtacagtt taatgcggct gataattgcg agctgtagcg tattctcagt
     9541 caattttgat ttatccattg acggaatcgc tttaacattc atccagaata aactttcccg
     9601 atcctgtggc aattggttat ttgttgcatc aagaatacgc aaggtattct ctttttttcc
     9661 cttcatcgca aacagaggtg gcgtcacgat aaaacggcca tcctttacac catcggcatt
     9721 ttctacccat gattgaatta aataggtgct attttcatca ttatttgtca cggcaagttg
     9781 cacttgtttt tgccctgccg gataaattac gcgagtcgca cctaaggcca ctcccgcttc
     9841 agcgcgtccg gcaaccacca ttgccatgaa catcaggata cctgccagca agcagaatgt
     9901 tatttcctgc gatttcctta cattgacgtt tttattactc actgtcctgt tcctgttatc
     9961 acattatctg ctgaacaatt actgataggt taaagagaac caggcctggg cattggcgat
    10021 gccgccagta acccgacgcc cggtagcacg atatttggcg atgaaatgta gcgaagtaga
    10081 gcctgagtaa agccgtttcc agtttactgg aggacgatta atcggtacga ggtttccttc
    10141 atcatcaaac aacgctacgc caatattggt ggctatccct ggcccctctc ccacggaaag
    10201 cacatccgga tttttaccat ccgcgacacc gtgaaacgcc acacccacac gttcactcac
    10261 caccgtgcta cattcccgta aatgaataac aaaaggcacc ggtgagctat cttcccctac
    10321 cgcatgaaac cggttactgc tgatttgccc catattgacc gtcatttgtt tatcaccggc
    10381 ttcaatccgg caagcttccg caataatgac gccctgaaat tgcatatttc cgccgggcag
    10441 cgtggtattc catttatttc cggccagagc aaacattggc agcaacgacg ctaacagaaa
    10501 taatctcttc cttttcatcg ttatttttat cgcacaaggg tgggcatccc tgcccgtaat
    10561 gacgtccctg aacctgggta ggttattgat actgaacctt gaaggtcgca tccgcattag
    10621 cagcacccgg ggttgcagca ccggttgcaa aataacgcgc ctggaacgga atggtgttag
    10681 taccgttatt cagggttgtt tctgaactaa atgtcgcacc gtccagcgtc agcgcaacac
    10741 ctgttctgtc cagaatctgc acaccaacat ttgttgcgct acccgcagcc gaactctgca
    10801 gagccagaac gttggtatga gccgcatcaa tcgccgtacc taaaaaggca acagctgctt
    10861 tagatgcaac attggtatcg caatcattca gctgaatgtt aaaaccgaca gcagaactgg
    10921 ttgctccttc ctgcgccagc gatgcggtac gaacctgtcc taactgaacg gtttgatcaa
    10981 cagagcctgc atcaactgcg caagcggcgt taacaacttc ccctttaaag tgaacggtcc
    11041 caccattaac cgtcgtggca gcggccagag ccgctgtgga actgagggac agagccgaca
    11101 gaacaacgat tgccagagtt ttaattttca tgctgctttc ctttaaaaaa actatttcta
    11161 aatcgacatg ggcagtcgtt ctgtacactt tgttttgtca acgagtttga ctgccaacac
    11221 tgcacagttt tcccccaaaa gatgaaacat ttggggccat tttgactcat agaggaaagc
    11281 atcgcgcaca aactttttca gtttatttgt tggcttaatg ttctatagtt atctttattt
    11341 gcagtttttt atattgcatg aggtggtttt tggagagaag aatgaggaag ttgcgtcgag
    11401 c
//
LOCUS       DAZFJL010000140        11383 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFJL010000140 DAZFJL010000000
VERSION     DAZFJL010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.142"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            236..535
                     /gene="yoaC"
                     /locus_tag="ACHJ8C_003862"
     CDS             236..535
                     /gene="yoaC"
                     /locus_tag="ACHJ8C_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO5553645.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(655..834)
                     /gene="yoaH"
                     /locus_tag="ACHJ8C_003863"
     CDS             complement(655..834)
                     /gene="yoaH"
                     /locus_tag="ACHJ8C_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO5553646.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            908..2269
                     /gene="pabB"
                     /locus_tag="ACHJ8C_003864"
     CDS             908..2269
                     /gene="pabB"
                     /locus_tag="ACHJ8C_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO5553647.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAQQDIVLPDMAVGIYDWALVVDHQRQTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGVILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSPKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2273..2851
                     /gene="nudL"
                     /locus_tag="ACHJ8C_003865"
     CDS             2273..2851
                     /gene="nudL"
                     /locus_tag="ACHJ8C_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO5553648.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3035..4399
                     /gene="sdaA"
                     /locus_tag="ACHJ8C_003866"
     CDS             3035..4399
                     /gene="sdaA"
                     /locus_tag="ACHJ8C_003866"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO5553649.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGFSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4530..6128
                     /gene="pdeD"
                     /locus_tag="ACHJ8C_003867"
     CDS             4530..6128
                     /gene="pdeD"
                     /locus_tag="ACHJ8C_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO5553650.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDSRHLLYGNGLVDSLPQPENNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRSHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6132..7688)
                     /gene="yoaE"
                     /locus_tag="ACHJ8C_003868"
     CDS             complement(6132..7688)
                     /gene="yoaE"
                     /locus_tag="ACHJ8C_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO5553651.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7676..7834)
                     /gene="yoaL"
                     /locus_tag="ACHJ8C_003869"
     CDS             complement(7676..7834)
                     /gene="yoaL"
                     /locus_tag="ACHJ8C_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO5553652.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8152..9123
                     /gene="manX"
                     /locus_tag="ACHJ8C_003870"
     CDS             8152..9123
                     /gene="manX"
                     /locus_tag="ACHJ8C_003870"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO5553653.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPIEKAAPAPAAAAPKVAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9186..9986
                     /gene="manY"
                     /locus_tag="ACHJ8C_003871"
     CDS             9186..9986
                     /gene="manY"
                     /locus_tag="ACHJ8C_003871"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO5553654.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9999..10850
                     /gene="manZ"
                     /locus_tag="ACHJ8C_003872"
     CDS             9999..10850
                     /gene="manZ"
                     /locus_tag="ACHJ8C_003872"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO5553655.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10905..11363
                     /gene="yobD"
                     /locus_tag="ACHJ8C_003873"
     CDS             10905..11363
                     /gene="yobD"
                     /locus_tag="ACHJ8C_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO5553656.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKVYKLLVSTQ"
BASE COUNT         2794 a         2888 c         2854 g         2847 t
ORIGIN      
        1 cttcgattag catctctggt tttctttctt agctgaacaa tttggttata gcgtgcggac
       61 tgaccgttaa ttttctccat cttactggat aacacaccat gagctgctac ctttaaaaca
      121 cctgaatgtc aacaggttaa ctcgtgccat atcgttataa tccctttgat attagatgca
      181 aattaaggtc atatatagcc ttatcgataa catggttaat tttaaggata agaatatgcc
      241 tgctgtaata gataaagccc tggatttcat tggtgccatg gatgtatcag cgccaacacc
      301 aagttcgatg aatgaaagca cggcgaaggg aatctttaaa tatttaaaag aactgggagt
      361 acccgcgagt gccgctgata ttacggcgcg agctgaccag gaaggctgga acccggggtt
      421 cacggaaaaa atggttggat gggcaaaaaa aatggagaca ggtgaacgct ctgtgattaa
      481 aaatcctgaa tacttttcaa catatatgca ggaagaacta aaagcactgg tctaaattaa
      541 atttacatca gcataaatgg gtcaggacgc ttttaatcac atataaaaag cgtccttttt
      601 ctcacaatca aataatcatc ggcttctata acgaaatcga tacggattag tgtgttactc
      661 gtcttcatcc tcaaaacgcg ccacgattcg ctcgccggag tggttggcac gcagctcttc
      721 tgccaccagc gcgattgcct ggccgctgct catcccttgt gccatcagtt cctggatccg
      781 ctcgacagct ttttgctgct gttcatgggt gagtgaaggt aaacctgcaa acattgttaa
      841 ctcctgctaa attgttggcg ctaattattt catgctaccc ggcacatagc cagtagagtc
      901 aggactgatg aagacgttat ctcccgctgt gattacttta ccctggcgtc aggacgccgc
      961 tgaattttat ttctcacgct taagccacct gccgtgggcg atgcttttac actccggcta
     1021 tgccgatcat ccatatagcc gctttgatat tgtggtcgcc gagccgattt gcactttaac
     1081 cactttcggt aaagaaaccg ttgttagtga aagcgaaaaa cgcacaacga ccactgatga
     1141 cccgctacag gtgctccagc aggtgctgga tcgcgcagac attcgcccag cgcataacga
     1201 agatttgcca tttcagggcg gcgcgctggg gttgtttggc tacgatctgg gccgccgttt
     1261 tgagtcactg ccagaaattg cgcagcaaga tatcgttctg ccggatatgg cagtgggtat
     1321 ctacgactgg gcgctggttg ttgaccacca gcgtcaaaca gtttctttgc tgagtcataa
     1381 tgatgtcaat gcccgtcggg cctggctgga aagccagcaa ttctcgccgc aggaagattt
     1441 cacgctcact tccgactggc aatccaatat gacccgcgag cagtacggcg aaaaatttcg
     1501 ccaggtacag gaatatctgc acagcggtga ttgctatcag gtgaatctcg cccagcgttt
     1561 tcatgcgacc tattctggcg atgaatggca ggcattcctt cagcttaatc aggccaaccg
     1621 cgcgccattt agcgcttttt tacgccttga acagggcgtg attttaagcc tttcgccaga
     1681 gcggtttatt ctttgtgata atagtgaaat ccagacccgc ccgattaaag gcacgctacc
     1741 acgcctgccc gatcctcagg aagatagcaa acaagcagag aaactggcga actcccccaa
     1801 agatcgcgct gaaaatctga tgattgtcga tttaatgcgt aatgatatcg gtcgtgttgc
     1861 cgtagcaggt tcggtaaaag taccagagct gttcgtggtc gaacccttcc ctgccgtgca
     1921 tcatctggtc agcaccataa cggcgcggct accagaacag ttacacgcca gcgatctgct
     1981 gcgcgcagct tttcctggtg gctcaataac cggggctccg aaagtacggg ctatggaaat
     2041 tatcgacgaa ctggaaccgc agcgacgcaa tgcctggtgc ggcagcattg gctatttgag
     2101 cttttgcggc aacatggata ccagcattac tatccgcact ctgactgcca ttaacggaca
     2161 aatatactgc tctgcgggcg gtggaattgt cgccgatagc caggaagaag cggaatatca
     2221 ggaaactttt gataaagtta ataagatatt acgccaactg gagaagtaag acgtggaata
     2281 ccgtagcctg acgcttgatg attttttatc gcgctttcaa cttttgcgcc cgcaaattaa
     2341 ccgggaaacc ctaaatcatc gtcaggctgc cgtgttaatc cccatcgtcc gtcgaccgca
     2401 accggggttg ttgctgactc agcgttcaat tcatctgcgt aaacacgctg gacaagtggc
     2461 attccctgga ggcgcagtcg atgacacgga tgcatcagtt atcgccgccg cgctgcgcga
     2521 agctgaagaa gaggtcgcta taccgccttc cgccgttgaa gttatcggcg tgctgccgcc
     2581 cgtcgatagc gtaacgggct accaggtaac cccagtggtc ggcattatcc cgcccgatct
     2641 gccgtatcgc gccagtgaag atgaagtctc ggcggtgttt gaaatgccgc tcgcccaggc
     2701 attacatctg ggtcgttatc accctttaga tatctaccgc cgtggcgatt cacatcgggt
     2761 atggctgtcc tggtacgaac agtattttgt atggggaatg accgcaggca taattcgtga
     2821 gctggcactg caaattggtg tgaaaccctg actatactta tctttacatc tacaaaacac
     2881 tacttgagac aatcatcgca atattagtta aatcgcggtt tttgattagt ttaattcatg
     2941 tgaatagtta agccagtcgc cgcgttccct cttacactat gcgctgttat tagttcgtta
     3001 ctggaagtcc agtcaccttg tcaggagtat tatcgtgatt agtctattcg acatgtttaa
     3061 ggtggggatt ggtccctcat cttcccatac cgtagggcct atgaaggcgg gtaaacagtt
     3121 cgtcgatgat ctggtcgaaa aaggcttact ggatagcgtt actcgcgttg ccgtggacgt
     3181 ttatggttca ctgtcgctga cgggtaaagg ccaccacacc gatatcgcca ttattatggg
     3241 tcttgcaggt aacgaacctg ccaccgtgga tatcgacagt attcccggtt ttattcgcga
     3301 cgtagaagag cgcgaacgtc tgctgctggc acagggacgg catgaagtgg atttcccgcg
     3361 cgacaacggg atgcgttttc ataacggcaa cctgccgctg catgaaaacg gtatgcaaat
     3421 ccacgcctat aacggcgatg aagtcgtcta cagcaaaact tattattcca tcggcggcgg
     3481 ttttatcgtc gatgaagaac actttggtca ggatgccgcc aacgaagtga gcgtgccgta
     3541 tcctttcaaa tctgccaccg aactgctcgc gtactgtaat gaaaccggct tttcgctgtc
     3601 tggtctcgct atgcaaaacg aactggcgct gcacagcaag aaagagatcg acgagtattt
     3661 cgcgcatgtc tggcaaacca tgcaggcatg tatcgatcgc gggatgaata ccgaaggcgt
     3721 actgccaggc ccgttgcgcg tgccacgtcg tgcgtcggcc ctgcgccgga tgctggtttc
     3781 cagcgataaa ctgtctaacg atccgatgaa tgtcattgac tgggtaaaca tgtttgcgct
     3841 ggcagttaac gaagaaaacg ccgccggtgg ccgtgtggta actgcgccaa ctaacggtgc
     3901 ctgcggtatc gttccggcag tgctggctta ctatgaccac tttattgaat cagttagccc
     3961 agacatctat acccgttact ttatggcagc gggcgcgatt ggtgcactgt ataaaatgaa
     4021 cgcgtctatt tccggtgcgg aagtcggttg ccagggcgaa gtaggtgttg cctgttcaat
     4081 ggctgctgcg ggccttgccg agctgttggg cggtagcccg gaacaggttt gtgtggcagc
     4141 ggaaattggc atggaacaca acctcggtct gacctgcgac cccgttgcag ggcaagttca
     4201 ggtgccgtgc attgaacgta atgccattgc ctctgtgaag gcgattaacg ccgcacggat
     4261 ggctctgcgc cgcaccagtg caccgcgcgt ctcgctggat aaggtcatcg aaacgatgta
     4321 cgaaaccggt aaggacatga acgctaaata tcgcgaaacc tcacgcggtg gtctggcaat
     4381 caaagtccag tgtgactaat acttcctact cgcccatctg caacggatgg gcgaatttat
     4441 tcccgcttcc tcgtctgctg taattttccc cactacactt ccactgttgc gtcaggcgtt
     4501 tgtcgccata cgcttacagg gtggcccgca tgcaaaaagc acaacggatc attaaaacct
     4561 atcgccgtaa tcgaatgatt gtttgtacga tttgcgcact cgttacgctc gcttcgaccc
     4621 tgagcgtgcg atttatttca cagcgtaact taaatcaaca acgggtagta caattcgcca
     4681 atcacgctgt agaggaatta gataaagtac tgcttcccct acaggcaggt agcgaagtct
     4741 tgcttccgct gattggtctg ccctgctctg tcgcccattt gccattacgt aaacaggctg
     4801 caaaactcca aactgtgcga tccattggcc tggtgcaaga cggcacactt tattgctcca
     4861 gcatttttgg ttatcgcaat gtgcccgtcg tggacattct ggctgaactt cctgcaccgc
     4921 aaccactttt acgactgacg accgaccgtg ccctgataaa aggcagtccg gttttgattc
     4981 aatggacgcc tgcagcgggc agtagcaatg ctggggtcat ggagatgatt aacatcgact
     5041 tactgacggc aatgctcctt gaaccacaac tgccgcaaat cagtagcgcc agcctgacgg
     5101 tggacagtcg gcatttgctc tatggtaatg ggctggtaga ttcccttccg caacctgaaa
     5161 acaatgaaaa ctatcaggtt tcttcgcaac gctttccttt taccattaac gttaatggcc
     5221 caggggctac ggcgctggca tggcactatc ttccaacaca attaccgctg gcggtgctgc
     5281 taagtttact ggtgggctac atcgcctggc tggcgaccgc ttaccgaatg agcttttccc
     5341 gcgagatcaa tctgggcctg gcgcaacatg agttcgaatt gttctgtcag cctttgctta
     5401 atgcgcgcag ccagcaatgt attggtgtag agattttgct gcgctggaac aatccacgtc
     5461 agggctggat ttcaccggat gtgtttattc ctatcgcgga agaacatcat ttaattgtgc
     5521 cactaacccg ctatgtgatg gccgaaacca ttcgtcagcg ccatgttttc ccaatgagta
     5581 gtcagtttca tgttggcatt aacgtcgcac ccagccattt tcgccgtggt gtgctgataa
     5641 aagatctcaa tcagtactgg tttagcgctc acccgattca gcaactgatc ctcgaaatca
     5701 ccgaacgcga tgccttactg gatgttgatt accggattgc ccgcgagctg catcgtaaaa
     5761 acgtcaaact ggcgattgat gacttcggca ccggcaacag ttcattttcc tggcttgaaa
     5821 cattacgtcc tgacgttctg aaaattgata agtcatttac cgcagctata ggttctgatg
     5881 cggttaactc gacggtgacc gatatcatca tcgcactggg gcaaagactg aatattgaac
     5941 tggtggcgga gggcgtagaa acgcaagaac aggcgaagta tttgcgcagt catggcgtgc
     6001 atattttgca agggtatttg tacgcacagc cgatgccgct acgtgatttt cccaaatggc
     6061 tggcgggcag ccaaccgccg cccgcccggc ataatggaca tatcacaccc attatgccgt
     6121 tacgttaacg cttactcatc ttcatcgtgc gccggttgct ctttaacaat gcgaaccaga
     6181 tcaacacgat aatcattggc ttcaatgatg gtgatatgca gtggccctac atcaatcaca
     6241 tcgcccacac ggggaatgtg accatttgcc gagatcacga ggcccgcgac cgtcgcgata
     6301 tcatcgtcat cggcaaggtg ctcaacatca agcgcctgct gcaaggcatg caaatctgta
     6361 ccgcctttta ccagccagcc gtcaccgtcg gtaatgattt ccggcgtttc gtcagcgtcc
     6421 gggaattcac ccgcaatggc ttccagcaca tccagcggcg tgaccagacc ttgtaccaca
     6481 ccaaactcgt tggtcacgat aacaaagctc ccgcgagcac gacgcagcac gcccagcagg
     6541 ttgatcggat cgagggtttc cgggacgata atcgccggag acgccgaagc aatcgccgcc
     6601 acatcaacgc cctcttccag cgccaccagc agttctttag cacgtacgat gccgataatt
     6661 tcatccagtt caccgcgaca taccgggaac aaactgtgcg gtgaggagag cagctgctcg
     6721 cggatttcat cgaccccgag attagcatca acccagctta tttcaccgcg cggcgtcatg
     6781 atcccgcgca gagaacgcga cgccagcgtc agtacgccgt taatcatgta acgttcttct
     6841 tcggcaaacg caccttccgg gatcggcatc ggcatcgggt tatcggcatc gtgctgaaca
     6901 ttggcctgac gtttcccgcc catcaaacgc aggatggcat cggcagtacg cgctcgcagc
     6961 ggcaaagtcg actggtgacg aataaagttg cgacgcgcaa tctggttaaa cacttcgatg
     7021 atgatcgaga agccaatcgc ggcatacagg taacctttcg gaatgtggaa accgaaacct
     7081 tctgccacca gactcagacc aatcattaac aggaagctca gacagagcac caccaccgtg
     7141 gggtgctggt taacgaatcg cgtcagcggt tttgatgcca gcagcataac tgccatcgca
     7201 atcactaccg ccgccatcat caccggcaaa tggttaacca tccctactgc agtaattacc
     7261 gcatccaacg agaagacggc gtcaaggatg acaatctgtg tgacgaccac ccagaaactg
     7321 gcgtagcctt taccgtggcc ggaatcatga tcgcggtttt ccagccgttc atgcagttcg
     7381 gttgttgctt tgaacagcaa gaatatgccc ccgaacaaca taatcaggtc gcgtccggag
     7441 aaggagaaat ccatgacggt aaatagcggt ttggtcagcg tgaccatcca tgaaatcagc
     7501 gacagcagcc ccagacgcat aatcagcgcc agtgataatc ccagcaaacg cgctttatcg
     7561 cgttgttttg gcggcagttt gtcagcaaga atggcgatga agaccaggtt atcgataccc
     7621 agcacaattt cgagaacaac aagcgtgagt agccccgccc aaatcgaggg gtccattaag
     7681 aattccatga caagctcctg cttaaggaat agctattcga cgccagaaat aatgcaggcg
     7741 taacgacaaa atgcaaacga aaggtgcggc ataaagtgcc agaaagacag gcgttaaaag
     7801 gcctgatgct gaaatgacgt cggtgacgat ccatactgcg ggctactgcc ctatactcca
     7861 tggttgttaa acaggagtta aacatatcag agacgcctct gatttggcaa agatttacct
     7921 tcctttgcaa acgaatgtga caaggatatt ttacctttcg aaatttctgc taatcgaaag
     7981 ttaaattacg gatcttcatc acataaaata attttttcga tatctaaaat aaatcgcgaa
     8041 acgcaggggg tttttggttg tagcccttat ctgaatcgat tcgattgtgg acgacgattc
     8101 aaaaacacat ctggcacgtt gaggtgttaa cgataataaa ggaggtagca agtgaccatt
     8161 gctattgtta taggcacaca tggttgggct gcagagcagt tgcttaaaac ggcagaaatg
     8221 ctgttaggcg agcaggaaaa cgtcggctgg atcgatttcg ttccaggtga aaatgccgaa
     8281 acgctgattg aaaagtacaa cgctcagttg gcaaaactcg acaccactaa aggcgtgctg
     8341 tttctcgttg atacatgggg aggcagccca ttcaatgctg ccagccgcat tgtcgtcgac
     8401 aaagagcatt atgaagtcat tgcaggcgtt aacattccaa tgctcgtgga aacgttaatg
     8461 gcccgagatg atgaccctag ctttgatgaa ctggtggcac tggcagtaga aacaggccgt
     8521 gaaggcgtga aagcactgaa agccaaaccg attgaaaaag ccgcgccagc acccgctgcc
     8581 gcagcaccca aagtggctcc aactccggca aaaccaatgg gaccgaacga ctacatggtt
     8641 attggccttg cgcgtatcga cgaccgtctg attcacggtc aggtcgccac ccgctggacc
     8701 aaagaaacca atgtctcccg tattattgtt gttagtgatg aagtggctgc ggataccgtt
     8761 cgtaagacac tgctcaccca ggttgcacct ccgggcgtaa cagcacacgt agttgatgtt
     8821 gccaaaatga ttcgcgtcta caacaacccg aaatatgctg gcgaacgcgt aatgctgtta
     8881 tttaccaacc caacagatgt agagcgtctc gttgaaggcg gcgtgaaaat cacctctgtt
     8941 aacgtcggtg gtatggcatt ccgtcagggt aaaacccagg tgaataacgc ggtttcggtt
     9001 gatgaaaaag atatcgaggc gttcaagaaa ctgaatgcgc gcggtattga gctggaagtc
     9061 cgtaaggttt ccaccgatcc gaaactgaaa atgatggatc tgatcagcaa aatcgataag
     9121 taacgtattg tgttgattat cattcagttt tcacacttta gtcttacgta aacaggagaa
     9181 gtacaatgga gattaccact cttcaaattg tgctggtatt tatcgtagcc tgtatcgcag
     9241 gtatgggatc aatcctcgat gaatttcagt ttcaccgtcc gctaatcgcg tgtaccctgg
     9301 tgggtatcgt tcttggcgat atgaaaaccg gtattattat cggtggtacg ctggaaatga
     9361 tcgcgctggg ctggatgaac atcggtgctg cagttgcgcc tgacgccgct ctggcttcca
     9421 tcatttctac tattctggtt atcgcaggtc atcagagcat tggtgcaggt atcgcactgg
     9481 caatccctct ggccgctgcg ggccaggtac tgaccatcat cgttcgtact attaccgttg
     9541 ccttccagca cgctgcggat aaggctgctg ataacggcaa cctgacagcg atttcctgga
     9601 tccacgtttc ttctctgttc ctgcaagcaa tgcgtgtggc tattccggcc gtcatcgttg
     9661 cgctgtctgt tggtaccagc gaagtccaga acatgctgaa tgcgattccg gaagtggtga
     9721 ccaatggtct gaatatcgcc ggtggcatga tcgtggtggt tggttatgcg atggttatca
     9781 acatgatgcg tgctggctac ctgatgccgt tcttctacct cggcttcgta accgcagcat
     9841 tcaccaactt taacctggtt gctctgggtg tgattggtac tgttatggca gtgctctaca
     9901 tccaacttag cccgaaatac aaccgcgtag ccggtgcgcc tgctcaggca gctggtaaca
     9961 acgatctcga taacgaactg gactaacagg tgagcgaaat ggttgataca actcaaacta
    10021 ccaccgagaa aaaactcact caaagtgata ttcgtggcgt cttcctgcgt tctaacctct
    10081 tccagggttc atggaacttc gaacgtatgc aggcactggg tttctgcttc tctatggtac
    10141 cggcaattcg tcgcctctac cctgagaaca acgaagctcg taaacaagct attcgccgtc
    10201 acctggagtt ctttaacacc cagccgttcg tggctgcgcc gattctcggc gtaaccctgg
    10261 cgctggaaga acagcgtgct aatggcgcag agatcgatga cggtgctatc aacggtatca
    10321 aagtcggttt gatggggccg ctggctggtg taggcgaccc gatcttctgg ggaaccgtac
    10381 gtccggtatt tgcagcactg ggggccggta tcgcaatgag cggcagcctg ttaggtccgc
    10441 tgctgttctt catcttgttt aacctggtgc gtctggcaac ccgttactac ggcgtagcgt
    10501 atggttactc caaaggtatc gatatcgtta aagatatggg tggtggcttc ctgcaaaaac
    10561 tgacggaagg ggcgtctatc ctcggcctgt ttgtcatggg ggcattagtt aacaagtgga
    10621 cgcatgtcaa catcccgctg gttgtctctc gcattactga ccagacgggc aaagaacacg
    10681 ttaccactgt ccagactatt cttgaccagt taatgccagg cctggtacca ctgctgctga
    10741 cctttgcttg tatgtggctg ttgcgcaaaa aagttaaccc gctgtggatc atcgttggct
    10801 tcttcgtcat cggtatcgct ggttacgctt gcggcctgct gggactgtaa gactgttgta
    10861 cactaccggg gccttttggc cccgtttttt tatctggagg attaatgaca atcacggacc
    10921 tggtactgat tcttttcatc gccgcactcc tggccttcgc gatctacgat cagttcatca
    10981 tgccccgccg taacggcccc accctgctgg caattccttt gctccggcgt ggtcgcatcg
    11041 atagcgttat cttcgtcgga ttgattgtca ttcttatcta taacaacgtc acgaatcatg
    11101 gcgcgctaat aacaacatgg ttattaagtg cactggctct gatgggtttt tatatattct
    11161 ggatccgcgt tccgaagatc atctttaaac aaaaaggttt tttcttcgcc aatgtctgga
    11221 ttgaatatag ccgaatcaaa gcgatgaact tgtcggaaga tggcgtgttg gtgatgcaat
    11281 tagaacagcg ccggctgtta atccgcgttc gaaatatcga cgatttggaa aaagtttata
    11341 agcttctcgt ttcaactcaa taagttatga atttagccaa agc
//
LOCUS       DAZFJL010000141        11372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFJL010000141 DAZFJL010000000
VERSION     DAZFJL010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.143"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            31..393
                     /locus_tag="ACHJ8C_003874"
     CDS             31..393
                     /locus_tag="ACHJ8C_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553657.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMGKGREE"
     gene            complement(496..954)
                     /gene="ybbJ"
                     /locus_tag="ACHJ8C_003875"
     CDS             complement(496..954)
                     /gene="ybbJ"
                     /locus_tag="ACHJ8C_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO5553658.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLLPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGQRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGSHVEVIAIEGITLIIRAVIA"
     gene            complement(951..1868)
                     /gene="qmcA"
                     /locus_tag="ACHJ8C_003876"
     CDS             complement(951..1868)
                     /gene="qmcA"
                     /locus_tag="ACHJ8C_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO5553659.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2014..2691
                     /gene="fetA"
                     /locus_tag="ACHJ8C_003877"
     CDS             2014..2691
                     /gene="fetA"
                     /locus_tag="ACHJ8C_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO5553660.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLCAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDTILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2678..3457
                     /gene="fetB"
                     /locus_tag="ACHJ8C_003878"
     CDS             2678..3457
                     /gene="fetB"
                     /locus_tag="ACHJ8C_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO5553661.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDACLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3520..4374)
                     /gene="cnoX"
                     /locus_tag="ACHJ8C_003879"
     CDS             complement(3520..4374)
                     /gene="cnoX"
                     /locus_tag="ACHJ8C_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO5553662.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4435..5244)
                     /gene="ybbO"
                     /locus_tag="ACHJ8C_003880"
     CDS             complement(4435..5244)
                     /gene="ybbO"
                     /locus_tag="ACHJ8C_003880"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO5553663.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5234..5857)
                     /gene="tesA"
                     /locus_tag="ACHJ8C_003881"
     CDS             complement(5234..5857)
                     /gene="tesA"
                     /locus_tag="ACHJ8C_003881"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO5553664.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNETFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            5828..6514
                     /gene="ybbA"
                     /locus_tag="ACHJ8C_003882"
     CDS             5828..6514
                     /gene="ybbA"
                     /locus_tag="ACHJ8C_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO5553665.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVRLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6511..8925
                     /gene="ybbP"
                     /locus_tag="ACHJ8C_003883"
     CDS             6511..8925
                     /gene="ybbP"
                     /locus_tag="ACHJ8C_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO5553666.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQIAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSIERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVTVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNGDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQSKVFDFPWEPDWRLWIVLPCSGALLLSLCGGWLGARLLKGKAL
                     FRQFAG"
     gene            complement(9166..10260)
                     /gene="mnmH"
                     /locus_tag="ACHJ8C_003884"
     CDS             complement(9166..10260)
                     /gene="mnmH"
                     /locus_tag="ACHJ8C_003884"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO5553667.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGICYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPHGILCCARGGQ
                     RSHIVQRWLHDAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENMLAAEMLKTDAHQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQATIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            complement(10329..11255)
                     /gene="allS"
                     /locus_tag="ACHJ8C_003885"
     CDS             complement(10329..11255)
                     /gene="allS"
                     /locus_tag="ACHJ8C_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO5553668.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLCQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVARLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANIEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMLDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
BASE COUNT         2593 a         2991 c         3059 g         2729 t
ORIGIN      
        1 aagaggttta cggcaggcag gaaaatttaa atgaatgaat ttgagaagat ttttaatgaa
       61 atgaatctgg accgggcatt gctaccgatc ctttttcgtt cgaaccggtc gacagtgtgg
      121 aagtatttaa gcggcgatag tacggctcct gcctctgcca tgtcgctgat tatgctgttg
      181 cagttgatcc agaaacgaaa ccccgatctg ttagccgaat ggttaacgct ttctgatttc
      241 accattccgc cagaagttta cctggaccaa cctgattact ggaaagggtg ggtgtatacc
      301 caacacaagg tcaataaaaa cgtcctggaa tatctaaaaa aacactatcc ggatgaagac
      361 caaaaaagta tgggtaaagg ccgggaagag taattttgcc tctactcggc tatatgccgg
      421 atgcggcgca agcgccttat ctggcctacg attcgggcat attttgtagg catgataaga
      481 cgcgccagcg tcgcatcagg cgatgaccgc acggataatc agcgttattc cttctatcgc
      541 aatcacctca acatgcgaac cagcgccgag atcctcgctg gcgctgacag gccatgaact
      601 gtcaccgacg cgcatatgac cgcgcccgtt gaccagcgga gattccagaa caaaacgttg
      661 gccaatcaac tgctgcccgc gctggtttaa atgactgtcg ctgtgctttt gttcgcgcac
      721 ccgccgcgac aaccatttcc accacagcca ggcggcgagc agcgtcagga cggcaaacat
      781 taccccttgc cactcccaac ccagcggcag caaccagacc accagaccgg taatcaccgc
      841 tgccacgccg ctccacaaca gataaccatt gccgcccaac atctcggctg ccagcagcaa
      901 accgccgaga cttagccaga aaatatgtgg atgaacgacc attaactcca tcatggctga
      961 gtccgtttgt tggcgctgtc tttcaccagc tcagcaatcc cggcaatcga ccccatcagg
     1021 ctgctggcct ctaatggcat catcaccact ttgctgttac tggacgaacc gatctgctgt
     1081 aacgcttcgg tgtatttctg tgcgacgaag tagttcaccg cctgaatatc accggaggca
     1141 atggcttcag acaccatttt agtggcgcgg gcttctgctt cagcggaacg ttcacgcgct
     1201 tcagcctgta aaaacgccga ctgacgttca ccttccgctt tcaggatttg cgactgtttt
     1261 tcaccttcgg ctttgaggat ttctgcctga cggatccctt ccgcttcaag gatgtaagcg
     1321 cgtttggtac gttccgcttt catctgcgcg ttcattgaag agataagctc tgccggtggg
     1381 cgcacgtcgc gaatttcaat acgggtgact ttaatccccc acgggttggt ggcctcatcg
     1441 acaatacgca gcaggcgtga gttgatgctg tcgcgctgag agagcatttc atcaagttcc
     1501 attgaaccca acacggtacg gatgttagtc atggtcaggt tgatgatcgc cagctccaga
     1561 ttgctgactt cataagcggc gcgcggggca tcaatcacct gaataaagca cacggcgtcg
     1621 atggtaacgt tggcgttatc tttcgagata acttcctggg aagggatgtc gagcacttgc
     1681 tccatcatat tgatcttgcg accaatgcga tccataaacg gcaccaccag actgagcccc
     1741 ggctgtaacg ttttggtata gcgaccaaaa cgttctaccg tccattgata gccctgcggc
     1801 acgattttga cgcccgcgcc gacaatgacc agcgcgacaa aaatgagaat agggataaag
     1861 ataagcatcg gaaaaacctc ctgttgtacc gtccataatc agcaaaattg ctgcttgatt
     1921 aaacaaatta tacctgatta ctgaaagaga gttccccctt attcctgcga aggataaact
     1981 gtttttagta aaaatcagaa aaagggaaca gcgatgcagg aaaatagtcc tttgcttcag
     2041 ctacaaaacg taggatatct ggcgggtgat acgaagattc ttaataacat caatttttcg
     2101 ctgtgtgctg gcgaatttaa gttaattacc ggtccttctg gttgtggcaa aagtacactg
     2161 ctaaaaatag ttgcttcatt gatcagccca accagcggaa cgttactgtt tgaaggtgag
     2221 gatgtcagca cactaaagcc agaaatctac cgtcagcaag tctcttactg cgcccagacg
     2281 ccgacgctgt ttggcgatac ggtatacgat aacctgattt ttccctggca gatccgtaac
     2341 cagcagcctg acccggccat ttttctcgat tttcttgaac gcttcgcttt gccggacacc
     2401 attttgacga agaatatcgc cgagctatct ggtggtgaaa aacaacgcat ctcattgatt
     2461 cgtaacctgc aatttatgcc gaaggtttta ttgctcgacg aaatcaccag tgcgctggat
     2521 gaaagtaata aacataacgt taatgagatg atccaccgtt atgtgcgcga gcaaaatatt
     2581 gccgtgctgt gggtgacaca cgataaagac gaaattaatc atgcggataa agtgattaca
     2641 ctgcaaccgc atgccggaga aatgcaggaa gcacgctatg aactcgcata atattactaa
     2701 cgaatcatta gcactggcat tgatgctggt ggtggtggca atcttaatta gccataaaga
     2761 aaaactggcg ctggagaaag atattctctg gagcgtcggg cgcgcgataa tccagctgat
     2821 tattgtcggc tatgtgctga agtatatttt cagcgtggat gatgcctgtc tgacattatt
     2881 gatggtgtta tttatctgct ttaatgcggc gtggaacgcg caaaaacgca gtaaatatat
     2941 tgctaaagct tttatctcat cgtttattgc cattacggtc ggagcgggaa ttaccctggc
     3001 ggtactgatc ctctccgggt cgattgaatt tatcccgatg caggtgatcc ctatcgccgg
     3061 gatgatagcc ggcaacgcca tggtagcggt ggggttgtgt tacaacaatt tagggcaaag
     3121 ggtcattagc gaacagcaac agattcagga gaaactgagt cttggcgcga cgccgaaaca
     3181 ggcttcagcg atattgattc gcgacagtat tcgcgcggct ttaattccga cggtcgattc
     3241 agcaaaaacg gtaggcttag tgagtttacc aggaatgatg tccgggctga tatttgccgg
     3301 gattgatccg gtgaaggcga ttaaatatca gattatggtg acctttatgc tgctctcaac
     3361 cgccagcctg tcgaccatta ttgcctgcta tttaacctat cgtaagtttt acaattcgcg
     3421 ccaccaattg gtggtgacgc aattgaagaa gaaatgatgc gatgccggat gcgcagcatc
     3481 gcatccggcg ttgtggttca tgtgccgctc aacaaaacat caatacaaca acgcatacag
     3541 ctggcggcga tacttcgacg ccagtgcatc acccgtaccc agcgcagcga ggatctcctg
     3601 gaatgtttta cgtgtctgac catctgcggc ggtgagatct ttacgcagat gcccaaacag
     3661 taactccagc gcctcttcat tgcgcccaac ctggtgcagt tgcagtgcca gttgcgtcgc
     3721 cagtgcggca tcttctggat tctccgccac ctgctgttgc aactgttgaa tttccggcgt
     3781 atcagccgcc tgcttcagca gttcgatttg cgccaccagc ccctgatagc gggtgtcctg
     3841 atcctgcaac ggaatggttt tcagcacggc ttctgcatct tcagaacggt tcagcgcaat
     3901 cagcgtttct gccaacagca ggccgatttc cccgttctga ttcgacaact gccaggcgtc
     3961 tttcagcaat ggcagggcgt cggtgtaatt gccttcctgc atcagttgca tcgcctgctg
     4021 cgctttcagc tcttcttcac gcggcagcac tttatccagc agggcgcgga tcgcctcttc
     4081 cggttgcggc ccctggaagc cgtctactgg ttgcccgttc tggaacagat atacggtcgg
     4141 aatcgcgcgc agaccaaact gggcggcaat catctgctcc gcgtcgcagt ccagcttcgc
     4201 cagaataaat tgcccgttgt actgcgccgc gaggctttcc agaattgggg ttaactgcaa
     4261 acagtgctgg ctacgttcag accaaaaata gaacagcacc ggagtggtca tcgactgttc
     4321 aagaacctgt tgcaggttag attcgttaat gttgacaata ttttctacgg acatggagtc
     4381 gctctctgtt gtcgatattt tctttgacat gggggcttaa gcgcgcgctt caactcaccc
     4441 ctgcaatatt ttgtccatca cgcgccccgg cagcaggcgc ttaagcacca ttaccgccca
     4501 ggtcaccagc gtcaccggat agcgcatctt cggcttctcg ctaataaaag catggcgcac
     4561 tttgtcgacc accgcttccg gtcccaacgt aaagcgggcg gcgatgccag gattttcgac
     4621 tggtttatca ctttgcgtct gattgacgtt gtcggtgaag cgagtacgaa tgggaccagg
     4681 ttcgatcagg ctgactttaa ttccgctgtg gcgcagctcc atgcgcagtg cgtctgacca
     4741 cgcctccagc gcatatttac tggccgcgta agcgccacga cccggtgtgg agattaatcc
     4801 catcaccgat gaggtcatca caatacgccc ttcaccgtgc ggcaacatcg cgggtaatag
     4861 gcgcatggtg agctggtgtg cgccgaaaaa gttggcggaa aactgctgtt ccatctgcgc
     4921 acggctgatg gtggaaaggg ggccatacat gccgaatccg gcattgttaa agatcccata
     4981 cagacaatta tcggtcaggg cgatcacttc gtcggctgcg cgatcaacac tttctggcga
     5041 atccagatcg atcaacacgc cggtaaatcc catgctgttc atgcgctcaa catcatccgg
     5101 tttccggcaa cctgccagca catgaaaacc ctggcgtttt aattcgagtg cgctttccag
     5161 gccaatacca ctggaacatc cggtaattaa gaccgatttt tgcataactt tacctgtcag
     5221 gatctccgtt gctttatgag tcatgattta ctaaaggctg caactgcttc gccatccagt
     5281 cggcaataaa cggctgggcg tcgcggttgg gatgaatacc gtcatcctgc atccattgtg
     5341 gcttgaggta gacctcttcc ataaaaaagg gcagcagcgg aacatcaaac tctttggcga
     5401 gtttgggata aatggcgcta aaggtttcat tataacggcg accatagttt gcaggcagac
     5461 gtatttgcat taacaatggt tcagcgttgg cggctttgac atcctgcaaa atctggcgca
     5521 gcgtttgctc ggtttgctgt ggttgaaaac cacgcaaacc gtcattgccg cccagttcaa
     5581 ccagcaccca gcgcggctga tgctgtttca gcagagccgg aaggcgcgcc agcccttgtt
     5641 gcgaggtgtc accgctgatg ctggcattga ctaccgacgt tttactctgc cacttatcat
     5701 tcaacaaggc aggccaggcc gcgctggcag acattcgata cccggcgctc aggctatcac
     5761 ccagaatcaa taacgtgtcc gctgcggcgg cacggaaggt taacaggacc aggaacagga
     5821 agggcaaatg ccagcggaaa acattgttga agttcatcat cttaagaagt ccgtcggtca
     5881 gggggagcat gaactctcca tcctcaccgg agttgagctg gttgtcaaac gtggcgagac
     5941 catcgcactg gtgggcgagt cgggatcggg taagtcaacc ttgctggcga tcctcgccgg
     6001 gctggatgac ggcagcagtg gcgaagtgag actggtggga caaccgctac ataatatgga
     6061 cgaagaagcg cgggcaaagt tgcgcgcgaa gcacgtaggc tttgtttttc agtcatttat
     6121 gttaattcct acccttaacg cgctggaaaa cgtcgagctt ccggcgctgc tgcgcggtga
     6181 gagtagcgca gaaagtcgta acggggcgaa agcgttgctc gaacagttag ggctgggtaa
     6241 acgtcttgat catcttccgg cacagctttc tggcggcgaa cagcaacgag tggcgctggc
     6301 gcgagccttt aatggtcgac ctgatgtgct gtttgccgac gaacccaccg gtaaccttga
     6361 ccgccagacg ggcgataaaa ttgccgatct gcttttttcc ctcaaccgtg agcatggcac
     6421 cacgttgatt atggtgaccc atgacctgca actggcggca cgctgtgacc gctgcttacg
     6481 gctggtgaac gggcagttgc aggaggaagc atgattgcac gttggttctg gcgcgaatgg
     6541 cgctcgccgt cgctattaat tgtctggctg gcgcttagcc tggcggtggc ctgcgtgctg
     6601 gcgctgggca atatcagcga tcgcatggag aaaggtttaa gccagcaaag ccgggagttt
     6661 atggcgggcg atcgggcgtt gcgcagttcg cgcgaagtgc cgcaagcatg gctggaggaa
     6721 gcgcaaaagc gcggcctgaa agtcggcaag caactgactt tcgccaccat gacatttgca
     6781 ggcgacacac cgcagctggc gaacgtcaaa gcggtggatg atatctaccc gatgtatggc
     6841 gatctgcaaa ctaatccccc tggcctgaaa ccgcaggcgg gcagcgtatt gctggcccca
     6901 cgcctgatgg cactgcttaa cctgaaaacg ggcgacacca ttgacgtggg cgatgccacc
     6961 ttgcggattg ccggagaagt gattcaggaa ccggattccg gttttaatcc tttccagata
     7021 gccccacgcc tgatgatgaa tctggcggat gttgataaaa ccggagccgt gcagccgggg
     7081 agtcgggtca cctggcgtta taaattcggc ggcagcgaga accagctcga cggctatgag
     7141 aaatggttgt taccccagct taaacctgaa caacgctggt acggtctgga acaggacgaa
     7201 ggcgcgctgg ggcgatcgat agaacgttcg caacagttcc tgctgctttc ggcgcttctg
     7261 accttgctgc tggcggtggc ggcggttgcg gtggcgatga atcattactg tcgcagtcgc
     7321 tacgacttgg tggcgatcct caagacgctg ggggcagggc gagcgcaact gcgtaagtta
     7381 atcgtcggtc agtggttgat ggtgctggcg ctttcagccg ttaccggtgg ggcgataggc
     7441 ctgttgttcg aaaacgtgtt gatggtgctg ctcaagcccg ttctgcctgc tgcactaccg
     7501 ccagccagcc tctggccgtg gctgtgggcg ctcggcacca tgacggttat ttcgctgctg
     7561 gtggggctac gaccgtaccg actgttactg gccacgcagc ctttacgcgt attacgtaat
     7621 gatgtggtag cgaacgtctg gccgctgaag ttttatctgc cgattgtcac cgtggtggtt
     7681 gtgctgctgc tcgccggact gatgggtggc agtatgctgc tctgggcggt gctggcgggc
     7741 gcggtagtac tggctttgct gtgcggtgtg ctgggctgga tgctgctgaa tgtacttcgc
     7801 cgcatgacgc tgaaatcgct acctctgcgc ctggcggtta gccgcctgtt acgtcagccg
     7861 tggtcaacgt taagccagct ttcggcattt tcgctctcct ttatgctgct ggcactgttg
     7921 ctggtgttgc gtggcgatct gctcgaccgt tggcaacaac aactgccgcc agaaagcccg
     7981 aactactttt taatcaacat cgctacggaa caggttacgc cgctgaaagc gtttctcgcg
     8041 gaacatcaga tagtcccgga atcgttttat ccggtggtgc gggcgcggtt gacggcgatt
     8101 aacgacaagc cgacagaagg caatggagat gaggcgctca accgcgagct gaatctcacc
     8161 tggcaaaata cgcggcctga tcataacccg atcgtcgccg gtaactggcc gccaaaagcc
     8221 gatgaagtgt cgatggaaga ggggctggcg aaacgcttaa acgttgccct cggcgatacc
     8281 gtgactttta tgggcgatac ccaggagttc cgcgctaaag tgaccagcct gcgcaaagtg
     8341 gactgggaaa gtctgcggcc taatttctat tttattttcc cggaaggggc attagacggg
     8401 caaccgcaga gctggcttac cagtttccgc tgggagaatg gcaacggcat gttgacgcaa
     8461 ctcaaccgcc agttcccgac cattagcctg ttagacattg gcgcgatttt aaaacaggtc
     8521 ggtcaggtgc tggagcaggt aagtcgggcg ctggaagtga tggtggtgct ggtcaccgcc
     8581 tgcggtatgt tgttgttgct ggcacaggtg caggtgggaa tgcgtcagcg tcatcaggag
     8641 ctggtggtgt ggcgcacact tggtgcgggg aaaaaactgc tgcgcacgac attgtggtgt
     8701 gagttcgcca tgctcgggtt tgtatccggc ctggtggccg caattggtgc ggaaacggca
     8761 ctggcggtac tgcaatcgaa agtgtttgat ttcccgtggg agccagactg gcgattgtgg
     8821 attgttctgc cgtgcagcgg tgcgctactg ctgtcgcttt gcggcggctg gctgggtgcg
     8881 cgactgctta agggtaaggc gctgttcagg cagtttgcgg ggtgatgaca ggtgttagat
     8941 tgcctgatgc gctacgctta tcaggcctac gaagatcgtg caatatattg agttttcgaa
     9001 attttgtacg ccggataagg cgttcacgcc gcatccggca gttgtgcgcc gatgccagat
     9061 gcgacgcagg cgcgtcttat catgcctacg aaacagcact cacctgtagg ctggataagg
     9121 cgttcacgcc gcatccggca tgaacagcgt gcacagaatc ccccatcact gcgccttaac
     9181 ccattccgcc acttccgccc actcaccgcg aaagacaact ttttccgctt ttttctcaag
     9241 ctgatagcga tacatcgggt cgtaatattc ttcaagtaac ggcacaagcc aggccagatg
     9301 accgtcggtg ctgccggtgg tgagttgcgt tgtcagcgcc gcatccagcc ttgcagccag
     9361 ttcgttatag cgttgtagtc ccagccgacg tttaatcgcc gaaagcccgt gatgcaggta
     9421 ttcgcaatac tcctgccagc cctgttcgtc gccataagcg tgggtaaaat catgatgcat
     9481 acgcaagaaa tactcttcgt tcaggcgttc aagacggatc tcaaacggat cttctaccac
     9541 cgcaatcgtc gcctgagtca ttcgttcgcg cagacattcc ggcaggtgat tcgaaccgat
     9601 catccggctt tcatcttcca gcacccacag gcgcaaatcc tgatgggcgt cggtttttag
     9661 catttcggca gccagcatgt tttcaaaact cgcctggcta agttgtggtt gtaaggtgcg
     9721 accaaacgcc gaaccgcgat gacgcgccaa cccttccaga tcaacaccgt tcggctgttg
     9781 ctgcactaac agcgttttac cgctgccggt acaaccgcca atcagcacga tcggtttttg
     9841 tgccagttca atagtcgcct gaatcgcggt ctggcgcagt gccttataac cgccttccac
     9901 cagcggataa tcaatccccg cgtcatgcag ccagcgttgc acaatatgcg agcgctgacc
     9961 gccacgggca cagcagagaa taccgtgcgg attttgcagg cacgctgccc gccaggcgtc
    10021 catgcgctgc tgacgaattt cacccgccac cagtttatgc cccagtgcca gcgccgcgtc
    10081 tgacccttgc tgtttatagc agatgccaac ggcggcgcgt tcatcgttat tcattaacgg
    10141 caggttgata gcggcgggca ttgcgccttg ctcaaactcg atgggggcgc gaacatcaat
    10201 aatgggcgta tcagcaatca gcagggcacg atagtcctgt tccgtgtgtc tctcttgcat
    10261 agtaaaaagt gaacctcaaa tcagcttgcg cgctatttta cgcgccaacg tgcaaggaaa
    10321 cttgattttt aactgcgtgg gttgccgaaa atttctaaaa atccgctgat ttccggcctg
    10381 cgctgggtaa acaaggtcac aatatcttct accgctttgc cgctgccaaa tttgcgccat
    10441 gccagactca atggcgaagg agggcgcatc gttgggatta cccggctgac cagttgttga
    10501 ttatcgagca ttgactggca aagtgatttt ggcaaaaaac caatgcccac gcccgccaga
    10561 tgggcggcga ttttcgtttc catatcggga acaataatct ctttttgccc cggcaatcgc
    10621 caggcgacgc gtttggttaa tgtacgggcg ctgtcttcaa tattgaccgc cggaaagcgc
    10681 cgcaactgtg cttctgttag cggctcttca atgttcgcca gcggatgatc cgccgccatg
    10741 acaaagcgcc attgcaccga tcctaaggga tcaagactaa aggtatttgc cagcgcctcg
    10801 gttcccgtga cgccgatagc cagtgaaaaa ccttcgtaca atagcgagtc ccagacgccc
    10861 atatagattt gtcgggagat gtgaaattgg gtaaacgggt aacgttcatt tagccacgcc
    10921 agcagcctgg caacggcctg ggggttatag agcaggttgt tgatgacgat attcacctgg
    10981 cgttccacgc catcattcac ctgttgtaac tcgctgggca tactttccag ccagctcagc
    11041 cagtctctgg cctgacaaag tagatgctcg ccagccgccg tcaacgtcac gctgcgagtc
    11101 gtgcggaaaa acagcgccac tccggtattc tcttccagaa gtttaatgcg ataactgatc
    11161 gtcgccgtgg ttttacataa tcgttctgcc gcttttgaaa aactacctgt ttcagcaacc
    11221 gcaatgaaag tccgcaaggt ttctggatcg aacatcttca ggtatcccct tttaaatccg
    11281 caagttgcgt gattttctta tcctctgatt tatcagtatt tttacatgat aaccctgttc
    11341 aatttgtgga ctaaatctag ttttggaaaa at
//
LOCUS       DAZFJL010000142        11371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZFJL010000142 DAZFJL010000000
VERSION     DAZFJL010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.144"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            108..5426
                     /locus_tag="ACHJ8C_003886"
     CDS             108..5426
                     /locus_tag="ACHJ8C_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO5553669.1"
                     /translation="MQRKTLLSACIALALSGQGWAADITEIETTTGEKKNTNVTCPAD
                     PGKLSPEELKRLPSECSSVVEQNLMPWLVTGAATALITTLAIVELNDDDDHHRNNSPL
                     PPTPPDDDSDDTPVPPTPGGDEIIPDDGPDDTPTPPKPISFNNDVILDKTEKTLTIRD
                     SVFTYTENADGTISLQDSNGRKATINLWQIDETNNTVALEGMSADGATKWQYNHNGEL
                     VITGDNTTVNNTGKTIVDGKGATGTEIAGNNAVVNQDGELDVSGGGHGIDITGDSATV
                     DNKGGMTVTDPDSIGIQIDGDKAVVNNDGDSAISNGGTGTSITGNEATANNTGKTTVD
                     GKDSTGTEIAGNNATVTQEGELTVSSGGRGIDITGNNAKVDTKGKMTITGTDSVGVSI
                     NGDSATLTNTGDIDVSNSAAGFSLVTNEGIISLAGSMKVGDFSTGMALSGDNNSVTLA
                     AKDINVTGQKATGVNISGDSNTVDITGNILVDKDQTADNAVDYFYDPSVGVNISGNSN
                     TVSLDGKLTVIADSELTSRKYMEFDGSQENISGLTVSGDGNTVNLNGGIQFVGEKNAL
                     ADGSTIADKRSYFGKTPLVSVDGQSKVYLNGDSTISGSLPLGYANILQLSNKAALEIG
                     SDATFSMQNISVYEHYFAQTPQIIKVDTGSQVVNNGDVDIWNISFAGIWGENSTGINN
                     GNITLSQYDYSSPETPFSEPDHMAFLSSSGGSAVNNGTITAKVMEQHSVLDMGAAAGV
                     TDPRVFNNSVSSMMGMEAYGKGTVLNSENGVIDMYGRGNIGMLAIDNSVANNAGKITL
                     DTLWVDQNDTTTLRTDLPSNTAIDYGVGMATGTNSGGGARSNGVATNQQGGVITVYNA
                     GAAMAAYGASNMVINQGIINLEKNGNYDGGLGANMLVGMAVYNRGTAINDKTGVININ
                     VDTGQAFYNDGTGTILNYGEINLLGSPMDSADSHMGAIPENLDLLTALTGSGETDMRT
                     ASSGGFVTTKALANYGNETLNSNVAAKAWLYNQDKANLTINGELSIGQGLENSGLLDS
                     DTISAAANVYNRASGSIITDQLSLTGSNSFFNEGNFSGSVAGSSYKQNVVNTGTMAVM
                     ADGKSLISGSFLLYNEAGATLSNSSSAVSGGENAIVNVTRTGDSLAQVNRGTITAVNG
                     YSAIKTASTGSNSNGKWIWNTDTGVISGVNPNAPLIDLGRGYNFANAGTINVQGDGAV
                     AISGGTTSYTVQLVNSGTINVGTAQGKADGTNGTGLIGIKGNGSDTTINNAQSGVINV
                     YADNSWAFGGKTKAIINNGEINLLCDTGCDIYAPGTTGTLNDHNSTTDIIVPAATSTP
                     TQGSVPTVPADSSAQQKLTNYTIGTNSDGTSGMLKANNLVISDNVKVNTGFSAGTADT
                     TVVINDVFKGENISGAENISSSTVMWNAQGSTDASGNVDVTMTKNAYTDVVTDSSVNN
                     VAQVLDSGYTNNDLYTSLNVGTTAELNSALKQISGSQATTVFNEARVLSNRFSMLSDA
                     APEVANGLAFNVVAKGDPRAELGNNTQYDMMALRKSMTLTEHQNLSLEYGIARLEGNG
                     SDTVGDNGVTGGYSQFFGLKHQMAFDNGMSWNNALRYDVHNLDSSRSIAYGDVNKTAD
                     ANVKQQYLEFRSEGAKTFELREGLNVTPYAGVKLRHTLENGYQERNAGDFNLSMNSGS
                     ETAVDSIVGLKLDYAGKEGWSANATLEGGPNLSYVKSQRTASISGAGSQRFNIDDGQS
                     GGGFNSLATMGVKYSSQESALQVDAFHWKEDGISDKGVMLNFKKTF"
     gene            5646..6512
                     /gene="pdxI"
                     /locus_tag="ACHJ8C_003887"
     CDS             5646..6512
                     /gene="pdxI"
                     /locus_tag="ACHJ8C_003887"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO5553670.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIVHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVATSLNATPMQVALAWLLQRSPNILLIPGTSSVGHLRENMASDELVLTDDV
                     LATLDSISQKAG"
     gene            complement(6553..7158)
                     /gene="azoR"
                     /locus_tag="ACHJ8C_003888"
     CDS             complement(6553..7158)
                     /gene="azoR"
                     /locus_tag="ACHJ8C_003888"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO5553671.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     TNPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            7359..11261
                     /gene="hrpA"
                     /locus_tag="ACHJ8C_003889"
     CDS             7359..11261
                     /gene="hrpA"
                     /locus_tag="ACHJ8C_003889"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO5553672.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDHAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPADYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELIIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEDFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAIDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EVRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         3004 a         2712 c         3059 g         2596 t
ORIGIN      
        1 attaggtaac aatctactgg caatattgga cgtcctctat gttttaaata atcaattggt
       61 cgggtcagtg tatccgacct tctttatatt cgccagaatg atttattatg caaaggaaaa
      121 ctctattgtc ggcctgtatt gcattagctc tgagtggtca gggttgggcg gcagatatta
      181 cagaaataga aaccaccaca ggtgaaaaga aaaataccaa tgtgacttgt ccagcagatc
      241 ctggaaaact cagtccagaa gagcttaaac gcttaccctc tgaatgctcc tctgtagtcg
      301 aacagaacct gatgccatgg cttgtcacag gtgctgctac tgcgttaatc acaaccttag
      361 ccatagtgga actaaacgac gatgatgatc atcatcgcaa taattctcca cttccaccaa
      421 ctccacctga tgatgattca gacgacactc cagttcctcc aactcctggc ggagatgaga
      481 taataccaga cgatggtccg gatgataccc cgacgcctcc caaaccgatt tcgtttaata
      541 atgacgttat cctcgataaa acagaaaaaa cgttaactat tcgcgattca gtttttactt
      601 ataccgagaa tgctgacggg actatttctc tgcaagatag caatgggcgt aaggcgacta
      661 ttaatctttg gcagattgat gaaacgaata acacggttgc ccttgaaggg atgagcgcag
      721 atggtgcaac gaagtggcaa tataatcaca acggtgagct cgttattacg ggcgacaata
      781 ccacagtaaa caacactgga aaaaccatcg tcgacggcaa aggcgccacc ggaactgaaa
      841 tcgcgggtaa taacgcggtg gtgaaccagg acggcgagct ggatgtgagc ggcggtggtc
      901 acggtattga tatcaccggc gacagcgcga cagtggacaa caaaggcggc atgactgtca
      961 ccgatccgga ttccatcggt attcagatcg acggcgacaa agcggttgtc aataacgacg
     1021 gtgacagtgc catcagtaac ggcggcaccg gcaccagcat caccggcaac gaagccacag
     1081 ccaacaacac cggcaaaacc accgttgacg gcaaagattc caccggcacc gagatcgcag
     1141 gtaataacgc caccgttact caggaaggtg aactcacggt tagcagcggc ggccgcggta
     1201 tcgacatcac aggcaacaat gccaaggtcg acactaaagg caaaatgacc atcacaggta
     1261 ctgactcggt tggtgtgtcc atcaatggcg acagtgcgac gttgaccaat acgggcgata
     1321 ttgacgtcag caattcggct gccggattca gcctcgtaac caatgagggg attatctcgc
     1381 tggctggcag catgaaagtg ggcgattttt caacgggcat ggcgcttagc ggcgacaaca
     1441 acagcgtgac gttggcggcg aaagatatca atgtcaccgg gcagaaagcg acaggcgtta
     1501 acatctcagg cgatagtaat accgttgata tcaccggtaa tattctggtg gataaagacc
     1561 aaaccgcaga taacgcggta gattatttct acgacccatc cgttggtgtg aatatcagcg
     1621 gcaacagtaa cacggtatca ctggacggta agctaaccgt tattgcagat agtgagttaa
     1681 cctcacgcaa atacatggag tttgatggta gccaggagaa tatttctggg cttaccgtgt
     1741 ctggcgatgg taacaccgtt aatttgaatg gcggcattca gtttgtgggc gagaaaaacg
     1801 cgctggcaga cggttcaact attgccgata agcgcagcta ctttggtaag acacctcttg
     1861 ttagtgtcga tggacaatcc aaagtttatc tgaacggtga ttctactatc agcggctctt
     1921 tgccgttagg ctatgccaac attcttcagc taagcaataa ggcggcgctt gaaattggta
     1981 gtgacgctac gttcagtatg cagaacatca gtgtttacga gcactatttc gcgcaaactc
     2041 cgcaaattat caaggttgat acgggttcac aggtcgtcaa caatggtgat gtggatatct
     2101 ggaatatcag ttttgcaggc atttggggcg aaaactctac cggtattaac aacggaaata
     2161 ttacgctttc tcaatatgac tattcttctc cggagacacc gttctcagaa cctgaccaca
     2221 tggccttttt aagctccagt ggcggaagtg ctgtgaacaa cggaacgatc actgcaaaag
     2281 tgatggagca gcacagtgtt ctcgatatgg gagctgcagc cggtgttaca gacccgaggg
     2341 tattcaataa tagcgtgtcg agtatgatgg gaatggaggc ctacggtaaa ggcactgttc
     2401 ttaacagcga aaacggcgtt attgacatgt acgggcgcgg caacattggc atgttggcaa
     2461 ttgataattc cgtggcgaat aatgccggga aaatcacgct ggatacgctt tgggtcgatc
     2521 aaaacgacac cacgacgctg cgtactgacc tcccttccaa cacggctata gattacggtg
     2581 ttggtatggc caccggaacg aatagtggtg gtggtgcaag aagcaacggt gttgcgacta
     2641 accaacaagg cggtgtaatc actgtttata atgccggtgc tgctatggct gcttacggtg
     2701 ccagcaacat ggttattaac cagggcatta tcaatctgga gaaaaacgga aattacgatg
     2761 gcggcttggg cgcgaatatg ctcgtgggga tggcggtcta taacagaggc accgctatca
     2821 acgataaaac aggcgtgatc aatattaacg tcgatacggg gcaggcgttt tataacgacg
     2881 gtacagggac aattctgaac tatggtgaga taaaccttct cggctcgcca atggacagtg
     2941 cagactctca catgggggct attccggaaa atctggatct gctgacagca ctgacaggca
     3001 gcggcgaaac ggatatgcga actgcttcaa gtggcgggtt tgtgacgacc aaagcgctgg
     3061 caaactacgg caatgaaacg ctgaatagca atgtggctgc caaagcgtgg ctttataacc
     3121 aggataaggc caacctgaca attaacggag agctatccat cgggcaaggg ctggaaaaca
     3181 gcggcttact cgattctgac accattagcg cagccgcgaa tgtctataac cgagcatcgg
     3241 gtagcattat cactgatcag ttatcgctaa cgggtagtaa ctcgtttttc aatgagggca
     3301 acttcagcgg ttcggttgct ggctctagct ataagcaaaa tgtggttaac acgggaacga
     3361 tggctgtaat ggccgacggt aagtcgctta tcagtggcag cttcctgctt tataacgaag
     3421 caggcgcaac actcagcaac agcagcagcg ccgtttcggg tggagaaaat gccattgtta
     3481 acgtaacccg caccggcgat tcactcgcgc aagttaaccg tggcaccatt actgcggtca
     3541 acggctacag tgcaattaaa acggcaagta cgggttctaa cagcaacggg aaatggatct
     3601 ggaataccga cacgggggta attagcggcg tgaatccaaa tgcaccgtta attgatcttg
     3661 gtcgcggcta taactttgcc aacgccggca ctattaatgt gcagggtgat ggtgcagtcg
     3721 ctatcagcgg gggaaccacc agctatacgg ttcaactggt gaacagtggg accatcaatg
     3781 tcggtacggc gcagggtaaa gcggacggca ctaacggtac tgggttaatt ggtatcaagg
     3841 ggaatggcag tgatacgaca attaataacg cacaaagcgg tgtaattaac gtttatgcag
     3901 ataattcctg ggccttcggt ggaaagacca aagcgatcat taacaatggt gaaattaacc
     3961 tgctgtgtga tacgggatgt gatatctatg caccgggtac caccggtacg ctgaacgatc
     4021 ataacagtac tactgatatt atcgttcctg cagcaacgag cacacctacg cagggttcag
     4081 tccccacagt acctgcggac tccagtgcac agcaaaaact gactaactac actattggta
     4141 ccaatagcga tggcacctct ggtatgttga aagccaataa cctggtcatc agcgacaacg
     4201 tcaaggtaaa taccggattc agtgcaggta ctgccgatac gactgttgtg attaatgatg
     4261 ttttcaaagg tgaaaatatt tccggggctg agaatatttc ttcttccacg gtaatgtgga
     4321 atgctcaggg cagcactgat gccagcggca acgtagacgt cacgatgacc aaaaacgcct
     4381 atacggatgt tgtgacggat tcttcagtta ataatgtcgc tcaggtactg gattcgggtt
     4441 acaccaataa cgacctgtac actagtctga acgtaggaac gactgctgaa ctgaacagtg
     4501 cgctgaaaca aattagcggc agccaggcaa caacggtatt caatgaagca cgagtgttaa
     4561 gtaaccgttt cagcatgctg tcagatgctg caccggaagt tgcaaacggt ctggccttca
     4621 acgtggtagc gaaaggcgac ccgcgtgctg aactgggaaa taatacacag tacgacatga
     4681 tggcgctgcg taaatccatg acactgactg aacatcagaa tctgagtctg gaatacggta
     4741 ttgctcgtct ggagggtaat ggttcagata ccgtaggcga taatggtgtg actggcggtt
     4801 acagccagtt cttcggtctg aaacaccaga tggcgtttga caacggtatg agctggaata
     4861 acgccctgcg ttatgatgtg cataatctcg atagcagtcg ctcgatagcg tacggcgacg
     4921 tcaacaaaac cgctgatgct aatgtgaaac agcagtatct ggaattccgt agtgaagggg
     4981 caaaaacctt tgaactgcgt gaaggtctga atgtgacacc ttatgcgggt gtgaaactgc
     5041 gtcatacgct ggaaaacggt tatcaggagc gtaatgcggg cgactttaac ctgagcatga
     5101 acagcggcag cgaaacggcg gtggacagca tcgttgggct gaaactggac tatgcaggga
     5161 aagaaggctg gagtgcgaac gccacgctgg aaggtgggcc gaatctgagc tatgtgaaaa
     5221 gtcaacgtac tgcgagtatt tcgggcgctg gaagccaacg ttttaacatt gacgatggtc
     5281 agagtggcgg tggtttcaac agcctggcga cgatgggtgt gaaatacagc agccaggaaa
     5341 gtgcgttgca ggtagatgca ttccactgga aagaagacgg catcagtgat aaaggtgtga
     5401 tgctaaactt caagaaaacg ttctaatttt tagcatgtaa tacataaacc gcaacgctga
     5461 tacaggttgc ggttttttta ttgtcggatg cggtgcttga cgtgtcttaa cctgtctggg
     5521 gtttcgtgtc tttcagggca acagtacaac tcatcccgca atcttgttta caccctcgca
     5581 ttatttatct ctattcattt ctatactgat ttttcttaat ccgttttatt acagggcagg
     5641 gtgcgatgag cagcaacaca tttactctcg gtacaaaatc cgttaaccgt cttggttatg
     5701 gcgcgatgca actggcaggt cctggggttt ttggcccccc aagagatcgc cacgtcgcta
     5761 taaccgtgct gcgcgaggcg ctggcattgg gcgtcaatca tattgatacc agcgactttt
     5821 atggtccgca cgtcaccaat cagattatcc gcgaagcgct ttatccgtac tctgacgacc
     5881 tgacaattgt cactaaaatt ggtgcgcggc gtggagagga cgcatcctgg ttacccgcat
     5941 tttctccggc agagctgcaa aaagcggtac acgataatct acgtaatctc ggcctggacg
     6001 tactggatgt ggttaacctg cgcgttatga tgggggatgg tcatggccca gcggaaggat
     6061 cgattgaagc cagccttacc gtgctggcgg agatgcaaca acaaggcctg gtaaaacata
     6121 ttggcctgag taacgtcaca ccgacgcagg ttgcagaggc gcgaaagatt gccgaaatcg
     6181 tctgcgtgca gaacgaatac aacattgtcc accgagctga tgacgcaatg attgatgctt
     6241 tggctcgtga tggcattgcc tacgtgccgt tcttcccgct cgggggcttt acgccgctgc
     6301 aatcctccac gctttccgat gttgctacaa gccttaatgc gacaccaatg caggtggcgc
     6361 tggcgtggct gttacagcgt tcaccgaata ttttgctgat tcctggcacc tcttcggtgg
     6421 ggcatctgcg ggagaatatg gcgtccgatg aattagtgct aacggatgac gtgttggcaa
     6481 cgctggacag tatttcgcaa aaggcgggtt aaataaaaaa acctcccact ttcgtgggag
     6541 gcatcataaa tcttatgcag caacaatact gtcgatagct gctttcgcgt cagactgcgc
     6601 tttcgctgcc atttcaggac cgtatgcgat cccttcggcg aagacaaatt tcacatcagt
     6661 aatgccgata aagcccagga acgtggacag atacggcgtc accaggtccg ttggtccatc
     6721 tttgtggatc ccaccgcggc tggtaataac gatggctttt ttacccgtta ccagaccttc
     6781 cggaccgttc tcggtatagc ggaaagtaac gcctgcacgt gcaaccaggt caaaatagtt
     6841 tttcaactgg gtcgagatat tgaagttata catcggtgcc gcaataacga taacgtcgtg
     6901 ggctttcagc tcggcaatca gctcatcgga aagcgccaga gcttcctgct gacgcggagt
     6961 cagcggcgca tcgctcggac gcagtgcacc aaccagttcg ccatccagga ccggaatcgg
     7021 atttgtagcc aggtcacgca cggtgatttc atcagcggag tgcttttcgc gccattgttc
     7081 aacaaaataa tcggacaact gattagactg agagtaccct gccaggatgc tggatttaag
     7141 aactaatacc ttgctcatgg tgtttcctta tagatatttg aatgggcaat gccccgttgc
     7201 ttgttgacac tttattcaca atcctgccac agagatagcg caataaatcg aagcctatgt
     7261 tcgaatttat tgaacaacgc atagaaagcc gcgatgtggt actctatacc tatcatttaa
     7321 aagaaaatta atcaggcaga ctactgccca ctaacgttat gacagaacaa caaaaattga
     7381 cctttactgc cttgcagcag cggctggatt cgctgatgct gcgtgacaga ctgcgttttt
     7441 ctcgccgtct gcacggcgtg aagaaggtta aaaatcctga tgcacaacag gccattttcc
     7501 aggagatggc gaaagagatt gaccatgcgg cagggaaagt cctgctgcgt gaagcggcac
     7561 gaccggagat tacttacccg gacaatttgc cggttagtca gaaaaagcag gacattctcg
     7621 aagcgattcg tgaccaccag gtggtgatcg tcgccgggga aacgggttcc ggtaaaacga
     7681 ctcagctacc gaaaatctgt atggagctgg gtcgcgggat taaagggctg atcggccata
     7741 cccagccgcg tcggctggcg gcgcgaacgg tggcgaaccg tattgcggaa gagctaaaaa
     7801 cggagccggg cggttgcatc ggttacaaag tgcgtttcag cgatcacgtc agtgataaca
     7861 cgatggtcaa gctgatgacc gacggtatcc tgctggcaga gatccagcaa gaccgcctgc
     7921 tgatgcagta cgacaccatc attattgacg aagcgcacga acgcagcctg aatatcgatt
     7981 ttctcctcgg ctatttgaaa gagttgctgc cgcggcgtcc tgacctaaaa atcattatca
     8041 cttccgcgac tatcgacccg gaacgctttt cgcgccactt taataatgcg ccgattatcg
     8101 aagtctccgg tcggacctat ccggtggaag tgcgctatcg cccgattgtt gaagaagccg
     8161 atgataccga gcgcgatcag ttgcaggcga tttttgacgc cgtagatgaa ctgagccagg
     8221 aaagccctgg cgacattctg atctttatga gcggcgagcg agaaattcgc gataccgccg
     8281 atgcgctgaa caagctgaac ttacgccata ccgaaatctt gccgctttat gcgcggcttt
     8341 ccaacagcga acagaaccgc gtgttccagt cgcacagcgg gcgacgcatt gtactggcga
     8401 ccaacgtcgc ggaaacctcg ctgaccgtac cgggtattaa atacgttatc gacccaggta
     8461 cagcgcgtat cagccgctac agctatcgca ccaaagtgca gcgtttgccg attgagccaa
     8521 tttcgcaggc ttcagctaat cagcgtaaag gccgctgcgg ccgtgtgtca gaagggatct
     8581 gtattcgtct ctattccgaa gacgatttcc tctcgcgccc ggagtttacc gacccagaaa
     8641 ttctgcgtac taacctggcc tcggttattt tgcagatgac cgcactgggc ttgggcgata
     8701 tcgccgcatt cccgtttgtc gaagcaccgg ataaacgcaa tatccaggat ggcgtgcgtc
     8761 tgctcgaaga gctgggcgcg atcaccactg atgaacaggc cagcgcctat aaactgacgc
     8821 cgctcggtcg ccaactctcg cagttgcctg tcgacccacg tctggcgcgt atggtgctgg
     8881 aagcgcagaa acatggctgc gtgcgtgagg cgatgattat cacttccgcg ctctctattc
     8941 aggacccgcg tgagcgtccg atggataaac agcaggcatc tgacgaaaaa catcgtcgct
     9001 tccacgacaa agagtctgac ttcctggcat ttgtgaatct gtggaattat cttggcgagc
     9061 agcaaaaggc gctttcttca aacgccttcc gtcgcctgtg tcgtactgat tatctcaact
     9121 atctgcgcgt gcgcgaatgg caggatatct acacccagtt gcgccaggtg gtgaaagaac
     9181 ttggcattcc ggttaacagc gaaccggcgg attatcgcga aattcacatt gcgttgctga
     9241 ccggtttgct ttcgcatatc ggcatgaaag atgccgataa acaagaatat accggcgcac
     9301 gtaacgcgcg tttctccatc ttccccggtt ctggtttatt caaaaagccg ccgaaatggg
     9361 taatggtggc ggaactggta gaaaccagcc gcctgtgggg gcgcattgcg gcacgcatcg
     9421 atccagaatg ggtggaacct gtcgctcagc atttaatcaa acgcacctac agcgaaccgc
     9481 actgggaacg ggcgcagggc gcggtgatgg caacggaaaa agtcaccgtt tacgggttgc
     9541 cgattgttgc tgcgcgtaag gtcaactaca gccagatcga tccggcgtta tgtcgtgaac
     9601 tctttattcg ccacgcgctg gtggaaggtg actggcagac gcgtcacgca ttcttccgtg
     9661 aaaacctgaa actacgggcg gaagtagaag agctggaaca caaatcacgt cgccgcgata
     9721 ttctggttga tgacgaaacg ctgtttgagt tctacgacca gcgcatcagc cacgatgtga
     9781 tttccgcacg tcacttcgac agctggtgga aaaaagtcag ccgcgaaacg cctgatttgc
     9841 tcaactttga aaagagcatg ttgatcaaag aaggggcgga aaaaatcagc aagctggatt
     9901 acccgaactt ctggcatcag ggcaatctca agctgcgttt gagctatcag tttgagcccg
     9961 gcgcggatgc tgacggtgtg accgtgcata tcccgctgcc gctacttaac caggttgagg
    10021 agagtgggtt tgagtggcag atccccggtc tgcgtcgtga gctgatcatc gccctgatta
    10081 aatcgctgcc gaagccggta cgccgtaatt ttgtgcccgc gccaaactac gccgaagcgt
    10141 ttttaggccg cgtcacaccg ctggaattgc cgttgctcga cagccttgaa cgagagttac
    10201 ggcggatgac tggcgtcacc gttgaccgcg aagactggca ctgggatcag gtgcccgatc
    10261 acctgaaaat cactttccgc gtggtggatg acaaaaacaa gaagctaaaa gaagggcgct
    10321 cattacagga tctgaaagac gccctgaaag gcaaagtgca ggaaacgctg tctgccgtgg
    10381 cggatgacgg tatcgagcag agcgggctgc atatctggag ttttggtcaa cttccggaaa
    10441 gttacgagca gaagcgcggc aactacaaag tgaaggcgtg gccagcgctg gtggatgagc
    10501 gtgacagcgt agcgatcaaa ctgtttgata acccgctgga acagaagcag gcaatgtgga
    10561 acggtcttcg ccgtttactg ctgctaaata ttccatcgcc gatcaagtac ttgcacgaaa
    10621 agctgccaaa caaagccaag ctggggctgt actttaaccc gtatggcaaa gtgctggagc
    10681 tgatcgatga ctgtatctcc tgcggtgtgg atcaactgat cgacgccaat ggtggcccgg
    10741 tctggacgga agaagacttt gctgcgctgc atgaaaaagt ccgcgctgaa ctgaacgaca
    10801 cggtggtgga tattgcgaag caggtcgagc aaatcctcac tgcggtgttc aatatcaaca
    10861 aacgtctgaa agggcgggtg gatatgacca tggcgctggg gctttctgac attaaagcgc
    10921 agatgggcgg gctggtgtat cgcggttttg tcactggtaa tggcttcaaa cgactgggcg
    10981 acacgctgcg ttatttgcag gcgattgaaa aacgactgga aaaactggcg attgatccac
    11041 atcgcgaccg tgctcagatg ctgaaagtcg aaaacgtcca gcaggcgtgg cagcaatgga
    11101 tcaacaaact gccgcctgca cgtcgtgagg atgaagacgt gaaagaggtc cgctggatga
    11161 tagaagagct gcgcgttagt tacttcgctc aacaacttgg tacgccttat ccgatttcgg
    11221 ataagcgtat tttgcaggcg atggagcaga ttagcggtta acactgctat ttgcctgata
    11281 aatctaaaac ccggtaagca tttagcgccg ggttttttat taattctaaa aacgataagg
    11341 gaaaaaattc aggagttcag aaaaatacaa t
//
LOCUS       DAZFJL010000143        11344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFJL010000143 DAZFJL010000000
VERSION     DAZFJL010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.145"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..1471)
                     /locus_tag="ACHJ8C_003890"
     CDS             complement(<1..1471)
                     /locus_tag="ACHJ8C_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5553673.1"
                     /translation="MSVLSWAIDLCRKTEAWLVKRQAKDQLQCVEQGLSLTEGEHGER
                     YDTLAPKTINDGSMREYFIALKYALSQKDVRNIAITGSYGAGKSTVISSFMKYHCDDK
                     YINVSLAGFDMTENETTVSPTHQEVELSILQQILYKENREKLPDSRIDRIIDRGPHYV
                     RGTYRALLKVIVPIAVAAFIIYFKTIAEFLSLPPSWTEVFNAHYIIKGIILVLLAFTA
                     LYFITASASRIGIFDKKLKLSKIALLSGDVEASEQETPSLLNNCLDEIVYFFTRLEYK
                     VVIFEDLDRLGTPEIFVKLREINKIVNNNIVDGNPVRFVYAVRDDLFLGPDARTKFFD
                     FILPVIPFMDSRNAFTLLKRKMSLDTHNDKYLKDISAYINDMRSLQNLVNEYHVFSNI
                     VDNGKDKIKLLSLVFYKNIYALDYFLTDKKTGVLYSFIRDYRTRKLHRNHFDSLETKL
                     DLLQAEIEHLTHEPMYDHRGIRENIVCRYIPQVLWNKVFF"
     gene            complement(1985..3382)
                     /locus_tag="ACHJ8C_003891"
     CDS             complement(1985..3382)
                     /locus_tag="ACHJ8C_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553674.1"
                     /translation="MSTEKDYPVKIPKWVYDRITEITDCVVGDTQWAVTRRQTLRWFL
                     AHIWLEADDDGWTICTVRDIRSCYASLLSECEINYQGRASISMLVDFLPTLPDIEFRA
                     GKASKDPDKRKANAWLFNPLRPVCDESARGKLNLVDADTGESVSFRALLQGNGKAPGH
                     AIDVEKRQTALKQTEKDFLVKVGRGRMSIQFVKELRSREPDHYYRAGIRSLNHLYNGR
                     IEGQYVTYDHHYRLTFGGRYYDQAFQNLPNEFKAKFRTGLLNYDIEACNLACLNHLFR
                     QYDVDYRVKSSIYKTMMEHTGLTRKQCKQMVHTTTYRIGRVTIGVNDGLGAKVYQWCG
                     NSQKKALKILRWWNRYISPLKSALESLLERVHGTHRKSCSSPRNYHRYANEVGLILDL
                     NSEVYEREKTHHQQYARDKALLAFMICGVEQSYIREVVRLNPGRVCMLDHDGVVATGA
                     LSLPDWRGFTMKVKD"
     gene            complement(3909..4862)
                     /locus_tag="ACHJ8C_003892"
     CDS             complement(3909..4862)
                     /locus_tag="ACHJ8C_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553675.1"
                     /translation="MNESYYFRTSDPDYIRHAPSRLEILSKAFPTIITQQESEHELQR
                     LIHLLNKLKGSNRCYNIIAQKLRQCRNGSPCNSLICPHCQRERILAQLAMLHVLPGNS
                     AEYVGVVLFFNKDTQTPPPWKNTDALRAQIGRYKQRISRVLNRLGYAGPATGTFSMMR
                     HMPDGPEARIFWVPQLCLFLPNDSTLIKGLKAHMSRSSGEFIDATTVNTPVIVPRYKD
                     PARLISCALDPAWHTTDYTLTDKDALVKSRVAPLKGRTLLKSLLALDSLGTGAVSFSF
                     GQPQGKCIDFPVMQPLQCTFVVDQIAVERRVTLATTAFSLL"
     gene            complement(4958..5416)
                     /locus_tag="ACHJ8C_003893"
     CDS             complement(4958..5416)
                     /locus_tag="ACHJ8C_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553676.1"
                     /translation="MKEIKSNTSAANKKLIPWFKTMGKQIGLSQSLIAAVLKEQRIRI
                     HVLNGASAYWVSIALTQDFGESDGLWNAYGKLALILERCKYIPCGSRACAYCTAAQQL
                     HYQGVDIGGKRSLYQEPAVCLDFLNPNDPNSSYYWDAKNLVERHGDGMLK"
     gene            complement(5418..5615)
                     /locus_tag="ACHJ8C_003894"
     CDS             complement(5418..5615)
                     /locus_tag="ACHJ8C_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5553677.1"
                     /translation="MNTNRILRKKEVLHLTGISSATLYRLISKGVFPLSKKLTGDSGR
                     AVGWLESDINNWVNSRMQAGE"
     gene            5839..6453
                     /locus_tag="ACHJ8C_003895"
     CDS             5839..6453
                     /locus_tag="ACHJ8C_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553678.1"
                     /translation="MIGNQSNKADARCHRERKSSNEVFEFLEKKLYLGRDTRQKLALI
                     ARKLTGAGFSPDKFAAEKAADVLSACVNHMFNDLLNEGELNGIYRSEEHPMIVPARSP
                     KALEIYTVYQLVKGRFDKLHTGESDREKYASVAEKLNGYGIWKPRLKRLSDGKEWARE
                     DVAWVLQPENINALINVQNQKYARLSAEDKTKAASGNGSKAKNT"
     gene            complement(6440..7369)
                     /locus_tag="ACHJ8C_003896"
     CDS             complement(6440..7369)
                     /locus_tag="ACHJ8C_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019083242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5553679.1"
                     /translation="MSRAALRQAVPVVQTTVFDAYINGLAHSGRRGITSLLNRSAGIL
                     KRGADAAGYPWEQLNYAAVAKVRAALLDDGYAVSSVNMALSALRGVAQTAFNLNCMNA
                     ETLARIRSVKRVSGDIQRKGRALDRQEIRALIHAAKQHPQHVRRYRDVAIALTLCGTG
                     LRAGELVKLERRDYDNGVLTVRQGKGRKYREIHVADAVDKAIRAWLKVGADEADSALF
                     SRIQRNGKVASQPLTTTGLTGILADLQQTSGIARFTPHDMRRTFITRLLEQGVDINTV
                     RQLAGHSDISTTTRYDCRGESMKISASKRIKCF"
     gene            complement(7416..7868)
                     /gene="radC"
                     /locus_tag="ACHJ8C_003897"
     CDS             complement(7416..7868)
                     /gene="radC"
                     /locus_tag="ACHJ8C_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO5553680.1"
                     /translation="MTLFTQRETRILDQARDIISRYYQRGVQLCSPDDVRRCVMVELA
                     PLEHEEFGIILLDNQNQLLHREILFRGTLNSVSVHPREVIKRVLKHNAAAVILVHNHP
                     SGEPEPRRCDIKLTKKLQELLEMLDVRLLDHFIVAGTETVSMAERGLM"
     gene            complement(7870..8331)
                     /locus_tag="ACHJ8C_003898"
     CDS             complement(7870..8331)
                     /locus_tag="ACHJ8C_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553681.1"
                     /translation="MLSWLYDGRVKRRPLMNRLIRAYQQRWPLHEWLTEGIEEDRLDW
                     LIAQVLQKGHYGRQFPVQITRPFAGKRGLSDGRLFREMQRFLDVTDHSRLIMLSDQFH
                     WSLITKMDEETLCFFDSNGRTTMPRKAFSLRTGVTRRQLFPDAIYFIEREF"
     gene            complement(8451..8843)
                     /locus_tag="ACHJ8C_003899"
     CDS             complement(8451..8843)
                     /locus_tag="ACHJ8C_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2787 family protein"
                     /protein_id="HFO5553682.1"
                     /translation="MPVDQELINIILNEAGNPPPHKTKITAVSLLFKDLSYSAEKGGY
                     HPVEIRLISRNDEWYFDYITDFGYMGTVYPELEKEIDISWSQQYVFLAYRGDLPVNAG
                     RELFELWQSNFIQYHAMNAYTITVLWES"
     gene            complement(9197..9685)
                     /locus_tag="ACHJ8C_003900"
     CDS             complement(9197..9685)
                     /locus_tag="ACHJ8C_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553683.1"
                     /translation="MSSKRSVSAIPTHPEASVEEAKDWLTAEEFTHIEGMPGTARGAR
                     MRLEKLASLHPEIRRKRTAGKGVVYHISAATMSLDDERNRAKGEAFTLTDSDRQLSLW
                     IQLYRNMSPASRTQLMEQALRLVKDDLMSQGPDVSELAVQIMKLPEDERKCLFEKLNG
                     KV"
     gene            complement(9799..9951)
                     /locus_tag="ACHJ8C_003901"
     CDS             complement(9799..9951)
                     /locus_tag="ACHJ8C_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006324787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553684.1"
                     /translation="MYVMQGRDCVTVTPALTLACEVLPQYRHDASSLNFLCFAFSAML
                     LSYAQY"
     gene            9879..10076
                     /locus_tag="ACHJ8C_003902"
     CDS             9879..10076
                     /locus_tag="ACHJ8C_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5553685.1"
                     /translation="MAALHKRGLTLASLSRSHGLASRTLNNALERHYPRAERIIAGAL
                     DTTPETLWPERYALKNGPNSR"
     gene            10088..10615
                     /locus_tag="ACHJ8C_003903"
     CDS             10088..10615
                     /locus_tag="ACHJ8C_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553686.1"
                     /translation="MATTITEITVCFEYFFSSLYRREFVKTLRLNECSERELLPLVRC
                     YLLGWFADNVSPEVKSKLPGTVSGHGYIDFVIDDVAVEFAVRKPTAARSNVSATVNST
                     EVKKLMKHDGKALLVLFDFSDTPYSEEQIESFRNWPSLGRGNHRKSAFNVVYFFVEKR
                     RPLGLGKITKNIRIT"
     gene            10630..10989
                     /locus_tag="ACHJ8C_003904"
     CDS             10630..10989
                     /locus_tag="ACHJ8C_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO5553687.1"
                     /translation="MNRKLLALLMVAVTLPVAAAQYVKPGVVLTLNPAKGNAEFNINA
                     SMGDGSGVCNMEGTAQQIAAGENQKRRWVWNDNTSQCVAVISELRNGKTSIMTRGCEG
                     YCGASAAAAMDGLFNKK"
     gene            11020..>11344
                     /locus_tag="ACHJ8C_003905"
     CDS             11020..>11344
                     /locus_tag="ACHJ8C_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553688.1"
                     /translation="MKGTMFLVLIAAGLLAGCGEKTPKCSSDDAKNLVVDIARKTIEK
                     GMTLDKDVRITVENVRTISHESGLDIYQCAADLTFTKPDLQNSLPITYRIQKTDEGKG
                     QFYINV"
BASE COUNT         2972 a         2801 c         2596 g         2975 t
ORIGIN      
        1 caaaaaaaac cttattccaa agtacttgcg gaatatatct acaaactata ttctctctga
       61 tccccctatg gtcatacata ggctcatgag taagatgttc tatttcagcc tgtaaaagat
      121 ccagttttgt ttccagagaa tcgaaatgat tacggtgaag tttgcgggtg cgatagtcgc
      181 ggataaatga gtataaaact ccagttttct tatcggtaag aaaatagtct aaagcataga
      241 tatttttata aaacaccagc gataacagtt tgattttatc tttgccgtta tcaacgatat
      301 tgctgaatac atggtactca ttgaccaaat tctgcaggct gcgcatatca tttatatatg
      361 ccgaaatatc tttcaaatat ttatcgttat gcgtatcgag cgacattttc ctctttaata
      421 aggtaaacgc gtttctgctg tccataaatg gaatgacggg aaggatgaaa tcaaagaatt
      481 tagttctggc atcagggccc aagaataagt cgtctcttac cgcataaaca aacctgaccg
      541 gattgccatc aacaatatta ttgttaacta ttttgttaat ttcacgcagc ttaacgaaaa
      601 tctcaggcgt tcctagcctg tcaagatctt caaagataac caccttatat tccagcctgg
      661 taaaaaaata cacgatctcg tcaaggcagt tattcaataa ggatggcgtc tcttgctcgc
      721 ttgcctcaac atccccgctt aaaagggcga tcttgctcag cttaagtttc ttatcaaaaa
      781 taccgattcg agatgcgctg gcagttataa agtacagtgc cgtaaaggcc agtaaaacta
      841 atataatgcc ttttataata tagtgcgcat taaacacctc ggtccaggac ggcggaaggc
      901 tcagaaactc tgcaattgtt ttgaaataga tgataaatgc tgcaaccgcg atgggcacaa
      961 tgactttaag taaggcccga taggttcccc tgacatagtg agggcccctg tcgattattc
     1021 tgtctatcct tgagtcagga agcttctctc tattctcttt ataaagaatt tgttgcagga
     1081 tactgagttc aacctcctgg tgagttgggc taacggttgt ttcattttcc gtcatatcga
     1141 atcccgcaag agatacattg atgtatttat catcacagtg atatttcata aatgatgaaa
     1201 taaccgtact tttccctgcg ccgtagctcc ctgtgatggc aatgtttctc acgtctttct
     1261 gagacaaagc gtatttcagg gcaatgaaat actctcgcat actgccatca ttgatagttt
     1321 ttggcgctaa ggtgtcgtag cgctcaccgt gctctccttc cgtcaaactc agcccctgct
     1381 ctacgcactg tagctgatct tttgcctggc gcttaaccag ccacgcttcc gtcttcctgc
     1441 acaggtctat tgcccagctc aacacgctca ttcagcgact ccattttgcc aatacccgat
     1501 tacgataagc ggaaattctc aactgcgttt agtgacgtcc agaataatct gaacgggtat
     1561 atcgtagcgt ttcaacacga tgaaaacatg ttcttttttg agttactcgc gcggattcac
     1621 atttggcatg tttgagaagg tatctctggg gatcatgaca cagcctcagc tctgtgtacc
     1681 aagtaacatg catggtgcgc tcctaagtcg aagctcttgt ataggctgct ggcaacatgc
     1741 tgtagcttat ccacaatttg atattgtccg gtccagcatt aacgctccgc tacatacggt
     1801 caggctgcaa ctatttgcat cttggataac gaactatgca ctttaagagg tatcagcaag
     1861 catgacttcc gctcttcact catagcggaa atggatcact tgtcggatat gcatatccca
     1921 ttatgattga caacgtaata cgcccttatc agagatcgca gatagttaaa ttcaagaaag
     1981 caagctagtc cttcaccttc atcgtgaaac cccgccagtc cggcagtgaa agcgcgcccg
     2041 ttgccactac cccatcatga tccagcatac agacccgccc tgggttaagt ctaaccacct
     2101 cacggatgta cgactgctcc acaccacaga tcataaacgc caagagtgcc ttatcccgtg
     2161 catattgctg atggtgagtc ttttcacgct catacacctc actattcaga tccagtatta
     2221 atccgacttc attagcgtac ctgtgatagt tccgcggtga tgaacaactc ttgcggtgtg
     2281 tgccatgcac acgttccagc aatgactcaa gcgcagattt caatggactg atataacggt
     2341 tccaccaacg caaaatcttt agcgcctttt tttggctgtt accgcaccac tgatacactt
     2401 ttgccccaag gccatcatta acaccaatag tcacacgccc aattctgtag gtggtggtat
     2461 ggaccatctg tttgcactgt ttacgggtca gcccggtatg ttccatcatg gttttatata
     2521 tcgatgactt cacacgataa tcgacgtcat actgtctgaa cagatggttc agacaggcca
     2581 gattgcacgc ttcgatgtca tagttgagta acccggtccg aaatttagct ttgaactcat
     2641 tgggcagatt ctggaaagct tgatcataat accgccctcc aaacgtcaga cgataatgat
     2701 ggtcgtaagt gacatattgt ccctcaattc tgccattgta gagatggtta aggctgcgta
     2761 tacctgcgcg gtaatagtga tccggctcac ggctacgtaa ttccttcaca aactgtatac
     2821 tcatgcgccc ccgcccaact ttgaccaaga agtccttttc cgtttgtttt agggccgtct
     2881 ggcgtttctc tacatctatc gcatgcccag gagctttccc gttcccctga agcagagctc
     2941 taaaactaac agactcacct gtatccgcat caaccagatt taacttaccc ctggcagact
     3001 catcacacac aggccgcagg ggattgaaca gccatgcatt ggctttacgc ttgtccgggt
     3061 ctttactcgc tttccctgca cgaaattcaa tatccggcaa tgtcggcagg aaatcgacta
     3121 gcatggatat ggaggccctg ccctggtaat tgatttcaca ttcacttaga aggctggcgt
     3181 aacaactacg gatatcccgg acagtgcata tagtccaccc atcatcgtct gcctccagcc
     3241 atatgtgggc caggaaccac cgtaatgtct gccgtcgggt gacagcccat tgtgtatccc
     3301 ccacgacaca atccgttatt tcagtaatac ggtcataaac ccacttcggg atcttcactg
     3361 gataatcctt ttcagtactc acggccccgt ctcagcatgg tgttgttgca tcagctatta
     3421 cgttgactct tacgcagacc atttcaccag tttttaagtc gggtaatttg aaagtcaaaa
     3481 ccccagtcgc gaggatgggc tccgcccatg atttgctcca ctccggtcgc aaatctttcc
     3541 ccctgttcag gctgacgcct tccctcactc gctgcgctcg ttcgccatgt tgtttcatga
     3601 ctggagaagc ggataaagcc tccgttatgc tgcgtgtccc caggctctcc gttgcgctca
     3661 atggcaatgg tgctcgctga ccgctccccc cattgtgtgt agaatattca ctattgtgtg
     3721 tagaatattc actggcagaa acgcagaaag tcctcgcaga tgcgctaacc cgtcagttga
     3781 tggcaaggat cgggtgcaca gaggcatcag taagactctg cagaaaacag acagttacgc
     3841 taaaaccgga acagttggaa ttttaaagtg catcagtgcc agcagaatca caggcgagcg
     3901 ctgcagtttt acaacaacga aaatgcagtg gttgccagtg ttacccgtcg ttccactgca
     3961 atttgatcaa caacaaaagt gcattgcagt ggctgcatta ccgggaaatc aatgcacttt
     4021 ccctggggtt gcccaaacga aaaagaaacg gcacctgttc ccagtgagtc cagggccagt
     4081 aaacttttta acagtgtccg acctttcaat ggcgctactc tggacttaac aagagcgtct
     4141 ttatcagtca gtgtataatc cgttgtgtgc caggcgggat ccagggcgca tgaaattagc
     4201 cttgcggggt ctttatacct gggcacgatc accggcgtgt taactgtcgt cgcatcaata
     4261 aattccccac tacttcgcga catatgcgct tttagcccct taatcaacgt actgtcattg
     4321 ggcaggaaca aacacaactg tggaacccag aaaatacgcg cttctgggcc atcaggcata
     4381 tggcgcatca tactaaaggt gcctgtggcc ggaccggcgt accccagtcg gttaagtacc
     4441 cggcttatcc tctgcttata acgaccaatt tgcgccctga gcgcatcggt gttcttccat
     4501 ggcggcggag tttgcgtatc cttgttgaaa aacaggacca ctcccacata ttctgctgaa
     4561 ttccccggca ggacatgcag catagccagt tgcgccagaa tgcgttcccg ctggcaatgc
     4621 gggcatataa gactgttgca tggagatccg tttctgcact gcctgagttt ttgagcaatg
     4681 atgttgtagc agcgattgct gcctttcagt ttatttagta aatgaataag acgctgcaat
     4741 tcatgctcac tttcctgctg cgtaataata gtggggaatg ctttacttaa aatctcaagc
     4801 cttgacggtg cgtggcgaat gtaatccggg tctgatgttc tgaagtaata agattcgttc
     4861 atttaatatt cctggctgta ttgattaccc tgccaccaaa attcagtggc agggtaatat
     4921 gtcagtcttt caccatgcag aaatggctat ctaccgatta cttcagcatg ccatccccat
     4981 gcctttcgac taaatttttt gcatcccagt aataggaaga attagggtcg tttgggttta
     5041 aaaaatctaa acacactgca ggctcctgat ataaactacg cttcccgccg atatccaccc
     5101 cctgataatg caattgctga gcggcagtac aatatgcaca cgccctgctc ccgcatggaa
     5161 tgtatttgca tcgttcaaga ataagtgcca gtttaccgta ggcattccac aggccatcgc
     5221 tctctccaaa atcctgcgtg agggcaatgg aaacccagta agcagatgca ccgttaagca
     5281 catgtatccg gatccgctgc tcctttaaca cagcagctat cagagattgt gataaaccaa
     5341 tttgtttccc catggtttta aaccagggaa ttaacttttt gttcgcagca ctggtgttcg
     5401 attttatctc tttcatttta ctctcccgcc tgcatacggc tgttaaccca gttattgata
     5461 tcactctcca gccagcccac cgcccggcca ctgtcgcccg ttagcttctt tgacaaagga
     5521 aaaactcctt tcgagatgag ccggtacaat gtcgctgacg agatccccgt cagatgaaga
     5581 acctcttttt tgcgcaaaat acggttggtg ttcatgcttg tgctcctttg agctgtttca
     5641 ggagcggggt tatttacaac cactaaaaaa cgcaaggatt cactcaacga tgccttcgtt
     5701 gagcacttta agggcatctt tgatgtggat gtcattcgag aacacctatt tctagcacca
     5761 aaaagatcac atcaatggct attggccact gaggctcaat gatgatattt tgaacacaac
     5821 actctcatgg aggtgactat gattggtaac caaagcaaca aggctgacgc cagatgccac
     5881 cgggaacgga aaagcagtaa cgaggtcttt gaatttctgg agaagaagct ctatctgggg
     5941 cgggataccc ggcaaaagct ggcactgatt gcccggaaac tgacgggagc gggttttagc
     6001 ccggataaat tcgctgcaga gaaagcggcg gatgtgctga gtgcctgcgt gaaccacatg
     6061 tttaatgacc tgctcaatga gggagagcta aacgggattt ataggtcgga ggaacacccc
     6121 atgatagtcc cggccaggtc ccctaaggca ctggaaatct acactgttta ccagcttgta
     6181 aaaggtcgtt ttgacaagct tcatacgggg gagtcagatc gtgagaaata tgcttcggta
     6241 gcagaaaaac ttaatgggta tggaatatgg aaaccccggt taaaacgact ctctgacggt
     6301 aaagaatggg cgcgtgagga tgttgcgtgg gtacttcagc ctgaaaatat caatgcactg
     6361 attaacgtcc agaatcaaaa gtacgcgcgg ttgagtgctg aagataaaac taaagccgcg
     6421 tctggaaacg gctcaaaagc taaaaacact tgatgcgttt gctggcgctg attttcatgc
     6481 tctcaccacg acagtcatag cgggttgtcg tggatatgtc gctgtggcct gccagttggc
     6541 ggacggtgtt gatatccaca ccctgctcca gtaaacgggt gatgaaggtg cgccgcatgt
     6601 catgtggggt gaaacgtgca atacctgacg tctgctgtaa atcagccagg atacccgtta
     6661 acccggtcgt agtcagcggc tggcttgcga ccttaccgtt gcgctgaatt cggctaaaca
     6721 acgcactgtc agcctcgtct gcgccaactt tcagccacgc gcgaatagcc ttatccactg
     6781 catccgccac gtgaatttca cggtatttac gcccctttcc ctgtctgacc gtgagaacac
     6841 cattgtcata gtcccggcgc tcaagtttaa ccagttcccc ggcccgcagg cctgttccgc
     6901 aaagtgtcag cgcaatagcg acatcacggt agcgccgtac atgctgcgga tgctgttttg
     6961 cggcatgaat cagcgcgcgg atttcctgcc tgtccagcgc ccttcccttg cgctgaatat
     7021 cgccgctcac gcgcttaact gaccggatac gtgccagcgt ttcggcattc atacaattga
     7081 ggttaaaggc cgtctgcgcc acaccccgga gtgctgacag cgccatgttg accgatgaca
     7141 cggcgtaacc gtcatccagc agtgccgcac gtactttggc aacggcggcg tagttaagtt
     7201 gttcccatgg ataacctgct gcatccgcac cgcgcttaag tataccagcg ctgcggttta
     7261 acaggctggt gatgccccgt cgccccgaat gagcaagacc gttgatatac gcatcaaaca
     7321 cggtggtttg caccaccggc acggcctgac gtaacgcagc cctgctcatg ggcgtaaccc
     7381 gcaataacgt acattaaacc aggtaacttt cagtatcaca tcagcccccg ttcagccatc
     7441 gacaccgttt cagttcctgc cacaataaag tgatcaagta accgcacatc cagcatttcc
     7501 agcagctcct gaagcttttt agtcaacttg atatcgcagc gcctgggttc aggctcgccg
     7561 ctggggtggt tgtgaacaag gatgactgcg gcggcgttat gtttcagtac acgcttgata
     7621 acttcacggg ggtgaacact gaccgagttt aacgtccccc tgaacaggat ttcgcggtgc
     7681 agcagctgat tctgattatc gagcaggatg atgccaaact cttcatgttc aagcggtgcc
     7741 agctcaacca tcacgcaccg tcgaacatca tcaggggagc agagttgaac cccacgctga
     7801 tagtaccggc tgataatgtc acgcgcttgg tcaaggatac gggtttcacg ctgggtaaat
     7861 agggtcatgt cagaactccc gttcaatgaa gtaaatggcg tcgggaaaca gctggcggcg
     7921 ggttacgcca gtacgcaggg agaaagcttt acggggcatg gtggttcgcc cgttactgtc
     7981 gaagaagcag agcgtttctt cgtccatttt ggtgataagg gaccagtgaa actggtcgct
     8041 gagcataatc aggcgggagt ggtcagtgac gtccagaaac ctctgcattt ccctgaaaag
     8101 ccgcccgtct gataatcccc gcttcccggc aaagggtctg gtgatttgaa ccgggaactg
     8161 gcggccgtag tgccctttct gcaacacctg cgctatcagc cagtccagac ggtcctcttc
     8221 aatcccttca gtcagccatt catgcaaagg ccagcgctgc tgataagccc gaataagccg
     8281 gttcattaac gggcgtcgtt tcaccctgcc gtcatacagc cagctcagca tgttaaccac
     8341 gcaataagca ccgcaaagac agtccagttc accctgttga gacgcacgta tccctctgtt
     8401 atcagtcaca gataattccc cataaggtag tgatgattag tgcggcggga ttagctttcc
     8461 cacaggacag taatggtgta agcgttcatg gcgtgatact gaataaagtt ggattgccag
     8521 agttcaaaca actcccttcc ggcgttaaca ggcaggtccc ccctgtacgc cagaaagaca
     8581 tattgctgac tccagctaat atcaatttcc ttttccagtt cggggtatac cgtccccata
     8641 taaccgaagt cggtgatgta atcaaaatac cattcatcat tgcgtgaaat aagtcgtatt
     8701 tcgacggggt ggtaaccacc cttttcagcg ctgtaactca ggtctttaaa taacagactg
     8761 acggctgtaa ttttggtttt atgcggcggt ggatttcccg cttcatttaa tatgatattt
     8821 attaattctt gatcgacggg catagctagc ccttcctgag ggacaatatt catcagtgat
     8881 tttcctgttc aacatattct gaaaagacgt tatgtctgca ccagcacagg tggggtttaa
     8941 tcacctaata aatggtaagt gcatggcaat gtactgactg ggtaaaatat agttcattag
     9001 caatattatt atatcaaaaa tatactttaa tgcaaatagt aacaatgaga aaccaatatg
     9061 tagttcagaa atgaaataac tgacagtagg tttttattca ttattaagtg atggcatact
     9121 ttccattctg ccatgtcaga caggtcagga ttccggggtg gttccttctg tctggcatgg
     9181 cggaaaactg acgctatcac accttcccgt tcagcttttc aaagaggcac ttacgctcat
     9241 cttcaggcag cttcatgatt tgtaccgcca gttcagaaac atcaggcccc tgagacatca
     9301 gatcgtcctt taccagcctg agggcctgct ccatcagttg cgtccgcgaa gctggtgaca
     9361 tattgcgata caactgtatc cagaggctaa gctggcgatc gctgtcggtg agagtaaacg
     9421 cttcaccctt agccctgttt ctctcatcat caagcgacat tgtggctgcg ctgatatggt
     9481 aaaccacgcc tttcccggca gtccttttac gacgtatttc cgggtgcagg gatgccagtt
     9541 tttccaggcg catcctagcg ccacgcgcag tgcccggcat cccctcaata tgagtgaatt
     9601 cctctgctgt cagccagtct ttagcctctt caacagaagc ttcaggatgg gtcggtatag
     9661 ccgatactga tctcttactg ctcatataaa tttcaccaga tatcaatgcg ttaatatgaa
     9721 aaccaacaaa tagataccaa aagaacttga aaggtatctt ttattcgcat ttaatatcca
     9781 tgtactgatt ccatggagtt aatactgtgc gtaactcaac agcatcgcag aaaaagcaaa
     9841 gcaaaggaag ttcagggaac tggcatcgtg ccgatattgt ggcagcactt cacaagcgag
     9901 ggttaacgct ggcgtcactg tcacgcagtc acggccttgc atcacgtaca ttaaataacg
     9961 ccttagagcg tcattatccc agagctgagc gcattattgc cggggctctg gatacgacac
    10021 cggaaacatt atggccggaa aggtatgccc ttaaaaatgg acctaatagc aggtaaagga
    10081 tgcaaccatg gcaaccacca tcactgaaat aacggtatgc tttgagtatt ttttcagttc
    10141 tctttatcgc agggagttcg tcaaaaccct gcgtctgaac gaatgtagcg agcgggaatt
    10201 actgcctctg gtacgttgtt atttgctggg ttggtttgca gataacgttt caccggaagt
    10261 aaaaagcaag ctgccaggta ctgtatccgg gcacggttac attgattttg tcattgatga
    10321 tgtagccgtt gagtttgcag tacgtaaacc aactgcggca cgttccaatg tctccgcaac
    10381 tgtaaatagc actgaagtta aaaagttgat gaagcatgac ggcaaagcct tgcttgtgct
    10441 gtttgacttc tctgacacgc cctattcaga agaacagatt gaatccttcc gcaactggcc
    10501 ctctctcggt cgagggaatc atcgcaagtc cgcattcaac gtcgtttatt tctttgtgga
    10561 aaaacgcaga ccgctgggac tggggaaaat caccaaaaac atccgtatca cttagcaggg
    10621 aattaaataa tgaacagaaa actgttggcc ttattgatgg tagccgtaac gctacctgtc
    10681 gcagcagcac agtacgtaaa acccggagtg gttctaaccc tgaatccggc aaagggcaat
    10741 gctgaattca acattaatgc ctcaatgggg gatggttcgg gcgtatgtaa tatggagggt
    10801 acagcacagc aaatagctgc cggagaaaac cagaagcgtc gctgggtctg gaatgacaat
    10861 accagccagt gtgttgcagt aattagtgaa ctgagaaacg gtaagaccag cattatgact
    10921 cgtggttgcg agggatactg cggggcttct gcggcagccg caatggatgg tctatttaat
    10981 aagaaataaa agtagcttta attaataaag gaactataga tgaaaggaac aatgtttctg
    11041 gtattaatag ctgctggttt gttggctggc tgtggagaga aaacgccaaa atgcagtagt
    11101 gatgatgcaa aaaatctggt cgtggatatt gccagaaaga cgatcgaaaa aggcatgaca
    11161 ctggataagg atgtgcgaat tacagtagaa aatgtcagaa caataagcca tgaatcaggg
    11221 cttgatattt atcagtgcgc tgcagattta acgtttacaa aaccagatct acaaaactct
    11281 ttaccaataa cttaccggat tcagaaaact gatgagggga aaggtcagtt ctatataaac
    11341 gtat
//
LOCUS       DAZFJL010000144        11309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFJL010000144 DAZFJL010000000
VERSION     DAZFJL010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.146"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..1817)
                     /gene="selB"
                     /locus_tag="ACHJ8C_003906"
     CDS             complement(<1..1817)
                     /gene="selB"
                     /locus_tag="ACHJ8C_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO5553689.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVLMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDIHSHHGWLHLPDHKAGFSAEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLR"
     gene            complement(1814..3205)
                     /gene="selA"
                     /locus_tag="ACHJ8C_003907"
     CDS             complement(1814..3205)
                     /gene="selA"
                     /locus_tag="ACHJ8C_003907"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO5553690.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVTKAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3303..3911)
                     /locus_tag="ACHJ8C_003908"
     CDS             complement(3303..3911)
                     /locus_tag="ACHJ8C_003908"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO5553691.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(3982..5118)
                     /locus_tag="ACHJ8C_003909"
     CDS             complement(3982..5118)
                     /locus_tag="ACHJ8C_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO5553692.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNA
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5121..5483)
                     /locus_tag="ACHJ8C_003910"
     CDS             complement(5121..5483)
                     /locus_tag="ACHJ8C_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO5553693.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVAPQGDVPDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6020..7933
                     /gene="mtlA"
                     /locus_tag="ACHJ8C_003911"
     CDS             6020..7933
                     /gene="mtlA"
                     /locus_tag="ACHJ8C_003911"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO5553694.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPVVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAAARRVQEMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            8252..9400
                     /gene="mtlD"
                     /locus_tag="ACHJ8C_003912"
     CDS             8252..9400
                     /gene="mtlD"
                     /locus_tag="ACHJ8C_003912"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO5553695.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNTRH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGTLPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAEISGLDANSEVVSEAVTAYKAMQ"
     gene            9400..9987
                     /gene="mtlR"
                     /locus_tag="ACHJ8C_003913"
     CDS             9400..9987
                     /gene="mtlR"
                     /locus_tag="ACHJ8C_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO5553696.1"
                     /translation="MVDQAQDNLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(9998..10207)
                     /gene="yibT"
                     /locus_tag="ACHJ8C_003914"
     CDS             complement(9998..10207)
                     /gene="yibT"
                     /locus_tag="ACHJ8C_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO5553697.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRQLYRPKQVEEK"
     gene            10491..10853
                     /locus_tag="ACHJ8C_003915"
     CDS             10491..10853
                     /locus_tag="ACHJ8C_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO5553698.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         2758 a         3054 c         2853 g         2644 t
ORIGIN      
        1 cgcaataaat gatcatttcc acgacgacgc gtaaagccaa tacggtcaaa atattccaga
       61 atttgaattg ccagcttgcg gcctacgcct aagcgatcgc ggaaatccgc cgcgcaggtt
      121 gaaccgcact cctgatcgag atcgcggatc atattggcaa attcgacaat ccgatcgtta
      181 cggtaataac gatctttgac gatcgcagta attatccctt gttgcgccgc ctggcgtagc
      241 gtcaggcgca ttgcctgctc gtcggttccc gtctcttttg ccaggtcacg cacccaccat
      301 ggctcgtcac caaacagcgg ctctgctttt tgccagatgg cctgctgctc tgcactgaag
      361 cccgctttat gatccggcag atgcagccag ccgtgatggc tgtggatgtc gccgctttcg
      421 cgcatctttt caatcagcaa cagtaccagc gcttcatctt ccattggcaa cgccatacgt
      481 cgcagacgtt cgcgcccagg accaggttca tcgcgatgtt gctcatgata agtcgctaat
      541 gtgtcgagaa ttttccgctg ccagcgggcg gcaactggcg cattcaacaa gctgtaacca
      601 gcctgaatat agccaggctg ttgcagcagt tcgcgcatcc cttcgccgtt gagctgccgc
      661 gcccaggcga aatccgcaag gttaaccgcg ccgcgttcca gatgaacaga taacgcatcg
      721 gcatcgctct gcgcccgtgc aagagacgcc agccattgca gatattccgg cttacgctta
      781 ccgcggcgcg gcgggttaag catcaacaca cgcgcccccg ccagcgtgtt gcgggccgag
      841 atatcgcgca gcactaagcg atcgttatcc gccagccaca gcggcgtgtc gaacaccagc
      901 tcggcaaggt tatcttccag cagcgaaacg cgtcccgtaa cgtggctggc ggcgtggtga
      961 atatgcagcg gctgccactg ggtcagcggt gtatgggttt gaagctcgac aatcacccgt
     1021 gtgaacggct ccggcggcac atcggcaagc agccagtcgc cacggttaat ctgctctttt
     1081 tccgcatcac ccgcgatgtt aagcgcgata cgctgcccgg catgggcagt ttctgttggc
     1141 tgattttgcg catgcagcgc acgcacgcgc atcggtttat ttacaccagt cagccagagt
     1201 gaatcgccta ctttcacttc cccgcttaac gccgtaccgg tgacgaccag cccggcacct
     1261 tttacggtaa atgcgcggtc gatcgcgagg cggaaactat gttggctggc gtgttcacgt
     1321 tctggtaact gaagcagatg ctcgcgcaga gcatcaattc cccgaccttc ggttgctgcg
     1381 gtgataaaca gttttgcctc agcaaaaccg tattcccgca gcacctcctt tacctggcgt
     1441 tcaacctcat caacacgcgc ttcgtccacg cgatcggctt tggtcagcgc cactgtcagc
     1501 atcgggttac cggtcagctg caaaatcgcc agatgctcac gggtctgtgc catcacaccg
     1561 tcatcgcacg ccaccaccaa cagcgcgtga tcgataccac caacgcccgc cagcatgttg
     1621 gaaagaaact tttcatgacc gggtacgtcg ataaaaccag gcacgcgacc atccggctgc
     1681 ggccagtagg catagccgag atcgatggtc atgccgcgct ttttttcttc cggcagacgg
     1741 tcggcattta cgcccgtaat cgcctgcaac aaggttgttt tgccgtggtc aacgtgtccg
     1801 gcagtcgcaa taatcatttc aacaacatct ccaaaaaccg ttgctcatct tcaaggcagc
     1861 gtaaatccag ccacaatcgt ccgtcataaa tacgaccaat caccggcact ggcaattcac
     1921 gccagcgggc ggctaatgac tcaaggtggc taccgcgtcc atcatgggga gtaaacgtta
     1981 atgccgcgct cggcaggcga tcaaccggca gcgaaccact gccgatctgc gaaagacatg
     2041 gcataacctg taccgcaaac tccgcgccgt aatgtgcggc aaggggagcc tgtaaacgtt
     2101 gtgcctggat ttgaatgacc tctgcgctgc gggtaagcag gcgcagggtc ggtaattttt
     2161 cactcagagc ttcagggtgt aaataaagac gcaacgtggc ttccagcgcc gcgagggtca
     2221 ttttatccgc gcgtaatgca cgcttcagcg ggtggctttg caggcgggcg atcatttctt
     2281 ttttaccaac aataattcct gcctgcggcc cgcctaacag cttgtcgccg gagaagctca
     2341 ccagactgac gcccgccgca atcaactcct gcggcattgg ctctttcggc aaaccgtact
     2401 ggctaagatc gaccaacgag ccactgccta aatcagtcac cactggaaca tccagttctt
     2461 tgccgagcgc caccagttcc gcttcatcta tcgctttggt gaacccctga atgctgtagt
     2521 tactggtatg tactttcatc aacagtgcgg tattttcatt caccgcctga cgataatcat
     2581 tcgcgtgcgt gcggttggtg gtccctactt cgtgtagggt gcatcctgcc tgacgcataa
     2641 catcgggaat acgaaacgcg ccgccaatct ccaccagttc gccgcgagaa accaccacct
     2701 ctttcccgct ggcagtggcc gccaacatca ataacaccgc cgccgcattg ttattaacga
     2761 tacaggcatc ttccgccccc gtaatacggc acagcagctg cgccagcgcc cgatcgcgat
     2821 gtccgcgtcc ggcgtcgtcc agatcgtact cgagggtcac tggcgaacgc atagccttcg
     2881 taacggcttc caccgcggct tccgcctgta aagctcgccc aaggttggta tgcagcacgg
     2941 ttcccgtcag gttgattacc ggacgcagcg cgctttgcgc ctctttcgtc aaccgggcat
     3001 cgacttcttg cgcccagttt tcacaccacg cgggcagcgt ctggctgtct cgaatcactt
     3061 ctcgcgcttc gtcaagcatc tgacgcagca attccaccac gcgggtgtga ccataagtat
     3121 cacgcaaaga aaggaaggag ctatcgcgca ataagcgatc aatagccgga agttgactat
     3181 agagggaacg cgtttcggtt gtcataggaa acctggctga tcaaggccct ctcacacgga
     3241 gaagggcgtt taacataacc acggattgta acgtgagatg ggtcaggagg acatatcgcg
     3301 catcaagcct ttggcggttc ggtgcgggcg aaactttcgc ggctaaacag gttttcgacc
     3361 agtttgacta agtgtgggcg gtcgacacac cagcctggcg caacgcggcg gaaattgaga
     3421 tatccgacgg cacaggcaat ggcaatagtc gccagattga ccgtatcggt tttgagcgtg
     3481 ccatcgacca gatatccttc cagcacatcc agactgcggt tgattttctc acgctgacgt
     3541 aacaattcat cttcagactg ctgcgccgcc ggacgcgcct gttcacgcac cgataccagc
     3601 ccggcatcca taatgccatc cgccagtgcc tcaattttgc gcacccgcaa cgactccagc
     3661 ggatcgcgcg gcaacatcgc cggagcaaca ttcattaatt caatatattc agcgatgatc
     3721 ggtgaatcaa accagcattc gccctcttcg gtcaccagca ccggcacttt tcctaacggg
     3781 ttaaattgcg ccacgccgtt atccgcgtta tagggcagtt cattaataaa ttcgaacgtt
     3841 atgccctttt ctaacaacag aatagaaagt ttgcgtacaa acgggctggt gtagctaccg
     3901 acgagtttca tgccgagtcc tttgtgcgag gaaaaatatc agtatggctc aggctcgcgc
     3961 aaaacacagg tgatacaagt ctcaatgatc caaataaaga taatgcatcc aactggtcat
     4021 tcttagcagc actttccgca tcaccgaaac atggctgaaa tggtcggagt aaactgccac
     4081 ctgggcgtag atgccgtcgg gtaaggcatc gatatcagcg ttagggtcca gttcaatggt
     4141 tcccagcaca ccgtccgtgc cgggcacgac cgttaatgat tgcaataccc cctgcgcctg
     4201 ataagaaccg cctggcacga caggtaaaat actggtcagt ttgccgtgaa acacctgccc
     4261 aggtagcgcg ttaaacacca cttccgcatc atcgccgggt ttcagacgta acagcgagtt
     4321 ctgccgaaat tgggcgacaa tttgccgttt ttgctcgggg atgaaaacca tcaccggacg
     4381 cagcggcaag gcagcggcgt atgtacctgg gcggatcagt acctgagtga cgtagccatt
     4441 gctcggtgcg cgaatgacgg tctgctcaag gttatatttt gcttcagtaa gttgcgctct
     4501 taagctcaca atctgcgatt gctcgccgtt aaccatactg tcgagctggc tctggatctg
     4561 cgcctgctcc gccaccgagc ctttcaccag cgcatcctgc gcgaggaaat tttgccgcgc
     4621 atcgtcgatg tcacgttccg agaacggatt caccgccgcc tggctgcctt tcaggtaacg
     4681 ttgataattt ttaaacagac ggtcgcgctc cgctgaaacc tgggtggtgt tggcctgcgc
     4741 ttcagtgagc tgcgcgcgca gcgtctttat attatgcgtc gccgtcatca ggtcagcctg
     4801 aagcctgtca actcgcgcct ggtaacgaac cgggtcgagc ttaaaaagca cctcgccctt
     4861 ctgaataagc tgattatttt tgtcggtgac ttcagtaaca attcccgtca cctgtggcgt
     4921 gatggggatc gctatcactg ccttttgcgc ggtaaaagtg taagggtggt tgtagttcat
     4981 caacaaaatc aaaccactca ccagaaacac gcctcccagc gccgccgtcg ccagcgtcca
     5041 ctgattcacc ggaatgcgga agattttaaa gaccgcccac gccagcgcca cgtaagttaa
     5101 aacaatcaat agatccataa ttagatctcc ggaaacgtgg aatagtcagt ctttttctcg
     5161 gcggcgagtt ggtgctccag ccgggaaatc cggtcagaaa gtgcggcgat ttccgggtct
     5221 ggcacgtcgc cttgcggcgc aacatgtgac tgcatgcccc agccgcgctc cggttgatag
     5281 agcgtcgccc agatccacaa aaacggccag ataacatgca gagtaaacag gctcacccag
     5341 cccgccgtat gaatagcgtc ggcatgggga tggttgcgct ttttggcaat cagataaggg
     5401 atgtcatgta tcgcgatgat tccataaaaa atcaccagaa agacgaagat cagcactccc
     5461 aacgcgaaat agtttagaaa catatctgcc tcggattcac gtttatcagt gttgtttttt
     5521 ggctggcagc cagaagggag tcaggctgat attttgacaa taatccgggt tcgcgattct
     5581 cgccataaca ccaaagaata attttaagag gtgatgagtt gcttagttac ataacgattt
     5641 catgacgaag gcataacatg ctgtagatca catcaggtga acgccgtaag aaaatatctt
     5701 gtgattcaga tcacaaagat tcaacaaacc atcaaaataa aaatgtgaca taagtcacat
     5761 ttaaatgcca tttttagcga aaatcgccgc cttgttgctt ttttacacaa gcattttgtg
     5821 atgagcatca cgtcaattac ctctctaccc cctacattta tgtgactgat atcacacaaa
     5881 aggccgccga ctggacagtt aaccgattca gtgccagatt tcgcagtatc tacaaggtcc
     5941 ggctacctct gccgccacat taacaaaaaa cctcgggctt ccagcctgcg cgacagcaaa
     6001 cataagaagg ggtgttttta tgtcatccga tattaagatc aaagtgcaaa gctttggtcg
     6061 tttcctcagc aacatggtga tgccaaatat cggcgcgttt atcgcgtggg gtatcatcac
     6121 cgcgttattt attccaacag ggtggttacc gaacgaaacg ctggcgaagc tggtcgggcc
     6181 gatgatcact tatctcctgc cgctgctgat cggttatacc ggtggtaagc tggtaggcgg
     6241 cgaacgtggc ggcgtagtcg gtgccatcac caccatgggc gttatcgtcg gcgcagatat
     6301 gccgatgttc ctcggctcta tgattgcagg tccgctgggc ggctggtgca ttaagcactt
     6361 cgaccgctgg gtagacggta agatcaaatc cggttttgaa atgctggtga ataacttctc
     6421 cgcaggcatc atcgggatga tcctcgctat tctggcattt ctcggcattg gcccggttgt
     6481 tgaagccctg tctaaaatgc tggctgcggg cgttaacttc atggttgtcc atgacatgct
     6541 gccgctggcg tctatctttg ttgaaccggc gaaaatcctg ttcctcaaca acgccattaa
     6601 ccacggtatc ttctcgccgc tgggcattca gcagtcccat gaactgggta aatccatctt
     6661 cttcctgatt gaagctaacc caggtccagg tatgggcgtg ctgctggcgt acatgttctt
     6721 tggtcgtggt agcgctaaac agtctgcggg cggtgcggca atcatccact tcctgggcgg
     6781 tatccacgaa atctacttcc cgtatgtgct gatgaatccg cgtctgattc tggccgttat
     6841 cctcggtggt atgactggcg tgttcacgct gactatcctg ggcggtgggc tggtttctcc
     6901 ggcatctccg ggttctatcc ttgctgtact ggcgatgaca ccaaaaggtg catacttcgc
     6961 taacatcgcg ggtgtgtgtg cggcgatggc agtctccttc gttgtctctg ccattctgct
     7021 gaaaaccagc aaagtgaaag aagaagatga tatcgaagct gctgcacgtc gcgttcagga
     7081 aatgaaagct gagtctaaag gcgcatctcc gctgtctgcg ggcgatgtga ctaacgacct
     7141 gagccacgta cgtaaaatca tcgttgcctg tgatgccggt atgggttcca gtgcgatggg
     7201 tgcaggcgtg ctgcgtaaga aaattcagga tgcaggtctg tcgcagattt cggtcactaa
     7261 cagcgcgatc aacaacctgc cgccagatgt ggacctggtc atcactcacc gtgacctgac
     7321 cgaacgcgct atgcgccagg ttccgcaggc acagcatatt tcgctgacca acttcctcga
     7381 cagcggcctg tacaccagcc tgaccgaacg tctggttgct gcccagcgcc atactgaaaa
     7441 cgaagtgaaa gtgaaagaca gtctgaaaga cagctttgac gattccagtg ctaatctgtt
     7501 caaactgggc gcggagaaca tcttcctcgg tcgcaaagcg gccaccaaag aagaagcgat
     7561 tcgttttgct ggcgagcagc tggtgaaagg cggttacgtt gagccggaat acgttcaggc
     7621 gatgttggat cgtgaaaaac tgaccccgac ttatctgggt gagtctatcg cggtgccaca
     7681 cggtacggtt gaagcgaaag atcgcgtgct gaaaacgggc gtcgtgttct gccagtaccc
     7741 ggaaggcgtg cgcttcggtg aagaagaaga tgacattgcc cgtctggtga ttggtattgc
     7801 tgcccgtaac aacgagcaca ttcaggttat caccagcctg accaatgctc tggatgatga
     7861 gtccgtcatc gagcgtctgg cacacaccac cagcgtggat gaagtgctgg aattgctggc
     7921 aggtcgtaag taatccattc ccaccctctc cgcatgaaga aggtggggct aattgcctga
     7981 tgcgcttcgc ttatcaggcc tacaacataa agcacaattt gttttgtcgg tcggataagg
     8041 tatttacgcc gcatccggca gtcggtgcac aatgcctgat gcgacgctga cgcgtcttat
     8101 caggcctaca ggtcagtgcc aaacacgtag gccggataag gcgtttacgc cgcatccggc
     8161 aaaaagcccc tctcctcacg gagagggttt gggtgaggga aaagcctcac cccagccctc
     8221 tcgggtaaaa acattgatga aggttaatac tatgaaagca ttacattttg gcgcaggtaa
     8281 tatcggtcgt ggctttatcg gtaaactgct ggcagacgcg ggtatccaac tgacgtttgc
     8341 cgatgtaaat caggtggtac ttgatgccct gaatacccgt catagctatc aggttcatgt
     8401 ggtcggcgaa accgagcagg tggataccgt ttctggcgtc aatgccgtca gcagcattgg
     8461 cgatgatgtc gttgatctga ttgctcaggt cgatttagtc actaccgccg ttggccctgt
     8521 cgtgctggaa cgtattgctc cggccatcgc caaagggctg gtgaaacgta aagaacaagg
     8581 taatgaatcc ccactgaaca tcatcgcctg tgaaaacatg gtccgcggca ccacgcagct
     8641 gaaaggccat gtgatgaacg ccctgccgga agatgccaaa gcgtgggtag aagaacacgt
     8701 tggctttgtt gattccgccg ttgaccgcat cgtaccgcct tcggcttcgg caactaacga
     8761 tccgctggaa gtgacggtag aaaccttcag cgaatggatt gtcgataaaa cgcagttcaa
     8821 aggcacgctg ccaaacatcc ctggcatgga attaaccgac aatctgatgg catttgtcga
     8881 acgtaaactc ttcaccctga atacaggtca tgctataacc gcgtacctcg gaaaactggc
     8941 cggtcatcag accattcgtg acgcaattct cgacgagaaa atccgcgcgg tggtaaaagg
     9001 ggcaatggaa gaaagcggcg cggtactgat caagcgctac ggctttgatg ctgacaaaca
     9061 tgcggcatac atccagaaaa tcctcggtcg tttcgagaac ccgtacctga aagatgatgt
     9121 agaacgcgta ggccgtcagc cgctgcgtaa actgagtgct ggcgaccgtc tgatcaagcc
     9181 actgctcgga accctggaat atggtctgcc acataaaaac ctgattgaag gtattgccgc
     9241 tgcaatgcac ttccgcagtg aagatgaccc gcaggctcag gaactggcag cgctgattgc
     9301 cgacaaaggc ccacaagccg cgctggcaga gatttccggt cttgatgcca acagcgaggt
     9361 tgtatccgag gcggtaaccg cttataaagc aatgcaataa tggtggacca ggcgcaggat
     9421 aacctgcgcc cgaataacag attgtcagat atgcaggcaa caatggaaca aacccaggcc
     9481 tttgaaaacc gtgtgcttga gcgtctgaat gctggcaaaa ccgtgcgaag ctttctgatc
     9541 accgccgtcg agcttttgac cgaggcggta aatcttctgg tgcttcaggt attccgcaaa
     9601 gacgattacg cggtgaagta tgctgtagaa ccgttactcg acggcgatgg tccgctgggc
     9661 gatctttctg tgcgtttaaa actcatttac gggttgggcg tcattaaccg ccaggaatac
     9721 gaagatgcgg aactgctgat ggcattgcgt gaagagctaa atcacgacgg caacgagtac
     9781 gcttttaccg acgacgaaat acttggaccc tttggtgaac tgcattgcgt ggcggcgtta
     9841 ccaccgccgc cacagtttga acctgcagac tccagtttgt atgcaatgca aattcagcgt
     9901 taccaacagg ctgtgcgatc aacaatggtc ctttcactga cagagctgat ttccaaaatc
     9961 agcttaaaaa aagcctttca aaagtaagta ctttcgctta cttctcctct acctgtttcg
    10021 gccgataaag ctgacgataa tactccagac gttgtagata taacggcacg ctttcatcag
    10081 gtattccgga cggaatcgcg ttacggggag gtagtttttt cagatactcc cggaacgcct
    10141 ggctggatgc catgaaatct acggctttat cgataagaag cggaacgttt tcacctaatt
    10201 tgcccatgat gatatctccg tattaccccg cgccgggaat gcgcggccgc caattttagt
    10261 ttagatcccg gtataaatga cattaaggaa aacgtgctca atcctcaata atcacctgct
    10321 tctgttgact atcttttttc tttaaatttc ttaactaatc aatgtattgg gtgatttttt
    10381 aacgcataaa tggttacaat ctggaactat tacaatgcca tcagttgtga acaaagcacc
    10441 tggtcgcgca tactagaggc tataaattta tctctatcag aagtcatcac atgaaagaag
    10501 tcgaaaaaaa cgaaatcaaa cgtctcagcg atcgtctgga cgccatccgt caccagcagg
    10561 ccgatctgtc gctggttgaa gccgcagaca aatatgccga actggaaaaa gagaaagcca
    10621 cgctggaagc agaaattgct cgcctgcgcg aagttcacag ccagaaactc agcaaagaag
    10681 cgcagaaact gatgaagatg ccgttccagc gtgcaatcac caaaaaagag caagctgata
    10741 tgggcaaact gaaaaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg
    10801 gccgtgaaat gggcctgcaa gagatgacag ggttctcaaa gactgcgttt taagttcctt
    10861 tctcgtcgct gtaatcacag cctctcacca tttactcgct cctgctggca catgcaggag
    10921 cctgtttcag agatcgaatc tttataacgt tcttaacatt atatattcaa ctccacctac
    10981 acttctgagc gatcgtagca ggctaaattc tgaaaatcag gtacacatta aagaatgatg
    11041 tatcaatatc aacaatgaat ttccgcatat caatgctcga aacataatta attatttaaa
    11101 tttttcaatc agcttcgcaa attccacaat aaaacatcac tcaactgaat atttaccttt
    11161 ccctaccctg tgcctttatc cggtgaaaca tttgacatcg attgacaccg atcattaaaa
    11221 tccgcgcgat taatattatt ggctacaaac tgaaatcgtt tgacatttaa taacatattc
    11281 gcacaaccaa ttaatacaaa aactctcta
//
LOCUS       DAZFJL010000145        11287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZFJL010000145 DAZFJL010000000
VERSION     DAZFJL010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.147"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            20..925
                     /locus_tag="ACHJ8C_003916"
     CDS             20..925
                     /locus_tag="ACHJ8C_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5553699.1"
                     /translation="MRGKIPKTELLVTFEVVARHESYTRAAEELALTQSAVFRQVSAL
                     EEFLHTPLFSHSKKRIFLNDAGKYYLGIVKETLNKLERDTNTIMTWQPTVQVIELAVN
                     PTFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGDFLNREYDAVIMRENFCAPWAEVEY
                     LFDEEILPVCSGSLLAMSDQKLSVAELLTELPLLHQSTRITGWEEWFALSGVSSPLVN
                     NGPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLDSGDMVIPCDVPIRTGNRFIMTWQE
                     EKSDSPHLQQFREWLLAKSVVPQEM"
     gene            complement(959..1458)
                     /gene="cobO"
                     /locus_tag="ACHJ8C_003917"
                     /pseudo
     CDS             complement(959..1458)
                     /gene="cobO"
                     /locus_tag="ACHJ8C_003917"
                     /inference="COORDINATES: protein motif:HMM:NF014614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
     gene            1384..1545
                     /locus_tag="ACHJ8C_003918"
     CDS             1384..1545
                     /locus_tag="ACHJ8C_003918"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553700.1"
                     /translation="MTHRARHSTKRGSGFPFAIAGENNQNPFFLYRRRDARIDLFLQF
                     LLALHMPFS"
     gene            complement(1570..3222)
                     /locus_tag="ACHJ8C_003919"
     CDS             complement(1570..3222)
                     /locus_tag="ACHJ8C_003919"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO5553701.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDPQASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTGTATIVASKGQQIWTGGNDAEALSKGIYSTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDISAVAGDEYHFLCINKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKVAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPAPLREENHAQHVQLDLNRPLRDVMQDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGEPMPDYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDSYVDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCHAGH"
     gene            complement(3330..4609)
                     /locus_tag="ACHJ8C_003920"
                     /pseudo
     CDS             complement(3330..4609)
                     /locus_tag="ACHJ8C_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
     gene            complement(4770..5087)
                     /locus_tag="ACHJ8C_003921"
     CDS             complement(4770..5087)
                     /locus_tag="ACHJ8C_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HFO5553702.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            complement(5084..6454)
                     /locus_tag="ACHJ8C_003922"
     CDS             complement(5084..6454)
                     /locus_tag="ACHJ8C_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HFO5553703.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AREEHLAFSMALIPADVDKAIVHQALDNGKITALDFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKEGAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPQRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCAFKA
                     VNDGEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAGIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            complement(6458..7699)
                     /locus_tag="ACHJ8C_003923"
     CDS             complement(6458..7699)
                     /locus_tag="ACHJ8C_003923"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HFO5553704.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     AVRQAGECVSVQLILENGAVAVGDCAAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATARLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            complement(7699..9144)
                     /locus_tag="ACHJ8C_003924"
     CDS             complement(7699..9144)
                     /locus_tag="ACHJ8C_003924"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HFO5553705.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEI
                     SMASGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNTGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARFEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            complement(9163..10551)
                     /gene="glmL"
                     /locus_tag="ACHJ8C_003925"
     CDS             complement(9163..10551)
                     /gene="glmL"
                     /locus_tag="ACHJ8C_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HFO5553706.1"
                     /translation="MQIVSVDIGSTWTKAALFAREGDALTLVNHVLTPTTTHDLAKGF
                     FSSLNLVLNVDNALPLFNNGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYSYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPLAARQAICDIFLKRIVKGKGLDV
                     VVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAMVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            complement(10551..11000)
                     /gene="glmS"
                     /locus_tag="ACHJ8C_003926"
     CDS             complement(10551..11000)
                     /gene="glmS"
                     /locus_tag="ACHJ8C_003926"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HFO5553707.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHGFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
BASE COUNT         2682 a         2831 c         2830 g         2944 t
ORIGIN      
        1 atttttaagg gaaggcatta tgcgaggaaa aatacctaaa acggaactgt tggtgacttt
       61 tgaggtcgtc gcacgtcatg aaagttatac cagagcggca gaggagttag cgttaaccca
      121 aagtgcagta ttcagacaag taagtgcgct ggaggaattt ttacatactc cgttattttc
      181 acactctaaa aaacgcattt ttcttaatga tgcagggaag tattacctgg ggatagtaaa
      241 agaaacatta aataagctgg aacgcgatac caataccatt atgacctggc agcctacggt
      301 tcaggttatt gaacttgccg ttaatcctac ttttagtacc cactggttaa tcccaaattt
      361 acatgaattc acaaagttac atcccgatat tattgttaat attcattctt tagctaataa
      421 cggcgatttt cttaatcggg agtacgatgc tgtcattatg cgcgaaaatt tttgcgcgcc
      481 ctgggcggaa gtagaatatc tctttgatga ggagattttg ccggtatgca gcggcagttt
      541 gcttgcaatg tccgatcaaa aactgtcagt ggcagaactg cttactgagt taccacttct
      601 gcatcaaagc acccgtatta ctggctggga agagtggttt gctttatctg gcgttagcag
      661 tccgctggtc aataacggac cacgtttcga tttgctttca atgctgattg ccgccgtgcg
      721 ctctaatctc ggcgtggcgc tgttaccacg gttcgctatt caacatgatt tggatagtgg
      781 cgatatggtg attccctgtg atgtgcctat tcgtaccggt aatcgtttta ttatgacgtg
      841 gcaggaagag aagtctgact cgccgcactt gcagcaattt cgtgaatggt tgctggcgaa
      901 atcggtcgta ccgcaggaga tgtaaaacct gcgcctggcg ggatgccagg cgcagttatt
      961 accagtcgat acccggctgc gcctgaatgc cgttatcaaa agcatgtttg acggggcgga
     1021 tttcgctgac cgtatccgcc atttttaata tctgactgtg gcaaccgcgt ccggtgacaa
     1081 tgacactttg ttgtgccggg cggttttgca gagaagcgat cacttcttcg gtatccagat
     1141 aatggtaagc cagcatatag gtcagctcat caagcaccac taagtcgtaa cgcttgtcag
     1201 ccagcatccg tttgctttcc gaccagacct ctttcgccgc gtcgatatcc gcttgccggt
     1261 tctgtgtctc ccaggtaaaa ccggttccca taatatgaaa ctcaaccccc agcggctgga
     1321 gaaggttgta ttcgccgttg tcccattgcc ccttgatgta ctgcgcaacc ccaccgtttt
     1381 gccatgaccc accgcgcgcg tcacagtacc aaacgcggca gtggatttcc ctttgccatt
     1441 gccggtgaaa acaatcaaaa tccctttttt ctctatcgcc gccgcgacgc gcgtatcgac
     1501 ctgttccttc aatttctgct ggcgctgcac atgccgttca gttgaaattc gcgcttccat
     1561 tcattttcct taatgtcccg catggcaact ggcgcagcgg tgttgctgct caaactgact
     1621 aaagaaatta ttgcctttat catctaccag gatgaatgcg ggcagatttt ccacttccat
     1681 catccacacc gcttccatac ccagctccgg gtattccagg caacgcagac ttttgacata
     1741 ttcttgcgcc agtaacgcag ctgcaccgcc aatacttccc agattgaagc cgccatgttt
     1801 gtgacaggca tcggtgacct gctggctgcg gttacctttc gataacatca ccagactgcc
     1861 gccagccgcc tggaaggtat cgacataact gtccattcga ccgcccgtgg ttggacccag
     1921 tgaaccgcag gccatgttct ccggtgtttt cgccgggcca gcgtaataga caatatgatg
     1981 cttcaggtaa tccggcatcg gttcgccgct atccagccgc gcttttattt tggcgtgggc
     2041 gatatcgcgc gccactacga tgggaccact taacgacacg cgggtgccta ccggtaatct
     2101 ggcgagatcc tgcatcacgt cacgcaacgg acgatttaga tccagctgta catgttgcgc
     2161 gtgattttct tcccgcagtg gggctggaat atattgcccc ggattgtgtt cgagtttttc
     2221 cagccagata ccgtgcttgt taatttttgc tttgatattg cgatcggcag aacaggagag
     2281 cgccatagct atcgggcagg aaccaccgtg acgcggcaga cgaatgacgc gaatatcatg
     2341 agcgaaatat ttacctccaa attgcgcacc aataccaaat tgctgactgg cctcgagcaa
     2401 cactttttcc agctcaatat cacggaatgc ttgtccctgc tcgtttccgc tggtgggcaa
     2461 attgtcgtaa tatttcgtag aggccagctt ggcaactttt aaggtttgat cggctgaaag
     2521 ccctccgacg acaaaggcga tgtggtaagg tgggcaggca gcggtgccca gcgatttcat
     2581 tttttcgatt aaaaatgcgg tcagtttttc tggttgcaat agggattttg tttcctgata
     2641 aagcgcggct ttattggcag agccgccccc tttattaata cagagaaaat ggtactcatc
     2701 accagcgacg gcgctgatat caatctgcgc cgggagattg gtttgcgtat tgacttcggt
     2761 atacatatcc agcggcgcat tttgtgaaaa gcgcagatta ttttcctgga aagtactgta
     2821 aatgcctttg cttaaggctt cagcgtcgtt acctcccgtc catatctgtt gccctttact
     2881 ggcaacaata gttgcggtgc cggtatcctg acagttgggt aatacccctt ttgctgagac
     2941 ttcagcatta cgtaataact gcaacgcaac gtatttatca ttactactgg cctgcggatc
     3001 gttaagaatg ctggcgacct gttgcaaatg agcggaacgt aagaaaaaag aggcttcata
     3061 aaatgcctgc cgggcaagca gcgttaatgc ttcaggagcg atttttatca cttcttcgcc
     3121 gtctaattcc gttactgtaa tatgctgatc gctaattaat gtatattctg taccgtcttt
     3181 attttgtaga aacggttctt gccagataaa tggtttggac atatttcact tttcacctta
     3241 aacgttagtg ttacttaatt gccgtctaac ttcatgatcc ggcttataaa aatgcgtagt
     3301 cattggcttc tgattgctta agatccccct cagggcagag caacgttcga ttcattgggt
     3361 tttaatgctt taccccgaca accctcgccg aaatgttcgc cttcccaccg actcaccatc
     3421 gcagcgccca ttgcgttgct catgacgttt gtcgcggaac gccccatatc aaggaacgga
     3481 tcaacgccca tcagcaagat cagccccgct tccggaatat tgaactggtt gagcgtggcg
     3541 gcgataacca ccattgaggc gcgtggtacg ccagccattc ctttcgaggt caacatcagg
     3601 atcaacagca tggtgatttg ctcaccgatg gataaatgga tattgcaggc ctgggcgatg
     3661 aaaactgtgg cgaaggagca gtaggccatt gatccaacga gattaaatga gtagccaatg
     3721 ggtaagacaa agctggcaat tttgggggaa acgccaaatt gctccagttt ttcaagcgtt
     3781 cccggaaaag ccgcttcaga actggatgtg gtaaatgcca gcagggcggg ttccgaaagg
     3841 gcgcgggtca ggcgtctgat gcaggggccg acataaacga tggccagacc gataagcagc
     3901 acccaaagta ataacatggt gaaataaaat tcacccataa agatcccggc gctcaccata
     3961 actgccagtc ctcgttcagc aatcaatgct gaaatagcag cgatacggtc aggggagcga
     4021 agagcatgac gtagccagtg agctttaaca tggcatgtgc cagcgaatct aaggcgtgaa
     4081 cgatggcgct gcctttctcg ccaatcgccg tcaggctaca gccgaggaaa attgagaaca
     4141 ccacgatttg caaaatttca ttgtgcgcca tggcatcgac aatgctggtg gggaaagcat
     4201 gcgaaataaa tacttttagc gtaaagggtt ctgacgcgac cactccggtg gtttcggctc
     4261 cgtgtgcaac aaaattaatt cctgtgcctg gcatgaagaa atttactgtt atcaagccta
     4321 aggcaattga cagcaatgag gcgcaaataa ataaaaagag tgttttagaa aaaatacgac
     4381 caagggcttt ggcatctccc attttagcta tacctaccac cagggtagag accactaacg
     4441 gagcgataat catttttatc agtcgtaaga aaatcgtcgt gaatatcgat atttcttgcg
     4501 catagagttt tgcggtttcc ggtgaagcag tgttattgag cactacgccg ataatcattc
     4561 caagtaccag cgccaaaaga atcatcgtgg ttaaacttat tttcttcatt ttttcctcca
     4621 tcaggtgcac ggcgataaat cagaatcagg agaagtagat gcctgaatag agattcaggc
     4681 atctacctgc tattacaacc ttacaaatag ctgaagtgtt gaactgcaat aagtgacggg
     4741 atgtcagttg gctaaaagta ttcggatttt tacaaatcga aactcatatt taataaaggt
     4801 gcatgttgct gtgcaccata gacatcattg tcacctacat tcccggagat aatatctcgt
     4861 ggcatgacga tttttactgc gttagaagga tcaaaccaga caatacggtg aataaagtca
     4921 ggttcaacat gatataagcg cgcaatcagt tctggggtta attgccctga tgatttaact
     4981 cgctcatagt tttctttcgt tttaaataaa atatctaaaa ccagttcgta aggtccggca
     5041 tttttcgaac gaatgacttg cgccagtgag acaattgact gtttcatgcc tgaactcctt
     5101 ctggcgtcac ctgttcaata tggaaatcaa aacgcagagc gtcgttggct tcaatcaggt
     5161 gatatatgga aaattcgtat accggcccgc cctggatatc agaaggggag aacgggaacg
     5221 ccagattgcc tgcggtagcg atgcggtttt catagccgta gtgcagcatg gtagagcgca
     5281 ccagcgagca aacgctattg gcaatttcct gggtcggtgc aactacatct aggacaattc
     5341 ccagctcatg cccggcagtt tgcatcggtt catggtcgcc catcaccccg tttttgccgt
     5401 acaggtggaa attgatgcga atgctgtcat cgtcgagcga caggttacgt gaaacgctgg
     5461 ttttgacttc atcgatgatt ttatcgatcc cggcgatcat gatcgggtcg cgtgttccgg
     5521 cgatagtcag acagcggaag cccacccgtc gcgcaccttc cagtttcagg gcatacggcg
     5581 tttcttcatg cttagaaccg ctgacgtaca cttcgccgtc attgaccgct ttaaatgcgc
     5641 agcctttcag attcagaaca ccacctggtc ccggcaggaa gtacgggtcc gatttctcat
     5701 acagcgtatg cgcggctgcg gatgtttcgg tgaatttacg ctgcggattg aacgtcttca
     5761 gcgtgaagcc attatcgtcg atgatgccca ttgcacagtc agagccagat cctggtgtcg
     5821 cggcaattgc tgcgcattcg aggattttcc cacaatgcag cgccagccct tcgtcaaagc
     5881 cttgcatgat gggtagcgca gcaaagcagg ccgggtcgta agcgcgcccg cccagcacca
     5941 cttgcgcgcc ttctttcagc gcccgctgga agggttcgat gcccatttgc gcgacgatat
     6001 acgtactttc gtcaatcgcg tcgtgggtta acggcgggac aaaatccagc gccgtgattt
     6061 tgccgttatc cagcgcctgg tggacgatgg ctttgtcaac gtctgccggg atcagcgcca
     6121 tcgagaatgc gaggtgctct tcccgggcaa tctcatgaat tatttctcgg caccactcca
     6181 ggtgtggtgc ggcaccagaa ccgccggccg ttccgatcac caccggtata ttattttgca
     6241 cgcctgcggt gatcatatag cgcagatcgc gcttgacccc tgcgcgatcg gtaaacggtt
     6301 tgcctgcccc gaggtagtgg gggcctggat cggaagagcc tgcatcaaca gcgatcagat
     6361 cgggtgattc tgccatcgct ttgcggaagc tctcttccgg gaatccatac ccgaggattg
     6421 ctgttggcga taagacttta aaagaacgag ccatttgtta atccttagcc tgcaacagcg
     6481 cgatagtacg attcatttcg ttgaagacaa tatcgaggcc ttcgtcaaag cccatcccag
     6541 gttttaccag catacgcata gggcgagcgg caagggcgac gtggacacag gtgcgggcac
     6601 tgacatcagt ttcattgcag gtgccgccct ggtacgcttc catgctgtgg ctgttgcagt
     6661 aaagaaccgc atcgacgatg ttgtgaatac tgcccagatc cggggttttg atttgcacca
     6721 tgtggcaact ggcggcatcg gtgaaatcaa caatatcctg gtaggtgtta caccattcat
     6781 cggccacaat tttcacgccg gaaccgaggc gcgtcagctc tttggtaatc gcggtcagca
     6841 agcgaatttg atcgggcttg ttaccggcat cgaccggccc ttcaatgtag agcggcaggc
     6901 cttgcgcttc tttttccagg ctggcgatgt attgcgcaca gcgaagcgga tccatatcga
     6961 agatcagacc gatagtgccg tatacatcaa tgtgcagggt agggtggtag cgtgcgctgg
     7021 tgcgcttgct cagaatgcga tccgacaacc agcggacata ttcgcgcagt ttttcacctt
     7081 taaagcccag tttctcttcg acgttattaa tcagtgcatg gggcagcacg tcgatgcctt
     7141 taaggatcat cttatcgacg gcgatatatc gatcgtcccc gctctgacca aataatggaa
     7201 tggattccgc cacgcgtggc aactgccatt catcacagac cacttcagtt ttcagacggg
     7261 cggttgccag cgcggtagca tcaagcagcg cctgtgataa tccgtagcgc acggcggtat
     7321 gcagcaagtt gccgtcaata cgcaatttgt cgaagaaacg ggcattcggc aggaaagcat
     7381 ccacatcgcg gcctaccagt aatggcttga tatggtcgtt gaggaacgga ataaagtgct
     7441 ctgcgaggaa cagtggatcg cgaccacctg ccccggaata ctgtacggca gcacagtcac
     7501 ctacggcgac cgcgccgttt tccagaatca actgtaccga tacgcactcc ccggcctgac
     7561 gaacggcgtt aaacccctgt gttaccggct ccccggtata aaaaaagccg tcatgacccg
     7621 ctccgttttt tatcgcctgc tggtcatcga aatagaatga ggagtagcca gcggtgaaca
     7681 gagcctgttt tattttcatt atggtcttcc tattaattta ctgtgggata cagcattgat
     7741 gtcatctaca accatctgga atgtcggttg gcgtccttca aagcgtgcgc gttccgcaac
     7801 gtaatcgtgg tggaggtcga gaatatcttt tggcaatgga acggctccgg cttccagtac
     7861 gcgtatcgcg ccggtgttgt cacggaccgg aagaattttg cccgcgttgc aggcggctgg
     7921 cgcaaacggc acatccagta cgcccgcttc aaatgccagc accgtaccgc gcgcaatatc
     7981 accattaccc agttcgaaca ctttattcag taccgcgcga acctcgctct taatcagttc
     8041 aatttccagt tcaaccgcag ggcacggcgg gaatttctgc tcgttgacca tgttgagcat
     8101 ctggcgggag gctttcagcc cctgaatgtt ggcagcggca gtcgggatgc cccaggcttc
     8161 gtgcggactt ttggtgatga ctttggtggc accagacatc cccgcgacgg ctgcgcccca
     8221 ggagataata gcgaacgctt tcgattcgtc ttccggaaag ccgcccatcc actggtgaaa
     8281 cacggtactt aattcatagt cggtatagcc atagctctga aaatattcgt gtgcaagttc
     8341 acgcagagac tggatcgccg caacatcctg cgtcaggctg ccgacctgtc cataaccaac
     8401 ggtgatagat ttcacaccct gttccagcgc cagtaatcct tcgattatcg cgatagagtg
     8461 cgagataaac ggcgggatca gggtacccgt tagtgggcca aacggttcgc ggttgatgcg
     8521 gatcccgtgt tcttcataca tccccattaa acggtcgcag tactgccagt cgcggatcga
     8581 tttttccagc gttacgcgct tggcatacgg aatattgtag gaaatgccgc cgccttcata
     8641 actggtaaag ccgctggcca tcgagatttc agccagcaga cgcgcatctg gcgtgccgtg
     8701 gcggatctgt agcggttttt gcagggtttc ggtgaggcga cggcaagctg cgacgccgtg
     8761 gttaacgacc ggtaaaccgt tcagtttaga ggttccggct tcgatggatt ttttaatccc
     8821 tacagccgct tcttcgtacc gattcagacg ggtatatgca tcgatggtgc tcggcagcag
     8881 atcgcactct tcttgtagtg ttttcagtag ttcgatgtgt tcgtccatca gcgccacgcc
     8941 tgcgcgcggt tggctaagag ttttaccttc cttgtctgct ttcagcaggg caagagagaa
     9001 gcgtttatgc tcaggaatgg tttgttggta tttaacgcca tcttcaaagt tttcaacatc
     9061 cttgccggtt tcccaggttt tcagtacctg ttgccgctcg gtcatgaatt cgtcatgggt
     9121 taatttctta tttcgaagtt ccattgattg cagccttaat tgttataggg ttaagcattg
     9181 aatactggtg cgcgcggcgg ccagcggatc gactctggcg acgtttgcca gcagtggtaa
     9241 tagcccccgg gaatcgcggt aatattcaaa ttcggtcggt aagagaatgc gtttaccgtc
     9301 gtcgtttaac tcgcggtatt tcaaccagtg atgcatatcg aactgactgg cacgcgagag
     9361 ccatccgccg gatccgacga ctttacgcac ggttgtcagg tcacgtccca tctgtaaatc
     9421 gacattgccg acgcaggtgc agacctcttt tttggtgccc gcatgtcttt cggtggcata
     9481 acccacacac agtccggcca ggactgtatc gaaatatttt tcttctgcat tcgcgggtaa
     9541 gtagtcggga tgagcgacta gatgacggag gtaatgataa aaagcgtcca gctgtgcggg
     9601 ttgctgagca aaattcacat tgactaactc tttcgcactt tcaccaacca ccattgccga
     9661 gacgcgcatt cccagatcgc cttccaccgt ccgtttcacg aagggttccg gtacgccgtg
     9721 taaaacggtg tcgggcgaga gcgtgttggc acaggcggaa tagacatcgg tggttgcgcc
     9781 acccatgtcg atcagcatga actctttcca gctgtgatcg acattactga ttgccttaac
     9841 cagttcaaag actgtccacg gtgtcggcat tggttcttcg cctgttttat caacaacgac
     9901 atccagccct ttccctttaa cgatgcgttt aaggaatatg tcgcagatag cctggcgtgc
     9961 agctaaaggg ttcggatggt caagatcggg cagaacgttg tcgacgatgg tcaggtcttt
    10021 atgccctagg atctcctgca cctcgtcctg aatatcccgg tttccggcat aaataatggc
    10081 gcagtcgagt ttggattctg ccagcacacg ggcgttattc aggccgtagc tttcttctcc
    10141 gccgtcggtg ccgccggtga acaagagaat atcgggctgc gtttcttcca gtgcctggat
    10201 atcacggcgg ttcagcttgt aggagtaata ttgcgcaatt ttagcccccg cagagtgagc
    10261 ggttactttc gcggtttcca gggtgataga aggcaccaat cccattgcgg caaccgccag
    10321 cccacctttg gcagaggagg aatacttcaa tgcaacctca ccgttgttaa acagggggag
    10381 ggcattatct acgtttaaca ccaggttcag gctggagaaa aaccccttcg ccagatcatg
    10441 ggtggtggtt ggggtcagta cgtggttaac caacgttaac gcgtccccct cccgggcgaa
    10501 gagggctgct ttggtccacg tcgatccaat atcgacagag acgatttgca tcagattgcc
    10561 tcttcaagaa tacgagtatc aacgtcatga cgttggttga tatcgtgtgc catcagctga
    10621 caaacatctt ctaagtcatg acttggggca aagacgcgat caaatcccat ctctttgaat
    10681 ttggtttcca cgtcagcgaa gtcatgtttt ccgaccacca ggttaccgcc cacatagagc
    10741 aggatgtccc ccagtccacg ctcgatgcag cgttcgcgca tgcccagaca gtcgatatcg
    10801 ccatgcccat agatagagga gacgacaatc gcgtcagcgc cagtttcaat ggccgcatca
    10861 atatattcgt cctggctgac cattacgccc agattaatga ctctgaagcc atggttgcta
    10921 aaaacgcggt ccagaacttt attgcctact gcatggcagt ccgcgccaat aacgccaatc
    10981 acaagtgttg ctttcttcat gggtaatcct ttgtaagaca gagagtaaaa taagttacac
    11041 acgtcgtatt tatggccgtt cataacggca caggtttaat atcacaggag tgtctttatc
    11101 tgaccgtaat gttgcaattg atctattttc aaaatgtctt tcgggaaatg attgtttaaa
    11161 gtatgttgtt aaattctcat gttaattcag tgtgttatgt caactaatgt catgaatgtt
    11221 aaatttttaa aattaacttg atttggtgta ttgaggtatt tgtgatctac ttctaatttg
    11281 catttta
//
LOCUS       DAZFJL010000146        11233 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFJL010000146 DAZFJL010000000
VERSION     DAZFJL010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.148"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..466
                     /locus_tag="ACHJ8C_003927"
     CDS             <1..466
                     /locus_tag="ACHJ8C_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO5553708.1"
                     /translation="QIARAFGKRHQHVIRAIKCLRCSEEFSTTHFWAVEKINDLGIFD
                     KKQIYYRMDFSGFVMLVMGFNGAKADAVKEAYINAFNWMSAELRKYSESYEAERNAVM
                     LEYMKEKDVASMSGRLLNRWGRTKKPQLLAKLERLERQGQFLLPGFDKGIQA"
     gene            666..815
                     /locus_tag="ACHJ8C_003928"
     CDS             666..815
                     /locus_tag="ACHJ8C_003928"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553709.1"
                     /translation="MRPWSHDAGLSDIEFIANKPERLRVLSGDPAGYGAATSRVFAIY
                     ENFPV"
     gene            955..1500
                     /gene="nohD"
                     /locus_tag="ACHJ8C_003929"
     CDS             955..1500
                     /gene="nohD"
                     /locus_tag="ACHJ8C_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO5553710.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1475..3400
                     /locus_tag="ACHJ8C_003930"
     CDS             1475..3400
                     /locus_tag="ACHJ8C_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO5553711.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGKEQYLKFGDKETPFGLKWMPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3397..3603
                     /locus_tag="ACHJ8C_003931"
     CDS             3397..3603
                     /locus_tag="ACHJ8C_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO5553712.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3600..5201
                     /locus_tag="ACHJ8C_003932"
     CDS             3600..5201
                     /locus_tag="ACHJ8C_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5553713.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMISPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRDRLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5182..6501
                     /locus_tag="ACHJ8C_003933"
     CDS             5182..6501
                     /locus_tag="ACHJ8C_003933"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO5553714.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRQMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATALQADVTDVVSAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAGTPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6511..6843
                     /locus_tag="ACHJ8C_003934"
     CDS             6511..6843
                     /locus_tag="ACHJ8C_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO5553715.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMPDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAATDETKKRTAF
                     AGTAISIV"
     gene            6899..7924
                     /locus_tag="ACHJ8C_003935"
     CDS             6899..7924
                     /locus_tag="ACHJ8C_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO5553716.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMILRRLPDEDPQNLA
                     DPAYRRRRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            7966..8361
                     /locus_tag="ACHJ8C_003936"
     CDS             7966..8361
                     /locus_tag="ACHJ8C_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO5553717.1"
                     /translation="MTKDELISRLQVLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPGTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVATEMTERGLARMQ"
     gene            8373..8726
                     /locus_tag="ACHJ8C_003937"
     CDS             8373..8726
                     /locus_tag="ACHJ8C_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO5553718.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            8738..9316
                     /locus_tag="ACHJ8C_003938"
     CDS             8738..9316
                     /locus_tag="ACHJ8C_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5553719.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARIVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9313..9708
                     /locus_tag="ACHJ8C_003939"
     CDS             9313..9708
                     /locus_tag="ACHJ8C_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO5553720.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEIFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            9716..10456
                     /locus_tag="ACHJ8C_003940"
     CDS             9716..10456
                     /locus_tag="ACHJ8C_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5553721.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSGNGELAAVAEITVTDS"
     gene            10472..10894
                     /locus_tag="ACHJ8C_003941"
     CDS             10472..10894
                     /locus_tag="ACHJ8C_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO5553722.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTENAGPAEPVSAGKCSTVS"
     gene            10921..>11233
                     /locus_tag="ACHJ8C_003942"
     CDS             10921..>11233
                     /locus_tag="ACHJ8C_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO5553723.1"
                     /translation="MGRPDWRAMLAGMSSTEYADWHRFYSTHYFHDVLLDMHFSGLTY
                     TVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAGGVRFGPDGNEVIPAS
                     PD"
BASE COUNT         2571 a         2880 c         3419 g         2363 t
ORIGIN      
        1 ccagattgcc agggcatttg gtaaacgcca tcagcatgtg attagggcta ttaaatgttt
       61 gagatgttct gaggaattct cgacaaccca tttttgggcc gtcgagaaaa tcaatgactt
      121 agggattttt gacaagaaac agatttacta ccgcatggac tttagtggct tcgttatgct
      181 ggttatggga tttaacgggg caaaagccga tgctgttaaa gaagcctata tcaatgcgtt
      241 taactggatg tcagcagaac tccgtaagta cagcgaaagt tatgaagcag aacgtaacgc
      301 cgtaatgctg gagtacatga aagagaagga tgtcgccagc atgtcaggcc gtctgctcaa
      361 tcgctggggg agaacgaaaa aacctcaatt gcttgcaaag ctggaacgtc tggagagaca
      421 gggacagttt ttattaccgg gattcgataa aggtattcaa gcctgacaca ttatgcgctg
      481 tatcgtcgcc gtattcccgc attaaccatg accgtagccc gacggggaat tccttctgcg
      541 cgagtgtgcg ggaataatca aaacgatgca caccgggttt ttaccgcgct aatgattcgc
      601 gggtttgccc tcatgctcgc cagtcttgtg cgagggtgga agaaacaggg catgtattca
      661 ggagcgtgcg accgtggtcg cacgacgctg gtctgtcaga cattgaattc attgcgaata
      721 aaccagaacg cttgcgggtc ctttccggcg atccggcagg ctacggggcg gcgacctcgc
      781 gggttttcgc tatttatgaa aattttccgg tttaaggcgt ttccgttctt cttcgtcgta
      841 acttagtgtt tttatttaaa ataccccctg aaaagaaagg aaacgacagg tgctgaaagc
      901 gagctttttg gcctctgtcg tttcctttct ctgtttttgt ccgtggaatg aacaatggaa
      961 gtcaacaaaa agcagctggc tgacattttc ggtgcgagta tccgtaccat tcagaactgg
     1021 caggaacagg gaatgcccgt tctgcgaggc ggtggcaagg gtaatgaggt gctttatgac
     1081 tctgccgccg tcataaaatg gtatgccgaa agggatgctg aaattgagaa cgaaaagctg
     1141 cgccgggagg ttgaagaact gcggcaggcc agcgagacag atctccagcc agggactatt
     1201 gagtacgaac gccatcgact tacgcgtgcg caggccgacg cacaggagct gaaaaatgcc
     1261 agagactccg ctgaagtggt ggaaaccgca ttctgtactt tcgtgctgtc gcggatcgca
     1321 ggtgaaattg ccagtattct cgacgggatc ccactgtcgg tgcagcggcg ttttccggaa
     1381 ctggaaaacc gacatgttga tttcctgaaa cgggatatca tcaaagccat gaacaaagca
     1441 gccgcgctgg atgaactgat accggggttg ctgagtgaat atatcgaaca gtcaggttaa
     1501 caggctgcgg cattttgtcc gcgccgggct tcgctcactg ttcaggccgg agccacagac
     1561 cgccgttgaa tgggcggatg ccaattacta tctcccgaaa gaatccgcat accaggaagg
     1621 gcgctgggaa acactgccct ttcagcgggc catcatgaat gcgatgggca gcgactacat
     1681 ccgcgaggtg aatgtggtga agtctgcccg tgttggttat tccaaaatgc tgttgggtgt
     1741 ttatgcctac ttcatagagc ataagcagcg caacacactt atctggttgc cgacggatgg
     1801 tgatgccgag aactttatga aaactcacgt tgagccgacc atccgtgata ttccttcgct
     1861 gctggcgctg gccccgtggt atggcaaaaa gcaccgggat aacacgctca ccatgaagcg
     1921 tttcaccaat gggcgtggct tctggtgcct gggcggtaaa gcggcaaaaa actaccgtga
     1981 aaagtcagtg gatgtggcgg gttatgatga acttgctgcc tttgatgagg atattgaaca
     2041 ggaaggctct ccgacgttcc tgggcgataa gcgtattgaa ggctcggtct ggccaaagtc
     2101 catccgtggc tccacgccca aagtgagagg cacctgccag attgagcgtg cagccagtga
     2161 atccccgcat tttatgcgtt ttcatgttgc ctgcccgcac tgcgggaagg agcagtacct
     2221 taaatttggc gacaaagaga cgccgtttgg cctcaaatgg atgccggatg atccctccag
     2281 cgtgttttat ctctgtgagc ataatgcctg cgtcatccgt cagcaggagc tggactttac
     2341 tgatgcccgt tatatctgcg aaaagaccgg gatctggacc cgtgatggca ttctctggtt
     2401 ttcgtcatcc ggtgaagaga ttgaaccgcc tgacagtgtg acctttcaca tctggacggc
     2461 gtacagcccg ttcaccacct gggtgcagat tgtcaaagac tggatgaaaa cgaaagggga
     2521 tacgggaaaa cgtaaaacct tcgtgaacac cacgctcggt gagacatggg aagcgaaaat
     2581 cggtgaacgt ccggatgctg aagtgatggc agagcggaaa gagcattatt cagcgcccgt
     2641 tcctgaccgt gtggcttacc tgaccgccgg tatcgactcc cagctggacc gctacgaaat
     2701 gcgcgtatgg ggatgggggc cgggtgagga aagctggctg attgaccggc agattattat
     2761 gggccgccac gacgacgagc agacgctgct gcgtgtggat gaggccatca ataaaaccta
     2821 cacccgccgg aatggtgcag aaatgtcggt atcccgtatc tgctgggata ctggcgggat
     2881 tgacccgacc attgtgtatg aacgctcgaa aaaacatggg ctgttccggg tgatccccat
     2941 taaaggggca tccgtctacg gaaagccggt ggccagcatg ccacgtaagc gaaacaaaaa
     3001 cggggtttac cttaccgaaa tcggtacgga taccgcgaaa gagcagattt ataaccgctt
     3061 cacactgacg ccggaagggg atgaaccgct tcccggtgcc gttcacttcc cgaataaccc
     3121 ggatattttt gatctgaccg aagcgcagca gctgacggct gaagagcagg tcgaaaaatg
     3181 ggtggatggc aggaaaaaaa tactgtggga cagcaaaaag cgacgcaatg aggcgctcga
     3241 ctgcttcgtt tatgcgctgg cggcgctgcg catcagtatt tcccgctggc agctggatct
     3301 cagtgcgctg ctggcgagcc tgcaggaaga ggatggtgca gcaaccaaca agaaaacact
     3361 ggcagattac gcccgtgcct tatccggaga ggatgaatga cgcgacagga agaacttgcc
     3421 gctgcccgtg cggcactgca tgacctgatg acaggaaaac gggtggcaac ggtacagaaa
     3481 gacggacgga gagtggagtt tacggccact tccgtgtctg acctgaaaaa atacattgca
     3541 gagctggaag tgcagaccgg catgacacag cgacgcaggg gacctgcagg attttatgta
     3601 tgaaaacgcc caccattccc acccttctgg ggccggacgg catgacatcg ctgcgcgaat
     3661 atgccggtta tcacggcggt ggcagcggat ttggtgggca gttgcgggcg tggaacccac
     3721 cgggtgaaag tgtggatgca gccctgctgc ccaactttac ccgtggcaat gcccgcgcag
     3781 acgatctggt acgcaataac ggctatgccg ccaacgccat ccagctgcat caggatcata
     3841 tcgtcgggtc ttttttccgg ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg
     3901 aggaagaagc ccgtgccttt tcccgcgagg ttgaagcggc atggaaagag tttgccgagg
     3961 atgactgctg ctgcattgac gttgagcgaa aacgcacgtt taccatgatg attcgggaag
     4021 gtgtggccat gcacgccttt aatggtgaac tgttcgttca ggccacctgg gataccagtt
     4081 cgtcgcggct tttccggaca cagttccgga tgatcagccc gaagcgcatc agcaatccga
     4141 acaataccgg cgacagccgg aactgccgtg ccggtgtgca gattaatgac agcggtgcgg
     4201 cgctgggata ttacgtcagc gaggacggct atcctggctg gatgccgcag aaatggacat
     4261 ggataccccg tgagttaccc ggcgggcgcg cctcgttcat tcacgttttt gaacccgtgg
     4321 aggacgggca gacccgcggt gcaaatgtgt tttacagcgt gatggagcag atgaagatgc
     4381 tcgacacgct gcagaacacg cagctgcaga gcgccattgt gaaggcgatg tatgccgcca
     4441 ccatcgagag tgagctggat acgcagtcag cgatggattt tattctgggc gcgaacagtc
     4501 aggagcagcg ggacaggctg accggctgga ttggtgaaat tgccgcgtat tacgccgcag
     4561 caccggtccg gctgggaggc gcaaaagtgc cgcacctgat gccgggggac tcactgaacc
     4621 tgcagacggc tcaggacacg gataacggct actccgtgtt tgagcagtca ctgttgcggt
     4681 atatcgctgc cgggctgggt gtctcgtatg agcagctttc ccggaattac gcccagatga
     4741 gctactccac ggcacgggcc agtgcgaacg agtcgtgggc gtactttatg gggcggcgaa
     4801 aattcgtcgc atcccgtcag gcgagccaga tgtttctgtg ctggctggaa gaggccatcg
     4861 ttcgccgcgt ggtgacgtta ccttcaaaag cgcgtttcag ctttcaggaa gcccgcagcg
     4921 cctgggggaa ctgtgactgg ataggctccg gtcgtatggc catcgatggt ctgaaagaag
     4981 tacaggaagc ggtgatgctg atagaagccg gactgagcac ctacgagaaa gagtgcgcga
     5041 aacgcggtga cgactatcag gaaatttttg cccagcaggt ccgtgaaacg atggagcgcc
     5101 gcgcggctgg tcttaaaccg cccgcctggg cggctgcggc atttgaatcc gggctgcgac
     5161 aatcaacaga ggaggagaag agtgacagca gagctgcgta atctcccgca tattgccagc
     5221 atggccttta atgagccgct gatgcttgaa cccgcctatg cgcgggtttt cttttgtgcg
     5281 cttgcaggcc agcttgggat cagccgcctg acagatgcgg tgtccggtga cagcctgact
     5341 gccggagagg cacccgcgac gctggcgtta tccggtgatg atgacggacc acgacaggcc
     5401 cgcagttatc aggtcatgaa cggcatcgcc gtgctgccgg tttccggcac gctggtcagc
     5461 cggacgcggg cgctgcagcc gtattcgggg atgaccggtt acaacggcat tatcgcccgt
     5521 ctgcaacagg ctgccagcga tccgatggtg gacggcattc tgctcgatat ggacacgccc
     5581 ggcgggatgg tggcgggggc atttgactgc gctgacatca tcgcccgtgt gcgtgacata
     5641 aaaccggtat gggcgcttgc caacgacatg aactgcagtg caggtcagtt gcttgccagt
     5701 gccgcttccc ggcgtctggt cacgcagacc gcccggacag gctccatcgg cgtcatgatg
     5761 gctcacagca attacggtgc tgcgctggag aaacagggcg tggaaatcac gctgatttac
     5821 agcggcagcc ataaggtgga tggcaacccc tacagccatc ttccggatga cgtccgggaa
     5881 acactgcagt cccggatgga tgcaacccgc cagatgtttg cgcagaaagt gtcggcatat
     5941 accggcctgt ccgtgcaggc tgttctggat accgaggctg cagtgtacag cggtcaggag
     6001 gccattgatg ccggactggc tgatgaactt gttaacagca ccgatgcgat caccgtcatg
     6061 cgtgatgcac tggatgcacg taaatcccgt ctctcaggag ggcgaatgac caaagagact
     6121 caatcaacaa ctgtttcagc cactgctttg caggctgacg ttactgacgt ggtgtcagcg
     6181 acggagggcg agaacgccag cgcggcgcag ccggacgtga acgcgcagat caccgcagcg
     6241 gttgcggcag aaaacagtcg cattatgggg atcctcaact gtgaggaggc tcacggacgc
     6301 gaagaacagg cacgcgtgct ggccggaacc cccggtatga ccgtggaaac ggcccgccgc
     6361 attctggcag ctgcaccaca gagtgcacag gcgcgcagtg acactgcgct ggatcgtctg
     6421 atgcagggtg caccggcacc gctggctgca ggtaacccgg catctgatgc cgttaacgat
     6481 ttgctgaaca caccagtgta agggatgttt atgacgagca aagaaacctt tacccattac
     6541 cagccgctgg gcaacagtga cccggctcat accgcaaccg cgcccggcgg attgagtgcg
     6601 aaagcgcctg caatgacccc gctgatgccg gatacctcca cccgtaagct ggttgcgtgg
     6661 gatggcacca ccgacggtgc tgccgttggc atcctagcgg ttgctgctga ccagaccagc
     6721 accacgctga cgttctacaa gtcaggcacg ttccgttatg aggatgtgct ctggccggag
     6781 gctgccaccg acgagacgaa aaaacggacc gcgtttgccg gaacggcaat cagcatcgtt
     6841 taaccttacc cttcatcact aaaggccgcc tgtgcggctt tttttacggg atttttttat
     6901 gtcgatgtac acaaccgccc agctgctggc ggcaaatgag cagaaattta agtttgatcc
     6961 gctgtttctg cgtctttttt tccgtgagag ctatcccttc accacggaga aagtctatct
     7021 ctcacaaatt ccgggactgg taaacatggc gctgtacgtt tcgccgattg tttccggtga
     7081 ggttatccgc tcccgtggcg gctccacctc tgaatttacg ccgggatatg tcaaacccaa
     7141 gcatgaggtg aatccgcaga tgatcctgcg tcgcctgccg gatgaagatc cacagaatct
     7201 ggcagaccct gcttaccgcc gccgtcgcat catcctgcag aacatgcgcg atgaggagct
     7261 tgccattgcc caggtcgaag agatgcaggc cgtttctgcc gtgctcaagg gcaaatacac
     7321 catgaccggt gaagccttcg atccggttga ggtggatatg ggccgcagtg cggcgaataa
     7381 catcacgcag tccggcggca cggagtggag caagcgtgac aagtccacgt atgacccgac
     7441 cgacgatatc gaagcctacg cgctgaacgc cagcggtgtg gtgaatatca tcgtgttcga
     7501 tccgaaaggc tgggcgctgt tccgttcctt caaagccgtc aaggagaagc tggatacccg
     7561 tcgcggctct aattccgagc tggagacagc ggtaaaagac ctgggcgaag cggtgtccta
     7621 taaggggatg tatggcgata cggcgatcgt cgtgtattcc ggacagtacg tggaaaacga
     7681 cgtcaaaaag aacttccttc cggacaacac gatggtgctg gggaacactc aggcacgcgg
     7741 tctgcgcacc tatggctgca ttcaggatgc ggacgcacag cgcgaaggta ttaacgcctc
     7801 tgcccgctac ccgaaaaact gggtgaccac cggcgatccg gcgcgtgagt tcaccatgat
     7861 tcagtcagca ccgctgatgc tgctggctga ccctgatgcg ttcgtgtccg tacaactggc
     7921 gtaatcatgg cccttcgggg ccattttctc tctgtggagg agtccatgac gaaagatgaa
     7981 ctgatttccc gtcttcaggt gctgggtgag caactgaacc gtgatgtcag cctgacgggg
     8041 acgaaagaag aactggcgct ccgtgtggca gagctggaag aggagcttga tgacacggat
     8101 gacgctgccg gtcaggacac gtctgtcagc ccggaaaatg cgctgaccgg gcatgaaaat
     8161 gaggtggtgt cagcgcagcc gggtaccgtg actgatacgg ctgatctggt cacggttgtg
     8221 gcactggtga cgctgcatac tgatgcactt cacgccacgc gggatgaggc tgtggcattt
     8281 gtgctgccgg gaacggcgtt ccgtgtctct gccggtgtgg caactgaaat gacagagcgc
     8341 ggcctggcca gaatgcaata acgggaggcg ctgtggctga tttcgataac ctgttcgatg
     8401 ctgccattgc ccgcgccgat gaaacgatac gcgggtacat gggaacgtca gccaccatga
     8461 catccggtga gcagtccggt gctgtgatac gtggtgtttt tgatgaccct gaaaatatca
     8521 gctatgccgg acagggcgtg cgcgttgaag gctccagccc gtccctgttt gtccggactg
     8581 atgatgtgcg gcagctgcgg cgcggcgaca cgctgactat cggtgaggaa aacttctgga
     8641 tagaccggat ttcgccggat gatggcggaa gctgtcatct ctggcttggg cggggcgtac
     8701 cgcctgccgt taaccgtcgc cgctgaaagg gggatgtatg gccataaaag gtcttgagca
     8761 ggccgttgaa aacctcagcc gtatcagcag aacggcggtg cccggtgccg ccgcaatggc
     8821 tattaaccgc gttgcttcat ccgcgatatc gcagtcggcg tcacaggttg cccgtgagac
     8881 aaaggtacgc cggaaactgg taaaggaaag ggccaggctg aaaagggcca cggtcaaaaa
     8941 tccgcaggcc agaatcaagg ttaaccgggg ggatttgccc gtaatcaagc tgggtaacgc
     9001 gcggattgtc ctgtcccgac gcaggcgtcg taaaaagggg cagcgttcat ccctgaaagg
     9061 tggcggcagc gtgcttgtgg tgggtaaccg tcgtattccc ggcgcgttta ttcagcaact
     9121 gaaaaatggc cggtggcatg tcatgcagcg tgtggccggg aaaaaccgtt accccattga
     9181 tgtagtgaaa atcccgatgg cggtgccgct gaccacggcg tttaaacaaa atattgagcg
     9241 gatacggcgt gaacgtcttc cgaaagagct gggctatgcg ctgcagcatc aactgaggat
     9301 ggtaataaag cgatgaaaca tactgaactc cgtgcagccg tactggatgc actggagaag
     9361 catgacaccg gggcgacgct ttttgatggt cgccccgctg tttttgatga ggcggatttt
     9421 ccggcaattg ccgtttatct caccggcgct gaatacacgg gcgaagagct ggacagcgat
     9481 acctggcagg cggagctgca tattgaaatt ttcctgcctg ctcaggtgcc ggattcagag
     9541 ctggattcgt ggatggagtc ccggatttat ccggtgatga gcgatatccc ggcactgtca
     9601 gatttgatca ccagtatggt ggccagcggc tatgactacc ggcgcgacga tgatgcgggc
     9661 ctgtggagtt cagccgatct gacttatgtc attacctatg aaatgtgagg acgctatgcc
     9721 tgtaccaaat cctacaatgc cggtgaaagg tgccgggacc accctgtggg tttataaggg
     9781 gagcggtgac ccttatgcga atccgctttc agacgttgac tggtcgcgtc tggcaaaagt
     9841 taaagacctg acgcccggcg aactgaccgc tgagtcctat gacgacagct atctcgatga
     9901 tgaagatgca gactggactg cgaccgggca ggggcagaaa tctgccggag ataccagctt
     9961 cacgctggcg tggatgcccg gagagcaggg gcagcaggcg ctgctggcgt ggtttaatga
    10021 aggtgatacc cgtgcctata aaatccgctt cccgaatggc acggtcgatg tgttccgtgg
    10081 ctgggtcagc agtatcggta aggcggtgac ggcgaaggaa gtgatcaccc gcacggtgaa
    10141 agtcaccaat gtgggacgtc cgtcgatggc agaagatcgc agcacggtaa cagcggcaac
    10201 cggcatgacc gtgacgcctg ccagcacctc ggtggtgaaa gggcagagca ccacgctgac
    10261 cgtggcattc cagccggagg gcgcaactga caagagcttc cgtgcggtgt cagcggataa
    10321 aacaaaagcc accgtgtcgg tcagtggtat gaccatcacc gtgaacggcg ttgctgcagg
    10381 taaggtcaac attccggttg tatccggtaa tggtgaactt gctgcggttg cagaaatcac
    10441 tgtcaccgac agttaatccg gagagtcagc gatgttcctg aaaaccgaat catttgaaca
    10501 taacggcgtg accgtcacgc tttctgaact gtcagccctg cagcgtattg agcatctcgc
    10561 cctgatgaaa cggcaggcag aacaggcgga gtcagacagc aaccggaagt ttactgtgga
    10621 agacgccatc agaaccggcg cgtttctggt ggcgatgtcc ctgtggcata accatccgca
    10681 gaagacgcag atgccgtcca tgaatgaagc cgttaaacag attgagcagg aagtgcttac
    10741 cacctggccc acggaggcaa tttctcatgc tgaaaacgtg gtgtaccggc tgtctggtat
    10801 gtatgagttt gttgtgaata atgcccctga acagacagag aacgccgggc ctgcagagcc
    10861 tgtttctgcg ggaaagtgtt cgacggtgag ctgagttttg ccctgaaact ggcgcgtgag
    10921 atggggcgac ccgactggcg tgccatgctt gccgggatgt catccacgga gtatgccgac
    10981 tggcaccgct tttacagtac ccattatttt catgatgttc tgttggatat gcacttttcc
    11041 gggctgacgt acaccgtgct cagcctgttt ttcagcgatc cggatatgca tccgctggat
    11101 ttcagtctgc tgaaccggcg cgaggctgac gaagagcctg aagatgatgt gctgatgcag
    11161 aaagcggcag ggcttgccgg aggcgttcgt tttggcccgg acgggaatga agttatcccc
    11221 gcttccccgg atg
//
LOCUS       DAZFJL010000147        11179 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZFJL010000147 DAZFJL010000000
VERSION     DAZFJL010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.149"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(30..851)
                     /gene="dapB"
                     /locus_tag="ACHJ8C_003943"
     CDS             complement(30..851)
                     /gene="dapB"
                     /locus_tag="ACHJ8C_003943"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO5553724.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(1012..1377)
                     /locus_tag="ACHJ8C_003944"
     CDS             complement(1012..1377)
                     /locus_tag="ACHJ8C_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO5553725.1"
                     /translation="MTYGEAYLDGWKNIFNYEGVSNRFEFWSFMIGSGVICLLPLLCW
                     WLAVTINNDYGVFIFFALPASFILTLIFAVPAIALAVRRMHDIGYSGWWVTIAVLIPV
                     TGVILLILCCLPSKSQDQA"
     gene            complement(1400..2314)
                     /gene="rihC"
                     /locus_tag="ACHJ8C_003945"
     CDS             complement(1400..2314)
                     /gene="rihC"
                     /locus_tag="ACHJ8C_003945"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO5553726.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNVDIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFALKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAL"
     gene            complement(2380..3330)
                     /gene="ispH"
                     /locus_tag="ACHJ8C_003946"
                     /gene_synonym="lytB"
     CDS             complement(2380..3330)
                     /gene="ispH"
                     /locus_tag="ACHJ8C_003946"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO5553727.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKGVNCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(3332..3781)
                     /gene="fkpB"
                     /locus_tag="ACHJ8C_003947"
     CDS             complement(3332..3781)
                     /gene="fkpB"
                     /locus_tag="ACHJ8C_003947"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO5553728.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(3889..4383)
                     /gene="lspA"
                     /locus_tag="ACHJ8C_003948"
     CDS             complement(3889..4383)
                     /gene="lspA"
                     /locus_tag="ACHJ8C_003948"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO5553729.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(4383..7199)
                     /gene="ileS"
                     /locus_tag="ACHJ8C_003949"
     CDS             complement(4383..7199)
                     /gene="ileS"
                     /locus_tag="ACHJ8C_003949"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO5553730.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIIKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKTKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVSDYTILGTVKGAELELLRFTHPFMDFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVIALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(7242..8183)
                     /gene="ribF"
                     /locus_tag="ACHJ8C_003950"
     CDS             complement(7242..8183)
                     /gene="ribF"
                     /locus_tag="ACHJ8C_003950"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO5553731.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(8191..8409)
                     /locus_tag="ACHJ8C_003951"
     CDS             complement(8191..8409)
                     /locus_tag="ACHJ8C_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO5553732.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSARKIAAGGQFLPMICAIAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            8512..8775
                     /gene="rpsT"
                     /locus_tag="ACHJ8C_003952"
     CDS             8512..8775
                     /gene="rpsT"
                     /locus_tag="ACHJ8C_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO5553733.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            complement(8871..9776)
                     /gene="nhaR"
                     /locus_tag="ACHJ8C_003953"
     CDS             complement(8871..9776)
                     /gene="nhaR"
                     /locus_tag="ACHJ8C_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO5553734.1"
                     /translation="MSMSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRAL
                     EERLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVA
                     DALSKRLVSSVLDAAVVEDEQIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQE
                     GLFSMKIGECGVSFWCTNPLPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNV
                     EILGEFDDAALMKAFGATHNAIFVAPSLYANDFYNDDSVVEIGRVENVMEEYHAIFAE
                     RMIQHPAVQRICNTDYSALFTPASK"
     gene            complement(9836..11002)
                     /gene="nhaA"
                     /locus_tag="ACHJ8C_003954"
     CDS             complement(9836..11002)
                     /gene="nhaA"
                     /locus_tag="ACHJ8C_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO5553735.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAVLAMIMANSGATSGWYHDFLET
                     PVQLRVGTLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         2837 a         3119 c         2678 g         2545 t
ORIGIN      
        1 atttttgcat cacaataaat atcttgtggt tacagattat tgagatcaag cacatctcgc
       61 atatcaaaaa gaccactttc cttaccactc aaccacaaag ccgatcttac cgcaccgtta
      121 gcaaatgtca tgcggctgga tgccttatgg gtgatctcca gacgctcgcc aatatcggca
      181 aacatcgcgg tatgttcgcc aacgatgtca cctgcacgca cggtggcaaa accaatggtg
      241 ccaggcacac gttcaccggt atggccttca cgactgtaga ccgcgcaatc tttcagatct
      301 ttatcaagag cgtgggcgat cgcctctccc attgccagtg cggtgcctga cggcgcatca
      361 actttatgtc tatgatgtgc ttcaataatt tcgatatcgg tgtagtcccc catcactttg
      421 gctgctttct ccagcagttt aagcatgacg ttaacgccaa cgctaaaatt ggcggcaaag
      481 acaatcgcaa tatcggcggc ggcgtcacga atcgcctgtt taccggcttc gtcaaacccc
      541 gtagtgccga tcaccatccc tttgccatgc tggcgacaaa aagcgagatg gttcagcgta
      601 ccttccggac gggtaaaatc gataaacaca tcaaaatcat cttttatcgc atcaagacta
      661 ctttgcacag taacgcctgt tttcccggct ccggccagtt cgcccgcatc gctgcccagt
      721 aaagaagatc cttcgcgctc cagcgccgcg cccagctgca cgccttctaa tgccagcgcc
      781 gcctgaatca actggcggcc catacggccc ccggctcccg cgatggcaac gcggatgttt
      841 gcatcatgca tagctattct cttttgttaa tttgcataga ccgttttcag agtaaccagc
      901 gcatcgctac gccgccagcc aaaaacgctg ataattagaa attaatgata aacagggagg
      961 gatatcagtg aaaggcatga cgcagaaagt gatgcgtcat accctctggc attatgcctg
     1021 gtcttgtgac tttgacggca ggcagcacag tataagcaga attacgcccg taacagggat
     1081 caacacggca atggtaaccc accaccctga atatccgata tcgtgcatcc ttctgacggc
     1141 taacgctatc gccggtacgg caaaaatcaa cgttaaaata aatgacgcgg gcagcgcaaa
     1201 aaaaatgaaa acaccatagt cattgttaat tgttaccgct aaccaccaac ataaaagcgg
     1261 aagcagacag ataacgccac taccaatcat aaacgaccag aactcgaagc gattggaaac
     1321 accttcataa ttaaagatgt ttttccaccc gtccagatac gcttctccat acgtcataga
     1381 acacttcctt gttttattat tagagcgcca gggccagcac ctcagccacc cactgctgga
     1441 agcctttcac atccagatcc agcgccacct gtacattggc tggtttgccc aggcaaccgt
     1501 cgatatcaac aaccgtcgtg cctgaggtaa attcaccctg agtttctacc gcgacaaaac
     1561 acggtttgag agcgaacaat tccgggcgca ccagccaggc gatagcgcaa agatcgtgca
     1621 tacgtagtcc actttgcata ctgccgctac ggtagtggct aaacagggcg tgtagcattt
     1681 ttccggtgcg attcaattcc ggcaaagtag cgagatagtc aggggttaat attgcctgat
     1741 tggtgacatc cagaccgcac atgacgattt cgatgccgct acggaagaca caggctgccg
     1801 cttctggatc ggcagcgata ttaaactcgg cgtttggtgt gcagttgccg cgaccagcag
     1861 aaccgcccat gatcaccaga cggcgaatat acggcttaca ttccgggcat tgtgaaagta
     1921 acagcgcaat attggttaac gggccgatgg ccaccagggt aacgggctct ggcgcacgca
     1981 tcagggcatc acgaattgcc agaaacgccg gtatacccag tggcttacgg ttgtgttcaa
     2041 caaagtcata gccagccatt cccgattcac cgtgtacaga tgccgcatcg cgcggcgcac
     2101 gaaccagtgg tacggcagcg ccctgggcaa gcggaatatc cacattccag aaatgcagca
     2161 gttgcagggc gttgcgggta gttttctcaa ccgagacatt acccgcgacg gtggtcatca
     2221 gttgcaggtc gagctcgggg gcaaaaatcg cggcggcaat ggcgacggca tcgtcaatgc
     2281 cggggtctgt atcgaggaag ataggtaaac gcatgttttc tccataaaaa atgccggtaa
     2341 caagaccggc attttcgcat aacttaggct gctaatgact taatcgactt cacgaatatc
     2401 gacacgcagc tctttcggca cttcgaaaac aatgttttct tcacgacctt ccagcggaat
     2461 ggcttcacca ccgcccagct gttgcaaacg tgccaccaca ttctgcacca gaatatccgg
     2521 agccgatgcg cccgcagtca cgccaacgca gttcacgcct tttacccact cttcctggat
     2581 atctttcgca tcgtcaatca aaaatgcgcg tttgcccata cgctgggcca gttccgccag
     2641 acggttggag ttggaagagt ttttcgaacc aaccaccagt actacttccg cctgctctgc
     2701 cagggcgcgt accgcttcct gacggttagt cgtggcgtag cagatgtcat ctttgcgcgg
     2761 accgacaatt ttcgggaagc gtttacgcag cgcgtcgatc acatcagacg tgtcatccac
     2821 cgacagcgtg gtctgggtca taaaggagag cttctcttcg tttttgaccg tcagtttcca
     2881 cacatcgtct ggcgattcga ccagatacat tcccccttcc gggttgctgt actggcccat
     2941 cgtcccttcc acttccgggt gcccggcgtg accgatgaga atagattctt caccacgacg
     3001 gctggcgcgg gcgacttcca tatgcacttt ggtcaccagc ggacaggtgg catcaaacac
     3061 cgtcaaatcg cggcttttcg cttcgttacg tactgcctga gaaacaccgt gtgcggagaa
     3121 aatcaggatc gcgccgtccg gtacttcgct aatctgctca ataaagatag ccccacgctc
     3181 gcgcaggcta tcgaccacgt agcggttatg caccacttcg tgacggacat atatcggtgc
     3241 gccgtaaatg gccagcgcgt tttcaacaat gctgatagcg cggtctaccc cggcacaaaa
     3301 accacgcggg ttggccaaca ggatctgcat gttacgcctc cagtgccgga tcgatttcca
     3361 gcacttcaat atcaaaatga acggtctgcc cggccagcgg atggttgaaa tcaacggtaa
     3421 tggagtcgcc gttaatttcg cggatcacgc caggcatctc actgccatcc attgcggtaa
     3481 aaagcatgat tgcgccaatt tctggctcgc ctgcatccat aaattcacgg cgggagaagt
     3541 actgaatcag gtccggtgac ggcacgccaa acgccgcatc tggctccagc gagaaggtgg
     3601 ttttatcgcc cactttcagc cccagcaggt gttgctccag cccttcagaa agagaagcat
     3661 cacccaggcg gaacagcgcc ggtttaccgt tgttgcgggt agactcagcg gtggtgccat
     3721 cgtcgagttt tagcgtgaag tgcaccagga cggcgctatt gctctgtaca gattcagaca
     3781 tgcaggtttg ctcttatatt ttgaggattt ttgccggatg cgacctacga tttcgcagca
     3841 atctgtaggc cggataagat gcgtcagcat cgcatccggc agggtttatt attgtttttt
     3901 cgctttagaa ggtaaaaaac cttccagcac aatcagtgcc gcaccgacac aaatggcagt
     3961 atcggcaagg ttgaaggtgg cgaagtgcca gtcgccgaca tagaagtcga tcatatcgac
     4021 aacgaagccg tgccacaggc ggtcgaacag gttgcccagt gcgccgccaa taatcagcgc
     4081 gtaagcgata ttgtttagct tctgcgtggc cttcgagcga tacatcatca ctgccaggat
     4141 cacgctaata ccaatcgcaa taccggcaaa gaaccaacgc tgccagccgc cgctatcggc
     4201 aaggaaacta aacgctgcgc catagttacg cgcataatgc agattaagcg acgggaacag
     4261 cgggaccgta tcccccagag caaagttctg gaggatcagg tatttgctgc ccagatcgat
     4321 aatcagcacg actaccacca gccacagcca gcgtagccct gttgaacaga tcgattgact
     4381 catcaggcaa acttacgttt ttcaccgtca ccggcgacgt tgctgacaca gcggccgcag
     4441 atttctgcgt gttccgccac cttgccgaca tcctgggtgt agtgccagca gcgagggcac
     4501 ttctcacctt cggctttact caacgcgact ttcagccctt tgaggacttc gctctgctgg
     4561 gcatcagcag gtgcgtcgtt atagtctgca acggtagcgc cggaggtcaa caggacaaat
     4621 cgtaattcat cgcccagcgc ggtcagtttc gccgccagtt ccggttctgc atacaaggtt
     4681 actgccgctt ccagcgagcc gcccactttc ttatcggcac gcgcttgctc aatgactttg
     4741 ttcacttcgc cacgcacttt caacagctcg tcccagaacg catcgttcat tgcttcactg
     4801 tctgccagac caaacaggcc ttcgtaccac tcgccggtga agacgtattt ttcacgttcg
     4861 cccggcaggt aaccccacac ttcatcagcg gtgaaggaga ggattggtgc catccagcga
     4921 accagcgctt ctgcgatgtg atacagcgca gtctggcagc tacgacgcgc cacgctgtcc
     4981 gctttggcgg tatactgacg gtctttgatg atgtcgaggt agaaggaacc catctcaacg
     5041 gagcagaagc gcatcagacg ctgtaccact tcgtggaaat cgtatgcttc gtacgccttg
     5101 aggatgtctt cctgtgccgc tttcgcacaa cctacggccc agcgatccag taccaccatc
     5161 tcttccggtt tcaccatatc ttttgccgga tcaaaaccgt tcaggtttgc cagcaggaag
     5221 cgcgcggtgt tacggatacg acgatagctg tcggcagcac gtttcaggat ctcgtcagaa
     5281 acggccattt cgccagtgta gtcggttgat gccacccaca gacgcagaat atccgcaccc
     5341 agtttgttca tcacatcctg cggcgaaacg gtgttaccga tggatttaga catcttgcga
     5401 ccctgaccat ccacggtaaa gccgtgagtc agtacctgac gatatggcgc tttgcccttc
     5461 atcgcggtag agatcatcag ggaagacatg aaccagccac ggtgttggtc agaaccttcc
     5521 agatacatgt ccgctgcgtg accggcaaat tccggacgca catcaacaac ggaagagtgg
     5581 gtagatccgg agtcaaacca tacatccagc gtatccggta ctttcacata ctggtcagct
     5641 tcgtcgccga ggatctcttt cgcatcgaga tcccaccacg cctggatgcc gtcaacttca
     5701 acgcgttttg ccacttcttc catcagttcg agagtacgcg gatgcagttc ttctgtgtct
     5761 ttgtgcacga acagtgacat cggcacgccc caggtacgct gacgagagat acaccagtca
     5821 ggacggttag caaccatcga ctcgatacgc gcctggcccc agtcagggat ccactgcacg
     5881 cctttgatct ctttcagtga ctgcgcacgc agacctttct gatccatgct gacgaaccac
     5941 tgcggcgtcg cgcggaagat gatcggcgtt ttatgacgcc agcagcacgg atagctgtgt
     6001 tgcattttct caacgtgcaa cagtgcgcct ttttcctgca acaacgcaat gacaatatcg
     6061 ttcgctttga agacgttaac gccatccaga gtcggataag tacccggcag ataagtgccg
     6121 tccgggccaa ccgggttagc ggtttccaga ccatattttt gaccgatcac atagtcgtcc
     6181 ggaccgtggc ctggcgcggt atgaacggca ccggtaccgg catccagcgt aacgtggtcg
     6241 ccgagaattg ccggaacatc gaagtccata aacggatggg taaagcgcaa cagttccagc
     6301 tcggcacctt tcaccgtgcc aagaatggtg taatcgctaa cgccgatacg ctgcattacg
     6361 ctttcaacca gatctttcgc gaggatcacg gcctgaccgt cgatttgcac cagcgcataa
     6421 tcaaaatcag gtgcaatgga gattgcgcgg ttagcaggca gcgtccacgg cgtggtggtc
     6481 catataacca gcgaaattgg gccgttaaca ttgcttacgc caaatttcgt tttaagcgca
     6541 tcctgatcga ccgcctggaa agcgacgtcg atggacggag aagttttgtc gtaatactca
     6601 acttccgctt ctgccagtgc agaacggcag tcaacgcacc agtgcaccgg cttcgcgcct
     6661 ttgtgcaggt gaccgttgcc gatgattttg cccagcgcgc ggatgatgtt ggcttcagtt
     6721 ttgaagtcca tggtcaggta cgggtgcgac cagtcgccca gcacgcccag acggataaag
     6781 tctttgcgct gaccgtcaac ctgggtcgca gcgtattcgc ggcacttggc gcggaactca
     6841 gcggcggtga atttctcccc cggcttaccg tattcttgct ctactttcag ttcgattggc
     6901 agaccatgac agtcccagcc aggcacatac ggcgagtcat atccagaaag ccctttggac
     6961 ttaatgataa tgtctttcag aatcttgtta accgagtgac caatatgaat gctgccattc
     7021 gcataaggag ggccatcatg cagaatgaag gtttttttgc cttttttagc tgcacgaatg
     7081 atgccgtaca gatcatcatc agtccaacgc gccagcattc ccggttcgcg cttggcgaga
     7141 tcgccacgca tcgggaaccc tgtttccggc aaattcaggg ttgatttata gtcactcatc
     7201 agattctcgg ttccgtattt cggtttgatt acataacagg cttaagccgg ttttgttagc
     7261 ccaaaaaatt cgcgggcggt taattcatca cgcgcaatct gcgctttcag ttcgtccagc
     7321 gatgcaaatc gctgctcatt gcgtattttt ttacgcagca ctacttgtat atggcgacca
     7381 taaaggtcca ttgcaacatc taacaaatgc acttccagtt gctggcgaat accggcaacc
     7441 gttgggcgtg ttccgatgtt tgcaacgccg ggtaacggct tttcgccaag gcccaacact
     7501 tctaccgcat aaaccccttt caccggggaa acctgacggc gtagcggtac attcgccgtc
     7561 gggaaaccta tagtgcgccc taattcatca ccgtggacta cacgcccgga gatggcaaac
     7621 gggtgcccca gtaaactttc tgccagagcc agattgtcat ccgcaagcgc ctgacgcacg
     7681 gcggtgctgc tgatacgcac gccaccttcg caaaaagttt gcgtgctggt gatatcgaag
     7741 ccgtattcca tgcccgcttt ctgtaataac aagaaatcgc cttcacgacc agcgccaaag
     7801 cggaaatcgt cacctacggc aagaaatttt acccgcaagt gcttcaccag aagatcactg
     7861 atgaagtttt gcgcggttaa cgccgcaaaa cgcctgtcaa aacgcacgca cagcacgtaa
     7921 tcaacaccac actctgcaag ataacgcagt ttttcccgca gccgggtgag ccgtgccggg
     7981 gctttatcag tagcaaacag ttccagtggt tgaggttcaa aaagcatcac catcaccggt
     8041 aagttgcgct tgcgcccttc ttcctgcaag ccctgtaaca gcgcgcgatg accgcgatgc
     8101 acgccgtcga aattaccaat agtcagcaca cacccttctt gcggggcctg gctgagatta
     8161 tgtatgccgc gtatcagctt catgtctggc tcaaaacagt gaaaatcgtc cgagtatacc
     8221 ttgtacagcg gtcaaggtta accggcgatt gagtaccgaa atcagaaaga agtggctttt
     8281 cattgctatg gcgcaaatca tgggaagaaa ctgaccgcct gctgcaattt ttctcgcgga
     8341 aaagctgtat tcacaccccg caagctggta gaatcctgcg ccatcactac gtaacgagtg
     8401 ccggcacatt aacggcgctt atttgcacaa atccattgac aaaagaaggc taaaagggca
     8461 tattcctcgg cctttgaatt gtccatatag aacacatttg ggagttggac cttggctaat
     8521 atcaaatcag ctaagaagcg cgccattcag tctgaaaagg ctcgtaagca caacgcaagc
     8581 cgtcgctcta tgatgcgtac tttcatcaag aaagtatacg cagctatcga agctggcgac
     8641 aaagctgctg cacagaaagc atttaacgaa atgcaaccaa tcgtggaccg tcaggctgct
     8701 aaaggtctga tccacaaaaa caaagctgca cgtcataagg ctaacctgac tgcacagatc
     8761 aacaaactgg cttaattgcc tgttgttgtc agctttgtaa aaaaacccgc tctggcgggt
     8821 ttttttatat ccatctgcag gccaaaattc ggcctgcaaa gtacaaatgt ttattttgaa
     8881 gctggagtaa acagcgcaga atagtctgta ttgcagatac gctgtactgc agggtgctga
     8941 atcatccttt cggcaaaaat cgcgtggtac tcttccatca cgttctcaac acggcctatc
     9001 tccacaaccg agtcatcgtt atagaaatca ttagcgtaaa gcgaaggtgc aacgaaaata
     9061 gcgttatgcg tcgccccaaa ggctttcatc aacgcagcat catcaaactc acccaaaatt
     9121 tcgacgttca agccctggga gttaaaccag tttaatagct tacgccccaa cattgagcga
     9181 cgccccggaa taagtaaacg acgctcttca agacaggcag gaaacggctt ttctggtagt
     9241 gggttagtgc accagaaact gacaccacat tcgccaattt tcatggaaaa cagcccttcc
     9301 tgctgagtgg aatcgatcgg acagtcagag atgatcatat ccagtttatg ctgactcaac
     9361 tgctctaaaa gcatctcgtg cgtcgattcg aaacagcgta gatggatctg ctcgtcttcc
     9421 acaactgcgg catccagaac actgctgacc agacgtttgg aaagtgcatc tgccacacca
     9481 acatcaaaca ataagttgga ctctttgcga tagttgacga tatccagcat ttcctggctt
     9541 aaggtgaaca ttttatcggc atagcgatag accagttccc ccagttcgct gggttccaga
     9601 ccacgtcctt tacgcttaaa tagtttccct tgcaggcgct cttccagcgc ccggatctgc
     9661 ccggtaatgg tttgtggtgt taaataaagc gcctccgctg cgccaaccac agaaccttct
     9721 ttgtagacat gccagaagta atacaagtgg ttgtaattga tatgagacat gctcatttct
     9781 ctccctgata acaatgaaaa gggagccgtt tatggctccc cagtacatcg tcctgtcaaa
     9841 ctgatggacg taaacgaacg cgtaaccagc tatatccaat taccgccgaa gaaattgaac
     9901 cgacaaggat acctaatttt gcccagttaa tcagttctgg atctacgcta ccaaatgcca
     9961 ggctggcaat aaagatagac atagtaaaac cgataccgca caggatacca accgccataa
    10021 tttgctggta agtcgttccc tctggcagat gtgccaattt caaacgcagc gccaaccagc
    10081 agaacagact aatacccagt ggcttgccaa tcagcaaacc agcgatgatc cctaatggca
    10141 ggatggaggt caggccatcc agcgtgacgc cttgcagtga aacgccagca ttagcaaatg
    10201 caaacagcgg taaaatcaga tacgccaccc acgggtgcaa cacatgctcc agtcgcttcg
    10261 ctggagaacg cccatgcttc tcttttaacg gaataaagaa gccgacaatg acgcccgcca
    10321 gggttgcgtg aacccccgat ttcaacaccg ctgtccacag caccacgcca accagaatat
    10381 aaacgcccgt gcgacgtaca ccacacagat tcaataccgc gagtaccgca attgctacag
    10441 ccgcgacgcc aagagaggcc atcgataagt cattagtgta gaacaatgcg atgataatga
    10501 tggccccaag atcgtcgata atagccagag ccatcaaaaa gatcttcagc gctaacggaa
    10561 cacgacttcc caacagcgcc aacacaccaa gtgcaaaggc aatgtcagtc gccgccggga
    10621 ttgcccagcc ttcgcgagtg ataggatcgg catagttaaa agccagatag agcaatgccg
    10681 ggacaatcat cccgccgatt gcggcaataa caggaaatgc cgcctggcgc agactggcca
    10741 gcgaaccttg catcagctcg cgtttaactt ccagaccaac caacaggaaa aataccgcca
    10801 tcagagcgtc attgatccat agcagcatgt tcttgttgat ctcaagtgtc ccaacccgga
    10861 gctgaaccgg cgtctcaaga aagtcgtgat accatccact ggttgcaccg ctgttggcca
    10921 taatcatcgc taatacagcg gcaataatga gaataatgcc tcccgaggca tcactgctaa
    10981 agaatcgatg cagatgtttc actttttatt tctcttcagg tgaatagatc gttttcacga
    11041 ttttaccccg cacgaatcat cgttaaaatc agattatagt gtaggaatag ctcgcttttt
    11101 acgatctatt aaaagaattg cgccatcagc gagagatgag tttttgatgc ccgaaagcgg
    11161 cggctgatga ccttaaacc
//
LOCUS       DAZFJL010000148        11172 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFJL010000148 DAZFJL010000000
VERSION     DAZFJL010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.150"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            40..1092
                     /gene="ybhH"
                     /locus_tag="ACHJ8C_003955"
     CDS             40..1092
                     /gene="ybhH"
                     /locus_tag="ACHJ8C_003955"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO5553736.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTSIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELN
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1168..2601
                     /gene="ybhI"
                     /locus_tag="ACHJ8C_003956"
     CDS             1168..2601
                     /gene="ybhI"
                     /locus_tag="ACHJ8C_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO5553737.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            2784..5045
                     /gene="ybhJ"
                     /locus_tag="ACHJ8C_003957"
     CDS             2784..5045
                     /gene="ybhJ"
                     /locus_tag="ACHJ8C_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO5553738.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSEMADVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEECEIIK
                     AGSLINFNKNRQM"
     gene            complement(5186..6469)
                     /gene="ybhC"
                     /locus_tag="ACHJ8C_003958"
     CDS             complement(5186..6469)
                     /gene="ybhC"
                     /locus_tag="ACHJ8C_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO5553739.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPSKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDKVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSVINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DSDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(6604..7674)
                     /locus_tag="ACHJ8C_003959"
     CDS             complement(6604..7674)
                     /locus_tag="ACHJ8C_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5553740.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(7652..7870)
                     /locus_tag="ACHJ8C_003960"
     CDS             complement(7652..7870)
                     /locus_tag="ACHJ8C_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO5553741.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(7910..8077)
                     /locus_tag="ACHJ8C_003961"
     CDS             complement(7910..8077)
                     /locus_tag="ACHJ8C_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553742.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADITNI
                     RIEELKEHQAA"
     gene            8320..8922
                     /locus_tag="ACHJ8C_003962"
     CDS             8320..8922
                     /locus_tag="ACHJ8C_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553743.1"
                     /translation="MSYFLRKKWMVNLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGMCIFAGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
     gene            complement(9133..9354)
                     /locus_tag="ACHJ8C_003963"
     CDS             complement(9133..9354)
                     /locus_tag="ACHJ8C_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO5553744.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(9453..9668)
                     /locus_tag="ACHJ8C_003964"
     CDS             complement(9453..9668)
                     /locus_tag="ACHJ8C_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO5553745.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            complement(9745..9936)
                     /locus_tag="ACHJ8C_003965"
     CDS             complement(9745..9936)
                     /locus_tag="ACHJ8C_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO5553746.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDLV"
     gene            complement(9909..10090)
                     /locus_tag="ACHJ8C_003966"
                     /pseudo
     CDS             complement(9909..10090)
                     /locus_tag="ACHJ8C_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
     gene            complement(10087..10767)
                     /locus_tag="ACHJ8C_003967"
     CDS             complement(10087..10767)
                     /locus_tag="ACHJ8C_003967"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO5553747.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGIQYENDARALFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDENYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            complement(10764..>11172)
                     /locus_tag="ACHJ8C_003968"
     CDS             complement(10764..>11172)
                     /locus_tag="ACHJ8C_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFO5553748.1"
                     /translation="RREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLAFGFAGIY
                     DKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLPLCSQIF
                     RRDIRASSELTQAEAMKALGFLKQKASEQKVAV"
BASE COUNT         2659 a         2652 c         2751 g         3110 t
ORIGIN      
        1 tatcgcaata agttatgttt ttaaattgag ggcattatta tgaaaaaaat accctgcgtg
       61 atgatgcgag gtggaacctc gaggggcgcg ttcctgttag cggaacatct acccgaagat
      121 caaacgcagc gcgataaaat attgatggca attatgggtt ccggtaacga tctggaaatt
      181 gacggtattg gcggcggtaa tccgctgacc agtaaagtcg ccattattag ccgctccagc
      241 gatccgcgtg cagatgtcga ttatctgttt gctcaagtta tcgtccatga gcaacgtgtc
      301 gataccacgc ctaactgcgg caatatgctg tctggcgttg gggcattcgc cattgaaaat
      361 ggtttgattg cagcgacttc gccagttacc cgcgtacgta tccgcaacgt caatacgggt
      421 acgtctatcg aagctgatgt gcaaacgcca aatggtgttg tcgagtacga gggtagtgcc
      481 agaattgacg gcgtaccggg tactgccgca ccggttgcgc tcactttcct gaatgccgcc
      541 ggaacgaaaa ccggaaaagt cttcccgacg gataatcaga ttgattattt tgacgatgtc
      601 ccggtgacct gtatcgatat ggcgatgcca gttgtcatta ttcctgctga atatctggga
      661 aaaacaggct atgaattacc ggcagagctg aatgccgaca aagcattatt agcccgcatt
      721 gaatctatcc gtctacaagc gggtaaagca atgggcttag gcgatgtcag taatatggtt
      781 atccctaaac ctgtacttat ttctccagcg cagaaaggcg gggcaattaa tgtgcgttat
      841 tttatgccgc attcttgcca tcgcgctttg gcgataaccg gtgctattgc tatttccagt
      901 agttgtgcat tggaaggcac cgtcacccga caaatcgtcc cttctgtagg atacggcaat
      961 atcaatattg aacaccccag tggtgcgctc gacgttcatt taagtaatga aggtcaggat
     1021 gccacgacgt tacgcgcatc tgttattcgg acgacaagaa aaatattttc cggtgaagtt
     1081 tatcttccct gaaaaatttt gttgtcgtga taatgacaat caataaagga attctgtatg
     1141 agtcatacag aaagaacagg atattaaatg aataaaaaat cgttatggaa gctaattctg
     1201 atattagcga tcccatgtat tattggcttt atgccagctc cggcaggatt aagcgaactg
     1261 gcgtgggtgc tttttggtat ttacctggcg gccattgtgg ggctggttat caagcctttc
     1321 ccggaacctg tcgtactgtt aattgccgtc gccgcatcga tggtagtggt gggtaactta
     1381 tcaggtgggg aatttaaaac caccgccgta ttaagcggtt actcttccgg taccacctgg
     1441 ctggtgtttt ctgcgtttac tttaagcgcc gcgtttgtaa ccacaggctt aggtaaacgt
     1501 attgcctatc tgctgattgg taaaattggt agcactaccc tgggtctggg ttacgttacg
     1561 gtatttctcg atctggtact ggctccggca acaccgtcta acaccgcgcg tgcgggcggc
     1621 atcgtgttac cgatcatcaa cagcgtggca gtggctttgg gatcagaacc ggaaaaaagt
     1681 ccgcgtcgtg ttggacatta cctgatgatg tccatttaca tggtcactaa aaccaccagc
     1741 tatatgttct ttaccgcaat ggcggggaac attctggcgc tgaaaatgat caacgacatt
     1801 ctgcacctgc aaattagctg gggtggatgg gcgctagccg ccggattgcc tggcatcatt
     1861 atgctgctgg tcaccccgct ggtgatttac accatgtatc caccagaaat taagaaggtg
     1921 gataacaaaa ccatcgctaa agcgggcctt gccgaactgg gaccgatgaa aatccgcgaa
     1981 aaaatgctgc tcggtgtctt tgtgctggcg ctgctgggct ggattttcag taagtctctg
     2041 ggggttgatg aatccaccgt ggcaatcgtt gttatggcaa ctatgctgct gctgggtatc
     2101 gttacctggg aagacgtggt taaaaataaa ggcggctgga ataccttaat ctggtacggc
     2161 ggtattatcg gcttaagctc cttattatcg aaagttaaat tcttcgaatg gttagctgaa
     2221 gtctttaaaa ataacctggc atttgatggt cacggtaacg ttgctttctt cgttattatt
     2281 ttcctcagca tcatcgtgcg ttatttcttc gcttctggta gtgcctatat cgttgctatg
     2341 ttacctgtgt ttgccatgct ggcgaatgtc tccggcgcac cgttaatgtt aaccgcgctg
     2401 gcactgttgt tctctaactc ctatggcggc atggttactc actatggcgg cgcggcaggt
     2461 ccggtcatct ttggcgtggg ttacaacgat attaaatcct ggtggttggt cggtgcggta
     2521 ctgacgatat taaccttcct ggtgcatatc accctcggcg tgtggtggtg gaatatgctg
     2581 atcggctgga acatgctgta aatatactcg tcatacttca agttgcatgt gctgcgtctg
     2641 cgttcgctca ccccagtcac ttacttatgt aagctcctgg ggattcactc gcttgtcgcc
     2701 ttcctgcaac tcgaattatt tagagtatat ccatttatta tctttctgcg cacttcacgg
     2761 tgcgcagata tctggagcat ttgatgatca agttatctga aaaaggcgtg tttctcgcca
     2821 gcaataacga aataattgcc gaagaacatt tcaccggcga aataaaaaaa gaagaagcca
     2881 aaaaaggcac tattgcctgg tctattctct cttctcataa tacgtccgga aatatggata
     2941 aacttaaaat taagtttgat tcattagcct ctcacgatat tacctttgtt ggtattgtac
     3001 agaccgctaa agcgtccggt atggaacgtt tcccgctgcc gtatgtgctg accaactgcc
     3061 ataactcact ctgcgccgtc ggcggcacca ttaacggtga tgaccatgtt tttggattat
     3121 cggcggccca gcgttatggc ggtatttttg tgccaccgca tattgcggtc atccatcaat
     3181 atatgcgtga aatgatggcg ggcggcggca aaatgatcct cgggtcagac agccataccc
     3241 gttacggtgc gttagggaca atggcggttg gcgagggcgg cggcgagttg gtaaaacagc
     3301 tgcttaatga cacctgggat atcgactatc cgggcgtggt tgcggtgcat ctgaccggaa
     3361 aacctgcgcc gtatgtgggg ccgcaggatg tggcgctggc tatcatcggt gccgtgttca
     3421 aaaacggtta cgtcaaaaac aaagtgatgg agttcgtagg tcccggtgtt gcagcgctct
     3481 ctaccgattt ccgcaacagc gttgacgtga tgaccactga aacgacctgt ttaagttctg
     3541 tctggcaaac cgatgaagaa gtccataact ggctggcgct gcacggtcgc ggccaggatt
     3601 actgccaact taaccctcaa ccaatggcgt actacgatgg ctgcatcagc gttgatttaa
     3661 gcgccatcaa accaatgatt gcgctgccat tccacccgag caacgtgtat gaaatcgaca
     3721 cgctgaacca gaacctgacc gacattctgc gtgagattga aattgagtcc gaacgcgtgg
     3781 cgcacggtaa agccaaactc tcgctgctgg ataaagtgga aaatggtcgc ctgaaagtgc
     3841 agcaggggat tatcgcgggc tgttctggcg gtaactatga aaacgtcatc gcggcggcga
     3901 atgcgctgcg cggtcaatcc tgcggcaatg acaccttctc gttagcggtt tacccgtcat
     3961 cacagccggt gtttatggat ctcgccaaaa aaggtgtggt agcagatttg attggcgcag
     4021 gcgcaatcat cagaaccgcg ttctgcggcc catgctttgg cgcgggcgat acgccaatca
     4081 acaacggttt gagtattcgc cacaccacgc gtaacttccc gaaccgcgaa ggctctaagc
     4141 cagctaatgg gcagatgtca gcggtggcat tgatggacgc tcgttctatc gctgcgactg
     4201 cggcaaacgg tggctattta acctctgcca gcgaactcga ttgctgggac aacgtgccgg
     4261 agtacgcctt cgatgtaacg ccgtataaaa accgtgttta tcagggcttt gtgaaagggg
     4321 cgacccagca accgctgatt tacggaccga acattaaaga ctggccagaa ttgggtgcgc
     4381 tgactgacaa tatcgtcctc aaagtgtgct cgaagatcct cgacgaagtg accaccaccg
     4441 acgaactgat tccttccggt gaaacctctt cttatcgttc aaatccgatt ggtctggcgg
     4501 agtttaccct gtcacgccgc gatccaggtt atgttggcag aagtaaagcg actgctgagc
     4561 tggaaaatca gcgtctggcg gggaatgtca gcgagatggc agatgtgttt gcgcgcatta
     4621 agcagattgc tggtcaggag catattgatc cgctgcaaac tgaaattggc agcatggttt
     4681 atgcggtgaa accaggcgat ggttctgcgc gtgaacaggc ggcgagttgt cagcgtgtga
     4741 ttggcggtct ggcgaatatt gccgaggagt acgcgactaa acgctatcgt tctaacgtca
     4801 tcaactgggg gatgttaccg ctgcagatgg cggaagcgcc aacctttgaa gtgggggatt
     4861 acatttacat ccctggcatt aaagcggcac tggataatcc gggtacgacg tttaaaggtt
     4921 atgtgatcca tgaagatgcg ccggtaacgg agattacgct gtatatggaa agtctgactg
     4981 ccgaagaatg cgagattatc aaggcgggta gtttgattaa cttcaataaa aaccgtcaga
     5041 tgtaaaaagc gccatgtgga tgtaggtcgc atccggcact tattgtcgga tacgatgctg
     5101 gcgcatctta tccgacctac aaatcgcatc gaaccgtagg ccggataagg catttacgcc
     5161 gcatccggca aaaagttaat agcttttact tcttcgcctc tgcaaccact ttgctaccca
     5221 cgccgcggtt attgtattcc cacatgcggt tgtagttagt gtcattcaga ttgcgctgta
     5281 cttcgtcgct atcgtcaacg ctgccggtat tacccgcaaa cggacgatta gagatcaccg
     5341 catcggccca cggtttggct gtgttaaagc cttcgttgat gacgctatca cggatcacta
     5401 cctgaccgtt ggtattggca tcaacatcca gcgagcggcc cagttgcgcc acgccatcac
     5461 cggaagcatt gaaacggctg tttacggcga ggaaaccgta atagatgtta gacagcgtag
     5521 ccggtgcaaa cacatacgct tcttgctggg tacgggagtt caccacgcgg aattcggtgt
     5581 tatcgaacac cactgcgccg cgaccagaaa cgatatccac atccccttca atatagctgt
     5641 tggtcaccag cgtacgcggc tgacgattcg tttccagacg gttctgcaca ccgctgttgg
     5701 tgacaaagaa ggtgttctga cgaccgagaa tgttgacgtt attgatctgc actttgtcgc
     5761 catcagtacg cagtgccacc gccggatggt tacccgcatc tacgctatcg cccagcgtgt
     5821 tttcgatggt caggttttgc agttgcaggc cattgttttg tgaccagaag accgcagagc
     5881 agagaacacc gatactgtcg ctgcgtttac tctggcagct atcgtacata taccacgccg
     5941 gtttacttgg catatatttg ccgcgcgggt tgacgtcgtg acgccagtcg gcagggctca
     6001 tgccaccatc aagggaaagc ccaatcttca catcaatcgg tttttcacct gtaccgtata
     6061 gagtaattcc acccggagcg gcagggacat acaccgttcc ctgatactca ccaggcatca
     6121 cggcaatata ctggcgcttg ttggtacgct tgataattgc cgcatctacc gccgcctgaa
     6181 tcgtggtatg cgttacacct tgagtacccg ccgggccgac aacaaagtca ggttgcgcag
     6241 gcagggtaat cggggaagga ttccacgctg ccgcacctgg tgtcagggat gcaaaatagt
     6301 gttgagcatc gaaattctgc gcttcttttg ccgacagaat cgggcgagaa gaggtaccag
     6361 gcgcggtttg atcagaagga cgttgatcgg gcggtgttga gctacaggcg gtcagcgtca
     6421 cgccaaaagc caatgccagc gccagacggg aaactgaaaa tgtgttcaca ggttgctccg
     6481 ggctatgaaa tagaaaaatg aatccgttga agcctgcttt tttatactaa gttggcatta
     6541 taaaaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     6601 tcattatttg atttcaattt tgtcccactc cctgcctctg tcatcacgat actgtgatgc
     6661 catggtgtcc gacttatgcc cgagaagatg ttgagcaaac ttatcgctta tctgcttctc
     6721 atagagtctt gcagacaaac tgcgcaactc gtgaaaggta ggcggatccc cttcgaagga
     6781 aagacctgat gcttttcgtg cgcgcataaa ataccttgat actgtgccgg atgaaagcgg
     6841 ttcacgacga gtagatgcaa ttatggtttc tccgccaaga atctctttgc atttatcaag
     6901 tgtttccttc attgatatcc cgagagcatc aacatgcaat gttgtaggga tggcaatttt
     6961 tacgcctgtt ttgctttgtt cgacataaag atatccatct acgatatcag accacttcat
     7021 ttcgcataaa tcaccaactc gctgcccggt aacaacagcc agttccattg caagtctgag
     7081 ccaacatggt gatgattctg ctgcttgata aattttcagg tattcgtcag ccgtaagtct
     7141 tgatctcctt acctctgatt ttgctgcgcg agtggcagcg accgggtttg ttgttatatg
     7201 gccttcagct attgcctctc ggaatgcatc gctcagtgtt gatctgatta acttggctga
     7261 cgccgccttg ccctcgtcta tgtatccatt gagcattgcc gcaatttctt ttgtggtgat
     7321 gtcttcaagt ggagcatcag gcagccccct ccttattgct ttaattttgc tcatgtaatt
     7381 tatgagtgtc ttctgcttga ttcctctgct ggcgaggatt ttttcgtagc gatcaagcca
     7441 tgaatgtaac gtaacagaat tatcactgtt gattctcgct gtcagaggct tgtgtttgtg
     7501 tcctgaaaat aactcaatat tggcctgtat tgcttcagtg attgctatcc tcctgtctcg
     7561 gcctaaacca aactctttac ccgtccttgg gtccctgtag cagtaatatc cattgtttct
     7621 tatataaagg ttagggggta aatcccggcg ctcatgactt cgccttcttc ccatttctga
     7681 tcctcttcaa aaggctacct gttactggtc gatttaagtc aacctttacc gctgattcgt
     7741 ggaacagata ctctcttcca tccttaaccg gaggagggaa tatcctgcat tcgcgcaccc
     7801 atcgacgaac tgtttcaagg cttcttgggc gtcgctggcg tgcgttccac tcctgaagtg
     7861 tcaagtacat cgcaaagtct ccgcaattac acgcaagaaa aagccgccat caggcggctt
     7921 ggtgttcttt cagttcttca attcgaatat tggttatgtc tgcatgtgct atctgcgccc
     7981 atatcatcca gtggtcatag cagtcattga tgttctctgc ttcgataact ctgttgaatg
     8041 gttctccatt ccattcacct gtgactcgga agtgcattta tcatctccat aaaacaaaac
     8101 ccgccgtagc gagttcagat aaaagaaatc ctcgtcagtg cgaggatgct gttcattgct
     8161 gctatacact tttttgctct caacgtaagc ggtagctcat tctgttgggt tggtgcagtt
     8221 gcttttagga aatgctattt accccttaaa cgtcggctga aagagctaaa atccatgcaa
     8281 aaaatttacg caattttgtg tattattgtg cagtaagtaa tgagctattt tctgcgcaaa
     8341 aaatggatgg taaatttgtc cggggcagga aaaattttat gggcgctaaa catgaaaaaa
     8401 gattcgtatc cttatttgat ttgcatgaca gtttcaggcc tgatctttat tttccttttc
     8461 ttctggtggc gggcagatat ttacagggtc acgtttctta atcagagtat atcccactat
     8521 tacattctgt ttagcatggg aatagctttt ctgttatctc tgttttgggt taagaagggg
     8581 atagtaaaac aaagcggctg gaagagtctg tcagcatacc ttaaggttta tgcagggatg
     8641 tgcatatttg ctggattttt tctgattata ccccttacaa cactaactta ttttttgcct
     8701 ggagagacat cgtcttatgt tgcaccgtat cggtatactt ccggtagttc aaaaagttgt
     8761 tctggagctg aggtggatga ccccgatcta catgagaata ttcgcatttg ctatccgtat
     8821 ggcaattatg agtacgataa tattatctat gttgaaaaga aaattaatac attaggtgcg
     8881 gtagtaacat atgcacagac cgcgcgtgat gatactgaat gagatagtat atagcgggca
     8941 agttttagtt aatttatcga ggtaatataa tttacctcga ctcgtttatt ctggtattaa
     9001 tatttcgctt tacgatcgat ttttatctga tgatatcatg cggttttcat atactgactt
     9061 actgtctttt ctccgttagc gattttctcc tgctcagcga tgattttatc tttggcttct
     9121 agttaatttc gctcacttcg aacctctctg tttactgata agttccagat cctcctggca
     9181 acttgcacaa gtccgacaac cctgaacggc caggcgtctt cgctcatcta tgggatcgcc
     9241 acactcacaa caatgagtgg cagatatagc ctggtggttc aggcggcgca tttttattgc
     9301 tgtgttgcgc tgtaattctt caatttctga tgctgaatca atgatgtctg ccatcttcca
     9361 ttaatccctg aattgttggt taatacgctt gagggtgaat gcgaacaata aaaaaggagc
     9421 ctgtagctcc ctgatgattt tgcttttcat gctcaccgtt ccttaaagac gccgtttaac
     9481 atgccgatcg ccaggcttaa atgagtcggt gtgaatccca tcagcgttac cgtttcgcgg
     9541 tgcttcttca gtacgctacg gcaaatgtca tcgacgtttt tatccggaaa ctgctgtctg
     9601 gcttttttga tttcagaatt agcctgacgg gcaatactgc gaagggcgtt ttcttgctga
     9661 ggtgtcattg aacaagtccc atgtcggcaa gcataagcac acagaatatg aagcccgctg
     9721 ccagaaaaat gcattcagtg gttgtcatac caggtctctc tcatctgctt ctgctttcgc
     9781 caccatcatt tccagctttt gcgaaaggga tgtggctaac gtatgaaatt cttcgtctgt
     9841 ttctaccggt attggcacaa acctgactcc aatttgagca aggctatgtg ccatctcaat
     9901 actcgttctt aactcaacag gagatgcttt gtgcatatcg cctcccgttt attatttatc
     9961 tcctcagcca gcgctgggct ttcagtggat tttggataac agaaaggccg ggaaataccc
    10021 agcctcgctt tgtaacggag tagacgaaag tgatcgcacc tacccggata ttatcgtgag
    10081 gatgcgtcat cgccattgct ccccaaatac aaaaccaatt tcagccagtg cctcgtccat
    10141 tttttcgatg aactccggca ccatctcgtc aaaactcgcc atgtaatttt catcccgctc
    10201 aaccacgaca taatgcaggc cttcacgctt cattcgtggg tcatagttgg caaagtacca
    10261 ggcatctttt cgcgtcaccc acatgctgta ctgcacctgg gccatgtaag ccgactttat
    10321 ggcctcgaaa ccaccgagcc ggaatttcat gaaatcccgg gaggtaaacg ggcatttcag
    10381 ttcaaggccg ttgccgtcac tgcataaacc atcgggagag caggcggtgc gcatactttc
    10441 gtcgcgatag atgatcgggg attcagtaac attcacgccg gaagtgaact caaagagggc
    10501 tctggcgtcg ttctcgtact gtattcccca ggccagcgcc ttagcgttaa cttccggagc
    10561 cacaccggtg caaacctcgg caagcagggt gtggaagtag gacattttca tgtcaggcca
    10621 tttctttccg gagcggggtt ttgctatcac attgtgaact tctgaagcgg tgatgacgcc
    10681 gagccgtaat ttgtgccacg catcatcccc ctgttcgaca gctctcacgt cgattccggt
    10741 acgctgcagg ataatgtccg gtgtcataca gccaccttct gttcagaggc tttctgtttc
    10801 aggaatccaa gagctttcat tgcttctgcc tgtgtcagtt ctgacgatgc gcgaatgtcg
    10861 cggcgaaata tctgggaaca gagcggtaat aagtcgtcat cccatgtttt gtccagggca
    10921 atcagcagag tgttaatctc ctgcatggtt tcatcgttaa ccggagtgat gtcgcgttcc
    10981 ggctggcgtt ctgcagtgta tgcggtattt tcgacaatgc gctcggcttc atccttgtca
    11041 tagataccag caaatccgaa ggccaggcgg gcacactgaa tcatggcttt atgccgtaac
    11101 atccgtttgg gatgcgactg ccacggcccc gtgatttctc tgccttcgcg ggttttgaat
    11161 ggttcgcggc gg
//
LOCUS       DAZFJL010000149        11017 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFJL010000149 DAZFJL010000000
VERSION     DAZFJL010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.151"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..867
                     /locus_tag="ACHJ8C_003969"
     CDS             <1..867
                     /locus_tag="ACHJ8C_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB C-terminal
                     domain-containing protein"
                     /protein_id="HFO5553749.1"
                     /translation="YHLVRPEPEGHVVLFPEGHAEFYVPSAEFTSVSVALRLGAMIAQ
                     MDKRVDVAEQAALERTIDHNDALSPTEKCSLHAYLIWRLNTPANQAGLKGKIEQLSDK
                     DKSTIGNVIISVACADGKIDPAEIKQLEKIYTSLGLDSSTVTGDIHRQSTAETAPTAT
                     LQTPSATSSTFSLDERILARHESDTTDVRQLLNTIFTEDEPADESPAEIPSHTGVGLD
                     EAHHLLYQRLLEKERWARNEVAELCQQFNLMLSGAIEAINGWSFEQVDAPVLDDDDDI
                     YVDLEIAQELKG"
     gene            871..2162
                     /locus_tag="ACHJ8C_003970"
                     /pseudo
     CDS             871..2162
                     /locus_tag="ACHJ8C_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            2159..4354
                     /locus_tag="ACHJ8C_003971"
     CDS             2159..4354
                     /locus_tag="ACHJ8C_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO5553750.1"
                     /translation="MMNEYQRLDPRIQKWIYQQGWTDLRELQKKAVPSILTGDRDVLI
                     SAATAAGKTEAFFLPACSAIADIQGGFGILYISPLKALINDQYRRLESLGNALEMPVT
                     PWHGDVAQSKKLKAKKNPAGILLITPESLEAMLIRNAGWLKQAFAPLAYIAIDEFHAF
                     IGSERGMQLLSLLNRIDHLLGRIDNPVPRVALSATLGELERVPLSLRPNQRLPCDIIT
                     DSQTHATLKVQVKGYLEPLTTSSQQPPPSAETQICHDIFRLCRGDSHLVFANSRKRTE
                     SIAATLGDLSEINIVPNEFFPHHGSLSRDLRETLEQRLQQGNLPTTAICTMTLELGID
                     IGKVSSVVQVTAPHSVASLRQRMGRSGRRDSPAILRMLIAEHELTPTSGIVDQLRLQL
                     VQSLAMIRLLIGSKWFEPADTRQMHYSTLFHQILAIVAQWGGVRADQIWSQLCQQGPF
                     QKVRIDDFKTLLKHMGENQFLTQLSSGELVLGVEGERQVNQYTFYAVFSTPEEFRIVA
                     GNKTLGSIPVDSPLMPDQHIIFGGRRWKVTDIDSDKKVIYVEATKGGQPPLFGGQGMS
                     IHDVVRQEMLTIYREGDYRITVGDRKADFADTTARNLFDEGLHCFRNNNLASECFIQQ
                     GQHVYILPWLGDQTVNTLSALLIQRGFKAGSFAGVVEVEKTTVAEVKQTLACALQKGL
                     PSESRLAESIIEKCLEKYDEYLPEALLTQEYGLRAFNVQRVEEWLQGHL"
     gene            complement(4405..5283)
                     /locus_tag="ACHJ8C_003972"
     CDS             complement(4405..5283)
                     /locus_tag="ACHJ8C_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wadjet anti-phage system protein JetD
                     domain-containing protein"
                     /protein_id="HFO5553751.1"
                     /translation="MTEAEPLSYNALVWLKKLDHRLKAKYSEKKNTGGKIVTQVLRWC
                     IALDLIPANSLLLPEFIFTVALLNKISDIQQRLKQANFRDDLSMKNRIESAQLSDQEI
                     KTAGIRPQQHRVLLRLNQPLVNALGMTVEVVDTDWRNITLTQFDVLIVVENQDCFYHL
                     ALFDYSQCDFHSPLIIYRGDRTYSKGSVALKRCWLKTGKTAIYFGDFDPKGVSIAMNE
                     GYHSMLLPSPAVVIKKSSSVMFPDAQLKFLPELLRGEVHCHFRDYLLVLDKHQALRQQ
                     KMQGEILKMVMLKASD"
     gene            complement(5280..6518)
                     /locus_tag="ACHJ8C_003973"
     CDS             complement(5280..6518)
                     /locus_tag="ACHJ8C_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553752.1"
                     /translation="MAQENSPLSDLFKNGAERLTLLGKHHHALMKGYVDGFIDETVFS
                     EQALKKLIAARIVYRPDEQQPLRLRPLVSELIASMVADESRRQINADVAEKLEQTRNK
                     VQAWRDAYYKGDYSIAERHMQRLTELIHDLSGQFEEAIDSLWHRLNNNFGFVSSLNEK
                     INENARAQNQVRRLLDGLDLIVFQELIEFAENNSSLRKLLVSQLQLRVSLHHSSLLAV
                     QQRLVDLMAKFRQQQSRTLLVTNMAAFLRQHPQYQPASYPERREVPDVFNIAASLSLQ
                     ASISLDRPVDRQVMAEMLFALSRASVMPLPVPKSAGSVILPVDEEVAARQVALKMDVE
                     DLYVFAAGQSGPVSALHYLTENHLNWDSEIWLYQVITEYQSLPLADKPRFWQRCDERQ
                     ASPVNDLRIISDVSIGVRVK"
     gene            complement(6518..7141)
                     /locus_tag="ACHJ8C_003974"
     CDS             complement(6518..7141)
                     /locus_tag="ACHJ8C_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553753.1"
                     /translation="MLEYGPLIKRLLTGEFICRINDPDAYRHLQDESTRQAMDNYLRP
                     LNYRIVSNETQTVYYAGFCEMNRDTRSQLMNQFKDIISSLLPLLEWLQLIQETQGRES
                     TLTAGDYIRLSEIMARVEDNQSLQQRLNQLSSDRFFNCKSDSIDLQLKQIFRRLKEHG
                     YVRQPNAEQQIFIATGKIDYLIDVIRFIRDEEHLPVEDTPPVQQELL"
     gene            complement(7141..10806)
                     /locus_tag="ACHJ8C_003975"
     CDS             complement(7141..10806)
                     /locus_tag="ACHJ8C_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5553754.1"
                     /translation="MPQLLRIILIHTHLPGAVEIQLDQHTNICGTNASGKTTLQRLVP
                     VFYGEQPNKVVPRTRKKFDEFYLPCSNSYIIYEYQRETGDNCLVVLTGKSEGGVEYRF
                     VSAPYHPEFFLRYTEDGAKGLSYNEWSAQMRSRDDVSVSAKIGSTTEYRSIIQNDVAS
                     LRGTSTEAMRLRRLAASFSLVSGNYKLRHIEKLVSAVHAGEGKMETLKTMLAAIFADD
                     GVTLPQTKVKSSFAREWIQKMRQSLRYDKLQQEFELLQRLGLKLDDTEAQLTALHPLL
                     RNDEQIQKQKRADAEQQFIQRRGELIQLEDGFNQQSRELNDQLSKTQSELKTVQSWLD
                     YVREENDRYERKDIRLLQTHMDELPQWREALLAQSEQYQLMQDQVGELSRKFEQHKAK
                     LQESFYYQNQLIQQKIQDFQWEKEVVRDKFDSQKEIFRQRFEELKKQQENHYGEQLAI
                     ISRDRTTIGIELKHSQLTTEENEERQIAELRIEQAQECSLQLGDRRQNAQTKLSEARK
                     QQDSAEKQLHDARADLHQAEEQLQQLHRQLDPEQGTLRHFLRSRYEDWEQNLGKVLNK
                     NLLERKDLQPDLRELSESLYGLQLELSVLDMPDFAQDEASIRHNLDKAEQIRAHAEEA
                     KKTAEASFQEHHQNVKQLQSQADGCERQYQRQNQEVGYARDARTRLIAEHGALEQERR
                     QEKRARLSALEKAHTELMQQKEQDLRSLTDEHNAQTLQWSVDLQSEQQQIDEKVEELS
                     RQIDKKRADMEEQIRIFQQALDDNLAKKGIDPREVKILRERIETLKKDIQAVEERRDE
                     LTEWQRFIKLDWEQIRPEKQKQEGELSQQKRELLQSERQLKAEYQNKKDKLNDEGKQY
                     DTLMRQAGELVSQLKVLLDRLLPLPLGEIVPSYIDSAADINERMSRTSDMLEQHASVT
                     RTLESKLNEFKSRFNQDTDPTFLELLDSEIGKLPDPSRTRQQLPILEKLLQLLKDKQQ
                     QLLEMGENIGGDLKKFFDVFSDINRRISQQSRRLSDAVTDDLQLEGINKSEVRILSTI
                     DQLRFWSPLKRFAKSYSEWRSSGQQLPPESYLNALMDVVELLHADERFTMESLLNLEL
                     HLNEGGEDIVIKNDRQLLEASSHGMAYLILCKYLLAFTRLLRGKSDAVIHWPIDEIGT
                     LAYHNVEKLFIACSHNNIVIVGAFPNAESDVLILFKHRYLLDADQLEPTQRRLKRIQP
                     KVSRLAARLAQMEQEAM"
BASE COUNT         2821 a         2776 c         2506 g         2914 t
ORIGIN      
        1 tatcaccttg tccggccaga accagaagga cacgttgttt tattccccga agggcatgca
       61 gaattctacg taccgtcggc ggaattcacg tcagtttccg tggcgcttcg gctgggtgcc
      121 atgattgcac aaatggacaa gcgcgtggac gttgcagaac aggccgcact ggaaagaacc
      181 attgatcaca acgatgcgct gtcgccaaca gaaaaatgct cgctgcacgc ttacctcatc
      241 tggcgactca atacacctgc aaatcaggcc ggtctgaagg gtaaaattga gcaactcagc
      301 gataaagata aatccactat tggcaacgtg attatcagcg tcgcctgcgc agatggaaaa
      361 atcgatccgg ctgaaattaa gcaactggaa aaaatctaca ccagccttgg tctggatagc
      421 agtaccgtca ccggcgatat ccaccgtcag tcaaccgcag aaacagcgcc gacagccaca
      481 ttacaaaccc catcagcgac gagcagcacg ttttctcttg atgaacggat ccttgcccgt
      541 catgaatccg ataccacgga cgtacgccag ttgctgaaca ccatcttcac cgaagatgaa
      601 cccgctgacg aatccccggc ggagatcccg tcacatactg gagtgggtct tgatgaagca
      661 catcacctgc tttaccaacg tttgctggaa aaagaacgct gggcgagaaa cgaagtcgct
      721 gagctatgcc agcagtttaa tttgatgcta agcggcgcga ttgaagcaat taatggctgg
      781 tctttcgaac aggttgacgc accagtgctg gatgatgacg atgatattta cgttgacctg
      841 gaaattgcac aagaactcaa aggataattt atgtctggca ttcgtattcg tctcaaagaa
      901 agagacgcca ttattcagtc actgaagtca ggcgttacgc ctaaaattgg cattcagcat
      961 attcaggttg gccgggtcaa tgaaataaaa gcgctgtatc aggatattga acgtattgcc
     1021 gatggtgggg cgggattcag gttgattatc ggggaatacg gctcaggtaa gacattcttt
     1081 ttaagcgttg tacgctcaat tgcgttagag aaaaagctgg tgacaatcag tgccgattta
     1141 tctccggaca ggcgcgcaac ctctattctg agctaatgaa aaatctgtct acgcgaaata
     1201 agccggatgg aaacgcatta ttaagcgtgg ttgaacgttt tataacggaa gccagaaaag
     1261 aagcagaatg tacaaatgtg tcggttccga cgattattca ccaaaaactc gccgccctgt
     1321 ctgatatggt tggcggttac gatttcgcca aagtcattga atgctactgg cagggccacg
     1381 agcaggataa tgagacattg aaatcaaatg ccatccgctg gctaagaggt gaatacacca
     1441 cgaaaaccga cgcgcgtaac gatctgggtg tgcgcaccat tatttctgat gcctctttct
     1501 acgactcgtt aaagctgatg agcctgtttg tccgtcaggc cggatacgcc gggctacagg
     1561 tcaatctgga tgaaatggtc aatctgtaca aactgagcaa tacgcaagcg cgagtggcca
     1621 actatgaaca aatactgcgt attctgaatg actgcctgca aggaacggct gagtatatcg
     1681 gctttttact cggcggcacg ccagagttcc tgttcgatcc gcgcaagggg ttgtacagct
     1741 acgaagcgct ccagtcccgg ctggcggaaa acagctttgc tcagcgtgcc ggggtgattg
     1801 attattcgtc cccttccctg cacttagcca gcttaacgcc ggaagagctc tatattctgt
     1861 tgaaaaacct tcgtcacgtt tattccggcg gcgatgcgga taactatctg gttcctgatg
     1921 atgcgctgac ggcattctta agccactgta gcaatactat cggtgatgcc tatttccgta
     1981 cgccacgaaa cacgattaaa gccttcctgg atatgctggc cgtgctggaa caaaatccgt
     2041 ccattcagtg gtcacagcta attgccggtg tcgcgatcgc agaagaaaaa cccagcgata
     2101 tggatgaaat aacatcggca gaagatgccg atgaggacgg tctggctgac ttcagattat
     2161 gatgaacgaa taccagcggc tggatccacg catacagaag tggatatacc agcagggatg
     2221 gacagatctc cgggaactgc aaaaaaaagc cgttccatcg atattaacag gggatcggga
     2281 tgttctgatc agcgccgcga ctgccgcagg taaaacggaa gcgtttttcc tgcccgcctg
     2341 ttctgccatt gcggatattc agggcggctt tggcatttta tacatcagcc cgcttaaggc
     2401 cctgattaac gatcagtatc gacggctgga gagcctcggc aatgcgctgg agatgccggt
     2461 cacgccctgg catggtgatg ttgcgcagag caaaaagctg aaagcaaaga agaatcctgc
     2521 cggtattttg cttattaccc cggagtcgct ggaagcaatg ctgatccgca atgcgggatg
     2581 gttaaagcag gctttcgctc cactggcata tatcgccatt gatgaattcc acgccttcat
     2641 cggttctgag cggggtatgc agcttctctc tctgttaaat cggatcgatc acctgctggg
     2701 aagaatcgac aatccagttc ctcgggtcgc actcagcgca acgctgggag aactggagcg
     2761 agtaccgtta tctctgcggc caaatcaacg tctgccctgc gacattatta ctgacagcca
     2821 gactcacgct acgctaaaag tgcaggtgaa aggttatctg gaaccgctga ccacctcgag
     2881 ccagcaaccg ccgccgtcgg cagagacgca aatctgccat gatatatttc gcctctgtcg
     2941 cggtgattcc catctagtgt tcgctaatag tcgcaaacgg actgaaagca ttgccgctac
     3001 gcttggcgat cttagtgaaa taaacattgt gcccaacgag ttcttccccc atcacggatc
     3061 gctgtccaga gatctgcgtg aaacgctgga gcagcggctt cagcaaggta acttaccgac
     3121 caccgccatc tgtacgatga cgctagaact tggcatcgac atcggcaaag tcagctccgt
     3181 cgtgcaggtt actgcgcctc attccgttgc cagcctgcgc cagcgtatgg ggcgttccgg
     3241 tcggcgcgac tcgcctgcca tattgagaat gctgattgcc gaacatgaac tgacgccaac
     3301 ttcaggcatt gttgatcaac tcaggctcca gctcgttcag tcgctggcca tgatccgctt
     3361 acttatcggc agcaaatggt ttgagccagc cgatacccgg cagatgcact attccaccct
     3421 gttccatcag atcctggcga tcgtagcgca gtggggaggg gtgcgtgccg atcagatctg
     3481 gtcacagcta tgccaacaag ggccatttca gaaagtcaga atcgatgact tcaaaacgtt
     3541 attgaagcat atgggggaga accagtttct gacccagcta tcaagcggtg aactggttct
     3601 gggcgtcgaa ggcgaacgtc aggtgaatca atataccttc tatgccgtgt tcagcacgcc
     3661 ggaagagttt cgtattgtgg cggggaacaa aacgctgggc tctatccccg tagattcccc
     3721 actaatgcct gaccaacaca ttattttcgg cggtcgacgc tggaaggtaa ccgatatcga
     3781 tagtgataaa aaagtcatct atgtcgaggc gacaaagggt gggcagccgc cgttatttgg
     3841 cggacaaggg atgtccattc atgatgtcgt ccgccaggaa atgctcacca tttatcggga
     3901 aggcgactac cgtatcaccg ttggcgatcg caaagccgat tttgccgata ccacggccag
     3961 aaacctgttt gatgaagggc tgcactgttt tcgcaacaat aatctggctt cggaatgttt
     4021 tattcagcag ggacaacacg tctacattct tccctggctg ggcgatcaaa ccgtaaatac
     4081 actgtcggca ttgcttatcc agcgaggttt caaggcaggc tcatttgctg gtgtggttga
     4141 agtggaaaaa accacggtag cggaagttaa gcagacgtta gcctgcgcac ttcagaaagg
     4201 gctaccttca gaatcccgtc ttgccgaaag catcattgaa aagtgcctcg aaaaatatga
     4261 tgagtattta ccggaggcgt tgctgacgca ggagtatggg ttacgtgctt ttaatgttca
     4321 gcgcgtggag gagtggttgc aggggcattt gtaggataat gacctgctca tcagataaac
     4381 atacacggcc agcaacaaaa tattttaatc tgatgctttc aacataacca ttttcaagat
     4441 ttcaccctgc attttttgct gcctcagcgc ctgatgtttg tccaaaacca acagatagtc
     4501 acgaaaatgg caatgaacct cacctctcaa taattcaggt aagaacttta actgcgcatc
     4561 aggaaacatc actgaagagg atttttttat cactacggct ggggaaggta ataacataga
     4621 atgataacct tcattcatcg caatactgac ccctttcggg tcaaaatcac caaaatagat
     4681 cgctgttttc ccggttttca accagcaacg ctttaatgca acagacccct tgctatatgt
     4741 ccgatcgcca cggtaaataa tcaggggaga gtggaaatca cattgggaat aatcaaaaag
     4801 cgccagatgg tagaaacaat cctgattctc cacaacgata agaacatcaa actgcgtcag
     4861 cgtaatatta cgccaatccg tatcaacaac ctcaacagtc atccctaacg cattgactaa
     4921 aggctgatta agacggagca gtacccggtg ctgctgtggt cgaattcccg ccgttttaat
     4981 ctcctgatcg cttaactgtg cgctctcaat acgatttttc atactaagat cgtcacgaaa
     5041 gtttgcctgt ttaagacgtt gctggatatc tgaaatctta ttaagcaacg ccactgtaaa
     5101 aatgaattca ggcagcaaca aactgttcgc cgggatcaaa tccagcgcaa tacaccaacg
     5161 caaaacctga gtcacgattt taccgccagt atttttcttc tcgctatatt tcgctttcag
     5221 gcgatgatca agttttttca gccagacgag tgcgttatac gataacggtt ctgcttcggt
     5281 cattttaccc tcacgccaat actcacatcc gatataatac gcagatcgtt taccgggctg
     5341 gcctgacgct catcacaacg ctgccagaaa cgcggtttat ccgctaacgg caatgattga
     5401 tattccgtaa taacttggta aagccatatc tcactgtccc agttcagatg attttccgtc
     5461 agataatgca gtgcgctcac cggcccggat tgtccggcag cgaaaacata caaatcctca
     5521 acatccattt taagtgcaac ctgacgagca gcgacctctt catcaacggg aagtatcact
     5581 gaacctgcac tttttggcac aggtagaggc ataaccgatg cccgagacag agcaaacaac
     5641 atctccgcca tgacttgtcg atccacaggt ctgtcgagcg aaatggaggc ttgcagtgaa
     5701 agtgaagccg caatattgaa tacatccggc acttcccggc gttccgggta gctggcgggt
     5761 tgatactgtg gatgctgacg caaaaaagcc gccatattcg tcactaataa tgtgcgcgac
     5821 tgctgttgac gaaatttagc catcagatcc accagacgct gctgaacggc cagtaatgaa
     5881 ctatggtgaa gactgacacg caactgtaac tggctcacta ataatttacg tagtgaactg
     5941 ttattttccg caaactctat cagttcctgg aaaacgatca gatccagtcc atcaagcaac
     6001 cggcgaacct ggttttgcgc ccgtgcattc tcattaattt tttcattcag actgctgacg
     6061 aaaccaaagt tattattcag acgatgccag aggctgtcga tagcctcttc aaactgcccg
     6121 ctgagatcgt gaataagttc agtcagcctc tgcatgtgtc tttcagcaat actgtaatca
     6181 cctttataat acgcatcgcg ccaggcctgt accttattgc gagtttgctc cagcttttca
     6241 gccacatccg cgttaatctg ccgtctactc tcatcagcaa ccatgctggc gatgagttcg
     6301 ctgaccagcg ggcgtagcct cagcggctgt tgttcatcag ggcgatacac aatacgtgcg
     6361 gcaatcagct tttttaatgc ctgctcgcta aaaaccgttt catcaataaa cccatcgaca
     6421 taacccttca ttaaggcgtg atggtgtttt cccaataatg tcagcctttc agcgccgttt
     6481 ttaaacaggt cagatagcgg agaattttcc tgcgccatta cagaagctcc tgctgaacag
     6541 gcggcgtgtc ttcaaccggt aaatgctctt catcacggat aaagcggatc acgtcaatca
     6601 agtaatcaat tttccccgtg gcaataaata tctgctgctc ggcgttgggt tgtctgacat
     6661 agccgtgctc tttcaaacgc cggaaaattt gtttgagctg aagatcaatg gaatcactct
     6721 tacaattgaa aaaacgatcg ctggaaagct gattcagacg ctgttgcaaa ctctgattat
     6781 cctctacacg cgccatgatt tccgacaacc tgatatagtc gcccgctgtc agcgtactct
     6841 cccgtccttg cgtctcctgt atgagttgta gccactcaag cagcggcaac agagaagaga
     6901 tgatatcctt aaactgattc ataagctggc tgcgggtatc gcgattcatc tcacaaaagc
     6961 ctgcataata gaccgtttgt gtttcattac tgacaattct gtagttaaga gggcgcaaat
     7021 aattatccat cgcttgccgg gtactctcat cctgtaaatg acgataagca tccgggtcgt
     7081 tgatacggca aataaattcc cctgtcagta gccttttgat taaaggacca tattccagca
     7141 ttacattgcc tcctgttcca tctgcgccag tcgggcagcc aggcggctca ctttcggctg
     7201 gatccttttc agcctgcgct gagttggctc aagctgatcg gcatcaagca aataacgatg
     7261 cttaaacagt attaacacat ccgattccgc attcggaaaa gcgcctacga tcacaatatt
     7321 attatggcta caggcaataa ataatttctc cacattgtgg taagccagcg taccgatttc
     7381 atcaataggc cagtgaataa cggcgtctga tttgcctctt aacaaacgcg tgaacgccaa
     7441 aagatacttg cacaagatca gataggccat accatggctt gacgcttcca gtagctggcg
     7501 atcgtttttg ataacaatat cttccccacc ttcattaagg tgaagttcga gatttaataa
     7561 cgattccata gtgaaacgtt catcggcatg aagcagttca accacatcca tgagggcatt
     7621 caaatagctt tcgggaggta gctgctgacc agacgagcgc cactcactgt agcttttagc
     7681 aaagcgtttt aatgggctcc agaatcgtaa ctggtcgatg gtcgataaaa tgcgaacctc
     7741 tgatttattg atgccttcca gttgcaaatc gtcggtaacc gcatcactta agcgtcgact
     7801 ttgttgcgaa atccggcgat tgatatcact gaaaacatca aagaattttt tcagatcgcc
     7861 accgatattt tcccccatct ccagcaattg ctgctgtttg tctttaagaa gctgtagtag
     7921 tttctcaagt attggcaact gctggcgggt ccttgaagga tcgggtaatt taccaatctc
     7981 agagtccagt aattcaagga aagtagggtc agtatcctga ttaaagcgtg acttaaactc
     8041 attgagtttg ctctccagcg tacgggtcac gctggcgtgt tgctccagca tatcgctggt
     8101 acgcgacatc cgttcattaa tatcagcggc ggaatcaata taagacggaa caatttcgcc
     8161 cagcggcaga ggcaataatc ggtccagcaa tacctttaac tgactaacaa gctcaccggc
     8221 ctgacgcatc aacgtatcat attgttttcc ctcatcatta agcttgtctt ttttattctg
     8281 atactctgct tttaactgac gttctgactg caatagctct cgcttctgct gagagagttc
     8341 accttcctgc ttctgtttct caggccgtat ctgctcccag tcaagtttga taaaacgctg
     8401 ccattcggtc agttcatccc gacgttcttc aaccgcctga atatcctttt taagcgtttc
     8461 aatgcgctcc cgcagtattt tgacttcacg aggatcaatt cccttcttcg ctaaattatc
     8521 gtccagagcc tgctggaata ttcttatctg ttcctccata tcggcacgtt ttttgtctat
     8581 ctgccgggac agttcttcca ccttctcatc aatttgctgc tgttcactct gaaggtcgac
     8641 actccattgc agcgtttgcg cattatgttc gtccgttaat gagcgaagat cttgttcttt
     8701 ttgttgcatt aattcggtat gcgctttttc taatgcgctt aagcgggcac gtttttcctg
     8761 ccgacgttct tgctccagcg ccccatgctc tgcgattaaa cgagtacggg catcccgggc
     8821 atagccgacc tcttgatttt gacgttgata ttgtctttca catccatccg cttgcgattg
     8881 cagctgcttg acgttctggt ggtgctcctg aaagctggct tctgccgttt ttttcgcttc
     8941 ttcagcatgc gccctgattt gctctgcctt atcaagattg tgtcgaattg aagcttcatc
     9001 ctgcgcaaaa tcgggcatat ccagtacgct taattccagt tgcaagccgt acagtgattc
     9061 cgacagttcc cttagatcag gctgcaagtc cttacgttcc agcaggtttt tattcaaaac
     9121 tttgcccaaa ttctgctccc agtcctcgta acgtgaacgc aaaaagtggc gtaatgtccc
     9181 ctgttcagga tccaactgac gatgcagttg ctgcaattgc tcctcagcct gatgcaggtc
     9241 ggcgcgggca tcatgcagtt gcttttctgc gctatcttgc tgtttacggg cctcggacag
     9301 ctttgtctgc gcattttgtc gccgatcgcc cagttgcaat gaacactcct gagcttgctc
     9361 aatacgcaat tcggcaatct gacgttcctc attttcttca gtggttaact gagaatgttt
     9421 aagctcaatg ccaatagtcg tccgatcgcg agaaattatc gccaactgct cgccataatg
     9481 attttcttgc tgttttttta gttcttcaaa gcgctgccga aatatctctt tctggctatc
     9541 aaacttatcc ctgacgacct ctttttccca ctgaaaatcc tgaatttttt gctggatcaa
     9601 ctggttctgg taataaaaag attcctgaag ttttgctttg tgctgttcaa attttctgga
     9661 aagctccccc acttgatcct gcatcagctg atattgctca ctttgcgcta aaagcgcctc
     9721 ccgccactgg ggtaattcgt ccatgtgcgt ctgcaaaagg cggatatctt tacgttcata
     9781 acgatcgttt tcttcccgga cataatccag ccatgactga accgttttta attcgctttg
     9841 cgttttactt aattgatcgt tcaattcacg actttgctga ttaaagccat cctctaattg
     9901 gataagctct ccccgccgct gaataaattg ctgttctgca tccgcccttt tctgtttctg
     9961 aatttgttca tcatttctta acagggggtg gagcgcggtt aactgcgcct ccgtatcatc
    10021 cagtttcagc cccaggcgct gtaagagttc aaactcctgc tgcaatttat cgtaacgcag
    10081 ggactggcgc attttttgga tccattcccg cgcaaagcta ctttttactt ttgtttgagg
    10141 aagcgtaacg ccatcatcgg caaaaatcgc cgccagcatg gttttgagcg tttccatttt
    10201 gccttcaccg gcatgaaccg cgctcactaa tttctcaata tgcctgagct tgtagttacc
    10261 actcaccaga ctaaacgatg ccgccaggcg gcgtagtctc attgcctccg tcgaagtacc
    10321 gcgtaatgac gcgacatcat tctgaatgat gctgcggtac tcggttgttg aacctatttt
    10381 ggctgaaact gagacgtcgt cccggctgcg catctgggcg gaccattcgt tataactcag
    10441 ccctttcgcg ccatcttccg tatagcgaag gaaaaattct ggatgataag gtgcgctaac
    10501 aaaacgatat tctacgccac cctcactttt acccgtcaaa acaaccagac aattgtcgcc
    10561 cgtttctcgc tggtattcat aaatgatata gctattgcta caggggagat aaaactcgtc
    10621 gaacttcttc cgcgttcttg gtacgacttt attaggttgt tcgccgtaga aaacaggaac
    10681 aaggcgctga agcgtcgttt ttcctgaagc attagttcca caaatattgg tatgctgatc
    10741 cagttgaatt tcaacagcgc ctggcaggtg agtgtgtata agaatgatgc gaagcagttg
    10801 tggcatgata cctccctgaa cataaaataa cctttggata atacacccca aacccttact
    10861 aaacattggg ttatttcaca attagatcag aaggcaggcg atgcaaaagt ctttatgatc
    10921 ttaaaaaggg aaccgtacca aaggttcaaa ccgaactatt tttttgacta ttaatccacc
    10981 attatgattt aaaagcaaaa atacgggtca ctctccg
//
LOCUS       DAZFJL010000150        10961 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFJL010000150 DAZFJL010000000
VERSION     DAZFJL010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.152"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            119..880
                     /gene="adiY"
                     /locus_tag="ACHJ8C_003976"
     CDS             119..880
                     /gene="adiY"
                     /locus_tag="ACHJ8C_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO5553755.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            1017..2354
                     /gene="adiC"
                     /locus_tag="ACHJ8C_003977"
     CDS             1017..2354
                     /gene="adiC"
                     /locus_tag="ACHJ8C_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO5553756.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2458..4101
                     /gene="eptA"
                     /locus_tag="ACHJ8C_003978"
     CDS             2458..4101
                     /gene="eptA"
                     /locus_tag="ACHJ8C_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO5553757.1"
                     /translation="MLKRLLKRPSLNLFSWLLLATFYISVCLNIAFFKQVLQVLPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLGSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMITNIIDTTPAESYALMTPRMLLTLGLSGVLAAIIACWIKIKPTTSRLRSVLFRGAN
                     ILISVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRSANLPLIR
                     IGEDAHRNPLMQNGKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGVCD
                     RVPHQNLTALNLPGQCINGECYDEVLFHGLEEYINNLQSDGLIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4098..4766
                     /gene="basR"
                     /locus_tag="ACHJ8C_003979"
     CDS             4098..4766
                     /gene="basR"
                     /locus_tag="ACHJ8C_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO5553758.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVSTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELNVGNLTLNMGRRQVWMSGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4767..5867
                     /gene="pmrB"
                     /locus_tag="ACHJ8C_003980"
     CDS             4767..5867
                     /gene="pmrB"
                     /locus_tag="ACHJ8C_003980"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO5553759.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIDAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWIRLKKDQYVANQI"
     gene            complement(5843..5932)
                     /gene="pmrR"
                     /locus_tag="ACHJ8C_003981"
     CDS             complement(5843..5932)
                     /gene="pmrR"
                     /locus_tag="ACHJ8C_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO5553760.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(6044..7546)
                     /gene="proP"
                     /locus_tag="ACHJ8C_003982"
     CDS             complement(6044..7546)
                     /gene="proP"
                     /locus_tag="ACHJ8C_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO5553761.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7810..8688)
                     /locus_tag="ACHJ8C_003983"
     CDS             complement(7810..8688)
                     /locus_tag="ACHJ8C_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO5553762.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIVALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVSTGGDHADFLSWLGKWSTADKQPVCWSVTQRWRTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            complement(8685..10913)
                     /gene="crfC"
                     /locus_tag="ACHJ8C_003984"
     CDS             complement(8685..10913)
                     /gene="crfC"
                     /locus_tag="ACHJ8C_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO5553763.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKIPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISQLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDHLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGIAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSTNHFLRQQQDAVNAQLAALFQD
                     DSESLSEMRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQAATDEQSTRLGVVREIFSRWLNQPD
                     WGRGNEKFSTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2768 a         2880 c         2660 g         2653 t
ORIGIN      
        1 tagtaaaact cgttattttt atgcatgttt atattcatca tacaattata taaccatttc
       61 ccggtatcgc ttcgctttag cgagaactgg tcttttattc gcactcagga gtacatgtat
      121 gaggatttgc agcgaccaac cttgtattgt tttattgact gaaaaagatg tctggataag
      181 ggtgaatggg aaagaaccta ttagccttaa agctaaccac atggcgttat taaattgtga
      241 aaataatatt atcgacgtct cctctcttaa taacactttg gttgctcata ttagtcacga
      301 catcatcaaa gattacctcc ggtttctgaa taaagatctc tcgcaaatac cagtatggca
      361 acgtagcgct acgcccatcc tcaccctgcc atgcctgacg ccagacgtct ttcgcgttgc
      421 cgcgcagcat agcatgatgc ccgctgaaac tgagtcagaa aaggaacgaa cacgtgcatt
      481 attattcact gtgctatccc gttttctcga cagtaaaaaa ttcctttcac taatgatgta
      541 tatgttacgt aattgtgtaa gtgacagcgt ttatcaaatt attgaaagcg atatccacaa
      601 agactggaat cttagtatgg tagccagttg tttatgtctt agcccaagtc tgttaaagaa
      661 aaagctgaaa agcgaaaaca ccagttatag ccaaataatc accacctgcc gcatgcgtta
      721 tgccgtaaat gaattaatga tggacggtaa aaatatctct caggtatcac agtcctgcgg
      781 ctacaacagt acgtcgtact ttatttctgt ctttaaagac ttctacggta tgacgccgct
      841 gcattatgtt agtcagcaca gagaacgcac tgtcgcctga tttttaacct taacgaagag
      901 ctatattaat aacggcatca gcgataaccc ggccgataat aattcaacta tcgaatgcag
      961 gcgtatgata tgacgtaatt tattgtcacg aagctcgcct tcgcaggagt ttaattatgt
     1021 cttcggatgc tgatgctcac aaagtgggct taatccccgt caccctgatg gtgtcgggga
     1081 atattatggg gtcaggtgtt tttctgttac ctgcaaacct ggcctccact ggcgggattg
     1141 ccatttatgg atggttggtg acgattatcg gtgcgctggg gctctcgatg gtatacgcca
     1201 aaatgtcgtt cctcgaccca agtcctggtg gttcttacgc ttacgcccgc cgctgctttg
     1261 gcccgtttct cggttatcaa accaacgtcc tctactggct ggcctgctgg atcggcaata
     1321 tcgccatggt ggtcattggc gtaggatatt taagttactt cttcccgatt ctgaaagacc
     1381 cattggtatt aaccatcacc tgcgtcgtgg tgctgtggat cttcgtcctg ctgaacattg
     1441 tcggtccgaa aatgatcacc cgcgtacagg ccgttgctac cgtgctggcg ctgatcccca
     1501 tcgtcgggat tgccgtattt ggctggttct ggttccgtgg tgaaacctat atggcggcat
     1561 ggaacgtcag cggcctgggc accttcggtg cgattcaaag tacccttaac gttacgctgt
     1621 ggtcgttcat cggtgtggaa agtgcctccg ttgccgcagg tgtggtgaaa aacccgaaac
     1681 gcaacgtccc tatcgccacc attggcgggg tattgattgc tgccgtttgc tatgtacttt
     1741 ctaccaccgc gattatgggg atgatcccta atgccgcact gcgcgtttct gcttcgccat
     1801 tcggtgatgc cgcacggatg gcgttgggtg acaccgccgg ggccattgtt tccttctgcg
     1861 cagctgcggg ttgcttaggt tcactgggcg gctggacgtt gctggcgggt caaacggcga
     1921 aagccgctgc cgatgacgga ctgttcccac cgatttttgc ccgtgtaaat aaagcgggca
     1981 cgccagtggc ggggctgatt atcgtcggta ttttgatgac catcttccag ctcagtagca
     2041 tttcgccaaa cgcgactaaa gaattcggtc tggtttcttc cgtgtcggtc atctttacac
     2101 tggtgccata tctttacacc tgtgcggcgt tactactgct cggccacggt cactttggta
     2161 aagcacgccc ggcatacctg gcagttacta ccattgcctt cctctactgc atctgggccg
     2221 tagtagggtc cggcgcgaaa gaggttatgt ggtcatttgt cactctgatg gtaatcaccg
     2281 ccatgtatgc gctgaattac aaccggctgc ataaaaaccc gtatccctta gatgcaccaa
     2341 taagcaaaga ttaattcccc ttaatcaagc aaacataaaa gccaacctta agaacttaag
     2401 gttggcttaa ttttgctttg cgagcatatg cgcactttgt tcgatggaaa caccgtgatg
     2461 ttgaagcgcc tactaaaaag accctcttta aatttattct cctggttatt acttgctact
     2521 ttttatatct cagtctgcct gaatatcgcc ttttttaaac aggtgctaca ggtgttaccg
     2581 ctggattcac tgcataacgt gctggttttc ttgtcgatgc cggtcgtcgc attcagcgtg
     2641 attaatattg tcctgacact aggctctttc ttatggctta atcgtccact cgcctgcctg
     2701 tttattctgg tcggtgcggc tgcacaatat ttcatcatga cctatggcat cgtcatcgac
     2761 cgttcaatga tcaccaatat cattgatact acaccggcgg aaagttatgc gttgatgacg
     2821 ccacgaatgt tattaacgct ggggttaagt ggcgtgttgg ccgcgattat tgcctgctgg
     2881 atcaaaatca aacccaccac ctcacgcctg cgcagcgttc ttttccgcgg ggccaacatc
     2941 ctgatttctg tactgctgat tctgctggtt gccgcactgt tctacaaaga ctatgcctcg
     3001 ctgtttcgca acaacaaaga actggtgaaa tccttaagcc cgtccaacag cattgttgct
     3061 agctggtcgt ggtactctca ccagcggtca gccaacctgc cactgatacg cattggtgaa
     3121 gatgctcacc gcaacccatt aatgcaaaat gggaaacgca aaaacctgac cattttgatc
     3181 gtcggagaaa cgtcgcgcgc agagaatttc tcgctgaatg gttatccacg cgaaaccaat
     3241 cctcgcctgg caaaagataa cgtggtctat ttccctaata ccgcatcttg tggcacagca
     3301 acagccgtct cagtgccgtg catgttctcg gatatgccgc gtgagcacta caaagaagag
     3361 ctggcacagc accaggaagg cgtgctggat atcattcagc gagcgggcat caacgtgctg
     3421 tggaatgaca acgatggcgg ctgtaaaggc gtttgcgatc gcgtacctca ccagaacctc
     3481 accgcgctga acctgcctgg tcagtgcatc aacggcgaat gctatgacga agtgctgttc
     3541 cacgggctgg aagagtacat caataatctg caaagcgatg gcctcattgt attacacact
     3601 atcggcagcc acggcccgac ctattacaac cgctatccgc cgcagttcag gaaatttacc
     3661 ccaacctgcg acactaacga gatccagacc tgtaccaaag agcaactggt gaacacttac
     3721 gacaacacgc tggtttacgt cgactatatt gttgataaag cgattaatct gctgaaagaa
     3781 catcaggata aatttaccac cagcctggtt tatctttctg accacggtga atcgttaggt
     3841 gaaaatggca tctatctgca cggtctgcct tatgccatcg ccccggatag ccaaaaacag
     3901 gtgccgatgc tgctgtggct atcggaggat tatcaaaaac ggtatcaggt tgaccagaac
     3961 tgcctgcaaa aacaggcgca aacgcaacac tattcacaag acaatttatt ctcaacctta
     4021 ttgggcctga ctggcgttga gacgaagtat taccaggctg cggatgatat tctgcaaact
     4081 tgcaggagag tgagtgaatg aaaattctga ttgttgaaga cgatacgctg ttattgcagg
     4141 gattgattct ggcggcgcaa accgaaggct acgcgtgcga tggcgtgagc accgcgcgga
     4201 tggcggaaca aagccttgag gccggtcatt acagcctggt agtactggat ttaggcttac
     4261 ccgatgaaga tgggctgcat tttcttgccc gtatccggca gaaaaaatac actctgccgg
     4321 tgttaattct caccgcccgc gataccctta ccgacaaaat cgccgggctg gatgtcgggg
     4381 ccgacgatta tctggtgaag ccttttgcgc tggaagagtt gcacgcccgt atccgcgccc
     4441 tgctacgacg ccataataat cagggcgaaa gtgagctgaa cgttggcaac ctgacgctga
     4501 atatgggtcg ccgccaggta tggatgagcg gagaagaact gattctgacg cccaaagaat
     4561 atgctctgct gtcccgcctg atgcttaaag caggcagccc ggtgcatcgg gaaattctct
     4621 acaacgatat ctacaactgg gacaacgagc cctcgaccaa caccctggaa gtgcatatcc
     4681 ataacctgcg cgacaaagtg ggcaaagccc gtatccgcac cgtgcgcgga tttggctata
     4741 tgctggtcgc aaatgaggaa aactaattga atctgatgcg ttttctgcgc cgaccaatat
     4801 cgctgcgcca acggctgata ttgaccatcg gggccatttt gttggtgttt gagctgatca
     4861 gcgtcttctg gctatggcat gaaagtaccg agcagattca gctgtttgag caggcgctac
     4921 gcgacaatcg caacaacgat cgccacatca tgcgtgagat ccgcgaggcg gtcgccagcc
     4981 tgattgtccc cggcgtcttt atggttagcc tgacgctatt tatctgctat caggcggtac
     5041 gccgcatcac ccgaccgctg gcggagctgc aaaaagagct ggaagcgcgc accgccgaca
     5101 acttaacgcc cattgccatt catagcgcca ccctcgaaat cgatgcggtg gtttcggcgt
     5161 taaacgatct ggtcagtcgc ctgaccagca cgctggataa cgaaaggttg tttaccgctg
     5221 acgtcgcgca cgaactgcga acgccactgg cgggggtgcg tttgcatctg gaactgctgg
     5281 cgaaaacgca tcacattgat gtagcaccgt tagtggcacg acttgatcag atgatggaga
     5341 gcgtctccca gctgctgcaa ctggcgcgtg ccggacagtc attttcttcc ggtaattatc
     5401 aacatgtaaa actgctggaa gatgtcattc tcccctcgta tgacgaactc agtaccatgc
     5461 tcgaccagcg ccagcaaacc ctgctattgc cagagagcgc cgccgacatc accgttcagg
     5521 gtgatgcaac cctgctgcgg atgttattgc gaaacctggt agaaaacgcc catcgttaca
     5581 gcccacaagg cagcaacatt atgattaagc tgcaagaaga tggcggagcg gtcatggccg
     5641 ttgaagatga agggccgggt attgatgaga gtaaatgcgg ggagttgagt aaagcgtttg
     5701 tacgaatgga cagccgttat ggcggaattg gtctggggtt aagtattgtc agccgcatta
     5761 cacagttgca tcacgggcag tttttcctgc aaaaccggca agagacttcc ggcacgcggg
     5821 cctggatacg gctgaaaaaa gatcagtacg tggcaaacca gatataaaga aagctgctga
     5881 ccaccagcac gctgaacacg gtagttaaac tttcataaac acggtttttc attgcactct
     5941 cctccgttaa cctggaggag agtatgcgcg tcagagatta agtgaacctt aagagttcaa
     6001 ttgacctgat tagacagcgt cacatcaggc catccgtttc agcttattca tcaattcgcg
     6061 gatgttgctg caccaggcgg gtacgtttcg cctgcaaatc ggcaatctcg tggtcaatat
     6121 catcgatttt ctgctcgata ttatcgtaat gctcgacgag aatttccttc gcttcctgta
     6181 tatctgacgc cgctggtgtc gcacctttca acggtcgatt cgccgtctct ttcatggtta
     6241 cgccggtgat taaaccaacc accgccacta ccatcaggta ataggcaggc atcatcagat
     6301 tctgcgagct ttcgaccagc caggccgcca gcgttggcgt cagaccggca accagcaccg
     6361 aaatattaaa tgccgccgcc agcgcgctgt aacggatatg cgtcgggaac atcgcaggca
     6421 aggtagaagc cataacgccc gtaaagcagt taaggatcac cgccagcatc aataagccgg
     6481 caaaaatcag gccgatgacg ttactgttaa tcagaataaa cgccgggatc gccaacacaa
     6541 acaatgcaac actaccaaga agcacaaacg gacgacggcc aaaacggtca ctcagcaagc
     6601 ccatcaccgg ttgaacaaac agcataccga tcataatggc gataataatc agcaccccgt
     6661 ggtcttcgga gtaatgcagg ttatgcgaca aataactcgg catataggtc agcaacatgt
     6721 agtaagtcac gttggtggca attaccagac caatacatgt caacaggctg cgccagtgtt
     6781 tggtggcaat ctctttaaac gagactttcg ggccatcctg caaaccttcg cggtcgccct
     6841 gttccagttt atcgacatgc tgctggaacg ccggagtctc ttccagcgca tggcgcaggt
     6901 aaagcccgat aatccctaac ggcagagcga taaagaacgg aatacgccag ccccaatcga
     6961 ggaagttctc ttcgccgaca atggtcgaaa ttaacaccac tacgccagca cccagcacaa
     7021 acccggcaat agaaccaaag tccagccagc tgcccataaa gccacgttta cggtcagggg
     7081 agtattccgc aacaaatatt gacgccccgg tatattcacc gccgaccgag aaaccttgtg
     7141 ccatcttaca gatcaacagc agaatcggtg cccaaatacc aatcgtgtcg taggacggta
     7201 ttaagccaat acagaacgta ctgatcgaca taatcacaat agtgatagcg aggatcttct
     7261 ggcgaccata tttatcgccc aacataccaa agaagagccc gccaagcggt cgaatcagaa
     7321 agggaacgga gaaagtggcg agtgcagcaa ccatctgcac gctggggtca gcccccggga
     7381 aaaaaacttt acctaatgcg taagcaacaa aaccataaac accaaaatcg aaccattcca
     7441 ttgcattacc cagtgatgct gcggtaatgg ctttacgcag tttaccgtca tcaataatgg
     7501 tgacatcacg aagggtaatc ggttttactt ttttcctttt cagcatagct ttcctcgcag
     7561 agttggccca tttccgcaaa cgccagaaaa atcagcgcga cggccgcaaa aggggagcgg
     7621 gaattgcagg atgcaatctc tgtaacaagc gtagcgggtt tgcttacacc ctccggttaa
     7681 ggaatgtaca atcaaaccag ttgacgcctg aattgggcag ttaatgacct ttttacccta
     7741 ccagtgttta aatttgtgat caacttcaca catgcatttc gtttaggact cattggtgta
     7801 actgattatt tacaccaaat ttacagagaa gatttcctcg accattgccc ccgccagacg
     7861 ctgcgctgaa cagagtcgtg gcatccccag tgcgacagtt cgccagcgtt gggtcactga
     7921 ccagcaaacg ggttgtttat ctgccgtaga ccactttccc agccaactta aaaaatcagc
     7981 atgatccccg cccgtcgaaa cggtcgaaga ggcaaaccac tgatggtaat agtgacgcgt
     8041 ggtcggggcc gcattgatac tctctgccaa atgttgtgtc gctttcacgc gcaaatcacc
     8101 cagcaacaac gccaccgctt gcgggcttcc cagacggcag gcatcgccaa atgcacccca
     8161 gcgcaactcc tccagaacga taaagcagcg cccaggcaac gaccaggcgt tatacgcccc
     8221 agcacgccag cgggtaaaaa tttgctcgct ctgttcaccc gcctgcacca ataggcctcg
     8281 ttgcgttaac gccttttcct tccagcgaca acgggctgaa aaccactcat acaaggcgac
     8341 aatttgctgg gggagctccg ggaaagcaga gtgctggcgg tgatccattt tttgcagcag
     8401 cgccagatgc tgcgcgatct gcgtcagcgc cagatgaccg ggatccagca tcgacgccag
     8461 tttctccacc aatgtcagac gcagactaag cggcgcattt aacatgccgc cgttcgtcca
     8521 tgtctgaagc tgcacgcgcg caagaagttc ctgcatcagt cgttcacgaa actgctgctg
     8581 atgggcttgt ggaaggcggg catcatcgcc ctgcgccaga tccactaaaa agcgcggatg
     8641 aaccgactcc agcacgcgac cggggccgtc cagtaacgtc ttggtcaata tcgttctgcc
     8701 gtgaaaagtg tttgaatatc atcgcgtaac agctgagcgt cggtgtaaat ccagccgtga
     8761 gtcatcacag tctgctgcaa ttgctgttgc atcagcctga ccaccgattc attttgttga
     8821 cgcagagcca gactttcgcg taaacgcgtc tgtaattccg tcaaacatga agcgaactca
     8881 gcgaaaaaag tattcatgcc tgccgtaaca gattcatcga cctgctctgc cagaacttta
     8941 gccatttgtt ggcaataaag atcgacttct gcgcttaatg ctcgctgcaa cacactgtaa
     9001 tcaaccgtct ctgtcgagaa tttctcattt ccccgtcccc agtcgggctg attcaaccag
     9061 cgcgaaaaaa tctcacgcac aacgcctaaa cgcgtgctct gctcgtccgt tgctgcctgg
     9121 cgcgaaatga cttcactgaa cagctggcgg gtgttgaagt ggggaactac gccgtgaaaa
     9181 acaggaaaat gaaacccagg acgaaaccca gactcactca attccatttt gacttgttgc
     9241 tcaatggggc gaataacatc ggttaacacc cggcaaaggg tggactcaag ctcggcaaag
     9301 cgcagcgtaa agtcgcgact gatggtgttc tgcgccgtct gtaacagtgt ctcacagcgg
     9361 gtacgcatct cacttaacga ctccgaatca tcctgaaaca acgcggctaa ctgcgcattc
     9421 accgcatctt gctgttgacg cagaaagtgg ttggtggagg tcagggccaa ctcgatttca
     9481 tgtttaatct cgccgctcac ctgcgcctga ttgagttgca atagctgcaa actttcttcg
     9541 acctgatgga tattttgccg caattgttca caagcgacgt ttaacccgtg cgcacgaaaa
     9601 tccaggtatt cccgcgcctg ctgcgcgtaa ttcaacagtt tatgcgcggc agatcgcaga
     9661 gcatacaacg aggcgttagc gtaagcggca tgaagcaacg cctgaattgg ctgggcgaac
     9721 agcgaatctt cccacaactg atcggcagca tggcgaatat gttcgaggtc cgccagatcg
     9781 gcatgacgcc agcgcctgcc gagcgcggca tgggcaaaat cttccaccca gcgttgttgc
     9841 tctggcggtg gtaacttacc gttgttggct aactcatggc gcgcccgatt cgccaggtag
     9901 ccccacatcg acgacaccgg aaatatctgc tgtggcgtaa tacagccttt catcagcgtt
     9961 ccggaaatca gtgcccgcac ctggtcggcg tcgtcactgt tacgatcctg ttgatcgaac
    10021 ttattgacca gcacatacaa cggcaccgat tgccccaccg ccaaaattgc ctcacggacc
    10081 tcttcatcgg agatcgattt cagttgcgta taatccagca ccgccagtac cgccgaggcg
    10141 cgtgccagct gctggttaag cattttttgc agatgcggtt gcccggcttc atttggcccg
    10201 ggggtatcca gtaacgtcaa ctgaccggga taactctcca gccccgccag atggacaaac
    10261 tccacttcaa tcacgggaat atgctcaatg gcggcgtaag cagaaaaagg aaaatcgacg
    10321 tccagcgcct tcgccagtcg caccaaatca ttcaaacttt tcagacaatg aaaaataggt
    10381 tgagcaccca gataatattt ttcgaaagcg atgccatttt caatccgctg cataagcgca
    10441 cgcatatctt tatctatttc cagcacatcg gtcagatgct taatatcgca atcacgcagg
    10501 cgctgttgta attgttgaat taaatggtcg attggcgcga catgtgaaaa atgcagtacc
    10561 ggttcttttt gcccgggcgt atggcgaata agcgttggca gcgcagtcat tgggcgattg
    10621 cgattaggca gaacctccgt accaacaatg gcattaatgg tggttgattt ccctgctttc
    10681 atggtaccga caatcgccag caccatttcc agctgggaaa ttttacgcaa ctcattattc
    10741 agcatcgcgt gacgttcggc gatattaggc tgactccagg gtaaagccag ttgtggcgca
    10801 tcgtctccgg gtacagagag aggtattttt tccagtaact gcaactgttg gcgagaaagc
    10861 tgtaacaggc gttcagcctc ctgacttaac tcatacaggg tctgtgtgta catagaatat
    10921 tcttccttaa agcaaatttt attactttta tttagccaga t
//
LOCUS       DAZFJL010000151        10932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZFJL010000151 DAZFJL010000000
VERSION     DAZFJL010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.153"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            109..1251
                     /gene="yghO"
                     /locus_tag="ACHJ8C_003985"
     CDS             109..1251
                     /gene="yghO"
                     /locus_tag="ACHJ8C_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO5553764.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     SEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNFGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAITDLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1258..2022)
                     /gene="glcC"
                     /locus_tag="ACHJ8C_003986"
     CDS             complement(1258..2022)
                     /gene="glcC"
                     /locus_tag="ACHJ8C_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO5553765.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDASPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHMRTVKKNLHDIELEGHHLIRSAVPLEMKQG"
     gene            2273..3772
                     /gene="glcD"
                     /locus_tag="ACHJ8C_003987"
     CDS             2273..3772
                     /gene="glcD"
                     /locus_tag="ACHJ8C_003987"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO5553766.1"
                     /translation="MSILYEERLDGALPEVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPIGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQMLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLNAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRIANVFHAGDGNM
                     HPLILFDANEPGEFVRAEELGGKILELCVEVGGSISGEHGIGCEKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3772..4824
                     /gene="glcE"
                     /locus_tag="ACHJ8C_003988"
     CDS             3772..4824
                     /gene="glcE"
                     /locus_tag="ACHJ8C_003988"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO5553767.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTSLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRATLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVADQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4835..6058
                     /gene="glcF"
                     /locus_tag="ACHJ8C_003989"
     CDS             4835..6058
                     /gene="glcF"
                     /locus_tag="ACHJ8C_003989"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO5553768.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARLVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6063..6467
                     /locus_tag="ACHJ8C_003990"
     CDS             6063..6467
                     /locus_tag="ACHJ8C_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO5553769.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6489..8660
                     /gene="glcB"
                     /locus_tag="ACHJ8C_003991"
     CDS             6489..8660
                     /gene="glcB"
                     /locus_tag="ACHJ8C_003991"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO5553770.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPDHVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDTAAPTCILLKNNGLHIELQIDANGRIGKDDSAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFSRVETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9015..10697
                     /gene="glcA"
                     /locus_tag="ACHJ8C_003992"
     CDS             9015..10697
                     /gene="glcA"
                     /locus_tag="ACHJ8C_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFO5553771.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
     gene            complement(10725..>10932)
                     /locus_tag="ACHJ8C_003993"
     CDS             complement(10725..>10932)
                     /locus_tag="ACHJ8C_003993"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553772.1"
                     /translation="IAKATDRTVFAHQANIINTDLFCAIKNRCSHALNYLACYLNAKY
                     YCCMDNKVGNNFPLIRQSNVNEAG"
BASE COUNT         2538 a         2724 c         3068 g         2602 t
ORIGIN      
        1 taaatactat ttattctttg tttgtattca ttgggttgat tattaagatg ccgttcgttg
       61 ctgttgcaac ctgtttttca ccgttaatta tttgttatcc acagagatat ggaatgcgat
      121 ctgctgatga ttaaaattga aaaagtaatt aataaaaatg accttaaggc atttatcgct
      181 tttccgtcat cactttatcc cgatgatcca aactggatac cccctttatt cattgaacgc
      241 agcgagcatt tgtctgcgaa gaatccgggg acggaccata tcatctggca ggcgtgggtg
      301 gcaaaaaaag cggggcagat agttgggcgt attaccgcgc aaatcgatac cttgcaccgt
      361 gagcgttacg gtgaagatac cggtcatttc ggaatgattg atgccattga tgatccgcag
      421 gtttttgccg cgttgtttgg cgcagcggag gcgtggttga agtcacaagg tgcaagtaag
      481 atcagcgggc ctttcagcct gaatatcaat caggaaagcg gattactgat tgaaggtttt
      541 gacacacctc cctgtgcgat gatgccacac ggcaaaccgt ggtatgccgc gcatattgaa
      601 caactgggtt atcacaaagg cattgattta ctggcgtggt ggatgcagcg aaccgatctc
      661 actttctctc cggcgctaaa aaaactgatg gatcaggtgc gcaaaaaagt gaccattcgc
      721 tgcatcaatc gccagcggtt tgccgaagag atgcagatcc tgcgtgagat tttcaatttt
      781 ggctggcagc acaactgggg atttgtgccg tttaccgaac atgaattcgc gaccatgggc
      841 gatcaactta aatatctggt gccggacgat atgatctaca tcgctgagat tgattctgca
      901 ccctgcgcgt ttattgttgg cttgccgaac atcaacgagg cgattaccga tctgaacgga
      961 tcgctctttc ccttcggctg ggcaaaattg ctgtggcgtt tgaaagtcag cggtgtgcga
     1021 actgcgcgag tgccgctgat gggcgtgcgg gacgagtatc agttcagccg catcggcccg
     1081 gtgattgcgc tgttattgat tgaagcctta cgcgatccgt ttgcccgccg gaagatcgat
     1141 gcgctggaaa tgtcatggat cctcgaaacc aatacaggca tgaataacat gctggaacgc
     1201 attggggcgg agccgtacaa gcgttaccga ttgtatgaaa agcagatttg atggcagtca
     1261 cccctgcttc atctccagcg gcaccgccga gcgaatcaaa tggtggcctt ccagctcgat
     1321 atcgtggaga ttctttttca cggtccgcat atgatcgcgt gctgcgcgct gggcaacgtg
     1381 cggcagccgc tgcaacaccg cgttgtagat ccgcgcatgc tggcgatcga tctgcttttt
     1441 ttgctgtggt cgatggtaga gattatttac gctggcaaac accgaattaa acatcaggtc
     1501 ggtcaatgat tgcagcgtaa acaccagcac ctgattgtga gaagcctgac aaatggcgag
     1561 atggaaagcg tgatccaact gcgcatgttc gatcagcgaa atctctttgt tgttctcact
     1621 ggcggcgagc attttttcat aacagcgggt tatcacaaca aaatcagcct gggttcccag
     1681 cgttgccgcc agccttgccg attcgccctc cagtaatgcg cgaacgtcga gcagatcgta
     1741 cagcgttcgc ggctgcgtac tgaacagatg gatcagcggg ctggcgtcct gcacccgatt
     1801 aagccgtgcg acacgagaat cgcgaccctg cgccgtttca ataatcccgc gcccgcgcag
     1861 cacggtcagc ccttcacgca gtgcggagcg tgagaagccg agcttttcac acagccgacg
     1921 ttccgaggga agcggctgac cgaccttcaa cacgccgtcg ataattaacc gttcgatact
     1981 ctctgcaacc acttcgcaaa tagggcgacg ttcatctttc attcccggac ctcgtgcaca
     2041 ggtaggacca atttttctgt tcttaactcg caaaacacgc acatcacgta agtgattatg
     2101 ttacatcaat ttaacattaa gttaaccaaa gcaaggtcac agagctggaa aaaaaatggt
     2161 ctgaccggta gctaaagaga tagacgaaaa cgaaaagccc gcttaataac tgttcacaga
     2221 agcagcgcga aaaaatcagc tgccacacaa cacaacaaag cgaaccctac tcatgagcat
     2281 cttgtacgaa gagcgtcttg atggcgcttt acccgaagtc gaccgcacat cggtactgat
     2341 ggcactgcgt gagcatgtcc ctggactgga gatcctgcat accgatgagg agatcattcc
     2401 ttacgagtgt gacgggttga gcgcgtatcg cacgcgtcca ttactggttg ttctgcctaa
     2461 gcagatggaa caggtgacgg cgattctggc tgtctgccac cgcctgcgtg tgccggtggt
     2521 gacccgtggc gcaggcaccg ggctttctgg tggcgcgctg ccgctggaaa aaggtgtgtt
     2581 gttggtgatg gcgcgcttta aagagatcct cgacattaac cccatcggtc gccgcgcgcg
     2641 cgtgcagccg ggcgtgcgta acctggcgat ctcccaggcc gttgcgccgc ataatctcta
     2701 ttacgcgccg gatccgtcct cacaaatcgc ctgttcgatt ggcggcaatg tggcggaaaa
     2761 tgccggtggc gttcactgcc tgaaatatgg tctaaccgta cataacctgc tgaaaattga
     2821 agtgcaaatg ctggatggcg aggcgctgac gctgggatcg gacgcgctgg attcgcctgg
     2881 ttttgacctg ctggcgctgt ttaccggatc ggaaggtatg ctcggcgtga ccaccgaagt
     2941 aacggtgaag ctgttgccga agccgcctgt ggcgcgggtt ctgttagcca gctttgactc
     3001 ggtagaaaaa gccggacttg cggttggtga catcatcgcc aatggcatta tccccggtgg
     3061 gctggagatg atggataacc tgtcgattcg cgcggcggaa gattttattc atgccggtta
     3121 tcccgtcgat gccgaagcga ttttgttatg cgaactggac ggcgtggagt ctgacgtaca
     3181 ggaagactgc gagcgggtta acgacatctt gttgaacgcg ggtgcgactg acgtccgtct
     3241 ggcacaggac gaagcagagc gcgtacgttt ctgggccggt cgcaaaaatg cgttcccggc
     3301 ggtaggacgt atctccccgg attactactg catggatggc accatcccgc gccgcgccct
     3361 gcctggcgta ctggaaggca ttgcccgttt atcgcagcaa tatgatttac gcattgccaa
     3421 cgtctttcat gccggagacg gcaacatgca cccgttaatc cttttcgatg ccaacgaacc
     3481 cggtgaattt gtccgcgcgg aagaactggg cgggaagatc ctcgaactct gcgttgaagt
     3541 tggcggcagc atcagtgggg aacatggcat cgggtgcgaa aaaatcaatc aaatgtgcgc
     3601 ccagttcaac agcgatgaaa tcacgacctt ccatgcggtc aaggcggcgt ttgaccccga
     3661 tggtttgctg aacccaggaa aaaacattcc cacgctacac cgctgtgctg aatttggtgc
     3721 catgcatgtg catcacggtc atttaccttt tcctgaactg gagcgtttct gatgctacgc
     3781 gagtgtgatt acagccaggc gctgctggag caggtgaatc aggcgattag cgataaaacg
     3841 ccgctggtga ttcagggcag caatagcaaa gcctttttag gtcgccctgt caccgggcaa
     3901 acgctggatg tgcgttgtca tcgcggcatt gttaattacg acccgaccga gctggtgata
     3961 accgcgcgtg ccggaacgtc gctggtggcg attgaagcag cgctggagag cgccgggcaa
     4021 atgctcccct gcgagccgcc gcattatggt gaagaagcca cctggggcgg gatggtcgct
     4081 tgcgggctgg cggggccgcg tcgcccatgg agtggttcgg tccgcgattt tgtcctcggc
     4141 acgcgcatca ttaccggcac tggaaaacat ctgcgttttg gtggcgaagt gatgaaaaac
     4201 gttgccggat acgatctctc acggttaatg gccggaagct acggttgtct gggtgtgctc
     4261 actgaaatct cgatgaaagt gttaccgcga ccacgcgcca ccctgagtct gcgtcgggaa
     4321 atcagcctgc aagaagccat gaatgaaatc gcccagtggc aactccagcc attacccatt
     4381 agtggcttat gttacttcga caatgcgttg tggatccgcc ttgagggcgg cgaaggatcg
     4441 gtaaaagcag cgcgtgaact gctgggtggc gaagaggttg cggatcagtt ctggcagcaa
     4501 ttgcgtgaac aacaactgcc gttcttctcg ttaccaggta ccttatggcg catttcatta
     4561 cccagtgatg cgccgatgat ggatttaccc ggcgagcaac tgatcgactg gggcggggcg
     4621 ttacgctggc tgaaatcgac agccgatgac aatcaaatcc atcgcattgc ccgcaacgct
     4681 ggcggtcatg cgacccgctt tagtgccgga gatggtggct ttgccccgct accggctcct
     4741 ttattccgct atcaccagca gcttaaacag cagctcgacc cttgcggcgt gtttaatccc
     4801 ggtcgcatgt acgcggagct ttaaggagca ggctatgcaa acccaattaa ccgaagagat
     4861 gcggcagaac gcgcgggcgc tggaagccga cagcatcctg cgcgcctgtg ttcactgcgg
     4921 attttgtacc gcaacctgcc caacctatca gctcctgggc gatgaactgg acgggccgcg
     4981 cgggcgcatc tatctgatta aacaggtgct ggaaggcaac gaagtcacgc ttaaaacaca
     5041 ggagcatctc gatcgctgcc tcacttgccg caattgtgaa accacctgtc cttctggtgt
     5101 gcgttatcac aatttgctgg atatcgggcg tgatattgtc gagcagaaag tgaaacgccc
     5161 actgccggag cgaatgctgc gcgaaggatt gcgccaggta gtgccgcgtc cggcggtctt
     5221 ccgtgcgctg acgcaggtag ggttggtgct gcgaccgttt ttaccggaac aggtcagagc
     5281 aaaactgcct gctgaaaccg tgaaagctaa accgcgtcca ccactgcgcc ataagcgtcg
     5341 ggttttaatg ctggaaggct gcgcccagcc tacgctttcg cccaacacca acgcggcaac
     5401 tgcgcgagtg ctggatcgtc tggggatcag cgtcatgcca gctaacgaag caggctgttg
     5461 tggcgcggtg gactatcatc ttaatgcgca agagaaaggg ttagcgcggg cgcgcaataa
     5521 tattgatgcc tggtggcccg cgattgaagc aggtgccgag gcaattttgc aaaccgccag
     5581 cggttgtggc gcgtttgtca aagagtatgg gcagatgctg aaaaacgatg cgttgtatgc
     5641 cgataaagcg cgtctggtca gtgaactggc ggtcgattta gtcgaacttc tgcgcgagga
     5701 accgctggaa aaactggcaa ttcgcggcga taaaaaactg gccttccact gcccgtgtac
     5761 cctacaacat gcgcaaaagc tgaacggcga agtggaaaaa gtgttgcttc gtcttggatt
     5821 taccttaacg gacgttcccg acagccatct gtgctgcggt tcagcgggaa catatgcgtt
     5881 aacgcatccc gatctggcac gccagctgcg ggataacaaa atgaatgcgc tggaaagcgg
     5941 caaaccggaa atgatcgtca ccgccaacat tggttgccag acgcatctgg cgagcgccgg
     6001 tcgtacctct gtgcgtcact ggattgaaat tgtagaacaa gcccttgaaa aggaataaca
     6061 aaatgaaaac taaagtcatt cttagccagc aaatggcgag tgcaattatt gccgcaggtc
     6121 aggaagaggc gcagaaaaat aactggtctg tttccattgc tgttgccgat gacggcggtc
     6181 atctgctggc gttaagtcgc atggacgatt gcgcgccgat tgcggcttac atctcccagg
     6241 agaaagcgcg taccgccgcg ctggggcgtc gtgaaactaa gggctatgaa gagatggtga
     6301 acaacggacg taccgcgttc gtgactgcgc cgttactgac gtcgctggaa ggtggcgtac
     6361 cggttgttgt ggatgggcaa attattggtg ccgtgggagt ttctggttta accggagcac
     6421 aggatgcgca ggtcgcgaaa gcggcagcag cggtgttggc gaaataagcg aaaacgagga
     6481 gataaacaat gagtcaaacc ataacccagg gccgtttacg cattgacgcc aattttaaac
     6541 gttttgtgga tgaagaagtt ttgcctggcg tggaactgga tgctgccgcg ttctggcaca
     6601 atgttgatga gatcgttcac gatctggcgc cagagaatcg tcagttgctg gcagagcgcg
     6661 atcgcattca ggcggcgctt gatgagtggc atcgcagcaa tccggggccg gtaaaagata
     6721 aagcggccta taaatctttc ctgcgtgaac tgggctacct ggtgccacag cctgaccacg
     6781 ttacggtgga aaccacgggc atcgacagcg aaatcaccag tcaggcgggg ccacagctgg
     6841 tggttccggc gatgaacgcc cgctacgcgc tgaacgcggc gaacgctcgc tggggctcac
     6901 tgtacgatgc gttatacggc agcgacatca tcccgcagga aggggcgatg gtcagcggct
     6961 acgatccgca gcgtggtgag caggttatcg cctgggttcg gcgtttcctc gatgaatcac
     7021 taccgctgga aaatggcagc tatcaggatg tggtggcgtt taaggtggtc gataaacaat
     7081 tacgcatcca gttgaaaaat ggtaaagaaa ccacgttacg tactccagca cagtttgtcg
     7141 gttaccgtgg cgatactgct gcgccgacct gcattttgct gaaaaataac ggcctgcata
     7201 ttgagctaca aatcgatgcc aacgggcgga ttggcaaaga cgattcggcg cacatcaacg
     7261 atgttatcgt cgaagcggcc atcagtacca ttctcgactg cgaagattcg gttgcggcgg
     7321 ttgatgcgga agataaaatc ctgctgtacc gcaacctgct gggcctgatg caggggactc
     7381 tgcaagagaa aatggagaag aacggtcggc aaatcgtgcg taaactgaat gacgatcgtc
     7441 agtacactgc cgccgatgga tctgaaattt ctctgcacgg acgctcgttg ctgtttatcc
     7501 gcaacgtggg tcatttgatg accattcctg ttatttggga cagcgaaggc aatgaaatcc
     7561 cggaaggcat tcttgatggc gtcatgactg gcgcgattgc cctctatgat ttaaaagtgc
     7621 agaaaaactc gcgcactggc agcgtctata ttgtgaaacc gaaaatgcac ggtccgcagg
     7681 aagtggcgtt cgccaacaaa ctgttttccc gcgttgagac aatgctcggt atggcaccga
     7741 ataccctgaa aatgggcatt atggatgaag aacgccggac ctcgctgaac ttgcgtagct
     7801 gtatcgctca ggcgcgcaac cgcgtggcgt tcatcaatac cggtttcctc gaccgtaccg
     7861 gcgatgaaat gcattcggtg atggaagcgg gcccgatgct gcgtaaaaac cagatgaaat
     7921 cgacgccgtg gatcaaagcc tacgaacgta ataacgtgct ttccggtctg ttttgcgggc
     7981 tgcgtggtaa agcgcaaatc ggtaagggga tgtgggcaat gccggacctg atggcagaca
     8041 tgtacagcca gaagggcgac caattgcgtg ccggggcaaa cacggcctgg gttccgtcgc
     8101 caactgccgc tacgctccat gcgctgcact accaccaaac caacgtacag agcgtgcaag
     8161 ccaacattgc ccagaccgag ttcaatgctg aatttgaacc gctgctggac gatctgctga
     8221 ctattccggt tgctgaaaac gctaactggt cggcgcaaga gatccaacaa gagctggata
     8281 acaacgtgca ggggattctg gggtacgtgg tgcgttgggt ggagcagggg attggttgtt
     8341 caaaagtgcc ggatattcac aacgtggcgt tgatggaaga ccgcgcaacg ctgcgtatct
     8401 ccagccagca tatcgccaac tggttacgtc acggtattct gaccaaagaa caggtgcagg
     8461 cgtcgctgga gaatatggcg aaagtggttg atcagcaaaa cgctggcgat ccggcttatc
     8521 gtccgatggc ggggaatttc gctaactcgt gtgcttttaa agctgccagc gatttaatct
     8581 tcctcggcgt gaaacagcca aacggctata ccgaaccgtt attacacgcc tggcgtttac
     8641 gcgaaaaaga aagtcattaa tagcgtcaaa aaagccccgg caagttttat tgtcggggct
     8701 atgatagtaa ctgcggtagt acatttatcc cggaaataac cggagcgatt atcagggttt
     8761 aacaatatta aatacccgta gcgcattact agcgggccta aaacaacaat atggacatct
     8821 taacgatatt ctgcgcctgc gtgtgcggaa cagaaaccct tacccgatat acttgttatg
     8881 ccagaggtag caattgcgtt agcagcgtac tgttgctgta aagtataacg cctatatttt
     8941 acagttcagt cctcttttgc ttattacaac cttaagtata tcaagcatat aaagataata
     9001 agagactgaa caatatggtt acctggaccc aaatgtatat gccgatggga ggactggggc
     9061 tatccgctct ggtcgccctg atcccgataa tattcttctt cgttgcactc gcggtattac
     9121 gtctgaaagg acatgtcgct ggagcaataa cccttatatt atctatcctg attgcaatat
     9181 tcgcctttaa aatgccgatt gatatggcat ttgctgctgc gggctatggc tttatttatg
     9241 gattatggcc tattgcgtgg attattgttg cggcggtgtt cctctataaa ttaaccgttg
     9301 ccagcggtca gttcgatatt atccgcagct cggttatctc catcactgac gatcagcgtt
     9361 tacaggtgtt actgattggt ttctcctttg gcgcgctgct ggaaggggcg gctggctttg
     9421 gtgcgccggt ggcgattacc ggtgcgctgc tggtgggcct gggcttcaaa ccgttatacg
     9481 cggcggggct gtgtctgatt gccaatactg cgccggtggc gtttggtgcg ttgggcgtgc
     9541 cgattctggt cgccggtcag gtaacgggaa tcgatccgtt ccacattggc gcaatggcgg
     9601 gacgtcagtt accgttcctg tcggttcttg tgccgttctg gctggtagca atgatggacg
     9661 gctggaaagg ggtgaaagag acgtggccag cggcgctggt tgctggggga agcttcgctg
     9721 tcacgcagtt ctttacctct aactatattg gtccggaact gccggatatt acttcggcgc
     9781 tggtgagtat cgtctcactc gctttattcc ttaaagtctg gcggccgaaa aataccgaaa
     9841 cggcaatcag catgggacaa tccgcaggag cgatggtggt gaataaacca tcttctggcg
     9901 gtcccgtgcc ttcagaatat agtctggggc aaatcattcg tgcgtggtca ccgtttttaa
     9961 tcttaacggt gctggtcacc atctggacca tgaagccgtt taaagcgtta tttgctccgg
    10021 gcggcgcgtt ttattcactg gtgattaatt tccagatccc tcatttgcat caacaagtgt
    10081 tgaaagcggc acccattgtc gcccaaccaa cgccaatgga tgcggtgttt aaattcgacc
    10141 ccctctctgc tggtggcacc gctattttta ttgcggcgat tatctcgatc ttcatcctcg
    10201 gcgtggggat caagaaaggt attggtgtct ttgccgaaac gctaattagt ctgaagtggc
    10261 cgatactgtc gattggcatg gtgctggcat ttgccttcgt gaccaactat tccggcatgt
    10321 ccaccacgct ggcgctggta ctggcaggta caggcgtgat gttcccgttt ttctcaccgt
    10381 ttctcggctg gctgggcgta ttccttaccg gctcggacac ctcctctaac gccctgtttg
    10441 gttcactgca atcgaccacg gcgcagcaaa tcaacgtctc tgacactctg ctggtggcag
    10501 caaacaccag cggcggcgta actggcaaga tgatctcccc gcaatctatc gccgtggcct
    10561 gcgccgcgac gggcatggtg ggccgagaat ctgaactgtt ccgctacacc gtgaagcaca
    10621 gtctgatttt tgccagcgtt atcggcatta tcacgctgtt gcaggcgtat gtgtttactg
    10681 ggatgttagt ttcgtaagtc aataaagccg gatgatcgtt aatatcatcc ggcttcgttt
    10741 acattactct gtcttattaa tgggaaatta tttcccactt tattatccat gcagcaataa
    10801 tattttgcgt tcagataaca tgctaaataa ttaagagcgt gagaacatct attttttatc
    10861 gcgcaaaaca agtctgtatt aattatgttc gcttgatgag caaacaccgt tctatctgtt
    10921 gcttttgcaa tc
//
LOCUS       DAZFJL010000152        10913 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFJL010000152 DAZFJL010000000
VERSION     DAZFJL010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.154"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            261..1154
                     /locus_tag="ACHJ8C_003994"
     CDS             261..1154
                     /locus_tag="ACHJ8C_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5553773.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGMPSGNIRLSAGE
                     HAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIESGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(1185..2174)
                     /locus_tag="ACHJ8C_003995"
     CDS             complement(1185..2174)
                     /locus_tag="ACHJ8C_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO5553774.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGNDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLHRLEENLAAADIVLSQKDTQQISEALETIKIVGERYSPEHQARVGR"
     gene            complement(2201..3052)
                     /locus_tag="ACHJ8C_003996"
     CDS             complement(2201..3052)
                     /locus_tag="ACHJ8C_003996"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO5553775.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINSGYRLID
                     TAASYQNEIQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVLLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            3617..7867
                     /gene="fdeC"
                     /locus_tag="ACHJ8C_003997"
     CDS             3617..7867
                     /gene="fdeC"
                     /locus_tag="ACHJ8C_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFO5553776.1"
                     /translation="MSRYKTDNKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMENTTVTADNNVEKNVASLAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKNFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSENDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTVSLGLVVSKATHGLK
                     NVQWEAPSLLAAGGKITGQGNQWQVTLPAYQAGKDNYYAISAIAYDNKGNASKRVQTE
                     VVISGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDDMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAAGSTASGW
                     TDNGDGTWTAQISLGTTAGELDVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGELEIEASVKNSQKTVKVKF
                     KADFSTGQASLEVDAAAQKVANGKDAFTLTATVKDQYGNLLPGAVVVFNLPRGVKPLA
                     DGNIMVNADKEGKAELKVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSEVTLTASPENLVLTPNGTATTNEQGQA
                     IFTATTTVAATYTLTAKVEQADGQESTKTAESKFVADDKNAVLAASPERVDSLVADGK
                     TTATLTVTLMSGVNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRTFSTNK
                     PGTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
     gene            complement(7992..8849)
                     /locus_tag="ACHJ8C_003998"
     CDS             complement(7992..8849)
                     /locus_tag="ACHJ8C_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5553777.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLSAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKLPDEEVKL
                     RRHHLVRCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            9097..9966
                     /locus_tag="ACHJ8C_003999"
     CDS             9097..9966
                     /locus_tag="ACHJ8C_003999"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO5553778.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKDVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(10126..10719)
                     /gene="rclC"
                     /locus_tag="ACHJ8C_004000"
     CDS             complement(10126..10719)
                     /gene="rclC"
                     /locus_tag="ACHJ8C_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO5553779.1"
                     /translation="MERYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQHLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSARDILKQRSNESSSTLKTEY"
     gene            complement(10731..>10913)
                     /gene="rclB"
                     /locus_tag="ACHJ8C_004001"
     CDS             complement(10731..>10913)
                     /gene="rclB"
                     /locus_tag="ACHJ8C_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO5553780.1"
                     /translation="STNADDKIILKHISVSSVSASPTVLENAIADMARKYNASSWKVT
                     SMRIDNNSTATAVLYK"
BASE COUNT         2914 a         2456 c         2772 g         2771 t
ORIGIN      
        1 ctctaaagtt cgtgctttta ttgattttat ggtggagaaa agagtaaaaa tagaaagtta
       61 ataatgtgtg tcagaatatc ggtaagaaga atggcgcaat cgtgaggata cgtttattca
      121 taataaatct cctgaaagtt tagaattatt ttcgatacag ccagattatc attgttacta
      181 ataatgtggt taaaataata gcatctatgc cccccttcat gagtgtctgt gaagaggatg
      241 cttctacaag gaaatcgcca atgctcaaag aaaacttcaa cgaactgcaa atctttcttg
      301 tggtggcaag agagcgaagt tttaccaaag cagcgggcaa actcggcgtc tctcagtctg
      361 cacttagcca tgcaatgaag gctctggagg aaaggctcaa tatccgcctc ctgacacgga
      421 ctacccgcag tgtggctccc acagaagcgg gggagagaat cattgcctgc cttgaaccgc
      481 gtattgacga gcttgaacag gaactggaat cacttattca attgaacggc atgccctccg
      541 ggaatattcg gttatctgcc ggggaacatg ccgcgcgaag tctggtctgg ccgaagctaa
      601 aaccttttct cagagagtat cccgaaatca atgtcgaact ggtggtggat aatggttttg
      661 tcgatattgt tgaaggccgt tttgatgccg ggatacgact gggtgaaaat gttgataaag
      721 atatggttgc agtcaggatt ggaccagata tgcggatggc tgtggtggga gctccggcgt
      781 attttgctgc aaaccctgcg cctgaaacgc ctcatgagct acaaaatcat cgctgcatca
      841 acatgcgttt gccaactgcc ggtgggcttt accactggga gttcgagcga gaagggaaac
      901 cgcttcgcgt taaggttgat gggcaactga cgtgtagtcc attgccagaa agaattgatg
      961 ccgcgttatc agggtttggg atcgcttgtg tccctgaaga catggttcag gagtatatag
     1021 agtcaggcaa gcttattcag gtgttacagg agtggtgtcc aacttttcct gggtattatc
     1081 tttactaccc cagccgcaag cagcatccgc cagcttttgc gctgttgatc gatgcgcttc
     1141 gctacaccga ataacgggtc ctttatcctg gacccgtttg ggtattagcg gcctacgcgt
     1201 gcctgatgtt caggcgagta acgttcaccg acgattttaa ttgtttccag cgcctcgctt
     1261 atctgctgcg tatctttctg cgaaagaaca atgtcagcag cggccaggtt ttcttccagc
     1321 cggtgcagtt tagtggtgcc agggatggga acaatccacg gcttttgtgc taatagccat
     1381 gccagtgcga tttgtgcaga cgttacccct ttctccgcag ccagttcacc taacaaggtg
     1441 accagttttt cattggcttc aatggcttgt gctgcgaaac gcggtacggt gctgcggtaa
     1501 tcatctttgc cgaaagtggt tcccggctta atcgcccctg tcaggaaacc tttacctagt
     1561 gggctgaagg ggacaaaacc aattcccagt tcctcaagca acggcagaat ttcctgttca
     1621 ggctcacgcc accacatgga atattcgctt tgcagggcgg tgacaggttg taccgcatgt
     1681 gcacgacgaa tggtttgtgc tcctgcttca gacaggccga aatgtttgac cttaccttct
     1741 gcgatgaggt ctttcaccgt tcctgcaaca tcttcaatag gaacatctgg atcaacacgg
     1801 tgttgataca gcaggtcaat aacatctgtt ttcagacgac gtaatgatcc ttcaacggct
     1861 tcacggatat gttccggacg gctgtttaaa atctgctgct tattatcatt gccgaaggta
     1921 aaaccgaatt tagtggcaat taccacgcgg tcacgaaacg gttttaaggc ttcgccgacc
     1981 acttcttcat tcagatacgg accatacact tcggcggtat cgaagaaggt gacgccacgt
     2041 tcaaccgcag cacgaataag ttcgatagcc tgacgggtat cggtcgccgg gccgtagccg
     2101 tggcttaagc ccatgcaacc gagcccaagg gcggagactt cgagtccgga tttacccaga
     2161 taacgttttt gcattgaatg aatacctctt ttatcgcggc ttaaacatca agtttgcggc
     2221 cagtcagcca ttccaccatt gcgggatcgc ggtgagagaa gaacgcgctg gttgcggtat
     2281 ccagggcggt aatttgcagc atatcttcag ggctgagttc aaaatcgaga atgttgatgt
     2341 tctcttccat gcgttctttt cgcactgatt ttgccagtaa aacaatgcct cgttggtaga
     2401 tccagcgcag aactacctgg cccacgcttt tgccatactt ctcgccaatt gccgtgagaa
     2461 caggatgctg gaacagacca tttttccctt ccgcaaacgg tgcccaggct tctggctgaa
     2521 tgccacgact ttgcatccag ggaacggcct gtagctgctg attgaaaggg ttaacttcaa
     2581 cctggtttac cgccggaacg acgttattaa aagcgataag gtctgccagt cggtcaggat
     2641 ggaagttact gacgccaata gcgcgaattt taccggcctg ttgcagctct tccatggcac
     2701 gccaggcccc gtggacatcg ccatatggct ggtgaatcaa gtacaggtca acgtaatcaa
     2761 gttgcagtcg attcagagag cgttcgaact gggctttggc cccttcgtaa ctggtatcct
     2821 gcaaccagag tttcgtggtg acaaagagtt cgttacgggc gataccgctt tgtttcaggg
     2881 cgttcccgac ctggatttca ttttgataag aggcggcggt gtcaatcagt cgataaccgc
     2941 tgttaatagc gtcaatgacc gctctttcgc attcggcggc atcggtcatc tgaaatacac
     3001 caaagcccaa caggggcatt tcaataccgt tgttcagttt tactttttgc atggtgttat
     3061 ccttccgtta tcgttcatga gaagcataac gtaaagggaa gagctcgatt agacggcagg
     3121 atttgtcagg ggttatgaac aaaattcata aatctgtttg agggttgaga ttagtcgtgc
     3181 aagttcgata tccccggaat ttacagtccg atgaataaaa atgaatgtcc ataaaaaata
     3241 tattggttta tcccagcgta tatacttact tcgacgtact cccaatagca gttaattaaa
     3301 gacgggtcat tattattcac tctgaataat gaattattca ctgcccgcag agtaagaaat
     3361 ataacttaag tatctattta atgacttgca caaaaagcta aattttaccc ataaataaaa
     3421 atataatccc gcgcccaacc acctgatgag tggctacaga cactggatat attaggtggc
     3481 ggtgtacttt cttacataaa ggtatttcct tttctgtgga aaaggaaacc gggaaatccc
     3541 cggtttttct gacaagcaga cgccattatt tgtgtctgcc tatgttcgtt aattcgttca
     3601 tcaggaaatt atctcaatgt cacgttataa aacagacaat aaacagccac gatttcgtta
     3661 ttcagttctg gcccgctgcg tggcgtgggc aaatatctct gttcaggttc tttttccact
     3721 cgctgtcacc tttacgccag taatggcagc acgtgcgcag catgcggttc agccacggtt
     3781 gagcatggaa aatactacgg taactgctga taataacgtg gagaaaaatg tcgcgtcgct
     3841 tgccgctaat gccgggacat ttttaagcag tcagccagat agcgatgcga cacgtaactt
     3901 tattaccgga atggccaccg ctaaagctaa ccaggaaatt caggagtggc tcgggaaata
     3961 cggtactgcg cgcgtcaaac tgaatgtcga taaaaatttc tcgctgaagg actcttcgct
     4021 ggaaatgctt tatccgattt atgatacacc gacaaatatg ttgttcactc agggggcaat
     4081 acatcgtacc gacgatcgta ctcagtcaaa tattggtttt ggctggcgtc atttttcaga
     4141 aaatgactgg atggcggggg tgaatacttt tatcgatcat gatttatccc gtagtcatac
     4201 ccgcattggt gttggtgcgg aatactggcg tgattatttg aaactgagcg ccaatggtta
     4261 tatccgggct tctggctgga aaaaatcgcc ggatgttgag gattatcagg aacgcccggc
     4321 gaatggctgg gatattcgtg ctgagggcta tttacctgcc tggccgcagc ttggcgcaag
     4381 cctgatgtat gaacagtatt atggcgatga agtcgggctg tttggtaaag ataaacgcca
     4441 gaaagatcca catgcgatta ccgctgaagt gaattacacg ccagtgcctc ttctgaccct
     4501 gagtgccggg cataagcagg gcaagagtgg tgagaatgac actcgctttg gcctggaagt
     4561 taattatcgg attggcgaac ctctggaaaa acaactcgat acagacagca ttcgcgagcg
     4621 tcgaatgctg gcaggcagcc gctatgacct ggttgagcgt aataacaata tcgttcttga
     4681 gtatcgcaaa tctgaagtga tccgtattgc tctgcctgag cgtattgaag gcaagggcgg
     4741 ccagacggtt tccctggggc ttgtggtcag caaagcaact cacggtctga aaaatgtgca
     4801 atgggaagcg ccgtctttgc tggccgcagg cggaaaaatt acggggcagg gcaatcagtg
     4861 gcaagtgacg ctcccggctt atcaggcagg caaagacaat tattatgcga tttcagcgat
     4921 tgcctacgat aacaaaggca atgcctcgaa acgtgtgcag acagaagtgg ttattagcgg
     4981 agctggtatg agcgccgatc gtacggcgtt aacgcttgac ggtcagagcc gtattcaaat
     5041 gcttgctaac ggtaatgagc aaaagccgtt ggtgctgtct ctgcgcgacg ccgagggcca
     5101 gccagtcacg ggcatgaaag atcagatcaa gactgaacta accttcaaac cggctggaaa
     5161 tattgtgact cgtaccctga aggccactaa atcacaggca aagccaacac tgggtgagtt
     5221 caccgaaact gaagcagggg tgtatcagtc tgtctttact accggaacgc agtcaggtga
     5281 ggcaacgatt actgttagcg ttgatgacat gagcaaaact gtcactgcag aactgcgggc
     5341 cacgatgatg gatgtggcaa actccaccct gagcgctaac gagccgtcag gtgatgtggt
     5401 tgctgatggt cagcaagcct acacgctgac actgacagcg gtggactccg agggtaatcc
     5461 ggtgacggga gaagccagcc gcctgcgact tgttccgcaa gacactaatg gtgtaaccgt
     5521 tggtgccatt tcggaaataa aaccaggggt ttacagcgcc acggtttctt cgacccgtgc
     5581 cggaaacgtt gttgtgcgtg ccttcagcga gcagtatcag ctgggcacat tacaacaaac
     5641 gctgaagttt gttgccgggc cgcttgatgc agcacattcg tccatcacac tgaatcctga
     5701 taaaccggtg gttggcggta cagttacggc aatctggacg gcaaaagatg ctaatgacaa
     5761 ccctgtaact ggcctcaatc cggatgcacc gtcattatcg ggcgcagctg ctgctggttc
     5821 tacggcatca ggctggacgg ataatggcga cgggacctgg actgcgcaga tttctctcgg
     5881 cactacggcg ggtgaattag acgttatgcc gaagctcaat gggcaggacg cggcagcaaa
     5941 tgcggcaaaa gtaaccgtgg tggctgatgc attatcttca aaccagtcga aagtctctgt
     6001 cgcagaagat cacgtaaaag ccggtgaaag cacaaccgta acgctggtgg cgaaagatgc
     6061 gcatggcaac gctatcagtg gtctttcgtt gtcggcaagt ttgacgggga ccgcctctga
     6121 aggggcgacc gtttccagtt ggaccgaaaa aggtgacggt tcctatgttg ctacgttaac
     6181 tacaggcgga aagacgggcg agcttcgtgt catgccgctc ttcaacggcc agcctgcagc
     6241 caccgaagcc gcgcagctga ctgttattgc cggagagatg tcatcagcga actctacgct
     6301 tgttgcggac aataaaactc caacggttaa aacgacgacg gaactcacct tcaccgtgaa
     6361 ggatgcgtac gggaatccgg tcaccgggct gaagccagat gcaccagtgt ttagtggtgc
     6421 cgccagcacg gggagtgagc gtccttcagc aggaaactgg acagagaaag gtaatggggt
     6481 ctacgtgtcg accttaacgc tgggatctgc cgcgggtcag ttgtctgtga tgccgcgagt
     6541 gaacggccaa aatgccgttg ctcagccact ggtgctgaat gttgcaggtg acgcatctaa
     6601 ggctgagatt cgtgatatga cagtgaaggt taataaccaa ctggctaatg gacagtctgc
     6661 taaccagatc accctgaccg tcgtggacag ctatggtaac ccgttgcagg ggcaagaagt
     6721 tacgctgact ttaccgcagg gtgtgaccag caagacgggg aatacagtaa caaccaatgc
     6781 ggcagggaaa gtggacattg agcttatgtc aacggttgca ggggaacttg agatcgaggc
     6841 ctcggtgaaa aactctcaga agacggtcaa ggtgaaattc aaggcggatt tcagtaccgg
     6901 tcaggcgagc ctggaggtag acgccgctgc tcaaaaagtg gcaaacggca aagatgcctt
     6961 tacgctgacg gcaacggtta aggatcaata cggcaacctt cttcctggcg ctgtggtcgt
     7021 ctttaatctg cctcggggcg tcaaaccgct tgcagacggt aatatcatgg tgaacgccga
     7081 caaggagggt aaagcggaac tgaaagtggt ttccgtgact gccggaacct atgagatcac
     7141 ggcgtcagca ggaaatgacc agccttcgaa tgcgcagtct gtaacgtttg tggctgataa
     7201 gactacggcg accatctcca gtattgaggt gattggcaac cgtgcagtgg cggacggcaa
     7261 aaccaaacag acgtataaag ttacggtgac tgatgccaat aacaacctgc tgaaagatag
     7321 cgaagtgacg ctgactgcca gcccggaaaa tttagttctg actcccaatg ggacggcgac
     7381 aacgaatgag caagggcagg ctattttcac cgccacgacc actgtcgcag cgacatatac
     7441 actcacggcg aaagtggaac aggccgacgg tcaggaatcg acgaaaactg ccgaatctaa
     7501 attcgtcgcg gatgataaaa acgcggtgct cgctgcatct ccagagcgtg tagattctct
     7561 ggtggcggac gggaagacta ctgcaacact gacggttact ctgatgtcgg gtgtcaaccc
     7621 cgtaggagga accatgtggg tcgacattga ggctccggaa ggggtgacag aggcggatta
     7681 tcagttcctg ccgtcgaaaa atgaccattt cgcgagcggg aaaatcacgc gtacatttag
     7741 taccaacaag ccaggtacat acacattcac attcaactct ttgacatatg gagggtatga
     7801 aatgaaacca gtgactgtga caattaacgc cgttcctgca gatactgaag gcgctgagga
     7861 gaaataaaaa cgacgatgta ataaataata atggcgttga catgcgcagc gccattattt
     7921 actgaatacc taccctggca ccgattagac ggtggcaggg tagggtaaat cttatgtgat
     7981 ggagttacag gctaaaccgg gcgaacgggg ataaaaattt cgcaaacaat gttattgcta
     8041 tcaatttcga cgaagttttc attgtaataa aatcgctcaa tatcactgcc gttcactctg
     8101 gctaaccccc tggcaggtaa ttccatcaga tagaggtttc gcgcccattc agggtagtgc
     8161 tcccactcac cttcatagcg gaaagaggca tacagtcctc ggttaattgt ccactctttc
     8221 ggcatctctg aatcggtttg tttgcatacc gtaccggcaa aagtttcaac tgtgagcaaa
     8281 tcgaccgatt ttgttgatgg ttcgaacgtg gagacgaaat agatatccgt cttaaaattc
     8341 ttcatacatc tgactaaatg atgacgtcgc agtttgactt cttcatccgg taatttattc
     8401 gacacttcaa tatcatattt aaatgaattg ccaaaaatag gtgtctcagg aaagttgact
     8461 aatctacact ccgtcttttg ctgttgacgt attaaaaaag aagggaagat atttgcgaga
     8521 tcccaataat cacggtggcg gtattcacgg ggagagcagc cgaataactt tttaaattcg
     8581 cggctgaatg actgctgtga atcaaaatgc aaagagagtg caatatcaag catagatttc
     8641 gcggagagcc ggacaagaat ggcggcacga caaagccttc gtttgcgaat gtattctccc
     8701 aaaggcacat gcatgaaatt tcggaacaga agctggatgt tgcgtctgct gtagccagat
     8761 ttctgtgcga tatcttcgat tgaaataggg tgctcaagat tgcactcaat ccactccagg
     8821 agctgctgta gaatcttctg cctgatcatc acatactccg ttaattcttt gtcatatcgc
     8881 acattttcgt tatgtcctgg gccctaagtg tatcaaaaac agcaagtccc aaggcaaaag
     8941 cactgatagc gtctgcttca ttgccccaaa aatagagagg tttcagtcct acattattaa
     9001 tgaatttttt gcataagtga tatcaaaatc cacatactaa tttgaggtta cgttttaacg
     9061 tagactcatt gttcatacct aatggaggga ctgacagtgg aattttccgt attaagtaac
     9121 aatctgaaaa tgccgatgat gggatttggt gtttttcagg ttaccgataa agacgtgtgc
     9181 aaacagtcag tgttgaatgc tatccgcacg ggttatcgac tcatcgacac tgcagcggta
     9241 tacggtaatg aagatgccgt cggtgaagcc gttcgtgaag ccatatctga aggtttatgt
     9301 acccgagaag agttatttat aacgtctaaa ttgtgggtgc aggatatgtt gaatcaggat
     9361 attgcagcag caggtattga agcatcatta aaaaaatcgg ggctagaata cttcgatctc
     9421 tatttattgc accaggccat gcgcgattat tttagtgcgt ggcgtgcact cgaagatgcc
     9481 tatgaagagg gcaaattaaa agcgattggg gtttccaatt tctaccctca tgttctggcg
     9541 aacttttgtg aaacggtaag agttaaaccg atggtcaacc aggtcgagtt gcatccctat
     9601 tttgcccaac cagaggcgct ggcaaccatg aagtattata acgtgcagcc tgaagcatgg
     9661 gctccgttag gtggtggacg acataaaccc tttgaaaata atctgcttca gagtattgca
     9721 gatgcccatc aaaaatcgat ttctcaggtc attctgcgtt ggaatattca acggggagtg
     9781 gtcgttattc cgaaatcgac acatcaacag cgtatcgaag aaaattttgc tatttgggat
     9841 ttctcactga cagagaaaga aatggcgcaa attagttcgc ttgatttggg ttatgttggg
     9901 gaatcggtaa aacattttaa tcctgagttt gttcgtggtt gtcttgctgt aaaaatacat
     9961 gattgatatt aatcacttat tttacctgag acgacaagaa tcttttaata ggggagtgat
    10021 attgaccttc actctgtcat atctccggta atatggcgtc aggcttcatc atcagaaagg
    10081 gagcctgacg cctgaaaaag tgaacaacag acagtgttcg gattatcaat attcagtttt
    10141 taacgttgaa ctggattcat tactgcgttg tttaagaata tcccgcgccg aatctgccat
    10201 tatcatgaca gcacctgcca gcatcagagt atctttcaat accagacgac cagcaccgga
    10261 taaataaggg aaaccatgat gagcgtcacc caatgcgggt acccatgcct ccggggtggt
    10321 gattaaaaat gagagtgtta ccagcggcgt ggtaaatgcc atcagcccac ccaataaacc
    10381 taaccagcga ttgacaggat tagccaaaac cagcagagca ataatcacct ccacgacgcc
    10441 aagaccgttg gaaaaaccat aggtattatt agccgtttgc catgctcttg cttctggttt
    10501 gtattcgcct tcgtgagtca gatgctgttt atagtcttcc gggtgttcat aaaagaacga
    10561 cattagtgga ctgtttgcga cgaatggtgt aatgctgtct gcctcgtaag ggacaaactt
    10621 taataaccca atccacataa aaacaatcgc aatactcaga cgaatcaatg tcaggccaat
    10681 tttatctccc cgacttaaca ggtgcaggta tctttccatt gtaaacatcc ttatttatac
    10741 aatactgctg ttgcggttga attattatca attcgcatcg atgtaacttt ccaggatgaa
    10801 gcattatatt ttctggccat atcagcaatg gcattctcaa gaactgtcgg tgatgctgat
    10861 actgatgaga cgctgatatg tttcagaatt attttatcat ctgcattggt gga
//
LOCUS       DAZFJL010000153        10862 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFJL010000153 DAZFJL010000000
VERSION     DAZFJL010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.155"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..297)
                     /gene="chbB"
                     /locus_tag="ACHJ8C_004002"
     CDS             complement(<1..297)
                     /gene="chbB"
                     /locus_tag="ACHJ8C_004002"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO5553781.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAA"
     gene            complement(597..935)
                     /gene="osmE"
                     /locus_tag="ACHJ8C_004003"
     CDS             complement(597..935)
                     /gene="osmE"
                     /locus_tag="ACHJ8C_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO5553782.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQATK"
     gene            1137..1964
                     /gene="nadE"
                     /locus_tag="ACHJ8C_004004"
     CDS             1137..1964
                     /gene="nadE"
                     /locus_tag="ACHJ8C_004004"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO5553783.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2194..3081
                     /gene="cho"
                     /locus_tag="ACHJ8C_004005"
     CDS             2194..3081
                     /gene="cho"
                     /locus_tag="ACHJ8C_004005"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO5553784.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLSEKRVDVVYAKEVDFSRAPNLFGLFATRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHDDHALRLRQSLERLRVVCW
                     PWKGAVALKEQHPEMTQYHIIQNWLWLGAVNSLKEATTLIRAPAGFDHDGYKILCKPL
                     LSGNYEITELDPVNDQQAS"
     gene            complement(3041..3616)
                     /gene="ves"
                     /locus_tag="ACHJ8C_004006"
     CDS             complement(3041..3616)
                     /gene="ves"
                     /locus_tag="ACHJ8C_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO5553785.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASITSIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFSFAADLVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3819..4304)
                     /gene="spy"
                     /locus_tag="ACHJ8C_004007"
     CDS             complement(3819..4304)
                     /gene="spy"
                     /locus_tag="ACHJ8C_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO5553786.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHEDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4634..5602)
                     /gene="astE"
                     /locus_tag="ACHJ8C_004008"
     CDS             complement(4634..5602)
                     /gene="astE"
                     /locus_tag="ACHJ8C_004008"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO5553787.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMANDTLNFM
                     PFEKGTLLAQDEEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5595..6938)
                     /gene="astB"
                     /locus_tag="ACHJ8C_004009"
     CDS             complement(5595..6938)
                     /gene="astB"
                     /locus_tag="ACHJ8C_004009"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO5553788.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKKLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEKKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVSGFMAIEVPTAQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWCY
                     LNELLAADNPISSLKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDTLTQLLDLGSVYPFQREGGGNG"
     gene            complement(6935..8413)
                     /gene="astD"
                     /locus_tag="ACHJ8C_004010"
     CDS             complement(6935..8413)
                     /gene="astD"
                     /locus_tag="ACHJ8C_004010"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO5553789.1"
                     /translation="MTLWINGDWVTGQGALRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFGDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGDAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGTQGDAFLARLVAVSQRLTPGTWDDEPQPFIGGLISEQAAQQVVTAW
                     QELEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVTGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRLGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8410..9444)
                     /gene="astA"
                     /locus_tag="ACHJ8C_004011"
     CDS             complement(8410..9444)
                     /gene="astA"
                     /locus_tag="ACHJ8C_004011"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO5553790.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSVRIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPEWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9441..10661)
                     /gene="astC"
                     /locus_tag="ACHJ8C_004012"
     CDS             complement(9441..10661)
                     /gene="astC"
                     /locus_tag="ACHJ8C_004012"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO5553791.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLHGLRELCDRHNA
                     LLIFDEVQTGVGRTGEMYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVS
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNSINHHYSLFSEVRG
                     LGLLIGCVLNADYSGQAKQLSQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
BASE COUNT         2641 a         2975 c         2675 g         2571 t
ORIGIN      
        1 cgctgcaacc gcagccttaa gcacgcctaa accatcgact ttgccataaa gcagcgagtc
       61 aattacttca accggtttgt tgggtaacaa acgctggatt tcgggcaaca tataagcaat
      121 ctgcggccct aataacacga catcggcatt ctgacctttt tcaccagcca gtgtttccgg
      181 aaatgcttca ataatgaccg gaacttcata tttttctgcc tgtgcgcgca tttttgatac
      241 cagtaaagag gtagacatgc ccgcagaaca aaacagataa atgtgtttct tttccataaa
      301 actgccctca tcgacgatta tctgtcagcc agacactccg caagccttaa cctgcttcca
      361 tgctctgggt aacttgcgaa accaacatga ctttttttgt ggctgaaagg agtatacggt
      421 ataggcctga gcaatagtat ttctttgacc ttagaaattg tctctccttg acctgtggtt
      481 atgaccctct tcacatttcg ggcaaataat tcgcgaagat aattaagaac acaggccaaa
      541 aaaaaaccgg cgcaatggcc ggtttccgtt gttactcaag ctttcaggcg aagtgattac
      601 ttcgtagcct gtggatcagt gtcgtattca gcacaggtct gataaccgga gttgatgaca
      661 tgtccggtat catctaacgc gacaaagtag gtttctgctt taccatcacg ttgacccagg
      721 atgtaggtct ggcaagtacc acgagcatgg atcatgctca cttcagacga aggtttaccc
      781 gcaatttgtg caacctgcgc ccggctcatg ccttttttga cgtctttcac cacaggctgt
      841 acaaactggt ctttggtacg atcataagcc gtacaacccg ccagcatggt taataccgcc
      901 gctgcactca gaattcctgc catattcttg ttcatattcc gtcctcttgt ttatcagcgt
      961 gttagataag cctggaatac attgggcgct ttttcaagcc cgtgaacgaa acggctccgc
     1021 tttcagagga ttcctgtatg acgttttaac caccattcag cccgctgtcg cttgtcgttt
     1081 cagtagcaac gggttagctt taaggaagtt ttgtcttttc tgtctggagg ggttcaatga
     1141 cattgcaaca acaaataata aaggcgctgg gcgcaaaacc gcagattaat gctgaagagg
     1201 aaattcgtcg tagtatcgat tttctgaaaa gctacctgca aacttatccg ttcattaaat
     1261 cactggtgct cgggatcagc ggcggtcagg actccacgct tgccggtaag ctgtgccaga
     1321 tggcgattaa tgagctgcgc caggaaaccg gcaacgaatc actgcaattt attgccgtac
     1381 gcctgcccta tggtgttcag gccgacgaac aagactgcca ggatgccatc gcctttattc
     1441 aaccggatcg cgtattaacc gttaatatca agggcgcggt actcgccagc gagcaggcat
     1501 tgcgggaagc aggcattgaa ctgagcgatt ttgtccgtgg caatgaaaaa gcgcgtgagc
     1561 ggatgaaagc acaatatagc attgcgggta tgaccagcgg tgtcgtggtg ggcaccgatc
     1621 atgcagcaga agctattacc ggattcttca ctaaatatgg tgacggcggt acggacatta
     1681 atccgctgta tcgtctcaac aaacgtcagg gtaaacagtt actggcggca ttaggttgcc
     1741 cggaacacct ttataagaaa gcgccaacgg ccgatcttga agatgatcgc ccttcactgc
     1801 cagatgaagt ggcactcggc gtgacctatg acaatatcga cgactatctg gaagggaaaa
     1861 acgtgcctga acaggtcgcc agaacaatag agaactggta tctgaaaacc gaacataaac
     1921 gccgtccgcc aattaccgtt ttcgatgatt tctggaagaa gtaataattt gcacatattg
     1981 gtttgtgcga aaaagagtac tttgttcacg ccggatgcgg cgtgaacgcc ttattcgccc
     2041 cacaaaacta tgcaaattca atatattgca taagtcgaat aggcctgaca ggcgtagcac
     2101 gtcagacggt gtaacctttg tcatcaaccc gcctcttttt taatcgctcc ccgcctgtta
     2161 cactggatag ataaccagca ttcggagtca acagtggtaa ggcgtttaac ttctccgcgg
     2221 ctcgaatttg aagctgcggc aatttatgaa tatcccgaac atttacgttc attccttaat
     2281 gacttaccca cccgaccagg ggtgtatctg tttcatggcg aaagtgacac catgccgctc
     2341 tatatcggca aaagcattaa catccgcagt cgggtgcttt ctcatttacg cacccctgat
     2401 gaagccgcca tgctacggca atcccgacgc atcagctgga tatgtaccgc cggtgaaatc
     2461 ggcgctctgc tccttgaagc gcgattaatc aaagaacaac agccactgtt taataaacgc
     2521 ttgcggcgca atcgccaact ctgtgccctg caattaagtg aaaagcgcgt cgatgtggtg
     2581 tatgccaaag aggtggattt ttcacgagcc cccaacctgt ttggcctgtt tgccaccagg
     2641 cgcgcagctt tgcaagcatt gcagaccatc gctgatgaac aaaaactttg ttatggcctg
     2701 ttgggactgg agccgttaag tcgcggtcgt gcatgttttc gttcagcgct aaaacgttgc
     2761 gccggagcat gctgcggtaa agagagccat gacgatcatg cgctacgttt gcgccagtct
     2821 ctggaacgtt tacgagtcgt ttgttggccg tggaaaggtg cggtggcgct gaaagaacag
     2881 cacccggaaa tgactcaata tcatattatt caaaactggc tgtggctggg ggcggttaat
     2941 tcgctgaaag aagcgacaac gttaattcgg gcacccgccg ggtttgatca cgacggttat
     3001 aaaattcttt gtaagccgct gctttccggt aactatgaaa ttactgaact tgatccggtg
     3061 aatgaccagc aagccagttg atttcactga acaacagttt accttgcggt tgcaaaagcc
     3121 gcagggtgtg cctgccatcg aaccagcacg cgccctggtc ggtagtgagc agtttgtcgc
     3181 ccaattgcca cgccccgttt attacaaaca ccaccccgcc gcgtgaacca aaggtagtaa
     3241 acgtacgttc ggcaattctg actttcgcct tacagacatc caggcgtgtc atgatgttga
     3301 aatccatcga catctgccct gccgtcagtt tcgcttttac caccaggtcc gctgcaaagg
     3361 aaaaaggttg taacggtttt aaggtatggt taaagcggtc tgcgctttca aggaacatct
     3421 cgccgccttc cagcaacgtc actatccttt ccataccggg aaacaaagaa aactccccat
     3481 tcgccgcgat ggatgtaatg ctggcacgcc agtaaaaatc acgttttgcc ggtgggaaag
     3541 tgcaaatttc gcgcgtttca ccggcagcgt ttcgccacag attcaccgac attttacgca
     3601 tatcaaagta ttccataccc gctcccggcc cgttcttttc attgttatcg ttcaggtaac
     3661 cgtggatggt tatcggcaac tcgggtgatt tacgttagtg gcgatcaggc tttttttgca
     3721 tctgtttgag aagagaaaag aaaaccgccg atcctgtcca ccgcattact gcaaggtagt
     3781 ggacaagacc ggcggtctta cgttttttgg ctgaaagatt attcagcagt tgcaggcatt
     3841 ttaccttttg ccgctggacg ttctgtcaga cgcttctcaa aattagcatt aaattgcttt
     3901 ttctgttccg gcgtcaggat gttgtaaatc ttgttctggg tttccatgtg cgccagcatg
     3961 ttagctttgc gctgttcttc catttttgcg atctgcgctt cagcttttgc tttatcgaag
     4021 gtatcgctgg caatgatgtc atgcattgcg cggcgttctt ccagcggcgg acgtttcatc
     4081 tggtcacgct ggcctttcat gatttcgcgg atctgctgtt tctgcgcgtc ggtcaggttc
     4141 aggtctttga acatcatgtc ctcatgcgga ccgaacttgc ctttatggtg catcatcggc
     4201 ttcgcgtcag ccggtgctgc ggtagtggtg tctgcggcat gggccaggtt agccgcgcca
     4261 agagccaggg tagaggcaac aaacagtgca gttaatttac gcatattcta tatccttcct
     4321 ttcagttatt tattacggct ttcttaagta gcgtgccgtg ttgacgagat taactttact
     4381 gactttagcg tcaattaatc agagcaacgg taaaacaatg aaagtgtaaa aaacactttt
     4441 gcgccaatta tggagaaaaa aagaaaattt gatggagagt gatgaaagaa tattacaaca
     4501 cgatgatttt gcagagatta tgaagaacta taccggatga ctggtgataa ataaagcaaa
     4561 taaccaggat taatctgtat taatatataa gaaagcaact taatacccgc agaatgattt
     4621 ctgcgggtaa gtattagctt attttttcga gcattaatcc cgcacgtaat cccaacgcta
     4681 ccaacggatt agggaataac acatactcta catcatgggt tacggtaaaa cgttcctcct
     4741 cgtcctgcgc cagcaatgtt cctttctcaa acggcataaa attcagcgtg tcatttgcca
     4801 tatgcatttc gaaggacggc gagtgacgag taatttgcga aaccacccga taacggagcg
     4861 gcggtgttct cacgataccg acactctcac cagatagcag cgcagcaatt gcgctggcag
     4921 ttactgcaaa ctggcgaaga tcgttttgcc caaagggcaa cgctttgcca agttccagcg
     4981 tacaggccag cgcgccaaaa tgtctggcgc tgaaatgggt aaacgtacca ccaggctcct
     5041 gatggaacac cagcgcctcc agccccgccg cacccagcca cgtcagaaat ttctcgtccc
     5101 agggaatgtc gcgttgcggt aatacaccga actgcggatg caaggagcca cgaattgcgg
     5161 tatgtagatc aaggtgccag cgcacagatt ctttgccctg gtcataaaaa tcttccaggc
     5221 actgctccag ttcgcgcgcc cgacaggttt ccccgctttc agcaaacagt tgccaacgac
     5281 cgccaaacat tcgattcata tcgctatggc aataacgttt cccttgcttc agcgcaggag
     5341 gattcccgag gatcaccagt aaccgccaac gtaacgggat ctcgccgtga gatatcgcgc
     5401 caagcaacgc gtccagcatt tccacaggtg ccgtctcatt accgtggatc cccgctgaaa
     5461 tcaccagcgc gccttgcggt ggcgttaatg gcgtcagttc cagcacacca tcgcccagcc
     5521 agcgccagcg aacgccgttg atttctcgct cggtgataac cggtttttta cccgttaagg
     5581 ttagagcaag aaaattatcc attgccccct ccctcgcgct ggaatggata aaccgaacca
     5641 agatccaata attgagtcag tgtatccagc gcttcccttc cttcgcgcag caattgtgga
     5701 tcggcaagat ccgccgcagt aagacgatcg cggtagtaac gatccaccca gtcattgagc
     5761 gcattaaaca gcgtatcgtt catcatcacc gccggattca ccgcccggcg ttcttcttct
     5821 gtcaacacca cgcgcaaccg caggcacgcc gggccaccgc cattcgccat gctttcacgt
     5881 aaatcaaaaa cttttagtga gcttatcggg ttatcggcag caagcaattc attgagataa
     5941 caccatactc cagcatgttc ccgacattcc tgaggcagca ccaacatcat ggaaccatca
     6001 tcgcggctca gcagttggct gttaaacaga taagtagaca ccgcatctga cacggaaacc
     6061 tgcgccgtgg gcacctctat tgccataaaa ccactgaccc gtgcacgcag atttgccagt
     6121 aactgtgact ggcgagcgaa cgcctgttga tggcaaaaca gcacctggcg gttactcacg
     6181 gcaatcacgt cattatgaaa aacgccctgg tcgataacgt ccgggttttg ctgggcgaaa
     6241 atcacctgtt ggggattcac ctgattcaac cttgccaccg cctcgctggc ttcacgggtc
     6301 tgccgcgccg gataacggga aggccgggta tcattgccct cttctcgccc gtagacaaaa
     6361 agttgcatac ccggttcacc gtaatgaccg ccgagacgat tgtggtttgc cgccccctca
     6421 tcaccgagca aggctacctg cggcaacgcc gaatggacgc taaatttctt ttcgtcgtta
     6481 aaaatcgctt ttaacagcga ttcggtgacg ggggcttcca gcgaacggtg aaatttattg
     6541 ttcaggttag caacagtgag atgcactttg ccatccagcg tatcggcgga tggcgcgatc
     6601 gttgccgcat tggctaccca cattggcgaa gcggaactga cgctggaaag ccagtgcggt
     6661 gcctggcgtg caactttttc cagtacctgc tcatcgctac cactgaatcc caactgacgc
     6721 agcaccggaa taaacggacg ctcgtgcggc gggatcacgg cctgggggaa tcccgcatcg
     6781 gcaagttttt tcattttcag taagccctgt tttgccgcca gtcgcgggtt agacacctga
     6841 aaacggtgac gggtagaggc ttcattacca aacgacaggc ccgcgtaatg gtgcgtcagc
     6901 cctaccagcc cgtcaaaatt gacttcccag gcgttcatcg caccacctca tcggaaaaat
     6961 ccaacccggg gttaagcgtt gcgggcaacg ttaacgagtc cgactctagg ctcgccatcg
     7021 gccaggcaca ataatctgcg gcataccagg cgctggggcg atgattgccg gaagcgccaa
     7081 tgccgccgaa tggtgcggta ctggcagcac cggtaagcgg tttgttccag ttaacaatcc
     7141 ccgcccgtgc ctccagcaac agttgatcga atttttcccg ctcgggggaa accagaccgc
     7201 aagagaggcc gaggcgagtg ttattcgcca ttcgaatcgc ttcatcgaaa ttgtcataac
     7261 gccagacgcg caatagcggc ccaaacactt cttcatccgg cacgccagta acgcctgtca
     7321 tttcaatgat ccccggcgtc agcaacgatg tccctgcttg taataagcgc ggcgcaagca
     7381 gggtccgtcc gcccatcgcc tccagttcct gccaggcagt aaccacctgc tgtgcggcct
     7441 gctcagaaat cagcccacca ataaatggct gcggttcgtc atcccaagtg cccggcgtta
     7501 accgctggct gacggcaacc aggcgagcaa gaaatgcatc gccctgcgtt ccacttttca
     7561 gcaataaacg gcgggcgcag gtgcagcgtt gcccggcagt gacaaacgcc gactgaatgg
     7621 tcagatggac agcggcatcg atatccgcca cctcatcgat aattagcggg ttattaccgc
     7681 ccatctcaag ggcgagaatt ttctccggct gaccggaaag ctggcgatgc agctggtagc
     7741 cggtattggc gctaccggta aacagcaagc cgtcgagatc ctccagcgca ctcagcgcct
     7801 gacccgtttc acgcccgccc tgcaccaggt tcagcacgcc cggcggcaag ccagcctgct
     7861 gccataaacg cattaccgcg tcgccactcc acggtgtcag ttcgctgggt ttaaagataa
     7921 tggtgttacc tgccagcaat gccggaacga tatgtccgtt cggcaaatga ccagggaaat
     7981 tatacggccc aaacaccgcc agcacgccgt gcgggcgatg tcgcaggctc gctgctccgt
     8041 ccggcatttc gctacgctgc tcaccggtac gaacgtgata cgccttaatt gatatcgcga
     8101 ttttattgat catcgccgtc acttcggttg ccgcttccca gcgcggcttg ccggtttctc
     8161 tggcaataat cgcggttaat tcggctttat tgctttccag caatccagca aagcgttcaa
     8221 ctcttacctg acgatcgcca aatgagagcc gagcccagcg cggaaacgcc gcacgggcag
     8281 cccgacaagc ctgcccgacc tgagcggcat cggcatcatt gccttgccat aacacctcgc
     8341 ccgataccgg attacgcttc acacgcaatg cgccctggcc cgttacccag tcaccgttga
     8401 tccataaagt catgctgttt tctcctctgc gcacaggcgc accagacgaa cgcgatcccc
     8461 tgcgtggcat ttgagggcat ccagttgtgc ggcggttaaa atcaaacgct cagtcgctgg
     8521 atcggtacgc accagcacca cgcggaaatg gtgataattt tcattggcga ccaggcaggc
     8581 cggaaaatcg ccctgcgcag gctgcccttc ggccacttcc accagccgac ttttacggat
     8641 ggcgcgcacg cggtcgatat cacattcaag tgtcggcccg ccgtcaaaga tgtcgatata
     8701 gttacggtag cgaaaacctt ctttctccag caccgcacgg gcaggcgcgg tttgcggatg
     8761 tacctgaccg atgacgtcct gggcttcctg ggataaaaag tgggtataga tcggatgttt
     8821 cggcatcagt tctgcaataa atgccttttg cccggtgccg cagagaaaat cagcgcggct
     8881 aaaatccatc gaaaagaagc gtttaccgag gctttgccag aacggtgaat agccgtgttc
     8941 gtcaatcacc ccgcgcattt cggcgaccac tttgtcatta aacttgtcgc gaaaagccgc
     9001 cataaacata aagcgcgatt tcgacagcaa atagccgttg ccctctttgc gccactccgg
     9061 gtcgagaaac agcgtgcaca gctcgctgct gccggtgtga tcgttactga gaaacagcgt
     9121 cggcaatgcg ttatagacat tcagctcttt cgaagcgtga accagcgtgc cgacacgata
     9181 gttgtaccag ggatcgttca gcccaaccgc cacctcaatg gcgcaaatcc ccgccacagt
     9241 gcctgtctca ctatcttcca gtacaaacac ataaccctgc tcacttttgg gcagttcgcc
     9301 ttgccaggtt ttgattgccc tttcaatacg caccgaaagc gtggcttcat tggcgggaag
     9361 cgacgtcagg ccgccgcccg ttttgctggc aagctgcatc agcgccgaga catctgagcg
     9421 ctcaacggga cgaatgacca tcatgatgaa cctcggctaa caaagtgttc gcaagcagct
     9481 gcaaagcgat ccagtccggt cgtcacctct tcttcgctga cattgagcgc tggcgcaaaa
     9541 cgcaccacgt tgccacccgc aatcagcacc atcacgcctg ctttcgccgc ttcctgagag
     9601 agctgtttcg cttgcccgga gtaatcggca ttcagtacac agccaatcaa aagacctaag
     9661 ccgcgaactt cactgaacaa actgtagtgg tgattaatgg aatttagacg ctcaacaaac
     9721 cagtcgtgac gctgtttaac cccgttaagc atctctggcg tgttgatgag atccagcact
     9781 ttgcctgcca ccgccgaggc cagcggattg ccgccatagg tggtgccgtg agtgctaacg
     9841 gtcatcacgc tggcgcactc ttcggttgcc aacaacgcac caaccgggaa accgccgccc
     9901 agcgctttgg cggtagttaa cagatcaggc gtcacgccgt agtgcatata ggcatacatt
     9961 tccccggtgc gcccgacacc ggtttgtact tcatcaaaga taagcaacgc attgtggcgg
    10021 tcacacaatt cacgcagacc atgtaaaaac gcgttgctgg ctggcaccac accgccttcg
    10081 ccctggatgg gttcgacaat caccgcacag gtggcgtcgt caatcagcgc gctggcagag
    10141 ttaatatcgt tatatgcggc atgacgaata tccggcggca gtggcgcaaa atcctgtgaa
    10201 taggctggct gaccgcccgc actgacagta aacagcgtgc gaccatgaaa cgcatttttg
    10261 aacgccacga tgccgctctt atggctgccg tagcggtcgt gagcgaattt acgcgccagt
    10321 tttagggccg cttcgttggc ttccgcaccg gagttacaaa agaagacgcg atcggcaaac
    10381 gtggcgtcga tcaatttttt cgccagtcgc agtaccggct cgttggtgta accgttgccg
    10441 gtatgccaga acttactcgc ctgttcgttc agcgcctcac gcagttccgg atgcgcatgg
    10501 cccagcgcgt tcaccgcaat gccacccgcg aagtcgatat actctttccc ctgctgatcc
    10561 cacaagcgcg aaccttcgcc acgtaccggt ataaacggtg ccggggcgta aacaggtatc
    10621 atccattcat caaagttttc acgcgtaatt ggctgagaca tagcgacctc tacagtaaat
    10681 agttcgttat ttatatgtta ataataagta atgtttgcgc tgtaaatgta gattgcaggg
    10741 ttcgtgccag ccagtgataa aagtgcataa acggcggagg ctaactggaa atcaaggagt
    10801 tataaccaaa ccatatgcat ttaaagtgca tataaagtga atacgtttgc gatgtgggtg
    10861 aa
//
LOCUS       DAZFJL010000154        10858 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFJL010000154 DAZFJL010000000
VERSION     DAZFJL010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.156"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            39..653
                     /gene="evgA"
                     /locus_tag="ACHJ8C_004013"
     CDS             39..653
                     /gene="evgA"
                     /locus_tag="ACHJ8C_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO5553792.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            658..4251
                     /gene="evgS"
                     /locus_tag="ACHJ8C_004014"
     CDS             658..4251
                     /gene="evgS"
                     /locus_tag="ACHJ8C_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO5553793.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADGDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRIRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEDGEVDIVLSHLVASPPLNDDIAATNPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPYEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFLLTRKDSIVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDETGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPAAIYSEDRENNVSFAEIFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVQNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTLPERYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     YIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTIFTITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAYIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEITVFCQQND"
     gene            complement(4307..5452)
                     /gene="yfdE"
                     /locus_tag="ACHJ8C_004015"
     CDS             complement(4307..5452)
                     /gene="yfdE"
                     /locus_tag="ACHJ8C_004015"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO5553794.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVHNQAILKEYIERTLKTQVAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5526..6470)
                     /gene="yfdV"
                     /locus_tag="ACHJ8C_004016"
     CDS             complement(5526..6470)
                     /gene="yfdV"
                     /locus_tag="ACHJ8C_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO5553795.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6540..8234)
                     /gene="oxc"
                     /locus_tag="ACHJ8C_004017"
     CDS             complement(6540..8234)
                     /gene="oxc"
                     /locus_tag="ACHJ8C_004017"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO5553796.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQSDEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8288..9538)
                     /gene="frc"
                     /locus_tag="ACHJ8C_004018"
     CDS             complement(8288..9538)
                     /gene="frc"
                     /locus_tag="ACHJ8C_004018"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO5553797.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKSIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKSAPLLGEH
                     TAAVLLELGYSDDEIAAMKQNHAI"
     gene            complement(10050..10682)
                     /locus_tag="ACHJ8C_004019"
     CDS             complement(10050..10682)
                     /locus_tag="ACHJ8C_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO5553798.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVKQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         3187 a         2449 c         2227 g         2995 t
ORIGIN      
        1 gggagaaggg aaatgcttca ttgcaaaggg aataatctat gaacgcaata attattgatg
       61 accatcctct tgctatcgca gcaattcgta atttattgat caaaaacgat attgaaatct
      121 tagcagagtt gactgaaggc ggaagcgccg ttcagcgggt ggaaacactt aagcctgata
      181 tcgtcatcat tgatgtcgat atccccggag ttaacggtat ccaggtgtta gaaacgctga
      241 gaaagcgcca atatagcgga attattatta ttgtctccgc taaaaatgac catttttacg
      301 ggaaacattg tgctgatgct ggcgctaatg gtttcgtgag taaaaaagaa ggcatgaaca
      361 atatcattgc agctattgaa gctgcaaaaa atggctactg ctatttcccc ttctctctca
      421 accggtttgt tggaagttta acgtccgacc agcaaaaact cgactcctta tcgaaacaag
      481 aaattagtgt catgcggtat attcttgatg gcaaggataa taatgacatt gctgaaaaaa
      541 tgttcatcag caacaaaact gtcagcactt ataaaagtcg cttgatggaa aaattagaat
      601 gcaaatcact gatggatctt tacacattcg cacagcgtaa caaaatcggc taaccacatg
      661 aagtttttac cctatatttt tcttctctgt tgtggtcttt ggtcgaccat aagtttcgca
      721 gacggagatt acatcgaata tcgtggcatc agtagtaaca accgtgtcac acttgatcca
      781 ctacgtctga gcaacaagga attacgttgg ctcgcgagca aaaaaaatct tgtgattgca
      841 gtacacaagt cccaaacagc tacgttgttg cataccgatt cgcagcaacg gattcgtggt
      901 attaatgctg attatttaaa tcttttaaaa agagcgttaa atatcaaatt aacactccgg
      961 gaatacgcag atcatcaaaa agcaatggac gcgctggaag acggtgaagt tgatatagtg
     1021 ttatcacatt tagttgcttc gccgcctctt aatgatgaca ttgctgcaac taatcctctg
     1081 ataattacct ttccggcgct ggtcactacc cttcacgatt caatgcgacc gcttacctca
     1141 tcaaaaccag taaatattgc tcgagtagca aattatcccc catacgaggt aattcatcaa
     1201 tcatttccaa aagcaacaat tatctctttt acaaatttat atcaggcatt agcatccgtc
     1261 tcagccggac agaatgatta ctttattggt agtaacatca ttaccagcag tatgatttcc
     1321 cgttatttta ctcactcctt aaatgtagtg aagtattata actcaccgcg tcaatataac
     1381 tttttattaa ccagaaaaga ttcaatcgtt cttaatgaag tactcaatcg atttgttgat
     1441 gctttaacaa atgaagttcg ctatgaagta tcacaaaatt ggcttgatac aggaaacctg
     1501 gcctttctga acaaaccatt agaactcact gaacatgaaa aacagtggat taagcagcat
     1561 cccgatttaa aggtgctgga aaatccttac tcgccaccct attctatgac agatgaaact
     1621 ggctcagttc gaggcgtgat gggtgatatt cttaatatta ttaccttgca aacaggttta
     1681 aatttctctc cgatcaccgt ttcacacaat attcatgctg ggacacagct taaccccggt
     1741 ggatgggata tattgcccgc cgctatttat agtgaagatc gagaaaataa tgtttcattt
     1801 gctgaaatct tcataacaac gccttacgtt tttgtcatgc aaaaagcgcc tgacagtgaa
     1861 caaacattaa aaaaaggaat gaaagttgcc attccatatt attatgagct tcattcgcaa
     1921 ttaaaagaga tgtatccgga ggtagagtgg ataaaagtcg ataacgccag cgctgcattt
     1981 cacaaggtca aggaaggtga acttgatgct ctggtcgcga cacagttaaa ttcgcgttac
     2041 atgatcgacc attactatcc taatgaactt tatcattttc ttattcccgg cgttcagaat
     2101 gcatcacttt cgtttgcttt tcctcgcgga gaaccggaac ttaaggatat tattaataaa
     2161 gcactgaatg caattccccc aagcgaagtt ctgcgcctga ccgaaaaatg gattaaaatg
     2221 cccaatgtga ccattgacac atgggacctc tatagcgagc aattttatat tgttacgaca
     2281 ttatccgttt tattagttgg cagtagcctt ttatggggat tctacctgtt acgctcagtt
     2341 cgtcgtcgta aagttattca gggtgattta gaaaaccaaa tatcattccg gaaagcgctc
     2401 tcggattcct taccgaatcc aacttatgtt gtaaactggc aaggtaatgt cattagtcac
     2461 aatagtgcat ttgaacatta tttcactgct gattactata aaaatgcaat gttaccatta
     2521 gaaaacagtg aatccccctt taaagatgtt ttttctaata cgcatgaagt cacagcagaa
     2581 acgaaagaaa accgaacaat atacacacag gtttttgaaa ttgataatgg catcgagaaa
     2641 agatgcatta atcactggca taccttatgt aatctgccag caagcgaaca tgctgtttat
     2701 atttgtggtt ggcaagatat taccgagacg cgtgatttaa ttcatgcact cgaagtagag
     2761 agaaataaag cgatcaatgc aactgtcgca aaaagccagt ttctggcaac aatgagtcac
     2821 gaaataagaa caccaataag ctccattatg ggcttcctgg aacttctgtc gggttctggt
     2881 cttagcaagg aacaacgggt ggaggcgatt tcacttgcct acgccaccgg acaatcactc
     2941 ctcggcttaa ttggtgaaat ccttgatgtc gacaaaattg aatcgggtaa ctatcaactt
     3001 caaccacaat gggtcgatat ccctacttta gtccagaaca cttgtcactc tttcggtgca
     3061 attgctgcaa gcaaatcgat cgcattaagt tgcagcagta cgcttcctga acgctacctg
     3121 gttaagatcg accctcaggc gtttaagcag gtcttatcta atttactgag taatgctctc
     3181 aaatttacta ccgagggagc agtaaaaatt acgacctccc tgggttacat tgatgacaac
     3241 cacgctgtta tcaaaatgac gattatggat tctggaagtg gattatctca ggaagaacaa
     3301 caacaactgt ttaaacgcta tagccaaaca agtgcaggtc gtcagcaaac aggttctggt
     3361 ttaggcttaa tgatctgcaa agaattaatt aaaaacatgc agggcgattt gtcattagaa
     3421 agtcatccag gcataggaac gatatttacg atcacaatcc cggtagaaat tacccaacaa
     3481 gtggcggctg tcgaggcaaa agcagaacaa cctatcacac tacctgaaaa gttgagcata
     3541 ttaatcgcgg atgatcatcc gaccaatagg ctattactca aacgccagct aaatctatta
     3601 ggatatgatg ttgatgaagc cactgatggt gtgcaagcgc tacacaaagt cagtatgcaa
     3661 cattatgatc tgcttattac tgacgttaat atgccgaata tggatggttt tgagttgact
     3721 cgcaaactcc gtgagcaaaa ttcttcctta cccatctggg ggcttacagc caacgcacag
     3781 gctaacgaac gtgaaaaagg gttaaattgc ggcatgaact tatgtttgtt caaaccgttg
     3841 actctggatg tactgaaaac acatttaagt cagttacacc aggttgcgta tattgcacct
     3901 cagtatcgcc accttgatat cgaggccctg aagaataata cggcgaatga tctacaactg
     3961 atgcaggaga ttctcatgac tttccagcat gaaacgcata aagatctacc cgctgcgttt
     4021 catgcactag aagctggcga taacagaact ttccatcagt gtattcatcg catccacggt
     4081 gcggctaaca tcctgaattt gcaaaagttg attaatatta gccaccagtt agaaataaca
     4141 cctgtttcag atgacagtaa gcctgaaatt cttcagttgc tgaactctgt aaaagaacac
     4201 attgcagagc tggaccagga gattactgtt ttctgtcagc aaaatgacta aatagcggct
     4261 cccacaatgt tcaaatgtgg gagctattta ccagcacatc ttacatttat gatgagaact
     4321 cctggcgaat ttgttcccca tgctggtcga gcgttgccgc tcccggcata acatgcgggt
     4381 ccgcgcagcc gctgattttt atcggattac ccggcatcat tattcccccg gcttcaatca
     4441 acatatttct cgcctgagtt tgtggtaaat taatggcctc agccacactt aataacggcg
     4501 cgacgggcac accaacttca tgtattctgg ctaaccaaac ttcagctacc tgcgttttta
     4561 acgtccgctc aatatattct ttaagaatag cctggttatg tacgcgtaaa atattgctgc
     4621 taaatcgggg atcattaacc agttccgtaa gctccagtgc ctggcataac gcagaaaaaa
     4681 gcttgtcatt accacaacaa atcgtaatcg gcttatcctg agtatcgaaa acatcaaaag
     4741 gtgccatgta gggatggcga tttcctaaac gttgtggtga cttcccagtc gcgatatatg
     4801 ccatcagtcc atgctccaga aaactcagag tggcatcaaa catcgctata tcgacatgcg
     4861 cccctctctg gctcttttcg cggccataaa gtgcactcac tattccactg aataaataaa
     4921 caccgccaca gagatccgca agagaggtac caacgcgcac tggcggagca tcagggtatc
     4981 ctgtttccat cattatcccg ctcattgcct gaatgatggt atcgtaggca ggagcatctt
     5041 ttagcggacc ggtatgtccg aaacctgacg atgaagcata tataaggcgc gggttgattt
     5101 cttgtaacct ttcccatgaa aacccgagtt tttccattgt acctggacga aaattctcag
     5161 ctaatacatc agcttgttta agcatattta taaatatact tttatcgtga tcattcttta
     5221 aatcaagaac cacactctct ttgccatgat taataaaact gtaatagagt gactgtccat
     5281 ccacataggg accaaatgtg cgggtatcat caccatgacc cggcggctca actttaatta
     5341 cccttgcacc catattacaa agaagttgag ttccgaaagg cccattaagg acatgcgtca
     5401 tatcgataac taataagcct tcaaacggcc ctttgcattc attatttgtc atttgcttat
     5461 gccttgaaaa aagccgaagg tgaaaagaca cttcggcatt tatgtaaaat caataacatg
     5521 attaattatg aaaccagtcg actgacataa atccacaaag gagccgtgac gacaaaaccc
     5581 agtacgctca cagccaatga cgctgtacca gtgcgggtgt agacattaaa ccggctggca
     5641 ataatgattc cggagaatgc cggcggtaat gcgcctgcca gtaccatcat ctgcaaatgt
     5701 tcgctattca aatgacatgc cataccgacg agaagcagcg ccagtggcat cagaatcagc
     5761 ttcaggaaag tgttataagc aatttcggca ctgaactcga atttatgtgc agccagagtc
     5821 aaccccgcag cgaataccgc tacccctgag ttagccttcg caatcagatt aaaggttggg
     5881 tcccatgctg ccggaatttt taccccaacc aataccagga tcgttgccag aacaggagcc
     5941 catactactg gctcctttgc cgcagaaatt aatgcgctca gattactatt cttcttacca
     6001 tccgctcctg aggaaggatt cagcaaatac agaccaatag gaatagtaat tgcgttaaca
     6061 ataatagaaa taattgctac cactaaacct gttgatacgg aatcaccata aataggatcg
     6121 agaactgcaa accccaggaa tccaatggta ggtgaacctg caattaatgc acatactgct
     6181 gcttctgcat gggtacgttt aaaaaatttg tagcaaccga accaggagaa aaagaaacat
     6241 ccgacaataa ccacaagtga tacaagggtc aggcgagtgt ccgcaaaaat catttccctg
     6301 tttgccctgg taatagaaac aaatagagcc gcaggaagag cgtagtttaa taccagttta
     6361 ttaaatgccc gggcttgatc ttctgaaaaa gtttctcgtc tgccgctaaa ataacccaac
     6421 agcatgatta cgataatagg caataaatcg ccaataaaaa atgttagcat aatagtattt
     6481 ccgataaggg tcccctcacc atcgcataat aagttggcaa gaggagacga tgtcagtatt
     6541 taattaccag cgacttgttt tgggttaagt ttggtaatat ggccactttc agttcctgct
     6601 gctgggtcga tgaccacatt aataatggtc ggtttgcgcg actgaattcc ggtggttaaa
     6661 gcatgacgaa gttcatctgt cgtggtgacg ttatagccaa cgccacgaaa cgcatccatt
     6721 aatttgtcat accttgcatg gtgcaacaga tccgttggcg atggtgcacc agcgccgctg
     6781 aggtcaacac cgtctcctct gtagatgccg ccattattaa aaataacgat cgtcaccggc
     6841 aggttatatc gacaaatcgt ttcaatctcc atcccactga aaccaaaagc actatcacct
     6901 tcaatagcga caaccggaga accagaggtc acgctagcac cgatggcata gcccataccg
     6961 atgcccatga caccccaggt gccacaatcc agacgacgac gtggtttata catatcaata
     7021 atatttcgtg cattatccag ggtatttgcc ccttcattaa ctaaataaat atcctggttc
     7081 tcgcgcaaaa catcgcgcac ggcactcaat gcattaaagt aatttaatgg ttgggtatct
     7141 gtacttaact tttcatgcat tttttgtgca ttttgctgct tgtggatatt taaaatatcg
     7201 cgccacacca gtggagtcgt aaatgtgttt tgtttcaatt ctgccagcat accttgcata
     7261 ctagatgcaa tatcgccaac gactggcaca gcaatggggc ggttgctgtc aatttcctgc
     7321 ggttcaatat ccagttgaat aaactgtgta tctgccgccc atcctttttt accgtgtgcc
     7381 agtaaccaat tcagtcgtgc accaacaagc atgacaacgt cagcatttgc cagggcaaac
     7441 gaacgcgcag ctgccgcaga aagtggatgc gtatcttcga ggatcccttt cgccatagac
     7501 attggcagga atggaatctg ggcactttca ataaattcac gaagctgttc atcagattgt
     7561 gaatacgccg cgcctttgcc aaggataatt aatggtcgtt cagcttttgc taaaagcgaa
     7621 attgcgctag tgactgactt cgggcatggc aataatgctg gcgagggatt ttcaacttta
     7681 acaatcgtgg ttaacgcttc gtctttttcc atcgtcgcgg ccaggacatt tgctggcaaa
     7741 tcaagataaa caccgccagg acgacccgat actgagaccc ggatagcgcg tgccaatgca
     7801 atgccaagat cctgcggctg attaacgcga aatgctgctt tggcatacgg ttttgccgca
     7861 ttcatttggt ccagctcttc ataatcacct tgctggagat cgacgatcgc gcggtcgctg
     7921 gagccgctaa tcatgatcat cggaaaacca tttaccgttg cattggccaa tgcggtcaaa
     7981 ccgttgagga atcctggcgc agacactgtc aggcagatcc ctggtttttg ggtaagaaaa
     8041 ccgcttgctg cagcggcata gcctgccgac tgctcatgac gaaaaccaat ataacgaatg
     8101 ccttccgcct gggcatggcg cgccatatcc gtcacaggaa tacctacaac accataaata
     8161 gtgtcaatat tattctgttt taatgcttca acgatgatat gcataccatc tgtcatttga
     8221 agttgatctg acatgattaa taacctcgaa atttttataa attgggagcc agaagccccc
     8281 gttaatatca gatggcgtgg ttttgcttca ttgcagcaat ttcatcgtcg ctataaccca
     8341 gttccagcaa tacagcagcg gtatgttcac ctaatagcgg cgcagattta atatccggcg
     8401 taaaggcaga gaatttcatt ggacaaccaa ctgtcagata ttttccacgc aacggttgtt
     8461 ccacttcgac aacactgcca ctttggcgca aagagggatc aagtgaaatt tctttcatac
     8521 ttaaaaccgg tgcacaagga atatcaaact gagtcaaata ggccacagct tcatgtttat
     8581 caatagtgac agtgtatttt tcgatttcag caaaaatatc gaaaatatgt ggctgtcggg
     8641 catgggctgt actgtatgcc ggatcggtaa tccattctgg tttgccgatg gatttacagg
     8701 tgttttccca gttttgctcc tgaatagtga aataaatata ggcgttagga tcggtttccc
     8761 agcctttaca tttcaggatc cagccaggct gaccgccacc acccgcatta ccaccgcggg
     8821 gaactgcatc accaaatgtg ccattcggat actgcgggta ttcttccaga taccccaact
     8881 tatcgagacg ctgctggtcg cgtaatttca cgcggcaaag gttcaacacg gcatcctgca
     8941 ttgacatggt gactcgttgc ccacgccccg ttttttcgcg atgcagcaaa gcagcaagta
     9001 aaccgatcag caaatgcatt cctgtgttgc tgtcacccaa cgctgcagcg cttaccagcg
     9061 gcggaccgtc ccaaaaacct gtagtagatg ccgcgccacc cgctgcctga gcaacgtttt
     9121 cataggcttt tacattcaca taaggcgaac actcatcaaa ccctttgatc gaaccaaaaa
     9181 tcagacgtgg attgatttct tgaatatgct cccaggtgaa gcccatatga tcaatggccc
     9241 ctggatgaaa gttctcgact aagatatcag cttcgcggat cagcttttcc attacctctt
     9301 tgccttccgc tgttttggta tttaattcaa tagaacgttt gttactgtta agcatggtga
     9361 agtaaagcgc atcgatatca ggaatatctc gcagctggtg gcgcgttacg tcaccaacgc
     9421 cgggacgttc aattttaatg acgtcagcgc caaaccaggc cagcatttga gtacaagatg
     9481 ggccagattg cacaccggtg aaatcgagaa ctttaattcc ttgaagtgga gttgacatag
     9541 caatacctat tagttaatta aatatgtgtt aatttagcgt ttatttaagg cataacgcct
     9601 ctataaataa tctgcaaaat atcgtcgtag ctgcactcga tatttttaaa ttcctgtatc
     9661 aagtgctgaa aatcatttca tcgctacttc caattccttt ctgcatggta agtagtgagg
     9721 acgaaaagaa gaatataata aatattggtt cgcggtaatc agagagagcc gcgacatttg
     9781 tgatgttatt cttacaactg taagaagtgt cagtgatgta caagtaaggg tatgattaca
     9841 ctatttaaag cgtaccataa aatataacat aataaatgat ttgtgataat atgggattat
     9901 tgcggtttac tttgaggaag ataaaatgcg agtttataga accgcgagcg ccaaataatt
     9961 ttatgaccac aacctgtcgt attgtggtca taaaaaattg tcgactggag ggagttaaat
    10021 cctttacctc aatatattgc ctgtcaggtt tagttcacaa acagtgcttc gctatcgacg
    10081 atgattccat cttccgcacc tttaagcgct aggtttgcct cgtagtattg ctttttatca
    10141 agcagtttct gcgcatcagc aagtgcatta cgagtctgtt taagaggcat cagatattgg
    10201 ttttccatta caccaacacc ggccaggcga agttcttcca ttgcgccctt tttatcccct
    10261 ttcgccattt tttcattggc aatttttatc gcggcttctt tttcgggtgt tgctacataa
    10321 ctctcagaaa ttccgacaga tgcattaatg acaatatatt ggtcatcatt gacgttggtc
    10381 tttttacctg gcttagcgaa ctttgcccat tcggtactat catctgacag caaagcggaa
    10441 gcttcattgg tcagttcttt tgccttttcc ggatcgccat gaaacagagc caggcgcgct
    10501 acctgcacgt cacgcatcgc atataaacct tgttcagaga tacgctctgc tgctgccgtt
    10561 ttctgggttt gcttaacttg ctgagtttgc tgttgtgccg ctaccggtgc attatcagca
    10621 gcccatacag ttgaagatgc cagaattgct gtgaccatcg tagccataat taaacgtttc
    10681 atacttacct cattctttaa cagttttaga aaacgccgcc atcatattgg gtgagcatga
    10741 aatgagaaat cagagagcaa cggtcatttt gaaagggtta tcttacagtt gtagttatta
    10801 tcgcaagaag tattttagtc ttacaattgt aggaatatgc ttcctgtcac agagaact
//
LOCUS       DAZFJL010000155        10712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFJL010000155 DAZFJL010000000
VERSION     DAZFJL010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.157"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..582
                     /locus_tag="ACHJ8C_004020"
     CDS             <1..582
                     /locus_tag="ACHJ8C_004020"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFO5553799.1"
                     /translation="LKKSLQKAVRQKEGVETARNELDECLRNEPVKPVRVHLTVTDPT
                     PEALLKELGQYSSLGITSDEGSALHESIMRRKIAIPNTLWCGDDYRVSRASTGITDIK
                     DPRLGMLLMTQPEPHDSTLKSLGNTIRSTGIYARTLTMDLTLLTKLIDIDELVSGDES
                     DLEKFYAIQKKYLLAGIERRKKKSTPYLYDFCT"
     gene            587..1150
                     /locus_tag="ACHJ8C_004021"
     CDS             587..1150
                     /locus_tag="ACHJ8C_004021"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFO5553800.1"
                     /translation="MKRLRDVGRDVKYLMQPGGKLYHYNDWAARYCEHTARSAAVMQL
                     FITPDSTVITLETLEAALLISEWHLNHFIVKMDAVRGPSHSERVIKWLENNLVKNGSY
                     DFLRRDMMQDIHRSLRKKADLEPVLEQLADEGKVQLWEDASGRHYVKYLASEMTPSEL
                     DEKHRVMKGIPHYHGGGSAISRVPLRE"
     gene            1447..2124
                     /locus_tag="ACHJ8C_004022"
     CDS             1447..2124
                     /locus_tag="ACHJ8C_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001614376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO5553801.1"
                     /translation="MKSPYDLTEREFKFLQQEAEDKYDSRISSYILRRSLAVISNYLE
                     NYNRVFALRADLRFAQSHVPGEPDMPTCFQKDDEKAITRAMESLKSQLREEHQRSGRA
                     GECAPLGYIWARERVTGEHPHYHLVLLFDREVYAYLGNYTKADTDNMGVRIQKAWCSA
                     IGLDYPEYAYLPHFPKNNSIWFTRHDALTLSDDYYDFLLRVAYLSKDYSKDFYDGYRN
                     FGTSQLI"
     gene            complement(2213..2686)
                     /locus_tag="ACHJ8C_004023"
     CDS             complement(2213..2686)
                     /locus_tag="ACHJ8C_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001614377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553802.1"
                     /translation="MFTIETAREEANRFRKILETCDPSTTYLVPHGFPVMSCKLCSML
                     LSFHFLQLWPELELKGVTGVTGKNAAITHFWLEVEDYVIDITGDQYNIINARKLNENI
                     VRNRPFMPVHVAHRKDSYLYDLFKIQGTELLTYGFPTIGNDFIDEMECDYRQLVG"
     gene            complement(2887..4128)
                     /gene="intA"
                     /locus_tag="ACHJ8C_004024"
     CDS             complement(2887..4128)
                     /gene="intA"
                     /locus_tag="ACHJ8C_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004929091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO5553803.1"
                     /translation="MARKTKPLTDTEIKAAKPKDADYQLYDGDGLTLLIKSSGSKLWQ
                     FRYYRPLTKQRTKQSFGAYPAVSLSHARKLRAESRVLLAKDIDPQEHQKEQVRNSQEA
                     KTNTFLLVAERWWNVKKASVTEDYADDIWRSLERDVFPAIGDISVTEIKAHTLVKAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LPQLMQTMRTASISMSTRCLFMWQLLTITRPAEAAEARWDEIDFSAKEWKIPAARMKM
                     NRDHTVPLSDEALSILEMMKSLSGGREFIFPSRIKPTQPMNSQTVNAALKRAGLGGVL
                     VSHGLRSIASTALNEEGFPPDVIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWAYF
                     VKKADSGRLTDVELTTLKLTG"
     gene            complement(4491..5663)
                     /locus_tag="ACHJ8C_004025"
     CDS             complement(4491..5663)
                     /locus_tag="ACHJ8C_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5553804.1"
                     /translation="MGKKEQGSLSLPRGVTIRQHKTDSTLVITFTYKGVLCREPLSRM
                     EVNARGVKYAERLLGEIKNQIIDGTFEYAKYFPNSQKLKLFGGVKKNKNIKSYLDEYL
                     IICQNRNLSPSTIRGYEKCLSALSALHKFHVSELTPAVLKNWIASRKTKLKTIRNNLS
                     FLRSAIDEAVTDGLLTINPVTLVSASRYHVLDSTPGSDDYEVDPFTPAETAAIYDACH
                     YPEWRNLFRFAFNTGLRSSELCALRWTDIDFIGNTAHVQTASVVGVIKSTKTKAGTRK
                     VQLNSEALAALQAQKPYTALKSDFIFSDPKTRAPWANADAIRKKAWVPTLKKAGVRYR
                     NPYQTRHTFATRHISQGVNLFWLAGQMGHKGPEMLFRHYGSYLAEYDGKTAISAAP"
     gene            complement(5618..5827)
                     /locus_tag="ACHJ8C_004026"
     CDS             complement(5618..5827)
                     /locus_tag="ACHJ8C_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO5553805.1"
                     /translation="MGLDCVPISTYCRNAGETVDAVNKRIQRGIWKEGVHVLKVDGVK
                     ERWVDLMEITKWARKNKDHYLSLEE"
     gene            complement(5836..5982)
                     /locus_tag="ACHJ8C_004027"
     CDS             complement(5836..5982)
                     /locus_tag="ACHJ8C_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553806.1"
                     /translation="MNKAFEQWVHQRYGNRYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            complement(5986..6228)
                     /locus_tag="ACHJ8C_004028"
     CDS             complement(5986..6228)
                     /locus_tag="ACHJ8C_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5553807.1"
                     /translation="MFALIQRGQIYTDSAGYPIKIIRCINNTVLYRRMDGRTLSVKIN
                     DFNELFERINHQEYRQILAETEQEAHLKKLRAMKRK"
     gene            complement(6316..6474)
                     /locus_tag="ACHJ8C_004029"
                     /pseudo
     CDS             complement(6316..6474)
                     /locus_tag="ACHJ8C_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459871.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7049..7270)
                     /locus_tag="ACHJ8C_004030"
     CDS             complement(7049..7270)
                     /locus_tag="ACHJ8C_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553808.1"
                     /translation="MKIQYQDYGAVANIVITSSCLTLQKHNRVVDAVLFNVQGISENR
                     SGFFWVKSSLSGKTRDILRAYKIVQREAK"
     gene            complement(7271..7609)
                     /locus_tag="ACHJ8C_004031"
     CDS             complement(7271..7609)
                     /locus_tag="ACHJ8C_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553809.1"
                     /translation="MSLITQNFRLNALANQYAAALHNHISATSGGHFLINVNGQSIRV
                     NIIGGVKGIRDLIDGYALKAIKENYPQWEAVGIQLLSKCVRGNELTQSGKEIWQSMVN
                     DMSDTVMGNM"
     gene            complement(7609..8355)
                     /locus_tag="ACHJ8C_004032"
     CDS             complement(7609..8355)
                     /locus_tag="ACHJ8C_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO5553810.1"
                     /translation="MNIVTINNKQLPAVEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMTDAKCRNLVNAYSIPTDTHEFMTPDGLLSRRAIVELEPFMKAF
                     HQMMSEAEPRGTRWYHPKMGLFQVIGWEGK"
     gene            complement(8367..8885)
                     /locus_tag="ACHJ8C_004033"
     CDS             complement(8367..8885)
                     /locus_tag="ACHJ8C_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553811.1"
                     /translation="MIGYSTAVRQIDNGNYDKRIPDGLDLIACIMEGIESGWFMPCIE
                     KQIILWRWLVVAVFIAEEQEKNGTVDVQNDKGGVDTAVVYTGKPGSISIYPWSERFAL
                     ANHIEAGAIEKYGPDVGQQMALRMYQDMVVTDEKCGFRLSAMGREGLNILHDSFIELI
                     QKDGMPDMPVMH"
     gene            complement(9172..9381)
                     /locus_tag="ACHJ8C_004034"
     CDS             complement(9172..9381)
                     /locus_tag="ACHJ8C_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553812.1"
                     /translation="MSDLSPLIHEKVQIIMTIENGQVINTRKVHNNEMIASMDTFLWI
                     AKKAGYQVIAPDLEEIGDINSNTNS"
     gene            complement(9381..9740)
                     /locus_tag="ACHJ8C_004035"
     CDS             complement(9381..9740)
                     /locus_tag="ACHJ8C_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553813.1"
                     /translation="MACTTIYKDRRRMVTAALRRMQRKTGSNLLVVDLPDGEITTIEI
                     TEQFMNQLLLRFEGLTRVEYGRTKGEATIRTAYQNAIGINQHTEYLTESGKLIVDGLL
                     KEVVDYVKEKYISGGIN"
     gene            complement(9925..10116)
                     /locus_tag="ACHJ8C_004036"
     CDS             complement(9925..10116)
                     /locus_tag="ACHJ8C_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553814.1"
                     /translation="MITPLNILEEVAAQIKENTSMLEFIFKNSPDQGETDDYLCCLIR
                     SMNKTCEMAYEYIETLRNE"
     gene            complement(10113..10304)
                     /locus_tag="ACHJ8C_004037"
     CDS             complement(10113..10304)
                     /locus_tag="ACHJ8C_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553815.1"
                     /translation="MKMFKGLTNEPETAFHHIAVLLEAGLIISASGDEECDELSDDIF
                     LLAQQYARSACDAFKEQRT"
     gene            complement(10306..10710)
                     /locus_tag="ACHJ8C_004038"
     CDS             complement(10306..10710)
                     /locus_tag="ACHJ8C_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5553816.1"
                     /translation="MSNLNHFEKESFALNIANLVGAARTFHLIDGDRSKELGFEVMEF
                     VHEYALSATYPLHNHIINKQPNKIRTQREACGLTTAELARLLDLDEEIIIQWESGEYE
                     PTISMLIPLANVLGCDPMLLLTDAYKQNQEEA"
BASE COUNT         2625 a         2480 c         2440 g         3167 t
ORIGIN      
        1 cttaagaaat cgcttcagaa agcagtgcgc cagaaagagg gagtggagac ggccagaaat
       61 gaactggatg aatgtctcag aaatgaaccg gttaagcctg taagggtgca tctgacggta
      121 acggatccca cgccggaagc ccttctgaaa gagctggggc aatactccag cttgggtatc
      181 acctctgacg agggaagtgc tctgcatgaa agcatcatgc ggcgtaagat cgcaattcct
      241 aatacattgt ggtgtggtga tgattatcgg gtcagtcgtg cgtcaacggg tatcacagat
      301 attaaagatc cccggcttgg tatgctgttg atgacacagc cggagccaca tgacagcaca
      361 ctgaaatcac tgggtaacac aatacgatct acgggaattt atgcgcgtac gttaaccatg
      421 gatcttacgt tgctcaccaa actaattgat attgatgagc tggtttctgg cgatgagtcc
      481 gacctggaaa agttttatgc cattcagaaa aaatatttac ttgccgggat tgaacgcaga
      541 aaaaaaaaat caaccccgta tctgtatgac ttttgcacct gatgcaatga agcggttacg
      601 tgatgttggc agggacgtta agtatctgat gcagccgggg ggaaagctgt accattataa
      661 cgactgggct gcacggtact gcgaacatac cgccaggagt gctgctgtga tgcagttgtt
      721 tatcactcct gactcaaccg ttattacgct ggaaacactg gaagctgcat tgttaatatc
      781 ggaatggcac cttaatcatt ttattgtaaa aatggatgct gtccgagggc cttcccattc
      841 tgaaagagtt atcaagtggc ttgaaaataa tcttgtcaaa aatgggtctt atgatttttt
      901 aaggcgagac atgatgcaag acatacatcg ttcactcaga aaaaaagctg atcttgagcc
      961 tgttctggaa cagcttgctg atgaagggaa agtccagttg tgggaagacg caagtggaag
     1021 gcattatgtc aaatatcttg cttccgaaat gacaccttca gaactggatg aaaaacatag
     1081 agtaatgaaa ggtataccgc attatcacgg gggaggtagc gctatcagtc gcgtcccttt
     1141 gagagaataa cttcagagtg atttttatga tttatcaggt ggttatgcat ctaataaccc
     1201 ttagtgtgct gcgggtttcg ttgttgagag tgggattatt atccgtattg attagtcggt
     1261 atatgtaata tacctattgt tgataggtgc tcttacgtca atacactaaa cgtcggtata
     1321 tgccagcata ataatcaaac ctgtatatca gcagagataa tacatccctg taggggagcc
     1381 tgtacgctta attgggtagc agcttgctac ccaattatat aatgaatttc gctaaaagag
     1441 attattatga agtcaccgta tgatttaact gagagagagt ttaaattctt gcagcaggaa
     1501 gctgaagata aatatgattc cagaatatca agctatatcc ttcgtcgatc acttgctgtg
     1561 atatctaatt atctggaaaa ctataaccga gtatttgcat tacgtgctga tctgaggttt
     1621 gctcagtccc atgtgccagg agagccagat atgccgacat gctttcagaa agatgatgag
     1681 aaagccatca ctcgggctat ggaatccctt aaaagccagt taagggaaga acaccagcgt
     1741 tctggcaggg ctggtgagtg tgcgccactc ggctatatct gggccagaga gcgtgttacc
     1801 ggggaacatc ctcactacca tctggttctg ctgtttgaca gagaggtcta tgcatatctg
     1861 gggaattaca ctaaagcgga tactgataat atgggcgtcc ggatacagaa agcctggtgc
     1921 agcgccattg ggcttgatta tcctgaatat gcttatctcc cgcattttcc gaagaacaac
     1981 agcatttggt tcacacgaca cgacgcgtta acgttaagtg atgattatta tgactttttg
     2041 ctacgggtgg cctatctgag caaagactac agcaaagact tctatgacgg ttatcgcaac
     2101 tttggcacaa gccagcttat atgacggata ctgtaaggcc agtagaactt atgatgcaga
     2161 cttgccgata aaagtgcagg tctgcaacta ttttctggtc ctgtgcccac aatcacccga
     2221 caagctggcg ataatcacat tccatctcat caataaaatc attaccgatt gtcggaaacc
     2281 cataggttaa gagttctgtc ccttgtattt taaataaatc atagagatag ctgtcctttc
     2341 gatgagcgac gtgaacaggc ataaagggcc tgtttcttac gatattttcg tttagtttac
     2401 gggcgttgat gatgttatat tgatctccgg tgatatcaat aacgtaatct tccacttcca
     2461 gccagaagtg agttattgcg gcatttttac cggtgactcc agtgacccct ttcaattcca
     2521 gttcgggcca taattgcaga aagtggaagg aaagtaacat tgagcaaagc ttgcaactca
     2581 ttaccgggaa gccgtgaggt acaaggtagg ttgttgacgg atcacatgtt tcgagtatct
     2641 ttcgaaacct attcgcctct tcacgtgcag tctcaatggt aaacaatatt atccccctga
     2701 tttagcatat gagtgtttgt aagcatacgt accaatggca ggctccttag gatttattct
     2761 tcgtagtgtg agtcaatgct gtccggtcaa atcatacgtg aagcctaggg agttagactt
     2821 tatataacag gattgcgcca ttaccgcaca ctcctgtttt tcgcttgtgt ggattaagaa
     2881 ttggtctcat ccagtgagct ttaatgttgt taattcaacg tcagtgagtc tgccactatc
     2941 tgcctttttc acaaagtaag cccaccactg catcatcgga cgacgttgct caaggtaatc
     3001 actgcggttg taagcgcgac gtacctcatt tttgtctaca tgagccagtg ctgcttcaat
     3061 gacatcaggc gggaatcctt cctcattgag tgccgtacta gcgatagagc gtaaaccgtg
     3121 tgatacgagt acgcctccta agccagcacg cttaagtgcg gcattaactg tttggctgtt
     3181 cattggctgg gttggtttga tgcgactggg aaagataaac tctcggccac cactgagtga
     3241 cttcatcatt tccagaatag aaagagcctc atccgatagt ggaaccgtat ggtctcggtt
     3301 catcttcata cgagctgctg gaattttcca ttctttagca ctgaaatcga tctcatccca
     3361 tcgtgcctca gcggcttcgg cagggcgggt aatggtgaga agctgccaca tgaacaagca
     3421 ccgcgttgac atactgatac ttgctgtacg cattgtttgc attaactgtg gaagctgatc
     3481 cggacggata ctcggcatgt ttttcttttg cggtttctcg aacgcttttc cgatatttac
     3541 actgggaact gcatcaatca ggcctgtgtt ctgcgcataa atcatgactt cgttaatacg
     3601 ctgacaaagg cggcgaacag tctctaatgc acctctggcc tgaaccggct gaacagcttt
     3661 aaccagagta tgagccttaa tctcagtgac gctgatatca ccgattgctg ggaaaacatc
     3721 tctctcaagc gagcgccaga tatcgtcggc atagtcctct gttacgctgg ctttcttcac
     3781 attccaccaa cgctcggcaa ctaacaagaa ggtgttggtt ttggcctctt gagaattcct
     3841 cacctgttct ttctggtgtt cctgaggatc aatatctttt gccaacaaaa ctcgagattc
     3901 agctctgagt ttacgcgcat gagaaaggga gacagcaggg taggcaccga agctctgctt
     3961 ggttcgctgt ttggttagag gccgatagta acggaactgc cagagcttac taccgctgga
     4021 ctttattaac agggtcagcc catcaccatc ataaagctgg taatcggcat ctttaggttt
     4081 ggcggctttg atttccgtat cggttaacgg cttggttttt cttgccatgg ggagtctcca
     4141 tgcgtttagg cccaacgaaa acaatagagc ttttcgttgg gcctatcaat gggcctaaaa
     4201 ggttcggatt taattagttc tcttcggact tcgcgggaca aattgaagac acaaaaaagc
     4261 ccgcagggct tgtgtcgtgc gggctcttag gacttcatcg gatgactctg gtaatcaccg
     4321 atggagaatt ttggtggagc tggcgggagt tgaacccgtg atcgtgatta atccaattta
     4381 ttgaatatga acgatttatt ttttcaaaca aggaattatg tgcattttac gtacatattg
     4441 ttgtccctgt aacgtcctga ttctgtacaa cattttgaaa tattttgccg ttacggggct
     4501 gcagaaatcg ccgtttttcc atcgtattct gcaaggtaag agccgtaatg tctgaacagc
     4561 atttccggtc ctttatgccc catctgacca gcaagccaga acaggttaac gccctggcta
     4621 atgtgccgag tggcgaacgt atggcgtgtc tggtacggat tgcgatagcg tacaccagcc
     4681 tttttaaggg tcggcaccca ggcttttttt cggatcgcgt ccgcgtttgc ccaaggggcg
     4741 cgagtttttg gatcgctgaa tataaagtcg ctttttagcg ccgtgtaggg cttttgtgcc
     4801 tgaagagccg ccagcgcttc gctgttcagt tgtactttgc gagtgccggc ttttgttttg
     4861 gtgcttttga ttaccccgac tacacttgca gtctggacat gcgctgtgtt gccgatgaag
     4921 tctatatctg tccagcgcaa tgcgcacaat tccgagctgc gcagcccggt attaaaggcg
     4981 aagcgaaata gatttcgcca ttccgggtaa tgacaggcgt catagattgc tgctgtctcc
     5041 gctggcgtaa agggatcgac ctcgtaatca tcactgcctg gtgtgctgtc taatacgtga
     5101 tatcggctgg cgctgactag cgtgacagga tttattgtta acaaaccatc ggtcaccgct
     5161 tcatctatgg cgctgcgcag aaacgaaaga ttattgcgga tcgttttcag ttttgttttt
     5221 ctgctggcga tccagttttt tagtactgca ggtgtcagtt ctgatacatg aaatttatgc
     5281 aatgctgaca gcgccgacag gcatttttca taacctctaa ttgtcgatgg tgacaggtta
     5341 cggttctggc agataatcag atattcatct aggtaagatt ttatattttt gttttttttc
     5401 actcccccga acaacttcag cttttgggag ttggggaagt attttgcata ttcgaaggta
     5461 ccatcgataa tctgattttt tatttctccc agcagacgct cggcgtactt cacaccgcgc
     5521 gcgtttactt ccattctgga gaggggctcc cgacacagaa cccctttata tgtaaaagtg
     5581 ataaccagag tgctatcagt tttatgctgc cgaatagtta ctcctctagg gagagataat
     5641 gatccttgtt ctttcttgcc cattttgtaa tctccattaa gtcgacccaa cgttctttaa
     5701 ccccgtcgac ttttaataca tgtacccctt ctttccatat tcctctttgt atccgtttgt
     5761 taacggcatc aaccgtttct cccgcgttgc ggcagtaggt tgatataggt acgcaatcca
     5821 gccccaaagt tcacctcaca caacactcag cccacggcag tggcaccaca cttcaaacat
     5881 tcgtttcaca atttcacgac agtagaaacc gtcaacatct cgtgtcaggt catagcgatt
     5941 gccgtaacgc tggtggaccc attgttcaaa tgctttattc attctttact tccttttcat
     6001 ggcccgtaat tttttcagat gagcttcctg ttctgtttct gccagaattt gtcggtattc
     6061 ctggtgattg attcgttcaa acagttcatt aaaatcgttt atttttaccg acagtgttct
     6121 cccatccatt cttctgtaca acacagtgtt gtttatgcag cgaataattt ttatcgggta
     6181 gccagcacta tcggtgtata tctgaccacg ttgaatcaga gcgaacattc ctttatcccc
     6241 agcggaaaag cgaatacaga ataaatgcca ccgctattgc aactcctaca gcggtgaatg
     6301 cttcaggcca attcatcatt tcacctccac gccgatcccg gcaataacac aatcccgttt
     6361 gatagcttct ttcacccagc gtttataggt ttccagatgg aattttgcgc tttttccggt
     6421 accgctccag aatgcctttg atgtggtatc gggcagggtg atggtcaggc ctttcttctt
     6481 gatgtctcca gaacttttgc aaaaatttaa tgcgtcgcgt agttctttaa cgcacgcata
     6541 gttttctgct ttctctggat cgccggtacg gtgttcagct tcttgtctgc accatttgat
     6601 gactttctca gcggctgcgt ggagacgctg acgttgccag tcgtatgtgt cgaccagccc
     6661 atatgcgtga tcctttgctt tgcttaattc agctacgagg cattcggaat caatcagatt
     6721 attttccgca gcggaaagtg cttgtttcag tcgctcgatt tcttcagcca tgtaatagcc
     6781 tgttttactc caggtatcga catcatcgtc actcatatcc agttccatcg acgccatcag
     6841 aacggcgtcg tgatagttct ggctaccgca ggtgattgca acggcgtagg agtcactatt
     6901 ttctcgctta tggataagta caactgggtt tttgatcttg cccaccccgc acctcagtaa
     6961 ttcattaaaa atttatattc aatcagcgta ccgaaaacga cggacgccag cagcagacta
     7021 aacagcatgg acaggaggaa tgacttcatc actttgcctc ccgctgtacg attttgtagg
     7081 cgcgcaggat gtcgcgtgtt tttcctgaca acgatgactt aacccagaaa aagccgctgc
     7141 ggttttcaga gatcccctgt acattaaaca gcaccgcatc aacaacgcgg ttatgctttt
     7201 gcaatgtcag gcaggaactg gtgatcacga tgttcgctac ggcgccatag tcctggtatt
     7261 ggattttcat ttacatgttc cccataacgg tatcgctcat gtcgttgacc atgctctgcc
     7321 agatttcttt gccgctttgc gtcagctcgt ttccacgaac gcatttgctt aacagttgaa
     7381 taccaacggc ttcccattgg gggtaatttt ctttgatagc cttcagagca taaccatcga
     7441 ttaagtccct gatgccttta accccaccga tgatgtttac tcgtatggat tgcccattga
     7501 cattaatcag gaaatgacca ccgctggtgg cggatatgtg gttgtgcagt gccgccgcat
     7561 actgatttgc cagcgcattg aggcggaaat tttgtgtaat gagtgacatt atttaccctc
     7621 ccaaccaatc acctgaaaca gccccatctt cgggtgatac cagcgggtac cacgtggctc
     7681 tgcctctgac atcatctgat gaaatgcttt catgaatggc tccagctcca cgatagcccg
     7741 gcgcgaaaga agaccatctg gtgtcataaa ttcgtgcgta tcggttggga tgctgtaggc
     7801 attgaccaga ttgcggcact tagcatcagt cattccgctt ttggcgacca cctggcgata
     7861 accgacatat ccggcgcgca tatttccgcg tttgatggtt tccaccgctt ctgtgactgt
     7921 ttcgagctgt tcttccacat gattcaggcg cttctgttga cgaacagcgt cggcggctat
     7981 cgcagcgatc atctcgattt ccgttagcgg tgtgcgagtg cggaagtagc tgttaaccag
     8041 ttcgcgctga acctgccagg caagggcgtc attgaatggc ttcgtcaaca tcaggtaacc
     8101 tgattcaaaa agtacaatcc ctgatggtgc aaatttagag aatgttcctt ccgggaggtc
     8161 cgtacgtatt acgtccgcac ctaattcggc ataatccaca ccgctgatga aatgctcacg
     8221 gtttcggttg aatgctgcac gcgctgttcc ttccggtcgc tggtggactt catcaatcat
     8281 cgccagcgtc acaacacgct gaccgcgata ttcgactgcc ggaagctgtt tgttattgat
     8341 cgttactatg ttcattttcg tccacctcag tgcataaccg gcatgtctgg cataccgtct
     8401 ttctgaatca gttcaatgaa gctgtcatgt agtatgttga gtccctcccg gcccatcgct
     8461 gacagcctga atccgcattt ctcgtcagtt acaaccatgt cctgatacat acgcagcgcc
     8521 atctgctggc caacgtcagg cccgtatttt tcaattgcgc ccgcctcgat atggttagcg
     8581 agcgcgaagc gctcagacca tggataaatg ctgattgaac cgggttttcc tgtgtataca
     8641 acggctgtat ctacgccgcc cttgtcattc tgaacatcga cagtcccgtt cttctcctgc
     8701 tcctcagcaa tgaacacggc gacaacaagc cagcgccata ggatgatttg tttttcaata
     8761 cacggcataa accagccgct ttcaatccct tccataatgc aagctatcag atcgagcccg
     8821 tccggaattc gtttgtcata gttaccgttg tcgatctggc gaaccgcagt tgaataacca
     8881 ataattcgat taccaaaacg gatgcctgtt aatgtaggtt caggggtaat ggttgaatta
     8941 atcatcaata ctctcctttt ggtttgaagg tttattgttc atttccggtc cttaactttg
     9001 ctgtatcgtt catggctcat tacttcccag ttctggccgc cgtctcggga cagcagccgc
     9061 cagcggcgat taaccctcag actcaggttt ccggagccgt gcatacgaca gggatgaatt
     9121 cgtctggctc tgaactggcg gagaacacgg accgcctgca ggtgcaccca ctcaggaatt
     9181 cgtattgctg ttaatgtcac cgatttcctc cagatcagga gcgattacct gatatcccgc
     9241 tttctttgct atccataaga aggtatccat gctggcaatc atctcgttat tgtgaacttt
     9301 acgggtattt attacctgac cattttcaat cgtcataatg atctgtactt tttcgtgtat
     9361 aagaggggat aaatcagaca tcagttaatt cctccactga tatatttctc ttttacgtag
     9421 tcaacaacct cttttaaaag gccgtccact atcagtttcc ctgattcagt gagatattcc
     9481 gtgtgttgat taataccgat ggcattctgg tatgcggtac ggattgttgc ttctcctttc
     9541 gtccgtccat attcaacacg ggtaagaccc tcaaatcgta ataacaactg gttcatgaac
     9601 tgttcagtta tttctatcgt tgttatttct ccgtccggga ggtcaacgac gagcagatta
     9661 ctacctgttt tacgttgcat ccgccggagt gccgctgtta ccatgcggcg acgatcttta
     9721 taaatggttg tacatgccat ttgttatttc ccgtatgctt ttctgagaaa cagtattgca
     9781 actgaccagt atcctgcatt agccattaat aatgcggttt tataagcgtt tctatttttc
     9841 atgcatcacc attattttct ggttgtggaa atccacgacc agaggccgtc atgtttttat
     9901 gtgtgatttt ttactgagtg ttctttattc gttgcgtagt gtttctatat actcgtaagc
     9961 catttcacag gtcttattca tggagcgaat gagacagcat aaataatcgt ctgtctctcc
    10021 ttggtcgggc gaattcttaa atataaattc aagcattgat gtattctctt ttatttgtgc
    10081 cgccacttcc tcaagaatat ttaatggagt tatcatgttc tttgctcctt aaatgcatcg
    10141 catgcgcttc tggcgtattg ttgtgccagt aaaaagatat catccgaaag ttcatcacat
    10201 tcttcatcac cggaagccga aatgattaac cccgcttcaa gcagtacggc aatgtgatga
    10261 aaagctgtct ccggttcgtt ggtaaggcct ttgaacattt tcatcttatg cttcctcctg
    10321 attttgttta taagcatcag tcaataacaa cattggatca cagccaagaa cattagccag
    10381 agggataagc atgctgatgg ttggttcgta ctctccgctt tcccactgga tgataatttc
    10441 ttcatcgaga tcgagcagcc tggcgagttc ggcggttgtt aagccgcagg cttcgcgttg
    10501 ggtgcggatt ttatttggtt gtttgttgat tatgtgatta tgtaatggat aagtagcaga
    10561 cagggcgtat tcatgaacaa actccatgac ttcaaacccc aattctttag agcgatcacc
    10621 atcaattaaa tggaaagtac gagcagcacc taccagattt gcaatattta atgcaaagga
    10681 ttctttttcg aagtggttta agttcgatat ct
//
LOCUS       DAZFJL010000156        10665 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFJL010000156 DAZFJL010000000
VERSION     DAZFJL010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.158"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            87..800
                     /gene="yghS"
                     /locus_tag="ACHJ8C_004039"
     CDS             87..800
                     /gene="yghS"
                     /locus_tag="ACHJ8C_004039"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO5553817.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAATMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKNQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            832..1590
                     /locus_tag="ACHJ8C_004040"
     CDS             832..1590
                     /locus_tag="ACHJ8C_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553818.1"
                     /translation="MDALQTQTVKSTTAPQPNYIPGLIAVVGCDGIGKSTLTNDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1636..2985
                     /locus_tag="ACHJ8C_004041"
     CDS             1636..2985
                     /locus_tag="ACHJ8C_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO5553819.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNMVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNSTGEKS"
     gene            2985..3821
                     /locus_tag="ACHJ8C_004042"
     CDS             2985..3821
                     /locus_tag="ACHJ8C_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000984967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553820.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLKFWLDGVNRQELLRLIRKQAKGDELTTDERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGHFQDGFRNGKKNIVSFYGNLLRVYLSSPVWWLVNYSLRHSQ
                     LETVNGFIAYRQKQMYILKEIIAKPQLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALLISRFLAAINGLMGDKHDDMVADDMDNRRSYDAPVALDSDIRQRLELLISRFPLYP
                     EQ"
     gene            3821..4381
                     /locus_tag="ACHJ8C_004043"
     CDS             3821..4381
                     /locus_tag="ACHJ8C_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO5553821.1"
                     /translation="MPSRWIFALIIALLAPGAAAHNFVTGKTVTPVYIQEGGELLLNR
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFSQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGLGRVAWQLPEQSSTILVLNKAGRI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            4412..5482
                     /gene="lptF"
                     /locus_tag="ACHJ8C_004044"
     CDS             4412..5482
                     /gene="lptF"
                     /locus_tag="ACHJ8C_004044"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO5553822.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYASVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            5479..6558
                     /gene="lptG"
                     /locus_tag="ACHJ8C_004045"
     CDS             5479..6558
                     /gene="lptG"
                     /locus_tag="ACHJ8C_004045"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO5553823.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWGASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSTFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(6593..7765)
                     /locus_tag="ACHJ8C_004046"
     CDS             complement(6593..7765)
                     /locus_tag="ACHJ8C_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO5553824.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIREGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDVN
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKHRLGGYLIVDEAHSFGVL
                     GAAGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIASQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLCIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(7765..8013)
                     /locus_tag="ACHJ8C_004047"
     CDS             complement(7765..8013)
                     /locus_tag="ACHJ8C_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO5553825.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(8045..8959)
                     /locus_tag="ACHJ8C_004048"
     CDS             complement(8045..8959)
                     /locus_tag="ACHJ8C_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO5553826.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGTPETLLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVQQLVADVRCGSVRMVGIPQPLLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(8956..>10665)
                     /locus_tag="ACHJ8C_004049"
     CDS             complement(8956..>10665)
                     /locus_tag="ACHJ8C_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO5553827.1"
                     /translation="FTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEY
                     QTLKARAEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGV
                     GQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNNPELHVLSHAWFKALPEAD
                     VVLQRPVPNDIAYLQYTSGSTRFPRGVIITHHEVMANLRAISHDGIKLRPGDRCVSWL
                     PFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYEL
                     CQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTLMPCYGLAE
                     NALAVSFSDEACGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIR
                     NEADIPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVT
                     GRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERR
                     GQLIHALAARIQSEFGVTADIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLHVQE
                     SLA"
BASE COUNT         2555 a         2673 c         2785 g         2652 t
ORIGIN      
        1 attaaccatt gtcacaataa ggttgaacgg atattctgag cacggaccac gtaatattca
       61 atacattatt tgctgccggg tttttcgtga atcaacgtaa tatgtcaata attaattcca
      121 cgccagtgcg tgttattgcc attgtaggat gtgatggttc aggtaaatcg accctcacgg
      181 caagcctggt aaatgaactg gcagcaacaa tgccaacaga acacatttat ctcgggcaat
      241 cgtccgggcg aattggcgaa tggatttcac agctccctgt tattggcgca ccttttgggc
      301 gttatctgcg aagtaaagcg gcacatgtgc acgaaaagcc ctccacacca cctggcaata
      361 ttactgcgct agttatctat ctgctttcct gctggcgggc gtacaagttt cgcaaaatgt
      421 tgtgtaaaaa ccagcaaggc tatctgctca tcaccgaccg ctacccgcag gttgaagtgc
      481 cgggtttccg ctttgatggc ccgcaattgg caaaaaccac gggcggtaac ggttggataa
      541 aaatgttaag gcagcgtgag ctgaagctgt accaatggat ggcgtcttat ttgcctgtat
      601 tgttgattcg tcttggcatt gatgaacaaa ccgcgtttgc gcgtaaacct gaccaccaac
      661 tggcagcgtt acaggaaaaa atcgccgtta cgccgcaact gacattcaat ggtgccagga
      721 tccttgaact ggatggtcgg caaccagccg atgaaattct gcaagcgtca ctacgcgcaa
      781 ttcacgccgc gctttcctga tcaccaaaat ggatagggga tgattaaacg aatggatgca
      841 ctacaaacac aaactgttaa gagtacaacc gcgccgcagc ccaactacat tccggggctg
      901 attgcggtgg tcgggtgtga tggaatcggt aaatccacac tgaccaatga cctggtgaaa
      961 tcgctgcaac aacactggca aaccgagcgg cgctatctgg ggctgctctc cggcgaagac
     1021 ggcgacaaaa tcaaacgatt gccgttggtt ggtgtctggc tcgaacggcg actggcggcc
     1081 aaatcctcga aaacccaaag catgaaaacc aaatctccag cgctatgggc ggcggtgatt
     1141 atgtactgct tctcgctgcg aagaatggcg aatctacgca aggttcagcg actggcgcaa
     1201 agtggcgttc tggtggtcag cgatcgcttc ccgcaggctg aaatttcggg cttttattat
     1261 gacggaccgg ggatcggcgt cgaacgtgcg accgggaaaa tcagcatgtt tctggcgcag
     1321 cgcgaacggc gtttatacca acaaatggcg caataccgtc ctgaattaat cattcgcttg
     1381 gggattgata ttgatactgc catctcccgc aagcctgacc atgactatgc cgagctgcag
     1441 gacaaaatcg gcgtcatgtc gacgattggc tataacggca caaaaattct tgagattgat
     1501 tccagagcgc cttacagcga ggtgctggaa caggcacaga aggcggtttc tctggttgcc
     1561 atcgtttctg accgccgaag tttaacttaa ggctggcggg atacttttta ctatcaacga
     1621 taaggtcagg ctgaattggc gggttttaac atcaaacatt ggtttgcaga tggcgcgttt
     1681 cgcaccatta ttcgcaatag cgcctggtta ggctccagta atgttgtgag cgctttgctg
     1741 gggctgttgg cgctctcgtg tgccggtaaa gggatgacgc ccgccatgtt tggcgtactg
     1801 gtaattgtgc aatcgtacgc caaatcgatc agcgatttta ttaagtttca gacatggcaa
     1861 ctggtggttc agtacggaac gccggccttg accaacaata atccgcagca atttcgcaat
     1921 gttgtctcat tttccttctc gctggatatc gtcagcggcg cggtggcgat tgtcggcggc
     1981 attgccttac tgccattcct ttcccattca ttaggtctgg atgaccaaag cttttggctg
     2041 gcggcgctct attgcacgct cattccttca atggcttcct ccacgccgac cggcattctg
     2101 cgtgcggtag atcgctttga tttgattgct gtacagcagg cgacgaaacc ttttctgcgc
     2161 gcagcgggga gtgtcgtagc ctggtatttt gactttggtt ttgcgggttt tgttattgcc
     2221 tggtacgtgt cgaatatggt tggcggcacc atgtactggt ggtttgccgc gcgcgaatta
     2281 cgccgcagaa atatccataa cgccttcaaa ttgaatctgt ttgagtctgc ccgacacatt
     2341 aaaggcgcgt ggagttttgt ctggtcaacc aacattgccc actccatctg gtcggcgcgt
     2401 aactcgtgca gcacagtgtt ggtggggatc gtgttaggac ccgctgctgc cgggttattt
     2461 aaaatcgcca tgacattctt cgatgccgcc ggaacgccag cgggtttact gggcaaaagt
     2521 ttttatccag aggtgatgcg tttagatcca cgcaccacca gaccgtggtt gttaggcgtg
     2581 aagtccggtt tactggcggg cggaatcggt attctggtgg cgctcgcggt gttgatcgtc
     2641 ggcaagccgc tcatttcgct ggtgtttggc gttaagtatc tcgaagcgta tgacctgatt
     2701 caggtgatgc tgggcgcaat tgtgatttcg atgctgggct tcccacagga gtcattgcta
     2761 ttgatggcgg gaaaacagcg cgcttttctc gtggcgcaaa ccatcgcctc aatcggctac
     2821 atcgtattgc tgttcatgtt ctgtcatctg tttggcgtgc tgggggctgc atttgcttac
     2881 ttcggtgggc aatgtctgga tgtggtgctc tcgctaattc cgaccttaaa ggcatttttt
     2941 cagcgccatt ccttgcttta taactcaacc ggagagaaat cctgatgatg agaaaatatt
     3001 tcccgcttga ggcgagcgag cgtctatttg ttgcgattga agaagatgat gttgttgatg
     3061 cacaggtcag tttgccgccg actatcgcac taagttgtac caccgaaatc atccatgata
     3121 attacgcgtt atgcctgaaa ttctggctgg atggtgttaa tcgtcaggag ttactgcgtt
     3181 taatccgcaa acaggcgaaa ggcgatgagc tgacgacaga cgagcgcaaa cagtttaaat
     3241 acatgcgagc gcgctacaag catctgcgtt ttgcgcaacg gctgtattta aagaagcatc
     3301 aggccggatt tttattcggt aaaacgaccg tttttcttgg gcattttcag gatggctttc
     3361 gtaacggtaa gaaaaatatt gtgtcgtttt acggtaacct gttacgtgtt tatttgagct
     3421 cccctgtctg gtggctggtg aactattcat tgcgccatag ccagttagaa accgtgaacg
     3481 gttttatcgc ctaccgccag aagcaaatgt acatcctgaa agagattatt gctaaaccgc
     3541 aactaactgg cagggaattt catgatgtgc gaaaaatcat cagccagcag gtttcttatt
     3601 acgacaccct aagatcgctt gatcctgaaa ataaagaggc gttactgatt tcccgttttc
     3661 tcgccgccat taatggtctg atgggggata agcacgacga catggtggcg gatgatatgg
     3721 ataaccgtcg gtcatatgat gcaccggtgg cgctggatag cgatattcgc cagcgtttag
     3781 aactgcttat ttcgcgtttc ccactctatc cggagcaata atgccatctc gttggatttt
     3841 cgctctgatc atcgcactct tagcgccagg cgcggcggca cacaatttcg tcactggcaa
     3901 aacggttact cctgtttata tccaggaagg cggcgagctg cttctgaatc gcgacgacga
     3961 aattcactac caaaagtgga acagcacaca attggcggga aaagtccgca ttattcagta
     4021 tatcgccgga cgtaagtctg cgaagaaaaa gaactcactg ttaattaaag cggttgaagc
     4081 ggcaaacttt tcgcaggatc gttttcagcc caccactatt gtgaacaccg atgatgccat
     4141 ctttggtact ggctattttg tcgttggcaa aatcgaaaaa aataaacgcc gctatccgtg
     4201 ggcgcaattc gttattgacg gcaatggtct gggacgcgtt gcctggcaat tgccggagca
     4261 gagttcaacc attctggtat tgaataaagc cgggcgaatt cagtgggcaa aggacggcag
     4321 cctgacaccg gaagaggtag atcatgtcat tgcgctggcg cagaaactga tcaatgaata
     4381 aaaaattcaa aggattgagg gcgttagcga gatgaagctg gttgaacact acatcatgcg
     4441 tggtacgcgc cgtctggtgc tgattatcgt cggttttctg atctttattt tcgccagcta
     4501 ctccgcacag cgttatctga ccgaagcggc aaatgggaca ttagctcttg atgttgtgct
     4561 ggatatcgtt ttctacaaag tgctgattgc actggagatg ctgttacctg ttggactata
     4621 tgcttcagtt ggcgtgacgc tagggcaaat gtataccgac tcggaaatta ccgctatctc
     4681 cgcggcgggg ggcagtccgg gacgtttgta caaagccgtt ctttatctgg cgataccgct
     4741 aagtattttt gtcacccttc tgtcgatgta tggtcgtccc tgggcttatg cgcagattta
     4801 tcaactggag caacaatcgc agtcggagct ggatgttcgt cagttgcggg caaagaaatt
     4861 taacactaac gataacggac gaatgatcct ttcgcagacg gttgatcagg ataataatcg
     4921 cctgactgac gcgctgattt atacttctac tgccaatcga acccgcattt tccgcgcccg
     4981 ttcggttgat gtggttgacc catcacccga gaaaccgacc gttatgttgc ataacggtac
     5041 cgcctatctt ctcgatcatc aggggcgtga cgacaacgaa cagatctacc gtaatctgca
     5101 attgcatctc aatccgctgg atcaaagccc taacgtcaaa cgcaaagcaa aatcggtcac
     5161 ggagctggcg cgctccgtct ttccagccga tcatgccgaa ctgcaatggc gacaaagccg
     5221 tggcctgacc gcattgttga tggcgctgct ggccatttca ttaagtcggg taaaaccgcg
     5281 gcaggggcga ttttcaacgc tactgccact gacgttgctg tttgttgcca ttttttatgg
     5341 cggcgacgtc tgccgtacgc tggtggctaa cggcgcgatt cccctcattc ctggtttgtg
     5401 gttagtaccc ggactcatgc taatgggcct gctgatgctg gtcgcacgcg acttctcttt
     5461 gctgcagaaa ttttcccgat gaatgttttc agtcgctatt taatccgtca cctctttctc
     5521 ggttttgccg ccgccgcagg gctattgctg ccgcttttta ccaccttcaa cctgattaac
     5581 gaactggatg atgtcagccc gggcggttat cgctggaccc aggcggtgct ggtggtgctg
     5641 atgaccttac cgcgcacact ggtcgaactt tcgccgttta tcgccttatt gggaggtatt
     5701 gtcggtctcg ggcagctatc gaaaaacagt gagcttaccg ccattcgcag cacggggttt
     5761 tctatcttcc gtattgcact ggtggcgctg gttgccggga tactgtggac tgtttcgtta
     5821 ggcgcgatag atgagtgggg ggcgtcgcca ttgcagcaac aggcgctgca aatcaaatcg
     5881 accgccaccg cgttggggga ggacgatgac attaccggca atatgctttg ggccaggcgc
     5941 ggtaatgaat ttgtgacggt gaaatcgctg aacgagcagg gccagccggt gggcgtggag
     6001 atttttcatt accgcgatga tctctcgctc gaatcctaca tttatgcgcg cagtgccacc
     6061 attgaagatg acaaaacgtg gatcctgcat ggtgtgaatc ataaaaaatg gctgaatggc
     6121 aaagaaacgc tggaaacatc tgataatctt gcctggcaat cgaccttcac cagtatgaat
     6181 cttgaagagt tatcgatgcc ggggaatact ttttctgtcc gtcagcttaa tcattacatc
     6241 cattatttgc aggaaaccgg gcaacccagc agcgaatacc gccttgcact gtgggaaaaa
     6301 ctggggcaac ccatcttgac cctggcgatg attttgctgg ctgtgccgtt tacctttagt
     6361 gccccgcgct cgccggggat gggtagccgt ctcgctgtag gggtcatcgt tggtttactc
     6421 acctggatca gctatcaaat catggtcaat ctgggattgc tatttgcgtt gagcgcacct
     6481 gttaccgcgc tcggtttacc ggtagcgttt gtgctggtgg cgttgagcct ggtgtattgg
     6541 tatgacagac aacattaaac ccgccgtggc gcaacaccac ggcgggttca cattactgct
     6601 tcagagtagc aaatgcctgg atgatggcat caatttgctc gcgactgtgg gccgcgttta
     6661 cgctacagcg cagcagcgta atgccagcag gcgcggcagg tggcaggata agattcacat
     6721 aaacgcccag cgaaataagc tcgcgccaga tgcataaacc ttcctctttc gaaccgatta
     6781 ttactggcac taccgggcta atatgtgaac ttaactcgta acccagtttt tgcagaccgt
     6841 catacagatg gttggcgtta tccatcagct tttgccgcag ctctggttgt gaggcaatgg
     6901 ttcttaacga cgagcgcacg gtcgcaatgc aggacggaga aggagaggcg gtaaaaatat
     6961 acgggcggct gccgtagcgc agcacttcca tcgcttccga accgacagca aaaccgccaa
     7021 tcgaggccag acttttactg aaggtgccaa cgataatatc cacatcatct tcaacgccga
     7081 cagcttcggc cagtccgcgc cccgcagcgc ctaacacgcc aaacgaatgt gcttcatcga
     7141 caatcaaata accaccgaga cgatgtttga tatcaacaat ttctgccagc ggcgcaacgt
     7201 caccgagcat actgtagatg ccttcaacga taataatcgc ctctttcgcg cgttcaccga
     7261 ggcggaccat gcgccgttcc agatcgttga cgtcattatg gcggaaacga ataatctccg
     7321 caccgccgag agaacaagcg tcataaatac tggcgtggct gtcggcatcc agtaacacca
     7381 cggcgttatg gtcggcaagt gcgctgatga cacctaaatt agcggtataa ccggttgaaa
     7441 aaacgatagc ggtagggcga ttgaaaaact cggcaatctc tttctctaac gccagatgcg
     7501 gtgcataact gccgtttgcc atacgagaac cggtggtgcc ggtgccttgg gccgctaacg
     7561 ctgcctgacc ttcgcggata gcatcatgat taaaggttaa accgagataa ttattggtcc
     7621 cggccagaat cactttttta ttgccgatcc ggccttcggt ggcggaatac acttcatcaa
     7681 tacaggtgcc aaatggtgtt aacccgttgt cactaaattg caggcgctcg ccagcaaggc
     7741 gcgcatattt atcgtatagc cccattattt attctccagc catggaagta gagtttccat
     7801 taattgcgca ggggttttga catccagcag aatattaata ggaatggaaa tatcgaatct
     7861 atcttccagc atcattaaga gatccatcac tttgatcgac tccaggccaa gatcgttgac
     7921 gagatcgcta tcgggcttaa tttccacacc gttttcgacc aaatcctgta aacaggaaag
     7981 gatgtaatcc attactattt cacgatttac catagaaacg ttaacacacc tttaaacaag
     8041 acaatcaaaa tagcccgttg cgtaatgcgt gttccaggct aatcccggga aaccaattaa
     8101 tatcttcaga aatacggtta ttacttgccg accagtcggc atgggttaat tcacggattt
     8161 tcgaacgagt cagcatcggt tctttacccg ccagacggct caacgcggta ctgatatccg
     8221 caaggcaggt gagtagcggc tgaggaatac cgaccatccg cacggaacca cagcggacat
     8281 cggcaacaag ctgttggaca cgttgccagt catagcctcc ggcgacgcca tcgcataatt
     8341 cataggtttg cgtctgtaca gtttcggcgc ttaaccactg acccactgct tgcgcaaaat
     8401 cggtgacgtg aagaaaagag agcagtgtct ctggcgtgcc aagtcgtggc agcagaccgc
     8461 gcagcatcca gtcaaacagc ggttttaact ctttatcgcc gggaccatac acggctgtcg
     8521 ggcgaaaaac gcccaacgta atttcgtcag ccattgcagt cagccgttgt tcggcgacgt
     8581 gtttggaatt tgcgtaccag gagagttcgg gatggcgcgc cgccagcgaa gagataaaca
     8641 gaaaacgttg gcaaaagccg ctctcttttg ccgcttgcat caggcgcagg ctgccgtcaa
     8701 cgttgcagcg ggtaaaaatc tcttctttgt gcccgcgcac ttgcccggcg caatggacca
     8761 ccgcgctggc tccggcaacc agttcgctaa gcgaatgtgt atcttccagc gaaccgcgca
     8821 cccaagtgag attatcattg acgtgagcgc gggcagtacg cgtcaatgcg cgaacgtgaa
     8881 agccgcgggc gagcaggtta tcaataatat atttaccgat aaacccggta gcgcccgtca
     8941 ccgcgacagt ttgattcatg ccagggattc ctgcacatga agactggcag cataagcctt
     9001 ctgataacgt tttttcgctt ccgcacgggc aggcttgccg gaggatgttc ggggaatact
     9061 gtggggcggc aacagatcga tatccgcggt aacgccaaat tcgctttgga tccgagctgc
     9121 cagcgcgtgg ataagctgcc cgcgacgttc ttcgtcgctg atccgacact ggatctgcaa
     9181 aatgattttt tcctgggcgg taacaaaagc aatcgcatcg ccagaatgaa tttccggctc
     9241 ctgttccgca atgtattcaa tatcctgcgg ccagatatta cggccacgaa taataatcag
     9301 atctttaatg cgtccggtga catacagata accgtccagc agataaccaa ggtctccggt
     9361 gtctaaccag cccgtcgcgg caatctcgtc ttgcgaaacc tggtcgccaa agtacccgct
     9421 catcaggctg gggccggaga tgcaaatatg gcctaccaca cgttccgcga caggtatatc
     9481 tgcttcattg cggatttcaa tgccatgttc cggcaacgct ttaccgcagt tgacgaaagt
     9541 cgatacggcg cgtgtttcag cacccggcgc gacggcttta ccctggtatt cgaggatgtc
     9601 gcgatccact tcgttaacca caaccccgca ggcttcatca gagaagctga cagccagcgc
     9661 attttctgcc agtccgtagc acggcattaa agttttatcg tcaaagttaa cctgacggaa
     9721 acattcagcg aattgatgga gttgttctgc ggatatgggt tctgcgccaa taccagcgac
     9781 gcgccagcag gaaagatcca gttcagcgag atctttttca ttcacgcggc gctggcacaa
     9841 ttcatagcca aacggcggtg caacggaaac ggtgccacga tttttactga tcaatttaag
     9901 ccattgcaga ggacgcatgg caaaatcctg agtgcgcaaa taatctactg aaagctgcgt
     9961 ggcgacgggg gtcaggagaa agccgaccag tcccatatca tggtagaaag gcagccagga
    10021 gacgcagcgg tcgccagggc gtaatttaat cccatcgtgg cttatagcac gtagattagc
    10081 catcacttcg tgatgggtga taatgacgcc acggggaaaa cgggtgctgc cggaggtgta
    10141 ctggaggtag gcgatatcgt ttggaactgg acgctggagc acaacatcgg cttccggtaa
    10201 tgccttaaac caggcgtggc ttaaaacatg taattcgggg ttgttgttat gcgtcgcggc
    10261 attgaccagt ggcaaccact catcaccagt gataatggct gcgggctggc aacttgccag
    10321 taaaccctgc agtttggcgc tccaggaatc ccgctgacct acgcccattg gaatcgccaa
    10381 cgggacggcg actaagccgg catactggca ggcaaaaaaa gcctctacaa acccgctact
    10441 cgtttcggca atcagcgcca cgcgatcgcc ttttttcagg ttcagcgata acaacctttt
    10501 cgcaccagct tcggcacgcg cttttaacgt ctgatattcc agttgatctt caagttggca
    10561 acgtctgtca taaaaattca ttccggcgct actcagagcg gcgtagtcca aagcatcaac
    10621 cagcgttgga aaatcggcat agcgcatagg tagggaatgc gtaaa
//
LOCUS       DAZFJL010000157        10449 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZFJL010000157 DAZFJL010000000
VERSION     DAZFJL010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.159"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            122..2263
                     /locus_tag="ACHJ8C_004050"
     CDS             122..2263
                     /locus_tag="ACHJ8C_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFO5553828.1"
                     /translation="MAMFTPSFSGLKGRALFSLLFAAPMIHATDSVTTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHATRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESVGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(2327..4219)
                     /gene="parE"
                     /locus_tag="ACHJ8C_004051"
     CDS             complement(2327..4219)
                     /gene="parE"
                     /locus_tag="ACHJ8C_004051"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO5553829.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(4248..4829)
                     /gene="yqiA"
                     /locus_tag="ACHJ8C_004052"
     CDS             complement(4248..4829)
                     /gene="yqiA"
                     /locus_tag="ACHJ8C_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO5553830.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQMGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(4829..5656)
                     /gene="cpdA"
                     /locus_tag="ACHJ8C_004053"
     CDS             complement(4829..5656)
                     /gene="cpdA"
                     /locus_tag="ACHJ8C_004053"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO5553831.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADARFQPDTASEGY"
     gene            complement(5681..6103)
                     /locus_tag="ACHJ8C_004054"
     CDS             complement(5681..6103)
                     /locus_tag="ACHJ8C_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO5553832.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(6104..6733)
                     /gene="nudF"
                     /locus_tag="ACHJ8C_004055"
     CDS             complement(6104..6733)
                     /gene="nudF"
                     /locus_tag="ACHJ8C_004055"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO5553833.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVVRREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6938..8419
                     /gene="tolC"
                     /locus_tag="ACHJ8C_004056"
     CDS             6938..8419
                     /gene="tolC"
                     /locus_tag="ACHJ8C_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO5553834.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNMLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            8567..9238
                     /locus_tag="ACHJ8C_004057"
     CDS             8567..9238
                     /locus_tag="ACHJ8C_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO5553835.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            9244..10404
                     /locus_tag="ACHJ8C_004058"
     CDS             9244..10404
                     /locus_tag="ACHJ8C_004058"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO5553836.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
BASE COUNT         2634 a         2772 c         2585 g         2458 t
ORIGIN      
        1 gtgattgaat gattctcaat tacatatccg atggcgaaat aacaaacgga tagtgcttag
       61 tgagcaataa gaaacaattt tcactcgcga caatccgcga gtttttggga taacagacta
      121 aatggctatg ttcacacctt cattctcagg actcaaaggt cgggcgctct tttcactgct
      181 ttttgcggca ccgatgattc atgcaacaga ctctgtaacg accaaagatg gcgaaacaat
      241 cactgttaca gcagatgcaa ataccgcaac tgaggcaacc gatggttatc aacctctgag
      301 cacctccacg gcgacattaa ccgatatgcc gatgctggat atcccgcagg tggtcaatac
      361 ggttagcgat caggttctgg aaaaccagaa tgcgacaacg ctggatgagg cgctttataa
      421 cgtcagtaac gtggtacaga ccaatacatt aggcgggact caggatgctt ttgtacgccg
      481 tgggtttggc gcaaaccggg atggctccat catgaccaac ggtctgcgaa ccgtacttcc
      541 tcgcagtttc aacgccgcca cagaacgtgt agaagtgcta aaaggcccgg cctccacgct
      601 gtatggcatt ctcgatcctg gcggactgat taacgtcgtg accaagcgcc cggaaaaaac
      661 attccatggt tcggtttcag ccacctcctc cagttttggt ggcggcactg ggcaacttga
      721 tatcacaggt cccattgaag gcactcagct ggcgtatcgc cttaccgggg aagtgcagga
      781 tgaagattac tggcgaaact tcggtaaaga gcgcagtaca tttattgccc cgtcactcac
      841 ctggtttggt gacaatgcaa cagtaaccat gctctattcc catcgggact ataaaactcc
      901 gttcgatcgt ggaacgattt tcgaccttac gacgaaacag cccgtaaacg ttgatcgaaa
      961 aatacgtttt gacgaaccgt ttaatattac agatggtcag tccgatctgg cgcaactcaa
     1021 cgcagaatat catctcaata gccagtggac agcgcgcttt gattacagct acagccagga
     1081 taaatacagc gataatcagg cgcgtgttac cgcgtatgat gcaacgacag gaacactgac
     1141 acggcgtgtt gatgcaactc agggatctac ccagcgtatg catgctactc gtgcggatct
     1201 gcaagggaat gttgatattg ccggattcta taatgagatt ctgggtgggg tgtcatatga
     1261 atattatgat cttctgcgta cagatatgat tcgctgtaaa aaagctaaag atttcaatat
     1321 ctacaaccct gtttatggta ataccagcaa atgtacaacg gtttcggcat cggacagtga
     1381 tcagacgatc aaacaggaga gctactcagc gtatgcacag gacgcactct atctgaccga
     1441 taactggatt gccgtcgccg ggatccgcta tcagtattac acgcagtatg cgggtaaagg
     1501 ccgtcctttt aatgtcaata ctgacagccg cgatgaacaa tggactccca aactggggtt
     1561 ggtctacaaa ctgacgccat cggtatcctt atttgccaat tattcgcaaa catttatgcc
     1621 acagtcgtca attgccagct acattggcga tcttccacca gaatcatcta atgcttacga
     1681 agtcggggca aaattcgagc tattcgatgg tatcaccgca gatattgcgc tgtttgatat
     1741 ccataaacgt aatgtgttgt ataccgaaag tgttggtgat gaaaccattg ccaaaacggc
     1801 aggccgtgtt cgttcaagag gggtagaagt cgaccttgcg ggagcattaa ctgaaaacat
     1861 taatatcatt gccagctacg gctataccga tgctaaggtt ctggaagatc ctgattatgc
     1921 aggtaaacca ttgccgaatg ttcctcgtca taccggttcg ctattcctga cctatgacat
     1981 tcataacatg cctggcaata acacactgac gtttggcggt ggtggacatg gcgtaagccg
     2041 tcgttcggca accaatgggg ctgactatta tctgccaggc tattttgttg ccgatgcctt
     2101 cgccgcatac aaaatgaaat tgcagtatcc ggtcacactg caattaaacg tcaaaaatct
     2161 gtttgataaa acgtattaca cctcttccat cgccacaaat aatctgggca accagattgg
     2221 cgatccgcgt gaagtgcaat tcacggtgaa aatggaattt tgatcaaaaa agcccggtat
     2281 acacttgtat accgggctat gttttattct tctggcggga ggattatcag acctcaatct
     2341 ctgccatgtc gcctttctct tgcaaccaat tgcggcgatc ttccgagcgt ttcttcgcca
     2401 gcagcatatc catcatcgcg tcagtacgct gatcgtcttc atcatcgata gtcaactgca
     2461 ccagacggcg agtgttcgga tcaagcgtag tttcgcgcaa ttgcatcggg ttcatttccc
     2521 cgagaccttt aaaacgctgg acgtttggct tgcctttctt gcgttttaat tgctcaagta
     2581 cgccctcttt ctcttcttcc gtcagcgcgt aataaacctc tttcccgagg tcaatacggt
     2641 agagcggtgg cagtgcaacg taaacgtgac cgtgtttcac caatgcgcgg aagtgtttta
     2701 caaacaaagc gcagagcagc gtggcaatgt gcagaccatc ggagtcagca tccgccagga
     2761 tacagatctt gccgtaacga agctggctca aatcgtcgct gtcaggatcg ataccgatag
     2821 ctaccgaaat atcgtgcact tcctgcgaag ccagcacttc gtcggaagag acttcccagg
     2881 tgttaaggat cttacctttc agtggcatga tcgcctgata ttcgcgatcg cgcgcctgct
     2941 tggcagatcc gcctgcggag tcaccttcca caaggaatag ttcggtacgg ttaagatcct
     3001 gcgcggtaca gtcagccaat ttaccaggca gcgccgggcc gctggttagc tttttacgca
     3061 ccactttttt agccgcacgc atacgacgct gggcgctgga aatcgccatc tctgccaaca
     3121 gctccgccgc ctgaacgttt tggttcagcc acaggatgaa ggcatctttc accacgcccg
     3181 aaacgaatgc cgcacactga cgcgaagaga gacgttcttt ggtctgcccg gcaaactgcg
     3241 gatcctgcat ttttactgac agcacatagg cgcagcgatc ccagatatct tccgccgaca
     3301 gctttacacc acgcggcaga atgttgcggt attcacagaa ctcacgcatc gcgtccagca
     3361 ggccctgacg caggccatta acatgggtac cgccctgcat cgttgggatc aggttgacgt
     3421 agctttcggt cagcagttca ccgccttccg gcagccacag tagcgcccag tccaccgctt
     3481 cagtatcgcc agcgaaatta ccgataaacg gtttttctgg cagcgtcggt aaaccgttta
     3541 ccgcttccgc caggtaatca ttcagaccgt cctgatagca ccagcgctgt tcggtgttgt
     3601 tgatctcatc tttaaaagtg atctcaacgc ccgggcacag taccgctttg gctttcagca
     3661 catgcgtcag gcgtgaaaca gaaaaacgcg ggctgtcaaa gaaggtttca tccggccaga
     3721 agtgcacgct ggtaccggta ttgcgtttac cgcaagtgcc gacaacctgt aaatcttgca
     3781 ccttttcgcc attttcaaag gcgatgttat agatctgacc atcgcggcgt acgttaactt
     3841 ctacgcgctt cgacagggcg ttaactaccg aaatccccac gccatgcagg ccgccagaga
     3901 actggtaatt tttgttagag aatttaccgc ccgcgtgcag acggcaaaga atcagttcaa
     3961 ccgccggtac cccctcttcc gggtgaatat ctaccggcat cccgcgccca tcgtcaatca
     4021 cttctaacga ctggtcggca tgtaagatta catccacgcg ttttgcgtgg cccgccagtg
     4081 cttcatcgac actgttatca ataacttctt gccccaaatg gttagggcga gtggtatcgg
     4141 tatacatccc cggacggcgg cgaaccggct caagcccggt gagtacctca atggcatcag
     4201 cgttataagt ttgcgtcatg gtttaagtta gcaattcgag ttgatcgtca gagatggtgc
     4261 agaccaagaa aatcgacgat cgggttgaaa taatcttcga agcccgtgaa tgcgtggttg
     4321 ccgccttcta tgacagtctg gcggcaggaa gcgtagtacg ccaccgcctg gcggtaatcc
     4381 agcacttcat ctcccatctg ttgcagcagc cagatcaaat ccggcgcttc cagcgggtca
     4441 atctgcatga ctttgagatc gtaaatatgg cgtgactcta gcacatattg ctgcccggtg
     4501 taggggttct cgttctgacc gagatagtcc gtcagcagtt caaacgggcg caccgccggg
     4561 ttgaccacca ctgcgggcag cataaaacat tgcgacaacc aggtggcgta atatcccccc
     4621 agtgacgaac cgacaatacc cagcgaatcg ccgccatgtt ccaggacaat ggattccagc
     4681 agttctgccg cgtcagaagg atacggcggc aactgcggaa tgatcatctc aacgtcaggg
     4741 tgatgttccg ccagccagtt ttttaacaag ctcgcttttg cagagcgcgg cgagctgttg
     4801 aaaccgtgta aataaagaag cgtagacatc agtagccttc tgaagcggta tcaggttgga
     4861 agcgtgcgtc cgccaggcga tgcacctcgg tggtcagcgt gccatcagca tgtaactcga
     4921 gagtacgcca gccgggcgcg atggtgtcca gcgtaaagtt agaacaatgc ggcttaaact
     4981 gcacacaggt cgacggcgtt gccagcaagc ggcgaccgtt ccagtcgaga tccagctcct
     5041 gatgaatgtg accgcacagc aagtatttga cgtgcggaaa cttcgccagc acggtatcca
     5101 gttcgcccgc gttacgcagg ctgtgttgat caagccaact acaacctgca ggtagcggat
     5161 gatgatgcag cagcagcaac gtatggcgtt ctggcgcatc ggccagttta cgttccagcc
     5221 actcaagctg aaactcgctc agctcaccgt gcggcacgcc aaacacctgg ctatccagca
     5281 acaggatttg ccattgctca ccaataaaca cgcgcttcgc cggggagata cccgcatcct
     5341 gtaacgcact gtacatcgcg ggctggaaat cgtggttgcc cggcagccag acgcagggcg
     5401 cacgaaaact tgcgatgcct tcagcgaaat gctgataggc cgcagaggat tgatcctgcg
     5461 ctaaatcacc tgtcgcgaca atcaggtcga attcgtgctg gtgtggccga atcgcctcca
     5521 gcaccgcctg gtagctttcc caggtgttta cccctaacag ggcttcgtgc ttttgtgcaa
     5581 acaggtgagt gtcggtaatt tgtaaaatcc tgactctggc ctcaccagcc agaggaaggg
     5641 ttaacaggct ttccaaatgg tgtccttagg tttcacgacg ctaataaacc ggaatcgcca
     5701 tcgctccatg tgctaaacag tatcgcaacc agtccgctaa aaactgatta atttgatgct
     5761 tttcgtcgcg ttgatgcaac tttttattag gataatcata ccgcgctttg aagcgaaaaa
     5821 tctgctggct tgaacacact tcagccacca tcgcgtcatg atacagacgc accgtcattg
     5881 acggaaggct ccagtaactg atcgcgggcg cagtctgttc tattgtcacc agggtagtgt
     5941 atcgggtcga ttccacaatc gtcagccgat attgtgcgtt tgccacctga tagcttacag
     6001 tttcgccggg tgcgtcattg cgcggtaaca aacggcgcaa ttgtgaaaag ttcatctcgc
     6061 acaggcgcat catttcagga aagtcaggtg tgtaacgctt catttatgcc cactcatttt
     6121 ttaacgcttg atgatgcagc tgcagccatt gcaaagcgat gaccgacgct gcgttgtcga
     6181 ttttcccctc ttctacccac tggtatgcct gttcccggct taccacatga acgcgaatat
     6241 cttcgttttc atcagccaga ccgtgaatac cgcttgcggt cgtggcgtcc acttcgccca
     6301 ccataattga cgaacgctca ctggtgcccc ccgggcttgc caggaaactt aacaccggtt
     6361 tggtccgttt gactatcagt cccgcctctt caatcgcttc gcgacggaca acatcttcca
     6421 cactttcacc ctcttcaatc atcccggcaa ccatctccag tagccagggg gtttcgctgg
     6481 tgtcgtacgc ggcaatccga atctgctcaa tcagcacaac ttcatcacgt actgggtcaa
     6541 agggtagcaa gactgcggcg tgaccgcgct caaaaatttc ccgccgtacc tcatgactca
     6601 tttgcccgtt gaatagacga tgacgaaatc tataaagatc taatgaaaaa aagccgcgat
     6661 aaagtgtttc tcgtgcaata atttctacat cgtttttgcc aaatgtaacg ggcaggttgt
     6721 ctggcttaag cattgttaat gtcctggcac taatagtgaa ttaaatgtga atttcagcga
     6781 cgtttgactg ccgtttgagc agtcatgtgt taaattgagg cacattaacg ccctatggca
     6841 cgtaacgcca accttttgcg gtagcggctt ctgctagaat ccgcaataat tttacagttt
     6901 gatcgcgcta aatactgctt caccacaagg aatgcaaatg aagaaattgc tccccattct
     6961 tatcggcctg agcctttctg gtttcagttc gttgagccag gccgagaacc tgatgcaagt
     7021 ttatcagcaa gcacgcctta gtaacccgga attgcgtaag tctgccgccg atcgtgatgc
     7081 tgcctttgaa aaaattaatg aagcgcgcag tccattactg ccacaactag gtttaggtgc
     7141 agattacacc tatagcaacg gctaccgcga cgcgaacggc atcaactcta acgcgaccag
     7201 tgcgtccctg cagttaactc aatccatttt tgatatgtcg aaatggcgtg cgttaacgct
     7261 gcaggaaaaa gcagctggga ttcaggacgt cacgtatcag accgatcaac aaaccttgat
     7321 cctcaacacc gcgaccgctt atttcaacgt gttgaatgcg attgacgttc tttcctatac
     7381 acaggcgcaa aaagaagcga tctaccgtca attagatcaa accacccaac gttttaacgt
     7441 gggcctggta gcgatcaccg acgtgcagaa cgcccgcgca cagtacgata ccgtgctggc
     7501 gaacgaagtg accgcacgta ataaccttga taacgcggta gagcagctgc gccagatcac
     7561 cggtaactac tatccggaac tggcggcgct gaatgtcgaa aactttaaaa ccgacaaacc
     7621 acagccggtt aacatgctgc tgaaagaagc cgaaaaacgc aacctgtcgt tgttacaggc
     7681 gcgcttgagc caggacctgg cgcgcgagca aattcgccag gcgcaggatg gtcacttacc
     7741 gactctggat ttaacggctt ctaccgggat ttctgacacc tcttatagcg gttcgaaaac
     7801 ccgtggtgcc gctggtaccc agtatgacga tagcaatatg ggccagaaca aagttggcct
     7861 gagcttctcg ctgccgattt atcagggcgg aatggttaac tcgcaggtga aacaggccca
     7921 gtacaacttt gttggtgcca gcgagcaact ggaaagcgcg catcgtagcg tcgtgcaaac
     7981 cgtacgttcc tccttcaaca acattaatgc ttctatcagt agcattaacg cctacaaaca
     8041 agccgtagtt tccgctcaaa gttcattaga cgcgatggaa gcgggttact cggtcggtac
     8101 gcgtaccatt gttgatgtgt tggatgcgac caccacgctg tacaacgcta agcaagagct
     8161 ggcaaatgcg cgttataact acctgattaa ccagttgaat attaagtcag ctctgggtac
     8221 gttgaacgag caggatctgc tggcactgaa caatgcgctg agcaaaccgg tttccactaa
     8281 tccggaaaac gttgccccgc aaacgccgga acagaatgct attgctgatg gttatgcgcc
     8341 tgatagcccg gcaccagtcg ttcagcaaac atccgcacgc actaccacca gtaacggtca
     8401 taaccctttc cgtaactgac gacgacgacg gggcttcggc cccgtctgaa cgtaaggcaa
     8461 cgtaaagata cgggttatct gccgcattct tcccccttct cgcttcaatt tcgaccagcc
     8521 atcctctatt ctgatgggta tttaccactg gtcccggaag acaaaaatga aacggacaaa
     8581 atccatacgc cacgcatcgt tccgcaaaaa ctggagcgca cgccatctga caccagtcgc
     8641 tctcgcggtt gccactgttt ttatgctggc gggctgtgaa aagagtgatg aaacagtgtc
     8701 tctctatcaa aatgctgacg actgttcagc tgcaaaccca ggcaaaagcg ccgaatgtac
     8761 caccgcgtac aacaatgcgc tgaaagaagc cgaacgtact gcgccgaaat acgccacccg
     8821 tgaagactgt gttgctgaat ttggtgaagg tcagtgccag caggcaccag cccaggctgg
     8881 catggcacca gaaaaccagg cgcaggccca gcaatccagc gggagtttct ggatgccgct
     8941 gatggccggt tacatgatgg gacgtctgat gggcggcggc gcgggatttg cacagcagcc
     9001 gctgttctcc tcgaaaaacc cggccagccc ggcttacggt aaatataccg acgcgaccgg
     9061 taaaaactat ggcgcagccc agccaggccg caccatgacc gtaccgaaga cggcaatggc
     9121 accaaaaccg gcgaccacta ctactgttac ccgtggcggt tttggtgaat ctgttgccaa
     9181 acaaagcact atgcagcgta gcgcaaccgg cacctcttct cgttcaatgg gtggctgata
     9241 ccgatggaaa gagtcagtat taccgagcgc ccggactggc gcgaaaaagc ccacgaatac
     9301 ggtttcaatt ttcacaccat gtacggcgag ccgtactggt gtgaagatgc ttactacaag
     9361 ttgaccctcg cccaggttga aaagctggaa gaagtcaccg ccgaactgca ccagatgtgc
     9421 ctgaaagtgg tggaaaaagt gatcgccagc gatgagctga tgaccaaatt ccgcattcca
     9481 aaacacacct ggagttttgt gcgccagtca tggctgacgc accagccatc gctttattcg
     9541 cgtcttgatc tggcgtggga tggcactggc gaacctaaac ttctggaaaa taacgccgat
     9601 acgccaacgt cactatacga ggcggcgttc tttcagtgga tctggctgga agatcagctt
     9661 aacgccggta acttgccgga gggcagcgac cagtttaaca gtttgcaaga aaagctgatc
     9721 gatcgcttcg ttgagctgcg tgaacagtat ggcttccagt tgctgcatct cacctgctgt
     9781 cgcgacacgg tggaagatcg cggaaccatt cagtatttgc aggactgcgc gacggaagct
     9841 gaaatcgcta ccgagttcct ctacatcgac gatatcgggt taggtgaaaa aggtcagttc
     9901 acggatttac aggatcaggt gatttccaac ctgttcaagc tgtatccgtg ggaatttatg
     9961 ttgcgtgaga tgttctccac caagctggag gatgcaggcg tacgctggct ggaaccggcg
    10021 tggaagagca ttatctccaa caaagcgctt ctaccgctac tgtgggagat gttcccgaat
    10081 cacccgaacc tgctgcccgc ttattttgcg gaagatgatc atccgcaaat ggaaaaatat
    10141 gtggttaaac cgatcttctc ccgtgaaggc gcaaacgtgt cgatcattga gaacggcaaa
    10201 accattgaag cagcggaagg tccgtatggc gaagaaggga tgattgttca gcaattccac
    10261 ccgttaccga aattcggcga cagctatatg ctgattggta gctggctggt gaacgatcaa
    10321 cctgccggaa ttggcattcg tgaagaccgt gcattgatca cccaggatat gtctcggttt
    10381 tatccgcata tttttgttga gtaatacagc ttaatattgc cccgataagc ggggcaatct
    10441 cttataatc
//
LOCUS       DAZFJL010000158        10166 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZFJL010000158 DAZFJL010000000
VERSION     DAZFJL010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.160"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            224..1597
                     /gene="aroP"
                     /locus_tag="ACHJ8C_004059"
     CDS             224..1597
                     /gene="aroP"
                     /locus_tag="ACHJ8C_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO5553837.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYRYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGAWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1640..2494)
                     /gene="ampE"
                     /locus_tag="ACHJ8C_004060"
     CDS             complement(1640..2494)
                     /gene="ampE"
                     /locus_tag="ACHJ8C_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO5553838.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDGVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2491..3042)
                     /gene="ampD"
                     /locus_tag="ACHJ8C_004061"
     CDS             complement(2491..3042)
                     /gene="ampD"
                     /locus_tag="ACHJ8C_004061"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO5553839.1"
                     /translation="MLLEQGWLAGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAYPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3130..4023
                     /gene="nadC"
                     /locus_tag="ACHJ8C_004062"
     CDS             3130..4023
                     /gene="nadC"
                     /locus_tag="ACHJ8C_004062"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO5553840.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4227..4667
                     /gene="ppdD"
                     /locus_tag="ACHJ8C_004063"
     CDS             4227..4667
                     /gene="ppdD"
                     /locus_tag="ACHJ8C_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO5553841.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4677..6062
                     /gene="gspE"
                     /locus_tag="ACHJ8C_004064"
     CDS             4677..6062
                     /gene="gspE"
                     /locus_tag="ACHJ8C_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO5553842.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLACRGGEKVVLRLLQQVNQALDVNTLGMQPSQLVDFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQMLNTADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6052..7254
                     /gene="hofC"
                     /locus_tag="ACHJ8C_004065"
     CDS             6052..7254
                     /gene="hofC"
                     /locus_tag="ACHJ8C_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO5553843.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGVAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLASFSGEWGW
                     LLVLFGFLLAIANKLLMRHPTRLIARQKLLLRIPIMGSLMRGQKLTQIFTILTLTQSA
                     GISFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGIG"
     gene            complement(7289..8332)
                     /gene="guaC"
                     /locus_tag="ACHJ8C_004066"
     CDS             complement(7289..8332)
                     /gene="guaC"
                     /locus_tag="ACHJ8C_004066"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO5553844.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8488..8532)
                     /locus_tag="ACHJ8C_004067"
     CDS             complement(8488..8532)
                     /locus_tag="ACHJ8C_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553845.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8557..9177
                     /gene="coaE"
                     /locus_tag="ACHJ8C_004068"
     CDS             8557..9177
                     /gene="coaE"
                     /locus_tag="ACHJ8C_004068"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO5553846.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANQEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9177..9920
                     /gene="zapD"
                     /locus_tag="ACHJ8C_004069"
     CDS             9177..9920
                     /gene="zapD"
                     /locus_tag="ACHJ8C_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO5553847.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            9930..10127
                     /gene="yacG"
                     /locus_tag="ACHJ8C_004070"
     CDS             9930..10127
                     /gene="yacG"
                     /locus_tag="ACHJ8C_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO5553848.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSSDLSESDDWSEEPKQ"
BASE COUNT         2504 a         2683 c         2594 g         2385 t
ORIGIN      
        1 gaggctgacc tttacaaacg gtttcttttt aagcaactca tcttcaacca tgcataaagc
       61 gggtgcattc gctgccgcat accattattc ttgatctgac ggaagtcctt ttgtaacaat
      121 tcaaacttcc ttgatgtaaa caaatcaata caacaaacgg aattgcaaac ttacacacgc
      181 atcactgcgt agatcaaaaa aaacaatcac cgcacgaggt ttcatgatgg aaggtcaaca
      241 gcacggcgag cagctaaagc gcggccttaa aaaccgccat attcagctta tcgcgctggg
      301 tggcgcgata gggacagggc tattcctggg tagcgcctcc gtaatacagt ccgcagggcc
      361 agggattatc ctgggttacg ccattgctgg ttttatcgcc tttctgatca tgcgtcagct
      421 aggtgaaatg gtggtcgaag aacctgtcgc aggctccttt agccactttg cttatagata
      481 ctggggcagt tttgccggtt tcgcctctgg ctggaactac tgggtactgt acgttttagt
      541 tgccatggca gagctgactg ccgtgggtaa atacattcag ttctggtatc cggaaatccc
      601 aacctgggtt tctgccgccg tgttctttgt ggtgatcaac gccatcaacc tgacaaacgt
      661 aaaagtgttt ggcgagatgg agttctggtt tgccattatc aaagttatcg cggtggtagc
      721 gatgatcatc ttcggcgcct ggctgctatt cagcggcaac ggcggcccgc aggcgagcgt
      781 tagcaacctg tgggatcagg gtggtttcct gccacacggc ttcaccggtc tggtgatgat
      841 gatggcgatt atcatgttct ctttcggtgg tctggaactg gtggggatca ccgcagcaga
      901 agctgataac ccggagcaaa gtatcccaaa agcaactaac caggttatct accgcatcct
      961 gattttctat attggttcgt tagccgttct gctctcacta atgccgtgga cccgcgttac
     1021 cgccgatacc agtccgtttg tgctgatctt ccacgagtta ggcgatacct ttgtagcgaa
     1081 tgcgctgaat atcgtggtac tgactgctgc gctctccgtg tacaacagct gcgtatattg
     1141 caacagccgt atgctgttcg gtctggcaca acaagggaac gcgccaaaag cgctggcgtc
     1201 tgtcgataaa cgcggcgtac cggttaacac tattctggtg tctgcgctgg ttacggcatt
     1261 gtgcgtactg attaactacc ttgccccaga gtccgctttc ggactgttaa tggcgctggt
     1321 ggtatctgca ctggtcatca actgggcgat gatcagtctg gcgcatatga aattccgtcg
     1381 tgccaaacaa gaacaaggcg tggtaactcg cttccctgcc ctgctttatc cgctgggtaa
     1441 ctggatctgc ctgctgttta tggcggcggt actggtgatt atgctgatga ccccaggaat
     1501 ggcgatttcg gtatacctga tcccagtatg gctgatcgtg ttaggtatcg gctatctgtt
     1561 taaagagaaa accgccaaag ccgtaaaagc gcattaatct ctctacgccc tcacccgtgc
     1621 agggtgaggg caataatctt tacaccaacg ccccgtaaat cgtcagtagc gcaattacca
     1681 ccacgaccac gaacgaggtt ttcttcgcca ttgaaaccgc tgcctttggc gtctccacct
     1741 tatcgacatg cggttcacgc gccagagaga actgtgccag acgcgttaat acctgatatt
     1801 gcgaagtatg gaaatcaccc agcgaagcaa accaggccgg taacgctttc tcaccatgac
     1861 cgatcaaggc atataccaca cccgcaagac gaaccggcac ccaatccagt acatgaagca
     1921 cgccatcaat gccggactgt aaacgatgat gcggcgtctg gtatcgcgcc agccagtatt
     1981 gccatgcacg caaaaacgca taccccatca gcgtaacggg tccccaggtt ccccccacaa
     2041 tcagccagaa cagcggagca agataaaaac gaaagttaat ccacagcaat gcattttgca
     2101 gctcacgcaa atactcacgt tcatcgcagc ctgccgggac gccgtgaatc atggtgagtt
     2161 cgccagccat cgtggcacgg gcatggctat cattacgtga agcagctgtc agataagcat
     2221 gataatgaag acgaacttta cctgcgccaa tacacagcaa accaatcagc agccacacca
     2281 gcagcgtggg aacgttgaac aatactccct gcaatgcgcg taacagtaaa aaagtcacgc
     2341 ccatcgcaat aatggtcatg cctaatgtgc gcccgagaga aaaatgtttc acccgccgaa
     2401 agaacgcttc aagacgatga tcaagctgcc agtgctcgcc caacttaaac aggcgctcga
     2461 aaattaacac cagtaaggtt gtaaatagcg tcatgttgtc tccttgctga ccagcgcacg
     2521 aaaccgtgcc caatcaaatg cagggccggg atcggttttc cgatccggcg caatatcaca
     2581 atggcccgtc atgttattag cgatatccgg atagcgatca atcagtgccc gcgtaaccgc
     2641 cgcaagctgt tgatactgcg catcggtata cgccagcgta tcggtgcctt ccagctcaat
     2701 cccaatagaa aaatcattgc agcgttcacg cccctgatac tgagagactc ccgcatgcca
     2761 ggcacgttta tcgaaaggaa catactggac tatttcacca tcgcggcgaa tcaaacagtg
     2821 agcggagacg cgcaaatggg cgatctcagc aaagaaagga tacgcctgcg gatcaatagt
     2881 tccagtgaat aatgcgtcga tccacggacc gccaaactcg cctggcggca ggctaatatt
     2941 gtgcaccacc agcagagtgg gtgtttcgtc atccgggcgg caatcgtaat gtggtgaggg
     3001 aacgcggcgc gcgccagcca gccacccctg ttctaacaac atgcaggatc tccttatatg
     3061 tggtgctaat acccggttca gagtagcatg tttctacctt atgattcgtt agctatctgg
     3121 agttttaaca tgccgcctcg ccgctataac cctgacaccc gacgtgacga gctgctggaa
     3181 cgcattaatc tcgatatccc cggcgcggtg gcccaggcgc tgcgggaaga tttaggcgga
     3241 acagtcgatg ccaacaatga cattacggca aaacttttac cggaaaactc tcgctctcat
     3301 gccacagtga tcacccgcga gaatggcgta ttttgcggca aacgctgggt tgaagaggtg
     3361 tttattcaac tggcaggcga cgatgtcacc attatctggc atgtggatga cggcgatgtc
     3421 atcaacgcca atcaaccctt gttcgaactt gaaggcccat cccgcgtgct gttaacgggc
     3481 gaacgcactg cgcttaattt tgtgcaaact ctttcaggag ttgccagtaa ggtacgccac
     3541 tatgtcgaat tactggaagg caccaacacg cagctgttgg atacgcgtaa aaccttgccc
     3601 ggcctgcgtt cagctctgaa atacgcggta ctttgcggcg gcggagcgaa tcaccgcctg
     3661 gggctttctg atgccttcct gatcaaagaa aaccatatta ttgcctccgg ctcagtgcgg
     3721 caggcggtcg aaaaagcgtc ctggttgcac ccggatgcgc cagtagaagt cgaagtagag
     3781 aatctggaag aacttgatga agctctgaaa gcaggtgccg atatcatcat gctggataac
     3841 ttcgaaacag aacagatgcg cgaagccgtc aaacgcacca acggcaaggc gctactggaa
     3901 gtgtctggca acgtcactga caaaacactg cgcgaatttg ccgaaacggg cgtggacttt
     3961 atctccgttg gtgcgctaac taaacacgta caggcactcg acctttcaat gcgttttcgc
     4021 taattgctaa cagatttatc cgctccatcg cgggcggata aatctcccct tccccaactt
     4081 tcttcgtaac acctcgcaaa tttcttgtct ttcgctgcaa cgaagtaaaa aacctcggaa
     4141 gctgctttcc cgtttcgttt tttgcccctc gcttgtgctg atggcgtttg ccaaagtagc
     4201 gccaaccaaa tcaaggagcg aaacagatgg acaagcaacg cggttttaca cttatcgaac
     4261 tgatggtggt tattggcatc attgccattt taagcgccat tggtattccc gcttatcaaa
     4321 actacctgcg caaagccgca ctcaccgaca tgctacaaac ctttgtgcct taccgtaccg
     4381 ccgtagagtt gtgcgcgctg gaacatggtg gattaaatac ctgcgacggt ggcagcaatg
     4441 gcattccctc gcctaccacc acccgctatg tttcagccat gagtgtggca aagggcgtgg
     4501 tgtcgctgac cgggcaagaa agtctcaatg gactaagcgt cgtcatgacg ccgggttggg
     4561 ataacgcaaa cggcgtcacc ggctggacgc gtaactgcaa tattcaaagt gacagcgcat
     4621 tacagcaagc ctgcgaagat gtcttccgct ttgatgacgc caactaagga gcggcaatga
     4681 atattccaca gctcactgcc ctgtgtctgc gttatcaggg agtcttgctg gatgccagcg
     4741 aagaggtggt tcatgttgcg gtagtcgatg caccttcgca tgagctactg gacgcattgc
     4801 atttcgctac caccaaacgt attgagatca cctgctggac gcgccaacaa atggaaggtc
     4861 acgccagtcg cacacaacag acattgcccg tagctgttca ggagaagcat cagcccaaag
     4921 cagagttgct gactcgaacg ttacaatctg cgctggaaca acgcgcgtct gatattcata
     4981 tcgaaccagc ggacaatgcc taccgcatcc gcttgcgtat cgacggcgta ttgcatcctt
     5041 taccggacgt ttcaccggat gccggagtcg cattaaccgc cagattaaaa gtgctgggaa
     5101 acctggatat tgcggaacat cgcctgccgc aggacgggca attcactgtc gaactggcag
     5161 gaaacgccgt ctcatttcgt attgcgacct tagcatgtcg gggtggtgaa aaggtggtat
     5221 taaggttgtt acagcaggtg aaccaggcac tggatgttaa cacgcttgga atgcagccgt
     5281 cacaactggt ggactttgct catgccttgc aacaaccaca gggactggtg ctggtaactg
     5341 gccctactgg cagcggcaaa acggtcacgc tttatagtgc cctgcaaatg ctgaataccg
     5401 ctgacattaa tatttgtagc gtcgaagatc cggttgagat ccccatagcc ggactaaacc
     5461 agacgcaaat ccattcgcgt gccggactca cctttcaggg cgttttgcgt gcgttattgc
     5521 gccaggatcc tgacgtcatc atgatcggag agatccgcga tggcgaaacg gcagaaattg
     5581 ccattaaagc cgcgcaaacc ggtcacctgg tgttgtctac cctacacact aactccacct
     5641 gcgaaacgct ggtacgttta cagcaaatgg gagtcgcccg ctggatgcta tcatcggcgc
     5701 ttacgctggt aatagcccag cgtctggtac gcaaactttg cccgcattgt cgccggcagc
     5761 aaggggagcc catccatatt ccagacaatg tatggccatc gccgctgccc cactggcagg
     5821 cacccggttg tgtacattgc taccacggtt tttatggtcg cacggcctta tttgaagttc
     5881 tgcccataac accggtcata cgtcagctta tttccgctaa taccgacgtt gaatcgctgg
     5941 aaacgcacgc ccgacaggcg ggtatgcgaa cgctttttga aaacggctgc ctggccgtgg
     6001 agcaaggctt aaccaccttt gaagagttaa tccgcgtatt ggggatgccg catggcgagt
     6061 aagcaactct ggcgctggca tggcataacc ggcgacggca atgcgcaaga tgggatgcta
     6121 tgggcagaga gccgtgcttt gctgctcatg gcactacagc aacagatggt taccccacta
     6181 agcctgaagc gaatcgccat caattctgcg cagtggcgag gagataaaag cgcggaagtc
     6241 attcatcaac tggcgacgct actcaaagcc gggttaacgc tttctgaagg gctggctctg
     6301 ctggcggaac agcatcccag taagcagtgg caagcgttgc tgcaatcact ggcgcacgat
     6361 cttgaacagg gcgttgcctt ttccaatgcc ttattaccct ggtcagaggt atttccgccg
     6421 ctctatcagg cgatgatccg cacgggtgaa ctgaccggta agctggatga atgctgcttt
     6481 gaactggcgc gtcagcaaaa agcccagcgt cagttgaccg acaaagtgaa atcagcgtta
     6541 cgttatccca ttatcatttt agcgatggca atcatggtgg ttgtggcaat gctgcatttt
     6601 gtactgccgg agtttgccgc tatctataag acctttaaca ctccgctacc ggcgctaacg
     6661 caggggatca tgacgctggc aagttttagt ggcgaatggg gttggttgct ggtgctattc
     6721 ggctttctgc ttgcgatagc caataagctg ctgatgcgcc acccaaccag gcttattgca
     6781 cggcagaaat tgctgttacg catcccgatt atgggttcac tgatgcgggg acaaaaactc
     6841 acgcagatct ttacgattct gacactgaca caaagtgcag gcatttcttt tttacagggc
     6901 gttgagagcg tcagagaaac aatgcgctgc ccgtactggg tgcaacttct gacacaaatc
     6961 cagcacgata tcagtaacgg tcaccccatc tggctggcgc taaaaaatgc cggtgagttt
     7021 agcccgctct gtttgcaatt agtgagaaca ggagaggcat ccggctcgct ggatctcatg
     7081 ttagacaacc tcgcccatca tcatcgggat aacacaatgg cgctggcgga taacctcgca
     7141 gccttactgg aaccggcgtt gctgatcata acgggaggaa ttatcggtac gctggtggtg
     7201 gcaatgtatc tgccaatttt ccatttaggc gatgcgatga gtggaatagg ataacgctgg
     7261 cgtgttgctc cacgccagcg ttggtatatt acaggttgtt gaagatgcgg ttttcttgtt
     7321 cctgcacacg gataaacgtg gtgcgcttgg tcagttcttt caggcgtgaa gccccaacgt
     7381 atgtacaagc tgaacgcagg ccgcccaaaa tatcacgcgc ggtattttca accgggcctc
     7441 gcagcggcag cttaacggtt ttaccttctg ctgcgcgata ttccgcaacg ccgccaacgt
     7501 gacgtttcat cgcagactct gagctcatgc cgtagaacaa cataaatttc tcgccgttct
     7561 cctcaacgat gcgaccgccg ctctcttcgt ggcccgccag catgccgcca agcattacga
     7621 aatcggcacc gccgccaaag gctttcgcga catcgcccgg cgtggtgcag ccgccatcgc
     7681 tgacgatcat tccgcccaga ccgtgcgcag catcggcgca ttcgatcacc gcagaaagtt
     7741 gtggataacc gacgcctgtt ttgacgcgag ttgtacaaac agaacctggg ccaatgccaa
     7801 ctttaacgat atcggcaccg gagaggataa gctcctcaca catttcacca gtcactacgt
     7861 tgcctgcaca aatggttttg gtcggccacg cttcacgcgc tttcgcaacg aactgcacga
     7921 agtgttcgga ataaccattc gccacgtcaa tacaaacgaa atttaatgcc gggttcaggt
     7981 cgagaatctg tttagttttt tcgaaatccg catcagacgt gccggtagaa accatcacat
     8041 gtttcagcac atcagcggaa gaattgttga taaacgcttg ccactcttcg acagaatagt
     8101 gtttatgcac agcagtcaga atatcaaaag aagccagcgc agaggccata gaaaatgtgc
     8161 ctacggtgtc catattggcg gcaataatcg gcacgccgga ccagctctga cctgaatgtt
     8221 tgaaggtgaa ttgacgttcc agttcaacat cggaacggct tttaagagtg gagcgtttag
     8281 ggcggatgag aacgtcttta aaacctaact tcagatcttc ttcaatacgc atgtgcggat
     8341 tcctggggtt aatggctata agggtaagag cgcaactcca gtgacgtcat catacgcact
     8401 aatcaatgcg gcgcaagact gcgaaattgt ctttttttac gctacaatcc cataaattta
     8461 cctggtagat agtgagcgaa cgtttcatca atttcgcttt cgtgcgctca tttttaacgt
     8521 tttgataatc atgatgaatt cccgggaata taaattatga ggtatatagt tgccttaacg
     8581 ggaggcattg gcagtggcaa gagtaccgtt gccaatgcgt ttgctgatct cggaattaac
     8641 gtcattgatg ccgatattat tgcgcgtcag gtggttgaac caggtgcacc tgcgctacat
     8701 gccattgctg atcactttgg cgctaacatg attgctgctg atggaacatt gcagcgccgg
     8761 gccttgcgcg agcggatctt cgccaaccag gaagagaaaa actggcttaa cgccctgctg
     8821 catccgctga ttcagcaaga gacgcaacac cagatccagc aagctacttc cccctatgta
     8881 ctatgggttg tgccattgct ggtagaaaac tcactgtata aaaaagcgaa tcgagtgctg
     8941 gtggtggatg tcagcccaga aacgcaactt aagcgcacca tgcagcgcga cgatgtaact
     9001 cgcgagcatg tcgaacaaat ccttgctgct caggcaacgc gcgaagctcg ccttgccgtg
     9061 gcagatgacg tcattgataa taacggcgca ccggatgcta tcgcatcgga tgttgcccgc
     9121 ctgcacgcac actatttgca gcttgcgtcg cagtttgtct cacaggaaaa accgtaatgc
     9181 agacccaggt cctttttgaa catccactaa atgaaaaaat gcgtacatgg ctgcgcattg
     9241 agtttttgat tcagcaactc accgttaatt tacccatcgt tgaccacgct ggcgcgctgc
     9301 atttcttccg taatgtcagc gaattactgg atgttttcga gcgcggcgaa gtccgcactg
     9361 agctgttgaa agaacttgac cggcagcaac gtaaactcca gacctggatt ggcgtgcccg
     9421 gcgtggacca gagccgtatt gaggcattaa ttcagcagtt aaaagcggcg gggagcgtat
     9481 taatctccgc gccgcgtatc gggcaatttc tacgtgaaga tcgtttgatt gctctggtgc
     9541 gtcagcgact gagcatcccg ggcggctgtt gcagctttga tttacctaca ttgcacattt
     9601 ggctgcatct accccaggcg cagcgcgaca gccaggtaga aacctggatt gccagcctga
     9661 acccgcttac ccaagcactt accatggtgc tggatttaat tcgccagtcg gcccccttcc
     9721 gtaaacaaac cagcctgaat ggtttttatc aggataacgg tggcgatgcc gacttgctgc
     9781 gcctgaatct gtcgctcgat tcacagcttt atccgcaaat ttccggtcat aagagccgtt
     9841 ttgccattcg ttttatgccg ctggacagtg aaaacggaca ggtaccagaa cgtctggatt
     9901 tcgaactggc ctgttgctaa ggagtaaaaa tgtcagaaac tattacggtg aattgcccaa
     9961 cctgcgggaa aacggtggtg tggggtgaaa tcagcccgtt tcggccattt tgctccaaac
    10021 gttgtcagct gattgacctc ggagaatggg ctgctgaaga aaaacgaatc cccagcagta
    10081 gcgatctttc cgaaagcgat gactggagcg aagagccaaa gcagtgacat tctatctgat
    10141 gcgacgctgg cctgataaaa cgcgcc
//
LOCUS       DAZFJL010000159        10103 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFJL010000159 DAZFJL010000000
VERSION     DAZFJL010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.161"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(286..2319)
                     /gene="betT"
                     /locus_tag="ACHJ8C_004071"
     CDS             complement(286..2319)
                     /gene="betT"
                     /locus_tag="ACHJ8C_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO5553849.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKNLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2448..3035
                     /gene="betI"
                     /locus_tag="ACHJ8C_004072"
     CDS             2448..3035
                     /gene="betI"
                     /locus_tag="ACHJ8C_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO5553850.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAERRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRHQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            3049..4521
                     /gene="betB"
                     /locus_tag="ACHJ8C_004073"
     CDS             3049..4521
                     /gene="betB"
                     /locus_tag="ACHJ8C_004073"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO5553851.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGCQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTD
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPTKCKAAFEQKVLARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGIEEGARVLCGGDVLKGDSFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQAKSIQVEMAKFQSIF"
     gene            4535..6223
                     /gene="betA"
                     /locus_tag="ACHJ8C_004074"
     CDS             4535..6223
                     /gene="betA"
                     /locus_tag="ACHJ8C_004074"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO5553852.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTTVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVITSKPGVNPLFEA
                     MIEAGMQAGYPRTDDLNGYQQEGFGPMDRTVTPHGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRAAANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPRIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDAEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGKDALPRSTARYFVANGMPVRAKKMSRDLN"
     gene            6419..6919
                     /locus_tag="ACHJ8C_004075"
     CDS             6419..6919
                     /locus_tag="ACHJ8C_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553853.1"
                     /translation="MNEQIKQDIDLIEILFYLKKKIRIILFIIAICMAMVLLFLYNRE
                     IKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKM
                     IYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEY
                     QQNSGQ"
     gene            complement(7162..7857)
                     /locus_tag="ACHJ8C_004076"
     CDS             complement(7162..7857)
                     /locus_tag="ACHJ8C_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO5553854.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLTQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(7850..9277)
                     /locus_tag="ACHJ8C_004077"
     CDS             complement(7850..9277)
                     /locus_tag="ACHJ8C_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO5553855.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRERAAQIRNHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQGAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQENKNG"
     gene            complement(9288..10007)
                     /locus_tag="ACHJ8C_004078"
     CDS             complement(9288..10007)
                     /locus_tag="ACHJ8C_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO5553856.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         2506 a         2749 c         2537 g         2311 t
ORIGIN      
        1 acaaacggtt acattgattt tagttgattt gcgctacgcg ttccccatga ataaagcgtc
       61 atttcaaatt agcaccaata tttacattaa ataagactaa gcttattgaa cattcacaac
      121 agtccaaacg tagtttttgg gcttcaatag ttcttttctt ttttattatc tgttgcctgg
      181 caatcatcaa tcgatactaa gggcttaacc gtacagatat aacgtatcaa gaggtaagag
      241 aaaccgcgcc atatgcagac atggcgcggt tttatgcaat aacaatcacg cgtccgggaa
      301 catcacgcta tggcccggcg cttcacgatg gagatggata aagttaaggt gccgctcgta
      361 ctggtcgaga atatcggtga tcacctgctc tttgctgtag tccatcaggt cgttgccctg
      421 gctgccttct aacaggaagg tttccagccg gtagtaggtc gatttcccgc tacgtgcgcg
      481 gtaggtaaag cccggcaccg aatattgctg cggccaaatc tgatagacaa agttctgctc
      541 ttcgcccata tgcaccaaca aatccagatg ccccaactgt tgtccctctt ccggcggcag
      601 gttttttagc tccacgtacg cgccgcgcaa ccgcagttcc tgcgccactt cttccattgc
      661 cggataacag accgtctcca tcatctgttt agtgtaacgc gtgcccggat aattcatcag
      721 gcgcgagaga cgttttttcc agctcaggcg atcctgaagc cccagcggtc gcggtgcggt
      781 atcgcggttg gcactttcac ggcggtaatc ttctaccttc agagatttat acaaccccgc
      841 catcacgaag aagatcacaa agctgaacgg caggcccata atcaccgtgg tgttttgcag
      901 cgcggatatc ccgttggtca tcagcatgcc tagcgtcagc aggccaatcg ccaccgacca
      961 gaagacgcgc agccagccgg gtgcgtcgct gttgatatct ttaagctgcg aggtgaaatt
     1021 ccccagcacc aacgcaccgg agtccgccga ggtcacataa aacagcaggc cagtaatggt
     1081 ggcgacggag gcgctaaagg taaacgccgg atactgcgcc agcaggctgt agaagccgcg
     1141 ctccggatgg accatcgctt cctcggcaaa tgccgcgccg ccgtggatga tttcatacag
     1201 cgcgctattg ccgaataccg agagccataa cagcgtgaag gtaaacggaa taatcaacgt
     1261 gcccagcacg aactggcgaa tggtacgccc acgcgagata cgcgccagga acaagccgac
     1321 aaacggcgac catgccaccc accatgccca gaagaagagc gtccagttat tcatccactc
     1381 aaccggacgg tcgaaggcaa aactgttgag cgtcatgccc ataaagcgat tcacatagtc
     1441 gccaacattc agcaccagtg cattaagcag gaacgaggtg tcgcccataa acaacacgaa
     1501 caggatcaat cccagcgcca gcgcaacgtt gagttccgat aacacgcgaa tgcccttatc
     1561 aacaccggat gtcaccgaga tagtggcgat gatcaccgac aaggcgatca gtgccgcttt
     1621 cgccgccatc gaatcgggaa tatcaaacag tacgctcaag ccatagttaa gctgcaccac
     1681 accgataccg agcgtagtgg caataccgaa gatggtgccg atcaccgcag caatatccac
     1741 tgagtgacct atcggcccgt taatccgttt accgaagatc gggtacagcg ccgagcggat
     1801 agtgagcggc aaattataac gatagctaaa gtatccgagc gccatgccca tcagcgcata
     1861 catcgaccag ccggttaagc cgtagtgaaa cagcgtccag accatcgcct gacgcgcggc
     1921 ctcaatcgtc tgtcccgcgc cttccggcgg ctgcatatac tgcgttaccg gttcggctac
     1981 ggagaagaac atcaggtcga taccgatccc ggcagcaaac agcatcgccg cccaactcag
     2041 cagactgaat tccggttttg attgttctgg cccgagcttc accgaaccaa aacgcgaaca
     2101 agcgatacag accacaaaga caatatagag cgttgccgcc agcagatagt accaaccgaa
     2161 ggttttagaa acccagtcca gcgtgcggcc aatccacagg gccgagaagt cgcgaaacag
     2221 gatcgttgtc agggaaaaca acaaaatcag tccggcggaa gtgtaaaaca ccaccggatt
     2281 gattttgtcc ttttccctgc tgtgtgaaag gtctgtcatc cagtatcccc actgttattg
     2341 ttaccattta aaatcaaatt agtaacaatt aagacacatt ttatattgaa cgtccaatca
     2401 ataaccgctt taatagataa acaacgctga tgaatggagt ggcgaaaatg cccaaattgg
     2461 gaatgcagtc gatccggcgc agacaactga tcgacgccac gctggaagca ataaatgaag
     2521 tgggcatgca cgatgcaacg atcgcgcaga tcgcccgccg tgcaggcgtt tctacgggga
     2581 tcatcagcca ctatttcagg gacaaaaatg gtctgctgga agcaaccatg cgcgatatca
     2641 ccagtcagct gcgtgacgcg gttttgaatc gattacatgc acttccgcag ggcagtgcag
     2701 agcggcgatt acaggcgatt gttggcggaa acttcgatga aacccaggtg agcagtgcgg
     2761 cgatgaaagc ctggctggcc ttctgggcca gcagtatgca tcagccgatg ctctatcgct
     2821 tgcagcaggt cagcagccgt cgcttgctgt cgaacctggt gagcgagttt cgccgcgaat
     2881 tgccgcgcca ccaggcacag gaagcgggct acggccttgc cgcgctgatt gacggattat
     2941 ggctgcgcgc ggcactgagc ggcaaaccgc tggataaacc tctcgcacac tcgctgaccc
     3001 gtcactttat tactcagcat ttacccaccg attaaccgag gagacgtgat gtcccgaatg
     3061 gcagaacagc agctttatat acatggtggt tatacctccg ccaccagcgg tcgcaccttc
     3121 gagaccatta acccggccaa cggtaacgtg ctggcgaccg tgcaggccgc cgggcgtgag
     3181 gatgtcgatc gcgccgtgaa gagtgcccaa caagggcaaa aaatctgggc ggcgatgacc
     3241 gctatggagc gctcgcgtat tctgcgtcgg gccgttgata ttctgcgtga acgcaatgac
     3301 gaactcgcaa aactggaaac ccttgacacc ggaaaagcat attcggaaac ctcaaccgtc
     3361 gatatcgtta ccggtgcgga cgtgctggag tactatgccg ggctgatccc ggcgctggaa
     3421 ggctgccaga tcccgttgcg tgagacgtca tttgtttata cccgccgcga accgctgggt
     3481 gtggtggcgg ggattggcgc atggaactac ccgatccaga ttgctttgtg gaaatccgcc
     3541 cccgcgctgg cggcgggtaa cgcgatgatc ttcaaaccga gcgaagtcac cccgcttacc
     3601 gcgttaaagc tggctgaaat ttacagcgaa gcgggcctgc cggacggtgt atttaacgtg
     3661 ttgccgggcg tgggcgcgga gaccgggcaa tatctgaccg atcatccggg cattgccaaa
     3721 gtgtcattta ccggcggcgt cgccagcggc aaaaaagtga tggctaactc ggcagcctct
     3781 tccctgaaag aagtgaccat ggagctgggc ggtaaatcac cgctgatcgt tttcgacgat
     3841 gcggatctcg atctcgccgc cgatatcgcc atgatggcga atttcttcag ctccggtcag
     3901 gtgtgtacca acggcactcg cgtcttcgtt ccgacgaaat gcaaagccgc atttgaacaa
     3961 aaggttctgg cgcgcgttga gcgcattcgc gcgggcgacg ttttcgatcc gcaaactaac
     4021 ttcggcccgc tggtcagctt cccgcatcgc gataacgtgc tgcgctatat cgccaaaggc
     4081 atagaggaag gcgcgcgcgt actgtgcggc ggcgatgtac tgaaggggga tagcttcgat
     4141 aacggcgcat gggttgcacc gaccgtgttc accgattgca gcgacgatat gaccattgtg
     4201 cgcgaagaga ttttcgggcc ggtgatgtcc attctgacct acgagtcgga agacgaagtc
     4261 attcgccgcg ctaacgatac cgactacggt ctggcggcgg gtatcgtgac ggcagacctg
     4321 aaccgcgcgc atcgcgtcat tcatcagctg gaagcgggta tttgctggat caacacctgg
     4381 ggcgaatccc cggcagagat gcccgttggc ggctacaaac actccggcat tggtcgcgag
     4441 aacggcgtga tgacgctcca gagttacacc caggcgaagt ccatccaggt tgagatggct
     4501 aaattccagt ccatatttta accaggaggt ttatttgcaa tttgactaca tcattattgg
     4561 tgccggctca gccggcaacg ttctcgctac ccgtctgact gaagatccga acacaaccgt
     4621 gctgctgctt gaagcaggcg gcccggacta tcgctttgac ttccgcaccc agatgcccgc
     4681 cgcgctggcg ttcccgctac agggcaaacg ctataactgg gcctatgaga cggaacccga
     4741 accgtttatg aacaaccgcc gcatggagtg cggacgcggc aaaggtctgg gcggttcatc
     4801 gctgatcaac ggtatgtgct acatccgtgg caatgcgctg gatctcgata actgggcaca
     4861 agaacctggt ctggagaact ggagttatct cgactgcctg ccctactacc gcaaggccga
     4921 gactcgcgat gtgggcgaga acgattacca cggtggcgat ggcccggtga gcgtcatcac
     4981 ctccaaaccc ggcgtcaatc cgctgtttga agcgatgatt gaagcgggca tgcaggcagg
     5041 ctacccgcgc acggacgatc tcaacggcta tcagcaggaa ggttttggcc cgatggatcg
     5101 caccgtcacg ccgcatggcc gtcgcgccag caccgcgcgt ggctatctcg atcaggccaa
     5161 atcgcgccct aacctgacca ttcgtactca cgccatgacc gatcacatca tttttgacgg
     5221 caaacgcgcg gtgggcgtcg agtggctgga aggcgacagc accattccga cccgcgcggc
     5281 ggcgaataaa gaagtgctgt tatgtgcagg cgcgattgct tcaccgcaga tcctgcaacg
     5341 ctccggcgtc ggcaacgctg aactgctggc ggagtttgat attccgctgg tgcatgaatt
     5401 acccggcgtc ggcgaaaatc ttcaggatca cctggagatg tatctgcaat acgagtgcaa
     5461 agaaccggtt tccctctacc ctgccctgca gtggtggaat cagccgagaa tcggtgcgga
     5521 gtggctgttt ggcggcaccg gcgttggtgc cagcaaccac tttgaagcag gcggatttat
     5581 tcgcagccgt gaggaatttg cgtggccgaa tattcagtac catttcctgc cagtggcgat
     5641 taactataac ggctcgaacg cagtgaaaga gcacggcttc cagtgccacg tcggatcgat
     5701 gcgctcgcca agccgtgggc atgtgcggat taaatcccgc gacccgcacc agcatccggc
     5761 gattctgttt aactacatgt cgcacgagca ggactggcag gagttccgcg acgcaattcg
     5821 catcacccgg gagatcatgc atcaaccggc gctggatcag tatcgtggcc gcgaaatcag
     5881 ccccggtgtc gaatgccaga cggatgagca gctcgatgag ttcgtgcgta accacgccga
     5941 aaccgccttc cacccgtgcg gtacctgcaa aatgggttat gacgagatgg cagtggttga
     6001 cgccgaaggc cgcgtacacg ggctggaagg cctgcgcgtg gtggatgcgt caattatgcc
     6061 gcaaattatc accggcaatt tgaacgccac gacgattatg attggcgaga aaatagcgga
     6121 tatgattcgc gggaaggatg cgttgccgag gagcacggca agatattttg tggcaaatgg
     6181 gatgccggtg agagcgaaaa aaatgagtcg tgatttgaac taactcagga acctatcaga
     6241 ttgagagtta ccgttctact atgaggaccg aatccttata taaaacactg aggtaactct
     6301 catgcttcat atccagtatt catgatgctg gtttttcgtt gatatttaac ccgaacattc
     6361 ctgatacaga ctattaatac aatattatct gcgacagatt tcattttctg gatatattat
     6421 gaatgaacaa ataaaacagg atatcgatct gattgagatt ttattttatc tgaagaaaaa
     6481 gattcgtatt atccttttta ttatagctat ctgtatggca atggtgctgt tgtttctgta
     6541 taacagagaa ataaagctgg aatggtcagg agataagaga gatttaccaa ctgctgaagc
     6601 ggaaatatct cgcgtgcagg cgtctatcat caaatggtat gcgtcagaat accataatgg
     6661 caggcaagtt ctcgatgaga tacaaacgcc ttcagcaatt aacagtgagc tttatacaaa
     6721 aatgatatac ctgaccagga actggtcact gtatccgaac ggtgatggct gtgtcactat
     6781 aagctcacca gaaataaaaa ataaataccc tgctgccatt tgcctggctc tgggattttt
     6841 tctaagtatt gtgatttctg taatgttttg ccttgtcaaa aaaatggtag atgaatacca
     6901 acaaaactct gggcagtaat aaacagcatt ctgacagaga gtgcctgtat aaaactatag
     6961 tgacaatgac cgggaaggga gcccgtcttc aggaatggat tcctgtcttt ttcactgtaa
     7021 atatcctggc attattaatg tcaggatatt tacagaagag cagcataacc atgacggaac
     7081 ggggttaagt caggttgtcc ttaacgctat tcacaacctc gtataccgcc ttcaggttta
     7141 tgtccagact acatatctct ctcagcaatc ctcaataatc agatacaccg ctttcaccgg
     7201 accatgaacc ccgacgactt tgataagctc aatgtccgcc gttgaactgg gaccactgat
     7261 gatgttaata catgacggca ttcgttcacc ggcctgcgct ttctgatgca atttttctgc
     7321 aagttgcgct acacgcggta ggattgtgct tttacgcagg ataaaaagag aagattccgg
     7381 gagcaggctc aatgaacgcc cgcgcccggc ggcggaaaaa agaaccacgc ctcccgattc
     7441 ggttaaacca tattcagcat atacgacgcc cactttagcc tgctctgctt gtgatatgtt
     7501 ctcaatacct ttcgccggat cccaaacaac ggcattgcat tcctgctgca aacgttcgct
     7561 aatccccaat tcctccagcc tcgtgtcacc actaatcacg accgactgat ctcccagctc
     7621 tttacacaga cgtattgcag cttctgccgc cttcgcctcg ctggtcagtt cacagcgcgt
     7681 caacataaca tcgctggcaa actggataaa cgcgtcacag cgctgctgtt gggtaagttg
     7741 ggtaagccgc tcgttagcat agttgttaag cggcgcatct tctgcttgcg gttcaagtcg
     7801 cagcgggcga cccagtgcct gagcaacgtt attcaaaaat tcgctccgat tatccattct
     7861 tattctcctg cgcctgatgt ttcttaaacc aactacggaa actctctccg tcagcttcag
     7921 gaagatcgcg tgcttccatc cagtcgctaa tcgcgccaaa tttgagtggt gttttgccgc
     7981 cattgataaa ccagcttgcc gcatgagcac cagccatcat cccaactttc cacaatcctg
     8041 gatgactatt ggcataagcg aacattttta tcgcccgttg ctctgctttt gcggtgatcc
     8101 ctttttcagc catcacccga cgatgacgca aaatcagttt tgacagcgga atacgcaccg
     8161 gacacacgct gtcacaagcg gtgcataaag agcaggcgta gggtaaatct ttaaaatctt
     8221 tatagccgcc aagtaacggg gaaatcaccg caccaattgg ccctggataa atagagccat
     8281 atccatgacc gccaatatgg cgatatgccg gacaagtatt catacaagcc ccgcaacgaa
     8341 tacagcgcag cacatcccga aattcagagg ccagcacctc agaacgcccg ttatcgacaa
     8401 taaccagatg aaactcttca ggaccatcaa cgtgccccgc ttcgcgcggt cctgtcagcc
     8461 aggtgttgta tcccgtcaaa cgtgcaccaa cggcactgcg cgccagcatg gtgatcaata
     8521 catctacctc tgcaaacgtg ggagcaatac gctccattcc catcactgca atatgcgttt
     8581 taggcagcgt ggtacacatt cgcgcattac cttcattggt caccaggcat accgaaccgg
     8641 tctctgccac cgcgaaatta cagccggtaa tacctatttc agcactgagg aaatcttcgc
     8701 ggattttttg ccgaatgaat aaggtcatcg cttcaggcgt ttccggcccc tcatagccca
     8761 gacgttcgtg tagcactcga cggatctgat ggcgatcttt atgaattgcc gggaccacaa
     8821 catgagaggg cggatcttga tccagctgga gaatgtattc acccagatcg gtttcaatca
     8881 cctgaatccc ggcaccctgc aacacatgat tgacaccaat ctcttcggtc accatcgatt
     8941 tagatttcac caccttccgg gcatttttgc gttgggcaac ctgtaaaatg tagcgggtgg
     9001 cgtcttcttt ggtttttgca aaatagacgt gaccgccgtt ttgtgtcact ttttctgaga
     9061 gctggtacag ataagcgtcg agattactca gaacatgatt acgtatctgg gcggcccgct
     9121 cgcgccactc ctcccagtgc cccaattcat cgaccatttt ttgccgattt gccccaatac
     9181 gctgctgcgc gtttgccacc gctttgcgca tgatcggatc ttcaatttgc tgacgaatgc
     9241 gtgtcttaaa atctgtatta ctggttttga tcgacatctt tatatcctca gcggctcatc
     9301 agcacttcag caatatgcat cactttgact ttctgccctt cccgttgtaa tcgtccactg
     9361 atgttcagca ggcaactcac gtcagcacca attaaatact cagggcggac ttccatcagg
     9421 tgcgcaacct tttctttcac catctcgccg gatatttcgg ccattttgac cgagaacgtg
     9481 ccgccaaatc cgcagcaggt atcctgttca gcaaaggtca acagctccag tccacgcaca
     9541 tttttcagca gcgtaagtgg ttcgtccttc acccccagct tacgggccag gctacaagat
     9601 gggtgataca ccgctctccc ttgcaaactg gcacctacat cgactacccc taatgtatta
     9661 acaataaaag aggtgagatc ctgcatacgc gcggcaacct tttcggcacg tgatgcccat
     9721 tcaggttcat ccgccagata cgtcgggtaa ctttttacgg cataggtgca agagccagcc
     9781 ggtgaaataa tgggatcgtc gttatcctcc agtgcggcga tcagattttt catccctgga
     9841 atcgcttctt tgatataacc actattgatc gcaggctgac cgcagcatcc ctgtttctcc
     9901 gggaaattta cgcgacagcc gagtttttcc agtagcagca cggagtctcg tgccattctt
     9961 gatttcaggg cgtcaccaat acaggtgaca aagaaattga cattcacaac taatactcca
    10021 ttacttcatg cccatttatg cccgatggtt ccccccttcc gggggatcaa tcatcaataa
    10081 atatggcgtt cttttttaat tgg
//
LOCUS       DAZFJL010000160        10048 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFJL010000160 DAZFJL010000000
VERSION     DAZFJL010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.162"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            307..1077
                     /gene="yjjP"
                     /locus_tag="ACHJ8C_004079"
     CDS             307..1077
                     /gene="yjjP"
                     /locus_tag="ACHJ8C_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO5553857.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFLASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1068..1541
                     /gene="yjjB"
                     /locus_tag="ACHJ8C_004080"
     CDS             1068..1541
                     /gene="yjjB"
                     /locus_tag="ACHJ8C_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO5553858.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1649..2188
                     /gene="dnaT"
                     /locus_tag="ACHJ8C_004081"
     CDS             1649..2188
                     /gene="dnaT"
                     /locus_tag="ACHJ8C_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO5553859.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFDNNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQTAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTAEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2191..2928
                     /gene="dnaC"
                     /locus_tag="ACHJ8C_004082"
     CDS             2191..2928
                     /gene="dnaC"
                     /locus_tag="ACHJ8C_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO5553860.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2977..3471
                     /gene="yjjA"
                     /locus_tag="ACHJ8C_004083"
     CDS             2977..3471
                     /gene="yjjA"
                     /locus_tag="ACHJ8C_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO5553861.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNN
                     TTQEGGWSLASLTNLLSNGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3725..6016
                     /gene="opgB"
                     /locus_tag="ACHJ8C_004084"
     CDS             3725..6016
                     /gene="opgB"
                     /locus_tag="ACHJ8C_004084"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO5553862.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLAAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKAIWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6156..7178)
                     /gene="lgoD"
                     /locus_tag="ACHJ8C_004085"
     CDS             complement(6156..7178)
                     /gene="lgoD"
                     /locus_tag="ACHJ8C_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFO5553863.1"
                     /translation="MSTMKVLICQEIKNLVWEVRERPVPGDNEALIKIKTVGICGTDI
                     HAWAGNQPFFSYPRVLGHEICGEIIESGKNIHNFNIGQQVAVIPYISCQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APSEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7317..8222
                     /locus_tag="ACHJ8C_004086"
     CDS             7317..8222
                     /locus_tag="ACHJ8C_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO5553864.1"
                     /translation="MSRSQNLRHSVINQVIEDMARGNIPSPLPSQSGLAEMYNISRTT
                     VRHILQHLSACGVLTLVGKNYVIARKPEQNDGFDCITASLKEQNRLFEQAFFTMINLR
                     QLRAGESFSELQLARAASVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDDDPRWLQAKTMLERHRTLRDSIGSNFRMFSQLDRDFHA
                     MLLSAADNIFFNQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     AITALRNHLDTAKQSMIRSIRQHHR"
     gene            complement(8393..10045)
                     /locus_tag="ACHJ8C_004087"
     CDS             complement(8393..10045)
                     /locus_tag="ACHJ8C_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO5553865.1"
                     /translation="MEILLTILILTLVVSLTSVLTRLSPVQIPLPLIQIAAGAVLAQP
                     IFGLHVEFNPELFLLLFIPPLLFAESSKIQPKELIKHSREIISLALVLVLITIFGVGY
                     VIHLLLPNVPLIAAFALAAVLSPTDAVALLGIVGKGRISKNIQEVLEGEALMNDASGL
                     VALKFAVAVTMGTMEFSVHGATIAFFVVALGGIAVGIAVTWLYGKGLLLISRYAHDEP
                     SIQIVLMLLLPFIVYLVAEHFGLSGILAAVAAGLTTSRIGVVHHAPIRLRIKAKSSWS
                     MLEYVFNGMVFLILGLQLPGIISESLIRAENDPLVDTGSLLLDVIWIYLALMVVRLGW
                     LWLMRLYSRKRPGKHPMLFAGYKTRHMLLATFAGVRGAITLAGGLSIPLLLPDGTPFP
                     GRYLLVFLATGVILFSLVCGIIILPLLLGKNVGIFSDNRQKEEEAADLFMAKMAILSV
                     QKTEQRLLRARTENLDNELIMETGARVIGYMRHSEEDMSPNDNSLHMQRAKLERTFNL
                     AALNAKRAAIYQMRARQEVSDETLAKIVSELDVLETLIMDSR"
BASE COUNT         2637 a         2585 c         2377 g         2449 t
ORIGIN      
        1 acacaatcaa tatttattca ataaaatctg atggttttaa cttgttaaca taaaaaaacg
       61 cgcccagcta tctattatcc tgactcgtaa aggccaaata acaaattcat tatagtgata
      121 atattcatgc gatagtttgt ttacggcgag agataattcg ctttttatca ccgagcatct
      181 cccgataacc aggattaatc agcgcaattt tgatcttaac ctaccgcaaa acaaccgtgc
      241 ttcatgatac aaatggattc tcattatctg aacaatacgc agcacgtcta tgacaaaggg
      301 cgagttatgc aaactgagca acagcgagcc gtaacacggc tttgtatcca gtgtggatta
      361 tttcttttgc aacatggtgc ggaaagcgcg ttggttgatg agctttcctc acgactgggt
      421 cgggcgttgg gaatggacag cgtcgagagt tctatctctt cgaacgccat agtgctgaca
      481 accattaaag atgggcaatg cctgacatcg acacgtaaaa atcacgatcg cggcattaat
      541 atgcatgtgg tgactgaagt ccagcacatt gtaattctgg cggagcatca tctgctggat
      601 tacaaaggcg tagagaaacg atttagccaa attcagccat tacgttaccc aagatggctg
      661 gtagccttaa tggttggcct ttcctgcgcc tgtttctgta aactcaataa aggtggctgg
      721 gatggtgccg tcatcacctt cctggccagt acggccgcga tgtatatccg ccagctgctg
      781 gcacaacgtc atcttcatcc acagatcaac ttttgcctca ccgctttcgc cgccaccacc
      841 atttccggat tgcttttgca acttcccact ttcagcaata cccccaccat tgcgatggcc
      901 gccagcgttc tgctgctggt gccgggcttt ccgttgataa atgccgtcgc cgatatgttt
      961 aaaggccaca tcaataccgg actggcacgc tgggcaatcg ccagtctgct gacactggct
     1021 acctgtgtcg gcgtagtaat ggcactgacg atttgggggc tacgcggatg ggtgtgatcg
     1081 aatttctgtt agcgttggcg caggatatga tcctcgccgc cattcctgcg gtcggctttg
     1141 cgatggtgtt caacgttccc gttcgagcgt tacgctggtg tgcgctgctt ggtgcgatag
     1201 gtcatggttc ccgaatgatc ttaatgacca gcgggctgaa tattgaatgg tcaaccttta
     1261 tggcctctat gctggtcggc accattggta ttcaatggtc gcgctggtat ctggcgcatc
     1321 cgaaagtgtt taccgtggcg gctgttatcc ctatgttccc tggcatatca gcttataccg
     1381 caatgatttc ggcggtaaaa atcagccagc taggttacag cgaaccgttg atgattaccc
     1441 tgttaaccaa ctttcttaca gcttcatcga ttgttggtgc gttatccatc ggtctttcca
     1501 ttcctggatt atggttgtac cgcaagcgcc ctcgcgtata aaattgcctc acttaaagca
     1561 cgcggatgag agacagcctc ctctcctccg tgtgttacta taaaagttat ctcccttctc
     1621 gttcatcgtt ccatatttga gaaacagtat gtcttccaga gttttgaccc cggacgtcgt
     1681 tggtattgac gccctggtac acgatcacca aaccgttctg gcaaaagctg aaggcggtgt
     1741 ggttgccgta tttgacaaca acgccccggc gttttatgcc gtcacgcctg cacgcctggc
     1801 tgaactgctg gcgctggaag aaaagctggc gcgtccggga agtgacgtgg ccctggacga
     1861 tcaactctat caggaaccgc aaaccgctcc cgttgccgta cccatgggga aatttgccat
     1921 gtatccggac tggcaacccg atgccgactt tatccgcctg gcggcgctat ggggcgtggc
     1981 actaagagag ccggtgaccg ccgaagaact ggcgtcattc attgcctact ggcaggcgga
     2041 aggcaaagtc tttcaccatg tgcagtggca acaaaaactg gcgcgcagcc tgcaaatcgg
     2101 tcgtgccagc aacggcggac tgccgaaacg agatgtgaat acggtcagtg aacctgacag
     2161 ccaaattcca ccaggattca gagggtaacg atgaaaaacg ttggcgactt gatgcaacgc
     2221 ctgcaaaaaa tgatgcctgc ccatatcaaa cctgctttca aaacgggtga agaactactg
     2281 gcctggcaga aagaacaagg ggcgatccgc tccgccgctc tcgaacgtga aaatcgggcg
     2341 atgaaaatgc agcgcacctt taaccgctcc ggtattcgtc cactgcatca gaactgctcc
     2401 tttgagaact atcgcgttga gtgtgaaggg caaatgaatg cgttaagcaa agcgcgccag
     2461 tatgtcgaag agtttgacgg caacatcgcc agcttcatct tttctggtaa gccaggaacc
     2521 ggcaaaaacc atctggcggc ggcaatctgc aacgagctgc tgctacgcgg taaatccgta
     2581 ttgatcatca ccgtggccga tattatgtcg gcgatgaaag ataccttcag gaatagcggt
     2641 accagcgaag aacaactgct taacgatctg agtaacgttg atctgctggt gatcgatgag
     2701 atcggcgtgc agaccgaatc gaaatacgaa aaagtgatca tcaaccagat cgtcgatcgc
     2761 cgctcttctt ccaaacgccc aaccgggatg ctgaccaaca gtaatatgga agagatgacc
     2821 aagttgctgg gcgaacgcgt gatggaccgt atgcgcctgg gtaacagttt gtgggtgatc
     2881 ttcaactggg atagctaccg tagccgtgta acaggtaaag agtattaatc cccgtttctg
     2941 gcggtatact gcgacacaat tcaaccttga gtaatgatga tgaaaactat taaacatctt
     3001 ctgtgctgtg ccattgccgc cagcgcatta atttccaccg gggtgcatgc tgcgtcctgg
     3061 aaagatgcgc tctccagcgc cgccagcgaa cttggcaacc aaaacaacac gacacaggaa
     3121 ggcggttggt cgctcgcgtc attaactaac ttgcttagca acgggaacca ggccttaagc
     3181 gcagataaca tgaacaacgc cgcaggcatt ctgcaatact gcgcgaagca aaagctggct
     3241 tcggtaaccg atgccgaaaa catcaagaat caggtactgg aaaaactggg cctgaacagt
     3301 gaagagcaaa aagaagacac caactatctg gatggcattc agggtttgct gaaaaccaaa
     3361 gatggtcagc aactcaatct ggataatatt ggaacgactc cgctggcaga aaaggtgaaa
     3421 accaaagcct gcgatctggt gttaaagcag gggctgaact tcatttcctg aaccgcgaaa
     3481 cgccgcgttt tagctgttac ccggctgacg cggctctctc ctgccctttt ccctgctcct
     3541 ggacggtttt acccctgtta cacgggaagc gaccgcgatc aaaaaagtct gtttataaac
     3601 taattcctaa caacatcgca tttttttgac actagaattg cagcaatatg gcgacgccat
     3661 tacattgtgc ttctcaaaaa gcttaaacgt tggctaaacg gcccggttta gtgaggatcg
     3721 cccgttgtca gaactactct ctttcgccct ttttctcgcc tctgtgctga tttacgcatg
     3781 gaaagcggga cgtaacacct ggtggtttgc agccacatta acggtactgg ggctatttgt
     3841 cgttttaaat atcaccctgt ttgccagcga ctattttact ggcgatggta ttaacgacgc
     3901 ggttctctat accttaacca acagcctgac cggtgctggt gtcagcaaat acatcctgcc
     3961 tggtatcggc attgtgctgg ggctggcggc ggtgttcggt gcgctgggct ggatcctgcg
     4021 ccgtcgtcgc catcatccgc accattttgg ttacagcctg ctggcactct tactggcgct
     4081 gggttcagtg gatgccagcc cggcatttcg tcagataacg gaactggtga aatcccagtc
     4141 acgcgacggc gacccggact ttgcggctta ttataaagag ccgtcgaaaa ctatccctga
     4201 cccgaaactc aacctggttt atatctacgg cgaaagtctc gagcggacct attttgataa
     4261 cgaggctttc ccggatctca cgcctgaact gggcgcgttg aaaaatgaag gcctggattt
     4321 cagccacacg cagcagctgc caggaacgga ttacacgatt gcgggcatgg tggcttctca
     4381 gtgcggcata ccgctgtttg ccccatttga aggcaacgcc tccgcctctg tctccagctt
     4441 cttcccgcag aacatctgtc tgggcgatat cctgaaaaac tcgggttatc agaactattt
     4501 cgtgcagggc gcgaatctgc gttttgccgg taaagatgtg ttcctgaagt cgcatggctt
     4561 cgaccactta tacggctcag aagagctgaa aagtgtggtg gccgacccgc actatcgcaa
     4621 cgactgggga ttctacgacg ataccgttct cgatgaagcg tggaaaaagt ttgaagagct
     4681 ttcccgctca ggtcagcgat tctcactgtt taccctgaca gtcgataccc atcacccgga
     4741 tggttttatc tcgcgtacct gtaaccgcaa aaaatatgat tttgacggta agccgaatca
     4801 gtcattcagc gcggtaagtt gcagccagga gaatattgcg acgtttatca acaaaatcaa
     4861 agcgtcaccg tggtttaaag ataccgtcat cgtcgtctct tctgaccatt tagcgatgaa
     4921 caatacggcg tggaaatacc ttaataagca ggatcgcaat aacctgtttt ttgtcatccg
     4981 tggcgacaag ccgcagcaag agacgctggc agtgaagcgt aatacgatgg ataacggcgc
     5041 gacggtgctg gacattctcg gtggcgataa ctatctcgga cttggccgta gcagtttatc
     5101 cgggcagtcg atgtcggaaa tcttcctcaa tatcaaagag aaaacattgg cgtggaagcc
     5161 ggatatcatc cgcctgtgga aattccctaa agagatgaaa gagttcacca tcgaccagca
     5221 gaaaaacatg attgccttct caggtagcca tttccgtttg ccgctgctgt tgcgggtttc
     5281 agacaaacgc gtggaaccgc tgccggaaag cgaatactca gcaccgctgc gtttccagct
     5341 ggctgatttc gctccacgcg acaatttcgt ctgggttgac cgttgctata agatggcaca
     5401 actctgggct ccggaactgg cactttccac cgactggtgt gtctcgcaag ggcagcttgg
     5461 cggtcagcaa attgttcagc atgttgacaa agcaatatgg aagggcaaaa cggcatttaa
     5521 agatacggtc atcgacatgg cgcgttacaa aggcaatgtc gatacgctaa agattgttga
     5581 taacgatatt cgctacaaag ccgacagttt catctttaac gtcgctggtg cgccagaaga
     5641 ggtgaaacag tttagcggga tttctcgtcc agagtcgtgg ggccgctggt ccaacgcaca
     5701 gctgggcgat gaagtaaaaa tcgagtacaa gcatccgctg ccgaagaaat ttgacctggt
     5761 gattaccgcc aaagcatacg gcaataacgc cagccgtcct attccggtac gcgtaggcaa
     5821 tgaagaacaa acccttgtgc tgggcaatga agtgaccacc accacactgc atttcgataa
     5881 cccaaccgat gccgacacac tggtaattgt gccaccggaa cctgtctcaa ccaacgaagg
     5941 gaatatcctc gggcactcgc cgcgtaagct cgggatcggc atggtggaaa ttaaagtggt
     6001 agaacgtgaa gggtaattcc tgactgatgt gtaggtcgca taaggtgcga cctacacacc
     6061 ttctccggcc tacaaaacat tattgagaaa cagaaaaaaa acagcgccca acacttcccc
     6121 ggacgctgca tggcaacttt aataatctcg ccttatcaga aagtaattac gcccttaatc
     6181 aactcacgat tgttaatcac atcgcgctcg taggtttctg ccagcgtggc gaacggatag
     6241 cggtgggtta acatcatgtc ggcagtgatt ttcccttccg ccatcaatcg accgacttta
     6301 gcgaaatctt ccggcgtggc gttgcggctg cccatcatcg tcgtttcttt tttatggaat
     6361 tccggatcgg agaactgcaa ctcaccttta aacaggccga caaataccac cgtgccgccg
     6421 tgacgaatca gatttacggt gttattcatc gcatgttgat tacctgtcgc gtcaatcact
     6481 ttctgcgcca gcgaaccacc aaactgcgcc cgcagctgcg cgtcaaaatc ttcggctgac
     6541 gggtccagaa caggtaattc cagacgcgtt gccacatgtt cacggcgcgc cggactggta
     6601 tccgccacca ccacctgcgc gccatcggct ttagcgattg ccgccgcgcc cagaccgatt
     6661 ggccccgccc cgaccaccag cacctgctcg ctgggtgcaa tggctgcacg acgcaccgca
     6721 tgagcgctaa tagcgaaagg ttcaatcaat gctgccgcct gcgggtcaat accgtctgcg
     6781 ggcaaaatgt tcgccaccgg cacgctcaaa tactcactaa aaccgccatc ctgatgcacg
     6841 ccaatgactg aaattttttc acagcaattg gtacgaccgc ttttacacgc cgggcattgc
     6901 tgacaagaaa tatacggaat taccgcaact tgctgtccga tattaaaatt gtgaatgttt
     6961 tttcctgatt caataatctc cccacatatt tcatggccta aaacgcgtgg ataactgaaa
     7021 aatggctgat tgcccgccca ggcatgaata tctgtaccgc aaatcccgac agttttaatt
     7081 ttaattaatg cttcattatc gcctggcacc ggtctttccc ggacttccca tactaaattc
     7141 tttatttcct gacaaattaa tactttcatt gtggacatga ttcaccgctc cttttatttt
     7201 atgactttca gatatcgtcg aatttataag agcgcctgaa tttacccata aaatatgcgg
     7261 atgatcgcgc ataaaaatgt tttaaatcgg cttttaatgc cggactggag ggaagaatga
     7321 gtcgttcgca gaatttacgt catagcgtca taaatcaggt gattgaagat atggcgaggg
     7381 gcaatattcc ttctccgctg ccttcacaaa gcggactggc agaaatgtat aacatcagtc
     7441 gcactacggt acgccacata ctccagcatt taagcgcatg tggcgtgctc accctggtgg
     7501 ggaaaaatta tgttatcgcc cgcaaacccg aacagaacga tggatttgat tgcatcacgg
     7561 cctcgctgaa agagcaaaac cgtcttttcg agcaggcatt tttcaccatg attaacctgc
     7621 gccagttacg cgccggagaa agcttttccg aacttcagct ggcgcgagcg gcaagcgtca
     7681 gtccggtagt ggtacgagaa tatcttttga aattcggtcg ttataatctt attcaaagcg
     7741 aaaagcgcgg ccaatggagc atgaagcagt tcgatcaatc ctacgccgag cagctgtttg
     7801 agctacgcga aatgctcgaa acacattccc tgcaacactt tctcaacctt cccgatgatg
     7861 accctcgctg gctgcaagca aaaaccatgc tcgaacgcca ccggacgctg cgcgacagca
     7921 ttggcagcaa ctttcgaatg ttttctcaat tagaccgtga ttttcatgcc atgctgttat
     7981 ccgctgctga caacatcttt tttaatcaat cacttgaaat tatatcggtg atctttcatt
     8041 ttcactatca atgggatgaa agcgatctca aacagcgcaa tatcattgct gttgatgaac
     8101 atatgaccat tctcagcgcc ctgatttgcc gtagcgattt agatgcaata acggcgttgc
     8161 gaaaccatct ggataccgcc aaacaatcaa tgattcgttc catcaggcaa catcaccgat
     8221 aaattaagta cagattaaaa acattgattc ttggtttata tcactatgtt gtattaatgc
     8281 agtattaatc caggcgattg ttctgcccat aaggaaatac atagggaata aacgatgctg
     8341 gaagtattac tctcagccat catttaactc cctattactg acagcgataa aattatcggc
     8401 tatccatgat taacgtttcc agcacatcta attcactgac tatttttgcc agtgtttcat
     8461 cactgacttc ttgccttgcg cgcatctggt aaatagccgc gcgtttggca tttaacgccg
     8521 ccagattaaa tgttctttcc agcttcgcac gctgcatatg cagggaatta tcattaggcg
     8581 acatatcctc ttcactatgt cgcatataac cgataactct ggcccccgtt tccataatca
     8641 actcgttatc gaggttttct gttctggcgc gcagcaaacg ttgctcggtc ttttgcacac
     8701 tcagtatggc cattttagcc ataaacagat cggcagcttc ctcttctttt tgtcggttat
     8761 cgctgaaaat ccccacattt ttccccagta ataacggcag aataataatg ccgcaaacca
     8821 gcgaaaagag gatcacacct gtggcgagaa ataccagcaa gtaacgcccg ggaaaaggtg
     8881 tgccatccgg cagcaataac gggatagaaa gcccccccgc aagcgtgata gctccgcgca
     8941 cccccgcaaa ggtcgccagc agcatatgac gcgttttata tccagcaaac agcatcggat
     9001 gtttgccggg tcgtttacgg ctatacagac gcatcagcca aagccagccc aggcgcacaa
     9061 ccattaacgc caggtagatc caaatgacat caaggagcag cgatccggtg tccaccagtg
     9121 gatcgttttc tgcacggatc agtgattccg aaataatgcc cggcagttgt aagcctaaaa
     9181 tcagaaacac catgccgtta aagacatatt caagcatcga ccagctactt ttagctttaa
     9241 ttctcaggcg aataggcgcg tggtggacta cgccaatgcg actggttgtt aaccccgcgg
     9301 cgacagcggc aagaatgcca gataagccaa aatgttccgc aaccagataa acaataaacg
     9361 gcaacagaag catcaataca atctgaatgg aaggttcatc gtgagcatat cggctgatca
     9421 acagcagccc tttaccatat aaccaggtaa cagcgatccc cacggcaata ccgcccaacg
     9481 caacgacaaa aaacgcaatg gttgcgccat gaacgctgaa ttccatcgtt cccatcgtca
     9541 ctgcaacagc aaatttcaac gccaccaggc cggaagcatc attcatcaac gcttcgcctt
     9601 cgagcacttc ctggatgttt ttgctgatac gccctttccc gacaatcccc agcaacgcca
     9661 cggcatcggt aggcgacaac acggcggcta atgcgaaagc agcgatgagc ggcacattcg
     9721 gtaagagtaa atgaatcaca tagccgacac caaaaatagt gattagtacc aacacaagtg
     9781 ccaggctgat aatttcccgg ctgtgcttga tcaattcttt aggttgtatt tttgaacttt
     9841 cagcaaacaa caggggtggt ataaacaata agagaaaaag ttcaggattg aattccacat
     9901 gcagaccaaa tataggctgg gccagtactg caccagcggc aatctggatt aaaggaagtg
     9961 gtatctggac aggagacaga cgtgtaagca cgctggttaa tgatactacc aacgtaagaa
    10021 ttaaaattgt taaaagtatt tccatata
//
LOCUS       DAZFJL010000161         9916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZFJL010000161 DAZFJL010000000
VERSION     DAZFJL010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.163"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(6..125)
                     /locus_tag="ACHJ8C_004088"
                     /pseudo
     CDS             complement(6..125)
                     /locus_tag="ACHJ8C_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            203..1297
                     /gene="ddlA"
                     /locus_tag="ACHJ8C_004089"
     CDS             203..1297
                     /gene="ddlA"
                     /locus_tag="ACHJ8C_004089"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO5553866.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYTIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1321..1533)
                     /gene="yaiZ"
                     /locus_tag="ACHJ8C_004090"
     CDS             complement(1321..1533)
                     /gene="yaiZ"
                     /locus_tag="ACHJ8C_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO5553867.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1793..2101
                     /gene="yaiY"
                     /locus_tag="ACHJ8C_004091"
     CDS             1793..2101
                     /gene="yaiY"
                     /locus_tag="ACHJ8C_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO5553868.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAIVALWLHWRHRRQ
                     "
     gene            complement(2160..3254)
                     /gene="yaiW"
                     /locus_tag="ACHJ8C_004092"
     CDS             complement(2160..3254)
                     /gene="yaiW"
                     /locus_tag="ACHJ8C_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO5553869.1"
                     /translation="MSRVNPLSSLSLLAVLILAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3267..4487)
                     /gene="sbmA"
                     /locus_tag="ACHJ8C_004093"
     CDS             complement(3267..4487)
                     /gene="sbmA"
                     /locus_tag="ACHJ8C_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO5553870.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4839..5996
                     /gene="ampH"
                     /locus_tag="ACHJ8C_004094"
     CDS             4839..5996
                     /gene="ampH"
                     /locus_tag="ACHJ8C_004094"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO5553871.1"
                     /translation="MKRSLLFSAVLYAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVIRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(5997..6620)
                     /gene="iprA"
                     /locus_tag="ACHJ8C_004095"
     CDS             complement(5997..6620)
                     /gene="iprA"
                     /locus_tag="ACHJ8C_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO5553872.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6706..9636)
                     /gene="ehaB"
                     /locus_tag="ACHJ8C_004096"
     CDS             complement(6706..9636)
                     /gene="ehaB"
                     /locus_tag="ACHJ8C_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFO5553873.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECIATDCSNRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADSHVVDVFNIGTAITLDQEVDLSISNNSHVAGITLTQSYEWEDIDDNTVSTGVNSG
                     EVFNNTINVANSTVTSGSWSDEGIEGWFGNTGNASDYNGIDWNANDVALAIIAHPNAD
                     NAMQTTATFDNSTLMGDVFFSSNFDENFFPNGADSYRDADGDLDTNGWDGTDRLDLTL
                     NNGSKWVGAAMSAHQVVTAYGNIDYNAGTEKTATLIDITANSLWPSSTVGVENDDSAY
                     NEYEHIIGNEVYQSGLFNVTLNTGSQWDTTKTSLIDTLSINSGSTVNVADSTLISDSI
                     SLTGLSALNINEDGHVATDSLTVDNSTVTISDEVSAGWAVGDAALYANNIKVTNDGIL
                     DVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNIESNRFVLNADLVNDRTNDTTKANY
                     GYGTIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNAGDRVAAATGNYKVRIDNATGA
                     GSVADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANM
                     ALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVNYFGGNFNGDNGTINYDQDV
                     NGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVF
                     VDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVS
                     GLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDS
                     NNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGV
                     KYTW"
BASE COUNT         2566 a         2464 c         2359 g         2527 t
ORIGIN      
        1 ggtcatttaa gccaggaata aattagcttc ccgttcatca tttttgtgat cttgcacaca
       61 tctttgagat aacgcacttt cttcaccagt acgttgctcg ctttgtcagc agaagattgc
      121 aacattctgt cagaaggccg cgaatccccc gttttgtcgt ggtaatgtat gcactttgtg
      181 ttagacggga taggttttta agatggaaaa actgcgggta ggaatcgttt ttggtggtaa
      241 atcagcggaa catgaagtgt ctctgcaatc ggcaaaaaac attgtcgatg ccattgataa
      301 aagtcgcttc gatgttgtgc tgctgggcat tgataaacaa gggcaatggc acgtcagcga
      361 tgccagcaat tatctgctaa atgcagacga tcctgcccat attgcgttgc gcccttcggc
      421 gaccagcctt gcgcaggtgc caggtaaaca tgagcatcag cttatcgacg cacaaaacgg
      481 tcagccgttg ccgacggtgg atgtcatttt cccgattgtc cacggtacgc tgggcgaaga
      541 tggttccttg cagggaatgc tgcgggtcgc caatttaccg tttgtaggtt ctgatgttct
      601 ggcttcagcg gcctgtatgg ataaagatgt caccaaacgt ctgctgcgcg atgccgggct
      661 gaacattgcg ccatttatta ccctgacgcg cgctaatcgt cacaacatca gttttgccga
      721 agtggagtct aaactggggt taccgctgtt tgtaaaaccg gctaatcagg gctcttctgt
      781 tggtgtcagc aaagtaacca gtgaagaaca gtacacaatt gccgtcgatc tggcgttcga
      841 gttcgatcat aaagtgatcg ttgagcaagg gatcaaaggt cgtgaaatcg aatgcgcagt
      901 tcttggcaac gataacccgc aagcaagcac ctgtggcgag atcgtactca ccagcgattt
      961 ctatgcctac gacaccaagt acattgacga agatggcgcg aaagtggtag ttccggcagc
     1021 cattgcgcca gaaatcaacg ataagatccg ggcgattgcc gttcaggctt atcagacgtt
     1081 gggatgcgca ggcatggcgc gtgtagacgt gtttttaacc ccagagaacg aagtggtgat
     1141 caacgagatc aacaccctgc ctggcttcac caacatcagt atgtatccga agctgtggca
     1201 agccagcggt ctgggttaca ccgacctgat cacgcgtttg attgagctgg cgctggagcg
     1261 tcacgctgcg gataacgcac tgaaaaccac aatgtaatat tcaaacgccc ggtaatattc
     1321 ttattgggcg ttttcagttt cttcatcgcg acgacgaata atcaaccccg ccagccagaa
     1381 actaatcacc cacgtcacca gaccgacggc ataggtctgc caaccttttg cttcaaatcc
     1441 cagtaacccc accacgccat taagaatgaa tataaggcca atggcgaacg catagtagtg
     1501 ccagtcacgg cggattttta caggcagatt catggcagct ccgttaacga aaaacaacat
     1561 ccttgcatat tctgtaacaa ctgtctgtgg tgtagacaac actttcgcaa ttgttaataa
     1621 atttaactta aggttgctat tttgtgactc acaacgaaga ttcacgcttc gggaatattc
     1681 ccaacatcaa tttaccatga ttctgatatt gacaatctaa atgacctgtc agacttgcca
     1741 taagccgctg gtatcacacc agaagagaag cagatgactt ccggaggttt ttatggctga
     1801 tttcaccctg tcaaaatcgc tgtttagcgg aaaatatcgc aatgcatctt caacgcctgg
     1861 caatattgcc tatgcgttgt ttgtgctgtt ttgcttttgg gctggggcgc aattgctgaa
     1921 cctgctagtg catgcgcccg gcgtctatga gcgtttaatg caggtccagg aaacaggtcg
     1981 cccacgggtg gaaattggtt taggtgtcgg caccattttt gggctgatcc cgtttttagt
     2041 aggctgcctc atttttgcaa tagtggcgct atggctgcac tggcgacatc gccgccagta
     2101 agatttacgt tgccagatgc ggcctaaaac gccttatctg gcgtgacatt cctgtttgat
     2161 cactgtttca tacaacgcgc ccgccgttcg tctacgcgct tcgcaaacca ggccgtagtc
     2221 agattgcggg tgattttcgg gctttccagt tgaatgccag gcaacatttc gcggggtagt
     2281 gatttaccgg ttttcgcttc ggcaagttga taaactttct tgtacagcgc cgtctcctca
     2341 aaagagaagc tgtccccttt ctctaactga cggcgaattt cgctgtcgct catccccagt
     2401 tttcccgcca gtttgcgcgt tgccagttcg gttttcccgg gctctttact gtcgtaacga
     2461 attaaatcac cgtccagtgc cagcttcacg ccgctggcct tactgaccgc gttctgaaac
     2521 gcggcattac ggctggcgta ccagccagcg ttaaaatcag caaaacggta tatcggcgcg
     2581 ctatagctgg cgggataatt cagtaaatgg taagtaccaa accacaaccc gccgcggcga
     2641 ctgaagactt cctgacggac tgtaccgtcc attttccacg gatacccttt ggtatgctgt
     2701 tcggcaaaag caatactgac ctgcatcggc ccaccggtgc gcaccggatt gagcgaacca
     2761 aacagcgtct gccccattgg caccatgctg atcagatcgt caaaaatcgc gctcaattgc
     2821 ttttctgtac gcaccgaatc taaccgttcg ctatagcttt tgccgtttgg cgatttgatt
     2881 tttagcgcgg tatgcaccag aaaagcagga atgtgcatcc gttcagcacg acggtcaatc
     2941 tcttgccagg cgatcttgct taagcccgga acggccggat ctgcctgata atttgactcc
     3001 tgctgcgcca ccgccagcac cgagcagacg ttttccagcg tcggtgccag cccctgactt
     3061 tcaaaggtgg tggcgagatc tttcgcccag gcatcgcggt ctttcacgct cgcaggcatc
     3121 ttctgccgca cgacactcgc cacatctatc gctttttcgc cctttttcaa cggttgcgga
     3181 gcctgactac tacaccccgc taaaatcaga accgccaaca atgacagcga agatagagga
     3241 ttgacgcgcg acattacttc tcctttttag ctcaaggtat gggttacttc ctgaattttg
     3301 tcaccatcca gctcatgttc aaagctgcgc agacgtttgt agatagacat caactcaacc
     3361 agtgtggtcc atgagttaat caggtactgg aaagcaccgc gaacctgacc aaaaacgtta
     3421 gtaatctgcg tcatcaggcc gagtgtaatc gtaccggcaa caattgacgg aaacagcaag
     3481 aacaaaccga aaacgttatc gacctgcaaa tagagaatgc gggcgatatt gaaatacata
     3541 tagtgaaaat agaggcggaa atagtttttc cgtacggcgc taaacagctc gcgtaccgta
     3601 ggcggcgtcg cgcgcgtggc atcgtcttca ccataaacca gctctttacg gtaggcagcc
     3661 tctacacgct ggtttttaaa ctccagcccc ggcagtttga tccctaccac tgccagtaat
     3721 ccggtcccca tcagcgacca gacgatagcg gcaatcacca gaccatacgg aatgtgcccg
     3781 acaatcggca gctccggcac atgtgcggag agcgttacca gcaccggcag gaaggcgatc
     3841 aacgtcatga tagcgttgat aaagctgacg cccatattct ccagcgttga agcaaaacgc
     3901 atggtgtctt cctgcacacg ctgcgcggcc ccttcgatat gacgcagttg ttgccagttc
     3961 gccatgtaat attcgttcat cgccgtacgc cagcggaaca cgtagtgact gacaaagaag
     4021 ttgttcagca cactgatcac caccgcgatc agcgcaatcc ccagaaagac gcccacttcg
     4081 cggtaaaatt gttcgatggt gactttatgc ggcgaactta gcgccgtttg aatcagatca
     4141 tagaacggcg cataccaggc gttgacggca accccgactt ccaccaaaaa ccaggtgacg
     4201 aagataatca gcgcagtacc gagtattgac cagtattgcc aacggtgcgg gctatagata
     4261 aaccagaaca atgcaaaaag tcctacgcaa acaatgtagt aagcgtaaaa aatcaggaaa
     4321 tccaacgacc agaaacgcgc ggcgctaatc gggatctgcc cggaagcgcc ggtgatacgc
     4381 gccacccaat cacccccacc ggcttgccag aagataacgg caatcaatgc ccaaacaaag
     4441 gccgagagaa aaaacgttcc cggctttggg aaaaaagact taaacatatg cactcctgct
     4501 aacttcttat cgttttaaca actgctgtgt attaaccgca tgaccgctac aaagcgtcag
     4561 ccaaatgaaa ctgcccattg tgacccatag caaggcgctt agttcgccag cctgaccata
     4621 agaattgttt cgacgggttt cacaaaccaa agattgttta cataagcgga ttaacacgcg
     4681 caggatcaca aatgcaggca ttggtgagca ggaaaaagat cgtgtttacg cgccgttacg
     4741 tttttaacac tattattgca ctaattcgcc ctttgcaatc catcaatgag tagtataaat
     4801 acgctcagtt accttcattc aatctatgga caccaccgtt gaaacgtagt ctgctttttt
     4861 ctgccgtgct gtatgcggcg tcattgacct ccgtccatgc ggctcagccg atcactgaac
     4921 cggagtttgc ctctgatatt gtcgatcgtt atgccgatca tattttttac ggcagcggtg
     4981 ccacggggat ggcgctggtt gttatcgacg gtaaccagcg cgtctttcgc agttatggcg
     5041 aaacgcgacc tggtaataac gttcgcccgc agctggattc cgtcatacgt attgcttccc
     5101 tcaccaagtt aatgaccagt gagatgctgg tgaaattgct cgaccagggg accgtgaagt
     5161 taaacgatcc gctaagtaaa tatgctccgc caggcgcacg cgtaccgacc tacaatggga
     5221 cgcccattac gctggtgaat ctggcaaccc ataccagcgc cctgccccgt gaacagcccg
     5281 gtggcgcggc acatcgtccg gtatttgtct ggccaacgcg cgagcaacgc tggaaatacc
     5341 tttccactgc caaactaaaa gccgcgccgg gcagccaggc cgcgtactcc aacctcgcat
     5401 tcgatctgct ggcagatgca ctggcgaatg cgtccggcaa gccttatacc cagttgtttg
     5461 aagagcagat tacccgtccg ttggggatga aagacaccac ctacaccccc tcaccggatc
     5521 agtgccgccg tttaatggtt gctgagcgcg gtgccagtcc gtgcaataac acgctggcgg
     5581 caattggcag cggcggcgtc tattccacgc caggcgatat gatgcgctgg atgcagcagt
     5641 atttgtcatc tgatttttat cagcgcagta accaggctga tcgcatgcag acgttgattt
     5701 atcagcgcgc gcagtttaca aaagtgattg gcatggatgt ccccggcaaa gcggatgcgc
     5761 ttggtttagg ctgggtatat atggccccga aagagggtcg tccggggatt attcagaaga
     5821 caggtggtgg tggcggtttc attacctata tggcgatgat cccgcagaag aatattggcg
     5881 cgtttgtggt ggtcactcgt tcgccgttga cgcgctttaa aaatatgagc gatggcatca
     5941 atgacctggt gaccgagcta agcgggaata aaccgttggt tatccccgca tcctgattaa
     6001 tactctgaag gcaaacggtt gatagcgacc agtttgcctt tattcatttc gatatagccg
     6061 cctttacgca aagcagcgag aacttccgca acgacagaac gcgatatacg tgtacgttga
     6121 tggatataat tcatcacgcc aatacgtgat cgcaattctt cattccagtc aatcattgat
     6181 aataacgtcg cgcggatttg ttcgtaggaa ttatgcccaa tgagctgaac gtcgcgtaat
     6241 tccagaattc tattttgcca ggctaaccag taaaaagcgt ctcgccagag ttgattttgt
     6301 tcaataagag taatggtttg tttggctggc aaatgatatc ccgtacagtt tccttctgat
     6361 attaatttgt atggtatatc gtttttcatt aaaccatcag ccagccccat aatataagga
     6421 gcctgtgtaa taccgataag tacgttttct tctctacgca aagagataac gccctctaaa
     6481 ataacgaaag tatcttcgct attgacatca ctggaaaata taacttgctt ttcattatta
     6541 aactcgaagc gcgtaccgta tctggacaaa catttatcga gcttaccaaa ttcctgaaga
     6601 ggtttaacta cagataacat ttgcgcgtcc tttgcagtaa tgcccgtcaa atccttgacg
     6661 gacattattt agattaaatt acctatttct tcggagtgaa gaatattacc aggtatattt
     6721 aacacccaca ttcgcggacc agtcttgatc tacgtcacca ccaccgaggt agttagcatc
     6781 ggtataggcg ctgaagttct tggtgaagct gaactgagta cccagaccaa cacgtaccgc
     6841 agacccttca gtaccgttat cgatggaatc accgttcaca tcgttatcgt tgttagagtc
     6901 gtcgtagacg taagccagtt tgaagtacgg agtcagagcc tggtcttcgc tgtaggtgaa
     6961 ggtataacct gcatctacac ccagttcata acgcatgctg tcgtaagact gaccatcaac
     7021 tttcatgtcg ttgctcagct ggtaatcatc accagactgg aacagaccag aaacgctgcc
     7081 gtaaggagtc acgtaacctg catcacccag tttgaagtcg taaccggctt tcaaaccaaa
     7141 gccccaagcg tcggagttgg tgctgccgtc aacgtaagta ccgttgctca tggttgcaga
     7201 caggtcgttg ttgaagtgag agtagctcaa gctaccgtca acaaagacgt tgttcgcgaa
     7261 gtgagcagaa gagtagatgt aggcagtctg gctgtcttga tccacctgac cagaacggtc
     7321 attcatatca cctttagcga agcctgcagc cgcaccgacg atccacttag cgttgttacc
     7381 gtcaattttg gtatcaacac cgaccatgat gccgttaaca tcctgatcat agttgatggt
     7441 gccgttgtcg ccgttgaagt taccaccgaa gtagtttacc catgcgccgc cgttatcagc
     7501 cagaccatga cgagagttgg tcagacgagt accaacggtg tcttgttcca ggttccagat
     7561 attggtgttc gcagacggga tgctcagcgc catgttagcg tagtcggtca gctccatctg
     7621 ttgcagaaca acggtgttac cacgctgctc agcctgatag gtgtatgcac ccaggtcagc
     7681 tttgttagca gcagagaagg ttgcgttgct gtttttgtcg ttaacgtaga tcagttcgtt
     7741 accgttgtag tcagcaacag aacctgcacc agttgcgttg tcgatacgaa ctttgtagtt
     7801 accggttgcc gcagctacac ggtcacccgc gttatcaact tcgttctgac cagcttcgat
     7861 agaagcagtg ttgtcgttat cgccgttacc attgatggtc aggtgaccat cagagttcat
     7921 cgcgatggtg ccgtagccgt agttagcttt agtggtatcg tttgtacgat cgttgaccag
     7981 gtcagcattc agcacgaagc ggttgctttc gatgttgaac acaccagcgt gaatgttacc
     8041 actggtatca gtagtgctgg tcaggttcag agtatcaacc tgcagagcat tcgccgcagt
     8101 gttacctaca tccagaatac cgtcgttagt cactttgatg ttgttggcat acagagccgc
     8161 atcacctaca gcccagccag cagaaacttc atcagaaatg gttacggtac tgttgtcgac
     8221 agtcagtgaa tcagttgcaa catgaccatc ttcgttgatg ttcagcgcag aaagaccagt
     8281 cagagagata gagtcagaaa tcagcgttga atcagcaaca ttaacagttg aaccactgtt
     8341 gatgctcaga gtatcaatca gagaagtttt ggtggtatcc cactgtgaac cggtgttcag
     8401 ggtcacattg aacagaccgc tctggtaaac ttcgttacca ataatatgct catattcgtt
     8461 gtatgctgaa tcatcgttct caacgccaac agtcgatgac ggccacaagc tgttcgcggt
     8521 gatatcgata agcgttgcag tcttttcggt accggcgttg taatcaatgt taccgtaagc
     8581 agtgacaacc tggtgtgcgg acattgccgc accgacccat ttgctgccat tgttcagggt
     8641 cagatccaga cgatcggtgc catcccaacc attagtgtcg agatcgccat ccgcgtcacg
     8701 gtaactgtca gcaccattcg ggaagaagtt ttcatcaaag ttgctggaga agaaaacgtc
     8761 gcccatcagg gtagaattat caaacgttgc agtcgtctgc atcgcgttgt cagcatttgg
     8821 atgtgcaata atcgctaatg ctacatcatt agcattccag tcaatgccgt tgtaatcgct
     8881 ggcattgcca gtgttaccaa accaaccttc tattccttca tcagaccatg aacctgaagt
     8941 gacagtagag ttcgcaacat tgatggtgtt gttaaacact tcgccgctgt ttacaccagt
     9001 gctgacagtg ttgtcgtcga tatcttccca ctcataactc tgagtcagcg taatacctgc
     9061 tacatgagag ttattagaga tggacagatc aacttcctga tccagagtga tggcagtacc
     9121 aatgttaaat acatctacaa catgagagtc agcggcatta ttgtaggtgc cgttatatgt
     9181 gtagtgttcg tagttgtcat cgatcgttga attatcaacg gtcagcgcca gacgatcatg
     9241 gtagtagtca tccgcacgat tagaacaatc agttgctatg cactcggaat aaatcatacc
     9301 gtgaatagtg ctgtttttga ttgtcaggct attggcgttg gtattagtag aaataccatc
     9361 gtccagatag taggtagaaa tcacgccgtt aacggtagcg ttattaatga ccggatagat
     9421 atcgccgttg taataactat cagcggcata gttattccag cctacataac catcatagta
     9481 aacgtcatca gcataggtgg cgtcgttgta gtgatggaaa gtattgtaag tttgaccaga
     9541 aatatcgttg gtcgctgcat tagcctgaga ggtgatagcc agagtgcaag ccaatgctaa
     9601 ttgtgatact acaagtttct ttttccagga gtgcatttgt catccctcct cagggacgta
     9661 ataagttgat ccatcaatta tcaatgcata gaaaacatat tatgtttcgc gtttacgatt
     9721 atgcggcgtt atagaggaat ggttcaatta ttctttcccc taagtccgat tgcggacata
     9781 tataaagcac gtgaacacac acaaagaaag aaataacaaa aacacaatat ttcaatacgt
     9841 taatgaaagc gttaatttta aaacatagac catggaaata taaaacaaca aaaacagaca
     9901 aataatgtat caataa
//
LOCUS       DAZFJL010000162         9801 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFJL010000162 DAZFJL010000000
VERSION     DAZFJL010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.164"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            91..807
                     /gene="nanC"
                     /locus_tag="ACHJ8C_004097"
     CDS             91..807
                     /gene="nanC"
                     /locus_tag="ACHJ8C_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO5553874.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFSIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            827..1933
                     /gene="nanM"
                     /locus_tag="ACHJ8C_004098"
     CDS             827..1933
                     /gene="nanM"
                     /locus_tag="ACHJ8C_004098"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO5553875.1"
                     /translation="MNKTITALTIIMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTTIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVASPDGVAGGFAGMSNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSADIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1998..2978
                     /gene="nanS"
                     /locus_tag="ACHJ8C_004099"
     CDS             1998..2978
                     /gene="nanS"
                     /locus_tag="ACHJ8C_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO5553876.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDGEDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPVEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWRER"
     gene            complement(2986..3090)
                     /locus_tag="ACHJ8C_004100"
                     /pseudo
     CDS             complement(2986..3090)
                     /locus_tag="ACHJ8C_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3190..3784
                     /locus_tag="ACHJ8C_004101"
                     /pseudo
     CDS             3190..3784
                     /locus_tag="ACHJ8C_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224862.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
     gene            complement(3803..4111)
                     /locus_tag="ACHJ8C_004102"
                     /pseudo
     CDS             complement(3803..4111)
                     /locus_tag="ACHJ8C_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            4114..4961
                     /locus_tag="ACHJ8C_004103"
                     /pseudo
     CDS             4114..4961
                     /locus_tag="ACHJ8C_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028172.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(5180..5296)
                     /locus_tag="ACHJ8C_004104"
                     /pseudo
     CDS             complement(5180..5296)
                     /locus_tag="ACHJ8C_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611456.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5556..6737
                     /locus_tag="ACHJ8C_004105"
     CDS             5556..6737
                     /locus_tag="ACHJ8C_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFO5553877.1"
                     /translation="MPQVSFITPLDQPSGSKRLLDILRTGLKDPRFTKLRIVTAYAKS
                     GPLLRLKSDIESWAASGNSLCAILGIDQQGTSKEALTIAADLCKELYITRENGITFHP
                     KIYLFDGPKHAKAIIGSNNLTVGGTETNFESSIILDLVLPEDDSIYKDLDTMWGNLLP
                     HSCVATKAFDQQLLDELIANGAVLSELDKQARAKLTGNYPTAPRTGLIRKPASPLPTS
                     IMKASKTQVAGIMGQPQSPQQVVTGLIIQIKPHHNGEIFLSKIAVLQNPSFFGWPFKG
                     STIPKKAGNPAYPQRTPDPVVNINVYGPATTPILTLSSYQLNTVYYSAKAEIRITASP
                     LVSQVPDYSIMVMMPGNSAEIDYEICIYRPDSPQYTSWLSACNQSMPSGGKKARKFGW
                     F"
     gene            complement(6751..9522)
                     /locus_tag="ACHJ8C_004106"
     CDS             complement(6751..9522)
                     /locus_tag="ACHJ8C_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFO5553878.1"
                     /translation="MPTQKAVNIANLTDMEVINNEPALHLRPVMEVAHELGAIDWSFT
                     EVDTRLPGHDIHPYPAKFIPQLPHGLISRLSGRGETVLDPFGGSGTTALEAVKLGRKA
                     ISIDANPLSALIGRVKTARLEPETLKALNAHHSVLLTHLEGGHLSPSSLINNYSSHAP
                     KIVNQEKWFADTAYAELCLIKAYINELDSTSQDIALVALSRIVIKASFQDSETRYKSV
                     PREVPMGETLSRYLREFTAVIKSVEKNEAATRYGLSQFLCDDIRVISKEKLPDGIADL
                     VVTSPPYGNATDYHLYHRFRLLWLGFDPIALGHVEIGSHLKHQRESSGFESYLADMEA
                     ALATMHRALKPGRYAALVIGDSVYDKKTYDPAEALYERADSLGFEACTIVDRAIHAVK
                     RSFSHAGRRATSEHILVLRRKVAPTLIQISPAPYKLWPYEAELRLRELGLKLGDADPS
                     LDIRVPSLEEDRSMYKRAAFSHSVQLEGGYVEPTWQAVLENGEAWRSTTRKDPKYVTH
                     GIHSYKGKFYPQLAKSLLNISGFGPGATVLDLFCGSGTTLLEGYLNGFRTFGCDMNPL
                     AAKISRAKLGVLELNPDTVREVVLSVQEFLASPPSNFPQNLDHVEESCREEILRWFSP
                     TIAFKLNWVLGLLRRISAGVMLDFLEVILSSIIREVSNQEPTDLRIRYRSEPLTDADV
                     LGLFLDKLEDQFNRIEKFWKIRSNAPQAFLPSVITEGDNRKPDTFMKLGLESGSVDLV
                     LTSPPYGTALPYIDTDRLSLLFIMGLKSSERKPVENGLIGSREISTVERRRLEQIELR
                     EVLPSGSQHFISTMQRELARDLSAGFRKRNMPALMVRYLLDMSASLGQAKRLLRSGGE
                     MMIVIGDNKTTINGKVMLIPCTDLIEEIACTQGMVIVERIDISVTTENFKHIKNAIVK
                     NVVLRLRKP"
     gene            complement(9641..>9801)
                     /locus_tag="ACHJ8C_004107"
     CDS             complement(9641..>9801)
                     /locus_tag="ACHJ8C_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO5553879.1"
                     /translation="SHQERSSVRAAYIHKAEHLGERRLMLQWWADYLDANREKGISPF
                     DFGKMKSH"
BASE COUNT         2866 a         2189 c         2134 g         2612 t
ORIGIN      
        1 aagcctggca aaaaatatta cacaacataa atgctaattg tttatgcggg ctttgtattg
       61 ctttctgtat cctacaaatg agtgaaatat atgaaaaagg ctaaaatact ttctggcgta
      121 ttattactgt gcttttcgtc accactaatt tcccaggctg cgacactgga cgtacgtggt
      181 ggatatcgta gtggaagcca cgcctatgag actcgactca aagtcagtga gggatggcaa
      241 aatggatggt gggcaagtat ggaaagtaat acctggaata ccattcatga taataaaaag
      301 gaaaatgccg cactcaatga tgttcaggtt gaagttaatt acgcgattaa acttgatgat
      361 caatggacgg tgcgcccggg aatgttaacg cattttagca gcaacggcac acgctacgga
      421 ccctacgtaa aactgtcctg ggacgcgaca aaagatctta attttagcat tcgctatcgt
      481 tacgactgga aagcttaccg acaacaagac ttatccggtg atatgtctcg tgataacgtt
      541 catcgttggg atggatatgt cacttaccat attaatagtg atttcacctt cgcatggcaa
      601 acgacgctat acagcaaaca gaacgattat cgctatgcaa accataagaa atgggcgacg
      661 gaaaatgcat ttgttctaca ataccatatg acgcccgata ttacgccata catagaatat
      721 gactaccttg accgtcaggg tgtttacaac ggcagagata atttatcgga aaacagttat
      781 cgcattggtg tgtcatttaa actgtagtag acaggagaca gtcacaatga ataaaacaat
      841 aacggcgctt actatcataa tggcttcatt tgccgcaaac gcgtctgtat taccggaaac
      901 tcctgtgcca tttaaaagtg gtaccggagc aattgataac gacactgtct acattggttt
      961 aggtagcgct ggtacggcat ggtacaagct ggatacacag gccaaagata aaaaatggac
     1021 agcgttagct gcattccctg gcggaccgag agatcaagca acctctgcat ttattgatgg
     1081 caatctgtat gtgtttggcg gcattggcaa aaacagcgag ggcttgactc aggtatttaa
     1141 tgacgtacac aaatacaacc ccaaaaccaa tagctgggtt aaattgatgt cgcacgcgcc
     1201 gatgggcatg gcgggccatg tgacttttgt acacaacggc aaggcttatg ttactggcgg
     1261 tgttaaccag aatatcttca atggctattt tgaagatctc aacgaagctg gaaaagattc
     1321 aaccactata gataaaatca acgcccacta ttttgacaaa aaagcagaag attatttctt
     1381 caataagttt ctgttgtctt ttgatccctc tacacagcaa tggagttacg ctggcgaatc
     1441 tccctggtac ggaacggctg gtgcggcggt tgtgaataaa ggtgataaaa cctggcttat
     1501 taatggcgaa gccaaaccag gattgcgaac ggatgccgta tttgaacttg atttcaccgg
     1561 taataattta aaatggaata agcttgctcc cgtcgcatca ccagatggcg tcgctggcgg
     1621 ttttgcaggc atgagcaatg actcacttat atttgccgga ggggccggat tcaaaggttc
     1681 acgagaaaat taccagaacg gtaagaacta tgcgcatgaa ggcctgaaaa aatcatatag
     1741 cgctgatatt catctttggc ataacgggaa atgggataaa tcgggcgaat tatcgcaagg
     1801 tcgggcctac ggagtatcat tgccctggaa taatagtcta ttgattattg gcggtgaaac
     1861 tgcgggtggc aaagcggtga cggattcagt tttaatttct gtgaaggata ataaagtcac
     1921 agtacaaaac taacgcttca gggccccagt aaaggggccc tgctatcaat ttgtcatttg
     1981 tattacgcga aaattatatg aacgcaataa tatcgcccga ttattactat gttcttaccg
     2041 ttgctggtca gtctaatgcc atggcgtatg gcgaaggact gccattaccg gacggggaag
     2101 atgcgcctca tcccagaatt aaacaattag caagatttgc gcatacgcat cccggaggcc
     2161 cgtcatgtca ctttaacgac attattccac tgactcactg cccacacgat gttcaggata
     2221 tgcagggtta tcaccatcct ctggcaacga atcatcaaac acagtacggc accgttggcc
     2281 aggcactgca tatagcacgg aaattactgc cctttattcc tgataatgca ggggttctca
     2341 tcgttccgtg ttgccgtggc ggatcggctt ttaccgcggg cagcgaaggg acatattcag
     2401 aacggcacgg agccagccat gatgcttgtc gttggggaac ggatactccg ctataccagg
     2461 atttagtgag cagaacgcga gccgcactgg caaaaaatcc gcagaacaaa ttcctcggcg
     2521 tatgctggat gcaaggcgaa tttgacttaa tgaccagtga ctacgcgtca catcctcaac
     2581 actttaatca tatggttgaa gcctttcgta gggatctaaa acaataccat tctcagctta
     2641 ataatattac tgacgcgccg tggttttgcg gcgataccac ctggtactgg aaagaaaatt
     2701 tccctcatgc gtatgaagct atttacggca attatcaaaa taatgtttta gccaatatta
     2761 ttttcgtcga cttccagcaa caaggtgaaa gaggactgac gaacgcgcct gttgaagacc
     2821 cggacgattt aagcacggga tattacggtt cagcataccg gtcaccggag aactggacga
     2881 cggcactgcg aagcagtcat ttcagcgcgg cagcccgtcg gggaattatt tctgacaggt
     2941 ttgtagaagc tattttgcag ttttggcgcg aaaggtgagc gttcattaat ttgatttatt
     3001 cgcatccatc aacgctttta actcctccgg cgtaaacggt ggtttgcgtc ggtgcaacat
     3061 cgcatggcaa ttagggtaca caggcagcaa aaaaacaaat gagatcaagg taatatatct
     3121 tcacttgcag gcttgaacag cagaaaattt gcgagcatga tcgcaattat ttcactgcca
     3181 gggatatcaa tggcttccag taaatcgcta cagcaggcaa ttgccaatat aaaaatctgg
     3241 cacaaaggtg aacagcgcgc gccgcataag ccattgttat tgctatacgt attagcggga
     3301 tacctgaatg gacatccacg ccttttcgat tatggctcgg aaatctacga acctctgcac
     3361 agtttactgg aacgttttgg gccacagcgt tcgcagtacc gacctgatat gccgttctgg
     3421 cgattacaag gcgacggatt ctggcaatta cacaatgccg ggctttgctc aacagcaggg
     3481 agcagccgac aaccaccagt gaaggaactg actgaatacc acgttgcagg cggtttcgat
     3541 gaacagcact acgccctggt aactggcaat aaaaaactca tcaatactct ggcgcaacag
     3601 atactcgaag cgcacttcac cgagagtatt caggaagaaa ttgctgacga gctgggtttt
     3661 gatctccagc aaatccgtaa acagcgcgac ccgttatttc gcaaaaatgt attacgcgct
     3721 tataactacc gtcgcgttgg aagcggcgca tataaagggc ggtccttgtg aaattcctaa
     3781 tggccttgta aatagacccg ttttagttcc atgcattttt tgagaacccg gccaaataat
     3841 ggtgaagtcg gtattcctcc ggtatcatat tgtttcgtga ttcatacggg cgttcacagt
     3901 tatattctga taaccatttc tccgtaattt cccgtacttc attcagcgtt ctgaacagat
     3961 aaaaatcgag tatttctgta cggtatgtcc tgttaaaacg ctcaatgaaa gagttctgca
     4021 tcggcttacc cggctggatg aactccagtt ttactgcgtg tatctctgcc cattcagcca
     4081 gtgccagaga gataaattcc ggaccattat ctttactggc agcgtgagtt tgagaaactg
     4141 ggtcataatg taaaagtcat cagcccccag tatgtaaaac cttttgtccg taggcaaaaa
     4201 atgacggtaa cgatgcgcag gcgatagctg tggctctgat gcaacctacg atgcaatttg
     4261 ttccaccgaa aagccctgaa cagcaggata ttcaggctct acaccgtgca agacagcgta
     4321 ttgtcaatca tcgtactgcg acagtctgtt aaatccgagg gctgctgctt gatcagggaa
     4381 taacaattgg tgcggcagtc tcaagagttc gccgtgccat tccgcttatc cttgaggatg
     4441 cagaaaacgg gttaagtgcc cgtatgcgca gaacggttgc agagctttat gagctcttta
     4501 acaatcttgg tcgccgtata aacttttttg ataaagaaat tgacgctgtg ttcaggcaat
     4561 cagaagcctg ccagcgcatt gccaaagtga aaggcattgg tccaaaaaca gccacggctg
     4621 ttgttgccgc gattggcaaa ggaactgaat ttaaaaatgg tcgtcacttt gccacatggc
     4681 ttggtctggt tccacgacag cactcgagtg gcaacaagca ggtactgatg aatatgacga
     4741 aaaaaggcga caagcatttg cgtacacttt ttattcatgg tgcccgcgct gtcgtcagag
     4801 ttgccatgaa taacaatgat ggtagcttgt atcagtgggt taaccagtta aaggaacggc
     4861 gcgggtttaa taaaacgacc gtggcggtca ctaacaagaa cgcgagaata atctggtcga
     4921 tgctgagaaa tgataccgag tatcaggcag ttggaagtta gtacccagcc agacgagttg
     4981 cagtgtactg aaaaatggtg acaggttgca cctgctcaat cggaacctga ctattatatt
     5041 ggcctcagag gccgtccagt tgttgaggcg attgtgtgcg gattacccat ttgggcacag
     5101 gttttcctga tgccggatag atgtaagcaa caacccaaaa ccagaatcag tgcttacaaa
     5161 acggaggtag tccatagatg tagtaggtgt cttcagagat accggcctcc cagcagatat
     5221 ctttaacggt tcgtccggct tcaactgact taatcacagc gatgatctga tgctcagtaa
     5281 aacgggcttt acgcatagcg atctccttcg ttggtagatt gattatgccg gatgatctct
     5341 aaatatgaat ggcatgatta tgcgggatac ttacatgtcc agctataggg ctggatcgtc
     5401 taccgcggcg gggttagccc aactatgcaa tcgggatcac agttacgatt taacttacta
     5461 aaaagtaata gaaatcatta tgtaaaaagt agatactcaa cctaatcagc cagttttcat
     5521 agagcaaaaa tcaattaagg atagtaaaag atagcatgcc acaagtaagt tttattacac
     5581 cgttagatca gcctagcgga tctaaacgct tattggatat tttgcgtacg ggtctaaaag
     5641 atccgcgttt caccaaatta cgcatcgtca ctgcatacgc gaaatcaggc cctttactac
     5701 gtcttaagtc cgatatcgag agctgggcag cttctggaaa ctctctctgc gctatcttag
     5761 gaattgacca acaaggtaca agcaaagaag cgttaacaat tgcagccgat ttatgtaaag
     5821 agctttatat tactcgtgaa aatgggatta cttttcatcc aaaaatatat ttatttgatg
     5881 gtccaaaaca tgcgaaagca attatcggct ctaataacct tacggtcggt ggtactgaaa
     5941 ctaattttga aagttcgata attttagatc tagtgttgcc tgaagatgat agtatctata
     6001 aagatctaga cacaatgtgg ggcaatttat tacctcactc atgcgttgcg acaaaagcat
     6061 ttgatcagca attattagat gaattgatag ccaatggtgc tgttctatct gaattagata
     6121 aacaagcacg cgccaagttg acagggaact atccgacggc tccgagaact ggactgatac
     6181 gcaaaccagc aagcccactc cccacatcca taatgaaagc gtctaaaaca caagtggcag
     6241 gaattatggg gcaaccccaa tctccacaac aagttgttac agggctaatt atacagatta
     6301 agcctcatca taatggtgag atattcttaa gtaaaattgc agtactacaa aatccaagct
     6361 tctttggctg gccattcaag ggatcgacaa tacctaagaa agcgggtaac cctgcatatc
     6421 cacagagaac acccgaccca gtagtgaaca taaatgtata tggccccgct acgacaccaa
     6481 tattaactct ttcaagttat caacttaata cggtgtatta ttcagcaaaa gcggaaatta
     6541 ggatcacggc atcgccattg gtatctcaag taccagacta ttcaataatg gtaatgatgc
     6601 cgggtaacag tgccgaaatt gattatgaaa tttgtatcta tcgaccggat agcccgcaat
     6661 atacgtcatg gttatccgct tgtaaccagt caatgcctag tggcggtaaa aaagcaagaa
     6721 agtttggctg gttttaaacc agcctcacac ttatggtttc ctaagtctta gaaccacatt
     6781 tttgactata gcatttttaa tatgcttaaa attttcagta gtaacagata tatcgattct
     6841 ctctactatc accatccctt gtgtacaagc tatttcctca atcaagtctg tacaagggat
     6901 tagcataact ttgccgttaa ttgtcgtttt attatctccg atgacaatca tcatttctcc
     6961 gcctgaccgc aataatcgtt tggcttggcc aagggatgca gacatgtcaa gaagatatcg
     7021 caccattagt gctggcatat tacgttttct aaatcctgca gataaatcac gtgcgagctc
     7081 cctctgcatt gttgaaataa aatgttgtga acctgaaggt agtacctctc tgagctcaat
     7141 ttgctctaat cgtcggcgtt caaccgtact tatttcgcga cttccaatta gcccattttc
     7201 aacgggtttt ctttcagagc tctttaaacc cattatgaaa agtagagata gtcgatccgt
     7261 atcgatatat ggtaaagcgg ttccataagg cggactagtt aacaccagat ccacgctacc
     7321 agactcaaga cctaatttca taaatgtatc gggctttcta ttatccccct ctgtaattac
     7381 ggaagggagg aaggcctgag gagcattact tcttatcttc cagaattttt ctatccgatt
     7441 gaattgatcc tctaatttat ccagaaagag accaagcaca tcagcatcag ttaacggctc
     7501 agatcgatag cgaatacgga gatctgtagg ttcttgatta gaaacctcac gaataatgct
     7561 gctcaaaatt acttcgagaa aatctaacat tactccagca ctaattcttc gcagaagccc
     7621 caggacccag tttaacttga aagctatcgt tggtgaaaac cagcgcaaaa tctcttcacg
     7681 gcaactctct tcaacatgat caaggttctg cggaaaattt gaaggtggac tcgcaagaaa
     7741 ctcctgaaca cttaacacaa cctcacgtac agtatctggg tttagctcta agaccccgag
     7801 cttcgctcgt gatatcttgg ctgctagcgg attcatatca cagccgaagg tacgaaagcc
     7861 attcaaatat ccttccaaca aggtagttcc actaccacaa aataaatcca acactgtagc
     7921 gccaggacca aacccgctga tatttaataa acttttcgct aactgtggat aaaatttccc
     7981 tttatatgaa tggataccat gagtgacata tttaggatcc ttccgtgtag ttgagcgcca
     8041 cgcttcacca ttctccagaa cagcttgcca tgtaggctca acatatccgc cttccagttg
     8101 aacactatgt gaaaaagccg cacgcttgta catactccga tcttcttcaa gtgaaggaac
     8161 acgaatgtct aaagaaggat ctgcatcacc aagtttaagg cccaactctc tgagccttaa
     8221 ctctgcctcg tatggccata gcttatatgg cgctggagat atttgaatga gcgttggagc
     8281 gactttacgt cgtaaaacca atatatgttc agaagtcgca cgcctacccg catgtgaaaa
     8341 tgatctcttc acggcgtgaa tagctctatc tacaatagta caggcttcaa aacctaaact
     8401 atcagctcgc tcataaaggg cttctgctgg gtcatatgtc tttttatcat atacagaatc
     8461 tccaattacg agcgcagcat atcgacccgg ctttaaagca cgatgcatag ttgctaaagc
     8521 tgcttccata tctgcaagat agctttcaaa gccactcgat tctctctgat gttttaaatg
     8581 ggaaccaatt tcgacatgcc ctaatgcaat aggatcaaaa cccagccata ataaacgaaa
     8641 acggtggtaa aggtgatagt ctgttgcatt tccatatgga ggtgaagtca cgactagatc
     8701 agcgatccca tcaggaagtt tttctttact aatcactcga atatcatcac ataggaattg
     8761 tgatagtcca taccttgttg ccgcctcatt cttttccaca gacttaatta ctgcagtaaa
     8821 ttctcttaaa tacctgctta acgtttcacc catgggcact tcacgtggaa cgcttttata
     8881 gcgtgtttct gaatcttgaa aagaggcttt aattactatc ctagaaagcg caactagggc
     8941 tatatcttga ctggtgctat ccagctcatt tatataagcc ttaattaagc acaactctgc
     9001 gtatgcagta tccgcaaacc atttctcctg attaacgatc tttggtgcat gagaagaata
     9061 attattaatc aaagatgaag gtgagagatg ccccccctca agatgagtaa gtagcactga
     9121 atgatgggca ttcagtgctt taagcgtttc aggttcaagt ctggcagttt taactcgacc
     9181 aatgagggca gaaagtggat tagcatcaat actgattgcc tttcgtccta gcttcacggc
     9241 ttcaagtgca gtagttccgc tcccaccaaa tggatcaagc acagtttccc cgcgtccgct
     9301 cagacgtgag attaaaccat gcggaagttg cgggatgaac ttagcgggat atggatgtat
     9361 gtcatgtccc ggcaggcgag tatccacttc agtgaagctc caatcaatgg cccccagttc
     9421 atgagcaacc tccataactg gtctcagatg aagtgctggt tcattattta tgacttccat
     9481 atccgtcaaa ttagcaatgt ttacagcttt ctgtgtaggc ataaatgatg actctcaaat
     9541 gttaattctg ggattttatc aaaatcccag aattgattca attatacaat gatctattgc
     9601 catagcgtga tagctgcaag tgctgctgtt tccctaaaaa ttagtggctc ttcattttcc
     9661 cgaaatcaaa aggactaatg cccttctccc gattcgcatc cagataatca gcccaccact
     9721 gcagcatcaa cctgcgctcc cccagatgct ccgctttatg gatgtaggca gccctaacag
     9781 agctacgctc ctgatggctc a
//
LOCUS       DAZFJL010000163         9754 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFJL010000163 DAZFJL010000000
VERSION     DAZFJL010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.165"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            129..1286
                     /gene="acrE"
                     /locus_tag="ACHJ8C_004108"
     CDS             129..1286
                     /gene="acrE"
                     /locus_tag="ACHJ8C_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO5553880.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLSRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADATVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNNKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1298..4402
                     /gene="acrF"
                     /locus_tag="ACHJ8C_004109"
     CDS             1298..4402
                     /gene="acrF"
                     /locus_tag="ACHJ8C_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFO5553881.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIQEVVKTLFEAIILVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            4655..4876
                     /locus_tag="ACHJ8C_004110"
     CDS             4655..4876
                     /locus_tag="ACHJ8C_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553882.1"
                     /translation="MKRLIPVTLLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            5307..6332
                     /locus_tag="ACHJ8C_004111"
     CDS             5307..6332
                     /locus_tag="ACHJ8C_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5553883.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAFNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            6400..7581
                     /gene="yhdX"
                     /locus_tag="ACHJ8C_004112"
     CDS             6400..7581
                     /gene="yhdX"
                     /locus_tag="ACHJ8C_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO5553884.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIIGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     FAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            7591..8694
                     /gene="yhdY"
                     /locus_tag="ACHJ8C_004113"
     CDS             7591..8694
                     /gene="yhdY"
                     /locus_tag="ACHJ8C_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO5553885.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSLSNSLLTIGCIWL
                     MWELLPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPYRGRYIAVWAVIYPLIVWWLMYGGFLGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            8702..9460
                     /gene="yhdZ"
                     /locus_tag="ACHJ8C_004114"
     CDS             8702..9460
                     /gene="yhdZ"
                     /locus_tag="ACHJ8C_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO5553886.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAQKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPNEFFAHPKSERTRAFLSQVIH"
     gene            complement(9523..9638)
                     /gene="rrf"
                     /locus_tag="ACHJ8C_004115"
     rRNA            complement(9523..9638)
                     /gene="rrf"
                     /locus_tag="ACHJ8C_004115"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(9678..9753)
                     /locus_tag="ACHJ8C_004116"
     tRNA            complement(9678..9753)
                     /locus_tag="ACHJ8C_004116"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9718..9720),aa:Thr,seq:ggt)
BASE COUNT         2317 a         2345 c         2513 g         2579 t
ORIGIN      
        1 tattcataac tattccttac atcgacgaat gataatttgt aggatagcga actgtatttt
       61 tctttctgcg agttaacgcg ttgccttttt gggtaaataa cgcgcttttg gttttttgag
      121 gaatagtaat gacgaaacat gccaggtttt tcctcctgcc ctcctttatt ctgatctccg
      181 cggctttaat cgccggttgt aacgataagg gagaagagaa agctcacgtc ggtgaaccgc
      241 aggttaccgt tcatattgta aaaacggccc cgttagaagt taagactgaa ttaccaggcc
      301 gcaccaatgc ttaccgtata gccgaagttc gcccacaggt tagcgggatc gtactgagtc
      361 gcaatttcac tgaaggcagc gatgtgcaag caggccagtc tctgtaccag atcgatcccg
      421 ctacctatca ggcaagttat gacagcgcga aaggcgaact ggcgaaaagt gaagccgccg
      481 ccgccatcgc gcatttgacg gtaaaacgtt acgttccgct cgtgggtacg aaatacatca
      541 gtcagcagga gtacgaccag gccattgctg atgctcgcca ggccgatgcc accgtgattg
      601 ccgcaaaagc cacagttgaa agcgctcgca tcaatctcgc ttataccaaa gtgactgcgc
      661 ctattagcgg acgtatcggc aaatcgactg tgaccgaagg cgctcttgtc actaatgggc
      721 aaacgactga actggcgact gtccagcagc tcgatcctat ctacgttgat gtgacccaat
      781 ccagcaacga ttttatgagg ctgaagcaat ccgtagagca aggaaatttg cataaggaaa
      841 acgccaccag caacgtagag ttggtcatgg agaacggtca aacctatccc ctgaaaggta
      901 cgctgcaatt ctccgatgtg accgttgatg aaagcaccgg ctccataacc ctacgtgctg
      961 tcttccctaa cccgcaacat acgcttttac cgggtatgtt tgtgcgcgca cggattgatg
     1021 aaggcgtcca acctgacgcc attcttatcc cgcaacaagg cgttagccgc acaccacgtg
     1081 gtgatgcaac cgtgctgatt gttaacaata aaagtcaggt tgaagcgcgc cctgtcgttg
     1141 ccagtcaggc gattggcgat aaatggttga ttagcgaagg tctgaaatct ggcgatcaag
     1201 tcattgtcag cggcctgcaa aaagcgcgtc cgggagagca ggttaaagcc actaccgata
     1261 cccccgcaga tactgcatcg aagtaaggca atctgacatg gcaaactttt ttattcgacg
     1321 accgatattt gcatgggtgc tggccattat tctgatgatg gcgggcgcac tggcgatcct
     1381 acaattgccc gtcgcccagt atccaaccat tgcgccgcct gcggtttctg tttcggcaaa
     1441 ctatccgggt gctgatgcgc agaccgtgca ggatacagtg acgcaggtta tcgaacagaa
     1501 tatgaacggt atcgataacc tgatgtatat gtcctccacc agcgattccg ccggtagcgt
     1561 gacaattacc cttaccttcc agtccgggac cgatcctgat atcgcgcaag tacaggtgca
     1621 gaacaaactc cagctcgcca cgccgttgct gccgcaggag gttcagcagc aggggatcag
     1681 tgttgaaaag tccagtagca gctatttgat ggtggcgggc tttgtctctg ataaccctgg
     1741 caccacacag gacgatatct cggactatgt ggcctctaac gttaaagata cgcttagccg
     1801 tctgaatggc gtcggtgacg tacagctttt cggcgcacag tatgcgatgc gtatctggct
     1861 ggacgccgat ctgctaaaca aatataaact gacaccggtt gatgtgatta accagttgaa
     1921 ggtacagaac gatcagatcg ctgccggaca gttgggcgga acgccagcgt taccagggca
     1981 acagttgaac gcctcgatta ttgctcagac gcggttaaaa aatccggaag aattcggcaa
     2041 agtgaccctg cgcgtaaaca gtgacggctc ggtggtgcgt ctgaaagatg tcgcacgggt
     2101 tgaacttggc ggtgaaaact ataacgttat cgctcgcatc aacggaaaac cggcggcggg
     2161 cctggggatt aagctggcaa ccggcgcgaa tgctctcgat accgcgaaag ccattaaggc
     2221 aaaactggcg gaattacagc cattcttccc gcagggaatg aaggttcttt acccttatga
     2281 caccacgcct ttcgtccagc tttctatcca ggaagtggtg aaaacgctgt tcgaagctat
     2341 tatcctggta ttcctggtga tgtatctgtt cttgcagaac atgcgggcaa cattgatacc
     2401 caccattgct gtacctgtcg tgttgttagg gacatttgcc atactcgccg cttttggtta
     2461 ctccatcaat acgctaacga tgtttgggat ggtgcttgcc atcgggctgc tcgtcgatga
     2521 tgcgatagtg gtggtggaga acgtcgagcg cgtgatgatg gaggataagc tcccgccaaa
     2581 agaagcgacg gaaaaatcaa tgtcgcaaat ccagggcgcg ctggtaggta tcgcgatggt
     2641 actgtcggcg gtatttattc cgatggcatt cttcggtggc tcaacagggg caatttatcg
     2701 acagttctct atcactattg tttcagcaat ggcgctttca gtactggttg cgctgattct
     2761 cacccccgcg ttatgtgcaa cattgctgaa accagtctct gccgagcatc acgaaaataa
     2821 gggggggttc ttcggctggt ttaacaccac tttcgatcat agcgttaacc actacaccaa
     2881 cagcgtcggc aaaatcctcg gttccacagg acgatattta ctgatctatg cgctgattgt
     2941 tgcaggaatg gtggtgttgt ttttacgtct tccgtcttcc ttcttacctg aagaggatca
     3001 gggtgtcttt ctgaccatga ttcagttacc cgctggcgcg acgcaagagc ggacgcaaaa
     3061 agtgttggat caagtgacgg attactatct gaagaacgag aaagcgaacg ttgaaagtgt
     3121 ctttacagtt aacggattta gcttcagcgg ccaggcacaa aacgccggta tggccttcgt
     3181 cagtctgaaa ccgtgggaag agcgtagtgg tgacgaaaac agtgcggaag cagtaatcca
     3241 tcgtgccaaa atggaattgg gcaagatccg cgacggtttt gtcattccat tcaatatgcc
     3301 agccattgtt gaactgggta cggcaacggg tttcgacttt gagttaattg atcaggctgg
     3361 gctgggtcac gatgccctga cccaggcccg taaccagttg cttggcatgg cggcgcaaca
     3421 tcctgccagc ttagtcagcg tgcgtcctaa tggcctggaa gacaccgcgc agtttaaact
     3481 ggaagttgac caggaaaagg cgcaggcatt aggtgtttca ctttctgaca tcaatcagac
     3541 catttcaacg gcgttgggtg ggacttacgt taacgacttc atcgaccgtg gtcgcgtgaa
     3601 aaaggtgtat gttcaggcgg atgccaaatt ccgtatgctg ccagaagatg tcgataaact
     3661 ttatgtccgc agcgccaacg gcgaaatggt gccattctct gcctttacca cttcacattg
     3721 ggtgtatggc tctccgcgac tggaacgcta caacggtctg ccgtcaatgg agattcaggg
     3781 ggaagccgcg ccaggaacca gttccggcga tgctatggcg ttgatggaaa accttgcgtc
     3841 aaaattaccg gcgggcattg gttatgactg gacgggtatg tcatatcagg aacgtttatc
     3901 gggaaaccag gctcccgctc tggtagcaat ttcctttgtg gttgttttcc tgtgccttgc
     3961 tgcactctat gaaagctggt caattcctgt ctcggttatg ttggtcgtgc cgttagggat
     4021 tgtcggcgtg ctactggcgg cgacactctt taatcaaaaa aatgacgtct actttatggt
     4081 gggcttgcta acgacaattg gcttgtcggc taaaaacgct attttgattg ttgagttcgc
     4141 taaagatctc atggagaaag agggtaaagg tgttgttgaa gcgacactaa tggcagtacg
     4201 tatgcgtctg cgtcctatcc tgatgacctc tctcgccttt attctcggcg tattaccgct
     4261 ggctatcagt aacggggccg gcagtggcgc gcaaaacgca gtcggtatcg gggtaatggg
     4321 cggaatggtc tctgcaacgt tgctggcaat cttcttcgtg ccagtgttct ttgtggtgat
     4381 ccgtcgatgc tttaaaggat aaatcagaaa cataaaggcg ctttcaggcg ccttttttat
     4441 tttcagtgaa ccccataaaa attaataaga tattcttctt ctcactcttt aaaaactttc
     4501 tatagttccc gctcccttca ctatttttac aattcacata aattgtcatt attcattccg
     4561 caagatttat aacctccagt gataaaatgg cattcagctc gttaataaga gagttaactt
     4621 attaagcgtt agcgtttatt actgaggtaa caccatgaaa agattaattc ctgtgacatt
     4681 gctcaccgca ttgctggcgg gctgtgctca cgattcgccc tgcgttccgg tttatgacga
     4741 tcagggtcgt ctggttcaca ccaatacctg tatgaaaggc acgactcagg ataactggga
     4801 aacggctggg gctattgccg gcggggcagc agctgttgct ggcctgacga tggggatcat
     4861 cgctctgtcg aaataactaa acagggcgcg gagctccgcg ctctttttaa cgatacagta
     4921 tctttccata agataaaata ttgctatatt ctgctgggta attccctgct gatttagcat
     4981 gttatatcac gttatttcgc tgctttcgaa ataacgcatc aatttaactt tcacacatct
     5041 caccagaaat atactcacgc taacaatcat tattctgctt agtaaaaaac gcgtaaatat
     5101 tttgcgccaa aatgtggcgc atgtttcatt ttcgcaccat tgcggggcgc tgtttttatt
     5161 atttcctgtc tacactcagc ttattgcgac gcgaaatcgt gcgcaaaagc tggcactact
     5221 tttgcttata agaggatggc cacagacagg taaaagacgt ttcccaaacg tcctataacg
     5281 ataattttcg cctcacagga agcattatga aaaagatgat gatagccaca ctggctgccg
     5341 ccagcgtgct gcttgccgtt gcaaatcagg cgcatgctgg cgcgacgctt gatgccgttc
     5401 agaaaaaagg ttttgtgcaa tgcgggatca gtgatggatt acctgggttc tcttatgccg
     5461 atgctgacgg taagttttca ggtattgatg tagatgtttg tcgtggtgtt gccgctgctg
     5521 tattcggtga cgatacgaaa gtgaaatata ccccgctcac tgcaaaagaa cgcttcaccg
     5581 ctttacagtc aggggaggtg gatttgctct cccgtaatac gacctggact tcatctcgcg
     5641 atgccgggat gggaatggca tttactggcg tcacttatta cgacggcatt ggcttcctga
     5701 cgcacgataa agcggggcta aaaagcgcga aagaactgga tggcgctacc gtctgtattc
     5761 aggcggggac tgataccgaa ctcaacgtcg ccgattactt caaggcaaat aatatgaagt
     5821 acacgccagt aaccttcgat cgctcagacg aatcagcgaa ggctctggaa tctggccgct
     5881 gcgatactct ggcctcggat caatcacaac tgtatgccct gcgcatcaaa ttaagcaacc
     5941 cagctgaatg gattgtctta ccggaagtta tctctaaaga accacttggg ccggtagttc
     6001 gtcgtggcga tgatgaatgg ttctcgattg tacgctggac gcttttcgcc atgctgaatg
     6061 ctgaagagat gggcatcaat tcccagaacg tcgatgaaaa agcggctaat ccagcaacac
     6121 ctgatatggc acatctgctg ggtaaagaag gcgattacgg caaggatctg aagctggata
     6181 ataaatgggc ctttaacatc atcaaacagg tgggtaacta ctcggaaatt ttcgagcgta
     6241 acgtaggttc agaaagcccg ctgaaaatta aacgtgggca aaataatctc tggaataacg
     6301 gcggtattca gtacgcgccg cccgtgcgtt aagtcggtgt gaggtaacga gcgtcgctac
     6361 ggcggcgctc tcttcagcgt tatgctttcc gaggtttcta tgtctcatcg ccgctcaacc
     6421 gttaaaggct cactctcttt tgccaaccct acggttcgcg cctggttatt ccagatcctt
     6481 gccgttgttg ctgttgtcgg cattattggt tggttatttc acaacactgt aacgaatctc
     6541 agtaatcgtg gcataacatc aggatttgct tttcttgacc gcggggccgg ttttggcatc
     6601 gttcagcatt tgattgatta tcagcaaggc gacacttacg gacgcgtttt tattgtcggc
     6661 ctactcaata cgctactggt ttctgcattg tgtattgtgt tcgcttctgt tttgggcttc
     6721 tttatcggtc tggcgagact ttcggataac tggctgctac gaaagctttc cacaatttat
     6781 attgagatct tccgtaatat tcccccgctg ctgcaaatct tcttctggta ctttgccgtg
     6841 ttgcgcaatt tgcccggacc acgacaggca gtgagcgcgt ttgatctcgc ctttttgagc
     6901 aatcgtgggc tttatattcc gtcgccgcag ctgggagacg gatttattgc gtttatcctc
     6961 gctgttgtta tggctatagt cctttcagtt gggctattcc gctttaataa aacataccag
     7021 ataaagaccg gacagctgcg ccgcacctgg ccattcgccg ctgtgttgat cattggtttg
     7081 cctttactgg cgcaatggct ttttggcgca gcactacact gggatgtccc agcccttcga
     7141 ggctttaatt tccgcggcgg gatggtttta attcctgaac tggcagcctt aacgctggca
     7201 ctttcggttt atacctctgc atttatcgcc gagattatcc gcgcggggat ccaggcagtg
     7261 ccttatggtc aacatgaagc tgctcggtca ctgggattac ccaacccggt tacgctacgc
     7321 caggtcatta ttccccaggc actgcgggtg attattccac cgttaaccag ccagtatctc
     7381 aacatcgtca aaaactcctc tcttgccgcc gcgattggct atcccgatat ggtttcgctg
     7441 tttgctggca ccgtgctgaa ccagacgggg caagccatcg agacgatagc catgaccatg
     7501 tcggtctatc tgattatcag cctgactatc tcgctgctga tgaatatcta taaccgccgc
     7561 atcgcgatcg ttgaacgcta aggagtcatg atgacaaaag tattgctgtc tcatcccccg
     7621 cgcccggcga gccataactc aagccgcgcg atggtgtggg tgcgaaaaaa tctgttctcc
     7681 agcttgagca atagcctact gactattggc tgcatatggt tgatgtggga actgctccca
     7741 ccgttgctga actgggcatt tttgcaggct aactgggttg gctcaacgcg tgccgactgc
     7801 acaaaagccg gtgcctgttg ggtcttcatc cacgaacgat ttggtcagtt tatgtatggg
     7861 ctttacccac acgaccaacg ctggcgaatt aacctcgcat tactgattgg gcttgtgtcg
     7921 atcgcaccaa tgttctggaa aatactcccg tatcgcggtc gctatattgc ggtatgggcg
     7981 gtgatttacc cactgattgt ctggtggctg atgtatggcg ggtttcttgg tcttgagcgg
     8041 gttgaaaccc ggcaatgggg cgggctgacg ctaactttaa ttattgcatc agttgggatt
     8101 gcgggggcgc tgccgtgggg gatcttactg gcgttaggtc gccgctccca tatgccgatt
     8161 gtgcgtatct tatcggtcat ttttatcgag ttctggcgcg gcgtaccgct gattaccgtt
     8221 ctgtttatgt cttcggtcat gctgccgttg tttatggcag aaggcaccag tatcgacaaa
     8281 ttgatccgcg cgctggttgg cgtgatcctg tttcagtcag catatgttgc ggaagtcgtg
     8341 cgaggcggat tacaggcgct gcctaaaggg caatatgaag cggcagagtc gctggcgttg
     8401 ggttactgga aaacccaggg gctggttatt ctgccacagg cgttgaagct ggtgattcct
     8461 gggctggtaa ataccatcat cgcacttttc aaagatacca gcctggtgat catcatcggg
     8521 ttgttcgatc ttttcagtag cgttcagcag gcaaccgttg atcccgcctg gttgggtatg
     8581 tcgacggaag ggtatgtttt cgccgcactg atctactgga tcttctgttt cagcatgtca
     8641 cgttatagcc aacatctgga aaaacgtttt aacaccgggc gtacaccgca ttgaggacac
     8701 tatgagccaa attttactgc aacctgctaa cgcgatgatt acgctggaaa acgtcaataa
     8761 atggtatggg caattccatg ttttgaaaaa tataaattta accgtacaac cgggagaacg
     8821 gatcgttctg tgtggccctt ctggttccgg taaatcgaca accattcgtt gtattaatca
     8881 tctggaagaa catcaacagg gacggatcgt agtagatggc atcgaactta atgaagatat
     8941 ccgcaatatt gagcgcgtca ggcaggaagt gggaatggtc tttcagcatt tcaatctctt
     9001 ccctcatctg accgttctac agaactgtac cctggcaccg atttgggtac gcaagatgcc
     9061 taagaaagag gctgaagctc tggcgatgca ttaccttgag cgggtgagaa ttgccgaaca
     9121 tgcccagaag tttcccggac agatttcagg tggtcagcag caacgcgttg ccatagcacg
     9181 ttcactttgt atgaaaccaa agatcatgct atttgatgaa cctacgtcgg cgctcgatcc
     9241 ggagatggtg aaagaagtgc tggatacgat gattgggctg gcgcagtcgg gtatgacaat
     9301 gttgtgtgta acacatgaga tggggtttgc acgaaccgtc gctgaccggg tgatttttat
     9361 ggatcgtggg gaaatagtgg agcaagctgc acctaatgaa ttttttgcgc atcctaaatc
     9421 agagcgtacg agggcatttt tatcgcaggt aatccattaa ttgaatgtta gttcgaaaag
     9481 caaaaaggcc atcctttcgg atggcctttc gcttgatttg atgtctggca gttccctact
     9541 ctcgcatggg gagaccccac actaccatcg gcgctacggc gtttcacttc tgagttcggc
     9601 atggggtcag gtgggaccac cgcgctactg ccgccagaca aattcttttc taatctgccg
     9661 aactttaacc taaaaagtgg tgctgatacc cagagtcgaa ctggggacct cacccttacc
     9721 aagggtgcgc tctaccaact gagccatatc agca
//
LOCUS       DAZFJL010000164         9698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZFJL010000164 DAZFJL010000000
VERSION     DAZFJL010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.166"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(122..742)
                     /gene="sodA"
                     /locus_tag="ACHJ8C_004117"
     CDS             complement(122..742)
                     /gene="sodA"
                     /locus_tag="ACHJ8C_004117"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO5553887.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     NLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVQKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            1028..2062
                     /gene="rhaT"
                     /locus_tag="ACHJ8C_004118"
     CDS             1028..2062
                     /gene="rhaT"
                     /locus_tag="ACHJ8C_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO5553888.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGMGMAN"
     gene            complement(2059..2907)
                     /gene="rhaR"
                     /locus_tag="ACHJ8C_004119"
     CDS             complement(2059..2907)
                     /gene="rhaR"
                     /locus_tag="ACHJ8C_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO5553889.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(2981..3817)
                     /gene="rhaS"
                     /locus_tag="ACHJ8C_004120"
     CDS             complement(2981..3817)
                     /gene="rhaS"
                     /locus_tag="ACHJ8C_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO5553890.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            4105..5574
                     /gene="rhaB"
                     /locus_tag="ACHJ8C_004121"
     CDS             4105..5574
                     /gene="rhaB"
                     /locus_tag="ACHJ8C_004121"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO5553891.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTIYQLRALTEQQPELIPHIAHALLI
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            5571..6830
                     /gene="rhaA"
                     /locus_tag="ACHJ8C_004122"
     CDS             5571..6830
                     /gene="rhaA"
                     /locus_tag="ACHJ8C_004122"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO5553892.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQITPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDRIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRTYEKEILSRRG"
     gene            6923..7747
                     /gene="rhaD"
                     /locus_tag="ACHJ8C_004123"
     CDS             6923..7747
                     /gene="rhaD"
                     /locus_tag="ACHJ8C_004123"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO5553893.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQILVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            7757..8071
                     /gene="rhaM"
                     /locus_tag="ACHJ8C_004124"
     CDS             7757..8071
                     /gene="rhaM"
                     /locus_tag="ACHJ8C_004124"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO5553894.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYVIY
                     LDNARNLLFATIEIESEERWNAVASTDVCQRWWKYMTDVMPANADNSPVSSELQEVFY
                     LP"
     gene            complement(8112..9506)
                     /locus_tag="ACHJ8C_004125"
     CDS             complement(8112..9506)
                     /locus_tag="ACHJ8C_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO5553895.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAEGQVKQLQTQQAAEIREIKTAQGNTPVNGQSTTESEKKNATPPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYNIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKIHGDDS
                     DDRYGARVRFKYFF"
BASE COUNT         2368 a         2485 c         2456 g         2389 t
ORIGIN      
        1 ttttatcgga tcattgtttt tattttgaaa ttgatcacaa aaaaaccacc gccgttggcg
       61 atggttcatt ttttaagctg caatgcggcg taaacgcctc attgcagcag gcggcaaatg
      121 attatttttt cgccgcgaaa cgtgccgctg cttcgtccca gttcaccacg ttccagaact
      181 ctttaatgta gtccggacgg cggttctgga atttcagata gtaagcatgt tcccacacat
      241 ccaggcccag aatcgggaag ccggaagcgc cagaaatggc ttcgcccatc agcggagagt
      301 cctggttagc agtagaaacc accgccagtt tatcgccttt ttgcaccagc catgcccaac
      361 cggaaccaaa gcgggaagct gccgcttttt caaattctgc tttgaaatta tcaacggaac
      421 cgaaatcacg ttcgatagcc gctttcaggt cgccctgcag ggtggtgcct tttttcagac
      481 ctttccagaa cagactgtgg ttagcgtgac caccagcgtt attgcgcagt acggttttct
      541 tgtctgctgg cagctggtcc agtttggtga tcagctcttc aaccggcagg ttggcaaatt
      601 ctggcaggtt ttccagcgcc gcgttggcgt tgttgacgta agtctgatgg tgtttggtgt
      661 ggtggatttc catggtctgc ttatcgaagt gcggttccag ggcatcgtaa gcatacggca
      721 gggatggcag ggtataactc atattcatct ccagtgttgt cgggcggccg attgttaatg
      781 ccgcgtaagc agttggttca ttatagttaa ttaaatgata ttgaaaatga ttatcaatgc
      841 cgtacttttc ataagggtat ggttttgcag gaaaatgccc aagatgtgaa gcaaatcacc
      901 cacttaatgc cgtgattgcc agtaaatcgg cagcggcggc aacaggcgaa aggttaatcg
      961 acagctcaat ttttacactc atctcgtcgg agatgtgacg cgacgaaaaa atgatgagga
     1021 taagaagatg agtaacgcga ttacgatggg gatattttgg catttgatcg gcgcggccag
     1081 tgcagcctgt ttttacgctc cgttcaaaaa agtaaaaaaa tggtcatggg aaaccatgtg
     1141 gtcagtcggt gggattgttt cgtggattat cctgccgtgg gccatcagcg ccctgttact
     1201 accgaatttc tgggcgtatt acagctcgtt tagtctctct acgctgctgc ctgtttttct
     1261 gttcggcgct atgtggggga tcggtaatat caactacggc ctgaccatgc gttatctcgg
     1321 catgtcgatg ggaattggca tcgccattgg cattacgttg attgtcggta cgctgatgac
     1381 gccaattatc aacggcaatt tcgatgtgct gattaatacc gaaggcggac gcatgacgtt
     1441 gctcggcgtt ctggtggcgc tgattggcgt agggattgta actcgcgccg ggcagttgaa
     1501 agagcgcaag atgggcatta aagccgaaga gttcaacctg aaaaaagggc tggtgctggc
     1561 ggtgatgtgc ggcattttct ctgccgggat gtcctttgcg atgaacgccg caaaaccgat
     1621 gcatgaagcc gctgctgcac tgggcgtcga tccactgtat gtcgctctgc caagctatgt
     1681 tgtcatcatg ggcggcggcg cgatcattaa cctcggtttc tgcttcattc gtctggcaaa
     1741 agtgaaggat ttgtcgctaa aagccgactt ctcgctggca aaaccgctga tcattcacaa
     1801 tgtgttactc tcagcactgg gcgggttgat gtggtatctg caattctttt tctatgcctg
     1861 gggccacgcg cgtattccgg cgcagtatga ctacatcagt tggatgctgc atatgagctt
     1921 ctatgtattg tgcggcggta tcgtcgggtt ggtgctgaaa gagtggaaca atgcaggtcg
     1981 ccgtccggta acggtgttga gcctcggttg tgtggtgatt attgtcgccg ccaacatcgt
     2041 cggcatgggc atggcgaatt aatctttctg cgaattgaga tgacgccact ggctgggcgt
     2101 catcccggtt tcccgggtaa acaccaccga aaaatagtta ctatcttcaa agccacattc
     2161 ggttgaaata tcactgatta acaggcggct atgctggaga agatattgcg catgacacac
     2221 tctgacctgc cgcagatatt gattgatggt cattccagtc tgctgacgaa attgctgacg
     2281 caaaacgcgc tcactgcacg atgcctcatc acaaaattta tccagcgcaa agggactttt
     2341 caggctagcc gccagccggg taatcagctt atccagcaac gtttcgctgg atgttggcgg
     2401 caacgaatca ctggtgtaac gatggcgatt cagcaacatc accaactgcc cgaacagcaa
     2461 ctcagccatt tcgttagcaa acgacacatg ctgactactt tcatgctcaa gctgaccgat
     2521 aacctgccgc gcctgcgcca tccccacgct acctaagcgc cagtgtggtt gccctgcgct
     2581 ggcgctaaat cccggaatcg ctccctgcca gtcaagattc agcttcagcc gctccgggca
     2641 ataaataata ttctgcaaaa ccagatcgtt aacggaagcg taggagtgtt tatcgtcagc
     2701 atgaatataa aagagatcgc cacgggtaat gcgataaggg cgatcgttga gtacatgcag
     2761 accattaccg cgccagacaa tcaccagctc acaaaaatca tgtgtatgtt cggcaaagac
     2821 atcttgcgga taacggtcag ccacagcgac tgcctgctgg tcgctggcaa aaaaatcatc
     2881 tttgagaagt tttaactgat gcgccaccgt ggctacctcg gccagagaac gaagttgatt
     2941 attcgcaata tggcgtacaa atacgttgag aagattcgcg ttattgcaga aaaccatccc
     3001 gtccctgacg aatatcacgc ggtgaccagt taaactctcg gcgaaaaagc gtcgaaaagt
     3061 ggttactgtc gctgaatccg cagcgatagg cgatgtcagt aacgctggcc tcgctgtggc
     3121 gtagcagatg tcgggctttc atcagtcgca ggcggttcag gtaacgctga ggcgtcagcc
     3181 ccgtttgctg cttaagctgg cgatgcagcg tacgcaatga aagagaaaat tgatccgcca
     3241 cagcgtccca attcacctca tcggcaaaat ggtcctccag ccaggccaga agcaagttga
     3301 gacgtgatgc gctgttttcc aggttctcct gcaaactgct tttacgcagc aagagcagta
     3361 attgcataaa cagaatttca cgactggcgg tcgagggtaa atcattttcc ccttcctgct
     3421 gttccatctg tgcaaccagc tgtcgcacct gctgcaatac gctgtggtta acacgccagt
     3481 gagacggata ctgcccatcc tgctcttgtg gcagcaactg attcagcccg gcgagaaact
     3541 gaaatcgatc cggcgagcga tacagcacat tggtcagaca cagattatcg gtatgttcat
     3601 acagatgccg atcatgatcg cgtacgaaac agaccgtccc accggtgatg gtatagggct
     3661 gcccattaaa tacatgaata cccgtgccat gttcgacaat cacaatttca tgaaaatcat
     3721 gatgatgttc aggaaaatcc gcctgcggga gccggggttc tatcgccacg gacgcgttac
     3781 cagacggaaa aaaatccaca ctatgtaata cggtcatact ggcctcctga tgtcgtcaac
     3841 acggcgaaat agtaatcacg aggtcaggtt cttaccttaa attttcgacg gaaaaccacg
     3901 taaaaaacgt cgatttttca agatacagcg tgaattttca ggaaatgcgg tgagcatcac
     3961 atcaccacaa tgaagcaaat tgtgaacatc atcacgttca tctttccctg gttgccaatg
     4021 gcccattttc ctgtcagtaa cgagaaggtc acgaattcag gcgcttttta gactggtcgt
     4081 aatgaaattc agcaggatca cattatgacc tttcgcaatt gtgtcgccgt cgatctcggc
     4141 gcatccagtg ggcgcgtgat gctggcgcgt tacgagcgtg aatgccgcag cctgacgctg
     4201 cgcgaaatcc atcgttttaa caatggcctg catagccaga acggttatgt cacctggaat
     4261 gtggatagcc tggaaagtgc cattcgcctt ggattaaaca aggtgtgcga ggaagggatt
     4321 cgtatcgata gcattgggat tgatacctgg ggcgtggatt ttgtgctgct cgaccaacag
     4381 ggtcagcgtg tgggcctgcc cgttgcttat cgcgatagcc gcaccaatgg cctaatggcg
     4441 caggcacaac aacaactcgg caaacgcgat atttatcaac gtagcggcat ccagtttctg
     4501 cccttcaata cgatctatca gctacgtgca ctgacagagc agcaaccaga acttattcca
     4561 cacattgctc acgctttgct gataccggat tactttagtt atcgcctgac cggcaagatg
     4621 aactgggaat ataccaacgc cacgaccacg caactggtca atatcaatag cgacgactgg
     4681 gacgagtcgc tactggcgtg gagcggtgcc aacaaagcct ggtttggtcg cccgacgcat
     4741 ccgggtaatg tcataggtca ctggatttgc ccgcagggta atgagattcc ggtggtcgcc
     4801 gttgccagcc atgataccgc cagcgcggtt atcgcctcgc cgttaaacgg ttcacgcgcc
     4861 gcttatctct cttctggcac ctggtcattg atgggcttcg aaagccagac gccatttacc
     4921 aatgacacgg cgctggcagc caatatcacc aacgaaggcg gggcggaagg tcgctatcgg
     4981 gtgctgaaaa atattatggg cttatggctg cttcagcgag tgcttcagga gcggcaaatc
     5041 aacgatcttc cggcgcttat cgccgcaaca caggcacttc cggcctgccg cttcatcatc
     5101 aatcccaatg acgatcgctt tattaatcct gacgagatgt gcagcgaaat tcaggctgcg
     5161 tgtcgggaaa cggcgcaacc gatcccggaa agtgatgctg aactggcgcg ctgtattttc
     5221 gacagtctgg cgctgctgta tgccgatgtg ttgcatgagc tggcgcagct gcgtggtgaa
     5281 gatttctcgc aactgcatat tgtcggcggc ggctgccaga acacgctgct caaccagctg
     5341 tgtgccgatg cctgcggtat tcgggtgatc gcagggcctg ttgaagcctc gacgctcggc
     5401 aatatcggca tccagttaat gacgctggat gaactcaaca atgtggatga tttccgtcag
     5461 gtcgtcagca ctaccgcgaa tctgaccacc tttaccccta atcctgacag tgaaattgcc
     5521 cactatgtgg cgcagattca ctctacacga cagacaaagg agctttgcgc atgaccactc
     5581 aactggaaca ggcctgggag cttgcgaaac agcgtttcgc ggcagtaggg attgatgtcg
     5641 aggaggcact gcgccaactt gatcgtttac ccgtttcaat gcactgctgg cagggcgatg
     5701 atgtttccgg ttttgaaaac ccggaaggtt cgctgaccgg tggtattcag gctactggta
     5761 attatccggg caaagcgcgt aatgccagcg agctgcgtgc cgatctggaa caggctatgc
     5821 ggctgattcc ggggccgaaa cggcttaatt tacatgccat ctatctggaa tcagacacgc
     5881 cagtctcgcg cgatcagatc acaccagagc acttcaaaaa ctgggttgaa tgggcgaaag
     5941 ccaatcagct cggtctggat tttaacccct cctgtttttc gcatccgcta agcgccgatg
     6001 gctttacgct ttcccatgcc gacgaccgca ttcgccagtt ctggattgac cactgtaaag
     6061 ccagccgccg tgtttcagcc tattttggtg agcaactcgg cacaccgtcg gtgatgaaca
     6121 tctggatccc ggatggcatg aaagatatta ccgttgaccg tctcgcccca cgccagcgtt
     6181 tgctggcagc actggatgaa gtgatcagcg agaagctaaa ccctgcgcac catatcgacg
     6241 ccgttgagag caaattgttt ggcattggcg cggagagcta cacggttggc tccaatgagt
     6301 tttacctggg gtatgccacc agccgccaga cagcgctgtg cctggacgcc gggcatttcc
     6361 acccgactga agtgatttcc gacaagattt ccgccgccat gctgtatgtg ccgcagttgc
     6421 tgctgcacgt cagccgtccg gttcgctggg acagcgatca cgtagtgctg ctggatgatg
     6481 aaacccaggc gattgccagt gagattgttc gtcacgatct gttcgaccgg gtgcatatcg
     6541 gcctcgactt cttcgatgcc tctatcaacc gcattgccgc gtgggtcatt ggtacacgca
     6601 atatgaaaaa agccctgctg cgtgcgttgc tggaacctac cgctgaacta cgcaagctgg
     6661 aagcggcggg cgattacact gcgcgtctgg cgctactgga agagcagaaa tcgttgccgt
     6721 ggcaggcggt atgggaaatg tattgtcagc gtcacgatac gccagcgggt agcgaatggc
     6781 tggagaacgt gcggacttat gagaaagaaa ttttgagtcg ccgcgggtaa acactgccgg
     6841 atgcggcaca agtgccttat ccggcctaca gatcgtacat ccggcaacag gcacggacgt
     6901 ccaattaaca ggaacacaga acatgcaaaa cataacccag tcctggtttg tccagggaat
     6961 gatcaaagcc accaccgatg cctggctgaa aggctgggac gagcgcaacg gcggcaacct
     7021 gacgctgcgc ctggatgacg ccgatatcgc accatatcac gacaattttc accaacaacc
     7081 gcgctatatc ccgctcagcc agcccatgcc tttactggca aatacaccgt ttattgtcac
     7141 cggctccggc aaattcttcc gtaacgtcca gcttgatcct gcggctaact taggcatcgt
     7201 aaaagtcgac agcgacggcg cgggctacca cattctttgg gggttaacca acgaagccgt
     7261 ccccacttcc gaacttccag ctcacttcct ttcccactgc gagcgcatta aagccaccaa
     7321 cggcaaagat cgggtgatca tgcactgcca cgccactaac ctgatcgctc tcacctacgt
     7381 gctggaaaac gacaccgcgg tcttcactcg ccaactgtgg gaaggcagca ccgagtgtct
     7441 ggtggtattc ccggatggcg ttggcattct gccgtggatg gtacccggca cggacgaaat
     7501 cggccaggca actgcacaag agatgcaaaa acattcgctg gtgttgtggc ccttccacgg
     7561 tgtgttcggt tccggaccga cactggatga aaccttcggt ttaatcgaca ccgcagaaaa
     7621 atcagcacaa atattagtga aggtttattc gatgggcggc atgaaacaga ccatcagccg
     7681 tgaagagttg atagcgctcg gccagcgttt cggcgttaca ccactcgcca gtgcgctggc
     7741 gctgtaagga gcaaacatga tccgcaaagc ctttgtcatg caggtaaacc ccgacgccca
     7801 cgaagagtat cagcgtcggc ataatcccat ctggccggaa ctggaagcag tgctgaaatc
     7861 tcacggtgcg cataactacg tcatctatct cgacaatgcg cgtaatctgc tatttgccac
     7921 gatagagatt gaatctgaag agcggtggaa tgcggttgcc agtacagatg tttgccaacg
     7981 ttggtggaaa tatatgaccg acgttatgcc cgctaatgcg gataacagcc cagtcagtag
     8041 cgaactgcaa gaagtgtttt acctgcctta atgagttgcg gtgccggaaa atatccagca
     8101 ccgccctttc atcagaagaa gtatttaaag cgaacgcgtg cgccgtaacg gtcatcacta
     8161 tcgtcgccgt gtatcttatc gccgtcgcta tccagaatgg agtaatacgt gccgaggtaa
     8221 atattaaagt tctccatatc catcacatta gcgaactgat aagacgcatg aatggtgtga
     8281 atgttgtatg ttccttcatc atcaatccag caatcgttat cacaaaccac gccactaaat
     8341 tcatcaatct tattatgtgc atagatataa cccagctcga aacgtttcca cagcgcgtta
     8401 atccctgccg tgaaatcttt ttcattatta gcatctaaat aggcggtatt aagattaacc
     8461 acgatgccat tttccggatc ggttttcagg ccattccagg tcatgctcat gccataaccg
     8521 gtacgatcgg actgatcgac aaaattaccc ttgctatcgg tataaccata agcattattt
     8581 accacattcg cttccatcgc tgcggaaacg gtgaattctt ccgtcggcga ccaggcaatt
     8641 accggacgca gataagcaac atttttctgc tgttccatat cgcgtccatg ataattaccg
     8701 tcgttatata aagatgttcc gtcttccagt aacgtgttta attcaaaata ccagttatcg
     8761 agttgtttgc tgacgaggaa attaccgcca gcgttagaac gtccgcggcc ctctttcatc
     8821 atatagatat aaccgctgcc atcgtcataa agatcgttcg cagtattacc ggaatgttca
     8881 acaaaggtat cctgattcag cgggaacata tcgtaggctt caaaacggcc tactttgacc
     8941 ttccagtcgt tttctttgcc aaagaagaaa accgcatcat cgatatttac tgaaccgtgc
     9001 atatccccca gcggttgtgc ggagaaccca gcgaaatagc cattatccag ttttcgcata
     9061 ccatcaaaac ctaacagaat acgaccattg agattccact gttcattagt gggatcgcta
     9121 ttcacatcag cgttggtcat tgccagcagg ccatgattac tttccgcatc catattaaat
     9181 tctacgtcac cgtagatttt taaatcgcca taccctgaaa gcaggagatt aggcggggtg
     9241 gcgtttttct tctctgactc cgtcgttgat tgaccgttta ccggcgtgtt gccctgtgcg
     9301 gttttaattt cgcggatctc ggcggcttgt tgtgtctgaa gctgtttaac ctgcccctct
     9361 gcttttgctg cccgcatttc tgccgcttcc aggcgttctt ccagcaatgc catgcgttgt
     9421 tccagcgtta aatgctgtgt ttttgccata ccggtactac atgccaataa taaaccgatg
     9481 gctaaagata atgtcgattt tttcattatt tacgttccct gaaataaagg agaccctacg
     9541 ctttccaaaa ggaatacgta tatagagaaa tataaggtgt gcactttcga atttttaatc
     9601 caacttcagc aggccgatgg atagtatccc gccgcataat atgagtaaat ccgctgattt
     9661 cctggaacgt gaggaatata acaatagtgc cgttttta
//
LOCUS       DAZFJL010000165         9658 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFJL010000165 DAZFJL010000000
VERSION     DAZFJL010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.167"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            113..1090
                     /gene="glaH"
                     /locus_tag="ACHJ8C_004126"
     CDS             113..1090
                     /gene="glaH"
                     /locus_tag="ACHJ8C_004126"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO5553896.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLPELTFTEQTTNR
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDLFFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLHRELMRQRGYFAYATHHYQTHQ"
     gene            1110..2378
                     /gene="lhgO"
                     /locus_tag="ACHJ8C_004127"
     CDS             1110..2378
                     /gene="lhgO"
                     /locus_tag="ACHJ8C_004127"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO5553897.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCEQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIDREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVICTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEIKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPPGVRAQAVSPDGKLIDDFLFVTTTRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFYQ"
     gene            2401..3849
                     /gene="gabD"
                     /locus_tag="ACHJ8C_004128"
     CDS             2401..3849
                     /gene="gabD"
                     /locus_tag="ACHJ8C_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO5553898.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPTGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQNGVYDRFAEKLQQAVSKLHIGNGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIIGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            3863..5143
                     /gene="gabT"
                     /locus_tag="ACHJ8C_004129"
     CDS             3863..5143
                     /gene="gabT"
                     /locus_tag="ACHJ8C_004129"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO5553899.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHTEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKL"
     gene            5380..6780
                     /gene="gabP"
                     /locus_tag="ACHJ8C_004130"
     CDS             5380..6780
                     /gene="gabP"
                     /locus_tag="ACHJ8C_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO5553900.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVLSTGLLAIGIICTVPIMAR
                     WKKLIMWQKTPIHNTR"
     gene            6801..7463
                     /gene="csiR"
                     /locus_tag="ACHJ8C_004131"
     CDS             6801..7463
                     /gene="csiR"
                     /locus_tag="ACHJ8C_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO5553901.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMCQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7464..7913)
                     /gene="kbp"
                     /locus_tag="ACHJ8C_004132"
     CDS             complement(7464..7913)
                     /gene="kbp"
                     /locus_tag="ACHJ8C_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO5553902.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(7997..8155)
                     /gene="yqaE"
                     /locus_tag="ACHJ8C_004133"
     CDS             complement(7997..8155)
                     /gene="yqaE"
                     /locus_tag="ACHJ8C_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO5553903.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8338..8637
                     /gene="ygaV"
                     /locus_tag="ACHJ8C_004134"
     CDS             8338..8637
                     /gene="ygaV"
                     /locus_tag="ACHJ8C_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO5553904.1"
                     /translation="MTELAQLQASAEQAAALLKSMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRGEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8647..9171
                     /gene="ygaP"
                     /locus_tag="ACHJ8C_004135"
     CDS             8647..9171
                     /gene="ygaP"
                     /locus_tag="ACHJ8C_004135"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO5553905.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSVFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9218..9622)
                     /gene="stpA"
                     /locus_tag="ACHJ8C_004136"
     CDS             complement(9218..9622)
                     /gene="stpA"
                     /locus_tag="ACHJ8C_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO5553906.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFIDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2217 a         2492 c         2649 g         2300 t
ORIGIN      
        1 aaacaatatg taacttttat gcgcattttt cagaaatgta gatattttta gattatggct
       61 acgaaatgag catcgccatg tcaccctaca tctcataaga ggatcgcttc tgatgaatgc
      121 actgaccgcc gtacaaaata acgctgtcga ttcaggccag gactatagcg gattcactct
      181 catcccgtcg gcgcaatccc cgcgtctgcc ggaactcacc ttcaccgaac agacgaccaa
      241 tcggttcctc gaacaggttg ccgagtggcc cgtgcaggcg ctggagtaca aatcgtttct
      301 gcgttttcgg gtaggcaaaa ttctcgacga tctgtgtgcg aatcagctgc aaccgctgct
      361 gttgaagacc ctgttaaacc gcgctgaagg tgcgctgttg atcaatgcgg tgggtatcga
      421 tgatgtcgcg caggcggatg agatggtgaa gctggcgacg gcggtggcgc atctgattgg
      481 tcgctccaat tttgacgcca tgagcggtca gtattacgcg cgtttcgtgg tgaaaaatgt
      541 tgataactca gacagctatc ttcgtcagcc gcaccgcgta atggagctgc acaacgacgg
      601 cacttacgtc gaagagatca ctgattacgt gctgatgatg aaaatcgacg agcagaacat
      661 gcagggcggt aactcgctgc tgctgcatct cgatgactgg gaacatctgg acctcttttt
      721 ccgccatccg ctggcgcgcc gtccgatgcg ctttgccgcg ccgccgagca aaaacgtcag
      781 caaagatgtc ttccatccgg tgttcgatgt cgatcagcag ggccgcccgg tgatgcgcta
      841 tatcgaccag tttgtccagc cgaaagactt cgaagaaggc gtgtggttga gcgagctttc
      901 cgatgccatt gaaaccagta aaggcattct ttctgtaccc gttcccgttg gcaaattcct
      961 gttgattaac aacctgttct ggctgcacgg tcgcgaccgc tttactccgc acccggatct
     1021 gcaccgtgaa ctgatgcgtc agcgtggcta tttcgcttac gccactcacc actaccagac
     1081 gcatcagtaa gcgcaaagga attgagcgga tgtatgattt tgtgattatt ggcggcggca
     1141 tcatcggcat gtcgaccgcc atgcaactga ttgatgttta tccggatgcc cgcattgcgt
     1201 tgctggaaaa agagtccggc ccggcctgtc accagacggg ccacaacagc ggcgtaatcc
     1261 atgccggggt ctattacacg cccggcagcc tgaaggcgca gttttgcctg gcaggaaacc
     1321 gcgccaccaa agccttttgc gagcaaaacg gcattcgcta cgacaactgc ggcaagatgc
     1381 tggtcgccac gtctgaactc gaaatggaac ggatgcgcgc cttatgggaa cgcacggcgg
     1441 cgaacggaat cgatcgcgag tggttaaacg ccgaagagct gcgcgagcgc gaaccaaata
     1501 tcaccgggct gggcggtatt tttgtgccgt ccagcggcat tgtcagctac cgcgaagtga
     1561 cggcggcaat ggcaaaaatt tttcaggcca gaggcggtga gattatctat aacgccgaag
     1621 tcagcgccct cagtgagcat aaaaacggcg tggtgatatg tacccgtcag ggcggtgaat
     1681 atgaagcatc gacgctgatt agctgttccg ggctgatggc tgaccggctg gtgaaaatgc
     1741 tcggcctcga accgggcttt attatctgcc cgttccgcgg cgagtatttc cgccttgcgc
     1801 cggagcataa ccagattgtt aaccacctga tttaccccat tcccgacccg gcaatgccgt
     1861 ttttgggcgt gcatctcacc cgcatgatcg acggcagcgt gaccgtcggg ccaaacgcgg
     1921 tgctggcttt caaacgcgaa ggctatcgta agcgcgactt ctcatttagc gacacgctgg
     1981 aaattttggg ctcgtcgggg attcgccggg tgctgcaaaa ccatctacgc tcaggactgg
     2041 gcgagataaa aaactcgctg tgcaaaagcg gctatctgcg gctggtgcaa aagtattgtc
     2101 cccggctttc gttaagcgat ctccagccct ggccccccgg tgtgcgggcg caggcagtat
     2161 cgccggacgg caagctgatt gacgattttc tgtttgtcac caccacgcgc acgatccaca
     2221 cctgtaatgc gccctccccg gcagcgacat cagcaattcc tattggtgcg catattgtca
     2281 gcaaggtaca aacgctgttg gcaagccaga gtaaccccgg acgcacgctg cgagcggcac
     2341 gtagtgtgga tgccttacac gccgcatttt atcaataacc tttgaaaaca ggatgtagcg
     2401 atgaaactta acgacagtaa cttattccgc cagcaggcgt tgattaacgg ggaatggctg
     2461 gacgccaaca atggcgaggt catcgacgtc accaatccgg cgaacggcga caagctgggt
     2521 agcgtgccga aaatgggcgc tgatgaaacc cgcgccgcta tcgacgccgc caaccgcgcc
     2581 ctgcccgcct ggcgtgcgct cactgccaaa gaacgcgcca acattctgcg caactggttc
     2641 aatttaatga tggagcatca ggacgattta gcgcgcctga tgaccctcga acagggcaaa
     2701 ccactggctg aagcgaaagg cgaaatcagc tacgccgcct catttattga gtggtttgca
     2761 gaagaaggca aacgtattta tggcgacacc attcccggtc atcaggccga taaacgcctg
     2821 attgttatca agcagccgat tggcgtcacc gcggctatca cgccgtggaa cttcccggcg
     2881 gcgatgatta cccgtaaagc cggtccggcg ctggcggcag gctgcacgat ggtgctgaag
     2941 cccgccagtc agacgccgtt ctctgcgctg gcgctggcag aactggcgat tcgcgcgggc
     3001 gttccgactg gcgtgtttaa cgtggtcacc ggttcggcgg gcgcggtcgg taacgaactg
     3061 accagcaacc cgctggtgcg caagctgtcg tttaccggct cgaccgaaat tggtcgccag
     3121 ttaatggaac agtgcgcgaa agacatcaaa aaagtgtcgc tggagctggg cggcaacgca
     3181 ccgtttattg tctttgacga tgccgatctc gataaagccg tggaaggcgc gctggcctcg
     3241 aaattccgta acgccgggca aacctgcgtc tgcgccaacc gcctgtatgt gcagaacggc
     3301 gtgtatgacc gctttgccga aaaattgcag caggcggtga gcaaactgca catcggcaac
     3361 gggctggaga aaggcgtcac catcgggccg ctgatcgatg aaaaagcggt agcaaaagtg
     3421 gaagagcata ttgccgatgc gctggagaaa ggcgcgcgtg tggtttgcgg cggtaaagca
     3481 catgaacgcg gcggtaactt cttccagccg accattctgg tggacgttcc ggctaacgcc
     3541 aaagtgtcga aagaagagac gtttggcccc ctcgccccgc tgttccgttt taaagatgaa
     3601 gccgatgtga ttgcgcaagc caatgacacc gaatttggac ttgcagccta tttctacgcc
     3661 cgtgatttaa gccgcgtctt ccgcgtgggc gaagcactgg agtacggcat catcggcatc
     3721 aacaccggca ttatttccaa tgaagtggcc ccgtttggcg gcattaaagc ctcgggtctg
     3781 ggtcgtgaag gttcgaagta tggcatcgaa gattacttag aaatcaaata tatgtgcatc
     3841 ggtctttaac tggagaatgc gaatgagcag caataaagag ttaatgcagc gccgcagtca
     3901 ggcgattccc cgtggcgttg ggcaaattca cccgattttc gctgaccgcg cggaaaactg
     3961 ccgggtgtgg gacgttgaag gccgtgagta tcttgatttc gcgggcggga ttgcggtgct
     4021 caataccggg cacctgcatc cgaaagtggt tgcggcggtg gaagcgcagt tgaaaaaact
     4081 gtcgcacacc tgcttccagg tgctggctta cgagccgtat cttgaactgt gcgagattat
     4141 gaatcagaag gtgccgggcg attttgccaa gaaaactctg ctggttacga ccggttccga
     4201 agcggtggaa aatgcggtga aaatcgcccg cgccgccacc aaacgtagcg ggaccatcgc
     4261 ctttagcggt gcgtatcacg ggcgcacgca ttacacgctg gcgctgaccg gcaaggtgaa
     4321 tccgtactct gcgggcatgg ggctgatgcc tgggcacgtt tatcgcgcgc tttatccttg
     4381 cccgctgcac ggcatcagtg aagatgacgc tatcgccagc attcaccgaa tttttaaaaa
     4441 tgatgctgcg ccggaagata tcgccgccat cgtgattgag ccggttcagg gcgaaggcgg
     4501 tttctacgcc gcgacgccag cctttatgca gcgtttacgc gcgctgtgtg acgagcacgg
     4561 gatcatgctg attgccgatg aagtgcagag cggcgcaggg cgtaccggca cgctgtttgc
     4621 gatggagcaa atgggcgttg cgccggatct taccaccttt gcgaaatcga tcgcgggcgg
     4681 cttcccgctg gcgggcgtca ccgggcgcgc ggaagtgatg gatgccgtcg ctccaggcgg
     4741 tctgggcggc acctatgccg gtaacccgat tgcctgcgtg gcagcgctgg aagtgttaaa
     4801 ggtattcgag caggaaaatc tgctgcaaaa agccaacgat ctggggcaga aactgaaaga
     4861 cggattgctg gcgatagccg aaaaacatac tgagatcggc gacgtacgcg ggctgggagc
     4921 gatgattgcc atcgagctgt ttgaagatgg cgatcacagc aaaccagacg caaaactcac
     4981 cgccgagatc gtggcgcgtg cccgcgataa gggtctgatt cttctctcct gcggcccgta
     5041 ttacaacgtg ctgcgcatcc ttgtaccgct caccattgaa gacgctcaga tccgtcaggg
     5101 tctggaaatc atcagccagt gttttgatga ggcgaagctg tagcaccgca ccatgccgga
     5161 ggcgacgctt tgcggcttgt ccggcctacg agaatccagg tcggataagg cgtttacgcc
     5221 gcatccgaca atctgtacgt gaacaggaat aaatctatgt cggccggata aggcggagcc
     5281 gctctccggc aaaaagaacc aataacaatt aaacgcgtga cccgacgcgg gaaatgtcgg
     5341 ggcgctctcc caagtgacac actttcgaga ggattcagga tggggcaatc atcgcaacca
     5401 catgagttag gcggcgggct gaagtcacgc cacgtcacca tgttgtctat tgccggtgtt
     5461 atcggcgcaa gtctgtttgt cggttccagc gtcgccatcg ccgaagcggg cccggcggta
     5521 ttactggcct atctgttcgc cggattactg gtggttatga ttatgcggat gttggcggaa
     5581 atggcggttg ccacgcccga taccggttcg ttttccacct atgccgataa agccattggc
     5641 cgctgggcgg gctataccat cggctggttg tactggtggt tttgggtact ggttatccca
     5701 cttgaagcca acatcgccgc catgatcctg cactcgtggg ttccaggcat tcccatctgg
     5761 ttattttccc tcgtcattac cctcgcctta accggcagta atttattaag cgttaaaaac
     5821 tacggcgaat ttgagttctg gctggcgctg tgtaaagtca tcgctatcct ggcctttatt
     5881 ttccttggtg cagtcgcaat tagcggtttt tacccttatg ccgacgtgag cggtatttca
     5941 agattgtggg atagcggcgg ctttatgccc aacggcttcg gtgcggtatt aagcgcgatg
     6001 ttgatcacca tgttctcgtt tatgggcgca gaaattgtca ccattgccgc cgcggaatcc
     6061 gacacgccgg aaaaacatat tgtccgcgcc accaactcgg ttatctggcg tatctctatt
     6121 ttctatttgt gttctatttt tgtcgttgtg gcattaatcc cgtggaatat gccggggctg
     6181 aaagccgttg gttcttatcg ctcggttctg gaattgctca atattcctca tgcgaaatta
     6241 atcatggact gcgtgatatt actttccgta accagttgcc tgaactcggc gctgtatacc
     6301 gcttccagga tgctctactc cttaagccgt cgcggtgatg ctcccgcggt aatgggtaaa
     6361 atcaaccgca gtaaaacccc gtacgtggcg gtgttactct ccaccggcgc ggcattctta
     6421 acggtggtgg tgaactatta cgcgcctgcg aaggtgttta aatttctgat cgacagctcc
     6481 ggcgctatcg ccctgctggt ttatttagtc atcgccgttt cacaattgcg gatgcgcaaa
     6541 attctgcgag cagaaggaag cgaaattcgc ctgcggatgt ggctttatcc gtggctcacc
     6601 tggctggtaa tcggttttat tacctttgtg ttggtagtga tgctattccg cccggcgcaa
     6661 cagttagaag tgctctccac cggcttatta gcgattggga ttatctgtac cgtgccaatt
     6721 atggctcgct ggaaaaagct gataatgtgg caaaaaacac ccattcataa tacgcgctga
     6781 taatgatcag gagtcacacc atgaccatta cctctctgga tggctatcgc tggctgaaga
     6841 acgatattat tcgcggcaat tttcagccgg atgaaaaatt acggatgagt ttgctgacat
     6901 cgcgttatgc acttggcgtc ggaccgttgc gggaagcgct ttcgcaactg gtggcagaac
     6961 gactggtcac ggtggtgaat caaaaagggt atcgggtggc gtctatgtca gagcaggagc
     7021 tgctcgatat tttcgacgcc cgcgccaata tggaagcgat gctggtgagt ctggcgatag
     7081 cccgcggtgg cgatgaatgg gaggcagacg ttctcgcaaa agcgcatctg ctcagtaagc
     7141 ttgaggcctg tgacgccagt gagaaaatgc ttgatgagtg ggatctgcgt catcaggcgt
     7201 ttcatactgc aattgtggcg ggctgtggtt ctcactattt gctgcaaatg cgtgaacggt
     7261 tgtttgatct ggcggcgcgc tatcgattta tctggctgcg gcgaacagtg ctttcggtgg
     7321 aaatgctgga ggataaacac gatcagcacc agaccctgac tgcggcggta ctggcgcgag
     7381 ataccgcgcg cgccagtgag ttaatgtgcc agcatttact gacgccaatt cccattatcc
     7441 agcaggcgat ggctggcaat taactactct tcaggaatac gcaacacttg ccccgggtaa
     7501 attttatccg ggctttttag catcggttta ttcgcttcga agattttatt gtacagatta
     7561 gcgttaccgt agacctgttt ggaaatggca ctcagagtgt cgccagactt aacggtataa
     7621 aactggctgg cagtggctgg tgtcgccgtt ttcacctgat catcgacact ggcaataccg
     7681 gaaatattcc ccaccgcaac aaggattttc tcctttgcct cctgacttaa gccgtcacca
     7741 gtgaccgtcg ctttgccgtc ggcaatttga atgttcactt tatcggcatc aggtataccg
     7801 gttttgttca gatgctcctg caccttcttc gcctgatcgt ctttatcgtg ctgacctgta
     7861 accgcgtccc agagtttttc tccggcatct ttcacaaaat tgaacaaacc catagctacc
     7921 tcattcgtta acggaaacac tcagaaagtg tagcagagga acgcggcgcg ttaacgccgc
     7981 gggggtgagg acaggtttaa tcgcgggttt gcacccagaa cgcgtgaatc aaaccgggga
     8041 tatagccaag cagtgtcagc agaatattaa taatgaacgc ccaaccgaac cctttaccga
     8101 gcagcacgcc gagcggcggc agaatgatgg tgatgacgat tctccagaaa cccatatgta
     8161 ctccctataa gaaaattact cattgtttaa aaagagattt tatctcttaa gcgtagtaat
     8221 tttagcggag cctgccagtt tcaacttacg cttttactct catttacgct gctgatgctg
     8281 gtatatgttg cacaattatt ttagagttta cttaatttag ataatactta aataactatg
     8341 actgaactcg cacaattaca ggccagtgcc gaacaggcag cggccttatt gaaatcaatg
     8401 agccacccta aacgattgct gattctgtgt atgcttagcg gttcccccgg caccagcgcg
     8461 ggagagctga cgcgcattac cggactgagt gcctctgcga catcacagca tctcgctcgt
     8521 atgcggggcg aagggcttat cgacagccaa cgggatgccc aacgcattct atattccatt
     8581 aaaaatgagg cggtaaatgc cattatcgcc accctgaaaa atgtctattg tccgtaagga
     8641 gtcactatgg ctttgacaac catttcgccg catgatgcac aagaattaat cgcacgcggc
     8701 gcaaagttaa tcgatattcg tgatgctgat gaatatcttc gtgaacatat tcctgaagca
     8761 gatctagctc cattatccgt actggaacag tcaggtcttc cgcctaaatt acgtcgcgag
     8821 caaattatat tccactgcca ggcaggtaaa cgcaccagta ataacgccga taaattagca
     8881 gcgattgccg cccccgcaga aatcttttta ctcgaagatg ggattgatgg ctggaaaaaa
     8941 gcaggattgc cagtagcggt aaataaatcc caacccttgc cgttaatgcg ccaggtgcag
     9001 atcgccgcgg gtggtttaat attaatcggc gttgtactgg gttataccgt aaatagcgtt
     9061 ttcttcttat taagcggctt tgttggtgct gggttactgt ttgcaggaat tagtggtttt
     9121 tgtggaatgg caaggttgtt agataagatg ccgtggaacc aacgagcttg agaagcgacg
     9181 ccggacgcgc catagcagcg acatccggcc tcagtaatta gatcaggaaa tcgtcgagag
     9241 atttaccttc tgccagcgcc tgagcgattg gcttcggtgt acggccctga ccggtccagg
     9301 ttttagtttc accgttaaca tcgatgaatt tatatttcgc cggacgcggc tggcgttttt
     9361 taccagcgcg tggtgccgca gcagagctat tacccaataa ctcttccggg ttaattccgt
     9421 cagctttcat cagctccagc caggtgctaa ttttttcctg gcgctctgcc agttcacgct
     9481 gctgttgttc ttcttcttca cgtctttctt tagtgacaac cctgaatttt tcgagcattt
     9541 cttcaagaac gtcaatggag aattcgcgag ccatcgcacg gagggtgcga atgttattta
     9601 aactttgtaa cattacggac ataataaaga aaacctttta acccaaaaca aaaaagta
//
LOCUS       DAZFJL010000166         9542 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFJL010000166 DAZFJL010000000
VERSION     DAZFJL010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.168"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..277)
                     /locus_tag="ACHJ8C_004137"
     CDS             complement(<1..277)
                     /locus_tag="ACHJ8C_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553907.1"
                     /translation="MTQAKANLRKLRRARYVPPRTVKKNIANTERVVSKAITGCGVFR
                     DIPQVKINLIETRKILQIELALSIANLSEANPIEIRKKIKSIDRQLSS"
     gene            complement(293..544)
                     /locus_tag="ACHJ8C_004138"
     CDS             complement(293..544)
                     /locus_tag="ACHJ8C_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553908.1"
                     /translation="MKKAFIYIGHTKMSAFIKLASVLALFISPVVVAIAGEQSHCTKI
                     NEYPFIVTQCDDGTVTVVNVINNRVAVCRKGESCKEIKL"
     gene            complement(692..1354)
                     /locus_tag="ACHJ8C_004139"
     CDS             complement(692..1354)
                     /locus_tag="ACHJ8C_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553909.1"
                     /translation="MRNANEGISLAAIVAKETTTGKWNGTGGGIGLGTGGIGLFIGGV
                     SGSKQEQTQRAKNFESPKEEEYSLLNVYGSIIKVLVFGVLFGLSARLFTSLSNTGSDF
                     AEPVTTTTATGKIQSIMGGMAEMVSILGTVVPFLVIITAMIWFLFFSSKREKEELTRV
                     EREAAKTKLRIDVYHRLRYVESDHVVFDPITGREVPAERTCINKLIEALVDESNDISK
                     RN"
     gene            complement(1365..1535)
                     /locus_tag="ACHJ8C_004140"
     CDS             complement(1365..1535)
                     /locus_tag="ACHJ8C_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553910.1"
                     /translation="MHLSLLIPLTGAAMNALCIFIIRLLDKDFIEVYKKTLKKITALH
                     ILLMVLSVTGIV"
     gene            complement(1539..1982)
                     /locus_tag="ACHJ8C_004141"
     CDS             complement(1539..1982)
                     /locus_tag="ACHJ8C_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO5553911.1"
                     /translation="MFWFTLFLLCIALEIITGTGWLLVISLGALSSAIVDFFLPISQE
                     ANICFFASISILTSIIKFVYDKKHKKLDTPLVNTGHTRFKGKEFTLSDDIVNGKGQLL
                     IGDTFWPVETLHDENYPAGTRVIVTDMQGITLKVMTIEDKSENKE"
     gene            complement(2044..2997)
                     /locus_tag="ACHJ8C_004142"
     CDS             complement(2044..2997)
                     /locus_tag="ACHJ8C_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO5553912.1"
                     /translation="MVTSIPLLLLILVALIFVKSAVKIVPQGNAWTVERFGKYTHTLS
                     PGLHFLIPFMDRIGQRINMMETVLDVPKQEVISKDNANVTIDAVCFIQVIDAAKAAYE
                     VDNLASAISNLVMTNIRTVVGGMNLDDMLSQRDSINSKLLTVVDYATDPWGIKVTRIE
                     IRDVKPPEELTKAMNAQMKAERTKRAQILEAEGIRQSQILKAEGEKQSQILKAEGERQ
                     SAFLQSEARERQAEAEARATKLVSDAIAEGDVQSVNYFIAQKYTEALQAIGTASNSKL
                     VMMPLDSSSLVSSVAGISELLKNVSQDTPKVDMKQAQQYLS"
     gene            complement(3024..3200)
                     /locus_tag="ACHJ8C_004143"
     CDS             complement(3024..3200)
                     /locus_tag="ACHJ8C_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553913.1"
                     /translation="MADFGSTKQTVTFEEWHELLMDYAELRGGNAADAEAWRGDYEAG
                     KTPVEAYCDEWGED"
     gene            complement(3193..3408)
                     /locus_tag="ACHJ8C_004144"
     CDS             complement(3193..3408)
                     /locus_tag="ACHJ8C_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO5553914.1"
                     /translation="MPKYQINATIKKPGGGPVKWTHYSDFPMTAEECRKNFSKEKEAG
                     KCFSVDVHISDFICKEIPPQNAGNTYG"
     gene            complement(3401..3853)
                     /locus_tag="ACHJ8C_004145"
     CDS             complement(3401..3853)
                     /locus_tag="ACHJ8C_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiF/GrlA family transcriptional regulator"
                     /protein_id="HFO5553915.1"
                     /translation="MARKLKINPVIPECVADLKGYPLYIIVAYWGLRNNKTLTTTDVS
                     RNFLITQQQSYDVLTYIYQEAQKQITATKRTLFNEHKRRISAFRILDIDKGILRTPKK
                     EIAYASYDNIPTVTMKSTRCEIQDSFTELRRWMCSRKTGDRVEVFLNA"
     gene            4991..6019
                     /gene="repA"
                     /locus_tag="ACHJ8C_004146"
     CDS             4991..6019
                     /gene="repA"
                     /locus_tag="ACHJ8C_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFO5553916.1"
                     /translation="MQLAVISKRHWSELSPEEQVRFWQDYEAGIESSFLVPQENKGGT
                     TKRRRGEHSTKPKCENPAWFRPDSYKALGGQLGHAYNRLVKKDPVTGQYTLRMHMSLH
                     PFYVLKRQSVGRKYKFRPEKQRLLDAIWVVLVSFCDRGLHTVGMSVSRLAEEISPKDS
                     KGNVIPETAVTVSRLSRLLAEQVCFGTLGTSEKTIWDRESRQRLPKYVWITETGWKML
                     GVDLVKLQEQQRKRLAESEIRLQLIKEGVIREGEEISVHSARKRWYAQRSLDAIKFRR
                     EKAAKRKRANRLAKLPYDEQRNEIARFILKRMPPDEAYWCTKERLEQLVARDLRQLEL
                     ALTASPPH"
     gene            complement(6117..6488)
                     /locus_tag="ACHJ8C_004147"
     CDS             complement(6117..6488)
                     /locus_tag="ACHJ8C_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5553917.1"
                     /translation="MAEYLQRFFVGGLEGLCFSLGRSPYARREAVGGTFNILSGAALA
                     AASEGIFVIIHSCFKDKYFPCGEATQGIKEQGLRIYCGFDLLRLLLSEVIRLRVFVNN
                     RRRFSSDTRLWPSKNCQSGTK"
     gene            7372..7524
                     /locus_tag="ACHJ8C_004148"
                     /pseudo
     CDS             7372..7524
                     /locus_tag="ACHJ8C_004148"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            7469..7591
                     /locus_tag="ACHJ8C_004149"
     CDS             7469..7591
                     /locus_tag="ACHJ8C_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5553918.1"
                     /translation="MVCCTLLIFTYLTRNRLCEVRLKDGNREVTASLAYESNGK"
     gene            7741..8337
                     /locus_tag="ACHJ8C_004150"
     CDS             7741..8337
                     /locus_tag="ACHJ8C_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO5553919.1"
                     /translation="MTAIIEASMQPKKTPVIVVKKRRVLVMPENPVVNEKPQEVQKSA
                     VNENKKVQKKDAVAEKTHKKQPQPWYLKKQITFPPKYPKEYFEKCFNKVRAVFPELWT
                     DEKKNLPLKSGILQDVEKYLADNPDVDLTIEEWNCAVQVMTFRWQYLQNCTVPGATRY
                     DLYGKPAGTVKKAHATYAQLVLDARKKASEKKQLKRKG"
     gene            complement(8355..8639)
                     /locus_tag="ACHJ8C_004151"
     CDS             complement(8355..8639)
                     /locus_tag="ACHJ8C_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO5553920.1"
                     /translation="MALDLKTPVIPGQKTEQTNADTARFINEATRKKSGATKVTNVRL
                     GDVYEDILEREALRTGQTKTAIMKAALAAYEHGLDENGKNHWILQSARLK"
     gene            complement(8713..9378)
                     /locus_tag="ACHJ8C_004152"
     CDS             complement(8713..9378)
                     /locus_tag="ACHJ8C_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HFO5553921.1"
                     /translation="MGRALLVASDKGGVGKSTTATNLASYLVNNSRTVIILKTDKNND
                     ILSWNEKRKQNGLLPVPVYEEYGDVSEVIKRLKKICEVLIIDCPGHDSKEFRSALTVA
                     DILLTLVKPSSDFEAETLTHVTEKVRTAQQINPDLQPWVLFTRINTTKPRHRKAAIDL
                     DKLLRENPVWIQPLRTRISDLDIFEAACNEGAGVHDVRRASSLSTAKAQIELLAQELG
                     INP"
BASE COUNT         2615 a         2065 c         2077 g         2785 t
ORIGIN      
        1 ttgaagataa ttgtcggtca atgcttttga ttttttttcg tatctcaatt ggattcgcct
       61 cagataagtt agcaatggat aaagccagtt cgatttgtaa aattttcctt gtttcgatga
      121 gattgatttt tacttgtggt atatcacgga agacaccaca gccggtaatt gcttttgaaa
      181 cgacacgttc tgtattcgct atgtttttct ttactgttcg aggggggaca taacgtgcac
      241 ggcgaagttt tctcagatta gcttttgctt gagtcatgta atgtgtccag agttataatt
      301 ttatttcttt gcatgattcc cctttacggc acacagctac acggttattt atcacattca
      361 ccactgtcac agttccgtcg tcgcattgag tgacgatgaa agggtattcg ttgatttttg
      421 tacagtgaga ttgttctcct gctattgcaa ccacaactgg gctaataaat aacgccagaa
      481 cagatgccag cttaataaaa gcactcattt ttgtatgccc tatataaata aatgctttct
      541 tcaaatcctg aatatactat ctgaatacag aagcggtcct gttctcctga agtaaggctt
      601 cattatttta tgggccattg gatgggtagt atgtacgggt attgatagaa tgaattttga
      661 atgtcctgtt tttattgcat tcagaagttc gtcaatttct ttttgagatg tcgttgcttt
      721 catcaacaag tgcctcaatc agtttattaa tacacgtccg ttctgccgga acctcccggc
      781 ctgtaatcgg gtcaaaaacg acatgatcgc tttcaacgta acgtaaacgg tgatagacat
      841 ctatacggag ttttgtttta gcagcttcac gttcaactcg agttaactct tctttttctc
      901 gtttgctgct gaaaaacaga aaccagatca ttgctgtgat aattactaaa aaagggacaa
      961 cggtgcctaa aatgcttacc atttctgcca tgccacccat aatagattga atctttcctg
     1021 tggctgtggt ggttgttact ggttcagcaa agtcagaacc agtgtttgat aaagatgtaa
     1081 ataaacgggc agataaacca aatagcacgc caaaaacgag aacttttatt atcgaaccgt
     1141 atacatttaa tagactgtat tcttcctctt taggcgattc aaagtttttt gccctttgtg
     1201 tctgctcttg tttagaacca gaaacacctc cgatgaataa gccaatacca ccagtaccta
     1261 aaccaatacc cccaccagta ccattccatt tacctgttgt tgtttcttta gctacaatag
     1321 ctgcaagaga aattccttca tttgcgtttc gcatagaatt gtcctcatac tattcctgtg
     1381 acacttaaca ccatcaataa aatatgtaat gcagtaattt tttttagcgt ttttttatag
     1441 acttcaataa aatccttatc cagaagcctg ataataaaaa tacatagtgc attcatcgct
     1501 gctcctgtaa gcggaattaa caacgaaaga tgcatgattt attccttgtt ttcactcttg
     1561 tcttcgatgg tcattacttt aagagttatt ccttgcatgt ctgtaacaat aactcttgtt
     1621 cccgcagggt agttttcatc gtgtaacgtc tcaacaggcc agaatgtatc accaataagt
     1681 aattgcccct tgccgttcac aatatcgtca ctgagagtaa attcttttcc tttaaaacgt
     1741 gtatgtcctg tgttcaccag gggtgtatct aatttcttat gttttttgtc atagacgaac
     1801 ttaattatcg acgtcagtat gcttattgac gcaaagaagc atatatttgc ttcttgtgat
     1861 ataggaagga aaaagtcaac aatggcactt gataatgcac caagactgat tacgagtaac
     1921 catcccgttc cggttataat ttcaagggca atacaaagaa gaaacagcgt aaaccagaac
     1981 atcgttaatc tccatgagat aaggacggtg attaaaacaa tattgtcacc gtccggtatc
     2041 tgtttaagac aaatactgtt gagcctgttt catatcaact ttgggagtat cctgtgatac
     2101 gttttttagc agttcactga tacctgcaac cgaactcacc aggctacttg aatcaagagg
     2161 catcataacc agtttactgt ttgatgcagt gccaattgcc tggagtgctt cagtgtattt
     2221 ttgcgcaata aagtaattaa cagactgaac atcaccttca gcgattgcat ccgacaccag
     2281 ttttgttgcg cgagcttccg cttctgcctg acgttcgcgt gcttcagact gaagaaatgc
     2341 tgattgccgt tccccttctg ctttcagtat ttgggactgt ttttcccctt ccgccttcag
     2401 aatttgtgac tggcgtatac cttccgcttc cagaatctgt gcacgcttag tacgttccgc
     2461 tttcatttgc gcgttcatcg ccttagtcag ttcttctggt ggtttaacat cccgaatttc
     2521 aatacgtgtg accttaattc cccacggatc agttgcataa tcgacaaccg ttaacagctt
     2581 agaattgatg ctgtcacgtt gagaaagcat atcgtcgaga ttcattccac caacgacagt
     2641 tcggatattg gtcatgacaa gatttgaaat agctgatgca agattatcga cttcataagc
     2701 tgccttcgct gcatcaatga cctggataaa acaaacagca tcgatagtta cgtttgcatt
     2761 gtctttcgaa ataacttcct gtttaggaac atccaggacg gtttccatca tgttgatccg
     2821 ttgacctatg cggtccataa acgggatcag gaagtgaaga ccgggggaaa gcgtatgggt
     2881 atatttacca aagcgttcca ctgtccaggc attaccttgc ggcacgattt tgacggcaga
     2941 tttaacgaat atcagcgcga ctaaaattaa taagagtaac ggaatagatg taactattgg
     3001 cgtaattata gcgtcaatca tgattaatcc tctccccatt catcacaata tgcttcaacc
     3061 ggagtttttc ctgcttcata atcaccgcgc catgcttccg catcggcggc atttccaccg
     3121 cgtaactctg cgtaatccat taatagctcg tgccattctt caaatgttac ggtttgttta
     3181 gtggaaccaa aatcagccat atgtatttcc tgcattttgt ggaggtatct ctttgcaaat
     3241 gaaatctgaa atatgaacat caacagaaaa acactttcct gcttcttttt cttttgagaa
     3301 gttttttctg cattcttcag cggtcatagg gaagtcgctg taatgagtcc atttcacggg
     3361 gccaccacct ggttttttga tggttgcgtt tatttggtac ttaggcattc aaaaatacct
     3421 ccactcgatc acctgtcttt cttgaacaca tccagcggcg cagttctgta aaactatcct
     3481 ggatctcaca acgggtactt ttcattgtga cagttggtat attgtcgtag ctggcatatg
     3541 caatctcttt tttgggtgtg cgcaatatgc ctttgtcaat atcaagaatc ctgaacgcgc
     3601 taattcttct tttgtgctcg ttaaatagtg ttcgtttagt tgctgttatc tgcttctgtg
     3661 cttcctggta aatgtaggta agcacatcat aagattgttg ttgtgtaata aggaaattac
     3721 ggctaacatc cgttgttgtg agcgttttat tatttcttaa tccccaataa gccacgatga
     3781 tatagagtgg atatcctttc aaatctgcca cacattcagg aatgacagga ttaattttta
     3841 acttacgcgc catattttct cccggttgtt tgtctgaaat agaaacatcc ccggaataca
     3901 gcgtcataag ctaattatga aaagttgcag ccactcacta caggctcttt atctgattgt
     3961 tcatcatcat gcatagctaa acccccttac ctggagtgga gactggcaaa tctggtgtac
     4021 ttctgcgttg cagcctgtac gacttgctca gggagagagt gagtgagatt aactcaacgc
     4081 gagttcacca aataaggggg tttaagtatg cagaatgata tttgcggata aagagtcgtt
     4141 acctgtgtag atggcgcggc acgtcactct gttacgtagc ccgttgctga cagatgccag
     4201 cggtgcttga aatgcacgaa taataacctc cttatgccag ggctttaggc aatgaccgct
     4261 gtccggggtt gttaaagagc atgagatgac aagcgttcta acgggcgtca aagagtgctc
     4321 attttccagg caatgagcac aaaaaacgcg atgaacgagc tttttacaat catctggata
     4381 gtattgtgtg tgcgacattt aaaacctgtc aatgccacaa gagcgatcta atgatctgga
     4441 taacatctaa aaaacagaat aatattctgt ttgttaaatg atgtacgatc taaaaatcta
     4501 gataatactg tttgtgtggt ttattcgatc gtgtgaatgg gtgtatgcag tcaaaaaaac
     4561 ttttttgaat atacaaaatt ctgaatttct gattttgtaa ggttttgtat ggggaacgtc
     4621 agtaaagaag gggcttcagt agctgttctg aaaacgattt tgaagcagtc acagatgtgt
     4681 gctgaatcaa aaaggaaacg agccaggctt gaacatcgtt gatcgattgc gcccatgctg
     4741 ctaaatttgc aggcaagttt tccgcgattt gtaaatgcag aaaacgaggt gctgaaattt
     4801 tgttcaagaa cgtaaatttc agataaagaa aacccccgta atctcgtgtt ttccaggcag
     4861 cgtagattag cgggggttgc cctcaaggcc gagccttgag ctgataacgt tttatttcag
     4921 gtatgcttgt tacctgaaat ggagtttaat tccatgagaa ggaaatatca actcttacag
     4981 tgcaagctaa gtgcagcttg ctgtgattag caaacgtcac tggtctgaac tatcaccgga
     5041 agagcaggtc cgtttctggc aggactacga agcgggcatt gaaagctcat tcctggtccc
     5101 ccaggaaaac aaaggcggaa caacaaaacg tcgccggggt gaacattcca ctaagccaaa
     5161 gtgtgagaat ccagcctggt tccgtcccga cagctataag gcgctgggcg gacagttggg
     5221 ccacgcttac aatcgtctcg ttaaaaaaga cccggtaact ggtcagtaca cactcagaat
     5281 gcacatgtcg ttgcatccat tttatgtact gaaacgtcag agtgtcggtc gcaaatataa
     5341 attccgaccg gagaaacagc ggctgcttga cgcaatatgg gttgtacttg tgagtttttg
     5401 tgatcgtgga ttgcacactg tcggcatgtc tgtctcccgc ctggcagaag aaataagtcc
     5461 aaaagacagt aagggaaacg ttattcctga aaccgcagtc acagtatcgc gcctttcccg
     5521 tctgctggca gagcaggttt gctttggcac gttgggaacg tcagaaaaga cgatatggga
     5581 tcgtgaatcc cgccagcgac tgccgaaata cgtctggatc accgaaactg gctggaaaat
     5641 gctgggagtt gatctggtga aacttcagga acagcaacgt aaacgccttg cagaaagtga
     5701 aatccgtctg caactgatta aagaaggcgt tatccgcgag ggcgaggaaa tctctgttca
     5761 ctcggctcgt aaacgctggt atgcgcagcg ttctctcgat gccatcaagt tccgacgaga
     5821 gaaagcagca aagcgcaaac gtgccaatcg ccttgcaaaa ctgccgtatg atgaacagcg
     5881 aaacgaaatt gcacggttta ttctgaaacg tatgccgccg gacgaagcgt actggtgcac
     5941 taaagaaaga ttagagcaac tggtagccag ggatctacgt cagcttgaac tggccctgac
     6001 agcttcgcca ccccactagt ttagctgcct gatacaatcg gcactctctt cagccccttc
     6061 ggggcttttc gtgttgcctg catttcatga aattcctcat ttttgacgac cgtttttcat
     6121 tttgtcccgc tctgacagtt ttttgatggc cacaatcggg tatcggagct aaaccgcctt
     6181 cggttattaa caaaaacccg caaacgaata acttcagaaa gcagcaaacg tagaagatca
     6241 aacccgcaat atattcttaa cccctgttct ttaatcccct gcgttgcttc gccgcaggga
     6301 aaatatttat ctttgaaaca actgtggata attacaaaaa tgccttcgct tgcagcggct
     6361 aacgccgcgc cgctcagaat attaaaagta cctcccaccg cttcgcggcg ggcatatgga
     6421 cttctgccta atgaaaaaca aagcccctcc aaccctccta caaaaaaacg ctgcaaatac
     6481 tcagccacag cgcccaaccc atcactgaaa agcgccgccg cccccgcccg aagggcggga
     6541 acaacatcgc ttttaataat ggatgttgta actaagaaat tacatggctg tcagtcttct
     6601 ggctggaagt accgagtaca cgctcgtaag cggccctgac ggcccgctaa cgcggagata
     6661 cgccccgact gcgggtaaac ccttgtcggg accactccga ccgcgcacaa aagctatttc
     6721 atggctgaaa gcgggtatgg cttagcagga tggggatggg taaggtgaaa cctatcaatc
     6781 agtaccggct tacgccgggc ttcggcggtt ttactccagt atcatatgta acaacggagt
     6841 gccgccttcc atgccgctgg cgcggcatca aaaaagaaag ccccccaatg acggggggaa
     6901 acagcgtttt gatcaaaata ataatgctag tgtgcttctt gcgtgccatt ccaagcacaa
     6961 actaaattta acgtttcgtt aactacaaag aaatccgatg agttaatcac ttgcgaaata
     7021 aaaatcatat agtaaattac tatatgattt tgcgttgttt cggagaatta aaaaatgcct
     7081 ttgattgaat acatcaacaa gttctatcgg gggaatcagg catcgttcgc caggctgacc
     7141 ggagttcaac ctgcgcaggt aacacagtgg atcaacaaag gattcatcgt ggtgaatcac
     7201 acactgtata gtccacgccg aaagctggga atttaattcc tggtcaatat ataatgctgt
     7261 tggcgcttga ggctttctgc ctcatggcga aaaggtggtt tgtatcttgt tgtgcggcag
     7321 aaagaagaaa gccccgtagt taatttttca ttaacccacg aggcgatccc aatgcttcaa
     7381 caacatcagg atagcctctt accgcgcgtt acgcaaggag aagaaggcca tgaaacagcc
     7441 gaaaaacgcc ctgacctggt gtttattaat cgtgtgctgc acattgttaa tattcacgta
     7501 tctgacccgg aatcgtttgt gcgaggtccg gctgaaagat ggaaacaggg aggttacggc
     7561 aagtctggct tacgaatcca acggtaagta gcaacccgga ggcgggcgaa agcccgcctt
     7621 tttggggatg atgtggttct ggcattaagc gcctttgcaa agggggtatt ctgtacggat
     7681 accccctttt tccatgtatt ttcaggatat taactgctga aaatggtata attagtcaat
     7741 atgacagcaa ttattgaggc gagtatgcag ccaaaaaaaa cacccgtgat tgtggtcaaa
     7801 aaacgccgcg ttctggtcat gccagaaaat cctgtggtga atgaaaagcc gcaggaagta
     7861 caaaaatcag cagttaatga aaacaagaaa gtacagaaga aagatgctgt agcagagaaa
     7921 acacataaaa agcagcctca gccctggtat ttgaaaaaac aaatcacttt tcccccaaaa
     7981 tatccgaaag aatattttga aaagtgtttc aataaagttc gtgctgtttt tcctgaacta
     8041 tggacagacg aaaaaaagaa cttgcccctg aaaagcggga ttctccagga cgttgagaaa
     8101 tacctggcgg ataacccgga tgtggatctg acgattgaag agtggaattg tgcggttcag
     8161 gtgatgacgt tccggtggca atatcttcag aattgtactg taccaggcgc aacacgttac
     8221 gacctttacg ggaaacctgc tggtacagta aaaaaagcac acgcaactta tgcgcaactg
     8281 gttctcgatg cccgtaagaa agccagcgag aagaaacaat taaaacgtaa gggataaagc
     8341 cccgaagggc tttatcattt cagcctggct gactgtagaa tccagtgatt ttttccgttt
     8401 tcgtccaggc cgtgttcgta agctgcaaga gccgctttca ttattgctgt ttttgtctgg
     8461 cctgttcgca gtgcttcccg ttccagaata tcttcgtaaa cgtccccaag ccggacattg
     8521 gtcactttag ttgctcccga ttttttgcgg gtagcttcat tgatgaatcg ggctgtgtct
     8581 gcattggttt gttctgtttt ttgtcctggt attaccggag ttttcaagtc aagagccatt
     8641 atatcacctt tcgtgtgtta cattagcttt actgtgtaaa gttaatgatg taaagctatc
     8701 gtattacatc cattagggat tgatgcctaa ctcttgcgcc aaaagctcta tttgtgcttt
     8761 agctgtagaa aggctggatg cgcgacgtac atcatgaacg ccagcgcctt cattacaggc
     8821 cgcttcaaaa atatctaaat ctgaaatacg tgtacgaagt ggctgtatcc acaccgggtt
     8881 ctcccggagt aacttgtcga gatcaatcgc tgcttttctg tggcgtggtt ttgtcgtgtt
     8941 aattctcgtg aaaagtaccc aaggttgcag gtctggattg atctgttgag ctgtgcgaac
     9001 tttttctgtt acatgagtca gcgtttctgc ttcgaaatct gaagacggct taacgagagt
     9061 aagcagaatg tcagctacag ttaaggcact gcgaaattcc ttgctgtcat gccctggaca
     9121 gtcaatgatc agcacttcac atattttttt caggcgctta atgacttctg atacatcgcc
     9181 atactcttca taaacaggaa ccggtaataa gccattctgc tttctttttt cgttccagct
     9241 aagaatatca ttgtttttat cggtcttcag aatgatgacc gttcgtgaat tatttaccag
     9301 ataagaagcc aggtttgttg ctgtggtgct ttttcctaca ccgcctttat ctgatgcgac
     9361 aagtaatgct ctccccatag aaacctcgga tatacagtgt aaaggtttat ttgtctttac
     9421 attgtattca ttatagttac gttgtaaaga tgtttgtgtc tttacaatgt gattgttttt
     9481 tgtgctttac attgtgtgta tgttacaggg taacttttca ggatgcgatt ttgcctggtg
     9541 tg
//
LOCUS       DAZFJL010000167         9499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFJL010000167 DAZFJL010000000
VERSION     DAZFJL010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.169"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..504)
                     /gene="mocA"
                     /locus_tag="ACHJ8C_004153"
     CDS             complement(<1..504)
                     /gene="mocA"
                     /locus_tag="ACHJ8C_004153"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO5553922.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYVNQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     EHYSVEIE"
     gene            608..1378
                     /gene="yqeC"
                     /locus_tag="ACHJ8C_004154"
     CDS             608..1378
                     /gene="yqeC"
                     /locus_tag="ACHJ8C_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO5553923.1"
                     /translation="MKSIIDPSALFIDLGAQKRPTVISIVGAGGKTSLLFWLAELFQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASFISPISFCFHCWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPAAPLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHNVEAIWLGDIQEHPAIARRFVN"
     gene            1426..3051
                     /gene="yqeB"
                     /locus_tag="ACHJ8C_004155"
     CDS             1426..3051
                     /gene="yqeB"
                     /locus_tag="ACHJ8C_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO5553924.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPTCSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3092..4024)
                     /gene="arcC"
                     /locus_tag="ACHJ8C_004156"
     CDS             complement(3092..4024)
                     /gene="arcC"
                     /locus_tag="ACHJ8C_004156"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO5553925.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLTPTKPIGSFFTEQEAELLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4072..5457)
                     /gene="hyuA"
                     /locus_tag="ACHJ8C_004157"
     CDS             complement(4072..5457)
                     /gene="hyuA"
                     /locus_tag="ACHJ8C_004157"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO5553926.1"
                     /translation="MRVLIKNGIVVNADGQAKQDLLIESGIVRQLGTDISPQLPCEEI
                     DASGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRQQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRISPERFVELTSAMPARLFGLWPQKGILAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5511..6722)
                     /locus_tag="ACHJ8C_004158"
     CDS             complement(5511..6722)
                     /locus_tag="ACHJ8C_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO5553927.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(6780..7976)
                     /gene="dpaL"
                     /locus_tag="ACHJ8C_004159"
     CDS             complement(6780..7976)
                     /gene="dpaL"
                     /locus_tag="ACHJ8C_004159"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO5553928.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYHP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NIGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8034..9221)
                     /gene="ygeW"
                     /locus_tag="ACHJ8C_004160"
     CDS             complement(8034..9221)
                     /gene="ygeW"
                     /locus_tag="ACHJ8C_004160"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO5553929.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT         2228 a         2316 c         2478 g         2477 t
ORIGIN      
        1 ttctatttcg acggaataat gttctcccat aaggagtgcc tgacgcatat tggtgacatt
       61 gggtcgctgg attgcctgca tcaggcatgg ttttgacact aaaatcggat ggccggggat
      121 gccattatgg agtggcagta ttgcgccatc atttcgtaac gaccagattt ttctaaaaat
      181 atcgatggtg agggttggca tatctccgtg ggtgagaaaa caatgttctg tttgcaccgc
      241 gggtactgcg gcctttactg atgttagtaa accctgcgca taatctgggt tgtggataat
      301 agtgatattg ctctggttca cataacgttc gtgcagttca ttaccacgat agccggtgac
      361 taaaataatt cggctacaaa actgcaacgc atttttgata cttgtatcaa gaattgttcc
      421 ctgttgccaa ggtaacatca ttttccattg ccccattctt gatgataatc ctgcggcggt
      481 aattatacag tcgatggctg acatagtgat tccattattt acaggagcta ataacgcctg
      541 ataattgcgt tacttcctga tggattacgt tgagggtaat ttatcccaga gaggtccata
      601 aagacgcgtg aaaagtataa ttgacccatc ggcattattc attgatttag gcgcgcagaa
      661 acgtcccact gtgatttcta ttgttggtgc tggggggaaa accagcttgc ttttttggct
      721 ggcagaactg ttccaggcga gtggcaggcg tgtattaatc actacgacga cacatatgtt
      781 tatgccaaca tctcactggc ccgtggtttt ctgtcgtgat cccgccatgc ttcctcatgc
      841 gtcttttata tcccccattt cattttgttt tcactgctgg aaagcgaacc agggaaaagt
      901 gcagggattt acgccagaag cgattgatgc actggtacaa cgaccagagt gtgacgtaat
      961 tctcattgag gcagatggct cgcgtggaat gccgttaaaa gcgcctgatg agcacgaacc
     1021 ttgcatacct aaaagcagtt gctgcgtgat tgctgtgatg ggaggacata ttttgggtgc
     1081 gaaagtgagc acagaaaatg tccatcgctg gtcgcagttt gctgatatta ctgggttaac
     1141 acctgctgca cccttgcaac tgagcgatct cgttgcgctg gttcgccacc ctcagggggc
     1201 gtttaaaaac gtaccacaag gttgtcggcg agtctggttc attaaccgtt tttctcaatg
     1261 tgagaatgcg attgcgcaaa gcgagctcct tcaaccgctg caacaacaca acgtagaggc
     1321 aatctggctg ggcgatatac aagaacatcc tgcaatcgcg cgcagatttg tgaattagcg
     1381 gcccgggtat tccgggttac tgaaaggata tccattgagg ttggtatgaa tattttcaca
     1441 gaggctgcaa aactcgaaga gcaaaattgt ccgtttgcga tggcgcaaat tgttgatagc
     1501 cgaggctcaa ccccccgcca ttctgcacaa atgttagtgc gcgccgatgg ttctatcgtc
     1561 ggtacaattg gtggcggaat ggttgaacgg aaggtgattg aagagtcgct tcaggcattg
     1621 caggaacgta agccgcgatt attccatgga cgtatggctc gtaacggtgc ggatgctgtc
     1681 gggtcagatt gtggaggtgc aatgtcagta tttatcagcg tccatggtat gcgtccacgt
     1741 ctggtgttga ttggtgcggg gcatgtcaac cgggcgatag cccagagtgc ggcgctatta
     1801 ggatttgata tcgccgttgc tgatatttat cgcgaaagcc tcaatcctga actattccca
     1861 ccatcaacca cgcttctcca tgctgagtcg tttggtgcgg cagtggaagc actggatatt
     1921 cgccctgata attttgtcct gattgccacg aataatcagg atcgtgaagc cctcgacaaa
     1981 ctcattgaac aacccattgc atggctgggg ttgctggcaa gtcgccgcaa ggttcagctt
     2041 ttcctgcgcc aattgcgtga gaaaggcgtg gctgaagaac atattgcccg tttacatgcg
     2101 cccgttggtt acaacatagg tgcggaaacg ccgcaggaga tcgccatcag cgtgctggca
     2161 gaaatattac aggtgaaaaa taacgcgccg ggtgggctga tgatgaagcc ttctcatcct
     2221 tccggacacc agctggtggt gattcgtggt gcgggggata tcgccagtgg tgtagcgcta
     2281 cgtctgtatc atgcgggttt taaagtgatc atgttggaag tggaaaaacc gacagtgatt
     2341 cgttgtaccg tggcgtttgc ccaggccgtg ttcgatggcg aaatgacggt cgaaggcgtc
     2401 accgcccgcc tggcaaccag ctctgcggaa gcgatgaaac ttaccgaacg cggatttatc
     2461 cctgtgatgg tagatcccac ctgttcattg cttgatgaac tgaaaccgct ttgcgtggtg
     2521 gacgctattc tggcgaaaca gaatttggga acgcgggcag atatggcacc agtaacaatc
     2581 gcgcttgggc cgggctttac tgcagggaag gattgtcatg cggtaattga aacaaatcgc
     2641 gggcactggc tcggtcaggt gatttactct ggttgtgcgc aggagaatac cggtgttccc
     2701 ggcaatatta tggggcatac cacccgacgg gtgatccgtg ctcctgctgc aggcattatg
     2761 cgatccaacg tgaaattagg cgatctggtg aaagagggcg atgtcattgc ctggattggt
     2821 gagcatgaaa ttaaagcacc gttgacgggg atggtgcgtg gcttgttgaa cgacggactg
     2881 gccgtggtcg gtggttttaa aattggtgat atcgatcctc gtggtgaaac ggctgatttc
     2941 accagcgttt ctgataaagc ccgggcgatt ggcggcggcg tacttgaggc gttaatgatg
     3001 ttgatgcatc agggcgttaa agcgacaaaa gaagtgctgg aagtggctta aatgaaaatg
     3061 gccggatggt attacccatc cggcctttaa attactgaca aatcaccgtt ccggttttgc
     3121 cttcaatccc ttctttcgct ttgctcaata cggtaatcag tgcttcacga cctgcgcgag
     3181 agcgagcaaa cgaggcggca gcttccactt taggcagcat agaacctttc gcaaaatgac
     3241 cttcttcgat aaagcgttca gcatcactta acgacaggcg atcgagccac tgttcattct
     3301 cttttccgaa gttaatggcc actttttcta ccgcagtcag aatgatcagc atatcggcat
     3361 caatcatttc tgctaaacgg gcgctggccc agtctttatc gataaccgcg ctggcaccac
     3421 gcagatggtt accttcacga ataacaggga taccaccacc gccaacggtg atcaccacct
     3481 gtccggcatc taccagagct ttaaccgttt ctttttcaat gatatcaacc ggttttggcg
     3541 aagcaacgac acggcgatag ccgcgacccg catcttcttt cagagtgtaa ccctgttttg
     3601 tcagtagctc tgcttcttgc tcggtaaaga acgaaccgat cggcttggtc ggggtgagga
     3661 atgccggatc gttagcatcg acttcgacct gggtaaccag cgttgctaca ggtttattaa
     3721 tgccacgaga aagcagttct tctcttaagg cgttttgtag atcgtaacca atataaccct
     3781 ggcttaaggc aacacagaca gacatcggca gcattggcga gtgcgcttca gtttttgcgg
     3841 cagcttcaaa ggcctggtta atcatgccca cctgtggccc gttgccatga gtgacgatga
     3901 cctcatgtcc ctgggcaatt aaatcaacaa ttgcctgaga agtaattttt accgctttca
     3961 tttgtccggc caggtcatcg cccagcgcat tcccgcccag ggcgagaaca attttcttac
     4021 tcatttttcc tcactcatca ttcttgattg tgtatagatc cctggcagtg gttagagcac
     4081 gggagggaca aacggtttgc gtcgcaggaa acgacctcgc ccggctttgc ctgtaaaggt
     4141 gccgtcgcag aaaatcgttt caccacggga taaggttctg acaatcgcgc cctgacaggt
     4201 aaacccctcc catggcgagt agtcagcgtt gtcgtggaga tggcgatgct gaatttgttg
     4261 gctctgacgt gggtcgataa tcaccacgtc accatcggaa ccgggcgcta atatcccttt
     4321 ttgcggccac aggccaaaca gcctggcggg cattgcactg gttaactcaa caaaacgctc
     4381 cggtgagata cgtcccgtca tcacgccact ggagaacagc agttgcatgc ggttctccac
     4441 accgggtaag ccatttgggc agcggctgaa atcgccttta gaaatttgct ggcgctgggc
     4501 catcgagaag gtgcagtgat cggttgccac cacgtcaatc gcaccatcgc tgatgccaca
     4561 ccacagtttg tcctgctcgc gtacgttacg cagcggtggg ctaagaatga acttcatacc
     4621 atcttctgta tcgtaactgc gttcgtccaa caggagatat tgtgggcagg tttcaaccca
     4681 gactggctgg tgattcgcac gggcaagacg cagataatcc agacctaagc cgttagacag
     4741 gtgcacgata tagagcgggg cgttaccggc aatttgtgcc aggttaatca tgcgggcgat
     4801 ggcttccgct tcgcattcca gaggtcgact caaggcatga taacgcggcg cggttaaccc
     4861 tgcggcgata aactccgccc gcttgctggc gatagctgcg tcattttccg ggtgtacggt
     4921 ggtcagcgcg ccggactcat gcaagcggcg taatgcctgc aacacttcat catcgttgag
     4981 tttatattga taggttaaat agagtttaaa actgctcagt ccttcctcaa ccatcatcgg
     5041 aatttcgtcg aggatagcgt gattaatatg ttgaatgaca ccgtgaaagc tgtagtcgat
     5101 gactgctttg tgggcggcat aaccgcgata aacttccagt tgatggcgta accggcagcc
     5161 atttgggcca aatcccatat ggtcaataat ggttgttgta ccgccacacg cagcagcgcg
     5221 ggtaccggta aaaaaatcat cacaactgcg cgcgatgccg acatcaatat tgaaatgcgt
     5281 atggacatcc acgccgccag ggaaaacgta acagccagag gcatcaattt cttcgcaagg
     5341 gagttgcggc gaaatatcgg tacccaactg gcgaacaatc ccgctttcaa tcagcaaatc
     5401 ctgcttcgcc tgtccatctg cattaacaac aatgccgttt tttatcaata cgcgcatagc
     5461 aaactccaaa aaccctccgg taaaccggag ggggaataag tgaagagtaa ttattcggtt
     5521 gccagccagc ttaatgggat tgcagcgtac atagcggcac aagttaccag gtgagatttc
     5581 caggtttttt cgtttggcgc gtgcgcttca ggttctttac cagggccaaa accgatgacc
     5641 ggaatgccgt ggcgacccat gatggatacg ccgttagttg agaaggtcca cttatcaacg
     5701 acaggcgctt taccaaacag cccttcataa gcattcacca gtgctttaac ggtgaagtga
     5761 tcttcttcca ctttccaggt cgggaagtag cattcggttg ggtaaaccag gccagtccag
     5821 gacgggcggt cgtagttgta catagaaaca accgcgttag ctttctgtac tgcaggtaga
     5881 gcgcggatct cgtccagcgc gccttcccag gtttcgcccc aggtcagacg acggtcgatt
     5941 gaaactgcgc agctgtctgc tacagcgcaa cgacttgggg aggtgaagaa gatttcagaa
     6001 acagtcagcg tacctttgcc gaggaattcg tcatagccca gacgttgaga cagttcctgt
     6061 aattcgccaa gaattggacc cattttgaaa atggcgttat caccacgttc tggtgccgaa
     6121 ccgtggcagc taacaccctg aacatcaatg cgaatttcca tacgaccgcg ttgaccacgg
     6181 tatacctggc agtcggttgg ttcggtactg accacaaatt ccgggcgaat gccggattgt
     6241 tcaataatgt attgccagca cagaccgtcg cagtcttctt cctgcacagt accggtgacc
     6301 agcagggtat attcatcttc cagaccgaga tctttaatga tttttccggc atataccata
     6361 gacgccatgc cgccttcctg gtcggaagta ccacgaccac cgatcagctc gtcggtttcc
     6421 atgccttcat acggatcgaa gtcccagttt ttgatgttgc caatgccgac tgtatcgata
     6481 tgagcgtcca ttgccaccag acgcgggccg tggccgatat aaccgagaac attgcccatt
     6541 gggtcgattt caactttatc gaagccgact ttttccatct cttctttaat acgatgtact
     6601 acgcgtttct cgtcgcagct ttcactggga atagcaacca tatcgcgcag gaagcgagtc
     6661 atgtctgcct ggtaatcttt tgctttttca agaatcagtt tgaatggaat attcttagcc
     6721 attatttttc tccagatttt ttactccccg ttttgcaggg agcagttcca atagggtgat
     6781 taaggtgcta cagcgtgttt gccttcccag acaacttcgc ggtagtgctt cacgtcggtg
     6841 tcgccttcag tgctgataac cagcaccacg gcatctttgt tcagcgccag tttttccatc
     6901 aggctttgac gttgcgggtg ataatgaacc gctgcgagaa cgcccaaacc gacagcgccg
     6961 gattcaccgg agatgatgcg cgggtcgttg ccatacggat tacccagcac gcgcatacct
     7021 aatgcggcaa cgctgtcctg gcaggagatg aattgggtgg cgcagttacg taggatttcc
     7081 cagcccagtg ggttaggttc gccgcaggcc aggcccgcca tgatggtggc catatcaccg
     7141 ccaatgttga cgatgtcgcc tttgacgccg gagcgataaa tacagtcagc tttgtcaggt
     7201 tcaacaataa tgctgtgcag attttgcggg ctatagacgt cgaccagata acccagcaca
     7261 ccaccggcca ttgcaccgac accggcttgc agcagaacgt gtgtcggggt gacgcccatt
     7321 tcacgcattt gctcgacggc ttcatccgcc agtgttgcgt agccttgcat gatccaggtt
     7381 gggattttgg tgtaaccttc ccatgccgtg tcctgtacca cttcccagcc gtgctgctgc
     7441 gcgtgttgca tggtcaggcg aacggtatcg tcatagttca tatccgtgac gatgcactcg
     7501 gcaccgaggt ttagaatggc gtcaacgcgt tcctgagcag aacctttcgg catgtaaatc
     7561 accgcattct gtccgagttg ctgtgctgcc catgccacac cgcgcccgtg gttgccgtcg
     7621 gtggtagtcg cgaaagtcat tttttcgccg atggcatttt tcaagtgctc aaatgacagc
     7681 gtttcgatat caagatgata tttttcacac aataattgag cgatggcgta cgcaccgccg
     7741 agcattttga aggcgttcag accgaatcgt tttgattcgt ctttgacgag aattttcttt
     7801 acaccaaaca ggtttgcgag atcgtccagc gcacaaagcg gtgttgggtg ataaccagca
     7861 attttctggt ggaactggcg tgccagtttt gcctggcttt gcgaaaaaag cggtgatgtt
     7921 tcgccgttga aaaatttgtt atcggcgata tcaatcttca atgagaaaac ggacatatcc
     7981 tatccttttg gcctctggaa atagagggcc gaccagctga tcggctcgag gggttatttc
     8041 acgcgttctt gcgcttcttt cagcagttgc tcgagcagtg cacctggttt ggcgtattta
     8101 cgggacagga tcatcgcggc gatgatataa ggcttccagc tggcttcttt gtaggtagcg
     8161 atacggtatt tttcgaatac gccttcagtc acttcacctt ctttgcagga tacgccgctg
     8221 atatcagctg gcaggcagtg catgtacagg gcttcgccat cacgggtcag ttccatcatc
     8281 tcttcagtac aatgccagtc tttgtgttgc gcgttctgtg ccagacactg tttttccagt
     8341 gcttttaagc cttcgtgatc gttcgcacgc agcaattcag tacgctcttc catcactttg
     8401 taaggtgccc atgacttcgg ataaacgatg tctgcgtctt tgaaggcttc ttccatgctg
     8461 gtgacctgac ggaagctacc accggaggct ttagcattgt ttttagcaac ttcaacgaca
     8521 tccgggatca ggtcgtagcc ttccggatgg gccagggtga catccatacc gaagcgggtc
     8581 atcagaccta tgatgccttg tggtacagag agcggtttgc catagcttgg ggagtaggcc
     8641 caggtcatgg cgattttttt gcctttcagg ttttccagcg aaccaaagtg ttcacgcaac
     8701 catgccaggt cagccataga ctgagtcggg tggtcaatat cgcattgcag gttcactaaa
     8761 gccggacgct gcggcagcac accctgcttg tagccgtcat caagtgcagc gccaacttca
     8821 cgcatataag cgttgcctgc gcccagatac atatcgtcgc gaataccgat agcgtcagcg
     8881 cagaaggaga tcatattggc ggtttcacgc acggtttcgc catgagcgat ttgtgattta
     8941 ccttcgtcaa gatcttgttg tgcaaggccg agcaggttaa gcgcggaagc ataagagaag
     9001 cgggtacggg tggaattgtc gcggaatacg gaaataccta atccactatt aaagactttg
     9061 gttgagatgt tttcagcacg cagtgctttt aacgcggcgg caacgtccag tacttgtttc
     9121 agttcatctg gcgtctgttc ccacgttaac aaaaaatctt tctcgtgaag gtgagaggtc
     9181 agcgaattga tatccttaat cagctcatta acagttttca tcataaagtc ccggtagtgg
     9241 aatgacaggc aaagtggttt atttcactgt gacttgccaa agtgattggt gttttcacag
     9301 ctatacctgg gactaacaat aagtgtgcca gaattatcct cggggctgca aaagactaat
     9361 ttgacggata atgtgtgggg ttgatcacaa attgatgcca gcttccggtt gtttgtaaaa
     9421 tacgaaaacg tttggctttt tttatcgcgt tatttatttg ctgctaagta tcagaaataa
     9481 gataaaggga caggcgatc
//
LOCUS       DAZFJL010000168         9165 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFJL010000168 DAZFJL010000000
VERSION     DAZFJL010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.170"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(29..355)
                     /locus_tag="ACHJ8C_004161"
     CDS             complement(29..355)
                     /locus_tag="ACHJ8C_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO5553930.1"
                     /translation="MKRTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVERAKPGEYVQGING
                     KWVRKF"
     gene            complement(363..548)
                     /locus_tag="ACHJ8C_004162"
     CDS             complement(363..548)
                     /locus_tag="ACHJ8C_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO5553931.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(545..3184)
                     /locus_tag="ACHJ8C_004163"
     CDS             complement(545..3184)
                     /locus_tag="ACHJ8C_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO5553932.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLVGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQAEQSPAAPKTLAQWQAMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVAAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     ELSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLSLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWGRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTRINLNNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3392..4381
                     /gene="ldhA"
                     /locus_tag="ACHJ8C_004164"
     CDS             3392..4381
                     /gene="ldhA"
                     /locus_tag="ACHJ8C_004164"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO5553933.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4492..4914
                     /gene="hslJ"
                     /locus_tag="ACHJ8C_004165"
     CDS             4492..4914
                     /gene="hslJ"
                     /locus_tag="ACHJ8C_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO5553934.1"
                     /translation="MKKVAALVALSLLMAGCVSSDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4911..5177)
                     /locus_tag="ACHJ8C_004166"
     CDS             complement(4911..5177)
                     /locus_tag="ACHJ8C_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO5553935.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5451..8975
                     /gene="nifJ"
                     /locus_tag="ACHJ8C_004167"
     CDS             5451..8975
                     /gene="nifJ"
                     /locus_tag="ACHJ8C_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO5553936.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILELMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRNVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSAAEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLYSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2280 a         2398 c         2249 g         2238 t
ORIGIN      
        1 gcgttatccc gatattgcgg ttttgctatt aaaattttcg cacccattta ccgtttatcc
       61 cttgtacata ttccccgggt ttggcccgtt ctacgagctt ttgcccggcg agtttcgcga
      121 tatcatccac cgacacatta ttttgctttg ccagttgttg atagctcgct ttacgttcgg
      181 cattgatgtc ggttactaat ttctcagttt cagcatctgt tttcagcgca accagataac
      241 cataaaatgt ttcgcctacc cgcccctgag ttctggcttc atccagagtc aatgccatta
      301 cattgctgct tactaagcca acaagaaatg cacaaagaag taatgttctt ttcatcatca
      361 cctcaaaaaa gatcgctacg ggtctcaagc agctcttcga catctttgtc tgccttgatg
      421 atgatctcat gctcaatttt aacgttcata ttgatagtga tcggttcctt aggtgcagcg
      481 acttcaatgc gaggcgtaca gccaaccagc ataaaagatg acgtcaacgc agccagtaaa
      541 attttcattg tttttcctca cactcttttc cttgcggaca gtcatttccc ggcagacgtg
      601 cgttctgctc cagccatgcc tggagattat cgccaaagcg taaactgcgc cacagcgtaa
      661 aaatattctc ttcatgatgg taattaaggt ttaccgtacc gcttttacca tcaacgcgac
      721 tggtacctgt aatgttggcc tgcatggtta ataaaccgag attatttaaa ttaattcttg
      781 tcgatgaacg gctaatttcc atatagcgca accagttaat tgctgaaccc gcggtcatat
      841 tgtctttcac caccgcatct gcggtgtctt tatccagccg caaggtcatc ggcccgctat
      901 tagccagcca gccgtctttc actatccatt tttcgttgtt tagccataac ggcaatgcac
      961 cactaaatgc cccggacatg gcgaattgtt tcggattgac ggcgctaacc agttcgctgg
     1021 aggaaagatt attcagccgc aacagcgccg ggtcatgttg cggcatacgt aattgtttca
     1081 tcagcacgtt accgcctaac acatcgacgc taacatcagt caacagcaat ggttcttctt
     1141 cagtccacgg ataacgacct tgcaaatcag ccgtaatatt tttcgctgtc accagattaa
     1201 tcacttcggc aattcgcaac gtaacgggac cgcgagtccc cagatgccag gctccatcgg
     1261 caaaacggaa aggcaggaca aaatcgacac cgttaacctg attatctggc atccaggcac
     1321 taccgccttt caacacgccg tgtcctcccg cgcggaatcc ttgttcgggc gctgctgaaa
     1381 atgcaacctg agcatacagt tcaccatcgc gtaagttcat cttccagtcg ggtggcacca
     1441 acggctggaa tacagtcagt gattgtttag gccaccaggc gttaccgcgc agacgaatac
     1501 cgtcccagcg accatttacc cgaaccgggc caatctcacc agcatgtaaa tcgcctttaa
     1561 agaggaaata cgtcggatcg cgcccatcga cactaaattt taaggttgat ggcggtaaca
     1621 cactgccgcc agtgaacagc gtttgcccgg cgtccagtga cagagcgccg ctgaaggagg
     1681 gatgctgtgc gtcacgtccc cagacgacag gcttgtcgag aattaatcgc ggcttttcta
     1741 ccgtcatcgt accgtattgt aactggtcga aaccggtgga gagatcggtc agcgtaatcg
     1801 tgctgtcatg ccactcacct tttcctgcga catcccaggt ggcattcatc ggtgtaaaac
     1861 tcccttttcc ccagtagcgc cactgccagc ggccagcgtc agggagaaaa tcattcgcca
     1921 gcccatccat atgcagcacg aaatcgccca gttcattttc atgcgcctgc aaaatggcct
     1981 gcaaacgtcc gtcaacacca cgttgggtga cttttacacc cgctaaaggc cagcggattt
     2041 catcgatgtc cagcgaatca atgactcttc cctttgaacg aagtaacgcg cctggctcaa
     2101 aggtcagcgt tgggtcagac agacttccac ttagctgcgc aggtaaacgg gcatataaaa
     2161 tgaggtccgc ctgtttcgct tcaccggtca gctgcagaga cagctggctg ttatccatgc
     2221 ttaattttcc tgggccaaaa tttaacaccg cgttgccttt acccgcttgc ccctgggtca
     2281 gcacactcag tcgcccgctg accagagcgt tctcgagccc tgcctgccag ttgtcgactt
     2341 taacacccag tcggccactc aaaggaaaac ctgcatacgg ccagctccag cgaccatcgc
     2401 ttacggtcaa ttgttgacga gtaatttgcc acggcaaatc gagcaacgga tcgccgttat
     2461 cccgtgccag cacaatcaat tgcccgctat tttcctgcca gtccagctcg gcatccacca
     2521 gtgacagttc ctgcggcaag tttaacgtcg cagtagcatg accacttacc ggaagtccat
     2581 ccggcacgag cggcatagta aattccccca ccagttttac cggcggctga ttttcaaacg
     2641 cggcgacatc cagttcgctg actgtaagtt gttgcccttt cagctggcct tgaaatttaa
     2701 ctttttcgcc ctgataacgc agttgctgga tatcggaggt taatgcgaga gagagttttc
     2761 cctgccattc ctgccaggga gaaaaaatca gtttatcgat attgatccag gtgttaggca
     2821 acatggcctg ccactgcgcg agggtttttg gagcggctgg cgattgttcc gcctgcggca
     2881 atttcgccag acaagcagaa tcaagttcta ccgtgccgac gttcaataac cagcggctgg
     2941 gatgtgaaag gctggcgttg gtgatatgcg caagctgaca atctcccacc agataacgga
     3001 gatcggggat gattaaacct ttacgcgtaa tgcgtgggct ttcatctaat gcaatacgtg
     3061 tcccgagcgg tagccagatg cccaccagcg tgggaaccca cagcccgagc gtcatcagca
     3121 gcgtcaacgg cacaagaata atcagtaata acagcgcgag aacggcttta tatttaccca
     3181 gcatgggtag ttaatatcct gatttagcga aaaattaagc attcaatacg ggtattgtgg
     3241 catgtttaac cgttcagttg aaggttgcgc ctacactaag catagttgtt gatgaatttt
     3301 tcaatatcgc catgtctttc aattatattt gaaattttgt aaaatatttt tagtagctta
     3361 aatgtgattc aacatcactg gagaaagtct tatgaaactc gccgtttata gcacaaaaca
     3421 gtacgacaag aagtacctgc aacaggtgaa cgagtccttt ggctttgagc tggaattttt
     3481 tgactttctg ctgacggaaa aaaccgctaa aactgccaat ggctgcgaag cggtatgtat
     3541 tttcgtaaac gatgacggca gccgcccggt gctggaagag ctgaaaaagc acggcgttaa
     3601 atatatcgcc ctgcgctgtg ccggtttcaa taacgtcgac cttgacgcgg caaaagaact
     3661 ggggctcaaa gtagtccgtg ttccagccta tgatccagag gccgttgctg aacacgccat
     3721 cggtatgatg atgacgctga accgccgtat tcaccgcgcg tatcagcgta cccgtgacgc
     3781 taacttctct ctggaaggtc tgaccggctt tactatgtat ggcaaaacgg caggcgttat
     3841 cggtaccggt aaaatcggtg tggcgatgct gcgcattctg aaaggttttg gtatgcgtct
     3901 gctggcgttc gatccatatc caagtgcggc ggcgctggaa ctcggtgtgg agtatgtcga
     3961 tctgccaacc ctgttctctg aatcagacgt tatctctctg cactgcccgc tgacaccgga
     4021 aaactaccat ctgttgaacg aagccgcctt cgatcagatg aaaaatggcg tgatgatcgt
     4081 caataccagt cgcggtgcat tgattgattc tcaggcggca attgaagcgc tgaaaaatca
     4141 gaaaattggt tcgttgggta tggacgtgta tgagaacgaa cgcgatctgt tctttgaaga
     4201 taaatccaac gacgtgatcc aggatgacgt attccgtcgc ttgtctgcct gccacaacgt
     4261 gttgtttacc gggcaccagg cattcctgac agcagaagct ctgaccagta tttctcagac
     4321 tacgctgcaa aacttaagca atctggaaaa aggcgaaacc tgcccgaacg aactggttta
     4381 atcttcccgc tcccctgcat tccaggggag cttattcaga taatccccaa agacctttca
     4441 tcctctattc ttaaaatagt cctgaatcag aaacagtaat tgagaaccac aatgaagaaa
     4501 gtagccgcgc ttgttgcgct aagcctgctg atggcgggat gtgtaagtag tgacaaaatt
     4561 gctgtaacgc cagaacagct acagcatcat cgctttgtgc tggaaagcgt aaacggtaag
     4621 cccgtgacca gcgataaaaa tccgccagaa atcagctttg gtgaaaagat gatgatttcc
     4681 ggcagcatgt gtaaccgctt tagcggtgaa ggcaaactgt ctaatggtga actgaccgcc
     4741 aaagggctgg caatgacccg tatgatgtgc gctaacccgc agcttaatga actcgataac
     4801 accattagcg aaatgctgaa agaaggtgca caagtggatc tgaccgcgaa ccagttaacg
     4861 ctggcgaccg cgaaacagac attaacttat aagctggcgg atttaatgaa ttaatagctg
     4921 ccacagctcc cggcggcaag tgactgttcg ctacagcgtt tgccgttggg taatgcacac
     4981 atcccaatcg ccgtaccatc cagttggcgg gcaacagaaa gcgaaccgcc gatcattgca
     5041 caatttgctt ctccactact ggacatcgac gcttttaaac ctggcgctac gtgcgccgca
     5101 gtcgcctgct gaacaggttc actactacac gccgacaaca ataaagcggc acaccctacc
     5161 caaaacgctg ctcgcatctt ttcttcctct gatcttcaag ccaaacgaca ccgccataaa
     5221 taataggcag cacagagggc ggcgtcgaga gctgtcctgc gcgttgcccc gcatttttac
     5281 ttttttatgg ctattttttt gccctctgtt tgatcaaaac attcattacg ctgatgtggg
     5341 ggacacaaaa gcgaaaatgc agaagaaagc catttgctaa aattgaacga ttactactgg
     5401 gcgcgcagca atttcgtgcg cccctcattt gcgcaatgta agggtgtcat atgattacta
     5461 ttgacggtaa tggcgcggtt gcttcggtgg cgtttcgcac cagtgaagtt atcgccatct
     5521 accctattac ccccagttcc acgatggcag aacaggctga tgcatgggcc ggaaacggct
     5581 tgaaaaacgt ttggggagac acaccacgcg tggttgaaat gcagtcggaa gcgggtgcta
     5641 tcgctaccgt gcatggtgct ttgcaaactg gtgccctctc aacatcgttt acgtcatcgc
     5701 agggtttgct gctgatgatc ccaacgctgt acaaactggc aggcgaactc acaccgtttg
     5761 tcctgcatgt tgcggcacgt accgttgcca cacatgcact ctctattttt ggcgatcatt
     5821 ccgacgttat ggcagtgcgc caaacgggtt gcgcgatgtt gtgtgcagca aacgtccagg
     5881 aagcgcaaga ctttgctctc atttcgcaca ttgcgacgct gaaaagtcgc gtgccattta
     5941 ttcatttctt tgatggtttc cgcacatctc acgaaatcaa taaaattgtc ccgctggccg
     6001 atgacacgat tcttgaactg atgccgcagg cagaaattga tgctcatcgc gcccgggcac
     6061 tcaacccgga acatccggtg atccgcggta cgtctgccaa tcctgacact tatttccagt
     6121 ctcgcgaagc caccaaccca tggtacaacg cggtctatga ccatgttgaa caggcgatga
     6181 atgatttcgc tgccgcgaca ggtcgtcagt atcagccgtt tgagtattac gggcatccgc
     6241 aagcggaacg ggtgattatc ctgatgggct cagccattgg cacatgtgaa gaagtggttg
     6301 atgaattgct gacccgtggc gaaaaagtcg gcgtgctgaa agttcgcctg taccgcccct
     6361 tctccgccaa acacttgctg caagctctgc cgggatccgt gcgcaacgtg gcggtactgg
     6421 acagaaccaa agaacccggt gcccaggcag aaccgctcta tctggatgtg atgaccgcac
     6481 tggcagaagc ctttaataat ggcgagcgcg aaaccctgcc ccgtgtcatt ggtgggcgct
     6541 atggtctttc atccaaagaa tttggcccgg actgtgtact ggcggtattt gccgagctca
     6601 acgcggctaa accgaaagcg cgctttacgg ttggtattta cgatgatgta accaatctgt
     6661 cactgccgtt gccggaaaac accctgccaa actcggcgaa actggaagcc ttgttttatg
     6721 gccttggtag tgatggcagc gtttccgcga ccaaaaacaa tatcaagatt atcggtaatt
     6781 ccacgccgtg gtacgcacag ggctattttg tttacgattc caaaaaggcg ggcggcctga
     6841 cggtttctca ccttcgcgtg agcgaacagc cgattcgttc cgcttatctc atttcccagg
     6901 ctgattttgt tggctgccac cagttgcagt ttatcgataa atatcagatg gctgagcgtt
     6961 taaaacctgg cggcattttc ctgctcaaca cgccgtacag cgcagctgaa gtgtggtcgc
     7021 gcttgccgca ggaagttcag gccgtgctaa accagaaaaa agcgcgcttc tatgtgatta
     7081 acgcggcgaa aatcgcccgc gaatgtggcc tggcggcccg tattaatacc gtcatgcaga
     7141 tggctttttt ccatctgacg cagattctgc ctggcgatag tgccctcgca gaattgcagg
     7201 gtgcgattgc caaaagttac agtagcaaag gccaggatct ggtggaacgc aactggcagg
     7261 ctctggcgct ggcgcgtgaa tccgtagaag aagttccact gcagccagtt aatccgcata
     7321 gcgccaatcg accgccagtg gtttccgatg ccgctcctga tttcgtgaaa accgtaaccg
     7381 ctgcgatgct cgccgggctt ggcgacgccc tccccgtttc ggcgctgccg ccagacggca
     7441 cctggccgat gggcaccacg cgctgggaaa aacgcaatat cgccgaagag atccccatct
     7501 ggaaagagga actctgtacc caatgtaacc actgtgtcgc cgcttgccca cactcagcta
     7561 ttcgcgcaaa agtggtaccg cctgaagcga tggaaaacgc ccctgccagc ctgtattcgc
     7621 tggatgtgaa atcgcgtgat atgcgcgggc agaaatatgt cttgcaggtg gctccggaag
     7681 attgcaccgg ttgtaacctg tgcgtcgaag tttgcccggc gaaagaccgt cagaatccag
     7741 agattaaagc catcaatatg atgtcgcgcc tggaacatgt cgaagaagag aaaatcaatt
     7801 acgatttctt cctcaacctg ccagaaatcg accgtagcaa actggaacgt attgatattc
     7861 gtacatcgca gctgattaca ccgctgtttg aatattccgg tgcttgctcc ggttgtggcg
     7921 agacgccgta tattaaattg ctgactcagc tctatggcga ccggatgttg atcgctaatg
     7981 ccactggctg ttcttcaata tacggcggta acctgccttc aacaccatat accaccgatg
     8041 ccaatggtcg tggaccggca tgggcgaact cgctgtttga agataacgcc gaatttggcc
     8101 ttggtttccg cctgacggtc gatcaacacc gtgtccgcgt gctgcgtctg ctcgatcaat
     8161 ttgccgataa aatcccggcg gaattactga ctgcgttgaa atcagacgcc acgccagagg
     8221 ttcgtcgtga acaggttgca gctttacgcc agcaactcaa cgatgttgcc gaagcacatg
     8281 aactgctgcg tgacgcagat gcactggtgg aaaaatcaat ctggctgatt ggtggcgatg
     8341 gctgggctta cgatatcggc tttggcggtc tggatcatgt attgagtttg acggaaaacg
     8401 tcaacattct ggtgctggat acgcaatgct attccaacac cggtggtcag gcatcgaaag
     8461 cgacaccgct gggtgcagta actaagtttg gcgagcatgg taaacgtaaa gcgcgtaaag
     8521 atcttggcgt cagtatgatg atgtacggtc atgtttatgt ggcgcagatt tctctcggcg
     8581 cgcagctgaa ccagacggtg aaagcgattc aggaagcgga agcgtatccg gggccatcgc
     8641 tgatcatcgc ttatagccca tgtgaagagc atggttacga tctggcactc agccacgatc
     8701 agatgcgcca actcactgct accggcttct ggccgttata tcgcttcgat cctcgtcgtg
     8761 ccgatgaagg caaactgccg ctggcgctgg attcacgccc gccgtcagaa gcactggaag
     8821 aaacgttact tcacgagcag cgtttccgtc gtctgaattc gcagcagcca gaagtggcag
     8881 aacagttatg gaaagatgct gcagctgatt tgcaaaaacg ctatgacttc ctggcacaaa
     8941 tggccggaaa agcggaaaaa agcaacaccg attaaatgct ctggataagg attatccaat
     9001 tctaaaaaaa agcccggacg actgttcggg cttgtcttct tatatgttgg aaaatcagta
     9061 gcaatgcaat ggcccaacag aaaataaaat ctgtgacaat aaaggcatat aacccgcgca
     9121 gaataacgca tagaaaatta aaagtataat ttttattttg tatgc
//
LOCUS       DAZFJL010000169         8608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFJL010000169 DAZFJL010000000
VERSION     DAZFJL010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.171"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(157..1068)
                     /gene="gsiD"
                     /locus_tag="ACHJ8C_004168"
     CDS             complement(157..1068)
                     /gene="gsiD"
                     /locus_tag="ACHJ8C_004168"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO5553937.1"
                     /translation="MRLFNWRRQTVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1071..1991)
                     /gene="gsiC"
                     /locus_tag="ACHJ8C_004169"
     CDS             complement(1071..1991)
                     /gene="gsiC"
                     /locus_tag="ACHJ8C_004169"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO5553938.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2009..3547)
                     /gene="gsiB"
                     /locus_tag="ACHJ8C_004170"
     CDS             complement(2009..3547)
                     /gene="gsiB"
                     /locus_tag="ACHJ8C_004170"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO5553939.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKNVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFVGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3567..5438)
                     /gene="gsiA"
                     /locus_tag="ACHJ8C_004171"
     CDS             complement(3567..5438)
                     /gene="gsiA"
                     /locus_tag="ACHJ8C_004171"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO5553940.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQEQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSILEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAVVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5425..6390)
                     /gene="iaaA"
                     /locus_tag="ACHJ8C_004172"
     CDS             complement(5425..6390)
                     /gene="iaaA"
                     /locus_tag="ACHJ8C_004172"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO5553941.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLV
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6594..7829
                     /gene="moeA"
                     /locus_tag="ACHJ8C_004173"
     CDS             6594..7829
                     /gene="moeA"
                     /locus_tag="ACHJ8C_004173"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO5553942.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLSVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEARSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            7829..8578
                     /gene="moeB"
                     /locus_tag="ACHJ8C_004174"
     CDS             7829..8578
                     /gene="moeB"
                     /locus_tag="ACHJ8C_004174"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO5553943.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVEVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
BASE COUNT         2041 a         2450 c         2195 g         1922 t
ORIGIN      
        1 aactgtctga acgctgtgcc gctgcaaaat agtcccttgc tgcggctttt taaccaaatt
       61 acccgattct acaaaatcag cagaaagtta caacacttgc ttagggcaat aatttcctgt
      121 gaggtgatta ccctttcaag caatattcaa acgtaactat cctttaattt tcggatccag
      181 cgcatcgcgt aaaccatcgc ccaacaaatt gaacgccagt acggtcagaa aaatagccag
      241 ggccggaaaa acagcgacat gcggcgcgat aaccatatcc gctcgtgcct cattgagcat
      301 tgctccccac tctggtgtcg gcggctgcgc accgaggccg agaaatgaca ggctggcggc
      361 agagataatc gaggtgccaa tgcgcatggt gaaaaacacc acgatagaag agacggtccc
      421 aggcaagata tgacgcaaca aaatggtcat atcgctggca ccaatactgc gtgctgactc
      481 gataaaggtt tgctgtttca acaccagcgt gttgccgcgc accagccggg caaacgcggg
      541 gatggaaaaa atggcgactg cgataatcac gttagcaatg ccgcttccca acaccgcaac
      601 aacagcgatc gccagtaaaa tacccgggaa ggcaaacagc acatcgcaaa tacgcatgat
      661 cagccgatcc caccagcctt cataatatcc agccagcaag cccagcaacg tcccgatcgc
      721 cgcaccgata aacacggcaa acacgcccgc cgccagcgag atttgcgcac caaccaggac
      781 acggctgaaa atgtcgcgcc ccagtgaatc gacaccaaac cagtgctgca aagaaggtcc
      841 gttattcaga ttgtcataat caaaataatt ttcggcgtca tagggagcga tccagcgtgc
      901 aaaaatggcc accacaatca ataaaataac gaacaatgcg gcggtcatcg ccatatgctg
      961 acggcgaaat cgtcgccaga attcatgcca cggtgtacgt acctggtcag gtttgaccag
     1021 tggcatggcg tttaacaccg tctgacgtcg ccagttaaat agtcgcatcc ttacttgtac
     1081 ctgatagccg ggttaatggc ggcgtaaagc acatccacca ctaagttgat aagaataaat
     1141 tccagcgaga aaagcagaat ttccgcctga atcaccgggt aatcgcgcat ttctacggag
     1201 tcaacgagta agcgtccaag tccgggccag ttgaaaactt tctccaccac gatggaacca
     1261 ccgagcaaga agccaaactg taagcccatc atggtcacta ccgggatcat cgcgttgcgt
     1321 agcccgtgtt tgaggacaac ccaggtttcg ctcacccctt tcgccctcgc ggtacgcata
     1381 taatcttcgc ttaaaacgtc gacaaacgac gcgcgggtaa agcgcgccat cacggcggcc
     1441 actgccgcgc cgagcgtcag ggagggtaaa atgtagtgct gccaactgtc tgctcccacg
     1501 gtaggcagcc agcccagttc aacggagaat acctgaatta aaagcatccc cagagcaaat
     1561 gccggaaacg agatccccga caccgcaatg gtcatactca atcgatccgg ccaacggtta
     1621 cgccagacgg cggcgataat tcccgccgcc ataccaaata tcaccgccca gaccatactg
     1681 gttatggtca gccacagcgt tggcataaag cggctggcaa tctcatcggc aaccggacga
     1741 cgcgacacca tcgacaggcc aaaatccccc tgcacagcat tgctgatata gtgccagaac
     1801 tggtgatata gcggctgatc caaccccagc tgctgacgca ccagttctat aacctgcgca
     1861 tcagcttcgg gcccggcaat taatcgcgcc ggatcgccgg gcagcatgtg gacaaataaa
     1921 aacaccagca ccgagacgat aaacagcgtc ggaatcaacc ccagtaagcg tttgataacg
     1981 taattaagca ttccactccc tgcgttgctt attgcaaatc cgcgtcttca aagctgaagc
     2041 cggtgtctgg catgatccaa aaaccggtca ggtttttact gtgtgccgac accagttttt
     2101 ctaccaccag cgggatccac ggcgactctt gccagatgat atcctgcgcc gccttataca
     2161 agcgggtctt ttccgccgga tcattagttt tcagtgcctg agccaggaag tcatccacct
     2221 gtttattgct gtaaaacgcg gtattaaaca gcgttggtgg ccagttctgc gaggcaaaca
     2281 gcggcgatag agcccagtcg gcttcgccgg ttgaagccga ccagccagtg tagaacatcc
     2341 gcacgccgct ctctttttgc cctttacctt caacttctgc cgcccgctgt ccggcatcca
     2401 tcgcagtcac ctgggcttta atcccaacct gcgctaactg ctgctgggta aattgcagca
     2461 ctttctgcgc ggtgctgtgg ttatgtgacg accacagcgt ggtactgaaa ccgttgggat
     2521 atcccgcctc tttcagtaat tcgcgcgctt tcactggatc gtaaggccac ggtttataac
     2581 tttgcgcgta ggcgatactc ggcggtacca caccagtagc tggcgttgca tagcccgcga
     2641 aggccacttt caccagcgcc gggcggttaa tggcgtaatt cagcgcctca cggaccttcg
     2701 ggttatcgaa cggcttttgc gtcacgttca tactgatata acgctgcata attgacggac
     2761 tggccatcaa ctcgatattt ttgtttttct ccagcagtgc ggcctgctcg tagggaatgg
     2821 ggaaagcaaa ctgcgcttca ccggtttgca gcattgccgc gcgggtgttg ttatccgcca
     2881 ccggacgcca ggttatgctg tccagtttgg gcaatcctgg ctgccagtaa cccacgaatt
     2941 ttttcacctt cacaaaatcg gtctgattcc aggtatccag ttcatacggc ccggttccca
     3001 ccggatgaaa accaatctcc ttgccatatt tttccagcgc tgccggtgaa atcatcgcgg
     3061 tcgccggatg ggcaagaata ttaataaacg ctgagaatgg ctgtttgagg gtaatcttta
     3121 ccgttgtcgg atcgatcgct tccgttttgg caatattctt atacaggtta tagcgtttaa
     3181 gatgattcgc cggatcgctg gcccggtcca gattcgcttt caccgccgct gcgttgaaat
     3241 cggtgccatc ctggaattta atgccttccc gcaatttcac ggtgtaagta aggccgtcat
     3301 cggaaacggt ataactctcc gccagcacgt ttttcagttt catctcttta tccagaccga
     3361 acagcccctg gtaaaacgat ttcgctacgg cctgagataa cgtgtcattt gcgtcatacg
     3421 gatcgagcgt ggtgaagttc gagcccaccg ccaccaccac atttttggca gcgaatgcac
     3481 aagatgccat caacgctgtc gcaatgccca gcgccactaa cccactacgg tgtacagctc
     3541 ttgccatttt attctccgcc tgaatgttat ctacgcatga atgcgtattc tgattgtggt
     3601 tgtgcgacgt aatgccccgg cccgacgcat tgcaacgaga cgactgcaac ttcttcgcca
     3661 cgcagatgaa tattgctggg aagatcgtcc gacagcagta cacgctgcgg tcgttgtcgg
     3721 gacggttcag cgaccggaac tgccgccagt aatttacgcg tataagggtg ctgcggattt
     3781 tcaaagaccg cgcgccgtgg accaatttca acaatttgcc cgagatacat caccgccaca
     3841 cgatgactaa tccgctcaac cacggccata tcgtgggaga taaacagata cgcaatgccg
     3901 aaatcacgct ggagatcgag caacaagttg ataatctgcc cgcgaataga aacatccagc
     3961 gccgagacgg cttcgtcggc aatgattact tttggattca atgccaacgc gcgagcaatg
     4021 cagatgcgct ggcgctgacc gccggaaaac tcatgagggt aacgccaggc atgttccggt
     4081 aacagaccca cgcgctccag caaccacgca acgcgtgcag ccgcttcctt acctggcaat
     4141 aaaccgtgta cgcgcagcgg ttcgagaatc gaatcaccga tggtctgacg tgggtccagc
     4201 gaagcgtaag ggtcctgaaa aataaactga atatcgcggc gcaatgcctg aagtttgccg
     4261 ggtgacaagg tatcgattcg ctgaccgtta aagataattt cgccgccttg cgattcgacc
     4321 aggcgcagca acgcccgccc ggtagtagat ttaccgctgc cagactctcc caccagcgat
     4381 agcgtttcac caggccagag atcaaaactg actttctcaa cggcatgcac ttcccgcgtt
     4441 acgcgattca acaaaccgct gcgcaaaggg aaacgggtga ccagattacg cacccgtaaa
     4501 acaggttcgc catccaccac cgttttctgc tcgatgggtg gctcctgttt cgctggatgt
     4561 tcaagcgata tcaatgggaa acgtcgggga taatctaacc ctttcatcgc accaagttgc
     4621 ggaacagcag ctaacagcgc acgggtataa ggatgttgcg gtgcatgaaa aatctgttcg
     4681 acgctacccg tttccaccgc ctcgccctga tacatcacca gtacccgatc ggcaatctct
     4741 gccaccacgc ccatatcgtg agtgataaag ataacgccca tcgacatctc tttttgcaat
     4801 actttgatta attgcaggat ctgcgcctga atagtgacat ccagcgcggt ggttggctca
     4861 tcggcaatca gcaccgccgg gcggcatgac agcgccatcg caatcatcac tcgctggcgc
     4921 atcccgccag agagttgatg cggataacgt gaaagaatgg tttgtgcctc cggaatgcgt
     4981 acctgatcca gcatccgctt cgcctcgacc atcgcttctt cacgactggc gttctgatgc
     5041 agacgaatag attcagcaat ctgttcaccc acagtaaata ccgggttcag cgatgtcatc
     5101 ggctcctgaa atatcatcgc catatccgca ccgcgcacat ggcgcatttg tgcagcgctc
     5161 tgctcgctaa gttcaattac ttcgcgactg cgccgctgca acagcatttt atcgcactgc
     5221 actaaaccgc ccgcctgttc caacaggcgc atcaatgcca gcgcagtcac tgacttaccg
     5281 gagccggatt cgccaacaat tgccagcgtc tcaccacgtt gcaggctaaa agagagattg
     5341 cggaccgcag ctattttctg ctgctcctgc ataaaggcaa tattcagatt ttcaaccgcc
     5401 agcacatcac cggcatcaag ttcatcactg tgtggcaacg gtgtcccctt tttcacggta
     5461 gataccggtg gttggcgtat cgcctgcgta gccccaggcg cgatacattc cttcggtgtt
     5521 aaacggtagc gcgacattcc cttcatggtc gatagcgatt aagccaccgc taccgccaag
     5581 cgcagggagt ttttccatta ctacccgctc gcaggcttcc gcgagactta atccgccgta
     5641 atccattaac gcggcgatat catatgccgc cagcgcgcgg atgaagactt cgcccgtacc
     5701 ggtacaggaa accgccacac tggcgttatt ggcatagcat ccggccccca ctaagggact
     5761 atcgccaact cgtccgggta atttattggt cattccgccc gtggacgtgg ctgccgccag
     5821 attgccgtct aaatccaacg ccacggcccc tacggtgccc attttctgtt tttcatccag
     5881 tggcgcaccg ctatggtcga ggactgttgc cccttcctcg cgcgctgcca ttagttgttc
     5941 ataacgcaaa ggtgtggaga aaatctccgg cgagacgcgc tccatgccat gagcaaacgc
     6001 aaaattttct gccccttcgc caatcatcat cacatgcggg ctttgctcca tcaccagtcg
     6061 ggcggcaaga accggattac gcagatgact aacgcccgcc accgcaccgg ctttcagggt
     6121 gttaccatcc atcacacagg cgtccagttc atgggtttca tcacgcgtaa agacagcgcc
     6181 aattccggcg ttaaacagtg gacactcttc cagcagacgc accgcttccg tcaccacatc
     6241 cagcgcactt tcgcccgcta ccagcatttt ctgcccggtt tcaacaatgg cagacaacgc
     6301 ctcgatgtag cgtaattcct gttgcagact catctgcgcg cggctaattg cacctgcgcc
     6361 accatgaatt gcaatgactg ctttgcccat agtattcgtc cattaagggt ggctcgccac
     6421 ttttgctata aatatcagaa tttttatgaa tgactcattc aatttttgaa tatagaaaga
     6481 tggatatggc atgtaaaggc aggagccagc gctacgcccg aggtggcata cagtctgtgg
     6541 cgtttttctg acataatagg caaattcgat tttgcctccg caggagtgtt ttcatggaat
     6601 ttaccaccgg attgatgtcg ctcgacaccg cgcttaatga gatgctttct cgcgttaccc
     6661 cactgaccgc ccaggaaacg ctgccactgg ttcagtgttt tggccgtatt ctggcgagcg
     6721 atgtcgtttc gccactggat gtcccggggt ttgataactc cgctatggac ggctacgcgg
     6781 tgcgtttagc cgatattgcc tccgggcaac cgctgtccgt tgccggtaaa tcctttgccg
     6841 gtcagccgta ccatggtgaa tggcctgcgg gtacctgcat tcgtattatg accggtgcgc
     6901 cggtgccgga aggctgcgaa gcggtggtga tgcaggagca gactgaacaa acggacaatg
     6961 gcgtgcgttt tactgctgaa gcgcgtagcg ggcaaaatat tcgccgtcgc ggtgaagata
     7021 tctctgcagg tgcagttgtt ttcccggcgg ggactcgcct gactaccgct gaactgccag
     7081 tgattgcttc gctagggatt gccgaagttc cggtgattcg taaagtgcgt gtagcgcttt
     7141 tttctaccgg tgatgaactc cagttgcccg gtcagccgct gggcgacggc caaatctacg
     7201 ataccaaccg tctcgccgta cacctgatgt tagaacagtt gggatgcgag gtaatcaact
     7261 tagggattat ccgcgacgat ccccatgccc tgcgcgccgc atttattgaa gccgacagcc
     7321 aggcggatgt ggtgatcagt tccggcggtg tttcagtggg tgaagcggat tacaccaaaa
     7381 cgattcttga agagctgggg gagatcgcct tctggaaact ggcgattaaa ccgggtaaac
     7441 cgttcgcgtt cggtaaactt tctaatagtt ggttctgcgg cctgccgggc aacccggttt
     7501 cagcaacgct gaccttctat caactggtac agcctttgct ggcaaaacta agcggcaaca
     7561 ccgccagcgg cctgcccgcg cgccagcgtg tacgcacagc gtcccgcctg aaaaaaacgc
     7621 caggacgcct tgatttccag cgcggcgtgc tgcaacgcaa cgccgatggc gaactggaag
     7681 tgacgaccac cggacatcag ggttcacata tatttagctc ctttagcctc ggcaactgct
     7741 ttatcgtgct ggaacgcgat cgcggcaatg tggaagtggg cgaatgggtg gaagtagaac
     7801 cgtttaacgc gttgttcgga ggcctgtaat ggcggaactc agcgatcagg agatgctgcg
     7861 ctacaaccgg caaatcattc tgcgcggttt tgattttgac ggtcaggagg cgctgaagga
     7921 ttctcgcgtg ttggtggtgg ggcttggtgg cctcggctgc gcagcttcgc agtatctggc
     7981 aagcgccggt gtcggcaacc tgacgctgct cgacttcgac acggtttcgc tctcgaatct
     8041 gcaacgccag actctgcaca gtgatgccac ggtcgggcaa ccgaaggtgg aatccgcccg
     8101 tgacgccctg acgcggatca acccacatat cgcgattacg ccagtcaatg cactgctgga
     8161 tgacgcagaa cttgctgcga tgattgcaaa acacgatctg gtgctcgact gtacggataa
     8221 cgttgaagta cgtaatcaac tgaacgcagg ctgttttgcc gcgaaggtgc cgctggtttc
     8281 cggcgcggca attcgcatgg aaggtcaaat cacggtcttc acttatcagg acggcgaacc
     8341 gtgctatcgc tgccttagcc gtttgtttgg cgagaacgcg ttaacctgcg tagaagcagg
     8401 cgtgatggca ccgttgatcg gcgtaattgg ttcgttgcag gcaatggaag cgatcaaact
     8461 gctggcaggt tatggcaaac cagccagcgg gaaaatcgtc atgtacgatg cgatgacctg
     8521 ccagtttcgt gaaatgaaac tgatgcgcaa tccggggtgt gaagtgtgcg ggcagtaatt
     8581 gccacctcca ggccggatga gacgcgac
//
LOCUS       DAZFJL010000170         8348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZFJL010000170 DAZFJL010000000
VERSION     DAZFJL010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.172"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            307..1116
                     /gene="pduF"
                     /locus_tag="ACHJ8C_004175"
     CDS             307..1116
                     /gene="pduF"
                     /locus_tag="ACHJ8C_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFO5553944.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCVAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNIVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWFAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKEISE"
     gene            1319..2233
                     /gene="pocR"
                     /locus_tag="ACHJ8C_004176"
     CDS             1319..2233
                     /gene="pocR"
                     /locus_tag="ACHJ8C_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein PocR"
                     /protein_id="HFO5553945.1"
                     /translation="MISAATLNSELINKIAQDFAQATSLAVVVVNIHGDEISELFNFT
                     PFCQMMRQHPQLSTRCRMSDRCGGLEASKSDQPCIYRCHAGLTDFSIPLVIAGHLVGF
                     VLCGQVRLRNDDVDLVDILNVDDCWQADPELLNEFRKVPEMDYSRVMASADLLKLIVE
                     NCLKKQLNFVVIKENKSQSDSLRQTRAPGPHDNKMKKALRYIDAHLSDDLRLEDVASH
                     VYLSPYYFSKLFKKYHGIGFNAWVNQQRMASARELLCHSDWSIASIARNLGFSQTSYF
                     CKVFRQTYQVTPQVYRQQVSDNAAMETV"
     regulatory      2325..2500
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2718..3155
                     /locus_tag="ACHJ8C_004177"
     CDS             2718..3155
                     /locus_tag="ACHJ8C_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFO5553946.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCLVGEDWQQDFQ
                     PFDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLNGPA
                     GGAVALIHYLNEICHARETAVNDAFCRTLTRGSLAIPQCHLLS"
     gene            3289..3834
                     /gene="cobU"
                     /locus_tag="ACHJ8C_004178"
     CDS             3289..3834
                     /gene="cobU"
                     /locus_tag="ACHJ8C_004178"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO5553947.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WNYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRINQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            3831..4574
                     /gene="cobS"
                     /locus_tag="ACHJ8C_004179"
     CDS             3831..4574
                     /gene="cobS"
                     /locus_tag="ACHJ8C_004179"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO5553948.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELTLRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            4586..5665
                     /gene="cobT"
                     /locus_tag="ACHJ8C_004180"
     CDS             4586..5665
                     /gene="cobT"
                     /locus_tag="ACHJ8C_004180"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO5553949.1"
                     /translation="MQILADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGKLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTVIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGWDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            5727..6653
                     /gene="ldtA"
                     /locus_tag="ACHJ8C_004181"
     CDS             5727..6653
                     /gene="ldtA"
                     /locus_tag="ACHJ8C_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO5553950.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGGRLVGQSLTATVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVNINRNGF"
     gene            6827..7792
                     /locus_tag="ACHJ8C_004182"
     CDS             6827..7792
                     /locus_tag="ACHJ8C_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO5553951.1"
                     /translation="MTKQNKNSRWQGYDPRYIYSLVVRRYFADMKTKIEIAEELGVSR
                     FKVARLIDEAIEQDYVRFIFPKQQAMDEEIANNLRKKFHLEDAIVLSVAESWTTQEEL
                     NHKLGEVTAEYLMQSLHEGMKVGIAWGRVLSSTVSKLSKLPPLDVVQLSGVHPGIEFS
                     QGPIDLIHKIAAISQGKAHPMYVPMWVDDEELAARLAGDPAVLDTQQYYSQLDVVITG
                     IGDWKSGSSSLCKIFPDTWCEALFQQDIAADVCISLVSSEGKILHSPIERLGFGISTD
                     QLQKAKKVIGVAGGEEKYEGILASLKSGLLNVLITDFDTAIKLLD"
     gene            complement(7844..8302)
                     /locus_tag="ACHJ8C_004183"
     CDS             complement(7844..8302)
                     /locus_tag="ACHJ8C_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO5553952.1"
                     /translation="MKFEILVNDVDNSISDYQTAITFAGTQLLEKGYITAEYIDACLE
                     REKSYPTGLMMANGQGIAIPHADYTLVNVNSISIVRFANDITFGQMEDADLTVDCSIM
                     FNLAFATSDQHMSILRRLFTLFQDNAFVETCRNLPATRVAKYVEDTLAAS"
BASE COUNT         2082 a         1729 c         2180 g         2357 t
ORIGIN      
        1 gtaatatttc gttgagtttg gttcgaagga aggaactgtg atatttgttt caattttatt
       61 atgtcatgat ggcaattttt tattgtaaaa gtttggtgga aattattact ttcaacactg
      121 attaacgctg tgttgcatga ttcggcaatg acaataattt gttatgttag cacgattttt
      181 acacgagtct gtcatgcatg ttggcaatat tttctgcaga gcaatttttt atagttgcgt
      241 tcggtataca caacaatcag cgcatagttg gctgataaag aaaaactccg taaagaaggt
      301 gtcactatga atgattcact caaggcgcaa tgcgttgccg agtttttggg caccggactg
      361 ttcctctttt ttggcattgg ttgtttgtgc gccctgaaag ttgccggggc aagtctggga
      421 ctgtgggaaa tttgtatcat ttgggggtta gggatttcgc ttgctgttta ccttacggca
      481 ggtatttccg gcgctcatct caacccggca gttaccattg ctctgtggct gtttgcctct
      541 ttcccagccc gtaaagtgct gccattttgt gtggcgcaat tagcgggtgc ctttggtggt
      601 gcggttctgg catattctct ctacagcagt ttgtttaccg attttgaatc tgctcacaat
      661 atagtgcgtg gtagcgcaga aagtttacaa ctcgccagta tcttcagtac ttatccagca
      721 gcggcgatta atgtatggca agctgcgttg gtcgaagtgg tcataacgtc catgttaatg
      781 gggttgatta tggcgttgac cgatgacggt aatggcgtac ccaaaggacc gcttgcgcct
      841 ttactcattg gtattctggt tgctgttatt ggtgcttcta ccggaccatt aaccggtttt
      901 gccatgaatc cagcgcgtga ttttggacct aaaattttca cctggtttgc gggttggggg
      961 gaaattgcga tgactggtgg acgcaatatt ccttatttca ttgtacccat tattgcaccg
     1021 atcattgggg catgtgttgg tgcggcgatt tatcgctatc tcattgcaaa gaatttacct
     1081 gtcaatagtg taacgcctaa agaaatcagc gaataacgaa ataatctggc agttataaac
     1141 gtttattttt ttactgtttg tgatctgctt tccgaactta ttaaaaagtc tcccgcttca
     1201 cggaggcttt tgttattgtc ctttgtgctt ctcgtcagta aatcatcaag gatgatgtga
     1261 agattaaatg taaaacaatt gttttgctat aacaataaat taactgggag gttttatcat
     1321 gatttctgcg gcgacactta attcagaact cattaataaa atcgcgcagg attttgcgca
     1381 ggcgaccagc cttgcggttg tcgtggtcaa tattcacggc gatgaaattt ccgagctgtt
     1441 taacttcact ccattttgtc agatgatgcg gcaacaccct cagctgagca ctcgatgtcg
     1501 catgagcgat cgttgtgggg gtctggaagc ctctaaatcc gatcagcctt gtatttatcg
     1561 ctgtcatgcc ggattaaccg atttctctat tccactggta atcgccgggc atttagttgg
     1621 ttttgtattg tgcgggcaag ttcgtctgcg taatgatgat gtcgatttgg tcgatattct
     1681 gaatgtcgat gattgctggc aggcagatcc cgaattactt aatgaatttc gcaaagtccc
     1741 tgagatggat tactcaagag ttatggcctc tgcggatttg ttgaagttga ttgtagaaaa
     1801 ttgcctaaaa aaacaactca attttgtggt tattaaagag aacaaatctc agtcagattc
     1861 gttgcgccag actcgtgcgc ctggtcccca cgacaataaa atgaaaaaag ctttacgtta
     1921 tattgatgcg catttatcag acgatttgcg tctggaagat gtcgcctcgc acgtttatct
     1981 aagcccttat tatttcagta agttatttaa gaaataccat ggaatcgggt ttaatgcgtg
     2041 ggtaaaccaa caacggatgg ccagcgctcg tgagttactg tgccatagtg actggagcat
     2101 cgccagcatt gcgcgtaatt taggtttttc gcaaacaagt tatttttgca aagttttccg
     2161 tcagacttat caggttacgc cgcaggtgta tcgacagcaa gtgagtgata atgcggcgat
     2221 ggaaacggta tagagtaaat gccccaaaaa aagaggacgg tttaaataaa ctctggaatg
     2281 aataattaac gttatttccc tgttatggga aaagtgaaat cagctcataa catacgctga
     2341 caggtgtggc acatttgtgc ctgggaagag ggtgaaaatc cctcgcagcc cccgctgctg
     2401 taatgctgac aaacccgaaa atgccactga tcgcgagatt gggaaggtcg ggagaggatg
     2461 acgctaagcc agaagaccag ccagtcagtc aataccaaca acttcggtgg gaagtgggtt
     2521 gcccggaagc gtccggtaga gtacaggcgc gtctttatac ccctaccacc ctgaaaggat
     2581 cagggtatgg cggcatcatt tagttctttt atcgcagtgc cgacaggcac cagggctgca
     2641 cttaaggtgt ggcaatcttg tttcatggct tatcaaccag accgccatta caggaaatta
     2701 agtggagggc gaggggcatg aaaacgataa aacccgaatc gatagctctt ttttgcctct
     2761 tatctggaga tgtggatctg gcgaaacatc tggcggaaat attgccaata acctgtttgg
     2821 ttggtgagga ctggcagcaa gatttccagc cttttgacgg tgggcctgtg caatcgttat
     2881 cagatgcgta tagcgattat tcagtgctgc tattgatagc agctgaaagc gagatatccg
     2941 taccagaatg gctcgctcat tctgagggaa cgacggtaat tttgattgca gaacacaatg
     3001 cccgtttgct gaatggtccg gctggtgggg ctgtagcatt aatccattat ctgaatgaga
     3061 tctgtcatgc cagagaaacc gctgtcaatg acgcattttg tcggacgtta acacgaggaa
     3121 gtctggctat tccgcaatgc catcttcttt cgtaatggat tgagaatgct ggatttttaa
     3181 ttatattacg cgcagtatta atcgcgtaaa tgttttgtgg tcagcaacgg tgggggagcc
     3241 attgttctgt tcaatgaata ccatcataca attgaatcag gagtcggtat gatgattttg
     3301 gtaacggggg gcgcacggag cgggaagagt cgccacgcag aggcgcttat tggggactct
     3361 tcacaggttc tgtatatcgc tacctcgcaa atccttgatg atgagatggc tgcacggata
     3421 gaacatcatc ggcaaggccg cccggagcac tggcgcacag tggagcgctg gcaacatctt
     3481 gatgaattaa ttcatgcaga cattaacccg aatgaggctg tgttgctgga atgcgttacc
     3541 acaatggtga ctaatctgtt gtttgattat ggcggcgata aagaccctga tgaatggaat
     3601 tatcaggcga tggaacaggc gattaatgct gagattcagt cgttgattgc tgcctgccaa
     3661 cgttgccccg caaaggttgt attagtgact aacgaagtgg gaatggggat tgtgccggag
     3721 agtcgtctgg cacgacattt tcgtgatatt gccgggcgga taaatcagca gttggccgct
     3781 gcggcaaatg aagtatggct ggtggtttcg ggtattggag taaaaatcaa atgagtaaat
     3841 tattttgggc gatgctctca tttattacgc gtctgcctgt accgcgtcgc tggtcccagg
     3901 gactggattt cgagcattat tctcgcggta ttattacttt tcctttgatt ggattattgc
     3961 ttggcgcgat tagcgggctg gtcttcatgg tgctgcaggc atggtgtggc gtaccactgg
     4021 cggcactgtt tagcgtactt gtgctggcgc tgatgaccgg gggattccat ctggacggcc
     4081 tcgctgatac ctgtgatggc gtattttctg cacgtagccg cgatcgcatg ttggaaatca
     4141 tgcgtgatag ccgtttaggc acccacggcg gtctggcatt gatttttgtg gtactggcaa
     4201 agattctggt gttaagcgag ttgactctgc gtggcgaacc gatccttgca tcgctggcgg
     4261 cagcatgtgc ggtcagccgc gggactgccg cattattgat gtatcgtcat cgttatgcgc
     4321 gggaagaagg gcttggcaat gtgttcattg gcaaaattga tgggcgacaa acctgcgtca
     4381 cactcggctt agccgctatt tttgctgccg tattattgcc gggtatgcat ggtgtggctg
     4441 ctatggtggt gacgatggtg gcgattttca tcctcggtca gcttctcaaa cgtacgctgg
     4501 gcgggcaaac gggcgatacg ctgggagcag ccatagagct tggtgaactg gtcttcctgc
     4561 tggcactgct ataaagagac gtgacatgca aatacttgcc gatttactga atacgatccc
     4621 agccatcgat cctgccgcta tgtcgcgtgc acaacggcat attgacgggt tactcaaacc
     4681 tgttggtagc ctgggaaagc tggaggcgct tgccatacaa ctggcaggaa tgccggggtt
     4741 gaatggcata ccgcatgtgg gcaaaaaagc ggtactggtt atgtgtgccg atcacggcgt
     4801 atgggaggaa ggggtcgcta tttctccaaa agaagtgaca gtcatacagg ctgaaaatat
     4861 gacccgtgga acaaccggcg tgtgtgtgct ggcagcacaa gcgggcgcta acgtccacgt
     4921 aattgatgtt ggtattgata ctgctgagcc tatccccggg cttatcaaca tgcgtgtcgc
     4981 acgaggtagt ggcaatattg cttcagctcc ggcaatgagt cgccgtcagg ctgaaaagtt
     5041 gcttttggac gtcatatgtt atacgcggga gctggcaaaa aacggtgtca cgctgtttgg
     5101 tgtaggtgaa ctggggatgg caaacacgac accggcagcg gcaatagtca gcacaatcac
     5161 tggctgggat cctgaagaag tggttgggat tggcgcaaac ctgccgacag ataaactggc
     5221 taataaaatt gatgttgtgc gccgggcgat tacgttaaat caaccaaatc ctcaggatgg
     5281 tgttgatgtc ctggcaaaag tgggtggatt tgatttggtc ggaatagctg gagtgatgtt
     5341 aggtgctgct tcctgcggtt tacccgtgtt gctggatgga tttctttctt atgctgctgc
     5401 gctcgcagcc tgccagatgt ctcctgcaat caaaccgtat ctcattcctt ctcacttgtc
     5461 ggcagaaaaa ggcgcgcgta tagcgctgtc gcatttgggg ctggagcctt atctcaatat
     5521 ggatatgcgt ttaggtgagg ggagtggtgc agctctggcg atgcccatta tcgaagctgc
     5581 ttgtgcgata tacaacaaca tgggcgaact tgctgccagt aatattgttc taccggggaa
     5641 tacgacttct gatttgaaca gctaaaaacg aaaagtctga taaaagctat acttatcgga
     5701 tagtattcgc gacttaggaa agggacatga tgcgtcgtgt aaatattctt tgctcatttg
     5761 ctctgctttt tgccagccaa aatagcctgg cggtaactta tccattacct ccagagggtg
     5821 gccgtttagt ggggcagtcg cttactgcaa ctgttcctga tcacaatact cagccgctgg
     5881 agacttttgc cgcacaatac gggcaagggt taagtaatat gctggaagcg aatccgggcg
     5941 ctgatgtttt tttgccgaag tctggctcac aactcaccat tccgcagcaa ctgattttgc
     6001 ccgccactgt ccgtaaaggg attgtggtta acgtcgctga aatgcgtctt tattactatc
     6061 cgccagacag taatacagtg gaagtctttc ctattggtat cggccaggct gggcgagaaa
     6121 ccccgcgtaa ctgggtgact accgttgaac gtaaacaaga agcaccaacc tggacgccaa
     6181 cgccgaacac tcggcgcgaa tatgcgaaac gaggggagag tttgcccgca tttgttcctg
     6241 cggggcccga taaccccatg gggctgtacg cgatttatat tggcaggttg tatgccatcc
     6301 acggtaccaa tgccaatttt ggtattgggc tccgggtaag tcagggctgt attcgtctgc
     6361 gcaatgacga tatcaaatat ctgtttgata atgttcctgt aggcactcgt gtacaaatta
     6421 ttgatcagcc agtgaaatac acaacggaac cggatggttc aaagtggctg gaagttcatg
     6481 agccgctgtc gcgcaatcgt gcagaatatg agtctgaccg aaaagtgcca ttgccggtaa
     6541 ccccatcttt gcgggcgttt atcaacgggc aagaagttga tgtaaatcgc gcaaatgctg
     6601 cgttgcaacg tcgatcggga atgcctgtga atatcaaccg taacggattt tgacattcaa
     6661 aacgtctggt ttgtcggtct gtgtaagcat tccgtcatcc agaacaggtt gtttcgaatc
     6721 atacaaatga taattttctt ctcatatgcg catctcttat gagcataaac gttatactct
     6781 tctgatttat acatgtttgc ggatgacatg atcaatggaa aagagaatga cgaaacagaa
     6841 taaaaacagc cgttggcagg gttacgatcc tcgttacata tactcgctcg tggtcagacg
     6901 ctattttgcg gatatgaaaa ccaagattga gattgctgag gagcttggcg tttcccgctt
     6961 taaagttgcc agattgatcg atgaggcgat cgaacaggat tacgtgaggt ttatcttccc
     7021 taagcagcaa gcgatggatg aagaaatcgc caataatcta cggaaaaaat ttcatctgga
     7081 agatgcaatt gttctttcag ttgctgaatc atggacgacg caagaagaac tcaatcacaa
     7141 attgggtgaa gtcaccgctg aatatcttat gcaatctctt catgaaggta tgaaagtggg
     7201 gatcgcctgg ggacgtgtat tatcaagtac ggtcagtaag ttgagcaagc tgcctccttt
     7261 agacgttgtg cagttatctg gcgtacatcc ggggattgag tttagtcaag ggccaataga
     7321 tcttattcat aagatcgctg ccatttcgca gggaaaagcg cacccaatgt acgtgccgat
     7381 gtgggtcgat gacgaagagc ttgctgccag actggcaggt gatccagcgg tattagatac
     7441 acagcaatat tactcgcagt tggatgtggt tatcaccggg ataggtgact ggaaatcagg
     7501 ttcttcaagc ttgtgtaaaa tattcccgga tacctggtgc gaagctttgt ttcaacaaga
     7561 tatcgctgcg gatgtgtgta tctcgttggt cagcagcgaa gggaagattc ttcatagtcc
     7621 tattgaacgt ctgggatttg gcatttcgac ggatcaacta caaaaagcca aaaaagtgat
     7681 tggtgtcgcc ggaggggaag agaaatatga aggcattctt gcttcgctga aatctggact
     7741 tttaaacgtc ttaattactg attttgatac ggctattaaa cttctggatt aataggcgta
     7801 tcagtgaaga aagggcagta ccgctgattc tgccctttta atttcaggat gctgcgagag
     7861 tatcctctac atatttagct acgcgcgttg caggtaaatt gcggcaagtc tcaacaaagg
     7921 cgttatcctg aaacagtgtg aataaacgac gcaatatcga catatgttga tcactggtgg
     7981 caaatgccag gttaaacata atgctacaat caactgttaa atcagcatct tccatttgcc
     8041 cgaaagtgat atcattggcg aaacgaacaa tactgatact gtttacattt accagcgtgt
     8101 aatcggcatg gggaatggcg ataccctgac cgtttgccat cattagccct gtcggatagc
     8161 ttttttcacg ttccaggcag gcgtcaatgt attctgcagt gatgtagcct ttttccaata
     8221 attgcgtacc agcaaaggtg atcgcagttt gatagtcact gatgctgtta tcaacatcat
     8281 tcaccagaat ttcaaatttc attgcacacc tgttatttca acatctcttc tttaactgca
     8341 gcgaaaat
//
LOCUS       DAZFJL010000171         8286 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFJL010000171 DAZFJL010000000
VERSION     DAZFJL010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.173"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(357..1214)
                     /gene="murI"
                     /locus_tag="ACHJ8C_004184"
     CDS             complement(357..1214)
                     /gene="murI"
                     /locus_tag="ACHJ8C_004184"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO5553953.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEVFIVERVVEIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLD"
     gene            complement(1159..3003)
                     /gene="btuB"
                     /locus_tag="ACHJ8C_004185"
     CDS             complement(1159..3003)
                     /gene="btuB"
                     /locus_tag="ACHJ8C_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO5553954.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDHPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDVWSGFVRGYGYDNRTNYDAYYSPGLPLVDTRKLYSQSWDAGLRY
                     NGKLIKSQLISSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNIGAG
                     VDWQKQSTAPGTAYVEDGYDQRNTGIYLTGLQQVGDFTFEGAGRSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGTYGNPNLNPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(3068..3258)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3372..4472
                     /gene="trmA"
                     /locus_tag="ACHJ8C_004186"
     CDS             3372..4472
                     /gene="trmA"
                     /locus_tag="ACHJ8C_004186"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO5553955.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPILR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQQAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPIAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4512..4871)
                     /gene="yijD"
                     /locus_tag="ACHJ8C_004187"
     CDS             complement(4512..4871)
                     /gene="yijD"
                     /locus_tag="ACHJ8C_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO5553956.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4871..5521)
                     /gene="fabR"
                     /locus_tag="ACHJ8C_004188"
     CDS             complement(4871..5521)
                     /gene="fabR"
                     /locus_tag="ACHJ8C_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO5553957.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTTIIPGNVKDE"
     gene            complement(5625..6941)
                     /locus_tag="ACHJ8C_004189"
     CDS             complement(5625..6941)
                     /locus_tag="ACHJ8C_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HFO5553958.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADHNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPIGCWLR
                     LSLENDVPEPVRNKKAATSESAFSLLLQHKATIVNGVLLAIGSTVATYISLFYYGTWA
                     AKYLAMPQHYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLLCSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            complement(6984..8150)
                     /locus_tag="ACHJ8C_004190"
     CDS             complement(6984..8150)
                     /locus_tag="ACHJ8C_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HFO5553959.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGVVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDRFPCDVIFGM
                     HNMPGLPVGKFFFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
BASE COUNT         2196 a         2230 c         1941 g         1919 t
ORIGIN      
        1 tgaattaaac ttcgtaatga attacgtgtt cactcagaga cttggtattc attttttcgt
       61 cttgcgacgt caagaatccg tatcttcgag tgcccacaca gattgtctga taaattgtta
      121 aagagcagtg ccgcttcgct ttttctcagc ggcgcggggt gtgcatgata cgctttcccg
      181 ctacagagtc aagcatttat ttttgctttc tctgccggaa ttctcaggag aaccccgctg
      241 acccggcggc gtgtttgccg ttgttccgtg tcagtggtgg cgcattatag ggagttattc
      301 cggcctgaca agaggaaatt taaaataatt ttctgaccgc gcaacattca accaaattaa
      361 tctaaaactg ccagtttttc gagcgtttcg aagccgtaac gctgtaaaac gggcaataat
      421 tgttcagctt ctggcgtcat tgccatacaa aatgcaatat tcgcatcggc agattttgca
      481 tccggggctt catgctctaa caaccaggcc gttcggcgag caatcgctgc gccagaatcc
      541 accagccggg ttccctctgg cagcacttgt aatagttctt cttgtagtag agggaaatgg
      601 gtgcaaccca atacaactgt atctggcggc tctttcattc ttaaccacgg gcgtaagata
      661 cgttttagcg catccagaga aacatcttcg ccatgcagct tcgcttcagc caactcaacc
      721 atctctgccg agcccagcat ttctatctgg cattcattag cgaaacgcgc gatcagctca
      781 tgagtataag aacgtttaac agttccgcgg gttgccagta atccgacaat gccatttgcc
      841 gtcagacgtg cagcaggttt aatcgccggc acgacaccaa caaccgggaa gtcgaacttt
      901 tcgcgtaatg caggaagtga aacggtactg gcggtgttgc aagcgaccac agccagcgca
      961 aggggataac gttcttgcac tgcggtgaca atttccacca ctcgctcaac aataaacact
     1021 tcgctttttt cgccatacgg gaaagcgacg ttatcgaaag cataaatgta atggagatcc
     1081 ggtaagagat gccggatctc gtcatagacc gacaacccac cgacgccgga gtcaaacacc
     1141 agcacggtgg gacgtggttc agaaggtgta gctgccagac aaggtgtatt cccgtcctgc
     1201 agtttggtag ccatagactg tctcataatc tttgtcgaac aggttggcta ttttaccacg
     1261 aactgtcagg tgagaggtga ccggatacgc aaccgcaaga tcccacaagc tcacaccgcc
     1321 cattttaacg gtttgataag gataagatga gtaatcctta tcatagcgag tgcctaaata
     1381 ctgataagta atcccccagt cgaagtcata caactgccag tcgagctggt atttcacctg
     1441 ctgtttagca cggcgtaaca acggcgtgtc agtaattgca ttgcgcgcat caacataatc
     1501 ataactcaca gtatgcgtca gtggtccggt atcaaaatta gcggtcgcct cgacaccctt
     1561 aatccgcgct ttcccttcgt tgtaatattt cagggtgtga tcatcataat cgatcaagtc
     1621 actgacatcg ttacgatatc cggaaatacg ccagttaacc ccagcagtta agccttcaaa
     1681 cgcgccttcc cattgtttgc ttttctcagg attcagattc ggattaccgt aggtgccata
     1741 cagttgccca agatttggtg ccttataaga tgttccgtag gaagcaataa agcgataacc
     1801 ttcgatgaac tcccaaccgg cgctggtttg ccaggtacca tgacgaccaa actgagaatt
     1861 atcgtcgctg cgtcctgcgc cttcaaaagt aaaatcgccg acttgttgca gcccagtcag
     1921 atagatgccg gtattacgtt gatcatatcc atcctcaaca taagctgtgc ccggtgccgt
     1981 gctctgcttc tgccagtcaa cacccgcacc aatattaccg tggccaatga tgatgttgtt
     2041 tgcccactgg acggtgtact gtttcatctc atcgagcgtc gcggacgaat cataacgacc
     2101 ataatgcgga tcgtagttgt aatctttgct atggctgtag ctggaaatca gttgtgattt
     2161 aatcagtttg ccgttatagc gcagtccggc atcccaactt tggctataga gtttacgggt
     2221 atcgaccagt ggcaagccgg gagaataata tgcgtcataa ttggtacgat tatcatagcc
     2281 atagccgcgc acaaagccgc tccagacatc agtaaaatta tgctccagcg cgccataaag
     2341 cgttttactt aaaaaaccat cgttatctgg ctgcgcttgc gttccggtat taccataggc
     2401 gacaacatca taaccatgag tatgggcata atcgcccaac agcgttaccc gtgtcttatc
     2461 ccccagttgt tgctgtgtag agacatcata gttctgataa ctattgcttc cccaccctgc
     2521 tgaaatttcc gttccggggt gatcgcgcgt cgtgatgata ttcacgaccc cgcctattgc
     2581 atctgaacca taaacagcgg agcgcggccc acggatatat tcaacacgct ggacaagcgc
     2641 aatagggaac tgactaagat cagcagaacc actcaccccc gccaaattca ggcggacgcc
     2701 atcaattaac accaacacat gactggcatt tgtaccgcga ataaaaatag atgagagctg
     2761 acctgaaccg ccgttttggg tgatatccac gcccggaaga cggcgcagca catcattgac
     2821 cgaggtcgac tgccagcggt cgatatcctg acgcgtcaca acagtagttg gcgcaagcac
     2881 agtgctgcgc ggttgttcaa aacggttagc agtaacgacg agagtatccg ggctggtatc
     2941 ctgtgcccaa gcggaaaatg ccgtgacgga acacgccgtc agcagcgaag cttttttaat
     3001 cattgtaaag catccacaat agaagaagga tgccgcaggt ttcatcaata ttacgcgatg
     3061 atgagaacca gatgcgacgt tggccggcag gtcttcgggc ttggaggggt atctaagata
     3121 ctaagagatg atgacttccc accggatggc agtgtccgca taacgcaatc atcgcacctt
     3181 tccttaccgc tgcgcgtcag ctccagattc gcactggatt ccctattaac tcacaggacc
     3241 ggcaagtgga tgctacaggt tgtaacaagt tactgtccag acgtagctca caaataggaa
     3301 ttcatcaaga tctggacatc tgatgagcaa tccctacaat cgccgcgtac tttaattttt
     3361 caggatacat catgaccccc gaacaccttc caacagaaca gtatgaagcg cagttagccg
     3421 aaaaagtggt acgtttgcaa agtatgatgg caccgttttc tgacctggtt ccggaagtgt
     3481 ttcgctcgcc ggtcagtcat tatcggatgc gtgcggagtt ccgcatctgg cacgatggcg
     3541 atgacctgta ccacatcatt ttcgatcaac aaaccaaaag tcgtatccgt gtggatagct
     3601 tccccgccgc cagtgaactt atcaaccagt tgatgacggc gatgattgcg ggtgtgcgta
     3661 ataatcccat tctgcgccac aagttgttcc agattgatta cctcactacg ctaagcaatc
     3721 aagcggttgt ttccttgctg taccataaaa agctggatga tgagtggcgt cagcaggcgg
     3781 aagccctgcg cgatgcactg cgcgcgcaga atctgaatgt gcatctgatt ggtcgggcaa
     3841 cgaaaaccaa aatcgcgctg gatcaggatt acatcgacga acgtctgccg atcgcgggga
     3901 aagagatgat ctaccgtcag gtagaaaaca gctttaccca accgaacgcg gcgatgaata
     3961 ttcagatgct ggaatgggcg ctggacgtaa ccaaaggctc aaaaggcgat ttactggagc
     4021 tatactgcgg caacggtaac ttttcattag cgctggcacg caattttgat cgggtattag
     4081 ccaccgaaat cgccaagccg tcagtcgccg ccgcgcaata caacattgcc gctaaccata
     4141 ttgataacgt ccaaattatt cgtatggcgg cagaagaatt tactcaggcg atgaatggcg
     4201 tacgcgagtt taaccgcctg caagggatcg acttaaagag ctatcagtgc gaaaccattt
     4261 ttgtcgaccc gccgcgcagc ggtctggaca gtgaaaccga gaaaatggtg caggcgtatc
     4321 cacgtattct gtatatctcc tgcaatccgg aaacgttatg caagaatctg gaaacattaa
     4381 gtcagacgca caaggtcgaa cgtctggctc tgtttgatca gttcccctac acgcaccata
     4441 tggagtgcgg cgtattactg accgcgaagt aaaacccatg ccggatgcat cagcatccgg
     4501 catgatttcg attactcagc aacttcctgc ttacggttac gcatcttcgc gccaatccag
     4561 aacaccataa tgacggacag tactgccggg aagaagttag agccgatatc cggatactcc
     4621 gcacgaacta ccgtgctgta cagcagtacg ccgagaataa aacaggcagc tgccagaccc
     4681 ggcaagccta ccggcatggt gcgattaaga taacgttgat gcaggcagta aaccgtcagc
     4741 accagggaaa taatcgggaa tacagaaaat ggcacaatgg agctaaacag cgctgcgaaa
     4801 gtaccattaa tcgataagcc agcaaccaac gccagcagca gcgtacctct atcttgattt
     4861 gcttgtttca ttactcgtcc ttcacatttc ccggaataat tgtggttttc tcttgttcac
     4921 ggcgatacca gtaataagcc cctttcgaaa tcattcgcag ctgcagtacc agtcgctctt
     4981 ctaattgccg gcgttgctcg acgccgacat ccaacgcctc ggcccccgca ctgaagacaa
     5041 tcgtcaccat tgcttcggct tgcgcttcag taaacgcacg cggcatatgg ttttcgagtt
     5101 ccagatagtc cgcaagttcc gcaatgaagt gctgaatttc acgcgcaacg gcggcacgaa
     5161 acgcagcgga ggtgccggag cgttcccgca ataataaccg gaaggcgtta ggattattac
     5221 cgatgaactc cataaatgtg gagaccgagg tgcggatcac actcccgcct ttggcgatac
     5281 gctgacgcgc ctgacgcatg agttggcgta gcattaaacc gctctcatca accatggtca
     5341 gacctagttc gtctacgtcg cggaaatgcc gataaaaaga ggtgggagca atgcctgctt
     5401 ctcgtgccac ttcacgcaaa ctcaggctgg cgaagctgcg ttcagcactt aattggctaa
     5461 atgcggcttc caccagcgaa cggcgggttt tttctttttg ttgcgctctt acgcccatca
     5521 cgatgtctga atccttgcca aaagtactgc tggcactata ccagagaatg aacatactgg
     5581 attgctccag tgttgtgaat gaacggtaac gcaataaaac atatttactg caattctttg
     5641 gccggttctt ttacgtacaa caacgccagc atcgacagca acgtgcctcc ccccagatac
     5701 cacgccggag ccgtcagact atccagcaac acgatggatt gcgtggcgaa atattgcgca
     5761 aaaccgccaa agattgctac gccaatgcta taaaccagcg cgaaacctaa tgcccgaatc
     5821 cgtttcggca acagttcaga gatcaacaac atcactggca caccgccaag cgtggtaaag
     5881 ctgaccatca cgaaaaccac cgtcagcaac atgccagggc ttgggtaatt caccaacagc
     5941 cagaatgacg gccagctaca gagaagcacc atcacacggg agattaaaat cagcttttta
     6001 cgcccaaccg aatcacatag catccccacc aacagcgcac ccacaaacgt aattaccccc
     6061 gccagtaaca tcgctgcatg agaataatgt tgaggcatcg ccagatactt cgccgcccag
     6121 gtgccgtaat aaaagaggga gatataggtt gcgaccgtgc taccaatagc gagtaaaacg
     6181 ccattaacga tagtcgcctt atgttgcagg agcagagaaa acgcgctttc gctggtagca
     6241 gcttttttat tgcgcactgg ctcaggtacg tcattttcca ggctcagacg caaccagcaa
     6301 ccgataggtg ctaataacac gccaataaaa aacggcacac gccagcccca ttctgccatt
     6361 acggtttctg aacctgtggc gaacggtagc catgcgctca gacctaaagc aacaacccca
     6421 ccgaaggtcg ttgccagccc ttgggtcgcc agtgaccaac tgctgtaaaa gccgcgacga
     6481 tttgccggag ctgactccac cagtagcgac attgaagccc cgacttcacc acctgccgca
     6541 acaccctgaa ttagacgcgc cagcaccagc gttgcagttc cccaatagcc cgccgtttca
     6601 tacgtcggtg cgatgccgat catcagcgta ccgaggctca tcagagcaat agtgaacacc
     6661 atcgccggtt tacgcccgaa gcgatcggcc caggcaccaa cgataatccc accgagcgga
     6721 cgcataaaaa agctcacacc aaacgttgca aatgccagta acagcgcgac gttatgatcg
     6781 gcactgggtg ggaagaaaag cgtactgata tagaccacaa aaaagttgta aacgccaaaa
     6841 tcaaaaaatt cgagcgcatt gccaaacgcc gaggcaaaga tcacccgcct gccgctgggc
     6901 tttttcttca cactcacggt ttcatttacg atcgccgtca ttccatgttc cttgccaaaa
     6961 agtgttgttg tgtgttgttt tatttattgc agccaggctt caaccagtgc gccccagtag
     7021 ctgcttgccg ggaccaggct tgcatcgtta aagtcgtaac cggggttatg caccatgcag
     7081 gaaccttccc cgacatcgcc attaccgaat aacaggaaac cgcctttggg ttgggcttcc
     7141 agcatgaaag agaaatcttc actgcccatc aggggtttga tgccaaactc agcccgatct
     7201 tcaccaaatg ttttgcacgc cacctgccag gcgaagcgcg ccatctcctc gtcatttact
     7261 aataccggtg taccgttaac atgcgtaacc tcagccgtag cgccaaagct ggcagcctgg
     7321 gcttgcgcca gagcaggaat acgggtcaga agaagttgtc gggtatcgcg gctaagagag
     7381 cggacactga ttttcatctc agcactgtca ggaatgacgt tagccgcctc acccgcaaca
     7441 atgctgccga cggtaattac cgccgcttcc agcggatcga cattgcgtga cacaatgctt
     7501 tgtaatgcgg tggtgatatg tgcggcaacc agcaccggat cgatggcttt gtgcgggatc
     7561 gcaccgtgtc cgccacaacc acgaaccgta atatggaact gatccattga cgccatcagc
     7621 gccccaggct ggaagaaaaa cttgccgacc ggcaaacccg gcatgttgtg catcccgaaa
     7681 atgacatcgc aggggaaacg atcaaaaagc ccctctttaa ccatgatctc accgccgtta
     7741 atcatctctt ccgcaggctg gaagataagg cgtaacgtgc cgttaaaacg gcgggtttcg
     7801 gcaaaataac gcgcagcccc cagcagtatg gtggtatgcc cgtcatgccc gcaggcgtgc
     7861 attaaaccag gatgtttact ggcccagggc ttgccactat tttcataaat cggcaacgca
     7921 tccatatccg cccgcaggcc aatacttttc tctccgtcgc ccacttttaa cgtggctacc
     7981 acgccggttg tcgccagccc ttgctcaacc tcatagcccc actggcgcag cttttccgca
     8041 accaaagcac tggttttaaa ttcttgtaat cccagctccg ggttttcgtg gatctgatgg
     8101 cggatctcgc gaagttcttc ctcaaaccca cgaatatagt gttcgatcat cggctattcc
     8161 tttattcact tataaacagg ctaattaacg gattttattg atggtataaa tacaaataac
     8221 tcatcaatat aaaatattta tatatttgtt ttataagaag atttcataaa gacaataaca
     8281 aaaaag
//
LOCUS       DAZFJL010000172         8052 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFJL010000172 DAZFJL010000000
VERSION     DAZFJL010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.174"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(171..2492)
                     /locus_tag="ACHJ8C_004191"
     CDS             complement(171..2492)
                     /locus_tag="ACHJ8C_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO5553960.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGILQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(2589..3065)
                     /gene="greB"
                     /locus_tag="ACHJ8C_004192"
     CDS             complement(2589..3065)
                     /gene="greB"
                     /locus_tag="ACHJ8C_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO5553961.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            3293..4012
                     /gene="ompR"
                     /locus_tag="ACHJ8C_004193"
     CDS             3293..4012
                     /gene="ompR"
                     /locus_tag="ACHJ8C_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO5553962.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            4009..5361
                     /gene="envZ"
                     /locus_tag="ACHJ8C_004194"
     CDS             4009..5361
                     /gene="envZ"
                     /locus_tag="ACHJ8C_004194"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO5553963.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGMTKEG
                     "
     gene            complement(5392..5688)
                     /gene="nadS"
                     /locus_tag="ACHJ8C_004195"
     CDS             complement(5392..5688)
                     /gene="nadS"
                     /locus_tag="ACHJ8C_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO5553964.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEETPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSWETKRRQPTGAPRKLLLLLQANPFIINQLKTI"
     gene            complement(5747..6064)
                     /locus_tag="ACHJ8C_004196"
                     /pseudo
     CDS             complement(5747..6064)
                     /locus_tag="ACHJ8C_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493756.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6174..7796)
                     /gene="pckA"
                     /locus_tag="ACHJ8C_004197"
     CDS             complement(6174..7796)
                     /gene="pckA"
                     /locus_tag="ACHJ8C_004197"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO5553965.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGSIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
BASE COUNT         1786 a         2062 c         2164 g         2040 t
ORIGIN      
        1 aggtttcagt tgttaatgaa gtgataaata ttaacgggat atattatgag tttgcttgtg
       61 gaataaaaag gccggagata ttctccggca cttttatgtc aggcaaggcg ttcgttgcca
      121 gatgtggcgt gaacgcctaa ttgcgcaggt ttaggggcca gtccgggcgt ttaacgtttt
      181 ttgcccattg ccgccgccag cgcatccatc atcgcgctat taccggcagg ctgcgcttca
      241 cgcccacgcg gtttggctgc cgggcggttg ttttgcgggc gttcattacc gccgccgcga
      301 cgggcgttgg tttcgccagg ctgctcgtcc agacgcatgg tcagggcgat acgtttacgc
      361 tgaagatcca cttccagcac tttcaccttc acaatgtcgc ccgctttcac cacggtatgc
      421 ggatcttcca caaacttgtt cgacaatgaa gagatgtgaa ccaggccgtc ctgatgcacg
      481 ccaatatcga caaacgcgcc aaagttggtg acgttggtca ccgcaccttc gaggatcata
      541 cccggttgca ggtcgttcat tgtctcgacg ccatcggcaa actgagcggt tttaaattcc
      601 ggacgcggat cgcgacccgg tttttccagc tctttgatga tgtcggttac cgtcggcaca
      661 ccgaatttct catcagtaaa gtcagacgct ttcaggttac gcagttcgct gctgttaccc
      721 atcagatctt tcagtgcctg ctgtgttgct gccagaatgc gttccaccac tggataggct
      781 tccgggtgaa cggtagaagc gtccagcggg ttatcaccgt ggttaatgcg caagaagccc
      841 gcgcactgct cgaaggcttt cggccccaga cggctcactt ttaacagttg ctgacggttc
      901 tggaactggc cgttctcatc gcgccaggcc acgatgtttt gcgccatcat gcgcgtcagg
      961 cccgccacgc gggttaacag aggaacagaa gcggtgttga gatcgacgcc aacggcgttt
     1021 acgcagtctt ctactactgc atccagtttg cgtgccagct gcgtctggct gacgtcatgc
     1081 tgatactgac ctacgccgat agatttcgga tcgattttca ccagctccgc cagcggatcc
     1141 tgcaaacgac gggcgataga caccgcgcca cgcagcgaaa cgtcgagatc cgggaattcc
     1201 tgtgctgcca gctcggaagc cgagtaaacc gacgcgccag cttcgctgac gatcactttc
     1261 tgcgcggtca ctttcgggaa ctgcttctgc acgtcgagat agaaacgttc ggtttcgcga
     1321 gaagctgtac cgttgccgat cgccaccagt tcaacgttat gcttttcaca cagcgcagca
     1381 acggtcatcg ctgcttttgc ggcctgcccg gtatgcggat aaatggtatc ggtcgccacc
     1441 agtttgccag ttgcatcgac taccgccact tttaccccgg tacgcagacc cggatcgagg
     1501 cccatcgttg cacgcagtcc ggcaggggcc gccatcagca gatcgtgcag gttacgggca
     1561 aagacgttga ttgcttcatc ttccgcgcgt tcgcgcacgg tgcccatcag ttcagtttcc
     1621 agatgcatca acaccttgat gcgccacgtc cagctcacca cacctttgcg ccagctatcc
     1681 gccggggcat tgttcaggcg caggccaagg tgatccatga tgatttgctc gcaatagctc
     1741 tctttcggcg gctcttcgaa ctgcggatcg gcattcagcg aaagctgaag tatgccttcg
     1801 ttacgaccac ggaacatcgc cagtgcgcgg tgagaaggca ccgttgacag tggttcgtga
     1861 tgatcgaaat agtcgcggaa ttttgcccct tcctcttctt taccgctcac cacggttgag
     1921 accagatgcg cgttcttcca cagataatca cgcactttcg ccagcagcgc agcatcttcg
     1981 gcaaaccgtt ccatcaggat atagcgcgcg ccgtccagcg cggctttggt atctgccacg
     2041 cctttatcgg catcaatata ttgtgcagcg gcgacttctg gcgtgtgtga cggatcgctc
     2101 cacagcagat cggccaacgg ctcaagccct gcttcaatgg cgatttgccc acgggtgcgg
     2161 cgtttaggtt tgtagggcag gtagaggtct tcgagttcgg ttttgcttag ggtggcgttg
     2221 atggcattcg ccagatcatc ggtgagtttg ccttgctcgg aaatagactt gaggatcgcc
     2281 tgacgtctct cttccagctc gcgcagatag ctcagacgcg tttccagatt acgcagctgc
     2341 gtgtcatcca gaccgccggt gatttcctta cgataacgtg cgataaacgg cacggtattc
     2401 ccttcgtcaa gcaggcgaac ggcagcgtca acctgttccg ggcgcgcctg aatttcaccc
     2461 gcaataatgc ggcagaacga atcattcatc atggtttgag ttcatctttt cggatcaaaa
     2521 atcaggggat agttatacgg actggctggc aaaaatgcca gccatcggca ggaggttaag
     2581 actcttcctt acggtttcac gtactcgata gcattaacat accagctcgc ttccccggca
     2641 ggggtattca ccaccgccag atcgccgact tcttttttca gcaatgcgcg ggccatcggg
     2701 gaatcgatag agatgtaatc tttacggcca aaaatttcat cgtagccgac aatacggaaa
     2761 cggtgagtca cgccatcgtc gttttcaatc tccacccacg cgccaaaaaa gactttgcct
     2821 tcctgctgcg gggagtaatc gacgattttg agattttcca tgcatttagt gagatagcgc
     2881 acgcgacggt cgatttcacg caggcgcttt ttattatact gatagtcagc attttcgctg
     2941 cggtcgccca gacttgcggc ccaggtcact ttctttgtga cctccgggcg ttcttcgcgc
     3001 cagagataat taagctcttg tttgagtttt tcataccctt cccgggtaac caggggcgtt
     3061 ttcatctcgt tgattccctt tgtctgtttg ataatgcgca cattgggtat aacgtgatca
     3121 tatcaacaga atcaataatg tttcgccgaa taaattgtat acttaagctg ctgtttaata
     3181 tgctttgtaa caatttaggc tgaaattcat accagattta gctggtgacg aacgtgagct
     3241 tttttaagaa tacacgctta caaattgttg cgaacctttg ggagtacaaa caatgcaaga
     3301 gaactacaag attctggtgg ttgatgacga catgcgcctg cgcgcgctgc tggaacgtta
     3361 tctcaccgaa caaggcttcc aggttcgaag cgtcgctaat gcagaacaga tggatcgcct
     3421 gctgactcgt gaatctttcc atcttatggt actggatttg atgttacctg gtgaagatgg
     3481 cttgtcgatt tgccgacgtc ttcgtagcca gagcaatccg atgccgatca ttatggtgac
     3541 ggcgaaaggg gaagaagtgg accgtatcgt tggcctggag attggcgctg acgattacat
     3601 tccaaaaccg tttaacccgc gtgaattgct ggcccgcatt cgtgcggtgc tgcgtcgtca
     3661 ggcgaacgaa ctgccaggcg caccgtcaca ggaagaggcg gtaattgctt tcggtaagtt
     3721 caaacttaac ctcggtacgc gcgaaatgtt ccgcgaagac gagccgatgc cgctcaccag
     3781 tggtgagttt gcggtactga aggcactggt cagccatccg cgtgagccgc tctcccgcga
     3841 taagctgatg aaccttgccc gtggtcgtga atattccgca atggaacgct ccatcgacgt
     3901 gcagatttcg cgtctgcgcc gtatggtgga agaagatccg gcgcatccgc gttacattca
     3961 gaccgtctgg ggtctgggct atgtctttgt accggacggt tctaaagcat gaggcgattg
     4021 cgcttctcgc cacgaagttc atttgcccgt acgttattgc tcatcgtcac cttgctgttc
     4081 gtcagcctgg tgacgactta tctggtggtg ctgaacttcg cgattttgcc gagcctccag
     4141 cagtttaata aagtcctcgc gtacgaagtg cgtatgttga tgaccgacaa actgcaactg
     4201 gaggacggca cgcagttggt tgtgcctccc gctttccgtc gggagatcta ccgtgagctg
     4261 gggatctctc tctattccaa cgaagctgcc gaagaggcag gtctgcgttg ggcgcaacac
     4321 tatgaattct taagccatca gatggcgcag caactgggcg gtccgacgga agttcgcgtc
     4381 gaggtcaaca aaagttcgcc tgtcgtctgg ctgaaaacct ggttgtcgcc caatatctgg
     4441 gtacgcgtgc cgctaaccga aattcatcag ggcgatttct ccccgctgtt ccgttatacg
     4501 ttggcgatta tgctattggc gataggcggg gcgtggctgt ttattcgtat ccagaaccga
     4561 ccgttggtcg atctcgaaca cgcagccttg caggtgggta aagggattat tccgccgccg
     4621 ctgcgtgaat atggcgcttc ggaggtgcgt tccgttaccc gcgcctttaa ccatatggcg
     4681 gctggtgtta agcaactggc ggatgaccgc acgctgctga tggcgggggt aagccatgat
     4741 ctgcgcacgc cgctgacgcg tattcgtctg gcgaccgaga tgatgagcga gcaggatggc
     4801 tacctggcag aatcgatcaa taaagatatc gaagagtgca acgccatcat tgagcagttt
     4861 atcgactacc tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct caacgcagta
     4921 ctcggtgagg ttattgctgc cgaaagtggc tatgagcggg aaattgaaac tgcgctttac
     4981 cccggtagca ttgaggtgaa aatgcacccg ctgtcgatca aacgcgcggt agcgaatatg
     5041 gtggtgaacg ccgcccgcta tggcaatggc tggatcaaag tcagcagcgg cacggagccg
     5101 aatcgcgcct ggtttcaggt agaagatgac ggtccgggta ttgcgccaga acagcgtaag
     5161 cacctgttcc agccgtttgt tcgtggcgac agtgcgcgca ctattagcgg cacgggatta
     5221 gggctggcga ttgtgcagcg tatcgtggat aaccataacg ggatgctgga gcttggcacc
     5281 agcgaacggg gcgggctttc cattcgcgcc tggctgccag tgccggtaac gcgggcgcag
     5341 ggcatgacaa aagaagggta aaaaaacggg aggtgaaggt gcctcccgtt tttagattgt
     5401 tttcaattga ttgataatga aaggattcgc ttgcaaaagc aggagcaatt ttcggggtgc
     5461 tccggtaggc tgacggcgtt tggtttccca gcttttgacc aggtcataac ttacccctac
     5521 ggcgacagca aaatcttgct gacgtaaacc cgttgcttca cggatcgctt ttacatcgat
     5581 ttcgctaaaa gtatggacgt gctctggttc aggcgtttct tcaccctttt cgatgcgtac
     5641 catttcttct gcgctggcaa gaagatcagc aaataattca tctttcatct gttttcaatg
     5701 tctccgttgg catttcgacc atggtggaat ttgtggagct tcaacattaa agcagcttct
     5761 cggacaatag tttcaatgcc tttctttgtt cttctgttaa atcatcctgt tcatttttag
     5821 gataaatcaa cgccagataa attcgaccat tgcgtgttac gttgtaataa attattcgaa
     5881 tgccaccccg tttccccatt ccagaacggt tccagcgaat ttttctaaaa ctgcctgtgc
     5941 ctgcgataat gtcaccggca gtgggatctt gaatgagtag ttcctgaaaa gcccggaact
     6001 acttatcagg tagcaataac tggcggcgtt tgctaaatcc ttgtagttca ataaaggtga
     6061 acataatgca tcctgcggtt tattgataat tataagtgta catattggta gctaattaga
     6121 tataaaaaaa ccccttatcc gtttggataa ggggtcaatg agatatcagc tgattacagc
     6181 ttcggaccag ccgctaccag cgcggcaccc gcaggggtgt cggtgtattt atcgaagttg
     6241 tcgataaaca gttttgccag cgtttcggct ttttcctgcc actgttccgg agaagcgtag
     6301 gtgttacgcg gatcgaggat cttcgtgtct acgcccggca gttcggttgg gatcgccagg
     6361 ttaaacatcg gcagagtgaa ggtttctgcg ttatccagcg aaccgttgag gatggcgtcg
     6421 ataatagcgc gggtatcttt aatggagata cgtttgccag tgccgttcca gccggtgtta
     6481 accagatacg cctgcgcacc cgccgcctgc atacgtttca ccagcacttc tgcgtattgg
     6541 gtcgggtgca gcgacaggaa tgccgcgccg aagcaagcgg agaaggttgg cgtcggttcg
     6601 gtgatgccac gctcagtacc ggccagtttg gcggtgaagc cagagaggaa gtgatactgg
     6661 gtttgatcgg cagtcaggcg agaaaccggc ggcaacacgc cgaaagcatc agcagtcagg
     6721 aagataacct tagtcgcgtg gcccgctttg gaaaccggct taacaatgtt atcgatgtga
     6781 tagatcggat aagaaactcg ggtgttctcg gtttttgaac catcatcaaa gtcgatagaa
     6841 ccatcttcac gcacggtgac gttttccagc agcgcatcgc gacggatagc gttgtagatt
     6901 tcaggttccg cttctttcga cagcttgata gtttttgcgt agcagccgcc ttcaaagtta
     6961 aacacaccgt catcgtccca gccgtgttcg tcatcgccaa tcaggcgacg tttcgggtcg
     7021 gtggaaaggg tggttttacc ggtgccggaa aggccgaaga acaccgcaac atcgcctttc
     7081 tcgccaacgt tggcggagca gtgcatagaa gcgatacctt tcagcggcag caggtagttc
     7141 atcattgaga acatcccttt cttcatttcg ccgccgtacc aggtgccgcc aatcagctgc
     7201 atgcgctcgg tcaggttaaa cgccacgaag ttttcggagt tcagaccctg ttctttccac
     7261 tgcgggttag tgcacttcgc gccgttcata acgataaagt ctggtttgaa acctgccagc
     7321 tcttcatcgc tcgggcgaat aaacatgttt ttgacaaaat gcgcctgcca ggccacttcg
     7381 gtgatgaaac ggacggaaag acgagtatcc gggttcgcac cacagaaggc gtcgacaacg
     7441 aacagacgtt tgccggaaag ctgcctggtc accaggcctt tcagatgctg ccaggtttcc
     7501 ggagagagag gtttgttgtc gttcttacct ttgcctttgt ctgcccacca gaaagtatcg
     7561 cgagtggtat cgtcacgaac gatatactta tctttcggtg aacgaccggt gaagatcccg
     7621 gtatcgacgg caacggcacc cagattagtt aacaccccgc gctcataacc ggtcaggctc
     7681 gggtccagct cttcctgata cagcagatcg tagcttgggt tgtaaacgat atcatgtacg
     7741 tcactgatac cataagcctc gagttcttgc ggggtcaaac cattgttaac gcgcatttca
     7801 ctgctcctta gccaatatgt attgcctgaa tagtaaagtc tttttggggg tgttaaccgc
     7861 gacaaggctc atagatttac gtatctggag aaattcatat ttgacaaaaa actccgtgat
     7921 tcctgtcacg aaacggttgc tattatcgca gaaatagcat tttaggtgtg aaaaatgttc
     7981 tcaaaaggtt aaatcttggt ttatatggcg ggaatattgt gagtggaatc gcattcatga
     8041 atgaaattga tg
//
LOCUS       DAZFJL010000173         7987 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFJL010000173 DAZFJL010000000
VERSION     DAZFJL010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.175"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            349..891
                     /gene="ecpR"
                     /locus_tag="ACHJ8C_004198"
                     /gene_synonym="matA"
     CDS             349..891
                     /gene="ecpR"
                     /locus_tag="ACHJ8C_004198"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO5553966.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            965..1552
                     /gene="ecpA"
                     /locus_tag="ACHJ8C_004199"
                     /gene_synonym="matB"
     CDS             965..1552
                     /gene="ecpA"
                     /locus_tag="ACHJ8C_004199"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO5553967.1"
                     /translation="MKKKVLAIALVTVFTGTGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1609..2277
                     /gene="ecpB"
                     /locus_tag="ACHJ8C_004200"
                     /gene_synonym="yagY"
     CDS             1609..2277
                     /gene="ecpB"
                     /locus_tag="ACHJ8C_004200"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO5553968.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2303..4828
                     /gene="ecpC"
                     /locus_tag="ACHJ8C_004201"
                     /gene_synonym="yagX"
     CDS             2303..4828
                     /gene="ecpC"
                     /locus_tag="ACHJ8C_004201"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO5553969.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMTLANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTFSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSGTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVNKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGKKNES"
     gene            4818..6461
                     /gene="ecpD"
                     /locus_tag="ACHJ8C_004202"
     CDS             4818..6461
                     /gene="ecpD"
                     /locus_tag="ACHJ8C_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO5553970.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPSGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6430..7140
                     /gene="ecpE"
                     /locus_tag="ACHJ8C_004203"
     CDS             6430..7140
                     /gene="ecpE"
                     /locus_tag="ACHJ8C_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO5553971.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7447..7776
                     /gene="ykgJ"
                     /locus_tag="ACHJ8C_004204"
     CDS             7447..7776
                     /gene="ykgJ"
                     /locus_tag="ACHJ8C_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO5553972.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGQNACCTIYENRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(7788..7960)
                     /locus_tag="ACHJ8C_004205"
                     /pseudo
     CDS             complement(7788..7960)
                     /locus_tag="ACHJ8C_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT         2108 a         2032 c         2019 g         1828 t
ORIGIN      
        1 atataaaatg caagtaagaa ttgaacgtta tattgccaat aacattatga aactgaatgt
       61 ctttttcttc ttatcaaaaa agcaatattt tcattttttg taaatattga cttaaccatg
      121 gaattcattt tctgttcaca tattgacact catcaggaaa aaaacataaa tttaaaccta
      181 atcgaaataa ttaaaactta atctcgttta acctatattg atatgtgcta cgtatcttat
      241 ttacttccga tttactaaag aaactgaatg tacctgtaaa aattacaggt ttggaaagta
      301 gtgacatggc aaaatgatta cagcagggac tatgaggtta aaaaccatat ggaatgtcaa
      361 aaccgttctg ataaatacat ctggtctccc catgacgcct acttctataa aggactatct
      421 gaactgattg tggatatcga cagattaatt tatctatcgc tggagaaaat cagaaaagat
      481 ttcgtgttta tcaatctcaa tacggattct ttaactgagt ttataaaccg tgataatgag
      541 tggttatccg cggtaaaggg gaaacaggtc gtattgattg cggccagaaa gtcagaagcc
      601 ttagcaaatt attggtatta caacagcaat attaggggcg tggtatacgc tggactgagt
      661 cgtgatatta gaaaagaact ggcctatgtg attaatggca ggttcctgag aaaagatatt
      721 aagaaagata aaatcactga ccgggaaatg gaaattatcc gcatgacggc tcagggaatg
      781 ctgcctaaat cgattgccag aattgaaaat tgtagtgtga agacagtgta tacccatcgg
      841 cggaatgcag aggccaagct gtactcaaaa ttatataagt tggttcagta aactccaggc
      901 aagttagttt taaaaatgac tcaccgggac ttcatgtcct caattcaact cgggaagaaa
      961 agcaatgaaa aaaaaggttc tggcaatagc tctggtaacg gtgtttaccg gtacaggtgt
     1021 agcgcaggct gctgacgtaa cagctcaggc tgtagcgacc tggtcagcaa cagccaaaaa
     1081 agacaccacc agtaagctgg ttgtgacgcc actcggtagc ctggcgttcc agtatgccga
     1141 aggcattaaa ggttttaact cacagaaagg tctatttgac gtggctatcg agggtgactc
     1201 aacggctacc gcctttaaac tgacctcacg tcttatcacc aacaccttaa cccagttgga
     1261 tacctcagga tccacactga atgtgggcgt ggattataac ggcgcggcag tcgaaaaaac
     1321 tggcgatacc gtgatgatcg ataccgccaa cggcgtactg ggcggcaacc ttagcccact
     1381 ggctaacggt tacaatgcca gcaatcgtac caccgcacag gatggtttca ctttctccat
     1441 catcagcggc accaccaatg gtaccaccgc agtaaccgat tacagcactc taccggaagg
     1501 catctggagc ggcgacgtta gcgtacagtt cgacgctacc tggaccagtt aatctctctg
     1561 atgtaccagc aggggtaccc ccctgcttta ttcctggacg gaattgttat gaaaaagcac
     1621 cttctgcctc tcgctctgct gttttccgga atatcaccgg cccaggcgct ggatgtcggc
     1681 gatatatcat cgtttatgaa cagtgacagc agcacgctaa gcaaaacgat ccaaaacagt
     1741 accgacagtg gccgcctcat caatatccgt ctcgaacggc tctcttcacc gcttgacgac
     1801 gggcaggtta tcgcaatgga caagccggat gagttgctac tcactcccgc cagcttgctg
     1861 ctacccgccc aagccagcga agtgatccgc ttcttctata agggaccagc agatgaaaaa
     1921 gagcgctact accgcattgt ctggtttgat caggccctca gtgatgcgca gcgcgataat
     1981 gccaaccgta gcgctgtggc cactgcttcc gcccgcatcg gcaccattct ggtcgtcgca
     2041 ccccgccagg caaactacca ctttcagtac gccaacggct ccctgacaaa tacaggaaat
     2101 gcgacgctgc ggatcctcgc ctacggcccc tgcctgaaag ccgccaatgg taaggagtgt
     2161 aaagagaatt actacctgat gccgggcaag tcgcgtcgtt ttacccgcgt ggacacggcg
     2221 gataacaaag gacgggttgc actttggcag ggtgataagt tcattcccgt gaaatagata
     2281 gctgtgcaga tggataacga caatgccttt acgacggttc tccccaggac tgaaagccca
     2341 gtttgccttc ggcatggtct ttttgttcgt tcagcccgat gccagcgctg ctgacataag
     2401 tgcgcagcaa ataggtgggg tgattattcc gcaggccttc agtcaggcgc ttcaggacgg
     2461 catgagcgtc ccgctctata ttcatctcgc cggtagccag ggtcgccagg acgatcagcg
     2521 aatcggcagc gcttttatct ggttggatga tggacagtta cgcatccgga aaatacagct
     2581 ggaagagagt gaagataacg ccagtgtcag cgaacaaact cgacagcaac tgatgactct
     2641 ggccaacgcc ccgttcaatg aggcccttac catccccctg actgacaacg cgcagttgga
     2701 tctcagcttg cgccaactgc tgctgcagct ggtggtcaag cgcgaagcgc tgggcaccgt
     2761 actacgctca cgtagcgaag acatcgggca gtccagtgtt aataccctca gcagtaatct
     2821 gagctataac ttcggcgtct ataacaacca gttgcgtaac ggcgggagca acacctccag
     2881 ctatctgtcg ctgaataacg ttactgcgct gcgcgaacat cacgtggtgc tcgacggctc
     2941 actgtacggg attggtagcg gtcaacagga cagtgaatta tataaagcga tgtatgaacg
     3001 cgattttgct ggtcaccgat ttgccggtgg aatgctcgac acctggaact tgcagtcctt
     3061 agggccgatg accgccattt cagcagggaa gatttacggc ctttcctggg gaaaccaggc
     3121 cagctccacc atcttcgaca gcagccagtc ggccacgcca gtgatcgcct ttttaccggc
     3181 ggcgggcgaa gtacatctca cccgtgatgg gcggctacta agcgttcaga acttcaccat
     3241 gggcaatcat gaagtggata cccggggtct accgtacggt atttacgatg tggaagttga
     3301 ggtaatcgtt aacggtcgcg tgatcagcaa acgcacccag cgggtcaata agctgtttag
     3361 ccgggggcgt ggcgtcggtg caccgctggc gtggcagata tggggcggta gctttcatat
     3421 ggatcgctgg tcggaaaacg ggaaaaagac gcgaccagcc aaagagagtt ggctggcagg
     3481 tgcctcgacc tccggctcac tgagtacgtt tagctgggcg gcaacgggat atggatacga
     3541 taatcaggcg gtgggtgaaa cccgtctgac gctgccgctt gggggggcga tcaacgttaa
     3601 cctgcaaaac atgctggcca gtgacagctc atggagcaac atcgccagca tcagcgccac
     3661 tctacctgga ggcttcagtt cgctgtgggt taaccaggaa aaaacccgca ttggcaatca
     3721 attgcgacgt agcgatgccg acaaccgtgc aatcggcggc acactcaacc tgaactcact
     3781 gtggtcgaag ctgggtacgt tcagcatcag ctacaatgat gaccgccgct acaacagcca
     3841 ttattacacg gcagattact atcaaagtgt ctacagcggt acctttggtt cgcttggcct
     3901 gcgggccggt attcagcgct ataacaacgg cgacagcagc gccaatacag ggaaatatat
     3961 cgctctcgat ctctcgctac cactgggcaa ctggtttagc gcagggatga cccatcaaaa
     4021 cggctacacc atggcaaacc tgtcagcacg caaacagttt gatgaaggaa ccattcgcac
     4081 tgttggtgcc aatctgtcac gagccatctc cggcgatacc ggtgatgaca aaaccctcag
     4141 cggtggggcg tatgcacagt tcgacgctcg ttacgccagc ggaacgctga acgtcaatag
     4201 cgcggcggac ggctacatca atactaattt gaccgccaac ggcagcgtcg gctggcaggg
     4261 taaaaacatc gctgccagcg ggcggaccga tggcaacgct ggggtgatat tcgacactgg
     4321 cctggagaac gatggtcaga tcagcgccaa aatcaacggg cggattttcc cgcttaacgg
     4381 caagcgtaac tatctcccgc tctctcccta tggaagatat gaggtggagt tgcagaacag
     4441 caaaaactca ctcgacagtt acgatatcgt cagcggtcgc aaaagtcatc tgactctcta
     4501 tccaggcaat gtcgctgtca ttgagccaga ggtgaagcag atggttaccg tctccggtcg
     4561 tatccgtgcg gaagacggca cactgctggc taacgcacgg attaacaacc atatcggccg
     4621 aacccgaacc gatgaaaacg gcgagtttgt catggacgtg aataagaaat accccactat
     4681 cgattttcgc tacagtggca ataaaacctg cgaagtggca ctggaactca accaggcgcg
     4741 cggtgccgtc tgggtcggtg atgtggtctg cagcggcctc tcatcgtggg cggcggtgac
     4801 gcagacagga aaaaagaatg agagttaacc tactgatagc gatgataatc tttgcgctaa
     4861 tctggcctgc aaccgcgctc agagcggcag tgagcaaaac aacctgggcg gatgctccgg
     4921 cacgcgagtt tgtgtttgtc gaaaacaact cagacgacaa ctttttcgtc actcctagcg
     4981 gggcgctgga tccgcgcctg accggtgcca accgctggac cggtttaaaa tacaatggtt
     5041 caggaaccat ctatcagcaa agcctcggct acattgataa cggttacaac accggccttt
     5101 ataccaactg gaagtttgat atgtggctgg aaaattcacc agtttcatct cctttaactg
     5161 gcttgcgctg catcaactgg tacgcagggt gtaatatgac caccagtctt atcctgccgc
     5221 aaaccaccga cgccagtgga ttttatggcg cgacggtgac cagcggcggc gcgaaatgga
     5281 tgcacggcat gttgtcagac gcgttttacc agtatctgca acaaatgccc gtcggcagca
     5341 gctttacaat gaccatcaat gcctgccaga cctctgtgaa ctatgacgcc agcagcggcg
     5401 cacgctgtaa ggatcaggcc tccggcaact ggtatgttcg caacgtcacc catacgaaag
     5461 cagcaaatct gcggttgata aatacccact cgctggcgga agtatttatc aacagcgacg
     5521 gagtaccgac tctgggcgaa gggaacgccg actgccggac gcaaaccatc ggcagccgtt
     5581 caggattaag ttgtaagatg gttaactata ccctgcaaac aaacggactc agcaacacct
     5641 caatccatat attcccggcg atcgccaact cgtcgttagc ctcggccgtc ggggcgtacg
     5701 atatgcagtt cagtctgaat ggcagttcat ggaaaccggt gagcaatact gcctattact
     5761 acaccttcaa cgagatgaag agcgcagact cgatctatgt tttcttctcg agcaacttct
     5821 ttaaacagat ggtgaacctc gggatcagcg atatcaacac caaagatcta ttcaactttc
     5881 gctttcagaa caccacatca ccggagtctg gctggtatga attttctacc tccaacacgc
     5941 tgattatcaa accccgtgat ttcagcatca gtattatctc cgatgaatat actcagacac
     6001 cgtcgcggga gggatatgtc ggcagcggcg agtcggcact cgatttcggc tatatcgtaa
     6061 ccaccagcgg taaaacagct gccgacgaag tgctgatcaa ggtgaccgga cccgcgcagg
     6121 tgattggcgg gcgctcctat tgtgtcttca gctccgatga cggtaaggcg aaagtaccgt
     6181 tcccggcgac gctttccttt attacccgca acggagctac aaaaacctac gatgccgggt
     6241 gcgatgatag ctggcgggat atgaccgatg cgctgtggtt gaccacaccg tggactgata
     6301 tctctggcga agtggggcag atggataaga ccacagtcaa attttcgatt ccaatggata
     6361 acgccatttc tctgcgtacg gtagatgata acggctggtt tggcgaagtc agcgcttcag
     6421 gagagattca tgttcaggcg acgtggcgta acattaacta aggccctgct gacagtggtc
     6481 tgtatgctgg cggcaccttt gacacaggcg atttcggtcg gcaatctgac tttttcactg
     6541 ccgtccgaga ctgactttgt cagcaaacgt gtcgtgaata acaacaaaag cgcgcggata
     6601 taccgtattg ccatcagtgc tattgatagc ccgggcagca gtgaattgcg cacccgaccg
     6661 gtggatggtg aactactttt cgccccccgc cagctggcgt tgcaggctgg tgagagcgag
     6721 tattttaaat tttactatca tggcccacgg gataaccgcg agcgctacta ccgggtctca
     6781 tttcgcgagg ttcccactcg taacctgaca aaacgcagcc ctaccggcgg tgaggtcagc
     6841 acggagccgg tggtggtgat ggataccatt ctggtagtac gaccacgtca ggttcagttt
     6901 aaatggtcct tcgatcaggt gaccggaacg gtaagtaaca ccggcaacac gtggtttaag
     6961 ctactgatta aaccaggatg tgattcgacc gaagaggaag gcgatgcctg gtatctacgt
     7021 ccgggagacg tggttcatca gcctgagtta cgtcagccgg ggaatcatta tctggtctat
     7081 aacgacaaat tcattaagat tagcgattcc tgtccggcta agcccccttc ggcggactaa
     7141 gacttatcca tcggacgagg gaagaattcc acctcagaga ctcaaaaatc gtttaaatga
     7201 tgtggaagcg atcgtcaatg tcaccgatga gctttctcat atgcacacca cagtgttcag
     7261 gagacacgga aaggtaccag gcggcctgct aacgagtggt ttcttcacta attaggacgt
     7321 aaaatcggta acggctggaa atcattcaat actcacacta tcgaaagtac accagccaac
     7381 cgcgatacgt ccctgcatac gacgtaccgc agttctcttt acgatttata gctgtactgg
     7441 tgaattatga gcaatctgaa tccatgcatg acgtgtggtg cctgttgtgc atttttccgc
     7501 gtctcttttt actgggccga agccgacgat gctggcggta ccattcccgc caggctcact
     7561 gaacaaatat ccccttttca ccgatgcatg agcggtacta atcagaaaaa tccccgatgt
     7621 attgcccttg caggaacacc gggccaaaat gcctgctgta cgatatatga aaatcgatcg
     7681 tccacatgca gagaattcgc catgtctggt gagaacggag aagtcaatga ggcttgcaat
     7741 cgtgcaagag ctaaatacgg gctaacaccg ttataaatat acaataatta attgcattgc
     7801 cccgccagct gtaatagcgg ggcttcgttt ttcagggaca acaaaacctg aaacttcttc
     7861 cattttccag caacaatgcc tcttcttctg tagcgttctc cggatctaca tcatcctgaa
     7921 gtagcgcgat atgatcactg actgtccgca tcagaatatg aaaagcaacg tttttaacct
     7981 ataaaaa
//
LOCUS       DAZFJL010000174         7940 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFJL010000174 DAZFJL010000000
VERSION     DAZFJL010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.176"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            187..405
                     /locus_tag="ACHJ8C_004206"
     CDS             187..405
                     /locus_tag="ACHJ8C_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO5553973.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQRDEAVGVEELCKQHKE"
     gene            complement(629..1558)
                     /gene="fdhE"
                     /locus_tag="ACHJ8C_004207"
     CDS             complement(629..1558)
                     /gene="fdhE"
                     /locus_tag="ACHJ8C_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO5553974.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLEDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1555..2190)
                     /gene="fdoI"
                     /locus_tag="ACHJ8C_004208"
     CDS             complement(1555..2190)
                     /gene="fdoI"
                     /locus_tag="ACHJ8C_004208"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO5553975.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPAIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2187..3089)
                     /gene="fdoH"
                     /locus_tag="ACHJ8C_004209"
     CDS             complement(2187..3089)
                     /gene="fdoH"
                     /locus_tag="ACHJ8C_004209"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO5553976.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3102..6152)
                     /gene="fdnG"
                     /locus_tag="ACHJ8C_004210"
     CDS             complement(3102..6152)
                     /gene="fdnG"
                     /locus_tag="ACHJ8C_004210"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5565..5567),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO5553977.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSTWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLAANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6346..7179
                     /gene="fdhD"
                     /locus_tag="ACHJ8C_004211"
     CDS             6346..7179
                     /gene="fdhD"
                     /locus_tag="ACHJ8C_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO5553978.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQMFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7267..7821
                     /locus_tag="ACHJ8C_004212"
                     /pseudo
     CDS             7267..7821
                     /locus_tag="ACHJ8C_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
BASE COUNT         1869 a         2017 c         2088 g         1966 t
ORIGIN      
        1 gcccccgctt aattaaaagt aataaaccat ttttgagtaa caattgaaat aactgaaact
       61 gttacattat ctttggatgc cgtctcgcaa aaacaaaaca atatgaaaaa atccgctttc
      121 gttaattaac tttccggcgt aattttcgca catacattaa tgagtaatat atgtaaggtg
      181 gtggtaatgg cgatgaatac ggtttttctt catttatcag aagaggcaat taaacggctg
      241 aataaactca ggggctggcg taaggtttca cgatctgcaa ttttacgcga agcggtagag
      301 caatatctgg agcggcagca atttccggtg cgtaaggcaa aaggcggcag gcaaagggac
      361 gaggcagttg gtgttgaaga actatgtaag cagcataagg aatgacaaaa agattttttc
      421 attcttgaat ataaaaacaa aaaaccagat gaccttattc tggtattaat acaaggttgt
      481 tttacttgaa cttataataa ctgcaactgt tacatcatat ctggaaaacg cctcgcgaaa
      541 tacgagagat tttgcgggaa tagcaggtgt cgtcacaccc tatgaactgt aatccgggcg
      601 accagcgcca cccggaaaac gtcagagatt actccccttc acccggaaac aggaacgggt
      661 tgatggaact gcgggcatag ccttcttgct ccattcgcgc gtccagtacc agagaggcga
      721 ggtcatctgc cacggcttca actttcggtt ctttttcctg ataaagaatc ttcaggtaag
      781 tgccgcagtc atcgcagctt tcggctttaa tcgcggcctg ttcgtcttcc agcgaccagt
      841 aatgcagttt gccgctctgc tcgcagttgc tgcattttac gcgcactacg tgccattcgg
      901 tttcacacag gttgcagtgc aggtaacgca gaccctgagt ggtgccaatt tgcaccatgc
      961 tggaaaccgg catagagcca cataccgggc aatattgacg ttgttcgccg tattcagcgc
     1021 gggctttgcc ggggatcaga ttggccatct gcgcccagta gagcgacagt gcagcccaga
     1081 taaacggcgc tttatcgcta ctgacggatg agaaatcaga ggcaaacagt gcgctggcca
     1141 tatcttccag ctcctgagtc gatgccttct ccagattctc aatcactgcc agcgccgggc
     1201 cgctcatttc aggtttaagc tcagcaatta gcgccatcag cagcttttgc cagtgcttat
     1261 cacgcggcag aacgtgaata tccagcgggg gcttgccttg tgcgctggct tctttaatgc
     1321 gtgcagtcag atccatttcc agcggatggt cgtacagcac cacttcctgg gcgtgggcga
     1381 taagcgcagc aaagcgcagg taatcaccca gcggattatt ttctgccagc tcgcgcagac
     1441 gctcggcgcg gcggttgtat aaattcttga gccgagggaa caataacggc ggaatcatat
     1501 ccgccgtacg tttctcgctc gaacccagct catcttgcgg gattatgcga atactcattc
     1561 agctttcttt tccgttgtct tgcggacctc acggtaccag cgcgggtgat gtttctttgc
     1621 ccatgcgctg gtcacccatc cttccaccat ggcggtaatc gtgcctttca cccaaagggc
     1681 ggcgtagata tgcaccatga taaccacaat taacgccact gcggcaaatg aatgcagcat
     1741 taacgcgaat cggatcgccg ggattgagaa agcaggcgca aaataaggac gccagataat
     1801 cacgccgctc accagcaaca ggaccaggaa aataatcgcc gcccagaaaa cgcatttctg
     1861 accgaagtta taacgcccgg tgtcacctac ttcctcgttg acgacgatct tacgaatatt
     1921 cttcgcccaa aagatatcat cccgattgat taggttgtga tgccagtaac ggaaaaacat
     1981 gatgatgaac gaggcaaaca taaccacgcc gacaaacggg tgcagaatgc gcgccagctg
     2041 cggtgtgccc atgatttgca tcaaccagtt gaaggacggg aacaaaaagc ccagcccgct
     2101 caccgccgcc aggatgaagc agaaggcggt gatccagtgg ttgatacgtt ccggcgcggt
     2161 gtagcgcacg atggtgtcac gtcgtttcat ttgcgctcct cgtctttctc ttcgtgcaga
     2221 ttattctctt cctcatccgc acggttcgga ccgacaccca cgtagtggaa gatactggct
     2281 gcgaaggtag ccgcaaagcc aacagctgcg agcggtttcc agatgccttt ccagaatttc
     2341 acggtttcgc tgatttccgg gttctccggc aagccatgat acagatttgg cttgtcagca
     2401 tggtgcagca cgtacataac atgtgtacca ccgacgcctg ccgggtcgta caaacccgca
     2461 ttgtcgtaac cacgggtttt cagctccgcc acgcgctcgc tcgccagcgt tttcatcgac
     2521 tctttcgtac cgaagtgaat cgcgcccgtt gggcaggtct tcacgcaggc cggttcttgc
     2581 ccaaccacca cgcggtcaac gcacaacgta catttgtaga cgcggttgtc ttccgggttg
     2641 aggcgcggaa tgtcgaacgg acagcccgca atgcaataac cgcagccgat gcactgctcg
     2701 gactggaagt cgacgatacc gttggcatac tgaataattg ccccttccgc cgggcacgct
     2761 ttcaggcagc ccggatcgga acagtgcata cagccgtctt tgcggatcag ccattccagt
     2821 ttgtcgttct gctccacttc cgagaagcgc attaccgtcc atgatttggc acttaaatca
     2881 ttggggttgt cgtatacccc aatgttattg ccgacggtat cgcgaatgtc gttccactct
     2941 gaacacgcca cctgacaggc tttacagccg atacaggtgg taacgtcgat gagtttcgcc
     3001 acttcttcct ggaagtcccg cgcctgaggc gcgggggtca gaccgttagt cgcggaacga
     3061 cgaatgatat cttgcgattg ataagccata tgtcgtctcc gttacacctt ttccacattc
     3121 acaaggaagg acttaaactc cggcgtctgc gtgttcgcat caccgacgaa tggcgtcaac
     3181 gtattggcaa taaagccttt tttcgcaaca ccttcatagc cccagtgaat cggaataccg
     3241 atggtatcga tatctttgcc gtttgctttc agcgtgcgaa tacgtttggt cactaccgct
     3301 ttggctttga tatagccacg gttggaggag actttcacag tatcgccctg ggcaatgcca
     3361 agtttattcg ccagcgactc cccgatttcc acaaactgct ctggttgcaa aatcgcattc
     3421 aacagcgcgt gtttggtcca gtagtggaag tgctcggtca gacgataggt ggttccgaca
     3481 tacgggaact tatcggcttt acccaatgct tcggcgtcgt ctttaaagat acgcgcagcc
     3541 gggttcgaga taacgtttgg atgcagcggg ttagttccca gcggcgtttc aaacggctcg
     3601 tagtgttccg ggaacggacc ttccgccatc ttatcgaggg caaacagacg tcccatgcct
     3661 tcctgctgca tgataaacgg ccctacgccg ctgcccggag gcgctgcgct gtagtccgga
     3721 atatcccagc cggtccactt agtgccgtcc catttcagca actgacgctt cggatcccat
     3781 gggttaccct gcggatctgc ggaggcgcgg ttatacagaa tgcggcggtt aagcggccat
     3841 gcccatgccc agcccagcgt gttaccgagg ccagacggat cggcgttatc acgacgcgcc
     3901 atctggttgc cttccggcgt ccagctaccg gcgaaaatcc agcagccaca ggaggttgta
     3961 ccgtcatcgc gcagttgggc gaacgagcta agttgttggc ctttcttgac gataaccgca
     4021 ccggttgccg ggtcggtaat atcggccagc gctttgccgt tgctctccat tgccacttct
     4081 tctgaagatg gctcatgcgg aatggcgtag ttccaggtca tgttcagcac ctggtccggg
     4141 ttcgcaccac cctgttcggc atacatcttg cgcaagcgca ggaagatacc ggagaggatc
     4201 tcgccatcag tcagcgcaat ccccggggcg tccgcacctt tccagtgcca ttgcaaccag
     4261 cgaccagagt taacgattga accgttctct tccgcgaagc aggtcgatgg cagacggaac
     4321 acttcggtct ggatcttcga cgagtcaact tcgttcagct caccgtggtt ctgccagaag
     4381 ttagaggttt cagtgttcag cgggtcgata gtcacgagga atttcagctt cgacagacag
     4441 ccgatcactt tgtttttgtt cgggaatgag gcaacagggt taaagccctg gcagatatag
     4501 ccattgacct tgccctcttt catcatctcg aagtattgca ggacgtcgta gcctttatcc
     4561 cacttcggca accagtcaaa gccccagcta ttttccgccg tcgctttatc accaaagaag
     4621 gccttcatca ttgagacgaa gaatttcggg tagttgcccc agtagttgac ctggccttcc
     4681 agcagtggtt ttggcgtgtt ggcagcgagg taagtttgca gatcggtctg cttctcgctt
     4741 ggcaacgtca tgtaacctgg cagactctgc gacagtagcc ccaggtccgt cagcccctga
     4801 atattggagt gaccgcgcag ggcgttaacg ccgccgccag ccatccccat attgccgagc
     4861 agcaactgga tcatcgccat cgtacggatg ttctgcgcac cgatggagtg ttgcgtccag
     4921 ccgagggcgt agaggaacga cgctgtttta tcgtgagcac tggtttcggc gatgtattcg
     4981 cagactttca ggaacgcgtc ttttggcgta ccgcagatgt tttcaaccac atctggcgta
     5041 taacgggaaa cgtgctgttt cagcaagttc cacacgcaac gtggatgttg cagggttgta
     5101 tcacgtttgg cgaagccgtt ttcgtccagt tcgtaagtcc aggtggactt atcgtacttg
     5161 cgtttttccg cgtcgtagcc ggtgaacagg ccatcttcaa agccgtaatc ctcacgcacg
     5221 atcaggctgg cgttggtata ggcttcggtg tattcgcggt tgaatttttc attgttcagc
     5281 aggtacagca atacgcctga caggaaagca atgtcagtac cggaacggat aggggcatag
     5341 tagtcagcca ccgccgctgt acgtgtaaag cgaggatcga taacaatcag ctttgcgccg
     5401 ttgtgaattt tggcttccat cgcccagcgg aacccgaccg ggtgagcttc agcggcgtta
     5461 ccgcccatca ccacgacgag gttggcattc ttgatgtcga cccagtggtt ggtcatcgca
     5521 ccgcgaccaa atgttggagc aagacttgct accgttggtc cgtgtcagac acgcgcctgg
     5581 ttgtcgaccg cgagcatacc cagcgcgcgg gagaattttt gcgttaaata gccggtttcg
     5641 ttactcgacg cggaagcaca cagcatcccg gtggagagcc agcggttaac agtcacgcct
     5701 tcggcgtttt gcgcaatgta gttagcatcg cggtcttctt tcatcagttt ggcgatgcga
     5761 tcaaacgcct cttcccaact gatttgttgc catttatcag aacctggcgc acggtattcc
     5821 ggaaacttca gacggctttc ggagtggatg aaatccacca ggccagcgcc tttcggacaa
     5881 agtgctccgc ggttgaccgg gtgatccggg tcaccttcga tatggaagat agatgctttg
     5941 gcgttttttg ctccgtcacc gaggctgtac atcaacagcc cacagcctac ggaacaatag
     6001 gtgcaggtat tacgggtttc gcgggtgcgc agcagtttat actgccgggt ttccgcgagt
     6061 gctacgctgg gtgcaaaacc cagtgccgct gccgtggtgc ctgccatacc gccagcgcag
     6121 atcttaaaga actgccttct gctgacctgc atggattgct ccttgtttcg acattgtcac
     6181 gtcccattta cattcgcttg ctgcgtgtgc agggagtggg agttattttt ctttgcggaa
     6241 ggggccgcaa aggtccagaa ttggctcaat ttccctccat ccaggaaggg tttgtaacag
     6301 aataccataa tgttggtgtg tgtgttctta tctggttaat agaaagtgaa aaaaacacag
     6361 caaaaagaaa tcgagaatgt gacaaatatc acaggtgttc gtcaaattga gttatggcgg
     6421 cgtgatgatt tacaacaccc acggttagat gaggtggcgg aagaagttcc cgttgcgctg
     6481 gtctacaacg gcatttcgca cgtggtgatg atggcgtcgc ccaaagacct tgagtacttt
     6541 gcgctcggtt tttcgctttc cgaaggaatt atcgaaagcc cgcgcgatat cttcggcatg
     6601 gatgtcgttc cttcctgtaa tggcctggaa gtacaaattg agctttccag ccgccgcttt
     6661 atggggttga aggagcgccg ccgggcgctg gcgggacgta cgggctgtgg cgtatgcggt
     6721 gtagaacagc ttaacgatat cggtaaaccg gtgcagccgc tgccgttcac ccagatgttt
     6781 gatctcaaca aactggatga tgcattacgt catctcaatg atttccagcc agtggggcaa
     6841 ctgactggtt gtactcacgc cgctgcatgg atgttgccat ctggcgaact ggtcggcggg
     6901 catgaagacg tgggtcgcca tgtggcgctg gacaaactgt taggccgccg gtcacaagaa
     6961 ggggaaagct ggcaacaagg tgcggtgctg gtttccagcc gtgccagtta tgaaatggtg
     7021 caaaagtcgg cgatgtgcgg cgtagagatt ttgtttgcgg tgtctgccgc gaccacactt
     7081 gctgtagaag tggctgagcg ctgtaatctg acgctggtgg gcttttgtaa accgggtcgg
     7141 gcaacggttt atacccatcc gcagcgtttg agcaattaat taaaataagt cctgcgatat
     7201 atattaaatt acaggactta ttcatttcgt gaattttgtt attttttcat aaacaaaacg
     7261 cgtgtagtgg cgttaacgcc tgcgctgcaa cctattgatg gcgtggcggt gagttatatt
     7321 gatgctgccg tagcgttggg taataccatt aacgaaatgg ataaatatta cacccaggaa
     7381 aattataaag acgatgcgtt tgcgaaaggg aaaacattcc actactactc tctggcagtc
     7441 atgatttcag cgaaacaaat taataacctg atatcgcaag ataagtttga tgcagaagcg
     7501 gcaatgaaga aagtgtctga actggaaacg ctggtggcgc aagccaaaga agcggataaa
     7561 agcggcatga atttctcgtt tattaattcg gcaggccagt atcaactcga ggctaaaaaa
     7621 tacgttcgcc gcatcagaga taaagtcctg cactctgact gggataaaga gcaacttcag
     7681 gacgcaaact caagctggat ggccgaagac tcatttccac gagcgttatg cgattacaac
     7741 gaaatggtcg atgaaatatt acagttaatt gtaatcgcgg ggcgggtttg tgatgagcac
     7801 ggatatgtca cgaagtcgta acaatataaa gcagagtgaa ggataaattt tgtgaataaa
     7861 gtatcattat ttaatgattt tgtgaatgtt aaaatgtctc agataatttg tttaagcgtt
     7921 atttatattt gtggcttatg
//
LOCUS       DAZFJL010000175         7851 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZFJL010000175 DAZFJL010000000
VERSION     DAZFJL010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.177"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            112..201
                     /gene="kdpF"
                     /locus_tag="ACHJ8C_004213"
     CDS             112..201
                     /gene="kdpF"
                     /locus_tag="ACHJ8C_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO5553979.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            201..1874
                     /gene="kdpA"
                     /locus_tag="ACHJ8C_004214"
     CDS             201..1874
                     /gene="kdpA"
                     /locus_tag="ACHJ8C_004214"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO5553980.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGMTGVER
                     VLFSALGVSDREMNWKQYLSAILGLNILGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLAPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSAHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLLVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLALCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1897..3945
                     /gene="kdpB"
                     /locus_tag="ACHJ8C_004215"
     CDS             1897..3945
                     /gene="kdpB"
                     /locus_tag="ACHJ8C_004215"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO5553981.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGVMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVEEKALA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3954..4526
                     /gene="kdpC"
                     /locus_tag="ACHJ8C_004216"
     CDS             3954..4526
                     /gene="kdpC"
                     /locus_tag="ACHJ8C_004216"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO5553982.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPNASTNVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4519..7203
                     /gene="kdpD"
                     /locus_tag="ACHJ8C_004217"
     CDS             4519..7203
                     /gene="kdpD"
                     /locus_tag="ACHJ8C_004217"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO5553983.1"
                     /translation="MNNEPLRPDPDRLLEQAAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLKGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSSQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTQWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFEQVLINLLENAVKYAGAQAE
                     IGINAHVEGENLQLDVWDNGPGLPPGQEKTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7200..>7851
                     /gene="kdpE"
                     /locus_tag="ACHJ8C_004218"
     CDS             7200..>7851
                     /gene="kdpE"
                     /locus_tag="ACHJ8C_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO5553984.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATTTPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITET"
BASE COUNT         1608 a         2076 c         2334 g         1833 t
ORIGIN      
        1 gcttttgcca tttttatact ttttttacac cacgcccgca gatttttgcg aaatctttgc
       61 agccagaact ctacccttcc ggtatcactt ttaggccact ggaggtgcac tgtgagtgca
      121 ggcgtgataa ccggcgtgtt gctggtgttt ttattactgg gttatctggt ttatgccctg
      181 atcaatgcgg aggcgttctg atggctgcgc aagggttctt actgattgcc acgtttttac
      241 tggtattaat ggtgctggcg cgtcccttag gtagcgggct ggcgcggctg attaatgata
      301 tacctcttcc tggtatgacg ggcgttgaac gcgtactctt tagcgcgctt ggcgtctctg
      361 accgcgagat gaactggaag cagtatctct cagccattct tggcctgaat atactggggc
      421 tggcggtgct gttttttatg ttgctcggtc agcactatct gccgcttaat ccacagcagt
      481 tgccggggct gtcgtgggat ctggcgctca ataccgccgt cagctttgtc acgaacacca
      541 actggcaatc ttatagcggt gaaaccacgt tgagctattt cagccagatg gcgggcttaa
      601 cggtgcaaaa ctttctttct gccgccagcg ggattgcggt gatttttgcg cttattcgcg
      661 cgtttacccg ccagagcatg agcacgctcg gtaatgcctg ggtcgatctg ctacgcatca
      721 cgttatgggt gttagcccct gtggcgttgt tgatcgcgct gttttttatt caacaaggtg
      781 cgctgcaaaa ctttcagccc tatcaagcgg tgaataccgt tgaaggagcg caacagctgt
      841 taccaatggg tccggtggct tctcaggaag cgatcaagat gctcggtact aacggcggtg
      901 gcttctttaa tgccaactcg gctcatccgt ttgaaaaccc gaccgcgctg accaacttcg
      961 tgcaaatgct ggcgattttc ttgatcccaa cagcgctgtg ctttgccttt ggtgaagtgg
     1021 cgggcgatcg tcgccagggg cgcatgttgc tgtgggcgat gtcggtgatt tttgtcatct
     1081 gcgtaggcgt agtgatgtgg gcagaagttc agggtaatcc tcatctgctg gcgctcggcg
     1141 cggacagcag catcaatatg gaaggtaaag agagccgttt cggcgtgctg gtcagtagcc
     1201 tgtttgcggt cgtgactacg gcggcttcct gcggcgcggt gattgcgatg catgattcgt
     1261 ttaccgctct tggtggcatg gtgccgatgt ggttgatgca aattggtgaa gtggtattcg
     1321 gcggcgtcgg ttccggcctt tacggcatga tgctgcttgt cctgctggcg gtgtttattg
     1381 ccgggctgat gattggtcgt acaccggaat atctgggtaa aaaaatcgac gtacgcgaga
     1441 tgaaactgac tgcactggcg attctggtca ccccgacgct ggtgctgatg ggcgcggcgc
     1501 tggcaatgat gaccgacgcc ggacgtagcg ccatgctcaa ccctggcccg catggtttta
     1561 gcgaagtgct gtacgccgtg tcatccgccg ctaacaacaa cggcagcgcc tttgccggat
     1621 taagcgccaa ctctccattc tggaactgtt tactggcgtt gtgcatgttt gtcgggcgct
     1681 tcggggtgat tatcccggtg atggcaattg ccggttcgct ggtgagtaaa aagagccaac
     1741 ccgccagctc cggcacattg ccaacgcacg gcccgctgtt tgttggcctg ttaatcggca
     1801 ccgtgttgct ggttggcgca ctgaccttta tccctgccct ggcgcttggt ccggtggcgg
     1861 aatatctctc ctgatgatat tgagtgagca ctgaatatga gtcgtaaaca actggcgcta
     1921 ttcgaaccaa cacttgtcgt tcaggcgctg aaagaagcgg tgaaaaaatt aaacccgcag
     1981 gcgcaatggc gcaatccggt gatgtttatc gtctggatcg gcagtctgct gaccacctgt
     2041 attagcatcg cgatggcaag cggtgtgatg cctggcaatg cgctgtttag cgcggcaatt
     2101 agcggctggc tgtgggtcac cgtactgttc gctaatttcg ccgaggcgtt ggcagaaggc
     2161 cgcagtaaag cgcaggccaa cagtctgaaa ggggtgaaaa aaaccgcttt tgctcgcaaa
     2221 ctgcgcgaac cgaaatatgg cgctgcggcg gacaaagttc ctgctgatca actgcgtaaa
     2281 ggtgacatcg tgctggtaga agctggcgat attatcccct gcgatggtga agtcattgaa
     2341 ggcggcgcat cggtcgatga aagcgccatc accggggaat cggctccggt gatccgtgaa
     2401 tccggcggtg attttgcctc cgtcactggc ggcacgcgta ttctttctga ctggctggtg
     2461 attgagtgca gcgttaaccc cggcgagaca tttctggatc ggatgatcgc gatggtggaa
     2521 ggtgcgcagc gacgtaaaac gccgaacgag attgccctga ccattctcct gattgccctg
     2581 actatcgtct ttttactggc aaccgccacg ctgtggccgt tttccgcgtg gggcggtaat
     2641 gcagtcagcg taacggtact ggtggcactg ctggtctgtc tgatcccaac cactattggc
     2701 ggcctgttgt cagcgattgg cgtcgccggg atgagtcgga tgctgggcgc gaatgtgatt
     2761 gccaccagcg ggcgagcagt tgaagcagcg ggtgatgttg acgttctgct gctggataaa
     2821 accggcacca tcaccctcgg taaccgtcag gcgtcagagt ttatccccgc acaaggcgtg
     2881 gaggaaaaag cgctggccga cgccgcacaa ctggcttcgc tggctgatga aacgccggaa
     2941 ggccgcagta ttgtgatcct cgccaagcag cgttttaacc tgcgcgagcg cgatgtgcag
     3001 tcgctccatg ccacctttgt accttttact gcacaaagcc ggatgagcgg gatcaacatc
     3061 gacaaccgca tgatccgtaa aggttctgtc gatgccattc gccgccatgt tgaggctaac
     3121 ggcggtcact tccctgccga tgttgatcaa aaagtcgatc aggttgcgcg tcagggagcc
     3181 acgccgctgg tggtggtgga aggttctcgt gtgctgggtg ttattgcgct gaaagatatc
     3241 gtcaaaggcg gtattaaaga gcgcttcgcc cagctgcgta aaatgggcat taaaacggtg
     3301 atgattaccg gcgataaccg tctgactgcc gccgcgattg ccgcggaagc gggcgtcgat
     3361 gattttctcg ctgaagcgac gccagaggcc aagctggcgt tgattcgtca gtatcaggca
     3421 gaaggccgtt tagtggcgat gaccggcgat ggcaccaatg atgctccagc gctggcgcag
     3481 gcggatgtcg cggtggcgat gaactccggc acccaggcgg cgaaagaagc aggcaatatg
     3541 gtggatctcg actccaaccc gaccaagttg atcgaggtgg tgcacattgg caaacagatg
     3601 ctgatgaccc gtggctcgtt gaccaccttc agcattgcca acgatgtggc gaaatacttc
     3661 gccattattc cagcggcgtt cgcggcaacg tatccgcagt taaatgcgct gaacatcatg
     3721 cgcctgcatt cgcccgactc cgccattctc agtgcggtca ttttcaacgc cttgattatc
     3781 gtctttttga ttcccctggc gttaaaaggc gtgagttata aaccgcttac cgcttctgcc
     3841 atgttgcgcc gtaacttatg gatttacggt ctgggtgggc tgctggtgcc gtttattggt
     3901 atcaaagtca ttgatttact gctaaccgtt tgcggtctgg tgtgaggttt accatgagtg
     3961 gattacgtcc ggcattatcg acatttctct ttctgttatt gattactggc ggcgtttacc
     4021 cgctgctgac caccgcactg gggcaatggt ggtttccctg gcaggccaac ggttcattga
     4081 ttcgtgaagg tgatacggtg cgcggttcgg cattaatcgg gcaaaatttt accggcaacg
     4141 gctattttca tggtcgcccg tcggcaacag cagaaatgcc ctataatcca caggcttctg
     4201 gcgggagcaa cctggcggtc agtaacccgg agctggataa acagatagcc gcacgcgttg
     4261 ctgcattacg ggctgctaac ccgaatgcca gcacgaacgt tccggttgaa ctggtgacgg
     4321 cgtcagcaag cgggctggac aataacatca ccccgcaagc ggcggcctgg cagatcccac
     4381 gcgtggcgaa agcgcgtaat ctcagcgttg aacagctcac gcaactgatc gcaaaataca
     4441 gccaacaacc gctggtgaaa tatatcggcc agccggtcgt caacattgtc gaactcaatc
     4501 tggcactgga taagcttgat gaataacgaa cccttacgtc ccgaccccga tcgtctgctg
     4561 gaacaagctg ccgcgccgca tcgggggaag ctgaaagttt tcttcggtgc ctgcgcaggc
     4621 gtcgggaaga cctgggcgat gctggcggaa gcccagcgac tgcgggcgca agggctggat
     4681 attgtggttg gcgtggtaga aacccacggt cggaaagata ccgccgcaat gctgaaaggt
     4741 ctggctgttc tgccgttaaa acgccaggcg taccgtgggc ggcacatcag cgagtttgat
     4801 ctcgatgccg ccctcgcccg ccgtccggca ctgatcttaa tggacgaact ggcgcacagt
     4861 aatgcgccag gttctcgtca tcctaaacgc tggcaggaca tcgaagagct gctggaagca
     4921 ggcattgatg ttttcactac cgtcaacgtt cagcatctgg aaagcctgaa cgatgtggtc
     4981 agcggcgtca ccggaattca ggtacgggag accgtgcccg atcctttttt tgatgccgcc
     5041 gacgacgtgg tgctggtgga cttgcccccg gacgatctgc gccagcgact gaaagaaggc
     5101 aaagtctata ttgccgggca ggcggagcgc gccattgaac atttttttcg caaaggcaat
     5161 ctgatcgccc tgcgcgaact ggcactgcgc cgtactgccg atcgcgtaga tgagcaaatg
     5221 cgcgcctggc gggggcatcc tggcgaagag aaagtctggc acacacgcga cgcgatccta
     5281 ttatgcatcg ggcataacac cggcagtgag aaactggtcc gcgcggcggc gcggttggcc
     5341 tcgcgcctgg gcagtgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     5401 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     5461 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     5521 cataatctcg gcaagattat tctcggtcgc ccggcttcgc gccgctggtg gcgtcgggaa
     5581 acatttgctg accggctggc acgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     5641 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcttt taaagacaag
     5701 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     5761 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     5821 ggcgtcgtgg tggtggcact attttacgga cgctggcctt cggtggttgc caccgtcatt
     5881 aatgtggtga gtttcgatct cttttttatc gccccacgcg gcacgctcgc cgtctctgat
     5941 gtgcaatatc tgctgacctt cgcggtgatg ttaaccgtcg ggctggtgat cgggaacctt
     6001 actgctggcg tgcgttatca ggcgcgggta gcccgttacc gcgaacaacg cacacggcac
     6061 ttatatgaaa tgtccaaagc tctggctgtg ggccgtagtt cgcaggatat cgccgccacc
     6121 agcgaacagt ttattgcctc cacgtttcat gcccgtagcc aggtgttgct gcctgatgac
     6181 aacggtaaat tgcagccttt aacacatccg caaggaatga cgcagtggga cgatgccatc
     6241 gcgcagtgga gttttgataa aggcctgcct gcgggcgcgg gcaccgacac gttacccggt
     6301 gtaccgtacc agattttgcc gctaaaaagc ggcgagaaaa cctacggact ggtggtggtg
     6361 gaaccgggga atctgcgcca gttgatgatc ccggaacagc agcgcctgct ggagacgttt
     6421 acgctgttag tcgccaatgc ccttgagcga ctgacactga ccgccagcga agaacaggcg
     6481 cggatggcaa gcgaacgtga acagatccgc aacgccctgc tggcggcgct ttcgcatgat
     6541 ttacgcacgc cgcttacggt gctgttcggt caggcagaaa tcttaacgct cgatctggca
     6601 agcgaaggat cgccccacgc ccgccaggcc agcgagattc gtcagcatgt gctgaacacc
     6661 acccgactgg tgaataatct actggatatg gcgcgaattc agtccggcgg ctttaatttg
     6721 aagaaagagt ggttaacgct ggaagaagtg gtcggcagcg cgctgcaaat gctggaaccg
     6781 ggtttatcgt cgcccatcaa tctttctctg ccagagccgc tgaccttaat ccacgttgac
     6841 gggccactct ttgaacaggt gctgattaac ctgctagaga acgcggtgaa atatgcgggg
     6901 gcgcaggccg aaattggtat caatgcccac gttgagggcg aaaatctaca actggatgtc
     6961 tgggataacg gccccggtct tccaccaggc caggagaaga cgatatttga taagtttgct
     7021 cgcgggaata aagagtcggc ggtaccgggt gtagggcttg ggttggcaat ttgtcgggcg
     7081 atagtggatg tacacggcgg caccattacc gcattcaacc gaccggaagg tggtgcctgt
     7141 tttcgtgtta cacttcccca gcaaacagcc cctgaacttg aagaatttca tgaggatatg
     7201 tgacaaacgt tctgattgtt gaagatgaac aggctattcg tcgctttctg cgcacggcgc
     7261 tggagggcga cggtatgcgc gtctttgagg ccgaaacgct gcaacgcggc ttgctggaag
     7321 cggcaacccg taagccagat ttgattattc tcgatctcgg cctgcccgac ggtgatggga
     7381 ttgagtttat ccgcgacctg cgccagtgga gcgcggtgcc ggtgattgtg ctttccgcac
     7441 gcagcgaaga gagcgataaa atcgccgcgc tggatgccgg agcggatgat tatctgagta
     7501 agccgtttgg cattggcgaa ttgcaggccc gtctgcgcgt cgcattacgc cgccactccg
     7561 ccaccaccac gcccgatccg ctggtgaaat tttctgatgt taccgtcgat ttagccgccc
     7621 gcgttatcca ccgcggcgag gaagaggtgc atctcacacc gattgagttc cgcctgctgg
     7681 ccgtgctgct caacaacgcc ggaaaagtgc tcacccagcg ccagttactc aatcaggtgt
     7741 ggggaccaaa cgcggtcgaa cacagccact atttgcgtat ttatatgggg catctgcgac
     7801 aaaaactgga gcaagatccc gcccgccccc gccattttat tactgaaacc g
//
LOCUS       DAZFJL010000176         7784 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFJL010000176 DAZFJL010000000
VERSION     DAZFJL010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.178"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..1636)
                     /gene="hutH"
                     /locus_tag="ACHJ8C_004219"
     CDS             complement(<1..1636)
                     /gene="hutH"
                     /locus_tag="ACHJ8C_004219"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HFO5553985.1"
                     /translation="MKGLIRRVTVTVVLWSVCSIAMAKSTITLDGEHLSIDQAWSIAL
                     GDNGVKIASNAMERMEKSYDLVMTAAHSGTPVYGLTVGVGLNKDQPIFDAKGNLTPEV
                     IDASKKFNAAALRAHSAGVGPMLPDYLTRLSMVLRLNTMLHGQTGAQPYIAELYAEFI
                     NRGVTPLIPARGSIGAADIMLASHVGLVMMGEWKARVNGVEMSGGDALVKVGLKPLVP
                     QGKDMLAILSNNSVGTAYAIEAVRSARQVLNVSPVVFAMSLEALNGNVAPVLPQSIAV
                     RPFPQLADSAKEIRDALSGSYLWHKHDKRALQDPLSFRTTVYTISEAMRALRDAEDTI
                     TVQINSSDDNPATVLNADKAYQDSSQVAQYFVKDGAISGGIFPTANFESLPVALATQR
                     LTVALVHVSHNSMRRSLHLEDDHFTGLSRFLSAAGNKGHGFGSLHIPFVALHAENVDL
                     ANPVSFDIQPVAGGIEDTGANNDHAARRLKQVVDNLNVIYGIELMHSAQALDLRLQAD
                     PQLALGKSTKAMFTEYRKVVPFVDQDRVFTPDIAASQ"
     gene            complement(1687..2637)
                     /gene="hutG"
                     /locus_tag="ACHJ8C_004220"
     CDS             complement(1687..2637)
                     /gene="hutG"
                     /locus_tag="ACHJ8C_004220"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050415 - formimidoylglutamase activity
                     [Evidence IEA];
                     GO_process: GO:0019556 - L-histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HFO5553986.1"
                     /translation="MKQTFMAWSGRVDSQETGVSTRWHQHVKPFTEQSHGGSTLIGFA
                     VDDGVQRNGGRAGAAGGPGALRSMLGNMPVLHEGAIFDMGDVQSEDHALEPAQNRLAE
                     NVSTAIARGSLPVVLGGGHEVAWGTFQGIMQSRPDIQRLLIVNLDAHFDLRMAEQANS
                     GTPFRQMQEWNAQHGKPFNYRVLGISRFANTQALFDRADCLGVRYWLDESLQFERGLH
                     EIANALSADIQACDAIYLTICLDVLPGYQAPGVSAPSPLGVPLNITEAIVNLIAGSEK
                     LIAADIAELNPDLDRDNLTAKVAARLVTSIVRRGKTDRTN"
     gene            complement(2637..3848)
                     /gene="hutI"
                     /locus_tag="ACHJ8C_004221"
     CDS             complement(2637..3848)
                     /gene="hutI"
                     /locus_tag="ACHJ8C_004221"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0019556 - L-histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HFO5553987.1"
                     /translation="MSAFTLWRNTTLTTLSANDWGVIENGALISRGDSIVWLGKATQT
                     PRELTADITEEHDLQGKLLTPGLVDCHTHLVYGGQRANEFEMRLQGVAYEDIARAGGG
                     ILSTVNATRAASETQLFELAAQRLQNWLREGVSTLEIKSGYGLTLEDEARCLRVIRRL
                     QESTGLRIQSTFLGAHSLPPEFAGRSDDYITALCDWMGVLHEQGLIDAVDAFCENIAF
                     SPDQVRRVFSKAQHYGLPVKLHAEQLSDQQGAALAAAFSALSCDHLEYLSESGIAAMK
                     AAGTVAVLLPGAYYFLRETHLPPIVALRAAGVPIAISTDHNPGTSPNMSLPLMINMAC
                     TLFRMTPLEAVQGVTRNAAKALGLIDRGELAPGLRADMAVWDLNHPRELAYWFGHQPC
                     LGLIVGGKRSW"
     gene            complement(3845..4426)
                     /locus_tag="ACHJ8C_004222"
     CDS             complement(3845..4426)
                     /locus_tag="ACHJ8C_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HFO5553988.1"
                     /translation="MLISAMDKPLQPWRNGGGFTRQLLAWPDDENWLLRISMANVSQD
                     GPFSAWPGVVRGLAILDGEGVKLTFADSIINLQRHDPALIFPGEAMPDCQLLGGGVRD
                     LNVMAREGDAGMRRVQSVIDWLPPAHAQAGLFTLCEGEWISGENRVFVTANTLLWRFA
                     SDGNSWRFVPRQKVSAGELAGWWIYYLRGEKSL"
     gene            complement(4437..5846)
                     /locus_tag="ACHJ8C_004223"
     CDS             complement(4437..5846)
                     /locus_tag="ACHJ8C_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO5553989.1"
                     /translation="MESSSPIRSKKPVLESNTINAVPESARHGSPSSQFTLWLSANLQ
                     ITAVVDGALAIIFGSEAMSAIIGLLIGNIIGGAVMALHSSQGAKLGLPQMISSRIRFG
                     VKGAALPLLMVIIMYLGFAATGTVLSGQAINRIFGFESASTGIIIFGMLTAMIALVGY
                     KLIHVVGRVAGILSILGFIYLATQLSGHYDVAAAFGQKPFSLSVFLLTIALSAGWQLT
                     FAPYASDYSRYLPSDTPAKSIFFATFLGTTLGAQCAMTFGVLVAACGANFLKDQVGFM
                     GELAGPTFAILIYTVIVCGKLTVNCLNAYGGFMSILATVSAFNNRSQVSQRVRAAYVV
                     GFVMLSMVVALAASADFLNNFKNFVLLLLTVFVPWSAINLIDYYFISQGKTDIPALYT
                     PEGRYGAYNKTALVCYGIGVLVQIPFLNQKLYQGPISVLMDGADISWIVGLLITAVIY
                     YPWAKRTTCVPAYTIYPES"
     gene            complement(6009..7706)
                     /gene="hutU"
                     /locus_tag="ACHJ8C_004224"
     CDS             complement(6009..7706)
                     /gene="hutU"
                     /locus_tag="ACHJ8C_004224"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HFO5553990.1"
                     /translation="MSKPSDPRIDTSRVIHAPHGTQLHCKNWQIEAAYRMLQNNLDPD
                     VAENPQHLVVYGGIGRAARNWECFDKILESLKNLEADESLLVQSGKPVGVFKTHTDAP
                     RVLIANSNLVPQWANWDHFNELDRKGLFMYGQMTAGSWIYIGSQGIVQGTFETFVEAG
                     RQHYNNSLSGKWILTAGLGGMGGAQPLAATLAGACSLNIECQQSSIDFRLRTRYVDKQ
                     AKDLDDALALIKYHTERKDAVSIALLGNAAEIVPELVKRARQGAIKPDLVTDQTSAHD
                     LVYGYLPMGWSVEQWRAAQQDPQQHARLQKEAAQSCVAHVQGMLDFQAMGIPVVDYGN
                     NIRQVAFNEGLQNAFDFPGFVPAYIRPMFCEGKGPFRWVALSGDPEDIYKTDAKIKEL
                     FPNNVLTHRWLDMARERIAFQGLPSRICWLGLGERHLAGLAFNEMVRNSELKAPIVIG
                     RDHLDTGSVASPNRETESMLDGSDAVSDWPLLNGLLNTAGGATWVSLHHGGGVGMGYS
                     QHAGMVIVADGSEEAEKRLARVLVNDCASGVMRHADAGYEAAIECAKNYGLKLPMIR"
BASE COUNT         2000 a         2194 c         1824 g         1766 t
ORIGIN      
        1 tttgcgaagc tgcaatatct ggggtaaaga cccgatcctg gtcgacaaac ggcacgactt
       61 ttctgtattc ggtaaacatt gctttggtgg atttccctaa tgccagttgt ggatcggcct
      121 gcaaccgtaa atcgagagcc tgtgcggaat gcatcagttc aatgccataa atcacgttca
      181 gattatcgac aacctgtttc aatcttctgg ccgcgtggtc attattggca ccggtatctt
      241 ctatacctcc ggcaaccggt tgaatatcga aagaaaccgg gttagcgaga tcgacatttt
      301 ccgcatgcag cgcaacaaac gggatatgta atgaaccgaa gccatgacct ttattacctg
      361 ctgccgacag gaagcgtgac aaaccggtaa agtgatcatc ttcaagatgc agcgagcggc
      421 gcatactgtt atgtgaaaca tgcactaacg ccacggtcag acgctgggtg gcaagcgcaa
      481 ccggtaacga ttcgaaattc gccgtcggga agatcccacc gctgattgcc ccatctttaa
      541 caaaatattg cgcgacctga ctggagtcct gatacgcttt gtctgcattc agcacggttg
      601 ccgggttgtc atcggaactg ttgatctgca cggtaatggt atcttcagca tcgcgcaacg
      661 cgcgcattgc ctcagaaatg gtatacaccg tcgttctgaa cgataacgga tcctgcaatg
      721 cacgtttgtc atgcttatgc cataaataag aaccacttaa cgcatcgcga atctctttag
      781 cggaatcagc caactgcggg aaaggacgca cggcgatcga ctgcggcagt accggcgcga
      841 cattgccatt caacgcttcc agactcatag cgaaaaccac cggactgacg ttcagcacct
      901 ggcgtgccga ccgtaccgct tcaattgcat atgctgttcc gacactgttg ttcgacaaaa
      961 tcgccagcat atctttaccc tgcgggacca gtggtttaag ccccactttc acaagcgcat
     1021 cgccaccact catttccacg ccattaaccc gcgctttcca ctcgcccatc atcaccagac
     1081 caacgtgtga agcgagcata atatccgctg cgccgatact tccccgcgcc ggaattaacg
     1141 gcgtaacgcc tcgattaata aactcggcat acaactcggc aatataaggc tgcgcgccgg
     1201 tttgcccatg caacatagta tttaaacgta acaccattga gagacgggta agataatccg
     1261 gcaacatcgg accgacgccc gcagaatggg cgcgcagcgc ggcagcatta aattttttcg
     1321 atgcgtcaat cacctcaggc gttaaatttc ctttggcatc aaatatgggc tgatctttat
     1381 ttagccccac gcccacggtt aagccataaa caggcgtacc tgaatgcgcg gcagtcatca
     1441 ccagatcata acttttttcc atgcgctcca tggcgttcga cgcgattttg acgccgttat
     1501 cacctaatgc aattgaccag gcctggtcaa tggaaagatg ttcgccatcc agagtaatgg
     1561 ttgatttcgc catcgcaatg gagcaaacgc tccagagcac aacggtgaca gtgactcttc
     1621 gtatcaatcc tttcattgtg tttccttatc atgatgagtt taacaacggg ttactgcgac
     1681 aacgttttaa tttgttctat ctgttttccc ccgccggaca atactggtca ccaggcgagc
     1741 ggcaactttc gcagttaaat tatcgcggtc caggtccggg ttaagttcgg caatatccgc
     1801 agcaatcaac ttctcgctac cggcgattaa attcacaatg gcttcagtaa tatttaacgg
     1861 cacgcctaac ggtgatggag cagaaacacc gggcgcctga taacctggta aaacatcgag
     1921 acaaatcgtt aaataaatgg cgtcacaagc ctgaatatct gccgagagcg cattagcgat
     1981 ctcatgcaaa ccacgttcaa attgcagaga ttcgtccagc caataacgca caccgagaca
     2041 atcagcgcga tcaaataacg cctgagtatt ggcaaaacga cttattccca gcacgcgata
     2101 attaaacggt ttgccatgct gcgcgttcca ctcttgcatc tgacgaaacg gcgtacctga
     2161 attggcttgt tccgccatcc gtaaatcaaa atgcgcgtca aggttcacaa tcagtaaccg
     2221 ctgaatatcc ggacgcgact gcataatccc ctgaaatgtt ccccacgcca cttcatgacc
     2281 gccgcccaga acgaccggca aactgccgcg cgcaatggct gtagatacgt tttccgccag
     2341 acggttttgt gcgggttcca gcgcgtggtc ttcgctttgc acgtcaccca tgtcaaaaat
     2401 agcgccttca tggagcactg gcatattgcc caacatgctg cgtaatgcac ccggtccgcc
     2461 tgctgcgccc gctcgcccac cgttacgttg caccccgtca tcgacggcaa aaccaatcag
     2521 cgtactgcct ccatgacttt gttcggtaaa cggttttaca tgctgatgcc agcgggtgct
     2581 gacgccagtt tcctgcgagt caacacgtcc actccatgcc ataaatgttt gcttcattac
     2641 caactccttt ttccgccgac aatcagcccc agacaaggct gatgtccaaa ccagtaagcc
     2701 agttcacgtg gatgattaag atcccagacc gccatatccg cccgtaatcc cggtgcaagt
     2761 tcaccccggt caataagtcc aagtgccttt gccgcattac gcgtaacgcc ctgaacggct
     2821 tccagcggcg tcatgcgaaa cagcgtacag gccatattga tcatcaatgg caatgacata
     2881 ttgggagacg ttccgggatt atggtccgtg gagatagcaa tcggcacacc tgcggcccgc
     2941 agagcgacaa tcggcggcag atgggtttcg cgcaggaagt aataagcacc agggagcagt
     3001 accgccaccg ttccagccgc tttcatggcg gcgatccccg actcgctaag gtattccaga
     3061 tgatcgcagg agagtgcaga aaacgccgct gccagcgccg cgccttgctg gtcagaaagc
     3121 tgttcggcat gcagttttac aggtaatcca tagtgttgcg ctttactgaa aacgcgccgt
     3181 acctggtctg ggctaaaggc gatgttttca caaaaagcgt caaccgcatc aatcagcccc
     3241 tgttcatgta agacgcccat ccagtcgcaa agcgccgtaa tgtaatcatc actgcgcccg
     3301 gcaaactctg gcggcaagct gtgcgcccca agaaacgtag actgaatacg taatcctgta
     3361 gattcctgta agcggcggat aacgcgcaaa cagcgcgctt catcttccag cgtcaagcca
     3421 taaccggatt ttatttccag cgtactgacg ccttcacgca accagttttg tagtcgctgc
     3481 gccgccagtt caaacagttg tgtttcgctg gccgcccgcg tagcattgac ggtagataag
     3541 atcccgccgc ccgctctggc gatatcttca taagccacgc cttgcaaccg catctcaaac
     3601 tcgttcgccc tctggccgcc atacaccaga tgtgtatgac aatccaccag accgggtgtc
     3661 agcaacttac cctgtagatc gtgttcctct gtaatatcag ccgtcagttc acggggggtc
     3721 tgcgtggcct tcccaagcca gacaatcgag tcaccacggc tgattaatgc accgttttca
     3781 atcacccccc aatcattagc cgaaagcgtc gtcagggtgg tatttcgcca gagcgtaaat
     3841 gcactcataa ggatttttcc cctcgtagat aataaatcca ccaacccgcc agctcgccgg
     3901 cggagacttt ttgccgtggt acgaaacgcc aggagttgcc atcagacgca aaccgccata
     3961 acaacgtatt cgccgttaca aacaccctgt tctcgccgct tatccactcc ccttcacaca
     4021 gcgtgaacag ccccgcttgc gcatgcgccg gagggagcca gtcaatgacg ctttgcacac
     4081 gacgcatccc cgcatcgcct tcccgcgcca taacattcag atcgcgaacc ccgccgccca
     4141 gcagctggca atcaggcata gcttctccag ggaaaattaa ggcgggatcg tgacgttgca
     4201 ggttgatgat cgaatcggca aacgttagct tcaccccttc cccgtcgaga atcgcgaggc
     4261 cacgaaccac gccaggccac gctgaaaaag ggccatcctg actgacattc gccatactga
     4321 tgcgcaacaa ccagttttca tcatcgggcc aggcaagcag ctgtcgggta aaccccccgc
     4381 cgttgcgcca cggttgcagc ggcttatcca ttgcagaaat cagcatcggt tccatattaa
     4441 gactcgggat aaatcgtata agcaggaacg caggtggtgc gtttggccca cgggtaatag
     4501 ataacggcgg taattaacaa gccgacaatc caggagatat ccgccccatc catcaggaca
     4561 gaaatgggtc cctgatacag cttttgattc aggaagggga tctgcaccag cacaccaatt
     4621 ccgtagcaca ccaacgcggt tttgttatac gcgccatagc gtccctccgg ggtataaagt
     4681 gccggaatat cggttttacc ttgtgagatg aagtagtagt cgatgagatt gatagcgctc
     4741 cacggcacaa ataccgtcaa cagcagcaga acgaagtttt tgaaattatt caaaaagtct
     4801 gcgcttgccg ccaacgcgac gaccatcgac aacatgacaa accccaccac gtaagcggcg
     4861 cggactcgtt gagaaacctg actgcgatta ttaaaggcgc tgacggtcgc cagaatcgac
     4921 ataaagccac cgtaggcatt caaacagttc accgtcagtt tgccgcaaac aatcaccgta
     4981 taaatcagaa tggcgaaggt gggtccggct aactcaccca taaaaccaac ctgatccttc
     5041 aggaagttag caccacaggc cgcaacaagc acgccaaacg tcatggcaca ttgcgcgcca
     5101 agcgtggttc ccagaaaggt ggcaaagaag attgattttg ctggcgtatc agagggtaaa
     5161 tagcgggaat aatcagaggc gtaaggggca aacgttaatt gccatccggc agagagagca
     5221 atcgttaata aaaagaccga cagactaaat ggcttttgac caaatgccgc agccacatcg
     5281 tagtgaccgc taagttgagt ggccaggtaa ataaacccca gaatacttaa tatccctgca
     5341 acacggccaa cgacgtgaat aagtttataa ccgaccaatg cgatcatcgc ggtcagcatg
     5401 ccgaaaataa taattccggt cgatgccgat tcgaaaccaa atatccggtt aatcgcctga
     5461 ccggataata ctgtacccgt cgcggcaaat ccaaggtaca taataatcac catcagtaac
     5521 ggtagcgccg ctcctttaac gccaaagcgg atacgactgg agatcatctg gggtaatccc
     5581 agctttgccc cctgcgacga gtgcaatgcc attactgcac caccaataat attaccgatt
     5641 aataaaccaa ttattgctga catggcttcg gagccaaaaa taatcgctaa cgcgccgtcc
     5701 accacagcgg taatttgtaa gttagcactt agccataaag taaactgact ggatggcgag
     5761 ccgtggcgag cagactcagg aacggcgtta atcgtatttg attctaatac tggcttttta
     5821 ctgcggatag ggctgcttga ttccatgaaa aagtctcctg cataatgaga ggtcattttt
     5881 tgaaacagca atagagggtc gggagatatt tctctcgacg atgtaaaaat attttttgat
     5941 aattattgtt atacaggcga attattctcc gcctgtatta gcgcaaatat taaatgcggc
     6001 gttaatattt agcgaatcat cggtaatttc agaccatagt ttttggcaca ttcaattgcc
     6061 gcttcataac ccgcatccgc atgacgcata accccggacg cacagtcatt caccagcaca
     6121 cgcgccagac gtttttcggc ttcttcgcta ccatcggcaa caataaccat cccggcgtgt
     6181 tgtgaatacc ccatcccgac gccgccgcca tgatgcaggg atacccacgt tgctccccct
     6241 gcagtgttga gcaaaccgtt gagcaacggc cagtcagaaa ccgcatcact gccatcaagc
     6301 atgctttcgg tttcgcggtt cgggctggcg acagaaccgg tatcaagatg atcgcgccca
     6361 atcacaatcg gtgcttttag ttcactgttg cgcaccattt cgttgaatgc cagccccgcc
     6421 aggtgacgct caccaagccc cagccagcaa atacgtgaag gaagtccctg gaaggcaatg
     6481 cgctcgcgcg ccatatccag ccagcgatgc gtcagtacgt tattggggaa caactcttta
     6541 attttggcat cagttttata gatgtcttcc gggtcgccag acagcgccac ccaacggaat
     6601 ggacctttgc cttcacaaaa cattggacga atataagccg gaacaaagcc cgggaaatca
     6661 aaggcatttt gcagaccttc gttgaaggcc acctggcgga tgttgttgcc ataatcgaca
     6721 accgggatcc ccatcgcctg gaagtccagc atcccctgaa catgtgcgac gcagctctgt
     6781 gccgcttctt tttgcaatct ggcgtgttgc tgtggatcct gctgtgcagc gcgccattgt
     6841 tctacgctcc agcccatcgg taggtagcca taaaccagat cgtgagcgga agtctggtcg
     6901 gtcaccagat ccggcttgat cgccccctga cgtgcacgtt tcaccagttc aggaacaatt
     6961 tccgccgcat tgcccaacag cgcgattgaa acagcgtctt tgcgctcagt atggtattta
     7021 atcagcgcca gtgcatcatc gagatctttc gcttgtttat cgacataacg ggtacgcaga
     7081 cggaagtcga tgctactttg ctggcactcg atatttaatg agcacgcccc cgccagagtc
     7141 gccgccagcg gttgtgcgcc acccatacca ccgagccccg cggtcaggat ccattttcca
     7201 ctcagcgaat tgttgtagtg ctgacgaccc gcttcaacaa aggtttcgaa cgttccctgc
     7261 acaatgccct gactaccgat gtagatccac gagccagcgg tcatctggcc gtacatgaac
     7321 agacctttac gatccagttc attgaaatga tcccagttgg cccattgcgg taccaggtta
     7381 gagttggcaa tcaatacgcg cggcgcatcg gtatgcgttt taaacacgcc tacgggttta
     7441 ccggactgaa ccagcagtga ctcatcagct tccagatttt tcagtgattc aaggatcttg
     7501 tcaaaacact cccagttacg tgccgcacga ccaataccac catacaccac cagatgctgt
     7561 ggattttcgg caacgtcggg atcaaggttg ttctgcaaca tccggtaggc tgcttcaatt
     7621 tgccagtttt tgcagtgcag ctgcgtaccg tgtggcgcat ggattacacg gctggtatca
     7681 atacgcgggt ctgaaggctt actcatgaat cttctcctgt tacttgataa aattaaacgc
     7741 tgtaatcggt cagaaaaaac gaacgaatga atatagaaac ggtt
//
LOCUS       DAZFJL010000177         7770 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFJL010000177 DAZFJL010000000
VERSION     DAZFJL010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.179"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(240..326)
                     /locus_tag="ACHJ8C_004225"
                     /pseudo
     CDS             complement(240..326)
                     /locus_tag="ACHJ8C_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450687.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            368..598
                     /locus_tag="ACHJ8C_004226"
                     /pseudo
     CDS             368..598
                     /locus_tag="ACHJ8C_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            650..1585
                     /gene="ttcA"
                     /locus_tag="ACHJ8C_004227"
     CDS             650..1585
                     /gene="ttcA"
                     /locus_tag="ACHJ8C_004227"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO5553991.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLETLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1714..3087)
                     /gene="dbpA"
                     /locus_tag="ACHJ8C_004228"
     CDS             complement(1714..3087)
                     /gene="dbpA"
                     /locus_tag="ACHJ8C_004228"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO5553992.1"
                     /translation="MTAFSTLNVLPSAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDVSLFQTQGLVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQSVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLKAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGFDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3117..3290)
                     /gene="ynaL"
                     /locus_tag="ACHJ8C_004229"
     CDS             complement(3117..3290)
                     /gene="ynaL"
                     /locus_tag="ACHJ8C_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO5553993.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(3565..4548)
                     /gene="zntB"
                     /locus_tag="ACHJ8C_004230"
     CDS             complement(3565..4548)
                     /gene="zntB"
                     /locus_tag="ACHJ8C_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO5553994.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(4562..4609)
                     /gene="ydaN"
                     /locus_tag="ACHJ8C_004231"
     CDS             complement(4562..4609)
                     /gene="ydaN"
                     /locus_tag="ACHJ8C_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO5553995.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            4803..6035
                     /gene="dgcM"
                     /locus_tag="ACHJ8C_004232"
     CDS             4803..6035
                     /gene="dgcM"
                     /locus_tag="ACHJ8C_004232"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO5553996.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSEARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRNEEETALSCRLVLRKLTEAEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVFLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(6056..6619)
                     /gene="smrA"
                     /locus_tag="ACHJ8C_004233"
     CDS             complement(6056..6619)
                     /gene="smrA"
                     /locus_tag="ACHJ8C_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO5553997.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6814..>7770)
                     /locus_tag="ACHJ8C_004234"
     CDS             complement(6814..>7770)
                     /locus_tag="ACHJ8C_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5553998.1"
                     /translation="KPDLSLLKVADYPSIILLAMTLGCLEYTLEEGARWGWLDDNTIL
                     LTSVLALVSFILFAARTLKISNPIMDLHAFKDKNFTLGCFFSFSGGVGIFSTVYLIPL
                     FLGQVRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMAGLGGFVFSMYLF
                     TPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLASGVFNLTRNLGG
                     AIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNRSGSDQTSEILAS
                     TKLLSQLMLREAQTMAFSDTFLLISGLLFIAFLLVPAMNKSS"
BASE COUNT         2119 a         1967 c         1767 g         1917 t
ORIGIN      
        1 ctccattcgg gtgtcatggc tctttcattt gaaatacatt catatccaat gtaatatttc
       61 catgcgcaaa tataaccacc aaccataccc atcatttatt acagagaatt attttattca
      121 ccgaaaagtt tcacaaaata tcccctgata gaaaactaaa aaacactgca taatatcgct
      181 attctggcac ttgcgaaaaa ttatattctg cacttgttgt agcgttctat ttctccgcct
      241 ggcaacgaat gcacaatgtc cgccacagtt tctgttctaa tttcttcctg tgttaccgca
      301 tcctgtacgg gtagcgcaac accagcatgc taagcaaggc tttcccatca gatttcacta
      361 cgactatttg ggagcgagat ggaatgcagc agtaaaacgt gctggtattc gccgccgtaa
      421 tccgtaccat acgcgacata cttttgcctg ctggctgttg acggcaggag cgaacccggc
      481 atttatagcc agccagatgg ggcatgaaac tgcgcagatg gtgtatgaaa tttacggtat
      541 gtggattgat gacatgaacg acgaacaggt agccatgttg aatgcgcggt tatcgtagtt
      601 gcaaagtttg ccctcaatat gccccattta gtaccagaga actgaaataa tgcaagaaaa
      661 tcaacaaatt acaaagaaag aacaatacaa cctgaacaaa ttacaaaaac gtctgcgtcg
      721 taacgtgggc gaagccattg ctgacttcaa tatgattgaa gaaggcgatc gcatcatggt
      781 ttgcctctcc gggggtaaag acagctatac catgctggag attctgcgca atttgcagca
      841 aagcgcgcca atcaattttt cactggtggc tgttaacctc gatcaaaagc aaccgggctt
      901 cccggaacac gttctgcccg agtatcttga aacgctgggc gttgagtaca agattgttga
      961 agagaacact tacggtatcg tgaaagagaa gattccggag ggcaaaacca cttgctcact
     1021 gtgttctcgt cttcgtcgcg gtatccttta tcgcaccgca acggaactgg gggcgacgaa
     1081 gatcgcgctg ggtcaccatc gtgacgatat cctgcaaacg ctgttcttaa atatgttcta
     1141 cggtggcaag atgaaaggta tgcctccgaa actgatgagc gatgatggca aacatatcgt
     1201 gattcgtccg ctggcctact gccgcgagaa agatattcag cgctttgccg atgcaaaagc
     1261 gttcccgatt attccgtgca acctgtgcgg ttctcagcct aacctgcaac gtcaggtaat
     1321 tgctgacatg ttgcgtgact gggataaacg ttatccgggg cgtatcgaga cgatgttcag
     1381 cgcaatgcag aatgtggtgc cgtcgcatct gtgcgatacc aacctgttcg atttcaaagg
     1441 cattacccac ggttctgaag tggttaacgg gggtgatctg gcgtttgatc gcgaagagat
     1501 cccactacaa ccagcgggct ggcagcctga agaagatgaa aatcagttgg atgagttacg
     1561 gctgaatgtg gttgaagtga aataacccgg atagcgcccg atgcgcaagc gtatcgggct
     1621 actcttatgg aggccggata agacgcggcc agcgtcacat ccggcaatgc cgaagaagat
     1681 gtttactctt gcaccctgca attcaacatt tcattatttt aataaccgca cccggcacgt
     1741 ttttccttta atcttcccac cctgtagctg tttccatgct ttatgagcaa cagcctgacg
     1801 caccgcaaca tagacatgcg ccggatgcac ggcaattttg ccaatatctg cgccatcaaa
     1861 cccgatgtct cctgtcagcg cacctaatac atcacccggg cgcattttgg cttttttccc
     1921 gccatcgata cacaacgttg ccatttctgc tttcagcggc acaatggaac tatttgctgg
     1981 cggcgtttgc cagttaagtt ttatctgcaa catgtcagaa atgatattgg cccgctgtgc
     2041 ttcttccggg gcacagaaac tgatcgccag accgctattt cctgcacgag ctgtacgacc
     2101 gatgcgatgt acatgaactt cagggtccca cgccagctca aagttcacca ccagctcaag
     2161 cgattttata tccagaccac gcgcagcaac atcagtcgcg accagtacac gggcgctacc
     2221 gttagcaaaa cgtaccaggg tctgatcgcg atcgcgttgc tccagatcgc cgtgtaacga
     2281 caatgcactt tgccctactt cattcagcgc gtcgcagaca gactggcaat cttttttggt
     2341 attgcaaaac accacgcaag aggatggctg atgcaagctt aataaccgtt gcaacagagg
     2401 aattttgcct ttgctggatg tctcataaaa ttgttgttca ataggtggca gagcatctgt
     2461 tgagtcaatt tcaatcgcta aaggatcgcg ttgcactctt ccgctgattg cggcgatggc
     2521 ttccggccag gttgccgaaa acagaagcgt ctgccgagat gctggcgcaa aacggatgac
     2581 atcatcaatg gcgtcgctaa atcccatatc cagcatgcgg tcggcctcat ccatcaccag
     2641 cgtattcaac gcatccagtg atacagtgcc tttttgcagg tgatccagca aacggcccgg
     2701 cgttgccacg ataatatgcg gcgcatgttg caacgaatca cgctgcatac cgaacggttg
     2761 accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc gacgcaattc
     2821 acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaaccct gggtttgaaa
     2881 tagcgacaca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt tgccgctgcc
     2941 ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg cggcggcctg
     3001 caccggcgtc atggttaaat aacccaactc attaaggttt gtgagttggg cggaaggcaa
     3061 aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac gcaaagtcag
     3121 caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg ttcttcatcg
     3181 ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggtactgg aacaggatcg
     3241 ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat atttccctcc
     3301 ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa aaaaagccga
     3361 ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa aaaaggaagt
     3421 aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca ggagagatat
     3481 tgccctgaat gggtagagag tttattgact tcgctcaaac tttacggcgt ttttgtatac
     3541 agacagccgg aaaaattgct tttgttacaa ccatttacta cgatgcaacc ataaagcaac
     3601 accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt gccatccacc
     3661 gccagggatc ccaccaaggt tgacgccaaa taaccctgtc agaaacgtac tgggtaaaaa
     3721 gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct gcatcacctg
     3781 agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt cgtcaagacc
     3841 gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc tcatccacgg
     3901 caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat agcgacgcat
     3961 cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt gctgatcgag
     4021 aagattatct tcgaggtcga taattttatc gtgcagctgc tcgataaatt cgctggaatg
     4081 atcggtcaac gcatcgcaca catcaaccag ccaccccccg caatcggtcg gaccagtgcc
     4141 ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc gggtcgaaac
     4201 aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc gttcatcggt
     4261 gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgagacggc tgactcgggg
     4321 ccgtgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg gtgttgtcgc
     4381 cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg gatgcgcttc
     4441 atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca gcatccaggc
     4501 aaatactgca tccgggacat taacgtccga tcccttaatc gcttccacag tgcctccatc
     4561 atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc tcatcacccc
     4621 attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc aaaatgcttt
     4681 tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa ttatctgatc
     4741 atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat aatgggatct
     4801 caatgattac acacaacttc aataccctgg acttactcac cagtcctgtc tggatcgttt
     4861 cgccctttga ggaacagtta atttatgcca acagcgaggc gcgactgttg atgcaagacc
     4921 tcacgtttag tcagctacga accggtccct attccgtctc ctcacaaaaa gaactgccga
     4981 aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcacc gttcagcgca
     5041 atgaagagga aacagcattg agctgtcgac ttgttttgcg aaagctgaca gaagcagaac
     5101 cggtgattat tttcgaaggt atcgaagcgc cggcaacact gggcttaaaa gccagtcgct
     5161 cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg tttttttctg actaactctg
     5221 caccaatgtt gttgattgac ccctcacgag atggccaaat cgtcgatgct aacctcgccg
     5281 cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc tgggaaatta
     5341 atatgctcgg gcgtcgcgtc atgcctatca tgcatgaaat ctcgcattta cccggtggtc
     5401 ataaaccttt gaattttgtt cataaactgg cggatggttc gactcgtcat gtgcagacct
     5461 atgccgggcc gattgaaatt tatggcgaca agctcatgtt atgtattgtg catgatatta
     5521 ctgagcaaaa acggctggag gagcagctgg aacatgctgc ccaccatgat gcgatgaccg
     5581 gattactgaa tcggcgacag ttttatcaca ttaccgagcc aggccaaatg cagcacctcg
     5641 ccatcgctca ggattacagc ttgttgctca tcgacaccga tcgttttaaa cacattaacg
     5701 atctctatgg gcattctaaa ggtgatgagg tgttatgcgc ccttgcccgc actctcgaaa
     5761 gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt gtctttttgc
     5821 taccaagaac cccactggat accgcgcttt cgctggctga aactatccgc gtaagcgtgg
     5881 caaaagtgag tatttcgggc ttaccacgct ttactgtcag cattggtgtg gcgcatcacg
     5941 aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg tatcgagcga
     6001 aaaatgatgg acgcaaccgc gtgctggcgg cataagccac ggatgcgtct cgtgatcaac
     6061 gactgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgctgtt ttacgtagtg
     6121 cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc agtgcaatat gcctgaacat
     6181 catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca tgcgatttat
     6241 catcccgtcc tttgccatga ataatcagca cgttacgcaa accatccgcc agggcttgtt
     6301 gaatgaaact gaacatcatt ttgcggcact cttccaccgg ctggcgcaaa agattcaggc
     6361 tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgt tgcaaacctt
     6421 cccgccgaaa ctccagcggc tgacttagcg ggatgatgtc gagaaatcca gtggtgagga
     6481 aattatcaag ttgcagcgtg tcgatacgct gcggcgcacg ttggttacgc gttggatgcc
     6541 agtggacatc tgtagcacgt ttcagcggct ggacatcttc catggcgtca agaaacagcg
     6601 atttgtcgtc aaggttcatg taacctccta tgcaccttca cacgtcgtga ttcagacggg
     6661 ttaattgttc atacttactg catcgttccc ttcacagatt gaaacaatac tctacttcat
     6721 acctgtgagt atctgcgcag gcggggcatt ttatactagc ctgttagcag tatcgaacag
     6781 tttagcccga gtttaattca gcacaggctc cgatcaggat gatttattca tagcaggaac
     6841 caacaaaaat gcgataaata acagaccact gattagcaga aaggtatccg aaaatgccat
     6901 agtttgcgcc tccctcagca tcaattgact caataatttg gttgaagcca gtatttccga
     6961 tgtctggtca cttccactgc gattaaaaaa caaagcactt ctggaaatga agtcattcac
     7021 cgtatgggga acagaaacca ttttttcacc cattctcgaa aaatgaaaat tagttcgatt
     7081 attgagaata gagccacaca gggcaatacc aatcgcgccc ccaagattac gtgttaaatt
     7141 aaatacacca gaggctaact tcagccgttc ttttggaatt ccccccagcg ttaatgttac
     7201 gatgggggcc atcgcaaact gctgcgaaat ccctctaatt gcctgtggaa acagtaactc
     7261 ctgccatccc cattcgtggg ttataggggt aaaaagatac attgaaaaca caaatccacc
     7321 cagccccgcc atcaacagcc atcgcagatt tattttttta cttaaccaga agtaaaaggg
     7381 aacagaaaat aactggaaaa tacctgttgt gcaaacggca aaacctatct cttcggcatt
     7441 taatccgcga acctgcccca aaaacaaagg tattaaatat acagtagaga atattccaac
     7501 ccctccagaa aaagaaaaga aacatcccag tgtgaaattt ttatccttaa aagcatgaag
     7561 atccattatc ggattggata ttttaagtgt acgcgcagca aataaaataa aacttaccaa
     7621 tgccagaact gatgtcagca aaatagtatt atcatccagc catccccagc gcgctccttc
     7681 ttcaagggta tattccagac atcccagtgt catggccaac aggataatac tcggataatc
     7741 agcaactttc agtaaagaga gatcaggttt
//
LOCUS       DAZFJL010000178         7565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZFJL010000178 DAZFJL010000000
VERSION     DAZFJL010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.180"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            127..450
                     /locus_tag="ACHJ8C_004235"
     CDS             127..450
                     /locus_tag="ACHJ8C_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO5553999.1"
                     /translation="MRLLILTLSLITLTGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HARTDRYVSRGIADRACQQAGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            622..1119
                     /gene="ftnA"
                     /locus_tag="ACHJ8C_004236"
     CDS             622..1119
                     /gene="ftnA"
                     /locus_tag="ACHJ8C_004236"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO5554000.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(1157..1396)
                     /locus_tag="ACHJ8C_004237"
     CDS             complement(1157..1396)
                     /locus_tag="ACHJ8C_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO5554001.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTQANLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            1587..2798
                     /gene="tyrP"
                     /locus_tag="ACHJ8C_004238"
     CDS             1587..2798
                     /gene="tyrP"
                     /locus_tag="ACHJ8C_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO5554002.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQLATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2860..3525)
                     /gene="yecA"
                     /locus_tag="ACHJ8C_004239"
     CDS             complement(2860..3525)
                     /gene="yecA"
                     /locus_tag="ACHJ8C_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO5554003.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALDAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3721..3807)
                     /locus_tag="ACHJ8C_004240"
     tRNA            complement(3721..3807)
                     /locus_tag="ACHJ8C_004240"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3771..3773),aa:Leu,seq:taa)
     gene            complement(3820..3893)
                     /locus_tag="ACHJ8C_004241"
     tRNA            complement(3820..3893)
                     /locus_tag="ACHJ8C_004241"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3859..3861),aa:Cys,seq:gca)
     gene            complement(3947..4022)
                     /locus_tag="ACHJ8C_004242"
     tRNA            complement(3947..4022)
                     /locus_tag="ACHJ8C_004242"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3987..3989),aa:Gly,seq:gcc)
     gene            complement(4174..4722)
                     /gene="pgsA"
                     /locus_tag="ACHJ8C_004243"
     CDS             complement(4174..4722)
                     /gene="pgsA"
                     /locus_tag="ACHJ8C_004243"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO5554004.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4779..6611)
                     /gene="uvrC"
                     /locus_tag="ACHJ8C_004244"
     CDS             complement(4779..6611)
                     /gene="uvrC"
                     /locus_tag="ACHJ8C_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO5554005.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6608..7264)
                     /gene="uvrY"
                     /locus_tag="ACHJ8C_004245"
     CDS             complement(6608..7264)
                     /gene="uvrY"
                     /locus_tag="ACHJ8C_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO5554006.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
BASE COUNT         1837 a         1954 c         1769 g         2005 t
ORIGIN      
        1 gcagcccccc caatttctga cacatataga tggcaagaaa tagcgcctgc caggcgtctt
       61 ttccggccat tgtcgcagcg ctgtaacgcg taaaatactg ctttctttta ctcttctggc
      121 tggaccatga gacttctgat tctgactctt tccttaataa cgctgacagg atgtacggtt
      181 actcgccagg cccatgtgag cgaagttgac gcggcaacag gtattgttcg gttggtttat
      241 gatcaggctt ttttgcagca tgcccgtacc gatcgttatg tcagtcgcgg gatcgccgat
      301 cgcgcatgcc agcaggcagg ctatacccac gctatcccct ttggtcagcc agtaggcaac
      361 tgtagcctct ttgcgggttc cttatgtttg aatacggaat tcactttgtc atatcagtgc
      421 catcattccg ccttccctgt ctttctctaa acgttttaat cagcgagggg gatcttcgct
      481 gattaaataa atagcggtaa tgcgttttat tcccattcgt atttttaata aatgatgttt
      541 atattttacc ttttgcaaat aataaaataa caaattatag tgacgctaca gcaacaaata
      601 taacctttgt ggagcactat catgctgaaa ccagaaatga ttgaaaaact taatgagcag
      661 atgaacctgg aactgtactc ttcactgctt tatcagcaaa tgagcgcctg gtgcagctat
      721 cataccttcg aaggtgctgc cgcgttcctg cgccgtcacg cccaggaaga gatgacgcat
      781 atgcagcgtc tgtttgatta cctgactgat accggcaact taccgcgtat taataccgtt
      841 gaatctccgt ttgctgaata ttcctcactt gatgaattat tccaggaaac ctataaacac
      901 gaacaattaa tcacgcagaa aattaacgaa ctggctcatg ctgcaatgac caatcaggac
      961 tacccaacat ttaatttcct gcaatggtat gtttctgagc agcatgaaga agagaaactg
     1021 ttcaaatcga ttattgataa attaagcctt gcaggcaaaa gcggcgaagg tctgtatttt
     1081 atcgacaaag aactctctac cctcgacaca caaaactaat gctctccggc ggcaggtttt
     1141 ctgccgccgt ttcaaatcag tgacggcaaa tcttactctg atcggttgaa aaaccttctt
     1201 ctgaaggtat aaatttgcct tttgctgcca gaaacgccac caactctccc gcagtcatcc
     1261 cttctgccga gcaggtgtga aaacgtgcct gttcaccaaa acgcgcttta attgctgctt
     1321 ccagattggc ttgcgtatat tgctcgcctg attcaatcat catatttaac acttcatgac
     1381 cgtgaataga gtccatcgtt cctcctcaaa aaaagcctag cgtagcgatt gccgcttatg
     1441 aagactttgc gccagcgcag aactgaacgc tttttattgt acatttatat ttacatcata
     1501 tgtaacgccg gtttgacgaa tcagccgtta tgccttaacc tgcgccgcag atatcactca
     1561 taaagatcgt caggacagaa gaaagcgtga aaaacagaac tctgggaagt gtttttatcg
     1621 tggcgggaac cacaattggc gcaggcatgc tggcaatgcc gctggctgcg gcaggtgttg
     1681 gttttagcgt cacgttaatc ttgttgattg ggctttgggc gttgatgtgc tacacggcgc
     1741 tattactgct ggaggtgtac cagcatgtcc cggcagatac cggtctgggc acgctggcaa
     1801 aacgctatct gggacgctac ggtcagtggc tgacgggctt cagtatgatg ttcttaatgt
     1861 atgctctgac tgcggcatac atcagcggtg ccggtgaatt gttggcctcc agcatcagcg
     1921 actggacagg tatttcaatg tcggcaaccg ctggcgtgct gttgttcact tttgttgccg
     1981 gtggcgtggt ttgtgtcgga acttcgctgg tcgatttatt taaccgtttt ctgttcagcg
     2041 ccaaaattat ttttctggtg gtgatgctgg tattgctgct accgcatatt cacaaagtga
     2101 atcttttaac cctgccgttg caacagggac tggcactgtc tgcaatcccg gtgattttta
     2161 cgtcgtttgg ttttcacggt agcgtgccga gtattgtcag ctatatggat ggcaacattc
     2221 gtaagctacg ctgggtgttt ataatcggta gtgcgatccc cctggtggca tatattttct
     2281 ggcagctggc gacccttggt agcattgatt caacaacctt tatgggattg ctggctaatc
     2341 atgccggatt aaacgggctg ttacaggcgt tacgcgaaat ggtggcctct ccgcatgttg
     2401 agctggcagt gcatttattt gctgatttag ccctcgccac gtccttcctc ggcgtagcgt
     2461 taggcttatt tgattatctg gcagatttgt ttcagcgttc aaataccgtt ggtggacgat
     2521 tgcaaactgg tgcaattacc tttctgccgc cactggcgtt tgcactgttt tatccacgag
     2581 gatttgtgat ggcgctgggt tacgccggtg tggcactggc ggtactggca ttgattatcc
     2641 cttcgctgtt gacctggcaa agcaggaagc acaatcctca ggcgggttac cgggtgaaag
     2701 gtggtcgtcc ggcgctggtg gtggtgtttc tctgtggtat tgctgtgatt ggcgtgcaat
     2761 ttttgattgc ggcaggcttg ttaccagaag tggggtgatt agatagcctc aaattcctta
     2821 ttgggtgcca gaattaacgc tgacacccaa tttgacctct taatgcaggc agcactgctt
     2881 aaatttctta ccactaccgc acggacaagg atcgttacgc cccggtttct cttctgcttt
     2941 gataggttgc tgaacagctt tttcctgcgg atgcgccatc cagtatgcat gtaaatcaag
     3001 cgccgccagt cgaatagcat ctacgctctc ttcaaacgct tctggcgaca tcttctctac
     3061 ccgctcgaag ttttcctcag taccgtgcag cgcaatcgca tccagcgctg gttttaacga
     3121 atcgggcaac gttgaccagt cagaaagtgc cacgccccgc atatagccaa agcaccactc
     3181 ctcaacaatc gtcagctcgc tgccatcgac ttctcgcaag ccgaataacg gctcaaactg
     3241 ctccggaaat tcgttcagac gctctgcggt atcggccata tgttgaaaag ccaggttcat
     3301 aaagcgcgtc atctctttct ctgacgccca gcgcggcaca tagtcagccc caccccacac
     3361 ggcaaccagc cactgtgccg gttcaatctc ttgcggagaa ctcaacaccg ccgtcaataa
     3421 accgtccagc tccgccacat caagaatggc gtggtcagtg ttgtatttgg tcagaatatc
     3481 gtccagccat tccaactcac tttcgtttaa cggtcccgtt ttcatacgct tttccttgtg
     3541 gatctcaact cgccagcacc tatcttacat gccggtccgt atcagagata ctttttgagt
     3601 ggctttgccg gtgattaaaa attaaggagg gtgtaacgac aagttgcagg cacaaaaaaa
     3661 ccacccgaag gtggtttcac gacactgctt attgctttga ttttattctt atctttccca
     3721 tggtacccgg agcgggactt gaacccgcac agcgcgaacg ccgagggatt ttaaatccct
     3781 tgtgtctacc gattccacca tccgggctcg ggaagaaagt ggaggcgcgt tccggagtcg
     3841 aaccggacta gacggatttg caatccgcta cataaccgct ttgttaacgc gccaaattct
     3901 tcaggccttt cagccagaca tccgctgacg ccgatgcctt ttaaactgga gcgggaaacg
     3961 agactcgaac tcgcgacccc gaccttggca aggtcgtgct ctaccaactg agctattccc
     4021 gcattcatca agcaatcagt taatcacttg attttattat cgtctggcaa tcagtgccgc
     4081 cgttcgatgc gttgcattct acttacctgg cgcgatgagt caacgatatt tttcaccact
     4141 tttgatcgtt tgctgaaaat tgcgccgaaa cgatcactga tcaagcaaat ctgcacgcgc
     4201 agcgctcaaa tattgcaaca ttgaccacag agtcagtacc gcagccacaa agaaaagtgc
     4261 aataccggcg tactcaaccc aaatgttcgg acgccacagc agccaggcta acgccaccat
     4321 ctgggcagtg gttttcactt tcccaatcca ggagacagcc acgctactgc gtttacccaa
     4381 ctccgccatc cattcgcgta gcgcagaaat aataatttca cgggcgatca tcgttgctgc
     4441 cggtaaggtc acccaccagc tgtggtaatg ctcggttacc agcaccatgg cgatagccac
     4501 gagaacttta tctgcgacag ggtcaaggaa agcaccaaac cgggtactct ggttccagcg
     4561 acgtgccaga aaaccatcga accagtcagt caccgccgcg acgcagaaaa tgagcgcggc
     4621 ggcaaacggc gaccaggtga caggcagata aaagaccaat acaaagaatg ggataaggat
     4681 gacacggaac agtgtaagca acgtagggat attaaattgc ataatgacgg gtaactatct
     4741 gttgtcagta agattacccc tatgttgcta cagagacatc aatgtttcaa cgaccagaag
     4801 atcttttctg ccagaccttg cgaaataccc ggcacttttg caatttcctc gacgctggcg
     4861 ttacgtaaac cttgcaaacc acccatatat ttcaacaaca tttgccgacg ttttggcccg
     4921 acgccttcaa tggtttccag ggaactggta tttttgacct tcgcccgttt tttacggtgc
     4981 ccgccaatcg cgtgatcgtg tgattcatcg cgaatatgct ggataacatg cagcgcggga
     5041 gaatctggcg gcaaactaaa tccctcacct tccggctcaa agaacagcgt ttccagccca
     5101 gccttacgat ctgctccttt ggcaacgcca agtagcagcg gatgattttt atcccatgag
     5161 acatccagtt cggcgaagac atttttcgcc tgcgcaagct ggcctttgcc gccgtcgata
     5221 aggatcacat ccgggatctt actgtcgtca atggctttac cataacgccg acgcagcacc
     5281 tgattcatcg ccgcataatc atcgcccggc gtgatgccag taatgttata gcgccgatac
     5341 tccgcacgca gcgggccgtt agcatcaaac accacacagg aagcgacggt ttgttcaccc
     5401 atggtatggc tgatgtcaaa gcactccatc cgcttcactt ccggcaattt caacacgctg
     5461 gcaagcgcgg tcagccgctg gtgaacggta gattgctgcg aaagtttgct ggttaaggcc
     5521 gtcgccgcat tggtacgcgc gagtttcaga taacgcgctc tatcgccacg aggtttggtt
     5581 tgaacattaa tcttgcgtcc cgccagttct gaaagggaat cggcgagcag cgttttatcg
     5641 ctaagattaa aatcgagcag gatctcaccc ggtaaggtgc gcatctggct gccttgtaaa
     5701 tagaactgac ctacgaaggt ttccaccacc tcgctcagtt ccgtaccgcc aggcactttc
     5761 gggaaatagc tgcggctgcc gagcactttg ccctgacgaa tgaacaatac gtggacacaa
     5821 gccatacccg catcgaacgc cactccaata acgtcaaggt cgtcgccggt attggaaaca
     5881 aactgttttt cggtgacacg tcgcaccgcc tgaatttggt cgcgaatccg cgccgcttct
     5941 tcaaactcca gattctggct ggcagtttcc atacgactaa tgagttgcgt aagcacctga
     6001 tcatctttgc cagacaaaaa caggcgcaca tactcgacct gctgagcgta ttcttcttca
     6061 ctcaccagtc cttcaacgca cggccccaga cagcgcccta tctgatattg cagacacgga
     6121 cgcgagcggt tgcgataaac actgttttcg cactggcgaa tgggaaaaat cttttgcagt
     6181 agcgccagtg tttcacgtac ggcatagcca ttcgggaacg ggccgaaata ttcacctttg
     6241 gcatgcttcg ctccacgatg catcgccaga cgcgggtggg tatcgccact caggaagata
     6301 aaaggatatg atttatcatc gcgtagcaaa acgttgtaac gcggctgata aagtttgatg
     6361 tagttgtgtt ccagtaatag cgcttcggtt tctgtatgag taaccgttac atcaatttgc
     6421 tggatctggg cgaccagcgc ttcggttttg cgcgaagcga ggttgctacg gaaatagctg
     6481 gaaagccgtt ttttcaggtc tttcgctttg ccgacataga taaccgtacc accagcatcg
     6541 tacatgcgat aaacgcctgg ctggctggtt acggttttta aaaacgcttt tgcgtcaaac
     6601 tgatcactca ctgacttgat aatgtctccg cattacacag accatggcga attgccaggt
     6661 gagtcagctc aacatcgcca tgaatgttta gtttactgaa catacgatag cggtagctgt
     6721 tcaccgtttt cggactgaga ttgagctgtt ctgagatctc attgaccttc tggcccttgg
     6781 tgatcatcag cataatctgc aattcacgtt cagacaaact ggcaaatggg ctttctgttt
     6841 tttctggttc gatctggctt aacgccattt gttgagcgat atcagaagca atgtaacgct
     6901 gccctgaata gacagaacga atcgcactca cgacttcctg cggagccgcg cctttgctga
     6961 ggtagcccgc agctccggcc tgcatgactt tcgctggtaa agggttttct gtatggacgg
     7021 taagcatgat gattttgaca tcagctgtgg aacgcgcgat tttacgcgtc gcctcaagac
     7081 cgccaatgcc cggcatactc atgtccatta gcaccacgtc aacggcattt gcccggcacc
     7141 atttaacggc gtcttcaccg cacgatgcct caccgacgac ttttataccc tttatatctt
     7201 ccagaatgcg tcgtatccct gcgcgcacca gttcgtggtc atcaacaagt agaacgttga
     7261 tcaaaggaat atctccagaa atagggataa cgctactgat agttagtcat tcgtatatta
     7321 gcggttttta ttgcaacttt gaaacgttaa aaatggtggg tattcgattt ttctctcgtt
     7381 tttgtcaatt cgattgtcca cagttatggt cacgcccgtc accaacggct tccgggatcg
     7441 tttcacagta agcccggcga tgaaatgtga caaaaattga ctttattcag caaaaatgaa
     7501 aatcagcccg ctgaatatgt aacattaatt aacccgaaca atacgggcaa aaaacattca
     7561 tgata
//
LOCUS       DAZFJL010000179         7369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZFJL010000179 DAZFJL010000000
VERSION     DAZFJL010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.181"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            146..736
                     /locus_tag="ACHJ8C_004246"
     CDS             146..736
                     /locus_tag="ACHJ8C_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5554007.1"
                     /translation="MSKKLGFALSGIMLAMAAGTAFAANDMDGGQLNISGLVVDNTCE
                     ARVDGGNKDGLILLQTATVAEITDGVLDTTVGAKAKPFSITIDCSKANPAPGTTAKMT
                     FGSVFFGNSKGTLNNDMSINTPSDGVNIALHNIEGSTIKQVQVNNPGDVYSKTLDSTS
                     KSATYDFKASYVRADASKAATAGYVKTNSAYTITYQ"
     gene            845..1585
                     /locus_tag="ACHJ8C_004247"
     CDS             845..1585
                     /locus_tag="ACHJ8C_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO5554008.1"
                     /translation="MFFNTKHTAALCLASCMAFSSSAIADIVISGTRVVYKSEQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASS
                     ALPKDRESVFWFNVLEVPPKPDAAKAANQSLLQLAFRTRIKLFYRPEGLSGISSDAPL
                     ALKWSWATSEGKAALRVDNPTPYYVSFSSGDLEANGKRYPLDMTMIAPFSNDVIKVNG
                     MSGRTSSAKVHFFAINDFGGSIEGNANL"
     gene            1620..4208
                     /locus_tag="ACHJ8C_004248"
     CDS             1620..4208
                     /locus_tag="ACHJ8C_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFO5554009.1"
                     /translation="MTIKSTNHLTHIATFCALLYSNSALCAELVEYDHTFLMGKDASN
                     IDLSRYTEGNPTLPGIYDVSVYVNDQPIMSQSIAFTVIEGKKNAQACITQKNLLQFHI
                     SSPDKNSEKAILLKRDDDLGDCLNLAEMIPQSSIRYDVNDQRLDIDVPQAWIMKNYQN
                     YVDPSLWENGINAAMLSYNLNGYHSESPGRTNDSIYAAFNGGINLGAWRLRASGNYNW
                     MTNVHSDYDFQNRYLQRDLASLRSQLVIGESYTTGETFDSVSIRGIRLYSDSRMLPPV
                     LASFAPIIHGVANTNAKVTVMQNGYKIYETTVPPGAFAIDDLSPSGYGSDLIVTIEEA
                     DGTKRTFSQPFSSVVQMLRPGVGRWDISAGQVLKDSIQDEPNLFQASYYYGLNNYLTG
                     YTGIQLTDNNYTAGLLGLGMNTPVGAFSVDVTHSNVSIPDDKTYQGQSYRISWNKLFE
                     NTSTSLNIAAYRYSTQHYLGLNDALTLIDEVEHPEQDLEPKSMRNYSRMKNQVTVSIN
                     QPLKFEKKDYGSFYLSGSWSDYWASGQNSTNYSIGYSNSASWGSYSISAQRSLNEDGQ
                     TDDSIYLSFTIPIENLLGTEHRSSGFQSIDTQLNSDFKGNNQLNISSSGYSDTNRISY
                     SVNTGYMMNKSSDDLSYIGGYASYESPWGTLSGSASASSDNSRQFSLNTDGGFVLHSG
                     GLTFSNDSFSDSDTLAVIQAPGAKGARINYGNSTVDRWGYGVTSALSPYHENRIALDI
                     NDLENDVELKSTSTVAVPRQGAVVFADFETVQGQSAIMNIVRSDGKNIPFAADIYDEQ
                     NNIIGNVGQGGQAFVRGIGQEGNIRITWIEEGKPVSCFAHYQQNTTSEKIAQSIILNG
                     LRCQIQ"
     gene            4225..4797
                     /locus_tag="ACHJ8C_004249"
     CDS             4225..4797
                     /locus_tag="ACHJ8C_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5554010.1"
                     /translation="MIKTTPHKIAILMGLLLSPSVFATDVNVDFTATVKATTCNITLT
                     SLNGSNVVDNGNDSYTLIIPKMGLDKIVNKASQSEANFKLVASGCSSGISWIDTTMTG
                     NQSGSTSLIIPQSSDSSSTTSNIGMGFKRLETSGDTFLKPNSAEYIRWKTTEISTSGL
                     EMTVALRETTPGQGIPGKFRAQATFNFIYQ"
     gene            4809..5417
                     /gene="yadL"
                     /locus_tag="ACHJ8C_004250"
     CDS             4809..5417
                     /gene="yadL"
                     /locus_tag="ACHJ8C_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFO5554011.1"
                     /translation="MMILKKLCYGLSGSLFLASSLLTISSHAATDSIGLTVTTDIEMG
                     TCTSTLTDDGTAKLSTINFGDVYISEINAETKIKTFKLQFKDCAGIPGKKAKIKLTPR
                     ALCEGNSNNGPGFANASTATAKAAAVAVEVWSTSTPGKNGAKQFSCVTPATEEVSIAG
                     ATGGDVVDYPMSAVLVVAKDKTVTDVTAGDFTAPATFTVTYN"
     gene            5444..6040
                     /locus_tag="ACHJ8C_004251"
     CDS             5444..6040
                     /locus_tag="ACHJ8C_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO5554012.1"
                     /translation="MRPTQRNLMKKSILLLSLLFSTTFPVVAGQDVDIVANIKNNTCQ
                     SGISNNGNIDLGVVGVGDFTGNISAENYHPGGKEFTITVQDCTLQGTGNVLNQLHIDF
                     RALSGVMATGSSQIFANEDNTGAKNVGVVIFSIQDPANTFNVLGVTGSSRSVYPVMSS
                     ALNNSSWKFSTRMQKIDPALNVTSGQLISHVLVDIYYE"
     gene            6066..7355
                     /locus_tag="ACHJ8C_004252"
     CDS             6066..7355
                     /locus_tag="ACHJ8C_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFO5554013.1"
                     /translation="MQHDNGVKMKIFFRYFLFLALCSCCYTASVGAANNVSYVVGNNA
                     GVGPSDQKWKETGPNGDVTVKFRYGSGTNNLVFYKPTQLGPTGVSLKWAQLDSASGGG
                     FLYCNRSGSTSGGPMHIENKMVDSGKSYGGHKLFKTSVPGLYYTLAISNIWSTYTYTD
                     INPSGMYIGDSTSQSFNWRGESEQTLYWSCNNANTKNKYWAVGGVMQTLTIEFYTDTD
                     FNPTTNQRVTLSRTDNYLYSFKAYGAGGGINEHSYFLKIDFDLTDIVLTNPTCFTAAL
                     SGSSVSGSTVKMGDYTPAQIKNGATAVPFDITLQNCIRVRNIETKLKSNKVGSVSKEL
                     LANTLTGNDAAKGVGVLIEGLKNTKSAQMVLKPNDATSIYKDYETEDDTTGGIFPDQG
                     NGTSQPLHFQATLKQDGNIAIEPGDFKATSTFQVTYP"
BASE COUNT         2235 a         1603 c         1589 g         1942 t
ORIGIN      
        1 tcccaatgat gaatgaatgt aatacttagt aaaaaacaaa caaacatcat taacaaagta
       61 acgttcagtt acatggcatt gcatcaaaat ctcatcatca tgcaatgctg ttatctgctt
      121 tatggaatat tactcaagga tttttatgtc taaaaaatta ggttttgcgc tgtccggtat
      181 tatgttggca atggctgctg gtactgcatt tgctgctaac gatatggacg gcggtcaatt
      241 aaatattagc ggtctggtcg ttgataacac ttgtgaagct cgtgttgatg gcggtaacaa
      301 agacggtctg atcctgctgc aaaccgctac tgttgctgaa attactgatg gtgtactgga
      361 taccactgta ggcgctaaag ctaaaccatt cagcattact attgactgca gcaaagctaa
      421 cccggctccg ggtactactg caaaaatgac cttcggttct gttttctttg gtaacagcaa
      481 aggaacactg aacaacgaca tgtctattaa caccccgtct gacggcgtaa atatcgctct
      541 gcacaatatt gaaggttcta ctattaaaca ggttcaggtc aataatccgg gtgatgttta
      601 cagcaaaact ctggattcaa cgtctaaatc tgcaacctac gattttaaag catcctatgt
      661 tcgtgcggat gcaagcaaag cagctactgc cggttatgta aaaaccaact cagcatatac
      721 cattacttac cagtaaatat caaccgctta aattgatcgg gtgaatatat acagggaata
      781 acatcaaata tttttacttt tgataattat tccctgtttt aattaattct gtaaggaatg
      841 gtttatgttt tttaatacta aacataccgc agcattgtgc ttagcgagct gtatggcttt
      901 tagctcatca gctattgcag acatcgtaat ttcaggtaca cgcgtagttt ataaaagcga
      961 acaaaaaagc gtcaatgtac gtctggaaaa taaaggcaat aacccattgc tggttcagag
     1021 ttggttagat actggagatg ataacgctga accaggcagt attactgtcc cctttaccgc
     1081 tacgccgcca gtatcacgta ttgatgctaa acgtggccaa acaatcaaac tgatgtacac
     1141 cgccagcagt gctctgccaa aagacagaga aagtgtgttc tggtttaacg tactggaagt
     1201 acctccaaaa cccgatgcag caaaagcagc aaatcaaagc ctgctacaac tggcatttcg
     1261 cacacgtata aaactgttct atcgcccgga aggattgagt ggaatttcat ctgatgcacc
     1321 attggctctg aagtggtcct gggcaacatc tgaaggcaaa gcagcattac gcgttgataa
     1381 ccctacgccc tactatgtct cttttagcag tggtgattta gaagccaacg gcaaacgcta
     1441 tcccctggat atgacaatga ttgcgccgtt cagtaacgac gtcatcaagg taaatgggat
     1501 gagtggcaga acaagctctg caaaagtgca ttttttcgcc attaatgatt ttggtggatc
     1561 tattgaaggc aacgccaacc tttaatctgg caaggatata acacagcagg aagcagaacg
     1621 tgactattaa atctactaat caccttaccc acatcgccac gttttgcgct ctgctgtata
     1681 gcaatagtgc tttatgcgct gaactcgtcg aatatgacca tacatttctg atgggaaagg
     1741 atgcttcaaa tatcgacctt agtcggtata cagaaggcaa tcccacacta cctggcattt
     1801 acgatgtcag tgtgtatgtt aacgatcaac ccatcatgag ccaaagcatt gcttttactg
     1861 ttattgaagg taaaaagaac gctcaggcct gcattacgca gaaaaattta ttgcaatttc
     1921 atatcagctc accagacaag aatagcgaaa aagccatttt gcttaagcga gacgacgatc
     1981 ttggtgattg cctcaatctg gctgaaatga tccctcaatc atccattcgt tacgatgtta
     2041 acgatcaacg cctggatatc gacgtccccc aggcatggat tatgaagaat tatcaaaact
     2101 atgttgatcc ctctttgtgg gaaaatggca ttaatgcagc catgctttcc tacaacctca
     2161 acggatatca cagtgaaagt ccaggcagaa caaatgacag tatctacgcg gcattcaacg
     2221 gtgggataaa cttaggtgcc tggcggttac gtgcctcggg taactacaac tggatgacca
     2281 atgtccatag cgattacgat tttcagaatc gctatttaca acgcgatctt gcgtcactac
     2341 gttcacaact ggttattggt gaatcttaca cgaccggcga gaccttcgat tcagtcagca
     2401 ttcgcggtat tcgcttatac agtgacagtc gaatgctgcc tccggtatta gccagttttg
     2461 cgccaatcat tcatggcgta gcgaatacca atgccaaagt aaccgttatg caaaatggtt
     2521 ataagatcta tgaaacgacg gtgccgccag gggcttttgc cattgacgat cttagcccgt
     2581 caggttatgg cagtgacctc attgtgacta tcgaagaagc tgatggcaca aaacggacgt
     2641 tctcccagcc attttcatct gttgtgcaaa tgctgcgccc tggcgttggt cgctgggata
     2701 ttagcgccgg tcaggtttta aaagacagta tccaggatga gcctaattta tttcaagcca
     2761 gctactacta tggtttgaat aactacctga caggatatac cggtattcag ctaactgata
     2821 ataactatac cgccggattg ctggggcttg gtatgaatac ccccgtgggg gctttttctg
     2881 tcgatgttac tcactcaaat gtcagcattc cggacgataa aacctatcaa ggacaaagct
     2941 accgtatttc ctggaacaag ttatttgaaa ataccagtac atcattaaat atcgcggcct
     3001 atcgctactc cacccagcat tatctgggcc tcaatgatgc gctgacgctg attgatgaag
     3061 tagaacatcc tgagcaagat cttgagccta aatccatgcg caattactcg cgcatgaaaa
     3121 atcaggtcac ggtcagtatc aaccaaccgc tgaaatttga gaaaaaggat tacggttcat
     3181 tttatctctc gggaagctgg tcagattact gggcctccgg acaaaacagt actaattact
     3241 ctattggtta tagcaatagt gcgtcctggg gcagttacag tatcagtgct caacgttcgt
     3301 tgaatgaaga tggtcaaacc gacgatagca tttatctcag cttcacaatt ccaatagaaa
     3361 atttactggg tacggaacac cgcagctccg gcttccagag tattgataca caattaaaca
     3421 gcgactttaa gggtaataac caactcaata tcagcagtag tggttacagc gataccaatc
     3481 gcatcagcta cagcgtaaac acgggttata tgatgaataa atccagtgat gatttgagtt
     3541 atatcggcgg ttatgccagt tatgaatcgc cgtggggaac gctgtcaggt tcagcctcag
     3601 caagcagtga taatagtcgc caattctcac tcaatactga tggtggattt gtgttacata
     3661 gcggcgggct gacgttcagt aatgacagtt ttagtgattc cgacactctg gctgtcattc
     3721 aggcgccagg agcaaaaggc gctcgtatta actacggaaa cagcaccgta gaccgctggg
     3781 gttatggtgt gaccagcgcc ctttcgccat accatgaaaa tcgtatcgca ctggacatta
     3841 acgatctgga aaacgatgtt gaattgaaaa gcaccagtac tgtcgccgtc ccgcgccagg
     3901 gagccgtcgt ctttgctgat tttgaaacag ttcagggaca atcggcaatt atgaatatcg
     3961 tgcgaagcga tggtaaaaat attccatttg cagcggatat ttatgatgaa caaaacaata
     4021 tcatcggcaa cgtaggtcag ggtggtcagg cctttgttcg cggtatcggg caggaaggaa
     4081 atatacgaat tacctggatt gaagagggca aaccagtcag ttgttttgct cattaccaac
     4141 aaaacacgac atcagaaaaa atagcgcaat ctattattct gaacggactt cgttgtcaga
     4201 tacagtaacc acaagggact tccaatgata aaaaccacac cacataaaat agcaatactc
     4261 atggggctat tattatcgcc atctgtattt gcaacggatg ttaatgtcga ttttaccgct
     4321 accgtgaaag cgacaacatg taacatcacc ctgacatcgc tgaatggttc aaacgtcgtt
     4381 gataatggca atgatagtta taccctgata atccccaaga tgggactgga taagatagtc
     4441 aataaagcgt cacaatctga ggccaacttc aaattagtcg ccagtgggtg tagcagtgga
     4501 attagctgga ttgataccac aatgactgga aatcagtctg gtagtacatc gctgattatt
     4561 ccacaatctt ctgactcctc ctcaacaacc agtaatattg gtatgggatt taaaagatta
     4621 gagacttcgg gcgatacgtt tctgaaaccc aatagcgcgg aatacatccg ctggaaaacc
     4681 acagaaatta gcaccagtgg tctggaaatg actgtcgcac tgcgcgaaac aaccccagga
     4741 cagggcatac ccgggaaatt tcgcgcacag gcaacgttca atttcatcta tcaataacaa
     4801 ggatgacaat gatgatatta aaaaaattat gttatggatt atcgggtagt ctatttctgg
     4861 cttcatcact gttaaccatt tcctcacatg cggccactga tagtattggc ctgactgtta
     4921 ctacagacat agaaatgggt acctgtacat caacgttgac tgatgacggt acagctaaac
     4981 tgtccaccat caatttcggt gatgtatata tttctgaaat taatgccgaa actaaaatta
     5041 aaaccttcaa attgcagttc aaagactgtg cggggattcc cgggaaaaag gccaaaataa
     5101 agttgacccc acgagcctta tgcgaaggta attctaataa cggccctgga tttgctaacg
     5161 catcaacggc aacagcaaaa gcagcggcag tcgccgttga ggtctggagt acgtccacac
     5221 caggtaaaaa cggcgcaaaa caattcagtt gcgtcacccc cgcaacagaa gaagtcagta
     5281 ttgctggtgc gacgggaggt gatgttgtcg attacccgat gagtgctgtt ttggtggtcg
     5341 cgaaagataa aaccgttaca gatgtcaccg cgggtgattt caccgctccg gcaacattta
     5401 ctgtgactta taactaacag gcagaccgat tcaggaacta aatatgcgcc ccactcaacg
     5461 taatctgatg aaaaaatcaa ttctgcttct gtctttgcta ttcagcacca ctttccctgt
     5521 ggttgcagga caggatgttg acattgttgc aaatataaaa aataacacct gccaaagtgg
     5581 aatcagcaac aacggaaata ttgatttagg tgtcgttggc gtgggcgatt ttaccggaaa
     5641 catttcagcc gaaaattatc accccggtgg aaaagagttc actattacag tacaggactg
     5701 cacacttcag ggcacaggga atgtgttaaa tcagttacat attgattttc gcgcactcag
     5761 tggcgtaatg gcgacaggtt ccagtcaaat attcgccaat gaagacaaca ccggggctaa
     5821 aaatgttggg gtagttatat tctctattca ggacccggca aataccttca acgttcttgg
     5881 cgtaactggt agctcacgtt ccgtttatcc ggtcatgtca tcagcactaa acaattcatc
     5941 ctggaaattt agcactcgaa tgcaaaaaat cgatccagcg ctgaatgtca cctctggcca
     6001 acttataagc catgtgttag tggatattta ctacgaataa cagacaaagt gctgcaagca
     6061 ttatcttgca gcatgacaat ggagtaaaaa tgaagatatt cttcagatac tttctttttt
     6121 tagcactgtg ctcttgttgt tatacagcaa gtgttggtgc agctaataac gtcagctatg
     6181 ttgttgggaa taatgccggt gttggtccat cagaccaaaa atggaaagaa acaggcccca
     6241 atggagatgt caccgtcaaa ttccggtatg gttcaggcac caataattta gttttttata
     6301 aaccaaccca acttggccca accggggtgt cattaaaatg ggcgcagtta gattccgctt
     6361 caggtggagg tttcctttac tgtaacagga gtggtagcac aagcggcggg ccaatgcata
     6421 ttgaaaataa aatggttgat tccggtaaaa gttatggcgg acataaactc tttaagacat
     6481 ctgttccagg cctgtattac acgttagcaa tatcaaacat ttggtcaaca tatacatata
     6541 ctgatatcaa cccatcaggc atgtatattg gcgattctac gagccaatct tttaactggc
     6601 gtggcgaaag cgagcaaaca ctatattggt cttgcaacaa tgcgaataca aaaaataaat
     6661 attgggccgt tggcggcgtt atgcaaacgc tcacgattga attttatacc gataccgact
     6721 ttaacccgac aacgaatcaa cgggtaacat tatccagaac agataattac ctctactcat
     6781 tcaaagcata tggtgctggc ggtggtatca atgaacacag ctattttctt aaaatagact
     6841 ttgacctgac agatatcgta ttaacaaacc ctacctgttt tactgcggcg cttagtggct
     6901 catcggtaag tggttcaacg gttaaaatgg gtgattatac cccagcgcaa ataaaaaatg
     6961 gcgcaacagc ggttcctttt gatatcacgc tacagaactg tattcgtgtt cgcaatatag
     7021 aaacaaaact caagtcaaac aaggtgggta gtgtaagcaa agaacttctg gctaacacac
     7081 tcacaggaaa tgacgcagca aaaggcgttg gtgtactgat tgaaggttta aaaaacacta
     7141 aaagtgcaca aatggtactg aaaccaaatg atgcaacgtc tatttataaa gattatgaaa
     7201 cagaagatga cactactgga gggattttcc cggatcaagg caacgggacc tcacaacctc
     7261 ttcatttcca ggctacatta aaacaagacg gtaatattgc tatagaaccg ggggatttta
     7321 aagccaccag cacttttcag gttacctacc cctgataaca ctatcgcac
//
LOCUS       DAZFJL010000180         7290 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFJL010000180 DAZFJL010000000
VERSION     DAZFJL010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.182"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            227..1561
                     /gene="cadB"
                     /locus_tag="ACHJ8C_004253"
     CDS             227..1561
                     /gene="cadB"
                     /locus_tag="ACHJ8C_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO5554014.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            1641..3788
                     /gene="cadA"
                     /locus_tag="ACHJ8C_004254"
     CDS             1641..3788
                     /gene="cadA"
                     /locus_tag="ACHJ8C_004254"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO5554015.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAADDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            3847..5304
                     /gene="dtpC"
                     /locus_tag="ACHJ8C_004255"
     CDS             3847..5304
                     /gene="dtpC"
                     /locus_tag="ACHJ8C_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO5554016.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQSGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            5541..7058
                     /gene="lysS"
                     /locus_tag="ACHJ8C_004256"
     CDS             5541..7058
                     /gene="lysS"
                     /locus_tag="ACHJ8C_004256"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO5554017.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPLK"
     gene            complement(7177..>7290)
                     /gene="ghoT"
                     /locus_tag="ACHJ8C_004257"
     CDS             complement(7177..>7290)
                     /gene="ghoT"
                     /locus_tag="ACHJ8C_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO5554018.1"
                     /translation="IIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF"
BASE COUNT         1801 a         1690 c         1769 g         2030 t
ORIGIN      
        1 attgtaaaca ttgaatgttt atcttttcat gatatcaact tgcgatcctg atgtgttaat
       61 aaaaaacctc aagttctcac ttacagaaac ttttgtgtta tttcacctaa tctttaggat
      121 taatcctttt ttcgtgagta atcttagcgc cagtttggtc tggtcaggaa atagttatac
      181 atcatgaccc ggactccaaa ttcaaaaatg aaattaggag aagagcatga gttctgccaa
      241 gaagatcggg ctatttgcct gtaccggtgt tgttgccggt aatatgatgg ggagcggtat
      301 tgcattatta cctgcgaacc tagcaagtat cggtggtatt gctatctggg gttggattat
      361 ctctattatt ggtgcaatgt cgctggcata tgtatatgcc cgactggcaa caaaaaaccc
      421 gcaacaaggt ggcccaattg cgtatgccgg agaaatttcc cctgcatttg gttttcagac
      481 aggtgttctt tattaccatg ctaactggat tggtaacctg gcaattggta ttaccgctgt
      541 atcttatctt tccaccttct tcccagtatt aaatgatcct gttccggcgg gtatcgcctg
      601 tattgctatc gtctgggtat ttacctttgt aaatatgctc ggcggtacct gggtaagccg
      661 tttaaccact attggtctgg tgctggttct tattcctgtg gtgatgactg ctattgttgg
      721 ctggcattgg tttgatgcag caacttatgc agctaactgg aatactgcgg ataccactga
      781 tggtcatgcg atcattaaaa gtattctgct ctgcctgtgg gccttcgtgg gtgttgaatc
      841 cgcagcagta agtactggta tggttaaaaa cccgaaacgt accgttccgc tggcaaccat
      901 gctgggtact ggtttagcag gtattgttta catcgctgcg actcaggtgc tttccggtat
      961 gtatccgtct tctgtaatgg cggcttccgg tgctccgttt gcaatcagtg cttcaactat
     1021 cctcggtaac tgggctgcac cactggtttc tgcattcacc gcctttgcgt gcctgacttc
     1081 tctgggctcc tggatgatgt tggtaggcca ggcaggtgta cgtgccgcta acgacggtaa
     1141 cttcccgaaa gtttatggtg aagtcgacag caacggtatt ccgaaaaaag gtctgctgct
     1201 ggctgcagtg aaaatgactg ccctgatgat cctcatcact ctgatgaact ctgccggtgg
     1261 taaagcctct gacctgttcg gtgaactgac cggtatcgca gtactgctga ctatgctgcc
     1321 gtacttctac tcttgcgttg acctgattcg ttttgaaggc gttaacatcc gcaactttgt
     1381 cagcctgatc tgctctgtac tgggttgcgt gttctgcttc atcgcgctaa tgggcgcaag
     1441 ctccttcgag ctggcaggta ccttcatcgt cagcctgatt atcctgatgt tctacgctcg
     1501 caaaatgcac gagcgccaga gccactcaat ggataaccac acagcgtcta acgcacatta
     1561 attaaaagta ttttccgagg ctcctccttt cattttgtcc catgtgttgg gaggggcctt
     1621 ttttacctgg agatatgact atgaacgtta ttgcaatatt gaatcacatg ggggtttatt
     1681 ttaaagaaga acccatccgt gaacttcatc gcgcgcttga acgtctgaac ttccagattg
     1741 tttacccgaa cgaccgtgac gacttattaa aactgatcga aaacaatgcg cgtctgtgcg
     1801 gcgttatttt tgactgggat aaatataatc tcgagctgtg cgaagaaatt agcaaaatga
     1861 acgagaacct gccgttgtac gcgttcgcta atacgtattc cactctcgat gtaagcctga
     1921 atgacctgcg tttacagatt agcttctttg aatatgcgct gggtgctgct gatgatattg
     1981 ctaacaagat caagcagacc actgacgaat atatcaacac tattctgcct ccgctgacca
     2041 aagcactgtt taaatatgtt cgtgaaggta aatatacttt ctgtactcct ggtcacatgg
     2101 gtggtactgc attccagaaa agcccggtag gtagcctgtt ctatgatttc tttggtccga
     2161 acaccatgaa atctgatatt tccatttcag tatctgaact gggttctctg ctggatcaca
     2221 gtggtccaca caaagaagca gaacagtata tcgctcgcgt ctttaacgca gaccgcagct
     2281 acatggtgac caacggtact tccactgcga acaaaattgt tggtatgtac tctgctccgg
     2341 caggcagcac cattctgatt gaccgtaact gccacaaatc gctgacccac ctgatgatga
     2401 tgagcgatgt tacgccaatc tatttccgcc cgacccgtaa cgcttacggt attcttggtg
     2461 gtatcccaca gagtgaattc cagcacgcta ccattgctaa gcgcgtgaaa gaaacaccaa
     2521 acgcaacctg gccggtacat gctgtaatta ccaactctac ctatgatggt ctgctgtaca
     2581 acaccgactt catcaagaaa acactggatg tgaaatccat ccactttgac tccgcgtggg
     2641 tgccttacac caacttctca ccgatttacg aaggtaaatg cggtatgagc ggtggccgtg
     2701 tagaagggaa agtgatttac gaaacccagt ctactcacaa actgctggcg gcgttctctc
     2761 aggcttccat gatccacgtt aaaggtgacg taaacgaaga aacctttaac gaagcctaca
     2821 tgatgcacac caccacctct ccgcactacg gtatcgtggc gtccactgaa accgctgcgg
     2881 cgatgatgaa aggcaatgca ggtaaacgtc tgatcaatgg ttccattgaa cgtgcgatca
     2941 aattccgtaa agagatcaaa cgtctgagaa cggaatctga tggctggttc tttgatgtat
     3001 ggcagccgga tcatatcgat acgactgaat gctggccgct gcgttctgac agcacctggc
     3061 acggcttcaa aaacatcgat aacgagcaca tgtatcttga cccgatcaaa gtcaccctgc
     3121 tgactccggg gatggaaaaa gacggcacca tgagcgactt tggtattccg gccagcatcg
     3181 tggcgaaata cctcgacgaa catggcatcg ttgttgagaa aaccggtccg tataacctgc
     3241 tgttcctgtt cagcatcggt atcgataaga ccaaagcact gagcctgctg cgtgctctga
     3301 ctgacttcaa acgtgcgttc gatctgaacc tgcgtgtgaa aaacatgctg ccgtctctgt
     3361 atcgtgaaga tcctgaattc tatgaaaaca tgcgtattca ggaactggct cagaatatcc
     3421 acaaactgat tgttcaccac aatctgccgg atctgatgta tcgcgcattt gaagttctgc
     3481 cgactatggt aatgactccg tatgctgcgt tccagaaaga gctgcacggt atgaccgaag
     3541 aagtttacct cgacgaaatg gtaggtcgta ttaacgccaa tatgatcctt ccgtatccgc
     3601 cgggagttcc tctggtaatg ccgggtgaaa tgatcaccga agaaagccgt ccggttctgg
     3661 agttcctgca gatgctgtgt gaaatcggcg ctcactatcc gggctttgaa accgatattc
     3721 acggtgcata ccgtcaggct gatggccgct ataccgttaa agtattgaaa gaagaaagca
     3781 aaaaataatt agctcgtaca agggaagtgg cttgccactt cccttttttg ctcataagga
     3841 gaacacatga aaacaccctc acagccgcgc gcgatatact atatcgtggc gatccaaatc
     3901 tgggagtact tcagttttta cggcatgcgt gccttactca ttctctatct cacccatcag
     3961 cttggttttg atgataacca tgccatcagc ctgttcagcg catatgcttc tctggtttac
     4021 gttaccccta ttctcggcgg ctggcttgcc gaccgcctgc tcggcaaccg cactgcagtg
     4081 attgccggcg cgctgttaat gacccttggc catgtggtgc tgggcattga tacaaattca
     4141 acctttagcc tgtatctggc gctggcaatc attatttgtg gctacggttt attcaaatca
     4201 aacatcagct gtttgcttgg cgagctctac gacgagaacg atcatcgacg tgatggcggt
     4261 ttttcgctgc tgtatgctgc gggcaatatc ggttctatcg ccgcgcccat cgcctgcggc
     4321 ctggctgctc agtggtatgg atggcatgtt ggctttgccc ttgcgggagg cggcatgttt
     4381 atcggtttgt tgattttctt aagtggtcat cgtcatttcc agtccacgcg tagtatggat
     4441 aaaaaagcgc tcacaagcgt caaatttgcc ttaccagtat ggagctggtt agtggtgatg
     4501 ctctgtttag cccctgtatt ttttaccctg ctgctggaga acgactggtc cggatatttg
     4561 ctggcgatcg tttgcctcat tgcagcacaa atcattgccc gcatgatgat caaattcccc
     4621 gaacaccgcc gtgctctttg gcaaattgta ttgttgatgt ttgtcgggac gttgttctgg
     4681 gtactggcac aacagagcgg cagtaccatc agcttgttta tcgatcgctt tgtgaatcgt
     4741 caggcattca atattgaagt acctacagca ctattccagt cggtgaatgc tattgcggtg
     4801 atgctcgctg gggttgttct ggcctggctg gcgtcgccag aaagccgcgg caattcagca
     4861 ttgcgcgtct ggctgaagtt tgcctttggc ttactgctga tggcttgtgg ctttatgttg
     4921 ctggcatttg atgcccgaca tgcagcggct ggcggtcaag cgtcaatggg cgtgatgata
     4981 tccgggctgg cgctaatggg ctttgctgaa ctctttattg acccggtggc gattgcgcaa
     5041 atcacgcgtc tgaaaatgtc tggcgtatta actggtattt atatgctggc aacaggcgca
     5101 gtcgccaact ggctggcagg cgtcgtggca cagcagacga cagagtcgca aattagcggt
     5161 atggctattg ccgcttacca gcgattcttt tctcagatgg gagagtggac gttggcttgt
     5221 gtcgcgatca tcgtggtatt ggcctttgct acccgttttc tgtttagcac gccgacgaat
     5281 atgatacagg agagcaacga ttaactgtcg taaaaacaat tggttatgaa taaattaact
     5341 ttcagtttat cacactagta aatcgagatt aataagtcat ttaaaccatt ttgatggtta
     5401 tttattagtg atatcaactt gaggtaagcg ttagtttcga taagataaac tgagttatta
     5461 atagtcgagg cagataatac agtgtaccga tctgtctctt tatctacgct aaattgaaag
     5521 ctggatttag aggaaccaaa atgtctgaac gagaaacacg gggagccaat gaggctattg
     5581 attttaacga tgaactgaga aatcgccgcg aaaaactggc ggcactacgt cagcaaggtg
     5641 tggcgtttcc caatgatttt cgccgcgacc atacctctga ccagttgcac gaagagtttg
     5701 atgcgaagga taaccaggaa ctggaatcct taaacattga agtctcggtt gctggccgaa
     5761 tgatgacccg tcgtattatg gggaaagcct cctttgtaac gttgcaggat gtcggtggcc
     5821 gtattcaact gtacgttgca agagatagcc tgccagaagg tgtttataac gatcagttta
     5881 aaaaatggga tctgggtgac attatcggtg cccgcggtac gctgtttaag acgcaaacgg
     5941 gtgagctttc cattcactgt actgagctgc gcctgctgac taaagcacta cgtcctttac
     6001 cagataaatt ccatggtctg caggatcagg aagtccgtta tcgtcaacgt tatctggacc
     6061 tcatcgctaa cgataaatcc cgtcaaacgt ttgttgtccg ttcaaaaatt ctggccgcta
     6121 tccgtcagtt catggtcgcg cgcggcttta tggaagtaga aaccccgatg atgcaggtaa
     6181 ttccaggtgg ggcatctgct cgcccgttta ttacccatca taacgctctg gatttagata
     6241 tgtacctgcg tatcgcgccg gagctgtatc tgaaacgtct ggttgtaggc ggttttgaac
     6301 gggtattcga aatcaaccgt aacttccgta atgaaggtat ttctgttcgc cataatcctg
     6361 agttcacaat gatggaactc tacatggcat atgcggatta ccacgatttg attgaactga
     6421 cggagtcact gttccgcacc ctggcacaag aggtactggg taccactaaa gtcacttatg
     6481 gcgagcatgt gtttgatttc ggcaaaccgt ttgaaaaact caccatgcgc gaagcaatca
     6541 aaaaatatcg tccagaaacc gatatggcgg atctggataa ttttgatgct gctaaagcat
     6601 tagctgaatc tatcggtatt acggtagaga aaagctgggg gctgggacgt attgttacgg
     6661 agatctttga tgaagtggca gaagcacatc tgattcagcc aacctttatt acggaatatc
     6721 cggcagaagt gtccccgctg gcacgccgta atgatgttaa cccggaaatc accgaccgtt
     6781 ttgaattctt catcggtggt cgtgaaattg gtaatggttt tagcgaatta aacgacgcag
     6841 aagatcaggc tgaacgtttc caggaacagg ttaacgctaa agctgcaggt gacgacgaag
     6901 ccatgttcta tgacgaagat tacgtgactg cgctggaata tggtctgccg ccaaccgctg
     6961 gtctgggtat tggtatcgac cgaatgatta tgctgtttac aaacagtcat actattcgcg
     7021 acgttattct cttcccggcg atgcgcccac tgaaataaat ttcactttaa tgaacgaagc
     7081 agtcaggcga ctgcttcgtt cgtcattccg caaatccagt tttgaggaga gacgcttcgt
     7141 ttcaagataa ccagcgggtc cactcaacta cagctcctaa aagagagaaa aaagtaatgc
     7201 cacaggcaga ctcattggcc aggttattcc gaccagaaat gcactaagta aacgaatatg
     7261 tgttttctca tgtgagataa accagataat
//
LOCUS       DAZFJL010000181         7275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZFJL010000181 DAZFJL010000000
VERSION     DAZFJL010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.183"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..119)
                     /locus_tag="ACHJ8C_004258"
     CDS             complement(<1..119)
                     /locus_tag="ACHJ8C_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO5554019.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDA"
     gene            complement(125..421)
                     /locus_tag="ACHJ8C_004259"
     CDS             complement(125..421)
                     /locus_tag="ACHJ8C_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO5554020.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRCGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQFDIDSEV"
     gene            complement(497..640)
                     /gene="kil"
                     /locus_tag="ACHJ8C_004260"
     CDS             complement(497..640)
                     /gene="kil"
                     /locus_tag="ACHJ8C_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO5554021.1"
                     /translation="MDQTIMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIH"
     gene            complement(609..773)
                     /locus_tag="ACHJ8C_004261"
     CDS             complement(609..773)
                     /locus_tag="ACHJ8C_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO5554022.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNNYGYPD"
     gene            complement(846..1214)
                     /locus_tag="ACHJ8C_004262"
     CDS             complement(846..1214)
                     /locus_tag="ACHJ8C_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HFO5554023.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDSDDMTIKAA"
     gene            complement(1397..1597)
                     /locus_tag="ACHJ8C_004263"
     CDS             complement(1397..1597)
                     /locus_tag="ACHJ8C_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction Ral family protein"
                     /protein_id="HFO5554024.1"
                     /translation="MTTTIDKNQWCGQFKRCNGCKLQSECMVKPEEMFPVMEDGKYVD
                     KWAIRTTAMIARELGKQNNKAA"
     gene            complement(1726..1998)
                     /locus_tag="ACHJ8C_004264"
     CDS             complement(1726..1998)
                     /locus_tag="ACHJ8C_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFO5554025.1"
                     /translation="MSRKTEFKGTAASRRRARRANLQSQKAISSDKLHRPTPSRVVLQ
                     CKRKPAMRAEVITLTTLTRKYEGSTCLPNVALYAAGYRKSKQLTAR"
     gene            complement(1976..2156)
                     /locus_tag="ACHJ8C_004265"
                     /pseudo
     CDS             complement(1976..2156)
                     /locus_tag="ACHJ8C_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502599.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2441..2881)
                     /locus_tag="ACHJ8C_004266"
     CDS             complement(2441..2881)
                     /locus_tag="ACHJ8C_004266"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFO5554026.1"
                     /translation="MKGTKKWDELTNDDFYYDIKQKQVDIKLGMDITTLAYEKLVDVI
                     VLVAGDSDFVPAAKHARIKGIDFILDPLRQNVTPSLSEHIDGVQSYSLISGLADALHV
                     EPDPAPDWWEDRKKGKPRGKNNSGKRRYGNTQAESAKKHQRNKR"
     gene            complement(2951..3298)
                     /locus_tag="ACHJ8C_004267"
                     /pseudo
     CDS             complement(2951..3298)
                     /locus_tag="ACHJ8C_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308300.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3426..4121)
                     /locus_tag="ACHJ8C_004268"
     CDS             complement(3426..4121)
                     /locus_tag="ACHJ8C_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO5554027.1"
                     /translation="MDGCNTERNNKDMKMKWYELARSRMKELGITQEKLAEELGMTQG
                     GIGHWLRGSRHPSLSDIGVVFKYLGIDNISFNHDGTFSPVGEYSSAPVKKQYEYPVFS
                     HVQAGMFSPELRTFTKGDAERLVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPDGML
                     ILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSVVG
                     KVIASQWPEETFG"
     gene            4196..4411
                     /locus_tag="ACHJ8C_004269"
     CDS             4196..4411
                     /locus_tag="ACHJ8C_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5554028.1"
                     /translation="MSNLRKYRESLNISQTTLAKAVGCTQGAIGHWESGRRFPDLKTC
                     RALVACLNKLGAKVSLDDVFPPEHKAA"
     gene            4553..4849
                     /locus_tag="ACHJ8C_004270"
     CDS             4553..4849
                     /locus_tag="ACHJ8C_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO5554029.1"
                     /translation="MEQTNYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITNKKRPAATERSEQIQMEF"
     gene            4882..5781
                     /locus_tag="ACHJ8C_004271"
     CDS             4882..5781
                     /locus_tag="ACHJ8C_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO5554030.1"
                     /translation="MTNTAKILNFGRGNFAGQERNVADLDDGYARLSNMLLEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAKLELVRMNIIKQQ
                     GGMFGPNKNISEWCIPQNEGKSPKTRDKTSLKLGDCYPSKQGDTKDTITKEKRKDYSS
                     ENSGESSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFA
                     GWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQ
                     AVVTASKPKLDLTNTDWIYGVDL"
     gene            5778..6479
                     /locus_tag="ACHJ8C_004272"
     CDS             5778..6479
                     /locus_tag="ACHJ8C_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO5554031.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELVHMTARINRGEAIPEPVKQLPVMGGRPLNRVQALAK
                     IAEIKAKFGLKGASV"
     gene            6476..6766
                     /locus_tag="ACHJ8C_004273"
     CDS             6476..6766
                     /locus_tag="ACHJ8C_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO5554032.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATKEEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            6840..>7275
                     /locus_tag="ACHJ8C_004274"
     CDS             6840..>7275
                     /locus_tag="ACHJ8C_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO5554033.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVSEFAELLELIQAFGTERGVKWSDEARPALEWKARWGDRA"
BASE COUNT         1946 a         1651 c         1699 g         1979 t
ORIGIN      
        1 ctggcatcac ctttaaatgc cgtctggcga agagtggtga tcagttcctg tgggtcgaca
       61 gaatccatgc cgacacgttc agccagcttc cctgccagcg ttgcgagtgc tgtactcatc
      121 cgttttatac ctctgaatca atatcaaact ggtggtgagc aatggtttca accatgtacc
      181 ggatgtgttc tgccatgcgc tcctgaaact caacatcgtc atcaaacgca cgggtaatgg
      241 cttttttgct ggcaccgcag cgttgcaaat gatcgataca gagtgattca aacaggtgct
      301 gtggaagacc tttttccatg tcgtctgcca gttctgcctc tttctcttca cgggcgatct
      361 gctggtagtg acgcgcccag ctctgagcct caagacgatc ctgaatgtaa taagcgttca
      421 tggctgaact cctgaaaatg gctgtgaaaa tatcgcccgc gaaatgccag gctgattagg
      481 aaaacaggaa agggggttag tgaatgcttt tgcttgatct cagtttcagt attaatatcc
      541 attttttata agcgtcgacg gcctcacgaa acatcttttc atcgccaata aaagtggcga
      601 tagtgaattt agtctggata gccataattg tttgatccat tttttgggac tcctggctga
      661 ttaagtatgt cgataaggcg tttccatccg tcacgtaatt tacgggtgat tcgttcaagt
      721 aaagattcgg aagggcagcc agcaacaggc caccctgcaa tggcatattg catggtgtgc
      781 tccttattta tacataacga aaaacgcctc gagtgaagcg taattggtat gcggtaacgc
      841 cgcgctcagg cggccttgat agtcatatca tctgaatcaa atattcctga tgtatcgata
      901 tcggtaattc ttattccttc actaccatcc attggaggcc atccttcctg accatttcca
      961 tcattccagt cgaactcaca cacaacacca tatgcattta agtcgcttga aattgctata
     1021 agcagagcat gttgcgccag catgattaat acagcattta atacagagcc gtgtttattg
     1081 agtcggtatt cagagtctga ccagaaatta ttaatctggt gaagtttttc ctctgtcatt
     1141 acgtcatggt caatttcaat ttctattgat gctttccagt cgtaatcaat gatgtatttt
     1201 ttgatgtttg acatctgttc atatcctcat agataaaaaa tcgccctcac actggagggc
     1261 aaagaagatt tccaataatc agaacaagtc ggctcctgtt tagttacgag cgacattgct
     1321 ccgtgtattc actcgttgga atgaatacac agtgcagtgt ttattctgtt atttatacca
     1381 aaaataaagg ccactatcag gcagctttgt tgttctgttt accaagttct ctggcaatca
     1441 ttgccgtcgt tcgtattgcc catttatcga catatttccc atcctccatt acagggaaca
     1501 tttcttcagg cttaaccatg cattccgatt gcagcttgca tccattgcat cgtttgaatt
     1561 gtccacacca ttgattctta tcaatagtcg tagtcatacg gatagtcctg gtattgttcc
     1621 atcacatcct gaggatgatc ttcgaactct tcaaattctt cttccatatc tcacctcaaa
     1681 taagtggttt gctgcctaat ttcattttct ggcgaccaac acaagtcatc ttgctgtcag
     1741 ttgtttggat ttccggtagc ctgccgcgta aagagctacg tttggaagac aagttgagcc
     1801 ttcatatttt ctggtcagcg ttgtcagtgt tattacttct gctctcattg ctggtttgcg
     1861 cttgcattgc aagaccactc gtgaaggggt tggcctgtgt agcttgtcgg agctgatcgc
     1921 cttctgactt tgcagatttg cgcgacgagc tctacggcga gaagctgcgg tgcctttaaa
     1981 ttctgttttt ctggacatag attcctcccg aataaacttt ggcgatgcaa tctcgaagct
     2041 cctcctgaga cggttgcttc ggcattgcat cccacagctc atgtggttgg gtgatctggc
     2101 ttttcagcca cgtagtcgag agttcgacgt tgtttaaaga gcctgccagt ctgttccgtt
     2161 tggcttccag cgtcctactg acggttaaat agtacgatat gtactttatt tggtcaatac
     2221 gatttgttct aaaatgaggt gattttttat aacactttgt atttaatagt gttgtttttt
     2281 agcgtgggtg tatcgcctcg gcgatgtaag gagagatcag aattgcgttg tttagtgagt
     2341 tgcacctatt aattttaaaa taaatacaat tggttatgtg tttttaggtg gggcgatcgt
     2401 gaggcaaaga aaacccggct cggtggccgg gttgatggga ttatcgttta tttctttgat
     2461 gtttctttgc agactcagct tgagtgtttc catacctgcg tttaccgcta ttgttttttc
     2521 ctctaggctt gccttttttt cgatcttccc accagtcagg tgccgggtct ggctcaacat
     2581 gtaaagcatc ggcaagtcct gatatcaagc tgtatgactg aactccatca atgtgctctg
     2641 acagtgatgg ggtaacattc tgtcttagtg gatcaagaat aaaatcaata cctttaattc
     2701 tggcgtgttt ggcggcaggc acaaagtctg agtccccagc aacaaggaca attacatcaa
     2761 caagtttttc ataagctaaa gtcgtgatat ccatccctag cttgatatca acttgttttt
     2821 gtttgatgtc atagtaaaaa tcatcatttg tcagttcatc ccattttttc gttcctttca
     2881 tcagagcatc aagggagaat gtagttagtt gccatcgctt attatcaaca agattcccta
     2941 atcttaaggc cgtttttcga gtttttctta actcttcatg cagctccgtt ctcagaatat
     3001 atgatttttc gagtttgaaa ttcttgcgac caggcgtctt attgctaggc tcaggaagtg
     3061 gaaggcgtgt ttgaatgtcg agtggtggac agtcatagaa gtaaattcta taaagctcaa
     3121 gtggttccct acgttcttgt gattgacgtt ttccattaag atgggaaaga accattgacc
     3181 atattacttt cattatgcat tgcgcagtca gttcatgctc ggcgaagtgt ttacgatgcg
     3241 tagcatgaac acgctgcatg aaaaaacccg catcaattaa aattgctgct ttcttcataa
     3301 gagtcccaaa aaaatagccc agagccgtta tgcagatatt aacaattgtc tgcgaacggg
     3361 gctgggcttg gtttaattaa tctatgccaa gttgactgtg ccgtcaacat aaaacttact
     3421 tcctatcacc caaacgtctc ttctggccac tggctggcga taactttccc cacaacggaa
     3481 caactctcat tgcatgggat cattggatat tgcgggttta gtggctgtag aaacacctga
     3541 ccgctatccc tgatcagttt cttgaaggta aactcatcac caccaagtct ggctatgcag
     3601 aaatcacctg gctcaacagc ctgctcaggg tcaaccagaa ttaacatccc gtcaggaaaa
     3661 ctaggtttgg aacctgttgg ggcggtcatt gagttacctt caacctcaag ccagaatgca
     3721 gagtcactgg cttttttggt tgtgcttacc aatctctccg catcgccttt ggtaaaggtt
     3781 ctgagttctg gagagaacat cccagcctga acatgagaaa aaacagggta ctcatattgt
     3841 tttttaactg gggccgatga gtattcgcca acaggtgaaa atgtcccgtc gtggttgaat
     3901 gatatgttat caataccaag gtatttaaac accacaccaa tatcactaag agatggatga
     3961 cgagatccgc gcaaccagtg tccaattcca ccctgcgtca tacctagctc ttcggctaac
     4021 ttctcttgag ttatgccgag ctctttcatt ctggatctag ccagttcata ccatttcatt
     4081 ttcatgtcct tattattacg ctctgtattg caaccatcca tgcacaatgt gtatttttgc
     4141 ttgcattcaa aaagtacata ttgtattttt tcttcatggt tactatggag ggcatatgag
     4201 caacctacga aaatatcgag agtcactgaa tatctctcaa acaacacttg ctaaggcagt
     4261 tggatgcaca cagggagcta tcggacattg ggaatctggt cgtcgcttcc cagaccttaa
     4321 aacatgccgt gctcttgttg cgtgtctaaa caagttaggc gcaaaagtaa gtcttgatga
     4381 cgtgttcccg ccggagcaca aagccgctta ataagcggag ccgctctttg taacaacgga
     4441 cattcgtcct acgtcgctga aaagcgagcc ccaagatatc tgaccaacta aggccatatg
     4501 cgtttccacg catacctttc aactaactat tcactattgg aaatcttaag aaatggaaca
     4561 aacaaattac agcaaactat cacagcgcga cgttgatcgc gcagaaacag atttacttat
     4621 caacctgtca acgcttactc agcgcggtct ggcaaagatg attggctgtc atgaatcgaa
     4681 gataagcaga acagactgga ggtttatagc ttcggtcttg tgtgcttttg gcatggcatc
     4741 agacatcagt ccgattagca gagcttttaa gtatgcgctt gatgaaatca ccaataaaaa
     4801 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gaattctgag gtcattactg
     4861 gatctatcaa caggagtcat tatgacaaat acagcaaaaa tactcaactt cggcagaggt
     4921 aactttgccg gacaggagcg taatgtggca gatctcgatg atggttacgc cagactatca
     4981 aatatgctgc ttgaggctta ttcaggcgca gatctgacca agcgacagtt taaagtgctg
     5041 cttgccattc tgcgtaaaac ctatgggtgg aataaaccaa tggacagaat caccgattct
     5101 caacttagcg agattacaaa gttacctgtc aaacggtgca atgaagccaa gttagaactc
     5161 gtcagaatga atattatcaa gcagcaaggc ggcatgtttg gaccaaacaa aaacatctca
     5221 gaatggtgca tccctcaaaa cgagggaaaa tcccctaaaa cgagggataa aacatccctc
     5281 aaattggggg attgctatcc ctcaaaacag ggggacacaa aagacactat tacaaaagaa
     5341 aaaagaaaag attattcgtc agagaattct ggcgaatcct ctgaccagcc agaaaacgac
     5401 ctttctgtgg tgaaaccgga tgctgcaatt cagagcggca gcaagtgggg gacagcagaa
     5461 gacctgaccg ccgcagagtg gatgtttgac atggtgaaga ccatcgcgcc atcagccaga
     5521 aaaccgaatt ttgctgggtg ggctaacgat atccgcctga tgcgtgaacg tgacggacgt
     5581 aaccatcgcg acatgtgtgt acttttccgc tgggcctgcc aggacaactt ctggtccggt
     5641 aacgtgctga gtccggccaa actccgcgac aagtggaccc agctcgaaat caaccgtaac
     5701 aagcaacagg cagtcgtgac agccagcaaa ccaaaactcg acctgacaaa cacagactgg
     5761 atttacgggg tggatctatg aaaaacatcg ccgcacagat ggttaacttt gaccgtgagc
     5821 agatgcgtcg gatcgccaac aacatgccgg aacagtacga cgaaaagccg caggtacagc
     5881 aggtagcgca gatcatcaac ggtgtgttca gccagttact ggcaactttc ccggcgagcc
     5941 tggctaaccg tgaccagaac gaactgaacg aaatccgccg ccagtgggtg ctggctttcc
     6001 gggaaaacgg gatcaccaca atggaacagg ttaacgcagg aatgcgcgta gcccgtcggc
     6061 agaatcgacc atttctgcca tcacccgggc agtttgttgc atggtgccgg gaagaagcat
     6121 ccgttatcgc cggactgcca aacgtcagcg agctggttga tatggtttac gagtattgcc
     6181 ggaagcgagg cctgtatccg gatgcggagt cttatccgtg gaaatcaaac gcgcactact
     6241 ggctggttac caacctgtat cagaacatgc gggccaatgc gcttactgat gcggaattac
     6301 gccgtaaggc cgcagatgag cttgtccata tgactgcgag aattaaccgt ggtgaggcga
     6361 tccctgaacc agtaaaacaa cttcctgtca tgggcggtag acctctaaat cgtgttcagg
     6421 cgctggcgaa gatcgcagaa attaaagcta agttcggact gaaaggagca agtgtatgac
     6481 gggcaaagag gcaattattc attacctggg gacgcataat agcttctgtg cgccggacgt
     6541 tgccgcgtta acaggcgcaa cagtaaccag cataaatcag gccgcggcta aaatggcacg
     6601 ggcaggtctt ctggttatcg aaggtaaggt ctggcgaacg gtgtattacc ggtttgctac
     6661 caaggaagaa cgggaaggaa agatgagcac gaacctgatt tttaaggagt gtcgccagag
     6721 tgccgcgatg aaacgggtat tggcggtata tggagttaaa agatgaccat ctacatcact
     6781 gagctaatga caggcctgct ggtaatcgca ggccttttta tttgggggag agggaagtca
     6841 tgaaaaaact aacctttgaa attcgatctc cagcacatca gcaaaacgct attcacgcag
     6901 tacagcaaat ccttccagac ccaaccaaac caatcgtagt aaccattcag gaacgcaacc
     6961 gcagcttaga ccaaaacagg aagctatggg cctgcttagg tgacgtctct cgtcaggttg
     7021 aatggcatgg tcgctggctg gatgcagaaa gctggaagtg tgtgtttacc gcagcattaa
     7081 agcagcagga tgttgttcct aaccttgccg ggaatggctt tgtggtaata ggccagtcaa
     7141 ccagcaggat gcgtgtaagc gaatttgcgg agctattaga gcttatacag gcattcggta
     7201 cagagcgtgg cgttaagtgg tcagacgaag cgcgaccggc tctcgaatgg aaagcgcgat
     7261 ggggagacag ggcgg
//
LOCUS       DAZFJL010000182         7207 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZFJL010000182 DAZFJL010000000
VERSION     DAZFJL010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.184"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(129..296)
                     /locus_tag="ACHJ8C_004275"
     CDS             complement(129..296)
                     /locus_tag="ACHJ8C_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554034.1"
                     /translation="MHFRVTGEWNGEPFNRVIETENINDCYDHWIIWAQIAHADITNI
                     RIEELKEHQAA"
     gene            complement(439..897)
                     /locus_tag="ACHJ8C_004276"
     CDS             complement(439..897)
                     /locus_tag="ACHJ8C_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/biocide efflux PACE transporter"
                     /protein_id="HFO5554035.1"
                     /translation="MQHNAIQRRSLLERIFHAICFEGIATAILAPTTAWLMQRTILEM
                     GGMTILLASTAMIWNIIYNMLFDRYWPAHQVKRTAKVRAFHALGFESGFIAIGVIMVS
                     WILSVSLLQAFTLEIGFFLFFLPYTMFYNWAYDSLRLRVVKQHQQRMLAR"
     gene            1001..1885
                     /locus_tag="ACHJ8C_004277"
     CDS             1001..1885
                     /locus_tag="ACHJ8C_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5554036.1"
                     /translation="MRYSLEALSTFVEAVSCGSFSAAARRLRKSQSAVSTSIANLEVD
                     LGISLFDRSSREPVLTEEGKKVLSYVHAILSASERLEEVAVSLAGETEARLTFVLSDT
                     LHPDLLEDIMVQFDSQFPHTELEVLIGEDVDVIDLLQKERAQIGLIESREHYPTDIGS
                     TRLPMQAWMGIYVSASHPLSAEKTIQREQLHSWRELRLNTYIDNREMIARGPVWSAPN
                     YLLLLSMAVQGFGWCSLPCALVEEFATEKPLVQLDVPGWPKAISIDLLWNKKSPPGAA
                     GNWLKHHLQHINAPNSRT"
     gene            complement(2087..2770)
                     /locus_tag="ACHJ8C_004278"
     CDS             complement(2087..2770)
                     /locus_tag="ACHJ8C_004278"
                     /inference="COORDINATES: protein motif:HMM:NF016342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dATP/dGTP pyrophosphohydrolase domain-containing
                     protein"
                     /protein_id="HFO5554037.1"
                     /translation="MASLEREQIRHEHAKWSDSTFGCVGPIGPLKHLSKEALEAAAEP
                     DDLSEWADMQFLLWDAQRRAGISDAEINVAMEDKLKINMERQWPEPKDGEPRLHIKEP
                     GNSPVIPDGLSTVCAEAYQVVGVMADALGVFGDAAVQKVLDNLSQQKLVHRDVLPFSL
                     PVTPDSWISCSERMPNDAQWCVVNTEYGYYAQCWSEGQGWLGDDISIPECDVINWMPL
                     PEPPQEVNQ"
     gene            complement(2744..2869)
                     /locus_tag="ACHJ8C_004279"
                     /pseudo
     CDS             complement(2744..2869)
                     /locus_tag="ACHJ8C_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF550 domain-containing protein"
     gene            complement(2866..3075)
                     /locus_tag="ACHJ8C_004280"
     CDS             complement(2866..3075)
                     /locus_tag="ACHJ8C_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554038.1"
                     /translation="MAIAASYTMHLYCDCRQCTEGVYPVPDFGEYIGTSWSGCAKEAR
                     KDGWRISKDKTRTFAPGHKVLRINT"
     gene            complement(3077..3625)
                     /locus_tag="ACHJ8C_004281"
     CDS             complement(3077..3625)
                     /locus_tag="ACHJ8C_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5554039.1"
                     /translation="MSEINSQALREAAEQAMHDDWGFDADLFHELVTPSIVLALLDER
                     ESQNKRIAELEKSEEQLINERDHAESALDDMYFAATGNRPEWSNWFGFSDAVDAVVDR
                     IADLEAKQCIEEMSKQSCEARERDLFESWVMHSICISKSTLEGLRTETGYRNATLSGT
                     DFNRMWEQWKSIRAAGIRIKGD"
     gene            complement(3622..3843)
                     /locus_tag="ACHJ8C_004282"
     CDS             complement(3622..3843)
                     /locus_tag="ACHJ8C_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO5554040.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(3942..4157)
                     /locus_tag="ACHJ8C_004283"
     CDS             complement(3942..4157)
                     /locus_tag="ACHJ8C_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO5554041.1"
                     /translation="MTPQQENALRSIARQANYEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            complement(4234..4425)
                     /locus_tag="ACHJ8C_004284"
     CDS             complement(4234..4425)
                     /locus_tag="ACHJ8C_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO5554042.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLAASL
                     SQKLEMMVAKAEADERDQV"
     gene            complement(4398..4580)
                     /locus_tag="ACHJ8C_004285"
     CDS             complement(4398..4580)
                     /locus_tag="ACHJ8C_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO5554043.1"
                     /translation="MKHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLPLS"
     gene            complement(4577..5257)
                     /locus_tag="ACHJ8C_004286"
     CDS             complement(4577..5257)
                     /locus_tag="ACHJ8C_004286"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO5554044.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            complement(5254..6039)
                     /gene="bet"
                     /locus_tag="ACHJ8C_004287"
     CDS             complement(5254..6039)
                     /gene="bet"
                     /locus_tag="ACHJ8C_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO5554045.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            complement(6045..6341)
                     /locus_tag="ACHJ8C_004288"
     CDS             complement(6045..6341)
                     /locus_tag="ACHJ8C_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO5554046.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            6506..7069
                     /locus_tag="ACHJ8C_004289"
                     /pseudo
     CDS             6506..7069
                     /locus_tag="ACHJ8C_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
BASE COUNT         1771 a         1801 c         1677 g         1958 t
ORIGIN      
        1 ttctttttca gtccacatgc gattgaggca agagttgcgc tactgatgcc aacatcgcca
       61 gtcgattgcc atttcaaaac cttcatagcc aaatctgaca tttcttgtct ccaataaaaa
      121 aaccgccatc aggcggcttg gtgttctttc agttcttcaa ttcgaatatt ggttatgtct
      181 gcatgtgcta tctgcgccca tattatccag tggtcatagc agtcgttgat gttctctgtt
      241 tcgataaccc tgttgaatgg ctctccattc cattctcctg tgactcggaa gtgcatttat
      301 catccccata aaacaaaact cgccgtagcg agttcagata aaagaaatcc ccgcgagggc
      361 ggggatgtta agtcggtggg ggtacagtat ggatatcgaa aattattgcg cgattttcat
      421 tactcccctg ggaaaacatt atctggcaag catacgttgt tgatgctgtt tcacaacacg
      481 taaacgtaag ctgtcatagg cccaattgta gaacatggta tatggtagaa agaaaaggaa
      541 gaatcctatc tctagtgtaa aagcctgtaa cagactgacg ctcagaatcc aggacaccat
      601 aatgacgccg atcgcgataa atccactttc aaagcccaaa gcatggaatg cacgaacttt
      661 tgcagttcgt tttacctgat gagcaggcca gtagcgatca aatagcatgt tatagatgat
      721 attccagatc attgcagttg aagccaacaa gatagtcatc ccgcccattt cgagtatagt
      781 tcgctgcatt aaccatgctg tggtgggggc cagaatcgca gtggcaattc cttcaaagca
      841 aatagcgtga aaaatacgct caagtaacga tctgcgttga atcgcattat gttgcataac
      901 ttacctttct tacagtttcg accgcgttat ggagttgtga gatttttatc gatttatgtg
      961 atatataaaa gatagatacc atcgaaaaaa ttgatagatt atgcgttact cacttgaagc
     1021 gttaagtaca tttgttgaag cggtttcctg tggctccttt tctgctgcgg cacgaagatt
     1081 acgcaaaagt cagtccgcag tcagtacgtc aattgccaat cttgaggtcg atcttggtat
     1141 atcattattc gatcgctctt cgcgagaacc tgtgctgaca gaggaaggaa agaaagtatt
     1201 gagttacgtt catgcgattt tgtcagccag tgaacgtctt gaggaggtgg cggtttcgct
     1261 tgccggagag actgaagctc gtctgacgtt tgtgctttcc gataccctac accctgactt
     1321 attagaagac atcatggtgc agtttgatag tcagtttcca catactgagt tagaggtttt
     1381 aattggtgag gatgtcgatg tcatcgatct tttgcaaaaa gaacgagcgc agatcggctt
     1441 aattgagtct cgggaacact accccaccga tattggctca acccgtttac cgatgcaagc
     1501 gtggatgggc atttatgttt cggcgtcaca tccactgtct gcagaaaaaa caatccagcg
     1561 ggaacaactt cattcatggc gagaacttcg tcttaatact tatatcgata accgggagat
     1621 gattgcccgt ggaccggttt ggtctgcacc taactatttg ttgttactca gtatggccgt
     1681 acagggattt ggctggtgct ctctgccctg tgctctggtt gaggagtttg caacagaaaa
     1741 accgctggtt cagctcgacg tacctggctg gccaaaggca atttctatcg atctgctttg
     1801 gaataaaaaa tcgccaccag gcgcggctgg aaattggctt aagcatcatt tacagcatat
     1861 caatgcccca aatagtcgaa cttagattag ttaaaatatg gaatcctgaa ataatctatg
     1921 ttgatatgtg gagccatctt ccaggctccc acctaaatca tgttagcagt tccatttttt
     1981 tgatgatttc cgcatgagca tcatcgttat ccccagcgac aaattgaata caaaaccagt
     2041 gctaccgcca ttgcaattcc taccgttgcg aatgcttcag gccagctcat tgattcacct
     2101 cctgcggcgg ttctggtagt ggcatccaat tgattacatc gcattcaggg atgctgatat
     2161 catcaccaag ccacccttga ccttcagacc agcattgcgc gtaatacccg tattctgtgt
     2221 tcactacgca ccactgcgcg tcgttcggca ttcgctcact acagcttatc caactatccg
     2281 gagttaccgg aagcgagaac ggcagcacat ctctgtgaac aagtttttgc tgtgacaggt
     2341 tatccagaac tttctgtact gctgcatcac cgaatacacc aagcgcatct gccataactc
     2401 ctacaacctg ataagcctca gcgcataccg tggataaacc atccggaatt accggagagt
     2461 tgccgggttc tttaatgtgc aagcgaggct caccatcttt tggctcaggc cactggcgct
     2521 ccatgttgat cttcaattta tcttccatag caacgttaat ttcagcatcg ctgatgccag
     2581 cacggcgttg tgcatcccac aacagaaact gcatatcagc ccactcgcta agatcgtctg
     2641 gttcggctgc ggcttccagt gcctcttttg agaggtgttt cagtggacca atggggccaa
     2701 cgcagccaaa tgtggagtca gaccatttgg catgctcgtg gcgaatctgt tcgcgttcca
     2761 gtgatgccag tgcaattcgt gccagttcca tttgttcgcc acgagtaagc ccgttttcaa
     2821 gcggattttt aatgaacaat tcaatacgtt ctttggtaat agtggtcatg tgttaatcct
     2881 caaaacttta tgcccgggcg caaaagtacg tgttttgtct ttgcttattc gccacccatc
     2941 tttacgggcc tcttttgcac aaccagacca tgacgtaccg atatactcac cgaagtctgg
     3001 cactggatat acaccttccg tacactggcg acaatcacaa tagagatgca tggtataact
     3061 tgcggcaata gccatatcag tctcctttga tgcgaatacc agcagcgcgg atagatttcc
     3121 attgttccca catgcggttg aagtctgttc ctgataaggt cgcgttacgg tatccagttt
     3181 cggtgcgcaa tccttcaagc gtcgatttgg aaatacaaat tgaatgcatt acccatgatt
     3241 caaacaaatc acgctcccga gcttcgcaac tttgtttact catctcttca atgcattgtt
     3301 tagcttctaa atcagcaatt ctgtcaacca cggcatcgac agcatctgaa aagccgaacc
     3361 agttgctcca ctccggcctg ttaccggttg ctgcaaagta catatcatct aaagcagact
     3421 cagcatggtc acgctcgtta atgagttgct cttcgctttt ctccagttct gcaatacgct
     3481 tgttttggga ttcccgttca tccagcagcg ccagcacaat cgatggtgtt accagctcat
     3541 ggaaaaggtc cgcatcaaat ccccagtcgt catgcattgc ctgctctgcc gcttcacgca
     3601 gtgcctgaga gttaatttcg ctcacttcga acctctctgt ttactgataa gctccagatc
     3661 ttcctggcaa cttgcacaag tccgacaacc ctgaacggcc agacgtcttc gttcatctat
     3721 cggatcgcca cactcacaac aatgagtggc agatatagcc tggtggttca ggcggcgcat
     3781 ttttattgct gtgttgcgct gtaattcttc aatttctgat gctgaatcaa tgatgtctgc
     3841 catctttcat taatccctga attgttggtt aatacgcttg agggtgaatg cgaataataa
     3901 aaaaggagcc tgtagctcca tgatgatttt gtttttcatg ctcaccgttc cttaaagacg
     3961 ccgtttaaca tgccgatcgc caggcttaaa tgagtcggtg tgaatcccat cagcgttacc
     4021 gtttcgcggt gcttcttcag tacgctacgg caaatgtcat cgacgttttt atccggaaac
     4081 tgctgtctgg cttttttgat ttcataatta gcctgacggg caatactgcg aagggcgttt
     4141 tcttgctgag gtgtcattga acaagtccca tgtcggcaag cataagcaca cagaatatga
     4201 agaccgctgc cagaaaaatg cattcagtgg ttgtcatacc tggtctctct catctgcttc
     4261 tgctttcgcc accatcattt ccagcttttg tgaaagggat gcggctaacg tatgaaattc
     4321 ttcgtctgtt tctactggta ttggcacaaa cctgactcca atttgagcga ggctatgtgc
     4381 catctcgata ctcgttctta actcaacggg agatgctttg tgcatacagc tccccgttta
     4441 ttatttatct cctcagccag ccgctgggct ttcaggggat ttcggataac agaaaggccg
     4501 ggaaataccc agcctcgctt tgtaacggag tagacgaaag tgatcgcgcc tacccggata
     4561 ttatcgtgag gatgcttcat cgccattgct ccccaaatac aaaaccaatt tcagccagtg
     4621 cctcgtccat tttttcgatg aactccggca ccatctcgtc aaaactcgcc atgtactttt
     4681 catcccgctc aaccacgaca taatgcagtc cttcacgctt catacgcggg tcatagttgg
     4741 caaagtacca ggcatctttt cgcgtcaccc acatgctgta ctgcacctgg gccatgtaag
     4801 ccgactttat ggcctcgaaa ccaccgagcc ggaacttcat gaaatcccgg gaggtaaacg
     4861 ggcatttcag ctcaaggcca ttgccgtcac tgcataaacc atcgggagag caggcggtgc
     4921 gcatactttc gtcgcgatag atgatcgggg attcagtaac attcacgcct gaagtgaatt
     4981 caaacagggt tctggcgtcg ttctcgtact gttttcccca ggccagcgcc ttagcattaa
     5041 cttccggagc cacaccggtg caaacctcag ccagcagggt gtggaagtag gacattttca
     5101 tgtcaggcca cttctttcct gagcggggct ttgctatcac gttgtgaact tctgaagcgg
     5161 tgatgacgcc gagccgtaat ttgtgccatg catcatcccc ctgttcgaca gctctcacgt
     5221 cgatcccggt acgctgcagg ataatgtccg gtgtcatgct gccaccttct gctcagtggc
     5281 tttctgtttc aggaatccaa gagctttcac tgcttcggct tgtgtcagtt ctgacgatgc
     5341 gcgaatgtcg cggcgaaata tctgggaaca gagcggcaat aagtcgtcat cccatgtttt
     5401 atccagggcg atcagcagag tgttaatctc ctgcatggtt tcatcgttaa ccggagtgat
     5461 gtcgcgttcc ggctgacgtt ccgcagtgta tgcagtattt tcgacaatgc gctcggcttc
     5521 atccttgtca tagataccag caaatccgaa ggccagacgg gcacactgaa tcatggcttt
     5581 atgccgcaac attcgtttgg gatgcgactg ccacggtccg gtgatttctc tgccttcgcg
     5641 ggttttgaat ggttcgcggc ggcattcatc catccattcg gtaacgcaga tcggatgatt
     5701 acggtccttg cggtaaatcc ggcatgtaca ggattcattg tcctgctcaa agtccatgcc
     5761 atcaaactgc tggttttcat tgatgatgcg ggaccagcca tcaacgccca ccaccggaac
     5821 gatgccgttc tgcttatcag ggaaggcgta aatttctttc gtccacggat taaggccgta
     5881 ctggttggcg acgatcaaca atgcgatgaa ctgcgcatcg ctggcatcac ctttaaatgc
     5941 cgtctggcga agagtggtga tcagttcctg tgggtcgaca gaatccatgc cgacacgttc
     6001 agccagcttc cctgccagcg ttgcgagtgc agtactcatt cgttttatac ctctgaatca
     6061 atatcaacct ggtggtgagc aatggtttca accatgtacc ggatgtgttc tgccatgcgc
     6121 tcctgaaact caacatcgtc atcaaacgca cgggtaatgg cttttttgct ggccccgtga
     6181 cgttgcaaat gatcgatgca tagcgattca aacaggtgct ggggcaggcc tttttccatg
     6241 tcgtctgcca gttctgcctc tttctcttca cgggcgatct gctggtagtg acgcgcccag
     6301 ctctgagcct caagacgatc ctgaatgtaa taagcgttca tggctgaact cctgaaaatg
     6361 gctgtgaaaa tatcgcccgc gaaatgccgg gctgattagg aaaacaggaa aggagattag
     6421 tgattcaggc cgttaccgcg tccgtcgaga aaaacttcca cgagcaaatc acgggtataa
     6481 gtgcgttcga tgccgcgatg cagatatact ggcaactttc ccggcgagcc tggctaaccg
     6541 ggaccagaac gaactgaacg aaatccgccg ccagtgggtt ctggctttcc gggaaaacgg
     6601 gatcaccaca atggaacagg tgagcgccgg agtgcgtgtc gcccgtcggc agaatcgacc
     6661 attcctgcca tcacccgggc agtttgttgc ctggtgccgg gaagaagcat ccgttaccgc
     6721 cggactgcca aacgccagcg agctggttga tatggtttac gagtattgcc ggaagcgcgg
     6781 gctgtatctg gatgcagagt cttatccgtg gaaatcaaac gcgcactact ggctggttac
     6841 caacctgtat cagaacatgc gggccaatgc gttgactgac gcggaattac ggcgcaaggc
     6901 tgccgatgaa ctgtcctgta tgaccgcacg aattaaccgt ggtgaggcta tacctgaacc
     6961 agtaaaacaa cttcctgtca tgggcggtag acctctaaat cgtgcacagg ctctggcgaa
     7021 gatcgcagaa atcaaagcta agttcgggct gaaaggagcg atgaaataga gctaaagtat
     7081 tagcttaaaa ataaagtata cctagcgaat ataatctagg actagttgta acgcccgaaa
     7141 tgattgattg cgtgagcaac aaaatttttc ttccattgtt gtgaaatcat ttagccataa
     7201 agtttat
//
LOCUS       DAZFJL010000183         6988 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZFJL010000183 DAZFJL010000000
VERSION     DAZFJL010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.185"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            121..1107
                     /locus_tag="ACHJ8C_004290"
     CDS             121..1107
                     /locus_tag="ACHJ8C_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HFO5554047.1"
                     /translation="MSELIATAASSINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENDNAPYIGVNCAAIPENMLEATLFGYDKGAFTGAIASVPGKMELANNGTL
                     LLDEIGDMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQDVTAGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYATVLVKNITLSESTRRALLNYRW
                     PGNVRQLENAIQRGMILNRDGVIYPDALGLPETDIADHSELQWPVQPAVHIAETSDLG
                     QHGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKQGIEVFS"
     gene            1122..1463
                     /locus_tag="ACHJ8C_004291"
     CDS             1122..1463
                     /locus_tag="ACHJ8C_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO5554048.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPAMTFSSTDPDVSF
                     NRIMYGALGHVDQFQQVAEQQQTAVDTGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            1468..3114
                     /gene="fliF"
                     /locus_tag="ACHJ8C_004292"
     CDS             1468..3114
                     /gene="fliF"
                     /locus_tag="ACHJ8C_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO5554049.1"
                     /translation="MNAQIKKLTQAFPAFRLRLVDNKRWALMAGVGLAVAATAIIVSV
                     LWTGNRGYVSLYGRQENLPVSQIVTVLDGEKLTYRIDPQSGQILVPEDALSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSSQFVQNVRYKRSLEGELAQSIMSLDAVESARVHLA
                     LNEESSFVVSDEPQNSASVVVRLHYGAKLNMDQVNAIVHLVSGSIPGLQASKVSVVDQ
                     AGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTRASVANVLDSLVGTGNYRVSVMPDL
                     DLSTIDETQEHYGDAPKINREESVLDSDTNQVAMGVPGSLSNRPPIAANQMSNGTEEN
                     RSPEALSKHSESKRDYSYDRSVQHIQHPDFAVKRLNVAVVLNQNAPALKNWKPEQTTQ
                     LTALLNNAAGIDAQRGDNLTLSLLNFVPQAVPVEPVIPLWKDDSVLAWVRLIGCGLLA
                     LLLLFFVVRPVMKRLTAVRAPVITPEPEAVSEPWIAMPEEERKNVDLPSLPGDDSLPS
                     QSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            3092..4102
                     /locus_tag="ACHJ8C_004293"
     CDS             3092..4102
                     /locus_tag="ACHJ8C_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO5554050.1"
                     /translation="MSELTTNKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVVRLS
                     ENMARLSGVKTSMARKVINNFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNRFQGNQQVI
                     LDQMRERDEEVLGQIQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQAQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAEQT
                     AE"
     gene            4106..4816
                     /gene="fliH"
                     /locus_tag="ACHJ8C_004294"
     CDS             4106..4816
                     /gene="fliH"
                     /locus_tag="ACHJ8C_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO5554051.1"
                     /translation="MTIETLRGRYRLHRFPPRQRAVTGEPLTASISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGCKQFVEAAQPL
                     EAITGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPT
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALQETLTPESQGE"
     gene            4809..6149
                     /gene="fliI"
                     /locus_tag="ACHJ8C_004295"
     CDS             4809..6149
                     /gene="fliI"
                     /locus_tag="ACHJ8C_004295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO5554052.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESWL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTCRSVDEPLDVGVKAINGLLTIGKG
                     QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSI
                     VVVAPADESPLMRLKATELCHSIAAWFRDRGHHVLLLVDSLTRYAMAQREIALSLGEP
                     PATKGYPPSAFGMIPKLVESAGNSESAGSMTAIYTVLAEGDDQQDPIVDCARAVLDGH
                     IVLTRKLAEAGHYPAIDIGQSISRCMNQVTSPEHQQSARAMKQNYAAYMEIKPLIPLG
                     GYVAGADASVDKAVKMFPAIERFLRQEMHEPASLELVQSRLQILFPNGKKAEGQ"
     gene            6152..6586
                     /gene="fliJ"
                     /locus_tag="ACHJ8C_004296"
     CDS             6152..6586
                     /gene="fliJ"
                     /locus_tag="ACHJ8C_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO5554053.1"
                     /translation="MRQIIDTLAQLQRLRDKSVKDKTVELAKQKQICAGYDNNIKALG
                     YLVEKTSAGAAASVESLKNVSGYKGTLRKVIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQHESATAKAQKAVDDIAVQCWLRHKLAE"
     gene            6589..6984
                     /locus_tag="ACHJ8C_004297"
     CDS             6589..6984
                     /locus_tag="ACHJ8C_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HFO5554054.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARTLGERLLVGVSSDALNIAK
                     KGRAPVYPQDDRMAIIAGLACVDGVFLEESLAQKAEYLWGYSADILVMGDDWAGKFDD
                     FSCICEVVYFPRTPSVSTTGIIEVIRGHF"
BASE COUNT         1662 a         1901 c         2077 g         1348 t
ORIGIN      
        1 ataactagtt gtttataaac aaataatatt tttattatcg tttaattcga acttgttaat
       61 cccaccggtg atacgcttca gaacatcctg ttttctgtcg ctactgatat cgttcaccct
      121 atgtctgaac ttattgcaac tgccgcttcc agcattaacg cttttactct ggcaaagcgt
      181 gtcgccgcct ttaacgtacc ggtgctcatt cagggcgaaa ccggcgcggg gaaagagtgc
      241 gtggcgaaat atattcacac tgtagccttt ggtgaaaatg ataacgcgcc ctatatcggc
      301 gtgaactgtg cggcgatccc ggaaaatatg ctggaagcga ccttatttgg ctatgacaaa
      361 ggcgcattta ccggcgcaat tgccagcgtg ccaggaaaaa tggagctggc gaataacggc
      421 accttattgc tcgatgaaat tggcgatatg ccgctggcat tacaggccaa aatattacgc
      481 gtattgcagg aacagcaggt tgagcgatta ggcagcaacc gacaaatcaa actcaatttt
      541 cgcctgattg cctgcaccaa taaaaatctg gaacaggatg tgactgctgg acgttttcgt
      601 gaagatcttt attatcgtct ggcggttatt cctataacca tgccgccact gcgtgaacgt
      661 ctgaacgata ttattccgct ggcagagtcg tttattaaaa aatatgccac ggtgctggtg
      721 aaaaatatca ccctttcaga atctacccgc cgagcgctac ttaattaccg ctggcccgga
      781 aatgttcgcc aactggaaaa cgccattcag cgaggaatga tcttaaaccg cgacggcgtg
      841 atttacccgg atgccttagg cctgccggaa actgacattg ccgaccacag cgaactgcaa
      901 tggcctgttc agcccgccgt ccacattgcc gaaaccagcg atttaggcca gcacggacga
      961 agtgcgcagt atcaatatat cgccgacctg atgcgtaaat atcagggcaa ccgcagcaaa
     1021 atcgccgacc tgttaggcat taccccgcgc gcactgcgct atcggctggc ctccatgcgc
     1081 aagcaaggta tcgaagtttt ctcctgaccc ggaagttaaa aatgagcatt accacaatca
     1141 acagcaccat gcaggcgcaa atcatgcagg acgtgcagcg gatgcaggcc aacgcccagg
     1201 cgccggtgct acccgccatg accttcagtt ccacagaccc ggacgtgtcg tttaaccgca
     1261 ttatgtacgg cgcgcttggt cacgttgatc agttccagca ggtggcggag cagcagcaaa
     1321 cggctgtcga cacgggcaaa agtgatgacc tggcaggtgc gatgatcgcc agccagcagg
     1381 cgtcgctctc tttctccgcg ctggtgcagg tgcgcaataa aatcgccacc gggtttaacg
     1441 acctgatgag tatgtcgatc taaccctatg aatgcacaaa taaagaagtt aacccaggcg
     1501 tttccggcgt ttcgactgcg tctcgtagat aacaaacgct gggcgttgat ggcgggagtg
     1561 gggcttgccg tggcggcgac ggcgattatc gtcagcgtgc tgtggaccgg taatcgcggc
     1621 tacgtttcgc tctacggacg ccaggaaaat ctgcccgtgt cgcagattgt cacggtgctg
     1681 gacggcgaaa agctcaccta ccgcatcgac ccgcagagcg ggcagattct ggtgccggaa
     1741 gatgcactgt cgaaaacgcg aatgaccctt gccgcgaaag gcgtgcaggc gatattgccc
     1801 agcggctacg agctgatgga taaagacgaa gtgctcggca gcagtcagtt cgtgcagaac
     1861 gtgcgctata aacgcagtct cgaaggggag ctggcgcaga gcatcatgtc gctggacgcg
     1921 gtggaaagcg cgcgggtgca tttggcgctc aacgaagaaa gttcgtttgt ggtcagcgat
     1981 gagccgcaaa acagcgcctc ggtggtggtg cgcttgcatt acggcgcgaa gctgaatatg
     2041 gaccaggtga acgccattgt gcatctggtg tccggcagta ttccggggct acaggccagt
     2101 aaagtcagcg tggtcgatca ggcgggaaat ctgctgaccg acggcattgg cgcaggcgag
     2161 gcggtttctg ccgcgacccg taaacgcgat cagatcctca aagacatcca ggacaaaacc
     2221 cgtgccagcg tggcgaacgt gctggattcg ctggttggca cggggaatta ccgcgtcagt
     2281 gtgatgccgg atctcgacct cagcaccatc gacgaaaccc aggaacacta cggtgacgcg
     2341 ccgaaaatca accgcgaaga gagcgtgctg gacagcgaca ccaatcaggt ggcgatgggc
     2401 gtgcctggtt cgctcagtaa ccgtccgccg attgcagcga atcagatgag caacggtacg
     2461 gaagaaaacc gctcgccgga agcgctttcc aaacacagcg aaagtaagcg cgactactct
     2521 tatgaccgca gcgtccagca tattcagcat cccgacttcg cggtgaaacg cctcaacgtg
     2581 gcggtggtac tcaatcaaaa cgctccggcg ctgaaaaact ggaagccgga gcagaccaca
     2641 cagcttaccg cgctgctgaa taacgccgct gggatcgacg cccagcgtgg tgacaatctc
     2701 accttatcgc tactcaactt tgtgccgcag gcggtgcctg tcgaaccggt tattccactg
     2761 tggaaagatg acagcgtact ggcctgggtg cggctgattg gctgcggcct gctggcgctg
     2821 ttgctgctgt ttttcgtggt gcgcccggta atgaaacgcc tgacggcggt gcgtgcgccg
     2881 gttatcacac cagaaccgga agctgtcagc gaaccgtgga ttgccatgcc ggaagaggag
     2941 cgcaaaaacg tcgacctgcc gtcgctgcct ggtgatgaca gcctgccgtc ccagagttcc
     3001 ggcctcgaag taaaactcga gttcctgcaa aaactggcca tgagcgacac cgatcgcgta
     3061 gccgaagttc tcagacaatg gatcaccagc aatgagcgaa ttgacaacaa ataagagcaa
     3121 cagctacctg gaacaggcgg cgatcctgct gctgtgtctg ggcgaagagg cggcggcgac
     3181 ggtgatgcaa aaactcagcc gcgaagaagt ggtgcgcctg agcgaaaaca tggcgcgact
     3241 ttccggcgtc aaaaccagca tggcgcgcaa ggtgatcaat aacttctttg atgagttccg
     3301 cgaacagagc gggatcaacg gcgcatcgcg ctccatgttg cagggcattc ttaacaaggc
     3361 gctgggcacc gagatcgcca gcagcgtgat caacggcatc tatggcgatg agatccgctc
     3421 gcggatggcg cgcctgcaat gggtagaacc acgccagctg gcgatgctga tttccgaaga
     3481 acatctgcaa ttgcaggcgg tgttcctcgc gtttctgacg ccggaaatct cggcagcggt
     3541 gctctcttat ctcaacgaat cggtgcaaaa cgaaattctc taccgggtgg cgaaactcaa
     3601 cgacgtcaac cgcgacgtgg tggatgagct ggacagactt attgaacgtg gcctgtcggt
     3661 actttcggaa catggctcga aggtaaaagg catcaaacag gcggcggata tcgtcaaccg
     3721 cttccagggc aatcagcagg tgatcctcga ccagatgcgc gaacgcgacg aagaggtgtt
     3781 ggggcagatc caggatgaga tgtacgactt ctttatcctc agccgccaga acgaagaagt
     3841 gcgtcggcgt ctgcttgacg aagtgccgat ggaagactgg gcggtggcgc tgaaaggcac
     3901 cgaagccctg ctgcgccgct cgatttacgc ggtaatgcca aaacgtcagg cgcagcaact
     3961 ggaagcgatc accgcgcgtc tggggccggt gccggtcagc cgtatcgaac agatccgccg
     4021 cgaaatcatg ggcattgccc gtgaactgga agaggcgggc gaaattcagc tacagctctt
     4081 tgcggaacag acggcggagt aattcatgac cattgaaaca ctgcgcgggc gctatcgcct
     4141 gcatcgattt ccgccgcgcc agcgtgcggt gaccggagaa ccgctcaccg ccagcattag
     4201 cgccgccgat taccagcgtc agttgatgga cggttttcag gaagggctgc aaaaagggtt
     4261 tgctcaggga atgaccgaag gtcaggagca gggctttagc gaaggtcatc aacaaggttt
     4321 tgccgagggg cgacgtcagg gctacaccga aggcagcctc gccgggcagc aggaaggatg
     4381 caaacagttt gttgaggcgg cgcagccgct ggaagccatc accggtaaag tgaacgactt
     4441 cctcgcgcat attgaacgta aacagcggga agatttgctg caactggtgg agaaagtcac
     4501 ccgtcaggtg atccgctgcg aactggcgct gcaaccgacg cagttactgg cgctggtgga
     4561 agaggcgctg gcggccttcc cgacgatgcc ggagaccctg caagtgatgc tgagtacaga
     4621 agagtttaac cgcctgcgcg acgcggtgcc ggaaaaagtc agcgaatggg ggctgacgcc
     4681 ttcgccggat ctgccgccag gcgaatgccg agtgattacc gacaaatctg aactggatat
     4741 cggctgtgag catcgccttg aacaatgcat gaccgcgctg caagagacgc tgacgccgga
     4801 gtcacagggt gagtgatttt tctgctttcg ataacgccct gcgctcgctg gagtcgatcc
     4861 cgctggcccg cgtcgccggg cggctggtgc gcctgaacgg cattctgctg gagagcgtcg
     4921 gctgcccgct aatgaccggg caactgtgcc gcatcgaaag cgccaatcac acgctgatcg
     4981 acgcgcaggc agtaggcttt aatcgcgaca tcacctactt aatgccattt aaacagccgg
     5041 tcggcctgat ggcaggcgca cgcgtcttcc cggaagaaaa agcccacgac atcctgattg
     5101 gcgaaagctg gttaggccgc gtggttaacg gtctcggcga accgctggat ggcaaaggcc
     5161 gcctgaacgg caacgacctt ctgccgccgc tgccaccctc ggtcaatccc ttaacctgcc
     5221 gttcagtgga cgaaccgctg gacgtgggcg tgaaagcgat taacggcctg ctgaccatcg
     5281 gcaaaggcca gcgtgtcggg ctgatggcgg gcagcggcgt cgggaaaagc gtgctgctgg
     5341 ggatgatcac ccgccagacc aaagccgaca ttgtagtggt cgggttgatt ggcgaacgcg
     5401 ggcgcgaagt gaaagagttt attgaccatt cgctgggcgc ggacgggctg gcaaagtcta
     5461 tcgttgttgt cgcgcctgcc gatgaatcgc cgctgatgcg cctgaaagcc accgaactgt
     5521 gccactccat cgccgcctgg tttcgcgacc gcgggcatca cgttttactg ctggtggatt
     5581 ccctgacccg ctacgccatg gctcagcggg aaattgctct ttcactcggc gaaccgcccg
     5641 ccaccaaagg ctatccgcca tcggcattcg gcatgatccc caaactggtc gagagcgcag
     5701 gcaacagcga aagcgcagga tcgatgaccg ccatctacac cgtgctggcg gaaggcgacg
     5761 accagcaaga tccaatagtc gactgcgccc gcgcagtgct ggacggacat atcgtattaa
     5821 cccgcaaact ggcggaagcg gggcactatc cggctattga tatcggccag tcgatcagcc
     5881 gctgcatgaa ccaggtcacc agcccggaac atcagcaatc cgcccgtgcg atgaaacaaa
     5941 actacgccgc ctatatggag atcaaaccgc tgatcccgct cgggggctac gtggcaggtg
     6001 ccgacgccag cgtcgacaaa gcggtgaaaa tgttcccggc tatcgagcgg tttttgcgcc
     6061 aggagatgca cgaacccgct tccctcgaac tggtgcaaag ccgcctgcaa atcctctttc
     6121 ccaacggcaa aaaggcagaa gggcaataac catgcgacaa atcatcgaca ctctggcgca
     6181 gttgcagcgc ctgcgggata aatcggtcaa agacaaaacc gtcgaactgg caaagcaaaa
     6241 acagatctgc gcgggctatg acaacaatat caaagcgtta ggctacctgg tggagaaaac
     6301 gtctgcgggc gcggcggcgt cggtggaatc gctgaaaaac gtcagcggct acaaaggtac
     6361 gctgcgcaag gtgatcgcct ggcaggagca ggaaaaaacg ctggctaaca tcaaagccac
     6421 gcgaatgcag aaaaacctga cggcggcggc gtgcgaagag aaagtagtgg ccctgacgct
     6481 ggacgacaaa cgccgcgagc agcatgaaag tgccacggcg aaagcgcaaa aagcggtgga
     6541 tgacatcgcc gttcagtgct ggctacggca caaattagcg gagtagccat gaaaacggtc
     6601 atcaccttcg gcaccttcga tgtgtttcat gtcggtcatc tgcgcctttt acaacgcgcc
     6661 cgcacgctgg gcgaacggct gctggtcggc gtatcgtccg acgccttaaa tatcgccaaa
     6721 aagggcagag caccggttta ccctcaggat gaccgcatgg cgattatcgc cgggctggcg
     6781 tgcgtggatg gcgtatttct ggaggagtcg ctggcgcaga aagcggaata tctgtggggg
     6841 tacagtgcgg atattttggt gatgggtgat gactgggcag ggaagtttga cgatttctcg
     6901 tgtatctgtg aggtggtcta ttttccccgt acgccatcgg tatcgacgac ggggatcatt
     6961 gaggtgatta gggggcattt ctgataat
//
LOCUS       DAZFJL010000184         6851 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZFJL010000184 DAZFJL010000000
VERSION     DAZFJL010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.186"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(45..521)
                     /locus_tag="ACHJ8C_004298"
     CDS             complement(45..521)
                     /locus_tag="ACHJ8C_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO5554055.1"
                     /translation="MKLISNDLSDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPVGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAIFS"
     gene            630..1760
                     /locus_tag="ACHJ8C_004299"
     CDS             630..1760
                     /locus_tag="ACHJ8C_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO5554056.1"
                     /translation="MKRAYKYRFYPTTEQAELLAQTFGCVRFVYNSILRWRTDAYYER
                     KEKIGYLQANARLTALKKEPEYIWLNDVSCVPLQQSLRHQQAAFANFFAGRAAYPAFK
                     SKRHKQVAEFTASAFKHRDGELYIAKSKSPLDVRWSRELPSAPSTVTISRDSAGRYFV
                     SCLCEFEPVSMPVTAKTVGIDVGLKDLFVTDTGFKTDNPRHTAKYAKRLTLLQRRLSR
                     KQKGSRNRIKARLKVARLHAKIADCRMDNLHKLSRKLINENQVVCVESLKVKNMIRNP
                     KLSKAIADAGWSELVRQLQYKGKWAGRSVVAIDQYLPSSKCCSCCGFTMQKMPLNVRK
                     WHCPECGADHDRDINAARNIKAAGLAVLAHGEPVNPESQHAA"
     gene            complement(1871..3160)
                     /gene="bioA"
                     /locus_tag="ACHJ8C_004300"
     CDS             complement(1871..3160)
                     /gene="bioA"
                     /locus_tag="ACHJ8C_004300"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO5554057.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEAQLREQLAPACDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3247..4287
                     /gene="bioB"
                     /locus_tag="ACHJ8C_004301"
     CDS             3247..4287
                     /gene="bioB"
                     /locus_tag="ACHJ8C_004301"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO5554058.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4284..5438
                     /gene="bioF"
                     /locus_tag="ACHJ8C_004302"
     CDS             4284..5438
                     /gene="bioF"
                     /locus_tag="ACHJ8C_004302"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO5554059.1"
                     /translation="MSWQEKIDAALDARRVADALRRRYPVAQGAGRWLVADDCQYLNF
                     SSNDYLGLSHHPQIIRAWQQGADQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSDTVADYLVQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDTRREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5425..6180
                     /gene="bioC"
                     /locus_tag="ACHJ8C_004303"
     CDS             5425..6180
                     /gene="bioC"
                     /locus_tag="ACHJ8C_004303"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO5554060.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6173..6850
                     /gene="bioD"
                     /locus_tag="ACHJ8C_004304"
     CDS             6173..6850
                     /gene="bioD"
                     /locus_tag="ACHJ8C_004304"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO5554061.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRISSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESSVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAILHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
BASE COUNT         1628 a         1832 c         1895 g         1496 t
ORIGIN      
        1 cctttaagtg ataccagatg gcattgcgcc atctggcaga gtgattaact aaatatcgca
       61 gtaatcgagg cactcgccag agagtgaaaa tgaacgttaa acccgaccat cgcgccgctg
      121 gcaccttcat cgacatcaat acgttctaca tccagcgcgt gaacggtaaa aatgtagcga
      181 tgggtttcgc ctttcggcgg cgctgcgcca tcgtatccgg ttttaccaaa gtcggtacgc
      241 gtctgtaaaa cgccgtctgg cattgctacc agaccagagc caaacccttg cggtaatacg
      301 cgggtatcag cgggtaaatt aacaactacc cagtgccacc agccggagcc agttggcgca
      361 tccgggtcgt agcaggtgac aacaaaactt ttcgttccca caggaacatc atcccacgcc
      421 agatgcggtg aaatattatc gccatcgtaa cccatgccgt taaagacatg acgatgcggc
      481 agcttatcgc catcgctcag atcgttactg atgagtttca ttagaatgcc tccgggaaac
      541 ctcggccttc agaccgggga ggaaaggagg cggttttccg actaactgta ctttgcataa
      601 tcacattttc ctctttagta tgtgaacaca tgaaacgcgc atataaatac cggttttacc
      661 cgacaactga gcaggctgag cttttagctc agacgtttgg ctgtgtgcgc ttcgtctaca
      721 attccatcct tcgttggcgt accgatgcgt actacgagcg aaaagaaaag atcggttatc
      781 tacaggccaa cgctcgcctt acggcgctca aaaaagagcc tgaatacata tggctgaatg
      841 atgtttcctg cgttcccctc cagcagtcgt tgcgccacca acaagccgcc tttgctaact
      901 tctttgccgg acgagctgca tatccggctt tcaaaagcaa acggcacaaa caggtggctg
      961 agttcactgc cagcgcgttt aaacaccgtg acggcgagtt gtatatagca aagagcaagt
     1021 cgccgctgga tgttcgctgg agtcgagaat taccatctgc gccgtcaacc gttaccattt
     1081 ccagagatag cgctggcagg tactttgttt cctgcctgtg tgagtttgaa cctgtatcaa
     1141 tgcctgttac cgctaaaacg gtcggcattg atgtgggctt aaaagattta ttcgtcaccg
     1201 ataccggatt caaaaccgac aatccccgcc acaccgctaa atatgcgaag cgattaacgc
     1261 tgctacagcg acgtttaagc aggaagcaaa aaggctcaag aaaccgtatt aaagcccgct
     1321 taaaggtcgc ccgactccac gcgaaaatcg ccgattgccg gatggacaat ctgcacaagt
     1381 tgtcccgcaa actgattaac gaaaaccaag ttgtttgcgt cgaatccctc aaggtgaaaa
     1441 acatgatccg caacccgaag ctgtctaaag caatagctga cgcaggctgg agcgaacttg
     1501 ttcgccagct ccagtacaaa ggcaaatggg ccgggcggtc agtggtcgcc attgaccagt
     1561 atttaccgtc ctcaaaatgc tgtagttgct gcggtttcac catgcaaaaa atgcctctta
     1621 atgttcgtaa atggcactgc cctgaatgcg gcgcagacca tgatcgcgac attaacgcgg
     1681 cacgtaatat taaagctgcc gggctggcag tgttagccca cggagagcct gtaaaccctg
     1741 aatcgcagca cgcggcttag gttcggctcg ttgaagtggg aatccccgtc attcagggcg
     1801 gggagccgtc aagagccctc ctttcttgtt tgcagaaagt gtagccagaa accctcacgc
     1861 tgacttcccg ttattggcaa aaaaatgttt catcctgcac cgcacggtta accgctgcgg
     1921 tcagacgctg caactgttgc gggagaataa tatagggcgg catcaggtaa atcagtttgc
     1981 caaaaggccg gatccagaca ccctgttcga caaagaattt ttgcagcgct gccatattca
     2041 ccggacgagt ggtttcgacc acgccgattg cccccagtac gcgcacatcg gcaaccattt
     2101 cggcgtcaca ggcgggggca agttgctcgc gtagctgcgc ttcaatagcc gccacctgat
     2161 gctgccagtc gccagattca agaatcgcca ggctggcgtt tgctgctgcg caggccagcg
     2221 gattgcccat aaaagttggc ccatgcataa agcagccggc ttcgccgtta ctgatggttt
     2281 ccgcaacctc gcgcgtggtg agcgtggcgg aaagggtcat tgtgccgccg gttaaggctt
     2341 taccgagaca caaaatatcc ggcgcgattt ctgcatgttc acaggcaaac agtttgccgg
     2401 tacgaccaaa tccggtggcg atctcgtcgg caatcagcaa gataccttcg cgatcgcaca
     2461 ttttgcggat tcgttttaac cattccggat ggtacatgcg catcccgcct gcgccctgga
     2521 caatcggttc aatgatcacc gccgcgattt catgacgatg cgctgccatc aggcgggcaa
     2581 agcccaccat atcacgctca tcccattcgc catccatgcg gctttgcggg gcgggagcaa
     2641 acaggttttc tggcagatag cctttccaca ggctgtgcat tgagttgtcc ggatcgcaca
     2701 ccgacatcgc gccaaaggta tcgccatgat aaccattgcg gaaggttaga aaacgctggc
     2761 gcgcttcgcc tttggcctgc cagtactgca acgccatttt catcgccact tctaccgcta
     2821 cggagccgga gtccgcgaga aaaacgcact ccagcggttg cggcgtcatt gccaccaatt
     2881 tgcggcacag ctcaatggcg ggcgcatggg tgataccgcc aaacatcaca tgcgacatag
     2941 catcaatttg cgacttcatc gccgcattaa gctgcgggtg attgtaaccg tggatcgccg
     3001 cccaccagga cgacataccg tcaaccaggc gtctgccgtc agacaaaatt agctcgcaac
     3061 cttcggcgct tgccaccgga taaaccggca gcggggaggt catggatgtg tatgggtgcc
     3121 agatatggcg ttggtcaaag gcaagatcgt ccgttgtcat aatcgacttg taaaccaaat
     3181 tgaaaagatt taggtttacg agtctacacc gaattaacaa caaaaaacac gttttggaga
     3241 agccccatgg ctcaccgccc acgctggaca ttgtcgcaag tcacagaatt atttgaaaaa
     3301 ccgttgctgg atctgctgtt tgaagcgcag caggtgcatc gtcagcattt cgatcctcgt
     3361 caggtgcagg tcagcacttt gctgtcgatt aagaccggag cttgtccgga agattgcaaa
     3421 tattgcccgc aaagctcgcg ctacaaaacc gggctggaag ccgagcggtt gatggaagtt
     3481 gaacaggtgc tggagtcggc gcgcaaagcg aaagcggcgg gatcgacgcg tttctgtatg
     3541 ggcgcggcgt ggaagaatcc ccacgaacgc gatatgccgt acctggaaca aatggtgcag
     3601 ggggtaaaag cgatggggct ggaggcgtgt atgacgctgg gcacgttgag tgaatctcag
     3661 gcgcagcgcc tggcgaatgc cgggctggat tactacaacc acaacctcga cacctcgccg
     3721 gagttttacg gcaatatcat caccacgcgt acctatcagg aacgcctcga tacgctggaa
     3781 aaagtgcgcg acgccgggat caaagtctgc tcaggtggca ttgtgggttt aggcgaaaca
     3841 gtaaaagatc gcgccggatt attgctgcaa ctggcaaacc tgccgacgcc gccggaaagc
     3901 gtgccaatca acatgctggt gaaggtgaaa ggcacaccgc tggcggataa cgatgatgtc
     3961 gatgcctttg attttattcg caccattgca gttgcgcgga tcatgatgcc gacctcttac
     4021 gtgcgccttt ctgccggacg cgagcagatg aacgaacaga ctcaggcgat gtgctttatg
     4081 gctggcgcaa actcgatttt ctacggttgc aaactgctga ccacgccgaa tccggaagaa
     4141 gataaagacc tgcaactgtt ccgcaaactg gggctgaatc cgcaacaaac cgccgttctg
     4201 gcgggcgata acgaacaaca gcagcgtctg gagcaggcac taatgacccc ggacaccgac
     4261 gaatattaca acgcggcagc actatgagct ggcaggagaa aatcgacgcg gcgcttgatg
     4321 cgcggcgtgt tgctgatgcc ttgcgtcgcc gttatccggt ggcacaagga gccggacgct
     4381 ggctggtggc agatgattgc cagtatctga acttttccag taacgattat ttaggtttaa
     4441 gccatcatcc gcaaattatc cgcgcctggc agcagggcgc ggatcaattt ggcgtcggta
     4501 gcggcggctc cgggcatgtc agcggttata gcgtggcgca tcaggcgctg gaagaagaac
     4561 tggccgagtg gctgggctat tcacgggcgc tgctgtttat ctccggtttt gcggctaatc
     4621 aggcagtcat tgccgcgatg atggcgaaag aggaccgtat tgttgctgac cggcttagcc
     4681 atgcctcatt gctggaggct gcaagtttaa gcccgtcgcc gcttcgccgt tttgctcata
     4741 acgatgtcac tcatctggcg cgactgcttg cttccccctg tccggggcag caactggtag
     4801 tgacagaagg tgtgttcagc atggacggcg atagtgcgcc actggaggaa atccagcagg
     4861 taacgcaaca gcacgatggc tggttgatgg tcgatgacgc ccacggtacg ggcgttatcg
     4921 gggaacaggg gcgtggcagc tgctggctgc aaaaggtaaa accagaattg ctggtggtga
     4981 cttttggcaa aggatttggc gtcagcgggg cagcggtgct ttgctccgat accgtggcgg
     5041 attatctggt gcaatttgcc cgccatctta tttacagcac cagtatgccg cccgctcagg
     5101 cgcaggcatt acgtgcatcg ctggcggtca ttcgcagtga tgagggtgat acacggcgcg
     5161 aaaaactggc ggcactcatt acgcgttttc gtgccggagt acaggatttg ccgtttacgc
     5221 ttgctgattc atgcagcgcc atccagccat tgattgttgg tgataacagc cgtgcgttac
     5281 aactggcaga aaaactgcgc cagcaaggct gctgggtcac ggcgattcgc ccgccaaccg
     5341 tacctgctgg tactgcgcga ctgcgcttaa cactaacagc cgcgcatgaa atgcaggata
     5401 tcgaccgtct gctggaggtg ctgcatggca acggttaata aacaagccat tgcagcggca
     5461 tttggtcggg cagccgcgca ctatgagcaa catgcggatc tacagcgcca gagtgctgac
     5521 gtcttactgg caatgcttcc acagcgtaaa tacacccacg tactggacgc gggttgtgga
     5581 cctggctgga tgagccgcca ctggcgggaa cgccacgcgc aggtgactgc gttagatctc
     5641 tcgccgccaa tgcttgttca ggcacgccag aaggatgccg cagaccatta tctggcggga
     5701 gatatcgaat ccctgccgtt agcgactgca accttcgatc ttgcatggag taatctcgcg
     5761 gtacagtggt gcggtaattt atccacggca ctccgcgagc tatacagggt ggtgcgcccc
     5821 ggaggcgtgg tcgcgtttac cacgctggtg cagggatcgt tacccgaact gcatcaggcg
     5881 tggcaggcgg tggacgagcg tccgcatgct aatcgctttt taccgccaga tgaaatcgaa
     5941 cagtcactga acggtgtgca ttatcaacat catattcagc ccatcacgct gtggtttgat
     6001 gatgcgctca gtgctatgcg ttcgctgaaa ggcatcggtg ccacgcatct tcatgaaggg
     6061 cgcgacccac gaatattaac gcgttcgcag ttgcagcgat tgcaactggc atggccgcaa
     6121 caacaggggc gatatcctct gacgtatcat ctttttttgg gagtgattgc tcgtgagtaa
     6181 acgttatttt gtcaccggaa cggataccga agtggggaaa actgtcgcca gttgtgcact
     6241 tttacaagcc gcgaaggcag caggttatcg gacggcaggt tataaaccgg tcgcctctgg
     6301 cagcgaaaag accccggaag gcttacgtaa tagtgacgca ctggcgttac agcgcatcag
     6361 tagcctgcag ctggattacg caacggtaaa tccttatacc ttcgcagaac ccacctcgcc
     6421 gcacatcatc agcgcgcaag agggcaggcc gatagaatca tcggtaatga gcgccggatt
     6481 acgcgcgctt gaacaacagg ctgactgggt gttagtggaa ggtgctggcg gctggtttac
     6541 gccgctttct gacactttca cttttgcgga ttgggtaaca caggaacaac tgccggtgat
     6601 actggtagtt ggtgtgaaac tcggctgtat taatcacgcg atgttgactg cgcaggcaat
     6661 actacacgcc ggactgactc tggcgggttg ggtggcgaac gatgtcacgc ctccgggaaa
     6721 acgtcacgct gaatatatga ccacgctcac ccgcatgatt cccgcgccgc tgctgggcga
     6781 aattccctgg cttgcagaaa atccagaaaa tgcggcaacc ggaaagtaca tcaatcttgc
     6841 cttaatgtag t
//
LOCUS       DAZFJL010000185         6634 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFJL010000185 DAZFJL010000000
VERSION     DAZFJL010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.187"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..520
                     /locus_tag="ACHJ8C_004305"
     CDS             <1..520
                     /locus_tag="ACHJ8C_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO5554062.1"
                     /translation="SGVAFNALADDPNQGSGKITFKGEVIDAPCSIAPGDEDQTINLG
                     EVADTVLKSGQKSLPVDVTIHLQDCILSDGTNTVDKVKITFSSASVDATDSNLLKNTL
                     EGNIGGATDVGVRLVKSDNTNVTLGTPITINFPTTNSYQELNFKARMESLGRTATPGN
                     VQAQANYVLDYK"
     gene            607..3129
                     /locus_tag="ACHJ8C_004306"
     CDS             607..3129
                     /locus_tag="ACHJ8C_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5554063.1"
                     /translation="MNNKNTFSRDKLSHAIKNALSGVVCSLLFVLPVHAVEFNVDMID
                     AEDRENIDISRFEKKGYIPPGRYLVRVQINKNMLPQTLILEWVKADNESGSLLCLTKE
                     NLTNFGLNTEFIELLQNIAGSECLDLSQRQELTTRLDKATMILSLSVPQAWLKYQATN
                     WTPPEFWDTGIAGFILDYNVYASQYAPHHGDSTQNVSSYGTLGFNLGAWRLRSDYQYN
                     QNFADGRPVNRDSEFARTYLFRPIPSWSSKFTMGQYDLSSNLYDTFHFTGASLESDES
                     MLPPDLQGYAPQITGIAQTNAKVTVAQNGRVLYQTTVAPGPFTISDLGQSFQGQLDVT
                     VEEEDGRTSTFQVGSASIPYLTRKGQVRYKTSLGKPTSVGHNDINNPFFWTAEASWGW
                     LNNVSLYGGGMFTADDYQAITTGIGFNLNQFGSLSFDVTGADASLQQQNSGNLRGYSY
                     RFNYAKHFESTGSQITFAGYRFSDKDYVSMSEYLSSRNGDESIDNEKESYVISLNQYF
                     ETLELNSYLNVTRNTYWDSASNTNYSVSVSKNFDIGDFKGISASLAVSRIRWDDDEEN
                     QYYFSFSLPLQQNRNISYSMQRTGSSNTSQMISWYDSSDRNNIWNISASATDDNIRDG
                     EPTLRGSYQHYSPWGRLNINGSVQPNQYNSVTAGWYGSLTATRHGVALHDYSYGDNAR
                     MMVDTDGISGIEINSNRTVTNGLGIAVMPSLSNYTTSMLRVNNNDLPEGVDVENSVIR
                     TTLTQGAIGYAKLNATTGYQIVGVIRQENGRFPPLGVNVTDKATGKDVGLVAEDGFVY
                     LSGIQENSTLHLTWGDNTCEVTPPNQSNISESAIILPCKTVK"
     gene            3145..3885
                     /locus_tag="ACHJ8C_004307"
     CDS             3145..3885
                     /locus_tag="ACHJ8C_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5554064.1"
                     /translation="MKSPLITTGMIYLLGICNFAQATVSPDRTRIIFNASNKSATVRL
                     TNQSKIDPYLAQSWIEDASGKKTRDYISTLPPMERIEPDEQVQIRLMALASLNNLPQD
                     RETLFYYNVREIPPRTKEQNVMQIAMQSRLKLFWRPKAIELKEGQMVPLQKVTITRTA
                     AGLTLNNPTPYHITVGYIGTNGKTLMPGADSIMVAPFTSATQHLSSQPSTFQLGFVAD
                     YGGLEMFKVECNSVQSLCQSSPAKKGKI"
     gene            3882..4931
                     /locus_tag="ACHJ8C_004308"
     CDS             3882..4931
                     /locus_tag="ACHJ8C_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFO5554065.1"
                     /translation="MITLFRLLAILCLFFNVSVYAVNCYQDQRGGNTQIRGTLPSFRI
                     PENAQPGQKIWESDDVNVTVYCDNATGWRADDPAENIYAWIKMPAINSADILNNPYLT
                     FGVTYNGVDYEDSNVAIDTFACLDKFEQYYDGFYHNPVCNGRTLQKNVTFNARFRVYV
                     KFKSRPVVDQTWNFGVVNVLQFDGEGGANLAANNKNLRYIIDGLNNISFLDCSVDIRI
                     FPESQIVNFGKLSANSIATYRPKAAFSVSTIKDVAADCTEQFDVATSFYTTDTLHDDT
                     HLEMGNGLLMRITDQKTQEDIKFNKYKRFTTYIPGQTGAMVTRDYQAELSQKPGETLV
                     YGPFKKDLIVKINYN"
     gene            complement(4985..5638)
                     /gene="ribB"
                     /locus_tag="ACHJ8C_004309"
     CDS             complement(4985..5638)
                     /gene="ribB"
                     /locus_tag="ACHJ8C_004309"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO5554066.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITDDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(5742..5890)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6012..6311
                     /gene="ubiK"
                     /locus_tag="ACHJ8C_004310"
     CDS             6012..6311
                     /gene="ubiK"
                     /locus_tag="ACHJ8C_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO5554067.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARYNPVAELQSPPAIPPVDKAE"
     gene            complement(6346..6546)
                     /gene="glgS"
                     /locus_tag="ACHJ8C_004311"
     CDS             complement(6346..6546)
                     /gene="glgS"
                     /locus_tag="ACHJ8C_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO5554068.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
BASE COUNT         1881 a         1594 c         1462 g         1697 t
ORIGIN      
        1 atctggcgtt gcttttaacg cgcttgctga tgacccaaat cagggttctg gtaaaataac
       61 atttaaaggt gaagttatcg atgcgccttg ttctattgct cctggtgatg aagatcagac
      121 aataaacctc ggtgaagttg ctgataccgt attaaaaagc ggtcagaaat ctctgcctgt
      181 agatgtcacc attcacttgc aggattgtat tttatctgac ggcactaaca ctgttgataa
      241 agtcaaaatc acctttagtt ctgccagtgt tgacgctacc gactccaacc tgcttaaaaa
      301 cactctggaa ggtaacatcg gcggtgcaac tgatgtaggc gtacgtctgg tgaaatcaga
      361 caacaccaac gtgactcttg gcactccaat cactatcaac ttcccgacga ctaactccta
      421 ccaggagttg aactttaaag cccgtatgga atctctggga cgcaccgcga ccccgggtaa
      481 cgtgcaggca caggctaatt acgtactcga ctacaagtaa tcggtagtca atacctacac
      541 aacgccaggc ggtaaagccg atgaaatttc atcggcaact ttgggccttt ttagaaatgg
      601 atttttatga acaataaaaa cacgttttcc cgggataagt tatcccatgc aattaaaaat
      661 gccctgtctg gcgttgtgtg ttccctactc ttcgttttac cagtccacgc cgtagaattc
      721 aacgtcgata tgattgacgc agaagaccgt gagaatatcg acatctctcg ttttgagaaa
      781 aaaggctata tcccccctgg tagatacctc gttcgtgtgc aaataaataa aaatatgttg
      841 ccacaaacgt taatactgga atgggtaaaa gccgataatg aaagtggttc gttactctgc
      901 ttaaccaaag aaaatttgac taatttcggt cttaatacgg aatttattga attattgcaa
      961 aacatagctg gcagcgaatg tctcgattta agccaacgtc aggagttaac gacacgactt
     1021 gataaagcta cgatgatatt atcgctaagt gttccccagg catggttaaa ataccaggca
     1081 acaaactgga cgccaccaga gttttgggat actggtatcg ccgggtttat ccttgattac
     1141 aacgtgtacg ccagccagta tgccccacat cacggagaca gcacccaaaa cgtcagctcc
     1201 tatggtacgt taggctttaa cctcggcgca tggcgcttac gtagcgatta ccaatataat
     1261 cagaattttg ctgatggacg cccggtaaac cgcgacagcg aatttgcgcg aacttatctg
     1321 tttcgcccta tcccctcctg gtcgtcaaaa ttcactatgg gccagtacga cctgagctcc
     1381 aatctttacg ataccttcca ctttactggc gcatcactgg aaagtgatga aagcatgctg
     1441 ccgccagatt tacagggtta tgcgccacaa attaccggca tcgcgcagac caacgcgaaa
     1501 gtaactgtgg cacaaaatgg tcgtgtactt tatcaaacca ctgtcgcgcc aggccctttt
     1561 actatttctg atttggggca atcgtttcag gggcagctgg atgtcacagt ggaagaagaa
     1621 gatggccgca ccagcacctt ccaggttggc tccgcatcca ttccctattt aacccgtaaa
     1681 gggcaagtgc gctataaaac gtcactggga aaaccgacat ccgtcgggca taacgatatc
     1741 aataatccct ttttctggac ggcggaagcc tcctggggct ggctaaacaa tgtgtcgctg
     1801 tatggtggtg gcatgttcac cgctgatgat tatcaggcta tcactaccgg tattggcttt
     1861 aaccttaacc aattcggttc gctttctttt gatgtcaccg gagcagacgc gtctttacag
     1921 caacaaaata gcggcaatct gcgtggttac agctatcgct tcaactatgc aaagcatttc
     1981 gaatcgacag gcagtcagat taccttcgcg ggttatcgct tctcagataa agattacgtg
     2041 tcgatgagtg agtacctcag ctcgcgtaat ggcgatgagt caatcgataa tgaaaaagag
     2101 agttatgtca tttccttgaa ccagtacttt gaaacgctgg aattaaactc ttatctcaac
     2161 gttacacgca atacttattg ggacagcgcc agcaatacca actactccgt atctgtaagc
     2221 aaaaactttg atattggcga tttcaaaggt atatctgcat cgctggcagt aagtcgaatc
     2281 cgctgggatg acgacgaaga gaatcaatat tacttctctt tctctctacc tttacaacaa
     2341 aaccgcaaca tctcctacag tatgcagcga acgggaagca gtaatacttc gcagatgatt
     2401 tcctggtacg attcatcaga tcgcaacaat atctggaata tttcagcgtc ggcaacggac
     2461 gacaatatac gtgatggcga accaacactg cgcggcagct accagcacta ttcgccgtgg
     2521 ggacgtctga acattaatgg cagtgtacag ccgaatcagt acaattctgt taccgcaggc
     2581 tggtacggtt cacttaccgc tacacgtcat ggtgtcgccc ttcacgatta tagctatggc
     2641 gataacgccc gcatgatggt cgataccgat ggcatctccg gcattgaaat caactctaac
     2701 cgtaccgtta ccaacgggct gggcatcgcc gtgatgcctt cgttatcgaa ctacaccacc
     2761 tccatgttgc gggtgaacaa taacgatctg ccagaaggtg tcgatgtcga aaactcggtt
     2821 attcgcacca cgctcaccca gggtgccatc ggctacgcaa aactgaatgc caccaccggt
     2881 taccaaatcg tcggcgttat tcgtcaggaa aatgggcgct tccctccact aggtgtgaat
     2941 gtcacggata aagcgacagg taaagatgtg ggcctggtcg cggaagacgg cttcgtttat
     3001 ctcagcggta ttcaggaaaa tagtactctg catttaacct ggggtgataa tacctgtgaa
     3061 gtcacgccgc caaaccaaag taacattagc gaaagcgcaa taattttacc ttgtaaaaca
     3121 gtcaaataat tcaggatgaa tataatgaaa tcaccgttaa tcacaacagg aatgatttat
     3181 ttactgggga tctgcaactt tgcccaggcg actgtctccc ccgacagaac acgcatcatt
     3241 tttaatgctt cgaataaaag cgcaacggtt cgcctgacca accaaagtaa aatcgatccc
     3301 tatctggctc agtcgtggat tgaagatgcc agcggtaaaa aaacgcgtga ttacatctcg
     3361 acattgccgc cgatggaacg tattgagcct gatgaacagg tacaaattcg cctgatggcg
     3421 ctagcctcgt taaacaactt accacaggac agagaaacgc tgttctacta taacgtacgg
     3481 gaaattccgc ctcgcactaa agaacagaat gtgatgcaaa tcgccatgca aagtcgcctg
     3541 aaattattct ggcgtccgaa agcaatcgaa ttaaaagaag ggcagatggt gcctttacaa
     3601 aaagtcacga taacacggac cgctgcaggg ctgacactca ataatccaac gccttatcac
     3661 atcaccgtcg gttatatcgg tactaacggc aagacactga tgcccggtgc ggacagtatt
     3721 atggtggcac catttacttc tgcaactcaa cacctaagca gccagccctc taccttccag
     3781 ttaggctttg tggcggacta cggcgggctg gagatgttta aagtcgaatg taattccgtt
     3841 caatccctct gccaaagctc tcctgctaag aaggggaaaa tatgattacg ctttttcgtt
     3901 tactggcgat tctttgcctg ttttttaacg tttcggttta tgctgtaaat tgctatcagg
     3961 atcaaagagg tggtaatacg caaatacgtg gtactttacc ttccttcaga attccagaaa
     4021 acgcccagcc gggacaaaaa atttgggaaa gcgatgacgt taatgttacg gtatattgtg
     4081 ataacgcgac aggatggaga gctgatgatc ccgcagaaaa tatttatgcc tggattaaaa
     4141 tgccggctat aaatagtgca gacattttga ataacccata cctgacattc ggcgtaacct
     4201 ataatggtgt ggattatgaa gatagcaatg tagcaattga tactttcgcc tgtctggata
     4261 agtttgaaca atattacgat gggttttatc ataatccagt ttgtaatggc agaaccctac
     4321 aaaaaaatgt cacatttaat gcccgttttc gcgtatatgt gaaatttaaa tctcgtcctg
     4381 tagtagatca gacatggaat tttggcgtgg taaatgtcct gcaatttgat ggtgaaggtg
     4441 gtgctaactt agcagcaaat aataaaaacc tgcgttatat cattgatgga ttaaataata
     4501 tttcatttct tgattgtagt gtcgatatcc gtatttttcc agaaagccag atcgttaatt
     4561 tcgggaagct ctctgcaaac tctattgcta cttatcgacc caaagctgca tttagcgtat
     4621 ctactataaa agatgttgct gccgactgta ccgagcaatt tgatgttgcc accagttttt
     4681 atacgacaga tacgttacat gatgatacgc atctggaaat gggtaatggt ttgttaatgc
     4741 gtattactga tcaaaaaaca caagaagaca ttaagtttaa taagtacaaa cgattcacca
     4801 catatattcc aggtcagact ggagcgatgg ttacccgtga ttaccaggct gaactttcac
     4861 aaaaaccggg ggagactctc gtttatgggc ctttcaaaaa agatctcatt gtaaaaataa
     4921 actataactg aacagcaaaa tgccgggaac accccggcat tttcatattt agtatttcac
     4981 gtcattagct ggctttacgc tcatgtgcct gacggtatgc caccagatct tcaatagtca
     5041 cgagcgccat attgtgttta ttggcaaact caatgcactc tggtgcacgc gccatcgtgc
     5101 catcgtcatt agtcagctca cacagcacac cagctggttt aaagcctgcc agcgtcatca
     5161 gatcgatagt cgcttcagta tgaccgccac gcgtcagtac accacctgcc tgcgcgcgaa
     5221 gtgggaaaac gtggcccgga cgattcagat ctgacggttt tgcgccatcg gcaatcgctg
     5281 cgcgaacggt cgtaatacgg tcagcggcag aaacaccggt agtcacacct tcagctgctt
     5341 caatggtcac ggtaaaaccg gtgccatagg cgctggtgtt attttctacc atcattggca
     5401 gatcgagttg tttacggcga tcgtcagtaa tgcacaggca aacaatacca ctcccgtggc
     5461 gaatggtcag cgccatctgc tcaacggtca tggtttctgc cgggaagatc atatcgcctt
     5521 cgttttcacg gtcttcatca tcaagcacca tcacaccgcg tccttcacgc agcgcagcca
     5581 gtgcattttc aacacgttcg aaaggcgtac caaaagagga aagtagcgtc tgattcatgg
     5641 taaaaaaacc tcactaaaat tatggttacc agaatcaggg cagtcttagg agttgcggca
     5701 tatagccaaa ataacgtgag cgggtccatg cccgacagaa tcgttactct ctcccatccg
     5761 gactctaacc gtcggccccg gaattacacc ggatctgctg tcctttgagt tcgcacccaa
     5821 agcgctcgcg ggctttcaac tgagttgatt taccgccggt ggggaatttc gccccgccct
     5881 gagaataagc ggattcacta taacgctaat gattagcgcc agcaacgcat agcttcacat
     5941 aattctggtt tatgacttac ccttatcgca ctacaatggc actcaacacc tatcagtaca
     6001 gggaaactac aatgattgac ccgaaaaaaa ttgagcaaat cgctcgccag gttcacgaat
     6061 caatgcctaa aggaatcagg gagttcgggg aagatgtgga gaaaaaaatc cgccaaaccc
     6121 tacaagcgca gctgacgcgt ctcgatctgg taagccgcga agaattcgac gttcagacgc
     6181 aagtcctgtt acggactcgt gaaaaactgg cgttactgga gcagcgcatc agcgaactgg
     6241 aagcgcgcta taatccggta gcagaacttc aatctcctcc ggctatcccg ccagttgata
     6301 aagcagaata aagcaccaca aaaacgggcc agacggcccg taatatcagt gttctaattc
     6361 aagttctctg gtttgcatca tctgttggca ataccaggca taacgtgcgc aaaaccaggt
     6421 tctatgttct tcatccatgt taccagtaac ggccagaata tcgaccgggc gaccttctgc
     6481 gtgcattctg gcaaaactac gcgcgagaaa atcgaaatta tttaaagaat taagactatg
     6541 atccataagc acatcctccg gttattgcat accttttaag gccatatgct ttttctgatt
     6601 tcagtattcc ccaatcagtg tagtgccttt aaac
//
LOCUS       DAZFJL010000186         6473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFJL010000186 DAZFJL010000000
VERSION     DAZFJL010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.188"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..214
                     /locus_tag="ACHJ8C_004312"
     CDS             <1..214
                     /locus_tag="ACHJ8C_004312"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554069.1"
                     /translation="GYIVKTSSGIETNIGNKCGKNEFGVEFGENVLSFNKFMQLETNR
                     EIISTAKSKCEEWQKILKRYEISNPQ"
     gene            169..735
                     /locus_tag="ACHJ8C_004313"
     CDS             169..735
                     /locus_tag="ACHJ8C_004313"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554070.1"
                     /translation="MAKNIETIRNIKPTIDYLSSTIENSKNSNYSGRLGATEIRFLTK
                     SQTGLVTLSEVETDKNTKTILFAMNEHMRESGEAISEFYIGKVSFSHVLLPENNLRDF
                     FVALKEDLKKIQCIDLQTAPSPEISNVAQIASSIEERIKRLKKLKHEASKFLTKKNLR
                     PIANKIKYSSTADELEFHNFERFLNGLK"
     gene            complement(850..1680)
                     /locus_tag="ACHJ8C_004314"
     CDS             complement(850..1680)
                     /locus_tag="ACHJ8C_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO5554071.1"
                     /translation="MKLEASLKYFSPQGMYIGDDVKGTSPERLTGTDVMAAIGTTSNR
                     ERFGLAAFFGKAGISKTDEQMAVQALARHAMEIAPKNVRKAAGGEFGWCMLVLAQFAF
                     AEYSRSAATSVTCHSCSGTGLTPRKQVIRKVSYPWGKAPYWASRSRAVRPSDWEKWTE
                     VTEVVLVVCDVCRGKGVISARCRCGGKGEVLDRKATSERGVPVFKICERCSGNGFSTV
                     PSTTVYKVVLKRIPELHVRTWTRNWKPFLDSLVDICHQEERKADIAFQNVTSFGDDVN
                     "
     gene            complement(1680..2537)
                     /locus_tag="ACHJ8C_004315"
     CDS             complement(1680..2537)
                     /locus_tag="ACHJ8C_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5554072.1"
                     /translation="MRTDNNEHKALFTIPTAAYSSALANIKPLPEQRRITGHKQTDAY
                     LWVLEVIRLNEPAHLDAAEAALVKIKISPKEAQERYSRYLLANGGDPFQIAFGTIGMD
                     NPAQAIRIARENIKKATSVRATFGSYEAALEDVEAERVIKSSQKFIDDHLWGWTAAEK
                     TAGRIDGIRMNEIDDQRRAYVDGYRDVLPEPHTLSDVVREFVYWDWLYSVRHTATKEQ
                     GDEYGYSEHHESVYDRERYLEKLLMTIKPVTRAEAVEVCRWFLASGKGEYMKDNGEAV
                     ILNLVGECE"
     gene            complement(2537..3505)
                     /locus_tag="ACHJ8C_004316"
     CDS             complement(2537..3505)
                     /locus_tag="ACHJ8C_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFO5554073.1"
                     /translation="MKTAAAAKGRWPEILEHFGLPPITGKNHFKGECPVCGARGKFRI
                     DDRDGAGTWICVCGSGDGMKLVTLTQAKPFNEICTEIDRLIGNDYQRVKIPVTSSATS
                     LRKRVLSKFSKLEALRGTSGAAYLNSRGIFSLPAEAIRFNARQRHNGSVFQSLYSLAT
                     DDKGELCYLHQTLLDGDKKADIGSSAKRLKSLQEDNYLDHARSVAIRMFPVASTLGIA
                     EGIETALSAHQIYNVNTWATINSGFMKKFRVPAGVLHLIIFADRDENSATGLAAACEC
                     AHANLMAKNDLQRVSVYWPDHDDFNNMLMNGDQVRELVFHKKKAVA"
     gene            complement(3507..5165)
                     /locus_tag="ACHJ8C_004317"
     CDS             complement(3507..5165)
                     /locus_tag="ACHJ8C_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO5554074.1"
                     /translation="MQLTITPNFAQERALNMLRRDWKANDTFMVYSPTGSGKTGLAAF
                     IVAGFVSRGMRVLFCVPYTILIGQTANRFVQYGLPGDEIGYIWADHPNYDPDRKIQIA
                     SADTLIRRVFPENIDLLIIDEAHLRKKRILKDIERLRGKGVKVIGLSGTPFSPFLGKY
                     YDRLIKPTTIGELIQRGDLSKYEFYAPTKPDLKGVKTTSSLEYGRDYNETQLAEIMCG
                     STLVGDIVQNWLENGRDLPTIAFCVNVAHANYLTIQFNLAGVNAEVMTADTPVDERQT
                     IIHRFETGATKIIVSVGVLVAGFDSDVRCIIYARPTKSEIRWLQALGRGLRTAPGKAS
                     CLIFDHSGTVHRLGYPDSIEYDDLPGKSDGMEESARRAVEERAEKLPHECPQCHYMKP
                     AGVYVCPKCGHKPLRGEDVDTDTSRKLNKLGKNQHQSTKAEKQSWWSQIKFYQRQRAS
                     LGRPVSDGWCAHTFREKFGEWPDGLSSFPMEISPEVSNYIKHKLIRFAKGRQRTQRTI
                     EKLQATIPLSQERGERSEMPIGTEAWRIMQAKQQLQKNINSLSQ"
     gene            complement(5277..5645)
                     /locus_tag="ACHJ8C_004318"
     CDS             complement(5277..5645)
                     /locus_tag="ACHJ8C_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554075.1"
                     /translation="MSEDLKQNLITLLEEQFIRSDDKVVFDYVMQKKIKSQGYHLQRN
                     FSISISGGRKGFIDCLVTSSDGQQCAIEVDKKSPRNRSLMKLAQLPEGMSGFVLLRDG
                     KHPLRYSENGIDVIRATKFK"
     gene            complement(5895..6188)
                     /locus_tag="ACHJ8C_004319"
     CDS             complement(5895..6188)
                     /locus_tag="ACHJ8C_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554076.1"
                     /translation="MSQNYVQTEMPSRHCQADEEWIQQQLQGLPPSLRRKVALKYAEV
                     YEITFDAEPVSFRKENRARHEANTRLRLFVRNQGRALQGYTTQPPLAGTQSRS"
     gene            complement(6201..>6473)
                     /locus_tag="ACHJ8C_004320"
     CDS             complement(6201..>6473)
                     /locus_tag="ACHJ8C_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5554077.1"
                     /translation="MKGNDYDALRELIAQNAIARNLGVTPQAVNQWFSKRTIPARFVL
                     RVCEVVEWKVTPHGLRPDLYPHPEDAIPDLLRRSHTATNAAVMKNE"
BASE COUNT         1628 a         1702 c         1425 g         1718 t
ORIGIN      
        1 gggttatatt gtaaaaacat ctagtggcat tgaaaccaac atcggaaata aatgtggtaa
       61 aaacgaattt ggtgtggaat ttggtgaaaa cgttcttagt ttcaataaat ttatgcaact
      121 cgaaaccaat cgggaaatta ttagcaccgc aaaaagtaaa tgtgaagagt ggcaaaaaat
      181 attgaaacga tacgaaatat caaacccaca atagattatc tatcttctac catcgaaaat
      241 agtaaaaatt ccaattactc cggaagactt ggtgctacag aaattcgttt tttgaccaag
      301 agccagacag gccttgtaac cctttctgaa gtggaaactg ataagaatac caaaacaatt
      361 ctgttcgcaa tgaatgaaca catgcgcgag tctggagagg caataagtga attctacata
      421 ggaaaagtat catttagtca tgttctttta ccagaaaaca atcttcgtga tttttttgtt
      481 gcgctcaaag aagatctaaa aaaaattcag tgtattgatc tacaaacagc cccaagccca
      541 gaaatttcta atgttgccca aattgccagt tcaatcgaag aacgaatcaa gcgattaaaa
      601 aaactgaaac atgaagccag taaattccta actaaaaaaa atctgcgtcc catcgcaaac
      661 aaaataaaat actcatcgac agcggatgaa cttgaattcc ataatttcga acgcttttta
      721 aacgggctga aatgataaaa gccccgaaag gggcttctat caaaatcatt tacgcattat
      781 tccgcatgct taaaagaata catgacagat tcggacaaaa tcaagtgcaa tgtcgcaaaa
      841 accctaagct tagttcacat catcaccaaa acttgtcaca ttctgaaaag caatatcggc
      901 cttacgctct tcctggtggc aaatgtcaac cagagaatca agaaacggct tccaattacg
      961 cgtccatgtc ctgacatgta gctcgggtat ccgcttcagt acaactttat atactgtggt
     1021 agacggtacc gtggaaaatc catttccgct gcaacgttcg cagattttaa acactggcac
     1081 accacgctcg cttgtggctt tgcggtccag tacctctccc tttccaccac aacgacacct
     1141 ggcacttatc actcccttcc ctctacatac atcgcaaacg actagtacaa cttccgttac
     1201 ctctgtccat ttctcccaat ctgacggtcg aacagcacgg gagcgactgg cccaatatgg
     1261 tgctttaccc catgggtatg aaaccttacg aatgacctgc ttacggggtg ttagtccggt
     1321 accactgcaa ctatggcatg ttacgctggt ggccgccgat cgggaatact cagcaaaagc
     1381 aaactgcgcc agtaccagca tacaccagcc aaattcacca ccagctgctt tacgcacatt
     1441 cttcggtgca atttccatcg cgtgacgcgc cagcgcctgg actgccatct gctcatcagt
     1501 cttgctgata ccggccttcc cgaagaaggc cgccaggcca aaccgctcac gattgctggt
     1561 ggtaccaata gccgccataa catcggtgcc tgtaagacgt tccggagagg ttcctttcac
     1621 atcgtcgccg atatacattc cctgaggact gaagtatttt agcgatgcct caagcttcat
     1681 tattcacact ccccaaccag attaagaata accgcctcgc cattgtcttt catatattcg
     1741 ccctttccgc ttgccaaaaa ccaacgacac acctccacgg cttcagcccg tgtcaccggt
     1801 ttgatggtca tcagcaattt ttcaaggtag cgctcgcggt catataccga ttcgtggtgc
     1861 tcagagtaac catactcatc gccctgttct ttagttgcag tgtggcgaac actgtagagc
     1921 cagtcccagt aaacaaactc acgaactacg tctgacaatg tatgaggctc tggcagtaca
     1981 tcacgatagc catcaacata tgcacgacgc tgatcatcaa tttcattcat acggatgcca
     2041 tcaattctgc ccgctgtctt ctcggctgca gtccagcccc agagatgatc gtcgataaat
     2101 ttctgggaag acttgatcac tcgctcggct tccacatctt cgagtgctgc ttcatagcta
     2161 ccaaacgtag ccctgactga tgttgctttt ttgatattct cccgggcgat cctgattgcc
     2221 tgtgccgggt tatccatgcc gatggtaccg aaagcaatct ggaaaggatc accaccattc
     2281 gccagcagat aacgcgaata gcgttcctgg gcctcttttg gggaaatttt aattttcacc
     2341 agcgcagcct cagcagcatc cagatgtgcg ggttcgttca gacggataac ctccagcacc
     2401 caaagataag catcagtctg cttatgcccg gtgattctcc gttgctctgg caggggcttg
     2461 atgtttgcga gggcggaact gtacgctgcc gtcgggatgg tgaatagtgc tttatgttcg
     2521 ttgttatcag tacgcattac gcaaccgcct ttttcttatg gaaaaccagc tctcgaacct
     2581 gatcaccgtt catgagcata ttgttgaaat catcgtgatc cggccagtac acgctcacgc
     2641 gctgcaggtc attctttgcc atcagattgg catgagcaca ttcgcaagcc gcagccagcc
     2701 cggtggcgct gttctcgtca cggtcggcaa aaataatcag gtgcagaaca ccagctggta
     2761 cgcggaactt tttcataaag ccgctgttaa tggttgccca ggtgttcacg ttataaatct
     2821 ggtgcgctga cagcgctgtt tcgatgcctt cggcgatacc cagagtgcta gcaacaggaa
     2881 acatgcggat agctacagaa cgagcgtgat ccaaatagtt atcttcctgc agggatttga
     2941 ggcgctttgc actgctaccg atatctgctt ttttatcacc atcaagcaga gtctggtgca
     3001 gatagcacaa ctccccttta tcgtccgtag caagtgaata aagagactgg aacacactcc
     3061 cgttgtgtct ctgcctggca ttgaaccgga tcgcctcagc aggaagactg aatattccac
     3121 gagaattaag atacgctgcg ccggatgtac cacgcagtgc ctccagtttt gaaaacttgc
     3181 tcaatacccg tttgcgtaag ctggtggcgc tgctggttac cgggatttta acccgttggt
     3241 aatcattacc gatcaggcgg tctatttcgg tacaaatctc gttaaatggc ttcgcctgtg
     3301 tcagggtgac aagtttcata ccatcgccac taccacatac acagatccac gttcctgcac
     3361 cgtcgcggtc atcaattcgg aacttgccac gtgcaccgca taccgggcat tcacccttga
     3421 agtgattttt tcctgttatc ggcggcaagc cgaagtgctc taatatttca ggccagcggc
     3481 ctttcgctgc agctgctgtt ttcatcttac tgactcaaac tgtttatatt tttctggagt
     3541 tgttgctttg cttgcatgat gcgccaagcc tcagtgccta ttggcatctc gcttcgctca
     3601 cctcgttcct gagacagagg aatcgttgcc tgcaattttt ctatagttct ctgcgtccgc
     3661 tgacggcctt tggcaaaccg aatcagcttg tgtttgatgt agttacttac ttctgggctt
     3721 atttccatcg ggaaactgct caacccatca ggccattcac cgaatttctc cctgaaagtg
     3781 tgagcacacc atccatcact gactggacgc cccagcgaag cacgctggcg ctgataaaat
     3841 ttgatctgac tccaccagga ctgtttctct gccttcgtcg actgatgctg atttttaccc
     3901 agcttattaa gtttgcggct agtgtcagta tcaacgtctt cacctcgcag cggcttgtgt
     3961 ccacatttcg ggcaaacata gacgccagct ggcttcatgt agtggcattg agggcattca
     4021 tgtggcagtt tttcggcccg ttcctcaact gcgcggcgcg cgctttcctc catgccatca
     4081 gacttaccgg gaagatcgtc gtactcgatt gaatccggat aacccaaacg gtgcacggtg
     4141 ccgctgtgat cgaagataag gcaggacgct ttacccggtg cggtgcgcag cccacgcccg
     4201 agtgcctgca accagcgaat ttcgcttttt gttggcctgg cgtagatgat gcaacgaacg
     4261 tcactatcga atccggctac cagaacgccc acactaacga tgattttcgt tgcaccggtt
     4321 tcaaagcggt gaatgatggt ctggcgctca tctaccggag tgtcggcggt cattacctca
     4381 gcgttaacac ccgccaggtt aaactggatt gtcagataat tggcgtgagc tacgttgacg
     4441 cagaaagcga tggtaggtag atcccgacca ttctccagcc agttctgtac gatgtcgccc
     4501 accagcgtag agccgcacat gatttcagcc agctgtgttt cgttgtaatc gcggccgtac
     4561 tcaagcgaag atgtggtttt aacacctttc agatccggct tagttggcgc gtaaaattcg
     4621 tatttactca gatcgccacg ctggattaac tcgccgatgg tggtcggctt aatcagtcgg
     4681 tcatagtatt tgcccaggaa cggagaaaac ggagtacccg acaggccaat cacctttacg
     4741 cctttgccgc gcagacgttc gatatccttc aggatgcgtt ttttacgcag gtgcgcttcg
     4801 tcgataatca gcagatcgat attttcagga aaaacacgac gaataagcgt gtcagcgctg
     4861 gcaatctgaa ttttccggtc cggatcgtag ttcgggtgat ccgcccagat ataaccgatt
     4921 tcatctccag gtaaaccata ctgcacgaac cgattagccg tctgaccaat caggatggtg
     4981 tacggaacac agaacagaac acgcatacca cggctgacaa aaccagcaac tatgaaggct
     5041 gccagacccg ttttaccgct acctgttggc gagtaaacca tgaaggtgtc gtttgccttc
     5101 cagtcacggc gcaacatgtt tagcgctcgc tcctgtgcaa aattcggcgt gattgtcagc
     5161 tgcattgtgc tgtccccgca gtgatgagat aataattttg tgatgtggtt ttcatggatt
     5221 cctcctcaca tggctggtgg tctccccaaa ggctgccagc ccctttccga atcaactcac
     5281 ttaaatttcg tcgcacgaat aacgtcaatt ccgttctcgc tatatcgaag agggtgctta
     5341 ccatccctga gcaggacaaa gcctgacatc ccctcaggta gctgagccag cttcatcaac
     5401 gaacggttgc ggggagactt cttatcgacc tcaatggcac actgctggcc gtctgatgat
     5461 gtaaccaggc aatcaataaa ccctttacga ccaccgctaa tgctgatgct gaaattgcgt
     5521 tgcagatggt atccctgaga cttgattttt ttctgcatca cataatcgaa aacgactttg
     5581 tcatcggagc gaatgaattg ctcttccagg agagtgatta aattttgctt caggtcttca
     5641 ctcatacttt tttatccatc ttgtactagc tttctggtac aaccgttttt tcaggggtat
     5701 ccccctttag atcgagatct acctaaccta tgtacccgtc tgttggaaaa gcctgttcca
     5761 gtgcttcgca ctaacacacg ggcactcctc cccctcccct cctctcattg aattttgtgc
     5821 gtactcttta actagtacaa aaatgcaatg agatttggga ttcagccacc ggacaccttt
     5881 aagcccggta ccaatcagga gcgcgattgc gttcctgcca ggggcggctg agttgtatac
     5941 ccctgtaaag ctctgccctg atttctcaca aacaagcgga gccttgtgtt tgcttcgtgc
     6001 cttgctctgt tctccttgcg gaatgaaaca ggctcagcgt caaaagtgat ttcgtatacc
     6061 tccgcatatt tcagggcgac cttccgtctc agtgacggag gcagcccctg taactgctgc
     6121 tgaatccact cttcgtctgc ctggcagtgc ctagatggca tctctgtctg aacgtaattc
     6181 tgggacatac aagcccttca tcattcgttt ttcataactg ccgcatttgt ggctgtgtga
     6241 cttctacgta acaaatcagg aattgcatct tcaggatggg gataaagatc tggtcttaac
     6301 ccatgaggag taactttcca ttccacaact tcacatacac gcaagacgaa acgagcagga
     6361 attgttcttt ttgaaaacca ttggttcaca gcttgcggcg taacgcctag atttcgcgct
     6421 atggcatttt gtgcaattag ctcacgaagt gcgtcgtaat catttccttt cat
//
LOCUS       DAZFJL010000187         6442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFJL010000187 DAZFJL010000000
VERSION     DAZFJL010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.189"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..553
                     /gene="fimA"
                     /locus_tag="ACHJ8C_004321"
     CDS             <1..553
                     /gene="fimA"
                     /locus_tag="ACHJ8C_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO5554078.1"
                     /translation="HVGIIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGEVVNA
                     ACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAFLGTT
                     VTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLVLDGATFSAKTDLIDGTNILPFQAR
                     YIALGQSVAGTANADATFKVQYL"
     gene            915..1625
                     /locus_tag="ACHJ8C_004322"
     CDS             915..1625
                     /locus_tag="ACHJ8C_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5554079.1"
                     /translation="MQTTRTPYSVSFMATVLLLLLFACHSTIANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTHLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAVTGDISF
                     QTINDYGALTPRIKATMQ"
     gene            1667..4318
                     /locus_tag="ACHJ8C_004323"
     CDS             1667..4318
                     /locus_tag="ACHJ8C_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5554080.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTFALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMASRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDSKEHSAEKHVPDNSACTPLQDRLADASSEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSTPKFVQASLMHGLKGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADDTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTSEQTLFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTQGGNTSSGTSGYSSLNYRGAY
                     ANTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKATGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPVVSPGTLLNQQTAICR"
     gene            4332..4862
                     /locus_tag="ACHJ8C_004324"
     CDS             4332..4862
                     /locus_tag="ACHJ8C_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5554081.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPVVPFQITLSECSKGTTEVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVSASATFTLEFQ"
     gene            4875..5378
                     /locus_tag="ACHJ8C_004325"
     CDS             4875..5378
                     /locus_tag="ACHJ8C_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5554082.1"
                     /translation="MKRLHKRFLLATFCTLFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSAAGWHNITLSLTDCPIETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            5438..6352
                     /locus_tag="ACHJ8C_004326"
     CDS             5438..6352
                     /locus_tag="ACHJ8C_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5554083.1"
                     /translation="MGKTFSIKVLFGIYLLLIAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGFSLMRNGKTLATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVAAGAVQSVIGVTFIYE"
BASE COUNT         1844 a         1382 c         1431 g         1785 t
ORIGIN      
        1 acatgtcggt attattgtcg tttctgtgtt ggcgatgtcg tctgctgcgg taagcgcagc
       61 cgagggtgat gaatcagtaa cgaccactgt taatggcggt gtaattcatt ttaaaggtga
      121 agtggtaaat gccgcttgtg cgattgattc agaatcaatg aaccaaacgg ttgagctggg
      181 tcaggttcgt tcttctcgcc tggctaaagc gggtgacctc agctccgccg ttggcttcaa
      241 tatcaagctg aatgattgtg ataccaatgt ttccagtaat gcagctgttg cattcctggg
      301 tactactgtc accagtaatg acgatacatt agcgctgcag agttcagcgg caggctctgc
      361 ccaaaatgtc ggtatccaaa ttttggaccg tacaggggag gtattagtac ttgatggtgc
      421 tacttttagc gctaaaactg acttgattga tggcacgaat atactgccgt tccaggctcg
      481 ttatattgct ctcgggcagt cagtagctgg tactgcaaac gctgatgcga ccttcaaagt
      541 tcaatatcta taattcactc ttaaggtgac tatgcgtcat ctgaaattga aacagggtgt
      601 gtgtggcatg gctacgcaca cccgagaaat tcaccaatgt tcatctcatt attaatgaaa
      661 aatggtttgt attttggagc ggtatgaaaa atcacataat atctaaacga tcaataaaca
      721 acagtagtta aacttgctgt tatttaaaat atttacttgg tagcaaggtt ttcctatcaa
      781 aagagaacca acatttatta caaacgcatt aagtctggaa aaccggcgac tcgccaatgc
      841 attagcaata tttaccgatt gtctgatgta acggtttccc cgcttatcct cttaacacat
      901 ggagaatgca tcagatgcaa actacgcgta ccccctactc tgtttccttt atggcgacag
      961 tgttactttt attgctcttt gcttgccata gcaccattgc caacgcagct gtcgcacttg
     1021 gtgcgacgcg ggtaatttat ccggcaaacc agaaacaggt tttattgcct gtcacgaata
     1081 atgatcctgc cagtgtctat ctcattcagt catggataga aaatgcgggt gaccagaaag
     1141 atacccagtt tgtcattacc ccaccgcttt tttccatgca aggtaaaaaa gagaatacgc
     1201 ttcggataat caacgccact aatcatcagt taccaggcga ccgtgaaagc ctgttctggg
     1261 ttaatgttaa agctatccct gctatggaaa aagaccagaa gaatgagaat acattacagc
     1321 tggcaattat tagccgcata aaaatgtttt atcgccccac gcatctggca atggcacctg
     1381 aagaagcgcc tgcaatgctt cggtttcgtc gcagcggtag caaactcact cttattaatc
     1441 caacgcccta cttcattacc gtaacaaata tgaaagcagg aaatagcaat ctcccaaaca
     1501 ccatggtgcc gccaaaaggt gaagtatcgg tggatatccc gcatgctgta accggggata
     1561 tcagttttca gacaatcaat gattacggtg cattaactcc gcgtattaag gcaaccatgc
     1621 agtaagccag tgacctaatt aataataaac tcaatcggga ttaaaaatga cagcctttcg
     1681 tgccgcattt aaggcatatc gtatgcatca ggtgttaata ctgcctcgct ttgcacggct
     1741 tacctttgcc cttggcttag caactgcggt ttttcccgtt gacgcagagt attattttaa
     1801 tccacggttt ttaagcaatg accttgctga atctgtcgat ttatctgcct ttactaaggg
     1861 acgcgaggcc ccacctggta catatcgggt cgacatttat ttgaacgacg aatttatggc
     1921 tagtcgggat attactttta ttgcagatga taataacgca gatctcatcc catgcctgag
     1981 tacagacctt cttgtcagtc ttggcataaa aaaatcagca ttattagaca gcaaggaaca
     2041 ttcggcagaa aaacatgtgc cggacaacag cgcatgcaca ccacttcagg atcgtttggc
     2101 tgacgcatca tctgagtttg atgttggtca gcaacatctg tcactctccg ttccgcaaat
     2161 atatgtcggc agaatggctc gcggctatgt ttctcctgat ctgtgggaag aaggaataaa
     2221 tgccgggcta ctaaactata gttttaatgg taattctatt aataaccgta gtaaccataa
     2281 tgcaggaaaa tccaactatg catatttgaa tttacagagt ggcatcaaca ttggtagttg
     2341 gcgactgcgc gataactcaa cgtggagtta taacagtggg agtagcaatt catctgacag
     2401 caataaatgg cagcatatca atacgtcggc tgaacgtgac attattccgt tacgctcacg
     2461 gttaacagtg ggtgatagtt ataccgatgg cgatattttt gacagtgtga actttcgtgg
     2521 ccttaaaata aattcaacag aagcgatgtt gcccgatagc caacatggtt tcgctccggt
     2581 gattcatggt attgctcgtg gcaccgcaca agtgagtgta aaacagaatg gatacgatgt
     2641 ttatcagact actgtcccac ccggcccttt tactattgat gatatcaact ctgcggccaa
     2701 tggtggtgat ctgcaagtaa ccataaaaga ggcagacggc agtattcaga cattatatgt
     2761 tccttattcg tctgttccgg ttctccaacg tgctggatat acgcgttatg cacttgccat
     2821 gggggaatat cgtagtggaa ataacctgca aagcaccccc aagttcgtac aagcaagctt
     2881 gatgcatggt ctgaaaggaa actggacacc ttatggcgga atgcaaattg cagaagatta
     2941 tcaggccttc aaccttggta ttggtaaaga tttaggactt tttggtgcct tttctttcga
     3001 tattacgcag gccaatacga cacttgcaga tgacacccgt cacagcggac aatcggttaa
     3061 atccgtctac agcaaatcct tctaccagac gggaaccaat atccaggtcg caggatatcg
     3121 ctattctacg caaggttttt ataacttatc cgacagtgca tacagtcgaa tgagtggtta
     3181 caccgtcaag cctcctaccg gagacaccag tgagcagaca ctatttattg attattttaa
     3241 tctgttctac agtaagcgtg gtcaggaaca aataagcatc tctcagcagc ttggaaatta
     3301 tggtacgaca tttttcagtg ccagtcgcca aagttactgg aacacgtcac gcagcgacca
     3361 gcaaatatca tttggattaa atgtgccgtt tggtgatatt acgacttcgc tgaattacag
     3421 ctattccaat aatatatggc aaaacgatcg ggatcattta ctcgctttta cgcttaatgt
     3481 tccctttagt cattggatgc gtacagacag tcagtcggca tttcgtaatt caaacgccag
     3541 ttacagtatg tcaaacgatt tgaaaggcgg catgaccaac ctatcagggg tttatggcac
     3601 tctgctgccg gataataacc tgaattatag cgttcaggtc ggtaacaccc aagggggtaa
     3661 tacatcgtct ggcaccagtg gttacagttc tcttaattat cgtggagctt acgctaatac
     3721 gaatgtcggt tacagtcgga gtggtgacag cagccagatt tattacggaa tgagtggtgg
     3781 gattattgct catgctgatg gcatcacctt tggacagccg ctgggcgaca caatggttct
     3841 ggttaaggct actggcgctg ataatgtcaa aatagagaac cagaccggaa ttcataccga
     3901 ctggcgtggc tatgccatat taccatttgc gacagaatat agagaaaatc gtgtcgctct
     3961 taacgcgaat tcccttgcag ataatgttga actggatgaa accgtagtca ctgtcatccc
     4021 aactcacggt gctattgcca gagcaacatt taatgcacaa atcggcggga aagtattaat
     4081 gacgttgaag tacggtaata aaagcgttcc attcggtgca attgtcactc acggagagaa
     4141 taaaaatggc agcattgtcg cggaaaatgg tcaggtttat ctgactggac ttccacagtc
     4201 agggaaatta caggtttcat ggggcaatga taaaaactca aactgtattg tcgattacaa
     4261 gcttcctgta gtctctcctg gaaccttgct gaaccagcag acagcaatct gtcgctaagg
     4321 aacaatagat tatgaaatac aataacatta ttttcctcgg tttatgtctg gggttaacca
     4381 cttattctgc tttatccgca gatagtgtta ttaaaattag cgggcgcgtc ctcgattatg
     4441 gctgcacagt ctcatcggat tcgcttaatt ttaccgtaga tctccaaaaa aacagtgcca
     4501 gacaatttcc aacgaccggt agcacaagtc cagtcgtccc ttttcagatt acattaagtg
     4561 aatgcagcaa agggacaacg gaagttcggg ttgcatttaa cggtattgag gatgcagaaa
     4621 ataatactct gttgaaacta gatgaaggaa gcaatacggc ctccggtttg ggtatagaaa
     4681 tactggacgg aaatatgcgt ccggtgaaat tgaatgacct tcatgctggg atgcagtgga
     4741 tcccattggt accagaacag aacaatattt tgccttactc cgctcgtttg aagtcaactc
     4801 agaagtccgt caatccagga ctggtgagcg cttcggcaac ctttaccctt gaatttcaat
     4861 aatcagagtc agatatgaaa agacttcaca agaggttcct gttagctacg ttttgcacat
     4921 tattcacagc aactctccag gccgccgatg tcactatcac tgttaatggt cgggtagtcg
     4981 ctaaaccatg cactattcaa accaaagaag ctaacgttaa tctcggggat ctttatacac
     5041 gcaatctgca acaacctggt tctgcagctg gctggcacaa tattactttg tcattaaccg
     5101 attgtccgat tgaaacaagt gcagtgacgg caatcgtgac aggttcaact gacaatacgg
     5161 gttattacaa aaatgaaggt actgccgaaa atattcagat agagctaagg gatgaccagg
     5221 atgcgacgtt aaaaaatggc gatagcaaaa cggttattgt tgatgagatc actcgtaatg
     5281 cacagtttcc acttaaggca agagctatca cggtgaatgg aaacgcaagc cagggtacga
     5341 tcgaggcgct aatcaatgtg atctatacct ggcaataaca tcaatttgat tttattttcc
     5401 tcctttttaa atcaattttt taaccttagg atatatcatg ggtaaaacat tcagtataaa
     5461 ggtcctgttc ggtatttatc ttctattaat tgcaggcaaa gtttttgcct tcagttgtaa
     5521 tgtcgatggc gggtcaagta ttggagcagg tacaacctct gtttatgtta atctcgatcc
     5581 tgtaatacaa ccgggccaga atctggttgt agacttgtct cagcatataa gttgctggaa
     5641 tgattacggc ggctggtacg acactgatca tataaacctg gtacaaggat cagctttcgc
     5701 aggctcccta cagtcatata aaggatcact gtactggaat aatgtgacct accctttccc
     5761 gttgacgaca aataccaacg tattggatat tggtgataag acaccgatgc cattaccgtt
     5821 aaaactctat attaccccag tcggagcggc tggtggagtt gtcataaaag caggggaagt
     5881 tattgcccgt attcatatgt ataaaattgc cacgttagga agcggaaatc cgcgtaattt
     5941 tacgtggaat attatttcaa ataacagtgt tgttatgcca acaggcggtt gcaccgttga
     6001 tagtcgtaat gtcaccgtga atctcccgga ctttcctgga agcgcagaaa taccccttgg
     6061 tgtatattgc tctagcgaac aaaagctctc attttatctt tcgggtgcca ccactgacag
     6121 tgcccgacaa gtgtttgcta acacagcacc agatgcaaca aaagcatcgg gcgtgggttt
     6181 ctctctgatg cgtaatggaa aaacactggc aacaggagaa aatgtctccc tggggacagt
     6241 aaataaaagc aaagtacctc tgggtttaag cgcaacctat ggacaaacag gaaacaaggt
     6301 tgcagccgga gccgtccagt cagtcattgg tgtcactttt atctatgaat aattgtgatt
     6361 ttgacattag tctatgcgct ccattcatga aaattgcgcg gataattatt ttgtgaaggc
     6421 tattatccta cacttgtaag aa
//
LOCUS       DAZFJL010000188         6221 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFJL010000188 DAZFJL010000000
VERSION     DAZFJL010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.190"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..513)
                     /locus_tag="ACHJ8C_004327"
     CDS             complement(<1..513)
                     /locus_tag="ACHJ8C_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5554084.1"
                     /translation="MMSMRKHIAFASMCIGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMIFF
                     RALQGVAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWITDNLD
                     WRWLFYINILP"
     gene            complement(510..1538)
                     /locus_tag="ACHJ8C_004328"
     CDS             complement(510..1538)
                     /locus_tag="ACHJ8C_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO5554085.1"
                     /translation="MIISKKQLIGVVAIGILLAGVVFFIWWVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLES
                     IQATIAMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            1635..2450
                     /locus_tag="ACHJ8C_004329"
     CDS             1635..2450
                     /locus_tag="ACHJ8C_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5554086.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRIWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            2800..2952
                     /locus_tag="ACHJ8C_004330"
                     /pseudo
     CDS             2800..2952
                     /locus_tag="ACHJ8C_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2963..3478
                     /gene="ogt"
                     /locus_tag="ACHJ8C_004331"
     CDS             2963..3478
                     /gene="ogt"
                     /locus_tag="ACHJ8C_004331"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO5554087.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKESYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            3673..4425
                     /gene="fnr"
                     /locus_tag="ACHJ8C_004332"
     CDS             3673..4425
                     /gene="fnr"
                     /locus_tag="ACHJ8C_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO5554088.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            4577..5527
                     /gene="uspE"
                     /locus_tag="ACHJ8C_004333"
     CDS             4577..5527
                     /gene="uspE"
                     /locus_tag="ACHJ8C_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO5554089.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(5577..5834)
                     /gene="ynaJ"
                     /locus_tag="ACHJ8C_004334"
     CDS             complement(5577..5834)
                     /gene="ynaJ"
                     /locus_tag="ACHJ8C_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO5554090.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            6078..>6221
                     /locus_tag="ACHJ8C_004335"
     CDS             6078..>6221
                     /locus_tag="ACHJ8C_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFO5554091.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGDRKRKGFLFHA
                     VQFL"
BASE COUNT         1692 a         1476 c         1407 g         1646 t
ORIGIN      
        1 cggtaatata ttgatataaa atagccatcg ccagtcgagg ttatcagtga tccagccccc
       61 cagagttggc cctagcgtcg gtgacagtga ggccagcgca ctgactaccg cagcagccaa
      121 ccccaactct ttaccctggt aataaataaa cgccgtcgtg aaaactaaag ggatcatgga
      181 tgcgccagca actccctgta aagcacgaaa aaaaatcatg atctgaatat tccatgcaag
      241 tccacaagcg atactcatta aagtaaatat tcccgcggat agagtaaaaa gccaacgagt
      301 ggaaaatacc cgtgacagcc acccagagag cggaataaca ataatctcgg caattaaata
      361 gctggtctga agccaggcca tttcgtcttt acctgcggac agaccaccac ctatttcatt
      421 tagggatgac gatacaattt gaatatcaag ctgagcaata aataatccaa tacacatact
      481 ggcgaaagca atatgtttac gcatagacat catctctcat caacactgac ggtgacggac
      541 agtcccgggc gaagaagttg ctttaattcc tttgcgtcgt caaatgcaat ccgtaccggg
      601 acacgctgaa caattttggt gaaattgcca gtggcatttt ctatcggaat caagctgaaa
      661 ctggctcctg tcgctgggga aaggctttca atgtgaccgt gaaacacttc ccctttgaga
      721 atatcagcac ggatctcagc cttcattcct ggcttcattc ccgctatttg gttttcttta
      781 taatttgcat caacccacaa ctcactgact ggtactagtg aaactaacga tgtgccgcct
      841 tccacccaac tgcctgtatg ggcgctgcgg tttgcgacaa tgccatcaat cggcgcacga
      901 acaagggtat attccagatc cagtagtgcc tgagtcaaag cggcctgaac ttcttcgata
      961 cttgcacgta cgttttcttc ctgagcagat aaaactgcca gccgttgctt ctccaccagg
     1021 aaatcgttcg cggctttgcg ctcctttgct gcgacctgct ggtagtcaaa cctggcgtta
     1081 tcaataattt gttgcgatat tgccgctgat tgtgcgagtt tttcataacg ttcagtatct
     1141 cgcaggcgct tttgctcctc atgttttact gcctgccatg tttcactggc actttgaatt
     1201 atcgactgct gcatcgcaat agtggcttga atgctttcga gattggcttt gctacttttt
     1261 atttttgcct caagcctggc gacatttgca cgataatccc gatcatcgag acgcaggata
     1321 atatcgcctt tttttacact ctgattatcg cgcacctcta tagcagaaat ataaccagaa
     1381 actttacttg ccaccgtggt gatattacca ccaatgtaag catcatccgt cgtctgtata
     1441 aatcttcctt tgcttaccca ccagataaaa aagacaacgc cagccaaaag aataccgatc
     1501 gctaccaccc caataagctg ttttttagaa attatcatca ctctttacac cgtatatatc
     1561 cggaaacttg catacgtcat atttaaaatt taacatggct tatttgatga tttctgacaa
     1621 aatacctact gtaaatgaca atcacactgc aacccaaagc atcaccaggg catcacatta
     1681 tcggactgga ttcggaacat ctgaatggag gcacagttcc ctggcataaa catctctatg
     1741 cccagttgct ttacccggcg gaaggagtcg ttcgtatatg ggctggagaa agcgtctggc
     1801 tagtgcatgc cagcagcgcg ctttggctgc ccccacaaat gccgcataag tttgttgcga
     1861 caggcaacgt acttttaaaa accgtactgg tttcagaagc ggaaagtgaa acgctgggaa
     1921 aagtttgctt catgacgggt atttcacccc tgttaagaga attactaatt gcaattaacc
     1981 aacttccacc gtcgcaaagt accacagata aacaacagtt acgtttttca gccctcgaga
     2041 cactgatact tcaggaaata aaaatggggg tgaaaatgtc acttgaactc ccttggccaa
     2101 atgatgagcg gctacagcag ctttgtgaga atttgctaaa caaccaaggg tatctgccaa
     2161 cactggataa tctagcggat aaaattaacg tcagtagccg tacactcatg cgcttatttg
     2221 ttaaagagac gggattaaca tttcgccact gggttcagca aatgcacgtt atttctgcag
     2281 tgacgcttct tgatgatgga tattcgttga ccaaaattgc ccatcgtctg ggctatgcct
     2341 cagctgaatc gttcggcaat atgtttaagc gtcgaacggg ctactcgccc ggtaaattta
     2401 ctcgtcgatt aacgatgcat gattatgcaa tcacaaggca aatgatttaa cttatatttt
     2461 cgtgaaatct gtcactgaag aaaattggca acggatgggt aaaaccgtta taacactgtc
     2521 accggtcacc aggaccccag gccggattta agacgaggat gcactgctgt gtgtactgta
     2581 gagtctggcg gatgtcgaca ggctctattt ttttattcat tttttagccc tgtaaataac
     2641 cacattctcg ccgggattgg ggaatgagtg acgagatcag tggtgtctgg cggctatcac
     2701 gccacagaat caaataacag atagcctacg gtaaaatttc gctgactgga atttgatggc
     2761 ttgccctcat ttcttcaggc aaaaaaatca aaaaatcgct ttatgcttaa atacttcgac
     2821 aaaccagacg ctaaacctgg tacttacttt ttgttagtat taatcttttc ggtggtatgg
     2881 ctgctgatgt tgctggcgtg gtatcttgtt ggcttgccga caggtccggg tatttaccca
     2941 cgtttgtctt aagagagaac ggatgctgag attacttgaa gaaaaaattg ccacgccact
     3001 gggtccactg tgggtgattt gcgatgagca atttcgcctg cgggcggttg aatgggaaga
     3061 gtacagcgaa cgcatggtgc agctgctgga catccattat cgcaaagaaa gctatgagcg
     3121 catttctgcc accaacccag gtggtttaag cgacaagctt cgtgaatatt ttgccggtaa
     3181 tcttagcatt attgatacgc ttcccactgc cacggggggg acgccatttc agcgcgaagt
     3241 ctggaaaaca ctgcgcacta tcccctgcgg gcaggtaatg cattatggcc aactggctga
     3301 acaattaggc cgccctggcg cggcgcgtgc cgttggtgcg gcaaacggat cgaatcccat
     3361 cagcatcgtc gtaccttgcc atcgggttat tggccgaaac ggcaccatga ccggatatgc
     3421 aggcggagtt cagcgaaaag agtggttatt gcgccacgaa ggttatcttt tgctgtaaac
     3481 attaaacaat ttgtgccagc ttgttcacac ttttatgtaa agtcaccctt aacaacttaa
     3541 gggttttcaa atagatagac atatatttac atctaatatc ggaattctct gctgttaagg
     3601 tttgcttaga cttacttgct ccctaaaaag atgttaaaat tgacaaatat caattacggc
     3661 ttgagcagac ctatgatccc ggaaaagcga attatacggc gcattcagtc tggcggttgt
     3721 gctatccatt gccaggattg cagcatcagc cagctttgca tcccgttcac actcaacgaa
     3781 catgagcttg atcagcttga taatatcatt gagcggaaga agcctattca gaaaggccag
     3841 acgctgttta aggctggaga tgaacttaaa tcgctttatg ccatccgctc cggtacgatt
     3901 aaaagttata ccatcactga gcaaggcgac gagcaaatca ctggtttcca tttagcaggc
     3961 gatctggtgg gatttgatgc catcggcagc ggtcatcacc cgagtttcgc gcaggcgctg
     4021 gaaacctcga tggtatgtga aatcccgttc gaaacgctgg acgatttgtc tggtaaaatg
     4081 ccgaatctgc gtcagcagat gatgcgtctg atgagcggtg aaatcaaagg cgatcaggac
     4141 atgatcctgc tgttgtcgaa gaaaaatgcc gaggaacgtc tggctgcatt catctacaac
     4201 ctgtcccgtc gttttgccca acgcggcttc tcccctcgtg aattccgcct gacgatgact
     4261 cgtggtgata tcggtaacta tctgggcctg acggttgaaa ccatcagccg tctgctgggt
     4321 cgcttccaga aaagcggtat gctggcagtc aaaggtaaat acatcactat cgaaaataac
     4381 gatgcgctgg cccagcttgc tggtcatacg cgtaacgttg cctgattctt ccgcataact
     4441 cactatcctt ctgtcatatc attaaatttt tctgatttat tgatctgaca gaaggttcat
     4501 cactgtttca ttcaccagat atgggttaat cttttaatta caaactgcgt tgacagttgt
     4561 tgtaaggaga ccctgtatgg ctatgtatca gaacatgctc gttgttatcg atcctaacca
     4621 ggacgaccaa ccagcattgc ggcgagctgt ttatttacat caacggattg gtggcaaaat
     4681 taaagccttt ttgccgatct atgacttctc atacgaaatg accaccctgc tctctccgga
     4741 cgaacgtacc gctatgcgtc agggcgtcat cagccagcgt acagcctgga tccacgagca
     4801 ggcaaaatat tatctcaatg ctggcgttcc cattgaaatt aaagtggtct ggcataaccg
     4861 tcctttcgaa gccatcattc aggaagtgat cagcggcggg cacgatttgg tgctaaaaat
     4921 ggcgcaccaa catgaccgtc tggaagcggt gatttttacg ccaacggact ggcatctgtt
     4981 acgcaaatgc ccaagccccg tgtggatggt gaaagaccag ccgtggccgg aagggggtaa
     5041 ggcgctggtg gcggtgaatc tcgccagtga agagccgtac cataatgcgc tcaatgaaaa
     5101 actggtcaaa gagacgatcg aactggcaga acaagtcaac cataccgaag ttcatctggt
     5161 tggcgcttat ccggtaacgc caattaatat cgcgattgaa ctgccggaat ttgacccgag
     5221 cgtttataac gatgccattc gtgggcaaca tttgctggca atgaaagctc tgcggcagaa
     5281 attcggcatt aatgaaaaca tgacgcacgt agaaaaaggt ctgcccgaag aggtgattcc
     5341 tgatttggcg gagcacttac aggcggggat tgtggttctg ggcacggtcg gacgcaccgg
     5401 tatttcagca gcattccttg gcaacacggc ggaacaggtg attgatcatc ttcgctgcga
     5461 cctgctggtc attaaacctg accagtatca gacacccgtt gaactggatg acgaagaaga
     5521 cgattaacga ctcttcaata actgacaagg cccgagttat cgggcctatt ttttacttac
     5581 tgctcttcgc cgccaaggaa ataaattccc aacgggattg ccagcaacac agtaaagacc
     5641 aggctataaa caaaaatcat cgatgactga atcacataca ttgatgtcgt ccactcagac
     5701 agcggaatat tgtactgttc tataacgccg ccgatggtcg cgcgagtcac aaccgacgcc
     5761 gcaataatga aaaccgccaa caaaccaaag agaaatttct tccctttcgc tgacttcagt
     5821 ttcgccataa tcatgatgta atccttgtgc agacaaattt cttgttacct aaacgtaaca
     5881 ataccatgac atttcaccgt tgtctgctct gaaaatctca cctcttagct ggatttacaa
     5941 tcaacgcatt gattagttag ctaatgttgc tacttccttc cctgactatt gatgatctga
     6001 atgaattgtc gcggtaaaat agtcccgcca ggggtttccg gcatgacgac aattatctgg
     6061 aggagcctga taaaacaatg atcgctgaac tgtttacaaa taatgcgctt aatctggtca
     6121 ttattttcgg tagctgcgca gcattgattc tgatgagctt ttggtttcgc cgtggagatc
     6181 gtaaaagaaa aggattttta ttccatgcgg tgcaattttt a
//
LOCUS       DAZFJL010000189         6217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFJL010000189 DAZFJL010000000
VERSION     DAZFJL010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.191"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            129..1037
                     /locus_tag="ACHJ8C_004336"
     CDS             129..1037
                     /locus_tag="ACHJ8C_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFO5554092.1"
                     /translation="MKAPVSFPSLRLQKFIGITVGVLAVITLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRENPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1257..3248)
                     /gene="tkt"
                     /locus_tag="ACHJ8C_004337"
     CDS             complement(1257..3248)
                     /gene="tkt"
                     /locus_tag="ACHJ8C_004337"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO5554093.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQIASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
     gene            3526..4284
                     /gene="loiP"
                     /locus_tag="ACHJ8C_004338"
                     /gene_synonym="yggG"
     CDS             3526..4284
                     /gene="loiP"
                     /locus_tag="ACHJ8C_004338"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO5554094.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSSGLLSSGAEAFQAYSLSDAQ
                     VKKLSDQACQEMDSKATIAPANSEYARRLTTISRALGDNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVLGHEMGHVALGHVKKGMQVALGTNAIRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFTQRQESEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(4375..5295)
                     /gene="speB"
                     /locus_tag="ACHJ8C_004339"
     CDS             complement(4375..5295)
                     /gene="speB"
                     /locus_tag="ACHJ8C_004339"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO5554095.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDIIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5431..6162)
                     /locus_tag="ACHJ8C_004340"
     CDS             complement(5431..6162)
                     /locus_tag="ACHJ8C_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554096.1"
                     /translation="MKKWKVRSALIALAVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFKSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAK
                     NALPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1515 a         1643 c         1617 g         1442 t
ORIGIN      
        1 caaaaatgga ctttttagtt attcatcctt tttggttgct cctttatgga ttctgaattc
       61 atggcaacgg gctgttctcc tttcatctcg tgtgacatat tccctttctt tgctcacgga
      121 tgattcatat gaaagctcca gtttcttttc cttcgcttcg cctacaaaaa tttattggca
      181 ttactgttgg cgtactggca gtcattacgc tgccatttct ttcgtattac accgtaaacg
      241 aaggcgaacg cggcatttta ttgcgctatg gcaagatcgt taaagttgcc gaacccggtc
      301 ttggattcaa aattcccttt atggaaagcg tagagaaaat ttccacgcgt aatcaggctg
      361 tggtctatca gggattacag gcctacagcc gcgaccagca accggcacaa atgacagtat
      421 cagtcagctt ccacattaaa ccttcagaag caggcgcggt ttataccacc tacaacacca
      481 ttgaagcatt aaaagatcgt ctgattgtcc gtcagttgcc aactcaactg gagaacgttt
      541 tcggacaata taccgccatc agcgccgtac aggatcgtac aaaactggtt caggatctgc
      601 aaaatgccat gcgcaaagcc gttgttgggc cagtggtaat tgacggggtc cagattgaaa
      661 atattgattt ttctgacgct tatgaaaagt cgattgaaga ccgaatgaaa gcagaagtcg
      721 ccattgccac gcgtaagcaa aatctggaaa cggagaaaat ccaggcgcaa atagcggtaa
      781 ctcaggccca ggcggaagca gacagtaaac tggcggcagc caaagcagaa gcggaaacta
      841 ttcgcgtaag aggcgcggca gaggctgaaa ctattcgttt aaaaagtgcg gctgaagcag
      901 aagctatccg cctgcgcggt gaagctctgc gggagaatcc cggacttgtt gccctcacca
      961 ctgctgagcg ttgggacggt aaactgcccg ataccatgat ccccggcagc acagttccgt
     1021 ttatctctac taagtaaaaa aagggccgct ttcgcggccc ttgagattga tagcaaagac
     1081 aaaactgcct gatgcgctac gcttatcagg cctacatact tcctgcaata tgttgaattt
     1141 gcaaaatctt gtaggacgga taaggcgttc acgccgcatc cggcataaac aacgcgcact
     1201 ttgccaacaa tctgaagggc cgcatttgcg gcccttctca caaagcatct taccgattac
     1261 agcagcgctt ttgctttcgc aacaacgttg tcaacggtga agccgaactc ttcaaacagc
     1321 tgctctgccg gagcagattc accgaaggtg gtcatgccga cgatagcacc gttcaggcca
     1381 acatacttgt accagtagtc agcaatgcca gcttccaccg caacacgagc ggtaaccgct
     1441 ttcggcagta cggattcacg ataagccgcg tcctgcttgt cgaatgcgtc ggtagacggc
     1501 atggaaacca cgcgcgcttt cacgccttcg gcagtcagtt tttcgtaggc ggcaacagcc
     1561 agttcaactt cagaaccggt cgcgatgaag atcagttccg gctgaccagc acagtctttc
     1621 agcacataac caccgcgcgc gatgtttgcc agctgctctt cagtacgttc ctgctgcgcc
     1681 aggttctgac gggagaggat aagcgcagtc gggccgtcct gacgctcaac gccgtatttc
     1741 cacgctactg cggattcaac ctggtcacac ggacgccatg tagacatgtt cggggtcacg
     1801 cgcagagaag cgatctgttc taccggctgg tgagtcgggc catcttcgcc cagaccgatg
     1861 gagtcgtggg tgtaaaccat cacctgacgc tgtttcatca gcgcagccat acgtacggca
     1921 ttacgtgcgt attccacgaa catcaggaag gttgaggtat acggcaggaa accaccgtgc
     1981 agggagatac cgttagcaat cgcggtcata ccgaactcgc gaacaccgta gtggatgtag
     2041 ttacctgcag catcttcgtt gattgcttta gaaccagacc acagggtcag gttagacgga
     2101 gcaaggtcag cggagccgcc gaggaattcc ggcaacagcg gaccgaacgc ttcgatagca
     2161 ttctgagacg ctttacggct ggcgattttc gccggattag cctgaagttt agcaatgaac
     2221 tctttcgctt tcgcgtcgaa gtcagacggc atttcgcctt tcatacggcg ggtaaattca
     2281 gcggcttcct gcggataagc tttcgcgtaa gcagcgaatt tctcgttcca tgcggactct
     2341 ttcgcctggc ctgcttcttt cgcatcccac tgagcataga tttcagacgg gatttcgaac
     2401 ggcgcgtatt tccagcccag ctgttcgcgg gtcagggcaa tttcagcgtc gcccagcggc
     2461 gcaccgtggg agtcgtgggt accggctttg ttcggggaac cgaaaccgat gatggttttg
     2521 cacatcagca gggacggttt gtcggtcact gcgcgcgctt cttctactgc gcgtttgatg
     2581 gatgccgcgt catgaccgtc gatgtcgcga ataacgtgcc agccgtaagc ttcgaaacgc
     2641 attgcggtgt cgtcagtaaa ccagccttca acgtgaccat cgatagaaat accgttgtca
     2701 tcgtagaacg caatcagttt acccagcttc agcgtacccg ccagagagca aacttcgtgg
     2761 gagatgcctt ccatcatgca gccgtcgccc atgaaggcgt aggtgtagtg gtcgacgatg
     2821 tcgtggcccg gacggttaaa ctgcgccgcc agcgtttttt ctgcaatcgc cataccgact
     2881 gcgttagcaa taccctgacc cagcggaccg gtggtggttt ctacacctgc ggtgtaaccc
     2941 acttccgggt ggcccggagt tttagagtgc agctgacgga agtttttcag ttcttccatc
     3001 ggcagatcgt aaccggtgag gtgcagcagg ctgtagatca gcatggagcc gtggccgttg
     3061 gacagcacga agcggtcacg gtcagcccag gacggattct gcgggttgtg tttcaggaaa
     3121 tcacgccaca ggacttcggc aatgtcagcc atacccatcg gggcacccgg gtgaccggat
     3181 ttggctttct gtactgcgtc catgctcagc gcacgaatag cattggcaag ctctttacgt
     3241 gaggacattt tgactccaga tcggatgatg aagggcacac ccttaacgac ttgacgacag
     3301 cgcgttttgg gctacgccgg aaaatttgcc aacaatttac cgcaagccgc gcgtcatgta
     3361 catggaacat ccttttgccg cttcagaaat ctctggatca tgctcgcatg ttgcgcaatc
     3421 cactcgcccg tccgctgcgc ttttccttat actgagactg agcgtcgatt cacctgcaaa
     3481 cggcgcattt ttagaataat cctgaccttg tgcggaagag aaaacatgaa aattcgcgcc
     3541 ttattggtag caatgagcgt ggctacggtg ctgaccggtt gccagaatat ggactccagt
     3601 ggactgctct catcaggagc ggaagctttt caggcttaca gtttgagtga tgcgcaggtg
     3661 aaaaaactga gcgatcaggc atgccaggag atggacagca aggcgacgat tgcgccagcc
     3721 aatagcgaat atgctagacg tctgacgaca atttctcgcg cgttaggcga taacatcaac
     3781 ggtcagccgg tgaattacaa agtgtatatg gcgaaggatg tgaacgcctt tgcaatggct
     3841 aacggctgta tccgcgtcta tagcgggctg atggatatga tgacggataa cgaagtcgaa
     3901 gcggtgctcg gtcacgaaat ggggcacgtg gcattaggcc atgtgaagaa aggaatgcag
     3961 gtagcacttg gtacaaacgc aattcgcgtt gcggcggcct ctgcaggcgg aattgtcggc
     4021 agtttgtcac aatcacaact gggagacctg ggcgaaaagc tagtcaattc gcaatttacc
     4081 caacgtcagg aatcagaggc cgatgattac tcctacgatt tgctgcgcca acgcggtatc
     4141 agcccggcag gtcttgccac cagctttgaa aaactggcaa aactggaaga aggtcgccaa
     4201 agctcaatgt ttgacgacca tcctgcatcc gccgaacgcg cccagcatat tcgcgatcgt
     4261 atgagcgcgg atggagttaa gtaaagccag gtagtgccga acgtaggtcg gataaggcgt
     4321 tcacgccgca tccgacatta atggcacatc acccgcgcgc ctcttatctg gcgcttactc
     4381 gccctttttc gccgcctgga tatacagcat ctccagcgcc agcgtcgccg cagccagagc
     4441 ggtgatttcc gactgatcgt atgccggtgc cacctccact acgtccatcc caacgatgtt
     4501 gagatctttc aggccgcgta ccagtttaat ggcgcgatcg gaggtcaggc cgccaattac
     4561 cggcgtaccg gttcccggcg cgaaagcagg atccaggcag tcgatatcaa aggtcaggta
     4621 aaccggcata tcacccacaa tctgtttcac ctgggcgata atgtcatcca cgctgcgatc
     4681 gttcacctgg caggcgtcca gcacggtaaa gccgttgtct ttatcaaact cggtacgaat
     4741 accaatctgc acagaatgat tcgggtcgat cagaccttct ttcggcgcgg tatagaacat
     4801 ggtgccgtgg tcaaattcac aaccgttagc gtaggtatcg gtgtgggcat caaagtgtac
     4861 caacgccatt ttgccgaaat gcttcgcatg agcgcgcagc agcggcagcg taacaaagtg
     4921 gtcaccaccg aaggagagca tacgcttacc ggcagccagc agcttctcgg cgtgcgcctg
     4981 cagcttctcg ctcatctcac gggcatcgcc gaaggcatat accagatcgc cgcagtccac
     5041 gacgttcagg cgctcacgca tgtcgaaatt ccacgggaag cgattgtgtt cccaggccag
     5101 attcgtcgaa acctgacgga tcgctgccgg gccgtggcgc ccccccgcac ggccagaagt
     5161 ggccatatcg aacggcacgc cagtaatcac ccagtcagca tcgctgtcat atggctggaa
     5221 gttcatcggc aggcgtaaaa aaccaaaggc gttggaaacc agtgagttat cgtattgatg
     5281 acctaaggtg ctcatggatg gactcctatt acaaagtcga tataaaaaaa cccttccgcg
     5341 tcgttaggcc cgacgaggaa gggttggatt tgtgaaacac aagtttgtgc taattatcgc
     5401 cttaaatggg cgaggcatca agcgaggttg ctatttccga cacggtggcg gcgcatagcc
     5461 attgttatct gccggaaagg cttgtccaga gcacggcggc agcgcgtttt tcgccgggcc
     5521 ttgtaattca aaggcggcaa agccgattac accggtatta cgcacatcgc cttgcgccga
     5581 attggctgcg taggaatcag caacgttggc gaactgaaaa gccgcttcgg tatgtttatc
     5641 tttgcggaat cctttaatcg ctaacgaatc tccggcattc acaatataac cgttgttatt
     5701 cagcgaacct tgtttaccat tcagaacatc caggccatca accgtagcaa cgatctcgta
     5761 gttggtgtta cgactgtaat tacggacata taattgataa ttcattccga ctctggcatg
     5821 gacaatatag cgtggttcat tattggatga atcataaacc cgcgtaattg gtagagattt
     5881 aaaattacca tcgcgaatag catattccag ttcgttaata cgaatgctat aaacgcggtc
     5941 gtagccggaa ggaacattag tcgaataact aataacgatc atctctgccg gatcgcgcca
     6001 ggctcgttca gcagatacgc cctgaaccgt tgagtgcaca tccccacccc aggcggtgcc
     6061 gacattaccc gatgcactgc tattatactg cgcattactg gaacaaccgg ccagcagcac
     6121 ggctaatgct atcaacgcgc tacgtacttt ccattttttc ataaccgttc cctctgcatt
     6181 taattaatgc agcgattgta gggagattat ttaataa
//
LOCUS       DAZFJL010000190         6076 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFJL010000190 DAZFJL010000000
VERSION     DAZFJL010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.192"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            1..3552
                     /locus_tag="ACHJ8C_004341"
     CDS             1..3552
                     /locus_tag="ACHJ8C_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HFO5554097.1"
                     /translation="MTTVNKKLKKTASGAITWSVIVTQILSPVSLSLIPANSFASSGN
                     KDVTQTYASDEHANKVASFAASAGQSLANNNASSFAVNTLSTQATKEVVDWLQQYGNA
                     RIKLNVDDSFSLKDSSFDFLYPWQDTQDYVLFSQTSLHRTDDRNQTNIGLGIRHFTPD
                     NAMLGANVFYDYDLSRSHSRAGFGVEYWRDYFRLGVNTYFGLSDWKNSRDIDDYLERP
                     ANGWDFSAEGWLPAYPQLGASIQFEKYYGKNVGLFGSDNLQENPYAVTGGISYTPVPL
                     IKFSAQHKQGQSNVHDTTFGVEFNYRPGVSLAEQLSSDNVAVMREVQNRRYDFVERNN
                     NIVLEYKKKHALKISLPESVQGEGESIIPVTLTINNASGGIKSVQWNDSAFTAAGGKI
                     SGNGTSWQITLPAYKSGDVNSWNVGATVQDNRGNVSNYAVMNISVNNSGVSTADSSFT
                     LDGDSSPTISADSQSTYPIVLSLKDSNGKALTGLADDIEMSVEFTADSNSARQRETVT
                     APSLGAVEEISAGVYHSVLTAGSQAGTVRVTAKVQGKTFTLSIKQTAATEPDSEVSAV
                     LTAAPAEQVVGYNINLQLAVKDSQGNAITGDNTLSFYALNQAEGVEFGTVTEKDGVYS
                     ATVTSKRAGKITIGVKSDSHDFSGVKKEITFIEDRQQVTFSQFEASQNNALADGKQRN
                     MVTVSLADRFGNVVPGYAVTLSLPAGITQVGGEHAVSTDENGNAIFALISSTPGSYVI
                     TAHAGSQMSTELTVTFASNMTGASLSLTPESSSLISNIPANGKDAAVLDVQLTNTNAS
                     VNGQKIQLITSSEGLSVPTNIVTDSTGHVSVPLTTVKAGEYTVTARVTDGSHSVESGS
                     VKLTFVPDVASAELTMSVSKQEIVADGSENATVNIQLVDANNNAFTGEVDLTITPSTG
                     ASLTSSNLQLDAHGQATTQFTATKSGQYTIQAEYVLDGKRITASQNIDAVTDVKGAVL
                     EITSSASSAVVSDTNNLVFTLLLKSKSGEALSGRALNVKTSGPSKYGALVVDKTTVTT
                     DKNGQATVSVHGRTVGSYKLTATLNELGSDVSAVKSFSLYADEANGVLTLTKDPGYET
                     NDGSPVGIYARFVDHFGNPLSGTVEFSAGSEDSPDSQRVKMEPATVTLHWTGNAASEF
                     STYESGYHWIKAKITNSKNTYEKTIRTYVVKLPEKDS"
     gene            complement(3589..4632)
                     /locus_tag="ACHJ8C_004342"
     CDS             complement(3589..4632)
                     /locus_tag="ACHJ8C_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFO5554098.1"
                     /translation="MKINKILSSATLLYGMSMAMSVGSCATPVQTNLPGYTPGADIIS
                     VSSTRNQVDLIGDVVYSQIKGTRSVRQLHMSVLVPRTNDLKPAIIYYPGGGFMSSEHD
                     KFIEMRMALAEAGFVVAAVEYRTIPDTFPAPVEDGKAAIRYLREHASDYGIDPQRIGV
                     LGDSAGGWLAQMMGTTNGDKTFDKGDFLQQSADVQAVATLYGISDLLNIGEGFPESVQ
                     EVHRSPAVTEALMINGTAFRNFAGAPITASKEKALNASPIGHMKGVKPPFLIMHGSKD
                     TLVSPEQSAKLFRMLKTNGDNAEYVLVEGAEHGDKTWYQPIIINRVVEWFTKNLGAPI
                     KTAPQQQNPNANL"
     gene            4982..5083
                     /locus_tag="ACHJ8C_004343"
     CDS             4982..5083
                     /locus_tag="ACHJ8C_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554099.1"
                     /translation="MCTHNPNSLLPSQMQKCTCVFLHPASDLCGGEA"
     gene            5080..5535
                     /gene="ybcN"
                     /locus_tag="ACHJ8C_004344"
     CDS             5080..5535
                     /gene="ybcN"
                     /locus_tag="ACHJ8C_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO5554100.1"
                     /translation="MNLPQDGIKLHRGNFIAIGQQIQPYLENGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAVNIGCHLTIPQSCEFQLLRDKLEA"
     gene            5535..5705
                     /gene="ninE"
                     /locus_tag="ACHJ8C_004345"
     CDS             5535..5705
                     /gene="ninE"
                     /locus_tag="ACHJ8C_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO5554101.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            5698..5988
                     /gene="ybcO"
                     /locus_tag="ACHJ8C_004346"
     CDS             5698..5988
                     /gene="ybcO"
                     /locus_tag="ACHJ8C_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO5554102.1"
                     /translation="MADLRKAARGRECQVRISGICNGNSETSVLAHIRLAGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            5985..>6076
                     /locus_tag="ACHJ8C_004347"
     CDS             5985..>6076
                     /locus_tag="ACHJ8C_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554103.1"
                     /translation="MNTYHITLPWPLSNNRYYRHNRGRTHISAEG"
BASE COUNT         1698 a         1286 c         1449 g         1643 t
ORIGIN      
        1 atgaccactg tgaataaaaa gttaaaaaaa actgcatctg gcgcgattac atggtcggta
       61 attgttaccc agatattatc tccagtttct ctttctttga tcccagcaaa cagttttgca
      121 tcatctggta ataaagatgt tacgcaaact tatgccagcg atgagcatgc aaataaagtg
      181 gcctcatttg cagcaagtgc aggtcagagc ctagcaaata ataatgcaag tagttttgct
      241 gtaaatactt tatcaactca ggcgacaaaa gaggtcgtcg actggttgca acaatatggc
      301 aatgcgcgca tcaagcttaa tgtcgatgat tctttttcct tgaaggattc atcattcgac
      361 tttttatatc catggcagga tactcaggat tatgtgctat tcagtcagac atcactacat
      421 cgtactgatg accgaaatca gaccaatatt ggtttgggga ttcgtcattt cactcctgat
      481 aacgcaatgt tgggtgcgaa tgttttctat gattacgatt taagtcgcag tcactctcgt
      541 gcaggttttg gggttgagta ctggagagat tatttcaggc ttggtgtaaa tacctatttt
      601 ggtttgtctg actggaagaa cagtcgggat attgatgatt atctggaaag acccgcaaat
      661 ggctgggatt tttctgctga aggatggcta cctgcttatc cgcaattagg ggcatccatt
      721 cagtttgaaa aatattatgg taaaaatgtc gggttatttg gaagcgataa tcttcaggaa
      781 aacccttacg cagttactgg gggaatttct tatacaccag ttcctctgat taagttttct
      841 gcacagcata agcaaggaca gagcaacgtt cacgatacaa cctttggtgt tgagtttaat
      901 tatcgcccgg gcgtttccct tgctgaacag cttagcagtg acaatgttgc tgttatgcga
      961 gaagtccaga accggcgtta tgattttgtt gaacgaaata acaacattgt tctggagtac
     1021 aagaagaagc atgcactgaa aatcagctta ccagagtctg ttcaggggga gggggaatca
     1081 atcatccctg taacactgac aatcaacaat gctagtggtg gtatcaagtc tgtacagtgg
     1141 aatgatagtg cattcactgc ggctggcggt aagatcagtg gaaatggcac atcatggcag
     1201 atcactttac cggcttataa aagcggagat gtaaattcgt ggaatgttgg agccacagtc
     1261 caggataaca gaggcaatgt ttccaactat gccgttatga atatcagcgt taataatagt
     1321 ggtgtctcga cagcggattc atcttttaca ctggatggag attcgtcgcc gacgatctct
     1381 gctgatagcc aatccactta tccgatagta ttaagcctga aagatagtaa cggtaaggca
     1441 ttaaccggac tggctgatga cattgaaatg tcagtggaat ttacagcaga tagcaatagt
     1501 gctcgacagc gtgaaacggt aactgctccg tcattaggcg cggtagagga aatctctgct
     1561 ggggtatatc actctgttct gactgctggt tcgcaggctg gtacggtacg tgtaactgca
     1621 aaagttcagg gaaaaacatt tactctcagt attaagcaaa cagcagcaac agaacctgat
     1681 tctgaggtca gtgctgtttt aaccgctgca ccagcagaac aggtagttgg ttacaatatc
     1741 aatctacaac tggcagtgaa ggattcacaa ggaaatgcaa tcactggtga taacactctg
     1801 agcttttatg ctcttaacca ggcagaagga gtcgagtttg ggactgtaac ggaaaaagac
     1861 ggtgtctata gcgcgacagt aacttcaaaa cgagctggta agattactat cggtgttaaa
     1921 tctgatagtc atgatttctc aggtgttaag aaagaaatca cctttattga ggaccggcag
     1981 caggttacat tctctcagtt tgaagccagc caaaataatg cattggctga tggaaaacag
     2041 agaaatatgg taactgtaag ccttgcggac cgtttcggca atgttgttcc tggttatgct
     2101 gtcacattgt ctttaccagc agggataact caagttggtg gtgaacatgc tgtttcaact
     2161 gacgaaaatg gtaatgctat ttttgcgtta ataagctcca ctccggggtc ttacgtgatt
     2221 acagctcatg ctggttctca gatgtctact gagttaacag tgacctttgc atcaaatatg
     2281 accggagctt cactgtcact gacgcctgaa agcagtagtt taatttccaa tattcctgct
     2341 aatggtaagg atgcagcggt attagatgtg cagctgacaa atacgaatgc gtcggtcaat
     2401 gggcaaaaga ttcagctcat cacctcatct gaaggattat ctgtaccaac aaatatcgtg
     2461 actgattcaa cagggcatgt ttctgttccg cttacaacgg tgaaagctgg tgagtacacg
     2521 gtgactgcac gggtgactga cggttcccat agcgtggaat ctggcagcgt taagttaact
     2581 tttgtgcccg atgttgcttc tgcagaactt actatgagtg tatcaaaaca ggagattgtg
     2641 gccgatggca gtgaaaacgc aacagtaaac attcagttgg ttgatgcgaa taacaatgcg
     2701 tttacaggcg aggtggattt aacgattaca ccatcgacgg gagcatcatt gaccagtagt
     2761 aatctgcaac tggatgctca tggacaggca acaacgcagt tcactgcaac gaaatcaggc
     2821 cagtacacaa ttcaggccga gtatgttctg gatgggaaac gcataaccgc aagccagaat
     2881 attgatgccg taacggatgt gaagggggct gtgttggaga tcacatcatc tgcatcttcg
     2941 gctgtagtga gcgataccaa caatctggta tttacgttac tgttgaaaag taagtcaggc
     3001 gaagcattaa gtggtcgagc attgaatgtt aagacatctg ggccgtctaa atatggtgcg
     3061 ttggtggttg ataaaaccac agtaacgact gataagaacg gtcaggctac ggtaagcgta
     3121 catggtcgaa cggttggttc gtataaatta acggcgacct taaacgaact tggcagtgat
     3181 gttagcgcgg tgaaatcatt ctccctgtac gctgatgagg caaatggagt attgactcta
     3241 acaaaagatc ctggttatga gacaaacgat ggctctccag taggtattta tgctcgattt
     3301 gtcgatcatt ttggaaaccc attatctggt acggttgagt tttctgctgg tagtgaagat
     3361 agtccagact ctcaacgggt caaaatggag cctgctacag taacgttaca ctggacaggt
     3421 aatgcagctt cggaattcag cacctatgaa agtggttatc actggattaa ggccaaaata
     3481 acgaatagca aaaatacata tgagaaaaca ataaggactt atgttgtaaa actacctgaa
     3541 aaggatagtt aacaaaattg aatggggagg gcttagccct ccctcttttt acaggttagc
     3601 gtttgggttt tgttgttggg gagctgtttt tataggcgct cccaggtttt tagtaaacca
     3661 ctcaacgact ctgtttataa taattggctg ataccatgtc ttatcgccat gctcggcccc
     3721 ttctaccagc acgtactcag cgttatcgcc gttcgtcttc aacatcctga atagtttggc
     3781 gctttgctcc ggtgaaacca gagtatcttt gctaccatgc ataataagaa atgggggttt
     3841 tactcctttc atatgtccga ttggactggc gtttagcgct ttttcttttg acgctgtgat
     3901 gggggctccc gcaaaatttc tgaatgcagt gccattgatc attaaggctt cggttacggc
     3961 aggagagcga tgaacctcct gcactgattc agggaacccc tcgccaatat tcaacaagtc
     4021 agaaatccca taaagtgtgg caactgcctg aacatctgct gattgctgaa gaaagtcacc
     4081 tttatcaaag gttttgtcac catttgtagt tcccatcatc tgggcaagcc atccaccggc
     4141 agagtcaccc agaactccga ttctttgagg atcaatccca taatcgctgg catgttctct
     4201 caggtaacgt attgcagctt tcccatcctc aactggtgct ggaaatgtat caggaattgt
     4261 tctgtattct acagcggcca caacaaaacc agcttctgcc agagccattc tcatttcaat
     4321 aaatttgtca tgttcagaag acatgaatcc gccgccggga taataaataa tggctggttt
     4381 taaatcattt gttcgcggga caagaactga catgtgaagc tgtctgacag aacgagttcc
     4441 ttttatctgg gaataaacaa catcaccaat gagatcgacc tggtttctgg tcgatgaaac
     4501 actaatgata tctgcacccg gggtgtaacc aggaagatta gtctggacag gtgttgcaca
     4561 actcccgacc gacatggcca ttgacatacc atacaatagt gttgcagatg ataatatctt
     4621 gttaattttc atgttttaca tgccttgact tatcggatga aattctctta ctgaatttaa
     4681 cggcagttta atagccgtac aactcgtggt agccagggaa atatcccagt cgtggttaag
     4741 gttgtacttg acggctatta tttcaacaat gcttaaagtg agagcttaag gttatgcgta
     4801 tgttttacag tccgagtaga ttctgaccaa tagcttcttt gtataattct gtcagaaata
     4861 aatcttggct tgcatgaagt ttgagacctt atcttgtttg attatgaata atcaattcgc
     4921 cataattgta tcaccggagc ctgaacaact ccggtgactt ctgcgctaaa cggggacgtt
     4981 tatgtgcaca cacaatccaa actctcttct cccttcacag atgcagaaat gcacctgcgt
     5041 ttttttgcat ccagcgtctg acctctgtgg aggtgaagcg tgaacctacc acaagatggc
     5101 atcaaactgc atcgcggtaa cttcatcgct atcggccagc agatccagcc ttatctggag
     5161 aacggaaaat gctttcgcat ggtgcttaaa ccgtggcgtg agaaacgcag tctttcccag
     5221 aatgcactca gccacatgtg gtacagcgaa atcagtgaat acctcatcag cagggggaaa
     5281 tcgttcgcta ccgcagcatg ggtaaaagat gctctcaaac acacatacct cggttatgaa
     5341 accaaggacc tggttgatgt cgtaaccggc gaaatcacta ctatccagtc gttacgccat
     5401 acctccgatc tcgataccgg agagatgtat gtcttcctgt gtaaggttga agcctgggcg
     5461 gtgaatattg gctgccacct gactattccg cagagctgcg agttccagct gctgcgcgac
     5521 aagctggagg cgtaatggct acaccgctta ttcgtgtcat gaacggacac atctacagag
     5581 taccaaatcg tcgtaagcgt aaacctgagc tgaagccatc cgaaatacca acactgctcg
     5641 gatataccgc cagcctagtt gataaaaaat ggttgcgact ggcagcaagg aggaatcatg
     5701 gctgatttga gaaaagccgc gcgtggtcgg gaatgccagg taagaatctc tggcatatgt
     5761 aatggcaact ctgaaacgtc tgtactggca catatccggc tggctggatt gtgcggcacc
     5821 ggtaccaaac cgccagacct gattgccacc attgcatgtt ctgcctgtca cgacgagatc
     5881 gaccgtcgca cgcattttgt tgacgctgag tacgcaaaag aatgcgcgct ggaaggtatg
     5941 gcgagaacgc aggttatctg gctgaaagag ggggtaatca aggcgtgaat acttaccaca
     6001 tcacactacc ctggccgcta agcaataacc gctactaccg gcataatcgc gggcgcacgc
     6061 acatcagcgc agaagg
//
LOCUS       DAZFJL010000191         6070 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZFJL010000191 DAZFJL010000000
VERSION     DAZFJL010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.193"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            124..852
                     /gene="gadW"
                     /locus_tag="ACHJ8C_004348"
     CDS             124..852
                     /gene="gadW"
                     /locus_tag="ACHJ8C_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO5554104.1"
                     /translation="MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFC
                     SLDTRRLDIEELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPIQLVTAIFDSFNDPR
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFSQHSPGTFS"
     gene            complement(1215..4328)
                     /gene="mdtF"
                     /locus_tag="ACHJ8C_004349"
     CDS             complement(1215..4328)
                     /gene="mdtF"
                     /locus_tag="ACHJ8C_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO5554105.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     ISATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIK
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKNNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVIGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPVEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4353..5462)
                     /gene="mdtE"
                     /locus_tag="ACHJ8C_004350"
     CDS             complement(4353..5462)
                     /gene="mdtE"
                     /locus_tag="ACHJ8C_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO5554106.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYGQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5570..5899
                     /locus_tag="ACHJ8C_004351"
     CDS             5570..5899
                     /locus_tag="ACHJ8C_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554107.1"
                     /translation="MRDLQTSGIVGLSASKMGCKHSDRHGEVNDKKNVAVLPTVSASI
                     RATKGSTGGYTYQGNKVVFVSPSIITGGIEISICIPRHASHQFQLLMSLLKIRCDTQG
                     DDVAHTS"
     gene            complement(5849..>6070)
                     /gene="gadE"
                     /locus_tag="ACHJ8C_004352"
     CDS             complement(5849..>6070)
                     /gene="gadE"
                     /locus_tag="ACHJ8C_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO5554108.1"
                     /translation="YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQT
                     LKIQKFNIMYKLKLRRMSDIVTLGITSYF"
BASE COUNT         1492 a         1505 c         1522 g         1551 t
ORIGIN      
        1 actttgtgga taaaatttgg tcctaccaaa tatggcagtt tttgcacaca gaaacagtct
       61 ggcgtcattt cattagtata ctgacattga aataatcgca gtaatgaaat ataagggata
      121 gtcatggctc atgtctgctc ggtgatcctc gttcgtcgtt cattcgatat tcatcatgaa
      181 cagcaaaaaa tatcgttgca taacgagagt atcctgctgc tggataaaaa tttggcagac
      241 gattttgcgt tttgttcact ggatacgcga cggctggata tcgaagagct gacagtttgc
      301 cattacttac aaaatattcg tcagttgcca cgcaatttag gattgcatag caaagaccgt
      361 ttgttaatta accagtcacc ccccatacag ctggtgacgg cgatttttga tagtttcaat
      421 gacccccggg tcaattcgcc gatactgagc aaaatgctct acctttcctg tttatcaatg
      481 ttttctcata agaaagaact gatcccctta cttttcaata gtatcagtac tgtttcagga
      541 aaagttgaac gccttattag ctttgatatc gctaaacgtt ggtatctacg cgatatcgca
      601 gaaagaatgt ataccagcga gagtctcatc aaaaaaaagt tgcaggatga aaatacctgt
      661 ttcagtaaaa tattactcgc ctccaggatg tcgatggcca gacgattact cgagttacgt
      721 caaatacctc tgcatactat tgcggaaaaa tgtggctata gcagtacgtc atactttata
      781 aatacattta gacaatatta tggtgtaacg ccacatcagt tttcgcaaca ttcgccgggt
      841 accttttcct gacatatttc gcatttgaat attggtcagg atctcacgtc tgcttcatgt
      901 gaaactcttt cctgatgatt tctgccgggc taccggctag ttctctttcg cagtttgcca
      961 ggcggtatag gtattttcag gaggaaaaga ctcggcatgt ttgggattat taagctaaca
     1021 attcatacga ttacgggaat gtgggtcagt attgtgctgt tcaaattgat gacgaatggc
     1081 tggagtggtt tctactttca gtgctgtgta ctaagcctgg tattcctgac ggttagctgg
     1141 ttgttgagtg gtgaatggtt agcaggaaag agtaaggctg aaccctcacg ttcaacctta
     1201 ctctcattta cacgttacgc ttttttaaag cgggcaaaga gatgttccac tacaacgaaa
     1261 aagaccggaa cgaagtaaat tgccagcact gttgcggcaa acatcccgcc cattacgccg
     1321 gtacctaccg cgttttgcgc gccagagccg gcaccatgac tgataaccag cggcagcacg
     1381 ccgagaataa aggccagaga agtcatcagg attgggcgta aacgcatacg cgctgcttcg
     1441 atgattgcct ctaccggcgt tttcccttct ttctgcatca tctcaacggc aaattcgaca
     1501 atcaggatgg cgttcttggc ggaaagcccg atggtggtca gcaaaccaac ctggaagtaa
     1561 acgtcattac ttaagccgcg cagatcggtg gccagtaatg cgccaacgac gcctaacgga
     1621 acaaccaaca tcaccgagaa cggaattgac cagctttcat aaagtgcggc gagggcgagg
     1681 aacaccacga ccagtgaaat ggcatatagc gcgggagcct ggtttgagga taacgcttcc
     1741 tgatacgaca gcccagtcca tgagtagccg acgcctgccg gaagttttgc gaccaggtct
     1801 gccataaatt tcatggcgtc accggtactt ttcccggcag ccgcttcacc taaaatctcc
     1861 attgacggga tgccgttgta gcgttccagt cgcggtgaac cataggtcca ttcggtagac
     1921 gaataggcag aaagcggtgc catcgtgcca gaggcgttgc gtacatacca ttggttgatg
     1981 ttatccggca acatacggaa cggcgtgcct gcctggacat acactttttt cacccgcccc
     2041 tggttgagga agtcgttcac gtagctgctg ccgaaggcgg tggaaattgt ctggttgata
     2101 tcagacagcg ccacacccat agcctcggct ttcgcagcgt tgacgttcac tttgaacatc
     2161 ggcgtatctt ccaggccgtt cgggcgtacc ccgatgacct gattcggtga ttgcgctgcc
     2221 agtgataaca gctcgtttcg cgcctgggtc agcttttcgt gccccagatt gccgttgtcc
     2281 agcagttcca tatcaaaacc tgacgcggta cccagttcag ccaccgcggg taagttgaac
     2341 gggaagacga cggctttatt gatactgctt agcgcaatca ttgcccgctg aataatcgcg
     2401 gtaaccgagt tttcctcgcc gacacgttca gaccacggct tgaggctgat aaacgccaga
     2461 ccgttgtttt gcccctgacc actgaagcca aagccaccaa cggtaaacac cgactgaaca
     2521 ttatttttct cttttgtcag ataataatcc gtcacctgtt gtagcacttt cgtggtgtta
     2581 accatggttg caccggaagg taactgcgcg gtggtcataa ataccccctg atcctcttct
     2641 ggtaagaaag aggtcggcgt acgcaggaac agcaccgcca tcccggcgca aatcagcagg
     2701 tagaccacca tgtagcgacc ggtacaacgc aacagcgagc gggtgctgtc ggtatagtgc
     2761 tgagttgatt tttcaaacag cgtgttgaag cgtgcgaaca gggcgttagg tttgtgaccg
     2821 ccctccggcg cggctttcag aatggtggcg cacagggcag gggtcaggct cattgctaca
     2881 aatactgaaa gcagcatgga ggagatcagc gtgatggaga actggcggta gatttccccg
     2941 gtcgcaccgc tcataaaggc catcggcata aacactgcgg aaagaacaac ggcaatcccg
     3001 accagcgcac gttggatctg ccccatcgat ttatgcgtcg cttccttcgg cggtagctta
     3061 tcttccgcaa tgacacgctc gacgttctcc accaccacga tggcgtcatc caccagtaac
     3121 cctatcgcca gcaccatccc gaacatagtt aacgtgttga tggtgaaacc gaccgccgac
     3181 aagatcgcaa acgtcccgag aataaccacc ggtacggcaa tcgtcgggat gattgtggcg
     3241 cggaaattct gcaaaaacag atacatgacc aggaagacga ggatgatagc ctcaaccagc
     3301 gttttgaaaa cttcctgaat agaaattttg ataaacggtg tggtgtcgta aggataaacc
     3361 gtcttcaggc ttgcagggaa ataagccgat aaacggttca gctcctcttt caccgctcgc
     3421 gaggtatcca gggcgtttgc tccggtagcc agtttgatgg cgatcccagc agcaggtttg
     3481 ccgttatagc gcgccacggt ggaataatct tccgccccaa gttcgacgcg ggcgacatca
     3541 cgcagcagta cttgcgaacc atcttgctga actttcaaca ggattttgcc aaattcttcc
     3601 ggcgtttgca gacgcgtctg cacaatgatc gaggcgttca gttgctggtc tgccgcctgt
     3661 ggcatgccac ccagttgacc accggaaatc tggttgtttt gcaccttaat ctgggaaata
     3721 acatcggaag gtaccaggtt atatttattg agtttttgcg ggtccagcca gatacgcatg
     3781 gcgtactcgg aaccaaagag ctgtacgcta ccaacgcccg cggtacggct tagcgggtct
     3841 ttgatattag acgctacata gtccgcgata tcgtactggt tgaggctgcc gttatcagaa
     3901 ataaatgccg ctaccatcag gatattactg ctcgacttat cgacgctaat cccctgctgc
     3961 tgcaccgctt caggtaatga aggcatagcg agttgcagtt tattttgcac ctgaacctgt
     4021 gcgatatcag gcgatgtccc cgtctcgaag gtcagagtga tggaggcatt acctgccgca
     4081 tcgctggttg aagacatgta catcaggcca tcaagcccat tcatattttg ctcaatcacc
     4141 tgagtgaccg agtcttctac cgtttgcgca tcggcacccg gataggttgc gctgatggta
     4201 atggtcggtg gcgcaatctg cggatactgc gcaaccggta agttcatgat cgccagacca
     4261 cctgcaagca tcataataat ggcaagtacc caggcaaaaa ccgggcgatc aataaaatag
     4321 ttagccatga aagtcccctt aagcctgcaa cgttattgtt tcgattcggt gctggcgttt
     4381 tcctggctgg aggaaattgc acgtgctttg atacccggac gaatgcgttg caaaccggaa
     4441 acgatcaccc gatcgccagc ctgcaagcca gaggtgacaa cccactggtc gccgatggct
     4501 ttgctggctt cgatttcgcg tagctgcacg acatcgtctt tatccagaat gagtgccgtt
     4561 gctttaccct gggcgttgtg ggtgacgcct tcctgcggca ccagtaatac attctggcgg
     4621 ctaccttcat ccactaatgc cgtgacatac atgccaggca gcaagtcacc atttgggttg
     4681 gggaaaatcg cccgtaacgt cacggagccc gtggtttcat ccaccgtcgg gtcggagaat
     4741 ttcagcgtgc cggtctggcc gtaacgttta ccattttcca gattgagctg tactggcgta
     4801 ctgccctgaa cctgtttgat ttgcccactg gcgacctctt ctttcatgcg caagaaatcc
     4861 tgcaccgact gcgtgagatc gacataaatc gggtccagac gttgtacggt aaccagcgaa
     4921 tctgcctgat tagcggtaac gagtgcgccg acggtcaccg acgatttccc gctgacgccc
     4981 gtaatcggcg aggtgacatt cgcgtattgc agattgatcg tcgcctgttc aacagccgct
     5041 ttggcgacgg tgacattggc ttctgcttca ttcaactggg tgcgcgcggt gtcgtaatcc
     5101 tgacggctaa catagttggt cttcagcaac gatgcctggc ggttaaaggt gatgcgggca
     5161 ttgctggcgg tagagagcgc tttcgccagc gagcctttgg cggagttcag ctcagcctgt
     5221 aaaggcgcag gatcaatctg atacagcgaa tcgccctggt tcactttatc gccttcgata
     5281 aagttgcgtt taatgataat tccgcccacc tggggacgta tctcggcaac ttcataagga
     5341 acggttctac cgggcaattc gctcaacaca ttgaccgaac cgggggagag tgtgacgaca
     5401 ccgacctcag gcgtcatggc ggcggcgttt tccgccgatt tgtcatcgca ggcggtgagt
     5461 atcgcgccgc agaataacaa cggtattaac agttttcttc ttctgttcat tttagtccct
     5521 gaaaattctt gagaaataac ggttctttaa attgcgcgcg aggagagata tgagggattt
     5581 acagaccagc ggcatagtgg ggttgtcagc cagtaagatg ggatgcaagc acagtgatcg
     5641 acatggtgag gtcaacgaca aaaaaaatgt tgccgttctg ccaacagttt ctgccagcat
     5701 tcgggcaacg aagggttcta ctggtggata cacataccag gggaataaag tcgttttcgt
     5761 ctctccttca ataatcaccg ggggtattga aatcagcatc tgcatccctc gtcatgccag
     5821 ccatcaattt cagttgctta tgtccttact aaaaataaga tgtgataccc agggtgacga
     5881 tgtcgctcat acgtcttaat ttcagcttgt acataatgtt gaatttctga atctttaatg
     5941 tttgttgcga aatgttgagc gttgacgtga tattgctttc atttttgttt ttcagaatca
     6001 gttgcataat tttgaactga ttatgtgaaa aacatagttg tgaattctta tggggcaagt
     6061 gtttaccata
//
LOCUS       DAZFJL010000192         6057 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFJL010000192 DAZFJL010000000
VERSION     DAZFJL010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.194"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..374)
                     /locus_tag="ACHJ8C_004353"
     CDS             complement(<1..374)
                     /locus_tag="ACHJ8C_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFO5554109.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNAT"
     gene            complement(478..1215)
                     /gene="bamD"
                     /locus_tag="ACHJ8C_004354"
     CDS             complement(478..1215)
                     /gene="bamD"
                     /locus_tag="ACHJ8C_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO5554110.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQLNAQAEKVAKIIAANSSNT"
     gene            1350..2330
                     /gene="rluD"
                     /locus_tag="ACHJ8C_004355"
     CDS             1350..2330
                     /gene="rluD"
                     /locus_tag="ACHJ8C_004355"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO5554111.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPEDMVELIEVMRADFEEHKDEVDWL"
     gene            2327..3058
                     /gene="yfiH"
                     /locus_tag="ACHJ8C_004356"
     CDS             2327..3058
                     /gene="yfiH"
                     /locus_tag="ACHJ8C_004356"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO5554112.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEIAAAHAGWRGLCSGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASTAFIQHGDKYLADIYQLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            3188..5761
                     /gene="clpB"
                     /locus_tag="ACHJ8C_004357"
     CDS             3188..5761
                     /gene="clpB"
                     /locus_tag="ACHJ8C_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO5554113.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMIRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1511 a         1457 c         1656 g         1433 t
ORIGIN      
        1 gtggcgttat gccagagctg cgctgttttc gtgaacacgc ggcggacagc ctcacttccc
       61 aggcgctggc gggcctgaat aacggcactg ggggcaacga aggggcgatt gcccggcagc
      121 atgatgtcca ggcgattcac aatctggtga agaggttctt tacgctcaag cgccatgcca
      181 acaatacacc agaccatcat ttcgagggga agacggcgct tgcgtagcgt tacagtacct
      241 gattcggcaa ggcaacgaga gatgagttcg gggtcgaggt aatcccccag agaagtcagt
      301 gggttacgca gagaatcgta acgggatacc agatcaagag cctgtccaat gtgcataaaa
      361 aaatccggaa acaagtgagc gtttccggat tcttacacag ccactggatc ggtcaactga
      421 tccttaactg atcggcatta cagctcaagg gctgccgttt tgtgtttcag gtttctgtta
      481 tgtattgctg ctgtttgcgg cgatgatttt cgctactttt tcagcttgcg cattcaactg
      541 catctgacgg tatgcatttt ccatcagcgg cagcgcatca cgcgtagcct gggtatccgg
      601 gtagtcgcgc aacatgcctt ctacgcggtt aacgacggca acccatgcac cacgttctgt
      661 atagtactcg gcaacggagt attcatattt cgccagacga tctttcagga ataccagacg
      721 tttggtggca tcggtggtgt actgactgtt cggatagccg cgcaccagtt tggaaaagtc
      781 actaaacgca gctcgtgcat gttgaggatc gcgatcgcta cggtcaacgc caaagaaccc
      841 ttgcagcgca ctgtcatcca gcgccatatt ggtcaggcca cgcatgtaca tgacataatc
      901 gatattcgga tgggtcgggt taaggcgaat aaaacgatcg atggcagcct gtgctaacgg
      961 caaatcggcg tttttatagt aggcgtagat gagatccagc tgcacctgct gcgaatacgg
     1021 accaaacgga tagcgattat ctaacgcttc cagttgcgtt attgcctgtc tccagttacc
     1081 gtcctgcagc ttttgttgtg cagtcgcgta aatttcattt ggcggattat caggtacttc
     1141 ttcctttgac cccgagcaac ccgccaaaaa caggcttagt gtggcggctg ccaccagata
     1201 tttcatgcgc gtcatgacgt tttgactttc ctcaaaatgt aatacgggag attctctgtt
     1261 cctgctcccg gttaagacca gctacaatag cacactatat taaacggcaa agccgtaaaa
     1321 ccccaacgat aaacgaagaa gcagtatata tggcacaacg agtacagctc actgcaacgg
     1381 tgtccgaaaa ccaactcggt caacgcttag atcaggcttt ggccgaaatg ttcccggatt
     1441 attcacgttc gcgaataaaa gaatggatcc tcgaccagcg cgtgctggtt aacggcaaag
     1501 tttgtgataa gccgaaagaa aaagtattgg gtggcgagca ggtggccatc aacgctgaga
     1561 ttgaagaaga agcgcgtttt gaaccgcagg atatcccgct ggatatcgtc tatgaagatg
     1621 aagatatcat cgtcattaat aaaccacgcg acctggtggt acatcctggc gcgggtaacc
     1681 cggatggtac ggtactgaat gcgttgcttc attattatcc gcccattgcc gatgtaccgc
     1741 gtgcgggcat cgtccatcgt ctggataaag acaccaccgg cctgatggtt gtggcaaaaa
     1801 ccgttccggc tcagacgcgt ttagtcgaat ctttgcaacg gcgtgaaatt actcgtgagt
     1861 atgaagcggt ggcaattggt catatgaccg ctggcggtac agtagatgaa ccaatcagcc
     1921 gccacccgac caaacgtact cacatggcgg tgcatccgat gggtaaaccg gcggtcactc
     1981 actatcgcat catggaacac ttccgtgtgc acacgcgtct gcggttgcgt ctggaaaccg
     2041 gacgtacgca ccagatccgc gtgcatatgg cccatatcac tcatccgctg gtgggcgatc
     2101 cggtttatgg tggccgtccg cgtccgccaa aaggtgcttc ggaagcgttt atctccacgc
     2161 tgcgtaagtt tgaccgccag gcgcttcatg caaccatgct gcgtctttat cacccgatct
     2221 ccggtatcga aatggaatgg catgcgccta ttccagaaga tatggtggag ctgattgagg
     2281 tgatgcgcgc cgatttcgaa gagcataagg atgaagtgga ctggttatga gtaagctgat
     2341 tgtcccgcag tggccgctgc caaaaggtgt tgcggcctgt agctccactc gtatcggcgg
     2401 cgtgagcttg cccccgtatg attcgctcaa cctcggtgct cattgtggcg ataacccgga
     2461 tcacgttgaa gagaatcgca agcgactttt tgctgcgggc aatttgcctt caaaaccggt
     2521 ctggcttgag caggtacacg gcaaagatgt gcttaagctt actggcgaac cttatgcctc
     2581 aaaacgagca gatgcctctt atagcaacac gccgggcacg gtttgcgcag tgatgactgc
     2641 cgactgcctc cccgtgctgt tttgcaatcg agcgggaacg gaaatcgccg ccgctcatgc
     2701 tggctggcgt ggactgtgct caggcgtgct ggaagagacg gtttcctgtt ttgctgataa
     2761 tccggaaaat attctcgcct ggttagggcc ggcaattggt ccacgcgcgt ttgaagtggg
     2821 ggcggaggtt cgcgaggcgt ttatggcagt agacgccaaa gcgagtacag ctttcattca
     2881 gcatggtgat aagtatctgg cagatattta tcagcttgcc cggcagcgtc tggcgagcgt
     2941 tggtgttgaa caaattttcg gcggcgaccg ttgtacatat acggaaaatg agactttctt
     3001 ctcttatcgt cgcgacaaga ccaccggtcg tatggcaagt ttcatttggc tgatataacc
     3061 taaagaatca agacgatccg gtacgcgtga ttttcttttc acattaatct ggtcaataac
     3121 cttgaataat tgagggatga cctcatttaa tctccagtag caactttgat ccgttatggg
     3181 aggagttatg cgtctggatc gtcttactaa taaattccag cttgctcttg ccgatgccca
     3241 atcacttgca ctcgggcacg acaaccaatt catcgaacca cttcatttaa tgagcgccct
     3301 gctgaatcag gaagggggtt cggttagtcc tttattaaca tccgctggca ttaatgctgg
     3361 ccagttgcgc acagatatca atcaggcatt aaatcgttta ccgcaggttg aaggtactgg
     3421 tggtgatgtc cagccatcac aggatctggt gcgcgttctt aatctttgcg acaagctggc
     3481 gcaaaaacgt ggtgataact ttatctcgtc agaactgttc gttctggcgg cacttgagtc
     3541 tcgcggcacg ctggccgaca tcctgaaagc agcaggggcg accaccgcca acattactca
     3601 agcgattgaa caaatgcgtg gaggtgaaag cgtgaacgat caaggtgctg aagaccaacg
     3661 tcaggctttg aaaaaatata ccatcgacct tacagaacga gccgaacagg gcaaactcga
     3721 tccggtgatt ggtcgtgatg aagaaattcg ccgtaccatt caggtgctgc aacgtcgtac
     3781 taaaaataac ccggtactga ttggtgaacc aggcgtcggt aaaactgcca tcgttgaagg
     3841 tctggcgcag cgtatcatca acggcgaagt gccggaaggg ttgaaaggcc gccgcgtact
     3901 ggcgctggat atgggcgcgc tggtggctgg ggcgaaatat cgcggtgagt ttgaagaacg
     3961 tttaaaaggc gtacttaacg atcttgctaa acaggaaggc aacgtcatcc tgtttatcga
     4021 cgaattacat accatggtcg gcgcgggtaa agccgatggc gcaatggacg ccggaaacat
     4081 gctgaaaccg gcgctggcgc gtggtgaatt gcactgcgta ggtgccacga cgcttgacga
     4141 atatcgccag tacatcgaaa aagatgctgc gctggaacgt cgtttccaga aagtgtttgt
     4201 tgccgagcct tctgttgaag ataccattgc gattctgcgt ggcctgaaag aacgttacga
     4261 attgcaccac catgtgcaaa ttactgaccc ggcaattgtt gcagcggcga cgttgtctca
     4321 tcgctacatt gctgaccgtc agctgccgga taaagccatc gacctgatcg atgaagcagc
     4381 atccagcatt cgtatgcaga ttgactcaaa accagaagaa ctcgaccgac tcgatcgccg
     4441 tatcatccag cttaaactgg aacaacaggc gttaatgaaa gagtctgatg aagccagtaa
     4501 aaaacgcctg gatatgctca acgaagaact gagcgacaaa gaacgtcagt actccgagtt
     4561 agaagaagag tggaaagcag agaaagcatc gctttctggt acgcagacca ttaaagcgga
     4621 actggaacaa gcgaaaatcg ctattgaaca ggctcgccgt gtgggggacc tggcgcggat
     4681 gtctgaactg caatacggca aaatcccgga actggaaaag caactggaag ccgcaacgca
     4741 gctcgaaggc aaaactatgc gtctgttgcg taataaagtg accgatgccg aaattgctga
     4801 agtgctggcg cgttggacgg ggattccggt ttctcgcatg atggaaagcg agcgcgaaaa
     4861 actgctgcgt atggagcaag aactgcacca tcgcgttatt ggtcagaacg aagcggttga
     4921 tgcggtatct aacgctattc gtcgtagccg tgcggggctg gcggatccaa atcgcccgat
     4981 tggttcattc ctgttcctcg gcccaaccgg tgtggggaaa actgagcttt gtaaggcgct
     5041 ggcgaacttt atgtttgata gcgacgaggc gatgatccgt atcgatatgt ccgagtttat
     5101 ggagaaacac tcggtgtctc gtctggttgg tgcgcctccg ggatatgtcg gttatgaaga
     5161 aggtggctac ctgaccgaag ctgtgcgtcg tcgtccgtat tccgtcatcc tgctggatga
     5221 agtggaaaaa gcgcatccgg atgtcttcaa cattctgttg caggtactgg atgatgggcg
     5281 tctgactgac gggcagggga gaacggtcga cttccgtaat acggttgtca ttatgacctc
     5341 taacctcggt tccgatttga ttcaggaacg cttcggtgaa ctggattatg cgcacatgaa
     5401 agagctggtg ctcggtgtgg taagccataa cttccgtccg gaattcatta accgtatcga
     5461 tgaagtggtg gtcttccatc cgctgggtga acagcacatt gcgtcgattg cgcagattca
     5521 gttgaaacgt ctgtacaaac gtctggaaga acgtggttat gaaatccaca tttctgacga
     5581 ggcgctgaaa ctgctgagcg agaacggtta cgatccggtc tatggtgcac gtccgctgaa
     5641 acgtgctatc cagcagcaga tcgaaaaccc gctggcacag caaatactgt ctggtgaatt
     5701 ggttccgggt aaagttattc gcctggaagt taatgaagac cggattgtcg ccgtccagta
     5761 atgataaaac gagcccttcg gggctcgttt ttgtctataa attagacgga aaaggctatg
     5821 tttaagatgt tttgcctgaa aaatgagcga acgataaagt ttttatattt tttgcttgtc
     5881 aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc aaacacgccg
     5941 ccgggttagc ggggttctcc tgagaattcc ggcagagaaa gcaaaaacaa atgcttgact
     6001 ctgtagcggg aaagcgtatt atgcacaccc cgcgccgctg agaaaaaagc gaagcgg
//
LOCUS       DAZFJL010000193         5951 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZFJL010000193 DAZFJL010000000
VERSION     DAZFJL010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.195"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(119..1606)
                     /gene="yeeO"
                     /locus_tag="ACHJ8C_004358"
     CDS             complement(119..1606)
                     /gene="yeeO"
                     /locus_tag="ACHJ8C_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO5554114.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            1767..1842
                     /locus_tag="ACHJ8C_004359"
     tRNA            1767..1842
                     /locus_tag="ACHJ8C_004359"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1800..1802,aa:Asn,seq:gtt)
     gene            complement(1880..2830)
                     /gene="cbl"
                     /locus_tag="ACHJ8C_004360"
     CDS             complement(1880..2830)
                     /gene="cbl"
                     /locus_tag="ACHJ8C_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO5554115.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2932..3849)
                     /gene="nac"
                     /locus_tag="ACHJ8C_004361"
     CDS             complement(2932..3849)
                     /gene="nac"
                     /locus_tag="ACHJ8C_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO5554116.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVMSSPVMEKRQWQLVS"
     gene            4176..4251
                     /locus_tag="ACHJ8C_004362"
     tRNA            4176..4251
                     /locus_tag="ACHJ8C_004362"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4209..4211,aa:Asn,seq:gtt)
     gene            complement(4310..4870)
                     /gene="hxlB"
                     /locus_tag="ACHJ8C_004363"
     CDS             complement(4310..4870)
                     /gene="hxlB"
                     /locus_tag="ACHJ8C_004363"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFO5554117.1"
                     /translation="MSVQENTINILHELENNALKIDDSQAAQFISQIRNARHIFLQGA
                     GRSGIAIRAFANRLLHLGFSVSVVGEISSPHTQTGDLLIIGSGSGETTSLKSLAQKAV
                     DSGVNVALVTMKADSTIGKLAQSVLVLPGSVKDDNNRVAGTFAQPMGSAFEQLCFITY
                     DAIVLALMSELGESSATMFTRHAELE"
     gene            complement(4886..5638)
                     /locus_tag="ACHJ8C_004364"
     CDS             complement(4886..5638)
                     /locus_tag="ACHJ8C_004364"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFO5554118.1"
                     /translation="MSNFAGKVVLVTGGAHGIGKGIAKQFADRGAHTVIVDYNEENGK
                     ATAAELTFGDVKSLFVKTDVSDPQALVDVREKVFATFGRIDVLILNAGVAFANKVNNI
                     SFEEWNKTIAINLSGLFNTVKAFYDDFLTNKGSIVYISSGSALSGTGGGVSYPASKAG
                     GEGLIRGLAKELGPKGVNVNAIAPRLIDTGEMMRVNYPTQADLDAVLEKIPVRRLGTI
                     DDVANLAIFLADKDNSYIQGQTILMDGGRTIA"
     gene            complement(5734..>5951)
                     /locus_tag="ACHJ8C_004365"
     CDS             complement(5734..>5951)
                     /locus_tag="ACHJ8C_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO5554119.1"
                     /translation="GQTFIHKSLEKIRNTKKMISGRDIQIEVDGGVNLDNVKQVKEAG
                     ADLIVVGGALFNKQPHESYQALKIAVQ"
BASE COUNT         1689 a         1533 c         1207 g         1522 t
ORIGIN      
        1 cgactgaact acagaggaat cgtgagaacg aggcgtatat tagcgatgcc catccacaat
       61 gtcaaagcct gttttttaaa tttgaaatcg tttgctgaaa taatctgcat tttgtcgttt
      121 attccgacac aactggcttt ttttcacact tttgcggctc ggatcgaggg tatttccaca
      181 gccaacgtcc agtaaccatt cgccagtaaa acagcacggc ccgcacagcc cagtcggcaa
      241 acattcccat ccagacacca accacacccc agccaagcat gattcccagc acataaccga
      301 ctacaacccg gcaaccccac atgctcaaca tcgaaaccca catggcgtaa cgggcatcac
      361 gagcaccctt aaatccagcg ggtagcaccc atgaggcgga ccaaataggc ataaataaag
      421 catttagcca aatcagaatc acaacgacgt gtttaacctg tggatcctgg gtgtaaaacg
      481 atgccataac cccggcaaag ggagccgtta gccaggcgat ggccgttaat ccaagagtgg
      541 acagccagaa cacatgccgc aactgaatct ctgcttgcgc tatctgccct acccccaacc
      601 ttcggcctgt aatgatcgta gaagcagaac cgagcgcact tccgggtaag ttgataagag
      661 ccgcaattga gaacgcgata aaatttccgg caataacgct ggtccccatc ccggcaacga
      721 acatttgggt taataaccga ccactggtaa ataacactga ttcgacactc gctggaatac
      781 caatccccat gacttcccag ataatgctaa aattcagcgg tttaaaatag ctctttaacg
      841 aaatccttag cgcaggatta aaaccaatcg ccagcaccca caaaattgca actgcgccaa
      901 tataacgaga aatggttaaa cccaacccag ccccgacaaa tcccagtccc ggccaggaga
      961 aaaggccgta aatcaatatg ccgctaataa taatattaag aatattcagg ctaccgttaa
     1021 tcagtagcgg tattttcgta ttccctgcac cacgaagtgc cccgctacca ataagagtga
     1081 tggcagctgc tggataactg agtacggtca gctccagata agtcaacgcc agtgctttaa
     1141 cttctgtcgt ggcatcaccc gcgacgaaat caataatttg ttcgccaaaa tgatgaatta
     1201 gcgttgccaa cagtacggca aacaacgtca tgatcaccaa tgactgccgc gtcgccaccc
     1261 tcgctcgtcg tcgatctcgc ttaccgagac taaatgctac aacgacagta gtaccaagat
     1321 cgatagctgc aaaaaaagcc ataatgacca tattgaagct gtccgccaac cccacgccgg
     1381 ccatcgcatc ttttcccagc cagctgacca gaaaagtgct caggaccccc atcaacagga
     1441 cacaggcgtt ctccatgaag ataggaacag caagcggggt tatctcgcgc cagaacaaca
     1501 ctttgtagct cttgcgttta gagtgccagc gagtgccgtg aacaacctgg cgtaaagcag
     1561 aggagatatt caaagccgac cttaattgca gaaagtgaaa ccacatttca aatgatgagg
     1621 gagaatcagc aaagctgcaa agattttcgc caacaaattg tctgcaaatg caacaaactg
     1681 ttgatagaaa cggcaaacag ttgggaaatt taaaaatcgg gtttgacaaa atatttttcg
     1741 ccgttaagat gtgcctcaac agcgattcct ctgtagttca gtcggtagaa cggcggactg
     1801 ttaatccgta tgtcactggt tcgagtccag tcagaggagc caaatttctg aaaagcccgc
     1861 ttttatagcg ggctttttgc tatatctgat aatcaatttc ctcttcactg ttttccatca
     1921 cctggcgctt gatatcctca actgacagtc ctgcattaca gagttccaga aagcgccaga
     1981 catagttacg ctgaagttgc cctcgcttca gtcccaacca gacagtatta gcatcgaaaa
     2041 gatgccgcgt atccaggcgg attaaattct tttcctcttg ttcgccgctg gattgttcgg
     2101 caactaatcc gatcccaagc ccaagagcaa cataggtttt aatgacatca gaatcctgtg
     2161 cacttaatac aatatctgcc ggcaaacctt tgcgggcaaa tgcgtcatca atgcgtgagc
     2221 gccccgtaat tccctgtcgg taagtaatta acggccactt cgctattgat tccagcgtca
     2281 atggtgtaat ttgcgttaag ggatgatcga gtggaacaag caaactatgg tgccagcgaa
     2341 accacgggaa ggcgacgagc tgcgggtcat tactcaaacg ctcgctggcg ataccaatat
     2401 cagcttcgcc attttgcaac aacgtcgcaa tttcctgtgg cgtaccctgg attagctcca
     2461 gccgaacctc cgggaaaagt tcgcgaaaag ctttaatgac ctctggcaag ctataacgtg
     2521 cctgagtatg cgtcgttgca atagtgagaa caccagacgt atcgttggta aacaagtctg
     2581 caagccgacg aacattactg gcttcattca gaatacgttc tgcaatgacc agtaatgctt
     2641 tgcccggttc agtcatgccc agcagtcgct tacctcgtcg aacaaatatt tcgatgccaa
     2701 gttcatcctc cagttcccga atatgacggc taacgcctga ctgtgaggta aaaagcatat
     2761 tcgcaacctc tgtcaggttg taatcctgac gtgcagcctc gcggattatc tttagttgtt
     2821 ggaaattcac ggtaaactcc gggcagttca gatatcccgt tattgttaaa gtctaatgcc
     2881 cggcataaca aataataaaa acccgcatct tattccatcc cgatataacg cttagctcac
     2941 caattgccac tgcctttttt ccatcactgg agaactcatc actgacatta acaactcttt
     3001 cactgcctgt gcctgtggtg acaagtttgc tctggcgggt aaatttaagg acaaagagag
     3061 actcattgaa ggagtggtaa tgcgtgacat ccacccattt actgcgccac ataacgaacg
     3121 cgcagccgat tcaggtaata ctgcaacgcc catgccgctg gcaatcgctg cggtaagcgt
     3181 ggcaatagac tcaatttcac caatgacttt tgccgtgagc cgccgtaggg aaaaagcctc
     3241 atcaacacga agtctaacag cgctgtaatc actggggaga aaaaggttca tttgcgcaat
     3301 agcattaaca tcaacgtttt gccccgggca atcttgagtt cctaccagga aaagatcttc
     3361 tttcagcaaa gcctgactgg ataccccagc cacaggggaa tgctcataaa tcaccgccat
     3421 atcgagttgg tgatttatca gtttttcgtt aagcactgca ccactatttt catgaagata
     3481 gataacgacc tccgggaatt cagcgcgaac cgcctgtaat aagggcatgg tgatggatga
     3541 cgcagcggtt cctggcgcaa agccaatcga gacttgcccc gataatgact gaccaacgtt
     3601 atgcaccgcc agttgggcct gctcacactg acgtaaaatg gcccgtgcat gggtatagag
     3661 aatttttccg gcgtctgttg gcgtaacgcc ccgttttgtg cgaatcaaaa gctgttgatt
     3721 taactcaccc tccagtgtgg caacctgctg gctgagcgct ggttgtgcga tatgcaatac
     3781 ttcagcagcc tgggtcaggc taccaatatc tacaattttt acgaagtatt tcaggcgtct
     3841 gaagttcatg ttgcctccgg tttttaagaa tcggcccaag tgccgccatt acttacaacc
     3901 agaatgcaag atgcttgcca gttctatttt ggcgttgatg cgcaagctaa ccaactgtca
     3961 aataagagat catgatagat tcgtcattgg ccctttttat cagcagtcgc attcacctgc
     4021 cctataaaag gagggtatgc accacgatgg ttcattaccc aataagattg aaagctcacc
     4081 actttgttga aattggcagc aaacaaacaa aaaaatgcat ttcacccttt gacatcacca
     4141 tgcactgcca ttaatatgcg ccccgttcac acgattcctc tgtagttcag tcggtagaac
     4201 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aaattcaaaa
     4261 aagcctgctt tctagcaggc tttttgcttt ctaattacca actctttggt cattccagtt
     4321 cagcgtgtcg ggtaaacatt gtggcactgc tttcacccag ttccgacatc aacgccagaa
     4381 caattgcatc ataagtaata aagcagagtt gctcaaaagc tgagcccatt ggttgcgcga
     4441 aagtgcctgc aacacgattg ttatcgtctt tcacacttcc tggtagtacc aacacagatt
     4501 gagctaattt accgatcgtc gaatcagctt tcatagtgac cagagcgacg tttactccgc
     4561 tatcaactgc tttttgtgcc agacttttca aactggtagt ttcaccactg cctgacccaa
     4621 taattaatag atctccagtt tgggtatgcg gtgatgatat ttctcccaca acgcttactg
     4681 aaaaacctaa gtgcagcaag cgattagcaa aagcgcgaat agcaatgcca ctacgcccag
     4741 ctccctgcaa aaaaatatgc cgggcatttc tgatttgcga aataaactgt gcagcctgag
     4801 aatcgtcaat cttcagcgca ttattctcta attcatgcag aatattaata gtattttcct
     4861 gaacactcat tttttctctt cctttttaag caatagtccg gccaccatcc attaaaatgg
     4921 tttgtccctg aatataagag ttatctttat ccgccaaaaa tattgccagg ttagccacat
     4981 catcgatagt ccccagacga cggacgggta ttttttccag gacagcgtct aaatcggctt
     5041 gtgttggata attgacccgc atcatctctc cggtgtctat taagcgagga gcgatcgcat
     5101 taacattgac gccttttggt cctaactcct ttgccagacc gcgaatcaac ccttcacctc
     5161 ccgctttgct ggcaggataa ctcacaccgc ccccagtccc cgaaagtgct gatcccgatg
     5221 agatataaac aatagatccc ttatttgtca ggaaatcatc ataaaacgcc ttaacggtat
     5281 taaataatcc cgataagtta attgcgatag tcttattcca ttcctcaaaa gaaatattgt
     5341 taactttatt ggcaaatgcc acaccggcat ttaatatcaa cacatcaata cgaccaaagg
     5401 tagcaaagac tttttcccgt acatccacta atgcctgtgg atccgagaca tctgttttaa
     5461 caaacagcga tttaacatcg ccaaaagtta actctgctgc agtggccttg ccgttctctt
     5521 cgttatagtc aacaataacc gtatgtgcac cacgatcagc aaattgttta gctatccctt
     5581 tgccaatacc gtgagcacct ccggtaacta aaaccacttt acctgcaaaa ttactcatta
     5641 cttttctcca ttaaagactt tttagttgag gttttcctcc cgatatgaaa tcgaaaacct
     5701 cacttagctg gatgtaaaat ttttagattg tgatcactga acagcgattt ttaatgcctg
     5761 ataactttca tgaggctgtt tgttaaataa tgcaccgcca acaacaataa ggtcagcccc
     5821 cgcttcttta acttgtttca cattatcaag attaacgcca ccatccactt caatttgaat
     5881 atctctgcca gatatcattt ttttggtatt acggattttc tccagacttt tatgaataaa
     5941 tgtctggcct g
//
LOCUS       DAZFJL010000194         5772 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFJL010000194 DAZFJL010000000
VERSION     DAZFJL010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.196"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..752)
                     /gene="tolA"
                     /locus_tag="ACHJ8C_004366"
     CDS             complement(<1..752)
                     /gene="tolA"
                     /locus_tag="ACHJ8C_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO5554120.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAVEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAA"
     gene            complement(817..1245)
                     /gene="tolR"
                     /locus_tag="ACHJ8C_004367"
     CDS             complement(817..1245)
                     /gene="tolR"
                     /locus_tag="ACHJ8C_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO5554121.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(1249..1941)
                     /gene="tolQ"
                     /locus_tag="ACHJ8C_004368"
     CDS             complement(1249..1941)
                     /gene="tolQ"
                     /locus_tag="ACHJ8C_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO5554122.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLSGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(1938..2342)
                     /gene="ybgC"
                     /locus_tag="ACHJ8C_004369"
     CDS             complement(1938..2342)
                     /gene="ybgC"
                     /locus_tag="ACHJ8C_004369"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO5554123.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(2492..2785)
                     /gene="ybgE"
                     /locus_tag="ACHJ8C_004370"
     CDS             complement(2492..2785)
                     /gene="ybgE"
                     /locus_tag="ACHJ8C_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO5554124.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(2785..2898)
                     /gene="cydX"
                     /locus_tag="ACHJ8C_004371"
     CDS             complement(2785..2898)
                     /gene="cydX"
                     /locus_tag="ACHJ8C_004371"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO5554125.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(2913..4052)
                     /gene="cydB"
                     /locus_tag="ACHJ8C_004372"
     CDS             complement(2913..4052)
                     /gene="cydB"
                     /locus_tag="ACHJ8C_004372"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO5554126.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(4068..5636)
                     /gene="cydA"
                     /locus_tag="ACHJ8C_004373"
     CDS             complement(4068..5636)
                     /gene="cydA"
                     /locus_tag="ACHJ8C_004373"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO5554127.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         1460 a         1609 c         1376 g         1327 t
ORIGIN      
        1 tctgctgctt ttttgtcggc tgccgctttc tctgctgccg ctttcttatc agctgcagcc
       61 ttttcagcag ccgctttctt ctcagcttct gccttggctt tttcagcagc ttcggttgcc
      121 gctttctttc ttgcttcagc tgcagctgct tctgccgctt cagctttctt cttcagcgcc
      181 gcagcagcag cctcggcttt tttctgcgct tcgactgcgg ctttggcggc ttctgcttct
      241 gcttttttct tcgcgtctgc cgccgctttc ttcgctgctt cttccgcagc tttagcatcc
      301 gcttcggcct tcgctttagc atctgccgcc gctttcgctg ccgcctcttc cgcttgcttc
      361 tgctttaact cggcctgttt tgcggcttct tcagcctgct ttttctgctc ctgagccgct
      421 aaccgctctt tctcaagttg cttcaggcgt tcctgttcag ccgcttgttt ctcacgcagt
      481 tcttcagcag cctgctgctc cttcatcttg cgctgctcat cagaacgctt cgcgcttgat
      541 tcctggcttt gcatacgttt gtactgctca actaccgcac ctgaatcaac catgacagcg
      601 tcgatggacg aaccaccgcc gcctccggct gaagcttcta tattctcatc gaacgaactc
      661 cagatcagcg ccgcaaataa gatgacatgc agcactgctg aaataattat cgcccgcttg
      721 agcttgtcgt tttgttcggt tgcctttgac actctcggtt tccaaaaact gttcgcctgt
      781 tacccgctct ctttcaagca agggaaacgc agatgtttag ataggctgcg tcattaaacc
      841 aaccgatttc acacccgcac tatgtaacaa gttcagtgct ttaattattt catcgtaagg
      901 cacatctttt gcgccaccga tcagaaagac cgttttcggg ttggccttga aacggctgga
      961 cacttccgcc accacctgct ctggtggtaa acgctccaga cgatctttct caaccaccac
     1021 ggtgtactga ccaataccag acacttcaac aatcactggc ggattatcgt tactgctcac
     1081 cgcctgtgat tcagtagcgt ctggcagatc gacctccacg ctctgggtga tgatgggcgc
     1141 tgtcgccata aagatcagta acagcaccag cagtacgtcc agcaacggta caatgttgat
     1201 ttcggacttg agatcgcgac gacctcgtcc acgcgctctg gccatggctt accccttgtt
     1261 gctctcgcta acggtaaacg cctggcggtg cagaatcgcg gtaaactctt ccataaagtt
     1321 gtcgtaattc agttccagtt tgtttacgcg ctggtttagg cggttataag ccattaccgc
     1381 cggaattgcg gcgaacaaac caattgccgt cgcaatcaac gcttctgcga taccgggcgc
     1441 aaccatttgc agtgttgctt gttttaccgc cccgagggcg ataaaggcgt gcatgatccc
     1501 ccagaccgta ccaaacagac caatatacgg gctgatggag ccaaccgtac cgaggaaagg
     1561 aatgtgcgtt tccagatttt caagctcacg attcatggaa atacgcatcg cacgcgacgc
     1621 cccttccact acggcttccg gcgcatggct attggcacga tgcaggcgca caaactcttt
     1681 gaagccgctg taaaagattt gttccgaacc gctcagatta tcccgtttcc cctggctctc
     1741 ttgatagagg cgagagagtt cgattccaga ccagaattta tcttcaaacg cttcggcttc
     1801 gcgcgccgct gcgttaagaa tacgggtccg ctggataata atggcccagg atgcgattga
     1861 aaaaccaatc aaaatcaaca tgataagttt aaccagaagg ctagccttca ggaacaaatc
     1921 aaggatattc atgtcagtca ctgcttaaac tccgcgacaa tagacttggg aagcgcacga
     1981 ggcttcattt tgagtgggtc aacgcaaaca accagaacct ctgcttcatt caacaaagta
     2041 ttctcggcgt tgacaatacg ttgcgtgaaa accaaagagg tgccacgcat tgatgttatt
     2101 tcagtctgta tttcgagcat atcgtcgagc cgcgcaggtg cgtaatattc caccgtcatt
     2161 ttacgtacca caaaggcaac gcgttcagcc atcagcgcct gttgactgaa gtgatgatga
     2221 cgcagcatct ctgtgcgtgc tctttcataa aaagcgacgt aactggcgtg gtacaccaca
     2281 ccaccggcat cggtatcttc atagtagaca cgaaccggcc atcgaaacag cgttgtattc
     2341 actttacatc ccggtaatgc aacaaaagtt agagctttta aacgctgcta ctatacgcgc
     2401 gcgattgagg tttgggaatg agggcaagtt aagggagcgt aaaaatttat gggcctgact
     2461 cggcccataa atttttaaag agaattctga tttaaaagaa gaagaaaatc agcccgacaa
     2521 tgagaacaat atcggcaagc aatgggcaaa aaatcccttg ccagagaacc ttctgcggac
     2581 gaaagcccac gccgtgaatc acaccagcac agacggccca cataagcagt aaaccatgcc
     2641 agatttccag ttcactggtc ttcgcggcga aacgtgatgg gtcccaaaac atacatcctg
     2701 ctaacagaag tgccatcacg aaggaaagcg cccgcagggg gcgcttgtcc attaccgcat
     2761 acaaggttgc gataatctta ctcatcagat gtcttcttga ccggctttgc ctgattcgac
     2821 gtgctcaagc gccagcgcgg tgattacccc aaacgaacag gcaagaagcg ttcccagaat
     2881 ccatgcgaaa taccacattt ttagctcctt acttagtaca gagagtgggt gttacgttca
     2941 atatcttctt tggtgatacg accgaacatt ttccagtaac accaggcggt gtagagcaga
     3001 atgatcggta ccagaaccac cgcaacccag gtcatgacgt taagcgtcag ctggctggaa
     3061 gttgcatccc acattgtcag acttgcgttc atcatggtgc tggacggcat cacaaacggg
     3121 aacattgcga tacctgcagt caggatgatg caggccagcg tcagggagga gaacacaaac
     3181 gcccacgcgg ctttatccat acgtgcagtc aggatggtca gcagcggcag aaccacaccc
     3241 agtgccggaa tagcccacag aattggcgtg ttgttgaagt taaccagcca tgcgccagct
     3301 tcacgaacca cttctttatt cagcgggtta gaggctgcgt aatggtccat tgtcgatttc
     3361 acgacataac catcgatacc gtacatcacc catacgccag ccagtgcgaa gcagaccagc
     3421 gtcaccagtg ccgccacctg agccgttgca cgggtacgca ggtgcagttc gcccacggta
     3481 cgcatttgca gataggttgc gccctgagta atgatcatcc ctacgctcac cacacctgcc
     3541 agcaggccga acgggttaag cagctggaag aagttaccgg tgtagtacag acgcagatat
     3601 tcatcaacgt tgaatggtac gccctgcaac aggttaccga acgctacacc aatcaccagc
     3661 ggcggaacga agctaccaat gaagatgccc cagtcccaca tgttacgcca gcgggtttct
     3721 tcaatcttgg agcggtagtc aaaaccgacc ggacggaaga acaaagacgc cagcacgagg
     3781 atcatcgcca catagaagcc ggagaacgca gcggcataga ccatcggcca ggcagcaaac
     3841 agtgcgccgc ctgcggtgat cagccaaacc tggttaccgt cccagtgtgg tgcgatggag
     3901 ttaatcataa ttcgacgctc ggtgtcgtta cgaccgagga aacgggtgag catgcccacc
     3961 cccatgtcga aaccgtcagt gactgcaaaa ccaattagca gaacgccaac cagcagccac
     4021 cagataaaac gcaatacttc ataatcgatc atttgacgac tcctgtctta gcgtgccggc
     4081 tgagtagtcg tggaagactg ctcaaagtga tagcgaccgg ttttcaggct gcttgggccg
     4141 aggcgtgcaa acttgaacat taagaacaat tctgccacca ggaacagggt atacaggccg
     4201 caaatcagca ccatcgagaa gatgagatcg cctgcggtca gtgacgagtt cgccacagct
     4261 gtcggcagca cttcaccgat agcccacggt tggcggccat attcagccac gaaccagccc
     4321 gcttctacag caatccacgg cagcggaata ccgtacagcg cggcgcgcag cagccatttt
     4381 ttctcgccaa tgcggttgcg gatgacactc cagaaagaga gcgcaatgat tgccagcagc
     4441 aggaagccac acgccaccat gatacggaac gcaaagtaca gcggcgctac acgcggaatg
     4501 gagtctttgg ttgcctgttg aatctgagct tcagtcgcat cagccacgtt tggcgtatag
     4561 cgtttcagca gcagaccgta accgagatct ttcttcatgc tattgaactg gtcacgaacg
     4621 gcctggtcag tagaaccaga acgcagttgt tcgagcagag agtacgcctt catcccgtta
     4681 cgaatgcgtt cttcatgctg caccatcagt tctttcaggc cgataactgg agtatccacg
     4741 gaacgcgttg caatgatgcc cagcgcgtaa gggatctgga tcgcaaattt gttcgtctcc
     4801 tcttcctgat caggaatgcc gaacagagta aaggcagcag gtgcaggttg cgtttcccac
     4861 tcggcttcaa tagcagccag tttggttttc tgcacgtcgc ccatttcgta accggattca
     4921 tcacccagaa caataacaga cagaacggca gccataccga agctggcagc gatagcaaag
     4981 gagcgtttag cgaaggcgaa gtcacgacct ttcagcatat accatgcgct gataccgagg
     5041 atgaacatcg cgccagtcac ataaccagaa gctacggtgt gaacgaattt cacctgagca
     5101 accgggttaa gcaccagctc ggagaagctc accatctcca tacgcatagt ttcaaagttg
     5161 aaatcggacg cgattgggtt ttgcatccag ccgtttgcaa ccagaatcca cagtgcggac
     5221 aggtttgaac cgagcgccac cagccaggtg acacacatat gctgaacttt acccagacga
     5281 tcccaaccga agaagaacag acctacaaag gtggattcga ggaagaaggc catcagacct
     5341 tcgattgcca gcggcgcacc gaagatatcc cctacatagt gggaatagta agaccagtta
     5401 gtcccgaact ggaactccat ggtcagaccg gtagccacac ccagagcaaa gttgatacca
     5461 aacaacttgc cccagaactt ggtcatatct ttataaatct gtttgccgga aaggacgtag
     5521 accgtttcca taatggccag caggaacgcc ataccgagcg tcagtggcac aaaaaggaag
     5581 tggtacatcg cggtcaaggc aaactgtaag cgcgacagtt cgactatatc taacatcatg
     5641 actccttgct catcgcatga agactccgag agtgaacccc gttagaaagg gtcacacgca
     5701 tgccccaata caaaattatt tgctcgcctc tatcgttgcc atttcatcaa aagaggaaaa
     5761 gacaatgata aa
//
LOCUS       DAZFJL010000195         5692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFJL010000195 DAZFJL010000000
VERSION     DAZFJL010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.197"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            157..807
                     /gene="csgD"
                     /locus_tag="ACHJ8C_004374"
     CDS             157..807
                     /gene="csgD"
                     /locus_tag="ACHJ8C_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO5554128.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGILRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            812..1201
                     /gene="csgE"
                     /locus_tag="ACHJ8C_004375"
     CDS             812..1201
                     /gene="csgE"
                     /locus_tag="ACHJ8C_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO5554129.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1226..1642
                     /gene="csgF"
                     /locus_tag="ACHJ8C_004376"
     CDS             1226..1642
                     /gene="csgF"
                     /locus_tag="ACHJ8C_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO5554130.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1669..2502
                     /gene="csgG"
                     /locus_tag="ACHJ8C_004377"
     CDS             1669..2502
                     /gene="csgG"
                     /locus_tag="ACHJ8C_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO5554131.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2566..3057)
                     /gene="ycdZ"
                     /locus_tag="ACHJ8C_004378"
     CDS             complement(2566..3057)
                     /gene="ycdZ"
                     /locus_tag="ACHJ8C_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO5554132.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYAITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQQIK
                     NKA"
     gene            complement(3159..3713)
                     /gene="ycdY"
                     /locus_tag="ACHJ8C_004379"
     CDS             complement(3159..3713)
                     /gene="ycdY"
                     /locus_tag="ACHJ8C_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO5554133.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKVAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAALTRDAISAMWDELEEDSEE"
     gene            complement(3737..4474)
                     /gene="ycdX"
                     /locus_tag="ACHJ8C_004380"
     CDS             complement(3737..4474)
                     /gene="ycdX"
                     /locus_tag="ACHJ8C_004380"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO5554134.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLELIIAGFHE
                     PVFAPHDKATNTKAMIATIASGNVHIISHPGNPRYPIDFKAVSEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAIAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4529..5467)
                     /gene="ghrA"
                     /locus_tag="ACHJ8C_004381"
     CDS             complement(4529..5467)
                     /gene="ghrA"
                     /locus_tag="ACHJ8C_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO5554135.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVVQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY"
BASE COUNT         1552 a         1396 c         1303 g         1441 t
ORIGIN      
        1 attgaaatca gatgtaatcc attagtttta tattttaccc atttagggct gatttattac
       61 tacacacagc agtgcaacat ctgtcagtac ttctggtgct tctattttag aggcagctgt
      121 caggtatgcg atcaataaaa aaagcggggt ttcatcatgt ttaatgaagt ccatagtatt
      181 catggtcata cattattgtt gatcactaaa ccttctttgc aggcgacagc tctcttgcag
      241 caccttaaac aatcgctggc gattacagga aaattacata atattcaacg ttctctggac
      301 gatatatctt caggctctat tattcttctg gatatgatgg aagcggataa aaagcttatc
      361 cattattggc aggatacttt gagcaggaaa aacaacaata tcaaaatatt gttgctaaat
      421 acgcctgaag attacccgta ccgcgacatt gaaaactggc ctcatatcaa cggcgttttt
      481 tatgccatgg aggatcaaga acgtgttgtc aatgggttgc aaggcatcct gcgcggcgaa
      541 tgctacttta cgcaaaagct tgccagttac ctgattacgc attcaggtaa ctatcgttat
      601 aacagcacgg aatcagccct ccttactcat cgggaaaaag agatcctgaa taagctgcgt
      661 atcggcgcat ctaataacga gatcgctcgt tcgttgttca tcagcgaaaa tacggttaaa
      721 acgcatcttt ataatctttt caagaagata gccgtaaaaa accggacaca agcggtttcc
      781 tgggcaaacg ataacctcag gcgataaagc catgaaacgt tatttacgct ggattgtggc
      841 ggcagaattt ctgttcgccg cagggaatct tcacgccgtt gaggtagaag tcccgggatt
      901 gttaactgac catactgttt catctattgg ccatgatttt taccgagcct ttagtgataa
      961 atgggaaagt gactatacgg gtaacttaac gattaatgaa aggcccagtg cacgatgggg
     1021 aagctggatc acaataacgg tgaatcagga cgttattttc cagacttttt tatttccgtt
     1081 gaaaagagac ttcgagaaaa ctgtcgtctt tgcactgatt caaactgaag aagcactaaa
     1141 tcgtcgccag ataaaccagg cgttattaag tacgggcgat ttggcgcatg atgaatttta
     1201 aataaaaaat tgttcggagg ctgcaatgcg tgtcaaacat gcagtagttc tactcatgct
     1261 tatttcgcca ttaagttggg ctggaaccat gactttccag ttccgtaatc caaactttgg
     1321 tggtaaccca aataatggcg cttttttgtt aaatagcgct caggctcaaa actcttataa
     1381 agatccgagc tataacgatg actttggtat tgaaacgccc tcggcgttag ataactttac
     1441 tcaggccatc cagtcacaaa ttttgggtgg gctactgtcg aatattaata ccggtaaacc
     1501 gggccgcatg gtgaccaacg attatattgt tgatattgct aaccgcgatg gtcaattgca
     1561 gttgaacgtg acagatcgta aaaccggaca aacctcgacc atccaggttt cgggtttaca
     1621 aaataactca accgattttt aagccccagc ttcataagga aaataatcat gcagcgctta
     1681 tttcttttgg ttgccgtcat gttactgagc ggatgcttaa ccgccccgcc taaagaagcc
     1741 gccagaccga cattaatgcc tcgtgctcag agctacaaag atttgaccca tctgccagcg
     1801 ccgacgggta agatctttgt ttcggtatac aacattcagg acgaaaccgg gcaatttaaa
     1861 ccctacccgg caagtaactt ctccactgct gttccgcaaa gcgccacggc aatgctggtc
     1921 acggcattga aagattctcg ctggtttata ccgctggagc gccagggctt acaaaatctg
     1981 cttaacgagc gcaagattat tcgtgcggca caagaaaacg gcacagttgc cattaataac
     2041 cgaatcccgc tgcaatcttt aacggcggca aatatcatgg ttgaaggttc gattatcggt
     2101 tatgaaagca acgtcaaatc gggcggggtt ggggcaagat attttgggat cggtgccgac
     2161 acgcaatacc agctcgatca gattgccgtg aacctgcgcg tcgtcaatgt gagtaccggt
     2221 gagatcctct cctcggtgaa caccagtaag acgatacttt cttatgaagt tcaggccggg
     2281 gttttccgct ttattgacta ccagcgcttg ctggaagggg aagtgggtta cacctcgaac
     2341 gaacctgtta tgctgtgcct gatgtcggct atcgaaacag gggtcatttt cctgattaat
     2401 gatggtatcg accgtggtct gtgggatttg caaaataaag cagaacgaca gaatgacatt
     2461 ctggtgaaat accgccatat gtcggttcca ccggaatcct gatagataaa aaagccgaaa
     2521 ttatcatccg gcattttacg gtttaagcga aagcgtgagt gcccctcacg ctttattttt
     2581 gatttgctgc tcacggtgag ccgtctttgc actacgcgcc gccaaccaca ggccgctgtt
     2641 tttcattgcg taaccaaata ccagtccaag cgccagcgaa ggtaacacca gtttccagtc
     2701 accctgcccg gcaaacgtcg cgcatgcgcc tataaacgtc cccggaacaa atgaaagcag
     2761 cagctgtttg gcctggatac acatcagaaa cgcgacaata ccggttatgg cataaccgag
     2821 tatttccaga tgtggtgcca gtgcactacc gtaaataatg accatcgccc acaccacgcc
     2881 actaagcagg gttgcagcgg agatcgccag ccctttcagc ccaccttgcg ggcaggcaaa
     2941 gtaggccgta cagcccagga agcccgccca gcttagtaag cccagggaaa cagccaccca
     3001 tccccagata ccggagagaa tgcctgttgt gattgcaata gagagaagta tgttcatgcc
     3061 gcgcatgata gcagaaaacg cggccatgaa tgagatcgag cacacatttt atgcaacaac
     3121 aaattacctg ttgcagttat gagtgatgca gatcacattt actcttcaga atcttcttcc
     3181 agctcgtccc acattgcact aatagcatcg cgggttagcg ctgccatggt tcgccagaaa
     3241 ggcgtagttg catgggcctc cactttgcca aggaacgcac cacaccaggg taacagatac
     3301 tcactgaata gtgtttccag tgcttcgctc tcatcttccg ttgactgatc ttccagccag
     3361 gaagccgcaa gcagcaatgt gccgatgtga tcggctggcg tatccgctag tggcatccct
     3421 cgttcggaaa gaaaagcgcg cacttccgct tccgttgcgc cctcaaccca tgcgctacga
     3481 tatggcggca cagcacattc atcgccgata aacaacgcat tgtaatcggc agagacttgc
     3541 gtcatatcac agcttttctg taaacgcgtc agtaactcat cctgctccag tggccagttc
     3601 gcagccactt tcccctcacg aatcagggta aacaacggca ccagtaaagg atcttgcggt
     3661 tggcggtaat agagcgaacc cagcacacga cagaggatag aaaactcatt cattaatata
     3721 tttccattac tagtaattaa aggtctgcaa attccgcaat cggtgccata ccgcgagatt
     3781 caaggaagtt aagtaagcgg cgcggagaaa cattcaaaat gcgctctggc ggaaaatcta
     3841 ccgcgtcgag gattttaaga cactcttcaa attcccccat ggtaaacgca gtgtgagaat
     3901 ccgagcctaa tgccacccaa cctccagcgt cacgtaccgc tgcggctatc gcacgacagt
     3961 tgtcttcact gcccttacgt gagtgtaaaa atgaggaatt attgatttcc agcgccacct
     4021 gatgtttcgc tgcagcttca gaaacagctt taaaatcaat tggatatcgt ggatttccgg
     4081 gatgacttat tatatgcaca ttgccgctgg cgatagtggc gatcatcgct tttgtattgg
     4141 tcgctttgtc atgtggcgca aaaaccggct catgaaaacc ggcaataatc agctccagcg
     4201 aatcaaacat tttgccgctg cagtcaattt caccatcaac atttttaatg ttagcttcga
     4261 tgccgcgcag gatccctacc ccatcaacca ctcgcggcca gatacgcatg ttaatgaagt
     4321 gccagtgatg cggcgcatct tccatatccg ggccatgatc ggtgatcgca aaaagtttaa
     4381 tgcccttttg tttggcctgg gcaatgtaat cactcaatgt gctatatgca tgcgtgctgg
     4441 caacggtatg catatgaagg tcgacgggat acataactct ctcctgtatt caattcccgc
     4501 caaggatagc aggaaacctg atgctttatt agtagccgcg tgcgcggtcg acttgcccgc
     4561 agaccctctc ccctttttcg agctgggcaa tggtgcgcga aatgtaatcc acagcttcag
     4621 cgggacgggt aatcgcggcg acatgtggtg ttatcgtcac gcgtggatgt tgccagagcg
     4681 gactttccgg cggtaagggt tcacgattaa aaacatccag cattgcgcct ttaactttgc
     4741 cgctatccag cgccgcgagc aggtcatctt ccacgacatg aacaccacgc gccaggttga
     4801 ggagatacgc gccatccggt aatttttcga gtaattgttg attaataatg ccgacggttt
     4861 caggtgtatt cggtaacaaa ttaatcaata cccgacattg gctcagaaat gcagagagtt
     4921 cttcccgtcc ggcaaagctt tgcacgccag gccacgattt acgggttcga ctccagcaac
     4981 gcagcgggaa gcgccaggtt tgcagactct gtacaacttt actgcccagt acgcctgcac
     5041 ccaaaatgcc gatggtaaaa tcttcccgat gatattcagg cagcggttgc caatgcgaac
     5101 tattttgctg gatgcggtaa tcgtcaaaac gtcgaaacca atgcagcacc tgactgacag
     5161 catattcctg catttgctcg cccataccgg tatcttccag gcgaaaaagt ggaacagaag
     5221 gcttcagcat ttcagggtgt gcctgtagct tgctcaaaat agaatcaaca ccggccccga
     5281 gtgcgaacac cgctttaaga tcgcgccctg ccagcatttc aactggagga tgccagacta
     5341 aagcataatc agcagagtca ttatctccgc ttttccatgc tctgactctt gcctgaggaa
     5401 tagctttgcg cagtgcctca atccaccatt gggtatcgaa cgttgggtga taaaagatga
     5461 tatccatact gactcccgaa aagcgttgcg cgaaatttat tcgcacttat cgttatgatc
     5521 tacaaaagcc accagcataa caaatccgtg gtcggtggca aaaaaagcaa atttcgctta
     5581 ttaaaactgc atattgattg aagtttgaat aatcgagcga ttttttaaaa aagtttgttg
     5641 acctcaggtc atgatttccc taaattagcg cccgttccag caagacagga ac
//
LOCUS       DAZFJL010000196         5573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFJL010000196 DAZFJL010000000
VERSION     DAZFJL010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.198"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..364)
                     /locus_tag="ACHJ8C_004382"
     CDS             complement(<1..364)
                     /locus_tag="ACHJ8C_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5554136.1"
                     /translation="MEKFSLEFKKVVSEKYLEGELSLKSVARMYGISPCTVRKWSYAY
                     REHGISILTGKKGRYSAELKLTVVKEVVDDCFSVREVAVKHGIPAFGTVCNWLEKYRK
                     YGEDAFIRKNKKSIPVPDK"
     gene            743..1018
                     /locus_tag="ACHJ8C_004383"
     CDS             743..1018
                     /locus_tag="ACHJ8C_004383"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554137.1"
                     /translation="MTHALAQAHANAQKLKARQAEMQRKEDEERERERRQQNAKHSAP
                     ATDEPEYVELPRIPELSPEEQAALDKSMEKERAEMEKILGPIPTSFN"
     gene            1230..5327
                     /locus_tag="ACHJ8C_004384"
     CDS             1230..5327
                     /locus_tag="ACHJ8C_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO5554138.1"
                     /translation="MNKIYSHRICHKTGIIKVVSELISHTRKSKSKKYDRTLFLIPVI
                     FSLLSFPTGSKASVVNGNNAYQNYRDFAENKGAFQPGATDIPVYDTNGNVTGTLSISM
                     PDFSSVATEGYATLISPQYIVSVKHNKGYNTVSFGDGQNSYTIVNRNEDPSRDFHTPR
                     LNKLVTEVSPADAISTGDSSNFYTDSERYTAFVRLGSGRQFIKNEDGTLTQLAAGYSY
                     LTGGTLKAPVVYQNGNSLKATTPDLFNSDMGLLPTTAQPGDSGSPLFAWDSWTGKWVL
                     IGVQTAISSSWSYWAAIPVSDIQAIINDDSDGTISFLDSAGALIWTYNSTDGTGTISQ
                     GNTTYTMHGTKDGNLDAGKNLTFTGDDGLILLRDNVDQGAGTLTFNSNYTVSTDNNST
                     WMGGGLDIAEGTSVTWQVNGVSGDNLHKIGAGTLIVNASGINEGGLKVGDGTTVLDQQ
                     PDKDGNVQAFSSVNIASGRATVVLADSNQVDPDNISWGYKGGTLDLNGNNLTFHQIKA
                     ADYGAVIANTADSTSTVTLSYSLNSNDVNINNWAGLNSKGTPGDLYVYNNTYTNTTDY
                     FILKTDGYYGYYPTNQSSSDIWEYVGHDQDAAQKLVADRYNSSGYLYHGQFTGNLNIV
                     NTVASGATGSFVMDGSADISGSFTQENGRLTLQGHPVIHAYNSQSIADTVASTGDDSV
                     LTQPTSFTQSDWENRSFTFGALNLKNTDFSLGRNATLNTVIQAENSVVTLGGARVYID
                     LKDGDGTDFELEEGESIATADADKSHFYGSVILDSNSTLTVNEVFNGNIQASDSSVTI
                     NSDEVLIGDVVLQNSAMTLNDGASAVVSDSLNTDGAVSVDNAFLQLGENGNTRTRSSA
                     GVYRAAQINLSGEKAALYVTEGNTLYSDILSDSSASLIFGDSTQALSGTSDGTQVTRW
                     EGSLNAEDASLTLYGTEWRVTSDSQSGTLSARNSLIDLSSYSDGSRRLSVSNFDSSDS
                     TYRLNVGSGYNDQLVVTESATGQNNTLLLNFTSQPQTGDVKQTLVIAPDSTSSDLFHV
                     STISNGFSEFIPETTVDTTDGVKKWELESIQVQENKPVTQAAGYFLRSGYQQFLTEMN
                     NLTIHMSDLRDNDAPTGVWARLINGSGSGKGDYSDRYTHFQMGADRKHELESMDLIHG
                     VMLTYTDSRADGKQYTGKTRSYGAGIYASGIFPDGAYFDLTGKYVQHANDYTLSLAGA
                     GQQTFHSHSLYAALQVGQRYRYGGNVWLEPQAELVYGRISGRNFSWSDNSIPLRMKSS
                     DMTPLVGRIGIESGKDIDMKKWSARLRAGVSYQADLRDHATVLLQDSASTYKSTSGKD
                     NRVLLNAGLQMNLGQDKRLDMGLEKSFMGDYNVDHAVKASFTWSF"
BASE COUNT         1552 a         1333 c         1324 g         1364 t
ORIGIN      
        1 ttttatccgg cacaggaata ctctttttat tttttcgaat gaaagcatct tcgccatatt
       61 ttctatattt ttcaagccag ttgcatacag taccaaaggc tggtattccg tgttttaccg
      121 ctacttcacg aacagaaaaa cagtcatcca cgacttcttt tactactgtg agttttaact
      181 cagcagaata gcgtcctttt ttacccgtaa gtatgcttat cccatgttcc cgataggcat
      241 aagaccattt tcttactgtg cagggactga taccgtacat ccttgctaca gatttcagac
      301 tcagctctcc ctccaggtac ttttctgata ctaccttttt gaactccaat gaaaactttt
      361 ccatgcatcc tcagtttttg gtctggtgtc taacctttgg ggcgcacttc aaaaatgatg
      421 gacattattt ttgtggagcc ggaggaaaca gaccagacgg tttaaatgag ccggttacga
      481 aaaaacaacc gctactctgg ataatgatga ccaccgtact gcagtccggc tgcacaggca
      541 acagggacca tcacccgcag gtgacattca cggttgatac cgtgcgaacg gacacgacgg
      601 ccagcaatcc tgtatgcctg ccggaaaaat acgggagcct tcttaatgag cgccatccgg
      661 acctgtttgc cggtgctcag gggattatta caaagggggt gcctataatg cagacagcag
      721 aacatctaca cctgcacgcc caatgactca tgccctcgca caggcccacg ccaacgcgca
      781 aaaacttaaa gccaggcagg cggaaatgca gcgcaaggaa gatgaggagc gcgagcgtga
      841 acgccggcag cagaatgcaa aacattcagc accggcaacg gatgaaccgg aatacgttga
      901 actgccacga atccctgaac tttcgccgga agagcaggca gcactggaca aatcgatgga
      961 aaaagagcgc gcggagatgg agaaaattct gggaccaatc cccacctcct tcaattaaga
     1021 ctctccacct gccgtctctc tcagggcacc gtatggtgcc ctgaatgttt ttccctaacg
     1081 tcagagaacg cccttcccct gcacctgagc ggttaagtgc accagactta cctttgtatt
     1141 gattttcatt gattaatcaa tagaataagc ttcccccagg gaagtcacct cctgtacttt
     1201 tttattatgc acataactac acttcagata tgaataagat atatagtcac cgtatttgcc
     1261 acaagaccgg tattattaaa gtcgtttcag aacttatatc tcatacgcga aaatcaaaaa
     1321 gcaaaaaata cgacaggaca ttatttttaa ttcctgtcat tttcagtcta ttatcctttc
     1381 cgacagggag taaagcgtcc gttgtaaacg gaaataatgc atatcagaac tacagggatt
     1441 tcgctgaaaa taagggggcc tttcagcccg gagcaactga catccctgtt tatgacacaa
     1501 atggcaacgt gactggcacc ctttccatct caatgcctga tttcagtagt gttgcgacag
     1561 aaggatatgc cacgctgatt tccccacaat atattgtcag cgttaagcat aacaaaggat
     1621 acaatacggt aagttttggc gacggtcaga actcatacac gattgttaat cgcaacgaag
     1681 atccatccag agattttcat acgccacgac tgaataaact ggttaccgag gtttctcccg
     1741 ccgacgctat ttcaacggga gacagcagca atttttatac cgattctgaa aggtatacag
     1801 ccttcgttcg cctcggctcc ggtcggcaat ttatcaaaaa tgaggacggt acactgacac
     1861 agctggctgc agggtacagt tatctgaccg ggggaaccct gaaagcaccg gtggtgtatc
     1921 agaatggtaa ctctctcaaa gcaaccaccc ctgatttatt caattccgat atgggactgt
     1981 tacccacaac agcccagccc ggtgacagcg gctcaccgtt atttgcatgg gacagctgga
     2041 caggaaaatg ggtcctcatt ggtgtgcaga cggccatcag ttcaagctgg tcgtactggg
     2101 cggcgatccc cgtatctgat attcaggcca ttataaatga cgactccgat ggtacgattt
     2161 cctttcttga ctctgcaggt gcgctgatat ggacatataa cagcactgac ggcactggca
     2221 cgatttcaca gggcaatacg acctatacca tgcatggaac gaaagacggc aatctcgatg
     2281 cgggtaaaaa ccttacattt accggagacg atggcctaat cctgctcagg gataatgtgg
     2341 atcagggtgc cggcacgctg acatttaaca gtaactacac ggtgagcact gacaataatt
     2401 ccacatggat gggaggcggt cttgatatcg ctgagggcac ctccgtcacc tggcaggtca
     2461 atggtgtcag cggtgataac cttcataaaa tcggagcggg aacattaatt gtcaatgctt
     2521 cagggataaa tgaaggcggc cttaaagtgg gcgatggcac caccgtactt gatcaacaac
     2581 ctgacaagga cggaaatgtt caggcattca gtagtgtcaa tatcgcaagt ggcagggcaa
     2641 ctgttgtgct tgcggacagt aatcaggttg atcctgataa tatctcatgg gggtacaagg
     2701 gaggaacgct ggatctcaac gggaataacc tgacatttca tcagataaaa gctgctgact
     2761 atggtgccgt tatcgcaaat actgcagaca gtacatccac agtgactctg agttattctc
     2821 tgaacagcaa tgatgtaaat attaataact gggccggcct gaacagtaaa ggaacccccg
     2881 gtgatttgta tgtttataac aacacttata ctaatacgac agactatttc attctgaaaa
     2941 ctgacggata ttacggctat tacccgacga accagagcag ctctgatata tgggagtatg
     3001 tgggacacga ccaggatgca gcgcagaaac tggttgcaga cagatataat tcatccggtt
     3061 atctttatca tgggcagttt accgggaatt taaatattgt taacaccgtt gcatccggag
     3121 caaccggttc attcgtgatg gatggctctg ctgacatctc aggctctttt actcaggaaa
     3181 atggtcggct gaccctgcag ggacacccgg tgatccatgc ttataacagc cagagtatag
     3241 ctgatacggt tgcctctacc ggagacgact ccgtcctgac tcagccgaca tcattcacac
     3301 agagtgactg ggaaaaccgg agttttacgt ttggagccct gaatctgaaa aatacagatt
     3361 tcagtctggg gcgtaatgcc acactgaata ccgttatcca ggctgaaaac tcagtggtca
     3421 ctctgggtgg cgcacgtgtt tatattgatt taaaagatgg cgatgggact gacttcgagc
     3481 ttgaagaggg cgaatccatt gccacggcag acgcagacaa gagtcatttc tatggttctg
     3541 tcattcttga cagtaactcc accctgacgg tcaatgaagt ttttaacggc aatattcaag
     3601 cttcagacag tagtgtcacg ataaactccg atgaggtact tattggtgac gttgtattac
     3661 agaactctgc aatgaccctg aatgatggtg catccgcagt ggtctccgac agtctgaaca
     3721 ctgatggtgc tgtttctgtt gataatgcct ttctgcaact cggcgaaaac ggcaataccc
     3781 ggacccgttc ttcagccggt gtatacaggg cagctcagat taatctgtca ggagaaaagg
     3841 ccgcactgta cgtcacggaa ggaaacacgt tgtacagtga tattctgtca gacagctctg
     3901 catctctcat ttttggagac agcacacagg cattatccgg cacctctgat ggcactcagg
     3961 taacccggtg ggaaggctca ctgaatgctg aggatgccag cctaacgctc tacggaaccg
     4021 aatggcgcgt gaccagtgat tctcagtctg gaaccctgtc agcgaggaac tcgctgattg
     4081 acctgtcatc ttactctgac ggaagcagac gtctttctgt cagtaatttc gacagcagtg
     4141 acagcacata ccgccttaat gtgggaagcg gatataacga tcagcttgtg gtgacagaaa
     4201 gcgccacggg tcagaataat acactgttgc tgaacttcac ttcacaaccc cagacgggag
     4261 atgtaaagca gacgctggta atagcgccag acagcacatc atcagacctt tttcatgtgt
     4321 ctaccatcag caatggtttc agcgaattca tacctgaaac cacggtcgac acaactgatg
     4381 gtgtgaaaaa atgggaactc gaaagtatac aggtgcagga aaataaaccc gtcactcagg
     4441 ctgcaggtta ttttctgagg agtggttatc agcagtttct gacagaaatg aataacctga
     4501 caatacatat gagcgatctc agagataatg atgctccgac cggcgtctgg gcccggctga
     4561 ttaatggaag cggctcaggc aaaggggact acagcgatcg ctatacccat ttccagatgg
     4621 gggcagacag gaaacatgaa ctagagagca tggatcttat tcatggtgtt atgctgacat
     4681 atacagacag ccgtgctgat ggaaaacaat ataccgggaa gacccgttca tatggtgccg
     4741 gtatctatgc cagcgggata tttccggatg gcgcatactt tgacctcacc ggaaaatatg
     4801 ttcagcatgc gaatgactat accttatccc tggccggggc ggggcaacag acattccaca
     4861 gccattcatt gtacgctgca ttacaggtag gacaacgtta tcgctatggt ggaaatgtct
     4921 ggcttgagcc tcaggctgaa ctggtttatg gcaggatctc cggcagaaac ttcagttgga
     4981 gtgataacag catcccccta aggatgaaaa gctctgacat gactcctctg gtcggacgaa
     5041 tcggtattga aagcggcaag gacattgaca tgaaaaaatg gtctgctcgg ttacgagcag
     5101 gtgtcagtta ccaggccgat ttacgcgatc atgccacagt actgctacag gatagcgcca
     5161 gtacatataa atccacctca ggcaaagata accgggtgtt gctgaatgca ggtttacaga
     5221 tgaatctggg tcaggacaaa cgcctggata tgggactgga gaaatcattt atgggtgact
     5281 ataacgttga tcatgctgta aaagcatctt ttacctggtc attctgacga tacagctccg
     5341 gaaggacaga agagcgctga agcctctgtc atcactctca gggcaccgcc tggtgccctc
     5401 tgcaattatt aaagggcagg aagcggtcac ctcctgcccc tggttaaaaa agaggtttca
     5461 aatatcaacg taaccgtctc atttaaacgt aaccggctca tttaaaccgt ctggtctgtt
     5521 tcctccggct ccacaaaaat aatgtccatc attttttgaa gtgcgcccca aag
//
LOCUS       DAZFJL010000197         5432 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFJL010000197 DAZFJL010000000
VERSION     DAZFJL010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.199"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            20..81
                     /locus_tag="ACHJ8C_004385"
                     /pseudo
     CDS             20..81
                     /locus_tag="ACHJ8C_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(171..605)
                     /gene="uspA"
                     /locus_tag="ACHJ8C_004386"
     CDS             complement(171..605)
                     /gene="uspA"
                     /locus_tag="ACHJ8C_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO5554139.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            996..1331
                     /gene="uspB"
                     /locus_tag="ACHJ8C_004387"
     CDS             996..1331
                     /gene="uspB"
                     /locus_tag="ACHJ8C_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO5554140.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(1402..2901)
                     /gene="pitA"
                     /locus_tag="ACHJ8C_004388"
     CDS             complement(1402..2901)
                     /gene="pitA"
                     /locus_tag="ACHJ8C_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO5554141.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            3133..4326
                     /gene="yhiN"
                     /locus_tag="ACHJ8C_004389"
     CDS             3133..4326
                     /gene="yhiN"
                     /locus_tag="ACHJ8C_004389"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO5554142.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVAFRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDLSLKQLNVRDQQTLISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLVAE"
     gene            complement(4413..>5432)
                     /gene="yhiM"
                     /locus_tag="ACHJ8C_004390"
     CDS             complement(4413..>5432)
                     /gene="yhiM"
                     /locus_tag="ACHJ8C_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HFO5554143.1"
                     /translation="IPLLMGIICIALGVFVLTSSGQSEYFVAGHVLISLSAICLALFT
                     TAFIIISQLTHGMNKFYNRLFPVIGYAGSATTMIWGWSLLASNNVMADEFVAGHVIFG
                     VGMIAACVSTVAASSGHFLLIPKNASGSKSDGTPLQAYSSTIGNCLIAVPVLLTLFGF
                     IWSVILLRSADITPHYVAGHVLMGLTAICACLIGLVATIVHQTRNTFSVKEHWLWCYW
                     VILLGSLTIIFGIYVLISSDASARLAPGIILICLGMICYSIFSKVWLLALVWRRTCSL
                     ANRIPMIPVFTCLFCLFLAAFLAEIAQTDMAYFIPSRVLVGLGAVCFTLFSIVSILEA
                     GSAKK"
BASE COUNT         1449 a         1347 c         1384 g         1252 t
ORIGIN      
        1 actaatgata gaaaatataa tggtgttaaa tgttgtattg gcaggggttt tgattgcgta
       61 cgcagaccgc aggccagata aggcgtttac gctgcatcag gtaacaaggc gcgccaaaaa
      121 gcaaaacgcc cgcatatgcg ggcgttcagg aacacgtcgt ggagggaaga ttattcttct
      181 tcgtcgcgca gcggaacaat cagcatatca acgtgaacgg tgttgatcag ctgacgtgcg
      241 gaagacatca gtttgctcca gaagtcctgg tggtgaccac aaaccaccag atccatatcg
      301 tatttcttga ttgcatcgac cagtacctgg cccagatcgc cgctgccgct cagggtttca
      361 gtgattgggt agcctgcatt ggttgaaagc tcggtcagcg catgatgtgt ctcttcagag
      421 atgcgtttct gcatatcacc cagattcaca tcgataagcc cggtgtatag gtcagagtag
      481 tttacatcta cgtggatcag agaaactttc gcattgtagg ggcgagccat agagactgct
      541 ttctctacca gaactttgct ttccggggag aggtcgaccg cgatgagaat gtgtttataa
      601 gccatagtgt tactccttcc ataaagttgt cgatgactgg ccagctagcg tttcttgtgc
      661 cgttacgtca cccgcgtcct gcgaactacg tgcgcggggc taacccagcc gtaaagattc
      721 agtgttaaga ccatccttac cttatagcga cccggatgat ccgtcaatcc gccttgctta
      781 ctcgttagat aaacaaattt tcgacatgaa tatattgata gtggttaacc ttctggaaaa
      841 aaaacaacct gatctcctac actatctata gagccgctcg gatgttggtc accacaaaag
      901 cggtttctcc aggacctggt gttaactgat tggcagcgta tcgggagcgg tagccccggg
      961 gtggaactcc gcgggcaggt cgccggggag gaggtatgat aagcaccgtc gcattatttt
     1021 gggctttatg tgtcgtttgc attgttaaca tggcgcgcta tttctcatca ctacgcgcgt
     1081 tgttagtggt actgcgtaac tgcgatccat tgctctatca atatgttgat ggagggggct
     1141 tttttacctc acatggccaa cccaacaaac aggtgcgtct cgtttggtat atctatgccc
     1201 aacgttatcg cgatcatcac gatgatgagt ttattcgccg ctgtgagcgg gtgcgtcggc
     1261 agtttattct gaccagcgca ttgtgtggtc tggtggtggt cagcctgatt gcattgatga
     1321 tttggcatta agcacttcag caataatgac gcataaaaaa agcgggccag taaactgacc
     1381 cgctcgtttt ggtgcgtacg attacaggaa ctgcaaggag agccagtaca gcccgccgga
     1441 aagcagtacc gcagccggaa gggtaaatac ccaggccatc agaatactgg ttacggtttt
     1501 acgctgtaag ccgccgccat ctaccaccat cgtccccgcg acagaagagg agagtacgtg
     1561 agtagtggaa accggcatcc cggtataact tgccaggccg atagacactg ccgccgtcat
     1621 ctgggcagac atcccctgag cgtaggtcat gcctttctta ccgattttct caccgatagt
     1681 cgttgccaca cgacgccagc caatcatcgt accgatacct aacgccagcg cgaccgccat
     1741 gatgatccac accggcgcat actcgatggt gctaagcatg tcggacttca gtttcttcaa
     1801 caggcgctga tcgtcagcac tcacgccagg catcttcacc actttgtcga tagtgtcaga
     1861 aacgcacagc ataatgcggc gcatctggct acgttgatca agcgacagct tgtcgtagct
     1921 ttccacatcg gtagtcaaca tgcctttcag gcggttgagc gcgttaatgg tattcgacgg
     1981 atggcaatgg aactccgcgg gttgcgttgc gccagcttcc ggagccggta ctaactgatc
     2041 agcaccggtc gcttgtttca gcagcgcagg atgctgctca aagtaagctt cgacgttgtt
     2101 gatggcatca cgggtacggg tgatttcgta gccagtggca ttcatgttca ccacgaagcc
     2161 tgctggtgcg acaccaatca ataccaacat aaccagacca atgcctttct gaccatcgtt
     2221 cgcgccgtgc gaaaacgcca cgccgatagc ggaaaggatc agcgcaatac gcgtccagaa
     2281 cggcggcttt ttcttgccgt ctttcttttc acgctccgct ggggtcaggt ggatacgggc
     2341 gcgtttcttg gtgccgctcc agtagcgacg cagcaagaaa atcagaccgc cagcaaacac
     2401 caggccgaca ataggggaaa cgatcagaga accgaaaata cttaatactt tcgggatatt
     2461 gagtgcatcc accactgacg tcccggtcat caacgcattg gttaaaccaa tcccgatgat
     2521 cgcgccaatc agcgtatgag agctggatgc aggtaaacca aagtaccagg tacccaggtt
     2581 ccagataatc gccgccagca acatagagaa caccatggca aggccatgag acgatcccat
     2641 attaagcagc agatccgtcg gcagcatatg cacaatggca taggcaacac tcagaccacc
     2701 cagcaaaaca cccaaaaagt tgaataccgc cgccataacc acggcgagct gagaacgcat
     2761 cgcgcgggta tagataacgg ttgccacggc gttggctgtg tcatggaaac cattgatggc
     2821 ttcgtagaac agcacaaaag ccagtgcaag caataataac agcccggtat gcaaatccag
     2881 gccagcaaac aaatgtagca taggacgtta cgccattttg aggacatgaa cgcggcgcat
     2941 tatcagtgac tttcgcggcg cgggcaaagt gaaatataga ctttttttga tttgcctcct
     3001 gtatggatta cactcaaaaa ataattatct tatataattc aggcaaatac ttccttttag
     3061 taatattgat gctggtgcga ccactgagga atctttacaa ttcacgcccg ttttttctta
     3121 gaggagcgca acgtggaaag gtttgatgcc attattatag gcgctggtgc ggcagggatg
     3181 ttctgttctg cgttggcagg tcaggcagga cgccgggttc tgctgatcga taatggtaaa
     3241 aaaccagggc gcaaaatcct catgtctggc ggtgggcgct gcaactttac caacctttat
     3301 gtcgaaccag gcgcttatct gagccagaat ccgcattttt gtaagtctgc actcgcgcgt
     3361 tttacccagt gggatttcat tgatctggtc aataaacacg gcatcgcctg gcacgagaaa
     3421 acgttagggc agctcttctg cgatgactcc gcgcagcaga ttgtcgacat gctggtggat
     3481 gagtgcgaga agggcaatgt ggccttcaga ttgcgtagcg aagtgctgag cgtggcgaag
     3541 gatgatacag gcttcacgct tgaactgaac ggcatgactg tcggttgcga aaagctggtc
     3601 attgcgaccg gtggactgtc aatgccgggg ctgggcgcat cgccgtttgg ttataagatt
     3661 gccgaacaat tcggcctcaa cgtgctgccg acccgtgcgg gtctggtgcc attcactctg
     3721 cataaaccgt tgcttgaaga gttacaggtg ctggcgggcg tggcggtgcc ttccgtgatt
     3781 accgctgaaa acggtaccgt tttccgtgag aacttactct tcacccaccg cggcttatct
     3841 ggaccggcgg tgttgcagat ttccagctac tggcaaccgg gtgaatttgt cagcatcaat
     3901 ctgctaccgg atgtggacct cgaaaccttc ctgaatgagc agcgtaatgc acatccgaat
     3961 caaagcctga aaaacacgct ggcggttcat ctgccgaagc ggttggttga acggttacaa
     4021 caactcgggc aaatcccgga tctctcgcta aaacagctca acgtgcgtga ccaacagaca
     4081 ctgattagca cattgactga ctggcgcgta caacccaacg gcactgaagg ctatcgcact
     4141 gccgaagtga cgctcggcgg cgtggacacc aacgaactct cttcacggac gatggaagcg
     4201 cgcaaagtgc ctgggctgta cttcatcggc gaagtgatgg acgtcaccgg ctggctgggg
     4261 ggctataact tccagtgggc gtggtcgagt gcatgggctt gtgcgcagga tttggtggca
     4321 gaataagaag caaaatgcgc tctcgatacc agagggatta acctgaataa aaccagataa
     4381 ggcgctcaac gccttatctg aagccttttt tattattttt tcgctgaacc cgcttctaat
     4441 atagaaacga tggaaaacag tgtaaaacaa actgcaccta agccaaccag aactcgcgaa
     4501 ggaataaagt aagccatatc ggtttgcgca atttcagcca ggaacgcagc aaggaacaga
     4561 caaaacagac aggtaaatac cggaatcatg ggtattctgt tggctaacga acatgtgcgt
     4621 cgccatacca gcgctaacaa ccacactttg gagaaaatac tgtagcagat catccccagg
     4681 caaatgagaa taatgccggg ggccaggcgg gcgcttgcat ctgaactgat taatacgtag
     4741 ataccaaaaa taatggtaag tgagccaagc aaaataaccc aatagcacca cagccaatgt
     4801 tctttcactg agaacgtatt ccgcgtttga tggacaatag tggcgacaag gccaataaga
     4861 caagcgcaaa ttgccgttaa ccccattagt acgtgacctg caacataatg tggtgtgata
     4921 tctgcactac gtaacagtat gactgaccaa atgaaaccaa agagagtaag cagaacggga
     4981 acggcaatta agcaattacc gattgttgaa gaataggcct gcaatggcgt accgtcactc
     5041 ttactgcccg aggcgttttt ggggatcaac agaaaatgtc cggaagatgc cgctacggtt
     5101 gatacacaag cggcaatcat gccaacgccg aaaataacat gaccggcgac aaattcatct
     5161 gccatcacat tattgcttgc caacagtgac catccccaaa tcatagttgt agcagaaccg
     5221 gcataaccaa taacaggaaa taacctgttg tagaatttat tcatgccgtg agttaactga
     5281 gatataataa taaatgcggt agtgaacaat gccagacata tcgcagaaag agaaatcaga
     5341 acatgccctg caacgaaata ttcactttgc ccagaactgg ttaatacaaa cacacctaat
     5401 gcgatgcata ttatacccat cagcaaagga at
//
LOCUS       DAZFJL010000198         5422 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZFJL010000198 DAZFJL010000000
VERSION     DAZFJL010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.200"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(91..204)
                     /locus_tag="ACHJ8C_004391"
                     /pseudo
     CDS             complement(91..204)
                     /locus_tag="ACHJ8C_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752107.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
     gene            203..408
                     /locus_tag="ACHJ8C_004392"
                     /pseudo
     CDS             203..408
                     /locus_tag="ACHJ8C_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(583..4080)
                     /locus_tag="ACHJ8C_004393"
     CDS             complement(583..4080)
                     /locus_tag="ACHJ8C_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO5554144.1"
                     /translation="MNKNIRILQFLVSILYSVQSHFSGAQTIQLNGNGIPESITRSIT
                     GVDGNAALNISVPYKTSYTQNILSVESSINIKGGTSNTSIGGAGVYGENFTLNNNGSV
                     WGGDGYNGGVAVSGNKISINNYRNVYGGNGLGGSGSSGGAGLSGDDIIVDNYRSIYGG
                     DDLGGTGGSGVTGNNITVHNSGGIWGGNGVNGGDGINGSNLFITNDNMISGGYGIKQG
                     GDAISGNQITLNNNGIVQGGYGPDGSCSVYGEDIHINNHGNISGSYNSQKDAYNTSII
                     FSGGYNSLDIYSDSVINGDIKLASIPVNGTNELIIKNINNATAINGGLMIGNGSSVYL
                     SGKNIIFNGNISIDEDASMNLSAGNANVHANTITLKSDSWLNIDTSIKNWTQDYYTLL
                     SSDTGISIADNSHIVQYNVLLTEGAESYVYTSLNDDDNKLISMLRWNNTKGMGYGTFN
                     IEKDATLNIGVSLSDNLSPLLYDGWDGKSLTKSGNGTLILSATNNYTGNTEVKSGVLI
                     LAAPDALGRTEYLYLSRGAELDMNGYPQTISKLLTAAGSVLNIHGGSLILNNGGESAG
                     TIAGDGSLNINGGMLDITGNNRYFSGVFTVNKGAQLAVSTADNLGTAFVDNYGTLTLN
                     STSAWQLTNNISGYGNVRKTGAGALNISDNAKWTGMTDIIQGTLILGNADSPVMLGSN
                     QVIVEEQGKLSGFGGVAGNLSNSGIVDLTTYMPGNILTVGGNYTGRNGLILLQTETGG
                     DNSKTDRLVIKGNASGRTRVAVTQAGGTGAETLNGIEVIHVSGNADNAEFIQTERITA
                     GAYDYILKRGQGINSTNWYLISRKDIPVPQPEAVPENHDNNLRPEAGSYVASIAAANN
                     LFVTNLYERQGQELYISHMPGEENEAGIWMYNKGKHNRWRDNSSQLRTRGNSYVVLIG
                     GDIAQWSLNGTDRWHTGMMAGYGHNHNSTNALSTGYHSEGRMNGYTAGLYATWYANDE
                     THNGSYLDSWLQYSWFDNHINGERLPAESWKSKGFTVSLEAGYSWKAGEFTDNYKGSH
                     EWYVQPQLQVVRMNVKSDKYHESNGTSIENTGNGNILTRLGARTWLTSKNGKNTRYEV
                     PFRPFVEAHWLHNSRVFGTSMNGVSIYQDGARDIGEINGGVAGMITPEVAFRADAGIQ
                     LGVHGYHNTSAMLSVEYRF"
     gene            complement(4668..4913)
                     /locus_tag="ACHJ8C_004394"
                     /pseudo
     CDS             complement(4668..4913)
                     /locus_tag="ACHJ8C_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937612.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4975..5133)
                     /locus_tag="ACHJ8C_004395"
                     /pseudo
     CDS             complement(4975..5133)
                     /locus_tag="ACHJ8C_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(5204..>5422)
                     /locus_tag="ACHJ8C_004396"
     CDS             complement(5204..>5422)
                     /locus_tag="ACHJ8C_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5554145.1"
                     /translation="EWVEKHTVQLEFIQPGKPTQNAFIERFNRTYRTEILDFYLFRTL
                     NEVREITEQWLSEYNCERPHESLNNLTP"
BASE COUNT         1441 a         1314 c          945 g         1722 t
ORIGIN      
        1 attatcattt cagtaatatt tatgctggct gcgtggtttt atctggttat ataaagcaca
       61 gatgttctca ttattttctg aaaaataacc atattgatcg atttcccccc cccagccgaa
      121 gcggttcggc tcgttcggcg tgtcgtcgac actgaagcgc ttatccactt cgttccagca
      181 gtaagagtcg tcgctgtcgc tgacgaatgc catcgggtcg ctaaaaagcg tgatccgcca
      241 tgcgatcaaa aaacgtaagt gtttccgaca gacgagccgg tgaaaaaagt ggtgtgtctg
      301 gtaatacagg catcgtcccc gaaatggacg atgccgttga aggactgggg tatggcaata
      361 aaccgcttta ttatcggatt cagtgaccgc ctggacggtt acttctgaga aaaggcattt
      421 acacacaatc ctaaacagac tcctgtattt cagattttgt tatcaacgca tttcaaatct
      481 cttagaaatc attactgtcg tcatattttc cgaggcagct caccacctaa ccatgtctta
      541 tcaacaatat tttgtgaagg atatttataa tgtaaagaca catcagaaac gatattccac
      601 actcaacatg gcagatgtat tatggtatcc atgtactcca agttgaatgc ctgcatcagc
      661 ccggaatgct acttctggtg ttatcattcc cgcaacacca ccatttattt ctccgatatc
      721 acgcgcccca tcctggtata tacttacacc attcatactg gtgccgaaaa cacgactatt
      781 gtgcaaccag tgtgcctcca caaatggtct gaacggaacc tcataccgcg tatttttacc
      841 gtttttgctg gtaagccatg ttcttgctcc caggcgagtg agaatatttc cgttaccggt
      901 attttcaata ctggttccgt tactttcatg atatttgtct gattttacat tcatccggac
      961 aacctgaagc tgcggctgaa cataccattc atgacttccc ttgtaattgt cggtaaactc
     1021 tccagccttc catgaatatc ctgcttccag agataccgta aacccttttg acttccatga
     1081 ctcggcaggc agccgttctc catttatatg attatcaaac cagctgtatt gcagccagct
     1141 atcaagataa gaaccattgt gtgtttcatc attggcatac catgttgcat aaagacccgc
     1201 agtgtatcca ttcattcttc cttccgaatg gtatccggtg ctcagggcat tcgtactgtt
     1261 atgattatga ccatagccag ccatcatacc tgtatgccag cgatcggtac cattcaggct
     1321 ccactgagct atatctcccc ctattaacac aacgtagcta ttcccccggg ttctcagctg
     1381 actactgttg tcacgccagc gattatgttt tcctttatta tacatccaga tacctgcttc
     1441 attttcttct cctggcatgt ggctgatata caactcctgt ccctgtcgtt catacagatt
     1501 cgttacaaac agattatttg cagcagcaat actggcaaca tagctaccag cctcaggacg
     1561 caaattatta tcatggtttt ccggtacagc ttcaggttgt ggtacaggaa tgtcttttct
     1621 gctaatcaga taccagttag tgctgttaat cccctgacca cgtttcagta tgtaatcata
     1681 agctccggct gtaatacgtt ccgtctgaat gaattcagca ttatcagcat tgccactgac
     1741 gtgaatcact tcaatcccat taagtgtctc tgcaccagta ccaccggcct gagtaacagc
     1801 gacacgggta cggccactgg cattaccttt aatcaccaga cgatctgttt tcgaattgtc
     1861 accacctgtt tctgtctgga ggagaataag tccatttctg ccagtgtaat tccctccgac
     1921 agtcagtata ttacccggca tatatgtagt gagatcgact ataccactat tgctcagatt
     1981 tcctgcaacg cccccaaacc cggagagttt gccctgctct tcaacaatga cctggttact
     2041 gccgagcatc accggtgaat ctgcgttacc cagaatcaat gtcccctgaa taatatctgt
     2101 catcccggtc cattttgcgt tatcgctaat gttcagtgca cctgctcccg tcttgcgaac
     2161 attaccataa ccactgatat tgttggtaag ctgccatgct gatgtactgt tcagagttaa
     2221 tgtgccatag ttatcaacaa aggctgtccc cagattatca gccgtggata cagccaactg
     2281 agccccctta ttcacggtaa aaacaccgga aaaataacga ttattacccg ttatatcaag
     2341 cattccccca tttatgttca gagaaccatc ccctgcaata gtacctgcag attctcctcc
     2401 attattcagt atcaggcttc cgccatgaat gttcagcaca gagcctgcag ccgtcagtag
     2461 tttgcttatt gtctgaggat acccattcat atccagttct gcgccacggg ataaatataa
     2521 atactcagtc cgaccaagag catcaggtgc agcaagaatt aatacgccag atttaacctc
     2581 tgtatttcct gtatagttgt ttgttgcaga aagtataaga gtaccattac ctgattttgt
     2641 cagacttttg ccgtcccagc catcatataa taaaggtgaa agattatcgg aaagagaaac
     2701 tcctatgttc agagtggcat ctttttctat attaaaggtt ccatatccca tcccttttgt
     2761 attattccat ctcagcatgg atatcagttt gttatcgtca tcatttaaag acgtataaac
     2821 ataactttca gcaccttctg tcagtaatac attgtattgt acaatgtgac tattatcagc
     2881 gatcgagata cctgtgtctg acgacaataa tgtgtaatag tcctgagtcc agttcttaat
     2941 tgatgtgtct atattaagcc atgaatcact ttttaatgta atagtatttg cgtgaacatt
     3001 agcatttcct gcagacaggt tcatagatgc gtcttcatca atacttatat ttccgttaaa
     3061 aatgatgttc ttgcctgaca gataaacaga cgagccattc ccaatcatta gcccaccatt
     3121 aattgctgtt gcgttattga tgtttttaat aattaattca tttgtaccat taacaggtat
     3181 actagctagt ttaatatcac cattaatcac agaatctgaa taaatatcta atgaattata
     3241 cccaccagaa aaaattattg atgtattata agcatctttt tggctattat atgagcctga
     3301 aatattacca tgattattaa tatggatatc ttctccataa acagagcaac taccgtcggg
     3361 gccatatccc ccctgaacaa taccgttatt attcaaagtg atttgatttc cagaaatagc
     3421 atctccccct tgttttattc catatcctcc agatatcatg ttgtcgttag taatgaaaag
     3481 attactacca ttaataccat caccaccgtt tacgccatta ccaccccata ttcctccgga
     3541 attatgtact gtaatattgt tcccggttac accggaacca cctgtcccac ctagatcatc
     3601 acctccgtat atacttctgt aattatcaac tataatatca tccccgctta accctgcacc
     3661 tccgctactt cctgagccac caagaccatt acccccatat acatttctgt aattgtttat
     3721 agatattttg ttgccactaa cagcaactcc accattatat ccatctcctc cccaaacact
     3781 accattatta tttagcgtaa agttttcacc gtagacacct gcaccaccga ttgatgtatt
     3841 gcttgttcca cctttgatgt taatactgct ttcaacagat agtatatttt gagtataact
     3901 tgttttatat ggcacgctga tattaagcgc cgcgtttcca tcaacacctg taatactcct
     3961 ggttatactt tcaggtatac cattaccatt cagttgtatt gtttgcgcac cagaaaaatg
     4021 agactgcaca gaataaagta tactgaccag aaactgtaaa attcgtatat tcttattcat
     4081 actgtttttt tctttagtga tgcatttttt tatcaaccat acaagcacat tatttcattg
     4141 atactaatca catcaggata aatgtttttt ctcaatagaa ataagtgtct ttattcctta
     4201 ccaatttatt ctggttattc ctcttatcca gacgtctcgt gttactccct gtatttatta
     4261 tagagtatat atatgtactg cgtttaaatc atgaagacga caacctgttt tacataaatt
     4321 attcctaagt attttttcca gagaagacct aggtataatg atgtgcactg tctggttttc
     4381 ttacaactga cttagcgatc tgcatattcg tgataacgcc tgaataaata ggacgagaga
     4441 actcttaacc aaataatcta acacttttaa tccacaggaa ttaaaaaccc ggggaggtta
     4501 aaaaaacaca aaaaaatgga tgtttattca actctggtta cgtaccatga ggactgcatc
     4561 cacaatgcac cggcaattaa atggctttca ttgattgatt ttttatatct gtgatgattt
     4621 tttgctccat tcctcaaaaa atggacgcaa cttcccctct gcggctctca gctgtatacc
     4681 cgctgtcgtg ccagtggctc atgcatcatg accagctctg ccagacggta gccccgcttc
     4741 atccccgtaa accgcatctg acctccgcac aggatgcact tcagcgggtc aaccttcagt
     4801 aaccgctgat acacccttct ccgcctgatt tgcatcgccg tttttctctc cgtttctgtt
     4861 atgacgtaca caacattttc cagtaaccgg cgcttcactg gactcaggaa cccgttgtca
     4921 acgccacgat gtttgatcgt tctttgccat tttcatgccc gctaaatact ttcgttactg
     4981 atgctgtccg ctttgcacca tttcatcatc actgattggc gttgtgtttt tggtcagcac
     5041 tccagcttta cgtttctctt ttaaccggta acgctctcct ttgatattca gcgtggttga
     5101 gtgatgtagc aaccgatcca gtatcgctgt tgctgtaaat agacccgctt tagttccata
     5161 cactttttga gatcccgggc tggtaatgtt gttgacgata ttcctacggt gtcaggttgt
     5221 tcagtgattc atgagggcgt tcacagttat attctgacag ccattgttcc gtgatttcac
     5281 gcacttcatt cagcgttctg aacagataaa aatcgagtat ttctgtgcga tatgttctgt
     5341 taaagcgttc aatgaatgcg ttctgtgtcg gtttacccgg ttggataaat tccagttgta
     5401 ctgtatgttt ctctacccat tc
//
LOCUS       DAZFJL010000199         5389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZFJL010000199 DAZFJL010000000
VERSION     DAZFJL010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.201"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            2..751
                     /gene="paa"
                     /locus_tag="ACHJ8C_004397"
     CDS             2..751
                     /gene="paa"
                     /locus_tag="ACHJ8C_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HFO5554146.1"
                     /translation="MMAGFLIFLSSAAYADINLYGPGGPHTALLEAAKLYAEKTGVIV
                     NVHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSILLVK
                     KGNPKNIRSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRKNII
                     LYAPNSGTARKALENQPGADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITVRQD
                     ANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
     gene            1090..1245
                     /gene="hokD"
                     /locus_tag="ACHJ8C_004398"
     CDS             1090..1245
                     /gene="hokD"
                     /locus_tag="ACHJ8C_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO5554147.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1412..1684
                     /locus_tag="ACHJ8C_004399"
     CDS             1412..1684
                     /locus_tag="ACHJ8C_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554148.1"
                     /translation="MAHNTKNHNSDAVRASRHGRRSRVFKTDWYQHPPCTEEQAEWLI
                     QCYRRHGYEIKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            1686..2750
                     /locus_tag="ACHJ8C_004400"
     CDS             1686..2750
                     /locus_tag="ACHJ8C_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5554149.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVGPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNIALPPHVHQHQQQNPPQEKTVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQ
                     KCMTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQL
                     LLLIRFLMHARNSGVLTWKA"
     gene            2751..3116
                     /locus_tag="ACHJ8C_004401"
     CDS             2751..3116
                     /locus_tag="ACHJ8C_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5554150.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKVGERYRRDVALIVR
                     QQRLKLNLSGRLAIRITAEPPDKRHRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQTVPGGRLGIKITELECA"
     gene            3113..3802
                     /locus_tag="ACHJ8C_004402"
     CDS             3113..3802
                     /locus_tag="ACHJ8C_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO5554151.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPIEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYKELALLVGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKA
                     AFSKQSVAKVD"
     gene            4013..4088
                     /locus_tag="ACHJ8C_004403"
     tRNA            4013..4088
                     /locus_tag="ACHJ8C_004403"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4046..4048,aa:Ile,seq:cat)
     gene            4096..4172
                     /locus_tag="ACHJ8C_004404"
     tRNA            4096..4172
                     /locus_tag="ACHJ8C_004404"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4130..4132,aa:Arg,seq:tcg)
     gene            4186..4262
                     /locus_tag="ACHJ8C_004405"
     tRNA            4186..4262
                     /locus_tag="ACHJ8C_004405"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4220..4222,aa:Arg,seq:tct)
     gene            4436..4864
                     /locus_tag="ACHJ8C_004406"
                     /pseudo
     CDS             4436..4864
                     /locus_tag="ACHJ8C_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310776.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
     gene            4861..5028
                     /locus_tag="ACHJ8C_004407"
     CDS             4861..5028
                     /locus_tag="ACHJ8C_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO5554152.1"
                     /translation="MTVKLRLTVAALLLFLVVMVDFTSRIMSVLADGVLVCGIMVLLW
                     PVIKRNSLHNA"
     gene            complement(5223..5366)
                     /locus_tag="ACHJ8C_004408"
                     /pseudo
     CDS             complement(5223..5366)
                     /locus_tag="ACHJ8C_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1400 a         1264 c         1443 g         1282 t
ORIGIN      
        1 cataatggca ggttttttaa tattcctgtc ttctgctgct tatgctgata tcaatctgta
       61 tggtcctggt ggcccgcata cagccttgct tgaagcagcc aaactttatg ccgaaaaaac
      121 aggtgttata gtgaacgttc attacggtcc acagaacaaa tggaatgaag atgccaaaaa
      181 aaatgcagat atcttgtttg gcgcatcaga acaatctgct ctggctatca ttcgggacca
      241 taaagacagc ttcagtgaaa aagatattca gcctctttat ctgcgaaaaa gtattttact
      301 ggtaaagaaa ggtaatccta aaaatatccg gagtattgac gacctgacca gacccgggat
      361 tggcgtaatt gttaatgatg gtggtggtac cagtaataca tcaggcactg gcgtctggga
      421 agatattgcc ggacgtaaag ggaatataga aactgtcgcc gcaatccgaa aaaatattat
      481 tttatatgcg cccaatagcg gaactgcacg taaggctctt gagaatcagc ctggagcaga
      541 tgtctggata acctgggctg actgggcagc cagtaatcca gaaattggtg atgtcgtgga
      601 aatagcccca gactacgtga tatggcgtga tatgaacatt acagtacgtc aggatgcaaa
      661 tgatgaaacc cgtcgatttg cagaatggct acaaaccgat gaagcggcgc ctgcattcaa
      721 aaaatatggc tggaccagga aaggcacttg acatcctcca cgtccttcag gacgtggatt
      781 ctttttccgg atgccgcgcc agcggcatgt aggggcagct cacaaaacgg aaaaaattgt
      841 acgctaagtc tcgccaggtg aactgaattc attccgatat gggaattccc atatcgggcg
      901 aaaacggttt gctgtaacgg cagagttaag tagaattgct gcgggtgctt gaggctatct
      961 gcctcgggca tgaacaccaa cggcagatag ataaaagccc cacccgacta taaatcgaag
     1021 tgaggcccct atatgctcgt cacatataga ttgcctctta cggaccgaaa ggtcaaggag
     1081 aagcaggcta tgaagcagca aaaggcgatg ttaatcgccc tgatcgtcat ctgtttaacc
     1141 gtcatagtga cggcactggt aacgaggaaa gacctctgcg aggtacgaat ccgaaccggc
     1201 cagacggagg tcgctgtctt cacagcttac gaacctgagg agtaagagac caggcggggg
     1261 agaatccctc gccacctctg atgtgtcagg catcctcaac gcacccgcac ttaacccgct
     1321 tcggcgggtt ttgttttttc ctggcattct ggtttacaat tcgcacgcca gcctgaacaa
     1381 ctggcacctg ctgcgccagc agagaaaaca aatggcgcac aataccaaaa atcacaattc
     1441 tgatgccgtt cgtgccagca ggcatgggcg gcgttctcgc gtatttaaaa ccgactggta
     1501 ccaacaccct ccatgcactg aagaacaggc cgaatggctg attcagtgtt accgcagaca
     1561 cggatacgag attaagaaag ccctcagcct cgattatcgt cactggataa tctccgtcag
     1621 gcttccttac tccgaacgcc caccgcgtcc gtcccgcaca ttccagcaac ggatctggag
     1681 gtaacgtgcg ggtattactt cgacctgttc tggtaccgga actcgggctg gtgatcgtta
     1741 agccgggccg tgaatccatg ccggtattcc acaatacccg ggtattggtg gggccggaac
     1801 cgaaaagcat gcgtaatctg ccgtccgggg tcgttcctgc cgttcgccag ccgctggtgg
     1861 aagacaaaac attgctgccg tttttcagta acgcacgggt gattcgtgct gctggtggtg
     1921 ctggtgcatt gtctgactgg ctgttgcgcc atattaaatc ctgccagtgg ccacacggcg
     1981 attatcatca cagcgaaacc gtcattcacc gttatggtac cggcgcaatg gtgttgtgct
     2041 ggcactgcga caaccagctg cgtgaccaga catccgaatc actcgagcaa cttgctcatc
     2101 aaaacctgtc agcatggatg attgacgtca tcggtcacgc aataagcggt acgcaggagc
     2161 gtgaattatc tttggctgaa ttatcctggt gggcggtccg caatcaggtg gcggacgcgc
     2221 taccggaagc ggtattacgt cgttcgctgg ggttgcgtgc ggaaaaaatc cgctcaatgt
     2281 accgtgaaag cgacatcgta ccgggagagc agaccgccac cagcatactg aagcagcgca
     2341 caaaaaatat tgcgctaccg cctcacgtcc accagcacca gcaacagaac ccaccacagg
     2401 aaaagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat ctccagcgcc
     2461 agaaaccaca acgggaagag atgcctgtat acacgcgttg ggtaaaaacg cagaaatgca
     2521 tgacgtgcgg taatcaggca gatgatccgc accacatcat tggtcatgga ctgggaggga
     2581 tgggaacaaa ggctgatgat ttgtttgtta ttccgctgtg ccgtaaatgc cataacgaac
     2641 tgcacgccgg ggtaaaagat tttgaagaaa aacacggcag ccagctgttg ttgctgattc
     2701 gttttttaat gcacgcgaga aattcgggtg ttctgacgtg gaaagcataa atgactgaac
     2761 gcatagaatt tgttttgcct tacccgccga cggtgaatac ctactggcgc cgccgcggca
     2821 gtacctattt tgtgtcaaaa gtcggtgagc gttatcgccg tgatgtggcg ctaattgttc
     2881 gccagcagcg gctgaaactg aacctgtccg gaaggctggc aatcagaatc accgcagagc
     2941 caccagataa gcgccaccgc gacctggaca atatcctgaa agcaccactg gatgcgctga
     3001 cgcatgccgg actactcata gacgacgagc agtttgatga aatcaatatt gtgcgcggtc
     3061 agactgttcc cggtgggcgg ctggggataa aaatcacaga actggagtgc gcatgaataa
     3121 ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt tgagtggggc
     3181 aacaaaaggt cagcttgaag cctggcagga gaatgccatg ttcgatacag ggcgttacag
     3241 gcgaaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc gggataatcc
     3301 accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg tcagtccgat
     3361 tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagaac atcataaagc
     3421 ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg cgataacaca
     3481 gtgggcgtgg aatgaattta atactcaatc cggtaccaga aaaattgcag ggaaaacgca
     3541 ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag aactttttgg
     3601 tggggaaggt tatgaataca aggagctggc attactggtg ggagtgacaa ctaaaaactg
     3661 gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc gactggatag
     3721 tgaggcttta ttgtttgtaa tgagaacgcg ttcaaaacaa aaggcggcat tttcaaagca
     3781 aagtgttgca aaagtagatt gaaaggcata tatttcatgc aaatctgata ttttgccgat
     3841 tttgtacgtg atggcaaaag caaacaaaac ccgcccacaa gcgggttttt ttgtgccact
     3901 tatctcggat agacatggtg aatgcgctgg tggaggaagc aagggtaatt tttaaccagg
     3961 tgattcttga atgcttgcaa cattgatttc gtaacgttat tatcctgcgc ccggcccttt
     4021 agctcagtgg tgagagcgag cgactcataa tcgccaggcc gctggttcaa atccagcaag
     4081 ggccaccatc acataccgcc attagctcat cgggatagaa cgccagcctt cgaagctggt
     4141 ttcgcggggt tcgagtctcc gatggcggtc cattatcggt attctgcgtt tttagctcag
     4201 ccggacagag caattgcctt ctaagcaatc ggtcactggt tcgaacccag tacaacgcac
     4261 cacacttatt ttccctcgct cgcttttgcg gacctttttt gtatccgcac cacgcccggc
     4321 gcataccaac cacagagcct ttcgggggga gcttatggag tggtcagtgt gactttctct
     4381 gtgggcagat cgctcccggg cgttggctca cccacccaaa ggaacgtcac gatgtttggt
     4441 atttttggta aaaaaagccc gcagagcggc aacggaaatt aaaaagtttg aaaaacgcga
     4501 tctggcacag gtggtgatta acgccgcata cctggtggcc tgtgcagatg gtgaatgtga
     4561 ggcttcctag aaagcgaaga tcgaacaggt actgcgtaat cagcctgcgc tgtacgcgtt
     4621 tacgtcagaa attaatgcga ttagcgcaac cattatcggt cagctggata cgaactttaa
     4681 aactggtcgt cgtgcggcgt tacgtgagat cgaggatgtg aaacacgata cgcgtgaagc
     4741 ggaagatgtg ctggatgtgg cggtggccat tgcggaggca gacggcgaaa ttgagccgga
     4801 agagcgcaag gtgctggaag agattgccgg tgttctgggt cttcgtctgg agaattacct
     4861 gtgacggtaa aactgcgcct gactgtggct gcactcctgc tgtttctggt ggtgatggtg
     4921 gatttcacca gcagaatcat gtcggtgctg gcggatgggg tgctggtctg cggcattatg
     4981 gtattgctgt ggccggtgat aaaaagaaac agcctgcata atgcttgatt tttttgtttg
     5041 ctgtttatta aaaacacttc tgcatggtga atccccctgt gcggtggggc aatcagcaag
     5101 aaggaatatg gggtaatcgc ggattcaggt gctgatactg aattcaccgg gaggcacccg
     5161 gcaccatgct ttgccacaaa agtgttattt ctgtttttct caaactatca tcgttatccc
     5221 tttattttcg gctgcgcatg gcgcggcctt ttttttacga ccagccactg gcagatggtc
     5281 atcctgtgat ttgattccgc ttccggcttt ttaactctgt tcctctacac gggagaaatt
     5341 cgatgtcgat taaacattat gatgttgtca gggcggcgtc gccgtcaga
//
LOCUS       DAZFJL010000200         5301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZFJL010000200 DAZFJL010000000
VERSION     DAZFJL010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.202"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            131..1489
                     /gene="mltD"
                     /locus_tag="ACHJ8C_004409"
     CDS             131..1489
                     /gene="mltD"
                     /locus_tag="ACHJ8C_004409"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO5554153.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1538..2308)
                     /locus_tag="ACHJ8C_004410"
                     /pseudo
     CDS             complement(1538..2308)
                     /locus_tag="ACHJ8C_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(2386..3186)
                     /locus_tag="ACHJ8C_004411"
     CDS             complement(2386..3186)
                     /locus_tag="ACHJ8C_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO5554154.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3427..4341
                     /gene="yafC"
                     /locus_tag="ACHJ8C_004412"
     CDS             3427..4341
                     /gene="yafC"
                     /locus_tag="ACHJ8C_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO5554155.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSGHVKTAPGGAVREA"
     gene            complement(4338..5141)
                     /gene="dkgB"
                     /locus_tag="ACHJ8C_004413"
     CDS             complement(4338..5141)
                     /gene="dkgB"
                     /locus_tag="ACHJ8C_004413"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO5554156.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELDYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPS
                     DEVSAEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGNALKDKVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         1392 a         1267 c         1322 g         1320 t
ORIGIN      
        1 tattgcaaca acctgaagag cgttttgcat ggttaaggtc aaagaaagat aggttctgat
       61 aaaactttct tgtcatcggc tccgttcacc gttatgatcg gtcgtctttt aagcaactat
      121 tgacacacac atgaaggcaa aagcgatatt actcgcctct gtcctgctcg tgggttgcca
      181 gagtaccggc aacgttcaac agcacgcaca gagcctttct gcagctggtc aaggggaagc
      241 agcaaagttt acaagtcagg cacgatggat ggacgatggg acgtctatcg cgccagatgg
      301 tgacttgtgg gctttcattg gcgacgagct aaagatggga attccggaaa acgaccggat
      361 tcgcgaacag aaacagaaat atttacgcaa taagagctat ctccacgatg taactttacg
      421 ggcagagccg tatatgtact ggatagccgg gcaagttaaa aaacgtaaca tgcctatgga
      481 actggtacta ctacccatag tggagagcgc ttttgatcct cacgcaacgt ctggcgccaa
      541 tgccgcgggc atctggcaga tcattccgag cacggggcgc aattatggtt tgaaacagac
      601 acgcaattat gacgcgcgtc gcgatgttgt tgcttcaaca actgccgcgc tgaacatgat
      661 gcagcgtctg aacaagatgt ttgacggcga ctggcttctg accgtagcgg cttataacag
      721 cggcgaaggt cgggtcatga aggcaattaa aacgaacaaa gcgcgtggga aatccacgga
      781 cttctggtcg ttaccgttgc cgcaggaaac gaagcagtac gtgcctaaaa tgctggcatt
      841 gagtgatatt ctcaaaaaca gcaagcgtta tggcgtacgt ctgccaacga ccgatgaaag
      901 ccgtgctctg gcgcgtgtgc acctgagcag cccggttgaa atggcgaagg ttgcagatat
      961 ggcggggatt tccgtcagca agctgaagac attcaacgca ggcgtgaaag gctccacgct
     1021 gggcgcaagt ggtccgcagt acgtgatggt gccaaagaag catgcagatc aactgcgtga
     1081 atctctggct tcaggcgaaa ttgctgctgt acagtcgacg ctggttgccg acaatacgcc
     1141 gcttaacagc cgtgtttaca ccgtacgctc tggcgacacg ctttcaagta tcgcttcacg
     1201 tctcggcgta agcaccaaag atttgcagca gtggaacaaa ctgcgcggat ctaagctgaa
     1261 gccaggccaa agtttgacga ttggtgcagg cagtagcgca cagcgactgg caaacaacag
     1321 cgatagcatt acgtatcgtg tgcgcaaagg cgattcgctt tcaagcattg ctaaacgcca
     1381 cggcgtgaac atcaaagatg taatgcgctg gaacagcgac actgcgaatc tgcaaccagg
     1441 cgataagctg acgttgtttg tgaaaaacaa caacatgcca gattcctgac aaaccagata
     1501 ataaaaaagg caccgattcc cccggtgcct tttttattta tgccgcttta tgtgcttcta
     1561 gcatgatgat atcactggtg aaagagccat cattctgcaa ggcaaaatac gttttcacct
     1621 ctgtcgatgc gctctgttgg taaatgcgaa tagcgtctac taacgcttct ggcgtacgca
     1681 ttctcgctac ccatgaagaa aattccagcg gtaacttatc tgtaattaaa ttatcaacta
     1741 tcaaattggc ttcactgatt aacgtcaacc actcaccgat ggcgtagttc cgtgtgtgag
     1801 atgtatcgcg taatgcttct accgtctgta accagatgtc gcgcactggg tggcccggag
     1861 acattacgtc catcacaatc agcttaccgc caggtttcaa tatccggttc acttcacgca
     1921 gtgctgcacc aacatcatgc cagtgatggg cagaataacg gctgataaca atatcaaatg
     1981 cgttatcggc aaatggcagg ctttcgacat aaccctagcg ggtggtgata tttttcagtt
     2041 gccgggcttc ggcagcttgt gccacgacat ccagcatatg agcagataag tcatacgcca
     2101 ccaccgtgct cacgttttgc gcagcgacaa agctggcatg ccctgctccg cagcccatat
     2161 caagtacact tgcatcagga taatcagcca gacgcaccgc caggcgctgt aaatctcgtc
     2221 cggatgcatg tacggtactg gtcagatatt cgctggcttg agagctaaac tgcttttcta
     2281 cgtggtcatg gtgggattgt gttgtcattg cgctgtcctt ttgttatttg agtataaaag
     2341 ggcagcgcca aacgttggtc tgccctgtgg cagacctgac ataccttatt tatcaggctt
     2401 gccgggactg aattcaacga gtagcggatt gtgatcggac gcgcgcgtaa ccagtacaga
     2461 agcttcgctg acgttcagac cacggtagaa aacaaaatcg agcgggcgac caaacgcccg
     2521 acggcgctga tcatcggtaa aacgcacctg gcgcagcgac atttcccgtg caaagcgata
     2581 taacgcgttc atccttctac ggctccaggc attgaaatct cccgccataa tgaccgggcc
     2641 actgtggtga gctatctgat cgccaatagg aagtaactgc ttactataga catccacgcc
     2701 cagactgaag ttgacggcgt gtatattaac caccatcaac aggcgggtgt caggtaatgg
     2761 atagaccgtc accagtgccg atttcgccag acgcaaaatg ggttctcgtt cacgtaacgg
     2821 gcagcaatac actggatgtg ccgccgaaag ggtcattacg ccagaaggat gttgtggcag
     2881 cacgaaagcg ggtacctgat cggcggcaag atagttagca gtcgcaaact gtactaactc
     2941 tggcgttgtc tgcgcttcct gcaataacac cagatgtgca tctttgccgt agttctttaa
     3001 taccgacaac cattcagcgc gttgctgttt gtatatgttc cacaccagga tccgaatacg
     3061 ctcttcggta cttaacggtt ctccaggtgg taatgcctgg ccgatgctcg caaaagaccc
     3121 cggcggtaag atcctttccg caggttgtcc ggcaacatag cgcatggcat aggtgttttt
     3181 tcgcactttt gactttgaaa cctctatcac gtcaaccctt ccgcactcgg gagggttcta
     3241 ctgttatagg gattttagct cactctttca acgagcaatt gtatattgtt atgtaagcaa
     3301 gtgcttacac cggtcaggat tttgctcatt attgagaata ttaaatcgga tggcatatct
     3361 cgcatcctga tggtttaatg acaaataaat tcaaaatatc tcttctattt tgcaacagga
     3421 gcaaaaatga aagccacgtc ggaagaactc gccatttttg tttcagtcgt cgaaagcggc
     3481 agctttagcc gggcagcgga acaattaggg caagcaaact cagcggtaag ccgggcggtg
     3541 aaaaagctgg agatgaaact tggcgttagc ctgcttaatc ggaccacgcg gcaacttagc
     3601 ctgacggaag aaggcgagcg ttattttcgt cgcgtacagt caattttgca ggagatggca
     3661 gcggcagaat cagaaattat ggagacgcgt aatacaccgc gtggactgtt acggatcgat
     3721 gccgcaactc cagtggtgct gcactttctg atgccgttga ttaagccttt tcgtgaacgc
     3781 tatccggaag tcactttatc gctagtctcc tccgaaacga ttattaattt gatcgaaaga
     3841 aaagtggatg tcgcgatacg cgccggtacg ttaacggatt ccagcttacg tgccaggccg
     3901 ctatttaaca gttatcgaaa aattatcgcc tcccccgatt atatttcccg ctacgggaag
     3961 ccagaaacga tcgacgattt aaagcaacat atttgcctgg gattcactga acccgcttcc
     4021 ctcaatacct ggccgatagc ctgtagcgat ggacaattac atgaggtgaa gtacggtttg
     4081 tcatccaata gtggggaaac actgaaacag ctttgcctga gtggcaacgg gattgcgtgt
     4141 ttgtccgact acatgatcga caaagaaatc gctcgcggag aattggtgga gttaatggca
     4201 gataaagtgt tgccagtaga aatgccattc agtgcagtct attacagcga ccgtgcggta
     4261 agtacgcgca tccgggcttt tatcgatttc cttagcgggc atgtaaaaac agctcccgga
     4321 ggagctgtca gagaggctta atcccattca ggagccagac cttccgggct aaccaggcgg
     4381 tcgttgcaat ccagtgcggc gatcgctttt ttatcttcgg catcaagctg taaattttgt
     4441 gccttaagat tactctccag gttttcacgt ttagtagaag aaggaattac tgagtaacct
     4501 tcccccatag cccacgccag aatcacttga gccggagtcg cattgtgttt agctgcaata
     4561 cgagcaataa ccttatcttt cagtgcattg ccatacgcca gagtcatata ggaagtaata
     4621 tggataccgt gctgtttagc ccaagcgacc actttacggt tttgcagata aggagagagt
     4681 tcaatctggt tagtagcgat gttttcagcg ccaacagcag caatcgcttt ttccatcaat
     4741 gggatcgtga agttggaaat accgatctca cgcgtcagcc cttgtttttt ggcttccagc
     4801 agcgcctgca taaactcttc agcagagact tcatcgcttg gtgacggcca gtggattagc
     4861 gtcagatcaa cataatcggt acgcaatttt tgcaggctct ctttcagact cgggatcaat
     4921 ttgtctttgc tgagattttc aatccagatt ttagtggtga tgtagagttc atgacgtggc
     4981 acgccacttt ctgcaatcgc ctgacctact gcggcttcgt tatcatagat ttgtgcggta
     5041 tcaattgcgc gataatcaag ttcaagcgcc gttttcacag atgaaataac aacgtcgtct
     5101 ttcagacgga aagtgcctaa accaaatgca gggatagcca taatatgcct cttttaatgc
     5161 tattttcgtt aactgaggtg attatgggct tcagactgtt gtggaaaaat ggcgtaaaac
     5221 gcagaggatt tttgcgattt tggcaataat agaaatacaa acgaaaaaaa acctcgagcg
     5281 ttaactcgag gcttcttaat a
//
LOCUS       DAZFJL010000201         5273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZFJL010000201 DAZFJL010000000
VERSION     DAZFJL010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.203"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            44..1258
                     /gene="manD"
                     /locus_tag="ACHJ8C_004414"
     CDS             44..1258
                     /gene="manD"
                     /locus_tag="ACHJ8C_004414"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFO5554157.1"
                     /translation="MKIIAVDVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGRSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            1325..2608
                     /locus_tag="ACHJ8C_004415"
     CDS             1325..2608
                     /locus_tag="ACHJ8C_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5554158.1"
                     /translation="MDVEVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTEEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSTWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            2860..3777
                     /gene="oxyR"
                     /locus_tag="ACHJ8C_004416"
     CDS             2860..3777
                     /gene="oxyR"
                     /locus_tag="ACHJ8C_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO5554159.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3760..5160)
                     /gene="sthA"
                     /locus_tag="ACHJ8C_004417"
     CDS             complement(3760..5160)
                     /gene="sthA"
                     /locus_tag="ACHJ8C_004417"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO5554160.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
BASE COUNT         1186 a         1233 c         1460 g         1394 t
ORIGIN      
        1 attaagtttg aagtgtctat ttacaataca ggagattgca ataatgaaaa ttattgctgt
       61 ggatgttttt gttacttgtc ccgggcgtaa tttcgttacg cttaaaataa ccactgaaag
      121 cggactttgt ggattaggag atgccacgtt aaatggtcgc gaattgtcgg tcgcttctta
      181 tctgaaagat catctttgtc ctcaattaat tggccgcgat gccagccgta tcgaagatat
      241 ctggcaattc ttttataaag gagcttactg gcgacgtggt cccgtcacga tgtcggccat
      301 ttctgccatt gatatggcgt tgtgggatat caaagctaaa gcggcaaata tgccattgta
      361 tcaactgctt ggtggcgctt ctcgtgaagg cgtgatggtt tattgccaca ccaccgggcg
      421 gacgattgat gaagtccttg aagactatgc aaagcaccag cagatgggct ttaaggcgat
      481 tcgtgtgcag tgtggtgtac cgggtatgca aaccacctat ggtctggcga agggcaaagg
      541 actggcgtat gaaccagcaa ccaaaggcct gtggccagaa gagcagttgt ggtcttcaga
      601 aaagtatctc gattttacgc cgaagttatt tgaagcggtg cgtaataagt tcggttttaa
      661 cgaacatctg ttgcatgaca tgcaccatcg cctgaccccc atcgaagcgg cgcgctttgg
      721 gaggagcatt gaggattacc gcctgttctg gatggaagat ccgacgcctg ccgagaacca
      781 ggaatgtttt cgtctgattc gccagcatac cgtgacgccg attgcggtcg gtgaagtgtt
      841 taacagtatc tgggactgca aacagttaat cgaagagcag ctgattgact atattcgcac
      901 gacgattacc catgcaggcg gcattaccgg gatgcgacgg attgccgatt ttgcatcgct
      961 ttatcaggta cgtaccggtt cgcatggccc ttcagattta tcgcctgttt gtcatgcggc
     1021 ggcgctgcat tttgacctct gggtgccaaa ctttggcgtt caggagtaca tgggctattc
     1081 cgaacaaatg ctcgaagtct tcccgcacag ctggcgcttc gatgaaggtt atatgcaccc
     1141 aggcgatgaa ccggggctgg gtatttcttt cgatgagaaa ctggcggcga aatatccgta
     1201 tgacccggcg tatctccccg tcgcacgact ggaagatggc acattgtgga actggtaata
     1261 gcggttcgct taattatttt ctgaatattc atcaaatcga ttaattccgc tcaaggagct
     1321 aaaaatggac gttgaggtgt caaccagtgt tgcgggtaat aaaccgcaac ggattcgtcg
     1381 tattcagaca gtgaccctgg ttttattatt tatggcgggg atcgttaatt ttctcgaccg
     1441 ttcatcattg agcgtggcag gtgaagcgat tcgtggcgag ttaggattat cggcgacaga
     1501 gtttggtgtt ttgctttctg cattttctct gtcttatggt ttttcacaac ttccttccgg
     1561 tattttgcta gaccgctttg gcccacgaat agtgttaggc gcaggtttaa tattctggtc
     1621 attaatgcag gcattaacag gaatggttaa ttcttttagc cactttattt taatgcgtat
     1681 cggtctgggt attggcgaag cgccatttat gcctgcggga gtaaagtcga tcaccgactg
     1741 gtatgcgcaa aaagaacgcg gcacggcgct ggggattttt aactcatcta ccgttatcgg
     1801 tcaggctatc gcgcctcctg ctcttgtatt gatgcaactg gcatggggct ggcggacgat
     1861 gtttgtcatc atcggcgtag cagggattct ggttgggatc tgttggtacg cgtggtatcg
     1921 caaccgggcg cagtttgtcc tgaccgagga agaacggaca tatctctccg cgccggttaa
     1981 accgcgtcca cagttacaat ttagcgagtg gctggcgctg tttaagcatc ggacaacctg
     2041 ggggatgatt ttaggtttct ctggcgtcaa ttataccggt tggctctaca tcgcgtggtt
     2101 acccggttat ttgcaggccg aacaaggttt cagtctggcg aaaaccggtt gggtggcggc
     2161 aattcctttc ctcgcggcgg cggtcgggat gtgggttaac ggtattgttg tcgatcggct
     2221 ggcgaaaaaa ggctatgacc tggcgaagac gcgtaaaacg gcgattgtct gcggtttgat
     2281 gatgtcggca ttaggcacgt tgctggtcgt gcaatcttcc tcgccagccc aggcggtagc
     2341 gtttatctca atggcgctgt tctgcgtgca tttcgcggga acatctacat gggggctggt
     2401 gcaggtgatg gtgtcagaaa caaaagtggc ttccatcgcc gggattcaaa actttggcag
     2461 ttttgttttt gcttcctttg ctccgatcgt aaccggttgg gtcgtggata ccacacactc
     2521 gtttaatctg gcgctggtta tcgcggcctg tgtgacgttc accggggcgc tgtgttactt
     2581 ctttattgtc aaagatcgga ttgagtaacc gttactatca ggctctcttg ctgtgggcct
     2641 gtagaataaa aaaaagcgga tcctggagat ccgcaaaagt tcacgttggc tttagttatt
     2701 cgagttgaga aactctcgaa acgggcagtg acttcaaggg ttaaaagagg tgccgctccg
     2761 tttctgtgag caattatcag tcagaatgct tgatagggat aatcgttcgt tgctatgcta
     2821 cctatcgccg cgaactatcg tggcaatgga ggatggataa tgaatattcg tgatcttgag
     2881 tacctggtgg cattggccga acaccgccat tttcggcgtg cggcagattc ctgccacgtt
     2941 agccagccga cgcttagcgg gcaaattcgt aagctggaag atgagttggg cgtgatgttg
     3001 ctggagcgga ccagccgtaa agtgttgttc acccaggcgg gaatgctgct ggtggatcag
     3061 gcgcgtaccg tactgcgtga ggtgaaagtc cttaaagaga tggcaagcca gcagggcgag
     3121 acgatgtccg ggccgctgca tattggtttg attcccacgg ttggaccgta cctgctaccg
     3181 catattatcc cgatgctgca ccagaccttt ccaaagctgg aaatgtatct gcatgaagca
     3241 cagacccacc agttactggc gcaactggac agcggcaaac tcgattgcgt gatcctcgcg
     3301 ctggtgaaag agagcgaagc attcattgaa gtgccgttgt ttgatgagcc aatgttgctg
     3361 gctatctatg aagaccatcc gtgggcgaac cgcgaatgcg taccgatggc cgatctggca
     3421 ggggaaaaac tgctgatgct ggaagatggt cactgtttgc gcgatcaggc aatgggtttc
     3481 tgttttgaag ccggggcgga tgaagataca cacttccgcg cgaccagcct ggaaactctg
     3541 cgcaacatgg tggcggcagg tagcgggatc actttactgc ctgcgctggc tgtgccgccg
     3601 gaacgcaaac gcgatggggt tgtttatctg ccgtgcatta agccggaacc acgccgcact
     3661 attggcctgg tttatcgtcc tggctcacca ctgcgcagcc gctatgagca gctggcagag
     3721 gccatccgcg caagaatgga tggccatttc gataaagtgt taaaacaggc ggtttaagcc
     3781 atttagcgca gctacccgat aggcttccgc catcgtcggg tagttaaagg tggtgttgac
     3841 gaagtactca atagtgttgc cgccaccttt ctgttccata atcgcctgac cgatatgaat
     3901 aatttcggca gcgcgctcgc caaagcagtg aatacccaga atctcttttg tttcccgatg
     3961 gaacaaaatt ttcagcgtgc ccacgttcat gccgacgatt tgtgcacgtg ccagatgttt
     4021 aaactgggcg cggcccactt catatggcac tttcatcgcg gttagctgct gttcggtttt
     4081 gcccacagag ctgatttccg ggatggtgta aatgccggta gggatatctt caatcagatg
     4141 cgcggtggct tcacctttca ccagcgcctg cgcggcaatg cgcccctggt cataggctgc
     4201 cgacgccagg ctcggataac caatcacgtc gcccaccgcg taaacgtgcg gctgtgcggt
     4261 ctgatacatg ctgttgacct tcagctgtcc gcgactgtca gtttccagcc caatgttctg
     4321 taacgccagc gaatcggtat taccggtgcg accgttggca tagagcagac agtcagcttt
     4381 cagtttttta cccgacttca gatgcatgat cacaccgtcg tcacagcctt cgatcttctc
     4441 gtactcttcg ttgtgacgaa tcactacgcc actgttccag aagtgatagg agagagaatc
     4501 tgacatctct tgatcgagaa atgccagcag gcgatcgcgg gtgttgatca gatccacttt
     4561 tacatccata ccgcggaaga tcgacgcata ttcacagccg atcactccag caccgtaaat
     4621 aagtacatgg cgcggttcgt ggtgcatgct aagaattgag tcgctgtcat aaatgcgtgg
     4681 atgggtgaaa tcaacatctg ttggatgata tggacgagag ccgcaggcaa taacaaattt
     4741 ttcagcagtt agtgtttcga cgctgccgtc cgggcaatcc agcgccaacg tatgctcatc
     4801 aacaaagcga gcgttcccct gcaatatttc acagtgatta cgttcgtaaa atccctgacg
     4861 catgcgcgtt tgttgattaa tcacgttatc ggcatggtta aggatatcgg caaaagaaga
     4921 gcggagcagt cgggaatggt cgctgtatag tgggttttga ttgaattcta taatgcggct
     4981 gacggcgtga cggagagctt tcgacgggat ggtgccccag tgggtgcaac cgccgccaac
     5041 attttgataa cgctcgataa ctgcgacgcg cgcaccttgc ttaaccaggc ccatcgcagc
     5101 gccttcgccg ccggggccgg aacctattac tatggcatcg taatcgtagg aatgtggcat
     5161 ggtagggctt acctgttctt atacataaaa gcaacagaat ggtaacattt tatcgcgggt
     5221 aagccaattg atccccgtca tttatctggc tatatcgtga gcggcctttg ctt
//
LOCUS       DAZFJL010000202         5227 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZFJL010000202 DAZFJL010000000
VERSION     DAZFJL010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.204"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..1412
                     /locus_tag="ACHJ8C_004418"
     CDS             <1..1412
                     /locus_tag="ACHJ8C_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO5554161.1"
                     /translation="VRAINNYGQQGEPATTTFRINAPAKPATIELTPGYFQITAVPRL
                     AVYDPTVQFEFWFSETKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVN
                     LVGKSAFVEASGRASNDAEGYLGLFREKIGKLHLAQGLWELIDNSQLADEMAEMKTTI
                     TETRNEITQTVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKNGIPYVAGIG
                     AGIEDTDGQPLSNILLLADRIAMINPEDGNTTPLFVAQGNQLFMNDVFLKRLFAVSIT
                     SSGNPPTFSLTPEGKLTARNADISGAITANTGTLNNVTINENCVIRGKLSANQIEGDL
                     VKTVGKAFPRNNSYASGTVTVTVYDDQGFDRQIIIPPVLFRGTKHQNFNSPNQQSYWY
                     STCKLQVLKNGVEIFHEPATDVSRVFSSVIDMPAGRGHVTLTFNVSSAGANNWTPTTY
                     ISDLLVVVMKKSTAGISIS"
     gene            1481..2104
                     /locus_tag="ACHJ8C_004419"
     CDS             1481..2104
                     /locus_tag="ACHJ8C_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5554162.1"
                     /translation="MRHLYATILLFTTLLAGIAFPAQAESGHGAFSVGYAQVHPGGVP
                     ALSGTGARADDLKGINVKYRYEFTDHLGGIVALSYASVKKSDTMKTGENTFHYESLRG
                     RYVSLMAGPVWQLSERVSLYGMAGMAYTRWSDSVQDYRRDEVKPGYVKETTTASDGHT
                     ARHLSPAWNAGIQFSPVETVVIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            2169..3482
                     /locus_tag="ACHJ8C_004420"
     CDS             2169..3482
                     /locus_tag="ACHJ8C_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO5554163.1"
                     /translation="MAVKISGVLKDGTGKPIQNCTIQLKARRNSTTVVVNTVASENPD
                     EAGRYSMDVEYGQYRVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMSEDDVRPEA
                     LRRFELMVEEVARHAEEAKKNAGEAETSARNAGISASQAEESAANADTSAGEASESAR
                     QAAESAASAKQSEDASSSSASAAAQKASESSQSAAEAELSRKTAESAAGNAARDATTA
                     TEKARESAESAQSAEQSRIAAEEAVNRIPTVVGPPGPKGEQGPAGPQGPKGDKGERGD
                     TGPVGATGERGPAGDAGPAGPQGPKGDRGERGETGLTGNAGPQGPKGDTGAAGPAGPQ
                     GPKGETGAAGPVGATGPQGPKGDPGETQIRFRLGPASIIETNSNGWFPGTDGALITGL
                     TFLDPKDATQVQGLFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
     gene            3484..3753
                     /locus_tag="ACHJ8C_004421"
     CDS             3484..3753
                     /locus_tag="ACHJ8C_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5554164.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            3755..3874
                     /locus_tag="ACHJ8C_004422"
                     /pseudo
     CDS             3755..3874
                     /locus_tag="ACHJ8C_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4070..4219
                     /locus_tag="ACHJ8C_004423"
                     /pseudo
     CDS             4070..4219
                     /locus_tag="ACHJ8C_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309257.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4440..4634
                     /locus_tag="ACHJ8C_004424"
                     /pseudo
     CDS             4440..4634
                     /locus_tag="ACHJ8C_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687042.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            4646..>5227
                     /locus_tag="ACHJ8C_004425"
     CDS             4646..>5227
                     /locus_tag="ACHJ8C_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO5554165.1"
                     /translation="MPFTFYLSSENQLSIQNIEALQNAARANDTGNIIIGDRQFSVRY
                     QSAMDAFIVNPVQGELYSGLNNTVLDDVISLADVIESRLNGGSSFLDVFDRYMIQTMQ
                     TMMNGNESEPESLATRLSSSAFSVSPEDISCIPEALQCPITLAIPERGVFLRNSKGSS
                     VCSLYDENALSRIINDGGHHPLSREPITASMIVK"
BASE COUNT         1352 a         1133 c         1581 g         1161 t
ORIGIN      
        1 cagtcagggc aattaacaat tatggccagc agggcgaacc ggccaccacc accttccgga
       61 ttaacgcgcc agcaaaaccc gccaccattg aactgacgcc ggggtatttt cagataacgg
      121 cggtcccgcg tcttgcggtg tatgacccga cggtacagtt tgagttctgg ttttcggaga
      181 caaaaatcgc agacacatct caggtggaaa cctctgcccg ttatctgggg accggcagtc
      241 agtggagtgt atccggcccg cacattaagc ccgggaagga tttctggttt tacgtgcgca
      301 gcgtcaacct ggtggggaaa tctgcgtttg tggaagccag tggccgggcc agcaatgatg
      361 cagaagggta tctggggctg tttcgggaaa aaataggaaa actgcatctg gctcaggggc
      421 tgtgggagct gatagacaac agccagcttg cggatgagat ggcggagatg aagaccacca
      481 tcaccgaaac ccgcaatgaa atcacacaga cggtcagtaa aacgctggaa gaccagagcg
      541 ccaccattca gcagatacag cgcgtgcaga aggacacaaa tgatgacctt gctgcacttt
      601 acatgctgaa ggtacagaaa acaaaaaatg gcatacccta tgttgccggt attggagcgg
      661 ggattgagga tactgatggc cagcccctga gcaacatact gctgctggct gaccgtattg
      721 cgatgattaa cccggaggac ggcaacacca cgccgttatt tgtggcgcag gggaatcagt
      781 tgttcatgaa cgatgtgttc ctgaagcggc tgtttgcggt gagtatcacc tcgtccggca
      841 atcccccgac gttttccctg acgccggaag ggaagctgac agccaggaac gcggatatca
      901 gcggagcaat taccgcgaat accggcacgc tcaataatgt caccattaac gagaactgtg
      961 tcatcagagg gaaactgtct gcaaaccaga ttgaaggcga cctggtgaag acggtgggga
     1021 aagcctttcc ccggaataac agttatgcca gcgggacggt aaccgtcaca gtttacgatg
     1081 accagggctt cgaccggcag attatcattc ccccggtgct gtttcgcggg acgaaacacc
     1141 agaatttcaa cagcccgaat cagcagtcgt actggtattc cacctgtaag ctgcaggtgc
     1201 tgaagaacgg ggttgagatt ttccatgaac cggcaacgga tgtcagccgg gtgttctcat
     1261 cggtgataga tatgccggca gggcggggtc atgtcaccct gacgtttaat gtgtcgtcgg
     1321 ccggtgcgaa caactggacg ccgacaacgt acatcagtga tttactggtt gtggtcatga
     1381 aaaaatccac ggcagggatc agtatcagct gacggtttat taacccggac gggcacctca
     1441 ggaggtgcct tttttattga ctgaaaacaa agaggtaatc atgcggcatt tatacgcaac
     1501 gatattattg tttactaccc tgctggcagg aattgccttt cctgcacagg ctgaaagcgg
     1561 acacggtgca ttttccgtgg gatatgctca ggttcacccg ggcggcgtac cggcattgtc
     1621 cggtaccggt gcgcgtgcag atgatttaaa agggattaat gtgaaatacc gttatgagtt
     1681 cacggatcac ctgggcggca ttgtcgcgct gagttatgca tcggtgaaga aaagtgacac
     1741 gatgaagacg ggtgaaaata ccttccatta tgaaagcctg cgcggtcgtt atgtcagtct
     1801 gatggccggc cctgtctggc agctcagtga gcgggtcagt ctctatggca tggccgggat
     1861 ggcgtacacc cgctggtctg acagtgttca ggattaccgg cgtgatgaag tgaaaccggg
     1921 gtatgtgaag gagaccacca ccgccagtga tggtcatact gcgcgtcatc tgtcgccggc
     1981 ctggaatgcc gggattcagt tcagtcccgt agagacggtg gttattgacc ttgcttatga
     2041 aggttccggc agtggcgact ggcgcactga cggtttcatc gtgggtgtcg gctataaatt
     2101 ctgattagcc aggtaacaca gtgttatgac agcccgccgg ttcaggcggg cttttttgtg
     2161 gggtgaatat ggcagtaaag atttcaggtg tgctgaaaga cggcacagga aaaccaatac
     2221 agaactgcac cattcaactg aaagccagac gtaacagcac cacggtggtg gtgaacacgg
     2281 tggcctctga aaatccggat gaggcagggc gttacagcat ggacgtcgag tacggtcagt
     2341 acagagtcat tctgttggtg gaaggattcc cgccgtcaca tgccgggacc atcaccgtgt
     2401 atgaagattc tcaacccggt acgctgaatg attttctcgg tgccatgtcg gaggatgacg
     2461 tccggccgga ggcactgcgc cgttttgaac tgatggtgga agaggtggcg cgtcacgctg
     2521 aggaggcgaa gaagaatgcc ggagaggcgg agacgtcagc gaggaatgcc ggcatatcag
     2581 ccagtcaggc agaagagagc gctgcaaatg ctgacacttc agcaggggag gcatcggagt
     2641 cagcccggca ggcggcagaa agtgcagcct cagcaaagca gtcagaggat gcgtcctcgt
     2701 cctcggcttc tgcggccgct caaaaagcca gtgagtcatc acaaagtgca gcagaagctg
     2761 aattgtcaag aaagacggca gaaagtgcag ccggtaatgc agccagggat gcaacgaccg
     2821 caacagaaaa agcccgggag tcagcagaaa gcgcacagtc agcggaacaa agcaggatag
     2881 cggcggaaga ggccgtaaac cgaatcccca ccgtggtggg acctcccggg ccaaaggggg
     2941 aacaggggcc cgcgggtcct caggggccga agggtgataa gggagagcgc ggtgacaccg
     3001 gccctgtcgg ggcaaccggt gagcggggac cggcaggtga tgctggtccg gcaggcccgc
     3061 aggggccgaa aggtgacagg ggagagcggg gagagaccgg tctgacggga aatgcaggtc
     3121 cacagggtcc aaagggagat accggtgcgg caggcccggc aggcccacag ggaccgaaag
     3181 gagaaacagg tgcggctggc ccggtggggg caaccggacc tcagggacca aagggcgacc
     3241 cgggggagac acaaatccgt tttcgtctgg ggccggcgag cattattgag acaaacagca
     3301 atggctggtt cccgggtaca gatggtgcgc tcatcaccgg actgaccttt cttgacccca
     3361 aagatgccac acaggttcag gggctgtttc agcatttgca ggtcaggttt ggtgacgggc
     3421 cgtggcagga tgttaagggg ctggatgaag tgggcagtga tacaggcaga acaggagaat
     3481 gacatgaata tactaaaaaa acttatgcag cgtctgtgtg gttgcggaaa gcatgatggc
     3541 cgtgaacacg tgcagtcgct tacagcacaa ctgcgactgg ggccggcaga catcctggag
     3601 tcagatgaga atggtattat cccggagcag gacagggtaa tcacgcaggt ggtgatactg
     3661 gatgcggata aaaagcagat acagtgtgtg gtaagaccgc tgcaaatcct gcgtgctgac
     3721 gggacgtggg aaaatattgg cgggatgaag taacccgaca gcttcacaaa accggagtcc
     3781 ggctccggtt tttgttgtca tgtatggggg atgtttgtta ttagagtgtg aagtaataaa
     3841 catgttaata cgatggagtg aaggatgcct ttaaggagca gtgatatgtc catcttgttc
     3901 cctggagggg gaatcataaa ttctgattgc gaccgaggca tggaacacta ctgtagttca
     3961 gggaagttcg gcgatgaagc tcattcttgc gacgcgtaat tattatctgg aatatggttt
     4021 gcgtttgtta ctgaaagaat gccgtgtaat tctggctcaa gaatttttta tgccggagaa
     4081 tcgcagggtt attctggata gtaaagaatc ctggttaata atctgtgata gtcagttggg
     4141 ccatttaatg cgcagcatgt ttcagggacg ccgttttatt cagctggatc tggaagcgtt
     4201 gaaaggggga catgatatac ataatgctgt gcgtaacaga ttgtggacct ggaacaaaaa
     4261 agcacgggca ctgacgatgt cagagatggt ggtgatgttt ggatatatct accgtcagtt
     4321 gcgtccgtcc catctcgcca gtgagatgag ggtaaacata aaaacagtta acactttcct
     4381 gtattcaggg ctggcgaaaa atgggctcaa aagaagtagt gtaaggttgc tggcaatttc
     4441 tgagaacaag cgaatagggc attattctgg agcaggacag ggtaatcacg caggtggtga
     4501 tactggatgc ggataaaaag ctgatacagt gcgtggtgag gccgctgcaa attcggtgtg
     4561 ctgatgggac gagggaaaaa tttgtggatg acatttgttg tggaccttta agtctggagt
     4621 tcaaattaaa atagggagtt ttgttatgcc atttacattt tatttgtcgt ccgagaatca
     4681 attgtcaata caaaatattg aagctttgca aaacgcagcc agagcgaatg atacagggaa
     4741 tattataata ggagacaggc agttttctgt tcgttatcag agcgctatgg acgcttttat
     4801 tgttaatcct gttcaggggg aactgtattc aggtcttaat aatactgttt tggatgatgt
     4861 tatcagtctg gctgacgtta ttgagagtcg gttgaatgga ggaagctcat ttcttgatgt
     4921 attcgacaga tatatgatac aaacaatgca aaccatgatg aatggtaatg agagcgagcc
     4981 tgaatcgtta gcgactcgat tatcttcaag tgctttttcg gtttctcctg aggatatatc
     5041 ctgtataccg gaggcattgc agtgtccgat tacattggca attccagagc gtggtgtttt
     5101 tcttagaaac tccaagggtt cctcagtgtg tagtttatat gatgaaaatg ctctctctcg
     5161 tataattaat gatggggggc atcatccatt aagtagggag ccaataacag catcaatgat
     5221 tgtaaag
//
LOCUS       DAZFJL010000203         5145 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFJL010000203 DAZFJL010000000
VERSION     DAZFJL010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.205"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..1784
                     /locus_tag="ACHJ8C_004426"
     CDS             <1..1784
                     /locus_tag="ACHJ8C_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HFO5554166.1"
                     /translation="RKTLVAGTVAITLGLSASGVMAAGFKPAPPAGQLGAVIVDPYGN
                     APLTALVDLDSHVISDVKVTVHGKGEKGVEISYPVGQESLKTYDGVPIFGLYQKFANK
                     VTVEWKENGKVMKDDYVVHTSAIVNNYMDNRSISDLQQTKVIKVAPGFEDRLYLVNTH
                     TFTAQGSDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLDQDTFY
                     DGRDRDINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFADATHES
                     IETPNGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDPKRDAL
                     LGALDAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAKDDSII
                     LSSRHQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENGLCENSD
                     FDFTYTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGTVQQVW
                     EYGKERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTVGKLNEIDYKTKEVK
                     VEIDVLSDKPNQTHYRALLVRPQQIFK"
     gene            1802..2470
                     /locus_tag="ACHJ8C_004427"
     CDS             1802..2470
                     /locus_tag="ACHJ8C_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFO5554167.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQA
                     DFEAALKEPAVQETLKKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRE
                     LASK"
     gene            2485..3156
                     /gene="dsbI"
                     /locus_tag="ACHJ8C_004428"
     CDS             2485..3156
                     /gene="dsbI"
                     /locus_tag="ACHJ8C_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HFO5554168.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRLLWLLMAVAMGALIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIIRG"
     gene            complement(3202..3990)
                     /gene="ygiD"
                     /locus_tag="ACHJ8C_004429"
     CDS             complement(3202..3990)
                     /gene="ygiD"
                     /locus_tag="ACHJ8C_004429"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO5554169.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPKAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMHPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            4133..4906
                     /gene="zupT"
                     /locus_tag="ACHJ8C_004430"
     CDS             4133..4906
                     /gene="zupT"
                     /locus_tag="ACHJ8C_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO5554170.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(4968..5138)
                     /locus_tag="ACHJ8C_004431"
     CDS             complement(4968..5138)
                     /locus_tag="ACHJ8C_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554171.1"
                     /translation="MFIAWYWIVLIVLLAGGYFLHLKRYCKAFRHDRDALLAARSKLD
                     KRSDKEVFEKHQ"
BASE COUNT         1325 a         1264 c         1263 g         1293 t
ORIGIN      
        1 atagaaaaac actcgtagcc ggaactgtgg cgataaccct gggtttgtca gcatcggggg
       61 tgatggctgc gggttttaaa ccagcgccac ctgccgggca actgggtgcg gtcattgtcg
      121 atccctacgg caatgcacca ctgaccgctt tggttgactt agatagccat gttatttctg
      181 acgtcaaagt taccgtccat gggaagggcg aaaaaggcgt agaaatcagc tatcccgtgg
      241 gtcaggaatc actaaaaact tacgatggtg taccgatttt tggtctttat cagaaatttg
      301 ctaacaaagt gaccgttgag tggaaagaaa acggcaaggt catgaaagat gattatgtgg
      361 tgcacacttc ggccatcgtc aataattaca tggataaccg ctctatctcc gatttacaac
      421 agaccaaagt tattaaagtc gcaccgggtt ttgaagatcg cctctatctg gttaataccc
      481 acacctttac cgcccaaggt tccgatctcc actggcatgg tgagaaagat aaaaatgccg
      541 gtatccttga tgcgggtccg gcaactggcg cacttccttt tgatatcgcg ccattcacct
      601 ttatcgtcga taccgaaggt gaataccgct ggtggctgga tcaagacact ttctacgatg
      661 gtcgtgaccg cgatatcaac aagcgtggtt atctgatggg tatccgcgaa acgccacgcg
      721 gcacctttac cgctgtacaa ggtcagcact ggtacgagtt cgacatgatg gggcaggtgc
      781 tcgaagatca caaactaccg cgcggatttg ctgacgccac tcatgagtcc attgagacgc
      841 caaatggcac ggtactgttg cgcgtaggta agagtaacta tcgtcgcgat gacggcgtac
      901 acgtcaccac cattcgtgac catatcctcg aagtcgataa atctggtcgc gttgtcgatg
      961 tatgggatct gaccaagatc ctcgatccga aacgcgatgc actgctcggc gcgctggatg
     1021 caggtgcagt ttgcgttaac gttgaccttg cccatgcagg acaacaggca aaactggaac
     1081 cagatacacc gttcggcgac gcgctgggtg tagggccagg ccgcaactgg gcgcacgtga
     1141 actctatcgc ctatgacgca aaagatgact ctatcatcct ctcctctcgc caccagggtg
     1201 ttgtgaaaat tggtcgtgat aagcaagtga aatggatcct tgcgccctct aaaggttggg
     1261 aaaaaccgct ggccagcaag ctgctgaaac cggttgatgc taacggtaag ccaattacct
     1321 gtaacgaaaa tggcctgtgc gaaaactcag acttcgactt tacctacacc cagcataccg
     1381 cctggatttc cagcaaagga acgctcacca tttttgataa tggcgatggg cgtcatctgg
     1441 aacaacctgc cttaccaacc atgaaatact ctcgctttgt ggaatataag attgatgaga
     1501 agaaaggcac cgttcagcaa gtgtgggaat acggtaaaga acgtggctac gatttctata
     1561 gcccgattac ctccatcatt gaatatcaag ccgaccgtaa caccatgttt ggcttcggtg
     1621 gttctattca tttgttcgat gtcgggcagc caaccgtcgg taagttgaac gaaatcgatt
     1681 acaaaaccaa agaagtgaaa gtggaaatcg acgtgctgtc agataaaccc aatcagactc
     1741 actaccgtgc gctgttagtc cgtccacaac agattttcaa ataattaaga agggaataaa
     1801 aatgtcgaaa ttagggatct catcactatt taaaaccata ctgcttaccg cagctttggc
     1861 tgtttcattt accgcttctg catttaccga aggcacggat tacatggttc tggaaaaacc
     1921 tattccgaat gccgacaaaa cgctgattaa ggtatttagc tacgcctgcc cgttctgtta
     1981 caagtacgac aaagcggtta ccggtccggt atcggaaaaa gtgaaagata tcgttgcttt
     2041 cacgccgttc catctggaaa ccaaaggtga atacggcaaa caagcaagcg aagtctttgc
     2101 ggtcttgatc aataaagata aagcagcagg catttcctta tttgatgcta actctcagtt
     2161 taagaaagcc aagtttgcct actacgcggc ttatcacgac aaaaaagaac gctggtctga
     2221 cggtaaagat ccggctgctt ttatcaaaac cggtctggat gccgcaggta tgagtcaggc
     2281 ggattttgaa gcggcactga aagaaccagc ggttcaagaa acactgaaaa aatggaaagc
     2341 ctcttatgat gtcgccaaaa tccagggggt tccagcctat gtcgttaatg gtaagtactt
     2401 gatctacacc aagagcatca aatccatcga cgcgatggca gaccttatcc gtgagttagc
     2461 aagcaaataa cagggaaaca ggctatgggt attaagggaa tgtggaaaga tcttcgcaca
     2521 tcaccagttg acacactggt cagatggcag gagcaacgct tgttgtggtt gttaatggcc
     2581 gttgcgatgg gcgcgttaat tatcctggcg cactctttct tccagatcta tctctacatg
     2641 gcaccctgtg agcaatgtgt atacattcgc tacgccatgt ttgtgatggt tattggagga
     2701 ttggttgcag cgatcaaccc aaagaacatc atcctaaagc taattggctg cgtgatggca
     2761 ttttacggca gcattttggg actgaagttt tcgctcaaac tgaacgatat ccaccatgca
     2821 gtacataatc cggatcccga ttcactgttt ggcgtacagg gttgctctac cgatcccact
     2881 tttcccttta acctgccgct ggcacagtgg gcaccaaatt ggttcaagcc taccggggat
     2941 tgtggctatg acgcaccaat cgttcctgat ggcgtcacac tgagcagcac gcaacagtgg
     3001 tttgtggaaa tgtatcagca atctgaaggc tggtatctgc taccaccatg gcattttatg
     3061 aatatggctc aggcttgtat gctggctttt ggtatgtgtc tggtgctgct ggtcattatg
     3121 agtggcgcat gggcgcttaa aatcatcaga ggctaactca gtaacacccg aagccagtcg
     3181 ttctggcttc gggttctttt attaacctat ctgcaccgac agcatactta aactgcccat
     3241 ttctatacca tcgaccggaa tggtaattgg ctcctgccca tcccacgccc ctaagacata
     3301 caacaacggc agatagtgct ctggcgttgg gttcgataac gcgccacctt catggtcgag
     3361 ataattcacc agaggatgct gctctaccgg cccttgccac gtcagattcg ctttcacata
     3421 ctcattaaac gacatcgccc aaggatacgg tgaactatca ccgtgccact tcaccgtacg
     3481 caggttatgc accacgttac cgctggcgac caacattatt ccttcatccc gcagcgctgc
     3541 cagtttgcgc cccatttcga aatgccaggc ggcaggtttg ctactgtcga tactcaactg
     3601 caccatcggg atatcggcgt caggatgcat cttaatcagt acgccccacg agccgtggtc
     3661 gaagccccag gcttctttat ccagcgtaac agggaccggc gctaacagct caaccagatg
     3721 ctgtgccagc gcaggcgaac ccggagcagg ataatgcgta tcgtacagtg cctgcgggaa
     3781 gccaccaaaa tcatgaatcg tgggcggcgt ctccatcgcg gtcactcctg ttccccgggt
     3841 aaaccagtga gccgaaacca ccacaatcgc tttcgggcgt ggcaatgtca ttcccaacgt
     3901 ctgccagctg cgggtataca aattatcttc cagcacgttc atcggactac cgtgacctaa
     3961 aaacaatgct ggcatacgtg ttgaagacat gatgatatcc ttaactaaag gtgtcatttt
     4021 gatatcctca caatacgctt gttcggcgga gtaagaaccc ggataacaat gatgatgatc
     4081 atcagttatt ttgacgatct gcctgaaggt gaagatttat aaggagttgt cgatgtcagt
     4141 acctctcatt ctgaccatac tggcgggggc agccacgttt attggcgcgt ttctcggcgt
     4201 tctcgggcaa aaaccctcga accgcttact ggcgttttcg ctaggatttg cggcggggat
     4261 catgttgctc atctcattaa tggaaatgct tcctgccgca ctagccgctg aaggaatgtc
     4321 gcctgtgttg ggttatggaa tgtttatctt cggtctgctt ggctattttg gcctggaccg
     4381 catgttgcca catgcgcatc cgcaggattt aatgcaaaaa tcggtgcagc cgttgccaaa
     4441 atcgatcaag cgcacagcca ttctgctcac tctcggcatc agtctgcata acttcccgga
     4501 agggattgcc acctttgtca cggcgagcag caacctggag ctgggatttg gcatcgcctt
     4561 ggccgtagcg ttgcacaata ttcctgaagg tctggccgtt gcagggccgg tttacgctgc
     4621 aacaggatcg aaacgtaccg cgattctgtg ggcggggatt tccggactgg ctgaaattct
     4681 cggtggcgtt ctggcgtggt taattcttgg cagcatgatt tctccggtag tcatggcggc
     4741 aatcatggcg gcagttgccg gtattatggt ggcgctctcc gttgacgaat taatgccgtt
     4801 agccaaagag atcgacccca ataataaccc cagttatggc gtgctatgtg gaatgtcagt
     4861 gatgggattc agtttagtgc tgctacaaac ggcaggaatt ggttaaagac gataatgaca
     4921 gatgttgtcc attgctaaag gcaacatctg tatcgttatt aaaggtatta ttgatgcttt
     4981 tcaaacactt ctttatcgga gcgtttatct aatttacttc gggcagctaa cagcgcatct
     5041 ctgtcatggc gaaaagcttt acagtaacgt ttaagatgaa gaaaatagcc accagccagt
     5101 agaacaatta atacaatcca gtaccaggca ataaacatcc ttttc
//
LOCUS       DAZFJL010000204         5071 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZFJL010000204 DAZFJL010000000
VERSION     DAZFJL010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.206"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(163..2481)
                     /gene="yicI"
                     /locus_tag="ACHJ8C_004432"
     CDS             complement(163..2481)
                     /gene="yicI"
                     /locus_tag="ACHJ8C_004432"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO5554172.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYVTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTSMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDSTLLALGNNDQRPDYAW
                     HEGTAFHLFNLQDGHEAVCEVPTADGSVIFTLKAARTGNTIIVTGTGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(2491..3873)
                     /gene="yicJ"
                     /locus_tag="ACHJ8C_004433"
     CDS             complement(2491..3873)
                     /gene="yicJ"
                     /locus_tag="ACHJ8C_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO5554173.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTATSMR
                     DDLRDIWQNDQWRIVGFLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            4166..4260
                     /locus_tag="ACHJ8C_004434"
     tRNA            4166..4260
                     /locus_tag="ACHJ8C_004434"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4200..4202,aa:Sec,seq:tca)
     gene            4569..4928
                     /locus_tag="ACHJ8C_004435"
                     /pseudo
     CDS             4569..4928
                     /locus_tag="ACHJ8C_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1392 a         1318 c         1148 g         1213 t
ORIGIN      
        1 aagcaaggcg gttaagggat atttttcagc acagatcaca ttttcgtgat gaataatcgt
       61 gatcatgctc gcaaatacga tggaagaaat atttattacc agatgtgaga ttatttaccg
      121 gatacgacac aaaaccattc gtatccggca ttcttcaata gattacaacg taattgtcag
      181 cgcattccct tgaggagtca ccaccagtcc ctgctcactt tcagcctgcg aaccgccttg
      241 cagaccattt actttcacaa cattgcgcaa gcacagtgtc cagttcttcg cctcgcccgt
      301 accagtcaca ataatcgtgt tgccagtacg tgctgctttt aaagtaaaga tcaccgaacc
      361 gtcagcagtg ggcacttcac agacagcctc atgcccgtct tgcagattaa agaggtggaa
      421 tgccgtacct tcgtgccacg cgtaatcggg acgttgatcg ttgttgccca gcgccagtag
      481 ggtgctatca cgcacataaa cgggcaaact caggaagctg tgctgctgtt tatgccagcg
      541 actaccgtcg agttcatcgt tgtgccacag gtgtgtccag cgaccttccg gcaggtagaa
      601 ctgcacatcg cccgcttcag tgaacacagg cgcaaccatc acgttgtcgc ctaacatgta
      661 ttgacggtca aggtaatcac aagccggatc gtccgggaac tccatcatca tggcccgcat
      721 catcgacgta ccccgcgcgt ttgcacgtgc agcttcacga tacagatacg gcatcatgcg
      781 gcatttcagt tgcgtgaaga agcgcaccac atcacaggac tcatcatcgt aggcccacgg
      841 cacacgataa gatttgctgc catgtaaacg gctatggctg gaaagcaaac caaacgcgca
      901 ccagcgtttg taaacgtgcg ccggagcggt attttcaaag ccgccgatat cgtggctcca
      961 gaagccaaaa cctgaaaggc caatagacaa accaccgcgc aggctttccg ccattgattc
     1021 gtagttagcg taacagtcgc caccccagtg taccgggaat ttctgcgcac cgacggaggc
     1081 cgagcgggca aacaagacgg cttcttcctc accaacggtg tccttgagca cgttccacac
     1141 cagttcgttg tagatgtacg cataatggtt atgcattttc tgcggatcgg aaccatcaaa
     1201 ccattgaaca tcggttggaa tacgttcgcc aaagtcggtc ttaaagcaat caacgcccat
     1261 cgcgaccaga cctttcagtt tgtcggcgta ccatttgcag gcatccggat tggtaaagtc
     1321 ataaatcgcc agacctggct gccatttatc ccactgccac agcgaaccgt ccgggcgttt
     1381 gagtaaataa cctttctctt gtaactcttt aaagacgggg gatttttgcc caatataggg
     1441 gttaatccag acgcagattt tcagcccttt cgctttcagg cggcggatca tcccttccgg
     1501 gtccggaaaa gtcagcgggt cccactcaaa atcgcaccac tggaaggctt tcatccagaa
     1561 acagtcaaag tggaaaacat gcagcggcag attgcgttcc gccataccat cgataaagct
     1621 gtttaccgtc gcttcgtcgt agttggtggt aaatgaagtg gttagccaca gaccgaagga
     1681 ccacgcgggc ggcagcgccg gacggccagt aaaacgggta taacgatcaa gtaccgcttt
     1741 cggcgtcggg ccgtcgataa caaagtattc gagatattca ctctcaacgc tgaactgtac
     1801 tttggagact ttctccgatc ccacttcaaa agagacgcat tggggatgat tgaccagtac
     1861 cccataacca cggttagtca cataaaacgg gatattttta tacgcctgtt cagtacttgt
     1921 gccgccgtcc cggttccagg tctctaccgt ctgaccattg cgcaccaggg cagtaaagcg
     1981 ctctcccaga ccgtaaactg tttcgccaac gccaagatcc agccgctcaa acatataatt
     2041 tcgttgatta ttcgtgtcct gcacgtagcc attatttttc acctgactac cggtaatacg
     2101 ttcgccgttg cgcagaaaat ccagtgacca gaactcacct ttgctgacac gcgcacttaa
     2161 gttgccactt ttaaactcag cgtaacgttc tgtgttttcg atagtgacct tcacgtcctg
     2221 caaaatattg agcgggtaat gaggaccgtt attcagcgcc ccctgaaaat gctcaatccg
     2281 tacaccgaca ataccttcct gtggggagaa aaagcgcagc gtaaataaag gagtatcaag
     2341 ctgccaggta cgttcacgca catcacgagg ggcagcatag accaccattt cattaccctg
     2401 ctgttcaacc tcgaacacct gaagcgggtg aatcaaattg aggccaggtt gaatcaacca
     2461 gtttccgtcg ctaattttca ttgccgttcc ttagttctgc acttcttgag aggtgaattg
     2521 ttgctggcaa cggcgtttgc cctgcgccag ctgttccata acggttttca gattgtgcgt
     2581 cgtgagcgag tagtagcgtt tagcgataat cgcgctcagc aagtaacaga tcgccggaac
     2641 aatcgtgaat agcgcgataa tgatgctaat tgtggcgctg ttctgcgctt tttctgccgc
     2701 atcatatccg ccataagcca gcatccagcc gataagggca ccaccgaagg ccaaccccag
     2761 tttgagcaca aacagcgtgc cagcaaaact gatcccggtc aggcgtttac cattacacca
     2821 ctcgccgtag tcgacggtat cggacatcat tacccactgg ataggtgtca ccagttgatg
     2881 caacacacca atcacgaaga tgaagacaaa catggtgatg ctggcctgca taggaacaaa
     2941 gaacatcgcg aggctaatca ctgccagcag ggcgttcgtc caccagaaga tagtgacttt
     3001 acatttccag tcggtcagcg gtttcgccag tgcggaacca atcaggttac ccacgcaata
     3061 agtggtgaga aaagcaacaa acacttccgg cgtacccaaa atccatgtga cgtaatacat
     3121 catcgcccca ccgcgtacgc acaccgccag gatattgaaa attgtcagga aaccgacaat
     3181 acgccactgg tcgttttgcc agatatcacg taaatcatcc cgcatagacg tcgctgtagg
     3241 tggtgcttca acgcgttctt tagtggtgaa gaaacagaat gccagcatca taaatgccac
     3301 cacggaaagg accgcgatac cgccctggaa accgagtggt ttattatcac caccaattaa
     3361 attaaccagt ggcatcatca gaacagtaga gagcatgcct cccgcggtcg ccagcacaaa
     3421 acgccaggat tgcagcgaga tacgctgagt cgggtcattg gtgattacac cacccaatgc
     3481 gcagtaaggg atattgacga tggtataaag taaggtaagt agggtgtaag taattgctgc
     3541 ataaatcatt tttccgttca tactgagatc tggcgtgcta taggccagta cacagacgat
     3601 cccgaatggc agtgcgccaa acagcaccca cggccgaaat ttaccccagc gagagcgcgt
     3661 tcgatcggcc aacaacccca tgcaaggatc ggaaatcgca tccagtgcac gagcgaccaa
     3721 aaacatggtt ccgacaaatc cggcaggaat gccaaaaata tcggtataaa agaacatcat
     3781 atataacatt acgttatcga aaataatgtg gctggcggcg tctcccatgc cataaccaat
     3841 tttctcttta acggacaaca cttcactctt catctgcttt tccttgatgc tataggggtg
     3901 ctgagaccga taacgcattt tgtaaaccat ccctgcgaga gcgaatattc cgatatctgg
     3961 ttattaattt atgattcttg ttttatgtga tcgtggtagc gttaatttcg ctcatatatc
     4021 attgtaaaat atgattttta tatgaactat aatgctttcg tgataatacg ccgcgtgtat
     4081 taggcggaaa aaactgatct gggggatgta gaaactcaag gaagtagcta taatgcgccc
     4141 cgcctccatg tagcaatcga ggcgcggaag atcgtcgtct ccggtgaggc ggctggactt
     4201 caaatccagt tggggccgcc agcggtcccg ggcaggttcg actcctgtga tcttccgcca
     4261 aaatgcctct tctgaactct cccgacgtta acaaaacccc ttctaatccc ttatatatca
     4321 ggctttcttt gtgtccagag atcaatcgac tcaacctgta tcaagttgct tttggggata
     4381 ctttaggggg tattatttcc tcttgaaata ttagcctccc ataataggaa aaaggtaata
     4441 cataacaatg gtttttaaca gcatctttgt tatacaaggg ggcattgagg atatcaggaa
     4501 aaatctcaaa atcggtgcgg acaggaaact acgtgggcag caaaaacgta tggtaacgcc
     4561 gggaaatgca acgcccacca gtcgggctaa tccaccactc caatcgtggt tcgcagtact
     4621 gcacacacga ttaccgggct atacaggagc ggtttggtct gaaaacatcc atgccgcgta
     4681 aaggtaaccg ttacgacaac acctcaatga aaaacttata gtaaacgctg aagaatgaaa
     4741 ctatcgtttt aataaccggg atgaagccat ctaagtaata cggaaataca ttgagatttt
     4801 ctacaaccat cagcgccgcc actcccgtct ggggaatatc tccctggtag ccttcaggaa
     4861 aaatataacc agataactgc gcaaaaaaaa gaacaaatgg tggtgtccgc tattgccagt
     4921 atacctgagt ttactgcctt tcagattatc acaatcaata gcgaacagac ttttgagctc
     4981 tgtgcgcttt taacaaaacg tctctttctc cactttcacc gtataataat acattattta
     5041 ttacccctcc aataaaaacc gatattacta a
//
LOCUS       DAZFJL010000205         5049 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFJL010000205 DAZFJL010000000
VERSION     DAZFJL010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.207"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..685
                     /locus_tag="ACHJ8C_004436"
     CDS             <1..685
                     /locus_tag="ACHJ8C_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HFO5554174.1"
                     /translation="GLLNAWSLAGEHRHWMISLRKGAQYEEIRKLGKGDHLVKLKTSP
                     QARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGY
                     REIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFS
                     ESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPT
                     APKKSQSVA"
     gene            946..1287
                     /gene="raiA"
                     /locus_tag="ACHJ8C_004437"
     CDS             946..1287
                     /gene="raiA"
                     /locus_tag="ACHJ8C_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO5554175.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    1390..1515
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            1391..1438
                     /gene="pheL"
                     /locus_tag="ACHJ8C_004438"
     CDS             1391..1438
                     /gene="pheL"
                     /locus_tag="ACHJ8C_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO5554176.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            1537..2697
                     /gene="pheA"
                     /locus_tag="ACHJ8C_004439"
     CDS             1537..2697
                     /gene="pheA"
                     /locus_tag="ACHJ8C_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO5554177.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPQWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGDITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2740..3861)
                     /gene="tyrA"
                     /locus_tag="ACHJ8C_004440"
     CDS             complement(2740..3861)
                     /gene="tyrA"
                     /locus_tag="ACHJ8C_004440"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO5554178.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAAEIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRH"
     gene            complement(3872..4942)
                     /gene="aroF"
                     /locus_tag="ACHJ8C_004441"
     CDS             complement(3872..4942)
                     /gene="aroF"
                     /locus_tag="ACHJ8C_004441"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO5554179.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
BASE COUNT         1373 a         1284 c         1211 g         1181 t
ORIGIN      
        1 gggactgtta aatgcctgga gcctggcggg agaacaccgc cactggatga tatctctcag
       61 aaagggagcg caatatgaag agatcagaaa actgggtaaa ggcgatcatc tggtgaagct
      121 gaaaaccagc ccgcaggcac gaaaaaagtg gccgggactg ggaaatgaag tgacagcccg
      181 cctgctgacc gtgacgcgca aaggaaaagt ctgccatctg ctgacgtcga tgacggacgc
      241 catgcgcttc cccggaggag aaatggcgga tctgtacagt catcgctggg aaatcgaact
      301 gggatacagg gagataaaac agacgatgca actgagcagg ctgacgctga gaagtaaaaa
      361 gccggagctt gtggagcaag agctgtgggg tgtcttactg gcttataatc tggtgagata
      421 tcagatgatt aaaatggcgg aacatctgaa aggttactgg ccgaatcaac tgagtttctc
      481 agaatcatgc ggaatggtga tgagaatgct gatgacattg cagggcgctt caccgggacg
      541 tataccggag ctgatgcgcg atcttgcaag tatgggacaa cttgtgaaat taccgacaag
      601 aagggaaagg gccttcccga gagtggtaaa ggagaggccc tggaaatacc ccacagcccc
      661 gaaaaagagc cagtcagttg cttaactgat cggcattaca gcccttgagc tgccgttttt
      721 ttattctgtt agttgtgaaa ctgaagcgat ttagtcacta tcgatctcat caaatatggc
      781 tcgctttgag atattcctca agtaaaaaaa cacctcttcc tgcgatttct cacaaaaaag
      841 attcgttgac aaaaagtgac aaaattatga gatttccatc acacattttg acatcaggaa
      901 cggtatgctg aattcaccaa gacgggaaga caagaggtaa aatttatgac aatgaacatt
      961 accagcaaac aaatggaaat tactccggcc atccgccaac atgtcgcaga ccgtctcgcc
     1021 aaactggaaa aatggcaaac acatctgatt aatccacata tcattctgtc caaagagcca
     1081 caagggtttg ttgctgacgc cacaatcaat acacctaacg gcgttctggt tgccagtggt
     1141 aaacatgaag atatgtacac cgcaattaac gaattgatca acaagctgga acggcagctc
     1201 aataaactgc agcacaaagg cgaagcacgt cgtgccgcaa catcggtgaa agacgccaac
     1261 ttcgtcgaag aagttgaaga agagtagtcc tttatattga gtgtatcgcc aacgcgcctt
     1321 cgggcgcgtt ttttgttgac agcgtgaaaa cagtacgggt actgtactaa agtcacttaa
     1381 ggaaacaaac atgaaacaca caccgttttt cttcgcattc ttttttacct tcccctgaat
     1441 gggaggcgtt tcgtcgtgtg aaacagaatg cgaagacgaa caataaggcc tcccaaatcg
     1501 gggggccttt tttattgata acaaaaaggc aacactatga catcggaaaa cccgttactg
     1561 gcgctgcgag agaaaatcag cgcgctggat gaaaaattat tagcattact ggcagagcga
     1621 cgcgaactgg ccgtcgaggt gggaaaagcc aaactgctct cgcatcgccc ggtacgtgat
     1681 attgatcgtg aacgcgattt gctggaaaga ttaattacgc tcggtaaagc gcaccatctg
     1741 gacgcccatt acattactcg cctgttccag ctcatcattg aagattccgt attaactcag
     1801 caggctttgc tccagcaaca tctcaataaa attaatccgc actcagcacg catcgctttt
     1861 ctcggcccca aaggttctta ttcccatctt gcggcgcgcc agtatgctgc ccgtcacttt
     1921 gagcaattca ttgaaagtgg ctgcgccaaa tttgccgata tttttaatca ggtggaaacc
     1981 ggccaggccg actatgccgt cgtaccgatt gaaaatacca gctccggtgc cataaatgac
     2041 gtttacgatc tgctgcaaca taccagtttg tcgattgttg gcgagatgac gttaactatc
     2101 gaccattgtt tgttggtctc cggcactact gatttatcca ccatcaatac ggtctacagc
     2161 catccgcagc cattccagca atgcagcaaa ttccttaatc gttatccgca atggaagatt
     2221 gaatataccg aaagtacgtc tgcggcaatg gaaaaggttg cacaggcaaa atcaccgcat
     2281 gttgctgcgt tgggaagcga agctggcggc actttgtacg gtttgcaggt actggagcgt
     2341 attgaagcga atcagcgaca aaacttcacc cgatttgtgg tgttggcacg taaagccatt
     2401 aacgtgtctg accaggttcc agcgaaaacg acgttgttaa tggcgaccgg gcaacaagct
     2461 ggtgcgctgg ttgaagcgtt gctggtactg cgcaaccaca atctgattat gacccgtctg
     2521 gagtcacgcc cgattcacgg taatccatgg gaagagatgt tttatctgga tattcaggcc
     2581 aatcttgaat cagcggaaat gcaaaaagca ttgaaagagt taggggacat cacccgttcg
     2641 atgaaggtat tgggctgtta cccaagtgag aacgtagtgc ctgttgatcc aacctgatga
     2701 aaaggtgccg gatgatgtga atcacccggc actggattat tagtgacggt tgtcattcgc
     2761 ctgacgcaat aacacgcggc tttcactctg aaaacgctgt gcgtaatcgc cgaaccagtg
     2821 ctccaccttg cggaaactgt caataaacgc ctgcttatcg ccctgctcca gcaactcaat
     2881 cgcctcgccg aaacgcttat agtaacgttt gattaacgcc agattacgct ctgacgacat
     2941 aataatgtcg gcatacagtt gcggatcctg ggcaaacagt cgcccaacca tcgccagctc
     3001 aaggcggtaa atcggcgaag aaagcgccag aagttgctca agctgaacat tttcttctgc
     3061 caggtgcagc ccataagcaa aagtagcaaa gtggcgcagt gcctgaataa acgccatatt
     3121 ctgatcgtgc tcgacagcgc taatacgatg cagccgagcg ccccagacct gaatttgctc
     3181 cagaaaccat tggtatgctt ccggcttacg tccatcacac cagaccacaa cttgttttgc
     3241 caggctaccg ctgtccgggc cgaacatcgg gtgtaacccc agcaccggac catcatgcgc
     3301 cgccagcatg gcctgtaatg gtccattttt caccgatgcc agatcgacca gaatacaatc
     3361 tttcggtaaa ggcggtaatt tgccaataac ttgttcagta acgtggatgg gcacactaac
     3421 aatcaccatt ccggcatcgg caactatctc agccgctcga tcccagtcat gttgctccag
     3481 aatccgcacc tgataacccg atagtgtcag catcttctcg aacaggcgtc ccatctgacc
     3541 gccaccgccg acgataacca ccgggcgcag tgacggacaa agcgttttaa atcctttgtc
     3601 gttttcactg gagtaagatt cacgcatcac ccgacgcaaa acatcctcaa tcagatctgg
     3661 cggtacaccc agagcttccg cctctgcacg acgcgaggcc aacatagatg cctcgcgctc
     3721 cggaacataa ataggcagtc caaagcggct tttcacctcg cccacttcag caaccagttc
     3781 cagacgcttc gctaataaat tcagcagcgc tttatcgact tcatcaattt gatcgcgtaa
     3841 tgcggtcaat tcagcaacca taataaacct cttaagccac gcgagccgtc agctgcccgt
     3901 tcagatcctg atgaatttca cgcagcaagg catcggtcat ttcccagcta atgcaggcat
     3961 cggttacgga tacaccgtat ttcatttcac tgcgcggttg ctcggaagac tgattgccct
     4021 cgtggatatt actttcgatc atcagaccaa taattgagcg attgccatct ttgatttgag
     4081 caaccacgga ttctgccacc gcaggctgac ggcgataatc tttattggaa ttaccgtggc
     4141 tgcaatctac catcagagac gggcgcagtc ccgcctgttc catctctttt tcacattgcg
     4201 caacatccgc agggctatag ttcggcgctt taccaccgcg caggatcaca tgcccgtccg
     4261 gattcccctg agtttgtagc aacgccacct gccctgcctg gttaatgcca acaaaacggt
     4321 gcggctgggc ggcggcacgc atagcgttaa tagccgttgc aaggctgccg tcggtgccgt
     4381 ttttaaaacc aaccggcatg gaaagcccgg aggccatttc acggtgtgtt tgcgattccg
     4441 ttgtacgagc accaattgct gaccagctaa acagatcgcc caggtattgc gggctattag
     4501 gatctaacgc ttccgtcgcc agtggcagtc ccatattcac cagctcaagc agcaatttac
     4561 gcgcgatctg cagcccggct tctacatcaa aagagccatc catatgggga tcgttaatca
     4621 accctttcca accgacagtg gtacggggtt tttcaaaata gacgcgcatt accagataga
     4681 ggctatcgct gacctctgcg gcaagggctt taaatcgacg agcatattcc agagcagttt
     4741 ccggatcatg aatggaacaa ggaccacaga ctaccaacag acgaggatcg cgcccggcga
     4801 taatatctga aatggtttta cgcgagtcag caatctgggc ttcctgttgc aggcttaatg
     4861 gaaaagcggc cttcagttgt tccggagtca ttaaaacttg ttcgtcggta atatgtacgt
     4921 tattcagcgc gtctttttgc atgatggcga tcctgtttat gctcgtttgc gatagttgat
     4981 cctcagcgag gatgacgtaa cgataacaca taaagtaaag ttttcaatcc atatttcgta
     5041 catttttct
//
LOCUS       DAZFJL010000206         4923 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZFJL010000206 DAZFJL010000000
VERSION     DAZFJL010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.208"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            133..1467
                     /gene="garP"
                     /locus_tag="ACHJ8C_004442"
     CDS             133..1467
                     /gene="garP"
                     /locus_tag="ACHJ8C_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO5554180.1"
                     /translation="MMLDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGMGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1483..2253
                     /gene="garL"
                     /locus_tag="ACHJ8C_004443"
     CDS             1483..2253
                     /gene="garL"
                     /locus_tag="ACHJ8C_004443"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO5554181.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2283..3173
                     /gene="garR"
                     /locus_tag="ACHJ8C_004444"
     CDS             2283..3173
                     /gene="garR"
                     /locus_tag="ACHJ8C_004444"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO5554182.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETAATAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKTIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3270..4415
                     /gene="garK"
                     /locus_tag="ACHJ8C_004445"
     CDS             3270..4415
                     /gene="garK"
                     /locus_tag="ACHJ8C_004445"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO5554183.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDSGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4448..4824
                     /gene="rnpB"
                     /locus_tag="ACHJ8C_004446"
     ncRNA           4448..4824
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHJ8C_004446"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1152 a         1182 c         1408 g         1181 t
ORIGIN      
        1 gcacaatgat tattattctc gcgctgctta tactaattcc cgagttcatt gtcgtaacgg
       61 cataaatgaa actcataaat aacaccttcc ccataataat aaaaatcagc ataagtaccc
      121 gaggtaaata aaatgatgct ggacaccgtt gacgaaaaaa agaaaggcgt gcatacccgc
      181 tatttaatat tactgattat ttttattgtt accgccgtta actacgccga tcgtgcaacg
      241 ctgtctattg ctggtaccga agtggcaaaa gagttgcagt taagtgcggt ttcgatgggt
      301 tacatcttct ctgcttttgg ctgggcctac ttgctgatgc aaattcccgg cggctggctg
      361 cttgataagt ttggctcgaa aaaagtttac acctacagcc tctttttctg gtcgctgttc
      421 accttcctgc aaggctttgt tgatatgttc ccgctggcct gggcagggat ctccatgttc
      481 tttatgcgat ttatgctggg gttctcggaa gcgccatcat tcccggcgaa cgcccgtatt
      541 gtcgccgcct ggtttccgac aaaagaacgt ggtactgcct ccgccatctt taactcggcg
      601 caatatttct cgctggcgct cttttcgccg ctgcttggct ggctgacttt cgcctggggc
      661 tgggagcacg tcttcaccgt tatggggggg atgggttttg tgctgacggc gctgtggatc
      721 aagttgattc ataacccgac agatcaccca cgtatgtctg cggaagagct gaagtttatc
      781 tcggaaaatg gcgcggtggt cgatatggac cacaaaaagc cgggcagcgc ggcagcaagc
      841 ggtcccaaac tgcattacat caagcaactg ctctctaacc gcatgatgct gggcgtattt
      901 ttcgggcaat attttatcaa caccattacc tggttcttcc tcacctggtt cccaatttat
      961 ctggtgcagg aaaaaggcat gtcgattctg aaagtgggtc tggtcgcctc gattccggca
     1021 ctgtgtggtt ttgcgggcgg cgtgctggga ggtgtcttct cggattatct tatcaaacgc
     1081 ggtttatccc tgaccctggc acgtaagcta ccgattgtgc tgggaatgtt gctggcttcc
     1141 accatcatct tatgtaacta caccaacaac accacgctgg tggtcatgct gatggcgctg
     1201 gctttctttg gcaaaggatt tggtgcgctg ggctggccgg taatttctga caccgcgccg
     1261 aaagagattg ttggcctctg cggcggcgtc tttaacgtct ttggcaatgt tgcctccatt
     1321 gtcactccac tggtgattgg ctatctggta agtgaactgc actccttcaa tgcagcactg
     1381 gttttcgtgg gatgttcagc gctgatggcg atggtctgct acctcttcgt agttggcgac
     1441 attaaacgta tggaattgca gaaataagca aaggtataag cgatgaataa cgatgttttc
     1501 ccgaataaat tcaaagccgc attggctgcg aaacaggtac aaattggttg ctggtcagca
     1561 ctctctaacc cgattagcac tgaagttctt ggtttggctg ggtttgactg gctggtgctg
     1621 gatggcgaac atgcgccaaa cgatatctct acgtttattc cgcagttaat ggccctgaaa
     1681 ggcagcgcca gcgcgccagt agtacgagtg ccgaccaacg agccggtaat tattaagcgt
     1741 cttctggata tcggtttcta taacttcctg attccttttg tagaaacaaa agaggaagca
     1801 gagcaggcgg tggcatcaac ccgttaccca ccggaaggca ttcgcggcgt ctccgtttct
     1861 caccgcgcca atatgtttgg caccgtggcg gattatttcg ctcagtcgaa caagaacatc
     1921 actattctgg tccagataga aagtcagcag ggcgtagata acgtcgatgc tattgccgct
     1981 accgaaggtg tagacggcat cttcgtcggc cccagcgatc tggctgcggc attaggccat
     2041 ctcggcaatg cctcgcaccc ggatgtacaa aaagcaattc agcacatttt taaccgtgcc
     2101 agcgcgcacg gcaaacccag cggcatcctc gcaccagtcg aggccgatgc gcgtcgttat
     2161 ctggaatggg gcgcgacgtt tgtggccgtc ggcagcgatc tcggcgtctt ccgctctgcc
     2221 actcagaaac tggctgatac ctttaaaaaa taaccaccac cgcaataaga gagatgattg
     2281 atatgactat gaaagttggt tttattggcc ttgggattat gggtaaacca atgagtaaaa
     2341 accttttgaa agcaggttac tcgctggtgg ttgctgaccg taacccagaa gctatcgctg
     2401 acgtgattgc tgccggtgca gaaacagccg ctacggctaa agcgatcgct gaacagtgcg
     2461 acgtcatcat caccatgctg ccaaactccc ctcatgtgaa agaggtggcg ctgggtgaga
     2521 atggcattat tgaaggcgcg aagccaggta cggtattgat cgatatgagt tctatcgcac
     2581 cgctggcaag ccgtgaaatc agcgaagcgc tgaaagcgaa aggcattgat atgctggatg
     2641 ctccggtgag cggcggtgaa ccgaaagcca tcgacggtac gctgtcagtg atggtgggcg
     2701 gcgacaagac tattttcgac aaatactatg atttgatgaa agcgatggcg ggttccgtgg
     2761 tgcataccgg ggaaatcggt gcaggtaacg tcaccaaact ggcaaatcag gtcattgtgg
     2821 cgctgaatat tgctgcgatg tcagaagcgt taacgctggc aaccaaagcg ggcgttaacc
     2881 cggatctggt gtatcaggca attcgcggtg ggctggcggg cagtaccgtg ctggatgcca
     2941 aagcgccgat ggtgatggac cgcaacttca aaccgggctt ccgtattgat ctgcatatta
     3001 aggatctggc gaatgcgctg gatacttctc acggcgtcgg cgcgcaactg ccgctcacag
     3061 ctgcggttat ggagatgatg caggcactgc gtgcagatgg tttaggaacg gcggatcata
     3121 gcgccctggc gtgctactac gaaaaactgg cgaaagtcga agttactcgc taatgacgtc
     3181 gtgcccggat ggcgtattgc aatccgggcc tggaatccag gccgaataag agaaacgccc
     3241 tccggcgtca tgtggtaaca ggcataggta tgaaaatcgt aatcgcccca gactcttata
     3301 aagaaagttt atctgccagc gaggttgcgc aggcgataga aaaaggattt cgggaaattt
     3361 ttcctgatgc acagtatgtt tctgttccgg ttgccgacgg tggtgaagga acggtggaag
     3421 cgatgattgc agccacccaa ggggctgaac gtcacgcctg ggttacaggg ccgctgggcg
     3481 agaaagtgaa tgccagttgg gggatctccg gcgatggcaa aaccgcgttt atcgaaatgg
     3541 cggcggccag tgggctggaa ctggtacctg cggaaaaacg cgatccactc gtgaccactt
     3601 cacgcggtac tggcgagtta atcctgcagg cgctggagag cggtgcgaca aacattatta
     3661 tcggcattgg cggcagcgcc accaacgacg gcggcgcagg catggtacag gcgctggggg
     3721 cgaaattatg cgatgccaac ggcaatgaaa ttggttttgg cggcggtagt cttaatactc
     3781 tgaatgatat tgatatttcc ggtctcgatc cgcgcttaaa agattgtgtc atccgcgtcg
     3841 cttgtgatgt caccaacccg ttagtcggcg acagtggtgc ttcgcgcatc tttggcccac
     3901 aaaagggagc cagtgaggcg atgattgtcg agctggataa taacctctct cactatgccg
     3961 atgtcattaa aaaagcgctg catgttgatg tgaaagatgt ccccggcgca ggagctgcgg
     4021 gcggtatggg cgcggcgctg atggcgtttc ttggtgcaga actgaaaagt ggtattgaaa
     4081 tcgtcactac ggcgctgaat ctggaggaac atattcacga ttgtacgctg gtgatcactg
     4141 gtgaagggcg tattgatagc cagagtattc acggcaaggt gccgattggt gtcgcaaacg
     4201 tggcgaagaa gtaccataaa ccggtgattg gcattgcggg tagcctgacc aatgatgttg
     4261 gcgttgtaca tcagcatggc attgatgcgg tcttcagcgt actgaccagc attggtacgt
     4321 tagacgaagc attccgcgga gcttatgaca atatctgccg tgcttcacgt aatatcgccg
     4381 cgacactggc gattggaatg cgcaacgcgg ggtgacaagg gcgcgcaaac cctctatact
     4441 gcgcgccgaa gctgaccaga cagtcgccgc ttcgtcgtcg tcctcttcgg gggagacggg
     4501 cggaggggag gaaagtccgg gctccatagg gcagggtgcc aggtaacgcc tgggggggaa
     4561 acccacgacc agtgcaacag agagcaaacc gccgatggcc cgcgtaagcg ggatcaggta
     4621 agggtgaaag ggtgcggtaa gagcgcaccg cgcggctggt aacagtccgt ggcacggtaa
     4681 actccacccg gagcaaggcc aaataggggt tcataaggta cggcccgtac tgaacccggg
     4741 taggctgctt gagccagtga gcgattgctg gcctagatga atgactgtcc acgacagaac
     4801 ccggcttatc ggtcagtttc acctgattta cgtaaaaacc cgcttcggcg ggtttttgct
     4861 tttggtgccc agggtaagat gaatatctac ggtattaata acagactttg taagaagtta
     4921 tta
//
LOCUS       DAZFJL010000207         4909 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFJL010000207 DAZFJL010000000
VERSION     DAZFJL010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.209"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..81
                     /locus_tag="ACHJ8C_004447"
     CDS             <1..81
                     /locus_tag="ACHJ8C_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5554184.1"
                     /translation="NTGGNKEQVPAKRQNRGSRNSTDDGG"
     gene            107..646
                     /locus_tag="ACHJ8C_004448"
     CDS             107..646
                     /locus_tag="ACHJ8C_004448"
                     /inference="COORDINATES: protein motif:HMM:NF010292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5554185.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGAQTQPEEGQQFSGQPQPEPQAEAGTKKGGAKTKGRGRKAAQPE
                     PQPQPPEGDDYGFSDDIPF"
     gene            708..941
                     /locus_tag="ACHJ8C_004449"
     CDS             708..941
                     /locus_tag="ACHJ8C_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO5554186.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAVQYRNVFIEDDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            1006..2963
                     /locus_tag="ACHJ8C_004450"
                     /pseudo
     CDS             1006..2963
                     /locus_tag="ACHJ8C_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
     gene            3018..3452
                     /gene="psiB"
                     /locus_tag="ACHJ8C_004451"
     CDS             3018..3452
                     /gene="psiB"
                     /locus_tag="ACHJ8C_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO5554187.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDTQGYSVNDIIHILMAEGGQV"
     gene            3449..4211
                     /locus_tag="ACHJ8C_004452"
                     /pseudo
     CDS             3449..4211
                     /locus_tag="ACHJ8C_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            complement(4180..4368)
                     /locus_tag="ACHJ8C_004453"
     CDS             complement(4180..4368)
                     /locus_tag="ACHJ8C_004453"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554188.1"
                     /translation="MKINLLRGYLLSATQHGNKPPSRHEAESLKRRAHHSPYTCTLTT
                     LTFPENNPLIQTVHGKSV"
     gene            4390..4539
                     /gene="mok"
                     /locus_tag="ACHJ8C_004454"
                     /pseudo
     CDS             4390..4539
                     /gene="mok"
                     /locus_tag="ACHJ8C_004454"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            4481..4606
                     /locus_tag="ACHJ8C_004455"
     CDS             4481..4606
                     /locus_tag="ACHJ8C_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFO5554189.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK"
BASE COUNT         1100 a         1277 c         1541 g          991 t
ORIGIN      
        1 aataccggag ggaacaaaga gcaggtcccc gcaaaacgtc aaaaccgcgg ctcccgaaac
       61 tcaacagatg atgggggctg aaaacatcaa cagaaggaga cacatcatgg cagttcgtgg
      121 aattaacaag gtcattcttg tcggtcgtct gggaaaagat ccggaagtcc gttacatccc
      181 caacgggggc gcagtggcaa acctgcaggt ggccacgtca gaaagctggc gtgacaaaca
      241 gacgggggag atgcgggagc agacagaatg gcatcgcgtg gtgctgttcg gcaagctcgc
      301 ggaagtggca ggtgaatatc tgcgcaaggg cgcgcaggtc tacatcgagg ggcaactccg
      361 aacccgtagc tgggaagata acggtatcac ccgttacgtc accgaaattc ttgttaagac
      421 cacgggcacc atgcagatgc tgggacgtgc cgcaggtgct cagactcagc cggaagaggg
      481 gcaacagttc agcggtcagc ctcagccgga accacaggcg gaggccggta cgaaaaaagg
      541 tggcgcaaaa acgaaaggcc gtggacgtaa ggccgcgcag ccggagcctc agccgcaacc
      601 gccggagggt gacgattacg ggttttcaga cgatatcccg ttctgaacga ctgactgtga
      661 caacggcccc gccccgtcct gtacggggca tcaccggaga gatgaggatg agcgaatatt
      721 tcagaatact tcaggggctg cctgacggct cctttacccg cgaacaggcc gaagccgttg
      781 ccgtacagta ccggaacgtc tttatcgagg atgatcacgg cgaacagttt cgcctggttg
      841 tccgtaataa cggtgcaatg gtctggcgta cctggaattt tgaggacggt gccgggtact
      901 ggatgaacca tgtcatccgt gatttcggga ttcttaagta agagaggtgc cggacgggga
      961 tcacgtccgg cagacataca accaccccgg tcaaaggaga aatatatgtc agttacagag
     1021 tctaaggtaa aaacggagcg taaatccagc cgtaaacctg caaaaacgca ggaagcagtc
     1081 ctgtcggccc tgctggcgga gacggctgaa gtgagcgtgc cgctggcctc gctgattaag
     1141 tcaccgctga atgtgcgcac ggtgccgtat tccgcagagt ccgtcagcga actggcagag
     1201 tccattaagg gcgtcggcct gctgcagaat ctggttgttc atgccctgcc aggtgaccgt
     1261 tacggtgtcg ccgcaggcgg tcgccgactg gcagcactca acatgctggc agagcgcaac
     1321 atccttccgg ctgactggcc tgtccgcgtg aaggttattc cgcaggaact ggcgactgct
     1381 gcatcaatga ctgaaaacgg tcatcgtcgg gatatgcacc ctgccgaaca gattgccgga
     1441 ttccgtgcaa tggcgcagga aggcaaaacg cctgcacaaa tcggtgattt gctgggctat
     1501 tcaccccgcc acgttcagcg aatgctgaaa ctggcagacc ttgcgcctgt catcctcgat
     1561 gcgctggcag aagaccgcat caccacagag cactgtcagg cgctggcgct ggagaacgac
     1621 accgcgcgtc aggtgcaggt gtttgaagct gcctgtcagt cgggatgggg cggtaaaccg
     1681 gaagtacaga ccattcgtcg tctggtgacc gaaagtgaag tggcggtggc ggggaacact
     1741 aaattccgct ttgtgggggc tgatgccttc tcgccagatg aactgcgcac cgatttgttc
     1801 agcgacgacg agggtggcta tgtggactgt gttgcgctcg atgctgccct gctggaaaaa
     1861 ctccaggctg ttgctgaaca ccttcgggaa gccgagggct ggggatggtg tgccggacgc
     1921 atggaggccg ttggtgagtg ccgtgaagat gcccgggcat accgcaacct gccggagccg
     1981 gaagcggtgc tgacggaggc agaagaagaa cgcctgaacg aactgatggc acgttacgac
     2041 gcgctggaaa accagtgtga ggaatccgac ctgctggaag cagaaatgaa gctgattgac
     2101 tgcatggcga aggtcagggc gtggacgccg gagatgcgtg ccggaagtgg cgtggtggtg
     2161 tcctggcgtt acggtaatgt gtgtatccag cgtggtgtgc agttgcgcag tgaggatgat
     2221 gtggctgacg acgctgaccg cacggaacag gggctggaga gcgcgtcagt ggaggaaatt
     2281 agtctgccgt tgctgactaa attgacctca gagcgcacgc tggcagtcca ggcagcactg
     2341 atgcagcaac cggacaaatc cctggcactg ctgacatgga cgctctgcct gaatgtgttt
     2401 gacagcaaag cgcatagtag tcccgccaga attcgcctgg aatgtgagca ttatgcgctg
     2461 accagcgatg caccgtcagg gaaggaaggt gccgcattca tggcgctgat ggcagaacac
     2521 tctcgttttg ctgccctctg ccggaaggat gggaacggga tatgacgacg ttcctgtcac
     2581 ttagccagga ggtgctgtta tccctgctca gtttctgtac cgcgtgcagt cttaacggtg
     2641 tccagacccg cgagtgtggt cacacgtcac gcagtccgct ggactcgctg gaaaccgcca
     2701 tcggctttca catgcgcgac tggtggcagc cgacaaaagc aaacttcttc ggacacctga
     2761 aaaagccgca gattatcgca gccctgaatg aggccggact gtccggtgcc gcacgggacg
     2821 cggagaaaat gaagaaaggc gatgcggctg aacatgcaga gttccatatg aaagacaacc
     2881 gctgggtgcc tggctggatg tgtgcaccac gtccacagac ggatgccact gaacacaccg
     2941 ctaacctggc tgatgccgcc tgatgaacaa ccacaccgcc ccgccggaga cggggcggca
     3001 gcaagggaga taccgtgatg aaaactgaac tgaccctgaa tgccctgcag tccatgaacg
     3061 cacaggaata tgaagatatc cgtgctgcgg gaagcgatat gcgccgtaat ctcactcacg
     3121 aggtgatgcg tgaagtggac gcaccggcta actggatgat gaacggagag tatggcagtg
     3181 agttcggggg ctttttcccc gtccaggttc gtttcacgcc agcccacgaa cgtttccacc
     3241 tggcattatg ttcaccgggc gatgtctctc aggtctgggt gctggttctg gtgaatgccg
     3301 gtggtgagcc gtttgcagtg gttcaggtac agcggcggtt tgcaccggaa gccgtcagcc
     3361 attcactggc actggcggcg tcactggata cgcagggata cagcgttaac gacatcatcc
     3421 atatcctgat ggcggaagga ggtcaggtat gagcgcacgt tcacaggcac tggttcccct
     3481 cagcacagag caacaggccg catggcgggc ggtggcggag acagaaaaac gccgccatca
     3541 gggaaacttg ctggcggaat atccgtatgc gggggcgttt ttccgctgtc tgaacggaag
     3601 ccgcaggatg tcgttgtctg acctgcgatt tttcatgccg tccctgaccg cagaagaact
     3661 gcgcggcaac cgttctcagt ggctgtatgc catcgatgtg ctgattgaaa cccagggcga
     3721 ggtctgcctc cttccccttc ccggcgatgc tgcagagcgg ttgtttccgt cggtgcggtt
     3781 tcgtgtcagg gagcgcagtc gccataaatc cgcgctggtc atgcagaagt acagccgcca
     3841 gcaggcacgg gaggcagagc agaaagcccg tgcgtaccag gcgctggtcg cccaggctga
     3901 aatcgaactg gcgttccatt caccggaaac agtcggaagc tggcatgcac gctggagtga
     3961 ccgggtggca gagcatgacc tggaaaccct gttctggcag tggggcgagc gttttccgtc
     4021 actggccgga atggaacgct ggcagtggca ggatatgccg ttctggcagg ttattgcgga
     4081 agccagtctt gcagcaagag aggccggtca cgctgtccgt gagatggagc ggtggatggt
     4141 gccgaacaaa ctccgggagg cggcgtgatg cggcaacaat cacacggatt tcccgtgaac
     4201 ggtctgaatg agcggattat tttcagggaa agtgagtgtg gtcagcgtgc aggtatatgg
     4261 gctatgatgt gcccggcgct tgaggctttc tgcctcatga cgtgaaggtg gtttgttgcc
     4321 gtgttgtgtg gcagaaagaa gatagccccg tagtaagtta attttcatta accaccacga
     4381 ggcatcccta tgtctagtcc acatcaggat agcctcttac cgcgctttgc gcaaggagaa
     4441 gaaggccatg aaactaccac gaagttccct tatctggtgt gtgttgatcg tgtgtctcac
     4501 actgttgata ttcacttatc tgacacgaaa atcgctgtgc gagattcgtt acagagacgg
     4561 acacagggag gtggcggctt tcatggctta cgaatccggt aagtagcaac ctggaggcgg
     4621 gcgcaggccc gccttttcag gactgatgct ggtctgacta ctgaagcgcc tttataaagg
     4681 ggctgctggt tcgccggtag cccctttctc cttgctgatg ttgtacgggc atgaacaacc
     4741 tgacttcagg aaggtcgttt cccttcagga acggggatga acgcgcgcct gcggcgcccg
     4801 ttcttttccc ccgccttctc tggttatggc ctgtcagaaa tcacatcacc ttccctgtga
     4861 ttattatctt tttcttcctg ctctgattct gactactgca gtgttgtcc
//
LOCUS       DAZFJL010000208         4387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFJL010000208 DAZFJL010000000
VERSION     DAZFJL010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.210"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(106..1503)
                     /gene="ccp"
                     /locus_tag="ACHJ8C_004456"
     CDS             complement(106..1503)
                     /gene="ccp"
                     /locus_tag="ACHJ8C_004456"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO5554190.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LANKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1908..3557
                     /locus_tag="ACHJ8C_004457"
     CDS             1908..3557
                     /locus_tag="ACHJ8C_004457"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO5554191.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGIRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARNWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3608..4210)
                     /locus_tag="ACHJ8C_004458"
     CDS             complement(3608..4210)
                     /locus_tag="ACHJ8C_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO5554192.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQHRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     ESGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         1132 a         1133 c         1008 g         1114 t
ORIGIN      
        1 aaacgacttt ttataatata aacatttaat atgataatat taaaaacaga ctttattatc
       61 gtaataaaaa tcgcccaaac actcctgttc gggcgatcaa attaattatt gtttatcctg
      121 catatacggc gtgtaaaccc cgttcagact gtgcaggaaa gcgacgatat catccacatc
      181 ctcctgcggc agctctttgc ctacctgata gcgcagcatc agtttcaccg ccccgtccag
      241 cgtcggcacg tcaccgcgat ggaagtacgg tgccgttaaa gcaacgttac gtaaaccggg
      301 tactttctga cgcaatttat cacgctcttc tttagtaaca ttcatacgac caatatccgc
      361 cgccgtaatt tcaccaaagt taaagtcttt tttcagcccc aacggttcaa aggaacgtcc
      421 gccgagaata ataccaccat gacaagttgc acatttatta tctttaaata attgatagcc
      481 ttttttctgt tgcgccgtca gagcattctc atctccacgc aaccatttat caaatgggga
      541 atccggcgta attaatgttt tctcaaattc agcaatggca tcagtaatat tttcgccact
      601 gaaaccttgc ggatagactt cgaggaactg cgctttaagc tgcggatctt tttccagctt
      661 agcaataatt tcgtcccagg atttcgacgc catttcaatc gggttcaacg gcggtccacc
      721 agcctgatcc tgcaatgttg ccgcacgacc atcccagaac tgctcaacgt taaatactga
      781 gttaaatacc gtcggcgcgt taatcggccc cactgcgcca ccaacaccaa tcgatgtttt
      841 tctaccatcg acgcctcccg cattcaacgc atggcaatga gcgcatgaaa tggtgctatc
      901 agccgataaa cgaggatcgt gatacagcgc aaagcccaac gccacttttt gcgcatcggt
      961 aggcagcttt tgcgggatgg gctgcaccgg ttcattgcga tgctccggag cagtatcatt
     1021 gctggcgtaa tattccgcgc gctgttttgc aatccaggcc agtatttccg cccgctcttc
     1081 atcactcacc ttacccgccc agtgtagcgc ggtataacgc gttggcggca tagtttcata
     1141 ctgcatcacc cattcaatct tattcagatc gctttgcgaa acgggtttat tagccagcag
     1201 agccgcacgc acggcctcga ggttaaaaga tttatatcca agctttatgt cgtaatccat
     1261 caactgtttc gcgccgggaa tataataata ggcaggtaat tctgccgaag gcgtatggca
     1321 ataatcgcat cctttttcgc ggagaaagcc taaaacctta ttattttcac tgacagcaga
     1381 tgcctgaaca tcggcctgtt tactgcgttt attatcgtgg taccacacat aaccagataa
     1441 ccctaaatag caaatcgcga cgccagccag gccgatcgcg gtaatacgtg agaccatttt
     1501 cattattctt ccctcacggt ggtgagttat tgtgacccag aaaattttcc agaatgctgc
     1561 aaacgtctct gcgagacaaa gccatcataa aaaacaggga gatatgcttt ttgataggaa
     1621 tcaatcaatt acatgaatat aaactatcaa taagatagcc agtgcctatt acccagatca
     1681 ggaatggcat atcatgattg cggcgagagc aggattgaat gttctgaaaa tgaaaaacaa
     1741 cacgaaatcc gtatttttat aaattccatt gatattagtg cgttatattt tttggcaggt
     1801 taataaacgt gatccaccgc acgctttgtc gcccaccagg cggagcgaat gactaccctt
     1861 aaagaaaagc ccgataatta gcgacgaatt tcggaggtcg gatccttatg ctcaatcaga
     1921 aaattcaaaa ccctaatcca gacgaactga tgatcgaggt cgatctctgc tatgagctgg
     1981 acccgtatga attaaaactg gatgagatga tcgaggcaga accggaaccc gagatgattg
     2041 aagggctgcc cgcctctgat gcgctgacgc ctgccgatcg ctatctcgaa ctgttcgagc
     2101 atgttcagtc ggcgaaaatt ttccccgaca gtaaaacctt tcccgactgc gcacccaaaa
     2161 tggacccgct ggatatttta atccgctacc gtaaagtgcg ccgtcatcgt gattttgact
     2221 tgcgcaagtt tgttgaaaac cacttctggc tgccggaggt ctactccagc gagtatgtat
     2281 cggacccgca aaattccctg aaagagcata tcgaccagct gtggccggtg ctaacccgtg
     2341 aaccacagga tcacattccg tggtcttctc tgctggcgct gccgcagtca tatattgtcc
     2401 cgggcggccg ttttagcgaa acctactatt gggattccta tttcaccatg ctggggctgg
     2461 cggaaagtgg tcgggaagat ttgctgaaat gcatggccga taacttcgcc tggatgatcg
     2521 aaaactacgg ccacatcccc aacggcaacc gcacctatta tttgagccgc tcgcaaccac
     2581 cggtttttgc gctgatggtg gagttgtttg aagaagatgg tatacgcggc gcgcgccgct
     2641 atctcgacca cctgaaaatg gaatatgcct tctggatgga cggtgcagaa tcgttgatcc
     2701 ctaatcaggc ctatcgccat gttgtgcgga tgccggacgg ctcgctgctc aaccgttact
     2761 gggacgatcg cgacacgccg cgtgacgaat cctggcttga ggacgttgaa accgcgaaac
     2821 attctggtcg cccgcctaac gaggtgtacc gcgatttacg tgcgggggcg gcctccggtt
     2881 gggattactc ttcccgttgg ctgcgtgata ctggtcgtct ggcgagcatt cgtaccaccc
     2941 agttcatccc catcgatctg aatgccttcc tgtttaaact ggagagcgcc atcgccaaca
     3001 tctcggcact gaaaggcgag aaagagacag aagcactgtt ccgccagaag gccagtgccc
     3061 gtcgcgatgc ggtaaaccgt tacctctggg atgatgaaaa cggtatctac cgcgattacg
     3121 actggcgacg cgagcaactg gcgctgtttt ccgctgccgc cattgtgccg ctctatgtcg
     3181 gtatggcgaa ccatgaacag gccgatcgtc tggcaaacgc cgtgcgcagc cggttactga
     3241 cacctggcgg gattctggca agcgagtacg aaaccggtga acagtgggat aaacccaacg
     3301 gctgggcacc gttacagtgg atggcaattc agggatttaa aatgtacggc gatgaccttc
     3361 tgggtgatga aatcgcgcgc aactggctga agacggtgaa tcagttctat ctggaacagc
     3421 acaaactgat cgagaaatac catattgccg atggtgttcc tcgcgaaggc ggcggtggcg
     3481 agtatccgtt gcaggatggg tttggctgga ctaacggtgt ggtacgccgt ttaattggtt
     3541 tgtacggcga accataatat ttttacagcc agccgctaac ttcctgctgg ctgtaaaatt
     3601 atcctcttca ggaggagata tttaacatca ttgccgcctg ggtgcgattt ttgacttcca
     3661 gacggcgata cagtgattcc agatgcgctt ttaccgttcc ggtactgata ttcagcgccc
     3721 tgccgctctc tttatttgat tcgcccgccg ctaacatggt taaaatctcc cgctggcggg
     3781 cgcttaacga tttgagatct ttaatgtcct tttccggcgt cgttcgccag tctccaggca
     3841 gaaacatcat ccccatcgcc gcactattta ccgccaacgc aaatgtctcg acggttgaat
     3901 cacgaggcac aatggccagc acattaaaat gaataacttc ctgtaaccac cgtttgttgc
     3961 aatccgtcgc cgtaattaac accttaacct caggaaattg caccacggtt ttttgcagca
     4021 accagtagca aaactcacca tcctgatcgc catcgagcat cactaaggct tcagggtaac
     4081 tttccagctt ttgccataac tcgtctgcct gactggcccc ctgaatactc actcctggaa
     4141 tacgatgctg taaactgatt ttcattccat gaataaatat tgactgcctg tcaaacatga
     4201 ctatttgcat aactgaatct ccacctgaat acgttaaaaa gacttaagta gtggaagggt
     4261 attacccgcg agaaaaaata agaattcgcc atttggcggt ggccattcta cagagatgac
     4321 gcgtagaaaa tagttaccga tataaatagt tacagctaaa cgcctgaaat tacatgtcga
     4381 gggcact
//
LOCUS       DAZFJL010000209         4327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZFJL010000209 DAZFJL010000000
VERSION     DAZFJL010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.211"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..1155
                     /locus_tag="ACHJ8C_004459"
     CDS             <1..1155
                     /locus_tag="ACHJ8C_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO5554193.1"
                     /translation="WVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVF
                     SLALSLLYLLTLAVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSL
                     SNHAVGRLEVILVGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAY
                     AGFGMMANAADKVKDPEVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTA
                     VAQAASPLLGHVGYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKPLW
                     RQSTWGNIIVVVLIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPI
                     LIVGTLVMLLVIVGFIYSLWSQGSRALIWIIGALLLSLIVAMVMKRNKTV"
     gene            1186..1283
                     /locus_tag="ACHJ8C_004460"
                     /pseudo
     CDS             1186..1283
                     /locus_tag="ACHJ8C_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1280..1687
                     /gene="cueR"
                     /locus_tag="ACHJ8C_004461"
     CDS             1280..1687
                     /gene="cueR"
                     /locus_tag="ACHJ8C_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO5554194.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIEQHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(1688..2399)
                     /locus_tag="ACHJ8C_004462"
                     /pseudo
     CDS             complement(1688..2399)
                     /locus_tag="ACHJ8C_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589011.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2488..2694)
                     /locus_tag="ACHJ8C_004463"
     CDS             complement(2488..2694)
                     /locus_tag="ACHJ8C_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554195.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGING"
     gene            complement(2691..3113)
                     /locus_tag="ACHJ8C_004464"
     CDS             complement(2691..3113)
                     /locus_tag="ACHJ8C_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554196.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPASRIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNADATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(3199..4215)
                     /locus_tag="ACHJ8C_004465"
     CDS             complement(3199..4215)
                     /locus_tag="ACHJ8C_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO5554197.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGV
                     VQTPARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTTTEQGQKMNALTTDVAAQQQKERAQYDKQMQSLAQESAQAHEQIDS
                     LTQDAIQTHQQLSNTQKRVADNSQQINTLNNHFSSLKNEVEDNRKEANAGTASAIAIA
                     SQPQVKTDDLMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
BASE COUNT          974 a         1029 c         1125 g         1199 t
ORIGIN      
        1 tgggtcgcct ttgcttttgg cggtattgtg gcgatgtttt ccggttatgc ctatgcgcgt
       61 ctgggggcga gctatcccag caatggcggt attatcgact tctttcgtcg cggattaggc
      121 aacggcgttt tttcgctggc gctctcgtta ttgtacctgt tgacgctggc ggtgagtatc
      181 gccatggtcg cccgtgcttt tggcgcttat gccgtgcagt ttttgcatga aggcagccag
      241 gaggagcacc ttattttgct ctacgcattg gggatcattg cggtgatgac gcttttcaac
      301 tccttaagca accatgcggt agggcggctg gaagtgatcc tcgtcggcat taaaatgatg
      361 atcctgttat tgctgattat tgccggtgtc tggtcgctgc aaccggcgca tatttccgtt
      421 tctgcgcccc ccagctccgg ggcgttcttc tcctgtatcg ggataacctt ccttgcctat
      481 gcgggctttg gcatgatggc gaacgcggcg gataaagtga aagatccgga ggtcataatg
      541 ccacgggcat ttctggtggc gattggcgtt accacgttgc tttatatctc gctggcgctg
      601 gttttgctta gcgatgtatc ggcattagag ttagaaaaat atgccgatac cgccgtagcg
      661 caggctgctt ctccgctcct cgggcatgtg gggtatgtga tcgtcgtcat cggcgcttta
      721 ctggcgacgg cttcagccat caacgcaaac ctgttcgccg tgtttaacat catggacaac
      781 atgggcagcg aacgcgaact gccgaagcta atgaataaac ctctgtggcg gcagagtacc
      841 tggggcaaca tcatcgtcgt ggtgttgatt atgctgatga cggcggcgct gaatttaggc
      901 tcactcgcca gcgttgccag cgccaccttt ttgatctgct atctggcggt gtttgtggtg
      961 gcgatccgcc tgcgtcatga tattcacgcc tcgttgccga ttcttatcgt tggtacgttg
     1021 gttatgttgt tggtgatcgt tggctttatc tacagtctct ggtcccaggg cagccgtgcg
     1081 ctgatatgga ttattggcgc actcttactc agccttattg tggcaatggt catgaagcgc
     1141 aataaaaccg tataacatct ctctgtgcgc agtacttcct gtattattgt ggtggcggtc
     1201 gatattcgca ctggcaaaaa aacgtgcttg aatatttgtt gaaacccttt aacaaagcac
     1261 aggaggcgtt gcgcgaacga tgaacattag cgatgtagca aaaattaccg gcctgaccag
     1321 caaagccatt cgcttctatg aagagaaggg gctggtgacg ccgccgatgc gcagcgaaaa
     1381 cggttatcgc acctacacgc agcagcatct caacgaactg accttactgc gccaggcacg
     1441 ccaggtgggc tttaacctgg aagagagcgg cgaactggta aatctgttta acgatccgca
     1501 gcgtcacagc gccgacgtca aacgtcgtac gctggagaaa gtagcggaga tcgaacagca
     1561 tatcgaagaa ctgcaatcca tgcgtaacca gttgctggcg ctggcgaatg cctgccccgg
     1621 tgatgacagc gccgactgcc cgattatcga aaatctctcc ggctgctgtc atcatcgggc
     1681 ggggtgatta aggcttgcga aaatagtcgt ccattatttt cgtcagctcc ggacgttccc
     1741 cgtcatcatt gatggcaaaa tccagaaaaa aagcatctgc ttgcggcgtt cggttttgcg
     1801 ccatcaaccg gaaggtgagg gggcctgacc aggtttagga tgggctgatg tgcttatcag
     1861 ggccgaaact ctcccatgcg ttgggcggta atgttttgca cgcggtgagt tttggtatgt
     1921 ggtattgcag ctctgttttg tgcagacatt ccccgccagg aaataccagg gtttgttgct
     1981 catcattaaa atgggttgcc gtgctgagca ccagtgtttt gagtggtcgc ccttccagcg
     2041 cggcggcagt agccttatgg tgtccgtcga gcagaacgca gagataggaa tcagggagat
     2101 accaggccag agcgcgggcg attccagttt tcgagagcag acaaaaaccg ggcggctccc
     2161 tgttcatcct ccgcaaaacc gctgtagaca aattccgcac aggtagggca accagcctcg
     2221 gaccagaaac gtattaacgg gacatccttg ccagcagagc gaatggataa gaccgtgcga
     2281 gtgcggatcg taaatcacac attgacgcag aaatccttcc cggacttcac cgaaaaaatg
     2341 acgcccgcag cggtattttt cagcagacgt acttcaggtt ttcgacgggg aaaaagcatg
     2401 gccactcctt agccaaaatt tcagttccct gaactgtgcc agaaaattac gctggtgact
     2461 attgctctgg aaatggaaac ggtgtcctta cccattaatc cccgtttgta ccgaaagaat
     2521 atgctgcaaa ctttctacca taatgcgccc cgactgaaat aagccttcct ggatttcatt
     2581 gttaacattc acagcgtgaa ccagggaggc taataacacc acttgcaaga aggcaaaaat
     2641 aaacgcaccg ataatcataa tatgcgtcag ggggattttc ctttgctcct tcattttaaa
     2701 taacccacaa actggccttc tttcattaag gcgacttttc ctgtgtgatg ataaacaatg
     2761 acattataca gacccgactg ataaaccgtg gcatcggcat tatcttcatc tatggcacat
     2821 aatgaatcat tgagataagc aaagttctta ctactcattc taaaggtcat gccaaatcct
     2881 ttataaatgg ctttttttgc cgcctgggca tgttgtgcgg cttctgcatt tttaaatgcc
     2941 tgcgcttcag aagcacttaa aatagccgtc tggcgctgct gttctgcgag ataacaggtg
     3001 tcatggctga cgccctgcgc ctgacaatct gcgatgcggg aagcgggtga gctacagcca
     3061 gccagtaata taaccgccag cagggaaaag gatttaatgg agctattcat cattgttaac
     3121 cttaaataat gtgcgaatta atgaagaggt gaaacccggc aagcttcctt gctcttattt
     3181 atattgagga ttgaaatatt agaacgaata tcctacgccg acacctgcgc cgaaatcaga
     3241 ttgcgtatcc gcagaaatac cggctttaag taccgtatgc gttccggcat taaaagatgt
     3301 tccgacagac accgcagatt caccgttgaa ggttcccgct cccgctgaca ccatcatcaa
     3361 gtcatcggtt ttcacctgtg gttgtgaggc gatagcgatg gctgatgcag ttcccgcatt
     3421 ggcttcttta cggttgtcct caacttcgtt tttcagcgaa ctgaaatggt tattgagcgt
     3481 gttaatttgt tggctgttat ctgcgacccg tttttgcgtg ttgcttaact gctgatgcgt
     3541 ttggattgcg tcttgtgtca ggctgtcaat ttgttcatgt gcctgggcag actcctgcgc
     3601 cagactttgc atttgtttat cgtattgagc cctttctttt tgctgttgtg ctgccacatc
     3661 ggttgtcagc gcattcattt tctgcccttg ttccgtggta gtgcgctgat tagcctcatt
     3721 ttgagccgcc agtctggcat taatcatctc ctgataatgc gcggtatttt gcgtatctcc
     3781 agtcgcggcg agcgtttttt gtgtggctac gatctgcgcc tgttgctctg taatttgcgt
     3841 ttgctgggcg tcagttaccg cagactgtcg ggcgttgatc atgctctgat aacgggcagg
     3901 tgtctgtaca acacctgtag cggcaacggt tttttgtcct gcgtcaggat ctgtctggac
     3961 agtgggagtc tgagtggtat caggtgtttc agaagggatg tagtgatgtt cagtcaaata
     4021 ggtatacgca gcggggctgt tgtctgtgcg ttctaaaaag cgggtaatct tctgctcaat
     4081 ctccgattgc gacatgccgg agttaatggc tgaaagctct gttgccgccg cttcaatggt
     4141 atcaccagct aaaacaacag ggctggatgt tgctgaaatt atgagtgcca gtaaagctac
     4201 gtttacagtt ttcattcatt ttctcttgtt caaaataagt cgcattaatg tttctataag
     4261 aaacaaataa tattgtttta agaaataaac tgcgttgcaa tttgatcgaa atctacgccg
     4321 ccatttg
//
LOCUS       DAZFJL010000210         4305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZFJL010000210 DAZFJL010000000
VERSION     DAZFJL010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.212"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            1..105
                     /locus_tag="ACHJ8C_004466"
                     /pseudo
     CDS             1..105
                     /locus_tag="ACHJ8C_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
     gene            complement(158..328)
                     /locus_tag="ACHJ8C_004467"
                     /pseudo
     CDS             complement(158..328)
                     /locus_tag="ACHJ8C_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(325..1227)
                     /locus_tag="ACHJ8C_004468"
     CDS             complement(325..1227)
                     /locus_tag="ACHJ8C_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO5554198.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QNTEDVQFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDVQNCLMLHELWLQSG
                     TGQRRWEGLPDEVRETITVHFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLNVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISRQGENFIQVDF
                     DTPWCQPESNVVAELSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLWGELEWSSP
                     TDDDDLPEVTGPAWIVDKVAHYGG"
     gene            1666..2625
                     /locus_tag="ACHJ8C_004469"
     CDS             1666..2625
                     /locus_tag="ACHJ8C_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO5554199.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDGKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNITHVIDSEVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            2625..3020
                     /locus_tag="ACHJ8C_004470"
     CDS             2625..3020
                     /locus_tag="ACHJ8C_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO5554200.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSASINPKDDFEEQTRRNLSMLKK"
     gene            complement(3389..>4305)
                     /locus_tag="ACHJ8C_004471"
     CDS             complement(3389..>4305)
                     /locus_tag="ACHJ8C_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element IS1414 family transposase"
                     /protein_id="HFO5554201.1"
                     /translation="RITGMDNQILSLYAKGMTTREIATAFKELYDADVSPALISKVTD
                     AVMEQVVEWQNRPLDAVYPIVYLDCIVLKVRQDSRVINKSVFLALGINIEGQKEQLGM
                     WLAENEGAKFWLNVLTELKNRGLNDILIACVDGLKGFPDAINTVYPKARIQLCIVHMV
                     RNSLRFVSWKDYKAVTRDLKAIYQAPTEEAGQQALEAFAAAWDSRYPQISRSWQANWP
                     NLATFFAYPTDIRKVIYTTNAIESLNSVIRHAIKKRKVFPTDDSVKKVVWLAIQSASQ
                     KWTMPLKDWRMAMSRFIIEFSDRLDGHF"
BASE COUNT         1252 a         1089 c          974 g          990 t
ORIGIN      
        1 agcagtagca gtagcaggaa caacaacatc agtaacagac aaatgacagg catgaagaaa
       61 cagaagcccc catcagaacg tccgcgtaat gaaaggggcg gcgcgtcagc gcggcccctt
      121 tcagcgccac cggcgcgcgg tcttttcccg gcgcgggcag ccgggagcgg taaaagcgcc
      181 cgtcccgtgc ccgccggacg cgggacactg accacaggca gcgtttatgc cgcgccgcgt
      241 cagccggcgc agtaaaaaga ggagcacggc gacatccttt accggaaaag agacgggcgg
      301 ttgcccgccc gtctccgtca tacttcatcc gccataatgc gccaccttgt cgactatcca
      361 cgcaggtccg gtgacttccg gcaggtcatc gtcatctgtc ggggaagacc attccagttc
      421 cccccacagc acatcaacga gctctccgcg ctcatacaac tgccagccac agaaattgca
      481 gccctgttcg gcgtaccagt gctccagtgt gcagccgaaa cggcggctta attctgcaac
      541 aacgttgctt tccggctgac accacggcgt gtcgaaatcc acctgaatga agttttctcc
      601 ctgacggctg atattcacct cataccccac aggccacttc acgccatact gttccgtata
      661 ccagtgatac gcagaaggaa caccgttcag aacaccaccg ttaaagccat tcacttcaac
      721 attcaggcgg gtgggcagaa ccgtcagcag gtcaaacggc atggtttttt ccggcagtac
      781 attgtcacac agacggttcc accacaccga tacatcctcg ttactccaga agccacacca
      841 gtcacctctt ttggcggtga aatgcacggt gatggtttcc ctgacctcat ccggtaatcc
      901 ttcccagcga cgctggccag taccactctg cagccagagt tcatgcagca tcaggcagtt
      961 ttgtacatcc agtagcacac cgttctgcag gtgtgtcagc cagcgggtga acgcgatatt
     1021 ctccggcgat acaacaccac gtccggcagc cgtcagtccg gggcacggtt caaactgcac
     1081 atcttcagtg ttctgcagaa gtccggcact tccggccaga aacagctgaa taccttcatt
     1141 tgtggcgcgg cgataaaacg gtgtgactgc accgctggcc agtcgtttaa tctcagcaat
     1201 ctgtgccggt tcaccagaaa aatgcatacg atttgagcac cagtttggca tgggaattct
     1261 cctctcacat gcggcctcca tcaggccgtg ccgcacctgt aaccgggcga ccgcatacga
     1321 atgcaggtgc ggccccgaag ccgcagcgcg tggaggagga acgacgaaac gtgaccgggt
     1381 gaggaggccg gcgacagcga cacacttgca tcggatgcag cccggttaac gtgccggcac
     1441 ggcctggtta agtagtttcc gtctcagttt gtgacgcaaa atgagaaact aacaacaact
     1501 caaattgagt cacaataaga acacaaggag actctaaaag agccacatta cttgacatta
     1561 acccaaaatg aaacacaata agaaataaca aaaactcaaa acgatgcatc gacataaaag
     1621 atgctcaatt tgagacaaac acaaactcaa aacgaggcaa aaagaatgct aaaagtatca
     1681 tgcgatgatg gttcaactaa cgtaaaactg gcctggcttg aagatggcga agtaagaacc
     1741 agtctgtcag gaaacagctt taaagaaggc tggaacccgg gactgtttaa tgccggcaaa
     1801 gtttacaatt acgtcgtgga tggaaaaaaa tacacctatg atttgggcag cactgctgtc
     1861 ataggtacaa cacatgtcag ttatcaatac agcaccacca atctgcttgc catacatcat
     1921 gcactgctga catccggcct tcagccacag gatgtagaac tgacagtaac attacctgta
     1981 accgaattct ttgataatga caatcaacca aatgaagaaa gaatagaaag aaaaaaggca
     2041 aatgttctcc gtgagatatc tctgaataaa ggggaaacat ttaaaattaa aaaagttaat
     2101 gtaatgcctg aatctttgcc ggctgcattt gaatcactaa aaaaagataa agtaaacaaa
     2161 cttgaacgca gcctgattat cgatcttgga ggaaccactc tggattgtgg tttaattctc
     2221 ggtgcatttg aaggcatttc tgaaatccgt ggctattcag aaatcgggac atcccgcata
     2281 actcataccg tcatgaatgc actaacaaaa gcctccacac cctgcaatta ttttattgct
     2341 gatgagttaa taaaaaacag gcatgataat gaatatctcc agacattaat aaatgatgtg
     2401 gctgaaataa aaaatattac tcatgtaata gacagtgagg tgaaatctct ggcagaaagc
     2461 atcaggcagg aaatttcaac attctcagga atgaaccgaa tctacctcac aggcggggga
     2521 gccgagctta tttaccctca cataaagcaa tacttcccga atctgaaagt gaacaaggta
     2581 gatgaacctc agtttgcatt ggtaaaagca atggtgcatg cgtaatggaa agcagtgatc
     2641 caaaaaaaag aaagaaagtt gtggcatatc tgcacccggc actttatccg caggacaacc
     2701 tgacacaaca aacaatagac tctctcccag tacaaatgcg gggagatttc tacagacaat
     2761 cccttatttg cggagcagct ctttattctg ttgacccccg actgttaaca ctaatcagcg
     2821 gttttttcag tgagaaaatc actgcagaaa atctggtgaa acttatagaa caaacaacag
     2881 gatacacatc gacatcaatt gatataagtg tgttaaaaaa catcatagag gcatcttcag
     2941 aaaacaaatc agcgagcatc aatccgaaag atgatttcga agaacaaacc agacgcaatc
     3001 tgtctatgtt aaaaaaatga caaagagggg cagaatatat tctgcccact ctaattttca
     3061 caatacttat gttctgtaaa gcatccaact gtagttgaaa aactcaaatg tctttgagtt
     3121 taatattttt tacatctatc attcctaaat aagaatgaac actaacaaaa ggggtttata
     3181 ccaataggag gtataggaac aggagcatca tgtagctctc gaagaagcag ctcaaaatta
     3241 ttatgatatt cggctccatc actcaaattg tagtaaaatc gtcctgacaa tgcatctggc
     3301 aaagagaccc cattagttag atttcttata attggaataa attttataaa tcccgagcct
     3361 gtttaggatt ctgtgtaaat gccttttctc agaagtgacc gtccaggcgg tcactgaact
     3421 cgataataaa gcggctcatt gccattcgcc agtccttcaa cggcatcgtc catttctggg
     3481 acgcagactg gattgccagc cacaccactt ttttcaccga gtcgtctgtc gggaacactt
     3541 tacgcttttt gatcgcatgg cggatcacgc tgtttagcga ctcgatggca ttcgtcgtat
     3601 agatcacttt gcggatgtcc gttggataag cgaagaacgt ggcaagattc ggccagttag
     3661 cctgccagct tctgcttatc tgaggatagc gactgtccca ggccgcagcg aacgcttcca
     3721 gtgcctgctg acctgcctct tccgtgggag cctgataaat cgctttcagg tcgcgagtga
     3781 cggctttgta gtccttccat gacacgaagc gcaggctgtt gcgcaccata tgcacgatgc
     3841 ataactggat gcgggccttc ggatatactg tgttgatggc atccgggaag cctttcaggc
     3901 catccacaca ggcgatgagg atatcgttca gaccgcggtt tttcagttca gtcagcacat
     3961 tgagccagaa cttcgcccct tcattttcgg ccagccacat acccagctgt tctttctgac
     4021 cttcgatatt gatgcccagt gccaggaaca ccgatttgtt gatgacgcgg ctgtcctgcc
     4081 gaactttcag gacgatacag tcaagataaa caatggggta aacagcatcc agtggtctgt
     4141 tttgccattc tacaacctgc tccatcacgg catcggtgac ctttgatatc agtgccggtg
     4201 aaacatctgc gtcatacagt tctttgaacg cagtagctat ctcacgggtg gtcatccctt
     4261 tggcatacaa cgagaggatc tggttatcca tcccggtaat acggg
//
LOCUS       DAZFJL010000211         4274 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZFJL010000211 DAZFJL010000000
VERSION     DAZFJL010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.213"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..183
                     /locus_tag="ACHJ8C_004472"
     CDS             <1..183
                     /locus_tag="ACHJ8C_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5554202.1"
                     /translation="ATSTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEI
                     STADEGDGGQVVVIGR"
     gene            244..3726
                     /locus_tag="ACHJ8C_004473"
     CDS             244..3726
                     /locus_tag="ACHJ8C_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO5554203.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWTDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     IAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LAPGNYRLTVRAVNARGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPAITSGGNP
                     PAFSLTPDGRLTAKNADISGSVNANSGTLNNVTINENCQIKGKLSANQIEGDIVKTVS
                     KSFPRTSTYASGTITVTISDDQKFDRQVMIPPVLFRGGKHENFNSNNQQSYWYSTCRL
                     RVTRNGQEIFNQSTTDTQGVFSSVIDMPAGQGTLTLTFTVSSSGANNWTPTTSISDLL
                     VVVMKKSTAGISIS"
     gene            3793..>4274
                     /locus_tag="ACHJ8C_004474"
     CDS             3793..>4274
                     /locus_tag="ACHJ8C_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5554204.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGLVTSFSYANAKDEQKTHYSDTRWHEDSVRNRWFSMMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     G"
BASE COUNT         1026 a         1057 c         1309 g          882 t
ORIGIN      
        1 gcgaccagca cggataacgg taagcagaac acctatttct cgtcactgga taacatggtt
       61 gctcagggca atgttttgcc tgttctgtac ggtgaaatgc gtgtggggtc gcgggtggtt
      121 tctcaggaga tcagcacggc agacgaaggt gacggtggtc aggttgtggt gattggtcgc
      181 tgatgcaaaa tgttttatgt gaaaccgcct gctggcggtt ttgtcgttta tggagcatga
      241 cgaatgggta aaggcagcag taaggggcat accccgcgcg aagcgaagga caacctgaag
      301 tccacgcagt tgctgagtgt gatcgatgcc atcagcgaag ggccgattga aggtccggtg
      361 gatgggttaa aaagcgtgct gctgaacagt acgccggtgc tggacagtga ggggaatacc
      421 aacatctctg gtgtcacggt ggtgttccgg gcaggtgagc aggaacagac accgccggag
      481 ggatttgaat cctccggatc cgagacggtg ctgggtacgg aagtgaaata cgacacgccg
      541 atcacccgga ccatcacgtc ggcaaacatc gaccgtctgc gctttacctt cggtgtgcag
      601 gcactggtgg aaaccacctc aaagggtgac aggaatccgt cggaagtccg cctgctggtt
      661 cagatacagc gtaacggtgg ctgggtgacg gaaaaagaca tcaccattaa gggtaaaacc
      721 acttcacagt atctggcctc ggtggtggtg gataacctgc cgccgcgccc gttcagtatc
      781 cggatgcgca ggatgacgcc ggacagcacc acagaccagc tgcagaacaa aacgctctgg
      841 tcgtcataca ccgaaattat cgatgtgaaa cagtgctacc cgaacacggc actggtcggc
      901 gtgcaggtgg actcggagca gttcggcagc cagcaggtga gccgtaatta tcatctgcgc
      961 gggcgtattc tgcaggtgcc gtcgaattat aacccgcaga cgcggcaata cagcggtatc
     1021 tgggacggaa cgtttaaacc ggcatacagc aacaacatgg cctggtgtct gtgggatatg
     1081 ctgacccacc cgcgctacgg catggggaaa cgtcttggtg cggcggatgt ggataaatgg
     1141 gcgctgtatg tcatcggcca gtactgcgac cagtcagtgc cggacggttt tggcggcacg
     1201 gagccgcgca tcacctgtaa tgcgtacctg accacacagc gcaaggcgtg ggatgtgctc
     1261 agtgatttct gctctgcgat gcgctgtatg ccggtatgga acgggcagac gctgacgttc
     1321 gtgcaggacc ggccgtcgga taaggtgtgg acctataacc gcagtaatgt ggtgatgccg
     1381 gatgatggcg cgccgttccg ctacagcttc agcgccctga aagaccgcca taatgccgtt
     1441 gaggtgaact ggactgaccc ggacaacggc tgggagacgg caacagagct tgtggaggac
     1501 acgcaggcca ttgcccgtta cggtcgtaac gtcacgaaga tggatgcctt tggctgtacc
     1561 agtcgggggc aggcacaccg tgccgggctg tggctgatta aaacggagct gctggaaacg
     1621 cagaccgtgg acttcagcgt gggcgcagaa gggcttcgcc atgtaccggg cgatgtcatt
     1681 gaaatctgtg atgatgacta tgcggggatc agcaccggtg ggcgtgtgct ggcggtgaac
     1741 agccagaccc ggacgctgac gctcgaccgt gaaatcacgc tgccatcctc cggtaccacg
     1801 ctgataagcc tggttgacgg aagtggcaat ccggtcagcg tggaggttca gtccgtcacc
     1861 gacggcgtga aggtaaaagt gagccgtgtt cctgacggtg ttgctgaata cagcgtatgg
     1921 gggctgaagt tgccgacgct gcgccagcga ctgttccgct gcgtgagtat ccgtgagaac
     1981 gatgacggca cgtatgccat caccgccgtg cagcatgtac cggaaaaaga agccatcgtg
     2041 gataacgggg cgcactttga cggtgaccag agcggcacag tgaatggtgt cacgccgcca
     2101 gcggtgcagc acctgatcgc agaagtcact gcagacagcg gggaatatca ggtgctggcg
     2161 cgctgggaca cgccgaaggt ggtgaagggc gtgagcttcc tgctccgtct gaccgtaaca
     2221 gcggacgacg gcagtgagcg gctggtcagc acggcccgga cgacggaaac cacataccgc
     2281 ttcacgcaac tggcgccggg gaactacagg ctgacagtcc gggcagtaaa tgcccggggg
     2341 cagcagggcg atccggcgtc ggtatcgttc cggattgccg caccggcagc accgtcgagg
     2401 attgagctga cgccgggcta ttttcagata accgccacgc cgcatcttgc ggtttatgac
     2461 ccgacggtac agtttgagtt ctggttctcg gaaacgcgga ttaccgatat caggcaggtt
     2521 gaaaccacag cccgctacct tggcacgggg ctgtactgga tagccgccag tatcaatatc
     2581 aaaccgggcc atgattatta cttttatatc cgcagtgtga acaccgttgg caaatcggca
     2641 ttcgtggagg ctgttggtca gccgagtgat gatgcatccg gttatctgga ttttttcaaa
     2701 ggcgagatag ggaaaaccca tctggctcag gagctgtgga cgcagattga taacggtcag
     2761 cttgcgcctg acctggctga aatcaggacg tccattacag atgtcagtaa tgaaatcaca
     2821 cagaccgtca ataagaaact ggaagaccag agtgcggcaa ttcagcagat acagaaggtt
     2881 caggttgata caaataataa cctgaacagc atgtgggcag tgaagctgca gcagatgcag
     2941 gacggacgcc tctatattgc gggtatcggt gccggtattg agaacacccc tgacggcatg
     3001 cagagtcagg tgctgctggc ggcagacagg attgcgatga ttaatcctgc aaatggcaac
     3061 acaaagccga tgtttgttgg tcagggtgat cagatattca tgaacgaagt gttcctgaag
     3121 tatctgacgg ctcctgccat taccagtggc ggtaatcctc cggcattttc cctgacgccg
     3181 gacgggcgac tgacggcgaa aaatgcggat atcagtggca gtgtgaatgc gaactcaggg
     3241 acgctcaaca acgtcacgat aaatgagaac tgtcagatta aggggaaact gtccgccaac
     3301 cagattgaag gcgatattgt caaaacggtc agcaagtctt tcccccgcac gagcacttat
     3361 gccagcggca ccattacggt cacgattagt gatgatcaga agtttgaccg acaggtcatg
     3421 ataccgccag tgttattccg cggtggtaag catgagaatt tcaacagtaa taaccaacag
     3481 tcatactggt attcaacctg ccgattaaga gtgacccgca atggtcagga gatttttaat
     3541 cagtccacga cggatactca gggcgtattt tcctcagtta tagatatgcc tgccggacag
     3601 gggacgctga cactgacatt caccgtatct tcatcaggag cgaataactg gacaccaaca
     3661 accagtatca gcgatctgct ggttgtggtg atgaaaaaat ccacagcagg tatcagtatc
     3721 agctgaattt tttaacccag aacgggcgtc agaaatgacg ccttttttat tgcagaaaag
     3781 cgagaggtaa ttatgcgtaa actttatgcc gccattttgt ctgcagccat ctgtctggcc
     3841 gtatccggtg cgcctgcatg ggcgtctgag cagcaggcca cgctgagcgc ggggtatctt
     3901 catgcccgga cgaacgctcc cggtagcgat aatcttaacg ggattaacgt gaaataccgt
     3961 tatgagttta cggacacgct ggggctggtg acgtcattca gttatgccaa tgccaaagat
     4021 gagcaaaaaa cgcattacag cgatacccgc tggcatgaag attcagtgcg taaccgctgg
     4081 ttcagcatga tggcggggcc atctgtacgc gtgaatgaat ggttcagcgc gtatgcgatg
     4141 gcaggtgtgg cttacagccg tgtttcgacg ttctccgggg attatcttcg cgtaactgac
     4201 aacaagggga aaacgcacga cgtgctgacc ggaagtgatg acggtcgcca cagcaacacg
     4261 tctctggcgt gggg
//
LOCUS       DAZFJL010000212         4257 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFJL010000212 DAZFJL010000000
VERSION     DAZFJL010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.214"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(114..1004)
                     /gene="metF"
                     /locus_tag="ACHJ8C_004475"
     CDS             complement(114..1004)
                     /gene="metF"
                     /locus_tag="ACHJ8C_004475"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO5554205.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPAELRII
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLRRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(1386..2939)
                     /locus_tag="ACHJ8C_004476"
     CDS             complement(1386..2939)
                     /locus_tag="ACHJ8C_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO5554206.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNIFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHNFTVKNFELKTIYADEWKPDQRTKQVIDGWNKKLDEVVQQTVAQSPV
                     ELKRAYGESASLGNLAADALLAAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMELTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKP
                     IEDATVYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQL
                     NGMRVKDIK"
     gene            3072..4196
                     /locus_tag="ACHJ8C_004477"
     CDS             3072..4196
                     /locus_tag="ACHJ8C_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HFO5554207.1"
                     /translation="MSTLFTINACKTFGCRNLGLASSEDYSWPDYKLGYPALHCRACG
                     SYPPLFDEQQFRDWLSVHLSTYAIEKGHFCPVCYGTDMICYGHNPQGSQRIQCRNCKK
                     VWTPKKYQKEITHPQAIETVQLFIPFQGASAVQKLYVLVSLDATRGNILHLSTNYTQH
                     QTGDSLRYSYKGNTEPTMHHHDIVQKVDMREAQFLRRSQFDEIQYGSAVLKRNGKGAI
                     LRPVITAHGHFRILKIRYPDVKTHIISHECFLRGAIITAWADQFRQQQGELWFVEEEI
                     SDSNADTPWHFKGTTYHGWWQNQWQRWEQGNNCKMVCLLTGASLERGANVSLATSRCF
                     ITWLTDQHDFTQSALLSAGRVTQMLTSLALKYNESLTPSC"
BASE COUNT         1106 a         1065 c          968 g         1118 t
ORIGIN      
        1 gagggagaaa acaaggctga taaccttatt ttcccgccct catttcgagg cagcattttg
       61 tgctctgttt aaaatttgtg atcactgtgt gattttcaca aaaggcacac tatttataaa
      121 ccaggtcgaa cacccagtgt atggcaaatc gcgtaactca tttcagcgcg gttaagcgta
      181 tagaagtgga aatctttcac tccttcacgg cttaaaatct tcaccatatc catggcaata
      241 ttcgcgccca ccagcttgcg ggtttcagca tcgtcatcca gcccgtcgaa catttgtgcc
      301 atccacgccg gaatacgcac gttggtcatg tcggcaaatt tcttcgcctg tttaaagttc
      361 gaaaccggca aaattcccgg aatgatttcc acatcaatgc ctgccgagac gcagcgatcg
      421 cgaaaacgca ggtagctttc gacatcgaag aagaactgag taatggcgcg gttggctccg
      481 gcatccactt ttcgtctcag gttgagtaaa tcggcctggg cacttttcgc ttctggatgt
      541 acttccggat acgccgccac ggagatatcg aaatctgcca cttcttttaa cagtgtcacc
      601 aggtcagaag catacatttc tggcttacca cttcccggcg gcaggtcgcc acgcagcgcc
      661 acgatatggc gaataccgtt attccagtag tcgcgtgcaa tgatccgcag ctcggcaggc
      721 gtcgcatcaa tgcaggtaag atgcggtgcc gcttccaggc cggtgcgatc tttaatgcct
      781 ttaataatgc tgtgcgtacg gtcgcgctcg ccggagtttg cgccataggt caccgataca
      841 aacttcggtt tcaggctgct aaggcgatcg atggagttcc acagggtctg ctccatttca
      901 ctggtacgcg gcgggaaaaa ttcgaacgaa acgttaatct gcccctggac ttctgccaga
      961 ctctgattca gggcatcccg ctggctggcg tgaaaaaagc tcatacctta cctcatcaat
     1021 cgcttgtcgt tatatgttgt gttgtgcaca tctatccgtt tagacgtcca gatgtaaaga
     1081 tgaaggaaaa gccgaagggc gtcaaccgaa aaataaccaa tgatggtgag gaaaaatcag
     1141 ggaagatgaa aaaaactcat cttaattaga ggcgataaaa gatttctcta tagcgcaatc
     1201 cacctcgcac gttttgactc atattctgac aacacagcga cacctgggct aattcgaaaa
     1261 acaacgtcct gccaccggat ggtgatgtta aggcatctga ttcggcctgc gccctcgcgt
     1321 aggccgaata caggatctgc gcaatcaacg ggtaacaaac agttgagtca attcagtgcg
     1381 cacttttatt tgatatcctt cacgcgcata ccgttgagct gttcatccgt aatggtgttg
     1441 cccgctttga agtaatcaac caccgcgtga taaacgtaat aaccgcccgt tgtgttacgc
     1501 gctttccctt cggtaaaggc ggtaaaacca tctccaccat cagcaaggaa actctgagta
     1561 gcaatgtgat aaaccgtcgc atcttcaatg ggtttgccat tcagagtcag tgtgattacc
     1621 cgctgaccaa ccggcttgct gctgtcgtac ttcatttcca ggcctttcga tacctgtaaa
     1681 acgccattgc ttaaacttgc gccgtgttcc atcagactgc gtaattgttt acccgtgagt
     1741 tccatcgtca ccagttcgtt ggggaacggg aacgtactga tgacgccgcc catcgtgatc
     1801 gcccctgcgg ggatctcatt gcggatacca ccagagtttg ttaacgccag ttgggtattt
     1861 ttacccgctg ctgccagtaa agcgtcagcc gccaggttcc cgagggaagc cgattcacca
     1921 taggcacgtt ttaattcaac cggcgattgc gccaccgttt gttgcaccac ttcatcaagc
     1981 tttttattcc agccgtcgat cacctgtttc gttcgctgat cgggcttcca ctcgtcagcg
     2041 taaatggttt taagctcgaa gtttttcacc gtaaaattat gcggcttctc tttgtagtcg
     2101 agaaccagtt tgcccacatc aatcccgccg ctgtcagtgg agaggatgag cgtattgccg
     2161 actttaatcg gttccggcgt acccacatgt gcatgtccgg tgatcaaaat atccagccct
     2221 ttcacctgac ttgccgtctg aatatcttta tccaacgcgc gacgcacatc tgtgcctccc
     2281 atactggact ggcgggctgg aacaccttcg tggatcaggg cgacggtcaa atcaaccttg
     2341 cctttgagtt catcgatata acgttgtagc catttaattt catcgcgcgc ttcaataccc
     2401 acgcgcgtgg cagcagatac cgtatcattg aaggcaaata ctccgtgcaa acctataacg
     2461 ccaattttca cgccgtcttt ttcgatgatg gtatagggct tatcccagaa tgatttactg
     2521 ctgttctgat aaaaaatatt gccctgcaca atagggaatt ttgcctggct caactgtaat
     2581 aatgtgttgt cccagccgtg gtcaaattca tggttgccta ttgtgacggc atcaaacggc
     2641 atagtattca taatatcgat aatagcctta cctttggtca ggctgctgat atatggcccg
     2701 gtaaaatagt cgcccgcatc aaaaaaccat gtcgctttat tttttgcttt ttcttgctta
     2761 accagcgtag tgatatttgc ccagccgcca atatcccgtt taccgtcagc gatccacggc
     2821 actttataag gttctacatg cgcatggagg tcgttggtat aaataatagt gacatcttta
     2881 gcgcaagccc agaacggcag tgttaatgcg atacccgcag caagtatttt aattttcata
     2941 aacaatccct ttcagtgaga taaaggattg ctacattacg ttctgaaaga cttatttatg
     3001 tgagagtaat cacatccgca ggggatagtt gccggacaaa atcaacgacc ttgtgaaatc
     3061 gttggtgata tatgtcgact ctgtttacga ttaatgcctg taaaacgttc ggttgccgaa
     3121 atttgggatt ggcctcatcg gaagattaca gttggccaga ctataaactg ggttatccgg
     3181 cactgcactg tcgggcatgt ggcagttacc cgccgttgtt tgacgagcaa caatttcgtg
     3241 actggttgtc agttcacttg tctacttatg caattgagaa aggccatttc tgcccggtat
     3301 gttatggcac agacatgatt tgttatggtc acaatccaca gggttcgcaa cgcattcaat
     3361 gccgtaactg caaaaaagtc tggacgccta aaaaatatca gaaagaaatt acgcatcccc
     3421 aggccattga aaccgttcaa ctgttcatac cctttcaagg ggccagcgca gtacaaaaac
     3481 tgtatgtttt agtcagtctt gacgccactc gtggcaacat tcttcatctc tccacgaatt
     3541 atacgcaaca tcagacagga gacagtctgc ggtacagtta caagggcaat acagaaccaa
     3601 ctatgcacca tcacgatatc gtgcagaagg tagatatgcg tgaagcgcaa ttcttgcgcc
     3661 ggagccagtt cgatgaaatt cagtatggca gtgctgtact caagcgcaac ggtaagggag
     3721 ccatactacg cccggttatc acggcacacg ggcatttcag aatactgaaa atccgctatc
     3781 cagatgtcaa aacgcatatt atttcacacg aatgttttct aagaggcgca attattacag
     3841 cctgggcaga tcagttccgc caacaacaag gtgaactttg gttcgtagaa gaagaaatca
     3901 gtgacagtaa cgctgatacg ccctggcatt ttaagggaac gacataccat ggttggtggc
     3961 aaaatcagtg gcagcgctgg gagcagggga ataattgcaa gatggtctgc ctactcacgg
     4021 gagccagctt agaaaggggc gcaaatgttt ctctggcaac cagtcgttgt tttatcacat
     4081 ggctgacaga ccaacacgac tttacccaaa gcgcgttatt atccgcaggt cgcgtaacgc
     4141 aaatgctgac ctcactggcg ttaaaataca atgaatcgct cactccatct tgttagtcaa
     4201 tacactcgac aaacgaccat aaattaagag agatcaccac ctttcattta attaaat
//
LOCUS       DAZFJL010000213         4247 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZFJL010000213 DAZFJL010000000
VERSION     DAZFJL010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.215"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..233)
                     /locus_tag="ACHJ8C_004478"
     CDS             complement(<1..233)
                     /locus_tag="ACHJ8C_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO5554208.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYA"
     gene            complement(348..423)
                     /locus_tag="ACHJ8C_004479"
     tRNA            complement(348..423)
                     /locus_tag="ACHJ8C_004479"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(388..390),aa:Thr,seq:ggt)
     gene            complement(430..504)
                     /locus_tag="ACHJ8C_004480"
     tRNA            complement(430..504)
                     /locus_tag="ACHJ8C_004480"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(469..471),aa:Gly,seq:tcc)
     gene            complement(621..705)
                     /locus_tag="ACHJ8C_004481"
     tRNA            complement(621..705)
                     /locus_tag="ACHJ8C_004481"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(669..671),aa:Tyr,seq:gta)
     gene            complement(714..789)
                     /locus_tag="ACHJ8C_004482"
     tRNA            complement(714..789)
                     /locus_tag="ACHJ8C_004482"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(754..756),aa:Thr,seq:tgt)
     gene            809..964
                     /locus_tag="ACHJ8C_004483"
     CDS             809..964
                     /locus_tag="ACHJ8C_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554209.1"
                     /translation="METCEFMQLKNCIICFIGHILCDTMKKQPNGRQISTLFQHHCAQ
                     QCAPAKP"
     gene            1151..2101
                     /gene="coaA"
                     /locus_tag="ACHJ8C_004484"
     CDS             1151..2101
                     /gene="coaA"
                     /locus_tag="ACHJ8C_004484"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO5554210.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2130..3095)
                     /gene="birA"
                     /locus_tag="ACHJ8C_004485"
     CDS             complement(2130..3095)
                     /gene="birA"
                     /locus_tag="ACHJ8C_004485"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO5554211.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEKILSQLDDGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQHAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDRQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3092..4120)
                     /gene="murB"
                     /locus_tag="ACHJ8C_004486"
     CDS             complement(3092..4120)
                     /gene="murB"
                     /locus_tag="ACHJ8C_004486"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO5554212.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCIGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPSTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETANALLAQFPTAPHYPQVDGSVKLAAGWLIDQCQLKG
                     TQIGGAAVHRQQALVLINEHDAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1105 a         1052 c          950 g         1140 t
ORIGIN      
        1 gcgtagtgac gggtcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
       61 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcgc gagcagcacc gccgtaggtt
      121 ttagccagta cggtagtgat tgcagcggtc agcgttgttt taccatggtc aacgtggccg
      181 atagtaccga cgttaacgtg cggttttgta cgttcaaact tttctttaga catcgattgt
      241 ccctctaaga cacggataaa tcggtgatat caccacatca accaggcaac atgcccgact
      301 tgttgaatgc aataaacaga aggaaaaaac agggaggaga aaagaagtgg tgctgatagg
      361 cagattcgaa ctgccgacct cacccttacc aagggtgcgc tctaccaact gagctatatc
      421 agcacatctt ggagcgggca gcgggaatcg aacccgcatc atcagcttgg aaggctgagg
      481 taatagccat tatacgatgc ccgcatcctg gaactcggct acctgatttt cattctgcac
      541 tgaatatcga gagaagctct ctttattcga gccggtaagc gaacttatcg tctcgggcta
      601 cgccatcgcg tggccgaaat tggtggtggg ggaaggattc gaaccttcga agtctgtgac
      661 ggcagattta cagtctgctc cctttggccg ctcgggaacc ccaccggact tgatggtgcc
      721 gactaccgga atcgaactgg tgacctactg attacaagtc agttgctcta cctactgagc
      781 taagtcggca tcaagtagcg cgcattctat ggagacatgc gagttcatgc aactaaaaaa
      841 ttgcataatt tgttttattg gtcacatttt atgcgacacg atgaagaaac agccgaacgg
      901 aaggcagatt agcacacttt ttcaacatca ttgtgctcaa caatgtgctc ctgctaaacc
      961 ataattcttt ttatcagatg gaatatctgt cacattgctt ttcaacgata gcttcctggg
     1021 agagattttt tcttattatt cctccccatc tggtgttacc ctcctgccca ttaacccatt
     1081 caacagaact gtgacgcgcc atggcaaata tcgctttgcc gatagagcta tgaccgccag
     1141 aaacatgctt atgagtataa aagagcaaac gttaatgacg ccttacctac agtttgaccg
     1201 caaccagtgg gcagctctgc gtgattccgt acctatgacg ttatcggaag atgagatcgc
     1261 ccgtctcaaa ggtattaatg aagatctctc gttagaagaa gttgccgaga tctatttacc
     1321 tctgtcacgt ttgctgaact tctatataag ctcgaatctg cgccgtcagg cagttctgga
     1381 acagtttctt ggtaccaacg ggcaacgcat tccttacatt atcagtattg ctggtagtgt
     1441 cgcggtaggg aaaagtacaa ccgcccgtgt attgcaggcg ctattaagcc gttggccgga
     1501 acatcgtcgt gttgaactga tcactacaga tggcttcctt caccctaatc aggttctgaa
     1561 agaacgtggt ctgatgaaga agaaaggctt cccggaatcg tatgatatgc atcgcctggt
     1621 gaagtttgtt tccgatctca aatccggcgt gccaaacgtt acagcccctg tttactcgca
     1681 tcttatttat gatgtgatcc cggatggaga taaaactgtt gttcagcctg atattttaat
     1741 tcttgaaggg ttaaatgtct tacagagcgg gatggattat ccacacgatc cacatcatgt
     1801 atttgtttct gattttgtcg atttttcgat atatgttgat gcaccggaag acttacttca
     1861 gacgtggtat atcaaccgtt ttttgaaatt ccgcgaaggg gcttttacag acccggattc
     1921 ctattttcat aactacgcga aattaactaa agaagaagct attaagactg ccatgacatt
     1981 gtggaaagag atcaactggc tgaacttaaa gcaaaatatt ctacctactc gcgagcgtgc
     2041 cagtttaatc ctgactaaaa gtgctaatca tgcagtagaa gaggtcagac tacgcaaata
     2101 attttcaggg gagctaatgc tcccctttct tatttttctg cactacgcag ggatatttca
     2161 ccgcccatcc agggttttat tattccatcc tgctcaagta ataaagcacc ctgtctgtct
     2221 attccgcgtg aaatgccaaa tatttcttta tcaccaatga taagtttcac tgggcgatta
     2281 ataaaattat ccaacttttc ccagcgcgaa agataaggtg ccaatccttc ttgctcgaag
     2341 agttccagcg ccgcgcgtaa ttcacgtatc agcatggccg caagagtatt acgatcgaga
     2401 ttgatcccgg cttcctgcag cgtgatccac ccctgattaa cgacactctc ttcaacacga
     2461 cgcattgcca tgttgatccc tgcaccaatg actatttgcg ccgcatcgcc ggttttgcca
     2521 gtcagctcca caagaatgcc tgccagcttg cgatcctgca gatagaggtc attaggccat
     2581 ttaacacgaa ctttatctgc acccagcttg cgtaatactt ccgccatcac gataccgata
     2641 accagactta aaccaatcgc cgccgccggg ccctgttcca gacgccagaa catcgacaaa
     2701 tataagttcg cgccaaaagg cgaaaaccat ttccgaccac gacgaccacg gccagcatgc
     2761 tgatattctg caacacaggc atcgccagat ttaagctctc cgatgcggtc caggagatac
     2821 tgattcgtgg agtcaataac tggcagcacg gctacactgc cgtcatccag ctgactcaat
     2881 atcttttcgg cattaagtaa ctggattggc tcgggcaagc tgtatccttt acccggaaca
     2941 gtaaatacat caacgcccca atcacgcagt gtctgaatgt gtttattaat agccgcccgg
     3001 ctcattccca gcgtttcacc caactgctcg ccagagtgaa attcgccatt cgctaacagg
     3061 gcgatcaatt tcagtggcac ggtgttatcc ttcatgaaat tgtctccact gcgctcactt
     3121 cacctgatgc accaataaag cggacttcag gctcaagcca gacattaaat ttttcaccca
     3181 ctttttgtct tacatgatgc gccagctgta caacatcttc gctttttgca tcgtgctcat
     3241 ttatgagaac cagcgcctgt tgacggtgta ccgcagcccc accaatttgc gtccctttaa
     3301 gctggcactg atcaataagc caacctgcag ccagttttac tgaaccatcc acctgggggt
     3361 aatgtggggc tgttggaaat tgtgccaaca acgcgttagc agtttcggca gaaacaacag
     3421 gatttttgaa gaaactaccc gcattaccgt tcacttttgg atcagggagt ttggtggtgc
     3481 gcatatgaca caccgcatca aatacttgct gtggcgttac cgttgaagga tccaggcgag
     3541 ttaagtcacc atacgtcagt acaggttgcc actcttttgg cagacgcaga cctacggcta
     3601 caatggcgaa gcgatcctgg tattcatgtt taaaaatact gtctcgatag ccaaaacggc
     3661 actctttggc agttaagcgt acttgcttgc ctgtcgccag ttcaacacaa tcaacataag
     3721 cgcaaactcg ctgtaattct acgccataag caccaatatt ctggataggt gatgagccga
     3781 tacaaccagg aattaatgcc agattttcca gaccaggcat accttcctgc aaagtgtatt
     3841 ttaccaggcg atgccagttt tctccggctc ctacatgtaa ataccacgca tcaggttcat
     3901 catgaatttc gatacctttg atccggttga tgatcaccgt gccgcgatag tcctccagaa
     3961 aaagtacatt acttccttca cccagaataa gaacgggttg tccttctgcg gttgcatgct
     4021 gccaggcatt gagtaattgt tgttcgtctt cggcacatac aatgtgctga gcattatgat
     4081 caatgccaaa tgtgttccag ggttttaagg agtggttcat agctgctttc ctgatgcaaa
     4141 aacgaggcta gtttaccgta tctgtggggg gatggcttgt agatatgacg acaggaagag
     4201 tttatagaaa cgcaaaaagg cccagtcttc cgactgggcc tttcgct
//
LOCUS       DAZFJL010000214         4188 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZFJL010000214 DAZFJL010000000
VERSION     DAZFJL010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.216"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..183
                     /locus_tag="ACHJ8C_004487"
     CDS             <1..183
                     /locus_tag="ACHJ8C_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5554213.1"
                     /translation="AASTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEI
                     STADEGDGGQVVVIGR"
     gene            244..3639
                     /locus_tag="ACHJ8C_004488"
     CDS             244..3639
                     /locus_tag="ACHJ8C_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5554214.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDAEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDPEQFGSQQVSRNYHLRGRILQVPSNYDPEKRTYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWTDPDNGWETATELVEDSQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTAETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRIADIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGQITESHLGKELLEKVELTEDNASKLEEFSKEWKDAN
                     DKWNAMWGVKIEQTEDGRHYVAGLGLSMEDTEEGKLSQFLVAANRIAFIDPSNGNTRP
                     MFVGQGDQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGRLTAKNADISGSVNANSGT
                     LNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPF
                     DRQIVIPAVAFSGARHERENSDTYSSCRLIVKKNGAEIYNRTALDNTLIYTGVIDMPA
                     GSGVMTLEFSVSAWWVNGWYPTASISDLLVVVMKKATAGITIS"
     gene            3707..>4188
                     /locus_tag="ACHJ8C_004489"
     CDS             3707..>4188
                     /locus_tag="ACHJ8C_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5554215.1"
                     /translation="MRKLCAVILSTVVWLVAAGTPASAAEHQSTLSAGYLQTHTDMPG
                     NDDLKGINVKYRYEFTDTLGLVTSFSYANAKDEQKTHYSDTRWHEDSVRNRWFSMMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     G"
BASE COUNT          989 a         1033 c         1305 g          861 t
ORIGIN      
        1 gcagccagta cagataacgg caaacagaac acctattttt cctcactgga taacatggtt
       61 gctcagggca atgttctgcc cgttctgtac ggtgaaatgc gtgtggggtc gcgggtggtt
      121 tctcaggaga tcagcacggc agacgaaggt gacggtggtc aggttgtggt gattggtcgc
      181 tgatgcaaaa tgttttatgt gaaaccgcct gcgggcggtt ttatcgttta tggagcatga
      241 cgaatgggta aaggcagcag taaggggcat accccgcgcg aagcgaagga caacctgaaa
      301 tccacgcagt tgctgagtgt gattgatgcc atcagtgaag ggccggttga aggtccggtg
      361 gatggattaa aaagcgtgct gctgaacagt acgccggtgc tggacgctga ggggaatacc
      421 aacatctccg gtgtcacggt ggtgttccgg gcaggtgagc aggagcagac accgccggag
      481 gggtttgaat cctccggctc cgagacggtg ctgggtacgg aagtgaaata cgacacgcct
      541 atcacccgga ccatcacgtc ggcaaacatc gaccgtctgc gctttacctt cggtgtgcag
      601 gcactggtgg aaaccacctc aaagggtgac aggaatccgt cggaagtccg cctgctggtt
      661 cagatacagc gtaacggtgg ctgggtgacg gaaaaagaca tcaccattaa gggcaaaacc
      721 acgtcgcagt atctggcctc ggtggtggtg gataacctgc cgccgcgccc gttcaacatc
      781 cggatgcgca ggatgacgcc ggacagcacc acagaccagc tgcagaacaa aacgctctgg
      841 tcgtcataca ccgaaatcat cgatgtgaaa cagtgctacc cgaacacggc actggttggc
      901 gtgcaggtgg acccggagca gtttggcagt cagcaggtga gccgtaatta tcatcttcgc
      961 gggcgcattc tgcaggtgcc gtcaaactat gatccggaaa aacgcactta cagcggcatc
     1021 tgggacggaa cgttaaaacc ggcatacagc aacaacatgg catggtgtct gtgggatatg
     1081 ctgacccacc cgcgctacgg catggggaaa cgtcttggtg cggcggatgt ggataaatgg
     1141 gcgctgtatg tcatcggcca gtactgcgat cagtcagtgc cggacggttt tggcggcacg
     1201 gagccgcgca tcacctgtaa tgcgtacctg accacacagc gcaaggcgtg ggatgtgctc
     1261 agtgatttct gctctgcgat gcgctgtatg ccggtatgga acgggcagac gctgacgttc
     1321 gtgcaggacc gaccgtcgga taaggtgtgg acctataacc gcagtaatgt ggtgatgccg
     1381 gatgatggcg cgccgttccg ctacagcttc agcgccctga aagaccgcca taatgccgtt
     1441 gaagtgaact ggactgaccc ggataacggc tgggagacgg caacagagct tgtggaggac
     1501 tcgcaggcca ttgcccgtta cggtcgtaac gtcacgaaga tggatgcctt tggctgtacc
     1561 agccgggggc aggcacaccg cgccgggctg tggctgatta aaacggagct gctggaaacg
     1621 cagaccgtgg atttcagtgt gggcgcagaa gggcttcgcc atgtgccggg cgatgttatt
     1681 gaaatctgcg atgatgacta tgccggtatc agcaccggtg gtcgtgtgct ggcggtgaac
     1741 agccagaccc ggacgctgac gctcgaccgt gaaatcacgc tgccatcctc cggtaccacg
     1801 ctgataagcc tggttgacgg aagtggcaat ccggtcagcg tggaggttca gtccgtcacc
     1861 gacggcgtga aggtgaaagt gagccgtgtt cctgacggcg ttgctgaata cagcgtatgg
     1921 gggctgaagc tgccgacgct gcgccagcgc ctgttccgct gcgtgagtat ccgtgagaac
     1981 gatgacggca cgtatgccat cactgccgtg cagcatgtac cggaaaaaga agccatcgtg
     2041 gataacgggg cgcactttga cggcgaccag agcggcacgg tgaatggtgt cacgccgcca
     2101 gcagtgcagc acctgactgc cgaagtcacc gcagacagcg gggaatatca ggtgctggcc
     2161 cgctgggata cgccgaaggt ggtgaagggc gtgagtttca tgcttcgcct gaccgtggca
     2221 gcggatgacg gcagtgagcg gctggtcagc acagcccgga cggcggaaac cacataccgc
     2281 ttcacacaac tggcgctggg gaactacagg ctgacagtcc gggcagtaaa tgcgtgggga
     2341 cagcagggag acccggcgtc ggtatcgttc cggattgccg ccccggcagc accgtcgagg
     2401 attgagctga cgccgggcta ttttcagata accgccacgc cgcatcttgc ggtttatgac
     2461 ccgacggtac agtttgagtt ctggttctcg gaaacgcgga ttgcggatat caggcaggtt
     2521 gaaaccacag cccgctatct tggcacgggg ctgtactgga tagccgccag tatcaatatc
     2581 aaaccgggcc atgattatta cttttatatc cgcagcgtga ataccgttgg taaatcggca
     2641 tttgtggagg ccgtcggtcg ggcgagcgat gatgcggaag gttatctgga ttttttcaaa
     2701 ggccagataa ccgaatccca tctcggcaag gagctgctgg aaaaagtcga gctgacggag
     2761 gataacgcca gcaaactgga ggagttttcg aaagagtgga aagacgccaa cgataaatgg
     2821 aatgccatgt ggggcgtcaa aattgagcag accgaagacg gcaggcatta tgtcgcgggg
     2881 cttggcctca gcatggagga tacggaggaa ggcaaactga gccagttcct ggttgccgct
     2941 aaccgtatcg cgtttattga tccgtctaat ggcaacacaa gaccaatgtt tgttggtcag
     3001 ggcgatcaga tattcatgaa cgacgtgttc ctgaaacgcc tgacggctcc gaccattacc
     3061 agcggcggta atcctccggc attttccctg acaccggacg ggcggctgac ggcgaaaaat
     3121 gccgatatca gcggtagcgt gaatgcgaac tccgggacgc tcaacaacgt cacgattaac
     3181 gagaactgtc gggttctggg aaaactgtcc gcgaaccaga ttgaaggcga tctcgttaaa
     3241 acagtgggca aagctttccc ccgggactcc cgtgcaccgg agcggtggcc atcagggaca
     3301 attaccgtca gggtttatga cgatcagccg tttgaccggc aaattgttat tccagcggtg
     3361 gctttcagcg gtgccagaca tgaacgggag aatagcgata cttattcgtc atgccgcctg
     3421 atagtgaaga aaaacggggc tgaaatttat aaccgaacgg ctctggataa tacgctgata
     3481 tacacgggtg ttattgatat gcctgcaggc agtggcgtaa tgacactgga gttttctgta
     3541 tcagcatggt gggtaaatgg ctggtatccc acagcaagta tcagcgattt gctggttgtt
     3601 gtgatgaaga aagccactgc aggcatcacg attagctgaa ttttataacc cagatacggg
     3661 cgccagaaat ggcgcctttt ttattgcaga aaagcgagag gtaattatgc gtaaattatg
     3721 tgctgttatt ctgtccacag tagtctggct ggtcgccgct ggtacgccag cgagcgcagc
     3781 agagcatcag tccacactaa gcgccgggta tcttcagacc catactgata tgcccggcaa
     3841 cgatgacctg aagggcatta acgtgaaata ccgttatgaa tttacggaca cgctggggct
     3901 ggtgacgtca ttcagttatg ccaatgccaa agatgagcaa aaaacgcatt acagcgatac
     3961 ccgctggcat gaagattcag tgcgtaaccg ctggttcagc atgatggcgg ggccgtctgt
     4021 acgcgtgaat gaatggttca gtgcttatgc gatggcaggt gtggcttaca gccgtgtgtc
     4081 gacgttctcc ggggattatc tccgcgtaac tgacaacaag gggaaaacgc atgatgtgct
     4141 gaccggaagt gatgacggtc gccacagcaa cacgtctctg gcgtgggg
//
LOCUS       DAZFJL010000215         4170 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFJL010000215 DAZFJL010000000
VERSION     DAZFJL010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.217"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            1..298
                     /locus_tag="ACHJ8C_004490"
                     /pseudo
     CDS             1..298
                     /locus_tag="ACHJ8C_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            539..1504
                     /gene="lipA"
                     /locus_tag="ACHJ8C_004491"
     CDS             539..1504
                     /gene="lipA"
                     /locus_tag="ACHJ8C_004491"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO5554216.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(1604..1807)
                     /gene="tatE"
                     /locus_tag="ACHJ8C_004492"
     CDS             complement(1604..1807)
                     /gene="tatE"
                     /locus_tag="ACHJ8C_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO5554217.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(1936..2724)
                     /locus_tag="ACHJ8C_004493"
     CDS             complement(1936..2724)
                     /locus_tag="ACHJ8C_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO5554218.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENEVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLDRLRRESKRNMMTTILTIHVLSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTSYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            2817..3200
                     /gene="crcB"
                     /locus_tag="ACHJ8C_004494"
     CDS             2817..3200
                     /gene="crcB"
                     /locus_tag="ACHJ8C_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO5554219.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTTH"
     gene            complement(3254..3463)
                     /gene="cspE"
                     /locus_tag="ACHJ8C_004495"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3254..3463)
                     /gene="cspE"
                     /locus_tag="ACHJ8C_004495"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO5554220.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(3638..4141)
                     /gene="pagP"
                     /locus_tag="ACHJ8C_004496"
     CDS             complement(3638..4141)
                     /gene="pagP"
                     /locus_tag="ACHJ8C_004496"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO5554221.1"
                     /translation="MEDASADTPQWLSTFKENVAETWQQPQHYDLYIPAITWHARFAY
                     DKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPL
                     ADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGPATFQMTYIPGTYNNGNVYF
                     AWMRFQF"
BASE COUNT         1086 a         1067 c          957 g         1060 t
ORIGIN      
        1 accctgttta agtagaaaat cgtcagaagg taaaccacaa atatttacaa ccttacgaaa
       61 tattgatctt actcttctga ctattttgaa gttgcatatg tccataaagg aatcgttaat
      121 gcagcgaaag tgcttaatct gccccccctc ggtcggagcc ctggtccttc ctgtcatcta
      181 tcgggcgatt aaaactcact atccacagct tttactgtgc aacccgccca tcggcggcgc
      241 ggaaaaccaa ctcagtcagt ttcaaaccga tctcatcacc gataacatgt tttgcacagg
      301 accatagatg ttatccgtaa tgcattttga aaaagtattg ttacagccag atcacagcgc
      361 agaacgataa aatggcacaa tccaacaaca attttacatt ttcgcgaccg ctttggctgc
      421 tttcaggtcc gtttcaatga tatactgcca gtcgttaatt caaaaatagt tgataattac
      481 aacaatctat tgaattgaaa cgctttcctt cgtaattcgc aactggaaca cgcacgctat
      541 gagtaaaccc attgtgatgg aacgcggtgt taaataccgc gatgccgata agatggccct
      601 tatcccggtt aaaaacgtgg caacagagcg cgaagccctg ctgcgcaagc cggaatggat
      661 gaaaatcaag cttccagcgg actctacacg tatccagggc atcaaagccg caatgcgcaa
      721 aaatggcctg cattctgtct gcgaggaagc ctcctgccct aacctggcgg aatgctttaa
      781 ccacggcaca gcaacgttta tgatcctcgg cgctatttgt acccgccgtt gcccgttctg
      841 tgacgttgcc cacggtcgcc cggtagctcc tgatgccaat gaaccagtga aactggcgca
      901 gacaattgcc gatatggcgc tgcgttatgt ggttatcacc tccgttgacc gtgatgatct
      961 gcgcgatggc ggtgcccagc actttgcgga ttgcattact gccattcggg aaaaaagccc
     1021 gcaaatcaaa attgaaactc tggtgccgga tttccgcggt cgtatggatc gtgctctgga
     1081 tattctgact gcaacgccac cagatgtgtt caaccataac ctggaaaacg taccgcgtat
     1141 ttaccgtcag gtacggcctg gcgcagatta caactggtcg cttaagctgc tggaacgctt
     1201 taaagaagcg catccggaaa tcccgaccaa gtctggtctg atggtgggac tgggtgaaac
     1261 caatgaagaa attattgagg taatgcgcga cctgcgccgt catggtgtga cgatgctaac
     1321 gctggggcaa tatttgcagc caagccgcca tcacctgccg gttcaacgtt acgttagccc
     1381 ggatgagttc gacgaaatga aagccgaagc gctggcgatg ggctttaccc atgctgcatg
     1441 cggtccgttt gtccgctctt cttaccacgc cgatttgcag gcgaaaggga tggaagttaa
     1501 gtaatcctgt aataattact tacatctgtc tgataaaaaa cccgcttttt gaagcgggtt
     1561 tttgtatcga acaagatatt gagggagcgt cctgctcgcc acgtcactct ttatgagaga
     1621 gcttttcagc ctgaagatca acgtctgcgc cttttttcgc cgcagcatcg tcatcattca
     1681 tcgccttctt gaacccttta atggccgctc caaggtctcc gcccagcgta cgtaacttct
     1741 tagtcccaaa cagcagaacg accagcgccg caactaccag cagtttggta atactaatct
     1801 cacccataga taccttcttg acatataacc ggcaacagaa cgcgccttaa aaaccagaga
     1861 atattaacgg tcattcgacg cgcgcacaca atagcaatct gtaacaaggt gaatcaagcg
     1921 ccgagtaaaa aaacatcata ataattgcgg cggcgcaaag cgacggttgt ttaagacggg
     1981 cagttgcgcg cgtacctgac gcacacgttc gggcgtcact tccgccataa tgagtgcagg
     2041 catttctgac gctgccgcaa tggtgacgcc aaagggatct ataatccggc tttgcccgat
     2101 atttttgttc ccgcactccc ccgccgccac catataagag gtggtatcca gcgcacgagc
     2161 ggcaagcaac gttgaccagt gatgctcttt gagcggccca cgaacccagg cggcaggaag
     2221 taccaggatt tcagctccct gtaatgcctg tgccagcgcc agctctggaa agcgtaagtc
     2281 ataacaggtc atcagaccga ccttcatccc ttccacctcc agtaacggag ctatttcatt
     2341 accggcatca acatggcgtg attcctgaat ggcaaatgca tcatagagat gcagtttggc
     2401 ataacgggcg acgatgtttc ctgcctgtag cgccaccagc atattccatg cgcgccccgg
     2461 cgttgaaaga acatgaatcg tcagaattgt cgtcatcatg ttacgtttac tttctcgccg
     2521 taaacggtcg aggaattcgc cttccagcag ctgtgctgat ttaaccgaaa gatccgcatc
     2581 atgatcatca cgcgccagta atgcttccgg caggacaaac agcgaaacct cgttttccgc
     2641 cgcctgcgcc atcaacgagg cacaaatctc agcgttcttt tcccacacag atgtaacagc
     2701 aaactgtcct gcggcaacca gcattgccac ctcctgtcag agtcgtaaaa ttcgccaacg
     2761 gcgattattc ggcgttacac ttatcactca tacaaatcaa atagcaggat tttgcagtgt
     2821 tacaacttct tttagcagtt tttattggcg gtggtacggg aagcgtggcg agatggctgt
     2881 taagtatgcg atttaacccg ctgcatcagg cgattccgtt ggggacgctg gcagcaaacc
     2941 tgattggggc attcatcata ggaatgggat tcgcctggtt cagcaggatg acgaacattg
     3001 atccagtgtg gaaagtatta atcaccaccg gattttgtgg cggtctaaca accttctcaa
     3061 cattttcggc agaagtggtg tttttgttac aagagggccg ctttggctgg gcgttactga
     3121 acgttttcgt caatcttttg gggtcttttg ccatgaccgc actggctttc tggctgtttt
     3181 cggcctcaac cacacactaa agaccaaaaa aaacccgctg attaagcggg ttttgaattc
     3241 ttgctgacgt atcttacaga gcgattacgt ttgcagcaga agggcctttg gcaccgttag
     3301 tgatttcgaa ctctacgcgc tgaccttcag caagagtttt aaaaccatta gtctggattg
     3361 cagagaagtg tacgaacacg tctttgctgc cgtcttccgg agtaatgaaa ccgaatcctt
     3421 tggactcatt aaaccactta acgttacctt taatcttaga catcaaaatt acctttacat
     3481 gaaaaataga cacaaatgct gtgtcggtta ccagtacacc aattgtggta cgctttgtcc
     3541 agtgcaactt tactaaaaag tgataaaagt cgcgttattt ttgatgttaa ttattttagt
     3601 ggttgatttt aaagaattta ctaaaacttc atttgtctca aaactgaaag cgcatccagg
     3661 caaagtacac attgccattg ttgtaggtac ccggaatgta ggtcatctga aacgtcgcgg
     3721 ggccataccc cacagaggcc aatggcagta gaaccggaag agggatgtaa ttccagttat
     3781 cgcgcgccgt tacgccagcg gtaaatccca ggcctaaatg aaaattttca tccgccagcg
     3841 gtcgccaggt actttcccat ccgtatccgg caatcggttc ccatttgttc cacgagtcct
     3901 taaatgccat ggcatacagg ccatgccagt ttcctttttc atcccaacgc gactgaccaa
     3961 aaccgccgcc ccacggtcgc tcgttatagc gatcggtttt ttctttgtca taggcaaaac
     4021 gcgcatgcca ggtgatcgcg ggtatgtaca agtcataatg ttgtggctgt tgccatgttt
     4081 ctgcaacatt ctctttaaat gtcgacagcc attgaggtgt gtcagcagaa gcgtcttcaa
     4141 tgccgactaa ttgagtaaaa acaaagagaa
//
LOCUS       DAZFJL010000216         4133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZFJL010000216 DAZFJL010000000
VERSION     DAZFJL010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.218"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            164..2344
                     /gene="katG"
                     /locus_tag="ACHJ8C_004497"
     CDS             164..2344
                     /gene="katG"
                     /locus_tag="ACHJ8C_004497"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO5554222.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            2438..3343
                     /gene="yijE"
                     /locus_tag="ACHJ8C_004498"
     CDS             2438..3343
                     /gene="yijE"
                     /locus_tag="ACHJ8C_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO5554223.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(3370..3987)
                     /locus_tag="ACHJ8C_004499"
     CDS             complement(3370..3987)
                     /locus_tag="ACHJ8C_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO5554224.1"
                     /translation="MKASLALFSLLTVFTSYSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     AYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFAK"
BASE COUNT          919 a         1050 c         1168 g          996 t
ORIGIN      
        1 aatcggacat aatcaaaaag tcttaattac gatcaatttg atctacatct ctttaaccaa
       61 caatatgtaa gatctcaact atcgcatccg tggattaatt caattataac ttctctctaa
      121 cgctgtgtat cgtaacggta acactgtaga ggggagcaca ttgatgagca cgtcagacga
      181 tatccataac acgacagcca ccggcaaatg cccgttccat cagggcggtc acgaccagag
      241 cgcaggtggg ggcacaacca cgcgcgactg gtggccaaat caactccgtg ttgacctgtt
      301 aaaccaacat tctaatcgtt ctaacccgct gggtgaggac tttgactacc gcaaagaatt
      361 cagcaaatta gattactacg gcctgaaaaa agatctgaaa gccctgctga cagaatctca
      421 accgtggtgg ccagccgact ggggcagcta cgccggtctg tttattcgta tggcctggca
      481 cggcgcgggt acttaccgtt caatcgacgg tcgcggcggc gcgggtcgtg gtcagcaacg
      541 ttttgcaccg ctgaactcct ggccggataa cgtaagcctc gataaagcgc gtcgcctgtt
      601 gtggccaatc aaacagaaat atggccagaa aatctcctgg gccgacctgt ttatcctcgc
      661 gggtaacgtg gcgctggaaa actccggctt ccgtaccttc ggttttggtg ccggtcgtga
      721 agacgtctgg gaaccggatc tggatgttaa ctggggtgat gaaaaagcct ggctgactca
      781 ccgtcatccg gaagcgctgg cgaaagcacc gctgggtgca accgagatgg gcctgattta
      841 cgttaacccg gaaggcccgg atcacagcgg cgaaccactt tcggcggcag cagctatccg
      901 cgcgaccttc ggcaacatgg gcatgaatga cgaagaaacc gtggcgctga ttgcgggtgg
      961 tcatacgctg ggtaaaaccc acggtgctgg tccgacatca aacgtaggtc ctgatccaga
     1021 agctgcaccg attgaagaac aaggtttagg ctgggcgagc acttacggca gcggcgttgg
     1081 cgcagatgcc attacctctg gtctggaagt ggtctggacc cagacgccga cccagtggag
     1141 caactatttc ttcgagaacc tgttcaagta tgagtgggta cagacccgca gcccggctgg
     1201 tgcaatccag tttgaagcgg tagacgcacc ggaaattatc ccggatccgt ttgatccgtc
     1261 gaagaaacgt aaaccgacga tgctggtgac cgacctgacg ctgcgttttg atcctgagtt
     1321 cgagaagatc tctcgtcgtt tcctcaacga tccgcaggcg ttcaacgaag cctttgctcg
     1381 tgcctggttc aaactgacgc acagggatat ggggccgaaa tctcgttaca tcgggccgga
     1441 agtaccgaaa gaagatctga tctggcaaga tccgctgccg cagccgatct acaacccgac
     1501 cgagcaggac attatcgatc tgaaattcgc aattgcggat tctggtctgt ctgttagtga
     1561 gctggtatcg gtggcctggg cgtctgcttc taccttccgt ggtggcgaca aacgtggtgg
     1621 tgccaacggt gcgcgtctgg cattaatgcc gcagcgtgac tgggatgtga acgccgcagc
     1681 cgttcgtgct ctgccagttc tggagaaaat tcagaaagag tctggcaaag cttcgctggc
     1741 ggatatcatc gtgctggcag gtgtggttgg cgttgagaaa gccgcaagcg ccgcaggttt
     1801 gagcattcat gtaccgtttg cgccgggccg cgttgatgcg cgtcaggatc agactgacat
     1861 tgagatgttc gaactgctgg agccaattgc tgacggtttc cgtaactatc gcgctcgtct
     1921 ggacgtttcc accaccgagt cactgttgat tgataaagca cagcaactga cgctgaccgc
     1981 gccggaaatg actgcgctgg tgggcgggat gcgcgtactg ggtgccaact tcgatggcag
     2041 caaaaacggt gtcttcactg accgcgttgg tgtattgagc aatgacttct tcgtgaactt
     2101 gctggatatg cgttacgagt ggaaagcgac cgacgaatcg aaagaactgt tcgaaggccg
     2161 tgaccgcgaa actggcgaag tgaaatacac agccagccgt gcggatctgg tctttggttc
     2221 taactctgtc ctgcgtgcgg tggcggaagt ttacgccagc agcgatgccc acgagaagtt
     2281 tgttaaagac ttcgtggcag cgtgggtgaa agtgatgaac ctcgaccgtt tcgacctact
     2341 gtaatctgac cttcttcagc gactgccttt catgcagtcg ctgaagtttc tttaccggcg
     2401 tatagtgtcc acaggaaaac tacacactgg atctctcatg tctgccgcag gaaagagcaa
     2461 cccactggca atcagtggcc tggttgtgct cacacttatc tggagttata gctggatttt
     2521 catgaagcaa gtcaccagct acatcggtgc cttcgacttc accgccttac gctgcatttt
     2581 cggcgctctc gttttattca tcgtcctttt attacgtggt cgcggaatgc gcccgacgcc
     2641 gtttaaatac accttagcca ttgccctgtt acaaacctgc gggatggttg gcctggcaca
     2701 gtgggcgctg gtcagcggcg gagcggggaa ggtggcaatc ctgagctata ccatgccgtt
     2761 ctgggtggta attttcgccg cgttgtttct cggtgaacgc ctgcgacgtg ggcaatattt
     2821 cgcgatactg attgccgctt tcggcttatt tttggtgttg cagccgtggc aactcgattt
     2881 ctcttcgatg aaaagtgcca tgctggcaat cctctccggt gtgagttggg gggcgagcgc
     2941 gattgttgct aaacgtctgt atgcccgcca tccgcgcgtg gatttattgt cgctaacatc
     3001 ctggcagatg ctgtatgcgg cgctggtgat gagcgtggtc gctttactgg tgccgcaacg
     3061 tgaaattgac tggcaaccca ccgtgttctg ggcgctggcc tacagtgcga ttctggcgac
     3121 agcgctggcg tggagcttat ggttgtttgt attgaaaaac ttgcctgcca gtattgccag
     3181 cttaagcaca ctggccgttc ccgtttgcgg cgtactcttt tcctggtggc tgctcggtga
     3241 gaatccgggg gcagttgaag gtagcggtat tgtgctgatt gtactggcac tggcgctggt
     3301 gagccgtaag aaaaaagaag ccgtcagtgt aaaaaggatc tgaatttttt cttcatgtgg
     3361 ggagatctct tatttagcaa aataccgata gtgccccacc atccgccagc taaacagtac
     3421 atcttcttcc tgcgcgcctg cgccaatgtt atgtatcacc agcggcgtac cgtcgcgggc
     3481 gaagccatct gacaccaccc caatatgtgc cagcccgtta tctaatcgcc aggagacaat
     3541 atcgcccgct tgatagtcac tggggttctt gctggtgggg cgtgttttat cgtggcgggt
     3601 aaaccaggtt tccagattag gcacccgacg gtgatcgatg ttgctgtccg ggcgctttaa
     3661 ctgccatttt tgcgggtacg cagcaaaatt cttcgccata tcttcgtgca ccagtttctg
     3721 caaatcgacc ttctggctgc gcaatgcacg gatcaccaca tcggaacata cgccgcgttc
     3781 ttgcgggaca tcaccgccag gataggtaag ctgcacatac gccggatcgt aaaatagcgt
     3841 gctgccgatt tgctgtctgg cgccgtctgc gatggcaaga ttggtgttag cctggatttg
     3901 taccacggtt ggtggaacgg cgggagattt taaggagtaa ctggtaaata ccgtcagaag
     3961 actgaataac gccagtgaag ctttcatctg tttcgtccgt gaaaataaga gtcacagatt
     4021 cgaccttccg ggctaacgat tgttctgtgg agaaaatgtc cagaagcgga taaaaaaaca
     4081 cccgaacaag gtggaagttt cagattactg gcacagcgtg ccggatgcgg cgt
//
LOCUS       DAZFJL010000217         4130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFJL010000217 DAZFJL010000000
VERSION     DAZFJL010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.219"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(218..964)
                     /gene="cutC"
                     /locus_tag="ACHJ8C_004500"
     CDS             complement(218..964)
                     /gene="cutC"
                     /locus_tag="ACHJ8C_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO5554225.1"
                     /translation="MALLEICCYSMECALTVQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGITRVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(978..1544)
                     /locus_tag="ACHJ8C_004501"
     CDS             complement(978..1544)
                     /locus_tag="ACHJ8C_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO5554226.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1760..3493
                     /gene="argS"
                     /locus_tag="ACHJ8C_004502"
     CDS             1760..3493
                     /gene="argS"
                     /locus_tag="ACHJ8C_004502"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO5554227.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTYLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3546..3938)
                     /gene="flhe"
                     /locus_tag="ACHJ8C_004503"
     CDS             complement(3546..3938)
                     /gene="flhe"
                     /locus_tag="ACHJ8C_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO5554228.1"
                     /translation="MRALLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIPAAEPL
                     RFIWEVPGGGRLIPPLKIQRNEVIVNYR"
     gene            complement(3938..>4130)
                     /locus_tag="ACHJ8C_004504"
     CDS             complement(3938..>4130)
                     /locus_tag="ACHJ8C_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554229.1"
                     /translation="AQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLS
                     NLELSDNRHIRMTATIGGK"
BASE COUNT         1037 a         1095 c         1045 g          953 t
ORIGIN      
        1 atatatattt gtcagttttg gcaatttaaa tcacataatg aatgtgaata tataaaatat
       61 ttttagcaca acgctatgcg cactgtcacc cgctcaacag atcacggcaa tcgttcgttt
      121 tttatactgc tcagggataa aaaaaggccc ctgttgaaat tgcaggggcc tggtacgagc
      181 aagcatcata ttgggcgaca tgatgcaacg gtaaaaatca tttggcctga tggcgttcaa
      241 tgattccttt catttcagca accgccgccc cgtctacgac ataacgcgaa tactcgtccg
      301 cgtgtgcatc tgatgacatg gacaatcctt gattacgata acgcatcggt gaggcttgcc
      361 acgctcccgc ggagctatgg acttccagca ctccggcatc gaggaagtgg tgcaggtttt
      421 ccgcacggac tcctgctccg gccataatga ttggagcatc acgatgggca ataagttcca
      481 taatttttga taaaccttgc agtgcgtctg atttttgccc tgatgtcagt acccgggtaa
      541 tgcccaattc cgcaagatta ttgagtgtat ttaaagggtt agcgcacata tcgaaggcgc
      601 gatgaaaagt cactgccagc ggaccggcag cagccattat tttttccatt cgtggcatat
      661 cgacattccc gtcaacatcg agaacgcccg tcaccagtcc aggaaaacct aattcgcgga
      721 ctgtgcgcac atcttcaaga atggcggcaa actcaccgtc gctgtagcaa aaatcaccac
      781 cgcgtgggcg gataatcgga tgcactggga tcgtcacccg ctggcgcacg gatttcagta
      841 cccccaacga cggcgttaag cccccctctt ttggagctgc gcataattca actctgtcag
      901 cgccgttttg ctgcaccgtt agcgcgcatt ccatgctgta acagcaaatt tccagtaatg
      961 ccatttttgc tccttagtta cgccgactgc tcgctggcaa cgatctcttc aatggaccac
     1021 ggatgaaact taatggttgt tttaccatcg gtgaccgcca gcgtcggatt cggtaatcgt
     1081 tcatgctcac cttttggcga actggtttta actgagatcc ccggcaggct gagtccttca
     1141 tcagaaagca atgccagcgc acgggcattc agcgtttctg gatcacccgg cagaacgatt
     1201 tcaatatgtt cccaaccttc gtgcgggtaa cgtttttccc cgggccacgg tagctccaca
     1261 atggaaaact gccagtgcgc aacctgtacc ggttcatgca atttaaacag acaaatcggt
     1321 ctgccattga tcatattttc tgacaaaagc tcgccgcact gttcaaaccc gcgacgccag
     1381 cgttcagcgg tgacgttttg atggcaacgc aaggaaatgt gatcggcagt cagcggagtg
     1441 atatccaacc ccagacggcg ggaaagttca tctaatgcgt gggtaaatcg cggtaaatcc
     1501 gatgcaatat cctgcagctc gtcgatagat tgccagttcg ccataatcac tcttcgtctt
     1561 tcattaaaag cgttaattta ccctgttgcc ctgcgccaac caaccgctga tttcacgccg
     1621 cttctgatgc aatagtgaaa acggcaatac gccacgcgca cgttgctgac gaaaacagcc
     1681 atttgcagta tactcccgcc ctaatttctt taactggtgc gggcaatttt tgctcgcttc
     1741 atcaatgtaa ggtattccgg tgaatattca ggctcttctc tcagaaaaag tccgtcaggc
     1801 catgattgcg gcaggcgcgc ctgcggattg cgaaccgcag gttcgtcagt cagcaaaagt
     1861 tcagttcggc gactatcagg ctaacggcat gatggcagtt gctaaaaaac tgggtatggc
     1921 accgcgacaa ttagcagagc aggtgctgac ttatctggat cttaacggta tcgccagcaa
     1981 agttgagatc gccggtccag gctttatcaa cattttcctt gatccggcat tcctggctga
     2041 acatgttcag caggcgctgg cgtccgatcg tctcggtgtt gctacgccag aaaaacaaac
     2101 cattgtggtt gactactctg cgccaaacgt ggcgaaagag atgcatgtcg gtcacctgcg
     2161 ctctaccatt attggtgacg cagcagttcg tactctggag ttcctcggtc acaaagtgat
     2221 tcgcgcaaac cacgtcggcg actggggcac tcagttcggt atgctgattg cgtggctgga
     2281 aaagcagcag caggaaaacg ccggtgaaat ggagctggct gaccttgaag gtttctaccg
     2341 cgatgcgaaa aagcattacg acgaagatga agagtttgcc gagcgtgcac gtaactacgt
     2401 ggtaaaactg caaagcggtg acgaatattt ccgcgagatg tggcgcaaac tggtcgacat
     2461 caccatgacg cagaaccaga tcacctacga tcgtctgaac gtgacgctga cccgtgatga
     2521 cgtgatgggt gaaagcctct acaacccaat gctgccgggt atcgtggcgg acctgaaagc
     2581 caaaggtctg gcagtagaaa gtgaaggcgc gaccgttgta ttccttgatg agttcaaaaa
     2641 caaggaaggc gaaccgatgg gcgtgatcat tcagaagaaa gatggtggct atctctacac
     2701 caccactgat atcgcctgtg cgaaatatcg ttatgaaaca ctgcatgccg atcgcgtgct
     2761 gtattacatc gactcccgtc agcatcaaca cctgatgcag gcgtgggcta tcgtccgcaa
     2821 agcaggctat gtaccggaat ccgtaccgct ggaacaccac atgttcggca tgatgctggg
     2881 taaagacggc aaaccgttca aaacccgcgc gggtggtaca gtgaaactgg cagacctgct
     2941 ggatgaagcc ctggagcgtg cacgccgtct ggtggcagaa aagaacccgg atatgccagc
     3001 cgacgagctg gaaaaactgg ctaacgcggt tggtattggt gcggtgaaat atgcggatct
     3061 ctccaaaaac cgcaccacgg actacatctt cgactgggac aacatgctgg cgtttgaggg
     3121 taataccgcg ccatacatgc agtatgctta cacgcgtgta ttatccgtgt tccgtaaagc
     3181 agaaattgac gaagagcaac tggctgcagc tccggtaatc atccgtgaag atcgtgaagc
     3241 gcaactggca gctcgcctgc tgcagtttga agaaaccctc actgtggttg cccgtgaagg
     3301 cacgccgcat gtgatgtgtg cttacctgta cgatctggcc ggtctgttct ctggcttcta
     3361 cgagcactgc ccgatcctca gcgcagaaaa cgaagaagtg cgtaacagcc gtctgaaact
     3421 ggcacaactg acggcgaaga cgctgaagct gggtctggat acgctgggta ttgagacagt
     3481 agagcgtatg taatcgcgtt atacggcaac agccgatgtc attgcagaca tcggcttttt
     3541 tatcattaac gataatttac aatcacttca ttccgttgaa tctttagcgg cggaattaac
     3601 cgcccaccgc caggcacttc ccagataaag cgcaacggtt ctgccgccgg tataccggaa
     3661 aaggccacgg tggttccact ctgcccctct aattcgacac aacgagattg tgaacacaag
     3721 cgaacccgca gtcctgcagg tgtcggaccg ataagctgat aacgccacgc caccagcgtc
     3781 atcaatcctg aagcaggttg tcgcgtagaa agaggcgctg acgacatcga ttcaccgcga
     3841 tgatttagcg taacgcccac gctactcgcc tgccacatcc cctccccggc ggcttgcacc
     3901 agcagcggaa acaataatat tgctaataag gctctcatta tttgccgcca atcgtcgccg
     3961 tcatacggat atgtcggtta tctgatagtt ccagattcga cagcaccact aactgcggca
     4021 agctgcggcg caggaagcga gacaataatg gtcgcagcgc gtggttcacc aacagtaccg
     4081 gcggcgcacc caacatctcc tgacgggata gcgcttcctg agtttgcgcc
//
LOCUS       DAZFJL010000218         3921 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFJL010000218 DAZFJL010000000
VERSION     DAZFJL010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.220"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..1023
                     /locus_tag="ACHJ8C_004505"
     CDS             <1..1023
                     /locus_tag="ACHJ8C_004505"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO5554230.1"
                     /translation="MEFGIWGEPEMINPDSDLFRLHPDWILSMPGYSQPTGRYQYVLN
                     LNIPEAFAYIYERFLWLLGEHPVDYVKWDMNRELVQAGHEGHAAADAQTRQFYRLLDL
                     LRERFPHVEFESCASGGGRIDFEVLKRTHRFWVSDNNDALERCTIQRGMSYFFPPEVM
                     GAHIGYRRCHATFRQHSIAFRGLTALFGHMGLELDPVTADEMESDGYRRYALLYKEWR
                     QLIHTGVLWRVDMPDSSIQVQGVVSPDQSQALFMINQLAMPDYTLPGILRFPGLAAEV
                     RYRLRVIDHPEIQLVGEGGHTMRRLPVWMNQPLEVSGEWLAQGGIQLPVLDPESAILI
                     ALEKAV"
     gene            1086..1199
                     /locus_tag="ACHJ8C_004506"
                     /pseudo
     CDS             1086..1199
                     /locus_tag="ACHJ8C_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224356.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(1194..1493)
                     /locus_tag="ACHJ8C_004507"
                     /pseudo
     CDS             complement(1194..1493)
                     /locus_tag="ACHJ8C_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1492..1947
                     /locus_tag="ACHJ8C_004508"
                     /pseudo
     CDS             1492..1947
                     /locus_tag="ACHJ8C_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1980..2522
                     /locus_tag="ACHJ8C_004509"
                     /pseudo
     CDS             1980..2522
                     /locus_tag="ACHJ8C_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc48 family transposase"
     gene            2710..3273
                     /locus_tag="ACHJ8C_004510"
     CDS             2710..3273
                     /locus_tag="ACHJ8C_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HFO5554231.1"
                     /translation="MRKESGLSLIESAIVLSLAAAVTAGVLYYYNSANQSKLVNDTVA
                     QIQSIVSRLNAMYTSNPSAMFQEGADVTERNKVMEAVSELTGISLMEYSEGSKVLKNP
                     MGYGIQFWGTGFRKYQIETQVKDADTCTRLAVLDLGTVSYQGVRVYQGNTLKGSVSSV
                     QDASRYCSEADYNDNGNPVKVRFSIKF"
     gene            complement(3528..3921)
                     /locus_tag="ACHJ8C_004511"
                     /pseudo
     CDS             complement(3528..3921)
                     /locus_tag="ACHJ8C_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558160.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          974 a          899 c         1008 g         1040 t
ORIGIN      
        1 atggagtttg gtatatgggg tgaaccagaa atgattaacc cggattctga cctgtttcgt
       61 cttcatccgg actggatatt gtcaatgcct ggatattccc agccaaccgg aagatatcag
      121 tatgttctta acctgaatat tccggaggcc tttgcttata tttatgaacg tttcttatgg
      181 ttactgggag aacatccggt tgattatgtg aaatgggaca tgaatcgtga gcttgtacag
      241 gcggggcatg aaggccatgc tgcagcagat gcacagaccc gtcagttcta ccgattactt
      301 gatctccttc gtgaacgttt tccacatgtt gagtttgaat cctgtgcttc cggtgggggg
      361 cgtattgact tcgaagtcct gaaacgcaca caccggttct gggtatctga caataatgat
      421 gcactggaac gctgcaccat acagcgtggc atgagttact ttttccctcc ggaggtgatg
      481 ggggctcata ttggctatcg tcgctgccat gcaactttcc ggcagcacag catcgctttt
      541 cgtggtctga cggcattgtt cggccatatg gggttggagc tggatcctgt gaccgcagat
      601 gagatggaat ctgacggtta tcgtcggtat gccttgctct ataaagaatg gcgacaactg
      661 attcatacag gtgttctctg gcgtgtggat atgccggatt cttcgataca ggttcaggga
      721 gtagtcagcc ctgatcagtc tcaggcactt tttatgatca accagcttgc aatgccggat
      781 tacaccttac ctggcatact tcgttttccc ggactggcgg cagaagtgcg ttaccgactt
      841 cgggttattg atcacccgga aattcagctg gttggtgaag gtggccatac catgcgtaga
      901 ttaccagtct ggatgaacca gcccctcgag gtcagtggtg aatggctggc acagggaggg
      961 attcagctcc ccgtactgga tccggagagt gcaattttga tagcacttga aaaagctgtg
     1021 tgaagagagg tacggagcct cagctccgtc ctgtttctca atattatccc gtgagggtaa
     1081 gaataatgaa ttctgcaagt acgcataaaa atcccgattt ctggattttc ggtctgttct
     1141 tctttctcta ctttttcatc atggcaacct gttttccgtt tttggatttg cccctatatt
     1201 tccagacatc tgttatcact taacccatta caagcccgct gccgcagata ttcccgtggc
     1261 gagcgataac ccagcgcact atgcggatgc cattcgttat aatgctcgaa cgcctctgca
     1321 aggttctttg ctgccgttaa cccgtctggt ttgggcatga tactgatgta gtcacgcttt
     1381 atcgttttca cgaagctctc tgctattccg ttactctccg ggctccgcac cgccgtgttc
     1441 ttcggttcaa gtcccaacat ccgggcgaac tggcgtgttt cattagcccg gtatcttcgc
     1501 accgacgagg gctggctgta cctcgtggtg gtgattgacc tgtggtcgcg ggctgtcatc
     1561 ggctggtcga tgtcgtcgcg gatgacagcg catctggcct gcgatgcgct gcagatggca
     1621 ctctggcgac gtaagcgacc ggaaaatgtc attgttcaca ccgaccgtgg cggccagtac
     1681 tgttcaacgg attaccaggc cttgcttaag cggcacaatc tgcgcggaag catgagcgcc
     1741 aaaggatgtt gttacgacaa tgcctgcgcg gaaagcttct tccactcact gaaggtggaa
     1801 tgtatccacg gagaacgctt tatcagccgg gaaataatgc ggactacggt gtttaattat
     1861 attgagtgtg attacaatcg gtggcgccga cacagtgcct gtggcggtct cagtcccgaa
     1921 caatttgaaa accagaacct cgcttagggc cgtgtccaca ttacatgggt agtatcaggc
     1981 agcgggaaca taaaggccga atatcggtga aggaaagcga ccagcgttgg tgttccgatg
     2041 gctttgagtt cggttgcgat gatggcgaaa aactgcgtgt tacgttcgtc ctggactgtt
     2101 gcgatcgtga agccatagac tgggccgcaa gcacgggagg ttacgataaa gcgacagtgc
     2161 aggatgtgat gttaggagcg atagaaaaac gctttggcga taaagtaccg gaacagtcca
     2221 tccagtggct gacagataac ggttcagcgt acagagcgca tgaaacacgg cagttcgcca
     2281 gagagctgaa tctgaagtcc tgcacaacgg caatcagtag ccctcagagt aatggaatgg
     2341 cagaacggtt cgtgaaaacg atgaaggaag actacatcgc gttcatgccg aaaccgaatg
     2401 taagaacggc attgcataac cttgcagtgg cgatcgagca ttacaatgaa aaccatccgc
     2461 atagtgcgtt gggttatcgc tctccgcgag aatatcgacg tcagcgggta atgttaactt
     2521 aagatacaac ccctgtctgg aaataagggg gcaagaacac tctggattaa attcctgtcg
     2581 accaaggatc gcagtgctga tagttgtaaa aaaaagataa aaaaacatta atttatagcg
     2641 atcaggtcga cagcgaccgt taaacaattt cagaataaca aaggaatttt caatttttga
     2701 gaaaaagaaa tgagaaaaga atcaggatta tctttgattg aatcagcgat agtgctttcc
     2761 cttgctgctg cagtgactgc aggtgttctg tattattata atagtgcaaa ccaatcgaag
     2821 ttggttaacg atactgttgc gcaaattcaa agtatagtgt cccgactaaa tgcaatgtac
     2881 acatcgaatc ccagcgcaat gttccaggag ggagctgatg tgactgaacg caacaaggtt
     2941 atggaagctg tttctgagct tacaggtatt agcttaatgg agtatagcga ggggagcaag
     3001 gttcttaaaa atccaatggg gtatggtatc caattctggg gaactggttt cagaaaatac
     3061 caaattgaaa cccaggtaaa agatgctgat acttgcacaa gattagcagt tcttgactta
     3121 ggtactgtta gctatcaggg ggtgagagta tatcaaggca acacgttaaa aggttctgtt
     3181 tcatcagttc aagatgcttc ccggtattgc tctgaggctg attataatga taatggtaac
     3241 cccgttaagg ttcgattcag tataaagttc tgattgaagt tgtttctctg ctgacatgtg
     3301 cattagtcgc gacttaatct gacattgagc cttgaaaggt tgagagttat cggttttgat
     3361 atgagtgtct aatccttata caaaacacga gttaattctc atgcttcata ctaactgaac
     3421 cgcccggatt tcctggagag tgttttatct gtgaactcag gctgccggct cattgtttcc
     3481 ggtggaagca taataagctt tttctgcttc tgccggagga gtctggcccg ccatcccgcg
     3541 atgtgtccgg caaacacatc aatgatgaac gcccccctgc catgtactga cgtaagtaaa
     3601 atcagccacc cacaactggt caggacgttc tgccacgaac tgacggttta ctcggtcacc
     3661 tgcggcaacg gttttccggc tgacggttgt gcggaccttt ttaccccgga gaacaccggc
     3721 aagtcccata accgccatga ggcgcaccac tgtacatctg gccaccctga ttccttcccg
     3781 taacaactga cgccagactt tacgcacacc gtatacccgg tgattttcat cgtatacgcg
     3841 cagtatctct ttcttcagcc agtcatcgcg ctgcgcacgg gcactgcgtt tatcaggatg
     3901 atgtcgctgt tgctgacagt g
//
LOCUS       DAZFJL010000219         3812 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFJL010000219 DAZFJL010000000
VERSION     DAZFJL010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.221"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..2474
                     /locus_tag="ACHJ8C_004512"
     CDS             <1..2474
                     /locus_tag="ACHJ8C_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO5554232.1"
                     /translation="SKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFTWQFL
                     EQYPAAQDCAYKFLVCEDKPGMARPALDSWDTEYMQENRWDEESASFVPVEPEPNTEI
                     VNFNQLSDDKQAAVLVKFGTHENVTVDMVINASGLLGDDDMMTFDGHLAEAIIRTKEI
                     NAMYPERQIEYINDARRDLKPTAKWPDVQAYFLDRKKRQEKDRKEGGAYTSVVDLARA
                     RVNRQHTENSAEKINPVTTAIRREYKQTWKTLDEELAYSLWPGDIDAGNIDGSIHRWA
                     KNEVIENDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGRNQSATDTLSSPVPETDAVETAIPGNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYSGSQQNTGASDTTAQ
                     PEPVKVTADEVNKIMQAANISQPDANELLAASRGEFVAGISDPNDPKWVKGIETRDSV
                     NQNQQETEQNNQKAEQNSPNALQNEPETKQSEPVAQQEPEKVCTACGQSGGGNCPDCG
                     AVMGDATYQETFDEENQVEVQENDPEEMEGAEHPHKENPGGNQHHASDNKTGEATDPL
                     IKVNGHHKLTSTSRAGIHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LDTAGGVIDRDVIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNVILRRSYERQGIPCPWRYCNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSAIWQKLIPSQADF"
     gene            2533..2736
                     /locus_tag="ACHJ8C_004513"
     CDS             2533..2736
                     /locus_tag="ACHJ8C_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO5554233.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENGLPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            2736..3786
                     /locus_tag="ACHJ8C_004514"
                     /pseudo
     CDS             2736..3786
                     /locus_tag="ACHJ8C_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1200 a          874 c          914 g          824 t
ORIGIN      
        1 tgagtaaagt ctttatttgc gccgccattc cggacgaaca ggcaattaag gaagaaggtg
       61 cagttgctgt agccactgcc attgaagccg gtgacgaacg ccgcgcccgc gccaaattta
      121 cctggcaatt cctggagcaa tatccggctg ctcaggactg tgcttataaa tttcttgtct
      181 gcgaggataa acccggcatg gcccgccccg cccttgattc ctgggatacc gaatatatgc
      241 aggaaaaccg ctgggatgag gaatccgctt cctttgttcc ggtcgaacca gagccgaata
      301 cagaaattgt taactttaac cagttatccg acgacaagca ggccgctgtc ctcgttaagt
      361 tcggcacaca tgaaaacgtc accgtggata tggtcatcaa cgcaagcggg cttcttggtg
      421 atgacgacat gatgaccttt gacggacacc tcgcagaagc aattatccgg actaaagaaa
      481 ttaacgccat gtatccggaa cgtcagattg aatacatcaa cgatgccaga cgtgatttaa
      541 aaccaaccgc caaatggccc gacgttcagg catattttct cgaccgtaaa aaacgccagg
      601 aaaaggatcg caaagaaggt ggtgcatata cttctgttgt tgatcttgcc cgcgccagag
      661 tcaaccgaca gcacactgaa aactcagcag aaaaaatcaa ccctgtcact actgccattc
      721 gtcgcgaata caagcagaca tggaaaacgc tggatgaaga gctggcctac tctctgtggc
      781 ctggtgacat tgatgccgga aacattgacg gcagcatcca tcgctgggca aaaaatgaag
      841 ttatcgagaa cgatcgcgaa gactggaagc gtatttctgc atcaatgcgc aaacagcctg
      901 atgcccttcg ctacgaccgc cagactattt ttggccttgt ccgtgaacgt ccgatcgaca
      961 ttcacaaaga ccctgtggca ctgaacaaat acattactga atacctgact acaaagggcg
     1021 tgtttgaaga tgaaggaaga aatcagagcg caactgatac tctctcgtcg ccagtaccag
     1081 aaactgatgc agtggaaacg gcaattccgg gcaacgaaaa aaccgaatgc aaagtggaag
     1141 tcgaaccatc tgtagagcgt gaggggccgt tctacttcct cttcaccgac aaggatggcg
     1201 aaaaatacgg tcgcgcaaac aaactttctg gtctggataa ggcgctggct gccggggcta
     1261 ctgaaatcac gaaagaagaa tatttcgccc gcaaaaacgg tacatactca ggttcacaac
     1321 aaaatactgg tgcatctgac acgaccgcac aaccagagcc ggtaaaagtt acagctgacg
     1381 aagtaaacaa aattatgcag gcagccaata tcagccagcc tgacgctaat gaactgcttg
     1441 ctgcctctcg cggagaattt gttgcaggga ttagcgaccc gaatgatccg aaatgggtga
     1501 aggggattga aacccgcgat tctgtgaacc agaaccagca agaaacggaa cagaacaacc
     1561 agaaagcgga acaaaacagc ccaaatgcgt tacaaaacga gccagaaacg aaacaatccg
     1621 aaccggtagc gcaacaggaa ccggaaaaag tctgcaccgc ctgcggtcag agcggtggcg
     1681 gcaactgccc tgattgtggc gcggtgatgg gcgacgcaac ataccaggaa acattcgatg
     1741 aagagaatca ggttgaagtt caggaaaatg atccggagga aatggaaggc gctgaacatc
     1801 cacacaagga gaaccctggc ggcaatcagc atcacgccag cgataataaa actggcgagg
     1861 cgacagatcc cttaattaag gtgaatggtc atcataagct cacatccacc agcagagcgg
     1921 ggattcatct gatgatcgac cttgaaacca tgggaaaaaa tcccgatgcc ccgattatct
     1981 caataggcgc aatatttttc gatccacaaa ccggagatat gggaccggaa tttagcaaga
     2041 ccatcgatct ggatactgct ggcggagtca ttgatcgtga cgtcattaaa tggtggctaa
     2101 agcaatcacg tgaagcgcag tctgccatta tgaccgatga aatcccgtta gatgatgcac
     2161 tactgcaatt gcgggaattt atcgacgaaa actccggtga attttttgtt caggtctggg
     2221 gtaatggggc caacttcgac aatgtgattt tacgccgttc atacgaacga cagggtatcc
     2281 cctgcccgtg gcgctactgc aacgatcgcg atgtacgcac aatcgttgag ctggggaaag
     2341 ccatagactt cgatgccaga actgctatcc cattcgaagg tgagcgccat aatgcacttg
     2401 atgacgctcg ttaccaggca aaatacgttt cagctatctg gcaaaaactg atcccgagtc
     2461 aggctgattt ttaatgttca accctaattg ccgctaaccg tatatagtta gcggcggtta
     2521 tgagatatag ctatgagcag cttattttta accgaagatg aattgctaat attaacgggc
     2581 tgcaaatatg caagccacca gcgaaaatgg ttaatggaaa acgggcttcc gttctatacc
     2641 aatcgtagtg gcaaaccgat tgtcagccgg gatctattta cctgcaataa aactttacca
     2701 ccacgcgagg tagagccgaa ttttggtgcg atctgatggg aagacgaagg aaaaaccctg
     2761 aacacgaaaa attacctcca aatgtatacc caaataaata tagttatgta tggaaaccaa
     2821 catccagaga atctgtcaca ctaaccgcca tcaaggatgg tttagctgct ttatggaaaa
     2881 agtatgagga aactgtaaat aatcgcgatc gtgcaatgac attcggtcgc ttgtgggaaa
     2941 aattcctcgc cagcgcctat tacagtgacc ttagtccaag aacacaaaaa gattatctgc
     3001 aacatcaaaa aaagttgctt gccgtattcg gtaaggtacc agcggattcc ataaaaccag
     3061 aacacatccg tcgatacatg gacaaaagag gggagcagag taaaacgcaa gccaaccatg
     3121 aaaaaagcag tatgtcccgt gtttacagtt gggggtatga gcgagggtac gtgaaggcta
     3181 acccatgtgc aggtgtaagt aaattcaagg ccaaaaaccg cgaacgatat gtaaccgaca
     3241 aagaatacca ggcagtatta agcgttgcac ctcttcctgt ttttatcgca atggaaattg
     3301 cctatctgtg tgcagcgagg gtttccgatg tgttatcgct gaaatgggaa cagattggaa
     3361 acgacgggat attcatccag caagggaaaa ccggaaaaaa acagataaaa gcatggagtc
     3421 cacgattaca ggcagcgatc gaaaaagcaa aacagttacc aaaatctgcc tatgtgatca
     3481 gcaatcaata cggcaaccga tatatgtaca aaggctttaa cgaaatgtgg gtagatgcaa
     3541 gaaatcgtgc tggaaaaatt tcaggtattt taaccgactt cacctttcat gatctgaagg
     3601 cgaaaggaat ttcagactat gaaggaagca gccgggataa gcaacttttc tctggtcaca
     3661 aaaccgaagg gcaagtgcta atctatgaca ggaaggttaa agtttcacca acacttgatg
     3721 tcccgttacc tgaaaatatt ccaagaaaat attccaagta attccaagtg tgatttttgt
     3781 cactgactta atgatgtgta agtgattgaa tt
//
LOCUS       DAZFJL010000220         3804 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZFJL010000220 DAZFJL010000000
VERSION     DAZFJL010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.222"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            35..1420
                     /gene="dcuC"
                     /locus_tag="ACHJ8C_004515"
     CDS             35..1420
                     /gene="dcuC"
                     /locus_tag="ACHJ8C_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO5554234.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1461..2141)
                     /gene="dpiA"
                     /locus_tag="ACHJ8C_004516"
                     /gene_synonym="citB"
     CDS             complement(1461..2141)
                     /gene="dpiA"
                     /locus_tag="ACHJ8C_004516"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO5554235.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2110..3768)
                     /gene="dpiB"
                     /locus_tag="ACHJ8C_004517"
     CDS             complement(2110..3768)
                     /gene="dpiB"
                     /locus_tag="ACHJ8C_004517"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO5554236.1"
                     /translation="MLQLKENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLVMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKSVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT          872 a          997 c          918 g         1017 t
ORIGIN      
        1 ttatttactt tggggcctgg agacaggaaa aattatgctg acattcattg aactccttat
       61 tggggttgtg gttattgtgg gtgtagctcg ctacatcatt aaagggtatt ctgccactgg
      121 cgtgttattt gtcggtggcc tgttattgct gattatcagt gccattatgg ggcacaaagt
      181 gttaccgtcc agccaggctt caacaggcta cagcgccacg gatatcgttg aatacgttaa
      241 aatattgcta atgagccgcg gcggcgacct cggcatgatg attatgatgc tgtgtggctt
      301 tgccgcttac atgacccata tcggcgcgaa tgatatggtg gtcaagctgg cgtcaaaacc
      361 attgcagtat attaactccc cttaccttct gatgattgcc gcctattttg ttgcctgtct
      421 gatgtcactg gctgtctctt ccgcaaccgg tctgggtgtt ttgctgatgg caaccctgtt
      481 tccggtgatg gtaaacgttg gtatcagtcg tggcgcagct gctgccattt gtgcctcccc
      541 ggcggcgatt attctcgcac cgacttcagg ggatgtggtg ctggcggcgc aggcttccga
      601 aatgtcgctg attgacttcg ccttcaaaac aacgctgcct atctcaattg ctgcaattat
      661 cggcatggca atcgcccact tcttctggca acgttatctg gataaaaaag agcacatctc
      721 tcatgaaatg ttagatgtca gtgaaatcac caccactgcc cctgcgtttt atgccatttt
      781 gccgttcacg ccgatcatcg gagtgctgat ttttgacggc aaatggggtc cgcaattaca
      841 catcatcact attctggtga tttgtatgct aattgcctcc attctggagt tcatccgcag
      901 ctttaatacc cagaaagttt tctctggtct ggaagtggct tatcgcggta tggcagatgc
      961 atttgctaac gtggtgatgc tgctggttgc cgctggggta ttcgctcagg ggcttagcac
     1021 catcggcttt attcaaagtc tgatttctat cgctacctcg tttggttcgg cgagtatcat
     1081 cctgatgctg gtattggtga tcctgacaat gctggcggca gtcacgaccg gttcaggcaa
     1141 tgcgccgttt tatgcgtttg ttgagatgat cccgaaactg gcgcactcct ccggcattaa
     1201 cccggcgtat ttgactatcc cgatgctgca ggcgtcaaac ctgggtcgta ccctatcacc
     1261 cgtttctggc gtagtcgttg cggttgccgg gatggcgaag atctcgccgt ttgaagttgt
     1321 aaaacgcacc tcggtgccgg tgcttgttgg tttggtgatt gttatcgttg ctacagagct
     1381 gatggtgcca ggaacggcag cagcggtcac aggcaagtaa atagtcatgc cggaggcaag
     1441 tttcctccgg ctttatcatg tcacccactg tggtatatgc gttgcggtct gccaactttg
     1501 ccgtgaacaa tttcagcaat aatcagatgg cggctggcgc aatattcaag ataacgcctg
     1561 gcagtggtgc ggctgatggt cagtgcctgc gccaccgttt ctgccgtatg ttgcacacca
     1621 ggctctttga acagttttcg caccgcgttt agcgtcaagg ggtcaatgcc ggtcggcagt
     1681 tcgtctttgg gttcaccgcg agcataggca ttaaacatct catcaatttg cttctggctg
     1741 gcgctatcaa tactttccag catatgttta cgctggcgga aacgggttag cgtttgcccc
     1801 aaccgttcat aggcaatggg cttaatgaga taatcaaata caccacaacg tacggcttca
     1861 gacaccgttt ccatatcgct ggctgcagtg gtaaacacca cgtcgccggg ataatgcgcc
     1921 tgcaccagtt catgcagtaa attaatccct ctaccgtcag gaagatagtt atcgagcaag
     1981 attagccccg gcttaaaacg ctcgatcatc attcgggcct gcgccaggtt tcccgccagt
     2041 aatatctgac tgaatccggg aatgtgacga atatattccg catgcatctc tgccagcggc
     2101 gtttcgtcct caacgatcaa tagggttaat ggagctgtca ttaggtttca ctttcggaat
     2161 atagattgaa aataaggtac cgcagggatc attatcttcg agagtgataa caccaccgca
     2221 gcgcgttacg tagctggcaa tcaagtacaa cccaatgcca tgttcaccgg gctcgtcagc
     2281 acgcgtactg acaccctgct caaatatttt gtctcgtaga gactctggaa cgccgcagcc
     2341 ctgatcggcg acttcaatca ccacatcatc gccttcatcg ctgaggaata attcaacgct
     2401 cttgtttcct tcatcgctac gcaggcttgc ttcgaaggcg ttatcaagta aattgccaac
     2461 aatggctgca aactcggtgc tgtccagtcc tggcggcagt tgcgaaagct ggctaccggg
     2521 gacgatgacc atttttagcc ccagttcgcg ggcgcgctgc actttaccaa aaagcagccc
     2581 cgccacctgg cgatcggcaa acgcctcacg caggctgtca ataagctgtt gctgggcctg
     2641 agactccccc tgcaccatca ccagcacgcg atcatactct ttcatctgca acagaccatt
     2701 gagcgtcgac atccaattga gatgctcgtg tcgcaatgta cgaaggctct cgacgtattg
     2761 tttaatttgc gtcagttgcg cattgagggt ggaaatttcg tctttactac gaaagctaat
     2821 aatggccccc agtaaatcat cgccagaacg aatagcttcc cggttggcaa taacactcag
     2881 accgttaaag ttcgccacca catcctgacg tttttcatca atctgttcgg taaagaaatc
     2941 ggcgggcctg accacttcag caatgggttt acccaaccat tgccgtccgg gggagctcag
     3001 ccccagcatc tttcttgcgt tacgattgat ggcggtaatg tacccgtgcg gatccaccgc
     3061 aatcagccct tcataaaccg aactaaacag cgcctcttgc tgacggacca cgcgagcgat
     3121 ttgctttggc tccatgccca tcatctgccg acggatatgc gcggccagga accacgacag
     3181 caacatcaaa atccctaaca gcacgacaaa cacgcctgcc atcggtaata aaaactcagc
     3241 ccgccagcta tcgattttac tcaccagata gccaatcgac accacgccga tgacttttcc
     3301 atcgtcatca aagattggcg ttttggcacg catcgccatg ccaattgacc ctttcccggt
     3361 gatgaagtag ctctcccctt tctccagcgc gcccggtttg gtgaactgca taggataacc
     3421 aattttctcc ggattaggat ggtaaagacg aatcgagtgc cgatcgccaa tcaccacata
     3481 atcgaaatca gtatctcttt gtaatttgtc agcgatggtc gccagcctct tgtagtcacg
     3541 cgttttcact gcactgatga tactgtcatt agaagcaata atttttgcct gattcattgc
     3601 catgtcgcgc acatgaagcg ttaaatagtc ctcaaaactg gctgtaaaat attgcgccag
     3661 ggctgcaatg acaaatattg agaacaccag aatcagcaaa aagatacgca gcggaaatgc
     3721 cagtctttgg aaaaatgcaa actgtttatt ctctttaagc tgcaacattg ttttccttgc
     3781 gggttgtccc gagcctcttt gacg
//
LOCUS       DAZFJL010000221         3793 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZFJL010000221 DAZFJL010000000
VERSION     DAZFJL010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.223"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..342
                     /locus_tag="ACHJ8C_004518"
     CDS             <1..342
                     /locus_tag="ACHJ8C_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554237.1"
                     /translation="TKQAINTFSGIKQNFDQIASDHKKQLEILTKSAKPFSIKTAIAI
                     CALCTLCLSAAFSGATWYVAQHEKEASLRFYARAFLDMKKITEESMRKLPKSDQQNIK
                     LKLDEIDSRKP"
     gene            362..481
                     /locus_tag="ACHJ8C_004519"
                     /pseudo
     CDS             362..481
                     /locus_tag="ACHJ8C_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309191.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            816..1079
                     /locus_tag="ACHJ8C_004520"
     CDS             816..1079
                     /locus_tag="ACHJ8C_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554238.1"
                     /translation="MATLTKKERAWLNELQDVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            1090..1410
                     /locus_tag="ACHJ8C_004521"
     CDS             1090..1410
                     /locus_tag="ACHJ8C_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554239.1"
                     /translation="MTTFTDKELIKEIKERIGSLDVRDNIERRAYEIALASLEAEAPN
                     LPGGFTIEEAKELHENLVKSHVSKALSGEKMKKENRDADLRWIHGVIVQAAWFVKASL
                     GQNA"
     gene            complement(1541..1978)
                     /locus_tag="ACHJ8C_004522"
     CDS             complement(1541..1978)
                     /locus_tag="ACHJ8C_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO5554240.1"
                     /translation="MFFSVGVETPKDDHTAYGITVPVFDCFDFGCVSAADSQAEIPAM
                     AREAILAIVEEMVISGAHSVDDIHDEGCLTYSANPNYNHCDSWFVIDVDLSEIEGKQQ
                     RINISLPDVLIRRIDGYVRESGGVYKDRSHFLAQAARHELAYK"
     gene            complement(2003..2185)
                     /locus_tag="ACHJ8C_004523"
     CDS             complement(2003..2185)
                     /locus_tag="ACHJ8C_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO5554241.1"
                     /translation="MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKK
                     DLPLGTVRSIKKMAGI"
     gene            2482..2925
                     /locus_tag="ACHJ8C_004524"
     CDS             2482..2925
                     /locus_tag="ACHJ8C_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554242.1"
                     /translation="MTQLIPEKILEIIDDHDRAEKKQRNKIGFIYLCLCLAIIGVAAY
                     TFISTFSINVVTIIIMLFGVFMIVIFKSYLDSWSGIILSSDHILSILDKTKDYPEIKR
                     IVINRLLSGSILTGKDEEYIYSQLEKAQQSNEREKRLQAIKEYTS"
     gene            complement(3313..3591)
                     /locus_tag="ACHJ8C_004525"
     CDS             complement(3313..3591)
                     /locus_tag="ACHJ8C_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFO5554243.1"
                     /translation="MHFTTFLKKHFDIEKVVGTSDSGNDTESIYVYEKGNDCEPLFIL
                     HESWLNAEIKKCGVWTIGNIYSTLEHGKEYSEQELIKMIKEGKVISKY"
BASE COUNT         1228 a          807 c          745 g         1013 t
ORIGIN      
        1 accaaacaag caataaatac tttttctgga atcaaacaaa attttgatca aattgcttcc
       61 gaccataaaa aacaactaga gatactaact aaatcagcaa aaccattcag tatcaaaaca
      121 gccattgcaa tttgtgccct gtgcacatta tgcctgtctg cggcattttc tggcgcaacc
      181 tggtatgttg cacaacatga aaaagaggca agtctgcgct tctatgcaag agcatttctt
      241 gatatgaaaa aaataactga agagagcatg agaaaattac caaaatcaga ccagcaaaat
      301 ataaaactca aactagacga aattgattca agaaaaccat aaatatgaaa aggagtccct
      361 aatgaccact tttaccgacg aggacaaaga actaattaaa gaaatcagag agcgaattgg
      421 cagcctggac gtgcgagaca atattgagcg tcgggcttat gaaattgctc tggcatcgct
      481 ggaagcagag gcagttatgt tctgtatatc aggacaaaat gtagattcag aagaacatgt
      541 atcaaccagc aaagcggttg ttgatgcctg ggttgaagaa tggaatcagg ttgacggaag
      601 tcctggcgaa ccactgtaca aaactatgcc actctactat cacgctgcct tgccagcgcc
      661 ggtagtgcct gaaggactgg ttaaagcggt gcgtttttat gagcaggtaa aaaatgagaa
      721 tccggcagtc gaaaccgtag catggaaaga cgctgttgac tgggttctcg aagaggcttg
      781 tcaggctgta aacattggca tcaaaggaga gtgatatggc aactttaaca aaaaaagaac
      841 gggcatggtt gaacgaatta caggacgttc ttgatcgctg cccatcaccg aaaaaaattg
      901 gtttttacac cattggcgat aaaagcattt acctgtatga cctgcgccgc atggatgaaa
      961 tcatggaggc tcttgataat cgttcgtcga tggattggtg tgttgctgtc catgatatga
     1021 atgccggatt tgatgaaaag attttattcc cctcatcagt tgaaagcaca gcaggataag
     1081 gactaacaca tgacaacgtt caccgacaaa gaactgatta aagaaatcaa agagcgtata
     1141 ggcagcctgg acgtccgaga caatattgag cgtcgggctt atgaaattgc tctggcatca
     1201 ctggaagcag aagcgccaaa tttgcctggt ggtttcacaa ttgaagaagc aaaagaatta
     1261 cacgaaaatc tggtaaaaag tcacgtaagc aaggcattaa gtggcgaaaa aatgaaaaaa
     1321 gaaaatcgtg atgccgattt acgctggatt catggcgtaa tagttcaggc tgcgtggttt
     1381 gtgaaagcat cactagggca gaacgcatag tgcgtacttg cttttctcct ggcaacggtg
     1441 gatcttccgc ttcctcgtcg ctcgctcgct atgctcggtc gctggactgc ggcgagcggt
     1501 tagctatcaa aaaaaatccc cgcacaacgc ggggatagtt ttatttatat gcaagttcat
     1561 gacgtgccgc ctgagccaga aaatggctac gatctttgta tacgccacca ctttctctca
     1621 catatccatc aatacgacga atcaatacat caggcaatga aatattaata cgttgttgtt
     1681 tgccctcaat ttctgacaaa tcaacatcaa ttacaaacca gctatcacag tggttataat
     1741 ttgggttggc ggaataagtc aaacaccctt catcatgaat atcatcaaca gaatgagcac
     1801 cgcttattac catctcttcc acgattgcca atatcgcttc acgcgccatt gcgggtattt
     1861 ctgcctgaga atcagcagca gaaacacatc caaaatcaaa gcaatcaaaa accggaacgg
     1921 tgataccgta tgctgtatga tcatctttcg gtgtttcgac accaacagaa aagaacatgg
     1981 ctacctccaa agtcggcggg gattaaatcc ccgccatttt tttgatcgat ctgacagtac
     2041 ctagcggcaa gtccttttta ggatgaggaa caggaaaagt ttttcctgtt attggcgacc
     2101 accatatctg gtgactgctt gccttatgcc gtttcaactc gcaaccagca gctatcagtt
     2161 ctttcaacag gtcagcagat ttcatatgtc ctccctaacc tgaggataat tatacacaca
     2221 catacacaca aaggcaaatt atttcattcg tatgacttat gaaccacact gtaaagacag
     2281 ctcaatcttt acagtgtaag tcatttatcc agttttatcg ctgtgtatgt gatacataga
     2341 aaataatata gtattttact atatgatatc tgattgacac ttttaactgc acatctcatc
     2401 atttacatcg taaagacaaa catagattta caatgtaaag ctatgtaatt aaacactccg
     2461 tgtcccaaat aaggaaagaa aatgactcaa ctaatcccag aaaagatact ggaaatcata
     2521 gatgatcatg atcgtgcaga aaaaaagcaa cgcaacaaaa tcggtttcat ttatttatgt
     2581 ctctgtttag caataattgg tgtggccgcg tataccttta tatcaacgtt tagcattaat
     2641 gtggtaacaa tcatcataat gcttttcggt gtgtttatga ttgtgatttt taagagctat
     2701 ttagactcat ggagtgggat aattcttagc agtgaccaca tattgtccat actcgataaa
     2761 accaaagatt acccagaaat taaacgtata gttattaatc gtctgctctc cggctcaata
     2821 ctcacgggta aagatgaaga gtacatatac agccaactgg aaaaggccca acaaagcaac
     2881 gaacgtgaaa agagattgca agcaattaaa gagtatacat cgtaatacaa cgcattacgt
     2941 cgaaataccc tggatcatcg aagccacata cccctaagcg cagcgcaacc gccggaaaac
     3001 gcaccacact gtaaagacaa taaaatcttt acaacgtaaa gctaactatg taatacaacg
     3061 cgctacacaa taagaaaggg agagcatgac actctcccca tatatgactc aaactaaagc
     3121 attatcccgg ttatgcgccc accaaatgat ttcgctaata atccatgctt ctatcgcggg
     3181 tgtttttaca ggttccccat ttacaatgcg gtttgcaagc tctatcattt cacatactga
     3241 catttgagaa tctccaaaga tgcatctgtc atttggtaac aggctgcccc acagcctgtt
     3301 accaacccca aattaatatt tgctgattac tttaccctcc ttaatcattt ttattagttc
     3361 ctgttcagaa tattcttttc cgtgttccaa tgtggaatat atattgccaa tagtccatac
     3421 cccacacttt ttaatttctg catttagcca ggattcatgc agaataaaca acggttcgca
     3481 atcatttcct ttttcataaa cataaataga ttcggtatca tttccagaat cagaagtacc
     3541 aacgactttt tctatatcga aatgtttttt tagaaaagtt gtgaaatgca ttttcatgct
     3601 cctgccgctg agcggctttg gtggttgggg accatcccct cccgatgtgt ttataatagg
     3661 tcagaactga cctattatca agtatttttc cctccattct tcaaaaaaaa taccatcaaa
     3721 tacatatagt tatattcatt tgatgcccga cagggcatgg aaggggcgaa agaaaatacc
     3781 agggagcagc ggg
//
LOCUS       DAZFJL010000222         3787 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZFJL010000222 DAZFJL010000000
VERSION     DAZFJL010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.224"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(1..263)
                     /locus_tag="ACHJ8C_004526"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="ACHJ8C_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558160.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    complement(16..132)
                     /locus_tag="ACHJ8C_004526"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            272..445
                     /locus_tag="ACHJ8C_004527"
                     /pseudo
     CDS             272..445
                     /locus_tag="ACHJ8C_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(615..747)
                     /locus_tag="ACHJ8C_004528"
                     /pseudo
     CDS             complement(615..747)
                     /locus_tag="ACHJ8C_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
     gene            complement(748..1053)
                     /gene="ccdB"
                     /locus_tag="ACHJ8C_004529"
     CDS             complement(748..1053)
                     /gene="ccdB"
                     /locus_tag="ACHJ8C_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFO5554244.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1055..1273)
                     /gene="ccdA"
                     /locus_tag="ACHJ8C_004530"
     CDS             complement(1055..1273)
                     /gene="ccdA"
                     /locus_tag="ACHJ8C_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFO5554245.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVAWFIEMNGSFADENRDW"
     gene            1869..2113
                     /locus_tag="ACHJ8C_004531"
                     /pseudo
     CDS             1869..2113
                     /locus_tag="ACHJ8C_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2113..2703
                     /locus_tag="ACHJ8C_004532"
     CDS             2113..2703
                     /locus_tag="ACHJ8C_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554246.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRMEASDWLRTLRAPNLQLAVELTDAGRAVAQPLLQAEQERLRAEQR
                     AAEVVVLPLVPAADLPADGTSAKDLPVDLDGMTYQACRGDFVVRLDGSTCLQLWYKEG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQVNESTNP"
     gene            complement(2828..3691)
                     /locus_tag="ACHJ8C_004533"
     CDS             complement(2828..3691)
                     /locus_tag="ACHJ8C_004533"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO5554247.1"
                     /translation="MLNVQEKVSIVNELRQEWPLSRLLIVSGLPRSTFYYHVRRLAAP
                     DRYQSARALLLKIYHQHKGRYGYRRIRLACRNEGVLLNGKTVRKLMKELGISSLIRVK
                     KYRSYKGEQGRICDNLLKRNFDAKRPNEKWVTDVTEFKVNGKKLYLSPIMDLYNGEII
                     SYNLATRPQPSMVQTMLTDALKQLSKDEHPILHSDQGWQYQMSRWQRWLKDSGIVQSM
                     SRRGNCLDNAVIESFFGTLKSECYYLNEYKNVEDLKRDIIDYINYYNQLRIKEKLGGL
                     SPVQYRLSQAA"
BASE COUNT          945 a          953 c          960 g          929 t
ORIGIN      
        1 gtggtaatac gttgacgggg caatatgcag ttcgctgcat accggtccga ccccgtactg
       61 ctcacgcagc ttatccagca gtggcattat tttttccaga ggcggtcgaa ctccgccttc
      121 gcaaaataag cggaagcctg gcgaaggata tcgttactgc ggcgcagttc acgattttcg
      181 cgttccagct ctttcaggag ctgacgttca gcggtggtga gcccaccatc accgccccag
      241 gtatcccgct catgctggcg aaccggaagc agtggcgaaa gtgtgctatg cacaaatggt
      301 gaagcagttc ctgagtcgtg acccgttcga atgcgtgctg tgtggctgcc ggatggtata
      361 ccgccgggcc attgcggggc tgaatgtatc agggctgaag aaaaacgcgc gggatataag
      421 cctgctgagg tatatccctg gctgatgcag gagaggtgcg cctgcaatcc gggaaaagta
      481 taaaaaacgg cccattacgg tataaaaacc gaccatacat agagagaaaa caccggagtg
      541 gtgggaaaag cagaaaagct cacgtggcat aaggcgcgga caggggtatc gctcagtttt
      601 tcaatttcct atacgtgaac catcgacatc tggcagtgcc agagctgccg gataatcaat
      661 ggcaacaggc agcgatgcag gctgtccggc agaatacact gccggtacca tggctgcaga
      721 ctggctgtgt ataacggagc ctgacattta tattccccag aacatcaggt taatggcgtt
      781 tttgatgtca ttttcgcggt ggctgagatc ggccacttct tccccgataa cggagaccgg
      841 cacactggcc atatcggtgg tcatcatgcg ccagctttca tccccgacat gcaccaccgg
      901 gtaaagctca cgggagactt tgtctgacag caggcgggcg ctggccaggg ggatcaccat
      961 ccgtcgcccg ggcgtgtcaa taatgtcact ctggacatcc acaaacagac gataacggct
     1021 ctctctttta taggtgtaaa ccttaaactg catttcacca gtccctgttc tcatcggcaa
     1081 aagagccgtt catttcaata aaccaggcga cctcagccat cccttcctga ttttctgctt
     1141 tccagcgttc ggcacgcaga cgacgggctt cattctgcat ggtggtgctt accagaccgg
     1201 agatattgac atcatatgcc ttgagcaact gatagctgtc actgtcgacg gtgactgtaa
     1261 tgcgctgctt cataattcac ctcatcttac atactctctg cacacaatta agtatacacc
     1321 agtattacgc tatagcatgc ctccggcaac ggaatattgt tatcaggcta ttattaacac
     1381 tcacatcaat aaccaacata aaaagtgatc atggcaataa tagccggaaa tgtagtaacc
     1441 atgctaaatt acaggatgac tgtttttcca tgatccgatt tttatcgtat tcaggatttt
     1501 ccggaaatga atcacgatca gtgaataagc gaagcgatct tttctgtcgc tgatgactgg
     1561 ctcgggaaca cgggaagaaa cacaacatac agtgtcatgt cagcagagct gggcaccaca
     1621 taatgtgaaa gcgggccggc gggcggcagc gaaggatccg cggatccgga acttccggaa
     1681 acaggatctg cgcggcggga aacgggcagg caatatgata attaagcaat ttacataaaa
     1741 tcatgtcaga tgtgattttt atcattgatg ataaaaccat actagatatt atcataaatc
     1801 aataacagac aaatcatgca ggatatgatt ttttacctgt cagaatatta tcagatggag
     1861 cgtgcaggat ggatgaagaa cgggtgtttt cactcagcta tgaacaactg acccggtttg
     1921 cggaacgacg catccgggaa tgcaatctgg gcagccaggg cgcgatttat ctctgcgagt
     1981 cagccaaagc cggagccgtg ctgatcttct ggcatgaact ggcgcctcaa tgaatgccat
     2041 aaagagacag gagctcattg acgccgactt tcagcgcctc agaaagctca tctggccgga
     2101 ggatgacagg taatgaccca gagccgccga ccgtcgccac tgcagcggag agtgctgatt
     2161 gtgctggctg cccttgatga aaaacggccg ggcccagtgc tgacacggga catagaacgg
     2221 gtgctggagc gaagcgggga ggcccctgtc tacgggccaa acctgcgcgc ctcctgccgg
     2281 cgcatggaag catcagactg gctgcgcacc ctgcgggccc cgaatctgca gctggctgtc
     2341 gagctgacgg acgccggccg ggccgtggca cagcctctgc tgcaggctga acaggagcgc
     2401 ctccgggcag aacagcgcgc ggcggaagtc gtcgtactgc cgctggtccc ggcagcagat
     2461 ctgcccgcag acggcacgtc tgccaaagac ctgccggtgg atctggacgg catgacgtat
     2521 caggcctgcc gtggggattt tgtggtgcgt ctcgacggca gcacctgtct gcagttatgg
     2581 tataaagaag gcagagtggt acgccgggag ggcgatccac tggaagtggc gcagtggctg
     2641 caggcctgtc acgatgcagg aatggaggtt cgtgtgcagg ttaatgaaag caccaacccg
     2701 taaaaacatg cctgcacggt ttacacgtca tccgtaaccg gctcatttaa accgtctggt
     2761 ctgtttcctc cggctccaca aaaataatgt ccatcatttt tgaactgcgc cccagatcct
     2821 ggacaactta tgcagcctga ctcaatcgat attgtaccgg gctaagaccg ccaagttttt
     2881 cctttatcct caattgatta taatagttta tgtaatcaat gatgtctcgc tttaagtcct
     2941 ccacattttt atattcattg aggtaataac attctgattt caatgttcca aaaaagctct
     3001 ctataactgc attatcaaga caatttcccc ggcgagacat actctggact ataccgctat
     3061 cctttaacca tcgttgccac cgggacatct gatattgcca accttgatcg ctgtgcagta
     3121 tggggtgttc atctttcgac agctgtttca gcgcatccgt aagcatagtc tgcaccattg
     3181 aaggttgagg gcgagtagcg aggttatagg aaattatttc tccattataa agatccatga
     3241 tgggtgacaa atacagcttc ttgccattca ctttaaattc agtgacatct gtaacccact
     3301 tctcgttggg gcgttttgca tcaaaattgc gtttcaggag attatcgcaa attcgtcctt
     3361 gttctccttt atatgaacgg tattttttaa cgcggatcag gctgctaata cccagttcct
     3421 tcatcagttt tctgacggtt ttaccattaa gtaaaacccc ctcgttacga catgccagcc
     3481 tgatgcgtct gtaaccatag cggcctttgt gctggtgata aattttcagc agaagcgctc
     3541 tggcagactg ataccggtca ggagccgcaa gccgtctgac atgatagtaa aatgtactcc
     3601 ggggcaaccc ggaaacaatt aacaatcggg acaatggcca ttcctgcctt agttcattca
     3661 caatgctcac tttttcctga acgttgagca tgttttctgc tgaaccaagg ctttcagctt
     3721 ttttaagtag gtattctcga ccctgagttg ttcaagttct tctctctcat ctttggttaa
     3781 tgttggt
//
LOCUS       DAZFJL010000223         3775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFJL010000223 DAZFJL010000000
VERSION     DAZFJL010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.225"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..447)
                     /locus_tag="ACHJ8C_004534"
     CDS             complement(<1..447)
                     /locus_tag="ACHJ8C_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO5554248.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPTGFSSEQIHTTLVENLRYAANMLMKEDILLLIEPINHFD"
     gene            complement(460..2241)
                     /gene="gcl"
                     /locus_tag="ACHJ8C_004535"
     CDS             complement(460..2241)
                     /gene="gcl"
                     /locus_tag="ACHJ8C_004535"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO5554249.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKVLMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(2331..3146)
                     /gene="allR"
                     /locus_tag="ACHJ8C_004536"
     CDS             complement(2331..3146)
                     /gene="allR"
                     /locus_tag="ACHJ8C_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO5554250.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(3224..3706)
                     /gene="allA"
                     /locus_tag="ACHJ8C_004537"
     CDS             complement(3224..3706)
                     /gene="allA"
                     /locus_tag="ACHJ8C_004537"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO5554251.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQKRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
BASE COUNT          953 a         1063 c          860 g          899 t
ORIGIN      
        1 atcaaaatgg ttaataggtt caatcagtaa taaaatatct tctttcatca gcatattcgc
       61 ggcataacgc aggttttcta caagcgttgt gtgaatctgt tcactgctga aaccagtcgg
      121 cgttttaccg accagacagt taattttttt attacccaga gcacgagcat aacgaatcgc
      181 tgctgctacg ccatcccgaa actcttcttc acggccagga atacaggcaa taccccgctc
      241 gcccgccgcc cagtcacccg ctggtaaatt gtgcagcgta tgctcgagtt tattactcgc
      301 cagcacctgt tttaattctt caatgtcgta gtcataaggg aacataaatt caacgccgcg
      361 aaaaccacac tgcgcagctt tctcaaaacg ggcgagaaaa tcatattcac caaataacat
      421 cgataaatta gcagagaaac gtaacattat ttatctccct tattcatagt gcatgaaaca
      481 ggtttcagtc ggtgcatccg ctgcgttatc ggcgatatct tcaaattcca tgacgttatc
      541 cagttcactg cccatcgaaa tattagtcac acgctcgaga ataacttcca cgactaccgg
      601 tacccgatat tgcgccatta agacttttgc ctgttcaaag gctggtgcaa tatcttccgg
      661 tttgaaaacg cgaattgctt tacaacctaa accttccgct acttttacgt ggtcgacgcc
      721 ataaccgttc acttcgctgg agttaatatt ttcgaacgcg agctgtacgc agtaatccat
      781 atcaaaggca cgttgcgact ggcgaatcag acccagatag gcgttattga ccagtacatg
      841 gatgtacgga atgttgaact gtgcgccaac ggctaattct tcaatcagga actggaagtc
      901 gaagtcaccg gaaatcgcca ccacattgcg ctctggatca gcggcacaaa cccccagcgc
      961 cgccggaatc gtccagccca gcgggcctgc ctgcccacag ttgatccagt ggcggtcttt
     1021 aaagacatgc agcatttgtg ccgcagcgat ttgtgacagg ccaatggtgg tgacataaca
     1081 aacatcgcgg ccaaaggctt tgttcatctc ttcatacacg cgctgcggtt tcaccggcac
     1141 gttgtcgaag tgggttttgc gcagcaaagt gcgtttgcgc tgctggcagt cggcgaccca
     1201 ctctttacga cacggcagac gaccggcttt ttgcatctcc tgcgccactt caaccagcag
     1261 cgtcagcgcc gctttagcat cagagacaat gccaagatcc ggacacagta cgcggccaat
     1321 ttgcgtcggc tcaatatcga tatgaacgat tttgcgccct tcggtgtatt tctctaccga
     1381 accggtatga cggttggcaa aacggttacc gataccaaat accatgtcgg aagccagcag
     1441 cgttgcgtta ccgtaacgat gcgcggtttg cagacccacc atcccggcca tcagttcgtg
     1501 gtcatccggg atacaacccc agcccatcag cgttgggatc accggaacgc tggtcagttc
     1561 agcaaactgt tgtaacagtg cagctgcgtc agcattgatt accccgcctc cggcaacaat
     1621 caccggacgt tcggcctgga ttaacatttc tacagctttt tcgatctgca tacggctggc
     1681 agcaggtttg tagaccggca gcggttcgta catatcagga tcaaactcga tttccgcaac
     1741 ctgtacgtcg aacggtaaat ccaccagtac cggacccggt cgaccagaac gcatcaggtg
     1801 aaacgcctgt tgcagcacac gaggcaccag cgccgcttca cgaactgtaa ccgccatttt
     1861 gctgaccggt ttagcaattg cttcaatatc aacggcctgg aaatcttctt tatgcagacg
     1921 ggcgcgcgga gcctggccgg taatacacag aataggaatg gaatcagcag aagcggaata
     1981 gagcgcggtg atcatgtccg tgcccgcagg accggaagtc cccagacata cgccgatatt
     2041 ccctgccgtt gcgcgggtat aaccttccgc catgtgcgaa gcaccttcca catgacgcgc
     2101 cagaatgtga cgaataccgc cgtgcttacg catcgctgag tagaacggat tgattgcagc
     2161 tcccggaaca ccgaaggcgg tggtgatacc ttctttctcc agcacataca ttgccgcgtc
     2221 aacggctctc atttttgcca ttttattcct acctctatta attggaaaaa ttttccaact
     2281 ctcaaatttt gtcaaaaaaa cccgctccgc agagcgggat acgacagaca ctatggatgt
     2341 gctttcagtc ccaacgccgt gctgatatcg cgggcggtgt ctctgaccag ctcaccctgg
     2401 ctgacaaaac gatcttctgt cagtcttgat gaaggcccgg agatggagat agcggcaaca
     2461 acactaccta catcatcgta aattgctgaa gctatacaat tcaggccgac aacatgctct
     2521 tctttatcta cggtatagcc cagttcacgc gcttgttcca ggtccttcag caaggtgggc
     2581 atatccacaa gcgtcgttgg cgtaaactgc tgcaaaccgg tttgcagaat gatgctcatc
     2641 aactcttctt ccgccagcgg ataaagcagc gccttgcccg ctccggaagc atgcagcggc
     2701 agacgactgc ccagcggcgc acacatcctg accatcgatt tacactctaa ctgaccaatt
     2761 aataccgctt cattgccgtt acggatcgcg acattgaccg tttcgccgga aagtaacatc
     2821 aggcggcgca taaacggccc ggcgacggag agtacatcgc ggttatggat gtacgctgca
     2881 ccgacgttaa agacacctaa tcctatatgc caccagccta actgactgtc ctgatagaca
     2941 aaatccgctg cctgtaaaac cttcagcaag cgaaaggtcg tggagagcgg caaatccaga
     3001 ttgagagaga tatcgctaac cgacgaactt cccccacttt tttccagata ttgcagaatc
     3061 gcaattcccc gctctaacgc ctgtgcaccc ttctgtgcca caggctccgc ctgtcctggc
     3121 ctgccgcgcc gtctaacttc cgtcatacct acgttaactc cgacctacaa atgagcataa
     3181 acaatgaaca attcgtttat cttatgcaag tcggttgcag gcgtcacgca aaacagagtt
     3241 cctgttcagg aatactttca acatcacagt tgtcactgcc gccgcgatcg atggtcagaa
     3301 aatcggtgac gcgctgccag gcgaaaagtg ggtgatgcca gacgttacga tggtaattca
     3361 ctccctgttc gccattggta ataaacgccc gcagggttga caggtctggt ttgtcgtcac
     3421 ctaacgccac caccaccaca aacacttcac ctttcatcgg gataaacgcc tgtgtaccca
     3481 gcggatgacg ttcgagttcg tgaatagtca gcggcagatt cgccggttgc gcgcggttaa
     3541 tgctgataag cgtacggtct tgctcaagaa tctcaaccag cgccaaatcg tggtaacgct
     3601 ccaccagtcc attgttaata tggaaaaaat cccgtttctg cgtttcgatt acgtcgccat
     3661 aagcactaaa ggcttcctga cttaacggta atacctgaag tttcatcttt ttgtccttgt
     3721 aacataattc cgttcaacca ccgccgaccg gtcagcttgg tttcaataac aggca
//
LOCUS       DAZFJL010000224         3739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFJL010000224 DAZFJL010000000
VERSION     DAZFJL010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.226"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..358)
                     /locus_tag="ACHJ8C_004538"
     CDS             complement(<1..358)
                     /locus_tag="ACHJ8C_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO5554252.1"
                     /translation="MAKILPSIFGADILRLKDEIHFLEEEQTEILHVDLMDGTYVSNI
                     AFGPNQIAAMKKASSMIFDVHMMLANPERHIDDVINTGAEMISVHYESTPHVHYIIQK
                     IKKAGRKAGVVLNPGTP"
     gene            complement(381..1634)
                     /locus_tag="ACHJ8C_004539"
     CDS             complement(381..1634)
                     /locus_tag="ACHJ8C_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFO5554253.1"
                     /translation="MEMLKNLMETITGMGATAILPLVIFILGLVFRMKPGAAIKSGIT
                     VGIGFIGLGLVVGLLNSSLQPAIEYYSKVGSGFTVADIGWPAVGAAAWVAPFAALVIP
                     VGIVLNLILVRLKLTKTLNVDIWNYMHFLVPGALAYFVFDSFIIGFSVAVALSIAALF
                     IGDLIAPRWQKYYGLEGTTCTTMIHIGWTLPFAWVVNKIIDYIPGLNKLDVDLNSVQK
                     RLGVFGEPAIIGIIVGALLGVLTKQAITTIVPMAMGVAGVMVLLPKVVGVLMEGLNPI
                     GKSAKEIMQKQMGKDAELNIGMDCALALGDPATVTVTVITIPLTMLCALVLPDIKIFP
                     IGVLMSIIYMTTMTVMVSNGNVVRSIISTLLFCVVVMYLGGYVAPGATQFLAGAGVGL
                     QGQGTDFVLTGPWEILTYWLSTVLH"
     gene            complement(1655..1948)
                     /locus_tag="ACHJ8C_004540"
     CDS             complement(1655..1948)
                     /locus_tag="ACHJ8C_004540"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO5554254.1"
                     /translation="MINILVACGSGVATSTLAADEVKSVCAEYGITAFKINKCSMAEL
                     SSEMAHADIVLTTNNYKGDIGKPHMSVAGFVTGINEGALRKKLGELLTELVNN"
     gene            complement(1967..2410)
                     /locus_tag="ACHJ8C_004541"
     CDS             complement(1967..2410)
                     /locus_tag="ACHJ8C_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO5554255.1"
                     /translation="MISISVADGAGLQNYDAVLEHIFNTHRTTNTVKESWLSAMRERE
                     AEYPTGIELEGYSIAIPHCDSEHASQPAIYIIRLPDAIPVNRADEDGILWVRLVVNLI
                     VTDPADQLRLLKSLFNHLQNEEFYQNLLTLPVEDAKALFVSKIIQ"
     gene            complement(2749..3504)
                     /locus_tag="ACHJ8C_004542"
     CDS             complement(2749..3504)
                     /locus_tag="ACHJ8C_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5554256.1"
                     /translation="MYLVDERRKLILEDLMSAGTVYVSDLARKYEVTYETIRKDLTHL
                     EQKGLLIKSHGGATLKQRAIEHPFQVREKENNSYKKAIARKALDFIPPNSSMIIGTGS
                     TTFELANLLCMKSGFKIFTDSLPVASVLIESDNQVFLFGGELRRKSSSVFGGWAVSMI
                     NDIHVDLCFIGTDGFSNMTGPSTPSSSDAFLDRTIISHSDKKYILGDYTKFERSSLFK
                     ICDWSEITALITNDHADKEGIAEVAKYTSVITC"
BASE COUNT         1137 a          864 c          685 g         1053 t
ORIGIN      
        1 caggtgtccc aggattaagc acaacacctg ctttgcgccc cgcttttttg attttttgaa
       61 taatataatg tacatgtggc gtagattcat aatgtacaga aatcatctcc gccccggtat
      121 tgattacatc atcaatatgt cgctcaggat ttgccagcat catatgcaca tcaaagatca
      181 tcgaactggc ttttttcatt gctgcaattt gatttgggcc aaaggcaata ttactgacgt
      241 aagtgccatc cattaaatca acgtgcaata tttcagtttg ttcctcttcc agaaaatgaa
      301 tttcatcttt caggcgtaaa atatccgcgc caaatataga aggtaatatt tttgccattt
      361 aaaaactcca gattattaaa tcaatgtaat acggtgctca accaataggt taaaatttcc
      421 cacgggccgg ttaatacaaa atctgtacct tgtccttgca agcctacacc ggctccagct
      481 aaaaattgcg ttgcccctgg tgcgacataa ccgcctaaat acatcactac aacgcagaat
      541 aacaaggtcg agataatcga acgaaccacg ttaccattgc tgaccattac ggtcatggtg
      601 gtcatataaa taattgacat caatacgcca attgggaaga tcttaatatc aggtaatacc
      661 agagcacata gcatggttaa aggaatggta atgactgtca cggtgaccgt cgccggatcc
      721 cccaatgcca gtgcacaatc cataccgatg tttaattcag catctttacc catctgtttt
      781 tgcatgattt ctttggcact tttcccaatc gggttaagac cttccatcag cacaccgacc
      841 actttaggca ataataccat tacgccagca acccccatcg ccatgggaac aattgtggta
      901 atggcctgtt tcgttaaaac tccgagtaat gcgccaacga taataccgat aattgcaggt
      961 tcaccaaaga cacccaggcg tttttgcacg ctgtttaagt caacatctaa cttatttaac
     1021 ccggggatgt aatcaatgat tttatttact acccaggcga acggtagagt ccagccgata
     1081 tgaatcatcg tggtacaggt cgttccctcg agtccataat atttttgcca tctgggcgca
     1141 atcaaatcgc caataaacag agccgcaata ctcaaagcca ccgcgacaga gaaaccgatg
     1201 ataaaactgt cgaacacaaa atatgccaga gcacccggaa ctaaaaaatg catatagttc
     1261 cagatatcaa cattcaacgt tttggttaat ttcaggcgca ccagaatcag gttaagtacg
     1321 atgccaactg gtatcactaa tgcggcaaaa ggtgctaccc atgcagcagc accgacggct
     1381 ggccaaccaa tatctgcaac cgtaaaacca ctgcctactt ttgagtaata ctcgatagct
     1441 ggctgtaatg aactgtttaa taaaccaaca accaaaccta aaccaataaa accaataccg
     1501 acggtgatgc ccgacttgat cgcggcacct ggtttcattc tgaacaccag acctaaaata
     1561 aatatcacca gcggcagtat tgcagtcgcc cccattccgg tgattgtttc cattaaattt
     1621 ttcaacattt ccatttttat tattcctgtc aggtttagtt atttactaat tcagttaata
     1681 actctcctaa tttcttgcgt aatgctcctt cattaatacc cgttacaaaa cctgcaacgc
     1741 tcatatgtgg tttcccaata tcacctttat aattattagt tgtgagtaca atatctgcat
     1801 gagccatctc agaagaaagt tcagccatac tgcatttgtt gattttaaat gccgtaatac
     1861 catattcagc acatactgac ttcacttcat cagcagcaag cgtagatgta gcgacaccgc
     1921 taccacaagc aacaagaata ttaatcataa taaactccag tttttactat tggattattt
     1981 tggacacaaa aagtgctttt gcatcctcga cgggaagtgt cagtaagttt tggtaaaatt
     2041 cttcattttg gagatggtta aataatgact ttaacaatcg aagctggtca gcaggatccg
     2101 taacaataag attaacgacg agtctgaccc acaatattcc atcttcatca gccctgttca
     2161 caggtattgc gtctggcaga cggatgatat aaatagccgg ttgactcgca tgttcactat
     2221 cacagtgtgg tatagcgatc gaatagcctt ccagttctat tcctgtcgga tattccgctt
     2281 cgcgttcacg catagcactg agccagctct ctttcaccgt attcgttgta cggtgcgtgt
     2341 taaaaatatg ctcaagcacg gcatcgtagt tctgtaaccc cgcaccatcg gcaacgctga
     2401 tcgatatcat attcttgcac tctcattcgc tattgctcat atgagcaatt cttgattttc
     2461 atatgagcag ttataacaca acgatcctgc ggataaacag agagatctga gaagttatac
     2521 ttagatcaca aaaaacacat aatggcataa ttacttatgc tcatatgagc aattcatcat
     2581 gtgcgaaatg atttcttcgc gaaaaaggaa gtaaaactga caataagtag aggacgaatt
     2641 gaatagagat cgataagtaa gcatcaccag ggatgctcac ttgataattg ggaaatgatt
     2701 acatttggct aagctgattc gaagggacaa ggcgtgtcgt agtaagttct agcaggttat
     2761 tactgacgtg tattttgcga cttcggcaat cccctctttg tcggcatgat cgttggtaat
     2821 aagggcggtg atctccgacc agtcgcagat cttaaacaaa ctgctgcgct caaacttggt
     2881 gtaatctccg aggatatatt ttttgtctga gtgcgaaatt atcgtgcgat ccaaaaacgc
     2941 atcagaagaa gaaggcgttg atggaccagt catgttacta aagccgtcgg ttccaatgaa
     3001 acagagatca acatgtatat cgttgatcat agataccgcc caccccccaa acactgagga
     3061 actctttctt cgcagttcgc cgccaaagag aaaaacctgg ttgtcagatt caatcaacac
     3121 actggcaacg gggagcgaat cagtgaagat tttaaaccca gacttcatac ataacaggtt
     3181 agccagctca aaagtggtac tgcccgtacc gataatcatc gaactattag gcggaataaa
     3241 atcaagcgct ttacgagcaa tcgccttctt ataagagtta ttctcttttt ccctgacctg
     3301 aaagggatgc tctatggccc tttgctttaa agtcgcccca ccatggcttt tgatcagcag
     3361 ccctttttgt tcaagatggg tcaaatcttt cctgatggtt tcatacgtta cttcatactt
     3421 tcttgcgaga tcactgacat agaccgtacc tgccgacatt aagtcttcaa gtattaactt
     3481 tcgcctttca tcaaccagat acatccttcc tcctgaaatc tattgcccat ctgagaaaac
     3541 gccaagcata gcacagatga tgcaaacaaa aacaaacact aaccgtggaa agtacaaata
     3601 agcccaataa agacaatagt tcaaaagcca tatatccaat tgataataaa tgaattttta
     3661 ttattcatta caacgccact gtacaatccc aatttgtctc aattggctta atttattttt
     3721 tatttgtttt tattggtct
//
LOCUS       DAZFJL010000225         3714 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFJL010000225 DAZFJL010000000
VERSION     DAZFJL010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.227"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            163..222
                     /gene="ypfM"
                     /locus_tag="ACHJ8C_004543"
     CDS             163..222
                     /gene="ypfM"
                     /locus_tag="ACHJ8C_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO5554257.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(321..3434)
                     /gene="acrD"
                     /locus_tag="ACHJ8C_004544"
     CDS             complement(321..3434)
                     /gene="acrD"
                     /locus_tag="ACHJ8C_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO5554258.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
BASE COUNT          919 a         1001 c          933 g          861 t
ORIGIN      
        1 tctcgaaatg ttgcgcggta tcacatacaa atcatcaccg caggaccata gtatttacat
       61 aaggtttgtc ccccggaggt agtcatcgca gcaatcgagt aaattagctt caccacggta
      121 tattgtttcc gtagaatacc cataattata agagaggttg ttatgattga acgtgaactg
      181 gggaactgga aagactttat cgaagttatg cttcgtaagt aattcctgag acaaagtaca
      241 aacagcaaga acccgctgag caggttctta atcggaaagt tgaaaagggc gacacattgg
      301 catgtcgcct tttttattgc ttattccggg cgcggcttca gcgggaagcg gcggcgcacc
      361 agcacaaaga acagcggcac gaagtaaata gccagaatag tggccgaaat catcccgccc
      421 attacgccag tacccaccgc atgctgacca ccggaaccgg cacctgtgct ggttgccatt
      481 ggcaatacgc cgaagataaa tgccagcgag gtcatcagaa tcgggcgtaa acgctgacgg
      541 caggcgtgga gcgtcgcttc aaacaggtcg tggccttttt ggttcatctc gttagcaaac
      601 tcgacgatca ggatagcgtt tttcgccgat aaaccaatga ccgttaacag acccacctgg
      661 aagtaaacat cgttttccag cccgcgcatc caggttgcca gcagcgcgcc gataaccccc
      721 agcggcacga ccagcattac cgagaacggc accgaccagc tttcatacaa tgccgccaga
      781 cacaggaata ccaccagcaa ggaaatggcg tacagcgccg gagcctgcgc gccggaaagc
      841 cgctcctgat acgacatcgc cgtccactcc agaccaaagc cgtttggcag ctgcttcact
      901 aacgattcca taatatccat cgccgtaccg gtactgaccc ccggtgcggc ttccccaaca
      961 atctcaaccg cagaataacc attatagcgt tccagacgcg gtgagcctgt ctcccagcgt
     1021 gaggtcgcga aagcagagaa gggcaccatg ccgccatctt tatttcggac ataccagaga
     1081 ttgatgtcat ctggcagcat gcgatacggc gcagctgcct gcacatagac tttcttcacg
     1141 cgaccgcgat ccataaagtc attcacatag ctcgaacccc aggcggtttg cagtgtgtcg
     1201 ttaatatcgt cgatagcaac gcccagcgcc tgagctttac gctggtcgat atcaatctgc
     1261 aactgcggac tgtcgtcgag gccgttatgg cgcacacggg ttagctccgg gttttccgct
     1321 gccagcgcca gcaactggtt tcgtgctgcc atcagcgcat cgtgacccgc tccagcgtgg
     1381 tcctgcaact ccatatcaaa acccgcagaa ctaccaagac cgctaattgc tggcgggctg
     1441 ctggcgataa cgcgagcttc tttaatcttg ttaaacgcct tcgttgcacg ctcgataatg
     1501 gcaaacgagg tgccggtctt actgtcgcgt tcgctccagt ctttcaggcg gataaacatt
     1561 cgcgccacgt tttgcccgtt acccccaggg ccagaaccca cggtggcaaa caccgacatg
     1621 atgttgtctt tttcatgggt gaagtaatat ttctcgattt gctcaacgac tttcagggtc
     1681 tgttgctggg ttgaaccgct gggcaactgt accgaggtag taaacatgcc acggtcttcc
     1741 agcggtaaga acgacgtcgg caaacgcagg aacaggaaca ccatgccgcc aagcagcagg
     1801 acataaatca caatccagcg caggctacgg tggagaattt tcgccacccc tttttcgtag
     1861 cgttcggcgt tgcggttaaa catctggtta aaccaggcaa aaaagccttt ttgcccgtga
     1921 tgctcacctt ttttcagtgg cttaagtagc gtggcacaca gagccggagt gaggatcatc
     1981 gctaccagta ctgacagcac catcgccgca acaatggtaa tagagaactg gcgatagatg
     2041 gcaccggtgg tgccgccgaa gaaggccatt ggcacaaata ccgccgaaag caccatcgca
     2101 atcccgacca gtgccccctg gatctgcccc atcgatttac gtgtggcttc gcgaggagtg
     2161 agtccttcct cactcataat acgttcgacg ttttccacca ccacgatggc atcatccacc
     2221 agcagaccga tcgccagcac catcgcgaac atggttaagg tgttgacact gtaaccgaag
     2281 gcgtaaagca cggagaaggt tcccatcaac accaccggca cggcgatagt gggtatcagc
     2341 gtggcgcgga agttttgcag gaacagatac ataacgagga aaaccagggc gatagcttcc
     2401 agaagcgttt tcaccacgtc ttcaatggag gctttaacaa atgaggtggt ttcatacgcc
     2461 accttgtatt ccagtccgtg cggaaaatac tgcgccagct cgtcgagacg attgagcacc
     2521 agctctgctg tcgccatttc gttcgcgccg gaggccagtt ttacccccag cccggaggct
     2581 ggcttaccat tgaagcggct aagataatcg tatttttccg cccccatttc gacggtggcg
     2641 acatcaccca gccttacctc tgagccgtcc tgattcaccc gcaaggtgat atcgcggaac
     2701 tgttctggtg tttgcagcag tgactgggcg ttaatggtgg cgttgagcgc ctgcttatcg
     2761 acggaaggcg taccaccaag ttgcccaacc gcaatctgcg cgttctgtga ttcaatggca
     2821 tcggtgacat ctttagccgt catctggaaa ctgttgagtt tcgccgggtc cagccagata
     2881 cgcatggaat attgcgaacc ataggcatcg atatccccga cgccattcac gcggcttaac
     2941 gggtcctgaa tattactggc aacataatcg gcaatatcct gtttatccat cgaaccatcg
     3001 gtagagacga aggcaatggt cagaatgttg gtatcgccgg ttttacgcac cgtcacgccc
     3061 tgattttgca ccgcctgcgg taactttcgc atggctgatt gcagctggtt ttgtacttgc
     3121 tgcacggctt cgtccggatc ggtgcctgct ttaaaactta acgtgacaga tgcctgaccg
     3181 gtgccgctgc tctgagatga catatacatc agattatcga ggccggtcat attttgctcg
     3241 ataacctggg tcacggtgtt ttccagcgtc tgggccgatg cgccgggata gttagcggtc
     3301 actcgcacat tcggtggcgc gagatcgggg tattgttcaa cgggcaatga aaaaatcgcc
     3361 agggtacctg tcagacacaa caggattgcc agcacccagg caaaaatggg gcgatcaata
     3421 aagaaattcg ccattaaaag agaacctcgt gtttctgcgt atcgttgtag gcttgacttg
     3481 cttcactgta gcggcaaggt acgagccaat cgtggagaaa aaaaggagtt aatgtaaatc
     3541 tacgaaatgt gttgctatcg aaacatgcag catgaaaata gcaaaattgt tccctcatat
     3601 gccatgcatt ttatgttatt ggttatattc tattgatttg tttagtagtt ttcactcttt
     3661 agacgataaa tgtagcgaat caggccattt tgttttgttc attagcctca taat
//
LOCUS       DAZFJL010000226         3604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZFJL010000226 DAZFJL010000000
VERSION     DAZFJL010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.228"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..104)
                     /locus_tag="ACHJ8C_004545"
     CDS             complement(<1..104)
                     /locus_tag="ACHJ8C_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO5554259.1"
                     /translation="MSRVTAIIYVLVICLIVCLSWAVNHYRDNAISYK"
     gene            complement(101..598)
                     /gene="rrrD"
                     /locus_tag="ACHJ8C_004546"
     CDS             complement(101..598)
                     /gene="rrrD"
                     /locus_tag="ACHJ8C_004546"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO5554260.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDIIPGKTYTEAECKALLNKDLAMVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(598..813)
                     /locus_tag="ACHJ8C_004547"
     CDS             complement(598..813)
                     /locus_tag="ACHJ8C_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5554261.1"
                     /translation="MKSMDKISTSIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDRRKAARGE"
     gene            complement(1106..1180)
                     /locus_tag="ACHJ8C_004548"
     tRNA            complement(1106..1180)
                     /locus_tag="ACHJ8C_004548"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1145..1147),aa:Gly,seq:tcc)
     gene            complement(1184..1259)
                     /locus_tag="ACHJ8C_004549"
     tRNA            complement(1184..1259)
                     /locus_tag="ACHJ8C_004549"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1224..1226),aa:Thr,seq:tgt)
     gene            complement(1773..2036)
                     /gene="stxB2f"
                     /locus_tag="ACHJ8C_004550"
     CDS             complement(1773..2036)
                     /gene="stxB2f"
                     /locus_tag="ACHJ8C_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2f subunit B"
                     /protein_id="HFO5554262.1"
                     /translation="MKKMIIAVLFGLFSANSMAADCAVGKIEFSKYNEDDTFTVKVSG
                     REYWTNRWNLQPLLQSAQLTGMTVTIISNTCSSGSGFAQVKFN"
     gene            complement(2049..3008)
                     /gene="stxA2f"
                     /locus_tag="ACHJ8C_004551"
     CDS             complement(2049..3008)
                     /gene="stxA2f"
                     /locus_tag="ACHJ8C_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001476133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2f subunit A"
                     /protein_id="HFO5554263.1"
                     /translation="MRYILLKLVLFFCVCLSSASYADEFTVDFSSQKSYVDSLNSIRS
                     AISTPLGNISQGGVSVSVINHVPGGNYISLNVRGLDPYSERFNHLRLIMERNNLYVAG
                     FINTETNTFYRFSDFSHISVPDVITVSMTTDSSYSSLQRIADLERTGMQIGRHSLVGS
                     YLDLMEFRGRSMTRASSRAMLRFVTVIAEALRFRQIQRGFRPALSEASPLYTMTAQDV
                     DLTLNWGRISNVLPEYRGEEGVRIGRISFNSLSAILGSVAVILNCHSTGSYSVRSVSQ
                     KQKTECQIVGDRAAIKVNNVLWEANTIAALLNRKPQDLTEPNQ"
     gene            complement(3299..>3604)
                     /locus_tag="ACHJ8C_004552"
     CDS             complement(3299..>3604)
                     /locus_tag="ACHJ8C_004552"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554264.1"
                     /translation="KLLNSFVYSGDKVWSGYNFLPVFKFLDAVFSSEKNHESLHLKVD
                     RLFYRHKNENTTSYFITEYLVKMGNFATQLKHAAGRFFYVRKPVVVPGGGIVNGRSS"
BASE COUNT         1085 a          857 c          721 g          941 t
ORIGIN      
        1 tctttgtagg agatggcgtt atcacggtaa tgattaacag cccatgacag gcagacgatg
       61 aggcagatga ccagaacgta gataatcgcg gttactctgc tcattgttgc ccccacaaac
      121 agacttcacg ctcaatctca cggcgagtca tcagcccttt ccattgctta ccgccagcgt
      181 atgtccagcg ccgtagctga tcacatgcgc ctttgatatc gccctggttt attttgcgaa
      241 gaagcgtcga tgttctgaaa ttgccagcgc ccacgttgta aacgaacgag taaagagcgc
      301 cgcgcgttgt ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgaccatgg
      361 caaggtcttt attcaggagg gctttgcatt ctgcttcggt atacgtttta ccgggaatga
      421 tgtcttttcc ggtgtgtccg tgacatacag tccatacgcc aacgatatct tcgtatggta
      481 tgtagctgac accttccagg ccatcgtcac cactcggacc agtgatgagc acagacgcta
      541 tggcaatagc cccgccacca atagcagctg caacagcctt gcgtaatgat ggaggcatta
      601 ttcacctctc gcagcctttc ttctgtcttc tctgattttg aagtacagat ttgtcagata
      661 agtcaggaag cccagaacca gactccccag tacaccaatc gcagcccact gtgacggact
      721 gacctgatca agccactgta aaaaccagta gccagcactg cctgcggagg tgccgtaggc
      781 aatgctcgtt gaaattttgt ccatggattt catagcctca cctccgcaaa taacggatgg
      841 cgtagtttta cactgagaaa tgaaagggat ttgaaaagaa aaaaccgcaa aagcgggcga
      901 aacgatatat acagtaagga aagcactcta tccaacaaac cacccacagt taatcggaat
      961 aaaagcagag tgcttatgaa tgatcgcctg cccgaaagtt agtatttctg cacaacaatt
     1021 ttgcaaaaaa aagcgatcat tcataactta aacgtctttc agtcactccg ggatttccca
     1081 tcatcgcaga ctgaaagact ctaactggag cgggcagcgg gaatcgaacc cgcatcatca
     1141 gcttggaagg ctgaggtaat agccattata cgatgcccgc atatggtgcc gactaccgga
     1201 atcgaactgg tgacctactg attacaagtc agttgctcta cctactgagc taagtcggcg
     1261 ctggttcctc gggtgtgaat gagatgcccg gatacataat tgacccttcc ctcggaacca
     1321 aagaaatcat actgataaat aaacagagtt caatgtctgc ttattcatct gttgaaaaaa
     1381 cactcagaat tacagagcga tattcacact caccacgatt ctgacatacc gcccgatttt
     1441 cgaagcgtga gggacacgca agcagtacat gtccggtatt tcagcatccc catcagttaa
     1501 catttaaagc acataacaat gtatacgagc atcctaattg atgcttcttt tatggaacgt
     1561 aggtattgcc ttaagggtaa accggcaagt gctgagttat ttgagcaaag taaagtgatg
     1621 atcttcaccc aactacagac caaaaagggg cgtgaacgcc cctgtattta tatgacttta
     1681 acgaaaacac tgaccaaatt taatcagcat ccacaacact aataatatct gcgtccagaa
     1741 acaaaagacg cgcataaata aaccgtagat tctcagttaa acttcacctg ggcaaagcct
     1801 gagcctgaac tgcaggtatt agatatgatt gttacagtca tccctgtcag ctgagcactt
     1861 tgtaacaatg gctgcaaatt ccatctgttc gtccagtatt ctcttcctga caccttcaca
     1921 gtaaaggtat catcctcatt atacttggaa aactcaattt ttcctacagc acaatccgcc
     1981 gccatggaat tagcagaaaa gagaccgaat aaaactgcaa taatcatctt cttcatattc
     2041 accccctgtt attggtttgg ttcagtaaga tcctgaggct tgcgatttaa taaagcagcg
     2101 attgtattcg cttcccacaa aacattattt actttaatgg ccgccctgtc tccaacaatc
     2161 tggcattctg ttttctgttt ttggctcacg gaacgaactg aataacttcc ggttgagtgg
     2221 caattaagga tgaccgcaac acttccgaga atcgcagaaa gactattaaa agatatccta
     2281 ccgattctta ccccttcctc tcctctgtac tctggaagaa cattacttat tcttccccag
     2341 ttcagggtaa ggtcaacatc ctgagccgtc attgtataaa gcggagatgc ctcagacagc
     2401 gccggtcgga atccccgctg tatttgtctg aatcgcagag cttctgctat cactgtgaca
     2461 aaacgcagca tagctctgga tgatgcgcgg gtcatggaac gtcctctgaa ctccattaaa
     2521 tccagatatg aaccaaccag tgaatgacgc ccaatctgca tccctgtacg ttccagatct
     2581 gcgattcgct gtaatgatga ataactgctg tccgtcgtca tggaaacagt tatcacatca
     2641 ggcactgaaa tatgtgagaa atcggagaat ctgtaaaagg tattcgtttc agtattaata
     2701 aagcctgcaa catataagtt attccgttcc attattaaac ggaggtggtt aaatctctcg
     2761 ctatatggat caaggcctct aacattcaat gatatatagt ttccgcctgg aacatgatta
     2821 attactgaaa cagaaacacc accctgagat atatttccaa gtggagtgga tattgccgac
     2881 cttatactat tcaatgaatc aacatagctc ttttgcgaag agaaatccac agtaaactca
     2941 tctgcataag atgctgaaga caagcaaaca caaaaaaaca acaccagctt taataatata
     3001 tatcgcatat attaacactc catcagtctg aaccggcagc gccaggaata atacctcgca
     3061 agaagtccgg aatgttttta gcagaaattt attacaaaca aatcccctca caggaaatga
     3121 ctgcacaatg tattacttcc ctgtttatca ggaacagaaa gcagataatg atgcactata
     3181 gcttccggaa aatagtatca attatttaag attacgggta tatggtttat atttttatag
     3241 aatagagaat ctctcacata taaagaacac cataaacttt acttcagcca cagcctggtc
     3301 aagaggagcg tccattgact atgccccccc ctggcaccac gaccggttta cggacataaa
     3361 aaaaccgccc ggcggcgtgt ttaagctgtg tggcgaagtt acccatcttt accaagtatt
     3421 cggtaataaa ataacttgtg gtgttctcat ttttatgccg ataaaataaa cggtccactt
     3481 tcaaatggag cgattcatga tttttctcag aactgaaaac ggcatcgaga aacttgaaaa
     3541 ctgggaggaa attatatcca gaccaaactt tgtcaccact atagacaaag gagttcaaca
     3601 actt
//
LOCUS       DAZFJL010000227         3518 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFJL010000227 DAZFJL010000000
VERSION     DAZFJL010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.229"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..232)
                     /gene="escO"
                     /locus_tag="ACHJ8C_004553"
     CDS             complement(<1..232)
                     /gene="escO"
                     /locus_tag="ACHJ8C_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase activity
                     positive regulator EscO"
                     /protein_id="HFO5554265.1"
                     /translation="MAKINSQIKEVDGKLDDCEQSIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRSLLD"
     gene            complement(292..1632)
                     /gene="escN"
                     /locus_tag="ACHJ8C_004554"
     CDS             complement(292..1632)
                     /gene="escN"
                     /locus_tag="ACHJ8C_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFO5554266.1"
                     /translation="MISEHDSVLEKYPRIQKVLNSTVPALSLNSSTRYEGKIINIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHVSGMYCGQWLSYQGDEF
                     KIRVGDALLGRLVDGIGRPMESNIVAPYLPFERSLYAEPPDPLLRQVIDQPFILGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEYFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYKN
                     VELLIRIGEYTMGQDPEADKAIKNRKLIQNFIQQSTKDISSYEKTIESLFKVVA"
     gene            complement(1616..>3518)
                     /gene="escV"
                     /locus_tag="ACHJ8C_004555"
     CDS             complement(1616..>3518)
                     /gene="escV"
                     /locus_tag="ACHJ8C_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFO5554267.1"
                     /translation="IAINISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVS
                     TTRLILLHHDAGDIIYSFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSAR
                     FSLDGMPGKQMSIDGDMRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAG
                     IIIVLVNLFGGVLIGMWQFDMPFSEALSLFSVLSVGDALVAQIPALIISVTAGVVVTR
                     VPGESEKEENLAGDIVQQVSVNSRPFLISAALMLVMAIIPGFPTLVFLFLAVCLLGIA
                     WKLQKKRTFGTGNNKDAMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWN
                     FFEESGIPLPKIIVNPVKNNDSAIEFLLYQESIYKDTLIDDTVYFEAGHAEISFEFVQ
                     EKLSTNSIVYKTNKANQQLAHLTGMDVYATTNDKITFLLKKLVLSNAKEFIGVQETRY
                     LMDIMERKYNELVKELQRQLGLSKIVDILQRLVEENVSIRDLRTIFETLIFWSTKEKD
                     VVILCEYVRIALRRHILGRYSVSGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPE
                     RTEQIIGFLKNIMNPTGNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKV
                     LGTVNDFRA"
BASE COUNT         1063 a          837 c          645 g          973 t
ORIGIN      
        1 aatctagcaa tgaacgcttt tccttcgaaa gtgaagattt tttctcatac aatcgctgct
       61 tttgctcatc aaatgcattg tttttaatct gatatttata caacgacact ttatccaaat
      121 tcaccagcga tgcacaatat gcctgcttag aggcaatcga ctctttaatg gattgctcgc
      181 agtcatcgag tttaccatcc acctctttta tctgagagtt aatcttagcc attgattctc
      241 tcaatcggtt cgctctgctt ttacgaatag ataaaattct gtccaacata ctcaggcaac
      301 cactttgaat aggctttcaa tcgttttttc gtaactactg atatctttgg tgctttgttg
      361 tataaagttc tgaattaatt tcctattttt tattgcctta tctgcttcag gatcttgccc
      421 catcgtgtac tcaccaatac gaataagcag ctcaacattt ttataagttg caataagctt
      481 tttgcattct gccgccgcac gcaaatgctc cgatgtaaca acgttatgca taacacgact
      541 ggcacttaaa ccaatatcaa ttgcagggaa atggttttcc tctgcaagtt ctcgggtaag
      601 tacgatgtgc ccatcaagaa tagagcggac ttcatcgcca ataggatcat taacattatc
      661 gctttctaac aacaccgtat aaatcgcggt tattgaacct tttggcgcag gccccgctcg
      721 ctctaataat ttgggtaacg aagaaaaaac actcggaggg aatccccccc ttacatcagg
      781 ctctcccgat gcaagcccaa catcacgcgc ggctcgggca taacgagtta cagaatccat
      841 cattaataat acatttttac cttgatcgcg gaagtactct gcaatagtcg ttgccgtaaa
      901 tgcggctttc atcctttcca gcgcggggcg gtctgacgtt gtgacgacca gcacacattt
      961 agaaagcgtg gattgaggta aaagcgcgag gaattcattt acttcgcgac cacgttcgcc
     1021 gataagagca aggacaataa tatctgcgga tgcgccatta caaatcatcc ccaaaagcgt
     1081 acttttacca acgcctgaac cagcaaaaat accgatacgc tgcccaatac cgcaagttag
     1141 tagcccatca attgccctga cgccaagtat aaatggctga tcaataacct gccttaataa
     1201 aggatctggt ggttcagcat acaagctgcg ttcaaacgga agataggggg caacaatatt
     1261 actctccatt ggtctgccta tgccatcaac aagccgccca agtagtgcgt ctccaacacg
     1321 aatcttgaac tcatccccct gataacttaa ccactgacca cagtacatgc cggaaacatg
     1381 ctcaaaaggc aacaagaaaa cctcatcttc atcaatagcg atcacctcgg ctaaacgttg
     1441 actgggttct atcttgtaaa aggcgccaat acgcgctttt ggtaaacgcg ccttaataat
     1501 cgtcccgccg atattgataa tcttgccttc atatctggta gacgaattta atgataatgc
     1561 cggcacagtg ctattgagca ctttctgaat acgtgggtat ttttccaata cagaatcatg
     1621 ctctgaaatc atttaccgtt cctaatactt taagttcgat attattccca acctcctgaa
     1681 aagagagcac ggggactgac gggaacgaac cttcaatcat tttcttcaca tagcgcctga
     1741 tatctaaagc ggtcagaatg acgccgttcc ccgttggatt catgatattt tttaagaagc
     1801 caattatctg ctcagttcgc tccggtgaga tattcagata cgaacctgat gacgtttgtc
     1861 tgatcgactc tcgtagctca ttttcaatat cagagccaat aagccaaacg ttcagaagtg
     1921 taccgctaac gctatagcga cctaaaatat gccgacgcag ggcgatacga acgtattcgc
     1981 acaagataac cacatccttt tcttttgttg accaaaaaat aagcgtctca aagatagttc
     2041 tcaggtctct aattgagaca ttttcctcta cgagacgttg taggatgtca acaattttgc
     2101 tcaaaccaag ctggcgctgc agctctttca caagctcgtt atattttctc tccatgatgt
     2161 ccatcaaata acgcgtttct tgtacgccga tgaactcttt ggcattagac aatacaagtt
     2221 tctttagcaa aaacgttatc ttatcatttg ttgttgcata aacatccata cctgtaaggt
     2281 gagcgagctg ttgattagcc ttattcgtct tatatacgat agagttagtc gaaagctttt
     2341 cctggacaaa ttcgaatgat atctctgcat gcccagcctc aaaatagaca gtatcatcta
     2401 taagagtatc tttgtatatt gactcttgat agagcaaaaa ttctattgcg ctatcattat
     2461 ttttaaccgg attaacaata atcttaggca atggaattcc actttcctca aagaaattcc
     2521 aacgcatgac ctcaatttgc tgtgtaatgt ctgaactata aatattacta cttaagttta
     2581 aaatcaatgg ctcagcgccc ggtgagatgt tttggctatt agacaaatca gctcccatag
     2641 catccttatt attgccagtt ccaaatgttc ttttcttctg taatttccag gctatcccca
     2701 acaggcaaac cgccagaaat aagaagacca atgtaggaaa gcccggaata atcgccatta
     2761 cgagcattag tgcggcactg attaaaaaag ggcgactatt aacagaaacc tgttgaacaa
     2821 tatcacctgc aaggttttct tctttttcgc tttcaccggg cacacgagta acaaccacgc
     2881 ccgcggtgac agaaataata agcgcaggga tctgggcaac caaagcatca ccgacagaca
     2941 atacagaaaa caggctaagt gcctcactaa atggcatgtc aaattgccac ataccaatga
     3001 gcacgccacc aaaaaggtta actaaaacaa taatgatacc agcaatagcg tcccctttta
     3061 caaacttcat cgccccgtcc attgaaccat aaaactggct ttccttttgc actcgggagc
     3121 gaagaacttt tgcctcaagc gggtcaataa cgcctgcgcg catatcacca tcaatactca
     3181 tttgctttcc tggcatacca tcaagggaaa aacgagcact cacttcagca acacgctcgg
     3241 ccccttttgt aataaccata aattgaacaa tggtaataat agtaaaaata accaggccaa
     3301 caacaatatt accgccaaca acaaagttac cgaaggagta aataatgtca cccgcatcgt
     3361 gatgcagcag aataagtctg gttgtactaa cactgagcga caggcgcatc aacgtcgtaa
     3421 tcaacaggat tgtggggaaa gaagttagtt caagaggatt ttttatatag attgagagca
     3481 ttaataaaag taaagctgtc gaaatattaa tcgcgata
//
LOCUS       DAZFJL010000228         3461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFJL010000228 DAZFJL010000000
VERSION     DAZFJL010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.230"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..391)
                     /gene="cesD2"
                     /locus_tag="ACHJ8C_004556"
     CDS             complement(<1..391)
                     /gene="cesD2"
                     /locus_tag="ACHJ8C_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HFO5554268.1"
                     /translation="MVDTFNDEVFNYYLEQKGYTIQKEFLCGSAFFIGWRIETPFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTRLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFV"
     gene            complement(398..1363)
                     /gene="espB"
                     /locus_tag="ACHJ8C_004557"
     CDS             complement(398..1363)
                     /gene="espB"
                     /locus_tag="ACHJ8C_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFO5554269.1"
                     /translation="MNTIDNNNAAIAVNSVLSSTTDSTSSTTTSTSSISSSLLTDGRV
                     DISKLLLEVQKLLREMVTTLQDYLQKQLAQSYDIQKAVFESQNKAIDEKKAGATAALI
                     GGAISSVLGILGSFAAINSATKGASDVAQQAASTSAKSIGTVSEASTKALAKASEGIA
                     DAADDAAGAMQQTIATAAKAASRTSGITDDVATSAQKASQVAEEAADAAQELAQKAGL
                     LSRFTAAAGRISGSTPFIVVTSLAEGTKTLPTTISESVKSNHDINEQRAKSVENLQAS
                     NLDTYKQDVRRAQDDISSRLRDMTTTARDLTDLINRMGQAARLAG"
     gene            complement(1384..2526)
                     /gene="espD"
                     /locus_tag="ACHJ8C_004558"
     CDS             complement(1384..2526)
                     /gene="espD"
                     /locus_tag="ACHJ8C_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspD"
                     /protein_id="HFO5554270.1"
                     /translation="MLNVNNDIQSVRSGASAATATSGINQSEVTSALDLQLVKSTAPS
                     ASWTESTALPTPPAGHSLVTPSAAEDVLSKLFGGISGEVTSRTEGTESQRSTQNASSG
                     YPYLSQVNNVDPQAMMMMATLLSLDASAQRVASMKNSNEIYADGQNKALDNKTLEFKK
                     QLEEQQKAEEKAQKSKIVGQVFGWLGVAATAIAAIFNPALWAVVAISATAMALQTAVD
                     VMGDDAPQALKTAAQAFGGLSLAAGILTAGIGGVSSLISKVGDVANKVGSNIVKVVTT
                     LADTFVDNVASKISAVANGLTTSSRSIGTTVLNNDAAYYNVLSQVSAFAVENLTRQSE
                     YLSQSAKAELEKATLELQNQANYIQSASQLMSDSARVNIRIVSGRV"
     gene            complement(2539..3117)
                     /gene="espA"
                     /locus_tag="ACHJ8C_004559"
     CDS             complement(2539..3117)
                     /gene="espA"
                     /locus_tag="ACHJ8C_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFO5554271.1"
                     /translation="MDTSTTASVASANASTSTSMAYDLGSMSKDDVIDLFNKLGVFQA
                     AILMFAYMYQAQSDLSIAKFADMNEASKESTTAQKMANLVDAKIADVQSSSDKNAKAQ
                     LPDEVISYINDPRNDITISGIDNINAQLGAGDLQTVKAAISATANNLTTTVNNSQLEI
                     QQMSNTLNLLTSARSDMQSLQYRTISGISLGK"
     gene            complement(3174..>3461)
                     /gene="sepL"
                     /locus_tag="ACHJ8C_004560"
     CDS             complement(3174..>3461)
                     /gene="sepL"
                     /locus_tag="ACHJ8C_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE gatekeeper SepL"
                     /protein_id="HFO5554272.1"
                     /translation="SLNNYTDIIAISIKKDKDVILNEVLSIIEHVWLTEDWLLESPSR
                     VSIVEDKHVYYFHLLKEFFASLPDACFIDNEQRSNTLLMIGKVIDYKEDVM"
BASE COUNT          976 a          797 c          669 g         1019 t
ORIGIN      
        1 ttacgaacca aattccgtct tctgtttctt gctgcacggc accctttctg atgaagtaat
       61 taaacaactc ttcgcgcttt tgacgttctg aatctgaacc aatcttagat ttcatcgcac
      121 tgattttctt aatacccgaa aagtggtgat acaactcttc gagcaaacga gtcagtgata
      181 aaacagggcc gttaagccct gtttggttac gtgcttcaaa agagcacaaa atcagttctt
      241 gttcatccag tctgtacgct aatgaaaaaa aaggtgtctc aatccgccat ccgataaaaa
      301 aggcactgcc acaaagaaac tccttctgta ttgtgtaccc tttttgttca agatagtaat
      361 taaacacttc atcattaaac gtatcgacca tgatcaatta cccagctaag cgagccgctt
      421 gccccatgcg attaataagg tcagtaagat cacgggctgt cgttgtcata tcacgtagac
      481 ggctagagat atcatcctgc gctctgcgaa catcctgttt gtaggtatct aaattcgacg
      541 cctgaagatt ttctacagat ttagcacgct gctcattgat atcatgattg gacttgacag
      601 attcagatat cgttgttggc aatgttttcg taccttcagc aaggctggta acaacaataa
      661 atggcgttga accggaaatc cttccggcag cagcagtaaa gcgacttaat aatcctgcct
      721 tctgtgctaa ttcttgagca gcatcagcag cctcttcagc tacctgagaa gctttctgag
      781 ccgaagtagc aacatcatca gtgataccgg atgtacgact ggccgctttt gcagctgtcg
      841 cgatagtttg ctgcattgcg ccagctgcat catctgctgc atctgcaata ccttcggaag
      901 ccttcgccag tgctttagtt gaagcttcag agactgtacc gatagacttc gcagaagtag
      961 aagcggcttg ctgagcgaca tcactcgcgc ctttagttgc gctattaatg gcagcaaaag
     1021 agcctaaaat ccccaggaca gatgagatag caccaccaat cagagcggct gtcgcaccgg
     1081 cttttttctc atcaatagct ttattttggc tctcaaaaac ggctttttgg atgtcatagc
     1141 tttgcgccaa ttgcttctga agataatctt gcaatgtggt gaccatctca cgcaacagtt
     1201 tttgaacctc cagcagaagt ttagaaatat ccactctgcc atcagtaagt aaagatgaac
     1261 tgattgacga agttgatgta gttgttgaac tggtgctgtc agtcgtgctg ctcaaaacag
     1321 aattaactgc gattgccgca ttgttattat cgatagtatt cataataaac gcctctttaa
     1381 taattaaact cgaccgctga caatacggat attcactcgc gcagaatcag acatcagctg
     1441 agaagcactc tgtatataat tcgcctggtt ttgcagctcg agagtagctt tttccagctc
     1501 tgccttcgca ctctgactta agtactcact ttgtcgagtt aaattttcca cagcaaaagc
     1561 agaaacctgt gataacacat tataatacgc tgcgtcatta ttcagcacag ttgtaccaat
     1621 agagcgcgaa gaagtagtta aaccatttgc cactgccgaa attttcgatg caacattatc
     1681 aacaaaagtg tcggccagcg tagtgactac ttttacgatg tttgaaccaa ctttgttagc
     1741 gacatcgcca actttagata taagtgaaga caccccgcca atgccggctg tcagaatacc
     1801 tgcggcaaga gatagaccgc caaaagcttg cgctgctgtt tttaacgcct gtggagcatc
     1861 atcgcccata acatcaactg cagtttgcag tgccattgct gttgcactaa tagcaacaac
     1921 agcccagagt gccgggttaa aaatagctgc aatagcagtt gctgcaaccc ctaaccaacc
     1981 aaagacctga ccaacaattt tacttttttg tgctttctct tcggcttttt gttgttcttc
     2041 gagctgcttt ttaaactcca gcgttttatt atccagcgct ttattttgtc catccgcgta
     2101 gatctcgttg gagtttttca tactcgcaac tctctgcgct gaggcatcca gggataacag
     2161 agtggccatc atcatcatgg cctgagggtc aacgttattc acctgagaaa gataaggata
     2221 gccagagctt gcattctgag tgctgcgctg tgattctgtt ccctctgttc gactcgtaac
     2281 ttcaccacta ataccaccaa acagcttact caggacatcc tcagcagcag agggcgtcac
     2341 taatgagtga cctgccggtg gcgttggtaa agcggtactt tctgtccaac ttgctgatgg
     2401 ggccgtggat ttaaccagtt gtaaatccag cgcggaagtc acttcagact gattaatacc
     2461 tgaagtagcc gtagcagcac tggctccaga cctcacagac tggatatcgt tatttacatt
     2521 aagcatagtt atctccggtt atttaccaag ggatattcct gaaatagttc tatattgcag
     2581 tgactgcata tcagaacgtg cactcgttaa taggtttaac gtatttgaca tttgctgtat
     2641 ttcaagctgg ctattattga ccgtcgttgt caaattattc gctgtagctg aaatagctgc
     2701 tttcaccgtt tgcaaatcac cagcgcctaa ttgagcattt atattgtcaa taccacttat
     2761 tgtaatgtca ttgcgaggat catttatata tgaaatcact tcatcaggaa gttgagcttt
     2821 cgcattcttg tcagagctac tctgaacgtc agcaatttta gcatctacaa gattagccat
     2881 tttttgggca gtggttgact ccttagatgc ctcattcata tcagcaaact ttgcaatcga
     2941 cagatcgctt tgtgcctgat acatataggc aaacatgaga attgcagcct gaaaaacacc
     3001 gagtttatta aatagatcaa taacgtcatc tttcgacatg ctccctaaat cataggccat
     3061 tgatgtcgaa gtactcgcat tcgcactagc aactgatgct gtagttgatg tatccatgta
     3121 tatacctctt cataatcttt cttagcatga caaaaactat aaaaaaaaca atatcacata
     3181 acatcctcct tataatctat cactttacca atcattaata atgtattact cctctgctcg
     3241 ttatcaataa aacaagcatc aggtaatgat gcaaaaaatt ctttcaataa atggaaataa
     3301 taaacatgtt tatcttcgac aatcgatacc cgagaagggc tctccagtag ccagtcttct
     3361 gttaaccata catgttcaat aatagatagc acttcattta aaatgacatc tttatctttc
     3421 ttaatggaga ttgcaataat atccgtatag ttattaagtg a
//
LOCUS       DAZFJL010000229         3441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFJL010000229 DAZFJL010000000
VERSION     DAZFJL010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.231"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            31..597
                     /gene="yceJ"
                     /locus_tag="ACHJ8C_004561"
     CDS             31..597
                     /gene="yceJ"
                     /locus_tag="ACHJ8C_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO5554273.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLQSYSPMTRLGAKAGHLTLYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHVIDKDDTLKRMLGKSSSDYGV"
     gene            601..1176
                     /gene="yceI"
                     /locus_tag="ACHJ8C_004562"
     CDS             601..1176
                     /gene="yceI"
                     /locus_tag="ACHJ8C_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO5554274.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1218..2270)
                     /gene="trhO"
                     /locus_tag="ACHJ8C_004563"
     CDS             complement(1218..2270)
                     /gene="trhO"
                     /locus_tag="ACHJ8C_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO5554275.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2495..3415
                     /gene="lpxL"
                     /locus_tag="ACHJ8C_004564"
     CDS             2495..3415
                     /gene="lpxL"
                     /locus_tag="ACHJ8C_004564"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO5554276.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
BASE COUNT          857 a          856 c          841 g          887 t
ORIGIN      
        1 ttctctcgca aattcgtttt ggatgcactt atgtcattca caaatacccc tgaacgctat
       61 ggtgttatat ctgcggcctt ccattggcta agcgcaatta ttgtctacgg catgtttgct
      121 ttaggtctgt ggatggtcac gctcagttat tacgatggct ggtatcacaa agcacccgaa
      181 ctgcataaaa gcatcggtat tttgttaatg atggggctgg ttattcgcgt tctgtggcgc
      241 gtcatatctc caccgccggg tccgctgcag agttattcgc ccatgactcg tcttggcgca
      301 aaggctggac atctcacatt gtacttattg ctgttcgcca tcggcatcag cggctatctg
      361 atctcaactg ccgatggtaa accgatcagc gtttttggct ggtttgacgt ccccgcgacc
      421 ctcgccgacg ctggcgcaca ggctgacttt gccggtgccc tgcatttttg gctcgcgtgg
      481 agcgtcgtag tactgtccgt tatgcacgga tttatggccc tgaagcatca tgtcatcgat
      541 aaagacgaca ctctgaagcg catgctggga aagtcgtcat ctgactatgg agtataaaaa
      601 atgaaaaaaa gcctgcttgg tttaaccttc gcttccctga tgttctctgc cggttcagcg
      661 gttgccgccg attacaaaat tgacaaagaa ggtcagcacg cctttgttaa cttccgcatc
      721 cagcatcttg gctatagctg gttatatggc accttcaaag atttcgacgg tacttttacc
      781 tttgatgaaa aaaatccagc tgccgataaa gtgaatgtga caattaacac caccagcgtc
      841 gatactaatc acgccgaacg cgataaacat cttcgcagtg cggatttcct caataccgca
      901 aaatatccac aggcaacatt cacctccacc agcgtgaaga aagacggtga cgaactggat
      961 attaccggcg atctgacgct gaatggcgta accaaacccg tcacgctgga agcgaaatta
     1021 attggtcagg gcgacgaccc atggggtggt aaacgtgcag gcttcgaggc cgaaggcaaa
     1081 attaagctca aggacttcaa tatcaagaca gatttaggtc cagcttctca ggaagtggat
     1141 ctgattattt cagtggaagg cgtacagcag aagtaagagt gaaatgttgc cggatggcac
     1201 gcatccggca atgatattta ttctgttgga tcaggaatgc ccagtgttgt attcagacgt
     1261 ccacgagact tattaaagat cttattgcca ttttcacgtc ctgcccgacg gcgtcgctgt
     1321 tcttctggcg gtaacgcgct ttcttcgcag caaatttcac tacaacaacc tttgtatttt
     1381 tctgcacata ctggacactg aataaacagc aggtggcagc catcattttt acagttggta
     1441 tggctgtcgc acggcgcacc gcactggtgg caatgcgcga taatctcatc agatatacgt
     1501 tcgcccatcc gctcgtcaaa aacaaaattt ttgccaataa aacgtaccgg taaaccctgc
     1561 tcgcgcgcct tgcgggcgta ttcaataatt ccgccctcga tatgccagac tttattgaat
     1621 ccgttatgtt tcatccaggc actggccttt tcacaacgaa tgccgccggt gcagtacata
     1681 acgatttttt tatctttatg tgcctgcatc atctcgactg cttttggcag ctgctcacgg
     1741 aaggtatctg ccggaatttc cagcgcgttt tcaaagtgcc ccacttcata ctcatagtgg
     1801 ttacgcatgt cgataaacag tgcatcggga tcgtcaagca tggcgttcac ttccgccgct
     1861 tgcaaatact cacccacatt actggcatcg aagtgaggat catcaatacc gtcggcaaca
     1921 atgcgatcgc gtaccttcat gcgcagcacc cagaaggatt tcccgtcatc atccaatgcg
     1981 atattcaggc gtaagccctc taaagccggg tcgaaggcat aaagctgcgc gcgaaatgtt
     2041 tcaacattgc tcgcaggtac gctgatttgc gcgttaatgc cctcatgcgc cagatacact
     2101 cgcccaaaaa cattcagcgc ggtaaacagc tgatataaag cgtcacgggt cgccttagga
     2161 tcggcgatgt ggaaatactt ataaaacgaa atggtggttc gcggttcgct ctcagccaac
     2221 attttggctt ttaacgcgtc gttggaaatg cggttgtgta acactggcat ggtgtacggt
     2281 tcctgcgaga tgggaaagta aaaatccgcg gcatgatata gcaattatcg ataattaaca
     2341 tccacacatt ttacgctaca tttgcgcatt aaaaattatt tgttatttac aagcgcggca
     2401 atttcgccca gtcttcagtc acaaattttg gttgcggacg aaaaaatgcg acaatacata
     2461 caattgcccg tataggttga aaaacaggat tgatatgacg aatctaccca agttctccac
     2521 cgcactgctt catccgcgtt attggttaac ctggttgggt attggcgtac tttggttagt
     2581 cgtgcaattg ccctacccgg ttatctaccg cctcggttgt ggattaggaa aacttgcgtt
     2641 acgttttatg aaacgacgcg caaaaattgt gcatcgcaac ctggaactgt gcttcccgga
     2701 aatgagcgaa caagaacgcc gtaaaatggt ggtgaagaat ttcgaatccg ttggcatggg
     2761 actgatggaa actggcatgg cgtggttctg gccggaccgc agaatcgccc gctggacgga
     2821 agtgatcggc atggaacaca ttcgtgacgt gcaggcgcaa aaacgcggca tcctgttagt
     2881 tggcatccat tttctgacac tggagcttgg tgcgcggcag tttggtatgc aggaaccggg
     2941 gattggcgtt taccgcccga acgataatcc actgattgac tggctacaaa cctggggccg
     3001 tttgcgctca aataaatcga tgctcgaccg caaagattta aaaggcatga ttaaagccct
     3061 gaaaaaaggc gaagtggtct ggtacgcacc ggatcatgat tacggcccgc gctcaagcgt
     3121 tttcgtcccg ttgtttgccg ttgagcaggc tgcgaccacg accggaacct ggatgctggc
     3181 acggatgtcc ggcgcatgtc tggtgccctt cgttccacgc cgtaagccag atggcaaagg
     3241 gtatcagttg attatgctgc cgccagagtg ttctccgccg ctggatgatg ccgaaactac
     3301 cgccgcgtgg atgaacaaag tggtcgaaaa atgcatcatg atggcaccag agcagtatat
     3361 gtggttacac cgtcgcttta aaacacgccc ggaaggcgtt ccttcacgct attaaatctc
     3421 ccatgccgga tgcttcagaa g
//
LOCUS       DAZFJL010000230         3357 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZFJL010000230 DAZFJL010000000
VERSION     DAZFJL010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.232"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            109..1659
                     /locus_tag="ACHJ8C_004565"
     CDS             109..1659
                     /locus_tag="ACHJ8C_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO5554277.1"
                     /translation="MKTVFTTLSVIFGVIFSHSVLAQDVTIYYTNDIHAHVNPAKILA
                     VDKNRLIGGMANIAGIVNEAKKKSKDVFFFDAGDYFTGPYISTLTKGEAIIDIMNTMP
                     FDAVSVGNHEFDHGVPNMVSQLSKAKFPILLGNIYYTDTNKPVWDHPWTIIEKDGLKI
                     GVIGLHGAFAFYDTVAAKAREGVEARDEIKYLNKALAELKGKVDITVLLIHEGVPARQ
                     SSFGSKDVERLLQADIETAKKVNGVDVLITGHAHVGTPQPIKVNNTLIVSTDAYGTNI
                     GKFVLDYNPKTKKIDSYNGELITIFADQFKPDTIVQNTIDKWSAKLNKITQEVVGHSP
                     VVLTREYGSSSSTGNLILDAMMEKTPDAIAGFQNSGGMRADFPKGDITLGDVISTFPF
                     NNDLIEMDLTGRDLKSLMTHATNLTNGVLQVSKSVAVVYDSKKPLNQRLISFTINGKP
                     VEDNQTYRIATHSFCASGGDGFEAFLNGKNVKTIPGTTSAESIIDYFKNHKPVTPDLT
                     KRVMDVAK"
     gene            1700..3319
                     /locus_tag="ACHJ8C_004566"
     CDS             1700..3319
                     /locus_tag="ACHJ8C_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO5554278.1"
                     /translation="MKTAQRTLLAATLLTIFSTSVMAQDVTIIYTNDLHAHVDSYKVP
                     YVADGKRDIGGFANISTLVKQEKAKNKATFYFDAGDYFTGPYISSLTKGEAIIDIMNT
                     MPFDAVSIGNHEFDHGWDNALRQLSKANFPVLLGNVYHKESEKPFWNKPYTILEKDGI
                     KIGIVGLHGVFAFNDTVSELSLQGLDNDNNNRFDKSSATLKNQGIEAREEVKYLQHYI
                     DELRDKVDLTVALVHEGVPARQSSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVG
                     TPEPIKVGNTLILSTDSGGIDIGKLILDVNPTARTHKVKSFELKTVYADEWIPDPTTQ
                     KVINGWNKKLADLVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSG
                     SLRADINAGTITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQMSKGAE
                     MRYTLQKPVGQRIVSFKINGEEIVDTNIYHVATTTFLALGGDGFLAFKEGKNVQVRAG
                     NNMSNVVIDYFKKGHKITPAQVNEMRVEVSK"
BASE COUNT         1051 a          703 c          714 g          889 t
ORIGIN      
        1 ctaagatcac acaatacttt tctgaactat gcttacataa aataagacac gatgtgcctt
       61 ttatttaaat aattcaacaa atgtaacaca ccctatccag gagggatgat gaaaactgta
      121 tttacaacat tatcagtcat attcggcgtg atattttctc acagtgtcct agctcaggac
      181 gttactattt attatacgaa tgacattcac gcacatgtca atccggcaaa aatcctcgct
      241 gttgataaga acagacttat aggtggtatg gctaatattg caggcatcgt taatgaagcg
      301 aagaaaaaaa gcaaagatgt gtttttcttt gatgcaggcg actattttac cgggccgtat
      361 atcagcaccc tgaccaaagg tgaggctatt attgatatca tgaacactat gccctttgat
      421 gcggtgtctg ttggtaatca cgagttcgat cacggtgtgc ccaatatggt gtctcagtta
      481 tcaaaagcaa aattccccat tctgctggga aatatctact acaccgatac gaataaacca
      541 gtttgggatc atccgtggac cattatcgaa aaagatggtt taaagattgg tgtgattgga
      601 ttgcatggcg cattcgcttt ctatgataca gtcgcggcaa aagcacgtga aggcgtagaa
      661 gccagagacg aaattaaata tttaaataaa gcacttgcag aattaaaagg taaagtcgac
      721 attaccgttt tgctaattca cgaaggtgtc ccggcgcgtc aatcaagctt tggtagtaaa
      781 gatgtggagc ggctactaca ggcagatatt gagacagcta aaaaagtgaa tggtgttgat
      841 gtcttaatta ccggtcacgc acatgtcgga acaccgcaac caataaaagt taataacaca
      901 ttaattgtat ctaccgatgc atacggcacc aacatcggaa aatttgtgct tgattacaat
      961 ccgaaaacga agaaaattga tagttataat ggtgagttaa tcaccatctt tgcagatcaa
     1021 tttaaacctg acaccatcgt tcaaaatact attgataaat ggagtgcaaa gcttaacaaa
     1081 ataacacagg aagttgtcgg ccattctccc gtagttttaa cacgtgagta tggtagttcc
     1141 tcttctaccg gcaaccttat tcttgatgca atgatggaaa aaacacctga tgccattgcc
     1201 ggatttcaaa atagcggtgg gatgcgagct gattttccta aaggcgatat cacactggga
     1261 gatgttatta gcacattccc ctttaataat gacctcatcg agatggattt gacgggccgc
     1321 gatctcaaat cattgatgac gcatgcaacc aatctaacta acggtgtgtt acaggtttca
     1381 aaaagcgttg cggttgtcta tgacagcaaa aaacccctca accaacggtt aatctctttc
     1441 accattaacg gcaaacccgt ggaagataat caaacatatc gtattgccac gcactccttt
     1501 tgtgccagtg gtggtgacgg ttttgaagca tttttgaatg gaaaaaatgt gaagacgata
     1561 ccgggaacaa cctcggcgga atctatcatc gattatttca aaaatcataa acctgtcacc
     1621 ccagacttaa ctaaacgagt catggacgtc gccaaataaa atctcattta tcagtcaatt
     1681 tatttaacaa ggaatgcgaa tgaaaacagc gcagcgtact ttacttgccg caacattgct
     1741 gactattttt tcgacctcag tcatggcgca ggatgtcacg attatttata ccaatgatct
     1801 tcacgctcat gtggattcct ataaagtacc gtatgtcgca gacggaaaac gcgacattgg
     1861 tggctttgcc aatatatcca cgttagtaaa acaagagaaa gccaaaaata aagccacctt
     1921 ttattttgat gctggagact atttcaccgg gccatatatc agtagcctga caaagggcga
     1981 agcaataatt gatattatga ataccatgcc ttttgacgca gtatcaatcg gtaatcatga
     2041 gtttgaccat ggctgggata atgccctccg acaattaagc aaagcgaatt tccctgtttt
     2101 attaggcaat gtttatcata aagagagcga gaaacccttc tggaacaagc cctacaccat
     2161 cctggaaaaa gatggcatca agattggtat tgttgggcta cacggcgtat ttgcatttaa
     2221 tgacacggtc tcggagctct ctctccaggg actcgataat gacaataata accgatttga
     2281 taaatcttcc gcgaccctca agaatcaggg gattgaagca cgtgaggaag tgaagtattt
     2341 gcagcattat atcgacgagt tacgtgataa ggttgacctc accgtggcgc tggttcatga
     2401 aggcgtaccg gcgcgtcagt ccagcattgg caatacagat gttagacgtg cgctggataa
     2461 agatattcaa accgcaagca aggtgaaagg attggatatt ctgattaccg ggcatgccca
     2521 cgtcggaacg ccagaaccaa tcaaagtcgg caacacctta atcctttcga ctgacagtgg
     2581 cggtatcgat attggtaaat taatccttga tgtcaaccct acagctcgta ctcataaagt
     2641 gaagagcttt gagctgaaaa cggtttatgc cgatgaatgg atacccgatc caaccacaca
     2701 aaaagttatc aatggctgga ataaaaaact ggcggatctt gtacgccaac cagtcggtga
     2761 atcatcgatt gcattaactc gtgcctacgg tgagtcatcg caacttggca acctgtttac
     2821 cgatgcaatg cttgtcgctg caccgactgc acaaattgcg ttgataaact ccggcagttt
     2881 acgtgctgat ataaatgcag gcacgattac ctttggtgac attaccagta cattcccctt
     2941 caagaatgaa cttactgaaa tggatctcag cggtaaggat ctgcgtaacc tactggagca
     3001 cggggcatcg cttaccaatg gcatcctgca aatgtcaaaa ggcgcagaaa tgcgctatac
     3061 cctgcagaaa ccagtaggtc agcgcattgt atcttttaaa attaatggtg aagagatcgt
     3121 tgatacgaat atttaccatg ttgcgactac gactttcctt gcacttgggg gcgatggttt
     3181 tctggcattc aaagaaggga aaaatgtcca ggtccgcgcc ggaaataaca tgtccaatgt
     3241 cgtaatcgat tattttaaga aaggccacaa gattactcct gcacaggtaa atgaaatgcg
     3301 ggtagaagta agcaaataac aaaaacttat aataccattc attcactgga tgacatt
//
LOCUS       DAZFJL010000231         3329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFJL010000231 DAZFJL010000000
VERSION     DAZFJL010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.233"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            225..728
                     /locus_tag="ACHJ8C_004567"
     CDS             225..728
                     /locus_tag="ACHJ8C_004567"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5554279.1"
                     /translation="MNVVASPALRLRKLTVADNPAIAHVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLRKL"
     gene            complement(905..1321)
                     /gene="rraB"
                     /locus_tag="ACHJ8C_004568"
     CDS             complement(905..1321)
                     /gene="rraB"
                     /locus_tag="ACHJ8C_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO5554280.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGIRH"
     gene            1483..2487
                     /gene="argF"
                     /locus_tag="ACHJ8C_004569"
     CDS             1483..2487
                     /gene="argF"
                     /locus_tag="ACHJ8C_004569"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO5554281.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEARLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVAECSALAQKHGGKITLTED
                     IASGVKGADFIYTDVWVSMGEPKEKWAERIALLRDYQVNSKMMALTGNSQVKFLHCLP
                     AFHDEQTTLGKKMAAEFGLYGGMEVTDEVFESPASIVFDQAENRMHTIKAVMVATLAK
                     "
     gene            complement(2532..2984)
                     /locus_tag="ACHJ8C_004570"
     CDS             complement(2532..2984)
                     /locus_tag="ACHJ8C_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO5554282.1"
                     /translation="MIVGNIHHLQSWLPEELREAIEYIKSHVSDETAKGKHAIDGDRL
                     FYLISEDTTEPGELRRAEYHARYLDIQIVLKGQEGMTFSTQPASVPETDWLADKDIAF
                     IGQGIDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKLLKA"
BASE COUNT          847 a          805 c          760 g          917 t
ORIGIN      
        1 gaattatctt tcccgatgtt tacatcattc atgaactata tccctaaaat aaattccatt
       61 cataccgata ataattccag tcaattgata ctggttcgcc tagaatacgc tttatctcag
      121 agagatatgc gtaatatttt ctgatttatc ttcttcttgc accacctaag cgagggtggt
      181 attaaagtga gctataaatc atacatttga aattgagcta tgctatgaat gttgtcgctt
      241 ctcccgcact ccgtttgcgc aaacttaccg tcgccgataa tcctgctatc gcccacgtca
      301 ttcgtcaggt atccgccgaa tacggtctta ccgctgataa aggctacacc gtcgccgatc
      361 cgaaccttga cgagctgtat caagtgtata gtcagcctgg ccacgcatat tgggtggttg
      421 agtacgaggg tgaagtggtc ggcggcggtg ggattgcgcc attaaccggg agtgagtcag
      481 acatttgcga actgcaaaag atgtattttc ttccggctat ccgcggcaaa gggctggcaa
      541 aaaaactggc cttaatggca atggagcagg cgcgagaaat gggtttcaaa cgctgctatc
      601 tggaaacgac cgctttttta aaggaagcca ttgcgcttta tgagcatttg ggctttgagc
      661 atatcgacta tgcgcttggc tgcacgggtc atgtcgattg tgaagtacgg atgctacgca
      721 aactttgaaa aatgcccgcg agctgagact cgcgggcatt cgaacattaa ccacgtgctt
      781 tttgagtatg ctggatgtga cgtaaatgcc ttatccgggc tacaaattca ccaggttgca
      841 ggaactacct gggcctgata tgcgtagcgc atcaggtaac gcagcctttg tcgtcaacgc
      901 ttaattagtg acgaataccg tcatcgtctt catcgacaaa atcttcatcg tcgccgtctt
      961 cgccgttagg atcttcaaag taagtgcccc aaccatcgta ctcaacgtca aatttctccg
     1021 ccagcgtcat cagttgttca acctgggcat cgatcaggtc ggcattcaac gcacactcgc
     1081 tgaggatgtc gcagcaaatc acgatgtcac catcttcaac ttccagctct tctggatcgg
     1141 tcacttcgta accgagttta aacgcttcaa ctgctgcttt ttccagggtt tccagatcgt
     1201 ctgcggaaag atggtgttcg atggtgtaca gcgcgtccgg atcgctgcca tcttccagta
     1261 attcttcaat aatcaaacgt gtttcttcac gctgttcttc cagttgttcc gggtttgcca
     1321 tggctcattc ctcttaatgt cctgccgata ctcttattgt cacacaccac tgacattgcc
     1381 tccacctttg tgacaaagat ttatgcttta gacttgcaaa tgaataatca tccatataaa
     1441 ttgaatttta attcattaag gcgttagcca caggagggat ctatgtccgg gttttatcat
     1501 aagcatttcc tgaaattact cgatttcacg ccagctgaac tcaacagctt gttgcagtta
     1561 gccgcgaagc tgaaagccga taagaaaagc ggtaaagaag aagccagact cactggcaaa
     1621 aacatcgcgc tcatcttcga aaaagactcg acccgtaccc gatgctcttt cgaagttgcc
     1681 gcatatgacc agggtgctcg cgttacttat ctcggcccaa gcggcagcca gattggtcac
     1741 aaagagtcga ttaaagacac tgcccgcgtg cttggtcgca tgtatgacgg tattcagtat
     1801 cgcggctatg gtcaggagat tgtcgaaaca ctggcggaat acgctggcgt gccagtatgg
     1861 aacggcctga ccaatgagtt ccaccccact cagctgctgg cggatctcct caccatgcag
     1921 gagcatttgc ccggcaaagc attcaacgaa atgacgctgg tctatgcagg tgacgcgcgt
     1981 aacaacatgg gcaattcgat gctcgaagct gcggcgctta ccggtctgga tttgcgtctg
     2041 gtcgcgccac aggcatgctg gccggaagct gcgctggtcg ccgaatgcag tgctctggca
     2101 caaaaacacg ggggtaaaat caccttgacg gaagatatcg cctctggggt taaaggtgca
     2161 gactttatct ataccgatgt ctgggtatcg atgggtgagc ccaaagagaa atgggcggag
     2221 cgtattgccc tgctgcgtga ctatcaggtg aatagcaaaa tgatggccct gaccggtaat
     2281 tcgcaggtta agttcctcca ctgtctgcct gcatttcatg atgagcaaac cacgctgggt
     2341 aaaaaaatgg ccgcagaatt tggtctgtat ggtggaatgg aagtcaccga tgaagtcttt
     2401 gaatctccgg caagtattgt ctttgaccag gcagaaaacc ggatgcacac cataaaagct
     2461 gttatggtcg ctacattagc aaaataacgt tttgccgggc ggcggcacag gtcgcccccg
     2521 gcagtgatca cttacgcttt taataactta actactgctt tccgtacctg agcgggggct
     2581 cccaccgcac aaagcggttt gtgaacttcg ccaggataga aaacgacaaa atccccttca
     2641 ttaagaatca ctgttttttc atcgatcccc tgcccgataa aagcaatatc tttatctgcc
     2701 agccagtcag tctctggtac gcttgctggt tgcgtgctaa atgtcatccc ttcctgccct
     2761 tttaatacaa tctggatatc gagataacga gcatgatact cagcacgacg cagttcacct
     2821 ggttcagtcg tatcctcaga aatcagataa aacaggcggt cgccgtcaat agcgtgctta
     2881 ccttttgctg tctcatcgct gacatgtgat ttgatgtact caattgcttc gcgaagttct
     2941 tccggcagcc aggattgtaa gtgatggatg ttgccaacaa tcataatatc ttcctttatt
     3001 aaaacaatgt ttcacttctt tccttatacg aaaaaaagcc acctctcacc atagaaaaat
     3061 catcaggttt tgcccttgcg catgtttatt gctggagatg ttgtactgat gttaagcagg
     3121 agtaattttg tatgcagttt tgctgcataa atcttataac ttagctatgt attttggtcc
     3181 taatatcttg ttattgcata tgttaaaaga aaactctcag tcagatcaca gttcattttc
     3241 ttagctttta atgcccgtgc tgtcatagtt atcccgtgaa aatagtgctg tgaaaaataa
     3301 tcaccttatc ctttcgcata gatatgcaa
//
LOCUS       DAZFJL010000232         3298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFJL010000232 DAZFJL010000000
VERSION     DAZFJL010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.234"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            161..1681
                     /locus_tag="ACHJ8C_004571"
     CDS             161..1681
                     /locus_tag="ACHJ8C_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO5554283.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALEHPKPTNAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(1706..2044)
                     /gene="maoP"
                     /locus_tag="ACHJ8C_004572"
     CDS             complement(1706..2044)
                     /gene="maoP"
                     /locus_tag="ACHJ8C_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO5554284.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2163..3002
                     /gene="hdfR"
                     /locus_tag="ACHJ8C_004573"
     CDS             2163..3002
                     /gene="hdfR"
                     /locus_tag="ACHJ8C_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO5554285.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDVHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFCSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(3098..3173)
                     /locus_tag="ACHJ8C_004574"
     tRNA            complement(3098..3173)
                     /locus_tag="ACHJ8C_004574"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3138..3140),aa:Trp,seq:cca)
     gene            complement(3182..3258)
                     /locus_tag="ACHJ8C_004575"
     tRNA            complement(3182..3258)
                     /locus_tag="ACHJ8C_004575"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3222..3224),aa:Asp,seq:gtc)
BASE COUNT          888 a          815 c          805 g          790 t
ORIGIN      
        1 gttcatcctt tgcacgctgc atttttgcga gcatcattcc accgccagaa caactcaact
       61 tcacttgcac aaataaacag gaatcagcca ctaaaattca cgaattagcg ggctacagca
      121 gcgatatctt atgcacataa tggtgactca aggagggatt atgtcactgt caattgttca
      181 tacccgcgca gccctgggag taaatgcgcc cccaatcact gttgaggtac atatcagtaa
      241 aggtctacca ggcttaacga tggtgggctt accagaaaca acggtaaaag aagctcgcga
      301 tcgcgtgcgc agcgccatta tcaatagcgg atatgaatat ccggcgaaaa aaatcaccat
      361 caaccttgct ccagccgatc tgccaaaaga agggggacga tatgatttgc ctatcgctat
      421 tgcgttactg gcggcctcag aacagcttac agccaataag ttagatgaat atgaattagt
      481 cggagaactg gcgcttacag gcgctctgcg tggcgttccc ggcgcaatct ccagtgcaac
      541 tgaagctatt aagtcgggca gaaaaattat cgtcgcgaaa gataacgaag atgaagtggg
      601 gctaattaac ggtgaaggat gcctgatagc cgatcatctg caggctgtct gcgcgtttct
      661 ggaaggtaag cacgctctcg aacacccgaa accaactaat gcagtatccc gggcgctaca
      721 acatgatctc agtgatgttg tcggtcagga gcaaggaaag cgaggactgg aaattaccgc
      781 cgctggcggg cacaaccttt tactgattgg accgccggga acaggtaaaa caatgctcgc
      841 cagccgtatt aatggccttt tgccagattt aagcaatgaa gaggcactgg agagcgctgc
      901 gatattaagt ctggtaaatg ctgaatcagt acaaaaacaa tggcggcagc gcccgttccg
      961 ctcacctcat cacagtgcgt cgttaactgc gatggtgggc ggtggtgcaa ttccagggcc
     1021 aggtgaaatt tcgctggcgc ataacggcgt gctttttctt gatgagctac ctgaatttga
     1081 acggcgtaca ctggatgcct tgcgagagcc gattgaatcc gggcagatcc atctttcacg
     1141 cacacgagca aaaataacct atccagcccg tttccagctt gtcgcggcga tgaatcccag
     1201 ccctaccgga cattatcagg gaaaccataa ccgctgcaca ccagaacaga cattacgtta
     1261 tctcaaccgg ctctcggggc cctttctcga ccgcttcgat ctctcactgg agatcccatt
     1321 accgcccccc ggcattttga gtaaaacggt agtgccggga gaaaacagca ccaccgttaa
     1381 acaacgtgta atggccgcca gagagcgcca atttaagcgg cagaataaac tgaacgcctg
     1441 gctggataat ccggaaatac gccaattctg caagcttgaa agcgaagatg cgcagtggct
     1501 ggaagaaacg ctgatccatc tggggttatc gattcgtgcc tggcagcggt tattgaaagt
     1561 tgcacgaacc attgctgata ttgatcagtc tgacattatc acacgtcagc atttgcagga
     1621 ggcagttagc tatcgtgcga ttgaccgttt gctcatccat ctgcaaaaac tactgacata
     1681 aaaaaagggc atttcgccct ttttattaat cgtcagaatc ggtgtagtct tcagcacctt
     1741 caacctgcgg tttaccgccg gaaagggtgt gaaaacgttt tggacgcttg atacgcgtca
     1801 tatacttgga ccacacgcgt tctgcttctg tcactggttc acgttcgcca cggcatactg
     1861 ctacgaagag tttctcttcc tcggtaaccg gctcgcgttt gccaagatcc aactcattga
     1921 aggcataacc atgacgctca agcagttgtg cctctttgat ggtgaagtca ccatgacgag
     1981 agaatccacg tggataatgt ttattgtcga aatatcgatt agtcgtcgta aagctttccg
     2041 ccatcctgca cgctcctaat tctttgaccg agctagttat ggcgcggagt attagttacg
     2101 cttgacagag tgtaaaacaa aacatttaaa tcataacgac aaataatttt gcggagagca
     2161 ctgtggatac ggaattgtta aaaactttcc tggaagttag ccgaacgcgt cactttggtc
     2221 gagcggctga atcgctctat ctgacccaat cagccgtgag ctttcgaatc agacaactgg
     2281 aaaatcaact gggtgtgaat cttttcaccc gccacagaaa caatatccgt ttaaccgctg
     2341 ccggtgaaaa actactgcct tatgcagaaa cgctcatgag cacctggcag gccgcccgca
     2401 aggaggtggc gcatacctca cgacataatg agttttctat cggtgccagc gcctcgctgt
     2461 gggagtgtat gcttaatcag tggctgggac gcttgtatca aaatcaggat gtccatacag
     2521 gcttacagtt cgaagcgcga attgcccaac ggcagtctct ggtaaaacag ttgcatgaac
     2581 gccagcttga tcttcttatt acaactgaag cccccaaaat ggacgaattt tgtagtcagt
     2641 tgctggggta tttcacttta gcgctttata ccagtgcccc ttctaaacta aagggagatc
     2701 ttaactatct gcgactagag tgggggccag attttcaaca gcatgaggca ggtttgatcg
     2761 gtgctgacga agttcccatt ctgacaacca gttctgctga actggcacag caacagattg
     2821 cgatgcttaa tggttgcacc tggctacccg tcagttgggc acgtaaaaaa ggcggcctgc
     2881 ataccgttgt cgatagcaca acactttcac ggccgcttta tgccatatgg ctacaaaata
     2941 gcgataaaaa tgcgttgatt cgcgatctat tgaaaattaa cgtgctggat gaagtgtatt
     3001 aatctgaatg gctggcaagg atgccggtag aaggatttac ttcggagagg gttatttcag
     3061 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg caggggcgga gagactcgaa
     3121 ctcccaacac ccggttttgg agaccggtgc tctaccaatt gaactacgcc cctaattagg
     3181 gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag gtattctaac
     3241 cgactgaact accgctccac cgaattcttt tacaaccacc ggtttataac cggcttac
//
LOCUS       DAZFJL010000233         3221 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFJL010000233 DAZFJL010000000
VERSION     DAZFJL010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.235"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(95..622)
                     /gene="mobB"
                     /locus_tag="ACHJ8C_004576"
     CDS             complement(95..622)
                     /gene="mobB"
                     /locus_tag="ACHJ8C_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO5554286.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQDG"
     gene            complement(604..1188)
                     /gene="mobA"
                     /locus_tag="ACHJ8C_004577"
     CDS             complement(604..1188)
                     /gene="mobA"
                     /locus_tag="ACHJ8C_004577"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO5554287.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQVSGLKVIEDSLADYPGPLAGMLSVMQQESGEWFLFCPCDT
                     PYIPHDLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDRKEAFINVNTPEELARWQEKR"
     gene            1258..1527
                     /locus_tag="ACHJ8C_004578"
     CDS             1258..1527
                     /locus_tag="ACHJ8C_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO5554288.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1604..2590
                     /gene="srkA"
                     /locus_tag="ACHJ8C_004579"
     CDS             1604..2590
                     /gene="srkA"
                     /locus_tag="ACHJ8C_004579"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO5554289.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTVTFIEQAKVLQEPPLQLTPMY"
     gene            2607..>3221
                     /gene="dsbA"
                     /locus_tag="ACHJ8C_004580"
     CDS             2607..>3221
                     /gene="dsbA"
                     /locus_tag="ACHJ8C_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO5554290.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKELTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLS"
BASE COUNT          805 a          779 c          795 g          842 t
ORIGIN      
        1 gcgaagagcc cagtcggaag actgggcttt tttgcgttgg tgcggaataa atgccggatg
       61 cgacgctggc gcgtttgctc cagcctactc ttgcttatcc gtcctgcttt tgcatccact
      121 ccaccacaaa atcagccagc ccctcaacat cattaatatc cagtaacgcg acatcaagat
      181 taagcggcac atcactggct acagcaataa catgcctgtc tatcactaat tcttccggtc
      241 gatgtccggc tccatcgcga aacagcacaa tctttgcgat ctcttcatgc ttaaaccctt
      301 cgaccagaat caaatccagc tttgaggtat ccattcgact tgcgagaaaa tgtagatcca
      361 gctcttcttc gtctggtgtt tccgtcatca aggcccatcg ctgttggctg gcaacgatgg
      421 tttgcgccgc gccagccttg cgcagctcat agctatcttt gcccggttta tcaacatcca
      481 tatcatggtg cgtatgctta atcagccccg gacggatccc tctggcgcat aatgccggga
      541 tcaatttttt cagcagcgtt gtttttccgg tgccactcca tgcagcaaag gcgagtaacg
      601 gtatcatcgt ttttcctgcc atcgggcaag ctcctctggc gtattcacgt ttataaatgc
      661 ctctttacga tcgctgaaat caaccgcatg accgccagcc agacgcataa atgccataac
      721 ccggcgttct cctgcttgca gatattccag taataaaggc tcaattgcgc ggtttaccag
      781 agcaatagtc gggtgatcgc gttcaccgtc gtggacccac acgacaggcg catctttgcg
      841 ctgatgatta agccgggagg ctaaatcatg gggaatgtaa ggcgtatcgc acgggcaaaa
      901 caaaaaccac tcacccgatt cctgctgcat tactgaaagc attcctgcca gagggccagg
      961 gtaatccgcc agtgaatctt caatcacttt cagaccgctt acctgataga tttcctgatg
     1021 acgattagca ttaaccacga cgtgagaaag ctgcgtcata agcgcgtcag cgacatgttg
     1081 ccataatggt ttgccgttta attcaagcaa tcctttatct acgccgccca ttcgtctggc
     1141 tttaccgccc gccagcacaa cgcctgttat cgtcgtcatc agattcaccg atatcgcctc
     1201 ttttattgtg ggattgaccc tgctaacgtg tctgtctcaa gagtaaggag cattactatg
     1261 aaatgtaaac gtctgaatga agttattgaa ctcctccagc cagcctggca aaaagagccg
     1321 gaccttaacc tgctgcaatt tttgcagaaa ctggcgaaag agtcaggttt tgacggcgaa
     1381 ctggcggatt tgacggatga cattctgatc tatcacctga aaatgcgtga ttccgcgaaa
     1441 gatgcggtga tcccgggttt gcagaaagat tatgaagaag atttcaaaac ggcgctatta
     1501 cgcgcacgtg gcgtaattaa agagtaaaag cttgtaagcg gcgcaaccaa aatcatcgtg
     1561 aaatgatatc cttcatcatt cgtaatgttt tccggatgat gggatgaaca acagcgcttt
     1621 tactttccag acactacacc cggataccat catggacgct ctgtttgagc atgggattcg
     1681 ggtggattcc ggtcttaccc cgcttaacag ctatgaaaac cgtgtctatc aatttcagga
     1741 cgaagatcgt cgacgttttg tcgtcaaatt ttatcgccct gaacgttgga cagccgatca
     1801 aatcctcgaa gaacatcaat ttgcgttgca gctggttaac gatgaagttc cggtcgcagc
     1861 acctgtggcc tttaatggtc agactttatt gaaccatcag ggattttatt tcgctgtttt
     1921 tccaagcgtc ggtggtcgcc agttcgaagc tgataatatc gatcagatgg aagcggttgg
     1981 gcgttattta gggcgtatgc accagacggg gcgcaaacag ctttttatcc atcgcccgac
     2041 catcggtttg aatgaatatc tcattgagcc acgcaagctg tttgaggacg ctacactgat
     2101 accttccggg ttgaaagcgg cattcctgaa agcgacagat gagctgattg ccgccgttac
     2161 agcacactgg cgggaagatt tcaccgttct gcggctacat ggagactgcc acgccgggaa
     2221 tattctctgg cgcgatggtc caatgtttgt tgatctggat gatgcacgta atggtccagc
     2281 cgttcaggat ttgtggatgt tgctcaatgg tgataaagcc gagcagcgga tgcaactgga
     2341 aactattatt gaggcttatg aagaatttag cgagttcgac accgctgaaa tcggactgat
     2401 tgaaccttta cgcgccatgc gtttggttta ttatcttgcc tggctaatgc ggcgttgggc
     2461 tgatcccgcg ttcccgaaaa atttcccgtg gttaaccggg gaagattact ggctacgaca
     2521 gacggtgact tttatagaac aggcaaaagt tctacaagaa ccccctttgc aattaacacc
     2581 tatgtattaa tcggagagag tagatcatga aaaagatttg gctggcgctg gctggtttag
     2641 ttttagcgtt tagcgcatcg gcggcgcagt atgaagatgg taaacagtac actaccctgg
     2701 aaaaaccagt tgctggcgcg ccgcaagtgc tggagttttt ctctttcttc tgcccgcact
     2761 gctatcagtt tgaagaagtt ctgcatattt ctgataacgt gaagaaaaaa ctgccggaag
     2821 gcgtgaagat gactaaatac cacgtcaact tcatgggggg tgacctgggc aaagagctga
     2881 ctcaggcatg ggctgtggcg atggcgctgg gcgtggaaga caaagtcaca gttccgctgt
     2941 ttgaaggcgt acaaaaaacc cagaccattc gttcagcatc tgatatccgc gatgtattta
     3001 tcaacgcagg tattaaaggt gaagagtacg acgcggcgtg gaacagcttc gtggtgaaat
     3061 ctctggtcgc tcagcaggaa aaagctgcag ctgacgtgca attgcgtggt gttccggcga
     3121 tgtttgttaa cggtaaatat cagctgaatc cgcagggtat ggataccagc aatatggatg
     3181 tttttgttca gcagtatgct gatactgtga aatatctgtc c
//
LOCUS       DAZFJL010000234         3215 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZFJL010000234 DAZFJL010000000
VERSION     DAZFJL010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.236"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..1531)
                     /locus_tag="ACHJ8C_004581"
     CDS             complement(<1..1531)
                     /locus_tag="ACHJ8C_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5554291.1"
                     /translation="MNLKLKRCEYWMAAQKQMKRVVPLLLVIMPACSIAGMRFNPAFL
                     SGDTEAVADLSRFEKGMTYLPGSYEVEVWVNDSPLLSRTVTFKADDANQLIPCLSLAD
                     LLSLGINKNALPEQALASSENSCLDLRIWFPDVHYMPELDAQRLKLTFPQAIIKRDAR
                     GYIPPEQWDNGITAFLLNYDFSGNNDRGDYSSNNYYLNLRAGINIGAWRFRDYSTWSR
                     GSNSAGKLEHISSTLQRVIIPFRSELTLGDTWSSSDVFDSVSIRGIKLESDENMLPDS
                     QSGFAPTVRGIAKSRAQVTIKQNGYVIYQTYMPPGPFEISDLNPTSSAGDLEVTIKES
                     DNSETVYTVPYAAVPILQREGHSKYSTTVGQYRSNSYNQKSPYIFQGELIWGLPWDIT
                     AYGGAQFSEDYRALALGLGLNLGVFGATSFDVTQANSSLVDGSKHQGQSYRFLYSKSL
                     VQTGTAFHIIGYRYSTQGFYTLSDTTYQQMSGTVVDPKMLDDKDYVYNWNDFYNLRYS
                     KR"
     gene            complement(1568..2320)
                     /locus_tag="ACHJ8C_004582"
     CDS             complement(1568..2320)
                     /locus_tag="ACHJ8C_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5554292.1"
                     /translation="MKCIIGNKKWVYGFRDELKVSQNIIRSGILFLLSILFINNASAG
                     GFGLGVTRLIYPAESKQITLSVQNSGDNTSYLIQSWVDDSKTEKKSQDFVITPPLFML
                     PTRKEASLRIMFLGKTNLPTDRETLYWMNVKAIPPTDEKNTQKNTLQLALQNKIKLFY
                     RPENLPVQPGKARNMLRFKYEGKQLKVINPSPYYLTLTGIKVQGSKLPNMFVPPKSDI
                     TVSSPVTLAGEITYQTINDFGATTERQKGIMQ"
     gene            complement(2421..3125)
                     /locus_tag="ACHJ8C_004583"
     CDS             complement(2421..3125)
                     /locus_tag="ACHJ8C_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5554293.1"
                     /translation="MKFNLSNLSAVLLASGMLMSTAVTAAPGDATQFGGADTDWSTVD
                     YPRLTDMDDNVDSMGGKIRFTGRVVKATCKVATDSKQIEVVLPVVPSNLFTGIDVEAQ
                     GASNQTDFNINLTECSNTDDQKIEFRFTGTADSANKTLANEVEGSTDADNSGNAGATG
                     VGIRIYSKGTTNNGLINLNTTAAEGSASTAAYTIPGNATTHDFSAAFTAGYAQNGSTV
                     APGVVKSTASFVVLYE"
BASE COUNT          914 a          730 c          623 g          948 t
ORIGIN      
        1 cacgtttgct ataacgcaag ttataaaaat cattccagtt ataaacgtaa tctttatcat
       61 ctaacatttt tggatcaaca acagtccctg acatttgttg gtatgtcgta tcacttaaag
      121 tgtaaaagcc ctgggttgaa taacgatagc caataatatg gaatgctgtt cctgtctgaa
      181 ctaacgattt ggaataaaga aaacgataag attgcccttg atgtttgctc ccatccacaa
      241 gcgaactgtt agcctgagta acatcaaacg atgttgcacc aaatactccc agattcaggc
      301 caaggccgag cgccaacgcc cggtaatcct cagagaattg tgccccacca taagccgtaa
      361 tatcccaggg taaaccccaa attaattccc cctgaaatat ataaggactt ttctggttat
      421 agctattgct tcgatattgg ccaaccgtag tagaatattt tgaatgacct tctcgttgca
      481 ggatggggac agcggcataa ggtacggtat agacagtttc tgaattatca gactctttga
      541 tggtaacttc cagatctccc gcagatgatg tcgggttaag atcgctaatc tcaaacggtc
      601 ccggcggcat ataggtttga taaatgacat aaccattctg tttgattgtt acctgagcgc
      661 gacttttcgc aattccgcgc accgtgggag cgaaaccact ttgactatcg ggcaacatat
      721 tttcgtcaga ttccagtttt atgccacgaa tactaacact gtcgaaaaca tctgatgatg
      781 accatgtatc tcctagcgtt aattcacttc tgaaaggaat aataacgcgc tgcaacgtac
      841 tactgatatg ctccaattta cctgctgaat tactcccacg actccaggtt gaataatcgc
      901 gaaaacgcca tgcaccaata ttgatcccag cgcgaagatt taaataatag ttatttgaag
      961 agtaatcacc acgatcgtta ttaccagaaa agtcataatt cagcaaaaaa gctgtaatac
     1021 cgttatccca ctgttctggt ggaatatatc cgcgggcgtc acgttttatt atcgcctgcg
     1081 gaaaggtcag tttaagtctc tgtgcatcca gctccggcat gtaatgcaca tcaggaaacc
     1141 agatacgcaa atcaaggcaa ctattttcag atgaagccaa agcctgctct ggcagcgcat
     1201 ttttgttaat tccaaggctt aataagtcag caagtgaaag gcagggaatc agttgattcg
     1261 catcgtctgc tttaaaagtt acagtacgag agagtaaagg ggaatcattg acccaaactt
     1321 cgacttcata gctaccagga agataagtca tccctttctc gaagcgggat aagtcagcaa
     1381 cagcttcagt atcacccgac agaaaagcag ggttaaagcg cattcccgcg attgaacatg
     1441 caggcataat aaccagaaga agcggcacaa cccgtttcat ctgcttttgt gccgccatcc
     1501 aatattcgca tcttttgagc tttaggttca tatccctgac ctgtgttgca tctgaactat
     1561 tccgttgtta ctgcattatt cctttctgcc gctccgtcgt agcgccaaag tcattaattg
     1621 tttgataagt aatttcacca gccaacgtca ctggcgacga gaccgtaata tcacttttcg
     1681 gtggtacaaa catattgggc aatttactcc cttgtacttt aattcctgtc agtgtcaggt
     1741 aataaggcga aggattgata acttttagtt gtttgccctc atacttaaac cgcaacatat
     1801 ttcgtgcttt tcctggttgc acaggtagat tttccgggcg ataaaatagc ttgattttgt
     1861 tttgtaatgc caactgtaag gtattcttct gtgtattttt ctcatctgtt ggcggaattg
     1921 ctttaacatt catccaatac aaagtttcac gatcggtcgg caaattagtc ttaccaagaa
     1981 acatgatacg taatgaagct tctttacgcg ttggcaacat aaagagggga ggagtgataa
     2041 caaaatcctg gctctttttt tccgttttac tgtcatcaac ccatgattga attaaataac
     2101 tggtgttgtc accgctattt tgcacactga gtgttatttg cttactttct gccggataaa
     2161 tcaatcgcgt tacgccaagg ccaaacccgc ccgcggacgc attattaata aataatatcg
     2221 atagtaaaaa aagaatccct gagcgtataa tattttgtga tacttttaat tcatccctga
     2281 atccataaac ccatttttta tttccaataa tacatttcat acagcatcct tgttgatgaa
     2341 ataacacctg ttattcatac tctattaaat aatttcaatc tcaggttaca actttttgta
     2401 acctgaaatc gtatttatat ttactcgtac agcaccacaa aacttgctgt tgacttaact
     2461 acaccaggtg caacagtgct accgttttga gcataacctg cagtaaaggc cgcgctgaaa
     2521 tcatgggtcg tagcatttcc tggaattgta taagcggcgg tggaggcgct accctctgcc
     2581 gcagtagtat tcaggttaat cagaccatta ttcgtcgtac ccttggagta aattcgaatc
     2641 cctacaccag tcgcccccgc attgccgctg ttgtcagcat ccgttgatcc ttctacttcg
     2701 ttagcgagcg ttttattagc gctatctgca gtaccggtaa aacggaactc aattttctga
     2761 tcatctgtat tgctacattc ggtcagatta atattgaaat cggtctggtt gctcgccccc
     2821 tgtgcttcta cgtcgatacc agtgaaaagg ttggaaggca caaccggcag gacaacttca
     2881 atctgttttg aatcggttgc gaccttacag gtagctttca ctacacggcc agtaaagcgg
     2941 attttccccc ccattgaatc aacgttgtca tccatatcag tgagcctggg ataatcaacg
     3001 gtgctccagt cagtatccgc cccaccaaat tgtgttgcat cgccgggtgc tgcggttacc
     3061 gcagtagaca tcagcatacc tgatgccagt aatactgcgg ataaattaga taaattgaat
     3121 ttcatttata cctttccttt tgttaaaaaa tccaagcaac attggtgttt ttacattctg
     3181 cgttaataag ttatatctca caaaaaaaaa taagc
//
LOCUS       DAZFJL010000235         3191 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZFJL010000235 DAZFJL010000000
VERSION     DAZFJL010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.237"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..2649
                     /gene="eae"
                     /locus_tag="ACHJ8C_004584"
     CDS             <1..2649
                     /gene="eae"
                     /locus_tag="ACHJ8C_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin"
                     /protein_id="HFO5554294.1"
                     /translation="SYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEM
                     MKAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTD
                     DKALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNL
                     QSGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPENMLGYN
                     VFIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIR
                     FNGYLPSYPALGARLMYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDY
                     RHGTGNENDLLYSMQFRYQFDKPWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYK
                     KQDILSLNIPHDINGTERSTQKIQLIVKSKYGLDRIVWDDSSLRSQGGQIQHSGSQSA
                     QDYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTA
                     DKTSAKADGNEAITYTATVKKNGVAQANVPVSFNIVSGTAVLSANSANTNGSGKATVT
                     LKSDKPGQVVVSAKTAEMTSALNANAVIFVDQSKASITEIKADKTTAVANGKDAITYT
                     VKVMKNGQPVSGEEVTFTKTLGTLSKPTEKTDANGYAKVTLTSATQGKSLVSARVSDV
                     AVDVKAPEVEFFTPLTVDDGNIEIVGTGVKGKLPTVWLQYGQVKLKASGGNGKYTWRS
                     ANTAIASVDASSGQVTLKDKGTTTITVVSSDNQTATYTIATPNSLIVPNMSKRVTYND
                     AVNTCKILGGRLPSSQDELKNVFNTWGAANKYEYYKSLLTISSWVQQTTVDMNSGVAS
                     TYDLVRQNPLNGVETNTTNAYATCVK"
     gene            complement(2866..>3191)
                     /gene="escD"
                     /locus_tag="ACHJ8C_004585"
     CDS             complement(2866..>3191)
                     /gene="escD"
                     /locus_tag="ACHJ8C_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFO5554295.1"
                     /translation="NDVIIVAGEVSQQNESKILAIINAMNKNSNVKILFQNIQPYISA
                     DIFPGKILRISGTMKNPTIALDNGTSLGIGSILKGGYVIDAIDPKDGINISRPDEYIH
                     IPLSY"
BASE COUNT          931 a          635 c          724 g          901 t
ORIGIN      
        1 agctatcaga atcgcctttt ttatacgttg aaaacaggtg aaactgttgc cgatctttct
       61 aaatcacaag atattaattt atcgacgatt tggtcgttga ataagcattt atacagttct
      121 gaaagcgaaa tgatgaaggc cgcgcctggt cagcagatca ttttgccact caaaaaactt
      181 ccctttgaat acagtgcact accactttta ggttcggcac ctcttgttgc tgcaggtggt
      241 gttgctggtc acacgaataa actgactaaa atgtccccgg acgtgaccaa aagcaacatg
      301 accgatgaca aggcattaaa ttatgcggca caacaggcgg cgagtctcgg tagccagctt
      361 cagtcgcgat ctctgaacgg cgattacgcg aaagataccg ctcttggtat cgctggtaac
      421 caggcttcgt cacagttgca ggcctggtta caacattatg gaacggcaga ggttaatctg
      481 cagagtggta ataactttga cggtagttca ctggacttct tattaccgtt ctatgattcc
      541 gaaaaaatgc tggcatttgg tcaggtcgga gcgcgttaca ttgactcccg ctttacggca
      601 aatttaggtg cgggtcagcg ttttttcctt cctgaaaata tgttgggcta taacgtcttc
      661 attgatcagg atttttctgg tgataatacc cgtttaggta ttggtggcga atactggcga
      721 gactatttca aaagtagtgt taacggctat ttccgcatga gcggctggca tgagtcatac
      781 aataagaaag actatgatga gcgcccagca aatggcttcg atatccgttt taatggctat
      841 ctgccatcat acccggcatt aggtgccagg ctgatgtatg agcagtatta tggtgataat
      901 gttgctttgt ttaattctga taagctgcag tcgaatcctg gtgcggcgac cgttggtgta
      961 aactatactc cgattcctct ggtgacgatg gggatcgatt accgtcatgg tacgggtaat
     1021 gaaaatgatc tcctttactc aatgcagttc cgttatcagt ttgataaacc gtggtctcag
     1081 caaattgagc cacagtatgt taacgagtta agaacattat caggcagccg ttacgatctg
     1141 gttcagcgta ataacaatat tattctggag tacaaaaagc aggatattct ttctctgaat
     1201 attccgcatg atattaatgg tactgaacgc agtacgcaga agattcaatt gatcgttaag
     1261 agcaaatacg gtctggatcg tatcgtctgg gatgatagtt cattacgtag ccagggcggt
     1321 cagattcagc atagcggaag ccaaagcgca caagattacc aggctatttt gcctgcttat
     1381 gtgcaaggtg gcagcaatat ttataaagtg acggctcgcg cctatgaccg taatggcaat
     1441 agctctaaca atgtacagct tactattacc gttctgtcga atggtcaagt tgtcgaccag
     1501 gttggggtaa cggactttac ggctgataag acatcggcta aagcggacgg caacgaagcg
     1561 attacttata ccgcgacggt caaaaagaat ggggtagctc aggctaatgt ccctgtttca
     1621 tttaacattg tttcaggaac tgcagtttta agtgccaata gtgccaatac caatggtagc
     1681 ggtaaggcga ctgtaacgct gaaatcggat aaaccaggcc aggtcgtcgt gtctgctaaa
     1741 acagcggaga tgacttcagc gcttaatgcc aatgcggtca tatttgttga tcaaagtaag
     1801 gctagtatta ccgagattaa ggctgataaa acaactgcag tagcaaatgg taaggatgct
     1861 attacataca ctgttaaagt gatgaaaaat ggtcagccgg tatccggcga agaagtgacc
     1921 tttacgaaga ccttaggtac attaagtaag cccactgaaa aaacggatgc gaatggctat
     1981 gctaaagtaa cattaacatc ggcgactcaa gggaaatccc tcgttagtgc ccgtgttagc
     2041 gatgtcgccg ttgacgtcaa agcccctgaa gttgaatttt ttacaccact tacagttgat
     2101 gacggtaata ttgaaattgt tggaaccgga gttaaaggga agttacctac tgtatggttg
     2161 caatatggac aagttaagct gaaagctagc ggaggtaacg gaaaatatac atggcgctca
     2221 gcaaatacag cgattgcttc ggtggatgcc tcttctggtc aggtcacctt aaaagataag
     2281 ggaactacaa ctattaccgt tgtctcaagt gataatcaaa ccgcaactta tactattgca
     2341 acacctaata gcctgattgt tcctaatatg agcaagcgtg tgacttataa tgatgctgtg
     2401 aatacatgta agattttggg aggaagatta ccctcttctc aggatgaact gaaaaatgtc
     2461 tttaacacat ggggagcagc aaataaatat gaatattaca agtctcttct gactataagc
     2521 tcttgggtac aacaaacaac agtggatatg aacagtggag ttgcaagtac atacgatcta
     2581 gttagacaaa accctcttaa tggcgttgaa actaatacta ctaatgctta tgccacttgt
     2641 gtaaaataaa cttaataaat atctaatcat tgtccggcta aaatgtgtgt tttagtcgga
     2701 catttttatg cgatatattc tatattagca cctttcgagt tgtatcctgg taatcaagtc
     2761 tctggctagc ttttctctcc acccaataaa ttgacatcat gaagaccata tctacagcat
     2821 ccacacaata aaaaaccctc cgaagagggg gaaggggata acaaattaat acgacagtgg
     2881 aatatgtata tattcgtctg ggcgggagat attgatacca tctttaggat caatcgcgtc
     2941 tatgacatag cctcctttca gaatgctacc aattcccagg ctggtaccat tatcaagagc
     3001 aattgttgga ttcttcatcg taccgctgat tctgagtatt tttccgggaa aaatatctgc
     3061 tgaaatgtac ggctgaatgt tctgaaagag tatcttgacg ttactatttt tgttcattgc
     3121 atttataatg gcaaggatct tactctcgtt ctgttgagaa acttcccccg cgacaatgat
     3181 gacatcattc t
//
LOCUS       DAZFJL010000236         3141 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFJL010000236 DAZFJL010000000
VERSION     DAZFJL010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.238"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..110
                     /locus_tag="ACHJ8C_004586"
     CDS             <1..110
                     /locus_tag="ACHJ8C_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554296.1"
                     /translation="LTLGLNALTLTDVTKNAAYGVEIESLVLEINAPAA"
     gene            549..764
                     /locus_tag="ACHJ8C_004587"
     CDS             549..764
                     /locus_tag="ACHJ8C_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5554297.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKVARGE"
     gene            complement(1020..1292)
                     /locus_tag="ACHJ8C_004588"
     CDS             complement(1020..1292)
                     /locus_tag="ACHJ8C_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554298.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            1452..1985
                     /gene="rrrQ"
                     /locus_tag="ACHJ8C_004589"
     CDS             1452..1985
                     /gene="rrrQ"
                     /locus_tag="ACHJ8C_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5554299.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            2206..2319
                     /locus_tag="ACHJ8C_004590"
     CDS             2206..2319
                     /locus_tag="ACHJ8C_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554300.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            2541..2726
                     /locus_tag="ACHJ8C_004591"
     CDS             2541..2726
                     /locus_tag="ACHJ8C_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO5554301.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
BASE COUNT          846 a          598 c          840 g          857 t
ORIGIN      
        1 gtctgacact gggactgaat gcgctgacgc tgacggatgt tacaaaaaat gcagcgtatg
       61 gcgttgagat agaaagtctg gtgctggaga taaatgcacc ggcagcataa taaaaaaaga
      121 gccagcgact gacctgaaag aagacgctgg ctaaaaggcc ttatatgttt gtagagactt
      181 atttttcaca gacagcaatg atgcctgtca atatattatc aatatgcgga ttgtttcagt
      241 tacagatgct ttattaagga aaaaaacagc cagcactgac tttcggtgga gaggtgctgg
      301 ctcagaagga tagttggatt tcacatgata cttatgcctg gcggtatatt ttctgacaga
      361 cagtgacggg tgttgtcaag atattgtgtc atttataacc tgaatcaggg gaggccggaa
      421 tgttatctgg catttttagc agagcctgaa tgccataatc acggctcccg gagttggccg
      481 tcagtgggtg acactggcgg tttttttgtt tttctttact ttcattttct gtcggcggtg
      541 acggagacat acatcagatg gaaaaaatca caacgggtgt gtcatacacc acgtcagcgg
      601 tggggacggg atactggtta ctgcagctgc tggacaaagt ctctccgtcc cagtgggtgg
      661 caatcggtgt gctggggagt ctgctgtttg gcctgctgac gtatctgact aacctttatt
      721 tcaagattaa agaagataag cgtaaggtgg cgcggggaga gtagtcgatg aataaacaat
      781 acgaactggt tgtaaaatga atatttctaa ctgaaaaaac gttccatgag gtaagaaaag
      841 gtcacaggca atcaataaca ggacgtgatg aaagaccctt gcatttgtgc gctttctctt
      901 tagatagcag cagatactga aaatctgagt tgtcggggag tcagggatac agctgtgcaa
      961 gagttggtca ttgtgattcc attgaaatcc tgtatgccat gaagggcagg attttatggc
     1021 tacctgagct ttggtgatag taagttgaaa attcgcattt tttgctgaca tgcgtaacga
     1081 gaatcccata agcagggagg acttaattct tcattaaccc atgcgttgat attatgtttc
     1141 agccgttgaa gcatcagcgg tgttaatgtt gtggtaataa tatccagcgt tttatgtgag
     1201 atcttaccgt aagggtctgc aagaatgctg cttgttgctt cgttattatc tgccatcaga
     1261 agaagtaact ctgatttaac gttttctgtc attagttgta aaaatcttct gcgcaaactt
     1321 tctttactgt tcatttatat ggcttcattt gttgtaatct gctgcgtctc aagggatatg
     1381 tttatgagag cgaccatgag tgttggatta tatacctaac atatcaaggg attagaaatc
     1441 gataaatccc catgaacgaa aaaataaaat acggcctgtc ggctgccgtt ctggcgctga
     1501 ttggtgcagg tgcttctgcg cctgaaatcc tcgaccagtt tctggatgaa aaggaaggta
     1561 accacaccac ggcataccgt gatggtgcgg ggatctggac catctgccgt ggtgccattc
     1621 tggtggatgg taagcctgtt attcctggca tgaagctgtc aaaggaaaaa tgcgaccggg
     1681 ttaatgccat cgaacgtgac aaggcgctgg catgggtgga gaaaaacatc cgggtgccgc
     1741 tgaccgaacc ccagaaagcg gggatcgcgt cattctgtcc gtacaacatt ggccccggta
     1801 agtgcttccc gtcgacgttt tataaacgaa ttaatgcagg cgatcgaaaa ggtgcctgtg
     1861 aggcgattcg ctggtggatt aaggacggtg gcagagactg ccgtatccgt tcaaataatt
     1921 gctacggtca ggtctcacgg cgtgaccagg agagcgcgct ggcgtgctgg gacatcgaca
     1981 gatagcagaa tattttcctg aaaaatgacg ttggccaacg cgggtggata acacgaaatc
     2041 ctgaaaactg gtaaaaccta agtgaataaa agtaaaaacc ccgtttgttg gcagcaagcg
     2101 gggttttgtg ttttctgacc ttgagtaagg caagggagaa attatgggta gggaggtact
     2161 ttccctgtga ggaagtataa aagattcttt ctgaggttgt ccattatgaa aggcattgaa
     2221 gtggagacgc cagccagtct ggatttaaca agagcggcag cttttgccat tcgtattgtg
     2281 gccattgctg ttctggtctg ggcaatccgt tggtggtgat atgaaccgtg ttctgtgcgt
     2341 ggttatcatt gtcctgctgg tggcctgtgg tgcgcttagt ctggggctga atcattaccg
     2401 tgatcacgcc atcatctaca aagagcagcg cgataaaaaa gccagtgagc tggagctggc
     2461 gaacgcgaca attactgata tgcagatacg ccagcgtgat gtcgctgcac ttgatgccag
     2521 atactcgagg gaattagccg atgcgagagc tgaaaatgaa actctgcgtg ctgatgttgc
     2581 cgctggtcgt aagcgcctgc ggatcaacgc cacctgctcc ggtaccgtgc gtgaagccac
     2641 cggcacctcc ggcgtggata atgcaaccgg cccccgactg gcagacaccg ctgaacggga
     2701 ttatttcatc ctcagagaac ggctgatggc aatgcagaag caactggaag gagcacagga
     2761 atatatccgt acccagtgta taccgtgatg ttttgttatg aaggtgttac tggtaacgtt
     2821 aaggtaattt aacaaagagt cagttccgga ctttatagtg tgctcagttc atggccaaaa
     2881 acgatttctg tgataaatat tttgaatatt atttacaggt aaatggagtg gggcacatgg
     2941 atagaaatat tacaatagag aatgaagtat atgcccgtat tgtatgggca gagaaggcaa
     3001 aaacacggta attccgtgtg ttgccatgat acctgattgg cagaatagtt gtttggtttt
     3061 gagtatatag tcagcgtttt ttgttcagta attgctccct caaaaaataa taaaataagg
     3121 tgattatttt tgtttattat t
//
LOCUS       DAZFJL010000237         3121 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFJL010000237 DAZFJL010000000
VERSION     DAZFJL010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.239"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(59..550)
                     /locus_tag="ACHJ8C_004592"
                     /pseudo
     CDS             complement(59..550)
                     /locus_tag="ACHJ8C_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611500.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1248..2329
                     /locus_tag="ACHJ8C_004593"
     CDS             join(1248..1440,1440..2329)
                     /locus_tag="ACHJ8C_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611196.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO5554302.1"
                     /translation="MKLAERIGVAAAARELSLYESQLYAWRSKQQQQMSSSERESELA
                     AENVRLKRQLAEQAEELAILPKGRDILREAPEMKYVSIENHRAEFSIKAMCRVLRVAR
                     SGWYVWLRRRHQMSLRQQFRLTCDAAVHKAFFEAKQRYGAPRLADELPEFNIKTIAAS
                     LRRQGLQAKASRKFSPVSYRAHGLPVLENLLEQDFSASGPNQKWAGDITYLRTDEGWL
                     YLAVVIDLWSRAVIGWSMSPRMTVQLACDALQMALWRRRRPESVIVHTDRGGQYCSGD
                     YQALLKRHNLRGSMSAKGYCYDNACVESFFHSLKVECIHGERFSSREIMRATVFNYIE
                     CDYNRWRRHSACGGLSPEQFENHNLA"
     gene            2899..>3121
                     /locus_tag="ACHJ8C_004594"
     CDS             2899..>3121
                     /locus_tag="ACHJ8C_004594"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554303.1"
                     /translation="MFDLITKTNDNIDEQSVSLSSYRIYKPVIVYTGAQRIFIQCKDT
                     GVSYYAEPDSLILLTKSNHSYNITAIKEFG"
BASE COUNT          875 a          696 c          730 g          820 t
ORIGIN      
        1 ttactttgta acgcagaaaa acatttccta aaatacggtg gacccttttg tggtttcacc
       61 ggtatccctg ccgtcgcaga atctgaagca attttggaaa accatatcat ggataccgtt
      121 ctgcaacagc ctgcagcgct gaaataaccg actcgtcacg cgtcgcattc ggacgataat
      181 gataaaccgt tctgctcaga ttccaggctc cgacaggcct gacggatact aagcccgaag
      241 ctcgttatct gataagtgac cagctcgcgc ttaaaggctg gtttcaaagc ttttttcgat
      301 aacgtctttc aatgcccggt tctcaaggct gatgtcagca aacatctgtt tgagactccc
      361 gttctcgtcc tcaagatcct ttatcttttt aatgtcagag gcttccatac caccgtattt
      421 agatttccaa ttgtagtagg tcgctatcag agatagcggc cttgcggcaa acatccttga
      481 cagttcgtcc ggcttcaacc gacttaatca ctgcgatgat ctgatgctca gtaaaacggg
      541 ctttacgcat aacgatctcc ttcgttggca gaatgagtat gccggaagat ctctaaatgt
      601 gaatggttcg attatgcggg ataatcacaa tacaacggtc accaactatg ccgtttttta
      661 tttttaagac aacatttatt catcttcagc tttccggaat acgtacggaa gcatgcaagg
      721 atttcaataa gaaataaaaa aaacaaaccc aaaaacaata aaaaaacaac acgtcatatg
      781 atgtggcatt gacaaataaa gctcactttt gttgaatgaa tatcgtaaaa ggtataaggc
      841 ctataaactc acaggtatga ctattttcgg cacttcatgt gccgcttttt ttataaaatg
      901 agcctcatat attagacaca ttgctttaac cggcgcattg tagagatgtg atggacggcc
      961 tttcctgaag cacagaagtt aataagaacc gtcacatcca aatcacgcaa tggcatgatt
     1021 gagtatctgc gggtattaaa aacttgccga aaaacagaca tcaaagcctg tacatagatt
     1081 tgtgtaattg cctgattttg atatgttcaa tccagcatca aatgaaggtt aatttatgga
     1141 cgaaaaacag ttacaggctc tggctaacga actggccaaa aacctcaaaa cccctgaaga
     1201 cctcagtcag tttgatcggc tgctgaaaaa gctcaggtaa cgaacccctg aaactcgctg
     1261 aacgcatcgg tgtggccgcc gcagcccgtg aactcagcct gtatgaatct cagctttatg
     1321 cttggcgcag taaacagcag caacaaatga gttcgtcaga gcgcgaaagc gaactggccg
     1381 ctgaaaatgt ccgccttaaa cgacaactgg cggagcaggc tgaggaactg gccatcctcc
     1441 aaaaggccgc gacatacttc gcgaagcgcc tgaaatgaag tatgtctcca tcgaaaatca
     1501 tcgggcagag ttcagcatca aagcgatgtg tcgtgtactt cgggttgccc gcagcggctg
     1561 gtatgtctgg ctcaggcgtc gtcaccagat gagcctgcgc caacagtttc ggctcacctg
     1621 cgatgccgct gtccataagg cattctttga ggcaaagcag cgatacggtg ctccccgcct
     1681 tgctgacgaa ctgccggagt tcaatattaa aaccattgcc gccagcctac gtcgtcaggg
     1741 gctgcaggcg aaagccagcc ggaagttcag cccggtcagc taccgtgcac atggcctgcc
     1801 cgtattggag aatctgctgg agcaggactt cagcgccagt ggcccgaacc agaagtgggc
     1861 gggtgacatc acatacttgc gtaccgatga gggctggttg tatctcgcag tagtcatcga
     1921 cctgtggtca cgcgccgtca ttggctggtc gatgtcaccg cgaatgacag tacaactggc
     1981 ctgtgatgca ctgcaaatgg cgttgtggcg gagaagacgc ccggaaagcg tcattgttca
     2041 tacggaccgt ggtggtcaat actgttcagg ggattatcag gcgctgctga agcgacacaa
     2101 tctgcgtggc agtatgagtg cgaaaggcta ctgttatgac aatgcctgtg tggaaagctt
     2161 ctttcattcg ctgaaggtgg aatgtatcca cggggaacgc tttagcagcc gggaaataat
     2221 gcgggcaacg gtgtttaatt atatcgagtg tgattacaat cgctggcgtc gtcacagtgc
     2281 ctgtggcggt ctcagcccgg aacaatttga aaaccataat ctcgcttagg gtgtccacat
     2341 tacgtgggta ggatcacctc tgcgtcagag cttgttatgc ctgacatgct gtacttcgat
     2401 acggaaatga gaaaacctgg cgtgacgcgc caactgttgt ggatggaata caagccacag
     2461 gcgggtgatc aagcgatggg ataccggaga acccgctcac aactattcca tcgaagcagt
     2521 ccaccacatg cgataacgag actttccctg caggaagtag gaactccgcg atatcgatct
     2581 gacatctcag gttaggttac ccgtcttaaa atccctggaa taaggttatc cggagcctgt
     2641 cttatttctc cgtaatttgt tttgcacatt tgaataacgg gacttatgac gaatgccctt
     2701 aaatgtgaat ggtactatta tgtgtgattg ttacataagt cactattaca gtgtgaagtc
     2761 aatgcaccac aaatgaatta ctgaaactta tagtgaaaaa aataaaacaa aatggcattg
     2821 ctgggatttt tgtgtttttt ttatgatttt ttttgatatt tttgttcaca gttggaaaat
     2881 gagttatgaa ttattagtat gtttgatttg attacaaaga cgaatgataa cattgatgaa
     2941 cagagcgttt cattgagtag ctatagaatt tataagccgg ttattgttta cacaggagct
     3001 caaagaatat ttattcagtg taaagataca ggtgtttcat attatgctga gccagattcg
     3061 ttgatacttc taactaaaag taatcattct tataacataa cagccataaa agaatttggg
     3121 g
//
LOCUS       DAZFJL010000238         3090 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFJL010000238 DAZFJL010000000
VERSION     DAZFJL010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.240"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..237
                     /locus_tag="ACHJ8C_004595"
     CDS             <1..237
                     /locus_tag="ACHJ8C_004595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554304.1"
                     /translation="RKKKLFTLFFFFFYTPSHKNVLYINCQSHHELVSGAPGITPFLP
                     KTANQLSARAMTSLRQDPCGKTRLYAFGFTRNNA"
     gene            351..1089
                     /locus_tag="ACHJ8C_004596"
                     /pseudo
     CDS             351..1089
                     /locus_tag="ACHJ8C_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1368..2345)
                     /locus_tag="ACHJ8C_004597"
     CDS             complement(1368..2345)
                     /locus_tag="ACHJ8C_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO5554305.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVTGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PVSLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
BASE COUNT          781 a          774 c          756 g          779 t
ORIGIN      
        1 agaaaaaaaa aactttttac tctttttttc tttttttttt atactccctc acacaaaaat
       61 gtcctatata taaactgtca atcccaccat gaattagttt ccggcgctcc tggcattacc
      121 ccatttctcc caaaaacggc aaaccagctc tcagcccgcg ccatgactag cttaagacag
      181 gatccctgcg gcaaaacgcg cctttacgca tttggtttta cccgcaataa tgcgtgataa
      241 gcaaacaaaa ggatccgcgc gctgcgattt attgtcgtgg aaggatccgg gcgttcaggt
      301 cagaataact gtatccataa acagtgcgtg tgctacgtga aaaataactc atgaacaatg
      361 tcattcccct gcagaattca ccagaacgcg tctccctgtt acccattgcg ccaggggtgg
      421 attttgcaac agcgctctcc ctcagaagaa tggccacttc cacgggggcc acaccggcct
      481 acctgctggc cccggaagtg agtgcccttc ttttctatat gccggatcag cgtcaccata
      541 tgctgttcgc cactctctgg aataccggaa tgcgtattgg cgaagcccgg atgctgacgc
      601 cggaatcatt tgacctggat ggtgtaagac cgtttgtgcg gatcctgtcc gaaaaagtgc
      661 gtgcacgacg cggacgcccg ccaaaagatg aagtccgcct ggtcccgctg acagatataa
      721 gctatgtcag gcagatggag agctggatga tcaccactcg gccccggcgc cgtgaaccgt
      781 tatgggccgt gacggatgaa acgatgcgga actggctgaa acaggcagtg aagcgggccg
      841 aagctgacgg ggtacatttc gattccggtc acaccacaca ccttccggca cagctatatc
      901 atgcacatgc tctatcaccg ccagccccgg aaagtcatcc aggcactggc tggtcacagg
      961 gatccacgtt cgatggaggt ttataccaga gtgtttgcac tggatatggc tgccacgctg
     1021 gcagtgcctt tcacaggtga cgggcgggat gctgcggaga tcctgcgtac actgcctccc
     1081 ctgaagtaac ggcacttcct tatataagca gtggtcagca gaacagacat aacataagct
     1141 ggagcaggta gataagctgt agtgagtaaa ccatgtttta tgaagggagc aatgcctcag
     1201 catcaggtta cggggtgact cacgtaaggg acaggcagat ggcagctcag ccacaggcaa
     1261 cactgcagga aactgaatat aaactgcagt gagcagacca ctcactgcac ctgataatat
     1321 aagctgtagt cagtaaagga gcactcttca ctgactacag tttatgttca gcgggatttg
     1381 aagagttttt ccaggtcatc cagcgtgatg cccagtttct caagcaaggc aagcttctcc
     1441 gccatctccg gactgacttt ttctgcaggt gaaggtggca acggattttc cttactctca
     1501 tcattcggcg cttttaaccg gggacgccgg tagtgaatac agaagagttt tgttcgcccc
     1561 cgctggatct ccgtgtaatc aagatatcca atctcgcgca actgctccat tgcccgtctg
     1621 accgtctggt tctgggaaaa taccggagac ttcagattga ggcgtgcacg cagccgcgcc
     1681 agcgataccg gtgccggatc ccggggcagg ctctctataa aggtgtagag tgcctgggcg
     1741 gactcccgtc gcttcagggc attaatcgcc ttaagctgga gaaggacttt tctgtcaaac
     1801 tggtacagtt caaacaggcg gggatcggcc tgtaactgaa caatatcccg ctcagtatcg
     1861 tagtaggctg actgtaccag atgggtgatg tattcccggg tgtgcttctc atcggtacgg
     1921 gaaaacgaga tcacggtacc ggcaatgcgc ttcagggaag ggctgatgcg ctcacgcagc
     1981 ctgcgggatg actggcttga aggtatacca cacagttttg caaactcaac aaaaggcagt
     2041 tcaactttgt caccagtcac gttatggcgg gcaaaggaat gaatgatccc cacccaggtc
     2101 ttgaaatcgt tatccatgtc cagacggggg ccggtgatct caaccttatc gaatccctca
     2161 gcccgggcca gggaaagacg cgttagttct tccgtggcat cggtgcgtga cagtgtattt
     2221 tttttactgt tcttcagtga cttaagggtc ggtacaaaga cgcccagacg catcagcgcc
     2281 acaggttgta cggtgttgtt attgtttggt gtcagtgtca ccagctcacc ggacgactta
     2341 tccacctcgc cgaacagttt tttgatgtct gaattttcgt tttccagaaa gcctcctgcc
     2401 tgtggacagt aaaagaataa acagaaaatc gtgtgtcaga accccgtcaa ctgactgaag
     2461 taaaaccttg cagtctgtat ttaacaactg agtctggcgt actgaccata gtttatgtca
     2521 ttaacttact tcagtatatg gattactgaa tacagcttat cattagcatg ttacagttta
     2581 tcagtaacat gccacagctt atgtgatcgt agtcccggcc catgagtgac aaggtttgca
     2641 gcgatatggg gatctttaat ggatcctttt ttgatcattc atggatctgt ttactggatc
     2701 gaacttctgg atatgtggat aaatcatccc agggaaacag catacacaac cgcttataag
     2761 ggctttaggg cgtatctgct cactgtaact acagatacat tgcgcttcct cgccggttcg
     2821 cgtgcttcgc ccggtcaccg gtctgcggcg agcaccgttt ttttttgatt gctataccgg
     2881 agcagaaatc tgatagccaa tccgcaggta acacactgta attgctgtat tttctgtaca
     2941 atatctgttt cactgtgcac cctgctggct cccaaaacac aatggcgcag gaacagaaaa
     3001 taaagaaata ataaaacaaa ttttatacaa aaatatattt tgcatttttt tggtctttta
     3061 ttgcagtttt tgacacaaaa cagaagattg
//
LOCUS       DAZFJL010000239         3006 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFJL010000239 DAZFJL010000000
VERSION     DAZFJL010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.241"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..2868
                     /gene="espC"
                     /locus_tag="ACHJ8C_004598"
     CDS             <1..2868
                     /gene="espC"
                     /locus_tag="ACHJ8C_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_109867760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin EspC"
                     /protein_id="HFO5554306.1"
                     /translation="NFDNDVNIGSGGLIFDAGHHYTVTGNNKTFKGAGLDIGDNTTVD
                     WNVKGVVGDNLHKIGAGTLNVNVSQGNNLKTGDGLVVLNSANAFDNIYMASGHGVVKI
                     NHSAALNQNNDYRGIFFTENGGTLDLNGYDQSFNKIAATDIGALITNSAVQKAVLSVN
                     NQSNYMYHGSVSGNTEINHQFDTQKNNSRLILDGNVDITNDINIKNSQLTMQGHATSH
                     AVFREGGVTCMLPGVICEKDYVSGIQQQENSANKNNNTDYKTNNQVSSFEQPDWENRL
                     FKFKTLNLINSDFIVGRNAIVVGDISANNSTLSLSGKDTKVHIDMYDGKNITGDGFGF
                     RQDIKDGVSVSPESSSYFGNVTLNNHSLLDIGNKFTGGIEAYDSSVSVTSQNAVFDRV
                     GSFVNSSLTLEKGAKLTAQGGIFSTGAVDVKENASLILTGTPSAQKQEYYSPVISTTE
                     GINLGDKASLSVKNMGYLSSDIHAGTTAATINLGDGDAETDSPLFSSLMKGYNAVLSG
                     NITGEQSTVNMNNALWYSDGNSTIGTLKSTGGRVELGGGKDFATLRVKELNANNATFL
                     MHTNNSQADQLNVTNKLLGSNNTVLVDFLNKPASEMNVTLITAPKGSDEKTFTAGTQQ
                     IGFSNVTPVISTEKTDDATKWMLTGYQTVSDAGASKTATDFMASGYKSFLTEVNNLNK
                     RMGDLRDTQGDAGVWARIMNGTGSADGGYSDNYTHVQIGADRKHELDGVDLFTGALLT
                     YTDSNASSHAFSGKTKSVGGGLYASALFDSGAYFDLIGKYLHHDNQYTASFASLGTKD
                     YSSHSWYAGAEVGYRYHLSEESWVEPQMELVYGSVSGKSFSWEDRGMALSMKDKDYNP
                     LIGRTGVDVGRTFSGDDWKITARAGLGYQFDLLANGETVLRDASGEKRFEGEKDSRML
                     MNVGMNAEIKDNMRFGLELEKSAFGKYNVDNAINANFRYSF"
BASE COUNT          885 a          583 c          761 g          777 t
ORIGIN      
        1 aatttcgaca atgacgtaaa cattggctct ggtggtctca tttttgatgc agggcatcat
       61 tatactgtca ctggtaataa taaaacattc aagggtgccg ggctggatat tggtgacaat
      121 actacagtcg actggaatgt gaaaggggtt gtcggtgata acctgcataa aattggtgca
      181 ggtacattga atgttaatgt ttctcaaggt aataatctta aaacggggga tggtcttgtc
      241 gtattaaata gcgctaatgc atttgataat atttatatgg ccagtggtca tggtgttgta
      301 aaaattaatc atagtgcagc gcttaaccag aacaatgact atagaggtat tttctttact
      361 gaaaatggtg gtactctgga tttaaatggt tatgaccaga gttttaataa aattgcagcg
      421 acagatatag gagcactcat aacaaatagt gcagtgcaga aagcagttct ttctgttaat
      481 aatcagtcaa actatatgta tcatggttct gtttcaggta atacagagat aaaccaccag
      541 tttgataccc aaaaaaataa tagtcgcctg attctggacg gtaatgtcga tattacaaat
      601 gacattaaca ttaagaatag ccagctcacc atgcagggac atgctacatc tcatgctgtt
      661 tttagagagg gtggggttac ctgcatgctg ccaggagtta tttgtgaaaa ggattatgtt
      721 tcaggcatac agcaacagga aaactcagcc aataaaaata ataatacaga ttataagacc
      781 aataatcagg tatcatcatt tgagcaacct gactgggaaa atcgtctgtt taagtttaag
      841 acattgaatc tgataaattc agattttatc gttggccgta atgctattgt tgttggtgat
      901 atttctgcca ataattccac tctgtcttta agtggaaaag atacaaaagt acatattgat
      961 atgtatgacg gcaaaaacat cacgggagat ggcttcggtt ttcggcagga tattaaagat
     1021 ggtgtatctg tttctcctga gagcagcagt tattttggaa atgttacgct gaataatcac
     1081 tcattactgg atattggtaa taaatttacc ggtggtatcg aggcttatga cagctccgtg
     1141 agtgtgacct cacagaatgc tgtttttgat cgtgttggca gctttgtcaa cagcagcctg
     1201 accctcgaaa aaggagcaaa actaacggct cagggcggta ttttcagcac cggggctgtg
     1261 gacgtaaaag aaaatgcctc cctgatcctg acggggacac cttctgcaca gaaacaggag
     1321 tattactccc ctgtgatttc tacaacggaa gggattaacc tcggagataa ggccagcctt
     1381 tctgttaaaa acatgggcta tctgagttcg gatattcatg caggaaccac ggcggcaacc
     1441 attaatctgg gagacggtga tgctgagacg gattctccgt tattcagctc cctgatgaag
     1501 ggatataacg cggttctgag tggcaacatt acgggtgagc agagtacggt aaatatgaac
     1561 aatgctctgt ggtactctga cggaaactca acgatcggaa cgctgaagag tacgggggga
     1621 cgagttgaac tggggggcgg gaaagacttt gccaccctgc gggtaaaaga gcttaacgca
     1681 aataacgcca cattcctgat gcataccaac aacagtcagg ctgaccagct gaatgtcacg
     1741 aataaactgt tgggcagtaa taataccgtc ctggtcgact ttttaaacaa gccagccagt
     1801 gaaatgaacg tgacgttaat taccgcaccg aaagggagtg acgagaaaac gttcactgca
     1861 ggaacgcagc agattggttt cagtaatgtc acgccggtaa tcagcacaga aaaaacggat
     1921 gatgccacaa aatggatgct gacagggtat cagaccgtct ctgatgccgg tgcctcgaaa
     1981 accgcaacgg actttatggc gtcaggttat aaatccttcc tgacagaggt caataatctg
     2041 aacaagcgta tgggtgacct gcgggatact cagggggatg ccggcgtctg ggcgcgcatc
     2101 atgaacggta ccggttcggc agatggtggt tacagcgata actacactca cgttcagatt
     2161 ggtgccgaca gaaagcatga gctggacggt gtggatttgt tcacgggtgc attactgacc
     2221 tatacagaca gcaatgcaag cagccacgcc ttcagtggta aaaccaaatc cgtgggggga
     2281 gggttgtacg cttcagcact ctttgattcc ggggcttatt ttgacctgat tggtaaatat
     2341 ctccatcacg acaatcagta cacggcgagt tttgcgtctc ttggtacaaa agactacagc
     2401 tctcattcct ggtatgccgg tgcagaggtc gggtatcgtt accacctgtc ggaagagtcc
     2461 tgggtggagc cacagatgga gctggtttac ggttctgtgt caggaaaatc ttttagctgg
     2521 gaagaccggg gaatggccct gagcatgaaa gacaaggatt ataacccact gattggccgt
     2581 accggtgttg acgtgggaag aaccttctcc ggagacgact ggaaaattac cgcgcgagcc
     2641 gggctgggtt accagttcga cctgctggcg aacggagaaa cggttctgcg ggatgcatcc
     2701 ggagagaaac gttttgaagg tgaaaaggac agcagaatgc tgatgaatgt ggggatgaat
     2761 gcggaaatta aggataatat gcgttttggc ttggagctgg aaaaatcggc gttcgggaaa
     2821 tataacgtgg acaatgcgat aaacgctaac ttccgttatt ctttctgaca gacgtttaaa
     2881 tctggcctgc catctggcag gcctttttat agacacgaaa atctttctca caagaagctc
     2941 gcattttgct ctcataataa aaacaaacaa aagtgaaaca tgtttctcaa aatcaaatta
     3001 cgatga
//
LOCUS       DAZFJL010000240         2988 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFJL010000240 DAZFJL010000000
VERSION     DAZFJL010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.242"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..262)
                     /locus_tag="ACHJ8C_004599"
     CDS             complement(<1..262)
                     /locus_tag="ACHJ8C_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554307.1"
                     /translation="MVRFELIQAKAYIPSSGINQAYLRMDHWNDYSFVTMFYLTVFDE
                     KGIKHDIGNVKIAFKGQTTDTPTYSKLPSSFQKLDDEFFSLGE"
     gene            complement(281..1009)
                     /locus_tag="ACHJ8C_004600"
     CDS             complement(281..1009)
                     /locus_tag="ACHJ8C_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554308.1"
                     /translation="MKFDIPITTLLTTYFTVCGFAYIWGFWYRTGIDTSIVLQILTIS
                     EIIKSAALSFLVFLGAAFATIFSCVMIYTDYKENTFAEVSKNPRAFTFSIFMIASIPL
                     TGGAGIYYIQWALQSGDKFYIANSMGFAAVALLFTGACYKTFVLPNLNAYVRFFTLLT
                     FFLLPPTLFLLGGKSYQQNLTQSKPLYIRNTDNCTSNKNEAFMLLAIYGSKGIGISTS
                     SNALCIFSDSENKYTQVKGLRIHN"
     gene            1457..2590
                     /locus_tag="ACHJ8C_004601"
                     /pseudo
     CDS             1457..2590
                     /locus_tag="ACHJ8C_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          857 a          666 c          654 g          811 t
ORIGIN      
        1 cttctcctaa cgaaaagaat tcatcatcaa gtttttgaaa actgcttggc aattttgaat
       61 aagtaggcgt atctgtcgtt tgacctttaa aggcgatctt tacgttacct atatcatgtt
      121 ttattccctt ttcatcgaaa acagtcaaat agaacatcgt cacgaaagag tagtcattcc
      181 aatgatccat gcgaaggtat gcttgattta ttccgctgga tggtatgtat gcctttgctt
      241 gaataagttc aaaacgaacc atatttactc cttggatttt ttagttgtgt attctcagac
      301 ctttaacctg tgtgtatttg ttttcgctat cactaaaaat acataaggca ttgctagaag
      361 tcgaaatccc aatgcccttt gaaccataaa ttgctaacag cataaatgct tcatttttat
      421 ttgatgtgca attatcagtg ttacgtatgt ataacggttt agattgtgtt aagttttgct
      481 gatatgactt accgcctagt agaaataatg tagggggtag taaaaaaaaa gttaacagcg
      541 taaaaaatct tacataggca ttcaaattag gaagaacaaa tgttttgtaa caagcaccgg
      601 taaacaatag agctacagct gcaaacccca tactgttggc tatgtaaaat ttatctcctg
      661 attgcaacgc ccattgtatg taataaatac cagcgccgcc tgtgagagga attgatgcaa
      721 tcataaatat tgaaaatgtg aatgcacgtg gattttttga tacttcggca aaagtatttt
      781 ctttataatc ggtatatatc ataacgcaac tgaatatcgt cgcaaatgct gccccaagaa
      841 acactaaaaa tgataaggcc gcagacttaa taatttctga tattgtcaat atttgcaata
      901 caatcgaagt atctataccc gttctatacc aaaaccccca aatgtatgca aatccgcaga
      961 ccgtaaaata tgtagtgagt aacgttgtta taggtatgtc gaatttcatg ttggtaattc
     1021 atccataact gtgtttatag gtaattttac ttctcaaata tgagattgag taattttgtc
     1081 ttcaaaaact tctgccagta aacagcaaga agctctctac caaggtcata caatggttga
     1141 actatccctc ttccagcgcg ttcatcatac tcaatccctt gcatgaaatg aacctttagt
     1201 gtttctgggt ctatccccat ttgatgcgta tcgacctact tatggaatgt tggggagagc
     1261 aggattccgc tgtttgcatt gtagcaacca cggaaagctt cttcttcaat gtgttccagt
     1321 tcctgaatat cattcagacg ctattttgtt acagcgcagt ggttatcgaa tgcttttgac
     1381 cctgacccaa aatcctgctc catccagaaa cagaattccg gaatgatgaa tgcttccctg
     1441 agcggaggtg gtgccgatga aaaaatcact attcaccgaa gagcagattg tctttgccct
     1501 gaaacaggcc gatctgggta cgagcgtgcc cgaagtctgc cgtaagctgg gcatctccga
     1561 tgccacgttt tacacctggc gaaaaaaata cggcgtgatt tccccttctg agctgaagca
     1621 catgcggcag cttgaggaag agtatctcag gctgaagaag cttgagcctc tacaaggcca
     1681 tgctgcagga tgtgctggca ataaagaact gacgctagcg tgtctgcgag aatgggtccg
     1741 tgacctgcaa gctcgttacg gggccaacga gcggcagttc tgtttcgcag tagggatcag
     1801 ccgcagctct ttccgctacc gttctgtggc ggcagacgac agcgcactcc gcctacgtat
     1861 cagggagatc acctaaaccc gggtccacta tggctaccgc agggttcacg tcatgctcag
     1921 gtgggaaggc tggcgggata atcataaacg tatttaccgg ctctatcgcg agcagggtct
     1981 gtccctgagc ctcaaacgcc cacgccgaaa tgaatcgaca cagcggcgac agccccagcc
     2041 ccagccccag cctcagcctc agcctcagcc tcagcctcag ggattgtatc caaatcatgt
     2101 ctggggcatg gattttgtct ctgacgcctt atttgacggg cgccggctac gtctgctgac
     2161 ggtcattgat ctctacacgc gcgaatgtct ggggatttgc gtgggtcgaa atctgcgctc
     2221 aacggaggtc gcagagatgc tgaacgccat tgcgctcaga cgtcccttac cacaattgct
     2281 gaaaacccat aatggctctg aatttgcagg aaaaatgctg gacaaatgga tgtacgacag
     2341 agggatccac atcgacctat cacgaccggg aacgcccacg gataatgcca cagtggagtc
     2401 gttcaacggc aggctgcggc aggagtgcct gaacgtaaac tggttcatgt cgctggagga
     2461 tgcacggtgc aaaatcgaag cctagcgcat acactataac cagagacgtc ctcattctgc
     2521 gctgggctgg atgaccccgt ccgaatttgc tgaaaagtct gccggttgcc agaacatgca
     2581 accaacatga agctggttat tcctgattat gactggagca tattcggggt cagggtcaac
     2641 caacccacat tactaactga gagttgtatc taatcctggg gggcaggtca atgatgctaa
     2701 aaaccttgtg gttgacattt ctcaaaagca aattaagaaa caatttgatc aactcaggaa
     2761 ctcacagatg gcgggaatgg tgcctaatga tgcagataat attcagatag agactattaa
     2821 tgtcagaact ataaaacatg actcctcact agattcctaa ttttgctcag ccgacttaaa
     2881 aatgacttta cccgagaaaa taaatttaac accggtaaca tataacatcc agaaaacaga
     2941 tggtgataat ggacagtttt acattaactt atttggattg taatatta
//
LOCUS       DAZFJL010000241         2975 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFJL010000241 DAZFJL010000000
VERSION     DAZFJL010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.243"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            546..776
                     /locus_tag="ACHJ8C_004602"
     CDS             546..776
                     /locus_tag="ACHJ8C_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554309.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            828..2189
                     /locus_tag="ACHJ8C_004603"
     CDS             828..2189
                     /locus_tag="ACHJ8C_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFO5554310.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQRQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            2237..2800
                     /locus_tag="ACHJ8C_004604"
     CDS             2237..2800
                     /locus_tag="ACHJ8C_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5554311.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVM
                     LAEISNRDHILEPSAGTGAILRAIRDTAPGAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPAQYYSRVIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(2886..>2975)
                     /locus_tag="ACHJ8C_004605"
     CDS             complement(2886..>2975)
                     /locus_tag="ACHJ8C_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
                     /protein_id="HFO5554312.1"
                     /translation="NNNISNRQMTGMKKQKPPSERPRNERGGA"
BASE COUNT          695 a          818 c          873 g          589 t
ORIGIN      
        1 tccgtcgttg cggcgggtcc ctgctcattg cctgccggtg ccacacaccc gtatgagtcc
       61 ctgtcgccgg gggctgatac agccacaacc tgccgcactg actgtccagg gtaaatgaac
      121 agcccacaaa attttttacc caaaacccgg taaaaaattt tgcgttctgc ccaggacagg
      181 tgcgtcaggc cgtggcagtg atgccccttg cggcgaaggt ttctcaaacg ggtgaagttc
      241 ataccacagg gaatacagca gcaggttccc gcgaaacgtc aaaaccgcgg ctcccgaaac
      301 tcaacgggtg atgttcttta aaaatcagga tttatttcag gctccggcaa tacgcattta
      361 tgtgttctgt cagtcatccg gctggcaggt aatgagaacg caggaacaga aggaggaaaa
      421 caccggagaa cgtcagacgc agatatgaaa aaagcaggtc agggacctgc ttaatccacg
      481 ccgtaagaat tgcgcaacca attttgacgg cattgttaac caatttgtca taaggagttt
      541 catttatgac agtaagtatt gtttcacctt ccgcagcggc tgtaaagccc cgccgtcatc
      601 cccgtttcag acgcgaagac atccccgcgc cggaaattga ccccgtgctg aaagccttcg
      661 ggcgacacat tgcccggagt tttcaccggg ggcgtggtgt acacatcccc gccatgaaaa
      721 acacggcctt cgggcaggtg ctccgcacac tcgaactgaa acgcgctttt aactgatgag
      781 gtgagccgcc ccgcgagggg cggcaacaga cggaaggaga acattttatg acattagcag
      841 ccatcaccat gaccgccccg gaagccgcca gccctgtgca gatgtaccgc gcgacctact
      901 caccggatga caacaaactg cgcctgtatg ccgcgtcacg cctcgacccg gagacgtata
      961 aaaaagtgca tgatgctggt tttcgctggg cacccaaaca ggcgctgttt gtcgcgccgg
     1021 cctggacacc gggccgggaa gacgtgctcc tctcacttgc cggagagatt gaggatgaag
     1081 acagcacgct cgctgaacgt caggaagcac gggcggagcg gtttaccgga tacagcggaa
     1141 agcgggccag tgaatccgca caggcacttg atgaagtgga aagactggcc gcgatgatcc
     1201 cgcccggtca gcccattctt gtggggcatc acagcgaacg ccgcgcccgt cgtgatgcgc
     1261 agcgtattga aaacggcatg aaacgtgccg tgatgctctt tgaacgtgcg gaatactggg
     1321 aagaacgcgc gcggtcggca ctgcttcacg cgaagtataa agaacgtccg gacgttcgct
     1381 ggcgtcgtat caaaaaaatc gaagctgatt tgcgcaaggc tgaaaagacc attgcacagt
     1441 cgcagaaata tctgacgatg tggcgggctg aatcgctgga tctgaatatg gcaaaactca
     1501 tcagcagtca tgaccatatt agcgcctgtt tcccgctgga tacgtatccg cgcccggcag
     1561 aaaaaagcca gtatgaagga agtcgatcgt tatggtcggc cctggatgat gacatcatca
     1621 ccacggagca ggcccgcgaa attgcgatcc gctgtcatga acggcagatc cagcgtcagc
     1681 aacgctgggt taaccactat cagaaccgcc tgatctatga gcgtgccatg ctggacgaaa
     1741 gcggcggcgt ggttacccgg acacaggatt ttgagccggg cggacaggtt ttcagccggg
     1801 gcgagtggct gaccatcatc cgtgtgaaca aaagcaacgg ggcggtgagt tcagttacaa
     1861 cgccaaatta cagttttctc gggtacagcg gcacgatgaa agtgacgccc gatcgcatca
     1921 cggactacaa agcaccatcg gcagaagagg ctgccgtcgc cagtcaggcc gcgaagcgtc
     1981 cgccggtagt caactatccg ggggaaggtt tccgggaaat gacaaaggca cagtgggccg
     2041 ccctgccccg ggactgtaag gccgtgcgca gtgtggcaga agcagaagac cacggggcat
     2101 accgctaccg ccgcacaatg gacaataatt tccgcctggt gaatgtgtat atcaccgaca
     2161 tgaaaatcac ggaaattcca cagaaataag gtatatcccc ccgggaaaat ccccggggga
     2221 tgacggagat aatgacatgc acagtcagtt aagagaacgt atccggctga tgcgcgcaag
     2281 gctggataac gccgcgccgg ttgctgaaat ccgggctgaa tctcagcttt ttgtgactcc
     2341 tgccccggtc tgtgatcgcc tggtgatgct ggcggagatc agcaaccgtg atcacattct
     2401 ggaaccctct gccggcaccg gggccatact gcgggcgatt cgggataccg cgccgggggc
     2461 catgtgtgat gcggtggaaa tcaacagcgg gctggtccgg tatctgcggg aaaattttaa
     2521 cggtgtcagg gttcagtgtg gcgacttcat ggaatggcag ccggcgcagt attacagccg
     2581 ggttatcatg aatccgccgt tcagccacgg gcaggatatc cggcatatcc tgcgggcctt
     2641 ttccctgttg cgtccgggtg gcgtgctggt cgccgtctgt ctcaacggac cacgccagca
     2701 ggagaagctg ttaccgtttt ctgacgtccg cgaggagctg ccgcgcggca cgtttgctta
     2761 taccgatgtc ccgacgatga ttattcgtct gcgtgcctga agacgccggg caggcgggag
     2821 gataaatgcc cgtctgcccg cgccgggaaa agaccgcgcg ccgatggcgc tgaaaggggc
     2881 cgcgctcacg cgccgcccct ttcattacgc ggacgttctg atgggggctt ctgtttcttc
     2941 atgcctgtca tttgtctgtt actgatgttg ttgtt
//
LOCUS       DAZFJL010000242         2883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFJL010000242 DAZFJL010000000
VERSION     DAZFJL010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.244"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..2081
                     /locus_tag="ACHJ8C_004606"
     CDS             <1..2081
                     /locus_tag="ACHJ8C_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5554313.1"
                     /translation="SLRELTLIIGREKFAEIAQGYLEAMAGHDFVSEQQLHALCADMR
                     NGGLDALYLLLSDGLVMQDFMAYRSIFHEGSMTVNDNDFLKAVGQDLSCATSNGEYFI
                     DDENRVISELVEHNRIYADGALHHQLVTHLIDNADEHLAGMFASLFSQSDEHILAVFS
                     ELYTRFGKPETFDKLIVTALQKNGYLDRMVAVLAGQLGEKFTNDIAISVIACVSSEHA
                     LDKLQYRQLIHSLGCRIISQLTEENQEAFMRHVQQADACYDELFEPITPTERYCLQFI
                     AQNSLYQLTRHNVGIAISCLIDNITPEEAERKPWTLAYEHKLSTVSDYFSQNIDIFVR
                     DVFISSSEDADCIRYVLTRTSLSDDSKGDIVRNMIFSFDDLSGISAKAEFTEDQLTIS
                     YHDLFYRYDRIAPGWGALIDYICQDCDMEILTTYVTKHAPVLGQSSPEVYDGDRYDLL
                     YMKIICNDDLDEWTYQNLVSPLEINMREIDDRLSARNFCTLIAMLKLRLDSDVYEKIA
                     AQYADLDEKISDAFVYWFSEYKSEFLAQPEFYLRKEKDARFFTVMLTKVMAYSRFTVQ
                     ERADLVSLFIDYFIASDIADPNFPNDVLLQVFNSTSNEEFKVMLFTRFIVTGLNKHQL
                     AGLCHQLGEDELRNVFINRTQATIAVANRERVISILEHLQAVRIIRSFQEREGGKISV
                     FIEPDLEDDD"
     gene            2314..2508
                     /locus_tag="ACHJ8C_004607"
                     /pseudo
     CDS             2314..2508
                     /locus_tag="ACHJ8C_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010306206.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            2508..>2883
                     /locus_tag="ACHJ8C_004608"
     CDS             2508..>2883
                     /locus_tag="ACHJ8C_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HFO5554314.1"
                     /translation="MKTKSSTIGAVSTFIERIMDFSFENTSPTAFRGHKNDTWKAIPS
                     IFRDEYADAYNNENSIVRDIVSAHPAEFESDKTMFDRLVRMQHYSLPTRLLDVTINPL
                     VALWFATEDNEDKVYLPNGRVRN"
BASE COUNT          770 a          613 c          688 g          812 t
ORIGIN      
        1 tttcgctgcg agagcttacg ctaatcatcg ggcgtgaaaa atttgcagag attgcgcagg
       61 ggtatcttga ggcgatggcg ggtcatgact ttgtctctga gcagcagctc catgctttgt
      121 gtgccgatat gcgcaatggc ggcttagacg ccttgtatct gctgctgtcc gatggtctgg
      181 tgatgcagga cttcatggcc taccgctcta ttttccatga aggatcgatg accgtaaatg
      241 ataacgactt tctgaaagcc gtcggtcagg atctgagctg cgccacctca aacggtgaat
      301 attttattga tgacgagaac agagtcatca gcgaactggt tgaacataac cgtatttatg
      361 ccgatggcgc gcttcatcat caactggtga ctcatctgat tgataacgcg gacgaacatc
      421 tggcgggcat gttcgcttcg ctattcagcc agtctgatga acatatcctt gctgttttct
      481 ctgagcttta tacacgattt ggcaaacctg aaaccttcga taagttaatc gttaccgcgc
      541 tgcaaaaaaa tggttatctt gacagaatgg ttgccgttct cgcgggccag ctgggtgaaa
      601 aattcaccaa cgatattgct atcagcgtga tagcctgcgt atcgtctgaa catgcgctag
      661 ataaactgca ataccgccag cttattcact cgctggggtg ccgcatcatc tctcagctca
      721 ccgaagaaaa tcaggaagcg ttcatgcgtc atgtacaaca ggcggatgca tgctatgacg
      781 agctgttcga accaataacg ccaacggagc gatattgcct gcagtttatt gcgcaaaaca
      841 gtctttatca gttaactcga cacaatgttg gcattgccat ttcatgtctt attgacaaca
      901 taacgcctga agaggcggaa aggaaaccct ggacgctcgc ctatgagcat aaactcagca
      961 cggtttctga ttacttcagt caaaacatcg atatattcgt cagggatgtg tttatctcct
     1021 cttcagagga tgccgactgt atccgctacg ttctgactcg cacctcccta agcgacgatt
     1081 ctaaaggtga tattgtcaga aacatgatct tctcgtttga cgacctgtcc gggatatcgg
     1141 ctaaagcgga atttaccgaa gaccagctaa cgatttccta tcacgacctg ttttaccgtt
     1201 atgaccgcat cgcaccggga tggggggcgc tgatagacta tatctgccaa gactgtgaca
     1261 tggagatact gacgacttat gtgacaaagc acgcgcctgt attggggcag tcatccccgg
     1321 aagtttatga tggcgaccgc tatgacctgc tgtatatgaa aattatctgc aacgatgacc
     1381 tggatgaatg gacttatcaa aatcttgttt caccgcttga gattaacatg cgggaaattg
     1441 atgatcggct cagcgcgcgt aatttctgca ccttgatagc catgctgaaa ctgcgccttg
     1501 attcagatgt gtatgaaaaa attgcggctc aatacgcgga tctggatgag aaaatcagcg
     1561 atgccttcgt ctattggttc agcgagtata aaagcgaatt tttggcgcag cccgagttct
     1621 atctaagaaa agaaaaagac gcgcgctttt ttacagtcat gcttacaaaa gtcatggcct
     1681 actcgcgctt tactgtgcaa gagagagcgg atctggtgtc gctatttatt gattatttca
     1741 ttgcgtctga tatagccgat cctaatttcc ctaatgatgt cctgctgcag gtgtttaaca
     1801 gcaccagtaa tgaagaattc aaagtcatgc tgtttacgcg atttattgta acaggcctga
     1861 ataaacatca gctggcaggg ctttgtcatc agctgggcga agatgaattg aggaatgtat
     1921 tcatcaatcg aacccaggcg acaattgctg ttgcaaacag ggaacgcgtg atatcaattc
     1981 ttgagcatct gcaggcagta cgcatcatca gaagtttcca ggagcgtgag ggtggtaaaa
     2041 tctccgtttt tatcgaaccc gatctggagg atgacgatta gcgatttagc tttttcaggc
     2101 tggggtcggc aggtctctgc ttgtctttgt taatctatta acgactctat acatgttaga
     2161 ttctttcagc aatatgttgc tgaacagtcc gctcctggca cgaagcagac gagttaactg
     2221 aactgaaggt ccactgtgag cgaggagcgg aagttggcag ttgatctcgc agctaaatag
     2281 tcgcattact aacgttgcgg gatgttaatc aacggggagc aggtcaatgt gttgcgccat
     2341 acgtttgcga gtcattttat gatgaatgct ggggatatgc ttgtgttaca aagaataata
     2401 ggacactcta atataagaga aacaatgcgt tactcacatt cttctcctga gcatcttcca
     2461 gatgctgtat tgtttaaccc tcttgccaat tatcatgggg aatattgatg aaaacgaaga
     2521 gttcaacgat tggagcagta agtactttca ttgaaaggat tatggatttt agttttgaaa
     2581 atacctcacc aacagcattt agagggcata agaatgatac atggaaggcg atcccttcta
     2641 tctttagaga tgagtatgcc gatgcttata ataatgagaa ctctatagtt cgtgatattg
     2701 tttcggctca tcctgccgag tttgagtcag acaaaactat gtttgatcga ctcgttagaa
     2761 tgcaacatta ttcattgccc acgcgacttt tggatgtgac tattaatcct ttagttgctt
     2821 tgtggtttgc caccgaagat aatgaagata aagtatattt acctaatggt agggtaagaa
     2881 ata
//
LOCUS       DAZFJL010000243         2878 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZFJL010000243 DAZFJL010000000
VERSION     DAZFJL010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.245"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(364..1218)
                     /gene="rclR"
                     /locus_tag="ACHJ8C_004609"
     CDS             complement(364..1218)
                     /gene="rclR"
                     /locus_tag="ACHJ8C_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO5554315.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMMEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            1445..2770
                     /gene="rclA"
                     /locus_tag="ACHJ8C_004610"
     CDS             1445..2770
                     /gene="rclA"
                     /locus_tag="ACHJ8C_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO5554316.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPGGNLEIHGEKIFINTGAQAVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFTSMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTANNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT          833 a          623 c          640 g          782 t
ORIGIN      
        1 actaatttta tttctttcgg catcgctcac attttatagg gaaatcgacg atcatttttg
       61 gcataagaaa tccatttaaa aaggctacat cccttatacc tattcagagc tactcattca
      121 ggggttattg agatatacac tgatggtgta caaaaaaaat caccgacaat aatcacgtat
      181 caagtaatag tcgggatttt atttaattga agaaatttag tttgcatttt tatttaaaaa
      241 ctgagctaga gtccatcaaa aaaatatata aaacccaaaa ccaccgttgg aaatagaaat
      301 agttgaaagg ctcacaactc ccggaatgcg gattatcttt gccctgattt ctggcggttt
      361 tattcagggt gcaagctgtc tcaccctttc ccgatattct cccggagtac aaccaaactc
      421 gcggacaaac gccttgtgaa aagatgattc gctggcatag cctactgact cagcgatcac
      481 cacaacaggg agcatttccc gggaaaacat ctgggccgct atttgtagac gcaactttgt
      541 taatacagcc agcggcgtgg ttccggaaac atcacggaaa agctgggcaa aacttgcccg
      601 ggacatgtgg gcgatgctgg ccagcgattc gacggtccag gcgtgtccag gcatttccat
      661 catttgctgt attaccgcac caagacgtgg atgcagaagc aaactgagaa tgtttttctc
      721 tgtattaacc tgtgcaatcc attcacgcac cgcgagggta aagaatgtag cgcagatctg
      781 gctacacaga gcatccactc ccggcattgc cgatctggat tcctgttgta aaaacgggat
      841 cgcctccctc agccagttat attccacgct gtggttaacc ggtgctacaa acagcgtttc
      901 cggcaaagac gttaaaaaat aacgcgccga atgttgcagc cgaagagttc cacagacaat
      961 acaggtcgat tcattatcga catgactcag gcgatgagct gaattttgtg gtagcaggac
     1021 cacatttccc gggcgtaatg taaaaatctc tcccgtcggc atttccagct tcgccgctcc
     1081 ttgcgttaac gcatgccaac gaataaccga caattccccg gcaccatgtg gaagttgcca
     1141 gtcacttcct aacacgcaat tcttatcgat cgttccttgt ggagcgttaa gcatcaacaa
     1201 acggctaaga gcatccatat cagactcctg agtggttttt tgattacaaa caccaacaat
     1261 cttagtcaat aattaatcta ataccatcaa atagttaatc atgatttttg ttttgtgatt
     1321 ctgattaaga aaaagaaaat accagacgat aagacaaaat atcaagcgtt gtgaagaaat
     1381 gttattttct cttttgcgtc tatagtcatg atgtcaaatg aacgcgtttc gacaggaaat
     1441 catcatgaat aaatatcagg cagttattat tggttttggc aaggctggaa aaacattagc
     1501 cgtcacgctg gcaaaagcag gttggcgtgt ggctcttatc gaacaatcaa atgcaatgta
     1561 tggcgggacc tgtattaata tcggctgtat cccaaccaaa acattggttc atgacgcaca
     1621 gcagcacaca gattttgtcc gtgccataca gcgtaaaaat gaagtggtta attttttacg
     1681 taataagaat tttcataatc ttgcggatat gcccaatatc gacgtgatcg acggccaggc
     1741 ggagtttatc aataatcata gcctgcgtgt tcatcggcct gggggaaatc tggagattca
     1801 tggcgagaaa atttttatta ataccggtgc acaagccgtg gttccgccaa ttcctggaat
     1861 taccaccacg cctggtgtat atgacagcac cggattactt aatctaaaag aattgcctgg
     1921 gcatttaggt attttgggcg gcggatatat tggcgttgag ttcacctcta tgttcgctaa
     1981 ttttggcagc aaagtaacca ttttagaggc agcttcgctg tttttgcctc gggaagatcg
     2041 ggatattgct gataatatcg cgacgatttt acgcgatcag ggcgtcgata taatcctcaa
     2101 tgcccatgtg gagcgaatca gtcaccatga aaatcaagtg caagtgcata gcgagcacgc
     2161 ccaactggcg gtggatgcac tgttaatagc ttccggtcgt caaccggcta ccgcttcgtt
     2221 acatccagaa aatgcaggta tcgcagtaaa cgagcgcggg gcaattgtcg ttgacaagca
     2281 attacatacc accgcaaaca atatttgggc gatgggagat gttaccggcg ggctgcaatt
     2341 tacttacatt tcactggatg attaccgcat tgtgcgtgat gaattgctgg gagaaggcag
     2401 acgtagtact gatgatcgga aaaatgtgcc ttattccgta ttcatgacac cgccactgtc
     2461 cagggttggt atgacagaag aacaggccag agagagtggt gctgatattc aggtggtgac
     2521 attgcctgta gctgcaattc cgcgtgccag agtgatgaat gatactcgtg gggtattaaa
     2581 agcgattgtt gataataaaa cccaacgtat attaggggca tcactgctgt gtgttgactc
     2641 ccacgagatg atcaatatag tgaaaatggt gatggatgcc gggctgcctt atagcatatt
     2701 acgcgatcag atatttactc atccgtcgat gagcgaatca ctcaatgatc tattttcatt
     2761 agtcaaataa actcaaaatc agacgccaga acaaatattc tggcgtctca gagaaaagaa
     2821 tcttattaat tccctgttac tttataactt cttaatcact tcattgatgg tattttat
//
LOCUS       DAZFJL010000244         2833 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZFJL010000244 DAZFJL010000000
VERSION     DAZFJL010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.246"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            138..2417
                     /gene="ydeP"
                     /locus_tag="ACHJ8C_004611"
     CDS             138..2417
                     /gene="ydeP"
                     /locus_tag="ACHJ8C_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO5554317.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDDVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTIGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLDRLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTSSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKSSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2665..>2833
                     /gene="safA"
                     /locus_tag="ACHJ8C_004612"
     CDS             2665..>2833
                     /gene="safA"
                     /locus_tag="ACHJ8C_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO5554318.1"
                     /translation="MHATIVKNKITQRDNYKGIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLT"
BASE COUNT          774 a          653 c          684 g          722 t
ORIGIN      
        1 taacgataat agagtaaaaa tagtaagaga gtagtcccct actcttatat attcatgtta
       61 acgataaacc ctttgtatat actttgcatc atcgctatta caaatcctaa taattcattt
      121 ccacacagga taagcagatg aagaaaaaaa ttgaatccta ccagggagct gccggtggtt
      181 ggggtgctgt taaatccgta gcgaatgcag tacgtaagca gatggatata cgccaggatg
      241 ttattgccat gtttgacatg aataagccag agggctttga ctgtccggga tgtgcatggc
      301 cagatcctaa gcacagtgcg tcattcgaca tttgtgaaaa cggcgcaaaa gcaatcgcct
      361 gggaagtcac ggataagcag gtaaatgcct ctttctttgc tgagaatacg gttcaatcat
      421 tacttacctg gggagatcac gagcttgagg ctgcgggacg tctcactcag cctttgaaat
      481 atgatgacgt cagcgactgt tacaagccat taagctggca acaagctttc gacgaaattg
      541 gcgctcgcct tcaaagctat agtgatccca atcaggttga attctatact tcggggcgca
      601 cttccaatga agctgccttt ctttatcagc tttttgcccg tgaatacggg agcaataact
      661 ttcccgactg ctccaacatg tgccatgaac cgaccagtgt gggattggca gcgagtatcg
      721 gtgtgggtaa agggaccgtg ttgctggaag actttgagaa atgcgattta gtcatttgca
      781 ttgggcataa ccctggtaca aaccaccctc gcatgctgac ttcgttgcgc gctttagtga
      841 aacggggagc gaaaatgatc gccatcaatc ctctacagga acgtggcctg gagcgattta
      901 ccgcaccgca aaacccgttt gaaatgctga cgaactctga gactcagttg gccagtgcct
      961 actataacgt gcgcattggt ggcgatatgg cgttgctcaa ggggatgatg cgcctgttaa
     1021 ttgagcgcga tgatgctgca agcgccgcag gtcggccctc attactggat gacgaattta
     1081 ttcaaacgca taccatcggc tttgacgagc tacgccgtga cgtgctcaat tccgagtgga
     1141 aagatatcga acgtatttct ggactaagtc agacacaaat cgccgaactt gctgatgcat
     1201 atgccgctgc cgaacgaacc attatctgtt acggaatggg gatcactcag cacgaacatg
     1261 gtacccagaa cgtacagcaa ctggtcaatc tgctgttgat gaaaggtaac attggcaagc
     1321 ctggtgcggg tatctgccca ctacgaggtc actctaatgt acagggcgac cgaaccgtcg
     1381 gtatcaccga gaaaccgtct gcagagtttc tggatcgcct gggtgagcgc tatggcttca
     1441 ccccacctca tgcacctgga catgctgcaa tagccagcat gcaagctata tgtacggggc
     1501 aagctcgagc attgatctgc atggggggca actttgcact ggcaatgcca gatcgggaag
     1561 cgagcgctgt accgttaacg caattagatc tggcggtaca cgtagccact aagcttaacc
     1621 gctctcatct gctgaccgca cggcatagct atattctgcc ggttctggga cgtagcgaga
     1681 ttgacatgca aaaaagcggt gcgcaggcgg taaccgttga agattcaatg tcgatgattc
     1741 atgcctcgcg tggtgtgtta aaacccgccg gtgtaatgct gaaatcagag tgtgcagtgg
     1801 tcgcgggaat cgcgcaagca gcactacccc agagcgtggt agcctgggag tatctggtgg
     1861 aagattatga tcgcattcgc aatgacattg aagctgtgct gccagagttt gccgactata
     1921 accagcgcat ccgtcatcct ggtggttttc acctgataaa cgcagcagct gaaaggcgct
     1981 ggatgacgtc gtcaggtaag gctaatttca ttaccagtaa agggctgtta gaagatccct
     2041 cttcagcgtt taacagtaag ctggtcatgg cgacagtacg cagtcacgat cagtacaaca
     2101 cgacgattta tggtatggat gatcgctatc gaggggtatt cggtcaacga gatgtggtct
     2161 ttatgagtgc taaacaagct aaaatttgcc gtgtaaaaaa cggcgaaaga gttaatctta
     2221 ttgcactcac gccagacggt aaaagtagct cacgccgcat ggatagatta aaagtggtca
     2281 tttaccctat ggctgaccgc tcactggtga cctattttcc agaatcgaat cacatgctaa
     2341 cacttgataa ccacgatcca ttaagtggca ttcctggcta taaaagtatt ccggttgaat
     2401 tagaaccatc aaattaattc gcctcctcat ttcttctgct gtcatccgca cagcagaaga
     2461 attcctcatt gactattatc gcgcaatttg ctcacatgga ttaaattaaa ccacacacta
     2521 taagatataa acttctgcct acagctgtaa gaaactccgc tcagtactga agtacccgtc
     2581 ctatttcctc tattctccag cctgttatat taagcatact gattaacgat gcttaacgtc
     2641 attcgtttaa taaacacatt tgaaatgcat gcgaccatag tgaaaaataa aatcacgcaa
     2701 agagacaact ataaagggat catgtctgta attgtggttg tcttattact gacccttaca
     2761 ttgatagcca ttttttcggc aattgatcag ctgggtattt cagaaatggg tcgcattgca
     2821 agggatctta cac
//
LOCUS       DAZFJL010000245         2789 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFJL010000245 DAZFJL010000000
VERSION     DAZFJL010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.247"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            55..687
                     /gene="comR"
                     /locus_tag="ACHJ8C_004613"
     CDS             55..687
                     /gene="comR"
                     /locus_tag="ACHJ8C_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO5554319.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELVK"
     gene            complement(749..1288)
                     /locus_tag="ACHJ8C_004614"
     CDS             complement(749..1288)
                     /locus_tag="ACHJ8C_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO5554320.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMERD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1515..>2789)
                     /gene="ndh"
                     /locus_tag="ACHJ8C_004615"
     CDS             complement(1515..>2789)
                     /gene="ndh"
                     /locus_tag="ACHJ8C_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO5554321.1"
                     /translation="VGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHSHLWKPLLHEV
                     ATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDEKGELLVPERK
                     IAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLKYSANLGANGK
                     VNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGERILPALPPRI
                     SAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGIKAPDFLKDIG
                     GLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAAHQMATCAMNN
                     ILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRIARFVYISLYR
                     MHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          663 a          770 c          689 g          667 t
ORIGIN      
        1 ggtgattatt tttataataa cctttcattc acagtcacca ggtacaaagg catcatggca
       61 actgactcaa cacaatgtgt aaaaaaaagc cgtggccgcc caaaagtgtt cgacagggat
      121 gccgcgcttg ataaggccat gaaattgttc tggcaacacg gttatgaagc gacttctctt
      181 gcggacctcg tcgaagcgac cggagccaaa gcgcccacgc tatacgcgga atttaccaac
      241 aaagaggggt tattccgcgc cgtactcgac cgctatatcg atcgttttgc cgctaagcat
      301 gaagcacagc tgttttgtga agagaaaagc gtggagtctg cgctggctga ctattttgct
      361 gccatcgcca actgctttac cagcaaagac accccggcag gctgcttcat gatcaacaac
      421 tgcaccaccc tctccccaga ttcaggagat atcgccaata cgctgaaatc acgccatgcg
      481 atgcaagagc gcactttgca gcagttttta tgtcaacgac aagcgcgcgg ggaaatcccg
      541 acccactgtg acgtgacgca tctggcagaa ttccttaatt gtattattca ggggatgtcg
      601 atcagcgcac gcgaaggtgc atcgctggaa aaactgatgc agattgcccg aacgactttg
      661 cgtttatggc ccgaactggt gaaataaggc gcaaaaaaag cccctcagcc tgagcgaatg
      721 aggggccaaa ttgcagagta cagccttgtt atactttgtt attcaaaatc agttgcccgt
      781 tgctatctag cgggatctgc gtacccggat cgcgttccat gcggattttg ccctgctgat
      841 cgccaatctt ataggtcaca tcataaccga gcattttttc tgacttgtca tacaccgttt
      901 tacaacgctg ttgcgtagtc gtgtaagtat cgctttcctg gagagagccc tggatctggt
      961 taccggcata tccaccaccc agcgccccca caacagtagc gacatcttta ccgcgaccac
     1021 caccaaactg atgccctatc acgccaccag caacagcgcc gagcaccgac ccggtaatgc
     1081 gattttcatc ctgcaccggt cgacgatggg tcactgtgac gttgcgacac tcctgacgcg
     1141 gtgttttaac cgtttccttg attggggttg cagaaacaac ctgagcgtat tgcgggccac
     1201 gttcaaacac gttcagactg gccactgccg ctacgcccaa cgcagctgcg acaccaatcc
     1261 cgatacccgc caacattgat ttattcacgg gaatacctcc ttctttgctg ttatgccaat
     1321 catgcaagcg aaggagaaaa gcagctatca gacagaagat gaaaagcgcg ggaatggctg
     1381 gaaatactta gggattcgga ggagtctgaa ggaatgcctg atgcgacgct taacgcgtct
     1441 tatcaggcct accgctcgta ccatttgtcc agggcggata aggcgtttac gccgcatccg
     1501 ccagtacaag ccgattaatg caacttcaaa cgtggacgga taacgcggtt aatactcccc
     1561 accagcatca ttaatccggt tttaaagtaa ccatgcagcg caatctgatg cattcggtat
     1621 agcgagatgt atacaaagcg cgcaattcgt ccttcaatca tcattgagcc gcgcgtcagg
     1681 ttacccatca ggctaccgac ggtggagaag ttcgacagcg ataccagcga accatgatct
     1741 ttatactgat aatttttcag cggcttaccg ttcatctgcg ccagaatgtt gttcattgcg
     1801 caggttgcca tctggtgtgc ggcctgcgca cgcggcggaa caaagccccc ttccggacgc
     1861 gggcatgacg cgcagtcgcc aatagcgtat atgtctggat cgcgagtggt ttgcagcgtc
     1921 ggttccacca ccagctggtt gatacggttg gtttcaagac caccgatatc tttcaggaag
     1981 tctggcgctt tgatcccggc tgcccacacc atcagatcag cctcaatata ttcgccatct
     2041 ttagtatgta ggccgccttc atcagcactg gtgaccatgg tttgcgtcag cacgcgaacg
     2101 ccaagtttcg ttaactcatt atgggccgca gcagagatac gtggcggtaa tgcaggcaaa
     2161 atacgttcgc ccgcttctac cagcgttacg ttcagggctt cgttggtcag acctttatag
     2221 ccgtagctgt gcagttgctt gaccgcgttg tgcaattcag cggagagttc tacacccgtc
     2281 gcgccgccgc cgactatcgc aatgttcact ttgccattcg cgcccaggtt ggcggagtat
     2341 ttcaggaaca gattcagcat ctcctggtgg aagcgacgcg cctggtgcgg gttatcgagg
     2401 aaaatgcagt tctctttgac acctggcgta ttgaaatcgt tggaggtgct acccagcgcc
     2461 attaccaggg tgtcataggc gattttacgt tccggaacca gcaattcacc tttttcgtcg
     2521 cgcagttctg caatagtgat tgttttcgct tcacgatcaa tatcaattac ggaacccagc
     2581 tggaactgga aaccatgatt gcgcgcatgg gccagatagc tcaacgcatc gacgccttca
     2641 tcaagcgagc cagtcgccac ttcgtgcagc agcggtttcc acaggtggct gtggttacga
     2701 tcgaccagcg taattttggc ttttttcttg cgtcccagct tatgccccag ctgtgttgcc
     2761 atttccagcc caccagcacc gccgccgac
//
LOCUS       DAZFJL010000246         2773 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZFJL010000246 DAZFJL010000000
VERSION     DAZFJL010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.248"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(366..966)
                     /locus_tag="ACHJ8C_004616"
                     /pseudo
     CDS             complement(366..966)
                     /locus_tag="ACHJ8C_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012016515.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
     gene            complement(1055..1489)
                     /gene="uspF"
                     /locus_tag="ACHJ8C_004617"
     CDS             complement(1055..1489)
                     /gene="uspF"
                     /locus_tag="ACHJ8C_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO5554322.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(1631..2764)
                     /gene="ompN"
                     /locus_tag="ACHJ8C_004618"
     CDS             complement(1631..2764)
                     /gene="ompN"
                     /locus_tag="ACHJ8C_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO5554323.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSTNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT          814 a          571 c          622 g          766 t
ORIGIN      
        1 atgattttat actgaactaa aggaggaaag aggtaacgaa aaaagacctc caccaggtac
       61 gaatctgagg cgggcagatg gaggtcgata tatacccgat taaatggata agtaaaagcc
      121 ccatcagggg ttgatttgtt cgagtctaat ttaaagtaat ttcaacgatt tggcagaatt
      181 tcttagagtt aatgattttc aagttttcga ttaccaggaa aaaggctggg tgctgataaa
      241 atagtcatct gcattcctta aattgtgtaa gggtgaaagg ataaagttca atttaaaggc
      301 ggtacaatat tagcaccatc ttatatatgt aatgattgat ttgcctcttt attctactgt
      361 tgagattatt gtttccgaaa attgattatt atcagcgcga aaagaaatgc tacagcaaat
      421 aagaggacga ttgtcgacgc tggggctctg tcgagaaaat ataataacca tacgtctgat
      481 attgttattg atatcgttac ggcgataagt aatgccttat gaaagcgatg cgtggtgagg
      541 acagcaatag accctggagc tattaatagt atatagacag aataatttcg actgctttta
      601 atgtggcaaa aatggtaggc gataccatgc agagtaaacc gtaatgtaat aatgatatct
      661 gtaatccgct gacttgtgcc tgttgatagt caagacaaaa attcctgaaa aaacgatacc
      721 tataatggta tctttcttta tgatgctatt atctttcaga tagccagttg caattgcaca
      781 agacagtcct acaataaatg caccaattcc cagcggcata cctataatcc aggctagaac
      841 tatgccatga aacattgcgt ggttcattgc atctcctatt agcgcccagc cttttaatac
      901 cagaaaaact gataataatg cacaggggaa cgatattata atagcgatta tcaatgcata
      961 attcataaat ttaagttgta atgattagag gggaaaatgt cagcacattt gcgaaatgaa
     1021 tcaaaaagcc cgcagcaatg tgcgggcgtt agtgtcagcg cacaaccagc acggagcact
     1081 ctgcgtgacg cactacagct gcggcgttag aaccgagcag ataagtggtg atatccggtc
     1141 gatgggaagc aatgatgatc atatgagcgg ggatcttctt cgccaattcc agaatgcggt
     1201 ctttgggcga gccttcttca acatggacat gcactctgtc ggttggcagt ttaaattttt
     1261 taatgatctc ttccagttgc gatttggctt ccgctttcag gtcatccatt gccggtaatt
     1321 ctgcggaata cgctaaaccc agagaggcat agtagggcag tgaaggtatt accgtcagga
     1381 aatgaacctc tgcatcatca atctttgcct ctgcctcaac gtggctaatc acgcgttgag
     1441 ttaattctga atcggaaata tcgataggga caagaatcgt tctgttcata aaacctcctg
     1501 ttttagtatc cgcataaagt gtaacgccag atgacacttt ttgtgtaatg acggagttca
     1561 catttttaat ttagatcaaa ggaggaagaa taagcagaaa aagcccgcca aaacagcggg
     1621 caggggggat ttagaactga taaaccagac ctaaagcgac gatatcatcg gtagagatgc
     1681 cgttggcagc gtagaaactg tcatcttcat ccaacaggtt gattttatag tcaacatagg
     1741 tggacatgtt tttattgaaa tagtaagtcg cgccaacatc ggcgtattta accagatctt
     1801 tatcatcaac acctgccggg ttgtctgcac caccggcagc gtgcaggtca cggcctttag
     1861 acatcaggaa agagactgcc ggacgcagac caaaatcaaa ctggtactgt gcagtgactt
     1921 caaagttctg ggttttgttt gcaacagcat aatcgctgtc gccaaacggg gtcatattac
     1981 gcgtttctga atacatggtt gccaggtaaa tattgttagc atcgtatttt agcccagcag
     2041 tccacgcgtc tgctttatca ccacccgccg cagtatggtt aacctggtca ttggtgcggt
     2101 cagaagaggt gtatgccgca ccagcgctaa agcccatgcc taaatcatat gttgtggaaa
     2161 gaccccagcc gtcaccgttt tcatggcgaa catcacgtcc gttgttggtg ccttcctgac
     2221 cattactggc accttcgttg ttaccttgat actgcaccgc gaagttcaga ccatttacca
     2281 gaccgaagaa atcagtatta cgataagtcg cgacgccatt ggcacgacca gtcataaagt
     2341 tgtctgcatt ggtataagag tcaccgccaa attcaggtag catatcagtc cagccttcga
     2401 tgtcgtacat tacgccataa ttacgtccgt aatcgaaaga accgtaatct gcaaatttca
     2461 gtccggcaaa tgccagacgg gtccatgact ggtttgttga agattcggtg ttgtttgcct
     2521 gaatattgta ttcccattga ccgtagccag tgagttgatc gttaatttgg gtttcgcctt
     2581 taaaacccag acgagcatag ctctggtcgc catctttcgc tgaattatca gaaaaataat
     2641 gcaggccatc aactttgcca tacagatcta atttgttgcc gtctttatta taaacttcgg
     2701 ctgcatgtgc agcacctgca gcgagcaggg caggaattaa gagtgccagt actttgcttt
     2761 tcattgaata aat
//
LOCUS       DAZFJL010000247         2655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFJL010000247 DAZFJL010000000
VERSION     DAZFJL010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.249"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            170..1096
                     /locus_tag="ACHJ8C_004619"
                     /pseudo
     CDS             170..1096
                     /locus_tag="ACHJ8C_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000011509.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
     gene            1110..2089
                     /locus_tag="ACHJ8C_004620"
                     /pseudo
     CDS             1110..2089
                     /locus_tag="ACHJ8C_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390554.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          718 a          564 c          784 g          589 t
ORIGIN      
        1 accagattag catgacggtg cattcttcaa ggcatatatg gttaatagta tcttcagtag
       61 catgcataag tattgagatg ttttatgcca gctacgaata tattgcatat ctcattctct
      121 gtaagatttc ttattagcac ttagccattt ctagttaaaa ggattaatta tgagttttgc
      181 agctactgat tatccatccg gcgcactagc tggatcgggt acacataatg tgactttgag
      241 cggcagcgca gttcctgatg gaatgttact gtttgcaagc ttagggggga cagaaacagt
      301 aggggaacta tttacttatt ctattaaatt aaaaactcct gacatcctga acctggacta
      361 tgtttccccc gcagccaacc tgcagctcaa accgatggtg ggcaaagatc tgtgcgtcaa
      421 tatcgaactg gatggcggcg gaaaacggta tatcagcggg ctggtgacgg cggcacgcgt
      481 ggccggtcat caaggacgct cggtggtgta tgaactgcga ctcgaaccgt ggttaaaaac
      541 actgacccac actagtgact acaaagcgtt tcagaataaa aacgtggtgg acatcctgga
      601 tgaggtactg gatgagtacc cctggccagt ggaaaaacga ttggtagaaa actatccgac
      661 tcgcgcctgg caggtgcagt acggtgaaac agatttcgat tttattcagc gcctgatgca
      721 ggagtggggt atctactggt ggtttgagca caacgagaac agtcacacgt tagtgctggc
      781 tgatgccatt aatgtccaca aggcatgcgc agattcgccg ctggtgtgtt attaccagaa
      841 agggctgaaa ctggacaagg agtttatcca taccatcaca gcaaatgaaa gcctgcgcag
      901 cggacagtgg gtgctgaatg atttcgactt tatgaagccg cgttcactgc tgaagagtac
      961 tgtggctaat ccgcgtgaaa ccggccttgc tgaatatgag cactacgaat ggccgggaga
     1021 ctacttcaca aagagtgaag gtgaaatgct gacacgcatc cgaatggaag aacagcgtag
     1081 ccccggtagt cgggtacagg gcagcggcca ggcagcttta cgaccccggc gggcgactga
     1141 agcgcaggga gacatacagc gggatgcgcg gggtggttcc ggacacgttc acggaccggc
     1201 agtacagcta cagcggcgag gatgaactgc tgaagacgcg gcacagccgg cggggggaaa
     1261 cggactactt ctatgacacc acggggcgca tcacggcgtg tcgctcggag gatgaggggt
     1321 atctggcgag ctgggagtac gacgcggcgg gcaacctgct ggacaggcgc gcggggggaa
     1381 gggccacggc agaaaacagc gtggtgctgt tcaaccggct gatgtcttac cgtggtgtgc
     1441 actaccgtta tgacgaattt ggccgtgcgg tggaaaagga ggggcggagc ggaacacaga
     1501 gttaccgtta tgatgcggaa caccggatgg tggaagtgac gacagcgcgg gagacatacc
     1561 ggtatgtgta cgatgcgctg gggaggcgga cggaaaacag cacatcagcc ctgacggtaa
     1621 accgtacaac cggacaaaat ttttatggga cggaatgcgg ctggcgcagg agaacaggcc
     1681 ggagggaata agcagcctgt atatctacag cgacccggga agttatgaac cgctggcgcg
     1741 gttggataag gcggggaaag agggaccgaa ccggatactg tattttcaca cggatgtgaa
     1801 cggtgctccg gaagagatga cggacagcaa cgggaaaatt gtgtgggaga cggggtatca
     1861 ggtctggggt aacacgatac aggagaagga ccacggcggg gtggagcaga acctgcgata
     1921 ccaggggcag tacctcgaca gggaaacggg gctgcactac aatctgcaca ggtactatga
     1981 tccggatgtc gggcggttca tggtaacggg cccgataggg ctgcggggcg ggttaaactt
     2041 atatgcttat gcgccgaatc cgttaacgta tattgatcca cttggtcttt gtaaattaag
     2101 gggaagtaat gggcgcttta gaagtgcaca gaatattcag gaagaaatag gcggcctgcc
     2161 caatttttca ggaaaaaccc ccagggcaaa tacggcaaac tttgcgtaat cgagtttatt
     2221 cttcggtaca agcgcatagt ggaggagagg tctggacaaa agcattacct gacggaaata
     2281 ctgttgcagt gagaattgac ccagcaaatc ctcgctcacc ttcacattat gccgatgctg
     2341 tacctcatgc tcatattgaa acagttccaa catccagtgt ttctaatggg aattatcgtc
     2401 cgagagacgt gacgggcagg catactgcca ctggtggggg ttctgaccga ggaacgaatc
     2461 cgaattataa cgctgatgtt cacattccaa tgtctccggg acgatcccca tataattaat
     2521 gtgaggtttt atgaatatcc gaaatcaata taatgaagcc ttaaataaat tagatgttga
     2581 tgttaatgat ggtttgagag atttaataaa catatattgt gtggctattg attcttttga
     2641 aaatgatatc gttga
//
LOCUS       DAZFJL010000248         2605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZFJL010000248 DAZFJL010000000
VERSION     DAZFJL010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.250"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            452..595
                     /locus_tag="ACHJ8C_004621"
     CDS             452..595
                     /locus_tag="ACHJ8C_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO5554324.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            608..817
                     /gene="cspE"
                     /locus_tag="ACHJ8C_004622"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             608..817
                     /gene="cspE"
                     /locus_tag="ACHJ8C_004622"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO5554325.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            983..1792
                     /gene="rlmA"
                     /locus_tag="ACHJ8C_004623"
     CDS             983..1792
                     /gene="rlmA"
                     /locus_tag="ACHJ8C_004623"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO5554326.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVGQLRERLDEKAAAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELVRVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLKGFTLQQSDELCY
                     LMRLRGDEAVALLQMTPFAWRAKPEVWQALAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1789..2355)
                     /gene="mntP"
                     /locus_tag="ACHJ8C_004624"
     CDS             complement(1789..2355)
                     /gene="mntP"
                     /locus_tag="ACHJ8C_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO5554327.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          688 a          605 c          620 g          692 t
ORIGIN      
        1 ttattagcga aacgtttctc tcttgatttt tttgctcatt catcaatttt tcttatttta
       61 aatttacaat ccctttgggg attgacttct ctttagggta attgatagcc gttaactgac
      121 tgttttatga gaaaaagtga tataactttt tattcattgc atagcaaaaa atgtgatatt
      181 gcacgcacta tgtaataact tctcccactg gcctggaaca actgaactta ttgaactatg
      241 ttagaaaata cgccagttta agtatctgcc tgaactggca aggttaagca caatgatata
      301 tcggcgcgta ttccgttgca taagtgtgca aaaaaagtgg aagacgtatc gagatttgtg
      361 cgtctgatcg agacatgttt aaaaatggct tgccataatt aacgttgtat gtgataacag
      421 atttcgggtt aaacgaggta cagttctgtt tatgtgtggc attttcagta aagaagtcct
      481 gagtaaacac gttgacgttg aataccgctt ctctgccgag ccttatattg gtgcctcatg
      541 cagtaatgtg tcagttttat ctatgttatg cctgcgggcg aagaaaacaa tctaaggaat
      601 ttttcaaatg gcaaagatta aaggtcaggt taagtggttc aacgagtcta aaggttttgg
      661 cttcattact ccggctgatg gcagcaaaga tgtgttcgta cacttctccg ctatccaggg
      721 taatggtttc aaaactctgg ctgaaggtca gaacgttgag ttcgaaattc aggacggtca
      781 gaaaggtccg gcagctgtta acgtaacagc tatctgatcg aatccactga tctgaagtgt
      841 gaatacgctt caatctcgct ataaagcctc gtcgaatgcg aggcttttta ctttgtttta
      901 tcttcgctcc agtcgttcgg atatttgccc gccgcatgat tcgcgttaca cttgcggcct
      961 ttagtatcct gccggagttg tcatgtcgtt ttcctgtcca ctttgccatc agcctctttc
     1021 gcgtgaaaaa aacagctata tctgtcccca gcgacatcag tttgatatgg cgaaagaagg
     1081 gtatgtcaat ctgctgcccg ttcagcataa acggtctcgt gatccgggcg acagcgcgga
     1141 aatgatgcaa gcacgccgcg cattcttaga tgccggacat tatcagccgc tgcgtgatgc
     1201 aattgtcggc caactgcggg aacggcttga tgaaaaggcc gcggcggtgc tggatattgg
     1261 ctgtggtgaa gggtattaca cacacgcatt tgctgatgcg ttgcccgaaa tcaccacgtt
     1321 tggcctggat gtctcgaagg tagcgataaa agcggcggcg aaacgctatc cgcaggtcac
     1381 tttttgtgtc gcttccagcc accgtttgcc gttttccgat accagtatgg acgccataat
     1441 acgtatttac gcgccgtgta aagcagaaga attagtgcga gtagtgaagc ccggcggttg
     1501 ggtcattact gccacgccgg gaccgagaca cttaatggag ctgaaggggc tgatttacaa
     1561 tgaagtacat cttcatgcac ctcatgcaga acaactgaaa ggttttacat tacagcagag
     1621 tgatgagttg tgttatctga tgcgtcttcg cggtgatgaa gccgtcgcat tattgcagat
     1681 gacgccgttt gcctggcgtg cgaagcctga agtctggcaa gcactggccg caaaagaagt
     1741 gttcgactgc cagacggact ttaatattca cctctggcag cgttcttatt aaccgtggaa
     1801 gtgcgtccag aggatctgga cgccaatacc gatcagtacc agcccgccga gaatttccgc
     1861 ttttttccca ataattgagc cgataaagcg accaaccatc atccctaatg tcgacataat
     1921 caaggttgca caaccaatgg ccaatgcggt cgcgataatg ttaacctgca ggaaagcaag
     1981 accaacaccc acagccatgg catccaggct ggtggcaatc gcggtggtta ccagtagcca
     2041 gaaaccgtgt cgacggcgcg gttcttcatc ttcatcatct gcgccacgaa aaccctcaat
     2101 aatcattcgc ccgccgagaa atatcagcag cacaaacgca atccagtggt tccattcaag
     2161 gacaaaccgg ctggctaaca tgcccattcc ccagccgatc agcggcgtca gggtttcgac
     2221 ggcaccaaaa ataaggccgg ttcgcaatgc ttcagaaaat ttcggtttat ggagggtggc
     2281 acctttaccg attgatgcag caaaggcatc catcgacata ccaaacgcaa gaagaacagt
     2341 agcagtgata ttcatgacaa cgtcctgacc ggggtatcca taaaacacat catcgccccc
     2401 agcaaacatg catacatgaa caacaagccg cgatggtttt cgcgttcatg caggcatagc
     2461 gattgatgtg catatggtct cgcctgactg aaagtgttca gtccgtacgt gccacgtttt
     2521 gcaacgagta tgttgacacg tacatttccg gaatctggaa atcagctact ccccaaatga
     2581 gggcgcaacc ttaacatatt ttgag
//
LOCUS       DAZFJL010000249         2504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZFJL010000249 DAZFJL010000000
VERSION     DAZFJL010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.251"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            738..1157
                     /locus_tag="ACHJ8C_004625"
     CDS             738..1157
                     /locus_tag="ACHJ8C_004625"
                     /inference="COORDINATES: protein motif:HMM:NF017542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HFO5554328.1"
                     /translation="MANTTRKKVKIVGKKQYLDPTTGEVSDFQVINIEERDANFHKIW
                     LSNVIQSLDLIGNQKIRFAFWLMDQANKDNEITLTLRQMSELSKISLETVRLTVKSLI
                     ESDFLRRKNMGVYQINPNSIFKGGNGNRLNVLFSYQK"
     gene            complement(1434..>2504)
                     /locus_tag="ACHJ8C_004626"
     CDS             complement(1434..>2504)
                     /locus_tag="ACHJ8C_004626"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554329.1"
                     /translation="FSNTPHRNLEKTIHSRFGTLLMLLPPLNTPFCDWITVTYRSERP
                     DLSYITRLFFEGLHASVHPLGGVDSGWSAIGNPRMALTGAGSELTGIPITQFVILARF
                     ADKSYHLVFQISGLGLQLLRSEPELFRELMRELGSCPHRVTRLDVGMDLPVDGADYLA
                     DFKSRFPSGYVQFTRKRAALSWVLSVRDDGRDTGTVYAAKGTRSRVKMRIYDKAYEVL
                     QRRKLTIPPRTRVEFALETEFGASLYDALRPESLFWHLASPDILGKKPADVPAWFKRE
                     SFTPAPRERRYKSDKPPLHVMKSYVDDTQLLDGLIEYVDRNIGSDGRMLLKNHIGRYI
                     DQYDESRKILLSDKSTTTNDAS"
BASE COUNT          805 a          555 c          581 g          563 t
ORIGIN      
        1 agaggcaaaa tcaaggacat ccgactcacc atcagggatt agacgggcga cggcggaaaa
       61 accatcaaca gaatcacgga gctggcaaaa agattgataa acatcttcag ggatggaacg
      121 atcgtgaaca gtcatatagg cctccattca agccgtaagg tttatgtgat tagaaaagcc
      181 ccttagagcc acctgagggg cttttcgcta ttagatcacc caaacccaca cctttcaacg
      241 ttttgctcgt cagagagcgt tttagacccc ttattttcga tttttttact acgttgttgt
      301 cgtcagtcgt cacggcaggc gctcccgtcc tcgtccctgc gggcgggtgc ggctggcgtt
      361 cctccttcct ccaaccactc aagacaaaaa ccgataaccg gagatcaaaa cgaccgagag
      421 agaagaaacc gctcgccgca gacccagcga ccgaacgtag tgagcgagtg gtcgaggaag
      481 cggaagagaa ccggacagac atgaagcact tacgcgctgt tataaaacat agataagagt
      541 gagcaaaagc ctgaatcata gataactcat tgttgcctat aacttttgca ggaaaaatcc
      601 tgtactagat ttctaggtgt cttgtactag atttctggca caaaacacca aaaaaaaata
      661 gtacaaaaag cattgactag gacaagagac ctatttacat taggacttgt ggtaggcatt
      721 taatcaagtg gggcaatatg gccaatacaa cgcgtaagaa agtaaaaata gtaggaaaaa
      781 agcaatatct cgatcctaca actggggaag tctctgattt tcaagtgata aatattgaag
      841 agagggacgc taatttccat aagatatggc tgtcaaatgt aattcaaagt ctagatttaa
      901 taggaaacca aaaaattagg ttcgcatttt ggttaatgga ccaagcaaac aaggataacg
      961 aaataacatt aacgttgcgt cagatgtctg agttatcaaa gataagttta gagacggtca
     1021 gactaacagt gaagtcatta atagaaagtg attttttacg tagaaaaaac atgggcgttt
     1081 atcagataaa cccaaactct atatttaaag gaggtaacgg taatcgactt aatgtacttt
     1141 tttcatatca aaaatagagc aaaaacgaga gtgcgtggcg tgaattcttc aggcccggac
     1201 acgccacccc cgccccacgc gacagcgtgt gacgtgtgtg tcgcggccgg gcgaggatga
     1261 atatagcctg agccttcccc gtgtattctt tgtacgacac agttaatata tttaaaagat
     1321 attagtcacg ttttagaaaa cagggttaat aagcaatgca aaaagaaaat atataaacag
     1381 aagatatttc ccgcgtggcg aatgtaatta atacagagca cgcaggaaat aaattaactt
     1441 gcgtcgttag tggttgtcga tttatcagaa agcaatattt tccgactctc atcatactgg
     1501 tcgatataac ggccaatatg atttttcagc aacattcgac cgtcagaacc gatatttcga
     1561 tcaacgtact ctatcagccc atcaagcagc tgcgtgtcgt caacatacga tttcataacg
     1621 tgaagtggtg gtttgtcgct cttatatcga cgttcgcgag gcgcaggtgt aaaggattcg
     1681 cgtttaaacc aggctggaac atcagccggt ttcttaccca gaatatcagg cgatgcaaga
     1741 tgccagaaca aagactcagg acggagggcg tcatagagcg atgcaccaaa ttccgtctcg
     1801 agcgcaaact caactcgcgt acgcggcgga atggtaagtt ttcgccgctg taacacctcg
     1861 taagccttgt catagatacg cattttcaca cgagagcgcg tacctttagc cgcgtatacc
     1921 gtacccgtat cacgaccatc atcacggacc gacaaaaccc agctaagggc ggctctctta
     1981 cgcgtaaact gaacataacc agaagggaaa cgacttttga aatcagcaag ataatcagcc
     2041 ccgtccacgg gcaaatccat gccaacgtcg agacgcgtaa cacggtgagg acaagaaccc
     2101 aactcacgca ttaattcccg gaacagttca ggctctgagc gtagcaactg aagaccaagc
     2161 ccggagatct gaaaaacgag gtgataactc ttgtcagcga accgcgcgag gataacgaac
     2221 tgagtgatcg gaatacctgt aagttcagaa cccgcacctg taagagccat gcggggattg
     2281 cctatagcag accaaccaga atcaacacca ccaagcggat ggaccgaggc atgtaaacct
     2341 tcaaagaaca aacgggtgat gtacgacagg tcaggacgct cagaacgata cgtgacagtg
     2401 atccagtcac agaaaggagt gttgagagga ggaagcagca taagcagagt accaaagcga
     2461 ctgtgaattg ttttttccaa atttctatgg ggggtgttac taaa
//
LOCUS       DAZFJL010000250         2488 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFJL010000250 DAZFJL010000000
VERSION     DAZFJL010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.252"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            699..1154
                     /locus_tag="ACHJ8C_004627"
     CDS             699..1154
                     /locus_tag="ACHJ8C_004627"
                     /inference="COORDINATES: protein motif:HMM:NF017542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HFO5554330.1"
                     /translation="MTKMPPKKIKDKNGNAHYVGRKMLTDLETGEQFEAQTIIKSVGD
                     KDFKKLFIGAILDKIDAFSSAKLKFILWLIENADKQNRIIGTFKQLSDESGVSFATVA
                     RLMPMLREADILRLKSPSVYIINPDLVTSVSSNSRQALLVRYKEIESKH"
     gene            complement(1417..>2488)
                     /locus_tag="ACHJ8C_004628"
     CDS             complement(1417..>2488)
                     /locus_tag="ACHJ8C_004628"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554331.1"
                     /translation="FSNTPHRNLEKTIHSRFGTLLMLLPPLNTPFCDWITVTYRSERP
                     DLSYITRLFFEGLHASVHPLGGVDSGWSAIGNRRMALTGAGSELTGIPITQFVILARF
                     ADKSYHLVFQISGLGLQLLRSEPELFRELMRELGSCPHRVTRLDVGMDLPVDGADYLA
                     DFKSRFPSGYVQFTRKRAALSWVLSVRDDGRDTGTVYAAKGTRSRVKMRIYDKAYEVL
                     QRRKLTIPPRTRVEFALETEFGASLYDVLRPESLFWHLASPDILGKKPADVPAWFKRE
                     SFTPAPRERRYKSDNPPLHVMKSYVDDTQLLDGLIEYVDRNIGSDGRMLLKNHIGRYI
                     DQYDESRKTLLSDKASTTDKAS"
BASE COUNT          819 a          550 c          575 g          544 t
ORIGIN      
        1 agaacaaaaa tcatcctcac catcagggat taatttggcg acggcggaaa aaccatcaac
       61 agaatcacgg agccggcaaa aagattgata aacatcttca gggatggaac gatcgtgaac
      121 agtcatatag gcctccattc aagccgtaag gtttatgtga ttagaaaagc cccttagagc
      181 cgcctgaggg gcttttcgct attagatcac ccaaacccac acctttcaac gttttgctcg
      241 tcagagagcg ttttagaccc cttattttcg atttttttac tacgttgttg tcgtcagtcg
      301 tcacggcagg cgctcccgtc ctcgtccctg cgggcgggtg cggctggcgt tcctccctcc
      361 tccaatcact caggacaaaa accgataacc ggagatcaaa acgaccgaga aaggagaaac
      421 cgctcgccgc agacccagcg accgaacgta gtgagcgagt ggtcgaggaa gcggaagaga
      481 accgaacaga catgaggcac ttacgcacgg tataaaaaca atagataaag agcgaaattt
      541 gcaaagtgct tgtaaaacaa aaagttacag aaatactcat tatcaaagtt gagaatctcc
      601 gttatcaaaa ttgagtatct gcgttatcaa aaaaagataa tgacaagcgt gtattatcac
      661 cttaagataa cgcaacttag cataaaggag agaatggaat gacgaaaatg ccaccaaaga
      721 aaataaaaga caaaaatgga aacgctcact atgtagggcg aaaaatgctt acagacttag
      781 aaacaggaga gcaattcgaa gcccaaacga taataaagtc ggtaggagac aaagacttta
      841 aaaaactatt tattggagca attctagaca aaatagacgc atttagctca gcaaaactga
      901 aatttatatt atggttgatt gagaatgcag ataaacaaaa tagaattata gggacgttta
      961 aacaattatc tgatgagtct ggagtgtcat tcgcaacagt ggcaaggctt atgccaatgc
     1021 taagagaagc agatatctta agattaaaat caccatcggt atatattatt aatcctgact
     1081 tggtgacatc tgtatcatca aacagcagac aagcattatt agtaagatat aaagaaatag
     1141 aatcaaagca ttgacgggag agcgtggcgt gaattcttca ggcccggaca cgccaccccc
     1201 gccccacgcg acagcgtgtg acgtgtgtgt cgcggccggg cgaggatgaa tatagcctga
     1261 gccttccccg tgtatttttg tatggaacag ttcatatatt aaaaagatat tagctgcgtt
     1321 ttagaaagca gggttaataa gcaacgaaaa aaaatataaa cagaagttat tacccgcgtg
     1381 gcaaatgtaa ttaataaaga gcacgcagga aataaattaa cttgctttgt cagtggttga
     1441 tgctttatca gaaagcaatg ttttccgact ctcatcatac tggtcgatat aacgaccaat
     1501 atgatttttc agcaacattc gaccgtcaga accgatattt cgatcaacgt actctatcag
     1561 tccatcaagc agctgcgtgt cgtcaacata cgatttcata acgtgaagtg gggggttgtc
     1621 gctcttatat cgacgttcgc ggggtgcggg tgtaaaggat tcgcgtttaa accaggccgg
     1681 aacatcagcc ggtttcttgc ccagaatgtc aggggatgca agatgccaga acaaagactc
     1741 agggcgaaga acatcataga gtgacgcgcc aaactcagtc tcgagcgcga actcaactcg
     1801 cgtacgcggc ggaatggtaa gttttcgccg ctgtaacacc tcgtaagcct tgtcatagat
     1861 acgcattttc acacgagagc gcgtaccttt agccgcgtat accgtacccg tatcacgacc
     1921 atcatcacgg accgacaaca cccagctaag ggcggctctc ttacgcgtaa actgaacata
     1981 accagaaggg aaacgacttt tgaaatcagc aagataatca gccccgtcca cgggcaaatc
     2041 catgccaaca tcaagacgcg taacacggtg aggacaagaa cccaactcac gcattaattc
     2101 ccggaacagt tccggctctg agcgtagcaa ttgaagacca agcccggaga tctgaaaaac
     2161 gaggtgataa ctcttgtcag cgaaccgcgc gagaataacg aactgagtga tcggaatacc
     2221 tgtaagttca gaacccgcac ctgtaagagc catgcggcga ttgcctatag cagaccaacc
     2281 agaatcaaca ccaccaagcg gatggaccga ggcatgtaaa ccttcaaaga acaaacgggt
     2341 gatgtacgac aggtcaggac gctcagaacg atacgtgaca gtgatccagt cacagaaagg
     2401 agtgttgaga gggggaagca gcataagcag agtaccaaag cgactgtgaa ttgttttttc
     2461 caaatttcta tggggggtgt tactaaaa
//
LOCUS       DAZFJL010000251         2469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFJL010000251 DAZFJL010000000
VERSION     DAZFJL010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.253"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            89..598
                     /gene="eco"
                     /locus_tag="ACHJ8C_004629"
     CDS             89..598
                     /gene="eco"
                     /locus_tag="ACHJ8C_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO5554332.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(748..2394)
                     /gene="mqo"
                     /locus_tag="ACHJ8C_004630"
     CDS             complement(748..2394)
                     /gene="mqo"
                     /locus_tag="ACHJ8C_004630"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO5554333.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNSWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAVDSTPKPQLKPQLVQKEVADIAL"
BASE COUNT          592 a          669 c          612 g          596 t
ORIGIN      
        1 tgaatgttct ttaagttatt cataaagcaa attaataaat ctgatgaata tgttaacctt
       61 cagcgacatc atcggtgaaa acctataaat gaagaaggaa agcaaaaaaa tgaagaccat
      121 tctacctgca gtattgtttg ccgctttcgc caccacttcc gcctgggcgg cagaaagcgt
      181 ccagccactg gaaaaaatcg caccttatcc acaagctgaa aaaggcatga agcgtcaggt
      241 gattcagtta actccgcaag aagatgaatc taccctgaaa gtagaactgt taatcggtca
      301 gacgctggaa gtcgactgca atttgcatcg tctcggcggg aagctggaaa gcaaaacgct
      361 ggaaggctgg ggctatgatt attatgtctt tgataaagtg agctcgccgg tttccacgat
      421 gatggcctgc ccggatggca agaaagagaa gaaatttgtc accgcgtatc tgggcgatgc
      481 tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg tatacgccag acaatgtgga
      541 tgtgaagtac cgcgtctgga aggcggaaga gaaaattgac aacgcggttg ttcgctaaac
      601 tgctgtgaag tgctgcaacc tgtaggccga ataaggcggt tacgctgcat ccgacatcta
      661 acgcccgagc cggttgcctg atgcgacgct gaagcgtctt atcagcatac gccacatccg
      721 gcaaaccatg ccggatgtgg cacatcatta caacgcaata tccgccactt ctttttgaac
      781 gagttgcggt ttcaactgcg gtttcggcgt actatctact gcctgcggct tgtcgtattt
      841 cagccccagc acttcgctgg tgtactgcaa ctcgcgttct gttgccgcta catcaccgtt
      901 cagcttgcgt ccataagacg gaacgatcgc tttcaacgta gcctgccatt gcgggctgga
      961 aacgcgatcg ccaaatactt tttccagcag attcaacata atcggcgcgg cggttgacgc
     1021 ccctggcgat gcccccagga gcgcggcaat ggtcccttgc tggtcactga cgacttcagt
     1081 acccagacgc agtacgccac ctttatcggc atcacgcttg ataatctgca cgcgctgccc
     1141 cgcttgccac aaacgccagt cctctttttt cgcctgcgga tagtactctt tcaacgcttc
     1201 aaaacgatcc tcttcactca acatcacctg actcaccaga tatttcacca gatcgaaatt
     1261 atccagccca acgtgcatca tcggcatcac gttagaggtg gtggtggaac tcatcagatc
     1321 ccacaatgaa ccgtttttga ggaatttggt tgagaaggtg gcaaatggcc caaacagcac
     1381 tacgcgttta ccgtcaagaa cacgggtatc gatatgcgga accgacatcg gtggtgcgcc
     1441 aacggatgct ttaccgtaaa ccttcgccag atggtgatta accacgtccg ggttttccga
     1501 aacaaggaac tgtccgccca ccgggaaacc ggcgtagtct ttcgcctccg gaatccccga
     1561 ttcctgtaac agtttcagcg ccgcaccgcc cgcgccgata aagacaaatt tggcgcggat
     1621 gttctgtgca gtgccgtttt tcagatcggc aacggtgacg gtccagctgt tatcgtcatt
     1681 acgctttagg gcgcggactt cgctgctgag ttgcagcgag aagttagatt tcttctgtaa
     1741 ggaggcgatc aactggcggg tgatctcgcc atagttcaca tcagtgccaa tttctgtgcg
     1801 tgttgccgct actttctgtt gcggatcgcg cccttccatc actaacggtg cccactcttt
     1861 gatctgcgcg tgatcttcag agtaacgcat accgcgaaac agcgagcttt gttgcaacgc
     1921 ggcgtaacgg gcgcgcagga aattaacgtt atcctcgccc cagacaaagc tcatatgcgg
     1981 aacggtattg ataaatgaac gcggagtacg cagcacaccg cgctcgacct ggtgcgccca
     2041 gaactggcgg gaaatctgaa atgcttcgtt aatggcgact gctttttcaa tactgatgct
     2101 gccgtcggca ttttgcgggg tgtaattgag ttccatcagt gcagaatgcc cggttccggc
     2161 gttgttccag ccgttcgaac tctcctgcgc gacaccttcc aggcgctcca ccatggtcat
     2221 cgaccattca ggctccagct cgcgtaaata ggtccccaac gtggcgctca taatgccgcc
     2281 gccaatcaac agtacatcag tttcctgctc ctcggacgct ttcgcttttg ccgccatcga
     2341 aacggcatta agccccacgg ccatcgagaa gagcatggca gtcacttttt tcatcttgtt
     2401 aatgccttac ttttagtcgc tttattgcag gtgagatttg cgcggcatca acggtaacac
     2461 ttaagtaac
//
LOCUS       DAZFJL010000252         2431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZFJL010000252 DAZFJL010000000
VERSION     DAZFJL010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.254"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(196..1608)
                     /gene="pykF"
                     /locus_tag="ACHJ8C_004631"
     CDS             complement(196..1608)
                     /gene="pykF"
                     /locus_tag="ACHJ8C_004631"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO5554334.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1616..1810)
                     /locus_tag="ACHJ8C_004632"
     CDS             complement(1616..1810)
                     /locus_tag="ACHJ8C_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554335.1"
                     /translation="MDCRTQRLKNRHSRWAKTQAHIPLHAFSMSPILRARHNHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            2165..2374
                     /gene="fumD"
                     /locus_tag="ACHJ8C_004633"
     CDS             2165..2374
                     /gene="fumD"
                     /locus_tag="ACHJ8C_004633"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO5554336.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          643 a          520 c          598 g          670 t
ORIGIN      
        1 caaagaagca tcgaacgctg ggttttagat aaaaaaattg agagaaaaca ggatgcttcc
       61 atcggattca tcttagataa agtgagatta tatagtgaaa tccgatttga ttttttattg
      121 cttctggtta tcgattaaat aaaaaaagcg cccatcaggg cgcttcgata tacaaattaa
      181 ttcacaaaag caatattaca ggacgtgaac agatgcggtg ttagtagtgc cgctcggtac
      241 cagtgcacca gaaaccataa ctacgacatc acctttgtgt gccagaccgc tctgcagagc
      301 cagttcttta cccagacggt agaagtcatc agtagaagtg atctctttaa ccagctgcgg
      361 cacaacacct ttgctcagta ctaactggtg agccgttttt tcgttggtgg tcagtgccag
      421 tatggtggca tccgggaagt atttacgtac tgcgcgagca gatttaccgc cctgggtagc
      481 aaccacgatc agcggggcat ccagtttttc agcagtttca acggcaccac ggcataccgc
      541 ttcggtaatg cgcagtttac ggttgtcatt gttgaactcg agacggctgt tcatcacgcg
      601 gtcggtacgt tcgcagatgg tcgccatgat agaaaccgct tccagcgggt atttaccttt
      661 tgcggattca ccagacagca tcactgcgtc agtaccgtcg aggatggcgt ttgcaacgtc
      721 accggcttct gcgcgagtcg ggcgtggatt tttgatcatg gaatccagca tctgggtcgc
      781 agtgataaca actttacgtg cacggataca tttttcgatc atcatcttct gggcgaagat
      841 aacttcttct accgggattt ctacacccag gtcgccacgc gcaaccatga tgccgtcaga
      901 ggcttcgagg atttcgtcga agttgttgag gccttcctgg ttttcgattt tggagatgat
      961 gtggatgttt tcgccgccgt gcgctttcag gtgctcacgg atttcgataa cgtcagaacg
     1021 cttacgaata aaggaagccg caacaaagtc tacgccttgt tcgcaaccaa agatcaggtc
     1081 ctgtttgtct ttttcagcca gtgctggcag ggcaatggaa acgccaggca ggttcacacc
     1141 tttgttttcg cccaggtcgc cgttgttcag cactttacag ataactttgt taccttcaat
     1201 ggcggtaact tccataccga tcagaccgtc gtcaaccagt acggtgttgc caacagacag
     1261 gtcagtagtg aaaccttcat acgttaccgc aaccatttcg ctgttgccga taacagattt
     1321 atcagtagtg aaagtaaagg tctgacccgc tttcagagaa acgtcgttac cgccttccag
     1381 tttcatggtg cggatttctg gacctttggt atcaagcagg atagcggcgg ttttaccagt
     1441 tttgctcatc acgttgcgca gattctgaat gcgctgaccg tgttctgcat agtcaccatg
     1501 agagaagttc agacgcataa cgttcatgcc agcgtccagc attttagcta acatctcttc
     1561 agattcggtt ttcggtccga tggtgcaaac aattttggtc tttttcatga cagtcttagt
     1621 ctttaagttg agaaggatgg gagaaacttg ctttctgggc gcactgccgg aaagcgaatc
     1681 cggtattacg aaagtggttg tgcctcgctc taaggatagg tgacatcgaa aaagcgtgca
     1741 gaggaatgtg tgcttgtgtc ttagcccaac gtgagtgcct gtttttgagt cgttgagttc
     1801 tacagtccaa agtgctgaaa ccattcaaga gtcaattggc gcgcattata cgctgaaatt
     1861 aatccaaaag gaaaacaaaa acagcgtttc aaaaaatata caacttgtaa gacaataaat
     1921 tgttatcaaa gattcagcgt tccagggaaa aggttacgca aaaacttcgt tgtttgcgca
     1981 acgtctggtg ccggggttgt cagtgtgagg ataagtgaaa gttcatccgg tgtataaata
     2041 catcatcgct caattgctac agttaaaata atttatcacg ttatttgtct gattttgttt
     2101 gatttttgtt taattggtta gattgatata tgctgatagt tggcatgata aataggtata
     2161 aagaatggga aaccggacaa aagaggatga actgtaccga gaaatgtgca gagttgtcgg
     2221 taaagttgtg ctggaaatgc gcgatctggg gcaggaacca aagcatattg ttatcgcagg
     2281 cgtattacgt acagcattag ccaataaacg cattcagcgc agtgagctgg aaaagcaggc
     2341 gatggaaaca gtcattaacg cactggtaaa atgaccattc aggatgaggc ggtaagcttc
     2401 atcctgatat tgttagttca gttgcggtcc a
//
LOCUS       DAZFJL010000253         2390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFJL010000253 DAZFJL010000000
VERSION     DAZFJL010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.255"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(1..164)
                     /locus_tag="ACHJ8C_004634"
                     /pseudo
     CDS             complement(1..164)
                     /locus_tag="ACHJ8C_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012539895.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(526..1022)
                     /locus_tag="ACHJ8C_004635"
                     /pseudo
     CDS             complement(526..1022)
                     /locus_tag="ACHJ8C_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820650.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
     gene            complement(1158..1346)
                     /locus_tag="ACHJ8C_004636"
     CDS             complement(1158..1346)
                     /locus_tag="ACHJ8C_004636"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554337.1"
                     /translation="MIAVVPSRMVPNNSDLTIIPLPIKIPGFTKSMAWHERTHHDPAH
                     QWIRALCFEINQHSTTVI"
     gene            1559..2261
                     /locus_tag="ACHJ8C_004637"
                     /pseudo
     CDS             1559..2261
                     /locus_tag="ACHJ8C_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          675 a          520 c          517 g          678 t
ORIGIN      
        1 ttaaatattt ttgtgcaata tgtccggtta aagtgctcaa tgagcgcgtt cagctttggc
       61 ttacccggct ggataaccta cggttttaat gcatatttct ctgcctgttc agcctgtgtc
      121 agcaagataa attttggatt attgtccatg cgaagcatag ttggaatagc gctctccttc
      181 gttaacaaac tgatgatgtt ggacaccctc taatgaaagt ggcacttccc ccaacttagg
      241 ggagattttc atgccagtgg actcatttcc cttcgcctca gtaattcgaa tccagaacaa
      301 ccatacatct gtagtttcat cattttcaga cgatttacat ggtcctcggc aataccatta
      361 cttcagctac tgataagaat ttcggtgatt ttttaggtga ccgttcactg gttgcgaaaa
      421 cgccactttt tacccgtggc taatagtaag agtaaaaaag tgataaaaat ttttgaggtt
      481 gcgattaagc gcaaccttag tgatatgaca gtagaaagtc agtcattatt tcgttgctgc
      541 ttcaactagg ccatcaagcc atgcctggtg accattcagc attgggttcg ggcgagtatt
      601 tgccagttct tttgccggaa ctccgttctg tgtttcctgc gtcaggatac gtacccggtt
      661 actggaaaga tcctccagta accatgcatg atgtacatcg agacgttgag tagtattttc
      721 ttcaccggac catccgtgcc aggccagacg agcaggtttg ccttccatcg gtgggacaaa
      781 ctccacaacc tgagcttcaa caggaaaacc aaaggttttg aaatagaaac gcaccccatt
      841 ttccagttct ggctttttgt tatcgtaaaa gcgaatatca gcacaatttt tggccacagg
      901 aatggctgac tcagaagcgg ccagatatct ttaacattaa gaccagagat aatcatttca
      961 ttggaaacaa agttatcagt aaaaccagga acaaaacctt caggccagtt aatctcgttc
     1021 ataaatttct ccagttacgg taattaattg tgctgagcat gttgcttcag aagccgtatt
     1081 caatgtggca gcaacaatta ttgagattat tgtgttataa tgccaatcgt tattctttat
     1141 gatctgaata agaaaactta tatcaccgtt gtggagtgct ggtttatctc aaagcacaaa
     1201 gcgcgaatcc attgatgggc tggatcatga tgagtacgct cgtgccacgc catgctttta
     1261 gtgaagcctg gaattttaat cggtaaggga atgatagtaa ggtcactgtt gttcgggacc
     1321 atacggctgg gtacaacagc aatcatgtca gttaatcgta atatgtctgg tatcaccaaa
     1381 aagctgttaa cagaataccg acacgtcgct tgagatttaa ctcagccagt acctcatccg
     1441 ttacaccagt aaaatgacct tctgtggaca ccagaatatg ttcctgttca cagaattgtt
     1501 tgatcgtcat ttttgaattg tgtgattcat agacatcgtt tcactataat acctcactat
     1561 gtacagggaa attcagcagc ggctacagtg ggtaaaattc tatgaaacgt ccggtgatgc
     1621 gggatttata tgccgtcgcc tcaagcgttc accatcaacg aaaaccggta cttgcgaagt
     1681 cgctctgatt ctggagttac gttctcagcg aaatctgggt gcaagacgta ttcaaagcga
     1741 gttaaaacga ctcactggca atagcaacaa tccacaaagt cctttgccag aatcaggtta
     1801 aacctgttgt taaatttcgg cgtgaagttg atttcattcg ctacgaacgc cctgttcccg
     1861 gtgatcgggt tcagatggat acctgtaaaa ttggccccgg cttatatcag tacacatctg
     1921 ttgatgattg cactcgctac agagtcctga ggctctactc tcgtcgcacg gcggtaaact
     1981 cactggattt tattgattgt gttattgaag aaatgccttt cccgatatag cgctctcaga
     2041 cagaccggag aagagaattt tttgccgtta aagtgcagga aagattaaaa taatacggca
     2101 tcaagtttcg tccaaataag ccagcgtcac ctcatcttaa cggaaaggtt gaacgttccc
     2161 aaaagacaga caaagcagag tcttacgcta ccgtagatct ttctgctgat gacctgaagg
     2221 aactgcaggc agaatggcag cattactata actgggaacg tctgtagtgg cacactgaat
     2281 ttggccacct gaacagaggt gatatgctca cctcagaaca acacaggtgc tccaatgaaa
     2341 aaaagaaatt tcagcgcaga gtttaaacgc gaatccgctc aactggttgt
//
LOCUS       DAZFJL010000254         2386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZFJL010000254 DAZFJL010000000
VERSION     DAZFJL010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.256"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..1176
                     /gene="escC"
                     /locus_tag="ACHJ8C_004638"
     CDS             <1..1176
                     /gene="escC"
                     /locus_tag="ACHJ8C_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFO5554338.1"
                     /translation="LKYLSDTISVNKNSCNVRKITTFNSIEVRGVPECIKYITSLSES
                     LDKEAQSKAKNKDVVKVFKLNYASATDITYKYRDQNVVVPGVVSILKTMASNGSLPST
                     GKGAVERSGNLFDNSVTISADPRLNAVVVKDREITMDIYQQLISELDIEQRQIEISVS
                     IIDVDANDLQQLGVNWSGTLNAGQGTIAFNSSTAQANISSSVISNASNFMIRVNALQQ
                     NSKAKILSQPSIITLNNMQAILDKNVTFYTKVSGEKVASLESITSGTLLRVTPRILDD
                     SSNSLTGKRRERVRLLLDIQDGNQSTNQSNAQDASSTLPEVQNSEMTTEATLSAGESL
                     LLGGFIQDKESSSKDGIPLLSDIPVIGSLFSSTVKQKHSVVRLFLIKATPIKSASSE"
     gene            1190..1645
                     /gene="cesD"
                     /locus_tag="ACHJ8C_004639"
     CDS             1190..1645
                     /gene="cesD"
                     /locus_tag="ACHJ8C_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFO5554339.1"
                     /translation="MSRKFSSLEDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNKKMAKKAFKACLMWCNEKEKYTTYKENIKKLLGNTE"
     gene            complement(2029..>2386)
                     /gene="grlA"
                     /locus_tag="ACHJ8C_004640"
     CDS             complement(2029..>2386)
                     /gene="grlA"
                     /locus_tag="ACHJ8C_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE transcriptional
                     regulator GrlA"
                     /protein_id="HFO5554340.1"
                     /translation="DGEPLYILVSLWCKLQEKWISRNDIAEAFGINLRRASFIITYIS
                     RRKEKISFRVRYVSYGNLHYKRLEIFIYDVNLEAVPIESPGSTGPKRKTYRVGNGIVG
                     QSNIWNEMILRRKKES"
BASE COUNT          791 a          465 c          432 g          698 t
ORIGIN      
        1 ctaaaatatc tgagtgatac aatctcagta aacaaaaact cttgcaatgt gagaaaaatt
       61 acgacattta actcgattga agttcggggt gttcctgaat gtataaaata tattacatct
      121 ttatcagaaa gtcttgataa agaggcacag tctaaagcca aaaataaaga tgttgttaag
      181 gtctttaaac tgaactatgc gtcagcgaca gatataacat acaaataccg tgaccaaaat
      241 gttgtcgtcc caggtgttgt ctccattctg aagacaatgg caagtaatgg ttcgcttccc
      301 agtaccggta aaggtgccgt cgagcgaagc ggtaacctgt ttgataacag tgtaacgatt
      361 tccgctgatc cacgtcttaa tgcagttgtt gtaaaagacc gcgaaataac gatggatatc
      421 tatcagcaat taatttctga gcttgatatt gaacaaaggc aaattgaaat ttctgtctca
      481 attattgatg ttgatgcaaa cgatcttcag cagctcggcg taaactggtc cggtacgtta
      541 aatgcaggac aaggtacgat tgcctttaat agctctaccg cacaggctaa tatcagctct
      601 tctgtgataa gtaatgccag taattttatg attagggtga atgcgttaca acagaactcg
      661 aaagctaaaa tactctctca gccgtcaatt ataaccttaa ataatatgca ggcgattctt
      721 gataaaaacg taacgtttta tacaaaagtc tccggagaaa aggtggcgtc tctggaaagt
      781 attacaagcg gaacattgct gagagtgaca ccacgtatac ttgatgacag ctcgaatagt
      841 cttaccggaa aacgccggga acgagttcgt ttgttacttg atattcagga tggcaatcaa
      901 tcgactaacc aaagcaatgc tcaggatgcc agcagcacct tacctgaagt acaaaattca
      961 gaaatgacaa cagaagcgac attaagtgca ggagaaagtt tattacttgg cggatttatc
     1021 caagataaag aaagttcaag taaagatggt ataccgctgt taagcgacat tcctgttatt
     1081 ggtagtttat tctccagtac ggtaaaacaa aaacatagtg ttgttcgact ctttttaatt
     1141 aaagcaaccc cgataaaatc tgcatctagc gaataataaa tgaggcttta tgagcaggaa
     1201 atttagctct ctagaggata tttatgattt ctaccaggat ggtggcacat tagcgtcatt
     1261 aacaaatctg acacaacaag atctcaatga ccttcattct tatgcctata cagcatatca
     1321 atctggtgat gtaataaccg caagaaatct attccatttg ctcacatatc tggaacactg
     1381 gaattatgac tacaccttat ctctgggctt atgccatcag cgtttatcaa atcatgaaga
     1441 tgcacaactg tgtttcgcac gctgtgcaac tttagttatg caagatccca gggcatctta
     1501 ttattctgga attagctact tactcgtcgg aaataagaaa atggccaaga aagcctttaa
     1561 ggcttgttta atgtggtgta atgaaaaaga aaaatacact acatataaag aaaatattaa
     1621 aaaattgtta ggtaatacag agtaaaaact accatcagct ataccaatcc ccaggccgac
     1681 tggggatttt ctatcggaaa taataaagct gctatagaga agtgctccca aagttgagca
     1741 ccgaccactg gtagatttat ctacaggtgt ggggttattc cattaaccag gattttatgc
     1801 cctgaactat cttctgctgt atttaatatc atttccgttt atgaaacaat ctgtatatct
     1861 taacttagca ttagaaatct gatctttcca atcatctata gctcagagta tatttgtcag
     1921 gaaatgacct tcaaacttta gccgagaaaa aggcttaccc tggaaaacaa aacccttaaa
     1981 tatagcttta tttttattct tctataaaat atactcaaaa aattacgtct aactctcctt
     2041 tttccgcctc aagatcattt cgttccagat attagactgt cccacaatac cattaccaac
     2101 tcggtaggtt tttctttttg gtccggttga tccaggactt tctatcggaa ccgcctcaag
     2161 gttaacatca taaatgaaaa tctcaaggcg cttataatgc aaattaccat aactaacata
     2221 tctgacacga aatgaaattt tttcttttct tctcgatata taagttataa taaatgatgc
     2281 tctcctcagg tttataccga atgcttcggc aatatcattg cgagaaatcc atttctcctg
     2341 caatttacac caaagagaaa ccaagatata cagaggttca ccatcg
//
LOCUS       DAZFJL010000255         2369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFJL010000255 DAZFJL010000000
VERSION     DAZFJL010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.257"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(46..459)
                     /gene="hns"
                     /locus_tag="ACHJ8C_004641"
     CDS             complement(46..459)
                     /gene="hns"
                     /locus_tag="ACHJ8C_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO5554341.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            1063..1680
                     /gene="tdk"
                     /locus_tag="ACHJ8C_004642"
     CDS             1063..1680
                     /gene="tdk"
                     /locus_tag="ACHJ8C_004642"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO5554342.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            complement(1966..2369)
                     /locus_tag="ACHJ8C_004643"
                     /pseudo
     CDS             complement(1966..2369)
                     /locus_tag="ACHJ8C_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          595 a          524 c          547 g          703 t
ORIGIN      
        1 atcccgccga tggcgggatt tttagcatgt gcaatctaca aaagattatt gcttgatcag
       61 gaaatcgtcg agggatttac cttgctcatc cattgctttt ttgattacag ctggagtacg
      121 gccctggcca gtccaggttt tagtttcgcc gttttcgtca acgtagctat attttgccgg
      181 acgctgagca cgcttagctt tggtgccaga tttaacggca gcaaggctat tcagcagttc
      241 gttcgggtca ataccgtcag cgatcagcat ttcgcgatat tgctgcagtt tacgagtgcg
      301 ctcttcaact tcagcagcag ccgcgctttc ttcttcgcga cgttcgttaa caacaacttc
      361 taatttttcc agcatttctt ccagcgtttc aagtgtacat tctcttgcct gcgcacgaag
      421 agtacggatg ttgttcagaa ttttaagtgc ttcgctcatt gtagtaatct caaacttata
      481 ttggggtggt ttgttgaggt aataatagag ccttaaattc agttgtgcaa tagccaggaa
      541 tgtaaggaat tcaaaattgt tctttatttt gtgccgccaa taaatatctt ttcataaaat
      601 tagccagaaa agacgcggca tatagcccta tttgcaccga tgatttcgca gcacgtgagg
      661 ttaaaacttc ctgattcatg tcacatttta tggggagata atcgtaggct gacgaccttt
      721 tagtcttctg tattagccgt gtttacgaga attccctatt aagcgaatga tgaaaagtag
      781 aacagtcgca ataagagcag ggacttagta ttgcactatc tcctggaggt caacagaggg
      841 ctattacatg cgcaacaggt taaagattgt gaatagttac cggcagtcat ttacccgctt
      901 ataacaagcg aggcagttgt aatgatagct cagaaggatt atgcaaggct tcgtaaggga
      961 gaacgcatat accacttctg tgcatactgt tgagctgaaa aactgacgaa ttatgataaa
     1021 ctccagccaa ctttatttca tatcattgag ggcctgtggc tgatggcaca gctatatttc
     1081 tactattccg caatgaatgc gggtaagtct acagcattgt tgcaatcttc atacaattac
     1141 caggaacgcg gcatgcgcac tgtcgtatat acggcagaaa ttgatgatcg ctttggtgcc
     1201 gggaaagtca gttcgcgtat aggtttgtca tcgcctgcaa aattatttaa ccaaaattca
     1261 tcattatttg atgagattcg tgcggaacat gaacagcagg caattcattg cgtactggtt
     1321 gatgaatgcc agtttttaac cagacaacaa gtatatgaat tatcggaggt tgtcgatcaa
     1381 ctcgatatac ccgtactttg ttatggttta cgtaccgatt ttcgaggtga attatttatt
     1441 ggcagtcaat acttactggc atggtccgac aaactggttg aattaaaaac catctgtttt
     1501 tgtggccgta aagcaagcat ggtgctgcgc cttgatcaag caggcagacc ttataacgaa
     1561 ggtgagcagg tggttattgg tggtaatgaa cgctacgttt ctgtatgccg taaacactat
     1621 aaagaagcgt tagaagtcgg ctcattaacg gctattcagg aaaggcatcg ccacgattaa
     1681 taagaatttc tttactgaca gggtgagcag ggcgctttta tcctgtcagt tcgttttacg
     1741 cacttcttcc gggctataca cccttctcgg cagtttttta acgccgctat acgcctcaca
     1801 gagcgcttat acaccgacat tgacttgtaa cctgtaaagt acaatatcgc tgtgtgtagg
     1861 cgttatacat cgtcgcaaat atgatgaagg acaatgctgc cggtttatgg aaaagttgct
     1921 ttgggaaaac aaaaaaacgg ccccagaagg ggtaatgcca gtcagttaag caactgactg
     1981 gctctttttc ggggctgtgg ggtatttcca gggcctctcc tttaccactc tcgggaaggc
     2041 cctttccctt cttgtcggta atttcacaag ttgtcccata cttgcaagat cgcgcatcag
     2101 ctccggtata cgtcccggtg aagcgcatgg cgtccgtcat cgacgtcagc agatggcaga
     2161 cttttccttt gcgcgtcacg gtcagcaggc gggctgtcac ctcatttccc agtcccggcc
     2221 acttttttcg tgcctgcggg ctgatgttca gcttcaccag atgatcgcct ttacccagtt
     2281 ttctgatctc ttcatattgc gctccctttc tgagagggat catccagtgg cggtgttctc
     2341 ccgccaggct ccaggcattt aacagtcct
//
LOCUS       DAZFJL010000256         2353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFJL010000256 DAZFJL010000000
VERSION     DAZFJL010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.258"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..537
                     /locus_tag="ACHJ8C_004644"
     CDS             <1..537
                     /locus_tag="ACHJ8C_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFO5554343.1"
                     /translation="QTYDEQVNASWSEVLNQYQRRTDLIPNLVASIKGYSSHEQEVLE
                     AVTLARSQANRASSDLQKTPGDEQKLQAWQQAQAQVTRTLGQLTIISERYPQLKAQEL
                     YQNLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKFPAVLTAKVMDYTPKKNYLPDDV
                     TAVSKAPTIDFSQNANAH"
     gene            551..1222
                     /locus_tag="ACHJ8C_004645"
                     /pseudo
     CDS             551..1222
                     /locus_tag="ACHJ8C_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            1459..>2353
                     /locus_tag="ACHJ8C_004646"
     CDS             1459..>2353
                     /locus_tag="ACHJ8C_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO5554344.1"
                     /translation="MRIFLLLLMLCGFQVFAERLPSVTDLTGTLTTEEQSALNQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQRYGVYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEQMLPPEQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQLSLPCLAWTVLMIVAIIVLWRFTSRLGPGIWMISMITPTVWFFCFQ
                     DDVIIRRVSVVCFSLFFAAICWQRLAVVFNMCRVFPMMLAPKNKNAKVKKQKKTPQVR
                     ESGRNSLFVVMLIIIVGWCLVF"
BASE COUNT          651 a          528 c          551 g          623 t
ORIGIN      
        1 caaacctatg atgaacaggt taatgcctcc tggtcagaag tattaaatca atatcaacgt
       61 cgcaccgatc ttattcctaa tcttgtcgct agtattaaag gttactccag ccatgaacaa
      121 gaggtactag aagccgtaac attggcacgt agtcaggcta atcgcgccag tagcgatctt
      181 cagaaaactc cgggagatga acaaaaatta caagcctggc aacaggcgca ggcgcaggtg
      241 actcgcactc tcgggcagtt aacgattatt agtgagcgtt accctcagtt aaaggctcaa
      301 gagctctatc aaaacttaat ggtacagctc gaaggaagtg aaaatagaat tgccgttgcg
      361 cgaggaagat atattaaagc cattgaacaa tataatgtca ctatccgcaa attccccgct
      421 gtattaaccg caaaggtcat ggattacaca ccaaagaaaa attatttgcc ggatgatgtc
      481 accgcagtaa gtaaagcccc aacaatagat tttagccaga atgctaatgc tcattaagcg
      541 gggacgttct atgcgaaatt ttatcttcct tatggctttc ttctgttcat ctgtgtttgc
      601 tacacaaatt ccagtacctg aatcacccag gtatgtgaat gacttaaccg gcacattaac
      661 aaacagcgaa gtaaacaccc tgaccaacca gattaaagcc ctgacgcaaa aaaaccacgc
      721 acagttggtc gtgttagtgg ttgatacaac aggcgatgaa accatcgagc agtacgcaac
      781 gcgggtattc gatagctgga aaccgggtga taaagaccgt gatgatggcg ttctactgtt
      841 agtcgcctgg caggatcata ccgtgcgtat tgaaatcggt tacgggctgg aaggtattat
      901 tacggatgca cagtccggga aaattattcg caacagtatt attccggcat ttaaaaaagg
      961 cgacctggct ggtggtttac aaaaggggat caacgatatt gaaagtcgcc tgacggggaa
     1021 taattcagca acgataacac cgaccgatca ccctttgccc tttagcggtt ggtgggcttt
     1081 attagtctgg gcgataatac tgacgtttat ttctgccaga ggatatatca aaacgttggg
     1141 agtaatttgc ttcgcagcca ttgtactggc ctttgtttta cctatagcag gtttcagcgg
     1201 aagctgggga gtactcacaa cattactctg cttcgccaca ccgtttctgg cagttgccat
     1261 cgcttttacc cccttcggca aaaaggttcg ggattcgatg cgtgatgcca accagccatc
     1321 acgccataca agaagcaatt cttcctccca ctattcatca tcgtcatcct cttcattcga
     1381 caatgacaat tttagcggtg gtggcggttc atctggtggc ggtggcgctt ccgggcgctg
     1441 gtaacaaaag gaattgatat gcgtattttt ctcttacttc tgatgctatg cggttttcag
     1501 gtattcgctg aacgattgcc ttcagtgact gacttaaccg ggacattaac gacagaagaa
     1561 caaagtgctt taaatcaaca attacagact ctcgagcagc aaaaccactt ccaggtggcc
     1621 gtgttggtta cgccgaccac agggggcaaa aacattaaac ttgcagcctc gcagagatac
     1681 ggggtatatc actggaaacc cgttggagag aagcgatatg gcgaaggtat cttaattctc
     1741 gtcgtttggc cggaagggct ggcgtcaatg aagattggac atggactgga acagatgttg
     1801 ccgccggaac aagctgcgca aattgttcgt taccatatgc agccagaatt cgaaaaaaac
     1861 aatctctttg ctggattaac gggcggaatt gaaagcatcg cacagtttac ccatatcaaa
     1921 gcgaacctta gccctttgga tgctttagcc aatcatctgt ttgctaatcc gcaactttcg
     1981 ctgccatgcc ttgcctggac agtgctgatg atcgttgcca tcattgtcct ctggcgattt
     2041 accagccgtc taggacctgg catatggatg atatcaatga ttacacctac ggtctggttt
     2101 ttctgctttc aggacgatgt catcattcgg cgggtgtcgg tcgtttgttt tagtttattt
     2161 ttcgcagcaa tctgctggca gcgtctcgca gtagttttta atatgtgccg cgtatttcct
     2221 atgatgctgg ccccaaaaaa taaaaacgct aaagtgaaaa aacaaaagaa gactccgcaa
     2281 gttcgtgaat caggacgaaa ttcattgttt gtcgtgatgc tgataattat tgtgggatgg
     2341 tgcctggtgt tcg
//
LOCUS       DAZFJL010000257         2350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFJL010000257 DAZFJL010000000
VERSION     DAZFJL010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.259"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..682
                     /locus_tag="ACHJ8C_004647"
     CDS             <1..682
                     /locus_tag="ACHJ8C_004647"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO5554345.1"
                     /translation="TPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQAGNV
                     GRAVTKLPLMGKSLHKTIEKNEVKTAKKLPGPVPALVITAFVARRLLRFRHMLACRRR
                     GLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKL
                     NVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEKVLVDAEKAITD
                     FMTARGYH"
     gene            complement(731..2230)
                     /gene="pitB"
                     /locus_tag="ACHJ8C_004648"
     CDS             complement(731..2230)
                     /gene="pitB"
                     /locus_tag="ACHJ8C_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO5554346.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLNGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRQKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT          629 a          614 c          574 g          533 t
ORIGIN      
        1 aacacctcca ttaattgccg ttattggtag cgatggttca ggcaagtcaa cggtgtgtga
       61 acatcttatt accgttgtcg aaaaatatgg tgctgccgaa agagttcatt taggaaaaca
      121 ggccggaaat gtcggtcgtg cagtgacaaa attaccgttg atgggaaagt ctctgcacaa
      181 gactattgaa aaaaatgaag tgaaaacggc aaaaaaattg ccgggtccgg ttccggccct
      241 ggtgatcacc gctttcgtcg cccgccgact gttacgcttt cgtcatatgc tcgcctgtcg
      301 tcgtcgcggg ctgattgtct taaccgatcg ttatcctcag gaccaaattc ctggcgctta
      361 cgatggtacg gtgttcccac ctaacgttga aggcggccgg tttgtctcat ggctggcaag
      421 ccaggaacgt aaagcatttc actggatggc gagccataag cctgatctgg tcatcaaact
      481 caatgttgac cttgaagttg cctgcgcgcg taaacccgac cataaacggg aatcgctggc
      541 gaggaagatt gccataacgc cacagttaac ctttggcggt gcacaactgg ttgatatcga
      601 cgccaatcag ccactggaaa aggtgctggt tgatgcagaa aaagcgatta cggattttat
      661 gaccgcgcgt ggttatcact agtcaaaaat ggaaatgccc gatcgccagg accgggcatt
      721 ttcaggaagg ttaaatcaac tgcaatgcta tccagtacaa cccaccagaa agaaaaattg
      781 ccgccggtaa agtaaacacc cacgccatca ggatgctggt taccgtttta cgctgtaacc
      841 caccgccgtc caccaccatc gtccctgcta ccgcagacga gaggacgtgt gttgtggaga
      901 cgggcatccc aatataactg gcaaggccga tagacactgc cgccgtcatt tgtgccgcca
      961 tgccttgcgc atacgtcatg ccgcgcttac caatcttctc accgatggtc atcgctacac
     1021 gacgccagcc aatcatggtg ccaatgccga gcgccagagc tactgccatg atgatccaca
     1081 ccggagcgta ctcaatggtg cttaacatat cgctgcgcag ttttttcagc aggttctggt
     1141 cttctttact gacgcctggc agtttcgcca gcttcgcgga ggtatcagag atgcacagca
     1201 taatgcggcg cagctggctg cgctgactca cgcttaacgg ctcgtaactt tccatattgc
     1261 ctggcagcat cgttttaacg cgcgcaatcg catcaaaggt atttgccgga tgacagtgaa
     1321 actccgttac ttgcgtgcca tcagtcgatg ccgcaggcaa tggaggttcc atcgcaatca
     1381 acttctgcgg cagttcagga tgctgttgca ggtagtgttc gaagttggtg acggcatcgc
     1441 gggtacgggt aatttcatag ccggacgcat tcatattgac gacgaaacca gcaggggcaa
     1501 tccccaccag taccagcatc accaggccga tccctttctg cccgtcgtta gcaccgtgcg
     1561 aaaacgccac gcccgcagcg gaaacaatca gcgcaatacg cgtccagaat ggtggcttac
     1621 gtttgccttt tttcttttgg cgatcttccg gaatgcggtg aatacggtca cgctttttcg
     1681 tcccgctcca gtagcgacgc agcagaaaaa tcagcccccc cgcaatcacc agccccacga
     1741 tgggggagac aatgagtgaa gagaaaatct tggtgacttc gcgcaggttt aacgcatcca
     1801 tcaccgacga accatttaac agcgcgttgg ttaaaccgat gccgataatc gcaccaatca
     1861 aagtgtgcga actggaggcc ggtaaaccaa agaaccacgt tcccagattc cagataatcg
     1921 ccgccagcag catggaaaag accatcgcca gaccgtgagt tgaccccata ttcagcaaca
     1981 aatcggttgg caacatatgg acgatggcat aggcaacgct aagcccgcca agcaatacgc
     2041 caaaaaagtt aaaaaatgcc gccatgacta cagcaagctg tggttgcatg gcacgggtat
     2101 aaataacggt tgccaccgca ttcgctgtgt catgaaaacc attgattgct tcatagaaca
     2161 acacaaatgc cagagcaagt aataacaaaa gccccgtata tatatcaagg ccaacaaata
     2221 aatttagcat atattagatt acgccatttt gaatttacga acggaggcat tatcagtgac
     2281 tttcacggcg tgggcaaagt gaaatatcat tttttaacga taaaaagcgt gttatttctt
     2341 gtgtatcaga
//
LOCUS       DAZFJL010000258         2253 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFJL010000258 DAZFJL010000000
VERSION     DAZFJL010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.260"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            66..602
                     /locus_tag="ACHJ8C_004649"
     CDS             66..602
                     /locus_tag="ACHJ8C_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFO5554347.1"
                     /translation="MKTKIAIASDDVGFARKEEIKKYLIEEKNMEIVYDPVTCKEQGV
                     NNFARLADEMAGIIQRDECRLGIYICGTGIGFTCQINKHWGIRAVAVTNPYSAKRARL
                     SNNAQVIGLGCRVNDLEYSKMIVDAWLDNPFDFTTARENSKKNLLEAERNDNALLVKP
                     ADVRWNMGFRPDDEKSEG"
     gene            606..1976
                     /locus_tag="ACHJ8C_004650"
     CDS             606..1976
                     /locus_tag="ACHJ8C_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFO5554348.1"
                     /translation="MELITQFINDLGNFIFIPVIFLVLMKILGRPLSECISSAIKVGI
                     GFIALTMTIKLMLEKMQPAVTGLAEATGSSLSAIDVGGAATAVMGFGSSMGAIIIPLC
                     VAVNIAMLVARLTDCVNVDVFNLHQNASMGAIVGVYSGSFLYGALTAALFHVWALIAA
                     DLGAKNNEKFFNLPKGVAISHPVANTYLLFAYPFNWIYDRIPGFRNLNVTAETIQKRF
                     GILGDPTMVGFIIGILLGFCGYGWKSPYHTIIASLQLGMYLAAVMLLLPRMTSIMMEG
                     LVPLSNVARKKLVKRFPDRQITVGMDTALIVGHPSVIAPALLLIPVIVILAVILPGNR
                     VMPLGDLSQFVFFIACMVPVFNGNIIRTWVTSIILFGGGLYIASWMAPATNEVFQKFG
                     TNPDASVMYSSLNPSANPFTGLFAALSHVGIIGYVMAGILLLSIGYLIKQKSRRQIET
                     DLEKAL"
     gene            2000..>2253
                     /locus_tag="ACHJ8C_004651"
     CDS             2000..>2253
                     /locus_tag="ACHJ8C_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO5554349.1"
                     /translation="MKKILVVCGNGIASSSIMVSVLQDFLKEEKIEAQVDKSSLMACT
                     SDTFNSYDLVVSSTKLDNPAITTRVIVGAGLLTGLGEDEI"
BASE COUNT          578 a          513 c          522 g          640 t
ORIGIN      
        1 aattgacaac caatatttac caacagcata cttggcttta gttacttaac gacaggcaaa
       61 atgaaatgaa aacgaagatt gctatcgcaa gcgatgatgt aggttttgca cgtaaagagg
      121 agataaaaaa atacctcata gaagagaaaa acatggagat cgtgtacgat cccgttacat
      181 gtaaggaaca aggtgtcaat aacttcgcgc gtctggccga cgaaatggca ggcattattc
      241 aacgcgatga gtgccgtctg ggaatttaca tttgtgggac tggcattggc tttacttgtc
      301 agatcaacaa acactggggc ataagagccg ttgcggttac taacccctac tccgcaaagc
      361 gggcgcgact cagcaataac gcacaagtta ttggactggg ttgccgggtc aacgatctcg
      421 aatacagcaa aatgattgtc gatgcatggt tggataaccc attcgacttc accaccgcca
      481 gagaaaattc taaaaagaac ttactggaag ctgaacgtaa cgataatgca ttgctggtta
      541 agcccgctga tgtacgctgg aatatgggtt tccgaccaga tgatgaaaaa tctgagggtt
      601 aatgtatgga actgatcacg caatttataa acgatctggg aaattttata tttatcccgg
      661 tcatctttct ggtactgatg aagatacttg gccgtcctct ttcagaatgt atctcctccg
      721 ccatcaaagt cggcattggt ttcattgcgc taaccatgac catcaaactg atgctggaaa
      781 aaatgcaacc ggcagtcacc ggattagcag aagcaacagg ttcctcgctc agtgccatcg
      841 atgttggtgg cgcagcgact gcagtgatgg gatttggctc cagcatgggc gctatcatta
      901 ttcccctctg tgttgcggta aatattgcaa tgctggtcgc ccgcctgact gactgtgtta
      961 acgttgatgt tttcaacctt catcaaaatg cgtcaatggg ggcaattgtt ggcgtctatt
     1021 ctggtagctt cctgtatggc gcattgactg ccgcgctatt ccatgtctgg gcgctgatcg
     1081 ctgccgatct cggtgctaaa aataacgaaa aattctttaa cctgccaaaa ggtgttgcga
     1141 tctctcaccc ggttgccaat acctacttac ttttcgctta tccattcaac tggatttatg
     1201 atcgcatccc aggcttccgt aatctgaatg tgaccgccga aactattcaa aaacggtttg
     1261 gcattctggg cgatccaact atggttggtt ttattattgg tattttgttg ggcttttgtg
     1321 gttatggctg gaaatccccc taccacacca taatcgccag cctacagtta gggatgtatc
     1381 tggctgcggt catgcttctg ttgccacgta tgacctctat catgatggaa gggcttgttc
     1441 cgctttccaa cgtggcacgc aaaaaactgg tcaaacgttt cccggatcgt caaatcactg
     1501 ttggtatgga cactgctctg attgtgggtc atccatcagt tatcgcccct gcattattac
     1561 tgatcccggt cattgtgatc ctcgccgtga tcttgcccgg caaccgcgtt atgccactgg
     1621 gtgacctctc tcagtttgtg tttttcattg cctgcatggt acctgttttc aatggcaaca
     1681 ttattcgcac ctgggtgacc tcgatcattt tgttcggtgg tggtctgtat attgcatcgt
     1741 ggatggcacc ggctaccaac gaagtcttcc agaagtttgg tacaaacccg gatgccagcg
     1801 tgatgtactc ttcgcttaac ccgtctgcga atccatttac tggtctgttt gccgccctga
     1861 gccatgttgg aatcattggc tatgtgatgg caggtatcct tttgttatct attggatact
     1921 taattaaaca aaaatcacgt cgccagattg aaacggattt agaaaaagcg ctttaaatct
     1981 tttacagagg aataatcaaa tgaaaaaaat cttagtagtt tgcggaaacg gaattgcctc
     2041 atcttccatc atggtctctg tactgcagga cttcctgaaa gaagagaaaa ttgaagcgca
     2101 agtcgataaa tcttcgttaa tggcatgcac cagcgatacc ttcaacagct atgacctggt
     2161 ggtttcatcg accaaactcg ataacccggc cattactact cgtgtcatcg ttggcgctgg
     2221 attactgact ggtctgggcg aagatgagat ttt
//
LOCUS       DAZFJL010000259         2234 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFJL010000259 DAZFJL010000000
VERSION     DAZFJL010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.261"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..470)
                     /locus_tag="ACHJ8C_004652"
     CDS             complement(<1..470)
                     /locus_tag="ACHJ8C_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO5554350.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQIEFNT"
     gene            complement(522..2204)
                     /gene="ydeN"
                     /locus_tag="ACHJ8C_004653"
     CDS             complement(522..2204)
                     /gene="ydeN"
                     /locus_tag="ACHJ8C_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5554351.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQNKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDNSQPPLSEVNQEKFNNIKKALSEAK"
BASE COUNT          580 a          468 c          498 g          688 t
ORIGIN      
        1 aacgtattaa actctatttg atatgatttc agacgctcga tggctgctat cacttttgca
       61 aaagtaccgt tacctgaatt actgcgtctg tagcggtcat gtagttcttg gggtccatcg
      121 atcgagatac caaccagaaa ctcatgttct ttgagaaagg cacaccattc attattcaat
      181 aaaatgccat tcgtttgcaa tgcattaaaa attcgttttt ggcctgcata gcgttgttga
      241 tagtgaataa ctttacggaa aaaatccagg ccagccagag tgggttcacc gccttgccag
      301 gtaaaataga cctgattgcc agacgctgcg atatattgtt tgatgaactc tttcagagtg
      361 ctgtcatcca tccatttttc atgagtaaac tgcgactctt tttcaaggta aaaacagtaa
      421 tcacatttga gattacattg aaaactggag ggcttggctg taacgtgcat cgctatctcg
      481 ctcaataagg cggcggaaaa atccgccgca tgaaggttta gttatttcgc ttcgcttaat
      541 gctttcttga tattgttaaa cttctcctga tttacctcgc taagcggtgg ctggctgttg
      601 tcgataaact ctcttaccac gccttgcatc tctttaacga cctgcggatt ggcggcggca
      661 aggttatctt tttgctgtag atccgtcagt ttgtatagac ctaactgatt attttctact
      721 gtatagacaa gcgaataatc gttatttctc accgtataag agaattggct taagtcctcg
      781 gtgttggggt tatgcgggta atcgtctgac tgatggcgga caaatttgtg gtaattatcc
      841 cagaatggaa tattttcctc atcaaaccag tgagaataag atgttatcca ggtcagattt
      901 ttatgtggct cgccttgttt cttattttgc aaccagggca gcaaggaaac gccatccagc
      961 ttaaggtctt ttggaatgct gatatcggct gcatcaagag ctgtcgggta gaaatccatt
     1021 gcggatatca gcttgtcata attaccgggt tgaagttttc ctttccacca cataaacatt
     1081 ggggtgtgag taccgccagg ataggtctga ctcttatagc ctttttgcgc cccgttcagc
     1141 ggcagaggac catcgataac tgcaccatta tcggaggtaa agagaataat tgtattgtca
     1201 tactgtccgt ttttcttcag ttgttcgaga atgcgtttta caccctgatc aacagaataa
     1261 acggaagcgt agtaattatc tgctgtttga ctaccggtat taaattgctt ctgatattgc
     1321 tccggagcag gattatcatt tggcaggtgc ggagcattat aagccaggta aagcataaaa
     1381 ggctggtcaa gtgttttggc acgatcaaca acgccaattg cctcatcggt taactgatcg
     1441 ctgatataac cttttgcggg gacgcgttca cggtttttga acagtgaagg ggagttgtaa
     1501 tatgccgttc ctgcagcatg gaatcccata aagtaatcaa agccacggtt ttgcggttgc
     1561 cattcttccg cagaaaaggt agtgaagttg tcatgatagt cacgcgtttg tttatcttcc
     1621 ggcaccggca cattactgat ttttgataag tgccatttac ctactgctgc agtgtaataa
     1681 ccatgattct ggaataattc aggcaagaaa gtttctgtta gcggaatacc atcctgagca
     1741 tcagtattgg aatagacacc aaagcgggcg ggcgctcgac cggtcattat tgcggcgcgg
     1801 gaggggccgg aaacaccgtg cgccacatag ccgttagtaa aacgtacgcc ttcatccatt
     1861 aatgaaagga gcgtcggcgt tgatttttgc gcagcttcaa tggctttatc tatccctatt
     1921 ttgtaggtat cgacaacttc acgattttcc attgtttttg ggtcaaaaga tcccttatca
     1981 aaaggaagtt gtccataacc aagatcatcc atggtcagta cgataatatt tggctttcct
     2041 ttggtactgt attcagtcgg cgtaaagtct gagaaagcaa cgtttgtttt ggttgctttc
     2101 agctttacat catctgccgc atgagcagca aatgcagcca taccagatgc cagtatcaaa
     2161 gatatcgagg tacttacgac acttttcttt aatgcagact tcataaatgt tcctcttctt
     2221 attgtactta attc
//
LOCUS       DAZFJL010000260         2193 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFJL010000260 DAZFJL010000000
VERSION     DAZFJL010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.262"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..876
                     /locus_tag="ACHJ8C_004654"
     CDS             <1..876
                     /locus_tag="ACHJ8C_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5554352.1"
                     /translation="TNAPAEDPDIPASGYYGAASRTNGNQVSSNRPTHFSSWARRSII
                     PDRLATAILNAAGRTSAFISGKAPEIKPSPGGNTPSGPSADTSVRTISLLPAAGEAAA
                     QGWSIKDGGIQLSDGVFKITKQSNKTWSLTHPVDDAITLLTQGGRLTCKFRLSGALTN
                     NQFGLGIYLYTDAPVPDGVAMTGTGNPFLMSYFTQTTDGRVNLMHHRKAGNTKLGEFG
                     DYGNDWQTLELVFTAGSATVTPKLNGVAGPAFQVIKDSLTLGLNALTLTDVTKNAAYG
                     VEIESLMLEINAPAA"
     gene            1315..1530
                     /locus_tag="ACHJ8C_004655"
     CDS             1315..1530
                     /locus_tag="ACHJ8C_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5554353.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            1530..2027
                     /gene="rrrD"
                     /locus_tag="ACHJ8C_004656"
     CDS             1530..2027
                     /gene="rrrD"
                     /locus_tag="ACHJ8C_004656"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO5554354.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYINVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKSACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            2024..>2193
                     /locus_tag="ACHJ8C_004657"
     CDS             2024..>2193
                     /locus_tag="ACHJ8C_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO5554355.1"
                     /translation="MSRVTVIISALVICIIACLSWAVNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAEL"
BASE COUNT          571 a          516 c          598 g          508 t
ORIGIN      
        1 actaacgcgc cggcagaaga tccggatatt ccggcatcag gatattacgg tgcggcatcg
       61 agaacgaatg gaaaccaggt atcatcaaac cgcccgacac atttcagttc atgggcgcgc
      121 aggagcatta ttccggatcg tctggcaacc gctattctga acgcagccgg gcgcacctca
      181 gccttcatca gtggtaaggc accggaaatc aaaccctcgc ccggcggcaa cacgccatcg
      241 ggtccgtctg cagatacgtc cgttcgcaca atctccctgc tgccggcagc cggagaggct
      301 gctgcgcagg gctggagcat taaggatggc ggaattcagt tgtcagatgg tgtatttaag
      361 atcaccaagc agagcaataa aacctggtcc ctgacgcatc cggtggatga cgcaattacc
      421 ctgctgacac agggcggcag actgacctgt aagttccgcc tgtcaggcgc actgaccaac
      481 aatcagttcg ggctggggat ttatctgtat acggatgctc ccgttcctga tggtgtggcg
      541 atgacgggta ccggtaatcc gttcctgatg tcgtacttca ctcagaccac tgacggcaga
      601 gtgaatctga tgcatcacag gaaagccgga aacacgaagc tgggggagtt cggcgattac
      661 ggtaacgact ggcagacgct ggagctggtg ttcaccgccg gcagtgccac ggttactccg
      721 aaactgaatg gagtggctgg cccggcattc caggttataa aagacagtct gacactggga
      781 ctgaatgcgc tgacgctgac ggatgttaca aaaaatgcag cgtatggcgt tgagatagaa
      841 agtctgatgc tggagataaa tgcaccggca gcataataaa aaaagccagc gactgacctg
      901 aaaaagaaga cgctggctaa aaggccttat atgtttgtag agacttattt ttcacagaca
      961 gcaatgatgc ctgtcaatat attatcaata tgcggattgt ttcagttaca gatgccttat
     1021 taaggaaaaa aacagccagc actgactttc ggtggagagg tgctggctca aaaggataga
     1081 tgtacttcac atgttgcttc tatatggcag tacattttct gacagacagt gacggatgtt
     1141 gtcaagatat tgtgtcattt ataacctgaa tcaggggagg ccggaatgtt atctggcatt
     1201 tttagcagag cctgaatgcc ataatcacgg ctcccggcgt tggccgtcag tgggtgacac
     1261 tggcggcttt tttgtttttc tttactttca ttttctgtcg gcggtgacgg agacatacat
     1321 cagatggaaa aaatcacaac aggtgtgtca tacaccacgt cagcggtggg gacgggatac
     1381 tggttactgc agctgctgga caaagtctct ccgtcccagt gggtggcaat aggtgtgctg
     1441 ggaagtctgc tgtttggcct gctgacgtat ctgacaaatc tttatttcaa gattaaagaa
     1501 gataagcgta aggctgcgag aggtgaataa tgtcgccgtc attacgcaag gctgttgcag
     1561 ctgctattgg tggtggggct gttgccatag cgtctgtgct catcactggt ccaggtggta
     1621 acgatggtct ggaaggtgtc agctacatac catacaaaga tatcgttggc gtatggactg
     1681 tatgtcacgg acacaccgga aaagacatca tgcccggtaa aacgtatacc gaagcagaat
     1741 gcaaagccct cctgaataaa gaccttgcca cggtcgccag acaaattaac ccgtacatca
     1801 acgtcgatat accggaaaca acgcgcggcg ctctttactc gttcgtttac aacgtgggcg
     1861 ctggcaattt cagaacatcg acgcttcttc gcaaaataaa ccagggcgat atcaaaagcg
     1921 catgtgatca gctacggcgc tggacatacg ctggcggtaa gcaatggaaa gggctgatga
     1981 ctcgccgcga gattgagcgt gaagtctgtt tgtgggggca acaatgagca gggtaaccgt
     2041 tattatctcc gctctggtta tctgcattat cgcctgcctg tcatgggctg ttaatcatta
     2101 ccgtgataac gccatcgcct acaaagagca gcgcgataaa gccacatcca tcatcgctga
     2161 tatgcagaag cggcaacgtg atgtagcaga act
//
LOCUS       DAZFJL010000261         2184 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZFJL010000261 DAZFJL010000000
VERSION     DAZFJL010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.263"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..643
                     /locus_tag="ACHJ8C_004658"
     CDS             <1..643
                     /locus_tag="ACHJ8C_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HFO5554356.1"
                     /translation="HFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPT
                     AYGKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGD
                     IYECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCVYKPICNGGCPKHR
                     ITKVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(609..734)
                     /locus_tag="ACHJ8C_004659"
     CDS             complement(609..734)
                     /locus_tag="ACHJ8C_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554357.1"
                     /translation="MKTKHPTGIYVGCLVKVIRRRLRMELRGSVINYSPFVLQHP"
     gene            748..1911
                     /locus_tag="ACHJ8C_004660"
                     /pseudo
     CDS             748..1911
                     /locus_tag="ACHJ8C_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M16"
BASE COUNT          666 a          469 c          446 g          603 t
ORIGIN      
        1 tcatttttta aaatctatcg gcagtaaaca tatgcaattt atcgaattgc tcgaaaccgg
       61 gacgccgaat attgatttca gtggtcatag tgagaacaca ttccgtatca ttgatttttc
      121 tgtgcctccc acggcttatg gcaagtttat gtcaaccatt tttatgcaat gggttaaaaa
      181 cgatgtgggt gaaattttca tccgtcagtt tgaaagtttt gtcagccgtt ttttggggaa
      241 tgggcatacc agttgtattt tccaggagtc ctgcaaggat aatctggttg ttgaaagtaa
      301 tggagacatt tacgaatgcg accattttgt ctatccacag tacaaaattg gaaacattaa
      361 taaatcggaa ctcaaaacga tgaacagtgt acaactgaca gcgcaaaaaa aacggatttc
      421 agcgaaatgt cagcaatgtg tatataaacc tatctgcaat ggtggttgcc ctaagcatcg
      481 tattactaaa gtaaacaatg agactgtttc ctatttttgc gaaggttata aaatcctttt
      541 ttcaaccatg gtaccttata tgaacgccat ggtagagtta gctaagaaca gagtaccgct
      601 ttaccacatt atggatgttg caaaacaaat ggagaataat taataacgct cccccttagc
      661 tccattctta gcctccttct tatgacctta acaaggcacc cgacataaat tcctgtcggg
      721 tgtttcgtct tcatcatctt cctttgcgaa attattgccg tatctaataa aggcgattta
      781 agtttccctg cgcagcaaaa atcacttatc gcgctggcta ataaagcagt aagcggaagc
      841 ggcgttggtg aactctcctc ttccagcctg aaacgctgga gtgcggaaaa ctcggtaacc
      901 atgagcagta aagtcagtgg catgaatacg ttgctctccg ttagcgcgcg gactaataac
      961 cctgaacctg gtttccagtt gattaaccag cgaatcaacc acagcacgat taacgacaat
     1021 atttgggcat cgctacaaaa tgctcaaatt caggcgttga aaacgctcga ccagcgtcca
     1081 gcggagaaat tcgcccagca gatgtatgag acgcgctatg ccgatgaccg cacgaaatta
     1141 ccgcaagaaa atcagattgc gcagtttact gccgcagatg cgctggctgc cgatcgccag
     1201 ttgttttcat ctccagcgga tatcacgttt gtcattgtcg gtaatgtcgc agaagacaaa
     1261 ctcgtggcgt taattacgcg ttacttagga tcaatcaaac actctgattc gccattagcc
     1321 gcaggtaaac cattaactcg cgcgacggac aacgcatcgg ttactgtaaa agaacaaaat
     1381 gaaccagtgg cacaggtttc acagtggaag cgttataatt cccggacacc tgttattctg
     1441 gcaacgcgta tggcgctcga tgcctttaac gtcgcactgg caaaagatct acgcattaat
     1501 attcgtgaac aggcatctgg ggcatacagc gtttcttctc gcctctcggt tgatcctcag
     1561 gccaaagata tcagtcattt gctggctttt acttgtcaac cagaacgaca tgatgaactg
     1621 ttaacgttag cgaatgaagt gatggttaag cgtctggcca aagggatcag tgagcaagaa
     1681 ctgaacgaat accagcaaaa cgttcagcgc agcctcgata tccaacagcg tagcgttcaa
     1741 caattagcga acaccattgt aaatagtctt attcaatatg acgatcctgc agcatggact
     1801 gagcaggagc aattgttgaa acaaatgacg gtagagaatg ttaacactgc tgttaaacaa
     1861 tatctttctc atccggtgaa tacctatacc ggagtattat tgccaaaata atcacagccc
     1921 cgtcaacaca tcgttggcgg ggattttatc aatattcgct atttttatgt aataacttta
     1981 taaatgtgtt caaagtaaat aaccgtgtac taataatgat attataaggt ctgattttta
     2041 cgtgataatt caggagacaa tggaaagcac ataaaaataa cagcataaaa caccttacct
     2101 ccacacagaa tcctctatta tgttgttttt taataaaaat acattattca cgcaatatct
     2161 cgcgataaat aacattagga tttt
//
LOCUS       DAZFJL010000262         2047 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZFJL010000262 DAZFJL010000000
VERSION     DAZFJL010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.264"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..158)
                     /locus_tag="ACHJ8C_004661"
     CDS             complement(<1..158)
                     /locus_tag="ACHJ8C_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554358.1"
                     /translation="MLSPLSGFQPPSSFEFRDPPQRREARFAKRRCPLHPGGLLPGCQ
                     ERRPRRDRG"
     gene            160..446
                     /locus_tag="ACHJ8C_004662"
                     /pseudo
     CDS             160..446
                     /locus_tag="ACHJ8C_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            565..1386
                     /locus_tag="ACHJ8C_004663"
     CDS             565..1386
                     /locus_tag="ACHJ8C_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5554359.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(1681..>2047)
                     /locus_tag="ACHJ8C_004664"
     CDS             complement(1681..>2047)
                     /locus_tag="ACHJ8C_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO5554360.1"
                     /translation="VNAIGINPVTGYGSGLMQVDSQHFNELARYGIKPEHLTTDPCMN
                     IYTGAYYLAIAFKKWGVSWKAVGAYNAGFRKSERQNQRRLAYASDVYRIYTGIKSSKG
                     IRIPATKKSLPEINSVQNN"
BASE COUNT          531 a          446 c          519 g          551 t
ORIGIN      
        1 cctctgtcgc gccgtggcct gcgctcctgg catcccggca aaaggccgcc gggatgtaag
       61 gggcaacgcc gtttcgcaaa ccgcgcttca cgtcgttgcg gcgggtcccg aaactcaaaa
      121 gatgatgggg gctgaaaacc agagagagga gaaagcagga tgagtacccg aaatatccac
      181 gttaacaccg cgtcgtacac gattcttgtt gcctggaaga aaaaaacacc ggggaagagt
      241 gggacgtcct ggaattcagc agcctgactg agctgaaaaa atatcgcaaa agccacccgg
      301 aaaagatggc cttcagctat agctacgccc tcagccgggg tgtggatacg cagttccgcc
      361 atatcaacat tgcggaagcc gatcacttca aacagttcct gcgtcagatt aagcgtgccg
      421 gcctcgatat ccgggcgatc tgctgaccgg atgtgatgaa tatccctcct gattgttgca
      481 atcttgttaa tggtgaccag gggggatgat attaagcgga aaaatttttt attaaattta
      541 ttctttcata aaggagtagc tgttatgcga ttagcttccc gttttggtcg gtataactcc
      601 atccgccgtg aacgtccttt aacggatgat gaattaatgc agttcgtgcc ttcggtattt
      661 tccggtgata aacatgagtc ccggagtgaa cgttatacgt atattccaac aatcaatatc
      721 atcaataagt tacgtgatga aggtttccag ccattctttg cctgtcagag tcgggttcgt
      781 gatttgggac gtcgcgaata cagtaaacat atgttacgtc ttcgcaggga agggcatatt
      841 aacgggcagg aagttcctga aattatcctg cttaattcac atgatggttc atccagttat
      901 cagatgatcc ccggaatttt tcgttttgtc tgcacaaatg gcctggtgtg cggaaataat
      961 tttggcgaaa tccgcgttcc acataaaggt gatattgtcg ggcaggttat cgagggagcg
     1021 tatgaagtac tcggtgtctt tgataaggtc actgataata tggaggcgat gaaagaaatt
     1081 catcttaaca gtgacgagca acatttattt ggcagagctg cactgatggt caggtatgaa
     1141 gatgaaaata aaacgccagt gacgcctgaa cagataatta ctccccgtcg tcgggaggat
     1201 aaacagaacg atctctggac aacctggcag cgggttcagg agaatatgat aaaaggtgga
     1261 ttatcggggc gaagtgcctc cgggaaaaat accaggacaa gagccattac aggtattgat
     1321 ggtgatataa gaatcaacaa ggcgttatgg gtgattgccg aacagttcag aaagtggaag
     1381 tcatgataat ataagtaaag ccccgaaaaa ttttcggggc tttacttata ttatcattct
     1441 tcggcatcgc tgtgtcgtct gatatacctc aatttcatca gctggctttc cggttgtctg
     1501 taaatattgt gcccgcgaaa atggtctggc aggaggctgc gcagttaccg ggtatatata
     1561 agatgggttc ccgcttcgcg gggcttcggc cagtcagttt tgtcactcat atgatatttt
     1621 tgtgtgtggc cttccatgcc gcttttgcgg cataaccggt atctgacaat gtctgtaaga
     1681 ttaattgttc tgcacactgt taatttccgg aagtgatttc ttcgtggccg gaatccggat
     1741 acctttactg ctttttatcc cggtataaat ccggtaaaca tctgatgcgt aggcaagacg
     1801 tctctggttc tggcgttcgc tcttcctgaa tccggcattg tatgcaccaa cggccttcca
     1861 ggagacgccc cattttttaa aggctattgc cagataataa gcaccggtat aaatgttcat
     1921 gcagggatct gttgtcagat gttccggctt aattccatag cgggccagtt cgttaaaatg
     1981 ctgggaatct acctgcatca gtccgctgcc atatcccgtt accggattaa taccgatggc
     2041 attaacc
//
LOCUS       DAZFJL010000263         2008 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFJL010000263 DAZFJL010000000
VERSION     DAZFJL010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.265"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..1855
                     /locus_tag="ACHJ8C_004665"
     CDS             <1..1855
                     /locus_tag="ACHJ8C_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5554361.1"
                     /translation="TIDEIRQKTSAMSLPEAADNEEKTRQALEEQNRLIGEQEGKIRG
                     LKNQIADYQRWLDESPQSGTSAGIIRKGLAEATSQLAVEQSRLTQMQEKAQSIQEVLA
                     RLEYQRVAKIREEAAEQNKAYQSLLRMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRL
                     PQATLDDKQQTALNNSERELALSRLKGEARERARLGYAADDLGFVGEAYQTARQNYIN
                     NSLDAWRNNQANKPKAHKKTEAEKTEDIYKRLIKQQKEQIALAGQNTELAKMKYQVSQ
                     GELSTLSEAQKKTLLQNAALIDQKKIREQLAAYESSLADSNASARASDDAQLLGYGEG
                     SRMRERLQEMWSIRQAFEQKNNELLRQYQAGEIEEALWKQEKELNKKYLEERLSDQQD
                     YYAKADALRNNWNAGLQEGLTNWADSATDYASQAADAVVSTMDGLVSNISDALAGNVV
                     DWRNWGSSVLREVSKILMNAAIVNGLKSLSGAGGWLGTVGGWISGAVANAKGGVYTSA
                     NLSAYSNTIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAIGNVNGGSGF
                     VYSPVYHISIQNQGCNGEIDARSARGLVDLIDSRVVSIMQSSRRDGGLCSA"
     gene            1848..>2008
                     /locus_tag="ACHJ8C_004666"
     CDS             1848..>2008
                     /locus_tag="ACHJ8C_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5554362.1"
                     /translation="MPESEVFNWIPREGMETTRKPSVITVKFGDGYEQRRAGGLNADL
                     KTFKPVFRVT"
BASE COUNT          576 a          430 c          593 g          409 t
ORIGIN      
        1 aacaatcgac gaaattcgcc agaaaacgtc ggcaatgtca cttcctgaag cggcagataa
       61 tgaagaaaag acgcgacagg ctctggagga gcagaatcgc ctgattggtg agcaggaagg
      121 aaagattcgt ggactgaaaa atcagatagc agattatcaa cgctggcttg atgaaagtcc
      181 gcagagtgga acttccgcag gcatcatccg gaaaggactt gctgaagcaa caagccagct
      241 ggctgttgaa cagtcccgtc tcacacaaat gcaggaaaaa gcacagtcca tccaggaggt
      301 gcttgcccgg ctggagtacc agcgtgtggc gaagattcgc gaagaggcgg cggaacaaaa
      361 caaagcgtat cagtccttac tgaggatgaa tggccaacat accgagttta atcgtcttct
      421 cgggctcggt aatgaattac ttcagcagcg acaggggctg gtgaatgtac cgttacggct
      481 accacaggca accctggatg ataaacagca gaccgcactg aataacagcg agcgcgaact
      541 ggctctgtcc cgcctgaagg gggaagcccg tgagcgtgcc cgcctgggtt atgctgcgga
      601 tgatctcggc tttgtgggag aggcgtatca gacagccaga cagaattata tcaataactc
      661 actggatgcc tggcgaaata accaggcaaa taaacccaaa gcgcataaaa agaccgaagc
      721 ggaaaaaaca gaagatattt ataaacggct gattaaacag caaaaagaac agatagcact
      781 ggcagggcag aatactgaac tggctaagat gaaatatcag gtcagtcagg gcgaattatc
      841 aaccctgtca gaagcgcaga aaaaaacgct tttacagaat gcagcactca tcgaccagaa
      901 aaagattcgt gagcagcttg ccgcgtatga aagcagtctg gcggacagta atgccagtgc
      961 ccgggcatct gacgacgcgc agttgctggg atatggtgaa ggctcacgga tgcgtgaacg
     1021 actccaggaa atgtggagta tccggcaggc gtttgagcag aaaaataacg agctgctgag
     1081 acagtatcag gccggagaaa ttgaagaagc cctgtggaaa caggagaaag aactgaataa
     1141 aaaatatctg gaagagcgtc tcagcgatca gcaggattat tatgcaaagg ccgatgcttt
     1201 acgtaataac tggaatgccg gactccagga gggactgacc aactgggcag acagtgccac
     1261 cgattatgct tcacaggcgg cagatgctgt cgtttccact atggacgggc tggtatcaaa
     1321 tatttccgat gcactggccg gaaatgttgt ggactggagg aactggggta gctcagttct
     1381 ccgggaagtt tcaaaaattt tgatgaatgc ggccattgtt aacggactga aatcactctc
     1441 tggtgccgga gggtggcttg gtacggtcgg cggatggatt tcgggggcgg tggcaaacgc
     1501 aaaaggtggt gtttacacat cggcaaatct gagtgcttac agtaacacta ttgtggatac
     1561 accgacgtat tttgcttttg cgaaaggtgc cgggctgatg ggtgaggccg ggcctgaagc
     1621 tatcatgcca ctgacacggg cagcggacgg ctctcttggg gtcagagcca ttggcaatgt
     1681 gaatggtggc agtggatttg tttattctcc cgtgtatcac atcagcattc agaatcaagg
     1741 gtgcaatggc gagatagatg cgcgctcagc caggggactg gtggatctga tcgacagcag
     1801 ggttgtgtca attatgcagt catcacgtcg ggacggagga ttatgcagtg cctgagtctg
     1861 aagtttttaa ctggatcccc cgcgagggga tggagacgac acgaaagcca tctgtgatta
     1921 cggtaaagtt cggtgacgga tatgaacagc gacgggcagg tggtctgaat gcagatctga
     1981 aaacgtttaa accggtattt cgggtcac
//
LOCUS       DAZFJL010000264         2007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFJL010000264 DAZFJL010000000
VERSION     DAZFJL010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.266"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..1854
                     /locus_tag="ACHJ8C_004667"
     CDS             <1..1854
                     /locus_tag="ACHJ8C_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5554363.1"
                     /translation="LPEASDNEEKTRQALKEQNRLIDEQKSKIKSLQEKIAGYQYVLA
                     NPGWTTDNGFMINHMTSVKTVTEGLAEATNQLAVEQSRLTQMQGKAQSIQDVLAGLEE
                     RRVALIRQQAAEQNKAYQSLLIMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQAT
                     LDDKQQTALNNSKRELALSRLKGEARERARLGYAADDLGFVGEAYQTARQNYINNSLD
                     AWRNNQANKPKAHKKTEAEKTEDIYKRLIKQQKEQIALAGQNTELAKMKYQVSQGELS
                     TLSEAQKKTLLQNAALIDQKKIREQLAAYESSLADSNASARASDDAQLLGYGEGSRMR
                     ERLQEMWSIRQAFEQKNNELLRQYQAGEIEEALWKQEKSLNEKYLEERLSDQQDYYAK
                     ADALRSDWNAGLQEGLTNWADSATDYASQAADAVVSTMDGLVSNISDALAGNVVDWRN
                     WGSSILQEVSKILMNAAIVNGLKSLSKSMSGAGGWLGTVGDWLSGAVANAKGGVYTSA
                     NLSAYSNTIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAIGNVNGGGGF
                     VYSPVYHISIQNKGSNGEIDTQSARGLVELIDSRVVSIMQSSRRDGGLCSA"
     gene            1847..>2007
                     /locus_tag="ACHJ8C_004668"
     CDS             1847..>2007
                     /locus_tag="ACHJ8C_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5554364.1"
                     /translation="MPESEVFNWIPREGMETTRKPSVITVKFGDGYEQRRAGGLNADL
                     KTFKPVFRVT"
BASE COUNT          573 a          429 c          585 g          420 t
ORIGIN      
        1 cttcctgaag cgtcagataa tgaggaaaag acgcgacagg cactgaagga gcagaacagg
       61 ttaattgacg agcagaaaag taagattaaa tccttacagg aaaaaattgc tggctatcag
      121 tatgtgctgg caaacccggg ctggacaacc gataacggtt ttatgattaa ccacatgacg
      181 tcggtaaaaa ctgttacaga agggcttgca gaagcaacaa atcaactggc agttgaacag
      241 tcccgtctca cacaaatgca gggcaaagcg caatccattc aggatgtgct tgccgggctg
      301 gaggagcgac gggtggcgtt gatccgtcaa caggccgcgg aacaaaacaa agcgtatcag
      361 tccctgttga tcatgaatgg gcagcatacc gagtttaatc gccttctcgg gctcggtaat
      421 gaattacttc agcagcgaca ggggctggtg aatgtaccgt tacggctacc acaggcaacc
      481 ctggatgata aacagcagac cgcactgaat aacagcaagc gcgaactggc tctgtcccgc
      541 cttaaggggg aagcgcgtga gcgtgcccga ctgggctatg ctgcggatga tctcggcttt
      601 gtgggagagg cgtatcagac agccagacag aattatatca ataactcact ggatgcctgg
      661 cgaaataacc aggcaaataa acccaaagcg cataaaaaga ccgaagcgga aaaaacagaa
      721 gatatttata aacggctgat taaacagcag aaagagcaga tagcactggc agggcagaat
      781 actgaactgg ctaagatgaa atatcaggtc agtcagggcg aattatcaac cctgtcagaa
      841 gcgcagaaaa aaacgctttt gcagaatgca gcactcatcg accagaaaaa gattcgtgag
      901 cagcttgctg cgtatgagag cagtctggcg gacagtaatg ccagtgcccg ggcatctgac
      961 gacgcgcagt tgctgggata tggtgaaggc tcacggatgc gtgaacgact ccaggaaatg
     1021 tggagtatcc ggcaggcgtt tgagcagaaa aataacgagt tgctgagaca gtatcaggcc
     1081 ggagaaattg aagaagccct gtggaaacag gaaaaatcgc ttaatgaaaa atatctggaa
     1141 gagcgtctca gcgatcagca ggattattat gcaaaggctg atgctttacg cagtgactgg
     1201 aatgccggac tccaggaggg gctgacgaac tgggcagaca gtgccaccga ttatgcttcg
     1261 caggcggcag atgctgtcgt ttccactatg gacgggctgg tatcaaatat ttccgatgca
     1321 ctggccggaa atgttgtgga ctggcgaaac tgggggagtt caattctcca ggaagtttca
     1381 aaaattctga tgaacgctgc catcgttaac gggctgaagt cactttccaa aagcatgtcc
     1441 ggtgccggag gatggcttgg tacggtcggc gactggcttt ccggtgcagt ggcaaacgca
     1501 aaaggtggtg tttatacatc ggcaaatctg agtgcttaca gtaacaccat tgtggatacc
     1561 ccgacgtatt ttgcttttgc gaaaggtgcc gggctgatgg gcgaggcagg gcctgaagct
     1621 atcatgccac tgacccgggc agcggacggc tctcttggag tcagagccat tggcaatgtg
     1681 aatggtggcg gtggatttgt ttattctccc gtgtatcaca tcagtattca gaataaaggg
     1741 agcaatggcg agatagatac gcagtcagcc agggggctgg tggaactgat cgacagcagg
     1801 gttgtgtcaa ttatgcagtc atcacgtcgg gacggaggat tatgcagtgc ctgagtctga
     1861 agtttttaac tggatccccc gcgaggggat ggagacgaca cgaaagccat ctgttattac
     1921 ggtaaagttc ggtgacggat atgaacagcg acgggctggt ggtctgaatg cagatctgaa
     1981 aacgtttaaa ccggtatttc gtgtcac
//
LOCUS       DAZFJL010000265         2001 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFJL010000265 DAZFJL010000000
VERSION     DAZFJL010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.267"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..125
                     /locus_tag="ACHJ8C_004669"
     CDS             <1..125
                     /locus_tag="ACHJ8C_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone CesF"
                     /protein_id="HFO5554365.1"
                     /translation="DDKILLNIALPKNKIDITEFTNLMENGYLLKKEIIKLLSL"
     gene            complement(326..835)
                     /locus_tag="ACHJ8C_004670"
     CDS             complement(326..835)
                     /locus_tag="ACHJ8C_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein EspH"
                     /protein_id="HFO5554366.1"
                     /translation="MSSSLSGITFTTFLTSNHASWNKLTRTLHISNDDILDELQLKAR
                     MQQRHHRVFAGIDDYSIINFRGKDYAVRFIKDNDNYVYKVNKITQESGCFSKIFSFLY
                     GGVTKALESKLNERHITPLSSTWFPRTALEGFLTERGLSSLLRRVQSTEGVNPPENTT
                     LNSLSGVTV"
     gene            complement(866..1783)
                     /gene="escQ"
                     /locus_tag="ACHJ8C_004671"
     CDS             complement(866..1783)
                     /gene="escQ"
                     /locus_tag="ACHJ8C_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HFO5554367.1"
                     /translation="MKPLSSQLNMKINDFYLPLLPVIGTGRLYITSEGHACHAYFREV
                     SGHGIRFTLTYSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDMIKLMILWVMQTAL
                     PEGDVSVDDVQFTMLNKDVYPVIENNNGENRLNVIILETTVQSLQHLINDNWQLVPHS
                     NALFFDGYIVPGWTDYPVTELRVGDSLRLYHVDDSQERRCWIVINNPLATVKLSDNNL
                     SVTDVLAADPLCTISSETVMNRIYCVIGTVHIDIHMLRNVQKDDIIDSTGYHLFGGCQ
                     LVRNNTTIAYGSIIKINEDFYFTISVVCD"
     gene            complement(1746..>2001)
                     /gene="escP"
                     /locus_tag="ACHJ8C_004672"
     CDS             complement(1746..>2001)
                     /gene="escP"
                     /locus_tag="ACHJ8C_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE needle length
                     regulator EscP"
                     /protein_id="HFO5554368.1"
                     /translation="LFYSSGRRRRYFEEGEHSFSILCGRLRGIVLTIKCSNGIVYLSI
                     KISPNNRNHVFLYHKKDYVFDKLKEIFPDEAIEFTIEYEN"
BASE COUNT          666 a          413 c          319 g          603 t
ORIGIN      
        1 aagacgataa aattttatta aacatcgcat taccaaaaaa taaaatagat ataacggaat
       61 tcactaactt gatggaaaac ggatatctac taaaaaaaga aataataaaa ctactttcac
      121 tttgatttat ttgcttttta ctttggaagt gataatatgg actggacggt ggcatccatt
      181 cagttcatat ttcacaaaaa ctacagtgct ctatccttga aatactaatc atttcgcctg
      241 catattcttt tcaaaaaata atgaaatatc gcccccagaa cagaacacat tctggggaga
      301 aaaagaaaac agcataaaat aatacttaaa ctgtcacacc tgataaagag tttaatgtag
      361 tattctctgg agggttcaca ccttctgtag actgtacgcg acgtaacaaa gaagagagcc
      421 ctctttcagt taaaaagcct tccagtgcag tgcgaggaaa ccaggttgaa gaaagtggcg
      481 taatgtgtct ctcatttaat ttactttcca gggccttcgt aacgcctcca tacaaaaagc
      541 taaatatctt tgagaaacat ccactttcct gagttatctt atttactttg tatacataat
      601 tatcattatc tttaatgaag cggacagcat aatcttttcc acgaaaattg ataattgaat
      661 agtcatcaat gcctgcaaaa acccggtggt gacgctgctg catgcgtgct ttaagttgca
      721 gttcatctag tatatcgtca ttcgatatat gaagagttcg ggtcaactta ttccatgacg
      781 catgattgct tgtcaaaaaa gtagtgaatg ttatacctga taatgatgac gacatcatac
      841 acctccctat ataacgcatt cctgattaat cacatactac gctaatagta aaataaaaat
      901 cttcgttgat ttttataata ctcccgtatg cgattgtggt attattccgt actaactggc
      961 acccaccaaa aagatgatat cccgttgaat cgataatgtc atctttttgg acattacgca
     1021 acatatgtat atcaatatgc actgtgccaa tcacacaata aatgcgattc atcacagtct
     1081 cgctgctgat agtacataac ggatctgcag ccagaacatc cgtgacagat aaattattat
     1141 cgctcagctt tacggttgcc aaagggttat taataacaat ccagcacctt ctctcttgac
     1201 tatcatcaac atgatagagt ctcagactat caccaactct caactctgta actggataat
     1261 cagtccaccc aggtacgata tagccatcaa aaaaaagagc attagaatgt ggaaccaatt
     1321 gccaattatc attaatcaga tgttgtaaac tttgtaccgt tgtttcgagt ataattacat
     1381 taagtctgtt ttctccatta ttattttcta ttaccggata aacatcttta tttagcatcg
     1441 taaattgaac atcatcaact gacacatcac cttccggtaa ggcagtctgc attacccata
     1501 agatcatgag ttttatcata tcttccggta tcagccggct ttcactatac gggaataact
     1561 cttggcacca ctgaatgaac tgttcttctg atatccagaa acgcccctca tagcccgagt
     1621 atgtcaacgt gaaacgaata ccatgaccgc tgacctcacg aaaatatgca tggcaagcat
     1681 gaccttcgga cgtaatatag aggcgaccgg ttccgataac tggcaacaac ggcaaataaa
     1741 aatcgttaat tttcatattc aattgtgaac tcaatggctt catcaggaaa gatttctttt
     1801 aacttatcga aaacatagtc ttttttatga tataaaaaaa catgatttct attatttggg
     1861 cttattttaa tagacagata aacgattccg ttactacatt taattgttaa aacaattcct
     1921 cgtaatcttc cacaaagaat actaaacgag tgctcccctt cttcaaagta gcgtcgtctt
     1981 cggcccgagg agtaaaacaa t
//
LOCUS       DAZFJL010000266         1951 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFJL010000266 DAZFJL010000000
VERSION     DAZFJL010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.268"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(115..333)
                     /gene="ymgF"
                     /locus_tag="ACHJ8C_004673"
     CDS             complement(115..333)
                     /gene="ymgF"
                     /locus_tag="ACHJ8C_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO5554369.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQH"
     gene            complement(465..1358)
                     /locus_tag="ACHJ8C_004674"
                     /pseudo
     CDS             complement(465..1358)
                     /locus_tag="ACHJ8C_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
     gene            complement(1656..1904)
                     /gene="ymgC"
                     /locus_tag="ACHJ8C_004675"
     CDS             complement(1656..1904)
                     /gene="ymgC"
                     /locus_tag="ACHJ8C_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO5554370.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIAQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT          619 a          424 c          385 g          523 t
ORIGIN      
        1 gtgataacac cagggttgac aactatttgt gcaataattc ctttccttca atgatccagg
       61 ggttcactgc cagcaaccca ttcagccgca tactggcagt gagaaactga aagattagtg
      121 ctgatccctg tggctgacga gtctttccct taatcgatta accagattga tcgccagcca
      181 aaccagggca ttaagcaaat ttatcgtcac aagcacttct cccagcccgg tgctgggttt
      241 tgcaataagg atgaccagga gagtgatcag cataaatgca atggaaaaga tgatatagag
      301 agtgaaataa tccagattat tactattgtt catgctacta atggcaatcc agtcaaaact
      361 ttcacacaat tatagcaaac aaaagaatga acaatagtaa caagaggcaa cgagctaatt
      421 agctgtctat aattacaccg caccatggcc ttgctgatca attttcactc aaccacaatc
      481 ttcaccttcg gtttagaaag gataattttg accaggtcga tgtatgtaac aggtttatag
      541 aaataataac cctgctgaaa tgtgatatta ttttggttca gatagtcaaa ttgttctttc
      601 gtttcgacat cttcagcgac gatactgatc gaaagtttac gcgccagatc caatacacaa
      661 tccagaattc gggttgatgc tgggtctgcg ttgacgcggc caacgaaact atgatcaatt
      721 ttgatataat caatatgcaa gtcatgcaga taagaaagtc ctgagtaacc agtaccgaaa
      781 tcatccagcg cgatgacaaa accattttca tgcagaatgt tcaaccgctg aaccagactt
      841 tcatcaacat ttaatggctc acgctcggtg acttccagaa caaggtttaa atcgcggcgg
      901 gtaaaactgt cacggtaatt taagcactcg tcgacaaacg tcggcgaaat aatatgcgag
      961 gcgctaaaat taatcccaat atgaaaacct tccggcagtt tactcgcgat agcgttcatc
     1021 tgtctggcaa cctgattcat caggctttgc gtaagcggaa cgattaatcc gcatttttca
     1081 gcaagtggaa taaatgccgc gggtgatata tatccaccgt gaggttgttt ccagcgggct
     1141 aacacctcaa ctccccgcaa tgtcccttcc cgaccattta ccacaggttg ataaaaaggg
     1201 acgatctccc cattatttat agcccgtcgc aggatctctt caggcgttgc gcttttattg
     1261 aaatacctat aaagcaggaa ggcagcggcg caggcgatta acagaataaa tatcaaaatc
     1321 ccaaagcctt tatcgataag tctgtagaag ctaaagagac gttcattcgc tcgcgctggg
     1381 gtctacagag agcatggtag agagcatcac aaatatccac gccgaagata aaattttctc
     1441 caacttcatc taacctatta attatttagc acaatgtagg cagaattaaa caaattaggc
     1501 gggatatcag gcgtcaagaa tggaaaacga gcgttctcca ttcctgtttg ccctctgata
     1561 ttgcggatat attaagaaag cataatgcct gaaatgctca ctttgcatca gcatagtgat
     1621 acagctgatg tttattcaaa aaccttactc aagttttaag agagcacgga ttccctgtca
     1681 ttaataatga tatccgttag tgtagtctta tgcatttcaa tgtactgcaa gatttgcaga
     1741 tagagatcag aaagaataac actggtctca tcttttaaac gcaagcgtaa agcctgagca
     1801 ataatatgac tatcaacata tccatgtgtc atgaacacct ccctctcgag atttttaaac
     1861 aaagcacatt gaaaaataaa tctctctggg attgaattat tcatgtcagc ctcgcaggaa
     1921 tagtgaccca ataaaattat gctgtgtata g
//
LOCUS       DAZFJL010000267         1940 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZFJL010000267 DAZFJL010000000
VERSION     DAZFJL010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.269"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            1309..1911
                     /gene="fimB"
                     /locus_tag="ACHJ8C_004676"
     CDS             1309..1911
                     /gene="fimB"
                     /locus_tag="ACHJ8C_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO5554371.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRVSEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEIQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          594 a          350 c          401 g          595 t
ORIGIN      
        1 ctttccacat tatttaaata aacagagaac cagattaaat acctgagtat aaaatctctt
       61 ctgatgttta attgatttga atgttcgtaa gctatatcac ttactcaatc cattttaccc
      121 agagtcataa aatagagtgt ctattgatct cattttcata ttcctttcag actgaagtgc
      181 gttttcatca cccgatgaga aagaactgaa gtgatgtttg ccatagattg cgagatgccc
      241 tgtctcattg actatcgcgg tgcaccttta tacctgttat accagatcaa aaatcacgca
      301 atccatacaa caaaacgaga tttgcaattc gtgtcacaaa atatgtcgat ctttttataa
      361 gaggaagatg ccatgtgaag ccagacgaac acttgcggtg gtcttaaaaa actaaatact
      421 ttcttttaac catttgtttt ataagtaatt tatttatagt ctaaacaagg gaagctttac
      481 aagctaactc agtgagcgtg gtgaaaataa gtgtttaccc gccatcaggc tgatcataat
      541 tctcatcatg aaatatgttt cctggttttt ggcttgtaag tggtcacccc tgaagtcgat
      601 ctggaaaggc ttgtagatgt tggtgttttc aggatgatgt ttcagatagg ttgtttgccg
      661 tatcgcccgg cgaatagctg tgattgagga aggtttaagt cgcagtgacc aaagccatat
      721 ttaccaacga atgtagatga aaaggcgtct cctgcgttcc cccatatctc caggaaatac
      781 acagtctgaa aaaggaatgt aacaatctca tggcgtaagc tgacgaacca ggcaggaata
      841 atcgctaggg acctaagcat tagcatgata atagccacta agaaattact gcgttccatg
      901 aaatagccat tttgtggcaa atgcagttga ctaataatgt catatgtgag acggctagtt
      961 aaacaaatat taaattttgc tgaatttttt atgttgattt tacttgttac agaacatatc
     1021 acatgatata tagataagat tagttgcatt aatgatgagg gttattattc gattcgtatc
     1081 agattgataa atatataaag gtacatagca tgcaagagca tggcgtttgt atggcaaagt
     1141 tattataagt aactatcgct actccattta agttcactca gaagaactgg tccacttacg
     1201 ttagttatta agcaaacgtt cgcttttata aacataatca ggattaaaat gttggattat
     1261 tgctaaccca gcacagctag tgcgcgtctg taattataag ggaaaacgat gaagaataag
     1321 gctgataaca aaaaaaggaa cttcctgacc catagtgaaa tagaatcact ccttaaagca
     1381 gcaaataccg ggcctcatgc tgcacgtaat tattgtctga cattactttg ttttattcat
     1441 ggtttccggg tgagtgaaat ttgccgattg aggatttcgg atattgatct taaggcaaag
     1501 tgtatatata tccatcgatt aaaaaaaggc ttttcaacaa cgcacccgct attgaacaaa
     1561 gaaattcagg ctttaaaaaa ctggttgagt atccgtactt cttatccgca tgctgagagc
     1621 gagtgggtat ttttatcacg taaggggaat ccactttctc ggcaacagtt ttaccatatt
     1681 atctcgactt ccggtggtaa tgccgggttg tcactggaga ttcatccgca catgttacgc
     1741 cattcgtgtg gttttgcttt ggcgaatatg ggaatagata cgcgacttat ccaggattat
     1801 cttgggcatc gcaatattcg tcatactgtc tggtataccg ccagcaatgc agggcgtttt
     1861 tacggcatct gggatagagc cagagggaga cagcgtcacg ctgttttata gagatagttg
     1921 agataccagg gatggtgtta
//
LOCUS       DAZFJL010000268         1915 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZFJL010000268 DAZFJL010000000
VERSION     DAZFJL010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.270"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..815)
                     /locus_tag="ACHJ8C_004677"
     CDS             complement(<1..815)
                     /locus_tag="ACHJ8C_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5554372.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQHLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLIEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWA"
     gene            972..1892
                     /locus_tag="ACHJ8C_004678"
     CDS             972..1892
                     /locus_tag="ACHJ8C_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5554373.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTTLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPEAALEAGLTEIDLFEATEYYRSPRGCAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPLMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
BASE COUNT          544 a          430 c          421 g          520 t
ORIGIN      
        1 gcccaatcct caagaatcgg ctgtaacttg cccgcattga tatgttcagc gattgcaaaa
       61 tccagatgtt gaatgatacc cgttccattc agcgccattc gtatcatgct ttcgtcatca
      121 ttagaaatat acttacctga cacctctatt tcgaactctt cattaccccc cacaacattc
      181 gaaaactccc atgctgagag tgcaccgcta cttttatgcc gcaaccgaag acaattatgg
      241 ttactcaatt cctgaggagt ttcaggaata ccgtgttctt ttaaataatc tggcgatgcc
      301 acagtaacca gtttaataag cggagaaaca ggaacggcag tcatatgctc atccagtcct
      361 tgctcaagac gtatgcccac atcacagccg tccgcaataa tgttagataa accgtcatcc
      421 ataatgagtt ctatctttgt tttggggtaa cgagtaagaa actcgccaat atgaggctca
      481 ataagcatcc ttgctgcaac acgggaggaa tttattctca acagtcctgt aggttcaagt
      541 tgggtgtcac cgacatcttt caacgcatca tcaatttgcc ccaaagccga caccagcact
      601 tcatacagat gctgtccttc ctcagtaagc gacatattgc gggtcgtacg gtatatcagc
      661 ttggtgttaa gacgttcctc aagagatttt aaattctggg acaatgcggc ccgcgtaata
      721 cccatttcag aggctgctct ggaaaaactg ccggcacgaa caatcagcgc aaaccaggca
      781 agagaaggca aaattgaagg ctctattttc atcattgtaa accttaactt aacactgtga
      841 tagatattag accattgatg ataaccaata caagtcatac tattacgccg ttcgcgatat
      901 gaacacgaat gccagttgcg gataatagaa atgatattta aatctgttta ggcaaatccc
      961 tggagcttga aatgaaaact gtttcgataa aacacactta ctgggatata gctgctgata
     1021 tctatttccc tgacgatttt aatagtgaaa aaaagtatcc tgccattatc agtgcccatc
     1081 caattggaag ctgcaaagaa cagacatcgg gtagtgtgta tggtgccgcc ctggcaaaag
     1141 ctggctttat cgtcattgct tttgatgcca gttttcaggg aagcagcggt ggagaaccgc
     1201 gttatctgga agatccaaca atgcgagtaa aagacttcag cattgtcgtt gattacctta
     1261 ccaccttacc ttacgttgat gccgggcgta ttggtgtact gggtatttgt ggtggaggtg
     1321 gatatgccat taatgccgca atgacagaac gtcggattaa agccattggt actgtcacgg
     1381 gtgcaaacta cgggcgtctt atgcgtgagg gatttacagc gtttaatcct atcggagcac
     1441 tcgaagcaat ggcgcaacaa agaacagacg aagctaacgg cgcaaaactt cgcgtagatg
     1501 atttgctccc ttcttctccg gaagcagctc ttgaagcggg attaacagaa atagaccttt
     1561 ttgaagcaac agaatattat cgttcgccac gaggctgtgc gcctaatggg gtaaatcgtt
     1621 cgctattttc acatcagact gttgccgtag gctgggatgc ttttcatctt gcagaagtcc
     1681 tgttaactca accactaatg gttgtagtgg gcgatagagt gggcgctttt ggtgcctatc
     1741 gtgacggctg cgaaattatt ggtcgtgcag cctcaaaaca taaagagctg gtcgtggttg
     1801 aaggttattc acattacgac ctgtatgata agccagaacc tgtgaaacag gcgctcgata
     1861 aattaattcc tttttacaaa acacatttgt aataaccttg cagggagatt catta
//
LOCUS       DAZFJL010000269         1884 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFJL010000269 DAZFJL010000000
VERSION     DAZFJL010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.271"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            67..321
                     /gene="bssS"
                     /locus_tag="ACHJ8C_004679"
     CDS             67..321
                     /gene="bssS"
                     /locus_tag="ACHJ8C_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO5554374.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            436..1554
                     /gene="solA"
                     /locus_tag="ACHJ8C_004680"
     CDS             436..1554
                     /gene="solA"
                     /locus_tag="ACHJ8C_004680"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO5554375.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDDLSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGDYQAKKAIVCAGTWVKDLLPELPVQPIRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKTGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1602..1715
                     /locus_tag="ACHJ8C_004681"
     CDS             1602..1715
                     /locus_tag="ACHJ8C_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO5554376.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          495 a          461 c          437 g          491 t
ORIGIN      
        1 tgcttcagat aaaaacgcat tgaacctcga ataacgttgt ctagtaacac gaattagggg
       61 gccatgatgg aaaaaaataa tgaagtcatt cagactcatc cgctcgtagg gtgggacatc
      121 agcaccgttg atagctatga tgcgctgatg ttgcgtttgc actaccagac cccaaataag
      181 tccgagcagg aagggactga agttggtcag acgctctggt taaccactga tgttgccaga
      241 caatttattt cgatattaga agcaggaatc gccaaaattg aatccggtga tttcccggta
      301 aacgagtatc ggcgtcatta acatgacatg accatccacc caaaaggcac ctcaccggtg
      361 cctttaccat ttctgggttg tatatctgca ttcagttaat tactctgctt aaaccatgag
      421 agcagagaga aaaaaatgaa atacgatctc atcattattg gcagcggttc cgtaggcgct
      481 gccgccgggt attatgcaac ccgcgccggt ttaaacgtgc taatgaccga cgcccatatg
      541 ccaccacatc aacacggcag ccaccacggc gatacgcgat taattcgcca tgcttatggt
      601 gaaggcgaaa agtatgtccc gctggtcctc cgcgcgcaaa cactgtggga tgacctctcc
      661 cgccacaacg aagatgatcc catttttgta cgctctggtg tcattaacct tggcccggct
      721 gactccgcat ttctcgccaa cgtcgcccac agcgccgaac agtggcaact caatgttgaa
      781 aagctcgatg cgcaagggat tatggcccgc tggccagaaa tacgcgtccc ggacaactac
      841 atcggcttat ttgagactga ttccggcttt ttacgcagcg aactggcgat taaaacctgg
      901 atccaactgg cgaaggaagc gggctgtgcg cagctgttca actgcccggt caccgcaatt
      961 cgtcatgacg atgatggcgt aactattgaa acggctgacg gtgattatca ggcgaaaaaa
     1021 gcgattgtct gcgcgggaac atgggtaaaa gacctgctcc cggagctgcc tgtccagccc
     1081 atacgcaaag tatttgcctg gtatcaggcc gatggtcgct atagcgtgaa gaataaattc
     1141 ccggcgttta ccggtgaact gcccaatggc gatcaatatt atggttttcc ggcagaaaac
     1201 gacgcgttga agactggcaa acataacgga ggccaggtta tccattcagc ggatgaacgt
     1261 gttccgtttg cggaagtggt cagcgatggt tcggaagcct tcccgttctt gcgcaatgtc
     1321 ttgccgggta tcggttgctg cctgtacggc gctgcctgca cctatgataa ttcgcctgac
     1381 gaagatttta ttatcgatac cctacccggc cacgataata cactgctcat taccggcctg
     1441 agtgggcacg gttttaaatt tgcgtcagtt ttaggggaaa tagctgccga ttttgcgcaa
     1501 gacaaaaaaa gcgattttga tttgacgcca ttcaggcttt cccgcttcca ataatcatca
     1561 ttacggcctc cagaatgagg ccgtttttac aggagtattt aatgcgtcgg ctgttgcact
     1621 atctcatcaa taatattcgc gagcatctga tgctttatct ttttctctgg ggactgttgg
     1681 caattatgga cctgatctac gtgttttatt tttagttttt ccgtactgac ataaactgac
     1741 aaatatttaa tctccctttc atttagcgtg aaaaattgaa tgaaattctt cctgcctcta
     1801 cagcactcac cagaccttcc aaatgctgaa tccactaaac ttatgaagat tctttcatta
     1861 ttaattcaaa attattgaaa taaa
//
LOCUS       DAZFJL010000270         1867 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZFJL010000270 DAZFJL010000000
VERSION     DAZFJL010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.272"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            125..1768
                     /gene="yojI"
                     /locus_tag="ACHJ8C_004682"
     CDS             125..1768
                     /gene="yojI"
                     /locus_tag="ACHJ8C_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO5554377.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQNGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT          424 a          448 c          518 g          477 t
ORIGIN      
        1 cgactgccgc tacaacctga cattccgtca ggcaggtaaa aaagaataaa aataagaatt
       61 attattgctg tgcgcacgaa gatgtttaaa ctgcgggctg taattcattg tccgggtttt
      121 ctgcatggaa cttcttgtac ttgtctggcg gcagtatcgc tggccattta tcagtgtgat
      181 ggcgctaagc ctcgccagtg cggcattagg cattggctta attgctttta tcaatcagcg
      241 ccttatcgaa acggcggata ccagtctgct ggtgttgccg gagtttctgg gattattgct
      301 gctgttgatg gcagtcactc tcggatcgca actggcgctc accactttgg ggcatcactt
      361 cgtttaccga ctgcgtagtg aatttatcaa gcggattctg gatacccatg tcgagcgcat
      421 tgaacaactt ggtagcgcct cgttgctggc ggggttaacc agcgatgtgc gcaatatcac
      481 cattgctttt gtgcgtctgc cggaactggt gcaggggatc attctcacta tcggttcagc
      541 ggcgtatttg tggatgctgt cgggcaaaat gttgctggta acggctatct ggatggcagt
      601 caccatctgg ggcggttttg tgctggtggc gcgggtgtac aaacatatgg cgaccctgcg
      661 tgaaaccgaa gataagctgt acacggattt tcaaactgta ctcgaagggc gcaaagagct
      721 gactctgaac cgtgaacgcg ccgagtatgt gtttaacaac ctctacattc ctgatgcgca
      781 agagtatcgc catcatatta tccgcgcaga caccttccat cttagtgccg tgaactggtc
      841 aaacatcatg atgctgggcg caatcggcct ggtgttctgg atggcgaaca gtctcggttg
      901 ggctgatacc aacgttgccg cgacctattc gttgacgctg ttattcctgc gtacgccgct
      961 gctttcggcg gttggcgcat taccgacgct gctgacggcg caggtggcgt ttaacaagct
     1021 gaacaaattc gcgctcgcgc ctttcaaagc agagtttccg cgcccgcagg cgtttcccaa
     1081 ctggcaaacg ctggagctgc gtaacgtgac gtttagctat caggataacg cgttttccgt
     1141 tgggccgatt aacctcacca ttaaacgtgg cgagctgctg tttctgattg gtggcaacgg
     1201 tagtggaaaa tcgacgctgg cgatgttgct gacgggcttg taccagccac aaaatggcga
     1261 aatcttgctg gatggcaaac ctgtcagcgg cgaacaaccg gaagattatc gaaaactgtt
     1321 ttcggcagtg tttaccgatg tctggctgtt tgatcaactg ctggggccgg aggggcaacc
     1381 cgctaacccg caactggttg agaagtggct ggcgcagctg aaaatggctc ataagcttga
     1441 gttaagcaac gggcgtattg ttaacctgaa gttatcaaaa gggcagaaaa aacgcgtggc
     1501 gctgttgctg gcgctggcag aagaacgcga tattatcctg ctggatgaat gggcggcgga
     1561 tcaggatcca cacttccgtc gtgagtttta tcaggtgctg ctgccgctga tgcaggagat
     1621 gggtaaaact attttcgcca tcagtcatga tgatcattac tttatccacg ccgaccgcct
     1681 gctggaaatg cgcaacgggc aacttagcga gctgacaggc gaagagcgcg atgctgcttc
     1741 gcgtgatgcc gttgcccgga cggcataata ttacgacgct gacctggcgg actgctgccg
     1801 ccaggtcaat attctaaaaa taccttcgtt tctaataacc ctataattaa tcaacgaaat
     1861 tataatg
//
LOCUS       DAZFJL010000271         1783 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFJL010000271 DAZFJL010000000
VERSION     DAZFJL010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.274"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            89..748
                     /locus_tag="ACHJ8C_004683"
                     /pseudo
     CDS             89..748
                     /locus_tag="ACHJ8C_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_046881606.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(898..963)
                     /locus_tag="ACHJ8C_004684"
     CDS             complement(898..963)
                     /locus_tag="ACHJ8C_004684"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAST1 family heat-stable enterotoxin"
                     /protein_id="HFO5554378.1"
                     /translation="MWCVTACALCHGRTTKPSPAT"
     gene            complement(935..1141)
                     /locus_tag="ACHJ8C_004685"
                     /pseudo
     CDS             complement(935..1141)
                     /locus_tag="ACHJ8C_004685"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1433..>1783)
                     /locus_tag="ACHJ8C_004686"
     CDS             complement(1433..>1783)
                     /locus_tag="ACHJ8C_004686"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554379.1"
                     /translation="EAAIVLSLATAVVSGVLYFYNSAKENNAPPETTKGLQSLIGATQ
                     ALYGSRGQTAGTTLSAQAVSAISGIEIGSPKIAKGEQNVFYIPGRWQKQPFSLTIIRQ
                     YLRYIQHQRIYVWL"
BASE COUNT          451 a          400 c          403 g          529 t
ORIGIN      
        1 gggctcagag gaatccctag aaaacaagac aggtatagtg agatgtgatc taattgatta
       61 tgctaataat ttcaaggaga ttaccactat gcctgcacgt tcatcatgcc aaaatttcct
      121 taacaacata ctggctcctt tacgtcttta ccgtcaaaaa tcactgattg acgcaactaa
      181 tgctgtaata aatgccgctt ctctcacgct taccagtatc gagcatcatt taaccgatac
      241 ggcatcagtt aaaaacaaaa taaagcgagt ggatcgactg ttggggagtc gacatttgca
      301 aaatgaggtt tccaccattt ttcagcgaat tacccagaag attatgcagg gaatgtctcg
      361 tgttgtgata ctcattgact ggagtgctta tcatgcctct cgttttcagc ttttgcgggc
      421 aagcttagca tgtgacgggc gttctttacc gttgatgagt tgtgtcgttc catcatcgca
      481 gacaacgaat gctgacgtac atgagcattt tctggaatcg cttgctgagt gcttttccac
      541 cgggactaat gtcattgtta ttacagatgc cggttttcag gtgcgatggc tccaacagct
      601 ccgctcccgt ggctggagct atatttgccg ggtactgggc aatgattatt ataatgttgg
      661 tgatggctgg gaaaaggtgt cggactcagg cacaaaagca tcaacgacag caatttattt
      721 aggtgaatgc ctgctgggtc gagataaatg tccggcgaat aagcgaagaa cgtggcaaga
      781 ttcggcctgt tagcctgcca gcttcggctt atctgaggat agcgactgtc ccaggccgcc
      841 gcgaacgctt tcagtgcctg ccggcctgcc tcttccgggg agcctgataa attgctttca
      901 ggtcgcgggt gacggctttg ttgtccttcc atgacacaaa gcgcaggctg ttacgcacca
      961 catgcacgat gcataaccgg atgcgggcct ccggatatat tgtgttgatg gcatccggga
     1021 agcctttcag gccatccaca catgcgatga ggatatcgtt cagaccgcgg tttttcagtt
     1081 cagtcagggc attgagccag aacttcgccc cttcattttc ggccagccac atccctggca
     1141 tttgccttga gaaacttgaa atgtgagtta aggtttgtcg ctgtctgtca gtgcagggac
     1201 aaacctctat gtttctattt atttacactt ttgaaagttc aacattactg aacttttaac
     1261 tcatacatta tatgtgctaa atttgtgttt gtagtttcac cacatatcga tgcagcatca
     1321 tttggagtac ttacagatat tgcggagtta taatcagaac tatatatttg tctaccataa
     1381 ctaagactta ccctgggttt tcctgtagtc attgttccca aattaacgcc ggctaaagcc
     1441 atacatatat cctttgatgc tgtatgtacc gtaaatactg tcttattatt gttaaggaaa
     1501 aaggttgctt ctgccatctc cccggaatat agaaaacatt ttgttcacct ttggctattt
     1561 tcgggctgcc tatttctata ccagaaatag cggaaacggc ttgcgcacta agagttgttc
     1621 cagctgtctg tcctctgcta ccgtataacg cttgagtagc cccaatcagg gattggagtc
     1681 cctttgtagt ttctggcgga gcattatttt cttttgctga attatagaaa tataaaacac
     1741 cagaaactac cgctgtagct aaagatagca cgattgcagc ttc
//
LOCUS       DAZFJL010000272         1781 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFJL010000272 DAZFJL010000000
VERSION     DAZFJL010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.275"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            24..265
                     /locus_tag="ACHJ8C_004687"
                     /pseudo
     CDS             24..265
                     /locus_tag="ACHJ8C_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(346..504)
                     /locus_tag="ACHJ8C_004688"
     CDS             complement(346..504)
                     /locus_tag="ACHJ8C_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO5554380.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
     gene            complement(590..1333)
                     /locus_tag="ACHJ8C_004689"
     CDS             complement(590..1333)
                     /locus_tag="ACHJ8C_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554381.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFWLFSFLTGYYKPQFKKEYEMINYNDGWYYVLARYDNCLVLST
                     SFNAGSKRFVIYQSAQDKNLQVDIVRTRI"
     gene            complement(1586..>1781)
                     /locus_tag="ACHJ8C_004690"
     CDS             complement(1586..>1781)
                     /locus_tag="ACHJ8C_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO5554382.1"
                     /translation="CGRCKGVGYSRMPASAAYRAVTMLIPNLTQPTWSRTVKPLYDAL
                     VVQCHKEESIADNILNAITR"
BASE COUNT          628 a          417 c          305 g          431 t
ORIGIN      
        1 tctgacggcg acgccgccct gacaacatcg taatgtttaa tcgacatcga atttctcccg
       61 tgtagaggaa cagagttaaa aagccggaag cggaatcaaa tcacaggatg accatctgcc
      121 agtggcaggt cataaaaaaa aggctgcgca atgcgcagcc agaactcaca aggaaaatga
      181 taaaaggaat aacactagtg atgtacgcat ggcgcctccc gctaagttct gcaatgatca
      241 aacagaactc gctacgtgcc cttaaaactc gatcatttag cccctccaag gaggattcac
      301 catgcggttg attttttaat aaacagtaaa caaaaaagtc aagaattatt cattctgttc
      361 tttcatcatc ggccacagca ataccacaat gccgcagacc agagcgccat cagtcagtac
      421 caacattatc ctgctggtga aatccatcat caccatcact aaaagcagga tcacaacagc
      481 aagcagacac agtttataaa acaatgttca gaaaacgcat tcagcatgcc taaggttcta
      541 ttcctacgaa tagccaactt gcaacttaaa atattattta tgcagccaat taaattctgg
      601 tccttacaat atcaacctga agattcttat cttgtgctga ttgataaatg acaaaccttt
      661 tactacctgc attgaaagaa gtagacaaaa ccagacaatt atcataacga gcaagaacat
      721 aataccaacc atcattataa ttaatcattt catattcttt cttaaactgc ggtttgtaat
      781 atcctgtcag aaatgaaaaa agccagaaat atgccacaaa agcaatcatc acaatctcaa
      841 aaaaatgttt ttttataaat ggcttatcat agaagcatga taccgataaa aatcgcccat
      901 aagatcttat cgaaattgta accgccagcg caatcgctgc tgacagtagc aaaagaggta
      961 cctgaatctt ctgtctcaat atagaaaact caataattgc cggcacaaac aataattcca
     1021 cagcaaaata aaggcgaaat acatttagct cttgcataga atgttttctt ttcactgcga
     1081 aaaagaatac aacaccaata ccccaaccga taagaaatat agcaatgacg ataactgcaa
     1141 aaaataaact tctggcaaca tcatcaacac ctgcacctac aatccaccat gggaagccgt
     1201 agtaaaaaga agtaccccat ccatagaaat aagcactccc ccatccaagg catcccatgt
     1261 aggcaataaa aagtgaagaa ctcctgagca gcgcaccatc cttcataacc accccaatac
     1321 aagatgataa cattggctta caactcataa caaaagcaat tcaatgccgt caagaggtta
     1381 caggctaaaa aaactctatt acattgcagt cagcatgttt actacacaaa tacaattcag
     1441 agcataaaaa ctactcggcg gcaggttatt gagactcatc aatgacatgt aaaaaacgcc
     1501 cattattggt gtcaagtttc cccaaagtta ttcaaaaagt caatattatg ccgttaatat
     1561 gttgccatcc gtggcaatca tggcgctaac gtgtgatcgc attcaaaatg ttgtctgcga
     1621 ttgactcttc cttgtggcat tgcacaacca gagcgtcata cagcggctta acagtgcgtg
     1681 accaggtggg ttgggtaagg tttgggatta gcatcgtcac agcgcgatat gcggcgcttg
     1741 ctggcattct tgaataaccg acgcctttac atcttccgca c
//
LOCUS       DAZFJL010000273         1767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFJL010000273 DAZFJL010000000
VERSION     DAZFJL010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.276"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            49..540
                     /locus_tag="ACHJ8C_004691"
     CDS             49..540
                     /locus_tag="ACHJ8C_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5554383.1"
                     /translation="MGKKTAPLLPYTNQMLSEFGERLKLARLRRRLTAKQVAERAGMS
                     PITLRSLESGSAGVTIGAYLSVMQVLGLETDLNKLAAEDDLGRQLQDSRLIKKRGLSR
                     SSGKNEEGIQTDPFQTRYEKEGLLTTDLTALAEGSTPSITGETSTSLAALLKPIKKPL
                     KGN"
     gene            543..>1767
                     /locus_tag="ACHJ8C_004692"
     CDS             543..>1767
                     /locus_tag="ACHJ8C_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HFO5554384.1"
                     /translation="MALIGVYADWEGLDGPERIGYLHSRRTRTREIFEFEYDKKALAD
                     PSLNFIQLDPEIMLYEGAQYPIPPKDKFGAFSDSCPDRWGRMLMKRRFERDIRDGLCD
                     KDSHLYESDYLLGVHDLYRVGALRYKREDAGEFLDNRIDVAAPPFTEIASLERASRAI
                     EEDPDNKELMGQKWLRMLIAPGGSLGGARPKASVVDEAGHLYIAKFPSVKDEYDVGGW
                     EMVVNALAVGCGLNVAPAQAHKFASNYHCFMVRRFDRTNAGRRLHFASAMTLTRHQDG
                     EDASTGVSYLELADVLIRHGAQTNTDLKELWSRIVFNILVSNTDDHLRNHGYILIPGK
                     GWRLSEAYDLNPVARSDGLKLNITENDNALDLELAREVAEYFRLGLTEADDIIANFRG
                     IVSQWRIIAERLRLPG"
BASE COUNT          492 a          325 c          452 g          498 t
ORIGIN      
        1 gctaatatta tggctatcat tgccataaaa agataaaata ttatcactat gggtaaaaaa
       61 acagcaccgt tactccctta tacgaatcag atgcttagtg aatttggtga gcggctgaaa
      121 cttgctcgtt tacgacgcag gcttaccgca aagcaagttg ctgaacgtgc tgggatgtcg
      181 ccaataacat tgcgctctct ggagtcagga agtgcagggg tcacgatagg ggcatatttg
      241 tctgtgatgc aggttttggg cctggaaacg gatttaaata agcttgcagc tgaagacgac
      301 ttagggcgtc agctccagga ttcccgtttg attaaaaaaa gagggctttc tcgttcatca
      361 ggtaaaaatg aagaaggtat tcagacagac ccttttcaaa cgaggtatga gaaagaaggt
      421 ttactcacca cggatttaac agcactggca gaaggctcaa cgccatctat tacaggagaa
      481 acatcaacct ctcttgcagc actattaaaa ccgataaaaa aaccgttaaa aggaaattaa
      541 ttatggcatt aatcggagtg tatgcggatt gggaaggcct ggatggtccg gagcggattg
      601 gttatttaca cagtcgccgc actcggacgc gtgagatatt tgagtttgag tacgataaaa
      661 aggcgcttgc agatccttca cttaatttca ttcagttaga tcctgaaata atgttgtatg
      721 agggagcaca gtacccaatt cctccaaagg ataaatttgg tgcatttagt gattcctgcc
      781 cggatcgttg ggggcgtatg ctaatgaagc gtcgttttga gagggatatt cgagacgggt
      841 tatgtgataa agactcccac ctttatgagt ctgattatct actaggtgtt catgatcttt
      901 atcgggttgg ggctcttcga tacaaacgtg aagacgctgg agagtttctt gataatcgca
      961 ttgatgttgc agccccacca tttaccgaaa tagcgagtct ggaaagggct agccgggcaa
     1021 ttgaagaaga tcctgataat aaagagctaa tggggcaaaa atggttaaga atgctaatcg
     1081 ctccgggggg atcattaggg ggggcacgtc cgaaagccag tgttgttgat gaagctgggc
     1141 atctctacat cgctaaattt cccagtgtaa aagatgagta tgatgttggt ggatgggaaa
     1201 tggtcgttaa tgcacttgct gttggctgtg gactgaatgt tgcacctgct caggcgcata
     1261 aatttgccag caattaccat tgttttatgg ttcgtcgttt tgaccgcact aatgcgggaa
     1321 ggcgtctgca ttttgcttcg gctatgacac taaccaggca tcaggatggt gaagatgcct
     1381 ctacaggtgt gagttatctg gaattagcag atgttcttat cagacatgga gcgcagacta
     1441 atactgacct taaggaactc tggtccagga ttgtttttaa tattcttgtt tctaataccg
     1501 atgaccattt acgtaatcat ggatatatct tgataccggg aaaaggttgg cggctttctg
     1561 aggcatatga tttgaatcct gttgccaggt ctgacggtct aaaactcaat attacagaaa
     1621 atgataatgc acttgatttg gaattagctc gagaagttgc ggagtatttt cgacttggtt
     1681 tgacagaagc agatgacatt attgctaatt ttagagggat tgtcagtcag tggcgaatca
     1741 ttgccgagcg tcttcgatta cctggtc
//
LOCUS       DAZFJL010000274         1739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFJL010000274 DAZFJL010000000
VERSION     DAZFJL010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.277"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            33..>1739
                     /gene="tir"
                     /locus_tag="ACHJ8C_004693"
     CDS             33..>1739
                     /gene="tir"
                     /locus_tag="ACHJ8C_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFO5554385.1"
                     /translation="MPIGNLGHNPNVNNSIPPAPPLPSQTDGAGGRGQLINSTGPLGS
                     RALFTPVRNSMADSGDNRASDVPGLPVNPMRLAASEITLNDGFEVLHDHGPLDTLNRQ
                     IGSSVFRVETQEDGKHIAVGQRNGVETSVVLSDQEYARLQSIDPEGKDKFVFTGGRGG
                     AGHAMVTVASDITEARQRILELLEPKGTGESKGAGESKGAGESKGAGESKGVGELRES
                     NSGAENTTETQTSTSTSTSTSTSSLRSDPKLWLALGTVATGLIGLAATGIAQALALTP
                     EPDSPTTTDPDAAASATETATRDQLTKEAFQNPDNQKVNIDELGNAIPSGVLKDDVVA
                     NIEEQAKAAGEEAKQQAIENNAQAQKKYDEQQAKRQEELKVSSGAGYGLSGALILGGG
                     IGVAVTAALHRKNQPVEQTTTTTTTTTSARTVENKPANNTPAQGNVDTPGSGDTMESR
                     RSSMASTSSTFSDTSSIGTVQNPYAEVKTSLHDSQVPTSNSNTSVQNMGNTDSVVYST
                     IQHPPRDTTDNSARLLGNPGAGIQSTYARLALSGGLRHDMGGLTGGSNSAVNTSNNPP
                     ALG"
BASE COUNT          482 a          379 c          469 g          409 t
ORIGIN      
        1 atctatttac ataaaataaa aaggagatat ttatgcctat tggtaatctt ggtcataatc
       61 ccaatgtgaa taattcaatt cctcctgcac ctccattgcc ttcacaaacc gacggtgcag
      121 gggggcgtgg tcagctcatt aactctacgg ggccgttggg atctcgtgcg ctatttacgc
      181 ctgtaaggaa ttctatggct gattctggcg acaatcgtgc cagtgatgtt cctggacttc
      241 ctgtaaatcc gatgcgcctg gcggcgtctg agataacact gaatgatgga tttgaagttc
      301 ttcatgatca tggtccgctc gatactctta acaggcagat tggctcttcg gtatttcgag
      361 ttgaaactca ggaagatggt aaacatattg ctgtcggtca gaggaatggt gttgagacct
      421 ctgttgtttt aagtgatcaa gagtacgctc gcttgcagtc cattgatcct gaaggtaaag
      481 acaaatttgt atttactgga ggccgtggtg gtgctgggca tgctatggtc accgttgctt
      541 cagatatcac ggaagctcgc caaaggatac tggagctgtt agagcccaaa gggaccgggg
      601 agtccaaagg tgctggggag tccaaaggtg ctggggagtc caaaggtgct ggggagtcaa
      661 aaggcgttgg ggagttgagg gagtcaaata gcggtgcgga aaataccaca gaaactcaga
      721 cctcaacctc aacttcaact tcaacctcaa cttccagcct tcgttcagat cctaaacttt
      781 ggttggcgtt ggggactgtt gctacaggtc tgatagggtt ggcggcgacg ggtattgcac
      841 aggcgcttgc attgacgccg gagccggata gcccaaccac gaccgaccct gatgcagctg
      901 caagtgcaac tgaaactgcg acaagagatc agttaacgaa agaagcgttc cagaacccag
      961 ataatcaaaa agttaatatc gatgagctcg gaaatgcgat tccgtcaggg gtattgaaag
     1021 atgatgttgt tgcgaatata gaagagcagg ctaaagcagc aggcgaagag gccaaacagc
     1081 aagccattga aaataatgct caggcgcaaa aaaaatatga tgaacaacaa gctaaacgcc
     1141 aggaggagct gaaagtttca tcgggggctg gctacggtct tagtggcgca ttgattcttg
     1201 gtgggggaat tggtgttgcc gtcaccgctg cgcttcatcg aaaaaatcag ccggtagaac
     1261 aaacaacaac aactactact acaactacaa gcgcacgtac ggtagagaat aagcctgcaa
     1321 ataatacacc tgcacagggc aatgtagata cccctgggtc aggagatacc atggagagca
     1381 gacgtagctc gatggctagc acctcgtcga ctttctctga cacttccagc atagggaccg
     1441 tgcagaatcc gtatgctgag gttaaaacat cgctgcatga ttcgcaggtg ccgacttcta
     1501 attctaatac gtctgttcag aatatgggga atacagattc tgttgtatat agcaccattc
     1561 aacatcctcc ccgggatact actgataaca gcgcacggtt attaggaaat ccaggtgcgg
     1621 ggattcaaag cacttatgcg cgtctggcgc taagtggtgg attacgccat gacatgggag
     1681 gattaacggg ggggagtaat agcgctgtga atacttcgaa taacccacca gcgctggga
//
LOCUS       DAZFJL010000275         1736 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFJL010000275 DAZFJL010000000
VERSION     DAZFJL010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.278"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(76..1482)
                     /gene="gndA"
                     /locus_tag="ACHJ8C_004694"
     CDS             complement(76..1482)
                     /gene="gndA"
                     /locus_tag="ACHJ8C_004694"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO5554386.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNKELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDDDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKGEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFSAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT          453 a          425 c          393 g          465 t
ORIGIN      
        1 tctataagat atttggcaaa aaaaagcccg gtgcaatata cgccgggcct caattttatt
       61 gttggttaaa tcagattaat ccagccattc ggtatggaac acaccttctt tatcaatacg
      121 cttataagta tgcgcaccaa aatagtcacg ctgtgcctgg atcaggttcg caggcagaac
      181 agcagcacgg tagctgtcgt aataggcaac cgctgcggag aaggtcggaa ccggaatacc
      241 gttctgtact gcataagcaa cgacatcacg cagcgcctgc tggtagtcat cggcaatttg
      301 cttgaagtac ggagccagca gcaggttagc gatctgcgga ttttcggcat aagcatcggt
      361 gattttctgc aagaactgcg cacggatgat gcagccagca cggaaaatct tcgcgatttc
      421 gccgtaattc agatcccagt tgtactcttc agacgccgca cgcagctgag agaagccctg
      481 agcgtaagaa acgattttgc ccagatacag agcacgacga actttttcga tgaactcacc
      541 tttgtcgcca gctggctgcg cttgcgggcc agagagaact ttagatgcgg caacacgctg
      601 ctctttcaga gaagagatat aacgtgcaaa cacagactcg gtaatcagcg acagcggttc
      661 tccgagatcc agcgcactct ggctggtcca tttacccgta cctttgtttg ctgcttcatc
      721 cagaatcaca tcaaccaggt agttaccgtc atcatctttt ttggtgaaga tatctttggt
      781 gatatcgatc aggtagctgc tcagttcacc gttattccac tcggtaaagg tctgcgccag
      841 ttctttgttg gtgaggttca ggccaccttt aagcagagaa taggcttcag caatcagctg
      901 catatcgccg tattcaatac cgttgtgaac catcttcaca tagtgacctg cgccatcggc
      961 accaatatat gtaacgcacg gttcgccatc ttcagcaacg gcggcgattt tggtcaggat
     1021 cggcgcaacc agctcatagg cttctttctg gccaccaggc ataatggaag gacctttcag
     1081 cgcaccttct tcaccaccgg agacaccggt acctataaag ttaaaacctt ctgcagaaag
     1141 ctcgcggtta cgacggatgg tatcctggaa gaaggtgtta ccaccatcaa tgatgatgtc
     1201 acctttatcg aggtacggct tgagggaatc aatagcagca tccgtgcctg cacctgcttt
     1261 caccattaac aggatgcgac gaggcgtttc cagagattca acaaactctt tcaccgtata
     1321 gtaaggaacc agtttcttgc ctggattttc ggcaatcacc tcttctgtct tctcacggga
     1381 acggttgaaa atagagacgg tataaccacg gctttcgatg ttgagcgcaa ggttgcgccc
     1441 catcactgcc ataccgacta cgccgatctg ttgctttgac attgtttact cctatcagtg
     1501 gcgcaacgca caacaaagtg cagtttgaat gtattcatta tatttatcaa cattttgttg
     1561 attcatagtt aataggattt tatagactta agaacactat tggcccatat aatatcggat
     1621 tgtgctgaat atgtgttctt tccacagcga atccaagtca gtcaatgatt cggatcttaa
     1681 gtacatttta ttatcagaga tttgaactct attactgata atggcctttt caactt
//
LOCUS       DAZFJL010000276         1723 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZFJL010000276 DAZFJL010000000
VERSION     DAZFJL010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.279"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            93..1235
                     /locus_tag="ACHJ8C_004695"
     CDS             93..1235
                     /locus_tag="ACHJ8C_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFO5554387.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGVELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESVTGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISAGLPLVAIGKGLVINPEWV
                     TLAESGRSHEIQTALNPQRVPELTIPDKLWDQIQASKGTGWFPLMD"
BASE COUNT          532 a          367 c          367 g          457 t
ORIGIN      
        1 aattccatta aaggaaaaaa gcatcaaaaa gtagcaactc aacttattaa gattatacgc
       61 attaaaatca gataaacatt tgaggtattg ttatgacaaa taaacaccca tcattgtttt
      121 ctccttttat gctgacagaa aaaataaagc tacgtaatcg tatcgttatg gctcctatga
      181 caacctggtc agctaatcct gatggaacca tctctgagca agaactcgaa ttttataaac
      241 gtcgctcaca aaatgttggt ttagtaataa caggatgtac ctatgtcacg ccaagcggca
      301 ttgggtttac tcatgaattt gccgcgtatg acgaccgttt cattaacagc cttgaaaagt
      361 tagcagctgc agccaaaagt ggcggtgctc cggctatttt gcagattttc cacgccggta
      421 ataaagccat cccggagctt gtgccaaata acgatgttat tagtgccagc gcatcaagcg
      481 taaaatctgg tgactttatg aagagagtgg tacaaagcag ggaaatgact gaaaacgaaa
      541 ttcaggaaac cattagagca ttcggagatg tcacaaaaag ggcgattaaa gcaggttttg
      601 atggcgttga acttcatggt gcgcacggat tccttctgca gaatttcttc tcccctctat
      661 ttaaccagcg taatgaccga tggggaggag atcttgaagg acgtatgcgc ttcccgctgg
      721 ccgtattaca ggaagtaaaa aatgtagtct atgaatatgc gacaaagccc tttgctatcg
      781 gttacagaat atctcctgaa gaatctgtaa caggggggct gcggatagag gatacttata
      841 aactccttga tcgactcatt tcatccggta tttcgtatat acatacctcg ctggtcagta
      901 ttaacgatag ctaccctgtt gagtcgccaa atggcccccg aactattgaa ttgattttaa
      961 atcatattgc aggcagagtg ccggtaatcg ccgcaggtaa aatacgcaca ccgtctcagg
     1021 ctcaagaagc aatttccgct ggcctgccac ttgttgcgat cggtaaaggg ctggtgataa
     1081 atccagaatg ggtgacttta gctgaatcag gtagaagtca tgaaattcaa acagccctta
     1141 atccacaacg cgttcctgaa ttaaccattc cggataaact atgggatcaa attcaggcat
     1201 cgaaaggaac tggctggttt ccgttaatgg attaaatatc gattactctc ccgagagtaa
     1261 aactatcaaa tcaatgagta accgatacag cctcgcaaat tctttcagat aattaacccg
     1321 tcactgcggc acgttctctc gcaaggcgtg cctgcagtgt aatccagtat gtatccggca
     1381 tagttgacgg tttaatagac tcaattcctc tccctggtta cctacaacga tatcttgtat
     1441 aacaccccag gagatacgac gttacgatga gatgcgatat gctcctcagc gccaatcaac
     1501 gccaggactg ggccagtctt gagatcagac tcattcccgt aactgatttc aggcgacata
     1561 tatcaatgac gttggggcaa taaaagatag ccgatgtaat acatctattc aaatttgtga
     1621 tacatgtcaa aaaattggtt gaccaaactc gttatttata taagggcact tacgaagtgc
     1681 actctttttt aaagcgagga agtaccaatg aaagagaata aag
//
LOCUS       DAZFJL010000277         1641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZFJL010000277 DAZFJL010000000
VERSION     DAZFJL010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.280"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..250)
                     /gene="gadE"
                     /locus_tag="ACHJ8C_004696"
     CDS             complement(<1..250)
                     /gene="gadE"
                     /locus_tag="ACHJ8C_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO5554388.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAK"
     gene            complement(1049..1621)
                     /gene="hdeD"
                     /locus_tag="ACHJ8C_004697"
     CDS             complement(1049..1621)
                     /gene="hdeD"
                     /locus_tag="ACHJ8C_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO5554389.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKKQ"
BASE COUNT          536 a          347 c          294 g          464 t
ORIGIN      
        1 ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc tcggcatcta
       61 atttctccag gaatttaatc gcttcttcat caaggatatg gttgtgatag gtgtcaatga
      121 tcaccttgaa gggtttattg cctacttttt taatctctga cagggaattg atttttatca
      181 tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct ttcgtcatga
      241 gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc ttttcattat
      301 ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac tttgcccttc
      361 gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc acgcactcca
      421 tatattggtc ctaagtaaga ttcctggttg ttatcagttt gtaactagta aacaaaaggt
      481 taacttttgc ggattactgc ccaagaataa gttatcaata accagtttgt gatctctgaa
      541 gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt gtttcgactt
      601 aggctggcaa ttggattgcc agctatcttt taacgcattg aaattacctg attaaataac
      661 tcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta tgcaatgtaa
      721 tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca tagcatcaac
      781 gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa cctgaccacg
      841 gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggaacg aaaaaaatca
      901 gattaaattc agtaggttga aataatcaac agcaggcaat tgtttcaatg atagtgcgta
      961 attgaactga aacactgtat agctaagaga gtattaactg gatcatagtc acttaatgtg
     1021 actatgatcc aggtggaaat agggataatt attgcttttt aacgaacaaa ctggcgaagc
     1081 tgaacagact ggcggcgcta aatatcagtt caattcccac cagtgtggaa accagcgtta
     1141 ccgacaccat cggcgtagca ccaaggaata tccaggcaat gacgatatcc agcacaccaa
     1201 taacgagctg tagccaactg cctttcattg aacgctgacg ataccaactc atcaggcgaa
     1261 taacccctgc cacacagaac aaaccggcaa taaatgccgc aatggcaaaa atgcccagct
     1321 ctggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg aggaaaccgg
     1381 ataataccgg ccagaaatta tgactgcggt tgctgaatag cccgacaata agcgcaatgc
     1441 ccgagcagat taataatgca cccactactg tgcttaaaat gtcgccagag acgaacggaa
     1501 aactgataca cagcaacccg acgataaaca gcagcacggc aataaactgg attgctctgc
     1561 gatgtttttt aagcatctcc agatcaaact tcaaaattgt tgccttatct atatataaca
     1621 tagaaccacc ctataaaatt a
//
LOCUS       DAZFJL010000278         1634 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFJL010000278 DAZFJL010000000
VERSION     DAZFJL010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.281"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            95..391
                     /locus_tag="ACHJ8C_004698"
     CDS             95..391
                     /locus_tag="ACHJ8C_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepZ/EspZ family type III secretion system
                     protein"
                     /protein_id="HFO5554390.1"
                     /translation="MEAANLSPSGAVLPLATTINGNNPVDENTGVMQPEGGTSRSVKV
                     LGGVLMSVGVLAAIGTGIAAMCVDDPSQKLGLGIAAGVLGGVTTVAGGLAMRYA"
     gene            473..850
                     /gene="sctI"
                     /locus_tag="ACHJ8C_004699"
     CDS             473..850
                     /gene="sctI"
                     /locus_tag="ACHJ8C_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFO5554391.1"
                     /translation="MNNINQSENINIQLNKASQTNVVDEHIPLASTPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNTDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            853..1425
                     /gene="escJ"
                     /locus_tag="ACHJ8C_004700"
     CDS             853..1425
                     /gene="escJ"
                     /locus_tag="ACHJ8C_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HFO5554392.1"
                     /translation="MKKHIKNLFLLAAICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNMTLSVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            1431..>1634
                     /gene="sepD"
                     /locus_tag="ACHJ8C_004701"
     CDS             1431..>1634
                     /gene="sepD"
                     /locus_tag="ACHJ8C_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HFO5554393.1"
                     /translation="MNNNNGIAKNDCDWLTALDFVKDVNGSPTHLTFYIYQKNAFLHD
                     FGNYWVLYIELSGDFRQIPTDTFI"
BASE COUNT          554 a          306 c          330 g          444 t
ORIGIN      
        1 tgcaactatt taaataatat aatagttgcc tatgggataa tttggttatt tataaccagg
       61 taaatgtcta aattagacaa aaggagaaag atcaatggaa gcagcaaatt taagtccttc
      121 tggtgcagta ttaccactgg caaccacaat caatggaaat aatccagtgg atgagaatac
      181 aggagtgatg caaccagaag gtggaacaag tcgcagcgtt aaagtcctgg gtggagtttt
      241 aatgagtgta ggtgttctag ccgctatcgg tactggtatc gcagcaatgt gtgttgacga
      301 cccttcacaa aaattaggct taggaatcgc agccggtgta ttaggtggcg taactactgt
      361 tgccggcgga ttagcgatga gatatgccta attatcgcat cgcataaaaa atagagaggt
      421 aatggatgca ttatgccatt gcctctctca tgaaaagaga ctaacagtaa atatgaataa
      481 tattaatcaa tcggaaaaca ttaatattca gttaaataaa gcatcccaaa caaatgtcgt
      541 tgatgagcat atcccgttag catcaacccc ctctgcagcc ggagcggctc agtttcttga
      601 tcaattactc cctaaaacag cgggagtgtc ttccccagaa caagtgttga ttgaagaaat
      661 taaaaagcga catcttgcaa caatgaacac cgatctcagt ttcgatgcac tatctgcagg
      721 tgggctctcg cccgaagatg tactcacttt acaaaagaat gtgctcaacg caaacgtcaa
      781 tgttgatgtc gtatctaagt tagcaagcct cctttcaaca tcggttacaa aattagtttc
      841 gatgcaataa aaatgaaaaa acacattaaa aacctttttt tattggctgc tatttgtttg
      901 acagtggcat gtaaagagca attgtataca ggattaacag aaaaagaagc taatcagatg
      961 caagcactgt tgctatccaa tgatgtcaat gtttccaaag aaatggacaa aagtggaaat
     1021 atgacactca gtgtggaaaa agaggatttt gttcgcgcaa taacgatttt aaataataac
     1081 ggcttcccga aaaagaaatt tgcagatatc gaggttattt tcccacccag ccagttagtg
     1141 gcatcgccat cacaagaaaa tgcaaaaatc aactatctca aagaacaaga catcgaaagg
     1201 ttgctaagta aaatacccgg tgttatcgat tgcagcgttt cattaaatgt taacaataac
     1261 gaaagtcaac catcctcagc agccgtttta gtcatcagct cgccagaggt taacttagca
     1321 ccatcggtca ttcagataaa aaacctggtt aagaatagtg tcgacgatct caaattagaa
     1381 aatattagtg ttgttatcaa gtcatcctca ggacaggacg ggtaatatgt atgaacaata
     1441 ataatggcat agcaaagaat gattgtgact ggctaacggc ccttgatttt gtaaaggatg
     1501 tcaacggttc accaacgcat ctcacttttt atatctacca aaaaaatgct ttcttgcacg
     1561 attttggaaa ctactgggtt ctatatatcg agcttagtgg tgattttcgt cagattccaa
     1621 cagatacatt tatc
//
LOCUS       DAZFJL010000279         1587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZFJL010000279 DAZFJL010000000
VERSION     DAZFJL010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.282"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(229..420)
                     /locus_tag="ACHJ8C_004702"
     CDS             complement(229..420)
                     /locus_tag="ACHJ8C_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554394.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVV
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(417..839)
                     /locus_tag="ACHJ8C_004703"
     CDS             complement(417..839)
                     /locus_tag="ACHJ8C_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO5554395.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFVVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRASRHVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEKMS"
     gene            complement(886..1311)
                     /locus_tag="ACHJ8C_004704"
     CDS             complement(886..1311)
                     /locus_tag="ACHJ8C_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5554396.1"
                     /translation="MQYAKSVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKQMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            1391..>1587
                     /locus_tag="ACHJ8C_004705"
     CDS             1391..>1587
                     /locus_tag="ACHJ8C_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554397.1"
                     /translation="MHRAQGKPQPDAHGWRELEGETAKSERVNDRKITTDGKEGPGDR
                     SGEAYPCIPASPAKMEARHSP"
BASE COUNT          375 a          487 c          414 g          311 t
ORIGIN      
        1 gcagacagaa caacactgca gtagtcagaa tcagagcagc agataaagga acaacaacat
       61 caggaacagt tccataacat aagaaagaaa cactcctgag tcagacacat cacagacggg
      121 aagggtaacg ggcgacgcgc cagcgtggcc cgcttccctt ccggcaggcg ccagcctgcc
      181 aggggttttc gcgcgggctg acaggcggcc tccaccgcct gccctgtatt acctgtttct
      241 gctgccctga aggatatagt gaccgcccag gaaaaccgcc tgctgattca ccacaaaatc
      301 gcagatttca tcaacaatct gcgcggtagt gagcggtttc tgccccatct cacgacgccg
      361 cgcattaatc acggccctgt aattacgcag aatttcgcgg gtttctgtgc tgatattcat
      421 gacatcttct ccccgtccag cgcctgtaac acaacgtcca ttgcttcgcg ttcctccctg
      481 acgaatgcgt caccgtcacc cccgttctca ctcccgacgg catacaggcg cttcatttca
      541 ctgcctgcca gcgccatcac tttttccagc atcaccgccg ggactgtcac atgacggctg
      601 gcgcgctttt cctccatcag ttcacgaaca acgtcgtgaa caacgctgac atccgcacca
      661 tgctcaaagg catcgtccag ccgctgcatg acggtttcca gctcatcgtc tgtcagactc
      721 cagtcctggg ctatgtggcg cacatcccca cgggtcagca ccacaacaaa aacgcattca
      781 ctgtccggca cgtctgtatc cagtacatcg cggataattt ctttaacagt acagtacatg
      841 ctctcgtctc ctttacaggg gcggcattgc cgccccgtcc gttaatcagt ccagtgccgc
      901 gtaaatcgcg ttgcattcag ggtgaaattc gatgtgacgc cacaactgcg catcgcgcat
      961 ccggtaaagc tgggtcagtc ctgcatcccc gctgtcgtgg tacagccaca actggcggtt
     1021 aatcacaagg gaagtcagaa tgatgcctgc ggcatcagca ctcacggtac ggtcaaacca
     1081 gtttgcaccg ttgaccatgt ggaaacggtc gccatccggc atcatgtaac cgccaccgtc
     1141 cggcaggcgg atgaagtgcc agaagccgcc ttcatattcc ggcatcattt gtttcgccag
     1201 ggcgtacaca gacgcttcgg catacagaaa atccaggcca aacaggtaag gcagaaagga
     1261 gagacggtcg cattcttcaa cgttcagagt gacagatttt gcatactgca tgggatttcc
     1321 tccgttgttc acatattccg cgacaccggg cggtgccgtg cttccataaa agccgggcga
     1381 taaggcgggg atgcacaggg cgcaagggaa gccgcagcct gacgcacacg gctggcggga
     1441 gttggagggt gagaccgcga aatctgagcg ggtgaatgac cggaaaatca cgacagacgg
     1501 gaaggaggga ccgggtgacc gcagcggcga agcgtaccct tgcatccccg ccagcccggc
     1561 taagatggag gcacggcaca gcccaca
//
LOCUS       DAZFJL010000280         1558 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFJL010000280 DAZFJL010000000
VERSION     DAZFJL010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.283"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(40..654)
                     /gene="yagU"
                     /locus_tag="ACHJ8C_004706"
     CDS             complement(40..654)
                     /gene="yagU"
                     /locus_tag="ACHJ8C_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO5554398.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNSAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(917..1054)
                     /locus_tag="ACHJ8C_004707"
     CDS             complement(917..1054)
                     /locus_tag="ACHJ8C_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554399.1"
                     /translation="MVSGLICHGGFEPHAWGVASLMDVAVLVVAYLLVWLLVNRENHY
                     A"
     gene            1103..1261
                     /locus_tag="ACHJ8C_004708"
                     /pseudo
     CDS             1103..1261
                     /locus_tag="ACHJ8C_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          490 a          355 c          302 g          411 t
ORIGIN      
        1 aatggcgcta tatttgcgcc atttttatca ttcaaagcat tatctatttg agcctaaagg
       61 gatctcagga tctggctcat gggtaattct gtttcgtaaa tctctgcgaa taatttcaat
      121 agaccagaac cagactaaat gtccaaaaat ttcagagaca ttctcatacc acggaagatc
      181 aaagagaggt ggtgtaagtc ccatgagagg gaatgaaatc atatgaacaa aaagttgcgc
      241 taaagcacct gccagtaagc cctgccaaag tttaattttc ggaaatactt cggcgaccac
      301 acagtaacca acggcaaaaa ctatcgaaaa gattatgtgc gttacaccaa cccagttaaa
      361 gacatgcccg gcgaaggtat aaacagccga atttggatct gtcagtccca accaatcccg
      421 aagaaaaatg tacggaggat tgagaaagtt acgggagcaa tcaatttggc ctgcagccct
      481 gattaatgat tccggaccac acgctgcatt aaacatatca accggactac gtggtggcaa
      541 tggaacttct gccccccact taacaaatgc ggaaacaacg ccagcaatca gcccaatgaa
      601 tgcagcaaga ccataacgtc tgcggtttgg tggagtttgt tcaaatatat tcatatctac
      661 cctgcttgta ccattatgtt atacacctct ttaggagtat tcataaaaca aggcaaatgt
      721 aaagaactgt attgttttgt ataaccacat ggttacctaa tcgccaatta atataagctc
      781 cattatttct ccatattttt atgttagaag tgacaaggag aaatgtaatg tttctctcag
      841 agaatataaa tcattccttt tgcccacctt aatggtgggc ttttttatgc aaaacctgaa
      901 cgtcctgtgg tattcctcag gcataatgat tttcgcgatt aaccaacaac cagaccagca
      961 aatacgccac cacaagcaca gcaacatcca tcaggcttgc cacaccccat gcgtgcggct
     1021 caaagccgcc atggcatatt aatccgctta ccattttcga acagctgatt ggtttggtcc
     1081 ggtgtttccc tgcttgcact taaaaatgag cggataggaa actctcgaaa tggttcgtcg
     1141 atatgctcac ctcgcaccta atcaccttac cgaacacgca cggtaaatag attcgatcct
     1201 gaacccatcg gtcccaaatt tgtcccagtc aaaaaataag gaaggtacta atgatgtgta
     1261 acttattgat ttaaatggtg ccgataatag gagtcgaacc tacgaccttc gcattacgaa
     1321 tctgtagcac caatcataac tatctgtttt agcaagcatt aaccgcattc actaagctat
     1381 agttgatggc acaaacagaa agttgatgca tgatgttgtc atgtgtatgt cacaaatacg
     1441 gcacaacaat cttcaaacag tagtggtcaa gtaatactgg ccacggtttt acagtaaaaa
     1501 tggtacctgt tctcggactc ttccggagtc aacccaccgt tataatggtg aggtctga
//
LOCUS       DAZFJL010000281         1515 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFJL010000281 DAZFJL010000000
VERSION     DAZFJL010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.284"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..327
                     /locus_tag="ACHJ8C_004709"
     CDS             <1..327
                     /locus_tag="ACHJ8C_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HFO5554400.1"
                     /translation="VVAATFSLRLKGKPVLTTEPLKVKVDLNSTLQVSAGAKLEMVVE
                     AAGGVPPYSYVWKKGGSPVSGQTAATFSKASAVSGDAGAYTCEISDSASPVSKVTSTS
                     CAVTVS"
     gene            342..713
                     /locus_tag="ACHJ8C_004710"
     CDS             342..713
                     /locus_tag="ACHJ8C_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO5554401.1"
                     /translation="MTKNIRNLALATMSGFRHKTVDVPEWEGATVVLREPSAEAWLRW
                     QEIVKAKDDETPLSVAERARRNLEADVELFIDVLCDTGLQPVFSEDDREQVIAVYGPV
                     HARLLRQSLELISDAGEVKKK"
     gene            737..1015
                     /locus_tag="ACHJ8C_004711"
     CDS             737..1015
                     /locus_tag="ACHJ8C_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004967194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO5554402.1"
                     /translation="MMLALRMGRTLSELRREMSASEIMMWAEFDRFSPLGDERADIRA
                     AQIVSAVYGAQGVKVPLNDALLQWEQEQTEGASDPFAGLENALLIVSQ"
     gene            1061..>1515
                     /locus_tag="ACHJ8C_004712"
     CDS             1061..>1515
                     /locus_tag="ACHJ8C_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO5554403.1"
                     /translation="MATLRELIIKISANSRSFQSEISRASRMGQDYYRTMQNGGRQSA
                     AASREMRRALAEVTDQINTAKSSALNMAGAFAGAFATGHLISLADEWNSVNARLKQAS
                     QSSDDFQASQRELMAISQRTGTAFSDNASLFARSAASMREYGYSSEEVL"
BASE COUNT          346 a          343 c          451 g          375 t
ORIGIN      
        1 gttgtggctg caacgttttc cctgcgcctg aaaggtaaac ctgtgctgac gacagagccg
       61 ctgaaagtga aggtcgattt aaacagcacg ctgcaggttt ctgccggtgc gaaactcgaa
      121 atggtggttg aggctgccgg tggtgtgccg ccttattctt atgtctggaa gaaaggtggt
      181 tctcctgttt ccggacagac ggcggcaacg ttcagtaagg catccgcagt atccggtgat
      241 gccggtgcgt atacctgcga gatttctgat tcagcaagcc cggttagcaa ggtgacctcc
      301 acttcctgcg ctgttaccgt cagttaatga ggatagatgt gatgactaaa aatatccgta
      361 atctggcact ggcaacgatg tcggggtttc gccataaaac cgttgatgtg cctgaatggg
      421 aaggggcaac ggttgtatta cgggaacctt ctgcagaagc ctggttgcgc tggcaggaga
      481 tcgttaaagc aaaagatgat gagacaccgt tatccgttgc ggagcgcgcc cgccgaaatc
      541 tggaggcaga tgttgaactg ttcattgatg ttctgtgtga taccggactg caacctgtat
      601 tttcagagga tgatcgtgaa caggtgattg ccgtgtatgg cccggtgcat gcgcggcttc
      661 ttcggcagtc tctggaactg atcagtgatg ccggcgaggt taaaaaaaag tagcgcttcc
      721 ggggatgcgt tttctgatga tgctggcgct caggatgggg cgcacattgt cagagttacg
      781 ccgggaaatg tccgcatcag aaatcatgat gtgggcagaa tttgacaggt tcagcccgct
      841 gggtgatgag cgggctgata tccgggctgc ccagatagtt tctgcggttt acggtgcgca
      901 gggtgtcaaa gtaccactga atgatgcgct tcttcagtgg gaacaagagc agacagaagg
      961 cgcctcagat ccatttgccg gactggaaaa cgcgctttta atagtgtctc agtgagtcaa
     1021 cataaccgct tcggcggttt ttttcgtccg gagaatgagt gtggcgacat tacgtgaact
     1081 gattattaaa atctcggcaa attcccggtc attccagtca gagatctccc gggcttcgcg
     1141 tatggggcag gattactacc gcaccatgca gaacggaggc cgacagtccg ctgctgcatc
     1201 ccgtgaaatg cggcgtgcac tggcagaagt gacggatcag ataaatacag ctaaatcttc
     1261 ggcactgaat atggcggggg catttgccgg ggcttttgct accggtcatc ttatttctct
     1321 cgccgatgag tggaattcag taaatgcacg tctgaagcag gcctcacagt ccagtgatga
     1381 ttttcaggca tcacagcgtg aattaatggc gatcagccag agaacgggga cggcgttttc
     1441 tgataacgcc agcctttttg cccgttctgc agcttccatg cgggagtatg gttacagttc
     1501 tgaggaggta ctgaa
//
LOCUS       DAZFJL010000282         1495 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFJL010000282 DAZFJL010000000
VERSION     DAZFJL010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.285"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(312..>1495)
                     /locus_tag="ACHJ8C_004713"
     CDS             complement(312..>1495)
                     /locus_tag="ACHJ8C_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EspG domain-containing protein"
                     /protein_id="HFO5554404.1"
                     /translation="NGISQLRSIQSNSAQSNSAQSNSALFALESVLDVSSYLENNWSD
                     KPWAERILSYLGLSHCRSEYKDDDRLLLDVLSVLKPILPSSNEAGKVQASFSDGSRLT
                     ISVTKSELIEIAVTTPEGDEVSIFPERNKQDCLLKSLPVALHMPYIKKHHSLPEIEVN
                     GADGMRFLCNFAAKLSTAIVPYSEKTNPLSGVSPLNSIYADTFRGLGNSDVSINGVSL
                     SREAQQLLCHFIGLKDTGSSPPSNIINNGIDYDQAVILVSKSHVGEEQKALLTAVLCQ
                     PEFVAAICSSFYQSFTVPAFLLMHERIFLARQHFSEKSLVLPNAHFSINISNSSNGGL
                     YVFNNVGTFIMAPGERGDVVGVFTMRTSYEIPLGTRCEIPELVRVIKPIYSASEAYSR
                     N"
BASE COUNT          506 a          298 c          255 g          436 t
ORIGIN      
        1 caaaaaaaac accccctcga ttgtgatttt tattttaatg gtaacaaaca acaaaaaaat
       61 agataacaat aaaatacttc taataattaa caaaaaaaac cactctcaat gcaataaaac
      121 atgggagtac attataatat ttaattcctg agcacagttt cgttttgcat cccaaacatt
      181 gaacgtcagt atcggagagt ccaaaagagc cggtatcagt catattttat atctgtacac
      241 ctgagatgta ctcgttgcga cgaatacatc tcatttcaaa tttactgcat agtatctata
      301 gttgtgcagt atcaattcct cgaatatgct tcagatgcac tgtaaattgg tttaatcact
      361 cgaacgagct caggtatttc acacctggta cccaaaggta tttcataaga tgttctcata
      421 gtaaatacac cgacaacatc acctctctct cccggagcca tgatgaaggt acccacattg
      481 ttaaaaacat agagtccacc atttgaagaa ttcgatatat ttatactaaa atgtgcattt
      541 ggtaaaacta atgatttttc tgaaaaatgc tgacgcgcga gaaatattcg ctcatgcatt
      601 aacaaaaaag caggaacagt aaacgattga tagaaagagg aacatatagc agcaacaaat
      661 tcaggctgac acagtaccgc agtcaaaagg gccttttgtt cttctccaac atgactttta
      721 gagaccaaga taacagcctg atcataatca ataccgttat ttatgatgtt tgagggagga
      781 gaagagcctg tatctttaag accaataaaa tgacaaagaa gttgctgagc ttcacgagat
      841 aaacttacac cattaataga gacgtctgag tttccaagcc ctctgaacgt atctgcatat
      901 atagaattca gggggcttac gccagaaagg ggattcgttt tttcactata aggaactatt
      961 gctgttgaaa gtttggcagc aaaattacat aaaaaacgca tgccatcagc cccattcacc
     1021 tcaatttccg gtaaagaatg atgtttttta atatatggca tatgcaaagc aacaggtaat
     1081 gatttcagta aacaatcttg tttatttctt tcaggaaata tggaaacctc atccccttct
     1141 ggggtagtaa cagctatttc aatcagctca ctttttgtga ctgaaatggt tagcctggag
     1201 ccatccgaaa aacttgcctg cacttttcct gcttcattac tacttggtaa tatcggtttt
     1261 aatacactta atacatccaa taataatcgg tcgtcatctt tatactccga cctacaatgg
     1321 cttaaaccta agtagcttag aattcgctca gcccatggct tgtcagacca gttattttcc
     1381 aagtaagaac ttacgtctaa taccgattcc agagcgaata aagctgaatt tgactgagct
     1441 gaatttgact gagctgaatt tgactgaata gatctcagtt gagaaatgcc attta
//
LOCUS       DAZFJL010000283         1481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFJL010000283 DAZFJL010000000
VERSION     DAZFJL010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.286"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..123
                     /locus_tag="ACHJ8C_004714"
     CDS             <1..123
                     /locus_tag="ACHJ8C_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO5554405.1"
                     /translation="TRILRDPYTNKPFVGFYTTKRTGGMLVDSQAIKLLKIAAA"
     gene            175..363
                     /locus_tag="ACHJ8C_004715"
     CDS             175..363
                     /locus_tag="ACHJ8C_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554406.1"
                     /translation="MILKQDLKWSPDGMRVEIIRAGEYEDKELPERVREIATAAGIVS
                     EKRTPVARGADKPKKQHS"
     gene            375..680
                     /locus_tag="ACHJ8C_004716"
     CDS             375..680
                     /locus_tag="ACHJ8C_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO5554407.1"
                     /translation="MMPTLEELRVQCRIDDDNEQENSLLMMYLAAAREEAEKFLNRTL
                     YDETVSEQDTTGLVITPLIKLRLMQLVGYWYENREMQDAVPDFFYTGLRMYRFHPGT"
     gene            689..1027
                     /locus_tag="ACHJ8C_004717"
     CDS             689..1027
                     /locus_tag="ACHJ8C_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO5554408.1"
                     /translation="MQAGRLRDRVVILNATTVRSPSGHPVETMTEGATIWAEVKGISG
                     RERISGGAETAQATVRVWMRFRRDVTATSCLKVLTGAFKGAILSIDGPPIPDARATRL
                     EILCSQKGNV"
     gene            1024..1473
                     /locus_tag="ACHJ8C_004718"
     CDS             1024..1473
                     /locus_tag="ACHJ8C_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO5554409.1"
                     /translation="MMDFSLDFSGLADIARDLETLSRAENNKVLRDATRAGAEVMRDA
                     VVERAPERTGKLKKNVVVLTQRSKRRGEIISGVHIRGRNLRTGNSDNSMKASDPRNAF
                     YWRFVELGTINMPAHPFIRPAFDTTEELAAQIAIQRMNQAIDEVLSK"
BASE COUNT          387 a          329 c          432 g          333 t
ORIGIN      
        1 acccgcattc tgcgtgaccc gtacaccaat aaaccgtttg tcggttttta taccaccaaa
       61 cgcaccggcg gcatgctggt cgattcgcag gccatcaaac tgctgaagat tgcagcggcg
      121 taatcattca gggggcgcgg aaccgcgccc cctgttctga caggtgaaag aatcatgatc
      181 ctgaaacaag atctgaaatg gtcaccggac ggtatgcgtg ttgagattat tcgggccggt
      241 gagtatgaag ataaagaatt acccgaacgg gtacgcgaaa ttgccactgc agctgggatt
      301 gtctctgaaa agagaacacc tgttgcgcgg ggggctgata agcctaaaaa acagcattca
      361 tagaggttgc ccaaatgatg cccactctgg aagagcttcg tgttcagtgc cggattgatg
      421 atgacaatga acaggagaat tctcttctta tgatgtatct ggctgccgcc agggaagagg
      481 ctgaaaagtt tttaaaccgg acgctttacg atgaaactgt ttctgagcag gatacgaccg
      541 ggcttgtaat aacacctctg ataaaactgc gtcttatgca actggttggc tactggtacg
      601 agaacaggga aatgcaggat gcagtgcctg attttttcta taccggactg cggatgtatc
      661 ggtttcatcc cgggacatag gaggattcat gcaggcagga agattacgtg atcgtgtggt
      721 tattctgaat gccaccaccg ttcggtctcc atcagggcac cctgtggaaa caatgacgga
      781 aggggcaacc atatgggcag aagttaaggg gatcagtggc agggagagaa tatccggagg
      841 cgcagaaact gctcaggcta cagtgagggt ctggatgaga ttccggcgag atgtgacagc
      901 aacttcatgt ctgaaagtgc tgactggcgc attcaaaggc gcgattctga gtatagacgg
      961 tccgccgata ccggatgccc gtgccacccg gcttgagata ctctgttctc agaaggggaa
     1021 tgtgtgatgg atttcagtct tgatttttca ggtctggcgg atattgcacg ggatctggag
     1081 acgctcagca gggcagaaaa caataaggtg ctgcgcgatg ccacccgtgc cggtgctgaa
     1141 gttatgcggg atgcagttgt tgaacgtgcg ccggagcgaa ccgggaaact gaagaaaaat
     1201 gtggttgttc tcactcagcg ttcaaagcgt cggggggaaa ttatctcggg tgtccacatt
     1261 cgtggacgga acctgcgaac cggaaacagt gataacagca tgaaagccag cgatccccga
     1321 aatgcgtttt actggcgctt tgtggagctg ggaacgataa acatgcccgc gcatccgttc
     1381 attcgcccgg ctttcgatac gacagaggaa ctggcagcac agattgccat acagcgaatg
     1441 aatcaggcta ttgatgaggt cttaagtaaa tgagagagac c
//
LOCUS       DAZFJL010000284         1479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFJL010000284 DAZFJL010000000
VERSION     DAZFJL010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.287"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..339)
                     /locus_tag="ACHJ8C_004719"
     CDS             complement(<1..339)
                     /locus_tag="ACHJ8C_004719"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554410.1"
                     /translation="MNDLNSVFSFSQTDVNNALNTFDDQLKDIVNSDDEDSTDDYDSL
                     ISGFIDKLPKEWLFIDFDKLFFPSQQQRQIGAQPIEVNFSFSLPVIGVVHFSIDTTDF
                     SSAYDLLLRPV"
     gene            404..967
                     /locus_tag="ACHJ8C_004720"
     CDS             404..967
                     /locus_tag="ACHJ8C_004720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554411.1"
                     /translation="MLPLDEPPEVLPLDEPPEVLPPDEPPEVLPPDEPPEVLPPEVLP
                     PDEPPEVLPLDDPPEVLPPEEPPEVLPPEEPPEVLPPDEPPEVLPPDEPPEVLPPDEP
                     PEVLPPDEPPEVLPPDEPPEVLPPDEPPEVLPPEEPPEVLPPEEPPEVLPPDEPPELS
                     PPSVLPLFPLSSVSPLSPLSSVSSLSE"
     gene            complement(1042..1293)
                     /locus_tag="ACHJ8C_004721"
                     /pseudo
     CDS             complement(1042..1293)
                     /locus_tag="ACHJ8C_004721"
                     /inference="COORDINATES: protein motif:HMM:NF017195.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          475 a          390 c          311 g          303 t
ORIGIN      
        1 aacaggcctc aacaacaaat catatgctga tgaaaaatcg gttgtgtcaa ttgaaaaatg
       61 cacaacccca atcacaggaa gagaaaatga aaaattaacc tcaataggtt gcgcacctat
      121 ttgccgttgt tgttgagaag ggaaaaataa cttatcaaaa tcaataaaca accactcctt
      181 cggtaactta tcaataaatc cagaaattaa agaatcataa tcatctgttg aatcttcatc
      241 atcactattg acaatatctt tcaactggtc atcaaaagta ttcagtgcat tatttacatc
      301 agtctgagaa aaagaaaata cactgttcag atcattcact gaactccaga ttctctgcaa
      361 ccagccaaga atatcaccaa gccagccatc atcatcaccg gaagtgctgc cactggatga
      421 accaccggaa gtactgccac tggatgaacc gccggaagta ctgccaccgg acgaaccgcc
      481 ggaagtgctg ccaccggatg aaccaccgga agtgctgccg ccggaagtgc tgccgccgga
      541 cgaaccgccg gaagtgctgc cactggatga tccaccggaa gtgctgccac cggaggaacc
      601 gccggaagtg ctgccaccgg aggaaccgcc ggaagtgctg ccaccggatg aaccgccgga
      661 agtgctgcca ccggatgaac cgccagaagt gctaccaccg gatgaaccgc cagaagtgct
      721 gccaccggac gaaccgccgg aagtgctgcc gccggacgaa ccgccggaag tactgccacc
      781 agatgaaccg ccggaagtac tgccaccgga agaaccgccg gaagtactgc cgccggagga
      841 accgccagaa gtgctgccac cggacgaacc gccggaatta tcaccaccgt cagtattacc
      901 gttattccct ttatcatcag tatctccgct atcaccttta tcttctgtat cttctttatc
      961 agaataagcc ggatcaacag ttacaggttt ccaggtagcg gtacaagaat ctgtaccatc
     1021 gggacaaata atagcaacac ccgttgcctc ataaatgcat ccatgaatgg tgacatattt
     1081 attcccttta tcatccttat aaacacctga caaagaaaca ttactaagct caggacggga
     1141 taaacaaacc tcttccgtgg gtttagctga atacgaagca ccaaaatatt gaccgtaaaa
     1201 gctatcctta cactgagaat aaaccttaac accatcctca aaatggtaag acttataaga
     1261 gatccagtta cctgtaacca gtaaaaatgg catacccaaa agatgattag tatcgctgta
     1321 agacgtagta caataaaacg tatcacctga gcaggatgca tatccagtag tacagggtgg
     1381 agctacaccg gcccttgcaa cacgggtacc ggaacctgca tcaccaagaa attctgtcgt
     1441 tgaagctgca tatgcagaat taaatgaaaa aacagcaac
//
LOCUS       DAZFJL010000285         1457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFJL010000285 DAZFJL010000000
VERSION     DAZFJL010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.288"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            76..1353
                     /locus_tag="ACHJ8C_004722"
     CDS             76..1353
                     /locus_tag="ACHJ8C_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5554412.1"
                     /translation="MNKIKNYRWHMIALVCFITVINYLDRTALGIAAPTIMETTGITK
                     EQYSWIVSAFQLAYTLGQPIMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLA
                     FMRALMGFSEASAIPAGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMF
                     HSWQFAFIVSGSLALLAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKT
                     SIKHILSQRNFWGIGIARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLG
                     DLGCLASGFVAKFFHDRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIG
                     AFSHQCLSTVAATLGGDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPF
                     FIALAFFDIIGAIALWMLIKVKDEEPQVQLATS"
BASE COUNT          368 a          310 c          337 g          442 t
ORIGIN      
        1 gtacctgatt tttattgcct attacatgga aataccaaca accagggttt attaatataa
       61 ttaaaaggtt taaaaatgaa taaaataaag aattatcgat ggcatatgat tgccctcgta
      121 tgctttatca ctgtaatcaa ttatctggac agaacggcgt taggtattgc ggctccaacg
      181 attatggaga caaccggaat aaccaaagaa caatattcat ggattgtcag tgcattccag
      241 ttggcctata cattggggca accgataatg ggcttcttta ttgataccgt gggtctgaag
      301 ttaagttttg cgatatgtgc cgcaatctgg ggcctggcga caatggggca tgccttgaca
      361 ggaacatggt caggtctggc atttatgcgc gccctgatgg gtttcagcga agcatcggca
      421 attcctgcgg gtgtaaaaac cgcatcaaca tggttcccgg caaaagagcg tggcgtggcg
      481 acaggtgttt tcaatatggg cacctcactc ggcgcgatgc ttgctccacc gttgattgcc
      541 tggtgcatta tgtttcatag ctggcaattt gcgtttattg tctcaggtag ccttgcttta
      601 ctcgcggctt tattttggtt cttttgttat aaagatccaa aagatgctaa acgcctttct
      661 gatgaagagc gccactatat tgagtctggg caagaacagc atcttaaaac agataagaaa
      721 gaaaaaacgt caatcaagca tatcctcagc caacgtaatt tctgggggat tggcatcgcg
      781 cgttttctcg ctgacccggc atggggaacc attaacttct gggtaccgat tttcttcgtc
      841 gaaacgctgc attttagcct gaaagaaatt gccatgttcg tctggctacc tttcctgctg
      901 ggcgatctcg gctgtttagc cagtggtttt gtcgcgaagt tcttccacga tcgcggcgtg
      961 agtttaatta actcacgaag aattaccttc actattgcag ccgtcattat gatgacgatt
     1021 ggtctggtga gtattgtcga aaatccctac atcgccgtat tactgattag cattggcgca
     1081 ttctcgcatc aatgtctttc tactgttgca gcaactctgg gcggtgacct attcaaaaaa
     1141 gacgaagtag ctaccgctgt gggtatggca ggagcctgtg catggagcgg tcagttgatt
     1201 ttcaacctgt ttatcggggc attcgttcac attattggct tcgcgccgtt ctttattgcc
     1261 ctggctttct ttgacattat tggcgccatt gcgctgtgga tgcttatcaa agttaaagac
     1321 gaagaaccac aagtacagtt agcgacaagc taattatttt acatagaaac gctataattt
     1381 agctttgtag cgtttctatt tttctcataa attaaacgtc tcttccaaaa cgttcagtat
     1441 ctactggcat tgcttcg
//
LOCUS       DAZFJL010000286         1442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFJL010000286 DAZFJL010000000
VERSION     DAZFJL010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.289"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(66..1442)
                     /locus_tag="ACHJ8C_004723"
                     /pseudo
     CDS             complement(66..1442)
                     /locus_tag="ACHJ8C_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768545.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          374 a          355 c          268 g          445 t
ORIGIN      
        1 aaaataatat ttcatatgtt accgcgtcga tgaattcgac gcggtttttt agctaacggg
       61 cgggatcaga acttatattt caggcccacc atcattgcgg tgtcattgta gccattatca
      121 cccagctgca cgcccacatt accccagacg ctggccgccg gattcagatt gccatttacg
      181 ccatgtttca tttcccccag attacgcgcc ccgtcttgtt caactttcac accgtttatt
      241 gagacagcaa aatctttact gttatggatc ccgttcattt caacaaaagg ttcaaagtcg
      301 tcaccgtgcg gaccgctgtt ttctcctgag tacgaataaa ggtgtggaaa ccaagtcggg
      361 tctggatatt gttatttgcg tcattctcaa tacgtgagcc attggcttcc gtgtgcacat
      421 tatgatcgac gcccatccag atggcctgtg ccgaggtcgt tctcaactgt ccgttgctgt
      481 cacaccaggc attatgcccg ccaatttgac gtagccaaaa tcggcttgaa cgctcctgtt
      541 cagttacagg atcgatatag cgcgtttcac ctgcacgatc gttcagacgc ataacaaaaa
      601 gagtgttagc ggctgccagg ttggcgacgt aactgccagc ttcgggtcgc aggacggggg
      661 taggcgtttc tgttgattct gaagtgacat tggaaaaaaa taactctcta ccattgtata
      721 tttttgcaat gggggcttta gtgccatttg ttgtaataga acttttttat atcaatgtta
      781 ccttgtcgaa tagcaactgc ataacttcca tcagccacag tttcaagtac cacataatct
      841 aaattaatat atgctttttt cccattagca taagcaccat agctattaat accattagtt
      901 gttatggaac caccattcat ttttaaatca gcacggctaa taacttcgta actgttagaa
      961 tcacaaactt catcatctac agcatcgaca catgttcgag actctcctgg acttgaacat
     1021 gcaagaactc ctactgcatt tttccccata ggtaacaatg ttcgttttat ctgtggtttc
     1081 aagactggtg ttattttcca cagtcccaga atcattaatc tgacttaaaa gtcccgctga
     1141 atatgccccc tcgtctcgac tgaattatca ccattcataa cgatggtgcc accaaattca
     1201 ctgactactg cgctggctgt tatccctcca ttttctttgt cagatacgct ttcagagcgt
     1261 cttaatattg ctatcgctta tatataatac tttgttttgg ctatcaacag aaataccata
     1321 agatccatca ccactagtca tcacactggc attatgtata tatccagtta caccgtcatc
     1381 cactttcaca gcatggcttt cgtcttaagt gattagatac tccccattac ttgtagaagt
     1441 at
//
LOCUS       DAZFJL010000287         1429 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFJL010000287 DAZFJL010000000
VERSION     DAZFJL010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.290"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..54)
                     /locus_tag="ACHJ8C_004724"
     CDS             complement(<1..54)
                     /locus_tag="ACHJ8C_004724"
                     /inference="COORDINATES: protein motif:HMM:NF018510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554413.1"
                     /translation="MDLRVKTGAEAIRALSTQ"
     gene            complement(39..782)
                     /locus_tag="ACHJ8C_004725"
     CDS             complement(39..782)
                     /locus_tag="ACHJ8C_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5554414.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     VYFRMAPEDWLHAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FCCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            complement(788..1429)
                     /locus_tag="ACHJ8C_004726"
     CDS             complement(788..1429)
                     /locus_tag="ACHJ8C_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5554415.1"
                     /translation="MVVLWEVDLTEVGGERYFFCNEQNEKGESVTWQGRQYQAYPIQG
                     SGFEMNGKGASARPTLKVSNLHGMVTGMAEDLQSLVGGTVVRRKVYARFLDAVNFVNG
                     NSEADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGRIMLANTCTWTYRG
                     DECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGFLSINKLSQ"
BASE COUNT          312 a          466 c          356 g          295 t
ORIGIN      
        1 ctgtgtggac agtgcgcgga tggcttcagc ccccgttttc acacgaaggt cgatgcggcg
       61 gccaaatcgt tgcaaatccc cgtaaaggca gatgcgtgcc atgcccggtg acgccagagg
      121 gagtgtgtgc gtcgctgcca tttgtcggta tacctctctc gtttgctcag ttgttcagga
      181 atatggtgca gcagctcgcc atcaccacag taaatggcgg catgattcgg caccgatgaa
      241 ccaaaacagc acagcagcac atcgcccggc tgcgccgctg acaacggcac ctgatacagc
      301 cccgttgcct ccagattatc cagatagaga ttctgaccgt gacgccacca gtcatcctcg
      361 cgatgaaaat ccggcatctc aatccccgcc agatggtaag catcccggaa cagcgtgtaa
      421 cagtccgtca ccccgtgctc aaagcgccgc ccggtgagat gcggcacaca gcagaactta
      481 tgaatcgccc cccggcagac cagccaccac ggcaaatcac tctgcacctg cagccgccgg
      541 tcagcctcac tcagccaggg cagaccaccg gggtgactgt ggaccagcgc cacaatctca
      601 ccctgcatct ctgcatgcag ccagtcctcc ggagccatcc ggaaatacac ctccggctca
      661 ccggaaatat tcacgcaggg aaaatatctt tccccctccg gcgtgcttac cacgaagccg
      721 cacgactccg ctggcgcaca tcgccgggcg tgcgccagaa tcgctgattc tgtctgtgtc
      781 atggtattta ctgcgaaagt ttgttaatgg aaaggaagcc gccaaagttg ccgacgttat
      841 tgcggaactt acaaccgctc aggcatttgc tgcatttatc cttcgtgata tcggacgttg
      901 gctggtcata ttcatccgcg accgccggac cactataacc gcactcatcg ccgcgatagg
      961 tccaggtgca ggtgttggcc agcatgatac gtcccggaaa aacagcgccg tccgtttccg
     1021 tcggtgtgga caacacaaag gaggcgctga ccgcactcag ttcgctgcac tgctcgatgc
     1081 gccagcggct gatcacctcc tgctccggat cagcttcgct gtttccgttg acgaagttca
     1141 ccgcatccag aaaacgggcg taaaccttac gccggaccac cgttccgccg accagactct
     1201 gcagatcttc cgccatcccg gtgaccatac cgtgcagatt agagactttc agtgttggtc
     1261 tggcactggc tcctttgcca ttcatctcaa atccgctccc ctgtatggga tacgcctgat
     1321 actgccgccc ctgccaggtg actgactcac ctttttcgtt ctgctcatta cagaaaaaat
     1381 aacgctcgcc accgacttct gtcagatcga cttcccagag cacgaccag
//
LOCUS       DAZFJL010000288         1429 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFJL010000288 DAZFJL010000000
VERSION     DAZFJL010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.291"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..54)
                     /locus_tag="ACHJ8C_004727"
     CDS             complement(<1..54)
                     /locus_tag="ACHJ8C_004727"
                     /inference="COORDINATES: protein motif:HMM:NF018510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554416.1"
                     /translation="MDLRVKTGAEAIRALATQ"
     gene            complement(39..782)
                     /locus_tag="ACHJ8C_004728"
     CDS             complement(39..782)
                     /locus_tag="ACHJ8C_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5554417.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFYREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            complement(788..1429)
                     /locus_tag="ACHJ8C_004729"
     CDS             complement(788..1429)
                     /locus_tag="ACHJ8C_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5554418.1"
                     /translation="MVVLWEVDLTEVGGERYFFCNEQNEKGEPVIWQGRQYQAYPIQG
                     SGFEMNGKGASARPTLKVSNLHGMVTGMAEDLQSLVGGTVVRRKVYARFLDAVNFVNG
                     NSEADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGRIMLANTCTWTYRG
                     DECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGFLSINKLSQ"
BASE COUNT          306 a          460 c          364 g          299 t
ORIGIN      
        1 ctgtgtggcc agtgcgcgga tggcttcagc ccccgttttc acacgaaggt cgatgcggcg
       61 gccaaatcgt tgcaaatccc cgtaaaggca gatgcgtgcc atgcccggtg acgccagagg
      121 gagtgtgtgc gtcgctgcca tttgtcggta tacctctctc gtttgctcag ttgttcagga
      181 atatggtgca gcagctcgcc gtcaccacag taaatggcgg catgattcgg caccgatgaa
      241 ccaaaacagc acagcagcac atcgcccggt tgtgctgatg acaacggcac ctgatacagc
      301 cctgtggcct ccagattatc cagatagaga ttctggccgt tacgccacca gtcatcctca
      361 cgataaaagt ccggcatctc aatccccgcc agatgataag catcccggaa cagtgtgtaa
      421 cagtccgtca caccgtgctc aaagcgccgc ccggtgagat gcggcacaca gcggaactta
      481 tgaatcgtcc cccggcagac cagccaccac ggcaaatcac tctgcacctg cagccgccgg
      541 tcggcctcac tcagccaggg cagaccaccg gggtggctgt ggaccagcgc cacaatctca
      601 ccctgcattt ctgcctgcag ccagtcttcc ggcgacatac ggaaatacgc ctccggctca
      661 ccggagatat tcacgcaggg gaaatatctt tccccctccg gcgttctcac cacgaagccg
      721 cacgactccg ctggcgcaca tcgccgggcg tgcgccagaa tcgctgattc tgtctctgtc
      781 atgggattta ctgcgaaagt ttgttaatgg aaaggaagcc gccaaagttg ccgacgttat
      841 tgcggaactt acaaccgctc aggcatttgc tgcatttatc cttcgtgata tcggacgttg
      901 gctggtcata ttcatccgcg acagccggac cgtgataacc gcactcatcg ccgcgatagg
      961 tccaggtgca ggtgttggcc agcatgatac gtcccggaaa aacagcgcca tccgtttccg
     1021 tcggtgtgga caacacaaag gaggcgctga ccgcactcag ttcgctgcac tgctcgatgc
     1081 gccagcggct gatcacctcc tgctccggat cagcttcgct gtttccgttg acgaagttca
     1141 ccgcatccag aaaacgggcg taaaccttac gcctgaccac cgttccgccg accagactct
     1201 gcaggtcttc cgccatcccg gtgaccatgc cgtgcagatt agagactttc agcgttggtc
     1261 ttgcactggc tcctttgccg ttcatctcaa agccgcttcc ctgaatgggg tacgcctgat
     1321 actgccgccc ctgccagatg accggctcac ctttttcgtt ctgctcatta caaaaaaaat
     1381 aacgctcgcc accgacctcc gtcagatcga cttcccagag cacgaccag
//
LOCUS       DAZFJL010000289         1428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFJL010000289 DAZFJL010000000
VERSION     DAZFJL010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.292"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(420..680)
                     /locus_tag="ACHJ8C_004730"
                     /pseudo
     CDS             complement(420..680)
                     /locus_tag="ACHJ8C_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(757..>1428)
                     /locus_tag="ACHJ8C_004731"
     CDS             complement(757..>1428)
                     /locus_tag="ACHJ8C_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554419.1"
                     /translation="KFLEENLILNPQCYAHHDETSLITVNITREDGLLRLKEMEDDMA
                     GSDYILFTGMRDTDSPERFDAPAVISLGNQCISLRRNDISQTIQQSSPLWLTNQQSGC
                     SVLIVRHGLSESGEQQYSMIHMRPRDGNDFIDELTPELYANTQEVLLERDIQRTLANT
                     FPNEHPEAFILIPSAENFIVDGNCIQLIGIGNEQGEFDFYRQVYPVTGGEHQVEALMW
                     THLPA"
BASE COUNT          409 a          344 c          232 g          443 t
ORIGIN      
        1 atttatgtaa acaatattct gttaaaggtt gtattgtttg tatcatgacc gtcaccttta
       61 cttttgatac ttctattgac tctcacctct ggtagcatcc tgcaagaacc ttatttctgt
      121 aggagataca cggggtatgt ttaacgtgga tattaacata aaaaacattc aataattcat
      181 tcctgcttgt agcccccatc acatgaaata aaataatgat atctaattta ataaaaatac
      241 atttttcaat ttaactttat cgtcattaat ttatctccct gttgaattag cccctctatc
      301 atctacttaa caaatatctc ccatacatgg caacataaaa accgtagatg cactcctttt
      361 ttgtatgtct ggctgcccat tccatccttt caacattcct tcagactgac atttacagtc
      421 tataacgcag gtattatcgg ccactcaata tcaggtgcag ttgatgtatc aacacggttc
      481 agcaacaccc gatacttttt ccaggcttcc agcaacgagg tttcttcctc cgttgcgatt
      541 tccagatctg cagcatcctg aagtggcgcg atatgctcac cggctacctg catcaggctg
      601 ttttttgttc cttccgcctc ccggatccga aacagttttt ctgcttctgc atctttcacc
      661 caagctgtgc cgttccactt gatatttccg atctaaagaa actgtcgcaa aagtcatgga
      721 ctgatataac ccacaaaaaa atgctcataa acgttttcat gcaggcaaat gcgtccacat
      781 caaggcttca acctggtgct ctccacctgt cactggataa acttgcctgt aaaaatcaaa
      841 ctccccctgc tcattaccaa taccaatcag ttgaatgcaa ttgccatcta caataaaatt
      901 ttccgccgaa ggaatcaaaa taaaggcctc cggatgttca ttgggaaagg tgttagcaag
      961 tgtgcgctga atatccctct ccagcaaaac ctcctgagta ttagcataaa gctctggcgt
     1021 aagttcatct atgaaatcgt taccatctct tgggcgcata tgtatcattg aatactgctg
     1081 ctctccagat tctgataatc catggcggac aataagaaca ctacagccgc tttgctgatt
     1141 ggttagccat aacggtgaac tttgctggat agtttgagaa atatcatttc gccggaggct
     1201 tatacactga ttacccaagc ttataactgc aggcgcatca aaacgttccg gactatcagt
     1261 atcacgcata cctgtaaaca gaatgtaatc actaccagcc atatcatctt ccatttcttt
     1321 tagtcttaac aagccatcct ctcttgtaat attaactgta attaaagatg tctcatcatg
     1381 atgggcatag cactgtggat taagaattaa attctcttcc agaaattt
//
LOCUS       DAZFJL010000290         1413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZFJL010000290 DAZFJL010000000
VERSION     DAZFJL010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.293"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            394..657
                     /gene="ykgM"
                     /locus_tag="ACHJ8C_004732"
     CDS             394..657
                     /gene="ykgM"
                     /locus_tag="ACHJ8C_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO5554420.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            657..797
                     /gene="ykgO"
                     /locus_tag="ACHJ8C_004733"
     CDS             657..797
                     /gene="ykgO"
                     /locus_tag="ACHJ8C_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO5554421.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(832..1057)
                     /gene="ykgL"
                     /locus_tag="ACHJ8C_004734"
                     /pseudo
     CDS             complement(832..1057)
                     /gene="ykgL"
                     /locus_tag="ACHJ8C_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
     gene            1204..>1413
                     /locus_tag="ACHJ8C_004735"
     CDS             1204..>1413
                     /locus_tag="ACHJ8C_004735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554422.1"
                     /translation="MQCPGKEAIVPVTTGSLRLTSLNLALRGLTGQMTYLHLFNFSSL
                     IGMNTHNHFVRHVNPLKHLTDWQIIF"
BASE COUNT          405 a          313 c          283 g          412 t
ORIGIN      
        1 acgcctattt atttctccat ttctattaat aacggcaata aactgttcac ttcagtgata
       61 tttaaaatat gcatcctctc ccttttttgt aagtaattat tatatccgtg ggagaggaat
      121 acacattgtc aggtaatcaa tcatgctgca ataaatcatc ggccagtaaa gtggagatag
      181 cctccattct cgaaaaatcc atactctcag cgaaaccatc atcaatcact catccaggcg
      241 tttatgggag cgtcgccaat ggctgctaac aatgccagac ttccccgttg cggaaattcc
      301 acatcccaca aatagtcaca gtgattgggt gttgaaatga tccggatgag catgtatctt
      361 tacggttatg ttataacata acaggtaaaa atgatgaaac ccaatatcca tcctgagtat
      421 cgtactgtgg tgttccacga caccagcgtt gatgagtact ttaaaatcgg ctcgactatc
      481 aaaacagacc gtgagattga gctggatggc gtaacgtatc cgtacgtaac aattgatgtc
      541 tcttcgaaat cgcacccgtt ctatacaggg aagctgagaa cagtggcatc agaaggaaat
      601 gttgcacggt tcacccaacg ttttggtcgt tttgttagca cgaaaaaggg ggcatgatga
      661 aagtccttaa ctctctgcgt accgcaaaag aacgccatcc agactgtcag attgtgaagc
      721 gaaaaggacg gctgtatgtg atttgtaaat ctaatccacg ttttaaggcc gttcagggtc
      781 gtaagaaaaa acgttgattc aaaattcgac gaattaacga tatttgtctg attaataatc
      841 agaccggatt aatgttggtg tatttattac accagcatta attttcctgg ggatgtatct
      901 tcctgttcat ttaaggtcaa ctgcctgacg tttctctccg aatattccat tatattaatg
      961 ttgacttgtt aaccagtttc gccctgtatg ctggcatcaa ctctatttta gactgaacac
     1021 gccactcagt ttcctccctt tgcgacgcag cctgcattta cactcaaact gttaagatga
     1081 taaatgtggt aaatctgttg gtactgacat aaaaacgttt acgccacagg aacggccaga
     1141 tccatcggta accccatcgc cgacgttcga gtgccagtta gagtaacacg cacagatgac
     1201 tgaatgcagt gccctggcaa agaggccatc gttcctgtga caactggcag tcttcgttta
     1261 acttcactta atttggctct tagggggctt accggacaga tgacgtactt acacctgttt
     1321 aatttttcat cacttattgg gatgaacacc cataaccatt ttgtgcggca tgttaatcca
     1381 ttaaaacacc ttactgattg gcaaatcatc ttt
//
LOCUS       DAZFJL010000291         1350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFJL010000291 DAZFJL010000000
VERSION     DAZFJL010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.294"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..1269
                     /locus_tag="ACHJ8C_004736"
     CDS             <1..1269
                     /locus_tag="ACHJ8C_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO5554423.1"
                     /translation="PYISDWHRDLFAVRSKRVKKYLRSSGVFDAFNELVCTLEDAHNA
                     SVSNPKNRIAELCVRGKAVCELSEDMGLSGYFIVLTTPSRFHPTTSFKVAGKWHSRPN
                     KKWWEAGCPTIKDSHAWLNTVWRRVCRRLDKAGIQIPGLRTVEPHADGTTHWNFLIYC
                     NPHESATVLAIFREEAMRDEPDEKGAEEHRIRIEAIDPEKGDGFRYIVKYITKMAGDA
                     SADGIASLNDRYSARSFCDAVSRAACWQKATRLRLFQFFGVPSVTAYRQMRSFRAPLE
                     AHHINMQQFTPQQVAELEAIRMACDAGDFRTYILLNGGFFCSERLLRPFYVQPQEGGK
                     PRINRYGEPCAPVISGFMFGPVPVITRFMGCVVRRMTPAEKIRADEIRSGNSYESVFI
                     SSASRHRTTRSRAAGGGGGADPWTCDNNCP"
BASE COUNT          273 a          375 c          368 g          334 t
ORIGIN      
        1 ccttatattt ccgactggca ccgcgacctt tttgctgtcc gcagtaaacg cgtgaaaaag
       61 tatctccggt cctccggcgt ctttgatgcg tttaatgagc tggtctgtac gctggaagat
      121 gcgcataacg cctctgtttc gaacccgaaa aaccggattg ctgaactctg cgttcgtggg
      181 aaggccgtct gtgaactgtc cgaagatatg gggttgtccg gttacttcat cgtgctcacc
      241 acgccgtcac gttttcaccc gacgaccagt tttaaggttg ccggaaaatg gcattcccgc
      301 ccgaacaaaa aatggtggga agcaggttgc ccgaccatta aggattcgca cgcctggctg
      361 aataccgtct ggcggcgggt ctgccgcaga ctggataaag ccggcattca gatacccggt
      421 ctgcgcacgg ttgagccgca tgctgatggt acaacgcact ggaatttcct gatttactgc
      481 aatccccatg agagcgcaac ggtgctggcc attttccgtg aagaggccat gcgcgatgag
      541 ccggatgaaa agggggcgga agagcaccgt atccgtattg aggctattga ccctgaaaag
      601 ggcgacggtt tccgttacat cgtgaagtac atcaccaaaa tggcgggcga tgccagtgcg
      661 gacggtattg cctccctgaa tgaccgttat tctgcccgtt cattctgtga tgcggtcagc
      721 cgtgccgcct gctggcagaa agcaacgcgt cttcgccttt tccagttctt tggcgttccg
      781 tctgtcacgg cttaccgcca gatgcgcagc ttccgcgcac cgcttgaggc gcatcatatc
      841 aatatgcagc agttcacccc gcagcaggtc gctgaacttg aagccatccg catggcctgt
      901 gatgcaggcg atttccggac ctatatcctg ctgaacggcg gtttcttctg ttctgaacgt
      961 ctgcttcgtc cgttttacgt tcagccgcag gaaggtggta agccccgtat taaccgttac
     1021 ggcgaaccct gcgcgccggt gatttccggt ttcatgtttg gccccgttcc ggtcataacc
     1081 cgttttatgg ggtgcgttgt ccgccgtatg acccccgcag agaaaatccg cgcagatgaa
     1141 atcagaagtg gcaacagcta tgaatctgtg ttcatttcgt cggcttcacg acaccgcacg
     1201 acgcgctccc gcgcggcggg cgggggcggc ggagccgacc cttggacttg tgacaataac
     1261 tgtccctgac tgaattttta acattgagga gtctgattta tgaatcaaat ttacgacagc
     1321 tttccgtttc cttcccctga tggttttacc
//
LOCUS       DAZFJL010000292         1349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZFJL010000292 DAZFJL010000000
VERSION     DAZFJL010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.295"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..170)
                     /locus_tag="ACHJ8C_004737"
     CDS             complement(<1..170)
                     /locus_tag="ACHJ8C_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO5554424.1"
                     /translation="MSRVTAIISALVICIILCLSWAVNHYRDNAIAYKDQRDKATSII
                     ADMQKRQRDVAEL"
     gene            complement(167..664)
                     /gene="rrrD"
                     /locus_tag="ACHJ8C_004738"
     CDS             complement(167..664)
                     /gene="rrrD"
                     /locus_tag="ACHJ8C_004738"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO5554425.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRRDIEREVC
                     LWGQQ"
     gene            complement(664..879)
                     /locus_tag="ACHJ8C_004739"
     CDS             complement(664..879)
                     /locus_tag="ACHJ8C_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5554426.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            1022..>1349
                     /locus_tag="ACHJ8C_004740"
     CDS             1022..>1349
                     /locus_tag="ACHJ8C_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554427.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTMLSIST
                     QYVVLLVTNIITINRHRISPVYRNRVKKPEPESNHRMTICQWQVIKKRPRHAQPELTR
                     KMIEGNN"
BASE COUNT          349 a          350 c          294 g          356 t
ORIGIN      
        1 agttctgcta catcacgttg acgcttctgc atgtcagcga tgatggatgt ggctttgtcg
       61 cgctggtctt tgtaggcgat ggcgttatca cggtaatgat taacagccca tgacaggcag
      121 aggatgatgc aaataaccag agcggagata atcgcggtta ccctgctcat tgctgccccc
      181 acaaacagac ttcacgctca atatcacgac gggtcatcag ccctttccat tgcttaccgc
      241 cagcgtatgt ccagcgacgt agctggtcac atgcgccttt gatatcaccc tggtttattt
      301 tgcgaagaag cgtcgatgtt ctgaaattac cagcgcccac gttgtaaacg aacgagtaaa
      361 gagcgccgcg cgttgtttcc ggtatatcga ctttgatgta cgggttaatt tgtctggcga
      421 ccgtggcaag gtctttattc aggagggctt tgcattctgc ttcggtatac gttttaccgg
      481 gaatgatgtc ttttccggtg tgtccgtgac atacagtcca tacgccaacg atatcttcgt
      541 atggtatgta gctgacacct tccaggccat cgtcaccact cggaccagtg atgagcacag
      601 acgctatggc aacagcccca ccaccaatag cagcagcaac agccttgcgt aatgatggcg
      661 acattattca cctctcgcag ccttacgctt atcttcttta atcttgaaat aaaggtttgt
      721 cagatacgtc agcaagccaa acaccaggct acccaatacg cctattgcca cccactggga
      781 tggggagact ttgtccagca actgcagtag ccagtatccc gtccccaccg ctgacgtggt
      841 gtatgacaca cctgttgtta ttttttccat ctggtacata ccccgtctcc cgttatccgg
      901 aagctgacaa caataaaaag ccaccagtta attcctgatg gccctgatgc ataaacgtca
      961 taatacctga ctgttatgat tgacaataat gataatgttt atatagaaag gttcccgatg
     1021 tgtgttacat atcatttctc cacggggaat atccccacgc cagcgcagac tcttttaccc
     1081 gttctcttct gcgctggctc ttttttatta tgctgctgca tttacctctg gcaccatgct
     1141 ttctatctca acacaatacg tggtacttct tgtaaccaat atcataacga ttaatcgaca
     1201 tagaatttct cccgtgtaca ggaacagagt taaaaagccg gaaccggaat caaatcacag
     1261 gatgaccatc tgccagtggc aggtcataaa aaaaaggccg cgccatgcgc agccagaact
     1321 cacaaggaaa atgatagaag gaaataaca
//
LOCUS       DAZFJL010000293         1348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFJL010000293 DAZFJL010000000
VERSION     DAZFJL010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.296"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..287)
                     /locus_tag="ACHJ8C_004741"
     CDS             complement(<1..287)
                     /locus_tag="ACHJ8C_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554428.1"
                     /translation="MATLRELIIKISANSRSFQSEISRASRMGQDYYRTMQNGGRQSA
                     AASREMRRALAEVTDQINTAKSSALNMAGAFAGAFATGHLISLADEWNSVNA"
     gene            complement(334..612)
                     /locus_tag="ACHJ8C_004742"
     CDS             complement(334..612)
                     /locus_tag="ACHJ8C_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004967194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO5554429.1"
                     /translation="MMLALRMGRTLSELRREMSASEIMMWAEFDRFSPLGDERADIRA
                     AQIVSAVYGAQGVKVPLNDALLQWEKEQTEGVSDPFAGLENALLIVSQ"
     gene            complement(636..1007)
                     /locus_tag="ACHJ8C_004743"
     CDS             complement(636..1007)
                     /locus_tag="ACHJ8C_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO5554430.1"
                     /translation="MTKNIRNLALATMSGFRHKTVDVPEWEGATVVLREPSAEAWLRW
                     QEIVKAKDDETPLSVAERARRNLEADVELFIDVLCDTGLQPVFSEDDREQVIAVYGPV
                     HARLLRQSLELISDAGEVKKK"
     gene            complement(1022..>1348)
                     /locus_tag="ACHJ8C_004744"
     CDS             complement(1022..>1348)
                     /locus_tag="ACHJ8C_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HFO5554431.1"
                     /translation="VVAATFSLRLKGKPVLTTEPLKVKVDLNSTLQVSAGAKLEMVVE
                     AAGGVPPYSYVWKKGGSPVSGQTAATFSKASAASGDAGAYTCEISDSASPVSKVTSTS
                     CTVTVS"
BASE COUNT          334 a          403 c          299 g          312 t
ORIGIN      
        1 gcatttactg aattccactc atcggcgaga gaaataagat gaccggtagc aaaagccccg
       61 gcaaatgccc ccgccatatt cagtgccgaa gatttagctg tatttatctg atccgtcact
      121 tctgccagtg cacgccgcat ttcacgggat gcagcagcgg actgccggcc tccgttctgc
      181 atggtacggt agtaatcctg ccccatacgc gaagcccggg agatctctga ctggaatgac
      241 cgggaatttg ccgagatttt aataatcagt tcacgtaatg tcgccacact cattctccgg
      301 acgaaaaaaa accgccgaag cggttatgtt gactcactga gacactatta aaagcgcgtt
      361 ttccagtccg gcaaatggat ctgatacgcc ttctgtctgc tccttctccc actgaagaag
      421 cgcatcattc agtggtactt tgacaccctg cgcaccgtaa acagctgaaa ctatctgggc
      481 agcccggata tcagcccgtt cgtcccccag cgggctgaac ctgtcaaatt ctgcccacat
      541 catgatttct gatgcggaca tttcccggcg taactctgac aatgtgcgcc ccatcctgag
      601 cgccagcatc atcagaaaac gcatccccgg aagcgctact tttttttaac ctcaccggca
      661 tcactgatca gttccagaga ctgccgaaga agccgcgcat gcaccgggcc atacacggca
      721 atcacctgtt cacgatcatc ctctgaaaat acaggttgca gtccggtatc acacagaaca
      781 tcaatgaaca gttcaacatc tgcctccaga tttcggcggg cgcgctccgc aacagataac
      841 ggtgtctcat catcttttgc tttgacgatc tcctgccagc gcaaccaggc ttctgcagaa
      901 ggttcccgta atacaaccgt tgccccttcc cattcaggca catcaacagt tttatggcga
      961 aaccccgaca tcgttgccag tgccagatta cggatatttt tagtcatcac atctatcctc
     1021 attaactgac ggtaacagtg caggaagtgg aggtcacctt gctaaccggg cttgctgaat
     1081 cagaaatctc gcaggtatac gcaccggcat caccggatgc tgctgatgcc ttactgaacg
     1141 ttgccgccgt ctgtccggaa acaggagaac cacctttctt ccagacataa gaataaggcg
     1201 gcacaccacc ggcagcctca accaccattt cgagtttcgc tccggcagaa acctgcagcg
     1261 tgctgtttaa atcgaccttc actttcagcg gctctgtcgt cagtacaggt ttacctttca
     1321 ggcgcaggga aaacgttgca gccacaac
//
LOCUS       DAZFJL010000294         1332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFJL010000294 DAZFJL010000000
VERSION     DAZFJL010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.297"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            209..297
                     /locus_tag="ACHJ8C_004745"
                     /pseudo
     CDS             209..297
                     /locus_tag="ACHJ8C_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(713..1282)
                     /gene="nleF"
                     /locus_tag="ACHJ8C_004746"
     CDS             complement(713..1282)
                     /gene="nleF"
                     /locus_tag="ACHJ8C_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
                     /protein_id="HFO5554432.1"
                     /translation="MLPTSGSSANLYSWMYVSGRGNPSTPESVSELNHNHFLTPELQD
                     KLDVMISIYSNARNSNELEEIYQELSAFVSGLMDKRNSVFEVRNENTDEVVGALREGM
                     TIDDRDGYIRELFFLYSLKVKIEESRQGKEGSKCKVYGLLCPHHSSELYGDLRAMKCL
                     VEGCSDDFDPFDIIRVPDLTYNKGALQCG"
BASE COUNT          424 a          299 c          207 g          402 t
ORIGIN      
        1 tgataccgaa tcgcaaaatc aaaaggttat aaatttttct tggaataaat gtcttaaaaa
       61 catacacgaa aaatcaataa actacgttga attcaataag aatattccaa gcattctttg
      121 agttattttt gaccagtttg ctgtttttct ttctcataaa atatttcctg atatccctta
      181 acattactga tactcaagta gtgaatttat gagcaatcct aatccatgca tgacgtgtgg
      241 tgcctgttgt gcatttttcg cgtctctttt tactgggctg aagcagatga tgctggcgaa
      301 aggtttatca gaaaaccgta gcgccttctt gcatacgacg tgtccgcagt tttatataat
      361 cccgaccaga aacctcttat aaaaagtcga tacacaaata tcatggataa tcaccacctt
      421 ctgacgaaga atatctgatg aaattaatcg cccctctcct gagaccattg cgctggggta
      481 aatttgtaac tacgtgtgta cagtgaggtg tcgtatagca cgccgtcagg aaatgcaagg
      541 tgcccccgat atccccgact acaagtcagc aaacaaagtg attggctggc aatccacatc
      601 acagaaatgg acaatgctac tttataatcg agcttggtag ccaccttgat ggtcactttt
      661 aaaaaaagcg tttacacaaa atgataagca ggctcacagg acctccctct aatcatccac
      721 attgtaaagc tcctttgttg taagtaagat ccggcaccct aataatatca aaaggatcaa
      781 aatcatcact gcatccttcc acaagacatt tcattgctcg tagatcacca tatagctctg
      841 aagagtgatg cggacataac agaccataaa ctttacattt agaaccttct ttaccttgtc
      901 tactttcctc aatttttact ttcaatgaat acagaaaaaa aagctccctg atataaccat
      961 ccctgtcatc tatcgtcatc ccctccctca gtgctccgac aacctcatca gtattttcat
     1021 ttctcacctc aaatacacta tttctcttat ccatcagccc acttacaaaa gcacttagct
     1081 cttgataaat ttcctcaagc tcattactat ttctggcatt tgagtatata gagatcataa
     1141 catcaagttt atcttgtaat tcaggagtaa gaaaatgatt atgattaagt tcacttactg
     1201 attccggagt cgaagggtta cctcttcctg atacatacat ccatgaataa agatttgctg
     1261 aagagccact tgttggtaac atatcaaccc ccccttaaca aataaatatt ataaaaaata
     1321 tggctgtata aa
//
LOCUS       DAZFJL010000295         1316 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZFJL010000295 DAZFJL010000000
VERSION     DAZFJL010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.298"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            254..>1316
                     /locus_tag="ACHJ8C_004747"
     CDS             254..>1316
                     /locus_tag="ACHJ8C_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HFO5554433.1"
                     /translation="MTNLLTALVFLTSACVHASSPAFIVDRLPLNAAIQLAQEDVFGR
                     QYVLPPELASDTRPVTLDLSLAGDQKTQREEYVRWLRQMNIAVETRNGVDHYRSFKPV
                     AAPEKMVSWVYTPLHRSPSYLATVLSGTTGRSSQASSESTSGTSSGSLSGSVSSSSGS
                     FLSGEGDSLVFRGTRSELARLKELVPLIDVPAQGVVVTGYIYEVQTGRSEGSGLALAA
                     KLLSGRFGVSVGSSSSMGNYISFSSGTLNAMYELFSTDNRFKVVSAPQLRMDSGKEAT
                     FSVGEQVPVLGSVSYEDGKAVQSVTYRDSGVIFKVKPVITSSRISLNVNQQLSNFVKT
                     DTGVNDSPTLLKREVDTSLT"
BASE COUNT          302 a          293 c          394 g          327 t
ORIGIN      
        1 tcatggaacg gcggcgcgac tgaactgaat gttgaaggtg aacgggtgac gttctggtca
       61 ggcccgtcgg gaacggcatc aaagggtgat gtactgactg acaaaatgct ttcgtttgat
      121 gtgaagagta aataagagag agccgccacc gctcgccgca ggtttatgac cgaacgtagt
      181 gagcgaataa acgaggaagc ggaagagaac ggaacagaca taaggcactt acgcacggta
      241 taacatgatg agaatgacta acttactgac agcacttgtt tttctgactt cggcatgtgt
      301 tcatgcctcg tctccggcat ttattgttga ccgccttcca ctgaacgcgg caatacagct
      361 tgcacaggag gatgtgtttg gacggcagta tgtgctgcca ccggaacttg ccagtgatac
      421 acgcccggtc acactggatt taagtcttgc aggtgaccag aagacccagc gtgaggagta
      481 tgtacgctgg ctccggcaga tgaatattgc ggtggaaaca cgaaacggcg ttgaccatta
      541 ccgctcgttt aaacccgtgg ctgcgccgga aaaaatggtg tcatgggtat atacgccgct
      601 acatcgatcg ccgtcctatc ttgccacggt actttcggga acaacagggc gctcatcgca
      661 ggcatcttct gagagtacgt ccggtacgtc ctcggggtcg ttatccggca gtgtatcgtc
      721 atcgtcaggt tcatttttat cgggtgaggg tgattcactg gttttcaggg ggacacgttc
      781 agagctggcg cgtcttaagg agctggtccc gctgattgat gttccggcgc agggtgttgt
      841 ggtgacgggg tatatttatg aggtacagac agggcgttct gaaggttcgg ggctggcact
      901 ggccgcaaaa ctgttatcag gtcgttttgg ggtcagcgtg ggttcctcgt catcgatggg
      961 taattatatt tcgttcagct ccggtacgct gaatgcgatg tatgagctgt tcagtacgga
     1021 taaccgcttt aaggtggtat cagcacctca gttacgcatg gattcaggca aggaggcgac
     1081 gttctccgtt ggtgaacagg ttccggttct tggcagcgtg tcctatgagg atggtaaagc
     1141 cgttcagtcg gtgacgtatc gtgattcggg ggtgatattc aaagtgaagc cggttatcac
     1201 atcgtcacgc atcagtctga acgtcaatca gcagttaagt aactttgtca aaacggatac
     1261 gggcgtgaat gattcaccaa cgttgctcaa gcgtgaggtg gatacgtcgc tgaccc
//
LOCUS       DAZFJL010000296         1312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFJL010000296 DAZFJL010000000
VERSION     DAZFJL010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.299"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..835)
                     /locus_tag="ACHJ8C_004748"
     CDS             complement(<1..835)
                     /locus_tag="ACHJ8C_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO5554434.1"
                     /translation="MKRIPPHERSYAEVLTKCSEGMEQRNVKANFIEQFDSLLVKETE
                     YRNLCILGHLFRYQKTEPLHDNTVVIGNLTKSKLINLYENNLRNKDKPARVYYDELLV
                     SSGEKCPFCGDIGQTKNLDHFLPKAHFPEFSIMPLNLVPSCRDCNMGEKGEGYAINEA
                     EQSLHPFIDKDIFFQEQWVFADYINEDDGAISYYVQCPLNWRQEDKDRAFNHFNNLNL
                     AARYQLEAGKHLSEVIDQRDSFATTLKQYVPTATPEMIRDAFISATLQPIINSGQFHN
                     HW"
     gene            complement(832..>1312)
                     /locus_tag="ACHJ8C_004749"
     CDS             complement(832..>1312)
                     /locus_tag="ACHJ8C_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5554435.1"
                     /translation="GHAIVLFTITKLVDTVGEKSLVLLDEPEGHLHPPLLAAFLRSLS
                     DLLYAQNGVAIIATHSPVVLQEIPKSNVWKILRSREATNITRPDIETFGENLGVLTRE
                     VFSLEIMSSGYHALLAKSVASGLSYDDILAQYKNKIGLEGRTVLKAMVLSRDAGDKK"
BASE COUNT          384 a          267 c          238 g          423 t
ORIGIN      
        1 tccaatggtt gtgaaattga ccagaattta taattggttg taaagttgcg ctaataaagg
       61 catctcttat catttcaggg gttgctgttg gaacatattg cttaagtgtc gttgcgaatg
      121 aatccctttg atctataact tcactgagat gctttcctgc ttctaactga tatcttgcgg
      181 ccagattgag attgttaaaa tgattaaagg cacgatcctt atcctcttgt ctccaattga
      241 gtgggcattg aacataataa cttatcgctc catcatcttc attaatatag tcagcaaaaa
      301 cccactgttc ttgaaagaaa atgtccttat caatgaatgg atgaagtgac tgctcagctt
      361 catttattgc atacccttcc cccttttccc ccatattgca atcccggcaa gagggcacta
      421 aattaagagg cataattgaa aattcgggaa aatgagcttt aggtaaaaag tgatcaaggt
      481 ttttagtttg cccaatatct ccacaaaatg gacatttttc accagatgaa acaagaagtt
      541 catcatagta aactctcgca ggcttatctt tgtttctgag attgttttca taaaggttga
      601 taagttttga cttcgttaga ttacctatga caactgtatt atcatgaagt ggttctgttt
      661 tctgatatct aaataaatgt cccaatatac acaaattacg gtattcagtt tctttgacca
      721 gtaatgaatc gaattgttcg ataaaattgg ccttaacatt ccgctgttcc atgccctcag
      781 aacactttgt gaggacttcg gcataagatc tctcatgagg tgggattctt ttcatttttt
      841 atcccctgca tcacgactca gaaccattgc ttttaaaact gtcctaccct ccaagccaat
      901 cttgttttta tattgtgcta gtatatcgtc ataagataga ccactggcca cagactttgc
      961 tagcaacgca tgatatccag aactcataat ttctaaagaa aaaacctctc tagtcagtac
     1021 ccctaagttc tcaccaaaag tttcgatgtc tggcctagta atatttgttg cctcacgaga
     1081 gcgtaatatt ttccaaacat tggacttggg aatctcttgt agaaccactg gtgagtgtgt
     1141 agcgataata gccactccat tttgtgcata taataaatcg cttaatgagc gtaaaaatgc
     1201 cgcgagcaac ggtggatgaa gatgcccttc gggttcatcg agcaacacaa gtgatttttc
     1261 accgactgtg tccactaatt ttgtaatggt aaataaaacg atagcatggc ca
//
LOCUS       DAZFJL010000297         1291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZFJL010000297 DAZFJL010000000
VERSION     DAZFJL010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.300"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..626)
                     /locus_tag="ACHJ8C_004750"
     CDS             complement(<1..626)
                     /locus_tag="ACHJ8C_004750"
                     /inference="COORDINATES: protein motif:HMM:NF017195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554436.1"
                     /translation="MAYIFIFLVAVFSFDSAYAASMTEYLGDSGSGTRVARAGVAPPC
                     TTGYASCSGDTFYCTTSYSDTKQLSGMPFLLVTGNWISYKSYHFEDGVKVYSQCKDSY
                     YGQYFGASYSAKPTEEVCLSRPEISNTSLSGVYMDDKGNKYVTIHGCIYEATGDVISC
                     SNDSTHCSATWKPLTVDPSYSDKEDTEDKGDSGDTDDKGNNGNTDGGD"
     gene            complement(686..928)
                     /locus_tag="ACHJ8C_004751"
     CDS             complement(686..928)
                     /locus_tag="ACHJ8C_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554437.1"
                     /translation="MKVKTLVLSGLTTAGIVMSSSASAAVTVPSFITPDSVGEIGTAI
                     TSVIGIAGTAAFAVLGVSLAARLGIGIIKGFLSRAT"
     gene            complement(954..1124)
                     /locus_tag="ACHJ8C_004752"
     CDS             complement(954..1124)
                     /locus_tag="ACHJ8C_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554438.1"
                     /translation="MTEFDFSGVLSAINSGVQDGELYWAVLYVWIVCWLLGFGLGVIF
                     TLVCRMFSDVVN"
BASE COUNT          452 a          299 c          246 g          294 t
ORIGIN      
        1 ttatcaccac cgtcagtatt accgttattc cctttatcgt cagtatctcc gctatcacct
       61 ttatcttctg tatcttcttt atcagaataa gacggatcaa cagttaaagg tttccaggtt
      121 gcggaacaat gggttgaatc attggaacaa gaaataacgt cacccgttgc ctcataaata
      181 catccatgaa tggtgacata tttatttcct ttatcatcca tataaacacc tgacaaagaa
      241 gtattactaa tctcaggacg ggataaacaa acctcctcag taggtttagc tgaatacgaa
      301 gcaccaaaat attgaccata atagctatcc ttacactgag aataaacctt aacaccatcc
      361 tcaaaatggt aagacttata agagatccag ttacctgtaa ccagtaaaaa aggcatacca
      421 gaaagttgct tagtatcgct gtaagacgtc gtacaataaa acgtatcacc tgagcaggat
      481 gcatatccag tagtacaggg tggagctaca ccggcccttg caacacgggt accggaacct
      541 gaatcaccaa gatattctgt cattgaagct gcatatgcag aatcaaatga aaaaacagct
      601 acgaggaaaa taaatatata tgccaagact caccccctga ataagcaaaa aaagcgcccc
      661 gcagggcgct gttgttaaat aactatcagg ttgcgcggga aaggaagcct ttaatgatac
      721 cgatacccag acgggcagcg agtgaaacac caagaacggc aaatgcagcc gtacctgcaa
      781 tgccaataac actggtaatg gctgtgccaa tttcaccaac cgaatcaggg gtaataaagg
      841 aaggaacagt aaccgcagca gaagccgaag acgacattac aatacctgcc gtggtcagtc
      901 ccgataatac cagggttttt actttcataa aattcctctt tgacgattaa aaattaatta
      961 acaacatcgc tgaacatccg gcagaccaga gtgaaaataa caccaaggcc aaaaccaaga
     1021 agccagcaga caatccagac atacagaacg gcccagtaaa gctccccgtc ctgtacacca
     1081 gaatttattg cggataaaac accgctgaaa tcgaattcag tcataataac gctgaacagt
     1141 aatgatttgt gagtcacagg cgcgaatcag ttttattccg atgccaaaac ctgaagagaa
     1201 taaaatcatt gcaggcgcag cgaataaaac aaagttaaga aaatcagcgt cagacatatc
     1261 atcagccttt ttctttacct gcgggcagtg c
//
LOCUS       DAZFJL010000298         1261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFJL010000298 DAZFJL010000000
VERSION     DAZFJL010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.301"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            61..270
                     /locus_tag="ACHJ8C_004753"
     CDS             61..270
                     /locus_tag="ACHJ8C_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020314678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554439.1"
                     /translation="MKVLIGNINIDNYHMLSALAGIAGFDRSIQFTCEISASIEIMED
                     DFVNKAGILKMLDEFIENDFSIKLV"
     gene            368..592
                     /locus_tag="ACHJ8C_004754"
     CDS             368..592
                     /locus_tag="ACHJ8C_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554440.1"
                     /translation="MKQSTFPVIVSTTGHVFSVVRVTLCTICLKHEKTGEAYVVIFTD
                     CHNIRDYKKGVVPVLGELYQEDVDLITGKS"
     gene            644..904
                     /locus_tag="ACHJ8C_004755"
     CDS             644..904
                     /locus_tag="ACHJ8C_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554441.1"
                     /translation="MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTV
                     ADLVFHEEAFDTIDFASDGLRENDKEIVSMMIQEMSEGKNAK"
     gene            894..1145
                     /locus_tag="ACHJ8C_004756"
     CDS             894..1145
                     /locus_tag="ACHJ8C_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5554442.1"
                     /translation="MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGT
                     ASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDSGSN"
BASE COUNT          431 a          194 c          277 g          359 t
ORIGIN      
        1 gacttgcaac cagattgtaa tttttaatat ggggggattc cccccatatc aggaggataa
       61 atgaaagttc ttattggtaa tataaatatt gataattatc atatgctgtc tgcattagct
      121 gggattgcgg gatttgacag gagcattcag tttacatgtg aaatatcagc aagtatcgaa
      181 attatggagg atgatttcgt aaataaagct ggtatattaa agatgttaga tgaatttatt
      241 gaaaatgatt tttcaataaa gctcgtttag aaaatacttg ataataggtc atttatgacc
      301 tattattaat gcattgagag gggatggtcc ccaatcgcca aagccgctca gcggcaggag
      361 attaaatatg aaacaatcta cttttcctgt tatcgtctct acaactggtc atgtgttttc
      421 tgttgtgcgc gtgacgcttt gcacaatttg ccttaaacat gaaaaaacag gcgaggctta
      481 tgtcgtcatt tttacagact gccataatat ccgtgattat aaaaagggcg ttgttccggt
      541 tcttggggag ctttatcagg aagatgtcga tttgattacc ggaaaaagtt aaaagaaagg
      601 ggctatatgc ccctttactg ttaaaagggg agactatctt gatatgaaga taagaaaagg
      661 tgacagacaa tattatctga ataaagaagg tgatacgttt catctggtga agcgagtaaa
      721 aactttttct aagtctgcga ctttaggaaa aacgaaagca acggtgaaaa ctgttgctga
      781 tcttgttttt catgaggagg cattcgacac tatcgacttt gccagcgacg gattaagaga
      841 gaatgataaa gaaattgttt ctatgatgat tcaggaaatg agtgagggaa aaaatgccaa
      901 atgaaaacac accagaaaac attaaacgtc tgcggcaaaa aattggcctg acacagaaag
      961 aatgtgctga aatattcagc atgagtccgc gtacctggcg tcgcaaagaa gaacctgtcg
     1021 gaactgcaag cggtacagca cttacaccag tagagtttaa atttcttctg cttttagctg
     1081 gagaacaccc tgattatgta ctttgtaaaa ggaataaaaa gaattctgat tctggaagta
     1141 attaaggaat attaagatga aaatagaatt aacgtatgac gaaatcacgg aataagctac
     1201 aaaaaacata actgaattta tggctggact taatagcccc agttgcattg aacaatgagc
     1261 a
//
LOCUS       DAZFJL010000299         1260 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZFJL010000299 DAZFJL010000000
VERSION     DAZFJL010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.302"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            41..1186
                     /gene="espG"
                     /locus_tag="ACHJ8C_004757"
     CDS             41..1186
                     /gene="espG"
                     /locus_tag="ACHJ8C_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspG"
                     /protein_id="HFO5554443.1"
                     /translation="MINGLNNDSASLVLDAAMKVNSGFKKSWDEMSCAEKLLKVLSFG
                     LWNPTYSRSERQSFQELLTVLEPVYPLPNELGRVSARFSDGSSLRISVTNSESIEAEI
                     RTPDNEKITVLLESNEQNRLLQSLPIDRHMPYIQVHRALSEMDLTDTTSMRNLLGFTS
                     KLSTTLIPHNAQTDPLSGPTPFSSIFMDTCRGLGNAKLSLNGVDIPANAQMLLRDALG
                     LKDTHSSPSRNVIDHGISRHDAEQIARESSGSDNQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALTLTHERISKASEYNAERSLDTPNACINISISQSSDGNIYVTSHTGVLIMAP
                     EDRPNEMGMLTNRTSYEVPQGVKCTIDEMVRALQPRYAASETYLQNT"
BASE COUNT          408 a          272 c          238 g          342 t
ORIGIN      
        1 tgccaaattg ttcattacaa accagatagg agaatctctc atgataaatg gacttaataa
       61 tgactccgca tctttagttt tagatgctgc aatgaaagtt aattctgggt ttaaaaaaag
      121 ctgggatgag atgtcatgcg ctgaaaagtt acttaaagta cttagttttg gtttatggaa
      181 tccaacgtac agtcgtagtg aaagacaatc atttcaagag ttgttaaccg ttttagagcc
      241 tgtatatcca cttcccaatg aattaggcag agtatctgct cgtttttcag atggctcatc
      301 cttaagaatt tccgtcacta acagcgaaag tattgaagcc gagattcgca caccagataa
      361 tgaaaagatt actgtgctcc tggagtcaaa cgaacaaaat aggttattac aatctttacc
      421 catcgatcgc cacatgccat acattcaggt tcatcgtgcc ttatctgaga tggacctgac
      481 tgatactacc tcaatgcgca atctacttgg ttttacgtca aaactatcaa caaccttgat
      541 tcctcataat gctcaaacag atccgctttc cgggcctaca ccattcagct ctatctttat
      601 ggatacatgt cgaggactcg gcaatgcaaa gctttcactc aatggtgttg atatacctgc
      661 aaatgcacaa atgttgcttc gcgatgcact aggacttaaa gacacacact catcaccttc
      721 ccggaatgtt atagatcatg gtatttctcg ccatgatgca gagcaaatag caagagaaag
      781 cagcggcagc gataatcaga aagctgaagt tgtggaattt ttatgccatc cagaagcagc
      841 aacggccata tgctcggctt tctatcaatc tttcaatgtg ccagccttaa cgttgacaca
      901 tgaaaggatc tctaaagcca gtgaatacaa tgcggaaaga tcgttagata cacctaacgc
      961 ttgcattaac atcagtatct ctcaatcatc agatggaaac atttatgtta ccagccatac
     1021 cggggttctg ataatggcgc cagaagaccg ccccaacgag atgggcatgt tgacgaacag
     1081 gacttcttat gaagtgccgc aaggtgtgaa atgtacaatc gatgaaatgg taagagcgct
     1141 acaaccaagg tatgccgcat ctgaaacgta cttacaaaac acttaaaata aaactttatt
     1201 aatcaaaacc aataatagaa atcacaccca caattattaa caatttatcc tgaagttaat
//
LOCUS       DAZFJL010000300         1237 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZFJL010000300 DAZFJL010000000
VERSION     DAZFJL010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.303"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            65..484
                     /locus_tag="ACHJ8C_004758"
     CDS             65..484
                     /locus_tag="ACHJ8C_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5554444.1"
                     /translation="MELQDWRKEPRKKYSNEFKLRMIELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRISRRLPVTTTSDAGLELLPVEITPDEPKEPMAALTPPLSTQTT
                     VSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            481..831
                     /gene="tnpB"
                     /locus_tag="ACHJ8C_004759"
     CDS             481..831
                     /gene="tnpB"
                     /locus_tag="ACHJ8C_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5554445.1"
                     /translation="MISLPSGTRIWLVAGITDMRKSFNGLGEQVQHVLNVNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            862..>1237
                     /locus_tag="ACHJ8C_004760"
     CDS             862..>1237
                     /locus_tag="ACHJ8C_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5554446.1"
                     /translation="MSQKYLIRIAELESQLRQKDQQLSLVEETAAFLRSALARAEEKI
                     EEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQDSDRCSGREDDPQ
                     VPRQLRQSRHRRPLPAHLPREIH"
BASE COUNT          325 a          317 c          317 g          278 t
ORIGIN      
        1 tatgtagcca ttagacaaga attggtaact tagacgccca tctgacacag acggacatct
       61 aagtatggaa ttacaggact ggcgaaaaga acctcgtaaa aagtattcga atgaattcaa
      121 acttcgtatg attgaactgg catcacaacc cggtgcttgt gtggctcaga ttgcacgaga
      181 aaatggcgtc aatgataatg ttattttcaa atggctcaga ctctggcaaa acgaagggcg
      241 tatatcacgg cgtcttccgg tgacaaccac ttctgacgca ggccttgaat tattacctgt
      301 agagataacg cctgatgagc cgaaagaacc gatggctgct cttactccgc ccttatccac
      361 tcagactact gtcagtgcca gctcctgcaa ggtggagttc cgtcacggta acatgacgct
      421 ggaaaatcct tcaccagagc tgctcactgt attgattcgt gaactgaccg ggaggggaag
      481 atgatctcac tcccatccgg tacccgtatc tggctcgttg ccgggataac cgatatgcgt
      541 aagtctttca acgggctggg tgaacaggta cagcatgtgt taaatgttaa tcccttctcc
      601 ggtcacctgt ttatcttccg tggccgacgg ggtgacaccg ttaaaattct ttgggctgat
      661 gctgatggtc tgtgcctgtt caccaaacgt ctggaggaag gccagtttat ctggcctgcg
      721 gtgcgtgacg gtaaggtatc cattacccgc tcgcaactgg caatgctcct cgataagctg
      781 gactggcgtc agccaaaaac atcccgcctt aacgccctga caatgttgta aaaaacgcct
      841 ggccgcatta taaaaacggc catgagtcag aaatacctca ttcgcattgc agaactggaa
      901 agccagctcc gtcagaaaga ccagcaactg agtctggttg aagagacggc ggccttcctg
      961 cgctctgcac tggcacgtgc cgaagaaaag atcgaagagg atgaacggga aatagaacat
     1021 ctgcgggctc agatagaaaa actgcgccgg atgctgttcg gtacccgttc tgaaaaactg
     1081 cgtcgtgaag ttgaacaggc tgaggccctg ctgaaacaac gcgaacagga cagcgatcgt
     1141 tgcagtgggc gggaagacga tccacaggtt ccccgccagt tgcgacagtc gcgccatcgt
     1201 cgtccgttac cggcacacct tccccgtgaa atacacc
//
LOCUS       DAZFJL010000301         1223 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFJL010000301 DAZFJL010000000
VERSION     DAZFJL010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.304"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            161..763
                     /gene="ais"
                     /locus_tag="ACHJ8C_004761"
     CDS             161..763
                     /gene="ais"
                     /locus_tag="ACHJ8C_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO5554447.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRFLQCGNEIYSAIKDLQRKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(802..>1223)
                     /gene="nudI"
                     /locus_tag="ACHJ8C_004762"
     CDS             complement(802..>1223)
                     /gene="nudI"
                     /locus_tag="ACHJ8C_004762"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO5554448.1"
                     /translation="QRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGERI
                     EEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSANRD
                     VKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          326 a          276 c          257 g          364 t
ORIGIN      
        1 tatatttttt cttatttaga ctttgcattt ggcaaaattt tgaggcattt ttccggcatc
       61 ataggatttt taatattaca ccaactgcta attatcgcca gaaaagtcgc tcaatttcac
      121 ggtaattgtc tggttgcgct tgtctatagg tggagtttac gtgttagctt tttgccgatc
      181 ttcgttgaag tcaaaaaaat atttcatcat tttactggcg ctcgctgcga ttgccggact
      241 gggtactcat gccgcctgga gtagcaatgg tttgccacgc atcgacaata aaacactggc
      301 cagactggca cagcagcacc cggttgtcgt tttgtttcgc catgctgaac gttgcgatcg
      361 ttcaaccaat caatgcttgt cagataaaac aggtattacg gtcaaaggta cccaggatgc
      421 ccgtgaactg ggcaacgctt ttagtgctga tatccctgat tttgatctct attccagtaa
      481 taccgtccgg actattcagt cggcgacctg gttttcggcg ggtaaaaagc tgacggtaga
      541 taaacgattt cttcaatgcg gtaatgagat ttatagtgca attaaagact tacaacgcaa
      601 agcacctgat aaaaatatcg ttatttttac ccataatcat tgcctgacat atattgctaa
      661 agataagcgt gatgcgacat ttaaacctga ttatctggat ggtttagtca tgcatgtgga
      721 aaaaggcaaa gtttatctgg atggggaatt cgttaaccac taaattttca atctgacagc
      781 cagtgatggc tgtcatcatt gttaaagaag acctttcaaa cgtaacgttt ttcgggtggc
      841 aacattcaaa tcataatgca ccagatcttc aggttttacc cacgcgtagt cctgaaactc
      901 ttcgtttatt ttcacatctc ggttggcaga aacgcagtca aaaatcaggt aaatcatata
      961 aatctcttcc ttgcgaccat ctgcatacgt cttggtgcga atatcatcgc tgaaggtcca
     1021 cggcgtgatt tctgtcaaaa gcagctgttc tcccagctct tcgcgaattt cgcggcgtag
     1081 tgcctcttca attcgttcgc caggctccac gccgccaccc gaaagcgccc attgaccggg
     1141 gaaaacgccg cgatcgtcgg ccattttaca cagcaaataa gcaccatcat tttgaatcaa
     1201 agggcataca atagtccgtt gtc
//
LOCUS       DAZFJL010000302         1191 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZFJL010000302 DAZFJL010000000
VERSION     DAZFJL010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.305"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            38..1120
                     /locus_tag="ACHJ8C_004763"
     CDS             38..1120
                     /locus_tag="ACHJ8C_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFO5554449.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKLGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
BASE COUNT          340 a          252 c          270 g          329 t
ORIGIN      
        1 tcatttttaa ataatagttc aaataaaagg gctcacgatg aaaaaattaa cagtggcaat
       61 ttctgctgta gctgcatcag tactgatggc gatgtctgct caggcagcag agatttacaa
      121 taaagacagt aacaagctgg atctgtacgg aaaagttaat gctaagcact acttctcttc
      181 taacgatgca gatgatggcg acacaactta tgttcgtctg ggcttcaaag gcgaaactca
      241 aatcaacgat caactgactg gtttcggtca gtgggaatat gaattcaaag gcaaccgtgc
      301 tgaatctcaa ggttcttcca aagataaaac tcgtcttgcc ttcgctggcc tgaaattggg
      361 tgactacggc tccatcgatt atggccgtaa ctacggtgta gcatacgata tcggtgcatg
      421 gaccgacgtt ctgccagaat tcggtggcga tacctggacc caaacagatg tgttcatgac
      481 tggtcgcacc acaggtgttg caacctatcg taacaacgac ttctttggtc tggttgatgg
      541 tctgaacttt gctgctcagt atcagggtaa aaatgaccgc actgacgtaa ctgaagctaa
      601 cggtgatggt ttcggtttct ccactactta tgagtatgaa ggattcggtg taggtgcaac
      661 ctatgctaaa tctgaccgca ctaatgatca ggttatctac ggtaacaaca gcctgaatgc
      721 atctggtcaa aatgctgaag tatgggcagc tggtctgaaa tatgatgcga acaacatcta
      781 tctggctacc acctattctg aaacccagaa catgactgtt tttggtaata accatattgc
      841 caacaaagca caaaacttcg aagtagttgc acaatatcag ttcgacttcg gtctgcgtcc
      901 gtccgttgct tacctgcaat ctaaaggaaa agacttgggt gcgtggggtg atcaggacct
      961 ggttgaatat attgatgtag gtgcaaccta ttacttcaac aaaaatatgt ccacttttgt
     1021 tgattacaaa atcaacctga ttgataagag cgatttcacg aaagcatctg gcgttgctac
     1081 cgatgatatc gttgctgtag gattggttta ccagttctaa tctgattact caaagatatg
     1141 ttgtgggagg ctttgcctcc ccaacatata agtggcgccc tcaagccact t
//
LOCUS       DAZFJL010000303         1190 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFJL010000303 DAZFJL010000000
VERSION     DAZFJL010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.306"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..358
                     /locus_tag="ACHJ8C_004764"
     CDS             <1..358
                     /locus_tag="ACHJ8C_004764"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554450.1"
                     /translation="VFIQFIYSFVLIIFRRLLPFLRNKFPSVFSYTGTLTYLFMSISL
                     AVSFIVAANAVIDTVSSSAVMNRYLIVAFSYLPHNFTFCANLVLAADFISFLFHSKDR
                     IYRYIRLFISGKVSGV"
     gene            362..>1190
                     /locus_tag="ACHJ8C_004765"
     CDS             362..>1190
                     /locus_tag="ACHJ8C_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HFO5554451.1"
                     /translation="MPVITGNLGQGKGIVAAYFASLYYRRGLRVAANYPLNTECMCSG
                     SDNPVTVIPAMPRIEDLELLGRGCPENEKTRFGALFLDECATWLNTRGFARKDRLPLI
                     DWLIHSRKLGWDVYLIAQHEDMIDSQIIKAMGAKIIRCRRLDELRVPVITPLMELFRP
                     GKTGIASGKRGIIPHYVTASSFLYDGTTHAARRPVDKVVIKAADYYNVYDTNFIFSDG
                     MELLNGRFVDMRAVYSVLPGRMLMTMNQLPEQSDKKAAPQKKPWGKLIAFLFLVVVMI
                     "
BASE COUNT          268 a          222 c          271 g          429 t
ORIGIN      
        1 tgtttttata cagtttattt attcttttgt tcttattatt tttaggagat tacttccttt
       61 tctcaggaat aaattccctt ctgttttttc ttatacaggt acgttaacgt atctttttat
      121 gtcgatttct ttggctgtaa gttttatcgt tgcagccaat gctgttattg atacggtttc
      181 ttcttctgcg gttatgaaca gatatcttat tgttgcgttc agttatttac ctcataactt
      241 tacattttgt gcgaatcttg ttcttgcggc tgattttatt tcttttcttt tccattcaaa
      301 ggatcgtatt tatcgttaca ttcgattatt tatatcagga aaagtttcag gagtataatt
      361 tatgcccgtt attacaggga atctgggtca gggtaaaggc attgtggcgg cttattttgc
      421 ctcgctttat tatcgccgtg ggttgcgggt tgctgcaaat tacccgctta acactgagtg
      481 catgtgttcc ggttcggata atccggttac ggttattcct gccatgccgc gaattgaaga
      541 cctggaactt ctcgggcgtg ggtgtcctga aaatgaaaaa acacgcttcg gggcgttatt
      601 tcttgatgag tgtgcgacat ggctgaatac ccgtggtttt gcccgtaagg acagattacc
      661 gttaatcgac tggctgatac attcccgtaa gctgggctgg gatgtttatc tgattgcaca
      721 gcatgaagat atgattgatt cccagataat taaggcgatg ggggctaaaa ttattcgctg
      781 ccgccgtctt gatgaattac gtgttcctgt gattacgccg ctgatggaat tattcagacc
      841 gggaaaaacg gggattgcat cggggaaaag ggggattatt cctcattatg ttaccgccag
      901 ttcgtttctt tatgatggca ccactcatgc tgcccgacgt cctgttgata aggttgttat
      961 taaggccgct gattattaca acgtttatga caccaatttt attttttctg acgggatgga
     1021 actgcttaat ggtcgttttg tggatatgcg cgccgtttat tccgttttgc caggacgaat
     1081 gcttatgacc atgaatcagt tacctgaaca atccgataaa aaagctgctc cacagaaaaa
     1141 accatgggga aagttgattg cctttctttt tcttgtcgtg gtgatgattg
//
LOCUS       DAZFJL010000304         1180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFJL010000304 DAZFJL010000000
VERSION     DAZFJL010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.307"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..150
                     /locus_tag="ACHJ8C_004766"
     CDS             <1..150
                     /locus_tag="ACHJ8C_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5554452.1"
                     /translation="DENGIIPEQDRVITQVVILDTDKKLIQCVVRPLQILRADGTWEN
                     IGGMK"
     gene            327..>1180
                     /gene="nleB2"
                     /locus_tag="ACHJ8C_004767"
     CDS             327..>1180
                     /gene="nleB2"
                     /locus_tag="ACHJ8C_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB2"
                     /protein_id="HFO5554453.1"
                     /translation="MLSPIRTTFHNSVNIVQSSPCQTVSFAGKEYELKVIDEKTPILF
                     QWFEPNPERYKKDEVPIVNTKQHPYLDNVTNAARIESDRMIGIFVDGDFSVNQKTAFS
                     KLERDFENVMIIYREDVDFSMYDRKLSDIYHDIICEQRLRTEDKRDEYLLNLLEKELR
                     EISKAQDSLISMYAKKRNHAWFDFFRNLALLKAGEIFRCTYNTKNHGISFGEGCIYLD
                     MDMILTGKLGTIYAPDGISMHVDRRNDSVNIENSAIIVNRSNHPALLEGLSFMHSKVD
                     AHPYYDGLG"
BASE COUNT          389 a          165 c          277 g          349 t
ORIGIN      
        1 gatgagaatg gcattattcc ggagcaggac agggtaatca cacaggtggt gatactggat
       61 acagataaaa agctgataca gtgtgtggta agaccgctgc aaatcctgcg tgctgacggg
      121 acgtgggaaa atattggcgg gatgaagtaa cccgacagct tcacaaaacc ggagtccggc
      181 tccggttttt gttgtcatgt catggtgatg tttgttagga aagcaaagat ggcaaaactg
      241 ctggaggttt tgtggttgag tatgccaata taattaatag attaaagagt tagttgtgaa
      301 gaaaatatgg ataaacagga cgacgaatgc tttcaccgat aaggacaact ttccataact
      361 cagtaaatat agtgcagagt tcaccctgtc aaacggtttc ttttgcagga aaggaatatg
      421 agttaaaggt cattgatgaa aaaacgccta ttctttttca gtggtttgaa cctaatcctg
      481 aacgatataa gaaagatgag gttccaatag ttaatactaa gcagcatccc tatttagata
      541 atgtcacaaa tgcggcaagg atagagagtg atcgtatgat aggtattttt gttgatggcg
      601 atttttcagt caaccaaaag actgcttttt caaaattgga acgagatttt gaaaatgtaa
      661 tgataatcta tcgggaagat gttgacttca gtatgtatga cagaaaacta tcagatattt
      721 atcatgatat tatatgtgaa caaaggttac gaactgaaga caaaagagat gaatacttgt
      781 tgaatctgtt agagaaagag ctgagggaaa tttcaaaggc gcaggattct ttgatttcta
      841 tgtatgcaaa gaaaagaaat catgcatggt ttgatttctt cagaaattta gccttattaa
      901 aagcaggaga gatattcagg tgcacatata atacaaagaa tcacggtatt tcattcgggg
      961 aggggtgtat ctatcttgat atggatatga tacttacagg taagcttggt acaatatatg
     1021 ctcctgatgg aatttcaatg catgtggatc gtcgtaatga tagtgtaaat attgaaaata
     1081 gtgcaataat tgttaaccgt agtaatcatc ctgctctact tgagggactt tcttttatgc
     1141 atagtaaagt agatgctcat ccatattatg atggtttggg
//
LOCUS       DAZFJL010000305         1165 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZFJL010000305 DAZFJL010000000
VERSION     DAZFJL010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.308"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(241..819)
                     /locus_tag="ACHJ8C_004768"
     CDS             complement(241..819)
                     /locus_tag="ACHJ8C_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO5554454.1"
                     /translation="MPSLVSGIQSSSFSTGMQVLRAQMAASGGGEITVGGQTVSITYS
                     ETDGRFLASGGNNSLLSGLLLTGLNGGPEALRDIMLRMVSGSGNTQSHGDIEGKISQY
                     KFSVNTQSLQCPSEAVRCPIILDKPEEGVFVKNSEDSLVCTLFDSVSFSHLVRDGGRH
                     PLTREPITSSMIVSPEQCIYDQAKGNFVIKDK"
     gene            complement(932..>1165)
                     /locus_tag="ACHJ8C_004769"
     CDS             complement(932..>1165)
                     /locus_tag="ACHJ8C_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5554455.1"
                     /translation="GCGKHDGREHVQSLTAQLRLGPADILESDENGIIPEQDRVITQV
                     VILDADKKQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT          315 a          315 c          211 g          324 t
ORIGIN      
        1 accggacatg acaacaaaaa ccggagctgg actccggttt ttgtgaactc gtcggctatt
       61 tcatcccgcc aatatttccc cgtcagcacg actgattagc ggcctctcca ctcactgtat
      121 atgcttttta tctgcatcca gtacaccacc tgtgttatta ccacatcctt ataccctgaa
      181 catctgtagc atttcatata agcaactggc agttaacata tagcttgggt aatatttatt
      241 tcacttatcc tttatgacaa agtttccttt ggcttggtca taaatacatt gttctggact
      301 tacaatcatt gatgacgtta ttggttctcg tgttagcggg tgcctcccac cgtcacgaac
      361 caaatgagaa aaagaaaccg aatcaaataa ggtacaaacc aaagaatctt ctgaattttt
      421 aacaaacaca ccttcttctg gcttatccag tataattggg catcgaaccg cctcggatgg
      481 gcactgaagg ctctgcgtat taaccgaaaa cttatattgt gatattttcc cctcaatatc
      541 accatgtgat tgtgtgttac ctgaacctga aaccattctt aacattatat ccctgagcgc
      601 ttcaggacca ccattaagcc ctgtaagtaa taatccagaa agcaaactgt tattgccccc
      661 actcgccaga aagcgaccat ccgtttcact atatgtgata ctaaccgtct gtccgcctac
      721 tgtaatttct ccaccaccac tggcggccat ttgagcacga agaacctgca ttccggtgga
      781 aaatgaggat gattgaatac ctgaaactaa tgatggcatg acaaaactcc ctatttaaat
      841 attcattcca aacacaggga gtcataacaa acatcaccat ggcatgacaa caaaaaccgg
      901 agccggactc cggttttgtg aagctgtcgg gttacttcat cccgccaata ttttcccacg
      961 tcccgtcagc acgcagaatt tgcagcggtc ttaccacgca ctgtatctgc tttttatccg
     1021 catccagtat caccacctgc gtgattaccc tgtcctgctc cgggataata ccattctcat
     1081 ctgactccag gatgtctgcc ggccccagtc gcagttgtgc tgtaagcgac tgcacgtgtt
     1141 cacggccatc atgctttccg caacc
//
LOCUS       DAZFJL010000306         1152 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFJL010000306 DAZFJL010000000
VERSION     DAZFJL010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.309"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            25..747
                     /gene="sdiA"
                     /locus_tag="ACHJ8C_004770"
     CDS             25..747
                     /gene="sdiA"
                     /locus_tag="ACHJ8C_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO5554456.1"
                     /translation="MQDTDFFSWLRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(815..1039)
                     /gene="yecF"
                     /locus_tag="ACHJ8C_004771"
     CDS             complement(815..1039)
                     /gene="yecF"
                     /locus_tag="ACHJ8C_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO5554457.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(1026..>1152)
                     /gene="yecU"
                     /locus_tag="ACHJ8C_004772"
     CDS             complement(1026..>1152)
                     /gene="yecU"
                     /locus_tag="ACHJ8C_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO5554458.1"
                     /translation="DITLKKQPLLFLAKLMVYSAALTFFTANFHCNMTRKINEYA"
BASE COUNT          296 a          248 c          280 g          328 t
ORIGIN      
        1 ctctcagggg cgttgcggtt tactatgcag gatacggatt ttttcagctg gcttcgcacg
       61 atgttgttgc gttttcagag gatggaggcc gcagaagagg tctaccatga aatagagctt
      121 caggctcagc agctggagta cgattactat tcgttatgtg ttcgccaccc ggtaccattc
      181 acgcgaccta aagtggcttt ctataccaat taccctgagt cgtgggttag ttattatcag
      241 gcaaaaaatt ttctcgcaat tgatccggtg ctgaaccctg aaaactttag tcagggccat
      301 ttaatgtgga atgatgactt attcagcgaa gcacagccgt tatgggaagc tgcgcgcgca
      361 catggtttac gccgcggtgt cactcagtat ttaatgctgc caaatcgggc gttgggcttt
      421 ttgtcctttt cccgttgcag cacgcgcgaa atacccattc ttagtgatga actgcaatta
      481 aaaatgcagc tactggtgcg cgaaagtctg atggctctga tgcgtttaaa tgatgaaata
      541 gtgatgacgc cagagatgaa tttcagcaag cgcgaaaaag aaattctgaa atggacggcg
      601 gaaggtaaga catcagcaga gatagcgatg attttgtcaa tctctgagaa tacggtcaat
      661 ttccatcaga aaaacatgca gaaaaaaatc aatgcaccga ataagacgca ggttgcctgt
      721 tacgcggccg ctactggctt aatttgatct cttttctgtt ctgcgtacca gatgcaaaaa
      781 ccggctgaaa ggcacgctct cagccggttt tatattactg acggtaggct tgtttaattt
      841 gcttaaccgt tttggaaaat actgcagcct gggtttcgtc ttcgatcagc gcaagctgtt
      901 tttccatctt taacatcacg cggcccgcgt cagcttgtcc catcgcctgc agcatcagcg
      961 tcagcatcgc tttcaggcag gagacttcat ttgccagttc ttgattattc tcggcagtag
     1021 aaaaatcagg cgtactcatt tattttcctc gtcatgttgc aatgaaaatt tgcggtgaaa
     1081 aatgttaagg cggcggagta taccataagc tttgctaaaa atagcagtgg ttgttttttg
     1141 agcgtgatat ca
//
LOCUS       DAZFJL010000307         1132 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFJL010000307 DAZFJL010000000
VERSION     DAZFJL010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.310"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..625
                     /locus_tag="ACHJ8C_004773"
     CDS             <1..625
                     /locus_tag="ACHJ8C_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFO5554459.1"
                     /translation="DFDKRKMEFTYPEANKNHLQSNNSDVVAAAAISIAATEMKTILP
                     NDLTPGKYNKIYLSGDGSAGLPLLKCGDEFLSPADIVERIVQHNLHEMDDIRLTSCNS
                     ADIIKNKDFSPDEIEKSANINNGWLARALFGQKRSLAEHVYAEFERRGINVSISGYHG
                     TGVFYVPEHGKPTTHLRSTTVPATPEHTVRRSDYRATLGRTQPIDID"
     gene            712..864
                     /locus_tag="ACHJ8C_004774"
                     /pseudo
     CDS             712..864
                     /locus_tag="ACHJ8C_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          358 a          210 c          233 g          331 t
ORIGIN      
        1 agattttgat aaaagaaaaa tggagtttac atatcctgaa gcaaataaaa accatctaca
       61 atcaaataat agcgatgttg ttgccgctgc agctataagt attgcagcga cagaaatgaa
      121 aaccattctg ccaaatgatt taaccccagg aaaatacaac aaaatttatc tgtcagggga
      181 tggttctgct ggcctacccc ttcttaagtg cggagatgaa tttttatcgc cggcagatat
      241 tgtcgaacgc attgttcaac ataatcttca tgaaatggat gatatcagat taacatcctg
      301 taactcagcc gacataataa aaaacaaaga cttctctcct gatgaaatag agaaatccgc
      361 aaatatcaat aatggctggt tggctagggc attatttggt caaaagaggt ctttagcaga
      421 acatgtctat gccgagtttg aacgtcgcgg aattaacgtt tctatatcag gttaccatgg
      481 cacaggcgtt ttttatgtac cagagcatgg taaaccaaca acgcatctac gctccacaac
      541 tgtgcctgca acacctgaac atactgtaag aagaagtgac tacagagcca ctttgggtag
      601 aactcaaccc atagatattg actaattgag tcagcatata cacttgatgg ttaggaaact
      661 gaatcattgt tttaaacatt tcctagccat taatattgtc gtcgggagcg caattatggc
      721 tggaggttgt ggcaagtgct tcatgagaca gtaacccgaa atcaccagtg ccattcaatg
      781 tggcaactgt tgaaaaaggt tcgtcacttt atggataccg tcagctcatt ccccggtaga
      841 aaacatgggc tggctaaagt gtagcggtat taggatcagt tatttagtag ttgtgttatt
      901 ttttatattt cattcttatt gattctgttt agaaatagcc ataatggatg atgatgtggc
      961 gtggcaaagc agagttacac aaagtctgcc acatgttttt atgtcaaaaa attagcgcaa
     1021 gaagacaaaa aatcaccttg cgctaatgct ctgttacagg tcactaatac catctaagtc
     1081 gttgattcat agtgactgca tatgttgtgt tttgtagtat tatgtagtct gt
//
LOCUS       DAZFJL010000308         1107 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFJL010000308 DAZFJL010000000
VERSION     DAZFJL010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.311"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            74..1051
                     /locus_tag="ACHJ8C_004775"
     CDS             74..1051
                     /locus_tag="ACHJ8C_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO5554460.1"
                     /translation="MSDSVTVGIDVSKLTLDVHVSPAQLRQTLSNSDAGFEELKTLLS
                     MQQVSLVLLESTGGYEDAVVCFLQSLGYDVSVINPRQARDFARSMGRLAKTDRIDAEL
                     LAQLASVIDSRPDRARFVKKITDDRRRKLACMVTRRRQVVNMISTEQQRKSAASVYST
                     ESINRVLSFLRSELKSIDADISLHVRSHFADISGLLASFKGIGPATIGVLLGEVPELG
                     RLNRRQISALIGVAPFNRDSGIMRGRRTVWGGRTMVRNALYMPTLSAVRFNPVIREFY
                     TRLISQGKPRKLAQVAAMRKLLCIINAMLSSGTMFNPALHLHSVQEAQQ"
BASE COUNT          233 a          282 c          270 g          322 t
ORIGIN      
        1 tctgctctta tccaagcccg tattgttgac tgaaccaggt gcaaagttcg cacaccagga
       61 agggataaat aagatgtcag acagtgtaac agttggtatc gatgtatcaa aacttactct
      121 tgatgttcat gtgtctcctg cacagcttcg tcagactcta tcaaactccg acgctggctt
      181 tgaggagctg aaaacattgc tttccatgca gcaggtttct cttgttcttc tcgaatccac
      241 ggggggttat gaggatgccg ttgtctgctt cctgcagtct cttggttatg acgtttctgt
      301 cattaatcct cgtcaggctc gtgattttgc ccgctctatg ggacgtctgg ctaaaaccga
      361 caggattgat gctgagctgc ttgcccagct tgcttctgtt attgattccc gccctgaccg
      421 tgcgcgtttt gtcaaaaaaa ttaccgatga caggcgtcgt aagctggcct gcatggtcac
      481 ccgtcgccgt caggttgtga atatgatttc cactgaacag cagcgtaaat ctgccgccag
      541 tgtgtattcc actgaaagta ttaatagggt tttatctttt ctgcgctctg agctgaaaag
      601 tattgatgct gatatttcac tgcatgtccg ctctcatttt gcagatattt ccggtcttct
      661 tgcctcattt aaggggattg gtcctgccac aattggcgta ctgctgggtg aggttcctga
      721 actgggccga ctgaaccgcc gtcagattag cgcactgatt ggcgtggctc cgtttaaccg
      781 tgactccggc attatgcgcg gtcgccgtac ggtatggggc ggtcgtacaa tggtccggaa
      841 cgcgctttac atgccaacgc tttctgccgt acgttttaac ccggttatcc gggagtttta
      901 tacgcggctg atttctcagg gtaagcccag aaaactggct caggttgcgg ccatgcgtaa
      961 attgctctgc atcattaatg ccatgctttc ttcagggaca atgtttaacc ctgccctgca
     1021 cctccattca gttcaggagg ctcaacaatg aaaacggaat cagccatcct cacagcctat
     1081 aacgccatcg acgcggcatc cgttgct
//
LOCUS       DAZFJL010000309         1079 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFJL010000309 DAZFJL010000000
VERSION     DAZFJL010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.312"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            69..>1079
                     /gene="waaB"
                     /locus_tag="ACHJ8C_004776"
                     /gene_synonym="rfaB"
     CDS             69..>1079
                     /gene="waaB"
                     /locus_tag="ACHJ8C_004776"
                     /gene_synonym="rfaB"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,6-galactosyltransferase"
                     /protein_id="HFO5554461.1"
                     /translation="MKIAFIGEAVSGFGGMETVIRDVITTFRQQHIYSEMFFFCRNDK
                     MDKGWLEGIKYSCSFSNIRLGFLRRAKHIHALSKWLQDYQPDVVICIDVISCLYAAKA
                     RKKSGIDVPVFSWPHFSLDHKKHAGYITCADYHLAISSGIKQQMINRGVPESTINVIF
                     NPVEAKDTVIPGPEEGETATFIYVGRMKFEGQKRVKDLLDGLSQAQGDWKLHVLGDGS
                     DFEKCQAYGRELKIDNRIVWHGWQKHPWEVVRQDIKKVSALLLTSSFEGFPMTLLESM
                     SWGIPCISADCVSGPGDIIQPDVNGHLYQPGDIAGFVTLLNKYIAGEIHIEHEKIPAS
                     IDK"
BASE COUNT          317 a          175 c          245 g          342 t
ORIGIN      
        1 tgtttatgat tttatagaaa aataaaaccg ttaaaacagt gagttttact caattaatga
       61 gagtttcaat gaaaatagca tttatcggcg aagctgtttc tggatttggc ggcatggaaa
      121 cggtcattcg tgatgttatt actaccttca ggcaacaaca tatctatagt gaaatgtttt
      181 tcttttgtcg taatgataaa atggataagg gatggctgga aggtattaag tactcgtgtt
      241 cattttccaa tattcgctta ggttttttgc gtcgggcgaa acatattcat gctttaagca
      301 aatggcttca agattatcag ccagatgttg ttatttgtat tgacgttatc tcgtgtttgt
      361 atgcggccaa agcacgtaaa aaatcaggaa ttgatgtgcc agtattttca tggccgcatt
      421 tttctttaga tcacaaaaaa catgctggat atatcacctg tgcagactat catttagcca
      481 tcagctctgg cattaaacag caaatgatta atcggggtgt accagaatcg acgataaatg
      541 ttatttttaa tccagtcgaa gctaaagaca cagtcattcc tgggcctgag gaaggtgaaa
      601 cggcaacgtt catttatgtt gggcgtatga aattcgaggg ccagaagagg gttaaagacc
      661 tgcttgatgg tttatcacaa gcgcaaggtg actggaaact ccatgtactg ggtgatggtt
      721 ctgattttga aaaatgccag gcatatggta gagaattgaa gattgataat cgaattgtct
      781 ggcatggttg gcaaaaacat ccatgggagg tagtgcgtca ggatattaag aaagtcagtg
      841 cattgttatt aacctcatct tttgaaggtt ttccaatgac cttacttgaa tcaatgtcat
      901 gggggattcc gtgtatcagt gccgattgtg tctccggtcc tggtgacatt attcaacctg
      961 atgtcaatgg ccatttgtat caaccgggag atattgctgg ttttgttact cttctaaata
     1021 aatatatagc aggtgaaatt catatcgaac atgaaaaaat tcctgcgtcg atcgataag
//
LOCUS       DAZFJL010000310         1078 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFJL010000310 DAZFJL010000000
VERSION     DAZFJL010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.313"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>1078
                     /locus_tag="ACHJ8C_004777"
     CDS             join(<1..172,172..>1078)
                     /locus_tag="ACHJ8C_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO5554462.1"
                     /translation="DAAKAMDIGLSTMTRWVKQLRDERQGKTPKASPITPEQIEIREL
                     RKKLQRIEMENEIFKKGYRALDVRLPEQFSIIGKLRAHYPVVTLCHVFGVHRSSYRYW
                     KNRPEKPDGRRAVLRSQVLELHNISHGSAGARSIATMATLIGFRMGRWLAGRLMKELG
                     LVSCQQPAHRYKRGGREHVTIPNHLGRQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVL
                     DLFARKPVGWAMSFSPDSRLTIKALKMAWEIRSKPAGVMFHSDQGSHYTSRQFRQLLW
                     RYQIKQSLSRRGNCWDNSPMERFFRSLKNEWIPVTGYMNFSDAAHEITDYIVGYYNAL
                     RPHEYNGGLPPNESENRYWKNSKAVA"
BASE COUNT          308 a          260 c          295 g          215 t
ORIGIN      
        1 gatgccgcca aagctatgga tatcggcctt tccacaatga caagatgggt caaacaactg
       61 cgtgatgagc gtcagggcaa aacaccaaaa gcctctccga taacaccaga acaaatcgaa
      121 atacgtgagc tgaggaaaaa gctacaacgc attgaaatgg agaatgaaat attaaaaaag
      181 gctaccgcgc tcttgatgtc agactccctg aacagttctc gataatcggg aaactcagag
      241 cgcattatcc tgtggtcaca ctctgccatg tgttcggggt tcatcgcagc agctacagat
      301 actggaaaaa ccgtcctgaa aaaccagacg gcagacgggc tgtattacgc agtcaggtac
      361 ttgagttgca taacatcagc catggttctg ccggggcaag aagcatcgcc acaatggcaa
      421 ccctgatagg cttcagaatg gggcgctggc ttgccggcag gctcatgaaa gaactgggac
      481 tggtcagttg ccagcagcct gcgcaccgtt ataaacgagg tggtcgtgaa catgtcacta
      541 tcccgaatca ccttgggcgg cagttcgcag tgacagagcc aaatcaggta tggtgcggcg
      601 acgtgacgta catctggacg gggaaacgtt gggcatacct tgccgttgtt ctcgacctgt
      661 ttgcaaggaa accggtaggt tgggcaatgt cgttctctcc ggacagcaga ctgaccatca
      721 aagcgctgaa aatggcctgg gaaatccgca gtaaaccagc cggggtaatg ttccacagcg
      781 atcagggcag ccactataca agcaggcagt tccggcagtt actgtggcgt taccagatca
      841 aacagagtct gagtcgacga ggaaattgct gggataacag cccgatggag cgcttcttca
      901 ggagtctgaa aaacgagtgg ataccggtga cgggttacat gaacttcagc gatgctgccc
      961 atgaaataac ggactatatc gttgggtatt acaacgcgct caggccgcac gaatataacg
     1021 gtgggttgcc accaaatgaa tcggaaaacc gatactggaa aaactctaaa gcggtggc
//
LOCUS       DAZFJL010000311         1073 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFJL010000311 DAZFJL010000000
VERSION     DAZFJL010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.314"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..162)
                     /locus_tag="ACHJ8C_004778"
     CDS             complement(<1..162)
                     /locus_tag="ACHJ8C_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir chaperone family protein"
                     /protein_id="HFO5554463.1"
                     /translation="MNEQFCKDLAHTFGITLEEQTDALAFHDNDGHEWILECAPQSEI
                     LFFYCYLSTN"
     gene            438..1049
                     /locus_tag="ACHJ8C_004779"
     CDS             438..1049
                     /locus_tag="ACHJ8C_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein Map"
                     /protein_id="HFO5554464.1"
                     /translation="MFSPTAMAGRALAQAVTQTLRPAVTKAATQAGMGVSGMRFTPMQ
                     SNLMINYGKLTNQLLQAVAKQTRSGDTQQWFQQEQTTYISRTVNRALDDYCRSNNSVI
                     SQATKGHIFRVVENALQQPLDINGAQSSIGHFLQSNKYFNQIVDEQCGKGGDPITRFN
                     TKTKMIEQASQEIFERNFNGLKVSDIKEITQNAILEHVQDTRL"
BASE COUNT          358 a          181 c          223 g          311 t
ORIGIN      
        1 gttggttgat aggtaacaat aaaaaaacaa aatttctgat tgaggtgcgc actcaagtat
       61 ccactcatga ccatcgttat cgtgaaaagc gagggcgtca gtttgctctt ccaatgttat
      121 cccaaacgta tgtgcaagat ctttgcaaaa ttgttcattc atattgtagt cctgtcattc
      181 ttataacgct gatgtgttca taggcaattt atttgtgagt tcttacacaa ataacacgga
      241 aaaaatataa catgtaagta ataatgttgc atattcatga tagcatatga taaatgtagt
      301 gcaaccacct ataaatagtg ctggaggaaa agttctggag gaaaattaaa atcataaaat
      361 aatgttatag ttttatttat tcgtttttat ttttataggt gtaaatgttt tttatgtaat
      421 ttataaaggt ttatagcatg tttagtccaa cagcaatggc aggtagagcg ttagctcagg
      481 cggttacaca aactcttaga cccgctgtaa ccaaggctgc gacgcaagcg ggcatgggag
      541 tcagtggtat gaggttcaca cctatgcagt cgaaccttat gattaattat ggcaaactga
      601 ctaaccaact tctgcaggct gtagccaaac aaactcgtag tggcgatacc caacaatggt
      661 tccagcagga gcagacgact tatatatcca gaacagtaaa cagagctctg gatgactatt
      721 gcaggagtaa taattcagtc attagtcagg cgactaaagg tcacattttt agagttgttg
      781 aaaatgcgtt acagcaacct cttgatataa acggggcgca atcatcaatt ggtcacttct
      841 tgcagtctaa taaatacttt aatcagatag ttgatgagca gtgtggcaaa gggggagatc
      901 caataacacg ttttaacacc aagactaaga tgatagaaca agcctcacaa gaaatatttg
      961 aacggaactt caatggctta aaagtcagtg atataaaaga gattactcag aatgcgattt
     1021 tagagcatgt acaggatacc cgattgtagg gataaccttg tcaggtataa att
//
LOCUS       DAZFJL010000312         1057 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZFJL010000312 DAZFJL010000000
VERSION     DAZFJL010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.315"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..758)
                     /gene="nleH"
                     /locus_tag="ACHJ8C_004780"
     CDS             complement(<1..758)
                     /gene="nleH"
                     /locus_tag="ACHJ8C_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase NleH"
                     /protein_id="HFO5554465.1"
                     /translation="MLSPYSVNLGCSWNSLTRNLTSPENRVLSSVRDAAVHSDNGAQV
                     KVGNRTYRVVATDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNASPVSAAT
                     ERGIVHSNRPDLPPVDYAQPELPSVDYNRLPVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTSEVRCFNQYYGAGSAEKIYSDNGDILGIRMNKINGESLFNIPSLPAQ
                     AEQAIYDMFDRLEQKGILFVDTTETNVLYDRAKN"
     gene            complement(928..1057)
                     /locus_tag="ACHJ8C_004781"
                     /pseudo
     CDS             complement(928..1057)
                     /locus_tag="ACHJ8C_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          311 a          265 c          172 g          309 t
ORIGIN      
        1 tcattttttg cgcgatcata taagacattg gtttctgttg tatcaacaaa aagaattcct
       61 ttttgctcca gtctatcaaa catatcgtaa atagcctgct cagcttgtgc tggtaatgat
      121 ggaatattaa aaagagattc cccatttatt ttattcattc taataccaag aatatcgcca
      181 ttatcgctat atattttttc tgcactcccg gcaccataat attggttgaa gcaacgaact
      241 tcgcttgtca cctcttcatt gctttgagat gtagtaaaca tcttcaggac ttttgttgca
      301 tcctcagcat cttcatatac tacagcgtta ccccctttgc caataacatt accaggcact
      361 ggcaacctgt tatagtccac actcggtagc tctggctgtg cataatcaac aggaggtaaa
      421 tcaggtctgt tcgaatgaac aatgcctctt tccgtagcgg cgctcactgg tgatgcattc
      481 agcatgacct caattttcct gctaatctcc cccttaggcc atcccagcct gtgcaacaga
      541 ttagtaaaac aaccactatg actttctctt gtaacgcaaa acttattatc ggtggcaaca
      601 acacgatatg ttctgttacc aacctttact tgcgccccat tatcagaatg aacggcagca
      661 tcccttacag aggataaaac acgattttca ggcgaagtca ggtttctggt taaagaattc
      721 catgaacatc ccaaatttac agaatatggt gataacatac atttcaacct tcaaaataaa
      781 cccatctaaa tttccctaac aaacatcccc ctaacatggc aacaaaaaac cagaaatgaa
      841 ctcttgcttt ttgtatctgg caactaattc atccttccag tatcccatca aacgaacgtt
      901 tacgaactat aacgcaggta ttaccggtca ctcgatatct ggagatacag acgtatcaac
      961 atgattgagc ggcacacgat actgcttcca tgccgccagt aactatgcca gctcatccgt
     1021 cgcaatatct aaatctgcag catcctgaag tggtgcg
//
LOCUS       DAZFJL010000313         1053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFJL010000313 DAZFJL010000000
VERSION     DAZFJL010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.316"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            59..901
                     /locus_tag="ACHJ8C_004782"
                     /pseudo
     CDS             59..901
                     /locus_tag="ACHJ8C_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
     gene            complement(925..1053)
                     /locus_tag="ACHJ8C_004783"
                     /pseudo
     CDS             complement(925..1053)
                     /locus_tag="ACHJ8C_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          233 a          289 c          322 g          209 t
ORIGIN      
        1 gaactgtagt acttaccaat aaataagtct cgatacggcg ctccgttaac gtttaccact
       61 accctgtgac gaaaagcgac tgttgcctgc ggcgccgtgc tgcccgaact gcggcggttc
      121 actgagctat ctgggcgagg ataccgccga acagctggag ttgatgcgta gcgccttccg
      181 ggttatccgg acggtacggg aaaaacatgc ctgtactcag tgcgatgcca tcgtgcaggc
      241 acctgcacct tcgcggccca tcgagcgggg tatcgccgga ccggggctgc tggcccgcgt
      301 gctgacctcg aagtatgcag agcacacccc gctgtatcgc cagtcagaaa tatacggccg
      361 gcaaggtgtg gagctgagcc gttcactgct gtcgggctgg gtggatgcat gctgccggct
      421 gctgtctccg ctggaagagg cgcttcatgg ctatgtcatg actaacggca aactccatgc
      481 cgatgatacc ccggtccagg tactgctgcc gggtaataag aagacgaaga ccgggcggtt
      541 gtgggcgtat gttcgtgatg accgcaatgc cgggtcagcg ttggcacctg cagtgtggtt
      601 cgcttacagc ccggacagaa aaggcatcca tccgcagact catcttgctt gcttcagcgg
      661 tgtgctgcaa gcggatgcat acgccgggtt caacgagctg tatcgcaatg gtgggataac
      721 ggaagctgcc tgctgggctc atgcccgccg aaagatccac gatgtgcacg tccgcatccc
      781 gtcagcactg acggaagaag ccctagagca gatcagtcag ttgtacgcca tagaggcgga
      841 tataagggga atgctggcag agcagcggct tgctgaacgt cagcgaaaaa cgaaaccgct
      901 gtacgcttac ctttcagtaa gtgcgacagg tactgtggag gcgctcccgt aaagacgggc
      961 gtcaagttcc cgctccgaca gacctgcggg cagaggccat gaaaggccag ctttgcagaa
     1021 gcgcacaaac atactacaaa ctgttgattt tgg
//
LOCUS       DAZFJL010000314         1027 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFJL010000314 DAZFJL010000000
VERSION     DAZFJL010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.317"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..382
                     /locus_tag="ACHJ8C_004784"
     CDS             <1..382
                     /locus_tag="ACHJ8C_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554466.1"
                     /translation="IARHYLSGGQEVHVAASPVVQDSVPPVPHLPVSTVAPEPEQKKP
                     DELPVSKEWRLAGYVRGVNPYFVLLGPSGQVRRYFSFQSWNGGATELNVEGERVTFWS
                     GPSGTASKGDVLTDKMLSFDVKSK"
     gene            502..>1027
                     /locus_tag="ACHJ8C_004785"
     CDS             502..>1027
                     /locus_tag="ACHJ8C_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system"
                     /protein_id="HFO5554467.1"
                     /translation="MTNLLTALIFLTSACVHASSPAFIVDRLPLNAAIQLAQEDVFGR
                     QYVLPPELASDTRPVTLDLSLAGDQKTQREEYVRWLRQMNISVETRNGVDHYRSFKPV
                     AAPEKMVSWVYTPLHRSPSYLATVLSGTTGRSSQASSESTSGTSSGALSGSVSSSSGS
                     FLSGEGDSLVFRGTR"
BASE COUNT          232 a          246 c          300 g          249 t
ORIGIN      
        1 cattgcccga cattatttat ccggtggtca ggaggttcat gtggctgcgt ctcctgtggt
       61 gcaggacagc gtgcctcccg tgcctcattt gcctgtctca acggttgccc ctgaacctga
      121 gcagaaaaag ccggatgaac ttccggtatc aaaggagtgg cgtcttgccg gatatgtcag
      181 gggagtaaac ccgtattttg tgttactggg gccgtcgggt caggtacggc gttatttctc
      241 attccagtca tggaacggcg gcgcgactga actgaatgtt gaaggtgaac gggtgacgtt
      301 ctggtcaggc ccgtcgggaa cggcatcaaa gggtgatgta ctgactgaca aaatgctttc
      361 gtttgatgtg aagagtaaat aagagagagc cgccaccgct cgccgcagac ccggcgaccg
      421 aacgtagtga gcgagtgggc gaggaagcgg aagagaacgg aacagacata aggcacttac
      481 gcacggtata acatgatgag aatgactaac ttactgacag cacttatttt tctgacttcg
      541 gcatgtgttc atgcctcgtc tcctgcattt attgttgacc gccttccact gaacgcggca
      601 atacagcttg cacaggagga tgtgtttgga cggcagtatg tgctgccacc ggaacttgcc
      661 agtgatacac gcccggtcac actggattta agtcttgcag gtgaccagaa gacccagcgt
      721 gaggagtatg tacgctggct ccggcagatg aatatttcgg tggaaacacg aaacggcgtt
      781 gaccattacc gctcgtttaa acccgtggct gcgccggaaa aaatggtgtc atgggtatat
      841 acgccgctac atcgatcgcc gtcctatctt gccacggtac tttcgggaac aacagggcgc
      901 tcatcacagg catcttctga gagtacgtcc ggtacgtctt cgggggcgtt atccggcagt
      961 gtatcgtcat cgtcaggttc atttttatcg ggtgagggtg attcactggt tttcagggga
     1021 acacgtt
//
LOCUS       DAZFJL010000315         1027 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFJL010000315 DAZFJL010000000
VERSION     DAZFJL010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.318"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            45..>1027
                     /locus_tag="ACHJ8C_004786"
     CDS             45..>1027
                     /locus_tag="ACHJ8C_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO5554468.1"
                     /translation="MAKVADGIRYAERVIAGEIVAGEFVRLACQRFLDDLKYGEERGI
                     YFSEPRAQHILNFYKFVPHVKGALAGQPIELMDWHVFILINIFGFVIPLVNEETGEVV
                     MRSDGSGRPVMVRRFRTAYNEVARKNAKSTLSSGIGLYMTGADGEGGAEVYSAATTRD
                     QARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCA
                     IIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYN
                     SDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLA"
BASE COUNT          250 a          231 c          301 g          245 t
ORIGIN      
        1 gatgatgttg atccgctggc ggagctttta aaagcgagag actgatggca aaagtggctg
       61 acgggatccg ctacgccgaa cgtgttattg caggagaaat tgtcgctggc gaatttgtcc
      121 gtctggcctg ccagcgtttt cttgatgatc tgaagtacgg cgaagagcgg gggatttatt
      181 tcagtgaacc ccgtgcgcag cacatcctga atttctacaa atttgtgcct catgtaaaag
      241 gggcgctggc aggccagcct attgagttga tggactggca tgtatttatc ctcattaata
      301 tttttggttt tgtcattccg ctggtgaatg aagagaccgg ggaagttgtc atgcgcagcg
      361 atggcagcgg gcgcccggtg atggtgcgcc ggttccggac agcatacaac gaagttgccc
      421 gtaaaaacgc aaaatcaacc ctgtcatcgg gtatcggtct gtatatgacg ggggcagatg
      481 gtgaaggcgg ggctgaggtg tattcagccg caaccacgcg tgaccaggcc agaatcgtgt
      541 ttgaagacgc caaaaatatg gtcagaaaag cccggtcgac actcgggcgg ttgtttgatt
      601 tcaacaagct ggcgatttac caggagcaga gcgcatcaaa atttgaaccg ctttcttcgg
      661 atgcaaacaa cctggatggt ctgaacatcc actgcgccat tattgatgag ctgcatgcac
      721 ataaaacccg cgacgtgtgg gacgttctgg aaacggcaac tggtgcccgt ctgcagtccc
      781 ttttatttgg tatcaccacg gctggcttta acaaggaagg gatttgttac gagcaacgcg
      841 attacgccat caaggtattg cgaggctata acagcgacgt ggagggcgcg gtaaaagacg
      901 actcctactt tgcgattatt tacaccctcg atgagggaga tgatccgttt gatgaaacgg
      961 tctggcagaa agcgaatccc ggcctgggca tctgtaaacg ctgggatgat ctgcgtcgtc
     1021 tggcaaa
//
LOCUS       DAZFJL010000316         1026 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFJL010000316 DAZFJL010000000
VERSION     DAZFJL010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.319"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..416)
                     /locus_tag="ACHJ8C_004787"
     CDS             complement(<1..416)
                     /locus_tag="ACHJ8C_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO5554469.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDPKKTAKKDEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDAEGYTYRTETQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRA"
     gene            complement(565..915)
                     /locus_tag="ACHJ8C_004788"
     CDS             complement(565..915)
                     /locus_tag="ACHJ8C_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO5554470.1"
                     /translation="MPSLIPRACRKRGCAGSTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWDAIRARILKRDNHLCQNCLRNGRAVEARTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIN"
     gene            complement(958..>1026)
                     /locus_tag="ACHJ8C_004789"
     CDS             complement(958..>1026)
                     /locus_tag="ACHJ8C_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO5554471.1"
                     /translation="RLMTMQMQLEGAQEYIRTQCIK"
BASE COUNT          214 a          286 c          255 g          271 t
ORIGIN      
        1 gcccggcttg ccggtgacat accaaactct gcaagcatcg cccggatccg cttccaggca
       61 tccgccttca tcgcagcagc cgggtgtgcc ttaatcagca catcaccgtt ctgcgtttcc
      121 gtgcggtagg tataaccctc agcatcgagt gtttcacagt gatgccggta ttcggtgtag
      181 gcttccacca gtaactcgag cgcacgtgca tcgagctgag aaatgatccc ttccgcattc
      241 aactcttccg ccattcgcct gaaccagtac ttcccctgcg cccctaaatg ttgcggaatt
      301 ttaggaagac ctttttcgtc ctttttagcg gtttttttgg ggtctttaac gggacgtttt
      361 gaggggttgc ctcgtatcaa atgcaggcgt ggcggggttt tcgggggtcc tgacataatc
      421 ggttttacct atcaatcgtt taatcacatt cccaaaaaaa agttttcgaa cctgcggcga
      481 tgcgaggaag ggtcaggcgg cggtactgag cagccagggc tgcagagatt tgacccgccc
      541 ctcccctaca gatgggaact gttatcaatt gatgcgttcg cgcgctgttt ttgctttatg
      601 gcagggccag cacagactct gcaggttact gtctgcatct gtgccaccat gagctttcgg
      661 aatgatgtgg tccacagttc tggcttcaac ggctctccca ttgcgcaggc agttctgaca
      721 cagatgatta tcacgcttca gtatacgcgc acgtatggca tcccatttcg agccatagcc
      781 acgctggtgg cgactcagtc cgcgttgatg ctgtacccat ccttcgccac gatgtttatc
      841 gcagtaacca gaactgtctg tggttgaacc tgcacatcca cgcttacggc aggcacgtgg
      901 aattagtgat ggcataaata cctcataccc tgcgaaatgt ttaccacgat aaaaaggcta
      961 cttaatgcac tgagtgcgga tatactcctg tgccccttcc agttgcatct gcatcgtcat
     1021 cagccg
//
LOCUS       DAZFJL010000317         1025 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFJL010000317 DAZFJL010000000
VERSION     DAZFJL010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.320"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..416)
                     /locus_tag="ACHJ8C_004790"
     CDS             complement(<1..416)
                     /locus_tag="ACHJ8C_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO5554472.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDPKKTAKKDEKGLPKIPQHL
                     GSQGKYWFRRMAEELNAEGIMSQLDARALELLVEAYTEYRHHCETLDAEGYTYRTETQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRA"
     gene            complement(564..914)
                     /locus_tag="ACHJ8C_004791"
     CDS             complement(564..914)
                     /locus_tag="ACHJ8C_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO5554473.1"
                     /translation="MPSRIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWDAIRARILKRDNHLCQNCLRNGRAVEARTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIN"
     gene            complement(957..>1025)
                     /locus_tag="ACHJ8C_004792"
     CDS             complement(957..>1025)
                     /locus_tag="ACHJ8C_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO5554474.1"
                     /translation="RLMTMQKQLEGAQEYIRTQCIK"
BASE COUNT          209 a          287 c          257 g          272 t
ORIGIN      
        1 gcccggcttg ccggtgacat accaaactct gcaagcatcg cccggatccg cttccaggca
       61 tccgccttca tcgcagcagc cgggtgtgcc ttaatcagca catcaccgtt ctgcgtttcc
      121 gtgcggtagg tataaccctc agcatcaagt gtttcgcagt gatgccggta ttcggtgtag
      181 gcttccacca gtaactcgag tgcacgcgca tcgagctgag acatgatccc ttccgcattc
      241 agttcttccg ccattcgcct gaaccagtac ttcccctgcg accctaaatg ttgcggaatt
      301 ttagggagac ctttttcatc ctttttagcg gtttttttgg ggtctttaac ggggcgcttt
      361 gaggggttgc ctcgtatcaa atgcaggcgt ggcggggttt tcgggggccc tgacataatc
      421 ggttttacct atcaatcatt taatcacatt ccaaaaaaaa gttttcgaac ctgcggcgat
      481 gcgaggaaag gtcaggcggc ggtactgagc agccagggtt gcagagattt gacccgcccc
      541 tcccctacag atgggaactg ttatcaattg atgcgttcgc gcgctgtttt tgctttatga
      601 cagggccagc acagactctg caggttactg tctgcatccg tgccaccatg agctttcgga
      661 atgatgtggt ccacagttct ggcttcaacg gctctcccat tgcgcaggca gttctgacac
      721 agatgattat cacgcttcag tatgcgcgca cgtatggcat cccatttcga gccatagcca
      781 cgctggtggc ggctcagtcc gcgttgatgc tgtacccatc cttcgccacg atgtttatcg
      841 cagtaaccag aactgtctgt ggttgtacct gcacatccac gcttacggca ggcgcgtggg
      901 attcgtgatg gcataaatac ctcataccct gcgaaatgtt taccacgata aaaaggctac
      961 ttaatgcact gagtgcggat atactcctgt gccccttcca gttgcttctg catcgtcatc
     1021 aaccg
//
LOCUS       DAZFJL010000318         1024 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFJL010000318 DAZFJL010000000
VERSION     DAZFJL010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.321"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..622
                     /locus_tag="ACHJ8C_004793"
     CDS             <1..622
                     /locus_tag="ACHJ8C_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFO5554475.1"
                     /translation="WVVSDLHGCYTNLMKKLETIGFDTKKDLLISVGDLVDRGTENVE
                     CLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWMLNGGGWFFNLDYDKEILAKALAH
                     KADELPLIIELVSKDKKYVICHADYPCDEYEFGKPVDHQQVIWNRERISNSQDGIVKE
                     IKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQGEGA"
     gene            619..>1024
                     /locus_tag="ACHJ8C_004794"
     CDS             619..>1024
                     /locus_tag="ACHJ8C_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO5554476.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDISKTEQ"
BASE COUNT          305 a          223 c          264 g          232 t
ORIGIN      
        1 ttgggtagtt agcgatctgc acggatgcta cacgaacctg atgaaaaaac tggagacgat
       61 aggattcgac accaaaaaag acctgctcat ctcggttggc gatttggttg atcgcggtac
      121 agagaacgtc gaatgtctgg aattaatcac attcccctgg ttcagagctg tgcgtggaaa
      181 ccatgagcaa atgatgattg atggcttatc agagcgtgga aacgtcaatc actggatgct
      241 taatggcggt ggctggttct ttaatctcga ttacgacaaa gaaattctgg ctaaagctct
      301 tgcccataaa gcagatgaac ttccgttaat catcgaactg gtgagcaaag ataaaaaata
      361 tgtcatctgc cacgccgatt atccttgtga cgaatacgag tttggaaagc cagttgatca
      421 tcagcaggta atctggaacc gcgaacgaat cagcaactca caagacggga tcgtaaaaga
      481 aatcaaaggc gcggacacgt tcatctttgg tcatacgcca gcagtgaaac cacttaaatt
      541 tgccaaccag atgtatatcg atactggggc agtgttctgc ggaaatctca cattgattca
      601 ggtacaggga gaaggcgcat gagactcgaa agtgtagcta aatttcattc gccaaaaagc
      661 ccgatgatga gcgactcacc acgggccacg gcttctgact ctctttccgg tactgatgtg
      721 atggctgcta tggggatggc gcaatcacaa gccggattcg gaatggctgc attctgcggt
      781 aagcatgaac tcagccagaa cgacaaacaa aaggctatca actatctgat gcaatttgca
      841 cacaaggtat cggggaaata ccgtggtgtg gcaaagctcg aaggaaatac taaggcaaag
      901 gtactgcaag tgctcgcaac attcgcttat gcggattatt gccgtagtgc cgcgacgccg
      961 ggcgcaagat gcagagattg ccacggtaca ggccgtgcgg ttgatatatc aaaaacagag
     1021 caat
//
LOCUS       DAZFJL010000319         1024 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFJL010000319 DAZFJL010000000
VERSION     DAZFJL010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.322"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..622
                     /locus_tag="ACHJ8C_004795"
     CDS             <1..622
                     /locus_tag="ACHJ8C_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFO5554477.1"
                     /translation="WVVGDLHGCYTNLMNKLDTIGFDNKKDLLISVGDLVDRGAENVE
                     CLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFFNLDYDKEILAKALAH
                     KADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIWNRERISNSQDGIVKE
                     IKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQGEGA"
     gene            619..>1024
                     /locus_tag="ACHJ8C_004796"
     CDS             619..>1024
                     /locus_tag="ACHJ8C_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO5554478.1"
                     /translation="MRLESIAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAVFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEL"
BASE COUNT          300 a          224 c          265 g          235 t
ORIGIN      
        1 ttgggtagtt ggcgatctgc acggatgcta cacgaacctg atgaacaaac tggatacgat
       61 tggattcgac aacaaaaaag acctgcttat ctcggtgggc gatttggttg atcgtggtgc
      121 agagaacgtt gaatgcctgg aattaatcac attcccctgg ttcagagctg tacgtggaaa
      181 ccatgagcaa atgatgattg atggcttatc agagcgtgga aacgtcaatc actggctgct
      241 taatggcggt ggctggttct ttaatctcga ttacgacaaa gaaattctgg ctaaagctct
      301 tgcccataaa gcagatgaac ttccgttaat catcgaactg gtgagtaaag gaaaaaaata
      361 tgtcatctgc cacgccgatt atccttgtga taaatacgag tttggaaagc cagttgatca
      421 tcagcaggta atctggaacc gcgaacgaat cagcaactca caagacggga tcgtgaaaga
      481 aatcaaaggc gcggacacgt tcatctttgg tcatacgcca gcagtgaaac cactcaaatt
      541 tgccaaccag atgtatatcg atactggcgc agtgttctgc ggaaacctca cattgattca
      601 ggtacaggga gaaggcgcat gagactcgaa agcatagcta aatttcattc gccaaaaagc
      661 ccgatgatga gcgactcacc acgggccacg gcttctgact ctctttccgg tactgatgtg
      721 atggctgcta tggggatggc gcaatcacaa gccggattcg gaatggctgt attctgtggt
      781 aagcacgaac tcagccagaa cgacaaacaa aaggctatca actatctgat gcaatttgca
      841 cacaaggtat cggggaaata ccgtggcgtg gcaaagcttg aaggaaatac taaggcaaag
      901 gtactgcaag tgctcgcaac attcgcttat gcggattatt gccgtagtgc cgctacgccg
      961 ggcgcaagat gcagagattg ccacggtaca ggccgtgcgg ttgatatagc caaaacagag
     1021 ctgt
//
LOCUS       DAZFJL010000320         1021 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFJL010000320 DAZFJL010000000
VERSION     DAZFJL010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.323"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            111..>1021
                     /gene="tdcA"
                     /locus_tag="ACHJ8C_004797"
     CDS             111..>1021
                     /gene="tdcA"
                     /locus_tag="ACHJ8C_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO5554479.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGC"
BASE COUNT          284 a          193 c          235 g          309 t
ORIGIN      
        1 acgatgccat ggaaagtatt gaaagttaat ttgtgagtgg tcgcacatat cctgttcatt
       61 tcattttgat acacttcata ccgtcaatga ggtaattaac gtaggtcgtt atgagcacta
      121 ttcttcttcc gaaaacgcag cacctggtag tctttcagga agttattaga agtggttcta
      181 tcggctcggc tgcaaaagag ttagggttaa ctcaaccggc cgtcagtaaa atcattaacg
      241 atattgaaga ttattttgga gtggagttag tggtgcggaa aaataccggt gtaacattaa
      301 cacctgccgg tcaattgtta ctctcccgtt ccgaatccat tacccgtgaa atgaaaaata
      361 tggttaatga gataagcggt atgtcttctg aggcggtggt ggaagtctca tttggttttc
      421 cttcattgat tggttttact tttatgtcag ggatgatcaa caagttcaaa gaggtgttcc
      481 cgaaagcgca ggtttctatg tatgaagcgc aactgtcttc gttcttaccg gcaatccgcg
      541 acggtcgact ggattttgcg attggtacgt taagcgcaga aatgaagctt caggatttac
      601 atgttgagcc gctgttcgag tccgagtttg tgctggtagc cagtaagtcc cgaacatgca
      661 ccggcaccac cacgctggag tcgttgaaga acgaacagtg ggtgttgcca caaaccaata
      721 tggggtacta cagcgaacta cttactacgt tacaaagaaa tggcatcagt attgaaaaca
      781 tcgttaaaac cgactcagtc gtgacaattt ataatcttgt tctcaatgct gatttcttaa
      841 ctgtaattcc ttgtgatatg acatcacctt ttggttctaa tcaatttatt actattccgg
      901 ttgaagaaac attacctgtg gcacaatatg ccgcggtatg gtcgaaaaat tatcgtatta
      961 aaaaagcagc atcggttttg gtggaattag ccaaagagta ttcatcttat aatgggtgta
     1021 g
//
LOCUS       DAZFJL010000321         1017 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFJL010000321 DAZFJL010000000
VERSION     DAZFJL010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.324"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..620)
                     /locus_tag="ACHJ8C_004798"
     CDS             complement(<1..620)
                     /locus_tag="ACHJ8C_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5554480.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADAIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGSLKQRATGERLHKLISTHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPGCVVPKLNSSWEPVYQVTFPDGSTDVLT
                     QEDIWHVRTLTLDGLVGLNPVAYAREAISLAAATEEHGARLFSNGAV"
     gene            complement(620..802)
                     /locus_tag="ACHJ8C_004799"
     CDS             complement(620..802)
                     /locus_tag="ACHJ8C_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554481.1"
                     /translation="MFLIILAPLVGVLGALLLAYGAWLIYPPAGFVVAGVLCLLWSWL
                     VARYLDRTQSSVGGGK"
     gene            complement(814..>1017)
                     /locus_tag="ACHJ8C_004800"
     CDS             complement(814..>1017)
                     /locus_tag="ACHJ8C_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO5554482.1"
                     /translation="PVMNWMMSNVTVKPDKNDNIFPNKSTPEAKIDGPVAMFTAMSRM
                     LVNGGEPEPDLSEHLVSVGIRSL"
BASE COUNT          235 a          319 c          260 g          203 t
ORIGIN      
        1 accgcaccat tgctgaacag cctggccccg tgctcttcgg tcgccgctgc cagcgatatt
       61 gcctcgcggg cataggcgac gggattcagc cccaccagac cgtccagcgt cagcgtgcgc
      121 acatgccaga tatcctcctg ggtcagcaca tccgtggaac cgtccgggaa tgtgacctga
      181 tagaccggct cccagctact gttaagcttc ggcaccacac agccaggatc gacgggcagc
      241 agttcagcca cttcgccaaa tgctttcact ttgtaagcat aaaagtttcc ccgcaggcac
      301 agacaggtga ccaccagctc ccagaactcc tgcggcgtca tatagccatt gggatgcgtg
      361 gagatcagct tatgcagacg ttcgccggtg gctctctgtt tcaggctgcc gttcaggtga
      421 tacagattgc agggcaacat cccgaccgac tctgccagca ccctgacgca ggaaaaaacc
      481 gccgtcagtc gcatggcccg ctggctgctg atctgctttc cggtataggt gtcatatgac
      541 agcccgatag catccgccag ttctgctggc gtggtcaccg gcgcgtcact ttttcgttga
      601 aataatcccg aaaagaacac tatttacctc cgccgacaga cgactgtgta cggtcgagat
      661 atcgcgccac cagccacgac cagagcaggc acagcacccc ggcaacaaca aaacccgccg
      721 ggggataaat cagccaggca ccatacgcca gcaaaagcgc acccagcacg cccaccagtg
      781 gcgcgagaat tatcagaaac ataatgacct cggttaaagt gagcggatgc ccacgctgac
      841 cagatgttca gacagatccg gctccggttc accaccattg accagcatcc ggctcatcgc
      901 tgtaaacatc gcaacagggc cgtcgatttt ggcttccggc gtggatttat tcgggaagat
      961 attgtcgttt ttgtccggtt ttaccgtaac gttagacatc atccagttca tgaccgg
//
LOCUS       DAZFJL010000322          982 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFJL010000322 DAZFJL010000000
VERSION     DAZFJL010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.325"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(1..639)
                     /locus_tag="ACHJ8C_004801"
                     /pseudo
     CDS             complement(1..639)
                     /locus_tag="ACHJ8C_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleH"
     gene            complement(803..913)
                     /locus_tag="ACHJ8C_004802"
                     /pseudo
     CDS             complement(803..913)
                     /locus_tag="ACHJ8C_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          290 a          247 c          159 g          286 t
ORIGIN      
        1 ttatccattc taataccaat aatatctcta ttatcgccat atattttctc tgcactcccg
       61 gcaccataat actggttgaa acaacgaact tcgcttgtca cctcttcatg gctttgagat
      121 atagtaaaca tcttcaacac ttttgttgta ttttccatat cttcatacac gacagcatta
      181 ccacctttac caataacgtt acctagcacg ggtgatttgt tgttgctaag cctcagcaac
      241 gctgactgag tataatccgc tggcggtaac tccggctgcg cataatccac tggaggtaaa
      301 tcaggtctgt tcgaacgaac agatcctctt tctatagtcg tgctcactgt cgatgaattc
      361 agcatagcct caatttttct ggtgatctct cccttaggcc atcccagtct atacagcata
      421 ttagtaaaac atccactatg actctctctt gtcacgcaaa acctattatc acttaaaaca
      481 acgcgatatg ttctgttgct aacctttact tgcgccccat tatcagagtg agcggcagca
      541 tcccttacag agggataaaa cacgattatc aggcaaagtc aggtttctgg ttaaagaatt
      601 ccatgaacac cccaaattta cagaagatgg tgatagcata catttcaacc ttcaaaatga
      661 atcaatcttt actttcttaa caaacatcac catgacatga caacaaaaaa ctggaaatta
      721 actattgttt ttgagtatct ggcaactcat tcatccttcc agcatcccgt cagacgaacg
      781 tttacgaact ataaatcagg tattactggc cctcgaaatc tggagatact gacgtatcaa
      841 cacggttcag taacacccga tacctcttcc agactgtcaa caacgatgtc tcttcatccg
      901 ttgcgatctg cagcatcctg aatatgaatg ccatagctta cggcatcacg aaaagtttat
      961 gagcggctca aagcatcaag ta
//
LOCUS       DAZFJL010000323          970 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFJL010000323 DAZFJL010000000
VERSION     DAZFJL010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.326"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..399)
                     /locus_tag="ACHJ8C_004803"
     CDS             complement(<1..399)
                     /locus_tag="ACHJ8C_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO5554483.1"
                     /translation="MTESAILTAYNAIDAASVALGRPCLTLPEREHFQAERDVATRNL
                     STLSRQYRDEVGVLSSQLALPRNTQVDRVIYGINCTDLSLADRGRAAGLVSQFGSFSF
                     TVAHAFCRLVYQLGVKNALMALESAHRFAFF"
     gene            514..696
                     /locus_tag="ACHJ8C_004804"
     CDS             514..696
                     /locus_tag="ACHJ8C_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014655995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554484.1"
                     /translation="MKRKAIAITESTYLDITRIALEISQKTGQIVTWSEVVHFMIKNY
                     LNDAKQDMIHQRNQKK"
BASE COUNT          322 a          204 c          235 g          209 t
ORIGIN      
        1 aaagaaagcg aaacggtgcg cagactcaag cgccatcagc gcatttttca caccgagctg
       61 ataaaccaga cggcagaaag cgtgcgccac ggtgaaggaa aacgaaccaa actgtgatac
      121 cagtccggcg gccctacccc tgtcagcgag agataaatcc gtgcagttaa ttccgtaaat
      181 cacacggtca acctgcgtgt tacgtggcag ggcaagctgt gaagagagca ctccgacctc
      241 atcacgatac tggcgggaaa gcgtggaaag gttgcgggtg gcaacatcgc gctcagcctg
      301 aaaatgctca cgttcgggca gtgtcagaca aggacgaccg agagcgacgg atgccgcgtc
      361 gatggcgtta tacgctgtga ggatggctga ttcagtcata aaaacgaata aaaaacgcac
      421 tggtgttaaa gtgttaacag gttaacactc ataattttta tgggtcaagt gaaaatgttg
      481 tcttgttaaa ctgttaacaa tggagatggg cgaatgaaaa gaaaggcaat agcaattaca
      541 gaatcaacat atctggatat tactagaata gcccttgaga taagccaaaa aacagggcaa
      601 atagttacat ggtcggaagt tgtacacttt atgataaaaa actacttaaa cgacgcaaaa
      661 caagacatga tccaccaaag aaaccaaaaa aaataacatc gtagttacct atagatacga
      721 agattgctaa gaagcaatat gcagacaacc agtgacttaa ttcttgacct gctgataaag
      781 cattttgttg tggatagcat tacaggcatg acgatgttcg gcctgataac gcttgttctt
      841 ccgttggtga tgaccataca cgacaaaatg aacaggcgca cagactcctt ttaagattca
      901 gtgcgcacaa tgacgttatg tgtaaaaagg ccgctgcggt ggaaagtatc gcagcggcct
      961 tttcaacata
//
LOCUS       DAZFJL010000324          956 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFJL010000324 DAZFJL010000000
VERSION     DAZFJL010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.327"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(1..128)
                     /locus_tag="ACHJ8C_004805"
                     /pseudo
     CDS             complement(1..128)
                     /locus_tag="ACHJ8C_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleH"
     gene            complement(202..>956)
                     /gene="nleC"
                     /locus_tag="ACHJ8C_004806"
     CDS             complement(202..>956)
                     /gene="nleC"
                     /locus_tag="ACHJ8C_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HFO5554485.1"
                     /translation="LDALSRSQTFRDAVSYGIHNEKVHIGCIKYRNEYELNEESSVKI
                     DDIQSLTCNELYEYDVGQEPIFPICEAGENDNEEPYVSFSVAPDTDSYEMPSWQEGLI
                     HEIIHHVTGSSDPSGDSNIELGPTEILARRVAQELGWSVPDFKGYAEPEREAHLRLRN
                     LNALRQAAMRHEENERAFFERLGTISDRYEASPDFTEYSAVSNIGYGFIQQHDFPGLA
                     INDNLQDANQIQLYHGAPYIFTFGDVDKHNQR"
BASE COUNT          263 a          218 c          183 g          292 t
ORIGIN      
        1 tttccgccat gataagattg cattatttga ctttcactcc atgaacggtc ggaaacatta
       61 taagatgata tatctattgg attaaactca ttcttcgcgc ggtcatataa gacatttgtc
      121 tctgttgtat gttgcacgat tacccatcat caactccgtt aaaatgtatg aatagtaacc
      181 ttatgtcact gcaaagacga atcatcgctg attgtgtttg tccacatccc caaatgtaaa
      241 aatataaggg gcgccatgat acagttggat ctgatttgca tcctgtaaat tatcgttgat
      301 agccaatcca ggaaaatcat gttgctggat aaatccgtat cctatgttag acacagcgga
      361 atactctgtg aaatcaggac tcgcctcata tcggtcactg atcgtaccca gcctttcgaa
      421 gaaagccctc tcattctctt catgcctcat ggcagcctgt cgaagggcat tcaggttacg
      481 taacctaaga tgagcttcac gttctggctc tgcatatcct ttgaagtcgg gaacactcca
      541 tcccagttct tgagcgacac gacgtgcgag aatctcggtg ggtcctaact ctatattact
      601 atctccagat ggatcgctag atccagtaac atgatgaata atctcgtgaa tcagtccttc
      661 ctgccacgat ggcatctcat aagagtcagt atctggcgca acactaaaac tgacataagg
      721 ctcttcgtta tcgttttctc ctgcttcgca aatggggaaa attggctctt gcccgacatc
      781 atattcatat aattcgttac aggttagtga ttgaatatca tcaattttga cagaagattc
      841 ttcgttaagc tcgtattcgt ttctgtattt aatgcaacca atgtgtacct tctcattatg
      901 aatgccatag cttacggcat cacgaaatgt ctgtgatcgg ctcaaagcat caagta
//
LOCUS       DAZFJL010000325          955 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFJL010000325 DAZFJL010000000
VERSION     DAZFJL010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.328"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            40..864
                     /gene="gadX"
                     /locus_tag="ACHJ8C_004807"
     CDS             40..864
                     /gene="gadX"
                     /locus_tag="ACHJ8C_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO5554486.1"
                     /translation="MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQV
                     DKCVENFVLVSRDTLSLFLPMLKEEALKLHVHKKVPSLLVHHCTRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVFCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQELPNCGPAASMAAQGIFYGTDRSAEGIRL"
BASE COUNT          282 a          176 c          211 g          286 t
ORIGIN      
        1 taataaacag taatatgttt atgtaatatt aagtcaacta tgcaaccact acatggtaat
       61 tgtctaattg cgtacgcaag acataaatat attctcacca tggttaatgg tgaatatcgc
      121 tattttaatg gcggtgatct ggtttttgcg gatgcaagcc aaattcaagt ggataagtgt
      181 gttgagaatt tcgtattagt ttcaagagac acgctttcat tatttctccc gatgctcaag
      241 gaggaggcat taaaacttca tgtacataaa aaagttcctt cattactcgt tcatcactgt
      301 accagagata ttcctgtttt tcaggaagtt gcgcaactat cgcagaataa gaatcttcgc
      361 tatgcagaaa tgctacgtaa aagggcatta atctttgcgt tgttatctgt ttttcttgag
      421 gatacgcagt ttataccgtt acttctgaac gtgttgcaac cgaatatgcg aacacgagtt
      481 tgtacagtca tcaataataa tatcgcccat gagtggacat tagcccgaat cgctagcgaa
      541 ttgttgatga gtccaagcct gttaaagaaa aaattgcgcg aagaaggtac gtcatattca
      601 cagttgctta ctgagtgtag gatgcagcgt gctttacaac ttattgttat atatggggtt
      661 tcaattaagc gagttgcagt attctgtgga tatcacagcg tgtcgtattt catttacgtt
      721 tttcgaaatt attatgggat gacgcccaca gagtatcagg agcgatcggc gcaggaatta
      781 ccgaactgtg gcccggcggc aagtatggct gcgcaaggga ttttttacgg cactgaccgt
      841 tctgcggaag gaataagatt atagagtttt actcagacat aaaaaaaacc cggcataggg
      901 gaccgggaag aggatagtct gccgtctcca gactaataaa ccgttataac actcc
//
LOCUS       DAZFJL010000326          902 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFJL010000326 DAZFJL010000000
VERSION     DAZFJL010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.329"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..421)
                     /gene="escT"
                     /locus_tag="ACHJ8C_004808"
     CDS             complement(<1..421)
                     /gene="escT"
                     /locus_tag="ACHJ8C_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HFO5554487.1"
                     /translation="MNEIMTVIVSSFYCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIVLKEISIGFFIGLSFTILFWAIDAAGQIIDT
                     LRGSTISSIFNPSISDSSSITGVILYQFISVIFVIHGG"
     gene            complement(421..690)
                     /gene="escS"
                     /locus_tag="ACHJ8C_004809"
     CDS             complement(421..690)
                     /gene="escS"
                     /locus_tag="ACHJ8C_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFO5554488.1"
                     /translation="MDTGYFVQLCVQTFWIIFILSLPTVIAASVIGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            complement(690..>902)
                     /gene="escR"
                     /locus_tag="ACHJ8C_004810"
     CDS             complement(690..>902)
                     /gene="escR"
                     /locus_tag="ACHJ8C_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFO5554489.1"
                     /translation="FTMGQLEAAFKIGFLLYLPFIAIDLIISNILLALGMMMVSPVTI
                     SIPFKILLFILVGGWQKLFEFLLVVN"
BASE COUNT          335 a          174 c          155 g          238 t
ORIGIN      
        1 tcccaccatg aataacaaag atcacagaga taaattggta caaaataacg ccagtgatag
       61 aagatgaatc acttatggac gggttaaaaa ttgaagatat tgttgaacct cttagagtat
      121 caataatctg cccagccgca tctattgccc aaaaaagaat agtaaatgat aacccaatga
      181 aaaaaccaat acttatttct tttagcacta ttcctgtcaa ctgaaagatt ccagatggca
      241 atttttccga aaatgtatag ttctctacta tgatcggaag cgtaaacgca atcataattg
      301 agttgcgaat gaagttactg agtaaaacac ctgttgaaaa tattggtagg ataataaaca
      361 tccccagcgg cctgagtata caataaaatg atgatactat gaccgtcatt atttcattca
      421 ttagccgttc accttcggaa tcatttcaaa tattatcgac gagaagttga tgattgttgt
      481 tcccatccag tgataagtca gggcaagcgt agcaaacact gctattattt ttagcaaaaa
      541 aggcaatgtt tgatcctgta actgcgttat agcctggacc agactaataa taataccgat
      601 aacagaggcc gctatgactg taggcaatga gaggataaat attatccaga acgtttgcac
      661 acataattga acaaaatatc cagtatccat caattcacca ccaacagaaa ttcgaataat
      721 ttttgccagc ctccaacaag aatgaataac aagatcttaa aaggaatcga aattgttact
      781 ggcgatacca tcatcatacc caaggccaat aagatattgg aaatgatcaa atctatcgca
      841 ataaagggta aatagagcaa aaaacctatc ttgaatgcag cttcaagctg ccccatcgtg
      901 aa
//
LOCUS       DAZFJL010000327          895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZFJL010000327 DAZFJL010000000
VERSION     DAZFJL010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.330"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(20..424)
                     /locus_tag="ACHJ8C_004811"
     CDS             complement(20..424)
                     /locus_tag="ACHJ8C_004811"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554490.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LQLIKCQNSDSEIWFSGIDIDIDIDIDIDTQILFHTAEVDAFSSSNGVSIINGVILND
                     VSWNVVVESTEVNSLNLFVWRFFERYVVSSFL"
     gene            complement(616..>895)
                     /gene="traM"
                     /locus_tag="ACHJ8C_004812"
     CDS             complement(616..>895)
                     /gene="traM"
                     /locus_tag="ACHJ8C_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFO5554491.1"
                     /translation="SATASMLLELGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQS
                     SVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSVEMERFFPKNDDE"
BASE COUNT          293 a          198 c          128 g          276 t
ORIGIN      
        1 caactaaact ccttgccctt tataaaaagc tactaactac gtatctttcg aagaatctcc
       61 agacaaacaa attcaagcta ttcacttccg tactttcaac caccacattc catgatacat
      121 cattcaatat tacaccatta ataatactta ctccattcga ggacgagaaa gcatcaactt
      181 cagcagtatg aaataaaatt tgcgtatcaa tatcaatatc aatatcaata tcaatatcaa
      241 taccagaaaa ccatatttca gaatcactat tctgacactt tattaattgt aaaaaaacag
      301 agtttgagtc tatgaatttt cctgactctg tgcggacaca gactggatat tttgcggata
      361 aaataatttc cggcaggcct gattgacgta tgagtgctct ttctctagta tccatcgcac
      421 acataggaac ctcctcaaag gattctatgg acagtcgatg cagggaggga caagctccct
      481 gcatcggcga ttttcaagtt caaattttaa cgtggcatta accacgtaat taatatacta
      541 attattctta ttattgtcac cagtttgaac cattaaaaat tctgatattt gaacaaagtc
      601 aaaatttatt tccctttatt catcatcatt ttttggaaag aacctttcca tctcaaccga
      661 caccttctct ctgatatctt caaccatatt ggcatattca aactttggat ttcctgagac
      721 atgaggactg agagactcaa tacccaaaat tttcgctact gatgactgtg tttttacaac
      781 gcattcaaga agtagtttat taaactcggc ctgattaaat gcagactctt tgcgctccat
      841 ctgagcctca tatacacgaa gacccagttc aagaagcatt gaagccgttg ctgaa
//
LOCUS       DAZFJL010000328          885 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFJL010000328 DAZFJL010000000
VERSION     DAZFJL010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.331"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            411..740
                     /locus_tag="ACHJ8C_004813"
     CDS             411..740
                     /locus_tag="ACHJ8C_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554492.1"
                     /translation="MKTLTFNNGTVSVGDVFVSSWGYEQTNVNFYQVISVHGKKTVTV
                     QEIRASVHRTHSMSGYKTPLLNDFCGEPLKRRVRDYYSTPAIEIEEFETAYKGSPEEK
                     HEFTSYY"
BASE COUNT          234 a          202 c          216 g          233 t
ORIGIN      
        1 gctcacacgc gcagtacagc gccccgccct gctgcaatgc accaggacgg cgtaaactcg
       61 ctggcgctcg cgctaactgc ggcattctgc ggcgcaaatc agcatgatca tggcctgttg
      121 gtctgttctg gctttgcagg gcgaagggat ggccctgtgc atcctggtat gttcgtcagc
      181 ggctgcgccg cgcttcgggg cgatgtggtc ccgctgctcc ctggtatttt ctttcgcccc
      241 ttctatgccc tgtcgggcat tacatgaatc taaccatctg tatttgatgg tatttatttt
      301 aaagaaagga ggaaaaaaca cttgataata ggtcagttct gacctattat aaacacatcg
      361 ggaggggatg gtccccaacc accaaagccg ctcagcggca ggagataaaa atgaaaacat
      421 taacttttaa taacggcact gtttctgttg gcgatgtgtt tgtatcttcc tggggatatg
      481 agcaaacgaa cgttaatttc taccaggtta tttctgttca tggcaaaaaa accgtaactg
      541 ttcaggagat tcgcgcttca gttcatcgga ctcattcaat gagcggatat aagaccccat
      601 tacttaatga tttctgtggt gagccattaa aacggcgagt gcgtgattat tacagtacgc
      661 cagcaattga aattgaggaa ttcgaaacag catataaagg atcaccggaa gaaaaacacg
      721 agttcacatc atactactaa gaaaatagtt gcagggggta tcccctgcaa ccatgttaaa
      781 ttgttaatag tgaatacagg ggcttaatcg cctctttttt atttggtgcg cagtaaaact
      841 ccgtccttca aggcgtggag aatgccaggc gaatgcagca atgta
//
LOCUS       DAZFJL010000329          883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZFJL010000329 DAZFJL010000000
VERSION     DAZFJL010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.332"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>883
                     /locus_tag="ACHJ8C_004814"
     CDS             <1..>883
                     /locus_tag="ACHJ8C_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_109867760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO5554493.1"
                     /translation="ARGSLLALFNLSLYGAFLSASQAAQLNIDNVWARDYLDLAQNKG
                     VFKAGATNVSIQLKNGQTFNFPNVPIPDFSPASNKGATTSIGGAYSVTATHNGTTHHA
                     ISTQNWGQSSYKYIDRMTNGDFAVTRLDKFVVETTGVKNSVDFSLNSHDALERYGVEI
                     NGEKKIIGFRVGAGTTYTVQNGNIYSTGQVYNPLLLSASMFQLNWDNKRPYNNTTPFY
                     NETTGGDSGSGFYLYDNVKKEWVMLGTLFGIASSGADVWSILNQYDENTVNGLKNKFT
                     QKVQLNNNTMSLNSDSFT"
BASE COUNT          286 a          152 c          180 g          265 t
ORIGIN      
        1 ccgctcgcgg cagcctttta gcattattta atctatcatt gtatggtgct tttttaagcg
       61 catctcaggc tgctcaacta aatattgata atgtatgggc tagagattat ttagacctcg
      121 cacaaaataa gggggtgttt aaagctggtg cgaccaatgt ttcaattcaa ctcaagaatg
      181 gccagacgtt taattttcca aatgttccaa ttcctgattt ctcgccggcc tcaaataaag
      241 gcgctactac atctataggt ggagcttata gtgtcacagc aacccataac ggaacaactc
      301 atcatgcaat aagcacccaa aactggggac aaagctcata taaatatata gaccggatga
      361 cgaatggaga ttttgctgta acacgacttg ataagtttgt tgttgaaaca acaggggtaa
      421 aaaattcagt agatttttct ctcaatagtc atgatgctct tgaacgttat ggtgtggaga
      481 tcaatggtga gaaaaaaatc attggtttca gggttggggc cgggacgact tataccgttc
      541 aaaatggtaa tatatatagt acaggacagg tatacaatcc tcttttgtta agcgcttcaa
      601 tgtttcagtt aaactgggat aacaaaagac catataataa cacgacacct ttttataatg
      661 aaactaccgg tggagacagt ggttccggtt tctatctgta tgataacgta aaaaaagaat
      721 gggttatgct tggtacttta tttggaatag catccagtgg tgcagatgtt tggtctattc
      781 tgaatcagta tgatgaaaat acagttaatg gtttaaaaaa caaatttact caaaaagtcc
      841 agttaaacaa taatacaatg tcgcttaata gtgacagttt tac
//
LOCUS       DAZFJL010000330          871 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFJL010000330 DAZFJL010000000
VERSION     DAZFJL010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.333"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            154..>871
                     /locus_tag="ACHJ8C_004815"
     CDS             154..>871
                     /locus_tag="ACHJ8C_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5554494.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     YGPQLISCTNNNMLIRCLRQDTSIDIECHDILLSAENAEELLVQRKTDLAITLQPVIS
                     RSVICMPLHTIRNSLICSNKHPRITDTSNYEQIIAEEFTLLISKSAGIDEIQMDIDER
                     FINRKVSFRSSSLLTIINSIS"
BASE COUNT          294 a          176 c          139 g          262 t
ORIGIN      
        1 cgcatgaatt gctcgtaacc cacctgacag ctatgctatt ttagataaac ttaatccaaa
       61 gttaagcaga aaaacataat acatataaat acaacagcaa cactattgat caagtgaatg
      121 atttatgaaa gagataatat aacggagtat gttatggcta atctttatga tctaaaaaaa
      181 tttgacctca atttactggt catatttgaa tgtatttatc aacatctcag cattagtaaa
      241 gcggcagaat cattatatat aacgccctct gctgttagtc agtcgttaca acgtttacgt
      301 gcgcaattta atgacccgtt attcattcgc tcagggaaag gtatcgcgcc gactacaaca
      361 gggttaaacc tgcatcacca tcttgagaaa aatctcagag gccttgaaca aacgatcaat
      421 atcgttaata agtctgaact aaagaaaaat tttattatct acggtcctca gcttatttct
      481 tgtactaata acaacatgct aatccgctgt cttagacaag atacttcaat cgatatagaa
      541 tgccatgaca tcctgctttc agctgaaaat gccgaagaac tgttggttca acgtaaaact
      601 gacctggcaa ttacgctgca accagtaatt agtcgctctg tcatctgtat gccgttacac
      661 actattcgca attcccttat atgtagcaac aaacatcctc gtattactga taccagtaac
      721 tatgaacaaa ttatcgccga agaattcacc ctgttgatat ctaaatcagc agggattgac
      781 gaaattcaaa tggacataga tgaacgtttt ataaaccgta aagtttcatt tcgcagttct
      841 tctttgttga ctataattaa tagcatttct g
//
LOCUS       DAZFJL010000331          849 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFJL010000331 DAZFJL010000000
VERSION     DAZFJL010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.334"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..218
                     /locus_tag="ACHJ8C_004816"
     CDS             <1..218
                     /locus_tag="ACHJ8C_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EscG/YscG/SsaH family type III secretion system
                     needle protein co-chaperone"
                     /protein_id="HFO5554495.1"
                     /translation="KLPKYAEAILNVFPQIIPDKKDIAHLEFIILFGLNRKNDAIKAL
                     EGCMDDESSQLLYSLFHENVSGWVRGF"
     gene            303..>849
                     /gene="espF"
                     /locus_tag="ACHJ8C_004817"
     CDS             303..>849
                     /gene="espF"
                     /locus_tag="ACHJ8C_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HFO5554496.1"
                     /translation="MLNGISNAASTLGRQLVGIASRVSSAGGTGFSVAPQAVRLTPVR
                     VHSPFSPGSSNVNARTIFNVSSQVTSFTPSRPAPPPPTSGQASGSAGLPPIAQALKNH
                     LAAYELLKISETSSFKPVRPAPPPPTSGQTSGSAGLPPIAQALKNHLAAYEQSKISET
                     SGFKPARPAPPPPTSGQASGSA"
BASE COUNT          224 a          187 c          198 g          240 t
ORIGIN      
        1 ataagcttcc caaatatgcg gaagctatcc tgaatgtatt cccacaaatt atacctgata
       61 aaaaagatat cgcacattta gaatttatta tcttatttgg attaaataga aaaaatgatg
      121 cgataaaggc tttggagggc tgtatggatg atgagtcgag tcagttattg tacagtttgt
      181 tccatgagaa tgttagtggt tgggtacgag gattttaatt tattttgatt taaaaatcat
      241 tttattattc gttttgatat atatgagagt tagccaagat tagatataaa gaggcataaa
      301 ttatgcttaa tggaattagt aacgctgctt ctacactagg gcggcagctt gtaggtatcg
      361 caagtcgagt gagctctgcg gggggaactg gattttctgt agcccctcag gctgttcgtc
      421 ttactccggt gagagttcat tccccctttt ctccaggttc gtcgaatgtt aatgcgagaa
      481 cgatttttaa tgtgagtagc caggtgactt catttactcc ctctcgtccg gcaccgccgc
      541 caccgacaag tggacaggca tccgggtctg ccgggctacc gcccattgcg caggcattaa
      601 aaaatcattt agctgcctat gagctattga aaatttctga gacttcaagc tttaagccag
      661 tccgtccggc accgccgcca ccaacaagcg gccagacatc tgggtctgct gggctaccgc
      721 ccattgcaca ggcattaaaa aatcatttag ctgcctatga gcaatcgaaa atttctgaga
      781 cttcgggctt taagccagcc cgtccggcac cgccgccacc gacaagtgga caggcatccg
      841 ggtctgccg
//
LOCUS       DAZFJL010000332          836 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFJL010000332 DAZFJL010000000
VERSION     DAZFJL010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.335"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
BASE COUNT          246 a          174 c          139 g          277 t
ORIGIN      
        1 tcaaattctt tagttatttt ttcttttaat tccatacgtg acaatctcat agaatcagaa
       61 aaaactgatt gcgagcccaa cggttttatc ggcgcaggca aagacacatc tcgtcctgcc
      121 cccccccctg cattacgatt tataaatgtg gcaagagtcg acttagaagg ccgaggaggt
      181 tcgggtggag gaagtgtccg ttctgccata aattccctgt gagtcattat agtcgtaaaa
      241 ggagatgctg gatgttttac ctgtacggac ggagatgtcg ccgcctctga tgttatgttt
      301 aacaaagaca attgtcgatt ttacatttag taaatgtgcc gaacttgctg tatggcgtga
      361 taaaccatct gatatttttt gtttgcctgg gggcaagcta tactctcact gctgagaacg
      421 gttatttctc tcccggaagc cattttttat tatcaccacg tctgagttta caacaatctc
      481 cccaaaacgc atttctaatc gcacttacaa tattatttat tgctttagac aaagacacat
      541 taccaatcca caaaacctgt tttttgttaa ttaaagcatt cattttgaaa ttgtttgtat
      601 tagtaaaaaa caaaatcacc atttctttta ttgatattat tcaagctgca tcttactaaa
      661 agccatctca tccctgataa acacatcttc cactcatact tctattaggg tgcatgtgca
      721 tttacttatt attatagtga actatctatt ctatagtagt caactaaatt tggtcacctg
      781 attaaagata atgttttccc caccccaaca taaaacaggt gacttagtaa ataata
//
LOCUS       DAZFJL010000333          831 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZFJL010000333 DAZFJL010000000
VERSION     DAZFJL010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.336"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(140..>831)
                     /locus_tag="ACHJ8C_004818"
     CDS             complement(140..>831)
                     /locus_tag="ACHJ8C_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO5554497.1"
                     /translation="LPNTVNLSSLTYNKEDDFLYATQNSPAQLLKITKSGDIMDRAPL
                     PFISDAETIEHIQGNIFAAVDEKTSELFFFTVTKDMHISFRNKIQLEKFNKKNRGFEG
                     LAWKADDRMLFVAKERRPSKIFIYQLSPDLLSVKQATIPEALNDIRVNDISGLAFNNE
                     SLMILSDESRKLLKFNLTEMSFIEMLDLTKGNHSLTSDLPQPEGIVTLPDESIYVASE
                     PDILAKFVPNK"
BASE COUNT          234 a          146 c          165 g          286 t
ORIGIN      
        1 tgagtaagat tctggttgat aatagctgat gtccttttca gctattaatg tgtttattta
       61 gcgtgaaatt atactcgtat ttttataact ttatgagatt gccagatttg gtcgataaat
      121 aataaggcca gtgacagact tacttattag ggacaaattt cgcgaggata tcaggttcac
      181 tggcaacata aatgctttca tcaggcagag ttacaattcc ttcaggttgg ggcaaatcac
      241 tggtcagaga atgatttcct tttgtcaggt caagcatctc aatgaaagac atctctgtca
      301 ggttaaactt gagtaattta cgcgattcat cggacagaat catcagactt tcattattaa
      361 aggccaggcc ggatatatcg ttaacacgta tatcatttag tgcctctgga atagttgctt
      421 gctttacact cagcaggtct ggagaaagtt gatatataaa tattttactt gggcgtcttt
      481 cttttgcgac aaacagcatc cgatcatctg ctttccatgc cagcccttca aaccctctat
      541 tctttttatt gaatttttct aactgaattt tatttcgaaa ggagatatgc atatcttttg
      601 taactgtaaa aaagaatagt tcagaagtct tttcgtctac tgcagcaaaa atgttccctt
      661 gtatgtgttc aattgtttca gcatcagaga taaaaggcag cggggctctg tccatgatgt
      721 cgccactttt ggttatttta agtagctgtg cagggctatt ctgggtggca tacaggaaat
      781 catcctcttt attataagta agagatgata agttcaccgt atttggtaat t
//
LOCUS       DAZFJL010000334          821 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFJL010000334 DAZFJL010000000
VERSION     DAZFJL010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.337"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(1..292)
                     /locus_tag="ACHJ8C_004819"
                     /pseudo
     CDS             complement(1..292)
                     /locus_tag="ACHJ8C_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819258.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
     gene            complement(289..819)
                     /locus_tag="ACHJ8C_004820"
     CDS             complement(289..819)
                     /locus_tag="ACHJ8C_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO5554498.1"
                     /translation="MSLRRMTSGELTLARSIFENTIDYNKVWFHNEKYLPGQGERTAM
                     TPNGEMYYPDAVYSSDFSSQSIPGERETVAGASHLFIHEMMHVWQYQKGYAVKLRGLF
                     SWAANYHYDLNLQSIVSYSMEQQASIVADYWLLINYGLVADLANVNYVGDTTESVNTL
                     SEKYKKVLNLFPAGVI"
BASE COUNT          266 a          176 c          124 g          255 t
ORIGIN      
        1 tagtattctt ttttacttcc gggagagagc atatattttc cgatataagt aacatctgaa
       61 gatagctcac ttatgtcaat acatacgggt tcgaactgac cagtcatttt ttcagtttta
      121 ctacattgcc atatttacct tcatataaaa tataatttaa aatcatactc ccatcgtctg
      181 gttttgaaca aacccgtccg tcttgtaaag atatattacc gggcaacaat gggcgaacat
      241 aatcaccaac agtgcatccc gacaaaaaga acaataaaaa aaatctcttc atatgacgcc
      301 cgcaggaaat aaattaagca cctttttgta tttttccgat agcgtgttca ctgattcagt
      361 tgtatcacct acataattaa catttgccaa atcagctacc agtccgtagt taatcaatag
      421 ccaataatca gctactatag atgcttgttg ctccatggaa taagaaacaa ttgactgtaa
      481 attaagatcg taatgataat ttgccgccca ggaaaataaa ccacgcaact taactgcata
      541 ccctttctga tattgccaaa catgcatcat ttcatgaata aacaagtgag aagcacctgc
      601 tactgtttca cgctcaccag gtattgattg cgatgagaaa tctgaactat aaacagcatc
      661 aggataatac atttccccgt ttggggtcat cgctgttctt tccccttgac cgggcaaata
      721 tttttcattg tggaaccata ccttgttata atcaattgta ttctcaaaaa tcgatctggc
      781 taatgttaat tcaccagatg tcattctcct taatgacata t
//
LOCUS       DAZFJL010000335          814 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZFJL010000335 DAZFJL010000000
VERSION     DAZFJL010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.338"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..174)
                     /locus_tag="ACHJ8C_004821"
     CDS             complement(<1..174)
                     /locus_tag="ACHJ8C_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO5554499.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHCARK"
     gene            complement(171..581)
                     /locus_tag="ACHJ8C_004822"
     CDS             complement(171..581)
                     /locus_tag="ACHJ8C_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO5554500.1"
                     /translation="MKQTIFLRTKQQQQAAINAILATPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMVADIARQVQWRGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(589..795)
                     /locus_tag="ACHJ8C_004823"
     CDS             complement(589..795)
                     /locus_tag="ACHJ8C_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554501.1"
                     /translation="MVELIFSALRILGAMWMVATFIVVASSFVRLVGEGKDLVGVLFG
                     SIFLWVIIGVMPVVVAKVAWRFVS"
BASE COUNT          190 a          221 c          174 g          229 t
ORIGIN      
        1 tttcctcgca caatgtctta gccaccggat atcccacagg tgagccgtgt agttgaaggt
       61 ttttacgtca gattcttttg ggattggctt gcgtttattt ctggagcgtt tcgttggaag
      121 gtatttgcag ttttcgcaga tgatgtcggt gaaacttcgt cgctgtcgtc tcattcgtac
      181 ctcctgtcgg taaatctgac accctgacca atagcccatg ctgtcgtgta ctcaatcaga
      241 cttgccatac gcttcacact catctgcgcg ctgctttcgc gaatgttgac gcattcgcct
      301 tcaagcccgg gtaaaacatc agcttcctgt ttcgttgcca ctgcatgacc gcttatcaac
      361 aaaaccttcc attgttctgg ttttaaccat ttgccgcgcc actgaacttg cctagcgata
      421 tctgcgacca tcgcgtgaaa ttttgcgttc tggtcaaggt tgcgcttgta gtcagtaatg
      481 cggatggtaa ctggcttgtc tttatcgagt ggtgttgcga ggatggcgtt gattgcggct
      541 tgctgttgtt gcttagttcg gaggaagatt gtttgcttca tcgttacctc aactcacaaa
      601 acgccacgcc acttttgcta cgacaacagg cataacaccg ataatcaccc acaggaaaat
      661 gctaccgaaa agcacaccaa ccaggtcttt accttcgcct accagccgga caaaactgct
      721 ggcaaccaca atgaacgtcg ccaccatcca catagcaccg agaatcctca atgcagaaaa
      781 aatcaactca accacgattt actctccccc aaat
//
LOCUS       DAZFJL010000336          809 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFJL010000336 DAZFJL010000000
VERSION     DAZFJL010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.339"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
BASE COUNT          275 a          165 c          148 g          221 t
ORIGIN      
        1 gcatttccat ttagttaagc gtttctcttt ataagataga tctcattgca taaacaaata
       61 aaacagaata aaaaccaagc tgaaccgttt cactatactc tacttaattc gagttacagg
      121 aaggcggcaa gcgagtgaat ccccaggagc ttacataagt aagtgattgg ggtaagcgaa
      181 cgcagtcgca gcacatgcaa cttgaagtat gacgagtata tatatctatt agctgtctcc
      241 cggacataag ctacagccag cactaacctg gaacgggtgc attataaaaa ggaacaaggg
      301 acaaatagct gaacaagtaa ccgcgttagt ctcttcccgc ataaccgtac cattcgtatt
      361 aagaagtatt gcccatatcc attcggcgaa agaggatctg ccatacatca agtccgtttt
      421 acttggcatc agatcatcgc cgtcattaac tctgttaaag tcggacaaac gattagcaag
      481 atctgtgatt caaaagtgca tagcgctaag gtgggactgg ttacagattc attaaccctg
      541 ttctttgtcg cacctatcga tcggtgtaat gccacaacag gtttatgtta atcagaacaa
      601 tgcgcccctc tataaagaga attctttgat aaggcaactt gaaaagggaa agctaactga
      661 tatcaatgta ccactttagt aatcatcaca ttatatacag gccaaaataa gtttcctgaa
      721 agccagtatc tctaaatcaa tcattaatgg tatattttat cgtcacaata aaaaattatc
      781 taaattctaa tacttatgta aataagtac
//
LOCUS       DAZFJL010000337          791 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFJL010000337 DAZFJL010000000
VERSION     DAZFJL010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.340"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(28..624)
                     /gene="fimE"
                     /locus_tag="ACHJ8C_004824"
     CDS             complement(28..624)
                     /gene="fimE"
                     /locus_tag="ACHJ8C_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO5554502.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLIHEKLKREEV"
BASE COUNT          243 a          191 c          149 g          208 t
ORIGIN      
        1 ttttaacttt attatcaata agttaaatca aacttcttct ctttttaatt tttcgtgtat
       61 gagattattt ctttcccata atccggcaaa acgagcagca ttactggcgg tataacgcac
      121 agtatggcga atatttcgat gcccgagata atcctgaatt aaacgggtat ctgcaccacg
      181 ctccgccagt tcataaccac aagcatgcct taacatatga ggatgagtct gcgttacggt
      241 tccagcttca ataccggcat cgcgaataat gcgataggcc tgctggcgag aaagccgact
      301 cccgcggcga gaaataaata tagcgtcggt ccggtcagcg cctttccagt tagcacgttc
      361 ctgggtccag cgttccacgg cttcacgctc atcaaaacgt aacgggtgaa cggtagaaaa
      421 tccgttcttc agtcggcgaa tatttattcg accttcatta aggtcaagat cctgataatg
      481 cagatcaagc agttcactaa tacgcatccc atgccgatat gccaacagaa taagacaata
      541 atctctggct cccgttgccc cgtaacacac cgcctgcatc atggcctgaa cttctttacc
      601 ggtaagataa cgacgtttac tcacaatagt agtactcctg actgagatat atttgaatga
      661 atacctatag gaaacctcaa tcggtcaaaa ttagcccaat agataaaaag ataaccacac
      721 acgaagtgat gtggctatca gtcaattaca ttaaatatca tacaaaaata aaatatcacc
      781 gatgtgacta c
//
LOCUS       DAZFJL010000338          786 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZFJL010000338 DAZFJL010000000
VERSION     DAZFJL010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.341"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            187..>786
                     /locus_tag="ACHJ8C_004825"
     CDS             187..>786
                     /locus_tag="ACHJ8C_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554503.1"
                     /translation="MSPDNIGSAVNRFSGWPSNDSLKTDKESTTSPVTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQLLIESALQTLHVMNLNNLETNRDEEGHYLAFE
                     YNVDTSDRITVSAYNEKKPGCYGQCLVIQDDETNLFNLIKIKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPVYFPLKTNEPVTQEDVLL"
BASE COUNT          253 a          157 c          149 g          227 t
ORIGIN      
        1 ttgtttcatt agtaataatc acttaaatgt atcgatgaaa caggtatgac ttaccgggag
       61 tgattgtttc tgagtgaaca gaggccactt tcgcctgata aatgaaaaat gagcgatttt
      121 tgcaagttag tttttctata gccgagtacg ctcagcgcct cgcttgatta caatgaggtt
      181 gctgctatga gtcctgataa tatcggttcg gctgtcaatc gcttctcagg ttggccatct
      241 aatgattctc taaaaacaga taaagaatca acgacatcac ccgtgacagt gcaggtaata
      301 aagatgattg ccgccatcct tgacccacat cggatagtcg ttggaatcga tacactttat
      361 acaacgcagc gcgaaacatt tacctcagag agtcaactgc tcattgaatc agcattgcaa
      421 acacttcatg tgatgaattt aaataacctg gaaacaaatc gcgatgaaga aggtcactat
      481 ctggcttttg aatataacgt tgacacttct gacagaataa ccgtttctgc ttataatgaa
      541 aaaaaacctg gctgctatgg ccaatgtctt gtaatacagg atgacgaaac aaacttattt
      601 aacctgataa aaattaaaat tttcgaagcc agacataatt atgtcgctca attgatgagc
      661 gatccggaat ttatgttcaa attcagttat agcgctcaac aaaatagatt agaacctcat
      721 cttctggttc ccgtttattt ccctctaaaa acaaacgaac cggttacaca agaggatgta
      781 ctgtta
//
LOCUS       DAZFJL010000339          778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFJL010000339 DAZFJL010000000
VERSION     DAZFJL010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.342"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..189
                     /gene="traL"
                     /locus_tag="ACHJ8C_004826"
     CDS             <1..189
                     /gene="traL"
                     /locus_tag="ACHJ8C_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFO5554504.1"
                     /translation="ITTSKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTAL
                     LRGIFHNVPDSCFRQWIK"
     gene            211..777
                     /gene="traE"
                     /locus_tag="ACHJ8C_004827"
     CDS             211..777
                     /gene="traE"
                     /locus_tag="ACHJ8C_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFO5554505.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
BASE COUNT          200 a          173 c          185 g          220 t
ORIGIN      
        1 atcacaacat cgaaatatct gttcggtatt ggtgcagcgg ttctggttta tttcgggatt
       61 aaaaaactga aaaaagggcg gggcagttcc tggttacgtg acctgattta ctggtatatg
      121 ccaacagccc tgctgcgcgg tatttttcat aatgttcccg attcgtgttt ccggcagtgg
      181 attaaataga actgatacca ggattgttat atggaacacg gtgcccgttt aagtaccagt
      241 cgtgtaatgg ccatcgcctt tatatttatg tcagtgctta ttgttctcag cctctctgtt
      301 aacgtcattc agggggtgaa taactaccgt cttcagaatg agcaacgcac tgccgtgacg
      361 ccaatggcat ttaatgcccc ctttgccgtg tcgcagaaca gtgccgacgc ctcttattta
      421 cagcagatgg cgctgtcatt tattgccctc cgtctgaatg tttcatcaga aaccgtcgat
      481 gcctcacatc aggcgcttct gcaatatatc cgcccgggcg cacagaacca gatgaaagtt
      541 attctggctg aagaagcgaa gcgtattaaa aacgataacg tgaactcagc ctttttccag
      601 accagtgttc gtgtctggcc tcagtatggc cgtgtggaaa ttcgtggtgt gcttaaaacc
      661 tggattggtg attcaaaacc tttcactgat atcaaacatt acatccttat tctgaagcgg
      721 gaaaacgggg tgacctggct ggataatttc ggggaaacag acgatgagaa aaaataat
//
LOCUS       DAZFJL010000340          769 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZFJL010000340 DAZFJL010000000
VERSION     DAZFJL010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.343"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..644
                     /gene="wzx"
                     /locus_tag="ACHJ8C_004828"
     CDS             <1..644
                     /gene="wzx"
                     /locus_tag="ACHJ8C_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_158227893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O132 family O-antigen flippase"
                     /protein_id="HFO5554506.1"
                     /translation="QEFKDGWHLFISTAAISMYTTSIVVVLGIFSGPIAVGYFTAADR
                     IRQAVQGLITPITQACYPRINYLYRTSPEKGFELVRKLLYFQGGITLTLSIILMMFAS
                     VIVKILFGNGFERSDSVLTIMAFCPFVVGISNVLGIQILVALGYKKVFSKILLCSGGL
                     CLILIGPSTYLYSENGAALSVLITEVFVTIAMLIVVLAKKVPIFKRENLNNEV"
     gene            634..>769
                     /locus_tag="ACHJ8C_004829"
     CDS             634..>769
                     /locus_tag="ACHJ8C_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001767431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5554507.1"
                     /translation="MKYDYIIVGAGLYGAVLACELHKAGKKVMVIERREHIGGNIYTE
                     N"
BASE COUNT          229 a          108 c          168 g          264 t
ORIGIN      
        1 ttcaggaatt taaagatgga tggcatttgt ttatttcaac tgcggcaata agcatgtata
       61 ccacaagtat tgttgtagtt ttaggaattt tttctggacc aattgccgtc ggttatttta
      121 ctgctgcaga tcgtattagg caagcagttc aggggttaat tacccctatt acgcaggcat
      181 gctatccaag gattaattat ctttacagaa cttctccaga aaagggcttt gagctagtca
      241 gaaaattatt atattttcag ggaggtataa ccttaacgtt aagtataata ctcatgatgt
      301 tcgcaagtgt tatcgttaaa atcttatttg gaaacggttt cgaaagatca gattctgtac
      361 taaccattat ggcattttgc ccattcgtag tagggataag taatgtgtta ggaattcaga
      421 tattagttgc attaggatat aaaaaagtat tcagtaaaat acttttatgc tcaggcgggt
      481 tatgtttgat attgattggc ccgtctacat atctctatag tgagaatggt gcagcattaa
      541 gtgtgctaat aactgaggtt tttgtgacga ttgcaatgct tattgttgtg cttgcaaaaa
      601 aagtccctat ttttaaaaga gagaatttga ataatgaagt atgattacat tattgttgga
      661 gctggtctct acggcgctgt cctggcctgt gaacttcata aggctggaaa aaaagttatg
      721 gttattgaga gacgtgagca tattggtggt aatatttaca ctgaaaatg
//
LOCUS       DAZFJL010000341          764 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFJL010000341 DAZFJL010000000
VERSION     DAZFJL010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.344"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>764
                     /locus_tag="ACHJ8C_004830"
     CDS             <1..>764
                     /locus_tag="ACHJ8C_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO5554508.1"
                     /translation="ADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLGRQVGVP
                     YIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEGDAEWEA
                     KILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEI
                     VGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHT
                     KFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTG"
BASE COUNT          177 a          186 c          218 g          183 t
ORIGIN      
        1 gccgactatg ttaaaaacat gatcaccggt gctgctcaga tggacggcgc gatcctggta
       61 gttgctgcga ctgacggccc gatgccgcag actcgtgagc acatcctgct gggtcgtcag
      121 gtaggcgttc cgtacatcat cgtgttcctg aacaaatgcg acatggttga tgacgaagag
      181 ctgctggaac tggttgaaat ggaagttcgt gaacttctgt ctcagtacga cttcccgggc
      241 gacgacactc cgatcgttcg tggttctgct ctgaaagcgc tggaaggcga cgcagagtgg
      301 gaagcgaaaa tcctggaact ggctggcttc ctggattctt acattccgga accagagcgt
      361 gcgattgaca agccgttcct gctgccgatc gaagacgtat tctccatctc cggtcgtggt
      421 accgttgtta ccggtcgtgt agaacgcggt atcatcaaag ttggtgaaga agttgaaatc
      481 gttggtatca aagagactca gaagtctacc tgtactggcg ttgaaatgtt ccgcaaactg
      541 ctggacgaag gccgtgctgg tgagaacgta ggtgttctgc tgcgtggtat caaacgtgaa
      601 gaaatcgaac gtggtcaggt actggctaag ccgggcacca tcaagccgca caccaagttc
      661 gaatctgaag tgtacattct gtccaaagat gaaggcggtc gtcatactcc gttcttcaaa
      721 ggctaccgtc cgcagttcta cttccgtact actgacgtga ctgg
//
LOCUS       DAZFJL010000342          761 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZFJL010000342 DAZFJL010000000
VERSION     DAZFJL010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.345"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..605
                     /gene="ydeO"
                     /locus_tag="ACHJ8C_004831"
     CDS             <1..605
                     /gene="ydeO"
                     /locus_tag="ACHJ8C_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO5554509.1"
                     /translation="NENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLN
                     SEERNHHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKD
                     ICDCLYISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTS
                     YFIYAFRKHFGNSPKRVSKEYRCQRHTGMNTDNKMSALAI"
BASE COUNT          238 a          149 c          150 g          224 t
ORIGIN      
        1 ttaatgaaaa taccattaaa aaatatctac aatgcactaa catccagaca gtgacggtgc
       61 ctgttcctgc gaagttttta cgtgcttcaa atgtaccgac tggattgctt aatgaaatga
      121 ttgcttatct caactcggaa gaacgcaatc atcataattt ttcagaactt ttgctttttt
      181 cttgcctgtc tatttttgcc gcatgcaaag gtttcattac actattaact aacggtgtgc
      241 tatccgtttc cgggaaagtg agaaatattg tcaacatgaa gctggcgcac ccatggaagc
      301 tgaaagatat ttgtgactgc ctgtacatca gtgaaagcct gttgaagaaa aaactaaagc
      361 aagagcaaac gacattctca cagattcttt tagatgccag aatgcagcac gcaaaaaatt
      421 tgatacgcgt agaaggttca gtcaataaaa ttgccgaaca atgtggttat gcgagtacat
      481 cttattttat ttatgcgttc cgcaaacatt tcggcaacag tccaaagaga gtttctaagg
      541 agtaccgttg tcaaaggcac acgggtatga acacggataa caagatgagt gctttagcta
      601 tttgattatt tgctaacgag tagtcaacca cacacgctgc gtaagaatta aatggggcag
      661 ccattccctg ccccgcgttg tttttaggtg atatatttct tgaaataaat aagtgacagc
      721 catcacatat ttatgcattt gcataacccg ttgcatgatt a
//
LOCUS       DAZFJL010000343          753 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZFJL010000343 DAZFJL010000000
VERSION     DAZFJL010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.346"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(555..>753)
                     /locus_tag="ACHJ8C_004832"
     CDS             complement(555..>753)
                     /locus_tag="ACHJ8C_004832"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554510.1"
                     /translation="VDIIRHSNFEASEDDNAIEYHYAACLRLYRLLGDEAEDILVEEL
                     LQHPTITILRAIGFLVGHEIK"
BASE COUNT          239 a          143 c          112 g          259 t
ORIGIN      
        1 ctcctaagcc cttgaagtaa tctaaattgt ctaaatgctt atctgttaaa cctcgctgcg
       61 tagttcattt taaatgttta aaaaaatctc ttgcaaccgg tttactgtta aaaaggtaat
      121 tagatagtcg tgatgataca ccatatgaga acattatata atgttctgca cttctgtttg
      181 ctgaacttag atgataagtt ttcatatctg gttctcttta accttgggag aaggttacga
      241 cagtcgctct ctataaggac tgaagcgagg tggtcatttc ttatattccg tttaaccttg
      301 acgataaaac ggacatatca catcaacctt agccagatga ctccctccga taaaaacgga
      361 tagtctgcct gatcatctcc aaattgaata ctcggcataa ttatctaata aatatcgtaa
      421 atttatagaa attgatttat tgcattaact cgcgcaacat cttcaattaa tacgtctttt
      481 gcgacaatag ataattgctc ggaatatgct tcagagattt cattaagtat acaaaatgta
      541 tttttattaa tggttcattt tatctcatgt ccaactaaaa aaccgattgc ccttaatatg
      601 gttattgttg gatgttgtaa taattcctca accaatatat cctctgcttc atcaccaagt
      661 aatctataaa gcctcaaaca tgcagcataa tggtactcta ttgcattatc atcttctgaa
      721 gcttcaaaat tagaatgccg tattatatca act
//
LOCUS       DAZFJL010000344          746 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFJL010000344 DAZFJL010000000
VERSION     DAZFJL010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.347"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..97
                     /gene="ymgI"
                     /locus_tag="ACHJ8C_004833"
     CDS             <1..97
                     /gene="ymgI"
                     /locus_tag="ACHJ8C_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFO5554511.1"
                     /translation="NYLNNSERSLVKQILIEDEIIVCATYLIPDN"
     gene            99..443
                     /gene="ymgG"
                     /locus_tag="ACHJ8C_004834"
     CDS             99..443
                     /gene="ymgG"
                     /locus_tag="ACHJ8C_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO5554512.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            447..>746
                     /locus_tag="ACHJ8C_004835"
     CDS             447..>746
                     /locus_tag="ACHJ8C_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO5554513.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITA"
BASE COUNT          213 a          142 c          207 g          184 t
ORIGIN      
        1 gaattatctg aataatagcg agcgttcttt agttaagcag atcttaattg aagacgaaat
       61 tattgtgtgt gcaacttact taataccgga caactaagtt gaagaaaaaa atactcgcct
      121 tcgggcttat ctcagcattg ttctgctcca cgccagcaat ggcggatatg aatcgcacca
      181 cgaaaggtgc attgctaggc gcaggcgttg gtttacttac tggcaatggt gttaatggcg
      241 tactgaaagg tgcagctgtt ggcgctggtg ttggtgcagt aacagaaaaa ggccgcgacg
      301 gtaaaaatgc gcgtaaaggt gcaaaagtgg gtgccgccgt tggtgctgtc actggcgttc
      361 tgaccggcaa tggcctcgaa ggggcgatta aaggtgccgt gattggcggt acgggtggtg
      421 caattctggg taaaatgaaa taaggaatga tgatgaaaaa atttgcttta ctggcgggtc
      481 tgtttgtttt cgcaccaatg acctgggcgc aggactacaa tattaagaac ggtttgccat
      541 cagaaaccta tattacttgc gcagaagcca acgaaatggc aaaaactgac agcgcacagg
      601 ttgcagaaat tgttgcggta atgggtaatg ccagcgttgc cagccgtgat ttaaaaattg
      661 agcaatctcc ggaactgagc gccaaagtgg tagaaaaact gaatcaggtt tgcgctaaag
      721 atccgcaaat gctgttgatc acggca
//
LOCUS       DAZFJL010000345          745 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZFJL010000345 DAZFJL010000000
VERSION     DAZFJL010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.348"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            31..>745
                     /locus_tag="ACHJ8C_004836"
     CDS             31..>745
                     /locus_tag="ACHJ8C_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5554514.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          247 a          129 c          164 g          205 t
ORIGIN      
        1 tgtaaataaa ttttcgagga gatcattcca gtgggacgta aatgggccaa tattgttgct
       61 aaaaaaacgg ctaaagacgg tgcaacgtct aaaatttatg caaaattcgg tgtagaaatc
      121 tatgctgctg ccaaacaagg tgaacccgat ccagaattaa acacatcttt aaaattcgtt
      181 attgaacgtg caaagcaggc acaagttcca aagcacgtta ttgataaagc aattgataaa
      241 gccaaaggcg gcggagatga aacgttcgta cagggacgtt atgaaggctt tggtcctaat
      301 ggctcaatga ttattgctga gacattgact tcaaatgtta accgtacgat tgctaacgtt
      361 cgcacaattt tcaataaaaa aggcggcaat atcggagcgg caggttctgt cagctatatg
      421 tttgacaata cgggtgtgat tgtatttaaa ggaacagacc ctgaccatat ttttgaaatt
      481 ttacttgaag ctgaagttga tgttcgtgat gtgactgaag aagaaggtaa cattgttatt
      541 tatactgaac ctactgacct tcataaagga atcgcggcgc taaaagcagc tggaatcact
      601 gagttctcaa caacagaatt agaaatgatt gctcaatctg aagttgagct ttccccagaa
      661 gatttagaaa tctttgaagg gcttgttgat gcccttgaag atgacgacga tgtacaaaaa
      721 gtttatcata acgtcgcaaa tctct
//
LOCUS       DAZFJL010000346          738 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFJL010000346 DAZFJL010000000
VERSION     DAZFJL010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.349"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..480
                     /locus_tag="ACHJ8C_004837"
     CDS             <1..480
                     /locus_tag="ACHJ8C_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO5554515.1"
                     /translation="GQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAA
                     YLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAF
                     TLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
BASE COUNT          185 a          181 c          167 g          205 t
ORIGIN      
        1 ggtcagattg gtactttcgc catcaacttc tcccgcccgg cgggtcaggt gattgcacag
       61 tattatgaat tcctgcgcct cggtcgtgaa ggctatacca aagtacagaa cgcttcctac
      121 caggttgctg cttatctggc ggatgaaatc gccaaactgg ggccgtatga gttcatctgt
      181 accggtcgcc cggacgaagg catcccggcg gtttgcttca aactgaaaga tggtgaagat
      241 ccaggataca ccctgtacga cctctctgaa cgtctgcgtc tgcgcggctg gcaggttccg
      301 gctttcactc tcggcggtga agccaccgac atcgtggtga tgcgcattat gtgccgtcgc
      361 ggcttcgaaa tggactttgc tgaactgttg ctggaagact acaaagcctc cctgaaatat
      421 ctcagcgacc acccgaaact gcagggtatt gcccagcaga acagctttaa acacacctga
      481 taacatcacg ttgtaaaaac cgaatgccca acctttaaaa aacaggtggg gtattcggct
      541 atttattaat tgcaataata tattacctgt ctattcatcg tgttgataag atatatgtta
      601 tatgaatgtt atggggcata gcgatactgc ttaatattac gatgatgaat tatttgctta
      661 ctattaattc ctctgcgttc ttaagtcccc tttttatata ataaatccag tcatccagcc
      721 cgagtatcat cgctcatt
//
LOCUS       DAZFJL010000347          727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZFJL010000347 DAZFJL010000000
VERSION     DAZFJL010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.350"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            80..>727
                     /locus_tag="ACHJ8C_004838"
     CDS             80..>727
                     /locus_tag="ACHJ8C_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO5554516.1"
                     /translation="MKNLKSTENRITKLLQEKGWSQAELARKLGVSAQSVQYWTTGKT
                     FPRGNRLAQLSEISGYPQSWFLGEITSRQFSSKEKHQARTDSVVFNVLDVEFSCGDGT
                     HVRGDFIDVVRSIELDPEYARRLVGNRAFQNIEIGNARGDSMAPTIAPGDLLFLDKTV
                     NYFDGDGIYAFCFDGECYVKRLQKIGSKIMVLSDNPNYQSWSIEKDALELLYIQSK"
BASE COUNT          244 a          139 c          163 g          181 t
ORIGIN      
        1 catgcaagtc cacaaaagat cgaatacact aaaatcaagt aaaaatttat cgaaaccaag
       61 taaccagcaa ggattaacaa tgaaaaactt aaagagcact gaaaatcgaa ttacaaagct
      121 gctacaggag aaaggctgga gccaagcaga gctggcccga aaattaggtg tcagcgcaca
      181 gtccgtacag tactggacaa caggaaaaac atttccaagg ggaaaccggc ttgcccagtt
      241 atccgaaata agtggttatc cgcaatcgtg gtttttagga gaaatcacgt cacggcagtt
      301 ctcttcaaag gaaaaacatc aagcaagaac tgacagtgtg gtctttaacg tgctcgatgt
      361 tgagtttagt tgcggcgatg gaacacatgt ccgtggggac tttatcgatg tagttcgctc
      421 aatcgagctt gatcctgaat atgcccgtcg ccttgttggc aatagagcat ttcaaaacat
      481 agagattggt aacgcaagag gagatagtat ggctccaaca atagccccag gagatcttct
      541 ttttctcgat aaaacagtga attactttga tggtgatggc atttatgctt tttgctttga
      601 tggtgaatgc tacgtcaaaa gattacaaaa gataggaagt aaaatcatgg tgttatctga
      661 taacccaaat taccaatcct ggagcattga aaaagatgct cttgagctgc tttatatcca
      721 atcgaag
//
LOCUS       DAZFJL010000348          714 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZFJL010000348 DAZFJL010000000
VERSION     DAZFJL010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.351"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..372
                     /locus_tag="ACHJ8C_004839"
     CDS             <1..372
                     /locus_tag="ACHJ8C_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO5554517.1"
                     /translation="TLRKAHRTGAKFMMNNNSLFAIRLLKDSEGNYLWRPGLELGQPS
                     SLAGYGIAENEQMPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTT
                     KRTGGMLVDSQAIKLLKIAAA"
     gene            423..611
                     /locus_tag="ACHJ8C_004840"
     CDS             423..611
                     /locus_tag="ACHJ8C_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554518.1"
                     /translation="MILKQDLKWSPDGMRVEIIRAGEYEDKELPERVREIATAAGIVS
                     DKRTPVARGADKSKKQHS"
BASE COUNT          180 a          171 c          201 g          162 t
ORIGIN      
        1 acgctgcgta aggcacaccg caccggcgcg aagttcatga tgaacaacaa cagcctgttt
       61 gccatccgtc tgctgaaaga cagcgagggt aactatctgt ggcgtccggg gctggagctg
      121 gggcagccgt cctctctggc gggttacggt atcgctgaaa acgaacagat gccggatatc
      181 gccgctgatg cgaaagccat tgcatttggt aacttcaaac ggggttacac catcgttgac
      241 cgtatcggta cccgcattct gcgtgacccg tacaccaata aaccgtttgt cggtttttat
      301 accaccaagc gcaccggcgg catgctggtc gattcgcagg ccatcaaact gctgaagatt
      361 gcagcggcgt aatcattcag ggggcgcaga agtgcgcccc tgttctgaca ggtgaaagaa
      421 tcatgatcct gaaacaagat ctgaaatggt caccggacgg tatgcgtgtt gagattattc
      481 gggccggtga gtatgaagat aaagaattac ccgaacgggt acgcgaaatt gccactgcag
      541 ctgggattgt ctctgataag agaacacctg ttgcgcgggg ggctgataag tctaaaaaac
      601 agcattcata gaggttgccc aaatgatgcc cactctggaa gagcttcgtg ttcagtgccg
      661 gattgatgat gacaatgaac aggagaattc tcttcttatg atgtatctgg ctgc
//
LOCUS       DAZFJL010000349          695 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZFJL010000349 DAZFJL010000000
VERSION     DAZFJL010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.352"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>695
                     /locus_tag="ACHJ8C_004841"
     rRNA            <1..>695
                     /locus_tag="ACHJ8C_004841"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          176 a          157 c          229 g          133 t
ORIGIN      
        1 ctagctggtc tgagaggatg accagccaca ctggaactga gacacggtcc agactcctac
       61 gggaggcagc agtggggaat attgcacaat gggcgcaagc ctgatgcagc catgccgcgt
      121 gtatgaagaa ggccttcggg ttgtaaagta ctttcagcgg ggaggaaggg agtaaagtta
      181 atacctttgc tcattgacgt tacccgcaga agaagcaccg gctaactccg tgccagcagc
      241 cgcggtaata cggagggtgc aagcgttaat cggaattact gggcgtaaag cgcacgcagg
      301 cggtttgtta agtcagatgt gaaatccccg ggctcaacct gggaactgca tctgatactg
      361 gcaagcttga gtctcgtaga ggggggtaga attccaggtg tagcggtgaa atgcgtagag
      421 atctggagga ataccggtgg cgaaggcggc cccctggacg aagactgacg ctcaggtgcg
      481 aaagcgtggg gagcaaacag gattagatac cctggtagtc cacgccgtaa acgatgtcga
      541 cttggaggtt gtgcccttga ggcgtggctt ccggagctaa cgcgttaagt cgaccgcctg
      601 gggagtacgg ccgcaaggtt aaaactcaaa tgaattgacg ggggcccgca caagcggtgg
      661 agcatgtggt ttaattcgat gcaacgcgaa gaacc
//
LOCUS       DAZFJL010000350          687 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZFJL010000350 DAZFJL010000000
VERSION     DAZFJL010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.353"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>687
                     /locus_tag="ACHJ8C_004842"
     CDS             <1..>687
                     /locus_tag="ACHJ8C_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc47 family transposase"
                     /protein_id="HFO5554519.1"
                     /translation="ERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEH
                     LPLYRQSEIFARQGVELSRALLSNWVDACCQLMTPLNDALYRYVMNTRKLHTDDTPVK
                     VLAPGLKKTKTGRIWTYVRDDRNAGSSSPPAVWFAYSPNRQGKHPEQHLRPFRGILQA
                     DAFTGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIE
                     DEIRGLPESER"
BASE COUNT          174 a          183 c          200 g          130 t
ORIGIN      
        1 gaacgggtaa aaaaagcctg tacaaaatgt gactgcatcg ttgaagcacc ggcgccgtcc
       61 cgcccgatag agcgtggtat cgcgggcccc ggattacttg cccgcgtgtt aacgggaaaa
      121 tactgcgaac acctgccact gtatcgtcag agtgaaatct ttgcccgaca gggtgtcgaa
      181 ctgagccgtg cattactctc caactgggtt gacgcgtgct gccagttaat gacaccgctg
      241 aatgatgccc tgtaccgtta tgtgatgaat acccgcaagc ttcacactga cgacacaccg
      301 gtaaaagtac tggcaccggg cctgaaaaag acgaaaacag ggcgcatctg gacgtatgtc
      361 cgggatgatc gcaatgcggg ttcgtcatct cctccggcgg tctggttcgc gtactcaccg
      421 aaccggcagg ggaaacaccc ggagcaacac ctccgcccct tccggggtat cctgcaggcg
      481 gatgcgttca caggttatga caggctgttc agtgcagaac gtgaaggtgg tgcgctgaca
      541 gaagttgcgt gctgggccca tgcccggaga aaaatccacg atgtatacat cagcagcaaa
      601 agtgcgacgg cagaagaagc cctgaagcga atcagtgaac tgtacgccat cgaggatgaa
      661 atacggggat taccggagtc agagcgt
//
LOCUS       DAZFJL010000351          683 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFJL010000351 DAZFJL010000000
VERSION     DAZFJL010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.354"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(240..506)
                     /locus_tag="ACHJ8C_004843"
                     /pseudo
     CDS             complement(240..506)
                     /locus_tag="ACHJ8C_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          206 a          140 c          151 g          186 t
ORIGIN      
        1 gctgcacttg ttgtagagag tatttgtaaa aacattttat gtcgctaaat aacatgacga
       61 tttcattatg catgttattt ataaacagcg actaaacaga atattattgc gaacaaggga
      121 agtgtcagga aaggtcaaga ggtactaata gtttatatgg ctataaacat tagctatggt
      181 taatgtttgc ctggtctaac taacacggaa ttgctctaaa cagataagtg cagggtttat
      241 taaagctgac cgacagactt tcttaacctg gtaatatctc tcttaggtga ctctccaaac
      301 atccgcgaat attcccgact gaaatgggat aagctttcgt aaccgacagc ataggctgca
      361 gtggtgacat cgtaatgttc attcagcatc agtcgcctgg cttcattgag gcctagccat
      421 ttccgatacc ggagtgaact cattcccgca agctgtctga agtgttgatg gaaagagggg
      481 gtgttcattt gcacgcggtt tgccagcctg actcttcaac gctgagaaga tgcggcaaac
      541 ggcaaacctg cataccagcc agcctatatc cggtatgcac aggccaatta tgaaagagga
      601 gaggctttat gttctggaac cctgaagtaa taacgacgac cggaaaatcg cttcgcatca
      661 ctcaaattca atttaacata ata
//
LOCUS       DAZFJL010000352          672 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZFJL010000352 DAZFJL010000000
VERSION     DAZFJL010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.355"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..>672)
                     /locus_tag="ACHJ8C_004844"
     CDS             complement(<1..>672)
                     /locus_tag="ACHJ8C_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554520.1"
                     /translation="IENEDVLNTSLLRDVTITEIHGQFSRILSGLPALSNFDFNFNRT
                     GVEGYSDLKIPFKVIMNAIPSTNIHAFIGRNGCGKTTILNEMIEAITSPNREDVFFTK
                     KGRFSEEKIPNGYFRSLISVSFSAFDPFSPPEEQPDPSKGTQYFYIGLKDVNNKNLKS
                     LRDLQIEFVTSLIGCLRVEWKKKIWLSAIDNLSSDRNFADMGLSILQTRYTNLRNSHA
                     HHQVD"
BASE COUNT          189 a          122 c          130 g          231 t
ORIGIN      
        1 ttatctacct gatgatgagc atgagaattt ctaagattag tatatcttgt ttgtaggatg
       61 cttaatccca tatccgcgaa gtttctatca ctacttagat tatctattgc gctaagccat
      121 attttctttt tccattccac tcttaaacaa cctataaggg aggttacaaa ttcaatttga
      181 agatcacgaa gagatttgag gtttttatta tttacatctt tcaatccgat gtagaaatac
      241 tgagtccctt tcgatgggtc aggctgttcc tcgggggggg agaatggatc aaatgcgcta
      301 aacgacacag atattagtga tctgaagtac ccatttggaa ttttttcttc actgaatcta
      361 ccctttttgg taaaaaatac atcttcgcga ttggggctgg ttattgcttc gatcatttca
      421 ttaagaatgg ttgtcttccc acaaccattt ctgccaatga atgcatggat atttgtgctt
      481 ggaattgcat tcattattac cttaaatggt atttttaaat cgctatatcc ctctacacca
      541 gttctgttga agttaaagtc aaagttagaa agcgcgggga gaccggacaa tatacgagaa
      601 aactgcccgt gtatttctgt aatagtaaca tctcggagta aggatgtgtt gagaacgtct
      661 tcattttcaa tg
//
LOCUS       DAZFJL010000353          670 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFJL010000353 DAZFJL010000000
VERSION     DAZFJL010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.356"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            24..614
                     /locus_tag="ACHJ8C_004845"
     CDS             24..614
                     /locus_tag="ACHJ8C_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bfpT-regulated chaperone"
                     /protein_id="HFO5554521.1"
                     /translation="MLMNATGTSFSSFGISSYRENSFWNCFRGKNDEVVKCSMGKRTI
                     RFSVHKFSSNILKMVGKQCTKNIDGWIKDERVVYPSRVINQEIDNYCFQKNVKISTEE
                     RKRVFSLVSQEYELALDVKAAQSSINHTIMGSASFCKKMDALSGGMSRNVKNRTSDSI
                     ANLLADKFYQKHIDSDVDIVKLRNEIPDYLRRAIQA"
BASE COUNT          223 a           91 c          161 g          195 t
ORIGIN      
        1 aatttttata aaaaggaatt gctttgttga tgaatgctac aggtacgtct tttagctctt
       61 ttgggataag tagctataga gaaaactcct tttggaactg tttccgaggg aagaatgatg
      121 aggtcgtaaa atgctcaatg gggaagcgga cgattcgctt ttctgttcac aaatttagta
      181 gcaacatact gaaaatggtg ggcaagcagt gtactaaaaa tattgatgga tggataaaag
      241 atgagcgggt agtatatccc tcaagggtga tcaaccaaga gattgataat tactgctttc
      301 aaaaaaatgt aaaaatttcc actgaagaga ggaaaagagt cttttctctt gtgagtcaag
      361 aatatgaact ggctcttgat gttaaggcag cacaaagctc tattaatcat accataatgg
      421 gaagtgcttc tttttgcaag aaaatggatg ctcttagcgg tggtatgagc agaaatgtaa
      481 agaatcggac atcagatagt atagcaaact tgctggcgga caagttttac cagaaacata
      541 ttgattcgga tgttgatatt gtgaaactac gaaatgagat tcctgattat ctgaggcgtg
      601 ctatacaggc atagcatagg ttcattgcag tggtaatatt ttgtgaagat tgcgtgatag
      661 gaagaaagaa
//
LOCUS       DAZFJL010000354          669 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFJL010000354 DAZFJL010000000
VERSION     DAZFJL010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.357"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..599)
                     /locus_tag="ACHJ8C_004846"
     CDS             complement(<1..599)
                     /locus_tag="ACHJ8C_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HFO5554522.1"
                     /translation="MRIISLRKEYLSLLPSMIASLFSANGAAAVIDSCQGYDIKASCQ
                     ASRQSLSGNIQDWSIADGQWLIFSGMVNNASGGAVFLQHSAEFTISPQNETGMTLFAD
                     NSVSGEYNNGGAIFAKENSTINLANVIFDSNVAGGYGGAIYSAGTNDTGAADLRVTNA
                     VFHNNIANDGKGGAIYTINNDVYLSDDAFNNNQAYTSTS"
BASE COUNT          190 a          150 c          130 g          199 t
ORIGIN      
        1 taacttgttg atgtatatgc ctggttatta ttaaaagcat catcacttaa atagacgtca
       61 ttattgatgg tataaattgc accgcctttg ccgtcattag cgatgttatt gtgaaatacg
      121 gcgttagtga cacgtaaatc ggcggcaccg gtatcattgg taccggcaga atagattgct
      181 ccgccatagc ctcctgcaac attactgtcg aaaataacat tcgcaagatt tatcgttgag
      241 ttttctttag caaagatagc cccaccatta ttatattcgc cactaaccga gttatcagca
      301 aacagggtca tccctgtttc attttgtggt gatatcgtaa attcagcact gtgctgcaaa
      361 aatactgcgc caccgctggc attattgacc atacccgaaa aaatcaacca ttgtccatcc
      421 gcgatgctcc agtcctgtat attacccgaa aggctttgcc tgctggcctg acagctcgct
      481 ttgatatcat atccctggca tgaatcaatg accgctgcag caccgttagc agagaaaaga
      541 gatgcaatca ttgacgggag taaagataaa tactccttgc gtagagagat aatccgcata
      601 ttcatctaac catcattttc tataagatgg cgtatttaat cgcattatgc gatatagggt
      661 atttttctt
//
LOCUS       DAZFJL010000355          666 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFJL010000355 DAZFJL010000000
VERSION     DAZFJL010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.358"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(208..>666)
                     /locus_tag="ACHJ8C_004847"
     CDS             complement(208..>666)
                     /locus_tag="ACHJ8C_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003033750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HFO5554523.1"
                     /translation="TDNRFKVVSAPQLRMDSGKEATFSVGEQVPVLGSVSYEDGKAVQ
                     SVTYRDSGVIFKVKPVITSSRISLNVNQQLSNFVKTDTGVNDSPTLLKREVDTSLTLK
                     DGDIVLLGGLAENKDSQASTGLSFLPKSWSQNSDEKSRTDMVILLQVKKV"
BASE COUNT          191 a          170 c          136 g          169 t
ORIGIN      
        1 gaaaaaaaca tcaaataaaa cagcaaaaaa atagaagttt tttgtgtaac tgtggctaga
       61 cttaaaggag aacagatatg tgataaaact atagacaaga gcgcgacatt gtacggtata
      121 gcaatagctt tttacataac tcatatcaaa ttattcgcac cgtcatttca tatgatggca
      181 gtgcgaataa tcagcctcaa acggaactca gacttttttc acctgcaaca ggatcaccat
      241 atccgtgcgg ctcttctcgt cagaattctg gctccagctt tttggcagaa atgataatcc
      301 tgtgctggcc tgcgaatcct tgttttctgc caggccaccc agcaacacga tatcgccatc
      361 tttcagggtc agcgacgtat ccacctcacg cttgagcaac gttggcgaat cattcacgcc
      421 cgtatccgtt ttgacaaagt tacttaactg ctgattgacg ttcagactga tgcgtgacga
      481 tgtgataacc ggcttcactt tgaatatcac ccccgaatca cgatacgtca ccgactgaac
      541 ggctttacca tcctcatagg acacgctgcc aagaaccgga acctgttcac caacggagaa
      601 cgtcgcctcc ttgcctgaat ccatgcgtaa ctggggggcg gataccacct taaagcggtt
      661 atccgt
//
LOCUS       DAZFJL010000356          649 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFJL010000356 DAZFJL010000000
VERSION     DAZFJL010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.359"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..254)
                     /locus_tag="ACHJ8C_004848"
     CDS             complement(<1..254)
                     /locus_tag="ACHJ8C_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554524.1"
                     /translation="MLASLGGLVSYAAKVHFKEAGNAMNELIANHDFDFRQLVTAAEG
                     QPVTDTFQIARAFGKRHQHVIRAIKCLRCSEEFSTTHFWAV"
     gene            complement(404..>649)
                     /locus_tag="ACHJ8C_004849"
     CDS             complement(404..>649)
                     /locus_tag="ACHJ8C_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO5554525.1"
                     /translation="LADANATIESLRADVSAGRKRLQVSATCAKSTTGASSMGDGESP
                     GLTADAELNYYRLRGGIDKITAQVNYLQEYIRTQCLK"
BASE COUNT          159 a          162 c          155 g          173 t
ORIGIN      
        1 acggcccaaa aatgggttgt cgagaattcc tcagaacatc tcaaacattt aatagcccta
       61 atcacatgct gatggcgttt accaaatgcc ctggcaatct ggaaggtgtc agttaccggt
      121 tgaccttctg ctgcggtaac taactggcga aagtcgaagt catgattcgc aattaattca
      181 ttcatggcgt tgcctgcttc tttgaaatga acctttgccg cataggaaac cagcccaccg
      241 aggctcgcca gcactaactg gtatcctcaa aggcccattc caaagggtca ggttcggtgt
      301 ttattgtgcg ctgcggtgcg cggtgaaata ccggtacaaa aatgccccgc atctgcgagg
      361 cattttcctg aaagtcactt gttaaatttc agtgaaatta aaattatttt aagcactgcg
      421 tcctgatgta ttcctgcagg tagttaacct gcgcggttat cttgtcgatt ccacctcgga
      481 gacggtaata attgagttca gcatctgctg taagtcctgg gctttctcca tcgcccatgc
      541 tgctggctcc ggtcgttgac tttgcacagg tggcggagac ttgcaggcgc ttacgcccag
      601 cagaaacatc agcacggaga ctttcgatag tcgcattagc atcagcaag
//
LOCUS       DAZFJL010000357          643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFJL010000357 DAZFJL010000000
VERSION     DAZFJL010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.360"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            84..>643
                     /gene="escD"
                     /locus_tag="ACHJ8C_004850"
     CDS             84..>643
                     /gene="escD"
                     /locus_tag="ACHJ8C_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFO5554526.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTLGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADKPLPVGKIIDIAGCCFIIGDIDHSLP
                     LSDVPERFSAKNQRKKRLILASVIGATFALSGAIGSYVLLSPKAEPPTFTRADVYQQL
                     KENKLHAITLVWHGKNIALYGRCESTT"
BASE COUNT          183 a          137 c          126 g          197 t
ORIGIN      
        1 tcctttctac tctattctta gacgatgtaa gttcaccata ttttttctca ttgttcaacc
       61 accaggatga ataaaattta aaaatgttat cctcatataa aataaaactt ctcaatggtg
      121 cgatgcgtaa tcgggaatta caacttccga tgggaaacct aacgctaggt acggaagata
      181 acgatattgt ttactttccc cttgaacaag gcctcaatca gtttttactt gatatacgtg
      241 aggaaggtgt tttcctttta tctccggttg aattttggat tgatgggcaa cccacgccct
      301 atgaagcaga taaaccatta cctgtcggaa agattattga tatagcggga tgttgcttca
      361 tcattggtga tattgaccat tccttgccat tgtctgatgt gccagaaagg ttttctgcca
      421 aaaatcaacg caaaaaacgc cttatcctgg cgagcgtgat tggtgctact tttgcgttgt
      481 ctggtgccat cggcagttac gttttgcttt ccccaaaagc tgaaccccca acgtttacca
      541 gagctgatgt atatcagcag ctaaaagaaa acaaacttca tgcaatcact ctggtatggc
      601 atggaaaaaa tatcgcgctt tatgggcgtt gcgaatcaac cac
//
LOCUS       DAZFJL010000358          642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFJL010000358 DAZFJL010000000
VERSION     DAZFJL010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.361"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            41..>642
                     /locus_tag="ACHJ8C_004851"
     CDS             41..>642
                     /locus_tag="ACHJ8C_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO5554527.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDE"
BASE COUNT          172 a          152 c          162 g          156 t
ORIGIN      
        1 tactttttta ttgccttcaa ataaatttaa ggagttcgaa atggaccaga agctgttaac
       61 ggatttccgc tcagaactac tcgattcacg ttttggcgca aaggccattt ctactatcgc
      121 ggagtcaaaa cgatttccgc tgcacgaaat gcgcgatgat gttgcatttc agattatcaa
      181 tgatgaatta tatcttgatg gcaacgctcg tcagaacctg gccactttct gccagacctg
      241 ggacgacgaa aacgtccata aattgatgga tttgtcgatc aataaaaact ggatcgacaa
      301 agaagaatat ccgcaatccg ctgcaatcga cctgcgttgc gtaaacatgg ttgccgatct
      361 gtggcatgcg cctgcaccga aaaatggtca ggccgttggc accaacacca ttggttcttc
      421 cgaggcctgt atgctcggcg ggatggcgat gaaatggcgt tggcgcaagc gtatggaagc
      481 cgcaggcaaa ccaacggata aaccaaacct ggtgtgcggt ccggtacaaa tctgctggca
      541 taaattcgcc cgctactggg atgtggagtt gcgtgagatc cctatgcgcc ccggtcagtt
      601 gtttatggac ccgaaacgca tgattgaagc ctgcgacgaa aa
//
LOCUS       DAZFJL010000359          640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFJL010000359 DAZFJL010000000
VERSION     DAZFJL010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.362"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..606)
                     /locus_tag="ACHJ8C_004852"
     CDS             complement(<1..606)
                     /locus_tag="ACHJ8C_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO5554528.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACVTLWQSPLPNLF
                     VVPVSLLFTIIAVHYTGKVVAHWIPRDHSSAITEEEYVGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKTFTKGDKVLIICRLSATRYLAEN"
BASE COUNT          205 a          120 c          152 g          163 t
ORIGIN      
        1 attttccgcc aaatatcgcg ttgcagaaag tcggcaaata attaacactt tgtctccttt
       61 agtgaaagtt tttccttctt caggctccag tagcaaataa tgtatttggc caaattgatc
      121 ggtgagtttt ccttcgcaag ggttacctga agtggcttga tggccggtaa ttaatgccat
      181 gctaccaaca tattcttctt ctgtaatagc tgaactatgg tcgcgtggaa tccagtgtgc
      241 aacgacttta ccggtataat gcactgcaat aattgtaaac agtaaactga caggaaccac
      301 gaacaggttg gggagtggtg attgccagag cgtaacgcag gcatgttgca acaggatgcc
      361 aataagacca aaaaaaccag cgagtaagca gaggacgacg agagctggta atcttccaat
      421 attgagataa tgaagtgcct ggctaatatg acctgtggtt atggaatcat aatggtcaag
      481 atgtgcatca agagcaccgg agagcatgtg accgcagata agtgcaaaaa tttctaaaag
      541 gccgatcaag agtacaaagg aaatagcgaa aagataaggg gtgttatagt cggcgaataa
      601 aatcatatgt cactccaatg tccatttagt gacgcaatat
//
LOCUS       DAZFJL010000360          640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFJL010000360 DAZFJL010000000
VERSION     DAZFJL010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.363"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>640
                     /locus_tag="ACHJ8C_004853"
     CDS             <1..>640
                     /locus_tag="ACHJ8C_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO5554529.1"
                     /translation="PGGAACRYNDIIPADHCLHDVQDMSTLNHPRADLSKGQYGCVGQ
                     GLHIAKKLLPYIPNNAGILLVPCCRGGSAFTQGAEGTFSESTGASQDSARWGVGKPLY
                     QDLISRTKAALQKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLSVFN
                     AQCHGGSAADVPWVCGDTTYYWKNTYATQYDTVYGGYKNRESEGVYFVPFMT"
BASE COUNT          144 a          159 c          194 g          143 t
ORIGIN      
        1 cgccgggcgg ggctgcctgc agatataacg atattattcc ggctgaccac tgtctgcatg
       61 atgtgcagga tatgagtacg ctgaatcatc cgagggctga cctgagcaaa gggcagtacg
      121 gctgtgtcgg ccagggttta catattgcca aaaaactgct cccgtatatc ccgaataacg
      181 cggggatcct gctggtacca tgctgtcgtg gtggttcggc atttacccag ggcgcggagg
      241 ggacattcag cgagtccacg ggggccagtc aggattcggc acgctggggg gtgggcaagc
      301 cgttatatca ggatctgatt tcccgcacaa aagcggcatt gcagaaaaat cccaaaaacg
      361 ttctgctggc cgtctgctgg atgcagggtg agtttgacat gagtgccgcc acccacgcac
      421 agcaacctgc gctgtttaca gccatgctga cacagtttcg tgctgacctc tccgtgttta
      481 acgcgcagtg ccatggtggc agtgctgcag atgtgccgtg ggtttgtggt gacacgacgt
      541 attactggaa aaatacatac gctacccagt acgacaccgt gtacggcggg tataaaaaca
      601 gggagagtga gggcgtttat tttgtgccct tcatgacaga
//
LOCUS       DAZFJL010000361          640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFJL010000361 DAZFJL010000000
VERSION     DAZFJL010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.364"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            88..>640
                     /locus_tag="ACHJ8C_004854"
     CDS             88..>640
                     /locus_tag="ACHJ8C_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HFO5554530.1"
                     /translation="MPVITGNLGQGKGIVAAYFASLYYRRGLWVAANYPLNTECMSSG
                     SDNPVTVIPAMPRIEDLELLGRGCPENEKTRFGALFLDECATWLNTRGFARKDRLPLI
                     DWLIHSRKLGWDVYLIAQHEDMIDSQIIKAMGAKIIRCRRLDELRVPVITPLMELFRP
                     GKTGIASGKRGIIPHYVTASTFLY"
BASE COUNT          153 a          118 c          169 g          200 t
ORIGIN      
        1 ttataaggac agggtgtatc gtttgattta tgatttgttt aaatcaaatg gactttctgt
       61 tggtaaataa tcatgcgggg ataaaacatg ccggttatta caggtaatct gggtcagggt
      121 aaaggcattg tggcggctta ttttgcctcg ctttattatc gccgtgggtt gtgggttgct
      181 gcaaattacc cgcttaacac tgagtgcatg tcttccggtt cggataatcc ggttacggtt
      241 attcccgcca tgccgcgaat tgaagacctg gaacttctcg gtcgtggatg tcctgaaaat
      301 gaaaaaacac gcttcggggc gttatttctt gatgagtgtg cgacatggtt gaatacccgt
      361 ggttttgccc gtaaggacag attaccgtta attgactggc tgatacattc ccgtaagctg
      421 ggctgggatg tttatctgat tgcacagcat gaagatatga ttgattccca gataattaag
      481 gcgatggggg ctaaaattat tcgctgccgc cgtcttgatg aattacgtgt tcctgtgatt
      541 acgccgctga tggaattatt cagaccagga aaaacgggga ttgcatcggg taaaaggggg
      601 attattcctc attatgttac cgccagtacg tttctttatg
//
LOCUS       DAZFJL010000362          625 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZFJL010000362 DAZFJL010000000
VERSION     DAZFJL010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.365"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..135
                     /locus_tag="ACHJ8C_004855"
     CDS             <1..135
                     /locus_tag="ACHJ8C_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO5554531.1"
                     /translation="RVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNALKNLNF
                     "
     gene            150..>625
                     /locus_tag="ACHJ8C_004856"
     CDS             150..>625
                     /locus_tag="ACHJ8C_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO5554532.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVEAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEALDRNILNLLKDEVVMRQEATVITVGGSDYKKLVNLGG"
BASE COUNT          170 a          141 c          199 g          115 t
ORIGIN      
        1 agagtcctgc gtgatgtcgg actctcccgt acccaggcca aagcattcat ggccgggggc
       61 tatggcgcac tgtccctgcg cgacgctgag gatgtgggct ctgcactgaa tgcactgaaa
      121 aatctgaact tctaatcagg agaaatacga tggcggttga tattaaagat gtcgaacagg
      181 tcgcgcagga gctgcagcag aagtttgacg acttcaaggc aaagaacgac aagcgcgtgg
      241 aggcgattga gcaggaaaaa ggcaagcttg ccgggcaggt ggaaaccctg aacgggaaac
      301 tcagtgagct ggaaaatctc aaaagcgacc ttgaaaaaga gctgcttgag ctgaaacgtc
      361 cggcaggtgg tgcgcaaaat aaactggcca ccgagcataa agaagcgttt gtgggcttcc
      421 tgcgtaaagg ccgtgaagac ggtctgcgcg atctggagcg taaggcattg caggtgggca
      481 ccgatgaaga cggtggctat gccgtgccgg aagcactgga tcgcaacatt ctcaacctgc
      541 tgaaagatga agtggtgatg cgccaggaag ccacggtgat caccgttggt ggttccgact
      601 acaaaaaact ggtgaatctg ggcgg
//
LOCUS       DAZFJL010000363          611 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFJL010000363 DAZFJL010000000
VERSION     DAZFJL010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.366"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(316..>611)
                     /locus_tag="ACHJ8C_004857"
     CDS             complement(316..>611)
                     /locus_tag="ACHJ8C_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5554533.1"
                     /translation="NLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGISFQSILDD
                     VRLNNALSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          174 a          135 c          105 g          197 t
ORIGIN      
        1 tatcaattag atttaataac aacgatatcc gtacctaacc ggatatctaa tgtcataagc
       61 atccctttaa ttatcccctc aacctcttgt aactttccca ctcttgaact ccacatagtc
      121 caaacgccgg atgcaaaaaa atcacacgtg catttctgaa attgtgatga aggaagatag
      181 tatcaattat atgtgcttat aaacgtcccc gattagcgca gaagtcaccg gattgttcag
      241 gctccaatag gttgagtatg ccgtatttat tttccaaaat aaaaatcgat gctgttgctc
      301 cttcataaaa tacttttact ctctggacgc ttttcttagc tctcttggtg ttatcttaaa
      361 acgattatgg aacctttcag taaaacgaga aggacacttg taaccatttt ccctggcaat
      421 ctcgctgatg ggttttactg tcgtttgtat agcagacaac gcattattta acctcacgtc
      481 gtccaatata ctttgaaaac ttattccctc gcttgccaga cggcgatgca atgtggaaac
      541 agaaatgtag agatatctcg ctaccttgtt tgctgtccat tttgttccag gttcggatag
      601 cattaggtta t
//
LOCUS       DAZFJL010000364          606 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFJL010000364 DAZFJL010000000
VERSION     DAZFJL010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.367"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>606
                     /locus_tag="ACHJ8C_004858"
     rRNA            <1..>606
                     /locus_tag="ACHJ8C_004858"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          167 a          132 c          185 g          122 t
ORIGIN      
        1 atggggtgac tgcgtacctt ttgtataatg ggtcagcgac ttatattctg tagcaaggtt
       61 aaccgaatag gggagccgaa gggaaaccga gtcttaactg ggcgttaagt tgcagggtat
      121 agacccgaaa cccggtgatc tagccatggg caggttgaag gttgggtaac actaactgga
      181 ggaccgaacc gactaatgtt gaaaaattag cggatgactt gtggctgggg gtgaaaggcc
      241 aatcaaaccg ggagatagct ggttctcccc gaaagctatt taggtagcgc ctcgtgaatt
      301 catctccggg ggtagagcac tgtttcggca agggggtcat cccgacttac caacccgatg
      361 caaactgcga ataccggaga atgttatcac gggagacaca cggcgggtgc taacgtccgt
      421 cgtgaagagg gaaacaaccc agaccgccag ctaaggtccc aaagtcatgg ttaagtggga
      481 aacgatgtgg gaaggcccag acagccagga tgttggctta gaagcagcca tcatttaaag
      541 aaagcgtaat agctcactgg tcgagtcggc ctgcgcggaa gatgtaacgg ggctaaacca
      601 tgcacc
//
LOCUS       DAZFJL010000365          604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFJL010000365 DAZFJL010000000
VERSION     DAZFJL010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.368"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..>604)
                     /locus_tag="ACHJ8C_004859"
     CDS             complement(<1..>604)
                     /locus_tag="ACHJ8C_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFO5554534.1"
                     /translation="ALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIER
                     SALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVY
                     HQEQVNGPLVDESGRVIFADFNPSKRPWSMASDDSNNSWNPAYNCFDRPGKKCISFTL
                     HINGKDHDLLAVDKIHVDLNWRYLNEYLDHISANDEVLFLK"
BASE COUNT          186 a          129 c          111 g          178 t
ORIGIN      
        1 gctttaaaaa tagaacttca tcattagcgc tgatgtgatc aagatactcg ttcagatatc
       61 gccagtttaa atcgacatga attttatcta ccgctaacaa atcatgatct ttgccgttga
      121 tgtgtagcgt aaaagagata cattttttac ccggacgatc aaagcaattg tatgccggat
      181 tccagctgtt gttagagtca tctgaagcca tcgaccacgg tcgtttcgat gggttaaaat
      241 cggcaaaaat aacccgccct gactcgtcga caagcggtcc atttacttgt tcctgatgat
      301 aaacaacgat tttatcattc gccctccgcg aaaagagata atatttatta tcatcaagta
      361 ccagtcccac cgagtctaaa aatggcatca tttccatttt cagaatcacg gcacgttgaa
      421 tctcctctgt attttttaat aaatacagtg agtcctttaa tgtcagggcc gaacgttcaa
      481 tatctttaaa gaaatagcca acgtttttct gaataagttc aaagcggcta tcggcacgtt
      541 ttaccgcgcg ttccgatacc agttgatagt taatgatgaa aaaaagacca ataaatgaca
      601 gtgc
//
LOCUS       DAZFJL010000366          602 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFJL010000366 DAZFJL010000000
VERSION     DAZFJL010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.369"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>602
                     /locus_tag="ACHJ8C_004860"
     CDS             <1..>602
                     /locus_tag="ACHJ8C_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO5554535.1"
                     /translation="HNASVSNPKNRIAELCVRGKAVCELSEDMGLSGYFIVLTTPSRF
                     HPTTSFKVAGKWHSRPNKKWWEAGCPTIKDSHVWLNTVWRRVCRRLDKAGIQIPGLRT
                     VEPHADGTTHWNFLIYCNPHESATVLAIFREEAMRDEPDEKGAKEHRIRIEAIDPEKG
                     DGFRYIVKYITKMAGDASADGIASLNDRYSARSFCDAVSRA"
BASE COUNT          137 a          162 c          170 g          133 t
ORIGIN      
        1 cataacgcct ctgtttcgaa cccgaaaaac cggattgctg aactctgcgt tcgtggaaag
       61 gccgtctgtg aactgtccga ggatatgggg ctgtccggct acttcatcgt gctcaccacg
      121 ccgtcacgtt ttcacccgac aaccagtttt aaggttgccg gaaagtggca ttcccgcccg
      181 aacaagaaat ggtgggaagc aggttgcccg accattaagg attcgcacgt atggctgaat
      241 accgtctggc ggcgggtctg ccgcagactg gataaagccg gtattcagat acccggtctg
      301 cgcacagttg agcctcatgc tgacggcaca acacactgga atttcctgat ttactgcaat
      361 ccccatgaga gcgcaacggt gctggccatt ttccgtgaag aggccatgcg cgatgagccg
      421 gatgaaaagg gggcgaaaga gcaccgtatc cgtattgagg ctattgaccc tgaaaagggc
      481 gacggtttcc gttacatcgt gaagtacatc accaaaatgg cgggcgatgc cagtgcggac
      541 ggtattgcct ccctgaatga ccgttattct gcccgttcat tctgtgacgc ggtcagccgt
      601 gc
//
LOCUS       DAZFJL010000367          598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFJL010000367 DAZFJL010000000
VERSION     DAZFJL010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.370"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>598
                     /locus_tag="ACHJ8C_004861"
     rRNA            <1..>598
                     /locus_tag="ACHJ8C_004861"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          167 a          131 c          188 g          112 t
ORIGIN      
        1 gaatgctgac ataagtaacg ataaagcggg tgaaaagccc gctcgccgga agaccaaggg
       61 ttcctgtcca acgttaatcg gggcagggtg agtcgacccc taaggcgagg ccgaaaggcg
      121 tagtcgatgg gaaacaggtt aatattcctg tacttggtgt tactgcgaag gggggacgga
      181 gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag
      241 gcaaatccgg aaaaccaagg ctgaggcgtg atgacgaggc actacggtgc tgaagcgaca
      301 aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac
      361 cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact
      421 aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tatgtaggtg aagcgacttg
      481 ctcgtggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca
      541 gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaag
//
LOCUS       DAZFJL010000368          592 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFJL010000368 DAZFJL010000000
VERSION     DAZFJL010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.371"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            45..>592
                     /locus_tag="ACHJ8C_004862"
     CDS             45..>592
                     /locus_tag="ACHJ8C_004862"
                     /inference="COORDINATES: protein motif:HMM:NF017518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HFO5554536.1"
                     /translation="MSSGSDNPVTVIPAMPRIEDLELLGRGCPENEKTRFGALFLDEC
                     ATWLNTRGFARKDRLPLIDWLIHSRKLGWDVYLIAQHEDMIDSQIIKAMGAKIIRCRR
                     LDELRVPVITPLMELFRPGKTGIASGKRGIIPHYVTASTFLYDGTAHAARRPVDKVVI
                     KAADYYNVYDTNFIFSDGMELL"
BASE COUNT          141 a          127 c          150 g          174 t
ORIGIN      
        1 gtgggttgcg ggttgctgca aattacccgc ttaacactga attcatgtct tccggttcgg
       61 ataatccggt tacggttatt cctgccatgc cgcgaattga agaccttgaa cttctcggac
      121 gtggatgccc tgaaaatgaa aaaacacgct tcggggcgtt atttcttgat gagtgtgcga
      181 catggcttaa tacccgtggc tttgcccgta aggaccgact accgttaatt gactggctga
      241 tacattcccg gaagctgggc tgggatgttt atctgattgc acagcatgaa gacatgattg
      301 attcccagat aattaaggcg atgggggcta aaattattcg ctgccgtcgt cttgatgaat
      361 tgcgcgttcc tgtgattaca ccgctgatgg aattattcag accaggaaaa acggggattg
      421 catcggggaa aagggggatt attcctcatt atgttaccgc cagtacgttt ctttatgacg
      481 gcaccgctca tgctgcccga cgtcctgttg ataaggttgt tattaaggcc gctgattatt
      541 acaacgttta tgacaccaat tttattttct ctgacggaat ggaactgctt aa
//
LOCUS       DAZFJL010000369          558 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZFJL010000369 DAZFJL010000000
VERSION     DAZFJL010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.372"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            149..493
                     /gene="yoaB"
                     /locus_tag="ACHJ8C_004863"
     CDS             149..493
                     /gene="yoaB"
                     /locus_tag="ACHJ8C_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO5554537.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT          158 a          117 c          148 g          135 t
ORIGIN      
        1 attcaattag caactaacat agttgctgtt gatttgaaca gggattatgt caggatgagg
       61 gcgctttcgc cagttgaagt ggtgacggcg acctcacatt gtggcagtct ttgcagcacg
      121 aaaatggaag tataatgagg acaaaatgat gactatcgtt cgtatcgatg ctgaagcccg
      181 ctggtctgat gtagtaatcc acaacaacac gctctactac actggtgtac cggaaaacct
      241 cgacgccgat gcctttgagc aaaccgccaa cacgctggcg cagattgacg ccgtgctgga
      301 aaaacagggc agcaataaat cgagcattct ggatgccacc attttcctgg ccgataaaaa
      361 cgacttcgcg gcgatgaata aagcgtggga tgcctgggtt gtcgcgggtc atgcgccggt
      421 gcgctgcacg gtacaagcgg gtttgatgaa cccgaagtat aaagttgaaa ttaagattgt
      481 ggctgcggtg taagctttat cgaagcaaaa taagtcagac gataatttat cgataatact
      541 ggtcggtttt acataaat
//
LOCUS       DAZFJL010000370          557 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZFJL010000370 DAZFJL010000000
VERSION     DAZFJL010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.373"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            25..>557
                     /locus_tag="ACHJ8C_004864"
     CDS             25..>557
                     /locus_tag="ACHJ8C_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysogenic conversion protein"
                     /protein_id="HFO5554538.1"
                     /translation="MILAMVLFIGNIFYTNHRDDISMEAERDSIRTMFAYEIANNHRA
                     LTFLDKTRNIGFDENSEHFVGEPFAINVKSLGGPRLQIALNQTDKVFKAYFSELSKLD
                     KEDVTLLMDYYHEQSILLECVKSTLQKMKSSNDIKVDIDGYLLEEHFMNEFNLSNILL
                     KRYSHLLSQHPKKPETK"
BASE COUNT          188 a           86 c          106 g          177 t
ORIGIN      
        1 aatattaatt gggaggcaag ttcagtgatt cttgctatgg ttctctttat tggaaatata
       61 ttttatacga atcatcgtga tgatataagc atggaggctg agagagacag tatcagaaca
      121 atgtttgcat atgaaatcgc taataaccat cgcgctctca cttttcttga taaaacgaga
      181 aatattggct ttgacgaaaa ttcggagcat tttgttggcg agccttttgc cattaatgtc
      241 aaatcattag gagggcctcg cttacagatt gcattaaacc agactgataa agtttttaaa
      301 gcctacttca gtgaattaag taaacttgat aaagaggatg ttactcttct tatggactat
      361 taccatgagc aaagcatcct gctggagtgt gtaaaatcta cgttacagaa gatgaaaagt
      421 agtaatgata ttaaagttga tattgatggt tacttattag aagaacactt catgaatgag
      481 tttaatcttt ctaatatttt gcttaaacgc tatagccatt tgttgtcaca acacccaaaa
      541 aaacctgaaa caaaaga
//
LOCUS       DAZFJL010000371          554 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFJL010000371 DAZFJL010000000
VERSION     DAZFJL010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.374"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>554
                     /gene="escU"
                     /locus_tag="ACHJ8C_004865"
     CDS             <1..>554
                     /gene="escU"
                     /locus_tag="ACHJ8C_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFO5554539.1"
                     /translation="KNNLKNIFSVKSIFELLKSVFKLVIIVLIFYFMGHSYANEFAYF
                     TGLNAYQALVVVAFFVFLLWKGVLFGYLLFSVFDFWFQKHEGLKKMKMSKDEVKREAK
                     DTDGNPEIKGERRRLHSEIQSGSLANNIKKSTVIVKNPTHIAICLYYKLGETPLPLVI
                     ETGKDAKALQIIKLAELYDIPVIE"
BASE COUNT          177 a           89 c          109 g          179 t
ORIGIN      
        1 aaaaaataac ctgaaaaata ttttttctgt aaagagcatt tttgagctac ttaaatcagt
       61 atttaagtta gtgataattg ttctcatttt ttattttatg gggcattcat atgcaaatga
      121 gtttgcttat ttcacaggac tgaacgcata tcaagctctt gtcgttgttg ccttttttgt
      181 ttttctttta tggaaaggcg tgctattcgg atatctactc ttttcagtat ttgatttctg
      241 gttccagaag catgagggac tgaagaaaat gaaaatgagt aaagatgagg tgaaacgaga
      301 agccaaggat actgatggta accctgaaat taaaggggag cgccgtcgcc ttcattccga
      361 gatacaaagt ggaagtttgg ctaataacat caaaaaatca accgttattg ttaaaaaccc
      421 gactcacatt gcgatttgcc tatactataa acttggggag actccattac ctttagttat
      481 tgaaacagga aaagatgcca aagctctaca gatcattaaa ctggctgaac tctatgatat
      541 tccagtgatt gaag
//
LOCUS       DAZFJL010000372          539 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFJL010000372 DAZFJL010000000
VERSION     DAZFJL010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.375"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            321..>539
                     /locus_tag="ACHJ8C_004866"
     CDS             321..>539
                     /locus_tag="ACHJ8C_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554540.1"
                     /translation="MILKINQQKLVFAWGVVLLVVRTTNLLSLFVEWVKLFTDKVTRG
                     GKMKKWMLAICLMFINEICHATDCFDLAG"
BASE COUNT          175 a          109 c          104 g          151 t
ORIGIN      
        1 tttttcataa gcatcattgc tgatatacag attcacctta gccattagat attacctttt
       61 ttaaacataa aacaaagtta atttcgaaac actagaattc atagtacaaa caggggcgca
      121 ccgctagcag cgcccctgac ggtatcctat aaaaaaacac accgcgccgc tagcagcacc
      181 cctaatataa aataatgttt tttataaaaa tagtcagcac cacccctaca aagcggtgtc
      241 ggcgcgttgc tgtagctgcg ttaacgacgc tgctttaaat aaatcagatt taaacaatat
      301 aaatccacaa atacaactca atgatattaa agataaatca gcaaaagctt gtttttgcgt
      361 ggggcgtggt gcttttggtg gtgagaacca ccaacctgtt gagccttttt gtggagtggg
      421 ttaaattatt tacggataaa gtcaccagag gtggaaaaat gaaaaaatgg atgttagcca
      481 tctgcctgat gtttataaat gagatctgcc atgccactga ttgctttgat cttgcaggc
//
LOCUS       DAZFJL010000373          530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZFJL010000373 DAZFJL010000000
VERSION     DAZFJL010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.376"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..333
                     /locus_tag="ACHJ8C_004867"
     CDS             <1..333
                     /locus_tag="ACHJ8C_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015387350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554541.1"
                     /translation="TSGSSNGSYSNLGSHRGSFTGRNTGSGTLFVYASGGNGGSAGGD
                     CANTSRLQGYVAGALISTNASNNPSYGKTAFISFAVPAGATYQITSYPAQNYSCGSGV
                     FSVFGYQT"
     gene            complement(330..>530)
                     /locus_tag="ACHJ8C_004868"
     CDS             complement(330..>530)
                     /locus_tag="ACHJ8C_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin"
                     /protein_id="HFO5554542.1"
                     /translation="ALGGKLKVTQLSSTGYLGQFDFCAIARMGNAEDSHYCQVVESPS
                     GSRKWYKYEHKTGCIASCVTLN"
BASE COUNT          135 a          128 c          122 g          145 t
ORIGIN      
        1 acttccggtt cctctaacgg tagttacagt aatcttggct cacacagagg atcatttact
       61 ggtcgcaata caggaagtgg tacgctgttt gtttacgcct ctggagggaa tggtggttcc
      121 gccgggggag attgcgctaa cacatccaga ttgcagggat acgttgcagg ggcattgatt
      181 agcacaaatg cgagcaacaa cccatcttat gggaagacag cattcatctc ttttgctgtc
      241 cctgctggcg caacttatca gatcacctct tatccggccc aaaactactc ttgtggctca
      301 ggggtatttt cagtatttgg ataccagact taattgagag ttacacagga cgcaatacaa
      361 cctgtcttgt gctcgtattt gtaccatttt cgtgaaccag atggactctc gactacctgg
      421 cagtaatgag aatcctcagc atttcccatt ctggcaatgg cacaaaagtc aaattgcccc
      481 aagtaaccag tgctggaaag ttgagtaacc ttgagctttc cacctaatgc
//
LOCUS       DAZFJL010000374          527 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZFJL010000374 DAZFJL010000000
VERSION     DAZFJL010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.377"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..275
                     /locus_tag="ACHJ8C_004869"
     CDS             <1..275
                     /locus_tag="ACHJ8C_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFO5554543.1"
                     /translation="KLAQVIAKLLPLQLLRGVGLEQRQHVHLARKSLVEAVNADQIPD
                     LYGSRVPCLQATMHGLPVATRPKIKMVQLQFTPFASDDTYHWQTTM"
     gene            complement(254..>527)
                     /locus_tag="ACHJ8C_004870"
     CDS             complement(254..>527)
                     /locus_tag="ACHJ8C_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shufflon protein D'"
                     /protein_id="HFO5554544.1"
                     /translation="RASGSTVLTGKIANGQQIPLPSGFSASQCTWSVSNAENPHGWKP
                     NYFAGSVATYDANRIVKCGFYDEYNFYGGTHRTDLSGKCSYIVVCQ"
BASE COUNT          140 a          131 c          121 g          135 t
ORIGIN      
        1 aaaaattggc gcaggtgata gccaaattgt taccgcttca gctactgcgt ggcgttggcc
       61 tggagcaacg gcaacatgtc catctggcaa gaaagtcatt ggtggaggcg gtcaatgcag
      121 atcaaatacc ggatttatat ggctcacgcg ttccatgcct tcaggcaaca atgcatggac
      181 tgccagttgc gacacgaccg aagatcaaaa tggttcaatt acagtttacg ccatttgcca
      241 gtgacgatac ttatcactgg caaaccacga tgtaactaca tttccccgac aggtcagttc
      301 ggtgagtacc gccataaaag ttgtattcgt catagaagcc gcatttcaca attcgattgg
      361 catcataggt tgcaactgac cccgcaaaat aattcggctt ccacccgtgc ggattttctg
      421 cattactgac actccaggta cactggctgg cagaaaaacc agatggcaat gggatctgtt
      481 gtccatttgc tatttttcct gtcagaacag tgctacctga tgccctt
//
LOCUS       DAZFJL010000375          516 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZFJL010000375 DAZFJL010000000
VERSION     DAZFJL010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.378"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>516
                     /locus_tag="ACHJ8C_004871"
     rRNA            <1..>516
                     /locus_tag="ACHJ8C_004871"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          115 a          112 c          178 g          111 t
ORIGIN      
        1 tggtgggtag tttgactggg gcggtctcct cctaaagagt aacggaggag cacgaaggtt
       61 ggctaatcct ggtcggacat caggaggtta gtgcaatggc ataagccagc ttgactgcga
      121 gcgtgacggc gcgagcaggt gcgaaagcag gtcatagtga tccggtggtt ctgaatggaa
      181 gggccatcgc tcaacggata aaaggtactc cggggataac aggctgatac cgcccaagag
      241 ttcatatcga cggcggtgtt tggcacctcg atgtcggctc atcacatcct ggggctgaag
      301 taggtcccaa gggtatggct gttcgccatt taaagtggta cgcgagctgg gtttagaacg
      361 tcgtgagaca gttcggtccc tatctgccgt gggcgctgga gaactgaggg gggctgctcc
      421 tagtacgaga ggaccggagt ggacgcatca ctggtgttcg ggttgtcatg ccaatggcac
      481 tgcccggtag ctaaatgcgg aagagataag tgctga
//
LOCUS       DAZFJL010000376          506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZFJL010000376 DAZFJL010000000
VERSION     DAZFJL010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.379"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..>506)
                     /locus_tag="ACHJ8C_004872"
     CDS             complement(<1..>506)
                     /locus_tag="ACHJ8C_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFO5554545.1"
                     /translation="IVLLIAILASIVIIFNKPIQNRYNEALNDLNSYTNANSVTSLGA
                     RLAMYEIGLNIFTKSPFSFRSAESRAENMNLLVAEHNRLRGALEFSNVHLHNEIIEAG
                     SLKGLMGIISILFLYFSLFYTAYKRRALGLLILTLGIVGIGLSDVIIWARSIPIIVIS
                     AIVLLLVI"
BASE COUNT          169 a           96 c           86 g          155 t
ORIGIN      
        1 aataacgagt aagaggacta tagcggatat aacgataatt ggaatgctgc gtgcccatat
       61 gatcacatca ctgagtccaa tccccacaat gccaagcgtt aatatcaaca aacccaaagc
      121 tcgtctttta tatgctgtat aaaacagtga aaaatagagg aaaaggatgg aaataattcc
      181 catcagacct ttcagtgacc ctgcttcaat tatctcatta tgtagatgta cgttagaaaa
      241 ctccaatgcc cctcttagcc tgttgtgttc tgcaactaac aaattcatat tttcagcgcg
      301 tgactctgct gatctaaatg aaaaaggtga ctttgtgaat atatttaaac caatttcgta
      361 cattgccagt cttgcaccta gggaagtcac gctattagca ttggtataac tgtttaaatc
      421 atttaatgct tcattatagc gattctgtat tggtttatta aatataatta ctatgctagc
      481 taatatagca attagtagaa caatag
//
LOCUS       DAZFJL010000377          485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFJL010000377 DAZFJL010000000
VERSION     DAZFJL010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.380"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>485
                     /locus_tag="ACHJ8C_004873"
     CDS             <1..>485
                     /locus_tag="ACHJ8C_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFO5554546.1"
                     /translation="KHMNVWVTAESAWMDMIKWEKCEYIAPRHELKTYPMWVGVDLAH
                     KIDICAAAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAELYRKWAEMDKLILTDGDV
                     IDHAQIKSDLLEWIGGENLRELGFDPWSAMQFSLALAEEGIPLVEVPQTVRNLSEAMK
                     ET"
BASE COUNT          127 a           99 c          152 g          107 t
ORIGIN      
        1 aaacacatga atgtgtgggt cactgccgag tctgcctgga tggacatgat taagtgggag
       61 aaatgcgaat atattgcccc acgacatgag ctgaaaacgt atcccatgtg ggtcggcgtt
      121 gaccttgctc ataagattga tatctgtgcg gcggcaaaac tctggcgaac ggataacggg
      181 catgttcatg ccgattttaa attctggctc ccggaaggac ggctggaacg atgctcgcgg
      241 cagcaggcag aactttaccg gaagtgggcg gagatggata agctgattct gacggatggt
      301 gatgttatcg atcatgctca gataaaaagt gacttactgg aatggattgg tggtgaaaac
      361 ctcagggaac tgggatttga cccgtggagc gcgatgcagt tcagcctggc actggctgaa
      421 gaagggatac cactggtgga ggttccgcag acggtccgca atctgtcaga ggccatgaag
      481 gaaac
//
LOCUS       DAZFJL010000378          482 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFJL010000378 DAZFJL010000000
VERSION     DAZFJL010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.381"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..482
                     /locus_tag="ACHJ8C_004874"
     rRNA            <1..482
                     /locus_tag="ACHJ8C_004874"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          118 c          142 g          103 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcacctcc
      481 tt
//
LOCUS       DAZFJL010000379          475 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFJL010000379 DAZFJL010000000
VERSION     DAZFJL010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.382"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
BASE COUNT          160 a           97 c           84 g          134 t
ORIGIN      
        1 tcccaaaagg aatcctcatg aataagatca tcacatttat cattgcagct attatcttat
       61 ttagttggtt aattataaac agcacaacta agcaaaaaaa tatttcatag ctaatgtacc
      121 ttttactatt aagtctaacc taccaggcac atgtagcgta agtacaatga caacgtctac
      181 agataccaaa cagaatatac ctgtcgtagc gtgcaaagtc agtattacga ttgcattcac
      241 ttccagtaat actatttact aatcacggag cttactttgt caacgaactt ggatgcaaaa
      301 acgccaaaca ccgcctcaat cgccccagaa cgtttgagtt cggaatggag tgcctctgaa
      361 ggttgagtaa gtctacaggc atatgagggc aaatgaatct gcatttggtt tacatgaata
      421 ctaatatggc ctttttcgaa agcgaggcaa acagatttgc agctgaactg ttaat
//
LOCUS       DAZFJL010000380          474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZFJL010000380 DAZFJL010000000
VERSION     DAZFJL010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.383"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(97..>474)
                     /locus_tag="ACHJ8C_004875"
     CDS             complement(97..>474)
                     /locus_tag="ACHJ8C_004875"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO5554547.1"
                     /translation="NVKNKGIIVIGSNCRFSNNVHIACINRITIGKGVLIGSNVLITD
                     HSHGKLVAEEKFISPYERTLFSKGEVIIGDNVWICDGVMILPGVTIGSGAIIAANSVV
                     VSNIPSFTVAAGTPAKVIKCIGA"
BASE COUNT          159 a          108 c           64 g          143 t
ORIGIN      
        1 tacataatac aatcaactct attttaaaat ttcctcccca aagcacaatt ccagggaaaa
       61 aaacagttaa taaaataagt attattgaag tggttctcac gccccaatgc atttaataac
      121 cttagcaggt gttcctgccg caacagtaaa cgatggtatg ttgctaacaa caacggaatt
      181 agcagctata atagctccag agccaatcgt aactcctggc aagatcataa caccatcgca
      241 tatccataca ttgtcaccaa taatgacctc tcccttagaa aagagagtac gctcataagg
      301 gctaataaat ttttcttcag cgacaagttt cccatgacta tgatccgtaa ttaatacatt
      361 actacctatt agcacccctt tgccaattgt tattcggttt atgcacgcta tatgaacgtt
      421 attactaaac ctacaattag agcctattac aattattcct ttgtttttta catt
//
LOCUS       DAZFJL010000381          464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZFJL010000381 DAZFJL010000000
VERSION     DAZFJL010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.384"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..209)
                     /locus_tag="ACHJ8C_004876"
     CDS             complement(<1..209)
                     /locus_tag="ACHJ8C_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFO5554548.1"
                     /translation="MFSIRSLLPINASVSVPTNQSQSILTTLAGRTIEKAQEKEGLIV
                     FLGMKSVNEYTLNILGQNVSRVTAG"
BASE COUNT          133 a          100 c           79 g          152 t
ORIGIN      
        1 tttcccgctg tgactcttga aacattttga ccaagaatat taagagtata ttcattaacg
       61 gatttcattc ctaaaaaaac aattaatcct tctttctctt gagctttttc gattgttctc
      121 cctgctagag ttgttaggat ggattgagat tggtttgtcg gaactgatac gctggcgtta
      181 ataggtagta gggatcttat gctaaacata caacctcttt cttaatttgc gaacatgttt
      241 aggattctgt gtaaatgtct tttctcagaa gtgaccatcc aggcggtcac cgaactcgat
      301 aataaagctg atcatagcca ttcgtcagtc cctcagcggc attgtacatt tctgtgacgc
      361 ggcctagatt gccagccaca ccactttttt acaaactcca tcatgcaaca aatccactac
      421 attggataaa actactaaat tttacttaat accacactaa taag
//
LOCUS       DAZFJL010000382          452 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZFJL010000382 DAZFJL010000000
VERSION     DAZFJL010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.385"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            47..361
                     /locus_tag="ACHJ8C_004877"
     CDS             47..361
                     /locus_tag="ACHJ8C_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5554549.1"
                     /translation="MLHDHVAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLTEIKQAVNRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSK
                     KC"
BASE COUNT          152 a           77 c          128 g           95 t
ORIGIN      
        1 gaaggtaaat tcaggatggc agtctgtaga taatcggagg tcacttatgc tacatgatca
       61 cgtggcagaa tgtctggaga aaaaaggact gtaccggaga gcagctgaac gatgggcaaa
      121 agtgatggta cagctaagtg atgaccagaa aagaaaagtg gcggcacaga aacgagcaga
      181 gtgtttgcgt aaggcgcgcc ggactccggt ttcaccggtg aacctgaccg aaataaaaca
      241 agcggtcaac agactacatt ctgagttggg aatgggattt gaagagcggc gggtattccg
      301 acgatataaa gggacaggag aacagaatac gtccggaaac gcgcggtcaa aaaaatgcta
      361 aaaaatatct gagagagtta ttgcctgtta ccataagaaa aagcgacttt agtggtcgct
      421 ttttgtgtca tatataagtc gtttaagtaa ac
//
LOCUS       DAZFJL010000383          442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZFJL010000383 DAZFJL010000000
VERSION     DAZFJL010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.386"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            73..363
                     /locus_tag="ACHJ8C_004878"
     CDS             73..363
                     /locus_tag="ACHJ8C_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5554550.1"
                     /translation="MTYTVKFRDDALKEWLRLDKTIQQQFAKKLKKCSENPHIASAKL
                     RGLKDYCYKIKLRASGFRLVYQVIDDMLIIAVVAAGKHERSNVYHLASERMK"
BASE COUNT          134 a           94 c           91 g          123 t
ORIGIN      
        1 ccatgagctg gtaaaactgg ttactgaacg cagcagtcag cctctgcatg atgtggacct
       61 ggacagttat ttatgactta tacagtaaaa ttccgggatg atgcactgaa agaatggctc
      121 agactggaca agaccattca gcagcagttc gcaaaaaaac tgaaaaaatg cagtgaaaat
      181 cctcatatag cttctgcaaa actacgtgga ttaaaggact actgctataa aatcaaactt
      241 cgcgcatcag gttttcgtct ggtttatcag gttattgatg atatgttaat tattgcggtc
      301 gtcgcagcag gtaaacatga acgcagcaac gtttatcatc tcgccagtga acggatgaaa
      361 taatatcctg gtcagcactc tttcttacct gcttccattt tttcagtgat tcttttcctg
      421 catgcagaaa aaaagcctgc cc
//
LOCUS       DAZFJL010000384          440 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZFJL010000384 DAZFJL010000000
VERSION     DAZFJL010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.387"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>440
                     /gene="waaZ"
                     /locus_tag="ACHJ8C_004879"
     CDS             <1..>440
                     /gene="waaZ"
                     /locus_tag="ACHJ8C_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043842 - Kdo transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-D-manno-oct-2-ulosonate III transferase
                     WaaZ"
                     /protein_id="HFO5554551.1"
                     /translation="HQRRDDFYKFSQRSRYTIVNVDVYEHASKEDKLYILQNCLVLRS
                     FYRREKGGLIKKIKFNILSQIHKELLISVPLSKKGRLVGFCKDISLGYCSCHTIAFAA
                     IQIAYSLKYARIICSGLDLTGSCSRFYDENKNPMPSELSKDLFK"
BASE COUNT          134 a           81 c           81 g          144 t
ORIGIN      
        1 acatcagcgt cgtgatgatt tttataaatt tagccagaga agtcgttata ccatagtaaa
       61 cgttgacgtt tatgaacacg cttccaaaga agacaaactt tatatcctcc agaactgcct
      121 cgtattacga tctttttacc gccgagaaaa aggcggtttg ataaagaaga ttaaatttaa
      181 tattttgagc cagattcaca aagaactgct gatctctgta ccgttgtcta aaaaaggtcg
      241 tctggttgga ttttgcaagg acattagtct tggttattgc tcatgccata ctattgcctt
      301 cgctgcaatt caaatagcat attcacttaa atatgctcga attatttgtt ctggtcttga
      361 tttaacgggt agctgttctc gtttctatga cgaaaataag aatcccatgc cctcggaatt
      421 aagtaaggat ttattcaaaa
//
LOCUS       DAZFJL010000385          438 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZFJL010000385 DAZFJL010000000
VERSION     DAZFJL010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.388"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..240)
                     /locus_tag="ACHJ8C_004880"
     CDS             complement(<1..240)
                     /locus_tag="ACHJ8C_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554552.1"
                     /translation="MWAILGAFFVEIVLKSLRFIAGLIGDKAVAWARLISYLGLCYTS
                     YYALITAVNFIIRQISVVNKIDVITIAFSYLPLNIQ"
     gene            complement(240..>438)
                     /locus_tag="ACHJ8C_004881"
     CDS             complement(240..>438)
                     /locus_tag="ACHJ8C_004881"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554553.1"
                     /translation="LPFVLQFSFNLPLIGVIGFKVDTTDFAKAYDYYVRSILEYTLYM
                     MTMLRVFVITRRTLFEREVKS"
BASE COUNT          186 a           81 c           71 g          100 t
ORIGIN      
        1 ctgaatgttc agaggtaagt aactaaatgc aattgttata acatcaattt tattaaccac
       61 ggaaatttga cgaataataa aattaaccgc agtaataagt gcataatatg acgtataaca
      121 aagaccaaga taagaaataa gtctggccca tgcgacagcc ttatcgccaa tcaatccggc
      181 aataaaacgt agagatttta agacaatctc aacaaaaaaa gcgcctaaaa tagcccacat
      241 caggatttaa cctcacgttc aaaaagtgta cgacgcgtaa tcacaaaaac acgaagcatg
      301 gtcatcatat aaagggtgta ttcaagaatg gagcgaacat aataatcata agcctttgca
      361 aaatcagttg tgtcaacctt aaaaccaatc actccaatta aaggaaggtt aaaactaaat
      421 tgcaaaacaa aaggaagt
//
LOCUS       DAZFJL010000386          431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZFJL010000386 DAZFJL010000000
VERSION     DAZFJL010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.389"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>431
                     /locus_tag="ACHJ8C_004882"
     CDS             <1..>431
                     /locus_tag="ACHJ8C_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFO5554554.1"
                     /translation="SKTYDATGCFVGLEAWGDVSSERAIVRTRNISCLKDGKTIDMPI
                     KGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDGIGQGMERASQPAVGLGATAAYGA
                     GDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIPIGAGNEV"
BASE COUNT           99 a          100 c          146 g           86 t
ORIGIN      
        1 agcaaaacgt atgacgcaac gggatgtttt gtggggctgg aagcctgggg ggatgtgtcc
       61 agtgaacgtg ccattgtacg cacccgcaat atcagctgcc tgaaggacgg caaaaccatt
      121 gatatgccga ttaaggggca tgtcagcttc cggggtaaaa acggtatcaa gggcgaagtg
      181 gtgatgcgta acggcaaaat cctcggctgg gcatggggcg cgggatttgt tgacggtatc
      241 ggtcagggaa tggagcgcgc ttcccagccg gctgtcgggc tgggtgccac tgctgcttac
      301 ggggccggcg atgtccttaa aatgggtatc ggtggcggcg catcgaaagc tgcacagacg
      361 ctcagtgact actacatcaa acgtgccgaa cagtatcacc cggtgatacc gattggtgcg
      421 ggcaacgaag t
//
LOCUS       DAZFJL010000387          431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFJL010000387 DAZFJL010000000
VERSION     DAZFJL010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.390"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>431
                     /locus_tag="ACHJ8C_004883"
     CDS             <1..>431
                     /locus_tag="ACHJ8C_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO5554555.1"
                     /translation="RSLPSTKNWTHAIYFRFVIADYFISKVAKVLYLDADIICQGTIE
                     PLIKFSFPDDKVAMVVTEGQADWWEKRAHSLGVAGIAKGYFNSGFLLINTAKWAAQQV
                     SARAIAMLNEPEIIKKITHPDQDVLNMLLADKLIFADIKYNT"
BASE COUNT          130 a           78 c           89 g          134 t
ORIGIN      
        1 cgctcattgc ctagcacaaa gaactggacc catgcaatat attttcgttt tgttattgct
       61 gattacttta ttagtaaggt ggctaaagtc ctttatctgg atgcagatat catttgtcag
      121 gggactattg agccactaat taaattctca tttcctgacg ataaagtcgc aatggttgtt
      181 acagaaggac aagctgactg gtgggaaaaa cgcgcccatt cgttaggtgt tgcgggaatt
      241 gctaaaggtt actttaactc cggtttttta ttgattaata ctgccaaatg ggcggctcag
      301 caggtttctg cacgagccat tgcaatgcta aatgagccag aaataatcaa aaaaataaca
      361 catcctgatc aggatgtatt aaatatgttg ctggcagata aacttatttt cgctgatatt
      421 aaatataaca c
//
LOCUS       DAZFJL010000388          431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFJL010000388 DAZFJL010000000
VERSION     DAZFJL010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.391"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(18..110)
                     /locus_tag="ACHJ8C_004884"
                     /pseudo
     CDS             complement(18..110)
                     /locus_tag="ACHJ8C_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(366..>431)
                     /locus_tag="ACHJ8C_004885"
     CDS             complement(366..>431)
                     /locus_tag="ACHJ8C_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFO5554556.1"
                     /translation="LINVNFFSSDRTSFCECNRFP"
BASE COUNT          132 a           90 c           81 g          128 t
ORIGIN      
        1 ggcgttataa cctaaatttt taccgtccaa taatcaatat ttctattgtg attgttaacc
       61 actgcgtgca ttgaagggtg aaaaatatgt gcaattgaga agcctttcat tgatgcctgc
      121 cttgtcatca tgacaaacaa taatagatgc ttattttcct cgcagggttc tttcgcacta
      181 tcaaaaaatg ctcatttacc cggcattaca gtcaacgaag caacattcta tggcctacgc
      241 tgatgtaaag cacagcgaaa atcctccttg ttgacttcgt ctgaccttat cgcggcgacc
      301 agcaattatt cgttttgtga tcctcaacac aaaatgctat tcgttgccta tgaggggaga
      361 agaatttatg gaaaacgatt gcattcacaa aatgacgtcc tgtcagaaga gaaaaagttg
      421 acgttaatca a
//
LOCUS       DAZFJL010000389          428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFJL010000389 DAZFJL010000000
VERSION     DAZFJL010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.392"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            10..>428
                     /locus_tag="ACHJ8C_004886"
     CDS             10..>428
                     /locus_tag="ACHJ8C_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO5554557.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAEKAAEKKRRREEQKQKDKLKIQKLALKPRSYWIKQAQQAVNAFIRERDRDL
                     PCISCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQ"
BASE COUNT          150 a          100 c          108 g           70 t
ORIGIN      
        1 aagaagatga tggctaaacc agcgcgaaga cgatgtaaaa acgatgaatg tcgggaatgg
       61 tttcaccctg cattcgctaa tcagtggtgg tgctctccag agtgtggaac caagatagca
      121 ctcgaacgac gaagcaaaga acgcgaaaaa gcggaaaagg cagaaaaggc agcagagaag
      181 aaacgacgac gagaggagca gaaacagaaa gataaactta agattcaaaa actcgcctta
      241 aagccccgca gttactggat taaacaagcc caacaagccg taaacgcctt catcagagaa
      301 agagaccgcg acttaccatg tatctcgtgc ggaacgctca cgtctgctca gtgggatgcc
      361 gggcattacc ggacaactgc tgcggcacct caactccgat ttgatgaacg caatattcac
      421 aagcaatg
//
LOCUS       DAZFJL010000390          409 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFJL010000390 DAZFJL010000000
VERSION     DAZFJL010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.393"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..81
                     /locus_tag="ACHJ8C_004887"
     CDS             <1..81
                     /locus_tag="ACHJ8C_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5554558.1"
                     /translation="NTGGNKEQVPAKRQNRGSRNSTNDGG"
     gene            106..392
                     /locus_tag="ACHJ8C_004888"
                     /pseudo
     CDS             106..392
                     /locus_tag="ACHJ8C_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          127 a          101 c          111 g           70 t
ORIGIN      
        1 aataccggag ggaacaaaga gcaggtcccc gcaaaacgtc aaaaccgcgg ctcccgaaac
       61 tcaacaaatg atgggggctg aaaaccagag agaggagaaa gcaggatgag tacccgaaat
      121 atccacgtta acaccgcgtc gtacacgctt cttgttgccg ggaagaaaaa aacaccgggg
      181 aagagtggga cgtccgggaa ttcagcagcc tgactgagct gaaaaaatat cgcaaaagcc
      241 acccggaaaa gatggccttc agttatagct acgcgctcag tcagggtgtg gataagcagt
      301 tccgccatat caacattgcg gaagccgatc acttcaaaca gttcctgcgt cagattaagc
      361 gtgccggcct cgatatccgg gcgatctgct gaccggatgt gatgaatat
//
LOCUS       DAZFJL010000391          408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFJL010000391 DAZFJL010000000
VERSION     DAZFJL010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.394"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            1..>408
                     /gene="traK"
                     /locus_tag="ACHJ8C_004889"
     CDS             1..>408
                     /gene="traK"
                     /locus_tag="ACHJ8C_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFO5554559.1"
                     /translation="MAGAGTLAPTVVPMVNGGQASIAISNTSPNLFTVPGDRIIAVNS
                     LDGVLTNNEQTASGGVVVATVNKKPFTFILETERGLNLSIQAVPREGAGRTIQLVSDL
                     RGTGEEAGAWETSTPYESLLVTISQAVRGGKLPA"
BASE COUNT           87 a          117 c          120 g           84 t
ORIGIN      
        1 atggccggag ccggtacgct ggcccccact gtggtgccaa tggtgaacgg tgggcaggcc
       61 agtattgcca tcagcaatac cagcccgaat ctgtttaccg ttcccggtga ccggattatc
      121 gccgtgaaca gtctggatgg tgtactgacc aataatgagc agaccgcctc cggcggtgtg
      181 gtggtggcca ccgtcaacaa aaagcccttt acgttcattc tggaaacaga acgtggtctt
      241 aacctttcca ttcaggccgt tccccgtgaa ggtgcggggc gtaccattca gctggtcagt
      301 gacctgcgcg gaaccggaga agaagccggt gcgtgggaaa cgtccacgcc ttacgaatcc
      361 ctgcttgtga ccatcagcca ggccgtccgt ggcggaaaat tacctgca
//
LOCUS       DAZFJL010000392          408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFJL010000392 DAZFJL010000000
VERSION     DAZFJL010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.395"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>408
                     /locus_tag="ACHJ8C_004890"
     CDS             <1..>408
                     /locus_tag="ACHJ8C_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO5554560.1"
                     /translation="EGYGSVFGVKDSHDDVVMSGAFAASLRAWSDRKALPALLWQHRM
                     DEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHAHMKAGSLTGLSIGYVLKDWEYD
                     RSKEAFLLKEIDLWEVSLVTFPSNDEARISDVK"
BASE COUNT           97 a           98 c          124 g           89 t
ORIGIN      
        1 tgaagggtat ggctccgtct ttggtgtaaa ggacagccac gatgatgtgg tgatgtccgg
       61 ggcatttgct gcttccctgc gggcgtggag tgacagaaaa gcgttacctg cgctgctctg
      121 gcagcaccgc atggatgaac ccatcggtgt ttacaccgaa atgaaggaag acgatgtcgg
      181 gctttacgtc aggggacggt tgcttattga tgatgatccc ctcgcaaaac gcgcacatgc
      241 acacatgaag gccggttcgt taaccggcct ttctattggg tacgtcctga aagactggga
      301 atacgaccgg agcaaagaag cctttctgct gaaagaaatc gacctctggg aagtcagcct
      361 ggtgacgttc ccgtctaacg acgaggcgcg gatcagcgac gtcaagaa
//
LOCUS       DAZFJL010000393          407 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFJL010000393 DAZFJL010000000
VERSION     DAZFJL010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.396"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..>407)
                     /locus_tag="ACHJ8C_004891"
     CDS             complement(<1..>407)
                     /locus_tag="ACHJ8C_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO5554561.1"
                     /translation="VWCGDITYIWAGNCWCYLAVVMDLFARRVIGWSLSAHADTALIS
                     DALRMAYETRGQPHDVMFHSDQGSQYTGLKYQQILWRYRIKQSVSRRGNCWDNSPMER
                     FFRSLKTEWVPANGYVDKDEARQQINDYILNYY"
BASE COUNT           97 a          113 c           88 g          109 t
ORIGIN      
        1 ttgtagtagt tcaatatgta atcattaatt tgctgccggg cctcgtcctt gtcgacgtaa
       61 ccattcgctg gcacccattc tgttttcaga ctgcggaaga agcgttccat tgggctatta
      121 tcccagcagt ttccccgccg actgacactt tgctttattc tgtaacgcca gagaatttgt
      181 tgatatttca ggcctgtata ctggcttccc tggtcgctat ggaacataac atcatgtggt
      241 tgaccacgcg tctcataggc catccgcagg gcatcgctta tcagtgcagt atcggcatgt
      301 gcggacagac tccagccgat aaccctgcgg gcaaaaagat ccatcacaac cgccagatag
      361 caccagcaat ttccggccca gatataggta atatctccac accatac
//
LOCUS       DAZFJL010000394          395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFJL010000394 DAZFJL010000000
VERSION     DAZFJL010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.397"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            135..>395
                     /gene="grlR"
                     /locus_tag="ACHJ8C_004892"
     CDS             135..>395
                     /gene="grlR"
                     /locus_tag="ACHJ8C_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE GrlA-binding
                     negative regulator GrlR"
                     /protein_id="HFO5554562.1"
                     /translation="MIMKDGIYSIIFISNEDSCGEGILIKNGNMITGGDIASVYQGVL
                     SEEEDIILHVHRYNYEIPSVLNIEQDYQLVIPKKVLSNDNNLT"
BASE COUNT          137 a           53 c           84 g          121 t
ORIGIN      
        1 ggttgaaaca tgagtatatt taatgatata ttacattgca atctggagaa aaagaaaggt
       61 ctccattatt cttgatattg cttatggata gaacaaattg aaaggagtga ggttagtatg
      121 aaactgagtg agttatgatt atgaaggatg gcatctatag cattatattt attagcaacg
      181 aagactcctg tggggaaggt atactgatta aaaatggaaa tatgatcact ggcggcgata
      241 ttgcttctgt gtatcagggg gttctctctg aagaggagga catcatactt catgtccatc
      301 gatataatta cgaaattccc tcggtgctaa acattgaaca agattatcaa ttagttatcc
      361 ctaaaaaagt actgagtaat gataataatc tcaca
//
LOCUS       DAZFJL010000395          393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFJL010000395 DAZFJL010000000
VERSION     DAZFJL010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.398"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..356)
                     /locus_tag="ACHJ8C_004893"
     CDS             complement(<1..356)
                     /locus_tag="ACHJ8C_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5554563.1"
                     /translation="MSSNFERSQQTKVMISSAPVTAETLSSASFLELSCTIKEVQFTA
                     GQKQDIDVTTLCSVEQENINGLGAASEISMSGNFYLNAAQNALRSAYDNDTTYGFKVI
                     FPSGNGFTFMAEVRQHT"
BASE COUNT          113 a           98 c           88 g           94 t
ORIGIN      
        1 gtatgctgac gcacctctgc cataaaggta aatccgttgc ctgacggaaa aataacttta
       61 aagccatacg tggtgtcatt gtcataggca ctgcgcaacg cgttctgggc agcattcaga
      121 taaaagttgc ctgacatgga aatctctgac gcggcaccaa gaccgttaat attttcctgc
      181 tcaacagaac acagcgtggt gacatcaata tcctgtttct gcccggcggt aaactgaacc
      241 tctttaatcg tacagctcag ttcaagaaaa ctggcagaac tcagcgtttc tgccgttacc
      301 ggtgcagacg agatcatgac tttggtctgc tgagaacgtt caaaattaga ggacatactc
      361 gtctcctgaa aataaaaaaa cccgccagcg gcg
//
LOCUS       DAZFJL010000396          392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZFJL010000396 DAZFJL010000000
VERSION     DAZFJL010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.399"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..371)
                     /gene="ler"
                     /locus_tag="ACHJ8C_004894"
     CDS             complement(<1..371)
                     /gene="ler"
                     /locus_tag="ACHJ8C_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HFO5554564.1"
                     /translation="MNMETNSHTTSPYIQLIEQIAVLQQEAKRLREQEVQSVIESIQK
                     QITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNG
                     MKKEDFLVKDTEEEIIPLKNI"
BASE COUNT           92 a           93 c           69 g          138 t
ORIGIN      
        1 taaatatttt tcagcggtat tatttcttct tcagtgtcct tcacaagaaa atcttctttc
       61 ttcattccat tcaacagtgc ttctttaagc cagcgtggct gtcggcctac gcccgaccag
      121 gtctgccctt cttcattgcg gtagtaaaca cctttcgatg agttccggcg agcgagtcca
      181 tcatcaggca cattagtata tcccagctct tgtaaggtta tattgtaata agtaatctgc
      241 ttctgaatcg actcaattac actttgaact tcctgctctc gcagtcgctt tgcttcctgc
      301 tgtagaactg caatttgctc tataagctga atgtatggac ttgttgtatg tgaattagtt
      361 tccatattca taataaataa tctccgcatg ct
//
LOCUS       DAZFJL010000397          388 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZFJL010000397 DAZFJL010000000
VERSION     DAZFJL010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.400"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>388
                     /locus_tag="ACHJ8C_004895"
     CDS             <1..>388
                     /locus_tag="ACHJ8C_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5554565.1"
                     /translation="VQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSK
                     QGVYYAKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPM
                     NMTTKPSDGSKAGKQKDNANADETTS"
BASE COUNT          107 a          103 c          100 g           78 t
ORIGIN      
        1 tggtgcagaa caccgatcgc gccaccttca acaatatcga agagctgggg ctcggattta
       61 tcaactattc actggtgccg tatctgaccc gcatcgaaca gcggatcaac accggactgg
      121 tacgaaaaag taagcagggc gtttattacg ccaaatttaa cgccggggcg ttactgcgcg
      181 gggatatgaa gtcccgtttt gaagcctacg ccaccgggat taactgggga atttactctc
      241 ccaatgactg ccgcgacctg gaagatatga atccgcgtcc cggtggtgat gtctatctca
      301 caccgatgaa catgaccacg aaaccctccg atggcagtaa agccggtaag cagaaggata
      361 acgccaatgc agacgaaaca acgtcttg
//
LOCUS       DAZFJL010000398          383 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZFJL010000398 DAZFJL010000000
VERSION     DAZFJL010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.401"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            50..>383
                     /locus_tag="ACHJ8C_004896"
     CDS             50..>383
                     /locus_tag="ACHJ8C_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO5554566.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     STIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENTSQ
                     LHEVWQKGA"
BASE COUNT          120 a           83 c           89 g           91 t
ORIGIN      
        1 tacaccacct ttgaaaacaa tccgctatgc tttgaaaaag gagaaagaaa tgatgaaaaa
       61 gtttattgcc cccttgttgg ctttactggt tagcggatgt cagattgatc cttatactca
      121 cgcgccaacc ttgaccagca ccgactggta tgatgtcggt atggaagatg cgatatcggg
      181 cagcaccata aaagatgacg atgcatttag cgattcacag gcggatcgcg gtctatacct
      241 taaaggatat gccgaaggac aaaagaaaac ttgccagacc gattttactt atgcccgagg
      301 actttccggt aaaagctttc ctgccagctg caacaatgtt gaaaatacaa gccaactgca
      361 tgaagtatgg caaaaagggg ctg
//
LOCUS       DAZFJL010000399          380 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZFJL010000399 DAZFJL010000000
VERSION     DAZFJL010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.402"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..371
                     /locus_tag="ACHJ8C_004897"
     CDS             <1..371
                     /locus_tag="ACHJ8C_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5554567.1"
                     /translation="HEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYSDDGLAETDN
                     NTAERALRAVCLGKKNYVFFGSDHGGERGALLYGLIGTCRLNGIDPEAYLRHILSVLP
                     EWPSNRVDELLPWNVVLTNK"
BASE COUNT           97 a           88 c          111 g           84 t
ORIGIN      
        1 tgcatgaatg gatggtggag aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag
       61 cgttcagcta tgtactgaat cagtgggacg ccctctgtta ttacagtgat gacggactgg
      121 cggagacgga caataacaca gcggaaagag cgcttcgtgc agtctgtctt ggaaagaaaa
      181 attacgtgtt cttcggtagc gatcacggcg gcgagcgtgg tgcactgctg tacgggctga
      241 tcggcacctg ccgtctgaac ggtatcgatc cggaagcgta tctgcgccat attctgagcg
      301 tactgccgga atggccctcc aaccgtgttg acgaactcct gccatggaac gtagttctca
      361 ccaataaata agcgtcaata
//
LOCUS       DAZFJL010000400          377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZFJL010000400 DAZFJL010000000
VERSION     DAZFJL010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.403"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            122..>377
                     /locus_tag="ACHJ8C_004898"
     CDS             122..>377
                     /locus_tag="ACHJ8C_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="catalyzes the formation of UDP-glucuronate from
                     UDP-glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO5554568.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDY"
BASE COUNT          118 a           72 c           71 g          116 t
ORIGIN      
        1 gaaaatctga attgtttttc gttgcgttga ccatctgaca aggtagcgtt aaaactttta
       61 gctcttatca agatgctaaa aacatcatga ttcacagtta agttaattct gagagcatga
      121 aatgaaaatc accatttccg gtactggcta tgtaggctta tcaaacgggc ttctaatcgc
      181 acaaaatcat gaggttgtgg cattagatat tttaccgtca cgcgttgcta tgttgaatga
      241 tcggatatct cctattgtcg ataaggaaat tcagcagttt ttgcaatcag ataaaataca
      301 ctttaatgcc acattagata aaaatgaagc ctaccgggat gctgattatg tcatcatcgc
      361 cactccaacc gactatg
//
LOCUS       DAZFJL010000401          371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZFJL010000401 DAZFJL010000000
VERSION     DAZFJL010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.404"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..243)
                     /locus_tag="ACHJ8C_004899"
     CDS             complement(<1..243)
                     /locus_tag="ACHJ8C_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M85 family metallopeptidase"
                     /protein_id="HFO5554569.1"
                     /translation="MICRVLDINMKIPSLQSNFNFSAPAGYSAPIAPNRAENAYADYV
                     LDIGKRIPLSAADLSNVYESVIRAVHDSRSRLIDQHT"
     gene            complement(277..>371)
                     /locus_tag="ACHJ8C_004900"
     CDS             complement(277..>371)
                     /locus_tag="ACHJ8C_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554570.1"
                     /translation="LHNYNHFCDFIEFNHPNIIMNTSQYTCSSW"
BASE COUNT          116 a           65 c           76 g          114 t
ORIGIN      
        1 tgtatgctga tcgataagcc tgctacggct gtcatggacg gcgcgtatta cactttcgta
       61 tacgttgctt aaatctgctg cggaaagtgg tattcgctta cctatatcca aaacgtaatc
      121 cgcataggca ttttcagcac gattaggagc aatgggagca gagtatcctg ccggggcgga
      181 aaagttgaag ttggactgta atgagggaat tttcatgttt atatctaata ccctgcaaat
      241 catttattac aacaaaataa actatatcaa attcatttac catgaactgc atgtatactg
      301 acttgtgttc atgattatat tagggtggtt aaactcaata aagtcacaaa aatgattata
      361 attatgcaat g
//
LOCUS       DAZFJL010000402          360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZFJL010000402 DAZFJL010000000
VERSION     DAZFJL010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.405"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>360
                     /locus_tag="ACHJ8C_004901"
     CDS             <1..>360
                     /locus_tag="ACHJ8C_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO5554571.1"
                     /translation="TLSRQYRDEVGALSSQLALPRNTQVDRVIYGINCTDLSLADRGR
                     AAGLVSQFGSFSFTVAHAFCRLVYQLGVKNSLMALESAHRFAFFNQDGDPYEFSLFCT
                     DEQLAEVADSVVRDCLVS"
BASE COUNT           56 a           94 c           99 g          111 t
ORIGIN      
        1 acgctttccc gtcagtatcg tgatgaggtc ggggcgctct cttcacagct tgccctgcca
       61 cgtaacacgc aggttgaccg tgtgatttac ggaattaact gcacggattt atcccttgct
      121 gacaggggca gggccgccgg actggtatca cagtttggct cgttttcctt caccgtggcg
      181 cacgctttct gccgtctggt ttatcagctc ggtgtgaaaa attcgctgat ggcgcttgag
      241 tctgcgcacc gtttcgcttt ctttaatcag gacggggacc cttatgaatt ctcgcttttc
      301 tgcactgatg agcagcttgc tgaggtggct gattcggttg ttcgtgactg ccttgtttct
//
LOCUS       DAZFJL010000403          349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZFJL010000403 DAZFJL010000000
VERSION     DAZFJL010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.406"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..304
                     /locus_tag="ACHJ8C_004902"
     CDS             <1..304
                     /locus_tag="ACHJ8C_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HFO5554572.1"
                     /translation="SHFRKVINPKHFISPVYVKPKMSNKRIIAQSGAFLLYPHHPEQT
                     KNHQGNDLDIHVETLIVQARAKAKIRGQLAKLGIHAGSIFPEMDKASEYILAQYNK"
BASE COUNT          131 a           62 c           57 g           99 t
ORIGIN      
        1 gtctcatttt agaaaagtta ttaaccccaa gcattttatt agtcctgttt atgtcaaacc
       61 caaaatgagt aataaaagaa ttatcgctca atcaggagca tttttacttt atcctcatca
      121 tccagaacaa acaaaaaacc atcagggtaa cgatctagat attcatgtgg aaacattgat
      181 tgtacaagca agagctaaag caaaaattcg tgggcaactg gctaagttgg gaattcatgc
      241 tggttcaatt ttcccggaaa tggataaagc atcagaatac atacttgctc aatataataa
      301 ataaacttca attcatgtct gcgtgaaaaa ctataaactg caaagaggt
//
LOCUS       DAZFJL010000404          333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZFJL010000404 DAZFJL010000000
VERSION     DAZFJL010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.407"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            117..>333
                     /locus_tag="ACHJ8C_004903"
     CDS             117..>333
                     /locus_tag="ACHJ8C_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO5554573.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGLLLAQKALHKTSDTAFCLSCHSM
                     SKPFEEYQGTVHFSNQKGIRAECADCHI"
BASE COUNT           87 a           57 c           81 g          108 t
ORIGIN      
        1 catattcttt atcgattatt tcaggcagtg ttgtgccgaa ttaagcaagt agtcaattcg
       61 ttgtgtattt atttatacaa tggtatcggt gtcctggaat ttaattagag gaatctatgc
      121 gagggaaaaa acgcattggg ttattgtttt tgctgatagc ggttgtggtt ggtggcgggt
      181 tattactggc gcaaaaagcc ttacataaaa cgtcggatac cgcattttgc ctttcctgcc
      241 actcgatgag taaacctttt gaggaatatc agggaactgt ccacttttca aaccagaaag
      301 ggatacgtgc ggaatgtgcc gattgccata ttc
//
LOCUS       DAZFJL010000405          324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZFJL010000405 DAZFJL010000000
VERSION     DAZFJL010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.408"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..221
                     /locus_tag="ACHJ8C_004904"
     CDS             <1..221
                     /locus_tag="ACHJ8C_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFO5554574.1"
                     /translation="AGLSSVADAVWTGNHLKMIRFAVENKTLSALNIRESDFWQPGTR
                     AVMFSQPAGQLLAGARMDVYVIRDGEGN"
     gene            221..>324
                     /locus_tag="ACHJ8C_004905"
     CDS             221..>324
                     /locus_tag="ACHJ8C_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HFO5554575.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGALYLS"
BASE COUNT           64 a           76 c          109 g           75 t
ORIGIN      
        1 cggcggggct gtcttcagtg gcagatgctg tatggacggg gaatcacctg aagatgatcc
       61 gctttgccgt ggaaaataaa acgctgtctg ccctgaatat ccgggaaagt gacttctggc
      121 agccgggaac ccgtgccgtg atgttcagcc agcctgccgg ccagttactg gcaggtgcgc
      181 gcatggatgt gtatgtcatc cgtgacgggg agggcaactg atggccagta tcaataccat
      241 tgtgaaacgc aagcagtacc tgtggctggg gattgtggtt gtcggtgcag cctccgcgat
      301 tggtggggca ctgtatctgt ctga
//
LOCUS       DAZFJL010000406          323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZFJL010000406 DAZFJL010000000
VERSION     DAZFJL010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.409"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..145)
                     /locus_tag="ACHJ8C_004906"
     CDS             complement(<1..145)
                     /locus_tag="ACHJ8C_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFO5554576.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVL"
     gene            complement(135..>323)
                     /locus_tag="ACHJ8C_004907"
     CDS             complement(135..>323)
                     /locus_tag="ACHJ8C_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HFO5554577.1"
                     /translation="EMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQMLKQLGN
                     LNPQQFMSGSQGGGNDGK"
BASE COUNT           74 a           94 c           83 g           72 t
ORIGIN      
        1 ataaaacaaa cagagccatc agggccattg tggccagtgg cagaatgacg gtgtacttca
       61 gacaccagaa cagtcctcgc agaacccggg cgacaacata ccgggcggca tggcacgcct
      121 gacgggacga catattattt gccatcgttg cctcctccct ggctgccgga cataaactgc
      181 tgcggattca ggttgcccag ctgcttcagc atctggtcag tattaaagcc gttaagatga
      241 ctttcagctg atggcggaac aggaaccgga ctttccggat tatcttcttc cgctctgttc
      301 tgcgtgcgct ccagcgccat ctc
//
LOCUS       DAZFJL010000407          318 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZFJL010000407 DAZFJL010000000
VERSION     DAZFJL010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.410"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>318
                     /gene="rfaJ"
                     /locus_tag="ACHJ8C_004908"
     CDS             <1..>318
                     /gene="rfaJ"
                     /locus_tag="ACHJ8C_004908"
                     /EC_number="2.4.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase RfaJ"
                     /protein_id="HFO5554578.1"
                     /translation="LYRINTDKLQCLPCTQVWSRAMYFRLFAFQLLGLTLDRLLYLDA
                     DVVCKGDISQLLHLDLNGAVAAVVKDVDPMQEKAASRLSDPELLGQYFNSGVVYLDLK
                     KWAN"
BASE COUNT           83 a           52 c           71 g          112 t
ORIGIN      
        1 ttatatagaa ttaacactga taagctccag tgcttgcctt gtacacaggt ctggtcaaga
       61 gcaatgtact tccgtttatt tgcatttcaa ttattgggtt taacacttga tcgtttgctt
      121 tatcttgatg cagatgttgt ttgtaaaggc gatattagcc aactattgca tctggattta
      181 aacggagcgg ttgctgctgt tgttaaagat gttgacccaa tgcaggaaaa ggcggcatcc
      241 aggttgtctg atcctgaatt acttgggcag tactttaatt ctggtgtcgt ttatttagat
      301 ttaaaaaaat gggctaac
//
LOCUS       DAZFJL010000408          316 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZFJL010000408 DAZFJL010000000
VERSION     DAZFJL010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.411"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..144)
                     /locus_tag="ACHJ8C_004909"
     CDS             complement(<1..144)
                     /locus_tag="ACHJ8C_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5554579.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQIIEQINIALDLKGSGNFSAWVIEA
                     CRRR"
BASE COUNT           77 a           54 c           65 g          120 t
ORIGIN      
        1 tcttcttctg caggcttcaa taacccacgc tgaaaaatta cctgaacctt tcaggtcaag
       61 agcgatgtta atttgttcaa ttatctggtt tggaaatcgg atgttgcggg ttgttgttct
      121 gcgggttctg ttctttgatg acataatgtt gccccgtatt cagtgttgct gatttgtatt
      181 atctgaagtt gcttttacgc taatttgatg cagatcaatt aatacgatac ctgcgtaata
      241 attgattatt tctcgtggtt tgatggcgta cacacatgtc gtgataaacc tcatgtagat
      301 gataattatt atcatt
//
LOCUS       DAZFJL010000409          311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZFJL010000409 DAZFJL010000000
VERSION     DAZFJL010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.412"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(216..>311)
                     /locus_tag="ACHJ8C_004910"
     CDS             complement(216..>311)
                     /locus_tag="ACHJ8C_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554580.1"
                     /translation="SLIDSAISKFQHQIPVNGSVNRELLYEDYTD"
BASE COUNT          102 a           46 c           63 g          100 t
ORIGIN      
        1 agtcgaattt cactgcaatt catctctgct taatgtaaaa ataatgttga aaataacaaa
       61 tggttaatga ttcatgcgaa ttatgacctt agttagctct tttgattttt cgtaaaaaag
      121 agaggtacat gaaggtgcta ccagtatgag taacgttaat caaaggtttt attacctctg
      181 gcaataacgc aggccatttt cgcgactggg tggatttaat cagtataatc ctcatacaat
      241 agttcacgat taactgaacc gttaacgggg atttggtgtt gaaatttaga aatggcggag
      301 tcaattaatg a
//
LOCUS       DAZFJL010000410          307 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZFJL010000410 DAZFJL010000000
VERSION     DAZFJL010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.413"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            51..>307
                     /locus_tag="ACHJ8C_004911"
     CDS             51..>307
                     /locus_tag="ACHJ8C_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020314677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554581.1"
                     /translation="MENHAKFVATEILNQLGGNRFIAMTGAKNFACFDENGECGLCFR
                     LPSNFAMKGINLVKIKLTFSDTYLVTFSRVRGATVKEISKF"
BASE COUNT           91 a           58 c           67 g           91 t
ORIGIN      
        1 gaggggatgg tccccaatca ccaaagccgc tcagcggcag gagcttaaaa atggaaaacc
       61 acgccaaatt tgttgctact gaaattctta atcagcttgg tggaaaccgc tttattgcta
      121 tgacaggcgc aaagaacttt gcctgttttg atgaaaatgg tgagtgtggt ctttgctttc
      181 gtttgccttc aaattttgca atgaagggaa ttaatttggt taaaatcaaa ttaaccttct
      241 ctgatacata cctggtgaca ttttcccgtg tccgtggagc tacagtaaaa gaaatatcaa
      301 aatttga
//
LOCUS       DAZFJL010000411          301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZFJL010000411 DAZFJL010000000
VERSION     DAZFJL010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.414"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>301
                     /locus_tag="ACHJ8C_004912"
     CDS             <1..>301
                     /locus_tag="ACHJ8C_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554582.1"
                     /translation="FIHATVINDEELKPGGTHYNPEAKPKYAIVIAYPYEDKKLKVRR
                     EEHERFSCTEEDFKFFNSCPDLKGKPVYLTVDVNSWATGSERHGVWYRFISGSMKR"
BASE COUNT           81 a           59 c           68 g           93 t
ORIGIN      
        1 attttattca cgccactgtt ataaatgatg aggagttaaa gcccggtggt actcattata
       61 accctgaggc taaacccaaa tatgccattg ttattgccta tccgtatgag gacaaaaaat
      121 taaaagtccg tcgtgaggag catgagcgct tttcctgtac tgaggaggat tttaaatttt
      181 ttaattcatg tcctgacctg aagggtaaac ctgtttatct cacggtggat gtgaattcat
      241 gggcaacagg ctctgaacgt catggcgtct ggtatcgttt tatttccggc tccatgaaac
      301 g
//
LOCUS       DAZFJL010000412          299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZFJL010000412 DAZFJL010000000
VERSION     DAZFJL010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.415"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..>299)
                     /locus_tag="ACHJ8C_004913"
     rRNA            complement(<1..>299)
                     /locus_tag="ACHJ8C_004913"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           69 a           83 c           72 g           75 t
ORIGIN      
        1 aaacattaaa gggtggtatt tcaaggtcgg ctccatgcag actggcgtcc acacttcaaa
       61 gcctcccacc tatcctacac atcaaggctc aatgttcagt gtcaagctat agtaaaggtt
      121 cacggggtct ttccgtcttg ccgcgggtac actgcatctt cacagcgagt tcaatttcac
      181 tgagtctcgg gtggagacag cctggccatc attacgccat tcgtgcaggt cggaacttac
      241 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt taccggggc
//
LOCUS       DAZFJL010000413          295 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZFJL010000413 DAZFJL010000000
VERSION     DAZFJL010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.416"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..260
                     /gene="etgA"
                     /locus_tag="ACHJ8C_004914"
     CDS             <1..260
                     /gene="etgA"
                     /locus_tag="ACHJ8C_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE muramidase EtgA"
                     /protein_id="HFO5554583.1"
                     /translation="SSHLDLLSKFNISEDDLLNDACINISVAGYILASNIKSRGNTWD
                     AVGAYNAGYFNTPNAVELRRQYAMKIYKTYNKLKNNEQIID"
BASE COUNT          116 a           41 c           48 g           90 t
ORIGIN      
        1 attcatctca tttagattta ttaagcaaat ttaatatatc tgaagatgat ttattgaatg
       61 atgcctgtat aaacatatct gttgctggtt atatcttagc ctcaaacata aaatccagag
      121 ggaatacatg ggatgcagtt ggtgcgtata atgcaggtta ctttaataca ccaaatgcag
      181 tagaactcag aaggcaatac gcaatgaaaa tatataaaac atataataaa ttaaagaata
      241 atgagcaaat tatcgattaa taatcaaggt ctatcgcaat acgaatcaat tgtgc
//
LOCUS       DAZFJL010000414          287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZFJL010000414 DAZFJL010000000
VERSION     DAZFJL010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.417"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..>287)
                     /locus_tag="ACHJ8C_004915"
     CDS             complement(<1..>287)
                     /locus_tag="ACHJ8C_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO5554584.1"
                     /translation="LLTIPIPEIETLPFNEFIYTVALTKYSDFVISVDTALVHIAAAY
                     HKPTLAFYPNSRTPEYPSHLIWSPNNHKSIQIVSPTFTVKDIDTETLTNSV"
BASE COUNT           97 a           36 c           61 g           93 t
ORIGIN      
        1 ttactgaatt agttaaagtt tcagtatcga tatctttcac cgtaaatgtt ggggatacta
       61 tttgtattga tttatgatta tttggggacc aaattaaatg tgagggatat tccggagtac
      121 gtgaattagg ataaaatgca agcgttggtt tatgataagc cgcagcgata tgaacaagag
      181 ctgtatcaac agaaatgaca aaatcactat acttggtcaa cgcaacggta taaataaact
      241 cattaaatgg tagtgtttcg atttcaggta ttggtattgt caataaa
//
LOCUS       DAZFJL010000415          280 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZFJL010000415 DAZFJL010000000
VERSION     DAZFJL010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.418"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>280
                     /locus_tag="ACHJ8C_004916"
     CDS             <1..>280
                     /locus_tag="ACHJ8C_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5554585.1"
                     /translation="NDGWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLAGAKS
                     GGVFQAVLGAALIAVAWWNPAGWLGAAALSGMYAAGASMILGGVAQMLA"
BASE COUNT           45 a           66 c          107 g           62 t
ORIGIN      
        1 tgaatgacgg ctggtatcag gtgcgcattg ccgggcgtga tgcaggtgaa accgaattat
       61 ctgcccgtct taatgagccg ctggcaaatg gtgccgtgat ccacatcgta ccgcgcctgg
      121 cgggggctaa aagtggcggc gtatttcagg ctgtgctggg ggcggcgctg attgcggtgg
      181 catggtggaa ccctgcaggc tggctgggtg ctgcggcttt atcgggcatg tatgcggcag
      241 gagccagtat gatcctgggc ggtgtggccc agatgctggc
//
LOCUS       DAZFJL010000416          278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZFJL010000416 DAZFJL010000000
VERSION     DAZFJL010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.419"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>278
                     /locus_tag="ACHJ8C_004917"
     CDS             <1..>278
                     /locus_tag="ACHJ8C_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5554586.1"
                     /translation="EEAARHAEEAKKNAGEAETSARNAGISASQAEESAANADTSAGD
                     ASESARQAAESAAAAKQSEEASSSSASEAAQKASESLQSAADAELSKN"
BASE COUNT           79 a           69 c           94 g           36 t
ORIGIN      
        1 ggaagaagcg gcgcgtcacg ctgaggaggc gaagaagaat gccggagagg cggagacgtc
       61 cgcgaggaat gccggcatat cagccagtca ggcagaagag agcgctgcaa atgctgacac
      121 ttcagcaggg gatgcatcgg agtcagcccg gcaggcggca gaaagtgcag ccgctgcaaa
      181 gcagtcagag gaagcgtcct cgtcctcggc ctctgaggcc gctcaaaaag ccagtgagtc
      241 attacaaagt gcagcagatg ctgagttgtc aaaaaaca
//
LOCUS       DAZFJL010000417          276 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZFJL010000417 DAZFJL010000000
VERSION     DAZFJL010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.420"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>276
                     /gene="traA"
                     /locus_tag="ACHJ8C_004918"
     CDS             <1..>276
                     /gene="traA"
                     /locus_tag="ACHJ8C_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFO5554587.1"
                     /translation="RLNMLRLARAVIPAAVLMMFFPQLAMAAGSSGQDLMASGNTTVK
                     ATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFLAGFAIISVFIAVG"
BASE COUNT           48 a           64 c           82 g           82 t
ORIGIN      
        1 tcgtctgaat atgcttcgcc tggctcgcgc tgtgatcccg gctgctgttc tgatgatgtt
       61 cttcccgcag ctggcgatgg ccgctggcag cagtggtcag gacctgatgg caagtggtaa
      121 caccacggtt aaggcgacct tcggtaagga ctccagtgtt gttaaatggg ttgttctggc
      181 tgaagttctg gtcggtgcgg tcatgtacat gatgaccaaa aacgtcaagt tcctggccgg
      241 ttttgccatc atctctgtat ttattgctgt gggtat
//
LOCUS       DAZFJL010000418          275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZFJL010000418 DAZFJL010000000
VERSION     DAZFJL010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.421"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>275
                     /locus_tag="ACHJ8C_004919"
     CDS             <1..>275
                     /locus_tag="ACHJ8C_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO5554588.1"
                     /translation="FGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAP
                     FVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV"
BASE COUNT           49 a           84 c           83 g           59 t
ORIGIN      
        1 ttcggcgtga cctacaccgg taactatgag ttcccgcagc cgctgcacga tgcgctggat
       61 aaattccagg ccgacaccgg tatcgacatc gacatgcaca tcgacgccgc cagcggtggc
      121 ttcctggcac cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa
      181 tcgatcagtg cttcaggcca taaattcggt ctggctccgc tgggctgcgg ctgggttatc
      241 tggcgtgatg aagaagcgct gccgcaggaa ctggt
//
LOCUS       DAZFJL010000419          273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZFJL010000419 DAZFJL010000000
VERSION     DAZFJL010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.422"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..147)
                     /locus_tag="ACHJ8C_004920"
     CDS             complement(<1..147)
                     /locus_tag="ACHJ8C_004920"
                     /inference="COORDINATES: protein motif:HMM:NF017333.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="HFO5554589.1"
                     /translation="MGGRVIKGALRSAPSRKVLLTLDEETNKLLLGARERSGRTKTNE
                     ALVRL"
BASE COUNT           90 a           66 c           35 g           82 t
ORIGIN      
        1 caacctcacc aatgcttcgt ttgttttcgt tcttccacta cgctctctgg ctccaagtaa
       61 taatttattc gtttcctcat caagggtaag taaaaccttt ctacttggcg cagatcttaa
      121 agctccttta atcaccctcc ctcccataaa taaaagagat ttagtcaaaa tattcacata
      181 agacgcacac aaaaataaac aacaaaatag taaattacgt taacaaaaac cggcactttt
      241 agtgccggga atgcttacat ttctttacag tga
//
LOCUS       DAZFJL010000420          256 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZFJL010000420 DAZFJL010000000
VERSION     DAZFJL010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.423"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
BASE COUNT           75 a           74 c           53 g           54 t
ORIGIN      
        1 aatgctggag aaccggcgaa agccgtgccg gaaatccaga aaaatgctat aattaatact
       61 ccagaaaata ctctcatata actcacctgc aatgcagcta ttcaggaaca ccccgcttag
      121 cagggcgatg gcccactgcg tgaaccatca cccccatcaa gggtatcttt tccgtgcgtc
      181 gtcaatcact aaatcggaac ccactggcag gccagcggga ccccacgatt tagtatttga
      241 cgagttaatc ccttca
//
LOCUS       DAZFJL010000421          254 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZFJL010000421 DAZFJL010000000
VERSION     DAZFJL010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.424"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..>254)
                     /locus_tag="ACHJ8C_004921"
     CDS             complement(<1..>254)
                     /locus_tag="ACHJ8C_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554590.1"
                     /translation="TLSGVLNGIAGNIDTVATAAGALVAVGVARYFGNMASSAGSATA
                     GLITAARNEVALAEAQLRGTQIATARARAAVYRAQQAVVAA"
BASE COUNT           59 a           82 c           65 g           48 t
ORIGIN      
        1 gcagcaacaa ccgcctgttg cgcacgataa accgccgcac gcgccctggc ggttgctatc
       61 tgtgtccccc gaagttgcgc ttcagcaaga gccacttcgt ttctggctgc agtaattaat
      121 ccggcagttg cagatccagc agacgacgcc atattgccaa aatatcgggc taccccgacg
      181 gcaaccagag caccggcagc ggttgccacg gtgtcaatat tgcctgcaat accattcagc
      241 acaccggaga gcgt
//
LOCUS       DAZFJL010000422          245 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZFJL010000422 DAZFJL010000000
VERSION     DAZFJL010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.425"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>245
                     /locus_tag="ACHJ8C_004922"
     CDS             <1..>245
                     /locus_tag="ACHJ8C_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HFO5554591.1"
                     /translation="VRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDDQRRIEKL
                     GQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPE"
BASE COUNT           61 a           68 c           79 g           37 t
ORIGIN      
        1 aggtgcgtca gcatgccacc acagagatgc aggtgacggc agcgcagatg cagaagcagt
       61 atgaggaaat ccgtcgtgag ctggatgttc tgaacaaaca gcgcggtgat gaccagcgtc
      121 gtattgaaaa gctgggacag gacaatgccg ccctggcaga gcaggtaaaa gccctgggtg
      181 ctaatcccgt cacggcgacc ggtgagcctg taccgcagat gcctgcctca ccgcccggcc
      241 cggaa
//
LOCUS       DAZFJL010000423          245 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZFJL010000423 DAZFJL010000000
VERSION     DAZFJL010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.426"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>245
                     /locus_tag="ACHJ8C_004923"
     CDS             <1..>245
                     /locus_tag="ACHJ8C_004923"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554592.1"
                     /translation="LPPDEPPEVLPPDEPPEVLPPDEPPELLPPDEPPELLPPDEPPE
                     LSPPDDPPETPPPDKFPELSPPSVLPLSPLSSVSPLSS"
BASE COUNT           57 a           87 c           52 g           49 t
ORIGIN      
        1 ctgccaccgg atgaaccgcc ggaagtgctg ccaccggacg aaccgccgga agtgctgcca
       61 ccggatgaac cgccggaact gctgccgccg gatgaaccgc cggaactgct accaccggat
      121 gaaccgccgg aattatcacc acctgatgac cctcctgaaa cgccaccacc ggataaattc
      181 cccgaattat caccaccgtc agtattaccg ttatctcctt tatcttcagt atctccttta
      241 tcttc
//
LOCUS       DAZFJL010000424          244 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZFJL010000424 DAZFJL010000000
VERSION     DAZFJL010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.427"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>244
                     /locus_tag="ACHJ8C_004924"
     CDS             <1..>244
                     /locus_tag="ACHJ8C_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5554593.1"
                     /translation="LTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQM
                     ELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTL"
BASE COUNT           64 a           75 c           63 g           42 t
ORIGIN      
        1 ctgaccctgc tggccatcga tggtgtgttc tggcgcacac cggatacacc agagaacgat
       61 gcagccttcc cccgccagac acatgccggg aacccggcgc tctacccgca ggtcaaaatg
      121 gtctgccaga tggaactgac cagccatctg ctgacggctg cagccttcgg cacgatgaag
      181 aacagcgaaa atgagcttgc tgagcaactt atagaacaaa ccggcgataa cactctgacg
      241 ttaa
//
LOCUS       DAZFJL010000425          241 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZFJL010000425 DAZFJL010000000
VERSION     DAZFJL010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.428"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..>241
                     /locus_tag="ACHJ8C_004925"
     CDS             <1..>241
                     /locus_tag="ACHJ8C_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HFO5554594.1"
                     /translation="GSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPGRIQRKVFTRNEG
                     KQGPSLPYIPSGSFAKAMLIEGADANASVTGNESTV"
BASE COUNT           55 a           66 c           71 g           49 t
ORIGIN      
        1 agggcagcgt tgctgttcca ccgccgacgg cgttttatcc cgggaatggt gtcacgccac
       61 caccacaggt gacgtaccag tctgtgccgg tgcctggccg gatacagcgt aaggtgttca
      121 cccgtaatga gggaaaacag ggaccatcac tgccgtacat tccgtcagga agttttgcga
      181 aagccatgct gattgaagga gcggatgcca atgcctcagt caccggtaat gaatccacgg
      241 t
//
LOCUS       DAZFJL010000426          240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZFJL010000426 DAZFJL010000000
VERSION     DAZFJL010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.429"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
BASE COUNT          106 a           37 c           34 g           63 t
ORIGIN      
        1 agcctaattg gatgttcaca atgtccatta acagataaga taattaaaat gatagagcat
       61 gatctatcaa aaaacctaag tataatagat gttagcaaaa gattatttgt aagtgaatca
      121 tgcttacgca agcgattaaa aaagaacaac ttaactttca aaaggattag tctcgacaca
      181 aaaatgaaaa atgcctcaat acaactaaga acaaccagta atagcattta tatcattgca
//
LOCUS       DAZFJL010000427          240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZFJL010000427 DAZFJL010000000
VERSION     DAZFJL010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.denovo.430"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(<1..>240)
                     /locus_tag="ACHJ8C_004926"
     CDS             complement(<1..>240)
                     /locus_tag="ACHJ8C_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5554595.1"
                     /translation="SDEYMSIMTPLMKCERSVHDNNRYVTGKDPLLLDYPPSGNQIHF
                     HVFPDESATLVLYFSNNDIECFVFERDIPSQYRFF"
BASE COUNT           74 a           44 c           55 g           67 t
ORIGIN      
        1 tgaaaaagcg atactggctc ggaatatctc tttcaaagac gaaacattca atatcattgt
       61 ttgaaaaata aagaactagt gttgctgatt cgtcagggaa aacatggaaa tgtatctggt
      121 taccagaagg aggatagtcc agaagaaggg gatctttgcc cgtcacatac ctgttgttgt
      181 cgtggacgct acgctcacac ttcatcagag gtgtcatgat actcatgtat tcatctgatg
//
LOCUS       DAZFJL010000428        14278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.guided.119, whole genome shotgun sequence.
ACCESSION   DAZFJL010000428 DAZFJL010000000
VERSION     DAZFJL010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.guided.119"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            <1..261
                     /locus_tag="ACHJ8C_004927"
     CDS             <1..261
                     /locus_tag="ACHJ8C_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HFO5554596.1"
                     /translation="GADHYLSAELVMVVASDKEAEKIKHQEPLYQSIAVECLTEMKFE
                     DLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            281..997
                     /locus_tag="ACHJ8C_004928"
     CDS             281..997
                     /locus_tag="ACHJ8C_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HFO5554597.1"
                     /translation="MLQDVFESEEFAATLVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWQELAEEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLAGRELEDQFVTQQMLQHALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            1010..1873
                     /gene="motA"
                     /locus_tag="ACHJ8C_004929"
     CDS             1010..1873
                     /gene="motA"
                     /locus_tag="ACHJ8C_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO5554598.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            1876..2799
                     /gene="lafU"
                     /locus_tag="ACHJ8C_004930"
     CDS             1876..2799
                     /gene="lafU"
                     /locus_tag="ACHJ8C_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO5554599.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPSKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPENKVMQVSAMADQMLLDAKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRAR"
     gene            2870..3925
                     /gene="dinB"
                     /locus_tag="ACHJ8C_004931"
     CDS             2870..3925
                     /gene="dinB"
                     /locus_tag="ACHJ8C_004931"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO5554600.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVTLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKSDLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            3922..4374
                     /locus_tag="ACHJ8C_004932"
     CDS             3922..4374
                     /locus_tag="ACHJ8C_004932"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5554601.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITCIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            4565..5704
                     /locus_tag="ACHJ8C_004933"
     CDS             4565..5704
                     /locus_tag="ACHJ8C_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO5554602.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAALFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRNSLGSIGGGNHFAELQQIDQIIDAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARINRQL
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            5701..6315
                     /gene="prfH"
                     /locus_tag="ACHJ8C_004934"
     CDS             5701..6315
                     /gene="prfH"
                     /locus_tag="ACHJ8C_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO5554603.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(6372..7829)
                     /gene="pepD"
                     /locus_tag="ACHJ8C_004935"
     CDS             complement(6372..7829)
                     /gene="pepD"
                     /locus_tag="ACHJ8C_004935"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO5554604.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKAHGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            8090..8548
                     /gene="gpt"
                     /locus_tag="ACHJ8C_004936"
     CDS             8090..8548
                     /gene="gpt"
                     /locus_tag="ACHJ8C_004936"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO5554605.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            8640..9884
                     /gene="frsA"
                     /locus_tag="ACHJ8C_004937"
     CDS             8640..9884
                     /gene="frsA"
                     /locus_tag="ACHJ8C_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO5554606.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            9942..10343
                     /gene="crl"
                     /locus_tag="ACHJ8C_004938"
     CDS             9942..10343
                     /gene="crl"
                     /locus_tag="ACHJ8C_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO5554607.1"
                     /translation="MTLPSGHPKSRLVKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(10382..11437)
                     /gene="phoE"
                     /locus_tag="ACHJ8C_004939"
     CDS             complement(10382..11437)
                     /gene="phoE"
                     /locus_tag="ACHJ8C_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO5554608.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            11726..12829
                     /gene="proB"
                     /locus_tag="ACHJ8C_004940"
     CDS             11726..12829
                     /gene="proB"
                     /locus_tag="ACHJ8C_004940"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO5554609.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            12841..14094
                     /gene="proA"
                     /locus_tag="ACHJ8C_004941"
     CDS             12841..14094
                     /gene="proA"
                     /locus_tag="ACHJ8C_004941"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO5554610.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSESILN
                     ANAQDVADACANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
BASE COUNT         3522 a         3471 c         3847 g         3438 t
ORIGIN      
        1 ggcgcggatc attatttgtc ggcggaatta gtgatggtgg tcgccagtga caaagaggcg
       61 gaaaaaataa agcatcagga gccgttgtat caaagtattg cggttgaatg cctgacggaa
      121 atgaaatttg aagatttgcg gggcatgaaa atatctgcca tccgcaaact gatttctgac
      181 gcgttaaaga aagatcttca gcgtcgcaaa atgagcgcac cgtataaaga tttgctggtg
      241 aaaaaagtgg tcttccagta atcaccaggc ggatataaac atgctacagg atgtattcga
      301 gtcagaagaa tttgctgcca cgctggtatt aactccgcag caggagagcc actacttgca
      361 ggcctattta ccgttggtgc gtaaagtggt gcggcaactt gccccgcagt gtaattgcat
      421 tattgaccgt caggatatgg aacaaacggc gttaatgggg ctgttgaacg ccattcgtcg
      481 ctatgggctg ccggacgaag gttttgcggg ctatgccgtg caccgtattc gcggtgcgat
      541 cctggatgag ctgcgcgctc tcgactggcg accgcgccag ctgcgacaga aatatcatca
      601 gatgaaagac ctgattcgcg aaacgcgtaa aaaactgggg cacgaaccgg agtggcagga
      661 gctggctgag gaagggatct cgcatgaaga ttatctcgaa tatcagcaac tggaaggggc
      721 ggaaacgctc gccagcctgg acgaactgct gaacggcgaa gggccaggca ttccgcttgc
      781 tggccgggaa ctggaagatc agtttgtcac ccagcaaatg ttgcaacacg cgctttcaca
      841 attaagcgaa aaagagcaac tgatcctctc catgtattac cagcatgaga tgaatctcaa
      901 agagattgcg ctggtattag ggctaaccga ggcccgaatt tgtcaactga ataaacagat
      961 cgcgcagaaa attcgtgatt ttgtctatcc gaattaaagt gaagaaacca tgcaaaaaat
     1021 attaggttta ctggtggtgc tcggttgcgt atttggcggt tattttgaag cgggcgggca
     1081 attagtggct atctggcaac cggcggaaat gatcattatt ttgggtgctg gtttcggggc
     1141 gatgattatt gggaacccga aacatgtgct gaaagagatt gcccatcaaa ttaaaggcgt
     1201 gatcagtaaa aaacagctcg ggccggagtt tcagcgtcag ctattaatgt gcctgtatga
     1261 gttgctcgaa atggtgcaaa acggcggcct gcgtatgctg gatcaacata ttgagcagcc
     1321 ggaagagagc accattttcc agaaataccc gctggtgtta acccagaagc ggctggtgac
     1381 ttttattgcc gataactttc gcctgatggc gatgggcaag attgacgccc atgaactgga
     1441 aggcattctc gatcaggaac tggacaccgc tgaagagtcg ctgttaacgc cgtcacgctc
     1501 gctgcaacgt accgccgaag cgatgccggg atttggtatt tgtgcagcag tgctcggcat
     1561 catcatcacc atgcagtcga ttgatggatc tatcgccctg atcggcctga aagtggcggc
     1621 ggctctggtg gggacgtttt taggcgtgtt tatctgctac tgcctgatgg acccgcttgc
     1681 caacgccatg gaacaacagg cgcgggcgga acattctttg ctggaatgcg tgcgcaccgt
     1741 gctggtggcg caggcaggcg gcaaaccgac gctgctggcg gtggacgccg ggcgtaagct
     1801 gctgcatctg gcgtccaaac cgaccttcgc caacctcgac gcgtgggtga atgcgatgct
     1861 ggagcaagaa taagcatgag aaagacgggc aaccgccgcg aacgtggggc aaaaaccacc
     1921 atcgtccggc ggcagatcaa aaagaatcat gccgggcatc acggcggggc gtggaaggtg
     1981 gcgttcgccg actttacttt ggcaatgatg gcgctattta tgacgctgtg gattgtcaac
     2041 agcgtcagta aatccgagcg tgaaagcatt atcgccgcgc tgcacgggca gtcgattttc
     2101 aatggtggcg ggctgtcgcc gttaaataaa atcagcccgt ctcatccgtc aaaacctgcc
     2161 accgtggcgg tgccggaaga gacggagaaa aaagcgcgcg acgtcaacga gaaaacggcg
     2221 ctgttgaaga agaaatccgc caccgagctt ggcgagctgg caaccagtat caataccatc
     2281 gcccgtgatg cgcatatgga agcgaatctg gagatggaga ttgttcccca gggattacgc
     2341 gtgctgatta aagacgacca gaaccgcaat atgtttgaac gcggcagcgc gaagattatg
     2401 ccgttcttta aaactttgct ggtggagctt gcgccagtgt tcgactcgct cgataacaaa
     2461 attattatta ccgggcatac cgatgcgatg gcctacaaaa acaatatcta caacaactgg
     2521 aacctttcgg gcgatcgcgc gctttcggct cgtcgggtgc tggaagaggc cggaatgccg
     2581 gaaaataaag tgatgcaggt aagcgcaatg gcggaccaga tgctgctgga tgccaaaaat
     2641 ccgcaaagcg cgggcaaccg gcgcattgag attatggtgc tgaccaaaag tgcgtccgat
     2701 acgctgtatc aatactttgg tcagcatggg gataaagtgg tgcagccgct ggtgcaaaag
     2761 ctggataagc agcaggtgct ttcgcagcga gcgcgttaaa tgctgaatct ttacgcattt
     2821 ctcaaaccct gaaatcactg tatactttac cagtgttgag aggtgagcaa tgcgtaaaat
     2881 cattcatgtg gatatggact gctttttcgc agcggtggag atgcgcgaca atcccgccct
     2941 gcgcgatatc cctattgcta ttggcggcag ccgcgaacga cggggggtaa tcagcaccgc
     3001 caattatccc gcgcgtaaat ttggcgtacg tagcgctatg ccgacaggga tggcgctcaa
     3061 attatgcccg catctcacct tgcttccagg gcgctttgac gcctacaaag aagcctcaaa
     3121 tcatatccgc gaaattttct cgcgctatac ctcgcgcatt gaaccgttat cactggatga
     3181 agcctatctc gatgtcaccg atagcgtcca ttgccacggt tctgcgaccc tcatcgccca
     3241 ggaaatccgc cagacgatct tcaacgagct gcaactgacg gcgtctgcgg gcgttgcgcc
     3301 ggtcaagttt ctcgccaaaa tcgcctccga catgaataaa cccaacggtc agtttgtgat
     3361 tactccggct gaggttccgg catttctgca aaccttaccg ctggcaaaaa ttcccggcgt
     3421 cggcaaagtc tcagcggcta aactggaagc gatggggcta cgaacctgcg gtgatgtgca
     3481 aaagtgtgat ctggtgactc tgctcaaacg ttttggcaaa tttggccgca ttttgtggga
     3541 gcgtagtcag ggcattgacg agcgcgacgt taacagcgaa aggttgcgaa aatccgtcgg
     3601 tgtagaacgt acgatggcgg aagatattca tcactggtct gaatgtgaag cgatcatcga
     3661 gcggctttac cctgaactgg agcgtcgttt ggcaaaggta aaacctgatt tgctgattgc
     3721 ccgccagggg gtgaaattaa agttcgacga ttttcagcaa accacccagg aacacgtctg
     3781 gccgcggctg aataaatctg atctaatcgc caccgcgcgt aaaacctggg atgaacgccg
     3841 cggcgggcgc ggtgtgcgac tggtggggct gcatgtgacg ttgctggacc cgcaaatgga
     3901 aagacaactg gtgctgggat tatgaataac atacaaataa gaaactatca gcctggtgat
     3961 tttcagcaac tatgcgctat tttccttcga gctgttacga tgacggcgag tcagcactat
     4021 tctccacagc aaattgccgc ctgggcgcag attgacgaat ctcgctggaa ggagaaactc
     4081 gcgaaatcac aagtgcgggt tgcagtcatt aatgcacaac cggttggttt tattacctgc
     4141 attggacatt atatcgatat gttatttgtt gaaccggaat acacccgacg cggggttgcc
     4201 agcgctttgt taaaaccatt gattaagtct gaatccgaac ttacggtgga cgccagcata
     4261 accgcaaagc ccttttttga acgttatggt tttcagacag ttaagcagca gcgcgttgaa
     4321 tgccggggaa cgtggtttac taatttttat atgcgatata aaccgcaaca ttaagtccag
     4381 cttgcaatga aaataacgcc cgtctagtat gtgcgcgttt tcttattcca caaactgcaa
     4441 ggaggtaaat catgacaaat cctttatcaa tgactctttg cagacctttc caggattaat
     4501 tctttttttc ttgccctgga tttgtctgcc atttcctgat ttttattcat aaatggaatg
     4561 gtacatgggc aaatatattc gtcccttatc cgatgcggta tttaccatcg catctgatga
     4621 cctgtggatc gagagtttag cgatccaaca attacacacc acggcaaatt tacccaacat
     4681 gcagcgcgta gtagggatgc cagatttaca ccccggacgc ggctacccga ttggcgcagc
     4741 gttattctct gttggtcgtt tttacccggc actggtcggc aatgatatcg gctgcggcat
     4801 ggcgctatgg caaacagata ttctcgctcg caaatacaac gccgataagt ttgaaaagcg
     4861 attatcagat ctggatgacg ttgctgaaga aagctggctg gaggaaaacc tgccgtcagc
     4921 atttgcacag catccgtggc gcaactcgct tggctccatc ggtggcggta accacttcgc
     4981 agaactgcaa cagattgatc aaattatcga cgcggaactg tttgcactgg ctggtctgga
     5041 tgcgcagcat ctgcaactgc tggttcatag cggctcgagg ggattggggc agtctatttt
     5101 acagcggcat attgcctcgt tttcgcatca tggtttgcct gaaggcagtg acgacgcgct
     5161 acggtatatt gcggaacatg acgatgcgct ggcgtttgcg cgtattaatc gccagctgat
     5221 cgctttgcgc ataatgcaac aagttaaggc caccggaagt ccggttctgg atgtggcgca
     5281 taactttgtt agcgcgtgcc aaatcggcga tcaacagggc tggttgcatc gtaaaggcgc
     5341 cacgccggat gacaacggtc tggtgattat tcccggttca cgcggcgatt actcctggct
     5401 ggttaaacct gttgcgaacg aaaaaacgct ccactcgctg gcgcatgggg cggggcgtaa
     5461 atgggggcgc accgagtgta aagggcgtct ggcagcgaaa tacacggcga cgcaactctc
     5521 gcggactgaa cttggcagtc gggtaatttg tcgcgataaa caactcatct ttgaagaagc
     5581 gccacaagcc tataaatcgg ctgaaagcgt ggtgcaatgt ctggtgctgg ctgggttaat
     5641 tattcctgtc gcgcgactgc gcccggtgct gacgctcaaa aacagtggag ggaaaaaagg
     5701 atgatcttgc tacaactctc ctctgctcag gggccggaag aatgttgtct cgcagtgaaa
     5761 aaagcactgg acaggctgat taaagaagct acccgacagg acgtcgcggt aacggtgctg
     5821 gaaacagaaa cgggtcgcta ctctgacaca ctgcgttcgg cgctgatttc tctggatggc
     5881 gataacgcat gggctctaag cgaaagctgg tgcggcacta tccagtggat ttgtccgagt
     5941 ccgtatcggc ctcatcatgg gcgcaaaaac tggtttctgg gcattgggcg ttttaccgct
     6001 gatgagcagg aacaatcgga tgcaatccgt tatgagacgc tgcgttcgtc agggccgggc
     6061 ggtcaacatg tcaataaaac cgactcggcg gtacgcgcca cgcatctggc atccggtatt
     6121 agcgtgaagg ttcagtcaga gcgcagtcag catgctaaca aacggctggc gcgattactg
     6181 attgcctgga agctggaaca acagcaacag gaaaatagcg cggcgctgaa atcgcagcgg
     6241 cgaatgttcc atcaccagat tgaacgtggc aacccgcgac gaacgtttac agggatggcc
     6301 tttatcgaag ggtaatgaag gatgaaactg ccggaaggcg attaaacgcc atccggcagc
     6361 aaatcaaata attacttcgc cggaatttct ttcagcagtt cagtcagaag cgtccagtaa
     6421 tgaccaacgc tttcgatatg aacttgctca tccggggagt gtggaccggt gatggttggc
     6481 ccgatagaca ccatgtccat ttccggatac ggttttttga acagaccaca ttcgaggccc
     6541 gcgtggataa tctggatgtt cggcgttttg ttgaacagac gctgataggt ttcacgtacc
     6601 agatgcatta ccggagagtt agcgtccggc tgccagccag gatatgcgcc ttttgcttcg
     6661 gttttcgcac ctgccagttt acccagtgaa tccagcatgc tcactacgta gtctttaccg
     6721 ctgtcgatca gagaacggat caggcagtga atttctacgt tattgtcggt catggtcacc
     6781 acaccgacgt tcagggaggt ttcgaccaca ccttttgcca cgtcggagtt gcggatcaca
     6841 ccgttcgggg tggcgttcag cagacgaata aaggtatcgc gagatttcgc aatcagggca
     6901 gctttatcgt tcgcaacaga gtccagcagc aatgccagat ttttctcttt ctctgccagt
     6961 tcgtttttta ggatctcctg ataggtatcc accagagatt tcagggcgtc gactttatca
     7021 gctgcgacag caatggtcgc aaaggcttca cgcgggatgg cgttacgcag tgtgccgccg
     7081 ttgaaatcaa taagacgcag gtccagttct tctgcatgac ccgccaggaa gcgcaccaac
     7141 agtttgttgg cattacccag gccaacgtgg atttcaccgc cggagtgacc gcctttcaga
     7201 ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa ccgcttcacg atctaaatgc
     7261 aggttggagg tgaagtcgat acctcccgca caacccatgt agatttcacc ttcttcttcg
     7321 gagtcggtgt taatcagaat atcagcctgc aaccagttgc tctgtaagcc aaaagcaccg
     7381 tccataccgg cttcttcggt catggtcagc agcacttcca gcgggccgtg aaccacgttt
     7441 tcgtcagcca gaaccgccag tgcagaggcc ataccaatgc cgttatccgc acccagcgtg
     7501 gtgccgtgtg ctttaaccca ttcgccatca atataaggct ggataggatc tttggtgaag
     7561 tcatgcacgg tgtcgttatt tttttgcggc accatatcga ggtgggcctg caagacgacc
     7621 ggtttacgat tttccatacc tgcggtagca ggtttacgaa tcaggatatt acctacctga
     7681 tcgcgttcga catggaaacc tttctctttt gcccaaccaa caatgtattc agcgagttgc
     7741 tcttcatgat aggacgggtg aggaatagaa cagattttgg caaaaatatc ccacagcggc
     7801 tgtggagata attgagacag ttcagacacg ttaagtctcc ttgtcgatca cccgcaaaac
     7861 agtattgcag gtcacagggt tagcagaaaa tgttgtcaac acaagacagg cttgcgagat
     7921 atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga
     7981 ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt
     8041 cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa
     8101 atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat
     8161 gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgg taccgggtgc
     8221 gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga
     8281 tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt
     8341 catcgttatt gatgacctgg tagataccgg tggtactgcg gttgcgattc gtgaaatgta
     8401 tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga
     8461 ttatgttgtt gatatcccgc aaaatacttg gatcgaacag ccgtgggata tgggcgtcgt
     8521 attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt
     8581 tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca
     8641 tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct
     8701 cgacgctagt ccgccgcttt agtcacggcg cacaactgcc tgtgcagtcg gctcttgatg
     8761 gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca
     8821 ttgacccccg tgaaatcctc gacgtccagg cgcgtattgt gatgagcgat gccgaacgta
     8881 ccgacgatga tttatacgat acggtgattg gctaccgcgg cggcaactgg atttatgagt
     8941 gggctaccca ggcgatggtg tggcaacaaa aagcctgtgc ggaagaagat ccgcaactca
     9001 gtggtcgaca ctggctgcat gcggctacgt tgtacaacat tgctgcctat cctcatctga
     9061 aaggagatga cctggccgag caagcgcagg ctttgtcaaa ccgcgcctat gaagaggccg
     9121 ctcagcgtct gccgggcacg atgcggcaga tggagtttac cgtacccggc ggtgcgccca
     9181 tcaccggctt tttgcatatg ccgaaaggcg atggcccgtt cccgacagta ttaatgtgtg
     9241 gtggtctgga tgcgatgcag acggactatt acagcctgta tgaacgttat tttgcgccgc
     9301 gcggcattgc tatgctgact attgatatgc cgtcggtggg cttttcttca aaatggaagc
     9361 tcacccagga ctccagcctg ttgcatcagc acgttttaaa ggcgctgcct aacgtaccgt
     9421 gggtggatca cactcgcgtc gcggcctttg gtttccgttt cggcgctaac gttgccgtgc
     9481 gtctggcata ccttgaatcg ccacgtctga aagcggttgc ttgtcttggt ccggtagttc
     9541 ataccctgtt gagtgatttt aagtgccagc aacaggtgcc ggaaatgtat cttgacgttc
     9601 tggccagtcg tttagggatg catgatgctt ccgatgaagc gttgcgcgtg gagttgaatc
     9661 gctattcatt aaaagtgcaa ggattgctgg gacgtcgctg cccaacgcca atgttatcag
     9721 gctactggaa gaacgatccg ttcagcccgg aagaggactc acgcttaatc acgtcgtcat
     9781 ctgctgacgg taaattatta gagatcccat ttaacccggt gtatcggaat tttgacaaag
     9841 gccttcagga aatcacagac tggatcgaaa aacgtttgtg ttaaaaattt gctaaatttt
     9901 accaatttgg taaaacagtt gcatcacaac aggagattgc aatgacgtta ccgagtggac
     9961 acccgaagag cagattagtc aaaaaattta ccgcactagg cccgtatatt cgtgaaggta
    10021 agtgcgaaga taatcgattc tttttcgatt gtctggctgt atgcgtcaac gtgaaaccgg
    10081 caccggaagt gcgtgagttc tggggctggt ggatggaact ggaagcgcag gaatcccgtt
    10141 ttacctacag ttaccagttt ggtctgttcg ataaagcagg cgactggacg agtgttcaga
    10201 taaaagatgc cgaagtggtt gaacgattgg agcacaccct gcgtgaattt cacgagaagc
    10261 tgcgtgagct gctggcgacg ctgaatctga agctggaacc ggcagatgat tttcgtgatg
    10321 aaccggtgaa gttaacggcg tgaatgaaat gtgccggatg catcacatcc ggcaatatcc
    10381 attaaaactg ataggtcatg ccaaccgcga caatatcatc attattaata ttcaatttgt
    10441 tatcgctatc cagttggttg attttataat caacaaacgc tgacatattt ttgttgaaat
    10501 aatacgtagc accaacatcg acataattga ccagatcttc atcaccgatg ccctcaatat
    10561 ctttcccttt cgataagaca taacccagcg atggacgcag accaaagtca aactggtatt
    10621 gagcgaccgc ttcaaagttc tgcgtcttat tggcaaagcc gccagagatt ggcgtcattt
    10681 tacgtgtttc agaatagaaa gttgccagat aaatattatt ggcatcgtat ttcagacctg
    10741 ttgcccatgc ttctgcacgc ttgcctgtgc cacggctttg caggttctgc tcgttggtgc
    10801 gatcagagtt ggtataggct ccactgatgg cgaaatcgct gccaccaaag tcatatgtca
    10861 atgacgtgcc gaagccatcg ccgttttgct ttttaacgtc gcggttttcg tttttccctt
    10921 gatattgcag ggttaagttc aggccatcga taacgccgaa gaagtcggtg ttccgatacg
    10981 tcgccagacc gctggcgcgt ttggtcataa agttgtcggt ctgcgccgag gagtcgccac
    11041 caaattccgg gaacatatcg gtccaggctt ccacgtcata caacgcgccc aggttacgac
    11101 cataatcgaa agaacccaaa tctttatatt tcaacccggc aaaagcgaga cgcgtttttt
    11161 gctgtgcagt atcactctcc gctttattac cggcaaactc agcttcccaa cgaccataac
    11221 cagtcagttg atcgttaatt tgtgtttcgc ctttaaaacc aaaacggata taactctggt
    11281 cgccatcttt actgtcatta tcactcatat aatgcatggc tttaactttg ccatagacat
    11341 ccagtttatt accgtctttg ttatatattt ctgcggcctg tacggataca gatgccacaa
    11401 tgcccatcac cactaatgcc agagtgctct ttttcatttt cattcctgat tttaattaac
    11461 gcgcgaatat tcagcgggag agtcccgttg aaaacaggaa agtttttaac ctgagattgt
    11521 taaagatata ttacagatta ataatattct taaaatgtgg taatttatta aatctgtaat
    11581 aaaagcgtaa acaactgccg ctacgcttgc tgatcccgcg caacaaaacg ccatgctttg
    11641 ctcgcagatg gttggcaacc gacgacagtc ctgctaaaac gttcgtttga tatcattttt
    11701 tcctaaaatt gaatggcaga gaatcatgag tgacagccag acgctggtgg taaaactcgg
    11761 caccagtgtg ctaacaggcg gatcgcgccg cctgaaccgt gcccatatcg ttgaacttgt
    11821 tcgccagtgc gcgcagttac atgccgccgg gcatcggatt gttattgtga cgtcaggtgc
    11881 gattgccgcc ggacgtgagc acctggggta cccggaattg ccagcgacta tcgcttcaaa
    11941 acagctgctg gcggcggtag ggcagagtcg actgattcaa ctgtgggaac agctgttttc
    12001 aatttacggc attcatgtcg ggcagatgtt gctgacccgt gctgatatgg aagatcgtga
    12061 acgcttcctg aacgcccgcg ataccctgcg tgcgttgctc gataacaata tcgttccggt
    12121 aattaatgag aacgatgctg tcgctactgc agagattaag gtcggtgata acgataacct
    12181 ttctgcgctg gcggcgattc tggcgggtgc cgataaactg ttgctgctga ccgatcaaaa
    12241 aggtttgtat accgctgacc cgcgcagcaa tccgcaggca gaactgatta aagatgtgta
    12301 cggcattgat gacgcactgc gcgcgattgc cggtgatagc gtttcaggcc tcggaactgg
    12361 cggtatgagc accaaattgc aggccgctga cgtggcttgc cgtgcgggta tcgacaccat
    12421 tattgccgcg ggcagcaagc cgggcgttat tggtgatgtg atggaaggca tttccgtcgg
    12481 tacgctgttc catgcccagg cgactccgct tgaaaaccgt aaacgctgga ttttcggtgc
    12541 gccgcctgcg ggtgaaatca cggtagatga aggggcaact gccgccattc tggaacgcgg
    12601 cagctccctg ttgccgaaag gcattaaaag cgtgactggc aatttctcgc gtggtgaagt
    12661 catccgtatt tgcaacctcg aaggtcgcga tatcgcccac ggcgtcagtc gttacaacag
    12721 cgatgcatta cgccgtattg ccggacacca ctcgcaagaa attgatgcaa tactgggata
    12781 tgaatacggc ccggttgccg ttcaccgtga tgacatgatt acccgttaag gagctggccg
    12841 atgctggaac aaatgggcat tgccgcgaag caagcctcgt ataaattagc gcaactctcc
    12901 agccgcgaaa aaaatcgtgt gctggaaaaa atcgccgatg aactggaagc acaaagcgaa
    12961 agtatcctca acgctaacgc gcaggatgtt gcagacgcgt gtgccaatgg ccttagcgaa
    13021 gcgatgcttg accgtctggc actgacgccc gcacggctga aaggcattgc cgacgatgtg
    13081 cgccaggtgt gcaatctcgc cgatccggtg gggcaggtaa tcgatggcgg cgtactggac
    13141 agcggcctgc gtcttgagcg tcgtcgcgta ccgctggggg tgattggcgt gatttatgaa
    13201 gcgcgcccga acgtgacggt tgatgttgct tccctgtgcc tgaaaaccgg taacgcggtg
    13261 atcctgcgcg gcggcaaaga aacctgtcgc actaacgctg caacggtagc ggtgattcag
    13321 gacgccctga aatcctgcgg cttaccggcg ggtgccgtgc aggcaattga taatcctgac
    13381 cgtgcgctgg tcagtgaaat gctgcgtatg gataaataca tcgacatgct gatcccgcgc
    13441 ggtggggctg gtttgcataa actgtgccgc gagcagtcga caatcccggt gatcacaggc
    13501 ggtataggcg tatgccatat ttacgttgat gaaagtgcag agatcgctga agccctgaaa
    13561 gtaattgtca acgcgaaaac tcagcgtccg agcacatgta atacggtaga aacgttgctg
    13621 gtgaataaaa acatcgctga tagcttcctg cccgcattaa gcaaacaaat ggcggaaagc
    13681 ggcgtgacgt tacacgctga tgcagccgcg ctggcgcagt tgcaggcagg ccccgcgaag
    13741 gtggtggctg ttaaagccga agagtatgac gatgagtttc tgtcattaga tttgaacgtc
    13801 aaaatcgtta gcgatctgga tgacgccatt gcccatattc gtgaacacgg cacgcaacac
    13861 tccgatgcga tcctgacccg cgatatgcgc aacgcccagc gttttgttaa cgaagtggat
    13921 tcctccgctg tttacgttaa cgcctctacg cgttttaccg acggcggcca gtttggactg
    13981 ggggcggaag tggcggtaag cacacaaaaa ctccacgcgc gcggcccaat ggggctggaa
    14041 gcactgacca cttacaagtg gatcggcatt ggtgattaca ccattcgtgc gtaaataaaa
    14101 ccgggtgatg caaaagtagc catttgattc acaaggccat tgacgcatcg cccggttagt
    14161 tttaaccttg tccaccgtga ttcacgttcg tgaacatgtc ctttcagggc cgatatagct
    14221 cagttggtag agcagcgcat tcgtaatgcg aaggtcgtag gttcgactcc tattatcg
//
LOCUS       DAZFJL010000429        14104 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.guided.121, whole genome shotgun sequence.
ACCESSION   DAZFJL010000429 DAZFJL010000000
VERSION     DAZFJL010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.guided.121"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHJ8C_004942"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHJ8C_004942"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO5554611.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="ACHJ8C_004943"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="ACHJ8C_004943"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO5554612.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="asd"
                     /locus_tag="ACHJ8C_004944"
     CDS             2040..3008
                     /gene="asd"
                     /locus_tag="ACHJ8C_004944"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO5554613.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVDLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="ACHJ8C_004945"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="ACHJ8C_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO5554614.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLDRIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     NDALNQEILQISSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="ACHJ8C_004946"
     CDS             complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="ACHJ8C_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO5554615.1"
                     /translation="MPHSIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWHIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHFSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNTMGMPSIAMWMQCGLVTIFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="ACHJ8C_004947"
     CDS             complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="ACHJ8C_004947"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO5554616.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9527..11335
                     /gene="frdA"
                     /locus_tag="ACHJ8C_004948"
     CDS             9527..11335
                     /gene="frdA"
                     /locus_tag="ACHJ8C_004948"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO5554617.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11328..12062
                     /gene="frdB"
                     /locus_tag="ACHJ8C_004949"
     CDS             11328..12062
                     /gene="frdB"
                     /locus_tag="ACHJ8C_004949"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO5554618.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12073..12468
                     /gene="frdC"
                     /locus_tag="ACHJ8C_004950"
     CDS             12073..12468
                     /gene="frdC"
                     /locus_tag="ACHJ8C_004950"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO5554619.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIMIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12479..12838
                     /gene="frdD"
                     /locus_tag="ACHJ8C_004951"
     CDS             12479..12838
                     /gene="frdD"
                     /locus_tag="ACHJ8C_004951"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO5554620.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            12901..14034
                     /locus_tag="ACHJ8C_004952"
     CDS             12901..14034
                     /locus_tag="ACHJ8C_004952"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO5554621.1"
                     /translation="MLKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
BASE COUNT         3501 a         3725 c         3655 g         3223 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc ctaaaaaatt ttttttgagt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccaactcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaagccgtca agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctaacatc gagatagcgg tagtggaagt
      421 aggcttccag ctccggcatg tatttaaaca ggaagcgacg gtcctgaccg atgctgttac
      481 cacaaatcgg cgattttccc gcaggcaccc actgttttaa gaattcgagc gttgccagtt
      541 cagcttcacg atcgcccatc gtgctcgctt tcacacgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcggactga tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtctgtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgttgg tgaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccagc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcagtgtt gacgcgcccg gatttttacg acggtgtgaa acctattgct gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gagttcgtca acgagcagat ggatatctac cgcaatatcg
     1441 gttatcgcgt attgatggtt tccagtcata ctcaggatgg gctgaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgttggcaaa tccagcctgc
     1561 tgaacgcatt gctgggtctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggcttggtca gcacaccacg accgccgcac gactgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc cccaggtgtg cgtgagtttg gtctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgtagg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt actggttatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcaggca
     2401 actatctgat ggcggatctc ttccgtaacg gtacgtttgt gaccacttac ctctccccac
     2461 gtgactacca ccgcgtacac atgccgtgta acggtattct gcgtgaaatg atctacgtgc
     2521 cgggcgatct cttctccgtt aaccatctca cggctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatctgc ctgttcgata ccgaatttgg cccgatggcg cagattctgg
     2641 tcggtgcgac gattgtaggc agcattgaga cggtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggcat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aactaggctc taccgttatc aacctgtttg
     2821 caccaggtaa agtggacctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga tgctgaacca gccccgcttc
     2941 ctgctgaaga aatcgaggca gaacacgacg ccagcccatt ggttgacgat aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcacccagg
     3121 aactggagca ggcaaaagcg gcgaaacctg cgcagccgga agtcgtagag gcgctccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga tcgcatcaag caatatcaag
     3241 aagtcattga taattatccg aaactttccg ccactctgcg cgcgcagtta aacaacatgc
     3301 gtgacgagcc acgcagcgtg tcgccgggaa tgtctaacga tgcgctgaat caggaaattc
     3361 tccagatcag cagtcagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgat gcccgccgtc
     3481 agttgaatga aatcgagcgc cgcctgggga cgcttaccgg caataccccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactccg cacgtctgaa agcgctcgtt gatgaactgg
     3601 agctggcgca gctatctgcc aataaccgcc aggaattagc gcgcttgcgc tctgagctgg
     3661 cggaaaaaga gagccagcag ctggatgcat atttgcaagc actacgcaac cagttgaata
     3721 gtcaacgcca actggaagcg gagcgggcgc tggaaagcac cgaattgctg gcagaaaaca
     3781 gtgccgattt gcctaaagac atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcgg
     3841 cgctgaatca acaggcacag cggatggatc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacatt acaggtccgg caggcgttga atactctgcg tgaacagtcg caatggctgg
     3961 gatcatctaa cctgctcggc gaagcgttgc gggcgcaggt ggcacggcta ccggaaatgc
     4021 caaaaccaca acagcttgat accgaaatgg cgcagttacg tgtgcaacgg ttacgttatg
     4081 aggatctgct taataaacag ccgctgctac ggcaaatcca tcaggccgac ggtcagccgc
     4141 tgaccgctga gcaaaaccgt attctggaag cacagctacg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacgctac tgctggagct gaccaagctg aaagtctcca
     4261 acgggcaact ggaggatgcg ctgaaagagg tgaacgaagc gacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagtaagga gacgattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttacgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgctgcctgt gctgtggatg acactgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggtgat ggcgtaacag ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgctcgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgagtttc ccgtgggatg cgttactacc tgatgagcat cgggctcatt gtgccgctga
     4861 ttatggcgct gatgatgttc gataacctcg acgaccgtga attctccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt caccctcagc ctgaaaaagg
     4981 ccgggatccc gctgtatctc aacaaagagg gcagcggcga caacattacc aaccatatgc
     5041 tgtggaacat gatgattggc gcaccgctgg ttgccattct ggcgtcggcg gtgggttatc
     5101 tggcaacggc gcaggcgctg ttagcgaggc ttgaaacctc ggttgccatc tggtttttgc
     5161 tactggtggt ttatcacgtt atccgccgct ggatgctgat ccagcgccgc aggctggcgt
     5221 ttgaccgggc gaagcatcgc cgggcagaga tgttagcgca acgtgcgcgt ggcgaagagg
     5281 aagcgcatca tcacagtagc ccggaaggag caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat cagtgcgcaa tccttgcggc tggtgcgctc aattttgatg ttgatcgccc
     5401 tgctttcggt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttcgctgtg ggatgtcact tccacggtac agggcgtaga aagtctggag ccgattaccc
     5521 tcggtgcggt gctgattgcc attctggtgt ttatcatcac cacgcagctg gtgcgcaacc
     5581 tgcccgcact gctggaactg gcgattttgc agcacctgga tttaacgccg ggtacgggct
     5641 acgccatcac caccatcacc aaatatctgc tgatgctgat tggcgggctg gtcggcttct
     5701 caatgattgg tattgagtgg tcgaaattgc agtggctggt tgccgcgctc ggtgttggtc
     5761 tcggttttgg tttgcaggaa attttcgcca actttatctc tggcctgatc atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtaa cgatccgcga tcttaccggt agtgtgacga
     5881 aaattaacac ccgcgccacc accatcagcg actgggaccg caaagaaatt atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gtcacgcgtg
     6001 tggtgttgac aattccggcc cctgccgatg ccaacagtga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcactggtga tcgacaaccc agcaccggaa gtcttcctgg
     6121 tggatctgca gcaggggatt cagattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc gctacgccat gagatccacc aactaattct ggcgggcttc cacgcccacg
     6241 gtatcgatat gccattcccg cccttccaga tgcgtctgga aagcctcaac ggtaaacaaa
     6301 cggggagaac attaacttct gcgggcaaag gtcgtcaggc gggaagtttg taatgcatga
     6361 agctgccaaa tgatgtcaac gtggctcatt tagcagctta acatcatctt cagttatcac
     6421 tggccccgga gagtatcatc catacgcatt acttcctgac aactcatcgc cggagagacg
     6481 atgagttgca ggatttcata tgatcagtcg agagctaact caggtttgtt cgccatgcga
     6541 ctgcaatagt tctggtaaat cgccatcgct aacagcgaga agaagacagg gccgccaatc
     6601 atccacaatg tgctgtccca gtctccagct tcaaccacag gttgaatgat ggtgaagacg
     6661 ttcgcaaatg tcaccaccag tacgacaacc actgttgcaa tcattgccga catatgcgtt
     6721 ttgaaaatca caaacggtct gtcgagatcc tgacgtgctt taaaaaatgg gaaagccagc
     6781 gcgaggaaca ggtaaggaag cgtcatagac acgttcgcca tcagcgtcag cttgttaaag
     6841 aacgccgatg cggtaccgcc accaaacgaa accagcagaa tgaagatagt aaccaacccg
     6901 cactgcatcc acatggcaat agacggcatc cccatcgtat tcaggcgcgt catcggttcc
     6961 ggccacaatg ctttcggcgt tccctggatg atggctttca gcggtgaata gcaaagcgta
     7021 aagaacgcac cggtataagc gaggaacatc gaaagtccgg taatacgcgc aaaccatacg
     7081 cccagcgaca atgacgcttc aggtgaaaaa tgcagtgcgt tacccagcgt caccccgagg
     7141 ctcttcatca gcacataggt aatattgccg aggttaacgg aaccattact taatacctgc
     7201 tgccagtttg tgctgacgcc ccataaaaat attgccagcg aataaccgat tgaaataaca
     7261 atagcggcaa aaacaatacc tttggcaaag ttcttttctg gattttccgt tttatcaacc
     7321 agaccaccaa ccgcttcaat tccgccatag gcaaaaatgg caaataccac aaacgatagc
     7381 attgccagac cggactgata acctgggttc ggtgatgcaa ggaaattaat atcctgcgcg
     7441 aaatgcccac catttaataa caaaatagta atgcttacta acagcaatac taaattcaga
     7501 cacattactg caataccgcc caccgcagta atgcgggcaa ttttattaat cccttttgaa
     7561 gcaacgacgg tgaccagaat catccatgcc actgccagca gaccaaccac ctgcgtaggc
     7621 tccagtccgg caatatgcca gtgctgggtc atgtcgctac cgtagaggaa tgttgagaac
     7681 ggtacccaaa ctttcgcgga ggtactcacc atccagatga tataagagga aaaccacata
     7741 aacgtaccaa tgaaggcaaa acgtggtccg acactattat tcatccagga atagataccg
     7801 ccttcttctt tgcgataagc agctcccatt tcagccatca ttaaggcgaa tggaataaag
     7861 aataacaatg cagaaaatat ataaaaggga atcgcactat aacccattaa gtaataagcc
     7921 gatgggctat tggcaaatcc aaatacagaa gtaaagatca tcaatatgag tcctatcaga
     7981 ctcatctttt ttatcgagtg aggcatgaaa ccatccttca tttcagcgcg tgtataaaac
     8041 gcgcccactg aactgcactg cccaacattg agcgggcagc gtaaaaacca atacagaatt
     8101 gatacctgga tgatatcaaa tatccagtct taaattgtgg cctttagcgg aaaaatgaaa
     8161 acatatgcta cagaatggcg cttatcacgc cattcttcgc tgttaattca gtaatttttc
     8221 agaattatgc ccggtcaacg ctaaaggcga tgacttcagc cagtgtctcc gcgcccagcg
     8281 ccaacatcac cagacgatca acacctaatg ccacgccgga acagtcaggc ataccgactt
     8341 tcaaggcttc aatcagattc tggtcaatgg ggtgctgcgg caaaccgcgc gccgcgcgct
     8401 tacggttatc ttgttcaaag cgttgttgct gctcacgggc atccgtcaat tcatggaaac
     8461 cattcgccag ctcaatacct ttataataaa cctcaaagcg ttcagcgacc cgatgatctt
     8521 cggtactgat ttgcgccagt gatgcctggc tggctggaaa gtggtacaca aaggtcggtt
     8581 tttctttgcc aatatttggc tctacgccaa aggtaaacag caattgtaga agcgtgtcgc
     8641 ggtcttcttc agtatcggca acgttgctca aatccagttt cgctgccact tcccgcagtt
     8701 gcgttttgtc ggcagagagt gggtcaattt ccagataacg caagaaagct tgttgataag
     8761 aaaggctttc tgctgccgga cagtccagca cctgttgcaa gagatcgtcc acctcgttca
     8821 tcaaccggta catatcatag tgcggtcgat accactcaag catagtgaac tcagggttgt
     8881 gataacgccc catctcttca ttacggaagc tgcggcacag ctggaatacc ggcccacaac
     8941 cggcaaccag caggcgcttc atatggtatt ccgggctggt cattaaccag agattcatcc
     9001 cctgcgaatg cccggggcca acgaaacgtg tctcaaacgg gaccaaatga atatcggtta
     9061 ccgtcgcctg gctcatacaa ggcgtctcca cctccagcac tccacgatcg gcaaagaaac
     9121 gacggatctc cgccataatc gccgcgcgtt ttaataagtt aggaatggat gcgctcggct
     9181 gccaggatgc cgtttcgctc atagttaaat ctccagtttt tgacaagggc acgaagtcta
     9241 ctcgcaacgc gacggcgaga caaattttac gcaggaataa cacaacggtg ggcagtgact
     9301 aaaaaaagca cgatctgatg gtttagtaat taaattaatc atcttcagtg ataatttagc
     9361 cctcttgcgc actaaaaaaa tcgatctcgt caaatttcag acttatccat cagactatac
     9421 tgttgtacct ataaaggagc agtggaatag cgttcgcaga ccgtaacttt caggcactta
     9481 ccctgaagta cggggctgtg ggataaaaac aatctggagg aatgtcgtgc aaacctttca
     9541 agccgatctt gccattgtag gcgccggtgg cgcgggatta cgtgctgcaa ttgctgccgc
     9601 gcaggcaaat ccaaatgcaa aaatcgcact aatctcaaaa gtatacccga tgcgtagcca
     9661 taccgttgct gcagaagggg gctccgccgc tgtcgcgcag gatcatgaca gcttcgaata
     9721 tcactttcac gatacagtag cgggtggcga ctggttgtgt gagcaggatg tcgtggatta
     9781 tttcgtccac cactgcccaa ccgaaatgac ccaactggaa ctgtgggggt gcccatggag
     9841 ccgtcgcccg gatggtagcg tcaacgtacg tcgcttcggc ggcatgaaaa tcgagcgcac
     9901 ctggttcgcc gccgataaga ccggcttcca tatgctgcac acgctgttcc agacctctct
     9961 gcaattcccg cagatccagc gttttgacga acatttcgtg ctggatattc tggttgatga
    10021 tggtcatgtt cgcggcctgg tagcaatgaa catgatggaa ggcacgctgg tgcagatccg
    10081 tgctaacgcg gtcgttatgg ctaccggcgg tgcgggtcgc gtttatcgtt acaacaccaa
    10141 cggcggcatc gttaccggtg acggtatggg tatggcgcta agccacggcg ttccgctgcg
    10201 tgacatggaa ttcgttcagt atcacccaac cggcctacca ggttccggta tcctgatgac
    10261 cgaaggctgc cgcggtgaag gtggtattct ggtcaacaaa aatggctacc gttatctgca
    10321 agattacggc atgggcccgg aaactccgct gggcgagccg aaaaacaaat atatggaact
    10381 gggtccacgc gacaaagttt ctcaggcctt ctggcacgaa tggcgtaaag gcaacaccat
    10441 ctccacgcca cgtggcgatg tggtttacct cgacctgcgt cacctcggcg agaaaaaact
    10501 gcatgaacgt ctgccgttca tctgcgaact ggcgaaagcg tacgttggcg tcgatccggt
    10561 taaagaaccg attccggtac gtccgaccgc acactacacc atgggcggta tcgaaaccga
    10621 tcagaactgt gaaacccgca ttaaaggtct gttcgccgtg ggtgaatgtt cctctgttgg
    10681 tctgcacggt gcgaaccgtc tgggctccaa ctcgctggcg gaactggtgg tcttcggtcg
    10741 tctggccggt gaacaagcga cagagcgtgc agcaactgcc ggtaatggca acgaagcggc
    10801 aattgaagcg caggcagctg gcgttgaaca acgtctgaaa gatctggtta accaggatgg
    10861 cggcgaaaac tgggctaaga tccgcgacga aatgggcatg gccatggaag aaggctgcgg
    10921 tatctaccgt acgccggaac tgatgcagaa aaccatcgac aagctggcag agctgcagga
    10981 acgcttcaag cgcgtgcgca tcaccgacac ttccagcgtg ttcaacaccg acctgctcta
    11041 caccattgag ctgggccacg gtctgaacgt tgctgaatgt atggcgcact ccgcaatggc
    11101 acgtaaagag tcccgcggcg cgcaccagcg tctggacgaa ggttgcaccg agcgtgacga
    11161 cgtcaacttc ctcaaacaca ccctcgcctt ccgcgatgct gatggcacga ctcgcctgga
    11221 gtacagcgac gtgaagatta ctacgctgcc gccagctaaa cgtgtttacg gtggcgaagc
    11281 ggatgcagcc gataaggcgg aagcagccaa taagaaggag aaggcgaatg gctgagatga
    11341 aaaacctgaa aattgaggtg gtgcgctata acccggaagt cgataccgca ccgcatagcg
    11401 cattctatga agtgccttat gacgcaacta cctcattact ggatgcgctg ggctacatca
    11461 aagacaacct ggcaccggac ctgagctacc gctggtcctg ccgtatggcg atttgtggct
    11521 cctgcggcat gatggttaac aacgtgccaa aactggcatg taaaaccttc ctgcgtgatt
    11581 acaccgacgg tatgaaggtt gaagcgttag ctaacttccc gattgaacgc gatctggtgg
    11641 tcgatatgac tcacttcatc gaaagtctgg aagcgatcaa accgtacatc atcggcaact
    11701 cccgcaccgc ggatcagggt actaacatcc agaccccggc gcagatggcg aagtatcacc
    11761 agttctccgg ttgcatcaac tgtggtctgt gctacgccgc gtgcccgcag tttggcctga
    11821 acccagagtt catcggtccg gctgccatta cgctggcgca tcgttataac gaagatagcc
    11881 gcgaccacgg taagaaggag cgtatggcgc agttgaacag ccagaacggc gtatggagct
    11941 gtactttcgt gggctactgc tccgaagtct gcccgaaaca cgtcgatccg gctgcggcca
    12001 ttcagcaggg caaagtagaa agttcgaaag actttcttat cgcgaccctg aaaccacgct
    12061 aaggagtgca acatgacgac taaacgtaaa ccgtatgtac ggccaatgac gtccacctgg
    12121 tggaaaaaat tgccgtttta tcgcttttac atgctgcgcg aaggcacggc ggttccggct
    12181 gtgtggttca gcattgaact gattttcggg ctgtttgccc tgaaaaatgg cccggaagcc
    12241 tgggcgggat tcgtcgactt tttacaaaac ccggttatca tgatcattaa cctgatcact
    12301 ctggcggcag ccctgctgca caccaaaacc tggtttgagc tggcaccaaa agcggccaat
    12361 atcattgtaa aagacgaaaa aatgggacca gagccaatta tcaaaagtct ctgggcggta
    12421 actgtggttg ccaccatcgt aatcctgttt gttgccctgt actggtaagg agcctgagat
    12481 gattaatcca aatccaaagc gttctgacga accggtattc tggggcctct tcggggccgg
    12541 tggtatgtgg agcgccatca ttgcgccggt gatgatcctg ctggttggta ttctgctgcc
    12601 gctagggctg tttccgggtg atgcactgag ctacgagcgc gttctggcgt tcgcgcagag
    12661 cttcattggt cgcgtattcc tgttcctgat gatcgttctg ccgctgtggt gtggtttaca
    12721 ccgtatgcac cacgcgatgc acgatctgaa aatccacgta cctgcgggta aatgggtttt
    12781 ctacggtctg gctgctatcc tgacagttgt cacgctgatt ggtgtcgtta caatctaacg
    12841 catcgccaat gtaaatccgg cccgcctatg gcgggccgtt ttgtatggaa accagacctt
    12901 atgctcaaaa cgacgctctg caccttatta attaccgcct cttgctccac atttgccgcc
    12961 cctcaacaaa tcaacgatat tgtgcatcgc acaattaccc cgcttataga gcaacaaaag
    13021 atccccggta tggcggtggc ggtaatttat cagggtaaac cttattactt tacctggggc
    13081 tatgcggaca tcgccaaaaa gcagcccgtc acacagcaaa cgttgtttga gttaggttcg
    13141 gtcagcaaaa catttacggg cgtgcttggt ggcgacgcta ttgctcgagg ggaaatcaag
    13201 ttaagcgatc ccacaacaaa atactggcct gaacttaccg ctaaacagtg gaatgggatc
    13261 acactattac atctcgcaac ctacaccgct ggcggcctgc cattgcaggt gccggatgag
    13321 gtgaaatcct caagcgactt gctgcgcttc tatcaaaact ggcagcctgc atgggctcca
    13381 ggaacacaac gtctgtatgc caactccagt atcggtttgt tcggcgcact ggctgtgaag
    13441 ccgtctggtt tgagttttga gcaggcgatg aaaactcgtg tcttccagcc actcaaactc
    13501 aatcatacgt ggattaatgt accgtccgca gaagaaaaga actacgcctg gggatatcgc
    13561 gaaggtaagg cagtacatgt ttcgccaggg gcgttagatg ctgaagctta tggtgtgaag
    13621 tcgaccattg aagatatggc ccgctgggtg caaagcaatt taaaacccct tgatatcaat
    13681 gagaaaacac ttcaacaagg gatacaactg gcacaatctc gctactggca aaccggcgat
    13741 atgtatcagg gtctgggctg ggaaatgctg gactggccgg taaatcctga catcatcatt
    13801 aacggcagtg acaataaaat tgcactggca gcacgccccg taaaaccgat tacgccccca
    13861 actcctgcag tacgcgcatc atgggtacat aaaacggggg cgaccggcgg atttggtagc
    13921 tatgtcgcgt ttattccaga aaaagagctg ggtatcgtga tgctggctaa caaaaactac
    13981 cccaatccag cgagagtcgc cgccgcctgg cagattctca acgctctaca gtaaaatttc
    14041 atcgggtccg aattttcgga ccttttctcc gcttttcctt gctgtcatct acacttagaa
    14101 aaaa
//
LOCUS       DAZFJL010000430         1863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2201W38863
            SAMN43267807-rid23066033.guided.273, whole genome shotgun sequence.
ACCESSION   DAZFJL010000430 DAZFJL010000000
VERSION     DAZFJL010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267807
            Sequence Read Archive: SRR30307255
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350573.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:17:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,690
            CDSs (with protein)               :: 4,690
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 78 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 37 of 181
            Pseudo Genes (multiple problems)  :: 45 of 181
            Pseudo Genes (short protein)      :: 1 of 181
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267807-rid23066033.guided.273"
                     /serotype="O132:H34"
                     /isolate="2201W38863"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx2f"
     gene            75..1034
                     /gene="stxA2f"
                     /locus_tag="ACHJ8C_004953"
     CDS             75..1034
                     /gene="stxA2f"
                     /locus_tag="ACHJ8C_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001476133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2f subunit A"
                     /protein_id="HFO5554622.1"
                     /translation="MRYILLKLVLFFCVCLSSASYADEFTVDFSSQKSYVDSLNSIRS
                     AISTPLGNISQGGVSVSVINHVPGGNYISLNVRGLDPYSERFNHLRLIMERNNLYVAG
                     FINTETNTFYRFSDFSHISVPDVITVSMTTDSSYSSLQRIADLERTGMQIGRHSLVGS
                     YLDLMEFRGRSMTRASSRAMLRFVTVIAEALRFRQIQRGFRPALSEASPLYTMTAQDV
                     DLTLNWGRISNVLPEYRGEEGVRIGRISFNSLSAILGSVAVILNCHSTGSYSVRSVSQ
                     KQKTECQIVGDRAAIKVNNVLWEANTIAALLNRKPQDLTEPNQ"
     gene            1047..1310
                     /gene="stxB2f"
                     /locus_tag="ACHJ8C_004954"
     CDS             1047..1310
                     /gene="stxB2f"
                     /locus_tag="ACHJ8C_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2f subunit B"
                     /protein_id="HFO5554623.1"
                     /translation="MKKMIIAVLFGLFSANSMAADCAVGKIEFSKYNEDDTFTVKVSG
                     REYWTNRWNLQPLLQSAQLTGMTVTIISNTCSSGSGFAQVKFN"
BASE COUNT          503 a          346 c          437 g          577 t
ORIGIN      
        1 gctaaaaaca ttccggactt cttgcgaggt attattcctg gcgctgccgg ttcagactga
       61 tggagtgtta atatatgcga tatatattat taaagctggt gttgtttttt tgtgtttgct
      121 tgtcttcagc atcttatgca gatgagttta ctgtggattt ctcttcgcaa aagagctatg
      181 ttgattcatt gaatagtata aggtcggcaa tatccactcc acttggaaat atatctcagg
      241 gtggtgtttc tgtttcagta attaatcatg ttccaggcgg aaactatata tcattgaatg
      301 ttagaggcct tgatccatat agcgagagat ttaaccacct ccgtttaata atggaacgga
      361 ataacttata tgttgcaggc tttattaata ctgaaacgaa taccttttac agattctccg
      421 atttctcaca tatttcagtg cctgatgtga taactgtttc catgacgacg gacagcagtt
      481 attcatcatt acagcgaatc gcagatctgg aacgtacagg gatgcagatt gggcgtcatt
      541 cactggttgg ttcatatctg gatttaatgg agttcagagg acgttccatg acccgcgcat
      601 catccagagc tatgctgcgt tttgtcacag tgatagcaga agctctgcga ttcagacaaa
      661 tacagcgggg attccgaccg gcgctgtctg aggcatctcc gctttataca atgacggctc
      721 aggatgttga ccttaccctg aactggggaa gaataagtaa tgttcttcca gagtacagag
      781 gagaggaagg ggtaagaatc ggtaggatat cttttaatag tctttctgcg attctcggaa
      841 gtgttgcggt catccttaat tgccactcaa ccggaagtta ttcagttcgt tccgtgagcc
      901 aaaaacagaa aacagaatgc cagattgttg gagacagggc ggccattaaa gtaaataatg
      961 ttttgtggga agcgaataca atcgctgctt tattaaatcg caagcctcag gatcttactg
     1021 aaccaaacca ataacagggg gtgaatatga agaagatgat tattgcagtt ttattcggtc
     1081 tcttttctgc taattccatg gcggcggatt gtgctgtagg aaaaattgag ttttccaagt
     1141 ataatgagga tgataccttt actgtgaagg tgtcaggaag agaatactgg acgaacagat
     1201 ggaatttgca gccattgtta caaagtgctc agctgacagg gatgactgta acaatcatat
     1261 ctaatacctg cagttcaggc tcaggctttg cccaggtgaa gtttaactga gaatctacgg
     1321 tttatttatg cgcgtctttt gtttctggac gcagatatta ttagtgttgt ggatgctgat
     1381 taaatttggt cagtgttttc gttaaagtca tataaataca ggggcgttca cgcccctttt
     1441 tggtctgtag ttgggtgaag atcatcactt tactttgctc aaataactca gcacttgccg
     1501 gtttaccctt aaggcaatac ctacgttcca taaaagaagc atcaattagg atgctcgtat
     1561 acattgttat gtgctttaaa tgttaactga tggggatgct gaaataccgg acatgtactg
     1621 cttgcgtgtc cctcacgctt cgaaaatcgg gcggtatgtc agaatcgtgg tgagtgtgaa
     1681 tatcgctctg taattctgag tgttttttca acagatgaat aagcagacat tgaactctgt
     1741 ttatttatca gtatgatttc tttggttccg agggaagggt caattatgta tccgggcatc
     1801 tcattcacac ccgaggaacc agcgccgact tagctcagta ggtagagcaa ctgacttgta
     1861 atc
//
